LOCUS       DAZGXS010000001       138169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGXS010000001 DAZGXS010000000
VERSION     DAZGXS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 138169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 138169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(142..717)
                     /gene="gmhB"
                     /locus_tag="ACHKG5_000001"
     CDS             complement(142..717)
                     /gene="gmhB"
                     /locus_tag="ACHKG5_000001"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0866194.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            905..1936
                     /gene="metN"
                     /locus_tag="ACHKG5_000002"
     CDS             905..1936
                     /gene="metN"
                     /locus_tag="ACHKG5_000002"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0866195.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1929..2582
                     /gene="metI"
                     /locus_tag="ACHKG5_000003"
     CDS             1929..2582
                     /gene="metI"
                     /locus_tag="ACHKG5_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0866196.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2622..3437
                     /gene="metQ"
                     /locus_tag="ACHKG5_000004"
     CDS             2622..3437
                     /gene="metQ"
                     /locus_tag="ACHKG5_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0866197.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQGGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3555..3959
                     /gene="rcsF"
                     /locus_tag="ACHKG5_000005"
     CDS             3555..3959
                     /gene="rcsF"
                     /locus_tag="ACHKG5_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0866198.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            3956..4663
                     /gene="tsaA"
                     /locus_tag="ACHKG5_000006"
     CDS             3956..4663
                     /gene="tsaA"
                     /locus_tag="ACHKG5_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0866199.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4774..6492
                     /gene="proS"
                     /locus_tag="ACHKG5_000007"
     CDS             4774..6492
                     /gene="proS"
                     /locus_tag="ACHKG5_000007"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0866200.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6545..7369
                     /gene="yaeF"
                     /locus_tag="ACHKG5_000008"
     CDS             6545..7369
                     /gene="yaeF"
                     /locus_tag="ACHKG5_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP0866201.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRVIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7569..8279)
                     /gene="nlpE"
                     /locus_tag="ACHKG5_000009"
                     /gene_synonym="cutF"
     CDS             complement(7569..8279)
                     /gene="nlpE"
                     /locus_tag="ACHKG5_000009"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0866202.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8293..8703)
                     /gene="arfB"
                     /locus_tag="ACHKG5_000010"
     CDS             complement(8293..8703)
                     /gene="arfB"
                     /locus_tag="ACHKG5_000010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0866203.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            complement(8700..9245)
                     /gene="yaeQ"
                     /locus_tag="ACHKG5_000011"
     CDS             complement(8700..9245)
                     /gene="yaeQ"
                     /locus_tag="ACHKG5_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0866204.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9411..9611
                     /gene="yaeP"
                     /locus_tag="ACHKG5_000012"
     CDS             9411..9611
                     /gene="yaeP"
                     /locus_tag="ACHKG5_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0866205.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9598..9858
                     /gene="rof"
                     /locus_tag="ACHKG5_000013"
     CDS             9598..9858
                     /gene="rof"
                     /locus_tag="ACHKG5_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0866206.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9911..11206)
                     /gene="tilS"
                     /locus_tag="ACHKG5_000014"
     CDS             complement(9911..11206)
                     /gene="tilS"
                     /locus_tag="ACHKG5_000014"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0866207.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(11271..11660)
                     /gene="yaeR"
                     /locus_tag="ACHKG5_000015"
     CDS             complement(11271..11660)
                     /gene="yaeR"
                     /locus_tag="ACHKG5_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0866208.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11717..13858)
                     /gene="ldcC"
                     /locus_tag="ACHKG5_000016"
     CDS             complement(11717..13858)
                     /gene="ldcC"
                     /locus_tag="ACHKG5_000016"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0866209.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13957..14916)
                     /gene="accA"
                     /locus_tag="ACHKG5_000017"
     CDS             complement(13957..14916)
                     /gene="accA"
                     /locus_tag="ACHKG5_000017"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0866210.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14929..18411)
                     /gene="dnaE"
                     /locus_tag="ACHKG5_000018"
     CDS             complement(14929..18411)
                     /gene="dnaE"
                     /locus_tag="ACHKG5_000018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0866211.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18448..19044)
                     /gene="rnhB"
                     /locus_tag="ACHKG5_000019"
     CDS             complement(18448..19044)
                     /gene="rnhB"
                     /locus_tag="ACHKG5_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0866212.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19041..20189)
                     /gene="lpxB"
                     /locus_tag="ACHKG5_000020"
     CDS             complement(19041..20189)
                     /gene="lpxB"
                     /locus_tag="ACHKG5_000020"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0866213.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20189..20977)
                     /gene="lpxA"
                     /locus_tag="ACHKG5_000021"
     CDS             complement(20189..20977)
                     /gene="lpxA"
                     /locus_tag="ACHKG5_000021"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0866214.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20981..21436)
                     /gene="fabZ"
                     /locus_tag="ACHKG5_000022"
     CDS             complement(20981..21436)
                     /gene="fabZ"
                     /locus_tag="ACHKG5_000022"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0866215.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21541..22566)
                     /gene="lpxD"
                     /locus_tag="ACHKG5_000023"
     CDS             complement(21541..22566)
                     /gene="lpxD"
                     /locus_tag="ACHKG5_000023"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0866216.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22570..23055)
                     /gene="skp"
                     /locus_tag="ACHKG5_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22570..23055)
                     /gene="skp"
                     /locus_tag="ACHKG5_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0866217.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23177..25609)
                     /gene="bamA"
                     /locus_tag="ACHKG5_000025"
     CDS             complement(23177..25609)
                     /gene="bamA"
                     /locus_tag="ACHKG5_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0866218.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25639..26991)
                     /gene="rseP"
                     /locus_tag="ACHKG5_000026"
     CDS             complement(25639..26991)
                     /gene="rseP"
                     /locus_tag="ACHKG5_000026"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0866219.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(27003..27860)
                     /gene="cdsA"
                     /locus_tag="ACHKG5_000027"
     CDS             complement(27003..27860)
                     /gene="cdsA"
                     /locus_tag="ACHKG5_000027"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0866220.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27873..28631)
                     /gene="ispU"
                     /locus_tag="ACHKG5_000028"
                     /gene_synonym="uppS"
     CDS             complement(27873..28631)
                     /gene="ispU"
                     /locus_tag="ACHKG5_000028"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0866221.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28820..30016)
                     /gene="ispC"
                     /locus_tag="ACHKG5_000029"
     CDS             complement(28820..30016)
                     /gene="ispC"
                     /locus_tag="ACHKG5_000029"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0866222.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            complement(30108..30665)
                     /gene="frr"
                     /locus_tag="ACHKG5_000030"
     CDS             complement(30108..30665)
                     /gene="frr"
                     /locus_tag="ACHKG5_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0866223.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30815..31540)
                     /gene="pyrH"
                     /locus_tag="ACHKG5_000031"
     CDS             complement(30815..31540)
                     /gene="pyrH"
                     /locus_tag="ACHKG5_000031"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0866224.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31687..32538)
                     /gene="tsf"
                     /locus_tag="ACHKG5_000032"
     CDS             complement(31687..32538)
                     /gene="tsf"
                     /locus_tag="ACHKG5_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0866225.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32796..33521)
                     /gene="rpsB"
                     /locus_tag="ACHKG5_000033"
     CDS             complement(32796..33521)
                     /gene="rpsB"
                     /locus_tag="ACHKG5_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0866226.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33889..34683
                     /gene="map"
                     /locus_tag="ACHKG5_000034"
     CDS             33889..34683
                     /gene="map"
                     /locus_tag="ACHKG5_000034"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0866227.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34745..37417
                     /gene="glnD"
                     /locus_tag="ACHKG5_000035"
     CDS             34745..37417
                     /gene="glnD"
                     /locus_tag="ACHKG5_000035"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0866228.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37448..38272
                     /gene="dapD"
                     /locus_tag="ACHKG5_000036"
     CDS             37448..38272
                     /gene="dapD"
                     /locus_tag="ACHKG5_000036"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0866229.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38587..38973
                     /gene="yaeH"
                     /locus_tag="ACHKG5_000037"
     CDS             38587..38973
                     /gene="yaeH"
                     /locus_tag="ACHKG5_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0866230.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39062..40219)
                     /gene="cdaR"
                     /locus_tag="ACHKG5_000038"
     CDS             complement(39062..40219)
                     /gene="cdaR"
                     /locus_tag="ACHKG5_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0866231.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40374..41798)
                     /gene="degP"
                     /locus_tag="ACHKG5_000039"
     CDS             complement(40374..41798)
                     /gene="degP"
                     /locus_tag="ACHKG5_000039"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0866232.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41928..43445)
                     /gene="dgt"
                     /locus_tag="ACHKG5_000040"
     CDS             complement(41928..43445)
                     /gene="dgt"
                     /locus_tag="ACHKG5_000040"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0866233.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43529..44227
                     /gene="mtnN"
                     /locus_tag="ACHKG5_000041"
     CDS             43529..44227
                     /gene="mtnN"
                     /locus_tag="ACHKG5_000041"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0866234.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44220..45020
                     /gene="btuF"
                     /locus_tag="ACHKG5_000042"
     CDS             44220..45020
                     /gene="btuF"
                     /locus_tag="ACHKG5_000042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0866235.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45058..45681
                     /gene="yadS"
                     /locus_tag="ACHKG5_000043"
     CDS             45058..45681
                     /gene="yadS"
                     /locus_tag="ACHKG5_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0866236.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45728..46072)
                     /gene="erpA"
                     /locus_tag="ACHKG5_000044"
     CDS             complement(45728..46072)
                     /gene="erpA"
                     /locus_tag="ACHKG5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0866237.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46065..46130)
                     /gene="yadW"
                     /locus_tag="ACHKG5_000045"
     CDS             complement(46065..46130)
                     /gene="yadW"
                     /locus_tag="ACHKG5_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0866238.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46154..47575)
                     /gene="clcA"
                     /locus_tag="ACHKG5_000046"
     CDS             complement(46154..47575)
                     /gene="clcA"
                     /locus_tag="ACHKG5_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0866239.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47587..47634)
                     /gene="yadX"
                     /locus_tag="ACHKG5_000047"
     CDS             complement(47587..47634)
                     /gene="yadX"
                     /locus_tag="ACHKG5_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0866240.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47800..49080
                     /gene="hemL"
                     /locus_tag="ACHKG5_000048"
     CDS             47800..49080
                     /gene="hemL"
                     /locus_tag="ACHKG5_000048"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0866241.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49115..51097)
                     /gene="fhuB"
                     /locus_tag="ACHKG5_000049"
     CDS             complement(49115..51097)
                     /gene="fhuB"
                     /locus_tag="ACHKG5_000049"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0866242.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51094..51984)
                     /gene="fhuD"
                     /locus_tag="ACHKG5_000050"
     CDS             complement(51094..51984)
                     /gene="fhuD"
                     /locus_tag="ACHKG5_000050"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0866243.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51984..52781)
                     /gene="fhuC"
                     /locus_tag="ACHKG5_000051"
     CDS             complement(51984..52781)
                     /gene="fhuC"
                     /locus_tag="ACHKG5_000051"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0866244.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52832..55075)
                     /gene="fhuA"
                     /locus_tag="ACHKG5_000052"
     CDS             complement(52832..55075)
                     /gene="fhuA"
                     /locus_tag="ACHKG5_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0866245.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55295..57829)
                     /gene="mrcB"
                     /locus_tag="ACHKG5_000053"
     CDS             complement(55295..57829)
                     /gene="mrcB"
                     /locus_tag="ACHKG5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0866246.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58025..60454)
                     /gene="hrpB"
                     /locus_tag="ACHKG5_000054"
     CDS             complement(58025..60454)
                     /gene="hrpB"
                     /locus_tag="ACHKG5_000054"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0866247.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60528..61058
                     /gene="thpR"
                     /locus_tag="ACHKG5_000055"
     CDS             60528..61058
                     /gene="thpR"
                     /locus_tag="ACHKG5_000055"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0866248.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            61073..61777
                     /gene="sfsA"
                     /locus_tag="ACHKG5_000056"
     CDS             61073..61777
                     /gene="sfsA"
                     /locus_tag="ACHKG5_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0866249.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61955..62410
                     /gene="dksA"
                     /locus_tag="ACHKG5_000057"
     CDS             61955..62410
                     /gene="dksA"
                     /locus_tag="ACHKG5_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0866250.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62477..63373
                     /gene="gluQRS"
                     /locus_tag="ACHKG5_000058"
     CDS             62477..63373
                     /gene="gluQRS"
                     /locus_tag="ACHKG5_000058"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0866251.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63433..64830
                     /gene="pcnB"
                     /locus_tag="ACHKG5_000059"
     CDS             63433..64830
                     /gene="pcnB"
                     /locus_tag="ACHKG5_000059"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0866252.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64827..65309
                     /gene="folK"
                     /locus_tag="ACHKG5_000060"
     CDS             64827..65309
                     /gene="folK"
                     /locus_tag="ACHKG5_000060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0866253.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            65590..66186
                     /locus_tag="ACHKG5_000061"
     CDS             65590..66186
                     /locus_tag="ACHKG5_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0866254.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66273..66998
                     /locus_tag="ACHKG5_000062"
     CDS             66273..66998
                     /locus_tag="ACHKG5_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0866255.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNLTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67040..69640
                     /locus_tag="ACHKG5_000063"
     CDS             67040..69640
                     /locus_tag="ACHKG5_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP0866256.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELIVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            69654..70202
                     /locus_tag="ACHKG5_000064"
     CDS             69654..70202
                     /locus_tag="ACHKG5_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0866257.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70217..70822
                     /locus_tag="ACHKG5_000065"
     CDS             70217..70822
                     /locus_tag="ACHKG5_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0866258.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            70843..71433
                     /locus_tag="ACHKG5_000066"
     CDS             70843..71433
                     /locus_tag="ACHKG5_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0866259.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71445..72554
                     /locus_tag="ACHKG5_000067"
     CDS             71445..72554
                     /locus_tag="ACHKG5_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0866260.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSVSGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            72689..73483
                     /gene="panB"
                     /locus_tag="ACHKG5_000068"
     CDS             72689..73483
                     /gene="panB"
                     /locus_tag="ACHKG5_000068"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0866261.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73495..74346
                     /gene="panC"
                     /locus_tag="ACHKG5_000069"
     CDS             73495..74346
                     /gene="panC"
                     /locus_tag="ACHKG5_000069"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0866262.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74645..75541)
                     /gene="rpnC"
                     /locus_tag="ACHKG5_000070"
     CDS             complement(74645..75541)
                     /gene="rpnC"
                     /locus_tag="ACHKG5_000070"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP0866263.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            75815..76195
                     /gene="panD"
                     /locus_tag="ACHKG5_000071"
     CDS             75815..76195
                     /gene="panD"
                     /locus_tag="ACHKG5_000071"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0866264.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76199..77428)
                     /gene="yadE"
                     /locus_tag="ACHKG5_000072"
     CDS             complement(76199..77428)
                     /gene="yadE"
                     /locus_tag="ACHKG5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0866265.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77492..77932)
                     /gene="yadI"
                     /locus_tag="ACHKG5_000073"
     CDS             complement(77492..77932)
                     /gene="yadI"
                     /locus_tag="ACHKG5_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0866266.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(78037..78807)
                     /gene="yadH"
                     /locus_tag="ACHKG5_000074"
     CDS             complement(78037..78807)
                     /gene="yadH"
                     /locus_tag="ACHKG5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0866267.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78804..79730)
                     /gene="yadG"
                     /locus_tag="ACHKG5_000075"
     CDS             complement(78804..79730)
                     /gene="yadG"
                     /locus_tag="ACHKG5_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0866268.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79839..80501
                     /gene="can"
                     /locus_tag="ACHKG5_000076"
     CDS             79839..80501
                     /gene="can"
                     /locus_tag="ACHKG5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0866269.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80542..81078)
                     /gene="hpt"
                     /locus_tag="ACHKG5_000077"
     CDS             complement(80542..81078)
                     /gene="hpt"
                     /locus_tag="ACHKG5_000077"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0866270.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81284..83674
                     /gene="gcd"
                     /locus_tag="ACHKG5_000078"
     CDS             81284..83674
                     /gene="gcd"
                     /locus_tag="ACHKG5_000078"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0866271.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83876..85426)
                     /gene="cueO"
                     /locus_tag="ACHKG5_000079"
     CDS             complement(83876..85426)
                     /gene="cueO"
                     /locus_tag="ACHKG5_000079"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0866272.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85592..85939
                     /gene="yacC"
                     /locus_tag="ACHKG5_000080"
     CDS             85592..85939
                     /gene="yacC"
                     /locus_tag="ACHKG5_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0866273.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86045..86911
                     /gene="speE"
                     /locus_tag="ACHKG5_000081"
     CDS             86045..86911
                     /gene="speE"
                     /locus_tag="ACHKG5_000081"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0866274.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            86927..87721
                     /gene="speD"
                     /locus_tag="ACHKG5_000082"
     CDS             86927..87721
                     /gene="speD"
                     /locus_tag="ACHKG5_000082"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0866275.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87759..88121)
                     /gene="yacL"
                     /locus_tag="ACHKG5_000083"
     CDS             complement(87759..88121)
                     /gene="yacL"
                     /locus_tag="ACHKG5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0866276.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88297..90894)
                     /gene="acnB"
                     /locus_tag="ACHKG5_000084"
     CDS             complement(88297..90894)
                     /gene="acnB"
                     /locus_tag="ACHKG5_000084"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0866277.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91249..93102
                     /gene="yacH"
                     /locus_tag="ACHKG5_000085"
     CDS             91249..93102
                     /gene="yacH"
                     /locus_tag="ACHKG5_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0866278.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93173..94597)
                     /gene="lpdA"
                     /locus_tag="ACHKG5_000086"
     CDS             complement(93173..94597)
                     /gene="lpdA"
                     /locus_tag="ACHKG5_000086"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0866279.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94805..96697)
                     /gene="aceF"
                     /locus_tag="ACHKG5_000087"
     CDS             complement(94805..96697)
                     /gene="aceF"
                     /locus_tag="ACHKG5_000087"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0866280.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96712..99375)
                     /gene="aceE"
                     /locus_tag="ACHKG5_000088"
     CDS             complement(96712..99375)
                     /gene="aceE"
                     /locus_tag="ACHKG5_000088"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0866281.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99536..100300)
                     /gene="pdhR"
                     /locus_tag="ACHKG5_000089"
     CDS             complement(99536..100300)
                     /gene="pdhR"
                     /locus_tag="ACHKG5_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0866282.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            100841..102214
                     /gene="aroP"
                     /locus_tag="ACHKG5_000090"
     CDS             100841..102214
                     /gene="aroP"
                     /locus_tag="ACHKG5_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0866283.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(102257..103111)
                     /gene="ampE"
                     /locus_tag="ACHKG5_000091"
     CDS             complement(102257..103111)
                     /gene="ampE"
                     /locus_tag="ACHKG5_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0866284.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(103108..103659)
                     /gene="ampD"
                     /locus_tag="ACHKG5_000092"
     CDS             complement(103108..103659)
                     /gene="ampD"
                     /locus_tag="ACHKG5_000092"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0866285.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            103747..104640
                     /gene="nadC"
                     /locus_tag="ACHKG5_000093"
     CDS             103747..104640
                     /gene="nadC"
                     /locus_tag="ACHKG5_000093"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0866286.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAILCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            104843..105283
                     /gene="ppdD"
                     /locus_tag="ACHKG5_000094"
     CDS             104843..105283
                     /gene="ppdD"
                     /locus_tag="ACHKG5_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0866287.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            105293..106678
                     /gene="gspE"
                     /locus_tag="ACHKG5_000095"
     CDS             105293..106678
                     /gene="gspE"
                     /locus_tag="ACHKG5_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0866288.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            106668..107870
                     /gene="hofC"
                     /locus_tag="ACHKG5_000096"
     CDS             106668..107870
                     /gene="hofC"
                     /locus_tag="ACHKG5_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0866289.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(107905..108948)
                     /gene="guaC"
                     /locus_tag="ACHKG5_000097"
     CDS             complement(107905..108948)
                     /gene="guaC"
                     /locus_tag="ACHKG5_000097"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0866290.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(109104..109148)
                     /locus_tag="ACHKG5_000098"
     CDS             complement(109104..109148)
                     /locus_tag="ACHKG5_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866291.1"
                     /translation="MIIKTLKMSARKRN"
     gene            109173..109793
                     /gene="coaE"
                     /locus_tag="ACHKG5_000099"
     CDS             109173..109793
                     /gene="coaE"
                     /locus_tag="ACHKG5_000099"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0866292.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            109793..110536
                     /gene="zapD"
                     /locus_tag="ACHKG5_000100"
     CDS             109793..110536
                     /gene="zapD"
                     /locus_tag="ACHKG5_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0866293.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            110546..110743
                     /gene="yacG"
                     /locus_tag="ACHKG5_000101"
     CDS             110546..110743
                     /gene="yacG"
                     /locus_tag="ACHKG5_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0866294.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(110834..111232)
                     /gene="mutT"
                     /locus_tag="ACHKG5_000102"
     CDS             complement(110834..111232)
                     /gene="mutT"
                     /locus_tag="ACHKG5_000102"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0866295.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(111292..113997)
                     /gene="secA"
                     /locus_tag="ACHKG5_000103"
     CDS             complement(111292..113997)
                     /gene="secA"
                     /locus_tag="ACHKG5_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0866296.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(114059..114571)
                     /gene="secM"
                     /locus_tag="ACHKG5_000104"
     CDS             complement(114059..114571)
                     /gene="secM"
                     /locus_tag="ACHKG5_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0866297.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(114802..115719)
                     /gene="lpxC"
                     /locus_tag="ACHKG5_000105"
     CDS             complement(114802..115719)
                     /gene="lpxC"
                     /locus_tag="ACHKG5_000105"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0866298.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(115820..116971)
                     /gene="ftsZ"
                     /locus_tag="ACHKG5_000106"
     CDS             complement(115820..116971)
                     /gene="ftsZ"
                     /locus_tag="ACHKG5_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0866299.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(117032..118294)
                     /gene="ftsA"
                     /locus_tag="ACHKG5_000107"
     CDS             complement(117032..118294)
                     /gene="ftsA"
                     /locus_tag="ACHKG5_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0866300.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(118291..119121)
                     /gene="ftsQ"
                     /locus_tag="ACHKG5_000108"
     CDS             complement(118291..119121)
                     /gene="ftsQ"
                     /locus_tag="ACHKG5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0866301.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(119123..120043)
                     /gene="ddlB"
                     /locus_tag="ACHKG5_000109"
     CDS             complement(119123..120043)
                     /gene="ddlB"
                     /locus_tag="ACHKG5_000109"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0866302.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(120036..121511)
                     /gene="murC"
                     /locus_tag="ACHKG5_000110"
     CDS             complement(120036..121511)
                     /gene="murC"
                     /locus_tag="ACHKG5_000110"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0866303.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(121565..122632)
                     /gene="murG"
                     /locus_tag="ACHKG5_000111"
     CDS             complement(121565..122632)
                     /gene="murG"
                     /locus_tag="ACHKG5_000111"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0866304.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKATEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(122629..123873)
                     /gene="ftsW"
                     /locus_tag="ACHKG5_000112"
     CDS             complement(122629..123873)
                     /gene="ftsW"
                     /locus_tag="ACHKG5_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0866305.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(123873..125189)
                     /gene="murD"
                     /locus_tag="ACHKG5_000113"
     CDS             complement(123873..125189)
                     /gene="murD"
                     /locus_tag="ACHKG5_000113"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0866306.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(125192..126274)
                     /gene="mraY"
                     /locus_tag="ACHKG5_000114"
     CDS             complement(125192..126274)
                     /gene="mraY"
                     /locus_tag="ACHKG5_000114"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0866307.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(126268..127626)
                     /gene="murF"
                     /locus_tag="ACHKG5_000115"
     CDS             complement(126268..127626)
                     /gene="murF"
                     /locus_tag="ACHKG5_000115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0866308.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(127623..129110)
                     /gene="murE"
                     /locus_tag="ACHKG5_000116"
     CDS             complement(127623..129110)
                     /gene="murE"
                     /locus_tag="ACHKG5_000116"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0866309.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(129097..130863)
                     /gene="ftsI"
                     /locus_tag="ACHKG5_000117"
     CDS             complement(129097..130863)
                     /gene="ftsI"
                     /locus_tag="ACHKG5_000117"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0866310.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(130879..131244)
                     /gene="ftsL"
                     /locus_tag="ACHKG5_000118"
     CDS             complement(130879..131244)
                     /gene="ftsL"
                     /locus_tag="ACHKG5_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0866311.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(131241..132182)
                     /gene="rsmH"
                     /locus_tag="ACHKG5_000119"
     CDS             complement(131241..132182)
                     /gene="rsmH"
                     /locus_tag="ACHKG5_000119"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0866312.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(132184..132642)
                     /gene="mraZ"
                     /locus_tag="ACHKG5_000120"
     CDS             complement(132184..132642)
                     /gene="mraZ"
                     /locus_tag="ACHKG5_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0866313.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(133244..134248)
                     /gene="cra"
                     /locus_tag="ACHKG5_000121"
     CDS             complement(133244..134248)
                     /gene="cra"
                     /locus_tag="ACHKG5_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0866314.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(134330..134416)
                     /locus_tag="ACHKG5_000122"
     CDS             complement(134330..134416)
                     /locus_tag="ACHKG5_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0866315.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(134428..134919)
                     /gene="ilvN"
                     /locus_tag="ACHKG5_000123"
     CDS             complement(134428..134919)
                     /gene="ilvN"
                     /locus_tag="ACHKG5_000123"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0866316.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(134922..136646)
                     /gene="ilvI"
                     /locus_tag="ACHKG5_000124"
     CDS             complement(134922..136646)
                     /gene="ilvI"
                     /locus_tag="ACHKG5_000124"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0866317.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(136964..137908)
                     /gene="leuO"
                     /locus_tag="ACHKG5_000125"
     CDS             complement(136964..137908)
                     /gene="leuO"
                     /locus_tag="ACHKG5_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0866318.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        33490 a        37785 c        34002 g        32892 t
ORIGIN      
        1 caggattttt tctcttcaac cgaaccggct gtttgtgtga agtgattcac attcgccgtg
       61 tcgatggagg cgcattatag ggagtcggct caggaagaca agcggaaaaa tgcattttta
      121 tttcaaccgc tcatctttta atcattgcgc cggtttttgc tgctttttta tcgcttgcgg
      181 caggtctgcc aggctattta acacccaatc tgccgcgttt tctgcttcag gtgtaatagg
      241 tttacccgta cgcaccagca cttttgttcc cacgttcgcc gcaaccgctg cctgcatatc
      301 ttctaattta tcgcccacca tataagaagc ggccatatca atatgcaaat aatcgcgtgc
      361 tgacaaaagc atccccggat gtggtttgcg gcaatcgcag acctggcgaa actcttcaac
      421 actaccctgc ggatgatgcg ggcaataata gataccatcc agatcgacat ctcggtccgc
      481 cagcgaccag tccatccact cggtcagcgt ttcaaactgt gcttcggtaa atttaccgcg
      541 agcaatgcca gactggttgg tgactaccac cagcgcaaag cccatttttt ttagctcgcg
      601 catggcgtca ataacaccgt cgataaattc aaagttgtcg atctcatgga catagccgtg
      661 atcgacatta atggtgccat cacggtcaag aaaaattgcg ggtacgctct tcgccacctt
      721 ttatagctcc ttaataaggc atgtgacgct agtatcgcat gtttcgacct gcaagaaagt
      781 gctcttcgca taaacctgat tgatttagac gtctggatgc cttaacatcc atttcattga
      841 cggcgttgcc cgtttcaggc attcgagatg ccacgactaa cttaatgacg ataataaata
      901 atcaatgata aaactttcga atatcaccaa agtgttccac cagggcaccc gcaccatcca
      961 ggcgttgaac aacgtcagcc tgcatgtgcc agctggacaa atttatggcg ttatcggtgc
     1021 ctcaggcgcg ggtaagagta cgcttatacg ttgtgtaaac ctgctggagc gcccaaccga
     1081 gggtagcgtg ctggtcgatg gacaggaact gaccacgctg tcagaatccg agttgaccaa
     1141 agctcgccgc cagattggta tgattttcca gcattttaac ctgctctctt cgcgtactgt
     1201 ttttggcaac gtggctctgc cgctggagct ggacaataca ccgaaagacg agatcaaacg
     1261 tcgcgtgacg gaattgctgt cattggttgg tcttggagat aagcatgata gctacccgtc
     1321 gaatctttcc ggtgggcaga aacaacgtgt ggcgattgcc cgtgcattag ccagcaatcc
     1381 caaagtattg ctgtgtgatg aagccaccag cgcgctagac ccggcaacga cacgttctat
     1441 tctcgaactg ctgaaagaca ttaaccgccg tctgggtttg acgattctgt tgatcaccca
     1501 cgaaatggac gttgtgaagc gtatttgtga ttgcgttgcg gtcatcagca atggcgaact
     1561 gatcgagcag gacacggtaa gtgaagtgtt ctcacatccg aaaacgccgc tggcgcagaa
     1621 gtttattcag tcaaccctgc atctggatat cccggaagat taccaggaac gtctgcaagc
     1681 ggagccattt actgactgcg tgccgatgct gcgtctggag tttaccggtc aatcggtcga
     1741 tgccccactg ctttctgaaa ccgcgcgtcg tttcaacgtc aacaacaaca ttattagcgc
     1801 gcagatggat tacgccggtg gcgttaagtt cggcatcatg ctgactgaaa tgcacggcac
     1861 acaacaagat acgcaagccg ccattgcctg gctgcaggaa caccatgtaa aagtagaggt
     1921 actgggttat gtctgagccg atgatgtggc tgctggttcg tggcgtatgg gaaacgctgg
     1981 caatgacctt cgtatccgga ttttttgggt ttgtggttgg tctgccggtt ggcgtcctgc
     2041 tttatgtcac gcgtccgggg caaattattg ctaacgcgaa gctgtatcgt accgtttctg
     2101 cgattgtgaa cattttccgt tccatcccgt tcattatctt gctggtatgg atgattccgt
     2161 ttacccgcgt tattgtcggt acatcgattg gtttgcaggc agcaattgtt ccgttaaccg
     2221 ttggtgcagc accgtttatt gcccgtatgg tcgagaacgc tctgctggag atcccaaccg
     2281 ggttaattga agcttcccgc gcaatgggtg ccacgccaat gcagatcgtc cgtaaagtgc
     2341 tgttaccgga agcgttgccg ggtctggtga atgcggcaac tatcaccctg attaccctgg
     2401 tcggttattc cgcgatgggt ggtgcagtcg gtgccggtgg tttaggtcag attggctatc
     2461 agtatggcta catcggctat aacgcgacgg tgatgaatac ggtactggta ttgctggtca
     2521 ttctggttta tttaattcag ttcgcaggcg accgcatcgt ccgggctgtc actcgcaagt
     2581 aacgttcaac acaacataaa taattgaaga aggaataagg tatggcgttc aaattcaaaa
     2641 cctttgcggc agtgggagcc ctgattggat cactggcact ggtaggctgc ggtcaggatg
     2701 aaaaagatcc aaaccacatt aaagtcggcg tgattgttgg tgccgaacag caggttgcag
     2761 aagtcgcgca gaaagttgcg aaagacaaat atggcctgga cgttgagctg gtaaccttca
     2821 acgactatgt tctgccaaac gaagcattga gcaaaggcga tatcgacgcc aacgccttcc
     2881 agcataaacc gtaccttgat cagcaactga aagatcgtgg ctacaaactg gtcgcagtag
     2941 gcaacacttt tgtttatccg attgctggtt actccaagaa aatcaaatca ctggatgaac
     3001 tgcagggtgg ttcgcaggtt gccgtgccaa acgatccaac taaccttggt cgttcactgc
     3061 tgctgctgca aaaagtgggc ttgatcaaac tgaaagatgg cgttggcctg ctgccgaccg
     3121 ttcttgatgt tgttgagaac ccaaaaaatc tgaaaattgt tgaactggaa gcaccgcaac
     3181 tgccgcgttc tctggacgac gcgcaaatcg ctctggcagt tattaacacc acctatgcca
     3241 gccagattgg cctgactcca gcgaaaaacg gtatctttgt cgaagataaa gactccccgt
     3301 acgtaaacct gatcgtaacg cgtgaagata acaaagacgc cgagaacgtg aagaaattcg
     3361 tccaggctta tcagtctgac gaagtttacg aagcagcaaa caaagtgttt aacggcggcg
     3421 ctgttaaagg ctggtaattt ttaggctgtt tccacaattt gtaatatcat tcaggacggg
     3481 cgcttgcccg tcttgtcatt tttacaagct cctgattcaa tattgacgtt ttgatcatac
     3541 attgaggaaa tactatgcgt gctttaccga tctgtttagt agcactcatg ctaagcggct
     3601 gttccatgtt aagcagatcc cctgtcgaac ccgttcaaag cactgcaccc cagccgaaag
     3661 cggagcctgc aaaaccgaaa gcgccgcgcg ccacgccggt ccgaatttat accaatgcag
     3721 aagaattagt cggcaaaccg ttccgcgatc tcggtgaagt cagtggcgac tcttgccagg
     3781 cctctaatca ggactctccg ccgagcattc caaccgcgcg taagcggatg caaatcaacg
     3841 cctcgaaaat gaaagccaat gcggtattac tgcatagctg cgaagttacc agcggtacgc
     3901 caggctgcta tcgtcaggct ttatgtatcg gttctgcgct taacattacg gcgaaatgag
     3961 cagtttccag ttcgagcaaa taggtgttat tcgctcgccc tataaagaaa aattcgccgt
     4021 tccgcgccag ccaggtctgg taaaaagcgc caacggcgaa ctacatctca ttgctcccta
     4081 caaccaggct gacgccgttc gcggcctgga agcattcagc catttatgga tccttttcgt
     4141 ctttcatcaa acgatggaag gcggctggcg tccgactgtg cgtccgcctc gtcttggcgg
     4201 taatgccaga atgggtgttt tcgctacgcg ctccactttc cgccccaacc cgattggtat
     4261 gtcgctggta gagctgaaag aggttgtttg ccataaagac tgcgtgattc tgaagctcgg
     4321 cagcctggat ctggtcgatg gtacgccggt agtggatatc aaaccgtatc tcccctttgc
     4381 cgaatcgctt cccgatgcca gtgccagcta tgcgcaaagt gcgccagctg cagagatggc
     4441 ggtgagtttt accgcagagg tcgaaaagca gcttttgacg ctggagaagc gttatccgca
     4501 gttaacgctg tttatccgcg aagtactggc gcaggacccg cgcccggcct atcgtaaagg
     4561 tgaggaaacg ggcaaaacct atgccgtctg gctacatgat tttaacgttc gctggcgcgt
     4621 caccgacgca ggttttgaag tctttgcgct ggaaccgcgt taaattcacg cccttctctt
     4681 ttgacatttc ttttgcactg gtaaactaaa tcactttttt tgtcccaggc tcgccttgag
     4741 cctgttctac cttccaactg gaaccgtaac aacatgcgta ctagccaata cctgctctcc
     4801 actctcaagg agacacctgc cgacgccgag gtgatcagcc atcagctgat gctgcgcgcc
     4861 gggatgatcc gcaagctggc ctccgggtta tatacctggc tgccgaccgg cgtgcgcgtt
     4921 ctgaaaaaag tcgaaaacat cgtgcgtgaa gagatgaaca acgccggtgc aatcgaggtg
     4981 tcgatgccgg tggttcagcc agccgatttg tggcaagaga gtggtcgttg ggaacagtac
     5041 ggtccggaac tgctgcgttt tgttgaccgt ggcgagcgtc cgttcgtact cggcccaact
     5101 catgaagaag ttatcactga cctgattcgt aacgagctta gctcttacaa acagctgccg
     5161 ctgaacttct atcagatcca gaccaagttc cgcgacgaag tgcgtccgcg tttcggcgtc
     5221 atgcgttccc gcgaattcct gatgaaagat gcttactctt tccatacttc tcaggaatcc
     5281 ctgcaggaaa cctacgatgc aatgtatgcg gcctacagca aaatcttcag ccgcatgggg
     5341 ctggatttcc gcgccgtaca ggccgacacc ggttctatcg gcggcagcgc ctctcacgaa
     5401 ttccaggtgc tggcgcagag cggtgaagac gatgtggtct tctccgacac ctctgactat
     5461 gcagcgaaca ttgagctggc agaagctatc gcgccgaaag aaccgcgcgc tgctgctacc
     5521 caggaaatga cgctggttga tacgccgaac gcgaaaacca tcgcggaact ggttgaacag
     5581 ttcaatctgc cgattaagaa aacggttaag actctgctgg ttaaagcggt tgaaggcagt
     5641 agcttcccgc tggttgcgct gctggtgcgc ggtgatcacg agctgaacga agttaaagca
     5701 gaaaaactgc cgcaggttgc cagcccgctg actttcgcga ccgaagaaga aattcgtgcc
     5761 gtggttaaag ccggtccggg ttcactgggt ccggtaaaca tgccgattcc ggtggtgatt
     5821 gaccgtaccg ttgcggcgat gagtgatttc actgctggtg ctaacatcga tggtaaacac
     5881 tacttcggca tcaactggga tcgcgatgtc gctaccccgg aagttgcgga tatccgtaac
     5941 gtggtggctg gcgatccaag cccggatggc cagggtacgc tgctgatcaa acgtggtatc
     6001 gaagttggtc acatcttcca gctgggtacc aagtactccg aagcactgaa agcctccgta
     6061 cagggtgaag atggccgtaa ccaaatcctg actatgggtt gctacggtat cggggtaacg
     6121 cgcgtagtgg ctgcggcgat tgagcagaac tacgacgagc gcggcatcgt atggcctgac
     6181 gctatcgcgc cgttccaggt ggcaattctg ccgatgaaca tgcacaaatc cttccgcgta
     6241 caagagcttg ctgagaaact gtacagcgaa ctgcgtgcac aaggtatcga agtgctgctg
     6301 gatgaccgaa aagaacgtcc gggcgtgatg tttgccgata tggaactgat cggtattccg
     6361 cacactattg tgctgggcga ccgtaacctc gacaacgacg atatcgaata taaatatcgt
     6421 cgcaacggcg agaaacagtt aattaagact ggtgacatcg tcgaatatct ggtgaaacag
     6481 attaaaggct gatgccagaa agggtcctga atttcagggc ccttttttac atggattgta
     6541 taaaatggat aaaccaaagg cgtactgccg cctgcttctc ccctgctttc ttttactttc
     6601 ggcctgtacc gttgatatca gccagccaga ctcatcagcc acagcagtag atgccgaggc
     6661 gaaaacatgg gctgtcaaat tccagcatca aagctctttc accgaacaat caataaaaga
     6721 aataacggta cctgatctca aacccggcga tttgctgttc tcctcaagcc tggaggtaac
     6781 ctcatttggg atccgcgttt tcagcacttc ctctgtgagt cacgttgcaa tctatctggg
     6841 tgaaaataac gttgcagaag cgacaggcgc tggcgtccag attgtttccc ttaaaaaagc
     6901 gatgaagcat agtgataaac ttttcgtctt acgagtcccg gatcttaccc cgcaacaagc
     6961 cacagaaatc accgcgttcg ccaataaaat caaagatagc ggttataact atcgcgtcat
     7021 tgtcgaattt attcccttta tggtgactcg ccagatgtgc tcactgaatc cgttctccga
     7081 ggattttcgc caacagtgcg tcagcggcct ggcgaaagcg cagttaagca gtgtggtcga
     7141 aggagacaaa aagtcgtggt tttgttcgga atttgtcact gatgcatttg ccaaagccgg
     7201 gcatccgctt acgctggcgc aatcgggctg gattagccct gccgatctga tgcatatgcg
     7261 tattggtgat gtctctgcgt ttaagccaga aacgcagtta cagtatgttg ggcacctgaa
     7321 gccagggatt tatatcaagg cgggtcgctt tgttggtttg acccggtgat aaagtaaatg
     7381 ccttatccgc cttaaggtat gaggggggtt gtaggcatga taagacgcgc cagcgtcgca
     7441 tcaggcgttg agcaccattg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaaaac
     7501 gtgcaaattc aaaatgttga aatatatcct tctggcctgt tttgcgtttg tttctgtctc
     7561 aagacgggtt actgccccaa actactgcaa tcctggtttg ggtaaaattt ccctgccgta
     7621 tcgggtgcta atactttggt tggcgcaccg gtatccggat tagcctcaag cgtaaagtga
     7681 ccttctactg acagtaacac cggtttttca ctgtggccgc gcgcagccag gtagccacgc
     7741 tccagctctg cgttattcgc taccatgaaa cgttttccgg tcgcgcaatc agtgaaggtc
     7801 gccgcatcag ccatataaaa atacatgccc cgcagggtca tcggcgtcat gggtaaactg
     7861 gattgtgccg gttccagcgt atagttgaac tgcgattcaa tcggattgcc ttcacgatcg
     7921 agcatctcca gcgcatcgcc tttcgcccga taatatgact tttcaccttt gctgtcggtt
     7981 aataccagct tgtcagcggt tcgcgcccat gtaccgtagg aagcgaagga ggaaggttct
     8041 tcacgagcac cgagataacg ctcattcatc acccatgttc cgtctttttc gaggaacaga
     8101 gaggtttcga ttccttcgca atcggcacac ggcagcacgc cgcgccaact ttgcggcatc
     8161 ggtttcaatt cggcagcctg cgccggagaa agcgtatcga cttcggcccg attattacaa
     8221 cccatcagag taaagaggct gattacagcc atcgctgtca ctatcgcttt tttcaccatc
     8281 cattccttct ttttattccc gaccgctgcg catcgccttc acgcttgatt tttgtgcttt
     8341 cgatgccagc ctgcgctctt tcgatgcacg ggtgggccgc gtgggtcgtc gggctttttg
     8401 ttctgttgtt aaatctttaa tcactgccac cagccgggcc agcgccgctt cgcggttcag
     8461 ttcctgactg cggtattcct gtgccttaat gacaattacg ccatcactgc tgatcaaatg
     8521 atggctggcg gcgagcagac gctctttgta atactctggc aggctggatg cccgaatgtc
     8581 aaaacgcaga tgaatggccg ttgaggtctt attaacatgc tgcccgcccg cgccctgcgc
     8641 acgaatggcg gtgatctcaa gctcaccatc gggaatagca acatgtcggg aaatcacaat
     8701 catgaaggtt gttgccaggc ggttaagttc acttccagat tattcttatc atccgataac
     8761 cagatcacgc catcctgaat cgttgcctgc agcgtcatgg tacgatcggc aaaggcgctc
     8821 actttcgcca gctgctcgtc gtccagatac cagacggaaa gattggcaaa ctgcgcacat
     8881 ttgctctgat tttgctgcca ccagatttgc gccgcccgac tattataggt aaacagcgcc
     8941 acttctgcgg cctgggtgca ggctttctta attcgtcgct catccggcag ccccagctca
     9001 atccacaaat caatgcccag gtgatcgtta cgcagccacg cttccggctc atcatcggca
     9061 cacaaaccac gggtaaattg cagacgttca tcagcatatt tcagccacgc cagcaggcgc
     9121 agcatcatac gctcctgggt ttctgaaggg tggcgcgcca gcgtcagaga ggcatcgaga
     9181 aactggttgc ggtcgagatc ggccacatta accgtcgctt tataaattgt cgctttaagc
     9241 gccataagaa aactcctttc gaatcaggag gccattgtaa cggaatacgt gcagttgcgg
     9301 cgctgatatt gcagaaatga gtgtggtata gtcaccttgc gaaacagagt ttatcttcgt
     9361 aggcttagac ttgcatccac ggttaagtca gagtgctgac aggagggcat gtggaaaaat
     9421 attgtgagtt aatacgcaag cggtacgcgg aaatcgccag cggagactta ggatacgttc
     9481 cggacgcgct gggctgcgtt ttgaaagtgc tgaatgaaat ggctgccgac gacgcccttt
     9541 ctgaggctgt cagggaaaaa gcagcatacg ctgcggcaaa cttactggtg agcgattatg
     9601 tcaatgaatg atacgtatca accaatcaat tgtgatgatt acgataatct tgagctcgcc
     9661 tgccagcatc atttaatgct gacacttgag ctgaaagatg gcgaaaaatt gcaggccaaa
     9721 gccagcgatt tagtctcccg caaaaatgtg gagtatctgg tcgtcgaggc cgctggcgaa
     9781 acccgtgagc tacgtctgga taaaattacc agctttagcc acccggaaat cggtacggtg
     9841 gtggtgagcg agtcctgatt gactttcgcc ggatgcgacg cttaatgcgt catatccggc
     9901 cttcactaaa ctaagcgttt cgctgccaga caaaacttat gccattctca ccttcagcca
     9961 caccttcttg cgtcacaaat ccccctgccg ccgcaatcag cgtttcgcca taaaacagca
    10021 gtggcgtggt gtcacgtagc cacggcggca cgcccagctc ttgccagatt ttctttagct
    10081 tacgtccgcc gttacgcccg acaatatgca gcaatcctgg cgctttgaaa cgcacgctga
    10141 ccgcttcgtc tgcacgcgga gggcgaatat cgcctcccgc attaagctgt acacttccct
    10201 gccccgccgg taattccagc ggttgaagcc atgtctgcca cggcacaatg ttttcgcttt
    10261 gcccggtgac ggatttaatc caccacagtt gcgactgata gcgtcggatt tcaaacgcgc
    10321 ccaaacgtaa acagggggag gcatcttccc gcgccagcgc cacttcctgc cagatcctca
    10381 ccaacgcgtc gcgggaaggc atcggtgcat tctgccctgc cagccagcgg cggataatcg
    10441 ccgcgcggcg ggcatcactc atcgccagca ttggcacaat ctgcagcgtc ccctgcggcg
    10501 attgacagtg tgctaaatca tctgccagca gttcatccag caggctctct tgttcagcac
    10561 aaagtgcggc gctgcgggcc gttgtttcgg caaaatgcgg ccaacgctgc tgcaataacg
    10621 gcactacgcg caggcgcaga aagttacgat cgtatgagtc gtcctgatta ctttcgtctt
    10681 caatccagcg taaaccatgc gccaatgccc actgcgccag ttccccccgc gtgcgggcaa
    10741 gcaacgggcg gataagccgt gttccggcaa actccgaaac ttccgccata gccgaaagcc
    10801 cggcagggcc gctaccgcgt tttagcgcca gcagaaaagt ttcacattga tcgtcgagat
    10861 gttgcgcggt gaccagcact tcaccgggca acaaggtgcg ggcaaatgcc tgataacgcg
    10921 cctgccgcgc ctgggcctca atccccagcc cctcttgcgc aagttgtacg cgttcgacca
    10981 ccagcggcac ctgccactgt tggcagacgt tttcgcaatg cgtaacccag gcatcggcat
    11041 tggcacttaa accgtgatgc acatgaatag cgcgcagagc gccccccgga ttttccgtcc
    11101 gccactgcac caactgatgc agcagaacgg tggagtcaag cccgccgcta aaggccacca
    11161 gaatctggcg tgaggtgaga agttgtctat tgagcgtgag tgtcatgatg gtgcgatttt
    11221 acagtagcaa tgcccggcac gttaccgggc aaatgcggcg atgacaagcc ttactgctca
    11281 tacagttcca acggcagccc gtccggatcg ttaaagaagg tgaagcgttt ttgcgtgtat
    11341 ggatcgacac ggatggcttc acacttcacg ttatggcttt caaggtgcgc cactgccgca
    11401 tcgatgtcat caacgctaaa agccagatga cgcaggccgc aagcttccgg tcggctgggg
    11461 cgctccggcg ggaacggaaa tgagaaaagc tcaatcacat attgcccatt aagcgccaaa
    11521 tcccctttcc acgagtcgcg cgcttcgcga tagacttcgc tttgcagcgt gaaaccaaga
    11581 atatcgcagt agaaagcttt gctcaccgca taatccgtcg caataatcgc aatatggtga
    11641 acctgtttta aacccagcat aacgtctcct ttatttgtta acagcacgtt actcgcccgg
    11701 aagccgctct ggcaagttat cccgccattt ttaggactcg tacgcggtaa acgccgtctt
    11761 cgtcctgttt cgcaccgtga atatccgttt caaaaccggg gtaatgttgc cctacggaac
    11821 aaagcatcag tagaaaatcg agtactgtgc ggctctcttt ggtcagcatt tctccaggca
    11881 tcaacagcgg tacgcccggt ggataaggca ggatcatatt tgccgatact ctaccgacca
    11941 gttgttccag cgcaatggtt tctacttcac ctttaatctg tctctgccat gcctgatgtg
    12001 gcgtcatgat catctccggc aaagtatcga atgcccgcaa catcaaaccg ggaagatcgt
    12061 gtttacgaat cagcttatgg atcccttgcg ccagatcctg aatacgcata ttgcggtaga
    12121 aatcgggatc ttctgcgtac agatccggaa gcatattttt gattcgcaga ttgagatcgt
    12181 aagagcgttt aaattccgtc aacccacgca ataatcccat tgctttggtt ttatcgatgc
    12241 caatactaaa gagaaacagc aggttatatg ggccggtttt ctctaccacg atcccacgct
    12301 catcgaggaa cttcgccacc agcgccgccg ggatcccctc ctcgctcata ttgccctgct
    12361 cgtccatccc cggcgtcaga atagtgactt taaccggatc gaggaacata tgatcggcat
    12421 ccgcatcgtt aaagccgtgc cattgttcgc caggcgcaac gggccagcat tcggcttcat
    12481 ccacctgcgg cggttgccag atatcgaaaa accaaccgtc agactcttcc cgcagccgct
    12541 ggacctcttt gcgaaaatgc agagctcgtt ctactgaacg gttaatcagc cgtttgcccg
    12601 gattaccacg cagcatcgct gctgccgtct caaccgaagc aacaatggga taactgggcg
    12661 aggtggtggt atgcatcata aaggcttcgt taaacgcctc ttcgtcatac tcacctttaa
    12721 tgtggatcag cgaagcctgc gataacgccg ccagcatttt gtgggtcgat tgcgtttcga
    12781 agatcacttt tcccgcaaca cgctcgccgc tcataccact tttcccctgg tagatcggat
    12841 gaaaatgggt gtacggcacc caggcggaat cgaagtggat cgacgggaca tccagcgtct
    12901 gtttgatcca gtcggtgttg tagagcaagc catcataggt tgagttggta atcaccgcat
    12961 gaaccggcca ttgtgcctgc gtggtggcag cgactttctc ttcgatgctg tcgcgagtaa
    13021 attcacggcg cgggatccca cccagaatcc ccaacgcatt acgcgtcggt ttcagccaga
    13081 ctggcactac atcgttcatc atcaacagat gcgccagcga tttatgacaa ttgcggtcaa
    13141 tcaacagcgt actaccggat ggcgcggcgt acatacccac aattttgttc gacgtcgatg
    13201 ttccgttggt aacgatataa ctctgttccg cgccgaaagt ccgcgcgatg tactcttccg
    13261 cttccaggtg tggcccggtg tggtcgagca acgaaccaag ctcggtgacc gaaatagaga
    13321 catcagcctt aagggtattc ccgccgaaaa agtcataaaa cagacagcca accgggcttt
    13381 tttgatatgc ggtgccgccc atatgacccg gcgtacaaaa ggtgtacttc cgctctttga
    13441 cgtaggtaaa caaggctttc gtgaacggtg gggtaatgtt atcaagatat tcgtcggtgt
    13501 actgacgcat acgaatggcg atatcttccg cctgccccag cgcatattca aaaaaacaga
    13561 gcgccatccg catatcctgc acgctgacat ccatcgtcga gtgggtgttg atgaaggcat
    13621 aaagcgggag atattcatta agctgattga tatcgctaca taaatcgaga ctgtattcat
    13681 cccagtcaaa aatcacgccg caaattcgag ggttatgttc gataaatttc agcaaatcaa
    13741 cgctgttttg tggccagata atctgaaagc cttgcgccac cagcgccgac tccagttctt
    13801 tgatgggctc atctttataa aagacgccat gcggtcccat aatggcaatg atgttcatgt
    13861 gttcctcctg gaaaatcctt ccttaatcat agcctgctca aaccgtggcg ataaaaaagg
    13921 gccaccgaag tgaccctttt tcagaacttt tgcgaattac gcgtaaccgt agctcatcag
    13981 gcgctgataa cgacgatttt ttaaatcttc agtgcttaac acgtcgagat cggccagatc
    14041 cgccagcagt tgcgctttca acgatgccgc catcgcttcc gggttacggt gagcgccgcc
    14101 cagcggttcc gggatgatgg agtcgatcag tttcagctct ttcagacgcg gagcgatgat
    14161 acccatcgct tcagccgcca gcggcgcttt gtcggcgctc ttccacagaa tggacgcaca
    14221 accttccggc gagataacgg aataggtgct gtattgcagc atattcactt tatcgcccac
    14281 gccaatcgcc agcgcaccgc cagaaccacc ttcaccgata accgtacaaa ctaccggtac
    14341 gccgaggcga gacatttcac gcaggttgcg tgcaatggct tcagactgac cacgttcttc
    14401 tgcgcccacg cccggataag cccccggggt gtcgataaag gtgatgatag gcatcttaaa
    14461 gcgttcagcc atttgcatca gacgcagtgc tttgcggtaa ccttctggcg ctggcatacc
    14521 aaagttacgg cgaatttttt ctttggtttc acgacctttt tgatgaccaa tgatcatcac
    14581 cggacgacca tcgagacggg cgataccacc gacgatagct ttatcgtctg catacgcgcg
    14641 gtcgccagcc agttcgtcaa attcatcaaa tgccaggcga acgtaatcca gggtataagg
    14701 acgctgtgga tggcgtgcca gttgcgcaat ctgccatgca ccgagatcgg cgaagatttt
    14761 acgcgtcagt tctacgcttt tttcacgcag acgatgcact tcttcatcga tgttaatatc
    14821 cagtttctca tcctgacggc taaccgcagt cagagaatcg attttcgctt ccagctctgc
    14881 aatcggctgt tcaaaatcaa ggaaattcag actcatagta ttcctgtatt agtcaaactc
    14941 cagttccacc tgctccgaac caatgaggcc acggagatcg tttaataaac gatcgctcgg
    15001 agagacacgc cacgtcgcgc caaaacgcaa ccgcgcgcgt gcatccgccc tctgatagta
    15061 gagatgtact ggaattgtcc cagagcggtg gggttccaga gactgacgga gtcggtttaa
    15121 aagctggtca tcaatttgcc tgtccgtcag cgagatagca agcccgcgag catatttttc
    15181 ccgggcttcg tcaatatcca tcacttcgcg agcggtcatt ttaagcccac cgctgaagtc
    15241 atcaaagctg acctgtccgc tgacgataag tatgcggtct ttttccagca aatgctggta
    15301 tttatccagg gcgtcagtaa acaacatcac ttccagccgc ccggaacggt catccagcgt
    15361 gcagataccg atacgattgc cgcgcttggt gaccataacc cgcgcggcaa caacgagccc
    15421 cgcagccgtg atgactttac cacgttctgt cgggtgcatg tctttcagcc ttacgcctcc
    15481 gacataacgc tcaatctctt ttaaatactg gttgataggg tgtccggtca ggtacaggcc
    15541 taacgtttca cgttccccat ctaataccac ctgctccggc cacggttggc agctggcata
    15601 ggattgttca atttgttccg gctcttcggc cagcacgccg aacatatcgg cctgaccgat
    15661 agcttccgct ttcgcgtgtt gatctgccgc ttttaacgca tcgcccagcg agttcatcag
    15721 cgccgcgcga tgtggcccaa gacggtcaaa cgccccggac atgatcagtt tttccagcac
    15781 gcgacggttc aactttttgg tgtcggttcg ggcgcagaga tcaaacagtt cgcggaagta
    15841 gccgccttta ttacgggctt cgatgatcgc ctcaatcgga ccttcaccta cccctttgat
    15901 cgcgccaata ccatacacga tttcgccgtc gtcgttgacg tggaaatggt aaagaccgga
    15961 gtttatatct ggtggcagga ttttcagccc catccgccag cactcatcca ccaggcccac
    16021 caccttctcg gtgttgtcca tatcggcggt cattaccgcc gccataaact ctgccggata
    16081 gtgcgctttc aaccataatg tttgatatga caccaaagca taggccgcag agtgcgattt
    16141 gttaaatccg taaccagcga atttctccac caggtcgaag attttcatcg ccagttcggc
    16201 gttgattccg ttcttttctg caccttcagc aaatacagaa cgttgcttag ccatctcttc
    16261 cggctttttc ttacccatcg cacgacgcag catatccgcg ccaccgaggg tataaccaga
    16321 aagcacctgc gcaatctgca tgacctgttc ctgatacagg ataatgccgt aggttggctc
    16381 cagtaccggt ttcaggcttt catgctgcca ctgcacgtcc ggataggaga tctcttcgcg
    16441 accatgttta cggtcgataa agttatccac catccctgat tgcaacggac cagggcggaa
    16501 cagtgccacc agtgcgatca tatcttcgaa gcagtcaggt tgcagacgct tgatcaggtc
    16561 cttcatgccg cgcgattcaa gctggaatac cgccgtggtt tccgagcgtt gcagcatgtc
    16621 gaagcttttc ttgtcatcca gcgggatcgc ggcgatatcc agcggcggct cgccattctt
    16681 cgcccgccgc ttgttgatca tctccagcgc ccagttgatg atggtgagcg tacgcaaacc
    16741 aaggaagtcg aacttcacca gtccggcgta ttcaacgtcg cttttatcaa actgggtgac
    16801 cggatgtttg ccctcttcat cgcaataaag cggcgcaaaa tcggtaattt tggtcggcgc
    16861 gataaccacc ccaccggcgt gcttaccggc gttacgggtg accccttcca gtttgcgcgc
    16921 catgtcgatc aacgccttaa cttcttcatc cgcttcgtag atttccggca gctgcggctc
    16981 ggcttcaaac gctttcgcca gcgtcatccc cggatcgggc gggatcagtt tcgagatacg
    17041 atcgacaaag ccgtacggat gccccagcac gcggcctacg tcgcggatca ccgctttcgc
    17101 cgccatcgta ccgaaggtga taatctgcga taccgcatca cgaccgtaca tgtccgctac
    17161 gtgttcgata acctgatcgc gtttctccat acagaagtca acgtcgaagt caggcatgga
    17221 gacacgttcc gggttaagga aacgttcgaa cagcaggtca aattccagcg gatcgaggtc
    17281 ggtgattttc agcgcgtagg ccaccagtga acccgcaccg gagccacggc ctggccctac
    17341 cggtacgccg ttatctttcg accactggat aaattccata acgatgagga agtagcccgg
    17401 gaagcccatc tggttgataa cctgaagttc agtctccaga cgttcgtcat attccgggcg
    17461 gcgcttaaca cgttcttcct catcagggaa taaaaaggcc agacgctctt ccaggccctc
    17521 ttttgcacgc ttgaccagat aatcttcggt gctcatgtcc ccggtcggga actgcggcag
    17581 gaagtattca ccaagacgca cagttacgtt acagcgtttg gcgatctcaa cggtgttggc
    17641 aagggcttct gggatgtcgg caaacagctc gcacatctcc tcttcgctac gcatatattg
    17701 ctgcggcgaa tagttacgcg ggcgtttagg atcgtcgagg gtaaagccgt cgtggatcgc
    17761 gacgcggatt tcgtgtgcgt caaagtcgct gctgtcgata aagcgcacat cgttagtcgc
    17821 cacgacgggc aaaccgcgcg cttccgccag ttccaccgcc gcgtgcagat agctttcttc
    17881 atccggcctg ccggtgcgga tcagctcgag aaaatagcga tccgggaagt gttcttcata
    17941 aaacgcgaca cactcatcta ccagcgcgct gttaccacgc aaaagactgc gtccgacgtc
    18001 gcccatgcgc ccgccggaaa gaaggatcaa cccttcgttt aattcgataa gccagtcgcg
    18061 atcgatgatc ggcccggcgg caccgtaccc gcgctgatac gcttttgaga tcagcaacgt
    18121 cagattctga tagccggtat tgttcgccgc cagtaccgtc aggtgggtta actcatcacc
    18181 cagcaggtcg cactggacgt taaaatctgc cccgacgata ggcttaatcc ctgcgccatg
    18241 tcccgctccg tagaacttca ccagaccgca aaggttggtg aaatcggtga tcgccagtgc
    18301 tggcataccc aacgccgccg ccttttttac cagcggtgcg gttttggcca ggccatcgat
    18361 catcgagtag tcgctgtgca cccgcaggtg tacgaaacgt ggttcagaca tcttcagatt
    18421 ccggtttact taatctcgac acaagaatca ggacgcaagt cccagtgcgc gtttgacagg
    18481 cccaaagctg cgccgatggt gttcggtcgc gccgtgttca gccagttttt ccagatgaaa
    18541 agcggttgga taccctttgt gttgggcaaa accatattgc gggaaaacaa tatccagcgc
    18601 cgccatttcg gcgtcacgcg tcactttcgc caggatagac gcggcactga tttccggtac
    18661 gcggctatcg cctttcacca cagccatcgc aggcatcggt aatttcgggc agcggttacc
    18721 atcaatcaac acatattccg gcgcaatatg cagcccagcg acggctcgct gcatcgccag
    18781 catggtcgca tgtaaaatat tcagttcgtc gatttcgtga ggttccgcgc ggcccagact
    18841 ccagctcaac gctttctctt tgatctcttc acagagcgcc agacgacgtt tttcgctcag
    18901 ctttttggaa tcattcagcc cggcaatcgg gcgcgccggg tcgagaatca ccgcagcggt
    18961 gacgaccgcg ccaactaacg gcccgcgtcc gacttcatcc acacccgcaa ccagctgcgt
    19021 gtgcggatag acaaattcga tcattgtgct aactccagaa cggcttgtgc cgcctgctca
    19081 tcggcattgc agcggatctg ctgatgcagt tcacggaagg tatcgtgcat cgcgtggctg
    19141 gttttcccat tcgccaacag cggtaacagc gccgcagcca gtttttgcgg ctcacactct
    19201 tcctgcaata actctttgac taactctctg cccgccagca gatttggcag cgagacataa
    19261 tcagttttca ccagccgctt cgccaaccag aaggtaaaag gcttcatgcg atatcccacc
    19321 accatcgggc atttcgccag catacactcc agggctgccg tccccgacgc cagtagtgcc
    19381 gcatcgctgg cgaccatcgc ctcacggccc attccatcca gcaaatgaac cgacaggtct
    19441 ggcgcgactg cagctttgat gcgttcaaac tgctcgcggc gtttggcatt caccagtggc
    19501 accacgattt cgagatccgg atatgtctgg cgcaaaagct gggccgtttt caggaaatcg
    19561 gcactaagca tttcgacttc tgcaccacgg ctgcccggca acaatgccag acagtgggca
    19621 tcgtaaggga tccccagcac atcacgggca ctatttttat ctggatctaa tggcatggca
    19681 tcagccatgg tatgaccgat aaagcggcac ggtacgttgt atttgtcata aaacgctttt
    19741 tcgaaaggca gaaatgcgag caccagatcg gtggctctgc ctattttgaa aacgcgtttc
    19801 tgtcgccacg cccagacgga cggactgaca taatgaatgg ttttgatacc ctgcttttta
    19861 aggttacctt cgaggataat attgaagtca ggcgcatcaa tgccaacaaa aacatctggc
    19921 tttagttcac caaaacgctt tgtcagatcg gcacgaatat gcagtaagcg acgcagacga
    19981 ccgagcactt caacaatgcc catcaccgcc aactcttcca tttcgtacca ggcttcgcag
    20041 ccttcagcct gcattcgtgg cccggcaaca ccaacaaagc gggcgttggg cacacgttct
    20101 ttcagagcgc ggattaaacc ggcccccagg atatcgccgg aggtttctcc ggcgaccagg
    20161 gcaatcgtta atggacgctg ttcagtcatt aacgaatcag accgcgcgtt gagcgtgcaa
    20221 agaaatcggt aaaggctttc acttccggat atgtttccgc cagttcagca atttccggtt
    20281 tcacttcatc gagcgtttta ccgctacgat aaatcagctt atacgcattg cggatagcgg
    20341 taatcgcctc acggctgaat ccgcggcgct tcagcccttc gatattgaca ccgaacggcg
    20401 tggcgtggtt accctgcgca atgacataag gagggacgtc ctgcgccaca ccggagcagc
    20461 cgccaaccat cacatgcgca ccaatgatgc agaactgatg gactgcggcc atgccgccga
    20521 tgatcgcgaa gtcgtcaacc gatacgtgac ccgccagcgt tgcgttgttg gcgagaatac
    20581 agcggttacc taccgtacaa tcgtgcgcaa tgtgcgcgtt gatcatcagt aagttgtcgc
    20641 tgcccacctt cgtcaatcca ccgccctgga ctgtgccacg atgaatggtg acgctttcgc
    20701 gaatgcggtt acgatcgccg atttccacgc gggtcggttc gccagcatat ttcagatcct
    20761 ggttaacttc gccgatggag gcgaactgat aaatctcatt atcgcggcca attttagtat
    20821 gaccattcac gacaacgtga gatttcagta cggtaccctc accaatttcg acatggggtc
    20881 caacgataca aaaaggacca atgtgtgcgt tcgcgccaat cgacgcgccc tcttccacaa
    20941 tggcggttgg atgcacaaag gcggatttat caatcacgta tcaggcctcc cggctacgag
    21001 cacacatcat cgttgcttcg caaactactt taccgtcgac cagagcaacc cctttaaaac
    21061 gggtcaggcc gcggcgcgtt ttttcgaaag tgacttccat gatcatttga tcgccaggca
    21121 cgaccgggcg cttgaagcgc gcttcgtcaa taccagcgaa gtagtacagc tcacccggtt
    21181 ccagttttcc tacgctttta aacgccagaa tacctgttgc ctgtgccatt gcttccagaa
    21241 tcagcacacc cgggaaaatc ggttttccag ggaaatggcc ctggaagaat ggctcattga
    21301 cagagacatt ttttactgcg cgcagaaaac gaccttcttc aaaatccagc acgcgatcca
    21361 ccagtaagaa cgggaaacgg tgcggcagaa gttctaaaat ctcttcaatc tgcagagtat
    21421 gagtgttagt agtcaagata ctcttcctgt caaaatataa gaaacgacaa taataacacg
    21481 gcctgccgca atcgtaagaa tgagacaggc cgtaaagttt ggcgaacaaa agatggaacg
    21541 ttagtcttgt tgattaacct tgcgctcaag cgatttcaga cgcttgctca tgtcatcaat
    21601 gttcatcacc agtgcagcgg ttttgcgcca gactttgttg ggttgcagcg gaatgcctga
    21661 ggaatagacg cctggttcag tgatgggacg catcaccata cccatgcccg taaccgtcac
    21721 tttgtcgcat atttccatat gcccgttgat tacgctggct ccgccgatca tgcagtaacg
    21781 accaattttc aggctgcccg ccataatgac gccaccggca accgccgtat tgtcgccaat
    21841 cacgacgtta tgtgcaatct ggcactggtt atcaatgatc acgccattgc caataacagt
    21901 gtcatccagc gcgccgcgat cgatggtagt gcaggcaccg atctccacgc gatcgccaat
    21961 aattacgcga ccaatctgtg ggatcttcac ccagttacca cgatcgttgg cataaccaaa
    22021 gccgtctgcg cctaccactg ttccggactg gatcaggcaa ttctgaccga tctggatctc
    22081 atggtaaatg gttacgttcg cccagagacg cgaacctgca ccgattttgc tgtttttacc
    22141 tacgaagcaa ccggcaccga taatcacgtt atcgcccagt tcaacgccgg attcaatcac
    22201 cgcgttagcg ccaatcgata cgttgttacc cagcttcgcc gtcgcgtcga tcaccgcact
    22261 gggtgcaatg ttctgcgcgg gctgcggcgt ggtatctaaa atttgcgcca tgcgcgcgta
    22321 agtcaggtag ggattcttca ctaccagcgc ggcacttttc gcgaaaggaa gatcgtcctg
    22381 ggtcatgaca accgcggacg cctggcacaa gcctaaatgc tcacggtatt ttgggttaac
    22441 catgaacgta atgtgacctg tttgtgcaga ttgcatggac gcaacgccgg tgatgacgat
    22501 atcgccatca ccgtgtagtt ctgcatccaa ctgctgcgct aaatcagcca gtcgaattga
    22561 aggcattact tatttaacct gtttcagtac gtcggcagtg atgtctttta catcgctgct
    22621 gttgtaagca acggcgtttg catcaacaac cagatcgata tcctggctgt tggcaacgga
    22681 tttcacagca gtctggatac gagtaaccag tttgccgcgt tcttcattgg aacgacgtgc
    22741 gcgatcctgc tcaaaagcct gcgctttctg agcaaaagtc tggcgctgag ccatcacgtc
    22801 tttttccagc ttagtgcgat cgctgcccgc tttcatggac tgcagctttt tcattttagc
    22861 ctgcagatcg gtttccatac gctgcagttc gctggcacgg cctttgaact cattttccag
    22921 cgtgttagaa acaccggttt tctgcgctac ctgctggaac aggctgccca tgttgacgat
    22981 tgcaattttg tcagccgcct gagcagatgt tgccagtgct aaaccgagac ctgcagctaa
    23041 taaccacttt ttcacaataa actccttacc atcccatttg caccggaggg tgcagttctt
    23101 tgcgtggccc ggcgatctta tattgatcgc ctaaagtcat cgctacacta ccactacatt
    23161 cctttgtgga gaacacttac caggttttac cgatgttaaa ctggaactgt tctgccttgt
    23221 ctccatcgta ctttttgaac ggctgggcgt aggagaacac caacggcccc aatggggaca
    23281 tccattgtaa tgcgataccc gcagacatac ggatattgct tggatcacta tagtccggat
    23341 atccagaata ttggctggaa tcccagtttg tatcccaaac ggtacccata tcccagaaga
    23401 aggaagtacg aaccgagtta gcatacttat cgctaataaa cggcgtcggg gtgatgaact
    23461 cgaggctggc aaccgccatg gcgttaccgc ctacagcatc atccgattta cacaggtctt
    23521 tcgcgccgtc ctgagtcgca cattcgtaat catagtccgg atcataatta ctggcctgat
    23581 gcgggaagta aactgctttc ggaccaattg tattggactg gaagccacgc acggtgctgg
    23641 aaccaccggc atagaagttc tcgtagaacg gcatctcttt gccgcctaaa ccatcaccat
    23701 aaccccagcg ggtacgcccc agaacaaccc atttgtgatc gtcatcgatc ggcacataag
    23761 tcgccgtgtc taacgtcact ttgtagtatt cgttatccga tccaggaatg gtcactttac
    23821 cggtcaggtt gacacgtgaa ccatctgtcg ggaagtaacc acggtcaagc ttgttatagg
    23881 tccaaccata gttgaacgtg aagtcgtccg ttttgaagct gttatcctga tcagaggtgc
    23941 tcggatgttc acccatagag tacagataac gccacatcgc aacctgaggc tgcatgttgg
    24001 acagggagtt atgtacataa cccagacctg cacgcagcga gttatattcg ttaatcggga
    24061 agcccaacgt cacgtctgta ccataactct tgttggtata gtcggacagg tcggcgtcat
    24121 ctgcctggaa gtcattatag aagagacgac caccgaggct tacgccatct acggtgaagt
    24181 acgggttggt taccgacagt tcagcatagg tctggtaatc gtttttggtc ccgttgatac
    24241 caacagcata acctgtacct aaccagttat cctgttgcac gccagcctgg aagctcacgc
    24301 cactttcagt accgtaacca atcccgaagt tgaagctacc agtgttacgt tctttcacct
    24361 tgtagactac atctacctgg tccgggctac ccggaacacg ttgggtatcg gtatcgacag
    24421 tttcaaagaa gcccagacga ttcagacgct ccttaccctg atcgaccaga tcgcttccca
    24481 gccatgcacc ttccatctga cgcatttcgc gacgcaggac ggcatctttc gaggtatcgt
    24541 taccttcaaa acggatctta cgcacgtaga aacggttacc cgcatcaacg ttcacgcgta
    24601 atttaacggt tttgtcggca tcgttaattt caggcatcga ctgtacgcgc ggataggcat
    24661 aaccatagcg accgagaagc tttttaatgt catcttccat tttggtcact ttggtgccgt
    24721 tatacagctc gcccggctcg atcttagtca gctgctcaat ttcagcggag tgcccggcaa
    24781 ggttgccgct cacttcaacg ccagaaagct tgtactgatc gccttcggtg atgttcaccg
    24841 tgacgtaaat acctttttta tctggcgtca gactgacctg ggtagagtct atgttgaaac
    24901 gggcataacc gcggtccaga tagtagctgc gcagggtttc aaggtcgccc gccagtttct
    24961 gtttctggta tttacgatcg cctaccacgt tccaccacgg cacttcgtca cgcagttgga
    25021 aatgagagat cagttcgtcg gtggtgaaag catggttacc aacaatgtta atttgctgga
    25081 tttcagctga cacaccttcc tggaacacca gttttaggtc aacacggttg cgcggcagcg
    25141 gggtcacgac agctttaacg ctggcgctat atttaccgac gctgtagtag aagtcttcca
    25201 gacctttctc gatatcggca atggtggtgc gatcgaggga ttcgcccaca cgcacaccag
    25261 aagcctcgag gttttgcttc agcatgtcat ctttcaccga tttgttaccg gagaaagtaa
    25321 tgctggcaat ggtcggacgt tcttttacct gaaccagaag ggtatcacca tcacgaagga
    25381 cgcgaacatc ctcaaagttg ccggtagcaa acagagcgcg aatggtatta ctgatatctt
    25441 catcattaac cgtgtcgcct gtgcgcaccg gcatactgag gagggccgca ccaacggcga
    25501 cacgctgaag gccttcgaaa tgaatatctt tcactacgaa cccttcagca ccgtatacgg
    25561 tggcgctgct aaacagcagc gacgctatga gcaacttttt catcgccatc gttattatgc
    25621 gttcttccta actaactctc ataaccgaga gaaatcattg aaaagtgcaa gccccattaa
    25681 cagcaccagc agaatcgagc caatgcgata acaaaagtct tgaacccgct cggataccgg
    25741 tccgcctttg atcttttcga tcgcaaggaa cagcagatgc cccccgtcaa gtacgggcaa
    25801 cggaaacagg ttaattatcc ctaagttcac gctaataagc gcaagaaacg gcaggtaata
    25861 aactaccccg agttccgctg tcatcccagc ccccttggcg atagagatcg gcccactgag
    25921 gttgttcagt ttcacatcac cggtgatcaa ttttcccagc atactgaccg tcagcttcat
    25981 cagctgccac gttttgtccg tggcttcgac gatggcgttg aacggcccat actggcgtac
    26041 aactttatac tcatctggca aaggaatgac tttcggctca ataccgacaa aaccaatcgc
    26101 tttaccatta cccggtttac tctccgggat taatgtcaaa gacaagggac tcccctgcct
    26161 ttcgatttct aacgctaagg atttacccgg gttatcccgg acaagcatca caaaggtcac
    26221 ccactgcgtt aagggctgac catcgacttt aacgatcctg tcgcctgctt gcaaacctgc
    26281 cttgcttgcc gccgagtttg gctgcacatt ttccagtaca ggttcaattt gcggcccacg
    26341 aggacgaatc cccagcgaag ataccggatc ttctttatca ggctcaaacg cccagtgacg
    26401 taaatcgagc tttacatccc gccgttggtc gctgccaaat ggcgctactg taatggtggt
    26461 gctttcatcg ccaattttat cgaccaactg caaacgcacg gcatcccaat caggcgtttc
    26521 gataccatct acggctttta gttccgtacc tggtgcaatt tgtgcttccg cagctatcga
    26581 attggctgct atttcgccaa ccaccggacg tacgccaggc acaccaataa taaaaaccag
    26641 ccagtaggca aagatagcaa aaatgaagtt tgcaaccgga cctgcggcaa taatcgccgc
    26701 tcgttggccg acagatttat tattgaaggc atggtggcgg agttccggaa cgaccggttc
    26761 tgcgcgctca tccagcattt tgacataacc gcccaacggg atcagggcga taacatattc
    26821 ggtgccgagc ttatcagttc gccgccagag cgccttacca aaccctattg agaaacgctc
    26881 aacgcgaaca ccacaacgcc gggcaaccca gaaatgacca aattcatgca cggtgataag
    26941 tacacccagt gcaacgatga acgaagccaa atcccagaga aaactcagca tattaccttc
    27001 cgttaaagcg tcctgaatac cagtaacaac aagcaagcaa agaccggtac cgcagccgtc
    27061 aggctatcaa tacgatctaa aataccaccg tgtcctggaa ttaaatgacc gctgtcctta
    27121 attcctgctt cacgcttaaa catactctcg gtcagatcgc cgagcactga ggctaacgct
    27181 gcgacaatag agcaaatgag taaggtgacg ggagcgacgt cgagattcgc ccacatgcca
    27241 taaccccatg agattaccgc tgcagtagcg agtccaccga taaagccttg ccaggtttta
    27301 cccggagaaa ccttcggtgc cagcttatgt ttaccaaaca atttgccaaa catatatgcg
    27361 ccggagtcag cgccccatac caggatcatg acatagagca gccatattgc gccactgtaa
    27421 tgattctcgt catagtgcca ggcccgtaac gccagcatgc cccagaagaa gggaacaatg
    27481 gttagcacgc caaaaataag gcgcaatgtt ttagagttac gccagattgc tgcggaacct
    27541 gggtaaaaca gcaccagcaa tagcgcgaca atccaccaac ccagcgaagc ccaaagtgag
    27601 atttcaacca gcggttgatg aatatttcgg tgatattccg gcaacagaaa aagcatcagc
    27661 gccaacaata acccgcataa caccgccaac catactcgct gcgaacgagt ggtaaaaccg
    27721 ctaagctgtc cccattccca cgctgccagc atgcagacca ccagcgttac aatggcgaac
    27781 cccaccggcg gcaacagaaa caacgccgcg atgacgacgg gtattaacac aaaagcagat
    27841 atcaggcgat acttcagcaa aagcgacccc catcaggctg tttcatcacc gggctcggtg
    27901 ccgccgaaac gacgctctcg attagcaaag gcatttaacg ccccttcaaa gtcttgttca
    27961 tcgaaatcgg gccagagaac atctgtaaag taaagttcgg cataggcaat ttgccaaagc
    28021 aaaaagttac taatgcgatg ctccccccca gtcctaatta ctaaatctac aggggccagt
    28081 tcatgcatac agacatgctg gtttagcatc tcttcatcta tctgatctgg ttgcaggttt
    28141 ccttgctgca ccttttcagc cagttgcctg actccctgga ctatatccca acgtccaccg
    28201 tagttcgccg caatattcag cgtcagaccg gtattcccgg ctgttagcgc ttcagattta
    28261 cgaatacgtt cttgcaaacg cgagttaaag cgactggtat cgccaataat acgcagacgc
    28321 acgttatgtc ggtgcagact ttttacttcg ctatcgagcg cccacacaaa cagttccatt
    28381 aacgcactga cttcctgcgc tggtcggttc cagttttcac tactaaaggc atacagcgtt
    28441 aacgcctcaa taccgttgtt ggccgcaaaa gagacagccc ggcggacgga ttttgctccg
    28501 gctttatgcc caaaggcacg aatcttccct tgcttttttg cccagcgacc attgccgtcc
    28561 ataatgatcg caacatgacg gcagccatgc gctggcaatt tttcgctaag tggttgagta
    28621 gcagacaaca tcacgcgttt tttattccct gacagggttt acggtactca ggaatactga
    28681 agcctataca aaaaaaagcc gtgtaaaacc acggcatacc tgaccttata aagccaacta
    28741 cttacgatca gatggcgcag actatatcac tgaagcccta cgctaacaaa tagcgcgact
    28801 ctctgtagcc ggattatcct cagcttgcga gacgcatcac ctcttttctg gcgacttcac
    28861 gcgcgctcgc atcaacagat aacacatcgt ccacacattg tggttcgcgc atatccattt
    28921 tttccagtac ggataaattc aacgcagcga tatccgtaaa gcggatttgt tgcgcaagaa
    28981 aagcagcaac ggtgatttcg tttgcggcat tcaatgctgt cgtcgctgcc tggccttgtt
    29041 cgaacgcctc catcgccagt ttcaggcatg gataacgatc ataatccggt gcggcaaatg
    29101 tcaacgcact tagtttgcaa aaatcgagcg gcttcacgcc agagttcacg cgattcggcc
    29161 atgccatggt gtgggcaatt ggcgtacgca tatccggttc ccccagctgc gccagaacac
    29221 tgccgtcctg atagcgcacc attgagtgaa tcactgactg cgggtgaatc agcacttcca
    29281 tctggctggc gctggcgtta aacagccaac gcgcttcaat gtattccaga cctttgttca
    29341 tcatggtagc cgaatcgaca gaaattttac gtcccatcga ccagttcgga tgacggcagg
    29401 cttgatcggg cgtcattgtt gccaaatcgc gcaatggcgt ctcacggaaa gggccaccag
    29461 acccggtaag taaaatggac accacgccat tttgctcaag gtcagcgtat cccagattat
    29521 gctggatagg ttgcggtaaa ctctgaaaaa tggcgttatg ttcgctatcg accggtaaca
    29581 attgcgcttt gctctgcttt acggcgtcca taaacagacg tccgcaggta accagtgatt
    29641 ctttattggc cagcaaaatg gttttacccg cgcggatcgc agcaagcgta ggtaacagcc
    29701 cagcagcgcc aacaatggct gccatcacct gatcaacatc ctcaagcgct gccatatcgc
    29761 aagcggcttg ttgcccactt aagacttcgg tgcggctacc ctgttgctgt agcatcgttt
    29821 taagaagttt cgcactcgct tcatcgtcca ttacggcata gcggggagag aattccaggc
    29881 actgttctac catgcgagtg acatttttgc ctgccaccag cgcaactacg cggaagtgtt
    29941 cgggattatg gcgcaccacg tccagcgtgc tgcaaccaat cgagccggtc gagcccagaa
    30001 tggtgagttg cttcatgaaa catccagagt tgagacagaa taaaaagcaa aacgccgcca
    30061 gccgatccgc aaggatctac tgagcggcgt ttttgtcgtt caagaaatca gaactgcatc
    30121 agttctgctt ctttgtctgc cagcgccgct tcaattttct tgattgcagc atcagtcagt
    30181 ttctgtacat cgtcctgaga acggcgatcg tcgtcttcgc tgatctcttt atctttcaac
    30241 agtgctttca ctttgtcgtt cgcgtcacga cgcacgttac gtactgcaac acgcgcttgt
    30301 tctgcttcac cacgaacgat tttggtcaga tctttacgac gttcttccgt cagcggcggc
    30361 agcggaacac ggatgtcgct acccgcagag ttcgggttca ggccaagatc ggacgccata
    30421 atcgcttttt caacggccgg agacattgaa cgatcaaaca cgttgatttt cagagtacgg
    30481 gaatcttcta ccgttacgct tgccagctga cgcagcggcg tcggcgtgcc gtaatattcc
    30541 acgacaatgc catccagcag gctgggagaa gcacgacccg tgcgtatttt gctgatttgg
    30601 gttttgaacg cttctacgca tttgtccatg cgtacttcag catcttttct gatatcgcta
    30661 atcacgttac gaatccttga aaacttgtct cagtcagacc agacagtcac acacagtgga
    30721 agtgtgctaa gtatagccct gattaaacat attacgggta agatcccccg cgatacttac
    30781 gcggaatctt acccttattt atccatcacg ggaattattc cgtgattaaa gtcccttctt
    30841 tttcacccat taccacacgg cgcagcgcac ccggtttgtt catgttgaaa acacgaatcg
    30901 gtaatttatg gtcacgagcc agcgtgaagg ccgccaggtc catgactttc agctcttttt
    30961 ccagcacttc gctgtaagtc agttgctcgt acatggttgc ggttggatct ttcgccggat
    31021 cagcggtaaa cacgccgtca actttggttg ctttcagcac cacatcggct tcaatttcga
    31081 taccacgcag gcaagctgct gagtcggtgg taaagaacgg gttacctgta ccggcggaga
    31141 ggatcaccac acggttgttg cgcaacaggc tgatagcttc tgcccagctg tagctgtcgc
    31201 acacgccatt caatggaata gcggacatca gacgagcgtt cacataggcg cggtgcagtg
    31261 catcacgcat tgccaggccg ttcattacgg tcgccagcat ccccatgtgg tcgcccacaa
    31321 cgcggttcat acccgctttc gccagaccag cgccacggaa caggttaccc ccaccaatca
    31381 ccacaccaac ctgaataccc agttcaacca gttctttgat ttcctgagcc atacgatcca
    31441 gtatgcttgc atcaataccg aagccttcag tgccctgcag agcttcgcca ctcaacttaa
    31501 gcagaatgcg tttatagacg ggttttgcat tggtagccat gtttctttcc tgagactgtc
    31561 aacgattgaa atgaattaat actagcgaca ttgtacgtcg cttttcagcg cagccccaca
    31621 agggttagct gaatttacaa gatgggcaca aaaagaagcc gccctcaggc ggctcctttt
    31681 tgataattaa gactgcttgg acatcgcagc aacttctgct gcaaagtcag tctcaacttt
    31741 ctcgatgcct tcacccactt cgaagcggat gaagccagtc acttcagcgt tatgctcttt
    31801 cagcagctga ccaacagttt tgcttggttc cataacgaac ggctgaccgg tcagagaaac
    31861 ttcgccggtg aatttcttca tgcggccttc aaccattttc tctgcgattt ctttcggctt
    31921 accagactgc atcgcgatat ccagctgtac ctggtattct ttttctacca cttcagcgga
    31981 tacgtcttcc ggtttgatga attctggctt gcttgcagca acgtgcatag cgatgtgttt
    32041 aaccagctct tcgtcagcgc ctttagcagc aaccagaacg ccgatacgcg caccatgctg
    32101 ataagaaccc agaacgtcgc cttccagcgc agcaacgcgg cgaatgttga tgttttcacc
    32161 aattttcgct accagcgcaa cacgttcttc ttcgaactgt gctttcagaa cttcaacgtc
    32221 agtgattttg ccagcaacag ctgcgtccag aactttgtct gcgaacgcct ggaaaccagc
    32281 gtcttttgca acgaagtcag tctggcagtt aacttccaga atgatgccgt agttgccgtc
    32341 gattttggtt ttgatcacgc cgtcagcagc aacgttgcct gcttttttcg ctgctttaat
    32401 agcaccggac ttacgcatgt tttcgattgc cagctcgatg tcgccgttag cttcagtcag
    32461 tgcttttttg caatccatca tgcctgcgcc agtacgctca cgcagctctt ttaccaggga
    32521 tgcggtaatt tcagccattc taaaatcctc ggagatgtga tctgcccggc attaaaccgc
    32581 acagatataa aagtgaaaaa gggggccata tgcaggcccc ctaaccaaac gtgtactacc
    32641 tggtctataa gggctctccc ccttcatatt tcgcgccgca gatgcgtcat cttcgctcgc
    32701 tcatcccggt cacttactga tgtaagctcc cgggaattct cgagcttgcc gcctttctgc
    32761 aactcgaact attttggggg agttatcaag ccttattact cagcttctac gaagctttct
    32821 tccgcctggg aagccagatc ctgagaacgg ccttcacgta cggttgcagc aacagcgccc
    32881 aggtacaggg tcacagcacg gattgcgtcg tcgttacccg ggataacgaa gtcaacaccg
    32941 tccggatcag agttggtatc aacgatagca aataccggaa tacccaggtt gtttgcttct
    33001 ttgatagcaa tgtgttcgtg gtcagcatcg attacaaaca gagcgtccgg cagaccgccc
    33061 atgtctttga taccgcccag gctgttttcc agtttctcca gctcacgagt gcgcatcagc
    33121 gcttctttct tggtcagctt gtcgaaagta ccgtcctgag actgagtttc caggtctttc
    33181 agacgtttga tggactgacg aacggttttc cagttagtca gcataccgcc cagccagcga
    33241 tggttcacga agaactggtc gcagctcaga gcagcgtctt tcaccgcttc gcttgcagcg
    33301 cgtttagtac caacgaaaag gattttacct ttgcgagaag caatcttgtt cagttcagcc
    33361 agagcttcgt tgaacatcgg tacagttttc tcaaggttga tgatgtgaac tttgttacgc
    33421 gcaccgaaga tgaacggctt cattttcggg ttccagtaac gggtctggtg accgaagtga
    33481 acaccagcct tgagcatgtc gcgcatggaa acagttgcca tgattaaaac ctctatataa
    33541 aagttggggt tatgcctcca cgtatcccat attaccgacc ccaaagggca ccccggaata
    33601 tgtgccgata cgtgtgtgtt gttacacaaa gtgagattgt cgcttccgtc cagtctgtgt
    33661 atacgaaatg gatcggaagt ccggcgcgct ttataccaca aatacgccgt ggacaccaat
    33721 aattgttggc gctgtgtaca gcatcagacg tcgaattttc tattatagaa aaccttcagt
    33781 ggcacgtttg gcgaaattca gaatgattct caatttgccc gggtgtgata ccattgacgg
    33841 cacttacata tatattgtcg gtatcaccga cgctgatgga cagaattaat ggctatctca
    33901 atcaagaccc cagaagatat cgaaaaaatg cgcgtcgctg gccgactggc tgccgaagtg
    33961 ctggagatga tcgaaccata tgttaaaccg ggcgtcagca ccggcgagct ggatcgcatc
    34021 tgtaatgatt acattgttaa tgaacaacac gcggtttcag cctgcctcgg ctatcacggt
    34081 tatccgaaat ccgtttgcat ttctattaat gaagtggtgt gccacggtat tccggacgat
    34141 gctaagctgc tgaaagatgg cgatatcgtt aacattgatg tcaccgtaat caaagatggt
    34201 ttccacggcg atacctcgaa aatgtttatc gtcggtaagc cgaccatcat gggcgaacgt
    34261 ctgtgccgta tcacgcaaga aagcctgtac ctggcgctac gcatggtaaa accaggcatt
    34321 aatctgcgcg aaatcggtgc ggcgattcag aaatttgtcg aagcagaagg cttctccgtc
    34381 gttcgtgaat attgcggaca cggtattggt cgcggcttcc atgaagaacc gcaggtgctg
    34441 cactatgact cccgtgaaac caacgtcgta ctgaaacctg ggatgacgtt caccatcgag
    34501 ccaatggtca acgcgggtaa aaaagagatc cgcaccatga aagatggctg gacggtaaaa
    34561 accaaagatc gcagcttgtc tgcacaatat gagcatacta ttgtggtgac tgataacggc
    34621 tgcgaaattc tgacgctacg caaggatgac accatcccgg cgataatctc gcacgacgaa
    34681 taaaatgaag ccggcgaatg ccggcttttt taatgcgata atttaatctt atgggtggcg
    34741 cacaatgaat acccttccag aacagtacgc aaacaccgct ctccccaccc tgcccggtca
    34801 accgcaaaat ccatgcgtct ggccccgtga tgaattaacc gtcggtggga taaaagccca
    34861 tatcgatact ttccagcgtt ggctgggtga tgcctttgac aatgggatct ctgcagaaca
    34921 gttgattgag gcgcgcaccg agtttatcga ccagctcctg caacgattat ggattgaagc
    34981 gggattcagc cagattgccg acctggcatt ggtcgccgtc ggtggctacg gtcgtggcga
    35041 gctgcatcca ctttctgaca tcgatttact gattttaagc cgtaaaaagc tcccggacga
    35101 tcaggcgcaa aaagtgggcg agctgttaac gctgctctgg gatgtaaagc tggaagtcgg
    35161 tcatagtgtg cgcacgcttg aagagtgcat gctggaaggg ttatcggatt taaccgtcgc
    35221 caccaatttg atcgaatccc gcttattaat tggcgatgtt gcgctatttc ttgaactgca
    35281 aaaacatatt ttcagcgaag gattctggcc ttccgacaag ttctacgcgg cgaaagttga
    35341 agaacagaac cagcgccatc agcgttacca cggcaccagc tacaatcttg aaccagacat
    35401 caaaagtagc cctggcggct tgcgcgatat ccacactctg caatgggtgg cccgccgtca
    35461 ttttggcgca acatcgctgg atgaaatggt tgggtttggc ttcttaacct cagcggagcg
    35521 ggcggaatta aacgaatgtc tgcatatatt gtggcgtatt cgctttgccc tgcatctggt
    35581 cgtcagccgt tacgataatc gcctgttatt cgatcgccag cttagcgtcg cccagcgtct
    35641 gaattacagt ggcgaaggta acgaaccggt cgagcggatg atgaaggatt acttccgcgt
    35701 gacacgccgc gtcagtgaac tcaaccagat gctgctgcaa ctgttcgatg aagccatcct
    35761 cgcccttccc gccgacgaaa aaccacgtcc aatcgacgat gagtttcagc tacgcggtac
    35821 gctaatcgac ctgcgtgatg aaacactatt tatgcgccag ccggaagcca tcttgcgtat
    35881 gttctacacc atggtgcgca acagtgcgat caccggcatt tactccacca cactgcgcca
    35941 gttacgccat gcccgtcgcc atctgcaaca accgctgtgt aatattccgc aagcacgaaa
    36001 actgtttttg agcattctac gtcaccccgg agcggtgcgg cgcgggctat tgccaatgca
    36061 tcgccatagc gtactcggcg cgtatatgcc gcaatggtca catatcgtcg ggcagatgca
    36121 gtttgatctg ttccacgcct acacggtgga tgaacatact atccgcgtga tgctgaaact
    36181 ggagagtttt gccagtgaag aaacgcgcca gcgccatccg ttgtgtgtga acgtctggcc
    36241 gcgcctaccg tcaactgagc tgattttcat cgccgcgctg tttcacgata tcgccaaagg
    36301 acgcggcggc gaccactcta ttctcggtgc tcaggatgta gtgcattttg ccgaactcca
    36361 cgggctgaac tcacgcgaaa cacagctggt cgcctggctg gttcgccagc acttgctgat
    36421 gtcggtgaca gctcagcgtc gcgatattca ggacccggaa gtcatcaagc agtttgccga
    36481 agaagttcaa acggaaaatc gtctgcgcta tctggtatgc ctgacagtgg ctgacatttg
    36541 cgccaccaac gaaacgctgt ggaatagctg gaagcaaagt ctgttgcgcg aactctattt
    36601 cgccaccgaa aagcagctac gacgcggaat gcaaaacacg ccggatatgc gcgaacgggt
    36661 tcgccatcac caactccagg cgctggcact actgcgcatg gataacatcg acgaagaggt
    36721 gctgcaccaa atttggtcac gctgtcgcgc gaactatttt gtccgtcata gcccaaatca
    36781 actggcctgg catgcgcgcc atttattaca gcatgattta agcaaaccgc tggtattgct
    36841 tagcccgcag gctacgcgtg gaggcaccga gatttttatc tggagcccgg accgccctta
    36901 cttgtttgct gctgtttgtg ccgagttaga tagacgcaac ttaagcgttc acgacgcaca
    36961 aattttcacc actcgcgacg gtatggcgat ggataccttt atcgtgctgg aacccgatgg
    37021 tagcccgcta tccgcagatc gtcatgaggt tattcggttt ggtctggagc aagtactgac
    37081 acaaagtagc tggcagccac cacagccgcg tcgtcaaccg gctaaactac gtcactttac
    37141 tgttgaaacc gaagtgacgt ttttgccaac ccataccgac cgcaaatcgt tccttgagct
    37201 tatcgcgctc gaccagccag gactgctggc gagagtcggg aaaatttttg ccgatctggg
    37261 aatttcgctt catggtgccc gaattacaac cattggcgag cgagtagaag atttattcat
    37321 aattgccacc gctgaccggc gtgcacttaa taacgagttg cagcaggaag tgcatcagcg
    37381 gttgacagag gccctcaatc caaacgataa agggtgatgt gtttactgat atgaaaagag
    37441 tttaacaatg cagcagttac agaacattat tgaaaccgct tttgaacgcc gtgccgagat
    37501 cacgccagcc aatgcagaca ccgttacccg cgaagcggta aatcaggtga tcgccctgct
    37561 ggattccggc gcactgcgtg tagcggaaaa aattgacggt cagtgggtga cgcatcagtg
    37621 gttgaaaaaa gcggtgctgc tctctttccg tattaatgat aatcaggtga tcgaaggggc
    37681 agaaagccgc tacttcgaca aagtgccgat gaaattcgcc gactacgacg aagcacgttt
    37741 ccagaaagaa ggcttccgcg ttgtaccacc tgcggcggta cgtcagggtg cgtttattgc
    37801 ccgtaacacc gtgctgatgc cgtcttacgt caacatcggc gcatatgttg atgaaggcac
    37861 catggtcgat acctgggcga ccgtcggttc ttgtgcgcag attggtaaaa acgtccacct
    37921 ttccggtggc gtgggcatcg gcggtgtgct ggaaccgctg caggctaacc caaccatcat
    37981 tgaagataat tgcttcatcg gcgcgcgctc tgaagtagtt gaaggggtga ttgtcgaaga
    38041 aggttccgtc atttccatgg gcgtatacat tggtcagagc acccgtattt acgaccgtga
    38101 aaccggcgac atccactacg gtcgcgttcc ggcggggtct gtggttgttt caggtaatct
    38161 gccgtcgaaa gatggcaaat acagcctcta ctgtgcggtt atcgttaaga aagttgacgc
    38221 gaaaactcgc ggcaaagtcg gcattaacga actgctgcgt accatcgact aaaagtatgc
    38281 acacgggcag cacgacgctg cccgattgct ttctggttca gaataattcg tcctactcat
    38341 tcttcctccc ccctttttta aatacttttt catcttgttt cggactgtca tgtaattcgc
    38401 aaaacgaaga taaatgtgct aatccatccc atggaatatc gccacaataa agcaggtaat
    38461 tttttgattt ttacgccatg atagaagtct ggaagaaaaa ggctttctgc gatcacagtc
    38521 gagccgggcg ggccaggcca aatgctaaaa taagcacatt ttactgactc attttgaggt
    38581 aacgctatgt acgacaatct gaaaagtctg ggtattacca atcctgaaga aattgatcgt
    38641 tacagcctcc ggcaggaagc caacaacgat attctgaaaa tctatttcca gaaagacaaa
    38701 ggcgagtttt tcgccaagag cgttaagttt aaatatccgc gtcagcgtaa aacggtcgtc
    38761 gctgatggtg tgggtcaggg ttataaagaa gtccaggaaa tcagcccgaa tctacggtat
    38821 atcattgatg agcttgatca aatctgccag cgtgaccgca gcgaagttga tcttaagcgt
    38881 aagatcctcg acgacttacg tcacctggag tcagtcgtaa ccaataagat cagcgagatt
    38941 gaagccgatc tggaaaaact aacgcgtaaa taatcccgaa ttgacggtta actcgggcat
    39001 tcccctctcc ccttcgggct gaggggaatg agccagcgcc gcctcaacct taacgcataa
    39061 cctaccgctc ttcatccagt tgtaacgcca catacagcag caacctgtca tcaaaattgc
    39121 ccaaatcaag cccggtcagt tccgatatac gattaagccg atactccagg gtattacgat
    39181 gaataaacaa cgcctttgac gttgccagcg gttgcacatt gtgacgaaac cacgccgcca
    39241 gcgttcgtcg cagcaagccg ttattgtcca tcgctttcag ccgcgccagc ggtcgcgcca
    39301 gttcgttggc ctgccagtcg ccacgcaaac tgtcgagtaa cacaggtaac atcagatcct
    39361 gataaaaata gcagcgactt tctggcatcc gctgtttacc caccaccatc gtcgttttcg
    39421 ccgtacgata ggatcgggca atactgccag gaccggtaaa atagttgccc agtgaaacgc
    39481 gaaaacgcag ctggccgtac tctttcatgc gggtaatcag ttgttcaact cgcttacgat
    39541 gatcttctac atcccagcgc ccaaaagagt tcaacgccgg tttcaacacc accatttcgg
    39601 ttagcgagac aatcgccacc agattattac gctcgggcgt agtcagcgcg ttttgcagtt
    39661 gttgtaactc cgccattgcg ctgtccacac caagctgacc gctgtcgacc tcaacaatag
    39721 ccaccactcg cggttgattg agatcgatcc ccagccgttg cgcccattca gtaagtgcgg
    39781 gagtattctc ctctgcctga atcaggttca tcaccagttc ctcccgcaaa cggctatcct
    39841 gcgccaacaa gtgcatcaac cgcgactgtt ccagcatcat ttcagccgtc atgcagacca
    39901 gttcgccata tttacgcaga ttctctggtt cacctgtcag gccaattacg ccgacaattt
    39961 caccttccag ccgtaacggt agattaatcc cttgccgcac accgtgcaga tgacgtgcca
    40021 ccgcgtcatc gatatcgacg actcgtccct gtgaaagtac cagcaatgca ccttcgtgca
    40081 attcaccaat acgctcacga tcgccgctgc cgataattcg cccacgggca tccattacgt
    40141 tgatattggt atcgatgatg cgcatggtac gtgccacgat atcctgcgcc attttggtat
    40201 caagatgcca gccagccata agtcctccgt tatgcacggc ttagcataag gaagtacgta
    40261 acgtacggca ttgtgcaaat gcctaaagga tgatgaagat gtatggagtt gtggtgaagt
    40321 tcacagattg taaggagacc cccttcccgt tttcaggaag gggttgaggg agattactgc
    40381 attaacaggt agatggtgct gtcgccgcgc tgaatgttca gtgccagcac agacggtttg
    40441 ctgtcgagaa ctttacgcag ttcagcgatg tttttcacag cctgctggtt cgcgccaata
    40501 atcacatcac ctttcttcag gccgatctgc gcagccggag tgcccgtttt cacgttgttc
    40561 actaccacgc cctgatcttt gcctttgttg ctcatctcag cgccttcaat gccgttgaag
    40621 atggagctgg aatcaacctg attctggctg ctctgctgca gttccagatt cacgttgacc
    40681 tgcttaccgt cgcgcagcaa gcccagggtc agtttgctgc ccaccggcat agtacccact
    40741 tgagcacgca gtgcggcaaa gctgctgatc ggcttaccgt tcagtgaggt gatcacatca
    40801 cccgctttaa tgcccgcttt tgcagcggag gaattaggca gaacctggct tacgaaagca
    40861 ccgcgctggg cgtcaacttt catcgctttc gccagttcgg agttcagctc agtccccata
    40921 atacccagct caccgcgttt cacctggccg tattccacca tctgcgaggt caggtttttc
    40981 accatgttac tcgggatagc aaaaccgata ccgatgttac cgccgtccgg tgctaggatc
    41041 gcggtgttga taccgatcag ttcgccgttc aggttaacca gcgcaccacc ggagttacca
    41101 cggttgatcg ctgcatcggt ctggatgaag ttttcgtagt tttcggcatt caggccgcta
    41161 cgccccagcg cagagacaat cccggaagtt accgtctcgc ccagaccaaa cgggttacca
    41221 atcgctacgg tgtaatcacc cacgcgcagt gcatcagaat ccgccatctt aattgcggtc
    41281 aggtttttcg gattctggat ttggatcagc gcgatatcag agcgcggatc tttgccaacc
    41341 atcttcgcgt cgaacttacg gccatcgctc agttgaactt taatgaccgt cgcgttatca
    41401 acaacgtggt tgttggtgac gacatagcct ttatcggcat caatgatgac gccggaaccc
    41461 agcgccatga atttctgttg ctggccgcca ccattaccgc cctggccacc ctggcagaac
    41521 ggagagctct ggaacggaga accttcctgg cagaacggag aatcatcacc gaagaactgc
    41581 tggaaattac gcggcatacg cggcgtatta acggttgtgc taccttctac gttaatgctg
    41641 accactgaag gcatcacctt ttcgagcatc ggtgcaaggc ttggcatctg ctgggctgtc
    41701 gttgctgaag aagtctcagc cgccgttgca gagagcggag ataacgccaa acctaaactc
    41761 agagccagtg cactcagtgc taatgtggtt tttttcatgt atttcagtct cgattaacag
    41821 ataacgcaaa attgctgtgt tcttcagatt cgttttatag cgcgaagttc cgaactaaag
    41881 tttctgcaaa aggtaaaaat ttattgttcg tctttacaaa agcctggtta ttgttctacg
    41941 gccatcagac gtcggtattc atcccaggca tagaggtcgg tcataccgct gatataatcc
    42001 tgcagcaggc ggcaacggta ataatattcc catagcggaa actcaggaga atctgacggt
    42061 aatttactga cagcctcgac ataggccagc cgatggcgcg tcgagagttt gtggaataag
    42121 cgagattcaa taggggaacg tttcacccgt tctttttcta ccagttcagt aaagtctgat
    42181 aatgacaggc ttaataaagg gcgataaatc tctaataatc cgctaatgac ccgatagccc
    42241 tgcaattcaa gctgctcgac atctggatgg ctaaacacat gttttacagc gacattttta
    42301 tatagcttaa gaagatcgct gcattcgctg gcatcttcca ataatgcatg attaaacgtt
    42361 ccggcgaaaa tcgcaggcag attatcaata aatcgttgtg ccgcgtaggg taccagttta
    42421 tttagggtgt ttacccgtaa atacataaaa aactgatctt ccgtactgcg gcttaaacta
    42481 tttgagcgtg atttttccca ggcattttca accaccagcg aaaagagcga acctttctca
    42541 tgctggcccc acgcctcgtg caaatgatga taaagctgct caacggtaaa tattcttttc
    42601 tctaccgcat cttcaagatc tgccacacaa taggagatgt cgtcggcagc ttccataatc
    42661 cacgttaatg gaaaacgact gtaaagcgcc aaattaagtt ctttacgcaa ccgggcaata
    42721 taggcttctt cagaaagata ataacccggc tttttcatta aatagtgatg tgtctcaggc
    42781 gtttcgccac gccaccacgc tggacgggta tattttaaaa taccgccaac ctgcgcccag
    42841 gtgagattca tccgcatcaa tgtatgcacc agacgaatgc cttgtgcatt cccctcaaaa
    42901 tgacataagt cctgacgaat cttgcgccgc agctcgttaa gcggttcttc cccgtcccgt
    42961 aaacgtagta ccgccacgct gcagcgatcg tcagtcagag gctggctttc ggcatcttcc
    43021 gggtgcaaac gctggcgaaa ccagtcattt atcgccgctt cgccaaaatg accaaacggc
    43081 ggattgccga tatcgtgcat caggcatgac atctcaacaa tgctttcaaa aggtccggtc
    43141 agttcatcca ggccgtatgc ttccagcaat ttaagctctt tcagacggct taaaatttct
    43201 ttggcgatgt agcgccccac ctgctggact tccatcgagt gggtaagacg cgtgcgcacg
    43261 gcggcattgc gctccagtgg aaaaacctgg gtcttttgtt gcagacgacg aattgccgga
    43321 gagttgatga tacgcccgcg atcgctctcg aagatccgca ggatctcatg ttcggtttta
    43381 acgccctgcg gtgaacggta acgacgatgc cagtttattt tttttcggaa atcaatctgt
    43441 gccatgtcct ctcccgcgtg agaaatacgc ttccccgtaa gcgcatggta aactatgcct
    43501 tcaaatcggg cttatcgcga gtaaatctat gaaaatcggc atcattggtg caatggaaga
    43561 agaagttacg ctgctgcgtg acaaaatcga aaaccgtcaa actatcagtc tgggcggttg
    43621 cgaaatctat accggccaac tgaatggaac cgaggttgcg cttctgaaat cgggcatcgg
    43681 taaagtcgct gcggcgctgg gtgccactct gctgttggaa cactgcaagc cagatgtgat
    43741 tattaacacc ggttctgccg gtggcctggc accaacgctg aaagtgggcg atatcgttgt
    43801 ctcggacgaa gcgcgttatc acgacgcgga tgtcacggca tttggttatg aatacggtca
    43861 gttaccaggc tgtccggcag gctttaaagc tgacgataaa ctgatcgctg ccgctgaggc
    43921 ctgcattgcc gaactgaatc ttaacgctgt acgtggcctg attgttagcg gcgacgcttt
    43981 catcaacggt tctgttggtc tggcgaaaat ccgccacaac ttcccacagg ccattgctgt
    44041 agagatggaa gcgacggcaa tcgcccatgt ctgccacaat ttcaacgtcc cgtttgttgt
    44101 cgtacgcgcc atctccgacg tggccgatca acagtctcat cttagcttcg atgagttcct
    44161 ggctgttgcc gctaaacagt ccagcctgat ggttgagtca ctagtgcaga aactggcaca
    44221 tggctaagtc actgttcagg gcgctggtcg ccctgtcttt tcttgcgcca ctgtggctca
    44281 acgccgcgcc gcgcgtcatc acgctttctc ccgccaacac tgaacttgcc tttgccgccg
    44341 ggatcacgcc ggttggggtc agcagctatt ccgactatcc tccacaagcg caaaagattg
    44401 agcaggtttc cacctggcag gggatgaatc tggaacgcat tgtcgcgctg aaacccgatc
    44461 tggtgattgc ctggcgtgga ggtaatgccg agcggcaggt tgaccagctg gcttcgctgg
    44521 gaataaaagt gatgtgggtc gatgcgacaa acattgaaca aattgccaat gcgttacgtc
    44581 aactggctcc ctggagtccg caaccggata aggccgaaca agccgcgcaa tccctgctgg
    44641 atcaatacgc gcaattgaaa gcgcaatatg ctgataaacc taaaaaacgt gtttttctgc
    44701 aattcggcat taatccgcca tttaccagtg gaaaagagtc gattcaaaac caggtactcg
    44761 aagtttgtgg cggagaaaac atctttaaag acagccgggt tccctggccg caagttagcc
    44821 gcgaacaggt gttagcacgc tcgccacagg cgattgtcat tacaggcgga ccggaccaaa
    44881 ttcctaaaat caaacaatac tggggtgaac agctcaaaat tcccgttatt cctctcacga
    44941 gtgactggtt tgaacgtgca agcccacgta ttatcctcgc tgcacaacag ctctgtaatg
    45001 cgctttcaca ggtagattag cgcctgacct ttttaggtgc tcagtgggga ttgaacaatg
    45061 ctcgtctatt ggctggatat agtcggcaca gcggtatttg ccatctccgg cgttttgtta
    45121 gccggaaaat tgcgtatgga cccttttggt gttctggtac tgggcgtggt taccgctgta
    45181 ggcggcggga caattcgcga catggcgctg gatcacggcc cggtattttg ggtgaaagat
    45241 cccaccgatc tggtcgttgc aatggtaacc agcatgctga ccatcgtgct ggtgcgccag
    45301 ccaagacgct taccaaaatg gatgttgccg gtgctggacg ccgttggtct ggcggtgttt
    45361 gtcggcattg gcgtgaataa agcctttaat gcggaagccg gtccgttaat cgcggtttgt
    45421 atgggcgtca ttactggcgt tggcggcggg atcattcgtg atgttctggc ccgcgaaatc
    45481 cccatgattt tacgtacaga aatctacgca actgcctgta ttatcggcgg tattgtccac
    45541 gctacggctt attacacatt ttccgtacca ctggaaacag ccagtatgat gggcatggtc
    45601 gtgacgctat tgattcggct ggcggctatt cgttggcatc ttaagctacc gacgtttgcg
    45661 ctggatgaga atgggcgttg aagagagaag atgtgccgga tagtttatcc ggcacatgaa
    45721 caacagatta gatactaaag gaagaaccgc aaccgcaggt gcttttcgcg ttcgggttgg
    45781 tcacgatgaa acgagaacct tccagtcctt cggtatagtc aacggaaccg ccgaccagat
    45841 attgcaggct catcggatca accaccaggc caacgccctg tttttcgatg gtcatatcgc
    45901 cttcgttcac ctgatcatca aaggtgaaac catactggaa gccgctgcaa ccgccaccgg
    45961 tgatatacac gcgtaatttc agattcgggt tatcttcgtc agcgatcagg cttttaactt
    46021 tgttggctgc tgcgtcggta aactccagcg gcagtgctac gtcatcactc atattttgct
    46081 ccaaacgaca tcggcaattg ggcaaattct aacccaataa taatcgccat tatctaatac
    46141 cctggtattt cgttcaagta ttctcgctgg ctgatgcggc cttgcttcgc gccagttgct
    46201 cagcttcctg ttttgccagc gtgcgcgcaa gaatcgccga gtatagcggt ttcccgccgg
    46261 taaattgcgc taatagtgtt gcgccaagac cggtaataat cattggcaaa atgagctggt
    46321 agttatcggt catctccaga accagaatga tccccgttaa cggcgcgcga atagatgccg
    46381 ccagtaacgc ccccattccg gcaatagcaa acgtccccgc ctcaaggtga tattgcggaa
    46441 acagctcaac ggcaaccatt ccgaaagcgg ttcccagcac agtacccagc gccagcatcg
    46501 gggcaaaaat accgcccggc gcgccggaag agaagcagag taaggtggta atgacccgcg
    46561 cgacgaagat aaacaccagc attcccatgc tgaaattccc cgcggtagcg ataggaatca
    46621 ggttaaaacc gccgcccgac gttgctggtg ccacaaaccc cagcaatcca cacagaccgc
    46681 caatcgcacc acccatcagt acccatttgg taatattgcc gccgtgcaca cggtgcagca
    46741 aatcctgcat ccccagcacc catttattaa aaatagggcc gaaaatgcca aaaataatac
    46801 cgaggatcag ataaagccac agcgtattaa gcggcgcgtc agaaagttta ccgacgtcaa
    46861 tcaacgcaac ttcatgatta aaaatccggt acataatggt cgacataatg acgccaataa
    46921 ataccgcttt aatcgaaatt aacgtatagc gaaactgcgg acgcatctct tcgataataa
    46981 acaaaatacc cgccagcggc gcgttaaagg ccgccgccag acccgccgcc gcaccggttg
    47041 ccaacagcgt atggcgagct tcatcacctt tcaggcggaa aacatcgagt accatccgcc
    47101 cgatattacc gccgatctgc acggttggcc cttcgcgccc caacaccatg cctccgccga
    47161 gtgtccccag cccgccaaag aatttcaccg gcaatacacg ccaccagcga acaggacgtt
    47221 gatcttccag cgccccttca atttccggga tccccgaccc ccctgcttcg ggcgcgtatt
    47281 tgcgcaccaa aaagtagccg aacatcgcca gcaccgccga acagagaaaa gcaacggtta
    47341 acagtagcgg ataattatca gcagtatgta ccagcgcccc catacgttgg ttctgcaacc
    47401 aggcgacacc tttgtcaaaa gcaaccgctg ccagcccgac aagcgtgccg acgactgccg
    47461 ccataaacaa aatggctaac ggggttttat cgcgctcaag aagttggcga atcagttgtc
    47521 tgcgtcgcag gcgcgcggcc tgcggtgttt ctaaagaggg agtatcagtt ttcatccaat
    47581 gatcacttat tggtcataca aataagatga cggcatttta ctcgcacgtt ccatgaaaat
    47641 cctttgcaaa gcgtaatgtt tcaaatgcac aaaactttta tactctaaga aatcaggatt
    47701 gcaccagtac aagcagcctg atgtttgacg agtatttaac ttgttatgaa taacatagaa
    47761 tagcagccat tcactttttc tacgaatcag gaaccctcca tgagtaagtc tgaaaatctt
    47821 tacagcgcag cgcgcgagct gatccctggc ggtgtgaact cccctgttcg cgcctttact
    47881 ggcgtgggcg gcactccact gtttatcgaa aaagcggacg gcgcttatct gtacgatgtt
    47941 gatggcaaag cctatatcga ttatgtcggt tcctgggggc cgatggtgct gggccataac
    48001 catccggcga tccgcaatgc cgtgattgaa gccgccgagc gtggtttaag ctttggtgca
    48061 ccaaccgaaa tggaagtgaa aatggcgcaa ctggtgaccg aactggtccc gaccatggat
    48121 atggtgcgca tggtgaactc cggcactgaa gcgacaatga gcgccatccg cctggcccgt
    48181 ggttttaccg gtcgcgacaa aattattaaa tttgaaggtt gttaccacgg tcacgctgac
    48241 tgcctgctgg tgaaagccgg ctctggcgca ctcacgttag gccagccaaa ctcgccgggc
    48301 gttccggcag atttcgccaa acatacctta acctgtactt ataacgatct ggcttctgta
    48361 cgcgccgcgt ttgagcaata cccgcaagag attgcctgta ttatcgtcga gccggtggca
    48421 ggcaatatga actgtgttcc accgctgcca gacttcctgc caggtctgcg cgcgctgtgc
    48481 gacgaatttg gcgcgttgct gatcatcgat gaagtaatga ccggtttccg cgtagcgcta
    48541 gctggcgcac aggattatta cggtgtggaa ccggatctca cttgcctggg caaaatcatc
    48601 ggcggtggaa tgccggtagg cgcattcggt ggtcgtcgtg atgtaatgga tgcgctggcc
    48661 ccgacgggtc cggtctatca ggcgggtacg ctttccggta acccgattgc gatggcagcc
    48721 ggtttcgcct gtctgaatga agtcgcgcag ccgggcgttc acgaaacgct ggatgagctg
    48781 acaacacgtc tggcagaagg tctgctggaa gcggcagaag aagccggaat tccgctggtg
    48841 gtaaaccacg ttggcggcat gttcggtatt ttctttaccg acgccgagtc cgtgacgtgc
    48901 tatcaggatg tgatggcctg tgacgtcgag cgttttaagc gtttcttcca tatgatgctg
    48961 gatgaaggtg tttacctggc accgtcagcg tttgaagcgg gctttatgtc tgtggcgcac
    49021 agcatggaag atatcaataa caccatcgat gctgcacgtc gggtgtttgc gaagttgtga
    49081 tgattttgcc tcgcccaccg ggcgaggcat tttattaacg gctctgcttt ctcaacaaat
    49141 agataaaata tggcgcgccg ataaaggtcg acagcagccc cgccgggatc tggaacggaa
    49201 acagcaccat ccgcccacac cagtcagcga acaccagcag taaaccaccc accagcgccg
    49261 aaatcacgat gtgcggcatc gtccgtcgaa agcccatcat ccgcgcgata tgcggtgcca
    49321 ttaaaccaac aaaactcaac ggtccaatgg tcatcgtcgc ggtcgccgtc aggcaagccg
    49381 ctaacagcag cagcgcaatt cgcgtcggcg ttaacgccac ccccacggcc cgcgcggtat
    49441 caccacccag cggtaaaatg gtcagccagc ggcggcacag cggggtaatc gccagcaaaa
    49501 tcaccatcac aattccggtg cgccagacct gcgcatcggt ggcgttgtag gtcgaaccag
    49561 agatccaggt cagcacttgc gccattcgcg ggtcaccact cgcctgcaac atcattaaaa
    49621 gcatggtgaa tgcggtactt aacgccatcc ccgccagtaa catgcggtgt ggggaaaatc
    49681 caccgcgccc ggcggcgatc ataataatca acagcgtcac cgccgcaccg atacttcccg
    49741 cgggtaacag ccagccaaaa gcattacccg gtaccagaaa cagcatcagt accacgccaa
    49801 acgccgcgcc agagctaatc cccagcactt ccgggcttgc catcgggttt ccggtcagcc
    49861 gctgaataat acagcccgcc accgccagca tgacgcccgc aaacagcgcc gccataattc
    49921 gcggccagcg ccagggcatc aaatcctcga gcaacgcccc gctcgcccac gtccagccgt
    49981 gcgcatcacg accaaacgac agcgccacca ccacagccat caacagcagc acgccgcccg
    50041 cgagggcaaa cgccagcaca tgttggcgtt cagccgcgac acgatcgttg accttcatat
    50101 ccggcgcgct aatgctgcgt aaacgcggca acagccacag tagcagcggc gcaccgatca
    50161 acgcagtgac cgaaccggtg gacacttcca tccacacgcg agtcagccag aggatgattt
    50221 gatcggaaag ccagaggatc agcgcaccaa tcaacgacgc cagcatcagt cgcggcagca
    50281 gacgccgcgc ccccagcatt tttgccagca acggcgcgaa caacccgata aagccgataa
    50341 tccccaccgc gttcaccagc agcgcactga tgacaatagc cagcgacagc gccgccagac
    50401 gcgccagcga caaggctagc ccgagattgc gcgccacgcc atcatcaagc cccatcagtg
    50461 ttaacggacg aagtagcagc aacgtcagca tcacaccgcc cagcaactgc ggccataaac
    50521 gctcaacacc gccccagtcg gtttgcgtca gcgttccggt gctccacagg aacatgcttt
    50581 gcagttggtc atggtggaag ataaccagta actgattgat tgccccacaa taaaggctca
    50641 ctaccagccc cgcgaggatc agcgttaccg gcgaaagccg tttcccccac gcgacgccaa
    50701 agacgattaa gccaacaacg caagcgcccg ccagcgcagc aaactgactc gccatcgccc
    50761 ctgggatcgc ccagagcgta gtgacggtaa tccccagttg cgcgcctgta gcaacgccaa
    50821 gtgtcgtcgg ttccgccagc ggattacgca gcacttgctg aaacagcacg cccaccagcc
    50881 ccaggcctgc gcccaccagc agcgaaatcg ccagacgcgg cagcaagctg tagtgaaaaa
    50941 tcatctgctc gatgacgtca atatccggcg accaggcagc ctgcgcccac tggctacgcg
    51001 gcagcgcctg cgagaagttc atccaggtca gcgccgtagc ggcaatcacc agcaacgcca
    51061 gcaataatgc cgggaaaagc gcaatacgtt tactcacgct ttacctccaa tggcgttatc
    51121 cagaacgcgc acaaagtgca ttgccgagag agttgcacca taaaaccaga ctgcgggtac
    51181 gcgctgaaag cgtccggcgc ggacaaacgg catggcctgc cacagcggcg ttgccattag
    51241 cgcatccatg tctttgctgt tgtcgtgatc aaaacagagc acatcaacgt ctttatacgc
    51301 tgccagacga tcgatgctga cggcggtact gccccagaag ttggtttccc cttgccaggc
    51361 atttgggatg ccgtactcat caagaatttc ctggaacaag ctgtttggac cgaagaccag
    51421 catatggcgc ggatcgataa gcgtcgtcag caataacgga cgcgcaccac gcttcacaaa
    51481 gcgggggttc atgctgcgga taaagtcttc atattgcgct aaatgcgttt ctgctgcgct
    51541 ttgcaggtta agtaaatccg ccatttccgt cagcgattta cgcgccatcg ccagcggctg
    51601 tttgccgtcg ctgaagttaa atccacgccc cggcgcaata cgagccagca tttctggcga
    51661 aggaccatat cccgccgacc agaccataaa cgacggtttc atttcggtca gcagttcgag
    51721 gttaggttct gtacgcaaac cgacgtcaat caccgattca ggtagtggtg gttcgctgac
    51781 ccacagtcga tagttgatgg tgtctgccac gccataaggc acgatgccga gcgccagtag
    51841 taattccacc ggcagccact ccagcgccac aatacgattt gggtcaatag ccgccgcgtg
    51901 ggcggtattc atctgccata acaacggaga aagcgccatc gccgttaaca gtcgacggcg
    51961 cgaaataaga ggtaagccgc tcatcaataa acaaaactca caggtgcagc acccgccgga
    52021 tgcggcagaa tacccatcgg gatgccataa atcatttcga gggtttcgcc gcgcataatt
    52081 tccgcaggcg ttccctgagc aatcatttca ccgccgcgca gggcgaccag ataatcacag
    52141 taacgcgcag ccatattgat atcgtgcagc acggcaatga ccgtcaggcc acgctcctga
    52201 cttaaacggt gcaccagcgc cagcacatca acctggtggg cgatatccaa cgccgaagtc
    52261 ggttcgtcga gcaacagaca acggctatcc tgcgccacca gcatggcgat ccacgcccgc
    52321 tgacgttcgc cgccagagag gctgtctacc agccgatgcg ccagcggttt taagccaacc
    52381 agcgagatag cttcctcaac tttttcgcga tctgccgcac caaagcgccc tagtgcgcca
    52441 tgccacgggt aacgaccaat cgccaccagt tcacgcacgg tcatcccttc tgccggagga
    52501 agctgctgcg gcaaataagc cactttgcgg gcgaacgctt tgctgctcca gctttccagc
    52561 ggctgggcat caagaagaat ctccccttcc gacggcggct gatgacggcc aagcattttg
    52621 agcagagtgg atttaccaga accgttgtga ccaatcagac cggtcacttt cccggcagga
    52681 aaggttaacg acagcggatg caaaagcgtg cgcccgggca cacgaaagga gatattacgc
    52741 agtgcaaaag tggtatcgga atgattcgtg tattcctgca taacagccaa cttgtgaaac
    52801 aggcacggaa atccgtgccc caaaagagaa attagaaacg gaaggttgcg gttgcaacga
    52861 cctgacgttc tgcgccccag aagcagccat aagtgttaaa gcagctggcg acgtattcac
    52921 gatcgaacag gttgttaaca tgcagcgcca cgttggagcc cgccatgccg actcgcgcca
    52981 gatcataacg cactaacgca tccacgaccg tataacttcc cactttaaag gagttagccg
    53041 gatcaccata gctggagcca gtataacgac caccggtgcc cagcgtcaga cctgaaagcg
    53101 gaccgtcaaa gaaggtatag tcagcccaca gcgaagccat gtgttttggc acctgtgcag
    53161 gcgtattgcc tttataggta gtatcggtgg tgtattccgc atcggtgtaa gtataagaac
    53221 cgactacgtt aacactcgcc gacagcgccg ctttcgcttc gatttctacg ccacgtgcgc
    53281 ggatctcgcc accttcaacc gagaagaagg aaccctcagg gtccgccatc aggttgttgg
    53341 ttttagtgag attatacacg gcaccagtaa ctacaatcgg acgatcttcc ggtacatatt
    53401 tcacgccgac ttcatactgc ttacctttag acggtgcgaa aatattacca tccttcccaa
    53461 cttgcgaaga aggttcaaac gattcgctat agctaaagta aggtgttacg ccattatcaa
    53521 acaggtagtt aacaccacca cgccaggtaa actgtttgtc atcacgttta tcggtcgtcc
    53581 cggcaacgcg gttaagagat tcttgatctg cccagtcata acgaccgccc agggtgacca
    53641 gcactttatc ccactgcgcc tgatcctgaa cataaacgcc cgtttgtttc tgcttattca
    53701 gaatgcggta agggccggag tttgccggat ctttggcatt gaagtcgaaa tcggtattca
    53761 ccggattgta cagatcgagc agcggtacgg agtcgtcgta accaaaccag gcgttgatgt
    53821 cattacgcat acgcataaag tcgacaccgg tcagcagggt gtggtcgata tcgccagtgg
    53881 caaacttgct ctgcaactgg gtatcaacgg agaagttttg cagcttctca tcatcaacga
    53941 cgtatttacg tgccagataa tggcctttat ccgctggcgc taatgccgca cactgtttgc
    54001 tgtaagcatt cgccggatcg gagcagacgc cgtaaccata aacgctgttt tgcgaggttt
    54061 tgttttcagc aaagcgcagg ttctgacgca cagtaaaggt gtcgttaaat tcgtgatcga
    54121 agctgtagcc caccatcttc tcattacgag aataggtgtt gttcttcgcc ccttcgttaa
    54181 agtctgtcgg cagacgctta ccgttcggca gcggctcaac ggttccctct ttcggcaacc
    54241 agccgtaata accggtttcc ggctcgttct ggaagtaaga aaggaaggtg aaattggttt
    54301 tatcatccgg acgccaggtg aacgccggtg caatagcata acgctgctct tctgaccctt
    54361 tctgctgggc attggcagaa cgcgcaagac cggtcaggcg ataagagtaa acgccgtcat
    54421 catccagcgc atcgctaaag tcaaaaccag tctggaacag gctgtcagta ccggctttaa
    54481 actgaacttc tttcagcggt tcggtggtcg gacgcttgct gaccatattc aacaggccgc
    54541 caggactgct tttaccgtaa agcacggaaa ccgggccacg cataatttca gcgcgttcca
    54601 gcatatacgg atcaatgacc gcatcgttat agaagttgcc ctgcaacttc aggccattca
    54661 gatagttatt ctggctttgg ccttctgccg caaaaccgcg aatgatcagg tggtcatagg
    54721 tgttggatgc gccacgcgta ccaacagaga cacccggcgt gtagctaagc gcttctttta
    54781 ccgacttcgg ctgatgcagc gccatctctt cggcggtcac aacagaaata gactgtggca
    54841 ctttttgaat cggcgtatcg gttttagtgc cggtagctga ctgtcgcgcc gcaatagttg
    54901 cagcaggccc ccatgcgctt tcttgcggcg caggtgcagc ggtaacggtg atagtgtctt
    54961 ctttcggttc aaccgctgcc tgtgcataaa cagacatgcc gctaaccgct gtggctacta
    55021 caactgcgat tttacgcagt gagtgttttg gctgagcagt tttggaacgc gccattggta
    55081 tatctctgat gtaaagtgaa tgataacgta aacgagaatg attattataa agataagata
    55141 atattcgaaa ctctggcggg ttcgcaagcg gtacgcaatc tgagcgttcc tgaagggtta
    55201 ataaacaacc agatgaaaag aaagggttaa tactttgggt gggatgttat tttaccggat
    55261 ggcaactcgc catccggtat ttcacgctta gatgttaatt actaccaaac atatccttaa
    55321 tccaaccggc tacaccgtcg ctgtctttct gctcttgctg agcaggttgc tgttgcggct
    55381 gttgctgcgg ctgagaagac tgatcaaacg gattgcctga cggctgctgc tgcatctcac
    55441 tctgctggca cagcgattgc ggatcgctgg tccagaccgg caagacacgc atgccaccgc
    55501 tgcaaacaaa gttgccgtcg tagtccacgc ccatatctgc aatatcttct ggcggaacaa
    55561 gattcagcgg cgttggcgtc tggttagcca gataacgctg ataaatcgac attgccccgc
    55621 tggctccata cagtttggtc ggctggttgt tatcacggcc gacccaggtg atggtcaccg
    55681 tgctgccgtc aatgcccgca aaccaggtat ctacgttatt gttggtagtc cctgttttcc
    55741 ctgccagatg caggttcggg tatttcgccc caagctgacg acccgtaccg cgttgtacca
    55801 cctgctgcat ggtccatagt gtcagatacg ccgcctgcgc cggaacagcg cgttccgcct
    55861 gcgggaagct ctgatacagc actttgccat cttccgcgat taccgaacgc agcgcagaaa
    55921 gcggtgcacg gttaccaccg ctggcgatgg tctggaatgc ctgcgccact tcgattggcg
    55981 ttaagttcaa cgcccccagc agcattgccg gaaccggatg caactgatct ttcggtacgc
    56041 ccagtttaat ccaggtatcc gtaaccgcag gcagccccag cgccatcccc agatttaccg
    56101 tcggcacgtt catcgaacgg gtcaacgcat ccaccagcat cactctgccg ctttcgctat
    56161 aacgacggtc atcattctgc ggtgaccaga cctggccatt cggctgacgc agcgcaattg
    56221 gcgcatccgc aatccacgta ttcagacgat agattttcgg ctggcttaag gccgtcagat
    56281 aagtcgctgg ttttgcaagg gaaccaatcg aacgacgcgc ctgcatcgca cggttgtagc
    56341 ccgcaaactg cggctcagaa cctccgacca tcgcacgaac ttcaccacta aagcggtcga
    56401 cgaccacaat cgcagtttca agatcgctca acttacgctg tttcttcagt gccggaatac
    56461 cttccacggc ggctttttct gccgcgtcct gggccaccga gtcaaaggta gtgaagatct
    56521 tcacgccgga gagatctttt accttatcgc ctaatttagc ctgaagctcc tgacgcacca
    56581 gttgcataaa ggctggctga ggagagatca ccccaccgcg cggctgaacc cccagcggac
    56641 gcgcactcaa catgtcatag agttcttgat caataatctg ttgctgttgc agcagacgca
    56701 gtaccagatt acgtcgctcc agcgccagtt tcgggttacg ccacgggttg tagatggacg
    56761 ctcctttcac cataccgact aacagcgcct gctgatcgag gctcaattct tctaccgggc
    56821 gaccaaagta atacaagctc gccagcggga agccgcggat ttcgttgtcg ccgctctgac
    56881 cgagatacac ctcgttcata tacagctcaa gaatacggtc tttgctgtaa cgcgcgtcca
    56941 tgatcagcgc catgtaggct tcattcgctt tacgccaata agagcgctcg ctggagagga
    57001 acaggttttt caccagctgt tgcgtcagcg tactcgcccc ctgcaccgtg cgacctgcgg
    57061 tcaggtttgc cagcaccgca cgtccgatgg agtagagact gattccatca tgctcgtaga
    57121 aatggcggtc ttctgtcgcc agcaaagtat ccaccagcaa atccgggaaa ccactgcgcg
    57181 gcacaaacaa acgctgctca ccgtttggtg aggagatcat ggtgatcaga cgcggatcaa
    57241 gacggaagaa accgaactga cggttgttct ccatattgac gatggtcgcc agatgatcgc
    57301 catcaaaggt cagacgcgcg cgcacctgcc cttctttact gtccgggaaa tcaaacggac
    57361 ggcgaatcat ctcaatgctg ttggcctgca cggtaaattc gccaggacgg gtcattttcg
    57421 acacctgacg atactgtgtt gcctccagca gcttcaccat ctcgttcttg ctgatggtca
    57481 tgtctggctc aaggttgacc attcgaccat aaactgccgc aggcagttgc cagaccttgc
    57541 catcaatacg gctacgaatt ttttgatcga gataaacgcc gtaaatggcg atcagcacgg
    57601 caaaaacgat agccagtttt aacagcagcc atagccagcc gcgtttgcca cgaggcttac
    57661 gccctttgcc tttgccctta cctttgcgcg gcatcggttc ttcatcctca tagtcatcat
    57721 aatcgtcgta atcatcgtca tcttcgtaac gacgacggct taccttttgt ttgaccggac
    57781 gcgtcggttt ccctttgcgt ccaattggct cgcggtcatt cccggccatg ctttttctcc
    57841 gcaatattca ggcgcaaaag cccgattttc tgttcttctg ttacaagaca gaagaagata
    57901 tctctcaaaa tttgagctcg atcggtcccc tctcccttaa gggagagggt tagggtgagg
    57961 gtgaacgtgc gcgctggtgc cctcaccccg gccctctccc acagggagag ggagaaaacc
    58021 aaccttacga atactttttc gtccgtcgcg tcggtgccgt gtttgccggg tcgtccggcc
    58081 agacgtgttt gggataacgc cctttcatct ccttttgcac ctcacgatac gcccctttcc
    58141 agaaggcccc caaatcacgc gtgatttgca gcggcctttg ggcgggtgaa agcaattcca
    58201 gcaccagcgg cacgcgcccc tgggcgatcg tcggattagt ggcctcacca aacatctcct
    58261 gcattctcac cgccagcgcg ggcgggttat cttcatgata acgaatggcg atccggcttc
    58321 ccgtcggcac agtgtaatgc gcaggcaatt cactatccag acgttgctgc attccccaat
    58381 caagtaatcc gcgtagtgcc tgataaatgt cgagtgattt caggccgcgt agtgaatgta
    58441 caccggccat atgtggcagc agccacgttt ccagcgtcgc caacaaactt tcatcatcaa
    58501 ccgctggcca gtcatattct ggcaaccact ttccggcgca taacaaacgc aagcgtagct
    58561 gttccgcttc cgccgtccag ttgagcacgc ttaaaccttt atcacggatg ccgttaagca
    58621 tcgcctgatg caactcgtct tctgacggtt tcgccaacgg ctgcactttc accgtcaact
    58681 gaccgatttg cagccgacgc caggctttca gcgtaccttg cgcgtcatcc cattccacgg
    58741 tgtcagattg ctgcaccagc tgcggacagc gttgtactaa ttcatcaata tcgaccggta
    58801 acgccagtaa aatccgcgca tccggcgagg cgctgccctg caataataac ggcgcgatca
    58861 accattcgtg gcgacttagc gcgtcgtcgg catcaagcat cgcccccatg ccgtttgcca
    58921 gttgatagcg tccatcttgc ccacggcggc gagcaatgcg atcggcaaac gccccggcaa
    58981 gtagcggcgc gataagcgaa ctgtctgcct caccgccgcg tacgtttaag cgttttaaca
    59041 gttgctgact acgttgctgc caggctggtt gattgcgcga aaacgccacg cccaggtcac
    59101 tattgcccat ccgtggcggc tcttcgagaa tggcggcaat ttttgccgcg gtagcagctt
    59161 cgtcgtcgct ctttgcgcta accagcattg ccgctaaacg cggatcgtta cccagcgctg
    59221 ccattttttg cccttgcgca ctaagccgtt caccgtccag cgcccccagc atttgtaaca
    59281 gacgtttcgc ggccagtaga ttcaccgttg gcggttgatc cagccagctc atctgcgccg
    59341 gatcgctgca tccccattgc agtaattcca tcagcaaacc ggaaagatcg ctttgtaaga
    59401 tctccggttc actttgcgcg gtagcgcgtt ctgcttgttc tttggcgatt aaatgcaggc
    59461 agatacccgg ctccagacgc ccggcgcgcc cggcacgctg cgtcatcgac gcctgactga
    59521 ttcgctgagt aatcaaccgc gtaagccctg ttcgcggatc aaaacgcgcc accctttcct
    59581 gggcacaatc caccaccaga cgaatacctt caatggttaa actggtttca gcaatattgg
    59641 tcgccagcac cactttgcgc atcccttgcg gtgccgggag gatcgctttt cgctgatcgt
    59701 tcagcgacaa cgcgccatac agcgggcaga gcaatacatc actgccgatg cgcgaagcca
    59761 gttgttcctg cacacgctga atttctccga cgccaggtaa aaataacagt aatgatccgc
    59821 tttcctgacg cagcatttca gcggtggcta ccgcaacggc atcgtcaaaa cgctgatgcg
    59881 tgggcagcgg taaataacgg cgttcaaccg gaaacgagcg cccttctgag atgacgacgg
    59941 gcgcttctgg cagcatttgc tgcaagcggt cgttgtccag cgtagccgac ataatcagca
    60001 gtttaaggtc atcacgcaga ccttgttgca catcgagtaa cagcgccaac gccaaatccg
    60061 cctgcaagct acgctcatga aattcatcaa ggatcaccaa tccaacaccg ctcagttccg
    60121 ggtcacgctg gatcatccgc gtcagcacgc cttcggtaac tacttccagg cgggtattcg
    60181 gcccgacgca gttttgcgca cgcatccggt agccaacggt atcgcctggc ttttcgttaa
    60241 gcagctccgc cagccgttgc gcgacgttac gcgccgccag acgccgtggc tccaggagga
    60301 taattttccc gttaatgccg ggatgcgcca gcagttgcag cggcagccag gttgatttcc
    60361 cggccccggt cggcgcactt aataatacct gcggcgcata atcgagagcg gtaagtaatt
    60421 caggtaacac ggcagcaacg ggcaacgacg acacaaaacg ctccagaggg ttaacattct
    60481 tcgcgccaca ttgtagcatc gcggtaattc ataaccgagt gcctcacatg tctgacccgc
    60541 aacgtctgtt ctttgctatc gacttacctg cagaaatccg cgaacagatt atccactggc
    60601 gcgccacaca cttcccactt gaggcgggac gtccggtcgc cgccgataat ttgcatctga
    60661 ctctggcatt tttaggcgaa gtgagcgcag agaaagagaa ggcgctttct cttttagccg
    60721 gacggattcg tcaacctggt ttcacactca cgcttgatga cgccggccaa tggctgcgtt
    60781 cgcgtgtggt gtggttaggg atgcgtcagc cgccacgcgg cttaatccag ctggcgaata
    60841 tgctccgttc acaggctgcc cgcagcggtt gttttcaaag caatcgtccg tttcatccac
    60901 atattacctt attgcgcgac gccagcgagg cggtgacaat cccgccgcca ggttttaact
    60961 ggtcatatgc ggtaacggag ttcacccttt acgcctcctc gtttgcccgt ggacgcacac
    61021 gctacacgcc gctaaaacgc tgggtgctaa cgcaataaca aggattgtcg caatggaatt
    61081 ttctccccct ctacagcgcg cgacgctaat tcagcgttac aaacgttttt tagccgatgt
    61141 gatcacaccc gatggtcgcg aattaacgct acactgcccg aatacgggtg cgatgaccgg
    61201 ttgtgcaacg cctggcgata ccgtctggta ttcgacttca gacaacacca aacggaaata
    61261 cccacacacc tgggaattaa ctcaaagcca gagcggcgca tttatttgcg tcaacacgct
    61321 ttgggctaac aggttgacga aagaggctat ccttaatgaa tcaatttcag aactgtcagg
    61381 ctatagctcg ctgaaaagcg aagtaaaata cggcgcagaa cgcagccgta ttgactttat
    61441 gttgcaggcg gattcgcgtc cagactgcta tattgaagtg aaatcggtta cgttagcgga
    61501 gaacgaacag ggatattttc ccgatgcggt cactgaacga ggtcagaaac accttcggga
    61561 gttgatgagc gtagcggctg aaggccagcg tgcggttatc tttttcgccg tgctgcattc
    61621 agccattaca cggttttcac ccgcgcgcca catcgatgag aaatacgcgc aactattgtc
    61681 agaagctcaa cagagggggg tagaaattct ggcttacaaa gcggaaattt ctgctgaagg
    61741 catggctctt aaaaaatcac tgccggttac attgtagtaa agtaagtaac tggttaattt
    61801 acattctggt cgcgtgcgca aatacgcttt tcctcacaca gttgtcaagt gttacgttta
    61861 gataattgct atccggaaaa gcatctgcta tttatagcgg cctcattttt cccccgaaca
    61921 tggggatcga tagtgcgtgt taaggagaag caacatgcaa gaagggcaaa accgtaaaac
    61981 atcgtccctg agtattctcg ccatcgctgg ggtggaacca tatcaggaga agccgggcga
    62041 agagtatatg aatgaagccc agctggcgca cttccgtcgt attctggaag catggcgtaa
    62101 tcaactcagg gatgaagtcg atcgcaccgt tacacatatg caggatgaag cagccaattt
    62161 cccggacccg gtagaccgtg cagcccagga agaagagttc agcctcgaac tgcgtaaccg
    62221 cgatcgcgag cgtaagctga tcaaaaagat cgagaagacg ctgaaaaaag tggaagacga
    62281 agatttcggc tactgtgaat cctgcggtgt tgaaattggt attcgccgtc tggaagcgcg
    62341 cccgacagcc gatctgtgca tcgactgcaa aacgctggct gaaattcgcg aaaaacagat
    62401 ggctggctaa ttacagccgt tccatcacgt ttaccacagg cgggaaatgc tcccgcctta
    62461 ttttttgttc aaagagatga cagacacaca gtatattggc cgcttcgccc cctctccttc
    62521 cggcgagctt cattttggtt ctctgattgc tgcgcttggc agctatttgc aggctcgcgc
    62581 ccggcacggt cgctggctgg tacgcattga agatatcgac ccgcctcgtg aagttcccgg
    62641 tgccgcagaa actatcctgc gccagctgga acattacggt ctgcactggg acggcgatgt
    62701 tctctggcaa tcgcaacgtc accacgccta tcgtgaagca ctcgcctggt tacatgaaca
    62761 aggactaagt tattactgca cctgcacgcg tgcgcgtatt caaagcattg gcggtattta
    62821 cgacggtcat tgccgggtgt tgcatcatgg accagacaac gccgcagtgc gtatccgcca
    62881 gcagcatccg gtcacgcaat ttactgacca gctgcgcggc attattcacg ccgacgaaaa
    62941 actggcacgg gaagatttta ttattcatcg ccgtgatggt ttgtttgcct ataacctggc
    63001 ggtggtggtg gatgatcact tccagggcgt gagcgaaatt gtccgcgggg cagatttgat
    63061 cgaaccgaca gtgcggcaaa tctcgctgta ccagcttttt ggctggaaag tgccagatta
    63121 cattcatctg ccgctggcgc ttaatccaca aggcgctaaa ctttccaagc agaatcatgc
    63181 gcctgcgttg ccgaaaggcg atccacgccc ggtactaatc gcggcacttc aatttctggg
    63241 gcagcaggca gaagcacact ggcaggattt cagcgtcgag caaatccttc agtcagccgt
    63301 caaaaactgg cggcttaccg cagtgcctga gtcggcaatt gtaaattcaa cattctcaaa
    63361 tgcgtcatgc tgagctatga ttagccgcta tttttttgtc ctgaatgatg tttgacacta
    63421 ccgaggtgta ctatttttac ccgagtcgct aatttttgcc gcaaggtgct aagccgcgag
    63481 gaaagcgagg ctgaacaggc agtcgcccgt ccacaggtga cggtgatccc gcgtgagcag
    63541 catgctattt cccgcaaaga tatcagtgaa aatgccctga aggtaatgta caggctcaat
    63601 aaagcgggat acgaagcctg gctggttggc ggcggcgtgc gcgacctgtt actcggcaaa
    63661 aagccgaaag attttgacgt aaccactaac gccacgcctg agcaggtgcg caaactgttc
    63721 cgtaactgcc gcctggtggg tcgccgtttc cgtctggctc atgtgatgtt tggcccggag
    63781 attatcgaag ttgcgacctt ccgtggacac cacgaaggta acgtcagcga ccgcacgacc
    63841 tcccaacgcg ggcaaaacgg catgttgctg cgcgacaaca ttttcggctc catcgaagaa
    63901 gacgcccagc gccgcgattt cactatcaac agcctgtatt acagcgtagc ggattttacc
    63961 gtccgtgatt acgttggcgg tatgaaggat ctgaaggacg gtgttatccg tctgattggt
    64021 aacccggaaa cgcgctaccg tgaagatccg gtacgtatgc tgcgcgcagt acgttttgcc
    64081 gccaaattgg gtatgcgcat cagcccggaa accgcagaac cgatccctcg cctcgctacc
    64141 ctgctgaacg atatcccacc ggcacgcctg tttgaagaat cgcttaaact gctacaagcg
    64201 ggctacggtt acgaaaccta taagctgttg tgtgaatatc atctgttcca gccgctgttc
    64261 ccgaccatta cccgctactt cacggaaaat ggcgacagcc cgatggagcg gatcattgaa
    64321 caggtgctga agaataccga tacgcgtatc cataacgata tgcgcgtgaa cccggcgttc
    64381 ctgtttgccg ccatgttctg gtacccactg ctggagacgg cacagaagat cgcccaggaa
    64441 agcggcctga cctatcacga cgctttcgcg ctggcgatga acgacgtgct ggacgaagcc
    64501 tgccgttcac tggcaatccc gaaacgtctg acgacgttaa cccgcgatat ctggcagttg
    64561 cagttgcgta tgtcccgacg tcagggtaaa cgcgcatgga aattgctgga gcatcctaag
    64621 ttccgtgcgg cttatgacct gttggccttg cgagctgaag ttgagcgtaa cgctgaactg
    64681 cagcgtctgg tgaaatggtg gggtgagttc caggtttccg cgccaccaga ccaaaaaggg
    64741 atgctcaacg agctggatga agaaccgtca ccgcgtcgtc gtactcgtcg tccacgcaaa
    64801 cgcgcaccac gtcgtgaggg taccgcatga cagtggcgta tattgccata ggcagcaatc
    64861 tggcctctcc gctggagcag gtcaatgctg ccctgaaagc attaggcgat atccctgaaa
    64921 gccacattct taccgtttct tcgttttacc gcaccccacc gctggggccg caagatcaac
    64981 ccgattactt aaacgcagcc gtggcgctgg aaacaacact tgcgcctgaa gaattactta
    65041 atcacacaca gcaaatagag ttgcagcaag gtcgcgttcg taaagcagaa cgctggggac
    65101 cgcgcacact cgatcttgat atcatgttgt ttggtaatga aaccattaat accgaacgac
    65161 tcaccgtccc gcattacgat atgaagaatc gcggctttat gctgtggccg ctgtttgaga
    65221 ttgccccgga actcgtgttt cctgatgggg agacattacg tgaaatactc acttcgctag
    65281 cagtactaca acctgccatc tggtattagc tgtattattt tattttctta tcttactcct
    65341 cacttcccct cttacagatt aaatatactt aatttacatt ttagtttttt ttaattaatt
    65401 catgaaatag cacttcatgc aaatagatta ggcatttttt gtttaaaaaa cctagaatag
    65461 caacaattca atacaccacg taaatatatt cacacccgtg ataattcacc cctattacat
    65521 cctccaaaaa ctttgagtaa tatccgctcg caatattaat ttcatataat ttttgtaagg
    65581 atgcatgtaa tgaaaaaagc acttctcgca gccgctctgg ttatggcttc tggttccgcc
    65641 ctggctgtag atggtggtca tatcgacttt aacggtatgg tacagtccgg tacctgtaaa
    65701 gtgggtgtgg tagatactgg tatgcatagc gttaccactg atggcgtggt taccctggat
    65761 actgcgaatg ttactgatac ttttgctgaa gttagcgcaa ctgctgtcgg tttactgccg
    65821 aaagagttca tgatttctgt tgagtgtgat ccaggtgctc cgaagaatgc tgagttaact
    65881 atgggttctg caagttacgc gaacaccagc ggtaccctga ataacaatat gaacatcact
    65941 gttaacggta ttgcaccggc tcagaacgta aacattgcag ttcataacat gaaaaacaaa
    66001 gctggcgctg ctgaaattaa gcaggtccat atgaacaact cttctgaagt tcaggaactg
    66061 acattagacg cagaaggtaa aggccagtac gtatttaacg catcttacgt taaagcaccg
    66121 aacagcccgg ctgtaactgc tggtcatgta accactaacg cgctgtacac cgttgcttat
    66181 aagtaattga tggtgtgttg agcataaggt ttgccagcag gcaaacctta ttttcgcgaa
    66241 tttaatcttt ctacagacag caaggagtgc ttatgtttcc cttggttaaa aaaacggtta
    66301 gtgcattatt tgtgagtact ctgttagcca gtgcaccagc attcgctgac attattattt
    66361 ccggaacacg tatcatttat aacgctgata aaaaagatgt taacgttcgt ctggaaaata
    66421 aagggaatcg ccccttactg attcagaact ggttggatac gggcgatgac aatgccgatc
    66481 ccagccagat caaagtgccg tttaccgcga ccccaccagt ttcacgcgtg gagccaaaac
    66541 gcggtcaaac cgtaaaagtg atgtataccg gtgcaacagc cctgccgaaa gaccgtgaaa
    66601 gcgtctactg gtttaacgtg ctggaagtgc cgcctaagcc gaaagatgct gaggctgaca
    66661 aaaacttgtt acagctggct ttccgcacac gcatcaaact cttttatcgc ccgagtgggt
    66721 tacagggaga accggctgaa gcaccggcga aaatcacctg gaaattaaac aacgctcagt
    66781 tacaggcaaa taacctaacg ccatattacg tgtcctttaa tgaggtcaag ctggaaagcg
    66841 gcggcaaaac ctacaacgtc aattccagca tggtcacgcc gttcagccag gcaagctttg
    66901 gcgtaaccag tctgccagga agcgtgtcat ccggaaaagt ggtctttaaa gcgatcaacg
    66961 actttggcgg caacattgat ggttccgcaa cattctaaaa aataaacgga ttctttttaa
    67021 catcgccagg aatgcaacct tgtaccagtt tactcatcaa aaaagccgta tcccgaaaaa
    67081 aacgctactt gcggcctgtt gtgccctgtt ttatagcagc aacggtgctg cggcggacac
    67141 cgtggaatat gacagttcct ttttaatggg aactggcgca tcaacgattg atgttaaacg
    67201 ttatgctcaa ggcaacccga caccgccggg tctctataat gtccgcgtat ttgtaaacgg
    67261 tcaggcgact tccagcttag aaattccgtt tgtggatatt ggcgaaaaca gtgcggcggc
    67321 ctgtcttacc cataaaaacc tggcgcaact tcacattaag caacctgaac agcctgtcac
    67381 tttactcgcc agagaaggtg aagaagagga ttgtctggat ctggcaaagt catacgaaaa
    67441 ggcggatgtg tgctttgacg gtagtgacca gtttctcgat ctgacgatcc ctcaggccta
    67501 tgttctgaaa agctatggcg gctacgttga cccttcttta tgggaatcgg gaattaacgc
    67561 tgccacactg gcatataccc tgaacgcgta tcacacaagt tcagataacg acaatagtga
    67621 cagcgtctat ggcgcgttca actcaggtat caatttagga gcctggcact ttcgtgcgcg
    67681 cggtaactat aactggacaa cagataacgg cagcgatttc gatttccagg atcgttactt
    67741 acagcgtgac attccggcaa tccgttccca gataattatg ggtgatgcct ataccaccgg
    67801 tgaaacgttt gactctgtca acgtccgtgg tgttcgcctg tacagcgaca gccgtatgct
    67861 gccttcggcg ctggccagtt acgctccgac catccgcggt gtagcaaact ccaacgccaa
    67921 agtcaccgtg acgcaaagcg gatataaaat ttatgaaacc accgttccgc ccggtgaatt
    67981 tgttatagac gacattagcc cttccggctt tggtagcgaa ctgatcgtga ccattgaaga
    68041 agcggatggt tccaaacgca cctttacgca acccttctcg tcggttgtac aaatgcaacg
    68101 tcctggtgtg ggccgttggg atttcagcgc gggtaaagtc attgatgaca gtctgcgatc
    68161 cgaacccaat atggggcaag cctcttatta ctatggtctg aataacctct tcacgggtta
    68221 taccggcatt cagttcaccg ataataacta tcttgccggg ctgttaggtg tgggtatcaa
    68281 caccagcatc ggcgcctttg cggtagacgt tacccattcc cgtgctgaaa ttccggatga
    68341 taaaacctac caggggcaaa gttatcgcgt gacctggaac aaacttttcc aggataccgg
    68401 gacatcattt aacctcgcgg cgtaccgcta ttccacccag gattacctgg gcctgcatga
    68461 tgcgttagtc ctcattgacg acgccaagca tttgtctgcc gatgaagaca aaaacaccat
    68521 gcagacgtac tcacgtatga aaaaccagtt taccgtcagc attaaccagc cattgaatat
    68581 cgcctatgaa gattacggtt cgctgtttat ttccggtagc tggacgtatt actgggcggc
    68641 gaacaatagc cgcactgaat ataatgttgg ttacagtaaa agcgtttcgt ggggcagttt
    68701 cagcgtcaac ctacaacgta gctggaatga agacggcgag aaagatgacg cgatgtacgt
    68761 cagcgttagc gtacctattg agaatatttt aggtggcaaa cgtaagtctt ctggtttccg
    68821 caatttaaat actcagctca ataccgattt cgatggttca catcagttga atgttaacag
    68881 ttccggtaac actgaaaaca atctggtgaa ctacagtgtc aacgcaggtt atagcctcga
    68941 taaaaacgcc ggcgatttag cctctgttgg tggttatctc aactatgaat ctgggttagg
    69001 cggtatttcc gcttcggcct cggccacttc tgataacagc caacagtact ccatctcaac
    69061 cgatggcggc tttgtattac acagtggtgg tttaacgttc actaacaaca gtttcagcag
    69121 taacgacacg ctggtgttaa tcaacgccct aggtgctaaa ggcgcacgaa tcaataacag
    69181 taataacgaa atcgatcgct ggggatatgc cgtgacgtcc tctgtcagcc catatcgtga
    69241 aaaccgggta ggtctgaaca ttgaaacact ggaaaacgat gttgaactga aaagtaccag
    69301 cgccaccacc gtaccacgta gcggctccgt tgttttgacc cgtttcgaaa ctgacgaggg
    69361 gcgttctgcc gtgctgaata ttactgccgc caatggcaaa tccattccgt ttgctgcgga
    69421 ggtttaccag ggtgaggtga tgatcggcag catgggccag ggtggtcagg catttgtacg
    69481 cggtattaac gacagcgggg aattaatcgt gcgctggtat gaaaacaacc aaaccattga
    69541 ctgtaagttg cactaccagt tcccggcgca gccacaaacg cagggaagca ccaacacctt
    69601 attacttaac aatcttacct gtcaggtagc aaatcactaa tatggaaaat tctatgaaaa
    69661 ggatactact aacatccgcg ttaataggcc tgggtttacc ggcggttggc tctgcaacag
    69721 atcttaatgt cgattttacg gccaccgtat tagccaccac ctgcacgata actatagtgg
    69781 aagatggtgg tccagcagtg accggtagta acgatgaata cagcctgaca attcctgatg
    69841 ttggtctgga taaagtcgct accgctgcgc ccgaagctca ggccaacttt aaattaaagg
    69901 cctccgattg cagcaatggt tactcaaaga tttttaccac acttacagga acaacggttt
    69961 ccggtaagtt aattgttaat gaagcaacct ctggcgctgc tggggtgggg atggggatta
    70021 agcgtagaga taccgcagac tcgactttct tcacacccaa taatactgac aaatttgaat
    70081 ggagcgccga tgagaaagcc agcggtgtgc ctctgaccgt agcactgcgc gaaacaacgg
    70141 cgggagcagg acgcaccggt gcgtttcagg caaaagccac ctttaacttc acctacgaat
    70201 aacagggaca gccaccatga aattaaaagt catcgctaca ctgattgcta ctgttgccgt
    70261 gggtgtaagc tttaacagca attttgcttc tgcgagtaca acgtccgctt ctttaaccgt
    70321 aaacagtaac ctgactatgg gtacctgcag tgctcagata atggataata gtaataaagt
    70381 gatcaatgaa gtggtctttg gcaatgttta tatttctgaa ctcggtgcaa aaagcaaagt
    70441 gcaacagttt aaaattcgct ttagcaattg ctctggcctt ccccaaaaca gcgcccaaat
    70501 agtgctggca cctaatggta tatcctgtgc tggttctcaa tcgtcatcgg cgggtttttc
    70561 taacaagttt actgacgcta gcgcagcaac cagaacggct gtggaagtat ggactacaga
    70621 tacaccggaa agcaatggca gtacgcaatt ccattgtgct caaaagatac cagtgcctgt
    70681 gacgcttccc gccgacacca caactcagcc ttacgattac ccgttaagtg cacggatgac
    70741 cgttgcggaa ggtagattgg taaccgatgt aagaccgggt aatttccgct ctcccacgac
    70801 tttcacgatc acttatcagt aatagcggat aaggatatta gaatgctatg caggcatcat
    70861 aaaatcgtac actttttagg gctggcaaca gcccttatca cgccttttgc ctattccggc
    70921 caggatgtgg atctcaccgc aaagatcgtg cccagcacct gtcaggttga agtcagtaat
    70981 aatggcgttg tcgatctcgg cacagtgacg ctggactatt ttgccgacaa tgtaaccccg
    71041 acaacggatt atgcgggtgg caaaacgttt aatgtgaatg tggtctcttg cgataacatt
    71101 caaacgacac agtcgcaaat gaaattagat tttcagccgc aggcgggatc gcttgcacag
    71161 gcgaataatc aaatttttag taatgaatat gaacagcagg ctaccggggc gaaaaacgtc
    71221 ggcattgtga ttttttcggc ccaaccgaat cagcagacat ttaacgtgcg aggaacggat
    71281 ggttcctcaa cagcaattta ttcggtagca cctggcaatg ctgttccctc aacctggacc
    71341 ttttattccc gtatgcagcg ggtgaataat gcccttcctc ctgaatcggg aatggtgaga
    71401 agccaggtta ttgttaacgt atcttacgaa taataaggat aattatgcaa aggaaaggca
    71461 ataaactgtt gattcagtta tgcagtgtga tactgctatt ttttaccaca tcctggtatg
    71521 cattggcgaa tgaatgttat atagagagaa atgctgaagg ggattatcac atgaagataa
    71581 gctctactca gcttagtctg gcgtcacaaa tggtcgaggt tccgacagaa atagccgaag
    71641 ctacatggga tgtaaatatt caactaagag gcgatgccat agggtgtaaa tctcttgggg
    71701 atagtaaggc agttcacttt cttaatacag ctgacccaag tttaatatcc acgtacacca
    71761 caacgaatgg cgcagcgtta ttaaaaacaa ctgttccagg cattgtgtat tctgtcgagt
    71821 tattatgcct tagttgtggt gccgcagatg aacttgattt atggctacct gcacaaagtg
    71881 gcgcagataa cttcatacca agcacccaga cgaaatgggc ctatgagtac agtgatcaaa
    71941 gttggtattt acgttttcgc ttattcataa ctcctgaatt taaacccaag aatggtgttt
    72001 ccagcggaac aacgatagca ggaaagattg cgtcatggta tataggtacc aatgaccagc
    72061 cgtggatcaa cttttacatt gacaatgact ctttaaagtt tttcgtcgat gaaccgacct
    72121 gtgcaacagt tgccctggca caagatcagg gcaacgtcag tggcaatcag gtaacgcttg
    72181 ggaacagcta tgtttcggaa gtgaaaaatg ggcttacgcg ggaaatccct ttttctatcc
    72241 gtgctgaata ctgttatgcc agtaaaatta cggttaagtt gaaagcggca aataaaccca
    72301 gcgatgccac actggtgggt aaaacgactg gctcggtttc aggcgtggct gtaaaagtaa
    72361 attcaactta tgacaatagc aaagtattgt taaaagcaga tggtagcaac acggttgact
    72421 acaacttcgc cgcctggtca aacaacctgc tgtttttacc ttttacggcg cagctggtac
    72481 cggatggtag cggtaatgct gtcggtgttg gaacattttc aggtaacgcg accttctcct
    72541 ttacctacga ataagtaaaa cgtcattccc tcctcatcgc accagagttc tgctctggtg
    72601 cgaacatttc cccacactaa taaccattgc ctccctgttt tcggctgtta gaatgcgatc
    72661 aatctgactt tagccatcag gatacgttat gaaaccgacc accattgctt cattgcagaa
    72721 atgtaagcag gaaaaaaaac gcttcgccac catcaccgcc tacgactaca gcttcgcaaa
    72781 actgtttgcc gaggaagggc tgaacgtgat gctggtgggc gactcgctgg ggatgaccgt
    72841 tcaggggcat gaatctaccc ttcccgttac cgttgaagat atcgcctacc acactaccgc
    72901 agtacgtcgt ggcgcgccaa actgcctgct gttggctgac ctgccgttta tggcgtatgc
    72961 cacgccggaa caagcctttg aaaacgccgc aacggttatg cgtgccggtg ctaacatggt
    73021 caaaattgaa ggcggtgagt ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt
    73081 tcctgtatgt ggtcacttag gtttaacccc acagtcagtg aatattttcg gtggctacaa
    73141 agttcagggg cgcggcgatg aagcgagcga tcgactgctt agcgatgcat tagccttaga
    73201 agccgctggt gcacagttgc tggtgctgga atgcgtgccg gttgaactgg caaaacgtat
    73261 taccgaagcg ctggcgatcc cggttatcgg tattggcgca ggcaacgtta ccgatgggca
    73321 gatcctcgtg atgcacgatg cgttcggcat taccggcggt catatcccta aatttgccaa
    73381 aaatttcctc gctgaaacgg gcgacatccg cgctgcggtg cggcagtata tggctgaagt
    73441 ggagtccggc gtttatccgg gcgaagaaca cagtttccat taaggagtga cgttgtgtta
    73501 attatcgaaa ccctgccact gctgcgccag caaattcgcc gcctgcgtat ggaaggcaag
    73561 cgcgtggcgc tggtacctac catgggcaac ctgcatgatg gccacatgaa gctggtcgac
    73621 gaagccaaag cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc
    73681 gaccgcccgg aggacctggc tcggtaccca cgcaccttgc aggaagactg cgaaaagctg
    73741 aacaagcgca aagtggatct ggttttcgcg ccctcggtaa aagagatcta cccgaacggt
    73801 actgaaaccc atacttacgt tgacgttcca gggctttcga ccatgctgga aggcgccagc
    73861 cggccggggc attttcgcgg cgtgtcgact atcgtcagca agctgtttaa cctggtccag
    73921 ccggacatcg cctgcttcgg tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg
    73981 gttgctgata tgggctttga tattgagatt gtcggtgtgc caattatgcg cgccaaagac
    74041 ggtctggcac tgagttcccg taacggttat ctgacggcag aacaacgcaa aattgcgccc
    74101 ggtctgtaca aagttttaag ttcgattgcc gacaaattgc aggctggcga acgggatctc
    74161 gatgaaatta ttgctattgc cgggcaagaa ctgaatgaaa aaggcttccg cgccgatgat
    74221 attcagattc gcgatgccga cacattgctg gaagtctctg aaaccagcaa acgggcagta
    74281 attctggtag ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg
    74341 gcgtaatact tatactctaa ataattcaag ttgcaagttg caggaaggcg gcaagcgagt
    74401 gacaaattcg tcaggaacga atttgcccag ccgcaggctg gccttcggtg agagacatgg
    74461 atgtctctca ttaatcccca ggagcttaca taagtaagtg actggggtta gcgaacgcag
    74521 acgcagcaca tgcaatttga agtatgacga gtataactgg cgctacggct gatggcgcca
    74581 attattaatt tgccccacgt catggcgatg gcgtctgtaa gcaattattc ataacgcaga
    74641 caacttagtt aatcagccta tcaatctcag caagagataa atttgtcatc tctgcaacgt
    74701 cttcccgaga catcccttta ctcagaaaac gctgggcgaa ttcttgcctc tcctcctgtc
    74761 ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgtgcc agcgtcatca
    74821 tagacttccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag
    74881 tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgactttcg ctagtgtacc
    74941 cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat
    75001 gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg
    75061 tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgcgcc agctccggcg
    75121 agtaaaacat atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa
    75181 agagaatcgg cactaccagc ggtagtttgt cgtgatccgc ctccagatgc cgatgcatgg
    75241 cggctataga ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttggtgtt
    75301 caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag agcacgtccg
    75361 tgctgtgtcc tttaaggctc tcttcaatga aactccccga ctctaaatga agcgtgttga
    75421 ggtcacaaag ttcgcgtaat tccactggca aatgtatctc cagaaagtcg cgagccgtct
    75481 ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca
    75541 tcttccctgt cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgtgctatcg
    75601 tcagccacct ctttattgtt taccgagcag cgttcagaga aatatcaatt cgttacaggc
    75661 gatacatggc acgcttcggc gcggtacgca aactgctgat gcaaatgccg tcagatgagt
    75721 agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcaccgcct
    75781 gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa
    75841 actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga
    75901 ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccattgata tctggaatgt
    75961 caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat
    76021 ttctgttaac ggtgcggcgg cccactgcgc cagtgtcggc gatattgtca tcatcgccag
    76081 cttcgttacc atgccagatg aagaagctcg cacctggcga cccaacgtcg cctattttga
    76141 aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt
    76201 atccctgcgg ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga
    76261 tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg
    76321 tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaagccgc
    76381 caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg cgacgtgaac
    76441 gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgataac
    76501 catctacccg atgcaaaaaa tgggtatgtg actggaaatc aaatacatcg cgaatttcgt
    76561 taagctcaga aacgctcata aattgcagcg attttggttc ccacttctgc gggtgacgtt
    76621 tgatgcgcga ggtaacaata aacgccgtcg ccttcatgcc atattgtttc aacacaggat
    76681 acgcatagcg gctcactgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag
    76741 gaagattgat cttattcttc acgtaacctt ccagctgcac catgctcagt gtcgcgtatc
    76801 ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag
    76861 tatggcgaaa acgggtgttt tcttcgtcgc gcagaatatg gtgataggtt agcaccggaa
    76921 ggccattatc gggttgggca tccagcgcgc tgatataggc cagtcgatcg ccaatacgga
    76981 tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat
    77041 tgtccgccag taccccaaat ggcgcactgc ccacactcgg cgcgttatag accggcgtat
    77101 ctttccaggt aactaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt
    77161 caactttttg tcgcccctga accggctcaa gatgaccttt atcgataaac cctttatcaa
    77221 agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgtccgg
    77281 cccgaatatt ccccacggtc accatcttgt caccaatttg cgcccagacc gcagcatttt
    77341 cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca
    77401 gcaggagaat aacagcttgt ttgtacatga aaaaatcacc gagggtgaaa caacgctttt
    77461 attgtagcaa aagcgccatc agaatcgcgg gttaatactc atacaaatta tgtttgagga
    77521 cgaaaggcgg atttttttgt tgctggtgcc aaatactact cacctccggc gcacccagtt
    77581 cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc atcatctgtt
    77641 caattaacgg taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg tggcttaata
    77701 atgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac
    77761 cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gaacttaatc
    77821 cgcgccagaa attcacggcc cggcactgaa gaagcgcccc atgttttttc tccagcacat
    77881 ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc
    77941 tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa
    78001 ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg
    78061 ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa
    78121 ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat
    78181 ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga
    78241 gtaaaagacc ccacccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat
    78301 gtcatcgaac gttttggcaa acacaccgtt cagcaaaccc gcaagggaga acaacaccgc
    78361 cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa
    78421 cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac
    78481 atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg
    78541 ctggaacttg gcaccaaaaa atgatgacgc gacgttggcg taggcgttgg tgatcaccga
    78601 catcatgatc agccccggta cgatgaactg catgtagctg aagccatgca tatcgccaat
    78661 acgcgaacca atcaggttac cgaaaataat aaagtaaagg gtcatggtga tgactggcgg
    78721 caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt
    78781 tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa
    78841 acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc
    78901 tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc
    78961 gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt
    79021 gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta
    79081 ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct
    79141 tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag
    79201 ccccacattg agcggcgaag ttcaatatcc acgcctgcag tcggctcgtc gagaatcagt
    79261 agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat
    79321 aacatgcgcg cacgttcgtt gcgttttccc cataaatcga gctgcttaag atatttttcg
    79381 ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt caccacaatt
    79441 tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt
    79501 ttagcgttta cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt
    79561 ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg
    79621 agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg
    79681 ccgcctggat agtttttttt aagctgttga agttccagtg caatggtcat aaatttttac
    79741 ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta
    79801 gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata gatacactca
    79861 tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga
    79921 aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc
    79981 ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta
    80041 acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac
    80101 tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag
    80161 ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt
    80221 tcaaacatag ctcattgctc gacgaaatgc cgcaagagcg ccgtctggat accttgtgtg
    80281 aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg caatcagcgt
    80341 ggaaacgcgg gcagaaagtt accattcacg gctgggctta tggcattcac gacggcttgc
    80401 tgcgtgatct ggatgttact gccaccaacc gcgaaaccct tgagcaacgt taccgtcacg
    80461 ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca
    80521 acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag
    80581 atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga
    80641 gaaaccgata aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt
    80701 acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt
    80761 attccccgag tcgatgatat cttcaacaat cagcacatcc ttgccacgga tatcttcatc
    80821 cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc
    80881 ggtcataaag tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat
    80941 aaatgagcca cgcagcagac ccaccagcac catatcgctg ccgctgtctt tgtaacgctc
    81001 agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac
    81061 ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg
    81121 caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagcaaaa cgattcattc
    81181 gcggaatctg ttaataaagc tcaaattgtg atgacgatca cacatgttaa aacctataat
    81241 taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac
    81301 aggctcgcga cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta
    81361 tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc
    81421 tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg ccgcgctttg
    81481 gctatacgcc gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga
    81541 cttctgggcg ctgactccgc gcagcgatat tctggtcttc ttcggcatct ggctgatcct
    81601 gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt
    81661 ggcactgctg attagcggta gtatcctgac ctgggccgga tttaacgatc cgcaggaaat
    81721 caacggcacc ttaagcgccg atgccacacc tgcggaagct atctcacccg ttgccgatca
    81781 ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat
    81841 taacgccgat aacgtccata atctgaaaga agcctgggtg ttccgtactg gcgatgtgaa
    81901 gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag tgggcgacac
    81961 cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga
    82021 gaaatggcat tacgatcctg agctgaaaac taacgagtct ttccagcacg taacctgccg
    82081 tggcgtctct tatcatgaag ccaaagcaga aacggcttcg ccggaagtga tggcggattg
    82141 cccgcgtcgt atcattcttc cggtcaatga tggtcgcctg attgcgatta acgctgaaaa
    82201 cggcaagctg tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc
    82261 agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg attatcaccg ataaaaccat
    82321 cgtgatggca ggttcagtta ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg
    82381 tggttttgat gtcaacaccg gggagctgct gtgggctttt gatccgggcg cgaaagatcc
    82441 gaacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact cgtgggcacc
    82501 agcggcctat gacgcgaagc tggatctggt gtatctgccg atgggcgtga ccacgccgga
    82561 tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct
    82621 gaatgccaca accgggaaat tggcgtggag ctaccagacc gttcaccacg acctgtggga
    82681 tatggatctt ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc
    82741 agttatttac gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gtaatggcga
    82801 actggtggtt ccggcaccgg aaaaaccggt tccccaaggt gctgccaaag gcgattacgt
    82861 tacccctact caaccattct cggaactgag cttccgtccg acgaaagatt tgagcggtgc
    82921 ggatatgtgg ggagccacca tgtttgacca actggtgtgc cgcgtgatgt tccaccagat
    82981 gcgctatgaa ggcattttca cgccgccatc tgaacagggc acgctggtct tcccgggtaa
    83041 cctgggaatg tttgaatggg gcgggatttc cgttgatcca aatcgtgaag tggcaattgc
    83101 caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtccgg gtaacccgat
    83161 ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc cacagtacgg
    83221 tgtaccgtat ggtgtcacgc tcaacccgtt cctctcacca tttggtctgc catgtaaaca
    83281 gccagcatgg ggttatatct cggcgctgga tctgaaaact aatgaagtgg tgtggaagaa
    83341 acgtattggt acgccgcagg acagtatgcc gttcccgatg cctgtaccgg tgccgttcaa
    83401 tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc
    83461 cgctacggca gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca
    83521 gggtcgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga atggtaagca
    83581 gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg gtgactatat
    83641 tgtggcttat gcgctgccgg atgatgtgaa gtaagacttg ctcagattgc tgacaacgtg
    83701 cgcgttgttc atgccggatg cggcgtgaac gccttatcca gcctacaaaa ttgtgcaaaa
    83761 tcaatggatt gcacaggtaa cgtaggcctg ataagcgtag cgcatcaggc agatttgcgt
    83821 ttgtcatcag tttaatgccc ggagagatcc gggcatattc ccgaatacgg tctttttata
    83881 ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag tgcgccatat
    83941 aagcacgttc tttcggtgcg tcgtgattaa acttcaccag cacttcactg acattgcctt
    84001 ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg ccattttctg
    84061 acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg tcgcccacgc
    84121 cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc ttattcatat
    84181 caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg ccgtggttca
    84241 tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc attccggcca
    84301 tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg agcatcgggt
    84361 ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc
    84421 tacttaatgt gtctggcaaa gcaccggaag cactaatagc aatcggctga atccgcatta
    84481 ccggatgagg cttatcaaac ggcgcaatcg ccatccccat ctggctgacc ggcagcgtca
    84541 ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca
    84601 gcaccggcag ctcgttcacc ttcaccggtt caggtagcag accaccgtcg ctggcaatca
    84661 cataaagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta cagccattga
    84721 gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg cgggtagatt gcaccgttgg
    84781 tcagcaacgt atcgccaaac cagcccacgg cggcggtcat cacatccagt tgataatcaa
    84841 tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca tcgatacccc
    84901 actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc agaccagcca
    84961 gccccatcgc cacctgtcgc ccggtcttgc catgttgatg tggatggaac cagcaggtag
    85021 cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga ataattccct
    85081 gtgggccgcc gtcgacttca cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt
    85141 ccgtcagttg gttatagata tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg
    85201 gccccagcag attgccgtta tagccccagg tagttgcagt tttctcgcca aaggtagact
    85261 gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga
    85321 tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct ccacagtggc aaggctgaag
    85381 ccacacccag tgcgacggaa tatttcaaga aatcacgacg ttgcatagtt atttccttat
    85441 tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta cgggaaggtc
    85501 aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac
    85561 gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa cagtgttatt
    85621 cggcagcctg atggccgttt gcgcaaacag ttacgcgctc agtgagtctg aagccgaaga
    85681 tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt accagaactt
    85741 acctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc agtgggatct
    85801 cagtaattac gacaccttcg acatgaaagc tctcggtgaa gacagctacc gcgatctcag
    85861 cggcattggc attcccgtcg ctaaaaagtg caaggccctg gctcgcgatt ccttaagcct
    85921 gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc gtgcatccgc
    85981 ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa ggaggtatca
    86041 acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg ggcagtactt
    86101 tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga tcatttttga
    86161 gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca ccgagcgcga
    86221 cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa
    86281 acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa
    86341 aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt tctgccgtca
    86401 gtatctaccc aaccataacg ccggtagcta cgacgatccg cgctttaagc tggtgatcga
    86461 cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta tctccgactg
    86521 caccgatcct atcggtcccg gcgaaagcct tttcacttcg gcattttatg aaggctgcaa
    86581 acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca
    86641 ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg ttggctttta
    86701 tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg cgacagataa
    86761 cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg cctctggcct
    86821 gaaatgccgt tattacaatc cggcagtcca tacggcagct tttgccttac cccagtatct
    86881 gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga aaaaactgaa
    86941 actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg atatctgcta
    87001 cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac tctataatgc
    87061 caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcta atattcttaa
    87121 catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg tgagtgaaga
    87181 accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc cagaaacggt
    87241 cgttgcccat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa gtcatcctga
    87301 aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg tgatttctcc
    87361 gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa ccattgatta
    87421 tcgcgtgcgc ggttttaccc gcgacattaa cggtatgaag cactttatcg accatgagat
    87481 taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt
    87541 aaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt tcgaccttaa
    87601 gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc aggaaattac
    87661 cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc cagctgttta
    87721 acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg cacgaaattg
    87781 cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag gctttcttcg
    87841 tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg attggcgcga
    87901 accatcacct cttcgccgtc catccatagc gtatactcat gccctgcccg ctgccaggaa
    87961 cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa ggccaggttt
    88021 tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga catacgcacc
    88081 tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc
    88141 ggcaatgcac cgaaatcgca ttgcgccatt gatgtaatta tcgcagcctt accggagaaa
    88201 aacagcggaa cggcgatggt ttagaaataa aaaaatgccc gggaatctca ttcaccgggc
    88261 attgtgtcgt ttatgcgcag cgcgtgcgct gactttttaa accgcagtct ggaaaatcac
    88321 cccatcggct ttctcggtgt actgagaaag ctggttgaag ttcagataac ggtaagtatc
    88381 aacggctgtt ttatctacct gcgccacgta ggtctggtac tcttccggcg tcggcagttt
    88441 gccaatcagc gccgcaacag cagccagttc cgcagaagcc aggaagacat tcgcgccagt
    88501 acccagacgg ttcgggaagt tacgggtaga ggtggaaacc accgttgcac cgtccgccac
    88561 acgcgcctgg ttacccatac acagggaaca gccagggatc tcgatacgcg caccactctt
    88621 accgaagacg ctgtagtagc cttcttcggt caactgtgcg gcgtccatac gggttggcgg
    88681 tgccacccac aggcgggtcg gcaactgacc tttgtgcgca tccagcagtt tacctgcggc
    88741 acggaagtga ccgatgttag tcatgcaaga accgataaac acttcgtcga tcttctcgcc
    88801 ctgtaccgca gacagcggac gtgcgtcgtc cgggtcgttc ggagcacaga ggattggctc
    88861 tttaatatcc gccagatcga tgtcgatcac tgccgcgtat tccgcatctg catcggcttc
    88921 cagcagctca ggattcgcca gccatttttc catgccctga atacgacgtt ccagggtacg
    88981 acgatcgccg taaccttccg cgatcatcca cttcagcagg acgatgttgg agttcaggta
    89041 ttcgatgatt ggttctttgt tcagcttgat ggtacaaccg gcagcagaac gctcggcgga
    89101 cgcatcggtc agctcaaagg cctgctcaac tttcagatcc ggcagacctt caatttctag
    89161 gatgcggcca gagaagatgt ttttcttgcc tttcttctca acggtcagca gaccttgttt
    89221 gatcgcatac agcggaatgg cgtgtaccag atcgcgcagg gtgatgcccg gctgcatttt
    89281 gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc ctgttgcggc
    89341 tgcaaatgcc accagaccag aacccgccgg gaaagagata ccgatcggga aacgggtatg
    89401 ggagtcacca ccggtaccga cggtatccgg cagcagcata cggttcagcc aggagtggat
    89461 tacgccgtca cccggacgca gcgacacacc gccacggttc ataatgaagt ccggcagcgt
    89521 gtggtgcgtg ttcacgtcaa ctggcttcgg atacgccgcg gtgtggcaga aagactgcat
    89581 caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac gggtcatcgg
    89641 gcctgtggtg tcctgagagc ctacagaagt cattttcggt tcacagtacg cgcccggacg
    89701 aatgcctttc acgccacagg cacggcctac cattttctgc gccagcgaga agccgcgatc
    89761 gctctcagcg acatctttcg cctgacggaa cacatcactg tgcggcagac caagtgcttc
    89821 acgcgctttg gtggtcaggc cacgaccgat aatcagcgga atacggccgc cagcacgcac
    89881 ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc cggtttcgtg
    89941 gttacgtact tcacctttgt acgggtaaac gtcaattacg tcgcccatgt tcaggttaga
    90001 gacatccact tcgattggca gcgcacccgc atcttccatc gtgttaaaga agatgggtgc
    90061 aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg gaatatcatc
    90121 gcccataaac cacagaacgg agttcgtggc ggatttacgc gatgaacccg tacccacaac
    90181 gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga gcttcaatct gtttgatcgg
    90241 gccaacaaca cctggctggt ctggctcaat accttcacgg gcgtttttca gcatcgccag
    90301 cgcgtgcaat gggatatccg ggcgtgacca cgcatccggt gccggagaaa ggtcatcggt
    90361 gttggtttcg ccagtgactt tgaagacggt aacggtcagt ttttcagcca gcgccgggcg
    90421 attcaggaac cattcggcat cagcccagga ctgcataacc tgcttcgcat attcgttgcc
    90481 tgctttcgct ttctcttcta cgtcatagaa gttatcgaac atcagtagtg tgtgagaaag
    90541 tgctttggca gcgatcggtg ccagtttggc atcatccagc gcatcgatca gcggatgaat
    90601 gttgtaacca ccctgcatag tacccagcag ttcgatggct ttttccggag tcagcagtgg
    90661 ggatttggct tcgcctttgg cgacagcagc caggaagcct gctttaacat aggcggcttc
    90721 atcgacgcca ggaggaacac ggttggttaa cagatctaac aggaattctt cttcgcccgc
    90781 gggcgggttt ttcagcagct ctacaagcgc ggccatttgg tttgcatcca ggggtttggg
    90841 cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta gcacgacggt
    90901 tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc tgtgagacag
    90961 cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt ttgacgacgt
    91021 gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag gatggtctag
    91081 agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac ctttctcaac
    91141 gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat tcacagcacc
    91201 tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat gaaaatgact
    91261 ttgccgttta aaccccatgt gctggcgcta atttgcagtg ccgggctttg tgccgcctct
    91321 gccgggctat atataaaaag ccgcacagtg gaagcgcctg tggaaacgca atcgacacaa
    91381 ctggctgtgt ctgacgctgc cgcagttacg cttcctgcaa cggtttccgc acctcccgta
    91441 acacccgccg tcgtcaaatc cgcattcagc actgcacaaa tagatcaatg ggtcgcgccc
    91501 gtcgcgctgt atcccgacgc cctactttcg caggtgctga tggcatcaac ctatccgaca
    91561 aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg cgatgctgct
    91621 attcaggcgg tatctgacca gccgtgggac gccagcgtta aatcactggt ggcctttcca
    91681 caattgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg cgatgctttt
    91741 ctggcccagc cgcaggacgt gatggactcg gtacaacgat tgcggcaact ggcacaacaa
    91801 accggctcgc tgaagtcatc aaccgaacag aaagttatta ccacaacgaa gaaaactgta
    91861 ccggtaacac agacagtcac ggctcccgtc ataccatcca ataccgtttc aactgccaac
    91921 cctgtcatta cagagcctgc aacaaccgtc atttccattg agcccggcaa tcctgatgtg
    91981 gtctatattc ccaactacaa cccaaccgtg gtttacggga actgggccaa tactgcgtat
    92041 ccgccggttt atctgccacc accagccgga gaaccgtttg ttgacagctt tgtacgcgga
    92101 ttcggctata gcatgggcgt tgctaccacg tacgcactat tcagcagcat cgactgggat
    92161 gacgacgatc atgaccatca tcatcatgac gatgataatt atcatcacca cgatggcggt
    92221 catcgtgacg gtaatggctg gcaacataac ggcgacaaca tcaatatcga cgtcaacaat
    92281 ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg gcacaatcca
    92341 aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt tcatcaaact
    92401 gatgtcaacg gcggaatgag tgccacgcag ctacctgctc caacacgcga cagccagcgt
    92461 caggcggcag caagtcagtt tcagcaacga acacacgccg cccccgtcat tacacgagat
    92521 acccaacgtc aggcagcggc acagcggttt aatgaagctg aacactatgg gagctatgac
    92581 gacttccgcg acttcagccg tcgccaacca ctgacccagc aacaaaagga cgccgctcgt
    92641 cagcgttatc agtcagcttc tcctgagcag cgccaggcag ttcacgagaa aatgcagact
    92701 aacccgcaga accagcagcg aagagaggca gcgcgtgagc gcattcagcc cgcctcgcct
    92761 gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cacagatcca gcagcgaaga
    92821 gacgcagcgc gtgagcgtat tcagtcagcc tcgcctgagc agcgccaggt gtttaaggaa
    92881 aaagtacagc agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtgttcaa
    92941 tcagcatcac ctgaacaacg tcaggttttt cgggagaaag ttcaggagag ccgcccacaa
    93001 cgtctaaacg acagtaacca tactgccagg ctgaataacg agcaacggtc agcagtacgc
    93061 gaacgtctct ctgagcgcgg agcaaggcga ctggaaaggt aaattacagg cgtaaaaaaa
    93121 gcggcgtggt tagccgcttt tttaattgcc ggatgttccg gcaaacgaaa aattacttct
    93181 tcttcgcttt cgggttcggc aggtcagtaa tgctaccttc gaacacttct gccgccaggc
    93241 ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac
    93301 aacccatttc aattgccagg ccgatttcac ccagcagctc gccgccgtta gtaccgacaa
    93361 tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg
    93421 cgcagtcgga agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc
    93481 tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg
    93541 tataggcgat ggacgggata actttcggat cgaagtagtg tttcttaccg gcgataactt
    93601 cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccgacgatat
    93661 cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac
    93721 cacggtcgtc aacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac
    93781 gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agctggtgct tttttgcctt
    93841 ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca
    93901 tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg
    93961 ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtaaa
    94021 cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt
    94081 ctttcagttc cagcgcgtca gtagagtccc agatacgcgg atcttcatgc ggaataaacg
    94141 gcagttgaat cgggcgagaa cccgctgcaa tgatcgcgtt gtcgaagttg atcacggttt
    94201 taccgttctc accttcaact tccagggtgt tagccccggt aaatttaccc agaccgttga
    94261 ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca
    94321 ctttctcttt ccaggtacga atcttgtcga tatcggtttt cggttcgccg aagacgatac
    94381 cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag
    94441 aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta
    94501 cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc
    94561 caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc
    94621 cggcgggtct gacgtttttt ttatgacgcc cattatccac cgggtcgttc tatccgtcgg
    94681 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    94741 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    94801 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    94861 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    94921 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    94981 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    95041 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    95101 ctttcttaga aatagtcatc agctcgcgag acagttcgat gatgcctttc ttgttgacgt
    95161 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    95221 atttcttcag ggtcagacgc tgaccgtctt ccgacagcga actgttgaag cgaggcatct
    95281 gctcaagcgc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    95341 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    95401 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    95461 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    95521 ccaccttcgg ccacggcagc atgccaggga taccaccgcc agtcgctgcc ggagctgctt
    95581 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    95641 tacggccagt gcccttcact ttcgcaaggt taacgccaaa ctcgcgtgcc agacggcgga
    95701 tcagcggggt cgcgtgaacg taagcgtcat tttcagcaaa ttcagatttg ccttccgctt
    95761 tcgcagccgg tgctgctgcc ggggcttcag cttttgctgc cggagccggc gctgctgctt
    95821 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    95881 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    95941 gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac tgttcagcgg
    96001 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    96061 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    96121 cctgtttagc cgccggagct gccgcgcctg cttcacccgc cacttcgaag accataatca
    96181 gcgagccggt agacactttg tcgccggtgt tcactttgat ttctttcacg gtgcctgcaa
    96241 acggagccgg aacttccata gaagctttat cgccttctac ggtgatcagc gactgctcag
    96301 cttcaacttt gtcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    96361 tatccggaac gttaacgtct tttgccgcgg cagccgcggg gactgctgcc ggagctgctt
    96421 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    96481 tcataatcag tgcgccggtc tgagttttat cgccaacaga gactttgatc tctttaacga
    96541 tacccgcctg cggagacgga acttccatag aagctttgtc gccttctacg gtgatcagcg
    96601 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    96661 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    96721 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    96781 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc
    96841 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    96901 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    96961 catatagtcg gtagatgcca cagccggagc gtcgttcatc acctgagcga tatacggaac
    97021 gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac catcacgcgc
    97081 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    97141 gatctctgcc gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt
    97201 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    97261 accttccggc attgccggca tgtggtagtt ttcgttcagc gtggtgatgt agtagtaaac
    97321 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    97381 gtaagcgtaa gccgggtcgt aagagataca gttcggaata gtcagcgact gaatgtggct
    97441 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    97501 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    97561 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
    97621 agaggtcgcc gctgccagcc aggaacaacc tgcgcccagc tcgttgatcc cttcctgcag
    97681 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    97741 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    97801 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    97861 cacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    97921 cagcgcgccg aagtcttgca ggcttggcag ctcaagcttc tcggtgaagt tcggctgacg
    97981 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
    98041 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    98101 gaaacggtcg cggatgtgac gcacaccgtc catgttcatt ttcttaacct ggtgcgcgat
    98161 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    98221 cgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    98281 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    98341 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    98401 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    98461 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    98521 accttcgaag ataccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    98581 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    98641 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    98701 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    98761 ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact gccagaattc
    98821 cggcatcaat ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    98881 gttatccagc tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
    98941 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    99001 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatgtggcc
    99061 gcccagttcg aggtcttttt tcgacgcacg cagaaccgtc atgatggcgt tccagcggat
    99121 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    99181 ggggatggtg ttgatgtagt tgctgatacc ggtgcctgcg gctacgttga caccgccttt
    99241 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    99301 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    99361 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    99421 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    99481 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    99541 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctttcttca cggcttctgt
    99601 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    99661 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    99721 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    99781 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    99841 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagttcta
    99901 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    99961 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag
   100021 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
   100081 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
   100141 cctcacgcaa tgagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
   100201 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
   100261 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
   100321 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
   100381 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
   100441 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
   100501 gattttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
   100561 tatgattttt aattcaatta aaacgaattt aaattcattc tgcatattga gaggggttga
   100621 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg
   100681 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaactattc
   100741 aaacttcttt gatgtaaaca aattaataca acaaacggaa ttgcaaactt acacacgcat
   100801 cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca
   100861 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
   100921 cgcgataggg acagggttat tcctgggtag cgcctccgta atacagtccg cagggccagg
   100981 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
   101041 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
   101101 gggcagcttt gctggcttcg cttctggctg gaactactgg gtactgtacg ttttagttgc
   101161 catggcagag ctgactgctg tgggtaaata cattcagttc tggtatccgg aaatcccaac
   101221 ctgggtttct gccgccgtgt tctttgtggt gattaacgcc atcaacctga ccaacgtaac
   101281 agtgtttggt gagatggagt tctggtttgc cattatcaaa gttattgcgg tagtagcgat
   101341 gatcatcttc ggcggctggc tgctgttcag tggtaacggc ggtccgcagg caagcgttag
   101401 caacctgtgg gatcagggcg gtttcctgcc gcacggcttc accgggctgg tgatgatgat
   101461 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
   101521 tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc gcatcctgat
   101581 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
   101641 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
   101701 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
   101761 cagccgtatg ctgtttggtc tggcacaaca gggtaacgcg ccaaaagcgc tggcgtctgt
   101821 cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg
   101881 cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt
   101941 atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc
   102001 caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg
   102061 gatctgcctg ctgtttatgg cggtggtact ggtgattatg ctgatgaccc caggaatggc
   102121 gatttcggta tacctgatcc cggtatggct ggtggtgtta ggtatcggct atctgtttaa
   102181 agagaaaacc gcaaaagccg taaaagcaca ttaatctctc tacgccctca cccgtacagg
   102241 gtgagggcaa taatctttac accaacgccc cgtaaatcgt cagtagcgca atcaccacca
   102301 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat
   102361 cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc tgatattgcg
   102421 aagtacggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
   102481 tcaaggcata taccacacct gcaagacgaa ccggcaccca atccagtaca tgaagcactg
   102541 catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc
   102601 atgcacgcaa aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca
   102661 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
   102721 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
   102781 cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
   102841 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca
   102901 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca
   102961 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
   103021 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
   103081 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa
   103141 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
   103201 gcccgtcatg ttattagcga tatccggata gcaatcaatc agtgcccgcg taaccgccgc
   103261 aagctgttga tactgcgcat cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc
   103321 aatagaaaaa tcattgcagc gttcgcgccc ctgatactga gagactcccg catgccatgc
   103381 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
   103441 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
   103501 agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
   103561 caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac
   103621 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
   103681 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
   103741 tttaacatgc cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc
   103801 attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca
   103861 gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
   103921 acggtgatca cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt
   103981 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
   104041 aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
   104101 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
   104161 gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgtaaaac cttacccggt
   104221 ctgcgttcag cgctgaaata cgcgatactt tgcggcggcg gagcgaatca ccgtctgggg
   104281 ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc agtgcgccag
   104341 gcggtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat
   104401 ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc
   104461 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
   104521 tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc
   104581 tccgtcggtg cgctaactaa gcacgtacag gcactcgacc tttcaatgcg ttttcgctag
   104641 ttgctgacag atttatccgc tccatagtgg gcggataatc tccccttccc caaccttctt
   104701 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaagcag
   104761 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcacca
   104821 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
   104881 ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta
   104941 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
   105001 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
   105061 tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
   105121 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag gttgggataa
   105181 cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca
   105241 gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
   105301 tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga
   105361 agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg cattgcattt
   105421 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
   105481 cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga
   105541 gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
   105601 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
   105661 ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct
   105721 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
   105781 cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
   105841 gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc agccgttaca
   105901 actggcgggc tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
   105961 taccggcagc ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga
   106021 cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac
   106081 gcaaatccat ccgcgtgccg gactcacctt tcagggcgtt ttgcgtgcgt tattgcgcca
   106141 ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat
   106201 taaagccgcg caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga
   106261 aacgctggta cgtttacagc aaatgggggt cgcccgctgg atgctatcat cggcgcttac
   106321 gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc ggcagcaagg
   106381 ggagcccatc cacattccag tcaatgtatg gccgtcgccg ctgccccact ggcaagcacc
   106441 cggttgtgta cattgctacc acggttttta tggtcgcact gccttatttg aagttctgcc
   106501 cataacaccg gtcatacgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac
   106561 gcacgcccga caggcgggta tgcgaacgct ttttgaaaac ggctgcctgg ccgtggagca
   106621 aggcttaacc acctttgaag agttaatccg cgtattgggg atgccgcatg gcgagtaagc
   106681 aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg
   106741 cagagagccg tactttactg cttatggcac tacagcaaca gatggttacc ccactaagcc
   106801 tgaagcgaat tgctatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc
   106861 atcaactggc gacgctactt aaagccgggt taacgctttc tgaagggctg gcactgctgg
   106921 cggaacagca tcccagtaag caatggcaag cgttgctgca atctctggca cacgatctcg
   106981 aacagggcat tgccttttcc aatgccttat taccctggtc agaggtattt ccgccgctct
   107041 atcaggcgat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac
   107101 tggcgcgtca gcaaagagcc cagcgtcagt tgaccgacaa agtaaaatca gcgttacgtt
   107161 atcccatcat cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc
   107221 tgccggagtt tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg
   107281 ggatcatgac gctggcagac tttagtggcg aatggggctg gctgctggtg ttgttcggca
   107341 ttatgctgac gatagccaat aagttgctga tgcgccgccc gacctggctt atcgcgcggc
   107401 agaaattgct gttacgcatc ccgattatgg ggtcactgat gcggggacaa aaactcacgc
   107461 agatctttac gattctggcg ctgactcaaa gtgcaggcat tactttttta cagggcgtag
   107521 agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc
   107581 acgatatcag taacggtcat cccatctggc tggcgttaaa aaatgccggt gagtttagcc
   107641 cgctctgttt gcaattagtg agaacgggag aggcctccgg ctcgctggat ctcatgttag
   107701 acaacatcgc ccatcatcat cgggataaca caatggcgct ggcggataac ctagcagcct
   107761 tactggaacc ggcgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga
   107821 tgtatctgcc aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg
   107881 ttgctccaca ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg
   107941 cacacggata aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt
   108001 acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag
   108061 cggcagctta acggttttac cttctgctgc gcgatattca gcaacgccgc caacgtgacg
   108121 tttcatcgca gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc
   108181 aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc
   108241 ggcaccgcca ccaaaggctt tcgccacatc gcccggcgtg gtgcagccac catcgctgat
   108301 gatcattccg cccagaccgt gcgcagcatc ggcacattcg attaccgcag aaagttgcgg
   108361 ataaccgacg cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt
   108421 aacgatatcg gcacctgaga ggataagctc ctcacacatt tcaccagtca ctacgttacc
   108481 agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg
   108541 ttcggaataa ccattcgcca cgtcaataca aacgaagttt aatgccgggt tcaggtcgag
   108601 aatctgttta gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt
   108661 cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt
   108721 atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtgcctac
   108781 ggtgtccata tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa
   108841 ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg
   108901 gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct
   108961 ggggttaatg gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc
   109021 aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg
   109081 gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cgctcatttt taacgttttg
   109141 ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag
   109201 gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca
   109261 ttgatgccga tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca
   109321 ttgctgatca ctttggcgct aatatgattg ctgctgatgg aacattgcag cgccgggcct
   109381 tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc
   109441 cgctgattca gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt
   109501 gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg
   109561 tggatgtcag cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg
   109621 agcatgtcga acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag
   109681 atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc
   109741 acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac
   109801 ccaggtcctt tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt
   109861 tttgattcag caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt
   109921 cttccgtaat gttagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct
   109981 gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt
   110041 ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat
   110101 ttccgcgccg cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca
   110161 gcgactgagc atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct
   110221 gcatctaccc caggcgcagc gcgactgcca ggtagaaacc tggattgcca gcctgaaccc
   110281 gctcacccag gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa
   110341 acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct
   110401 gaatctgtcg ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc
   110461 cattcgtttt atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga
   110521 actggcctgt tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg
   110581 cgggaaaacg gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg
   110641 tcagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga
   110701 tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc
   110761 acgtaggcct gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg
   110821 cggcgtgaac gccttatccg acatacacac gcttaagctt cgcaattacc ggttcattgg
   110881 ctggcggaaa atcatcggca ttaagaccga ctagcgacat ccacttaccg ggttgccctt
   110941 ctttacccca cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc
   111001 tgtccgggaa ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga
   111061 cttcttcctg aagttcacgc accaccgcct gttccggcgt ttcacccatt tcaattttac
   111121 cgccgggaaa ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga
   111181 tttcattgtt ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa
   111241 acctataaaa aaggcgcaga atcctgcgcc ttttacttca acagttagct tttattgcag
   111301 gcggccatgg cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc
   111361 tactttgcgc tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg
   111421 atggctaagc tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag
   111481 ctcctcaacc tcttcaggca tacgtacctg aactttgctc agcgtactga taacttcata
   111541 tttcaacgac tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt
   111601 cggatctttc tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgctgc
   111661 caggtgctct ttccacaggg agtcaagcgt ttgcagcatg acgcctttct cgaagtgacg
   111721 catcatctca gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag
   111781 aatgcgctca cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcagcaat
   111841 tggtaaatcg aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat
   111901 ttcttccagc gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg
   111961 aatgctgtta atggtttcgc tcacatcgct gacatccagc aattcgttac gctgggagta
   112021 aatggcgcga cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc
   112081 gaagttacga ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg
   112141 ttcaatggct tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga
   112201 agcaaaaata cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc
   112261 cccctgacga ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc
   112321 ggtaccgatg atatgcaggc cacctgctgc cagtaccgca tcgtgacgta cctgccagtc
   112381 ggctttaatt ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg
   112441 ccagctacca ccgagcacaa tatctgtacc acgacccgcc atgttggtcg cgatagtcac
   112501 cgcagccgga taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt
   112561 caggacattg tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc
   112621 gatggagata gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc
   112681 aatgatcgcc tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg
   112741 aatcattgga cggttggtcg gaacaacgac ggtatccagt ttgtagatgg agctaaattc
   112801 gaaagcttcg gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta
   112861 gttctggaag gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt
   112921 cgcttccaca gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt
   112981 gtgttcgtca acgatgataa cttcaccatc tttaacgatg tagtcgacgt cacgggtaaa
   113041 cagcgcatga gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga
   113101 gtacagagac tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac
   113161 cagaccacgt tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc
   113221 gccctggaag gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt
   113281 cacgcgttta tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg
   113341 cgcttcatcg atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg
   113401 tacacgttct tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt
   113461 gttcgtaccg taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc
   113521 cggcaggttg ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc
   113581 gtcacgttgc gccaggtagt cgttgacggt aactacatgc acgcctttac cggttagtgc
   113641 gttcagataa gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc
   113701 gatgcagcgt tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc
   113761 aaagacgcgc ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag
   113821 cacttcgcct ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc
   113881 ggagagtttt tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg
   113941 gcgcagggtg cgatcgttac gactaccgaa aactttagtt aataatttga ttagcataat
   114001 aaaatctcaa acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt
   114061 agctgaggcg ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc
   114121 tgaattttgc ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg
   114181 tgccttcctg ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa
   114241 gaggcaaaga ttcttcagca acgggcagcg tttgcggtgc cattgcgaag gaaagatgac
   114301 ggattaccgt gcgaatggca tgttgatgcc agtaatctac ggaatagttc gaattcgggc
   114361 ggcgcgtgtt cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt
   114421 ggttgcgggt tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca
   114481 aacctaaact cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa
   114541 actgtcgcca gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg
   114601 tatttagcgc acaaatctta tcattaaagc ctgaagtcca cagcacgaat gaagataagt
   114661 tgtccgtaaa agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat
   114721 tcaaaaaaaa acgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt
   114781 tatacgacag tataaatgtc gttatgccag tacggctgaa ggcgctttga aggccaacgg
   114841 cagttctgcg tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc
   114901 ctgcagcagt ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat
   114961 attgtgacca cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc
   115021 gtcttcaaaa cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca
   115081 atcgaagctg ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa
   115141 accgaacgta cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc
   115201 atagcgctgg ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga
   115261 aaaaccattg tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc
   115321 tttgatgcga acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag
   115381 gtatacaaac ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat
   115441 aacaatgtta tcgatgccca agcccgcgag agcagcattg aggtgctcta cggttgaaat
   115501 ccgtacatca tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc
   115561 atcggccggg aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt
   115621 ggccggcgca gggcgtaacg tcagggtgac tttcttgccg gtatgtaaac cgacacccgt
   115681 cgcctgaacg atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac
   115741 ctatccaacc gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa
   115801 cccaaattcc agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat
   115861 aatccggctc tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct
   115921 gctcctgggt cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa
   115981 cctgcttatt ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa
   116041 caacggttac gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag
   116101 tcgcgttgtc ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac
   116161 gcaggtcgaa gcccgctgtg atgttaacca gcacgccgcg tgcgccagac aggtcgatat
   116221 cttccagcag cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc
   116281 tcgccacgcc agaacccatc attgcgtagc ccatttcaga cattacggtg cgtacgtctg
   116341 caaagtccac gttcatcaaa cccggacgag taatcagttc ggcgatgcct tgcacagcgc
   116401 ctttgagtac atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa
   116461 ctttcagcag tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag
   116521 tgatcccctg ctccgcgaat gccatacgct tcttgccttc aaagttgaag ggcttagtga
   116581 cgacagcaac ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac
   116641 ctgtaccggt accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg
   116701 ccgcacgcag tgcatcgcga tcctcatctg ccgcattgcg gccaacttct ggattagcgc
   116761 cagcgcccag tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt
   116821 tacgcagcgc ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atgcgctcgc
   116881 gcaccatgtg ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg
   116941 cgtcattggt aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct
   117001 gaggccgtaa tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga
   117061 gtcgcttgat ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt
   117121 taagatgtga ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct
   117181 gagcataatc cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa
   117241 acacgcgctg agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa
   117301 tgcctgccgc caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata
   117361 tctcttcgtt gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg
   117421 tctgacgttg cagactccgc ggcggacgac cgcctacgct cggcacttcc acgctctcat
   117481 cttttccaac gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc
   117541 ttggcggcgt gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa
   117601 ttaccttagt gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac
   117661 cgatatcgac gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg
   117721 atgccagacc ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga
   117781 cgatgttttt cgccatatcg ttgtggcatg tgatcaggtg cacttttgcc tgcatccgca
   117841 cgcccgaaag ccctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg
   117901 ggatcacatg cagcacacga tgctcatcgc gtacacgcac cgatttcgcg gtatggacga
   117961 cgttttccac atcttcttgc gttacttctt cttcagaaat aggcaccata ccaatttcat
   118021 tctggcagct gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg
   118081 ccatcaattc tgcctggtca atggcacgtt gtacgcactt gaccacggat tcgaggtcgt
   118141 tcaccccgcc tttatccata ccacgcgacg ggcagctgcc cacgccaatg atattgacca
   118201 taccgtcggg cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc
   118261 ctactaccag ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg
   118321 ttgctgagta gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata
   118381 acgcaaatca acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtagaaccgg
   118441 ataaagttct acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt
   118501 aatatcgtta ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag
   118561 agtaaatctg tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt
   118621 ggcgctgcct tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg
   118681 cacgctgaag gtatttcctt ccgcgtctac catgtgttga tcattccacc gcgcaatcgg
   118741 cacatattca accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg
   118801 cttaatccac ggcagacgtt gttctatttg cgtctggatg atgttgacat cctgggtcat
   118861 aaaggtaccc ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta
   118921 atggcgttca ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca
   118981 gcccaacacg acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat
   119041 ccccgccaga cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tgtttcgcgt
   119101 gttcagagca gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg
   119161 agaagctcat acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg
   119221 gtgaggtatt ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac
   119281 gtccccatcc tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg
   119341 cctcttgtga cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact
   119401 tcgcctcata atcatagaag gttccggacg gttgaatacg tactgacggt aaaatttctt
   119461 caccgagtat cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt
   119521 catcgtgctg aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg
   119581 acattcccac actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag
   119641 aaatttctgc taactgctta tcactcaggc ctttttcaaa ctctacgcgg gttaacgcta
   119701 cccacggcgc gaccggtaaa ccggcacctt gccacagaag tttgctgcgt agtttatcca
   119761 ttgaaagcgc agatgccatc actccgctgc cggtataagg caagcccatc agctcgagca
   119821 tcccctgcag cgtaccatct tcaccgccgc ggccgtgtag cgcgataaac actttctgaa
   119881 agcccatcga cttcagttgc gtcacgtcaa cttctttcgg gtcgacagga tatgcgtcaa
   119941 taccgccttc acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct
   120001 cagcggaagt cccgcccaac aggaccgcga ttttatcagt catgttgttc ttcctccgga
   120061 gtttgcggct tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc
   120121 ccctgaacga gaatcaggtc gttaccggtt aataccggtg ccagcatctc ggctacctgc
   120181 gcagggtccg gcaccagaat gggatctatt ttcccacgtc cacgaattgt gcgacacagc
   120241 gaacggctgt ccgcacccgg aattggcgct tcgccagccg gatacacttc cagcatcaac
   120301 agggtatcaa cctgcgtcag cacattggcg aaatcatcat acaggtcgcg tgtacgggta
   120361 aaacggtgcg gctgaaacag cattaccagg tttttatccg gccagcctgc gcgcgccgct
   120421 ttaatggtgg cgtccacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta
   120481 ccgcttttac cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca
   120541 gtcccctgga agctctccag cgcacgcaaa atagcctcgt cgtcaatgcc ctcttccgta
   120601 gcaaccgcaa ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg
   120661 acgcgcatcg gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc
   120721 tgataatctt ctacacgcac gtcggcatct tcgctgaagc cgtaagttgt ggtctgacgt
   120781 cccactcgcg gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg
   120841 taaaacggca ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc
   120901 tggtaggtat ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga
   120961 tgcaggaacg atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa
   121021 cgcgcatgaa cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc
   121081 gcttctgcgt agatgctgga gaccatcgcg gtagttgtcg ttttgccgtg cgttcctgca
   121141 atggcgatgc catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc
   121201 ggaatacgcg cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa
   121261 acaacgacca cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct
   121321 cccagattca ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga
   121381 taaccttcat tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca
   121441 aaatgtatgt gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt
   121501 tgtgtattca ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct
   121561 acaattacgc ccgggcagcc cgactcactt catttgccac tcgctcggtg gcatccggaa
   121621 tggatgcagc gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca
   121681 gggtgttggc gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg
   121741 ctttttccag cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa
   121801 acaacgccgg tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa
   121861 cgacgacatc cgcccacgca tacgccgccg ccatatcatc aataaattca gtcgctttat
   121921 gctgcggttg ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttgccgc
   121981 tctgatgcca gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt
   122041 taagaatgcg tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc
   122101 cagccaaacg ttgctgcggc aacggtagcg ccaacacatc ggtacgcacc gggttaccca
   122161 ccacttccgc attggggaaa gcacctggaa acgcctgcat cactttggtg gcaatcttcg
   122221 ccagccattt attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc
   122281 ctaacgacca cgcggccaga ccaccaggac ctgatacgta gccgcccata ccgagcacca
   122341 cgtcaggttt gtacgctttc ataatcgccc gcgcctgacg ccaggcattg aagatacgca
   122401 gcggcgcagc tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat
   122461 caatttcgat gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc
   122521 agcgaacttg ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat
   122581 gtccaccggt tccgcctgcc atcaccatta atcgctttcc ttgagcactc atcgtgaacc
   122641 tcgtacaaac gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat
   122701 gatggctgtc gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag
   122761 acctttggtc ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa
   122821 ccagatgcca atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaatgcttt
   122881 acggccaatc gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac
   122941 accgacatac cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg
   123001 cagatactcc agtttttgta cagagttacc taaaccttgt ccccacagtt cgccgcgacc
   123061 aaacgccatc agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt
   123121 ccagaatgcg gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc
   123181 tgaaatgccc ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa
   123241 catcgccagc gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag
   123301 taacactgcc aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg
   123361 tacttcgtca cctttacgca ccagatagtt ggcgatatag caaaacagcg acagttttgt
   123421 cagctcagca ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac
   123481 cgagctcccc actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc
   123541 actgtagcgt tgccagaact ccatcggcag acgcagcgta atgatcgcca gaataaacgc
   123601 caaaatcaga tagacaccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg
   123661 ccctatgggc attgacgccg aggtcaccat gataaagcca atcgccgcga ggccgaaggt
   123721 cagccacagt aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc
   123781 catcacccag ccctttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg
   123841 cggcattttc aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca
   123901 aactcattgc ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag
   123961 agtagaacca tatcgcccgg ctgaacacgc ggagccagca agcgcatcgc ctgttccata
   124021 gtttcggttt gttctgccac ttccgggcgt agcgccgcca gctgcacgcc gtcacgaccg
   124081 aaacaataca gacgtacgtt atcgccattc aggtaacgcg ccagtgggct aaagtccgcc
   124141 gatttaccat cgccgcccag caacaaatgc agcgtgccgt ctacgtgcag gccattcagc
   124201 gccgcttccg tactgccgac gttggtcgct ttcgaatcgt taatccaacg tacgccgtta
   124261 tgctccagca caacttcaaa gcgatgcggc agaccagtga atgtggttaa cgctttcagg
   124321 ctgctggcac gcggtaaccc tgcggcatct gccagcgcca gcgccgccag cgcattggtg
   124381 tagttatgct gcccggaaag tttcatttct ttcacgttca gcactttctc gcctttcacc
   124441 cgcagccagg tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag
   124501 ctgacgcagc gttcatccgc accgcgaatc ggcattgtta aggcatcatc agcattgacg
   124561 acacaaactt tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgtaaacca
   124621 aacggatagc gatccatatg atcttcagtc acgttcagaa tggtcgctgc taccgcctgt
   124681 aagctggagg tggtttccag ctggaagctc gacagttcca gtacgtacag ttcgcactca
   124741 tcatccagca gcatcaacgc gggcaggcca atattgccac ccacaccaac gttaaccccc
   124801 gccgctttcg ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta
   124861 atcgccacaa tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg
   124921 attccggcat cagcggcagc gcttaaggat ggatgcgcca gtgcaatacc gggactggcg
   124981 acaatcagat ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg
   125041 gcttcgggta atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc
   125101 acaccgcgag cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg
   125161 acaacatttt taccctggta atcagccatg attaacgtac cttcagcgtt gccagaccaa
   125221 tcaggaccag catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc
   125281 ctttcagttc atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt
   125341 taaaggagcc gacctgcagg atgacggaaa gcgtttctac cacgaacacg ccccccataa
   125401 tcaccagcag gaattcctga cgcagcagta cggcgataat gcctaacgca ccacctaacg
   125461 ccagcgaacc tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc
   125521 ccagccctgc cccgactatc gcggtacaga caataaccag ttccccggcg tgtcgcagat
   125581 atggtatgtg taaatagctg gcaaagttca tgttgccggt cgcccacgcc accagcgcaa
   125641 aaccaccggc gacaaatacg gtcggcataa ttgccagacc gtcaagacca tcggtcaggt
   125701 ttaccgcgtt gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca
   125761 gttgcggcat cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc
   125821 caacaaggta cagcgcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc
   125881 aacgagcgat caaccctttg gtgtctttac gcactacttt gcgataatca tcaacaaagc
   125941 caataacacc gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt
   126001 aagcccacag cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg
   126061 gcgtaccgcg cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg
   126121 aaagtttttg caaatgagca atcatacgcg ggcccatcca caatgagatg aacagcgcgg
   126181 tcagcaggct gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat
   126241 atttgaccaa atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac
   126301 tacctcttcc atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc
   126361 agcaatcagt gatttaagac gcgtaattaa cgcagttttc tcagcaaaat gttcgccaac
   126421 gccgctggcg gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc
   126481 agcagctttt gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc
   126541 cgccatatcg cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc
   126601 ggcggtcatt gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc
   126661 cagttgaatg gggaacaaac ggcctggaac agctttcaga tttgccagcc ccgctttgat
   126721 agcatcaagc gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat
   126781 attgtgacgc cccggcaacg gcagcagaac atccacgcta ccggttgggg tttgcagggt
   126841 aaattccgta ccgtgcgaag tcacatggat attggtggcg gtgaaatcgc tgttggcggc
   126901 attaggtgag aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc
   126961 gttgttgtcg gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc
   127021 tttcgctttc gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt
   127081 gttgaccagc gcagcttccg ggcgagtcag actcacagtc cagacaattt cgccctgatg
   127141 gttcgcgcca agttcaataa ctgcgtaatc gtattccggc gttaagcgca acagcgtcat
   127201 cggtacaccg atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct
   127261 taaaatcgcc gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac
   127321 cacgcgcgcc ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt
   127381 atccttgacg attaactgcg gcaggtcgat atccagcgga cggctaacca gtagtgcgcc
   127441 tgcgccgcca gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag
   127501 ggcaacaaac aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt
   127561 gatatctgca ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct
   127621 aatcatgcaa tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc
   127681 tgattgccaa caatctggta atcttcatgg cctttgcccg cgaccagcac cacatcgttc
   127741 tctttagcct gcataacggc gcaagtcacc gcttcagcac ggtcttccat cactttggca
   127801 tgtccggcat ctaacattcc cgccagaata tcgttgatga tggcacgcgg ctcttcggta
   127861 cgcggattat cgtccgtcac caccgccaca tcagcaaact cttcggcaat tgcgcccatc
   127921 agtggacgct tacctttatc gcgatcgcca ccacagccaa agacacacca cagcttgccc
   127981 gcacagtgca gacgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa
   128041 tccaccacca ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt
   128101 tgcagacgag cagcggtttt cagcagatca gccagtggat agcccagcgc cagcagcgtc
   128161 gccagcgcga gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg
   128221 ccatcgcccc aacttgagct aaagcgaata gtcgcaccgc tgtcgtgata gttcacttcg
   128281 gtcgctttca accagcgtcc gtgacagttc ggattgatat gatcttccat tgataccgca
   128341 actgcatccg gcagttttgc cagccagcgg cggcctactt catcgtcggc attaataatc
   128401 gcctgaccgc aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata
   128461 tcaccatgat aatcaaggtg atcgcggctt aagttagtaa agaccgacgc cgcaaatttc
   128521 aatgccgcca cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaaagtc
   128581 gcgtcctgat ccaccagccc cgccagctca tgctgaacat caactgccga accggtggta
   128641 ttttctgtcg ggatcacttt ccccagcaga ccgttaccaa cggtgcccat taccgcgctg
   128701 gtttcgccaa gcagttggct ccactgcgcc agcagctggg tcgtcgtggt tttgccgttg
   128761 gtgcccgtta cgcccacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc
   128821 agtgcagata aacgctcgtt gagctggctg agatagatga ccggtacgcc gtgcatttca
   128881 cggatttcac cgtcggtcgc ctcacctttc gcctctgcaa taatggcagc cacaccttgc
   128941 gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac agctacaaag
   129001 agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt
   129061 gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac ctgtcccctc
   129121 gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg gctcgatgtt
   129181 catggttcgc aatacgccgc ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc
   129241 gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc gcggctgact
   129301 cgcaggcgca acacctgcgg tgtaagcaat atatttattg atgtagcgac cgtccggccc
   129361 gacctttttc gcggtaccgg ttttaatggc aatacgatag cctttaatcg ccgccttcac
   129421 gccgccgcca cccggtagcg ccacgctttc catcatatgc accacagtgc ggacaataga
   129481 ttccgggaag acacgttcac cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg
   129541 ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg gagttaccat
   129601 tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt gtttttgagg
   129661 atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca gtccaaaacg
   129721 tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagcttgg aaacaccgac
   129781 gttactcgac ttctgtaata ctccggtcag ggttaattcg ctgtagcgtg ccacgtcttt
   129841 gatttcgtgg ccgttaattc gataaggaat ggtattgagt accgagtttt cccgcaccac
   129901 gccacgttgc aacgcggtca tcaccaccat cggtttaacc gttgaacccg gttcaaacac
   129961 gtcggtgatg gtacggttac gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt
   130021 gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca ccagcacggc
   130081 gctaccggac tcagccttgt taaaggccac cgcgttgttc agttcgcgat aaaccagcgc
   130141 ctgcaggcgt tcatcaatac tcagcgccag gttgtgcgca gcctggctgt cagtagaaga
   130201 aatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac ccggctgccc
   130261 ggtaagccat ttatcgaaac tcttctcaac gccctcaata ccttgactat cgacgttagt
   130321 aaagccgatg aggtgagcag tcacttcgcc ggatggataa taacggcgag attcttcacg
   130381 cagatgaatt cccggcagtt tcagtttttt gatgtagtcc gccatgtcag ggtttacctg
   130441 acgcgccaga taaataaagc gccctttcgg gttggcgtta atgcgggcag aaagctgatc
   130501 cagcggaata ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc tgataccgcc
   130561 agcgtcatgc acttctttcg ggtcagccca aatcgctttt accggcacgc tcaccgctaa
   130621 cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag
   130681 agaacgcatg tcgccctctt tcaccagcat atccggggag ataacttgta accacgctac
   130741 gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg caaaacgcca
   130801 actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt
   130861 catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg gatcaacatg
   130921 ctgcatttgc agcttttccg tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt
   130981 ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca gcaccagttg
   131041 ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt
   131101 caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc
   131161 gataacacca ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag
   131221 agcttctgtc actctgctga tcatgcgttc gtcctctctg caatacgcag aactgaacta
   131281 cgggcacgag gattctcagc cacctcttct tcgcccggca ttaatttgcc tagtgctcgc
   131341 agctgacggc cacccagttt tttgagctgc tcttcagtca tcggtaaccc tgccggaact
   131401 tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg
   131461 aagctgatga tcgaaagccg cccacccggg gccagcacgt tgagcgagct ttttagcgcc
   131521 tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa ggtacgggtc
   131581 gcgggatgtt taaacttatc tttcaccggc gttgcagcag ccacgacttc cgccagttct
   131641 ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggctcgggc aatgcgtttg
   131701 gcaaaacgct cttcaccata ggttttcaat acccaggcga tatcggcttc ttctgcggtt
   131761 tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc cagcggacca
   131821 tcgcgcataa aggaaaagcc gcgttcagca tcatcaagtt gcggtgaaga gacaccaaga
   131881 tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta ttcgcccagc
   131941 gcggaaaaag gtccgtggat gatggagaag cgcggatcat caatagtctt cgcaacggcg
   132001 atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag ctgcgagagg
   132061 atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg
   132121 atattgaggc cattaacggc ttcatccagc agcaccgtag tatgtttata gttttccatc
   132181 attttataga gacaagtcct gcagtcgctc cgataagtct ccggtagcca actgctctgc
   132241 gtcgatatct tccttgacct gttgatgcca ggttgtttca tcccacagct caaacttgtt
   132301 gaactgtcca accagcatca cttcttttgt cagcccggca tgttgccgca gtactggcgc
   132361 gattaacaat cgacctgcgc catccatctg acattcgctg gcatgaccta acagtaggcg
   132421 ctgcacacgg cgctcaaccg ggttcatgct cgacagacgc gataattttt gctcgataat
   132481 ttcccattca ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac
   132541 catttgaccg gcagcgttct caagcagctg ttcccgataa cgggtaggca ctgataagcg
   132601 ccctttgctg tcgagattga ctaacgttgc tccccggaac atgccagcct caccccttat
   132661 tcccactttg ccccacaatt tcccactgaa aggagtttac ggagcggagg aaaagcttgt
   132721 caagccagtc acaaggcata ccgaggtaaa aaacccgatg tttacggcta atatcagcgt
   132781 tgtttatttt acgtccaacg tgcgaagcaa attaacgtta tgaatagctg taagaaaaaa
   132841 accctcatcg tcatcaccgt tttaaaatga ctcactattt tccacagcaa atataaagtg
   132901 tcagtttgcg acgcgagcgg cattttagga catatcttcc ccggttaaca gtccctgttg
   132961 cgtctgtgta gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa
   133021 atcggcaatt catctctgca tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt
   133081 aattattgtg aatttcagct ggtgagttaa ttaactggcg ggtttaaaag catttatcaa
   133141 taatagtcag gaatagtctt atttacttta aggatattga tgtctagtcc cgtactctac
   133201 gcgccagagt gaaattcacc tggcgcgtat ttttgttcgc agcttagcta cggctgagca
   133261 caccgcggcg atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt
   133321 cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag
   133381 ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa
   133441 ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt gacatccatc accccttgca
   133501 acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc agccattttt
   133561 cgaataactg ggcagccgcc tcccgctcat agctgttggc atacaggaaa tgcacttcgc
   133621 gcggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct
   133681 ccggcagcgc gccaagataa agcaccgtct cggcgggaaa cttacgtaac tcttccgcca
   133741 gcatttcggc atcatcctga tcggcaccaa ccacgctggt aaagtgttca cgatcgaggg
   133801 cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct
   133861 caggaggcaa cgacgtcgaa acaataatgg catcgacctg acgctgtaaa aggtgctcaa
   133921 tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc aatcagcagt tgataacccc
   133981 gttgccgcgc ctggcgttca agatagttag cgatgcgggt atagctggtg ttctccagat
   134041 cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt
   134101 tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt ttgtcactca
   134161 cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg
   134221 ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata
   134281 gccattgtac tggtatgggt taaattcatt aacatctaag cgcagaaaat taactgcggc
   134341 aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg
   134401 gctgtaaatt gcgcattgag atcattctca acgcattatt ttatcgccgc gcgaaagtcc
   134461 gaccacacca gagcgagcaa cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa
   134521 tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc
   134581 aataatttgc ccacggaata tttccgtatt acgtttaact tcgtcacgcc cgtaaccgct
   134641 ggcctgaatt ttcaccagca tgatttccct ctcaacatgc gcgccctgcc ccaactcact
   134701 cacgcgcaag acatcgacca gcttgtataa ttgcttttcg atctgctcaa gcactttttc
   134761 atcgcccacg gtctggatgg tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt
   134821 caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc
   134881 tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg
   134941 cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcactg
   135001 ccatcgacgg tgacatcaac aaataccagg cgattattac gcacctgttc aagcgcctcg
   135061 ctaagtttgc tttccagctc ttgcggatga gagatctgga tcccgacgtg accataggct
   135121 tccgccagac ggacgaagtc tggtaaagac tgcatataag attgtgaatg acgaccggaa
   135181 tagatcatgt cctgccactg cttcaccatc cccaaatagc ggttattaag attaaccacc
   135241 agcaccggca gttcatattg caacgctgta gacagttcct gaatgttcat ctgaatactg
   135301 ccgtcgccag tgacgcaaac cacggtttct tctggcagcg ccattttgac gcccagtgcc
   135361 gcaggtaaac caaagcccat agtgccgagg ccaccggagt tgatccaacg acggggtttg
   135421 tcgaatggat aataaagcgc agcaaacatc tggtgctgcc cgacatcgga cgtaacgtaa
   135481 gcgcccccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
   135541 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatctg ctgccaccag
   135601 tcgcggatct catccagcgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt
   135661 tcgaggacct ggcgagcatc ccccacaatc ggaatatccg cagtcacggt tttagaaatg
   135721 gaagtaggat caatatcgat atgcagaaca gtggcatttg ggcagtactt tgccagattg
   135781 ttagtggtgc gatcgtcaaa gcggacaccg acagcaaaaa tcacatccgc gttatgcatc
   135841 gtcatattgg cttcgtaggt accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt
   135901 gccggaaacg ctcccagccc catcaatgag gaaacaacgg gcagatttaa cgcctccacc
   135961 gtttctttca gctgttgatg gcagcccgcc gtgattgccc caccgcctac gtagacaacc
   136021 ggtttttttg ccgccaccag cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta
   136081 gtagtgggat tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc
   136141 gccggattaa gaatatcttt cggtaaatca acgaccactg gtcctgggcg accacttgcc
   136201 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
   136261 ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa
   136321 cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa
   136381 gcggtggcga tgccggtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
   136441 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga
   136501 cgaactaaaa catgaccgat accacccacg gtatgcaatg catcgtaaat atcaaggact
   136561 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
   136621 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
   136681 aaacacatta accgtccgat cttgcttaag caattggcag ccagagacat aaacgccggg
   136741 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga
   136801 taaaaatgat aataaatcgg ctaaatccca ccacttacag caatttctct atttttttaa
   136861 ttaatcctaa aatagagagc ccacacattc agaataaaat tctacactca ataaaaaatg
   136921 cagaataaac cagacattca tgtctgacct attctgcatt cagttagcgt ttgcaaattg
   136981 agactaattg ctcttccatc cactgatggc ctttatcacg cccggcagct tcatgccagg
   137041 agagataaca ggttctgcta tttagtttta acggcagcgg taatacctgt aattctaagg
   137101 attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca
   137161 ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta
   137221 ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt
   137281 gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttgggtga tttttgctgg
   137341 ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt
   137401 cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat
   137461 ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa
   137521 tctgcgaggt cagaatactg tctaacgggc tgcaaacaca aagatgaaat acacgttcac
   137581 tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac
   137641 gaactgaacc aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa
   137701 caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt
   137761 gcgacattcc cagaacatga gcagcacggg taatgttttg ctcctgcatc acggcatcga
   137821 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgctgaac tccgccgtct
   137881 ctggatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt
   137941 cccttattgg aatgcagaga aataatcctc gagaatatga tttatcatgc atataaaata
   138001 agaaaaagga aaatgagtaa aattaggaaa acataaagat acgggttctt ataaaaacag
   138061 aatccattgc cgtggaaagc gtcatttacc acagcataat aatccataat gttcttgttg
   138121 cgtaaacaat ctaatcataa ctaccgcgaa tactcaatca tccacaaaa
//
LOCUS       DAZGXS010000002       118581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGXS010000002 DAZGXS010000000
VERSION     DAZGXS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 118581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(76..1341)
                     /locus_tag="ACHKG5_000126"
     CDS             complement(76..1341)
                     /locus_tag="ACHKG5_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0866319.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHKG5_000127"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHKG5_000127"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKG5_000128"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKG5_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0866320.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHKG5_000129"
     CDS             2580..2663
                     /locus_tag="ACHKG5_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866321.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHKG5_000130"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHKG5_000130"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0866322.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKG5_000131"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKG5_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0866323.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKG5_000132"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKG5_000132"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0866324.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKG5_000133"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKG5_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0866325.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKG5_000134"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKG5_000134"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0866326.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKG5_000135"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKG5_000135"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0866327.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKG5_000136"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKG5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0866328.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKG5_000137"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKG5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP0866329.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKG5_000138"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKG5_000138"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0866330.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKG5_000139"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKG5_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0866331.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHKG5_000140"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHKG5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0866332.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHKG5_000141"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHKG5_000141"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0866333.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHKG5_000142"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHKG5_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0866334.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHKG5_000143"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHKG5_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0866335.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHKG5_000144"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHKG5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0866336.1"
                     /translation="MNDIAHNLAQVRDKISAATTRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="ACHKG5_000145"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="ACHKG5_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0866337.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHKG5_000146"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHKG5_000146"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0866338.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHKG5_000147"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHKG5_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0866339.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHKG5_000148"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHKG5_000148"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0866340.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHKG5_000149"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHKG5_000149"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0866341.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHKG5_000150"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHKG5_000150"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0866342.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHKG5_000151"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHKG5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0866343.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHKG5_000152"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHKG5_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP0866344.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ACHKG5_000153"
     CDS             complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ACHKG5_000153"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0866345.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25438..25689
                     /gene="yqgD"
                     /locus_tag="ACHKG5_000154"
     CDS             25438..25689
                     /gene="yqgD"
                     /locus_tag="ACHKG5_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0866346.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25800..25943
                     /gene="yqgG"
                     /locus_tag="ACHKG5_000155"
     CDS             25800..25943
                     /gene="yqgG"
                     /locus_tag="ACHKG5_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0866347.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26037..26168
                     /gene="yqgB"
                     /locus_tag="ACHKG5_000156"
     CDS             26037..26168
                     /gene="yqgB"
                     /locus_tag="ACHKG5_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0866348.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26177..28153
                     /gene="speA"
                     /locus_tag="ACHKG5_000157"
     CDS             26177..28153
                     /gene="speA"
                     /locus_tag="ACHKG5_000157"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0866349.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            28299..29030
                     /locus_tag="ACHKG5_000158"
     CDS             28299..29030
                     /locus_tag="ACHKG5_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0866350.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            29166..30086
                     /gene="speB"
                     /locus_tag="ACHKG5_000159"
     CDS             29166..30086
                     /gene="speB"
                     /locus_tag="ACHKG5_000159"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0866351.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(30292..31050)
                     /gene="loiP"
                     /locus_tag="ACHKG5_000160"
                     /gene_synonym="yggG"
     CDS             complement(30292..31050)
                     /gene="loiP"
                     /locus_tag="ACHKG5_000160"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0866352.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            31328..33319
                     /gene="tkt"
                     /locus_tag="ACHKG5_000161"
     CDS             31328..33319
                     /gene="tkt"
                     /locus_tag="ACHKG5_000161"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0866353.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            33633..34076
                     /gene="cmtB"
                     /locus_tag="ACHKG5_000162"
     CDS             33633..34076
                     /gene="cmtB"
                     /locus_tag="ACHKG5_000162"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0866354.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            34104..35492
                     /gene="cmtA"
                     /locus_tag="ACHKG5_000163"
     CDS             34104..35492
                     /gene="cmtA"
                     /locus_tag="ACHKG5_000163"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0866355.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            35507..36784
                     /gene="yggP"
                     /locus_tag="ACHKG5_000164"
     CDS             35507..36784
                     /gene="yggP"
                     /locus_tag="ACHKG5_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0866356.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            36781..37746
                     /gene="glpX"
                     /locus_tag="ACHKG5_000165"
     CDS             36781..37746
                     /gene="glpX"
                     /locus_tag="ACHKG5_000165"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0866357.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            37768..38277
                     /gene="fumE"
                     /locus_tag="ACHKG5_000166"
     CDS             37768..38277
                     /gene="fumE"
                     /locus_tag="ACHKG5_000166"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0866358.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            38274..38987
                     /locus_tag="ACHKG5_000167"
     CDS             38274..38987
                     /locus_tag="ACHKG5_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0866359.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(38959..39636)
                     /locus_tag="ACHKG5_000168"
     CDS             complement(38959..39636)
                     /locus_tag="ACHKG5_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0866360.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(39630..40307)
                     /locus_tag="ACHKG5_000169"
     CDS             complement(39630..40307)
                     /locus_tag="ACHKG5_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0866361.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(40295..41002)
                     /locus_tag="ACHKG5_000170"
     CDS             complement(40295..41002)
                     /locus_tag="ACHKG5_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP0866362.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(41003..41581)
                     /locus_tag="ACHKG5_000171"
     CDS             complement(41003..41581)
                     /locus_tag="ACHKG5_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP0866363.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(41604..42035)
                     /locus_tag="ACHKG5_000172"
     CDS             complement(41604..42035)
                     /locus_tag="ACHKG5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP0866364.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            42407..43426
                     /gene="epd"
                     /locus_tag="ACHKG5_000173"
     CDS             42407..43426
                     /gene="epd"
                     /locus_tag="ACHKG5_000173"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0866365.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            43476..44639
                     /gene="pgk"
                     /locus_tag="ACHKG5_000174"
     CDS             43476..44639
                     /gene="pgk"
                     /locus_tag="ACHKG5_000174"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0866366.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            44854..45933
                     /gene="fbaA"
                     /locus_tag="ACHKG5_000175"
     CDS             44854..45933
                     /gene="fbaA"
                     /locus_tag="ACHKG5_000175"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0866367.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(45997..46095)
                     /locus_tag="ACHKG5_000176"
                     /pseudo
     CDS             complement(45997..46095)
                     /locus_tag="ACHKG5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46291..47151
                     /gene="mscS"
                     /locus_tag="ACHKG5_000177"
     CDS             46291..47151
                     /gene="mscS"
                     /locus_tag="ACHKG5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0866368.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            47290..47925
                     /gene="argO"
                     /locus_tag="ACHKG5_000178"
     CDS             47290..47925
                     /gene="argO"
                     /locus_tag="ACHKG5_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0866369.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            48018..48758
                     /gene="yggE"
                     /locus_tag="ACHKG5_000179"
     CDS             48018..48758
                     /gene="yggE"
                     /locus_tag="ACHKG5_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0866370.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            48925..49821
                     /gene="ygfI"
                     /locus_tag="ACHKG5_000180"
                     /pseudo
     CDS             48925..49821
                     /gene="ygfI"
                     /locus_tag="ACHKG5_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(49818..51296)
                     /gene="scpC"
                     /locus_tag="ACHKG5_000181"
     CDS             complement(49818..51296)
                     /gene="scpC"
                     /locus_tag="ACHKG5_000181"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0866371.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(51320..52105)
                     /gene="scpB"
                     /locus_tag="ACHKG5_000182"
     CDS             complement(51320..52105)
                     /gene="scpB"
                     /locus_tag="ACHKG5_000182"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0866372.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(52116..53111)
                     /gene="meaB"
                     /locus_tag="ACHKG5_000183"
     CDS             complement(52116..53111)
                     /gene="meaB"
                     /locus_tag="ACHKG5_000183"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0866373.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(53104..55248)
                     /gene="scpA"
                     /locus_tag="ACHKG5_000184"
     CDS             complement(53104..55248)
                     /gene="scpA"
                     /locus_tag="ACHKG5_000184"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP0866374.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(55452..56345)
                     /gene="argP"
                     /locus_tag="ACHKG5_000185"
     CDS             complement(55452..56345)
                     /gene="argP"
                     /locus_tag="ACHKG5_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0866375.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            56728..57387
                     /gene="rpiA"
                     /locus_tag="ACHKG5_000186"
     CDS             56728..57387
                     /gene="rpiA"
                     /locus_tag="ACHKG5_000186"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0866376.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            57642..58874
                     /gene="serA"
                     /locus_tag="ACHKG5_000187"
     CDS             57642..58874
                     /gene="serA"
                     /locus_tag="ACHKG5_000187"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0866377.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            59103..59162
                     /gene="ibsC"
                     /locus_tag="ACHKG5_000188"
     CDS             59103..59162
                     /gene="ibsC"
                     /locus_tag="ACHKG5_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0866378.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            59496..59555
                     /locus_tag="ACHKG5_000189"
     CDS             59496..59555
                     /locus_tag="ACHKG5_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0866379.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(59656..60258)
                     /gene="fau"
                     /locus_tag="ACHKG5_000190"
     CDS             complement(59656..60258)
                     /gene="fau"
                     /locus_tag="ACHKG5_000190"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0866380.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(60279..60462)
                     /gene="ssrS"
                     /locus_tag="ACHKG5_000191"
     ncRNA           complement(60279..60462)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKG5_000191"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(60504..60833)
                     /gene="zapA"
                     /locus_tag="ACHKG5_000192"
     CDS             complement(60504..60833)
                     /gene="zapA"
                     /locus_tag="ACHKG5_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0866381.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            61001..61579
                     /gene="ygfB"
                     /locus_tag="ACHKG5_000193"
     CDS             61001..61579
                     /gene="ygfB"
                     /locus_tag="ACHKG5_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0866382.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            61605..62930
                     /gene="pepP"
                     /locus_tag="ACHKG5_000194"
     CDS             61605..62930
                     /gene="pepP"
                     /locus_tag="ACHKG5_000194"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0866383.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            62927..64105
                     /gene="ubiH"
                     /locus_tag="ACHKG5_000195"
                     /gene_synonym="visB"
     CDS             62927..64105
                     /gene="ubiH"
                     /locus_tag="ACHKG5_000195"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0866384.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            64129..65331
                     /gene="ubiI"
                     /locus_tag="ACHKG5_000196"
                     /gene_synonym="visC"
     CDS             64129..65331
                     /gene="ubiI"
                     /locus_tag="ACHKG5_000196"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0866385.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(65405..65488)
                     /locus_tag="ACHKG5_000197"
     CDS             complement(65405..65488)
                     /locus_tag="ACHKG5_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866386.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            65779..66873
                     /gene="gcvT"
                     /locus_tag="ACHKG5_000198"
     CDS             65779..66873
                     /gene="gcvT"
                     /locus_tag="ACHKG5_000198"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0866387.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            66897..67286
                     /gene="gcvH"
                     /locus_tag="ACHKG5_000199"
     CDS             66897..67286
                     /gene="gcvH"
                     /locus_tag="ACHKG5_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0866388.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            67404..70277
                     /gene="gcvP"
                     /locus_tag="ACHKG5_000200"
     CDS             67404..70277
                     /gene="gcvP"
                     /locus_tag="ACHKG5_000200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0866389.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(70443..71876)
                     /gene="bglA"
                     /locus_tag="ACHKG5_000201"
     CDS             complement(70443..71876)
                     /gene="bglA"
                     /locus_tag="ACHKG5_000201"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0866390.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            71921..72232
                     /gene="yqfB"
                     /locus_tag="ACHKG5_000202"
     CDS             71921..72232
                     /gene="yqfB"
                     /locus_tag="ACHKG5_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0866391.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            72396..73055
                     /gene="yqfA"
                     /locus_tag="ACHKG5_000203"
     CDS             72396..73055
                     /gene="yqfA"
                     /locus_tag="ACHKG5_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0866392.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(73132..74112)
                     /gene="ygfZ"
                     /locus_tag="ACHKG5_000204"
     CDS             complement(73132..74112)
                     /gene="ygfZ"
                     /locus_tag="ACHKG5_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0866393.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            74355..74621
                     /gene="sdhE"
                     /locus_tag="ACHKG5_000205"
     CDS             74355..74621
                     /gene="sdhE"
                     /locus_tag="ACHKG5_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0866394.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            74602..75009
                     /gene="ygfX"
                     /locus_tag="ACHKG5_000206"
     CDS             74602..75009
                     /gene="ygfX"
                     /locus_tag="ACHKG5_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0866395.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(75049..75570)
                     /gene="fldB"
                     /locus_tag="ACHKG5_000207"
     CDS             complement(75049..75570)
                     /gene="fldB"
                     /locus_tag="ACHKG5_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0866396.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            75682..76578
                     /gene="xerD"
                     /locus_tag="ACHKG5_000208"
     CDS             75682..76578
                     /gene="xerD"
                     /locus_tag="ACHKG5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0866397.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            76603..77313
                     /gene="dsbC"
                     /locus_tag="ACHKG5_000209"
     CDS             76603..77313
                     /gene="dsbC"
                     /locus_tag="ACHKG5_000209"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0866398.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            77319..79052
                     /gene="recJ"
                     /locus_tag="ACHKG5_000210"
     CDS             77319..79052
                     /gene="recJ"
                     /locus_tag="ACHKG5_000210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0866399.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            79143..80241
                     /gene="prfB"
                     /locus_tag="ACHKG5_000211"
     CDS             join(79143..79217,79219..80241)
                     /gene="prfB"
                     /locus_tag="ACHKG5_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0866400.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            80251..81768
                     /gene="lysS"
                     /locus_tag="ACHKG5_000212"
     CDS             80251..81768
                     /gene="lysS"
                     /locus_tag="ACHKG5_000212"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0866401.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(81811..82359)
                     /gene="idi"
                     /locus_tag="ACHKG5_000213"
     CDS             complement(81811..82359)
                     /gene="idi"
                     /locus_tag="ACHKG5_000213"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0866402.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            82414..82485
                     /locus_tag="ACHKG5_000214"
     CDS             82414..82485
                     /locus_tag="ACHKG5_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866403.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(82482..82607)
                     /gene="yqfG"
                     /locus_tag="ACHKG5_000215"
     CDS             complement(82482..82607)
                     /gene="yqfG"
                     /locus_tag="ACHKG5_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0866404.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(82609..84057)
                     /gene="uacT"
                     /locus_tag="ACHKG5_000216"
     CDS             complement(82609..84057)
                     /gene="uacT"
                     /locus_tag="ACHKG5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0866405.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            84493..86412
                     /gene="ygfT"
                     /locus_tag="ACHKG5_000217"
     CDS             84493..86412
                     /gene="ygfT"
                     /locus_tag="ACHKG5_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0866406.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            86412..86900
                     /gene="ygfS"
                     /locus_tag="ACHKG5_000218"
                     /pseudo
     CDS             86412..86900
                     /gene="ygfS"
                     /locus_tag="ACHKG5_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(86936..88303)
                     /gene="ghxQ"
                     /locus_tag="ACHKG5_000219"
                     /gene_synonym="ygfQ"
     CDS             complement(86936..88303)
                     /gene="ghxQ"
                     /locus_tag="ACHKG5_000219"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0866407.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(88339..89655)
                     /gene="guaD"
                     /locus_tag="ACHKG5_000220"
     CDS             complement(88339..89655)
                     /gene="guaD"
                     /locus_tag="ACHKG5_000220"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0866408.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(89673..91073)
                     /gene="xanQ"
                     /locus_tag="ACHKG5_000221"
     CDS             complement(89673..91073)
                     /gene="xanQ"
                     /locus_tag="ACHKG5_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0866409.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(91238..94108)
                     /gene="xdhD"
                     /locus_tag="ACHKG5_000222"
     CDS             complement(91238..94108)
                     /gene="xdhD"
                     /locus_tag="ACHKG5_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0866410.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(94105..94884)
                     /gene="ygfM"
                     /locus_tag="ACHKG5_000223"
     CDS             complement(94105..94884)
                     /gene="ygfM"
                     /locus_tag="ACHKG5_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0866411.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(94934..96261)
                     /gene="ssnA"
                     /locus_tag="ACHKG5_000224"
                     /pseudo
     CDS             complement(94934..96261)
                     /gene="ssnA"
                     /locus_tag="ACHKG5_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
     gene            complement(96264..99362)
                     /gene="ygfK"
                     /locus_tag="ACHKG5_000225"
     CDS             complement(96264..99362)
                     /gene="ygfK"
                     /locus_tag="ACHKG5_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0866412.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(99684..100262)
                     /gene="mocA"
                     /locus_tag="ACHKG5_000226"
     CDS             complement(99684..100262)
                     /gene="mocA"
                     /locus_tag="ACHKG5_000226"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0866413.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            100365..101135
                     /gene="yqeC"
                     /locus_tag="ACHKG5_000227"
     CDS             100365..101135
                     /gene="yqeC"
                     /locus_tag="ACHKG5_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0866414.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            101183..102808
                     /gene="yqeB"
                     /locus_tag="ACHKG5_000228"
     CDS             101183..102808
                     /gene="yqeB"
                     /locus_tag="ACHKG5_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0866415.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(102849..103781)
                     /gene="arcC"
                     /locus_tag="ACHKG5_000229"
     CDS             complement(102849..103781)
                     /gene="arcC"
                     /locus_tag="ACHKG5_000229"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0866416.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(103829..105214)
                     /gene="hyuA"
                     /locus_tag="ACHKG5_000230"
     CDS             complement(103829..105214)
                     /gene="hyuA"
                     /locus_tag="ACHKG5_000230"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0866417.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(105267..106478)
                     /gene="ygeY"
                     /locus_tag="ACHKG5_000231"
     CDS             complement(105267..106478)
                     /gene="ygeY"
                     /locus_tag="ACHKG5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0866418.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(106536..107732)
                     /gene="dpaL"
                     /locus_tag="ACHKG5_000232"
     CDS             complement(106536..107732)
                     /gene="dpaL"
                     /locus_tag="ACHKG5_000232"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0866419.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(107790..108977)
                     /gene="ygeW"
                     /locus_tag="ACHKG5_000233"
     CDS             complement(107790..108977)
                     /gene="ygeW"
                     /locus_tag="ACHKG5_000233"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0866420.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            109456..111234
                     /gene="ygeV"
                     /locus_tag="ACHKG5_000234"
     CDS             109456..111234
                     /gene="ygeV"
                     /locus_tag="ACHKG5_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0866421.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(111274..111753)
                     /gene="xdhC"
                     /locus_tag="ACHKG5_000235"
     CDS             complement(111274..111753)
                     /gene="xdhC"
                     /locus_tag="ACHKG5_000235"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0866422.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(111750..112628)
                     /gene="xdhB"
                     /locus_tag="ACHKG5_000236"
     CDS             complement(111750..112628)
                     /gene="xdhB"
                     /locus_tag="ACHKG5_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0866423.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(112639..114936)
                     /gene="xdhA"
                     /locus_tag="ACHKG5_000237"
     CDS             complement(112639..114936)
                     /gene="xdhA"
                     /locus_tag="ACHKG5_000237"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0866424.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            115352..116107
                     /gene="actS"
                     /locus_tag="ACHKG5_000238"
     CDS             115352..116107
                     /gene="actS"
                     /locus_tag="ACHKG5_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0866425.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTILVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            116186..116259
                     /locus_tag="ACHKG5_000239"
     tRNA            116186..116259
                     /locus_tag="ACHKG5_000239"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:116218..116220,aa:Gly,seq:ccc)
     gene            116493..118098
                     /locus_tag="ACHKG5_000240"
                     /pseudo
     CDS             116493..118098
                     /locus_tag="ACHKG5_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            118165..118344
                     /locus_tag="ACHKG5_000241"
     CDS             118165..118344
                     /locus_tag="ACHKG5_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFP0866426.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        28945 a        29436 c        31179 g        29021 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacgt gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgtagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacccg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcaaaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    23881 cgcttacact aacatttatc ttagttatga tttaagtgca tgaaaataat tgagttatat
    23941 catcatagat taaacgctgt tatctgcaat taagacttta ctgaaaagaa atgtaacaac
    24001 tgtgaaaacc gaataataat atggaaatat tacaaatctt tgtctgttgt ggttggtgta
    24061 atcggtttca gttgtgatga taatttgcgg tgaaacgtga tcaggaagaa tgatgttatt
    24121 gtgcaggcgg tttttttgcc ggatgcggcg tgaacgccta tccaccgcgg tgaggtaaaa
    24181 gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga agaaagatta cttcagaccg
    24241 gcagcatcgc gcagcagctg cgctttgtcg gttttttccc acgggaaatg ttcacgacca
    24301 aagtgaccgt atgctgcggt ttctttgtag atcgggtgca gcagatccag catctgaatc
    24361 agaccgtatg ggcgcaggtc gaagaactca cgtaccagca gagtcagttg ttcagaaggc
    24421 actttctcag taccgaaagt ttctaccatg atggaagtcg gttcagccac gccgattgcg
    24481 taggaaacct gaatttcaca acgatcggcc aggccagcag caacgatgtt tttcgcgaca
    24541 taacgtgctg cgtaggctgc ggaacggtcc acttttgatg gatctttacc agagaatgca
    24601 ccgccaccgt gacgcgccat gccgccgtag gtatcaacga taattttacg accagtcaga
    24661 ccgcagtcac ccatcgggcc accgataaca aaacgaccgg tcgggttgat gaagaatttg
    24721 gtggcagaag tcagccattc agcgggcaga atcggcttga tgatctcttc cattaccgct
    24781 tcttgcagcg atttctggtc gatctcttca gagtgctgag tggaaagcac gacagcatcg
    24841 ataccaacga ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc gtccgggcgc
    24901 agccacggca gagtgccgtt tttacgcact tcagcctgac gctgtaccag acggtgtgca
    24961 taggtgatag gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc aaacatcaga
    25021 ccctggtcac ccgcgccctg ttccagcgga tcggcacggt caacgccctg gttgatgtca
    25081 ggagactgtt tgccgatagc gctcagaact gcacaggagt tagcgtcaaa gcccatgtcg
    25141 gaatgcacat agccaatttc gcgaacggtg ttacgggtga tctcttcgat gtctacccag
    25201 gcgctggtgg tgatttcgcc gccaactaaa accatgccgg tttttacgta ggtttcgcaa
    25261 gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc agagatttgg
    25321 tcagcaattt tgtcaggatg cccttcagag acggactcgg acgtaaaaag gtgttttgcc
    25381 atatttaata tcacctaaag agaatttggt tagctcaaac tgttgtgtgg attttctgtg
    25441 gtagcggatc ctaccacgac tctgcaggtt aaaaacactg gcagtctgag tgttaatcgg
    25501 tatggatgga ttaacatctg gatggctatt ttaggtcaat tcttcacccg atttccactt
    25561 ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc
    25621 ggcattctgc gtatttatct tttgcaattt tctgccattg tggggtataa aacgcggcgc
    25681 gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg ccacttccag ccgggttgaa
    25741 atcagagttt tggcttgtgg gttcgtctta acaggcggcc gtggaggtga tacgaaataa
    25801 tgaaccgttg tctgctgctt aacctgtctc accgttctgg tgaagattcg ttccccgcac
    25861 tctgcatctc tgctttgcat acctgccgat gttataccca tctcggcgct tctcaggatt
    25921 caagagctgg ttacagttac tgaggactga acaagggcgc tcttgtaaaa acaagagttt
    25981 tctcgtggtt tcgccgaact gtcacactta cgttcggtta tgtgcttaat aatgttatga
    26041 aaaagaaacc ggttgcgcag ttggagcgtc agcattcact gctggaaaat ccatgtgctt
    26101 atgggttgtt atcgcagttc caggctgcga tagtcgttaa ctgttttaca cttaataaaa
    26161 taatttgagg ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt cagcgggcga
    26221 acacggtgta ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat
    26281 gctgcgtact tacaatattg cctggtgggg caataactac tatgacgtta acgagctggg
    26341 ccacatcagc gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt
    26401 agtgaaaact cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt tcccacagat
    26461 cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg
    26521 ctataacggc gattacttcc ttgtttatcc gatcaaagtt aaccagcacc gtcgcgtgat
    26581 tgagtccctg attcattcgg gcgaaccgct gggtctggaa gccggttcca aagccgagtt
    26641 gatggcagtg ctggcacatg ctggcatgac ccgtagcgtc atcgtctgca atggttataa
    26701 agaccgcgaa tatatccgcc tggcattaat tggcgagaag atggggcaca aggtctatct
    26761 ggtcattgag aagatgtcag aaatcgccat tgtgctggat gaagcagaac gtctgaatgt
    26821 cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag ggctccggta aatggcagtc
    26881 ctccggcggg gaaaaatcga agttcggcct ggcggcgact caggtactgc aactggttga
    26941 aaccctacgt gaagccgggc gtctcgacag cctgcaacta ctgcacttcc acctcggttc
    27001 gcagatggcg aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt
    27061 tgagctgcac aagctgggcg tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt
    27121 ggattatgaa ggtactcgtt cgcagtccga ctgttcggtg aactacggcc tcaatgaata
    27181 tgccaacaac atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc
    27241 gacggtaatc accgaatcgg gtcgtgcagt gactgcgcat cacaccgtgc tggtgtctaa
    27301 tatcatcggc gtggaacgta acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc
    27361 gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg cacgaaccgg gaactcgccg
    27421 ttctctgcgt gaatggttac acgacagtca gatggatctg cacgacattc atatcggcta
    27481 ctcttccggc acctttagcc tgcaagaacg tgcatgggct gagcagcttt atttgagcat
    27541 gtgccatgaa gtgcaaaagc agctggatcc gcaaaaccgt gctcatcgtc cgattatcga
    27601 cgagctgcag gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat
    27661 gccggatgcg tgggggatcg accagttgtt cccggttctg ccgctggaag ggctggatca
    27721 agtgccggaa cgccgcgctg tgctgctgga tattacctgt gactctgacg gtgctatcga
    27781 ccactatatc gatggtgatg gtattgccac gacaatgcct atgccggagt acgatccaga
    27841 gaatccgccg atgctcggtt tctttatggt cggcgcatat caggagatcc tcggcaacat
    27901 gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc gtcttccctg acggtagtgt
    27961 agaagtggaa ctgtctgacg aaggcgatac cgtggcggac atgctgcaat atgtacagct
    28021 cgatccgaaa acgctgttaa ctcagttccg tgatcaagtg aagaaaaccg atcttgatgc
    28081 tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt atacctatct
    28141 tgaagatgaa taattaagat tattctgctg gcgggtcgat ttatttacgt aaatcggtcc
    28201 gctttctttt tatatattct taaaaataat ttagctgctt ttcttattaa ataatctccc
    28261 tacaatcgct gcattaatta aatgcagagg gaacggttat gaaaaaatgg aaagtacgca
    28321 gcgcgttggt ggcattaatc gtgctgctgg ccggttgttc cagtaatgcg cagtataata
    28381 gcagtgcatc gggcaatgtc ggcaccgcct ggggtgggga tgtgcactca acggttcagg
    28441 gcgtatctgc tgagcgagcc tggcgcgatc cggcggagat gatcgttatt agttattcga
    28501 ctaatgttcc ttccggctac gatcgcgttt atagcattcg tattaacgaa ctggaatatg
    28561 ctattcgcga tggtaatttt aattctctac ctattacgcg tgtttatgat tcatctaata
    28621 atgaaccacg ctatattgtc catgccagag tcggaatgaa ttatcaatta tatgtccgta
    28681 attacagtcg taacaccaac tacgagatcg ttgctacggt tgatggcctg gatgtgctga
    28741 acggcaagca aggttcgctg aataacaacg gctatattgt gaatgccgga gattcgttag
    28801 cgattaaagg attccgcaaa gataaacata ccgaagcggc ttttcagttc gccaacgttg
    28861 ctgattccta cgcagccaat tcggcgcaag gcgatgtgcg taataccggt gtaatcggct
    28921 ttgccgcctt tgaattacaa ggccaggcgc aaaacgcgct gccaccgtgc tctggacaag
    28981 cctttccggc agataacaat ggctatgcgc cgccaccgtg tcggaaatag caacctcgct
    29041 tgatgcctcg cccatttaag gcgataatta gcacaaactt gtgtttcgca aatccaaccc
    29101 ttcctcgtcg ggcctaacga cgcggaaggg tttttttata tcgactttgt aataggagtc
    29161 catccatgag caccttaggt catcaatacg ataactcact ggtttccaac gcctttggtt
    29221 ttttgcgcct gccgatgaac ttccagccgt atgacagcga tgctgactgg gtgattactg
    29281 gtgtgccgtt cgatatggcc acttctggtc gtgcgggggg acgccacggt ccggcagcta
    29341 tccgtcaggt ttcgacgaat ctggcctggg aacacaaccg cttcccgtgg aatttcgaca
    29401 tgcgtgagcg tctgaacgtc gtggactgtg gtgatctggt atatgctttc ggcgatgccc
    29461 gtgagatgag cgagaagctg caggcgcacg ccgagaagct gctggctgcc ggtaagcgta
    29521 tgctctcctt cggtggtgac cactttgtta cgctgccgct gctgcgtgct catgcgaagc
    29581 atttcggcaa aatggcgctg gtacactttg acgcccacac cgatacctac gcgaacggtt
    29641 gtgaatttga ccacggcacc atgttctata ccgcgccgaa agaaggtctg atcgacccga
    29701 atcattccgt gcagattggt attcgtaccg aatttgataa agacaacggc tttaccgtgc
    29761 tggacgcctg ccaggtgaac gatcgcagcg tggatgacgt tatcgcccag gtgaaacaaa
    29821 ttgtgggtga tatgccggtt tacctgacct ttgatatcga ctgcctggat cctgcatttg
    29881 cgccgggaac cggtacgccg gtaattggcg gtctgacctc cgatcgcgct attaaactgg
    29941 tacgcggcct gaaagatctc aacatcgttg ggatggacgt agtggaagtg gctccggcat
    30001 acgatcagtc ggaaatcacc gctctggcag cggcaacgct ggcgctggaa atgctgtata
    30061 ttcaggcggc gaaaaagggc gagtaagcac cagatgcgat gcgcacgggt aaaacgtgcc
    30121 attaatgtcg gatgcggcgt gaacgcctta tccgacctac gttcggcacc cgtaggccgg
    30181 ataagatgcg ccagcatcgc atccggcaat gcgcacaagg taacaaatgt gccattcatg
    30241 tcagatgcgg cgtgaacgcc ttatctgacc tacgttcgac accaccaggc tttacttaat
    30301 cccatccgcg ctcatgcgat cgcgaatatg ctgggcgcgt tcggcggatg caggatggtc
    30361 gtcaaacatt gagctttggc gaccttcttc cagttttgcc agtttttcaa agctggtggc
    30421 aagacctgcc gggctgatgc cgcgttggcg cagaagatcg taagaataat catcggcttc
    30481 tgcttcctgg cgctgggaga attgcgaatt aactaatttc tcgcccagat caccgagttg
    30541 tgattgagat aaactgccga caatcccgcc cgccgaggcc gcagctactc gtacggcatt
    30601 tgtaccaagt gccacctgca ttcctttttt cacatggcct aatgccacgt gccccatttc
    30661 gtgaccgatc accgcttcga cttcgttatc cgtcatcata tccatcagcc cgctatagac
    30721 acggatacag ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat acactttgta
    30781 atttaccggc tgaccgttga tattgttgcc tagcgcattg gcaatagttg tcagacgttt
    30841 agcgtattcg ctattggctg gcgcaatcgt cgccttgctg tccatctcct gacatgcctg
    30901 atcgctcagg gttttcacct gcgcatcact caaactgtaa gcctgaaaag cttccgctcc
    30961 tgatgagagc agtccgttgg agtccatatt ctggcaaccg gtcagtaccg ttgccacgct
    31021 cattgctacc aataaggcgc gaattttcat gttttttctt ccgcacaagg tcaggattat
    31081 tctaaaaatg cgccgtttgc aggtgaaccg acgctcagtc tcagtataag gaaaagcgca
    31141 gcggacgggc gagtagattg cgcaacatgc gagcatgatc cagagatttc tgaagcggca
    31201 aaaggatgtt ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa attttccggc
    31261 gtagcccaaa acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca tccgatctgg
    31321 agtcaaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga gcatggacgc
    31381 agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg acattgccga
    31441 agtcctgtgg cgtgatttcc tgaaacataa cccgcagaat ccgtcctggg ctgaccgtga
    31501 ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc tgcacctcac
    31561 cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact ctaaaactcc
    31621 gggccacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc cgctggggca
    31681 gggtattgcc aacgcagtag gtatggcgat tgcagaaaaa acgctggcgg cgcagtttaa
    31741 ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg acggctgcat
    31801 gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc tgggtaaact
    31861 gattgcgttc tacgatgaca acggtatttc tatcgatggt cacgttgaag gctggttcac
    31921 cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg acatcgacgg
    31981 tcatgacgca gcatccatca aacgcgcagt agaagaagcg cgcgcagtga ctgacaaacc
    32041 gtccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag ccggtactca
    32101 cgactcccac ggtgcgccgc tgggtgatgc agaaatcgct ctgacccgcg aacagctggg
    32161 ctggaaatac gcaccgttcg aaatcccgtc tgaaatctat gcgcagtggg atgcgaaaga
    32221 agcaggccag gcgaaagaat ctgcatggaa tgagaagttt gcggcttacg cgaaagctta
    32281 tccgcaggaa gcggctgaat ttacccgccg tatgaaaggc gaaatgccgt ctgacttcga
    32341 cgccaaagcg aaagagttta tcgctaaact gcaggctaat ccggcgaaaa tcgccagccg
    32401 taaagcgtct cagaatgcta tcgaagcgtt cggcccgctg ttgccggaat tcctcggcgg
    32461 ctccgctgac ctggcaccgt ctaacctgac cctgtggtct ggttctaaag caatcaacga
    32521 agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga ccgcgattgc
    32581 taacggtatc tccctgcacg gtggcttcct gccgtacacc tccaccttcc tgatgtttgt
    32641 cgaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc aggttatggt
    32701 ttacacccac gactccatcg gtctgggcga agatggcccg actcaccagc cggttgagca
    32761 ggttgcttct ctgcgcgtga ccccgaacat gtctacatgg cgtccgtgtg accaggttga
    32821 atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggtccgaccg cgcttatcct
    32881 ctcccgtcag aacctggcgc agcaggaacg tactgaagag caactggcaa acatcgcgcg
    32941 cggtggttat gtgctgaaag actgtgctgg tcagccggaa ctgatcttca tcgcgaccgg
    33001 ttcggaagtt gaactggctg ttgccgccta cgaaaaactg actgccgaag gcgtgaaggc
    33061 gcgcgtggtt tccatgccgt ctaccgatgc attcgacaag caggatgcgg cttaccgtga
    33121 atccgtactg ccgaaagcgg ttactgcacg cgttgcggta gaagcgggta ttgctgacta
    33181 ctggtacaag tatgttggcc tgaatggcgc tatcatcggt atgaccacct tcggtgagtc
    33241 agctccggca gagttgctgt tcgaagagtt cggcttcacc gtcgacaacg tcgttgcgaa
    33301 agcaaaagaa ctgctgtaac tagcatttcg ggtaaaaagg tcgcttcggc gacctttttt
    33361 attaccttga tatgtccgtt tgcggacaag caatagataa agcgtgttgt agatcgcaaa
    33421 tatttatatg caataaatat caattatgta atatgcatca cgatatgcgt gttgacattt
    33481 gttgttatat ctataactca atgttatata agaaattaat aattcactgt tttcaaaaca
    33541 ccggtttccc tgctcaattg ctttcattaa accgcacgag tattttattt ttaccgccgg
    33601 acggtaccct aaaaataaaa taaggataat ttatgcggct tagtgactat tttccagaat
    33661 catcaatctc agttatacat tcagcaaaag attggcagga agctatcgat ttctcgatgg
    33721 tatcattgct ggataaaaac tatatcagcg agaattacat tcaggcaatt aaagattcca
    33781 ccattaacaa tggcccttat tatattctcg caccaggcgt ggcaatgcct catgcgcgac
    33841 cggaatgtgg ggcgcttaaa accgggatgt ctttgacatt acttgaacaa ggtgtttatt
    33901 ttccggggaa tgacgaacca attaaattac tcatcggact ctctgctgcc gatgccgatt
    33961 cgcacattgg cgctattcag gcgttaagtg aattactgtg cgaagaagaa atactcgaac
    34021 aactcttaac agcatcatca gaaaaacaat tagcggacat tatcagccgc ggataattac
    34081 tctctcctct acaggataat attatggaaa acaagtctgc tcgtgcaaag gtccaggctt
    34141 ttgggggctt tttgactgca atggtcatcc ccaatattgg tgcttttatt gcctggggtt
    34201 ttattactgc gttatttatt cccaccggtt ggctgcctaa cgaacatttc gccaaaattg
    34261 tcggcccgat gattacctat ttattgcccg tgatgattgg ttctaccggt ggtcatctgg
    34321 tcggcggtaa acgcggcgcg gtcatgggcg gaataggtac tattggtgtg atcgtcggcg
    34381 cagagatccc gatgttcctt ggctcaatga ttatggggcc gctcggtggt ctggtcataa
    34441 aatatgttga taaggcactg gaaaaacgca tacctgccgg ttttgagatg gttatcaata
    34501 acttctcatt aggtatcgcg gggatgctcc tttgtctgct gggttttgaa gtgatcggcc
    34561 cggcggtgtt aattgccaat actttcgtca aagagtgtat tgaggcgctg gtacatgcgg
    34621 gttatctgcc tctgctgtca gtcatcaatg aaccggcgaa agtgcttttc cttaataatg
    34681 cgatcgatca gggcgtctat tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat
    34741 ccatcttctt tatggtggcc tctaacccag gtccgggcct ggggctgctg ctggcgttta
    34801 ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc
    34861 tcggtgggat ccacgaactg tatttcccat atgtgctgat gaagccgctg actattattg
    34921 ccatgattgc gggcggtatg tctggcacct ggatgtttaa cttactggac ggtggtctgg
    34981 tggctggccc aagtccgggg tctatctttg cttacctggc gctgacgccg aaaggttcgt
    35041 tcctggcgac aattgccggt gttacggtag gtaccctggt gtcctttgct atcacttcgc
    35101 tgatactgaa gatggaaaaa acggtggaaa cggagagcga agatgagttt gctcagtcag
    35161 ccaatgcggt taaggcgatg aaacaagagg gtgcattctc gttaagcagg gttaagcgta
    35221 tcgcctttgt ttgcgatgcg gggatgggct ccagtgcgat gggcgcgacc accttccgta
    35281 aacgcctgga aaaagcgggg ctggcaattg aagtaaaaca ttacgccata gaaaacgtgc
    35341 ctgcggatgc ggatatcgtc gttactcatg ccagtctgga agggcgcgtg aaacgtgtga
    35401 cggataaacc actgatattg attaataact atattggcga tccaaaactc gacactttat
    35461 ttaatcaatt aaccgccgaa cataaacact gattacagag gtaaaaatga aaaccaaagt
    35521 tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa tttgaactgc cagaaattac
    35581 cgataatgaa ttactggtga gtgtgatttc tgacagcgtc tgtttatcca cctggaaagc
    35641 ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc atccggtcat
    35701 taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca ctggcaaata
    35761 taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg gatattcagc
    35821 gggctacagc tacgaatatt ttggcggcaa tgccacttat atgattattc ccgaaatcgc
    35881 cattaatttg ggctgcgtat taccgtatca cggctcttat tttgctgcgg cgtcgctggc
    35941 agagcctatg tgctgcatta ttggcgctta tcatgccaat tatcacacca cgcaatatgt
    36001 ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt gcactgctgg cgtgtgcagg
    36061 tccgatgggc attggcgcta tcgattacgc cattaacggc ggcatacaac cgtcgcgggt
    36121 ggtggtggtc gatatcgacg acaaacgtct ggcgcaggta cagaagctgc tgccggtgga
    36181 actggcggcc agtaaaggca ttgagctggt gtatgtgaat accaaaggga tgagcgatcc
    36241 tgtccagatg ctgcgggcgc tgacaggaga tgccgggttc gatgacattt ttgtttatgc
    36301 ggcggtgtct gctgtcgttg agatggctga tgaattactg gcggaagatg gctgtctgaa
    36361 cttctttgcc gggccgacgg ataaaaactt caaagtgccg tttaatttct acaacgttca
    36421 ttacaacagc acgcacgtcg tcggtacatc cggcggttca acggacgaca tgaaagaggc
    36481 gattgccctt agcgccacag ggcagttaca gccctcgttt atggtgaccc atatcggtgg
    36541 cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat atccctggcg gtaaaaaact
    36601 catttataac ggcgtgacca tgccgctcac tgccattgcc gattttgccg aaaaaggcaa
    36661 aaccgatccg ctgtttaaag agttggcgcg gctggttgag gaaacgcacg gcatctggaa
    36721 tgaacaggcc gagaaatatc tgctggcaca atttggcgtt gatatcgggg aggccgcgca
    36781 atgatgtccc tggcgtggcc attatttcgc gttacggaac aggcagcgtt ggctgcctgg
    36841 ccgcaaaccg gatgtggcga caaaaataaa attgatggcc tggcggtcac cgcgatgcgc
    36901 caggcattaa acgacgtcgc ttttcgtgga cgagtggtta tcggcgaagg agaaattgac
    36961 catgcgccga tgctgtggat tggcgaagag gtgggcaaag gcgatgggcc agaagtcgat
    37021 atcgcggttg atcccattga aggcacgcgg atggtggcga tggggcagag caatgcgctg
    37081 gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg cgcccgatat gtatatgaaa
    37141 aagctggttg ttaatcgact ggctgccggg gcgattgatc tgtctttgcc tctggcggac
    37201 aacttgcgtg acgtggcgcg ggcgttaggt aaaccgctgg acaagctgcg catggttacg
    37261 ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa cacagctggg cgtgaaggtt
    37321 tttgccctgc cggatggcga tgtcgccgcc agcgtgctga cctgctggca ggataatcct
    37381 tacgatgtga tgtacaccat cggcggtgcg ccggaaggcg tgatttctgc ctgtgccgtt
    37441 aaagcattag gcgggaatat gcaggccgag ctgattgact tttgtcaggc caaaggggat
    37501 tacacggaaa atcggcagat tgcagagcag gagcgcaagc gttgcaaggc aatgggtgtt
    37561 gacgttaacc gtgtgtactc gctcgatgta ctggtaaggg ggaacgatat cctctttagc
    37621 gctacgggcg tcacgggcgg cgagttggtg aacggtatcc aacagacggc gaatggagtg
    37681 cggacgcaga cattactgat cggcggcgcg gaccaaacgt gtaatataat agactccctg
    37741 tattgatggt gatctatcgg aagaccaatg gcgacgctgt cagaagatga tgtgcttgag
    37801 caactggatg cacaggacaa tttattttca tttatgaaaa ctgcgcattc tattttgctc
    37861 caggggatac gccagtttct gccgtcgctg tttgtcgata acgatgaaga gagcgtcgaa
    37921 tatgcagtga agccgttact cgcccaaagc ggcccgcttg acgatattga tgttgcactg
    37981 cgtttgattt atgcgctggg gaaaatggat aaatggctgt acgccgatat cacgcatttt
    38041 tcccagtact ggcattacct gaaagaacag gatgaaacgc tcggatttgc cgatgacatg
    38101 acttgggatt ttatcagcaa cgtcaatagc ataacctgca atgcgacgct ctatgacgcg
    38161 ttgaaagcga tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt tagcggaatg
    38221 gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa aggaattaac gccgtgaaaa
    38281 ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta ccatgatgaa gagatagaac
    38341 gcgtgcataa accgttgctg cgtatgctgg cggccttaca gacggtaaat ccgcagcgcc
    38401 gaaccgtggt ttttctttgc gctccgccgg ggacgggtaa atctacccta accaccttct
    38461 gggcatatct cgcgcaacaa gacccggagc tgcccgctat tcagacgctc ccgatggatg
    38521 gttttcacca ttacaatagc tggctggatg cgcatcaatt gcgccccttc aaaggcgcac
    38581 cagagacatt tgacgttgcg aaactggcgg aaaatctgcg ccaggtcgtg gaaggggatt
    38641 gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta
    38701 ccgcaccaat aatcatcgtc gaaggaaact ggctattgct ggatgacgat aactggcgcc
    38761 agctagcgca attttgtgat ttctcgatct ttatcaatgc gccagctact gcgttgcggg
    38821 aacgactggt ggggcgcaag ctggcgggag ggttatcgct ggcagacgca gaagcttttt
    38881 atgaccgtac cgacgggccg aatgttcgcc gggtgctgga agagagtctg cctgcaaatt
    38941 tgaccttaat gatgaccgct acgggtgaat atcgtctggt ggattaatgt tcctgattag
    39001 accatcttcc agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact
    39061 gattgctacg acgctcgtgc ctcgctggcg gcacttctcc agccagcttt caaacacgct
    39121 caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc agcaatagtg gcggagagag
    39181 tgcttccagg caggcgacgg cgaccatccg tcgctgcgca ctatgcagat ccaacgggtg
    39241 cgcgctggca gtgtctgcta actgacaaca ttgcagggca gcatgggtac gttgagtgat
    39301 ttcatccgcc gggcattttt gcagtttcaa accaaatgcg atctcgtcag cgacggtgct
    39361 gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca atctttgccg cgcgctggcg
    39421 gtttttcagg tttttcatcg cctgttgttg cagcataacc gtaccggctg tgggcgtaag
    39481 aagacctgcc attactcgta acagcgtcga tttccctgcg ccattgtcgc cggtcaacgc
    39541 caaccattca ccttgtttga gttgcagaga aatgtcgcaa agacaatccg ttgccgcgcc
    39601 aggccagcga taagatatct gatttaacgt taacatagtg gctggagcgc tccgttttgc
    39661 aattgccacg cttgctggca gtagttcagg aaaggtgtgc gatgacgttc aaaaagaata
    39721 atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct gcaacagcat ttcgctggcc
    39781 tgcggcgtca ggcggctaaa ggcttcatca agaattaata gctttggctg catcgcgatg
    39841 gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga gtgttgccgg gtgacgatgg
    39901 cgcaacggct ggcactcggt cagagtcagg gcggcatcaa tccgcgccat aatctctgct
    39961 tcagcaagac acagattttc tggcccaaag gcaacttctt cttccacgct aaaggtacag
    40021 ccggagagct gcaaataggg cgattgctgg acgagctgga tggtggcgga ttgttcatta
    40081 agcgggagat gcccaatggg cgtaccgagg agcgtccccg tgcctgttat ctctcctggc
    40141 agaaagtccg gataccagcc cgccatcagc tgtgccagcg tacttttgcc actgccgttg
    40201 tcaccaaaga tggcgaccat ccccggtttg acatagtgaa agtcataaca gaacggagga
    40261 tgcgttgagt gtgtcgggca ataacggaac tgttctaacg taaccatatc cacgctccga
    40321 attctgtcag catcagcaag atcatggtgt aacgcgctac gcgttgtagg gtgctatcgg
    40381 ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa tgcccgcata tccagcgccg
    40441 caccacggac agcaagatcg tttagcgcat tgtgtgttag cggaatagtt aacgcgggca
    40501 tcgcacgtaa acgctgatac cagccctcat ccagcggcac gccgcgggca cgctgcgctt
    40561 catgaataat cgctaattgc cgttttaact gctcaacgac caataacggc ccggcaaaca
    40621 gataagcgac gcccggcggc aggcgagaag caaacagagc gcggataaaa cattgcacgg
    40681 ggacgtactg catccacaac tgtgaggttg aaacaatcgc cagaatccgc aaccagagcg
    40741 taatagcatg cgtccaacgc tccggcgaac gcggcgtgcc gcttagccat tctgttagcc
    40801 agccaccgtg taccagccac agcccggctc ccagcgagaa catcagccag acgacatatt
    40861 ttgcccgccg ccgcgtggcc tttagcgcaa taaggcaaaa aaaggtcgcc gcgctataga
    40921 tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca ggccgccaat gcccatagtg
    40981 ttaatgaggt aaatggatgc atttagcgca cggcagccat tgccggaaaa tggcgcgtgg
    41041 tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac cgctgtaaga gttttatcga
    41101 ccaggttcgc gcctatcacc gtaatcgcca ccgactccac caggttttgc cccatcgagt
    41161 gcatccaggc aacgaacaaa tcggctccgc taccggtgac accgccgaac agcgcagtac
    41221 gcagcggtac agcgacgacc gttaccgcaa gcgtaataat cacgccgctg acaacgactt
    41281 ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg ccgatgacca
    41341 tcgcgacggg ggcaaaagcg gcagcgatcg ggtcggtcag taatccccag agtaaattgg
    41401 tcagtaagcc ggtcagcatc ccgatcaccg gaccaagcag taccgcgcta ataagtgtgc
    41461 cgatggagtc gagaaatata ggtaatttga ccatgctggc cagttgcccg ccgatcatat
    41521 tgatggcgat agatataacg atcaacacca gtgcctgact ggaaaagtgg ctacgcgcca
    41581 taagaaaacc tctctgaaag tagttattta acgggggaaa tatgcaactc gtcgtaacca
    41641 gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa ggctaccgat caccagttca
    41701 agcgtggtcc gcacggtaca gccgggcatc atatgaccgc caagcatcgt gccgtgtgga
    41761 tcggaaacgc agagatgaag atgctcacca ctctgttcca acgtgccgtt tagtgagatc
    41821 acttcgaatg taccgttcag gagcgtggtg ccttcttgcc cggcatagcg caaggcgatg
    41881 tcagtcaggc taccggtaca gcctgcaatc caggcggcat gaagttgctg ctgctgtgca
    41941 aaagcgtgta gctgggaaaa aacttcctgc ccggggagga ggcgcagtgc gtaaaagcgt
    42001 gcggaggaag aatatgggta gggcaatgtc atcattggag tactccgaaa ctcggacgta
    42061 ttacggataa tatcgacgct gcatcagtca tattctgata taagtcaggg tttgtgacgg
    42121 cacaacgaaa agattttgct acgcttattc gtgattctgc aaagtggaat aaattccaca
    42181 ggggcggggc tgaaaacgtg acgtaagtca gataattgtt ttctttgact ggacaatcat
    42241 tccttttatt ccacgtttcg cttatcctag ctgaagcgtt tcagtcgatt aaatgttcga
    42301 caattaacca atcagtcgca gtttgcgaca ggtaaggttt ccccggacga tttgctggat
    42361 tactctgtct ggcaaatttg ctcagggaaa accttgcagg agatctatga ccgtacgcgt
    42421 agcgataaat ggcttcggtc gcatcgggcg taatgtggtt cgtgctttgt atgaatccgg
    42481 acgccgggcg gaaattaccg tggtggcaat caacgaactg gcggatgctg cgggcatggc
    42541 gcatttgttg aaatacgaca ccagccatgg ccgttttgca tgggaagtac gacaggaacg
    42601 cgatcaactt tttgttggtg atgacgccat ccgcgtattg catgaacgtt cactgcaatc
    42661 gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac tgcaccggcg tatatggctc
    42721 ccgcgagcat ggcgaagcgc acattgccgc cggggctaaa aaagtgctct tttcacatcc
    42781 tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc aatcaggatc aacttcgtgc
    42841 ggagcaccgc atcgtttcta acgcttcctg taccacgaat tgcataattc ccgtcatcaa
    42901 attgttagat gatgcgtacg gtattgagtc cggcactgtg accacaattc actccgccat
    42961 gcacgatcaa caggttattg atgcatacca tcctgacctg cgtcgcaccc gggcagccag
    43021 ccagtcgatc attccggtcg atactaaact ggccgccggt atcacacgat tttttccgca
    43081 atttaacgat cgctttgaag cgattgcggt acgtgtgcca accataaatg tgacggcaat
    43141 cgatttaagc gtgacggtga agaaacctgt aaaagccaat gaagtcaacc tgttgctgca
    43201 aaaagcagca caaggtgcat ttcatggtat agttgactat acggaattgc cgttggtctc
    43261 tgtagatttt aaccacgatc cgcacagtgc cattgtcgat ggcacccaaa cccgggtcag
    43321 tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac gaatggggct ttgctaaccg
    43381 aatgctcgac acgacgttag ctatggctac tgttgctttc aggtaagacg caagcagcgt
    43441 ctgcaaaact tttagaatca acgagaggat tcaccatgtc tgtaattaag atgaccgatc
    43501 tggatcttgc tgggaaacgt gtatttatcc gtgcggatct gaacgtacca gtaaaagacg
    43561 ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc gaccatcgaa ctggccctga
    43621 aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg tcctaccgaa ggcgagtaca
    43681 acgaagaatt ctctctgctg ccggttgtta actacctgaa agacaaactg tctaacccgg
    43741 ttcgtctggt taaagattac ctcgacggcg ttgacgttgc tgaaggtgaa ctggttgttc
    43801 tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga cgaaatcctg tccaaaaaat
    43861 acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg tactgctcac cgcgcgcagg
    43921 cttctactca cggtatcggt aaattcgctg acgttgcgtg cgcaggcccg ctgctggcag
    43981 ctgaactgga cgcgctgggt aaggcactga aagaacctgc tcgcccgatg gtggctatcg
    44041 ttggtggttc taaagtatct accaaactga ccgttctgga ctccctgtct aaaatcgctg
    44101 accagctgat tgttggtggt ggtatcgcta acacctttat cgcggcacaa ggccacgatg
    44161 tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc aaaacgtctg ctgaccacct
    44221 gcaacatccc ggttccgtct gatgttcgcg tagcaaccga gttctctgaa accgctccgg
    44281 ctaccctgaa atctgttaac gacgtgaaag ctgacgagca gatcctggat atcggtgatg
    44341 cttccgctca ggaactggct gaaatcctga agaatgcgaa aaccattctg tggaacggtc
    44401 cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac tgaaatcgtg gctaacgcta
    44461 tcgcagacag cgaagcgttc tccatcgctg gcggcggcga cactctggca gcaatcgacc
    44521 tgttcggcat tgctgacaaa atctcctaca tctccactgg cggcggcgca ttcctcgaat
    44581 tcgtggaagg taaagtactg cctgcagtag cgatgctcga agagcgcgct aagaagtaaa
    44641 aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc ttttagagca cgcatttttt
    44701 catactctaa ataattcgag ttgcaggaag gcgacaagag agtgaatccc caggagctta
    44761 cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac
    44821 gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga tttcgtaaaa
    44881 cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa agaaaacaac
    44941 ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt actggaaact
    45001 gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc ttcctttatc
    45061 gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg cgcgatctct
    45121 ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat cctgcacact
    45181 gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa
    45241 cacttcgcag ctaccggtaa gccgctgttc tcttctcaca tgatcgacct gtctgaagaa
    45301 tctctgcaag agaacatcga aatctgctct aaatacctgg agcgcatgtc caaaatcggc
    45361 atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt ggacaacagc
    45421 cacatggacg cttctgcact gtacacccag ccggaagacg ttgattacgc atacaccgaa
    45481 ctgagcaaaa tcagcccgcg tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt
    45541 tacaagccgg gtaacgtggt tctgactccg accatcctgc gtgattctca ggaatatgtt
    45601 tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg tggttccggt
    45661 tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat gaacatcgat
    45721 accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc gaacgaagct
    45781 tatctgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa gaaatactac
    45841 gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct ggaaaaagca
    45901 ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt atctcaaggc
    45961 ccgctctgcg ggtctttttt tcgccaaaag caaaattgcc tgatgcgcta tgcttatcag
    46021 gcctacacgt atcctgcaat atattgaatt tgcaggtttt tgtaggccgg ataaggcgtt
    46081 cacgccgcat ccggcatgac aaagcgcaca ttgatatctg tctgagcccc gctctgcggg
    46141 gatttttttg ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat ttggcaaaat
    46201 tatgctttat tgtttaccct tgtcagactg cccgtcataa ggcggcggag tgtatttctc
    46261 cattttgagt cagttgaaaa ggaatattga atggaagatt tgaatgttgt cgatagcata
    46321 aacggcgcgg gaagctggct ggtagctaac caggcgctgc tgctaagtta tgcagtaaac
    46381 atcgtggcgg cactcgcgat catcatcgtt ggtttgatta tcgcgcggat gatttccaac
    46441 gcggtgaatc gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga ttttctttct
    46501 gcattagtcc gttacggtat tatcgccttt acgctaatcg cagccctggg acgcgtgggt
    46561 gtacaaacag cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt tggtctggct
    46621 ttgcaggggt cactttctaa cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc
    46681 cgtgccggag aatatgttga cctcggcggc gtagccggta ctgtgctgag tgtgcagatt
    46741 ttctccacca ccatgcgtac tgcagacggt aaaattatcg ttattccgaa cggtaaaatt
    46801 attgccggaa atattattaa cttctcccgc gagccagttc gccgtaacga atttattatt
    46861 ggcgtggcgt atgattccga tatcgatcag gttaagcaga tcctgaccaa tattatccag
    46921 tctgaagatc gcattttgaa agatcgcgaa atgactgtgc gcctgaacga acttggtgca
    46981 tcgtcgatta atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac
    47041 tgggatgtgc tggagcgtat taaacgtgaa tttgatgccg ccggtatcag cttcccgtac
    47101 ccgcaaatgg atgtgaactt taagcgggtg aaagaagaca aagctgcgta atcaacgctg
    47161 aggccagata atactctctg gcctctctct tattagttat tctgattgcc aattaatatt
    47221 atcaatttcc gctaataaca atcccgcgat atagtctctg catcagatac ttaattcgga
    47281 atatccaacg tgttttctta ttactttcaa ggtcttgctc ttggggcagc tatgattctg
    47341 ccgctcggcc cacaaaatgc ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt
    47401 atgattgcct tactttgcgc tatcagcgat ttggtcctga tttgcgcggg gatttttggt
    47461 ggtagcgcgt tattgatgca gtcgccgtgg ttgctggcgc tggtcacctg gggcggcgta
    47521 gtcttcttgc tgtggtatgg ttttggcgct tttaaaacag cgatgagtag taatattgag
    47581 ttagctagtg ccgaagtcct gaagcaaggc agatggaaaa ttatcgccac catgttggca
    47641 gtgacctggc tgaatccgca tgtttaccta gatacttttg ttgtactggg cagccttggc
    47701 gggcagcttg acgtcgaacc aaaacgttgg tttgcgctcg ggacaattag cgcctctttc
    47761 ctgtggttct ttggtctggc tattctcgca gcctggctgg caccgcgcct gcgcacggca
    47821 aaatcacagc gcattatcaa tctggtagtg ggatgtgtca tgtggtttat agccttgcag
    47881 ctggcgagag acggtattgc tcatgcacaa gccttgttca gttaggcgct atctgatgga
    47941 aaaataaaac agaggcgcta agcttgcctc cagagatcct gaattttggg caagtcaatt
    48001 aacacggaga gactaacgtg aagttcaaag ttatcgccct ggcggcatta atgggtatta
    48061 gcgggatggc agcgcaggct aacgaattgc cggatggacc gcatattgtc acctccggta
    48121 cggcaagtgt ggatgcggtg ccagacattg ccactcttgc gattgaagtt aacgtggccg
    48181 cgaaggatgc cgctacagcc aagaaacagg cagatgagcg cgtcgcacaa tacatttcct
    48241 tccttgaact caatcagatc gcgaaaaaag atatcagctc agcgaattta cgcacccagc
    48301 cagattatga ttatcaggat ggtaaaagta tcctgaaagg ctaccgcgct gtgagaacgg
    48361 tggaagtcac gctccgtcag ttagacaaac tgaattcctt gctggatggc gcgctgaagg
    48421 cgggtcttaa cgaaattcgt tcagtgtcgc tgggcgtggc gcagccggat gcctataaag
    48481 acaaagcgcg taaggcagcg attgataacg cgattcatca ggcgcaggaa ctggcgaacg
    48541 gctttcatcg taaactgggg ccggtatata gcgtgcgcta ccatgtttcc aactatcagc
    48601 ccagcccaat ggtgcggatg atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt
    48661 acgagcaggc cgctattcag tttgatgatc aggtcgatgt ggtcttccag ttagaacctg
    48721 tggatcaaca acccgctaaa acacctgcag cacaataata cttgtagccc gacgtattcg
    48781 tcgggctaat gcctttaccc gatattgcct cctttttctg taattttctg taaaaccatg
    48841 acgtaatcac ctatctttgc tcgcggtttc atcaaggata ggctgactga aacggttaag
    48901 cggacaagga tgcggggagg ggggatggac atttttattt cgaaaaagat gcgcaatttc
    48961 attttattag cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca tatgacggct
    49021 tcgccatttg gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac gctatttacc
    49081 cgcaaagata ataatatcag cctcaataaa gccggtcagg aactgtatca aaaactgttc
    49141 ccggtttatc aacgactttc tgcgattgat aatgaaatcc ataactcagg gcgtcgttca
    49201 cgagaaattg tgattggcat agataatact tatccgacaa ttattttcga tcagttgatt
    49261 agtctcggag ataagtatga aggcgtgacc gcccagccag ttgagttcag cgaaaacggc
    49321 gttatcgata atctttttga ccgtcagtta gattttatta tctccccaca gcatgtatct
    49381 gcacgagttc aggaactgga aaacctgact atcagtgaat taccaccgct gcgattgaac
    49441 tttctcgttt ctcgccgtta tgaagagcgt caagagcaag agctgcttca ggagctgccc
    49501 tagctacaaa tgcgctttca aaaccgggct aacttcgaag caatgattga tgcgaatatg
    49561 cgaccttgtg ggatcaatcc caccattatt tatcgtccct acagttttat ggccaaaatc
    49621 agcgccgtgg agcgcggaca ttttctgacg gtgatcccgc attttgcctg gcgactggtg
    49681 aatccggcaa cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat
    49741 ctttattcaa tcagaaatca tcgttatacc gccacgatgc ttcagcatat tgctgaagat
    49801 cgtgacggga caaaccatta acccagcatc gagccggttg caattaaatt acggtgtaag
    49861 tcgaagacgt ggctaagatc gtggtgaata tgtccgccgg gcgcattttc cagatagcga
    49921 tgcagataat cccgatacat aggatgtgca caattatcaa tgatagtgcg ggcgcgttga
    49981 agcggggaaa gaccgcgcag atcggcgatc ccttgttcgg tgatgatcac tttgacgctg
    50041 tgttcgttgt gatcaacatg gctgcacatt ggcacgacgg ttgagatctt gccttcttta
    50101 gcaatcgacg gagccataaa gatcgacaga tacgcgttgc gttcaaaatc accgctgccg
    50161 ccgatgccgt tcatcagatc gaccccggct acgtgcgttg agttggcatg cccgtaaata
    50221 tcaaactcca ggccgacgtt cagagcgatg acgcccagac gacggatgat ttccgggtta
    50281 ttggaaatct cctgcggacg caacacaatg cggctggcaa agtaatccat attgtcgtaa
    50341 atcttgcgca gggaatcggc cgagattgtc aggctggagg cgctggcccc gctgattttg
    50401 ccggtttcca gtaaatgcac caccgattcc tgtagcactt ccgaatacat cataaacgga
    50461 ggaatttccg ggttttcccc cagacgcgcc attaccgcat tattgatatt gcccacgcca
    50521 ctttgcagcg gcagaaattc cggcggaata cgcccatgcg ccatttcctg caataagaac
    50581 gtgaccacgt tatcggcaat ctgctggcac atgggatttt gcttatccag catattaccg
    50641 gcgtcgggca agttggtttc cacgacggcg acaatctttt tcggatcgat ttgcacatag
    50701 cgggtaccga cgcgatccat tgcatggaag atcgacacgc tattgcgccg cggtggcgcg
    50761 ccaggaatca caatatccgc cagttctgca acgcgcggat cgtgatagtg attgagttca
    50821 atgatcactt tcttcgctcg cagcagccag gtcggcgcat taccgatccc gctggttaac
    50881 cagactcgac catctggtgc cagtgccgat gcttcaatga cggcaacatc aatgtcgccg
    50941 aagaaaccgt aattgaccat ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc
    51001 gcgccctgat tgatcttttt acgtaaaccg gacgatgttt gatatggcgc acgccaggaa
    51061 acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc
    51121 aggcgaattt gatacggctt tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg
    51181 gtgggtaggg ctttcggcga acccgctggg gtaaagccgc taaatgccac catgtcgtta
    51241 tgctggataa tttctgccgc ttcatcggcg gtcatccttg tccactgagt ttccattttt
    51301 actccttcgt tcgcagggat taatgaccaa cgaaattagg tttacgtttt tcgaggaaag
    51361 cgttcatccc ttcctggtaa tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac
    51421 gttcaaattc atcggagttc atggtgtgtg cttcgcccag tacacgcagc tcttctttga
    51481 taacggcaat ggctaacggc gctttctcag agatgtggtg cgccatttgt aaggtgaaat
    51541 cttccagttc ttccacttcc acaacatggt tgaggatgcc gacagccagc gcgcgctggg
    51601 cggtgatggg cgaagcggta aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg
    51661 tcaggttgtg aatgccgacc aggttatacg ggacgccgag gtttacaggc gtcattgaga
    51721 aggttgaggt actggcggcg ataatcagat cggaactcat gatcatttca aatgcgccac
    51781 cccaaacact accttccacc atcgaaataa tcggtttcgg gaatttttgg atcatgcggg
    51841 tgatttgacg caatggatca tcataggaga gcggatcgcg accgccagac ggcagttcgt
    51901 gaatatcgtg acctgcggag aagactttgg atccactcgg cgcgcgcaaa atgatacagc
    51961 gaatttccgg ccggttgaga tcgcttaacg cctgcataag atcatcaata aagactttac
    52021 ttaaggcatt aagttttcgg ccatagttaa actcaatgac cgccactttg ttgatagtga
    52081 caacgttaac atactgataa gacataaaaa ttcctttaat caaaatattg cgtctggata
    52141 aattcactga gctgccgcag gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa
    52201 agcgtctggc ggtaatagcg atcgaaatct tcattcgcga acaggtgatt cagtacttct
    52261 tcttcggtct gcttacgcag ccattccacc gattgttgtt gccgcacttg ttgtaaacga
    52321 ccactggcag ttagcgcggt tttgaagtcg atgatggcgt gccagatctc atcgattcca
    52381 cgtttttcca gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta tttacgtcgc
    52441 agaatatgca gggcactctc gtacatatgc cgggcaatgg cgacattggt atggttatcg
    52501 ccatcgtctt tgttgataac gatcagatca gccacttcca tcagcccttt tttaatgccc
    52561 tgcagatcat cgccgccacc ggcaatttgc aacgagataa aacagtccac catgcgggcg
    52621 acttctgttt ccgactgccc gacgccaacc gtttcgacaa tcactacgtc ataacccgct
    52681 gcttcgcata acagcattaa ttcccgcgct cgctgactgg caccgcccag atgaccggag
    52741 gatggtaccg ggcgaataaa cgccgcttcg gcacgcgcca ggtcattcat gcgggtttta
    52801 tccccgagaa tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat aaccgcgacc
    52861 tttaatccct ctcgaatcaa caacatgcca aaggcctcaa gaaaggtact tttccccgcg
    52921 ccgggggtgc cggtaacgcc cagtcgcagg gtgttaccgc agtacggcat aatggcatca
    52981 agcagctgcg tacttagtgc ctgatgacgc gggtgacggc tttccaccag cgtcatggcc
    53041 tgggcgagtg tggcatgctc accctgacgt aagcggcgaa tactttctgc cagcgtggct
    53101 tcattaatca tgatgctggc ttatcagatt cagtacgtcg cgcacactgt cgagcatagg
    53161 tgtacctgga ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg
    53221 tggaatgacg ccaccggcga ctacgcagat atcttcgcgt ccccattttt tcagcgcttc
    53281 gaccagttcc gggatcagcg ttttatgacc ggcagccagt gaggatgcgc ccactacgtg
    53341 aacgtcattt tctactgcca ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa
    53401 atctacgtcg aaaccgagat cggaataggc gctggcgatc actttcgcgc cgcgatcgtg
    53461 tccatccagg cccattttgg cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa
    53521 ctgctccgtt tgcgcaacaa tggcatcgaa ctcggaggcc gatttctcag attgatgata
    53581 gctttgcgca atcacgccgg taacacactg gcttggcacc agataacggt cgaaagcggc
    53641 ttccagcgca tcggaaattt cacccagggt ggcgcgaacg cgagcggcat taacagcggc
    53701 agccagcagg ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac
    53761 ggcggcatca tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac
    53821 catcacgttg tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt
    53881 gacaccaacg atgacacgct tgccctggtc gatcagcgac tgttcgcgcg ctgaggcctc
    53941 ttcgatcatt cgttttggca gacctgcttc gatcgctttc gccatgccac cggcttcgtc
    54001 gatctgttgg ataatagctc tggcttgttt gacgatttga tcggtcagcg actcaatgta
    54061 ataggatccg gccagtggat cgacggtgcg gcagagttct gattcttcct ggatgatgat
    54121 ctgggtgttg cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa
    54181 ggcgttggta tgcagtgact gagtaccgcc cagcgtcgca gccagcgctt caatggtggt
    54241 gcggataacg ttgttatacg gatcctgttc agtcaggctc cagcctgagg tctggcagtg
    54301 ggtacgcagc gccagtgatt tcgggtcctg tgcgccaaat ccactgaccg cttcgctcca
    54361 taaataacgt gccgcacgca acatggcgac gttcataaac agatccatgc cgatgccgaa
    54421 gaagaacgac aggcgaggag cgaagtcatc aattttcagt ccggcagaga ttgctgcttt
    54481 gatgtactca atcccatcag cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc
    54541 ttcacccatg tggtaaccgc tgatactgat ggtattaaat cgcggcatgt tgccggaaca
    54601 ccaggcgatg atgtcggcga taatgcgcat tgacggtttt ggcgggtaaa tataggtgtt
    54661 gcggcagagg tactctttga gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt
    54721 aacaccttgc tcttccgcgg cgatgatata aaacgccagt actggtagca ctgcgccatt
    54781 catggtcatc gaaaccgaca ttttatccag cgggatctgg tcgaacagga ctttcatatc
    54841 ttccacggtg tcgatagcga cgcccgtttt gccgacgtca cccgccacgc gtgggttatc
    54901 ggagtcgtag ccacggtggg tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc
    54961 ggccaggtta cggcgataaa aagcgttgga ctcttttgct gttgaaaaac cagcatactg
    55021 acggatggtc cacggttggg cggtatacat agtggcacgc gggccacgaa cgtagggcgg
    55081 caaaccagga agggtacctg tcacctccag attatcgaga tcggcttcgg tatacagcgg
    55141 cttgatggcg atcccttccg cggtttgctg aaccagcgag tcgacagttt tctcccgacg
    55201 gctcaattcc ttgttggcaa gctgttgcca ctcctgctcg ttagacatta tttgctccgt
    55261 aaaacgcaat caaaatgagg gtatttgcgc ttacggtgag ctttttacgt gggtttgtcg
    55321 ccagcaaaaa aaatgcattc agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg
    55381 atgtgattca aggaatacaa aaagaagtga ggataacgcc tgatatgtgc tatcaggcat
    55441 tatttgatgg attaatcctg acgaaggact ttgtgaccat aatcgagcaa cgcatcagtg
    55501 actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat ccggcgttga
    55561 aatagccctg gcgttaagtc aatcagttca ccgctggcta gttctttctc gatttgcagg
    55621 tgcgggatca tacagcaggt ggtgccctgg cgagcaagtt gcacgaacgc ttctgaagaa
    55681 ttaacgatat ggcagggcac gctgcctgga ggcagatcga agttttgctg caaaaaggcc
    55741 tggtgcatat cgtcaagatg gtcaaacgcg accactggcg ctttcagtaa tgccgaacgc
    55801 gttacgccgt tagggaaata tttttcggca aagggttttg agctgacgaa cagatagtcg
    55861 agcgcaccaa gtttatcgac aagacaactc ggcagcgcct gatgttgaat actcaccgcg
    55921 ccgaccactt cgccgcgacg cagacgttcc tgagtgcggg tttcatcttc tacctgcaag
    55981 ttgaggcgga taggcgaatc agccaataca ggagccagtg ccggaagcaa ccacgtcgcc
    56041 agactgtcgg cattgaccgc cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg
    56101 cccagccact cttcttccag caactccacc tggcgcagca gtgccagcag tttttgccct
    56161 tgttccgtcg ggcgcggcgg tacggtacgc accaacagcg gctgcccgaa catattttcc
    56221 agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc
    56281 tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta gtccgggcgt
    56341 ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca caattttgtg
    56401 tcaggttgca tataccgcag ccgtcggcgg gtaacatttg tatcaatggt taaacgcgca
    56461 gcttaaaaga tcagggattt gcgctattgc atttcgctta gcaacttcgc gccctggtta
    56521 tgttgaatgg cgtggcgtta ttgcctctgt tcgcaccgtg gagcggcata cccagaccgt
    56581 tgtatgcttt atttgcgggg cgaaagggga tgcccgccat tgcgcgatat tttatggatg
    56641 agttaaccac gcggcttgcc aacggggtct gaatcgcttt ttttgtatat aatgcgtgtg
    56701 aaatttcata ccacaggcga aacgatcatg acgcaggatg aattgaaaaa agcagtagga
    56761 tgggcggcac ttcagtatgt tcagcccggc accattgttg gtgtaggtac aggttccacc
    56821 gccgcacact ttattgacgc gctcggtaca atgaaaggcc agattgaagg ggccgtttcc
    56881 agttcagatg cttccactga aaaactgaaa agcctcggca ttcacgtttt tgatctcaac
    56941 gaagtcgaca gccttggcat ctacgttgat ggcgcagatg aaatcaacgg ccacatgcaa
    57001 atgatcaaag gcggcggcgc ggcgctgacc cgtgaaaaaa tcattgcttc ggttgcagaa
    57061 aaatttatct gtattgcaga cgcttccaag caggttgata ttctgggtaa attcccgctg
    57121 ccagtagaag ttatcccgat ggcacgtagt gcagtggcgc gccagctggt gaaactgggc
    57181 ggtcgtccgg aataccgtca gggcgtagtg accgataacg gcaacgtgat cctcgacgtc
    57241 cacggcatgg aaatccttga cccgatagcg atggaaaacg ccataaatgc gattcctggc
    57301 gtggtgactg ttggcttgtt tgctaaccgt ggcgcggacg ttgcgctgat tggcacacct
    57361 gacggtgtca aaaccattgt gaaatgatct gacgggggaa cctcccccgt taaaaaaatt
    57421 ctcttcatta aatttggtga catgtgtcac gtttttacct ggatattgtc gattgctcta
    57481 aataaatcct ctaaaccagc atattcaacc aagaaatacc tttgcgtgat atttcctcaa
    57541 catcgcgacg caaacgttca tattgccgca atattatttt ttgatatgtt gaaaggcgga
    57601 tgcaaatccg cacacaacat ttcaaagaca ggattgggta aatggcaaag gtatcgctgg
    57661 agaaagacaa gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc
    57721 ttcgtgcagc tggttacacc aacatcgaat ttcacaaagg cgcgctggat gatgaacaat
    57781 taaaagaatc catccgcgat gcccacttca tcggcctgcg atcccgtacc catctgactg
    57841 aagacgtgat caacgccgca gaaaagctgg tcgctattgg ctgtttctgt ataggaacaa
    57901 accaggttga tctggatgcg gcggcaaagc gcgggatccc ggtatttaac gcaccgttct
    57961 caaatacgcg ctctgttgcg gagctggtga ttggcgaact gctgctgcta ttgcgcggcg
    58021 tgccggaagc caatgctaaa gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt
    58081 ttgaagcgcg cggcaaaaag ctgggtatca tcggctacgg tcatattggt acgcaattgg
    58141 gcattctggc tgaatcgctg ggaatgtatg tttactttta tgatattgaa aacaaactgc
    58201 cgctgggcaa cgccactcag gtacagcatc tttctgacct gctgaatatg agcgatgtgg
    58261 tgagtctgca tgtaccagag aatccgtcca ccaaaaatat gatgggcgcg aaagagattt
    58321 cgctaatgaa gcccggctcg ctgctgatta atgcctcgcg cggtactgtg gtggatattc
    58381 cggcgctgtg tgatgcgctg gcgagcaaac atctggcggg ggcggcaatc gacgtattcc
    58441 cgacggaacc ggcgaccaat agcgatccat ttacctctcc actgtgtgaa ttcgacaacg
    58501 tccttctgac gccacacatt ggcggttcga ctcaggaagc gcaggagaat atcggcctgg
    58561 aagttgcggg taaattgatc aagtattctg acaatggctc aacgctctct gcggtgaact
    58621 tcccggaagt ctcgctgcca ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc
    58681 gtccgggcgt gctaactgcg ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg
    58741 cgcaatatct acaaacttcc gcccagatgg gttatgtagt tattgatatt gaagctgacg
    58801 aagacgttgc cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc
    58861 gtctgctgta ctaattcccc ttctctgaaa attaacgggc aggtcactga cttgcccgtt
    58921 tttttatccc ttctccacac cgttgccgcg ttgcgcattc tactttgcga gtctgctcca
    58981 gaaaataaat caaagtcaga tcaatgcgtt gtgtttattt aatggggatt tacgatggcg
    59041 ggacagcatg gggctgtaac ggtgaagccc tcaccgaagc gagggcttaa aggagaaggg
    59101 ttatgatgcg acttgtcatc atactgattg tactgttact gataagtttc ggagcttatt
    59161 aacagtcagt ctcaggggag gagtcatcct cccttaccct tactcactaa attaggtcaa
    59221 agaatcaacg atgtcaatca gggcgatgcg gggtgcatcg ccctgaaccg aactttttga
    59281 tagccacttc atcgcccacg tttactgccg taattaatcc cttattcctg caatgtatgt
    59341 ctgccttaca tgacgtttta cgagtgcgct cgaagaaaag atttaaaggt agatcaatgc
    59401 gttgtggtta tttaaagaga gtttacgatg gcagggcagc atggggctgt aacggtaaag
    59461 ccctcaccga agcgagggct tgaaggagaa gggttatgat gcgacttgtc atcatactga
    59521 ttgtactgtt actgataagt ttgccagctt attaacagtc agtctcaggg gaggactcat
    59581 cctcccttac ccttactcac taaattaggt taaagaatca acgatgtcaa tcagggcgat
    59641 gcaggtgtat cgcccttacc actcccagac tttcgacggt gtaaccaccg caggaagagg
    59701 gatatcccac tcttcaacgg ggagtttttc caccaactga caatcatgcg cataacccac
    59761 cggttgcgtt ttatagtgct gccagttttg taaggtccga tcataaaaac caccgcccat
    59821 tcccaggcgc tgaccgtact catcaaaggc gaccagcggt gtgatcagca cgtctaatcg
    59881 ggaaaggggg agcacgtcac gcacatccag ttttggctca tggattttca acctgttcat
    59941 caccagttcg ctttgcggat ggtaattcag gaacagcaaa ttaccggcac taaagggatg
    60001 caaaactgga agatatacgc gcttaccggc gcgccagagt tgttctataa gcggctgggt
    60061 gtcgagttca ccatcaaaag agaggaatac agcgaccgta tgcgccatca ccaccggggg
    60121 ataagtcatc atccgggtag cggcttgttg acccatttcc tgctgttgtt ccggcgttaa
    60181 cgcacgacga cgttgccgaa tcattttgcg gatttcttgt cgggataatg tcagtgggag
    60241 ttctggtagt tgcgtcatgg ctggtgccag ataagaaggg aatctccgag atgccgccgc
    60301 aggctgtaac ccttgaaccc ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc
    60361 tcggacggac cgagcatgct caccaaccgc ggagcgccac attcttgtgg tatgaaatat
    60421 cggctcaggg gactggcccg cttgcgaaca tctcagagaa attttgtctt cacggttact
    60481 ctaccacagt aaaccgaaaa gtgtcattca aagttttggt tagttttttc ggtgatgcga
    60541 ccttgttcaa gtaacgcttg ttctatggtc tgctgcagca tccgaatacg ctgttccata
    60601 cttgccgcgt agtcacgagt cttcgctttt tcttgcgcta actcatagct gatattcaat
    60661 gcggcaatga agaccaactg ttcagtattt gtgactctag tgcgttcttt cagatcttgc
    60721 aaccgttggt tcagatcgtc cgctgcctga ttcaacgcat ccctttggtc aggcgggcag
    60781 ttcacacgca gtgaacggcc aaaaatttgg atatcgacgg gttgtgcaga catgccacct
    60841 tcctgctgat tgactgcgct gccttcgtct ttagacccat ggtctgcgaa ggggcgacac
    60901 tatagctacc ctgatgagaa gagacaagcc cttttctggt ccaccagggc ccaaagtggt
    60961 agcatatcat gaatattcct ccctttgacg acgaatgctt atgtctatac agaacgaaat
    61021 gcctggttac aacgaaatga accagtatct gaaccaacaa gggacgggtc tgacccctgc
    61081 tgagatgcat ggtttaatca gtgggatgat atgtggcggt aacgatgaca gctcatggct
    61141 accgctactt cacgacctga cgaacgaagg catggctttc ggtcatgagc tggcacaggc
    61201 attgcgtaaa atgcactctg ccaccagcga tgccctgcag gatgacggct tcctttttca
    61261 gctttatctg cctgatggcg atgatgtcag cgttttcgat cgggctgatg cattggcagg
    61321 ttgggtcaat cacttcctgc ttggtcttgg tgttacgcaa ccgaagctgg ataaagtgac
    61381 cggcgaaacc ggtgaagcta tcgacgatct gcgtaacatt gcgcaactgg gttacgacga
    61441 agacgaagat caggaagagc ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt
    61501 tgccgcgctg ttatgccacg acacctttac tcatccgcaa ccgaccgcgc cagaagtaca
    61561 aaaaccgact ctacactaaa aacaaaaaac gtaaggagag tgttatgagt gagatatccc
    61621 ggcaagagtt tcagcgtcgc cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg
    61681 cgctgatttt tgctgcacca gaagtaacac gtagcgccga cagcgaatac ccctatcgtc
    61741 agaacagtga cttctggtac ttcaccggct ttaacgaacc ggaagcggtg ttggtgctga
    61801 ttaaaagcga tgacactcat aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg
    61861 cggagatctg gtttggccgt cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg
    61921 accgcgcact ggcattcagc gaaattaatc agcaacttta tcaactactt aatggtctgg
    61981 atgtggttta ccatgctcag ggcgaatatg catatgctga tgaaatcgtg aacagtgcgc
    62041 tggaaaaact gcgtaaaggt tcgcggcaaa atctcaccgc accggcaacg atgatcgact
    62101 ggcgtcctgt tgttcatgaa atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc
    62161 gccgcgcggg agaaatcacc gccatggcac atacacgggc gatggaaaaa tgccgtccgg
    62221 gaatgttcga gtaccatctg gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc
    62281 gctatccgtc ctataacacc attgtaggca gcggtgaaaa cggctgcatt ttacactaca
    62341 ccgaaaacga gtgtgaaatg cgcgacggcg acctggtgtt gattgacgca ggctgcgaat
    62401 acaaaggtta cgcaggcgat atcacccgta ccttcccggt caacggcaaa ttcacccagg
    62461 cccagcgcga aatctacgac attgtgctgg agtctcttga aaccagcctg cgcctgtatc
    62521 gtccgggaac ctccattctg gaagtcaccg gtgaagtggt gcgcattatg gttagcggcc
    62581 tggtaaaact cggcatcctg aaaggtgata ttgatgaact gatcgctcag aacgcccatc
    62641 gtcctttctt tatgcatggc cttagccact ggttaggact ggatgtccat gacgttggcg
    62701 tttatggtca ggatcgctcg cgcattctgg aaccggggat ggtactgacc gtagagcctg
    62761 gtctgtatat cgcgccggat gcggatgtgc cagaacaata tcgcggtatc ggcattcgta
    62821 ttgaagacga cattgtgatt accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga
    62881 aaaagccgga agaaatcgaa gcgttgatgg ctgctgcgag aaagcaatga gcgtaatcat
    62941 cgtcggtggc ggcatggcgg gcgcgacgct ggcgctggct atttcccggt taagtcacgg
    63001 ggcgctgccg gtacatttga ttgaagcgac tgcgccagag tcacatgctc atccgggctt
    63061 tgatggacga gcgatagcgc tggcggcggg tacctgtcag caactggcgc gcatcggcgt
    63121 ttggcaatct ctggcggatt gcgcaactgc catcaccacc gtgcatgtca gcgatcgagg
    63181 tcacgctgga tttgtcaccc tcgccgcaga agattaccaa ctggcggcgc tgggacaggt
    63241 tgtcgaattg cacaatgtcg ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt
    63301 aacgctgcat tgccctgatc gcgtggctaa cgttgcccgt actcagagtc acgttgaagt
    63361 gacgctggag ggtggcgaga cgctgacggg ccgcgtgctg gtagccgctg atggcaccca
    63421 ttcagcgtta gccactgtgt gcggcgttga ctggcagcag gagccttacg aacaactggc
    63481 tgtgattgcc aacgttgcga cttccgttgc gcatgaaggg cgcgcttttg aacgctttac
    63541 gcaacatggc ccgctggcga tgttgccgat gtctgacgga cgctgttcgc tggtctggtg
    63601 tcatccactg gaacggcgcg aagaggtgct gtcgtggagc gacgagaagt tttgccgtga
    63661 actccagtcg gcctttggct ggcgacttgg gaaaattacc cacgctggta aacgcagtgc
    63721 ttatccgctg gcgttaaccc acgccgccag atctattacc catcgtaccg tgctggtggg
    63781 caatgcggcg caaaccctgc acccgattgc cgggcaaggg ttcaacctcg gtatgcgaga
    63841 tgtcatgagt cttgcggaaa ccctgactca ggcgcaggag cgcggagaag acatgggtga
    63901 ttacggcgta ttgtgtcgtt atcagcagcg tcgacagagc gatcgcgaag caaccattgg
    63961 cgtcacggac agccttgtac atctttttgc caaccgttgg acgccactgg ttgtcgggcg
    64021 caacatcggg ctgatgacga tggaattatt caccccggca cgcgatgtgc tggcgcagcg
    64081 caccctcggt tgggtggcgc gttgaaggcg gttattaagg aataaacaat gcaaagtgtt
    64141 gatgtagcca ttgttggcgg cggcatggtg gggctggcgg ttgcctgcgg cttacagggt
    64201 agcggcttac gcgttgccgt actggagcag cgcgtaccgg aacctctggc ggcggatgca
    64261 ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg aaaaattact cacccgtctt
    64321 ggcgtctggc aggacattct ctctcgccga gccagctgtt atcacggtat ggaagtgtgg
    64381 gacaaagaca gttttggtca catctcgttt gacgatcaaa gcatgggcta tagccatctt
    64441 gggcacatcg ttgaaaactc agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg
    64501 tcagatatca ctcttttggc ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa
    64561 accttcctga cgctgaaaga tggcagtatg ttaacggcgc gtctggtgat tggcgcggac
    64621 ggcgctaatt cctggttgcg caacaaagcc gatattccgc tgactttctg ggattatcag
    64681 catcacgcgc tggtagcgac catacgcacg gaagaaccgc atgatgcggt ggcgcggcag
    64741 gttttccatg gcgaaggcat tctggccttt ttaccgctta gcgatccgca tctttgctcg
    64801 attgtctggt cactgtcgcc agaggaagcg cagcggatgc agcaggcaag tgaagacgaa
    64861 tttaatcgcg cgttaaatat cgcttttgat aatcgcctgg gcttatgcaa ggttgagagc
    64921 gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc agtttgccgc gcaccgtctg
    64981 gcgctggtgg gcgacgccgc gcataccatt cacccgctgg cggggcaggg ggtaaatctt
    65041 ggctttatgg atgctgcaga gctgattgct gaactgaaac ggttgcatcg tcagggtaaa
    65101 gacatcgggc agtacattta tctgcgtcgc tatgagcgta gccgcaagca cagtgcggcg
    65161 ctgatgctgg ctggtatgca gggattccgc gatctgtttt ctggtaccaa tccggtgaaa
    65221 aaactgctgc gtgatattgg tctgaaactg gccgacacgc ttcctggcgt taaaccacaa
    65281 cttatccgcc aggcaatggg attaaacgat ttgcctgaat ggctgcgtta aaaatttctc
    65341 ttctgctgtt tatttgatac ccatcacact ttcatctccc ggttttttcg ccgggagatt
    65401 ttcctcattt gaaataaact aatttcacct ccgttttcgc attatatttt ctaatgtcac
    65461 gaatttttga tttagtgttt tttgacattt tttcagctct taatattgcc ttattcaaat
    65521 taactttctc atcacatcat ctttatatag aaacttgtgt attttttggt tttttattct
    65581 gttgcgattt tggcgttttt taaccataag ctaatgtgat gatcaatttt accttatggt
    65641 taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg gggaccggga
    65701 gtggctccga tgctgggttt cgtggtgata atttcaccat gaaaaagttg tcagccccgc
    65761 ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca cacgctttgc
    65821 ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc
    65881 gacgaacatc atgaggtacg taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc
    65941 gtcgatcttc gcggcagccg cacccgggag tttctgcgtt atctgctggc gaacgatgtg
    66001 gcgaagctca ccaaaagcgg caaagccctt tactcgggga tgttgaatgc ctctggcggt
    66061 gtgatagatg acctcatcgt ctactacttt actgaagatt tcttccgcct cgttgttaac
    66121 tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc tttcggcatc
    66181 gaaattaccg ttcgtgatga cctttccatg atcgccgtac aagggccgaa tgcgcaggca
    66241 aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg tggaagggat gaaaccgttc
    66301 tttggcgtgc aggcgggcga tctgtttatt gccaccactg gttataccgg tgaagcgggc
    66361 tatgaaattg cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct ggtggaaggg
    66421 ggcgttaagc catgcggtct gggcgcgcgt gacacgctgc gtctggaagc gggcatgaat
    66481 ctttatggtc aggagatgga cgaaaccatc tctccgttag ccgccaacat gggctggact
    66541 atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg aagccctgga agtgcagcgt
    66601 gagcatggca cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat
    66661 gaactgccgg tacgttttac cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc
    66721 gggactttct ccccgacgct gggttacagc attgcgctgg cacgcgtgcc gaaaggtatt
    66781 ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct
    66841 gtttttgtgc gtaacggcaa agccgtcgcg tgatttactt ttttggagat tgattgatga
    66901 gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt aaagaagccg
    66961 acggcactta caccgttggt attaccgaac atgctcagga gctgttaggc gatatggtgt
    67021 ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg
    67081 aatcagtaaa agcggcgtca gacatttatg cgccagtaag cggtgaaatc gtggcggtaa
    67141 acgacgcact gagcgactcc ccggaactgg tgaacagcga accgtatgca ggtggctgga
    67201 tctttaaaat caaagccagc gatgaaagcg aactggaatc actgctggat gcgaccgcat
    67261 acgaagcatt gttagaagac gagtaacggc tttattcctc ttctgcggga gaggatcagg
    67321 gtgaggaaat ttacacctca ccctcactct cttcgcaagg agagaggttc acaattcact
    67381 gcacgtttca ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aatagcggcg
    67441 cttttattga acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg
    67501 ttggcgcaca atcgttaaac gcgctgaccg gccagattgt accgaaagat attcagcttg
    67561 cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc cgcgctggcg gaactcaagg
    67621 ctattgccag tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc
    67681 agttaccgcc ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtata
    67741 ctccgtatca acctgaagtc tcgcagggcc gccttgaggc actgctcaat ttccagcagg
    67801 taacgctgga tttaaccggg ctggatatgg cctctgcttc tcttcttgac gaagccaccg
    67861 ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct
    67921 tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa
    67981 cctttggttt tgaagtgatt gtcgatgatg cgcaaaaagt gctcgaccat caggacatct
    68041 tcggcgtgct gttacagcag gttggcacta ccggtgaaat tcacgactac actgcgctta
    68101 tcagcgaact gaaatcacgc aaaattgtgg tcagcgttgc cgctgatatt atggcgctgg
    68161 tgctgttaac tgcgccgggt aaacagggcg cggatattgt ttttggttcg gcgcaacgct
    68221 ttggcgtacc gatgggttac ggtggcccac acgcggcatt ctttgcggcg aaagacgaat
    68281 acaaacgctc aatgccaggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg
    68341 cgctgcgcat ggcgatgcag actcgcgagc aacatattcg tcgtgagaaa gcgaactcca
    68401 acatttgtac ttctcaggtg ctgctggcaa acatcgccag cctgtatgcc gtttatcacg
    68461 gcccgattgg cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg
    68521 caggcctgca acaaaaaggt ctgaagctgc gccatgcgca ctatttcgac actctgtgtg
    68581 tggaagtggc cgacaaggcg ggcgtactgg cgcgtgccga agcggctgaa atcaacctgc
    68641 gtagcgatat cctgaacgcg gttgggatca cccttgatga aaccaccacg cgcgaaaacg
    68701 tgatgcagct tttcagcgta ctactggggg ataaccacgg gctggacatc gacacgctgg
    68761 acaaagacgt ggctcacgac agccgctcta tccagcctgc gatgctgcgc gacgacgaaa
    68821 tcctcaccca tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc
    68881 actcgctgga gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca
    68941 ccatgaaact gaacgccgcc gccgagatga tcccaatcac ctggccggaa tttgccgaac
    69001 tgcacccgtt ctgcccgccg gagcaggccg aaggttatca gcagatgatc gcgcagctgg
    69061 ctgactggct ggtgaaactg accggttacg acgccgtttg tatgcagccg aactctggcg
    69121 cacagggcga atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag
    69181 ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg cactaacccc gcttctgcac
    69241 atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa aaacggaaat atcgatctga
    69301 ctgatctgcg cgcgaaagcc gaacaggcag gtgataatct ctcctgcatc atggtgacct
    69361 atccttccac ccacggtgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc
    69421 agttcggcgg tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct
    69481 cgccgggctt tattggcgca gacgtttcac accttaacct gcataaaact ttctgcattc
    69541 cgcacggcgg tggtggtccg ggtatgggac cgatcggcgt gaaagcgcat ctggctccgt
    69601 ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcggttt
    69661 ctgcggcacc gttcggtagc gcctctatcc tgccaatcag ctggatgtac atccgcatga
    69721 tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat cctcaacgcc aactatattg
    69781 ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg tcgtgacggt cgcgtggcgc
    69841 acgaatgtat tctcgatatt cgtccgttga aagaagaaac cggcatcagc gagctggata
    69901 ttgccaagcg cctgatcgac tacggtttcc acgcgccgac gatgtcgttc ccggtggcag
    69961 gtacgctgat ggttgaaccg actgaatctg aaagcaaagt ggaactggat cgctttatcg
    70021 acgcgatgct ggctatccgc gcagaaattg accaggtgaa agccggtgtc tggccgctgg
    70081 aagataaccc gctggtgaac gcgccgcaca ttcagagtga actggtcgcc gagtgggcgc
    70141 atccgtacag ccgtgaagtt gcggtattcc cggcaggcgt ggcagacaaa tactggccga
    70201 cagtgaaacg tttggatgat gtttacggcg accgtaacct gttctgctcc tgcgtaccga
    70261 ttagcgaata ccagtaattc actgattcga ctatcttcta aaggcgcttc ggcgcctttt
    70321 tagattgtta ataaagtgcg ctttgtgtgt ttcggctgag gcgtaatata aaatattgcg
    70381 agctcaatat attaaaagag tgccaggcct gatgagcgta gcgcatcagg cacatcggct
    70441 aattacagat tctcgccgtt gctggcaatc acttctttgt accagttaaa gctcttctta
    70501 cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag
    70561 cgtttgctgt actgcccggt agtgaacgac acgcagtcga tgcagcccca cggtgtgtag
    70621 cccatcagat ccacgccatc gtaagttact gcttttttca tctcttcaat atgggcgcgc
    70681 aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg
    70741 ccaaaaccgt tttcgacaat aaacagcggc ttctggtaac gctcatacag ttcgcaaagt
    70801 gcgtaacgca gacccaccgg atcaatctgc cagccccagt cagatgcttt aacatacggg
    70861 tttggtacgc tgccttcaaa accagagatc gcatcgccgg tgccgccttc ggccttcact
    70921 gcgttggtca tgtaatagct gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg
    70981 agatcgctat cttccatttt gatgttaaat ccgcgacgct cccactcgtt caacacatac
    71041 gacgggtaat agccgcgtag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc
    71101 tgagcgaaca tcacatcgtc cgggttacat gagtagggat agagcggcac catcgccagc
    71161 atacagccga ctttcatctc caggttaatg cgacgcgcgg ctttcactgc cagggcgctg
    71221 gcgacaaact ggtgatgcag cacctgatac atcgtctctt ccgggttttc atgctcggta
    71281 tacaccacgc cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta
    71341 atttcgttga aggtcatcca gtatttgact ttgtgcttat agcgttcaaa taccacttcc
    71401 gcgaaacgta caaagaaatc aaccacttta cggttggtcc agctaccgta ttgctgcacc
    71461 agatgcagcg gcatttcaaa gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc
    71521 agttcatcga acagagagtc gtagaacttc agcccttctt cgtttggctg agcttcatcg
    71581 ccttttggaa aaatgcgcgt ccaggcaatg gatgtacgaa aacatttgaa gcccatttcg
    71641 gcaaatagct tgatgtcttc cttatagtga ccataaaaat caacggcttc atggtttgga
    71701 tagtatttgc ctggcaagac ttctttggtt atttcgcgcg gcacgccgtg tgcgccaccg
    71761 gtcagaacgt cacaaatgct cggccctttt ccgcctttgt tccagccgcc ttcgacctga
    71821 tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc
    71881 ataggctcca gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac
    71941 tttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga
    72001 cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga
    72061 cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaacgctgg atacgctgac
    72121 ggaaaaacat gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga
    72181 catctatccc ggccagacac agttttatgt gattgaattt aaatgccttt aaattgaact
    72241 cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt tttaggagat tagtctcaaa
    72301 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
    72361 tatacatgtg cgtgtacgtt tctccggagt aagttatggt tcagaagccc ctcattaagc
    72421 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggttggtgt
    72481 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
    72541 cagcgataac cagctacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
    72601 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
    72661 gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
    72721 ctccgttagc gcgcgggttg atgattgtta tctggagcct ggcattgctg gggattctgt
    72781 tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacttat ctggcgatgg
    72841 gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta
    72901 ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac gtctgcaaac
    72961 gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt gtgtgccact
    73021 ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt aggcctgata
    73081 agacgcgcca gcgtcgcatc aggcgataaa tgcctgatgc gacacagaga tttactcttc
    73141 gagcgaatac ggcagtggct cgatacacaa tgtattcgca tcgtcgcgca cgcggaagat
    73201 gctgtccggt tccatatcgt tattcatcac tacctgcacc acgacctgac catcttccag
    73261 ttttaccgca gccagcaccg taccagtacg acgccagttc tcgcccattt tcagctctaa
    73321 gtcttcacca gcttccggca gtcggctggc actacctgtc agcaaccaga gcgcgcgttt
    73381 gttggcacca cggaattttg ctcgcgccac catctcttgt ccggtatagc agcctttctt
    73441 aaagctgata ccgcccagcg cctggaggtt ggtcgcctgt gggataaact gcccgctgtt
    73501 cgcggcatca atcaccggga aacccgcttc aatgtttaat gccagccact gttggctatt
    73561 gttcagttcc gcttcaccgc gcagtttatc ggtcagcata ttggcagtag cttcatcggt
    73621 tacgatcagg aaacgttctg ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt
    73681 aactacctgt ttttctttcg aaggcagttc actaaagata tttgccagcg cggcgcgcgc
    73741 ctgaaaaccg gcaacaccaa gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga
    73801 gaacaccgca tattttttca gttcagccag ctgcggttca cgcacgctgc gccgttcaat
    73861 ccatgcaaag ccatcgccgt cgcggaacag acgtaaattg ctccacattt tacctttggc
    73921 gtcgcaatgg gcggcgagca ggtgctgatc atctgtcatc tggctgacat ctgctgtcac
    73981 ctggccctgc aaatattttt cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc
    74041 aagcgtcatc agcgtcagtg gcaaacgggc agaagccgta ggctgacggg gaggaaaagg
    74101 tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt attaataact aatggtaaaa
    74161 gagctatgag gcaatgcaag tgctttcact acgcgatctg tgccttctgc gaggcgactt
    74221 cccaaaaaat gaagccctta aattgttgtg aattaatttc gcgatcgcga ttgtgtttac
    74281 tgatattccc gtaaaagaag tcgaaaaaca cgttacaata gccgggtact ggattttcgt
    74341 gagacaggaa gaagatggac attaacaaca aagcccgcat tcattgggca tgccgccgtg
    74401 gtatgcgcga actcgatatt tcaatcatgc cgtttttcga acatgagtat gacagcttaa
    74461 gcgatgacga aaaacgcatc tttattcgtc tgctggaatg tgacgatccc gatctgttta
    74521 actggctgat gaatcacggt aaaccggccg atgcagaact ggaaatgatg gtccgactca
    74581 tccagacacg gaaccgggaa cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc
    74641 gcacagtggc tttccttgct gattcatggg ctggttgccg ctgttatttt actcatgccc
    74701 tggccgctca gttacacccc gttatggatg gtgttacttt cgctggtggt gtttgattgc
    74761 gttcgcagcc agcggcgtat taatgctcgc cagggggaaa ttcgcttgtt gatggacggg
    74821 cgtttgcgtt ggcaagggca ggagtggagc atcgtcaaag cgccgtggat gattaagagc
    74881 ggcatgatgc tgcgtttacg ttctgatagc ggtaaacggc aacatttatg gctggcagcc
    74941 gacagcatgg acgaagccga atggcgggat ttacggcgga ttttgttgca acaagagacg
    75001 caaagataaa gatgacggca aagcatatgc gctaagccgt cagtgatatc aggcgtaatg
    75061 ctctgccatt tcgttgagga tttgctcgca ccagctctga atacgctcgt cgctaaggtc
    75121 atactggtta gtttcgtcca gcgccagccc cacgaacagt tgcccgtcag caatcaccgg
    75181 tttcgggctg gtaaattcat acccttccgt tggccagtag ccgacgaact tcacgccttt
    75241 ggtcgagagt ttgtcatgca gcataccgag cgcatcgagg aaccactcgc cgtatcccag
    75301 ttgatcgcca agcccataca gcgcaacaat tttaccttcg aggttcaggt cgtcgagctg
    75361 atcccagacg gcttcccagt cttcctggat ttcgccaaaa tcccaggtcg ggatacccag
    75421 aatgagcaca tcgtactgct ccattaattt cggggagtcg tccttgaggt tatgtaaggt
    75481 caccagttct gggccgataa tatcgcggat tttttctgcc gccatttcgg tgtaacaggt
    75541 gctggaaccg taaaaaagac ccatattcat aacgtaagct tctcgattct gctgtggctt
    75601 ttgcggctag tgtatcagaa tcgctttagt cacggcacaa taaggcataa tggatcaaat
    75661 gagtgaccat aaggggcgca agtgaaacag gatctggcac gcatcgagca gtttcttgat
    75721 gctctgtggc tggaaaaaaa tctggctgaa aatacgttga acgcttaccg tcgcgatctg
    75781 tcaatgatgg tggagtggtt gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac
    75841 gatttacagg cattactggc agaacggctg gagggcgggt ataaagccac cagctcagcg
    75901 cgtttgctga gtgcagtgcg ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa
    75961 gatgatccca gtgcgcatct cgcttcaccg aaattgcccc agcgtttgcc aaaagattta
    76021 agcgaagcgc aggtcgaacg tttattacag gcaccattaa ttgatcagcc actggagcta
    76081 cgcgataaag ctatgcttga agtgttgtat gctaccgggc tgcgtgtctc tgaactggtc
    76141 gggctgacaa tgagtgatat cagcctgcgt cagggcgtag tgcgggtcat tggtaaaggc
    76201 aacaaagagc gcctggtgcc gttaggtgaa gaggcggttt actggctgga aacctatctg
    76261 gaacatgggc gtccgtggct actgaatggt gtgtcaattg acgtgttgtt tcccagccag
    76321 cgtgcgcagc agatgacgcg acagaccttc tggcaccgca ttaaacatta tgccgtgctg
    76381 gcgggtatcg acagcgaaaa gctgtcaccg catgtgttgc gtcacgcttt tgccactcac
    76441 ttattaaatc atggtgcgga tttacgcgtg gtgcagatgc tactgggcca cagcgatctc
    76501 tccaccacgc aaatttatac gcatgtcgct accgagcgtc tgcggcaact tcatcaacag
    76561 catcacccgc gggcgtgatg tctgaaaaga acgggaagat ttatgaagaa aggttttatg
    76621 ttgtttactt tgttagcggc gttttcaggc ttcgctcagg ctgatgacgc ggcaattcaa
    76681 caaacattag ccaaaatggg catcaaaagc agcgatattc agcccgcgcc cgtagctggc
    76741 atgaagactg tgctgactaa cagcggtgtg ttgtacatca ccgatgatgg taaacatatc
    76801 attcaggggc caatgtatga cgttagcggc acggctccgg tcaatgtcac caataagatg
    76861 ctgttaaagc agttgaatgc gcttgaaaaa gagatgatcg tttataaagc accgcaggaa
    76921 aaacacgtca tcaccgtgtt tactgatatt acctgtggtt actgccacaa actgcatgaa
    76981 caaatggcag actataacgc gctggggatc accgtgcgtt atcttgcttt cccacgccag
    77041 gggctggaca gcgatgctga gaaagaaatg aaagctatct ggtgtgcgaa agataaaaac
    77101 aaagcgtttg atgatgtgat ggcaggtaaa agcgtcgcac cagccagctg cgacgtggat
    77161 attgccgacc attacgcact tggcgtccag cttggcgtta gcggtactcc ggcagttgtg
    77221 ctgagcaatg gcacacttgt tccgggttac cagccgccga aagagatgaa agaattcctc
    77281 gacgaacacc aaaaaatgac cagcggtaaa taattcgcgt gaaacaacag atacaacttc
    77341 gtcgccgtga agtcgatgaa acggcagact tgcccgcgga attgcctccc ttgctgcgcc
    77401 gtttatacgc cagccgggga gtacgcagtg cgcaagaact ggaacgcagt gttaaaggta
    77461 tgctgccctg gcagcaactg agcggcgtcg aaaaggccgt tgagatcctt tacaacgctt
    77521 ttcgcgaagg aacgcggatt attgtggtcg gtgatttcga cgccgacggt gccaccagca
    77581 cggctctaag cgtgctggcg atgcgctcgc ttggttgcag caatatcgac tacctggtac
    77641 caaaccgttt cgaagacggt tacggcttaa gcccggaagt ggtcgatcag gcccatgccc
    77701 gtggcgcgca gttaattgtc acggtggata acggtatttc ctcccatgcg ggagttgagc
    77761 acgctcgctc gttgggcatc ccggttattg ttaccgatca ccatttgccg ggcgacacat
    77821 tacccgcagc ggaagcgatc attaacccta acttgcgcga ctgtaatttc ccgtcgaaat
    77881 cactggcagg cgtgggtgtg gcgttttatc tgatgctggc gctgcgcacc tttttgcgcg
    77941 atcagggctg gtttgatgag cgcggcatcg caattcctaa cctggcagaa ctgctggatc
    78001 tggtcgcgct ggggacagtg gcggacgtcg tgccgctgga cgctaataat cgcattctga
    78061 cctggcaggg gatgagtcgc atccgtgccg gaaagtgccg tccagggatt aaagcgctgc
    78121 tggaagtggc aaaccgtgat gcacaaaaac tcgccgccag cgatttaggt tttgcgctgg
    78181 ggccacgtct caatgctgcc gggcgactgg acgatatgtc cgtcggtgtg gcgctgttgc
    78241 tgtgcgacaa catcggcgaa gcgcgcgtgc tggcaaatga actcgatgcg ctaaatcaga
    78301 cgcgaaaaga gatcgaacaa ggaatgcaga ttgaagcctt gaccctgtgc gagaaactgg
    78361 agcgaagtcg cgacacgcta cccggcgggc tggcaatgta tcaccccgaa tggcatcagg
    78421 gcgttgtcgg tattctggct tcgcgcatca aagagcgttt tcaccgtccg gttatcgcct
    78481 ttgcgccagc aggtgatggt acgctgaaag gttccggtcg ctccattcag gggctgcata
    78541 tgcgtgatgc gctggagcga ttagacacac tctaccccgg tatgatgctg aagtttggcg
    78601 gtcatgcgat ggcggcgggt ttgtcgctgg aagaggataa attcgaactc tttcaacaac
    78661 ggtttggcga gttggttacc gagtggctgg acccttcgct attgcaaggc gaagtggtgt
    78721 cagacggccc gttaagcccg gccgaaatga ccatggaagt ggcgcagctg ctgcgcgatg
    78781 ctggcccgtg ggggcagatg ttcccggagc cgctgtttga tggtcatttc cgtctgctgc
    78841 aacagcggct ggtgggcgaa cgtcatttga aagtcatggt cgaaccggtc ggcggcggtc
    78901 cgctgctgga tggtattgct tttaatgtcg ataccgccct ctggccggat aacggcgtgc
    78961 gcgaagtgca actggcttac aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc
    79021 aaattatcat cgacaatatc tggccaattt agcgtcatct tctctataaa aaagagcgtg
    79081 gattgggtac aatcccgctc ttatcaccgc attttgacta gctcaataaa agaaatcaga
    79141 ccatgtttga aattaatccg gtaaataatc gcattcagga cctcacggaa cgctccgacg
    79201 ttcttagggg gtatctttga ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc
    79261 gagctggaac agccggatgt ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag
    79321 cgttcctccc tcgaagccgt tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat
    79381 gtttctggtc tgctggaact ggctgtagaa gctgacgacg aagaaacctt taacgaagcc
    79441 gttgctgaac tcgacgccct ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc
    79501 tctggcgaat atgacagcgc cgactgctac ctcgatattc aggcggggtc tggcggtacg
    79561 gaagcacagg actgggcgag catgcttgag cgtatgtatt tgcgctgggc agaatcgcgt
    79621 ggtttcaaaa ctgaaatcat cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg
    79681 acgatcaaaa tctccggcga ttacgcttac ggctggctgc gtacagaaac tggcgttcac
    79741 cgcctggtgc gtaagagccc gtttgactcc ggcggtcgtc gccacacgtc gttcagctcc
    79801 gcgtttgttt atccggaagt tgatgatgat attgatatcg aaatcaaccc ggcggatctg
    79861 cgcattgacg tttatcgcgc gtccggcgcg ggcggtcagc acgttaaccg taccgaatct
    79921 gcggtgcgta ttacccacat cccgaccggg atcgtgaccc agtgccagaa cgaccgttcc
    79981 cagcacaaga acaaagacca ggccatgaag cagatgaaag cgaagcttta tgaactggag
    80041 atgcagaaga aaaatgccga gaaacaggcg atggaagata acaaatccga catcggctgg
    80101 ggcagccaga ttcgttctta tgtccttgat gactcccgca ttaaagatct gcgcaccggg
    80161 gttgaaaccc gcaatacgca ggccgtgctg gacggcagcc tggatcaatt tatcgaagca
    80221 agtttgaaag cagggttatg aggaaccaac atgtctgaac aacacgcaca gggcgctgac
    80281 gcggtagtcg atcttaacaa tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgt
    80341 gagcagggaa ttgccttccc gaacgatttc cgtcgcgatc atacctctga ccaattgcac
    80401 gcagaattcg acggtaaaga gaacgaagaa cttgaagcgc tgaacatcga agtcgccgtt
    80461 gctggccgca tgatgacccg ccgtattatg ggtaaagcct cttttgttac cctgcaggac
    80521 gttggcggtc gtattcagct gtacgttgcc cgtgacgatc ttccggaagg cgtttacaac
    80581 gagcagttca aaaaatggga cctcggcgac atcctcggcg cgaaaggtaa actgttcaaa
    80641 accaaaaccg gcgaactgtc tatccactgt accgagttgc gtctgctgac caaagcactg
    80701 cgtccgctgc cggataaatt ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt
    80761 tatctcgatc tcatctccaa cgatgaatcc cgcaacacct ttaaagtgcg ctcgcagatc
    80821 ctctctggta ttcgccagtt catggtgaac cgcggcttta tggaagttga aacgccgatg
    80881 atgcaggtga tccctggcgg tgccgctgcg cgtccgttta tcacccacca taacgcgctg
    80941 gatctcgaca tgtacctgcg tatcgcgccg gaactgtacc tcaagcgtct ggtggttggt
    81001 ggcttcgagc gtgtattcga aatcaaccgt aacttccgta acgaaggtat ttccgtacgt
    81061 cataacccag agttcaccat gatggaactc tatatggctt acgcagatta caaagatctg
    81121 atcgagctga ccgaatcgct gttccgtact ctggcacagg atattctcgg taagacggaa
    81181 gtgacctacg gcgacgtgac gctggatttc ggtaaaccgt tcgaaaaact gaccatgcgt
    81241 gaagcgatta agaaatatcg cccggaaacc gacatggcgg atctggacaa cttcgactct
    81301 gcaaaagcga ttgttgaatc tatcggtatc cacgttgaga agagctgggg tctgggccgt
    81361 atcgttaccg agatcttcga agaagtggca gaagcacatc tgattcagcc gaccttcatt
    81421 actgaatatc cggcagaagt ttctccgctg gcgcgtcgta acgacgttaa cccggaaatc
    81481 acagaccgct ttgagttctt cattggtggt cgtgaaatcg gtaacggctt tagcgagctg
    81541 aatgatgcgg aagatcaggc gcaacgcttc ctggatcagg ttgccgcgaa agatgcaggt
    81601 gacgacgaag cgatgttcta cgacgaagat tacgtcaccg cactggaaca tggcttaccg
    81661 ccgacagcag gtctggggat tggtatcgac cgtatggtaa tgctgttcac caacagccat
    81721 accatccgcg acgttattct gttcccggcg atgcgtccgg tgaaataagc attacgttat
    81781 gcgcataacc ccggcagatg tcggggtttt ttaattgtgc tgcgcgaaag cagacaacaa
    81841 ttttcttgct tcactattgg ctgcctgcat caccatccac ggactgaacg cccacggcgt
    81901 ggcatcaata ccgtgtaata catctgctaa atcacaccat tgataatcca tcacttcatc
    81961 atcgttgatc tgtaacgcac tggtggtgcg tgcggcaaat accggacaca cttcattttc
    82021 cacaatgcca ttcggatcgg tggcgcgata gcgaaagtca ggatagatag attcaggagg
    82081 cgtaatttcc acgccaagct cataacggca acggcggatc accgcgtctt cgttgctttc
    82141 tcccagttgt gggtgcccac aaaccgagtt agtccacacg ccaggccatg cttttttgct
    82201 cagtgcgcgg cgggtaacta ataattgtcc tttggcatta aacagccaac tggagaacgc
    82261 gagatgtaag cgggtgtctg ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac
    82321 tccctgtgca ttcaataaaa tgacgtgttc cgtttgcata atttctcaca tgtaattctg
    82381 atcactcatc attatagata attgaagagt gaaatgatta accaagtgag cgtttatcga
    82441 caaccgcccg ttttgagcgg atgccgacag gtaaaaacca tttaaaaaag tagcatcata
    82501 tatttgaagc gactttcaat ggccattgca acacagtcag aaatgacagg aatagagaga
    82561 gtaaaaagca acagcagact gaatatagcg cgcattaaaa aattcatatt attctccatg
    82621 ctcatttttc ttcagcttgc gcatcagaag ccacatacgt actgtcctga cttttaaatc
    82681 tttatcagag acggattcct tcaccaggtc agcatgatgc tgatagccat taaagaagac
    82741 gttcagcaca acggcgctaa gggttgcgag cataatgccg ctatgtagca gcggttgcag
    82801 tacggccggt aacttagaaa agaaatcgtg agagagcgtt ggagtcatgc caacgccgag
    82861 actgatcgcc acaatataga ggttgtaacg gttggtggtg tagttacagc gcgacagaat
    82921 tcgaatccct gtcgccagta ccatgccgaa catcaccaga ccagcgccgc ccagcacaaa
    82981 ttgcggaatg gaggctacca gcaccgccat ttttggcacc atgccaaaca ggattaatat
    83041 aattcccgaa gaaatacaca cccagcggct atgaacgcgc gtcacgctaa ccaggccaac
    83101 gttttgagaa aatgacgtgt gggggaagct gttaaacgtg ccaccaatca ttgtccctac
    83161 gccatcgaca cgcagcccgc gaataatatc gtgcgaagag agtttacgac cgactatttc
    83221 acccagtgcc aggaacatcc ccatcgactc gataaacacg atgattaaca cggcagtcat
    83281 ggtcagaatg gaaacgggat cgaaaatcgg cataccaaac gacatcgggg tgacaatcgc
    83341 aaaccacgag gcatcatgta gcccggataa attgacttca ttcatcatcc acgaaagtaa
    83401 aaagccaaat acaatcccca gtaatacggc gacgttggac ataaatcctt tcgcatagcg
    83461 agtaatgagc aagataaaaa ttaagacggc aaaggagata cctaaataaa cgggattacc
    83521 atattgcgga ttccctttac ctccggcggc ccagtcaata cccacctgaa tgatgctaag
    83581 cccgatagaa gtaataacca caccggtaac cagtggcggg aataaaggca tcaagcgacc
    83641 gataagtggc gctaataatg tggtgataaa acccgcggcg atagtggcac caaatatacc
    83701 cagcaggccg atatccgggt tcatcccaat ggctatcatt ggtgttacag cagcaaaggt
    83761 caccgacata atcaccggca ggcggatccc cataaagcgg ccgataccga tacattgcaa
    83821 taatgtgacg atcccgcagc aaaagagatc cgagctaatg agcatcgcaa tagcttcttt
    83881 tgagaggccc agtcggtcac caatcataag aggaacagcg actgcacctg cgtacatgac
    83941 aaggacgtgt tgcagaccga gtatgatcag ctttcctggt gataatatgc ggtcaacctc
    84001 atcagttggg cggtcttgcc cagaagatga gggaagttgg gaatctatgg cgctcatcag
    84061 tgtttctcct ttcgcccgcc agagtaaaaa gcaaccgttt acgttaatga aactgtctaa
    84121 acacatgctc gtggagtgcc agtttttgcg aggaagggga gttagagaca ggaattgcgg
    84181 aggcaatcac ataatcgtgc ttaaactacg aaaatgacaa gagaacctga caatggaatt
    84241 atctgtaatt ttctcatatt gatatttcat taatcattct ggcatgtcgc actctcgcat
    84301 ttaatcgttt ttatctggat agcgctattt ggggcggcaa tgttgaaata atgaaagcgg
    84361 catagattga ggggcaataa tttgaactca gtcgcaaaat tataattaaa acggtcatgc
    84421 tacctaatat atgcccgttt attgcatcgc gaaatttgag aagacataac tcattaaatg
    84481 aaagggatgc aaatgaataa gtttatcgct gctgaagccg cggaatgtat aggctgtcat
    84541 gcttgtgaaa ttgcctgtgc ggtggcacac aatcaagaaa actggccgct gagtcacagt
    84601 gactttcgac cacgtatcca cgttgtaggg aaaggcctgg ctgcgaatcc ggtggcctgc
    84661 catcactgca acaatgcccc ttgcgttacg gcttgtccgg ttaatgctct gactttccag
    84721 tccgatagcg tacaactgga cgagcaaaaa tgtattggtt gtaaaagatg cgcaatcgct
    84781 tgcccctttg gcgtcgttga gatggtcgat acgattgcgc agaaatgcga cctttgtaac
    84841 cagcgcagtt ccggcacgca agcctgtatt gaagtttgcc caacacaggc gttacgactg
    84901 atggacgata aagggttaca gcagataaag gtggcccgcc agcgcaaaac ggcagcagga
    84961 aaagcgtcat cagacgctca gccatctcgc agtgcagcgt tgctccccgt taactcgcgt
    85021 aaaggcgcag ataaaatttc agcaagtgaa cggaaaaacc actttggcga aatctattgc
    85081 gggctggatc cacaacaagc gacttatgag agtgaccgct gtgtttattg tgccgaaaaa
    85141 gctaactgca actggcattg cccgctgcat aacgctattc cggattacat ccgtctggta
    85201 caggagggaa agattattga agcggcagaa ctttgccacc agaccagttc cttaccagaa
    85261 atctgcggca gggtatgtcc acaggaccgt ctttgtgaag gcgcatgtac tttgaaagat
    85321 cactctggcg cagtctctat cggtaatctg gaacgctaca tcaccgatac cgcgctggcg
    85381 atgggctggc gtcccgatgt cagcaaagtt gttccccgta tcgagaaagt ggcggtgatt
    85441 ggcgctgggc ctgcagggtt agggtgtgct gatattctgg cgcgcgcagg agttcaggtc
    85501 gatgtctttg atcgccatcc agaaattggc ggtatgctga cttttggcat tcctcctttc
    85561 aaactcgata aaacggtatt aagccagcgg cgagagatat tcaccgcaat gggaatcgac
    85621 tttcatctta actgtgaaat tggccgcgat attaccttta gcgatttaac ttctgaatat
    85681 gatgcagttt tcatcggcgt ggggacttac gggatgatgc gagcagatct gccgcatgaa
    85741 gatgcgcccg gtgtcattca ggctctaccg ttcctgactg cccatacccg ccagctcatg
    85801 ggattgccgg agtctgaaga gtatccgctg acggacgtgg aaggtaagcg agtcgtggta
    85861 ttgggcggtg gcgatacgac aatggattgt ttgcggactt ccattcgcct caatgctgcc
    85921 agcgtgacct gcgcgtatcg tcgtgatgaa gtcagtatgc cgggctcgcg caaagaggtg
    85981 gtcaatgcgc gcgaggaagg tgtcgagttt caattcaatg ttcagccgca atatatcgct
    86041 tgtgacgaag atgggcgttt aactgcggtg ggcctgattc gtaccgccat gggtgagccg
    86101 gggccggatg gtcgccgtcg tcctcgtccg gttgcgggtt cagagtttga attgcccgcc
    86161 gatgttctca ttatggcctt tggtttccag gcgcatgcca tgccgtggtt gcagggcagc
    86221 ggaattaaac tcgataaatg gggcctgatt caaaccggtg acgtcgggta tttacctacc
    86281 cagacgcatc tgaaaaaagt ctttgctggt ggtgatgcag ttcatggcgc ggatctggtt
    86341 gtcactgcaa tggccgcagg aaggcaggcg gcgcgcgata tgttaactct gtttgatacg
    86401 aaggcatcgt gatgaaatcg ttaattatcg ttaatccggc tgactgtatt ggctgccgta
    86461 cttgtgaagt ggcctgtgtg gtcgctcacc cttcataaca ggagttgaat gccgatatct
    86521 ttttaccccg gctgaaggta cagcgactgg actgcatcag tgcgccggtg atgtgccatc
    86581 agtgtgaaaa cgccccttgt gttggcgctt gccccgtggg ggcgctgacg atgggcgagc
    86641 aggtggtgca gaccaattct gcccgttgta ttggctgtca gagttgcgtc agtgcgtgcc
    86701 cgtttggaat gatcaccatt cagtcgttgc cgggggatac ccggcaacaa atcgtgaaat
    86761 gcgatctctg tgaacagcgg gaagaggggc ctgcctgcgt tgaatcttgc ccaacacagg
    86821 cgctgcagtt gcttactgaa agagaactca ggcgagtccg ccagcagcgt attgttgcca
    86881 gcggtgagaa tcctctgtga aagaaatctc cgcgctgaaa ggcgcggaga atctattaga
    86941 ttgcccaacc acccgcgtaa aatgcgacca gtgcggcagt aatgatcacc gtaccaatat
    87001 tcagcttttg ccattcgcgt gcaaagacgc ggcctacgac cagtgtcaca aagcccagca
    87061 taataccggt aacgatatta caagtcagaa cgatgaacac ggcacacacc aggccagcca
    87121 tagcgtcaat aaaatcattg aaatccagct tcgagacgtt acttaacatc agcaaaccta
    87181 cgtacatcag agcgggtgca gtggcgtaac cagggatcaa aaatgacagc ggtgataaga
    87241 acagaatcag caggaataac gcccccacta cggttgcggt taaccctgtt ttacccccgg
    87301 cggcggttcc tgccgctgat tcgatataaa ccgctgcggg cgctgcgccc accaggccgg
    87361 agaatattga acttactgag tcactggtca gggctttgcc gccgttgatg atctggttgt
    87421 ctttatccaa caaattcgcc tgaccggcga cggcacggat ggtgccagta gcgtcgaaca
    87481 ctgcggtcat caccaatgcc agtacactcg gaagtacagt tggctggagt gcgcccataa
    87541 tatcgaggct gaaaatcaga gacttaccat cttcgccagt caggcttggc atcgccacca
    87601 gaccgtggta tttcaccgct gggtcaaaga ttaagccgat gatcgaaatt gcaataatca
    87661 ccaacaagat accgccgggt acgcgacact tctccagacc gaagatgacc gccagcccca
    87721 gcaagctcat catcaccggg aaggaggtaa acgcaccgag cgccactggc aagccttcaa
    87781 tcgggttttt gataaccata cccacaccgt tagcagcaat cagcagcaga aacagcccga
    87841 taccgatacc tgtaccgtga gcgataccca tcggcaaatt acgtaagatc caggtacgca
    87901 caccggttac ggaaatggcg gtgaagatga cgcccatcag aaataccgcg cccagtgcga
    87961 caggaacgct aatttgttgc ccgagtacca gactgaatgc ggtaaacgcc gtcaaggaaa
    88021 tcgcgcaacc aatcgccatt ggcaaattag cccataaccc catcagcaac gagccgaagc
    88081 ccgcgaccag acaggtggca acaaacacag ctgcgggagg aaaacctgct ttgcccagca
    88141 ttcccggaac gacgataacg gaataaacca tggccagaaa ggtcgttaag ccagccagta
    88201 cttcctgacg aacggtactg ccacgagcgg taattttaaa ataattatcc agcgcgccct
    88261 gtggctttgg tgcgtccggt gtttgtagga tgtctccaga cataatgatg tcctcacgag
    88321 gatgtaaaag ttttattatt agttgcgttc gtacaccaga cgaccatcaa cgtaggtgcg
    88381 gtagatcgaa cggtcatcgc ccaacgtcat catcacgaac aatttgtcga ctaaagaaac
    88441 agagttgtca tagcgcagct gctgtagcgg agtggcggtg ggttccatca ccacgaaatc
    88501 agcctctttg ccaggtaaaa agttgccaat caaatcgtca aggcccagag atttcgctcc
    88561 gccgagcgtg gccaggtaaa acgcttcata tgccgagagg cgatagcctt gtaattgcaa
    88621 cactttgtag gcttcgttca gcgtttgcag catgttgaaa gtggttccgg caccgatatc
    88681 cgttcccatg cccactttaa ctttcttctg ccatgctttt ttcaagttga ataacccgct
    88741 gccgaggtaa aggttggagg tcggacagaa agcaatgctg gatttggttt cgctgagacg
    88801 atcccactct ttttctttga gatggacgca gtgagcaaag acacagtttt taccggtcag
    88861 gccgtactga tggtaaacat ccagataacc atcatggtca ggataaagcg atttcaccca
    88921 ggcaatttca tctttgtttt cacagagatg ggtatgtacc cacgtatccg gatattcctc
    88981 tttcaggcgt tgcgccatcg ccatctgttc aggagaagaa gtaggggcga agcgtggcgt
    89041 aatcgcatat agcagacgac catttttgtg ccagcgttca atcagttctt tgctttggtg
    89101 atagctgctt tcggcagtgt ctagcagata atccggtgcg ttgcggtcca tcatcacctt
    89161 accggcaatc atacgcatat tgatatgact ggcggcttca aacagcgcat caacggattg
    89221 cggatgaaca gtgccaaaca ccagcgccgt ggtggttccg ttacgtaaaa gctgcttgat
    89281 gaagaacgcc gacatttcgc gggcgtactc taaatcctca taacgacgtt cagtagggaa
    89341 ggtgtgttta ttcaaccact ccagcaattg ttccccatag gcccccacca tttcactttg
    89401 cggataatgg atatgtgtat cgacaaagcc cggtactatc agtttgccgc gatagtcgcg
    89461 cacgcgaata gtgtcaggaa tttgatgctt tccgtcttcc cattcgccaa accattccac
    89521 ttttccctgt ttaatgagta ataaaccatc ctcaataaac cgcagcgcag aggcaatctc
    89581 ttccgggtta tcgaccgtac gggtgacatc aataaaactg cctcgtaccg cttttaacgt
    89641 gtgttctcct gacatcattg actcctttac tgttaatcca tctcttccgc tgaggtaata
    89701 ccaggcagaa cgttttcctg tcggtagcca ccagggagga taatatttaa taaaatcgca
    89761 gttaacccgc cagcacaaat agggttttca actaatacat aaatagaggc tggcaatatt
    89821 ttaaaaattt cgggatcgta ggagacgcca agtcctaaac ctaaagaagt ggcgacaata
    89881 agtgtttctc ggcgctttaa accgttggtg atgatgatgc gaatcccagc gatggcaatc
    89941 atggaaaaca tcaacgtcat tgcgcctccc agaactgccg agggaatggt cgtgaagaag
    90001 ccgccaatca tcggaaacaa accgaggata accagcatta ccgcgatggt tcgcccgaca
    90061 taacgtgaag cgacgccagt catctgaata accccattat tttgcgcaaa cgtggttaag
    90121 ggtaatgacc cgacagcgga ggcgataaca gaaaccagac cgtctgccag cacgccgcct
    90181 ttcagtcggg actgatactc ttctccctga atgggtcggc gggaaaccat tgccgtggcg
    90241 gtgatatcgc cgacagcttc cagcacgcta agcagataaa tcgtgccaac caccaggaac
    90301 tgatggaagc taaaactaaa gccgtatttg aacggatgtg ggatggtgat taacggcaaa
    90361 ttgcgcatac tgctgaaatc caccatgccc aggcataacg acgcgatata accgacgcat
    90421 agcccaatgg cgatccctcc catgcgtagc aacggactgc gacagcagtt aaagccgatc
    90481 accacaatca aaaccaataa accaacgccg agatgttcgt aattgccgaa ggtaccgctg
    90541 cttttggcag caaatcctcc accaaaatcg ataatgccga ctttaatcag gcttaagccg
    90601 atcatcagta ccacaatacc gctgacagta ggcgtaataa cccgacgtaa atagggcaag
    90661 ataaatgaag atccgacaac cagaaatgcg ccaacgaagg agacgccgag gagcgacgac
    90721 atgattaact cttcgtgaaa accgtcgctt ttcatgctgc tgcccagcgc aatcatgacc
    90781 gtaacaaatg aaaaattgac tgactgaatt gagagtaggc cagaaccgac gatgccatag
    90841 cggtttactt gtaaccaggt accaatacca gaggcgatca tcgccattga aacaagatag
    90901 gcagttgttt cagcggaaag ctgtaaggcc gcccccacga ttaacgcggg ggttaccatc
    90961 ggaacgaaaa ttgccaacag atgggtaatg gcaccaacga gagcctgatg aaagggaggg
    91021 cgatcctcca gttcaaatat aaggtcagaa cctgcatggt ttatatcaga catcccttca
    91081 ctccttttaa agtaaatttg atcatctgaa ttagtaaaat aaattcccac gaatttgggg
    91141 cgcaggacgc cttgagaacg gcttatctca ttttcgcagt cactatttag tcatcgtcca
    91201 aacagagaaa caggccgccg aagcggccat ttattattta tatcttttcc agcgcggtga
    91261 gtattttctc cggtgtgaaa tgccattcgc gtaaccagat gccgcatgca tcgtgaattg
    91321 cggtagcaat cgccggagct gcgccattta caccgatttc cgagatcgat ttcgccccga
    91381 acgggccgac tttatcgtcg ctcggcacca gcactgcgcg gaaatcacgc ggaatgtcac
    91441 caattttcgg tgcgccgtag ctgcgtaaat cacgcgttaa cgggtgacct tcggcgtcat
    91501 aaatgatctc ttcgctcata ctgtggccga tagctcgcag ggtagcaccg tagatttgtc
    91561 ccaacgctaa ctctggattg accggtgtac cgcagtccag caaggcgtag aatttatcca
    91621 ggcggatttc acccgtacgc gtgttgacgg caacttcagc gaagtttgcg ccatacggga
    91681 aggcgaaatc aggcgtgata taactgccag ttcccaccag tgaaccaaag ccggtgccgg
    91741 tttcgccttt atgggcaata tccccgaaac taacttcgcc tttcttgccg cgcacgacgc
    91801 ccggcgttgc tagttgaaca tctgccactg gctcacccaa catttgcgcg ccgtggaaca
    91861 gaattttctc ccgcagattt tccgctgcca aacgcgccgc gttacccgag aagcaagtac
    91921 cggacgaggc atatgcgcct ttatcaaaca acgcatgatc ggtatcaccg gagataacat
    91981 gcacgtcctg cggtgggcag tgcagcactt ctgctgccag tttcgtcacc acggtatcca
    92041 gaccagtacc aatatccgca ccgccagaat gaacgataaa ggtaccgtct gattccagtt
    92101 tgatcatgca gttagcctga tcgatatccg ggatccctga tttctgcatg ataatggcga
    92161 caccacgacc gatgtgccag tcaccatttt gtggttttgg tgaagaccat tggatcatct
    92221 cgcgcccctg acgcaggatc tcttccagtg cgcagctggc ggcggaagga acggaggtcg
    92281 gcgctttacc ttcaccgatt gcaccgagaa ttttcagctc ttgcccttcg tgtacccggt
    92341 tacgttcgat aatttccagt tggtcgatct gtaactgttc agccagttcc gctaatgcca
    92401 tggtgatagc gaagttacct ttcggtgcgc cataaccctg ataagcacca tttgggcaaa
    92461 tgttgctgta gtaggtggtg acctggaaat cgacgttatc gcacggatat aacggcagcg
    92521 acagcgccgg tccgttacac ggtacggtga gtgagtggtt gccgtaaggg ccagtgttgg
    92581 cgcggaaatc catcttcact gccgtcaggc gaccatcttt tttcgctccc agtttgacgg
    92641 tgactttcgc gacgtgacga gaggtgttag caataaactc ttcttcacgg gtgtagcgga
    92701 acagtaccgg acgcccggtc acgcaggttg cccaggcgca cacttcttcc agcaggatgt
    92761 cctgtttgga accaaaaccg ccgccaactc gctctttaat gacatgaact ttatgctgtt
    92821 tcatgtccac gaggcgcgcg acctggcggc gtaagtgcca tggtacctgg gtggaggcgt
    92881 ggataaccag acgatcgccg tccatccggg taaagcagat atgtgtttca gtcgggcact
    92941 gctgcgcctg cgttgagtta taggttcgct caatgatcac atcggcatcg gcaaagcctt
    93001 tgtccatatc gccaatatga ccatgaatac tagcggcgat atttttgcga gggcgagaac
    93061 cgatcgggaa gttgatgatc atatgctcgc cgcgctgggc tgcatggctg ttatcgtctt
    93121 ccagagtatc tggcgcacca gcaacataca ccaccggttc atcgtgcacg acaggcgcat
    93181 cttccgccat tgcttcgtcg atcgacatta ccggcttaag cacttcatat tcaacctcga
    93241 tgagtttcaa tgcttcgagc gcaatttctt cactttcagc gaccaccgca gcaacgcgat
    93301 cgccgacgtg acgcattttt ttgccgaaca tacggcggtc aagcggtgac ggttccggtg
    93361 cgctctgacc ccccggggta tagtagatat cagggcaatt caggtgagta ataacgtgaa
    93421 cgacgcccgg taaggcttca gctttgctga catccagatg agtaatcagt gcgtgagcgt
    93481 gtgggctacg taacatttta atgacgcagg cgtcagccgt tacgcggtct tcaacatagc
    93541 agggtttcgc ctgcaccatt ttcgcggcat cagttttagg ataatgcttg ccaatgactt
    93601 caaggtcgtc acggaaagtc ggagcgatat caatggtggc ctgcggatta tttttacgtg
    93661 caaccgccag ttcaatgacc tgatagtatt gctgccagcc agcatcgcgg ctgaacaaac
    93721 cagaaagcgc gtcgtcgatc tcttcgcggg taggtgcggc gatgcgatcg agaagatcgg
    93781 tgataatcag agctgcagct ggatcgttat aaccagactg caccacgcca acatcaacca
    93841 ttgcctgttg aaccagactt aactcgttcc atttgcccag agattctgcg gtacgaatat
    93901 ctgccttctc taactgtgcg gcaataagca aggacgcgtt aacgatatta ccgttaaaga
    93961 tgattgcgtc agaaccggca aacccgaaac catcatcact gttgcgtaca gagtgcattc
    94021 ccatgttaaa caacagcttt tgcacgtttt cgcctggatt aacggttagc tcctgaggcg
    94081 cgccatttaa agtaaagtgg atgatcatac ggcttcctcc ccagcctgtt ggcagtcggc
    94141 atacaggtcg gctaccagaa ctcccgtgat atagcgttta taggccacgc tgccccgcaa
    94201 atcttcctgc gggaaaatgg cgttggcgac agcctgttca agtgcattgc cttccagatt
    94261 ttgtttttcg acatcatgca gacgcagtgc tttactggcg acgccatcca gcgcaattcg
    94321 cataccgtcg tggtctgtca ttgcaacggc tgccgtcacg acggttaaac ctgcctgaga
    94381 acggctaatt ttgcgggtcg cacaggtgcg atacggatct ttaatgataa tttcggttag
    94441 caggcgatcg catgggcagg ccaggtagtc ctcgattgac agcgtttcgc cgttgccaaa
    94501 aaccagttca gcatccagtg ccagcaggac gggaagcagc actgactctt cctgccgggc
    94561 ggcgatttca ccaccaatgg tcgactgatt acgaacatgg cgtgagtaaa caaaaccgag
    94621 ggcttcacgc agtgctgcag gaataaatcg cgcatcacgc agtggctgca agcgagacat
    94681 tgcgccaatc cgcagtgcac cgttatccca gtcaacccag tccagttcca gatcctgtaa
    94741 ggaaatggca atctttttat cggtacgggt tggtgtagca ttgagtttgc tgcccccggc
    94801 gaaccagacg gcttcatcct ggtagcggcg cttcagttcc agcgcctgtt cgacagagtc
    94861 gggcctgaaa aattgttcaa tcatcttctt tccttattta accctcttcc tgcgggaaga
    94921 gggcatctgt catttatgcc agcgcatcca tccgacgcca catactggca gcggcttttc
    94981 tggcttgtgc ataaatggaa tcgcaatcga agttaaactg acggtcttca tagaccatca
    95041 caccattgac catcacgctg tgaacgctgc ctgagcccat accgaaagcg atatgcccgg
    95101 cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc gcaaatggtt aaatcagctt
    95161 tgtaaccggc ttccagaagc ccaaatttcg cgccaaagtt gcggctcatc agttcgttac
    95221 cgttagtcag ggctttggca aaactgtcag gccacagcgg accacccgca tcgcgatgtt
    95281 taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg
    95341 ccagattgcg gatgtcgcta aggtgatggt tgtagccgac atggttgttc atgtttgaac
    95401 gggcgttatg caccaggaac gcatcgcgct gattgagtag ggcgatgtca tctttcgaca
    95461 agtacagccc atgagcgacc agcgttttgc tatcgatgag atcgaattgc gccagtcgtg
    95521 ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag
    95581 cgtgaatatg caaaccacgg cctgtggcgt tcacggcttc acgtagcatc tccagaccgg
    95641 catctggcac ggtaaacggc gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg
    95701 ttgctgcttt cttcgcctca tcaatctgac gggcgaagcg gatgttttct tctacacctt
    95761 cctgcaactc tttgatgccg ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca
    95821 ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga cccgccgata tacgccggag
    95881 aggcatggtg atcgataacc gatgtacatc cgctcttaat cgcttccagg gaacaaatca
    95941 gcccgctgta atagagcgac tcttcatcaa gggcgcgatc gagccgccac cagagatttt
    96001 cagcgttgag atgaaatctg ggctgggggc gatgtttgcc ataattccgc gggaaagccc
    96061 cgagtaaaaa tggttgtgcg agcagacaat tcccggcatc acaatccgac catgcatctc
    96121 tttgtagctg gcatcggggt agcgttgcgt cagggcatcg ccgatagcga caatcacatc
    96181 attttcgata gcgatatcaa cgccttcctg cactttcgcc gggtgtaact gcactgcggt
    96241 gacattcttc agaatcaaca tgattacacc tccacgcggc ccagcagata atgatgatgc
    96301 tggtggacat ggctgatgat gcggcacata tcgttcagct ccggtggtac gttgttgaac
    96361 tgaccttcgc tgtcgatgtt taacacccag ctttggttat tcagtcgtac tcgtacccgg
    96421 caatcttcca caaggaagcc tgggttgctg ctgttatcaa agtcttgcgc caggctgaaa
    96481 acggtgattt tgtctttgta tggtttaccg ttccacggac agaactgagc gcagttgccg
    96541 cattcgttac agtaagcgtc gaggtgcagc gtctggaatc tattctggaa gcctgggacc
    96601 gcaatggaga cgttggcgcg gttcgggcag acatccacac acttgctgca aacgtagtta
    96661 cattcaaggc agcgagcagc ttcctgcgcg acaaacgcat cacgatcgtc actgttcacc
    96721 agcgtgatag agatatcgcc tttacgttga tagatttccg ccggattgac gttgttccag
    96781 tatttatcgt tctggtggga acggatattt tcccgactaa ggatggcatc ggtcgcccga
    96841 cgcgcggttc cgacagcagc gacaatggag gatggtccgc gctgtacgtc gccgatcata
    96901 aagacgtcag tcagacgagt ttcgccatta tggtcgacgt ctggccagcc gtttttgtcc
    96961 agcggcacgc ccatcgcatt cagggcttca gtatcctgct gttcaccaat ggcggtgatc
    97021 aggctgtcta catgcagtgt tactgtttcg ttggtttcaa ccggacgacg acgacctttc
    97081 tcatcgggtt cgccaagcga cataacgcgc aaggttaagg tgccatcagc atcgaaacgt
    97141 tccggattat tcaggaaacg gaactccacg ccgtcgtgca acgcttcttc atactcttcg
    97201 cgccatgcgg gcatctcttg cagtgaacga cggtaaacga ccgttgcttt ttctacgcct
    97261 ggaacgcgta acgccgcacg agcgcagtcc attgcggtgt tacccgcccc gacaacgacc
    97321 acatgtttgc ccagcttgag cgctgtaccc ttgttgtatt cacgcaggaa ggggagtgat
    97381 ttcaagacat tttggttgtc gcccgccagt ttcacaccgc tatttttatc tgtgccggtg
    97441 gcaatcagaa catagtggaa gtcctgattt tttaactgct caacggttaa atcgggtgag
    97501 cagccatact caaatttcac gccgtgagcg gcaacaaaat cgatatcgtg ctgaattaac
    97561 tcagccggaa ttcggaactg aggaatgata tttttcacca cgccgcccgc attggcttcg
    97621 cgttcaaaca gcgtaaccgg atggcccgct ctggcaagga agtaacctgc tgccagaccc
    97681 gccggacctg caccaatcac ggcaaccgga tggcgtgaac cagaaccggc tggtttgtgc
    97741 cagcgttgct tatattcatc ccaacctttt tccagcgcga cttttttcag ttcgcggata
    97801 ttcagcgcac tgtcgtaatc caggcgggta cagttgtatt ggcactggtg atcgcaaata
    97861 tgaccggtaa tggcgggcag agcgttgcgt tggtagatga gttccagcgc gtcggcatag
    97921 cggtgttcgc caagcagacg gatgtattcc ggaatatctt gcttgatagc gcaggcagta
    97981 acacaggggg caacgtagca gtcggtcagc ggcaggtctt ctgccacttc aatacgctct
    98041 tctggcttcc agtgtttctg ggtgtattcc atggttaacg catctgctgc cagtctgttc
    98101 agtcgttcga cgtcaacatg gtcaagtccc caggcgtcgg agccttccag ctcgcgcatg
    98161 caggcactta agcgcaaata gccgccaggt ttcagcaggt cggttgccat agtaataggg
    98221 cgaatacctg tatcaaaaat atcgcggata gtcagctgac tggcaccacc ggaataagaa
    98281 atgggcagtt tgccgtcaaa ggcgcgagag agaactgctg caacattgat ggagagcggg
    98341 aacagcgcac ggcctgacat atacatctct tcaccaggca gtgcgccttt attgttgatg
    98401 gtgccgagag tgttagtcag ttttacgcca aagccgagtg atttttcttt tgccagtgcc
    98461 atcaggcgtt ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc aaatgactct
    98521 tcttttaagc ctatgtaacc gaaaccgcag acatcgagga tctcacgaac acgcgcgtac
    98581 cccagtaagg tcgggttaag tttcacaaag gtgttgagcc ctttttcttc cagcatgtag
    98641 cggcaaatgg cttcgatttc atgcggagga cagccgtgca tggtggagag ggtgacgcca
    98701 tgcaccatac tggtggggat gcgagcgggt aaggcttgca agctttcgcg tttttcctgc
    98761 aatccgtggc gggctaaaaa tgcgtcatcc tggagtaatt tattcagcgt atcgcgatat
    98821 tgcgcgaatt tcggatggtc agatgcgtcc atcatattgt cgatgaactg ttgcattggc
    98881 ggttgcttaa taccttcgag gttgtaaccg acgctcatat taaagatgaa cgatttaccg
    98941 gaatcagaag gttggaacat cgcttcgaga aggtgcaggg caaaccaggc tttgaggtat
    99001 tcatcccagg ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa gcactcgtct
    99061 tcggcatcaa tacagggctt ttccagctcc aggcggtcaa gaatttggac ggtttttagt
    99121 tcgatgaaac gtccgccagt cagccaggaa gtgacaatat tttgcgcgag ctgcgtgtgc
    99181 gggccagcgg cagggccgac gggagtggca caggtttcac cgaagacgct aacagtttta
    99241 ccttttacag gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg ttgttgttgg
    99301 tattcatcaa atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat aatatccccc
    99361 ataaccctct ccttagctta tacagatatc attgagtgac cggtgttgag aaatccggtg
    99421 gtttaggggg atagagcaac aatcgcacca ctcttgccag gtttggcggg aaatataaaa
    99481 aaatgtgagg cggctcgaaa cttaagggct ttttgtgaat gaactcacag gtgctaaatg
    99541 agaattaaaa tcactcctga aaattattat tgccatccca tatatcactt tttctctttt
    99601 ctcaaaatca gtgattcctt atcattatga taaagagtgc tggagggatt aaatatttga
    99661 acgcaatcgt ttaactacgt tttttaaatt ttagtatacc ttttttgcgc tgtaataaag
    99721 tcgtccggag tatcaatatc taaaattatt tctgcatttt ctacttcgac ggaataatga
    99781 gctcccataa ggagtgcctg acgcatattg gtgatattgg ggcgcttgat tgcctgcatc
    99841 aggcatggtt ttgacactaa aatcggatgg ccggggatgc cattatggag tggcagtatt
    99901 gcgccatcat ttcgtaacga ccagattttt ctgaaaatat cgatggtgag ggttggcatg
    99961 tcaccgtggg tgagaaaaca atgttctgtt tgcactgcgg gtactgcggc ctttactgat
   100021 gttagtaaac cctgcgcata atctgggttg tggataatag tgatattgct ctggttcgca
   100081 taacgttcgt gcagttcatt accacgatag ccggtgacta aaataattcg gctacaaaac
   100141 tgcaacgcat ttttgatact tgtatcaaga attgtcccct gttgccaggg taacatcatt
   100201 ttccattgcc ccattcttga tgataatccg gcagcggtaa ttatacagtc gatggctgac
   100261 atagtggttc cattatttat aggggctaat aacgcctgat aattgcgtta cttcctgatg
   100321 gattaagttg gggataattt atcccagaga ggtcataaag actcgtgaaa agtatagttg
   100381 acccatcggc attattcatt gatttaggtg cgcagaaacg tcccactgtg atttctgttg
   100441 ttggtgctgg ggggaaaacc agcttgcttt tttggctggc agaactgctc caggctagcg
   100501 gcaggcgtgt gttaatcacg acgacaacac atatgtttat gccaacgtct cactggcccg
   100561 tggttttctg tcgtgatccc gccatgcttc ctcatgcgtc tcttacatcc cccatttcat
   100621 tttgttttca cagctggaaa gcgaacctgg gaaaagtgca gggatttacg ccagaagcga
   100681 ttgatgcact ggtgcaacga ccagagtgtg acgtaattct cattgaagca gatggctcgc
   100741 gtgcaatgcc gttaaaagcg cctgatgagc acgaaccttg catacccaaa agcagttgct
   100801 gcgtgattgc tgtgatggga ggacatattt tgggcgcgaa agtgagcacg gaaaatgtcc
   100861 atcgctggtc gcagtttgct gatattaccg ggttaacacc tgatgcaacc ttgcaactga
   100921 gcgatctcgt tgcgctggtt cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt
   100981 gtcggcgagt ctggttcatt aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg
   101041 agctccttca accgctgcaa caacacgacg tagaggcaat ctggctgggc gatatacaag
   101101 aacatcctgc aatcgcgcgc agatttgtga attagcggcc cgggtattcc gggttactga
   101161 aaggatatcc attgaggttg gtatgaatat tttcacagag gctgcaaaac tcgaagagca
   101221 aaattgtccg tttgctatgg cgcaaattat tgatagccga ggctcgactc cccgccattc
   101281 tgcacaaatg ttagtgcgcg ccgatggctc tatcgtcggt acaattggtg gcggaatggt
   101341 tgagcggaag gtgattgaag agtcgcttca ggcattgcag gaacgtaagc cgcgattatt
   101401 ccatggacgt atggctcgta acggtgcgga tgctgtcggg tcagattgtg gaggtgcgat
   101461 gtcagtgttt atcagcgtcc atggtatgcg tccacgtctg gtgttgattg gcgcggggca
   101521 tgtcaaccgg gcgatagccc agagtgcggc gctattagga tttgatattg ccgttgccga
   101581 tatttatcgc gaaagcctca atcctgaact attccctcca tcaaccacgc ttctccatgc
   101641 tgagtcgttt ggtgcggcag tggaagcact ggatattcgc cctgataatt ttgtcctgat
   101701 tgccacgaat aatcaggatc gtgaagccct cgacaaactc attgaacagc ccattgcatg
   101761 gttggggttg ctggcaagtc gtcgcaaggt tcagcttttc ctgcgtcaat tgcgtgagaa
   101821 aggcgtggct gaagaacata ttgcccgttt acatgcgccc gttggttaca acataggtgc
   101881 ggaaacgccg caggagatcg ccatcagcgt gctggcagaa atattacagg tgaaaaataa
   101941 cgcgccgggt gggctgatga tgaaaccttc tcatccttcc ggacaccagc tggtggtgat
   102001 tcgcggtgcg ggagatatcg ccagtggtgt ggcgctacgt ctgtatcatg cgggttttaa
   102061 agtgatcatg ctggaagtgg aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca
   102121 ggccgtgttc gatggcgaaa tgacggtcga aggcgtcact gctcgcctgg caaccagctc
   102181 tgcggaagcg atgaaactta ccgaacgcgg attcatccct gtgatggtag atcccgcctg
   102241 ttcattgctt gatgaactga aaccgctttg cgtggtggac gctattctgg cgaaacagaa
   102301 tttgggaaca cgggcagata tggcaccagt aacaatcgcg cttgggccgg gctttgctgc
   102361 agggaaggat tgtcatgcgg taattgaaac aaatcgcggg cactggctcg gtcaggtgat
   102421 ttactgtggt tgtgcgcagg agaataccgg tgttcctggc aatattatgg ggcataccac
   102481 ccgacgggtg atccgtgctc ctgctgcagg cattatgcga tccaacgtga aattaggcga
   102541 tctggtgaaa gagggcgatg tgattgcctg gattggtgag catgaaatta aagcaccgtt
   102601 gacggggatg gtgcgtggct tgttgaacga cggcctggca gtggttggtg gttttaaaat
   102661 tggtgatatc gatcctcgtg gtgaaaccgc tgatttcacc agcgtttctg ataaagcccg
   102721 ggcgattggc ggcggcgtac ttgaggcgtt aatgatgttg atgcatcagg gcgtgaaagc
   102781 gacaaaagaa gtgctggaag tggcttaaat gaaaatggcc ggatggtatt acccacccgg
   102841 ccttaaaatt actgacaaat caccgttccg gttttgcctt caatcccttc tttcgctttg
   102901 ctcaatacgg taatcagtgc ttcacgacct gcgcgagagc gagcaaacga ggcggcagct
   102961 tccactttag gcagcataga acctttcgca aaatgacctt cttcgataaa gcgttcagca
   103021 tcacttaatg acaggcgatc gagccactgt tcattctctt taccgaagtt aatggccact
   103081 ttttctaccg cagtcagaat gatcagcata tcggcatcaa tcatttctgc taaacgggcg
   103141 ctggcccagt ctttatcgat aaccgcgctg gcaccacgca gatggttacc ttcacgaata
   103201 acaggaatac caccaccgcc aacggtgatc accacctggc ctgcttctac cagcgctttt
   103261 accgtctctt tttcaatgat atcaaccggt tttggcgagg caacaacacg gcgatagccg
   103321 cgaccagcat cttctttcag agtgtaaccc tgttttgtca gttgctctgc ttcttgctcg
   103381 gtaaagaacg aaccgatcgg cttggtcggg ttgaggaatg ccggatcgtt agcgtcgact
   103441 tcgacctgag taaccagcgt tactacaggt ttattaatgc cccgagaaag cagttcttct
   103501 cttaaggcgt tttgtagatc gtaaccaata taaccctggc ttaaggcaac acagacagac
   103561 atcggcagca ttggcgaatg cgcttcagtt tttgcggcag cttcaaaggc ctggttaatc
   103621 atgcccacct gcggcccgtt accatgagtg acgataactt catgtccctg ggcaattaaa
   103681 tcaacaattg cctgagaagt aatttttacc gctttcattt gtccggccag gtcatcgccc
   103741 agcgcattcc cgcccagggc gagaacaatt ttcttactca tttttcctca ctcatcattc
   103801 ttgattgtgt atagatccct ggcagtggtt agagcacggg agggacaaac ggtttgcgtc
   103861 gcaggaaacg acctcgcccg gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac
   103921 cacgggataa ggttctgaca atcgcgccct gacaggtaaa accctcccat ggcgagtagt
   103981 cggcgttgtc gtggagatgg cgatgctgaa tttgttggct ctgacgtggg tcgataatca
   104041 ccacgtcgcc atcggaaccg ggcgctaata atcctttttg cggccacagg ccaaacagcc
   104101 tggcgggcat tgcgctggtt aattcaacaa agcgttccgg tgttatacgt cccgtcatca
   104161 cgccactgga aaacagtaac tgcatgcggt tttccacacc gggtaagcca tttgggcagc
   104221 gactgaaatc gcctttagaa atttgcaggc gttgagccat cgagaaggtg cagtgatcgg
   104281 tcgccaccac gtcaatcgca ccatcgctga tgccacacca cagtttgtcc tgctcgcgta
   104341 cgttacgcag cggtgggcta agaatgaact tcatgccgtc ttctgtatcg taactgcgtt
   104401 cgtccaacag gagatattgt gggcaggttt caacccagac tggctggtga ttcgcacggg
   104461 caagacgcag ataatccaga cctaagccgt tagacaggtg cacgatatag agcggggcgt
   104521 taccggcaat ttgtgccagg ttaatcatgc gggcgatggc ttccgcttcg cattccagag
   104581 ggcgactcaa ggcatgatag cgcggcgcgg ttaaccctgc ggcgataaat tccgcccgct
   104641 tgctggcgat agccgcatca ttttccgggt gcacggtggt cagcgcgccg gattcatgca
   104701 gacggcgtaa tgcctgcaaa acctcgtcat cgttgagttt gtattgatag gttaaataga
   104761 gtttaaaact gctcagccct tcctcgacca tcatcgggat ttcgtcgagg atggcgtgat
   104821 taatgtgctg gatcacaccg tgaaagctgt aatcgatgac cgctttatgg gcggcataac
   104881 cacgataaac ctccagttga tggcgtaacc gacagccgtt tgggccaaat cccatatggt
   104941 caataatggt tgttgtaccg ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac
   105001 aactgcgcgc gatgccgaca tcaatattga aatgcgtatg gacatccacg ccgccaggga
   105061 aaacgtaaca gccagtggca tcaatttctt catacgggag ctgcggcgaa atattgttgc
   105121 ccaactggcg aacaatcccg ctttcaatca gcaaatcctg tttggcttgt ccatctgcgt
   105181 taacgacagt gccgtttttg atcaatacgc gcatagcaaa ctccaaaaac cctccggcga
   105241 accggagggg aataagtgaa gagtaattat tcggttgcca gccagcttaa cgggattgca
   105301 gcgtacatcg cggcacaggt caccaggtga gatttccagg ttttttcgtt aggtgcatgc
   105361 gcttcaggtt ctttacccgg gccaaagccg atcaccggaa tgccgtgacg gcccatgata
   105421 gatacgccgt tagttgagaa ggtccactta tcaacaaccg gcgctttgcc aaacagccct
   105481 tcgtaggcat tcaccagtgc tttaacggtg aagtgatctt cttccacttt ccaggtcggg
   105541 aagtagcatt cggttgggta aaccaggcca gtccaggacg gacggtcgta gttgtacata
   105601 gaaacaaccg cgttagcttt ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct
   105661 tcccaggttt cgccccaggt cagacggcgg tcaatagaga cggcgcagct atctgctaca
   105721 gcgcaacggc ttggggatgt gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg
   105781 aattcgtcat aacccagacg ttgggagagt tcttgtaatt cgccaagaat cggacccatt
   105841 ttgaaaatgg cgttgtcacc gcgttctggc gcagaaccgt ggcagctaac accctgaaca
   105901 tcaatacgaa tttccatacg accgcgctga ccacggtata cctggcagtc ggttggttcg
   105961 gtactgacca caaattccgg gcgaatgccg gattgttcaa taatgtactg ccagcacaga
   106021 ccgtcgcagt cttcttcctg cacagtaccg gtaaccagca gggtatattc atcttccaga
   106081 ccgaggtctt taatgatttt accggcataa accatagatg ccatgccgcc ttcctggtcg
   106141 gaagtaccgc gaccaccgat cagctcatca gtttccatgc cttcgtacgg atcgaagtcc
   106201 cagtttttga tgttgccaat gccgacggta tcgatatgag cgtccattgc caccagacgc
   106261 gggccgtggc cgatataacc gagaacgtta cccatcgggt cgatttcaac tttatcgaag
   106321 ccgacttttt ccatctcttc tttaatacga tgtactaccc gtttctcgtc gcagctttca
   106381 ctgggaatag caaccatgtc gcgcaggaag cgagtcatat ccgcctggta atcttttgct
   106441 ttttcaagaa tcagtttgaa tggaatattc ttagccatta tttttctcca gattttttac
   106501 tccccgtttt gcagggagca gttccaatag ggtgattaag gtgctacagc gtgtttgcct
   106561 tcccagacaa cttcgcggta gtgcttcacg tcggtgtcgc cttcagtgct gataaccagc
   106621 actacggcat ctttgttcag cgccagtttt tccatcaggc tttgacgttg cgggtgataa
   106681 tgaaccgctg cgagaacgcc caaaccgaca gcgccggatt caccggagat gatgcgcggg
   106741 tcgttgccgt acggattacc cagcacgcgc atacctaatg cggcaacgct gtcctggcag
   106801 gagatgaatt gggtggcgca gttacgtagg atttcccagc ccagcgggtt aggttcgcca
   106861 caggccaggc ctgccatgat ggtggccata tcaccgccaa cgttgacgat gtcgcctttg
   106921 acgccggagc gataaataca gtcagctttg tcaggttcaa caataatgct gtgcagattt
   106981 tgcgggctat agacgtcgac cagataaccc agcacaccac cggccattgc tccgacaccg
   107041 gcttgcagca gaacgtgcgt cggggttacg cccatttcac gcatttgctc gacggcttca
   107101 tctgccaggg ttgcgtagcc ttgcatgatc caggttggga ttttggtgta accttcccat
   107161 gccgtgtcct gtaccacttc ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg
   107221 gtatcgtcat agttcatatc tgtgacgatg cactcggcac cgaggttcag aatggcgtca
   107281 acgcgttcct gagcagaacc tttcggcatg taaatcaccg cattctgtcc gagttgctgc
   107341 gctgcccacg ccacaccgcg cccgtggttg ccgtcggtgg tggtcgcgaa agtcattttt
   107401 tcgccgatgg catttttcag gtgctcaaat gacagcgttt cgatatcaag atgatatttt
   107461 tcacacaata attgagcgat agcgtacgca ccgccgagca ttttgaaggc gttcagaccg
   107521 aatcgttttg attcgtcttt gacaagaatt ttcttcaccc caaaaaggtt tgcgagatcg
   107581 tccagcgcac aaagcggtgt tgggcgataa ccagctattt tctggtggaa ctggcgcgcc
   107641 agtttggcct ggctttgcga aaagagcggt gatgtttcgc cgttgaaaaa tttgttatcg
   107701 gcgatatcaa tcttcaatga gaaaacggac atatcctatc cttttggcct ctggaaatag
   107761 agggccgacc agctgatcgg cccgaggggt tatttcacgc gttcttgcgc ttctttcagc
   107821 agttgctcga gcagtgcacc tggtttggcg tatttacggg acaggatcat cgcggcgatg
   107881 atataaggct tccagctggc ttctttgtag gtagcgatac ggtatttttc gaatacgcct
   107941 tcagtcactt ccccttcttt gcaggatacg ccgctgatat cagctggcag gcagtgcatg
   108001 tacagggctt cgccatcacg ggtcagttcc atcatctctt cagtacaatg ccagtctttg
   108061 tgttgcgcgt tctgtgccag acactgtttt tccagtgctt ttaagccttc gtgatcgtta
   108121 gcgcgcagca attcggtacg ctgttccatc actttgtaag gtgcccatga cttcggataa
   108181 acgatgtctg catctttgaa cgcttcttcc atgctggtga cctgacggaa gctaccgcca
   108241 gatgctttag cattgttttt cgcgacttct actacatccg ggatcaggtc gtagccttcc
   108301 ggatgggcca gggtgacatc cataccgaag cgagtcatca gaccgatgat gccttgtggt
   108361 acagagagcg gtttgccata gcttggagag taggcccagg tcatggcgat ttttttacct
   108421 ttcaggtttt ccagtgaacc aaagtgttca cgtaaccatg ccaggtcagc cattgactga
   108481 gtcgggtggt caatatcgca ttgcaggttc actaaagccg gacgctgtgg cagtacaccc
   108541 tgcttgtaac cgtcatcaag tgcagcgcca acttcacgca tataggcgtt gcctgcgccc
   108601 agatacatat cgtcgcgaat accaatagcg tcggcgcaga aggagatcat attggcggtt
   108661 tcacgcacgg tttcgccgtg agcgatttgt gatttgcctt catcgagatc ttgctgtgca
   108721 aggccgagca ggttaagcgc ggaagcataa gagaagcggg tacgagtgga gttgtcgcgg
   108781 aatacggaaa tacctaatcc actattaaag actttggttg agatgttttc agcacgcagt
   108841 gcttttaatg ctgcggcaac gtccagtact tgtttcagtt catctggagt ctgttcccac
   108901 gttaacaaaa aatctttctc gtgaaggtga gaggtcagcg aattgatatc cttaatcagc
   108961 tcattaacag ttttcatcat aaagtcccgg tagtggaatg acaggcaaag tggtttattt
   109021 cactgtgact tgccaaagtg attggtgttt tcacagctat acctgggact aacaataagt
   109081 gtgccagaat tatccacggg gctgcaaaag actaatttga cggataatgt gtggggttga
   109141 tcacaaattg atgccagctt ccggttgttt gtaaaatacg aaaacgtttg gcttttttta
   109201 tcgcgttatt tatttgctgc taagtatcag aaataagata aagggacagg ctatccctcg
   109261 tcagtttgat aaactcaccg ccataattct catattgata tagaaatgtg atatctcttc
   109321 cataatttca ttagaaagtg ataaaaatct tatgttttta tcaaagccaa tttcgcctga
   109381 gttaatatga tcatgatctg tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt
   109441 ctggaaggta aagctatgga gcttgctact acgcagtcag tattgatgca aattcaaccg
   109501 acaattcagc gttttgccag aatgcttgcc agcgttttgc agcttgaggt tgagatcgtt
   109561 gatgaaaact tgtatcgcgt cgccggaacg ggcgcgtatg ggaagtttct tggtcgccag
   109621 ttgagcggca actcacgcct gctctgccac gtcctggaaa cgaaaactga aaaagttgtg
   109681 acacagtctc gcttcgatcc cctttgcgaa ggttgcgata gtaaagaaaa ttgccgcgaa
   109741 aaagcatttc tgggtacgcc tgtcatttta caggatcgtt gtgttggggt gataagtttg
   109801 attgccgtta cccacgagca acaagagcat atcagtgata atttacgcga attttctgat
   109861 tatgttcgcc atatatccac catttttgtt tcgaaacttc tggaggatca ggggccagga
   109921 gataacatca gtaaaatatt cgcgaccatg atcgataata tggatcaggg cgtattagtt
   109981 gttgatgatg aaagtcgggt tcagtttgtt aatcagactg ccttaaaaac acttggtgtt
   110041 gtgcaaaata atattattgg gaaacctatc cgtttcagac cattaacatt tgagagtaat
   110101 tttacccatg gacatatgca gcatattgtt tcgtgggacg ataaaagtga attaatcatt
   110161 ggtcaattgc ataacattca gggccgacaa ttatttttaa tggcgtttca ccaatcgcat
   110221 accagttttt ctgtagcaaa tgcacctgat gaaccgcata ttgaacaatt ggttggcgag
   110281 tgccgtgtta tgcggcaatt aaaacgactc attagccgta ttgcacccag cccatccagc
   110341 gttatggtgg ttggtgaaag cggcacgggt aaagaagttg tcgcccgtgc aatccataag
   110401 ttgagcggaa gacggaataa accctttatt gctatcaact gtgccgcgat tccggagcag
   110461 cttctggaga gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc ttctgccaac
   110521 ggtaaaacag ggttgattca ggcggcgaat acgggcacgc tgtttctcga tgaaataggt
   110581 gatatgccat taatgttgca ggctaaatta ctgcgcgcta ttgaggcacg tgaaattctg
   110641 ccgattggtg ccagtagccc aatacaagtc gacattcgca tcatttctgc aactaaccag
   110701 aatttggccc agttcattgc cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat
   110761 gttatcccga taactctgcc accgctgcgt gaacgtcagg aagatattga actactggtg
   110821 cattactttt tacatctgca tacccgtcgt ctgggatcgg tttatcctgg cattgctccc
   110881 gatgtcgtcg aaatattgcg taagcatcgt tggcccggaa acctgcgcga gttaagcaat
   110941 ttgatggaat atctggttaa cgttgttcct tcaggtgaag ttatcgacag cacgctattg
   111001 ccgccaaatc tgctgaataa tggcacaacg gagcaaagtg atgtaacaga ggttactgag
   111061 gcacacctgt cactcgatga tgcgggcggc acggcgctgg aggagatgga aaagcaaatg
   111121 atccgcgagg cgctttcacg tcataacagc aagaagcaag ttgctgatga actgggcatc
   111181 ggcattgcta cgctctatcg caagattaag aaatatgagt tgttaaacac ataacatcag
   111241 tgaatccggc ctgaattcag gccggatatc cttttacttc gttttctcgc aatccagaac
   111301 tgtatttaca atcatctgat accccgtgca gcgacaaaga tttcccgcca gtccgcgacg
   111361 aatttccgta atggttaatg gcttctcgcg tggtttcgcc agcattgccg tggtagccat
   111421 aatcaggcca ggcgtacaaa acccgcactg cactgcgccg gatttcgcat aagcctgctg
   111481 aacatgagaa agttttccgc ctttcgcttc accttccagc gtgcggatct cttttccttc
   111541 agcccaggcg gcaaggtata agcaactgtc tattgctgtg ccgtcgacca gcaccgtaca
   111601 ggcaccacat tcacccacgc agcacccttg tttgacactt agcagccctt gttcgcggag
   111661 taattccgag agcggcgtgc ctggtacggc atgaagctga aaaggcatcc cgttaatggt
   111721 gcattcgatg gtaattgttt cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg
   111781 gcttcgctaa tcactttttt ggtcatcgtc tggatgagat gcagacgaaa ctctttactg
   111841 gcccgccatg aagaacgcgg ggcgacatct tgcaggacag attcgctgat agcttccagc
   111901 gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg catgttggca gcgaatcggc
   111961 gttggcgcgg caacaccaaa tgccaggcgt aattcgctga aattgccgtt atccagtcgg
   112021 caatgtgcgg cacagccaat cgttgaaata tccattgcgt cgcgcatggc atatttaaaa
   112081 tgcgcgctgc ccacgtgttc tttcggctgg ggcggaaaat gaaaggcgac aaggatttcg
   112141 tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc cattaatcgg gacgaaacga
   112201 acaccgcgtg gggagtggat ctccagtttc gcgtcataaa tgagcgttgg cgtggcagaa
   112261 tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt acggatctgc
   112321 ggtccagcaa tggacgaggc cgcagcacat aacgccggga gatgacgttg agttatagaa
   112381 tcttctatta gctgggtaaa tgtcgttgca gagccgatac gtagcgagcc atcttccgcc
   112441 agcgtaattc cccgcagctc cgccagatta tgaatatcaa caatatggcg ataacggtca
   112501 ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg cgagcagttt ggcctgcggg
   112561 ttgtcagcca gcaggttgat ggcatcggca agggttgctg cgcgatggta agaagcaaaa
   112621 tcaaacatga tgttatcctc aaatcaatcc tgccagatgg aactcttcat ataaccgttt
   112681 tggcgtcagc ggcagtgtat tgattgcaac accggtagcc atcttcaccg cgttacgaat
   112741 agcagcggca acaggaatta ttggtggctc acccagtgac ttatgtccgt atgcggattg
   112801 cggctcattg atttcgacga acgcgctttc cagttgtggc agatccggca tggtcggcat
   112861 tttgtaatcc agcagattgg ggttacggac cacgccgctt ttcgcatcga tgatcatctc
   112921 ttcaaatagc gcccagccaa tgcccattcc cattccgccg tgtacctgac cttctgccag
   112981 tagcggatta agaatatggc ccgaatcatg aacgttgagg atgcggttga tggtgacttt
   113041 gcacagcgca atatcgaccg tcagatcaac aaaggtacag ccgaacgccg gtgggttagt
   113101 ggtggttttg atggagcttt cagcagagag ctgcccgccg cgttcagggt ggtagaaagc
   113161 gtccatcgcc aaatctttta acgacattaa cggctcttcc ggtcgttcaa ccagcacgat
   113221 atggcctttt atcagggtca gattcatcgc tgactgatgt agcatgactg cggcgtgagc
   113281 gatgattttc tcttttaata acagtgccgc actgcgcagc gcaggcgcgg caacatagct
   113341 ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg tcggtatctt gtgttgaaat
   113401 aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc accatttgcg agaagacggt
   113461 gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt
   113521 catcagaagg cgcgcgcctg ctatttctac gccgacaggc caggtgttag aggtgtagct
   113581 aaaacaggcg acgccaacgc cgcggcgtaa attgccttgc tggttctggc actctgcacg
   113641 gcgtttttcc cattcaaaga ttttccggcc tttttcaaga cactccggca accctgcgct
   113701 gtaaatacgt ttgcccgtga gcggattagc atctccttcg cgtgaggcgt tgcgtaaacg
   113761 aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca tcaagcatag actcaacggc
   113821 aaatacgact tgtggcgcgc cataaccacg catcgcacca gccgagggga ggttggtata
   113881 gcaggtcttt gaactgtaag cgtaggcaca acgaggataa aggtaagcga ctttatttcc
   113941 ccctgcagaa gcgatggagt gcccgtgaga tacataagcg ccggtgttag acagaacatc
   114001 cagactataa cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt caatagtaaa
   114061 agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg
   114121 aatgccgcca agcttgctgg tcaggaatgc cgccattggc tcttccagta catcctgttt
   114181 attaccaaaa ccgccaccga caaatggttt gatgactcgt acgcatgacc agggaatatc
   114241 cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt
   114301 aattcgcgag tcatcctcca tccacgccag cgatgttacg ctttccatgt gacaatgttg
   114361 aataacgggg gtctgatagt gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg
   114421 gacattgccc gtcgacatcg tgctttgttt cagtaaattg ccgccgttat ggattggtgc
   114481 agcgtcctct gccagcgccg cttctggcgt ggtgataacg ggtaattctt cccactcaat
   114541 gctgaccaat tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaac
   114601 ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt
   114661 gttttcgtca agtgtccatg catgcccagc tgtagcgaat gggatatcag gcacatcttc
   114721 ccaggtaaaa atcgccagta cgcctggtaa acttctggct tgttcatcat taatacttac
   114781 ggcataacca tgtgcgatag ggctacgtac atatttcgca taacacatgc ccgccataac
   114841 ataatcgtca gtatatcgtg cccgcccggt gaccttagca atggcatcaa cgcgcatgca
   114901 tgattcaccc gtagcggttg cttcccgcgc ttccatagaa atcccctcga tttgaacatt
   114961 tggttcttat ggcaaagatt tagtctaagc aggcaagatt tacgccagaa acggcgataa
   115021 gcgttaaggg aggaaggaag tgtgatgatg ttaaaaaaac acagatatga tacgaaacag
   115081 gaccctgaat aggggtagtg atacgtatca atatgagtgg cgataaaatc aatgtgataa
   115141 attaggatta ttacgcatca ggatgggaaa tagggtaaaa aagtcaggaa cttcgatacc
   115201 tctacagtga atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct
   115261 ctattacatc gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata
   115321 tggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc
   115381 tctgggtatc gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa
   115441 atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct
   115501 atatcgtatt tcgcgcacca cgggaaccag cgtaaaagag ctggcgcgac tgaacggcat
   115561 ttccccccct tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag
   115621 cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc
   115681 atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac
   115741 gacagggaaa gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat
   115801 ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg
   115861 caaccagctg cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac
   115921 ggcttacgcc cataatgaca cgatactggt aaataatggg caaagcgtga aggctgggca
   115981 aaaaatcgcc accatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg
   116041 ttaccgtgca acggcaattg atccgctacg ttacttgccg cctcagggca gcaagccaaa
   116101 atgctgatgg cgaattaatc agcagtcagc agcgcggcac ttgctaagaa gagcgtaagg
   116161 tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc
   116221 aagctctata cgagggttcg attcccttcg cccgctccaa tttatcttca cctcatcaat
   116281 ctgccatttt tgctgtatct gattttctat attatcttaa ttcaatcgct tcagactttt
   116341 cgcaatctct gtaacactcc cccatttttt tgtaaataaa aagcgtttac gcaatgcaat
   116401 ccttttgtaa caacccaggt aaaatacctt attttcaagt tgttaaataa gtggttatct
   116461 acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca ttaactcttc
   116521 tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa gtgacgagtg
   116581 aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt tatttatcgt
   116641 taaatccaga taagtatcct aattatcgtc ttctgattca ctcagccaaa ttacaaaatg
   116701 aaataaagag ccactatacc aaagacgaaa ttcaaggatt gctaacatta acagaaaaca
   116761 cacgaaagtt aacgttgacg gagaaaccct ggggaacgtt tattcttgct tctactttcg
   116821 aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg gatagtttat
   116881 ggggctatat ggcattagta tcagaccagg gaaatagtgt agccgcaaaa aaggttctgc
   116941 tcaccctgtg ggattatatg tctacgcccg accagattaa gcggatgcag gacatcatta
   117001 gcaacccaaa acgactggat ggtattcccg ggcaaatgaa tgctgtgcat atttgacagc
   117061 aactcccccg tgatggcaga tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa
   117121 caaaatgatg cgttaggtct ttatcttgat ctccttatac aagcaatcaa tactggtact
   117181 attaacgctg aagattggca aaaaggtgac cgactaaaat cggttgcgct gctgattgct
   117241 tatctggata aagccaattt ctatgttatg gaagattccg gagcgtggga ggaagatgcc
   117301 cgattaaaca catcttccgt tgcactggtg acctctggac tggaacgttt gtcaaaccta
   117361 ctctcgaaga aagactctgt ttttgtctct gatttactac gcgaagctaa agtcaatgaa
   117421 ctggatgaaa ctctttcaac aacgaggttg aaccacctca tagataaagg ttatgaaaga
   117481 ataacgttac agcttgattt agggggagaa tccccaggtt atctggaaaa agataaacat
   117541 tacagagaag ccgatgcggc tttacttaat gttatttatc ctgcaaatct ttcaaagata
   117601 aacaccagaa gaaaagagca agttctaaaa atagttaaaa aactagcagg tccgtatggt
   117661 ataaaaagat acgagaagga taattatcag tcggcaaatt tttggttcaa tgatattaaa
   117721 accgataccg atcaaaattc gcatgcaaaa agagaaaaga gcttcattcc atcaacagaa
   117781 gcagaatggt tttttgactc atggtatgca aaatcagcag caattgttta taaagaatca
   117841 agaaaagaag agtatttgaa tgactcagta caatttatga atagatcatt ggcccaaata
   117901 acaggcgaga atatgattgg tgcaaatggc aggagcgtac cagaaatggc acttcccgaa
   117961 agctataact atatccacaa gtcgggaact ttgcatgaag cgccaagccc tatcattccg
   118021 ctaaattggt cgaaagcttc aatgactctc atgttgaaag agatgtccaa ccttatcaat
   118081 gatgagggaa ttaaataatt ttcataatga ggcggtcctg gattaaaagg accgctcaaa
   118141 gagaaatcga aaggggaggt aaaagtgatc aataaattac tgttagctta cctgataggt
   118201 ctggttgtga catcgacttt aatatttatc ttctcggaag aaaaagtaac atatcggttg
   118261 tttgctgcaa taattacagg tttaacctgg ccgcttagtc tcattccttc cattataagt
   118321 ctcatgattc gtaaatccga ctaacaagaa aattatatga tcgattaaga aaaatagaat
   118381 cgaacaagag aataaaatat atgcggggag taacaaaaca ctcgcagtgt gttaatgaag
   118441 aactaacaaa tatgatttgt attgaaaata gttaccaaac aagtgttata agtaatacga
   118501 ggtgtgttta agtaaaacat atcaacagtt acaaatctgt atatggacag gatgacgtac
   118561 tagtgtaagt ggctgatagc t
//
LOCUS       DAZGXS010000003        97124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGXS010000003 DAZGXS010000000
VERSION     DAZGXS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1062
                     /locus_tag="ACHKG5_000242"
     CDS             <1..1062
                     /locus_tag="ACHKG5_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0866427.1"
                     /translation="GLGKREITVKALSEKWLDLKKIEICANALNRYQSVIKNMLPMLG
                     EKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVVTVNYYMTTIAGMFQFAT
                     DNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQQTKNLWILAVYTGIRHG
                     ELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEM
                     TMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGPHYKVDSIRESWTSILKR
                     AGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIE
                     LLNKRLSESVPCMPHKKAG"
     gene            1083..1784
                     /gene="mlrA"
                     /locus_tag="ACHKG5_000243"
     CDS             1083..1784
                     /gene="mlrA"
                     /locus_tag="ACHKG5_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0866428.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            1844..1951
                     /gene="yohO"
                     /locus_tag="ACHKG5_000244"
     CDS             1844..1951
                     /gene="yohO"
                     /locus_tag="ACHKG5_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0866429.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1932..2663)
                     /gene="yehW"
                     /locus_tag="ACHKG5_000245"
     CDS             complement(1932..2663)
                     /gene="yehW"
                     /locus_tag="ACHKG5_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0866430.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2668..3594)
                     /gene="yehX"
                     /locus_tag="ACHKG5_000246"
     CDS             complement(2668..3594)
                     /gene="yehX"
                     /locus_tag="ACHKG5_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0866431.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(3587..4744)
                     /gene="yehY"
                     /locus_tag="ACHKG5_000247"
     CDS             complement(3587..4744)
                     /gene="yehY"
                     /locus_tag="ACHKG5_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0866432.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(4751..5668)
                     /gene="osmF"
                     /locus_tag="ACHKG5_000248"
     CDS             complement(4751..5668)
                     /gene="osmF"
                     /locus_tag="ACHKG5_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0866433.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5920..8217)
                     /gene="bglX"
                     /locus_tag="ACHKG5_000249"
     CDS             complement(5920..8217)
                     /gene="bglX"
                     /locus_tag="ACHKG5_000249"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0866434.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8413..10128
                     /gene="dld"
                     /locus_tag="ACHKG5_000250"
     CDS             8413..10128
                     /gene="dld"
                     /locus_tag="ACHKG5_000250"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0866435.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10166..11098)
                     /gene="pbpG"
                     /locus_tag="ACHKG5_000251"
     CDS             complement(10166..11098)
                     /gene="pbpG"
                     /locus_tag="ACHKG5_000251"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0866436.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11272..11859)
                     /gene="yohC"
                     /locus_tag="ACHKG5_000252"
     CDS             complement(11272..11859)
                     /gene="yohC"
                     /locus_tag="ACHKG5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0866437.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            12029..12607
                     /gene="yohD"
                     /locus_tag="ACHKG5_000253"
     CDS             12029..12607
                     /gene="yohD"
                     /locus_tag="ACHKG5_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0866438.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(12737..13498)
                     /gene="yohF"
                     /locus_tag="ACHKG5_000254"
     CDS             complement(12737..13498)
                     /gene="yohF"
                     /locus_tag="ACHKG5_000254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0866439.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13551..14987)
                     /gene="mdtQ"
                     /locus_tag="ACHKG5_000255"
     CDS             complement(13551..14987)
                     /gene="mdtQ"
                     /locus_tag="ACHKG5_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0866440.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15211..15294
                     /gene="yohP"
                     /locus_tag="ACHKG5_000256"
     CDS             15211..15294
                     /gene="yohP"
                     /locus_tag="ACHKG5_000256"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0866441.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15682..16632)
                     /gene="dusC"
                     /locus_tag="ACHKG5_000257"
     CDS             complement(15682..16632)
                     /gene="dusC"
                     /locus_tag="ACHKG5_000257"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0866442.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(16792..17985)
                     /locus_tag="ACHKG5_000258"
     CDS             complement(16792..17985)
                     /locus_tag="ACHKG5_000258"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP0866443.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(18000..18644)
                     /gene="maiA"
                     /locus_tag="ACHKG5_000259"
     CDS             complement(18000..18644)
                     /gene="maiA"
                     /locus_tag="ACHKG5_000259"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP0866444.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(18653..19354)
                     /locus_tag="ACHKG5_000260"
     CDS             complement(18653..19354)
                     /locus_tag="ACHKG5_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0866445.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(19369..20397)
                     /gene="gtdA"
                     /locus_tag="ACHKG5_000261"
     CDS             complement(19369..20397)
                     /gene="gtdA"
                     /locus_tag="ACHKG5_000261"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP0866446.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(20409..21767)
                     /locus_tag="ACHKG5_000262"
     CDS             complement(20409..21767)
                     /locus_tag="ACHKG5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0866447.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            21894..22802
                     /locus_tag="ACHKG5_000263"
     CDS             21894..22802
                     /locus_tag="ACHKG5_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0866448.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            22933..23331
                     /gene="yohJ"
                     /locus_tag="ACHKG5_000264"
     CDS             22933..23331
                     /gene="yohJ"
                     /locus_tag="ACHKG5_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0866449.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            23328..24023
                     /gene="yohK"
                     /locus_tag="ACHKG5_000265"
     CDS             23328..24023
                     /gene="yohK"
                     /locus_tag="ACHKG5_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0866450.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            24153..25037
                     /gene="cdd"
                     /locus_tag="ACHKG5_000266"
     CDS             24153..25037
                     /gene="cdd"
                     /locus_tag="ACHKG5_000266"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0866451.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            25187..25906
                     /gene="sanA"
                     /locus_tag="ACHKG5_000267"
     CDS             25187..25906
                     /gene="sanA"
                     /locus_tag="ACHKG5_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0866452.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            25909..26148
                     /gene="yeiS"
                     /locus_tag="ACHKG5_000268"
     CDS             25909..26148
                     /gene="yeiS"
                     /locus_tag="ACHKG5_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0866453.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            26342..27580
                     /gene="preT"
                     /locus_tag="ACHKG5_000269"
     CDS             26342..27580
                     /gene="preT"
                     /locus_tag="ACHKG5_000269"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0866454.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            27574..28809
                     /gene="preA"
                     /locus_tag="ACHKG5_000270"
     CDS             27574..28809
                     /gene="preA"
                     /locus_tag="ACHKG5_000270"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0866455.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29052..30062)
                     /gene="mglC"
                     /locus_tag="ACHKG5_000271"
     CDS             complement(29052..30062)
                     /gene="mglC"
                     /locus_tag="ACHKG5_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0866456.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(30078..31598)
                     /gene="mglA"
                     /locus_tag="ACHKG5_000272"
     CDS             complement(30078..31598)
                     /gene="mglA"
                     /locus_tag="ACHKG5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0866457.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(31659..32657)
                     /gene="mglB"
                     /locus_tag="ACHKG5_000273"
     CDS             complement(31659..32657)
                     /gene="mglB"
                     /locus_tag="ACHKG5_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0866458.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(32937..33977)
                     /gene="galS"
                     /locus_tag="ACHKG5_000274"
     CDS             complement(32937..33977)
                     /gene="galS"
                     /locus_tag="ACHKG5_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0866459.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(34119..35276)
                     /gene="yeiB"
                     /locus_tag="ACHKG5_000275"
     CDS             complement(34119..35276)
                     /gene="yeiB"
                     /locus_tag="ACHKG5_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0866460.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(35293..35961)
                     /gene="folE"
                     /locus_tag="ACHKG5_000276"
     CDS             complement(35293..35961)
                     /gene="folE"
                     /locus_tag="ACHKG5_000276"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0866461.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            36219..37055
                     /gene="yeiG"
                     /locus_tag="ACHKG5_000277"
     CDS             36219..37055
                     /gene="yeiG"
                     /locus_tag="ACHKG5_000277"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0866462.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(37087..39066)
                     /gene="cirA"
                     /locus_tag="ACHKG5_000278"
     CDS             complement(37087..39066)
                     /gene="cirA"
                     /locus_tag="ACHKG5_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP0866463.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(39358..40827)
                     /gene="lysP"
                     /locus_tag="ACHKG5_000279"
     CDS             complement(39358..40827)
                     /gene="lysP"
                     /locus_tag="ACHKG5_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0866464.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(41032..41913)
                     /gene="yieE"
                     /locus_tag="ACHKG5_000280"
     CDS             complement(41032..41913)
                     /gene="yieE"
                     /locus_tag="ACHKG5_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0866465.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            42012..43061
                     /gene="yeiH"
                     /locus_tag="ACHKG5_000281"
     CDS             42012..43061
                     /gene="yeiH"
                     /locus_tag="ACHKG5_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0866466.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            43135..43992
                     /gene="nfo"
                     /locus_tag="ACHKG5_000282"
     CDS             43135..43992
                     /gene="nfo"
                     /locus_tag="ACHKG5_000282"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0866467.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            43995..45083
                     /gene="yeiI"
                     /locus_tag="ACHKG5_000283"
     CDS             43995..45083
                     /gene="yeiI"
                     /locus_tag="ACHKG5_000283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0866468.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPPLSADNALSLVPMV"
     gene            complement(45139..46389)
                     /gene="nupX"
                     /locus_tag="ACHKG5_000284"
     CDS             complement(45139..46389)
                     /gene="nupX"
                     /locus_tag="ACHKG5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0866469.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46489..47430)
                     /gene="rihB"
                     /locus_tag="ACHKG5_000285"
     CDS             complement(46489..47430)
                     /gene="rihB"
                     /locus_tag="ACHKG5_000285"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0866470.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            47560..48258
                     /gene="yeiL"
                     /locus_tag="ACHKG5_000286"
     CDS             47560..48258
                     /gene="yeiL"
                     /locus_tag="ACHKG5_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0866471.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(48329..49579)
                     /locus_tag="ACHKG5_000287"
     CDS             complement(48329..49579)
                     /locus_tag="ACHKG5_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0866472.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49673..50611)
                     /gene="psuG"
                     /locus_tag="ACHKG5_000288"
     CDS             complement(49673..50611)
                     /gene="psuG"
                     /locus_tag="ACHKG5_000288"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0866473.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(50599..51540)
                     /gene="psuK"
                     /locus_tag="ACHKG5_000289"
     CDS             complement(50599..51540)
                     /gene="psuK"
                     /locus_tag="ACHKG5_000289"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0866474.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(51964..53655)
                     /gene="fruA"
                     /locus_tag="ACHKG5_000290"
     CDS             complement(51964..53655)
                     /gene="fruA"
                     /locus_tag="ACHKG5_000290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0866475.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(53672..54610)
                     /gene="fruK"
                     /locus_tag="ACHKG5_000291"
     CDS             complement(53672..54610)
                     /gene="fruK"
                     /locus_tag="ACHKG5_000291"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0866476.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(54610..55740)
                     /gene="fruB"
                     /locus_tag="ACHKG5_000292"
     CDS             complement(54610..55740)
                     /gene="fruB"
                     /locus_tag="ACHKG5_000292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0866477.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            56108..57289
                     /gene="setB"
                     /locus_tag="ACHKG5_000293"
     CDS             56108..57289
                     /gene="setB"
                     /locus_tag="ACHKG5_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0866478.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(57286..57540)
                     /gene="yeiW"
                     /locus_tag="ACHKG5_000294"
     CDS             complement(57286..57540)
                     /gene="yeiW"
                     /locus_tag="ACHKG5_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0866479.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            57695..58267
                     /gene="yeiP"
                     /locus_tag="ACHKG5_000295"
     CDS             57695..58267
                     /gene="yeiP"
                     /locus_tag="ACHKG5_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0866480.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            58490..59956
                     /gene="yeiQ"
                     /locus_tag="ACHKG5_000296"
     CDS             58490..59956
                     /gene="yeiQ"
                     /locus_tag="ACHKG5_000296"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0866481.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            60074..61060
                     /gene="yeiR"
                     /locus_tag="ACHKG5_000297"
     CDS             60074..61060
                     /gene="yeiR"
                     /locus_tag="ACHKG5_000297"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0866482.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            61099..61812
                     /gene="lpxT"
                     /locus_tag="ACHKG5_000298"
     CDS             61099..61812
                     /gene="lpxT"
                     /locus_tag="ACHKG5_000298"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP0866483.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            62225..62791
                     /gene="mepS"
                     /locus_tag="ACHKG5_000299"
     CDS             62225..62791
                     /gene="mepS"
                     /locus_tag="ACHKG5_000299"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0866484.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            62972..64528
                     /gene="pdeN"
                     /locus_tag="ACHKG5_000300"
     CDS             62972..64528
                     /gene="pdeN"
                     /locus_tag="ACHKG5_000300"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0866485.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            64610..66424
                     /gene="yejA"
                     /locus_tag="ACHKG5_000301"
     CDS             64610..66424
                     /gene="yejA"
                     /locus_tag="ACHKG5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP0866486.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            66425..67519
                     /gene="yejB"
                     /locus_tag="ACHKG5_000302"
     CDS             66425..67519
                     /gene="yejB"
                     /locus_tag="ACHKG5_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0866487.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            67519..68544
                     /gene="yejE"
                     /locus_tag="ACHKG5_000303"
     CDS             67519..68544
                     /gene="yejE"
                     /locus_tag="ACHKG5_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0866488.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            68546..70135
                     /gene="yejF"
                     /locus_tag="ACHKG5_000304"
     CDS             68546..70135
                     /gene="yejF"
                     /locus_tag="ACHKG5_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0866489.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(70139..70483)
                     /gene="yejG"
                     /locus_tag="ACHKG5_000305"
     CDS             complement(70139..70483)
                     /gene="yejG"
                     /locus_tag="ACHKG5_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0866490.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(70816..72006)
                     /gene="bcr"
                     /locus_tag="ACHKG5_000306"
     CDS             complement(70816..72006)
                     /gene="bcr"
                     /locus_tag="ACHKG5_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0866491.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(72034..72729)
                     /gene="rsuA"
                     /locus_tag="ACHKG5_000307"
     CDS             complement(72034..72729)
                     /gene="rsuA"
                     /locus_tag="ACHKG5_000307"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0866492.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            72878..74638
                     /gene="radD"
                     /locus_tag="ACHKG5_000308"
     CDS             72878..74638
                     /gene="radD"
                     /locus_tag="ACHKG5_000308"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0866493.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            74763..75047
                     /gene="rplY"
                     /locus_tag="ACHKG5_000309"
     CDS             74763..75047
                     /gene="rplY"
                     /locus_tag="ACHKG5_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0866494.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(75186..76193)
                     /gene="yejK"
                     /locus_tag="ACHKG5_000310"
     CDS             complement(75186..76193)
                     /gene="yejK"
                     /locus_tag="ACHKG5_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0866495.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            76375..76602
                     /gene="yejL"
                     /locus_tag="ACHKG5_000311"
     CDS             76375..76602
                     /gene="yejL"
                     /locus_tag="ACHKG5_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0866496.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            76622..78382
                     /gene="yejM"
                     /locus_tag="ACHKG5_000312"
     CDS             76622..78382
                     /gene="yejM"
                     /locus_tag="ACHKG5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0866497.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            78457..78533
                     /locus_tag="ACHKG5_000313"
     tRNA            78457..78533
                     /locus_tag="ACHKG5_000313"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78491..78493,aa:Pro,seq:ggg)
     gene            complement(78634..81225)
                     /gene="yejO"
                     /locus_tag="ACHKG5_000314"
     CDS             complement(78634..81225)
                     /gene="yejO"
                     /locus_tag="ACHKG5_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFP0866498.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            81545..82192
                     /gene="narP"
                     /locus_tag="ACHKG5_000315"
     CDS             81545..82192
                     /gene="narP"
                     /locus_tag="ACHKG5_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0866499.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(82227..83279)
                     /gene="ccmH"
                     /locus_tag="ACHKG5_000316"
     CDS             complement(82227..83279)
                     /gene="ccmH"
                     /locus_tag="ACHKG5_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0866500.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(83276..83833)
                     /gene="dsbE"
                     /locus_tag="ACHKG5_000317"
     CDS             complement(83276..83833)
                     /gene="dsbE"
                     /locus_tag="ACHKG5_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0866501.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(83830..85773)
                     /gene="ccmF"
                     /locus_tag="ACHKG5_000318"
     CDS             complement(83830..85773)
                     /gene="ccmF"
                     /locus_tag="ACHKG5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0866502.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(85770..86249)
                     /gene="ccmE"
                     /locus_tag="ACHKG5_000319"
     CDS             complement(85770..86249)
                     /gene="ccmE"
                     /locus_tag="ACHKG5_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0866503.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(86246..86455)
                     /gene="ccmD"
                     /locus_tag="ACHKG5_000320"
     CDS             complement(86246..86455)
                     /gene="ccmD"
                     /locus_tag="ACHKG5_000320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0866504.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(86452..87189)
                     /gene="ccmC"
                     /locus_tag="ACHKG5_000321"
     CDS             complement(86452..87189)
                     /gene="ccmC"
                     /locus_tag="ACHKG5_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0866505.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(87231..87893)
                     /gene="ccmB"
                     /locus_tag="ACHKG5_000322"
     CDS             complement(87231..87893)
                     /gene="ccmB"
                     /locus_tag="ACHKG5_000322"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0866506.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(87890..88513)
                     /gene="ccmA"
                     /locus_tag="ACHKG5_000323"
     CDS             complement(87890..88513)
                     /gene="ccmA"
                     /locus_tag="ACHKG5_000323"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0866507.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(88526..89128)
                     /gene="napC"
                     /locus_tag="ACHKG5_000324"
     CDS             complement(88526..89128)
                     /gene="napC"
                     /locus_tag="ACHKG5_000324"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0866508.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(89138..89587)
                     /gene="napB"
                     /locus_tag="ACHKG5_000325"
     CDS             complement(89138..89587)
                     /gene="napB"
                     /locus_tag="ACHKG5_000325"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0866509.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(89584..90447)
                     /gene="napH"
                     /locus_tag="ACHKG5_000326"
     CDS             complement(89584..90447)
                     /gene="napH"
                     /locus_tag="ACHKG5_000326"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0866510.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(90434..91129)
                     /gene="napG"
                     /locus_tag="ACHKG5_000327"
     CDS             complement(90434..91129)
                     /gene="napG"
                     /locus_tag="ACHKG5_000327"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0866511.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(91136..93622)
                     /gene="napA"
                     /locus_tag="ACHKG5_000328"
     CDS             complement(91136..93622)
                     /gene="napA"
                     /locus_tag="ACHKG5_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP0866512.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(93619..93882)
                     /gene="napD"
                     /locus_tag="ACHKG5_000329"
     CDS             complement(93619..93882)
                     /gene="napD"
                     /locus_tag="ACHKG5_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0866513.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(93872..94366)
                     /gene="napF"
                     /locus_tag="ACHKG5_000330"
     CDS             complement(93872..94366)
                     /gene="napF"
                     /locus_tag="ACHKG5_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0866514.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            94475..94639
                     /gene="yojO"
                     /locus_tag="ACHKG5_000331"
     CDS             94475..94639
                     /gene="yojO"
                     /locus_tag="ACHKG5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP0866515.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            94773..95261
                     /gene="eco"
                     /locus_tag="ACHKG5_000332"
     CDS             94773..95261
                     /gene="eco"
                     /locus_tag="ACHKG5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0866516.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(95411..97057)
                     /gene="mqo"
                     /locus_tag="ACHKG5_000333"
     CDS             complement(95411..97057)
                     /gene="mqo"
                     /locus_tag="ACHKG5_000333"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0866517.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        23668 a        26249 c        24073 g        23134 t
ORIGIN      
        1 ggtctgggaa aaagagagat aaccgttaag gcactttcgg aaaaatggtt ggaccttaag
       61 aaaattgaga tttgtgcgaa tgcacttaac cgttaccagt cagtaattaa aaacatgtta
      121 ccaatgttag gtgaaaaaaa actggtttca tccataacaa aagaggattt acttttcgta
      181 aggagagatt tgttgaccgg ttaccaaaag ctttctaatg gaaagacttc ttccataaaa
      241 gggcgctcag tggtcacggt aaactactat atgacaacca tagctggaat gtttcaattt
      301 gcaacagata atggttatac ctcaggaaac ccatttaacg gtctggctcc cttaaaaaag
      361 tccaaggtaa aaccagatcc tctcacccgt gacgaattta ttcgttttat tgaggcttgc
      421 cgtcatcaac aaacaaaaaa cctgtggatt ctcgctgtat acacgggtat tcgtcacggg
      481 gagttggtat cgctggcatg ggaagatata gaccttaaag caaggactat aaccatccgt
      541 agaaattata caaaacttgg cgaattcact ccaccaaaaa ccgatgcagg caccggaagg
      601 acaattcatc tggttcaacc agctattgat gctcttaaaa gccaggcgga aatgaccatg
      661 cttggaaagc aacattctgt agaggtgaag cagagggaat atgggagaac tgctgtgcat
      721 aaatgcactt ttgtttttag tcctcaggta acaaaacagc agcagttgtc cggacctcac
      781 tacaaggttg actccatcag ggagtcatgg acaagtatct taaaacgcgc aggtctgaga
      841 cacagaaaat cgtaccaatc caggcatact tatgcatgct ggtcacttgc cgcaggagct
      901 aatcctagtt ttatcgcaag ccagatgggc cacacaaacg cacaaatggt attcaatgtt
      961 tacggagcat ggatgaaaga caacaatcac gaacagatag aactccttaa caaaagacta
     1021 tctgaaagtg tcccatgtat gccccataag aaagcagggt aaaataaaaa cttgcaaaat
     1081 caattagttt acccttaatc cctgtcacgt tacgcgcgtg gcagaggcgt tacggattgc
     1141 tgaaaccgca acggacagac ggcggtcatc ggctgttcaa cgatgccgat attgaccgga
     1201 tccgcgagat caaacgctgg atcgacaacg gcgtgcaggt cagcaaagtt aaaatgctgc
     1261 tcagtaatga aaatgttgat gtgcagaacg gctggcgcga tcagcaagaa acattactga
     1321 cctacctgca aagcggcaat ctgcatagcc tgcgaacgtg gatcaaagag cgcggtcagg
     1381 attaccccgc ccagacgctc accacacatc tgtttattcc tctgcgccga cggcttcagt
     1441 accaacaacc gactctccag gcgctgctgg cgatcctcga cggcgtactg atcaactaca
     1501 tcgccatttg tctggcttcg gcacgtaaaa aacagggtaa agatgcgctg gtggttggct
     1561 ggaatattca ggataccacc cgtctgtggc tggagggctg gattgccagt caacaaggat
     1621 ggcgcattga tgtcctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc
     1681 gtacattgct ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca
     1741 gttggcaaga acaaggccat gatattttcc cactcggcat ttaatgattc gttaacaaat
     1801 gcgctttact gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat
     1861 tggggtcatc gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc
     1921 aggttatacc tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg
     1981 tctgcgatga tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta
     2041 aatccgctaa gcccgatgat gatgggcgta cccagcgtgc tggcccctac cgttgaggcg
     2101 atcgtcgccg taccaatgtt gataatcacc gaggttcgca cgcccgccag aatcaccgga
     2161 gccgccagcg gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc
     2221 gcaacttctg tcacgctggc atcaatcgct cccagcccgg caagtgtcgc ctgcaggacg
     2281 ggcagcacac cataaaggat caaggcgata atcgctggtt gcagaccaaa gcctatcacc
     2341 ggaacagcaa tcgccagcac tgcgacaggc ggaaaagtct gcccaacggc ggcaatagtt
     2401 tccaccagtg ggcgaaattc cgcgccccac gggcgagtga ctgcaattcc ggcaccagtg
     2461 ccaatgatca ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaaa
     2521 gctgcaaaac tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac
     2581 agcggctggc tgtaaggcag ccagaaaatc agtgccacaa acaaagcaat gagccagaac
     2641 agcggatcgc gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag
     2701 cgtgccgcta ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac
     2761 aaacagggag agcgcatcgc gcagcgtcat ctcttctgcc agtgcctcac cttctgcccg
     2821 ttcttcgcga cgcacgtaat ccgccacact acgtaacgaa agcaggcgca cacccagttc
     2881 actacgtcca aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg
     2941 attcccctgc tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag
     3001 cgcctcatca atatcatgcg tgaccagcac aatggtacgc cccagcaaac ggtgaatgcg
     3061 cgtcatctct tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc
     3121 catcagtaag acttgcggat cggcagccag tgcgcgcgct actcccacac gttgctgctg
     3181 accaccggaa agctgatgtg gataacgctc acgtaaattt gactccagcc ccagtagcgc
     3241 cattaattcg tcgatacgat cgtcaatcct cgcccgcgac catttttgta attgcggcac
     3301 ggtggcaatg ttttgtgcca cgctccagtg gggaaacagg ccaatagatt gaatggcata
     3361 gcccatacgg cggcgcaact ccagtactgg cagcgagcga atttcttctc cagcaaagcg
     3421 gagctctccg ctgtcatgtt ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc
     3481 ggagccagat gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc
     3541 gttaacggct ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt
     3601 tcaccttcaa cagtgcgatt agtaaatcga acagcgcatc gatcagcacc gccagaacaa
     3661 ttaccgggat cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa
     3721 ccagcgcacc aaaaccgcct gcgccgatta acgccgcaat caccgccatg cctacagttt
     3781 gcaccatcac cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat
     3841 gcaggaatcg ctgcgccccg ctcatgccca tcgctctggc gctctccagc acatcgcgcg
     3901 ggatctggtt caagccgact accacgccgc gcaccagcgg tagcaaggca tagagcacca
     3961 gcgcaatcag tgcgggtgtc attccggttc ctgctatgcc aagcttcccc agccacggaa
     4021 aggccgtaac cagtccggca agcggcgcaa tcagcaggcc aaagagcgcc acggaaggca
     4081 cggtctgaat gacattgagc agagaaaaaa tcgccccctg ccgcgcggtg gaaaagtagc
     4141 accagatgcc caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg
     4201 tcagatgttg tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcatta
     4261 gtgagagatc gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca
     4321 tatgcaacaa ccagcgccac agcggatgcg tggagattcg gcggatggca tcgctacagg
     4381 ccagcaatgc cagcgccgaa gccaaccaga aaccactgcc gaggctggta cgcgccagcg
     4441 cactgccatt ttgcgccagt tgggtcgccg cctttccagc tccccacacc agcaatacga
     4501 agacgaattg cgccagaatg agtgcaaaaa tgctgccttt tttagcggga ataaaacagg
     4561 ctgtcagcca ggcgcaacca acgcccacca gcatccagat cgtttgcggc cacagttgcc
     4621 agagatgacg cccttcaccc gagactaaac gattaggcgc gtagctgata aacggcagcg
     4681 ctgctgcgat tgccgtcagc aacagcagca gcgccagaac aggattaata cgtagataag
     4741 tcacgggaaa ttacttcgtc cacccttttt gtttcaagta gtcggcagcc acttttttgg
     4801 catccagtcc ttccactgca atgctggcat tcagttgctg caatgttttt gcatcgaggc
     4861 tggcgaagac tggctgtagc cactgtgcca tttgcggata ctctttcagc accgactcac
     4921 gcaccactgg tgcaggcgcg tagataggtt gcacgccttg cggatcgctt aaggtttgca
     4981 gcccctgcgc cgcgaccgga ccgtcagtgc cgtaagccat tgcggcatta acgccagaag
     5041 tttgctgggc agcggcttta atcgttaccg ccgtgtcgcc gccagccagt gacagtaact
     5101 gatcctgatc gagtttaaag tcgtaggctt tttcaaacgc gggtaacgca tcggcgcgtt
     5161 cgataaactc agccgaggct gccagtttga aggtgccgcc ctctttcaga taacgcctca
     5221 ggtcagcaag cgaagtgagt ttgtttttct ctgccacatc ctgacgcacg gcgatggtcc
     5281 aggtgttatt tgcaggtgcg ggcgtcagcc agattaactt gttttgctct gcatcgagtt
     5341 ttttgacttt ctcgtaacct tgctgcgcgt ttttccacgc tgcatcgttt tcatctttaa
     5401 agaaaaacgc gccattgccg gtatattccg gatagatatc cagttcaccg gaagtaatcg
     5461 ccccgcgcac cacaggagtc gttccaagtt gcactttatt gaccgttggt actccgtggc
     5521 tttcgagtac ttgcaaaatg atattgccga gcagcgcgcc ttcggtatcg atttttgaac
     5581 cgactttaac gggggaagcc gcgtgcagcg gcaggctcac ggctgccaac aaaaccagtg
     5641 aacctgccca gacctttgag agtggcatga tgctttcctc attcttttac tgttgttttc
     5701 agcgaattaa gagaaaagca tagttgataa tggcgggatt agcctgagcg gacggattca
     5761 gttgcagaat cagataaata cttaagaggc atattcggtc cggcgtttcc tgccggatgc
     5821 ggcgcgagcg ccttaaccgg cctacaaagg gcgaaaattc agcaagttaa gatatgaaca
     5881 tcaggcaaag ggcgcaaacg tcgcgccctt gctaaagcat tacagcaact caaactcgcc
     5941 tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag gctcggcgtc
     6001 atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga agctgacggt
     6061 ctgggtttcg cccggcttca gggtgatttt ctcaaagcct ttcagctgct tcactggacg
     6121 gctcatggaa gccgtcacat cctgcaagta catctgcact accgtggccc cttcgcgttt
     6181 accggtgttc gtcacctgca cgctggccgt cactttgccg tcacgcttca tggtcggcgc
     6241 agaaagtttc acatcagaga cggtgaaagt ggtatagctc agaccatagc cgaacggata
     6301 aagcgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct tgtcggcatt
     6361 atacggacga ccggtgttca gatggctgta gtacaccggg atctgcccga cagaacgcgg
     6421 gaaggacatc ggcagcttac cggacgggtt gtaatcgcca aacaacacat cggcaattgc
     6481 attaccgcct tcagtcccgg caaaccaggt ttccagaatc gcatcagcct gctgatcttc
     6541 tttcaccagc gccagcggac gcccgttcat cagcaccagc accagcggtt tgccggtggc
     6601 tttcagggcg gcaatcaaat cacgctggct ttgcagaata gtaatatcgg tacggctgga
     6661 ggcctcgtgc gccatcccct gtgcttcacc gactacagcc accaccacat cagattgttt
     6721 cgccgtctgc accgcttcat caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc
     6781 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt
     6841 ggcatacagc actttaccgt tttcaccgac ggcattttta atcccggtca gcacggtcac
     6901 ggattgatcg gcaacacctg ccgccgacca gctgcccatc acgtcacgtt tactgtccgc
     6961 cagtggccca accaccgcaa tggtggctga ttttttcagc ggtaacgttt cgagacggtt
     7021 tttcagcaac accaggcttt cacgcgccac ttcacgcgct tctttgcggt gcaggcggct
     7081 ttcggcattg gtatccaccg ggtcagactc tttcggaccg agatggctgt atgggtcgtt
     7141 aaacaacccc atatcatatt taacgttcag tacatggcgg gcagcatcgt ccagctctgc
     7201 catcgtcact ttaccggatt tgatcaaccc aggcagatac ttcgagtagt actcgtcgct
     7261 catgctcatg ttgattccgg atttcagcgc cacgcgcact gcatcttccg gatctgccgc
     7321 cgtgccatgt ttaatcagct ctttgattgc accgtgatcg gaaacggtga tgcctttaaa
     7381 gccccactgg tcgcgcagaa catctttcag cagccaggag tcggaggtgg ctggcgtgcc
     7441 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta
     7501 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     7561 accgccttct accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtaacg
     7621 atctgccggg cttttaccct gcatcgcttc caccatggtt ttacccatta ttgaggtgag
     7681 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     7741 gaccatcggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     7801 tccgaccgtt tttactgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     7861 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     7921 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
     7981 agccccaacc tgaccgtctt tgatcatctc gcggatcgcc tctttcgggt tatccgggcc
     8041 gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct taagcagttc
     8101 ggtgacgaac gcatcccgcg cttcaggcgt taatggatgg ttaccgaata aatcatccgc
     8161 cagtgctggc tgcagggcca gactcaccgc gattcctact gaacatagcc atttcatatg
     8221 gatttttctt cctttgttgc ccgacgtggc agcgaaaatg gtgcaaaaac cgtagtttgc
     8281 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
     8341 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatgttttt tcgccaccac
     8401 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
     8461 gcccgtctgg tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc
     8521 aagggcttcc gttctggtca tggcgacgcg ctggctgtcg ttttccctgg ctcactactg
     8581 gaattgtggc gggtgctgaa agcctgcgtc accgccgaca aaattattct gatgcaggct
     8641 gccaatacag gcctgaccga aggatcgacg ccaaacggta acgactatga tcgcgatatc
     8701 gtgatcatca gcaccctacg tctcgacaag ctgcacgttc tcggcaaggg cgaacaggtg
     8761 ctggcctatc cgggcaccac gctttattcc ctggaaaaag ccctcaaacc gctgggacgc
     8821 gaaccgcact cggtgattgg ttcatcgtgc attggcgcat cggttatcgg cggtatctgt
     8881 aataactctg gtggttcgct ggtgcagcgt ggcccggcgt ataccgaaat gtcgttattc
     8941 gcgcgtataa atgaagatgg caaactgacg ctggtgaacc atctggggat tgatctgggc
     9001 gaaacgccgg agcagatcct tagcaagctg gatgacgatc gcatcaaaga tgacgatgtg
     9061 cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
     9121 gccgacacgc ccgcacgtta taacgccgat ccggatcggt tatttgaatc ttctggttgc
     9181 gccgggaagc tggccgtctt tgcagtacgt cttgatacct tcgaagcgga aaaaaatcag
     9241 caggtgtttt atatcggcac caaccagccg gaagtgctga ccgaaatccg ccgtcatatt
     9301 ctggctaact tcgaaaatct gccggttgcc ggggaatata tgcaccggga tatctacgat
     9361 attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
     9421 atgccgttct tctttaatct caagggacgc accgatgcga tgctggagaa agtgaaattc
     9481 ttccgtccgc attttactga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
     9541 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg
     9601 gcgggcgatg gcgtgggcga agccaaatcg tggctggtgg attatttcaa acaggccgaa
     9661 ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc
     9721 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
     9781 gcgctggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
     9841 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
     9901 caggattaca tcgttaaaaa aggcgtggat gtgcatgcgt tgaaagaaca gatgctggag
     9961 ctgctacagc agcgcggcgc gcaataccct gccgaacata acgtcggtca tttgtataaa
    10021 gcaccggaga cattgcagaa gttttatcgc gagaacgatc cgaccaacag tatgaatccg
    10081 gggatcggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa attacggatg
    10141 gcagagtatc gccatccgaa ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    10201 tttgggcggc tttttgtttt ttatagctta acgctgctgc cggaacaggc atcactttac
    10261 cggtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtgtat ttgccaaacg
    10321 catccatcac taccagcgcc accggtttat tattgataac cgtacgcatc accagacaat
    10381 ggcccgccgc attggtaaag ccggttttgg ttagctggat attccagtta tcgcgataca
    10441 ccagatgatt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    10501 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    10561 tggttaagtc acgggcggtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    10621 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    10681 aaccaccggg ataatggtgc gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    10741 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    10801 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    10861 catccagcac gaccatcgca gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    10921 ccgggtggtt cgaatagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    10981 aggcaatttc cggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    11041 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    11101 tgagcattca gatagtggtt cacgcgcacg ggttgcgcac cgccggagta aggatttact
    11161 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    11221 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    11281 acgataaccg tagccccata gtataacggt aagggcgagc agcacttcca gtaccagcac
    11341 gccaatcgcc agcgtcgaac tggagaagct aaggccttcc tctttgttga tattcaggaa
    11401 gctcggaata ccaaggtaaa gtaggtaacc ggtgtaaaac agtgccaccg tgccgaccag
    11461 cgcacacaac cagaccagcg gataaagcgc caccagacca cttaaaaaca gcggagttgc
    11521 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    11581 catccaccag atgacccgcc ccatcaccgc gaccccagcg agcataacgc cataaaacag
    11641 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    11701 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    11761 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    11821 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    11881 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gcccatgtaa
    11941 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga ttcacagggt gtatgcttac
    12001 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    12061 ttatgccgcg ctggttatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    12121 ccttgcggca catcaggggc tattaaagtt cccgctggtg gtactttctg tagcgcttgg
    12181 cggcatgatt ggcgaccagg tgctctatct gtgcgggcgg cggtttggcg gtaagctgtt
    12241 acgccgtttc tctaaacatc aggataaaat tgagcgggcg cagaaactga tccaacgcca
    12301 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    12361 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgcttaata ttctcggcgc
    12421 atttgcctgg gcgttgattt ttaccactat cggttacgct ggtggtcagg tgattgcgcc
    12481 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    12541 tctggtggta ggcgtgcgct ggtggctgaa acgacgcggg aagaaaaagc cggataatca
    12601 ggcgtaaaac cattgccctg gataaggcgt gcgcgccgca tccgacatcc ggttcaccat
    12661 gcctgatgcg gcgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaatatc
    12721 cacacacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    12781 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc cgaacaaagc cacgccacca
    12841 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    12901 gcttcacgtc gctatcatcc atgccgttca ttggcgtagc gatcgcccca ggtgcgactg
    12961 cgttcaccaa aatcttatgc ctgaccaact ccagcgccat tgctttggtt aacccgccga
    13021 gcgcatgttt agcggctgtg taggcgctgg catccggcag cggcgtatgt tcatgtaccg
    13081 acgtaatgtt gatgatgcga ccaccctgtc cttgtttcac catctggcgg gcagcaattt
    13141 gcgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    13201 ccatatcaag aaacggcact ttggtcattg cgcccgcatt attcaccagc acatcaatac
    13261 gccccagccg ttgaataagt ttctccagcg cctgcgcacc ttctggcaga ttgccgagat
    13321 ccagttgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    13381 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccttgctgc gccagtaata
    13441 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgctacc tgtgccatcg
    13501 agttctccac ttaacgctga ataaacgtta agtatagaag tcacatatca tcagcgtttg
    13561 taacccccgc ccaacgcacc agtgagttga atggaggcat ccagccactg cccttgcaat
    13621 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    13681 gcaatgatgc ccgcgttaaa gcgggcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    13741 tcaatttgcg cctgatgctg gtttttctcc gccagtgtct gaacctgact ggctgcccgc
    13801 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    13861 tcggcttttg cgatatcgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    13921 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    13981 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    14041 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    14101 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgccacc
    14161 ggtttaagcg tcaatgactt agtctggtga tttgtgagtg caattaaccg tgcctcaata
    14221 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    14281 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    14341 ataatggtgt tctgctcttt ttcgatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    14401 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    14461 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    14521 cagatatcga gatgccagcc cgccgttaag ccaaaagttc cgttggtgta ccacggaccg
    14581 gtcgtacctg cggccggatc gttcagagca aacggcccca ttagcccttc tgccgacatt
    14641 ttttgccgct ccatatccgc agaaaagtcg atctgcggac catcctgagt agcaactgcc
    14701 ttcgcctggg cttcagctaa ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    14761 tgtagtgcat tgttaattaa ggaagtgagt tgattatcgt gatactccag ccaccattgg
    14821 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    14881 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    14941 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    15001 ggtaagaaaa ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    15061 caatacatca gtagcaataa aacgacatat ccagaaaaat atacactaag tgaatgatat
    15121 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    15181 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    15241 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaaatca
    15301 gatatgacat ataagttgtc aggtcaggaa ctagatattt tgtgtacttg atttgcaaac
    15361 agctccggtg ttggtggcgt caaaatcgct gaagcgttcc ccgaaggccg gggcagccca
    15421 catggatgtg ggctgagggc gcgtcttaca aggacgtttc ctcgcgcccg acccggtagc
    15481 cgaaggggat aagctgaagg taccgcgcag cggcgatttt gttcgccaga gcccgggggt
    15541 gcagggggag gaggcgattg tcctccccct gcgcgctcct tgcgccagtg gcaatatgtg
    15601 gcttagctca tgaaaggagc gcaacaagat gatgaagctg aataagcgat tcattgtttc
    15661 tgataaactg cgattccact attatcgcag cttaccgata tcaattgcct gaatagccct
    15721 tgcaatatca ggggaattat tcaacacacg aacatgctga aataattctg ttgcttcatc
    15781 gtattcttta cgcaaataac tcaaccactg tttaatccgc gctacgtgat ataacccggt
    15841 atcgccctgc ttttccagac gggtatattt ttgcagcaaa gcaaccacct ccggccacgg
    15901 cattcgcggt tcgttatatt ttactacccg gctcaggttg ggaatattga gcgccccgcg
    15961 accaatcatc actgagtcgc agccgctgat cgccatgcat tcttgcgcgc tctgccagtc
    16021 ccagatttcg ccgttggcaa tcaccggaat attcagccgc tggcgaatct cgccaatcgc
    16081 ctgccagtca atatgctccg cgcggtaacc ctgctctttt gtccgtccat gcaccaccag
    16141 ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca aatttcttct caccgctgtc
    16201 ccagcccaga cgcactttca cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc
    16261 ttttgcaccc tggtagatga gttcaggatc tttgagtaac gtcgccccac caccgctacc
    16321 gttaaccgtt ttcgacgggc agccgcaatt gagatccacg ccccaggaac ctaattccac
    16381 cgcacgggcg gcgttctctg ccagccattg tgggaactga cccaacaact gcacgcgcac
    16441 cagcgtaccg gatggtgtcc ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac
    16501 tttcaccggc agcagttgat ccaccacgcg gacgaactcg gtgatgcaca gatcgtagtc
    16561 gttaacttcg gtcagcaatt cacgcaccag agagtcaagc actccctcca tcggagccag
    16621 taacacacgc atatcatcac ccgcaaaaaa atgaggcgct atgttagcgc ctcctgtgtt
    16681 tgcagacaaa ctgacttact gattaattcg ggcaagattt ttaccctcac cctaaccctc
    16741 tcccgaaggg gcgaggggac cgcccgtgcg cctgattggg tgaagaagtg cttagcgtgg
    16801 ttgcaaacag ttatcttctt tccagccgta caaccactcc atgccgcgat aaaattctgc
    16861 ctgcgatttc cctttccaca gcagattacg tacctggcgt tctacccctg cggcgtggta
    16921 aacccgcccc atttcacggg tcgaccagac gatgcgcgcg gtgcgcggaa ttcgtaccga
    16981 ctcatacagc gcaaaggctt gcgccgcatc cccctcacac tgcgccagcg ccttacccag
    17041 cgttactgca tcttccagcg ccatacaggc tccctgcgcc atatattgcg ccaccggatg
    17101 ggccgaatcg cccacaagcg ttatgcgctt cgttccccac ttcgccaccg gttcacggtc
    17161 agcggttgac cagcgtcgcc atgaagtcgg tttatccaac atctggcgcg gacgggggtg
    17221 aatcccttca aagtaagaga gcacttcctc tttactacca tccttcacgc cccactcttc
    17281 ctgctggcgg ctgtggaatg ttaccaccag gttatactgc ttgccgccgc gcaaagggta
    17341 atgcaccaag tgacaatgcg gccccgccca cagtaccggt gcgttaatgc gcaaatcttc
    17401 cggcatatcg gcacgatcga taaccgcacg gtagacaaca tgtccggtga cacgcggcgc
    17461 atcgcccaat aagctctggc gcaccactga cttcacgccg tcacagccaa tcagaatatc
    17521 gccggtccag ctattgccgc gatcgtcaaa cacggtgaca tcgtcttccg tctcgcgaat
    17581 atccacgacg ttggtggagg tgtgatattc cacgtccgga tgcgtcagta ccgcttccca
    17641 caccgaggcg tgaatatcaa cccggtggat caccgcgtaa gggccgccga aatggtcacg
    17701 aaaggcctgc ccggtttcaa tgcacaccac ttcttcggcg ttcaccgcat ccatcatggt
    17761 aatgtgatcg gtaaacaccg cgcgctggcg ggcaacttcg ccgacgccga ggctgtccag
    17821 cgccgaaaac gcatttggcc ccaactggat gcccgcgcca atctcaccga tctcatgcgc
    17881 cttttccagc agcaccactt tgatccccag gcgggccagt gacagcgcag ttgccgcacc
    17941 gccgatcccg cctcccacaa ttactgcgcg tgtcactttc gtcattatag ttctcctggt
    18001 caggctgaaa ttttatcttg ttgattttct ggcgctgcgg caatgaacgc tggcagctgt
    18061 gtgcaggcgt cgtacaccgc tttgcagcgt ggatacccgc tcaaatcgca gttcatgcgt
    18121 agcgcgttcg cccactgcgg gacaaggcag caatctgcca gcgtcggcgt ctcgcccaca
    18181 caaaattgcc ccgactggtt ctggcgcaac agttgttcca ccgcgcttaa cccttgctgg
    18241 atccagtgcg cataccaacg ttttttttct tcttcactga cgttcagttc ttcggtcagg
    18301 tagcgcagca cgcgcaagtt attcaatgga tgaatatcac aagcgatggc atagacaatt
    18361 tccagcacct tgttgcgggc cggctcttcc tgcggcacca gcggaacttg cgggtagtgc
    18421 ctctccagcc agtcaataat cgccagcgat tgtccgagtg attcgccctc gtcagtcagc
    18481 agcgtcggca ccagcccgac cggattcatc cgccgatagg ccagttcatt ttgctgacca
    18541 atacggatat tcaccccttc ggtctggtaa ttaatgcctt tcagcgccag cgcaatgcgt
    18601 acccggtacg acgctgaact attaaaaaaa ctgtacagct tcatcatgca cctcagacaa
    18661 tcttcaccgc gataggcgtt aagccctcga cgttgcctgt gatcacatca cctttcacta
    18721 ccgggccaac cccttccggc gtaccggtga agatcagatc gcccggttgc agctcaaaga
    18781 agcctgacag atagcagatg gtttcattca ccgaccagat gagatggcga atatcgctgc
    18841 gctgatgatc ttcgccgtta acctgtaacc agattggcgc attatcaata ccgccgatat
    18901 cgcgggcttt gtgcagcggc gcgatgggcg ctgagagatc aaacgcttta ccgatttccc
    18961 acgggcggcc catctggcgc atttccattt gacggtcacg ccgagtcata tcaagacccg
    19021 tggcataacc ccagacatat tcatgggcgt gttccagcgg gatatcgctg ccttttttac
    19081 caatcgccac caccagttca atttcgtaat ggtagttatc ggtctgtgcc ggatacggca
    19141 gcgccagcgt ttcaccagcg gcaaccggaa caacggcatc cgccggtttg cagaagaaga
    19201 atggcggttc acgatccgga tcaaagccca tttcgcgggc gtgagcggca tagttacgtc
    19261 cgacgcagta aacgcggcgc acggggaatt gttcatcact gccaacaacc ggtacggtaa
    19321 cgggcgcttg cggttcgaat acgtattgag tcatttttct ctcctaaatt aataacgcgc
    19381 ttcgcggaac agccccaggg cttcttgtac cggtctgtcc gaaaaactaa ataaaacgga
    19441 atcttgcgtg gtctggaacg acacgccatg ccaggtcggc accacgaaga tatcttttgc
    19501 actaaagctg aaggtttcat tgccgatgat gacctgcccg ctaccttcca ccacatggta
    19561 gatggtgctg tcagtggtac gcgcaacgcg tgaggcaaac cctttcggca gcagttgcag
    19621 gaaagcgccc atcgacggca tcggatagcc gccggtgacc gggttgacgt agcgcatttt
    19681 gtagccatcc cactcgtcgg catcgcccag tcgggttaaa tcgtgcagca cttcgcggct
    19741 gcggtcataa cgatagttaa agatcggcga ggagttcccc gtctgatgcc gcagcggcaa
    19801 catattggca gcgtaacgcg gcagataatc tccctcttta cgcgttaccg gttgttgctc
    19861 ttccggataa tcttcggcaa aaccgcagcc cagaatattc actaatggca gatccagccc
    19921 atcgagccag ataaccggtt cgtcgccagg gttaccgtga tcgtgccagc gccactgcgg
    19981 ggtcaggata aaatcgcctt cattcattgg cgtgcgttcg ccatcaacgg cggtaaatgc
    20041 ccctttgcct tcaacaataa aacgcagcgc cgactgatta tgacgatgac tcggtgccac
    20101 ttcgcccggc atgatcagtt gcaaaccggc atataaagtc gcggtaatgg aagactgccc
    20161 gcgcagcgcc gggttttcca gcaccagcac gcggcgcacg gcctctttcg caccaatcag
    20221 gccgccgctc tccagtagca gcgggcggat ctcctgataa ttccagtacg ctggcgcaca
    20281 gttagcgttg ggcgttttcg gcaccagatg gtgcagtgac tcccacagcg gggtcaggtt
    20341 ttgcccggaa atatgctgat aaaattgctc gcggctattt tgattgttat cagtcattga
    20401 gatgctcctt attcgttcac aacgcctgcg cgaggcagtg agtcagggac agaggtcgac
    20461 tggcgaacca cggtaatcag cagggttaac atcaccgcac tgatcgccgc aggcacagcg
    20521 ataatgaaaa acagggtgtc gaaagagaaa ttcatcgcca tcatcacgcc gccagacagc
    20581 gaaccaacaa tcgcgccgca gcggccgata gcatttgacc agctcacgcc cgtggctcga
    20641 ctttgtgttg gataaagtgt tgcggtcagc gcattcagcc ccacctgcga tccgctaatg
    20701 ccaatgccag tgccgaaaat cgccagcgcc atcaaccata atccatcctg gcttaagcca
    20761 atcatcacga tgcatatagc gcctatggca tagctcagcg tcagcacgcg gaaaggattg
    20821 aacttatcca tcagcacacc cagcgccagc gcgcctaacg taccaccaat ctggaacgcg
    20881 gcggtgaccc aggacgcgtg ttgcaggtcg atgccgcgat ggttcagtag cgtcggcatc
    20941 cagcttgaga gcaggtaaat aatcagcagg ctcataaaga acactaccca cagcatcaac
    21001 gtgatgggta actgacgtcc cataaacagt tgacgaatgc tgccttttgt aacggaggct
    21061 gattcaatga gataaaagtg ggtatcgaca taacgctcgc gggtgatggc gctgacggtt
    21121 ttcgcaatca ccgcctgcgg tagctggcgg cgcacctgcc agcgtggcga ttccggcaga
    21181 actaccagca gtgcgacaaa cagcatcagc ggtaacacgc cccccaacac cagaatgccg
    21241 tgccagccaa tcaccggcac cagctgcgca ctgacaatcc cgccaaacgc cgaacccagc
    21301 gtaaagccgc aaaacatcag cgtcaccagc gcgccgcggc ggcgggcagg cagatactcc
    21361 gaggtcatgg tgatggtgtt cggcatcgcg ccgcccagac ccaggccggt gagaaagcgc
    21421 aggaagacca gcgtttgcag atccggtgag aaagccgagg cgaggcttaa cgcgccaaac
    21481 aaaaagacgc acagctcaat cacccgcttg cgaccaaagc gatcggacaa cgggccacaa
    21541 agcagtgcgc ctgcggttaa tcctagcaga cccgcgccaa aaagcggggc gagatcgcct
    21601 gcggtgagtt gccagtgagt gcgaatatca ggggcgataa atccgatagc cgcggtgtca
    21661 aaaccatcga gcattaccac cagaaaacag cagatgataa cgcgccactg catcttgccg
    21721 accggagcag catcgataag tgcttgtaaa tcacgtcgtt gagtcatagt aaatgcctca
    21781 gtgcaggtaa ggtagatgtt tcttttgttt tgtatatgtt gcgatattgt gattaaagag
    21841 atgagatgta caatggcatt tcgggcagag agataacctt gaggttatgg caaatggcga
    21901 actgggcgca aaaattaaaa ctacatcacc ttgaaatgct ggtggcgctg ggtgaacagg
    21961 gcaatctaac ccatgtcgca cgaatgatga atatcaccca acctgcgctt tcgaaatggt
    22021 tgtcacaact cgaagatgag attggaatca cactttttga gcggcacagc aaaggattac
    22081 gtccgactga aggcggcaaa ttgttattgc aacatgccca acggttaatc aatgacatgg
    22141 agcgatcgca acatgaactg gcccgtttta aacaaggtgg actggtcggc agtctgaaaa
    22201 ttggctgttc tcccgtcgcc actgactgcg tatcccaggc gattttgcat ttgcttacga
    22261 agatgccaac tctacacctg aatatcgaag agaaggtgat gaccccattg ctgcaagatc
    22321 tattggccgg ttctgtcgat gtggtcgttg ggcgaattgg cggtcgcgca ttgcagctgc
    22381 cactgaatta tcaggtgctg tacaccgagc cagtatgctt tgtcgcccgc cctgagcacc
    22441 cactggcgaa atacgccacc ctcagctgga atgatcttgc caactggcgc tggatcgtct
    22501 ggccgacggg tacccccatt cgcattagta ttgataatgc gctggtagac cacggcgtaa
    22561 tgctgccaga aaacacgatt gagtcggcgt cgatgaacgt cagtaccaac ctgttacaaa
    22621 gcagcgatat gatctctatt ttgtcattac gcctggcgca acgttatgcc agccagggac
    22681 aactggtgat actcaacctg ccaaagatag aacagaaagg tagcgtcggt gtgttctggc
    22741 gcaataacga aacgccttct tatgctttaa gtcagtttct gcacttttta cagcagaatt
    22801 agtgtgctga acggggcgct cgcccgaggt tacctcccta aaattcaact atgctgagaa
    22861 ttcttaatgt gatcggtagc acgtttcacg aattaattgt atgatgaatc catctcatct
    22921 ggggtgttga ttatgagtaa gacactgaac attatctggc aatatttacg cgctttcgtc
    22981 ctgatttatg cctgcctgta tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt
    23041 ccgggcagca tcatcgggat gctgatcctg tttgtcctgc tggccttgca aattcttccg
    23101 gcgaaatggg tcaatccggg gtgctacgta ctgattcgct atatggcgct attgtttgtg
    23161 ccgattggcg taggcgtcat gcaatatttt gatttgctcc gcgcacagtt tggcccggta
    23221 gtggtttcct gtgcaatcag tacgctggtg gtttttctgg tggtgagctg gagttcgcaa
    23281 ctggtacacg gtgaacgtaa agtcgtaggt cagaaaggat cagaagaatg atggcgaata
    23341 tctggtggtc attaccgctg acgttgattg tcttttttgc cgcccgcaaa ctggcggcac
    23401 ggtataagtt tcccctgctt aacccgttgc tggtagcaat ggtggtgatc attccttttt
    23461 taatgctgac cggcatctct tacgacagtt actttaaagg tagcgaagtg ctaaacgatc
    23521 tgctgcaacc ggcggtcgtc gcgctggcct atcctttata tgagcagcta caccagatcc
    23581 gcgcgcgctg gaaatcgatc atcaccatct gttttatcgg cagcgtggtt gcgatggtga
    23641 cggggacttc cgtggcgttg ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc
    23701 cgaaatcagt caccacgcct attgcaatgg cggttggcgg cagtattggc ggtattccgg
    23761 caatcagcgc agtttgcgtg attttcgtcg gcatcctcgg cgcggtattt ggccatacat
    23821 tgcttaatgc gatgcgtatt cgtaccaaag ctgcgcgcgg tctggcaatg gggactgcct
    23881 cgcacgccct cggtacggcg cgctgcgccg agctggatta tcaggaaggt gcatttagtt
    23941 cgctagcgct ggtgttatgc gggataatta cttcgctgat cgcaccgatc cttttcccga
    24001 ttattctggc agtaatgggc taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc
    24061 acgcgttgca taattaatga gattcagatc acatataaag ccataacggg ttcgtaaact
    24121 gttatcccat tacatgatta tgaggcaacg ccatgcatcc acgttttcaa accgcttttg
    24181 cccaacttgc ggataacttg caatctgcac tggaacctat tctggcagac aagtacttcc
    24241 ccgctttgtt gaccggggag caagtctcat cgctgaagag cgcaacgggg ctggacgaag
    24301 acgcgctggc attcgcacta cttccgctgg cggcggcctg tgcgcgtacg ccattgtcga
    24361 attttaatgt tggcgcaatt gcgcgcggtg tgagcggaac ctggtatttt ggtgccaata
    24421 tggaatttat tggtgcgaca atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc
    24481 acgcctggtt gagtggtgaa aaagcgcttg cagccatcac cgttaactac acgccttgtg
    24541 gtcactgccg tcagtttatg aatgaactga acagcggtct ggatctgcgt attcatctgc
    24601 cgggccgcga ggcacacgcg ctgcgtgact atctggcgga tgcctttggg ccgaaagatc
    24661 tggagattaa aacgctgctg atggacgagc aggatcacgg ctatgcgctg actggtgatg
    24721 cgctttctca ggcggcgatt gcggcggcaa accgttcgca catgccttac agtaagtcgc
    24781 caagcggtgt cgcgctggaa tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa
    24841 acgccgcatt caacccgact ctgccaccgt tgcagggagc gttaattcta ttgaatctca
    24901 agggttatga ttacccggat atccagcgcg cagttctggc agagaaagcc gatgcgccgt
    24961 tgattcagtg ggatgccacc tccgcaacac tgaaagctct cggctgtcac aatatcgacc
    25021 gcgtgcttct cgcttaagcc tggtgccgga tgcggcgtga acgccttgtc cggcttgcca
    25081 gccctctcct ggtgtcgaaa atcccggcaa acagtttgtc gtttcttgcg caaaaccagc
    25141 gggtaaagta gcctgatgga aattttcctt agatcgagtc tcctgcatgt taaagcgcgt
    25201 gttcctcagc ctgttagtcc tgatcggctt gctgctgttg actgtgctcg gcctcgatcg
    25261 ctggatgagc tggaaaaccg cgccttatat ctacgacgaa ttgcaggatc tcccctaccg
    25321 ccaggtcggt gtggtgctcg gaacagcaaa atattatcgt actggcgtaa ttaatcagta
    25381 ttatcgctac cgcattcaag gagcgattaa tgcctataac agcggtaagg taaattatct
    25441 attactgagc ggcgataacg cattgcaaag ttataatgag ccgatgacca tgcgcaaaga
    25501 tttaatcgct gctggtgtcg acccatcaga tattgttctc gattacgcag gctttcgtac
    25561 gctggattcc atcgtgcgta cacgcaaagt tttcgatact aatgatttca ttattatcac
    25621 ccaacgtttc cactgtgagc gggcattatt tattgcgctg catatgggga ttcaggctca
    25681 gtgttatgcc gtaccgtcac cgaaagatat gctgtcagta cgtattcgtg aatttgccgc
    25741 ccgtttcggt gcgctggctg acctttatat ttttaaacgt gaaccgcgtt ttttagggcc
    25801 gctggtccct attccggcta tgcaccaggt gccggaagat gcgcaggggt atcccgccgt
    25861 cacacccgaa cagttacttg aattacaaaa gaaacaagga aagtagttat ggatgtacag
    25921 cagttttttg tcgttgccgt ttttttcctt atcccgatat tttgtttccg cgaagcatgg
    25981 aaaggctggc gcgcaggcgc gattgataaa cgggttaaaa atgcaccgga accggtgtat
    26041 gtctggcgag caaaaaatcc cggactcttt ttcgcttata tggtggcata tatcggcttc
    26101 ggaattttat ctatcggcat gattgtttat cttattttct atcgttaata ttttctcttt
    26161 tctgttaatt attgagaata attattactt cactcaataa gctgcggata taacattctg
    26221 aaccgcagct atttgtgaat cctttcacag tttaaatttc cccacgcgca tagccttaat
    26281 atcagcacgc aatatttatt tagcgttcgc cttaattact catagcatta aggaagatca
    26341 catgccgcaa caaaattatc tggatgaact cactccggct tttacgccgt tactggcgat
    26401 taaagaagcc tctcgctgtt tattatgtca cgacgctccc tgtagtcagg cttgcccggc
    26461 acagaccgat ccggggaaat ttattcgctc aatctacttt cgtaatttta aaggcgctgc
    26521 cgagacaatt cgcgaaaata atgccctcgg tgccgtttgt gccagagtgt gcccgacgga
    26581 gaaattatgc caaagcggtt gtacccgtgc cggtgttgat gctcccattg atatcggccg
    26641 cttacagcgt tttgttactg attttgaaca acaaaccggg atggaaattt atcagcccgg
    26701 tactaaaacg ctcggcaaag tcgcgattat tggcgcaggt cctgccggat tgcaggccag
    26761 tgtgacgcta acaaaccagg gttatgacgt cacgatttat gagaaagaag cgcaccccgg
    26821 tggctggttg cgtaacggta ttccgcaatt tcggttaccg caatcagtgc tggatgcaga
    26881 aatcgcccgt attgaaaaaa tgggcgtgac cattaagtgc aataacgaaa tcggtaaaac
    26941 actcaccctg gagcaactga aagcggaaaa ccgcgcggta ctggtcaccg tggggttatc
    27001 aagcggttcc gggctaccgc tgtttgagca tagcgacgtt gagattgccg tcgacttcct
    27061 gctacgtgca cgacaggcgc aaggcgatat cagcattcca caaagcgcat taattatcgg
    27121 cggcggtgat gtcgcgatgg acgtagccag cacgctgaaa gttctcggct gtcaggcggt
    27181 aacttgcgta gcgcgtgaag agttagatga gtttccggca agcgaaaaag agtttgccag
    27241 cgcccgggag ctgggcgttt cgatcatcga tggattcacg ccagtagccg tggaaggcaa
    27301 taaagtcacg tttaagcatg tacggttatc gggcgaactg acgatggcgg cagataaaat
    27361 tattctcgcc gtcggtcagc atgccagact ggatgacttt gcgaagttag agccgcagcg
    27421 taacaccatc aaaacacaaa attatcagac ccgcgatccg caagtctttg ctgctggcga
    27481 tattgttgag ggtgacaaaa ccgtggtcta tgccgtgaaa accgggaaag aagccgccga
    27541 ggcgattcat cactatttag agggagcttg ctcatgttaa cgaaagatct ttcgattact
    27601 ttttgcggcg tgaagtttcc caacccgttc tgcctctctt cttcgccggt aggcaactgc
    27661 tatgagatgt gtgccaaagc ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc
    27721 ggctttttta tcgccaacga agtctcgccg cgttttgatc atctggtgaa agaagatacc
    27781 ggttttatcg gcttcaaaaa tatggaacag attgctgaac atccgttgga agagaatctg
    27841 gccgccctgc gtcggctgaa ggaagattac ccggacaaag tattgatcgc ttcgatcatg
    27901 ggggaaaatg agcagcaatg ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat
    27961 atgatcgagt gtaacttctc ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc
    28021 gggcaaagcc cggagctggt agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg
    28081 ccaatgctgg cgaagatgac gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg
    28141 aagcgcggcg gcgcagatgg cattgcggca attaacaccg ttaaatccat caccaatatc
    28201 gatcttaatc agaaaatcgg tatgccgatc gttaacggaa aatcgagtat ttccggatat
    28261 tccggtaaag cggtaaaacc gatcgccctg cgcttcattc agcaaatgcg cacccatcca
    28321 gaactgcgcg atttcccaat cagcggtatc ggcggcattg aaacctggga ggatgcggcg
    28381 gagtttttat tgctcggcgc agcaacgtta caggtcacca ccggcattat gcagtacggc
    28441 tatcgcatcg tagaagatat ggcgagcggg ttatcgcatt atctcgccga tcagggattt
    28501 gattcgttgc aggagatggt aggtctggcg aataacaata ttgtcccggc ggaagattta
    28561 gaccgcagtt atattgtcta tccccgtatc aatcttgata aatgtgttgg ctgtggacgc
    28621 tgttatattt cctgttacga cggcggtcac caggcgatgg aatggagcga gaaaacccgc
    28681 acaccgcatt gtaataccga gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg
    28741 gtgggttgta ttgagctcgg ggaagtgaag tttaagaaag gcgagaaaga acacccggta
    28801 acgttgtaaa accagagacg catccggcat ttggttcctg tcgccggatg cggcttgaac
    28861 gccttatccg gcctacaaaa ccatacaaat cagtatgttg agctcattgc aggccggata
    28921 aaacgcgcca gcgttgcatc cggcatttgg ctcctgtcgc cggatgcggc gtgaacgcct
    28981 tatccggcct acaaaaccac acagatcatg atgttatgtg cgtttatgac cgaatgcgga
    29041 ccacaatcat ttcacttctt acgcgcgtac ttcagtgaat ccagcgctac ggcgaagata
    29101 ataatcgccc ctttgatgat gtactgccag tatgggttta cgccgatgta cgtcaggcca
    29161 tagttgatga cggtaaaaat aattaccccg gtcaccacgc caatcaccgt ccccacaccg
    29221 ccgctgaacg atacaccgcc taccacgcac gccgcgatag catccagttc atacataaag
    29281 ccgaggttgt tggtggcaga gccgatacgt ccggcttcta acatcccgcc aaaggcatag
    29341 aacacgccag acaacgcgta gatcatcagc aggttcaggc cgacgttgac accagatact
    29401 tttgccgctt ccgggttacc gccaatggca aaaatgttct taccgaagcg ggttttgttc
    29461 cacaacaccc agacgaacgc caccgcaatc aacgcgtaga aggtgatgta agagagacgg
    29521 aaactcccca gcgcgacaaa gccctgagca aaggtagaga agccactgtc aaaaccagaa
    29581 attggcgacg ccccgacaaa gtcgtaataa agcgagttga tgccatagac gatgatcatc
    29641 gtgccgagcg tggtaatgaa cggcgtcacg ttgagataag cgataatcag accgttgatc
    29701 aaaccgatca ccgcaccaat ggcacagaca atcagaataa ccagcgcaat cggcatcgtc
    29761 gccatttccg ggaacacttt gttggcgtta tccatggact gcaataatgt cgctgccacc
    29821 actgccgcca gccctacttg acgccccgca gaaaggtcgg tcccctgggt gacaattaac
    29881 cctgccacac cgagcgcgat aataatacgc accgatgact gggtgagaat attacttaag
    29941 ttcaacagac ttaaaaatgt tggggcctgg aaaataataa tcgcaagcaa aactaaaaga
    30001 acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac ttttcttatt taacgcactc
    30061 atgggaagcc cctaatctta aaggtgcaaa gacgcaagac gtagaatttc gttttgcgtt
    30121 gttgtttttg tatcgacaat tccggaaacg aggccattgc tcataaccag aatacggtct
    30181 gttatcccta acaactctgg catttcagag gagataataa taatcccctt gcctttcttc
    30241 gccagttcgg caattaactg ataaatctca aacttcgccc caacatcaat accgcgcgtc
    30301 ggttcatcga gcattaatat ttctggttgc gttaacagcc agcgcccaat aatcaccttt
    30361 tgctgattac cacctgagag cgaaccaatt tgcgtccgat gccccggtgt ttttacccgc
    30421 atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg agttatccag taaaccaact
    30481 ttatttttgt agttgcgaat attggaaatt aacgagttaa aaccaatatc cagataggca
    30541 taaattcccg ttgagcggcg ctcctcagtt accagtgcaa atccgtggtt tatggcttcg
    30601 ttggcattat ggttattgat ctttttgccg tgcaacgtaa tggtgccagc cgatttctcg
    30661 cgaataccaa ataacgtctc aacaatatcg gtacgtttcg cccccaccag accggcaata
    30721 ccgaggatct cccctttatg cagatcaaac gagacatcgc gaatcgacgg ctggcgcagt
    30781 gacgtcaggt tacgtacctc gaggatgact tcgcccggct tgttttcttt gtcaggaaaa
    30841 cgctggttaa gagaacgccc aaccatcatg gcgataatct tgtccatcgt cagccctgcc
    30901 agcggttcgg tggcgatcca ctgaccgtcg cgcaatacgg taacttcatc acataactgg
    30961 aagatttcct ccattttatg cgagatataa acaataccgc agccgcgctc ttttaattta
    31021 cgaataatag tgaacagatg attgacctct ttttcggtta acgaagaagt cggttcatcc
    31081 ataatcacaa ttttcgcgtt ataggaaaac gctttggcga tttcgatcat ctgcatttgc
    31141 gaaacagata atgtgccgac gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa
    31201 atcgctttgg tttcgcggta cattttgtcc tgatcgacaa acatgccttt ggtggggtat
    31261 cgcccaagcc acatgttgtc catcaccgaa cgttgtaata ccaggtttaa ctcctggtgt
    31321 accatcgaaa taccattttc cagtgcttct ttggccgaat ggaaatcgat ctctttaccc
    31381 tggaataaaa tggtgccgga gtctttttta taaataccaa acaggcattt taataatgtt
    31441 gattttcctg cgccgttttc ccccattaat gcatggatag aatgtggccg gacttttaaa
    31501 ttaacgttat caagtgcctt aacaccagga aaagacttgt tgataccgct catttccaac
    31561 aagtattccc cggaagacgg agtcgttgag ctgaccatat aattttacct tgttggccat
    31621 acaataaggg cgcagtaata gactgcgccc aatcagtctt atttcttgct gaattcagcc
    31681 aggttgtctt tatctacgcc aacataaggt acgcggacca ctttgttgtc gattttccag
    31741 ttggtgccat cagccgcacc tttaccatcg gccaggtttt tcgccagatc aaaggtcgct
    31801 ttcgcctggt tgttagcatc gttcagtacg gtgcccgcca gtgcaccgga tttcaccagc
    31861 gccagcgctt ctggcagagc atcgacgcca aacaccggaa tgctggactt gttgtgtgct
    31921 ttcagtgctt ctaccgcgcc cattgccatc gcatcgttgt tggcgataac cacttcgatt
    31981 ttgttggcgt tcgggccaga cagccaggcg tccatcttat ctttcgcctg agcggtatcc
    32041 cacatagcgg tatctaactg taactgttca gttttaatgc ctttgtcgtt cagctcttta
    32101 atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg gttcaccttt cagcagtacg
    32161 aattgaatct gaccgtcttt attcagatcc caaccctgat tcgccgccca gtgtttagca
    32221 atcaaatcgc cttgaataat gccggactct ttggagtcag tgccaacgta gtaggctttg
    32281 tcgtagctat ccagcgcctt acgagacggt tctttgttaa agaataccac cggcacattt
    32341 tgcccacgcg ctttctcaat caccgtaccc gcagctgccg ggtcaaccag gttgattgcc
    32401 agtgccttca cccctttcgc cagcaatacg tcgatctgat cgttctgctt ggactggtca
    32461 ttctgtgaat cattcatcag cagctgaaca tctggcgcgg ctttcgcatc ttgctcaata
    32521 gccttgcgca ctacagacat aaagttatcg tcgtacttat agattgttac accaatgcga
    32581 gtatcagcag cgtgtgcagt ggcaccgaat aacatgctgg ccatcacagc agacagggtt
    32641 aacaccttct tattcatggt atctccggtt tttcttatgc agggtagtgc ttgagataaa
    32701 tgctcggcgg ggcagtagag ttaatgaagt gttactgaac gccgaagctc actttttaaa
    32761 attcgttctt ccatgcccgg taacgctcca gaaaacggct ttaattgttg ttatgacgct
    32821 attacctcgg tgaaagtgat tattcactgt tacatacggg ttacaacgtt aaaacggtgc
    32881 aatcatagct atcacattgt taagatactg tgaaatcact cacagattga aagcggttac
    32941 atcgcctgat ttgttgagtt agtgatcgcc gccgcattct ggcgtgttgc gacagaatgg
    33001 cgacgcacta acgtcggcat aaaacagtgg ctggcacgag gatcaatatt gcctgctgcc
    33061 ccctgcaagg ccagttcggt ggctaatttc gccattgaag caatgggata acgcacggtc
    33121 gttaattgcg ggtcggtgta acgggcgatg ggaatatcat cgaaaccgat gattgagaga
    33181 tgtaacggaa tcgcaatgcc attatctttt aatgctgtca gtgcgccagc agccatattg
    33241 tcgttataag caaatacagc ggtaagttgt agattgcgcc ccagcagttc aaccatcgcc
    33301 gcctcaccgc ccggcatgtc cggcgtacca gtgccaatcc agctttccgg cggaataata
    33361 tcctgctctt tcaacgcact catccagcct gctttacgca tggcgtcatc ttcaatgccg
    33421 tggctggaag aaagataacc aatacgttga tgaccgttat tcagcaacat gcgcgtcgcc
    33481 attcgggcac cgctgagatt atccaggcaa acgcaacgat gggtgtaccc cggcacaacg
    33541 cggttgatta acaccatacc gggaatgtta tccataaatt gcgccagttc atcgtcactc
    33601 aatgcttttg agtgaacaat caacgcatta caacgctggc gaattaacac ctcaatggcg
    33661 tgacgctctt tttccgcttc atgatagcta ttgccgatta gcacgtattt ctgatgctgc
    33721 tgagcgacca gatccaccgc ttttaccagc gcgccgaaaa acgcatcaga aacgtccatc
    33781 accaccacgc caatggtgtc gctaacctga gttgccagcg cctgggcatt ggcgtttggc
    33841 cgataatcca gctcactcac ggctttcatt actgcttcac gcgtgtcggc actgaccagc
    33901 gtgctgttat tgagcacccg ggaaaccgtt gccacagaga cgccagcctg acgcgctaca
    33961 tcacgaatgg tgatcatatt cactacctgt tatgagattg cagtaaatga ctgcttgctg
    34021 ccggctattt tgtcagcact gaatacagga cttcgtgaat cgagtcacag caatggaaac
    34081 ggttacagcc gttttgttaa cgaatgtgat ccagatcgtt atcttgatgt tttagatatt
    34141 gccggtccgg cagcacgcaa agttaactga cgccagagcc attccaccgg cccctggcgg
    34201 aagtaacgca gccagataac agagaagaga atattcgcca gccataccgg aataacaaac
    34261 gccagcaatt ccagacggtc aaactgcata aacagaccga ggtggtaaaa aagcgtggta
    34321 caaatcagcg tttgcaatag atagttggtc agcgccatcc gtccgacgca ggcgatcgcc
    34381 agcaccagct taaagcggct caattgcggc cagaagccat aaaacagcga cgcatagcca
    34441 atcgcctgaa acggcgcact cagttcccgc ggcatttgaa gtaagaaggc acaccagcga
    34501 tacgcccagt ccagctgcca ttgcagggcg atggcaggaa ggttaatgat caccccaatc
    34561 gccactagca caaaaccagt acgacgatag tgacgtaagc tgaactgccc tttcagccag
    34621 ccactgcgca tcaatgcggc accaatgagc atcatcccag ccagttgcca gccatactgt
    34681 gcgcccagtg ccaataaact gttcccaaca ccatcggcac gattactgat cgcttcaacg
    34741 ccgccgtgaa gcttccagta tttttcatat aaaatagccg atgcatccgg cgtccaggcg
    34801 cggctagtct ggctatcgga aatcaacccc aataacagca aaacgccaag ccccaccaga
    34861 taaagcatga cgccggtatt aaaaaggctt tttaccgatg gcgcatcgcg caccagccgc
    34921 cagcagatta agcccaccag cccgtaagcc agcagaatat cgccgtccca gaacaataaa
    34981 ccgtgaataa agcccagcaa gactaacagc gttaaccgcg actggatcca gcgtctgcca
    35041 cggggcagca acatttgcag gccagcacca aacagcagcg caaaaagcgt gaggaatttc
    35101 acctggccga tgagatcgag aaatgcccag gtccatgcat cctgcggcgt aatagcgccg
    35161 taccaggcgg gattaagata agccgccttt ggtagcccaa aggcgctgat gtttaatagc
    35221 aggatcccca gaatggcgac gccgcgaaca aagtcgagcg tgacgttgcg ctccatggtt
    35281 cctgccttta aatcagttgt gatgacgcac agcgcgcaga aactcgtggc gcgtattctg
    35341 actggatttg aacaatccac caagagaggt cgttgtcgtg gcactggttg catcgcggat
    35401 gccacgcgcc ttcacgcaat aatgcaccgc gtcgatagag acagccacgt tattggtgcc
    35461 cagcagcgtt tgtagcgcaa tgagaatttg ctgcgtcaga cgttcctgca cctgcggacg
    35521 ctgggcaaag aactgcacaa tacggttaat ttttgacaga ccgatcaccg aatctttcgg
    35581 gatataagcc accgtcgctt tgccatcgat ggtaacaaaa tggtgttcac aggtactggt
    35641 cagagtgata tcgcgcacgg tgaccatttc atcgaccttc attttgtttt caatgagggt
    35701 gattttcggg aagttggcgt aatccagacc ggagaaaatt tcatcgacat acattttagc
    35761 gatgcgatgc ggcgtttcca tcaaactgtc atcagccagg tcgagattca gcagctgcat
    35821 gatttcggtc atatgaccag caataaggct tttgcgcgtt tcgttatcca tttcatgcac
    35881 gggcgggcgc agcggtgttt ccagtcctcg cgcaactaac gcttcatgaa ccagggccgc
    35941 ttctttactg agtgatggca tttatgattt ctcctgcagg tgtgacgcct ccgccctgcg
    36001 tgggggcaaa gttattaagc tgatttacag cctgattatt gtgcgtgagg cggcgcacat
    36061 aatccagtat tcacagcgat aattattgta attgccgctg cctttcatca gcagatgtta
    36121 aaacatcgtt atgcaaatac ggaagtgaaa gttactcaca gcacattgaa taaacggtat
    36181 gatgaagaaa ttgcaaacaa cacaacaagg agccacgcat ggaaatgctc gaagagcacc
    36241 gctgttttga aggctggcag caacgctggc gacacgactc cagtacctta aactgcccga
    36301 tgacgttcag tatctttctc ccaccccctc gtgatcacac cccgccacca gtgctgtact
    36361 ggctttccgg attaacctgc aatgacgaga acttcaccac caaggcgggt gcccagcgcg
    36421 tggcggcgga actggggatt gtactggtta tgccagacac cagcccgcgc ggcgaaaagg
    36481 ttgccaacga cgatggctac gatttaggcc agggcgcagg cttttatctt aatgccacgc
    36541 aaccgccgtg ggcgacgcat taccggatgt atgattatct gcgtgatgaa ttaccggcgc
    36601 tggttcagtc gcaatttaat gtcagcgacc gctgcgctat tagcggtcat tcaatgggtg
    36661 gtcacggtgc gctgattatg gcgctgaaaa acccgggcaa atacaccagc gtttcggcct
    36721 ttgcgccaat tgtgaatccg tgcagcgtcc cgtggggaat caaagcgttt agccgctatt
    36781 taggtgagga caaaaatgca tggctggaat gggacagttg cgcgctgatg tatgccagta
    36841 acgcgcagga tgcgatcccg acgcttatcg atcagggtga taacgatcag tttcttgctg
    36901 accaactgca acctgcagta cttgcagaag ccgcgcgcca gaaagcatgg ccgatgacgc
    36961 tgcgtattca gccgggatat gatcacagtt actacttcat cgcctctttt atagaggatc
    37021 acctgcgctt ccatgcgcag tatttactga agtgaaagtc cgcccggttc gccgggcatc
    37081 ttctcatcag aagcgataat ccactgccat aaagtaacga cgtccgtctt cgttatagct
    37141 gtagtcgtca cgactgagat ccttgtcgcc aaggttcagc acgcctgcgc gcagtttgac
    37201 gtctttagtc acctgccagg ccgcgccggt attccagatg gtataaccgc ccggtgtttt
    37261 agccgtcgcg ctgtcggctc gtttctgccc ggtatagtga ccagaaacat agaatgacca
    37321 gtcttccagc gccagcggtt tccagtccag cgtaccgtta gcggtatgga acggcagatc
    37381 ggatagcggt ttgttttcgc cgttgctgac atcacgacca tcgttgtagg tgtagttgag
    37441 tgacagtttc cattcatcgt tgaacggaat tttcagctcg gtttccacgc cctgaatacg
    37501 tgctttgtta acgttgtagt agctaaatac cggcacaccg ttcgctgtaa aaccaacaaa
    37561 gttttggtag cccggtgcag cattgacgtc agacgtacgg ctgatgctga tacgatcttt
    37621 cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc cttcttcacc
    37681 catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat ccgggctacc
    37741 cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa gttgcaacag
    37801 agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt cggtggcgtt
    37861 ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat cgtccatacg
    37921 cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca caaacagcgc
    37981 gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg catcgctaag
    38041 tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg tcagcggcaa
    38101 cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt tgccagggtt
    38161 tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat cccaacgccc
    38221 attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg agtcggaatc
    38281 acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat tcggtgtcca
    38341 ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg tttctccggt
    38401 atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc tgccgtaagc
    38461 tttcattccc agcacaccat caattaatgg tccactggta aagaactgac cgttataggt
    38521 atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac ccgaccattt
    38581 ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg aaccgtacag
    38641 cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg ggatccagtt
    38701 cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttaacgcgtt taccgtcgac
    38761 aagaatcagg gtgtagctgc tgtccagacc acgaatacta acgcccttac ggttatcccc
    38821 ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat tctgtaccgg
    38881 ttttcgctgc aggtcttcct gggtaatgac gctgatactg gcgggtgcat ctttaaggtt
    38941 ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga ccgctaacac
    39001 aggccatgca caagaaatag cggacaaaca cagcccgacc cgtacgaaag ggttcaacct
    39061 aaacattcca tatctccatg aggtaactac gaaaataaaa tgggttatcg ctcacatctt
    39121 cttcacgtcc ccttgcgtac ggcagcatcg cggtggactt atttgtatgc aggtgatcat
    39181 cccagaaagc cggaccagcc tctgatttgt cagccttttt tgattgcggc gtaacgataa
    39241 tgcaaacgat aacaattatc aatccaaatg ttaaatttta tatccgcatg atttgtcagg
    39301 gaaatggaat tgaaaaagcc ctctcggttg agagggctta gcaaggaagg gagaaactta
    39361 tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtggg ttcctttaat
    39421 cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg ttgccgctac
    39481 gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca aagtgataat
    39541 cagacacaga atgaatgcga agatcggccc caatgggaag aaacctgaac ggtacggcag
    39601 atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt ggctaatggc
    39661 aatccccagc caggcgataa aaccggtcat cccggaggta ttcagcagcc acaggtatac
    39721 cgtctggttg ccaaacatgg aggtcaggaa gcacagaccg gcaatcaccg tcgtcgcata
    39781 cagcgcatta cgcggcacgc caccacgcga cagtttagcg aaaatgcgcg gcgctttacc
    39841 gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt tacccgctga
    39901 cagcaccgcc gtcagaataa ctgcgttcat caccgccgca gcagagagca gacccgcgtg
    39961 ctggaacacc agggtgaacg gactaacgct gatgtcttta acatcgttac gcagcaggct
    40021 cggatcggtg tacggaataa tcaggctgat aatcaggatc gcgaacacat agaacaacag
    40081 gattcgccag aacacctgac gtaccgcgcg tggaatgttt ttccccggat cttcggactc
    40141 gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa tcatagctac
    40201 gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc agttgctcca
    40261 gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa cgatgataaa
    40321 gacaataact gtcgtgactt tgatcagtga gaaccagtat tccgcttcac caaagccgcg
    40381 aacagagatg tagttcagca ggaagataac gccgaggaac aacgcactcc agatccagcc
    40441 cggtgtatcc gggaaccacc agctcatgac cagctgagct gcaaccaggt caacggcaat
    40501 agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc cctcttcaac
    40561 atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag ccagttcacc
    40621 gagactggtc atcaggaagt aaaccatcag gccaatcaac atatacgaga gcaatgcccc
    40681 gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg taccgatgga
    40741 accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta agcccggcgc
    40801 ttctgtggtt ttagtttcgg aaaccataca aaaatgctat ccatctaaaa aatgaggcgc
    40861 gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa gacaccttca
    40921 tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat gcatgctgaa
    40981 ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcaa cttaacgcgg
    41041 cacatttgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa ggtgtttttg
    41101 ccgatgatgt atacgccaca acgtacgcat caggcgcggc agagggaccg caacttcact
    41161 taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac taattcccaa
    41221 cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg ccatctcaaa
    41281 cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg aaccgcgttc
    41341 acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac gggccaacgg
    41401 cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag aaatgatttc
    41461 agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca gcaccgcttg
    41521 catcacgtcc tggctattcc caacgctaag ttcaatcggc aactgcggat aatcatggcg
    41581 ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg catagatacg
    41641 aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt caagcaatgc
    41701 caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt tccccacgcg
    41761 atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc tcactgctga
    41821 ttgcgacaac gccagcatca ccgacgcttg ggtggttgat ccacttttca atacttctgc
    41881 aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat aaagattaat
    41941 tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat gcccggttaa
    42001 aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg tggcatttta
    42061 ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt ggttccattc
    42121 ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg gggatggttt
    42181 taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg ctgtttgcca
    42241 agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg ttctcgcaaa
    42301 ttgccgatgt cggtatcagt gggatcatca ttgacgtgct gacgctgtcc agtaccttcc
    42361 tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc agctggttga
    42421 tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag ccggtagtga
    42481 aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc gggaccgtcg
    42541 cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt ccggaaactt
    42601 tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg gcaggtcatg
    42661 ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc gtgatgatgc
    42721 tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg gcgaacagcg
    42781 gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgta gttgccatct
    42841 ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg ctggatacct
    42901 tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc gcgctgaaaa
    42961 aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg attgttggtg
    43021 gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca tcttgctcct
    43081 gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc tcgcatgaaa
    43141 tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat tcgcgccgcc
    43201 gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg gcgtgccgca
    43261 ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata tcactacaca
    43321 tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc ggtcactgaa
    43381 gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga acagctgggg
    43441 ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga agaggattgc
    43501 cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg tgtgacagcg
    43561 gtgatagaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga acatctcgcg
    43621 gcgattatcg acggcgtgga agataaatcc cgcgtcggcg tctgcattga tacctgccat
    43681 gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgagaaaac attcgcggat
    43741 tttgcccgta ctgtcggctt taagtatctg cgcgggatgc accttaacga tgcgaaaagc
    43801 acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat cggtcatgat
    43861 gcgttccgct ggatcatgca ggacgaccgt ttcgacggca ttccgctgat cctcgaaacc
    43921 atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca aactgaaaaa
    43981 gcggtagcct gaagatgaat aaccgggaaa aggagatcct tgcaatttta cggcgtaacc
    44041 cgctgattca gcagaacgaa attgcggaca tgctgcaaat cagccgttcg cgcgttgcgg
    44101 cgcatattat ggatttaatg cgcaaaggcc ggattaaagg caaaggttac attctcaccg
    44161 agcaggaata ctgcgtagtg gtggggacaa tcaatatgga tattcgcggg atggcggata
    44221 tccgttaccc gcaatcggct tctcatcccg gtacaattca ttgctcagcg ggcggcgtgg
    44281 gacgcaacat cgcccacaat ctggcgctgt taggccgtga cgtccatttg ctttcagtga
    44341 ttggcgatga cttttatggc gaaatgctcc tggaagaaac gcgccgcgcc ggcgtgaatg
    44401 tctccggctg cgttcgttta catggtcaaa gcacatcgac gtatctggca attgccaatc
    44461 gagacgatga aaccgtgctg gcgattaacg atacccatct gctggaacag ttatcgccgc
    44521 aattattgaa cgggtcgcgc gatttacttc gtcatgcggg cgtggtactg gctgattgca
    44581 acctgacagc cgaggcgctg gaatgggtct ttaccctcgc tggtgagatc ccggtgtttg
    44641 tcgataccgt ttcagaattc aaagcgggca aaatcaaaca ctggctggcg catattcaca
    44701 ccctgaaacc cactttaccg gagctggaaa ttttatgggg acaggcgatc accagcgatg
    44761 ctgaccgtaa tgctgcagtg aatgcgttgc atcagcaagg tgttcagcaa ctgtttgttt
    44821 atttgcccga tgagtcagtt tattgcagcg aaaaggatgg agaacaattt ttgctgaccg
    44881 caccagcgca tacgacagta gacagttttg gtgctgacga tggttttatg gcgggcctgg
    44941 tatatagctt tctggaagga aacaatttcc gtgacagcgc ccgttttgcg atggcctgcg
    45001 cggcaatttc acgcgccagc ggcagcttaa acaaccctcc cctgtctgcc gataacgcgc
    45061 tttcattagt gccaatggtg taacaatgtt gccggattcg acgcttgacc gcatcttatc
    45121 cggctcataa atcgcacact acgctaaacc aataaagaac ccggcaatgg tggcactcat
    45181 caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca agctgggcga tttccggcgc
    45241 acggtgtggc gcaaccgcag aaaacgcccc caccaccacc ccgatagaac caaagttagc
    45301 gaaaccgcac aacgcgaagg aaataatcgc cacggtttta gcatccagag tgccagccgt
    45361 ttgcagatag ggtgagaaat tgagataagc gacaaattca tttattgcca gtttctgtcc
    45421 aatcaaactc ccggcaagat tcgcatcact ccagtcaaca cccattaccc acgccagtgg
    45481 ggccagcaga taacctacaa tggactccag cgaggcatgt tcaaaaccaa accagccgcc
    45541 aacgccgccg ataataccgt taattaacgc aatgatggcg acgaatgcca tcactactgt
    45601 cgccacacct gcggcgattt tcagcccggt cattgcccct gtcgcggcgg cttcaataat
    45661 gctttttggc ggtgtttcgg tgaaagagag gttattaaaa gaaacctgcg aagattccgt
    45721 agccgggctt agcaggcggg caaacaagat ccccccgggg atcgccatta acgatgccgc
    45781 cagcaaatat tccacaggca cgcccagtgc ggcataacca atcattgtcg aaccagcaat
    45841 cgaggccatg ccgctacaaa tcgctgtaaa taattcattg cgattcagcc gatcgataaa
    45901 gggcttcaca atcgccggaa tttcgttttg cccgaggaaa atggtagtga ccgcgacgaa
    45961 tgactcaatc ttgctgatat ttaatgcttt ctggaatata ccgccgagaa tgcgaattaa
    46021 aatccccatc acaccgatgt agtagagaat actcaccagt gcagtgacga agataattgc
    46081 cggtaatacc ctgaaaccaa agataaatcc tgcgccatca aataaggtgt ccattttcgg
    46141 tccgaccaga gaaccgaaga taaatgcgct acccgcgtcg ctgtacgcca tcactttatg
    46201 cacaccaaaa gcgacttttt cagcgaccca acgccctggc ggtaaccaaa gcataatgcc
    46261 gccaatcacg acctgtaaca ctaacgccgc gccaacggta cgcaggctga tcttcttctt
    46321 gtttactgac agtaaaaacg caatcgtcag caataccacc attcccagaa cacttctcat
    46381 gacatccata gtgattatct cttcatgtca tattgggcga cggctttctg cccatcagaa
    46441 atagccaggc ctgcatcttg cgataccggc ccggcttgtt atcaacactt aatgggtttt
    46501 gatgtagccg cgcacgcact cttcgactaa gccccagaac cagtcgctat caatagtgat
    46561 gccgactttg gtattagcgg gcttgccaag aacgcccagc tcgtcgcaga cggtacgccc
    46621 ataacaaggg ccactgttga cgtccacttc gacgtacatc tcctgggttt taatgccatc
    46681 agggttaatc agataaccga tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta
    46741 gttttcgaac tgggttttga gagtgaagtt catgatgtcg ctgaacagct ctccggcagg
    46801 gccgcctgcc ctttccattc gtgcaatcac gtccggggtg caaacggtct ggttggtgag
    46861 atcgaggccc atcatcacta atggaacgcc agaggtgaac accacgcgtg cggcttccgg
    46921 gtcagcaaag atgttgaact cggcagatgg cgtgaagttg cctgtaccgt aagcgccgcc
    46981 cattagcaca atttcacgga ttttgggcag gatcgcgggt tgcatacgca ttgccaccgc
    47041 gatatttgaa agcggaccaa ccggcaccag agtgatatcg ccatcgcttg ccatcagggt
    47101 atcaatgatg tatttcaccg catgagtgct ttctgcctgg cgggtcagcg gctcgaatac
    47161 cgggccatcc agtccggtat caccgtgaat attatcggca acgatttgtt gccgcataat
    47221 gggttgtggc atccccgcat aaaccggaac attaatctcc agtttctggc aaacattcag
    47281 gccattaatt aatgttttat caagcgtctg attacccgct acaatagtga tgcctaataa
    47341 atctattgcc ggatgtttcg ccgccatcat tatagcaata gcatcatcat gacccggatc
    47401 acaatccaga ataatttttc tcttttccat tgtttatttc ctctgtttcc agttgcgtta
    47461 ttttttctac agcaaagaaa tttcgatcgg gccgatgatt gaatcttaac aacagcgtac
    47521 gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat
    47581 gatcttaagc agcaattgat gagtgaatcc gcgtttaagg attgcttttt aacggatgtt
    47641 tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa
    47701 cagccgtcct ggctgtttta cctgacgcga ggccgcgcca ggctttacgc cacgctagct
    47761 aatggtcgcg tgtcgctgat cgatttcttt gccgccccct gttttattgg tgagattgag
    47821 ttaatcgata aagaccatga accgcgtgcg gtacaggcta ttgaagagtg ttggtgcctt
    47881 gcgctcccta tgaaacatta ccgtccgctg ttattaaacg acaccctatt tttacgaaaa
    47941 ctttgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa
    48001 tcatttccgt tagtgaatcg cctggcagca tttatattac tctcacaaga aggtgatctt
    48061 tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta
    48121 tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa aggatatcta
    48181 attaaaaaca gaaagcagtt gtcagggctg gcactggaga tggacccgga gaataaattc
    48241 tccgggatga tgcagtaaaa attatttgca atagcgcgat tgccggatgc aacgcttgat
    48301 gcgttttatc cggtctacaa attgagcatt acgccagacc aataaagaac ccggcaatgg
    48361 tcgcgctcat caggttggaa agcgttgctg ctgccagcgc ccgtaaacca agctgagcga
    48421 tttccggtgc gcgttttggc gaaatagccg aaaatgcccc aacgacaaca ccgatagaac
    48481 caaagttagc aaaaccacaa agcgcaaagg agataatcgc aatggttttc acttccagcg
    48541 tgccgcccgt ttgcaggtat ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca
    48601 gtttctgccc aatcaggcta cccgcaaggt tggcatcact ccagtccaca cccatgatcc
    48661 acgccagcgg tgccagcaca tagccaaaaa tactttccag agaagcattg gcgaagccaa
    48721 accagccgcc aattccgccg ataatgccgt tgatcagcgc aataattgcc acaaatgcca
    48781 ttaccaccgt cgccacacca gcggcgattt tcagcccggt catcgcaccg ctcgccgctg
    48841 cttcgataaa gctttttggc ggcgtttcgc tgaacgacag attttcaaac gtgacctgcg
    48901 aaagctcggt tgctggacta agaatacgtg caaacaaaat accgccgggg atcgccatca
    48961 gcgatgccgc caacaggtag tcaattggta cgcccattcc ggcataacca atcatcatcg
    49021 aaccagcaat ggacgccatc ccgctacaaa tagccgtaaa caactcgttt cgattcatgc
    49081 gatcgataaa cggtttaacg atcgccggga tctcattttg cccgaggaaa atagtggtga
    49141 ctgcgacaaa agattcgatt ttgctgatgt tgagggcttt ctggaaaatg ctgccaagga
    49201 tgcgaatcag cagccccatc acgccaatgt agtacagcag actaatgagc gcagtgacga
    49261 agataatcgc cggaagtacg cgaaaggcga agataaaacc cgcaccgtca aagaggacat
    49321 ccattttcgg cccgaccagc gaaccaaaaa tgaaggcgct accagcatca ctgtaagaca
    49381 tcactttatg aacgcctaat gccgcctgtt ctacagccca ctttcccggc ggaaaataga
    49441 gcatgatgcc accaagagca atttgcagca gtagtgcggc tgcaaccgta cgcaaactga
    49501 tgctcttttt attcactgac aacagaaatg ctattgccag taacaccacc atccccacaa
    49561 cacttctcat tatgtccata atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc
    49621 aggtttcggt atgtttgatg agtacgggcg acggctttct gcccgtcaga aattaacccg
    49681 cgagacgctg atattctttg gcaatttcgc tcgccagaat ggcgttgttg aacacaagct
    49741 ggatgttgga tttcaggctg tcaccgccgg tcagctcagc aacgcgcgcc agcaggaatg
    49801 gcgtactttc tttaccaata acaccctgcg cttcagcttc agctaccgcc tgatcgatca
    49861 ccgcattgat agtgtgttct ggcatcgcaa actgttccgg gatcgggttc gctaccacga
    49921 ggccaccgtt cagcccgctt tgccatttca ccgccattgc acgggcaatt tcactggcgc
    49981 tgtcgagacg aatgctgacg tcaaacgggc tggtacggca gaaaaacgca ggcagcgctt
    50041 tagtctgata gccaattaac ggcacaccga aggtttctaa atactcagtg gttaatccta
    50101 aatcgagaat agatttcgcc ccggcacaaa caacggtgac attagtattt gccagttctt
    50161 gcaaatcggc agaaatatcg aaggtatgtt ccgcccctcg atgaacacca ccaattccgc
    50221 cggtggcaaa tactttaatt ccggcaagcg ccgcaataat catcgttgaa gccacggtgg
    50281 ttgcaccatt ttttcctgcg gcaacgacaa aaggtaaatc gcgacgacta actttagtca
    50341 cgttatgccc ttcacgaccc agtaattcaa tttcttcttt gcttaaaccc actttcatca
    50401 cgccgccaat aatggcgata gtggcaggta ctgcgccctg tttacgaata gtttcttcta
    50461 cttcaattgc ggtctgggca ttttgtggga acggcatccc gtgagaaata atggtcgatt
    50521 ccagcgccac aaccggtttt ttgtttttta aagcgtcctg cacttccggg gaaatttgta
    50581 ataattcagg ggaaattttt aattcagaca ttctgcgttc tccactaacg atataacgtt
    50641 ggcaatcgat aaatcggggt tattggtgta ttcacaggag agcgccattg acgaacatcc
    50701 ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct acccaacacg aagcaagtcc
    50761 cgccatcatg gcatcgcccg ctccggtaac attaataaca ttggttttga tcggcgcaga
    50821 ccagccactt tcaccgctga tatcgctgta ataaacgccg tcgccgccca tgctcaatac
    50881 cagtcggttc aggccatgtt gatggaacca ggcagcaact tttgccacat cttcacgccc
    50941 tgacagcgca atcccactca gggtttccgc ttcaaggcgg tttggcttga gagtatggat
    51001 ttgacttaat cgctcgcgta ctttgacgca tttccacgcg gaaaccggat cgacaaatac
    51061 gggtacgttg gcagcattat ccagaatcca tgccagtgcc tcttcactga tattacagtc
    51121 cgcgacaatg acctttgccc tctgaataaa ttcacggtgc tgtgcgagat attcagctgt
    51181 aatagcgttg ctaatattca tgtcatttat agcaaccagc atttcaccgg tattatcgag
    51241 taatgataaa tagctcgacg tattttctcc cggcacaatc aggcatttat cgacataaac
    51301 gccagattga ttggtttgcg ttagcagcga ttggccataa aaatcactgc ctacggcgct
    51361 cagtagccag gctttattac ccagcaacgc caggttttgt gcaatattgc gccctacccc
    51421 accaggcgta aattttattt tacctggatt tgaatccgca taatttaatg attcatgtga
    51481 atatccggcg acatcaatat tcgccgagcc tataattacg acataatcct tttcgcgcat
    51541 agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc acaaggatat agatactgca
    51601 aagacggttt aaacatgtgt tcatatttaa acatgtgctc atagtatgca tgttttacgt
    51661 aaagtaaatt gccgccagag aggcaaagcg ggcagatttg agcaggctca caaaatgcga
    51721 ttaaatgcct gaattacaaa agcgaggaat ttgtgacggg atgcacagaa catgaacact
    51781 aaaaaagggc agaaaagaat ctgcccttga ggaaataacc ttagtgtttg atgaacgttt
    51841 caggtttcta ctgaaagata aaaccattta aattcataaa attacattca caaaccggtc
    51901 attctctttg cgctcatcca atgatacggg gcagtaaatt tctgccctgt aacacacctt
    51961 ttattacgcg gctttcgcta ctgcgtccac ttccggacgt ttcaggaagg cataggccaa
    52021 acccgccacc agcgtaccgg caataattgc taccaggtaa cccagtaccg gcgtaatagc
    52081 gccagggatc agcagaacaa acagaccacc gtgcggtgcc atcagtttcg caccaatcgc
    52141 cattgagatt gcgccagtca gcgccccacc aacgatacaa cacggcagca cacgcatcgg
    52201 atcacgagca gcaaaaggaa ttgcaccttc cgaaataaag cacagaccaa gaaccagagc
    52261 ggctttgcca ccttcctgct gcgctttgtc aaatttgcga cgcgccacca ttgttgccag
    52321 acccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
    52381 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttatttaccg gaccgcccat
    52441 gtcagtacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
    52501 ggtctgcagc cagtgagtca acccttcgag aatgccagca accggtttac cgatcaggta
    52561 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
    52621 cgcctccata ctctgtggca gtttcagttg cgtactgatt aacttcgcaa tgtaaccagc
    52681 caggaagccc gcaataatac cgccaatgaa gccagaaccg gtgctgaccg ccagcatacc
    52741 gccaatcaga cccggcgtga gacccggacg atcggcaatg gagaaggcaa tataacctgc
    52801 cagtaccggc accatcagcg caaaggctga accaccacca atctgcatca gcgccgctgc
    52861 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
    52921 accacctgca acgaccatcg gcagcatgta agagacgccc gtcagcaagt gacggtatgc
    52981 gcctgcactc tcgttcttac cttcagaggt cgccgtttga gctttgcccg ccggttcata
    53041 cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg gttttcttca gcgccagacc
    53101 ggtagaggtg cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
    53161 cgccacaatc accagatccg ctgcggctac ttcttcggga gtgattgcat tacccgcgcc
    53221 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
    53281 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
    53341 gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagcg accggcgcag tgtaaggttt
    53401 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
    53461 gcccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
    53521 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
    53581 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
    53641 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
    53701 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
    53761 gggctgcaac agcagtcgcc agacgcagtg tgtgttcact ggattcacgc attagcaagc
    53821 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
    53881 acggtggttt ggcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
    53941 gtgaaataac gacatgcgca ataccttgtt cacgcagcgc atgcgcagct tcaatcacat
    54001 ctttcatttc aggcagttta cggcctgccc agatttccag ctcgcggcgg ttaggtttca
    54061 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
    54121 tgcaagggca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
    54181 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
    54241 cagtcacaaa gcgttcccag tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg
    54301 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
    54361 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
    54421 taccgaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
    54481 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
    54541 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgata gtagcaacac
    54601 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccga ttgctttcag
    54661 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
    54721 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtgc catcaaggtt
    54781 tgtcacggta atatcactgt taaattgttt aatggtgttg accagcatgg tacctggacg
    54841 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
    54901 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tccgctttca gcaagcggtc
    54961 agctttattg tcgagcaaca aatcagcgag acgcttaaga accgcaatgg gctgatcgtc
    55021 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
    55081 cgcacggctt accgcaattg cgctacgcag attgccttcg gcgctatcgc tcagccagat
    55141 accctgtccg aggttcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
    55201 tgcccccgcc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
    55261 gacgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
    55321 catcagcaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
    55381 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgctcat ccgagctggc
    55441 agcaataccg atcgctacgt acgctacctg accgtcaccc caggtgacgc cttccgggaa
    55501 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
    55561 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
    55621 gacgtagcct tctgctacat taccggcctg caccagcgcc gcagcgacct ggcgaatcgc
    55681 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
    55741 agttctcctc tcttgctgaa ttgaaacgat tcagcttcta tgagaaaaaa agcgccaacc
    55801 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
    55861 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
    55921 ttgtcatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
    55981 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
    56041 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
    56101 gaccagcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
    56161 ggcgttttta atcgttgcct ttctcaccgg tattgcgggc gctctgcaaa ccccgacact
    56221 cagtattttt cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
    56281 cggcagcgct gtcattgggg ttctggtgag tcagtttctc gccgggcgct ctgataagcg
    56341 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
    56401 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
    56461 tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg
    56521 acgtgaggcg gtgatgttca gctctttttt acgcgctcag gtttcactgg catgggtcat
    56581 tggcccaccg ctggcttatg ccttagcgat gggtttcagc tttacggtaa tgtatctgag
    56641 cgcagcggta gcatttattg tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca
    56701 aaaagagctt ccgctggcga ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac
    56761 gctgctgctg tttgtcattt gtacattgat gtggggctcg aacagcctgt acatcatcaa
    56821 catgccgcta tttattatca acgaactcca tcttcccgag aaactggccg gtgtgatgat
    56881 ggggaccgcc gccgggctgg aaatcccgac catgttgatt gccggatatt tcgccaaacg
    56941 tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg
    57001 aatgctgatg gcgcattctc ctgtcattct gttgggcttg cagctgctaa atgctatttt
    57061 tattggcatt ctgggcggta tcgggatgct ctattttcag gatctgatgc ccggtcaggc
    57121 aggttcagcc accacgctct ataccaacac gtcgcgcgtg ggctggatca tcgcgggatc
    57181 agtggcgggc atcgtcgccg agatctggaa ttatcacgct gtgttctggt ttgcgatggt
    57241 gatgattatc gccactctgt tttgcttact gcggattaaa gatgtttaag gcgcggtcag
    57301 catttccaga tcgataagcc aggtcattgc ctgctgacgc gagttaccgc acatttcagc
    57361 gctggcttgt aagcctgcac agactttcgg ccgcagtggc gaggtgaaaa ttttacagcg
    57421 ttgctgttca tcaagctgaa tgcagggcgt attggcgggc ttgccatctg gcataccggg
    57481 aatagggctg gaaattgaag gggcagtgca acacgccccg caacccggac ggcattccat
    57541 acgttgttct cttaaggtta accaccggat tgatgagcgc gcacagtacc accttttttg
    57601 caccagcaaa agtgcgaata ccacttgccc gaaaggctcg tcgcgagtac tttgtcgcga
    57661 tatttttgac attttcgact acaggaattt ttcgatgcca agagcgaacg aaattaaaaa
    57721 aggtatggta ctgaattaca acggcaaact gctgctggtt aaggacattg atattcagtc
    57781 gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg
    57841 gctgaaagta gaagagcgtt tcaaaggaga tgatatcgtt gacaccgtga cgctgacccg
    57901 ccgttacgtt gacttctcct atgtcgatgg caacgaatat gtctttatgg ataaagaaga
    57961 ctataccccg tataccttca ccaaagatca gattgaagaa gagttgctgt ttatgccaga
    58021 gggcggcatg ccggacatgc aggtgctgac ctgggacggt caactgctgg cgcttgagct
    58081 gccgcagacc gttgatctgg aaatcgttga aaccgcacca ggcatcaaag gggcatctgc
    58141 cagcgcccgt aacaaaccgg cgacattgag cactggtctg gtgattcagg taccagaata
    58201 cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc
    58261 tgactaactt cagccgcatg cagaaaaggg atagctcagg ctgtcccttt tttaatttat
    58321 tataccaatc ttctattttg cgcttcctga acaccttacg ccctcaattt tcactcgttg
    58381 attgatcgcc ctcacacttc atcgcattaa caatccagac caatttcaat tgctgtcata
    58441 taactttaca ctgtcattgt taattaatcg ttactaagac atgactccta tgaatacaat
    58501 cgcctccgtt acgctcccgc atcatgtaca cgctccacgc tacgatcgcc agcagttgca
    58561 atcacgtatc gttcattttg gctttggagc ctttcaccgc gcacatcagg cgttactgac
    58621 cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc tgtgaaatca gcttgttcag
    58681 cggtgatcaa ctgatgagcc agctccgcgc acagaaccat ttatataccg tgctggagaa
    58741 aggggcggac ggcaatcagg cgataattgt cggtgccgtt cacgaatgcc tgaatgcaaa
    58801 actggattcc ttagcggcaa ttattgagaa attttgcgag ccacaggtgg caattgtttc
    58861 cctgacgatt accgaaaaag gctattgtat tgacccggcc accggtgcac tcgacaccag
    58921 caatccgcgg attattcacg atctacaaac ccctgaagaa cctcactccg caccgggtat
    58981 tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt acaccgttta ccgtgctctc
    59041 ctgcgacaat attcccgaca atggccatgt ggtgaaaaac gcggtgctgg gaatggcaga
    59101 aaaacgttcg ccagaactcg ccgggtggat aaaagaacac gtcagttttc cgggaaccat
    59161 ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg gcagaaatca gccagcatct
    59221 gggggtaaat gatccctgcg cgattagctg cgaaccgttt atccagtggg tggtggaaga
    59281 taacttcgtc gctgggcgtc ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt
    59341 cctgccatgg gaagagatga aactgcggat gcttaatggc agccactctt ttctcgctta
    59401 tctgggttac ctctcaggat tcgcccatat cagtgattgc atgcaggatc gcgcatttcg
    59461 ccatgccgcc agaacattaa tgctggatga gcaagcgccg acactgcgga ttaaagatgt
    59521 cgatttaaca caatatgcgg ataagttaat tgcacgtttt gctaatccgg cgctgaaaca
    59581 taagacctgg caaatcgcga tggatggcag ccagaaatta ccgcaacgca tgctggcagg
    59641 tattcgcata catctggggc gcgaaacgga ctggtcgttg ctggcattag gcgttgcagg
    59701 ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat gccattgatg ttcgcgatcc
    59761 gcttagcgat aaaattcgcg atcttgttgc gggcagcatc agtgagcaac gcgtcaccgc
    59821 cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca gataacccgc attttgtgca
    59881 ggccatcgaa caagcccggc aacaaatcgc acaattcggc gcgcatcagg cgctattaaa
    59941 caccctcaaa atttaacgat ttctgcggtt aaagcggatg aagctcgcct tcgtccgctc
    60001 tccccttctc ttttctgccg tttttagcca ggattaacgc tcagttaact taccagagta
    60061 ttgcggagct gttgtgacca ggaccaacct catcaccggt tttctcggca gcgggaaaac
    60121 cacgtcgatt cttcatctgt tagcccataa agatcccaac gaaaaatggg cggtactggt
    60181 taatgaattt ggggaagtcg gaattgatgg tgctttgctc gccgatagcg gcgcactgct
    60241 gaaagagatc cccggcggct gcatgtgctg cgttaatggt ttacccatgc aggtagggtt
    60301 gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg atagagccga ctgggctggg
    60361 ccatccgaaa cagatcctcg atctgttaac cgcaccagtc tatgaaccgt ggatagatct
    60421 gcgcgccacg ttgtgcattc tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa
    60481 tgaaaacttc cgtgaccagc tggctgccgc agacatcatt gtcgccaata aatccgaccg
    60541 tgcgacacct gaaagcgagc aagcgctaca gcgctggtgg cagcaaaatg gtggcgatcg
    60601 gcaattaatt cacagtgagc atgggaaagt tgccggtcat cttctggatt tgccgcgtca
    60661 caatttagcc gagttgcccg ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg
    60721 gttagcagcg ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagtggaca
    60781 aggacatcag gcctgcggct ggatattcga cgctgatacg gtattcgaca ccattggcat
    60841 tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga
    60901 agggctggtg cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc
    60961 gccaccggac agccgtattg agctgatttc cagcagcgaa gctgactgga atgccttgca
    61021 gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt
    61081 aagataatta gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc
    61141 ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccgatt
    61201 gatgcggata ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg
    61261 ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt cactgctggc aatgggaatg
    61321 ttgatgctga gtttctggct gaaagaaaac gccccaggca gacgacgtat cgtgattatt
    61381 ggtctggtca tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct
    61441 gtaaaacggg ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc
    61501 tctgtcccca cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg
    61561 cttatttttt cggcattcat gtggcgttat ttcggcaaag ttgcaggtct tatcgccctt
    61621 attatttttg tggtttttgc gtttcccaga gtaatgattg gcgcacactg gtttactgac
    61681 atcattgtcg gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca
    61741 ttaagtgatc gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc
    61801 caaaacaaat aactcacagt acttaacatc atcaggttta tttttatagt gaggataatc
    61861 ctgatgatgc gcaccgtgct ttcatccatc gaaagcaaaa atcattctct aagtaaatga
    61921 atggattgca tgcatttcac tcaatcgtac tttaattgac caatcccgct tattaacttt
    61981 ctgtatcact ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg
    62041 ggtaatctcg aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg
    62101 cgttagtcca cagatttggc cttaaggaat tgtttcaaca tgccctggta attagtctcg
    62161 tgtcgcttgg catttttttt ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa
    62221 caacatggtc aaatctcaac cgattttgag atatatcttg cgcgggatcc ccgcgattgc
    62281 agtagcggtt ctgctttctg catgtagtgc aaataacacc gcaaagaata tgcatcctga
    62341 gacacgtgca gtgggtagtg aaacatcatc actgcaagct tctcaggatg aatttgaaaa
    62401 cctggttcgt aatgtcgacg taaaatcgcg aattatggat cagtatgctg actggaaagg
    62461 cgtacgttat cgtctgggcg gcagcactaa aaaaggtatc gattgttctg gtttcgtaca
    62521 gcgtacattc cgtgagcaat ttggcttaga acttccgcgt tcgacttacg aacagcagga
    62581 aatgggtaaa tctgtttccc gcagtaattt gcgtacgggt gatttagttc tgttccgtgc
    62641 cggttcaacg ggacgccatg tcggtattta tatcggcaac aatcagtttg tccatgcttc
    62701 caccagcagt ggtgttatta tttccagcat gaatgaaccg tactggaaga agcgttacaa
    62761 cgaagcacgc cgggttctca gccgcagcta ataaaccgtt tggatgcaat cccttggcta
    62821 tcctgacgag ttaactgaaa gcactgttta ggcagtgctt ttttgttttc attcatcaga
    62881 gaaaatgatg tttccgcgtc ttgatccagg ctatagtccg gtcattgtta tcttttaaat
    62941 gttgtcgtaa tttcaggaaa ttaacggaat catgttcata cgcgctccca attctggacg
    63001 taagctcctg cttacctgca ttgttgcagg cgtaatgatt gcgatactgg tgagctgcct
    63061 tcagttttta gtggcctggc ataagcacga agtcaaatac gacacactga ttaccgacgt
    63121 acaaaagtat ctcgatacct attttgccga cctgaaatcc actactgacc ggctccagcc
    63181 gctgacctta gatacatgca agcaagctaa ccccgaactg accgcccgcg cggcgtttag
    63241 catgaatgtc cgaacgtttg tgctggtgaa agataaaaaa acattctgtt catctgcgac
    63301 cggtgagatg gacattccac tcaatgaatt gattcctgcg ctcgacatta ataaaaatgt
    63361 cgatatggcg atcttacccg gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg
    63421 gtatcgcaac cctttgctga aaaatagcgg cgtctttgcc gctctgaatc tcaacctgac
    63481 gccttctctc ttttatagct cacggcagga agattacgat ggcgtcgccc tcattattgg
    63541 caatactgcg ctatctacct tttcttcacg tttgatgaac gttaacgaat taaccgacat
    63601 gccagtccgt gaaactaaaa ttgcgggcat tcctctgacc gttcggcttt atgcagatga
    63661 ctggacatgg aacgatgtgt ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt
    63721 tggcctgctc tgctattacc tgatgagcgt acgtatgcgc cccggcagag aaatcatgac
    63781 cgccatcaag cgcgaacaat tttacgtggc gtatcaaccg gtggtggata cacaagcttt
    63841 gcgagtaacg ggcctggaag tactgctacg ctggcggcat cctgtcgcgg gagaaattcc
    63901 cccggatgcc ttcattaact ttgccgaatc gcaaaagatg attgtgccgc tgactcagca
    63961 cctgtttgag ttaattgccc gcgatgccgc agaattagaa aaagtgctgc cggtaggcgt
    64021 caaatttggc attaacattg cgccggacca tctgcacagc gaaagcttta aagcaaatat
    64081 ccagaaactg ctcacttccc tacccgcaca ccatttccag attgtgctgg aaattaccga
    64141 gcgcgatatg ctgaaagagc aagaagccac acaactcttc gcctggctgc actcggtcgg
    64201 cgtagaaatt gctattgatg acttcggcac cgggcacagc gcgcttatct atcttgagcg
    64261 ttttacgctc gattatctga aaattgaccg tggatttatc aacgccatcg gtacggaaac
    64321 gatcacttca cccgtacttg acgcggtgct gacgctggcg aaacgcctca atatgctgac
    64381 ggttgctgag ggggtcgaaa cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa
    64441 tttcatgcaa ggctactgga ttagtcgccc gttaccgctg gacgattttg tccgctggct
    64501 gaagaaaccg tatacgccgc agtggtaagg tgtgcttacg tcccttatta ttcatagtga
    64561 aagcatgccg gattgcggct aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg
    64621 catactgctg ctgtttatcg ctctgttcac ctttggtgcg caggcgcagg ctatcaagga
    64681 aagctatgcc tttgccgtgc tgggcgaacc ccggtacgcg tttaatttca accattttga
    64741 ttatgtgaac cccgccgcgc caaaaggtgg gcagataacg ttgtcagccc tcggtacctt
    64801 cgataatttc aaccgctatg cactgcgcgg caacccgggc gcacgcaccg agcagctgta
    64861 cgacacgcta tttacgactt ccgatgacga accaggcagt tattacccgc tgattgctga
    64921 aagcgcacgc tatgctgacg attattcctg ggtggaggtc gctattaatc ctcgcgcccg
    64981 ttttcatgat ggttcgccca ttactgcccg cgatgtagag tttacttttc aaaaatttat
    65041 gactgaaggc gtgccgcaat ttcgtctggt ctacaaaggc accaccgtca aagccattgc
    65101 gccgttaacc gtgcgcattg agttagctaa acccggcaaa gaagatatgt tgagtctgtt
    65161 ttcgctgccg gtatttccag aaaagtactg gaaggatcac aaacttagcg atccgctcgc
    65221 cacgcctccg cttgccagtg gtccgtaccg aattacgtcc tggaaaatgg ggcaaaatat
    65281 tgtctattcc cgcgtgaaag attactgggc agcaaactta ccggtaaacc gtggacgctg
    65341 gaatttcgac accattcgct acgattatta cctcgatgat aatgtcgcct ttgaagcgtt
    65401 taaagcaggt gcctttgatt tgcgtatgga aaacgacgcc aaaaactggg ccacacgcta
    65461 tagcggtaaa aatttcgata aaaaatacat catcaaagat gagcaaaaga acgaatcagc
    65521 ccaggatacg cgctggctgg cgtttaatat ccaacgtccg gtattcagcg atcgccgggt
    65581 ccgggaagca atcaccctcg cctttgactt tgaatggatg aacaaggcgt tgttttacaa
    65641 tgcctggagt cgcacgaaca gttattttca gaataccgaa tacgcggcca gaaattaccc
    65701 cgacgccgcg gagctggtgc ttctggcacc aatgaaaaaa gatttaccgc cagaagtctt
    65761 cacacaaatc taccagccgc cggtatcgaa aggcgatggc tacgatcgtg acaacctgtt
    65821 aaaagccgac aaacttctca acgaagcggg ctgggtgctg aaaggtcagc aacgcgttaa
    65881 tgccactacg ggtcagccgc tcagctttga attattgctt cctgcaagca gcaatagtca
    65941 gtgggtattg ccgttccagc acagcctgca acggctgggt atcaacatgg acattcgcaa
    66001 ggtggatcac tcgcaaatca ccaaccgcat gcgcagtcgc gactatgaca tgatgccgcg
    66061 cctatggcgg gcgatgccgt ggcccagttc cgatttacag atttcctggt catcggaata
    66121 tatcaattcc acttataatg cccccggcgt gcaaagcccg gttatcgact cgctgatcaa
    66181 ccaaattatt gccgcgcagg gaaataaaga aaaattactg ccgttagggc gagcactgga
    66241 tcgcgtatta acgtggaatt attacatgct gccaatgtgg tacatggcgg aagaccgtct
    66301 cgcctggtgg gataaattct cccaacccgc tgtgcgccct gtttacagtc tgggtatcga
    66361 tacctggtgg tatgatgtta ataaagcggc caaactgccg tcagccagac aacagggaga
    66421 gtagatgggc gcttatctga ttcgccgtct gttgctggtg atcccaacgt tatgggcgat
    66481 tatcactatc aactttttca tcgtgcaaat tgcgcctggc ggtccggtcg accaggccat
    66541 cgccgccatt gagtttggta atgccggagt attacccggc gcaggcggtg aaggcgttcg
    66601 cgccagccat gcgcaaacgg gcgtcggtaa tatcagcgac agtaattacc gtggcggacg
    66661 cggattagat ccagaagtga tcgccgagat cactcatcgc tacggtttcg ataagccgat
    66721 ccacgaacgt tacttcaaaa tgctctggga ctacatccgc tttgattttg gcgatagcct
    66781 gtttcgcagc gcctcggtgc tgacgctgat taaagacagt ctgccggttt ccatcaccct
    66841 cggattgtgg agcacgctga ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc
    66901 tgtttataat gggagccgct ttgacgtctg gagtagcgca tttatcatca tcggctacgc
    66961 cattccggcc tttttgtttg ccatcctgct gattgtcttc ttcgcaggcg gcagctattt
    67021 cgacctgttc cctctacgcg gcctggtttc cgctaacttt gattcgctgc cgtggtatca
    67081 gaaaatcacc gattatctgt ggcatatcac gctgccggtg ctggcgacgg tgattggtgg
    67141 ctttgcggcg ctgactatgc tgacaaaaaa ctcattcctt gatgaagtac gcaagcaata
    67201 cgtggtgacc gcgcgtgcga aaggggtaag tgaaaaaaat attctctgga aacatgtgtt
    67261 ccgcaacgcc atgctgctgg tgattgccgg ttttccggcg acgtttatca gcatgttttt
    67321 taccggctcg ctgctgattg aggtgatgtt ttcactcaat ggtcttggct tactgggcta
    67381 cgaagcgacc gtctcgcgcg attatcctgt aatgtttggt accttgtata ttttcaccct
    67441 gattggcctg ctgctgaata ttgtcagtga tatcagctat acgctggtcg atccgcgtat
    67501 agattttgag ggacgttaat gtcgcgactc aaccccgtca atcaggcccg ttgggcgcgt
    67561 tttcgccata accgtcgcgg ctactggtcg ttatggattt tcctcgtttt gtttggtttg
    67621 agtttgtgtt ctgaacttat cgccaacgat aaaccgttgc tggtgcgtta tgacggcagt
    67681 tggtatttcc cgttgttgaa aaactacagc gaaagcgatt ttggcggccc gctggcaagt
    67741 caggctgatt atcaggaccc gtggctgaaa caacggctgg aaaataatgg ctgggtactg
    67801 tgggcaccga ttcgctttgg tgctaccagt atcaactttg ctaccgataa gcccttccct
    67861 tctcccccct cccggcaaaa ctggctggga acggatgcca acggcggcga tgtgctggca
    67921 cgtattctct atggcacgcg gatctcggtt ctgtttggcc tgatgctgac tctctgttcc
    67981 agcgtgatgg gcgtgctggc gggggcgcta caaggctatt acggcggtaa ggtcgatctc
    68041 tgggggcagc gctttattga agtatggtcg gggatgccaa cgctgttttt gattattctg
    68101 ctttccagcg ttgtgcaacc taacttctgg tggctgctgg caattactgt cttatttggc
    68161 tggatgagtc tggtcggcgt ggtgcgggcg gagtttttac gtacccgcaa tttcgactac
    68221 atccgcgcgg cacaggcgct tggcgtcagc gatcgcagta ttattctgcg tcatatgttg
    68281 ccgaatgcaa tggtcgctac cctcaccttt ttaccgttta ttttatgtag ttcgatcacc
    68341 accctgacct cactcgattt cctcggcttc ggtctgccgc tcggttcacc gtcactcggt
    68401 gaactgctgt tacaagggaa aaataacctt caggctccgt ggcttgggat caccgccttc
    68461 ttgtcggtgg cgatattatt gtctttgctg atatttattg gtgaagccgt ccgcgacgca
    68521 tttgatccta ataaggcggt gtagcatgac gcaaactctg ttagcgattg aagatttgtc
    68581 ggtgggtttt cgccatcagc aaaccgtacg tacagtagtc aatgatgttt cactacagat
    68641 tgaggctggc gaaacgctgg cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc
    68701 gctgtcaatt ttacgcctgc tcccttcccc gccggttgaa tatctctccg gcgatattcg
    68761 ttttcatggc gaatcgctgc ttcatgccag cgaccaaacg ttgcgcggtg tacgcggtaa
    68821 taagatcgcc atgatttttc aagagccgat ggtgtcgtta aatccattgc ataccctgga
    68881 aaaacagctt tatgaagtgc tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg
    68941 cgaaattctt aactgccttg atcgcgttgg tatccgccag gcggcaaaac ggctgacaga
    69001 ttatccgcat cagctctccg gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt
    69061 aacgcgaccg gaattattaa ttgccgatga accgaccacc gcactggacg tctctgtcca
    69121 ggcgcagatt ttacagctgt tgcgcgaact gcaaggcgag ttgaatatgg gcatgctgtt
    69181 tattactcat aacctcagca ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa
    69241 cggtcgctgt gtcgagcaaa attacgccgc tacgctattt gcctcaccca ctcatcctta
    69301 cacacaaaag ctactcaaca gtgaaccgtc aggcgatcca gtgccgttgc cagaacctgc
    69361 ctcaacgttg ctggatgttg aacagcttca ggttgccttc cccattcgca aagggatttt
    69421 gaagcgcatt gtggatcata atgtggtggt gaaaaacatc agttttacgc tacgagcggg
    69481 tgaaacactg ggtttagtgg gcgagtccgg ttccgggaaa agcacgacgg gactggcgct
    69541 gctgcgactg attaattctc agggcagcat catctttgac ggtcagccac tgcaaaattt
    69601 aaatcgccgc cagctgttac ctattcgtca tcgcattcag gtggtatttc aggatccaaa
    69661 ctcctcgctc aacccacgac tcaacgtttt gcagattatt gaggaaggct tacgggttca
    69721 ccagccgacg ctttctgccg cacaacgcga acaacaagtg atagacgtga tgcatgaagt
    69781 gggattagat cctgaaacac gccaccgtta tccggcggag ttctctggtg gtcagcgaca
    69841 acgtattgcg attgccaggg cattaattct taagccctcg ctgatcatac ttgatgaacc
    69901 gacatcatca ctcgacaaaa cggtacaggc gcaaatattg acgctattga aatcattgca
    69961 acaaaagcat caactggcct atttgtttat cagccacgat ttgcacgttg tccgcgcgtt
    70021 atgtcatcag gttatcgtac tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg
    70081 cgtgtttgcc acaccgcagc aggagtatac gcgtcagcta ctggcgttga gctgacgctt
    70141 aaaaaggatt gtagtttgaa aagggttcgg caattgccac accaaaattt ttcaatcggc
    70201 atgttgcagc aaactcgtcc tggcgattta caaacaggca cggctcacct tcacactcca
    70261 gcaccgaaca tggaacttcg atgtcgccta acgcctggct tagtttatac atcaccgacc
    70321 atgcattatc accatccggg ctaagcaatt taagcgccac caggctgttg tcaccgcacg
    70381 gtccattttg cggaatcggt tcaaccttcg aacctgcgaa accagcagac cagcgatagt
    70441 tctgcggcag tcgatggacg attgagagtt gtaatgaagt cacttatttt ccccggaagc
    70501 acatttactt cacaatttgt ttacatcaat tttaacacat catcaacaat ccgtctttat
    70561 acagatcgta aagattgatg cctcgcctgc gtggcctcta tggctctgat ttaagtaata
    70621 tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg tttctgcgat ctaactcaac
    70681 ctttttaact acaatgatgt gactttttac ataaattgat tttacataaa ataaacatat
    70741 atcgggggaa tgatagattt gtggttgaca tgcatcaaca aaggaagcct tttagcttcc
    70801 tcgttgtgca atagatcacc gttttttcgg ccgactggcg tacagacaga agagaatgga
    70861 gctggttgcg cagaatgcaa ttgaccaaat catcggccat gcagagttaa aggtcgcaag
    70921 agaaagcaat gcgccaacaa ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga
    70981 tgccgttccc gccatatggg gaaactcatc aagaatgacc gccatcgcat tggatgacac
    71041 catcgacacg cagcccacaa acgccgcaac gccaaccacc agcgaccaaa atcccagacc
    71101 cagcagcgca ctgatgacca tccacgctgc cataataaat tgtatccaca accccgagcg
    71161 gaacatattt aacgcgccaa tacggcggac gaagcggcta ttaaagatgg tcatcacgaa
    71221 caggaaaaca atgtttagtg caaagtaata accaaagttt tccggcgcga cgtggttaat
    71281 ttcaatgtag acaaacggtc cggcgcttaa gaacgagaac atcccggcaa agctgaaacc
    71341 actggccagc atataactca ggacacgctt atgacggaac agcgccgcaa agttaccaat
    71401 agtggtacga atgtgaaaag gctggcgacg ctccggtggt aaggtttctt tgatcagaaa
    71461 gaagatcatt gccgaagcca gaatcgccgc cacggcgagg atccagaaga tgtaatgcca
    71521 gctcagccac accagcaccc agccgccaac tatcggtgcc atcagcggtg caatggtcgt
    71581 caccagcatg acaaacgaca tcatccgcga gaactcttct ttcgggtaga tatcacgcat
    71641 caaagcgttg ataaccacgc tggccgcagc cgccgccagc ccgtggaaga aacgcatcac
    71701 aatcagctga tcgatggttt gtgccaacgc acacgccacc gcggcggcgg caaacaccag
    71761 cgtaccgcca agaaccaccg gcttacgccc gaagctgtct gccatcggcc cgtagattaa
    71821 ctgccccaac gcaaagccca gaatataagt actgagggtc atctgcgtac tgcccgccgg
    71881 tacgccaaac tgcgctgaaa ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg
    71941 catcaacatg gccagcaggc caaggataaa aacaatagca aacgacgaat gctgtcgggt
    72001 ggtcacaacg ggctcctgaa agtcattgaa aagttagacg acgctggcaa tttcttcttc
    72061 agttaacgga cgatattcac cgggggctaa atcagcatcc agcgtaatgc cgccaatacg
    72121 ttcacgatgc agctcaacca catggttacc cacggcggcg aacatgcgtt tcacctgatg
    72181 ataacgccct tcgctgatgg tcagacgaac ctgcgttggg gtaatcactt caagcaccgc
    72241 tggcttagtg agatcttttt cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt
    72301 atcgtcagct acgggtgatt ccagcgtcac cagataggtc ttctcgcaat gatggcgcgg
    72361 agaagtaatg cggtgcgacc actgaccatc atctgtcatt agtaccagac cggtggtatc
    72421 gatatccagc cgccctgcgg cgtgcagttt ccacgccact ggttcatcta gaaaatagag
    72481 caccgttggg tgatcagggt cgtccgtgga gcaaacatag ccctgaggct tattgagcat
    72541 gaagtaacgt ggaccgtgtt gctgcgccag cgggttgcca tcgtaagcga catcatgttc
    72601 aggaagcagt ttgaacgctg cattacggac gatttcgcca tcgacggtga cacgattgcc
    72661 gcggatttca cgcccggcaa tagcacggct aacgccgagt tgctgtgcga taaatttatc
    72721 aagtcgcatg tgtgtgattt tgcctgtaaa aacggaggtc gggcattgac ccgaaaatct
    72781 gaactgttgt ctgcccagta tagcggtcta ataacttccc tcaagggaaa aagaaccgtg
    72841 gcatactatt agcagaataa tctacctacg cgagaccatg atttttacac tccgcccata
    72901 tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt
    72961 tatcgtgctg cccaccggtg caggtaaaag cctggtgata gcggaacttg cgcggctggc
    73021 tcgtggtcgc gtgctggtgc tggcacacgt taaagaactg gtggcgcaaa accatgcaaa
    73081 gtatcaggcg ctggggctgg aagccgatat ttttgccgcc gggctaaagc gcaaagagag
    73141 ccacggtaaa gtggtatttg gcagcgtgca gtcggtcgcc cgtaatcttg atgcctttca
    73201 gggtgaattt tcgctgttga ttgtcgatga atgtcaccgt attggtgacg atgaagagag
    73261 ccagtatcag caaatcctca ctcacctgac aaaagtgaat ccccacttac gcctgctggg
    73321 gctgactgcc acgccttttc gactgggcaa aggctggatc taccagttcc attatcacgg
    73381 catggtacgc ggcgatgaga aagccctttt ccgtgactgc atttatgaac tgccgctgcg
    73441 ttatatgatt aaacacggct atctgacgcc gccagaacga ctggatatgc cagtagtgca
    73501 atacgatttc agccgcttgc aggcacagag taacgggcta ttcagcgaag ccgatctcaa
    73561 ccgcgagcta aaaaaacaac aacgtattac cccgcacatc atcagccaga ttatggagtt
    73621 tgctgcaacg cgcaaagggg tgatgatttt cgccgccacc gttgaacacg caaaagagat
    73681 tgtgggatta ctacccgccg aagatgctgc actgattact ggcgacaccc ccggcgctga
    73741 gcgcgatgtg ttaattgaag attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc
    73801 ggtactgacc accggatttg acgccccgca cgtcgatctt atcgccattc tgcgccctac
    73861 cgaatcagtg agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg ctcctggcaa
    73921 gactgattgc ttaattcttg attatgcggg taatcctcac gatctctacg cgccggaagt
    73981 tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg
    74041 ttttgccaac accttttggg ggaaaacgac cgccgacggg acattgattg aacactttgg
    74101 tcgccgctgt cagggatggt ttgaagatga cgacggtcat cgcgaacagt gtgacttccg
    74161 tttccgtttt aaaaattgcc cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg
    74221 ccgcgaatgt gacacggtac tggttgatcc ggatgatatg ttaaaagcgg cgctacgact
    74281 gaaagatgcg ctggtattac gctgtagcgg catgtctttg cagcatgggc acgacgagaa
    74341 aggcgaatgg ttgaaaatca cctattacga tgaagacggc gcggatgtga gtgagcgttt
    74401 ccgtctgcaa acgcccgccc agcgaactgc cttcgagcag ctttttatcc gcccgcatac
    74461 gcgcacaccg ggcatccctc tgcgctggat caccgccgcc gatatcctcg cccagcaagc
    74521 cttattgcga cacccggatt ttgtcgttgc ccgcatgaaa ggtcagtact ggcaagtgcg
    74581 tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg
    74641 gtagttttga ttgatgtata aggcgattga gtatagaatc tcgcccgctt ttgcatacgc
    74701 aaagcagatc acttacctgt tgctgggtcg cctgtagcag gattaattta agagagaaag
    74761 aaatgtttac tatcaacgca gaagtacgta aagagcaggg taagggtgcg agccgccgcc
    74821 tgcgtgccgc taacaagttc ccggcaatca tctacggtgg caaagaagca ccgctggcta
    74881 tcgagctgga tcacgacaaa gtcatgaaca tgcaagctaa agctgaattc tacagcgaag
    74941 ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc
    75001 acccgtacaa accgaagctg cagcacatcg acttcgttcg cgcttaattg ctgaataagt
    75061 tgtaaaaaac cccgctccgg cggggttttt tgtatctgca gattatgcct gattacgata
    75121 ttgctatttt ttgcaggcca gataaggcgt tcacgccgca tctggcattc ataacacaaa
    75181 accgattaat tgccgccgga cgtccggcgc tgcaattgat cgcgcaaatt cggcggcgtg
    75241 cctttaatag tcagggtatc ggtcgccgga tcccagaaaa tccgttcgcc caacagcatc
    75301 gcatcaaaat taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt
    75361 gtgctacggt ctgccgggaa gctctcttcc agttcgtagc ctttttcagc cgcaaactcc
    75421 gtgaagctca cttcgctgac gcctgccagc tctttcgaca gcgattccag ttcgatctct
    75481 tcgcctgcct gcagctgctc attacagtag ctataaacct gctggcgcac gttctgtcgc
    75541 tctgctttat ccagttgtgc ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg
    75601 ttttgcgctt tcgcgttcaa tccttcgctg gccccgagga aatccataaa gaaatccgcc
    75661 actttgcgcc ctactcgccc tttcaggaaa gtgagatagc gggtcgactc tggattggtt
    75721 tcccattcgg ttaaatcgat acgcgcaaca atatccgcat gattgatatc aagataatgg
    75781 gttgggttga tgtcgagatt ttcgttaacg cgcatactgc tcaggttgct caggactgcc
    75841 accagcagat actccaccgc cagataacgg tagtggcaaa acagcacgaa tccaccgtca
    75901 gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc
    75961 agaaagtcct cttcaccctg acgctgtaag cgcagcgtct gcgccagttc actctcttcg
    76021 ctaaacaagc cgtaagcttt atttttggcg ctatagaccc gatgcagttc tgctaccatc
    76081 tcaacgacgg tttctgtcgg ttccagcaat gaatcgcgca acaccagctc aaggttctgc
    76141 tcatcacgct tgataagctg gtgcagagca atctggttga tatccagact catgataaac
    76201 tctcctttaa gaccgggcgg tattcaacca ccgccggtta agcgacgcaa taaaagatgg
    76261 cggtcatttt acagaaggcg gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa
    76321 aaaccgttgc tacggtaata tgttgccctt tcatcaacta actgatttcg atttatgcca
    76381 caaatttccc gctacagtga tgaacaggtt gaacaattgc tcgcagagct gctcaacgta
    76441 ctggaaaaac ataaggctcc gaccgacctt tccctgatgg tgttaggcaa catggtcact
    76501 aaccttatca acaccagcat tgccccggcc caacgccagg caattgccaa ctcttttgcc
    76561 cgcgccttac agtcctctat caacgaagac aaagcgcact aagggaaaca gataacaggt
    76621 tatggtaact catcgtcagc gctaccgtga aaaagtctcc cagatggtca gttgggggca
    76681 ctggtttgca ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt acctgtttat
    76741 cgccgactgg ccgaccacgc ttgctggtcg catttattcc tacgtaagca ttatcggcca
    76801 tttcagcttc ctggtgttcg ccacctactt gctgattctc ttcccgctga cctttatcgt
    76861 cggctcccag aggctgatga ggtttttgtc cgtcattctg gcaacggcgg gaatgacgct
    76921 attactgatc gatagcgaag tctttactcg tttccatctc catcttaatc ccatcgtctg
    76981 gcaactggtt attaacccag acgaaaatga gatggcgcgc gactggcagc tgatgttcat
    77041 cagcgtgccg gttattttat tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct
    77101 gcgcagcctg acgcgtcgtc ggcgcttcgc gcgtccgctg gccgcattct tatttatcgc
    77161 ctttatcgcc tcgcatgtgg tgtatatctg ggccgatgcc aacttctatc gccctatcac
    77221 catgcagcgc gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt ttcttgagaa
    77281 gcatggcctg cttgatgcgc aggagtatca acgccgtctt attgagcaag gtaatccaga
    77341 cgccgtttcc gttcagtatc cgttaagcga actgcgctat cgcgatatgg gcaccgggca
    77401 gaatgtcttg ttgattactg tcgatggcct gaactactca cgcttcgaga agcagatgcc
    77461 tgcgctggca ggttttgctg agcaaaatat ttcgttcacg cgccatatga gctccggcaa
    77521 cactacagac aacggcatct ttggcctgtt ctatggcatc tcgccgagct atatggacgg
    77581 cattctgtcg acccgtacgc ctgcggcatt aattactgcg cttaatcagc aaggctatca
    77641 gctggggtta ttctcatcag atggctttac cagcccgctg tatcgccagg cattgttgtc
    77701 agatttctcg atgccgagcg tacgcaccca atccgacgag cagaccgcca cgcagtggat
    77761 caactggctg ggacgctacg cacaagaaga taaccgctgg ttctcgtggg tctctttcaa
    77821 tggtactaac attgacgaca gcaatcagca ggcatttgca cggaaatata gccgggcggc
    77881 aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt ctggcaaact
    77941 ggacaatacg gtggtgatta tcactgccgg tcggggtatt ccactgagcg aagaggaaga
    78001 aacctttgac tggtcccacg gtcatctgca ggtgccatta gtgattcact ggccaggcac
    78061 gccggcgcag cgtattaatg cgctgactga tcataccgat ctgatgacga cgctgatgca
    78121 acgcctgcta catgtcagca cacctgccag cgaatattcg caaggtcagg atttgttcaa
    78181 ccctcaacgc cgtcattact gggttaccgc agcggataac gatacgctgg caattaccac
    78241 cccgaaaaag acgctggtgc tgaacaataa cggtaaatac cgcacttaca acttacgtgg
    78301 tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta ttgcaagtac tgacagacga
    78361 gaagcgtttt atcgctaact gattaattat aaatcagtta gcgaaatatc ttacttgcaa
    78421 tcggtgtgga aaacggtagt attaacaacc acgagtcggc acgtagcgca gcctggtagc
    78481 gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc
    78541 caaaaaaacc aaccgtaaag gttggttttt ttatatctgc atttaattcg ataaacagac
    78601 cgcgacacat cagggcctgt ttattgtctg ttatcagaac gtccagacca cacccgcctg
    78661 ggtattccac ggcgattcta cccctgcgcc attgccatag ctgactgaca aatgaccgct
    78721 taacgtcggg gtaaacgatg accttatccc agcctgataa acgccacggg ttccagccac
    78781 atcattatta aatttgccat cgtcattaac tttcacctgg ttagaatcgg cgtattcctg
    78841 acgcacggcg gctttcagcc agggttccag cgtcgtaccg ttttgcaggt ccatgtgata
    78901 gcttaccgcc gttcccgctt cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat
    78961 gccgtttgat aacgtgtagt cctgaccatc tgtggtaaag ccggtaaagg ccagataggg
    79021 tctaacactc cacaatccgt caacccaacg gaacccgctc tcgacatgag cacccgcgcc
    79081 gttactattg taatcgccaa acgctgttgc cccattactc atcttgccat ggatggtgtt
    79141 ggcaaaacgg tcaactttca ccactccatc aacataggca ccgttctgat gctcccaacc
    79201 ggcataagcc cccagggtat agctatcgac attgcctttg ccgccgcgat caaaaccaat
    79261 atcagaatga gagtaaccaa agaacaagcc gcgaattgtg ctgctttctt cacgggagaa
    79321 acggctatcg ataccgagcg tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc
    79381 agtggtcacg ttgttgcggg tgttaattgc cgaactccac atcgccgtat cgtaactaac
    79441 gccttttacg ctaccaagac gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg
    79501 ttgtgcggcc gccatattca gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc
    79561 tgccagactc cagctatggt taccattatc cagcaaggta tattcatacg taccgatatc
    79621 aacaacgcct ccggcattgc ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag
    79681 tgtaaggcta tctcctgctg ccgggctggc accggtgtcc gtcacgaata ttttgaaatc
    79741 acctgttgcc tgaccggtga cgttgagctg atcgctctga tgattcgcca tatcggtacg
    79801 catataaaaa ttgccgtttc ccgacagggt attggtggtc aacgtagtga aattttccgc
    79861 acttgccgtc acagatgaac gagtcactga ctcatccatc ataatcacat ttgcgccaga
    79921 catcgcaagg ttggctaagt taaggcgggt gttttggttc gtcgtagtga tatcggcaat
    79981 gatggtccat gcgctatttt cgagcgaaac atccgctttg ctggtatcca cggcaccatg
    80041 cgttcttaaa gaagcgccct cagaaaccac taccttaccc accaacattg cgggtgcata
    80101 atttatttgc ggcttcataa cctcaagaat gccatcattt cctctgactg acacgttaac
    80161 cattgtgcca gcccagacgt tagcgcggcc attgttgata accatatttt cagagatggc
    80221 tttagaggaa tacgttatac ttccattctg atctgatcga ccgagatcat ataccgcatt
    80281 tgcctgcact ctcgtaccag tatcctttcc cagcgattgc atagtggcat gctcatcgag
    80341 gatagtgtca atggcctgcg tgtcctccat aacaataagc ccggaaccat catccagttc
    80401 ataatttttt gacacaccat cttttatact aaattggcct ttactgtttg taccactcac
    80461 ttccagcgca ttcgttgaga caattaattt tccgcctgct tttttatcaa cgccagttgc
    80521 agtcccgcca tccataacct ccagtaagcc gccactatct acagtagtat tataagcgaa
    80581 gtcattttct tcaactcgca aacttccgcc gttttccaac agcatattgt tagcaatacc
    80641 attcttgatt tcgaactgac cgagacggtt tgttccgaat acctccatga cccgcgtgct
    80701 tgctttaata gcaccgcctg ctttctgatc taccgcaatc gctaatcccc cctctttaac
    80761 ctccagtaat cctccctttt cgatagcggc aaggacggta atcccatctt tttcaacgat
    80821 taagcgcccg cctttaatcg tgggatcatt cgctgcgcca taaacctgta gcacaccatt
    80881 attgagcgta gtctggtttg ccgcgccacc catttgaaca atttgataac catcatttaa
    80941 tactgagtat tctgcggtgc cattcatttc aatgtactga tacccgccgt taatttcagt
    81001 attactactt ataccaaatt ctttaatatg ctgttctccg ccaggattga ttttcgtatt
    81061 attggcggtc ccataaacca gctggatatc tttttccagg acaacaccat caactgtctc
    81121 accatactca actgttgatg ccaatgctat gggtgaatat aacgctgtag ctaccagaac
    81181 agatattgcg gaacgacaaa gagaaacaga accagattga tgcattgagc tttcatccta
    81241 tgaaattaat tgcagttaaa agcattgggt acagaaaata cccatagctc catacccgga
    81301 gtcagttttt aaaaactgtt taaagaaatg cacaagtatt gtgattgatt ttttagttgt
    81361 ttttcttgat gagaagctga tgcaaaattc cgtctttata atgaaaatga tgccaaagcg
    81421 aacgacaagg ttgtagtttt cgctacatgt ccatacataa aatggggtaa cattcacgcg
    81481 cctggtagcg ttaccaacgc tacgctcaaa cataatgatt ctaataaaac ctcaggagac
    81541 tactatgcct gaagcaacgc cttttcaggt gatgattgtg gatgatcatc cacttatgcg
    81601 acgcggtgtt cgtcagttac tggagcttga tcctggcttt gaagtggtcg ccgaagcggg
    81661 cgacggcgcg agtgctatcg atctggcgaa tagactggat atcgacgtga tcttgctgga
    81721 tctcaatatg aaaggtatga gtggcctgga taccctcaat gccttgcgca gggatggcgt
    81781 taccgcgcaa attattatcc tgaccgtatc agatgcctcc agcgatgtct ttgcactgat
    81841 agacgcaggc gcagacggtt atctgttgaa agacagcgac ccggaagtgt tgctggaagc
    81901 gattcgtgcc ggagcgaaag gcagcaaagt ctttagcgaa cgcgtcaatc agtacttaca
    81961 tgaacgtgaa atgtttggcg cggaagaaga tcccttcagc gtgctgacgg agcgcgagct
    82021 ggatgttctg cacgagctgg cacaggggct gtcaaataaa cagattgcct cggtgttgaa
    82081 tatttccgag cagacagtaa aagtacatat tcgcaatcta ctgcgcaaac tcaatgtccg
    82141 ctcacgcgtg gcagccacca ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc
    82201 cgatggcatc accatcgggc ctctttttat ttactctcct gcggcgacaa atgttgcatc
    82261 gcctgcgcga tactacgttc aatcaccgca cggcgagtat cgttggcagg taagagtttc
    82321 aacatcatct cccacgcggc aacggcttcg ccaaatcgct gctgctcaaa ggcattaaac
    82381 gcatacatgc ttaacacacg aatattgcta tggtccgttc tcaccaactg gcgcagcagt
    82441 tcgccgccga ggcggttgtc gttgggatca gatgaacgtg tcaacgcttc agcataaccc
    82501 agtgtggcat cgctgttttt cggatcgagg cgatacgcag tagcgtatgc atcggtggcg
    82561 atactggcgt tacccagcgc catgccaacg cgacccaaca taatccaacc ttctatatct
    82621 cccggatttt tttgcagttg agtacgcatc cccagcgcaa ggcgcgacat ctcttcttcg
    82681 ttgagcggat cggctttcgg atccagcgcc ctgtccagca acgccggagc ctgtgccgtg
    82741 gcctgctgcc agattttcac ctgctgataa ttgccagtct ggtagtagct gacgccagcc
    82801 acaattaacg ccaccacaat acccggcaga taaacaacat atccggcacg cttaccttcc
    82861 ggcacgcttt gttcaggaaa cgcttccggt accacgcgta cccgacgccg cgaacgggcg
    82921 tatatgaccc aaccgccaat accaatagcc actaccggaa gcacccacag cagcacggtc
    82981 agcggcgtta acggcggatc gtaagtgacg aagttgccgt aacgcgccac catataatcg
    83041 acaatctctt tcttactttt gccttcctgc atcagttcat acactttctg gcgcaggtcg
    83101 gtggcaatca tcgagttgga atcggcaatg ctgttattct ggcatttcgg gcagcgcagt
    83161 tcttcagtga gctgacggaa ctgttgttcc tgtgcttcat ctttaaactg caacacgtcg
    83221 atggtcgcca gcgctgagcc ggagatcatc agcatcagca cgcccaataa aaacctcatt
    83281 gtgcggcctc cttactgtat ttctcccaca gcggcttgat ctcttcttcc cagacgcgag
    83341 gattgagatc gcccgcatgg cgatagcgaa tgatgccgtt gccgtcaata agaaacgttt
    83401 ctggcgcgcc atagacaccg agatccagcc ctaacatgcc atcgccatca aaaaggctta
    83461 gcgcgtaagg attgcccagc tctttcagcc agctgattgc cttctggcga tcgtctttat
    83521 agttcatgcc gaccacgcgg atgccctgcg cagaaagctg attcagatat tgatgttccg
    83581 cacggcaggt cggacaccag gtcgcccaga cgttaagcag cactggtttg ccctgagtca
    83641 gcacatccgc ctgataaaac tgccccggat tgtccagtga ttcgagacga aacttcggca
    83701 caggcttgcc aatgagcgcc gattccagat tggtcggatc atccccttcg gcattacgcg
    83761 ccagctgcca cagcagcgcc gcggcaatcg ccaggaagat aatcaacgga attaacaata
    83821 ctttgcgctt catacagcct ccggcgcagt tttttgcggg ctcacgcgct tacgatagcg
    83881 aggatcaaac agacacagca gtccgcccaa cgccatcatc agcccgcccg cccaaatcca
    83941 gcgaacaaat ggtttgtagt aaagacgcac agcccacgcg ccgttttcca gctcttcacc
    84001 gagcgcggcg tacaggtcac gcgtgatgcc gccgtcaatt gccgcttcgg tcatcatcga
    84061 cccggcagtg ttgtaataac gtttttccgc atacagcacc gtttccggtt taccctctcg
    84121 cgttacgccg atagtcgcca caccgccacg ccagttcggg ccagtcacct ctttgacatc
    84181 acggaaggtg aagcgatatt catgaatatc gacgctatcg ccggacttca tgcgcacatc
    84241 acgctcaacg ctatagttct ggctaaaggc aatgccaaca attgtcactg ccagccccag
    84301 gtgagccgcc accatccccc aataactgaa ggtggttttc gtgccgcgtg aaatacgtag
    84361 cgcagcttcc gcaattgcca gcaccgcaat ccagcaggcc attgccaggc cgagcaccgt
    84421 catcgccaca actttgcttt cgaacagcca cggcaacagc agagacagca ccagcgtaga
    84481 gatgaaggcg ataatcaata aattgcggat tttacgtggg cgatcgcgcc cccagcgcac
    84541 cagaggaccg acacccagca gtagcgcaaa cggcaccatc agccaggtaa acatggtgtt
    84601 gaagaacggt tcgccaatcg aaatactgcc cagtcccagt tgcttgtgca ccagcggcag
    84661 cagcgtcccc agcaacacca ccagcatcgc ggcgaccagc aaaacgttgt tcgctaacag
    84721 caaagattcc cgcgaccaca gcgcattgtt tacgcgtgag cgaactttgt gtccacgcgc
    84781 ggcaaacagc agcagcgaac cgccaatcac cagcaccata aaggcgagga taaacatacc
    84841 gcgcgccgga tcagacgcaa acgcgtgtac cgataccagc acgccggaac gcacgaggaa
    84901 agtccccaac agacacaacg agaaggcact gattgccagc agtaatgtcc acgctttgaa
    84961 gctggcgcgt tgttcagtga ccgccagtga gtgcatcagc gcagtcccca ccagccacgg
    85021 cataaacgag gcgttttcta ccggatccca gaaccaccag ccaccccagc cgagttcgta
    85081 ataggcccat gcggaaccga gcacgatgcc gagcgtcagg aagatccacg ctgccagcgt
    85141 ccacggacga gtaaaacgcg cataagtgct gtccagacgc ccgctcagca aagaagcaat
    85201 ggcaaaagca aacgccaccg agaaacccac gtaccccata taaagcagag gcggatggaa
    85261 gatcagcccc ggatcctgta acagcgggtt aagatcgcgc ccttcaatcg ggaagttcgg
    85321 cagcgtgcgt gagaatgggt tagaggtaaa gagaatgaac agcaaaaagc cgacactgac
    85381 catccccatt atcgccagta cgcgggccac aatatccagc ggaatacgct gactaaaaat
    85441 cgccaccgca aaggtccagc cgctcatcag cagcacccac agcagcagtg agccttcatg
    85501 tgcgccccag gtagccgcca cgcgatacca caccggaagc tgggtattgg agttgctggc
    85561 aacataggtg acggtaaagt cgttgaccac gaaagcattg accagcacca gaaatgcgcc
    85621 agccacagac ataaacagca gccaggcaaa caagcgggaa gacgccatca tgcgcgcatc
    85681 tccgcgcgcc acgccccata gcggatacac ggacagcagc agcgcaattc ccagcgccag
    85741 gcacagcagc ccgttaccaa tttccggcat catgatgctg ggtccttata aacactcgcc
    85801 gggcgacggt ggttagcttc catcgctttc tcaacttctg gcggcgtgta gttttcatca
    85861 tgtttcgcca gcacttcttt tgcgaggata tgattgcctt tttccagctc gccctgcacc
    85921 acaacgccct gcccttcacg gaacagatcc ggcaaaatgc cttcgtaaga gacatccact
    85981 gagccttcag cgtcgtaaat ggtgaaggtc actttcagcg aattgggatc gcgctgcaca
    86041 ctacccggca tcaccatccc gccaacgcgc agacgctgac cgacttccgg catctgctga
    86101 gtttcacgct tgccgtagag aatttccccc ggcgtataaa agagatcgat attcgagcgc
    86161 agcgcatata gcaccagacc gatagtcagc gccagccctg ccaacacggc acaggcaatc
    86221 cacaagcggt ttttacggcg aatattcatg cagcctcctg ctgttgcgca gcacgtaaac
    86281 gcgcctcacg cgcccgctgt tgcgccacac cacgcagaat tgcgcgatgc tgcatcaccg
    86341 agtgcacgac caaaaccacc agcggaataa cggtcatcac caccgccagc cagacaaaaa
    86401 aggcgtaacc gcccattgcg aaaaattcat tccaggaagc aaatgcaggg gtcatttacg
    86461 gcctcttttc agtatcagtt cactcaccca cggacggcgt ttttccatca gcaaaatcaa
    86521 attacgcatc cgcatcagcg tcagcgtggc agagaggagc aggaagccaa aaatcgacca
    86581 gcgcagcggc gaacgcatcg ccggatcgat actttgctgc atccgcgttg atccctgatg
    86641 cagggtgttc caccactcca cggagtaatg aataatcggc agattcacca cgccaatcag
    86701 caccaggata cctgccgcac ggcccgccag acggcggtcg tcgaaggcgt gccacagggc
    86761 aatcacaccc acatacaaaa acagcagcac cagttcagaa gtcagacgtg catcccatac
    86821 ccaccaggtg ccccacatcg gttttcccca tgcagagccg gtaaccaggg caataaaggt
    86881 aaacacggca ccaatggggg ccatcgccgc caccgccagg ttggccattt tcatctgcca
    86941 gacaaggcca ataaacgccg ccaccgccat tgatgcataa atgcccatcg accagatcgc
    87001 cgcaggcaca tgcaggtaga taatgcggta gctatttccc tgctgataat cagccggagc
    87061 aaagccgaat ccccagatcc agccgacggt aagcacgact acactggcaa ttgccagcca
    87121 cggtataaac cagccacaga tttgatacag ccgtggtggg atcgccagtt gatgcagtgt
    87181 tttccacata gtttcgatac cagactcgaa caaaaatcag taatccagcg ttattgaatg
    87241 ctgattcgta acgctgccgc cgtcgcaaaa ggacttaatg tcgcggtgcc tgccagcaac
    87301 gcgcctaaaa ttgccagata cccgtcaacg ggcaaatgca tagaagccgc gtccatcgcg
    87361 gcggtggcaa agatgagtaa tgggatagtc agcggtaaca ccagtatgct gagcagcaca
    87421 ccaccgcgct taagtcccac tgtcagcgcc acgcccggtg caccgagaaa gccaagcgta
    87481 ggcgttccca gcagtagcgt taacgccatc acttgccagc cataaacatc cattcccagt
    87541 agcattgcta ccagtggcga aaggatgagt aacggcagac cggttaccat ccagtgcgcc
    87601 atcaccttcg ccagcacaac ggcgggcaag ggtaacggca acaacatcaa ttgttcaaga
    87661 ctgccgtcct gcaaatcgtc acggaacagt cgttccagcg ccagcaagga tgaaagcagc
    87721 gcggcaaccc agataatgcc cggtgcaata cgtgccagca gttgcggctc cggaccgata
    87781 ctgagcggaa aaagggtaat tacaatcagg aagaaccaca gcgggttggc gatttcagcg
    87841 ctatggcgaa acgctacgcg cagctcaaga cggaaaatgc gccagaacat catacggccc
    87901 tcgtttgcgt cagtgaaatg cggcgaattt tactttcagc aacgttgagc ggctggtggg
    87961 tagtgagaat cacaatcccc ccctgctctg tatgctgcgc catacgctgg gtcagacgat
    88021 cgacgccgtt aacgtcaatc gcggtaaaag gctcgtcgag gatccataac gtggcacggg
    88081 tcagccacag acgcgctaaa gcgacgcggc gttgttgccc ggcagaaagt tgatttaccg
    88141 gaatatcttc gaatccggca agcccggcct gcgccagggc ttccagacat tgtgcggtat
    88201 cgccatcgcg atgataaaag tgcagatttt ctaacgccgt cagccgggtt ttgatccccg
    88261 gctgatggcc tatccataac aggttttgat ggtagctgtc gcgtacctga tgcaagggct
    88321 gcccttgcca gagaacctcg cctgcgtcag ggcgagacaa ccccgtcagc aaacggagaa
    88381 gcgttgtctt ccccgcgccg ttgctaccgg tgatttgtac ccactctcct gcgttcagcg
    88441 taaatgacaa gccactaaat aaggttcgtt catcccgctc acaaagtaac tctctgactt
    88501 caagcatacc cacgcaataa ccctgttaaa aacctggctc gacttcacgc atatcgggca
    88561 gcttgtgcgc tatcccttta tggcaatcaa tacaggtttg cccatctttc accgcctggt
    88621 catgcatctt cgcggcaacc gatttctggg cggttgtatc catatactcg aagttgtgac
    88681 agttacggca ctcctgcgag ttattgtcct tcatgcgccg ccactcattc tgtgccatcg
    88741 tcagacgatg agcttcaaat ttctgcggcg tgtcaataac gccaaaaatt ttaccataca
    88801 gctctttact tgctttgagc ttgcgtatca tcttcggcac aaactcgtgc ggaacgtgac
    88861 aatccggaca ggtcgcacgg acgccgctac ggttgttgta gtgcacggaa tccatgtatt
    88921 cctgatacac cgtgttacgc atttcgtggc agctaatgca gaactcttcg gtattggctt
    88981 tttccatccc agtgttaaag ccgccccaga agacgatgcc gccgacaaaa ccgatcaaca
    89041 gcagcgtccc cagcgccaga cggctggggg tacgccacca tttccacagg cgcttaatca
    89101 gaccaggctt acggtcagaa tttcccataa taacctctta tttcccgtaa ccttttgatg
    89161 gggtaaaggt attccccacg attggcgcgg tatcggcctg cggtacgtga cattgcagac
    89221 agaaataacg acgcggagcc acttccgcgc ccactttgcc gtcgctgtcc ataaagtgag
    89281 taggactgat acgcggcgcg ccagtggtgc gatagctttc gacaccgtgg cactgcaagc
    89341 agcggttggt attggtcgtt acctgataac cttcaacgct atgcgggatc attggcggct
    89401 gattcacata gttcagcggc atccgatcct gctcttttgg catccgaatg gccccttcct
    89461 gcgtcccgga tacttccggc gactggctaa aatcgacgcc attagccgcc caaaccgcgc
    89521 cgcttaccac cagggccagc atcgccgtcc attgacacag cgctttcttc aggtcatggc
    89581 ttttcatgat ttcgctcccg aactccatcg tgtagttatt gtaaatacat cctcagaaca
    89641 gacatccacg cagcgaccgc acgtcatgca atcgcggctg gtgacctgca ccgggctttg
    89701 ttcatccagc accggggcac gtagcacatg cggttccggg caaacatgaa aacaatccat
    89761 acagcggtta catttctgcc gatcgctggc cgcaacggta atcacacctt tgctacccag
    89821 cacgccatac agcgcgccaa ccggacaaat gtgcccgcac cagccgtgtt caacgaccag
    89881 tagatcaaat aaaaacagcg cgagaataag cagcgcgccg ctgccgaagc ccattaccag
    89941 gctacgcccc atcagggaaa cggggttgat ccattcccag atgagcgtgc cagttaaggc
    90001 tgatcccacc agaatgacca ccaacagcac gtagcgaatg tggcgaggga tcgtcgcaga
    90061 ctgattgagg tcaaagcgcc tgcgtaacca gttcgctaag tcggtaatcg ggttcagcgg
    90121 gcagacccag ctgcaaaata atcgctttcc tgccagggcg tagagcacgg tgataatcac
    90181 cgcaccggtc aacgccaccg tggcaggcag atgaccactg gcaagacttt gcagcgtcat
    90241 cagcggatcg gtcagcggaa cggtgtcgaa taacaggcta ctgctgtagt tgccgtgcaa
    90301 gatccacaca ccaaaccacg gaccgctcaa aaacatcccc agcacgaaga actgacaaag
    90361 gcgacgtaac accagccaac ggtgactgcg ccaccagcct tttttctcca gcgcctcgcg
    90421 cccggcgtca cgtttacgat ttgccattgt tcccctccag ccagccgaag cggtaatggt
    90481 gacctaactc ccctttcgcc agtgacagcg gtaacacctt gattgccggt tgttccagca
    90541 cgcatacttt ttcgcactta ccgcaaccgg tacaggcgtc gctgtgaacc gtcggcagaa
    90601 agcgggcgtg cttaccggta cgcgtgttgc gctccagctc cagggtgatg gcctcatcaa
    90661 ttttcgggca ttcgcgataa caaacatcgc agcgcagccc ctgaaagttg agacagtttt
    90721 cctggtccac cagtaccacc agccccatcc gcgcgtcgtc gatcgattca atctcacgat
    90781 ccagcgcacc gctcgggcac actttggcgc acggaatgtc ctcacacatt tcgcaaggaa
    90841 tatcccgtgc gacaaaatat ggcgtgcccg ccgacaggcc agaggccagc gtcgccagtt
    90901 ttaaggtgtc gtaagggcaa gcctgaacac actgaccgca acgcacacag gcactggcaa
    90961 aggcgttctc gtttatggct ccgggcgggc gcaaccgcac gccagatgcg cgtgcggttt
    91021 gctgttgtaa ccccagcgcc acacccacgg cagccagccc gcctgctgtg cgaacgacat
    91081 cgcgcagaaa gcggcggcga ccattttgag gtttcgctga ccgggacata atggcttaca
    91141 ccttctccag tttgaccgcg cacttcttga agtccgtctc tttcgagagc ggatcggtcg
    91201 cgtccagcgt tagtttgtta accagctgtg cggcgtcgaa gaacggcatg tacaccaggc
    91261 cctgcggcgg acggttacga ccgcgcgttt cgacaatcga gatcacttcg ccacggcgag
    91321 aaaccacttt cactttgtcg ccacggcgca gatcgcgcgc tttcgcatcc agcgggtgaa
    91381 taaacaggac cgcttccggg aaggcacggt gcagttccgg cacacggcga gtcatactgc
    91441 cggtgtgcca gtgctccagg acgcgtccgg tagagagcca caggtcgtac tcttcatccg
    91501 gtgcttccgc cgccggttcg aacggcagcg cgaagatcac cgctttacca tccggtttac
    91561 cgtagaactt atagccttcg cccgctttca cgtacgggtc gttaccttcg ctgtaacgcc
    91621 actgcgtttc tttaccgttc accaccggcc agcgcagacc gcgcgctttg tggtagtcat
    91681 cgaacggcgc gagatcgtga ccgtgaccgc gaccaaacca tgcgtactct tcgaacagcc
    91741 ctttttgcag atagaagccc agctcgcggg attcatcgtt cagctgatct tccgccagtt
    91801 cggataccgg gaatttgctc acttcggggg tggcatacag aacttcgtac agcgttttgc
    91861 cacgcagttc cggtttcttc gccagcagct cttccggcca tacttcttca gttttgaagc
    91921 ggcgggagaa ctggactaac tgccagaggt ccgatttcgc ttcgcctggc gcctgtacct
    91981 gctgacgcca gaactgagta cggcgttcgg cgttaccgta agccccctct ttttctaccc
    92041 acattgcggt cggcaggatc aagtcggcgg ccagcgcact gactgtcgga tacggatcgg
    92101 agacgatgat gaagttgcgc ggatcgcgcc agcccggcat acgctcttca ttaatgttcg
    92161 gcccggcctg catgttgttg gtacacatgg tccagtaaac attcagcttg ccgtctttca
    92221 gcgcacggtc ttgtgctacc gcatgcagac cgattttcgc cggaatggtg ccgctcggga
    92281 tattccactt cttctcgcag atatcacgat gtttctcgtt agttaccacc atgtccgcag
    92341 gcagacggtg agcaaaggtg cccacttcac gcgcagtacc acacgcggaa ggctgcccgg
    92401 tcagggagaa cggaccgcaa cccggctggg aaattttgcc ggtcagcagg tgcaggttgt
    92461 agaccaggtt gttggcccac acgccacgag tatgctggtt gaagcccatc gtccagtagg
    92521 agatgacttt cttgttcgga tcggcataca gctgcgccag ttgttccagc tgatcttttg
    92581 gtacgccagt catttcagcg gttttttcca gcgtatactc agcaacgaag gctttgtaat
    92641 cttcaaagct catcggttcg gaggcgtcag aacccggatt cttcgctgct ttttccagcg
    92701 gatgggtcgg acgtaaaccg tagccgatgt ccgtcgcccc tttgcgcagg ttaacgtgct
    92761 tgctgaagaa gtcctgattt atcgcattgt tttgaatgat atagttggcg atgtagttca
    92821 ggatcaccag gtcagattgc ggcgtaaaga tgatgccgtt atccgccagc tcgaagctac
    92881 gatgctggta ggttgaaagc accgccacgg tgacgtcctg gttagagaga cgacggttag
    92941 tgatgcgtga ccagaggatc gggtgcatct ccgccatgtt tgcgccccac agcacaaacg
    93001 cgtcagcctg ctcgatgtcg tcatagcagc ccatcggctc atccatacca aaggttcgca
    93061 taaagccaac tactgccgac gccatacagt gacgcgcatt cgggtcgatg ttgttcgaac
    93121 ggaagcccgc tttaaacagc ttggacgcgg cataaccttc ccagatagtc cactgaccag
    93181 aaccgaacat accgatcgat tccggccctt tttctttcag ggcggttttg aatttctctt
    93241 ccatcacatc gaaggcctga tcccaggtga ttggggtaaa ttcgccttct ttgtcatatt
    93301 taccgttttt catacgcagc agcggctgcg tcaaacggtc tttaccgtac atgatcttgg
    93361 gcaggaaata gcccttaatg cagttcaggc cacggttaac cggtgcgtcc gggtcgccct
    93421 gacaggccac cacacgcccc tgctgcgttc cgaccagaac gccgcaacca gtaccgcaga
    93481 aacggcacgg cgctttatcc catttaatgg cttcctgctg accaacaacg gcgcgggcaa
    93541 cgcccggcac gctgagaccg gcagccgccg cagcggccgc aacggcgtta gctttcataa
    93601 agctacgacg actgagtttc atggtgtttc ctcaccttgc tcttcctgct ggtgataaac
    93661 cagcgacacc gccagcacgc cctctacgtt gcgtactgac tcaatggttt ggatcagcgt
    93721 ttcgctgtct tctgcttcca ccaccacaat caactgaccg ctcggcgcgt cgctgacagc
    93781 aacttcacag ccgggaaagg cgttcagttg ggtgctgatg tctgaaattc gttcgctttt
    93841 ggcctgcaca accaggctgc aaacttgcca gttagtgtgc atggagatac tccgcagtta
    93901 tggctgatac cggacagctg gctgcacatg ctccacatcc gttgcaaagt tggctattaa
    93961 gttgcggctg gtagatcccg gagagcgtcg ggcgaaagat aattgccatt ggctcacagc
    94021 tatcctgaca gcggcggcat tcaactgact gatacgccag gcacgcgtcc ccaatggtga
    94081 actgcaaatc ccaggccctg gtgtggcgcg gagaaaatag cgattcgggg caagcctgcg
    94141 cgcaggcgta acagaagctg cactcgttat ttttgaaatt aacgctcgga tagccgcccg
    94201 cgccgcgttg cagaatgttg ttttcacaag cattaataca ggcgtcgcag cgggtgcaat
    94261 gggtcagaaa atgagattca tcaccggacc agggcggacg gataccgtta ctggctttgc
    94321 gccagcgacc agtgagtatg ccccgacggg atgcatcaat cttcacattg accttccatc
    94381 attaacgcgc tctgaaaatt gagagcgacc aaataaaccg cataattaat aagccatttt
    94441 tatagccgct aagatattaa aggatgtgtc aaagatgcat accccgatcg aggtaaaacc
    94501 tgtagcagga tcaaaagagt ggcgggaagc gtggcaaaaa cgggcttttg ctcacatttc
    94561 aaatggttat aaatatattt atatagcgat taattcacca gagatatttc tgctggtttg
    94621 ctctctcatc agaatttaac actaaaagag caggtaaaat tgtctgaata ttctttaagt
    94681 tattcataaa gcaaattaat aaatctgatg aatatgttaa ccttcagcga catcatcggt
    94741 gcaaacctat aaatgaagaa ggaaagcaaa aaatgaagac cattctacct gcagtattgt
    94801 ttgccgcttt cgccaccact tccgcctggg cggcagagag cgtccagcca ctggaaaaaa
    94861 tcgcgcctta tccacaagct gaaaaaggca tgaagcgtca ggtgattcag ttaaccccgc
    94921 aagaagatga atctaccctg aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt
    94981 gcaatttgca tcgtctcggc gggaagctgg aaagcaaaac gctggaaggc tggggctatg
    95041 attattatgt ctttgataaa gttagctcgc cggtttccac gatgatggcc tgcccggatg
    95101 gcaagaaaga gaagaaattt gtcaccgcgt atctgggcga tactggaatg ctgcgttaca
    95161 acagcaagct gccgatcgtg gtgtatacgc cagacaatgt agatgtgaag taccgcgtct
    95221 ggaaggcgga agagaaaatt gacaacgcgg tagttcgcta aactgccgtg aagtgcggca
    95281 ccccgtaggt tggataaagc ggtcacgccg catccgacat ctaacgcccg agccggttgc
    95341 ctgatgcgac gctggcgcgt cttatcagca tacgccacat ccggcatacc atgccggatg
    95401 tggcacatca ttacaacgca atatccgcca cttctttttg aacgggttgc ggtttcaact
    95461 gcggtttcgg cgtactatct gctgcttgcg gtttgtcgta tttcagcccc agcacttcgc
    95521 tggtgtactg caactcgcgt tctgttgccg ctacatcacc gttcagcttg cgtccataag
    95581 atggaacgat cgctttcaac gtagcctgcc attgcgggct ggaaacgcga tcgccaaata
    95641 ctttttccag cagattcaac ataatcggcg cggcggttga cgcccctggc gatgccccca
    95701 ggagcgcggc aatggttcct tgctggtcac tgaccacttc agtacccaga cgcagtacac
    95761 cacctttatc ggcatcacgc ttgataatct gcacacgctg ccccgcttgc cacaaacgcc
    95821 agtcctcttt tttcgcttgc ggatagtact ctttcaacgc ttcaaaacga tcctcttcac
    95881 tcaacatcac ctgactcacc agatatttca ccagatcgaa attatccagc ccgacgtgca
    95941 tcatcggcat cacgttagag gtggtggtgg aactcatcag atcccacaat gaaccgtttt
    96001 tgaggaattt ggttgagaag gtggcaaatg gcccaaacag cactacgcgt ttaccgtcca
    96061 gaacgcgggt atcgatatgc ggaaccgaca tcggtggtgc gccaacggat gctttaccgt
    96121 aaaccttcgc cagatggtga ttaaccacgt ccgggttttc cgaaacaagg aactgtccgc
    96181 ccaccgggaa accggcgtag tctttcgctt ccggaatccc cgattcctgt aacagcttca
    96241 gtgccgcacc gcccgcgccg ataaagacaa atttcgcacg gatgttctgt gcagtgccat
    96301 ttttcagatc ggcaacggta acggtccagg tgttatcgtc attacgcttt agggcgcgga
    96361 cttcgctgct gagttgcagc gagaagttag atttcttctg caaggaagca attaactggc
    96421 gggtgatctc gccgtagttc acatcggtac caatttccgt acgcgtggct gccactttct
    96481 gttgcggatc gcgcccttcc atcactaacg gtgcccactc tttgatctgc gcgtgatctt
    96541 cagagtaacg cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca
    96601 ggaaattgac gttatcctcg ccccagacaa agctcatatg cggaacggta ttgataaatg
    96661 aacgcggagt acgcagcacg ccgcgctcga cctggtgcgc ccagaactgg cgggaaatct
    96721 gaaatgcttc gttaatggcg actgcttttt caatactgat gctgccatcg gcgttttgcg
    96781 gggtgtagtt cagttccatc agtgcagaat gcccggttcc ggcgttattc cagccgttcg
    96841 aactctcctg cgcgacaccc tccaggcgct ccaccatggt catcgaccat tcaggctcca
    96901 gctcgcgtaa ataggtcccc aacgtggcgc tcataatgcc gccgccaatc aacagtacat
    96961 cagtttcctg ctcctcggac gctttcgctt ttgccgccat cgaaacggca ttaagcccca
    97021 cggccatcga gaagagcatg gcagtcactt ttttcatctt gttaatgcct tacttttagt
    97081 cgctttattg caggtgagat ttgcgcggca tcaacggtaa cact
//
LOCUS       DAZGXS010000004        81891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGXS010000004 DAZGXS010000000
VERSION     DAZGXS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            31..198
                     /gene="yhfL"
                     /locus_tag="ACHKG5_000334"
     CDS             31..198
                     /gene="yhfL"
                     /locus_tag="ACHKG5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0866518.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            494..1831
                     /gene="frlA"
                     /locus_tag="ACHKG5_000335"
     CDS             494..1831
                     /gene="frlA"
                     /locus_tag="ACHKG5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP0866519.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1852..2874
                     /gene="frlB"
                     /locus_tag="ACHKG5_000336"
     CDS             1852..2874
                     /gene="frlB"
                     /locus_tag="ACHKG5_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP0866520.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2925..3752
                     /gene="frlC"
                     /locus_tag="ACHKG5_000337"
     CDS             2925..3752
                     /gene="frlC"
                     /locus_tag="ACHKG5_000337"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP0866521.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3752..4537
                     /gene="frlD"
                     /locus_tag="ACHKG5_000338"
                     /pseudo
     CDS             3752..4537
                     /gene="frlD"
                     /locus_tag="ACHKG5_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4637..5368
                     /gene="frlR"
                     /locus_tag="ACHKG5_000339"
                     /gene_synonym="yhfR"
     CDS             4637..5368
                     /gene="frlR"
                     /locus_tag="ACHKG5_000339"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP0866522.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5499..6503)
                     /gene="trpS"
                     /locus_tag="ACHKG5_000340"
     CDS             complement(5499..6503)
                     /gene="trpS"
                     /locus_tag="ACHKG5_000340"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0866523.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6496..7254)
                     /gene="gph"
                     /locus_tag="ACHKG5_000341"
     CDS             complement(6496..7254)
                     /gene="gph"
                     /locus_tag="ACHKG5_000341"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0866524.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7247..7924)
                     /gene="rpe"
                     /locus_tag="ACHKG5_000342"
     CDS             complement(7247..7924)
                     /gene="rpe"
                     /locus_tag="ACHKG5_000342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0866525.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7942..8778)
                     /gene="dam"
                     /locus_tag="ACHKG5_000343"
     CDS             complement(7942..8778)
                     /gene="dam"
                     /locus_tag="ACHKG5_000343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0866526.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8885..10171)
                     /gene="damX"
                     /locus_tag="ACHKG5_000344"
     CDS             complement(8885..10171)
                     /gene="damX"
                     /locus_tag="ACHKG5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0866527.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10263..11351)
                     /gene="aroB"
                     /locus_tag="ACHKG5_000345"
     CDS             complement(10263..11351)
                     /gene="aroB"
                     /locus_tag="ACHKG5_000345"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0866528.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11408..11929)
                     /gene="aroK"
                     /locus_tag="ACHKG5_000346"
     CDS             complement(11408..11929)
                     /gene="aroK"
                     /locus_tag="ACHKG5_000346"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0866529.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12330..13568)
                     /gene="hofQ"
                     /locus_tag="ACHKG5_000347"
     CDS             complement(12330..13568)
                     /gene="hofQ"
                     /locus_tag="ACHKG5_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0866530.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13480..13884)
                     /gene="hofP"
                     /locus_tag="ACHKG5_000348"
     CDS             complement(13480..13884)
                     /gene="hofP"
                     /locus_tag="ACHKG5_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0866531.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13874..14314)
                     /gene="hofO"
                     /locus_tag="ACHKG5_000349"
     CDS             complement(13874..14314)
                     /gene="hofO"
                     /locus_tag="ACHKG5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0866532.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14298..14837)
                     /gene="hofN"
                     /locus_tag="ACHKG5_000350"
     CDS             complement(14298..14837)
                     /gene="hofN"
                     /locus_tag="ACHKG5_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0866533.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14821..15615)
                     /gene="hofM"
                     /locus_tag="ACHKG5_000351"
     CDS             complement(14821..15615)
                     /gene="hofM"
                     /locus_tag="ACHKG5_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP0866534.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15735..18287
                     /gene="mrcA"
                     /locus_tag="ACHKG5_000352"
     CDS             15735..18287
                     /gene="mrcA"
                     /locus_tag="ACHKG5_000352"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0866535.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18452..19012)
                     /gene="nudE"
                     /locus_tag="ACHKG5_000353"
     CDS             complement(18452..19012)
                     /gene="nudE"
                     /locus_tag="ACHKG5_000353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0866536.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19332..21467
                     /gene="igaA"
                     /locus_tag="ACHKG5_000354"
     CDS             19332..21467
                     /gene="igaA"
                     /locus_tag="ACHKG5_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0866537.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21532..22200
                     /gene="yrfG"
                     /locus_tag="ACHKG5_000355"
     CDS             21532..22200
                     /gene="yrfG"
                     /locus_tag="ACHKG5_000355"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0866538.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22211..22612
                     /gene="hslR"
                     /locus_tag="ACHKG5_000356"
     CDS             22211..22612
                     /gene="hslR"
                     /locus_tag="ACHKG5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0866539.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22637..23515
                     /gene="hslO"
                     /locus_tag="ACHKG5_000357"
     CDS             22637..23515
                     /gene="hslO"
                     /locus_tag="ACHKG5_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0866540.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23578..25302)
                     /gene="yhgE"
                     /locus_tag="ACHKG5_000358"
     CDS             complement(23578..25302)
                     /gene="yhgE"
                     /locus_tag="ACHKG5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0866541.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25681..27303
                     /gene="pckA"
                     /locus_tag="ACHKG5_000359"
     CDS             25681..27303
                     /gene="pckA"
                     /locus_tag="ACHKG5_000359"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0866542.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27379..28731)
                     /gene="envZ"
                     /locus_tag="ACHKG5_000360"
     CDS             complement(27379..28731)
                     /gene="envZ"
                     /locus_tag="ACHKG5_000360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0866543.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28728..29447)
                     /gene="ompR"
                     /locus_tag="ACHKG5_000361"
     CDS             complement(28728..29447)
                     /gene="ompR"
                     /locus_tag="ACHKG5_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0866544.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29675..30151
                     /gene="greB"
                     /locus_tag="ACHKG5_000362"
     CDS             29675..30151
                     /gene="greB"
                     /locus_tag="ACHKG5_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0866545.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30248..32569
                     /gene="yhgF"
                     /locus_tag="ACHKG5_000363"
     CDS             30248..32569
                     /gene="yhgF"
                     /locus_tag="ACHKG5_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0866546.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            33007..33234
                     /gene="feoA"
                     /locus_tag="ACHKG5_000364"
     CDS             33007..33234
                     /gene="feoA"
                     /locus_tag="ACHKG5_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0866547.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33251..35572
                     /gene="feoB"
                     /locus_tag="ACHKG5_000365"
     CDS             33251..35572
                     /gene="feoB"
                     /locus_tag="ACHKG5_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0866548.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35572..35808
                     /gene="feoC"
                     /locus_tag="ACHKG5_000366"
     CDS             35572..35808
                     /gene="feoC"
                     /locus_tag="ACHKG5_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0866549.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            36011..36889
                     /gene="rpnA"
                     /locus_tag="ACHKG5_000367"
     CDS             36011..36889
                     /gene="rpnA"
                     /locus_tag="ACHKG5_000367"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0866550.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36916..37686)
                     /gene="bioH"
                     /locus_tag="ACHKG5_000368"
     CDS             complement(36916..37686)
                     /gene="bioH"
                     /locus_tag="ACHKG5_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0866551.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37724..38407
                     /gene="gntX"
                     /locus_tag="ACHKG5_000369"
     CDS             37724..38407
                     /gene="gntX"
                     /locus_tag="ACHKG5_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0866552.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38466..39041
                     /gene="nfuA"
                     /locus_tag="ACHKG5_000370"
     CDS             38466..39041
                     /gene="nfuA"
                     /locus_tag="ACHKG5_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0866553.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39402..40718
                     /gene="gntT"
                     /locus_tag="ACHKG5_000371"
     CDS             39402..40718
                     /gene="gntT"
                     /locus_tag="ACHKG5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0866554.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40763..42847)
                     /gene="malQ"
                     /locus_tag="ACHKG5_000372"
     CDS             complement(40763..42847)
                     /gene="malQ"
                     /locus_tag="ACHKG5_000372"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0866555.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42857..45250)
                     /gene="malP"
                     /locus_tag="ACHKG5_000373"
     CDS             complement(42857..45250)
                     /gene="malP"
                     /locus_tag="ACHKG5_000373"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0866556.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45862..48567
                     /gene="malT"
                     /locus_tag="ACHKG5_000374"
     CDS             45862..48567
                     /gene="malT"
                     /locus_tag="ACHKG5_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0866557.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48634..48912
                     /locus_tag="ACHKG5_000375"
     CDS             48634..48912
                     /locus_tag="ACHKG5_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0866558.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48903..49388
                     /locus_tag="ACHKG5_000376"
     CDS             48903..49388
                     /locus_tag="ACHKG5_000376"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0866559.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49438..50466)
                     /gene="rtcA"
                     /locus_tag="ACHKG5_000377"
     CDS             complement(49438..50466)
                     /gene="rtcA"
                     /locus_tag="ACHKG5_000377"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP0866560.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50470..51696)
                     /gene="rtcB"
                     /locus_tag="ACHKG5_000378"
     CDS             complement(50470..51696)
                     /gene="rtcB"
                     /locus_tag="ACHKG5_000378"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0866561.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51884..53482
                     /gene="rtcR"
                     /locus_tag="ACHKG5_000379"
     CDS             51884..53482
                     /gene="rtcR"
                     /locus_tag="ACHKG5_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP0866562.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53464..54222)
                     /locus_tag="ACHKG5_000380"
     CDS             complement(53464..54222)
                     /locus_tag="ACHKG5_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0866563.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(54239..55069)
                     /gene="glpG"
                     /locus_tag="ACHKG5_000381"
     CDS             complement(54239..55069)
                     /gene="glpG"
                     /locus_tag="ACHKG5_000381"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0866564.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(55114..55440)
                     /gene="glpE"
                     /locus_tag="ACHKG5_000382"
     CDS             complement(55114..55440)
                     /gene="glpE"
                     /locus_tag="ACHKG5_000382"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0866565.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55630..57135
                     /gene="glpD"
                     /locus_tag="ACHKG5_000383"
     CDS             55630..57135
                     /gene="glpD"
                     /locus_tag="ACHKG5_000383"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0866566.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(57350..57952)
                     /locus_tag="ACHKG5_000384"
     CDS             complement(57350..57952)
                     /locus_tag="ACHKG5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866567.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57952..58944)
                     /locus_tag="ACHKG5_000385"
     CDS             complement(57952..58944)
                     /locus_tag="ACHKG5_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0866568.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58970..60478)
                     /locus_tag="ACHKG5_000386"
     CDS             complement(58970..60478)
                     /locus_tag="ACHKG5_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0866569.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60603..63050)
                     /gene="glgP"
                     /locus_tag="ACHKG5_000387"
     CDS             complement(60603..63050)
                     /gene="glgP"
                     /locus_tag="ACHKG5_000387"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0866570.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(63069..64502)
                     /gene="glgA"
                     /locus_tag="ACHKG5_000388"
     CDS             complement(63069..64502)
                     /gene="glgA"
                     /locus_tag="ACHKG5_000388"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0866571.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64502..65797)
                     /gene="glgC"
                     /locus_tag="ACHKG5_000389"
     CDS             complement(64502..65797)
                     /gene="glgC"
                     /locus_tag="ACHKG5_000389"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0866572.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65815..67788)
                     /gene="glgX"
                     /locus_tag="ACHKG5_000390"
     CDS             complement(65815..67788)
                     /gene="glgX"
                     /locus_tag="ACHKG5_000390"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0866573.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67785..69971)
                     /gene="glgB"
                     /locus_tag="ACHKG5_000391"
     CDS             complement(67785..69971)
                     /gene="glgB"
                     /locus_tag="ACHKG5_000391"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0866574.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(70244..71347)
                     /gene="asd"
                     /locus_tag="ACHKG5_000392"
     CDS             complement(70244..71347)
                     /gene="asd"
                     /locus_tag="ACHKG5_000392"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0866575.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71539..72132
                     /gene="yhgN"
                     /locus_tag="ACHKG5_000393"
     CDS             71539..72132
                     /gene="yhgN"
                     /locus_tag="ACHKG5_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0866576.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(72178..73473)
                     /locus_tag="ACHKG5_000394"
     CDS             complement(72178..73473)
                     /locus_tag="ACHKG5_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP0866577.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(73380..75504)
                     /locus_tag="ACHKG5_000395"
                     /pseudo
     CDS             complement(73380..75504)
                     /locus_tag="ACHKG5_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75644..76984)
                     /gene="gntU"
                     /locus_tag="ACHKG5_000396"
     CDS             complement(75644..76984)
                     /gene="gntU"
                     /locus_tag="ACHKG5_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0866578.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76988..77515)
                     /gene="gntK"
                     /locus_tag="ACHKG5_000397"
     CDS             complement(76988..77515)
                     /gene="gntK"
                     /locus_tag="ACHKG5_000397"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0866579.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77654..78649)
                     /gene="gntR"
                     /locus_tag="ACHKG5_000398"
     CDS             complement(77654..78649)
                     /gene="gntR"
                     /locus_tag="ACHKG5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0866580.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78873..79568)
                     /gene="yhhW"
                     /locus_tag="ACHKG5_000399"
     CDS             complement(78873..79568)
                     /gene="yhhW"
                     /locus_tag="ACHKG5_000399"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0866581.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79691..80728)
                     /gene="yhhX"
                     /locus_tag="ACHKG5_000400"
     CDS             complement(79691..80728)
                     /gene="yhhX"
                     /locus_tag="ACHKG5_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0866582.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81062..81550
                     /gene="yhhY"
                     /locus_tag="ACHKG5_000401"
     CDS             81062..81550
                     /gene="yhhY"
                     /locus_tag="ACHKG5_000401"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0866583.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            81761..>81891
                     /locus_tag="ACHKG5_000402"
     CDS             81761..>81891
                     /locus_tag="ACHKG5_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866584.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQ"
BASE COUNT        19782 a        22221 c        20509 g        19379 t
ORIGIN      
        1 taatcaatat ataatacata gggaatgtaa atgaacaaat ttattaaagt tgcactggta
       61 ggtgcagtac tggctacgtt aactgcatgt actggtcata ttgaaaaccg tgataagaac
      121 tgctcttacg actacctgct gcacccagca atttctattt ctaaaatcat tggcggttgc
      181 ggtcctactg cacagtaagt ctctgacaga aatcagctaa cgctgattta attgcctgac
      241 gccctcggga ttacccgagg gcgtatttgt ttctatatcc ttcttgctcc ccacccgtaa
      301 aaatattttc actttaattt caattggata accacattca tcagccacag cgcgatcctg
      361 tgcgaaatta tgtgatcttc ctccacatta cataacatca tatgttgtta tattcatcat
      421 gcattgtcat gtcacctttt aaatgactgc aaactctccc ctacaacgtg ttggacaaga
      481 aaaaggtatc gctatgggaa gccaggaact ccaacgcaag ctcggatttt gggccgttct
      541 tgcaatcgcc gtcgggacaa ccgtcggctc cggtattttt gtatctgtgg gtgaagtggc
      601 aaaagcggcg ggcacgccgt ggctgacggt gctcgcattt gtcatcggcg ggttaattgt
      661 gatcccgcaa atgtgcgtat atgcggaact atccaccgct tatccggaaa atggcgcaga
      721 ttatgtttat ctgaaaaatg ccggaagccg accgctggct ttcctctccg gctgggccag
      781 cttctgggcc aacgatgcgc cgtcattgtc gattatggcg ctggcgattg tcagcaatct
      841 tggcttttta acgcctatcg atccgttgct cggtaaattt atcgccgccg gattaattat
      901 cgcctttatg ctgctgcacc tgcgctccgt tgaaggcggc gcagcgtttc agacgctaat
      961 taccatcgcc aaaattatcc cgttcactat cgtcattggt cttgggatct tctggtttaa
     1021 agcggagaat tttgccgccc ctgctaccac tgcgattggc gcaacgggca gctttatggc
     1081 gctgctggcg gggatctctg ccaccagttg gtcgtatacc ggcatggcct ctatctgtta
     1141 tatgaccggc gaaattaaaa accccggcaa aaccatgcca cgagcgctga ttggttcctg
     1201 tctgctggtt ctggtgctct acaccctgct ggcgctggtg atttccggcc tgatgccctt
     1261 cgacaaactc gccaattctg aaacgccgat ttccgacgcc ctgacctgga tccccgcact
     1321 cggcagcacc gctgggatct ttgttgccat cacggcgatg atcgtcattc ttggttcgct
     1381 ttccagctgc gtgatgtacc agccgcggct ggaatacgcg atggcgaaag acaacctgtt
     1441 cttcaaatgc ttcggccatg tgcatccgaa atacaacacg ccggatgtct ccatcatttt
     1501 gcaaggggcg ctggggatct tcttcatctt cgtttccgat ctcaccagcc tgctgggcta
     1561 tttcaccctg gtaatgtgtt tcaaaaatac cctcaccttc ggctccatca tctggtgtcg
     1621 taaacgcgac gattacaaac cgctgtggcg tactccggct ttcgggctga tgacccccct
     1681 cgccattgcg tcaagcctca ttctggtcgc ctcaaccttt gtctgggcac cgattcccgg
     1741 ccttatctgc gccgtcatcg ttattgccac tggtctgcct gcttacgcct tctgggcgaa
     1801 gcgtagccgc cagctcaacg ctttgtcgta atgttatctg gagaaaataa aatgttggat
     1861 attgataaaa gcactgtgga ctttctggtc accgaaaata tggttcagga agtggaaaaa
     1921 gttctcagcc atgacgttcc gctggtgcac accatcgtgg aagagatggt gaagcgcgac
     1981 atcgatcgta tttatttcgt tgcctgcgga tcgccactca acgcggcgca aacggcgaaa
     2041 catctggcag atcgcttttc cgatcttcag gtttacgcca tttccggctg ggagttctgc
     2101 gataacaccc catatcgcct cgacgctcgt tgcgcggtga ttggcgtttc tgactacggt
     2161 aaaaccgaag aggtaatcaa agcgctggag ctgggccggg cctgcggcgc actcactgcg
     2221 gcgttcacca aacgcgcgga tagcccgatt acctcggcgg cggaatttag cattgattat
     2281 caggccgact gtatctggga aattcatctg ctgctctgct acagcgtggt gctggagatg
     2341 atcacccgcc tcgcgccgca cgcggaaatc ggcaagatca aaaacgatct caaacagctg
     2401 ccgaatgcgc tcggttatct ggtacgcact tgggaagaaa aaggccgcca gcttggtgaa
     2461 ctggccagcc agtggccgat gatttacacg gttgctgcag gcccgctgcg cccgctgggt
     2521 tacaaagaag gcatcgtgac gctgatggaa tttacctgga cgcacggctg tgtgattgag
     2581 agcggtgagt tccgtcacgg cccgctggag attgtcgaac cgggcgttcc gttcctgttc
     2641 ctgctcggca atgatgaaag tcgccacacc accgaacgcg ccattaactt tgttaaacag
     2701 cgtactgaca acgtgatcgt catcgattac gccgcaattt cgcaaggact gcacccgtgg
     2761 ctggcaccgt tcctgatgtt cgtgccaatg gagtggctct gctactacct gtctatttac
     2821 aaagatcaca acccggatga acgccgctat tacggtggtc tggtggaata ttaatcctct
     2881 ctcccggccc ggcaacgggc cggatttacg caaggagtta cccgatgaaa acaggtatgt
     2941 ttacctgcgg ccaccagcgg ctgcctattg aacatgcatt ccgtgatgca agcgagctgg
     3001 gttacgatgg catcgaaatt tggggcggcc gcccgcacgc gttcgcgccg gacttaaaag
     3061 cgggcggcat caaacaaatc aaggcgctgg cgcagacgta tcagatgccg attatcggtt
     3121 acacgccaga aaccaacggc tatccgtata acatgatgct gggcgatgaa catatgcgtc
     3181 gtgaaagcct ggacatgatc aagctggcga tggatatggc aaaagagatg aacgcgggtt
     3241 atacgctgat ttctgcggcc cacgcgggct atctcacgcc gcctaacgtt atctgggggc
     3301 ggctggcaga gaacttaagc gaactgtgtg agtacgcgga aaacatcggc atggatctga
     3361 tcctcgaacc gttaacgccg tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc
     3421 atgcgctggc gctggtgcct tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt
     3481 atgtccaggc ggaaccggtg atgagttatt tcgacaaact gggcgataaa ttacgtcatc
     3541 tgcatattgt cgacagcgac ggggccagcg acacgcatta cattcccggc gaaggcaaaa
     3601 tgccgctgcg ggaactgatg cgcgatatta ttgatcgggg ctatgaggga tactgcacag
     3661 tggaactggt aacgatgtat atgaacgagc ccagacttta cgcccgccag gcgctggaac
     3721 gctttcgcgc gctgctgccg gaggatgagt gatgaaaacc ctggcgacaa tcggcgataa
     3781 ctgcgtcgat atctacccgc aactgaataa agcgttttct ggcggtaatg cggtgaacgt
     3841 ggcggtgtac tgcactcgct acggtataaa gccgggatgc attacctggg ttggtgacga
     3901 tgactacggc acaaagctta agcaggatct cgccagcatg ggcgtcgata tcagccatgt
     3961 ccatacgaaa cacggcgtta ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt
     4021 ttttggcgac tacaccgaag gcgtgatggc cgactttgcc ctgagtgaag aggattacgc
     4081 ctagctggcg cagtatgaca ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt
     4141 cccacagctg cacgctgcgg gcaaacttac cgctttcgac gtctccgaca agtgggacag
     4201 cccgctctgg cagacactgg tgccgcatct cgattttgcc tttgcctccg caccgcaaga
     4261 agacgaagcg ctgcgtctga agatgaaagc gattgttgcc cgtggcgcag gcgtggtgat
     4321 tgtcacgttg ggtgaaaacg gcagtattgc ctgggatggc gtgcagttct ggcgtcaggc
     4381 tcctgaacag gtgacggtta tcgacaccat gggtgccgga gattcgttca ttgccggatt
     4441 cctttgcggc tggtctgcgg ggatgacatt accgcaggcg atggcgcagg gaacggcgtg
     4501 cgcggcacaa actattcagt accacggtgc ctggtaggca taacgttggc gtgagcatct
     4561 tcacgccaac gtgctgttac ttgccggaaa acgaccctat aatccgtgta attcattctt
     4621 tatttcaggg tcgattatgt cagctacgga ccgctactct catcaactcc tctacgctac
     4681 cgtccgccag cggctgctgg atgatatcgc gcagggggtt taccaggccg ggcaacagat
     4741 ccctaccgaa aatgagcttt gtacacaata taacgtcagc cgcattacca ttcgcaaagc
     4801 catcagcgac ttagtggcag acggcgtact gatccgctgg cagggcaaag gcacctttgt
     4861 acaaagccag aaggtcgaaa acgccctgct gaccgtcagt ggttttaccg attttggcgt
     4921 ctcacaaggc aaggcgacga aagagaaagt gatcgagcaa gaacggatca gcgccgcgcc
     4981 gttttgcgaa aagctcaata tccccggtaa cagcgaagtg ttccatcttt gccgggtgat
     5041 gtatctcgat aaagagccgc tatttattga tagttcgtgg atcccgctgt cgcgttatcc
     5101 tgactttgat gagatttacg tcgaaggaag ctccacctat cagttatttc aggagcgttt
     5161 tgacacgcga gtggtcagcg acaaaaagac catcgatatc tttgccgcca cccgcccgca
     5221 ggcaaaatgg ctgaaatgcg aactgggcga accgttgttt cgcatcagca aaatcgcctt
     5281 tgatcagaat gacaaaccgg tgcacgtctc cgaactcttc tgccgcgcca accgcatcac
     5341 cttaacaatt gataataaaa gacattaacc gtaggccgga taagatgcac cagcatcgca
     5401 tccggcgatg ccggcgcact gaattttaca tcccgcacgt tcccctcacc ctaaccctct
     5461 ccccaaaggg gcgaggggac cgtccgagcg ttttgatatt acggcttcgc cacaaaacca
     5521 atcgcttcgt acaccgcttt tagcgtacgg gaagcgtgcg cgctggcttt ttctgcgccg
     5581 tctttcatca cctgttgcag gaaggcttca tcgttgcgga aacggtggta acgttcctgc
     5641 aattcagtca gcataccgga aacggcatca gccacttcgc ctttcagatg accatacatc
     5701 ttgccttcga actgtttttc cagttccggg atgctctggc ccgttaccgc agaaaggata
     5761 tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt agcgaactac cggcggctcg
     5821 tcggagtcgg tgaccgcgcg tttgattttc ttcactaccg atttcggatc ttccagcagg
     5881 ccaataacgt tattgcggtt atcgtcagac ttggacatct tcttggtcgg ctccagcagc
     5941 gacattacgc gcgcgccgga tttcggaata aacggctccg gcaccttaaa gatatcgccg
     6001 tacagcgcgt tgaaacgctg ggcgatatca cggctcagtt ccaggtgctg tttctggtct
     6061 tcacccaccg gtaccagatt agtttgatac agcaggatgt ccgctgccat cagcaccgga
     6121 tagtcaaaca gaccagcgtt gatgttctcg gcataacgcg cagatttatc tttaaactgc
     6181 gtcatgcggc tcagttcgcc gaagtaggta tagcagttca gtgcccagcc taactgcgca
     6241 tgttccggta cgtgggactg aacaaaaatg gtgcttttct caggatcgat accacaagcc
     6301 agatacaagg ccagcgtatc cagcgtcgct ttacgcagct tctgtgcatc ctggcgcacg
     6361 gtgatcgcgt gttggtcaac gatacagtaa atgcaatggt agtcatcctg catgtttacc
     6421 cactgacgca gcgcacccat gtagttacca atagtcaatt cacctgaggg ctgtgcgcca
     6481 ctaaaaacga tgggcttagt catttttcga ttcctgattt tcgctatgcg gaagccctaa
     6541 tgcgggcaga aggtcattta tagactgata aattacatca ggctggctga gatcgatagc
     6601 ctcgccgtag ttatatccgt aggttaagcc aactgatggg caacctgccg cttttgctgc
     6661 ctgaatatca ttgcgtgagt cgccaacaaa cagcatctgt tgtggagcaa ttcccatccg
     6721 ctcagccacc agtaacagcg ggtccggatg cggttttttg ttttgcacat catcaccacc
     6781 aatcaccacg ctgaagtatt tggcgatatc taaggcttcg agcagcggcg cgacgaacgg
     6841 cgtcggtttg ttggtgacca ggcctagcgg caggccttta gcctgcaacg cgcccaacgt
     6901 atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca acctcgccat agtaacgatc
     6961 gaacagttta cgcagaatac gtacctgttc ttctgccgga atgtcgtcat caacgggcgg
     7021 tttacccatt gttttacgca gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc
     7081 catcagaaca tctgcgccgt taccaatcca ggtaataacg cgttcttcac ctgcgacggg
     7141 caactccagc gcatacagcg ccatatctac cgcagcagca agaccgggag cactgtccac
     7201 cagcgtaccg tcgagatcaa aagcgacgcc gcgaatatct tcaaacttac tcatgactta
     7261 cctttgccag ttcactgcgc atttcatcaa tgactttttt gtagtctggc tggtcgaaga
     7321 ttgccgaacc ggcaacgaac atatccgcgc ccgccgcggc gatttcgcca atgttgttca
     7381 ccttcacgcc accgtccact tccagacgaa tgtcaaagcc agactcgtcg atacgacgac
     7441 gtacttcgcg cagtttatcc agtgtttgag gaatgaaaga ttgaccgccg aaaccagggt
     7501 tgacggacat cagcaggatc acatccagct tatccatcac gtaatccaga tagctcagag
     7561 gtgtcgccgg gttaaatacc agacccgctt tacagccatt ttctttaatc agttgcagcg
     7621 tgcggtcaac atgctcggag gcttctggat gaaaggtaat gatgctggca ccagcggcag
     7681 cgaaatcagg cacaatgcga tcgaccggtt tcaccatcag gtgtacgtcg ataggggcgg
     7741 taatgccgta gttacgcaag gatttcagca ccattggccc aatcgtcaga ttgggaacgt
     7801 agtggttatc catgacgtca aaatgcacga catcagcgcc agctgccagg gcttttgcgg
     7861 tatcttcacc caggcgggca aaatcagccg acagaattga gggggcaatc aaatactgtt
     7921 tcatccgctt ctccttgaga attatttttt cgcgggtgaa acgactcctg gtttgtacaa
     7981 agccagcagt tcgtccacct ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac
     8041 tttgacgaca tgcaattttg cgcgctgata ccactcacgc gttaacatcg tatcgtgatt
     8101 ggagatcagc acaggaatat ggcgctcaac cagaccttcg gcgatctccg ccagatgcgc
     8161 ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg tcgcagacag
     8221 cggtgcataa ggtggatcgc aatagacgac agatgcatca tctgcgcgcg ccatgctatc
     8281 ggcgtaagac tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc
     8341 tgcttccggg aaatagggtt ttttgtagcg gccaaacggc acattaaact caccgcgcag
     8401 attgtaacga cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg
     8461 acggaacgga tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa cctcggcgca
     8521 atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag tacgcatctt
     8581 cacaatgtta tagagactga tcaggtcgct attgatatcg gcaaggatat aacgagaaaa
     8641 gtcggtgttg agaaacaccg acccggcacc tacaaaaggc tcaaccagac attcgccctt
     8701 gggcaaatgc cgtttaatat catcaagcag gggatacttg ccccctgccc acttcaaaaa
     8761 agcgcgattt ttcttcatgc tgactaacta attacacctt ctccggctgt ggagaaagct
     8821 ccgacagcat ccagcgcttc aggcaactcc cgcaacgata aaccattgcg ggagataacc
     8881 ttgattactt cagatcggcc tgtacctgac gcagcggttt cgcccacggg tttttggcct
     8941 gaacatctgc tggcaatgta gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc
     9001 cagaaaccag gacataccac ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt
     9061 tcagattctc tttcttcgcc caaccgttca ggttgtcgta gttagaggaa ctgctcagct
     9121 gcagagtgta atggctggac ggtgccgatt tcaacgaacc cacattacct gcggtcttcc
     9181 ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact ggagccgtag
     9241 tcgccgtctc ttttggtgct ggcgtagaag tcgcagccgg tgccttcgtg ctcgccacag
     9301 gagcagctgg ttcagtacgc ttcggcgtct gtgctaccgg cttcggttcc gttttcacgg
     9361 ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg agctggacgc gtagtggccg
     9421 gacgttcagc agtttgcgtt ttcgccgtgt cacgcgatgc attgccattg cgtactggcg
     9481 cgacagttgc tggttcagtc ggcaatgtag aattgaccgc cacattgttc aactgttgct
     9541 gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca cgttgttgac
     9601 catccgttgc cgccggggtt tgcccttgag tcggcgtaga agagatcggt ggcagagaaa
     9661 catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg ccattcgcct
     9721 gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt tgatcgctgg
     9781 aagtggtcga gggggctttt agcgcagaac cgataccgat gatcaacagc agtagaacca
     9841 gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct gcttttttgc
     9901 gcttacgcgg acgacgctct acgcgctctt catccacggt ttcatcttcg gattcgtcaa
     9961 tttcttcttc gatttccggt tcctcattgc gctctttttg cgcacgagtc ggacggcgat
    10021 cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca cgttcagtac
    10081 gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg ggatcgggtt
    10141 tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg ttaatgctta
    10201 ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga cctttcttgt
    10261 tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga aacgccgctg
    10321 cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc cgcaaggact
    10381 ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg cggcccattg
    10441 accggtaacc cagcccgcgt gagcagagtt ataatacgct gcgtttcggc agaactaaac
    10501 tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac cgcttcacca
    10561 tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt gtgtcccaga
    10621 ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc tgccttcagt
    10681 tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa caacgcatcc
    10741 agattctctt ccagccagtt aaaaaacgta ccgtcaagaa taatgccgta tttgatgact
    10801 tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc gagatccacc
    10861 accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg atggttgacc
    10921 gcagttttgc cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg gacttgaatg
    10981 aaacgaacac cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc cactacgccg
    11041 ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa cgccgtaaag
    11101 acggtatcga gtacagccag gcttttatac tgctcgccgt caggaaggat aacgctatcg
    11161 acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag aggagccagg
    11221 gtttcgttgg tgaccaacat gacctgctcg cccgatttca gcggtaagaa tgaagctggt
    11281 tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag agtaacgaca
    11341 atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag tgtatataaa
    11401 gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcaaccact ttagcgcttt
    11461 gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg cgttcattgg
    11521 ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg cgttttttat
    11581 cacgctgcgt gcgtgcaagt tgcttttcga tcgtcgtttc aagataaacg acaacgccac
    11641 gagcggaaag acggttacgc gtttcacggg atttcacaga gccgccgcca gtagccagca
    11701 caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg cggaagcctt
    11761 cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc tcaatctctt
    11821 gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca atagtgcttt
    11881 ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt ttttcggtac
    11941 tactaagact attcgttaat gataaacccg cttcgctcag agagctccgc aggacatgaa
    12001 ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata ctccagcggg
    12061 tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa gcgcgctgcc
    12121 ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa agacagcaaa
    12181 atgccgcctg aatgataaat catcatcatg gaaggcaggc agcgaagagc accaactctc
    12241 aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa acacgttcag
    12301 cgtaaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg tggcgtgata
    12361 aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa ttgcccgaac
    12421 cagggaatgt cgccaagcaa cggcacgcta tcctgacccg atttattttt acgggtaaaa
    12481 atgccgccca gcgccaacgt ttctccgctt ttgacctcga cctgcgtttc gatctcctgc
    12541 ttatcaatcg ccagcacttc gccatcggcc tgctgtagca cctgccccgg aacgttctgg
    12601 ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt gacctccatc
    12661 cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct ggaaacctga
    12721 tatggaattt cgctcccctg tttaatgctg gcaggctgaa gatgtgaggc cagcagacgc
    12781 ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc aagatccagc
    12841 aaacgtccgt tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt cgctacggag
    12901 aggtcgctac caagcgtggt gacttgccca acgccaccag cgtgttgcgc atcggccagc
    12961 gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat atgcgccgac
    13021 agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag ggcgcttaac
    13081 gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt catactccct
    13141 ttggcactca gtagcttctc cccagctttc gccagctctc cggcgtcggc gtattgcaga
    13201 gtaatattgc gattttccag cggcagattt gcctgcgccc gcgcctgctc cgcttcctga
    13261 cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt gccctcctgg
    13321 cgcgttatca gtccggcgct tttcactaca gtttgtagtg cctgcttcca gggaacatct
    13381 gttagatgta acgacaccgt accgctgacg tctggcgaca ccaccaggtt caacttctcc
    13441 tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat cagcgtcact
    13501 ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc ggctatccat
    13561 tgcttcattt gtatctcctt gccgttgcca caaccattgt ggcggttcgc agtttgtccc
    13621 ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt gtccagccgt tttccagcac
    13681 atcgttttgc tgcacccgtc gccatttctt ttgcccgtct tttattacac cgatgatgcg
    13741 ctcgcctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa tccggcatag
    13801 atcttccggc ggtttaaaag ggtcacgcat accggttaaa aggcacaatg caatacctgc
    13861 caacaaccag cgtttaaccc tcatgcggcg tctccagttg tagggtgaac aaaagatcat
    13921 caccttccac gcttaatgaa aaacggctca cgctgacgtt gcgctctgcc agccgtgtaa
    13981 atgccgacgg cactgcttcc cacagcgttt tcaacgccaa ctcgcctccc tgcgcggatg
    14041 gatgccagaa aaccagttgt gcgccgggca actgaaaatc cagtggcgaa aagggcagcg
    14101 ttttttcttc gctgaaggga gtggtgtcaa ccaggcgata cagtgtggcc caatgctgat
    14161 gatgacttgc ccgtagtcga attaatgcgt cgcgctcttc atggtgtgtc gatgacagaa
    14221 aaatgagcgt aactaacatc agcaaccaga atgcccagca aaactggcgg aggcggggtg
    14281 atgtggcgaa ccaccagtca aagaacatgt tcatcgctaa ccttccttgt taactgatac
    14341 tcaaattgcc agcgtccctg cgcatcctgc tgcgtggccc cccgctgatt gagatgaaaa
    14401 gatgcatcct ggcggagtga cgtttctagt gcgtttaacg cggtaatgct tgttgtaagc
    14461 cccttgatct ccagcgttcc ctgctgccag cttatcgttg tcagccaggc gtgctcgggt
    14521 aaaagcgccg ccagtgcttc cagcgcagat tgccagtcgc gggtaaattg tcgctggcgc
    14581 tgcctttgca aacgctgctc acgaatttgt tggcgctcca gcaaacgtgg tttcgttatc
    14641 tgtaagctgc gggcgagttg ttgttccccc tgaagcaaaa cggcgtctac gcgagcttcg
    14701 gcgctgcttg tcagacgcag tattagcgtt atcccgacgg ccagcagcag aggcgcaacg
    14761 aacatcagca accagaaacg aagaaaagcg gtccggcgtt gctgtcgcca gggcaaaaaa
    14821 ttaattggcg ggttcatcag tacgccttcc caagcgccag cccagcgcga tggcaaagtc
    14881 tccacctggc ggcggtaacg gcggctgacg aacagaaacg gcctcccaag gatcaaatcc
    14941 gccttcgcca catatcgcga cgctttcggg atcaacggat aacgctgccg ccagctcctt
    15001 cgcgctagtc atccccaccg ccagtttgcg cccccagcga tagcgcgtcg cccacagcca
    15061 ctgttcgtta tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg
    15121 ctgtaatgca ctggcatccg gggtgatcgc actaacatga acacgcaacc tttctgccaa
    15181 agtaagcagc gttgccagct ctttgctttg cgcggcagtc acgttatagg cgggactgag
    15241 tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgagccat
    15301 cgtcccggac agccaggccg tttgctcccg ctcaccgagg gacatcgacg ggcgcggaaa
    15361 tgaccgctgt aatgtgcgac tggcgggaaa cgccaacatg atgtgatggc gctgcggcag
    15421 ttcgcgactc cacggtaaca acgttttagc cagccgctgc gcatcaacaa tccgcccatc
    15481 tttgataatg tcgtgcgcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgc
    15541 gccccgcaca atagcaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca
    15601 gatcttaaat gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat
    15661 gcgtctggct tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa
    15721 atgggaaatt tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc
    15781 tgctgggagc aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg
    15841 tggcgacatt aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg
    15901 agctgattgc tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccgc
    15961 cggagatggt gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg
    16021 ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac
    16081 aaggggcaag taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca
    16141 cgctgatgcg taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga
    16201 aagacgagat cctcgagctt tatctgaaca agatttacct tggttaccgc gcctatggtg
    16261 tcggtgctgc ggcacaagtc tatttcggaa aaacggtcga ccaactgacg ctgaacgaaa
    16321 tggcggtgat agccgggctg ccgaaagcgc cttccacctt caacccgctc tactcgatgg
    16381 atcgtgccgt cgcgcggcgt aacgtcgtgc tatcgcggat gctggatgaa ggatatatca
    16441 cccagcaaca gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg
    16501 agattgcttt ttctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt
    16561 atggcgaaag tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc
    16621 agcaggccgc gcagcaggcg gtacgtaata acgtgctaga ctacgacatg cgccacggct
    16681 atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga
    16741 ttaccgatac gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca
    16801 gcgccaatcc tcaggaagcg acggcaatgc tggcggacgg gtcgaccgtc gcattgagta
    16861 tggaaggcgt tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc
    16921 gtaaagtgac cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg
    16981 catggtggct ggcacaagtg ccggaagtga actcggcact ggtgtcgatc aatccgcaaa
    17041 atggtgccgt tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg
    17101 ccacccaggc actgcgtcag gtgggttcca acatcaaacc gttcctctac accgcggcga
    17161 tggataaagg tctgacgctg gcaagtatgt tgaacgatgt gccaatttct cgctgggacg
    17221 caggtgccgg ttctgactgg cagccgaaaa actcaccacc gcagtatgcc ggtccaattc
    17281 gcttacgtca ggggctgggt cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga
    17341 tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg
    17401 tccacaccga atcgctggcg ctgggttcag cgtccttcac cccaatgcag gtggcgcgcg
    17461 gctacgcggt catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg
    17521 aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg
    17581 atattccggt gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg
    17641 aagatgtcgc tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc
    17701 aggcaaatca ggcgttagtg gcgaagactg gcgcgcagga gtacgcaccg cacgtcatca
    17761 acactccgct ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag
    17821 gctggcaggg taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga
    17881 aaaccggtac cactaacagt tcgaaagatg cgtggttctc gggttacgga ccgggcgttg
    17941 tgacctcggt ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt
    18001 ccggagcgat taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccagcctg
    18061 catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc
    18121 cgccgggtat tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca
    18181 acagccgcga agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg
    18241 tgggaacgac cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct
    18301 tcggcgcctt ttcagtttgc tgacaaagtg cacttgttta tgccggatac gggtgaacgc
    18361 gttatccggc taacaaaatc gtgaaaactc aatatattgc aggaacccca taagcctgat
    18421 aagcattgtg catcaggcaa acttcacgca tttacactcg cccctgccct ttcaaccatt
    18481 cgcgcacgag gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca
    18541 aatccatcat atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt
    18601 ccagcgattc cgggtagaga tcttgcgcta ccacgatatt cattttgctg gaaaagtaag
    18661 acggtgccat gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat ccaacctctt
    18721 cttttagctc gcggttagcg gcttcgaaga cgctttcacc cggatcaatt aatcctttcg
    18781 aaaaacctaa ttcgtaggat tcagttccca ctgcatattc gcggatcagg atcaggtgat
    18841 cgtccacaat cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa
    18901 cacgccgcac gccattgctg aactctagat ccacgctctc gacggtaaac agtcgggaac
    18961 gggctacagt ttcaacattc agaatggtgg gtttttgtaa tgatttgctc atcgtgggat
    19021 ctatgctgtg aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt
    19081 gcatgttatt tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc
    19141 aataggttga aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga
    19201 tcagcggtta ctgtgatgtg cttaatgatg ctcaagttaa actccacgct tgccgatagc
    19261 caaccgcaga atcatgtatt gtgtccggtg cgactgacca cgcctgacag actaagtaag
    19321 atggggaaag catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac
    19381 ttgcgggatg gctgataaaa gtgcgatcca gacggcgtca gctgccctgg accaacgcct
    19441 tcgcggatgc gcaaacgcgt aaactcacac ctgaagaacg tagcgccgtt gaaaattatc
    19501 ttgagagcct gacgcaggta ttacaggtgc ctggcccaac gggagccagc gcggcaccga
    19561 tctctctggc gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacgc
    19621 gttacggcat ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag
    19681 tccacctgcc ccctttctgg gagcagtaca tcaacgatga gaataccgtt gaactgattc
    19741 ataccgattc gctgccgctg gttatttcac tcaacggtca tacgctgcag gagtacatgc
    19801 aggaaactcg cggctatgcc ttgcaacctg ttccgtccac gcaggcgtcg attcgcgggg
    19861 aagagagtga gcaaatcgag ctactcaata ttcgcaaaga aacgcatgaa gaatatgcgc
    19921 tgagtcgtcc gcgcgggctg cgtgaagcgt tgctgatcgt cgcctccttc ctgatgttct
    19981 ttttctgcct gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcgttactgc
    20041 tgctgggcgc aggtctgtgg gggctattcg cccccccggc aaaatcctcc ctgcgggaaa
    20101 ttcattgtct gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac
    20161 agatcaacaa tatttcactc ggtattatcg acctggtcta tcccgcacac tggcagccat
    20221 acattgctca ggatctcggt caacaaaccg atattgatat ctatctcgac cgccacctag
    20281 tgcgtcaggg gcgatatctt tcgctgcatg atgaagtaaa aaactttccg ttacagcact
    20341 ggctgcgcag tacgattatc gctgcgggct cgctgctggt gctgtttatg ctgttattct
    20401 ggatcccgct ggatatgccg ctgaaattca ctctctcatg gatgaaaggt gcgcagacca
    20461 ttgaagccac cagcgtaaaa caactggctg acgctggcgt gcgggtaggc gatacattgc
    20521 gtattagcgg tacgggaatg tgtaatattc gcacttccgg aacctggagc gcgaaaacca
    20581 attcaccttt tttaccgttt gactgctcgc agatcatctg gaacgacgcc cgctcattac
    20641 cattaccaga atctgaactg gtcaacaaag cgacggcatt gactgaagca gttaatcgcc
    20701 agctgcaccc taaaccggaa gatgaatctc gcgtcagcgc ctcattacgt tcagcaattc
    20761 aaaaatccgg catggtattg cttgatgatt ttggcgacat tgtactgaag acagcggatc
    20821 tgtgttctgc caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca
    20881 gtaaagactg ggacgcgctg gtaaaacgcg ccaacgccgg gaagctcgat ggcgtgaatg
    20941 tgttattacg cccggtgagt gcggaatcgc tggataacct ggtggcaacc tccaccgcgc
    21001 cgtttatcac gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg
    21061 gattcctgat tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat
    21121 cactttacga ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc
    21181 tgatgcatac gccgtttaac gccgagggta tcgtcacaaa aatcttcact gacgccaatg
    21241 gtacgcagca tataggcctt catccgatcc cggatcgttc cggcctgtgg cgctatctca
    21301 gcaccacgct gctgctactg acgatgctgg gtagcgccat ttataatggc gtacaggcct
    21361 ggcggcgtta ccagcgtcat cgcactcgca tgatgaagat tcaggcctat tatgaaagct
    21421 gcctgaaccc gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg
    21481 caggttatgc taccctgtcg cgcaaattgc ttcactctgg agattttcct catgcatatc
    21541 aacattgcct ggcaggacgt agataccgtt ctgctggata tggacggcac gttgctcgac
    21601 ctcgccttcg ataactattt ctggcaaaag ctggtgcctg aaacatgggg cgcgaaaaac
    21661 ggggttacgc cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat
    21721 acgctaaact ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg
    21781 atgaccaccg agatgggacc gcgtgccgta ctgcgtgaag ataccattcc gtttcttgag
    21841 gcactgaaag ccagcggtaa gcagcgaatt ttgctcacca atgcgcatcc gcacaacctg
    21901 gcggtaaaac ttgagcatac cggtctggac gcacaccttg atttattact ttccacccac
    21961 acatttggtt atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt
    22021 ctgaaagctg aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg
    22081 caatttggta ttcgttactg cctcggcgtg actaatcctg actcagggat tgccgagaaa
    22141 cagtatcaac gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga
    22201 aggagacgcc atgaaagaaa aacctgctgt tgaggttcga ctggataaat ggctatgggc
    22261 tgcccgtttt tataaaaccc gcgcgctggc ccgtgaaatg attgaaggcg gtaaggtgca
    22321 ttacaacggg cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct
    22381 gcgccaggga aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc
    22441 cgccagcgag gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa
    22501 aatggcgctg gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga
    22561 caaaaaagag cgccgcgacc tgttacgatt taaacacggc gacagtgaat aactgtcacc
    22621 tgcaagagag atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaactt
    22681 tgccgtgcgc ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaatca
    22741 cgattatccg cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt
    22801 aaccgctacg ctgaagtttg atggtgatat caccgtacag ctgcaaggcg acggtccgat
    22861 gaatctggcg gttattaacg gtaacaacaa ccagcagatg cgcggtgtgg cgcgcgtgca
    22921 gggcgaaatt ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat
    22981 caccattacc ccgagcgaag gtgaacgcta tcagggcgtg gtgggtctgg aaggtgatac
    23041 cctggcggcc tgcctggaag attactttat gcgttctgaa cagctgccga cgcgcctgtt
    23101 tattcgcacc ggcgacgtag acggcaaacc ggctgcaggc ggtatgttgt tgcaggtaat
    23161 gcctgcgcaa aatgcccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat
    23221 caaaaccgaa gaactgctga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga
    23281 agaagaggtg acggtttacg atccgcagga tgtggagttc aaatgcacct gctcgcgtga
    23341 acgttgcgcc gatgcgctga aaacgctgcc tgatgaagaa gtcgatagca tcctggcgga
    23401 agatggcgaa attgacatgc attgtgatta ctgcggtaac cactatctgt tcaatgcgat
    23461 ggatattgct gaaatccgca acaacgcgtc tccggcagat ccgcaagttc attaatgagt
    23521 atgtccggca gagagagctc tgccggattt tacgactaat acacctgatg cgctttgtca
    23581 ctcattcaga ttcacatcca gcctttcacc atccactaca agatcgcgcc acgctttagg
    23641 cttcgttccc agttttccat ccttcgcagc tgtgagtaat ttttctttcg ttatatcacg
    23701 cggaagtaaa ctcatcgtaa gctcttgcca ttctttcttg tcaggatcaa agccatagac
    23761 aatgtatctt tcatcatcaa aggcaaagag gatccgctct gccctgccat cactattcag
    23821 atcttgctcg accagtacgc aggcgtcttt ttcaatacag gtcatcacgt tatagcgatc
    23881 ctggattaag gccgaccaga atgtcgcatc cggtttacca gaaccagggg caattaacac
    23941 attttcggct aatacttttt ccgatatctg ttgctgaaga tgttgctctc catctaacgc
    24001 catcagtaga tcccgcgcgc gtttcgggtc tttcatatac tcggcatcgc ttttcagcga
    24061 ctcaagcgcc gcacgtccat agcgaccgct gtgttcgagc atgtagatag ttacctggtc
    24121 tggcgtgttt ttgccgctct gataacgcgc catatgactg ttcacactaa tgcgcatact
    24181 gtccagcacc ggcgaattaa gaagcaccag tattaccaac accaataacg aaaccgcaag
    24241 gttcactttg ccctgaagaa caaggggatt ttgcccttta cgccagacga tactgacaaa
    24301 ataccccagc gaccatacca gtaacaccag caccgccagc gcgccctgca agcggtcgac
    24361 agtccagccg tactgagcga cccgcagcca taacgcccag gcggcgatga acacatacag
    24421 cggagcaacc agcagagcgg ttttaatcag gcaacgcaac ggccccgtcc agggaagtga
    24481 cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg ccagcgttaa
    24541 cagcaacccg gcggcggaga tgtggcgaga aatcgcactc aggcctgtaa acggcagggt
    24601 gatgataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg cgattaacgt
    24661 gaacaacttt tgaatagagt cgattaaacg tgactgtgtt cgcgcgagga tcaccgccag
    24721 cgcggtaacc agacctaacg tgagataaat aaaccagtcg gttgcaaaaa agagcgtttt
    24781 gaaaaacgtg atgccaacaa gtttaaacaa ctcactccac agtaaaagca ccagccacgt
    24841 cagaccattg gcgagaaaaa tcaccagtaa tataagtacg ttatgccata ctgactggta
    24901 aaaatagcga tagcggctac tgtcattgcg gatgcgaagg ctttgttgta tccacggtag
    24961 caacaacatt gccatcagca gcagatagca accaaaatcc caaagcgcct tttcagctct
    25021 ccagggggtc atgccatcag tttgccactt cagccagccg ctcatcccca gcgtggcaat
    25081 aaacaccagc gccagccatc cccaaaggcg cttctgttta aaagagatca cggagaagag
    25141 taaaacggat gaaaacgcga cggtcgcagg cacgccataa acaatccaac tgtgattttt
    25201 tccggaaagc caggcgatca gcaggtagca aaccagaccc tgaagtaatc cggtagcaat
    25261 catcccccag cgtgttgcgg gtgaaagttc aacgttatcc atgttggtta tccagaatca
    25321 aaaggtgggt taattatcgc atccgggcag tagtattttg cttttttcag aaaataatca
    25381 aaaaaagtta gcgtggtgaa tcgatacttt accggttgaa tttgcatcaa tttcattcag
    25441 gaatgcgatt ccactcacaa tattcccgcc atataaacca agatttaacc ttttgagaac
    25501 attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg acaggaatca
    25561 cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc cttgtcgcgg
    25621 ttaacccccc caaaaagact ttactattca ggcaatacat attggctaag gagcagtgaa
    25681 atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat cagtgacgta
    25741 catgatatcg tttacaaccc aagctacgac ctgctgtatc aggaagagct cgatccgagc
    25801 ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt cgataccggg
    25861 atcttcaccg gtcgttcacc aaaagataag tatatcgtcc gtgacgatac cactcgcgat
    25921 actttctggt gggcagacaa aggcaaaggt aagaacgata acaaacctct ctctccggaa
    25981 acctggcaac atctgaaagg cctggtgacc aaacagcttt ccggcaaacg tctgttcgtt
    26041 gtcgacgcct tctgtggtgc gaacccggat actcgtcttt ccgtccgttt catcaccgaa
    26101 gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga tgaagagttg
    26161 gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa cccgcagtgg
    26221 aaagaacagg gtctgaattc cgaaaacttc gtggctttta acctgaccga gcgcatgcag
    26281 ctgattggcg gcacctggta tggcggcgaa atgaagaaag ggatgttctc gatgatgaac
    26341 tacctgctgc cgctgaaagg tatcgcttct atgcactgct ccgccaacgt tagcgagaaa
    26401 ggcgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct ttccaccgac
    26461 ccgaaacgtc gcctgattgg cgatgacgaa cacggctggg acgatgacgg cgtgtttaac
    26521 ttcgaaggcg gctgctacgc aaaaactatc aagctgtcga aagaagcgga acctgaaatc
    26581 tacaacgcta tccgtcgcga tgcgctgctg gaaaacgtca ccgtgcgtga agatggcact
    26641 atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc gatctatcac
    26701 atcgataaca tcgttaagcc ggtttcaaaa gcgggtcacg caacgaaagt tatcttcctg
    26761 accgcagacg cctttggtgt gctgccaccg gtttctcgtc tgactgccga tcaaacccag
    26821 taccacttcc tctctggctt caccgccaaa ctggccggta ctgagcgtgg catcaccgaa
    26881 ccgacgccaa ccttctccgc ttgcttcggc gcggcattcc tgtcgctgca cccgactcag
    26941 tacgcagaag tgctggtgaa acgtatgcag gcggcgggcg cgcaggctta tctggttaac
    27001 actggctgga acggcactgg caaacgtatc tcgattaaag atacccgcgc cattatcgac
    27061 gccatcctca acggttcgct ggataatgca gaaaccttca ctctgccgat gtttaacctg
    27121 gcgatcccaa ccgaactgcc gggcgtagac acgaagattc tcgatccgcg taacacctac
    27181 gcttccccgg aacagtggca ggaaaaagcc gaaaccctgg cgaaactgtt tatcgacaac
    27241 tttgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggctgg tccgaaactg
    27301 taatgatttg aagctggaga atatctatcc agtatcttat agaaagcaaa acgggaggca
    27361 ccttcgcctc ccgtttattt acccttcttt tgtcgtgctc tgcgcccgcg ttaccggcac
    27421 aggcagccag gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc
    27481 gttatggtta tccacgatac gctgcacaat cgccagccct aatcccgtgc cgctaatggt
    27541 gcgtgcactg tcgccgcgaa caaacggctg gaaaaggtgc ttacgttgtt ccggcgcaat
    27601 tcccggaccg tcatcttcca cctggaacca ggcgcgattc ggctccgtcc cgctgctgac
    27661 tttgatccag ccattgccgt aacgggcggc gttgaccacc atattcgcca ccgcgcgttt
    27721 gatcgacagc gggtgcattt tcacttcaat gctgccgggg taaagcgcgg tttcaatttc
    27781 ccgctcatag ccactttcgg cagcaataac ctcaccgagt actgcgttga gatccgccat
    27841 ttccatcggc atctcctgtc cggtgcgcag gtagtcgata aactgctcaa tgatggcatt
    27901 gcactcctcg atatctttat tgatcgattc tgccaggtag ccatcctgct cgctcatcat
    27961 ctcggtcgcc aggcgaatac gcgtcagcgg tgtacgcaag tcgtgactta cccccgccat
    28021 cagcagcgtg cggtcatccg ccagttgctt aacaccagcc gccatatggt taaaggcgcg
    28081 ggtaacggaa cgcacctcgg acgcgccata ctcacgcagc ggcggcggaa taatcccttt
    28141 accaacctgc aaggctgcgt gttcgagatc gaccaacggt cgattctgga tacgaataaa
    28201 cagccacgcc ccgcctatcg ccaatagcat aatcgccaac gtatagcgga acagcggaga
    28261 gaaatcgccc tgatgaattt cggtcagcgg cacgcgtacc cagatattgg gcgacagcca
    28321 ggttttcagc cagacgacag gcgaactttt gttgacctca acgcgcactt ccgtcgggcc
    28381 gcccagttgc tgtgccatct gatggcttaa gaattcatag tgttgcgccc aacgcagacc
    28441 tgcctcttcg gcagcctcgt tggagtagag agagatcccc agctcacggt agatctcccg
    28501 acggaaagcg ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat
    28561 caacatacgc acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc
    28621 gaagttcagc accaccagat aagtcgtcac caggctggcg aacagcaagg tgacgatgag
    28681 caataacgta cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagag
    28741 ccgtccggta caaagacgta gcccaggccc caaacggtct gaatgtaacg cggatgcgct
    28801 ggatcttctt ccaccatgcg gcgcagacga gaaatctgca cgtcgatgga gcgttccatt
    28861 gcggaatatt cacgaccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc
    28921 ggatggctga ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg catcggctcg
    28981 tcttcgcgga acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc
    29041 gcctcttcct gtgacggtgc gcctggtagc tcgttcgcct gacgacgcag caccgcacgg
    29101 atacgggcca gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc agcgccaatc
    29161 tccaggccaa cgatacggtc cacttcttcc cctttcgccg tcaccataat gatcggcatc
    29221 gggttgctct gactacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatt
    29281 aaatccagta ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca
    29341 ttagcgacgc ttcgaacctg gaagccttgt tcggtgagat aacgttccag cagcgcacgc
    29401 aggcgcatgt cgtcatcgac caccagaatc ttgtagttct cttgcattgt ttgtactccc
    29461 aaaggttcgc aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct
    29521 aaatctggta tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta
    29581 tacaatttat tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt
    29641 gcgcattatc aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga
    29701 agggtatgaa aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt
    29761 cacaaaaaag gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca
    29821 gtataataaa aagcgcctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat
    29881 ggaaaatctc aaaatcgtcg attactcccc gcagcaggaa ggcaaagtct tttttggcgc
    29941 gtgggtggag attgaaaacg acgatggcgt gactcaccgt ttccgtattg tcggctacga
    30001 tgaaattttt ggccgtaaag attacatctc tatcgattcc ccgatggccc gcgcattgct
    30061 gaaaaaagaa gtcggcgatc tggctgtggt gaatactcct gccggggaag cgagctggta
    30121 tgttaatgct atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct
    30181 ggcatttttg ccagccagtc cgtataacta tcccctgatt tttgatccga aaagatgaac
    30241 tcaaaccatg atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc
    30301 ggaacaggtt gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cttttattgc
    30361 acgttatcgt aaggaaatca ccggcggtct ggatgacacg cagctgcgta atctggaaac
    30421 gcgtctggga tacctgcgcg agctggaaga gagacgtcag gcgatcctca agtccatttc
    30481 cgagcaaggc aaactcaccg atgatctggc gaatgccatc aacgccaccc taagcaaaac
    30541 cgaactcgaa gacctctacc tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc
    30601 cattgaagca gggcttgagc cgttggctga cctgctgtgg agcgatccgt cacacacgcc
    30661 agaagtcgcc gctgcgcaat atattgatgc cgataaaggc gtggcagata ccaaagccgc
    30721 gctggacggc gcgcgctata tcctgatgga acggtttgcc gaagatgccg cgctgctggc
    30781 gaaagtgcgt gattatctgt ggaagaacgc gcatttggtt tctacggtgg tgagcggtaa
    30841 agaagaggaa ggggcgaaat tccgcgacta tttcgatcat cacgaaccgt tgtccacggt
    30901 gccttctcac cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac ttcagctttc
    30961 gctgaatgcc gatccacagt tcgatgagcc gcccaaagag agctattgcg agcaaatcat
    31021 catggatcac cttggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt
    31081 ggtgagctgg acgtggcgca tcaaggtgct gatgcatctg gaaaccgaac tgatgggcac
    31141 cgtgcgcgaa cgtgcggaaa atgaagcaat caacgtcttt gcccgtaacc tgcacgatct
    31201 gctgatggcg gcccctgccg gactgcgtgc aacgatgggc ctcgatccgg gtctgcgtac
    31261 cggggtaaaa gtggcggtgg tcgatgccac tggcaaactg gtagcgaccg ataccattta
    31321 cccgcacacc ggacaagccg caaaagcagc gatgactgtt gctgcgctgt gtgaaaagca
    31381 taacgttgaa ctggtggcga tcggcaacgg tacagcttcc cgcgaaaccg aacgtttcta
    31441 tctcgacgtg cagaagcagt tcccgaaagt gaccgcacag aaagtgatcg tcagcgaagc
    31501 tggcgcgtcg gtttactcgg cttccgagct ggcagcgcag gagttcccgg atctcgacgt
    31561 ttcgctgcgt ggcgcggttt ctatcgcccg ccgtttgcag gatccgctgg cggagctggt
    31621 gaaaatcgat ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca
    31681 actggcccgc aaactggatg cagtagtaga agactgcgta aacgccgttg gcgtcgatct
    31741 caacaccgct tctgttccgc tattaactcg cgtggcgggc ctgacgcgaa tgatggcgca
    31801 aaacatcgtg gcctggcgcg atgagaacgg ccagttccag aaccgtcagc aactgctgaa
    31861 agtcagccgt ctggggccga aagccttcga gcagtgcgcg ggcttcctgc gcattaacca
    31921 cggtgacaac ccgctggacg cctctaccgt tcacccggaa gcctatccgg tggtggaacg
    31981 cattctggca gcaacacagc aggcactgaa agatctgatg ggtaacagca gcgaactgcg
    32041 taacctgaaa gcgtctgact ttactgatga aaaattcggt gtgccgacag taactgacat
    32101 catcaaagag ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt
    32161 tgccgatggc gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggcgctgt
    32221 caccaacgtc accaactttg gcgcgtttgt cgatatcggc gtgcatcagg acggcctggt
    32281 tcacatctct tcattgtcga acaagtttgt ggaagatccg cataccgtgg tgaaagcggg
    32341 cgacattgtg aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac
    32401 tatgcgtctg gacgagcagc ctggcgaaac caactctcgt cgcggcggcg gtaacgatcg
    32461 cccgcaaaac aaccgcccgg cagccaaacc acgcggtcgt gaagcgcagc ctgccggtaa
    32521 cagcgcgatg atggacgcgc tggcggcagc aatgggcaaa aaacgttaaa cgcccggact
    32581 ggcccctaaa cctgcgcgat aaggcgttca cgccgcatct ggcaacgaac gccttgcctg
    32641 acataaaagt gccggagaat atctccggca tatttattcc acagccaaac tcacaatata
    32701 tttcgttaat atttatcact tcattaacaa ctgaaacctt aattaaacat tagccagtcc
    32761 tggtaattcg ctattcgaat tatattttcg ctgcgatata accttgagcc acatcaacat
    32821 tgagtcagat tattattcaa accaacattc gcacacattt taagtattgc tgatagaaac
    32881 cattctcatt atcattgtac tattgattat ttaatctctc cttcgttggc aaatcatctg
    32941 gtctcatgtc gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag
    33001 gcacctatgc aatacactcc agatactgcg tggaaaatca ctggcttttc ccgtgaaatc
    33061 agcccggcat atcgccaaaa actgctttct cttggcatgt tacctggctc ctcttttaat
    33121 gtggtgcgcg tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg
    33181 gtattacgca aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt
    33241 gataacaaca atgaaaaaat taaccatagg cttaattggt aatccaaatt ctggcaagac
    33301 aaccttattt aaccagctca ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac
    33361 cgtcgaacgt aaagaagggc aattctccac caccgatcat caggtcacgc tggtggacct
    33421 gcccggcacc tattctctga ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc
    33481 ctgtcactac attttgagtg gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa
    33541 ccttgagcgt aacctgtacc tgacgctaca actgctggaa ctcggcattc cctgcatcgt
    33601 ggcactgaac atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct
    33661 gtcggcgcgt cttggctgcc cagtgatccc gctggtttca acccgtggtc gcggtattga
    33721 agcgctcaag ctggcgattg atcgctataa agctaacgag aatgtggaac tggtgcatta
    33781 cgcacagccg ctactcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc
    33841 gctgaaacaa cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc
    33901 ctacgccggt gaagcgtcgc agcatcttga tgccgccctc gcccgtctgc gtaatgagat
    33961 ggacgatccg gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg ccatctgtga
    34021 tgtggtaagc aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat
    34081 cgtgctcaac cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttcct
    34141 gctggctatc aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtggctct
    34201 atttgtgcat ggtattcaat ggattggcta cacgctccac ttcccggact ggctgactat
    34261 cttcctcgcc cagggcctgg gtggcggtat taacaccgtg ctgccactgg tgccgcagat
    34321 cggcatgatg tacctgttcc tctccttcct tgaggactcc ggctatatgg cgcgtgcggc
    34381 gtttgtgatg gaccgcctga tgcaggcgct gggcttgccg gggaaatcct ttgtgccgct
    34441 gatcgtcggt ttcggttgta acgtaccgtc ggtaatgggt gcacgtacgc ttgatgcacc
    34501 gcgtgaacgt ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc
    34561 tatcttcgca gtattcgcgg ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc
    34621 gctgtatatg ctcggtatcg tgatggcggt gctgactggc ctgatgctca agtacaccat
    34681 catgcgcggt gaagcgacgc cgtttgtcat ggagctgccg gtctaccatg taccacacgt
    34741 taaaagcctg attatccaga cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa
    34801 agtgattatc atcgtcagca ttttcctgag cgctttcaac agcttctcgt tgagcgggaa
    34861 aatcgtcgat aacatcaacg actcggcgct ggcttccgtc agccgggtga tcaccccggt
    34921 cttcaagccg attggcgtgc atgaagataa ctggcaggca acagttggcc tgtttacggg
    34981 tgcaatggcg aaagaagtgg tggtgggtac gctcaacacc ctctacaccg cagaaaatat
    35041 tcaggacgaa gagttcaatc cggcggaatt taacctcggt gaagagttgt tcagcgcggt
    35101 agatgaaacc tggcagagcc tgaaagacac cttcagcctt agcgtactga tgaaccccat
    35161 tgaagctagc aaaggcgacg gcgaaatggg taccggggcg atgggcgtga tggatcagaa
    35221 attcggtagc gcagctgccg catacagcta cctgattttc gtcctgctgt atgtaccatg
    35281 tatctcggtg atgggagcca tcgcccgtga atcaagccgt ggctggatgg gcttctccat
    35341 cctgtggggg ctgaatatcg cttactcact ggcaacattg ttctatcaag tcgccagcta
    35401 cagtcagcac ccaacttaca gcctggtgtg cattctggcg gttatcctgt ttaacatcgt
    35461 ggttatcggt ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg
    35521 caagtcggta agtagttgct gcgcagccag caccaccggt gattgccatt aatggcttca
    35581 cttattcagg tgcgtgattt gctggcgtta cggggccgta tggaagcggc ccagataagc
    35641 cagacattga acactccaca gccaatgatt aacgccatgc tgcaacaact ggaaagtatg
    35701 ggcaaagccg tgcggattca ggaagaacct gacggctgcc tctccggcag ttgtaaaagc
    35761 tgcccggaag gaaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc atactccatc
    35821 gcctgttttg aaaagcccgg tatgcgtttg catccgggct tttttgcgtg cggctttcca
    35881 taaaaatgca attcttgcag cgcgacgtaa gttcctctga aagcgtctcg cagggatgaa
    35941 aactggctaa tgcacaggtg tggagtggcg cgtagagtcg cggcattcaa acaacaggtg
    36001 aaggaacgcc atgagcaaaa agcagagttc caccccacac gatgcgctgt tcaaactctt
    36061 tttacgccaa ccggagacgg cacgcgattt tcttgcgttt catttaccag cacccattca
    36121 cgcgctctgt gatatgaaaa ccctcaagct ggagtcgagc agctttattg atgacgatct
    36181 gcgtgaaagc tattccgatg tgctgtggtc ggtgaaaaca gaacaaggac caggatacat
    36241 ctattgtctg attgaacatc aaagcacctc aaacaaactg atcgcatttc gcatgatgcg
    36301 ttacgctatt gccgcaatgc aaaatcacct tgatgccgga tacaaaacgt tgccgatggt
    36361 ggtgccattg ttgttttacc acggtattga aagcccctat ccctattcgc tgtgttggct
    36421 ggattgtttc gccgatccca aactggcaag gcagctttat gcctccgcat ttccgctgat
    36481 tgatatcacc gtcatgcctg atgatgaaat catgcagcac cgacgcatga cgctgctgga
    36541 gttaattcaa aaacatattc gtcaacgcga cctgatgggg ctggtagagc aaatggcctg
    36601 cttattaagt agtggatacg ctaatgacag acaaatcaaa gggctgttta attacatact
    36661 gcaaaccggc gatgcggtac gttttaacga ttttatcgac ggcgttgccg aacgatcacc
    36721 gaaacacaag gagagtttaa tgactattgc ggaaagattg cggcaggagg gggaacaatc
    36781 caaagccctg catatagcca aaataatgct tgaatccgga gtccctcttg cagacatcat
    36841 gcgctttacc gggctgtcag aagaagagtt ggctgcggcg agtcagtaaa gttctgtctc
    36901 gccattcaaa agtgcctaca ccctctgctt caacgccacc agcacgtgac gaaactcgac
    36961 cggatgcgaa ataaacggcg catgagccgc tttggcgaag atgtatgact cgctgtgtgg
    37021 ccagagttta tccagcatcg gcaccacttt gcgcggcacc agaccatcga gatagccata
    37081 cagtcgcaaa aacggcatgg gtacgttttg cagcggcaag cggagatcaa ccgttttgag
    37141 gatttccagc ccaccattga gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt
    37201 cttcaacgcc cgcgcatcct gacgcgccgt ttcagtaccc atggtttgta acgcaaggaa
    37261 ccgttccacc gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc
    37321 cggttttatc cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac
    37381 cagcgcctga acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag
    37441 actccagcct aaccaaatgg ctttatcagg tgcctgtcgc agcacggctt cggccatctc
    37501 cgcaagtgac agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaagatg
    37561 cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcag cattcagtcc
    37621 ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt
    37681 attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg
    37741 attatgctgg ctatgccgaa tgccactggc attaggtcat tgggggattt gttcggtctg
    37801 ctcacgcgcc gcccgtacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca
    37861 ctcccatctt ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac
    37921 ggttgccgac tatgcgccgc cgttaagtct gctcatccac cagcttaagt tttcccggcg
    37981 cagcgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg
    38041 caccaccggg ttgcaattgc cggatcgtat catcagcgtt ccgttatggc agcggcgtca
    38101 ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca
    38161 ctgccaatgg gatagcgaag ccatcacacg tacacgggcc actgcgaccc agcattttct
    38221 tagcgcccgg ctacgcaggc gcaacctgaa aaatgccttt cgccttgaat tgcctgtgca
    38281 aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat
    38341 tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac
    38401 cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca
    38461 ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc
    38521 aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat
    38581 gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacaccgcc ctgaaatttg
    38641 acctgctgac cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg
    38701 attttgttac cgaccagttg ggttctcagc taacactgaa agcgcctaac gccaaaatgc
    38761 gtaaagtggc agacgatgca ccactgatgg agcgcgtgga gtatatgctg cagtcgcaga
    38821 tcaacccaca gcttgctggt cacggtggcc gcgtttcact gatggaaatc accgaagacg
    38881 gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc
    38941 tgaaagaagg gatcgagaag cagctactaa acgaattccc ggagctgaaa ggtgtgcgcg
    39001 atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag
    39061 attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt
    39121 ggcgcagcat tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct
    39181 ctcataattt aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca
    39241 ttcacgtggg taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta
    39301 tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagct tgtcgtcaga
    39361 aaattgacgt tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg
    39421 ctatcggtgt aatcttgtta ttactcctga tgatccgctt caaaatgaac ggcttcatcg
    39481 ctctcgtcct cgtggcgctt gcggttggat taatgcaagg gatgccgctg gataaagtta
    39541 ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt
    39601 ttggcgcaat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca
    39661 cgctgattgc caaatttggt aaaaaacaca tccagtgggc ggtggtactg accggtttta
    39721 ccgttggttt tgccctgttc tatgaagtgg gctttgtgct gatgctgccg ctggtgttta
    39781 ccatcgcggc ttctgcgaat attccactgc tgtatgttgg tgtaccaatg gcagctgcac
    39841 tgtctgtgac ccacggcttc ctgccaccgc atccgggtcc gactgcgatt gccaccattt
    39901 tcaatgccga tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga
    39961 ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat cgataagcca attccggaag
    40021 gtctctacag cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct
    40081 ggacctctct ggtgccggta gtactgatgg cgatgcgtgc gattgccgaa atgatcctgc
    40141 cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc
    40201 tgattgccgt gctgattgcg atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc
    40261 agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg
    40321 gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt
    40381 ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac
    40441 tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac
    40501 cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtg ggttccggta
    40561 gtgtgatttt ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc
    40621 tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg
    40681 gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat
    40741 cgcatccggc atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca
    40801 aatccttcag caacttgttc acgccatcat cggcaaacat cgtctcaagc gttgcggaaa
    40861 gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca
    40921 tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc
    40981 gctgcaaacc acgattaagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac
    41041 gttttggcag acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc
    41101 gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca
    41161 gcgttagatc cccgctctcc cagtagccgc gtagcgtagg caggtcatgt gtcgccgcaa
    41221 ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt
    41281 cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta
    41341 ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg
    41401 ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct
    41461 cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc
    41521 cgcagttttg catattggca cgcaacagct cgataaacgg ttcataagca cgcgcggtga
    41581 tgatatgcgg gtccattggc ggcaatcccc agttctgccc caacgggccg aggatatccg
    41641 gcggcgcgcc aaccgacgct ttcaggcaat acagttcacg gtcgcaccag gtttccgccc
    41701 caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct
    41761 ggcttatctc ccagcaggcg gcaaactggc tgtaagcctg ccactgcaac cagagataaa
    41821 aatcaacgtc atcgcgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct
    41881 gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg
    41941 catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccttgatct gcaacaaact
    42001 ggcgaaacgc ggccatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta
    42061 atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcccgcgcct
    42121 gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat
    42181 ggaaatcttc gacggcgtta acgtcgatat aaatcacatt cagccagcgg cgagaagacg
    42241 ggctatacgg gctggcgctc tccggatttg ccggatagag cgcatgaatc gggttcagac
    42301 caatgaacga tccgccacgt tttgccacat ccactagcat cgctttgaga tcgccaaaat
    42361 ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctggacgcag gcaccccaca
    42421 gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc
    42481 tgcaatgcgc gcgctggtct tcctgggtaa gcgtcagcgt gtgataacct tccggcagct
    42541 tcgtcggtag attgaacgct ttgccccccg ttatatggcc tttgtactgc gttccttctt
    42601 cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc
    42661 cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt
    42721 gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt
    42781 gggcattgat gtaatgaggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc
    42841 tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag
    42901 agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg
    42961 cctcctggtc gcggtacagc acatccacct gcttttgtgc ctctacatag gctgcgaaat
    43021 ccgccatcac cagatacgga tcgccgccct gtttgccgat actgtgcagc atctggtcga
    43081 aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca
    43141 gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga attgccttca
    43201 cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt
    43261 caacgttcgc cccatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat
    43321 tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg
    43381 ggatcagttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat
    43441 cgccaaccag cggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat
    43501 ttttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc
    43561 gatcagcctg tgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca
    43621 ggtgctggcg tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat
    43681 tgatctcaat accggtacga actttcacaa actccgccag acggacttta ttcgcctgct
    43741 tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca
    43801 gctgatcgag atcgttagcc cactcttttt tcagtgattt atccaacaga gccgccagtg
    43861 ccgggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt
    43921 tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca
    43981 gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt
    44041 gtttgtcgtg caccaccgcc agtttagccc acactttttc atcgcccggc caggttttct
    44101 ccaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatgtgg cgcggcagta
    44161 agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg
    44221 tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc tggtgctcat
    44281 cgatcagcac gcgcaacagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa
    44341 taacttcgta atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa
    44401 tatccgctac cgaacaggca cactggaagt actgctgcat caagcgcagc tttttaccgg
    44461 cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccctgct
    44521 gttcggcacg caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcgtgcg
    44581 tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat
    44641 cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac
    44701 cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc
    44761 gatgccagtc atccggcgct tcaacctgtt tgccatccac aaaagactgg cggaacaaac
    44821 catattgata gttcagaccg taacccgtcg cagactgacc gacagttgcc attgaatcga
    44881 ggaagcacgc cgccagacgt cccagaccac cgttacccag cgcagggtcg atctcttctt
    44941 ccagcaggtc agtcagattg atgtcataag ccttcaacga atcctgtaca tcctgatacc
    45001 agccgagatt caacaggttg ttgcccgtca gacgaccaat caaaaactcc attgagatgt
    45061 agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc agcatttcgg
    45121 ccagtgcttc actcactgcc agccaccact ggcgaggagt catttcagcc gcagaattta
    45181 agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag
    45241 gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc
    45301 caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga
    45361 tgagcaagga aatgtgatct cgaccactta aagctagtgc aaaccacagg attagcagca
    45421 aatcaatgcc atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat
    45481 tttcaaaaag cgaaaggtaa ctctataaat taagtaaagg agtgaaacag tttcacaagt
    45541 aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat
    45601 agcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg
    45661 gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga
    45721 attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt
    45781 ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt
    45841 tccacagtga agtgattaac tatgctgatt ccgtcaaaat taagtcgtcc ggttcgactc
    45901 gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg
    45961 ctggcgctga tcacaagtcc tgcgggctac ggtaagacca cgctcatttc ccagtgggcg
    46021 gcaggcaaaa acgatatcgg ctggtactcg ctggacgaag gtgataacca gcaagagcgt
    46081 ttcgccagct atctcattgc cgccgtgcaa caggcaacca acggtcactg tgcgatatgt
    46141 gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc
    46201 attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc
    46261 actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc
    46321 accctggtgg tgttgtcacg taaccttccg caactgggca ttgccaatct gcgtgttcgt
    46381 gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag
    46441 ttttttgatt gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg gatttgcgat
    46501 gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc
    46561 cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat
    46621 tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa
    46681 agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg cgaagaaaac
    46741 gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat
    46801 accggcgagt ggttctgcta tcacccgctg tttggtaact tcctgcgcca gcgctgccag
    46861 tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc
    46921 cagggattcc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg
    46981 cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg
    47041 gaagagtcgc ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttggtgtta
    47101 ttgcaggcgt ggctgatgca aagccaacat cgctacggcg aagttaacac cctgctggcc
    47161 cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct
    47221 ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa
    47281 ctggcactgg aagagctacc gccgggctgg ttctatagcc gcattgtggc aacctcggtg
    47341 ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa
    47401 accgaacaga tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaattcag
    47461 caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac gcaggaaaaa
    47521 gcgttccagc tgatcaacga gcagcatctg gaacagttgc cgatgcatga gtttctggtg
    47581 cgcatccgag cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg
    47641 cgtagcggga ttgaagtctt atcgtcttat cagccacagc aacagcttca gtgcctggca
    47701 atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac
    47761 cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac
    47821 aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg
    47881 cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac
    47941 attgcccgcg cacaaatctt gctgggcgag tttgaacctg cggaaattgt cctcgaagaa
    48001 ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg
    48061 ctaaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac
    48121 gcattaaaac tggcgaatcg caccggattt atcagtcatt ttgtcatcga aggcgaagcg
    48181 atggcgcaac aactgcgcca gctgattcaa ctcaatacgc tgccggaact ggaacagcat
    48241 cgcgcacagc gtattctacg agaaatcaat caacatcatc ggcataaatt cgcccatttc
    48301 gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact gatccgcacc
    48361 agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaac
    48421 gaacaaatag cgggtgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat
    48481 ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg
    48541 ctgaagatga tgggttatgg ggtgtgagtt tgtatagcgt ttttatgtac actctttata
    48601 ctgtatataa aatcgcacta caggctcatg aatatgaaac cggaaagcaa agaagcgcca
    48661 atcaatatca gagcgaaagc ctcacagcgt gacctgattg atatggcagc gaatctggtc
    48721 gcaaaatcgc gcactgattt tatgctggat gccgcatgcc gtgaagcgca agacatctta
    48781 ttagatcaac gactattcat ccttgatgac gagcaatacg atgcgtttct cgctgccctg
    48841 gacgccccca tcactgctga gcgtcaggcc aaaattaacg cgttgatgaa tcgtaaatcg
    48901 ccatgggaat aacggctcct actcccctaa cgtctgaaca taatctggct gacttttgct
    48961 gttccgatca cgggatgaat gaatggctaa aaaagaaagc gttaaaaaat cacagctccg
    49021 gtctgtctcg tgtttacgtt atctgtattg ccaatacccg ccaggtaatc ggctattact
    49081 gcctttctac aggcagtatt caacgtaatt tggcccctgg agccatgcga cgtaatgctc
    49141 ccgaatcgtt gcctgttgtc gtattaggca gactggcaat tgatcaagcc tgggcaggta
    49201 aagggttggg cgtcgcattg ttgaaagatg cagtctatcg cacaatgtcc atagctcaac
    49261 aggtaggggt tcgtgcgctc attgttcatg ccttagacga ttcagtacgc aatttctatc
    49321 tgaagtatgc ctttgtacct tcaccattcc agtcacttac cttactgtat cccattactc
    49381 tggagtaatt tacctctacc ggatagtcac actgatgagt ctgatccggg agggatttca
    49441 atcagtgagt ttggttatca tcacccgcgt tacgccatct gtttcagcca gagtaaaccg
    49501 caccggcaag aaacgctcca ccacggcgat attggtcagc agatggcatg agggatgggc
    49561 gaccgtaaat tgtcccgcgc ccgccagcgc cattggcaac actagctggt cagcaagata
    49621 ttcccccact gccgccgggc ttgccaggta gcgtttcacc tctttcacca actgtgccgc
    49681 aaccacctcg gcactgacgc gcttttcacc gacgacaaaa aagcgttcgg tgatattttc
    49741 actttcgact tcaagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat
    49801 attctgctca tgcagggaaa aacttgccgc cagcgtagcg atttcacgct cagcgacatg
    49861 ccgcggcacg ccagctaata acacctctcc acgcatctgc acaatgttcc cgcgctcgcc
    49921 aagttgcaag gtgttaaacg atgccaccgg cgagacttcc gttgccacca cgccgcctcc
    49981 ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag
    50041 cagcggctcc aacacccggc ggataaaatc cgcaggcggg gccgacgggt tatcggtacc
    50101 gccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg
    50161 cagcaccagc gtacaacttc ctgcgctacc gatagcaaag cgataatcgc cgccacgcac
    50221 ggtgccgggc cggaagacca gacgctgcga ccccagctcc gcgccttcca ccgtcgcccc
    50281 gcaaatttcc gtcgccgcct ttaccgccgt cagatgctgg cgcaacagtc ccggttttgc
    50341 ccgcccggca cgaatgccgg tgatggtaaa tggctggccg gttatcatcg acagactcag
    50401 cgccgagcgc atgatctgcc cgccgccttc gccctgtgcg ccatccagcg caatcatcct
    50461 tttcatcatt tatcctttta cgcacaccac ctgacgtagg gtgtagataa cttccaccag
    50521 atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat
    50581 cacttcggca tctttacggc attccacatg cacggtggca cgaatttgat cttccacgct
    50641 gaacattttt ttcgctttag tacggctcat aacccgcccg gcaccgtggc tgcacgaaca
    50701 gaacgactct tcatttccca gcccacggac gataaagctt ttcgctccca tcgaccctgg
    50761 aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc
    50821 ttcaccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc
    50881 cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttactacgtt
    50941 ttccatcatc acatcgcggt taaggctggc aaaaagctgc gcccaggcca cggctttcag
    51001 gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg acggcaatgt
    51061 ttcaagcgtt tcctgcatct ctttttgcgc cagatcgata aagtaagtcc cgatggcatt
    51121 accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact catcaaggca
    51181 gatttcaata aagtggttac ccgttcccag cgttcccagg tgtttatagt tgttagtgtt
    51241 aaggaaacgc ggatattttt gcgttaacca ctgataaccg gcctccagct cagcccattt
    51301 cgcatcgacg ttaacaggcg ggttttccca tgcgccttta tcacgtttac aacggccagt
    51361 ggtacgcccg tgcggcaccg cagtttcaat cgcctgacgc agctccgcca ggttttccgg
    51421 caggtcagcc gccgttaaag cggtacgtag tgcgttcatt ccacagccaa tatccacgcc
    51481 caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt
    51541 acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt
    51601 attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac
    51661 cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt
    51721 gcggtcctga taattgcaac cgtcgtgcca gaaaattaaa taagcagatt aattttttaa
    51781 ttcattgttt taaaaagatt atatctttac gtccgtaaac ggagatttcc cgcaaagcca
    51841 atttaccgat aatgaaatat cgtcttttat aaggatatct aagatgcgta aaacagtggc
    51901 ttttggcttt gtcggaacag tgctggatta tgccgggcgc ggcagtcagc gctggtcgaa
    51961 atggcgtccg tcactctgta tatgccagca agaatcgtta gtcatcgatc gactggaatt
    52021 attgcatgac gcccgctcgc gctcgctatt tgaaacgctt aaacgcgata tcgccagcgt
    52081 ttcgccagaa accgaagtgg tgagcgttga gattgaactg cataacccgt gggatttcga
    52141 agaggtttac gcctgcctgc atgatttcgc ccgtggttac gagtttcagc cagaaaaaga
    52201 agactattta attcacatca ccaccggcac ccacgtcgcg cagatttgct ggtttctgct
    52261 ggctgaagcg cgttacctgc ccgcccggtt gatacaatct tcacctccgc gcaaaaaaga
    52321 acagccgcgc ggcgcaggtg aagtgacgat tatcgatctc gatttaagcc tttataacgc
    52381 catcgccagc cgctttgccg aggaacgcca gcaaacgctt gattttctta agtccggtat
    52441 tgccacgcgt aacccccact tcaaccgcat gattgagcag attgaaaaag tagcgattaa
    52501 atcccgcgca ccgatcctgc taaacggccc aaccggtgcg ggcaagtcat ttctggcgcg
    52561 acgtatattt gaattaaaac aggcgcggca tcagtttagc ggcgcgtttg tggaagtgaa
    52621 ctgcgcaacc ctgcgcggcg ataccgccat gtcgacgctg tttggtcatg tgaaaggcgc
    52681 gtttaccggg gcacgggaat ctcgtgaagg attattacgc agcgccaacg gcggaatgtt
    52741 gtttcttgat gagattggcg aactgggcgc agacgaacag gcaatgctgc tgaaagccat
    52801 tgaagaaaaa accttttatc cgtttggcag cgatcgccag gtgagcagtg attttcagct
    52861 tatcgccgga acggtgcgtg atttgcgcca gctggttgcc gatggcaaat tccgcgaaga
    52921 tctatacgcg cggatcaatc tctggacctt caccctgccg gggctacgcc agcgtcagga
    52981 agatattgaa cctaacctgg attatgaagt ggagcgccac gcttcactca ccggcgacag
    53041 cgtgcgtttt aacaccgaag cgcggcgcgc ctggctggcc tttgcgacat ctccccaggc
    53101 aacatggcgc ggtaactttc gcgagctttc tgccagcgtc acgcgaatgg caacctttgc
    53161 ctctagcgga cgcattactc tggacgtggt tgaagatgag ataaaccgtc tgcgctataa
    53221 ctggcaggag agccgcccct ccgcgcttac ggcgttgctg ggcgcagagg cagaaaacat
    53281 cgatctcttc gaccgtatgc aactggaaca cgttatcgca atctgccgcc aggcaaagtc
    53341 gctttccgca gccggacgcc agctttttga cgtttcgcgc cagggcaaag ccagcgtcaa
    53401 tgacgcggat cggctacgca aatacctggc gcgttttggt ctgacgtggg aagccgtgca
    53461 ggatcagcac agctccagtt gaatatggtg gtccgtcagc acctgcatca cgcttactgg
    53521 cggcggggtg tcggtgtaga cggcatctac catgctgatg ctacccatat tgaccatcgc
    53581 gttacggcca aatttcgagt gatcgacaac cagcataacg tggcgcgagt tctcaataat
    53641 ggcgcgtttg gtgcgaactt cgtggtaatc gaactccagc agcgagccgt cgctatcgat
    53701 gccgcttatc cccagaatgc cgaaatcaag gcggaactgg gagataaaat cgagcgtcgc
    53761 ttcgccaatg atcccgccat cgcggctgcg taattcgcca ccggcgagaa taatacggaa
    53821 atcctctttt accatcagcg tgttagcaac gttaagattg ttggtgacaa tgcgcaaatt
    53881 gctgtgattg agcagcgcgt gcgccaccgc ttccggcgtg gtgccgatat cgataaacag
    53941 cgtcgagcca ttggggattt gctccgccac tttgcgggcg atgcgctctt tttcttcggt
    54001 ctgggtggct ttacgatcgt gccacggcgt gttaaccgaa ctggaaggca gcgccgcacc
    54061 gccatgatgg cgcaggatca ggttttgctc cgccagctca ttgaggtcgc ggcgaatagt
    54121 ctgcgggctg acggagaagt gctccaccag ctcctcggta ctgacataac cctgctgttt
    54181 aaccagttcg ataataccgt tgtgacgttg tgtttgtttc atttctaaat ccctggaatt
    54241 attttcgttt tcgcgcattg agcgaatcaa caaaagccat cgctaaaccc acggctaacc
    54301 cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa atcaaaccat ccggcgacaa
    54361 tccagatcag cgcaaagata attaacccac gttgcaggta aatgccactt tgcggatcgc
    54421 gttcgccacg tagccagacg tagcccatca gcgcatacac cacgccagaa agcccgccaa
    54481 accacggccc gctgaatttt tgctgcacat agccgcttaa cagggcgctg ataagcgtaa
    54541 tgacaattag cttaccgcta ccgaggcgtt tttccaccgc accgccgaga taccaccacc
    54601 agagcaggtt aaagaggata tgcatcagcg agaagtgcat taacgcgtgg gtgaagtaac
    54661 gccagaactc aaatttcagt gctggatcga atggccaagc cagccataac atcacttcct
    54721 gatcgccgag aatttgcatg gcaataaaca ccaccacgca ggcgatcatc atcacccagg
    54781 tcaccggacc tgcgcgttca cgcaaggcgg caaagaaagg ataacggcga taatgcaggc
    54841 cactgccggt atggcctgcc tgccagctcg ccgccagata acgcggatct gccgggtttt
    54901 cgagaaaacg cgccagctcc gcccgtacgc gctcggcctg ggactcatcc gccagccaga
    54961 catcgctttg gttatgttgt tgaatcgtga ggataacacc ctgcgtcgcc atgtaatcaa
    55021 caaacgcctg cgccacgcgg gggttagcaa aagaggtaat catcaacatc gttgctgtcg
    55081 cttattccac acaaaagggg acagtataaa gcgttacgcg ccgtacgcca cctctgcggg
    55141 aaactgacgt tgccaggctt caaagccgcc gtcaatgcta tagaccacat cgtagccctg
    55201 ttgcagcaga tactgcgccg cgcctttgct gctattgccg tgataacaca tcaccatcac
    55261 cggagtgtca aagtcgttat tacgcataaa agcgcccagc gtgtcgttgg ttaaatggaa
    55321 agcctgcacc gcatgtccca ttgcgaaact ctgtggatcg cgaatatcga ccagcaccgc
    55381 ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta atacattcga actgatccat
    55441 gcgtctctct ttctttacaa acaagtgggc aaatttaccg cacagtttac gtcgaagcgg
    55501 cagataaacg ccataatgtt atacatatca ctctaaaatg ttttttcaat gttacctaaa
    55561 gcgcgattct ttgctaatat gttcgataac gaacatttat gaactttaac gaaagtgaat
    55621 gagggcagca tggaaaccaa agatctgatt gtgataggtg gcggcatcaa tggtgctggt
    55681 atcgcggcag acgccgctgg acgcggttta tccgtgctga tgctggaggc gcaggatctc
    55741 gcttgcgcga cctcttccgc cagttcaaaa ctcattcacg gtggcctgcg ctaccttgag
    55801 cactatgaat tccgcctggt cagcgaggcg ctggctgaac gtgaagtgct gctgaaaatg
    55861 gccccgcata tcgccttccc gatgcgtttt cgcctgccac atcgtccgca tctgcgcccg
    55921 gcgtggatga ttcgcattgg tctgtttatg tacgatcatc tgggtaaacg caccagcctg
    55981 ccgggatcaa ctggtttgcg ttttggcgca aattcagtgt taaaaccgga aattaagcgc
    56041 ggattcgaat attccgactg ttgggtagac gatgcccgtc tggtgctcgc caacgcccag
    56101 atggtggtgc gtaaaggcgg cgaagtgctt actcgtactc gtgctacttc tgctcgtcgc
    56161 gaaaacggcc tgtggattgt ggaagcggaa gatatcgata ccggcaaaaa atatagctgg
    56221 caagcgcgcg gcctggttaa cgccaccggc ccgtgggtga aacagttctt cgacgacggg
    56281 atgcatctgc cttcgcctta tggcattcgc ctgatcaaag gcagccatat tgtggtgccg
    56341 cgcgtgcata cccaaaagca ggcctacatt ctgcaaaacg aagataaacg tattgtgttc
    56401 gtgatcccgt ggatggacga gttttccatc atcggcacca ccgatgtgga atacaaaggc
    56461 gatccgaaag cagtgaagat tgaagagagt gaaatcaatt acctgctgaa agtgtataac
    56521 gcgcacttta aaaaacagct tagccgtgac gatatcgtct ggacctactc cggtgtgcgt
    56581 ccgctgtgtg atgatgagtc cgactcgccg caggctatta cccgtgatta cacccttgat
    56641 attcatgatg aaaatggcaa agcaccgctg ctgtcggtat tcggcggtaa gctgaccacc
    56701 taccgaaaac tggcggaaca tgcgctggaa aaactaacgc cgtattatca gggtattggc
    56761 ccggcatgga cgaaagagag cgtgctaccg ggtggcgcca tcgagggcga ccgcgacgat
    56821 tatgccgctc gcttacgccg ccgttatccg ttcctgaccg aatcgctggc acgtcattac
    56881 gcgcgtactt atggcagcaa cagcgagctg ctgctcggca atgcgggagc ggtaagcgat
    56941 cttggggaag atttcggtca tgagttctac gaaacggagc tgaaatatct ggtcgaccac
    57001 gagtgggtcc gccgcgccga cgatgccctg tggcgtcgca caaaacaagg catgtggctg
    57061 aatgcggatc aacaatctcg tgtgagtcag tggctggtgg aatatacgca gcagaagttg
    57121 tcgctggcgt cgtaaattaa cgtaaggtga tcaggtcaga tttcaatctg gcctgagact
    57181 gatgacaaac acaaaattgc ctgatgcgct acgcttatca ggcctacgtg gtttatgcaa
    57241 tatgttgaat ttgcatgatt ttgtaggcct gataaggcgt tcacgccgca tcaggcatga
    57301 acaaagcgcc acgttgtcaa caatctgacc tactttttga ttcagggaat taccgcaaag
    57361 cccacgccat catacccaac gtaacaaaac ttaagcacgt tccccactca ctccacttcc
    57421 gtatctggct tctatttttg ctacgccact ggcaataata tcccaacgca aaaccaagct
    57481 gtgcacttat cggaccgaca aagtcgatcc actgttgaga ccaaaatggc gaaaaattca
    57541 gtgggagcca gacaactgtc agaaagaaac tgcgcatatt ttccagcaaa gcaatttgcg
    57601 ctggcgcgac ataaaaaatg atattccaca ggagcaacca gaaataaact ggtgcaaata
    57661 ccagccaaca atgcagtctg cttttcaatt caggtggcgt tctgcgcgca aattgccaac
    57721 caacgcccac cattgcacag aagaaaagcg catattcagc cgataaccat agttgtaatt
    57781 gcagaaggga aatacgcctg tgcatgagcg ggattgagaa cagtatttct cccagaaaac
    57841 tcagcgtgat tacacaggca gccagcgcaa ccaccgtcgg ccacaccaac aacggtacaa
    57901 taacaaagcc gagaaataac ggataaatat aaagtctgat tatattcatc attattgtcc
    57961 tgatgattcg ggggatgctt catacagcgg cacatacccc acatcctcta ccaactgttg
    58021 cccctgctga cttataaacc agtcaacaaa tttctgcgtt tccggcgtgg gattttcacg
    58081 cgtcaccata taggcatcca caatgtatgg ataagtgccg ttgcggatat tttcctcggt
    58141 aggcgcaacg ccatttatag ccaaaagttt aatctgcttg cgcattttga gcatattgtc
    58201 attcatacga gtgacgtaat agtggaaggt gtacccaatg gaagatggcg tgttttgata
    58261 gtcggcaact tcagtaatta aaccgcccat aagatcaatc acagtggatt ttttagctgg
    58321 cagcattggg gtgtcttgca taaccaaccc cttcataatc gtttggcttc cagaatcttc
    58381 agggcgctgc cagactttta tatgttcgtc actaccacct actttattcc agcggctagt
    58441 tttgccgcta aaaatatctt taacctggtg ctcagagaga gaatttaccg ggttattaat
    58501 atcaacgata aagacaaagg cttcgcgggc gaaagcggta tatttcagtt taacacccgc
    58561 ttcttctgca cgttttttct gtccttccga cggttgtgca acaaaaataa ccgtcgcgga
    58621 acctttaatc agactatcgt aggcttcagc tgttcgggtt tgctgcaaac catacgcctg
    58681 ccaggccagc gaggttcgat ccttatcttt tggctcgggt actaatttat aatacgcgga
    58741 ggcatacagc gggtataagg ctgtagcgcc gtacactctc ggccatcttg accaatcatc
    58801 gctgtaacgt aatgcaggtt gcccccgcag agacgtcagt ccatggctct cttcttccct
    58861 gacgctgtta tagtaccctt cccggtcgac acatcccgcc agtaacgccg tgcctaatag
    58921 taaaacaccg gtgaatttat tcatctcgct gattcctttc tctcgagcgt tattccattg
    58981 ttagatacag cggcacatat cccacatctt ctaccagact ctgcccctgc ggcgttaaaa
    59041 accattcgac cagtttttgt gtttctgacg ttgtattttc tctcgtcacc ataaatgcat
    59101 cgacgatgta cgcatatttg ccgttgcgaa tgttttccgc cgtcggtgta ataccgttaa
    59161 tcgctagcaa tcttatattt ttatcagcat tcatttgcgt cgcgtaatag cggaaggtat
    59221 agcctattgc gttgtttgta ttacggtatt cggcaacgac tttaatcatt ccctccatca
    59281 cgcttgccac ttccgtttcc tgcggcgaga tcatgcggac ttttttcatg acctgtgatt
    59341 gcatcactgt ctggctgcca gagtcttccg ggcgctgcca ggtctggatc tcctgatcgt
    59401 taccgccaac agtacgccaa ttggtaattg caccactgaa aatgtcacgc acctgttgtt
    59461 cagtcaggga attaaccgga ttatccgcat tgacgatgaa aacaaatgct tcacgggcaa
    59521 atggcgtgta tagcaaagtg acgcccgatt cctcagcgcg ttttttctgc ccgccggagg
    59581 gttgcgccac aaaaataata tcggcatcac ctttaacaat ccggttatat gcatcggggg
    59641 tacgagagtt ctccagatat tcccaaacgt gaaaatcctc tggtattaca cttaatgcat
    59701 aaaatgcaga agcataaatt gggtacgcag ccgtggcgcc atcgattcgc ggccagtttt
    59761 gcctaaactg aatttgcggt ttgccacgca gcggcgtcag tcgattattg agtttatcgg
    59821 gtcgccaggc cctgatatca atattttcgc gtacaccgac accaggattt tgctgcttat
    59881 ataaatgggc ctgatacccg gcgacaatac caagtaagag aagtagaaac aggagtaagt
    59941 tacggcaacg tagtccggac gtgccggata aaggatatct ggtcggccag gcgtatccca
    60001 gcgaaaaact gatatgcgcc ccaacgggaa taatgaccat ccaccagtac tcaccaccca
    60061 ctaatgagac taaaaaaacg cccaaccagg gaagcataag gtttgccaac caaccagctc
    60121 ccatctggcg aaattgataa tctgccagat gtagcgtgat tatccagcca accagggtta
    60181 atagcgcggg taagagaaaa aagcattgcc gccagacggc tatttctctg ttttctggcg
    60241 cgctatcacc ggcccacacg cgaccagaga aaaatattat tatcgccccc agcgtcagca
    60301 taaagacgtt gagtagaaga taaattgttg gcgtgataag gatattacaa acttcaataa
    60361 taccggtaag cccgacaccc agcatggcaa caaccgctgc caaaaattgc gccagtatgg
    60421 ggatgccgaa aaaagtcatt atcagcgagg tcaaaatcca ttttctattt ggcatcattc
    60481 tgtccattct tcctgaatga cggaattata ctccccgaga accctcgccc cctctggaca
    60541 cttttccgaa ataaaggcga aaaaaacggg accttgtggc cccgttctgt tttttagcga
    60601 acttacaatc tcaccggatc gatatgccag atatgatcag cgtactcttt gatagtacgg
    60661 tcagaagaga agtagcccat attggcaatg ttcagcatcg cttttgcggt ccactcttcc
    60721 tgaagctcgt agagttcatc gactttatcc tgacaatcga catagctgcg ataatccgcc
    60781 agtacctggt agtgatcgcc gaagttgatc agcgaatcaa ccagatcgcg atagcgaccc
    60841 ggatcttccg gactgaatac accgctgccg atttgcgtca gcacctgatg cagctcctca
    60901 tctttctcgt agtattcacg cggtttgtag ccctgacgac gcagttcttc cacttcttcc
    60961 gctgtgttac caaaaataaa gatattgtca gcaccgacat gatccagcat ctcgacattc
    61021 gcaccgtcca acgtaccgat agtcagcgca ccgttaagcg caaacttcat gttactagtg
    61081 ccggaagctt ccgtccctgc cagcgaaatc tgttcagaca gatctgccgc cggaatgatc
    61141 aactgcgcca ggctaacgct gtagttcggg atgaacacga ctttcagctt atcgccaatc
    61201 tgcggatcgt tgttgatcac tttcgctacg tcattgatca aatggataat atgcttcgcc
    61261 atgtaatagg ccgaagccgc cttaccgcca aaaatattca cgcgcggtac ccacttcgca
    61321 tccaggtcgg ccttgatgcg gttatagcgg gtaatcacat gcaacacatt catcaattga
    61381 cgtttgtatt cgtgaatacg tttgatttgc acatcgaaca acgcctttgg attcaccacc
    61441 acattcagct gctgggcgat atactccgcc agacgctttt tattctccag cttcgcctga
    61501 tgcacagcgt gattaaccat tgggaaatca cagtgttgtt gcagctcatt aagcaggcta
    61561 aggtcggtgc gccagttacg gcccaggtgt tcgtccagca cggctgaaag cgatgggttc
    61621 gctaccgcca gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa acgacccggg
    61681 aagattttcg caaagtcggc aaacaacgat tgcaccatca gattagagtg cagttccgat
    61741 acaccgttaa ctttgtggct cacaacaacc gccagccagg ccatacgcac acgacgaccg
    61801 ttggattcat caatgatcga cgcccgtccc agcagatcgg tatcgttcgg atactgttcc
    61861 tgcaaggttt tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg acgcggcaga
    61921 attttaccca gcatatcaac cggccaggtt tccagcgcct cgctcatcag cgtgtggtta
    61981 gtgtaggaga agacctgaca acacacctca aacgcgtcgt cccagctaaa ttggtgctca
    62041 tcgatcagca gacgcatcat ctcaggaatc gacagtaccg gatgggtatc attgagatga
    62101 atcgcgattt tatccgccag gttatcgtag gttttatgca actgataatg gcggcttaaa
    62161 atgtcctgaa tggtcgagga aaccaggaag tattcctgac gcaggcgcag ctcacgcccg
    62221 gagtaggtgg agtcatccgg atacagtacg cgagatacgt tctcggagtg gtttttatct
    62281 tccactgccg cgaagtagtc accctggttg aatttaccga ggttaatttc gctactggct
    62341 tgcgcactcc acaaacgcag cgtgttggtc gcgtcggtgt cgtaaccagg gattatctga
    62401 tcgtaagcga ctcccagaat ctcttcggtt tcaatccagc gcgttttttt accttcctgc
    62461 tgaatgcgac caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa ttcccacggg
    62521 ttaccatatt ccagccagta gtcaggcgac tctttctggc taccgttaac gatgttctgc
    62581 ttgaacatac cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc taacgtcgcc
    62641 agagaatcga ggaagcaagc cgccagacgt cccaggccac cgttaccgag gcccgggtca
    62701 ttttcttcat caatcagctc ttcgagattt aaccccatcg cttccagtgc gccctgtaca
    62761 tcttcgtaaa ttcctagcga caacatggcg ttggagagcg tacggccaat caaaaactcc
    62821 atcgacaggt agtaaacctg acgagtttct tgcgacaact gggcacggtt tgaacgtaac
    62881 cagcgctcca cgagacgatc gcgcacagca aataacgttg cgttcagcca ttcatgttta
    62941 ttggcgacga ccgggtcctt tccaatcgta aacatcagct tgtaagcgat agagtgctta
    63001 agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat ataggcgttt
    63061 cctgaaaact atttcaagcg atagtaaagc tcacggtacg acttcgccgc gacctgccag
    63121 ctaaaatcca ttgccatagc ctgacgttgc acaaaccgcc acagagaagg acgggaccac
    63181 agtacaaaag cacgtcgaat agcccgtaac agcgaccagg cattactatc ttcaaagaca
    63241 aacccactgg cgacgccatc tgcaaggttc tcaagagaac agtcagaaac cgtatcagca
    63301 agcccaccgg tgcgccgcac taacggcagc gtaccgtact tcaatccata aagttgcgtt
    63361 aagccgcacg gttcaaaacg gctgggcacc agaatgacgt ccgcaccgcc cataatgcga
    63421 tgcgaaaatg cttcgtgata gccaatctga acgcccacct ggccggggta ttccgctgcc
    63481 gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc cagctgcccg
    63541 ccctgctcca gaagacccgg taaggcctcc agcaccaggt cgaggccttt ctggctggtc
    63601 agacggctca ccactgcaaa aagcggcact ttatcgtcaa ccttaagccc cattgcgatt
    63661 tgtaactggc gcttattttc cgctttatct tccaacgtat cgcgggtgta acgcgaggcc
    63721 aatagtaagt ccgtctctgg actccagatt ttctcgtcca cgccgttcag tacgccggaa
    63781 agacgccctt cacgatgacg ctgttgcaac aaaccttcca taccgtaggc aaactgcggt
    63841 tcggtgatct cgcgagcgta ggttggactg accgccgtaa tgtgatcggc atagtacaga
    63901 ccggccttca ggaaagagat ttgtccgttg aattccagcc catgaatatt aaagaatgac
    63961 catggcaatt ggatgtcatt catgtgatgt gcataaaaca tgccttgata ggccaggttg
    64021 tgcacagtaa acaccgactt cgccggacgc ccgcgcgccg ccagatacgc aggcgcaagg
    64081 cctgcatgcc agtcgtgcgc atgcaccaca tcaggacgcc agaatgggtc aagcccgctg
    64141 gccatttctg ccccaaccca ccccagcagc gcaaaacgca atacgttgtc ggtataggca
    64201 aataagttgg tatcgtgata cgggcttccc ggacgatcat agagatgcgg cgcgtcaatc
    64261 aggtatatgc caaccccgtt gtaatgaccg aacaacagcg tgatatgccc ggcgaaggta
    64321 tcacgacggg atactacctg cgcatcggtc acgccacggc gaatatcggg aaatgcaggc
    64381 aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc cccaataaca
    64441 tcagccagac cgccggtttt aagcagcggg aacatctctg aacatacatg taaaacctgc
    64501 attatcgctc ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc agcacgatgc
    64561 cttcttctga acgatagaaa cgacgtgcat cttcctctgc gttttcacca atcaccatgc
    64621 cttccggaat aacacaagca cgatcgatga cgcagcggcg cagacggcac gagcgaccta
    64681 cccatacttc cggtaacaat acggcggaat caatgttgca gaatgaattc acgcgaacgc
    64741 gcgagaacag aacggactgc accaccaccg aaccggagat cacacaaccg ccggaaacca
    64801 gtgagttaag ggtcatcccg tggctaccgg agcgatcctg cacgaatttc gctggcggta
    64861 atgattcatt gtaggtgcga attggccaat tgcgatcgta catatccagt tccggcacca
    64921 cagaggccag atcgaggttc gctttccagt aagcttccag cgtacccaca tcgcgccagt
    64981 acggctcggc atccggatcg gattgtacgc aagagagcgg gaacgggtgc gcataggcca
    65041 gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg gagttctcat
    65101 cgcgatcgtc ttcttccagc agttcataca gatagtcggc gtcaaagacg tagataccca
    65161 tactcgccag agatttgctc ggatcgttcg gcattgacgg cgggttagca ggtttttcaa
    65221 cgaattcgat aattttatcg ttctcatcaa ccgccataac gccaaatgcg gaggcttctt
    65281 caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg tgatcgataa
    65341 gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc acgtattccg
    65401 ctttataacg gcgaataata tcgaggtttt gggtgaccgc atctgcggtg ccgcgatacc
    65461 agttttcccc tttcattctc tgctgtgctg gcagcagatc gacaaactcg ttcatttctt
    65521 cattgaagaa tgaccagccg cgctgaatgt gctgcaccag agtgtgggac tggtactggg
    65581 tgatcacgcc catacgacgg atcccggagt tgatgcagtt agacagcgca aagtcgataa
    65641 tgcggaactt accgccgaag tgtacggccg gttttgctcg cttattggtt aaatccttca
    65701 ggcgggtacc acgtcctccc gccagtatca gggcaacaga tttcaatggc agctggcgcg
    65761 ccaacattaa gtgatcgttc ttctctaaac taaccatgac taactccttt tttatcatct
    65821 ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca gccgtaatca ctgggttatc
    65881 ctctccagcg aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa tctctgttac
    65941 ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag agcagaattt gcagctgttt
    66001 cggcccgttt tgccactcat ccgtgcttaa aggttgagca tatcgattta gccaacggac
    66061 attgccgtcg ccttcttccc accagcgatt ctccaccaaa gcgggaatac gcttgcgcag
    66121 atggattaac gcggcggtaa atgcggttaa accactgctt gcctgcgacc agtccaacca
    66181 ggttaattgg ttatcctgac agtaggcatt gttattgcca tgctggctgt gaccatgttc
    66241 gtcaccggcc agtaacatcg gcgtaccctg ggagagcaac aacgttgtta acagggcgtg
    66301 aatgctgtcg cgccgccgtt caaccaggtc aagggtaccg cctacccctt ctttaccatg
    66361 attgttactg taattgttgt tggtcccgtc gcgattttct tctccgtttg cttcattgtg
    66421 tttatggttg aaacaaacgc agtcgcgaag cgtaaaaccg tcatgcgcgg tgacgagatt
    66481 aatcgcggca ctcggcagac gaccattacg tttaaaaaca tcgctggagg cagcaaaacg
    66541 cccggcaaac gcccccagag gcaaatcata atgtagccag aaacgacggg cagcatcgcg
    66601 gaaatgatcg ttccactcgg caaacagcgg cgggaaattt cccacctgat aaccaccagg
    66661 agcgatatcc cacggttcag caattaactt cacctgcgag agcaccgggc agttctggat
    66721 agcggtaaac aacggcgcat cctgacggaa ctctggcgta cggcccatga ctgccgccag
    66781 atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa taacgcaggc aggcgctggc
    66841 ataatccacc accgccgggt gactcaaatt gagcgtgttg ccacaaccgg tccagttgtg
    66901 ataatcgccg tcttctctta tccaataata gctacggttg tcgatcccac gcagcgagaa
    66961 taacgggccg tcgaggtcca gttccgcact atggttgagc acgatatcaa gaatgacttc
    67021 gatacccgct ttatgcagtg ctttgattgc atcgcgaaac tcatgcagcg ccgtttctgg
    67081 cgagcaggca tacgccggat gcagcgcaaa catcgccacc gggttgtaac cccagtagtt
    67141 acttagcccc atgcgttgca gacgtggttc actggcaaac tgcgccactg gcagcagttc
    67201 cagcgcggta atgcccaatt gtttcaaata gttgatcatc accggatgcc cgagggcttt
    67261 ataagtgcca cggatctcga ccgggatctc cgggtgcagg tacgttaatc ctttaacatg
    67321 ggcttcataa atgatggtgc tgccccacgg cgtgcgcggc ggggcatcat cttcccagtc
    67381 atagtgatca accaccacta cgcatttcgg cgcaatggcg gcgttgtcgc gatagtcagg
    67441 ttcattatga ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa tttgccgcgc
    67501 gcaaggatca atcaacaact tcgccgggtt aaagcgatgc ccctcggcgg gttgccaggg
    67561 gccatgaacg cgataaccat aacgcaaacc cgggcgcgca tccggcagat aaccgtgcca
    67621 aatgtcgcca ctgtgccctg gcaagtcata gcgatgttcc tggccattgg cgtcaaagac
    67681 acacagttct acccgctcgg catgagcgga gaaaagtgtg aagttgacgc cctgaccgtc
    67741 gtaatgcgcg ccgaggggag cgggtttgcc aatggcgagt tgtgtcattc tgcctcccga
    67801 accagccaga tagtggccag cggtggtagc gtcaggctta gtgaatgctg acgaccgtgg
    67861 ctggcaatct catcgctgtg taccgtgccg ccattacctg cattactgcc gtgatagtgc
    67921 atggaatcgg tattgaggat ctcacgccat ttgcctggct gattaatgcc gaagcgataa
    67981 tcatgacgcg gcaccggcgt aaagttactg gcaacgatga tttcgttacc ctctttatcg
    68041 cgacgcacaa agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg
    68101 tacgggtcaa aatccagttc atgcatcgct ttatggtggc ggtaggtgag gttcagatcg
    68161 cgcaccagac gctggacacc gtggtgccag ttatcgccgc cttccaacag atgccagtcg
    68221 aggctggcgt catggttcca ctcgcggccc tgggcaaatt cgttccccat gaacagcagt
    68281 ttcttgcccg gaaatgccca catccagcca tagtaagcgc gcaggttagc gaatttctgc
    68341 catgcgtcgc ccggcatgcg gtcgagaatc gattttttac cgtggaccac ttcatcatgc
    68401 gacaacggca ggacgaagtt ttcagtgtag ttgtagagca tcccgaaggt cagtttatcg
    68461 tgatgatact gacgataaat cgggtcgagc ttcatgtagt ccagggtgtc atgcatccag
    68521 ccgaggttcc acttgtacca gaagcccaga ccgcccatat cctgcggacg agaaacgcca
    68581 gggaaatcgg tagactcctc agccattgtc accgcaccgg aaacctgctc accaagaata
    68641 cggttggtat tacgcaagaa ttcaatcgct tcaagattct cgcgcccgcc aaattcgttc
    68701 gggatccact ccccctcttt acggctgtag tcacgataaa tcatcgacgc caccgcatcg
    68761 acgcgcagcg catcaatacc aaaacgttca atccagtaaa gcgcgttacc gacgaggaag
    68821 ttactgactt cacggcgacc atagttgtaa atcagcgtgt tccagtcctg atgatagcct
    68881 tcgcgcggat cgctgtgttc atacaagttc gtaccatcaa attcggcaag cgcaaagtca
    68941 tcagtcggga agtggcctgg cacccagtcg agaatcacgt tcagaccagc tgcgtgtgca
    69001 gcatcaatga aataacggaa gtcgtcgcga gtaccaaaac ggcgggttgg tgcatacagg
    69061 ccggttggct gataacccca actgccatcg aagggatgct cgttaagggg cagtagttcg
    69121 aggtgggtaa agcccatcca tttagcataa ggcaccagtt gatcggccag ctcgcggtag
    69181 ctcaaccaga aattgttgtc ggtgtggcga cgccaggagc ccaggtgaac ttcataaata
    69241 gagattggcg catcaaactg attcgctttt ttgcgctctt cagtctgtac aaccttttcc
    69301 ggcagcccgc aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa ggcataaggg
    69361 tcggacttca gacgcaagtt gccattggca tcaatcatct cgtatttata gagctgaccg
    69421 ttatgcgccc cagggataaa cagttcccag atgccgctct ctttacgcag gcgcatcggg
    69481 tgacggcgac cgtcccagta gttgaattgc ccaaccaccg agacccgacg ggcgtttgga
    69541 gcccagacag agaaacgcgt acctgtgacg ccatccatag tatctgcatg cgcgcctaaa
    69601 gtttcatacg ggcgcaggtg agtaccttca gataatagcc aggcatccat ttcctggatt
    69661 agcggaccaa aacggtaagg atcatcaatc aggttttgct gcccatgcca gacaacagcc
    69721 aactgatagc ggaaaaaatt cttacgtcgc ggaatgacgc cactaaagaa tccccgtgag
    69781 tcgagacact ccagttttgc gagtttgcgc ccggttttcg gttcaatcac ccacacatcg
    69841 gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg tggttttatg cattcccagt
    69901 acggaaaaag gatccgcaaa atggcctgca attagcgcgt taatcacgtc tctatcgata
    69961 cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc catccaattg gatttttgac
    70021 ttctggttgt gacatttttt tgacctgaac ggcgcagcac tctgtgcatc ctctctgcgt
    70081 cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc agccaccctt aaagaatagc
    70141 caatgctcta tttaactccc ggtaaatcat gaaacatctg cgcttactcc tgtattacgc
    70201 actaacaggg gcggcatcgc gccccagatt taatgaataa agattacgcc agttgacgaa
    70261 gcatccgacg cagcggctcc gcggcccccc acagcagctg gtcgcccacg gtaaaggctg
    70321 acaggaactc tggtcccata ttcagcttac gcaggcggcc taccggcgtg gtcagcgtgc
    70381 cggtaacggc agctggggtt agctcacgca tagtgatttc ccgatcgttc ggaacgactt
    70441 tcgcccacgg attgtgcgca gccagcagtt cttccacggt cggaatcgac acatcttttt
    70501 tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc acacataaac
    70561 catctaccgg aattacggaa gatgtgttga ggatcttgtt ggtttccgcc tgccctttcc
    70621 actcttcgcg gctctgaccg ttatcgagct gtttgtcgat ccacggaatc aggctacccg
    70681 ccagcggcac gccaaagtta tccaccggca gctcaccgct acgggttaag gttgtgactt
    70741 tgcgttcgat atcgagaata gcagaggacg gggtcgcgag ttcatctgct acatggccat
    70801 acagatggcc catctgggtt aataactcac gcatatgccg cgcaccaccg ccggaagcgg
    70861 cctggtaggt tgcaacggac acccaatcaa caagatcatt ggcgaataaa ccacccaacg
    70921 acatcaacat caggcttacg gtacagttac cgccaacaaa agtcctgatg ccattattta
    70981 atccgtcggt aatgacgtcc tgattgacgg ggtcaagaat gatgatggcg tcatctttca
    71041 tgcgcagaga cgatgctgcg tcaatccagt aaccctgcca tccgctttca cgaagctttg
    71101 gatagatttc gttggtataa tcgccgccct gacaggtcac aatgatatcg agggccttta
    71161 gcgcctccag atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa gacggcgcag
    71221 cctgaccaag ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag tcgcgctctt
    71281 caaccatgcg ttgcatgaga acggagccga ccataccgcg ccagccgata aaaccaacat
    71341 ttttcataag cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt
    71401 ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag
    71461 cgaagccatc agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat
    71521 tatcagggaa tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt
    71581 atggatccgc tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa
    71641 agacggcggg caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc
    71701 ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc gagcagaaac cgtctccatt
    71761 tctggcggca tcattctgtt tctaattgcc attaagatga ttttccccag cgcttcagga
    71821 aatagcagcg ggcttccggc aggtgaagag ccatttatcg tgccgctggc aattccgctg
    71881 gtcgccgggc caactattct cgccacgctg atgttgttgt ctcatcagta cccgaatcag
    71941 atgggacatc tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta
    72001 cagtcttcgc tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg
    72061 atgggattga ttctggtgat gatggcaacc cagatgttcc tcgacggcat tcgaatgtgg
    72121 atgaaggggt aaggttcaaa caggtgctgc acacaaccag acgcggcacc tgttttatta
    72181 cctgaactga taatgacctt cgcccaatcg gataaccctt ccctcttctt ctgcgcggtt
    72241 cagccacaca ctaatttgtt cgatggttaa accagtacaa gacgccagcc tttcaatact
    72301 tatcgattcc tttgcaagga tcgccagttc cgccacaaag agttgataaa aatcgacagg
    72361 cgtatacgcg ggcattactg cgtaatttgc gtcatcttca tgcagcgaaa acagttctgg
    72421 ttgagcgtta cggagtgctc tgtcatacat cagcccagga acgtctggca gtctgatcgt
    72481 cacattctca gcctgttcag cactccatga cgccccattt tctactaatc gtgaattggc
    72541 tgattgcata tcctgatttg gtttaacctg cacaggcagc cactgatgtt ttaatgcctc
    72601 catcgccccg gttatcgttc caccggtcat tcccgcacgt acaaccattg cgctttcagc
    72661 aaggcaataa atatatttat ttcgcgccat cgcattgccg acggtgaaac gtacctctgg
    72721 gtaaaacggc gaaatcaaca ccaggttgcc tgctataagc ccttcacgcc atttcactaa
    72781 cgtactcgtt tttaacaagc tatcagctaa tacgccaacg gcagttcccc cggcctccag
    72841 tgccgacgcc attgcacatt catcaatacc tcgcgcacca ccagagataa cgcaaatccc
    72901 ctgttgggcc agtttcgcgg ccagttgttg ggtatagcgc aaatcgtcag tcggagcatc
    72961 tcgcgagcca acaatcgcca taccttccgc ttgcagtaat gctttattcc cgcagccaaa
    73021 taaaacggga ggtgcatccg ttcgcaatcg gtttttcaag cgaacgggat aatcagcatc
    73081 tccgcgggtt aagatccata aaccggcgcg ttgccactta tctaccgcca acgccagact
    73141 atgaccacgc gccagcaagc caagaatacg ctcctgagaa atacgcggat cgctccagcc
    73201 tgtaagtttc tcttgcggtt gcggcaccag aagctcggcg ggactgatac gttggtgctt
    73261 cagccataat gcaaacttcc cccattcact attactaaga ggtttatact cactcgccgc
    73321 cgcgcgagaa aaatcgctgg ttagcaggag agttgcttgt gcattggctg aaagattcat
    73381 caatttttta ccgaggtaga agcaagggca agaggataaa ccgtcgggca acctgcctgg
    73441 cgtaacagtg ctgttagaac tgtcagcgtc catgcagaat ccacgatatc gtcaaccagc
    73501 aacgccggac caggcatcaa aggaggggta atcacaaacg ccccgtcgag attttgacac
    73561 tggtggaaac ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca
    73621 tcaataaaag gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg
    73681 aggtgctttc aaaatcagga accaggtcga ggtgctttaa tgaaggaacg cagcataccc
    73741 aggttggttc aggatgcgga ttccagcgtt ggcgaaccat ttccgcacat gcctccacca
    73801 gttcatcact gaatcgcccc gtttttttct cctgtgctac ctgctttccc cacccggaat
    73861 atccccaacg agaaaggatt cgccccgtag atccttgtaa actcgcaggc aagttccctt
    73921 taaatccgta ttgcgtaaaa gccccagctg ccacctgttt attgagattt aatgacaggt
    73981 cagcgtgttt aataaataag tttgccgcat ggagtaagcc gctatcaatg tcgggcgaca
    74041 acaacggatg ctgaagacaa ctgctacatt taccacaatg ttcggcacct ggctcatcga
    74101 gtgcgcggcg cagaaattgc attttgcact ccttggtcgt gatatagctt tcaacgtctg
    74161 ccagctcact ttttctctga ttcatcaaat ggttaattcg ttcatggtca ggagaaaaac
    74221 tgacaattgt tctgcgccat aatttctcgg tatacaccac aggcgatgga ttttcagcta
    74281 ccaataattt cagtgctttt tctatttgcc cataacggag attcgtccgt tgttcaatgc
    74341 ctcgtaaggt aagaccgtca ttcacgctaa gtacgttgag tatttcatga atttgcgcct
    74401 ccgcaggaaa ggcgctttca cggaagaatt tatgaatagc acggtcttca ctgccgcaaa
    74461 gcagtatgcc aacagcagaa tcgataccgc gaccggcacg ccccacctgt tagtagtaac
    74521 caactatcga acctggcatc tggtaatgaa taacaaaccc caaatccggt ttgtcaaagc
    74581 ccattcccag cgccgttgtc gcaaccagca ctttaatttt atttgccagc aacgcctgct
    74641 ccagatattc cctggcagta ttagaatctt ccgcgccttc acaagtcacg ccgctgtagt
    74701 aagcaaatgc actgatacca tttttcctta accactcggc gacaagttcg gcatcgcgag
    74761 tagtcagggt ataaacaatt cccgatttgg aaaactgagg gataaccgtt gctaaccatg
    74821 ccagacggga tgagtgttca cctaagacta aggcatcaag cgccaaactt tcgcgagcca
    74881 gcgttccacg ctgaatcaca atgtcaccca attgctgacg gatatcctca acgacacggt
    74941 tattcgctgt cgcggttgta cccagaacag gggtattcgc aggtagttgc gcaaaatatc
    75001 taatatacgt cggtaatccg ggcgaaaatc gtggccccaa tcagagatgc agtgcgcctc
    75061 gtcgaccaca agcaagccaa tatgatcggc tataggatat aaaaccgttt cgataaagtc
    75121 ctggtttgcc aaacgttccg gggaaaccag caagcaatcg acctcacctt gcagcaactt
    75181 atcgctaatg cgctgccatt cttctctgtt tgtagaattc aacgtttcag ccgtaatacc
    75241 taaccgttca gcggcggcaa cctggttacg catcagggca agcaaaggag agatgataat
    75301 tgtcgggcca gcgccgcgat cgcggaagat tttactggcg atgaagtaca cagcactttt
    75361 cccccagcca gtgcgttgga caaccagcag ttttcgccgt tggttcacca ccgcgtctat
    75421 ggcttcccat tgcccttccc tgaaagtagc ggtggtattt cctaacatcc gctgtaaaag
    75481 caattcagct gcgtgctttt ccatctttat ctcctgcgat ctttaggcag tgattagaac
    75541 ggattatttg caggtaaatt tgcgagagag atcaaaaaga tgaagagagg agaaaagaga
    75601 aatgaattac tgcgagcaag ctcgcagtaa tttgtcgcag taattaactc aacagctgga
    75661 acgcaatcat cccaacgatg gcaccgacag tgccgaggat ggtttccatc atggtccagg
    75721 ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc gaacaaccag aaaccagcgt
    75781 cgttaacgtg gctgacaaca atcgaaccac cggcgataca aatcgacagc gccgccattt
    75841 gcgcaccgga gtagttcagt tgttcaataa ccggcatcac cagtcccacc gccgttaaac
    75901 aggcaacggt tgcagaaccc tgaatgatgc gcactgcagc ggccagcacg aagcaggtga
    75961 tggcaatcgg caggcccatg ccggttaacg cttcgcccag tgccggacct acgccagagt
    76021 caaccagcac ctgtttgaac acgccgcccg caccaatcac cagcagaatg atccccgccg
    76081 gttgcagcgc gtgaccgcaa atctccatca ctttgtcttt tggcatgccc tgacgcattg
    76141 ccaggccata aatcgctacc agacaagcaa ccagaatcgc ggtaaacgga tgaccaataa
    76201 actcgaacca ttcgtaagcg gtagagcctt ccggcacaaa acgcgcggca atggttttca
    76261 gccctaccag caccagcggc agcaggatca gcgacaggct gaatccgaag gatggcattt
    76321 tgccttcgcc gagatgcggt tcgctgatgt cgtcaggaat atgcagctca acgtaacggc
    76381 tgatgaagtt accccacagc ggcccggcaa taatcattcc cggaattgcc gcacacaggc
    76441 caatcaggat catccagcca aaatcggcgt tcatctgcga tgccagcagc attggcgctg
    76501 gccctggcac caggaacgcc gccgcggccg ccacgcctgc gaataatggg attaccagct
    76561 tcaccaggtt cgtaccggtg tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa
    76621 tcgccacttc aaagaacagc ggtagcgcac agaccagccc cgcaaggccg atggcataat
    76681 gcgcgcggct gtgaccgaag gatttaagca ttttgacggc aatctgatcg actgcgccgg
    76741 tttcatgtaa gatcttgcca aacatagctc ccagggcgac aaccaccgcc aggaagccga
    76801 gggtgcctcc catccctttt tccatcgtcg ctgcgatttt atcgagcggc ataccggaaa
    76861 aaaggccagc ccccatggac accaccatta aagccaggaa agcgtgcata cgcgccttca
    76921 tgactaaaaa cagcagcagt aaaacagacc ctactgctgt taaaacaagc gttaatgtag
    76981 tcactactta tttgcctttt ttaataacct caatggtgct tgccacaaca ccttccagcg
    77041 gttgatcgat atccaccacc agtacatcgg tttcgtccgc acccggctcc tgcagcgttt
    77101 caaactgcgt caccaacatt tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc
    77161 tttcaatcac atcaaaatcg cctttcaggt agatgaaaga gagattcgga ttaccttcac
    77221 gcagcaagtc acgatagtgt tttttcaatg cagaacagac gatcagcgac actttattag
    77281 tgcgctgcat agcaaacgcg gcgtcgttca gcgcctgcaa ccacggtttg cgatcgtcgt
    77341 cattcagcgg ttcgccagac gccatttttt cgatattgcg ccgtggatgg aggaaatcgc
    77401 catcaagaaa cgcggcatga agttgatgcg ccacttcact tgcgaccgca gatttgccgc
    77461 tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg
    77521 tgcccccaca atacaagaat taaaaatgtt acgggtaact gttatcggta acattgtcca
    77581 gccggacaat atcagaagca atatccatcc gtgccttaag tgtataagtg tgagctactt
    77641 caaatttgtg ggcttaaata gatccgcccg gtgacaaggt gaaacctaaa tctaacattt
    77701 tcggtgtcac agattcgcca cgaatacgcg ccagcaggcg ttcagcgcca atactgccca
    77761 tccgctcacg cggcgtcagc acgctcgcaa gtcgtggctc catcacctga ccaatgtcat
    77821 gaccgtggaa accggcaatc gccatatcgt caggaacttt taaccccaga cgctgacatt
    77881 caaacgccgc gccgaccgcc agatcatcat tagtacagaa cacgccatcc agctgcggat
    77941 attcccgccg tgcctggcga atcagttcaa taccggaaga gtaagaagaa gattgctcaa
    78001 ccatcacgct atatggcacc aggcctgcat ccagcatcgc ctgttcgtat cccttctgtt
    78061 tgatgatagt acgttcgtcg agacgtgcgc cgagataggc aatgtggcga tgcccgcgag
    78121 caataatggc tgtggtcatc tggcgtgccg cttcaaagtt atcaaaaccg acggcgatat
    78181 cgagacacgg cgactggctg tccatcaact ccaccaccgg gatccccgcc acttcaatca
    78241 tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat cagtccgtcg atattccatg
    78301 agagcattga ttcgagacgt tcttgctcca tttccggttt ataaccgtag tgggccagca
    78361 tggtctgata accgtgcgcg tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa
    78421 cctggttggt gagagaaggt aacaggacgc caatcgcccg gctggtggcg ttagagagga
    78481 tatcgggcgc acgattggga atatagccca gttcatcaag agccgcggca atcttgccgc
    78541 gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg gctgaccgtc attttggtca
    78601 cgcctacacg gtcagccaca tcctgaagta cgggtctttt ctttttcatc gtcctgaagg
    78661 tacaaaagag atagattctc ttagtttaac acggacgcat cagaaccttc ccggggaaaa
    78721 cagtgcttgt ttacaattat tagatataca ctaaatcaat tgcatcgcat tgtgctaacg
    78781 acgctgcaaa accccggaag cgtacatcag cacgcgaccg gagcgagcga ggaagcctgc
    78841 aaaaaggcag gcttcaaaga tgccgttgag ctttaaaccg gcggcagatc gaacagtaac
    78901 acttcgctgt cgctatccgc atggatggag attgcctgct catcccagat tgccagacca
    78961 tcgctgatcg aggctttcac gccgttaatg gtgacattgc ctttcaccac ctggatccag
    79021 acgcggcgtt cagcggcaat ctgatgcacc gactgctcat ctttcagcaa cgcccagcgg
    79081 tacagttcca tatcctgatg cactttcaat gaaccatcac gcgcatctgg cgaaagcacc
    79141 agttgtttgc cctgcactgc atcgaagcga cgctgttcat aacgcggcgt aatgccgttt
    79201 tcttccggca tgatccagat ctgatacagg tgcagacgct cggtgctgct tgggttgtac
    79261 tctgagtgac gaatacccgt acccgcactc ataatctgga actcgcccgc cggaacctgc
    79321 tctttattgc ccatgctgtc ctggtgctca acagtacctt ccagcacgta ggtcaaaatt
    79381 tccatatctt tatgcgggtg agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta
    79441 atcacgcgca gcgcggagaa gcccataaag ttcggatcgt aatagttggc aaaagagaaa
    79501 gtatgccagg agtccagcca gccatgattt gcatgaccac gttcatttgc tttgcgtaag
    79561 tagatcattt gtattcgccc cctgaatgat ttcgatgggc ttagtgtgga cccgatctgc
    79621 ctgggatgat agagggtgaa aattgacccc tctgttcaaa aaaattgaac tattcgaggg
    79681 gccatcaaac ttacttcgcg agagtcactg tggagggaga ggcttgctcg aatccacgtt
    79741 caagtatttc cagattcgta agaacttcag attccttgac gtaatttggc gcaccgtggg
    79801 tgatggtttg atacaacgca tcataaacgc gcccgtaatc gcccatctcc ggcttcatct
    79861 cttctctgac cgtcacgccc tcgtcattga catactccag cacaccgacc gaatcatccg
    79921 ctgcgaatcc cggttcgccc ggcataatat tagccttcag gctggtttcc tgctggtcga
    79981 taccatattt aataaacgaa cctttcttac cgtgaacgat aaatttcgga taatcgattt
    80041 tcaccagatg gctggttttg acgatggctt ttagatcgcc ataaaacagt tgcgcttcga
    80101 aagtatcgtc aggattggct ttattgcgca ggctgcggat gtcataagcg acgtgatccg
    80161 ggcgaccgaa cagagaaata atctggtcca tcgtatgcac accaaggcca tagaacgcgc
    80221 catcctgcgg cagcccaggt ttggtttctg ccaccgggcg gtaatagtca aaatggcttt
    80281 ccacttcgac aatctctccc agcttgccgc tttcaatcgc ttttttcgcc gtcaggaagc
    80341 aggaatcaaa gcgacgattc tgatatggcg tgacggtcag ccctttgctt ttcgccagtg
    80401 caaacagctc tttcgcctgc gcaagtgtcg gagtgaacgg tttttcgacc agcacatttt
    80461 tcccggcttc catcgcgcgc ttcgcgtact cgaagtggct gtccgcatgg gtgcagacaa
    80521 caaccagctt aacatcggga tcgtttagca cttcgttgag atcgctggtg aaatggatat
    80581 gagagtaaat gggagcttgt tcttccggct ttgcatggcg gcgaaaaata tgcgcgacat
    80641 gccagctatc cttgcggtta agtacatacg gcagatggta acgggtggtg cttttgccga
    80701 agccaataaa ggcgcagttg atgaccatga tttcgtcctt tttaaggtgg ttatttacac
    80761 cttagcgcaa agcggacgtg gttcctactg gagtgcgcat aacgaacaca agcacttccc
    80821 gaggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg ctaagtaata
    80881 ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct tcctgatcgc
    80941 gagacaataa taatcattct cattcgcact tgtccaacac tttttgcaaa aaaatgcatt
    81001 tgactcgcat tcgaaagtca atgatgttga aagggacatt taccccaaag aggacaaagg
    81061 aatgagtgag atagtgatac gccatgctga aacacgggat tacgaggcca tcaggcagat
    81121 tcacgcccag ccggaggtgt attacaacac actacaggtg cctcatcctt ccgatcatat
    81181 gtggcaggag cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct gtattgatgg
    81241 agacgtcgtg ggccatctca ccattgacgt gcaacagcgc ccacgccgca gtcatgttgc
    81301 cgattttggt atctgtgtcg acgcccgctg gaagaatcgc ggcgtcgcca gcgccctgat
    81361 gcgagagatg attgaaatgt gcgacaactg gttgcgggta gatcgtattg agctaacggt
    81421 gttcgtcgat aacgcgccag caattaaggt ctataaaaaa ttcggctttg agattgaagg
    81481 gactggcaag aagtacgcat tacgtaatgg tgaatatgtt gatgcgtatt ttatggcgcg
    81541 ggtgaagtaa agaatattct caaattgcct gttaccaatg tgaataatta gttttgttca
    81601 tctaatttga gtttttcctt atattaccac catcattttg aaacaatttg taacatcaag
    81661 agacctttga atcattactc gttaatattt cttattaaag agaacatatt ttcgcaagca
    81721 atagttatta ctgtttgttt atggatagag agtctactta atgggtacag cagctataag
    81781 tcatttacga tatgatctta acaaatatgc cctttcttta cgcaaaaccg caacacttgc
    81841 ttcaacattt tttattgagt ccccattagt tagattcgag tatctgcaag a
//
LOCUS       DAZGXS010000005        80502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGXS010000005 DAZGXS010000000
VERSION     DAZGXS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            125..1822
                     /gene="ypdA"
                     /locus_tag="ACHKG5_000403"
     CDS             125..1822
                     /gene="ypdA"
                     /locus_tag="ACHKG5_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0866585.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1837..2571
                     /gene="ypdB"
                     /locus_tag="ACHKG5_000404"
     CDS             1837..2571
                     /gene="ypdB"
                     /locus_tag="ACHKG5_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP0866586.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2584..3441
                     /gene="ypdC"
                     /locus_tag="ACHKG5_000405"
     CDS             2584..3441
                     /gene="ypdC"
                     /locus_tag="ACHKG5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0866587.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3444..5939)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_000406"
     CDS             complement(3444..5939)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_000406"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0866588.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5964..7001)
                     /gene="ypdE"
                     /locus_tag="ACHKG5_000407"
     CDS             complement(5964..7001)
                     /gene="ypdE"
                     /locus_tag="ACHKG5_000407"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0866589.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7001..8086)
                     /gene="ypdF"
                     /locus_tag="ACHKG5_000408"
     CDS             complement(7001..8086)
                     /gene="ypdF"
                     /locus_tag="ACHKG5_000408"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0866590.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8101..9348)
                     /gene="fryC"
                     /locus_tag="ACHKG5_000409"
     CDS             complement(8101..9348)
                     /gene="fryC"
                     /locus_tag="ACHKG5_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0866591.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9370..9696)
                     /gene="fryB"
                     /locus_tag="ACHKG5_000410"
     CDS             complement(9370..9696)
                     /gene="fryB"
                     /locus_tag="ACHKG5_000410"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0866592.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9915..10880)
                     /gene="glk"
                     /locus_tag="ACHKG5_000411"
     CDS             complement(9915..10880)
                     /gene="glk"
                     /locus_tag="ACHKG5_000411"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0866593.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11084..12340
                     /gene="yfeO"
                     /locus_tag="ACHKG5_000412"
     CDS             11084..12340
                     /gene="yfeO"
                     /locus_tag="ACHKG5_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP0866594.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12455..12781
                     /gene="ypeC"
                     /locus_tag="ACHKG5_000413"
     CDS             12455..12781
                     /gene="ypeC"
                     /locus_tag="ACHKG5_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0866595.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12921..14159)
                     /gene="mntH"
                     /locus_tag="ACHKG5_000414"
     CDS             complement(12921..14159)
                     /gene="mntH"
                     /locus_tag="ACHKG5_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0866596.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14495..15697
                     /gene="nupC"
                     /locus_tag="ACHKG5_000415"
     CDS             14495..15697
                     /gene="nupC"
                     /locus_tag="ACHKG5_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0866597.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15747..17936)
                     /gene="pdeA"
                     /locus_tag="ACHKG5_000416"
     CDS             complement(15747..17936)
                     /gene="pdeA"
                     /locus_tag="ACHKG5_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0866598.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18144..18219)
                     /locus_tag="ACHKG5_000417"
     tRNA            complement(18144..18219)
                     /locus_tag="ACHKG5_000417"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18184..18186),aa:Ala,seq:ggc)
     gene            complement(18259..18334)
                     /locus_tag="ACHKG5_000418"
     tRNA            complement(18259..18334)
                     /locus_tag="ACHKG5_000418"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18299..18301),aa:Ala,seq:ggc)
     gene            18555..18914
                     /gene="yfeC"
                     /locus_tag="ACHKG5_000419"
     CDS             18555..18914
                     /gene="yfeC"
                     /locus_tag="ACHKG5_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0866599.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18916..19308
                     /gene="yfeD"
                     /locus_tag="ACHKG5_000420"
     CDS             18916..19308
                     /gene="yfeD"
                     /locus_tag="ACHKG5_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0866600.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19348..20556)
                     /locus_tag="ACHKG5_000421"
     CDS             complement(19348..20556)
                     /locus_tag="ACHKG5_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0866601.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20564..20989
                     /gene="tnpA"
                     /locus_tag="ACHKG5_000422"
                     /pseudo
     CDS             20564..20989
                     /gene="tnpA"
                     /locus_tag="ACHKG5_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21107..22522)
                     /gene="gltX"
                     /locus_tag="ACHKG5_000423"
     CDS             complement(21107..22522)
                     /gene="gltX"
                     /locus_tag="ACHKG5_000423"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0866602.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22781..22856
                     /locus_tag="ACHKG5_000424"
     tRNA            22781..22856
                     /locus_tag="ACHKG5_000424"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22814..22816,aa:Val,seq:tac)
     gene            22901..22976
                     /locus_tag="ACHKG5_000425"
     tRNA            22901..22976
                     /locus_tag="ACHKG5_000425"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22934..22936,aa:Val,seq:tac)
     gene            23023..23098
                     /locus_tag="ACHKG5_000426"
     tRNA            23023..23098
                     /locus_tag="ACHKG5_000426"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23056..23058,aa:Val,seq:tac)
     gene            23103..23178
                     /locus_tag="ACHKG5_000427"
     tRNA            23103..23178
                     /locus_tag="ACHKG5_000427"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23136..23138,aa:Lys,seq:ttt)
     gene            23298..23639
                     /locus_tag="ACHKG5_000428"
     CDS             23298..23639
                     /locus_tag="ACHKG5_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP0866603.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23630..24556)
                     /gene="yfeR"
                     /locus_tag="ACHKG5_000429"
     CDS             complement(23630..24556)
                     /gene="yfeR"
                     /locus_tag="ACHKG5_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0866604.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24646..25644
                     /gene="yfeH"
                     /locus_tag="ACHKG5_000430"
     CDS             24646..25644
                     /gene="yfeH"
                     /locus_tag="ACHKG5_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0866605.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25641..25859)
                     /gene="ypeB"
                     /locus_tag="ACHKG5_000431"
     CDS             complement(25641..25859)
                     /gene="ypeB"
                     /locus_tag="ACHKG5_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0866606.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25861..27876)
                     /gene="ligA"
                     /locus_tag="ACHKG5_000432"
     CDS             complement(25861..27876)
                     /gene="ligA"
                     /locus_tag="ACHKG5_000432"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0866607.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27947..28945)
                     /gene="zipA"
                     /locus_tag="ACHKG5_000433"
     CDS             complement(27947..28945)
                     /gene="zipA"
                     /locus_tag="ACHKG5_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0866608.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29175..29936
                     /gene="cysZ"
                     /locus_tag="ACHKG5_000434"
     CDS             29175..29936
                     /gene="cysZ"
                     /locus_tag="ACHKG5_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0866609.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30121..31092
                     /gene="cysK"
                     /locus_tag="ACHKG5_000435"
     CDS             30121..31092
                     /gene="cysK"
                     /locus_tag="ACHKG5_000435"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0866610.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31476..31733
                     /gene="ptsH"
                     /locus_tag="ACHKG5_000436"
     CDS             31476..31733
                     /gene="ptsH"
                     /locus_tag="ACHKG5_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0866611.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31778..33505
                     /gene="ptsI"
                     /locus_tag="ACHKG5_000437"
     CDS             31778..33505
                     /gene="ptsI"
                     /locus_tag="ACHKG5_000437"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0866612.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33546..34055
                     /gene="crr"
                     /locus_tag="ACHKG5_000438"
     CDS             33546..34055
                     /gene="crr"
                     /locus_tag="ACHKG5_000438"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0866613.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34098..34949)
                     /gene="pdxK"
                     /locus_tag="ACHKG5_000439"
                     /gene_synonym="thiJ"
     CDS             complement(34098..34949)
                     /gene="pdxK"
                     /locus_tag="ACHKG5_000439"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0866614.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35054..35421
                     /gene="yfeK"
                     /locus_tag="ACHKG5_000440"
                     /pseudo
     CDS             35054..35421
                     /gene="yfeK"
                     /locus_tag="ACHKG5_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35424..36335)
                     /gene="cysM"
                     /locus_tag="ACHKG5_000441"
     CDS             complement(35424..36335)
                     /gene="cysM"
                     /locus_tag="ACHKG5_000441"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0866615.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36469..37566)
                     /gene="cysA"
                     /locus_tag="ACHKG5_000442"
     CDS             complement(36469..37566)
                     /gene="cysA"
                     /locus_tag="ACHKG5_000442"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0866616.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEVSPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37556..38431)
                     /gene="cysW"
                     /locus_tag="ACHKG5_000443"
     CDS             complement(37556..38431)
                     /gene="cysW"
                     /locus_tag="ACHKG5_000443"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0866617.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38431..39264)
                     /gene="cysT"
                     /locus_tag="ACHKG5_000444"
     CDS             complement(38431..39264)
                     /gene="cysT"
                     /locus_tag="ACHKG5_000444"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0866618.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39264..40280)
                     /gene="cysP"
                     /locus_tag="ACHKG5_000445"
     CDS             complement(39264..40280)
                     /gene="cysP"
                     /locus_tag="ACHKG5_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0866619.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40438..41229)
                     /gene="ucpA"
                     /locus_tag="ACHKG5_000446"
     CDS             complement(40438..41229)
                     /gene="ucpA"
                     /locus_tag="ACHKG5_000446"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0866620.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41358..42215)
                     /gene="murR"
                     /locus_tag="ACHKG5_000447"
     CDS             complement(41358..42215)
                     /gene="murR"
                     /locus_tag="ACHKG5_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0866621.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42379..43275
                     /gene="murQ"
                     /locus_tag="ACHKG5_000448"
     CDS             42379..43275
                     /gene="murQ"
                     /locus_tag="ACHKG5_000448"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0866622.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43279..44703
                     /gene="murP"
                     /locus_tag="ACHKG5_000449"
     CDS             43279..44703
                     /gene="murP"
                     /locus_tag="ACHKG5_000449"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0866623.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44708..46012
                     /gene="pbp4b"
                     /locus_tag="ACHKG5_000450"
     CDS             44708..46012
                     /gene="pbp4b"
                     /locus_tag="ACHKG5_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0866624.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46070..46969)
                     /gene="yfeX"
                     /locus_tag="ACHKG5_000451"
     CDS             complement(46070..46969)
                     /gene="yfeX"
                     /locus_tag="ACHKG5_000451"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0866625.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47065..47640)
                     /gene="yfeY"
                     /locus_tag="ACHKG5_000452"
     CDS             complement(47065..47640)
                     /gene="yfeY"
                     /locus_tag="ACHKG5_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0866626.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47701..48150)
                     /gene="yfeZ"
                     /locus_tag="ACHKG5_000453"
     CDS             complement(47701..48150)
                     /gene="yfeZ"
                     /locus_tag="ACHKG5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP0866627.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48137..48562)
                     /gene="ypeA"
                     /locus_tag="ACHKG5_000454"
     CDS             complement(48137..48562)
                     /gene="ypeA"
                     /locus_tag="ACHKG5_000454"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0866628.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48776..49645
                     /gene="amiA"
                     /locus_tag="ACHKG5_000455"
     CDS             48776..49645
                     /gene="amiA"
                     /locus_tag="ACHKG5_000455"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0866629.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49649..50548
                     /gene="hemF"
                     /locus_tag="ACHKG5_000456"
     CDS             49649..50548
                     /gene="hemF"
                     /locus_tag="ACHKG5_000456"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0866630.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50554..51606)
                     /gene="eutR"
                     /locus_tag="ACHKG5_000457"
     CDS             complement(50554..51606)
                     /gene="eutR"
                     /locus_tag="ACHKG5_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0866631.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51652..52152)
                     /gene="eutK"
                     /locus_tag="ACHKG5_000458"
     CDS             complement(51652..52152)
                     /gene="eutK"
                     /locus_tag="ACHKG5_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0866632.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52165..52824)
                     /gene="eutL"
                     /locus_tag="ACHKG5_000459"
     CDS             complement(52165..52824)
                     /gene="eutL"
                     /locus_tag="ACHKG5_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0866633.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52834..53721)
                     /gene="eutC"
                     /locus_tag="ACHKG5_000460"
     CDS             complement(52834..53721)
                     /gene="eutC"
                     /locus_tag="ACHKG5_000460"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0866634.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53742..55103)
                     /gene="eutB"
                     /locus_tag="ACHKG5_000461"
     CDS             complement(53742..55103)
                     /gene="eutB"
                     /locus_tag="ACHKG5_000461"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0866635.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGPALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55115..56518)
                     /gene="eutA"
                     /locus_tag="ACHKG5_000462"
     CDS             complement(55115..56518)
                     /gene="eutA"
                     /locus_tag="ACHKG5_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0866636.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56515..57741)
                     /gene="eutH"
                     /locus_tag="ACHKG5_000463"
     CDS             complement(56515..57741)
                     /gene="eutH"
                     /locus_tag="ACHKG5_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0866637.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57841..59028)
                     /gene="eutG"
                     /locus_tag="ACHKG5_000464"
     CDS             complement(57841..59028)
                     /gene="eutG"
                     /locus_tag="ACHKG5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0866638.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59018..59854)
                     /gene="eutJ"
                     /locus_tag="ACHKG5_000465"
     CDS             complement(59018..59854)
                     /gene="eutJ"
                     /locus_tag="ACHKG5_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0866639.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHVATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59865..61268)
                     /gene="eutE"
                     /locus_tag="ACHKG5_000466"
     CDS             complement(59865..61268)
                     /gene="eutE"
                     /locus_tag="ACHKG5_000466"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0866640.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61280..61567)
                     /gene="eutN"
                     /locus_tag="ACHKG5_000467"
     CDS             complement(61280..61567)
                     /gene="eutN"
                     /locus_tag="ACHKG5_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0866641.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61674..61967)
                     /gene="eutM"
                     /locus_tag="ACHKG5_000468"
     CDS             complement(61674..61967)
                     /gene="eutM"
                     /locus_tag="ACHKG5_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0866642.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(62006..63022)
                     /gene="pta"
                     /locus_tag="ACHKG5_000469"
     CDS             complement(62006..63022)
                     /gene="pta"
                     /locus_tag="ACHKG5_000469"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0866643.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(63019..63750)
                     /gene="eutT"
                     /locus_tag="ACHKG5_000470"
     CDS             complement(63019..63750)
                     /gene="eutT"
                     /locus_tag="ACHKG5_000470"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0866644.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63747..64448)
                     /gene="eutQ"
                     /locus_tag="ACHKG5_000471"
     CDS             complement(63747..64448)
                     /gene="eutQ"
                     /locus_tag="ACHKG5_000471"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0866645.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64423..64902)
                     /gene="eutP"
                     /locus_tag="ACHKG5_000472"
     CDS             complement(64423..64902)
                     /gene="eutP"
                     /locus_tag="ACHKG5_000472"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0866646.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(64915..65250)
                     /gene="eutS"
                     /locus_tag="ACHKG5_000473"
     CDS             complement(64915..65250)
                     /gene="eutS"
                     /locus_tag="ACHKG5_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0866647.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(65543..67822)
                     /gene="maeB"
                     /locus_tag="ACHKG5_000474"
     CDS             complement(65543..67822)
                     /gene="maeB"
                     /locus_tag="ACHKG5_000474"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0866648.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADCDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            68111..69061
                     /gene="tal"
                     /locus_tag="ACHKG5_000475"
     CDS             68111..69061
                     /gene="tal"
                     /locus_tag="ACHKG5_000475"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0866649.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            69081..71084
                     /gene="tkt"
                     /locus_tag="ACHKG5_000476"
     CDS             69081..71084
                     /gene="tkt"
                     /locus_tag="ACHKG5_000476"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0866650.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(71179..72222)
                     /gene="ypfG"
                     /locus_tag="ACHKG5_000477"
     CDS             complement(71179..72222)
                     /gene="ypfG"
                     /locus_tag="ACHKG5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP0866651.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(72348..72923)
                     /gene="nudK"
                     /locus_tag="ACHKG5_000478"
     CDS             complement(72348..72923)
                     /gene="nudK"
                     /locus_tag="ACHKG5_000478"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0866652.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(72991..74970)
                     /gene="aegA"
                     /locus_tag="ACHKG5_000479"
     CDS             complement(72991..74970)
                     /gene="aegA"
                     /locus_tag="ACHKG5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0866653.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            75176..76876
                     /gene="narQ"
                     /locus_tag="ACHKG5_000480"
     CDS             75176..76876
                     /gene="narQ"
                     /locus_tag="ACHKG5_000480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0866654.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            77040..80153
                     /gene="acrD"
                     /locus_tag="ACHKG5_000481"
     CDS             77040..80153
                     /gene="acrD"
                     /locus_tag="ACHKG5_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP0866655.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(80252..80311)
                     /gene="ypfM"
                     /locus_tag="ACHKG5_000482"
     CDS             complement(80252..80311)
                     /gene="ypfM"
                     /locus_tag="ACHKG5_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0866656.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        18942 a        22029 c        20495 g        19036 t
ORIGIN      
        1 tgctaataca ccatttatct atcaatcccc ttttaaaatt gtggtttttc ctcaaaaacg
       61 gcctgctggt catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc
      121 acccgtgcac gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct
      181 tatctgcctg ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc
      241 acactcaccg aaagagttgc tcgccgttac cgccattttc tcgctgttcg ccctcttcag
      301 cacctggtcc ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat
      361 gtccggcggg attctgtttg gcccgtgggt gggcatcatt accggcgtga ttgcgggtat
      421 tcatcgttat ttaattgata tcggtggcgt gacggcgatc ccctgcttta tcaccagcat
      481 tctggctggt tgtatatcgg gctggatcaa cctgaaaatc cccaaagcac agcgctggcg
      541 cgtcggtatt ctcggcggca tgttgtgtga aacgttgacc atgattctgg tgattgtctg
      601 ggcaccaact accgcgttgg ggatcgatat cgtctctaaa atcgggattc caatgatcct
      661 cggcagcgtc tgtatcggct ttattgtgct tctagtgcaa agcgttgagg gcgaaaaaga
      721 ggccagcgcc gcgcggcagg ccaagttggc gctggatatc gccaacaaaa cgctacctct
      781 gtttcgccat gtcaatagcg agtcattacg caaagtttgt gaaattattc gcgatgacat
      841 tcacgccgat gcggtggcga ttaccaatac cgatcatgtg ctggcctatg ttggtgtggg
      901 tgaacataac tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa
      961 ttacggaaaa atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc
     1021 catgctggtg atcccattgt gggagaaagg cgttgtgacc ggaacgctga aaatttacta
     1081 ctgccacgcg catcagataa cctcgtcatt acaggaaatg gcggttggtc tgtcgcaaat
     1141 catctccacg caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc
     1201 agagcttcgc gccctgcaaa gcaaaattaa tccccatttc ctgtttaacg ctttgaacgc
     1261 tatttcatcg tcgatccgtc tgaatccgga taccgctcgc cagctgatct ttaatctgtc
     1321 gcgttatctg cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga
     1381 gctgtatcaa attaaagact atattgccat tgagcaggcc cgctttggcg acaagctgac
     1441 ggttatctat gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc
     1501 gttggtggag aatgccattg tccacggtat ccagccttgc aaaggtaaag gcgttgtcac
     1561 catcagcgtt gcagagtgcg gcaatcgggt acgcattgcg gtgcgggata ccgggcacgg
     1621 catcgatcca aaggtgattg agcgggtcga agcgaatgaa atgccgggca ataaaattgg
     1681 cctgctgaat gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg
     1741 cctggaacca ggaacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc
     1801 acaggctacg ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac
     1861 gaattcctgg cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt
     1921 gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac
     1981 gccatttttc tggatatcaa tattccgtcg ctggatggtg tgttgctggc acaaaacatc
     2041 agccagttcg cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggta
     2101 gaagcgtttg aactggaggc gtttgactac attctaaaac cgtatcagga gtcacgcatt
     2161 accgggatgc tgcaaaaact ggaagcagcc tggcaacaac agcagaccag cagcacgcct
     2221 gccgcgacgg taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc
     2281 gtcacgccaa ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtttat
     2341 acgcggcgtg aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg
     2401 ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatccgcgaa
     2461 atcgaaccgt ggtttaataa cacctatatt ctgcggctga aagatctgga ttttgaagtg
     2521 ccggtcagcc gcagcaaagt taaagaattc cgccagttaa tgcatcttta atcgggaact
     2581 ttcatgaaag caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc
     2641 ctggtgctta acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgttg
     2701 ccggtgggca gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac
     2761 ggcaatctgc tggaagcaaa gcagcaacgt ttggtggaag gagaaatgct gtttattccg
     2821 gcgcgcgcgg ctaatttacc ggtcaacaac aaaccggtga tgctgttaag cctggtgttc
     2881 gctccgacct ggcttgggtt atcgttttac gatagccgca ccacgtcatt gttgcatcct
     2941 gctcgccaga tccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc
     3001 ctcacccatc ttagccgttc gccgctggag caaaatatca ttcagccgct ggtgttaagt
     3061 ttgctgcatc tttgccgtaa cgtggtgaat atgccgccgg gcaattcgca gccgcgcggc
     3121 gattttctct atcacagcat ttgtaactgg gttcaggata attatgccca gccgctcacc
     3181 cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct
     3241 cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg
     3301 atgattttgc agaaatatca tttgtcaatt catgaagtgg cacagcgttg cggttttccg
     3361 gatagcgact atttttgtcg cgttttccgg cgtcagtttg gtctgacgcc gggagagtac
     3421 agcgcccgtt ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata
     3481 ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc
     3541 gccagctgcg agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg
     3601 atcaccagtt cgacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca
     3661 atgctgatac tggaatgacg gatccactga gatttggtgt gcgggatcgc cacgccaaaa
     3721 ccaacgccag tggtgacaat ctcttcccgc tgccagacat cctcttccag ctcgaacgga
     3781 tgttcagtgc gcccgttaac gccgaggtta ccgcacagga actggatcgc ctgctcttta
     3841 ttgctaaagg cctgatcaac gaagatattc tccagcgcca gcagcgggcg aacgtcttct
     3901 tccggcgtaa aggcggtgag taacgcttca atttcctgcg cgctgcgaca ttcacacgcc
     3961 tgacgtgcca gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc
     4021 ggaatgcgcg ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga
     4081 taacggcttt caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca
     4141 gtggtaacta tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc
     4201 gatacgcgcg ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg
     4261 atactgaaga aatcgacctc atcgcagaag tgatcgatga tgtagcacac cgacggcact
     4321 tccaccatga tacccagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca
     4381 acgatcgctt tttggatctc gcccttcacc cataagatct gatcgaggcc gtggaccatc
     4441 gggatcatca actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg
     4501 cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaacgggttc
     4561 tcttcctggg gaatattcag ataaggaatg cttttatcgc ctccgatgtc cattgtgcgg
     4621 aagataatcg gcttgtcgcc cgctgccagt agcacctgct ggtaggcttc aaattgctcc
     4681 tgctcgtccg gctcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgacg
     4741 ccttccgcgc cgttggcaaa cgcgccaggc gcttccagag cggtgccaat attcgccgcg
     4801 atatcaatac gcttgttatc acgggaataa gccagctgcg cggcagcctg cgcctgttgt
     4861 ttttggcgtt tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc
     4921 gggttaatcg ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgc
     4981 gcgatcgcat ccagcggcag gccgctcagt accgggatcg ccgaggcgcg ggccagaatc
     5041 agggtatgcg aggtgcgccc ggttttctcc agaatcatgc ccgcaagatt ttttaaatcc
     5101 aggcttaaaa actggcttgg ggttaaatcc tcggccacca gaatagtcgg tttttccagc
     5161 accagattgt tgcgcggctt cagttccggc caggtaatat gcagcaactg ttcgctaata
     5221 tcgcgaatgt cgctaacacg ttcgcgcaga taatcgctgg cagaagcaga aagtttggcg
     5281 caaacctgct ccatatttct gatgatcgcc gcccccagcc cctgatgctg ttctgtcatc
     5341 aggcgacgga tattgcctgc aaattcatca tcctgaatca gcgacaaatg ggcgctgagg
     5401 atagttttgc tttcgccgtc acgctcacgc aattgctggt tcagttgctc ggcaagcgtt
     5461 gccaggctgt gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg
     5521 tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca
     5581 ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc
     5641 agttctgcct gcgtaggctg aacgctgtcg ctgtcgataa agcgcacctg gatgtactct
     5701 tccagcacgc gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca
     5761 ttaaataggg tgccggtgcc aatcagcgcc agcgagcttt tggcatcggc ctttgcgttc
     5821 tggcgatggt taataaaagt gatttcgctt tgccactggc tgcactgttc tttaagttcc
     5881 cacgccggac gagcatgtag accgttaggc aaaggacaga gaaattgaat cgttaacata
     5941 accgctccgg gttaatcagg agatcatctg aaatccgtca gttgaacaac cgtctcacgc
     6001 gtaagacgtt gaataagggc agataaaagt tgctccatct gcaaaatgtc acggcaatcg
     6061 gcaatcgacg cggcgcaatg tccgtggcgg gtggcaggcc ccatcaccac tgtgggtacg
     6121 ccagtaccgg ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa catatccgcc
     6181 tgtaacggca cgccaatttc tgccgccacg gtttcgatcc aggcggtgag ttttggcggc
     6241 gcaatcagtg acttgtcgct taacaccagc atcgggccgt taccaatctg gcgatggtta
     6301 gccgcgccat aattaaagtt tttcgcccag caggcggtat caaggacaat ggcgacgtcc
     6361 ggcgacaccg cgcgggtggc agtttgcccg ccgcgtaacc ccacctcttc gctggaactg
     6421 gccaccagcc acacttccgc aggcagttca gcgctgtgta gttcgcgcag taacgtcacc
     6481 agcaggtagc aaccgaggcg gtcatcaaag gctttcccca tcacccgctg gtgggggaga
     6541 acctgaaaag tggtatcgaa cgtgacgcga tcgcctggac gaattcccgc ctgcatcact
     6601 tcgtcatacg agcgcgcgcc aatatccacg cgcatggcgc tgacatcatt cccctgccgg
     6661 tcgccgtcaa gcaggcctgg aattttgcac tcttcacggg tggtgatgcg caccggctgc
     6721 agctggcggg cagccatgcg tacattgcca accggcagca catcaatcgc cccttcgcgg
     6781 gagatgctgc gcaccataaa tcccacttcg tccatatgcg cacagatcat caccttcgga
     6841 cctgtcgatt cattgaggcg gatcagcacc gatcccagcc catcaaatcg cacttctttt
     6901 tgcaggcgat ccgcttcttc cagcaggatc tgccgcactt cctgttccga ggaggcgatc
     6961 gcatctgcct cgctcaacgc tttcaatagc gataaatcca ttatgcctct cccgtgagca
     7021 acactgtttt cggcatggcg tagagcactt ctgcgccttg cggggtgacc agcacaacat
     7081 cctcgatgcg cacgccccct tgccctggca aataaatccc cggctccacg gtcagtaaca
     7141 tgcctggctg tagcgtcgtg gtgtcccgcg gtgaaaaacg cggatcttca tgaacttcaa
     7201 tgccgatagc atgagcggtg ttatgaccga aatagtggct aaaacctgcc tcggtaatca
     7261 cccgacgcgc ggcttcgtca acctgctggc agcgcacgcc ggggcgaatt gcagagattg
     7321 ctgcgagctg tgcctgcaaa acaatctgat agacgttaaa cagcgggtga gattcggcgc
     7381 tcaccccttc gccattcacc agcaaggtgc gcgtcatatc agagcagtag ccctgataga
     7441 gcgcaccgaa atcgagagtg acaaactcgc ccgctgcaac aatcttgtcg ctggctttgc
     7501 cgtgcggcag cgccccacgc cagccactgg cgacaatggt gtcaaaagag gttttttctg
     7561 cgccctgctg gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg
     7621 cctgaataaa gcggcgaata tgctctgcac cgcgatcggc aatcccacag gcaaggcgga
     7681 ttttctccac ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca
     7741 gtttcgcatt gagttcagac tgccagcgat gcgcggtttc ccaactcacc tgctggcctt
     7801 caaaaccgag cgtttgcaac tgctcatcgg caatgatttg cctggcgatg gttgtaagcg
     7861 tgtgcgtcgc gtcaagcaaa tgcagctggt aaccttgcgt gcgggcttct acatccgcgt
     7921 aatagcgcga gtccaccaga atgtgcgcac tttcacggct aatcagcaca tagcctgatc
     7981 cggtggagat ccccagatgc ggctgtttgt tctgccgtga ggagagaagc accgcatcca
     8041 gttgttgcgc cttaagccag tcgcgcagcg aagcgagtaa tgtcatctgg attttccttc
     8101 ttacaggcta tcgattaaca atttgccttt acggaacatc atcagacgca ggaacaccac
     8161 catcagcgca gttatgactg cccccagcgc gatccccgcc atatagacgc ccaggttagt
     8221 gaccagcggc catgcccaga tagcggattc cgggaaccat tgcactgcgc ccagccagac
     8281 agcggcggtt gagccgacaa tcgcgccgac catatacgac ggaatggcgg tgatggggct
     8341 ttccagcgca aacggaatcg caccctcact gatccccata aaggcaagga acatcgcggt
     8401 cttaccctgc ggataaagtt gtgcgttgaa caggcgtttg ccggttaaac gacggtcaat
     8461 aatggtcgcc agacccagac caatcggcgg aataacgata gcgatagaac gagcggtgac
     8521 cggtaatacg tggtcagtgg taaagctgaa ggcaacaaaa cctgcggctt tgttgatcgg
     8581 gccaccaagg tcgattgctg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt
     8641 ctcacctgcg gcagtcagca cagtacggat accgccgttg atccagcccc caaagggggt
     8701 gatgacgtag tacatcgcca gcatgacaaa aatagcggaa agaatcggta gcaaaaatgt
     8761 ggttttgaac gctaacagga aatccggtaa ctggattttc tggttcatcc atttcacaag
     8821 gtaaccggca acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga
     8881 ggtcgcccat tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat
     8941 caagccgccg ataaaaccag ccggaaacgc cagtttgcca ccgatagagt tcgccacaaa
     9001 agcagcaaac atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacaacca
     9061 ggcaaatttc agtaaggaga ggtcaaaacc ggagaatttc ccgctattaa gagcatccat
     9121 gatgccgatc tcagcaggaa ttttaagcca gctataagca atcaactggc tgaaagcgag
     9181 gatcacaccg cccataatta aggtcggcac catgcgtgaa atccctgaca tcacatgctg
     9241 cgggagttca ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc
     9301 accggatgcg ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc
     9361 tgtcgaatat tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc
     9421 agcgttttta attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgttc
     9481 gttatcttcc ggggtaactg ccacggagtg gatgatgatg gtcgcttcgg cgatatcctg
     9541 cgccgtcagg cgattctgga taccgtccgc gccctgggtt tcaattttca cttcataacc
     9601 ggcttctacc gccgcttctt ccagcgcctg agcggccata aaagtgtgag ccaggcccat
     9661 tgggcaggca cataaggcaa tcagtttctt actcatcgtt aattcctcgt tcagtgatag
     9721 ttgacgagtg aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt
     9781 tactggagaa ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgaggggaca
     9841 caaagaaaag ttgaattaac tgtatgattt aaaagattat cgggagagtt acctcccgat
     9901 ataacaggaa ggatttacag aatgtgacct aaggtctggc gtaaatgtgc accggaaccg
     9961 agaagacccg gattgtcatg gacgatgaga tacaccggaa tatcatggac atattcttta
    10021 aagcgccctt tatcttcaaa tgcggcacgg aaaccagagg ctttgaagaa ctcaaggaag
    10081 cgcggcacga taccgcccgc aataaacacg ccgccaaatg tcccgagatt gagcgccaga
    10141 ttgccgccaa aacggcccat aatgacgcaa aacagcgaca acgcacggcg gcaatcggtg
    10201 cagctgtcag ccagcgcgcg ttcggtaata tcttttggct tgagattttc tggcaggcgg
    10261 ttgtcggctt taacaattgc gcgatacaaa ttcaccagtc ctgggccaga aagcacgcgt
    10321 tccgccgaaa catgaccaat ttccgcacgc aatatttcga ggataatggc ctcttcttca
    10381 ctattcggcg caaaatcaac gtgaccgcct tcgcccggca agcttaccca acgcttatcg
    10441 acatggacca gatgcgcaac cccaagcccc gttccggcac cgtaaaccgc aataggctta
    10501 ccttctaccg gttctgcgcc accaaactga atcagatgct cttttttcag catcgggatc
    10561 gccatcgata cagcggtaaa atcgttaata atttccagat ggctaaaacc gagattcttt
    10621 ttcatttcgg caattgagaa cgcccaggta tggttggtca ttgccaccca gtctccggta
    10681 atcgggcaag cgatggcaat acagccgtct ttcacctcga ccttatgttc ttcaagataa
    10741 acgcgaatga ccgcttcgag gctggggtaa tcaagccctg aataggtctt agcctgcgag
    10801 atttcaccac tggcaatatc acacagagca agacgtgcgt tggtgccgcc cacatcaccg
    10861 actaatgcat actttgtcat tcttcaactg ctccgctaaa gtcaaaataa ttctttctca
    10921 cactgtaaat acctgggggc ataacaacaa cgccggaaag gcaggctccc tgtaaatatc
    10981 gatctgggtc acacaattac tttatcgttt cagcaccaat tgcagcgatg cctttttgca
    11041 agctgggcaa actaagtatc tgaccccgca taaggaatag aacatgctcc atccgcgagc
    11101 cagaaccatg ttgttattat cgctccccgc cgtggcaatt gggattgcgt ccagtcttat
    11161 tctgattgtg gtgatgaaaa tcgcctcggt attacagaat ttgctctggc aacgactgcc
    11221 gggaactctg gggatagccc aggattcacc cctctggatc atcggtgtat taacgctaac
    11281 gggtattgcg gtggggttgg ttatccgttt cagccagggt catgccggac cagaccccgc
    11341 ctgtgaaccg ctgatcggcg caccggttcc gccctctgcg ctaccaggac ttatcgtagc
    11401 attaattctc ggtcttgctg gcggcgtcag cctggggccg gaacatccga tcatgaccgt
    11461 caatatcgcc cttgcggttg ccattggcgc tcgtctgtta ccgcgcgtca accgaatgga
    11521 gtggactatt ttagcctctg ccggaaccat cggcgcgctg tttggtacac ctgttgcggc
    11581 ggcgttgata ttttcgcaaa ccttaaatgg cagtagtgaa gttccgctat gggatcgtct
    11641 ttttgcgccg ttaatggcgg cagcagctgg tgcacttact accggattat ttttccatcc
    11701 ccatttttca ctgcccattg cgcattacgg acagatggaa atgaccgata ttctcagcgg
    11761 tgcaattgtc gcggcgattg ccatcgcagc agggatggtc gccgtatggt gcttaccacg
    11821 gttgcacgcg atgatgcatc aaatgaaaaa tccggtgctc gtgctgggta ttggcggatt
    11881 tattctcggt attctggggg ttattggtgg accagtttcg ctgtttaaag ggctggatga
    11941 gatgcagcag atggtggcaa atcaggcttt cagcaccagc gattactttt tgctggcggt
    12001 aattaaactt gccgccctgg tcgttgctgc cgccagtggc tttcgcggtg ggcgaatctt
    12061 cccggcagtg tttgtcggcg tggcattagg gttgatgctg catgagcacg ttcccgccgt
    12121 accagcggca ataaccgttt cctgcgctat tctcggcatc gtgctggtgg taacacgcga
    12181 tggctggtta agtcttttta tggcggcagt cgttgtaccc aataccacat tgctaccgct
    12241 gctctgtatc gtcatgcttc cggcatggct gttattagca ggtaagccga tgatgatggt
    12301 caatcgtccg aagcaacagc caccccacga taacgtttag caaaaaatgc tctcctttat
    12361 gatttaagag ttatggcgat gatacggggc tttacctccc cgtaatattg cgttaacagg
    12421 ccgctgacgg cttcgatcgt gaaggagaat aacaatgttc aggtcactgt ttctggcggc
    12481 cgccctgatg gcatttaccc cgcttgcagc aaacgcaggc gaaatcaccc tactgccatc
    12541 aatcaaatta caaattggcg atcgcgatca ttacggtaat tactgggacg gtggtcactg
    12601 gcgcgaccgt gactactggc atcgcaatta tgaatggcgt aaaaaccgct ggtggcgtca
    12661 tgataatggc taccatcgtg gctgggataa gcgtaaagca tatgagcgtg gctatcgtga
    12721 aggctggcgc gatcgtgacg atcatcgcgg aaaaggccgc ggacatggac accgccatta
    12781 aacacattcg ccaatggagc acaaggcctg atgcgacgct agtgcgtctt atcaggccta
    12841 caaaccggct ctttaacgcg tagggcggat aaggcgttta cgccgcatcg ccaatagtgc
    12901 cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaataa ccagatattc
    12961 agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt gctgttcacc
    13021 agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag cgccagggcg
    13081 ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc taatcccatc
    13141 agaatgacaa taaatgacgg cagcatggtg actgtacgac gcacccacag cgggatatgg
    13201 aagcgaatga acccctgcat caccacctgc cctgccagtg tccccaccac cgttgaggac
    13261 agtccggcag caaccagact taacccaaag accgttgccg cagcatggct taacagcggt
    13321 tgcagcgtca aataagcctc atcaagatcg gcaacaccag tatgaccaga aaagtggaac
    13381 gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatagt catggcgata
    13441 gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg taaatgctga
    13501 gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct
    13561 gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt acccagctgc
    13621 gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt
    13681 aacccgccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat taaaatcagg
    13741 aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag aatgagttta
    13801 aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc
    13861 tgaacccaat agaaccacac tacgggacgc ggatagtgat cgcgaatctg ctccgccaga
    13921 tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat cgccatcagg
    13981 ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc ctgaatattg
    14041 gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat
    14101 gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc
    14161 ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga gaatgattat
    14221 caaattcatt taaatgggtt gtggtgattt ctctgataga ccaggattat gactacgaaa
    14281 agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag
    14341 cttccataca caacatagca gaaatgtatg acagctcact atttttgaag cttgtcacag
    14401 gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca
    14461 cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact
    14521 ggcacttgcc gttgttgcga tcctcgcact gctggtaagc agcgaccgca aaaaaattcg
    14581 tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa
    14641 ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg
    14701 atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt
    14761 cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca
    14821 gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct ccaaagtcaa
    14881 cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga
    14941 aaactttatt gcctataaag atatccttgg caaaatctcc cgtaaccgta tgtacaccat
    15001 ggcagcaacg gcgatgtcca ccgtttctat gtccatcgtt ggtgcataca tgaccatgct
    15061 ggaaccaaaa tacgtcgttg cagcgctggt actgaacatg ttcagcacct ttatcgtgct
    15121 gtcgctgatc aacccttacc gtgttgatgc ctgtgaagaa aacattcaga tgtccaacct
    15181 gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaagt
    15241 tgccattatc gttgccgcaa tgctgattgg ctttatcgcc ctgatcgccg cgctgaacgc
    15301 actgtttgct accgtgactg gctggattgg ctacagcatc tccttccagg gcatcctggg
    15361 ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt
    15421 gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga tggatcttca
    15481 gaaaattgcc tctacactct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc
    15541 cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga
    15601 gcaaggtaac gtggtttctc gcttcggtct gaagctggtt tacggctcta ccctggtgag
    15661 tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac catacataaa aaagccgggg
    15721 ataattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa
    15781 tacccttgca gatattgcac cccgagctta tgcaatagcg cctgttgctg ttgcgtctcg
    15841 acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat tgatctcaca
    15901 atcatcgcat ccagcgtatt cgtgacaata tctttcacaa agacgccatc aattttgatg
    15961 atatcagcct gcaaacgctt taaccgttcg tagttggcat agccggtgcc aaagtcatca
    16021 atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct gctttctgcg
    16081 ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga gatgtgataa
    16141 cgcttaaata gacgaataat ccgcccggca atattctttt gcagcagcgt gagcggcatt
    16201 aaattaactg aaaagcgcgg gccttttttg tcgcaagggt gtgttgccag ccacttcaac
    16261 aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc aataaggggc
    16321 agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag gacctcatca
    16381 taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc accctgatct
    16441 aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc ggaaaccatc
    16501 tcctctcggc tatccagcgc cagcacatga tgatgtgcgc aggattgctc cgccagccag
    16561 cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg cccccaggca
    16621 gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact attcaggatg
    16681 ttaaccatat gctggagtcg cccttccgtt tcaggcccgc tcagcactaa cagcagttca
    16741 ctacccggca attgatacaa cttttcgttt tcctgcatca atggcagcag cgtacggtaa
    16801 attgatcgga tacagtgaac acgcatcatc aacccgtaat gacgactcat aaactcaaga
    16861 ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gctccggcgc ttgttccaac
    16921 gcacgaaagt tgggcaggag cgttaacgga tcggtcagcg cctgcaaatg ccagcggcga
    16981 ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac gctaaaggaa
    17041 atcagcaccg cgagaataaa tgccagcgaa tattcggttt ccaccccttg caaaaagttc
    17101 tggttgtaat tcagcaggca aagcgttgaa acagcccaag tgagatttaa aaacggatag
    17161 cgaagcttac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccag gtaaccggca
    17221 ataaagtcgt tttcataagg tgtgcacaac agaagtagca gcacgcttaa cgctgccagc
    17281 cagcttaagg taaatgcgcg tttctctttg cccaacgacg gagcgatatc cctgcgccac
    17341 aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa aagcatgttg
    17401 taaatcagca cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca
    17461 tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac aagatacatg
    17521 ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg ccagacatat
    17581 cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca
    17641 cacaacacga caaacgtctg gctaccgagc atgaccgcag tctgtaaaaa agagagatga
    17701 aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca
    17761 aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg
    17821 gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc
    17881 gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt
    17941 acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcattgcg
    18001 aagcaaacag gggccatcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
    18061 actaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
    18121 ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
    18181 catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa
    18241 tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc
    18301 catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg
    18361 ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca
    18421 atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt
    18481 catgtcacgg tttcctgtaa agtggtgcta taaaatgaac tactaataga cccacataca
    18541 ttcagggaat tgttatgttc aaggagagga tgacaccaga tgaacttgcc agactgaccg
    18601 gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa
    18661 aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat
    18721 atattcgtaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctt tctggtgatg
    18781 cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac
    18841 agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc
    18901 gcgatagcaa ataatatgaa aagattacgc aataaaatga ccaccgaaga actggctgaa
    18961 tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg
    19021 gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt
    19081 tgcgagttta ttcagaacac gccagccttc cataacacac cgatgcttct ggaagccgaa
    19141 gaaccccttg ccgaatacgc accggggatt cgcactcctg cttatcggca aatcatcagt
    19201 gcaatagaca acatgactca cagtgagcag gaaaaagttg cgcaattttt gtcacgcgaa
    19261 ggaattcgta atttccttgc tcgtctcgac atagatgaat cagcttgaca tcctccacgc
    19321 cctgaatgac gaggatccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc
    19381 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    19441 cgctaaaatg ttacgagcgc cgtttacatc ggcgttcgct gtatatccac atacctggca
    19501 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    19561 ctggcttgtg tacgctggcg gaacagccag tacctgaccg ccacgccaga gctgcttgta
    19621 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    19681 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    19741 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    19801 tttgtgaagg tagtccctgc ggatatttgc ggtacaggaa tgcagtcgct gtattttgcg
    19861 tttctgcttc tgccagttat tgctgaattt gaccttgcgg cttaactgtc gctgaagtct
    19921 cgccaacttc ttctggtttt tctgaaaact gtttacaggc tcaaagactg tgccatctga
    19981 cagcgtggcg agtttagcca cgccagcatc cagtccgacc attgatgctg aagggtgaac
    20041 cggagttgat acttcacttt ctgtctgaat actgatgtac cacttaccgc aggactggct
    20101 gacagtgaca tttttcacaa cacccgtgac ctgacggctg ttcaggtagc gcatccagcc
    20161 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgccct gcgggtagcg
    20221 gaatgcatca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tccggaagaa
    20281 gtttttgtaa gcccgctcaa gctctttcag tgactgttgc aatggctgtg acggggcatc
    20341 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    20401 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    20461 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccacctgg
    20521 tcttaactgg aatttaaatg cctgtagtcg tttcacaggc caaatgatac tttggtctat
    20581 gaataaagaa accgatatcc gacggggaag gcattgtgtt ttcctgatgc atgttcatct
    20641 gttctttgtc accagatacc gacgtcagat ttttgactat gacgcgacag aaaaactacg
    20701 cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa tggatggcga
    20761 accagatcac gtccatttgt taactaacta tcctcccaaa ctggcgatat ccagtctggt
    20821 aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag atattgcagt
    20881 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcggagg
    20941 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgga
    21001 aaaccgcgcc ttatatcccc gccctgaagg acggggtttt acggcgcacc ggataaataa
    21061 aaaacggcag gggtatctct cctgccgttt atcttttatg cgctaattac tgctgatttt
    21121 cgcgttccgc aataaaagcc agcgctttgt tgatacgctc gatactgcgg gtcttaccga
    21181 tcgcgtgaac ggtaacgtcc agcgccggag actgccccgc accggttacg gcgacacgca
    21241 gcggcatacc aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt
    21301 gatgaacgtt ttcagctgtc cagtcagtaa tcgcggccag tttgtcacga accacttcca
    21361 gcggctgacg cgctaccgga cgcagatgct ttttcgcggc gtcggcatcg aactcagcaa
    21421 aatcttcgta gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc
    21481 ccagcagttt caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct
    21541 caatgtgcca ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat
    21601 ggttcagcca cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca
    21661 aagtgaagta tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc
    21721 ccagacgcac cagatagttc agcagtgctt ctggcaaata accgtcatca cgatactgca
    21781 ttacgctgac tgccccgtga cgtttggaca gttttttgcc gtcatcgccg ttgatcatag
    21841 aaacgtgcgc gtaaaccggt accggcgctt tcagggcttt aagaatgttg atctggcgtg
    21901 gcgtgttgtt gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat
    21961 cgacaaccac acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt
    22021 ccagttcctg gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac
    22081 cttcctgcgg gttagcaaaa cgtacgacac acggctcatc atcagcgtga tgctcatgac
    22141 tgtggcggca gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca
    22201 gcgcttccag gcgctcttta gagcaatagc atttataagc tgtgccctct tccagcatct
    22261 gatcgatcac cgcgttgtag cgatcaaaac gtttggtctg gaagtacgga ccttcatccc
    22321 actccaggct cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac
    22381 gctcaagatc ggtgtcttca atacgcagca cgaactcacc gccgtgatta cgtgcaaaaa
    22441 gccaggagta aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg
    22501 cgaagcgagt tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc
    22561 aaatcctgga aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag
    22621 ttcgggttgc aaccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt
    22681 ttgcgatcat cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac
    22741 tcattttgaa ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg
    22801 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta
    22861 ctttatgtag tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg
    22921 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttt
    22981 ctcaccagct aaatttcttg taaaaatgtg aagtaccgaa gtgggtgatt agctcagctg
    23041 ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact
    23101 tcgggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga
    23161 atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga
    23221 tttgtaagat tgatttttct tctcaagttc cttgttcttc tgccgatatt acctcgtaca
    23281 gtgacgagga ctgaacaatg gccactatct cttcaacttc aattccttcc atacaaacac
    23341 agagtagtaa cagggcatca caaggtagcg atgttgcttc acaaatcgcg cggataagtc
    23401 agcaaatcat caaactcact caacacatta aagagattgt agacactagc ggcagtgctg
    23461 aagacaagca aaaacaggcc gaattgattc aacagcaaat caccctactg gaaacacaac
    23521 tggcgcagtt gcagagacag caggcagaaa aggcgcagga gaaagagcag cgtttgtctc
    23581 tgaatgtatc ccttctaaat cctgttgaaa atacgactca tatcggtatc tatatctgat
    23641 acaacggatc cccttcccgc ccagccatta gcgtattgcc ctggtctcgt accacgtccc
    23701 ataacgcttc agcggcggtg gaaagtgaac ggtttttcct acgcaccagc atcaactggc
    23761 gttccaccac cggcgtaatg cgctttacca ccagcgggct accttcaggc aatggtagcg
    23821 ccagtgcggg aagaatactg atgccgatgc ctgcggctac catcgggaac agcgtcgccg
    23881 gatgtccaat ctcctgcaca atattcgcct gaataccatt gcgcgccagc gctgcgtcaa
    23941 tcagcggtcg gctgcctgac gcataatcct gcaacaccaa tttcgctccc tgtaatgcct
    24001 gccagggtac ataatcttcc gcggccaaag cgctatcccg atggcaaagc agaaagaaag
    24061 gctcggaaag aatcacttca cattgcagat cgcccactgg gcctggatcg atgacgatgc
    24121 caaagtcgac atctccctgg cgaatacttt ccatcaccca ctgctgcgga cgatcgtgca
    24181 ggacaaactg aatatctgga tagcggcgat ggctttcagc aatgcattgc ggaataagat
    24241 gcgcggaaat ggtctggctg gcagcgaccc gaacttttcc gctcagttgt tgccccatac
    24301 gcccggtatc gcgcaacgtg ctgttaagtt catccagcag tcgctcaaga cgcaaagcca
    24361 actgctgccc tgcatctgta agcaccactt cacgcgtggt tctgtccagc aggcgaacac
    24421 cagtatgatt ttccagctcc ttcacactgt gacttactgc cgactggctc aggccgatac
    24481 gctctcctgc acgactaaaa cttttctcct gcgctactgt gacgaaaacc tttaattgtt
    24541 ttaaagaata attcatctgt tttcttcatg aatggatgca ataaatcaat tttatttctc
    24601 aaactggaag aagcacaata gaaccatcga tcatctggag tctttatgaa actttttcgt
    24661 atcctcgatc ctttcacctt aaccctgatc acggtggtgt tgctggcctc tttctttcct
    24721 gccagaggcg atttcgtccc cttctttgaa aatctgacca ccgcagctat tgccctgctg
    24781 ttctttatgc acggcgcgaa gttgtcgcgt gaggcgatta ttgctggcgg tggtcactgg
    24841 cgactgcatt tgtgggtaat gtgcagcacc ttcgtgctgt ttccgattct gggtgtactg
    24901 tttgcctggt ggaaaccggt aaatgtcgac ccgatgctct actccggttt tctctacttg
    24961 tgcattctcc cggctaccgt gcagtctgca atcgccttca cgtcaatggc gggcggtaac
    25021 gtcgcggcgg cggtttgttc tgcgtcagca tccagtctgc tggggatttt cctttcacca
    25081 ttgctggttg gtctggtgat gaatgttcac ggtgcagggg gcagccttga gcaggtcggt
    25141 aaaattatgc tgcaactgct gctgccgttt gtgttggggc atctttcccg gccgtggatt
    25201 ggtgactggg tgtcgcgcaa taaaaaatgg attgcgaaaa ctgaccagac gtccattctg
    25261 ttggtggttt atacagcgtt cagcgaagcc gtcgttaatg gtatctggca taaagttggc
    25321 tggggatcat tgctgtttat cgtggtggtc agctgcgttc ttctggctat cgtgattgta
    25381 gttaacgtct ttatggcacg ccgactgggc ttcaataagg cagatgaaat tactatcgtc
    25441 ttttgtggtt cgaaaaagag tctggcaaat ggcatcccga tggcaaacat tctgttcccc
    25501 acatcggtga tcggtatgat ggtgctgccc ctgatgattt tccatcagat ccaattgatg
    25561 gtctgtgcgg tgctggcgcg tcgatacaaa cgccagaccg aacagttaca ggcgcagcag
    25621 gaaagcagcg ccgataaagc ttaaagcgga cgcttcaggg gctggaccag ttgcgtcagc
    25681 ccctcggttt taatcagcag cgtgatttgc attagctcac cgagcttccc tgccgggaac
    25741 tcatctttgc gggcaaacca cagcagatac tcctccggca ggtcgattaa gcgacgccct
    25801 ttgtatttgc caaacggcat tatcgtattg gctatttcaa tcagctgctc tttttccatc
    25861 tcagctaccc agcaaacgca gcatttccgc ttcgtcgatg acttcaatgc ccagttcctg
    25921 cgccttcgcc agtttagatc ctgcagcttc acccgctatc accagatcgg ttttcttcga
    25981 cacgctgccc gcgactttcg cgcccagttc gaccagtcga gctttagcgt catcacgcga
    26041 catctggctt aagctgcccg taagcaccac ggttttacca gcaaacgggc tgtcaatctc
    26101 ttccgcgttg ataacgatcg gcgcaggcca gtgaacacct tccgccaaca gctcgctgat
    26161 gacattgcgg ttgctttctt cggcaaagaa gttgtgaacg tgggatgcaa cgacaatgcc
    26221 aacatcaggc accttttgca gctcttcaat cgaagcggct tccagcgctt ccagcgttcc
    26281 gaaatatgcc gccagacctg ctgcggtggc ctcgccgact tcacggatgc caagtgcata
    26341 gaggaagcga gcaaaggtgg tttctttcgc tttttccagc gcgttaacca cgttttgtgc
    26401 cgattttggc cccatacgct ccagtccggt cagtttgcct gcggtgagtt tgaacagatc
    26461 cgccggagtg tggacatatt ctttttcaac cagctgatcg atgattttgt cgcccattcc
    26521 gtcaacatcc atcgcacggc gggaaacaaa gtgtttcagc gactctttac gctgcgcacc
    26581 gcaaatcaga ccaccagtac agcgggcaac cgcttcacct tccacacgct caacgtcaga
    26641 accacatacc ggacaatacg tcgggaatac aacctcacgg gtatcttccg ggcgttcaga
    26701 aagcacgacg ttaaccacct gcgggatcac gtcgccagcg cggcgaatca ccactttatc
    26761 gccaatgcgt aaaccaagac gttcgatttc atccgcattg tgtaaggttg cgttactcac
    26821 cagcacgcct gcaacatgga caggttccag acgcgcaaca ggcgtaatcg cgccagtacg
    26881 cccaacctga aactcgacgt cacgcacaaa ggtcatctgt tcctgcgccg ggaatttaaa
    26941 cgctaccgcc cagcgcgggg ctcgcgcgac aaagccaagc tgctcctgct gttccagtga
    27001 gttgacctta atcaccacgc cgtcgatatc aaagcccagc gtcgggcggt cttcttccac
    27061 tttgtgatag aacgccagca cttcttccgc cgattcacaa agcgttaccc gatcgctgac
    27121 cggcaacccc cactttttaa attgcagtaa acggccaaga tgagtatccg gcagctcgcc
    27181 accttccaga acaccaacgc catagcagaa aaaagtgagc ggtcgcttcg ctgtaatacg
    27241 cggatcaagc tgacgcagtg aaccagctgc cgcattacgt gggttagcaa acactttccc
    27301 gcccgtgcgt cgcgcatctt cgttaatctt ttcgaacccc gcctgcggca ggaacacttc
    27361 accacgcact tccagacgcg ccgggatatt ctctccgtgc aacttcagtg gaatggcgcg
    27421 aatagtacgc acattagacg tgatatcttc cccggtggtg ccatcgccac gagtcgcggc
    27481 actgactaaa acgccatttt catacagaat actgacggca aggccatcca gcttcagctc
    27541 acagcaccag gtgactttct cgttgctttt cagacggtcc tgcacacgtt tgttgaaggc
    27601 aagaaagctt tcttcatcaa aaacgttatc cagcgacagc attggcactt catggcgaat
    27661 ctggctgaaa gccgccagcg gcgcagcacc tacacgttgg gtaggcgaat caggcgtaat
    27721 cagttctgga tgtttggttt ccagctcgcg cagttcgcgc atcagcctgt cgtattcagc
    27781 gtcgggaatt tccggcgcat ccatcacatg ataaagatat tcatgatggc gaagcgtcgt
    27841 tcgcagttct gtcagttgtt gttcgattga ttccatatcg caccatcaat gctaaaaacc
    27901 cccgacaagc gggggttcga agaggagtta atttgcctta agtgtatcag gcgttggcgt
    27961 ctttaacttc gcggatgatg tcctggtact cgcgcaattt ctgcggagtc atcatacggc
    28021 gctgatcgtc aagcacgaca ccgcccactt catcggcaat atgctgcgca gattgcagca
    28081 tcagcttgaa gttctgcagc tcgtcaccgt aagacggtac ctgcataaag atagtgacac
    28141 ccggagtagt gaaatccttc atttcaggat caaaggttcc cggtttcacc atattcgcca
    28201 ggctaaataa cgccgggccg ctgccatccg ggctaagatg acgatggtaa atattcatat
    28261 cgccaaaaat gaagcccgct tgttgaatgc tgttaagaag cagttcaccg tttagctcgc
    28321 taccgtgatg cgccgcgacg ttcatgataa tcaccgcttc tttgcgcttc ggtttatcca
    28381 taactggagc cggttccgct acaggctcag gctgtggtgc cgctacgggt tctgcaggct
    28441 ggaaagcctg ttgtgccggt tgcggtgctg aatgcactgg ctgcggcgct ggcgcgacct
    28501 gtggcgaaac tggctgctgc aacggctgtt caggctgcgg ctgataggca ggctgctgca
    28561 cgggttgctg caccggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag
    28621 gcggctgctg gaccggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt
    28681 gcggcgacgg acgagcagcc tcatgctcct gggcgttagc cggggcatga ttcacgcggt
    28741 gaacacgaac ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt
    28801 cacgttttga cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac
    28861 ggctggtcca gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta
    28921 atatcagacg caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg
    28981 gcaaacattt actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac
    29041 tttcacagca caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa
    29101 ctgtccattg cgcaattacc cggtaaaata cgcacaattt tcctgggatt ggtcaaaagg
    29161 agctcatcct gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt
    29221 ttgcgcaagg ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc
    29281 tgctggtcaa tattttgttg atggggggcg cattctggtg gctctttacg caactcgatg
    29341 tctggatccc gactctcatg agttatgttc cggactggtt acaatggctg agctatctgt
    29401 tgtggcctct ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg
    29461 ctaactggat tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga
    29521 ctggcgctac accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca
    29581 tgaaacgcga atggcaaaaa tttgcctggt atctgccacg cgcaattgta ttgctaattc
    29641 tttacttcat ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg
    29701 cctggatgtt agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta
    29761 aagagatgcg caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa
    29821 ccagtctgtt tacgatgatc ccgctgctta atctgttcat tatgcccgtt gccgtttgtg
    29881 gcgcgacggc gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat
    29941 ctaccggtta ttttgtaaac cgtttgtgtg aaacaggggg ggcttatgcc gccccttatt
    30001 ccatcttgca tgtcattatt tcccttctgc atatagatat gctaaatcct tacttccgca
    30061 tattctctga gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc
    30121 atgagtaaga tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg
    30181 aatcgcatcg gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc
    30241 gttaagtgcc gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa
    30301 ccaggcgttg aactggttga accaaccagc ggtaataccg ggattgcact ggcctatgta
    30361 gctgccgctc gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc
    30421 cgcaagctgc tgaaagcgtt aggtgcaaac ctggtgctga ccgaaggtgc taaaggcatg
    30481 aaaggcgcaa tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg
    30541 ctgcaacaat tcagcaatcc ggcaaaccct gaaattcacg aaaagaccac cggtccggag
    30601 atctgggaag ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt
    30661 acgctgactg gcgtcagccg ctacattaaa ggcaccaaag gtaagaccga tcttatctct
    30721 gtcgccgttg agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt
    30781 aaacctggcc cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat
    30841 ctcaagctgg tcgataaagt cattggcatc accaatgaag aagcgatttc taccgcgcgt
    30901 cgtctgatgg aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgctgcg
    30961 gcgttgaaac tacaagaaga tgaaagcttt accaacaaga atattgtggt tattctaccg
    31021 tcatcgggtg agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa
    31081 ttgcaacagt aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttc aggtgctttt
    31141 ttgtggcctg cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta
    31201 tttaaccaga ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg
    31261 aatcgatttt atgatttggt tcaattcttc ctttagcggc ataatgttta atgacgtacg
    31321 aaacgtcagc ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt
    31381 ctgttaaaaa ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt
    31441 tccacaacac taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta
    31501 ccgctccgaa cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct
    31561 tcacttctga aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta
    31621 aactgcagac tctgggcctg actcaaggta cagttgtgac tatctccgca gaaggcgaag
    31681 acgagcagaa agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg
    31741 ggttctttta aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc
    31801 cccgggtatc gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg
    31861 gaaaaaaatt tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc
    31921 caaggcatca gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga
    31981 aaaagaagcc atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga
    32041 aatcatagcc ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat
    32101 cgaaggtcag gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc
    32161 tgacgtacgt gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga
    32221 cctgagcgcc attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac
    32281 cgcacagctg aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc
    32341 ccacacctct atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt
    32401 cacctctcag gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta
    32461 cgtcaatcca accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc
    32521 tgaaaaagca gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca
    32581 agtagaagta tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg
    32641 cgctgaaggc gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc
    32701 cactgaagaa gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc
    32761 ggttatcgtt cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc
    32821 gaaagaagag aacccgttcc tcggctggcg cgctatccgt atcgcaatgg atcgtaaaga
    32881 gatcctgcgc gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat
    32941 tatgttcccg atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat
    33001 ctacaaacag gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt
    33061 aatggtggaa acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt
    33121 ctttagtatc ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga
    33181 tatgatttca cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt
    33241 tattgatgct tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga
    33301 tgaacgtgct acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat
    33361 ttctatcccg cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt
    33421 agcagagcag gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt
    33481 cattgaagaa aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga
    33541 agatcatggg tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg
    33601 gaactattga gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg
    33661 atgtcgtttt tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca
    33721 aaatggtcgc gcctgtagac ggcaccattg gtaaaatctt tgaaaccaac cacgcattct
    33781 ctatcgaatc tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac
    33841 tgaaaggcga aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata
    33901 ctgtcattga atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg
    33961 ttgttatctc caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtgactg
    34021 tgggtgaaac cccggttatc cgcatcaaga agtaattctt gctgcagtga aaaatggcgc
    34081 ccatcggcgc cattttttta tgcttccgcc agcggcggca aaatcaattc atcgctctca
    34141 tgctgctggg tgtagcgcat cacttccagt acgcgcaacc ccgcgcggtg caccgcatcg
    34201 tttaatgcct tccctttcag caaaccactg ataagctgag cacaaaacag gtcgccagtc
    34261 cctttcaggt cggtttttac ccttgaatgg gaaatgacat tcacgctgtc ggcagtgacc
    34321 accacaacca gcatctcctg attttcttca ttaccggagg cgctggtaat caccacccat
    34381 tttagtgtgt ctgaaagcag actttttgcg gcagcaatgg cactgtcgag atcgcggcaa
    34441 tttttaccgg tcaggatttc caactcaaag atattggggg taattccctg cgccagcggc
    34501 agtaaatatt gtcgatacgc ttcgggaagg tcaggtttga cataaattcc gctgtcaata
    34561 tcgccaatca ccggatcgac catgatcaat aggtcaggat ggtctttgcg tagcgcagtc
    34621 agccactcgg caaggatttt gatttgcgat gccgttccca tatagcccgt ggttacagca
    34681 cgaagttggc gcagcgcatc acgctcctga agtgcacgca aatagccgct aaaccattca
    34741 tccggaatcg caccaccgta gaaagtgtca taatgcggcg tattgctcag caataccgtc
    34801 ggcacggcaa agacattcag gccgttctgt ttgatagcag gcacggcaat gctgttgccc
    34861 acgctgccgt aaaccacctg cgactgcacg gcgacgatat ccgcctgcag cgccctgctc
    34921 ttatcgttaa acaacaacaa actactcatt taattttttc tccttgccga tgatcctcat
    34981 cgtattccaa ccgaaacttt acctgattct ggcagtcaaa tcggctatta caaaacaagg
    35041 ataaggtaat tcagtgaaga aaatcatttg tctggtcatt acactactaa tgacactccc
    35101 cgcttacgcg aagttaactg cccatgaaga agcccgcatc aacgccatgc tggagggatt
    35161 agcacagaaa aaggatttga tatttgtgcg caacggtgat gaacatacct gcgatgaagc
    35221 ggtttctcat ctgcgtctga agctgggcaa tacccgtaac cgcattgaca ctgccgagca
    35281 gtttattgat aaggttgctt cgtcgtcatc gattaccggg aagccgtata ttgtgaagat
    35341 ccccggtaag agcgatgaga atgcacagcc tttttacatg cgttaattgc gcagacggat
    35401 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
    35461 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
    35521 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
    35581 cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc gcgctgatga
    35641 atatccagca cctcatctac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
    35701 cagcggcgaa tgccgggaat gctactgccc tcttccggct gcaggccaac aatggtcacc
    35761 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
    35821 ctggagacaa aataggtaat gcgcccgcca gtctgctgcc agatttccgg cccggtagtg
    35881 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcacca
    35941 cgatccgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgacc
    36001 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
    36061 ggcatcagca atttcatgcg atagccttta agcgcggcaa tcattgccag cgcaatgccg
    36121 gtgttaccac tggtggcttc gattaacaca tcacccggtt taatttcccc gcgcttttcc
    36181 gcctcgacga tcatcgaaag cgccgcacga tcttttaccg aacctgccgg gttattgcct
    36241 tccagtttta accacacttc actgccgtta ttcggcccca ttcgctgcaa cttcaccaga
    36301 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
    36361 ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag
    36421 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
    36481 tgcgagagca agttcctcat ctcggggttc gatacgctcg tcgccgttat acagccgcgc
    36541 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
    36601 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
    36661 tttcgggctg acttccagta cctgtaccgg cagcggcgaa tcgaggctgg tacggcggct
    36721 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg caggtgtgta
    36781 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
    36841 gcggttcact tcgcccataa attcgagcac aaaacgggtc gccggttcgc gccatacctg
    36901 atccggcgcg tcagcctgtt caatattgcc ctggctcatc acaactacac gatcagctac
    36961 ttcggtcgct tcttcctgat cgtgggtcac aaaaacgctg gtgaatttta gttcttcatg
    37021 gagttgacgc agccatcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
    37081 ttcatcaagc agcagaattt gcggttctac agcaagcgca cgcgccagcg ctacacgctg
    37141 tttctggccg ccggaaagct gcgccggata acgatccgcc agatgggcaa gctggaccat
    37201 ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg
    37261 cagcaccgtc aggccaaaag cgatattgtc gaacaccgtc atatggcgga acagcgcgta
    37321 atgctggaac acgaaaccga ctttacgatc acgtgcgtgc aggcggctca cgtcggtgcc
    37381 gtggaagcga atatgcccgc tggtttgatg ctccagccca gcgataatgc gcagcagcgt
    37441 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
    37501 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
    37561 gctcatgatt ttcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
    37621 tactctttaa aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg gcggtaaagg
    37681 agccgactgt gttgtagtcc tgctccagca attcaatctg taacggcagc gaaagtgttt
    37741 cgccacgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatc gcgcgggcgt
    37801 tggtcagcac tacgccataa agcagcgccc agcggatgtt cggcaatgtg acacggcgga
    37861 acatctgcca gccggatgcg ccaagcaaaa tcgccgcttc gtcttcctga ctgccctggc
    37921 ttaacatcac cggcaccagt tcgcgcacca caaacggaca cgtcacgaag atcgtgacca
    37981 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccga
    38041 gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc ggtgacacgg
    38101 caaaaggaat gtccagtagc gtcagcagta actggcgtcc ggggaagtta aagcgcgtca
    38161 ccagccaggc cagcagaata ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
    38221 tcaccgtcag ccagatggcg tgcagcatat ccggatcggc cagattctgt aaaaccggca
    38281 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
    38341 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
    38401 cataacgctt caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg
    38461 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
    38521 aatcgcgctc gccgccgggt aatcaaactc ctgtaagcga acgaaaatca tcagcgacgt
    38581 cacttccgtc ttccaagcga tgttcccggc gataaatatc accgcgccaa actcaccaag
    38641 actacgggta aacgacaacg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
    38701 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
    38761 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
    38821 ggccattgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
    38881 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggcc agcgttaaac cggcgactgc
    38941 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
    39001 ataacgggtt aaaatccacg ccatcagcag gccgaaaacg ccgttaaaaa ttgatgccac
    39061 aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg tgatcacttc
    39121 ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcggaga gtggcagcag
    39181 caatatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcaggac
    39241 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
    39301 ccgccgctgg taaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
    39361 cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc cgggttattc
    39421 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtaaag ccagttcaga
    39481 taggcttttg ctgctttttc ggtaccgttg gcttgcacgt ttttatcgac ccacgccacc
    39541 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcgtac
    39601 tgtttgcgga tgttgttcac ttccgattca aagctaatca gtacatcgcc caggccgcgc
    39661 tcggcaaagg tggtggtcgc gccacgaccg ccggtatcga acacttcaac gtttttcagg
    39721 aactgagtca taaactgttc ggttttggct ttgtcgccac cgtcagcttt atccgctgcg
    39781 ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
    39841 agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
    39901 cggaccagga agcccatggt ggagtagaac ggcgagctgt tattcggcag gcgcgactgc
    39961 cagtccgccg ggataagctt gcctttatcg tgcagaattt gtacgtcggt cacctggtta
    40021 taagtgacaa cgtcggcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
    40081 gcatgagatt gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca
    40141 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
    40201 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
    40261 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
    40321 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
    40381 tgaaaaggga ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca
    40441 gataccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
    40501 ggttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
    40561 atcggcgagg cgacgcagcg ggattgcttt tgccatttca gtcagcaccg actctggatc
    40621 ttccgggttc gactggcggg caatgctttc cgccattggt gtgcgaacgt atccagggca
    40681 tatggcgtta acgcgaatac cagactgcgc atactccacc gccagcgatt ttgtcaggcc
    40741 aacaatcgcc gctttcgtta aggcgtacgc cgtttcaccc ggatcggcca ccatatcacc
    40801 agtgactgaa gacatcatca caatgcgacc atctttgcgg gcaatcatct ccggcagcac
    40861 cgccttcgtg acgttccata cgcctttaat attgatgtca atatggaaat cgcgatcgtc
    40921 atcgctcata tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc
    40981 aatgcgccct tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg
    41041 cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt
    41101 ttcgatctca ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct
    41161 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
    41221 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
    41281 atacaggata aacaaaatat tactgcggat cagctcacta attcaggaaa atctcaatcc
    41341 agtcgctgct ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt
    41401 ttcaatgact ccacatcatt gagttgcaca agaccaacaa acagtaaatc ggtaacagaa
    41461 ttttgcgccg tgcgggtaga catcgacgag ctacgccatt cggtttcacc ggaaacggta
    41521 tcgagcgtga aatgcgccag ccgccgcagc ggagagtcag ccagagaagt aatggcaatc
    41581 accgttgccc cctgctttcg tgccgcttct gcgcacaaga caatctcttt tttactgccg
    41641 ctgtaagaga tagcgatttg tacatcgcct tttttcaacg cctgggaaac ggtggcttgt
    41701 acgtgcgtgt cggcttcgca ggccacgcga tatccaattt tcatcagttt gaacgacaga
    41761 tcgcacccca ccagcgccga tccgcccagg cccgttatct ggataaacgg tgctttactg
    41821 atgacctcaa tgattttttg cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc
    41881 gccagctctt tttcgcgatt caatttgcgg gcaatcactt ccagcgaatc atcactggta
    41941 atcgaactgt gcaggtgctg cgccgtggca ttcgttttct cccggctggc actgtattcg
    42001 cctattaacg ccatacgcaa ttcagtaaac ccttgtgcgc cgagcttctg ggcaaacttc
    42061 acaatactcg actggctgat gccgagctgt ttcgccatct tgcgcgaaga gacggatttc
    42121 agctcactca cgttggcccg cagaaagtca gcaatttttt gctcgttctc ggtaaattca
    42181 ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca
    42241 aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt
    42301 cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa
    42361 atagtaaggt caccaccgat gcaacttgaa aagatgatta ccgaaggctc gaacgccgcc
    42421 tcggctgaaa ttgaccgcgt atcaacgctg gaaatgtgcc ggattatcaa cgatgaagat
    42481 aaaaccgtac cgcttgccgt tgagcgcgta ctgccggata tcgccgcggc aatcgatgtt
    42541 atccacgccc aggtaagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt
    42601 cgtctgggga ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa accgggtctg
    42661 gtagttggat taattgctgg cggcgaatat gccattcagc acgcggtgga aggcgctgaa
    42721 gatagccggg aaggcggtgt taatgatttg aaaaacatcg gattaaccgc gcaggatgtg
    42781 gtggttggca ttgctgccag cggtcgcacg ccgtatgtga ttgccggact ggaatatgca
    42841 cgccagctcg gctgccgtac agtgggaatt tcctgtaatc cggggagcgc cgtttccacc
    42901 accgctgagt ttgctattac gccggtggtt ggggctgaag tggtcaccgg ttcttcgcgg
    42961 atgaaagcag gcacggcgca gaaactggtg ctcaatatgc tttccaccgg gctgatgatt
    43021 aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg
    43081 catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtaatgccga acaagcagaa
    43141 gcagcgttaa ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat
    43201 ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt
    43261 ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa caccattctt
    43321 acccgtgtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt
    43381 ctgggtgtac atgacagttc actggttgat cccgatatca aaacgctgga aggcgtgaag
    43441 ggcgtcattt tgaccagtga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt
    43501 gccgcaaaag cgatgagcga gctgctggga gaagcaccgg tacaggatgc cgcagaaatc
    43561 gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc
    43621 gccaaattcg ccaccatctt cacgccgctt atccccggtt ttattgccgc cggtctgctg
    43681 ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg
    43741 ctacccgacg cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac cttcctggtg
    43801 attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt
    43861 attgccgcac tctttttgct cggttataac cccgctgcaa ccaccggtta ctacgccggt
    43921 tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgttgatt
    43981 gccgcgtggg cctgcgcccg catcgaaggc atggtgcgcc gctttatgcc cgacgatctc
    44041 gacatgctgc tgacctcgtt aatcacccta ctgatcaccg ctacgctggc ctatctgatc
    44101 attatgccgc tgggcggctg gttattcgaa ggtatgtcgt ggctgtttat gcacctgaac
    44161 agtaatccgc tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt ggtatttggc
    44221 gtgcatcagg gctttattcc tgtttacctc gcgttaatgg acagccaagg attcaacagc
    44281 ttatttccca tcctttcgat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac
    44341 tggcgggcgc aaccgcacag tgcattacgc agtcaggtac gcggggcgat tattcccggc
    44401 ctgctgggcg ttggcgaacc gttgatttat ggcgtcaccc tgccccgcat gaaaccgttt
    44461 attaccgcct gtttaggcgg cgcggcgggc ggtttgttta tcggattgat agcctggtgg
    44521 ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg
    44581 atgacttcag cacaaggcat tcttccggcg atggcagtct acgctggcgg gattctggtg
    44641 gcatgggttt gcgggtttat tttcaccacg ctttttggct gccgtaacgt caatctggac
    44701 tgatatcatg aaacggacaa tgctctatct ttctctgctg gctgttagtt gtagcgtcag
    44761 cgccgcaaaa taccctgttc tgacagaaag ttcgccagag aaagcagggt tcaacgtcga
    44821 acggcttaac cagatggatc gctggattag ccagcaagtg gacgccggtt atcccggcgt
    44881 aaacctgctg attataaaag ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa
    44941 aaagtacgat ggcagcgtat tgatggcgca gcctgtaaaa gctaccaccg ggacgctgta
    45001 tgacctggcc tcaaacacca aaatgtatgc cactaacttc gctctgcaaa agctaatgag
    45061 cgaaggcaaa ctgcatcctg acgatctgat tgcgaaatat atttcggggt ttgccgatag
    45121 tccaaatgac accatcaaag gcaaaaacac gctgcggatt tctgacctgc tgcatcacag
    45181 cggcggtttc ccggcggacc cacaatatcc gaataaagcg gtcgcgggcg cgttatattc
    45241 ccaggataaa ggccagacgc tggagatgat caagcgcacg ccgctagagt atcagcccgg
    45301 cagcaagcat atttacagtg atgtcgatta tatgctgctt ggatttatcg tcgagtcggt
    45361 caccggtcag ccgcttgacc gctatgttga agagtcgatt tatcgcccgc tcggcctgac
    45421 acatacggta tttaacccgc tgctgaaagg ctttaaaccg caacaaattg ccgccacgga
    45481 attgaacggc aatacccgcg atggagtgat ccacttcccg aatatccgca cctccactca
    45541 ctggggtcag gtgcacgatg aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc
    45601 aggtttgttt tccaataccg gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg
    45661 gggctatggc gatgtgcagc tgttcagtgc ggaaacggtg aagatgttca ccaccagctc
    45721 taaggaagat gccacttttg gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc
    45781 gacgtttggc acgctggcaa gcccgcaaac ctacggtcac actggctgga ccggaacggt
    45841 gaccgttatc gatccggtga atcatatggc gattgtgatg ttaagcaaca agccacattc
    45901 gccggttgcc gatccgcaaa agaatcccaa tatgttcgaa agcggtcagc tgccgattgc
    45961 aacgtatggt tgggtagttg atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg
    46021 ccagtcagta atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca
    46081 tcaacttgtc cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca
    46141 acatcgcatc acgcttacca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt
    46201 tatgcagacg cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt
    46261 acggcaggct ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg
    46321 tcaggtgaga ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc
    46381 gcccgatcat catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct
    46441 cccaacgctg cacaaacaca tagctgccgc cggcatccac gccgtctttg ataaccgcca
    46501 cttcgcgacg cgtctcttca cccgccgggt tttctgtgcc gtcaacaaag ccgctcaggt
    46561 cacgctcttc cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa
    46621 aggcttccat cgccgcctgg gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt
    46681 ggatcaacac atcgaactgg gtggtcggcg caaggccttt accgtagccc ggaaaatctt
    46741 tcagctcttc tgccccaacg ccgccgctca gagcgcgcca gatgttgtta ccaaaggcaa
    46801 ccaccgcacc aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa
    46861 atgttttact ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa
    46921 tcgccgcgcg gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg
    46981 aaaataataa tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc
    47041 ggcggcggtt tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt
    47101 tttcagggta tcgtccgaag gcattaaccc ttcaggacca ctccattccc cgctgaactg
    47161 gtagctaata tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc
    47221 agctttttgg caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt
    47281 aacaccggtg tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg
    47341 atcgccgtta atcaccatcg ccacgttgtc gcccttcatc acttcaaaaa agcgcaccac
    47401 gttgccgttc gcggttttca ttccgctgcg caggcgataa tcgccatcaa ccgcatcggc
    47461 aatggcttgt tcttgcagtg gtgtggacgc cgttaattca ccaacgccct gctcgctcac
    47521 tttggtggac gacccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat
    47581 cgtggaacag ccggtcagca tcaatggcat agcgcataac attaaacgca gcgatttcat
    47641 gtcacttcct ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg
    47701 ttattctttt acttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa
    47761 cgccacaata tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag
    47821 ccacagccac ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag
    47881 tacacgccag gtgcgaggaa acgtagcccg ccgaccgctc aacaaaaacg ccagcaccgc
    47941 cggaatgcca ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag
    48001 cagcgcagta ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc
    48061 ctgaagcaat agcacaagcc agaagagcaa gggtaaacgc aggcgaccgt gcgcatcata
    48121 atggacagga tgaaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca
    48181 cgtcggcgtg ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg
    48241 gcacgttgat ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa
    48301 gcaacgcatt ggcaatccca cgcccacgaa actctggatg cacgccaaga taataagcag
    48361 acccgcgatg cccgtcataa ccgcccatca ccgttccgac cacttcaccg ttcacctcag
    48421 cgaccagaaa caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt
    48481 tccacggacg cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct
    48541 ggcgaaatac gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta
    48601 tggcgtgaac ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat
    48661 ggggagttgt caactattga aatgaaaagt aaaacaattc tcaacagcaa acggtcgtaa
    48721 cggattacgc gatacgatat aacatctgga actttattat tacaactcag gccgtatgag
    48781 cacttttaaa ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttggc
    48841 tgccctgacg ttgtcaggaa tgtcgcaagc catcgccaaa gacgaacctt taaaaaccag
    48901 caacggacac agcaagccga aagctaaaaa atctggcggc aaacgtgtcg ttgttctcga
    48961 tccaggccac ggcgggattg ataccggagc gatcgggcgc aacggttcga aagaaaaaca
    49021 tgtggtgctg gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc
    49081 gcgtttaacg cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca
    49141 taaacatggc gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc
    49201 tgccggtgct tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata
    49261 cctgtctgaa cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga
    49321 tcatctattg cagcaagtgc tgtttgatct ggtgcaaacc gatactatta aaaacagcct
    49381 gacgctcggc tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa
    49441 caccgaacaa gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga
    49501 aacctcgttt atcaccaacc cggaagaaga gcgactgtta ggcacggcgg cgtttcgtca
    49561 gaaaatcgcc acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca
    49621 gaaagcacat tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt
    49681 tctgctcaac cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt
    49741 tgtcgaagat agctggcagc gcgaaggtgg cggcggcggg cgtagtcggg tgttgcgtaa
    49801 tggtggtgtt ttcgaacagg caggcgtcaa cttttctcac gtccacggcg aggcgatgcc
    49861 tgcttccgcc accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt
    49921 ttctctggta gtgcatccgc ataatccgta tgttcccacc agccacgcga atgtgcgctt
    49981 ttttattgcc gaaaaaccgg gtgcagagcc agtctggtgg tttggcggcg gcttcgattt
    50041 aacccctttc tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct
    50101 gtgcctgcca tttggtaaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt
    50161 ctacctcaaa catcgcaacg aacagcgcgg tattggcggg ctgttctttg atgatctaaa
    50221 cacgccagat ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga
    50281 cgcttattta ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa
    50341 ttttcagctc taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac
    50401 tctgtttggc ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact
    50461 ggtacgctgg gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga
    50521 gtttattaag gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg
    50581 caacgtcaac gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag
    50641 atgccagaat ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct
    50701 tatcagttcg cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt tcgggccaat
    50761 tcctaaaata gcgtgaaacg cgttttgtag cgtgcggcgg ctgacatgca gttgattaca
    50821 caagtccagc accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga
    50881 aagcaaccga cggtaactct gatgactgat gctttccgcc gtcaccattg gctgcgcttc
    50941 ttccagcatc gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg
    51001 ctgatggaga ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca
    51061 cagcgcggct ttatgctgct ctttcacttc cagcgccgac tggctacgca acatatgtaa
    51121 tacccgatcc ggattatgca aaaagttagc ctgacgggtg atgacatctt cagaaagcac
    51181 tacgcccagg atcgtgtaat cgtccggcgt acttaattca aactcggttc caccagggcg
    51241 cgtcgctatt tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt
    51301 cgccggaatg ccaaaccaga acgagttcgg ccagaccaga cacgactgac gcagcgccag
    51361 accggtgtat tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg
    51421 aaacttgccc ggatgcagct gatcgtaaat ctgctgccag gcggtaatcg ttaaagcatg
    51481 ttcatagaca tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag
    51541 cttcaggttt tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt
    51601 tttcatgatg ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg
    51661 cttgagacga taacgactac tgcgtttacg taacgtccca gcagaaaaga gctgttcgag
    51721 cgcatttctg gctttttcca gcggccagcc aaagtgggca gccacttctc ctgccgtcac
    51781 tccctgacgt actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac
    51841 tggcgcgtcg ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca
    51901 ctgggtgtca tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat
    51961 cgcggcagca caacctgcgt ccagcgcagc acgacacgcc gccagatcgc cttccaccac
    52021 cagcgttaag cgacctgggt caagcacttc gtgactgagc agacgaacgt tagctgcttt
    52081 gagcatggca tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag
    52141 tgcattgatc attggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag
    52201 cactgcatcg gtaaaggcgt tacaggccgc tttacacgct gcctggctac cggttaaaaa
    52261 tgctgccgag tagttagttt cagacggtgg cgggacatag gtcaccagct gtacgtcggc
    52321 agatttcaac gctgcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc
    52381 catcggatcg ccaagcgtga tcccggcggt tgatgagaga taagaaccgg tacgcgaaac
    52441 tacatgtgcc aggaatgccg tatcttgcgc atcattagcc cactggaaag cagcgccatt
    52501 ttcaatatgc gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc
    52561 aagcataatc agcacctcac cggcagtcgg tgacgggccg tgtgccgcgc ccgcatacag
    52621 cgagcgacca tataccactt ctaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta
    52681 ggttacgtcg tcagaatcgg cagaaatcag accgaggcta cgaatatgtg gcggcaattt
    52741 cagttcacgc gcaaaatcgg cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg
    52801 tcgaatcaaa tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc
    52861 tttctgctcc agcatacgtt tcgccaaatc cacaatgacg gctgcggctt caaccggcgg
    52921 cgtgccgccc tggtgaatgt tagagataca ggtgcgatcg gcctcaacgg tggtcgccat
    52981 acgcggcgag taaacggcgt agcaggagag gctttctgac tgacccaacc ccggacgttc
    53041 gcccaccagc aggatcacta ctttcgcgcc gaggatctca ccgatctgat cttcaatctt
    53101 cacgcgacca taacgaacaa agaatggcgt gccgactttc agcccggcct gtttcaggcc
    53161 cgccatcagc ggcggcagga tctcttcgta gttcacagtg atggcatcag ttgacaggcc
    53221 atcagaaata acgacctgta cgtccggatt agcaacacac tgtgctttca gcgcttcaac
    53281 agcttctgca cacaggcggc ggcccatatc cgggcgagtc aggtagagat ttttgtcgct
    53341 aatttcggag cgtacttcca gcaagccctg cgctttcacc cactcttccg gcacttcttt
    53401 cagtacggta tctttcgaac gagagtgatc ggccaggaaa cgcagcagcg ccagcgtacg
    53461 cggacgcgga ccggcacgac cggtacaaac gcgggccacg gtgctgcgac gcagttctgt
    53521 taacacatcc gcacgatgtg ggttttcaac accaatccac acttttgctt ccgcagaacc
    53581 taaatccagc gcgcagcttt ccgaggtcac cggtgccgca caggtggtag tggcgcactt
    53641 tgcttctgac ggcgcggggg ccgtttgtcc cattgacgcc atcacgctgc gtacaatttc
    53701 ttcaatctgt ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga
    53761 tcgcccgccc gtttggtcag gcgaccgttt gccataatgc ccatgctttc cagccagcgt
    53821 tcaaactccg gtgacgggcg caggttgagt aactgacgca cagtggcggt atcgtggaat
    53881 gcggtggtct ggtagttgag catgatgtca tcacccagcg gcatccccat gatgtagttg
    53941 cagcctgcgg tggcgagcag gatcatcagg ttttcgttga ggttctggtc agcgtcagcg
    54001 tggttggtgt aacagcagtc acagcccata gagatgccgc tcagcttgcc cataaagtga
    54061 tcttctaagc ccgcacggat aatctggcgg tcgttgtaga gatactccgg cccaataaag
    54121 ccgaccacgg tgttgacgat aaacggatcg taatgacgcg ccagcccata gttacgtgct
    54181 tccatcgtca cctggtctgc gccgaagtta gcgccagcgg atagcgctgg accttgtccg
    54241 gtttcgaagt agaggcagtt ttccccggcg atacggttga actctgcacc cactgcgcgc
    54301 gcttcgtcga gcatcgccag ttccacgcca aactctttca gccctttttc gctgccacag
    54361 atactctgga aaatcagtcc gccaggcgcg ccgcgacgaa tcgcttcgat ctgggtggtg
    54421 acgtgcgcca gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac gccatagatg
    54481 gtgtccagca cgcggcttaa gttttccacg tcgtcagtca ccgggttaac gccgatcacc
    54541 gcatcgccca ccccgaagga aagcccttcg tagatttgcg cggcgatact ttgcacgtcg
    54601 tcacgggtgt catttggctg caaacgggcg ctgaaggtgc ccggaatacc gatggtggta
    54661 ttggcctttt tgattaccgg cattttcttc gcgccgtaga tcaggtccgc gttggagcaa
    54721 atcttcgcta ccgccgcgac cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg
    54781 tccacgctgg tttcatcgct cagcacatac tcacgcagtt cgctgatgct ccagttttta
    54841 atctggttgt aggccgtttc gttaacatcg tcctgaatca gccgcgtcac gcagtcatct
    54901 tcataggcaa tcaccggatt attgcggatg tccgctacgg tcatttccga caacacctgc
    54961 tttgccgcca cgcgctcctg tgagcttgcc gctgcgacgc ccgccagcac atcccccgaa
    55021 cgcagttcgt tggctttagc cagcacctct tttacatcct taaactgata tacattgccg
    55081 aacaatgtgg tctttagttt cataagtcgt tccctcagga aggaaatgcg agtgatttca
    55141 ccgtcaccgg cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg
    55201 cgcggacaat cacttcgtca atgactgcca acgggagttg ttgtagctgt gggcgcaaca
    55261 gcatgcccag agctttacca aagtcctgcc cggccaccac cagcagggga tgcggattcg
    55321 gaaaacgcgc gacgaaatcg accaacgcgt tgatgaccgt cagtaccgcg gcgtaacgca
    55381 caggcagcga ggcgggaagc gccagcacgt acgcgtcagt tttgggatca agatccagct
    55441 gaatcagcgc ctgttgccag gcactcacca gatccgtttc atcaatcggg atcgccaccg
    55501 gcaaattgcg cagcggcagt tgtacgccct ccagccagat tgtgctgcca gagagcgaaa
    55561 gggtatgtgc gcccgcgcca atcaccgtgg cgcgcacggt ttgcgccgga aactgcacgt
    55621 tcatctcacg caggcgcgga tggtcatgta gcgccgtcgc cagtagcggg ccaatatcgg
    55681 caaaacagaa cgggtcagcg ggctggtggc gataacattc gcccacgccg ccagaaagcg
    55741 taatgatttc gggcgtaaca cctgcgggca gcaaaccggt ttgcattaat gcctgcgcga
    55801 gcggcgaaag cgttccgtca atcacttcga caatcagctc tgccatccgt cgggtaacct
    55861 gcaccagctg cgcgccggtc agcgaacgga cgtcagtgcc tgcaccgaaa cactcatcca
    55921 caatcatctg ccccggttta tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca
    55981 ggcgaccacc gacgttgagg caggcagtgc cgctgatttt tccggcatcg aacagggcgt
    56041 agttcgcggt gccaccgccg atgtcgatat tcagtacccg acacagccgt tgttcagaaa
    56101 gggtttgcgc cccggcaccg tgaccggcga tcacggattc gaggtgcggc ccggcgctgg
    56161 cgacgacaaa atcgcccagc gactgagaga gcgccatcac cgccgggcga gcattgcggg
    56221 ttttcgcgct ttcaccggtg atgatgatgg caccagaatc aacgctttcc ggcgcaatgc
    56281 ccgcagcctg atattgctct aaaatcagcg atttcagttc cgcttctttt aaaccaccct
    56341 gtttatcgac aggggtaaag aacaccgggc tttgccagct aatttcgcgt ttgatgaatt
    56401 cgtagcgcgg cacctgcgac accgccgcac ggttaaccag ttccaggtgg gagaaaatca
    56461 cctgggtggt ggtggtgccg atatcgatac cgacgctcag tagctggcga gtgttcacga
    56521 ttgtgcctcc gcttcggttt tagccgcggt cgcgtcgtct tttggcacca gcatcatcgc
    56581 cacgccaatc gccgttacgc cgccgatcaa cttgccgaca atcatcggga agatcatggc
    56641 attcatgttg gcagcggcga agcctaagtg gtcgcccagg gcgaaagcag cggaaacggc
    56701 gaaggcgcag ttgatgactt tgccgcgggt atccatctgc ttcatcatgc cgaacatcgg
    56761 gatgttgttg gcaagcgttg ccaccatgcc ggctgccgcg atgttgttca tattcagcac
    56821 tttaccgacg ctcatcagcg gtttttcaaa ccagcgggtc agcagcagca ccatcggata
    56881 cgcccctaac agaacgcagg agatagaacc gataacttca atggcgcgca tcacctcacc
    56941 gggtttatcg ccaggggcca taaagatagg atccagaccg gggatcagtt cccagccaag
    57001 caggaatttc actaccgcag cggcaagacc gagggtgatc aatgcaacga ggaatttggc
    57061 gaagatctgg aagccgttga tcattttttc tgggatgaat ttcagcccca gcgccaccag
    57121 aatcgcaaca atgatcaccg ggatcatgtt catcaggatc agggcgaaag tgaattccac
    57181 cggctggccg ttgatctgaa caccggagta catagcaacc agaccaccag cgatacaacc
    57241 aatcggaatg gtcacaatgc ccgccagcac gccgagcgcc agataacgac ggtcagaagg
    57301 ttcgataatg ccgagcgcca ccggaatgga aaacacaatc gttggcccca tcatcgaccc
    57361 gagaattaac ccagagtata gccacgcggc tacgtcgccg cccgccagct ctttggcgag
    57421 gaagaagccg cccatatcgc acgccagcag tgttccggcg aacatcgatg ggttagcgcc
    57481 gagcatttcg taaaccggaa taattaccgg cccgagtacg tgagccagta ccggtgccag
    57541 cgcggtcata ccgaccatcg ccaggcccag tgcgcccatt gccataaagc cttcttcgaa
    57601 ctgaccgccg gatccttcga tacttttacc gaacttaccg aggaaacgag cagaaccgcc
    57661 gaactgtgac aggatcctgt caacggcagc tatcagcata aagaacatca tgatgtacat
    57721 gatgatttcg ttaattccca tcgcctttac tccctgttag ttgttattta ttagcggatg
    57781 ctgcgtgaag ccttatccgc actacatgtg caatcccaca gaccggataa gacgcggcat
    57841 ttattgcgcc gctgcgtaca ggccgacaag ctgttccagg ctggcggtac gcgggttgct
    57901 gcgcagacaa atatcttcca gcgcggcctg cgcccatgca ccgtaatgcg cagatgtcgc
    57961 gccaacatcg cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt
    58021 aatagcgtca cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg
    58081 ttcgcgacaa accatccggt taaactccat caccgttggc agcaacatag cgttcgcgag
    58141 accgtgcgga atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag
    58201 acccgcactg gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc
    58261 ggcaaggtcg tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc
    58321 accaatcgcc agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc
    58381 atgggttaac gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa
    58441 tgcggcgtcg aggatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg
    58501 cccgctcacc gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt
    58561 agttggaatg gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc
    58621 cagcgtgcta tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga
    58681 gccgccgcca aatgcgatca ccccgtcaca acctgactca cgcaactgcg ccacggctgc
    58741 acacacgtcg gtaatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatacc
    58801 tttaacggcc aggctacgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc
    58861 tgccatcacg aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac
    58921 cgagcccgga ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt
    58981 cagggtatcg aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg
    59041 ctttctctcg cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta
    59101 aatgcacctg taacgccggg aattgtttgc gaaacagttc tgccacgccc ggttgcatac
    59161 aggagccgcc cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa
    59221 tgtccgccat tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc
    59281 gcttgtactg ctccgcctct tccagcgaaa tacggcgatt tccggcgaaa gtcagagaga
    59341 tgtgatgtcc gccggtggct tcatccgccg agtacgtcac tttgcccttt ttcacgatgg
    59401 caatgccggt agtgccaccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat
    59461 ccgcgactgc ggttggctca tccagcacat ggctcacttc cagcccggca gactccagca
    59521 cgttgatgga aatacgcggg tccgtacctg gaggaaatga agtcgccaca tggctaaaac
    59581 gacgaccgaa ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg accgcgccga
    59641 agaaatccca gacgatgccg tcgcgaacca catcggccca gtcgaggcat accgccactg
    59701 gctgaccgtc gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccactccca
    59761 gccacagcgg agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca
    59821 gacgtggggt gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac
    59881 gcatcgacta atacacagcg acgcagacgg acaaacgtac gcgcgctggt caccccttca
    59941 ccggttggcg tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca
    60001 atgcacggtc cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg
    60061 ttttcgatgt tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc
    60121 gctagcgcaa tggcatccgc cacgttggcg acgcgcacga cgggcaacac cggcatcatc
    60181 agttcagtca cggcaaacgg atgttctgcg gtggtttcca caaacagcag gcgcgtttct
    60241 tgcggaactt taaggccgat tgccgccgcg attttgcctg cgtcgcgacc aacccagtca
    60301 cggctgacgg tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc
    60361 tgcgcctgtt ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca
    60421 tcggctacgc tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg
    60481 aaagacgcgc ctttgacgat ggactgagca gcacgggcga ggtcagcggt ttcatccacc
    60541 actaccggcg gattgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc
    60601 gcttctacta ccgcttcgcc gccggtcacc accagcaggc cgatacccgg aaacttgaac
    60661 aagcgttgcg cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg
    60721 cctgcggcaa caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc
    60781 gccggatgcg gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta
    60841 attacggttg ccgccgggtt agtggacggc gtcaccgaag ccaccacgcc ccagggcgcg
    60901 ttttcaatta aagtcaggcc gttgtcgccc gtcagcactt gcggagagag acactcaacg
    60961 cctggcgtgc cgcgtgcctg agcgacgttt ttggcaaatt tatcttcaac gcgacccatg
    61021 ccggtttcac tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcttcacga
    61081 atggcagcaa tggctaactg gcgcattgcc acgctcttta acccttgctg ggcaattttg
    61141 gctgccgcaa cagcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc
    61201 ggcgtgtcac tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt
    61261 tgattcatga tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat
    61321 cgacgatgcc aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg
    61381 cggaactgcc actcaccagc aacacccact ccccggttcc cgcgccaata ttgtcgatgg
    61441 cgacggcaca ttgcccgtca ggattaccat gtggatcaat catttccacc atcagcaatt
    61501 tgtcatgcgc cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca
    61561 gtttcatacc cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcggaag
    61621 gtaaaatgaa aaatgagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg
    61681 ttgctgctat cgcctttcag gccgatcggg aacacttctt ccaggtcgcc gtgcggacgc
    61741 gggataacgt gtacagatac cagctcgccg atacgctgtg ccgctgcggc accggcatcg
    61801 gtcgccgctt tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca
    61861 atctgcttaa caccaaccag cttgacgcgc gcagctttca ccatcgcgtc agaagcctca
    61921 atcagtgcaa ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct
    61981 ttatcagggt ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt
    62041 tcacttctgt ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacac
    62101 tacaaccacg agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg
    62161 cacgatatcc gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg
    62221 gaaaaaccat cacattggcc ttgccctgta gcgggctggc aggcgctttt tgcgccgcca
    62281 cttccggcac gaaggcggcg tcaaactgca actcgccatc gactaccagc tttggtgcgc
    62341 gctcacggac gatttctgtc gcctgctgga cattggcaac gcaggggtga cgggcgctac
    62401 cgttactgga aaacgacagc atcgccacgc gcggctcttc tccggtgatg gcgcgccagg
    62461 tttcggcgct ggcaagcgcg atatccgcca gctgcgccgc cgtcggctgt ggtaccacgc
    62521 tgcaatcggc aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg
    62581 agagcgtttt acagcctggc tgcaagccga taatgcgtaa tccggcacgc agcacattcg
    62641 ccgtggaaga gaggttgccc gcgatacaga catccgcttt accggcgctg accattgcgg
    62701 cggcgaacat cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg
    62761 cgcgggccag ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcta
    62821 tcacctgtag cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg
    62881 gattggcgac cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca
    62941 gcacacgttg atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt
    63001 cacaacaacg ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt
    63061 tagcggctgc ttcgttacgc tcaggatcat catcacgtag accgtgctgg aaaggcggtt
    63121 cagcgcctgc aagatgtccg ggcgtaatac ttcaaaactg cgggtgataa acacctgcgc
    63181 cgctacggtt tcggcttccc gcactttggt gcgtagcaga ttcagcaacg ccgcatcgcg
    63241 cccgtggctt gcttccggca ccagatgatc gtgaccaaga taacgcaacg gctgatgaga
    63301 aagccggtgc agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttccgc
    63361 cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaactg cgcgaaaacc
    63421 cagacgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca gcgtatccgg
    63481 cttttttgcc accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact
    63541 ggttagccca tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg
    63601 ctcgtcaata aacttgatgc gcagatggcg gctttccagc aactcccggg cagagggcgt
    63661 caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct
    63721 tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg ccaggcgaca
    63781 tacaggaatt tcacgctgga tgtcgtacca aattcgatgc tggagccttt cgggataaac
    63841 atcacgtcgc ctgctttagc gatcatggtt tcgccttcgt ggcgaacatg gagctcccct
    63901 tccagcacca tgtcgatttc gtcgtagttc agggtccacg ggaagaatgc gttctcccac
    63961 tgcataaaac cggcggccat gctgctgccg tcgtctccgg tcacaagatc agtcaagccc
    64021 acgcaatgcg gttgtgcgcc atcaaagcga ccaaacttga cgctgctacc atcaatcact
    64081 ttgatgccgc ctttgccggt caccgatttg aagctcggct gcattgcacc ctgctccagc
    64141 gactgttttt ccttcatcac tttttccatc agctgcgcga ccaggctttc ggtaaactgg
    64201 ccttccggta gctgggcgat aatggtttcg cggatgcgct ggctttcggt tttatcggct
    64261 ggcacgctgg cgggcacgga cgccgttacc gggatcaatt catcacattc ggtgatggta
    64321 aaacccagta gatccgccac ttcacgggct tccggggtaa taatgctggc gcgcagaacc
    64381 acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt
    64441 tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac
    64501 gctttgcggg tcgtggctgt ttagctcgaa aatcggctcc tcgaagcccg tctcaagcaa
    64561 taactttcgc gttgcggcga cgtcagcatc cggcatgtcc gttttactga tgacggcgat
    64621 ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt ccgggtcatt
    64681 cgcgccgtgg acatagatca gcatgtccac atcctgtagc gtggtaatta aggcgtgata
    64741 ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgcccttat cattaaattc
    64801 cacggcctgt gtttttctgg cgagggtata attcccctgc aacgcattaa ataacgttgt
    64861 tttccctgcc ccgacggtac ccacaaacgc aattcgtttc atgaatgcct cgtattaact
    64921 tttggtcatt tcacaaagcg tataatttaa taagcgacct aaaccactga cggtttgtga
    64981 taacgcttct tctactgcgc ccaccgagcc ataaatcacc aatgcaccac tgaagcggtc
    65041 gagaaatccg atatgcacat cagcggcttt cagcgctaaa tcaccggcga tcatcgccgt
    65101 ttcgccggga gttagcgtca taatgccaat tgcgcccgca tccggaacgc cgattttctt
    65161 cgccagttcc tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt tgccgggcac
    65221 aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta
    65281 tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt aaaaggtgag
    65341 gtagatcact aagaatggag agatatttac tgttatgaat aaactttcat atgcgacgca
    65401 tgaatataaa taaaataata tcgagatatt ttatatactg ctttttttat tattcgcaaa
    65461 ctcagtaata agctttacta atatattgca gctaaaccgc ttaccctgaa tattcagggt
    65521 aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg
    65581 ccaccatgtt gacgatacga cgcaccgatg cgatcggcgt taacacgtga accggtttcg
    65641 ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg cgcagtaagt
    65701 tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattggca gaacctttca
    65761 aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca
    65821 tttcaccatc aatcatcagt tctggcgcac gttccctgac cagttccagc gcctgacgca
    65881 ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagcaacgc
    65941 gcggttcaat accgaaacga cggacagttt ctgccgccat caaggtgatc tccgccagct
    66001 cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta ccactcggca
    66061 gcagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt
    66121 tcaccacgct aaaatgttca tgataatcac ccaccgtacc gcaaatcatt gcatcggctt
    66181 cgccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcccgctgcg
    66241 cctgttcctg agtgacgccg cgacgcttca tgatctggaa gtattcggtc cagtactctt
    66301 taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgct ttgatctgca
    66361 agcccagttt ctgaatgcgc atttcgatca cgttcggacg accgataagg atcggtttcg
    66421 ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct tccccttccg
    66481 gcagaactac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt
    66541 tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgcaatca gcaatcggac
    66601 gcgtcgccac gcccgactcc atcgccgctt tcgcgaccgc aggagcaatc ttgactatca
    66661 gacgcggatc aaacggtttt ggaatgatgt attcagggcc aaagctcaga tcctgatcgc
    66721 catacgctga agccaccact tcactctgtt ccgcatgggc cagttctgca atcgcgcgta
    66781 ccgccgccag tttcatctct tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga
    66841 agatgaacgg gaagcaaagg acgttgttca cctggttcgg gtagtcggaa cggccggtac
    66901 agatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccgggt
    66961 tcgccagcgc caggatcatt ggcgctcgag ccattttctt caccatttcc tgggtcagca
    67021 ctttcgggcc ggaacagccg aggaaaatat ctgcgccttc aatcacatca tcgagggtac
    67081 gtttgccgtc atccactacc gcatatgcgg ctttggtttc cgccatgttt ggctcacggc
    67141 cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccaacg
    67201 ctaccagcag gttcatacag gcgattgctg cagcacccgc gccggaaacc accatacgca
    67261 cgtcagagat gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgataa
    67321 ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct
    67381 gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgatgccg ccgaaggtcg
    67441 gttcgagcgc agcgacgact tcaataaatt tgtccgggtc gagttcatca acttcaatgt
    67501 caaatacatc aatcccagcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt
    67561 tgcctgccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg
    67621 ccaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa
    67681 gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc gttgccagag
    67741 gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac
    67801 tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaagtg
    67861 tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccttgccg gagtctacag
    67921 actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa tcctataaca
    67981 ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt tttgatgcgt
    68041 ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta cttttcaagg
    68101 agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg tggcagacag
    68161 cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca atccttcgct
    68221 gttactcaag gctgccggat tatcacaata tgagcattta atagacgatg ctatcgcctg
    68281 gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg acaaactggc
    68341 ggtcaatttc ggtgctgaaa tcctcaaaat cgtacccggt cgcgtgtcaa cagaagttga
    68401 tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggactt
    68461 gtatcagcaa caaggcgttg agaaatcacg cattctgatc aaactggctt cgacctggga
    68521 aggaattcgc gcggcagaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct
    68581 gttttctttt gcacaggcgc gggcctgtgc agaagcgggc gtttttctga tttcgccgtt
    68641 tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggacccat atgtggtgga
    68701 agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc aacaccacta
    68761 tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg
    68821 ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa aagtttcgcc
    68881 agtggtacgt aaattaatcc caccttctca gacgttccca cgcccagctc ccatgagcga
    68941 agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg
    69001 cattcgtctg ttcgccgttg atcaacgcaa actggaagat cttcttgccg ccaaactata
    69061 aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca
    69121 gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg
    69181 atattgccga agtgctgtgg aacgattttc ttaaacataa ccctaccgac ccaacctggt
    69241 atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc
    69301 tgcatctgac cggttacgac ctgccgctgg aagagctgaa gaactttcgt caactgcatt
    69361 cgaaaacccc tggccacccg gaaatcggct atacgcccgg agttgaaacc accaccggtc
    69421 ctcttggaca aggtttggcg aacgccgtcg ggctggcgat agcggaacgt acactggcgg
    69481 cgcagtttaa ccagccggat catgaaattg tcgatcactt cacctatgtg tttatgggcg
    69541 acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcgggc acgctgggac
    69601 tgggcaagct gattggtttt tacgatcaca acggtatttc gattgatgga gaaaccgaag
    69661 gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat gtgatccatg
    69721 aaatcgacgg tcacgatccg caggcggtga aggaagcaat ccttgaagcg caaagcgtga
    69781 aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag
    69841 caggtaagga agaggcgcac ggcgcaccgc tgggggaaga agaagtggcg ctggcacggc
    69901 aaaaactggg ctggcaccat ccgccatttg agatccctaa agatatttat cacgcttggg
    69961 atgcccgcga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt gccgcctata
    70021 aaaaggctca tccgcaactg gcagaagagt ttacccgtcg gatgagcggt ggtttaccga
    70081 aggactggga gaaaacgact cagaaatata tcaatgagtt gcaggcgaat ccggcgaaaa
    70141 tcgctacccg taaggcttcg caaaatacgc ttaacgctta cgggccgatg ctaccggagc
    70201 tgctcggcgg ttcggcggat ctggctccca gcaacctgac catctggaaa ggttctgttt
    70261 cgctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggcatga
    70321 ccgctatcgc caacggcatt gcgcaccacg gcggctttgt gccgtatacc gcaacgttcc
    70381 tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc
    70441 agattatggt ttatacccac gactcaattg gtctgggcga agatggtccg acgcaccagg
    70501 ctgttgagca actggccagc ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg
    70561 atcaggtgga agcggcggtg ggctggaagc tggcggttga gcgccacaac ggaccgacgg
    70621 cactgattct ctccaggcag aatctggccc aggtggaacg tacgccggat caggttaaag
    70681 agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat attattttga
    70741 ttgccaccgg ttcagagatg gaaatcaccc tgcaagcggc ggagaaatta gcgggagaag
    70801 gtcgcaatgt tcgcgtggtt tccctgccct cgaccgatat tttcgacgcc caggatgagg
    70861 aatatcggga gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg gaagcaggta
    70921 ttgccgatta ctggtacaag tatgttggtc tgaaaggggc aattgtcggg atgacgggtt
    70981 atggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata
    71041 ttgtggcaaa agcgcataag gtgctgggag taaaaggtgc ctgatggtga tcgccggatg
    71101 ctgattgccg gatgcgacgc tgacgcgtct tatccggcct acagcgtctt atccggccta
    71161 catgtcccgc cattttgttt aacgggtgat ccacaacgtg ggccaggcgt ctggcccatg
    71221 ccagttatcg caggtgggtt ctgcggcgta acgcaccagg cgaaaacgct gaccgtcaaa
    71281 gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc cctttcgcta aggtcaccag
    71341 ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc agttcattcg tctcctgacc
    71401 gttgttgaac ggcaaacgca gccggaccgg gcgcgaggcc agcggctttt tacgcgacac
    71461 aatccatgcc aaatcaatgg tgttataggc ccctgcctca cagctaatca tcatcagcgc
    71521 tttatcatca gtcagcgcag tgacattcac ctcacgacgc aatggatcaa gcgagcagcg
    71581 cagaccattc atccgccagt ttccataatc cagcaaatcg ttgcgttctt cgagtgagag
    71641 tggcgtcggc gttgggttaa ccaccacgac ctctttcagc gcaggcgcgg gcggtacgct
    71701 gagcggcggt tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc gcttttgctg
    71761 agcatcaata aacaacaacg ccgcttttaa gtcactcaga gaaatggtct gattgccgtc
    71821 gcgtaaggtg atcgccttcc cttcctgaat catctgcaaa aacgcggtga tggttgccgt
    71881 atcatcggtt actaataacc atggtgaaat ccgccacttg tcaccactta acgccaacgg
    71941 ctcgccatct aacagcagcc gtggcgcaat ttccccttca gacgcatccg gcgacttcaa
    72001 cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc ccggcgctgc ggctcagggt
    72061 catcaccagt ccattatgat cgcccgtatt acgcgccacg cagaagtttt ggttattaca
    72121 ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc gcccacacca aagacgtcgg
    72181 caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta cgaccccaga
    72241 agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat
    72301 ttgtcggatt gagccaagaa taaacaacga aatattattg ttatttttca gtctattaaa
    72361 tgtgacattt gcaaatagtt aagcaataac accgtcttac cgtcacgtat ctcgccggtt
    72421 ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc ttcatcttcg
    72481 acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa aaagtggatt
    72541 agctcagtca caccgcctgg cgacatatac agttcaaata atttgcgcac ttcgcccact
    72601 tcatagcccg tctcttcaat cgcttctttg cgaatgcaca cttccggttc gtcgttatcc
    72661 agcagcccgg cgcaggtttc aatcagctgc ccgctttcat tgccattaac ccaggtagcg
    72721 acacggaact gacgaatcag aaccacggtc tttttcttcg cgttgtacag gaggatcgtc
    72781 gcaccattac cgcgatcgta aacttcacgt ttatggcgga taacttcgcc atctttgcgc
    72841 gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat tttgtcttta
    72901 atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa ttatattacg
    72961 ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt ttacccccag
    73021 ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa ccaccagatc
    73081 cgcaccacgc acggcgtcgc caccggcgaa gattttcgga ttggtggtct ggtaacggta
    73141 ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac cgtgcgactc
    73201 cagccacggc atcccgtgcg gattgaagcc aaacgccata atcaccgcgt cggctggcat
    73261 gacaaattca ctgccttcca ccggcactgg acgccgacgc ccctgggcat ccggctcgcc
    73321 aagacgcgtg cgcaggaaac gaatcccgca gacgtgacct tgttcattca gctcaagcgc
    73381 caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttct tcacctcttt
    73441 cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt tactcgcgcc
    73501 gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg ccgccgccca gtaccacgac
    73561 gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc ccatcacctg
    73621 tttagtgttg gcgatgagga acggcagcgc gtcataaacg cccggcgcat cttcattggg
    73681 taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc
    73741 caaaagcgaa tccaaagaga catctttacc cacttcacaa ttgagttcga agtgaatccc
    73801 catcgcgctg aagatttccc gacgacgtgc cagcagggat ttatccagtt tgaaagaagg
    73861 aatgccgaaa gtgagcaagc caccgatttc tggatggcga tcgtacaccg tcaccgccac
    73921 gccattgcgg atcagaacat ccgcacaggc cagccctgcc ggacctgcac cgataatcgc
    73981 cacccgcttg tccactttgg tgacatggct taagtcagga cgccaacctt tcgccaacgc
    74041 ctgatctgaa atgtagcgtt caatgttgcc gatagttacc gcaccgtgct catcgcgaat
    74101 agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa tttccggcag
    74161 ggtgttggtc aggtgagaaa gctcgacggc ggcgtcgatg tttccggctt tcaccagttc
    74221 aatccactgc ggtatatggt tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc
    74281 gcatttaagg cagcgcgagg cttcccgttg tgcctgatcg gcacggaatg gcagataaat
    74341 ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta tccggctcgc cacgcgcggg
    74401 cgttgcctgc atttgttcga ctttactcat taccggcatt tcttgtgcag cggtactggc
    74461 atgccacggt tgatgttcct gacgtgcggt acgcaagcgg cgggatttcg ccatgcctga
    74521 gagtgcggcg tcagtgacca gctgcagcgc gtccgccggg caattctcga cacaggcagg
    74581 accgttttcg cgccctgcac aaaggtcgca tttatgcgcc gtggctttaa ctttgcccgc
    74641 tgcgacgggt gtcaggacga tttgcatcgt accaaacgga caggccacca cgcaggattt
    74701 acagccaata cacttttgct gattgacctg aatgctgtca tcaacgtggc tgattgcgcc
    74761 attagggcag ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca ctgcactacg
    74821 ttgctgctga tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg
    74881 ttgctcatca ttgtgagcca tgacacaagc gatttcacaa gcatgacaac ccagacattg
    74941 ctgactgttg gccataataa aacgattcat aacgaccttc ttttttggtt gtaaaaacct
    75001 tattctttat atgagtgttg ttattacccg acttacaggg gaatcggcaa tgttcatgtg
    75061 cccagaataa gtaactattt cgctataaac tgtggcagat caaataatcc catcactaac
    75121 taaattgcgt ttcagcgaac tggaacatta atgatttttt gtggagaaga cgcgtgtgat
    75181 tgttaaacga cccgtctcgg ccagtctggc ccgggccttt ttttacattg tgctgctgtc
    75241 gattctttcc acgggtatcg ctctgctaac tctggcgagc agtttgcgcg acgctgaggc
    75301 tatcaatatt gccggatcgc tgcgtatgca gagttaccgc ctgggctacg acttgcaaag
    75361 tggcactcca caactcaatg cacatcgcca gctgtttcag caggcactgc attcaccggt
    75421 attaaccaac ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct atgcgcatct
    75481 gaatgccaac tggctggaga tgaataatcg gctcagcaag ggcgatttgc cgtggtatca
    75541 ggccaatatt aataattatg ttaatcagat agacctgttc gtgctggctt tacagcacta
    75601 cgctgaacgc aaaatgctgc tggtggtggc gatttccctg gctggcggta tcggtatttt
    75661 cacgctggtc ttttttactt tgggccgcat acgccatcag gtggttgccc cgctgaatca
    75721 gctggttacc gccagtcagc gtattgaaca cggacagttc gactcgccac cgctggatac
    75781 cagcctgccg aatgagcttg gtctacttgc aaaaaccttt aaccagatgt cgagcgagct
    75841 gcataaattg taccgttcgc tggaggcgtc agtagaagaa aagacccgcg atctccacga
    75901 ggccaagcgt cgtctggagg tgttgtatca atgttcgcag gcgctgaaca ccagccagat
    75961 tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag cggctgaata
    76021 tctggagtta aatgtcggtg aaaactggcg gattagcgaa gggcaaccaa acccggaatt
    76081 gccgatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac tgcactggca
    76141 aaatagtcac gtttcatcat cagaaccgct gcttaacagc gtttcgtcga tgctgggacg
    76201 cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga tggaagaacg
    76261 tgcaaccatc gcccgcgaat tgcacgactc gctggctcag gtactttctt acttacgtat
    76321 ccagttgacg ttacttaagc gttcgatacc ggaagataac gccaccgcac aaagtatcat
    76381 ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc tgttgactac
    76441 ttttcgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa tgctggatac
    76501 gttacaaaat caaaccagcg ccaaactgac cctcgactgc cgtctgccaa ccctggcact
    76561 ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc tgaatgcgat
    76621 gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg acggcaatca
    76681 cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca
    76741 ttatggtctg aatatcatgc gcgaacgcgc ggaacggcta ggtgggacgc tgactttttc
    76801 acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctctgcgg agggtgagga
    76861 aagtcagttg atgtaatgcc tcctactgac caaagaatac ttgcagttaa ggttcagtat
    76921 aaaagggcat gataatttac attaactcct ttttttctcc acgattggct cgtaccttgc
    76981 cgctacagtg aagcaagtca agcctacaac gatacgcaga aacacgaggt cctcttttaa
    77041 tggcgaattt ctttattgat cgccccattt ttgcctgggt gctggcaatc ctgttgtgtc
    77101 tgacaggtac cctggcgatt ttttcattgc ccgttgaaca ataccccgat ctcgcgccac
    77161 cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg
    77221 tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctcatgtat atgtcatctc
    77281 agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg
    77341 acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg
    77401 cggtgcaaaa tcagggcgtg actgtgcgta aaaccggcga taccaacatt ctgaccattg
    77461 ccttcgtctc taccgatggt tcgatggata aacaggatat tgctgattat gttgccagta
    77521 atattcagga cccgttaagc cgcgtaaatg gcgtcgggga tatcgatgcc tatggttcgc
    77581 aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacagcta
    77641 aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg
    77701 gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc
    77761 aaacaccaga acagttccgc gatatcacct tgcgggtgaa tcaggacggc tcagaagtaa
    77821 ggctcggcga tgtcgccacc gccgaaatgg gggcggagaa atacgattat cttagccgct
    77881 tcaatggtaa gccagcctcc gggttggggg taaaactggc ctccggcgcg aacgaaatgg
    77941 cgacagcgga gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcacggac
    78001 tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg
    78061 tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa
    78121 acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct
    78181 ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg
    78241 cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga
    78301 gtgaggaagg actcactcct cgcgaagcca cgcgtaaatc gatggggcag atccaggggg
    78361 cactggtcgg gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg
    78421 gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt
    78481 cagtactggt agcgatgatc ctcactccgg ctctgtgtgc cacactactt aagccactga
    78541 aaaaaggtga gcatcacggg caaaaaggct tttttgcctg gtttaaccag atgtttaacc
    78601 gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga
    78661 ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt
    78721 cgttcttacc gctggaagac cgtggcatgt ttactacctc ggtacaattg cccagcggtt
    78781 caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcacccatg
    78841 aaaaagacaa catcatgtcg gtgtttgcca ctgttggttc tggccctggg ggtaacgggc
    78901 aaaacgtggc gcgaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg
    78961 gcacctcgtt tgccattatc gagcgtgcaa caaaggcgtt taaccaaatt aaagaagctc
    79021 gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg tagttctgca ggttttgata
    79081 tggagttgca ggaccacgct ggagcaggtc acgatgcgct gatggcagca cgtaaccagt
    79141 tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac ggcctcgatg
    79201 acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgcta
    79261 tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtg aatgacttta
    79321 tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc
    79381 cagatgacat caatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg
    79441 ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct ggaacgctat aacggttatt
    79501 ctgcggttga aattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata
    79561 ttatggaatc gttagtgaag cagctaccaa acggctttgg tctggagtgg acggcgatgt
    79621 cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt tccttgctgg
    79681 tggtattcct gtgtctggct gcattgtatg aaagctggtc ggtaccgttc tcggtaatgc
    79741 tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa
    79801 acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca
    79861 tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccacgacctg tttgaagcga
    79921 cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct
    79981 tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg
    80041 gtactggcgt aatgggcggg atgatttcag ccactattct ggctatttac ttcgtgccgc
    80101 tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa
    80161 aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac ctgctcagcg
    80221 ggttcttgct gtttgtactt tgtctcagga attacttacg aagcataact tcaataaagt
    80281 ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc
    80341 tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg
    80401 ggggacaaac cttatgtaaa tactatggtc ctgcagtgat gatttgtatg tgatacacag
    80461 caacatttcg agatattcat acggcatcta atactgattt aa
//
LOCUS       DAZGXS010000006        79336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGXS010000006 DAZGXS010000000
VERSION     DAZGXS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHKG5_000483"
     CDS             complement(142..837)
                     /gene="aqpZ"
                     /locus_tag="ACHKG5_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0866657.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHKG5_000484"
     CDS             1263..2921
                     /gene="ybjD"
                     /locus_tag="ACHKG5_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0866658.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHKG5_000485"
     CDS             complement(2918..3874)
                     /gene="ybjX"
                     /locus_tag="ACHKG5_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0866659.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4025..5140
                     /gene="macA"
                     /locus_tag="ACHKG5_000486"
     CDS             4025..5140
                     /gene="macA"
                     /locus_tag="ACHKG5_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0866660.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5137..7083
                     /gene="macB"
                     /locus_tag="ACHKG5_000487"
     CDS             5137..7083
                     /gene="macB"
                     /locus_tag="ACHKG5_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0866661.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHKG5_000488"
     CDS             complement(7156..7380)
                     /gene="cspD"
                     /locus_tag="ACHKG5_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0866662.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7530..7604
                     /gene="yljB"
                     /locus_tag="ACHKG5_000489"
     CDS             7530..7604
                     /gene="yljB"
                     /locus_tag="ACHKG5_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0866663.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7785..9023
                     /locus_tag="ACHKG5_000490"
     CDS             7785..9023
                     /locus_tag="ACHKG5_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0866664.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDKKIVVMTPRIYSQNS"
     gene            9434..9643
                     /locus_tag="ACHKG5_000491"
     CDS             9434..9643
                     /locus_tag="ACHKG5_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0866665.1"
                     /translation="MREINEDRVIREKECRELTGVCRTTRYEMEKQGRFPSRISLGGR
                     SVGWVKSEVMEWVKSRNRINSGKAA"
     gene            complement(9778..9957)
                     /locus_tag="ACHKG5_000492"
     CDS             complement(9778..9957)
                     /locus_tag="ACHKG5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0866666.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKEQE"
     gene            10148..10351
                     /locus_tag="ACHKG5_000493"
     CDS             10148..10351
                     /locus_tag="ACHKG5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0866667.1"
                     /translation="MLNHTTHPQGRDSHNLNKYIWRFLALSAGESQIIHVTAWTEREA
                     RSRCPSGCVAVFVARIRQGVCRA"
     gene            10344..10529
                     /locus_tag="ACHKG5_000494"
     CDS             10344..10529
                     /locus_tag="ACHKG5_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866668.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHTVTCIQDEE"
     gene            10533..10724
                     /locus_tag="ACHKG5_000495"
     CDS             10533..10724
                     /locus_tag="ACHKG5_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866669.1"
                     /translation="MQEFTLNEIAERAQQVEIICRLLEVYPDKLTDDDISALAGLLAK
                     LSGSVALWLIDERFKRYEQ"
     gene            10714..10956
                     /locus_tag="ACHKG5_000496"
     CDS             10714..10956
                     /locus_tag="ACHKG5_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866670.1"
                     /translation="MNNNKRVICELNELTQSAISYNNAVSEIINVWFESLTNCERDES
                     TTRLTFAISELLNISTSKIESISEKINGLSGHAIGE"
     gene            10962..11261
                     /locus_tag="ACHKG5_000497"
     CDS             10962..11261
                     /locus_tag="ACHKG5_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0866671.1"
                     /translation="MKLKYPGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIKKMLNVFRGKK"
     gene            11258..13012
                     /locus_tag="ACHKG5_000498"
     CDS             11258..13012
                     /locus_tag="ACHKG5_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFP0866672.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALQIVPDGKKRVRLYQAGELDLVETKKIGQKLAAAGVQDA
                     NFYPEGMHGQKVENWREYLARERQNLSEGLVVELPVKQKAQLSQMADSERAQLLSDRF
                     DGVCVHPESEIVHVWRGGLWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLDHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTTVIRAVVLATNNNPMIFTERAGGVSRRRVIFRFDNIVSEAEKDKDLPEKVAAE
                     IPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVAEFSKAMKPAAKVYGHEYITRRV
                     KGVTQTNAITTDDCDAFL"
     gene            13299..13550
                     /locus_tag="ACHKG5_000499"
     CDS             13299..13550
                     /locus_tag="ACHKG5_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866673.1"
                     /translation="MHTSGKLNNQKKPHNRAIDLTEHWLRVAIKIIDRHTREGYAKEN
                     PELISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            13681..13875
                     /locus_tag="ACHKG5_000500"
     CDS             13681..13875
                     /locus_tag="ACHKG5_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866674.1"
                     /translation="MEEIKQSATVMIDHEILNELKKIQFDVIRRMNEAGIYGRAATPT
                     IGHLARVLLRERLGIATGEA"
     gene            13879..14040
                     /locus_tag="ACHKG5_000501"
     CDS             13879..14040
                     /locus_tag="ACHKG5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFP0866675.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSNEVE
                     GLISLLGEQ"
     gene            14171..14659
                     /locus_tag="ACHKG5_000502"
     CDS             14171..14659
                     /locus_tag="ACHKG5_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0866676.1"
                     /translation="MALTTKKKRFADALSRGANPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            14671..14832
                     /locus_tag="ACHKG5_000503"
     CDS             14671..14832
                     /locus_tag="ACHKG5_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866677.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            14822..15745
                     /locus_tag="ACHKG5_000504"
     CDS             14822..15745
                     /locus_tag="ACHKG5_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0866678.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEMTKRNDPAVSKFLE
                     KSIVNRMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLHSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIEIFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            16724..17044
                     /gene="clpS"
                     /locus_tag="ACHKG5_000505"
     CDS             16724..17044
                     /gene="clpS"
                     /locus_tag="ACHKG5_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0866679.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            17075..19351
                     /gene="clpA"
                     /locus_tag="ACHKG5_000506"
     CDS             17075..19351
                     /gene="clpA"
                     /locus_tag="ACHKG5_000506"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0866680.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(19697..19784)
                     /locus_tag="ACHKG5_000507"
     tRNA            complement(19697..19784)
                     /locus_tag="ACHKG5_000507"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19748..19750),aa:Ser,seq:gga)
     gene            complement(20038..20256)
                     /gene="infA"
                     /locus_tag="ACHKG5_000508"
     CDS             complement(20038..20256)
                     /gene="infA"
                     /locus_tag="ACHKG5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0866681.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(20541..21245)
                     /gene="aat"
                     /locus_tag="ACHKG5_000509"
     CDS             complement(20541..21245)
                     /gene="aat"
                     /locus_tag="ACHKG5_000509"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0866682.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(21287..23008)
                     /gene="cydC"
                     /locus_tag="ACHKG5_000510"
     CDS             complement(21287..23008)
                     /gene="cydC"
                     /locus_tag="ACHKG5_000510"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0866683.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(23009..24775)
                     /gene="cydD"
                     /locus_tag="ACHKG5_000511"
     CDS             complement(23009..24775)
                     /gene="cydD"
                     /locus_tag="ACHKG5_000511"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0866684.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(24898..25863)
                     /gene="trxB"
                     /locus_tag="ACHKG5_000512"
     CDS             complement(24898..25863)
                     /gene="trxB"
                     /locus_tag="ACHKG5_000512"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0866685.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            26408..26902
                     /gene="lrp"
                     /locus_tag="ACHKG5_000513"
     CDS             26408..26902
                     /gene="lrp"
                     /locus_tag="ACHKG5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0866686.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            27037..31065
                     /gene="ftsK"
                     /locus_tag="ACHKG5_000514"
     CDS             27037..31065
                     /gene="ftsK"
                     /locus_tag="ACHKG5_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0866687.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            31220..31831
                     /gene="lolA"
                     /locus_tag="ACHKG5_000515"
     CDS             31220..31831
                     /gene="lolA"
                     /locus_tag="ACHKG5_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0866688.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            31842..33185
                     /gene="rarA"
                     /locus_tag="ACHKG5_000516"
                     /gene_synonym="mgsA"
     CDS             31842..33185
                     /gene="rarA"
                     /locus_tag="ACHKG5_000516"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0866689.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            33276..34568
                     /gene="serS"
                     /locus_tag="ACHKG5_000517"
     CDS             33276..34568
                     /gene="serS"
                     /locus_tag="ACHKG5_000517"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0866690.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            34807..37251
                     /gene="dmsA"
                     /locus_tag="ACHKG5_000518"
     CDS             34807..37251
                     /gene="dmsA"
                     /locus_tag="ACHKG5_000518"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0866691.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            37262..37879
                     /gene="dmsB"
                     /locus_tag="ACHKG5_000519"
     CDS             37262..37879
                     /gene="dmsB"
                     /locus_tag="ACHKG5_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0866692.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            37881..38744
                     /gene="dmsC"
                     /locus_tag="ACHKG5_000520"
     CDS             37881..38744
                     /gene="dmsC"
                     /locus_tag="ACHKG5_000520"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0866693.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(38780..39406)
                     /gene="ycaC"
                     /locus_tag="ACHKG5_000521"
     CDS             complement(38780..39406)
                     /gene="ycaC"
                     /locus_tag="ACHKG5_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0866694.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            39721..40869
                     /gene="ycaD"
                     /locus_tag="ACHKG5_000522"
     CDS             39721..40869
                     /gene="ycaD"
                     /locus_tag="ACHKG5_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0866695.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            41079..42509
                     /gene="ycaM"
                     /locus_tag="ACHKG5_000523"
     CDS             41079..42509
                     /gene="ycaM"
                     /locus_tag="ACHKG5_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0866696.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(42510..42620)
                     /locus_tag="ACHKG5_000524"
                     /pseudo
     CDS             complement(42510..42620)
                     /locus_tag="ACHKG5_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(42719..43459)
                     /gene="pflA"
                     /locus_tag="ACHKG5_000525"
     CDS             complement(42719..43459)
                     /gene="pflA"
                     /locus_tag="ACHKG5_000525"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0866697.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(43651..45933)
                     /gene="pflB"
                     /locus_tag="ACHKG5_000526"
     CDS             complement(43651..45933)
                     /gene="pflB"
                     /locus_tag="ACHKG5_000526"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0866698.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(45988..46845)
                     /gene="focA"
                     /locus_tag="ACHKG5_000527"
     CDS             complement(45988..46845)
                     /gene="focA"
                     /locus_tag="ACHKG5_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0866699.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(47251..49011)
                     /gene="ycaO"
                     /locus_tag="ACHKG5_000528"
     CDS             complement(47251..49011)
                     /gene="ycaO"
                     /locus_tag="ACHKG5_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0866700.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            49141..49833
                     /gene="ycaP"
                     /locus_tag="ACHKG5_000529"
     CDS             49141..49833
                     /gene="ycaP"
                     /locus_tag="ACHKG5_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0866701.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            50032..51120
                     /gene="serC"
                     /locus_tag="ACHKG5_000530"
     CDS             50032..51120
                     /gene="serC"
                     /locus_tag="ACHKG5_000530"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0866702.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            51191..52474
                     /gene="aroA"
                     /locus_tag="ACHKG5_000531"
     CDS             51191..52474
                     /gene="aroA"
                     /locus_tag="ACHKG5_000531"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0866703.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            52643..53407
                     /gene="ycaL"
                     /locus_tag="ACHKG5_000532"
     CDS             52643..53407
                     /gene="ycaL"
                     /locus_tag="ACHKG5_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0866704.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            53580..54263
                     /gene="cmk"
                     /locus_tag="ACHKG5_000533"
     CDS             53580..54263
                     /gene="cmk"
                     /locus_tag="ACHKG5_000533"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0866705.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            54374..56047
                     /gene="rpsA"
                     /locus_tag="ACHKG5_000534"
     CDS             54374..56047
                     /gene="rpsA"
                     /locus_tag="ACHKG5_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0866706.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            56207..56491
                     /gene="ihfB"
                     /locus_tag="ACHKG5_000535"
     CDS             56207..56491
                     /gene="ihfB"
                     /locus_tag="ACHKG5_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0866707.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            56698..58962
                     /gene="ycaI"
                     /locus_tag="ACHKG5_000536"
     CDS             56698..58962
                     /gene="ycaI"
                     /locus_tag="ACHKG5_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP0866708.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            58999..60747
                     /gene="msbA"
                     /locus_tag="ACHKG5_000537"
     CDS             58999..60747
                     /gene="msbA"
                     /locus_tag="ACHKG5_000537"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0866709.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            60744..61730
                     /gene="lpxK"
                     /locus_tag="ACHKG5_000538"
     CDS             60744..61730
                     /gene="lpxK"
                     /locus_tag="ACHKG5_000538"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0866710.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            61767..62999
                     /gene="ycaQ"
                     /locus_tag="ACHKG5_000539"
     CDS             61767..62999
                     /gene="ycaQ"
                     /locus_tag="ACHKG5_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0866711.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            63051..63233
                     /gene="ycaR"
                     /locus_tag="ACHKG5_000540"
     CDS             63051..63233
                     /gene="ycaR"
                     /locus_tag="ACHKG5_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0866712.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            63230..63976
                     /gene="kdsB"
                     /locus_tag="ACHKG5_000541"
     CDS             63230..63976
                     /gene="kdsB"
                     /locus_tag="ACHKG5_000541"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0866713.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            64130..65023
                     /gene="ycbJ"
                     /locus_tag="ACHKG5_000542"
     CDS             64130..65023
                     /gene="ycbJ"
                     /locus_tag="ACHKG5_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0866714.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(65000..65779)
                     /gene="elyC"
                     /locus_tag="ACHKG5_000543"
     CDS             complement(65000..65779)
                     /gene="elyC"
                     /locus_tag="ACHKG5_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0866715.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            65915..66700
                     /gene="cmoM"
                     /locus_tag="ACHKG5_000544"
     CDS             65915..66700
                     /gene="cmoM"
                     /locus_tag="ACHKG5_000544"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0866716.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            66697..68019
                     /gene="mukF"
                     /locus_tag="ACHKG5_000545"
     CDS             66697..68019
                     /gene="mukF"
                     /locus_tag="ACHKG5_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0866717.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            68000..68704
                     /gene="mukE"
                     /locus_tag="ACHKG5_000546"
     CDS             68000..68704
                     /gene="mukE"
                     /locus_tag="ACHKG5_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0866718.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            68704..73164
                     /gene="mukB"
                     /locus_tag="ACHKG5_000547"
     CDS             68704..73164
                     /gene="mukB"
                     /locus_tag="ACHKG5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0866719.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            73425..75272
                     /gene="ldtD"
                     /locus_tag="ACHKG5_000548"
     CDS             73425..75272
                     /gene="ldtD"
                     /locus_tag="ACHKG5_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0866720.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            75453..76001
                     /gene="mepK"
                     /locus_tag="ACHKG5_000549"
     CDS             75453..76001
                     /gene="mepK"
                     /locus_tag="ACHKG5_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0866721.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            76028..76675
                     /gene="gloC"
                     /locus_tag="ACHKG5_000550"
     CDS             76028..76675
                     /gene="gloC"
                     /locus_tag="ACHKG5_000550"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0866722.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(76726..77916)
                     /gene="aspC"
                     /locus_tag="ACHKG5_000551"
     CDS             complement(76726..77916)
                     /gene="aspC"
                     /locus_tag="ACHKG5_000551"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0866723.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(78101..79189)
                     /gene="ompF"
                     /locus_tag="ACHKG5_000552"
     CDS             complement(78101..79189)
                     /gene="ompF"
                     /locus_tag="ACHKG5_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0866724.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT        19991 a        19145 c        20982 g        19218 t
ORIGIN      
        1 gtaagacgcg ccctcagccc acatccatgt gggctgcccc ggccttcggg gaacgcttca
       61 gcgattttga cgccaccaat acccgtgcgg tttgtaatgt cgaagataaa acaaccaggg
      121 cagataaaat gccaatgcta attaattacg cttttccagc agagtccggt aaatcagacc
      181 accgataatg ccaccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca
      241 gccgccctgg aagatggcaa ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac
      301 cggaatactg attaagtgaa tcagggttaa ggccagacca atagcgatcg gcgcaaaacc
      361 tgccggagcg aatttgtcgg ttgcaccgtg gatcactaac aggaagcccg cactcaatac
      421 cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc
      481 gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata
      541 cagcagcgct gccgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc
      601 ttttgccgga aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc
      661 accagaaata tgaccaacag caaaggccat cgtcagaacg gtcagaccga acgccaacgc
      721 cacgccggca aaaccaatgc ctaattccgg gaagcctgcg gccagtacag cactaccaca
      781 gccaccaaaa acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc
      841 cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa
      901 aagtgacgac ggaaataatg cgcggctatt ttaaaaacga aggcgagtca ttcaccagat
      961 aaataaatcc agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc
     1021 tgattgacag tgtagtgcaa gcaaaagatt taatccttta ggcgtaataa aaaataattt
     1081 atcatgctaa ttatttgatt ttgttgtttt tgcagactta tcagcaagag ggagtataac
     1141 gcgattattc gctcattttt cagacatttg ccatgcttaa atgtgatgtc atcacgtatt
     1201 agcaaggcct ttcccgttat actgccagcg taaaggataa gtcacatatt tctggagggg
     1261 atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga
     1321 tgctggaaca aaacaacgtc ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg
     1381 acgccttaac cctgctgcta tcgccagaat cagatctcta ccattttgag cgcgacgatt
     1441 tctggttccc gccgggagat atcaacgggc gagaacatca tctgcatatt attttgacct
     1501 tccgcgaatc gctgccaggt cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct
     1561 ggacgccatg caccgatggc tatcaccgta ttttttatcg tctggaaggg gagagtgcgg
     1621 aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa agacggacat ccgattgatg
     1681 ttgaggatat taacgatcag gcacaccatc tggtgcgttt aatgccggtg ctgcgcttgc
     1741 gtgatgcccg ttttatgcgc cgtattcgta acggcacggt gccaaatgtc cccaatgtgg
     1801 aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc
     1861 tctctgatgg acagattcgt cagggacttt ccgcaatggt gcagttgctt gagcattatt
     1921 tctctgagca gggggctgga caggcgcgat atcgtttaat gcggcggcgg gccagcaatg
     1981 agcaacgaag ctggcgctat ctggacatca tcaaccggat gattgaccga cctggagggc
     2041 gctcgtatcg ggttattttg ctcggcctgt ttgctacttt attgcaggca aaaggcacat
     2101 tgcgactgga taaagacgcc cgtccattgt tgctgatcga agatccagaa acgcgtttac
     2161 accccattat gctttcagtt gcctggcatc tgttgaatct tttgccttta caacgtattg
     2221 ctaccaccaa ctctggcgag ctgctttcgt taactccagt ggaacatgtt tgccgcctgg
     2281 tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc aagtggcttg agcaccgaag
     2341 acagccgccg catctctttt cacattcgtt ttaatcgtcc gtcatcgctg tttgctcgct
     2401 gctggttgct ggtggaaggg gaaacggaaa cctgggttat caatgaactg gcgcgtcagt
     2461 gcggacatca ttttgatgcc gaagggatca aggtcattga gtttgcccag tccgggctaa
     2521 agccactggt taaatttgct cgccgaatgg ggattgaatg gcatgtactg gtcgatggcg
     2581 atgaagcagg gaagaaatat gccgctacgg tacgcagcct gttgaataat gatcgggaag
     2641 ccgaacgaga acatttaacg gcgttaccgg cgctggatat ggaacatttt atgtatcgcc
     2701 agggattttc cgatgtgttc caccgcgtgg cgcaaatccc ggaaaatgtc ccgatgaatc
     2761 tgcgcaaaat tatctcgaaa gcgatccatc gctcttccaa acccgatctt gccattgaag
     2821 tggcaatgga ggccggacgt cgtggtgtgg actccgtacc gacgctgctg aaaaaaatgt
     2881 tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca
     2941 tttgtggctg aatagcgtcg agcatctcat agcgccgacg gtattcagcc cgttttttac
     3001 tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg aaggcggtaa tggcgttcag
     3061 catcacatac gccgccaacc gattcccaga aagcgttgta atcggcatgg atcttgcctt
     3121 ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc attgctgacg gcaataatct
     3181 gctctacctg caaacgttgg gcaaacagac aggccgcttc catcacgagg cgtttgggaa
     3241 atagcccgtg gcaggctttc gtcgcattct ggatttcctg atgtggaatt tcccattttg
     3301 cgccttgcag tccaccaata aacatcgttc ttttcccctg atattcacac agggtaaacg
     3361 tgatttctgc cagaggaata ccttcgctgt tgcggaacag gattgtgctg tcaccttctt
     3421 tatccattga gatcatcatg gtcagctcaa gcgtgaactg ctcgccgttt ttgccttcca
     3481 gcttcgccag ttgcagcccg ggggtattca aatataagct gaattcttcc gccgacatac
     3541 atccacggag taacgcataa tggtaacgta acgcctccag caattgctta cggctaagat
     3601 tcgccgcaag gtaagggcga tgcagacgca caggcagtcg cggctggcgc gttaacaata
     3661 cattgagatt aggccagtgg gaaagttcgt tcatccactc aacgcttaaa cgcggcataa
     3721 tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg atgccagaat ttacccggcc
     3781 gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct gctgagagat tcagatgacg
     3841 taaaggtccg ttcggttagc tgcgacatat tcatgaaatc aatggttgta catgacgtcg
     3901 atttcaccat tgcgtatctt aaccaaacat caatagtgtg attactaacg taaattttag
     3961 ggttttgttg atatttcgtt gaagttaatg acccggattg gcatatggag tattcagaat
     4021 atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat
     4081 cgccggactg attacgttat ggagaattct taacgcaccc gtgccgactt atcagacact
     4141 gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg accggaaagc tggacgcgct
     4201 gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg aaaacgctgt cggtggcgat
     4261 tggcgataaa gtaaaaaaag accagctttt aggggttatt gatcctgaac aggctgaaaa
     4321 ccagatcaag gaggtcgaag caacgctgat ggagctacgc gcgcagcggc agcaggcgga
     4381 agcggagctg aaactggcgc gggtgacgta ttcccgtcag caacgtctgg cacaaacgca
     4441 ggcggtttca ctgcaggatc tcgacaccgc cgcgacggag atggctgtga aacaggcgca
     4501 aattggcacc attgacgcgc aaatcaagcg caatcaggct tctctcgata cggctaaaac
     4561 caatctcgat tacactcgca tcgttgcccc gatggccggg gaagtcacgc aaatcaccac
     4621 tctgcaaggc cagacggtga ttgccgcaca acaagcaccg aacattctga cgctggcaga
     4681 tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg gatgtaatcc acctgaagcc
     4741 ggggcaaaaa gcctggttta cggtacttgg cgatccactg acgcgctacg aggggcaaat
     4801 caaggatgta ctaccgacgc cggaaaaggt taacgacgct attttctatt acgcccgttt
     4861 tgaagtcccc aaccccaatg gtttgctgcg gctggatatg actgcgcaag tgcatattca
     4921 gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg gcgttaggcg atccggttgg
     4981 cgataatcgt tataaagtca aattgttgcg taatggtgaa acacgcgagc gtgaagtgac
     5041 gattggcgca cgtaacgata ccgatgttga gattgtcaaa gggctggaag cgggcgatga
     5101 agtggtgatt ggtgaggcca aaccaggagc tgcacaatga cgcctttgct cgaattaaag
     5161 gatattcgtc gcagctatcc tgccggtgat gagcaggttg aggtgctgaa gggcatcacc
     5221 ctcgatattt atgccggtga gatggtagcg attgttggcg cttcgggttc cggtaaatcg
     5281 accctgatga atattctcgg ctgtctggat aaggccacca gcggcaccta tcgcgtcgcc
     5341 ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc aactgcgccg cgagcatttc
     5401 ggctttattt tccagcgtta ccatttgctt tcgcatttaa ccgccgagca gaacgttgaa
     5461 gtaccagccg tctatgctgg tcttgagcgg aaacagcggc tgcttcgcgc ccaggagttg
     5521 ctgcaacgac tggggctgga agaccgtaca gagtattatc cggcacagct ttcgggtggt
     5581 cagcaacagc gcgttagcat cgcgcgggca ttgatgaacg gcggtcaggt aattcttgcc
     5641 gatgaaccga ccggcgcact ggacagccat tcaggcgaag aggtgatggc gatcctgcat
     5701 cagctgcgcg atcgcgggca tacggtgatt atcgtcactc acgatccgca ggtcgctgct
     5761 caggccgagc gggtgatcga aattcgcgac ggcgaaattg tgcgcaatcc tcccgccatt
     5821 gaaaaagtga atgttgctgg cgggacggaa cctgttgtca acacggcgtc tggctggcgg
     5881 cagtttgtca gcggttttaa tgaggcgctg acgatggcat ggcgggcgct ggcagcgaat
     5941 aaaatgcgta ctttactgac catgctgggg attataatcg gtattgcgtc ggtggtttcc
     6001 attgtcgtgg tgggcgacgc cgccaaacaa atggtgctgg cggatattcg ttctattggt
     6061 acgaatacta ttgatgtcta tcccgggaaa gattttggcg atgacgatcc gcaatatcaa
     6121 caggcgctga agtacgatga cttaatcgcc atccaaaaac aaccgtgggt cgcttcagcc
     6181 acacctgccg tctcgcaaaa cctgcgcctg cgttataaca atgttgatgt tgctgccagt
     6241 gccaatggcg tgagcggcga ttattttaat gtctatggca tgaccttcag tgaaggaaac
     6301 acctttaacc aggaacagct gaacggtcgt gcgcaggtcg tggttctcga cagtaatact
     6361 cgccgccagc ttttccccca taaagcagat gtggttggcg aggtgattct ggtcggcaat
     6421 atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa
     6481 gtgctgcgtg tctggctacc ttacagcacg atgtccgggc gagttatggg ccagtcgtgg
     6541 ctgaactcca ttactgtcag ggtgaaagaa ggatttgaca gcgccgaggc ggaacagcaa
     6601 ctcacgcgtt tactttcact gcgccacggt aagaaggatt tctttacctg gaacatggac
     6661 ggcgtcttga aaaccgttga aaagaccaca cgtactttac aactgtttat gacgctggtg
     6721 gcggtgattt cgctggtagt gggcggtatt ggtgtaatga atattatgct ggtgtcagtg
     6781 accgagcgga cgcgggaaat tggcattcgc atggctgtag gtgcgcgagc aagcgatgtt
     6841 ttgcaacagt tcctgatcga agccgtactg gtttgcctgg tcggtggcgc gttgggaata
     6901 acactgtcac tgttaattgc tttcaccttg cagcttttct tacccggctg ggagattggt
     6961 ttttcaccgt tggcactgct gctggcgttt ctctgctcga cggtcaccgg gattttattt
     7021 ggctggttac ccgcacgaaa tgcggcacga ctggatccag tagatgctct ggcacgagag
     7081 taatttttga gataaaaatg ccagccgatc gggctggcat tttgccttta ggatgtacac
     7141 aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa taacactggc
     7201 gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag cttttagcgt
     7261 tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc cgccgccttc
     7321 agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac ccttttccat
     7381 gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg gtcagagagg
     7441 gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg gcgagccgtc
     7501 aagcatttga caggggacag ggggcaggta tgaatcaaaa atttgaagca gttaacgcta
     7561 ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta tttatcagtt
     7621 agtgttcgtg ggtgttcgtc atggtgtggg catctaattt atttctatga ttataaagga
     7681 taatttcgtt tttcttgttc gcccttgttc gtcacagttc gcccttgtac gatgcacggt
     7741 gtagtcaatg gtgtagttaa ttttgatgac cggaaacacg aaacatgagc agggcaattc
     7801 atagacttag cgacactctt ttacgaaaat taagcggatc accaaccaca aaaagcacct
     7861 tttttaatga tggtggcaac cttagcgtaa ggcattcaac caatggcctg ttaacctggt
     7921 atttcactta caggtccgga actggtaggc agatatcacc tgaacgtctg aggctgggaa
     7981 attatccaga tttgagcctg aaagcagcca gggaaaaagc gtcgcaatgt cgcgcatggc
     8041 tggctgaggg gaaaaatccg cgctatgaac ttaatcatgc tgtacaggac gcgttagccc
     8101 ccgtgacggt taaggaagcg ctcacttact ggcttgaatc gtacgcaaag gaaaagcgca
     8161 ctgactacga atcactgaaa agccggatca ataaacacat aattagccag attggagcta
     8221 tgccactgga aaaatgcgag ctacgccact ggctggcctg ttttgaccag cttgctaagc
     8281 gtaatccggt atcagccgga tttttgctac aggtatgcaa acaggcgctt aagtactgcc
     8341 gaaaacgacg atacgcaatc agcaacgttc ttgatgatat ggttgtcggt gatgttggga
     8401 aaaaagcaga aataagcgag cgcgtactaa caaacaagga gcttggggaa ttactacgcg
     8461 cccttgatga aaaaatttac ccaccgtact acagcgccct gattcgcctc ctgattgtct
     8521 tcggatgtcg taccactgag ctaagacgct ctgaggtcca ggaatgggat tttaaagaaa
     8581 tgctctggac cgtgccgaaa gaacacagca aaacgaaggt agcaatattc aggccaatac
     8641 cggaagcgat cttgccgttt gtcatgaagc tggtggagca gaacaggcac acgggcttat
     8701 tgctcggaga actgaaaggg caatcatccg tatcagagta cggaagaacg gcacacagac
     8761 gcattaatca agccccctgg acgttgcacg acatccggca cacgtttaca acgatgctga
     8821 acgatttagg cgtggaaccg catgttgtgg agcagcttac cgcgcaccag ttgccaggaa
     8881 tgcaacgagt ctataatcac tcccgttatc ttgatgcaaa acgtgacgct cttaatctat
     8941 gggttgagcg tctcgagctt ctccagaaca atgataaaaa aatcgttgtt atgaccccgc
     9001 gaatttactc ccaaaattct tgacaaatta tggctgtttt tcttgcgaaa aatggccctc
     9061 taaaataaat cgtcgaagtt cgaccaaagt tcgtcgtttt tttatttgtc aatcatcgaa
     9121 aaaattgaaa ttcactctcc tgaatcctta ttctctgttt cttgattgtt ccaacacctg
     9181 atggtttatc tatttgtttt taatgagata atttaagtgt aataccaaat gacgacattt
     9241 ttttgttgcg tcgcttaaaa actcgtgaca cgattttatg aacagcagca aactctagcg
     9301 aactttgacg aacataaagt tcagaaaggt tacgtattca atttaagagg taagaaaagc
     9361 taagaagtaa tatctttccg tatatccaca gcgaaacaaa aatttctata cgtaatttta
     9421 tgtaggactt tttatgcgcg aaataaatga agatcgtgta atccgcgaaa aagaatgcag
     9481 agagctaaca ggcgtttgcc gcacaactcg ttacgagatg gaaaagcagg gccgcttccc
     9541 ctcacgtatc agtctcggcg gtcgttctgt tggctgggtt aagtctgagg ttatggaatg
     9601 ggttaaaagc agaaatcgta ttaattcagg taaggcagcg tagggggaac atatgacaca
     9661 taaaacaaag gcgacctgtg gcggtcgcca atgggaaaac actaaacata agccagaaca
     9721 tcatagcgat ttgctgtctg gtgggcaatg tgctcagtgt gctggcttgc tggtgggcta
     9781 ttcctgctct ttggccttgc ggcgctggcg gcgtttgatt tcgcctttaa cggcagtaac
     9841 tataaattgc gctttgcttt caccttcatc taaatttttt tctaagtctg caacaacatc
     9901 atgcgggaat ctggcattta actgttgcga cttattattt gttgaacctg ttgccattac
     9961 tgaatcctct ctaagttggt gcgattcagt atacacaaaa aaaattcaga gaaaaggctt
    10021 gaagtgcgat tcacttgctg ataggttaaa aacagatggt gcgatgcacc aaataacaac
    10081 gccccgcagt ggtggcacac atgcagggcg tctaacaacc aacgatagca acagtatcga
    10141 ggtagctatg ttaaatcata ccacacaccc gcaagggcgg gactcgcaca acctgaataa
    10201 atacatctgg cgttttctgg ccctgagtgc cggagaatct caaatcatcc acgtaaccgc
    10261 ctggacggaa cgcgaagcgc gtagccgttg cccgtcaggt tgtgttgctg tatttgtcgc
    10321 ccgtattcgt cagggggtgt gccgtgctta aaaccttccg cgttttttcc cgtgctgtta
    10381 acaagcgcgg ctataccgtt ggcatcacgc agaacgtgaa ggccgaaaac gtccaggctg
    10441 caatcagcac agtaattaac aaatcagcta aagccggttt atccggcgta attatccaca
    10501 cagtgacatg tattcaggat gaggagtaat taatgcagga gtttacatta aacgaaattg
    10561 cggaaagagc gcagcaggtt gaaatcattt gccgccttct tgaggtatac cctgacaaac
    10621 ttacggatga cgatatatcc gcattagctg gccttctggc aaaactttct ggaagtgttg
    10681 cgctgtggtt aattgatgag agatttaaac gctatgaaca ataacaaacg cgtaatttgc
    10741 gaattgaatg aactcacaca aagcgcaata tcttacaaca atgccgtaag tgagattatt
    10801 aacgtctggt ttgaatcgct gacaaattgc gagcgtgacg agtcaacgac aagattaacc
    10861 tttgccattt ctgaattact gaatatttcc accagcaaaa tagagagcat atcagagaag
    10921 attaacggct tatcaggtca cgctatcgga gaataaaaat aatgaaactt aaatatcctg
    10981 gcttaactgc cagcggcaaa actcgcacta aattcatgtg cggggatatt taccgcgata
    11041 agtacggcgg cacggtaatg attaagggcg tggaggaacg gcgcgtaact taccgccgtg
    11101 aaggctacga atatgattgc gttatgcctg tttatcagtt cgatcgtgat ttttctctgg
    11161 tgcaggctgt accgcgtaac gtgcccacca gcagggagaa agcacgcgcc aatattcagg
    11221 aaataaaaaa gatgcttaac gtattcaggg gtaagaaatg aaactggcac cgaacgtaaa
    11281 aaaacagcca cgcggaataa aacacaaaga cacagaggtg attattttcg cgggtagtga
    11341 tgcgtgggca catgcaaaac agtggcagga gcaggacggc cccgcatccg gcgataatgt
    11401 gcctcctgtg tggcttgggc caaatcagct tgccgaactt gatgcactgc aaattgttcc
    11461 tgatggaaaa aaacgcgtaa ggctgtacca ggcaggagaa ctggacttgg tggagaccaa
    11521 aaagattggt cagaagctgg cggcggcagg tgtacaggat gcaaattttt accccgaagg
    11581 aatgcacggt cagaaggtgg agaactggcg cgaatatctt gcccgtgagc gccagaatct
    11641 ttctgaaggt ctggtcgttg agcttccggt aaagcaaaag gcgcaacttt cgcagatggc
    11701 ggacagtgaa cgcgctcagc tgctttccga tcgctttgat ggcgtttgcg tacatcctga
    11761 aagtgaaatc gttcacgtat ggcgcggcgg gttatggtgt ccggtcagca caatggaact
    11821 tagccgcgaa atggtggcga tctattcaga gcacagggcc actttcagca aacgcgtgat
    11881 caataacgcc gttgaagcgt taaaagttat tgccgaacca atgggggagc cgtccggcga
    11941 tttgctgccg ttcgccaatg gtgcgcttga cctgaaaacg ggggaatttt ccccgcacac
    12001 gccggagaac tggatcacca cgcacaacgg cattgagtac acgccaccag cacctgggga
    12061 gaacattcgc gacaacgcgc caaactttca caaatggctt gatcacgcag ccggaaaaga
    12121 cccgcgcaag atgatgcgta tatgtgccgc gctgtacatg attatggcga accggtacga
    12181 ctggcagatg tttattgagg ccaccggaga cggcggtagc ggtaaaagta cattcacgca
    12241 catagccagc cttctggcag ggaaacagaa cacggtaagc gctgaaatga catcgcttga
    12301 tgatgctggt ggacgtgcgc aggttgtcgg gagtcgtctt atcgtcctgg ctgaccaacc
    12361 gaaatataca ggcgaaggaa cgggcatcaa gaaaatcacg ggcggcgacc ccgtcgaaat
    12421 taacccgaaa tatgagaagc gattcacgac ggtaataagg gcggtggtac tggcgaccaa
    12481 caacaacccg atgatattca ccgagcgggc cggaggtgtg tcacgccgtc gggtgatttt
    12541 tcgctttgac aatatcgtta gcgaggcaga aaaggacaag gatttgccgg agaaggtcgc
    12601 ggctgaaatc cctgtgatta tccgccgatt actggcgaac tttgccgacc ctgaaaaggc
    12661 acgggcttta ctcctggaac agcgcgacgg tgatgaagca ctggcaataa agcaacagac
    12721 ggatccggtt attgagtttt gccagttcct gaattttctg gaggaagcgc gcggcctgat
    12781 gatgggtggc ggtggcgatt cagtgaagta cacgaccagg aacagccttt accgcgtcta
    12841 tctggcgttt atggcatacg caggaagaag caagccgcta aacgtggctg agttcagcaa
    12901 ggccatgaag ccagcggcaa aagtttacgg acatgaatat attacgcgga gagttaaggg
    12961 agtaacgcag accaacgcaa ttacaactga tgattgcgac gcgtttttat aattttagac
    13021 aaaagccctc tacctcttct acctaaagaa aataaatata aattattcaa tatgataagt
    13081 gggtagaggg gcaggtagaa ggcaaataaa agccctctac accctccacc atgattaagg
    13141 tcgtttgtga ggagcgggta gaggatgggt agagggccac gaaagccctc tacccatctg
    13201 aaagccgcgc cattactgac ctgaaagatg attgggtaga gaggtagagg aggtgcacca
    13261 caacctaaaa ctttttaaac acgagggtaa aaataaaaat gcacacatca ggaaaattga
    13321 acaatcagaa gaaaccacat aaccgcgcca ttgaccttac agagcactgg ctgagggtgg
    13381 cgataaaaat catcgaccgc cacacacggg aaggatacgc gaaggagaat cccgaactga
    13441 taagcgcatt catgaccaca gcggcggcaa actttgccac gctgacagaa cgggagattg
    13501 ccgaagcgga acaggtaaca accatcaacg ttaaaagcgc tggagaataa ccgaagtagt
    13561 gaacacatag ccggagcaat ccggcttttt ttcgatctct catatcttaa aaaatggttg
    13621 attgattata atctatcgaa aatactgatt attagatgat aagcatcagg aggaaacaaa
    13681 gtggaagaga taaagcaaag cgctaccgtg atgattgatc acgagatcct aaacgagctt
    13741 aaaaaaattc aattcgatgt tattcgccgg atgaatgaag ctggcatcta cggcagagcc
    13801 gccacgccaa caataggtca tcttgcacgt gtgctgttac gcgaaagact gggcattgca
    13861 acaggtgagg cataacaaat ggctatcaca gaacgcgagg caagcatcat taaggccatt
    13921 ggcgaggaaa caagagacac catagcgcca ttgatggaaa agatcgcaga gcttgagaca
    13981 cgaataaaca agctgagtaa tgaagttgaa ggcctgatct cgttactcgg tgagcaatga
    14041 catggggggg ggagtgttcc ggccggaaag tcgatcaccc tggacaccgc cccccgtctc
    14101 attcgtagaa aattttgctt ttcaaaaaag ttacacgaaa agttacaccc cctcaaaacg
    14161 aggtttttgc atggcattaa cgaccaaaaa aaagcggttt gctgatgcgt tatcaagggg
    14221 cgcaaatccc acggaagccg ctatacatgc gggatacagc gaaaagaccg caagggttaa
    14281 aggctggcag ctatccaacg atcctgaggt gaaaaagtat cttaaggctg taacttctga
    14341 tgtaactttt caggttacaa aaccgaaaaa agttacaaag aaagttaccg aaaaagttac
    14401 accggaacgg gcatcaatta acaccattga gatgatggat aacggcctac ccgaccccat
    14461 caaagcaatg ggcaggatac tgattgacaa catggacaca gatccaaaac tggctttaga
    14521 tgccgcgttt aagctggccc agttcaccca ccgcaagatc ggcagcattg ggaaaaaaga
    14581 ggttaaaaaa ctcaatgccg ctagtgtcta ctcaaatcgg ttccccatcc ctaagccacc
    14641 aacagactta aaacactgag gtagcgaact atgagcgaat acatcacaga ccctattgat
    14701 gtactcacgg aaatcatgaa cgacaagcag cagtcgaccg aaatacggtt acaggccgcc
    14761 gctgttctga tgccatattt tcacgaaaca ctaaacccgt gcttccagga ggaggaagaa
    14821 gatgatctgt aaaaacatga aaggcgtgtc agcagttcgc gccgccagcg tgctggcatc
    14881 gtgtaaattt agccatgctg acgctataga gatgacgaaa agaaatgatc cggcagtatc
    14941 gaaatttctt gaaaaatcca tcgtcaacag aatgactacg gataacatgc cgtcgctggt
    15001 ggcagagccg atagtaagag agcttggcga gtatattttt tcgcagtctg taccaggcaa
    15061 gctaatcagc agcgcgctta atcttccgtt tggcgcaaaa ttgcattcaa tatcaggcaa
    15121 gggtagtaaa tggcttaaag aagccgaaga aatccctgtc aaagagggag aagtaaacga
    15181 aagtgcgcta aaattgtgca aacttggcgc tattgtgatg ctgaataacg agctaatcaa
    15241 atttagcacc cctggtacaa attcagcgat cagagacttg atcacatcgg aatcggtgaa
    15301 agaaatagat aaaaaatttc tttccagtga tcaggaggtg gctggcgtat cccctgaggg
    15361 cgtgcttaat ggtgctgatg aggccgaaaa cttcatagag attttcaaaa agcacatagc
    15421 gaacggtaac acgctttcca cgtcgtcgct aatattgccc gtagaaaacg ccttacagct
    15481 tactgatgct gagttctggc agatagagct tttgagaata ccggtaattg tgagcgaaca
    15541 ggcggatcgc atgatgctga tagacgctca aaagctgatt attaacgttg aagccacggt
    15601 tataacccca actgatgagg cggtaataaa aaccgaatca ggggcggtta gccttttcca
    15661 gaatgacgct acagcgttca gggcaatcac ctattgcgga tggcaaaaac tcgataaggc
    15721 cgtcaccgtt ctggcgaaaa gctgattttt ccagcatcta tcccgatatg gggatcacca
    15781 tatcggggaa gcacctgaat catcattacc gtgatcaagg gtatgtaaag cccaccagcc
    15841 tgattaacgt ttaaccggaa aaaaagccag gtatccaatc tcgatatggg gatcaccata
    15901 tcgggatacg gcggtaactg gttgatattt tcgaaattcg ccagcggcga acatcgggga
    15961 aaccagccta acccgttgat tcttcccaat ttccacagac tgcggaaatt ggcgcggtaa
    16021 cttactgaga ttaaagcaaa gcgcaaaatt gcgttggctg gtgggcctga tgccgataac
    16081 tggttggaat tgttccatag tgcaaaattg cactatggcc taacccattg attatttcga
    16141 aaacccccat tgggggatgt tgggaaagta agactaaccc attgattctt ccacaatcct
    16201 caatttgagg agatcggaaa catcaccctt caaattctga atggtcataa tgaccacgca
    16261 gaccatcaag gcaaccacaa cgaacatcgt tacggttgga gcttttaccc cactggggaa
    16321 aaggtacgca cctgaatcat cattaccgtg atcaagggta tgtaaaggcc accagcctga
    16381 ttaacattta accggaaaaa aagccaggta ggtggggata cccatatcgg ggaacatcca
    16441 ccagcttcat aacggatggc cggaaaatga taacggttaa agcgtgttga tctttgttcg
    16501 tggttgttcg atattgttcg tgaaacggtg tagttaatgg tgtagttaat ttcgctgttt
    16561 ttgacggagt tgttatttgt aacgtagtga aaaataaaga ctaatcaatg aaatgttata
    16621 aatgatagtt atctatcatg tggagtagat tggtcaggca aataagctct tgtcagcggc
    16681 agggcgttct gccgataacc gtaaccgaag atgataactg acaatgggta aaacgaacga
    16741 ctggctggac tttgatcaac tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc
    16801 tatgtataaa gtgatattag tcaatgatga ttacactccg atggagtttg ttattgacgt
    16861 gttacaaaaa ttcttttctt atgatgtaga acgtgcaacg caattgatgc tcgctgttca
    16921 ctaccagggg aaggccattt gcggagtctt taccgccgag gttgcagaaa ccaaagtggc
    16981 gatggtgaac aagtacgcga gggagaatga gcatccattg ctgtgtacgc tagaaaaagc
    17041 ctgaatgcag gcataaaaat tgggggaggt gcctatgctc aatcaagaac tggaactcag
    17101 tttaaatatg gctttcgcca gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca
    17161 cttgttactg gcgctgctca gtaacccatc tgcccgggag gcgctggaag cgtgttctgt
    17221 ggatttggtt gcgctccgtc aggaactgga agcctttatt gaacaaacca cacccgttct
    17281 gcctgccagt gaagaggagc gcgacacaca gccgacgctg agttttcagc gtgtactgca
    17341 acgtgcggtc ttccatgtcc agtcctccgg tcgcaatgag gttaccggtg caaacgttct
    17401 ggtcgctatc tttagcgaac aggagtcgca ggcggcatat ctgttgcgta aacatgaagt
    17461 cagccgtctc gatgtggtga actttatctc tcatggcacg cgtaaagacg agccgacaca
    17521 gtcttctgat cctggcagcc agccaaacag cgaagaacaa gctggtgggg aggaacgtat
    17581 ggagaatttc acgacgaacc tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat
    17641 tggtcgtgag aaggagctgg agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa
    17701 cccgctgcta gtgggggaat ctggtgtcgg taaaaccgcg attgcagaag gtcttgcctg
    17761 gcgaattgtt cagggcgatg tgccggaagt gatggctgac tgtacgattt actctctcga
    17821 tatcggttct ctgttagcgg gcacaaaata tcgcggcgac tttgaaaaac gttttaaagc
    17881 gttgctcaag cagctggagc aggacactaa cagcatcctg tttattgatg agatccacac
    17941 cattatcggt gcgggtgcag cgtctggtgg tcaggttgat gcggctaacc taatcaaacc
    18001 gttgctctcc agcggtaaaa ttcgcgtaat tggttcgaca acctatcagg agttcagcaa
    18061 cattttcgag aaagaccgtg ctctggcgcg tcgcttccag aaaattgata ttactgaacc
    18121 gtcgatcgaa gaaactgttc aaatcatcaa tggcctgaaa ccgaagtatg aagcacacca
    18181 cgacgtgcgt tataccgcaa aagcggtgcg tgcggcggta gagctggcgg tgaaatacat
    18241 taacgatcgt catctgccgg ataaagccat tgatgttatc gacgaagcgg gcgctcgcgc
    18301 acgtctgatg ccggtaagca aacgcaagaa aaccgttaat gtggcggata ttgagtccgt
    18361 ggtggcccgt attgcacgca ttccagagaa gagtgtttct cagagtgacc gcgataccct
    18421 gaaaaacctc ggcgatcgcc tgaaaatgct ggtcttcggt caggataaag ccattgaggc
    18481 gctgactgaa gccattaaga tggcgcgtgc aggtttaggt cacgaacata aaccggtcgg
    18541 ttcgttcctg tttgccggcc ctaccggggt cgggaaaaca gaggtgacgg tacagctttc
    18601 gaaagctttg ggcattgagc ttctgcgctt tgatatgtcc gagtatatgg aacgccatac
    18661 cgtcagccgt cttattggtg cgcctccggg atacgttggt tttgatcagg gcggtttgct
    18721 gactgatgcg gtcatcaagc atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc
    18781 gcacccggac gtgttcaata ttctgttgca ggtgatggac aacggtacgc tgaccgataa
    18841 caacggacgc aaagcggact tccgtaacgt ggtactggtg atgaccacta acgccggggt
    18901 acgggaaact gagcgcaaat ccattggtct tatccaccag gataacagca ccgatgcgat
    18961 ggaagagatc aagaagatct ttacaccgga attccgtaac cgtctcgaca acattatctg
    19021 gttcgatcat ctgtcaactg acgtgatcca tcaggtggtg gataaattca tcgtcgagtt
    19081 gcaggttcag ctggatcaga aaggtgtttc tctggaagtg agccaggaag cgcgtaactg
    19141 gctggccgag aaaggttacg accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca
    19201 ggacaacctg aaaaaaccgc tcgccaacga actgctgttt ggttcgctgg tggacggcgg
    19261 tcaggtcacc gtcgcgctgg ataaagagaa aaatgagctg acttacggat tccagagtgc
    19321 acaaaagcac aaggcggaag cagcgcatta atctgattgt caggtaggtt ggtgaagtcc
    19381 gtaatctcga aagaggttac ggacttttta tttatggggt ggaacggtga aaaccctatt
    19441 tttggaggtg aaggtaattt gttgataatt agtgctgctg gaaaaggtaa ggataaaaaa
    19501 gggtgacagc aggaaaatga gatggttttg ctttattaac aacgggctaa acgtgtagta
    19561 tttgagttca ctgccgtaca ggcagcttag aaattcactg gtaacatact ccacccgccc
    19621 accatgttca ctgccgtaca ggcagataaa atgcgaaaaa aaagcccgta ctttcgtacg
    19681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    19741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctgcc
    19801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    19861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    19921 cgagacaagt tatggactta tacttccaaa gtacttcatg catatcacaa aataaaaagg
    19981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    20041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    20101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    20161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    20221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    20281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    20341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    20401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    20461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    20521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcatcgtgg tacccagaaa
    20581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatt ccggcgggga
    20641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    20701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    20761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    20821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    20881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    20941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    21001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    21061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    21121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    21181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    21241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    21301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    21361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    21421 tgaccattaa caccgttttc tcacgcatca tttctgcaag caattcaagg atctggcttt
    21481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    21541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    21601 cacctaacca actgttgaga cctgcatcct cgagctgctt ctccagacca acgcgacgta
    21661 agatcaccgc cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    21721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    21781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    21841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    21901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    21961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    22021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    22081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    22141 cggctaacgc gcaaaagaca aacagggcaa ttaacgcgcc gggttgagca ttgccgccaa
    22201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    22261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    22321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    22381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    22441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    22501 taatgccgcc cagcgtaaag gcgagggtgg aatcaaggaa acttaacccg attgtcacca
    22561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    22621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    22681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    22741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    22801 ctgcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    22861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    22921 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    22981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    23041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    23101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    23161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    23221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    23281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    23341 caggcgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    23401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgta
    23461 atgttgctgc cggtaattgt gggttttgac caacccagga gagatgttta cgccatgatt
    23521 ctggtgataa atcgcgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    23581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcgacca accaacactg
    23641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    23701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggttgatgct aattccgtct
    23761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    23821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    23881 ctggcgcaag gatcagggcc agaaaacccg cagccagcgt cacgccagta tcgtagtgac
    23941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    24001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagccgtagc acttccattg
    24061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    24121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    24181 caagagcgag aaagttacgt cggttagcat cggcagcccc catcccaacc agcgccataa
    24241 acaacggaat taacggtgca gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    24301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcaggtagc
    24361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    24421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    24481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    24541 cccatgcgcg cagtacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    24601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccat gcctgggcaa
    24661 tgatcaatat gccgctcaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    24721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    24781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    24841 aggcgaccca tagtcgcatg gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    24901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagcctgt
    24961 accagcagat gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    25021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgcactt tgatgtagcc
    25081 gttttccagt tccagctgcc cttcgaaaat ggcagtattc gggctgtgac cgatagcaac
    25141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    25201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    25261 aatgatgttg ccgttctcca ctttatccat cagacgctta atgaggattt tttccgcgcg
    25321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    25381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    25441 accgtcgcag gttgcacaag cagaaactcc acggccttta aaggcttctt cagagggcag
    25501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    25561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    25621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgctccatta ataacggacc
    25681 ggtcagatcg tttggatcgc caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    25741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg catagacagc
    25801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    25861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    25921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    25981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    26041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    26101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    26161 cttatatgcg cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt
    26221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    26281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    26341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    26401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    26461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    26521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    26581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    26641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    26701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    26761 aacacgcgtg ccggatatgt cagcctaccg taagttgctg ggggaaaccc tgctgcgtct
    26821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    26881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    26941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    27001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    27061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    27121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    27181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    27241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    27301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga aaacattgat
    27361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    27421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    27481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    27541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    27601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    27661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaaccac
    27721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    27781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    27841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    27901 ccggacgacg tcctattttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    27961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    28021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    28081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    28141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    28201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    28261 gcacctgcag ctgaacaacc tgcgcaacag ccgtattatg cccctgcgcc agaacaaccg
    28321 gtggcaggta acgcctggca agccgaagag cagcaatcca cttttgctcc acagtctaca
    28381 taccagactg agcaaactta tcagcagcca gccgctcagg agccgttgta ccaacagccg
    28441 caacccgttg aacagcagcc tgttgtggag cctgaacccg ttgtagaaga gacaaaaccc
    28501 gcgcgtccgc cgctttacta ctttgaagaa gttgaagaga agcgagcccg tgaacgtgaa
    28561 caacttgcgg cctggtatca accgattcca gaaccggtta aagaaccaga accgatcaaa
    28621 tcttcgctga aagcaccttc tgttgcagca gtacctccag tagaagccgc tgccgctgtt
    28681 tctccgctgg catctggcgt gaaaaaagcg acactggcga cgggggctgc cgcaaccgtt
    28741 gccgcgccag tcttcagtct ggcaaatagc ggtggaccgc gtccgcaggt caaagagggg
    28801 attggcccgc agttgccacg accgaaacgt atccgcgtgc caactcgtcg tgaattggcg
    28861 tcttacggta ttaagctgcc ctcacagcgt gcggcggaag aaaaagcccg tgaagcccag
    28921 cgcaatcagt acgattctgg cgatcagtac aacgatgatg aaatcgatgc gatgcagcag
    28981 gatgaactgg cacgtcagtt cgcccagaca cagcagcaac gctatggcga acagtatcaa
    29041 catgatgtgc ccgtaaatgc agaagatgca gatgctgcgg cagaggctga actggctcgt
    29101 cagtttgcgc aaactcaaca acaacgttat tccggcgaac aaccggctgg ggcgaatccg
    29161 ttctcgctgg atgattttga attttcgcca atgaaagcgt tgctggatga tggtccacac
    29221 gaaccgctgt ttacgccaat tgttgaacct gtacagcagc cgcaacaacc ggttgcaccg
    29281 cagcagcagt atcagcagcc acaacagccg gttgcgccac agccgcagta tcagcagcca
    29341 caacagcagg ttgcgccgca gccacaatat cagcagccgc aacaaccggt tgcgccacag
    29401 cagcaatatc agcagccgca acaaccagtt gcgccgcagc cgcagtatca acagccgcaa
    29461 caaccggttg caccgcagcc gcagtatcag cagccacaac agccagttgc gccacagccg
    29521 caggatactc tgcttcatcc gctgttgatg cgtaatggcg acagccgtcc gttgcataaa
    29581 ccgacgacgc cgctgccttc tctggatttg ctgacaccgc cgccgagcga agtggagccg
    29641 gtagatacct ttgcgcttga acaaatggct cgcctggtgg aagcgcgtct ggctgatttc
    29701 cgtattaaag ccgatgtcgt caattactct ccggggccgg ttatcactcg ctttgaactt
    29761 aacctggcac cgggcgtaaa agcggcgcgc atttctaact tgtcacggga ccttgcccgt
    29821 tcactttcga cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt
    29881 ctggagttac cgaataaaaa acgacaaacc gtttatctgc gcgaagtatt ggataacgcc
    29941 aaattccgcg ataatccatc gccattaacc gtggtgctgg gtaaagatat cgccggtgag
    30001 ccggtggttg ccgatctggc gaaaatgccg cacttgttag ttgcggggac taccggttcc
    30061 ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc tttataaagc acagccagaa
    30121 gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc tttcggtgta tgaaggcatt
    30181 ccgcacctgt taacggaagt cgttactgat atgaaagatg ccgccaacgc gctgcgctgg
    30241 tgtgttaacg agatggagcg tcgttataaa ctgatgtctg cgctgggtgt gcgtaatctg
    30301 gcgggttata acgaaaaaat tgctgaagcc gatcgcatga tgcgtccgat tccagacccg
    30361 tactggaagc cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa agaaccatac
    30421 attgtggtgt tggtggacga atttgccgac ctgatgatga cagtaggtaa aaaagtggaa
    30481 gagctgatag cacgtctggc gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca
    30541 actcagcgtc catcggttga tgttattact ggtctgatta aagcgaatat tccgacccgt
    30601 atcgccttta ccgtatccag taagattgac tcacgtacca ttcttgatca ggctggcgcg
    30661 gaatcactgc tgggtatggg ggatatgctc tactctgggc cgaactccac gttgccggta
    30721 cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa
    30781 gcgcgtggtc gcccacagta tgttgatggc atcacctccg acagcgaaag cgaaggtggt
    30841 gcgggtggtt tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt
    30901 gtcactgaaa aacgcaaagc gtcaatttct ggcgtacaac gtcagttccg cattggttat
    30961 aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg ggattgtcag cgaacagggg
    31021 cacaacggta atcgtgaagt gctggcccca ccgccgtttg actaattaat gcattgccgg
    31081 ataaggcgcg gtagcgtcgc atccggcact ctatcaactg aaaattcagt attttcttct
    31141 ttcctcaagc tgattattag cctggaatag agagtagagg gaactcccga tcgggagtga
    31201 cgtaatttga ggaataatga tgaaaaaaat tgccatcacc tgtgcattac tctcaagctt
    31261 agtagcaagc agcgtttggg ctgatgccgc aagcgatctg aaaagccgcc tggataaagt
    31321 cagcagcttc cacgccagct tcacacaaaa agtgactgac ggtagcggcg cggcggtgca
    31381 ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta ttcaactggc atatgacaca
    31441 acctgatgaa agcattctgg tttctgacgg taaaacactg tggttctata acccgttcgt
    31501 tgagcaagct acggcaacct ggctgaaaga tgccaccggt aatacgccgt ttatgctgat
    31561 tgcccgcaac cagtccagcg actggcagca gtacaatatc aaacagaatg gcgatgactt
    31621 tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag ttcaccatta acgtgggacg
    31681 tgatggcaca atccatcagt ttagcgcggt ggagcaggac gatcagcgca gcagttatca
    31741 actgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa tttaccttca ccccgccgca
    31801 aggcgtcacg gtagatgatc aacgtaagta gaggcacctg agtgagcaat ctgtcgctcg
    31861 atttttcgga taatactttt caacctctgg ccgcgcgtat gcggccagaa aatttagcac
    31921 agtatatcgg tcagcaacat ttgctggctg cggggaagcc gttgccgcgc gctatcgaag
    31981 ccgggcattt acattctatg atcctctggg ggccaccggg taccggcaaa acaaccctcg
    32041 ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg tatttctgcc gtcacctctg
    32101 gtgtgaaaga gattcgcgag gcgatcgagc gcgcccggca aaaccgcaat gcaggtcgcc
    32161 gcactattct ttttgttgac gaagttcacc gtttcaacaa aagccagcag gatgcatttc
    32221 tgccacatat tgaagacggc accatcactt ttattggcgc aaccactgaa aacccgtcgt
    32281 ttgagcttaa ttcggcactg ctttcccgtg cccgtgttta tctgttgaaa tccctgagta
    32341 cagaggatat tgagcaagta ctaactcagg cgatggaaga caaaacccgt ggctatggtg
    32401 gtcaggatat tgttctgcca gatgaaacac gacgtgccat tgcagaactg gtgaatggcg
    32461 acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga tatggccgaa gtcgatgata
    32521 gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat cgccggtgaa cgtagcgccc
    32581 gctttgataa caaaggcgat cgcttttacg atctgatttc cgcactgcat aagtcggtac
    32641 gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg aattattacc gctggtggcg
    32701 atccgttata tgtcgcgcgt cgctgtctgg cgattgcgtc tgaagacgtc ggtaatgccg
    32761 atccacgggc gatgcaggtg gcaattgcgg cctgggattg ctttactcgc gttggcccgg
    32821 cggaaggtga acgcgccatt gctcaggcga ttgtttacct ggcctgcgcg ccaaaaagca
    32881 acgctgtcta cactgcgttt aaagccgcgc tggccgatgc tcgcgaacgc ccggattatg
    32941 acgtgccggt tcatttgcgt aatgcgccga cgaaattaat gaaggaaatg ggctacgggc
    33001 aggaatatcg ttacgctcat gatgaagcaa acgcttatgc tgccggtgag gtttacttcc
    33061 cgccggaaat agcacaaaca cgctattatt tcccgacaaa caggggcctt gaaggcaaga
    33121 ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa tagccccata aaacgctacc
    33181 gttaatgtta tcgttgcggt aatgttgtta ctgtatccct gtggtcgcag gctgtggcca
    33241 catctcccat ttaattcgat aagcacagga taagcatgct cgatcccaat ctgctgcgta
    33301 atgagccaga cgcagtcgct gaaaaactgg cacgccgggg ctttaagctg gatgtagata
    33361 agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt caaaacggaa aacctgcaag
    33421 cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa agcgcgcggg gaagatatcg
    33481 agcctttacg tctggaagtg aacaaactgg gcgaagagct ggatgcagca aaagccgagc
    33541 tggatgcttt acaggctgaa attcgcgata tcgcgctgac catccctaac ctgcctgcag
    33601 atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga agtcagccgc tggggtaccc
    33661 cgcgtgagtt tgactttgaa gttcgtgacc acgtgacgct gggtgaaatg cactctggcc
    33721 tcgactttgc agctgcagtt aagctgactg gttcccgctt tgtggtaatg aaagggcaga
    33781 ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga tctgcatacc gaacagcatg
    33841 gctacagtga gaactatgtt ccgtacctgg ttaaccagga cacgctgtac ggtacgggtc
    33901 agctgccgaa atttgctggc gatctgttcc atactcgtcc gctggaagaa gaagcagaca
    33961 ccagtaacta tgcgctgatc ccaactgcag aagttccgct gactaacctg gtgcgcggtg
    34021 aaatcatcga tgaagatgat ctgccaatta agatgaccgc ccacacccca tgcttccgtt
    34081 ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat ccgtatgcac cagttcgaca
    34141 aagttgaaat ggtgcagatc gtgcgcccgg aagactcaat ggcggcgctg gaagagatga
    34201 ctggtcatgc agaaaaagtc ctgcagttgc tgggcctgcc gtaccgtaaa atcatccttt
    34261 gcactggcga catgggcttt ggcgcttgca aaacttacga cctggaagta tggatcccgg
    34321 cacagaacac ctaccgtgag atctcttcct gctccaacgt ttgggatttc caggcacgtc
    34381 gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac ccgtctggtt cataccctga
    34441 acggttctgg tctggctgtt ggtcgtacgc tggttgcagt aatggaaaac tatcagcaag
    34501 ctgatggtcg tattgaagta ccagaagttc tgcgtccgta tatgaacgga ctggaatata
    34561 ttggctaata cccaattttt ctgaatctaa aaagcgccta cgggcgcttt ttttgtctcc
    34621 ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc gagtgaaaat
    34681 ctacctatct ctttgatttt caaattattc gatgtataca agcctatata gcgatctgct
    34741 atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgagcaa gcttgagtga
    34801 gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggtgag tcgccgtggt
    34861 ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt aacattacct
    34921 tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcagg cgaaaaggtt
    34981 atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat gcacgtcgtg
    35041 gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta cgacggcctg
    35101 caccaggttc gcgcctgcct gcgtgggcgt tccatgcgtc gccgtgtcta caatccggac
    35161 cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt cgagcgcatt
    35221 agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat caaagagtac
    35281 ggcaacgaat ccatctatct gaactatggc accggtacgc tgggtggcac catgacccgc
    35341 tcctggccgc cgggaaatac cctggtcgcg cggctgatga actgctgcgg cggctatctg
    35401 aaccattacg gcgactactc ctccgcgcaa attgcggaag gtctgaacta tacctacggc
    35461 ggctgggcag atggcaacag cccgtcggat atcgaaaaca gtaagctggt agtgctgttt
    35521 ggtaataacc ctggcgaaac gcgaatgagt ggcggtgggg tgacttacta tcttgaacag
    35581 gcacgccaga aatctaatgc ccgcatgatc atcatcgatc cgcgctatac cgacaccggt
    35641 gccgggcgcg aagatgagtg gatccctatt cgtccgggaa cagatgccgc actggttaac
    35701 ggtctggcgt acgtcatgat cactgaaaac ctggtggatc aggcattcct cgataaatat
    35761 tgcgttggct acgatgagaa aaccctgcca gccagtgcgc cgaaaaatgg ccactataaa
    35821 gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg ggcctcgcaa
    35881 atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg cagtaccaaa
    35941 ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga aatcgcaacc
    36001 cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg agggaacagc
    36061 ggcgcgcgtg aaggttcata cagtttaccg tttgtccgta tgccgacctt ggaaaacccg
    36121 atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg cccggaaatg
    36181 acggcgctac gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat caaaatgatc
    36241 tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg tacccatgaa
    36301 atccttcagg atgataagaa gtgcgagctg attgtggtta tcgactgcca catgacctca
    36361 tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca gatggacttt
    36421 gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca ggtgattaaa
    36481 ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa acgtcttggc
    36541 gttgagcaac agtttactga aggccgtacc caggaagagt ggatgcggca tctgtatgcc
    36601 cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa gcaggggatc
    36661 tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg tgaagatccg
    36721 caggcaaatc cactgaccac gccatcgggt aaaattgaga tttattcgca ggcgctggct
    36781 gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact gccgatctac
    36841 acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct gcagcttaca
    36901 ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt gctgaaagcg
    36961 gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg tatccacaac
    37021 ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc gaaagtgacg
    37081 ccacgaatga tgccgggtgt ggtcgcactg ggtgaaggtg cctggtatga cccggatgca
    37141 aaacgtgtcg ataagggtgg ttgtattaac gtactgacca ctcaacgtcc gtctcctctc
    37201 gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta aggagtaacc
    37261 gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt gcaaaacctg
    37321 cgagctggct tgtaaagact acaaagattt gatgccagaa gtcagcttcc gccgcattta
    37381 tgaatatgct ggtggcgact ggcaggaaga taacggtgtc tggcaccaga acgtgtttgc
    37441 ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta aagtctgccc
    37501 gagcggtgcg atgcataaac gtgaagatgg ttttgttgtg gtcgatgaag atgtgtgcat
    37561 tggctgccgc tactgccata tggcttgccc gtatggcgca ccgcaatata acgaaacgaa
    37621 aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta aaaagccgat
    37681 ctgtgttgaa tcttgtccgc tgcgggcgct ggatttcggc cctatcgacg agctgcgtaa
    37741 aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta ccaaaccgaa
    37801 tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg gctatctggc
    37861 aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg atgatcttca
    37921 cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg ctgcttaaag
    37981 gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc ttatgggtgc
    38041 tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg cgcgccttta
    38101 actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc ggttcgatct
    38161 tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg tcacctgcat
    38221 tgcgtacgct atggctggtg gtgacgatgg ttcttggcgt cgtctttgtc tggatgatgg
    38281 tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg acgccgatgg
    38341 gcttcttcct gacgatgttc atgggcggcc cgttactggg ttacctgttg ctgagtctgg
    38401 ctggcgtcga tggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg
    38461 tgagtggcgt gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc
    38521 agcaggccgc agcgctggtg ccggactatg gtgcgctgat gtcctggcga atcgtgcttt
    38581 tggccgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc
    38641 cgctactatc cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat
    38701 tctatggttt gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg
    38761 gttcggcccc ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat
    38821 tacgataatc aggaatgtgg ttagagaaca acgtcgccag tccttcaata tcattacgcc
    38881 agtcacggtg cagctcgcaa gccacgccga accatgtcat caattgcgca cctgcttgcg
    38941 acatgcgatc ccatgccgaa tgccgggtaa tttcattaaa ggtccctgac gcgtcagtca
    39001 caacaaagac atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt
    39061 cggttaccac accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa
    39121 aatcttcgtt atcccaggcg ttaatattgc cagggcgagc aatgtaaggt gcatcgggga
    39181 attgtgcttt cagctctgga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa
    39241 gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa
    39301 acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca
    39361 aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc
    39421 gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac
    39481 gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt
    39541 gtgttagatc aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg
    39601 aatgagtaat ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga
    39661 caatgttttt tgcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc
    39721 atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg
    39781 gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca
    39841 tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg
    39901 acagggtatg tcattaaacg cattggcttt aaccgcagct attatctggc ctccttcatt
    39961 tttgccgctg gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg
    40021 cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtag tcgttgagag cgcgctgatg
    40081 tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac
    40141 gtgggaacat ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta
    40201 ttgccgtggg ttacaggttt gacgttggca gggatcttac cgctgttgtt tacgcgtgtc
    40261 ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cgtcaatgct aaaactccgt
    40321 caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgtgctggg atctctatat
    40381 ggcctgatgc cgctgtacct caatcacaaa ggggtgagca atgccagcat tggtttctgg
    40441 atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat
    40501 aagtttggtc gactgctggt gttgcgtgtt caggtctttg tcgtcattct cggcagtatc
    40561 gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt
    40621 acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg
    40681 gcgatgaacc aggccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt
    40741 accgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta
    40801 tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct
    40861 cacgtgtaaa tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt
    40921 tcttctatct gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag
    40981 gttataattt tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt
    41041 ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcaggaaa
    41101 aacagctgcg atggtacaac attgcgctga tgtcttttat cactgtctgg ggttttggca
    41161 acgttgttaa taactatgcc aaccaggggc tggtggttgt tttttcatgg gtgtttatct
    41221 ttgcgctcta tttcacacct tatgcactaa ttgttggtca gttaggctcg accttcaaag
    41281 atgggaaggg cggggtcagt acctggatta aacacacgat gggacccgga ttggcttacc
    41341 tcgccgcgtg gacctactgg gtggtgcata ttccctatct ggcacaaaaa ccccaggcaa
    41401 tactgattgc gctcggttgg gcgatgaaag gcgacggttc gctaatcaaa gaatattcag
    41461 tcgtagcgtt acaggggtta acgctggtgc tgtttatctt ctttatgtgg gttgcttcac
    41521 gcggtatgaa atcgctgaaa atcgtcggtt ctgtggcagg gattgcgatg tttgttatgt
    41581 cgctcctgta tgtggcgatg gcggtaaccg cgcctgcgat tactgaagtg catattgcga
    41641 ccacaaacat tacctgggaa acgttcattc ctcatatcga ctttacctac attaccacta
    41701 tttcaatgct ggttttcgcg gttggcggag ctgagaagat ttctccttac gttaatcaaa
    41761 cgcgcaaccc aggaaaagaa tttccaaaag ggatgttatg cctggcggtg atggttgcgg
    41821 tttgtgccat tctgggctca ctggcgatgg ggatgatgtt tgactcgcgt aatatcccgg
    41881 atgacttaat gactaacggt cagtattacg cctttcagaa gctgggcgag tattacaaca
    41941 tgggtaatac tttaatggtg atttacgcca ttgcgaatac cctgggacaa gtagctgcgc
    42001 tggtattctc gattgatgcc ccgcttaaag tgctattagg cgatgctgac agcaaatata
    42061 ttccagccag tttatgtcgt accaacgctt ctggtacgcc cgttaatggc tattttctga
    42121 ccctggtgct ggtggcgatc ctgattatgc tgccgactct cggcatcggt gatatgaaca
    42181 atctctataa atggctgttg aatcttaact cggtagtgat gccgctgcgt tatttatggg
    42241 tatttgttgc atttattgca gtcgttcgcc tggcgcagaa atatacacca gagtatgtct
    42301 ttattcgtaa taagcctctg gcaatgaccg tcgggatttg gtgttttgcc tttactgcct
    42361 ttgcctgttt gacggggatc ttcccgaaaa tggaagcctt cactgcagag tggaccttcc
    42421 agttggcgct gaatgttgca acgccgtttg tgctggtagg attaggactg atattcccgc
    42481 tgctggcgcg taaagcgaat agtaaataat tattgtggtc ggtgacgctg actctggcag
    42541 atctgacccg tttttacata atcgagaaat gcgcgcaagc cgcaggacat atactggcgg
    42601 tttgggtaat acatttggaa gctgtgctgt aagggcgtta gaaatagagc tctgaattat
    42661 ttatgcagcg cggaataaaa aagcggcaac tcaataaagt tgccgcttta cggggaaatt
    42721 agaacattac cttatgaccg tactgctcaa gaataccttt cacgcgttcc atggtttctt
    42781 tcttcggtgg tttaacacca tcgagtttgt attcttcacc catcgccacc catttgtgtt
    42841 tgcccagttc gtggtagggg agaagttcga ttttctcaac gttacccata tcacgggtaa
    42901 attcaccgag gcgatgcgct gaatcgtcat cgtcagacca gcctgggaca acaacgtagc
    42961 ggatccacac cttcacattt ttattcgcca gatatttggc gaactccagc gtgcggtggt
    43021 tggaaactcc aaccagattt tggtggatct cgtcgttcat ctgtttgaga tcgagcatta
    43081 ccaggtcggt tacttccagc agttcatcaa tcaccggatc gtaacgacga acaaaaccgt
    43141 tggtgtccag acaggtatga atgccttctt ttttgcaggc gcggaaccag tcacgaacaa
    43201 actcagcttg caggattgct tcaccgccgg atgcggtaac gccgccgccg gaagcgttca
    43261 taaagtggcg ataggtcacc acttccttca tcaaatcttc aacggtaact tctttaccgc
    43321 catgcgtgtc ccaggtgtcg cggttatgac aatacaggca gcgcatcagg cagccctgga
    43381 aaaaggtgat aaagcgaata cctgggccgt ctacggttcc acaggattca aaggagtgaa
    43441 tgcgaccaat aactgacatt gcggtgtttc tccagatgtg gcccatctga ggccgtgttg
    43501 gtgcgcagct cgaaggctac gtcgagtctg ttttggcagt caccttaaag tatagatagc
    43561 tgacaaaaaa ggctcacgtg ctaaaaaagg ccccactttc gtggagcctt tattgtacgc
    43621 tttttactgt acgatttcag tcaaatctaa ttacatagat tgagtgaagg tacgagtaat
    43681 aacgtcctgc tgctgttctt tagtcagcga gttgaaacgt actgcgtagc cagatacacg
    43741 gatggtcagc tgcggatatt tttccggatt ttccatcgcg tcgagcagca tttcacggtt
    43801 catcacgtta acgttcaggt gctgaccacc ttcgatggat gcttcgtggt ggaagtaacc
    43861 atccatcaga ccagccaggt tggtcttacg aacttcgtcg tctttaccca gtgcgttcgg
    43921 aacgatagag aaggtgtagg agataccatc tttagcgtaa gcaaacggca gtttagcaac
    43981 ggaagtcaga gacgctacag cacctttctg gtcacgaccg tgcatcgggt tagcacccgg
    44041 tccgaacggc gcgccagcac gacgaccgtc tggggtgtta cccgttttct taccatacac
    44101 aacgttagaa gtgatggtca gaacagactg agtcgggata gcgtcacggt aggtgtgcag
    44161 tttctgaatt ttcttcatga aacgttctac caggtcaaca gccaggtcat ctacacgcgg
    44221 atcgttgtta ccaaactgcg ggtattcgcc ttcgatttcg aagtcgatag ccagaccgtc
    44281 ttcgtcacga atcggtttaa ctttcgcata tttgattgca gacagggagt cagcagcaac
    44341 ggacagacca gcgataccac acgccatggt gcggataacg tcacggtcgt gcagcgccat
    44401 cagagaggct tcgtagctgt acttgtcgtg catgtagtga atgatgttca gtgcagtgat
    44461 gtactgttta gccagccagt ccatgaagtg atccatgcgc tccatcactt catcgtagtt
    44521 caggagatcg cctttgatcg gttcagactt cggaccaacc tgcattttca gtttttcgtc
    44581 aacgccgccg ttgattgcgt acagcatggt tttcgccagg tttgcacgcg caccgaagaa
    44641 ctgcatttgt ttaccaacga tcatcgggct tacgcagcaa gcgatagcgt agtcatcgtt
    44701 gttgaagtcc ggacgcatca ggtcatcgtt ctcatactgc agagaagagg tgtcgatgga
    44761 cactttagcg gcgaatttct tgaagttcag cggcagtttt tcagaccaca gaatggtcat
    44821 gttcggttcc ggagacggac ccatggtgta cagggtgttc aggaaacgga agctgttttt
    44881 ggtaaccagg gtacgaccgt cgaggcccat accaccgata gattcggttg cccagatcgg
    44941 gtcgccagag aacagttcat cgtactccgg agtacgcagg aagcgaacca tacgcagttt
    45001 catgaccagg tggtcaacca tttcctgcgc ttcttgttcg gtgatcttgc cagctttcag
    45061 gtcacgttcg atgtacacat ccaggaaggt ggaggtacga ccgaaggaca ttgcagcacc
    45121 gttctgagac ttaacagcag ccaggtagcc gaagtaagtc cactggatag cttcctgagc
    45181 gttggtagcc ggaccagaga tgtcgtagcc gtatttcgca gccatttctt tcatctgacc
    45241 cagagcgcgg tgctgttcag cgatttcttc gcgcagacgg atagtctgtt ccaggtttac
    45301 gccgttttcc agatcagcct gcagagaggt gaactgagcg tatttgtctt tcatcaggta
    45361 gtcgataccg tacagcgcaa cgcgacggta gtcaccgatg atacggccac ggccataagc
    45421 atctggcaga ccggtcagaa caccggattt acggcaacgc aggatgtccg gagtgtaaac
    45481 gtcgaacacg ccctggttgt gagttttacg gtattcagtg aagatttttt tgatcatcgg
    45541 gtccagttcg cggttgtacg ctttgcagga accttcgatc attttgatac caccgaacgg
    45601 gataagagca cgtttcagcg gagcttcagt ctgcagacca acaacttttt ccaacgcttt
    45661 gttgatgtag ccagcgtcgt gagaggtgat ggtggaagca acagcggtgt cgaagtcaac
    45721 tggcgcgtga gtgcggtttt ccagtttaac gccttccatt actttgtccc acagggtggt
    45781 ggtcgcttca gtagcgccag ccaggaagga ctcgtcaccc tcgtacggag tgtagttttt
    45841 ctgaatgaag tcacggacgt ttacttcatt ctgccagtca cctttggtaa aaccttccca
    45901 ggctgtggct aacttttcat taagctcgga catgtaacac ctaccttctt aagtggattt
    45961 tttatttact gcgtacttcg acaaccatta atggtgatcg ttttcacgca ggtaaatgac
    46021 ccagtatgtc aacccaacca acaaaccacc gccgataata ttaccgatcg taaccggaat
    46081 caggttatca gtgatgaagt tcatcacggt caggtgagaa aaattttccg gtgcagaacc
    46141 gacagcggtc cagaattccg gagatgcgaa gtcgcggatt acaataccca tcgggatcat
    46201 aaacatgttt gcgatactgt gctcaaaacc gctggcaaca aacatcgcga ccggcagcac
    46261 catgataaac gctttgtcca tcaggctgcg gccagaataa ctcatccata ctgccagaca
    46321 taccatcagg tttgccagga taccaaggca gacggcctca ataaaagtat ggtgcacttt
    46381 gtggtcggcg gtttgtagga cgtttagtcc ccattgacca tttgcggtca tatactcgcc
    46441 ggaaagccac attaaaagta caaacagcag tgcgccgacc aggttgccaa aatagacatt
    46501 tagccagttt ttagccaact gaccccaggt gatgcgccca ctcgccttag caacaacaat
    46561 caacactgtg gaagtaaaga gatcggctcc gcagacaaca caaagaatca gtcccagaga
    46621 gaagcaaatg ccgccaacca gttttgccat gccgaagggc attgtgcctg tgccagtggt
    46681 tgctgtgata tagaagacga atgcgattga gatgaaaaca ccggcggtaa tcgccagata
    46741 gaaagtctta agcggatgtt tcgttgcttt atagacaccc gcctcttcgg ccactttggc
    46801 cattgcagca ggaagtaaaa gatcaaaagg gttgtcagct ttcacactaa ctctctcttt
    46861 attaagtcgg cgacgagata ctaacaaagc attatagatg agaaattgat atagatcata
    46921 tctcgcctgg cttataggcc cgtaactcgc atggttttta tgcaaatacg gagtaaatat
    46981 ttgattatcc aaataaaaat aaattttaaa aattaacaaa tgagttgaat tttttccgca
    47041 tcctccgcta aaacagttaa ttaaaaggga gcatcaggcg aataaagtaa caatatcgat
    47101 cgtatttatt aaatacaaat taccgatatt taaccttata attacaatta ttttattaat
    47161 gcaaatatat gtaaagcggg gcattaaaaa aacgccccgt aatataactc agacgaatca
    47221 ttaagcctac attacgtagg ctatttgatt ttattttgcc cagaatgctg ctttggcgcg
    47281 ctgcagcttt tcgtaggcct tcaacagcga ctgatgtgca gcaaacgcgt gcagatcgct
    47341 atctactggt tgcaagccat aaaacgccgc ttcgccgctc attgccgcac tggcggcttc
    47401 cacggcatct gcgccgtaca tgcgaacaaa cgcattcaga tattgcagcg gctggcgatc
    47461 ttcttcctgc gccagtaata acaacgtttg caggcagcga taatagttgg cgcgttccgg
    47521 gctgaatacc gatgagttaa actccatcgt ccattcggtc cagaccagag cctgttccag
    47581 atcgccacca gccagcgcca gcatggcttt taattcacca atacgcaggg tgtaccaacc
    47641 gttatccgac ccggtcgcca gacccaacag ctcacgcacg cgggtaaagt catcaaaacc
    47701 ttcttcatcc agttgctcga tgaggttcag gtaatcttct ttttcccact cgctgcctgg
    47761 tagcgaaaga atcgtttcac gtaaatggct gcccatactg ttattcgcga gccacagatc
    47821 ttcagccgga taaatatcgg acatgccagg cacgataata cggcaagcat aaacgcccag
    47881 atgctcgtaa tcggcaatat aaacttcttt atcttctttg ttgaagatag ccatcagcgt
    47941 agcaaactct tcttccgtgg tgccggagaa attccagtcc acaaacggat aatcggcatc
    48001 ctgcttgaac aggtcccagg agattaaacc gctggaatcg ataaagtgcg tttcgaggtt
    48061 ggtatgttca gcgacttctt catcatcgaa ggttggcgga gtaaacacat ccaaatcttt
    48121 caggccacga ccttgcagca gctcggtcac ggtacgttcc agtgctacgc caaaatcagg
    48181 atgcgcaccg aaagaggcaa agcaggtgcc gttagcagga ttaaacagta ccacgcaaat
    48241 caccggatac tggccgccaa gggaaccatc atatgcgaag atcgggaaac cttctgcttc
    48301 cagtgtttcg atcgcttcaa ccaccgctgg gtaacgcgcc agcacgtctg ccgggatttc
    48361 cggcaagctg atgctttcag caataatgcg gtttttcacg tagcgttcga aaacttcgga
    48421 caacccctga acgcgtgctt cgttacgggt attgccagca gacataccgt tagaaacgta
    48481 caggttacca atgatattca tcggaatata aacggtctga ttatcagact gacgtgtaaa
    48541 cggcagaccg caaataccac gatcttcgtt accggattgt aggtcaatca gcatactgcc
    48601 ggtcaattca ttctccggat cgtaaaatgc gcgcagacgt tcatcgagca gcccttctgg
    48661 cacatcgtca ttttcggtca gtgggaacca tttttcgttg ggataatgca caaatggacc
    48721 gttggcgatg gtttcgccca gccagaaatc agcaaaaaag tagttggttg agagacgctc
    48781 gaaatattca ccgagtgcag aagccagcgc cgctttcttg gttgcgcctt taccgttggt
    48841 aaaacacagt gcgcactctt tgtcgcgaat atgtacagac cagacgttag gcacgggatt
    48901 cagccaggag gcctcttcaa tctgaaagcc gaggtctgaa agtttttgct ggaagcgagc
    48961 gatggaatct tccagtgcgg catctttgcc ggggataaat gtttgcgtca tgaaaatcac
    49021 tttagtcgta cggaaagcgc gcaataatac gggttttatc tcaaaggcgc tatcaccgcc
    49081 gccatgccga tgaactgttg actatgcttt tagcggataa accacgttaa gagcataaaa
    49141 atgaaggcgt tcgatctcca ccgtatggca tttgataaag tgccttttga tttccttggc
    49201 gaagttgcac tacgtagtct ttataccttt gtactggtct ttttgttcct caaaatgacc
    49261 ggaagacgcg gtgtgcggca gatgtcgctg tttgaagttt taatcattct gacgctggga
    49321 tcagcggcgg gagatgtggc gttttatgat gatgtgccga tggtcccggt acttatcgtc
    49381 tttattactc tggcgttgtt ataccgcctg gtaatgtggt tgatggcgca cagtgaaaaa
    49441 ctggaagatc ttctggaagg caagccagtt gtcattattg aagatggcga gctggcctgg
    49501 tcgaaactca ataactccaa catgacggaa tttgagttct ttatggagct acgattgcgt
    49561 ggcgtggagc agctggggca ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt
    49621 gtctatttct ttgaagatga caaggtgaaa tcgggtttac ttattttacc cagtgattgt
    49681 acgcagcgtt acaaagtggt gccggagtcg gcggactatg cctgtattcg ttgtagtgaa
    49741 atcattcata tgaaagcggg ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg
    49801 acgaaggcaa gtcgggcaaa acgggtgacc tgacagtaaa aacatcggct ttttgctaat
    49861 aatccgagag attcttttgt gtgatgcaag ccacattttt gccctcaacg gttttactca
    49921 ttgcgatgtg tgtcactgaa tgataaaacc gatagccaca ggaataatgt attacctgtg
    49981 gtcgcaatcg attgaccgcg ggttaatagc aacgcaacgt ggtgagggga aatggctcaa
    50041 atcttcaatt ttagttctgg tccggcaatg ctaccggtag aggtgcttaa acaggctcaa
    50101 caggaactgc gcgactggaa cggtcttggt acgtcggtga tggaagtgag tcaccgtggc
    50161 aaagagttca ttcaggttgc agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc
    50221 ccctccaact acaaggtatt attctgccat ggcggtggtc gcggtcagtt tgctgcggta
    50281 ccgctgaata ttctcggtga taaaaccacc gcagattatg ttgatgccgg ttactgggcg
    50341 gcaagtgcca ttaaagaagc gaaaaaatac tgcacgccta atgtctttga cgccaaagtg
    50401 actgttgatg gtctgcgcgc ggttaagcca atgcgtgaat ggcaactctc tgataatgct
    50461 gcttatatgc attactgccc gaatgaaacc atcgacggta tcgccatcga cgaaacgcca
    50521 gacttcggca aagatgtggt ggtcgccgcc gacttctctt caaccattct ttcccgtccg
    50581 attgacgtca gccgttatgg tgtaatttac gctggcgcgc agaaaaatat cggcccggca
    50641 ggcctgacaa tcgtcatcgt ccgtgaagat ttgctgggca aagcgaatgt cgcgtgtccg
    50701 tcgattctgg attattccat cctcaacgat aacggctcca tgtttaacac gccgccgaca
    50761 tttgcctggt atctatctgg tctggtcttt aaatggctga aagcgaacgg cggtgtagct
    50821 gaaatggata aaatcaatca gcaaaaagca gaactgctat atggggtgat tgataacagc
    50881 gatttctacc gcaatgacgt ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag
    50941 ttggcggaca gtgcgcttga caaattgttc cttgaagagt cttttgctgc aggccttcat
    51001 gcactgaaag gtcaccgtgt ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg
    51061 ctggaaggcg ttaaagcgct gacagacttc atggttgagt tcgaacgccg tcacggttaa
    51121 tgccgaaatt ttgcttaatc cccacagcca gcctgtgggg tttttatttc tgttgtagag
    51181 agttgagttc atggaatccc tgacgttaca acccatcgct cgtgtcgatg gcactattaa
    51241 tctgcccggt tccaagagcg tttctaaccg cgctttattg ctggcggcat tagcacacgg
    51301 caaaacagta ttaaccaatc tgctggatag cgatgacgtg cgccatatgc tgaatgcatt
    51361 aacagcgtta ggggtaagct atacgctttc agccgatcgt acacgttgcg aaattatcgg
    51421 taacggcggt ccattacacg cagaaggtgc cctggagttg ttcctcggta acgccggaac
    51481 ggcaatgcgt ccgctggcgg cagctctttg tctgggtagc aatgatattg tgctgaccgg
    51541 tgagccgcgt atgaaagagc gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg
    51601 ggcgaagatc acttacctgg aacaagaaaa ttatccgccg ttgcgtttac agggcggctt
    51661 tactggcggc aacgttgacg ttgatggctc cgtttccagc caattcctca ccgcactgtt
    51721 aatgactgcg cctcttgcgc cggaagatac ggtgattcgt attaaaggcg atctggtttc
    51781 taaaccttat atcgacatca cactcaatct gatgaagacg tttggtgttg aaattgaaaa
    51841 tcagcactat caacaatttg tcgtaaaagg cgggcagtct tatcagtctc cgggtactta
    51901 tttggtcgaa ggcgatgcat cttcggcttc ttactttctg gcagcagcag caatcaaagg
    51961 cggcactgta aaagtgaccg gtattggacg taacagtatg cagggtgata ttcgctttgc
    52021 tgatgtgctg gaaaaaatgg gcgcgaccat ttgctggggc gatgattata tttcctgcac
    52081 gcgtggtgaa ctgaacgcta ttgatatgga tatgaaccat attcctgatg cggcgatgac
    52141 cattgctacg gcggcgttat ttgcaaaagg caccaccacg ctgcgcaata tctataactg
    52201 gcgtgttaaa gaaaccgatc gcctgtttgc gatggcaaca gaactgcgta aagtcggtgc
    52261 ggaagtagaa gaggggcacg attacattcg tatcactcca ccggaaaaac tgaattttgc
    52321 cgagatcgcg acatacaatg atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc
    52381 agatacacca gtgacgattc ttgatcccaa atgtacggcc aaaacatttc cggattattt
    52441 cgagcagctg gcgcggatta gccaggcagc ctgaatgaat aacgtccaat aaatagccaa
    52501 aactttcttt atcaaaacgt cggcacattg ccggcgtttt ttttcggacc ttgtgagtca
    52561 ttttgattaa tggtagcgtc gcttgtcaat gtaagttgtt gatacataat gtttatatat
    52621 gattaatcaa cggatgattc acatgaagaa tactaaatta ctgctggcga ttgtgacctc
    52681 tgcagcatta ctgacggggt gtcaaaatac ccacggtatt gataccaata tggctatcag
    52741 ctccggttta aatgcctata aagcagcaac gttaagcgat gccgatgcaa aagcgattgc
    52801 caatcagggc tgtgccgaaa tggacagcgg caatcaagtc gcaagtaaat ccagcaagta
    52861 cggtaaacgt ctggcaaaaa tcgccaaagc attgggtaac aatattaatg gcacgccggt
    52921 caactataag gtttatatga ccagcgacgt caacgcatgg gcgatggcga acggctgtgt
    52981 tcgtgtttac agtggcctga tggacatgat gaacgataac gaaattgaag gcgttctggg
    53041 ccatgaactg ggccacgtcg cgttgggtca ctcgctggct gaaatgaaag cttcttatgc
    53101 gatcgttgcc gcacgcgatg ccatttcagc taccagcggt gtggcttccc agctttcccg
    53161 ctcacaattg ggtgatatcg cagaaggcgc tatcaatgcg aaatactccc gcgataaaga
    53221 gtccgaagca gatgatttct cctttgatct gttgaagaaa cgtggcatca gcacccaggg
    53281 gctggttggc agctttgaaa aactggctag cctggatggc ggtcgcaccc agtccatgtt
    53341 tgactctcac ccaccatcaa cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg
    53401 taagtaaatc attgtcatct ttcgggctgg tcttctgcca gcccgctata attgcgcaat
    53461 aaatccccat ctgaatacag acaaaactgg tttttgcaca caacgttaac gatttgtggc
    53521 gtcggcgcgt ataatgcgcg cggttatgtt aacggtacgc ctgttttaag gagataaaga
    53581 tgacggcaat tgccccggtt attaccattg atggcccaag cggtgcaggg aaaggcacct
    53641 tgtgtaaggc tatggcggaa gcgttgcaat ggcatctgct ggattcgggt gcaatttatc
    53701 gcgtactggc actagcggca ttacatcacc atgttgatgt tgcatcggaa gatgcgctgg
    53761 tgccgctggc atcccatctg gatgtacgtt ttgtgtcaac caatggcaat ctggaagtga
    53821 tcctcgaagg ggaagatgtc agcggcgaaa ttcgtactca ggaagtggcg aatgcagctt
    53881 cacaagtcgc ggcattccca cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc
    53941 gcgaattacc aggcctgatt gccgatggcc gcgacatggg aacggtggta ttccctgatg
    54001 cgccagtgaa aattttcctt gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac
    54061 agttgcagga gaagggcttt agtgttaact ttgagcgcct tttggccgag atcaaagaac
    54121 gcgacgaccg cgatcgtaac cgagcggtag cgccactggt tccggcagcc gatgctttag
    54181 tgttggattc caccacctta agcattgagc aagtgattga aaaagcgcta caatacgcgc
    54241 gccagaaatt ggctctcgca taagcgaccg aatttgcagt acccccgttg caatggaatg
    54301 acagcgggta tgttaaacaa ccccatccgg catggagcca ggtggacgtt aaatataaac
    54361 ctgaagatta aacatgactg aatcttttgc tcaactcttt gaagagtcct taaaagaaat
    54421 cgaaacccgc ccgggttcta tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt
    54481 actggttgac gctggtctga aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc
    54541 ccagggcgag ctggaaatcc aggtaggtga cgaagttgac gttgctctgg acgcagtaga
    54601 agacggcttc ggtgaaactc tgctgtcccg tgagaaagct aaacgtcacg aagcctggat
    54661 cacgctggaa aaagcttacg aagatgctga aactgttacc ggtgttatca acggcaaagt
    54721 taagggcggc ttcactgttg agctgaacgg tattcgtgcg ttcctgccag gttctctggt
    54781 agacgttcgt ccggtgcgtg acactctgca cctggaaggc aaagagcttg aattcaaagt
    54841 aatcaagctg gatcagaagc gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc
    54901 cgaaaacagc gcagagcgcg atcagctgct ggaaaacctg caggaaggca tggaagttaa
    54961 aggtatcgtt aagaacctca ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg
    55021 cctgctgcac atcactgaca tggcctggaa acgcgttaag catccgagcg aaatcgtcaa
    55081 cgtgggcgac gaaatcactg ttaaagtgct gaagttcgac cgcgaacgta cccgtgtatc
    55141 cctgggcctg aaacagctgg gcgaagatcc gtgggtagct atcgctaaac gttatccgga
    55201 aggtaccaaa ctgactggtc gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat
    55261 cgaagaaggc gttgaaggcc tggtacacgt ttccgaaatg gattggacca acaaaaacat
    55321 ccacccgtcc aaagttgtta acgttggcga tgtagtggaa gttatggttc tggatatcga
    55381 cgaagaacgt cgtcgtatct ccctgggtct gaaacagtgc aaagctaacc cgtggcagca
    55441 gttcgcggaa acccacaaca agggcgaccg tgttgaaggt aaaatcaagt ctatcactga
    55501 cttcggtatc ttcatcggcc tggacggcgg catcgacggc ctggttcacc tgtctgacat
    55561 ctcctggaac gttgcaggcg aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc
    55621 tgcagttgtt ctgcaggttg acgcagaacg tgaacgtatc tccctgggcg ttaaacagct
    55681 cgcagaagat ccgttcaaca actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg
    55741 taaagtaact gcagttgacg ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg
    55801 ttacctgcgt gcttctgaag catcccgtga ccgcgttgaa gacgctaccc tggttctgag
    55861 cgttggcgac gaagttgaag ctaaattcac cggcgttgat cgtaaaaacc gtgcaatcag
    55921 cctgtctgtt cgtgcgaaag acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa
    55981 acaggaagat gcaaacttct ccaacaacgc aatggctgaa gctttcaaag cagctaaagg
    56041 cgagtaattc tctgactctt cgggattttt attctgaagt ttgttgagtt tacttgacag
    56101 attgcaggtt tcgtcctgta atcaagcact aagggcggct acggccgccc ttaatcaatg
    56161 cagcaacagc agccgcttaa tttgccttta aggaaccgga ggaatcatga ccaagtcaga
    56221 attgatagaa agacttgcca cccagcaatc gcacattccc gccaagacgg ttgaagatgc
    56281 agtaaaagag atgctggagc atatggcctc gactcttgcg cagggcgagc gtattgaaat
    56341 ccgcggtttc ggcagtttct ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa
    56401 gactggcgat aaagtagaac tggaaggaaa atacgttcct cactttaaac ctggtaaaga
    56461 actgcgcgat cgcgccaata tttacggtta agtttttact caaacttgaa cgagagaaaa
    56521 gcacctgtcg ggtgcttttt tcatttatct aatctggaac tggaagctgc ctcgcagagt
    56581 tttgagcagt tttcaccctt tcgttaaatt cttctgaatg tgcctcgggg aacgcaaaat
    56641 tcccaccaga caaccgctca acaaagttgc acactttcca taaacaggga ggggtgcatg
    56701 aaaataacga cagtcggcgt atgcataatt tgcggaattt ttccgttgct gattttgccc
    56761 caattgcctg ggacattaac ccttgcgttt ctgactctct tcgcctgcgt actggcattt
    56821 atccctgtta aaaccgtccg ttatatcgcg ctgacgttgc tgtttttcgt ttggggcata
    56881 ttatcagcaa agcaaatttt gttggcagga gaaaccttaa ctggcgcgac gcaggatgca
    56941 attgttgaga tcactgctac tgacggcatg accactcatt acggtcaaat tacttatcta
    57001 caaggtcaac gtatattccc tgcgccaggc cttgtgctgt atggcgaata tcttccgcaa
    57061 gcggtttgtg ccggacaact gtggtcaatg aaactcaaag ttcgtgccgt tcatggtcaa
    57121 cttaatgatg gcggctttga tagccagcgt tatgccattg cccagcatca gccgctcacc
    57181 ggccgctttc tgcaggcaag tgttattgaa ccgaattgta gcctgcgtgc acagtatctg
    57241 gcgtcactac aaacaacgct gcaaccctat ccgtggaatg cggttattct tggtttaggt
    57301 atgggggaac ggttatccgt ccctaaagaa atcaaaaata tcatgcgtga tactggaacg
    57361 gcgcatttaa tggcgatatc gggattgcac atcgcttttg cggcgttgct ggctgccgga
    57421 ctcattcgca gtggacaaat ttttctgcct gggcgctgga tccactggca aatgccatta
    57481 attggcggaa tctgctgtgc tgctttttat gcctggctga cgggaatgca acctcctgca
    57541 ttgcgtaccg ttgtggcgct tgctacgtgg ggtatgctta agttaagtgg gcgacagtgg
    57601 agtggctggg atgtatggat atgttgtctg gcggcaattt tgctgatgga tcctgttgtc
    57661 attctctcgc aaagtttatg gctctctgcc gctgcggtcg cggcattgat attttggtat
    57721 cagtggtttc cctgtcctga gtggcaactg ccgccggtat tgcgtgcagt tgtttccctc
    57781 atccatctgc aactgggaat cacacttctg cttatgcccg tgcaaatcgt catttttcat
    57841 ggcattagtc tgacctcgtt tattgcaaat ctattagcaa ttcccttggt gacatttatc
    57901 acggttccgt tgatcctcgc cgcgatggtt gtgcatttaa gcgggccgtt aatcctggat
    57961 caagggttat ggtttcttgc cgaccggtct ttggctttac ttttcttggg gttaaagagt
    58021 ttgccagaag ggtggatcaa cattgctgaa cgttggcaat ggctatcatt ttccccatgg
    58081 ttcttactgg tggtatggcg attaaacgcc tggcgaacgt tgccagcaat gtgtgtggct
    58141 gtaggcttgc tgatgtgctg gccgctgtgg caaaaacctc gacctgacga gtggcaagtg
    58201 tacatgcttg atgtcgggca agggctggca atggtgatag ccagaaacgg caaagcgatt
    58261 ctctatgaca cgggactggc ctggcctgaa ggggatagtg ggcaacaact gattatcccc
    58321 tggctccact ggcataatct tgaaccggaa ggcgttatcc tgagtcatga acatctggat
    58381 caccggggag ggctggactc aatattgcat acatggccga tgttatggat cagaagtccg
    58441 ttaaactggg aacatcatca gccctgtgtg cgtggcgaag cgtggcaatg gcaaggattg
    58501 cgtttcagcg cgcactggcc tttacaaggt cgcaacgata aaggaaataa ccattcctgt
    58561 gtggttaagg ttgatgacgg gacgaatagc attcttctaa ccggtgatat tgaagcccca
    58621 gctgaacaaa agatgctaag ccgttactgg cagcaagtgc aggcaacatt gcttcaggta
    58681 cctcaccatg gcagtaatac ctcatcatca ttgccattaa ttcagcgagt gaatggaaaa
    58741 gtggcactcg catcggcatc gcgctataac gcatggcgac tgccctctaa caaagttaag
    58801 catcgctatc aacaacaagg ttatacgtgg cttgatactc ctcatcaggg gcaagtaacg
    58861 gtcgattttt cagcgcaagg ctggcggatt agcagcctca gggagcaaat tttacctcgt
    58921 tggtatcatc agtggtttgg cgtgccagtg gataacgggt agaatatgcg gctatttcaa
    58981 caaatgctgg ttttttgaat gcataacgac aaagatctct ctacgtggca gacattccgc
    59041 cgactgtggc caaccattgc gccttttaaa gcgggtctga tcgtggcggg cgtagcgtta
    59101 atcctcaacg cagccagcga taccttcatg ttatcgctcc ttaagccact tcttgatgat
    59161 ggctttggta aaacagatcg ctccgtgctg gtgtggatgc cgctggtggt gatcgggctg
    59221 atgattttgc gtggtatcac cagctatgtc tccagctact gtatctcctg ggtatcagga
    59281 aaggtggtaa tgaccatgcg tcgccgcctg tttggtcaca tgatgggaat gccggtttca
    59341 ttctttgaca aacagtcaac gggtacgctg ttgtcacgta tcacctacga ttccgaacag
    59401 gttgcttctt cctcttccgg cgcactgatt actgttgtgc gtgaaggtgc gtcgatcatc
    59461 ggcctgttca tcatgatgtt ctattacagt tggcaattgt cgatcatttt gattgtgctg
    59521 gcaccgattg tttcgattgc gattcgcgta gtatcgaagc gttttcgcaa catcagtaaa
    59581 aacatgcaga acaccatggg gcaggtgacc accagcgctg aacaaatgct gaaaggccat
    59641 aaagaagtat tgattttcgg tggtcaggaa gtggaaacga aacgcttcga taaagtcagc
    59701 aaccgaatgc gtcttcaggg gatgaaaatg gtttcagcct cttccatctc tgatccgatc
    59761 attcagctga tcgcctcttt ggcgctggcg tttgttctgt atgcggcgag cttcccaagt
    59821 gtcatggata gcctgactgc cggtacgatt accgttgttt tctcttcaat gattgcactg
    59881 atgcgtccgc tgaaatcgct gactaacgtt aacgcccagt tccagcgcgg tatggcggct
    59941 tgtcagacac tgtttaccat tctggacagt gagcaggaga aagacgaagg taagcgcgtg
    60001 atcgagcgtg cgactggcga cgtggaattc cgcaacgtca cctttactta tccgggacgt
    60061 gacgtacccg cattgcgtaa catcaacctg aaaattccgg cagggaaaac ggtcgctctg
    60121 gttggacgct ctggttcagg taaatcaacc atcgccagcc tgatcacgcg tttttacgat
    60181 attgatgaag gcgaaatcct gatggatggt cacgatctgc gcgagtatac cctggcgtcg
    60241 ttgcgtaacc aggttgctct ggtgtcgcag aatgtccatc tgtttaacga tacggttgct
    60301 aacaacattg cttacgcacg aactgaacag tacagccgtg agcaaattga agaagcggcg
    60361 cgtatggcct acgccatgga cttcatcaat aagatggata acggtctcga tacagtgatt
    60421 ggtgaaaacg gcgtactgct ctctggcggt cagcgtcagc gtattgctat cgctcgagcc
    60481 ttgttgcgtg atagcccgat tctgattctg gatgaagcta cctcggctct ggataccgaa
    60541 tccgaacgtg cgattcaggc ggcactggat gagttgcaga aaaaccgtac ctctctggtg
    60601 attgcccacc gcttgtctac cattgaaaag gcagacgaaa tcgtggtcgt cgaggatggt
    60661 gtcattgtgg aacgcggtac gcataacgat ttgcttgagc accgcggcgt ttacgcgcaa
    60721 cttcacaaaa tgcagtttgg ccaatgatcg aaaaaatctg gtctggtgaa tcccctttgt
    60781 ggcggctatt gctgccactc tcctggttgt atggcctggt gagtggcgcg atccgtcttt
    60841 gctataaact aaaactgaag cgcgcctggc gtgcccccgt accggttgtc gtggttggta
    60901 atctcaccgc aggcggcaac ggaaaaaccc cggtcgttgt ctggctggtg gaacagttgc
    60961 aacagcgcgg tattcgcgtg ggggtcgtat cgcggggata tggtggtaag gctgaatctt
    61021 atccgctgtt attgtcggca gataccacca cagcacaggc gggtgatgaa cctgtgttga
    61081 tttatcaacg cactgatgcg cctgttgcgg tttctcccgt tcgttctgat gcggtaaaag
    61141 ccattctggc gcaacaccct gatgtgcaga tcatcgtaac cgacgacggt ttacagcatt
    61201 accgtctggc gcgtgatgtg gaaattgtcg ttattgatgg tgtgcgtcgc tttggcaatg
    61261 gctggtggtt gccggcgggg ccaatgcgtg agcgagcggg gcgcttaaag tcagttgatg
    61321 cggtaatcgt caacggcggt gtcccccgca gcggtgaaat ccccatgcat ctgctgccgg
    61381 gtcaggcggt gaatttacgt accggtacgc gttgtgacgt tgctcagctt gaacatgtgg
    61441 tggcgatggc agggattggg catccgccgc gcttttttgc cacgctgaag atgtgcggcg
    61501 tacaaccgga aaaatgtgta ccgctggccg atcatcagtc tttgaaccat gcggatgtca
    61561 gcgcgttggt aagcaccggg caaacgctgg taatgactga aaaagatgcg gtgaaatgcc
    61621 gggcctttgc agaagaaaat tggtggtatt tgcccgttga cgcacagctt tcaggtgatg
    61681 aaccagcgaa actgcttgcg caactaacct cgctggcttc tggcaactag ttacgccgcg
    61741 gcagcgttcg attgatggag tcatgaatgt cgctgccgca cctctccctt gctgatgcgc
    61801 gtaatcttca ccttgccgcg caaggcctgt taaacaaacc ccgccgtcga gcgtcgttgg
    61861 aggatattcc ggcaacgatc tcccgcatgt ccttgctgca aatcgatacc atcaatattg
    61921 ttgcccgtag cccatatctg gtgcttttca gtcgtctggg aaattatcct gcccagtggc
    61981 tggatgagtc tctggcgcgt ggcgaattaa tggaatactg ggcgcatgaa gcctgcttta
    62041 tgccacgcag tgactttcgt cttattcgcc accgcatgct ggcacctgaa aaaatgggct
    62101 ggaaatacaa agacgcctgg atgcaggaac atgaggcgga aattgcacag ttaattcagc
    62161 atattcatga taaggggccg gtacgttcag ccgattttga gtatcctcgt aaaggtgcaa
    62221 gcggctggtg ggaatggaag ccgcataaac ggcatctgga aggtttattt actgccggaa
    62281 aggtgatggt gattgaacgg cgcaacttcc agcgcgttta tgatttaacc caccgtgtca
    62341 tgcctgactg ggatgatgag cgcgatctcg tttcgcaaac agaagcagaa atcatcatgc
    62401 tggataacag tgcgcgtagc cagggaatat tccgcgaaca gtggctggca gattactatc
    62461 ggctgaaacg tccggcactg gcagcgtggc gcgaagcgag ggctgaacag cggcaaatca
    62521 ttgctgtgca tgttgaaaaa ttgggcaatc tttggctgca tgctgatttg ctgccgctac
    62581 tcgagcgagc gctggccgga aagctcactg caacgcacag cgcggtactt tcgccttttg
    62641 atcctgttgt ctgggatcgc aaacgcgcag agcagctttt tgattttagc taccggctgg
    62701 agtgctatac cccagcgccg aaacgccagt atggctattt tgttctgccg ttattacatc
    62761 gtgggcaatt agttgggcga atggatgcca aaatgcatcg ccagacaggc atccttgaag
    62821 ttatctctct gtggttacag gaaggtatta aaccaacgac aacgctgcaa aaagggttac
    62881 gtcaggcgat tactgatttc gctaactggc agcaggcaac gcgggtgaca ttaggacact
    62941 gcccgcaagg tctctttacg gattgccgca ccggctggga aatagacccc gtcgcataaa
    63001 tgattatgat aagatttatg cattcataac ccgatccatc tggaggaact atggatcatc
    63061 gtctgcttga aatcattgcc tgcccggttt gcaacggaaa actttggtat aaccaggaaa
    63121 aacaagaact tatttgcaaa ctggacaacc tggctttccc gctgcgcgat ggcattccag
    63181 tgttgctgga aacggaagcc cgcgtgctga ctgctgatga gagtaaatca tgagttttgt
    63241 ggtcattatt cccgcgcgct acgcgtccac gcgtctgccc ggtaaaccat tggttgatat
    63301 taacggcaaa cccatgattg ttcatgttct tgaacgcgcg cgcgaatcag gtgccgagcg
    63361 catcatcgtg gcaaccgatc atgaggatgt cgcccgcgcc gttgaagccg ctggcggtga
    63421 agtatgtatg acgcgcgccg atcaccagtc agggacagaa cgcttggcgg aagttgtcga
    63481 aaaatgcgcg ttcagcgacg acacggtgat cgttaatgtg cagggtgatg aaccgatgat
    63541 ccctgcgaca atcattcgtc aggttgctga taacctcgct cagcgtcagg tgggtatggc
    63601 gactctggcg gtgccaatcc acaatgcgga agaagcgttt aacccgaatg cggtgaaagt
    63661 ggttctcgac gctgaagggt atgcactgta cttctcacgc gctaccattc cgtgggatcg
    63721 tgatcgtttt gcaaaaggcc ttgaaaccgt tggcgataac ttcctgcgtc atcttggtat
    63781 ttatggctac cgtgcaggct ttatccgtcg ttacgtcacc tggcagccaa gcccgttaga
    63841 acacatcgaa atgttagagc agcttcgtgt tttgtggtac ggcgaaaaaa tccatgttgc
    63901 tgtcgcgcat gaagttcctg gcacaggtgt ggatacccct gaagatctcg agcgcgttcg
    63961 cgctgaaatg cgctaatttc acttcacgac acttcagcca attttgggag gagtgtcgta
    64021 ccgttacgat tttcctcaat ttttcttttc aacaattgat ctcattcagg tgacatcttt
    64081 tatattggcg ctcattatga aagcagtagc ttttatgagg gtaatctgaa tggaacagct
    64141 gcgtgccgaa ttaagccatt tactgggcga aaaactcagt cgtattgagt gcgtcaatga
    64201 aaaagcggat acggcgttgt gggctttgta tgacagccag ggaaacccaa tgccgctaat
    64261 ggcaagaagc tttagtacgc ccggaaaagc ccgacaactg gcatggaaaa ccaccatgct
    64321 ggcaagaagt gggactgtcc gtatgccgac tatttatggt gtgatgacgc atgaagaaca
    64381 ccccggccct gatgtcctgc tactggagcg gatgcgtggt gtttcggtag aggcaccagc
    64441 ccgaacacca gaacgttggg aacagctcaa agaccaaatc gttgaagcct tactggcctg
    64501 gcaccgtcag gacagtcgcg gttgcgtcgg cgcggtcgac aatactcagg aaaatttctg
    64561 gccctcatgg taccggcaac atgttgaagt gctatggacc acgctcaatc agttcaataa
    64621 caccggtctg acgatgcagg ataagcggat cctgtttcgc actcgcgaat gtctcccggc
    64681 attatttgaa ggttttaacg acaattgtgt gctgattcac ggtaacttct gtttacgcag
    64741 catgttgaaa gattcgcgca gcgatcagtt actggcgatg gtcgggccgg gactaatgct
    64801 ttgggcaccg cgagaatacg aactgttccg actaatggat aattctctgg cggaagattt
    64861 gctctggagt tacctgcaac gcgcgccagt ggcggagtcg ttcatctggc ggcgttggtt
    64921 gtatgtgtta tgggatgaag ttgcgcaact ggttaatacc ggacgattta gtcggcgcaa
    64981 cttcgatctg gcatcaaaat cactcttgcc gtggctcgcc tgacgaacct tttagccact
    65041 gccagatgcg gccaagcgtt tcatagccaa cacgatcgct atgcatcaac cagaccggag
    65101 aagggattgc ccgttcccac gggttaagcg gcgagtcgat ggccaactga ttcgccgggg
    65161 ctggtagtgg atttaaccct tcctgctgaa aaaagatcat cgcccgcggc agatgtgatg
    65221 cggaggtcac cagcaggaaa ggggcgtcac caatcgcctg tttcactgcc gcagcttctt
    65281 cttcggtatc ttttggcaaa tccagggtga taatttgctc gcgcggtacg cccagcgatt
    65341 gcgcaactct ggcacctact tccgctgtac tcaccgtatt ggtttttgct acgcctcccg
    65401 tgaagatcag ttttgaaccc ggattttcgc gccataaacg gataccctca ttcaggcgtg
    65461 gcagactgtt attgattaaa ttagagctcg gtgcccactg cgggttccag gtataaccac
    65521 ctccgagcac cacgatatag tccacttttt gcgaattatt ccatgtcgga taggtgcttt
    65581 cgatgggacg cagcaggcgg tcggctaccg gttgcaaact caataacaag agtgccagcc
    65641 acccaatgct aataaaaatc ttcccggttt tttgaaaacg gctaaaccac agtagcgcca
    65701 ggccagcgcc aataattaac aacatcagcg gaagtggcag caacatattg ccaatcactt
    65761 ttttcagtgt aaaaagcatc ctttctggtt ccttttttaa ccatatagca agggatcgcc
    65821 agcgatatta caccagacgg gttcattctc gccgtggctg tgacaaaata gcgtctttat
    65881 cagaaaacgt cttagccaac cggtggagaa agcaatgcag gatcgcaatt ttgatgatat
    65941 tgcggaaaag ttttcccgta acatttacgg caccaccaaa gggcagcttc gacaggctat
    66001 tctgtggcag gatctcgatc gcgtgctggc ggaaatgggt ccgcaaaaac tgcgtgtgct
    66061 ggatgcgggc ggtggagaag ggcagaccgc aatcaaaatg gccgagcgcg ggcatcaggt
    66121 cattttatgc gatctttctg cgcagatgat cgaccgcgca aaacaggcgg cagaagcaaa
    66181 aggtgtgagc gacaacatgc aatttataca ttgcgccgct caggatgttg cttcgcattt
    66241 ggaaacgccc gttgatctga tattgttcca tgcggtgctc gagtgggtgg ctgatccccg
    66301 cagcgtattg cagaccctgt ggtcagtgtt gcgtccaggc ggcgtgttgt cgttaatgtt
    66361 ctacaatgcg catggtttgt tgatgcataa catggtcgcc gggaattttg attacgtgca
    66421 ggcgggaatg ccgaaaaaga aaaaacggac gctttcgcca gattatccac gcgacccgac
    66481 gcaggtttat ctgtggctgg aagaagctgg ttggcaaatt atgggtaaga caggcgttcg
    66541 cgtgtttcat gattatctgc gcgagaagca ccagcagcgc gactgctatg aagcattact
    66601 tgaattagaa acgcgttatt gccgtcagga accgtatatt accctggggc gttatattca
    66661 tgtcaccgcg cgcaaaccgc agagcaagga taaagtatga gtgaattttc ccagacagtc
    66721 cccgaactgg ttgcctgggc cagaaaaaat gacttctcca tttcgctgcc ggtagaccga
    66781 ctctcttttc tgctggcagt tgccacgctg aacggcgagc gtctggatgg tgagatgagt
    66841 gaaggcgagc tggtggatgc attccgccat gtgagtgatg cgtttgagca aaccagcgaa
    66901 accatcggcg tgcgcgccaa caacgcgatc aacgacatgg tgcgtcaacg tctgctgaac
    66961 cgctttacca gcgagcaggc agaagggaac gcaatttacc gtctgacgcc gctcggcatc
    67021 ggcattactg actattacat ccgtcagcgc gagttttcta cgctgcgcct ttctatgcag
    67081 ttgtcgattg tggcgggtga gctcaaacgc gcagcagatg ccgccgaaga gggcggtgat
    67141 gaatttcact ggcaccgtaa tgtctatgcg ccactgaaat attcggtagc agaaattttc
    67201 gacagtatcg acctgacgca acgtctgatg gacgaacagc agcagcaggt gaaggacgat
    67261 atcgcccagt tgctgaacaa agactggcgg gcggcgattt ccagctgtga attgttgctt
    67321 tcggaaactt ccggaacgct gcgtgaattg caggatacac tggaagcggc aggcgacaaa
    67381 ttgcaggcta atctgttgcg cattcaggat gcgacgatga ctcatgacga tctgcatttc
    67441 gtcgatcgtc tggtgttcga tctgcagagc aaactcgacc gtattatcag ttggggccag
    67501 caatccatcg atttgtggat tggctacgac cgccacgtac ataaatttat tcgtaccgcg
    67561 atcgatatgg ataaaaaccg cgtctttgct cagcggttac gtcagtcggt acaaacctat
    67621 tttgatgagc cgtgggcgct aagttatgcc aatgccgatc gtctgctgga tatgcgtgac
    67681 gaagagatgg cactgcgcga tgaagaagtg actggggaac ttccgccaga tttggaatac
    67741 gaagagttta acgagatccg cgaacagctg gcggcgatca tcgaagaaca acttgccgtg
    67801 tacaaaacca gacaagtgcc gctggatctt ggtctggtgg tacgcgaata tctgtcacag
    67861 tatccgcgtg cacgtcactt tgacgttgcg cgcattgtta ttgatcaggc ggtacgtctt
    67921 ggcgtagcgc aagcagattt caccggactg ccagcgaaat ggcagccgat taatgattac
    67981 ggagccaagg tacaggcgca tgtcatcgac aaatattgaa caagtgatgc cggttaagct
    68041 ggcgcaggcg ctggcgaatc cgttatttcc ggcgctggac agcgccttac gttcaggccg
    68101 ccatattggc ctcgacgaac tggataatca tgcattcctg atggattttc aggaatatct
    68161 ggaagagttt tacgcgcgtt ataacgttga gcttattcgt gcaccggaag ggttcttcta
    68221 tttacgtcca cgttccacca cgctgatccc tcgttccgtc ttgtcggaac tggatatgat
    68281 ggtcgggaaa atcctctgtt atctctatct cagcccggaa cggctggcga atgaggggat
    68341 tttcacccag caggaactgt acgacgaact gctcaccctg gccgatgaag cgaaactgct
    68401 gaaactggtg aacaaccgtt caaccggttc agacattgac cgtcagaagt tgcaggaaaa
    68461 agtacgttct tcgctcaacc gtctgcgtcg tttaggtatg gtgtggttta tgggccacga
    68521 cagcagcaag ttccgcatta ccgaatcggt gttccgcttc ggagccgatg tgcgtgctgg
    68581 cgacgatccc cgtgaagcac agcgtcgcct gattcgtgat ggcgaagcaa tgccgattga
    68641 aaatcatctg caactcaacg atgaaaccga agagaatcag ccagatagcg gagaggaaga
    68701 ataatgattg aacgcggtaa atttcgctca ctgacgctga ttaactggaa cggcttcttt
    68761 gcccgaactt ttgaccttga cgagctggtc acgacgcttt ctggcggtaa cggggcgggt
    68821 aaatccacca ccatggcggc gttcgttacg gcgctgatcc ccgacctgac cctgctgcat
    68881 ttccgtaaca ctacggaagc cggggccacc agcggttcgc gcgataaagg tctgcacggt
    68941 aagctgaaag cgggtgtctg ttattcgatg ctcgacacca ttaactcgcg ccaccagcgc
    69001 gtggtggtcg gtgtgcgtct gcaacaggtt gccggacgcg atcgtaaagt ggatatcaaa
    69061 ccatttgcca ttcagggact gccgatgtcg gtgcagccga cacagctggt gaccgaaact
    69121 ctgaacgaac gccaggcgcg cgtgctgccg cttaacgagc tgaaagacaa gctcgaggcg
    69181 atggaaggcg tgcagtttaa acagttcaac tccattaccg attaccactc gctgatgttc
    69241 gatctgggca tcattgcgcg tcgtctgcgc tccgcatctg accgtagcaa attctatcgt
    69301 ctgatcgaag cttcgctgta tggcgggatc tccagtgcca ttacccgttc tctgcgcgac
    69361 tacctgttgc cggaaaacag cggcgtgcgt aaagcgttcc aggacatgga agcggcgctg
    69421 cgtgaaaacc gcatgacgct ggaagcgatt cgtgtcaccc agtcggaccg tgacctgttt
    69481 aagcatctga tcagcgaagc taccaactac gtggcggcgg actacatgcg tcacgccaac
    69541 gagcgccgtg tccatctcga taaggccctg gagtttcgtc gcgaactaca tacttcgcgt
    69601 cagcaactgg cggcggagca gtacaaacac gtcgatatgg cgcgtgagct ggcagagcac
    69661 aacggtgccg aaggcgatct ggaagcggat tatcaggcgg ccagcgatca cctgaacctg
    69721 gtgcaaaccg cactgcgtca gcaggagaag atcgaacgct acgaagcgga tctcgatgag
    69781 ctgcagatcc gtctggaaga gcaaaacgaa gtggtggcag aagccatcga acgccaggaa
    69841 gagaacgagg ctcgcgcgga agctgccgaa ctggaagtgg acgagctgaa aagccagctt
    69901 gctgactacc agcaggcgct ggacgtccag caaacgcgcg cgatccagta caaccaggcg
    69961 attgctgcgc ttaatcgtgc caaagaactg tgccatctgc cggacttaac cgccgacagc
    70021 gccgccgaat ggctggaaac cttccaggcg aaagagctgg aagcgactga aaaaatgctc
    70081 tctcttgagc agaaaatgag catggcgcaa accgcgcaca gccagtttga gcaggcttat
    70141 cagctggtgg tggcaatcaa cggcccgctg gcgcgtaacg aggcgtggga tgtcgctcgc
    70201 gaactattgc gcgaaggggt cgatcagcgt cacctggcag agcaggttca gccgttgcgg
    70261 atgcgcttaa gcgaactgga acagcgtctg cgcgaacagc aagaagctga gcgtctgctg
    70321 gcagatttct gcaaacgtca gggcaagaat tttgacattg acgagctgga agccctgcat
    70381 caggagctgg aagcacgcat tgcctctctt tccgatagcg tgtctaacgc ccgtgaagag
    70441 cggatggcac tgcgccagga gcaggaacag ctgcagtctc gcattcagag tttgatgcag
    70501 cgtgcaccgg tttggctggc agcgcaaaac agtctcaacc agttgagcga acagtgcggc
    70561 gaagagttca cctccagcca ggacgtcact gaatatctgc aacagttgct ggagcgtgag
    70621 cgcgaggcca ttgttgaacg cgatgaagta ggcgcgcgca aaaacgccgt cgatgaagag
    70681 atcgaacgtt taagccagcc tggcggctct gaagatcagc gtctgaacgc gctagcggag
    70741 cgttttggtg gtgtgctgct gtcagaaatt tatgacgacg ttagcctgga agatgcgccg
    70801 tacttctcag cgctgtatgg cccgtcacgc cacgccatcg tggtgccaga tctgtcacag
    70861 gtaaccgaac acctggaagg tttgaccgat tgcccggaag atctctattt gatcgaaggg
    70921 gatccgcagt cattcgatga cagcgtgttc agcgttgatg agctggaaaa agcggtagtg
    70981 gtgaaaatcg ccgatcgtca gtggcgttat tcacgtttcc cggaagtgcc gctgtttggt
    71041 cgtgctgcgc gtgaaagccg tattgaaagc ctccatgccg agcgtgaagt gctttccgaa
    71101 cgcttcgcca cgctctcctt tgatgtacag aaaacccagc gtctgcatca ggcgttcagc
    71161 cgctttatcg gcagtcatct ggcggtggcg tttgagtctg acccggaagc agaaatccgt
    71221 caactgaaca gccgtcgcgt cgaactggag cgggcgttga gtaatcatga aaatgataac
    71281 cagcagcagc gtattcagtt tgagcaggcg aaagaaggcg ttacggcgct gaaccgcatt
    71341 ctgccgcgtc tcaacctgtt ggctgatgac agcctggcgg atcgcgtcga tgaaattcgc
    71401 gaacgtctgg atgaagccca ggaagccgcg cgttttgttc agcagtttgg caatcaactg
    71461 gcgaaactgg aaccgatcgt ttcggtattg cagagcgacc cggaacagtt cgaacagtta
    71521 aaagaagatt acgcgtactc tcagcagatg cagcgcgatg cccgtcagca ggcgtttgcc
    71581 ctgacggaag tggtgcagcg tcgtgcgcac tttagctact ctgactcggc agaaatgctt
    71641 agcggtaaca gcgatctcaa cgaaaaactg cgtgaacgtc tggaacaggc ggaagcggag
    71701 cgtacccgcg ctcgcgaagc gttgcgcggt cacgcagcgc agttgagtca gtacaaccag
    71761 gtgctggctt cgctgaaaag ttcttacgac accaaaaaag agctactcaa cgatctgcaa
    71821 cgtgaattgc aggatatcgg cgtgcgtgct gatagcgggg cagaagagcg ggcacgtatt
    71881 cgccgtgacg agctgcatgc acaactgagc aataaccgtt cgcgccgcaa tcaactggaa
    71941 aaagcgctca ccttctgcga agcggagatg gataacctga cccgcaaact gcgcaagctg
    72001 gagcgtgatt actttgagat gcgcgagcag gtagtgaccg cgaaagcggg ctggtgtgca
    72061 gtcatgcgca tggtgaaaga taacggcgtt gagcgccgct tacaccgtcg tgagctggct
    72121 tatctctccg ctgatgattt gcgttccatg tcggataagg cgttaggtgc gctgcgtctg
    72181 gcggtggcgg ataacgaaca tctgcgcgac gtgctgcgca tgtcggaaga tcccaaacgt
    72241 ccggagcgta aaattcagtt cttcgtggcg gtttatcagc atctgcgtga acgtattcgt
    72301 caggatatta ttcgtaccga tgatccggtg gaagctatcg aacagatgga gatcgaactt
    72361 agccgtctga ccgaagagtt aacctcccgt gaacagaaac tggcgatcag ttcccgcagc
    72421 gtggcgaaca tcattcgcaa aaccattcag cgcgagcaga accgtatccg tatgctcaac
    72481 caggggttgc agaacgtatc gtttggtcag gtgaacagcg tgcgcctcaa cgtgaacgtg
    72541 cgtgaaacgc acgccatgct gctggatgtg ctctctgaac agcatgagca gcatcaggat
    72601 ctgtttaaca gcaaccgtct gaccttctcg gaagcgctgg cgaaattgta tcagcgtctt
    72661 aatccgcaga ttgatatggg gcagcgcacg ccgcagacca ttggcgaaga actgctggat
    72721 taccgcaact atctggaaat ggaagttgag gttaaccgtg gttccgatgg ctggttgcgc
    72781 gcagagtctg gtgcattgtc gaccggtgag gcgattggta ccggtatgtc gattctggtg
    72841 atggtggtac aaagctggga agatgaatct cgccgcctgc gcggtaaaga tatctctcct
    72901 tgccgcctgc tgttcctcga tgaagcagcg cgactggatg ctcgttctat cgccacgctg
    72961 tttgaattgt gtgagcgttt gcaaatgcag ctcatcatcg cagcgccgga aaatatcagc
    73021 ccggagaaag gcaccaccta taaactggtg cgtaaagtct tccagaatac cgaacatgtt
    73081 catgtcgtcg gcctgcgagg atttgcgccg caactccctg aaacgcttcc aggaagtgac
    73141 gaagcgcctt ctcaggcgag ttaaaattaa ggcggcagca atgccgcctt ttctttttcc
    73201 gaaaactccg tttctgcact aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc
    73261 tttacattag gttatgtaaa aacgtatcgg cgtttatata ctgaagataa gcctgatgag
    73321 taacaggctt gctcgtcata ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat
    73381 aacagcctgg ctattcagag tatgataaaa acagggggca agggatgttg cttaatatga
    73441 tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc cgtaacattc gctccactgt
    73501 tcaatgcgca ggccgatgag cctgaagtaa tccctggcga cagcccggtg gctgtcagtg
    73561 aacagggcga ggcactgccg caggcgcaag ccacggcaat aatggcgggg atcctgccat
    73621 tgcctgaagg tgcggcagaa aaagcccgca cgcaaatcga atctcaatta cccgcaggtt
    73681 acaagccggt ttatcttaac cagcttcaac tgttgtatgc cgcacgcgat atgcaaccca
    73741 tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca gctggcagag gtggcgattg
    73801 ccggtttcca gccgcagttt aataaatggg tagagttact gaccgatcct ggtgttaacg
    73861 ggatggcacg cgacgtggtg ctctctgatg cgatgatggg ctatctccat ttcattgcaa
    73921 atattccggt caaaggcact cgctggctat atagcagtaa accttatgcg cttgcaacgc
    73981 cgccgctttc ggtgattaac caatggcagc aggcgctgga taaaggtcaa ttgcctacgt
    74041 ttgttgcagg actggcaccg cagcatccgc aatatgcggt gatgcatgaa tcgttactgg
    74101 ccttactcag tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag
    74161 ggcagtggag taacgacgta ccggcgttgc gtgaaatatt gcaacgcaca ggcatgttgg
    74221 acggggggcc gaaaattact ctacctggcg atgacacgcc aactgacgcg gtagtcagcc
    74281 catccgctgt tactgttgaa acagcagaaa ctaagccgat ggataagcaa acgacgtctc
    74341 gtagtaaacc tgcgcctgcc gttcgcgccg cctacgataa tgaactggtg gaagccgtta
    74401 aacgttttca ggcatggcaa ggattggggg cagatggtgc tattggcccg gcaacgcgtg
    74461 actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt ggctctcaac atccagcgat
    74521 tgcgcttgct gccaacagag ctttctaccg ggatcatggt taacattccg gcctattcgc
    74581 tggtctacta tcagaacggc aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg
    74641 atcgcaaaac gccgatgatg agcagtgccc ttaacaatgt agtggtaaac ccgccgtgga
    74701 acgtacctcc aactctggca cgcaaagata ttctgccgaa agtgcgcaac gatccgggat
    74761 atctcgaaag ccatggctat acggtgatgc gcggctggaa cagcagagaa gcgattgacc
    74821 catggcaggt tgactggtct acaatcacgg cctcgaattt accgtttcgc ttccaacagg
    74881 ctccaggccc acggaactcg ctggggcgct ataaattcaa tatgccgagt tcagaggcca
    74941 tttatttgca tgacacgccg aaccacaatc tgttcaagcg tgatacacgc gcattgagct
    75001 caggctgtgt acgagtgaat aaagcttccg atctggcgaa tatgctgttg caggatgcag
    75061 gctggaatga caaacgtatt tctgatgcgc tgaagcaggg tgatacacgt tacgtcaata
    75121 ttcggcagtc gattccggtg aatctctact acctgacggc ctttgttggt gcagatggtc
    75181 gtacccagta tcgtacagat atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa
    75241 tcgtatcgaa agcggaacaa ttaatcaggt aaatgaagta gttcggggaa atcagttgtc
    75301 gtaataatta gcatgattgg gggcaattct ctgcagcccc cgtcactgct ggggttgagt
    75361 catcttgacg tctgctttac gggcggttaa ggtgcctctt gtgcgccaga agtgcatata
    75421 aacgataaca ttgacctgta gacttgatta tcatggacaa attcgacgct aatcgccgca
    75481 aattgctggc gcttggtggc gttgcactcg gtgccgccat cctgccgacc cctgcgtttg
    75541 caacactctc taccccacgc ccgcgcattt tgacactcaa taatcttcat accggagagt
    75601 caatcaaagc ggagtttttc gatggcagag gctatattca ggaagaattg gcaaaactta
    75661 accatttttt ccgcgattac cgcgcgaaca aaataaagtc catcgaccca ggattattcg
    75721 accagttgta tcgcctgcaa gggttattag gcacccgcaa accggtgcaa ctcatttccg
    75781 gttatcgttc cattgatacc aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga
    75841 aaagctatca cactaaaggc caggcgatgg atttccatat tgaaggtatc gcgttaagca
    75901 atattcgcaa agccgcgtta tctatgcgcg caggtggtgt aggatattac ccacgtagta
    75961 actttgtgca tattgatacc gggccagcac ggcactggta gtaattgctt aacgaaacag
    76021 gggcagtatg aactatcgta ttattccggt caccgcattc tcccagaact gttcattgat
    76081 ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat
    76141 caaacaggaa gttgacgcca gcggcctgac actgatgcag atcctgctga cgcatggtca
    76201 tctggaccac gttggcgcag cggcggaact ggcgcaacat tacggcgtgc cggtttttgg
    76261 cccggaaaaa gaagatgagt tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg
    76321 tctggaagag tgccagccgc tgacgccaga tcgttggctg aacgaaggcg ataccatcag
    76381 catagggaat gtgactttac aggtgttaca ttgccctggg catacgccgg gtcatgtcgt
    76441 gttttttgat gatcgggcaa agctgctgat ttctggcgat gttattttca aaggcggagt
    76501 agggcgcagt gacttcccgc gtggcgatca taatcaactg atttcttcaa tcaaagataa
    76561 attgctgcca ctgggtgatg acgtgacatt tattccgggt cacggaccat tatccacact
    76621 tggttatgaa cgcctgcata atcccttcct gcaagacgaa atgcccgtct ggtaaggcac
    76681 acataaaaaa gcccgctttt cagcgggctt cattgttttt aacgcttaca gcactgccac
    76741 aatcgcttcg cacagcggag ccatgttatc tggtgtcatc ccggccacgt ttacgcgacc
    76801 agaagcaaca gcatacacgc caaactcttc gcgcagacgc agcacttgtt cttttgtcag
    76861 gccactgaag gagaacatgc cgttctgttt gatgataaag ctgaagtcgc ggtttgcgcc
    76921 tttttcctgc agcgtattga cgaacaactg acgcatacgc tgaatacgct ggcgcatatc
    76981 agtcagctct tgttcccaaa tcgcacgtaa cgcatcgttg ctcaggatgg tggcaacaac
    77041 agaagcgccg tgtgctggtg ggttagagta gttagcgcga atcgccgctt tcatttggct
    77101 gaatgcgcga tcaacggttt cactgtcggc agcaaccaga gtacaagcgc caacacgctc
    77161 gttgtacagg ccaaagtttt tagagtagga actggcaaca atcagctctt tatgcatagc
    77221 cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca cgggcaaaac cctggtaagc
    77281 gaagtcaaac agcggtaacc agcctttctc aacggagagt tgtgccagtg tctgccattg
    77341 ttccagcgta gggtcgatac cggttgggtt gtggcagcag ccatggaaca gcactacgtc
    77401 gccagcctga gcttcgttca ggctgttaat cagtgcatcg aagtcaaggg tgtggttttc
    77461 cgcatcataa taagcgtatt cacgaacttc cagatctgca gagttaaaga cgctcttatg
    77521 gttcggccag cttgggttgc tcacccacac tcgcttaacg ctggtatttt ttgccaggaa
    77581 atcggcagct atgcgtagtg cgccagtgcc acccggagtc tgtgccgtgc gagcacgttt
    77641 gtcattgatc agggcgctac ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc
    77701 agggatgccg tcaatgccga ggtaattttt ggtggtttca ttttcgagca gatactgttc
    77761 agcctttttc acgctggtca gtaccggggt tttgcccgtc tcatctttat agacaccaat
    77821 cccgaggtta attttgccgg gacgttcatc ggcacgaaac agatcggcca ggcccagaat
    77881 cgggtcggca ggagcggcgg taatgttctc aaacatgacg aggttccatt atggttacag
    77941 aagggaagtc cgctatcagg gtaacgggag atttacaaaa ttccaactat tactgatgaa
    78001 aacgcaggct gtttttgcaa gacgtgagat tgctctggaa ggcataaaaa aaacaggacc
    78061 aaagtcctgt tttttcggca tttaacaaag aggtgtgcta ttagaactgg taaacgatac
    78121 ccacagcaac ggtgtcgtct gaacctacgc ccagtttgtt gtcagaatcg atctggttga
    78181 tgatgtagtc aacataggtg gacatgtttt tgttgaagta gtaggttgcg cccacttcaa
    78241 agtagttcac cagatcaaca tcaccgatac cttctacgtc tttcgcttta gatttggtgt
    78301 aagcgatgga cggacgcaga ccgaaatcga actggtattg cgcaactaac agaacgtctt
    78361 gcgttttgtt ggcgaagccg ctgatgtttg taaatttatt agtgatcggc gtagcgttac
    78421 gcgtttcacc gtagttcgct gccaggtaga tgttgttcgc atcatacttc agaccagtag
    78481 cccactgttc agctttttta ccgttgccaa gaagttgagc ttcttgcagg ttggtacggt
    78541 cagctgcacc ataagcacca acgataccaa agccttcgta ttcgtagctg atagaaccgc
    78601 caacaccgtc gccgttagag cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa
    78661 cagcgaagtt caggccatca accagaccaa agaagttgga gttacgatag gtagcaacgc
    78721 cgccaacacg accaacgaag aagtcatcgc tgtatgcagt atcaccacca aattctggca
    78781 gcatatcggt gtaacccagt gcatcataaa ccacaccgta gttacggccg taatcgaaag
    78841 aaccaacgtc agcgtattta agacccgcga atgccagacg cgttttgtta ccagtttgag
    78901 cgtcagcgcc ttcagagttg ttaccctgga agttatattc ccactgacca taaccggtca
    78961 gatcggaatt gatttgagtt tcccctttaa aaccaagacg ggcataggtc atgtcgccat
    79021 tgccaccgta actgttttca ccgttaccct tggagaaata atgcagaccg acagctttac
    79081 cgtacagatc tactttgttg ccatctttgt tatagatttc tgcagcgttt gcagtacctg
    79141 ctactaacag agcagggacg atcactgcca gaatattgcg cttcatcatt atttattacc
    79201 ctcatggttt tttttatgac acctgccact gccgtcaata atttctgtca ataaaatttt
    79261 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
    79321 ctaacgtttc gctacc
//
LOCUS       DAZGXS010000007        75439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGXS010000007 DAZGXS010000000
VERSION     DAZGXS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..411
                     /locus_tag="ACHKG5_000553"
     CDS             <1..411
                     /locus_tag="ACHKG5_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0866725.1"
                     /translation="DVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKN
                     SLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSG
                     HGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(454..1314)
                     /gene="rsmI"
                     /locus_tag="ACHKG5_000554"
     CDS             complement(454..1314)
                     /gene="rsmI"
                     /locus_tag="ACHKG5_000554"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0866726.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1378..3414
                     /gene="lpoA"
                     /locus_tag="ACHKG5_000555"
     CDS             1378..3414
                     /gene="lpoA"
                     /locus_tag="ACHKG5_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0866727.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3372..3767
                     /gene="yraN"
                     /locus_tag="ACHKG5_000556"
     CDS             3372..3767
                     /gene="yraN"
                     /locus_tag="ACHKG5_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0866728.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3787..4377
                     /gene="diaA"
                     /locus_tag="ACHKG5_000557"
     CDS             3787..4377
                     /gene="diaA"
                     /locus_tag="ACHKG5_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0866729.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4387..4962
                     /gene="dolP"
                     /locus_tag="ACHKG5_000558"
     CDS             4387..4962
                     /gene="dolP"
                     /locus_tag="ACHKG5_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0866730.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5076..6116)
                     /gene="yraQ"
                     /locus_tag="ACHKG5_000559"
     CDS             complement(5076..6116)
                     /gene="yraQ"
                     /locus_tag="ACHKG5_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0866731.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6189..6824)
                     /gene="yraR"
                     /locus_tag="ACHKG5_000560"
     CDS             complement(6189..6824)
                     /gene="yraR"
                     /locus_tag="ACHKG5_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0866732.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6952..7470
                     /gene="yhbO"
                     /locus_tag="ACHKG5_000561"
     CDS             6952..7470
                     /gene="yhbO"
                     /locus_tag="ACHKG5_000561"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0866733.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7450..7893)
                     /gene="yhbP"
                     /locus_tag="ACHKG5_000562"
     CDS             complement(7450..7893)
                     /gene="yhbP"
                     /locus_tag="ACHKG5_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0866734.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7944..8246
                     /gene="yhbQ"
                     /locus_tag="ACHKG5_000563"
     CDS             7944..8246
                     /gene="yhbQ"
                     /locus_tag="ACHKG5_000563"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0866735.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8233..8736)
                     /gene="yhbS"
                     /locus_tag="ACHKG5_000564"
     CDS             complement(8233..8736)
                     /gene="yhbS"
                     /locus_tag="ACHKG5_000564"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0866736.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8730..9254)
                     /gene="ubiT"
                     /locus_tag="ACHKG5_000565"
     CDS             complement(8730..9254)
                     /gene="ubiT"
                     /locus_tag="ACHKG5_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0866737.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9463..10458
                     /gene="ubiU"
                     /locus_tag="ACHKG5_000566"
     CDS             9463..10458
                     /gene="ubiU"
                     /locus_tag="ACHKG5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0866738.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10467..11345
                     /gene="ubiV"
                     /locus_tag="ACHKG5_000567"
     CDS             10467..11345
                     /gene="ubiV"
                     /locus_tag="ACHKG5_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0866739.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11426..12433
                     /gene="yhbW"
                     /locus_tag="ACHKG5_000568"
     CDS             11426..12433
                     /gene="yhbW"
                     /locus_tag="ACHKG5_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP0866740.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12550..13794)
                     /gene="mtr"
                     /locus_tag="ACHKG5_000569"
     CDS             complement(12550..13794)
                     /gene="mtr"
                     /locus_tag="ACHKG5_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0866741.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13948..15837)
                     /gene="deaD"
                     /locus_tag="ACHKG5_000570"
     CDS             complement(13948..15837)
                     /gene="deaD"
                     /locus_tag="ACHKG5_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0866742.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYIHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15830..15910)
                     /gene="yrbN"
                     /locus_tag="ACHKG5_000571"
     CDS             complement(15830..15910)
                     /gene="yrbN"
                     /locus_tag="ACHKG5_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0866743.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16017..16901)
                     /gene="nlpI"
                     /locus_tag="ACHKG5_000572"
     CDS             complement(16017..16901)
                     /gene="nlpI"
                     /locus_tag="ACHKG5_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0866744.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17010..19145)
                     /gene="pnp"
                     /locus_tag="ACHKG5_000573"
     CDS             complement(17010..19145)
                     /gene="pnp"
                     /locus_tag="ACHKG5_000573"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0866745.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="ACHKG5_000574"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="ACHKG5_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0866746.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="ACHKG5_000575"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="ACHKG5_000575"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0866747.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="ACHKG5_000576"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="ACHKG5_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0866748.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21320..23992)
                     /gene="infB"
                     /locus_tag="ACHKG5_000577"
     CDS             complement(21320..23992)
                     /gene="infB"
                     /locus_tag="ACHKG5_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0866749.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24017..25504)
                     /gene="nusA"
                     /locus_tag="ACHKG5_000578"
     CDS             complement(24017..25504)
                     /gene="nusA"
                     /locus_tag="ACHKG5_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0866750.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25532..25984)
                     /gene="rimP"
                     /locus_tag="ACHKG5_000579"
     CDS             complement(25532..25984)
                     /gene="rimP"
                     /locus_tag="ACHKG5_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0866751.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26191..26267)
                     /locus_tag="ACHKG5_000580"
     tRNA            complement(26191..26267)
                     /locus_tag="ACHKG5_000580"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26231..26233),aa:Met,seq:cat)
     gene            26615..27958
                     /gene="argG"
                     /locus_tag="ACHKG5_000581"
     CDS             26615..27958
                     /gene="argG"
                     /locus_tag="ACHKG5_000581"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0866752.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27966..29591)
                     /gene="yhbX"
                     /locus_tag="ACHKG5_000582"
     CDS             complement(27966..29591)
                     /gene="yhbX"
                     /locus_tag="ACHKG5_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0866753.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(30050..30136)
                     /locus_tag="ACHKG5_000583"
     tRNA            complement(30050..30136)
                     /locus_tag="ACHKG5_000583"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30100..30102),aa:Leu,seq:gag)
     gene            complement(30151..30483)
                     /gene="secG"
                     /locus_tag="ACHKG5_000584"
     CDS             complement(30151..30483)
                     /gene="secG"
                     /locus_tag="ACHKG5_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0866754.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30709..32046)
                     /gene="glmM"
                     /locus_tag="ACHKG5_000585"
     CDS             complement(30709..32046)
                     /gene="glmM"
                     /locus_tag="ACHKG5_000585"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0866755.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32039..32887)
                     /gene="folP"
                     /locus_tag="ACHKG5_000586"
     CDS             complement(32039..32887)
                     /gene="folP"
                     /locus_tag="ACHKG5_000586"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0866756.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32977..34911)
                     /gene="ftsH"
                     /locus_tag="ACHKG5_000587"
     CDS             complement(32977..34911)
                     /gene="ftsH"
                     /locus_tag="ACHKG5_000587"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0866757.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35011..35640)
                     /gene="rlmE"
                     /locus_tag="ACHKG5_000588"
     CDS             complement(35011..35640)
                     /gene="rlmE"
                     /locus_tag="ACHKG5_000588"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0866758.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35766..36059
                     /gene="yhbY"
                     /locus_tag="ACHKG5_000589"
     CDS             35766..36059
                     /gene="yhbY"
                     /locus_tag="ACHKG5_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0866759.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36215..36691)
                     /gene="greA"
                     /locus_tag="ACHKG5_000590"
     CDS             complement(36215..36691)
                     /gene="greA"
                     /locus_tag="ACHKG5_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0866760.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36939..38372
                     /gene="dacB"
                     /locus_tag="ACHKG5_000591"
     CDS             36939..38372
                     /gene="dacB"
                     /locus_tag="ACHKG5_000591"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0866761.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38412..39584)
                     /gene="cgtA"
                     /locus_tag="ACHKG5_000592"
     CDS             complement(38412..39584)
                     /gene="cgtA"
                     /locus_tag="ACHKG5_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0866762.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39600..40565)
                     /gene="yhbE"
                     /locus_tag="ACHKG5_000593"
     CDS             complement(39600..40565)
                     /gene="yhbE"
                     /locus_tag="ACHKG5_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0866763.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40692..40949)
                     /gene="rpmA"
                     /locus_tag="ACHKG5_000594"
     CDS             complement(40692..40949)
                     /gene="rpmA"
                     /locus_tag="ACHKG5_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0866764.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40970..41281)
                     /gene="rplU"
                     /locus_tag="ACHKG5_000595"
     CDS             complement(40970..41281)
                     /gene="rplU"
                     /locus_tag="ACHKG5_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0866765.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41540..42511
                     /gene="ispB"
                     /locus_tag="ACHKG5_000596"
     CDS             41540..42511
                     /gene="ispB"
                     /locus_tag="ACHKG5_000596"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0866766.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42740..43018
                     /gene="sfsB"
                     /locus_tag="ACHKG5_000597"
     CDS             42740..43018
                     /gene="sfsB"
                     /locus_tag="ACHKG5_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0866767.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43066..44325)
                     /gene="murA"
                     /locus_tag="ACHKG5_000598"
     CDS             complement(43066..44325)
                     /gene="murA"
                     /locus_tag="ACHKG5_000598"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0866768.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44380..44634)
                     /gene="ibaG"
                     /locus_tag="ACHKG5_000599"
     CDS             complement(44380..44634)
                     /gene="ibaG"
                     /locus_tag="ACHKG5_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0866769.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44794..45087)
                     /gene="mlaB"
                     /locus_tag="ACHKG5_000600"
     CDS             complement(44794..45087)
                     /gene="mlaB"
                     /locus_tag="ACHKG5_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0866770.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45087..45722)
                     /gene="mlaC"
                     /locus_tag="ACHKG5_000601"
     CDS             complement(45087..45722)
                     /gene="mlaC"
                     /locus_tag="ACHKG5_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0866771.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45741..46292)
                     /gene="mlaD"
                     /locus_tag="ACHKG5_000602"
     CDS             complement(45741..46292)
                     /gene="mlaD"
                     /locus_tag="ACHKG5_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0866772.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46297..47079)
                     /gene="mlaE"
                     /locus_tag="ACHKG5_000603"
     CDS             complement(46297..47079)
                     /gene="mlaE"
                     /locus_tag="ACHKG5_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0866773.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47087..47896)
                     /gene="mlaF"
                     /locus_tag="ACHKG5_000604"
     CDS             complement(47087..47896)
                     /gene="mlaF"
                     /locus_tag="ACHKG5_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0866774.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48106..49083
                     /gene="yrbG"
                     /locus_tag="ACHKG5_000605"
     CDS             48106..49083
                     /gene="yrbG"
                     /locus_tag="ACHKG5_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0866775.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49097..50083
                     /gene="kdsD"
                     /locus_tag="ACHKG5_000606"
     CDS             49097..50083
                     /gene="kdsD"
                     /locus_tag="ACHKG5_000606"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0866776.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50104..50670
                     /gene="kdsC"
                     /locus_tag="ACHKG5_000607"
     CDS             50104..50670
                     /gene="kdsC"
                     /locus_tag="ACHKG5_000607"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0866777.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50667..51242
                     /gene="lptC"
                     /locus_tag="ACHKG5_000608"
     CDS             50667..51242
                     /gene="lptC"
                     /locus_tag="ACHKG5_000608"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0866778.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51211..51768
                     /gene="lptA"
                     /locus_tag="ACHKG5_000609"
     CDS             51211..51768
                     /gene="lptA"
                     /locus_tag="ACHKG5_000609"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0866779.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51775..52500
                     /gene="lptB"
                     /locus_tag="ACHKG5_000610"
     CDS             51775..52500
                     /gene="lptB"
                     /locus_tag="ACHKG5_000610"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0866780.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52548..53981
                     /gene="rpoN"
                     /locus_tag="ACHKG5_000611"
     CDS             52548..53981
                     /gene="rpoN"
                     /locus_tag="ACHKG5_000611"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0866781.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54004..54291
                     /gene="hpf"
                     /locus_tag="ACHKG5_000612"
     CDS             54004..54291
                     /gene="hpf"
                     /locus_tag="ACHKG5_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0866782.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54409..54900
                     /gene="ptsN"
                     /locus_tag="ACHKG5_000613"
     CDS             54409..54900
                     /gene="ptsN"
                     /locus_tag="ACHKG5_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0866783.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            54946..55800
                     /gene="rapZ"
                     /locus_tag="ACHKG5_000614"
     CDS             54946..55800
                     /gene="rapZ"
                     /locus_tag="ACHKG5_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0866784.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55797..56069
                     /gene="npr"
                     /locus_tag="ACHKG5_000615"
     CDS             55797..56069
                     /gene="npr"
                     /locus_tag="ACHKG5_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0866785.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56283..56915
                     /gene="yrbL"
                     /locus_tag="ACHKG5_000616"
     CDS             56283..56915
                     /gene="yrbL"
                     /locus_tag="ACHKG5_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0866786.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(56912..57640)
                     /gene="mtgA"
                     /locus_tag="ACHKG5_000617"
     CDS             complement(56912..57640)
                     /gene="mtgA"
                     /locus_tag="ACHKG5_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0866787.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57637..58290)
                     /gene="elbB"
                     /locus_tag="ACHKG5_000618"
     CDS             complement(57637..58290)
                     /gene="elbB"
                     /locus_tag="ACHKG5_000618"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0866788.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58520..60856)
                     /gene="arcB"
                     /locus_tag="ACHKG5_000619"
     CDS             complement(58520..60856)
                     /gene="arcB"
                     /locus_tag="ACHKG5_000619"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0866789.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60952..61881)
                     /gene="yhcC"
                     /locus_tag="ACHKG5_000620"
     CDS             complement(60952..61881)
                     /gene="yhcC"
                     /locus_tag="ACHKG5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0866790.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62555..67015
                     /gene="gltB"
                     /locus_tag="ACHKG5_000621"
     CDS             62555..67015
                     /gene="gltB"
                     /locus_tag="ACHKG5_000621"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0866791.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVHSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67028..68446
                     /gene="gltD"
                     /locus_tag="ACHKG5_000622"
     CDS             67028..68446
                     /gene="gltD"
                     /locus_tag="ACHKG5_000622"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0866792.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68630..69757
                     /gene="yhcG"
                     /locus_tag="ACHKG5_000623"
     CDS             68630..69757
                     /gene="yhcG"
                     /locus_tag="ACHKG5_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP0866793.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADTRSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(69817..70281)
                     /gene="nanQ"
                     /locus_tag="ACHKG5_000624"
     CDS             complement(69817..70281)
                     /gene="nanQ"
                     /locus_tag="ACHKG5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0866794.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(70278..71153)
                     /gene="nanK"
                     /locus_tag="ACHKG5_000625"
     CDS             complement(70278..71153)
                     /gene="nanK"
                     /locus_tag="ACHKG5_000625"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0866795.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(71150..71839)
                     /gene="nanE"
                     /locus_tag="ACHKG5_000626"
     CDS             complement(71150..71839)
                     /gene="nanE"
                     /locus_tag="ACHKG5_000626"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0866796.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71887..73377)
                     /gene="nanT"
                     /locus_tag="ACHKG5_000627"
     CDS             complement(71887..73377)
                     /gene="nanT"
                     /locus_tag="ACHKG5_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0866797.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73486..74379)
                     /gene="nanA"
                     /locus_tag="ACHKG5_000628"
     CDS             complement(73486..74379)
                     /gene="nanA"
                     /locus_tag="ACHKG5_000628"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0866798.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74501..75292)
                     /gene="nanR"
                     /locus_tag="ACHKG5_000629"
     CDS             complement(74501..75292)
                     /gene="nanR"
                     /locus_tag="ACHKG5_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0866799.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        18413 a        20549 c        18813 g        17664 t
ORIGIN      
        1 gatgttatta aggttaattt tggattcatc aatggtcaga agtttaccac ccgcaatgcc
       61 atgccagacg gttttactcc agtagacttt gatatcactt atgactgtgg tgatacttca
      121 aagattaaaa actcgttgca aatgcgcatc gacggtacaa ctggggtagt agaccagtac
      181 aacctggtcg ccagacgaag aagttcagac aatgcgcccg atgtcggtat tcgtattgaa
      241 aatctcggcg gcggagttgc aaatattcct tttcagaacg gtatccttcc cgttgatcct
      301 tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta atctggtcgg tggtgagctg
      361 gaaacaggaa aatttcaggg cacagccacc attaccgtca tcgtgcggta aaagaagacg
      421 ttgcgttttg attaacgcaa cgcttcggca ctcttacccc tgctgctcca gcgcatactt
      481 atacagcgca tttttcttca cgccgtgaat ttctgcggcc agcgccgccg cttttttcag
      541 cggcagttct gcctgtagca gcgccagcgt gcgcagggca tcggcgggta agtcttcttc
      601 ctgtgcttta tgaccttcga caatcagcac catttcgcct ttgcgacggt tttcatcttc
      661 ctttacccac gccagcagct cgccaacggg cgcgccgtga atggtttccc aggttttggt
      721 cagctcacgc gccagaacca cgtagcggga ttcgcctaat accgcaacga tatcttccag
      781 gctatctaac agacggtggg tagattcata aaaaatcagc gtgcgcggct ccgcttcaat
      841 ggcttttagc gcatcacggc ggccttttga tttggcaggt aaaaagcctt cgtaacagaa
      901 acggtcagag ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg gtccgggtag
      961 cggcaccaca cgaatccccg cttcacggca ggtacgcacc agatggtagc caggatcgtt
     1021 aattagcggc gttccggcat cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc
     1081 cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc agcgcaaaca accgggcatt
     1141 aatcccaaaa tgttgcagca ataaaccggt gtgacgagta tcctcggcgg caatcagatc
     1201 aacggcctgt aatacctcta acgcacgctg ggtgatatcc gccagattgc cgattggcgt
     1261 cggtacaatg taaagctggc cctgagaatt atccgccgat tggtgttgtt tcattgtgtc
     1321 gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa atatcactgg atacattatg
     1381 gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc
     1441 ctgattttcg tcggttgtgg cacccatact cccgatcagt ccactgctta tatgcagggc
     1501 acggcgcagg ctgattctgc cttttatctt cagcagatgc agcaaagctc tgatgatacc
     1561 aggatcaact ggcaattact cgccattcgt gcactggtga aagaaggtaa aaccgggcag
     1621 gcggttgagt tgtttaacca actaccgcaa gaactgaacg attctcagcg tcgcgagaaa
     1681 acactgctgg cggtagagat taaactggcg cagaaagatt ttgctggcgc gcaaaacttg
     1741 ctggcgaaaa ttacacccgc cgatttagaa caaaaccagc aagcccgtta ctggcaggca
     1801 aaaatcgatg ccagccaggg gcgtccttcc attgatttac tgcgcgcgtt aattgctcag
     1861 gaaccgctgc ttggcgcgaa agaaaaacag cagaatattg atgccacctg gcaggcgctc
     1921 tcctccatga ctcaggaaca ggcgaatacg ctggtgatca acgccgacga aaatattctg
     1981 caaggctggc tggatctgca gcgcgtctgg tttgataacc gtaacgatcc cgacatgatg
     2041 aaagccggga tcgccgactg gcagaaacgt tatccgaaca atccgggcgc gaaaatgctg
     2101 ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct cgaccaacaa aatcgccctg
     2161 ctgttgccac tgaatggcca ggcagcggta tttggtcgca ctattcagca aggctttgaa
     2221 gcggcgaaaa atatcggcac tcagccagtg gcggctcagg tagctgccgc acctgccgca
     2281 gacgtagcag aacaacctca gccgcaaact gcggatggcg ttgccagccc ggcacaagcc
     2341 tcggttagcg atctgaccgg tgatcagcct gcggcccagc cggtgcctgt aagcgccccg
     2401 gcgacaagca ccgcagcggt aagcgcaccc gcaaatccat ccgcagagct gaaaatctac
     2461 gatacctcat cacaaccact tagccagatc ttaagccagg ttcagcagga tggcgcgagt
     2521 attgtggttg gtccgttgct gaaaaataac gttgaagagt tgctgaagag caacactccg
     2581 ctgaacgtac tagcactgaa ccagccggag aatatcgaaa atcgcgtcaa tatttgttac
     2641 ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt
     2701 aaacaagcgc cgctggtgct gatcccacgc agttcattgg gcgatcgcgt agccaatgcg
     2761 tttgcgcaag agtggcagaa attgggcggc ggcaccgttc tgcaacaaaa atttggttcc
     2821 accagcgaat tacgcgcggg tgttaacggc ggttctggta ttgctttaac gggtaccccg
     2881 attactccca gagcgacaac cgactccggc atgacgacca acaatccaac gctgcaaacc
     2941 acgccaaccg atgaccagtt caccaataat ggcggtcgtg tcgatgcggt gtacattgtg
     3001 gcaacgccgg gtgaaatcgc ttttattaaa ccgatgatcg ccatgcgtaa cggtagccag
     3061 agcggtgcaa cgctgtacgc cagctcccgc agtgcgcaag ggaccgctgg cccggatttc
     3121 cgtctggaga tggaaggctt gcagtacagc gaaatcccga tgctggcggg cggtaatctg
     3181 ccgttaatgc agcaggcact cagcgcggtg aataacgatt attcactggc tcgcatgtat
     3241 gcgatgggcg tcgatgcctg gtcgctggca aatcatttct cacaaatgcg ccaggttcag
     3301 ggttttgaaa tcaacggtaa taccggaagc ctgacggcta acccggattg cgtgattaac
     3361 aggaagttat catggctaca gtaccaacaa ggtcaggtag tccccgccag ttaaccacca
     3421 aacagaccgg cgatgcgtgg gaagtacaag cgcgtcgctg gctggaaggc aaaggactgc
     3481 gatttgtcgc cgctaacgtg aacgagcgtg gcggcgagat cgatctgata atgcgtgaag
     3541 gccggaccac cgtttttgtc gaggtgcgtt accgccgttc tgcgctttat ggcggcgcgg
     3601 cagccagtgt gacccgcagc aaacaacaca aattattaca gactgcccgc ttgtggctcg
     3661 cgcgtcataa tgggagtttt gatactgtgg attgccggtt cgatgtggta gccttcaccg
     3721 ggaatgaggt tgagtggatt aaggatacct ttaatgacca ctcataatta aggtttaagg
     3781 attagcgtgc aagaaagaat taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg
     3841 gcggcagagg cgcttccgga tgccatctcc cgtgcggcca tgacgctggt tcagtctctg
     3901 ctcaatggca acaaaatcct ctgttgtggt aatggaactt ccgctgccaa tgcacagcat
     3961 tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc tgccattgca
     4021 ctaaataccg ataatgttgt cttaacggcg attgccaacg atcgcttaca tgatgaagtg
     4081 tatgcaaaac aggtgcgggc gctggggcat gcgggagatg tattgttagc catttccacc
     4141 cgtggcaaca gccgcgatat cgtaaaagca gtagaagctg ccgttacgcg tgatatgacc
     4201 attgtggcat tgaccggtta tgacggcggt gaacttgcag gtttgttagg gccacaggat
     4261 gtggagatcc gcatcccttc gcatcgcagt gctcgcattc aggaaatgca tatgctgacg
     4321 gtaaattgcc tgtgcgatct gatcgataac acgcttttcc ctcaccagga tgattaagga
     4381 gaatacatga aggcattatc gccaatcgca gtccttattt ccgcgctgct gttgcaaggt
     4441 tgtgttgccg ctgccgtagt gggtactgct gctgtgggta ccaaagccgc aactgaccca
     4501 cgcagtgtcg gcacccaggt ggacgatggt accctggaag tgcgcgtgaa cagcgcattg
     4561 tcgaaagacg aacagattaa gaaagaagcg cgcattaatg taacggccta tcagggcaaa
     4621 gtgctgctgg ttgggcagtc accaaacgct gaactttcgg ctcgcgccaa acagattgct
     4681 atgggcgtag acggtgccaa cgaagtgtat aacgagattc gtcagggcca gccgattggt
     4741 ctgggcgaag cgtctaacga tacgtggatc accaccaaag tgcgttcgca gctcttaacc
     4801 agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg
     4861 atggggctgg tgactgaacg tgaagcgaaa gcggcggcag atattgccag ccgggtgagc
     4921 ggcgtgaagc gggtaactac ggcgtttacg tttattaaat agcagcccct tgtaatgcct
     4981 gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg caaaccgtag gtcggataag
     5041 gcgtttacgc cgcatccgac atgttttccc tcaaatcaga ataacagcgc cagcccgccg
     5101 acaatcacgc ctgacactgc caccatcgcc cccgtcagcc ataaggcttt cgccgggaac
     5161 gctttgcgca gcataatcag tgacggcaaa ctcaccgccg ggagcgtcat caacaatgcc
     5221 agcgccggag cagttcccat acctgccagc atcatcgttt gtacaatcgg aatttctgct
     5281 gccgtgggaa tgacaaacaa gcaccctgct accgccatcg ccaccaccca catcaggctg
     5341 ttatcgacag caccatcggc atgggggaat aaccagacgc gagcggcacc caacaccagt
     5401 actgcaagga tgtaaaccgg gatcgtactc cagaaaagcg tccatagcgc cctgccccag
     5461 cgactaaaaa atccgccctg tgcttccggt atgtcaattt cgaccggtgc ttgcgtttgc
     5521 ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca gcaacaccat caccagcccg
     5581 gccaccagac gaatcgccgc aaaaccccag ccgaggacaa accccataaa caccagcgtc
     5641 gccgggttta acaccggatt gcccatccag aatgccagcg caccgcccat cgacacctgt
     5701 tggcgacgca ttcccgccgc gaccggagcc gcacagcagg tacacatcat gcctggcagc
     5761 gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt gcccaagcgt acgcaacaac
     5821 cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga tcacccccag caccgccgct
     5881 ttccataccg cgaggaaata gatcatcgcg taatccaacg ccgcctgcca tggattagca
     5941 tccgcctgcg caaggataga tttaccgata ctgtgggttt cggcagcagt aaacgctttg
     6001 ccgtagtaag gttgccattt cacataccag agaccggcaa tgacaacgag aaagaaaagc
     6061 gcgggtttcc accactgaat gggcgttgcc gcctgagatg aagactgacc agtcatagca
     6121 ttccccagga ataagttatg ataattgagc gcgtgaatat tacgctcact atcaattctt
     6181 gggaataatt attcagctct tttgcgtaat tctgaagagc ttaaaatcgt cacaccttca
     6241 tgctcgggcc gcatcgcctc tgccagcatg acccgcgcca cgtctctcgc atcaatggat
     6301 ttccagttac cgggtaacaa acggaacagc ggcgcaaaaa gcgtttcgtt catccgctgt
     6361 ttgctacgat cgcccagtaa catcgacggg cgagcaatgg tcaatttcgg ccagttctgg
     6421 gcgattaatg cttcttccat ctcccctttg acgcggttat agaaaaacgg cgagtgggca
     6481 ttggcaccca tagcactgac caccagcata tgctgcgcgc ccagtcgccg cccggttaat
     6541 gcggtatcca ctaccagcgt gtaatcggca tgaataaacg cctctttgct ccccgcttct
     6601 cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg gatcggtgac ctgcgccagc
     6661 gcgtcagtca gttgcggatc atggggatta aataccccgg gcatatcgcc caacggacgt
     6721 cgcgtcggcg cggctatggc gttaactttc ggttcgttaa tcaacatccg cagcaggtga
     6781 ccgcccacca gccccgttgc gcctgtaatc agtacctgac tcatcttcgc tcctttacag
     6841 aattgtctgc cttgcgctcc acggctcatg caccaggctt aatagaccat gaggtaatta
     6901 tccccgattg tgggaaattc gcctcatcca atgcaacaac gcggaggaag catgagtaag
     6961 aaaattgccg ttttaatcac tgatgaattt gaggattcag aatttacttc acccgcagac
     7021 gagttccgta aagccggaca cgaagtgatt accattgaaa aacaagcggg taaaacggtg
     7081 aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat ccatcgatga agtgacgcca
     7141 gcggagtttg atgccctgtt gctaccgggc ggtcattcac cggattatct gcgtggcgac
     7201 aaccgttttg tcacctttac ccgtgatttt gtgaatagcg gcaaacctgt tttcgccatc
     7261 tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc gggggcgcaa actgaccgcg
     7321 gttaaaccga tcattattga tgttaaaaac gcgggcgcgg aattttacga tcaggaagtc
     7381 gtggtcgata aagatcagct ggttaccagc cggacaccgg acgatctgcc agcgtttaac
     7441 cgcgaggcgt tacgcctgct cggtgcctga gccgcgtagc caaatcattt ttttaccaaa
     7501 gcccagcgtg ttgtcggtga atttgatttc atcgggccgg atttcccaca ccggggccga
     7561 cagcattctg gcaaccggaa agcgacgatt gtacgccttg cgcgcgaggt cgctttcctc
     7621 accttccagc ctgcggatct cacctttaaa ctgcacgccg cgaattaacg ctaccgtttt
     7681 cggctgaccg tttaccgttc cggcaaccgc agcctgcggc ccactcatct gcgcgtggcg
     7741 cgttttttct tccgtcaaaa tgtagaaggc gactttctgc acatcaaaca gataaaaggc
     7801 attggcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat gttgttttgc
     7861 cagccaacgg ctgatggcgg tgagtgtttc cattgctgtt ctcttttata ctgtgggcct
     7921 gaccttaaca tgcgacgcag acgatgacac cctggtttct ttacttgatc cgtaccgccg
     7981 acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa
     8041 gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg ttttctgcgc
     8101 cagtgggcga tcgttcgctg gcgttacggg cggaatatcg cgttaagcaa ctgaccaaac
     8161 ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc aaagctgtta agcagtctgc
     8221 aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac cagttacgcc
     8281 attcaacgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc ccggccagcg
     8341 gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa cggctgtata gcgccggatc
     8401 gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc cttcatagac
     8461 cagttggcgt gccagcccct gtccacggta tttttcatct accgccagcg gtgccatgcc
     8521 gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat agccaatgac
     8581 ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt cacgcagatc
     8641 gtgaaccagc ttcgcttccg catcactttc gaatgaacga cgcagcaggg catcaatacc
     8701 cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga tgtctgtttg
     8761 gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag cagcatcatg
     8821 cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt cacatacagc
     8881 cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt atccggatct
     8941 tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact cacatcagct
     9001 tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca ttgcaggtca
     9061 atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc accatcatcc
     9121 agcgcctggc ggaattgcca gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat
     9181 ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat acgggaacgc
     9241 agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt gccatatcag
     9301 aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta ccaccaatac
     9361 aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat cccagttcgt
     9421 taacattttt gcgttttgat agcgcaacct tcaggaaaaa ttatggagct gctctgccct
     9481 gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc
     9541 gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa
     9601 ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat cgcgattaac
     9661 acttttgcgc atccggacgg ttacgcccgt tggcagcgtg ccgtggatat ggcggcgcag
     9721 ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tagagtacgc cgccgagcgt
     9781 tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt
     9841 aacttttatc atcgccattt tgacgttgct cgcgtggtgc tgccgcgcgt gttgtcgatt
     9901 catcaggtga aacaactggc acgggtcaca cctgtcccgc tggaagtctt tgctttcggc
     9961 agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt cgtatctgac gggtgagtcg
    10021 cccaacaccg tgggcgcgtg ttctccggcc cgtttcgtgc gctggcagca aacgccgcag
    10081 gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg cgaaaacgca
    10141 ggttatccga cgctatgtaa agggcgttat ctggtggacg gcgagcgcta tcacgcgctg
    10201 gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc ggcgaatatt
    10261 gcttcggtga aaattgaagg ccgccaacgt agcccggcgt atgtcagcca ggtggcgaaa
    10321 gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc
    10381 gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc agaccaccct tggcgcgtat
    10441 caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg ctgtggtact
    10501 ggccaaaaga gacgctggaa gatttttatc agcaggccgc caccagcagc gccgacgtga
    10561 tttatcttgg cgaagcggtt tgcagcaagc gccgggcaac caaagttggc gactggctgg
    10621 agatggcaaa atcgctcgcc ggaagtggta agcagattgt gctctccacg ctggcgctgg
    10681 tgcaggcatc atctgaactg ggcgaactga aacgctatgt tgagaacggt gagtttctga
    10741 ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg
    10801 ccggacacgc gctgaactgc tataacgcgg tgacgctgaa aatattgctc aaacagggca
    10861 tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat ctgcttaatc
    10921 agtgcgatga gctgggcatt cgcaaccagt ttgaagttga agtcctgagc tacggtcatc
    10981 taccgctggc ctactccgct cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg
    11041 agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc
    11101 aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac aacctcggta
    11161 acgagctggc atccatgcag ggcctggttg atgtggtccg cctgtcaccg cagggtactg
    11221 acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgca ccactgccgt
    11281 tgacggcgaa cagcgactgt aacggctact ggcggcggct ggcaggactg gagctgcaag
    11341 cctaagtaaa tagctcactt tgttaacaac tttaactact ctttaatgca gtattaaaga
    11401 ttaatcggta acaaagtgag ctgttatgac tgataaaacc attgcgtttt cgctactcga
    11461 tctggccccc attcccgaag gttcttcagc gcgagatgca ttctcccact ctctcgatct
    11521 cgcccgtctg gctgaaaagc gcggctatca tcgctactgg ctggcagaac accacaatat
    11581 gactggcatt gccagtgctg ccacgtcggt attgattggc tatctggcgg cgaataccac
    11641 cacgctgcat ctggggtctg gcggcgtgat gttgcctaac cactcaccgt tggtcattgc
    11701 agaacagttc ggcacgctca atacactcta tccggggcga atcgatttgg ggctgggtcg
    11761 tgctccgggt agtgaccagc gaaccatgat ggcgctacgt cgtcatatga gcggggatat
    11821 tgataatttc ccccgcgatg tcgcggagct ggtggcctgg tttgacgccc gcgatcccaa
    11881 tccgcatgtg cgcccggtac caggctacgg cgagcaaatc cccgtgtggt tgttaggctc
    11941 cagcctttac agcgcgcaac tggcggcgca gcttggtctg ccgtttgcgt ttgcctcaca
    12001 cttcgcgccg gatatgttgt tccaggcgct gcatctttat cgcagcaact tcaaaccgtc
    12061 ggcacgactg gaaaaaccat acgcgatggt gtgcatcaat attatcgctg ccgacagcaa
    12121 ccgcgacgct gaatttctgt ttacctcaat gcagcaagcc tttgtgaagc tgcgccgtgg
    12181 cgaaaccggg caactgccgc cgccgattca aaatatggat cagttctggt caccgtctga
    12241 gcagtatggc gtgcaacagg cgctgagtat gtcgctggtg ggcgataaag cgaaagtgcg
    12301 tcatggtttg cagtcgatcc tgcgcgaaac cgacgccgat gagattatgg ttaacgggca
    12361 gattttcgac caccaggcgc gcctgcattc gtttgagctg gcgatggatg ttaaggaaga
    12421 gttgttggga tagtgtgtct taacgcggga agccttatcc gagctggcaa cgctgtccca
    12481 cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg cagcagaaat gtcggataag
    12541 gcccgctgat tactgataca ccggcagcaa attaaagctc gataagatat ggaccagtgc
    12601 gttgccaacg ccaaatacca gaatcagcgt aatcatcggc ttgccgcccc agacgcggaa
    12661 tttcgggctg ccaaagcgtt tacgcgatgc gcgggccagc agtgccggaa caattgccgc
    12721 ccagatggta gctgctaaac cagcataacc aatggcgtac aggaatccgt tcgggaacag
    12781 cagaccaccc acaacaggag gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc
    12841 cgagtcgtcg aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccgag
    12901 gaacgaactc gctaccgcaa agtttgagaa cacgaccagc agcagatcca gactacggct
    12961 gttcagtacg ccgcttaacg cctgtaccag cacatcaata ttaccgccct tctctgcaat
    13021 atcgataaac tccggacgcg ggatgttacc catcgtcgcc agcaaccaga tggtatacag
    13081 cgccagcgcc atcagcgtac cgtacaccag acatttcacg atggttttcg gatctttgcc
    13141 gtaatacttc atcaggcttg gcacgttacc gtgataacca aacgatgcca gacagaacgg
    13201 caaggtcatc aacagatacg gtgcataaga cgcattgctt tcagcgacgt tgaacaatgt
    13261 cgcaggctgc acatgcccca gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc
    13321 ccccagtaca atcgctgtca tgcgactgac ggctttagtg ctcaaccaca ccacaaacgc
    13381 taccagcaat gcaaaaccaa aacccgccgc ccgtgccggg acgtttagtg acatctctgc
    13441 gaaggtgtga tgcagaatcg aaccactggc agaaatatag gcataggtca ggatatagag
    13501 cacaaaggca atggaaatgc cgttgaccac gttccagcct ttgcccagca aatctttggt
    13561 gatggtgtca aaactcgaac cgattctgta attcaggtta gcttccagaa tcatcaagcc
    13621 ggaatgcagc atacagaacc aggtaaagat cagcgccgcc attgaccaga aaaaccacgc
    13681 cccggacatg accactggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat
    13741 caccacgccg ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc
    13801 ttctctccag tgaaaaatag tgcgactgcg ttgttatgta ttgcactgta ccagtacgcg
    13861 agtacaaaag acagaaaaaa agccccgatg gttcaaatcg gggctgtata tattatttta
    13921 cagactgtgt tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt
    13981 agagtcatca cgacgcggag cgcggccttc acgacgttcg ccgctaaaac gacgaccatc
    14041 accacgacca ccttcacggc gttcaccgct gaagttacga ccaccttcac gacgctcgcc
    14101 actgaaacca cgaccaccgc cacgacgctc tccgccagta tgcggctgtg catcgcccag
    14161 taattgcata ttcatcggct tgttcagaat gcgagtacgt gtaaagtgtt gcagcacttc
    14221 acccggcata cctttcggca gttcgatggt ggagtgagaa gcaaacagct taatgttacc
    14281 gatgtaacgg ctgctgatgt cgccttcgtt agcaatcgca ccaacgatat gacgaacttc
    14341 aacaccatca tcgcggccca cttcaatacg gtacaactgc atgtcaccaa catcacgacg
    14401 ttcacgacgc ggacgatctt cacggtcgcc acgcggacca cggtcgttac gatcgcgcgg
    14461 accacggtca tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac
    14521 aatcagagta cgttcaccct gtgccatttt cagcagtgcg gcggccagag tttcgagatc
    14581 cagctcttca ccttctgcag tcggctgaat tttgctcagc agtgcgcggt attgatccag
    14641 atcgctgctt tccagctgct gctgtacttt agcggcgaat ttttccagac ggcgtttgcc
    14701 cagcagttct gcgttcggca gttctacttc cggaatagtc agcttcatag tacgttcaat
    14761 gttgcgcagc agacgacgct cgcggttctc aacgaacagc agcgcgcggc cagcacgacc
    14821 cgcacgaccg gtacgaccga tacggtgaat gtaagactca gaatccatcg ggatatcgta
    14881 gttaaccacc aggctgatac gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat
    14941 caggatgtcc agacgaccat ctttcaggcg ttccagtgtc tgctcacgca gcgcctggtt
    15001 catgtcaccg ttcagcgcgg cgctgttgta gccgttacgc tcaagagctt cagccacttc
    15061 cagagtcgca tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag
    15121 gaaacgtacc agtgcttcgt ttttacgcat accccagaca gtccaatagc tctggctgat
    15181 gtcaggacgg gtagtcacgc tggactgaat gcgcacttcc tgcggctctt tcataaagcg
    15241 gcgggtaatg cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc
    15301 ttccgggatc tgcgccataa tagtttcaac gtcttcgatg aagcccatgc gcagcatttc
    15361 gtcagcttca tccagaacca gaccgctcag tttagagagg tccagagtgc cacgtttcag
    15421 gtggtccagc agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg
    15481 taattgcacg tcataacgct ggccgccgta cagggcaacc acatttacgc cgcgcatgtg
    15541 tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgctag
    15601 caccagaatc tgtggtgctt tcagctcagg atcaagattt tgcagcagag gtaaagagaa
    15661 tgctgcagtt tttccgctcc ccgtctgggc catacccagt acgtcgcggc cattcagcag
    15721 atgtggaata cactccgcct gaattggaga tggtttttcg taacccagat cgttaagggc
    15781 ttcaaggata ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta
    15841 gtacgtgtgc ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag
    15901 caattttcat tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc
    15961 ctcacccgtt aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat
    16021 tgctggtccg attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat
    16081 gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc
    16141 gtggcgctgt ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca
    16201 ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac
    16261 gtttgttcgc taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta
    16321 tccgattttt cgaagtgctg tttcaacact tctttagcct gcttctcatc gagcttctgc
    16381 tcggcgagat aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc
    16441 agcagatcat cttgcgctaa cttgtcacga ccgccgtaat ataatgcgat cccgcgattc
    16501 aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca
    16561 gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata
    16621 tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga
    16681 ctatcataca acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc
    16741 cgactggcaa ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc
    16801 aatggtaccg cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta
    16861 agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca
    16921 cggttgaatg aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc
    16981 cccgccgcag cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc
    17041 ggtgctgcag caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc
    17101 tggcgatcaa cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc
    17161 actttctcaa cgcgtttgtc agcgatttga gagatgtgga ccagaccttc tttaccgccg
    17221 ccgatggcaa caaatgcgcc gaagtcaacg atacgggtca ctttaccagt gtagacgcgg
    17281 cccacttcaa tttctgcagt gatctcttcg atacgacgaa tagcgtgttt cgctttctcg
    17341 ccatcggtcg ctgcgatctt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt
    17401 tcttcggtca gggcacggat cacagagccg cctttaccga taacgtcttt gatcttgtcc
    17461 gggttgatct tgatggtatg gatacgcgga gcgaactcag agatatcgcc acgtggcgcg
    17521 ttgatcgcct gttccattac acccaggatg tgcagacgcg cacctttagc ctggttcagt
    17581 gcaacctgca tgatctcttt ggtgatacct tcaattttga tatccatctg cagtgcagag
    17641 ataccgtcgc gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc
    17701 aaaatgtcag acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc
    17761 gcaacggcag ctttgattgg cacacctgcg tccatcagcg ccagagacgc accgcacacg
    17821 gaagccatag aagaagaacc gttggattca gtgatttcag acacaacacg tacggtgtac
    17881 gggaatttgt ccatatccgg cattactgcc agcacgccgc gcttcgccag acgaccgtga
    17941 ccaatttcac gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg
    18001 aagttgtagt ggaacaggaa ggtatcggta cgttcgccca tcagttcatc aagaacctgc
    18061 gcgtcacgag cagtacccag cgttgcggta accagcgcct gcgtttcacc acgggtgaac
    18121 agcgcagaac cgtgagtacg cggcagcacg ccagtacgca catccagacc acggatcata
    18181 tctttttcac gaccatcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt
    18241 ttctcgatcg cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc gtcttcagca
    18301 agcagcgtcg cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg
    18361 tcggtgatgc ggtaagcatc gctcagacga gcttcagcca gtgcagcaac gcgcgcgttc
    18421 agcgcttcgt ttaccggctc cggctgccag tcccaacgcg gtttaccggc ttctttcacc
    18481 agttcattga tgttctgaat aacaacctgc tgttgttcat gaccgaacac tactgcgccc
    18541 agcatctggt cttcgctcag cagttcagct tcagattcaa ccatcagtac agcggcttca
    18601 gtacccgcaa caaccagatc cagtttgctc tctttcagct cgtcctgagt cgggttcagt
    18661 acgtactggt cattgatgta acctacgcgg gcagcaccaa tcgggccatt gaacggaata
    18721 ccagacagag acagtgctgc ggaagcacca atcatcgcga cgatatccgg gttaacctgc
    18781 gggttaacag aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac
    18841 agcgggcgaa tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttggacga
    18901 ccttcacgac ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag
    18961 ttaacggtca gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta
    19021 acaaataccg cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc
    19081 atgccggttt ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta
    19141 agcaatgtaa tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc
    19201 gatcttctgc gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc
    19261 ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt
    19321 ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa
    19381 actcgcaaga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac cagacgacga ggcgcaacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tccgtaacgc gaaccagtcc
    19921 ttccagcggc gcgccagatg tacgaaccgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagcaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 gtgctccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aatcacatgc gcgccacagc cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcatggc gaataaacag
    20281 caattcataa acggtaattg gacgcgcttc acgcggaact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttgccct ggtatttcag tgctgaatac atcgatggga tctgttcgat
    20401 atcgccacgg aaagtatcca gtgccgccgc cagttgttct gcactaaagg ttaccggacg
    20461 ctcttcaacg atttgtccgt cggcgtcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag
    20581 gcaaatcggc aacatgccgg tcgccagcgg gtccagcgca ccggtatgcc cggcacgatt
    20641 ggcgttatat atacgtttca ctttttgcag cgcatcgttg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tacgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttacccagc aggctgcgga tgaaaccaga agcttcctgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa agtcacatac
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 agacgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 gctgttagcc cctttttaaa aattaatttc aggtggaagg gctgttcacg ttggcctgat
    21241 aagacgcgcc agcgtcacat caggcaatcc atgccggatg cggcgtaaac gccttatccc
    21301 gcatggaacc ctaaaaacct taagcgatgg tacgttggat ctcgatgatt tcgaatactt
    21361 cgatcacatc gccagtgcgg acgtcgttgt agttcttaac gccgatacca cattccatac
    21421 cgttacggac ttcgttaacg tcatctttga agcggcgcag ggactccagc tcgccttcat
    21481 agataaccac gttgtcacgc agaacgcgga tcgggttgtg acgtttaacc acaccttcgg
    21541 taaccataca gcctgcgatg gcaccaaatt tcggcgattt gaacacgtca cgaacttccg
    21601 ccagaccgat aatctgctgt ttcagttccg gagacagcat accgctcatc gccgctttca
    21661 cttcgtcgat caggttatag atgacggagt agtaacgcag atccaggctt tccgcttcaa
    21721 tcactttacg tgcagaggca tcagcacgta cgttgaagcc aaccaggatg gcgttggacg
    21781 ccgcagccaa ggtggcgtcg gtttcggtga taccacctac gccagaaccg atgatcttca
    21841 ctttaacttc gtcagtagac agtttcagca aggagtcgga gatcgcttcg acagaaccct
    21901 gtacgtctgc cttcaggacg atattcactt cgtgaacttc gccttcggtc atgttggcga
    21961 acatgttctc gagtttagat ttctgctgac gcgccagttt aacttcgcgg aatttaccct
    22021 gacgatagag tgcaacttca cgcgctttct tctcgtcacg tacaacggta acttcatcac
    22081 ccgcagccgg aacaccggac aagccgagga tttccaccgg aatggacgga cccgcttcca
    22141 gcacttcctg acccagttcg ttacgcatcg cacgaacacg accgtattcg aagccacaca
    22201 gaacgatatc gcccttgtgc agagtacctt cacgtaccag aacggtagca accggaccac
    22261 gacctttatc gaggaaggat tcgataaccg caccgctcgc cataccttta cgtaccgctt
    22321 tcagctccag aacttccgcc tgcagcagga tagcgtccag cagttcatcg ataccggtac
    22381 ccgctttcgc agatacgtgt acgaactggc tttcaccgcc ccactcttcc ggcaggatgc
    22441 cgtactggga gagttcgttc ttaacgcgat ccggatcggc ttctggttta tcgatcttgt
    22501 tcactgcaac caccaccggt acctgcgccg ctttcgcgtg ctggattgct tcgatggtct
    22561 gcggcattac gccgtcgtcg gcagcaacaa ccaggactac gatgtcagtt gcctgcgcac
    22621 cacgtgcacg cattgaagta aacgcggcgt gccccggggt gtccaggaag gtgatcatgc
    22681 cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgggt aatgccgccc gcttcgccag
    22741 aggccacttt cgttgaacga atgtagtcca gcagagaggt tttaccgtgg tcaacgtgac
    22801 ccatgatggt cacaaccgga gcgcgcggtt cagccgcagc tcccgtgtca cggtcgctca
    22861 ttaccgcctc ttccagctcg ttttcacgac gcaggataac tttgtggccc atctcttcag
    22921 caaccagctg tgcggtttcc tgatcgataa cctggttgat ggttgccatt gcgcccagtt
    22981 tcatcatcgc tttgatgacc tgagagcctt taaccgccat cttgttcgcc agttcgccaa
    23041 cggtgatagt ttcgccgatc acaacgtcac ggttaacggc ctgagcaggc ttctggaagc
    23101 cttgctgcag cgaagaacct ttacgttttc cgcccttacc gccacgtact gctgcgcgtg
    23161 cttcttcacg atcagctttt gattcagcgt gtttgttgcc tttcttcgga cgcgctgctt
    23221 tcgcgttacg accacggcca cggccgcctt cgacttcacg atcgctttcg tcttctgcct
    23281 ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga atcctcagtc ggttccgcgt
    23341 tatcagtcca tttgttttct tccgccatac gacgtgcttc ttcagcaacg cgacgtgctt
    23401 cttcttcgag tttacgacgc gcttcttctt cggctttacg cttgagctct gcagcttcct
    23461 gctcacggcg ggctttttca gcctgggcgt ttttagtcat atcgtcttgt tgattgctca
    23521 ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc agcttcacgc ttagcttgtt
    23581 ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt agccgattct tctgcctcac
    23641 gacgggcttg ctcttccgct tcacgctgcg cttgctcttc cgctgcaagg cgttcagcct
    23701 cttgcggatc gcgtttcaca aaggtgcgtt tcttgcggac ttcgatttgt accgatttgc
    23761 tttttccacc ggtaccagga atgttaaggg tgctgcgtgt tttacgttgc agcgtcaatt
    23821 tgtccgggcc tgaatttttc tgattcaggt ggtcaatcaa agtctgtttc tcttgtgcag
    23881 acacagagtc gtcagcagac ttccggatac ctgcatcagc aaattgctgt accaggcgtt
    23941 ccacggaggt ctgtcgctct gcggccagcg ttttaatcgt tacatctgtc atgctgttcc
    24001 ttcctgctac agtttattac gcttcgtcac cgaaccagca aatattacgg gcagccataa
    24061 tcagtgctcc ggctttttcg tcggtcaacc cttcgatatc agccagatca tcaatgccct
    24121 gttcggcgag atcttccagc gtacaaacgc cacgggcggc cagtttgaat gccaaatcac
    24181 gatctacccc ttcaaggttc agcagatcgt cagccggttt gttatcaccg aggctttctt
    24241 cctgggcctg tgcaatggtg gccagtgcat ttttagcacg ctcgcgcagt gcttcaacgg
    24301 tcggctcatc aaggccttcg atttccaaca gctctttcat cggcacatag gccagttctt
    24361 ccagcgtcga gaagccttcc tctaccagaa cagtcgcgaa gtcttcgtcg atgtcgagat
    24421 atttggtgaa ggtgtcgatc gctgcgtgcg cttccgcctg atgcttagcc tgcaggtcgt
    24481 caacggtcat cacgttgagt tcccaaccgc tcagctgcga agccagacgc acgttctgac
    24541 cgttacggcc aatcgcctgc gccaggttac cggcttcaac ggcgatatcc atggtgtgtt
    24601 tatcttcatc caccacgata gaagcaacgt ctgccggtgc cattgcgtta atcacaaact
    24661 gcgccgggtt atcatcccac aggacgatat cgatacgctc gccgcccagt tcagtagaaa
    24721 ccgcctgaac acgcgcgcca cgcataccta cgcaagcacc taccggatcg atacgtttat
    24781 cgttggtttt caccgcgatt ttcgcacgag aacccgggtc gcgagccgct gctttaattt
    24841 caatcacttc ttcgccgatt tctggcactt caatacggaa cagttcgatc agcatttccg
    24901 gcttggaacg agtgacgaac agttgcgcgc cacgcgcttc cgggcgaacg gaataaagca
    24961 cgccacgaac gcggtcgcca gggcggaagt tttcacgcgg cagcatatct tcgcgcagga
    25021 tcacggcttc agcgttgttg cccagatcca gagagatgtt gtcgcggttt acttttttca
    25081 ccacgccggt gatgatttca ccttcgtgtt cacggaactg atcaaccacc atcgcacgtt
    25141 cggcttcacg cactttctgc acgataacct gttttgccgt ctgggtggtg atacggtcaa
    25201 aggtaacaga ctcaatctga tcttcaacgt aatcgcccag gttcaggctt tcatcttcat
    25261 aacgtgcggc ttcaagggtg atttccttgg tcggctgggt gacttcatca acaactaacc
    25321 agcgacggaa ggtgtcaaaa tcaccgcttt tgcgatcgat ctgtacgcgg acgtcgatct
    25381 cttgttcata ttttttcttt gttgctgtcg ccagcgcgct ttccaatgct tcgaaaatct
    25441 tctcgcgagg tagcgccttt tcattggata cggcttcaac tacagccaaa atttctttgt
    25501 tcatcgcggg cttttcacct catccagact attaaaagtg gggaaccaag ttcgccttct
    25561 ggatattact cagcgcgaac acttcatctt taccttcgac ggtaactgtg atcatttcac
    25621 cgtctaccgc tttgataacg ccctgccatt tacgacggtt ttgtaccgcc atacggagaa
    25681 ccagagtcac ctcttctccg acaaaacggg cgtagtgttc agccgtgaac agtgggcgat
    25741 cgagacccgg tgaggagact tccaggttat aagcaacggt gatgggatct tcaacatcca
    25801 gcacagcact tacctggtgg ctcacatcag cacaatcatc aacattgatg ccatcttcac
    25861 tatcaatata gatgcgcagt gtggatgtgc gaccgcgaat aaattcgatg ccaaccagtt
    25921 caaaacccag ggcctcaact ggcgcagtaa tcatctctgt taatttttgc tctaatgtgg
    25981 acaagcccac ccccaagaca taaaaaaagg gcctaaagcc cagttattct gtagtcagat
    26041 aacaaaaaac cccgataaat cggggcttta tataactgaa ccctataacc gcaactgcgg
    26101 tctggagcac tttccagaag gattttttca aatcccacta cgaaggccga agtcttcaca
    26161 gtatatttga aaaaggactc taagggaaag tggttgcggg ggccggattt gaaccgacga
    26221 ccttcgggtt atgagcccga cgagctacca ggctgctcca ccccgcgcct gaaacgtggc
    26281 aaattctact cgttttgggt aaaaaatgca aatactgctg ggatttggtg taccgagacg
    26341 ggacgtaaaa tctgcaggca ttatagtgat ccacgccaca ttttgtcaac gtttattgct
    26401 aatcacgtga atgaatatcc agttcacttt catttgttga atacttttgc cttctcctgc
    26461 tctcccttaa gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa
    26521 gatgattaaa tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg
    26581 ctcatcatca gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc
    26641 ggtaggtcaa cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct
    26701 gtggatgcga caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga
    26761 cgaagaggat tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg
    26821 tctgatcgac tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc
    26881 atttcataac accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt
    26941 gactggtacc atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg
    27001 tagcacctat aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc
    27061 tgaactgcag atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggtcg
    27121 tcatgaaatg tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa
    27181 agcttactcc acggactcca acatgcttgg tgcaacgcat gaagcgaagg atctggaata
    27241 cctcaactcc agcgtcaaaa tcgtcaaccc gattatgggc gtgaaattct gggatgagag
    27301 cgtgaagatc ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtggcgtt
    27361 gaacggtaaa acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg
    27421 tcgtcacggc ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg
    27481 tggtatttac gaagctccgg ggatggcact gctgcacatt gcgtatgaac gcctgttgac
    27541 cggtattcac aacgaagaca ccattgagca gtatcacgcg catggccgtc agttgggccg
    27601 tctgctgtac caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca
    27661 acgctgggtt gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga
    27721 ttattcaatc ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat
    27781 ggaaaaaggc gactcggtgt tctcgccaga tgatcgtatc ggtcaactga ccatgcgcaa
    27841 cctggatatc accgataccc gcgagaaact tttcggttat gccaaaactg gcctgctttc
    27901 ctcctctgcc gcttcaggcg tgccgcagat ggagaatctg gaaaacaaag gccagtaatt
    27961 cgacatcaac cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt
    28021 ctgagcatca cataatcgaa aacatcatgg tttgcatcca caaccagtga gtctccttta
    28081 tagtgtacaa tcacttcctc aagcgtttgc ggctgttccg gctttgttac ccccatccac
    28141 gcattaataa ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtgcgatcc
    28201 acaccatccc caagaacggg gctataccag ataaacatcg gaacatgata tgcctgctgg
    28261 ctggcctccc tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg
    28321 tggtcggcaa aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct
    28381 agcaaactat ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt
    28441 tgaaacacgg cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa
    28501 tgaagaacaa tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc
    28561 aacaacaatt catcaaatcc tctgacataa actgtttcca tggcacgcat ggcgatactg
    28621 gtaactgctg taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat
    28681 cctgcttgat tagccatatt aataatgttg tcggggtaat tatgaatgtc atggctcaaa
    28741 acagaatcag cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc accgcttatt
    28801 gcttgattaa acagtttgat ctgttttctt tgtgcttcga cttgcggtgt cgtagagcgt
    28861 gtatatccat acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag
    28921 gtatcaatac ctgtatccct gactgataat tgaaaatacg gcactgtgtt tgcaattgag
    28981 agtaatcttt gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta
    29041 taggtagcaa atcgcgacgc tagtatatat ggactgaacg catttttatt tttggcatct
    29101 ttataagcaa attgacaagc ggaaaataaa cttcccgaaa tgacaatcag caataatatt
    29161 cctgtcactt tttttgtcgg caaggaaaca tcatatttta ttattacagc caaaaaaaga
    29221 agggataaaa aggcaaaagc acatagataa gggctataca ttcccagcat tttggcaact
    29281 tcatccggat cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag
    29341 gtacaccatg aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg
    29401 gcaacaagca ggcgaagcgg aagggatgat atacgtttaa caaccaggat ggtcagaacg
    29461 aagaacagca atcgctgaac catatgcgct ccggaagagg cgactaagtt cgtaatacca
    29521 aaaacaataa cactaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg
    29581 aatactgtca tccagaaatc caagttcaca accaatataa acaattatca acaatgtgca
    29641 attttatatc agacatgttt ttcaactctg atccatatca gaacgaaaca ctgttagttt
    29701 tttcttttta aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt
    29761 ctctaaacat tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt
    29821 atcgagaagt aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca
    29881 attatgtcag cagtagggaa aagtttataa cgaaggcgta ccggcaccat cgtttcaagg
    29941 taccagctac gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac
    30001 caggaccaaa acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg
    30061 acgggacttg aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca
    30121 attccaccac ctcggcacgg aaactacttt ttagttcggg atatcgctgg tcggcttagc
    30181 cggagcagct ggctgagttt gttcggtttt cgccggcgca ctcaggtttt cccattcgct
    30241 acctttattg gtcttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa
    30301 cgttgccagc aacgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag
    30361 cgtagcggaa gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgtag
    30421 catgatcaga ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta
    30481 cataatcaac ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg
    30541 agtttataat ctttcccact gaagcgggtg tgaatactaa ccaaaacgaa tgaccttcgc
    30601 aagggcaatt ttatcgcatt gtatcaactg cggaaaaaaa cagcaaccga tgtgttcgcc
    30661 gagaaaaaag gcgacaatcg ccgccttttt agccagttat ctaacgcttt aaacggcttt
    30721 tactgcatcg gcgatgcggt gtgcaaattc agtcacctgc gcttcgtctt cgccttccac
    30781 catcacgcga attaacggtt cggtgccgga tttacgcaac agcacacgtc cacggctacc
    30841 cagcgccgtt tcaacttctg cggtcaccgc tttgactgat tcatgctcca gcggatcgcc
    30901 gctacctgcg gtgtaacgca cgtttaccag gatttgcggg aacattttca tgccgctgca
    30961 aaggtcgtgc aggctcatat ggttacgcgc catcgccgcc agcacctgca aaccagcaac
    31021 gatgccgtca ccggtagtgg ttttatccag caggatcaca tgaccggaat tctctgcacc
    31081 gatacgccag cctttctcct gcattttttc cagtacgtag cggtcaccca ctttcgcgcg
    31141 cgcaaatgga attcctaact gtttcagcgc cagttcaagc cccatgttgc tcatcaatgt
    31201 acccacagcg ccaccacgca gctggccctg acgaagacct tcacgcgcga tgatatacat
    31261 gatctgatcg ccatcgactt tattgccttc atggtcaacc ataatcacgc gatcgccgtc
    31321 gccgtcgaag gcaataccga gatccgcttt ttcagccagc acacgagcct ggagcgcgcg
    31381 aacgtcggta gcccccactt cggcattgat gtttacaccg tttggctcac aaccgatagc
    31441 gataacattc gctcccagtt cgcgcagcac gttcggcgcg atgtgataag tcgcaccgtt
    31501 tgcgcaatcc accacaatct tcagttcact gaggctaagt tcgttcggga acgtggcttt
    31561 gcaaaactcg atatagcgac ccgcagcatc aacgatacgg ctggctttac ccagttctgc
    31621 cgaatcaaca cagctgatct ctttttccat ttccgcttcg atggcttctt caactgcatc
    31681 cggcagctta gtaccgtcaa tagaaaagaa tttaatgcca ttatcgtaga acgggttatg
    31741 cgatgcagat atcacaattc cggcctctgc gcggaaggta cgcgtcagat aagccacggc
    31801 cggtgttggc atcgggccag tgaagagtgc ggaaaggccc gcagccgcca gccccgcttc
    31861 cagtgctgac tccagcatat agccagaaat acgcgtgtct ttaccaataa taatcttacg
    31921 ggagccgtgg cgcgccagca ctttacccgc ggcccaaccc agcttaagca caaaatcagg
    31981 tgtgatcggc gcatccccta cacgaccacg aatcccatcg gtaccgaaat atttacgatt
    32041 actcatagcg tttgttttcc tttgcagaca gagtagcttc caccactcgc atcgcttcta
    32101 cggtttcttt gacgtcatga acacgaataa tgtgcgcgcc ttgcattgcg gcaatgaccg
    32161 cacaggccag actaccgctc aggcgctcgg acggccccac gttcagcagc tgcccaatca
    32221 tcgattttcg tgacataccc accaacagcg gcaggttgaa atggtgaaat tcagccaggc
    32281 gcgccagtaa tgaatagtta tgggagagat ttttaccgaa accgaatccg gggtcgagca
    32341 acaatttctc ttttgcgata cccgcctgct cgcaacgtgc tatttgctca ataaagtagc
    32401 gattcacttc tgcaaagaca tcgtcatact tcggggcttc ctgcatggtt tttggatttc
    32461 cctgcatatg catcagacaa accggtaaac cggtttctgc agccgcctcc agagcgccag
    32521 gttcggaaag ggagcggata tcgttaataa tgtgagcgcc aactttcgct gactcacgga
    32581 tgacttctgg tttggatgta tcgaccgaga tccagacttc gaagcgttga gcaattgcct
    32641 caaccacagg aataacacgt tgcaactctt cttcaacgct aacttccgcc gcccctggcc
    32701 gcgtggactc gccaccaacg tcaatgatcg tcgcgccagc gttgatcatc agattcgcat
    32761 gtttcaccgc atctatcagc gagttatgcg tgccaccatc cgaaaaggaa tcaggcgtga
    32821 cgttgaggat ccccattacg tgaggatggc taaggtccag tgaagtaccc tgggcaaaga
    32881 gtttcatgat gttatccctg gtatgaattg ataagaaaaa accccggagc acgccccggg
    32941 gttttcggta caaatacagt catctgatgc gggaacttac ttgtcgccta actgctctga
    33001 catggtgtta cccgggttcg gcgtacgcgg ttcatcaacc ggacgaggag cctttggact
    33061 accattgtcg ccagcattgt tagaagcgcc tggttcttcc cagcccgctg gcggacgtac
    33121 atcgcgacgt gccatcaggt catcaatctg cggtgcgtcg atagtctcat atttcatgag
    33181 agcatctttc atcgcatgca gaatatccat attgtcggtc agaagctgac gcgcacgatt
    33241 atagttacgc tcaatcagtg ctttcacttc ctggtcgatg atacgtgcag tttcatcgga
    33301 catatgtttc gctttcgcta cgctacggcc gaggaacact tcaccttctt cttccgcgta
    33361 cagcagtgga cccaatttct cagagaagcc ccactgagtc accatgttac gtgccaggtt
    33421 ggtcgcaact ttaatatcgt tggacgcacc ggtagaaaca tgttccggcc cgtagatgat
    33481 ctcttctgcc agacgaccac cgtacagcgt agaaatctgg ctttccagtt tctgacggct
    33541 ggcgctgatt gcgtcgccct caggcaagaa gaaagtcaca cccagcgcac gaccgcgtgg
    33601 gataatcgtc actttgtgca ccggatcgtg ctccggcacc aggcgaccga taatcgcatg
    33661 acccgcttcg tggtaagccg tcgattcttt ctgcgcttcc gtcatcacca tggagcgacg
    33721 ttccgcaccc atcatgattt tgtctttcgc tttctcgaac tcaaccatcg acacaacgcg
    33781 tttgttgcca cgagcagcga acagtgccgc ttcgttcacc aggttcgcca ggtcagcacc
    33841 ggagaaacca ggagtaccac gggcaatgat tgccgcgtcg atatcgggtg ccaatggtac
    33901 gcgacgcatg tgaaccttca ggatctgctc acgaccgcga acatctggca agccaaccac
    33961 aacctgacgg tcgaaacggc caggacgcag cagcgctggg tcgagaacgt ccggacggtt
    34021 agtcgcggcg ataacgatga taccttcgtt accttcgaag ccatccatct caaccagcat
    34081 ctggttcaga gtctgttcac gttcatcgtg accaccgccc agacctgcgc cacgctggcg
    34141 gcctacggcg tcgatttcat cgataaagat gatgcacggt gccgctttct tcgcctgttc
    34201 gaacatgtca cgaacacggg atgcacccac accgacgaac atttctacga agtcagaacc
    34261 ggagatagta aagaacggaa ctttcgcttc gcctgcaatc gctttcgcca gcagcgtttt
    34321 accggtaccc ggaggaccga ccatcaggac gcctttcggg atcttaccgc cgagtttctg
    34381 gaagcggctc ggctcgcgca gatactcaac cagttcagca acttcttctt ttgcttcgtc
    34441 gcagcccgca acgtcagcaa aggtcgtttt gatctgatct tccgtcagca tgcgcgcttt
    34501 actcttacca aacgacatgg cacctttgcc accgccgccc tgcatttgac gcatgaagaa
    34561 gatccagaca ccaatcagca acagcatcgg gaaccaggag atgaaaatag aagccagcag
    34621 gctgggttct tcaggcggtt caccgacaac cttgacgttc ttggtcaaca ggttatccag
    34681 taatttcgga tcctgaaccg gaatgtaagt ggtataacgg ttactatctt tcttggtaac
    34741 gttgatttca cgtccgttga tacgcgcttc acgaacctgg tcgttattga cctcttgtag
    34801 gaaggtagag taatccacct tacggccatt agactcgctg ggcccaaagc tctggaatac
    34861 tgacatcagc acaacggcaa tgaccagcca gagtattagg tttttcgcca tgtcactcaa
    34921 gggattaacc tcttattaca actgtgttaa aaacagcgtc aggatactct atatccagca
    34981 tctttcaaac tttcgtctga aatctcccgg ttagggttta cgcccggtcg ctacaatata
    35041 cacttcccgc gaacgtgcac gagaagagtc cggcttacga actttgacct tcgtaaacag
    35101 ggagcgaatt tcccttagat actcatcgaa accttcgccc tggaacacct tcactacaaa
    35161 actgccacct ggcgctaata catcacgaca catttctagc gccagttcca ccagatacat
    35221 ggcacggggg atatccaccg ccggtgttcc gctcatgttt ggtgccatat cggacatgac
    35281 aacctggact ttgctgtcgc caacgcgctc cagcagtgct ttcatcacca gttcatcacg
    35341 aaaatcgccc tgaagaaagt ccacaccaac gataggatcc ataggtaaaa gatcgcaagc
    35401 gatgatgcgg cctttgccgc caatttgggt gaccacatat tgtgaccaac cacccggagc
    35461 agcaccaagg tcgacaaccg tcattcccgg tttaaagagt ttgtcacttt gctgtatttc
    35521 atcaagttta aaccaggcac gggaacgtaa cccctttttc tgtgcctgtt gaacatattt
    35581 atcgctaaag tgttcctgaa gccagcggct ggagctggca gaacgcttct tacctgtcat
    35641 ttaactttcc catggggata actcatcgta accaattgcg taaattttta cgcgcctatt
    35701 tggtgatata tgggagatgg cggtagaatg acccgttttc aatcccaacg taagcaaaaa
    35761 atacgatgaa tctgagtact aaacaaaaac agcacctgaa aggtctggca catccgctca
    35821 agccagttgt tctgcttggc agtaatggtt tgaccgaagg ggtgctggcc gagattgaac
    35881 aagcgttaga gcaccatgaa ctcatcaagg tgaaaatcgc caccgaagat cgcgaaacta
    35941 aaaccttgat cgtggaagct atcgtgcgcg aaaccggcgc ctgtaatgta caggtcatcg
    36001 gtaaaacgct ggtgctttat cgcccaacta aagaacgtaa aatctcgctg ccacgctaag
    36061 attatcctaa agttacacac atttgctgtg taaaacgagg ggttttccgc aggcaggaga
    36121 gcaaaatgcc acgctctgtt cgttgataaa aggccgctat gcggcctttt tcctttcttt
    36181 acaatacatc aacatcttga gtattgggta attcttacag gtattccacc ttaattactt
    36241 caaattctac ttcgccgccc ggcgttttga tgaccacaac atcatcttct tctttgccga
    36301 tcaggccacg agcaataggt gagtttacag aaatcaggtt ttgtttaaag tcagcttcgt
    36361 catcgccaac gatgcgataa gtctgttctt cgtcagaatc gagattcagc accgttacgg
    36421 tagcaccaaa aataacgcgc ccattgttgg gcattttggt gacatcaatc acctgcgcgt
    36481 tcgacagctt ggcttcgatg tctttgatac ggccttcgca gaagccctgc tgttcacgag
    36541 ctgcgtggta ttcggcgttt tctttcaggt cgccatgctc acgcgcttcc gcgatagcag
    36601 caatgatttc aggacggcgc acagatttca gaaaatccag ctcttcgcgt aatttttcag
    36661 cgccgcgtaa ggtcatcgga atagcttgca tttgttatac ctcttgaata ttcctgatgg
    36721 ggcaagtgtt cacccacccc ggctgttcgg cctgcccggc ataactcctc accgggacca
    36781 gaagcaaaaa aataccgacc cgggtacaag tcccaggtca gctacaattc acattttgat
    36841 agtcatttta ccctgaagtt cccgaagggt catcgtttac tttatagggc gttgcgccgt
    36901 agtatgacgg ctcgattcca ggttgttagc gcgagattat gcgattttcc agatttatca
    36961 tcggattgac cagctgtata gcgttcagtg ttcaggccgc aaatgttgat gagtacatta
    37021 ctcaactccc cgctggtgcc aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc
    37081 ccgctattga ttaccacagt cagcagatgg cgctgcccgc cagtacccag aaagtgatta
    37141 ctgcgctggc ggcgttgatt caactcggcc ccgattttcg tttcaccacg acgcttgaaa
    37201 ccaaaggcaa tgtggaaaac ggcgtactta agggtgactt agtggcgcga tttggtgccg
    37261 atccgacgtt aaaacgtcaa gatattcgca atatggtcgc gactttgaaa aaatctggcg
    37321 tcaaccaaat cgatggcaat gtgttgatag atacctccat tttcgccagc cacgataaag
    37381 cccccggctg gccatggaat gacatgacac aatgctttag cgctccgcct gccgtcgcca
    37441 tagttgaccg caactgtttc tccgtctcgc tctacagtgc cccaaaacct ggtgatatgg
    37501 cttttatacg cgtggcatct tattaccccg ttacgatgtt cagccaggta cgcaccctcc
    37561 cccgtggttc tgccgaagcg caatactgcg aactggatgt ggtgccaggc gacctgaacc
    37621 gctttacgct gacgggatgc ctgccacaac gttctgagcc gctcccgttg gcttttgccg
    37681 tgcaggatgg agccagctat gccggtgcaa ttctgaaaga tgagttaaaa caggcgggta
    37741 tcacctggag cggaacactg ctgcgccaga ctcaggttaa cgaacctgga acggtagttg
    37801 ccagtaaaca gtcggccccg ctgcacgatc tgcttaagat tatgctgaaa aagtcggaca
    37861 acatgatcgc cgatacggtt ttccgcatga taggccatgc gcgcttcaat gtgcctggaa
    37921 catggcgggc cgggtcggac gctgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata
    37981 ttggaaacac cattattgcc gatggttcag ggctttcgcg gcataacctg attgcccccg
    38041 ccaccatgat gcaggtgctg caatacattg cccaacacga caatgaactt aactttatct
    38101 ccatgctgcc gctagcgggc tatgacggct ctttgcagta ccgtgcaggt ctgcatcagg
    38161 cgggcgtgga tggaaaagtc tcagcgaaaa ccggttcgtt gcagggggta tataacctgg
    38221 cgggattcat taccacagcg agcgggcaac gaatggcgtt tgtacaatat ctttctggct
    38281 atgcagtaga accggcggat cagcgtaatc gccgtattcc gttagtgcgt tttgaaagcc
    38341 gtttgtataa agatatttat cagaacaatt agtcaaaaag aaaccccggc acatggccgg
    38401 ggttttgctg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag
    38461 tcgtcatccc agtcctcatc atcctcttca gcaatctctt caagctgctg gcgatgataa
    38521 tcatcccaca tgaattcgac tttctctggc tgtttcgctt cttcagcctg cacgaccggg
    38581 ttttcaatga taaaggtcat cacatcccag cagagatctt tcacgcccag tccactcgcc
    38641 gcagagatca gataatattt atcttcccag cccagcgcct ctgcgatcgc tttcgctttc
    38701 tcttcggctt ctaccttatc cagcagatcg atcttgttga agactaacca acgcggtttc
    38761 gccgccagat cctggctgta tttttccagc tcgctgataa taatacgcgc gttttcaacc
    38821 ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca acaggacgcg gcaacgttcc
    38881 aggtgcttca ggaagcgaat gcccagacct gcgccttccg cagcgccttc aatcagtcct
    38941 ggaatatcgg caacaacgaa gctcttttcg ttgtccattc gtaccacacc cagacttggc
    39001 accagagtgg taaacggata atccgccact ttcggtttag ccgccgatac cgcacgaata
    39061 aaggtcgatt tacccgcgtt tggcatcccc aacataccga cgtcagccag cagcatcagc
    39121 tccagcagca gctcacgctt atcgcccggc gtaccgttag ttttctgccg tggtgtacgg
    39181 ttaactgacg atttgaaacg ggtattgccc agaccgtgcc agccgccctt agcaaccagc
    39241 agacgctgac cgtgtttggt catatcgccc atggtttcac cagtaccctg gtcgattaca
    39301 cgcgtaccta ccggcacttt aattgtgacg tctttaccgc gcttaccggt acagtcgcgg
    39361 cttgcgccat tctgaccgcg ctctgcacgg aaagattttt caaaacgata atcgataagc
    39421 gtgttcaggt tctcgtcggc ttccatccat acgtcaccac catcaccgcc gtcgccgcca
    39481 tccgggccgc ctttcggaat atacttttcg cggcggaagc tcacgcaacc attaccgcca
    39541 tcacctgcaa cgaccagaat cgatgcttca tcaacaaact tcattttatt ctccgtaaat
    39601 cattcgcctg cgcggggttg cgataccacc gttgtatgct tacgtaatcc gccccaaata
    39661 cgatgaccaa tggcggaata catcgcgccc gcaaccacga caaacgcacc gagataacct
    39721 aaaaggttta acatcggtct ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa
    39781 aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag
    39841 cgagccatcg cttccgccag ggcgccatat cctaccaagg tattcagtcc gcaaaaaatt
    39901 aaacatgcga gctgccagtg gctaagctgc gctatcactc caggctttgc cagagggaag
    39961 agcgcaattg tacataaagt gtacagtaaa aacaggatct gcggtgaggc cagccgacgc
    40021 aataaaacct tttgcgccac gccataactc acccaaaccg tcgcagcacc gaccccaaag
    40081 ataactcccc aggtgtaatc ggttagcttt gtaaatatct cgaccagact ggtgttaaaa
    40141 aacatcacca ggccgctcag gagcatcaat gcccctacaa cctgagtgct gcgcattttc
    40201 tctttcagga taaatacgct ggcaaccatc atgccaactg gcgagagttg cccaatcacc
    40261 tgcgaagcgg tcggactcag gtattgcaag gatgagctga acaggatgaa gttcccaaac
    40321 agcccggcgg tcgccactgc caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat
    40381 ggcggcaacc tcttcttcac cgcaagaatg gcccccaggc caatactcgc catcaagaaa
    40441 cggtaaaaca cgattgtcgg aggttccatc acctccagca cctgcttcat tgcaattggc
    40501 aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc tgcctgctgc
    40561 ttcataccgt attccctgca aagcgcattt gcggattaac gtcgcaccat gcgcattttt
    40621 accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg
    40681 acgcgaaaaa cttattcagc ttcgatgctg ataaatttac ggtttttcgg gcctttaact
    40741 tcgaatttca ctttaccgtc tgctttagca aacagagtgt ggtcacgacc gcaacctacg
    40801 ttagcgccag cgtggaattt ggtaccacgt tgacgaacga tgatgctacc cgccagaacg
    40861 gattcgccac cgaaacgctt aacgcccagg cgtttagctt ctgaatcgcg accgttacgt
    40921 gtggagccgc cagccttttt atgtgccatt tgaaatctct cctcaggtct taggcgctga
    40981 tgccagtaat tttcacatca gtgaaccact gacgatggcc ctgctgctta cgatagtgtt
    41041 tacgacgacg aaacttaacg attttaactt tctcgccacg accgtgagca acaacttcag
    41101 ctttgattac gccgccatca acgaaaggaa cgccgatttt gacttcttca ccgtttgcga
    41161 tcatcagcac ttcagcgaac tcaacagttt cgccagttgc gatgtccagc ttttccaggc
    41221 gaacggtctg accttcgctt actcggtgtt gtttaccacc actttggaaa accgcgtaca
    41281 tataaaactc cgcttccgcg cacacctttt caatgattca gagtgcgcta taaatattca
    41341 caatagggcg cgaatattac gcaaaacgca cgcctttgac aagtgctaca gtcaatacac
    41401 gaagaaaaaa aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatc
    41461 accctatatt cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc
    41521 gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat
    41581 ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca
    41641 gttaggctat tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact
    41701 ggctgcacga gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga
    41761 gtttatccac acggcgactc tgctgcacga cgacgttgtg gatgaatcag atatgcgcag
    41821 gggtaaagct accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt
    41881 tatttatacc cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt
    41941 catgtcagaa gccgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaatgttaa
    42001 cgatccggac atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct
    42061 gtttgaggct gccgcgcagt gttccggaat tctggcgggc tgtacgccgg aggaggagaa
    42121 aggcctgcag gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact
    42181 cgattacaat gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg
    42241 taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat
    42301 gatccgtacc gccatcgaac agggtaacgg tcgccatctt ctgaaaccgg ttctggaagc
    42361 aatgaacgct tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa
    42421 agccatcgca gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct
    42481 cgcgcacatc gctgttcaac gcgatcgtta atcccctccc ctcatcccgc gcagtgcgcg
    42541 ggatgattcc agtaaactcc ataagaatac ctattcagcg taataatgca ctgaaaaacc
    42601 agtgcataca tctttatata ttctgaatat tcacactctt tataggaact ttttagagca
    42661 atcggccatc aggagtatag tgatgctcga cagaagaagt gttctgaatg aaagcgacta
    42721 acttaaggag tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat
    42781 tgcgggtttg cgcaagaagg gaacatcaat ggcggcggag tctcgcagaa atggtttgag
    42841 ttcctcaacg ctggcgaatg cattatcgcg cccatggccg aaaggagaga tgattattgc
    42901 gaaagccctg ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac
    42961 ccatgagttt atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt
    43021 ggtctggcgg tagccccgcg aacggggctg ccagctctca gacgattatt cgcctttcac
    43081 acgctcaata tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt atccacgatc
    43141 gatgtgataa atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag
    43201 gcttgctgat gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc
    43261 gtgacaaata acggtattgc tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg
    43321 cacatgcata aagcggtttt caaagaccgt ttcggtgatg aatccagtcc cttctgccac
    43381 caggttcaac agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt
    43441 acgtacgtta acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc
    43501 gacctcgatg tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg
    43561 ctgcgtgtta cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc
    43621 ggtttcgata cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc
    43681 gatgacgata cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt
    43741 cgcggtatcg acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc
    43801 cagggttgca gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat
    43861 atgtgcgcct ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat
    43921 ggtcgcgcct aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt
    43981 acaaccgcca ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc
    44041 ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc
    44101 gcgggcatca atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag
    44161 cttcattgat gtatcgacgt ctttcagttt cgggacgttc tggatctcta ccggttcttc
    44221 cgccagtaga gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac
    44281 ttcgccctgg agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag
    44341 ttaacaattc atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg
    44401 atcgcgcgcc cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc
    44461 cgcaatatat tccatcagcg gaccatagac cgtctgctgt tttttaaccc gactcatgcc
    44521 gtcaaacaac tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac
    44581 ttcctggagg gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc
    44641 ttcaatcatc agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat
    44701 aagcaaaaag cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac
    44761 aggcggttaa cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa
    44821 attatacaat ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt
    44881 gccctgcttt ttcgccagat cgataagatg gagcagcagt gccagtccac ccgtatccac
    44941 gcgggacaca cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc
    45001 ccaaagcggt agcagaacgt cctgatccag ctctccggat aacgccagtg tgtcacccgt
    45061 ctgcatccag ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga
    45121 gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt tggtacgtag cagcgttccc
    45181 cactcgtttt gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc
    45241 cagttgcccg tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca
    45301 ttcgggtcaa taatggtaac gcgaataggc acaatggttt tatcacccag cggctgttct
    45361 ggcgcaatct gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc
    45421 aggtactcac ggaaagcggc aaagtaagca tcacgttgag ctggggtcgc actcttgtaa
    45481 tactggccca gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga
    45541 tcaacaatgg tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg
    45601 cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat acggattggt ctggtctgcc
    45661 gcggttgccg cactcagcgg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac
    45721 atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta
    45781 cctggcgcag cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa
    45841 aggaactgac caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg
    45901 ccatccttca ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga
    45961 tattgttccc ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatatgg
    46021 ttataacgtt gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta
    46081 atatccgcca cccggcccac aacaacgcca ccaatactga ccggagagcg cgctttcagg
    46141 ccgccaatgt tatcgaacgt agcataaagc gtgtaggtcg gttcagtacg tattgacgtc
    46201 acgttcgccg ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata
    46261 cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt
    46321 cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc
    46381 ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt
    46441 caccgtgatg gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc
    46501 gacggcattt tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc
    46561 gaccagcgat ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga
    46621 aataacccca gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat
    46681 ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga
    46741 accagcacgc ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga
    46801 taacgccacc agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc
    46861 ttgcagcccc aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag
    46921 gacgccgaca ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt
    46981 gccgaccagc gcattgaata acattaaccc ggcccgccca aacgttctca gggttttaat
    47041 ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta
    47101 aaagatcagc gtgataatcg ccggcaggat agcggaacgg aaccggcccg tcagctatcc
    47161 cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat
    47221 gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg
    47281 gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca
    47341 gcttcaccag tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga
    47401 tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg
    47461 aaagttcaga aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca
    47521 tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga
    47581 cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc
    47641 gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa
    47701 ggatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac
    47761 cgatgcccga tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat
    47821 tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg
    47881 ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca
    47941 atttaacctt gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg
    48001 catttgtttt actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga
    48061 aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg
    48121 gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc
    48181 gcgtctattc tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc
    48241 agtattggta catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa
    48301 cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc
    48361 ggtctggctg cgctggttcg tccttttacc gtccattccg atgttctacg ccgtgaatta
    48421 cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt
    48481 cgcagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa
    48541 cttgcacgtc aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag
    48601 ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc gcttatcatc
    48661 atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa ttactttgcc
    48721 atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg
    48781 gcaaccgcaa tagcgggcgt tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt
    48841 ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga
    48901 gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt
    48961 tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg ggtattatta
    49021 actggcggat ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa
    49081 taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc
    49141 aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa
    49201 tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat
    49261 ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg
    49321 tacaccttca tttttcgtcc atcctggtga agccgcgcat ggtgatttag gcatggtcac
    49381 cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcactgcctt
    49441 aattccagtg cttaagcgtc ttcacatacc gttaatctgc atcaccggtc gcccggagag
    49501 cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc
    49561 gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg atgccctcgc
    49621 tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg
    49681 cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga
    49741 gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag ttacccgcaa
    49801 aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga
    49861 cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga
    49921 tgtgatgacg ccggggggaa tacgcgtgcg ccctggcatt ctggccgttg aggcactgaa
    49981 cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg
    50041 tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca
    50101 acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt
    50161 atagcaaaag cagagaacat tcgtctgcta atcctcgacg ttgatggcgt actgtcagat
    50221 ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt
    50281 tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag
    50341 gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag
    50401 tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc cccggaaaat
    50461 gtggcttatg tcggcgatga tctcatcgat tggccggtaa tggaaaaagt gggtttaagc
    50521 gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt gacgcgcatt
    50581 gctggcggtc gtggcgcagt gcgagaagtt tgcgacttat tactcctggc gcagggcaaa
    50641 ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc
    50701 tatcactggc ggttctggtg atgatcggta ttaatatggc cgaaaaagac gataccgccc
    50761 aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct
    50821 ataacccaga aggggcgcta agctatcgat tgattgctca acacgttgaa tattattccg
    50881 atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc
    50941 cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat
    51001 atggacacgt tgaagtcaac gcacttgtgc cggactctca acttcgcaga atcacgacgg
    51061 ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc gtcacgttat
    51121 acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg
    51181 ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt
    51241 aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga
    51301 gacactgatc agccgataca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac
    51361 gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccataaaaat taatgccgac
    51421 aaagtggtcg ttacccgtcc gggtggcgaa caaggtaaag aagtgattga cggctacggt
    51481 aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc
    51541 cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag
    51601 caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga gcagaaaatg
    51661 caggctttca gcgacaaagg caagcgtgta accaccgttc tggtgccgtc gcagctgcag
    51721 gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca
    51781 acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc
    51841 agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag
    51901 accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa catcattatt
    51961 gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat
    52021 ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta
    52081 ctgcaaattc gtgacgactt gtctgctgaa cagcgtgaag accgcgcgaa cgagctgatg
    52141 gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa
    52201 cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac
    52261 gaaccgtttg ccggggttga cccgatctca gttatcgaca ttaaacgcat cattgagcac
    52321 ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg
    52381 gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca
    52441 gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga
    52501 tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt
    52561 tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc
    52621 tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc
    52681 cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg
    52741 aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg
    52801 atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca
    52861 ttgacgacga gctgccggtc tatcagggcg aaacgacgca gaccttgcag gattacctga
    52921 tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg
    52981 tcgatgccgt tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta
    53041 tgggcgatga agagatcgac atcgacgaag ttgaagccgt ccttaagcgg atccaacggt
    53101 ttgatccggt cggtgtggcg gcaaaagatc tgcgtgactg cctgctgatc caactctccc
    53161 aattcgataa aaccacgccg tggctggaag aggccagact gatcattagc gatcatctcg
    53221 atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg
    53281 tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg caatcgatcc
    53341 agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact
    53401 ggacggtaga actcaacagt gacagcattc ctcgtctgca aatcaaccag cactacgcct
    53461 cgatgtgcaa taacgcgcgc aatgatggtg acagccagtt tatccgcagc aatctgcaag
    53521 atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc
    53581 gctgtatcgt tgaacagcag caagccttct ttgagcaagg cgaagaatat atgaaaccga
    53641 tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata tctcgcgtga
    53701 ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca
    53761 gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt gcgctggtga
    53821 agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt
    53881 tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt
    53941 tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac
    54001 actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt
    54061 gttacagcca aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt
    54121 gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt
    54181 gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat
    54241 aagctggcac gccagttgac caaacataaa gataaactga aacaacacta attgtccggg
    54301 caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg
    54361 ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat
    54421 gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac
    54481 tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc
    54541 ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt
    54601 atcggcaatg gtattgccat tccgcatggc aaactggaag aagatactct gcgtgccgtt
    54661 ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggtg
    54721 gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg
    54781 tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag
    54841 agcgatgaag agctgtatca aatcattacg gataccaaag gtactccgga tgaagcgtag
    54901 ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc
    54961 gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt
    55021 ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg aaccctggcc
    55081 gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa
    55141 atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg
    55201 gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt
    55261 tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgatct gctggagcct
    55321 ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggca
    55381 gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat ggtctttgag
    55441 tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc
    55501 ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga taagcctgtc
    55561 gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat
    55621 cttgagctat ggttacctat gctggaaacc aacaaccgta gctacttgac ggtcgccatt
    55681 ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc
    55741 cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg taaaccatga
    55801 ctgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga
    55861 agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca
    55921 ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc
    55981 agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc gttatcgccc
    56041 tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca actcccttca
    56101 aactcccccc gacaataaga aaatcacgta ctgaaatcgt tctcaatcaa cgtcaattgt
    56161 acatttcgtg cgctttttac tttcagaaga accttaagaa aaccttaaga agcattgttt
    56221 aggtttcgtt taagttaatc gaccatactg gagatcgtca gaaaatattt ccaggagatg
    56281 gcatgattcg cttatctgaa caaagtcccc tgggcaccgg aagacatcgg aaatgttatg
    56341 cgcatccgga agatgcccaa cgctgtatca agattgtcta ccatcgtggc gatggtggcg
    56401 ataaagagat ccgtcgtgag ttaaagtact acgcgcatct tggtcgccgg ttaaaagact
    56461 ggagtggaat accgcgctat cacggtaccg tcgagacgga ttgcggaacc ggttatgttt
    56521 atgatgtgat tgctgatttt gacggcaagc cctccattac gctgaccgaa tttgctgaac
    56581 aatgccgtta cgaagaagac gttgcacaac tgcgccaatt actgaaacag ctgaagcgtt
    56641 atttgcagga taaccgtatc gtgacgatgt cgctgaagcc gcagaatatc ctctgtcacc
    56701 gcattagtga gtcagaggtt atccccgtcg tctgcgacaa cattggtgaa agcacattga
    56761 ttccactggc gacctggtca aagtggtgtt gtctgcgtaa gcaagagcgg ttgtggaaac
    56821 gatttatcgc ccaaccggcg ctggctattg ccctgcagaa agatctgcaa cctcgtgaga
    56881 gcaaaacgct ggccctcgct tcgcgcgaag cttaatccag ctggtgttgt tgcataaacg
    56941 gctcaccgcc taactgatac atctgccgta aaatccacgc ctgacggctg cgcacgtagc
    57001 ccgatggcgc ggagactttg aaacgaagtg gattaggtaa tacagctgcc agtaaggcag
    57061 cttccgatcg ggtaagtttg ctcgcgggtt tgtggaaata acgttgtgcc gcagcttcga
    57121 cgccaaacac accgtcgcca aattcggcga tattcaggta aaccgtcagg atacgctttt
    57181 tgctccagac agtttctatc cccagcgtta atccggcttc cagccctttt cgaacccagc
    57241 tacgcccatc ccataaaaag agatttttgg ctgtctgttg agaaatcgtt gaagcaccgc
    57301 gaatacggtt ttcattgcgc tcattgtgcg ccagggcttt ctcaatggaa gcgacatcaa
    57361 agccccagtg ctcaggaaat gtctgatctt ctgcggcaat caccgccagt cccatccacg
    57421 gcgagatttg atccatactg accctgtcag aatgtgccac gtaacgaaaa ttaccatgca
    57481 gccaggcgct gacctgtcgc tcgaccatta ccgcagagaa gggaacaggt gcaacgctaa
    57541 acaacgcgat gcccccgccc cagaaaacgg cgaggacaac cattaaccgc agaagaaaac
    57601 ggcgaacgaa actaaacacc gttaagcggc ttttactcat tcagccagaa ccagcacgcg
    57661 ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg ttctgcgcca gcatatatgc
    57721 cggggtggtg acaatcttat tgtcttcatc aaccacgata tcatcgacag gacacggcac
    57781 atgctccgcg cccatctctt ccagcacttc tgcggtatcg atatcagtac cgatggtcaa
    57841 acgcagcggg aaatcgaaaa ttttcggcag catcgccggg gcaatacaca taaaaccaag
    57901 cggttttccg gcctgatgca tcgcttgtgc cagcgccttt aattcacggt caacggtgca
    57961 ttcgcgacca agactggcaa aattgcttaa attctttgcc gcgccaaacc cccccggcac
    58021 aatcaacgca tccagttcag cggcatcggc ctgggccaga ggacggattt caccgcgcgt
    58081 tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc atcgcttcgc cagttaaatg
    58141 attgataaca tcaacctgct gcttatccgg tgcaaagcag accgcctgcg caccgctgcg
    58201 tgaaatagct aacaacgtca acaccgcttc atgaatttca gaaccgtcat agacgccgca
    58261 tccgctcaga attacgccaa ttttcttcat tatgatcatc ctttcgcaat tgactgaaac
    58321 acattaattt ttttaataaa aatggtacgc atcacacatt taactgattc atgtaacaaa
    58381 tcatttaagt tttgctatct taactgcgtg cggcctgaaa aacagtgcag tgcccttgta
    58441 actcatcata ataatttacg gcgcagccaa gatttccctg gtgttggcgc agtattcgcg
    58501 caccccggtc tagccggggt cattttttag tggcttttgc cacccacgct ttcagcactt
    58561 ctacgtcgtg acgccactct tctttcatct cttcaatcca ttcaccgacg ttatcttccc
    58621 aggcgggaag gtcaggagac tgaatttgct gacccagctg ttgcagatgg cgcaacccca
    58681 ctgaccccgc cgcaccttta attttatgtc cttcctcaac aatgcctttt ttatcctgcg
    58741 ccgtcagatt cgactccagc acgttgacat agcctggcat catcttctca aacaccgcta
    58801 acccgtcggt gatcagcttc ggtcctacaa gttcgagata ctgttccagc atgggaatat
    58861 cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac cgtactctcc tcatcatcct
    58921 gggtatccca gaatttcttg atcatcgcgg ttagcgccgg aacagaaagc ggcttactca
    58981 gcacatcatc cattccagca ttgaggtact cttgtttgtc tttcagcacg ttagcggtta
    59041 aggccaccag cggcggtaaa tcctctcgcg gataacgctt cgtcagttca cgagagatat
    59101 ccagcccggt catatctggc aactgaatat ccagcaacac caggtcgtat tcgcccggtt
    59161 taaacatctc cagcgccgcc ttgccggtca tggcgacatc aacgctgtta cctaattttt
    59221 ccagcacaga acgcgcgaca atcacgttca gttcaatgtc ttccaccagc agcacattca
    59281 gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc gacctcttct gctaccgacg
    59341 gtgcgtggat cgtcaacgta aaggttgaac ctttaccctg ttcgctggta accgtaatat
    59401 cgccgcccat atttttcgcc agacgacgag aaacggccag accaataccg gtgccggtgg
    59461 caggtttacc gccatgactg tctttcacct ggtaatacat ggcgaagatt ttatccagct
    59521 catcctgcgg aatgccgata ccagaatctt ccacttcaaa atgcagcata tcgccttcat
    59581 cgtagcgcac gcgcacggta acctggcctt gctgggtgaa tttgacggcg ttactgatga
    59641 ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat gacctgatgc ggtaatggca
    59701 gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc ctgcaatgcg gagagatttt
    59761 ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt atcaagctgg accttgcgcc
    59821 gttccatctt atccatgtcg ataatatcgt taaagatatt ccccagcgtg acggccgaaa
    59881 catggatggt cttgagatat ttttcctgct cggcggtgag ttcggtatcc agcagaatgc
    59941 ggctcaggcc gacgataccg ttcagcggtg tacgcaattc gtggctgatg gtggaaataa
    60001 acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg ataccgctta cgctcggtaa
    60061 tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac gcggtcgtag tacggcactt
    60121 tacgaatttc aaagcaggct ttgcgtccgt cgggataatc cagccactgc tcataggtca
    60181 gtgacacatt atgacggaac actttttcat cggtttcaat gactttcgca gcggcttccg
    60241 gtgagtaaac atcagcaggt ttcaggtgaa ccagttgttt ttcgcttttt ccggtcagca
    60301 gctccatcgc gcggttacag ccggaaaact ctttatcttc gttacgataa aaaaccaggt
    60361 cgggtgaagc atcaaggaag gaacgtaaaa atgaggattg ctgctcgagc tgaatttgtg
    60421 tctcttcgcg ctctttgatt tcaattttca gttggccgaa ggtttcctgc agttctgctt
    60481 ccgctttttc acgaacggca atttcctgat ttagctgggc aatattatct tttaactgaa
    60541 cgttgaggct caaatcgcgc tcgcgcatct cctccagttt ttgcaccagc cgtgacagac
    60601 gttgtcgtga ctcctccagt tgctcgacga ccaccgatag aaagtagacc gcccacggcg
    60661 taatcagcaa accaaagaag atagaacgaa taacatcaat gctttcgacc tgaccatgca
    60721 gcaccatggt taccgccatt tgtaccacaa tggcaagaac gacgagggcc agcgccagca
    60781 acattgagaa gcgcaccaga cctaacttca tcatcaggtc aacataatac tgcgccagca
    60841 gacgaatttg cttcattagg gaattccttc acgacaacct gccccaataa tacccaattc
    60901 tgacagttac gttataaatt gtgtgagaaa tgcggaattc ccctcaacga cctactccgt
    60961 tggaggtagc cacggacgtc ccagcgccga tccctgtacg ccatgttcat tcagatagcg
    61021 atccagctcg accattcccg tccatcgatt ttcgcaccac agaggagcaa gcagcgtcgg
    61081 gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc ggtggcgtat ggcgaatcat
    61141 ttctccggca gtgagcgtgt aatcctccag ttcaataccg ttcaaacgcc ccgcttccca
    61201 ggctttcgcc ataatgctgc ctttcacaat atgcagcgga tgcagcttta tgccgtccac
    61261 gccggtttca accactcgct ccagcgtttg caaacattca gcctgccctt cgccaggcag
    61321 gccgacaatt aagtgagaac acaccttcag cccgcgctga cgtgccagct gggttgtacg
    61381 ctgataacag gcaaaatcat ggccgcgatt aatgcgatgc agggttttgt cgtgggcggt
    61441 ttgtagcccc agctccagcc acacttcgta gccctggtcc ttatattcgc aaagcagatc
    61501 cagcaccgca tccggcacgc agtccgggcg agtaccaaca cacaaaccga caatattggc
    61561 ctggctcacc gcctgctgat acatagaacg cagcacctga acttccgcaa aggtgctggt
    61621 atacgcctga aagtaggcca gatagcgttt agcgcggtta actaaattcg cctggtgcgc
    61681 cagttgctcg gcaatggaac gatgttgctg cgcttcatcg gcaaatgagg caacattaca
    61741 gaatgtgcag ccgccacgcc cgatggtacc gtcacggtta gggcagctaa aaccgccgtg
    61801 cagcgtcagc ttatgcacct tttgcccata acgacgggtg agatcaccac caaacatatt
    61861 gactaatttc tgtaactgca taatctgata gaccgcgcct tgaaaagagg ccaaagcctg
    61921 ccatttttag ctcaattcgg cgatgacttg gatcaatcgt cccggcctgc ttttatcaac
    61981 tgcataacca atcaaaatta ccgaaatttc atgcataatc acataaatca cttttgctta
    62041 tcttgtgtca gattttttta tctccttatg gattttaggc aaaacagtag catgaaacgt
    62101 cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg caggataagg
    62161 gttaacacgc ctttatgaca gtcaggaatt ggctgtttct ctaacgacgt cccttttagc
    62221 cttaattaga aaatccattt taatttcagt caattaataa agaattttgc gctaaagcac
    62281 atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata agttggaaat
    62341 ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta attgagatcc
    62401 cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc gaggcgcgcg
    62461 tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg gttcggaagt
    62521 ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat cccttgagag
    62581 ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc acaaggtagt
    62641 gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg
    62701 taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt ttcgcatcgt
    62761 tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc gtcgggatgc tcttcctgaa
    62821 taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga
    62881 aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat
    62941 cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg caggctggcg
    63001 tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc cgtattgaaa agcgtctcga
    63061 agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct ataaaggtct
    63121 gtgtatgccg gcggatctgc cgcgctttta tctagatctt gcggacctgc gtctggaatc
    63181 ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct ggccgctggc
    63241 gcaaccgttc cgctacctgg cgcataacgg tgaaatcaac accatcaccg gtaaccgcca
    63301 atgggcgcgt gcgcgtactt ataaattcca gacaccgctt atccctgacc tgcacgacgc
    63361 cgcaccgttc gtcaacgaaa tcggctctga ctccagttcg atggataaca tgctggaact
    63421 gctgctggca ggcgggatgg atatcatccg cgccatgcgt ctattagtac cacccgcctg
    63481 gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta actccatgca
    63541 tatggagccg tgggatggcc cggcgggcat cgtgatgtct gacggtcgtt ttgctgcctg
    63601 taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc atcaccaaag ataagctcat
    63661 cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg tcgaaaaagg
    63721 ccgcgtcggg ccaggcgaac tgatggtaat cgacactcgc agtgggcgta ttctgcactc
    63781 ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga tggagaaaaa
    63841 cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta gccgcgaact
    63901 ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg aagagctgga
    63961 ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga tgggcgatga
    64021 taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact tccgccagca
    64081 gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg ttatgtcgct
    64141 cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc aggcgcaccg
    64201 tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca cgacgatgaa
    64261 agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacca aaaccacgct
    64321 cgaagcgacg gtcaaagagc tgtgcgacaa agccgaaaaa atggtacata gcggcaccgt
    64381 gctgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc cggctccaat
    64441 ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat
    64501 catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc tgggtttcgg
    64561 cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg tagacaccca
    64621 cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca tcaacaaagg
    64681 cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc gctgctcgaa
    64741 actgtttgaa gcggtcggtc tgcacaatga tgtagtgggc ctgtgcttcc agggggcggt
    64801 cagccgcatt gggggggcga gctttgaaga cttccagcag gatctgctga atctctcgaa
    64861 acgagcctgg ctggcgcgca agcccatcag ccagggcggc ctgctgaaat acgtccacgg
    64921 cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg cggtacagag
    64981 cggcgagtat agcgactatc aggaatacgc gaagctggtt aatgagcgtc cggcaaccac
    65041 gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg ccgatgttga
    65101 accggcaagc gaactgttta aacgcttcga taccgcggcg atgtctatcg gcgcgttaag
    65161 cccggaagcc cacgaggcgc tggcggaagc gatgaacagc atcggcggaa actcgaactc
    65221 cggtgaaggt ggtgaagacc cggcgcgcta cggcaccaac aaagtgtcgc gcatcaagca
    65281 ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg gtcaatgccg acgtcattca
    65341 gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg gcgacaaagt
    65401 cactccttac attgccaaac tgcgctattc ggtgccgggc gtgacgctga tctccccgcc
    65461 gccgcaccac gatatctact ctatcgaaga cttagcgcag ctcattttcg acctcaagca
    65521 ggttaacccg aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg ttggcaccat
    65581 cgccaccggt gtggcgaaag cttatgccga tttaatcacc atcgcaggct atgacggcgg
    65641 caccggcgca agtccgcttt catcggtgaa atacgcaggc tgtccgtggg agctgggact
    65701 tgttgaaaca caacaggcgc tggttgccaa cggcttgcgt cataaaatcc gtttgcaggt
    65761 cgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg gcgcagaaag
    65821 cttcggcttc ggcactggcc caatggtcgc gctcggctgt aaatatctgc gtatttgcca
    65881 cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac aaactgcgta agaatcacta
    65941 tcacggcctg ccgttcaagg tgacgaatta ctttgagttt atcgcccgtg aaacccgcga
    66001 gctgatggca cagcttggcg taacacgtct ggtggatctg attggtcgca ccgacctgct
    66061 gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga agctgctgga
    66121 gaccgccgaa ccgcatccag gtaaggcact ctactgcacc gaaaccaacc cgccgtttga
    66181 taacggcctg ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg atgagcgcca
    66241 gagcaaaacc ttctggttcg atatccgcaa caccgaccgc tctgtcggcg cgtcgctttc
    66301 aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccga ttaaagcgta
    66361 cttcaacggc accgcaggcc agagcttcgg cgtgtggaac gcgggcggcg tggaactgta
    66421 cctgaccggt gatgccaacg actatgtcgg taaaggcatg gcgggcggct taatcgccat
    66481 tcgtcctccg gttggttccg ccttccgcag ccatgaagca agtattatcg gcaacacctg
    66541 cctgtatgga gcgacgggtg gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg
    66601 cgtgcgtaac tccggtgcaa tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata
    66661 tatgacgggc ggtatcgtct gtattctggg taaaaccggc gttaacttcg gtgcgggtat
    66721 gaccggcggc ttcgcttacg ttctcgatga aagcggcgat ttccgcaaac gcgttaaccc
    66781 ggaactggtg gaagtattaa gcgttgacga tttggcgatc catgaagagc atctgcgtgg
    66841 tcttatcacc gagcatgtgc agcataccgg ttcccagcgt ggtgaagaga ttctggcgaa
    66901 ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg aagtccagtg atgtcaaagc
    66961 actgctgggt catcgtagtc gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt
    67021 agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg atccgccaaa
    67081 gaaaccgctg aagatccgca aaattgagtt tgttgaaatt tacgagccgt tttccgaagg
    67141 ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc aacccatact gcgagtggaa
    67201 atgcccggta cacaactaca tcccgaactg gctgaagctc gccaacgagg ggcgtatttt
    67261 tgaagcggca gaactgtcgc accagaccaa cactctgccg gaagtatgcg gacgtgtctg
    67321 cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagtttg gcgcggtgac
    67381 catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct ggcgtccgga
    67441 tatgtccggc gtgaaacaga ccggtaaaaa agtggcgatt atcggcgcag gtccggcagg
    67501 tctggcgtgt gcagatgtcc tgacgcgcaa cggcgtacaa gcggtcgtct tcgaccgtca
    67561 cccggaaatt ggcggcttgc tgaccttcgg tattccggct ttcaaactgg aaaaagaggt
    67621 aatgacgcgc cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac tcaataccga
    67681 agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg tgttccttgg
    67741 cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg atggcgtgta
    67801 cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg gcgaaactcg
    67861 tgaagaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg gtggcgatac
    67921 cgcaatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta cctgtgccta
    67981 tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga
    68041 aggtgtagag ttcaaattca atgtccagcc actgggaatt gaagtgaacg gtaacggcaa
    68101 agtcagcggc gtaaaaatgg tgcgtactga aatgggcgaa ccggatgcca aaggccgtcg
    68161 ccgcgcggag atcgtggcag gttccgaaca tatcgttcca gcagatgcgg tgatcatggc
    68221 gtttggtttc cgtccacaca gcatggaatg gctggcaaaa cacagcgtcg agctggattc
    68281 gcaaggccgc atcatcgccc cggaaggcag cgacaacgct ttccagacca gcaacccgaa
    68341 aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg ctattgccga
    68401 aggtcgtaag gcggcagacg gcattatgaa ctggctggaa gtttaagctc tgtcccagcg
    68461 ccgcctcctc agaaaagagt cggcgctgaa attctccttt cgttctggaa agcgcctcgc
    68521 ataatctgaa gaaaaccaat gtaatacgca catctggatt gttaccggca gatgcccggc
    68581 gtatagctga ttccattatt caacactgcg ccatataaag ggaaagataa tggaatcgct
    68641 ctcagagaga acctcaacag gctaccagca aatccacgac ggtattattc acctggtcga
    68701 tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg atgaccgcga catactggga
    68761 aattggccga cgaattgtcg aatttgaaca aggtggcgag gccagggctg cgtatggtgc
    68821 gcagctaatc aagcgactat caaaggattt aagtctaagg tataagcgtg ggttctctgc
    68881 aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg aaattcgcca
    68941 gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg cagaattccc
    69001 atcggcaaag atttggcaga cgctgtctgc caaatctttc cccctgccct ggtccactta
    69061 cgtccgttta ctttccgtta aaaacgctga cacccgcagc ttttatgaaa aagagacgct
    69121 ccgctgtggc tggtctgttc gccagctaga acggcaaatt gcgacccaat tttatgagcg
    69181 aacactgctg tcacatgaca aatcagccat gctgcaacaa cacgctcctg ccgagacgca
    69241 tattcttccg caacaggcga tacgcgatcc ctttgtgctc gaatttctgg aattgaaaga
    69301 tgaatactca gaatccgatt ttgaggaggc gctgatcaac cacctgatgg atttcatgct
    69361 ggaacttggg gatgattttg cctttgttgg tcggcagcga aggttacgca ttgatgacaa
    69421 ctggtttcgt gtcgatctgc tgtttttcca ccgccgttta cgctgcctgc taatcgtcga
    69481 tctaaaagtg ggcaaattca gctatagcga tgccggacag atgaatatgt atctcaacta
    69541 cgccaaagag cactggacgc taccggatga aaatccgccc atcggtctgg ttctctgtgc
    69601 agagaaagga gtcggagaag cgcattatgc actggctggt ttgcctaaca ccgttctggc
    69661 gagtgaatat aagatgcaac tacctgatga aaaacgactc gcggatgaac tcgttcgaac
    69721 acaggcggtg ctagaagaag gctatagact ccgttaattt cagcggtttc gaattctgca
    69781 gacactgtct gcagaatctt taaaacgaac taagttttaa gccattaaat cagccttaac
    69841 cttcaccacc acctttttaa tctcaccagg ttcgccgaca acgcatcctg gtttatgcgg
    69901 ttctcccggc ataaacacgg cgaacattcc cggttttaag ttgatggttt gctcgttttc
    69961 aatggcgctg caaagctggt aatcatcttc atggtggaac tcttcacact ggcgcgcagt
    70021 gcctgccgtg ccaaacagaa tccgttcctc accttttaat aacagctgga tatcaatgta
    70081 ttgctcgtgc aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt
    70141 cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc
    70201 agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga
    70261 ctgtacttca cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa
    70321 cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc
    70381 gccagatacg tttccaccag cgccagatac ccttctgcca gaccaacgct gccaccgacc
    70441 accacgcact gacaatcagt tgtggcttta atatcagcga tcagccttgc cagcacatgt
    70501 gcggagcggt gaatcagctg ctgcgcctgc tcgtcgccct gcccggcgcg catgaaaata
    70561 gttttcgcat ccgcgccagc caactccccc tgcgctgccg ctgcaatacc gcgaccagag
    70621 gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca
    70681 agcgtatgcc cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc
    70741 actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatatcggt tatatcgcct
    70801 tccagcgcct gatactctgc ccatgctgcg gcctgggcgt cgttaatggc aatggtcggc
    70861 aaatcggtaa gttgccccag cgttttgact aacggaaagt gtagcaatcc accaagatta
    70921 tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc
    70981 cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct
    71041 ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca
    71101 atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt catagcaccg
    71161 cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac
    71221 cgaccgtcac cgcccacgcg ccgtggcgca tcgcatcagc cgcctgagca ggcgtgttgt
    71281 aacgcccttc ggcaatcacc cgacatccgg cgtcgctcaa ccttttcacc agcgtcagat
    71341 ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg
    71401 ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta
    71461 aaccgtgatg atgaatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg
    71521 tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca tcttcaatat
    71581 aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca
    71641 cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga atggcaaccg
    71701 cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta tcgagcgggc
    71761 tgtccggaac cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca
    71821 gttgtgcaag taacgacata catcttccct tagcgaaagg cccgatacag agaccgggca
    71881 acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaacg gcacggcacc
    71941 gctaaatggt ttgccgtcga tagcgtcatg agtacgcaac gcttccgggc gcagccaacg
    72001 ctgaacacga gaaggcatat ccagaccaat cagcaggatc accacaaatg tcagactgaa
    72061 cgagagcgat gccagtgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat
    72121 tggggccagt gcgccaccca atgcgccaac gttataggta aagcccaggc ccgctgcacg
    72181 ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc
    72241 aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcac cgccaatagc
    72301 aaataccgga ataatcagca gctgcgaggc caacagacta caaacgtacg ctttgcgggt
    72361 tcccagccag tcaccaagga agccaccgac gcagcacccc accgccgcgc caaagccact
    72421 aaagaacagc acattggcga cagtatgagg gttataagcc agatcggttt tcagatacgt
    72481 tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag
    72541 cattacgcca gttggccagc gtttgcccgt actctgcacc ataaagctga taaagattgc
    72601 ggtgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac cggcgaagca
    72661 gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc
    72721 acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca
    72781 gtcttctgct tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc
    72841 gataaagaac agcgcacgcc agccccagac cggaaccacc aggctgtaga cctgagcggc
    72901 aacgacggcc cctacagaga agcctgaaat cagaaagcca ctggctttgt tacgcagatg
    72961 ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat
    73021 gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcatgccag
    73081 cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc
    73141 acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag
    73201 acttgccgcc tgcaccgtcg tcagcccgaa ttcaccttgt acttcggtga gtaccagggc
    73261 gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcccg
    73321 ccattgtgca cgattgagat ggcgatacca cgggatattc tgggttgtag tactcattgt
    73381 gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg
    73441 cgtttatttc ccctcacccg gtaggggcga gcgaggggaa acaactcacc cgcgctcttg
    73501 catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggaccaaa
    73561 cggtttgcgg cacagcggca cagaaacgac atccatataa tgcagaacag ttttcaggcc
    73621 gcggaataca cccgttttga tcagtaaatc aatgactttg ttgcattcag tttgcagttt
    73681 ctgcgcggtc tggatatcgc cttctttcag cgccttaacg atcccctgat agcgccagcc
    73741 catgatgttg taggtactac cgataccacc atcagcgccc gccagcaggc cagaggcgaa
    73801 gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga tctgctccat
    73861 ctgatagaga tcgccagagg tctgtttcag cgcacctacg ccaggcaatg taacaagtgt
    73921 gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt acaccaccat
    73981 cggcaaacca tccgccgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct
    74041 gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga
    74101 tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat
    74161 cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg aaaggctttg
    74221 tacaaaggct tcgccggtcg aaccacccac gtataaaccg tcaatgccct gctgaatatt
    74281 gaactgaacc aggcggcgca gactcgcttt atccagtgct tgttgttgat caaaaggagt
    74341 caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg aagtgatgct
    74401 tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc aacaccccat
    74461 acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt ttgttggtgg
    74521 tctgaccgaa ggcgtgccag gtagcagaga cgctgttgag atgcgattgc aacgcacgat
    74581 cggcttcgtc aggatcgtgg cggcggatcg cgtcaacgat cgcaatatgc tgttgatagc
    74641 taacgttgtt atgctcgtgc agagcctgat cagccacggt tggacgtgcg gcgatcagcc
    74701 agtcgagcag ggcaacgtgg atcgccatga agattgggtt accggggatc tccgccagca
    74761 cgcggtggaa atcaacgtct gagcgaatga atgccgcgtt gttatccagc gactgactgt
    74821 tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcgtgt tcagccgcat
    74881 agcgcaccag gctggattca aagaacagac gtaattgttc gaaatgggca atcccaccag
    74941 gatgagaaag gaaatctttc gccatgccgg aaagctcgcc gatgatggtg tccgcagaag
    75001 gacgtgagac gcgggcacgt tcgccgttgt ttatttgcac cagacctttg cgttttaacg
    75061 ctgccagcgc ttcacgcacc gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt
    75121 cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt tccagctctt
    75181 cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag ttgcgaccaa
    75241 ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc attctgtaag
    75301 gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta ggggaaaatg
    75361 gcagggtttt ctctttgtgc ctcatcatta ccataattaa cggaataatt aactattgcg
    75421 aaaaattaat gtaacgcag
//
LOCUS       DAZGXS010000008        69954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGXS010000008 DAZGXS010000000
VERSION     DAZGXS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            159..1223
                     /gene="ulaG"
                     /locus_tag="ACHKG5_000630"
     CDS             159..1223
                     /gene="ulaG"
                     /locus_tag="ACHKG5_000630"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0866800.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1331..2086
                     /gene="ulaR"
                     /locus_tag="ACHKG5_000631"
     CDS             1331..2086
                     /gene="ulaR"
                     /locus_tag="ACHKG5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0866801.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2083..2832)
                     /gene="yjfP"
                     /locus_tag="ACHKG5_000632"
     CDS             complement(2083..2832)
                     /gene="yjfP"
                     /locus_tag="ACHKG5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0866802.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3014..3343
                     /gene="bsmA"
                     /locus_tag="ACHKG5_000633"
     CDS             3014..3343
                     /gene="bsmA"
                     /locus_tag="ACHKG5_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0866803.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3492..3767
                     /gene="yjfN"
                     /locus_tag="ACHKG5_000634"
     CDS             3492..3767
                     /gene="yjfN"
                     /locus_tag="ACHKG5_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0866804.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3884..5509)
                     /gene="aidB"
                     /locus_tag="ACHKG5_000635"
     CDS             complement(3884..5509)
                     /gene="aidB"
                     /locus_tag="ACHKG5_000635"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0866805.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5593..6756)
                     /gene="yjfC"
                     /locus_tag="ACHKG5_000636"
     CDS             complement(5593..6756)
                     /gene="yjfC"
                     /locus_tag="ACHKG5_000636"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0866806.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6759..7397)
                     /gene="yjfM"
                     /locus_tag="ACHKG5_000637"
     CDS             complement(6759..7397)
                     /gene="yjfM"
                     /locus_tag="ACHKG5_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0866807.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7407..7805)
                     /gene="yjfL"
                     /locus_tag="ACHKG5_000638"
     CDS             complement(7407..7805)
                     /gene="yjfL"
                     /locus_tag="ACHKG5_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0866808.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7823..8482)
                     /gene="yjfK"
                     /locus_tag="ACHKG5_000639"
     CDS             complement(7823..8482)
                     /gene="yjfK"
                     /locus_tag="ACHKG5_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0866809.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8533..9231)
                     /gene="yjfJ"
                     /locus_tag="ACHKG5_000640"
     CDS             complement(8533..9231)
                     /gene="yjfJ"
                     /locus_tag="ACHKG5_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0866810.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9250..9651)
                     /gene="yjfI"
                     /locus_tag="ACHKG5_000641"
     CDS             complement(9250..9651)
                     /gene="yjfI"
                     /locus_tag="ACHKG5_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP0866811.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9778..10509)
                     /gene="rlmB"
                     /locus_tag="ACHKG5_000642"
     CDS             complement(9778..10509)
                     /gene="rlmB"
                     /locus_tag="ACHKG5_000642"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0866812.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10690..13131)
                     /gene="rnr"
                     /locus_tag="ACHKG5_000643"
     CDS             complement(10690..13131)
                     /gene="rnr"
                     /locus_tag="ACHKG5_000643"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0866813.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13170..13595)
                     /gene="nsrR"
                     /locus_tag="ACHKG5_000644"
     CDS             complement(13170..13595)
                     /gene="nsrR"
                     /locus_tag="ACHKG5_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0866814.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13800..15098)
                     /gene="purA"
                     /locus_tag="ACHKG5_000645"
     CDS             complement(13800..15098)
                     /gene="purA"
                     /locus_tag="ACHKG5_000645"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0866815.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15202..15399)
                     /gene="yjeT"
                     /locus_tag="ACHKG5_000646"
     CDS             complement(15202..15399)
                     /gene="yjeT"
                     /locus_tag="ACHKG5_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0866816.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15481..16485)
                     /gene="hflC"
                     /locus_tag="ACHKG5_000647"
     CDS             complement(15481..16485)
                     /gene="hflC"
                     /locus_tag="ACHKG5_000647"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0866817.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16488..17747)
                     /gene="hflK"
                     /locus_tag="ACHKG5_000648"
     CDS             complement(16488..17747)
                     /gene="hflK"
                     /locus_tag="ACHKG5_000648"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0866818.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGSDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17833..19113)
                     /gene="hflX"
                     /locus_tag="ACHKG5_000649"
     CDS             complement(17833..19113)
                     /gene="hflX"
                     /locus_tag="ACHKG5_000649"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0866819.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19190..19498)
                     /gene="hfq"
                     /locus_tag="ACHKG5_000650"
     CDS             complement(19190..19498)
                     /gene="hfq"
                     /locus_tag="ACHKG5_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0866820.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19584..20534)
                     /gene="miaA"
                     /locus_tag="ACHKG5_000651"
     CDS             complement(19584..20534)
                     /gene="miaA"
                     /locus_tag="ACHKG5_000651"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0866821.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20527..22374)
                     /gene="mutL"
                     /locus_tag="ACHKG5_000652"
     CDS             complement(20527..22374)
                     /gene="mutL"
                     /locus_tag="ACHKG5_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0866822.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22384..23721)
                     /gene="amiB"
                     /locus_tag="ACHKG5_000653"
     CDS             complement(22384..23721)
                     /gene="amiB"
                     /locus_tag="ACHKG5_000653"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0866823.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23740..24201)
                     /gene="tsaE"
                     /locus_tag="ACHKG5_000654"
     CDS             complement(23740..24201)
                     /gene="tsaE"
                     /locus_tag="ACHKG5_000654"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0866824.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24173..25720)
                     /gene="nnr"
                     /locus_tag="ACHKG5_000655"
     CDS             complement(24173..25720)
                     /gene="nnr"
                     /locus_tag="ACHKG5_000655"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0866825.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25719..26858
                     /gene="queG"
                     /locus_tag="ACHKG5_000656"
     CDS             25719..26858
                     /gene="queG"
                     /locus_tag="ACHKG5_000656"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0866826.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26841..26894)
                     /locus_tag="ACHKG5_000657"
     CDS             complement(26841..26894)
                     /locus_tag="ACHKG5_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866827.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(27128..27203)
                     /locus_tag="ACHKG5_000658"
     tRNA            complement(27128..27203)
                     /locus_tag="ACHKG5_000658"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27168..27170),aa:Gly,seq:gcc)
     gene            complement(27239..27314)
                     /locus_tag="ACHKG5_000659"
     tRNA            complement(27239..27314)
                     /locus_tag="ACHKG5_000659"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27279..27281),aa:Gly,seq:gcc)
     gene            complement(27351..27426)
                     /locus_tag="ACHKG5_000660"
     tRNA            complement(27351..27426)
                     /locus_tag="ACHKG5_000660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27391..27393),aa:Gly,seq:gcc)
     gene            complement(27637..28182)
                     /gene="orn"
                     /locus_tag="ACHKG5_000661"
     CDS             complement(27637..28182)
                     /gene="orn"
                     /locus_tag="ACHKG5_000661"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0866828.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            28277..29329
                     /gene="rsgA"
                     /locus_tag="ACHKG5_000662"
     CDS             28277..29329
                     /gene="rsgA"
                     /locus_tag="ACHKG5_000662"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0866829.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            29426..30394
                     /gene="asd"
                     /locus_tag="ACHKG5_000663"
     CDS             29426..30394
                     /gene="asd"
                     /locus_tag="ACHKG5_000663"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0866830.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            30416..33739
                     /gene="mscM"
                     /locus_tag="ACHKG5_000664"
     CDS             30416..33739
                     /gene="mscM"
                     /locus_tag="ACHKG5_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0866831.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTITWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33890..35392)
                     /gene="yjeM"
                     /locus_tag="ACHKG5_000665"
     CDS             complement(33890..35392)
                     /gene="yjeM"
                     /locus_tag="ACHKG5_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0866832.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(35611..36588)
                     /gene="epmA"
                     /locus_tag="ACHKG5_000666"
     CDS             complement(35611..36588)
                     /gene="epmA"
                     /locus_tag="ACHKG5_000666"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0866833.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            36913..38721
                     /gene="frdA"
                     /locus_tag="ACHKG5_000667"
     CDS             36913..38721
                     /gene="frdA"
                     /locus_tag="ACHKG5_000667"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0866834.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            38714..39448
                     /gene="frdB"
                     /locus_tag="ACHKG5_000668"
     CDS             38714..39448
                     /gene="frdB"
                     /locus_tag="ACHKG5_000668"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0866835.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            39459..39854
                     /gene="frdC"
                     /locus_tag="ACHKG5_000669"
     CDS             39459..39854
                     /gene="frdC"
                     /locus_tag="ACHKG5_000669"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0866836.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            39865..40224
                     /gene="frdD"
                     /locus_tag="ACHKG5_000670"
     CDS             39865..40224
                     /gene="frdD"
                     /locus_tag="ACHKG5_000670"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0866837.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            40287..41420
                     /gene="ampC"
                     /locus_tag="ACHKG5_000671"
     CDS             40287..41420
                     /gene="ampC"
                     /locus_tag="ACHKG5_000671"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0866838.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            41508..42041
                     /gene="blc"
                     /locus_tag="ACHKG5_000672"
     CDS             41508..42041
                     /gene="blc"
                     /locus_tag="ACHKG5_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0866839.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(42038..42355)
                     /gene="sugE"
                     /locus_tag="ACHKG5_000673"
     CDS             complement(42038..42355)
                     /gene="sugE"
                     /locus_tag="ACHKG5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0866840.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(42531..42677)
                     /gene="ecnB"
                     /locus_tag="ACHKG5_000674"
     CDS             complement(42531..42677)
                     /gene="ecnB"
                     /locus_tag="ACHKG5_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0866841.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(42788..42913)
                     /gene="ecnA"
                     /locus_tag="ACHKG5_000675"
     CDS             complement(42788..42913)
                     /gene="ecnA"
                     /locus_tag="ACHKG5_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0866842.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(42965..43531)
                     /gene="efp"
                     /locus_tag="ACHKG5_000676"
     CDS             complement(42965..43531)
                     /gene="efp"
                     /locus_tag="ACHKG5_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0866843.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            43573..44601
                     /gene="epmB"
                     /locus_tag="ACHKG5_000677"
     CDS             43573..44601
                     /gene="epmB"
                     /locus_tag="ACHKG5_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0866844.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            44836..45705
                     /gene="yjeJ"
                     /locus_tag="ACHKG5_000678"
     CDS             44836..45705
                     /gene="yjeJ"
                     /locus_tag="ACHKG5_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0866845.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(45898..46251)
                     /gene="yjeI"
                     /locus_tag="ACHKG5_000679"
     CDS             complement(45898..46251)
                     /gene="yjeI"
                     /locus_tag="ACHKG5_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0866846.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(46389..48044)
                     /gene="groL"
                     /locus_tag="ACHKG5_000680"
     CDS             complement(46389..48044)
                     /gene="groL"
                     /locus_tag="ACHKG5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0866847.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            complement(48088..48381)
                     /gene="groS"
                     /locus_tag="ACHKG5_000681"
     CDS             complement(48088..48381)
                     /gene="groS"
                     /locus_tag="ACHKG5_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0866848.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            48657..49913
                     /gene="yjeH"
                     /locus_tag="ACHKG5_000682"
     CDS             48657..49913
                     /gene="yjeH"
                     /locus_tag="ACHKG5_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0866849.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(49929..50405)
                     /gene="fxsA"
                     /locus_tag="ACHKG5_000683"
     CDS             complement(49929..50405)
                     /gene="fxsA"
                     /locus_tag="ACHKG5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0866850.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            50742..52178
                     /gene="aspA"
                     /locus_tag="ACHKG5_000684"
     CDS             50742..52178
                     /gene="aspA"
                     /locus_tag="ACHKG5_000684"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0866851.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            52296..53597
                     /gene="dcuA"
                     /locus_tag="ACHKG5_000685"
     CDS             52296..53597
                     /gene="dcuA"
                     /locus_tag="ACHKG5_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0866852.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            53713..54051
                     /gene="cutA"
                     /locus_tag="ACHKG5_000686"
     CDS             53713..54051
                     /gene="cutA"
                     /locus_tag="ACHKG5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0866853.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            54027..55724
                     /gene="dsbD"
                     /locus_tag="ACHKG5_000687"
     CDS             54027..55724
                     /gene="dsbD"
                     /locus_tag="ACHKG5_000687"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0866854.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            55761..56336
                     /gene="yjdC"
                     /locus_tag="ACHKG5_000688"
     CDS             55761..56336
                     /gene="yjdC"
                     /locus_tag="ACHKG5_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0866855.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            56443..56518
                     /locus_tag="ACHKG5_000689"
     tRNA            56443..56518
                     /locus_tag="ACHKG5_000689"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56476..56478,aa:Phe,seq:gaa)
     gene            56716..56875
                     /locus_tag="ACHKG5_000690"
                     /pseudo
     CDS             56716..56875
                     /locus_tag="ACHKG5_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            57135..58673
                     /gene="cadC"
                     /locus_tag="ACHKG5_000691"
     CDS             57135..58673
                     /gene="cadC"
                     /locus_tag="ACHKG5_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP0866856.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            59039..60373
                     /gene="cadB"
                     /locus_tag="ACHKG5_000692"
     CDS             59039..60373
                     /gene="cadB"
                     /locus_tag="ACHKG5_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP0866857.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            60453..62600
                     /gene="cadA"
                     /locus_tag="ACHKG5_000693"
     CDS             60453..62600
                     /gene="cadA"
                     /locus_tag="ACHKG5_000693"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP0866858.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            62659..64116
                     /gene="dtpC"
                     /locus_tag="ACHKG5_000694"
     CDS             62659..64116
                     /gene="dtpC"
                     /locus_tag="ACHKG5_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP0866859.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            64353..65870
                     /gene="lysS"
                     /locus_tag="ACHKG5_000695"
     CDS             64353..65870
                     /gene="lysS"
                     /locus_tag="ACHKG5_000695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0866860.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(65989..66162)
                     /gene="ghoT"
                     /locus_tag="ACHKG5_000696"
     CDS             complement(65989..66162)
                     /gene="ghoT"
                     /locus_tag="ACHKG5_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0866861.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(66190..66486)
                     /gene="ghoS"
                     /locus_tag="ACHKG5_000697"
     CDS             complement(66190..66486)
                     /gene="ghoS"
                     /locus_tag="ACHKG5_000697"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0866862.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            complement(66713..66985)
                     /gene="yjdJ"
                     /locus_tag="ACHKG5_000698"
     CDS             complement(66713..66985)
                     /gene="yjdJ"
                     /locus_tag="ACHKG5_000698"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0866863.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(66997..67227)
                     /gene="yjdI"
                     /locus_tag="ACHKG5_000699"
     CDS             complement(66997..67227)
                     /gene="yjdI"
                     /locus_tag="ACHKG5_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0866864.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            67408..69039
                     /gene="dcuS"
                     /locus_tag="ACHKG5_000700"
     CDS             67408..69039
                     /gene="dcuS"
                     /locus_tag="ACHKG5_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0866865.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            69036..69755
                     /gene="dcuR"
                     /locus_tag="ACHKG5_000701"
     CDS             69036..69755
                     /gene="dcuR"
                     /locus_tag="ACHKG5_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0866866.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT        17202 a        18314 c        17202 g        17236 t
ORIGIN      
        1 tatacattcc attaaatctt tccatactca aaatcaacaa caacttccct ggcaaaaagt
       61 caaaaaattg tcattgcctt ccagaaaaaa tagtgtcagg attaatcaaa accaatcaca
      121 tattgattcg aattgaacta tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca
      181 cccgtgaatc ctggatcctg agcactttcc cggagtgggg tagctggttg aatgaagaaa
      241 ttgaacaaga acaggtcgct cctggcacat ttgcgatgtg gtggcttggc tgcaccggga
      301 tctggttgaa atcggaaggt ggcaccaacg tttgcgttga tttctggtgc ggcactggca
      361 aacaaagtca cggtaacccg ttaatgaaac agggtcacca gatgcagcgc atggctggcg
      421 tgaaaaaact gcagccaaac ctgcgtacca ccccgtttgt tcttgatccg tttgcgattc
      481 gccagatcga cgcggtactg gcgactcacg atcacaacga tcatatcgac gttaacgtcg
      541 ctgctgccgt gatgcagaac tgtgctgatg acgtaccgtt tatcggaccg aaaacctgtg
      601 tggatttgtg gattggctgg ggcgtaccga aagagcgttg catcgtggtc aaaccgggcg
      661 atgtagtaaa agtgaaagac attgaaattc atgcgcttga tgctttcgac cgtactgcac
      721 tgatcaccct gcctgccgat caaaaagcgg ctggcgtact gccagatggc atggacgatc
      781 gcgcggtgaa ctacctgttc aaaacgcctg gcggctccct gtatcacagc ggcgactccc
      841 actactctaa ctattatgcg aagcacggta acgaacatca gatcgacgtg gcgttaggat
      901 cgtacggcga aaacccgcgc ggtatcaccg acaagatgac cagcgccgat atgctgcgta
      961 tgggtgaagc gctgaatgcg aaagtggtta tcccgttcca ccacgatatc tggtcaaact
     1021 tccaggccga tccgcaagag atccgcgtgc tgtgggagat gaaaaaagat cgtctgaagt
     1081 atggcttcaa gccgtttatc tggcaggtgg gcggcaaatt tacctggccg ctggataaag
     1141 acaacttcga gtaccactat ccgcgcggtt tcgatgattg cttcactatt gaaccggatc
     1201 tgccgttcaa gtcattcctg taatctgtcg tttataccgg atagcgtgga gaagttcgcg
     1261 ctatccggcg tagtatttcc tggctatttc aaatatcatc taaaaaaatc aaattttatc
     1321 ggaatagctc atgactgaag cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt
     1381 gggctttgtg accgttgaga aagtcgttga gcgtctggga atttcgcctg ccactgcgcg
     1441 acgcgatatc aataaacttg acgaaagcgg caaactgaaa aaagtgcgca atggtgcaga
     1501 agctattacc caacagcgcc cgcgctggac gccgatgaat ctgcatcagg cgcagaatca
     1561 cgatgaaaaa gtacgtatcg ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt
     1621 agtcatcaac tgcggctcca ccgcgtttct gcttgggcgg gaaatgtgtg gcaagccagt
     1681 gcaaatcatc actaattatc taccgctggc aaattacctg atcgatcaag aacatgacag
     1741 cgtgatcatt atgggcggac agtacaacaa aagtcagtcc atcactttaa gcccgcaggg
     1801 cagcgaaaac agtctctatg ccgggcactg gatgtttacc agcggaaaag ggctgaccgc
     1861 agaagggttg tataaaaccg atatgctgac agcaatggca gagcagaaga tgctgagcgt
     1921 ggtagggaaa ctggtggtac tggttgatag cagtaagatt ggcgaacgcg cgggaatgct
     1981 ttttagccgt gccgatcaaa tcgatatgct tatcaccggc aaaaatgcta acccggaaat
     2041 cctgcaacaa ctggaagcgc aaggggtcag cattctgcgt gtttaaagat gctggcggaa
     2101 aaatgtcacg gcagcatcta acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca
     2161 tgaacaggtt agcagtttat cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga
     2221 ttcgtcggca ggcacaacgt catcgtcgag gccataccac agcagtagag gtctgtcact
     2281 aagttgttcc aggtggtttg tcgcttccca ctctgccagt ggcgcgacaa tgttattgaa
     2341 ttcattctgc tgtgctaccg tttcaggtat cagcggtgga aacagtgaac gggcgagtga
     2401 tgtaaaatag cccgatccca tcatgctggc ggtacatctc accgtggggt ggcgagcggt
     2461 aatccccagt gccgtcatcg cgcccatcga cgcaccaccg actgccagac ggtcatcaag
     2521 cagccacttt tcttcggcta ttgccgcacg taaagtagtg aattcctgca tactttgtag
     2581 caagatttgc cagaattgat ttaaccgccg cgctgcgtca ccactaaaac ggctaccgtg
     2641 atcgggcgca tccggcatga tcacccgcaa accagcctgc gccagcgcaa cggcaaaata
     2701 gctatacacc agactggacg aagtaaagcc gtgataaaaa atcacgcacg gtaacggggt
     2761 atctttttgt ccgacaggat aagcatgaag aacgggaata tctgccagct cgcgtgattc
     2821 tatttcaatc atctgtctct cctttttgtc tggtatgaca tgaagaaatc cttagcacaa
     2881 gtttctgctg gcggtattag ttcataatgt tgagatgtgg gttacgcttt cgtcacgttt
     2941 tcattcgaaa atcgcgtacc aggtaacaat tcgggaacat tcccccaaag ttaaattaat
     3001 aaggcactac actatggtta gcaggaaacg taatagcgtt atttaccggt ttgccagttt
     3061 attattggtg ttgatgttaa gtgcctgtag cgcactgcaa ggtacgccac agccagcacc
     3121 gccagtaacg gaccatccgc aagagattcg ccgcgaccag acgcaagggt tacagcgaat
     3181 aggtagcgta agcacaatgg ttcggggttc tccggatgac gcattagcag aaatcagagc
     3241 aaaagctgtc gctgcaaaag ctgattatta cgttgtcgtc atggtggacg aaaccatcgt
     3301 gacaggacag tggtattcac aagccatttt gtaccgtaaa taaatcagac aacttacata
     3361 gtcagcttta cattgctttg cgtccctatg cgtttacctg tgcacaataa attaccggct
     3421 ggaagcctgg gcgtttatta ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa
     3481 tggagctgac gatgaaacaa ttacttgcct caccctcgct gcaattagtg acttatcctg
     3541 cgagcgccac ggcacagtct gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg
     3601 aaataggtca gatctcagtt agcaatattt caggggatcc gcaggacgtg gaacgtatcg
     3661 tggcgttaaa ggccgatgaa caaggtgcgt catggtaccg cattattacc atgtacgaag
     3721 accagcagcc tgataactgg cgcgtacagg cgatactgta tgcatgagtg tcatctacag
     3781 caaatgaaca atgccggatg cggcgcgagc gccttgtccg gcctacaatt ttcacatatt
     3841 tcatttagtt aatcgaaacc agcgtcgcat cagtcgtata cgcttacaca cacactcccc
     3901 ccgtcgcccg cagcaataaa tcattctgga tctgctctga caaccgtacg ccgccgcgcg
     3961 tatctaacat cacctgacac cacgcctgcg ccattggcgg agaagcatat ttcaacattt
     4021 gcgcaccgca gcccagcagg aatagctgat gagtaatctc tcgccccagt tcttcagctg
     4081 gcttacgcag ctgctgctgt aaacgacgaa ccgcgcaatc aaaatagcga tcctgccctt
     4141 tcacttctac aaatgcttcc gacaataagt cgtatacgcc cgcttgctta ttgagaacgc
     4201 gcagcacatc caggcacata atattgccgg aaccttccca aatactgttt accggcatct
     4261 cccggtaaag ccgcggcagc tcgctctcct cgcaataacc aatgccgccc agcacctcca
     4321 tcgcttcggc cacaaacggc atacctcgtt tgcagatcac aaattttgcc gcaggcgtaa
     4381 ataaacgcgc ccacagagct tctttggcat cggcgcgccg gtcccacgct cgcgcaagac
     4441 gaaacagcaa cgccgtttgc ccttcaagct gaagtgccat gcgacttaag acatgacgca
     4501 taaggggctg ttggatcaat ggattaccaa aaacatggcg ttgatgcgca tgataaatcg
     4561 ccagcgagaa cgcacggcgc atcatggcat ggctacccag ggcgcaatca aaacgcgtca
     4621 tcccccccat tttcaggatc agacgaattc cttccccttc ctgccccaat aaccagccaa
     4681 tggcatcctg aaactccact tcacaactgg cgttagagcg attacccagc ttatctttca
     4741 gccgctcgag gcgaatcgcg ttgcgttgcc cgtcaggcaa aaagcgcggc acaaaaaagc
     4801 aggacaaacc gcccgcggtc tgcgccagca ccagatgcgc atcgctttgc ggaactgaga
     4861 aaaaccattt atgccccacc agccgataag agccatcctc cagacgctct gcgcgggtgg
     4921 tgttgctcat gacatcggaa ccgccctgct tttccgtcat tcccatgcca atcaacaaac
     4981 cgcgtttttg cccacctggc aataagtgag aatcgtagcg atcgctcaac agcggtgtgg
     5041 tccagtcctg aaacggcgcg ggtaacatct gtaacaacaa tggcgtggcg gcaaaggtca
     5101 tggttatcgg acataacgac cccgcctcaa cctgtgcgtg caacataaaa cgcgccgcgc
     5161 gcgccacaaa tgcgccggag cgagcgtctt cttcccaggc aagattgtgc acccgattgg
     5221 tacatagtgc ctgcatcagc aggtgccagg cggggtgaaa acgcacatcg tccagacggc
     5281 gtccttgcgc atcgtagcgc aacagttcag gcggattcac attcgccagc cgccccagtt
     5341 caagggattc agccgttcct aactgctgac caatactggc tagaaaatcg ctatcccagc
     5401 cagcaccttc acgcgttacc gcttcgcaaa gcgcgccatc agacaggtat aagttgctgt
     5461 tatttaatgg tataggttga ttaaaaacgg tgtgagtttg ccagtgcact gtgtctccct
     5521 ccatcaatgg cagtcaccat tagtatggtc actgccatgg attcatgaca gaaagagatt
     5581 gctaaaacat tcttatccag caatgtaatg cggaacgaaa cgtgaggtgt ctttggtgat
     5641 cagtgtgtta tcttcacgga tccccattcc gcacgcttca tcatcgacaa tccagctacc
     5701 gatgagtgtg tagctatcgc caaaccgcgg cagaggttga aacgcctggt agatcatcgg
     5761 ttcatcggcg taatcaccat cagcgtggtc aacgacgtta ttctggccgt cgaaaatggt
     5821 gacatttccg ccttcgcgcg agtagatcgg tttacgcaca tagctttcgc cagcggcaat
     5881 ctgcggtttt tcaccctcaa accaggatgc aagaaggtta ggatgaccag ggaagaagcg
     5941 ccaaagcagc ggcattagcc ctttattact caagatactt ttccataacg gctccaccca
     6001 ttgctcgcga cgcttgcgca gcagcggacc gttatcgtca cgcatcatcc actccagcgg
     6061 atacagctta aatgcacgct ggatgacatt atcatcaaga tcggtcagta cgccgccgac
     6121 gcccaaaccg agatcttcaa tgtagataaa ccgcgactcc tgccctgcct gctgggcgca
     6181 gtcctgcaag tacagcacgg tactcctgtc ttcatcggtg tcctgacagc agcaaaaata
     6241 aaacggttcc cgactgtaaa gctcgctaaa gcgtgaaatc aggcgttcct gaatagcatt
     6301 gtactgatcg gcatcacgcg gaataatgcc gctgcgccgg gcatcctcca gccacagcca
     6361 ctggaaataa gccgactcgt acaatgaagt tggcgtatcg gcgttgtact ccagcagctt
     6421 caccggcgca ttgccacacc aggcaaaatc catccgtcca tacagcgaag gatcgcgggc
     6481 gcgccagctt tcagcgatca catcccagta caacggcggg atcgccagtt gcgtcaggat
     6541 ctcttcatct tttaccgcgc gatccaccac ctcaaggcac atctgatgca gttccgcagt
     6601 cggtttttcg atctgctctt caatctggcg cagagtaaaa cggtaagccc gactctcatc
     6661 ccaatagatt tcattgtcga tgatatgaaa gtcgaaaccg ttgtcagcgg cgatctggtc
     6721 cagatcccgt cgcacaggaa cgttgtgtct cagcatgatt agcctcccca atgcccacgg
     6781 gcgctggaag aacgaccata gccgccgcga gaaacggttg acgctttttt ggtggtaaag
     6841 ccgcctgaag agtaagactc ttttttgcca gaaccggagc gccaggagta atcaccggaa
     6901 gtgttgcgcc agacggggcg ggaagcaaat gaggatccgc cgctgttata gacaaacggc
     6961 tcatcgcgat ctttacgaat aacccggctt aacaaaaatc cagaaacgac cgggatccaa
     7021 ctctgttcaa cattgtcgta gtagcaattt tcgtacttag actgacagtt ctgctgagtc
     7081 atattcttcg gaacatcggc ataaaatgcc gctttggcgt tgttccagcc acgcgcgcaa
     7141 atgtcggcat tattaccgtc atcaatacaa tcctgcactg tcgaataaaa cgttccgtcg
     7201 ccgtcgttat cgacatcgcc actatcgcca caacctttca atacaaaaaa agcggcaccg
     7261 cccattagcg ccaacgttaa gtatttttgt gcgtagcgat tgcgacgcgg ttcaaaagga
     7321 ttattcggtc taccaatgcg actgatcgct ccgtgaccaa ttttactgtt atttcttgat
     7381 ttgcgttttc tggccatgat cgtccattac catgtcatac aagctgcgtt aaaaataccg
     7441 ccagccagcg cggcggttcc cataaacatt cctgctgcgg tattgtgatt aataattttt
     7501 tcgcttaatg cgggcatata aagtctgaca ccagcaaaaa cgagtaactg aatcaccagt
     7561 gcaatcccgc cccaggcgaa atagtctgga atactcaccg cattgattgc cgcactggat
     7621 aaggggatca cgtaacccaa caatgtaccg ctgaacgcca gtgacgctgc ggtattattg
     7681 tttttgatta attgccattc attgtgcggt gtaattttgg agtagataaa cagaaaaata
     7741 atcaccatag ccacgccaat aaaaaaatag gcactaaagg caagaagtga atccagtata
     7801 tgcattgtaa acgtccttgt aattaaccaa taatatgaag tgatgtcagc gggatatcga
     7861 cacccaacgc gcgcgaaaat aaccactctg gctcgccgag atcgttaaaa gattcctcac
     7921 catttagcag caaatattcg taagtatctt cggtcacttg ccgctggtag cccatggtaa
     7981 aattatggac ttcccattta gcatgatttt gattttctac tttttccagc atgtagacgg
     8041 gttcgatatt tcccggttct tcgctgttaa aaaatcgctg ccagcgtttt tcctgccagt
     8101 ttaaggtcat tgcccccatc gttttggcgt tgatcgcctc gcgccagtga ctctctttac
     8161 tgataccgta gctctcttca tagacaaaga gcttgatatc atcaatgtca tcaatatctt
     8221 cgccgccggt ggtattgatt tgcagaaact catcgcctga agtgtagtag cggaaaatct
     8281 gactaccgcc gcccagatcg atgtggctga cagcgactac cgtaaattcc tcacccggca
     8341 gcgcaatcag caattcatct tccagcaaac gaaacgctaa cgtgtcgagc gtaaagccac
     8401 tattgagatg aagtcccagc ggaccgcgag cgatagcagg cttattatcc ttgccaaaca
     8461 gacgctggaa aaaaccagac atattttatc cttaaaatag ttgccagcct tgcagtgagg
     8521 ctggcaaaaa aattactcgc cctgttggcg ttgcagtctt gctaatacat cctgggcgct
     8581 acttttattg ctaccgccaa ttccggcttc cgccagcttt tcgtcaaggt cgcgaccgtc
     8641 tgcgactttt tctaactgtg cggcagcatc caggcgagcc tgacgttcgg cctgacgcgt
     8701 ttgcaggcgt tttaaggatt ctgctgctgt cgaaacgctg gaagatgcgc caacggtaga
     8761 agtcgttaca gcctgttgtg cacgctgcat ggcttcagtg gctttaacga cttccatttg
     8821 ctgctcaaac tgagcgatac gctgcgctgt cgctgcaact gctttttcca cgccatcacg
     8881 ggagacttcc agattcgaca acacttgctc ttcagcggta atgagattct caaggcgcgc
     8941 gatttcttca gcaacttcgt taatcaacga cggattaacg ttcttgctta acgcttccag
     9001 cgtacgggct tccagactgg ctttgcgctc acgtaaatct ttcagcttat cgtgacttaa
     9061 tttcacccgc gccagcagat caacgcgaga ttttccggct ttatcgagtt cagctttagc
     9121 gtcacgaata tgctgttcca gcatgcgcac accctgggtt tcttcaatgg attcttccgc
     9181 ctgggagata aacgatttcc ccagcgtaaa taaacttttt aaaattccca taaatacccg
     9241 tccttgtaat tagtgtgaat attcttctgt aatttcagcc agatccaatg cgttatctac
     9301 cagcgaggtt atctcaagca ggatatcttc aagagaagat ttaagcgata acgccccgaa
     9361 aacaatgtaa tactcttctt gttgtacgct ggagattcct accgatgaca acggcatcat
     9421 cttctgattt cttaataaga aggtattaaa ttcatctgga ttgctgatac tactcaccgg
     9481 acaaataaag gtttcgataa tcatttggcg ggaagtaaaa agaatattga tttgcaaatc
     9541 gccataatca ttcattttaa taattaatgc gttctcgcta tttgttacat caatattttg
     9601 ttcaggtaca gtttgcagcg ccgtcgccag cgccaacgga ttccatgtca tatattattc
     9661 catatagatt aagtttaaat attaataaaa tgaatatttg caatacgtaa ttatcttacc
     9721 agctatagac aaaaaaaaac catccaaatc tggatggctt ttcataattc agaggaacta
     9781 gctgcgctga cgaaccgctt caaataagca aattccggtt gcaaccgaaa cgttcaggga
     9841 agaaacactt cctgccattg ggatgctgat caactcatcg caatgttcac gggtcaggcg
     9901 acgcatacct tcaccttccg cgcccatcac cagcgccagg cgtccggtca ttttgctttg
     9961 atagagcgta tgatccgcct cacctgccgt accgacgatc cagatattct cttcctgcaa
    10021 catacgcatg gtgcgcgcaa ggttagtcac ccgaatcagt ggaacgcttt ctgccgcgcc
    10081 gcaggctact tttttcgccg tggcgttgag ctgtgcggag cgatctttcg gcacaatcac
    10141 cgcgtgaacg ccagcagcgt ccgcgctacg caggcacgcg ccgaggttgt gcggatcggt
    10201 tacaccgtcg aggatcagca ggaacggttg atcgagcgaa gcgatcagat ccggcagatc
    10261 gttttcctgg tactgacgtc ctggcttcac gcgggcgata atgccctgat gcacggcacc
    10321 gtcgcttttc tcgtcgagat attggcggtt tgccaactgg ataaccacgc cctgggactc
    10381 aagggcgtgg atcagcggta acagacgttt atcttcacgg ccttttaaaa taaagacttc
    10441 ctgaaaacgt tcaggggcgc gctccagcag ggcctgcact gcatggatgc cgtaaatcat
    10501 ttcgctcatt aatgtactcg ttgtttacgt tttgcctgat gcactacgtt tatccggtct
    10561 acatgatccc tgcaatatat tgaatttgcg tgattatgta ggccggataa ggcgttcacg
    10621 ccgcatccgg catgaacaaa gcgcattttg tcagcaatct aaccctcttc tgtttgaaga
    10681 gggtaatgat cactctgcca cttttttctt cgccgcacgc ttcgctttgg tcgctgcagc
    10741 tattttctgc gttttcgccg atggcttttt cgcttttctc gcgtctttct tcgctgcttt
    10801 cggcttcgtt tttttctcac cgcggaaggc gctgtctggc tcaaagttta cctttttacc
    10861 gacctgacga cgcttgccgc cttttttacc tgcatcgcct tttttcgctt tctcgcgcgc
    10921 cgttttaccg acgttgcgcg gtgcgcgttc gctggagatc aggctaaagt cgattttgcg
    10981 ctcgtccata ttaaccgctt cgacgcgaac ttccacgcga tcgcccaggc gataagtctg
    11041 gccgctggat tcacccatca ggcgttgccc tacctggtca aagcgatagt agtcattgtc
    11101 cagcgaagag acatggacca gcccatcaat gaacaagtcg tccagacgga cgaagaagcc
    11161 aaagccagtg acgctggaaa ttacgccttt aaagacgtta cctacctggt cgagcatgaa
    11221 gtcacacttc agccagtcag acacatcgcg cgttgcttcg tcggcacgac gttccgccat
    11281 cgaacagtgc tgacccaatt gcagcatctc ttccatcgaa taatggtagc caccggtttc
    11341 agtggtgttg ccctgatgcc cctgctcttt cgccagcaga tacttaatgg cgcggtgcag
    11401 catcaggtca ggataacgac gaatcggcga agtaaagtgc gcataggact gcaatgccag
    11461 gccaaagtga ccacggtttt ccggatcgta aatcgcctgt ttcatcgagc gcagcagcat
    11521 ggtttgcagc atttctgcat caggacggtc ggcaaccgac tccagcagct ccgcgtagtc
    11581 gcgcggttcc ggcttgttac cgcccggcag ttccagcccc agctctgcca gcactgaacg
    11641 gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata cggaacagtg ccggttcttt
    11701 cgctttctca acgaaacgcg ccgccgagat attcgccaga atcatgcact cttcaattaa
    11761 cttgtgtgcg tcgttacgct gagtctgttc gatacgttca atacggcgtt cagcgttgaa
    11821 aatgaacttc gcttcttcgc tctcaaatga gatcccgccg cgttcttcac gggctttatc
    11881 cagcacttta tagaggttat gcaactcttc gagatgctta accagcgggg cgtactgctc
    11941 gcgcagatcc tgatcgccct gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg
    12001 agagctcatc accgcttcgt agaatttgta gccagtcagg cggcctttcg acgaaaccgt
    12061 catctcgcac accatacaca ggcggtctac ctgcggattg agcgagcaca gaccgttaga
    12121 gagcacttcc ggcagcatcg ggataacctg cgaagggaag tacaccgacg taccacggtt
    12181 acgcgcttct ctgtccagcg gcgttggcgg acgcacatag tagctgacgt cagcaatcgc
    12241 gacccataaa cgccagccgc cgccgcgttt tttctcgcag taaaccgcat cgtcaaagtc
    12301 acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg cgcaaatcga cacggcccgc
    12361 ttttgcttct tccggcactt cttctttcag cccagcaacc tgttgctcaa cagcctgcgg
    12421 ccagatgtac ggaatttcat gggtacgcag agcgatatca accgccatgc ccgtgcccat
    12481 attgtcgccc agcacttcga cgattttacc caccgctttg gtacggcgag tcgggcgttg
    12541 ggtcagttca acaaccacca caaagcccat ccgcgcgccc atgatctgat cgggcgggat
    12601 taagatatcg aagctcagac ggctatcgtc aggaaccaca aagccgacgc ccgcttcggt
    12661 aaagtagcga ccaacaatct ggctggtttt tggcaccagt acgcggacaa tacgcgcttc
    12721 acgacgacct ttacggtcag cacccagcgg ctgagccagc acctgatcgc catgaatgca
    12781 ggttttcatc tgctcgctgg agagatacaa atcatcttta cgcccttcaa cccgcagaaa
    12841 gccgtagcca tcacggtggc caataacggt acctttcacc aggtcgaggc gttccggcag
    12901 cgcatagcac tgacgacgag taaagaccag ttgaccatcg cgctccatcg cgcgcaggcg
    12961 gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc
    13021 acggctggcc ggtttttcac gtttggttaa atgttcgagg ataaattccc gactagggat
    13081 gggattcgcg tatttttcag cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc
    13141 tccgttgtca tctctgatga agattttcgt cactccacca gcaataattt ataaagcggt
    13201 tgattctctt caaccaaatc ggcaagcgtg tagttatcca gttccgtaag aaaactttgc
    13261 acggccttag aaagcgcctg tttcaaccga caggcaggtg taatgtggca aaactcactg
    13321 ctgcaattca ccagcgataa gggctccagc tcgcgcacca catcaccaat acgtatcgca
    13381 ctcgccggtt tacccaggcg aatgccgcca ttttttccac gaacagcagt cacgtagccg
    13441 gcacgactaa gttgattgat tattttgacc atatgattac gggagacgcc gtagacgtca
    13501 gtcacttcag aaatactggt catccgccct tctggcaatg acgccatgta gatcagcgca
    13561 cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg gaaatcggga
    13621 aaaaatacat ttatattgat gataaaccag ccacaagctg tgtcgctaat ttatttcagt
    13681 tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga
    13741 cacagcaatg tatcacactg ttagcccggc aggcaaaata tctgccaggc gtaccagaat
    13801 tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac gatccggacc ggtagagatg
    13861 atatcgatcg gtacaccggt cagctcttca atgcgcttga tgtagttcag cgccgcctgc
    13921 ggcaggccgc tacgatcttt cacgccgaag gtggattcag accagcccgg catggtttcg
    13981 taaatcggct ctacaccttt ccagtcgtca gctgccagcg gagtggtagt cacttcacga
    14041 ccatccggca tacggtaagc cacacagagt ttcacctctt tcaggccatc cagaacgtcc
    14101 agtttagtca ggcagaagcc agacagggag ttcagttgta ccgcacgacg aacggcaacg
    14161 gtgtccagcc agccggtacg acgacgacga cccgtagttg cgccgaattc gttaccctgc
    14221 ttgcagagga actcgccagt ttcatcaaac agttcagtcg ggaacggacc tgcacccaca
    14281 cgagtggagt aagctttgag gatacccaga acgtaatcaa cataacgcgg gcccaggccg
    14341 gaaccggtcg ccacgccacc agcagtggtg ttggaagaag ttacgtacgg ataagtaccg
    14401 tggtcgatat ccagcagcgt accctgcgca ccttcgaaca tgacgaaatc gccacgctga
    14461 cgcgcctggt cgagcaggtc agaaacgtca accaccatag aagtcaggat gtcggcaaca
    14521 gccatcgtat catccagaac tttctggtaa tcaaccgctt cagctttgta gtagttaacc
    14581 aactggaagt tgtgatattc catcacttct ttcagttttt cagcgaaggt ttctttgtcg
    14641 aaaaggtcgc caacacgcag accgcgacgt gccactttat cttcataagc aggcccgata
    14701 ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc
    14761 gcaacatgat aatcaaggat cagcggacat gcttcagaca gcagcagacg ctcacgaacg
    14821 gggatgccac ggtcttccag ttctttcatc tctttcatca gcgcggccgg agacagcaca
    14881 acaccgttac cgatgatgct agttacattc tcgcggagaa tacctgatgg aataagatgg
    14941 agaacggttt tttcaccgtt gattacgaga gtatggcctg cgttgtgacc gccctggtag
    15001 cgtacaacat atttagcccg ttcagtcaga agatcgacga tcttaccttt accttcgtca
    15061 ccccattggg tgcccagtac gacgacgttg ttacccattt ttcaaaatca ccgtttgctt
    15121 aaaaatggat tctaccatcg ctttttcaga gttacagcac tttttgcact caaaaatgac
    15181 ctcgatcagt ctgttttttg ttcagccaat cgttttcctc aacatgtagt agaccacaac
    15241 gcccgcaacc acaagtccac cgccaaaacg acgtaaaata ttatcgggca aattggtcat
    15301 cgcagagatc atcttcttcc atgccttcgg gtaaagcatc ggccctaaac cttccagtac
    15361 caaaaccagg gcaagcgcca gccagattgt cgaattcatt cattatcctt atagaaaaag
    15421 aaaaccaccg acatccctga ggatgcggtg gctttattga cctgtaccgc agtcgttata
    15481 ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag aaatcgctat ccgggctcat
    15541 gaccatcacg tcctgattgc cagagaagct gttctcataa gcacgcaggc tacggatgaa
    15601 tgcgtagaag tccggatctt tactgaatgc atcagcaaac agtttggctg cttcggcatc
    15661 gccttcaccg cgcatgatgc ggccctgacg ctcagcttct gccagcgttc tggtcacttc
    15721 atagtcggca gtcgcgcgca gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg
    15781 cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg
    15841 caggttgatc tgcttaattc gcacatcgac aacttcaata cccagcgccg ccatactgtt
    15901 cgggttgatg accggaactt tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc
    15961 aatggcgtta tctgccgccg gggtagtaac ttcatcttct gtacccgcag aaccggagtt
    16021 cagcgcgtca cgtacttcga gggtcagacg accacgggaa tcggtgacga tgtctttcac
    16081 gtccaggcga ccaatttcag aacgcagacg gtcagagaac ttacgtttca acagcacttc
    16141 cgcttgcgaa atgtcgcccc cccccgttgc caggtagtaa cggctgaaat cgctgatgcg
    16201 ccatttgatg taagagtcga cgatcaggtc tttcttctct ttggtcacaa agcggtcggc
    16261 ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc accgtttcaa tgaacggtat
    16321 cttgaaatgc agacccggct cataaaccag aggtttgttg tcatcgtcac gcagtacctt
    16381 accaaaacgc agcgtaatac cgcgctcacc ttctttgacg acaaagacag acatgtaaag
    16441 cactaccagc acgatgatga taatcgcgat aactgactta cgcatcgtta ttccccctga
    16501 cgctggtagt cgttacgctg cgcattggcg cggcgttggt ccataatatc gccctgactg
    16561 gtggacgacg tgttgcttgc tccactggtt gtggaggaag aggctggcgg cagacgcagc
    16621 agattgctgg caccgttatc gctcttcgcc gcaggggcgt taccaccttt cagcatctgg
    16681 tctaacggca gaaccatcag gttgccacct ttatcgttaa ccagcacttt gcgggtgtta
    16741 cccaacactt tttccatcgt ctcgatatac agacgctcgc gagtaatttc cggcgcggct
    16801 ttatattccg gcagaagttt agcaaaacgc gccacttcac cctgagcttc caggatggtc
    16861 tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc
    16921 tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg
    16981 gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg gacgagcagc ctggaagttg
    17041 acgtccagca gcgtgatacc catgtcatac ggacgaatcg tctcttccag ttcgcgctga
    17101 gtatcgctac gaatcacggt acgaccttcc gtcagaatgc ggtccatggt gtatttaccg
    17161 ataactccac gcagggcgct gtcggtagcc tgacgcaggc tgtcatccgg gctggtcacg
    17221 ctatacagat atttttccgg attggtgacg cggtactgca cgttcatctc aacgcgcact
    17281 acgttctcgt ccgacgtcag catcacacca gaagctgcca gttcacgcac ggcttccacg
    17341 ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt tcagacctgg ctcaaccaga
    17401 tggctgaatt taccaaagcg tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa
    17461 ccactggccg cccagataat gacaatcgct gccgctgcga tggtaacgac acgaccgcca
    17521 agctgcgggc gcgggccttg cgatgaactg ccaccgccag atccggtgcc tttaccgccg
    17581 cccagaccac cgagcttttt gctcagtttg cggaagatat catctaaatc aggtggccct
    17641 tgatcgctac cgcctttgtt tccatttccc tcagagttgc cgccaggttt gctgcttccc
    17701 cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc acgccatgtt tgtgctccat
    17761 atttgttatg cggtgatccc tgttgtcttt cgacgccaga ggatatgact ccacgcttca
    17821 gacgctacgc cgttagatca ggtaatcgat caacgccggt tcttgtttac agaggcgacg
    17881 ccagtcaacg atcggcatac gaacttgcag acttacgctg ccgtcctcct ccatccactc
    17941 tttttctatt gcctgaagct gataaaaacg acttctcaga cgcccttcct gcggtggcag
    18001 acgcaatgta tgctgcgcca cctcgccgga aagccgctcc gtcaaagcct gaaaaagctg
    18061 tggtatcccc gctccggtct gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc
    18121 ccgatcaata cgcggttcga aatcttccag catatcgatc ttgttcatca ccagcagggt
    18181 tgggatctcg tgagcgtcga tctcttcaag aaccgtattc accgcttcga tgttttcttg
    18241 tacacgcaca tccgccgcat caatcacatg cagcagcaat gtggcttgcc gcgtctcttg
    18301 taacgtggct ttaaatgctg ccaccagatc gtgcggcagg tggcgaataa accctacggt
    18361 atctgcaagt acggtttcac cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag
    18421 ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt
    18481 agatttaccg gcgttggtat atcccaccag cgaaacagta ggaacgtcgg ctttgatacg
    18541 cgattgccgc ccctgctcac gctgcttttc aactctttcc aggcgcgact gtatctgcac
    18601 gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa
    18661 accaatcccg cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc gtgtagccag
    18721 atggcgcagc tgcgccagct caacctgcaa cttaccctca tgggtacgcg cacgttgggc
    18781 gaaaatatct aaaataaggc cggtgcggtc gataacacga cactcgcaca aacgctccag
    18841 gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc
    18901 tttgacagct tccgcaattt caactgcttt accttcacct acaaaatact ttgggtgcgg
    18961 cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag agaccagaga
    19021 ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta aaatagatgt gtaccagtac
    19081 cgcctgctca ccagcatcat aacggtcaaa caagcgtata accctctaaa tagatcagcg
    19141 gggaacgcag gatcgctggc tccccgtgta aaaaaaacag cccgaaacct tattcggttt
    19201 cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct accatgatgg tagttactgc
    19261 tggtaccgcc accggcgttg ttactgtgat gagaaaccgg gcgagacggg acaacagtag
    19321 aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt caacaggatc acgaactgat
    19381 caaaagactc gatttgccct tgcagcttaa taccattcac caaataaata gaaactggaa
    19441 cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa agattgcccc ttagccattc
    19501 tctcttttcc ttatatgctt atttgtactt tgaacctttc gattctgaaa aaattgcgca
    19561 cgatacgtct caattgtaca cattcagcct gcgatagcac caacaacctg taatacttcg
    19621 tcacgcgcct gttctggttt ttcactgtca agccagtgaa ccccttccca accacgcagc
    19681 caggttatct gccgcttcgc caactgtctc gtggcgcaaa cacctcgata aaccatttca
    19741 tcgtatgaga tttcgcctcc aaggtaagac cacatctggc gataacccac gcaacgaatg
    19801 gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa
    19861 cctgaagcca acatctgatg aaaacgctgc tcaattcgtt gatggagcag ttcacggctc
    19921 gccggggcga tggcgaactg atgcacctga tacggtagag cgtctcctga cgtttgcgtc
    19981 agttccgtta aagttttacc cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt
    20041 ggatcatttg gatgaatcct tgctgccgca accggatcga tctcctgaag ttgacgatgc
    20101 aatgactccc aaccttgctc tgccgcctgt tgctcaattc tggcccgtac ttccgggtct
    20161 gccgacggta gcggcgacaa cccttccagc aatgccttga aatacaacat cgtaccgccc
    20221 actaacagtg ggatccgccc cgccgcggtg atatcggcca tttccgccag cgcatcgcgg
    20281 cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc
    20341 gcggcgagta actcttcagc gttcggcttc gccgtcccga tatccatccc tttgtaaata
    20401 agggcagaat caacgcttat caactctact ggtaaaattt tacgcagctc aatggctaac
    20461 gccgttttac cggaggccgt cggccccatc aaaaaaatcg ccttaggcag gctcgcctta
    20521 ctgatatcac tcatctttca gggcttttat cgccggatgt aaatcaacag attgtaacag
    20581 accacccggc ggcgttttca caagttgcgg acataaccgt tccacgtccg ccagcagggt
    20641 tatggcctgt gccattgacc actgcgcatg ttcgctcatc agatttcgtg caatccactg
    20701 cgcaatattg ccaggttcga atacggactg cttcgctagg tagcctatca gttcaggaat
    20761 caagatttgt aaattttgtt ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg
    20821 tgcatctgac tggaaatcaa tacccaattc cgccagggca gactgcgctt tttctaatgc
    20881 cgatttttct tcgccagaaa cttttagccg caacggaatc agcaacggct gggcgcaaac
    20941 gggcgcttca cccggcgtca gttgtacctg acgcagccaa cgttctgcca ctggcaaggc
    21001 taaaagtgaa atgttgccgt cgcgctccag caacgcacag tcggaatgga cgatagtcag
    21061 tacccgacca aaactctgac tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt
    21121 tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga tacacttcac cttgctgttt
    21181 ctggtaacct ggctgcgcat tcggccaggg ggcagccgga cgactacctg atgctggcgc
    21241 aggagtgtag cgcggagcta ctggctcacg aactgccggt tcagcaaagt gattgcgccc
    21301 cgccgccacg cggttttccg gaatgggacg cggtgcaggt tggggttcat cgtccagcgg
    21361 tagcggcgtt tccagttgct gttgtagcac gctcagcacg ccctgataga taaagtcatg
    21421 caccagacgc gactgatgaa aacgcacttc gtgtttggcg gggtgcacgt tgacgtccac
    21481 ctgatgcggg tcgatctcca gatacaacac aaatgcaggt tgctgatcgg cccccagttt
    21541 gtcttcacag gcctggcgga tcgcgtgatt gatcaggcga tcgcgcatca tgcgaccatt
    21601 cacgtagcaa tactgaattt ccgccagtgc gggcgtggtg tgatttggat cggccaccca
    21661 gccgcgcagc gtgagatcgc cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc
    21721 ggtgccacaa atcgcgccta agcgccgttc tttttgcccg ccttccggca ccgcgcggta
    21781 ctggcgtacg attttgccgt tatgcgacag gttgatcgtg acgtcgaaac gcgccagtgc
    21841 gatgcggcgg atgatttcat cgatatggtt aaattcggtt ttctcggtgc gcaggaattt
    21901 gcgccgcgcc ggggtgttgt agaacaggtc cagtacctcc agcgtcgttc ccaccggatg
    21961 cgccgccggt ttaaccgtca cgtccatatc gcgcccttcg gcataggcct gccaggcttc
    22021 ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa accgaactga tactcgccag
    22081 cgcctcaccg cgaaagccca ggctgataat ggcttcgaga tcgtccagag aggcgatttt
    22141 actggtggca tgacgcgcca gcgccagcgc cagctcgtct tttttgatac cacagccgtt
    22201 atcacgaatg cggataagtt tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc
    22261 acctgcatcg aggctgtttt ccaccagttc tttgactacc gacgcaggtc gctcgaccac
    22321 ctcacctgcg gcaatctggt tcgccagttg tggcggtaag acctgaattg gcatcaatcg
    22381 tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc
    22441 accctgcggc gcagattgca tcggatgcgc aaggaaataa ttgcgcaggc ctttgtaaat
    22501 ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt
    22561 gctgataaaa ccggtttcga ccagtactga tgggatatcc ggcgagcgca gaacgccaag
    22621 gctggcgtgt tctggtcgac gtttatgtat ttcgccaatg cgttgcaact gactgatcat
    22681 actggtcgct acatcatacc ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc
    22741 ctggcttaaa taggggtcag actgactgtt cgccagcaca tcacccgccc cgcccagcag
    22801 ctccgactgt ttctcgtgct gctccagcca gctggccatt tcgctgttgg cgcgacggtt
    22861 agagagcacc catacggaag cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat
    22921 cgacacgagg aaattggcgt tttgcttacg tgccacatcg ctgcgcccca tcaccgagat
    22981 aaagtaatcc ccgtcacggg ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt
    23041 acgcaattta cgcgcgatgg cgatggtgac atttttctcc cgcgtaccac cggggccgat
    23101 agcgccaggg tcctgaccgc cgtgtccggc atcaatagcg atgataattt tatcgccatt
    23161 gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta ctgctgataa cacccgtagt
    23221 gcggttactt tccgttttaa acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac
    23281 cgtaggcgtt tcaacgcgtt tcacaaccac aggcggcgga ggaggcggtg gcggcgcatc
    23341 ggcgttaatc gtaaagacga cagtgtaatt gctgccattc tgccgcttca ccgcttcggt
    23401 tttaccattt tcggtaagat cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc
    23461 ggagcgaatc gccttcacca gattattacc gctgaacaac aacggcagtc cctgaatcac
    23521 gcccgtttgt ttgatatcga gcgccacggt gcgtttgctt tgatggctaa acgcataatc
    23581 aggatcgcca ataaaactca acgttatccg cgcctgttgg ttgccgttag aaacctgaat
    23641 atcagagagc gtcgcggcac ccacctgcgc gcacagcagc agcaacgtcg ctaccaacca
    23701 atttctgatg cgatacatca tcccaccacc tttcaaaggt taaccggcta aacgtgccag
    23761 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    23821 atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc
    23881 caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc
    23941 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg
    24001 ttcgaccagc gtataagtgg ggcttttgac attaccctga tggcccaaag cctgtaaaaa
    24061 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    24121 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    24181 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    24241 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    24301 cagctgccgc accgtgcgcg acacagcctg cacaggctgc atcatacggc gacagttttt
    24361 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg cttgccatgc
    24421 ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg
    24481 cacctttcag caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc
    24541 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    24601 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    24661 catcccacaa catcggtttg cgaaaattct caactttttg cagggctttt ttcccccact
    24721 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    24781 gagagtccat agtcagttca tgcaccatca attctggtcg tgcagtcagc agcggcgcaa
    24841 tgttctcact gcgggtcagt actcggacta aaccagcacc agcacgtagc gccgcttccc
    24901 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataatcacc agccgcccgt
    24961 gatcgccttt atgcgaagtc gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg
    25021 aaaaccgctg aattttcgtc tcctgacctg ccagccagct atccagcccc agtgagtcaa
    25081 aatgcagttg tcctgtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    25141 taaaagtgat ggtgtgatcg gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca
    25201 gcaggccgga agggatatca accgccacaa tcggcgcagg atgggaatta gcgtggtcga
    25261 ttaactggct aatggattcg cggggtgctt gctgcaagcc ggtaccgagc agcgcatcaa
    25321 caatcagagc taccgactcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt
    25381 ttaaccatgc ctcgcgtgcc agcgcggcct cttccggcaa cggtttatcg ctctcctggg
    25441 ccaacaacgt gacctcaatg ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc
    25501 cgccgttatt accatgaccg cacaacacca gccagtggcg ggcgtcagga tacgccgaac
    25561 gacacacctg gaatgcggcc tcgccagcgc gaagcatcag ctcatagagt gtgagcccca
    25621 gcgcatctgc cgcctcgcgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg
    25681 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    25741 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    25801 ttaccgatac cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat
    25861 accacggcga aatggactgg atggcacgtc acggtatgct gcgcgctcgc ccccatgagt
    25921 tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    25981 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    26041 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    26101 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt
    26161 tagctgaaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    26221 ccggttcgtt cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac
    26281 cagtcgagga aggatgcggc aaatgcgtgg cctgtatgac gatttgcccg accggtgcca
    26341 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaactgg
    26401 aaggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg
    26461 actgccagct tatctgcccg tggaatcgct attcacaact cactacagaa gacgatttca
    26521 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    26581 agtttttaaa agtcacggaa ggttcggcga ttcgccgtat aggtcacctg cgctggctgc
    26641 gtaatatcgc cgtggcatta ggcaatgcgc cctgggatga aacgattctg gcggcgctcg
    26701 agagtcgcaa aggtgagcac ccacttcttg atgagcacat agcgtgggcg atggcgcagc
    26761 aaatcgagag gcgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg
    26821 ttcgggtaat tgaaaaaggg ttaccgcgtg acgcctgatt catccacagc ttgtgaataa
    26881 aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa
    26941 cgaaacctta atttttaaat tatctatcaa taggttaaaa aaatactatt caccgtgcga
    27001 agtttctttg aaattaagat tacggcctga ggctgtggat aactctgttt acaagagttt
    27061 gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt ttattgagag
    27121 aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    27181 tctaccaact gagctattcc cgcttgggtg gtctgtgcct tacagcactt tcaaattttg
    27241 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
    27301 tgagctattc ccgcttgggt ggtctgtgct ttgcagcact tttcaaattt tggagcggga
    27361 aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat
    27421 tcccgcgtac tacttaattt tgctttcgta attttcgcat ttctgcgtcg ttacgggagg
    27481 cgcattatac gagaaatctt tccctctgca acccccctaa aaattttttt ttgagttttt
    27541 atctcaagtg ctgaaattat cagcaagacg ttcaatttag cgtcaaaatc acgccgccgc
    27601 aagtggattc cggcgcttac gtgaccagca aaaattttac agcttgataa aatgctcgcg
    27661 gtagtaagcc agctccgcca ccgattcacg gatatcatcc atcgcctgat gcgtcccctg
    27721 tttggtaaaa ccatcaagaa tttccggctt ccagcggcgc gccagctctt tcagggtgct
    27781 gacatcgaga taacggtagt ggaagtaggc ttccagctcc ggcatgtatt taaacaggaa
    27841 gcgacgatcc tgaccgatgc tgttaccgca aatcggcgat ttacccgcag gcacccactg
    27901 ttttaaaaat tcgagcgttg ccagttcagc ttcacgatcg cccatcgtgc tcgctttcac
    27961 gcgctccacc agcccgctgg cggtatgggt gcgcacgttc cagccatcca tcagcgccag
    28021 ctgttcatcg gactgatgta ctgcaatggt cggcccttct gccagaatat tcagattggc
    28081 atcggtcacc agcgtggcaa tctcaataat gcgatcgcgc tcgggatcca gaccggtcat
    28141 ctcaagatcg atccaaatca ggttgttttc attggcactc atgctatttt ccacccattc
    28201 acagtgacta tattgatcca caattgcgtg tatcatagat gttttgccca tcaggggcga
    28261 ccaggagtca gtacgattga gtaaaaataa actctccaaa ggccagcagc gccgcgtgaa
    28321 cgccaatcac cagcgtcgtc ttaaaacgtc taaggagaag cccgactacg acgacaatct
    28381 gtttggcgag cctgatgaag gtatcgtcat cagccgcttt ggtatgcacg ctgatgtgga
    28441 atccgccgat ggcgacgttc accgctgcaa tattcgccgt accatccgtt cgctggtgac
    28501 cggcgaccgc gtagtctggc gtccgggtaa accggcggcg gaaggcgtaa atgtcaaagg
    28561 gatcgtggaa gcggtgcatg agcgcacctc agtgttgacg cgtccggatt tctacgacgg
    28621 cgtgaaacct attgccgcca acatcgacca gattgtcatt gtctccgcca ttttgccgga
    28681 gctgtcgctc aatattatcg accgttacct ggtggcctgc gaaaccttgc agattgagcc
    28741 gattattgtg ctcaacaaga tagacctgct ggacgacgaa ggcatggcgt tcgtcaacga
    28801 acagatggat atctaccgca atatcggtta tcgcgtattg atggtttcca gccatactca
    28861 ggatgggcta aaaccgctgg aagaggcgtt gaccgggcgc atcagcattt ttgccgggca
    28921 gtctggcgtc ggcaaatcca gcctgctgaa cgcgttgctg gggctgcaaa aagagatcct
    28981 gaccaacgat gtctctgaca actcggggct tggtcagcac accacgaccg ccgcacgcct
    29041 gtatcacttc ccgcacggcg gtgatgtgat tgattcccct ggtgtgcgtg agttcggcct
    29101 ctggcacctg gagccggaac aaatcactca gggctttgtc gaattccatg actatttagg
    29161 tctgtgtaaa tatcgcgatt gcaaacacga taccgatccg ggctgcgcta tccgggaagc
    29221 ggttgaggaa ggaaaaatcg cggaaacccg tttcgaaaac tatcaccgta ttctggaaag
    29281 catggcgcag gtaaaaacgc gtaaaaactt ttctgatacg gatgactgac aattaagcta
    29341 accgtcgtta gaatcgtccc ctttttttca ggatccggca tttaggccgg atcaggaacg
    29401 acaaaacaat ggcctggagg ctaccttgtt aaattcattt aaactttcgc tacagtacat
    29461 tctgccgaaa ctatggctta ctcgcctggc gggttggggc gcaagcaagc gggcaggatg
    29521 gctgacaaaa ctggttatcg atctgttcgt taaatactac aaggtcgaca tgaaagaggc
    29581 gcaaaagccg gacaccgcca gctaccgcac ctttaacgaa ttctttgtcc gtccgctgcg
    29641 tgacgaagta cgcccaatcg ataccgatcc gaatgtcctg gtcatgcctg ccgatggcgt
    29701 tatcagccag ttgggtaaaa tcgaagaaga taaaatcctg caagccaaag gccacaacta
    29761 cagcctcgaa gccctgctgg caggcaacta tctgatggcg gacctgttcc gcaacggtac
    29821 gtttgtgacc acttacctct ccccgcgtga ctaccaccgc gtacacatgc cgtgcaacgg
    29881 tattctgcgt gagatgatct acgtgccggg cgatctcttc tccgttaacc atctcacggc
    29941 tcagaacgtg ccgaatctgt ttgcccgtaa cgaacgcgtg atctgcctgt tcgataccga
    30001 atttggccca atggcgcaga ttctggtcgg ggcgacgatt gtaggcagca ttgagacggt
    30061 ctgggcgggc accattacgc cgccgcgcga aggcatcatc aagcgctgga cctggcctgc
    30121 cggggaaaac gacggttctg tggcgctgct gaaaggccag gaaatgggtc gctttaaact
    30181 tggctctacc gttatcaacc tgtttgcacc gggtaaagtg aatctggttg agcaactgga
    30241 aagcctgtct gtcacgaaaa ttggccagcc gctggcagta tctaccgaaa cctttgttac
    30301 gccagacgct gaacccgccc cgcttcctgc tgaagagatc gaagcagaac acgacgccag
    30361 cccattggtt gacgataaaa aagaccaggt ctaatccatc aaaggaaacg ctgacgtgcg
    30421 cctgattatc acttttctga tggcctggtg cctcagttgg ggggcgtacg ccgcgacggc
    30481 ccccgatagc aaacaaatca cccaggaact ggagcaggca aaagcggcga aacccgcgca
    30541 gccagaagtc gtagaggcac tccagtctgc cttaaatgcg cttgaggaac gaaaaggttc
    30601 ccttgagcgc atcaagcaat atcaagaagt cattgataat tatccgaaac tctccgctac
    30661 tctgcgcgca caattaaaca acatgcgtga cgagccgcgc agcgtgtcgc cggggatgtc
    30721 taccgacgcg ctgaatcagg aaattctcca ggtcagcagt cagttgctgg ataaaagccg
    30781 tcaggcccag caagagcagg agcgcgcccg cgagattgcc gattcgctga atcaactgcc
    30841 gcaacagcaa accgacgccc gccgtcagtt aaatgagatc gagcgccgcc tggggacgct
    30901 taccggcaat actccgctca atcaggcaca aaatttcgcg ttgcagtctg actctgcacg
    30961 tcttaaggcg ctcgttgatg aactggagct ggcgcagctg tctgccaata accgccagga
    31021 attagcgcgc ttgcgctctg agctggctga aaaagagagc cagcaactgg atgcgtattt
    31081 gcaggccttg cgtaatcaat tgaacagcca acgccagctt gaggcagagc gggcgctgga
    31141 aagtaccgaa ttactagcag aaaacagtgc cgatttgccg aaagatatcg tcgcgcaatt
    31201 caaaattaac cgcgaactat cggcggcgct gaatcaacag gcgcagcgga tggatctcgt
    31261 tgcctcgcaa cagcgtcagg ctgccagcca gacgttacag gtccggcagg cgttgaatac
    31321 gctgcgtgaa cagtcgcaat ggctgggatc gtccaatctg ctcggtgaag cgttgcgggc
    31381 gcaggtggca cggctgccgg aaatgccgaa accacaacag cttgataccg aaatggcgca
    31441 gttgcgtgtg caacggttac gttatgagga tctgcttaat aaacagccgc tgctacggca
    31501 aattcatcag gccgacggtc agccgctgac cgctgagcaa aaccgtattc tggaagcaca
    31561 gctacgcact cagcgtgagt tgctgaactc attgttgcag ggtggcgata cgctactgct
    31621 ggaactgacc aagctgaaag tctccaacgg gcaactggaa gatgcgctga aagaagtgaa
    31681 cgaagcgacc caccgctatc tgttctggac ctctgacgtg cgcccgatga ccatcacctg
    31741 gccgctggaa atcgcccagg atctgcgtcg tctcatttcg ctggacacct tcagtcagtt
    31801 gggcaaagcc agtgtgatga tgctgaccag caaagagacg attttgccgc tgtttggcgc
    31861 gttgattctg gtcggttgca gtatttactc gcgccgctat ttcacccgtt ttcttgaacg
    31921 ttcggcggcg aaagtcggca aagtgactca ggatcacttc tggctgacgt tgcgcactct
    31981 tttctggtcg attctcgtcg cgtcaccgct gccggtgctg tggatgacgc tgggttacgg
    32041 cttgcgcgag gcgtggcctt atccgctggc ggtcgcgatt ggtgatggcg tcacagccac
    32101 cgtgccgctg ctgtgggtag tgatgatttg cgctaccttt gcccgcccga acggcttgtt
    32161 tatcgctcat tttggctggc cgcgcgaacg tgtttctcgt gggatgcgct actacctgat
    32221 gagcatcggg cttattgtgc cactgattat ggcgctgatg atgttcgata acctcgacga
    32281 ccgcgaattt tccggttcgc tgggacggct ttgctttatc ctcatttgcg gtgcgctggc
    32341 ggtggtcacc ctcagcctga aaaaggccgg gatcccgctg tatctcaaca aagaaggcag
    32401 cggcgacaat attaccaacc atatgttgtg gaacatgatg attggcgcgc cactggttgc
    32461 cattctggcg tcggcggtgg gttatctggc aacggcacag gcgctgttag cgaggcttga
    32521 aacctcggtt gccatctggt tcctgctact ggtggtttat cacgttatcc gccgctggat
    32581 gctgatccaa cgacgcaggc tggcgtttga ccgggcgaag catcgccggg cagagatgtt
    32641 agcgcaacgt gcgcgtggcg aagaggaagc acatcatcac agtagcccgg aaggggcaat
    32701 tgaagtcgat gaaagcgaag tcgatctcga tgccatcagt gcgcagtcct tgcggctagt
    32761 gcgctcaatt ttgatgttga tcgccctgct ttcggtcatt gtgctgtggt cagaaatcca
    32821 ttccgctttc ggcttcctcg aaaatatttc gctgtgggat gtcacctcca cggtacaggg
    32881 cgtagaaagt ctggagccga ttaccctcgg tgcggtgctg attgccattc tggtgtttat
    32941 catcaccacg cagctggtgc gcaatctgcc cgcgctgctg gaactggcga ttttgcagca
    33001 cctggattta acgccgggta ccggctacgc catcaccacc atcaccaaat atctgctgat
    33061 gctgattggc gggctggtcg gcttctcgat gattggtatt gagtggtcga aattgcagtg
    33121 gctggtcgcc gcgctcggtg ttggtctcgg ttttggtttg caggaaattt tcgccaactt
    33181 tatctctggc ctgatcatcc tgttcgaaaa accgattcgc attggcgata cggtgacaat
    33241 tcgcgatctt actggtagcg tgacgaaaat taatacccgc gcaaccacca tcagcgactg
    33301 ggaccgtaaa gagataatcg tgccgaacaa ggcgtttatt accgagcagt ttatcaactg
    33361 gtcgctctct gactcggtca cgcgcgtggt gttgacgatt ccggcccctg ccgatgccaa
    33421 cagtgaagaa gtgacggaaa tcctgctcac cgcagcgcgt cgctgctcgc tggtgatcga
    33481 caacccggca ccggaagtct tcctggtgga tctgcaacag gggattcaga ttttcgagct
    33541 gcgtatttac gccgctgaga tgggtcaccg tatgccgcta cgccatgaga tccaccagct
    33601 gattctggct ggcttccatg cccacggtat cgatatgcca ttcccgccct tccagatgcg
    33661 tctggaaagc ctcaacggta aacaaacggg gagaacgctg acgtctgcgg gcaaaggtcg
    33721 tcaggcggga agtttgtaat gcatgaagct gccaaatgat gtcaacgtgg ctcatttagc
    33781 agcttaacat catcttcagt tatcaccggc cctggagagt atcatccata cgcattactt
    33841 cctgacaact catcgccgga gagacgatga gttgcaggat ttcatatgat cagtcgagag
    33901 ctaactcagg tttattcgcc atgcgactgc aatagttctg gtaaatcgcc atcgctaaca
    33961 gcgagaagaa gacagggccg ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa
    34021 ccacaggctg aataatggtg aagacgttcg caaatgtcac caccagaacg acaaccaccg
    34081 tcgcaatcat tgccgacata cgcgttttga aaatcacaaa tggtctgtcg agatcctgac
    34141 gtgctttaaa gaatgggaaa gccagcgcga ggaacaggta aggaagcgtc atagacacgt
    34201 tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt accgccacca aacgaaacca
    34261 gcaggatgaa gacagtaacc aacccgcact gcatccacat agcgatagac ggcatcccca
    34321 tcgcattcag gcgcgtcatc ggttccggcc acaacgcttt cggcgtcccc tggatgatgg
    34381 ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt ataagcgagg aacatcgaaa
    34441 gtccggtaat acgcgcaaac catacgccca gcgacaatga cgcttcaggt gacaaatgca
    34501 gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac ataggtaata ttgccgaggt
    34561 taacggaacc attacttaat acctgctgcc agtttgtgct gacgccccat aaaaatattg
    34621 ccagcgaata accgattgaa ataacaatag cggcaaaaac aatacctttg gcaaagttct
    34681 tttctggatt ttccgtttta tcgaccagac caccaaccgc ttcaattccg ccataggcaa
    34741 aaatagcaaa taccacaaac gatagcattg ccagaccgga ctgataacct gggttcggtg
    34801 atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt taataacaaa atagtaatgc
    34861 ttactaacag caatactaaa ttcagacaca ttactgcaat accgcccacc gcagtaatgc
    34921 gggcaatttt attaatcccc tttgaagcaa cgacggtgac cagaatcatc catgccactg
    34981 ccagcagacc aaccacctgc gtaggctcca gtccggcaat acgccagtgc tgggtcatgt
    35041 cgctaccata gaggaatgtt gagaacggta cccaaacttt cgcggaggtg ctcaccatcc
    35101 agatgatata agaggaaaac cacataaacg taccaatgaa ggcaaaacgt ggtccgacac
    35161 tattattcat ccaggaatag ataccgcctt cttctttgcg ataagcagct cccatttcag
    35221 ccatcattaa ggcgaatgga ataaagaata acaatgcaga aaatatataa aagggaatcg
    35281 cactataacc cattaagtaa taagccgatg ggctattggc aaatccaaat acggaagtaa
    35341 agatcatcaa tatgagtcct atcagactca tcttttttat cgtgtgaggc atgaaaccat
    35401 ccttcatttc agcgcgtgta taaaacgcgc ccactgaact gcactgccca acattgagcg
    35461 ggcagcgtaa aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa
    35521 ttgtggcctt tagcggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt
    35581 cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac
    35641 ttcagccagt gtctccgcgc ccagcgccaa catcaccaga cgatcaacac ctaatgccac
    35701 gccggaacag tcaggcatac cgactttcaa ggcttcaatc agattctgat caatggggtg
    35761 ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc
    35821 acgggcatcc gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc
    35881 aaagcgttca gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc
    35941 tggaaagtgg tacacaaagg tcggtttttc tttaccaata tttggttcta cgccaaaggt
    36001 aaacagcaat tgtagcaacg tgtcgcggtc ttcttcggta tcagcaacat tgctcaaatc
    36061 cagtttcgct gccacttccc gcagttgcgt tttgtcggca gagagcgggt caatttccag
    36121 ataacgcaag aaagcttgtt gataagaaag gctttctgct gccgggcagt ccagcacctg
    36181 ttgtaagaga tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca
    36241 ctcaagcata gtgaactcag ggttgtgata acgccccatc tcttcattac ggaaactgcg
    36301 gcacagctgg aataccggcc cgcaaccggc aaccagcagg cgtttcatat ggtattccgg
    36361 gctggtcatt aaccagagat tcatcccctg cgaatgcccg gggccaacga aacgtgtctc
    36421 aaacgggacc aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc
    36481 cagcactcca cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa
    36541 taagttagga atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc
    36601 agtttttgac aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag
    36661 gaataaaaca acggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa
    36721 ttaatcatct tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa
    36781 tttcagactt atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt
    36841 cgcagaccgt aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc
    36901 tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg
    36961 ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc
    37021 tcaaaagtat acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc
    37081 gcgcaggatc atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg
    37141 ttgtgtgagc aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa
    37201 ctggaactgt gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc
    37261 ttcggcggca tgaaaatcga gcgtacctgg ttcgccgccg ataagaccgg cttccatatg
    37321 ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat
    37381 ttcgtgctgg atattctggt tgatgatggt catgttcgcg gcctggtagc aatgaacatg
    37441 atggaaggca cgctggtgca gatccgtgct aacgcggtcg ttatggctac tggcggtgcg
    37501 ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg
    37561 gcgctaagcc acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt
    37621 ctgccaggtt ccggtatcct gatgaccgaa ggttgccgcg gtgaaggcgg tattctggtc
    37681 aacaaaaatg gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc
    37741 gagccgaaaa acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg
    37801 cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg gtgatgtggt ttatctcgac
    37861 ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg
    37921 aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac
    37981 tacaccatgg gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc
    38041 gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa accgtctggg ctccaactcc
    38101 ctggcggaac tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca
    38161 actgccggta atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt
    38221 ctgaaagatc tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg
    38281 ggcctggcta tggaagaagg ttgcggtatc taccgtacgc cggaactgat gcagaaaacc
    38341 atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacttcc
    38401 agcgtgttca acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct
    38461 gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg
    38521 gacgaaggtt gcaccaagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc
    38581 gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac gctaccgcca
    38641 gctaaacgcg tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag
    38701 aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc
    38761 ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc
    38821 attactggat gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg
    38881 gtcctgccgt atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact
    38941 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
    39001 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
    39061 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
    39121 cccggcgcag atggcgaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
    39181 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
    39241 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
    39301 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tactgctccg aagtctgccc
    39361 gaaacacgtc gatccggctg cggccattca acagggcaaa gtagaaagtt cgaaagactt
    39421 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
    39481 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc
    39541 tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
    39601 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
    39661 ttattgtgat cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt
    39721 ttgaactggc accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
    39781 caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
    39841 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg
    39901 gtattctggg gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg
    39961 atcctgctgg tgggtattct gctgccgctg gggctgtttc cgggtgatgc actgagctac
    40021 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
    40081 gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatc
    40141 cacgtacctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg
    40201 ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg
    40261 gccgttttgt atggaaacca gaccctatgt tcaaaacgac gctctgcgcc ttattaatta
    40321 ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa
    40381 ttaccccgct tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg
    40441 gtaaacctta ttactttacc tgggggtatg cggacatcgc caaaaagcag cccgtcacac
    40501 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
    40561 acgctattgc tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac tggcctgaac
    40621 ttaccgctaa acagtggaat gggatcacac tattacatct cgcaacctac actgctggcg
    40681 gcctgccatt gcaggtgccg gatgaagtga aatcctcaag cgacttgctg cgcttctatc
    40741 aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg
    40801 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa
    40861 ctcgtgtctt ccagccactc aaactcaacc atacgtggat taatgtaccg cccgcagaag
    40921 aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt
    40981 tagatgctga aacttatggt gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa
    41041 gcaatatgaa tccccgtgat atcaacgaca aaacacttca gcaagggata caactggcac
    41101 aatctcgcta ctggcaaacc ggcgatatgt atcagggtct gggctgggaa atgctggact
    41161 ggccggtaaa tcctgacatc atcgttaacg gcagtgacaa taaaattgca ctggcagcac
    41221 gccccgtaaa agcgattacg cctccaactc ctgcagtacg agcatcatgg gtgcataaaa
    41281 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
    41341 tcgtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga
    41401 ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt
    41461 ttccttgctg tcatctacac ttagaaaaaa ccagtaagga aacatttatg cgcctgctcc
    41521 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
    41581 cgcgtggcgt gaccgtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
    41641 agattgcccg ttttgatcac cgctttgaac gtggactgga aaaagtcacc gcaacataca
    41701 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
    41761 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
    41821 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
    41881 accgccatgc gctggtttgc ggaccggacc gcgactacct gtggatactc tcccgcacgc
    41941 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
    42001 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
    42061 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
    42121 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
    42181 aagccgtccc tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca
    42241 tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc
    42301 ctacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
    42361 gctccggaga attggggccg tccccggtga aaagacgcgt ttgcaggtcg tcctgcaaag
    42421 ctgatggttg aacggatatt ttgccaggtg gcgggggaat ttcaactatt acctttgtga
    42481 ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta ccgttattgc ttattgctgc
    42541 gctttcgttg ctgcaccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
    42601 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
    42661 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
    42721 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
    42781 agaaaaatta gctggcagcg cgggagatgg agttgccgag atgtttgatg tcttcgccga
    42841 aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
    42901 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
    42961 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
    43021 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
    43081 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
    43141 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
    43201 cctgatccag cagccattta gcgttgtcgc caattgcttt tgcgtcagca gacagctgct
    43261 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
    43321 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
    43381 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
    43441 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
    43501 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
    43561 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
    43621 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
    43681 gcggacgaaa aactgttggc cggacgcaac gccaaaaagc tgtttgccct gcgtgtgccc
    43741 cgctcattta tcgatcgcat ggagaaaggc aatccggacg atccgctttt gcgtcaggta
    43801 cttacctcgc aagatgagtt tgtcgtcgcg cccggattct ccaccgaccc gctggaagaa
    43861 cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
    43921 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
    43981 aatcagggca acaagcgtaa ctggcaaact gcacttgagt atgttgctgc gcatccggaa
    44041 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac
    44101 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagtcgt
    44161 ctgccgattg tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt ttcacattct
    44221 acgctgcaaa tcttgctggt gaatcacatc aaccatgcca atgagataga tgaaacattc
    44281 cgtcaggcga tggctaagtt gcgccgggcg ggcgttactt tgctgaacca gagcgttctg
    44341 ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat
    44401 gcaggcgtga tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt
    44461 atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact ggtgtcggga
    44521 tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat
    44581 ctccagctac gccagcagta actgattaaa cagggaagtt taatctttgc ccgttacata
    44641 acctttaatt attcatatac attgctgttg ttactcttta tataaccaac accaataatt
    44701 aacctttctt ttctgtttat atagagtgag tacggctatt tatgttattt taacaactat
    44761 tactaccaag cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg
    44821 ttagggagaa cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag
    44881 agtaataatt tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg
    44941 ttctttatgt ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat
    45001 caaaagcata aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc
    45061 attaagaaaa tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat
    45121 attaatcgtc gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc
    45181 gtattaacgc tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa
    45241 atgctggcgc gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta
    45301 cgtattacct cactgttaga tttcctgccg ttatatgacg ttgactgcca gcaaaatggc
    45361 aatctggaat atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat
    45421 ctggcggtgc tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg
    45481 gtgaaaacgc gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt
    45541 gatttcagcc cacgactgaa aaaactcgtg ggtgtgccat gtcaggtcta tgttcgcacc
    45601 gtagcggcga ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac
    45661 ttacgtgttc aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa
    45721 tgcgaggctg gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga
    45781 tgcggcgtaa acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc
    45841 ttatgtaggc ctgataagcg aagcgcatct ggcaattata cgtttgtcac catcacatca
    45901 gttcgggcac ttatatacct ggccgataat ctggctatcc gtcgggacaa aactggacaa
    45961 cattccctgc gacgggctac tgatgccata aatcacgtta ccgcccattg cagccgcctg
    46021 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
    46081 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
    46141 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
    46201 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
    46261 cataatcaat gagctggacg ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga
    46321 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaggtg
    46381 cagggcaatt acatcatgcc gcccatgcca cccatgccac ccatgccgcc cataccgcca
    46441 gcagcgccta agtcagctgc atcgtttttc ggcaggtcgg taaccatgca ttcggtggtg
    46501 atcatcaggc cagccacaga agccgcgtac tgcagagcag aacgggttac tttggttggg
    46561 tccaggatac ccatgtcgat catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag
    46621 ttgccgtcgc cgcctttaac ggtgttagca acaacagacg gttcttcgcc gcagttcagg
    46681 acgatctgac gcagcggagc ttccattgca cgcagtgcaa ctttgatacc cacgttctgg
    46741 tcttcgttct gaccacgcag gtcagccagt ttagacgcta cgcggatcag cgcaacacca
    46801 ccaccagcaa ccacgccttc ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg
    46861 cgtgcttttt tctctttcat ttcaacttcg gtagcagcac ctactttgat aactgcaacg
    46921 ccgcctgcca gtttcgctac gcgctcctgc agtttttcac ggtcgtagtc agaagttgct
    46981 tcttcaatct gctgacggat ctgagcaaca cggccctgga ttgcagcttc ttcgcccacg
    47041 ccatcgatga tggtggtggt gtctttgttg atcacaacgc gtttagcctg acccaggtct
    47101 tccagggttg ctttttccag ctccataccg atctcttcag agattacggt accgccagtc
    47161 agggttgcga tatcctgcag catagcttta cgacgatcgc cgaagcccgg agctttaact
    47221 gcagcaactt tcacgatgcc acgcatggtg ttaacaacca gagttgccag cgcttcgcct
    47281 tctacatctt cagcgatgat cagcagcggt ttgcctgctt tggcaacggc ttccagaacc
    47341 ggcagcattt cgcggatgtt ggagattttc ttgtcagcca gcaggatgaa cgggctttcc
    47401 agttctactg cgccagtttc cggcttgttg atgaagtaag gagacaggta gccacggtcg
    47461 aactgcatac cttcaaccac gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg
    47521 ataacgcctt ctttaccgac tttgtccatc gcttcagcga tcagtttacc tacggtttcg
    47581 tcggagttag cggagatagt accaacctga gcaatcgctt tagagtcaga gcacggtacg
    47641 gacagcgctt tcagttcttc aactgcagcg gtaacagctt tgtcgatacc acgtttcagg
    47701 tccatcgggt tcatgcccgc agcaacagct ttcagacctt cagtgatgat agcctgagcc
    47761 agtacggttg cagtggtggt accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct
    47821 ttcaccatct gcgcacccat gttttcgaac ttgtcttcca gttcgatttc acgagcaacg
    47881 gaaacaccat ctttggtgat ggtcggtgca ccgaaagatt tatccagaac tacgttacgg
    47941 cctttcggac cgagggtaac tttcactgca tctgccagta cgtttacgcc gcgcagcatt
    48001 ttcacacgag cgtcgttacc gaattttacg tctttagctg ccattatctt tattccttaa
    48061 attcgtatgt tcagtgtcgt gcgaggatta cgcttcaaca attgccagaa tgtcgctttc
    48121 ggacatgatc aacacttctt cattgtcgat cttctcggat ttcacaccgt agccatcgtt
    48181 gaaaataacg atgtcgccaa ctttcacatc cagcggcttc acttcgccat tttcaaggat
    48241 acggccattg ccgacagcca gcacttcgcc gcgggtggat ttagccgctg cagagccggt
    48301 cagaacgatg ccgccagcag atttagtttc aacttcttta cgcttgacga tcacgcgatc
    48361 atgcaatgga cgaatattca ttgataactc tcctttgaga aagtccgtat ctgttatggg
    48421 tgacgccgga gcttacgtgg tttcccggct ggtgaccaga gaaatgggga tgaggcttcg
    48481 ccccttcaag ggggaaaaag aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct
    48541 gattcacctc acatttcatc gcaatttctt catcgccgtc ataagcgaat ctgattgtgc
    48601 taccatcgaa aatctacgca gttgcccaaa atttgggcgc aatcggacat caacccatga
    48661 gtggactcaa acaagaactg gggctggccc agggcattgg cctgctatcg acgtcattat
    48721 taggcactgg cgtgtttgcc gttcctgcgt tagctgcact ggtagcgggc aataacagcc
    48781 tgtgggcgtg gcccattttg attatcttag tgttcccgat tgcgattgtg tttgcgattc
    48841 tgggtcgcca ctatcccagc gcaggcggcg tcgcgcactt cgtcggtatg gcgtttggtt
    48901 cgcggcttga gcgagtcacc ggctggctgt ttttatcggt cattcccgtg ggtttgcctg
    48961 ccgcgctaca aattgctgcc ggattcggcc aggcaatgtt tggctggcat agctggcaac
    49021 tgttgttggc agaactcggt acgctggcgt tggtgtggta tatcggtact cgcggtgcca
    49081 gttccagtgc aaatctacaa acagttattg ccggacttat cgtcgcactg attgtcgcta
    49141 tctggtgggc gggcgatatc aaacctgcga gtatcccctt ccccgcgcca ggaaatatcg
    49201 aacttaccgg gttatttgct gcgttatcag tgatgttctg gtgttttgtc ggtctggaag
    49261 catttgccca tcttgcctcg gaatttaaaa atccagaacg tgattttcct cgtgctttga
    49321 tgattggtct gctgctggca ggattagtct actggggctg tacggtagtc gtcttacact
    49381 tcgacgccta tggtgaacaa atggccgcgg cagcatcgct tccaaaaatt gtagtacaac
    49441 tgttcggtgt aggagcgtta tggattgcct gcgtaattgg ctatctggcc tgctttgcca
    49501 gtctcaacat ttatatacag agcttcgccc gcctggtctg gtcgcaggcg caacataatc
    49561 ctgaccacta cctggcacgc ctctcttctc gccatatccc gaataatgcc ctcaatgcgg
    49621 tgctcggctg ctgcgtggtg agcacgttgg tgattcatgc tttagagatc aatctggacg
    49681 ctcttattat ttatgccaat ggcatcttta ttatgattta tctgttatgc atgctggcag
    49741 gctgtaaatt attgcaaggt cgttatcgac tactggcggt ggttggcggg ctgttatgcg
    49801 ttctgttact ggcaatggtc ggctggaaaa gtctctatgc gctgatcatg ctggcggggt
    49861 tatggctgtt tctgccaaaa cgaaaaacgc cggaaaatgg cataaccaca taatccggcg
    49921 tttcgacatt aatcctggcg atcgtcttta tgatcaaggc ggtcgcggtc atcatccttt
    49981 cgctggtact caccatcaaa agtattaccg ccaccggtcc cggcgctaaa accgccgcct
    50041 ggcatgcgag aaaagcgcaa atgcggcatc aacttcactg tcagatgctt ttgcaccggc
    50101 ggcaataaaa gcagaagacc gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa
    50161 ccggcaatga tcagcgaaac acttttaatc atctccgccg ctgggttttc acccgccgcc
    50221 attttttgct gcatcagcac gaaattctta aagccctggt tacgtaccag tgacataccg
    50281 ataactgacg taaatataac cagcacgaga gtcagcaata cccccaatac atgggcaacc
    50341 tgaataaaga ttgaaatctc aatatagaca taaaggaaaa tggcaataaa aggtaaccag
    50401 cgcaaaggtt tctcctgtaa tagcagccgg ttaaacccgg ctacctgaat gggttgcgaa
    50461 tcgcgttcag cttatattgt ggtcattagc aaaatttcaa gatatttgcg caactatttt
    50521 tggtagtaat cccaaagcgg tgatctattt cacaaattaa taattaaggg gtaaaaaccg
    50581 acacttaaag tgatccagat tacggtagaa atcctcaagc agcatatgat ctcggatatt
    50641 cggtcgatgc aggggataat cgtcggtcga aaaacattcg aaaccacata tattctgtgt
    50701 gtttaaagca aatcattggc agcttgaaaa agaaggttca catgtcaaac aacattcgta
    50761 tcgaagaaga tctgttgggt accagggaag ttccagctga tgcctactat ggtgttcaca
    50821 ctctgagagc gattgaaaac ttctatatca gcaacaacaa aatcagtgat attcctgaat
    50881 ttgttcgcgg tatggtaatg gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa
    50941 ccattcctaa aagtgtagcg aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg
    51001 gaaaatgcat ggatcagttc ccggtagacg tctaccaggg cggcgcaggt acttccgtaa
    51061 acatgaacac caacgaagtg ctggccaata tcggtctgga actgatgggt caccagaaag
    51121 gtgaatatca gtacctgaac ccgaacgacc atgttaacaa atgtcagtcc actaacgacg
    51181 cctacccgac cggtttccgt atcgcagttt actcttccct gattaagctg gtagatgcga
    51241 ttaaccaatt gcgtgaaggc tttgaacgta aagcggtcga attccaggac atcctgaaaa
    51301 tgggtcgtac ccagctgcag gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt
    51361 tcagcatcct gctgaaagaa gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg
    51421 aagttaacct tggcgcaaca gcaatcggta ctggtctgaa cacgccgaaa gagtactctc
    51481 cgctggcagt gaaaaaactg gctgaagtta ctggcttccc atgcgtaccg gctgaagacc
    51541 tgatcgaagc gacctctgac tgcggcgctt atgttatggt tcacggagcg ctgaaacgcc
    51601 tggctgtgaa gatgtccaaa atctgtaacg acctgcgctt gctctcttct ggtccacgtg
    51661 ccggcctgaa cgagatcaac ctgccggaac tgcaggcggg ctcttccatc atgccagcta
    51721 aagtaaaccc ggttgttccg gaagtggtta accaggtatg cttcaaagtc atcggtaacg
    51781 acaccactgt taccatggca gcagaagcag gtcagctgca gttgaacgtt atggagccgg
    51841 tcattggcca ggctatgttc gaatccgttc acattctgac caacgcttgc tacaacctgc
    51901 tggaaaaatg cattaacggc atcactgcta acaaagaagt gtgcgaaggt tacgtttaca
    51961 actctatcgg tatcgttact tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg
    52021 tgggtaaaat ctgtgccgaa accggtaaga gtgtacgtga agtcgttctg gaacgcggtc
    52081 tgttgactga agcggaactt gacgatattt tctccgtaca gaatctgatg cacccggctt
    52141 acaaagcaaa acgctatact gatgaaagcg aacagtaatc gtacagggta gtacaaataa
    52201 aaaaggcacg tcagatgacg tgcctttttt cttgtgagca gtaacttaaa aataacaatc
    52261 taatatcaac ttgttaaaaa acaaggaagg ctaatatgct agttgtagaa ctcatcatag
    52321 ttttgctggc gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg
    52381 gattgggggt gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg
    52441 tcatttccat tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc
    52501 tggactatct ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga
    52561 tcctcgcacc gatcgtgacc tatttcctga ctatctttgc tggtactggc aacatctctc
    52621 tggcgacact gccagttatc gctgaagttg cgaaggaaca aggcgttaaa ccttgccgtc
    52681 cgctgtctac tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg
    52741 cagtggttta catgtcttcc gtgatggaag gtcatggcat cagctacctc catctgctct
    52801 ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat gtccttcctg gtcactatgc
    52861 tgttcaactc caaactctct gacgatccga tttatcgcaa gcgtctggaa gagggcctgg
    52921 ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc
    52981 tgttcctgct gggcgtagtt ggcgtggtta tctatgcaat catcaacagc ccaagcatgg
    53041 gtctggttga aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagtg
    53101 ttgcaactct gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca
    53161 ccttcaaagc aggtatgagc gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata
    53221 ctttcgtttc caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc
    53281 atccgtggct gctggccgtc atcttcttct ttgcttctgc tctgctgtac tctcaggctg
    53341 caaccgcaaa agcactgatg ccgatggctc tggcactgaa cgtttctccg ctgaccgctg
    53401 ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg
    53461 ctgcggtaca gatggatgac acgggtacta cccgtatcgg taaattcgtc ttcaaccatc
    53521 cgttcttcat cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg
    53581 gtagcttcat gctgtaatga ccaatcgcgg ggcgttcacg ccccgctttc tttctcgccg
    53641 actaacatcc tttccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct
    53701 tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt
    53761 accgcaccgg atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg
    53821 gcggcctgcg cgaccttgat ccccggcgct acctctctct attactggga aggtaagctg
    53881 gagcaagaat acgaagtgca gatgatttta aaaactaccg tatctcacca gcaggcactg
    53941 ctggaatgcc tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt
    54001 acacacggag acacagatta cctctcatgg ctcaacgcat ctttacgctg atcctgctac
    54061 tttgcagcac ttccgttttt gccggattat tcgacgcgcc gggacgttca caatttgtcc
    54121 ccgtggatca agcctttgct tttgattttc agcaaaacca acatgacctt aatctgacct
    54181 ggcagatcaa agacggttac tacctctacc gtaaacagat ccgcattacg ccggaacacg
    54241 cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca tgaagatgag ttttacggca
    54301 aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg
    54361 gagcgacgtt aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag
    54421 aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt
    54481 ctgttccgca gcaagagcag cccaccgcgc aattgccctt ttccgcgctc tgggcgttgt
    54541 tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg
    54601 gcatcgtgct gggtggtaaa cagcggctct ccactgccag agcattgttg ctgaccttta
    54661 tttatgtgca ggggatggca ctgacttaca cggcgctggg tctggtagtt gccgccgcag
    54721 ggttacagtt ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct
    54781 ttaccttgct ggcgatgtca atgtttggct tgcttactct acaactcccc tcctcgctgc
    54841 aaacgcgcct cacgctgatg agcaatcgcc aacagggcgg ctcacccggc ggagtgttta
    54901 ttatggggac gattgccgga ctgatctgtt caccatgcac caccgcaccg cttagcgcga
    54961 ttctgctgta tatcgcccaa agcgggaaca tgtggctggg cggcggcacg ctttatctct
    55021 atgcgttggg catgggcctg ccgctgatgc taattaccgt ctttggtaac cgcttgctgc
    55081 cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac
    55141 tgccggtctt cctgctggag cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg
    55201 cgctgggtgt cgcattcttt ggctgggcct ttatcaccag cctacaggcc aaacgcggct
    55261 ggatgcgcgt ggtgcaaata atcctgcttg cagcggcatt ggttagcgtg cgcccacttc
    55321 aggattgggc atttggtgcg acgcataccg cgcaaactca gacgcatctc aactttacac
    55381 aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccggtga
    55441 tgttagatct ttatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca
    55501 gcgacccgca ggtgcaaaaa gcgttagcag acacggtatt acttcaggcc aacgtcactg
    55561 ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa
    55621 ttctcttttt tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg
    55681 atgctgaaac cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg
    55741 gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat
    55801 tagaattaca agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc
    55861 cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc
    55921 gttatcttag ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa
    55981 ccgccgaaca aaagctgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc
    56041 gctatccggg ctgtctgttt atcgctgcct gtacgtttta tcccgatcct ggccacccta
    56101 ttcatcaact ggccgatcag caaaaaagcg cggcctacga tttcacccac gaactgttaa
    56161 ccacgctgga agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag
    56221 gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga tgtcgacacc gcacatcggc
    56281 tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg tggtgcactg acctgacaga
    56341 aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt
    56401 aggttgacga gatgtgcaga ttacggttta atgcgccccg ttgcccggat agctcagtcg
    56461 gtagagcagg ggattgaaaa tccccgtgtc cttggttcaa ttccgagtcc gggcaccaaa
    56521 ttcatataaa cggacctcca cggaggtccg tttttcgttt caggacgcca cgatttaagt
    56581 gttctgcctc caaatcaatt ctaccgaact caacccgatt ctccccacat caccagcaat
    56641 ttgcgggcat atcccaattc gggaaaattt gttcctgagc tatagcacta actaacgtga
    56701 aatgtcgtgc ggccccgtga tgctgttgaa cgtcaaatga cgtcatcagg agcgtaacgc
    56761 atccataaag cgcaacatcg gtctgaacgc cgactgatga gattttctga atgagaacaa
    56821 agagaaatgt atcagtccgc ttgctcatgc aaagactaac aatccattaa aatagtaagc
    56881 gctccggaca attttccatg gattattttc tgaacatttt tctttggcaa agatgatgaa
    56941 ttttgatggt aaggaatatt acttctggtt ctcagtaaaa tcctttcgta ataccatgta
    57001 atcaagaagt ttatggctgg taaaaataac gtcttgcatt caccaataat atgtaaataa
    57061 acccatctat agatggtaaa aataggttgt ggcaattatc attgcatcat tcccttttcg
    57121 aatgagtttc tattatgcaa caacctgtag ttcgcgttgg cgaatggctt gttactccgt
    57181 ccataaacca aattagccgc aatgggcgtc aacttaccct tgagccgaga ttaatcgacc
    57241 ttctggtttt ctttgctcaa cacagtggcg aagtacttag cagggatgaa cttatcgata
    57301 atgtctggaa gagaagtatt gtcaccaatc acgttgtgac gcagagtatc tcagaactac
    57361 gtaagtcatt aaaagataat gatgaagata gtcctgtcta tatcgctact gtaccaaagc
    57421 gcggctataa attaatggtg ccggttatct ggtacagcga agaagaggga gaggaaataa
    57481 tgctatcttc gcctccccct ataccagagg cggttcctgc cacagattct ccctcccaca
    57541 gtcttaacat tcaaaacacc gcaacgccac ctgaacaatc cccagttaaa agcaaacgat
    57601 tcactacctt ttgggtatgg ttttttttcc tgttgtcctt aggtatctgt gtagcactgg
    57661 tagcgttttc aagtcttgat acacgtcttc cgatgagcaa atcgcgtatt ttgctcaatc
    57721 cacgcgatat tgacattaat atggtaaata aaagttgtaa cagctggagt tccccgtatc
    57781 agctctctta cgcgataggc gtgggtgatt tggtggcgac atcacttaac accttctcca
    57841 cctttatggt gcatgacaaa atcaactaca acattgatga accgagcagt tccggtaaaa
    57901 cattatctat tgcgtttgtt aatcagcgcc aataccgtgc tcaacaatgc tttatgtcga
    57961 taaaattggt agacaatgca gatggttcaa ccatgctgga taaacgttat gtcatcacta
    58021 acggtaatca gctggcgatt caaaatgatt tactggagag tttatcaaaa gcgttaaacc
    58081 aaccgtggcc acaacgaatg caggagacgc tccagcaaat tttgcctcat cgtggtgcgt
    58141 tattaactaa tttttatcag gcacatgatt atttactgca tggcgatgat aaatcattga
    58201 accgtgccag tgaattatta ggtgagattg ttcaatcatc cccagaattt acctacgcga
    58261 gagcagaaaa agcattagtt gatatcgtgc gccattctca acatccttta gatgaaaaac
    58321 aattagcagc actgaacaca gaaatagata acattgttac actaccggaa ttgaataacc
    58381 tgtccattat atatcaaata aaagcggtca gtgctctggt aaaaggtaaa acagatgagt
    58441 cttaccaggc gataaatact ggcattgatc ttgaaatgtc ctggctaaat tatgtattgc
    58501 ttggcaaggt ttatgaaatg aaggggatga accgggaagc ggctgatgca tatctcaccg
    58561 cctttaattt acgcccaggg gcaaacaccc tttactggat tgaaaatggt atattccaga
    58621 cttctgttcc ttatgttgta ccttatctcg acaaatttct cgcttcagaa taagtaaccc
    58681 ccgggttgat tcatgctcgg gaatatttgt tgttgagttt ttgtatgttc ctgttggtat
    58741 aatatgttgc ggcaatttat ttgccgcata atttttatta cataaattta accagaagac
    58801 gtcacgcaat ctattgtaaa cattgaatgt ttatcttttc atgatatcaa cttgcgatcc
    58861 tgatgtgtta ataaaaaacc tcaagttctc acttacagaa acttttgtgt tatttcacct
    58921 aatctttagg attaatcctt ttttcgtgag taatcttagc gccagtttgg tctggtcagg
    58981 aaatagttat acatcatgac ccggactcca aattcaaaaa tgaaattagg agaagagcat
    59041 gagttctgcc aagaagatcg ggctatttgc ctgtaccggt gttgttgccg gtaatatgat
    59101 ggggagcggt attgcattat tacctgcgaa cctagcaagt atcggtggta ttgctatctg
    59161 gggttggatt atctctatta ttggtgcaat gtcgctggcg tatgtatatg cccgactggc
    59221 aacaaaaaac ccgcaacaag gtggcccaat tgcttatgcc ggagaaattt ctcctgcatt
    59281 tggttttcag acaggtgttc tttattacca tgctaactgg attggtaacc tggcgattgg
    59341 tattaccgct gtatcttatc tttccacctt cttcccagta ttaaatgatc ctgttccggc
    59401 gggtatcgcc tgtattgcta tcgtctgggt atttaccttt gtaaatatgc tcggcggtac
    59461 ctgggtaagc cgtttaacca ctattggtct ggtgctggtt cttattcctg tggtgatgac
    59521 tgctattgtt ggctggcatt ggtttgatgc ggcaacttat gcagctaact ggaatactgc
    59581 ggataccact gatggtcatg cgatcattaa aagtattctg ctctgcctgt gggccttcgt
    59641 gggtgttgaa tccgcagctg taagtactgg tatggttaaa aacccgaaac gtaccgttcc
    59701 gctggcaacc atgctgggta ctggtttagc aggtattgtt tacatcgctg cgactcaggt
    59761 gctttccggt atgtatccgt cttctgtaat ggcggcttcc ggtgctccgt ttgcaatcag
    59821 tgcttcaact atcctcggta actgggctgc gccgctggtt tctgcattca ccgcctttgc
    59881 gtgcctgact tctctgggct cctggatgat gttggtaggc caggcaggtg tacgtgccgc
    59941 taacgacggt aacttcccga aagtttatgg tgaagtcgac agcaacggta ttccgaaaaa
    60001 aggtctgctg ctggctgcag tgaaaatgac tgccctgatg atcctcatca ctctgatgaa
    60061 ctctgccggt ggtaaagcat ctgacctgtt cggtgaactg accggtatcg cagtactgct
    60121 gactatgctg ccgtacttct actcttgcgt tgacctgatt cgttttgaag gcgttaacat
    60181 ccgcaacttt gtcagcctga tctgctctgt actgggttgc gtgttctgct tcatcgcgct
    60241 gatgggcgca agctccttcg agctggcagg taccttcatc gtcagcctga ttatcctgat
    60301 gttctacgct cgcaaaatgc acgagcgcca gagccactca atggataacc acaccgcgtc
    60361 taacgcacat taattaaaag tattttccga ggctcctcct ttcattttgt cccatgtgtt
    60421 gggaggggcc ttttttacct ggagatatga ctatgaacgt tattgcaata ttgaatcaca
    60481 tgggggttta ttttaaagaa gaacccatcc gtgaacttca tcgcgcgctt gaacgtctga
    60541 acttccagat tgtttacccg aacgaccgtg acgacttatt aaaactgatc gaaaacaatg
    60601 cgcgtctgtg cggcgttatt tttgactggg ataaatataa tctcgagctg tgcgaagaaa
    60661 ttagcaaaat gaacgagaac ctgccgttgt acgcgttcgc taatacgtat tccactctcg
    60721 atgtaagcct gaatgacctg cgtttacaga ttagcttctt tgaatatgcg ctgggtgctg
    60781 ctgaagatat tgctaacaag atcaagcaga ccactgacga atatatcaac actattctgc
    60841 ctccgctgac taaagcactg tttaaatatg ttcgtgaagg taaatatact ttctgtactc
    60901 ctggtcacat gggtggtact gcattccaga aaagcccggt aggtagcctg ttctatgatt
    60961 tctttggtcc gaataccatg aaatctgata tttccatttc agtatctgaa ctgggttctc
    61021 tgctggatca cagtggtcca cacaaagaag cagaacagta tatcgctcgc gtctttaacg
    61081 cagaccgcag ctacatggtg accaacggta cttccactgc gaacaaaatt gttggtatgt
    61141 actctgctcc ggcaggcagc accattctga ttgaccgtaa ctgccacaaa tcgctgaccc
    61201 acctgatgat gatgagcgat gttacgccaa tctatttccg cccgacccgt aacgcttacg
    61261 gtattcttgg tggtatccca cagagtgaat tccagcacgc taccattgct aagcgcgtga
    61321 aagaaacacc aaacgcaacc tggccggtac atgctgtaat taccaactct acctatgatg
    61381 gtctgctgta caacaccgac ttcatcaaga aaacactgga tgtgaaatcc atccactttg
    61441 actccgcgtg ggtgccttac accaacttct caccgattta cgaaggtaaa tgcggtatga
    61501 gcggtggccg tgtagaaggg aaagtgattt acgaaaccca gtccactcac aaactgctgg
    61561 cggcgttctc tcaggcttcc atgatccacg ttaaaggtga cgtaaacgaa gaaaccttta
    61621 acgaagccta catgatgcac accaccactt ctccgcacta cggtatcgtg gcgtccactg
    61681 aaaccgctgc ggcgatgatg aaaggcaatg caggtaagcg tctgatcaac ggttccattg
    61741 aacgtgcgat caaattccgt aaagagatca aacgtctgag aacggaatct gatggctggt
    61801 tctttgatgt atggcagccg gatcatatcg atacgactga atgctggccg ctgcgttctg
    61861 acagcacctg gcacggcttc aaaaacatcg ataacgagca catgtatctt gacccgatca
    61921 aagtcaccct gctgactccg gggatggaaa aagacggcac catgagcgac tttggtattc
    61981 cggccagcat cgtggcgaaa tacctcgacg aacatggcat cgttgttgag aaaaccggtc
    62041 cgtataacct gctgttcctg ttcagcatcg gtatcgataa gaccaaagca ctgagcctgc
    62101 tgcgtgctct gactgacttc aaacgtgcgt tcgacctgaa cctgcgtgtg aaaaacatgc
    62161 tgccgtctct gtatcgtgaa gatcctgaat tctatgaaaa catgcgtatt caggaactgg
    62221 ctcagaatat ccacaaactg attgttcacc acaatctgcc ggatctgatg tatcgcgcat
    62281 ttgaagtgct gccgacgatg gtaatgactc cgtatgctgc attccagaaa gagctgcacg
    62341 gtatgaccga agaagtttac ctcgacgaaa tggtcggtcg tattaacgcc aatatgatcc
    62401 ttccgtatcc gccgggagtt cctctggtaa tgccgggtga aatgatcacc gaagaaagcc
    62461 gtccggttct ggagttcctg cagatgctgt gtgaaatcgg cgctcactat ccgggctttg
    62521 aaaccgatat tcacggtgca taccgtcagg ctgatggccg ctataccgtt aaggtattga
    62581 aagaagaaag caaaaaataa ttagctcgta caagggaagt ggcttgccac ttcccttttt
    62641 tgctcataag gagaacacat gaaaacaccc tcacagccgc gcgcgatata ctatatcgtg
    62701 gcgatccaaa tctgggagta cttcagtttt tacggcatgc gtgccttact cattctctat
    62761 ctcacccatc agcttggttt tgatgataac catgccatca gcctgttcag cgcatatgct
    62821 tctctggttt acgttacccc tattctcggc ggctggcttg ccgaccgcct gctcggcaac
    62881 cgcactgcag tgattgccgg cgcgctgtta atgacccttg gccatgtggt gctgggtatt
    62941 gatacaaatt caacctttag cctgtatctg gcgctggcaa tcattatttg tggctacggt
    63001 ttattcaaat caaacatcag ctgtttgctt ggcgagctct acgacgagaa cgatcatcga
    63061 cgtgatggcg gtttttcgct gctgtatgct gcgggcaata tcggttctat cgccgcccca
    63121 atcgcctgcg gcctggctgc tcagtggtat ggatggcatg ttggctttgc ccttgcgggt
    63181 ggcggcatgt ttatcggttt gttgattttc ttaagcgggc atcgtcattt ccagtccacg
    63241 cgtagtatgg ataaaaaagc gctcactagc gtcaaatttg ccttaccggt atggagctgg
    63301 ttagtggtga tgctctgttt agccccagta ttttttaccc tgctgctgga gaacgactgg
    63361 tccggatatt tgctggcgat cgtttgcctc attgccgcac aaatcattgc ccgcatgatg
    63421 atcaaattcc ccgaacaccg ccgtgctctt tggcaaattg tattgttgat gtttgtcggg
    63481 acattgttct gggtactggc acaacagggc ggcagtacca tcagcttgtt tatcgatcgc
    63541 tttgtgaatc gtcaggcatt caatattgaa gtacctactg cactattcca gtcggtgaat
    63601 gcaattgcgg tgatgctcgc tggggttgtt ctggcctggc tggcgtcgcc agaaagccac
    63661 ggcaactcaa cattgcgcgt ctggctgaag tttgcctttg gcttactgct gatggcttgt
    63721 ggctttatgt tgctggcatt tgatgcccga catgcagcag ctgacggtca agcgtcaatg
    63781 ggcgtgatgg tatccgggct ggcgctaatg ggctttgccg aactctttat tgacccggtg
    63841 gcgattgcgc aaatcacgcg tctgaaaatg tctggcgtat taaccgggat ttatatgctg
    63901 gcaacaggcg cggtcgccaa ctggctggca ggcgtagtgg cacagcagac gacagagtcg
    63961 caaattagcg gtatggcaat tgcagcttac cagcgattct tttctcagat gggagagtgg
    64021 acgttggctt gtgtcgcgat catcgtggta ttggcctttg ctacccgttt cctgtttagc
    64081 acgccgacga atatggtaca ggagagcaac gattaactgt cgtaaaaaca attggttatg
    64141 aataaattaa ctttcagttt atcacactag taaatcgaga ttaataagtc atttaaacca
    64201 ttttgatggt tatttattag tgatatcaac ttgaggtaag cgttagtttc gataagataa
    64261 actgagttat taatagtcga ggcagataat acagtgtacc gatctgtctc tttatctacg
    64321 ctaaattgaa agctggattt agaggaacca aaatgtctga acaagaaaca cggggagcca
    64381 atgaggctat tgattttaac gatgaactga gaaatcgccg cgaaaaactg gcggcactac
    64441 gtcagcaagg tgtggcgttt cccaatgatt ttcgccgcga ccatacctct gaccagttgc
    64501 acgaagagtt tgatgcgaag gataaccagg aactggaatc cttaaacatt gaagtctcgg
    64561 ttgctggccg aatgatgacc cgtcgtatca tggggaaagc ctcctttgta acgttgcagg
    64621 atgtcggtgg ccgtattcaa ctgtacgtcg cacgagatag cctgccagaa ggtgtttata
    64681 acgatcagtt taaaaaatgg gatctgggtg acattatcgg tgcccgcggt acgctgttta
    64741 agacgcaaac gggtgagctt tccattcact gtactgagtt gcgcctgctg actaaagcac
    64801 tacgtccttt accagataaa ttccatggtc tgcaggatca ggaagtccgt tatcgtcaac
    64861 gttatctgga cctcatcgct aacgataaat cccgtcaaac gtttgttgtc cgttcaaaaa
    64921 ttctggccgc tatccgccag ttcatggtcg cgcgcggctt tatggaagta gaaaccccga
    64981 tgatgcaggt aattccaggt ggggcatctg ctcgcccgtt tattacccat cataatgctc
    65041 tggatttaga tatgtatctg cgtatcgcgc cggagctgta tctgaaacgt ctggttgtag
    65101 gcggttttga acgggtattc gaaatcaacc gtaacttccg taatgaaggt atttctgttc
    65161 gccataatcc tgagttcaca atgatggaac tctacatggc gtatgcggat taccacgatt
    65221 tgattgaact gacagagtcc ctgttccgca ccctggcaca agaggttctg ggtaccacta
    65281 aagtcactta tggcgagcat gtgtttgatt tcggcaaacc gtttgaaaaa ctcaccatgc
    65341 gcgaagcaat caaaaaatat cgtccagaaa ccgatatggc cgacctggat aattttgatg
    65401 ctgctaaagc attagctgaa tctatcggta ttacggtaga gaaaagctgg gggctgggac
    65461 gtattgtcac ggagatcttt gatgaagtgg cagaagcaca tctgattcag ccaaccttta
    65521 ttacggaata tccggcagaa gtgtcaccgc tggcacgccg taatgatgtt aacccggaaa
    65581 tcaccgaccg ttttgaattc ttcatcggtg gtcgtgaaat cggtaatggt tttagcgaat
    65641 taaacgacgc agaagatcag gctgaacgtt tccaggaaca ggttaacgct aaagccgcag
    65701 gtgacgacga agccatgttc tatgacgaag attacgtgac tgcgctggaa tatggtctgc
    65761 cgccaaccgc tggtctgggt attggtatcg accgaatgat tatgctgttt actaacagcc
    65821 atactattcg cgacgttatt ctcttcccgg cgatgcgccc acagaaataa atttcacttt
    65881 aatgaacgaa gcagtcaggc gactgcttcg ttcatcgttc cgcaaatcca gttttgagga
    65941 gagacgcttc gtttcacgat aaccagcggg tccactcaac tacagctcct aaaagagaga
    66001 aaaaagtaat gccacaggca gactcattgg ccaggttatt ccgaccagga atgcactaag
    66061 taaacgaata tgtgttttct catgtgagat aaaccagata atgacaaagg atatgttcac
    66121 accaatcaca taaaaaatta atattttaga gaatagtgcc attttttatg ttcttcttat
    66181 ccttactggc tacttgtaaa actgacggaa atagtcatca actgtcataa tgttgaggtc
    66241 aaagtcaata tccggtaagt ctaagcattt aagcctgatt aaatcttcga tttcacttgc
    66301 ggctaacgat gtcgttattg cgaaggaatt ttcattcaat tgtcgtggta tcccatattc
    66361 atcagccacg atagaagaga aattcatatc atacatcaat gatcttaacc ttaagaacac
    66421 tgaggaataa gatgatggat aatcaaaaga aacaacataa gtattgaact tgtttttacc
    66481 ttccataata tgactcctat aaatattgaa cactctgggg acgcaaaatt tattaacccc
    66541 gattgatgat tctcatatag gagatattct tttttttgct cagtaaaggg gatgacttgg
    66601 gacaaaaaaa cgcgctctct gcccattctt tgtgcatccc cctacccttc agatgctctc
    66661 ctgcatcaca ccgcggagag catgaaaaat atcgtgaatc tgtactcttc catcaactgc
    66721 gaatatcatc atactcccgc gttttatcaa attcgtgttt cgcaaatgga cataatggga
    66781 taatttttcg tttttcccga cgcatttttt ccacgacttt cgcaaccagc tgtttaccaa
    66841 tcccttgccc tttcaggctt tcatcgacat cggtatgttc gataatcgct aaattctctc
    66901 cggtcggcac aaagacaatt tcagcgattt gcttgccttg ttcgtcatta atgtaaaatt
    66961 tattgtggcc ttcgcgtatt tccattttac cctcgcttat ttatgacggt atttcagcgc
    67021 accactcggg cacgtatcaa tcactttaac cacagtggca acgtcgactt catccggcat
    67081 gatccacggc tttcgtttga gattaaataa cttgccgttg ccacgtacgc aattgccaga
    67141 atgctgacat atcgcagtgt tgaaatagac atcgatcttt tcgccggtat aacagcgata
    67201 acccccgtcc agtagcgcct gatccatgac cttccctctc tcatcagtat ggttttcact
    67261 ataagcatag ccctggaatc ttcatacagc actggcagtt tccgttgcca gagtctcttc
    67321 ccctatatag ccaatcattc ttactccctt tgaattaccc gcctcatcag agataatgct
    67381 taagaaattt gtcacacaag gaagctgatg agacattcat tgccctaccg catgttacgc
    67441 aaacgtccga tgaaattgag taccacagtg atcttaatgg tcagcgcggt actgttctcg
    67501 gtgctattgg tggtgcatct gatttacttc tcgcaaatca gtgatatgac gcgagacggg
    67561 ctagccaaca aggcactggc agtggcgcgt actctcgccg actcgccgga aatccgtcag
    67621 ggcttgcaga aaaaaccgca ggagagcggc atccaggcca tcgcggaagc cgtgcgcaaa
    67681 cgcaacgatc tgctgtttat tgtcgttacc gatatgcaaa gtcttcgcta ctcgcatcct
    67741 gaagcccagc gtattggtca gccatttaaa ggtgatgaca tccttaaagc gctgaatggc
    67801 gaagaaaatg tcgctatcaa tcgcggtttt ctggcgcagg ctttacgcgt atttaccccc
    67861 atctacgatg aaaatcataa acaaattggc gtggtggcga tcggccttga gttaagccgc
    67921 gtgacccaac agatcaatga cagtcgctgg agcattatct ggtcggtatt atttggcatg
    67981 ctggtcggac tgattggcac ctgcattctg gttaaggtac tgaaaaaaat ccttttcggc
    68041 ctggaaccct acgaaatctc cacgctgttt gagcaacgcc aggccatgtt gcagtctatc
    68101 aaagaaggcg tcgttgccgt ggacgatcgc ggcgaggtca cgctgatcaa cgatgccgca
    68161 caagaattgc tgaattaccg taagtcgcag gacgatgaga aactgtcgac gctcagccac
    68221 tcatggtcac aggtggtaga tgtctcggaa gtgttacgcg acggtacgcc gcgccgcgac
    68281 gaagagatta cgattaaaga ccggctatta ctgatcaaca ccgttccggt gcgcagtaat
    68341 ggcgttatca tcggtgccat ttcaaccttc agggacaaaa ctgaagttcg taaactgatg
    68401 cagcgactcg acggtctggt caactatgct gatgcacttc gtgaacgatc ccacgaattt
    68461 atgaataaat tgcatgtgat tctcggatta ttgcatctga agagttataa gcagttggaa
    68521 gattacattc tcaaaacagc caataactat caggaagaga ttggctctct gctgggtaag
    68581 atcaaatctc cggttatcgc tggtttttta atcagcaaga ttaaccgcgc gaccgattta
    68641 ggccatacgc tgattttaaa cagtgaaagc cagctgccag acagcggcag tgaggaccag
    68701 gtcgcgacgc tgattaccac attgggaaat ctgatagaaa acgcgctgga agcattaggg
    68761 ccggaacccg gtggcgaaat tagcgtaaca ttgcactacc gtcacggctg gctgcactgc
    68821 gaagttaacg atgatggacc ggggatcgca cccgacaaaa tcgatcacat ttttgacaaa
    68881 ggtgtctcga caaaaggaag cgagcgaggc gtcggtttag cacttgtcaa acaacaggta
    68941 gaaaatctcg gcggcagcat cgccgtggaa tcggaacccg ggattttcac acaatttttt
    69001 gtccagatac cctgggacgg ggagaggtcg aacagatgat caatgtatta attatcgatg
    69061 acgacgcaat ggtcgcggag ctgaatcgcc gatacgtagc acaaatccca ggctttcaat
    69121 gctgtggaac agcctcgacg ctggagaaag ccaaagagat tatcttcaat agcgatacgc
    69181 ctatcgacct gatattgctc gatatctata tgcaaaaaga gaacgggctc gatttactgc
    69241 ctgtcctgca taacgcgcgt tgcaaaagtg atgtgattgt catctcctcc gcagccgatg
    69301 cggcaaccat taaagattcg ctgcattacg gtgtcgtgga ttacctgatc aaacccttcc
    69361 aggcttcccg ctttgaagag gcgctcaccg gctggcggca aaagaaaatg gcgctggaaa
    69421 aacatcagta ttatgaccag gccgagcttg atcagctaat ccacggcagc agctccaacg
    69481 agcaggatcc tcgtcgcttg ccaaaaggct taacgccgca gacgttacgc acactgtgcc
    69541 agtggattga cgcgcatcag gactatgagt tttcaaccga cgagctagcc aacgaggtta
    69601 acatttcgcg tgtttcctgc cgtaaatacc ttatctggct ggtcaactgc cacatcttgt
    69661 tcaccagtat ccattatggc gtcacggggc gtccagttta ccgttaccgt attcaggcag
    69721 aacactactc actactgaaa caatattgcc aataacggat cgctggttat ctgtaagtaa
    69781 taattattac cgtaatgcaa aatacccctg gtacaagtta catccttcat cagtaaaact
    69841 tgtgccagat caaatataat tatccctcca tcatcgctaa aaattaatat ctcttcaggt
    69901 gaacggtgtt tttaatttca aaacgctaac aaaagttaat taactattat gtca
//
LOCUS       DAZGXS010000009        65326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGXS010000009 DAZGXS010000000
VERSION     DAZGXS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            77..709
                     /gene="comR"
                     /locus_tag="ACHKG5_000702"
     CDS             77..709
                     /gene="comR"
                     /locus_tag="ACHKG5_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0866867.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(771..1310)
                     /gene="ycfJ"
                     /locus_tag="ACHKG5_000703"
     CDS             complement(771..1310)
                     /gene="ycfJ"
                     /locus_tag="ACHKG5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0866868.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1537..2841)
                     /gene="ndh"
                     /locus_tag="ACHKG5_000704"
     CDS             complement(1537..2841)
                     /gene="ndh"
                     /locus_tag="ACHKG5_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0866869.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3241..3783)
                     /gene="ycfP"
                     /locus_tag="ACHKG5_000705"
     CDS             complement(3241..3783)
                     /gene="ycfP"
                     /locus_tag="ACHKG5_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0866870.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3806..4831)
                     /gene="nagZ"
                     /locus_tag="ACHKG5_000706"
     CDS             complement(3806..4831)
                     /gene="nagZ"
                     /locus_tag="ACHKG5_000706"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0866871.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4842..5666)
                     /gene="thiK"
                     /locus_tag="ACHKG5_000707"
     CDS             complement(4842..5666)
                     /gene="thiK"
                     /locus_tag="ACHKG5_000707"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0866872.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5647..6288)
                     /gene="lpoB"
                     /locus_tag="ACHKG5_000708"
     CDS             complement(5647..6288)
                     /gene="lpoB"
                     /locus_tag="ACHKG5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0866873.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6302..6679)
                     /gene="ycfL"
                     /locus_tag="ACHKG5_000709"
     CDS             complement(6302..6679)
                     /gene="ycfL"
                     /locus_tag="ACHKG5_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0866874.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6682..7041)
                     /gene="hinT"
                     /locus_tag="ACHKG5_000710"
     CDS             complement(6682..7041)
                     /gene="hinT"
                     /locus_tag="ACHKG5_000710"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0866875.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7172..7354
                     /locus_tag="ACHKG5_000711"
     CDS             7172..7354
                     /locus_tag="ACHKG5_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866876.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECAYFSFAFAFAFAS
                     KIIRTLNITAHHPPPA"
     gene            7387..9576
                     /gene="fhuE"
                     /locus_tag="ACHKG5_000712"
     CDS             7387..9576
                     /gene="fhuE"
                     /locus_tag="ACHKG5_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP0866877.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9636..11069)
                     /gene="ptsG"
                     /locus_tag="ACHKG5_000713"
     CDS             complement(9636..11069)
                     /gene="ptsG"
                     /locus_tag="ACHKG5_000713"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0866878.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11364..12161)
                     /gene="ycfH"
                     /locus_tag="ACHKG5_000714"
     CDS             complement(11364..12161)
                     /gene="ycfH"
                     /locus_tag="ACHKG5_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0866879.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12172..13176)
                     /gene="holB"
                     /locus_tag="ACHKG5_000715"
     CDS             complement(12172..13176)
                     /gene="holB"
                     /locus_tag="ACHKG5_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0866880.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIRERLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13173..13814)
                     /gene="tmk"
                     /locus_tag="ACHKG5_000716"
     CDS             complement(13173..13814)
                     /gene="tmk"
                     /locus_tag="ACHKG5_000716"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0866881.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13804..14826)
                     /gene="yceG"
                     /locus_tag="ACHKG5_000717"
     CDS             complement(13804..14826)
                     /gene="yceG"
                     /locus_tag="ACHKG5_000717"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0866882.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14829..15638)
                     /gene="pabC"
                     /locus_tag="ACHKG5_000718"
     CDS             complement(14829..15638)
                     /gene="pabC"
                     /locus_tag="ACHKG5_000718"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0866883.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15758..16999)
                     /gene="fabF"
                     /locus_tag="ACHKG5_000719"
     CDS             complement(15758..16999)
                     /gene="fabF"
                     /locus_tag="ACHKG5_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0866884.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17087..17323)
                     /gene="acpP"
                     /locus_tag="ACHKG5_000720"
     CDS             complement(17087..17323)
                     /gene="acpP"
                     /locus_tag="ACHKG5_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0866885.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17534..18268)
                     /gene="fabG"
                     /locus_tag="ACHKG5_000721"
     CDS             complement(17534..18268)
                     /gene="fabG"
                     /locus_tag="ACHKG5_000721"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0866886.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18281..19210)
                     /gene="fabD"
                     /locus_tag="ACHKG5_000722"
     CDS             complement(18281..19210)
                     /gene="fabD"
                     /locus_tag="ACHKG5_000722"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0866887.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19226..20179)
                     /gene="fabH"
                     /locus_tag="ACHKG5_000723"
     CDS             complement(19226..20179)
                     /gene="fabH"
                     /locus_tag="ACHKG5_000723"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0866888.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20247..21317)
                     /gene="plsX"
                     /locus_tag="ACHKG5_000724"
     CDS             complement(20247..21317)
                     /gene="plsX"
                     /locus_tag="ACHKG5_000724"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0866889.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21398..21571)
                     /gene="rpmF"
                     /locus_tag="ACHKG5_000725"
     CDS             complement(21398..21571)
                     /gene="rpmF"
                     /locus_tag="ACHKG5_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0866890.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21623..22144)
                     /gene="yceD"
                     /locus_tag="ACHKG5_000726"
     CDS             complement(21623..22144)
                     /gene="yceD"
                     /locus_tag="ACHKG5_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0866891.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22343..22927
                     /gene="yceF"
                     /locus_tag="ACHKG5_000727"
     CDS             22343..22927
                     /gene="yceF"
                     /locus_tag="ACHKG5_000727"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0866892.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23028..23987)
                     /gene="rluC"
                     /locus_tag="ACHKG5_000728"
     CDS             complement(23028..23987)
                     /gene="rluC"
                     /locus_tag="ACHKG5_000728"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0866893.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24560..27745
                     /gene="rne"
                     /locus_tag="ACHKG5_000729"
     CDS             24560..27745
                     /gene="rne"
                     /locus_tag="ACHKG5_000729"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0866894.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27940..28893)
                     /gene="flgL"
                     /locus_tag="ACHKG5_000730"
     CDS             complement(27940..28893)
                     /gene="flgL"
                     /locus_tag="ACHKG5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0866895.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28905..30548)
                     /gene="flgK"
                     /locus_tag="ACHKG5_000731"
     CDS             complement(28905..30548)
                     /gene="flgK"
                     /locus_tag="ACHKG5_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0866896.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30614..31555)
                     /gene="flgJ"
                     /locus_tag="ACHKG5_000732"
     CDS             complement(30614..31555)
                     /gene="flgJ"
                     /locus_tag="ACHKG5_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0866897.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31555..32652)
                     /gene="flgI"
                     /locus_tag="ACHKG5_000733"
     CDS             complement(31555..32652)
                     /gene="flgI"
                     /locus_tag="ACHKG5_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0866898.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32664..33362)
                     /gene="flgH"
                     /locus_tag="ACHKG5_000734"
     CDS             complement(32664..33362)
                     /gene="flgH"
                     /locus_tag="ACHKG5_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0866899.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGTT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33415..34197)
                     /gene="flgG"
                     /locus_tag="ACHKG5_000735"
     CDS             complement(33415..34197)
                     /gene="flgG"
                     /locus_tag="ACHKG5_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0866900.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34335..35090)
                     /gene="flgF"
                     /locus_tag="ACHKG5_000736"
     CDS             complement(34335..35090)
                     /gene="flgF"
                     /locus_tag="ACHKG5_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0866901.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35110..36315)
                     /gene="flgE"
                     /locus_tag="ACHKG5_000737"
     CDS             complement(35110..36315)
                     /gene="flgE"
                     /locus_tag="ACHKG5_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0866902.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36340..37035)
                     /gene="flgD"
                     /locus_tag="ACHKG5_000738"
     CDS             complement(36340..37035)
                     /gene="flgD"
                     /locus_tag="ACHKG5_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0866903.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37047..37451)
                     /gene="flgC"
                     /locus_tag="ACHKG5_000739"
     CDS             complement(37047..37451)
                     /gene="flgC"
                     /locus_tag="ACHKG5_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0866904.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37455..37871)
                     /gene="flgB"
                     /locus_tag="ACHKG5_000740"
     CDS             complement(37455..37871)
                     /gene="flgB"
                     /locus_tag="ACHKG5_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0866905.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38026..38685
                     /gene="flgA"
                     /locus_tag="ACHKG5_000741"
     CDS             38026..38685
                     /gene="flgA"
                     /locus_tag="ACHKG5_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0866906.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38761..39054
                     /gene="flgM"
                     /locus_tag="ACHKG5_000742"
     CDS             38761..39054
                     /gene="flgM"
                     /locus_tag="ACHKG5_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0866907.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39059..39475
                     /gene="flgN"
                     /locus_tag="ACHKG5_000743"
     CDS             39059..39475
                     /gene="flgN"
                     /locus_tag="ACHKG5_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0866908.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39515..41050)
                     /gene="murJ"
                     /locus_tag="ACHKG5_000744"
     CDS             complement(39515..41050)
                     /gene="murJ"
                     /locus_tag="ACHKG5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0866909.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41160..42083)
                     /gene="yceM"
                     /locus_tag="ACHKG5_000745"
     CDS             complement(41160..42083)
                     /gene="yceM"
                     /locus_tag="ACHKG5_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0866910.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42085..42732)
                     /gene="yceH"
                     /locus_tag="ACHKG5_000746"
     CDS             complement(42085..42732)
                     /gene="yceH"
                     /locus_tag="ACHKG5_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0866911.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42743..43327)
                     /gene="rimJ"
                     /locus_tag="ACHKG5_000747"
     CDS             complement(42743..43327)
                     /gene="rimJ"
                     /locus_tag="ACHKG5_000747"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0866912.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43563..44771
                     /gene="mdtH"
                     /locus_tag="ACHKG5_000748"
     CDS             43563..44771
                     /gene="mdtH"
                     /locus_tag="ACHKG5_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0866913.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44835..45482
                     /gene="grxB"
                     /locus_tag="ACHKG5_000749"
     CDS             44835..45482
                     /gene="grxB"
                     /locus_tag="ACHKG5_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0866914.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45616..46176
                     /gene="yceB"
                     /locus_tag="ACHKG5_000750"
     CDS             45616..46176
                     /gene="yceB"
                     /locus_tag="ACHKG5_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0866915.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46282..47328
                     /gene="pyrC"
                     /locus_tag="ACHKG5_000751"
     CDS             46282..47328
                     /gene="pyrC"
                     /locus_tag="ACHKG5_000751"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0866916.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47402..47647
                     /gene="dinI"
                     /locus_tag="ACHKG5_000752"
     CDS             47402..47647
                     /gene="dinI"
                     /locus_tag="ACHKG5_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0866917.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47937..48191
                     /gene="bssS"
                     /locus_tag="ACHKG5_000753"
     CDS             47937..48191
                     /gene="bssS"
                     /locus_tag="ACHKG5_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0866918.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48306..49424
                     /gene="solA"
                     /locus_tag="ACHKG5_000754"
     CDS             48306..49424
                     /gene="solA"
                     /locus_tag="ACHKG5_000754"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0866919.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            49472..49585
                     /locus_tag="ACHKG5_000755"
     CDS             49472..49585
                     /locus_tag="ACHKG5_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0866920.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49846..50412
                     /gene="yceJ"
                     /locus_tag="ACHKG5_000756"
     CDS             49846..50412
                     /gene="yceJ"
                     /locus_tag="ACHKG5_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0866921.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50416..50991
                     /gene="yceI"
                     /locus_tag="ACHKG5_000757"
     CDS             50416..50991
                     /gene="yceI"
                     /locus_tag="ACHKG5_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0866922.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51033..52085)
                     /gene="trhO"
                     /locus_tag="ACHKG5_000758"
     CDS             complement(51033..52085)
                     /gene="trhO"
                     /locus_tag="ACHKG5_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0866923.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52310..53230
                     /gene="lpxL"
                     /locus_tag="ACHKG5_000759"
     CDS             52310..53230
                     /gene="lpxL"
                     /locus_tag="ACHKG5_000759"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0866924.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53402..54628
                     /gene="mdtG"
                     /locus_tag="ACHKG5_000760"
                     /pseudo
     CDS             53402..54628
                     /gene="mdtG"
                     /locus_tag="ACHKG5_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            54711..55085
                     /gene="msyB"
                     /locus_tag="ACHKG5_000761"
     CDS             54711..55085
                     /gene="msyB"
                     /locus_tag="ACHKG5_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0866925.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55086..55313)
                     /gene="yceK"
                     /locus_tag="ACHKG5_000762"
     CDS             complement(55086..55313)
                     /gene="yceK"
                     /locus_tag="ACHKG5_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0866926.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55486..58029)
                     /gene="mdoH"
                     /locus_tag="ACHKG5_000763"
     CDS             complement(55486..58029)
                     /gene="mdoH"
                     /locus_tag="ACHKG5_000763"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0866927.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(58022..59557)
                     /gene="mdoG"
                     /locus_tag="ACHKG5_000764"
     CDS             complement(58022..59557)
                     /gene="mdoG"
                     /locus_tag="ACHKG5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0866928.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59950..61107
                     /gene="mdoC"
                     /locus_tag="ACHKG5_000765"
     CDS             59950..61107
                     /gene="mdoC"
                     /locus_tag="ACHKG5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0866929.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61115..62596)
                     /gene="clsC"
                     /locus_tag="ACHKG5_000766"
     CDS             complement(61115..62596)
                     /gene="clsC"
                     /locus_tag="ACHKG5_000766"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0866930.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62538..63071)
                     /gene="ymdB"
                     /locus_tag="ACHKG5_000767"
     CDS             complement(62538..63071)
                     /gene="ymdB"
                     /locus_tag="ACHKG5_000767"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0866931.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63166..63477)
                     /gene="ymdA"
                     /locus_tag="ACHKG5_000768"
     CDS             complement(63166..63477)
                     /gene="ymdA"
                     /locus_tag="ACHKG5_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0866932.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63598..63930)
                     /gene="csgC"
                     /locus_tag="ACHKG5_000769"
     CDS             complement(63598..63930)
                     /gene="csgC"
                     /locus_tag="ACHKG5_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0866933.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63989..64447)
                     /gene="csgA"
                     /locus_tag="ACHKG5_000770"
     CDS             complement(63989..64447)
                     /gene="csgA"
                     /locus_tag="ACHKG5_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0866934.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(64488..64943)
                     /gene="csgB"
                     /locus_tag="ACHKG5_000771"
     CDS             complement(64488..64943)
                     /gene="csgB"
                     /locus_tag="ACHKG5_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0866935.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15943 a        17345 c        16038 g        16000 t
ORIGIN      
        1 gggatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac
       61 caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg
      121 cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca
      181 cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac
      241 gctatacgcg gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat
      301 cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc
      361 tgcgctggct gactattttg ctgccatcgc caactgcttt accggcaaag acaccccggc
      421 aggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa
      481 tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg
      541 acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa
      601 ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgttgg aaaaactgat
      661 gcagattgcc ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa
      721 agcccctcag cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg
      781 ttattcaaaa tcagttgccc gttactatct agcgggatct gcgtacccgg atcgcggtcc
      841 atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt
      901 tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc
      961 tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg
     1021 gcgacatctt taccacgacc accaccaaac tgatgcccta tcacgccgcc agcaacagcg
     1081 ccgagcaccg acccggtaat gcgatgttca tcctgcaccg gtcgacgatg ggtcactgtg
     1141 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
     1201 acctgagcgt attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgcca
     1261 agcgcagctg cgacaccaat cccgataccc gccaacattg atttattcac gggaatacct
     1321 ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
     1381 atgaaaagcg cgggaatggc tggaaatact taaggattcg gaggagtctg aaggaatgcc
     1441 tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga
     1501 tgaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg
     1561 gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag
     1621 cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat
     1681 catcattgag ccgcgcgtca ggttacccat caggctacca acggtggaga agttcgacag
     1741 cgataccagc gaaccatgat ctttatactg ataatttttc agcagcttac cgttcatctg
     1801 cgccagaatg ttgttcattg cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg
     1861 aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg
     1921 atcgcgggtg gtttgcagcg tcagttccac caccagctgg ttgatacggt tagtttcaag
     1981 accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccata ccatcagatc
     2041 agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat
     2101 ggtttgcgtc agaacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat
     2161 acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc
     2221 ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt tgtgcaattc
     2281 agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt
     2341 tgcgcccagg ttggcggagt atttcaggaa gagattcagc atctcctggt gaaagcgacg
     2401 cgcctggtgc gggttatcga ggaaaatgca gttctctttg acacctggtg tattgaaatc
     2461 gttagaggtg ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac
     2521 cagcagttca cctttctcat cgcgcagttc tgcaatagtg attgttttcg cgtcacgatc
     2581 aatatcaatg acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata
     2641 gctcaacgca tcgacgcctt catcaagcga gccagtcgct acttcgtgca gcagcggttt
     2701 ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag
     2761 cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat
     2821 ttttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat
     2881 ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat
     2941 tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg
     3001 ctgaaaagat aggcgacttt accgggtgag atcaaaaaac agagtttatt gtttattatt
     3061 tgagcgaatt tgtgagagtg atatagcgtg aggggtgtat gtagggcgtt cggtaggtct
     3121 gataagaggc gcatcaggca ttgatgctcg actgtcggat gcggcgtgaa cgccttatcc
     3181 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
     3241 ttacccgagg gttttgaacg ctttaatgcg ctgcaaatgc ggggagatat tcttgaattt
     3301 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
     3361 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
     3421 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
     3481 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
     3541 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagggg
     3601 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
     3661 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
     3721 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
     3781 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
     3841 gtaactgatt cagacgggcg ctgatcgctt tccagcgagc cgagtccatc agttcctgtc
     3901 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
     3961 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacccg
     4021 catccagtga tacctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
     4081 ataaatcgtc agagaaaatc acgccgtcaa aacccagttc ctgacgcaaa acggttttca
     4141 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
     4201 gcataatggc gtcgagttta ttttcgcgga ttaacgtgct gaaaaccgac atatctttgg
     4261 cacgaatttc cgcttgcggg cgtgggtcgc acggcgtctc tttgtgtgaa tctgcagtta
     4321 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcatcccat
     4381 caataaaccg actggcgatt gccagggctt tttctgggtc ggcatgataa gaacgctcgc
     4441 caatcgccgc gctgatatgt cctacatcca gcactggcgc aaagctgata tcaatatcca
     4501 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
     4561 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
     4621 aacgctggac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
     4681 cgcggatctg gcgcaccagt tcacgcaact gggcaggatc atgatagttt cgcgtaaaga
     4741 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
     4801 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
     4861 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccggtttg tcgccagcgg
     4921 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
     4981 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
     5041 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctcctgca
     5101 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
     5161 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
     5221 aacattcgca gccaacccac tgtccgccgc gccggatcgc tttgctgcca gtacagttcc
     5281 agtaacggca acagcgttat tcgccagcca aaacgtggtt gttgatgtag ataatacagc
     5341 aagcctgcca gttcgttggt atccggcaaa tacgttttta cctcgccggg cagatagtcg
     5401 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcatgc gggtagttgt
     5461 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
     5521 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
     5581 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
     5641 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
     5701 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
     5761 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tgcctaaact
     5821 atcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
     5881 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
     5941 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
     6001 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
     6061 gcgaatatgc ggcgcaggcg gtgcagtttg atcttcgtgc tcaattgggc ctggctgctg
     6121 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
     6181 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
     6241 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
     6301 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
     6361 gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg cctttccaga ggatgcatct
     6421 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
     6481 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
     6541 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
     6601 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
     6661 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
     6721 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtgtt
     6781 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
     6841 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgag acgtcgttca cagtcggaat
     6901 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
     6961 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
     7021 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
     7081 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
     7141 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatctc cattattccc
     7201 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
     7261 atgcgcatat ttctcatttg catttgcatt tgcatttgca agcaaaatta ttcgcacact
     7321 aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata aaccaagaga
     7381 tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagccat caccaaaccg
     7441 tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt tgctgcacca
     7501 gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga tggcgaaaat
     7561 gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca acgtgatatt
     7621 cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt acaaacgctg
     7681 ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc cgatcgtgct
     7741 ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat ccccacctat
     7801 tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt tgaacgcgta
     7861 gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc tgcggcaatt
     7921 aatatggttc gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc ggcggaatac
     7981 ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac cgaagacggt
     8041 aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct ggaccgctac
     8101 aatagtgaaa agaccttctt ctccggcatt gtcgatgctg atttaggcga tcttacgacg
     8161 ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg gggcggttta
     8221 ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag taccgcacct
     8281 gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa gcagcggttt
     8341 gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt tgacagcaaa
     8401 atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg gccatacagt
     8461 aattatggac ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg caaacgtaaa
     8521 gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg tcagcacaat
     8581 ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt acttcagttc atgggccaac
     8641 atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc acaaaccgac
     8701 tggtcaccac agagcctggc gcaggacgat accacacata tgaaatcgtt atatgctgcc
     8761 actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta taccaactgg
     8821 cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta cgctggtctg
     8881 gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat tttccagccg
     8941 caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa caactacgag
     9001 ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc catcttccgt
     9061 attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag caacggcgaa
     9121 accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga actcaacggc
     9181 gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatattgc agaggataac
     9241 gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt caccagctat
     9301 cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca aaatcgcgtg
     9361 tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag ctacgcgttg
     9421 gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg gaacgtcaat
     9481 aacctgttcg acaaaaccta cgataccaac gtggaaggtt ctatcgtcta cggcgcaccg
     9541 cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa aaaggcagcc
     9601 atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat gtactcatcc
     9661 atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc agaaccagca
     9721 accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt agacacatca
     9781 gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt ttctttacca
     9841 ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgtcgcttt tgcatcttca
     9901 gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac gcggaagatg
     9961 gtgtagtaaa caatcgcata accgataccg acgatcggga acagccacag tttgctgctg
    10021 ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaaa acgacgtacc gtcacgcatc
    10081 cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat gatgtacagg
    10141 atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg tgataccggt caggaacgag
    10201 gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg tttagcagag
    10261 tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa gccaccagac
    10321 agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg gaaaacctga
    10381 cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca gatgtggtgc
    10441 agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc aactaccggg
    10501 ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg cggccaaatg
    10561 aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg cacaaagcgt
    10621 ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg gttaaacatg
    10681 tacgctgcga tcgcaccgga gataatccct ccgagtacgc cagtatccgc caggtgttta
    10741 gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc catggtttta
    10801 accatgatgc cataggcaac aactgcagcc agcgcggata cgccatcgtt attggtaaag
    10861 ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga accgcctgct
    10921 tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga accgacgccc
    10981 agcagaatac ctgcgatagg cagtacggat accggcagca tcagcgattt accgaccttt
    11041 tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta tgggtgcttt
    11101 ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg catctaagcg
    11161 ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac ggctatcacg
    11221 tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt cacaatattt
    11281 caaacgtggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt tagtcattaa
    11341 ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc gtcgatgtga
    11401 aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc aacacctttc
    11461 aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc ttttcctcga
    11521 tgcggcaccg gcgcaaggta aggtgagtca gtttccacca gtaaccgatc cagggggaca
    11581 taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc ggaaaaggag
    11641 atgtaaaatc cgagatccag taatttgccc gccgtttctc tgtcctctgt aaaacagtgt
    11701 agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt atcggcgcgg
    11761 gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg gatatgatgg
    11821 atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc cagcccggtt
    11881 tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc tacatcgtag
    11941 ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc acgttcgcct
    12001 accagatccc gcatgtgtaa ataacccggt aatgttgtgg cgactgccag acaaaatttc
    12061 acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa agattcataa
    12121 tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct cttaaagatg
    12181 aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta aaagatcggt
    12241 gatgagaagc tcgcggttga tgcctgtgac agacattaac cgttcacgaa tgtggcaaac
    12301 atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt cggcgaccag
    12361 gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta gcgcatccat
    12421 cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa gggccgccag
    12481 cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca atgtttcacg
    12541 agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac cggcgcttaa
    12601 gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa gccaggtcac
    12661 ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga caacgactac gtaacgttgc
    12721 cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg gtggctcttc
    12781 aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat cggttaccca
    12841 gacaactttc gcaccaccta agcgtgcgtg ctcattcagc ttttcggtga cctcacgtac
    12901 cgcatcaatg cccagcgtat ttttcccttt ttcgggagct agggtgtagt aatcgggatg
    12961 cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt ggccctgcgg
    13021 ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc ccatgcccgg
    13081 taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg ctaccagttt
    13141 ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt cacccagcgg
    13201 gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc atcaatggta
    13261 tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg attaaagaaa
    13321 tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg ttttaagcca
    13381 acttccgggg taacatcgag atagagcgtt aagtcggggc gaaaatcccc gagaacagca
    13441 tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg atacgcctga
    13501 gtggagagat cgtggcgatc gccaataacc caggtgccgt tagccagcgc aggtttgatg
    13561 accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc tttatcggta
    13621 atgatttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt ttcggcaagt
    13681 tgcgtaccac caggttcccg agtgaaaacc atgtcgcgga tacccagttg ctcgagcgtc
    13741 tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc aatgacgata
    13801 tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag acttgttatg
    13861 actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa aatagagata
    13921 cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg tagctatcgc
    13981 acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt ccaggtctgc
    14041 acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg ggtcggtctg
    14101 caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat cgcgttcact
    14161 ggcaatggcg gtttcttttt cgataattga tgccatcgtc accagctgat ttttatcttt
    14221 ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca ccattttctt
    14281 gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatcc aggtgtctgg
    14341 ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg ctacggtgat
    14401 gtacttatca tcgctcagcg tgtgcttgat atacggggcc tcacgcaatt gcttgaggta
    14461 gtcgctcaga cgcatccctt ctaccagtcg cagagggaac tgtgcttctt taccgctttc
    14521 caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag tcccggcttt
    14581 aaagtgagaa agatccggtt cgatacgcag caaccattga aaaacccgtg ggcgattgat
    14641 gatcttatcg gcataaagct gttcaccaag cgccagacgt ccggtccctg gcttcagggt
    14701 aaatatcgtc tcttctttga taagcaattt gctgtcggca agatagcgaa ccttccagac
    14761 gcccacacca gcggcgatac ccagtaccac cagcaataac aagattatca ataacacttt
    14821 tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa cgttgctgac
    14881 gaaaaggaga catcgccaca ggcacgtacg ggcatcactg gcattaacgc attacaaata
    14941 accatctcat ctgcctgcaa cgcctcttcc agagaggctt gcacttcgac aagctgataa
    15001 gaggattgtg ccagcaaacg gatacagaat tgtcgcataa tgccgttaac acctgcctga
    15061 tccagtcgcg gcgtataaac tacgttgccc ttccgccaga acaaattagc tgcacagcat
    15121 tccgtaaccc ccccttcgct gtcaaggacc agcgcctcat cagcgtttgt ctgctcaaga
    15181 tgagagcgga tcaatacttg ctcaagccga ttaagatgtt taatacctgc aagatgagga
    15241 ttgcgcccca gccgcaccgg gcttagcgcc aacgtgatcc cttcgttacg caaacggtcg
    15301 taatgtgcag gataagccgt aacggagaga atccgcgttg ccggtccact gttcaatgtg
    15361 ctgtaccctc gcccgccact accgcgactg atcacgactt tcagtacacc attttgctgt
    15421 tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca ggaaatcatc
    15481 aaccgctgac aagcatcctg tagtcgctgg atatgcgccg ataacaaact gactttaccg
    15541 tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg atcgcttact
    15601 gacagcgatt cctgcttata accgttaatt aagaacatac cggctcctta tcagagaatt
    15661 gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag aaaaaaggcc
    15721 cgcaagcgga ccttttatat gggtggaaaa tgacaactta gatcttttta aagatcaaag
    15781 aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt ccgctgacct
    15841 gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta tccaggttga
    15901 tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat tctactgcac
    15961 ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac acacggcttg
    16021 cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca gccggcgtag
    16081 aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca tcacgcagag
    16141 catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc atatgataag
    16201 catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg cgttttttcg
    16261 cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg aaaccatcac
    16321 gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta gataatgcac
    16381 gtgccgcgcc aaaaccacca acacccagcg gcgtactggc tttctctgcg ccacctgcaa
    16441 ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg ttgtgcacgc
    16501 cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac atgatagtca
    16561 gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg atcttacgtg
    16621 gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg ccaatcccgg
    16681 agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg ccagaatcct
    16741 gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc ttgcgctgtt
    16801 ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca aatttcgttg
    16861 cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg gcaagcagag
    16921 ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt ccggtcacaa
    16981 ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc tagtgggaca
    17041 aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac gcctggtggc
    17101 cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa
    17161 tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca agagaatccg
    17221 cgcctaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta acgcccagct
    17281 gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta aatttcctat
    17341 caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa
    17401 atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc caataacgca
    17461 aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca tgagcatttt
    17521 gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt tcacccgtga
    17581 tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc
    17641 cgaggcgacc cgcaggaacc tgcaccagga tacccgcacg ctggtcatcg ctcagcgcac
    17701 gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata ccgcgtgacg
    17761 caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt
    17821 tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata atacgaccat
    17881 gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca gatgaaaggt
    17941 tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg ttatcacgag
    18001 tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga attttttcca
    18061 gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg ccgttagcac
    18121 ctaaataatc actgatcgcc tgagcgccat tttcactggt cgcagtgcca ataactttcg
    18181 cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt gcaccggtta
    18241 ccagtgcgat ttttccttca aaattcatga tgttcctctt ttaaagctcg agcgccgctg
    18301 ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg cgtttcgtca
    18361 ggccagtaag cactttgccc gggccgactt catagagatg ttctacgcct tgcgctgcca
    18421 tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac
    18481 gaatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca ggaactgttg
    18541 gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct ggtttcatca
    18601 gcgcacagtg agacggtacg ctcactggta acggcagagc gcgttttgcg cccgcagctt
    18661 tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc acctgtcccg
    18721 gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca cacgcttttc
    18781 caatagacgc atcatccaga ccgatgattg ccgccatagc gcccgttcct tccggtacgg
    18841 cttcttgcat gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg aaatcaatca
    18901 caccagcgca aaccagcgcg gagtattccc ccaggctgtg accggccatc attgccggtg
    18961 ctttaccgcc atgctgctgc catacgcggt acagcgcaac agaggcagtc aacagcgctg
    19021 gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc agcgcccaca
    19081 ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt ggatagctcg
    19141 ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac acaaatgcaa
    19201 attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc caggtgaatc
    19261 caccgccaaa ggcttcaagc agaaccaact gccccggctt gatgcgcccg tcgcgtacag
    19321 cttcatccag cgcgcacggg acagaggccg cagaggtatt accgtggcga tccagcgtca
    19381 ccacgacatt gtccatagac ataccgagtt ttttcgccgt tgcactgata atacgcaggt
    19441 tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattc gccgccagcg
    19501 tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg ttgcccgcca
    19561 tcgtcagatg aattgaattc tctggattca cgcggtcggc gtttggcagc gtcagcaatt
    19621 caccataact accgtcggca tgcagatggg tggaaatgat tcccggctct tcagaggcag
    19681 ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacga tctgttggat
    19741 cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc gccccagatt
    19801 tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt
    19861 caaatgccgg gcaaccttta atgcccaaca tgctttgaat ctgacaagct gcgctcggga
    19921 aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct ttctcaatgc
    19981 ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg
    20041 gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg tcagaggtgt
    20101 ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag ctgccagtac
    20161 caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg tcacttgcaa
    20221 actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc accgtccagc
    20281 agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg aggaacttgt
    20341 cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg attggctgca
    20401 ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt atactggtcg
    20461 gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt taataacagt
    20521 agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag gaacatcctg
    20581 acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca aaccagcaca
    20641 tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt gatagaaggg
    20701 attgttttaa gcaccgctga ggcatcccga atactatcga gaccctttac ttcttcttca
    20761 ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc agccaggact
    20821 gagcccataa tggcaaattg caccaacatt gtgctgtcac aatcgacgtt ggcccctaag
    20881 tcaaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag cgccggacgc
    20941 tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc cccggtatta
    21001 ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc cacgcgcatt
    21061 gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc gataactgac
    21121 tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc aagtaatggc
    21181 gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga attcagtgcc
    21241 tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac atctaacgcc
    21301 agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca gttgcctggg
    21361 gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg accttgcggc
    21421 cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca gaagttttgt
    21481 ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg
    21541 aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta cggtttatcc
    21601 ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg gcaaatgggt
    21661 ttggcttttg cgcttcttca ggcagttcac caaagaccat gtccgcttcg gacacttcac
    21721 agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca tcttcaacca
    21781 ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac gcttccggca
    21841 gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg tagacctgat
    21901 gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc gcatcgccgt
    21961 ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc acatcactgt
    22021 ccacactgac tacggattcg gcgacgcgct caacctgatc aggagtatag ataccctggt
    22081 aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat tttacctttt
    22141 gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag gcagcctgtg
    22201 gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt cgttcagtgc
    22261 tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta tcatcaatac
    22321 gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct cgccctggcg
    22381 tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcacccg aggtcgacga
    22441 aactccacac agcgacgaat caccgcgaca gttggtgctt cgactggcac aagaaaaagc
    22501 gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc aggtatgtgt
    22561 tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc tgcaattacg
    22621 caaagccagc ggcaatatcg tcactttcta tactggactg gcgttgttta attcggcgaa
    22681 tgggcatctg caaacagaag tggagccttt tgacgtccat ttccgtcacc tgagcgaggc
    22741 ggagattgat aattacgtgc gtaaagagca tcccctgcac tgcgcgggta gctttaagag
    22801 tgaaggattt ggcattactc tgtttgagcg tttagagggg cgtgatccta acacgctggt
    22861 tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa acccgctgat
    22921 gggatagttt tttgcgccgg atgcggttac gtcgcatccg gcaatcggtg ctggcggcct
    22981 gatgcggcgt aaacgcctta tcccattatc atgcttatat gctcgtatta gcgcgcgtta
    23041 cgcagctttt gcaaacaacg cttcaaacca tcatccatcg gcgcttcgat acgcatcacc
    23101 tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag acgatttaat
    23161 cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg atcgtcaaag
    23221 gcaatcggat gacctgcata ctgtgtatgc acacggatct gatgagtgcg ccctgttacc
    23281 ggactacaac gcaccagggt ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc
    23341 gacggtttgc cttcctgact cacacgcacg atacgttcgc cgctttgcag aatatttttc
    23401 aataacggcg cctgaacgct cttcacatgc gactgccact gaccgcgcac cagcgccagg
    23461 taatcttttt gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt
    23521 tttttcgcta ccagcaacac acctgaggtg tcccggtcaa gacgatgaac cagttcaagg
    23581 aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcaataa cgccgaagct taaaccactg
    23641 ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatgtgatc atcttcatat
    23701 aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc
    23761 cgctcagcaa cgcgaaccgg tggaatacgc acctcatcac ccgcttcgag tttatattca
    23821 ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg gtaaatcata
    23881 ctttttggta cgcctttcaa ttgggtacgc aaaaagttgt cgatacgttg ccccgcttcg
    23941 tcggcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt gggcgattct
    24001 aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt tttgcacctt
    24061 attacttcac tacgtgatca ctttattgat ggttattaaa ccaatcacca gcaagaagtg
    24121 aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg
    24181 cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa
    24241 taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc
    24301 aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag
    24361 taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa acgggcaacc
    24421 gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg
    24481 aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa
    24541 gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt
    24601 gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa gtccagggca
    24661 cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga
    24721 agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc
    24781 ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt
    24841 gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc
    24901 ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc cgaacaaccc
    24961 gcgcgcgggt ggcatttctc gccgtatcga aggcgacgac cgtaccgaat taaaagaagc
    25021 actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg
    25081 caaatctgct gaggcgctgc aatgggattt aagcttccgt ctgaaacact gggaagccat
    25141 caaaaaagcc gctgaaagcc gcccggcccc gttcctgatt catcaggaga gcaacgtaat
    25201 cgttcgcgca ttccgcgatt acttacgtca ggacatcggc gaaatcctta tcgataaccc
    25261 gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggtcgcccgg atttcagcag
    25321 caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc cactaccaga tcgagtcgca
    25381 gatcgagtcc gccttccagc gtgaagtccg tctgccatcc ggtggttcga ttgttatcga
    25441 cagcaccgaa gcgttaacgg ccatcgacat caactccgca cgcgcgaccc gcggcggcga
    25501 tatcgaagaa accgcgttta acactaacct cgaagctgcc gatgagattg ctcgtcagct
    25561 gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga cgccagtacg
    25621 ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat
    25681 tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgccagc gcctgagccc
    25741 atcactgggt gaatccagtc atcacgtttg tccgcgttgt tctggtactg gcaccgtgcg
    25801 tgacaacgaa tcgctgtcgc tctctattct gcgtctgatc gaagaagaag cgctgaaaga
    25861 gaacacccag gaagttcacg ccattgttcc tgtgccaatc gcttcttacc tgctgaatga
    25921 aaaacgttct gcggtaaatg ccattgaaac tcgtcaggac ggtgtgcgct gtgtaattgt
    25981 gccaaacgat cagatggaaa ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga
    26041 aaccccaacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc
    26101 tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat
    26161 gccggatgtg ccgccagcgc caacgccagc tgaacctgcc gcgcctgttg tagccccagc
    26221 accgaaagct gcaccggcaa caccagcaac tcctgcacaa cctgggctgt tgagccgctt
    26281 cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccga ccgagcaacc
    26341 agcaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa
    26401 caaccgccgt gaccgtaatg agcgccgcga cacccgtagt gaacgtactg aaggcagcga
    26461 taatcgcgaa gaaaaccgtc gtaatcgtcg ccaagcacag cagcagactg ccgagacgcg
    26521 tgagggccgt cagcaggctg aggtaacgga aaaagcgcgt accgccgacg agcagcaagc
    26581 gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc
    26641 gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg
    26701 tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag
    26761 cgtagccgaa gaaacggtag tcgcaccggt agctgaagaa actgtcgctg ccgaaccaat
    26821 tgttcaggaa gcgccagctc cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc
    26881 gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca acggtggcat
    26941 gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg
    27001 ctatcgtgac gagcgttatc caacccaatc gccaatgccg ttgaccgtag cgtgcgcgtc
    27061 tccggaactg gcctctggca aagtctggat ccgctatccg attgtacgtc cacaagatgt
    27121 acaggttgaa gagcagcgcg aacaggaaga agtgcaagtg cagccgatgg tgactgaggt
    27181 ccctgtcgcc gccgctgtcg aaccggttgt tagcgcgcca gttgttgaag aaatggctga
    27241 agtcgtagaa gcccccgtac cggttgccga accgcaaccg gaagtggttg aaacgacgca
    27301 tcctgaagtg attgccgccg cggtaactga acagccgcag gtgattaccg agtctgatgt
    27361 tgccgtagcc caggaagttg cagaacacgc agaaccggtg gttgaaccgc aggaagagac
    27421 ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt
    27481 tgctcaacct gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtaaccgc
    27541 ggttaaacca gagatcaccg ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc
    27601 ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc
    27661 cttcgaaggt aaaggtgccg caggtggtca tacggcaacg catcatgcct ctgccgctcc
    27721 tgcgcgtccg caacctgttg agtaataatt agctcaagta atcaagccct ggtaactgcc
    27781 agggcttttt tatttcatct ttgaatcaac atattaaaat tcatcgaatg gcatccttgc
    27841 taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg gcgatccctg agcgggcaga
    27901 caaaacatac ccagtttcat gatatgtttt aaagcgagct tatttgttga gctggaagag
    27961 cgacaatccc tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat
    28021 gatgtaagat gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt
    28081 ttgccctaat gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagctgcgt
    28141 gcctaattcc gcgcgaacgg tcaggacatt gttcagcgag tttttcagtc cgcggttggt
    28201 tttatctaac gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt
    28261 cagggctgcg atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc
    28321 tggttccgct accgcgttac tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat
    28381 caccatcgaa cgcgaagcat cgacctgttg cttaatactt tctgttccgc cgacataatc
    28441 accgttaacc tcactaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc
    28501 gttaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct gaatatccgt
    28561 agccagcgag gcccggtcat tgtcactcaa ggtgccattg ctggcgtaga caattttttc
    28621 ctgagcattc tggatagcgg tggtgacctg gctaagtaca ctctcttcca gtgacacttt
    28681 ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag
    28741 aacaacagct tgtgatgcag caatgggatc gtctgaaggg ttaacgactc gcttacccgt
    28801 cgacatctgc tcgccgtact tcatccattc tgcctgagaa ttggtgatgc cgcgcatgtt
    28861 ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg aatgttaatc
    28921 agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc
    28981 tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc
    29041 tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt caacgtcgcg
    29101 gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgctccg
    29161 cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta
    29221 tcacccgcat cttcttcgct cgccatcgct attttcgctt cgtcggtgat taatacatcc
    29281 atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt
    29341 cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc tggcgtcacc
    29401 gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc gaacgagatt
    29461 ttgtaatctg tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtta
    29521 ccgttgtttt tagtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcaccc
    29581 tcatcgccgt tggcatcaaa tccggctttg tgttggctgt tgaaagcctc agcaaatgcc
    29641 agcgccagtt gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga
    29701 atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc
    29761 ccatcaacat aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt
    29821 tgccgcgccg tacttccctg aaccagtgag taaccattgg ccatcgtgat gttataagtg
    29881 ccgccatcct gaacgctgac ttctacacca acaatctggt ttaattcgct caccagttga
    29941 tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgggag
    30001 atttgatcgt tcaggctggc gatttgttta gcgtaattgt tgatctgatc aacgctggca
    30061 cctatcgcga tattgacctg tttgtcctgg tcgcgaagat actgatcggt ggttttaaac
    30121 tgattcacca acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg
    30181 ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag
    30241 gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg
    30301 ctactttgcg tctgcgccgc acgtaactgg ttggtaataa acgcatcata ctcacgctgc
    30361 acaccagaaa cgtagacacc attaccaacc cagccgccag cgcccaacgt gctattggcc
    30421 tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta
    30481 cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag
    30541 ctggacatgg aggttcctta taagccttca attacagagt attatcggca gcgacccgcc
    30601 ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt
    30661 cgcttatcga tttcatctgc tgaatcatgt tggtgagttt acgggcatag tgaggatcgg
    30721 tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca
    30781 cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat gcttccagat
    30841 acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccattt tcatattcag
    30901 tcgtggtgat ttcagtgact ggccctttcc agttgccaga ggctttgaca ccaaacaggt
    30961 tatagctcgg ctcgccgttt tcgcggcgga tttgccgttg cccccagcca gattccagcg
    31021 ccgcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg
    31081 gcagcgagag ttgcgcgagg aatgctttac tgtcacctgg cagcgaatca tcgtagttac
    31141 gtggcacggc cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacggttt
    31201 cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg
    31261 tcatctgctt aaccatcatc tctgcaagcc ccagaccttt gcccgtcgtc atctgttggg
    31321 caatctgctg gtcatacata ctggtataca ggcgagtgtg ctcgctgctg aacaggccat
    31381 cttttggtaa cgcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct
    31441 gacgggccac cggacggata tttgccgccg gatcttcgct cgctttcgcc tttagttcgt
    31501 tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat
    31561 gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcaaaa tagacatcag
    31621 atccatcggc gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct
    31681 ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaag tcgatctgcg tttgtggcgt
    31741 taccacggtc tgtccgccac caaacggtgt atctggttgg ctgacattgg cctgacgatt
    31801 gactgttact gagagatttc cctgcgctat cgcgcagctg tcgagagtca cttcgcgatt
    31861 catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt
    31921 aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc cactcggtac
    31981 gcgcacctga atagtccgcg catccaacgc ggtggcgctg ccatatccac gcacgcggtt
    32041 gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag
    32101 ggtattcccg acgccaaact ggctgggtaa ttcacgttca ataactgcac cattggtgat
    32161 ccgtccaccg ttcagttggt tcacctgaac actgctaccg ccagcggagg ctcctgcgcc
    32221 gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg
    32281 agtcatcaac aatgtgccgc cacgcaggct tttggcattt cccatggaag aaaccaccac
    32341 gtcaatggtt tgcccctgac gtccaaacgg tggaagtgac gccgtcacca ttaccgcagc
    32401 aacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat
    32461 gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag
    32521 ccccaccacc agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag
    32581 atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag
    32641 aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac
    32701 cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc
    32761 gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca
    32821 gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc
    32881 aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtatta
    32941 ctggcattgg ccccgccttt tccgttgaac gtgttaccac cggaggcttc gacatcggca
    33001 cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt
    33061 ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctaacgtt ctcctgcaac
    33121 acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag
    33181 ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcgtaccg
    33241 ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag
    33301 ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc
    33361 atggctatca tcttcaaaaa tcaataaacc ggtgaacctg ccaccggtta tgccttagag
    33421 ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta
    33481 agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc
    33541 aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc
    33601 accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt
    33661 cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt
    33721 tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat
    33781 cgctggctgc acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa
    33841 agagccgtca cgggtatagg ctgatgagcc atctggcaac atcacctgga aaaagccctg
    33901 ccctttaatc gcgacatctt tgctgttgtt ggtctgcgac aggttgcctt ggctgtgtaa
    33961 gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt
    34021 ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac
    34081 cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc
    34141 catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa
    34201 atgtcctgta gtggattgct gacaagcaga gcagaagcac gcggcggtgc gaatttcccc
    34261 tcaccctaac cctctcccca aaggggcgag ggaacagtcc gctcgctttt attgcaatgt
    34321 catcaatcgt tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg
    34381 acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc
    34441 attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt
    34501 ggatctgcct gcagtaccgg tccacgcgtg gcctgggttt ctgcgcttaa acgaaaaatg
    34561 ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttcag acgccccact
    34621 ggcgcaaccg tatttgccgg atcgcccgga ttcagcgccg agattgttcc atcggcagct
    34681 atcgtgattt ccgccccttc cggcacagca attggccccg cctcgcctat caccggatgc
    34741 ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct
    34801 tcgctgccgt cagcggtctg cacggccaac cagccatcct gctgcaacgc aacgtccagc
    34861 gggcgcgagg tgtaatccat cttgccgggc gttatgtctg cacccggtgt tgacgccgtt
    34921 accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac
    34981 tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga
    35041 ttcagcgtct ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc
    35101 cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt
    35161 ctgggcgtta gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag
    35221 atcgacgttg gacgcttcca acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc
    35281 tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga
    35341 tgccagacct tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgggtttg
    35401 ttcgttggaa tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc
    35461 gcccggtttg tagccattct gggtggttgc cacaatgttg ttagcgccgg tattttgctg
    35521 catggagttg aggaagctca ggctaaacgt ggcgggttct gcgccgttaa ttgcgccggt
    35581 ggtaatattc tctgttggat ttgaggtcag aacgccattg gcattaaaca ccagcttcat
    35641 tgcaggctcg gctgtacctg ttggatcact gctatcctgg gtgtagacct gccagttatt
    35701 atccccggtc ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    35761 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcatcaaatg cgttaacaga
    35821 gggaagcgga tcgctggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc
    35881 tgccatcagg gtattcggga tcgaaatgtt agtcggattc gccccttgct gaatagtcgg
    35941 cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg
    36001 attttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctatctac
    36061 cagacggaaa aaaccgttct ggctgatagc aacgtccaga ccacgcccgg tgttggtggt
    36121 cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    36181 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    36241 ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc
    36301 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    36361 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgttgt tgccgcggat
    36421 cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact
    36481 ggcgctaatc gctacgttgt aagaaccgtt cggcgcagtg ctgccgtcag tcaacgtacc
    36541 gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac
    36601 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    36661 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    36721 ggtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt
    36781 atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt
    36841 gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    36901 gtttttcagc tgcgccacca acaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    36961 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    37021 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    37081 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    37141 ttaaccatct ctccgacaac atcaacgttc ggcattttta cgtagccctt tgcatccgcc
    37201 agcggattac ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca
    37261 acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt
    37321 gcccgatatg gctgtccatc aggaccggtc acgctatcag cattcgccag attactggcc
    37381 gccacgttca ggcgctggga ctgggcagtt aacgccgatc cggcgatatc aaaaatattc
    37441 agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg
    37501 ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg
    37561 cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt
    37621 tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt gcgttgaggt catcgtcaga
    37681 gcaaccacac tggttgcatt ccgtccacgt tgcatgactt ttttaagttc actggcaaaa
    37741 tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact
    37801 tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc
    37861 ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac
    37921 gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat
    37981 tgtggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct ggcaataaaa
    38041 cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtgcggcgag caatctcaca
    38101 tcgcaattgc aaaccttttt tagctcccag ctcgcaggga taagtgatga ggttcgtgtt
    38161 tctattcgta cagcgcccaa tctactaccg ccgtgcgagc agccattgct ttcgatgagc
    38221 aataattccc gcttgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga
    38281 tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcccattgtg
    38341 cgaggaggaa agctgggagc tggcaatgtc aaactgaaac gcggacggct ggataccttg
    38401 ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta
    38461 tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga
    38521 caacgcgtca acgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg
    38581 ctaaacaatg cagccgtcgc acagaatgcg cgggtgcgca tggtatctgg acaggtagtc
    38641 agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac
    38701 ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc
    38761 atgagtattg atcgcacttc gcctctgaag cccgtaagca ccgttcaacc gcgcgaaacc
    38821 actgacgcgc cggtaacgaa cacccgagcg gcaaaaacaa cagcctccac cagcaccagt
    38881 gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt
    38941 gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc
    39001 aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa ttaatcgtat
    39061 gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt
    39121 aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg
    39181 gattacagaa caaaaaagct cactgctggc aacgttggat tacctcgaac agttacgcag
    39241 gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattgctgt
    39301 gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat
    39361 tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg
    39421 ggcgaacggt cagacctcaa caacccatcg cggcagtaaa aagatttcga tctgaagatt
    39481 atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt
    39541 taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga
    39601 ccgccattaa acgcagcaaa cgccagggca tggtacccaa tgaccactcc ggcatgatat
    39661 gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa
    39721 acgccatcca gccaggttgc ggggtaaaga ttttctgctt acgcaactgc cagtaaagca
    39781 gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg
    39841 gaccgataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca
    39901 ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca
    39961 ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaagg
    40021 cggtaaattt accgtactgg aacagcgaaa cggttaacgg accggaaaga atgcccaacg
    40081 caaccgcact cggcaacgcc aacaggaaac aaagacgcag tccccagtcc atcaaacggt
    40141 tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggtagc aaaatggtgc
    40201 caagcgccac gcccagcaca ccggacggaa actccattaa gcggtcggcg taatacatcc
    40261 atgacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct
    40321 ggctcacaga gacgccaagg attgccggtc ccatctgttt caccacacgc attgctccgg
    40381 catcgtggaa gttaatgcgt ggcaaaacca gcatgccgat cttcttcaga tgcggtagct
    40441 gatacaccag ctgcaggacg ccgccgaccg tcacagccca cgccagcgcc agcaccggtg
    40501 ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg
    40561 gagcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg
    40621 ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa
    40681 atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga
    40741 gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga
    40801 caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa
    40861 atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag
    40921 cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc
    40981 gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta
    41041 ataaattcat cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg
    41101 aaagaggcga atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt
    41161 tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cactgcctgt
    41221 tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag
    41281 ccgacaaatc cacgttgctc aagcgtgctc tgccaaccag gaatcggttt attcactaca
    41341 ccctgcccac gctcctcacg ccattcgcgc atttccgtaa tgtcgatgag cgcaccgtca
    41401 gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca agtggtgatc
    41461 tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc
    41521 agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc
    41581 acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta
    41641 cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa ctcacagtag
    41701 agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt
    41761 tccaccagcc gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt
    41821 acccccgcat tgagtaacgt actgaccacg tcaaagtgac tggcggtgct ggaatgtaca
    41881 aaaaccgcat cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag
    41941 ctttcacaaa ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacgtccag
    42001 tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccact
    42061 acgccgatac gtaatttttt cactttaatc tcccaggtgg gccagcaacg aatcaagacg
    42121 ctgtttcagt tctgccactt cgatttccag ggcttcgacg cgggcctgta aatcaccgtc
    42181 aaccgcgttc gacatatccg ttaccgccgg ctgatcctct acctcaccac tgaaaagatg
    42241 catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc
    42301 ttcgcgattc gccagttgtt ccagcgtcaa ctccacttcc gccatatcgc tgaattcata
    42361 cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca ataacaacgt
    42421 ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa
    42481 acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg
    42541 tttgaccaga ttatccagct gttcctgcac ttcagattca ctcagattca tgaccggttc
    42601 acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc
    42661 cggcgtggtc acctgctttt ccagcaagca accaatcaca cgagcttcca gggcagtaag
    42721 ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg
    42781 tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt
    42841 tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag
    42901 ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt
    42961 caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat
    43021 gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg
    43081 ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccca
    43141 gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct
    43201 tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca cgatcatgca
    43261 ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc
    43321 caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata
    43381 cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt
    43441 tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg
    43501 ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg
    43561 aaatgtcccg cgtgtcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata
    43621 tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc
    43681 aaatgggctg ggccgccgtc atggtcggta ttgccctcgg cctacgccag tttattcagc
    43741 aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg
    43801 ttaccggtat gctgatgcgt gccgccggat tcgccacaat gggtatcgcc cacgaaccgt
    43861 ggctattgtg gttttcatgc ctgctctcgg gactcggtgg cacgttgttt gatccgccgc
    43921 gttcggcgct ggtggtgaaa ctaatccgtc cacaacagcg tggtcgtttt ttctcgctgt
    43981 tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc tggctgttgc
    44041 aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgctg tgtgcagcgt
    44101 tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag
    44161 gcatgacccg cgtgatgcgc gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt
    44221 actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gatgtggctg
    44281 gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc
    44341 tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg
    44401 ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac
    44461 ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa
    44521 ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc
    44581 tgggtctggc gattggcggc gctattggtt atatcggtgg cggctggctg tttgacctgg
    44641 gcaaatcggt gcaccagcca gagcttccgt ggatgatgct gggcattatt ggcatcttca
    44701 ctttccttgc gctgggttgg cagtttagcc agaaacgcgc cgcgcgtcgt ttgcttgaac
    44761 gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca
    44821 aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc
    44881 gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg
    44941 cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca
    45001 gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc gacggcaaac
    45061 cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct
    45121 acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc
    45181 ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc
    45241 tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttacgt gcgctggaca
    45301 aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat
    45361 tcccgctact gcgtaatctg acgctggtag ccggaattaa ctggccaagc cgcgttgctg
    45421 attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt
    45481 aatcctgact cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg
    45541 ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc
    45601 atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg
    45661 ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg
    45721 cttgcgaaac ataataattt ctcaaaagat attggtttac ccggtgtggc tgacgcccat
    45781 attgttctga caaacctgac cagccaaatt ggtcgcgaag agccgaataa ggttgcccta
    45841 accggagacg ccaatctgga catgaactcc ctgttcggta gccagaaagc gaccatgaaa
    45901 ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gcgcgatctt cctgaaagag
    45961 atggaggtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg
    46021 cttccctatc tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg
    46081 cgcgaagatg gcagccaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg
    46141 aagccaggcg aaattgtcat tccatttact gattaatcac gagggcgcat tcgcgccctt
    46201 tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct
    46261 tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca
    46321 gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc
    46381 agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccaccgtt
    46441 gaggctgccg tggcgtatcg ccaacgtatt cttcacgccg tacctgccgg gcacgatttc
    46501 accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga
    46561 tttaacgaag gcgtgttcac cgctgcaaaa ctttacccgg caaacgcaac cactaactcc
    46621 agccacggcg tgacgtcagt tgacgcaatc atgccggtat tagagcgcat ggaaaaaatc
    46681 ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt
    46741 gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac cgcgctgaaa
    46801 gtcgtttttg agcacatcac caccaaagac gcagccgact atgtccgtga cggaaatgaa
    46861 cggctggctg ctaccatcac tccgcaacat ctgatgttta accgcaacca tatgctggtt
    46921 ggtggcgtgc gtccgcacct gtattgtcta cccatcctca aacgcaatat tcaccaacag
    46981 gcattgcgtg aactagtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg
    47041 ccacatgcac gtcatcgcaa agagagcagc tgcggctgcg cgggctgctt caacgcccca
    47101 accgcgctgg gaagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa
    47161 gcattctgtt ctgtaaacgg cccgcagttc tatgggttac cggtcaacga cacattcatc
    47221 gaactggtac gtgaagagca tcaggttgct gaaagcatcg cactgactga tgacacgctg
    47281 gtgccattcc tcgccgggga aacggtacgc tggtctgtta aacaataaaa atcgtagctt
    47341 cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat
    47401 tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc
    47461 cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt
    47521 atcagtacgt tatgccgcag cgaataattt atcggttatt ggcgcgacaa aagaagataa
    47581 acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag
    47641 cgaataatat gcagtgattt ttgtttttgc cgggtcgccc cggctttttt attttacttg
    47701 cgaattatga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa
    47761 atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt
    47821 ccccccttta aaaattcggt gattaccctt atttattggt atactgaagt tgcttcagat
    47881 aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg
    47941 aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg
    48001 atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg
    48061 aagggactga agttggtcag acgctctggt taaccactga tgttgccaga caatttattt
    48121 cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc
    48181 ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat
    48241 ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga
    48301 aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt
    48361 attatgcaac ccgcgccggt ttaaacgtgc ttatgaccga cgcccatatg ccaccacatc
    48421 aacacggcag ccaccacggc gatacgcgat taattcgaca tgcttatggt gaaggcgaaa
    48481 agtatgtccc gctggtcctc cgcgcacaaa cgctgtggga tgacctctcc cgccacaacg
    48541 aagatgatcc catttttgta cgctctggtg tcattaacct tggcccggct gactccgcat
    48601 ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg
    48661 cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat
    48721 ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg
    48781 cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg
    48841 atgatggcgt aactattgaa acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct
    48901 gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag
    48961 tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta
    49021 ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga
    49081 agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg
    49141 cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta
    49201 tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta
    49261 ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg
    49321 gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa
    49381 gcgattttga tttgacgcca ttcagccttt cccgcttcca ataatcatca ttacggcctc
    49441 cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa
    49501 taatattcgc gagcatctga tgctatatct ttttctctgg ggattgttgg caattatgga
    49561 tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat
    49621 ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc
    49681 agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa
    49741 ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc
    49801 gacacgccat tttctttctc tcacaaattc gttttggatg cacttatgtc attcacaaat
    49861 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
    49921 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
    49981 cacaaagcac ccgaactaca taaaagcatc ggtattttgt taatgatggg gctggttatt
    50041 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
    50101 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
    50161 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
    50221 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
    50281 ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag
    50341 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
    50401 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
    50461 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
    50521 gttaactttc gcatccagca tcttggctat agctggttat atggcacctt taaagatttc
    50581 gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt
    50641 aacaccacca gcgtcgatac taatcacgcc gaacgtgata aacatcttcg cagtgcagat
    50701 ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
    50761 ggtgacgaac tggatattac cggcgatctg acgctgaatg gtgtaaccaa acccgtcacg
    50821 ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc
    50881 gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct
    50941 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat
    51001 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt
    51061 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
    51121 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ttcactacaa
    51181 caacctttgt atttttccgc acatactgga cactgaataa acagcaggtg gcagccatca
    51241 tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc
    51301 tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
    51361 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccacc ctcgatatgc
    51421 cagactttat tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
    51481 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
    51541 ggcagctgct cacggaaggt atctgccgga atttccagcg cgttttcaaa gtgccccact
    51601 tcatactcat agtggttacg catgtcgata aacagtgcat cgggatcgtc aagcatggcg
    51661 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
    51721 ataccgtcgg caacgatacg atcgcggacc ttcatgcgca gtacccagaa ggatttcccg
    51781 tcatcatcca acgcgatatt caggcgtaag ccatctaaag ccgagtcgaa ggcatagagc
    51841 tgcgcgcgaa atgttttaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
    51901 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
    51961 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
    52021 tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact
    52081 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
    52141 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
    52201 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
    52261 atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct
    52321 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
    52381 cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt
    52441 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
    52501 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
    52561 atccgttggc atgggactga tggaaactgg catggcgtgg ttctggccgg accgccgaat
    52621 cgctcgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
    52681 cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg
    52741 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
    52801 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
    52861 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg
    52921 cccgcgctca agcgttttcg tccctttatt tgccgttgag caggctgcga ccacgaccgg
    52981 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
    53041 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccgctgga
    53101 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
    53161 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
    53221 acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa
    53281 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
    53341 ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
    53401 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
    53461 gctaggctgt tttcttaccg gtgccgcctt cagtctggta atgcccttct aacccctcta
    53521 cgttgagcag cttggcgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt
    53581 cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg
    53641 taaaggccga aaactcatgc tattacgctc tgctctcggc atgggcatcg tgatggtgtt
    53701 gatggggctg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttgggttact
    53761 tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag
    53821 cggctgggca ctgggtacgc tctccacagg cggcgttagc ggtgcgttgc tcggcccaat
    53881 ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag
    53941 tgtgctcata ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt
    54001 cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact
    54061 ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacgggct caattgcccc
    54121 cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag
    54181 tggcatgatc gcctcggtgc caggcgtagc tgctctgctg agtgcaccac gactcggcaa
    54241 acttggcgat cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact
    54301 gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt
    54361 gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc
    54421 gagcaaccaa atcgccggac gtatcttcag ctataaccaa tcgtttcgtg atattggcaa
    54481 cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt
    54541 tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg
    54601 tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga
    54661 atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt
    54721 acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg
    54781 gcatcgacgc agaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg
    54841 acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt
    54901 tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag
    54961 agatccgcca ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc
    55021 agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac
    55081 gttaattact catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc
    55141 agggagaatg gcagatcaag gattgtgaca taacgccagg cggagtcacg cacatcccat
    55201 tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg gctaatgatg
    55261 ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac
    55321 ctcttccgta aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc
    55381 agatgtggcg tgaacgcctt atccgaccta caaaatcttg caaaatcaac acattgcagg
    55441 agcgatgtag gcctgataag catagcgcat caggcaacta cgtttttatt gcgaagccgc
    55501 atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga
    55561 agaatatctc tcaggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc
    55621 gcttagcagc acaaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg
    55681 ttcaacatgg cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc
    55741 cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa aaccatcatc
    55801 aagggagcgt tgacggttca tctcaaggaa ccgatcggta tcaaccagta cctgcggcgg
    55861 tgaatactct tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc
    55921 acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag
    55981 ccagaacagg aaacgcagat ccaaccacgc catcccgaca gcccacacta accctaacag
    56041 cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg
    56101 cggtgaattc cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa
    56161 cagcatgcgt accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt
    56221 aacgcgccag aagccgccat attctttcgt tcctttacac caaataagca aaatgctcaa
    56281 tagctttggc aggaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg
    56341 acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt
    56401 caacgcatgc actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc
    56461 ggagagataa gacatcacgc ccgtcaggaa taccgcacgg tgaaccgggt gcataccctt
    56521 caccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag
    56581 ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca
    56641 gactccccaa cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg acaggattga
    56701 accggcaaag gaaccttcgc ccggcagcgg ggccagtgca cagtgctcga taaacggttt
    56761 cacgcggata atcgcgttat gcccccagta gtgcgactcg ccaagttgcc agaagtgcaa
    56821 accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gtgcatacag
    56881 cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc
    56941 catcaggcgc accaggccgc acaaacaatc accggtcatt accgagtcag catccagcac
    57001 caccatatag ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt
    57061 acgcttcacg cgacggcggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc
    57121 gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttgt aactgtcact
    57181 cagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc
    57241 acgcaagcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt
    57301 gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc
    57361 gcgaccaata agtagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca
    57421 acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg
    57481 cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc
    57541 ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag
    57601 gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg
    57661 ctcttctttg gtcaaacgag ccagatagcg cggtgttaca tcgcgtccac gcaggcgatc
    57721 ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg aacgttttgc
    57781 ttctggcatc gccttaagct gatcgcgccc ttcgtcatct ttaattaact gtccatcagc
    57841 aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg gggagtcatc
    57901 ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt
    57961 cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt
    58021 cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca
    58081 ccagcgcagc gcgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca
    58141 gacgccagcc tttggtgacc gggttatagc gcaccgtgct ttcaactatc tcaccattat
    58201 caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc
    58261 cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct
    58321 tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt
    58381 tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg
    58441 gcagctgatc cggcgtccag taagcgacga tgttatcgtt agtttcatcg ttggttggaa
    58501 tttccaccag ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg
    58561 gacggagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca
    58621 acagaccaaa gccttgcggg ttttccatgg agaagctgct gaccgctaaa tgtttcgggt
    58681 tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt
    58741 cgtgcaactc cggacgatag ttatttgctg gcgacggttg gttcggccca aacaggaaca
    58801 tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact
    58861 gcacatccac aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc
    58921 gcggcgagtc aagcaatgca taaatggtta aacgtttatc agttggtttt ggacgctcga
    58981 tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca
    59041 gaccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc
    59101 cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa
    59161 aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga
    59221 aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga
    59281 ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat
    59341 tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat
    59401 cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt
    59461 gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta
    59521 acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta
    59581 gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc
    59641 cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga
    59701 ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg
    59761 gtttgccagc aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta
    59821 gcgatgatgc tatgcataaa aacttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc
    59881 tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt
    59941 taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc
    60001 tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg
    60061 catgtgaata gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt catccactcg
    60121 ttccgcatgc aggtattttt cgttatatcc gggtactttt cctacatgct ttttttacgc
    60181 tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg tagggatccc gatgttaaca
    60241 gccatccccc tactgacatt gccgcaattt attatgctgc aatacgtcaa agggaaagcg
    60301 gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata
    60361 tcacatctgt ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt atggatattt
    60421 aaacgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta
    60481 aaactatcgg tgattttttt gtgcctcggc atcggttatg cggtaataag aagaacgatt
    60541 tttattgtgt atccacccat tctgagtaat ggcacgttca attttattgt catgcaaacg
    60601 ctattttatt taccgttctt tatcctcggc gcactggctt tcattttccc tcatcttaaa
    60661 gccttgttta ccacgccgtc tcgtggctgt accctcgctg cagcattggc gtttgtcgct
    60721 tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg
    60781 atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg ccaccgtttg
    60841 cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt tatctatctg
    60901 gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat cacctccaac
    60961 tggcttggtt ttctctgtgg cctgatattc gtagtaggga ttgcgataat tctgtatgaa
    61021 attcatttgc gcatcccgtt actgaagttt ttgttctctg gtaaaccggt tgttaagcgt
    61081 gagaacgata aagcaccagc ccgttaagcc acatttacaa taaccattcc acgggcaata
    61141 tcgacgccag tctgaccata acccgcttcc agaaactggt agcgggttct tttttgagaa
    61201 caatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg tccagacgga
    61261 gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac tgagccagcg
    61321 tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc ggatcgaaat
    61381 tgaaagaacc gataaacacc gttttaccat cgatgctaaa ggttttagca tgcaggctgg
    61441 caccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca cgcgtcggct
    61501 tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg
    61561 catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca atctttaccc
    61621 cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca aaataggaag
    61681 agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca
    61741 gtgaatgccg ttttgccttg ccctcccctt tcgccggatc atcacttaat aaacgtgttt
    61801 tcgcccagat aagcggcaac gttccatcaa ccagatgatt tataaacgga ctggattcca
    61861 ttttgcgtaa gtaacgatgc gtcatggcat cgttatgcca ggaggcgggt aactcgatgc
    61921 gatccgccat ttcaccttcc gggacatcca gcacctgctg taagggtgaa accgatttgc
    61981 aataccagta gcgggcgaaa tcatcggcaa cgtcctctac tacgggtcct attgccatga
    62041 catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa ataggcatca ccaatatttc
    62101 gcccccctac caacgtcacc acgccatcga cagtgaaact tttattgtgc atacggcgat
    62161 tgagacggga aaagtcggtg atataaccaa gcggacgcag caagcgaaac gagaaaggat
    62221 taaaaagccg gacttcaatg cggggatggc tgtcaagcaa gcgtaaaatg tcgtcaagtc
    62281 cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt gctgcggcta
    62341 acagggcgga aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa tactgaacat
    62401 ccagcgtatg ctctgacatt tcggccagac gataccgggc ggcaaacgca tccagacttt
    62461 tctccagagg gaaaagacca cactgaccgg gatgttgcga acacagtggc agcaccgcgc
    62521 tcgccagccg gggcaaatca ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg
    62581 gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg acgggtaata
    62641 aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta accataaacc
    62701 ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgccgccac cagtcgtagg
    62761 ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc acgccagact
    62821 ggcccgacgg tgtgcactac ggctttagca ggaagatcgc ctgcaagcgt aataacggca
    62881 tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta aacaagcatc cagcagggcc
    62941 ggacccgctg cgcgatgaat ggccccatcg acgccgccac ctcccattaa tgaaggatta
    63001 gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac aacatgaata
    63061 cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc aggctttttg
    63121 gttaaatcgc atttttctgc tcagcaacgt gagttgttca gaaacttagt tatattcgat
    63181 gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg aggctatttg
    63241 cgcgtttttg acattgccgc tcattaacgc tttgctggag taggttttac ccggaacaga
    63301 accattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt cacatgccgg
    63361 ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc ctgcgatggc
    63421 aataaaagga aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc tgaacattta
    63481 ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact ttcaggttgg
    63541 ctgattcgca gcagaccatt ctctccagat tcatcttatg ctcgatattt caacaaatta
    63601 agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc catcagaaac
    63661 agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc
    63721 aatgggttga ttagcaggca atgaaagggt cttttcttgc ttcgtctgac tttgccctga
    63781 actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag taagagtgac
    63841 ttcaggaata atggtgtaca tatccccttg ctgggtcgta ttaaaggtta tctgactgga
    63901 aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc
    63961 gccctgtttc tttaatacag atgatgtatt agtactgatg agcggtcgcg ttgttaccaa
    64021 agccaacctg agttacgttg acggtggaat tagatgcagt ctggtcaacc gctgcaccgt
    64081 tgccgccacc gaattgttta actgtcatat gagagtcttt accgttccac tgatcaagag
    64141 tggcgctgtt accaaagcca cgttgggtca gatcgattga gctgtcatct gagccctgac
    64201 caacatctgc accgttacca ccaccatgct gggtaatagt aagatcagag ttacgagcat
    64261 cagcttgcag agcaagtgca gagttaccac caccgtactg ataaatattc agctctgaat
    64321 tcgggccgct gttattaccg ccaccaccgt ggttaccgcc accgccgccg tactgaggaa
    64381 caacacctgc cagagcgcta ccggagaata cgattgctgc aattgctgct acttttaaaa
    64441 gtttcatgta aaacccccat cggattgatt taaaagtcga atggaaatta acgttgtgtc
    64501 acgcgaatag ccatttgcga ctgtctctgc actacaattg ccgttttttg agtaccatac
    64561 tgtgtaatat ttgctttatt accagaacct ttctggataa tcatcgcagt attaccataa
    64621 gcaccttgcg aaatactggc atcattggca ctgcccgcct gatcaatata tgcaaggtta
    64681 taatctcctg tctggtcaat ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc
    64741 aaaagttttg agcctccctg ccgtaactga gcactattat tagtcccagc ttgaccaatt
    64801 atggctgcct gattaaatga agacttactc aattcattta ccgcgaagtt atattctgaa
    64861 ttagctaaat cataacctgc tgcggctgca atcccaggcg cacccagtat tgttaacatc
    64921 ataaataaca atttgttttt catgttgtca ccctggacct ggtcgtacat ttaagaaatt
    64981 aaatcatttc aacttggttg ttaacgcaac ctatattttg ttaacgctgc gttacgatgg
    65041 aaagtatgtc tgcggaaata tttttaataa ctcacccgcg cgttgtattt ttcttttttt
    65101 gttcatacca aagtattaaa acccacgtaa atacgctgtt atctacgcaa aaaatatttt
    65161 gttttctttt aaatctccgt tttccgctag caaaaaacac cagacagtca ttcttcttgc
    65221 ccgtcgctga ttgctgcgat atgtcttgcg cacaagccgt gacaaacctc gtctttttgc
    65281 agtaaaaaat tgtccacggt ggtgtggaga aaaaacaaga acgttt
//
LOCUS       DAZGXS010000010        64264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGXS010000010 DAZGXS010000000
VERSION     DAZGXS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="ACHKG5_000772"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="ACHKG5_000772"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0866936.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1496..1690)
                     /locus_tag="ACHKG5_000773"
     CDS             complement(1496..1690)
                     /locus_tag="ACHKG5_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0866937.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2044..2253
                     /gene="fumD"
                     /locus_tag="ACHKG5_000774"
     CDS             2044..2253
                     /gene="fumD"
                     /locus_tag="ACHKG5_000774"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0866938.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2709..3335
                     /gene="ydhY"
                     /locus_tag="ACHKG5_000775"
     CDS             2709..3335
                     /gene="ydhY"
                     /locus_tag="ACHKG5_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0866939.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKTIEWKDITV"
     gene            3356..5458
                     /gene="ydhV"
                     /locus_tag="ACHKG5_000776"
     CDS             3356..5458
                     /gene="ydhV"
                     /locus_tag="ACHKG5_000776"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0866940.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            5471..6109
                     /gene="ydhW"
                     /locus_tag="ACHKG5_000777"
     CDS             5471..6109
                     /gene="ydhW"
                     /locus_tag="ACHKG5_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0866941.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6173..6841
                     /gene="ydhX"
                     /locus_tag="ACHKG5_000778"
     CDS             6173..6841
                     /gene="ydhX"
                     /locus_tag="ACHKG5_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0866942.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            6838..7623
                     /gene="phsC"
                     /locus_tag="ACHKG5_000779"
     CDS             6838..7623
                     /gene="phsC"
                     /locus_tag="ACHKG5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP0866943.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7627..8439
                     /gene="ydhT"
                     /locus_tag="ACHKG5_000780"
     CDS             7627..8439
                     /gene="ydhT"
                     /locus_tag="ACHKG5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0866944.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8451..10055)
                     /gene="ydhS"
                     /locus_tag="ACHKG5_000781"
     CDS             complement(8451..10055)
                     /gene="ydhS"
                     /locus_tag="ACHKG5_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0866945.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(10181..10486)
                     /gene="ydhR"
                     /locus_tag="ACHKG5_000782"
     CDS             complement(10181..10486)
                     /gene="ydhR"
                     /locus_tag="ACHKG5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0866946.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(10594..10670)
                     /locus_tag="ACHKG5_000783"
     tRNA            complement(10594..10670)
                     /locus_tag="ACHKG5_000783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10634..10636),aa:Val,seq:gac)
     gene            complement(10675..10751)
                     /locus_tag="ACHKG5_000784"
     tRNA            complement(10675..10751)
                     /locus_tag="ACHKG5_000784"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10715..10717),aa:Val,seq:gac)
     gene            11059..12315
                     /gene="ydhQ"
                     /locus_tag="ACHKG5_000785"
     CDS             11059..12315
                     /gene="ydhQ"
                     /locus_tag="ACHKG5_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0866947.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(12356..13729)
                     /gene="mdtK"
                     /locus_tag="ACHKG5_000786"
     CDS             complement(12356..13729)
                     /gene="mdtK"
                     /locus_tag="ACHKG5_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0866948.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            13944..14585
                     /gene="ribE"
                     /locus_tag="ACHKG5_000787"
     CDS             13944..14585
                     /gene="ribE"
                     /locus_tag="ACHKG5_000787"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0866949.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(14625..15773)
                     /gene="cfa"
                     /locus_tag="ACHKG5_000788"
     CDS             complement(14625..15773)
                     /gene="cfa"
                     /locus_tag="ACHKG5_000788"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0866950.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(16064..17275)
                     /gene="punC"
                     /locus_tag="ACHKG5_000789"
     CDS             complement(16064..17275)
                     /gene="punC"
                     /locus_tag="ACHKG5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0866951.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17388..18320
                     /gene="punR"
                     /locus_tag="ACHKG5_000790"
     CDS             17388..18320
                     /gene="punR"
                     /locus_tag="ACHKG5_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0866952.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18317..19342)
                     /gene="purR"
                     /locus_tag="ACHKG5_000791"
     CDS             complement(18317..19342)
                     /gene="purR"
                     /locus_tag="ACHKG5_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0866953.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19641..19730
                     /gene="cydH"
                     /locus_tag="ACHKG5_000792"
                     /gene_synonym="ynhF"
     CDS             19641..19730
                     /gene="cydH"
                     /locus_tag="ACHKG5_000792"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0866954.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            19896..21065
                     /gene="ydhP"
                     /locus_tag="ACHKG5_000793"
     CDS             19896..21065
                     /gene="ydhP"
                     /locus_tag="ACHKG5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0866955.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(21211..21792)
                     /gene="sodB"
                     /locus_tag="ACHKG5_000794"
     CDS             complement(21211..21792)
                     /gene="sodB"
                     /locus_tag="ACHKG5_000794"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0866956.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21920..22735)
                     /gene="mepH"
                     /locus_tag="ACHKG5_000795"
     CDS             complement(21920..22735)
                     /gene="mepH"
                     /locus_tag="ACHKG5_000795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0866957.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23070..23417
                     /gene="grxD"
                     /locus_tag="ACHKG5_000796"
     CDS             23070..23417
                     /gene="grxD"
                     /locus_tag="ACHKG5_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0866958.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23468..28084)
                     /gene="lhr"
                     /locus_tag="ACHKG5_000797"
     CDS             complement(23468..28084)
                     /gene="lhr"
                     /locus_tag="ACHKG5_000797"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP0866959.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(28177..28824)
                     /gene="rnt"
                     /locus_tag="ACHKG5_000798"
     CDS             complement(28177..28824)
                     /gene="rnt"
                     /locus_tag="ACHKG5_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0866960.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(28927..29334)
                     /gene="gloA"
                     /locus_tag="ACHKG5_000799"
     CDS             complement(28927..29334)
                     /gene="gloA"
                     /locus_tag="ACHKG5_000799"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0866961.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(29415..30512)
                     /gene="nemA"
                     /locus_tag="ACHKG5_000800"
     CDS             complement(29415..30512)
                     /gene="nemA"
                     /locus_tag="ACHKG5_000800"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0866962.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VHDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(30549..31148)
                     /gene="nemR"
                     /locus_tag="ACHKG5_000801"
     CDS             complement(30549..31148)
                     /gene="nemR"
                     /locus_tag="ACHKG5_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0866963.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            31251..31490
                     /gene="ydhL"
                     /locus_tag="ACHKG5_000802"
     CDS             31251..31490
                     /gene="ydhL"
                     /locus_tag="ACHKG5_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0866964.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            31539..32435
                     /gene="ydhF"
                     /locus_tag="ACHKG5_000803"
                     /pseudo
     CDS             31539..32435
                     /gene="ydhF"
                     /locus_tag="ACHKG5_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            32516..33037
                     /gene="sodC"
                     /locus_tag="ACHKG5_000804"
     CDS             32516..33037
                     /gene="sodC"
                     /locus_tag="ACHKG5_000804"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0866965.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(33038..35049)
                     /gene="ydhK"
                     /locus_tag="ACHKG5_000805"
                     /pseudo
     CDS             complement(33038..35049)
                     /gene="ydhK"
                     /locus_tag="ACHKG5_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(35049..35906)
                     /gene="ydhJ"
                     /locus_tag="ACHKG5_000806"
     CDS             complement(35049..35906)
                     /gene="ydhJ"
                     /locus_tag="ACHKG5_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0866966.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(35909..36145)
                     /gene="ydhI"
                     /locus_tag="ACHKG5_000807"
     CDS             complement(35909..36145)
                     /gene="ydhI"
                     /locus_tag="ACHKG5_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0866967.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            36346..36780
                     /gene="slyA"
                     /locus_tag="ACHKG5_000808"
     CDS             36346..36780
                     /gene="slyA"
                     /locus_tag="ACHKG5_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0866968.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(36827..37294)
                     /gene="slyB"
                     /locus_tag="ACHKG5_000809"
     CDS             complement(36827..37294)
                     /gene="slyB"
                     /locus_tag="ACHKG5_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0866969.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            37567..38676
                     /gene="anmK"
                     /locus_tag="ACHKG5_000810"
     CDS             37567..38676
                     /gene="anmK"
                     /locus_tag="ACHKG5_000810"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0866970.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            38774..39103
                     /gene="mliC"
                     /locus_tag="ACHKG5_000811"
     CDS             38774..39103
                     /gene="mliC"
                     /locus_tag="ACHKG5_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0866971.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            39162..39818
                     /gene="pdxH"
                     /locus_tag="ACHKG5_000812"
     CDS             39162..39818
                     /gene="pdxH"
                     /locus_tag="ACHKG5_000812"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0866972.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            39947..41221
                     /gene="tyrS"
                     /locus_tag="ACHKG5_000813"
     CDS             39947..41221
                     /gene="tyrS"
                     /locus_tag="ACHKG5_000813"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0866973.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            41283..42143
                     /gene="pdxY"
                     /locus_tag="ACHKG5_000814"
     CDS             41283..42143
                     /gene="pdxY"
                     /locus_tag="ACHKG5_000814"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFP0866974.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(42187..42792)
                     /gene="gstA"
                     /locus_tag="ACHKG5_000815"
     CDS             complement(42187..42792)
                     /gene="gstA"
                     /locus_tag="ACHKG5_000815"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0866975.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(42898..44400)
                     /gene="dtpA"
                     /locus_tag="ACHKG5_000816"
     CDS             complement(42898..44400)
                     /gene="dtpA"
                     /locus_tag="ACHKG5_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0866976.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(45011..45646)
                     /gene="nth"
                     /locus_tag="ACHKG5_000817"
     CDS             complement(45011..45646)
                     /gene="nth"
                     /locus_tag="ACHKG5_000817"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0866977.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(45646..46341)
                     /gene="rsxE"
                     /locus_tag="ACHKG5_000818"
     CDS             complement(45646..46341)
                     /gene="rsxE"
                     /locus_tag="ACHKG5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0866978.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(46345..46965)
                     /gene="rsxG"
                     /locus_tag="ACHKG5_000819"
     CDS             complement(46345..46965)
                     /gene="rsxG"
                     /locus_tag="ACHKG5_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0866979.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(46969..48027)
                     /gene="rsxD"
                     /locus_tag="ACHKG5_000820"
     CDS             complement(46969..48027)
                     /gene="rsxD"
                     /locus_tag="ACHKG5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0866980.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(48028..50250)
                     /gene="rsxC"
                     /locus_tag="ACHKG5_000821"
     CDS             complement(48028..50250)
                     /gene="rsxC"
                     /locus_tag="ACHKG5_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0866981.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAITRAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED
                     "
     gene            complement(50243..50821)
                     /gene="rsxB"
                     /locus_tag="ACHKG5_000822"
     CDS             complement(50243..50821)
                     /gene="rsxB"
                     /locus_tag="ACHKG5_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0866982.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(50821..51402)
                     /gene="rsxA"
                     /locus_tag="ACHKG5_000823"
     CDS             complement(50821..51402)
                     /gene="rsxA"
                     /locus_tag="ACHKG5_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0866983.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(51479..51919)
                     /gene="ydgK"
                     /locus_tag="ACHKG5_000824"
     CDS             complement(51479..51919)
                     /gene="ydgK"
                     /locus_tag="ACHKG5_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0866984.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(52005..52220)
                     /gene="ydgT"
                     /locus_tag="ACHKG5_000825"
     CDS             complement(52005..52220)
                     /gene="ydgT"
                     /locus_tag="ACHKG5_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0866985.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(52493..52618)
                     /gene="blr"
                     /locus_tag="ACHKG5_000826"
     CDS             complement(52493..52618)
                     /gene="blr"
                     /locus_tag="ACHKG5_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0866986.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            52861..53901
                     /gene="ydgJ"
                     /locus_tag="ACHKG5_000827"
     CDS             52861..53901
                     /gene="ydgJ"
                     /locus_tag="ACHKG5_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0866987.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(53937..54938)
                     /gene="add"
                     /locus_tag="ACHKG5_000828"
     CDS             complement(53937..54938)
                     /gene="add"
                     /locus_tag="ACHKG5_000828"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0866988.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(55042..56214)
                     /gene="malY"
                     /locus_tag="ACHKG5_000829"
     CDS             complement(55042..56214)
                     /gene="malY"
                     /locus_tag="ACHKG5_000829"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0866989.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(56224..57816)
                     /gene="malX"
                     /locus_tag="ACHKG5_000830"
     CDS             complement(56224..57816)
                     /gene="malX"
                     /locus_tag="ACHKG5_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0866990.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            57991..59019
                     /gene="malI"
                     /locus_tag="ACHKG5_000831"
     CDS             57991..59019
                     /gene="malI"
                     /locus_tag="ACHKG5_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0866991.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            59131..59898
                     /gene="hdhA"
                     /locus_tag="ACHKG5_000832"
     CDS             59131..59898
                     /gene="hdhA"
                     /locus_tag="ACHKG5_000832"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0866992.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            60127..60717
                     /gene="uidR"
                     /locus_tag="ACHKG5_000833"
     CDS             60127..60717
                     /gene="uidR"
                     /locus_tag="ACHKG5_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0866993.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            61108..62921
                     /gene="uidA"
                     /locus_tag="ACHKG5_000834"
                     /pseudo
     CDS             61108..62921
                     /gene="uidA"
                     /locus_tag="ACHKG5_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            62918..>64264
                     /gene="uidB"
                     /locus_tag="ACHKG5_000835"
     CDS             62918..>64264
                     /gene="uidB"
                     /locus_tag="ACHKG5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0866994.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQ"
BASE COUNT        16180 a        15848 c        16694 g        15542 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc tctgcagagc
      181 cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgggtagc
      361 aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag cgacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa acgccaggca ggttcacacc
     1021 tttgttttca cccaggtcgc cgttgttcag cactttacag ataactttgt taccttcaat
     1081 ggcagtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caacagacag
     1141 gtcagttgtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtagtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1741 aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat
     1801 tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc tgtttgcgca
     1861 acgtctgtgc cggggttgtc actgtgaaga taagtgaaag ttcatccggt gtataaatac
     1921 atcatcgctc aattgctaca gttaaaataa tttatcgcgt tatttgtctg attttgtttg
     1981 atttttgttt aattggttag attgatatat gctgatagtt ggcatgataa ataggtataa
     2041 agaatgggaa atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt
     2101 aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc
     2161 gtattacgta cagcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg
     2221 atggaaaccg tcattaacgc actggtaaaa tgaccattca ggatgaggcg ataagcttca
     2281 tcctgatatt cttgcttcag ttgcggtcca gttaatcatt ctaaataaca actgctgaaa
     2341 ttatacgaat ttcgatatgt gttttaattt ataacgataa tattattttt attttctgca
     2401 atgaccgtca caaataattg tcgcttaagt taaattcatt tcaaatgata ataaatatca
     2461 gttcacttat aatttcttgc tttgtaattt taattgataa tgatcaatgt taagagccca
     2521 gaataaccac attatttttt ctcctaacca taaaggatta gtttatcggc gttaaatatt
     2581 ttctcataca ctatcgggtt agggaaaata tctggtgttg gggacagagt cagcagcgta
     2641 ttagttgaat gcttaatatc aggctgctga attgtcagca ctgtgtttgc gaagaggatg
     2701 cgaaataaat gaacccggtt gatcgtccac tattagatat tggcttaacg cgattagaat
     2761 tcttgcgtat atcaggaaaa ggccttgctg gtctaacaat tgcccctgcg ttactgtcac
     2821 ttttgggttg caaacaggaa gatattgata gcggcacagt agggttgata aatacaccca
     2881 aaggggtgct ggtgacccag cgagcgcgat gcacgggttg ccaccgctgt gaaatctctt
     2941 gtaccaactt caacgatggc tcagtaggga cattcttctc ccgtatcaaa atccatcgca
     3001 attatttctt tggcgacaac ggggttggct ctggcggcgg cctgtatggc gatctcaact
     3061 ataccgcgga cacctgccgt caatgcaaag agccgcaatg catgaacgtc tgcccgattg
     3121 gtgcgattac ctggcagcag aaagaaggct gtattaccgt cgatcataaa cgttgtattg
     3181 gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc caccgtaaat accgaaagta
     3241 aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca acaggggcgt
     3301 taaaaactat cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa aaatcatgac
     3361 taacggttgg acaggtaata tattaagagt caatctcacg acaggaaata ttaccctcga
     3421 agattccagt aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca aaattatgta
     3481 tgacgaagta ccgccaggca cgaaaccttt cgatgaagcg aataaattag tctttgctac
     3541 cggcccatta actggatctg gtgccccctg tagttctcgc gtaaatatca cctcactttc
     3601 tacttttacc aaaggaaatt tagtcgtcga tgcccatatg ggtggctttt ttgcagcgca
     3661 aatgaaattc gctggatacg acgtcattat tatcgaaggg aaggcgaaat caccggtatg
     3721 gctgaagatt aaagatgaca aagttagcct ggaaaaagca gatttcttat ggggaaaagg
     3781 gacgcgcgca acgacggaag aaatttgtcg cttgaccagt ccggaaacct gtgtggcggc
     3841 tattggtcag gctggggaaa accttgttcc tctctctggc atgttgaata gccgtaacca
     3901 cagcggcggt gcgggaactg gcgcaataat gggttcgaaa aacctgaaag cgattgcggt
     3961 tgaagggacg aaaggggtca acattgccga tcgtcaggag atgaagcgtc tcaatgatta
     4021 catgatgact gaacttatcg gtgcgaataa caaccatgtc gtgccaagta cgccgcaatc
     4081 gtgggcagag tattcagatc ccaagtcacg ctggacagca cgtaaagggc tgttttgggg
     4141 cgcggctgaa ggcggtccaa ttgaaacggg tgaaattccg ccaggcaatc agaatacggt
     4201 cggctttcgt acctataaat ccgtttttga cttaggtccg gcagcagaga aatacacagt
     4261 aaaaatgagc ggctgccact cttgcccgat ccgttgtatg acccaaatga atattcctcg
     4321 ggtgaaagag tttggcgtgc ccagcacagg tggtaacact tgtgtagcaa actttgtcca
     4381 taccaccatc ttcccgaacg ggccgaaaga ttttgaagat aaagacgatg gtcgtgtgat
     4441 tggtaacctg gttggtctga atttgttcga tgactacggc ctatggtgta actacgggca
     4501 gttgcatcgc gactttactt attgttacag caaaggtgtg ttcaagcgtg ttctgcctgc
     4561 tgaagagtat gcagaaattc gctgggatca actggaagcg ggagacgtta acttcattaa
     4621 agatttttac taccgtctgg cgcatcgtgt gggtgagctg agtcacctgg ctgatggttc
     4681 atatgccatc gcagaatgct ggaatttggg tgaagagtac tggggctacg cgaaaaataa
     4741 actctggtcg ccgtttggct atccggttca ccatgccaat gaagcgtcag cgcaggtcgg
     4801 ttccattgtt aactgtatgt tcaaccgtga ctgcatgacg catacccata tcaactttat
     4861 tggttccggc ttgccattga aactgcaacg tgaagtggcg aaagaacttt ttggttctga
     4921 agatgcttac gatgaaacca aaaactacac gccaatcaat gacgcaaaaa ttaaatatgc
     4981 taagtggtcg ctgttgcggg tctgtttgca taacgccgtc accctgtgca actgggtctg
     5041 gccaatgacc gtttcgccgc tgaaaagccg taattaccgg ggcgatctgg cgcttgaagc
     5101 caaattcttc aaagcgatca ctggcgaaga gatgactcag gaaaaattag atttagctgc
     5161 agagcgtatc tttacattgc atcgtgccta cacggtgaaa ctgatgcaaa ccaaagatat
     5221 gcgtaatgaa cacgatctta tctgttcctg ggtattcgac aaggatccgc agatcccggt
     5281 ctttactgaa ggtactgaca aaatggatcg tgatgatatg catgcctcgc tcacgatgtt
     5341 ctacaaagaa atgggctggg acccacagct tggttgtccg acccgcgaaa cattgcagcg
     5401 tctggggctg gaagatattg ccgccgatct ggcagcacac aatctactgc ctgcgtaagg
     5461 aatgggtaag atgaaccatc aggatgaatt gccgttagcg aaagtcagcg aagttgacga
     5521 ggcgaagcgg caatggttgc aggggatgcg tcaccccgtt gatacggtaa ccgaaccaga
     5581 accggctgaa atcctggcag agttcattcg ccagcattct gtggctgggc agctggtggc
     5641 gcgcgccgtg tttctctcgc caccctattc agttgcagaa gaggaactgt cagttttgct
     5701 ggaaaacatc aagcagaacg gagactacgc ggatatcgct tgtatgacgg gaagtcagga
     5761 tgattactat tactcaacgc aggccatgag tgaaaactac gcggcgatgt ctttgcaagt
     5821 tgtggagcag gacatttgtc gcgccattgc acacgccgtt cgttttgagt gccagacgta
     5881 tcctcgtcct tacaaagtcg ccatgctgat gcaagcgcca tattacttcc aggaagcgca
     5941 gattgaagcc gcgatcgccg caatggatgt tgcacctgaa tatgccgata tacggcaggt
     6001 agagtcatcg acggcggtgt tgtacttgtt tagcgagcga tttatgacct atggcaaggc
     6061 ttatggctta tgcgaatggt tcgaggtaga gcagtttcag aacccctgaa atttcaggga
     6121 gatgtcatgg atagggtgga cagttgcagc cactgcatta ggggataatc agatgtcctt
     6181 cactcgacgc aaatttgttc tggggatggg aacagtaatc ttttttactg gttctgcgtc
     6241 ttctctgtta gcgaacacga ggcaagaaaa ggaagttcgg tatgccatga ttcatgacga
     6301 gtcacggtgc aatggctgta atatttgcgc ccgcgcatgc cgtaaaacta accacgcccc
     6361 cgctcaggga agccgtttat cgatagcaca cattccagtc accgataacg acaacgaaac
     6421 gcagtatcac ttttttcgcc agtcatgtca gcactgcgaa gatgctccgt gcatcgacgt
     6481 atgcccgaca ggggcgtcgt ggcgtgatga gcaggggatc gtgcgggtag aaaaatcgca
     6541 gtgcatcggc tgcagttatt gcattggcgc ttgtccgtac caggttcgct accttaaccc
     6601 tgtcactaaa gtggcagata aatgtgattt ctgcgctgag tcccgactgg caaaaggctt
     6661 tccgcccatt tgcgtcagcg cttgcccgga acacgcattg atctttggtc gcgaagatag
     6721 ccctgaaatt caggcgtggt tgcaggataa taaatactat caatatcagc tacctggtgc
     6781 cgggaaaccg catctgtatc gccggttcgg tcaacatttg attaaaaagg aaaatgtatg
     6841 aacccgtcgc aacatgctga acagtttcag agccagttag cgaactatgt gccacagttc
     6901 actcccgaat tttggccggt gtggttgatc attgccggag tattgctggt tgggatgtgg
     6961 ttggtgctgg ggctgcacgc cttgcttcgt gctcgtggcg tgaagaaatc agccaccgat
     7021 catggtgaga ggatttatct ttacagcaaa gcggtcagat tatggcactg gtcgaatgcg
     7081 ttactctttg tattgttgct ggcaagtggg ctgataaatc actttgcgct ggtgggcgca
     7141 actgcggtta aaagtctggt tgcggtgcat gaagtttgcg gatttttgtt actggcatgc
     7201 tggctcggct ttgttctgat caatggcgtt gggggtaatg gccaccacta tcgcattcgt
     7261 cgtcagggtt ggctggagcg ggcggcaaaa caaacgcgat tttatttgtt tggcattatg
     7321 cagggggaag aacatccttt cccggcaaca acccagtcta aatttaatcc cttacagcag
     7381 gttgcctatg ttggtgtcat gtatggattg ctgccgttgt tactattgac ggggctgctg
     7441 tgtctctatc cgcaagccgt gggagatgtg tttcctggcg taagatactg gttattgcag
     7501 gcgcattttg ctctggcatt tataagcctc ttttttatct tcggtcatct ttatctttgc
     7561 accacggggc gtacgccaca cgaaaccttt aaaagcatgg tcgatggcta tcaccggcac
     7621 taacgaatga tcattacacg agcagacctg cgagaatggc gtatcggagc ggtgatgtat
     7681 cgctggttcc tgcgccattt cccacgcggc ggtagttatg ccgatattca tcatgcactg
     7741 attgaggaag gatatacgga ctgggcggag agtctggttg agtatgcgtg gaaaaaatgg
     7801 ctggcggatg aaaacttcgc gcatcaggaa gtttcctcta tgcaaaaact ggccacagac
     7861 cccggagaga gagcattttg cagtcagttt gcccgcagcg acgatcatgc tcgcatcggc
     7921 tgttgtgagg ataacgccag aatcgcaacc gctgggtatg ctgcacaaat tgccagtatg
     7981 ggttatagcg tgcggattgg aagtgtcggc tttaacagtc acattggcag ctccggtgag
     8041 cgcgcacggg ttgctgtaac cggcaattct tcgcggataa gcagcgctgg agatagcagt
     8101 cggattgcga ataccggaat gcgagtacgc gtttgtacat tgggtaaacg gtgtcatgtc
     8161 gccagtaatg gtgacctggt gcaaatcgcc agctttggcg caaacgccag aattgccaac
     8221 agcggcgaca atgtacacat cattgccagc ggtgaaaatt caacggtagt gagcacgggc
     8281 gtggtcgact caattattct cgggccaggg ggcagcgccg cactggcata tcatgacggc
     8341 gaaagagttc gcttcgccgt cgccattgaa ggtgaaaaca atattcgagc cggagtccgc
     8401 tatcgtctta atgaacagca tcagtttgtt gagtgctgat agctttacct ttaatcaaac
     8461 gaaagacgcc gacgagcacg ggatgcaggc tttacgaccg cccgagccat cgcctcacca
     8521 atttctgcac atgccgtaag tccctgaacg aaaggctggt cgtgcatcaa ccagggcaac
     8581 gcgccgaacg ctacgcgccc acgaatatct tcgggttgct gtaacgtata atcttcacca
     8641 acatcaggga tttcatcccc tgttttctgt aattgttcgc gtagtccagg gaaaggaata
     8701 tctttcactt taagcggacg ctgcccgcgg gcatcaataa aaacgtcaaa cgagtagctg
     8761 ttgtcttccg ttttcaggac ggtgcgcgac tcgttaattt ccattttgta gtcttcaccg
     8821 agagcgagaa tatgaatgat tcccgcttca cgtaaggcaa gtaggcgacg aatagactct
     8881 gaagggattg ccgcataatt atcgatgaaa acccgggcaa ggcctttact gaaccgttta
     8941 tggtcgtgtt cattcagatg tggaacaatt tcctgtacgg cttcatgcag gcgcagaatg
     9001 acataacgcc agggaacagt atgtttttct cgtttattgc gttcgacttc ctggagattt
     9061 ttttctgccc agtcaaattg gtcgcgttgt ttgcgctcgg caaaccaggc ttgagcaaag
     9121 gaatcgacat tcaggctctc taaggctatg cgttgactcc agtctggatc agcaaacttg
     9181 atttcctcta ctatcaatct aaatacccga tccaaaaggc catattcgcc tttttgaatc
     9241 tcagcattta atgcctgatc agtgacgatg tgtaagggct cgtagggaat agggcaatag
     9301 aaatcggctt cgggtaaaat acccgtgcgc gacattaacg taatatttag cttttcactt
     9361 gcgttatcgc ggtgaaaaac gacgtgttgt ttatcatctt caatgaacga accatgctga
     9421 atagccactg ccattgccgc atccagtccg ctcaaggatg ttcccataat acccacgtta
     9481 cacgcatcga cctttgcttc catcaagcct gaccacgggc ttggaaaata cgttcgggtt
     9541 gcttcttctt catcaggcca gacgtgaccc gtggcgatca ccgctaaatc aaacgtctcg
     9601 ctgggtaaat cctgatttgt agcgagcatg acgccagcat ttgtaatttg cagatcggta
     9661 acctggcatg attcataaac agccactgca aatttttgct ttcgtgcctg gtctactaat
     9721 cgtaaaaatt gatcgcggaa atattcgccc agcagaattc gcggtaaaaa ctgacgatcg
     9781 tgcaaggttt cttttttaac gccataacgc tggagatgac tggcttcttg cttttgtagc
     9841 cattcgagat acgtacaata aatcggcggt atttcaatac tggcaatatt tgccagcatc
     9901 atttttgagt tttcctcatc actgtatggc atcccgacac cggcctcgtc agcctgctcg
     9961 aagatagaaa tagaaagtgg agtttgttgc tgtagaagcg agaataaggt gtagatcccc
    10021 gtaggcccgg cacccacaat tgcaattttt ttcattgtta cttacctgta cgcattcttt
    10081 cgaatttagt gttatcagat aagtttggtt gagattttta gaatttgaga aatttcagac
    10141 agggatgaaa agggcgctaa ccgcccttga ttaaattctg tcaggcgagt tttgcctgat
    10201 tgatttgact cagtggctca ttgacatcga aaactttggc gacaacttcc tcaacgccga
    10261 gttttttcag ttgggcagtg tgtttttcca gataggcaag ggcgcttttt tcatcagtga
    10321 aaaggtagat cccaccggct tcgtggttct tttcactttc tgtccatacc ttccacagaa
    10381 aaccaggttc ctgattaatc gactctgcaa gtggtttaag ctgctcagcc attgcgtcgc
    10441 caaatgggcc attaaaagca aaatgaagtt gtaacaaagt tgccatagcg attctccttc
    10501 tgataacgca tacgaagaca gtgtaaatct tttaacgtat tggatacaga gaagaagcag
    10561 agggaaaaag gagaagacca gattaagaaa atctggtgcg tccgagtgga ctcgaaccac
    10621 cgacccccac catgtcaagg tggtgctcta accaactgag ctacggacgc aggatggtgc
    10681 gttcaattgg actcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga
    10741 gctatgaacg caacgttgta ggtgacaacg gggacgaata ttagcggcag agtgggaagg
    10801 tggcaagagg caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag cttttaagca
    10861 aattggctat attttgttga tttgcaaggg cgatttttat tcaggatcgc atttacatct
    10921 gatacaaccc aataattcgt gtatcagagt ttaaacagag ttgtttgcca ccatctcaaa
    10981 tctgtcgtgg ataaatcctc cgtagccttt acacttaggt agcaataaag gcacacagat
    11041 gcagcaaagg aataaatcat gggatctgat gcgaaaaact tgatgagcga cgggaatgtg
    11101 caaattgtta agaccggcga ggtcattggc gcgacgcaac ttactgaagg cgagttgatt
    11161 gttgaagctg gcggaagagc cgaaaatacc gtggtcacgg gggctggctg gttgaaagtg
    11221 gcaaccggtg ggatcgccaa atgcacacag tacggtaaca atggcacgct atcggtcagc
    11281 gacggtgcca ttgccacaga tattgttcag tccgagggag gcgcaattag tctctctacg
    11341 ctcgctacgg ttaatggccg ccatcccgaa ggtgaattca gcgttgatca gggttatgcc
    11401 tgcggtttgt tgctggaaaa tggcggtaac ctgcgtgtac tggaaggaca tcgcgcggaa
    11461 aaaatcattc tcgatcaaga gggcggcctg ttggttaatg ggacaacctc agcggtcgtg
    11521 gtagatgaag gtggtgaatt gttggtgtat ccaggtgggg aagccagcaa ttgtgagatt
    11581 aatcagggcg gcgtttttat gctggcgggg aaagccaatg atacgttgct tgctgatggc
    11641 accatgaata atctcggtgg tgaagactct gacactattg ttgagaatgg agccatctat
    11701 cgtctgggga cggatggtct tcagctctac agttccggta agacgcaaaa cctgtccgtt
    11761 aatgtgggtg gtcgggctga agtgcatgcc ggtacgctgg aaaatgcggt aatacaaggt
    11821 ggaacagtga tcctgttgtc acccaccagc gcggacgaaa attttgtcgt agaggaagat
    11881 cgcgcaccgg ttgaactgac cgggagtgtt gcattactgg acggcgcttc aatgattatt
    11941 ggctatggcg cagatctgca acaatcaacg attactgtac agcagggcgg tgtattgatt
    12001 ctcgacggca gtacggtaaa aggtgacagt gtcactttca gtattggtaa catcaatctg
    12061 aatggcggaa aactgtggct gatcactggt gcggcaacgc atgtgcagct gaaagtgaaa
    12121 cgcctgcgcg gagagggagc gatttgcctg caaaccagtg cgaaagaaat ttcacctgac
    12181 ttcatcaatg tgaaagggga agttaacggg gatatacgcg ttcagataac agatgccagt
    12241 cggcaaactc tgtgtaacgc actgaaacta cagccagacg aagacgggat tggcgcaacg
    12301 ctccagcctg cgtaaaaaca caggcgcacc ccgtgaaggt tgcgccttgt cttcattagc
    12361 gggatgctcg ttgcagaatg atggctgacg gcagacgttg caggtaccgc atacgcagca
    12421 tcatcataat ggctgccgac gtcaggccaa taataaagcc tatccagaag cctgctggcc
    12481 ccataggttc aacgaccaga tcggtcagtg ccagaatata gccgcttggc aagcccagca
    12541 cccagtaagc cgtaaaggtg atatagaaaa tggaacgcgt atctttataa ccacgcaaaa
    12601 tcccactgcc aatcacctgg attgagtcag aaatctgata taccgccgcc agcaacatca
    12661 aatgcgccgc cagcgttaca acctcgggat tgtcgttgta caacagggcg atttgctccc
    12721 gcagtgaaac cgtgaatatg gccgtcaggg ttgccataca gacacccacc ataagcccgg
    12781 tccgcgcagc ggtttgcgca tccagcgttg agccctgacc cagacgataa cctacgcgga
    12841 tagttaccgc tgccgccagc gacattggaa gcacgaacat tagtgaacta aagttcaggg
    12901 caatctggtg tcctgcgaca tcaacaatac cgagcggaga cactaacaga gccacgacgg
    12961 caaacagtgt cacttcaaag aatagtgcca gcgcaatcgg caaaccgagt tgaatcagtc
    13021 gtttcataac cgcaggatcg ggtttcgcgg tgcctttttc gttacgaata tcgcgcatcg
    13081 agcgggcgcg tttaatgtaa gaaaccatgg caaggaacat gacccaatac accgccgcag
    13141 tagccacgcc acaaccaacg ccaccgagct caggcatacc gaaatgacca taaataaaga
    13201 tatagttcac cggaatgttc accagcaggc cgataaagcc cattaccata cccggcttgg
    13261 tttttgccag accttcacat tggttacggg caacctggaa gaaaagatag cccggcgcgc
    13321 cccacagcaa cgcacgcaga taacccacgg ctttgtccgc cagagccgga tcgatgtttt
    13381 ccatggagcg gataatgtaa cctgcattcc acagcaccag cataatgaga acggaaacaa
    13441 aacccgccag ccagaagccc tgtcgcacct gatgcgcaat gcgctcacgt cgaccggaac
    13501 catttaattg cgcgataacc ggcgttaatg ccagcagcag tccgtgacca aagaggatcg
    13561 ccggaagcca gatagaagta ccgatagcga ccgccgccat gtcggtggca ctatagccgc
    13621 ccgccatcac ggtatcgaca aaacccatcg cagtttgggc gatttgcgcg agaatcaccg
    13681 ggattgctaa tgctaataac agacgcgctt cactgatata cttctgcacg tgaacacctt
    13741 ttatttgtag ttatatgaga gactaaaaag ccgcctgaat aggcagcaaa aagaagaagc
    13801 aggggaaatt cagtctattg tagcgaggat ttattaattc tccagtgaaa aaatagccat
    13861 tcttacccct tcactggtaa gggcgttttc cacctgctat tgtgctgaac agaatgtctt
    13921 aactgatttc aggagttgta agtatgttta cggggattgt acagggcacc gcaaaactgg
    13981 tgtcgattga cgagaaacca aattttcgca cacatgtggt ggagttaccc gaccacatgc
    14041 tggacggtct ggaaaccggt gcctccgtgg cgcataacgg ttgctgcctg accgtgacgg
    14101 aaattaacgg caaccatgtc agttttgacc tgatgaaaga aacgttgcgc attaccaatc
    14161 ttggcgattt aaaagtgggg gattgggtaa acgttgagcg tgcggcgaaa ttcagtgatg
    14221 aaattggcgg acacttaatg tcaggtcata ttatgaccac cgctgaagtg gcgaaaatat
    14281 taacctcgga aaataatcgc cagatctggt ttaaagtcca ggatagtcag ttgatgaaat
    14341 atatcctgta caaaggattt attggcatcg acggtattag cctgaccgtc ggcgaagtca
    14401 cgccaacgcg tttttgcgtc catttaattc cggaaactct ggaacgcacg actcttggga
    14461 agaaaaaact tggcgcacgc gtcaacattg aaatcgatcc acaaactcag gcagtggtag
    14521 atacggtaga acgtgtgctg gcggcacgag aaaatgccat gaatcaacca ggtacagaag
    14581 cctgatctga aatcgccccc ggagaggggg cgatagaata gcctttagcg agcgactcga
    14641 aggccgtttt caacaccgcg tgagaacacc acctgccaga gctgaatatc acgggcgcgg
    14701 aaagcacctg cgcaggcatt cagataatag gtaaacatcc gtttaaagcg ttcactgtag
    14761 ttatccgcaa tttctggcca tgcggcgacg aatcgttcat accacgccat caacgtagta
    14821 tcgtaatcag caccgaagtt atgccagtct tccatcacaa agtggggttc gctggactga
    14881 gcaatctggc gtacagaggg caggcaaccg ttcgggaaaa tatatttatt aatccaggga
    14941 tcaacattca gatcggtttt tttcgaaccg atagtatgga gcaggaatat gccttccggt
    15001 ttcaaattac gatccaccac cgtaaaatag gtatcgtaat ttttcggtcc gacgtgctcg
    15061 aacatcccca cagaaacaat acgatcaaac tggtcgttca ggtcacgata atcttgcagc
    15121 aaaatggtga catccaggcc ttcacagcgt tcctgagcca ttttttgctg ttcggcagaa
    15181 atggtgacgc ccaccacgct tacgtcataa ttagatgcca tgtagtgtgc cagtccgccc
    15241 cagccgcagc caatatccag tacgcgcatc ccaggtttta actgcaattt ttcacaaatc
    15301 attttgagct tcgcctgctg ggcagattcc agattatcgg catctttcca gtaagcgcag
    15361 gaatattgca tgaagggatc aagcatgcgg ctgaacaagt cattacccaa atcgtaatgc
    15421 tctttgccga ctatccaggc acgtttttta ctctgcagat tgaagagacg ggcgccggca
    15481 atacgcagcg tgtctttgaa atgatggggg agttggttct cgagacctgc gcgtaagact
    15541 ttgctaaaaa acatatccag tcggtcacat tcccaccagc catccatata actttcgcct
    15601 aaccccaaag agccttcttg cagaacgcgt ttaaaaaaat cggggttttt cacacgaata
    15661 tccgccgggg cagaaccgtt aatggctata ccggcacggc taagtaattc gttggcgata
    15721 cggtaccagt tgtcatccgg tacactgact tcttctatac acgatgaact catagtttct
    15781 ccatcactgg tcggtgatca gaaccttaaa acagcgtaga cgctttttgg gctttgtgag
    15841 aaatctcgca gaaaaaaccg cgagtctata atccgacgta gaacagaggg aaaggaggaa
    15901 cccttgccga aatgaccatc catagtgagt cgggaacgat cctgtccctg taaaaacata
    15961 atatttatcg tagctttaac aactcgaaat tagtataggc cgctaatttc aatcatacaa
    16021 caaaattttg ttagcagcct aagtataagt atatcgatat agatcagtgt gattcgctat
    16081 gagcgacttc ggcattgcca tgattctggc agccaacttc ttcacaacgt tgcatcatgt
    16141 aacccagcgc gaccagcact actgttgata acatcacgct ggtggtggtg agcaatggcg
    16201 tgctgatact gatcagccag gaaactacca gacttgcgag gaagcacaga cccagttgca
    16261 gagtgttctg caacgctgcg gcgcgaccag ttgcgtgtgg gaagggacgc agagcctggg
    16321 cgacaacaat agggtagatc gcgccattag caatcgccat cacacagaat gggatcagga
    16381 tttcgaccag cgacacatgg ctaatgaagc ccgcagccca ggtcgcaatg acgctgacag
    16441 caaacagcac cagcaaccac ggtaataact gcttgccttg ccatttctgc agcgcggcgc
    16501 gacagccata accaccaatc agaaacgcga tagtttgcgg gacataactt aaaccaataa
    16561 ctgccgggct gtagcccatt tcactaagga tgaacggtga accggtcagc catgcaaaaa
    16621 aactggctga acaggccgcg tatatcagca cattgccgcg ataggtttta gaacgtagca
    16681 ggtcggtaaa ggtcagacca tcctgactat tgttacgggc cttcgtcgtg ggtttgagcc
    16741 agaaaatagg cagaatcagc accacggtaa tggcaaaaag ggtggcgaaa atcgcctgcc
    16801 aggaaaaatg gaccagcagc cagcttccta acagaggagc cagggccgga gatagaccca
    16861 ccagcggcat gatggtcgca aaaattcggt taactttctg tgaaggataa taatccgtca
    16921 ctaacgcttg ccagataacc gccgcggcgc agacacccac agcctgtaca aaacgcaata
    16981 ccagcagcat agcggcgttt tctacccaca gcatccccag actacctaac gcaaaaattg
    17041 tcaggccgat taataatacc ggtttacgac cataacgatc ggagagcggc ccccacagaa
    17101 gctgggctgc ggcaaaaccg gcaaggaaca gactaaggct ggcactgaca gcagacgcag
    17161 gcgtttgcag gtcggcctgt atggcggcga aagcaggcag atacatatcg gttgccagaa
    17221 aaccgagtac gctcaaaccc gccagccaga ctaaaaatct tttcccaggt tgcatgtata
    17281 ttctctaaat ctctcaatta aacaccgccg caaagtgtag ggagtgaaat tagtgttgtg
    17341 aaacggtaat atttgatgac tggtttcaaa aattttgcag gcagactatg tggtcagaat
    17401 attcactcga agttgttgat gcggtagcgc gtaatggtag ttttagcgct gcggcacagg
    17461 agctgcatcg cgttccttct gcggtcagct ataccgtgcg tcagctggaa gagtggctgg
    17521 cggtgccgct gtttgaacga cggcaccgtg atgtggagct gaccgctgcc ggagcgtggt
    17581 ttctcaaaga agggcgctct gttgtcaaaa aaatgcagat cacccgccag caatgtcagc
    17641 agatagcgaa cggctggcgc ggtcagttag ctatcgcagt ggataatatt gtcaggccag
    17701 aacgtacacg gcagatgatc gttgattttt atcgccattt tgatgatgtc gaacttcttg
    17761 tctttcagga agtgttcaac ggtgtctggg atgcgctttc cgacgggcgc gtggaactgg
    17821 cgattggcgc aacacgcgcg attccggtag gcggtcgtta tgccttccgg gatatgggga
    17881 tgctaagctg gagttgcgtt gttgccagcc atcacccgct ggcgttgatg gatggcccgt
    17941 tcagcgatga tacgttgcgc aactggccgt cgttggtgcg cgaagacacc tcgcgaacgc
    18001 tacctaagcg tattacctgg ttgctggata accaaaaaag agttgtcgtg cctgactggg
    18061 aatcatcggc aacctgtatc tcggcaggat tatgcatagg gatggtgcca acacatttcg
    18121 ccaaaccgtg gcttaatgag gggaagtggg tagcattaga actggaaaac ccctttccgg
    18181 attcggcatg ttgtctgaca tggcagcaaa atgatatgtc gcctgcgtta acctggttgc
    18241 ttgagtatct cggcgatagt gagacgctga ataaggagtg gctgcgggag ccggaagaga
    18301 ctcccgcaac gggtgattaa cgacgatagt cgcggaacgg gccgtcagcc acggagcggc
    18361 gttcaatcaa gcgcggatgc acttcaatgg actgcggttc ttcacgtttg ttgactatac
    18421 gatccaacaa catgttgaac gctgtttcac ccaacgaatc ttttggctga tggattgttg
    18481 tcagcgccgg cgtaaaatag cgggcgttgc gcacgttatc ataaccgatc agcgaaacat
    18541 cctgcgggac gcgcagcccc atttcatcag cagcacaaag tgcgcccatt gccatgatat
    18601 cgccaccaca gaagacggca gtagggcgat gcggctgcga caggatttgc tgcatggcgc
    18661 gataaccgga ttcaggttca aagtcaccct gcacaatcca gctttccggc accttgatca
    18721 ttgcttcttc catcgccttc ataaaaccgg caaggcggcc tgcgccagtg ttacgttcca
    18781 gcgggccggg gataacgccg atttcgcggt gaccgcgttc aatcagataa cgcccggcca
    18841 tgtagccgcc ttcgaacgcg ttatcaatga ccgcatcggt gaagtcagct tttgcttcac
    18901 cccagtccat caccaccatt gggatatggc gatactcttc cagcatcgcc agcaacggct
    18961 ctgggtactc agaacacatc accagcagac catcgacgcg tttttgcgcc atcatcgaca
    19021 gataagcccg ctgtttctca agattgttcc acgcattacc cagaatcagg gtgtaacctt
    19081 tctggaagca atttttttca actgcttcaa tgatctcggc aaaataggcc gcttcgctgc
    19141 tggtcgctag caaaccgata gacttggtgt ggttaacctt caggctacgc gccaccgcgc
    19201 taggggagta gtgtaattct ttgatcgctg cccacacggc gttgcgcgtt tcttcagcga
    19261 cgaaacgtgt tttgttgatc acgtgtgaca cagttgtagt ggaaacgttt gctcgtttcg
    19321 ctacatcttt tattgttgcc attccatttc actccagacc ctaacttcag ctcctgcaaa
    19381 atcgcaaggt aaacgtttgc ctttacacac cttacgcaca acaaaagtgt tgcggtacgc
    19441 cggaaaaacg acacggagcg tcaggaaggg gtcaatggcc agtacgcaaa tagtgtaagc
    19501 gtggaatttt ggccgatctt gacgaaaagg ggaagtggta aaaacactta tcattataaa
    19561 tgacgggaga ttttttcatc acagtgtgta aaaatgcgta atatcacttt ttgtgtggta
    19621 ataaaaaagg agaaaacttg atgagcaccg accttaaatt ttcactggta acaacgatta
    19681 tcgtcctcgg tttgatcgtg gccgtgggtt tgactgccgc gctgcactga tttcctctcg
    19741 agggagcgcg ttctccctcg catctccttc ctgtttgtta gcaaaatggc aaaagtgttt
    19801 tctctttttg ttattttcat ttttttgtga ttgctgtcat gctttcgcca ctttaatgaa
    19861 tatcgcgtaa tgacgcgaag aggtggagat aaggcatgaa aattaactat ccgttgctgg
    19921 cgctggcgat tggcgcgttt ggtatcggga caacggagtt ctcgccaatg ggcttgttgc
    19981 ccgtcattgc gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta atcagtgcct
    20041 atgcagttgg cgtaatggtt ggcgcgccgc tgatgacgct tctactttct catcgtgccc
    20101 gccgcagtgc gttgattttc ctgatggcaa ttttcacgct cggcaacgta ctttccgcca
    20161 tcgcgccgga ttatatgacc ctgatgcttt cacgcatttt gaccagcctg aatcacggag
    20221 cattttttgg tttgggttca gtcgtggccg caagcgtggt gccaaaacat aaacaggcca
    20281 gcgcagttgc cactatgttt atggggttaa ccctggcaaa tatcggtggc gtgccggcgg
    20341 cgacctggtt gggtgaaacc atcggctggc ggatgtcatt tctggcaacg gcggggctgg
    20401 gagtgatttc aatggtaagt ctgttcttct cattacctaa aggtggtgca ggggcacgac
    20461 ctgaagtgaa aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct gcattgctga
    20521 cgacggtact gggagctggt gcaatgttta ctctctacac ctatatctct ccggtactgc
    20581 aaagtattac ccacgcaaca ccggtgttcg tcacggcaat gctggtgctg attggtgtcg
    20641 gattctctat cggtaactat ctcggcggca aactggcaga tcgttcagtt aacggcacgt
    20701 tgaaaggctt tttgttgctg ctgatggtga ttatgctggc aatcccgttc ctggcccgca
    20761 ataagttcgg cgcagctatt agcatggcgg tgtggggcgc tgcaaccttt gcggtcgtac
    20821 cgccgttaca gatgcgcgtg atgcgtgtcg ccagtgaagc gccaggtctg tcttcatcag
    20881 tcaatattgg tgcctttaat cttggaaatg cgctgggagc agctgctggt ggtgcggtaa
    20941 tttccgctgg gctgggatac agctttgtgc cggtgatggg ggcgattgtc gcgggactgg
    21001 cattattgct ggtgtttatg tcagccagaa aacaacctga aacagtttgc gttgctaaca
    21061 gctaattaaa caaaaacgca gaaggataac cttctgcgtt acagttgctg acaacgtgcg
    21121 atttatcttg ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta agcgaagcgc
    21181 atcaggcaat actgcatttg ccatcagtta ttatgcagcg agatttttcg ctacgaattc
    21241 ccagttcacc agcgcccaga agtgctcaag atagccagga cgtgcattgc gatagtcgat
    21301 gtaatatgcg tgttcccaga catcaacggt tagcagcgga gtcgcatcgg tggtcagcgg
    21361 agtacccgcg ttagaggttg aaacgatagc cagtttgcca tcgctgtttt tcaccagcca
    21421 ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta aactgagctt tgaaatcggc
    21481 aaagctgcca aaagatgcgg cgatagcttc agcgactttt ccagtcggtt cgccaccggc
    21541 gttcggtgcc aggcagttcc agtagaaagt atggttccag acctgagctg cgttgttgaa
    21601 tacgccacct tcagagctgc gaataatctc ttccagtgat ttaccttcaa acgcggtacc
    21661 tttaatcagg ttgttcaggt tagtgacata agtctgatga tgcttgccgt agtgatactc
    21721 gatagtttcc gcagaaatgt gcggtgccag agcatcttta gcatatggta gtgcaggtaa
    21781 ttcgaatgac attgctactc tcctttatta ttaatttgca tacgtacaat agccttattg
    21841 tgcgtatgga tagggtagca aattaaaagg tgagacaaaa gatgaactta ccctgttgcc
    21901 gtaacaacag ggtaaagttt tagcgaagtg tttttggggt cattacccgg cgagcgccaa
    21961 catagtggcg ctgccagtag tcttcactga gagaagtgat ttgaatttcc tgacctgtgc
    22021 gcggtgactg aataaatttg ccgttgccga catacacgcc gacatgatcg gctgtaccgc
    22081 gtccctgagt acggaaaaag accaggtcgc cgtttttcag ttcactacgt tcgattggcg
    22141 ctgcatcgcg caggtgatac atttcattcg ccgtacgcgg aatacgaatt ttcaccaaat
    22201 ctttataagc gtaataaacc aggccgctgc aatcaaaacc ggtacgcggt gagctgccac
    22261 cccaacgata tggcttacca atttgctgca tcagtttatt cattgccact tttgtggctt
    22321 tttgcacctt cgctttgtgt gcgtcggcaa gggttcctga cgctgcattt ttgactttca
    22381 cacaatgcga tttatagccc ttacgtttgg tacacttttc ggtaaagctg gctgaggccg
    22441 ttttattgac ggtatgtttg ctgcgagttt tggcgttttt agaagcggta atggaagatt
    22501 ttttaacgga agaagcggtt ttgcttttgg tcgtactttt actggcggct tttttcgctg
    22561 ttttctgggt ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat
    22621 gagtggtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca
    22681 gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc
    22741 gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct
    22801 aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg
    22861 agcgcaaaaa aatgcagcag tgaaagctaa aaatggtcat aaatacgcca gtttgccgtg
    22921 catcagggaa gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa
    22981 aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact
    23041 gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc
    23101 agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg
    23161 gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg
    23221 atattctgca gaatccggac attcgtgcgg agctgccgaa atatgctaac tggccgacct
    23281 tcccgcaact gtgggttgac ggcgagctgg taggcggttg tgatatcgtg atcgaaatgt
    23341 atcagcgtgg cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag
    23401 agccggacgc ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt
    23461 cctttctcta tccccaatcc agcccttgtg gcgaacttga aaatcccgct tcacgcagca
    23521 gcgtaaacat cggcgtttgc cagaccggta gatcattcac ttctgttagc gtaaagcgca
    23581 gccgtggttc gcgacgcagt gcggtagtca gcgcgtggaa aacctctggg gcgagtaatt
    23641 cctctttttc ctgccagacc agcatttttt tgccaccttg cgccagatag agtaacaatt
    23701 tgccgccaga aacgaccacc agcgcacccg cccgacgcgt tggtaccagc gttgccggat
    23761 gtgcaggcca gggcagaaga tttccccaca cattagccgg atcgttggcg gagagcgcca
    23821 ctggtgtata gtggcgcgtt tgcgcggctt gtgtcgccag gtcgcgcaag cggtcaatag
    23881 tcagacgttc agcgaattgc gcgccaccca gaccttctac aaaacgacct cgcataattc
    23941 gcccggaatc ttccatactt cgacaaagcg tttgcatcga tggaaacccg ccagggatat
    24001 tttcggctat caccgcctga cgactgatga tgccgtagcg gtcgagcata ttttccgcca
    24061 gcgccagcat cctttcggta tcgtttagtg gctccacctg caataacgac cagcgtccag
    24121 ccagatttgg tgtgttgtaa gatacccgcg gcgagacggg ttgtgcatag acaggacgcc
    24181 cacgacgagc ccggtgactg cggcgagttg aggtgcgtgc gttggaactg ctgcgggtga
    24241 gggcgcgtaa cggtgcccaa atatcgctgg tgatgacgcc ttgccagacc agcgcccata
    24301 acgcctcttg cagtgccgag ggatcaaccg attcgccgat tttgtcgcgt atccgttggc
    24361 tgatttgttg tgcaaaccag gctcctccgt cagccagaac agcgactatc gcttgttgca
    24421 gcgccgaacg attcgcctga tccgcttcgg cgggagtgaa cgattctgca gcatattcct
    24481 gtagatgcag tgccaccagg ccgtcatctt cacccagttt tttttgcccc gaccagataa
    24541 ccgcaccggt tgccagtaat tcatcgagca cttctggcga atagtcgtgt acgcgggcag
    24601 gcaagatctg gctttcccag agtgaggcgg gtaagccgac tccggcaagc tgttcgatca
    24661 cccgcatcac gccatccacc ccctcataaa cgcctggcga tgttgaggca aagagcgccg
    24721 ggctaccatc ggtggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
    24781 tggctgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
    24841 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccaga ccctgttcgc
    24901 gcagttgctg aagctgctct tcgacaatgg caataccgag actaaactca tgagccagtt
    24961 gttcagccgt gaccagagca tgagcccgga gatagcgtag aaagaggtcg cgaagcgggt
    25021 aactgactct atgcaaataa atctctggca gtgactctgg taatcgtacg ccgagggcat
    25081 cgcgcagcct ggcggcatca tccatacagg caagacgctc ctgtccacta atcatcgtcg
    25141 ggaagatgcg ttttactgta agaagatttt ccagatacga ggcaatctct tcactgctgc
    25201 ctgtatgccg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
    25261 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
    25321 cctggcgaat gacctgcggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
    25381 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ttctgccagc ggggcgtcgc
    25441 tctggtacat aaattccgcg acatagccga acaataaact tgtggcgaaa ggcgagggcg
    25501 tagtagtcgt tacatcggat atttgaattt cgcctccgtt caggcgacgc atcaaacgtt
    25561 ccagtgcggg aagatcataa acatcttgca gacattcgcg tagggtttcg agaatggccg
    25621 gaaaatccgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
    25681 aaagcggggt gcgatggccc ggcgtgcggc ccggcattaa taatgcccgc gcggcgcatt
    25741 cgcgaaaacg ggcggcgaaa agtgccgaac tgcctaccgc ctcgcggaca atttgcagca
    25801 acttttctgg ttcaaacaaa aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa
    25861 tgcgcgcaac aatgccgtca tcactggcga ccacagacgc gtcagcgccc caaagcgcat
    25921 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
    25981 gcaaaataat acgccagtcg ccaatttcat cacggcaccg ttcgaggacc agatgacgac
    26041 tccccggaac aatacccgtc gcgttgcgct gctcgtcaat caacccctga atattggcga
    26101 tcgtattttc ttcagccagc cacgggggaa tagtcccgga aaagaacgct ccatccgcca
    26161 gcaaatgaag aaaatcgccg atcatctcgc ctaattcagc cggacgtccg ttaccttcac
    26221 cacgccagaa ggggagccgg gcagaacgac ccggagcagg agtcacaatc acctgatcgc
    26281 gggtgatttg ctggatccgc catgaggttg cgccgagagt gataatatcg ttcacccgcg
    26341 actcatatac catctcctca tccagttcac ccacccgtcg cgaaccggcc tgttcttccc
    26401 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccgccg ctggtaaccg
    26461 ccaacagctg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
    26521 tggggcgaaa agcagaaaaa tcgccagagg gatagcgtcc ggaaagcata tccagcgtgg
    26581 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
    26641 attcgtctac ctgtaatgca tccatcgccg cggcggcaac ggtgtgctgc gcaaggacgt
    26701 cgagtggatt atgcggaggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
    26761 tgactgcgga atcgactaag tcacgcctgg ttcgggggaa aaaaagccct ttggagatac
    26821 ctcctatctg gtgtccggca cgtccaatgc gttgtaaccc actggcaaca gaaagcggcg
    26881 ttgccacctg aatcaccagc tccacagcgc ccatatcaat ccccagttcg agactggaag
    26941 ttgcgaccac gcagcgtaac tcacccgatt tcagcgcctg ctcggtgatt gctcgttgtt
    27001 ctttagagac ggagccgtgg tgtgagcggg caataaaaac gtcgctactt tgtacacggt
    27061 tagaggttgc gccagaggtc gactcgaaat gggccgcatc agcggcgata gacggggaac
    27121 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagccccc
    27181 gcgaattagt aaagacaatg gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt
    27241 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgcca gtgccgctgg
    27301 cgaccgatga gacatcatcc atattggcga ctggtacgac aatccgtatc tgcggatgac
    27361 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaat gctgccacat
    27421 cgctggctga gcgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
    27481 catcgagccg ctccagactt aacgccagat gcgcaccacg tttacttccc gctaccgcgt
    27541 ggacttcatc aataattacc gtttcgacgc cgcgtagcgt ttcgcgcgcg cgggaggtca
    27601 gcatcagata gagtgattcg ggtgtggtga tcagaatatc cggcggatta cgggtgagtt
    27661 tgctgcgttc ctgtgcaggc gtatcgccag tacggatccc cacgcgaaga ttgacttccg
    27721 tttcgccgcg ccgccgccgt tcatcggcaa tacccttcaa cgggatctgc aagttgcgct
    27781 gaacgtcggt gcccagggct tttatcggtg aaatatagag gatgcgtgag gttttacgtt
    27841 tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag tcgatcgagg gcgtagagaa
    27901 atgctgccag cgttttcccg gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg
    27961 ccgccacatg ccaggtttgc gactggacag cggtcggctg tttaaaggcg cgaagaaacc
    28021 agtcgcgggt cgccggtgaa aacacgtccg gcaggggcga tgaagggttt ggattatctg
    28081 ccattaacgc tattccccgt gaatatcatg catgatgcgt ggaatagcct cactatagat
    28141 gcctgaatca ttgcatgtca tcaggcatcg actcgattac acctcttcgg cggcaggtag
    28201 cggccagcct cccagacgtt tccagcggtt gacgatttca caaaacagca ccgcagtgcg
    28261 ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg
    28321 gcaagccttt gacaatacgg tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa
    28381 agtggcgaaa gggtggaacg ggttacgttt cagtgaggcg cgttctgcgg cggccatcat
    28441 aaagctgtga tcgaaattgg cgttgtgcgc caccataatg gcgcggttac agccgctcgc
    28501 tttaatacct ttacgtacaa ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc
    28561 gccgcgatcg ggatcgttcg ggtcaatgcc gttgaaagca agggcttctg gttgcaaatt
    28621 tgcgccgaca aatggttcga cgtggaaatg taatgtggtg tccggcatca gccagccttg
    28681 ttcatccatt ttcagggtga tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa
    28741 tccagctgtt tcaacatcga tcacaacagg ataaaagcca cgaaaacggt cgcacagacc
    28801 ggtaagttga gcgttatcgg acatctgatt ctcttaatac ggataaattg cagcgcgcat
    28861 tatgacaaat attacttgtc gatgcagtaa agatgcgggc gcgatgagtt cgcgcccggc
    28921 aggagattag ttgcccagac cgcgaccggc gtctttctct tcgattaact caattttgta
    28981 accgtccgga tcttccacaa acgcgataac cgtagtaccg ccttttaccg gacccgcttc
    29041 acgggtcacg ttacccccgt tttgacggat tttttcgcac gcttcagcgg cattatctac
    29101 gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat ttatccacgc cccagttgta
    29161 ggtcagttca atcaccgctt cttcggtttc cgggccgtag ccaacaaacg ccagtgagta
    29221 tttgtattcc gggttttcgc tggtacgcag cagtttcatg cccagcactt tggtataaaa
    29281 atcgatggag cgttgcagat cgccaacgcg cagcatggta tgaagaagac gcatcttttt
    29341 atcctcaaaa tggctaacag attcaaaatg ttgttttagt atagcggcgg ctttacgccg
    29401 ctcgcaatgt tggattacaa cgtcgggtaa tcagtgtagc cttccgcacc gccaccatag
    29461 aaactttcag cacgctgtgg gttaagctca gctttgcgct gcaagcgggc gaccagatcc
    29521 gggttcgcaa tccagtcacg accaaatgcc accgcatcaa ttaacccttt gccgatcagc
    29581 gtttcagctt tttctactgt gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg
    29641 cgtacttttt cgcggaacgc atcagtatac ggttcacccc ccgcccaatc tggttctgac
    29701 atatgcagat aagcaatgcc gcgtttaccc agttgttcaa tcagatacag tgcatcggct
    29761 tcttcattcg ggccgttatc cgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca
    29821 atgcgatcgg caccccattc ttcaatcccg gcatcgacca cttccagtac caaacgtgcg
    29881 cgattttcca cgctgccgcc gtactgatcg gtacgatggt ttgaagaagg agaaaggaac
    29941 tgatgcagca aataaccatg agcagagtgg agctctacca gatcaaaacc ggcttcacgc
    30001 gcgttagcaa tagcctgacg gaaatcatgg acgatacttg gaatctcttc cagttcaagc
    30061 gcacgcggca tggatgtttc aacacggatc gcctgaccat tttcatcgcg cagagaagta
    30121 cgtgttcccg cgctaagtgc tgaaggcgct actggtgcct ggccgccagg ttgcaggctg
    30181 gcgtgagaaa tgcgtccggt gtgccacagc tgtacggcca tatgcccatt ttcagcatga
    30241 acgccagcgg taattttttt ccatgcggca atttgttccg gactatggat gccaggtgca
    30301 ccagcatacc cttttgcctg ggcagaaatt tgcgtggctt cgctaataat caaaccggca
    30361 ctggcacgtt ggcgatagta ttccgccatc aacggggtag ggatgtcacc cggttcaata
    30421 ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg ccgcagttat tgcgcccact
    30481 ttcagtgggg aatacagttt ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa
    30541 aatgcctgct aaacggcagg cgtcgcaata atggttttta catgtgccag cgcgttttcc
    30601 agtggctcgg aattgcgcga aattttggcc tgcagattcg cacccagcca tagtgcgtaa
    30661 agcacttgtg cctgttgcag cggttcgcca caaaaggtta aacaatggct atcacggcca
    30721 ttttccagag cctgcgagag cagggcgatc acgccgcgag cgcctttatc catcgcgcta
    30781 cgcatatctt ctgacagatc gcacacttcg gcagagagtt ttactgtcag gcaaccactg
    30841 atggttccat gttggcaaaa ctggttcagt gtttgctggt aataagccag tatgcggtcg
    30901 cggtagttac cttcgccgga ttgcagcaac tcagtcagtc gttggtgata tgtggcgtaa
    30961 tggcgctcaa gcatggcaac gccaaacgct tctttagagc gaaagtagtg atagaaggac
    31021 cctttcggca cttcagcggt ttttagtaat tcgcttagcc ccatcccggt gaatccacgt
    31081 tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc gagtatcatg ttcggtgtgt
    31141 ttgttcatgc gttggagtgt agtagaccag tcggtctagt gcaagcaaag ttgctacttt
    31201 gggcattaac agcttcaata atggtaataa tcttatttga ggagggcgaa gtggcggagc
    31261 aattagagtt ctttcccgtc cagagcccgt gccggggaat ttgccagtct gacgaacgcg
    31321 ggttttgtcg cggctgtttt cgaagccggg atgagcgttt taactggaat aaaatgagcg
    31381 atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg attaatgcgc aaattacgtg
    31441 caaataagcc agcgtcatca gacgaaccgg agcaaccatc actcttttga gcctaatctt
    31501 gcgtatactc ctctcatatc tgataagagg aagcgattat ggttcagcgt attactattg
    31561 cgccgcaagg cccggagttt tcccgttttg tgatgggcta ctggcgattg atggactgga
    31621 acatgtccgc ccgccagctg gtcagtttta ttgaagagca tctggatctc ggcgtgacca
    31681 ccgtggacca tgctgatatt tatggtggct atcagtgcga agcggcgttt ggcgaggcgc
    31741 tgaaactggc acctcacctg cgtgagcgga tggaaatcgt cagtaaatgc ggtatcgcga
    31801 cgaccgcgcg tgaagaaaac gtcattggtc attacatcac tgaccgcgat cacatcatta
    31861 agagcgccga acagtcgcta attaatcttg cgaccgatca tctggatctg ctgttaatcc
    31921 accgcccgga cccgttaatg gatgccgatg aagtggcgga cgcgttcaaa catctgcatc
    31981 agagcggcaa agtgcgtcat tttggcgtat ccaacttcac gcctgcgcaa tttgccctgt
    32041 tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc ccggtacatc
    32101 agccgttact gctggatggc acgctcgacc aactgcaaca actgcgtgtt cgtccgatgg
    32161 cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg
    32221 atgaactggc tgtggtggcg gaggagttaa acgcgggctc gattgaacag gtggtttacg
    32281 cctgggtatt acgtttaccg tcgcagccgc tgccaattat cggctgaggt aaaattgagc
    32341 gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta
    32401 tccgtaaagc ggcactgggg tacgacgtac cgtaagcact tttaggaata gccgccgttc
    32461 aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag gtcctatgaa
    32521 acgttttagt ctggccattc tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga
    32581 aaaagtcgag atgaacctcg tcacgtcgca aggggttggg cagtcaattg gtagcgtcac
    32641 cattactgaa accgataaag gtctggagtt ttcgcccgat ctgaaagcat tacctcccgg
    32701 tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca aagatggcaa
    32761 agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg gtaaacatga
    32821 agggccagaa ggtgccgggc atttaggcga tctgcctgca ctggtcgtca ataatgacgg
    32881 caaagctacc gatgccgtca tcgcgcctcg tctgaaatca ctggatgaaa tcaaagacaa
    32941 agcgctgatg gtccacgttg gcggcgataa tatgtccgat caacctaaac cgctgggcgg
    33001 tggcggtgaa cgctatgcct gtggtgtaat taagtaatta agaggccagc gtaccttgcg
    33061 gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg ccagacaatt gccgccagct
    33121 cacgggcggc aggttgatga tggcgggcaa ggctgtcgca aatccgctgt aattcttcaa
    33181 gtgtggccgc cagtgatttt tgctgaacgc cacgctcact catcactccc cgcaacagtg
    33241 aaatacagtt atcccgtact cgcgataacg gatcggaacg cgattcccag tcgcgtaatt
    33301 gccagacaac atgagaacag ttcagcagca ctatacccca gcgtaataac caacggcgag
    33361 ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg ataagtgagc gattcaaatt
    33421 cgctttcact cagtgttgga tggcggctta gttgatcgac aaaatcccgg cgcagcgcgc
    33481 gaatatggcg gcggcttttg cgagcatccg atcctggacg cagaatggcg aacgctaacc
    33541 acgccaacgc gacgccaacg atttttgcca gattatcgtt aagaaaatca gcaaaattat
    33601 acaccggggg attagtgacg gcgataaaag aacccataaa aacaatcagt tgcccccaca
    33661 atgcggcaaa tttgggcatc tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga
    33721 gaaacagtaa aaattgccac agatcgctaa tctggaccat cagaccaaat ttgaccacaa
    33781 agctgaatag cgaaagtaac accagcgtgc gcatcagcag tgacaacgac ttaaacggtg
    33841 ctgcgacggc ggagtagagt acgcagctaa tcgctgccag cgttaatgca ttggcaccgg
    33901 catcccattg cgaagcaata ctccatgcgc caatcatcat caacgtacaa aatgtacgca
    33961 gtccgctcca catagcttcg gcgttatcgg tatgacgagc caggccggat gtccgtggga
    34021 gtctggcatg atcatctaca tcgctttgca gacgatgtaa ttcctgacta ctttgcagat
    34081 aaaggcggca aaataacgta gtcgctgcca gaaggcgacg tgccgatagt cggcgacgtt
    34141 ggtcgggcgt agcggggcga taatacgtgc gacggtgtaa acatctgttt gcgaactggc
    34201 gagcgccgtc agcaactgtt cgagaatttc tcgcgtggca cctggcggtg agggccagtt
    34261 gagcaacata cgtcgcaggc tggagatgac actggtcata cgtaattgct ggtggagcag
    34321 cgcattaagg cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag cctggatacg
    34381 cagcaaattc atggtcagta tctgcccaat caccccttca tgtgctgcac gaatggcatc
    34441 ggttgtttca ggctgccaga gtaaactggc atgttccagt aatcgggcgt gcatgttttt
    34501 caatgcggtt aaaagggctg tagcatcgga actgctaggc aggatcatca tcatcatgcc
    34561 gccgcacaaa ataccgacaa tcacctcgca aacgcgcgcc tgagcgatat cccacagctg
    34621 gctggcctca gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt agcccgccag
    34681 ttgaaatgca tacgcgacgt tattcgtgaa gtgcgcacag gcccaggtac aaaagccaag
    34741 ccacgccgac atgctcaata gaaaaaacca cggctcattg agcgtatgcc cggcaagaag
    34801 cagtgccgca atggctccca gtaaactgcc agcgatgcgt ccgaggcttt tgctgatgac
    34861 accgccaacg gtgggaaagc taaccactgc agccgaggtc atcgcccaat agggttcatc
    34921 cagattcaaa taataggcaa ccgtcagcgc cagacacatg gcaatggtat tgcgtaacgc
    34981 ataacgccat tgcgccagcg tggccctgaa ccagggcaaa ttgcgcaagg accatgacga
    35041 tgcgttcatt attgacctac ggcaatgcta caggtggtgc ccgacaccag cgtgacatcg
    35101 cctggaactt tatccagcgc aaagcggacg ggaacgcgtt gggcgagacg aacccagggg
    35161 acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctctg atcataaatc
    35221 gcccgaccga tactggaaac gtgaccctgt aacgttttgt tatcactata gagggtaatt
    35281 tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata accaatgacg
    35341 taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc gtaatcgccg
    35401 atgcgggtag tgagattagt cacccatcca ctcaccgggg cgcgaatttc cgtttgcgcc
    35461 agttgccatt gtgtctgttt cagcgtcgcc tgtgcggcat caacactggc ctgcatcgct
    35521 ttaacattga ggttagcggt atcgagttct tcggcagaaa taaaattttg tgagagatga
    35581 cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt cgccagctga
    35641 gcctgtgcat tcagctcggc gatctgaaag ggcgttttat cgatcgtgag cagaagatcc
    35701 cccgcgttaa ccagctggtt gtcttttata ttcagctcaa caatacgtcc cgacacctgt
    35761 ggggtgatag aaacctgttc tgcgcgtatt ttcccgtcgc gtgtccacgg agattgcatg
    35821 taataattcc acagccacca tccggcaaga accgcaacta ccgctacaat gattgttgag
    35881 aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc agagcaaggc
    35941 aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg tcaccggcgt
    36001 agatccagcc acgaagcagg cggtgtacga caagccagat gacgaatcca aacgcgaagg
    36061 ctttgaaaaa cggaggaaag tagacggacg caccgaacac cagatcttgt aagggcaatc
    36121 ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat atattgcgtt
    36181 agattaataa atattcttta actgcgaaaa atttacgcgc aatttctgaa aaatatgtct
    36241 gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc acactattct
    36301 aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga atcgccacta
    36361 ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca tcgcctgaaa
    36421 ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca gttacctcca
    36481 gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc actggtccgt
    36541 actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc cagcgatcgt
    36601 cgggctaaac gtattaaact gacggaaaag gcagagccgc tgatcagcga aatggaagct
    36661 gttattaaca aaacccgcgc ggaaatttta catggcatct ccgcagagga actggaacaa
    36721 ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc caaagggtga
    36781 aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag cgcggagaaa
    36841 cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag aaacgagtgt
    36901 tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt tccagctcga
    36961 caccttgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca cctgcaacag
    37021 cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattt cccaggaaac
    37081 caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg ccctgaatct
    37141 gtaccggacg tacgttaacg atggttccat agctgacatt ctgtacttgt ttcgcttcag
    37201 aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca agagacagac
    37261 ctaccattga aacaaccaat acgcgtttaa tcattgaaaa atctcctgtt caccatgaaa
    37321 cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca tggttacatg
    37381 ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata aacaaaacat
    37441 tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa tcggtcattc
    37501 cgcatggtat tcacgaaaag tttatgtttg agtgtgcgca aacgtcagat tgaaaaagga
    37561 tgaactatga aatcgggccg ctttattggc gttatgtcag gcaccagcct tgatggtgtt
    37621 gatgttgtgt tggcgacaat tgatgaacac cgggtcgcac agctggcaag tttgagctgg
    37681 ccgatcccgg tgtctctgaa acaggctgta ctggatattt gccagggcca gcagcttaca
    37741 ctttcgcagt ttggacagct tgatactcaa ctcgggcgac tttttgctga tgcggtcaag
    37801 gccttgctta aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt
    37861 caaaccgtct ggcatgaacc gacgggcgtg gcaccacaca ctttacagat tggcgataac
    37921 aatcaaattg tggcacgcac cggaattacg gttgtcggtg attttcgccg tcgcgatatt
    37981 gccttgggag gacaaggcgc accgctggta cctgcgttcc atcatgcctt gctggctcac
    38041 ccaaccgagc gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc actgctcatt
    38101 cctgggcagc cggttggcgg ctacgatacc ggtcctggta acatgctgat ggatgcctgg
    38161 atctggcgtc aggccggtaa accttacgat aaagatgccg agtgggcacg ggcgggtaaa
    38221 gttattctcc cactgctgca aaatatgctc agcgacccat atttctcgca acctgcaccg
    38281 aaaagcaccg gacgcgaata ctttaactac ggctggctgg agcgccattt acgccatttt
    38341 ccgggtgttg atccccgtga tgttcaggcc acactggcag aactcaccgc cgtgaccatt
    38401 tctgaacaag ttttgttgag cggtggctgc gaacgattga tggtatgtgg tggaggtagt
    38461 cgtaatccgc tactcatggc gcgtctggcg gcattactgc caggcacaga agtcaccacc
    38521 accgatgccg ttggcattag tggcgatgac atggaagcat tggctttcgc ttggcttgcc
    38581 tggcggacgc tggcgggatt accaggaaat ctgccttccg tcactggcgc aagccaggag
    38641 acggttctgg gggctatttt ccccgccaac tcgtgatatc agaattaact gaattttttc
    38701 ttatcgtgtc tgccgttaca cttggaagat taaggaggat atttgccctc cggaccagga
    38761 aagtcttcgg gatatgacta tgaaaaaact gttaattatc attttgccgg tccttctctc
    38821 cggctgtagc gcctttaatc aactggttga gcgaatgcaa accgatacgc tggaatacca
    38881 gtgtgatgaa aaaccgttga cggtcaaact gaataatccg cgccaggaag tcagttttgt
    38941 ttacgataat caactactgc atctcaaaca gggcctttca gcctctggcg cgcgttactc
    39001 cgacgggatc tatgttttct ggtcgaaagg tgaggaagcg actgtctata aacgcgaccg
    39061 catcgtcttg aataactgtc agttacaaaa tccacagcgt tgagattttt ccaggggcgg
    39121 cgcacaatag cgtcacccac agacaatccg taaagaaaac catgtctgat aacgacgaat
    39181 tgcagcaaat cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc cgccgcgatc
    39241 ttcccgccga tccattaacc ctttttgaac gttggctctc tcaggcttgt gaagccaaac
    39301 tggcggaccc taccgcgatg gtggtcgcta ccgtggatga acatggtcag ccttatcagc
    39361 gcatcgtttt actcaaacat tacgacgaaa aaggcatggt gttttacacc aacctcggta
    39421 gccgtaaagc acatcaaatt gaaaataatc cgcgcgttag cctgctgttc ccgtggcata
    39481 cccttgaacg ccaggtgatg gtgatcggta aagcagaacg actttcgact ctcgaagtga
    39541 tgaaatattt tcatagccgc ccgcgtgata gccagattgg ttcatgggtt tcgaagcagt
    39601 ccagtcgcat ttctgcccgc ggtatccttg aaagtaaatt cctggagctg aagcagaagt
    39661 ttcaacaagg cgaagtgcca ttgccgagct tttggggcgg ttttcgcgtc agccttgaac
    39721 agattgagtt ctggcagggc ggtgagcatc gcctgcatga ccgctttttg taccagcgtg
    39781 aaaatgatgc gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt
    39841 aatcgctggt actcctgatt ctggcacttt attctatgtc tctttcgcat ctggcgaaaa
    39901 gtcgtgtacc ggcaaaggtg cagtcgttat atacatggag attttgatgg caagcagtaa
    39961 cttgattaaa caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt
    40021 agcagagcga ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc ctaccgctga
    40081 cagcttgcat ttggggcatc ttgttccatt gttatgcctg aaacgcttcc agcaggcggg
    40141 ccacaagccg gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa
    40201 agctgccgag cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg
    40261 caagcaggtt gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa
    40321 caactatgac tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca
    40381 cttctccgtt aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga
    40441 tcaggggatt tcgttcactg agttttccta caacctgttg cagggttatg acttcgcctg
    40501 tctgaacaaa cagtacggtg tggtgctgca aattggtggt tctgatcagt ggggtaacat
    40561 cacttctggt atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt
    40621 tccgctgatc accaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg
    40681 gttggatccg aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgga
    40741 tgccgacgtt taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc
    40801 cctggaagaa gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga
    40861 gcaggtgact cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga
    40921 atgcctgttc agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca
    40981 ggacggcgta ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cgctggtcga
    41041 ttctgaactg caaccttccc gtggtcaggc gcgtaaaact atcgcctcca atgccatcac
    41101 cattaacggt gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt
    41161 tggtcgtttt accttactgc gtcgcggcaa aaagaattac tgtctgattt gctggaaata
    41221 atgcattaag tggaaagggg gagtgagaaa tcactccccc tggtttttat acagggaaca
    41281 tgatgaaaaa tattctcgct atccagtctc acgttgttta tggtcatgcg ggtaacagtg
    41341 cggcagagtt tccgatgcgc cgcctgggcg cgaacgtctg gccgctgaac accgttcaat
    41401 tttctaatca cacccaatac ggcaaatgga ctggctgcgt gatgccgccc agccatttaa
    41461 ccgaaattgt gcaaggcatt gccgccattg ataaattaca cacctgtgat gccgtattaa
    41521 gtggctatct gggatcggcg gagcagggtg aacatatcct cggtatcgtc cgtcaggtga
    41581 aagcagcgaa tccgcaggcg aaatattttt gcgatccggt aatgggtcat ccagaaaaag
    41641 gctgtatcgt tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg
    41701 atatcattgc gccgaatctt gttgagctgg aaatactctg tgagcatccg gtaaataacg
    41761 tcgaagaagc ggttctggca gcgcgcgaac tcattgcgca agggccacaa attgtattgg
    41821 ttaaacacct ggcgcgagct ggctacagcc gtgaccgttt tgaaatgttg ctggtcaccg
    41881 ccgatgaagc ctggcatatc agccgtccac tggtggattt tggtatgcgc cagccggtag
    41941 gtgttggtga tgtgacgagc ggtttactgc tggtgaaact gcttcagggg gcaacgctgc
    42001 aggaggccct ggaacatgtg accgctgcag tctacgaaat catggtgacc accaaagcaa
    42061 tgcaggaata tgagttgcaa gtggttgctg ctcaggatcg tattgccaaa ccagaacatt
    42121 acttcagcgc aacaaagctc tgaaatatct catttcggcc cacaacgaac tgtgggccga
    42181 cggctattac tttaagcctt ccgctgacag cgcgtcttgt acttccggac gttcagccat
    42241 acgttgcata aatgccgcaa tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc
    42301 ccagcgcagt accgtaaaca gataggcatc agcaattgta aatctttgcc cgcagatcca
    42361 gtgctcatcc ttcagtgcct cgttcacata ttgcagtttc ttctccagct gcgcgcgaac
    42421 tgtcggtttg tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag
    42481 ctcggtggcg atgtaattca gccattcgat ggttttatag cgggaaatac tgtttaccgg
    42541 tgccagcaac tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg ctacaccttc
    42601 cgtcagcaaa gtaccgtcat ccagcagcaa tgcaggtacc tgtcccttag ggttaacggc
    42661 aaagtaatcg tcaccgtttt cgagacgttt tttcattaaa tccacactga cgagcgtaaa
    42721 atcctttccg ctctcacgca gggtgatatg ggaagcgaga gagcaggcgc ccggtttgta
    42781 gaacaatttc atcggtaact ccttttgtct gtggtttaaa gtatggtagt gcgttgctga
    42841 acaaaaaaaa agccgctaac gaaaagttag cggcttgtaa aagagtttcc ctgaatttta
    42901 cgctacggct gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatgc ggtgcagttt
    42961 cggcgcggtc agcagcatca gtactgcaat aacggcagta gcgacaccaa tctgcaagaa
    43021 tacgcgacca tagacttcca gtgacatcag cggatcggta acgttatccg gcacagccat
    43081 catacccgca acataaccac caatcaggtt tgcaccggca gtggtcagga accagctacc
    43141 cataatgaag cccatcagac gctgcggaac gagttgtgca accattgcca gacccagacc
    43201 agagatcatc agttccccga tactctgcag gccatagctc gcgaccagcc agcttacaga
    43261 cacgatacct gcgtcagacg cgaatttcgc acccagcggc agaatcagga acgcaccaga
    43321 acacatcacc ataccgattg caaacttggt tggcatcggc agggtatcgc ccatcttgtt
    43381 atagatagcg gccagaatcg ggctaccaat gatgatccag aacgggttca gtgcctgata
    43441 ctgctcaggt tctacggcca ggcccagaat ggtatgttca acgttacgaa tcgcaaagaa
    43501 gttcagtgac gttggcatct ggctgtacag cacgaagaag ataatggctt cgagcatcag
    43561 gatgaaggca acgatcattt tacgacgcgc agcacctttc atcgcgaagg cttctttacc
    43621 gaagataacc acgataccga aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt
    43681 gtgcagcagc caggtggcga tagcgatcag tgccacaaca ccaataatgg tcagcagcag
    43741 gttacggtag ttgataggct cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg
    43801 gcagaaggcg aagttaacga tagtgatcag caggcctaca acgctcaacg caaacgcaac
    43861 actccagccg tatttcgcgg ccagccacgg ggtggcgatc atagagaaga aagagccgat
    43921 gttgacggac atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt ttttctcata
    43981 gcatgtagaa agcagagaag acgggttagc tttaaacagg ccgttaccga ccgcaatagc
    44041 cgccataccc atataaacga taccggcgtc gtgaccagac caggcaacca gagcataacc
    44101 aatcgccagc acaatagcgc cgagcataat tacgcgttta gtacccagta ccttgtcacc
    44161 taaccagccg ccgatagcga ccagaccata aaccagggca ctaaaggaag agaaaagggt
    44221 gattgaatcc gcttcagaca tacccagttg tttaaccagg taaacagcca taattccttg
    44281 taggccgtaa taaccaaaac gttcccataa ctcaatcgag aagatgagat agaacgcctt
    44341 cggttgtttg aaagcgttca aactgacgct ttcagttggt ttttggtttg cagtggacac
    44401 ttttacctct ttttttacat cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa
    44461 aacatacgcg caattgttat agtgggaggg gaaacggcag gtaatgttca ctatcttgca
    44521 ctttcagaca agtcctttgt aataatctgt tacatataac tgaggcgggg aattcaacca
    44581 ttccgggaaa tgttatccag cgttaaattt ttcaaaaaac gtaacggcag attttttcac
    44641 tgcattgaga gggggatgat ggcttgatgg cgatatgttc tgctatttag cgtttttcct
    44701 agtgagatag tccagtttct taaaaatagc cagtgtaatg ttttgtaggt caatatttgt
    44761 tgcgtgtgta aatttaaatg attttaacct gattcttgca atgtttttac attgccacag
    44821 catcgtgatc ttgatcacta atgaatagaa aatttcgcta ttaaaaaaac aattaacctg
    44881 catttttggt tatccgcaaa cggattacct gatgataacg ctaagcaatg gcatcattga
    44941 cgttaaaaag atgttttaaa agaggataaa gaaaggttat caatggggta atcggtgtta
    45001 ccccttttct tcagatgtca actttctctt tgtattcaca aagatcttca ataatacaag
    45061 agccacagcg gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc aaccaatggt
    45121 ggcagtcgac tttaaactct gccggaacca ctttcagtag cttttcttct acctgttcga
    45181 cgtttttccc cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag
    45241 caatagtcgg ccagccgaat gcagtgttta atacgacgtt ggctgtttta cgacctacgc
    45301 cgggcagggc ttcaagcgca gcacgatctt ccggaacctc gctattatgc tgctccagta
    45361 agatacggca ggttttgatg atattttctg ctttgctgtt ataaagccca atcgttttga
    45421 tataggtttt caccccttca acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg
    45481 ggtagagttt cgccgtcgcc ttattaacac tgacatcggt tgcctgagcg gaaagcagta
    45541 cggcaatcag caattcaaaa ggcgaactga aattaagctc agtggtggga tgaggattgt
    45601 tctcacgcag gcgagtgagg atctccaggc gttttgcttt gttcatcaga cattccctgt
    45661 ttcaccgttt ggcaatgcac gttcggcagc tgcttcagta cggcgctttt tcattctttc
    45721 atcaatcagg tattttcctg ccagcatcag tcccaggcca ataaatgcac ctggtggcag
    45781 catcgccagc aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata cttttgccca
    45841 gctacctaac agcgcatctg caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag
    45901 tgaacccagc acgaacatgg cgcaggttgc gcccatacca attgaaaagc cgtccagtgc
    45961 cgaaagcgcc ggaccttttt tggcagcgaa ggcttcagcg cggcccacaa cgatacagtt
    46021 agtgacaatc agcggaataa aaatccctaa tgattgatac aggccaaagg cgtaggcgtt
    46081 gatcagcatc tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg
    46141 gatctcggct ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac
    46201 cagcgtagtc gcaagtccta aacccagagc gttagtggca gtagacgtga cagccaacag
    46261 aggacaaagg ccgagcaact ggaccagcgc agagttgttt ttccacaacc cctgaacaat
    46321 aacgtcttta atttcgctca cgctttattc tccacaggca ggaagttgag aaagttgttc
    46381 cggtaacgtc tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg cgcggggagt
    46441 aatcgtcgcg ccggtgaact ggtcgaaatt accaccatct ttcttcaccg cccagtgcgc
    46501 atcatccgca ccactgattt ttttacccgc aaaatgggtg atccagtcag aaaggcgcag
    46561 ttcgatttta tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac
    46621 cgtgccgtta aaatcggctc ccaccagcag ctgaatcgca ccggaatagc catctggcgc
    46681 ggttgcttcc agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg
    46741 ctcaccttta cctaactctg gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt
    46801 atagcgttcg gctggcagca cctgatcaaa taaagccttt tgttgcagac tggcctgttc
    46861 agcaatcgtc gttttggtca tctggttgat ggccgcagtt aaccctgttg aacccgctgc
    46921 aaatagcgcc aacgtaatgc cgtgttttcg gatagttttc agcatggttt accctttgcg
    46981 atggccgtag acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag
    47041 caggacggca aaagccacgc cgtcaggata gccgccgaaa ctgcggatca accagactaa
    47101 taagcccgca agcgcgccga aaataagacg accacgattg gtcgtagaag cggtaaccgg
    47161 gtcagtcaaa ataaagaatg cgccgagcat ggtcgctcca gacagcagat gaatttgcgg
    47221 tgctgccagt gtttctggtg agaacaacca gcccaacgtt gcgcataacg ccagcgttac
    47281 taagaagctg aggggaatat gccagcgaat cgctttctgc catagcaacc atacgccgcc
    47341 agccagccag gcgagattta cccattgcca accagcgccc gccagaatac cgctgtagat
    47401 cggatattgc ataatctgtt caaccgaatg accggcacgg acagaggttt taaatgtatc
    47461 cagcggtgtc gcctgactaa tgccatcaat acctaagcgt agtgtgttca tatcaccacc
    47521 actggcggta tgcccgctga aaataacctg tatggcgtcg ataaaaccag ggatgttgac
    47581 cgcaatttca tgtggcggta accagctggt catctgcacc gggaaggaga tcagtaagac
    47641 cacataacca atcattgccg gattaaacgg gttttgcccc agaccgccat acaactgttt
    47701 agcgatgata accgcaaaca ccgtacccag cacgaccatc caccatggcg cgaggggggg
    47761 aatacttacc gccagcaata agcctgtcag caatgctgag ttatctttca acgtggcggc
    47821 taccgactgc ttgcgtagtt tgagtacgag agcttcggct aacagagcac taaccgatgc
    47881 caacaggatc tgaacgagag taccccaacc aaaaaaccac agttgcgctg cgattcctgg
    47941 cacggctgcg agcaacacca acagcataat gcgcgatgtc tggcgctggt tatgggtata
    48001 aggggagcta gctattctga ataccattta gtcctcgttt acaacctttt gctggacggc
    48061 ttttttggcc tgaacgcggg caatagccgc ggcaactgcc gctttgcgcg gatcaacctg
    48121 ttcttctggt tctgcattag cctgttgctg ttccagcttg cgtgctttgg cgcgtgcaat
    48181 agccgctgcg acggcagctt tgcgcggatc aacctgttct tctggctccg cattagcctg
    48241 ttgctgttcc agtttgcgcg ctttggcacg ggtaatagcg gcttcgacgg cggctttgcg
    48301 cggatcgacc ggttcttctg gttccgcatt agcctgttgc tgttccagct tgcgcgcttt
    48361 ggcgcgggcg atagcggctt cgacggcggc tttgcgcgga tcgacctgtt gttctggttc
    48421 cgcattagcc tgttgctgtt ccagcttgcg tgctttggca cgggcaatag cggcttcgac
    48481 ggcagctttg cgcggatcga cctgttgttc tggttccgca tttgcctgtt gctgttccag
    48541 cttgcgcgct ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagtaac
    48601 ggttgctgcg tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg
    48661 ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat
    48721 aggctgtgta gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc
    48781 tttatctttg gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt
    48841 ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt
    48901 ttcttcctga cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg
    48961 aatattgctc gggcaaaccc acgcgcaagc cccacattca atgcagtcgg caatgttatg
    49021 cgtggtagct ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag
    49081 atccgcaggg caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg
    49141 ttcgccaagc tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac
    49201 atccagccat ggcaaggtaa agcccattag cgggccgccc ataatcacca tttgatcggc
    49261 agaggggcag aatccggcgt cattcaataa atgacgcact ggcgtcccca gccgtgccca
    49321 gacgttgccc gggcgagcga ttgcttcgcc agtcagggtt acgacacgct cggtaatcgg
    49381 ctcgccatca ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac
    49441 gccgatatcg gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa
    49501 ttgtttagca ccgccagaag gatatttggt tggaatcacc cgcatagaaa tatcgtgaga
    49561 gtccgccagc accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat
    49621 aagaatttcg cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc
    49681 cgcgcaatcc tgcatcaaac ggtcatcggc ggtaatgtat ggctcgcact cagcagcgtt
    49741 gataatcaac gtctcaatct tatctccgcc gccctgcaat ttaacgcctg tcgggaatcc
    49801 tgcaccaccc agcccggcaa caccaaactg atgaatgcgc tcgattaact cttcgcgacg
    49861 gcgagagcga taatcggccc agccgtctcg tgggatccag cagtcttcac catcggcatc
    49921 aataatcacg cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc
    49981 cgtaacggta cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag
    50041 cggctggccg cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat
    50101 atgctgtttc agtggaatga caaaacgctg cgccagtggt acctggcgca ggggtgtacc
    50161 gttggactgg gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt
    50221 ttttctgaat gcagagaata acttaagcat ggtgttccac gggaatgata cgcacgggaa
    50281 tggtgttcag atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc
    50341 agtgcgtcgg gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg
    50401 tatgcatggc acgggtagcg ccaacgatgg cgtctaccgg acacgcctga atacatttag
    50461 tgcagccaat acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttagctctt
    50521 gcgcttcgcc atccagcggc tgcggctcga cattaagcaa ctcggcaatt tttagcatca
    50581 cagcttcgcc accaggggcg caacggttga ttttttcacc gttacagctg atggcttccg
    50641 cgtaggggcg acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt
    50701 caattttctc aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa
    50761 tggcgccaaa cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca
    50821 ttacaacttc accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat
    50881 taacgcaatg gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg
    50941 gatggctgcg aagagaacca tcaccagcga aaaaccgaca gcggcggaaa aaccgtacag
    51001 cgccgactgc aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcacggc
    51061 acagttggtg gtgataagcg gcaaaaaaat ccccagcaaa cggtaaagca ccgggctggt
    51121 tttgcgcacc accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag
    51181 ggtgcgcagg taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca
    51241 aatagacgcc agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag
    51301 ctttttggaa acccccataa acggacagag accgagaaac ttgaccagta caaagttatt
    51361 gaccagtaca gttccgacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa
    51421 aaagccgcct attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt
    51481 acggattcac aaacgtcgct ttcacccgcg acgagcgttt gaaatagggg acgatcaatg
    51541 cggttgccaa caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa
    51601 aagcgaacgc ttttaccgcc agcaatacgg aaataagcag ccagataata tagtgtttag
    51661 gaacacaacg ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg
    51721 cgatagcggt aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtgcgccga
    51781 gcgtttgaaa tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg
    51841 tacttaacag agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt
    51901 gtggcgttgt ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt
    51961 taggggcgtt attataaggc ttttataacc gatcgctact ttctttattg aacatagtgc
    52021 cagacggact tcggtacctg gccgaggtca aacaaacgcc cgccagaaac cagctctgcg
    52081 cgacggtgat cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa
    52141 ttaagatggt cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat
    52201 aagtagtcct gaacagtcat aaaaggtcca taaaataatt aataccaaag gggtaggtat
    52261 tattagtgat gataaaattt caccaaaagt gaacgcttgt gttattacaa taataagaat
    52321 gcgctgatgg caaatattca gggatgaaaa actgctatga aaagagtaac gcgctttact
    52381 atccctgaca atcaccaaca acaccgaaca agataataaa ttcctggttt aatatccgac
    52441 aagtgaaaac atgcaccctg acgggcagca tgtcgctcta caagcgcagg gcttacttgt
    52501 gttgtaccgt agtactctgt tcaggtggct gatagttgtc aatgtgactc gccacgccaa
    52561 gaagaatgac tgaaacgaca agaacgatcc aacctgttaa ttcaataaga cgattcatta
    52621 caactcacat ctctgttgat tgatccatta acttcagggg gtaaatgtta cttagcaata
    52681 atagctccag caagattttt actgaggttt ttgcgatatt tagcttttgt cgttggaaaa
    52741 atcgctattt ttttgactta agttaaacaa catcccttat tgctggcagg ttattaaact
    52801 gttgagcgag ggtaaaggat agtgtcaaat agccatcata cttcaatgag aggcaatgac
    52861 atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa aaccttccat
    52921 gcgcccctga ttgcgggcac acccgggctg gaactggcgg taatctccag cagtgatgaa
    52981 acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa gcatctgttt
    53041 aacgatccca acatagacct gattgtcatt cctacaccca acgataccca tttcccgtta
    53101 gccaaagcag cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt taccgtgaca
    53161 ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt tctgtctgta
    53221 ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct cgcggaaggc
    53281 gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc gcaggtgcgc
    53341 gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt agcaccacat
    53401 cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt agatttggca
    53461 cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc ctatccacag
    53521 cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg gtatatcgtg
    53581 catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga acgtctgaaa
    53641 aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg cgtacttacc
    53701 cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtgcctgg gaattatccg
    53761 gcttactatg cggcgattcg tgatgcgtta aatggcgatg gtgaaaatcc ggttccggca
    53821 agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc caaacatcgc
    53881 gcgactttgt gccttgcatg aaaaatggat ggtgcaattt gcaccatcca tttttgttac
    53941 ttcgctgcga ctttttctcg cagtgcgcgt ttttcctctg cgttgaggaa agccatttcc
    54001 agaccattaa tctgtgcctg gcggatttgc tcgcgggata acccagcagc tggcgcggca
    54061 acggtatatt cgtgaatgat atccactccc tgtacgccgg gatcgtcagt gttaatgctg
    54121 gcacgaatgc catgctcaag gaacgttttc agcggatgtg cagccagctc tgccacggtg
    54181 ctggtctgaa tattggaggt cagacaggat tcaataccaa tttgttgctc ggcgagaaaa
    54241 tccatcagcg cccgatcttc aatggctttt acgccatgtc caatgcgctc tgcccccagt
    54301 tcacgaatcg cctgccagat gctttccggc ccggcagctt cgcctgcatg gacggtaata
    54361 tgccagcccg catcacgcgc gcggttgaag tgagaaagaa acagacttcc cgggaaacca
    54421 agttcatcac cggctaaatc aagtgcggta atctggtcac ggtgggctaa aaaggcctcc
    54481 agctcttgct gacaggcggc ttcgccgaag gttcggctca taatgccgat aagcttcgcc
    54541 tgcacaccaa aggtgcggca accttcacgt acgccatcga tcaccgcttc aacaacacct
    54601 gcaacaggca gctgatgtgc cattgccatg tagcctggtg aaaaacgcag ctcgacatag
    54661 tgcaggccgt tacgggctgc atcttcaatg ttttcaaatg ccacgcggcg acaagcatca
    54721 agagaggcga gaactttaac gccccagtca agtttggtca gaaagctcac cagatcgggt
    54781 tcgttggcaa tgacctgaac gtggggaatc agtgtttcca gggattgtgc aggaagcgag
    54841 atattatact ggcggccaag ttcaagaatg gtctggggac gaatgttgcc atcaaggtgg
    54901 cgatggatat cagttaatgg cagggtggta tcaatcatgg tcgcactctt tttctggata
    54961 taaaagtgcg caccattata aaaacaaagc agggtaaaaa tccacgattg cgcaacgatt
    55021 ttttacgttg cgcaattggg gttagcgaac agcgcggatg gcgttaatta atccagccac
    55081 acctttttcc agtttcgaac gtgggcagcc ggcattgagt cggacaaaac cacgaccttc
    55141 ttcaccgtag gtataccccg gcatgatcgc gactttttct tgttcgataa gtgctttttg
    55201 caacgcgttg tcgtcaatat tcaacggacg taaatcaagc caggccagat aagtggattg
    55261 cgggatctgc cagttgagtt caggaaacgc ggcgttcatt ttatctgcga tatacgtcag
    55321 gttatctttc agatagacgc gtaaggcatc cagccacggt gcaccttgct gataggcggc
    55381 gatatgggca gttaacgcca gtaccgaagg ggaagaaagc ccatcacgtc ctttcagtgc
    55441 cgataaatag gcatcacggc tgctgctatt ttctataatc ccgtaagcac cggtcagcgc
    55501 gggaatattg aaacttttcg atcccgacgt cagcaacgcc cagtctcccc gcgccacatt
    55561 actccaggga atatgcggct gctcgcccca aaccatatcc atatggattt catcggaaat
    55621 aacccgcaca ccatgacgct cgcacaggtc agccatgatc tccagctcat cgcacgtcca
    55681 cacttttccg gtaggattct gtgggctaca caggagcata attttacatt ctggtttcgc
    55741 caacacggct tccagcttgc ccatatcgca aaaccagccg tcagcctgct tctctaaagc
    55801 aacgggcatt actgtgcgct gattaccttc aatggccttg taaaatgcgt cataggcggg
    55861 tgtgtggatc accacgcctt cacctgtttc agaccactga cgaatcagtt ctgagaccat
    55921 atagataaca gaagggccat acaccaccga ctgaggatcg atggcggtgt aatgctgggt
    55981 ggaaaaccag tgggcaatag ccgcgagaaa ctcatcgttc ttccagcggc tgtagccaaa
    56041 cacgccgtgc atcaggcgct gattcagcgc ctcgataatg cagggggcag tggcaaaatc
    56101 catgtctgaa atcgtgaaag gtaacaggtc agcagtgccg aaacggtcag cgacataatc
    56161 ccactgcgta caccatgtgc catgacgatc cacgaccttt gaaaaatcga acatatctta
    56221 tccttatgcc tggacagtat gcatcagacc ggccatttca tcttttactg actgaacttg
    56281 tggcccgata acaacctgca ggttatgttg attaagttgt actacgccaa ttgcccgatt
    56341 gtccttcagt gcctgcacat taacaagcga catatctttc acagacaaac gcagacgggt
    56401 aatgcagtta tcgaggctaa caatattgtc ggcaccgcct aaggcttcga ggattgccgg
    56461 aacgttgtaa cctgatttac ccggcgcacc ggcaacggct ttttcgattg aactggccac
    56521 ttcgctatcg cgccccgggg ttttcagatt gaagcgggtg atagcgaaac ggaagatgac
    56581 gtagtaaacg acaaaccaga ttgccaccac cactggcacc atgtaccact tggttgacag
    56641 accatgcaaa ataccgaaca ccacgaagtc gatgatattg ccgtcggtat taccgatggt
    56701 gacgccgagc acagacatga cggtgaagcc gaggccggtt aacagcgcgt ggatgacata
    56761 cagaactggc gctacgaaca ggaacaggaa ttccagcggt tcggtcgtgc cgccaacgac
    56821 acaggcgatc aggccagaaa tcagcagccc tttaatttta tggcgatttt ccgggcgcgc
    56881 gcagtgatac atcgctaacg ctgcacctgg cagaccgccg agaaacgcag gcattttgcc
    56941 ttgcgaaagg aaacgcgtgg cgctttcaga aaaaccgtga gtggtcgggc aactcaattg
    57001 cgcctggaag atggtcaatg cgccgctgac ggtttgaccg cagacttcct gcgtgccacc
    57061 tgcgtcggta aagcgaatta atgccaccag aatgtgatgc agaccaaacg gcaacagcag
    57121 acgttcaccg gtaccaaaca gcatcggtcc gaaatcaccc gcgctattga tcatatggcc
    57181 taagccgcta atacccatgg cgaaaatcgg ccagactaat ggaatcacca ggccgacaag
    57241 gcccatcacc agcgaggaga tgattggtac gaagcgcgta ccgccgaaga atgccagcgc
    57301 atccggcagg cggatattat ggaatcgctc atgcagcatc cagacgataa taccggcgat
    57361 caccgcaccg aggatcccgg tgtcgatcga ctggatccca agaatgctct ggatgttatt
    57421 ggctttcaga acggcggcat ccgtggttgg cagaatgcct ttattggtca accagaagtt
    57481 taccgcgagg ttcattaccg cataaccgac gaagccagcg aatgccgcta cgcctttgtt
    57541 ttctcgtgcc agacccagcg ggatggcgat acagaacatg actggcagga aactaaaagc
    57601 aaacgagcca atcttactca tccaggtaaa gatagcctgc aacacggggt tgcccaggac
    57661 cgggagcagg gtgatgacat cgtggctact aagggaacta ccaatgccga gcataatgcc
    57721 gcagaacgac aataatgcca cgggtaacat gaaggttttg cctaactgct ggaagaactc
    57781 ccacagcgtg actttcggtg ctgttttcgc cgtcataaaa cgactcctcg tagagaacaa
    57841 tctggattca gtaaattgcg cgagaagata aaacgtttta tcaaatttta gtgaggcata
    57901 aatcacatta cgcaacgata atagcgggta taagataaat aaaaggtaaa acgttttatc
    57961 tgtcacaaaa tcagggagta ggtcatctgc atggctaccg ccaaaaaaat aaccattcat
    58021 gatgttgcgc tggctgcggg cgtgtcggta agtaccgttt cgctggtgct tagtggcaaa
    58081 gggcgaatct ctaccgccac aggagaacgc gttaacgccg ccattgaaga gctgggattt
    58141 gtgcgcaatc gccaggcgtc ggcgctgcgc ggtgggcaaa gcggcgtcat tggtttgatc
    58201 gtccgtgatt tatctgcgcc gttttacgcc gaattgacgg ccggattgac ggaagctctg
    58261 gaagcgcagg gacggatggt ttttttgctt cacggcggta aagacggcga gcagctggca
    58321 cagcggtttt cactgttact gaatcagggt gtcgatggtg tggtaattgc cggggctgca
    58381 ggaagcagcg atgacctgcg acggatggca gaagaaaaag ctatcccggt gattttcgct
    58441 tcccgtgcca gttatcttga tgatgttgat acggttcgcc cggacaacat gcaggctgca
    58501 cagttgttga cggagcatct cattcgcaat gggcatcagc ggatcgcctg gctgggaggg
    58561 caaagttcct cattaacccg tgcagaacgg gtggggggct attgtgcaac tctactaaaa
    58621 tttggcctgc cgtttcacag cgattgggtg ttggagtgca cttccagcca gaagcaagcc
    58681 gcggaagcta tcacggcgct tttacgtcat aacccgacca tcagtgccgt ggtttgctat
    58741 aacgaaacta ttgcgatggg ggcatggttt ggtttgctga aagcagggcg gcaaagcggg
    58801 gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc tggcggcatt taccgatgcg
    58861 acaccaacca cacttgatga tatacccgtt acctgggcca gcacgcctgc gcgggaactt
    58921 ggtaccacac ttgcggatcg catgatgcaa aaaatcaccc atgaagagac gcattcacgc
    58981 aatcttatta ttcccgcccg gctcattgcg gcgaaataat cctctcttta tctgctatac
    59041 ctggtagtgt cctttcctca agtttaatga taaaaaatgt ggcacacagg cggtgccttt
    59101 tatttttact gaagttcaca ggaggtttat gtgtttaatt ctgacaacct gagactcgac
    59161 ggaaaatgcg ccatcatcac aggtgcgggt gcaggtattg gtaaagaaat cgccattaca
    59221 ttcgcgacag ctggcgcatc tgtggtggtc agtgatatta acgccgacgc agctaaccat
    59281 gttgtagacg aaattcaaca actgggtggt caggcatttg cctgccgttg tgatattact
    59341 tccgaacagg aactctctgc actggcagac tttgctatca gtaagctggg taaagttgat
    59401 attctggtta acaacgccgg tggcggtgga cctaaaccgt ttgatatgcc aatggcggat
    59461 tttcgccgtg cttatgaact gaatgtgttt tcttttttcc atctgtcaca acttgttgcg
    59521 ccagaaatgg aaaaaaatgg cggtggcgtt attctgacca tcacttctat ggcggcagaa
    59581 aataaaaata taaacatgac ttcctatgca tcatctaaag ctgcggccag tcatctggtc
    59641 agaaatatgg catttgacct gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg
    59701 gcaatattaa ccgatgccct gaaatccgtt attacaccag aaattgaaca aaaaatgtta
    59761 cagcacacgc cgatcagacg tctgggccaa ccgcaagata ttgctaacgc agcgctgttc
    59821 ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg
    59881 gtacaggagc tcaattaata cactaacgaa ccggtaaaca gccgtatgtg ttgtttaccg
    59941 ggatagcgtg tgcaattcat caacgtctct gctaaacaac tggcagccaa atcacggcca
    60001 ttggttaacc aatagctgga tgaaaagcat acgaatagag tgtgccttcg cactattcaa
    60061 cagcaatgat aggcgctcac ctgacaacgc ggtaaactag ttattcacgc taactataat
    60121 ggtttaatga tggataacat gcagactgaa gcacaaccga cacggacccg gatcctcaat
    60181 gctgccagag agattttttc agaaaatgga tttcacagtg cctcgatgaa agccatctgt
    60241 aaatcctgcg ccattagtcc cgggacgctc tatcaccatt tcatctccaa agaagccttg
    60301 attcaggcca ttatcttaca ggaccaggag agggcgctgg cccgtttccg ggaaccgatt
    60361 gaagggattc attttgttga ctatatggtc gagtctattg tctctctcac ccatgaagcc
    60421 tttgggcaac gggcgctggt ggtcgaaatt atggcggaag ggatgcgtaa cccacaggtc
    60481 gccgccatgc ttaaaaataa gcatatgacg atcactgaat ttgttgctca gcggatgcgt
    60541 gatgcccagc aaaaaggcga gataagccca gacatcaaca cggcaatgac ttcacgttta
    60601 ctgctggatc tgacctacgg tgtactggcc gatatcgaag cggaagacct ggcgcgtgaa
    60661 gcgtcgtttg ctcagggatt acgcgcgatg attggcggta tcttaaccgc atcctgattc
    60721 tctctctctt tccgcgggct ggtgacaact gagcccgcgt ttcatacttt aatttccctg
    60781 tacaaaatac aatctcgccg gagcggggaa ttcctcccta aaatattcac cgtagccata
    60841 tgtcatgaga gtttatcgtt cccaacatgc tcgaacgaac gttcggttgc ttattttatg
    60901 gcttctgtca acgccgtttt aaagattaat gcgatctata tcacgctgtg ggtattgcag
    60961 tttttggttt tttgatcgcg gtgtcagttc tttttatttc catttctctt ccatgggttt
    61021 ctcacagata actgtgtgca acacagaatt ggttaactaa tcagattaaa ggttgaccag
    61081 tatttttatc ttaatgagga gtcccttatg ttacgtcctg tagaaacccc aacccgtgaa
    61141 atcgaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgag
    61201 cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt
    61261 aacgatcagt tcgccgatgc agatattcgt aattatgtgg gcaacgtctg gtatcagcgc
    61321 gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc
    61381 actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca gggcggctat
    61441 acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcaca
    61501 gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac
    61561 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactacgc cgggatccat
    61621 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
    61681 catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat
    61741 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggggcacca
    61801 gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg
    61861 aactgtacgt cacagccaaa agccggacag agtgtgatat ctacccgctg cgcgtcggca
    61921 tccggtcagt ggcagtgaag ggcgaacagt tcctgatcaa ccacaaaccg ttctacttta
    61981 ctggctttgg ccgtcatgaa gatgcggatt tgcgcggcaa aggattcgat aacgtgctga
    62041 tggtgcacga tcacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt
    62101 acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg
    62161 aaactgcagc tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc
    62221 cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaggcg cacttacagg
    62281 cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg
    62341 ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag
    62401 caacgcgtaa actcgatccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg
    62461 ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggtt
    62521 ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg
    62581 cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag
    62641 ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg
    62701 atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt
    62761 tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaggggatct
    62821 tcacccgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca
    62881 tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac tctcctggcg
    62941 caccatcgtc ggctacagcc ttggtgacat cgccaataac ttcgccttcg caatgggggc
    63001 gctcttcctg ttgagttact acaccgacgt cgctggcgtc ggtgccgctg cggcgggcac
    63061 catgctgtta ctggtgcggg tattcgatgc cttcgccgac gtctttgccg gacgagtggt
    63121 ggacagtgtg aatacccgct ggggaaaatt ccgcccgttt ttactcttcg gtactgcgcc
    63181 gttaatgatc ttcagcgtgc tggtattctg ggtgccgacc gactggagcc atggtagcaa
    63241 agtggtgtat gcatatttga cctacatggg cctcgggctt tgctacagcc tggtgaatat
    63301 tccttatggt tcacttgcta ccgcgatgac ccaacaacca caatcccgcg cccgtctggg
    63361 cgcggctcgt gggattgccg cttcattgac ctttgtctgc ctggcatttc tgatagggcc
    63421 gagcattaag aactccagcc cggaagagat ggtgtcggta taccatttct ggacgattgt
    63481 gctggcgatt gccggaatgg tgctttactt catctgcttc aaatcgacgc gtgagaatgt
    63541 ggtacgtatc gtggcgcagc cgtcattgaa gatcagtctg caaaccctga aacggaatcg
    63601 cccgctgttt atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct tcgcggtcag
    63661 cgcctcgtcg ttgttctacg tgcgctatgt gttaaatgat accgggctgt tcactgtgct
    63721 ggtactggtg caaaacctgg ttggtactgt ggcatcggca ccgttggtgc cggggatggt
    63781 cgcgaggatc ggtaaaaaga ataccttcct gattggcgct ttgctgggaa cctgcggtta
    63841 tctgctgttc ttctgggttt ccgtctggtc gctgccggtg gcgttggttg cgttagccat
    63901 tgcctcaatt ggtcagggcg ttaccatgac cgtgatgtgg gcgctggaag ctgataccgt
    63961 agaatacggt gaatacctga ccggcgtgcg aattgaaggg ctcacctatt cactattctc
    64021 atttacccgt aaatgcggtc aggcaatcgg tggttcaatt cctgccttta ttttgggatt
    64081 aagcggatat atcgccaatc aggtgcaaac gccggaagta attatgggca tccgcacatc
    64141 aattgcctta gtaccttgcg gatttatgct actggcattc gttattatct ggttttatcc
    64201 gctcacggat aaaaaattca aagaaatcgt ggttgaaatt gataatcgta aaaaagtgca
    64261 gcag
//
LOCUS       DAZGXS010000011        61764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGXS010000011 DAZGXS010000000
VERSION     DAZGXS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(170..1645)
                     /gene="ampG"
                     /locus_tag="ACHKG5_000836"
     CDS             complement(170..1645)
                     /gene="ampG"
                     /locus_tag="ACHKG5_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0866995.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1689..2267)
                     /gene="yajG"
                     /locus_tag="ACHKG5_000837"
     CDS             complement(1689..2267)
                     /gene="yajG"
                     /locus_tag="ACHKG5_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0866996.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2572..2889
                     /gene="bolA"
                     /locus_tag="ACHKG5_000838"
     CDS             2572..2889
                     /gene="bolA"
                     /locus_tag="ACHKG5_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0866997.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3233..4531
                     /gene="tig"
                     /locus_tag="ACHKG5_000839"
     CDS             3233..4531
                     /gene="tig"
                     /locus_tag="ACHKG5_000839"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0866998.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4776..5399
                     /gene="clpP"
                     /locus_tag="ACHKG5_000840"
     CDS             4776..5399
                     /gene="clpP"
                     /locus_tag="ACHKG5_000840"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0866999.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5525..6799
                     /gene="clpX"
                     /locus_tag="ACHKG5_000841"
     CDS             5525..6799
                     /gene="clpX"
                     /locus_tag="ACHKG5_000841"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0867000.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6987..9341
                     /gene="lon"
                     /locus_tag="ACHKG5_000842"
     CDS             6987..9341
                     /gene="lon"
                     /locus_tag="ACHKG5_000842"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0867001.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9532..9822
                     /gene="hupB"
                     /locus_tag="ACHKG5_000843"
     CDS             9532..9822
                     /gene="hupB"
                     /locus_tag="ACHKG5_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0867002.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10014..11885
                     /gene="ppiD"
                     /locus_tag="ACHKG5_000844"
     CDS             10014..11885
                     /gene="ppiD"
                     /locus_tag="ACHKG5_000844"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0867003.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12036..12407
                     /gene="ybaV"
                     /locus_tag="ACHKG5_000845"
     CDS             12036..12407
                     /gene="ybaV"
                     /locus_tag="ACHKG5_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0867004.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12513..12911
                     /gene="fadM"
                     /locus_tag="ACHKG5_000846"
     CDS             12513..12911
                     /gene="fadM"
                     /locus_tag="ACHKG5_000846"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0867005.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12963..13658)
                     /gene="queC"
                     /locus_tag="ACHKG5_000847"
     CDS             complement(12963..13658)
                     /gene="queC"
                     /locus_tag="ACHKG5_000847"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0867006.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13723..15423)
                     /gene="ybaE"
                     /locus_tag="ACHKG5_000848"
     CDS             complement(13723..15423)
                     /gene="ybaE"
                     /locus_tag="ACHKG5_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0867007.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15523..16341
                     /gene="cof"
                     /locus_tag="ACHKG5_000849"
     CDS             15523..16341
                     /gene="cof"
                     /locus_tag="ACHKG5_000849"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0867008.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16493..16951
                     /gene="decR"
                     /locus_tag="ACHKG5_000850"
     CDS             16493..16951
                     /gene="decR"
                     /locus_tag="ACHKG5_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0867009.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16981..18753
                     /gene="mdlA"
                     /locus_tag="ACHKG5_000851"
     CDS             16981..18753
                     /gene="mdlA"
                     /locus_tag="ACHKG5_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0867010.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18746..20527
                     /gene="mdlB"
                     /locus_tag="ACHKG5_000852"
     CDS             18746..20527
                     /gene="mdlB"
                     /locus_tag="ACHKG5_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0867011.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20708..21046
                     /gene="glnK"
                     /locus_tag="ACHKG5_000853"
     CDS             20708..21046
                     /gene="glnK"
                     /locus_tag="ACHKG5_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0867012.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21076..22362
                     /gene="amtB"
                     /locus_tag="ACHKG5_000854"
     CDS             21076..22362
                     /gene="amtB"
                     /locus_tag="ACHKG5_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0867013.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22411..23271)
                     /gene="tesB"
                     /locus_tag="ACHKG5_000855"
     CDS             complement(22411..23271)
                     /gene="tesB"
                     /locus_tag="ACHKG5_000855"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0867014.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23489..24061
                     /gene="ybaY"
                     /locus_tag="ACHKG5_000856"
     CDS             23489..24061
                     /gene="ybaY"
                     /locus_tag="ACHKG5_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0867015.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24094..24405)
                     /locus_tag="ACHKG5_000857"
     CDS             complement(24094..24405)
                     /locus_tag="ACHKG5_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0867016.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24568..24664
                     /gene="ffs"
                     /locus_tag="ACHKG5_000858"
     ncRNA           24568..24664
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKG5_000858"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24784..25137
                     /gene="ybaA"
                     /locus_tag="ACHKG5_000859"
     CDS             24784..25137
                     /gene="ybaA"
                     /locus_tag="ACHKG5_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP0867017.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25179..26729)
                     /gene="pdeB"
                     /locus_tag="ACHKG5_000860"
     CDS             complement(25179..26729)
                     /gene="pdeB"
                     /locus_tag="ACHKG5_000860"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0867018.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26893..27363)
                     /gene="ylaC"
                     /locus_tag="ACHKG5_000861"
     CDS             complement(26893..27363)
                     /gene="ylaC"
                     /locus_tag="ACHKG5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0867019.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27479..28030)
                     /gene="maa"
                     /locus_tag="ACHKG5_000862"
     CDS             complement(27479..28030)
                     /gene="maa"
                     /locus_tag="ACHKG5_000862"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP0867020.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28202..28420)
                     /gene="hha"
                     /locus_tag="ACHKG5_000863"
     CDS             complement(28202..28420)
                     /gene="hha"
                     /locus_tag="ACHKG5_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0867021.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28446..28820)
                     /gene="tomB"
                     /locus_tag="ACHKG5_000864"
     CDS             complement(28446..28820)
                     /gene="tomB"
                     /locus_tag="ACHKG5_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0867022.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29366..32515)
                     /gene="acrB"
                     /locus_tag="ACHKG5_000865"
     CDS             complement(29366..32515)
                     /gene="acrB"
                     /locus_tag="ACHKG5_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0867023.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(32538..33731)
                     /gene="acrA"
                     /locus_tag="ACHKG5_000866"
     CDS             complement(32538..33731)
                     /gene="acrA"
                     /locus_tag="ACHKG5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0867024.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33873..34520
                     /gene="acrR"
                     /locus_tag="ACHKG5_000867"
     CDS             33873..34520
                     /gene="acrR"
                     /locus_tag="ACHKG5_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0867025.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34648..38010
                     /gene="mscK"
                     /locus_tag="ACHKG5_000868"
     CDS             34648..38010
                     /gene="mscK"
                     /locus_tag="ACHKG5_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0867026.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38049..38213)
                     /gene="rsmS"
                     /locus_tag="ACHKG5_000869"
     CDS             complement(38049..38213)
                     /gene="rsmS"
                     /locus_tag="ACHKG5_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0867027.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(38227..38754)
                     /gene="priC"
                     /locus_tag="ACHKG5_000870"
     CDS             complement(38227..38754)
                     /gene="priC"
                     /locus_tag="ACHKG5_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0867028.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38824..39201
                     /gene="ybaN"
                     /locus_tag="ACHKG5_000871"
     CDS             38824..39201
                     /gene="ybaN"
                     /locus_tag="ACHKG5_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0867029.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39354..39905
                     /gene="apt"
                     /locus_tag="ACHKG5_000872"
     CDS             39354..39905
                     /gene="apt"
                     /locus_tag="ACHKG5_000872"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0867030.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40034..41965
                     /gene="dnaX"
                     /locus_tag="ACHKG5_000873"
     CDS             40034..41965
                     /gene="dnaX"
                     /locus_tag="ACHKG5_000873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0867031.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    41298..41362
                     /gene="dnaX"
                     /locus_tag="ACHKG5_000873"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42018..42347
                     /gene="ybaB"
                     /locus_tag="ACHKG5_000874"
     CDS             42018..42347
                     /gene="ybaB"
                     /locus_tag="ACHKG5_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0867032.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42347..42952
                     /gene="recR"
                     /locus_tag="ACHKG5_000875"
     CDS             42347..42952
                     /gene="recR"
                     /locus_tag="ACHKG5_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0867033.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43062..44936
                     /gene="htpG"
                     /locus_tag="ACHKG5_000876"
     CDS             43062..44936
                     /gene="htpG"
                     /locus_tag="ACHKG5_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0867034.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45117..45761
                     /gene="adk"
                     /locus_tag="ACHKG5_000877"
     CDS             45117..45761
                     /gene="adk"
                     /locus_tag="ACHKG5_000877"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0867035.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            45893..46855
                     /gene="hemH"
                     /locus_tag="ACHKG5_000878"
     CDS             45893..46855
                     /gene="hemH"
                     /locus_tag="ACHKG5_000878"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0867036.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(46852..47811)
                     /gene="aes"
                     /locus_tag="ACHKG5_000879"
     CDS             complement(46852..47811)
                     /gene="aes"
                     /locus_tag="ACHKG5_000879"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0867037.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            47963..49267
                     /gene="gsk"
                     /locus_tag="ACHKG5_000880"
     CDS             47963..49267
                     /gene="gsk"
                     /locus_tag="ACHKG5_000880"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0867038.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49400..51076)
                     /gene="ybaL"
                     /locus_tag="ACHKG5_000881"
     CDS             complement(49400..51076)
                     /gene="ybaL"
                     /locus_tag="ACHKG5_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0867039.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51313..52533)
                     /gene="fsr"
                     /locus_tag="ACHKG5_000882"
     CDS             complement(51313..52533)
                     /gene="fsr"
                     /locus_tag="ACHKG5_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0867040.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52751..54403
                     /gene="ushA"
                     /locus_tag="ACHKG5_000883"
     CDS             52751..54403
                     /gene="ushA"
                     /locus_tag="ACHKG5_000883"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0867041.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54440..54919)
                     /gene="ybaK"
                     /locus_tag="ACHKG5_000884"
     CDS             complement(54440..54919)
                     /gene="ybaK"
                     /locus_tag="ACHKG5_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0867042.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55123..55917)
                     /gene="ybaP"
                     /locus_tag="ACHKG5_000885"
     CDS             complement(55123..55917)
                     /gene="ybaP"
                     /locus_tag="ACHKG5_000885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0867043.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56055..56396
                     /gene="ybaQ"
                     /locus_tag="ACHKG5_000886"
     CDS             56055..56396
                     /gene="ybaQ"
                     /locus_tag="ACHKG5_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0867044.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56611..59115)
                     /gene="copA"
                     /locus_tag="ACHKG5_000887"
     CDS             complement(56611..59115)
                     /gene="copA"
                     /locus_tag="ACHKG5_000887"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0867045.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59377..60309
                     /gene="glsA"
                     /locus_tag="ACHKG5_000888"
     CDS             59377..60309
                     /gene="glsA"
                     /locus_tag="ACHKG5_000888"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0867046.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60312..61604
                     /gene="ybaT"
                     /locus_tag="ACHKG5_000889"
     CDS             60312..61604
                     /gene="ybaT"
                     /locus_tag="ACHKG5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0867047.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT        15262 a        15578 c        16399 g        14525 t
ORIGIN      
        1 taaagactca ttagctgaat cgtgtaagaa aaatagaatt tataaatggc gccaataatt
       61 tccaaacgct aagccgcaca aaagaacaaa tattaataag cgatgctgat gtttatttag
      121 ccgtaataat tacggcgagt gattactaca gctaaataat atttacagat tacgtcagat
      181 gcgtttttcg tagcgccaga taatccagca aaccaccaag cacgacgcca gaaagggcga
      241 ctaaaacccc gacttccagc agagcaggca ggaaagagaa gtgcgtcaaa tccagcgcgt
      301 ccatcgtcaa tagcagtaac cacacggcca acaggctgac gcccgccgcc agtgtccaca
      361 tggcaaaggc ataacctgcc ggatatgcgg tacgggagat aaagttgtca tttactcgtg
      421 tatattcaag cgtctggcgg caaaccagca gcaaaataag ccctggtacg gcagcggcga
      481 cggagaatag atagaatgtc gaccagccgt gtgcttcaac aaaccaaccc gccacggggc
      541 cgacataaac tcgccctaca gcagaaagcg ctgagagcag ggcaaattga gtagcggaaa
      601 atgacttatt acatagcgtc attaacagcg cgacaaaggc tgatgtgccc atcccgccac
      661 agaggttttc gaaaaagacg gctgcgccca tgctgtagag atgcttatca gtaatcgaca
      721 gcagccagta accagcgtta gacgcacctt gtaaaatgcc gaaaatcagc agtgcccgga
      781 acagtgacag gcgctgcatc aaaatcccac cgtacaatgc gccaacaatg gtcgctagta
      841 agccaagcgt tttgtttacc acgcctactt cacccgcatc aaacccgacg ccgcgaatca
      901 aaaacgtggt tgtcaggctc atggcgaatg cgtcgcccag cttatacagc acgataagaa
      961 gcaaaataag ccaggcatta ttgcgaccaa agaaatctcg cagaggtgca acaaccgctt
     1021 gttccagcgt tttgggcaca ggaatggtgt cggttggttc tggtgcaagc aacgtcgcga
     1081 taatacaggg gatcaacagc gccgccatca accagtacat gccctgccag cccagccatt
     1141 tatctgccag ccacagggct aggccgccgg aaaccagcat ccctaaacgg taacccagaa
     1201 cgctgattgc cgcgcccgca ccacgttctt ctgccgggag cacatcggtt ttccacgcat
     1261 cgaagacaat atcctgggag gcagagcaaa aagcgatcac caccgccagc gccgccatcc
     1321 agcggagttg ggtgcctggt tcgagaaaac ccatcgccgc aatggcgact aataacagga
     1381 tttgcgtggc gagcagccaa ccgcgccgcc gcccaaaaaa tgggggcgtg tagcggtcca
     1441 tcagcggtga ccagaggaat ttaaaaacgt aagcctggcc taccagagag aagaaaccaa
     1501 tggttttgag atcgatattc tcgaccgtca tccaggcctg taaggtgccg gatgtcaggg
     1561 cgagcggtag cccggaagca aaacccagga tcagcaatat ggctgaacgc ggctgttgaa
     1621 aaatacgtaa atattgactg gacatgggcg tctacaggcc cggcttgcac cgggccagag
     1681 ggcagaaatt aacgcgcgtt ctgcttgatg aattcgtgaa tgctggtgtc ctgagacata
     1741 tcagcgatgg tatcggtcag cacactgtta accgcatcgg cgatattttt gttggaggcc
     1801 tggaacgcac cttcaacgtt gtagctggca cgatagtttt tggtcatttt gttgccattc
     1861 tgcgcggtag cgatgatggc gatatccgct ttggtagcga tgttgtagcg cacgttgccc
     1921 tgggacacgt cagcatacag ctggctaacg atgatttgca gattaaccgg gccattcgga
     1981 ccaaccatgt aaccacgcgc ggtcatctgt ttttccagca cttcttgcag caggaaacgc
     2041 agatcgcggg aggcggtcag ggtaacgatt tgattatcgc gggtgacttt tgccagcgcc
     2101 tgatcggtac gctgatcggc accattaatg cttacggtga cgcccatcag gcttggatcc
     2161 tgctgtggca gtgtaatcgt cggggaaact tcaatagttg ttggcggtct tgcgcatcct
     2221 gcaagcataa acagagcaac taacgggaag aggatttttt tgaacatgtt cgggctctca
     2281 gagactctta agcgtgtttg gtaaaaattc ccgccatcat aacattgcca acggcgaggg
     2341 gaagtaggta aggcatgtaa attcatcatg ttgacgaaat aatcgcccct ggtaaaagaa
     2401 acgctgatgc gaggcctgtg tttcaatctt taaatcagta aacttcatac gcttgacgga
     2461 aaaaccagga cgaaacctaa atatttgttg ttaagctgca atggaaacgg taaaagcggc
     2521 tagtatttaa agggatggat gacatctcag cgttgtcgga ggagatattt catgatgata
     2581 cgtgagcgga tagaagaaaa attaagggcg gcgttccaac ccgtattcct cgaagtagtg
     2641 gatgaaagct atcgtcacaa tgtcccagcc ggctctgaaa gccattttaa agttgtgctg
     2701 gtcagcgatc gttttacggg tgaacgtttt ctgaatcgtc atcgaatgat ttacagtact
     2761 ttagcggagg aactctctac taccgttcat gcgctggctc tgcatactta cactattaag
     2821 gagtgggaag ggttgcagga caccgtcttt gcctctcctc cctgtcgtgg agcaggaagc
     2881 atcgcgtaaa aacgcatttg caactgtcgg cgcttttcca gtatgttgct aaagatttta
     2941 tgaaaaacgg cctgcgggcc gttttgtttt gtctggattt tgcgcttttt gcccagcatt
     3001 cagacgaaaa ttgcccggga attgtgaaaa aatacgcgac agcgcgcaat aaccgttctc
     3061 gactcataaa agtgatgccg ctataatgcc gcgtcctatt tgaatgcttt cgggatgatt
     3121 ctggtaacag ggaatgtgat tgattataag aacatcccgg ttccgcgaag ccaacaacct
     3181 gtgcttgcgg ggtaagagtt gaccgagcac tgtgattttt tgaggtaaca agatgcaagt
     3241 ttcagttgaa accactcaag gccttggccg ccgtgtaacg attactatcg ctgctgacag
     3301 catcgagacc gctgttaaaa gcgagctggt caacgttgcg aaaaaagtac gtattgacgg
     3361 cttccgcaaa ggcaaagtgc caatgaatat cgttgctcag cgttatggcg cgtctgtacg
     3421 ccaggacgtt ctgggtgacc tgatgagccg taacttcatt gacgccatca ttaaagaaaa
     3481 aatcaatccg gctggcgcac cgacttatgt tccgggcgaa tacaagctgg gtgaagactt
     3541 cacctactct gtagagtttg aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat
     3601 cgaagttgaa aaaccgatcg ttgaagtgac cgacgctgac gttgacggca tgctggatac
     3661 tctgcgtaaa cagcaggcga cctggaaaga aaaagacggc gctgttgaag cagaagaccg
     3721 cgtaaccatc gacttcaccg gttctgtaga cggcgaagag ttcgaaggcg gtaaagcgtc
     3781 tgatttcgta ctggcgatgg gccagggtcg tatgatcccg ggctttgaag acggtatcaa
     3841 aggccacaaa gctggcgaag agttcaccat cgacgtgacc ttcccggaag aataccacgc
     3901 agaaaacctg aaaggtaaag cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg
     3961 tgaactgccg gaactgactg cagaattcat caaacgtttc ggcgttgaag atggttccgt
     4021 agaaggtctg cgcgctgaag tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg
     4081 taaccgcgtt aagtctcagg cgatcgaagg tctggtaaaa gctaacgaca tcgatgtacc
     4141 ggctgcgctt attgacagcg aaatcgacgt tctgcgtcgc caggctgcac agcgtttcgg
     4201 tggcaacgaa aaacaagctc tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg
     4261 ccgcgtagtt gttggcctgc tgctgggcga agttatccgc accaacgagc tgaaagctga
     4321 cgaagagcgc gtgaaaggcc tgatcgaaga gatggcttca gcgtacgaag atccgaaaga
     4381 agttatcgag ttctacagca aaaacaaaga actgatggac aacatgcgca atgttgctct
     4441 ggaagaacag gctgttgaag ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac
     4501 tttcaacgag ctgatgaacc agcaggcgta atttacgcag cataacgcgc taaattcgca
     4561 caaaggcccg tcaccgccag gtggtgggct ttttttgtca tgaattttgc atggaaccgt
     4621 gcgaaaagcc tctttcggtg ttagcgtaac aacaaaagat tgttatgctt gaaatatggt
     4681 gatgccgtac ccataacaca gggactagct gataatccgt ccataaggtt acaatcggta
     4741 cagcaggttt tttcaatttt atccaggaga cggaaatgtc atacagcggc gaacgagata
     4801 actttgcacc ccatatggcg ctggtgccga tggtcattga acagacctca cgtggtgagc
     4861 gctcttttga tatctattct cgtctactta aggaacgcgt catttttctg actggccagg
     4921 ttgaagacca tatggctaac ctgattgtgg ctcagatgct gttcctggaa gcagaaaacc
     4981 cagaaaaaga tatctatctg tacattaact ccccaggcgg ggtgatcact gccgggatgt
     5041 ctatctatga caccatgcag tttatcaagc ctgatgtcag caccatctgt atgggccagg
     5101 cggcctcgat gggcgctttc ttgctgaccg caggggcaaa aggtaaacgt ttttgcctgc
     5161 cgaattcgcg cgtgatgatt caccaaccgt tgggcggcta ccagggccag gcgaccgata
     5221 tcgaaattca tgcccgtgaa attctgaaag ttaaagggcg tatgaatgaa cttatggcgc
     5281 ttcatacggg tcaatcatta gaacagattg aacgtgatac cgagcgcgat cgcttccttt
     5341 ccgcccctga agcggtggaa tacggtctgg tcgattcgat tctgacccat cgtaattgat
     5401 gccagaggcg caactgtgcc gctatactta tccagggcgg cacaacgctg taagcggctt
     5461 gcgcctgaga atggcatttg cgtcgtcgtg tgcggcacaa agaacaaaga agaggttttg
     5521 acccatgaca gataaacgca aagatggctc aggcaaattg ctgtattgct ctttttgcgg
     5581 caaaagccag catgaagtgc gcaagctgat tgccggtcca tccgtgtata tctgcgacga
     5641 atgtgttgat ttatgtaacg acatcattcg cgaagagatt aaagaagttg caccgcatcg
     5701 tgaacgcagt gcgctaccga cgccgcatga aattcgtaac cacctggacg attacgttat
     5761 cggccaggaa caggcgaaaa aagtgctggc ggtcgcggta tacaaccact acaaacgtct
     5821 gcgcaacggc gataccagca atggcgtcga gttgggcaaa agtaacattc tgctgatcgg
     5881 tccgaccggt tccggtaaaa cgctgctggc tgaaacgctg gcgcgcctgc tggacgtccc
     5941 gttcaccatg gccgacgcaa ccacgctgac cgaagccggt tatgtgggcg aagacgttga
     6001 aaacatcatt cagaagctgt tgcaaaagtg cgattacgac gtacagaaag cgcagcgcgg
     6061 gattgtctac atcgatgaaa tcgacaagat ttctcgtaag tcagacaacc cgtctattac
     6121 ccgtgacgtt tccggtgaag gcgtacagca ggcactgttg aaactgatcg aaggtacggt
     6181 agctgctgtt ccaccgcaag gtggacgtaa acatccgcag caggaattct tgcaggttga
     6241 tacctctaag atcctgttta tttgtggcgg tgcgtttgcc ggtctggata aagtgatttc
     6301 ccatcgtgta gaaaccggct ccggcattgg ttttggcgcg acggtaaaag cgaagtccga
     6361 caaagcaagc gaaggcgagc tgctggcgca ggttgaaccg gaagatctga tcaagtttgg
     6421 tcttatccct gagtttattg gtcgtctgcc ggttgtcgca acgttgaatg aactgagcga
     6481 agaagctctg attcagatcc tcaaagagcc gaaaaacgcc ctgaccaagc agtatcaggc
     6541 gctgtttaat ctggaaggcg tggatctgga attccgtgac gaggcgctgg atgctatcgc
     6601 taagaaagcg atggcgcgta aaaccggtgc ccgtggcctg cgttccatcg tagaagccgc
     6661 actgctcgat accatgtacg atctgccgtc catggaagat gtcgaaaaag tggttatcga
     6721 cgagtcggta attgatggtc aaagcaaacc gttgctgatt tatggcaagc cggaagcgca
     6781 acaggcatct ggtgaataat taaccattcc catacaatta gttaaccaaa aaggggggat
     6841 tttatctccc ctttaatttt tcctctattc tcggcgttga atgtggggga aacatcccca
     6901 tatactgacg tacatgttaa tagatggcgt gaagcacagt cgtgtcatct gattacctgg
     6961 cggaaattaa actaagagag agctctatga atcctgagcg ttctgaacgc attgaaatcc
     7021 ccgtattgcc gctgcgcgat gtggtggttt atccgcacat ggtcatcccc ttatttgtcg
     7081 ggcgggaaaa atctatccgt tgtctggaag cggcgatgga ccatgataaa aaaattatgc
     7141 tggtcgcgca gaaagaagct tcaacggatg agccgggtgt aaacgatctt ttcaccgtcg
     7201 ggaccgtggc ctctatattg cagatgctga aactgcctga cggcaccgtc aaagtgctgg
     7261 tcgaggggtt acagcgcgcg cgtatttctg cgctctctga caatggcgaa cacttttctg
     7321 cgaaggcgga gtatctggag tcgccgacca ttgatgagcg agaacaggaa gtgctggtgc
     7381 gtactgcaat cagccagttc gaaggctaca tcaagctgaa caaaaaaatc ccaccagaag
     7441 tgctgacgtc gttgaatagc atcgacgatc cggcgcgtct ggcggatacc attgctgcac
     7501 atatgccgct gaaactggct gacaaacagt ccgttctgga gatgtccgac gttaacgaac
     7561 gtctggaata tctgatggca atgatggaat cggaaatcga tctgctgcag gttgagaaac
     7621 gcattcgcaa ccgcgttaaa aagcagatgg agaaatccca gcgtgagtac tatctgaacg
     7681 agcaaatgaa agctattcag aaagaactcg gtgaaatgga cgacgcgccg gacgaaaacg
     7741 aagccctgaa gcgcaaaatc gacgcggcga agatgccgaa agaggcaaaa gagaaagcgg
     7801 aagcagagtt gcagaagctg aaaatgatgt ctccgatgtc ggcagaagcg accgtagtgc
     7861 gtggttatat cgactggatg gtacaggtac cgtggaatgc gcgcagcaag gtcaaaaaag
     7921 acctgcgtca ggcgcaggaa atccttaata ccgaccatta tggtctggag cgcgtgaaag
     7981 atcgcatcct tgagtatctt gcggttcaaa gccgtgtcaa caaaatcaag ggaccgatcc
     8041 tttgcctggt agggccgccg ggggtaggta aaacctccct gggtcagtcc attgccaaag
     8101 ccaccgggcg taaatatgtc cgtatggcgc tgggcggcgt gcgtgatgaa gcggaaatcc
     8161 gtggtcaccg ccgtacttac atcggttcta tgccgggtaa attgatccag aaaatggcga
     8221 aagtgggcgt taaaaacccg ctgttcctgc tcgatgagat cgacaaaatg tcttctgaca
     8281 tgcgtggcga tccggcttcc gcactgcttg aagtgctgga tccagagcag aacgtggcct
     8341 tcagcgatca ctacctggaa gtggattacg atctcagcga cgtgatgttt gtcgcgacgt
     8401 cgaactccat gaacattccg gcaccactgc tggatcgtat ggaagtgatt cgcctctccg
     8461 gttataccga agatgaaaaa ctgaacatcg ccaaacgtca cctgctgccg aagcagattg
     8521 aacgtaatgc actgaaaaaa ggtgagctga ccgtcgacga tagcgccatt atcggcatta
     8581 ttcgttacta cacccgtgag gcgggcgtgc gtggtctgga gcgtgaaatc tccaagctgt
     8641 gccgtaaagc ggttaagcag ttactgctcg ataagtcatt aaaacatatc gaaattaacg
     8701 gcgacaacct gcatgactac cttggtgttc agcgtttcga ctatggtcgc gctgataacg
     8761 aaaaccgtgt cggtcaggta accggtctgg cgtggacgga agtgggcggt gacttgctga
     8821 ccattgaaac cgcatgtgtt ccgggtaaag gcaaactgac ctataccggt tcgctcggcg
     8881 aagtgatgca ggagtctatt caggctgcgt taacggtggt tcgcgcgcgt gcggaaaaac
     8941 tggggatcaa ccctgatttt tacgaaaaac gtgacatcca cgtccacgta ccggaaggtg
     9001 cgacgccgaa agatggtccg agtgccggta ttgctatgtg caccgcgctg gtttcttgcc
     9061 tgaccggtaa cccggttcgt gccgatgtgg caatgaccgg tgagatcact ctgcgtggtc
     9121 aggtactgcc gatcggtggt ttgaaagaaa aactactggc agcgcatcgc ggcgggatta
     9181 aaacagtgtt aattccgttc gaaaataaac gcgatctgga agagattcct gacaacgtaa
     9241 ttgccgatct ggacattcat cctgtgaagc gcattgagga agttctgact ctggcgctgc
     9301 aaaatgaacc gtctggcatg caggttgtga ctgcaaaata gtgacctcgc gcaaaatgca
     9361 ctaataaaaa cagggctggc aggctaattc gggcttgcca gccttttttt gtctcgctaa
     9421 gttagatggc ggatcgggct tgcccttatt aaggggtgtt gtaaggggat ggctggcctg
     9481 atataactgc tgcgcgttcg taccttgaag gattcaagtg cgatataaat tataaagagg
     9541 aagagaagag tgaataaatc tcaattgatc gacaagattg ctgcaggggc tgatatctct
     9601 aaagctgcgg ctggccgtgc gttagatgct attattgctt ccgtaactga atctctgaaa
     9661 gaaggggatg atgtagcact ggtaggtttt ggtacttttg ccgttaaaga gcgtgctgcc
     9721 cgtactggcc gcaacccgca gaccggtaaa gagatcacca tcgctgccgc taaagtaccg
     9781 agcttccgtg caggtaaagc actgaaagac gcggtaaact aagcgttgtc cccagtgggg
     9841 atgtgacgaa gttcaagggc gcatctactg atgtgccttt tttatttgta ttcggtgact
     9901 ttctgcgtct tgtgggctga caattgcccc cgtttcttgt cacaataggc ctttgcgcgc
     9961 atcgatacgt tgcgtgaggt acacagtcat ctacagcgga gtgttgttac accatgatgg
    10021 acagcttacg cacggctgca aacagtctcg tgctcaagat tattttcggt atcattatcg
    10081 tgtcgttcat attgaccggc gtgagtggtt acctgattgg cggaggcaat aactacgccg
    10141 caaaagtgaa tgaccaggaa atcagccgtg ggcaattcga gaatgccttc aacagcgagc
    10201 gtaatcgcat gcagcaacag ctgggcgatc aatactccga gctggcagcg aacgaaggct
    10261 atatgaaaac cctgcgtcaa caggtgctga atcgtctgat cgacgaggcg ctgctggatc
    10321 agtacgcacg tgagctgaaa ctgggtatca gcgatgagca ggttaaacag gcgattttcg
    10381 cgaccccagc cttccaggtt gatggcaaat ttgataacag ccgctataac ggtatcctca
    10441 accagatggg gatgaccgcc gatcagtacg cccaggcgct gcgtaaccag ctcactaccc
    10501 aacagctgat taacggcgtt gctggtaccg attttatgct gaaaggtgaa accgacgagc
    10561 tggcggcact ggtcgctcaa caacgcgtgg tgcgtgaagc gactatcgat gttaacgcgc
    10621 tggcggcgaa gcagcctgtg accgaacagg aaattgccag ctactacgaa caaaacaaaa
    10681 acaatttcat gacgccggaa caattccgcg tgagttacat caagctggat gccgcaacga
    10741 tgcagcaacc ggttagcgat gcggatctcc agagctacta cgatcagcat caggatcaat
    10801 tcacccagcc gcagcgtacc cgttacagca tcatccagac caaaactgaa gatgaagcga
    10861 aagcggtact tgatgagctg aataaaggcg gtgattttgc tgcattagcc aaagaaaaat
    10921 ctgccgatat tatctctgct cgtaacggcg gcgatatggg ttggttagaa gatgccacta
    10981 tcccggatga actgaaaaat gctggtctga aagaaaaagg ccaactgtct ggtgtcatca
    11041 aatcttcggt cggtttcctg attgtacgtc tggacgacat tcagccagcg aaagtgaaat
    11101 cgttagacga agtacgtgac gatgtcgcgg cgaaagtgaa acacgaaaaa gccctcgatg
    11161 cgtactacgc gctgcagcag aaagtgagcg atgcggcaag caacgacacc gagtctctgg
    11221 ccggtgcaga gcaagctgcc ggcgttaaag ccactcagac gggttggttc agcaaagaga
    11281 acctgccgga agagttgaac ttcaagccgg ttgctgacgc tatctttaac ggcggtctgg
    11341 taggtgaaaa cggcgcgccg ggcatcaact ctgacatcat caccgtagac ggcgaccgcg
    11401 cattcgtgct gcgcatcagc gagcacaaac cggaagcggt gaaaccgttg gcagatgttc
    11461 aggaacaagt taaggcactg gttcagcaca acaaagctga acaacaggcg aaagtggatg
    11521 ctgagaaact gctggttgat ttgaaagccg gcaaaggtgc ggaagctatg caggctgccg
    11581 gtctgaaatt tggcgagccg aaaaccttaa gccgttccgg tcgtgacccg attagccagg
    11641 cggcgtttgc actgccactg ccagcgaaag acaaaccgag ctacggtatg gcgaccgata
    11701 tgcaaggtaa tgtggttctg ctggcgctgg atgaagtgaa acaaggttca atgccggaag
    11761 atcagaaaaa agcgatggtg cagggtatca cccagaacaa cgcacaaatc gtctttgaag
    11821 ctctgatgag taacctgcgt aaagaggcga aaatcaaaat tggcgatgcg ttggaacagc
    11881 aataatcctg aagccgcctc gcaaaaaaat gctttgcagc tgtaacaaga aaaggtcgct
    11941 ttcgcggcct tttccatttc tgaacattgc catttgttta ctgttttcac tgccgttaag
    12001 gtgattccac tgttaacaaa caaggagaaa acagtatgaa acacggaatt aaagcactgc
    12061 tcattaccct gtccctggcc tgtgccggaa tgtctcatag cgcgctggcg gcagcttctg
    12121 tggcgaaacc gacggcggta gaaaccaaag cggaagctcc tgcagcacaa aataaagcag
    12181 cagtaccggc gaaagccagt gacgaagaag gctcccgggt cagcattaat aatgccagcg
    12241 cggaagagct agcccgcgcg atgaatggcg ttggcctgaa gaaagcgcaa gcgattgtca
    12301 gttatcgcga agagtacggt ccgtttaaaa cggtggagga tctaaagcag gtgccgggga
    12361 tgggcaattc gctggtggaa cgtaatctgg cggtattaac cctgtaatta atttgcatag
    12421 tggcaatttt ttgccagact gaagaggtca taccagttat gacctctgta cttataacag
    12481 tttttataaa aacaacgtaa ggttattgcg ctatgcaaac acaaatcaaa gttcgtggat
    12541 atcatctcga cgtttaccag cacgtaaaca acgcccgcta ccttgaattt ctcgaagaag
    12601 cccgctggga tgggttggaa aatagcgaca gttttcactg gatgacggcc cataacatcg
    12661 ctttcgtggt ggtgaatatc aatattaact atcgtcgtcc ggcggtatta agcgacttgt
    12721 taaccattac gagccagtta cagcaattaa acggtaaaag cggcatctta agccaggtca
    12781 ttacactgga gccggaaggg caggtggtag cggatgcgct tattacgttt gtttgtattg
    12841 atcttaaaac gcagaaagca ttagctctgg aaggggaatt gcgcgaaaag ctggagcaga
    12901 tggttaagta aacgttttgt ggtgccggat gctcaagccg catccggcga cacccggaat
    12961 aattacttca acccggtttt ctgcttcatc gctgccatca ccgtcggttt atcggccaga
    13021 taatgattca aaccgttggc gcgtaaatta catgccgcac aatgaccgca accgtcgcct
    13081 ttaatgccgt tatagcaggt caacgtttcg ttacggacta aatccagttt gccgtaataa
    13141 tctgccagcg cccaggtttc cgctttatca atccacatca gcggcgtttc aaaacgaata
    13201 tctttcgcca tgcccaaact gacggcatgg tttagtactt tcacaaactc atcgcggcaa
    13261 tccgggtagc cggagaaatc cgtttcgcag acgccggtaa ttacggcttc tgcttttacc
    13321 tgatacgcat atattgccgc cagcgtcagg aacaaaatat tacgccctgg gacaaacgta
    13381 ttcgggatgc catcggcttc aggttcataa tcaggcaccg gaatgctgtc acgcgtcagg
    13441 ctgctgaccg ccagctcgtt gagcagcgtt acatcaagaa ccttatgcgc gcgtgccccc
    13501 agtttcagcg ccagttcgcg tgccacgtcg atttctgcgc gatggcgctg accgtaatcg
    13561 aacgtcacgc aatggacttc atcatattgt tgtaatgcct gcaccagaca ggtggtggaa
    13621 tcctggcctc cactgaacac aacgacagca cgtttcatag ataatcctgc ttgaacaata
    13681 aaagcgttat ggtaacgcct gcgattaacc cggaccagct attcattgcg acggcgcggg
    13741 aagccaggct tcggtaaatt caaaccagcc gcgcggtgtc agtcgcacac cgttcacgcc
    13801 gggaggggca ctaatgcgat agtgatagtt gaacagcggc gtcagcgtcg catctgtcat
    13861 taactggcta aaaaccgctt tcagggcatt aaatcggttt tcctcatcag gcattacttg
    13921 caccgcgtcc agtgtcgatt gtagatgtgc gtatgctgga gcgtcgaaaa catgtggcca
    13981 cagcggatcg cagcgcagcc attgctccag agtatattcc ggtgcttcgc caattaatct
    14041 gtcgcccatc atgaggtctg cgtgtgccag tagggtcgtg tcgtcccagt tttttgcgtt
    14101 atgaaaaata attgtgagtt cacagccttc cgctgccagt gtcgcctgta ggcgttctgc
    14161 catggtatga agttctatcg ggaggtgata aaccagcgtc aaggttttcg gtagtttgac
    14221 ttcatccggt acttgccaat gcggaatagt ccagcctggc agtaatgcat gactggcggt
    14281 gatcaggttt tctccgactt ctaacgtttg taataaaccg gattgatgaa taatggagat
    14341 cacttttcgc gcctgccaga gggagagtcg gggacttttg cgcaacgtta aatagcaaaa
    14401 acctaaactg atgccactac tgacctggct gacccgttgc agctcctccg gtttgccgat
    14461 ggtgatttgc acgggatgcc gacaactggt tcccaaatct ttttcgaaaa gcggcggagt
    14521 tatccagtac tcaaccgctt taagcagcgg atgacgtaaa tggtaataat catggctttc
    14581 caggcgcacc agttcagctg tgaattgtgt taagcgaaaa ggacccgtgc caatcagtgg
    14641 gaattgcgga tgcgccagat ggctgcaata gctcgccagc cggtgcgcaa gccagtaatc
    14701 ggggcgatgt aaaaagaagg tcagacactg cggatgggtg acctcaatac gcttcacgct
    14761 aataaataat tgatccagtg ctggcagttg taacagcatc aataatcgct ggtgtaagtg
    14821 tgaggctttt actgcatcgc cgttatgcca gtgtagggtt gaacgaagat aaaagtccca
    14881 gcgtaacccg tcagcagagg tttcccagtg atgcgctaaa tcgccaatcg ggcgctgggt
    14941 attattatcg aagcgggtca ggccggaaaa tatctgtccg gcgagatgct gctcggcacg
    15001 gccgggcaaa aagcccggtt gtagcggttc gagcgggcga tagtagggaa tacgcaacgt
    15061 gggtgtatcg ttttgccatt gtccgcccat aaacggctgt aacagagtgc gcagctcacc
    15121 tggggccagt tgcgccagct ccagcacatc ttgctgcttt ccggtttcca gtgccttttc
    15181 catcatcgca ttgcgtagcg attctggcgt gaccagaaag cgtaattgtc cgcgctttcc
    15241 gcgtcctgac tgcgcctgcc actccagcca tcccgcttcc tgtgcctgac gcaacagcgt
    15301 acgaacatgg cgttcgctgc aaaaacagcg ttcggccagt tcgctgacgg tgacggtttg
    15361 cggctttccg gcggaaggtt gccacagacg ttgatactgg ttaaggcggt tgagcagtcg
    15421 cataaaaacc cggaacaata ttatttaact attcactatt acttccgtat atatcaggtg
    15481 atactcaatc accattaacc gtgtcacaga gtggagaaag aaatggctcg tctggcagca
    15541 tttgatatgg atggcacttt attgatgcct gaccatcatt taggtgagaa aaccctctct
    15601 actttggcgc gactgcgtga acgcgacatt accctcactt ttgccacggg gcgtcatgcg
    15661 ctggagatgc agcatattct cggggcgata tcgctggatg cgtatttgat taccggcaac
    15721 ggaacgcgcg tgcattctct ggaaggtgaa cttttacatc gtgatgattt acctgcggat
    15781 gtcgcggagc tggtgctgta tcagcaatgg gatacccgag ccagcatgca tattttcaat
    15841 gacgacggtt ggtttaccgg gaaagagatc cctgcgttgt tgcaggcatt tgtctatagc
    15901 ggttttcgtt atcaaataat cgatgtcaaa aaaatgccac tcggcagcgt caccaagatc
    15961 tgcttctgtg gcgatcacga cgatcttaca cgtttgcaga tccagctaca cgaagcatta
    16021 ggcgagcgtg cacatttgtg tttttccgcc acggattgcc ttgaagtgct gccggtgggc
    16081 tgcaataaag gcgctgcact gacggtgctg acccaacatt taggtttatc gttgcgcgat
    16141 tgcatggcct ttggtgatgc gatgaacgat cgcgaaatgt taggcagcgt cggtagcgga
    16201 tttattatgg gcaatgcgat gccgcaactg cgcgcggagc tcccgcattt accggtgatt
    16261 ggacattgcc gaaatcaggc tgtctctcac tatttgacgc actggctgga ctatccacat
    16321 ctaccttatt cccccgaata acgagatccc ttccagcacc gggcaattgc ccggtttttt
    16381 tgcgttgaat ttgtcatttt gtgccgtggt gtttaaaccg cacagaataa attgtcgcga
    16441 tttcaccttt aaaatagaat taaaagagaa aaaaattctc cgtggaaggg ctatgttaga
    16501 taaaattgac cgtaagctgc tggccttact acagcaggat tgcaccctct ctttgcaggc
    16561 actggctgaa gccgttaatc tgacaaccac cccctgctgg aagcgcctga aacggctgga
    16621 ggacgacggt atccttatcg gcaaagtcgc cctgctggat ccggaaaaaa taggcctcgg
    16681 cctgaccgcc tttgtgctga taaaaacgca acatcacagc agcgaatggt attgccgctt
    16741 tgtcacggtg gttaccgaaa tgccagaagt gctggggttc tggcgcatgg ctggtgaata
    16801 cgattatctg atgcgcgtcc aggttgccga catgaaacgc tacgacgagt tttataagcg
    16861 tctggtaaac agcgtgccgg ggctgtcgga cgtcacttcc agcttcgcga tggaacagat
    16921 taaatacacc acttctttac ccatcgaata aatatccaga atcaggtcag gacacaacgc
    16981 gtgcgattat ttgctcaatt aagctggtat ttccgtcggg aatggcgtcg ctatctcggg
    17041 gctgtcgcct tgcttgtcat tatcgcgatg ctgcaactgg ttccaccaaa agtggttggt
    17101 attgttgtcg atggcgtgac agaacaacac tttactaccg ggcagatcct gatgtggatc
    17161 gccaccatgg tgctgattgc cgttgtggtt tatctcctgc gttacgtctg gcgcgtattg
    17221 ctgtttggtg cgtcttatca actggctgtt gaactgcgtg aagattatta ccgtcagcta
    17281 agccggcagc atcctgagtt ttacctgcgt catcgcacgg gtgacctcat ggctcgtgcg
    17341 actaatgacg tcgatcgcgt cgtgtttgcc gccggagaag gggtgctgac gctggtggat
    17401 tcactggtaa tgggctgcgc tgtgttgatt atgatgtcta cgcaaattag ctggcagttg
    17461 accttatttg ccctgttacc gatgccagtg atggcgatca tgattaagcg caacggcgat
    17521 gctttgcatg aacgctttaa gctggcacag gcggcgtttt ccagtcttaa tgaccgcacc
    17581 caggaaagcc tcaccagtat ccgcatgatc aaagcctttg gtctggaaga tcgccagtcg
    17641 gcgttatttg ccgcggatgc cgaagatacc ggcaaaaaaa acatgcgggt ggcgcgtatt
    17701 gatgctcgtt tcgacccgac catctatatc gcgattggta tggcgaactt gctggcgatt
    17761 ggcggcggta gttggatggt ggtgcagggc agtttaacgc tgggccagct caccagtttt
    17821 atgatgtatt taggtctgat gatttggcca atgctggcgc tggcatggat gtttaacatt
    17881 gtggaacgtg gtagtgctgc gtacagccgt attcgcgcga tgctggcgga agcgccggtg
    17941 gtgaacgatg gtagcgaacc cgtgccggaa gggcgtggcg aactggatgt aaatattcac
    18001 cagttcacgt atccgcagac tgaccatcct gcgctggaaa acgtcaattt cgccctgaaa
    18061 cccggtcaga tgctgggtat ctgcgggccg actggttccg gcaaaagtac cctgttgtcg
    18121 ctcattcagc gtcatttcga cgtcagcgag ggggatattc gctttcatga tattcctctg
    18181 acgaagttac aactcgatag ctggcgtagc cgcctggcgg tagttagcca gacgccattc
    18241 cttttttctg acaccgtggc gaataacatc gcgctgggtt gcccgaatgc cacccagcaa
    18301 gagattgagc atgtcgcgcg gttagccagc gtacatgacg atattttgcg tctaccgcaa
    18361 ggttacgata cagaggtggg cgagcgcggt gtgatgcttt ccggcgggca aaaacagcgt
    18421 atctccattg ctcgtgcgtt attagtcaac gcggaaatcc tcatccttga tgatgcgctt
    18481 tcggcggtgg acggacgcac tgagcaccag atcctgcata acctgcgtca gtgggggcag
    18541 ggaagaacgg taatcatcag tgcccatcgc ctttctgcac tgacggaagc cagtgaaatt
    18601 attgtgatgc agcacggaca tatcgcccag cgtggcaatc atgatgtgct ggcacaacaa
    18661 agcggctggt atcgcgatat gtatcgctat caacaactgg aggcggcgct cgacgacgct
    18721 ccggaaaatc gcgaggaggc cgtcgatgcg tagttttagc caactgtggc cgactctcaa
    18781 gcgcctgtta gcgtacggtt caccgtggcg taaaccgctg gggattgcgg tcctgatgat
    18841 gtgggttgcg gcggcggcag aagtcagtgg gccgctgctt atcagctatt ttatcgacaa
    18901 tatggtagcg aaaaataacc tgccgttgaa agtggttgca gggctggctg cggcgtatgt
    18961 tgggctgcaa ctgtttgccg ccgggctaca ttacgcgcag tcgctgctgt ttaatcgggc
    19021 ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg gatgctgcgt tacgccagcc
    19081 attaagcgag tttgataccc aacccgtcgg gcaggtgatt tcccgcgtca ctaatgacac
    19141 tgaagtgatc cgcgatctct acgttaccgt agtggcaact gtcctgcgca gtgccgcact
    19201 ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg cgaatggcgc tggtggcgat
    19261 aatgattttc ccggtggtgc tggtggtaat ggtgatatac cagcgttaca gcacgccaat
    19321 tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac ggctttaacg aaatcatcaa
    19381 tggcatgagc gttatccagc agtttcgtca gcaggcgcga tttggcgaac gtatgggtga
    19441 ggccagtcgt tcacactata tggcgaggat gcaaaccctg cgcctcgacg gttttctgct
    19501 gcgtccgctg ctgagtctgt tttcatcgct cattctctgt ggcttgttga tgctgtttgg
    19561 cttctccgcc agcggcacca ttgaagtggg cgtgttgtat gcgtttatca gctatcttgg
    19621 gcgacttaac gaacccttaa tcgaactgac cacgcagcag gcgatgctgc aacaggctgt
    19681 tgttgctggt gagcgcgtgt ttgagctgat ggacggaccg cgccagcaat atggcaatga
    19741 tgatcgcccg ttacagagtg gcaccatcga agtcgataac gtgtcatttg cttatcgcga
    19801 tgacaatctg gtgctaaaga acattaatct ctctgtgcct tcgcgtaact ttgtggcgct
    19861 ggtcgggcat accggcagtg gtaaaagtac cctcgccagt ttattgatgg gctattaccc
    19921 gctaacggaa ggtgagattc gccttgatgg tcgtccatta agttcgctaa gtcacagcgc
    19981 gctgcgccag ggcgtggcaa tggtgcagca agatccggtg gtgctggcgg ataccttcct
    20041 cgccaacgtg acgctggggc gggatatctc cgaagaacgc gtctggcagg cgctggaaac
    20101 cgtgcaactg gcggagctgg cacgcagtat gagcgacggg atttatacgc cgctgggcga
    20161 gcaggggaat aatctctctg tcgggcaaaa gcaactgctg gcactggcgc gcgtgctggt
    20221 cgagacgccg caaatcctga tccttgatga ggcaaccgcc agcattgact ccggtactga
    20281 acaggcgatt caacatgctc tggcggcggt gcgtgaacat accacgctgg tagtgattgc
    20341 tcaccgctta tcgactattg ttgatgccga cactattctg gtgcttcatc gtgggcaagc
    20401 cgtggagcag ggcactcacc agcaactgct ggcggcccag ggacgctact ggcagatgta
    20461 tcaactgcaa cttgcgggcg aagagcttgc agccagcgtg cgtgaagaag aatcattgag
    20521 cgcctgaata gcgcaatatc tcatcgttgg tgcaaaaatg taacgcactg tgcactgtca
    20581 tagtgcgttt tcattttcaa tcttcttaac ttcctgctct ctttctcgtt tttcatttct
    20641 ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca ttaccgaatt ctgaccggag
    20701 gggatctatg aagctggtga ccgtgataat caaaccattc aagctggaag acgttcgtga
    20761 agcgttatct tccattggta ttcagggcct gaccgtcacc gaagtgaaag gtttcgggcg
    20821 tcagaaaggg catgccgagc tgtaccgggg ggcggaatac agcgtcaact tcctgccaaa
    20881 agtaaaaatt gatgtggcga ttgctgatga ccaactcgat gaagtgatcg atatcgtcag
    20941 taaggcggct tacaccggaa aaattggcga cggcaaaatc ttcgtcgctg aattgcaacg
    21001 cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg ctgtaatctc tggcacacag
    21061 caacaggaac gaaaaatgaa gatagcgacg ataaaaactg ggcttgcttc actggcgatg
    21121 ctgccgggac tggtaatggc tgcacctgcg gtggccgata aagccgacaa tgcgtttatg
    21181 atgatttgta ctgcactggt gctgtttatg actattccgg ggattgccct gttttacggt
    21241 gggttgattc gcggcaaaaa cgtgctgtcg atgctgacgc aggtgacggt gacgtttgca
    21301 ctggtctgta ttctctgggt ggtttacggt tactcgctgg cgtttggtga gggcaacaac
    21361 ttcttcggca acattaactg gttgatgctg aaaaacatcg aactgacggc ggtgatgggc
    21421 agcatttatc agtatatcca cgtagcgttt cagggatcgt ttgcctgcat taccgtaggc
    21481 ttgatagttg gggcgctggc ggaacgaatc cgcttctcag ctgtgttgat tttcgtggtg
    21541 gtatggctga cgctctctta cattcctatt gcgcatatgg tgtggggcgg tggtttgctg
    21601 gcttctcacg gtgcgctgga tttcgcgggt ggcacggtgg tgcatattaa cgccgcaatc
    21661 gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg gcttcggtaa agaggcgttt
    21721 aaaccgcaca acctgccgat ggtcttcacc gggactgcca ttctctatat cggttggttt
    21781 ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg cggcactggc atttgtgaat
    21841 actgtggtcg caacggcggc ggcaattctt ggctggatct tcggtgaatg ggcgctgcgt
    21901 ggtaagcctt cactgctggg agcgtgttct ggcgcgattg ccggtctggt cggcgtgacg
    21961 ccagcgtgtg gctacattgg tgttggcggc gcgttgatta tcggcgtggt agctggtctg
    22021 gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc gggtggatga tccctgcgat
    22081 gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta tcatgactgg gatttttgcc
    22141 gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga cgatgggcca tcagttgctg
    22201 gtacagttgg aaagcattgc cattacgatt gtgtggtctg gtgtggtggc atttatcggc
    22261 tacaaactgg cggatctgac ggttggtctg cgtgtgccgg aagaacaaga gcgagaaggg
    22321 ctggacgtca acagtcacgg cgagaatgcc tataacgcat aacaagcact gcaaaaaaca
    22381 gccggacggt tttcacctcc ggctattttt ttaattgtga ttacgcatca ccccttcctg
    22441 aacggtcgag gcaaccagta cgccgtcctg ggtataaaac tcaccgcgca caaagccacg
    22501 tgcgctggac gccgaggtgc tctccacgct atacagcagc cattcattca aattaaacgg
    22561 gcgatggaac cacatggaat ggtcaatggt ggcaatctga atccccggtt cgagaaaacc
    22621 gataccgtgc ggctgtagag ctaccggcag gaagttaaga tcagaagcgt aaccgagcag
    22681 atactgatga acgcgcaggt catccggcac gctaccattt gcgcggatcc acacctgacg
    22741 atgtggttct gcgacgtgac ctttcagtgg gttatgaaac tccaccggac ggacttccag
    22801 cggacgatcg cagatgaatt tatctttaag cactggcggc agcaggtgcg ccagcgattg
    22861 ggcgatttgc gtttccgaag gcaggccatc aggcgctggc gcggacggca ttgttttttg
    22921 atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc atataaaaaa tcggtttgcc
    22981 gttttgaata gcagcaaccc ggcgggcgct gaagctgtta ccgtcacgca gcgtttcgac
    23041 atcataaata atcggcttct tactatcgcc agggcgaaga aagtagctgt gaaacgaatg
    23101 taccagccgc tcttcaggga cggtctcttt tgcagcatac aaggcctgac ccacgacctg
    23161 gccgccaaat acctggcgta aacctaaatc ttcactctgg ccgcgaaaga gtccttcctc
    23221 aattttttcc agatttaaca atgtcagtaa attttttagc gcctgactca tataactctc
    23281 cagtaacaaa gctgccgcag caagccaaag tgagttgagt ataacgcaaa tttgctactg
    23341 gtccgatggg tgcaatggtc tgaattacgg gctaattaca ggcagaaatg cgtgatgtgt
    23401 gccacaattg ttgacgttac tattttgtta accactcttc cggcgaggaa agttagcccg
    23461 ctggtgcatt gataataagg agaaatgaat gaaactcgtg cacatggcca gtggtttagc
    23521 ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg gatattcaga cgccagcccc
    23581 ggctgcaaat acgtctattt cagcaacaca acaacctgct atccagcaac cgaatgtctc
    23641 cggtaccgtc tggatccgtc agaaagtcgc actgccgcct gatgctgtgc tgaccgtgac
    23701 actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg ctggcgcaga aagcggtgcg
    23761 tactgaaggt aaacagtcac cattcagctt tgttctgcca tttaacccgg cagatgttca
    23821 gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg aatgacaaac tggtatttat
    23881 taccgatacc gttcagccgg tgatcaacca ggggggaacc aaagccgacc tgacattggt
    23941 tccggttcag caaaccgccg tgcctgtcca ggccagtggt ggcgcaacaa ctaccgtacc
    24001 atcgacttca ccgacccagg tgaatccatc ttcggcagtt cctgccccta cgcaatacta
    24061 agcgttacta acccctttcc atctggagag gggttagtag ttccagcgat agcgctgtaa
    24121 gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct gtcgttgacg
    24181 ctgtaaatcc ggtccggtta gcgaaatggt gccgtggcga ttaaccaccc ggtgccaggg
    24241 caaggtgctg ccttcgggga gacgctttaa cacaccgcca acctggcgcg cggcgcgggg
    24301 cgatcccgcc agtttcgcca catcaccgta agtggtgaca tacccttcgg gaatagcggc
    24361 tacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt cttcctgaga
    24421 taatttttca gcataatctg gaaaaacgcc cgagtgaagc cgcattgcgc aagaaaccag
    24481 catctggcac gcaatgggtt gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt
    24541 ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac tctgtttacc
    24601 aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga tgtagctggc
    24661 agggccccca cccatttctg cctcccaccg tttcgtcaaa aaatcccaac atgactaaac
    24721 tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc ggaggtatgt
    24781 ctgatgaagt atgttgatgg ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat
    24841 cgggaaatgg ccgctaaggc cgcgccattg tttaaagagt ttggcgcgct tcgtattgtc
    24901 gaatgctggg ccagcgatgt accggatggc aaagtgaccg attttcgtat ggcggtgaaa
    24961 gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa agaggtccgc
    25021 gacgcagcta atcaaaagat gatgtcggac ccacggatga aagagttcgg cgagtccatg
    25081 ccgtttgacg gcaagcgaat gatctatggc ggattcgagt caatcatcga cgaataggta
    25141 gcgtgacggg ctgtgcggcg tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag
    25201 cgtaagaaat cttctttcgg taatgccttg ctgtagagcc agccctgacc gtagtgcacg
    25261 ccatgctggc gtagccactc ttcttgttta ctggtttcga ttccctccgc taccattttc
    25321 agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata ttccagcgca
    25381 tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa ataacttaaa
    25441 cttgaatacc ccgtaccaaa atcatcaaga taaatttcat ggcccgcctc ccggtagcga
    25501 gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg
    25561 atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg cgggattttt
    25621 tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgtgg atgctgacgc
    25681 agccagtcgc ccatatcttc aaagacgctt cttataatca gtagcgtcaa tggctcagaa
    25741 aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact accgtctgtc
    25801 tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa ggagacaatc
    25861 ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg atgatgcggt
    25921 gactggatgc ggcgcaaaat gcgcagcaca aacatcgctg ccagcaggcc aatcaccaac
    25981 ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat tttcgatgaa
    26041 gcccaggtga taattgaaat attcatctcc gggaagggca ggatatcgta aatgattcca
    26101 ttattttcga tatgctcacc gggtgttttt tgtagcctgg taataattcc ctgagcaatt
    26161 tcatcgctgc tggttatgac aacgttatgg gcattgccaa taatggcggc atcaatttgc
    26221 catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac gacataatgt
    26281 gccgttccca tggcgaccat gtaacggata atgcctaaat cgttatgtga tgttaaccag
    26341 acacgataac catctttcga aattttacct ggctcgggga aggtatcggg cggactttca
    26401 tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc ctgaatatag
    26461 cgataactgt aagatacccg acgcatttcc atgagatggg cttcactaca gtcagcgcct
    26521 tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt attggcccga
    26581 atagcgacgc gggaggaata ggtatccagc tcttcaataa acgatgtttc tacctgctga
    26641 tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag tactcccgaa
    26701 ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca gtgtagggaa
    26761 agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagct ggtatttcat
    26821 gaagtgatgg gtggaaacga tcgttgtcat ttgaccaggc gcagagcttt acgcctggta
    26881 tgtaatccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga
    26941 caattcacct ttacggacga tcattttttg cagttgttcc ttatgaacat ccgcgacacg
    27001 cggagagttc aagatggctt caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc
    27061 gccgttatag caaacgcgga aatccaacac gtcgatatct tcatagcggt agcgtgggta
    27121 gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta gccagacaga
    27181 gcctgaaagc gtttcggact gcaacatcac cgccagggtg gcaaaaaaag caacgtacat
    27241 accgataaac agccccggat gtttacggat aaaactgata ctaaagcggg gtttgttgtc
    27301 gcgtttttcg cgggtattca gagactcaat cgtttcggtc agcaggcgtt gtatttcggt
    27361 catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt aacgtgggaa
    27421 tagtagaggg aaagtaacag atctgccaca attttgcata acagttgcga aaaaccgatt
    27481 acaatttttt aattattctg gctggattac cgcccacgac aacgttgtcc gggacatctt
    27541 ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca ccagggttaa
    27601 tgaccgcgcg tccgccaatc cagacgttat taccgatggt gacgggtttc cccagttcag
    27661 caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata tgaacgccta
    27721 gtgccaacat acagttatca ccgatgcgaa tagggcagac atcaagcatc acgcaatcga
    27781 agttggcgaa aaaattatta ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg
    27841 gctcaatata agcctctgtc acctgaccga atagatcagc gagaatttgc tggcgtaatg
    27901 tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat
    27961 cgcgagataa cgtctcatct gccgagcgat acaactcacc agcaatcatc ttttcttttt
    28021 ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag acgaaaagtc
    28081 agtgaagaga acgttcccat tacatctgtg tgataaagat cacatagggt atgtcttcat
    28141 ggcgaaaaag tataaaattc ttaataaaca gccggttata gctccgaaag cgaattattt
    28201 attagcgaat aaatttccat actgaggaag ggatcttgtc gtacagttta ttcatggtca
    28261 attcggcgag gcggtgatct gcggctgagt aaaataccgc cagttcatta tctgataatt
    28321 cgtatttatt tttctcgata acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac
    28381 gcattaaata atcggttttc gttaaaggtt tttcggacat acttctaccc atggttgtaa
    28441 taattctaac aagataaact cgcaggattc tctttcgtcg cattgacaaa acaacggaat
    28501 agtcgattac ctgacttccg ccatttctga agatcctgca tattaatacc ataactactg
    28561 aacaacataa aggtgtcatc cagatattcg tcgatctgct caatgagctt attgtcttca
    28621 ttatacttaa ttttgtaatt aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac
    28681 tggaggttga tcgccgaggt tgggtcgtta acccagccat gattgctttc ttcaaggttt
    28741 gcaaggcagt catgatacag agtttcacag agaaacttaa gctgtgcgat atcatgtctt
    28801 ttgggtgagt attcatccat aacgcgtccc cttcttagcg gttgaactaa cggacacctt
    28861 tcgggatgga aaaaacttac tgacctggac ttgccctctc gtttgttagc ttaactataa
    28921 gccactcttt gcaggttttc atcgcattat tacgaaaaat tacaattagg aaggaagcgg
    28981 gacagggctc actttttcat actagcacca acccgcagca ggtaaaagca gtttgctcga
    29041 actgttcata acatttcagg attatcgcca gatgaaattg gtgacactaa taccaggatt
    29101 gctctgaata tgacgtaata accgaggaat gaataaagaa ttaccgcaaa taattaagaa
    29161 tagcctctaa atgattatgg attattaaat ctattagcgc tacttaatat aaattaacta
    29221 acaatcagta ttttttatga attttatacg tggttaatac tggttttcgt atgagatcct
    29281 gagttggtgg ttcaattact ccttaatgtt cgtaggttat gcataaaaaa ggccgcttac
    29341 gcggccttag tgattacacg ttgtatcaat gatgatcgac agtatggttg tgctcgatat
    29401 cttcattctt gcggctaaag cggcggcgaa ccaccacaaa gaataccgga acgaagaaga
    29461 ttgccagtac cgttgcggtc accatcccgc ccattacacc ggtacctact gcgttctgcg
    29521 cgccggaacc agcaccagta ctgataacca gcggcataac gccgaggata aacgccagcg
    29581 aggtcatcag gatcggacgt aaacgcatcc gcaccgcatc aagcgtcgct tcaatcagac
    29641 ctttaccttc tttatccatc aagtctttgg cgaattcgac gataagtatc gcgttcttcg
    29701 ccgacaaccc aatggttgtg agcaggccta cctggaagta aacgtcattg gtcaggccac
    29761 ggaaggtggc agccagcaac gcaccgataa cccccagcgg aacgaccagc ataacggaga
    29821 acggaatcga ccagctctcg tacagcgccg ccagacacag gaacacgaca atcaacgaaa
    29881 tcgcgtacag tgaaggtgcc tggttgccgg agagacgttc ctgataggac atccccgtcc
    29941 agtcatagcc aacaccggta ggcagtttgc tcgccagttg ttccatcagc tccattgctt
    30001 caccggtact tttacccggt gccgcctggc ctaagatttc catggatggc aggccgttgt
    30061 aacgttccag acgcggcgaa ccgtactccc aacgagaaga ggagaacgcc gagaatggca
    30121 ccatctgacc atcagcagca cgaacatacc agtcgccgat atcatccggc agcatacggt
    30181 atttcgcttc tgacatgacg taaactttct tcacacgacc gcggtcgata aagtcgttca
    30241 catagctgcc gccccatgca gcgcccagag tggtgttaat gtcgttgata gaaacaccca
    30301 gcgcctgcgc tttttcctgg tcgatatcaa tcttaaactg cggggtatct tccagaccgt
    30361 ttggacgtac gctggtcaac atatcagggt gcttcgctgc ttctgcaagc aactggttac
    30421 gcgcctgagt cagtttttcg tgaccaaggc cagcctggtc aatcagctca aagtcaaagc
    30481 cggttgcggt acccagttcc acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt
    30541 tgatttgcga gaaagcgcgt gttgcacgca tggtaatcgc ttcaactttg ttttcttcac
    30601 ccggacgatc ggcccagtcc ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc
    30661 ccgcaaagcc gaagccgtta acggcgaaca ccgactcaac gttgttcttt tctttggtca
    30721 gatagtaatg cgttacctca ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg
    30781 gcagctgaac catggtcata aatacgccct ggtcctcatc tggcaagaag gagcttggca
    30841 gacgcacaaa cagataggcc atgccgacca cgatgatcag atacagcacc agataacgcc
    30901 ccgtactgcg cagaataccg cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca
    30961 tgcggttaaa ccagccgaag aagccttttt taccttcccc gtgatcgcct ttggcaatcg
    31021 gtttcagcat ggtggcacaa agagctggag tcaggatcaa cgccaccagt accgacagcg
    31081 ccattgctga aacaatggta atagagaact gacgataaat tgccccagta gaaccgccaa
    31141 agaaggccat cggtacgaat accgccgaca gtaccatcgc gataccgacc agagcgccct
    31201 gaatctgccc catcgactta cgggtggctt cttttggcgg caaaccttct tccgccatca
    31261 cacgctcaac gttttctacc acaacgatag cgtcatccac caacaggccg atggcgagca
    31321 ccatcccgaa cattgttagc gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg
    31381 tcccgagcaa taccaccggt acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca
    31441 ggaacagata cataaccagg aacacgagga tgatcgcttc gaccagcgtt ttaaccactt
    31501 cgtgaataga gattttcacg aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacccg
    31561 acgggaagaa cggttccatc ttcgccagtt cagcacggat tgccgcagcg gtatccagcg
    31621 cgtttgcacc ggtcgccagc ttgatcccca gaccggaagc cggttggccg ttaaactctg
    31681 cgatgatgtc gtagttctca ccacccagct caatcttcgc cacatcacgc agcagtacgc
    31741 gggaaccatc ctgattcact ttcagcagga ttttgccgaa ctcttcagta gaggtcagac
    31801 gcgtctgagc aataatagag gcgttaagct gttggccttt caccggcggt gtaccaccga
    31861 gctggcccgc cgcaacctgg gcgttctgcg ctttgatggc ggtaataaca tcaaccggcg
    31921 ttagctggaa tttgttcagt tcattcgggt tcatccagat acgcatcgcg tactgtgaac
    31981 cgaacaactg aacgtcaccc acgccagacg tacggctgat ggcatctttc atattcgccg
    32041 ccacgtagtc ggagatatcc tcctgcgtca tggtgccatc ggtgttgata acgccgacaa
    32101 ccatcaggaa gctgctggat gatttctcaa tgctcacccc ttgctgctga acttcttgcg
    32161 gcagcaacgg catcgccagc tgcagtttgt tctgcacctg aacctgcgcg atatccgcat
    32221 cagtaccaga ctcaaaggtc agggtgatct gtacggtacc cgtggagtca ctgttagagg
    32281 acatgtacat caggttatcg ataccgttca tattctgttc gataacctgt gtcaccgtgt
    32341 cctgcactgt tttcgcatca gcgccggggt aggaggcgga gatcgttact gccggcggtg
    32401 caatcgtagg atattgcgcc accggcagtt tgaggatcgc cagcccccct gccaacatga
    32461 tgataatggc gatcacccac gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt
    32521 aacggctcct gtttaagtta agacttggac tgttcaggct gagcaccgct tgcggcttgc
    32581 tggttattat cagcggtaac ttcttgtgct tttacctgga caccaggacg cactttctgc
    32641 agcccactta ttactacgcg atcgcctgct ttcagacctt ctgtcaccag ccacttatcg
    32701 ccaatagcct ggcttgcaac gatcggacgg gtttccactt tgtcatccgc gccaaccacc
    32761 agtacggtgg catcgccacg cggcgtacgg gttacgccct gttgcgggac taaaatagcg
    32821 tttggattaa gcccttcttc cagacgtgcg cgcacgaaca tacccggcag cagagtgtga
    32881 tccgggttcg ggaagatagc gcgtagggtg atagacccag tggtctgatc aacggtaacg
    32941 tcagagaatt ccagcgtacc gtcctgcggg aacttaatgc cgtcactggt gatcagcgac
    33001 actttggctt tgccgttctc ttgtttcagc gtgccattcg ccagttcctg tttcaggcgc
    33061 aggaagtcgt tgctggactg ggtcacatca acgtagatcg gatcaagttg ctgcacggtt
    33121 gccagcgcag tcgcctgacc gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca
    33181 atgcgaccgc taatcggaga ggtgactttg gtgtaagcca gattgatccg cgcagtttca
    33241 acggcagctt tcgccgcagt taccgcagca ttcgcctgtt gcgcatcagc cagagcctga
    33301 tcgtactctt gcttactgat gtactgagta ccgagcagtt tctgataacg attcaccgtc
    33361 aattgcgcga tattggctgc agcctgggct ttcgccagat cacctttcgc actgtcgtat
    33421 gtcgcctgat aggtcgcagg atcaatctga tagagagaga cacctgcttc gatgtcgcta
    33481 ccttctttga aattacgctt caggataatc ccgctaactt gaggacgaac ttctgcgatc
    33541 cggtaggcac tggtgcgacc cggaagctcg gttgtgatct gcagaggttc agttttgact
    33601 gttactacgc caacggcggg catctgctgg ccaccttgtt gggcctgttt gtcgtcacat
    33661 cctgttaggg ctaagctgcc tgagagcatc agaacgaccg ccagaggcgt aaaccctctg
    33721 tttttgttca tatgtaaacc tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta
    33781 ataaacccat tgctgcgttt atattatcgt cgtgctatgg tacatacatt cacaaatgta
    33841 tgtaaatcta acgcctgtaa attcacgaac atatggcacg aaaaaccaaa caagaagcgc
    33901 aagaaacgcg ccaacacatc ctcgatgtgg ctctacgtct tttctcacag cagggggtat
    33961 catccacctc gctgggtgag attgcaaaag cagctggcgt tacgcgcggt gcaatctact
    34021 ggcattttaa agacaagtcg gatttgttca gtgagatctg ggaactatca gaatccaata
    34081 ttggtgaact agagcttgag tatcaggcaa aattccctgg cgatccactc tcagtattaa
    34141 gagagatatt aattcatgtt cttgaatcca cggtgacaga agaacggcgt cgattattga
    34201 tggagattat attccacaaa tgcgaatttg tcggagaaat ggctgttgtg caacaggcac
    34261 aacgtaatct ctgtttggaa agttatgacc gtatagaaca aacgttaaaa cattgtattg
    34321 aagcgaaaat gttgcctgcg gatttaatga cgcgtcgggc agcaattatt atgcgcggct
    34381 atatttccgg cctgatggaa aactggctct ttgccccgca atcttttgat cttaaaaaag
    34441 aagcccgcga ttacgtcgcc atcttactgg agatgtatct cctgtgcccc acgcttcgta
    34501 atcctgccac taacgaataa ccctgaatct gactccagga tttttcctgg acattttcgt
    34561 cgttgctatt ctggttcact gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt
    34621 cttcaggttc agaaaccttc attcatcatg actatgttcc agtattacaa acgatcacgg
    34681 cattttgttt tttcagcatt tattgctttt gtttttgtct tgttatgcca gaacacggcg
    34741 tttgcgcggg cgtcatcgaa tggtgatctg ccgacaaaag cggacctgca ggcgcaactt
    34801 gactcactaa ataaacaaaa agatctttct gctcaggaca aactggtgca gcaggatctg
    34861 acagatacat tagccaccct cgataaaatc gatcgcgtaa aagaagagac cgttcagcta
    34921 cggcaaaaag tcgctgaagc gccggaaaaa atgcgccagg cgaccgcggc gttaacagca
    34981 cttagcgatg tcgataacga cgaagaaacg cgcaaaattc tgagcacgct ttcgttgcgc
    35041 cagctggaaa ctcgcgttgc ccaggcgctg gacgatttgc aaaacgcaca aaacgatctg
    35101 gcgtcttata acagccagct ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg
    35161 atgtataacg cttcgcagca gctgcaacaa attcgcagtc gtctggatgg gactgatgtc
    35221 ggcgagacgg ccttacgtcc cagccagaaa gtgttaatgc aggctcagca ggcgttgctg
    35281 aatgcggaga ttgaccagca gcgtaaaagc ctggaaggga ataccgtctt gcaggatacc
    35341 ttgcaaaagc aacgtgatta cgtgacggcg aacagcgccc gtctggagca ccagttgcag
    35401 ctgttgcaag aagcggtaaa cagcaagcgc ctgactttaa ccgaaaaaac ggcgcaggaa
    35461 gccgtctccc cggatgaagc cgcgcgtatt caggctaatc cgctggtgaa gcaggaactg
    35521 gaaattaacc agcagttaag tcagcgtctg attaccgcga ctgaaaacgg taatcagttg
    35581 atgcagcaaa acattaaagt caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat
    35641 attaaagagc agattgccgt cctgaagggc agcctgctgt tgtctcgtat cctttaccag
    35701 caacaacaaa cgctgccctc ggcggatgaa ctggaaaaca tgaccaaccg catcgcggat
    35761 ttgcgtctcg aacagtttga agttaaccag cagcgtgatg cactcttcca gagcgatgcg
    35821 ttcgtcaaca aactggaaga aggtcacacc aacgaagtca acagcgaagt tcacgatgcg
    35881 ttattgcaag tggttgatat gcgtcgcgaa ttgctggatc aactcaacaa acagttgggt
    35941 aaccagctga tgatggccat taacctgcaa atcaaccagc agcagttaat gagtgtgtcg
    36001 aaaaacctga aatccatcct gactcagcaa atcttttggg tgaacagtaa ccgtccaatg
    36061 gactgggact ggatcaaagc gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa
    36121 attacggtta actgggaaaa agcctggccc gccgttttta tcgctttcct cgctggtttg
    36181 ccgctgctgt tgattgccgg gctgatccac tggcgtctgg ggtggctgaa agcgtatcaa
    36241 caaaaactgg cttccgctgt gggttccctg cgtaacgaca gccagctcaa cacaccaaaa
    36301 gcgatcctta tcgacctgat ccgtgcactg ccggtgtgcc tgattattct cgcggttggc
    36361 ctgattctgt tgaccatgca gctcaacatc agcgaactgc tatggtcgtt cagcaaaaaa
    36421 ctggcgattt tctggctggt gtttggcctg tgctggaaag tgctggagaa gaacggcgtt
    36481 gccgtgcgtc acttcggcat gccggaacag cagaccagcc actggcgtcg gcaaattgtc
    36541 cgcatcagtc tcgcgttgct gcctatccat ttctggtctg tggtggcaga actttccccg
    36601 ctgcatctga tggatgatgt gctggggcag gcgatgattt tcttcaacct gctgctgatt
    36661 gccttcctgg tatggccaat gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg
    36721 cgactggtca ccattaccgt gctgtcgatt atcccgattg cattgatggt gctgactgcc
    36781 acgggttatt tctacaccac gctgcgcctc tccgggcgtt ggattgaaac cgtttatctg
    36841 gtgatcatct ggaacttgct ctaccagacg gtattgcgcg gcttaagcgt agcggcgcgg
    36901 cgtatcgcct ggcgtcgtgc gctggcgcgt cggcagaatc tggtgaaaga gggcgcagaa
    36961 ggtgccgaac cgccggaaga acccaccatt gcactggaac aggttaacca gcagacgctg
    37021 cgtattacca tgttgctgat gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc
    37081 gatttgatca ccgtgttcag ctatctcgac agcattacgc tgtggcatta caacggtact
    37141 gaagctggtg ctgcggtggt gaaaaacgtc accatgggta gtctgttgtt tgcgattatc
    37201 gcctcaatgg tggcctgggc gttgattcgc aacctgcctg gtttgctgga agtgctggtg
    37261 ctctcgcgac tgaatatgcg ccagggcgcg tcgtatgcca ttactaccat ccttaactac
    37321 atcattattg ctgttggtgc gatgacggtg ttcggatcgc tgggcgtctc ttgggataaa
    37381 ctccagtggc tggccgcagc attatccgta ggtcttggtt ttggtttaca agaaattttc
    37441 ggtaacttcg tctccggttt gatcattctg ttcgaacgtc cggtgcgtat tggcgatacg
    37501 gtaaccattg gtagcttctc ggggacggta agtaaaatcc gtattcgtgc gacaacgatt
    37561 accgatttcg atcgcaaaga agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg
    37621 atcaactggt cgttgactga cactactacg cgtctggtga tccgtctcgg cgtggcctat
    37681 ggctccgatc tggaaaaagt gcgtaaagtg ttactgaagg cggcgactga gcacccaagg
    37741 gtgatgcacg aaccaatgcc ggaagtcttc tttacggcat ttggtgccag cacgttggat
    37801 catgagctgc gtctgtatgt gcgtgaactg cgtgaccgta gtcgtactgt cgatgagctg
    37861 aaccgtacta tcgatcagct gtgccgtgaa aacgacatca acattgcctt taaccagctt
    37921 gaagtgcatc tgcacaacga gaagggcgat gaggtgacgg aagtaaaacg cgactacaaa
    37981 ggcgacgatc cgacgccagc ggtagggtaa ggacaataaa gggcgacagt gatgccgccc
    38041 tttatttttt actcgctttg tgaagcctca tcgctctgta acttcttttc ataatcacgc
    38101 tttacaatat ccagcgcgcg cagcacggtg cgggctggga tctgattttc ttccagcagc
    38161 acaatcaaat cgacagccag tttgacatcg tcaggggcat tttccagtga catactctct
    38221 ccattgctag cgggttaaac gcgctaacct gttttcgatt ttttccagcg catggcggca
    38281 gcgtgccagg cgcgcttcat aggcttccac ttcacgatgc agcgtttgct gttccacgag
    38341 atcggtcacc cgcgccaggc gggctctacg ttcggcaacc atctcacgta gccgccgctc
    38401 aaaatcctga tgctgaatac gtttacgctg ccagcgggca atttggggtg gtgcgctatc
    38461 ccactcgcgt agcgaccagg ctgtggcttc acgcgctata gcctccagtt gcgccgccag
    38521 atgttctgcc agccaggcga cttgcggcag ttgctgttgc tcaactgcat gacgaagcgc
    38581 agccagatta tcgcccgcct tgtcgagaca agcctgtagt gttgtcgcac gagtctgaaa
    38641 aagatgcctg ttgaaacgag cacttagcgt ggcgaactgt gacaccgggg cacaacgctg
    38701 acgcagcgta gcgagctgtc cttccagttt ttccagcagc agggcggttt tcacgataag
    38761 acctcaatga aaatgataat tgttatgcta aagtagccac tctgtaagct gacaacactc
    38821 aatatgcaac gaatcatttt aatcatcatt ggctggctgg cggtagtgct gggcacgctg
    38881 ggcgtggtat taccggtatt accgacgacg ccgtttatcc tgctggcggc ctggtgcttt
    38941 gcccgttctt ccccgcgctt tcacgcctgg ttgctgtacc gctcatggtt tggcagctat
    39001 ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg
    39061 cttattttgc tcacgtttgc catttctctg tggttcgtcc agatgccatg ggtgcgcatc
    39121 atgttgctgg taattctcgc ctgtttgctt ttctatatgt ggcgaattcc ggtgattgat
    39181 gaaaagcaag aaaagcactg aagcacaaca atcgcagttg caattattgc gtacagccag
    39241 tacattctgg cgttttcgag cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt
    39301 tgatacgttt aaaacgcgcc gtgagtacca ccgtaacaag caggcataca cttatgaccg
    39361 cgactgcaca gcagcttgag tatctcaaaa atagcatcaa aagcattcag gactacccaa
    39421 aacccggcat tcttttccgc gatgtcacca gcttactgga agacccgaaa gcttacgctc
    39481 tcagcatcga cttgctggtt gagcgttaca aaaatgcggg cattaacaaa gttgtcggca
    39541 ccgaagcgcg tggcttcttg tttggcgctc cggtagctct gggtctgggc gttggctttg
    39601 taccggtccg taaaccgggc aaactgccgc gtgaaaccat cagtgaaact tacgatctgg
    39661 aatacggcac cgatcagctg gagatccacg ttgatgccat caaaccgggc gacaaagttc
    39721 tggtggtgga cgatctgctg gcaaccggcg gcactatcga agcgaccgtt aaactgatcc
    39781 gtcgtctggg tggtgaagtg gctgacgctg cgttcattat caacctgttc gatctcggcg
    39841 gcgaacagcg tctcgaaaaa cagggcatta ccagctacag ccttgtcccg ttcccgggcc
    39901 attaattatc gccagtcttg tgctgcccac gctacggaca gcacaagatg tgcattcagc
    39961 ctcgccgttc tgacggggct gtgttagcat taccccttcg agaatccacc ttccagcgtt
    40021 tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa tggcgcccac aaacctttgc
    40081 tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg aacggcttgt cgttagggcg
    40141 tattcatcat gcttatcttt tttccggcac ccgtggcgtc ggaaaaacct ctatcgcccg
    40201 actgctggcg aaggggctaa actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg
    40261 cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc
    40321 ctcgcgcacc aaagttgaag atacccgcga cctgctggat aacgtccagt acgctccggc
    40381 gcgtggtcgt ttcaaagttt atctcatcga cgaagtgcat atgctgtcgc gccacagctt
    40441 taacgcactg ttaaaaaccc ttgaagagcc gccggagcac gttaagtttc tgctggcgac
    40501 gaccgatcca cagaaattgc cggtgacgat tttgtcgcgc tgtttgcaat ttcatctcaa
    40561 ggcgctggat gtcgagcaaa ttcgtcatca acttgagcac atcctcaatg aagaacatat
    40621 cgctcacgag ccgcgggcgc tgcaattgct ggcgcgcgcc gctgaaggca gcctgcgaga
    40681 tgccttaagt ctgaccgacc aggcgattgc cagcggtgac ggccaggttt caacccaggc
    40741 ggtcagtgcg atgctgggta cgcttgacga cgatcaggcg ctgtcgctgg ttgaagcgat
    40801 ggtcgaggcc aacggcgagc gcgtaatggc gctgattaat gaagccgctg cccgtggtat
    40861 cgagtgggaa gcgttgctgg tggaaatgct cggcctgttg catcgtattg cgatggtaca
    40921 actttcgcct gctgcacttg gcaacgacat ggccgccatc gagctgcgga tgcgtgaact
    40981 ggcgcgcacc ataccgccga cggatattca gctttactat cagacgctgt tgattggtcg
    41041 caaagaatta ccgtatgcgc cggaccgccg catgggcgtt gagatgacgc tgctgcgcgc
    41101 gctggcgttc catccgcgta tgccgctgcc tgagccagaa gtgccacgcc agtcctttgc
    41161 acccgtcgcg ccaacggcag taatgacgcc aacccaggtg ccgccgcaac cgcaatcagc
    41221 gccgcagcag gcaccgactg taccgctccc ggaaaccacc agccaggtgc tggcggcgcg
    41281 ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa aagagtgaac cggcagccgc
    41341 tacccgcgcg cggccggtga ataacgctgc gctggaaaga ctggcttcgg tcaccgatcg
    41401 cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg ccagctaaaa aagaagcgta
    41461 tcgctggaag gcgaccactc cagtgatgca gcaaaaagaa gtggtcgcca caccgaaggc
    41521 gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg gcggcgaagc tggcggcaga
    41581 agccattgag cgcgacgcgt gggcggctca ggtgagtcaa ctttcgctac caaaactggt
    41641 cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac aacgcagtat gtctgcattt
    41701 gcgctcctct cagcggcatt tgaacaaccg cggtgcacag caaaaactgg ctgaagcgtt
    41761 gagcatgtta aaaggttcaa cggttgaact gactatagtt gaagatgata atcccgcggt
    41821 gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga
    41881 gtccattatt gcggataata atattcagac cctgcgtcgg ttcttcgatg cggagctgga
    41941 tgaagacagt atccgcccca tttgatcgta agcacagctt acgttcgtca tccttaacgt
    42001 gattgagaga gaaacctatg tttggtaaag gcggtctggg taacctgatg aagcaagccc
    42061 agcagatgca agaaaaaatg cagaaaatgc aggaagagat cgcgcagctg gaagttaccg
    42121 gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg tgcacataac tgccgtcgcg
    42181 tagagatcga cccgagcctg ctggaagacg acaaagagat gctggaagac ctggtggctg
    42241 cagcattcaa cgacgcagca cgtcgtattg aagaaacgca gaaagaaaaa atggcttctg
    42301 tatcctccgg aatgcagctg ccgcctggct ttaagatgcc gttctgatgc aaaccagccc
    42361 gctgttaaca cagcttatgg aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc
    42421 gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca
    42481 ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc gattgccgca ccttcaccga
    42541 acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgcgt
    42601 ggtggagagt ccggcggaca tttacgccat tgagcagacg gggcagtttt caggtcgtta
    42661 ttttgtgttg atgggacatc tgtcaccgct ggacggcatc ggtccggatg atatcggcct
    42721 tgatcgtctg gaacagcgtc tggcagagga aaaaatcact gaagtgatcc tcgccaccaa
    42781 ccctacggtt gaaggtgaag ctaccgctaa ctacattgcc gagctttgcg cgcaatatga
    42841 cgtggaagcc agccgaatcg ctcatggcgt accggttggc ggcgaactgg aaatggtcga
    42901 tggcaccacg ctgtcacact cccttgccgg gcgtcataag attcgttttt aagcaaacga
    42961 gagcaggatc acctgctctc gcttgaaatt attctccctt gtccccatct ctccctcatc
    43021 ctgtttttaa ccttaaaatg gcattattga ggtagaccta catgaaagga caagaaactc
    43081 gtggttttca gtcagaagtg aaacagcttc tgcacctgat gatccattct ctctattcca
    43141 ataaagaaat cttcctgcgt gagcttatct ctaacgcctc cgatgcggcg gacaagttgc
    43201 gtttccgtgc gctctctaac ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg
    43261 tctctttcga taaagacaag cgcacgctga ccatctccga taacggcgtg gggatgaccc
    43321 gcgacgaagt gattgaccat ctggggacta tcgctaaatc cggtaccaaa tcattcctcg
    43381 aatccctggg ttctgaccag gcgaaagaca gccagctgat cggtcagttt ggtgttggtt
    43441 tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa
    43501 aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg tgaatatacc gttgccgaca
    43561 tcaccaaaga agatcgtggt actgaaatca ccctgcatct gcgtgaaggc gaagacgagt
    43621 tcctcgatga ctggcgcgtg cgttccatca tcagcaaata ctccgaccat atcgcgctgc
    43681 cggtagagat cgaaaaacgc gaagagaaag acggcgaaac cgttatctcc tgggagaaaa
    43741 ttaacaaagc gcaggcgctg tggactcgta acaagtcgga aatcaccgat gaagagtaca
    43801 aagagttcta caaacacatc gcccacgatt ttaacgatcc gctgacctgg agccacaacc
    43861 gtgttgaagg taagcaggag tacaccagcc tgctgtacat tccgtcccag gctccgtggg
    43921 atatgtggaa ccgcgatcat aaacacggcc tgaaactgta tgttcagcgt gtgttcatca
    43981 tggacgacgc agaacagttc atgccgaact atctgcgctt cgtgcgtggt ctgattgact
    44041 ccagcgatct gccgctgaac gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta
    44101 acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg
    44161 acgcggaaaa ataccagacc ttctggcaac agtttggcct ggtactgaaa gaaggtccgg
    44221 cggaagattt cgctaaccag gaagcgatcg ccaaattgct gcgttttgct tctacccata
    44281 ccgattcttc tgcgcagacc gtgtctctgg aagactacgt ttcccgcatg aaagaagggc
    44341 aggagaaaat ctactacatc actgcagaca gctatgcggc agcgaagagc agcccgcacc
    44401 tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt
    44461 ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt ccagtcggtg tctaaagttg
    44521 acgagtcgct ggaaaaactg gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag
    44581 cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg cgagcgcgtg aaagatgtcc
    44641 gtctgactca ccgtctgacc gatacgccag cgatcgtttc gaccgacgcg gacgaaatga
    44701 gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca
    44761 tcttcgaact gaacccggat cacgtactgg tgaaacgtgc ggcagatact gaagatgaag
    44821 cgaagttcag cgagtgggta gaactgctgc tggatcaggc actgctggca gaacgcggca
    44881 cgctggaaga tccgaacctg tttattcgtc gtatgaacca gctgctggtt tcctgatgta
    44941 atgccggatg accttcgtgt catccggcat ttttcttttc attatctgca ctttccgcaa
    45001 attatctcgc cattaaccgt ttcagcccct ggtgcctttc ttgaggcaat cgcctgatgg
    45061 tggtatcgtt tatcgctttt tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc
    45121 gtatcattct gcttggcgct ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg
    45181 agaaatatgg tattccgcaa atctccactg gcgatatgct gcgtgctgcg gtcaaatctg
    45241 gctccgagct gggtaaacaa gcaaaagaca ttatggatgc tggcaaactg gtcaccgacg
    45301 aactggtgat cgcgctggtt aaagagcgca ttgctcagga agactgccgt aatggtttcc
    45361 tgttggacgg cttcccgcgt accattccgc aggcagacgc gatgaaagaa gcgggcatca
    45421 atgttgatta cgttctggaa ttcgacgtac cggacgaact gatcgttgac cgtatcgtcg
    45481 gtcgccgcgt tcacgcgccg tctggtcgtg tttatcacgt taaattcaat ccgccgaaag
    45541 tagaaggtaa agacgacgtt accggtgaag aactgactac ccgtaaagac gatcaggaag
    45601 aaaccgtacg taaacgtctg gttgaatacc atcagatgac agcaccgctg atcggctact
    45661 actccaaaga agcggaagcg ggtaacacca aatacgcgaa agttgacggc accaagccgg
    45721 ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta atcaagccgc agcagatctg
    45781 ctttcgggca ggtctgctca tccacctcag gcaatacctc tcacagcaat tagttcttct
    45841 tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg taatgcgtca
    45901 gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca cacctgaagc
    45961 ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata cctcacggtt
    46021 gttatggtgg ccattgctgc gcggcgtgat tttgccgctg cgctcgccgc gtgtggcgaa
    46081 gctgtatgcc tctgtctgga tggaaggtgg ctcgccgctg atggtttaca gccgccagca
    46141 acagcaggcg ctggcacaac gtttaccgga gacgcccgta gcgctgggaa tgagctacgg
    46201 ctcgccatca ctggaaagcg ccgtagatga actcctggca gagcatgtag atcatattgt
    46261 ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat gggatgaact
    46321 ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttatac gtgattacgc
    46381 cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt ttgccaaaca
    46441 tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt atgcagatga
    46501 aggcgatgat tacccgcaac gttgccgcac aacgactcgt gaactggctt ccgcattagg
    46561 gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggtcggg aaccctggct
    46621 gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag gtcatattca
    46681 ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga ttgccgagca
    46741 aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc cggcgcttaa
    46801 tgccacaccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc gctaaagctg
    46861 agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca tcatccgtga
    46921 ataatgcaaa aaggcgtgca gcgtgcctgg gtagagtttg aactcacagg gctgctgatg
    46981 cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga actccgcccc
    47041 ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac agtaatacgg
    47101 cgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca aatcctgttg
    47161 cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc gtaatccgta
    47221 aagcccatac cacagcaaaa cgcccgcaac tttaccgcaa tcgatctgtt tatcacgcaa
    47281 ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa agccaatgcg
    47341 ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag cagccacaat
    47401 ttcctctatc acttgcggaa aacgcgcttc aggtgaaagg gtgtaatcaa taccaatcac
    47461 cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat cgagattgcc
    47521 gagaataaaa ccgcctccat gcaaataaaa tagcgttgcc gggctatctg gctgcggaca
    47581 aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctt tggttgccat
    47641 ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc gttgctcagc
    47701 aatcgttccc gttgcgggcc agggcggtaa atccggctga agagtattca caacggtctt
    47761 catttcagca gaaataaggt ccagaacagg taatttgttt tccggcttca tacaaaaact
    47821 cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc cgcctgctga
    47881 tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag
    47941 ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca aacattactt
    48001 ccccgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg aaaccagcgc
    48061 cgcctgggta gtgggtatcg atcaaacgct ggtcgatatt gaagcgaaag tggatgatga
    48121 attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg atgatgtagc
    48181 cgaagcgctt tatcaggaac taaaacagaa aaacctgatt acccatcagt ttgcgggtgg
    48241 caccattggt aacaccatgc ataactactc ggtgctcgcg gacgaccgtt cggtgctgct
    48301 gggcgtcatg tgcagcaata ttgaaattgg cagctatgcc tatcgttacc tgtgtaacac
    48361 ctccagccgt accgatctta actatctaca aggcgtggat ggcccgattg gtcgttgctt
    48421 tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agccctggcc acatgaacca
    48481 gctgcgggct gaaagtattc cggaagatgt gattgccgga gcctcggctc tggttctcac
    48541 ctcttatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca tgaaagccat
    48601 tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggaacta agtttgtcat
    48661 tgccgagaat ccacagtggt ggcagcaatt cctcaaagac cacgtctcta tccttgcgat
    48721 gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg catctgacaa
    48781 ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct tgtatatggc
    48841 gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgttgctgc cgggcgctat
    48901 agccgaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt gtcagaatcc
    48961 gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa tcatgaacac
    49021 caacggagca ggggatggcg cactggcagc gttgctgcat gacattaccg ccaacagcta
    49081 ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa cttattcatc
    49141 gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga accagcattc
    49201 acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt cttactggga
    49261 tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat ctggcatttt
    49321 gctctcaatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt tttcatattt
    49381 tacatccggc aaccaccgtt taccccgtca ccacttcacc cgccggtggc gtttccagca
    49441 gttccagcat agtacgggca atttcacgct cgcccatcac tacttgattc gcaccacgtt
    49501 cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca atatccggat
    49561 ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttgggg atcgtcagga
    49621 tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc gccgcattgc
    49681 ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt gacgtctcaa
    49741 tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg cctacacgac
    49801 cgtaacccac caatagcgca tggttgcaaa tatccactgg gatctgcttc tcttcttcga
    49861 ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat ttctccagta
    49921 gtgcgaacag tactgggttg agcataatcg acaggatcgc ccctgccagt accaggtttt
    49981 gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata aacgcgaact
    50041 caccaatctg cgccaggctg gcggcgatag ttaatgccgt acgttgggag tgaccaaaca
    50101 gtcgcaccag gaaaaacgcg gctaacgact taccaaacag aataatcgcc agcgtcgcca
    50161 gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg acggagacaa
    50221 aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca cggtgactca
    50281 gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag gagacatcaa
    50341 acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac agggtaaaca
    50401 gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga cggcgaccta
    50461 ccagcatcat aatggcgata aacgcgatca ctttgccgat ggtgatcccc atatcgactg
    50521 caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg ggcagcaaca
    50581 ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg atttgcccac
    50641 gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg
    50701 tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc acggcagaga
    50761 gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcaccggga atggcgatgg
    50821 cctttaccgc catcaaatcc ttgagcgaaa agtgcagacc cacgccaaac atcaacagaa
    50881 tgacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag cccggagtga
    50941 atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata cgtagtttat
    51001 tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg gtgataagcg
    51061 gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt ttggccggga
    51121 aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca tgaataatta
    51181 ttgaattttg cagaaaaatg gaattagctg caaaaaagca cggatcggaa aacagaaggc
    51241 ggtttaacag aggaaaggct tatggcgact gtaaaacgat gcagccaaag tttgcctttg
    51301 gctgcaatga aatcagtctt tatgccggtt atcaggcagg aatatggtca acatccccaa
    51361 tagtggcagg aaagcacata ttttatagac taactcgatg ctggtgtgat cggcgataag
    51421 ccccagaact gccgctccca gacctcccat gccgaaagca aaaccgaaaa agagtccaga
    51481 aaccataccg atacgtcctg gaagcagctc ctgagcgtag accagaatgg cagagaatgc
    51541 cgaagcgagg ataaatccaa taatcaccgt taaaaccccc gtccagtgca ggctggcgta
    51601 gggtaaaatc agcgtaaacg gcgcaacgcc gaggatagag ccccaaatca catatttccg
    51661 cccaatttta tcccctacag gcccgccgat caccgtacct gccgcaacgg caaacaggaa
    51721 ggcaaacaga tgaagctgag cattctggat agataatccg aatttttgca tcagataaaa
    51781 ggtgtaatag ctgctgatgc tcgccatata gaaatatttc gagaaaatga ggattaacag
    51841 aatgctgacc gccagtacca ctttgttgcg cggcagtgga ttgataatcg tcgctttggg
    51901 ttttccttta ttcattcggt gctgtgccga gtaccaacgg ctgatttgcg ccaacaccac
    51961 gatcgccagc agtgccgcaa gcacaaacca ggcaacgttg cctttgccat aaggcgcgat
    52021 aatcaccgcc gccagcaagg gtcccaggga actgccaaag ttgccgccga cctgaaagat
    52081 agattgtgcc aggccatgcc gcccgccgga agccatacgg gccacgcgag aagattccgg
    52141 atgaaagacc gatgaaccgg taccgaccag cgccgccgcc agcagaactg cgccaaaact
    52201 gcccgccagc gcaagcagca ccagaccact taaggtaaag cacatgccaa ttggcaacga
    52261 ccacggcatc ggatatttat cggtccagta gccgaccact ggttgcagta gcgaagaggc
    52321 gagctggaag gtgagggtta tcatgccaat ctgcataaat gtcagagaaa attctgactg
    52381 aagcagagga taaatcgcca gaatcagcga ttggatcatg tcgttcagca gatgtgagag
    52441 gctgatagca cctaaaatac caaacgatgt tcgggccttg gtcgttgacg cagccgcgcc
    52501 cgccacaggc tgggtttgtt cactcattgc cataggaaag tcactttttc agggttgcga
    52561 tgttaagaat gatcttattt gtgattatta cccgactaac atacctgtat gcgccgcctg
    52621 aaggaagtct caacgctgaa tacagaattt ctaatctgga tgcagattta tcttcaccgg
    52681 acgcagactt gtctatgatg tcgcgtcata ctatttttca acacgttgaa atcaggtcag
    52741 ggagagaagt atgaaattat tgcagcgggg cgtggcgtta gcactgttaa ccacatttac
    52801 actggcgagt gaagctgctc tggcgtatga gcaggataaa acctacaaaa ttacagttct
    52861 gcataccaat gatcatcatg ggcatttttg gcgcaatgaa tatggcgaat atggtctggc
    52921 ggcgcaaaaa acgctggtgg atggtatccg caaagaggtt gcggctgaag gcggtagcgt
    52981 gctgctactt tccggtggcg acattaacac tggcgtgccc gagtctgact tacaggatgc
    53041 cgaacctgat tttcgcggta tgaatctggt gggctatgac gcgatggcga tcggtaatca
    53101 tgaatttgat aacccgctca ccgtattacg ccagcaggaa aagtgggcaa agttcccgtt
    53161 actttctgcc aatatctacc agaaaagtac cggcgagcgc ctgtttaaac catgggcgct
    53221 gtttaagcgt caggatctga aaattgccgt tattggtctg acgacggatg acacagcgaa
    53281 gttaggcaat ccagaaaact tcaccgatat tgagttccgt aagcccgccg atgaagcgaa
    53341 gctggtgatt caggagctgc aacagacaga aaagccagac attattatcg cggcgacaca
    53401 tatggggcat tacgataacg gcgatcacgg ttctaacgca ccgggcgatg tggagatggc
    53461 acgcgcgttg cctgccggat cgctggcgat gattgtcggc ggtcactcgc aagatccagt
    53521 ctgtatggca gcggaaaata aaaagcaggt agattacgtt ccaggtacgc catgcaaacc
    53581 ggatcaacaa aacggcatct ggattgtaca ggcgcatgag tggggcaagt acgttggtcg
    53641 ggccgacttt gagtttcgca acggcgaaat gaaaatggtt aactaccagc tgattccggt
    53701 gaacctgaag aagaaagtga cctgggaaga cgggaaaagc gagcgcgtgc tgtacacccc
    53761 tgaaatcgct gaaaaccagc aaatgatctc gctgttatcg ccgttccaga acaaaggcaa
    53821 agcgcagctg gaagtgaaaa taggcgaaac caatggtcgt ctggaaggcg atcgtgacaa
    53881 agtgcgtttt gtacagacca atatggggcg gttgattctg gcagcacaaa tggatcgcac
    53941 tggtgccgac tttgcggtga tgagcggagg cggaattcgt gattctatcg aagcaggcga
    54001 tatcagctat aaaaacgtgc tgaaagtgca gccattcggc aatgtggtgg tgtatgccga
    54061 catgaccggt aaagaggtga ttgattacct gaccgccgtc gcgcagatga agccagattc
    54121 aggtgcctac ccgcaatttg ccaacgttag ctttgtggcg aaagacggca aactgaacga
    54181 ccttaaaatc aaaggcgaac cggtcgatcc agcgaaaact taccgtatgg cgacattaaa
    54241 cttcaatgcc accggcggtg atggatatcc gcgccttgat aacaaaccgg gctatgtgaa
    54301 taccggcttt attgatgccg aagtgctgaa agcgtatatc cagaaaagct cgccgctgga
    54361 tgtgagtgtt tatgaaccga aaggtgaggt gagctggcag taatccgaaa gtgccggatg
    54421 ttcgcatccg gcacaatgct taatcgcggc gggcgatatc agcaaatttg gcatcgagga
    54481 tcttcgccag atcgcctgcc gccagttcga tatccagtcc gcgcttgccg ccggaaacat
    54541 aaatagtggc aaattcttgt gcgggggcgt cgataatcgt tggcagacgt tttttctgcc
    54601 ccagtgggct aatcccccca accaggtatc ccgtcgaacg ctgcgcgacc atcggatcgg
    54661 ccatctcaac tttcttggca cccagcgctt ttgctacttt tttaaggtcc agttgacctg
    54721 cgaccggcgt aacggccacg gcaaggtgtt tcatatcacc gttcactgcg accagcagcg
    54781 ttttgtagac ctgatccgga ttcaaaccta attttttgac gacttcatcg ccaaaattgg
    54841 tttcagccgg atcgtgctcg taggtatgga tttgaaacga aatcttgttt ttttcgagtg
    54901 atttaactgc gggtgtcatg gtaattttcc tgccttaaac aaaaaaacga agcaagcata
    54961 cgcctgatgg cggtccaaaa aaaagagtca tcttgcctaa gagtattggc aggatggtga
    55021 gattgagcga caatcgagtt acaccgtcgc ttaaagtgac ggcataataa taaaaaaatg
    55081 aaattcctct ttgacgggcc aatagcgata ttggccattt ttttagcgca acatttgcgg
    55141 taaattccct tctccataca ggtgtagtgc accgaccgcg accacatatc gccccggcgg
    55201 catggcgcgt aatttatccc gccaggcgag atttcgctga tgcatcagca catcgtacag
    55261 cgactgactg aacgtattgg gcagcgttat ttcattattt tgcggcggcg cattcagcca
    55321 ccagctcatc atttgttgca gcaaccgtgc gttggtatgc cagtgggtca gcgtatcgtc
    55381 cagcagcgcc agtcctttgt cagggagctg gagcaacatg gcaatctggt tttccgcccc
    55441 ttccagttca atcacgggtt tatgttgttg cttcgccgcc tgcaatagct gataatcgat
    55501 accgtattct gcccgcagcc ccagtttttg tgcctgcgtc gcctgaagaa ccatcgcgat
    55561 ttgccacagc ggttgggtag aaaagagtga gggagaaatg cccatctcct ggctaatgtg
    55621 ctgcagtttt tggagttgct cctcgctaat gcgctcttcc agcgcctcgc aggcaggcaa
    55681 attagcaaaa ggcgtatcgc tggtggaaac atccgcctcg acgatcagcg catcggcgtt
    55741 tttgagcttt ttgagcaaac gggtgggtag gggagccata tcgtggctac ccatatgaat
    55801 actgccaatc agatgaaaat ggcgattgcc gggaagagag atatcaatgg caggccaggt
    55861 gtaatgatta ccgcgcagcg cggcccaaag cgtttttacc cggtacaaca gatccatacg
    55921 acctcccttt gtgaaatatc atgctagcgc gcggggaggg atggcgcaaa tggagttggg
    55981 gacttgttta atgtgtttgt atgattcagt atgttcttgt atcgctattt acagggaagc
    56041 aacagttaaa aactatgaaa caggcaacaa gaaaaccgac gacacccgga gatattcttc
    56101 tctatgaata tctggaaccg ctagatttga aaatcaatga gttagcagag ttgctgcatg
    56161 ttcatcgtaa tagcgtcagt gcactgatca ataacaatcg taaactcacc actgagatgg
    56221 cattccgtct ggcgaaagtt tttgatacca cagtcgattt ttggctaaac ctccaggcgg
    56281 cagttgatct ttgggaagtt gaaaacaaca tgcgcaccca ggaagaattg ggacggattg
    56341 aaacagtggc tgaatatttg gcacgccgtg aagagcgtgc aaaaaaggtc gcgtaaggcc
    56401 aaaacatcgc ttcatctcgt aggccggata aggcgttcac gccgcatccg gcatccgagc
    56461 accattgcct gatgcgacgc ttacgcgtct tatcaggcct acaaacctgt gcctgaaccg
    56521 taggccggat aaggcgttta cgccgcatcc ggcagtcatg cgtcgatgcc aaatgcgcca
    56581 ccctaaagca gcgcatccgc aatgacgtac ttattccttc ggtttaaacc gcagcaaccg
    56641 gttggcgtta ctcactacgg taatcgacga gagcgccatt gccgctccgg caactaccgg
    56701 gttaagcagt gttccagtaa acggccacaa aataccggcg gcgaccggaa taccgatact
    56761 gttgtagata aacgcgccga gcaggttctg cttcatgttg cgcagcgttg cgcgggaaat
    56821 agcgagcgca tccgcaacgc ccatcaggct atggcgcatc agggtaatcg ccgcggtttc
    56881 aatggcaaca tcactgccgc cgcccattgc aatgccgaca tccgcctggg ccagcgctgg
    56941 cgcatcgtta atgccgtcgc ccaccattgc cacctgacgt ccttcacttt gcaggcgttt
    57001 aatcgcttcg gctttaccgt ccggcagcac cccggcgatc acctcatcaa tccctgcttc
    57061 tttggcgatc gcattggcgg tggttgggtt atccccggtc aacatcacca gacgatatcc
    57121 cgctttatgc agacgttgca gcgccgccac gctatcacta cgcaacggat cgcgtacagc
    57181 cagcagggct accgctttcc cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc
    57241 ctgagctgaa atatctgctt caatcgcttt agtatcaact tgctggtcat ttaacagcgc
    57301 ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca gcccgcgcaa
    57361 tgtgcggaaa ccgttgacct gcggcagctg catatcgctt gctttatcga ggatcgcccg
    57421 cgccagcgga tggctggaac cttgttccag tgccgccgcc agacgcagcg cctgcgcttc
    57481 gtcaaaatca gcaaatgttt tcactgcgac aacctgcggc ttcccttcgg tcagcgtccc
    57541 ggttttatcg aacactacgg tatcgagtgt actagcgcgt tgcagcgcgt cggcgtcccg
    57601 caccagcacg ccaaactcag ccgcccgccc gacgccggaa ataatcgaca tcggcgtcgc
    57661 cagccccagc gcacacggac aggcaataat cagcaccgtg gtggcaatca ccagggtata
    57721 gacaatctgc ggtgccggac caaagaaata ccagattgcc gcactgacaa gcgcaataac
    57781 cactactacc ggcacaaata cggctgagat tttatccgcc agctgaccga tttctggctt
    57841 gctgctctgg gcctggcgca ccatacgaat aatccttgat agcgtggtat ggctgccaac
    57901 cgcactggcg cgaaacagaa cgctaccgtc ctgtaccact gtcccggcat ggacgctatc
    57961 gccttcgcct ttttgctgcg ggataggttc gcccgtcagc atcgcttcat ccagccatgc
    58021 ttcgccctgg gtaatctcgc catctactgg cacgcggtcg cccgtcgtca ggcgcagtag
    58081 catgcctggc tgaacttccg ccagcggcac gcttttttca ccttcgtcag taaccaggcg
    58141 cgccgtcggc ggagttaaat cgagtaattt ctccagcgcc tttgaagaac gctgacgtgc
    58201 gcgcgcttcc agcatatgac cgagattaat cagaccgata atcatcgcac tggcttcata
    58261 gtaaagatgc cgcgcttcca tcgggaacca ctgcggccac aggttgacgc tcatcgaata
    58321 gagccacgcc acacccgtac ccagcgccac cagcgtatcc atcgtcgccg caccgttcag
    58381 caggcttttc catgcactgc ggtaaaaatg gccgccggcg aaaaccatca ctgccagggt
    58441 tatcaggccg ataaccaacc acaggctgcg gttgtcagcg gtgaccatca tgttatcgcc
    58501 gatcatcccc cagaccatta ccgggatacc cacagccagt gcaacaattg cctgccagcg
    58561 gaagcgcttc atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt tcgcgtcatc
    58621 ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga
    58681 ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc
    58741 cggtacgctt tgcagcgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct
    58801 cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac
    58861 cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct
    58921 tacagatgcg tcataacccg cttgtttgat ggtttcgatc agctgttctg cactggcagt
    58981 cccggtaacg tgcgcttcag tgatagacac atccgcttgt tccacatccg ggcgctgttc
    59041 gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc
    59101 gatagtttgt gacataaaac actcctttaa gacagttttg actggctgtg ataaaggtta
    59161 aaccttccag caaggggaag gtcaagaaat taataaccca ggcgagtaaa agtccgtaaa
    59221 gattaaaaaa tcggctcgat ttgcatcagg attagacatt tatctctttg ttttcctgta
    59281 gttaagttgc gggtgctaag ttaaatcagg atgcctgaaa atcggcaccg gggtgaggaa
    59341 ttacctcccg catctataaa aaggagttaa caaaagatgt tagatgcaaa caaattacag
    59401 caggcagtgg atcaggctta cacccaattt cactcactta acggcggaca aaatgccgat
    59461 tacattccct ttctggcgaa tgtgcctggt caactggcag cagtggctat cgtgaccagc
    59521 gatggcaacg tctatagtgc gggtgacagt gattaccgct ttgcgctgga atccatctcg
    59581 aaagtctgta cgttagccct tgcgttagaa gatgtcggcc cgcaggcggt acaggacaaa
    59641 attggcgctg acccgaccgg attgcccttt aactcagtta tcgccttaga gttgcatggc
    59701 ggcaaaccgc tgtcgccact ggtaaatgct ggcgctattg ccaccaccag cctgattaac
    59761 gctgaaaatg ccgaacaacg ctggcagcga attttacata tccaacagca actggctggt
    59821 gagctggtag cgctctctga cgaagtcaac cagtcggaac aaacaaccaa cttccataac
    59881 cgggccattg cctggctgct gtactccgcc ggatatctct attgtgatgc aatggaagcc
    59941 tgtgacgtgt acacccgtca gtgctccacg ctcatcaata ctgttgaact ggcaacgctt
    60001 ggcgcgacgc tggcggcggg tggtgtgaat ccgttgacgc ataaacgcgt tcttcaggcc
    60061 gacaacgtgc cgtacattct ggccgaaatg atgatggaag ggctgtatgg tcgctccggt
    60121 gactgggcgt atcgtgttgg tttaccgggc aaaagcggtg taggtggcgg tattctggcg
    60181 gtcgtccctg gagtgatggg aattgccgcg ttctcaccac cgctggacga agaaggcaac
    60241 agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc aactcggcta taacgtgttt
    60301 aagggctgat catgatgaac acggaaggta ataacggtaa caaacccctc ggtctatgga
    60361 acgtcgtttc catcggcatt ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc
    60421 aggctgcgtt gctaatggaa gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg
    60481 cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag ctatcccagc aatggcggca
    60541 ttatcgactt ctttcgtcgc ggattaggca acggcgtctt ttcgctggcg ctctcgttac
    60601 tgtacctgtt gacgctggcg gtgagcatcg ccatggtcgc ccgtgctttt ggcgcttatg
    60661 ccgtgcagtt tttgcatgaa ggcagccagg aggagcacct tattttgctc tacgcgttgg
    60721 ggatcattgc ggtgatgacg cttttcaact ccttaagcaa ccatgcggta gggcggctgg
    60781 aggtgatcct cgtcggcatt aaaatgatga tcctgttatt gctgattatt gccggtgtct
    60841 ggtcgctgca accggcgcat atttccgtct ctgcgccccc cagctccggt gcgttcttct
    60901 cctgtatcgg gataaccttc cttgcctatg cgggctttgg catgatggcg aacgcggcgg
    60961 ataaagtgaa agatccgcag gtcattatgc cacgggcgtt tctggtggca attggcgtta
    61021 ccacgttgct ttatatctcg ctggcgctgg ttttgcttag cgatgtatcg gcattagagt
    61081 tagaaaaata tgccgatacc gccgtagcgc aggctgcttc tccgctgctc gggcatgtgg
    61141 gttatgtgat cgtcgtcatc ggcgctttac tggcgacggc ttcagccatc aacgcgaacc
    61201 tgttcgccgt gtttaacatc atggacaaca tgggcagcga acgcgaactg ccgaagctaa
    61261 tgaataaatc cctgtggcgg cagagtacct ggggcaacat cattgtcgtg gtgttgatta
    61321 tgctgatgac ggcggcacta aatttaggct cactcgccag cgttgccagc gccacctttt
    61381 tgatttgcta tctggcggtg tttgtggtgg cgatccgcct gcgtcatgat attcacgcct
    61441 cgttgccgat tcttatcgta ggtacgttgg tgatgttgtt ggtgatcgtt ggctttatct
    61501 acagcctgtg gtcccagggc agccgtgcgt tgatatggat tattggtgca atcttactca
    61561 gccttattgt ggcaatggtc atgaagcgca ataaaaccgt ataacacctc tctgcgcgca
    61621 gtactgcctg tactgcgcct ttgtctttaa ttcaaattac ctttcttatt cctttaataa
    61681 ataagtcaca aaaaaatgat atatacgcga taaatctgtg atgctaatca cttattaccg
    61741 tggattattt ttaatgtgta tgat
//
LOCUS       DAZGXS010000012        57667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGXS010000012 DAZGXS010000000
VERSION     DAZGXS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..816
                     /locus_tag="ACHKG5_000890"
     CDS             <1..816
                     /locus_tag="ACHKG5_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0867048.1"
                     /translation="PCTLFETISLFDNYSADDMQYGDMVEQDFLSLGLSDISAKVDPY
                     RLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFSFFGQYKTLIED
                     LIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLI
                     LQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVA
                     QDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            813..1304
                     /locus_tag="ACHKG5_000891"
     CDS             813..1304
                     /locus_tag="ACHKG5_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0867049.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKK
                     DNF"
     gene            complement(1346..2296)
                     /gene="corA"
                     /locus_tag="ACHKG5_000892"
     CDS             complement(1346..2296)
                     /gene="corA"
                     /locus_tag="ACHKG5_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0867050.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2448..2504)
                     /gene="ysgD"
                     /locus_tag="ACHKG5_000893"
     CDS             complement(2448..2504)
                     /gene="ysgD"
                     /locus_tag="ACHKG5_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0867051.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2666..3430
                     /gene="yigE"
                     /locus_tag="ACHKG5_000894"
     CDS             2666..3430
                     /gene="yigE"
                     /locus_tag="ACHKG5_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0867052.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            3513..4415
                     /locus_tag="ACHKG5_000895"
     CDS             3513..4415
                     /locus_tag="ACHKG5_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFP0867053.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(4460..6622)
                     /gene="uvrD"
                     /locus_tag="ACHKG5_000896"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(4460..6622)
                     /gene="uvrD"
                     /locus_tag="ACHKG5_000896"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0867054.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(6706..7422)
                     /gene="yigB"
                     /locus_tag="ACHKG5_000897"
     CDS             complement(6706..7422)
                     /gene="yigB"
                     /locus_tag="ACHKG5_000897"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0867055.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(7422..8318)
                     /gene="xerC"
                     /locus_tag="ACHKG5_000898"
     CDS             complement(7422..8318)
                     /gene="xerC"
                     /locus_tag="ACHKG5_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0867056.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(8315..9022)
                     /gene="yigA"
                     /locus_tag="ACHKG5_000899"
     CDS             complement(8315..9022)
                     /gene="yigA"
                     /locus_tag="ACHKG5_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP0867057.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(9019..9843)
                     /gene="dapF"
                     /locus_tag="ACHKG5_000900"
     CDS             complement(9019..9843)
                     /gene="dapF"
                     /locus_tag="ACHKG5_000900"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0867058.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9880..10083)
                     /gene="yifL"
                     /locus_tag="ACHKG5_000901"
     CDS             complement(9880..10083)
                     /gene="yifL"
                     /locus_tag="ACHKG5_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0867059.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10242..10384
                     /locus_tag="ACHKG5_000902"
                     /pseudo
     CDS             10242..10384
                     /locus_tag="ACHKG5_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10546..10866
                     /gene="cyaY"
                     /locus_tag="ACHKG5_000903"
     CDS             10546..10866
                     /gene="cyaY"
                     /locus_tag="ACHKG5_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0867060.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(10906..13452)
                     /gene="cyaA"
                     /locus_tag="ACHKG5_000904"
     CDS             complement(10906..13452)
                     /gene="cyaA"
                     /locus_tag="ACHKG5_000904"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0867061.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            13839..14780
                     /gene="hemC"
                     /locus_tag="ACHKG5_000905"
     CDS             13839..14780
                     /gene="hemC"
                     /locus_tag="ACHKG5_000905"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0867062.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14777..15517
                     /gene="hemD"
                     /locus_tag="ACHKG5_000906"
     CDS             14777..15517
                     /gene="hemD"
                     /locus_tag="ACHKG5_000906"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0867063.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            15539..16762
                     /gene="hemX"
                     /locus_tag="ACHKG5_000907"
     CDS             15539..16762
                     /gene="hemX"
                     /locus_tag="ACHKG5_000907"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0867064.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADTPAAAPQGE"
     gene            16765..17961
                     /gene="hemY"
                     /locus_tag="ACHKG5_000908"
     CDS             16765..17961
                     /gene="hemY"
                     /locus_tag="ACHKG5_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0867065.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            18640..20295
                     /gene="aslA"
                     /locus_tag="ACHKG5_000909"
     CDS             18640..20295
                     /gene="aslA"
                     /locus_tag="ACHKG5_000909"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP0867066.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(20463..21698)
                     /gene="aslB"
                     /locus_tag="ACHKG5_000910"
     CDS             complement(20463..21698)
                     /gene="aslB"
                     /locus_tag="ACHKG5_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0867067.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(21845..21921)
                     /locus_tag="ACHKG5_000911"
     tRNA            complement(21845..21921)
                     /locus_tag="ACHKG5_000911"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21885..21887),aa:Pro,seq:tgg)
     gene            complement(21964..22050)
                     /locus_tag="ACHKG5_000912"
     tRNA            complement(21964..22050)
                     /locus_tag="ACHKG5_000912"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22014..22016),aa:Leu,seq:cag)
     gene            complement(22071..22146)
                     /locus_tag="ACHKG5_000913"
     tRNA            complement(22071..22146)
                     /locus_tag="ACHKG5_000913"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22111..22113),aa:His,seq:gtg)
     gene            complement(22205..22281)
                     /locus_tag="ACHKG5_000914"
     tRNA            complement(22205..22281)
                     /locus_tag="ACHKG5_000914"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22245..22247),aa:Arg,seq:ccg)
     gene            complement(22384..23769)
                     /gene="yifK"
                     /locus_tag="ACHKG5_000915"
     CDS             complement(22384..23769)
                     /gene="yifK"
                     /locus_tag="ACHKG5_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0867068.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(23960..24700)
                     /gene="wecG"
                     /locus_tag="ACHKG5_000916"
     CDS             complement(23960..24700)
                     /gene="wecG"
                     /locus_tag="ACHKG5_000916"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0867069.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(24703..26055)
                     /gene="wzyE"
                     /locus_tag="ACHKG5_000917"
     CDS             complement(24703..26055)
                     /gene="wzyE"
                     /locus_tag="ACHKG5_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0867070.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26052..27131)
                     /gene="rffT"
                     /locus_tag="ACHKG5_000918"
                     /gene_synonym="wecF"
     CDS             complement(26052..27131)
                     /gene="rffT"
                     /locus_tag="ACHKG5_000918"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0867071.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(27128..28378)
                     /gene="wzxE"
                     /locus_tag="ACHKG5_000919"
     CDS             complement(27128..28378)
                     /gene="wzxE"
                     /locus_tag="ACHKG5_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0867072.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(28380..29510)
                     /gene="rffA"
                     /locus_tag="ACHKG5_000920"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28380..29510)
                     /gene="rffA"
                     /locus_tag="ACHKG5_000920"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0867073.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(29515..30189)
                     /gene="rffC"
                     /locus_tag="ACHKG5_000921"
                     /gene_synonym="wecD"
     CDS             complement(29515..30189)
                     /gene="rffC"
                     /locus_tag="ACHKG5_000921"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0867074.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30167..31048)
                     /gene="rfbA"
                     /locus_tag="ACHKG5_000922"
     CDS             complement(30167..31048)
                     /gene="rfbA"
                     /locus_tag="ACHKG5_000922"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0867075.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31067..32134)
                     /gene="rffG"
                     /locus_tag="ACHKG5_000923"
     CDS             complement(31067..32134)
                     /gene="rffG"
                     /locus_tag="ACHKG5_000923"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0867076.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(32134..33396)
                     /gene="wecC"
                     /locus_tag="ACHKG5_000924"
     CDS             complement(32134..33396)
                     /gene="wecC"
                     /locus_tag="ACHKG5_000924"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0867077.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(33393..34523)
                     /gene="wecB"
                     /locus_tag="ACHKG5_000925"
     CDS             complement(33393..34523)
                     /gene="wecB"
                     /locus_tag="ACHKG5_000925"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0867078.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(34579..35625)
                     /gene="wzzE"
                     /locus_tag="ACHKG5_000926"
     CDS             complement(34579..35625)
                     /gene="wzzE"
                     /locus_tag="ACHKG5_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0867079.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(35637..36740)
                     /gene="wecA"
                     /locus_tag="ACHKG5_000927"
     CDS             complement(35637..36740)
                     /gene="wecA"
                     /locus_tag="ACHKG5_000927"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0867080.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(36980..38239)
                     /gene="rho"
                     /locus_tag="ACHKG5_000928"
     CDS             complement(36980..38239)
                     /gene="rho"
                     /locus_tag="ACHKG5_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0867081.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(38324..38425)
                     /gene="rhoL"
                     /locus_tag="ACHKG5_000929"
     CDS             complement(38324..38425)
                     /gene="rhoL"
                     /locus_tag="ACHKG5_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0867082.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(38566..38895)
                     /gene="trxA"
                     /locus_tag="ACHKG5_000930"
     CDS             complement(38566..38895)
                     /gene="trxA"
                     /locus_tag="ACHKG5_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0867083.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39026..40291
                     /gene="rhlB"
                     /locus_tag="ACHKG5_000931"
     CDS             39026..40291
                     /gene="rhlB"
                     /locus_tag="ACHKG5_000931"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0867084.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            40427..41911
                     /gene="gppA"
                     /locus_tag="ACHKG5_000932"
     CDS             40427..41911
                     /gene="gppA"
                     /locus_tag="ACHKG5_000932"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0867085.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(41958..43979)
                     /gene="rep"
                     /locus_tag="ACHKG5_000933"
     CDS             complement(41958..43979)
                     /gene="rep"
                     /locus_tag="ACHKG5_000933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0867086.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44196..44645
                     /locus_tag="ACHKG5_000934"
     CDS             44196..44645
                     /locus_tag="ACHKG5_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0867087.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            44844..45125
                     /gene="ppiC"
                     /locus_tag="ACHKG5_000935"
     CDS             44844..45125
                     /gene="ppiC"
                     /locus_tag="ACHKG5_000935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0867088.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45171..46646)
                     /gene="ilvC"
                     /locus_tag="ACHKG5_000936"
     CDS             complement(45171..46646)
                     /gene="ilvC"
                     /locus_tag="ACHKG5_000936"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0867089.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            46796..47689
                     /gene="ilvY"
                     /locus_tag="ACHKG5_000937"
     CDS             46796..47689
                     /gene="ilvY"
                     /locus_tag="ACHKG5_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0867090.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(47741..49285)
                     /gene="ilvA"
                     /locus_tag="ACHKG5_000938"
     CDS             complement(47741..49285)
                     /gene="ilvA"
                     /locus_tag="ACHKG5_000938"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0867091.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(49288..51138)
                     /gene="ilvD"
                     /locus_tag="ACHKG5_000939"
     CDS             complement(49288..51138)
                     /gene="ilvD"
                     /locus_tag="ACHKG5_000939"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0867092.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(51203..52132)
                     /gene="ilvE"
                     /locus_tag="ACHKG5_000940"
     CDS             complement(51203..52132)
                     /gene="ilvE"
                     /locus_tag="ACHKG5_000940"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0867093.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52152..52415)
                     /gene="ilvM"
                     /locus_tag="ACHKG5_000941"
     CDS             complement(52152..52415)
                     /gene="ilvM"
                     /locus_tag="ACHKG5_000941"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0867094.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(52412..54058)
                     /gene="ilvG"
                     /locus_tag="ACHKG5_000942"
     CDS             complement(52412..54058)
                     /gene="ilvG"
                     /locus_tag="ACHKG5_000942"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0867095.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54061..54111)
                     /gene="ilvX"
                     /locus_tag="ACHKG5_000943"
     CDS             complement(54061..54111)
                     /gene="ilvX"
                     /locus_tag="ACHKG5_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0867096.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(54198..54296)
                     /gene="ilvL"
                     /locus_tag="ACHKG5_000944"
     CDS             complement(54198..54296)
                     /gene="ilvL"
                     /locus_tag="ACHKG5_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0867097.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            54649..56169
                     /gene="yifB"
                     /locus_tag="ACHKG5_000945"
                     /pseudo
     CDS             54649..56169
                     /gene="yifB"
                     /locus_tag="ACHKG5_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(56194..56532)
                     /gene="maoP"
                     /locus_tag="ACHKG5_000946"
     CDS             complement(56194..56532)
                     /gene="maoP"
                     /locus_tag="ACHKG5_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0867098.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            56651..57490
                     /gene="hdfR"
                     /locus_tag="ACHKG5_000947"
     CDS             56651..57490
                     /gene="hdfR"
                     /locus_tag="ACHKG5_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0867099.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(57586..57661)
                     /locus_tag="ACHKG5_000948"
     tRNA            complement(57586..57661)
                     /locus_tag="ACHKG5_000948"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57626..57628),aa:Trp,seq:cca)
BASE COUNT        14243 a        15481 c        14555 g        13388 t
ORIGIN      
        1 ccctgtacac ttttcgaaac tattagttta tttgataatt attctgctga tgatatgcaa
       61 tatggtgata tggtagaaca agattttctc tcactcggcc taagcgatat ctcagcaaaa
      121 gttgatcctt acagacttat taaataccat ttccctggcc caggttctat taatgttgct
      181 tttagtgcct caagttccgg aacaaaaata tcacaacgtg aatgcactga tattttattt
      241 gcagaaatga aagaactagc aaaaatgttt tccttttttg gacaatataa aacacttatc
      301 gaagatctga ttgaacattt tagatatgga aatggcagca attttcatag ccaacaatta
      361 aatttatctt ttcatgaaaa aataaataaa tatggttata atagtccaat acgaataatc
      421 aaagaatgta tcgaaaatgg cattaattca actccctcga caggatacca acctcttatc
      481 ctgcaaagca ttaaaaccaa attactttca tcaagactaa ataaatttaa tgacttcgag
      541 gacagtttta atggcttagg aatcagtgtt catgatattt cagctcaaaa aatatctctt
      601 ttaagttttc aaaaatacgc tataggatgg agtgcaacaa ttcattttgt agcacaggac
      661 cacttcggtc ttgatgtaac tgatatcaaa aacaagactt acagcaaata tagatttttt
      721 cgcatatggt tctttttaca acggcataaa gactttgctt tcaaaccttt tttcaccaat
      781 tttaacacta tcgaaagaat cgaaaattat ctatgaaaag caaactccaa aaaattatat
      841 tatgtttatt tcttctatgc tgcatctata atttatggac attgcgtcct gtacagattt
      901 tgtatacata ctctgatgct ggcaattcag tctttctggt cgttgatcat ctgccatgga
      961 ctgacagcga taaaataaat tggtatctga agcatcagaa tgaaattaaa aatcaacatc
     1021 ccctaccaga aggtagttgg catacctggt atgtcattga tatcggtaat ggcttcactg
     1081 actataaaaa atatatcgaa gggccgtatg aagatctcta ctgtttcccg acaattaaga
     1141 gcaatgataa ttgcatcacg aaaaattact taatggttat taatgaatac ccttatcgta
     1201 acactcatat tggtattaac gattttactg agtatcaatt aacacaagaa aataaaatag
     1261 agcaagtttt caatccgcat aactttaaaa aagacaattt ttagaaataa gccggataaa
     1321 aaaatagctt tatccggcac tcatattaca accagttctt ccgcttaaag tacagatacg
     1381 gtgccaggcc cgcgaggatc ataaagataa tcgcgcctgg gtagccgaaa ctccacttca
     1441 gttctggcat aaactcaaag ttcatcccgt agctggaagc aaccagcgtt ggcggcagga
     1501 acacgacgga taccaccgag aaaattttga tgatgcggtt ctgctcgatg ttgataaaac
     1561 ccattgccgc ctgcatcagg aagttcacct tctggaacag ggattcgtta tgcggcagca
     1621 gggattcgat atctcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaagcgtg
     1681 ctttacgtac caggaagttg agcgcgcgct gggtatccat cagacacagg cgcactttcc
     1741 agccgatatc ttccagttcc gccagagtgg agagcgcctc gtcgtactca tcgccctgat
     1801 gcccttccat aatcacccgg ctcaattgct ccaggtcgct atagatattt tcaatttcat
     1861 ctgccaactg ttcgattttg gtttcgaaca gatccagcag caactcgtag gcgttaccgt
     1921 ctaccatcga ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct
     1981 cacgcagagt aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt
     2041 gatcttccgc atcttcaaag aagaagaagg agtgaatatg caggccgtca tcgtcttcaa
     2101 agaaacgtgc cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa
     2161 gttcagattg tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg
     2221 catttacaag gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt
     2281 gaaatgcgct cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc
     2341 acacagaaac aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa
     2401 ttgaggtcgc tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc
     2461 ctggatgaca ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta
     2521 gagtgatcgt gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac
     2581 cacagaacaa tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa
     2641 aaaaatccca catccaggac ctataatggc gcatcggcta ctcattggta aaggaatgat
     2701 caccttgaat ctcaaacgga tttttctcgc cctcacctta cttcccttgt ttgctgttgc
     2761 cgctgatgat tgcgcactct cagatccaac gctgaccgta caggcgtata ccgttaatcc
     2821 tcaaacagag cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacttt
     2881 acatgctctg ctggcggata tgaatagtca gggtcaggtg cagatggcga tgaacggcgg
     2941 catctatgat gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcagaaggt
     3001 ggcgttaaat ctcgcttcag gtgaagggaa tttctttatc cgtcctggcg gcgtgtttta
     3061 tgtcgcggga gataaagtcg gcatcgttcg tctggatgcc ttcaaaacca gtaaagagat
     3121 tcagtttgcg gtgcagtcag ggccaatgtt gatggaaaac agtgtaatta atccgcgtat
     3181 tcatcccaac gtcgcctcac gtaaaattcg taacggtgtt ggaattaata aacatgggaa
     3241 tgccgtgttt ttgttgagcc agcaggcgac aaatttttat gattttgcct gttatgccaa
     3301 agcgaaactg aacgttgagc agctgcttta tcttgatggc acgatttcac atatgtatat
     3361 gaaaggcggg gcaatcccgt ggcaacgcta tccttttgtc accatgattt ccgtcgagcg
     3421 gaaaggttaa gcattgtaca ctcgaggagg ccagacaaac tggggctggc tacacagctg
     3481 aaaaatcatc agataaatga agggaaacaa aaatgaaaaa aacgctcgca acgttatttt
     3541 tattaacgtg tctgggatca tcatccgctt atgcggataa cgctttgatt ttacaaactg
     3601 attttagcct gaaagatggt gccgtctcgg cgatgaaagg ggtcgcattc ggtgttgacc
     3661 ataatcttaa aatatttgat ttaacacacg aaattccacc gtataacatc tgggaaggcg
     3721 cttaccgcct gtatcagacc gccagttact ggccgcaagg ttcggtattt gtcagtgtgg
     3781 tcgatccggg cgtaggcact gaccgtaaat cgatagtgct gaaaaccaaa aacggtcaat
     3841 acttcgtttc accggataac ggtacgctca ctttggtggc ggaatcgctg ggtattgaga
     3901 gcgtccggga aatcgacgaa aaagctaacc gcctgaaagg ttctgaaaag tcatatacct
     3961 tccacggtcg tgacgtgtat gcctacaccg gtgcacgtct ggcttccggg gcgatcacgt
     4021 tcgagcaggt ggggcctgag cttcctgcaa aggttgtcga actttcttat cagaaagcaa
     4081 aagcaacaaa aggtgaagtg aagggcaata tcccgattct cgatattcag tacggcaacg
     4141 tctggagcaa tatcagcgac gagttgctga atcaggcggg gatcaaactc aacgacacgt
     4201 tgtgtgtgac catttccgaa ggttcccggc aaaaatacgc agggaaaatg ccgtatgtcg
     4261 caagcttcgg cgatgtgccg gaaggacagc cgatggtgta tctgaacagc ttattgaatg
     4321 tttccgtggc gctgaatatg gataatttcg cgcagaaaca tcaggttgcg tctggtgctg
     4381 actggaatat tgatgtgaag aaatgtgata agtaaggtgc tgatgccgga tgcggtgtgg
     4441 cgcaccgcat cccgcaacgt tataccgtct ccagccgggc gtatgccgcc accagccatt
     4501 taataccctg gccctggaat gccacctgca aacggctatg ctcgccgctg ccttccatat
     4561 tgacgatagt gccttcacca aacttagcgt ggcgtacacg ctggccgagc ttgtagccgc
     4621 tgtcgttctc gaccatcggc gtacccatac gctgatggct gaccgggcgg cttaccgtgg
     4681 cgcgcaggcg cacctcttcc acacactctt ccggcaactc accgataaag cgcgacgggc
     4741 gatggtaaac ctctttgcca tacagacggc gagtttccgc gtaggtcagc gtcagtttct
     4801 gcatcgcgcg ggttacacca acgtaggcca gacggcgttc ttcttccaga cgtccgcctt
     4861 catccagcga catctggctt gggaacatgc cctcttccat accgacgata aacacctgcg
     4921 ggaactccag gcctttcgcc gagtgcagcg tcatcaactg cactgcatcc tgccaggtat
     4981 ccgcctgccc ctcgcccgct tccagcgccg catgggagag gaacgcctgc agcggcatta
     5041 aatcttcgtc ttcttcgttg tagctgaatt ggcgcgttgc cgtcactagt tcctctaagt
     5101 tttcgatacg cgtctgacct ttttcgccct tctcctgctc gtacatggta cgcaggccgg
     5161 agtctttaat tacccggtca gtctgtacat gcagcggcat atcggcagtt tcctgcgcta
     5221 aggcgtcgat cagttccata aaccgctgta aggcgctggc agcacgtccg gcgagggctt
     5281 tttcctgcaa cagttcacga catgcctgcc agagtgttaa ctggcgatcg cgcgatgtct
     5341 gacgtaccac gtccagcgtc cggtcaccaa taccccgcgt tggcgtattc accacacgct
     5401 caaaggccgc gtcgtcgttg cggttggcaa tcaggcgcag atacgagagc gcatctttga
     5461 tttcctggcg ttcgaagaag cgcatcccgc cgtaaatacg gtacggcata ctggcctgca
     5521 ataacgcctc ttcgagcacc cgcgactggg cgttgctgcg gtagagaatg gcgcactcgg
     5581 cgagcgcccc accgttgtcc tgccaggttt tgatgcggtt aacgacaaaa cgcgcttcat
     5641 cgagttcgtt aaaagcgcaa tagagggaaa taggctcacc gtccgcgcca tcggtccaca
     5701 gttttttacc cagacgcccg ttgttgtttt caatcagggc gttcgcggcg ctcagaatgt
     5761 tactggtaga acgatagttc tgctccagac ggatagtttc ggcaccgggg aaatcattga
     5821 ggaaacgctg aatattctcc acctgcgccc cgcgccagcc gtagattgac tggtcgtcat
     5881 caccgacgat catcactttg ccggtgtcgc ccgccagcag gcggatccac gcgtactgaa
     5941 tgttgttggt atcctggaat tcgtccacca ggatgttggt aaaacgttcg cggtagtgtt
     6001 gcaggatatg cggcttgtta agccacaact cgtgagcgcg cagcagcagc tcggcgaagt
     6061 ccaccaggcc tgcacgatcg cacgcttcct gatacgcctg atacaccttc tgccaggtct
     6121 gctccaccgg attaccgtag ctttgaatat gatgcggacg caggccttca tctttctggc
     6181 tgttgatgta ccacattgcc tggcgcggcg gccactgctt ctcgtcgagg ttcatggctt
     6241 tgatcagacg cttaagcagg cgtagctggt cttcgctatc gaggatctgg aaatcctgcg
     6301 gcagattggc gtccatatgg tgcgcacgca gcaaacggtg cgccagcccg tggaaggtgc
     6361 cgacccacat gccgccctgg ctggtgccca tcagttgccc gatacgatga cgcatctccg
     6421 ccgccgcttt gttggtaaac gtcacagcca taatcgagta tggcgagcag ttttccacgc
     6481 tcatcagcca ggcgatacga tgcaccagta cgcgcgtctt accactgccc gcgcccgcca
     6541 gcaccagaag gttgctgcgc ggcgcggcca ccgcttcgcg ctgtttgtca ttaaggctgt
     6601 cgagcaggta agaaacgtcc attggcaccg ccgcgtaaaa ataggttggg cggagaagcg
     6661 caacgccctc cgccaaaaag ctgggtatat atacagattt gctgattata tcagcgaggt
     6721 cagagatgcc aaccgggaaa tttccagatg cgggagtaaa cggctatccc aggtttgcat
     6781 cagatcgcca ttttccggtc tgatccaaca ggcctgcatt ccgctgcgaa ttgccccacc
     6841 cacgtcagtg gtgagatcgt cacctacatg taagatctcg ccgatcggca cgttgagttt
     6901 ttccgccgcc aggaagtaca tatcgctgaa cggtttcgag cgcccgtgcg ggccagcgcg
     6961 cagcacaaac tcaaaataat cccccaaacc aaacagttcc ggctgagcgt taccgttggt
     7021 gatcgccacc agcggccatt tcttcgccag ctgttttaag gtgtcgtggg tttgctgcgg
     7081 gacgtcgatc ctgctgcgcc atttagcaaa gttgatcatt gccgcgtgtg cgcctgcact
     7141 ggcttcttcc gcgctcaatc cggcgtcaag catcgcttgt tctattgagc gaaaacgcca
     7201 gcgcgtcacg tcgtgataaa tctcgggttc cgcttcccgt accgcctggc gcaggcgttg
     7261 cagatcttca ttctggaagc tgcgcagcgc cggatggtaa ttttgcacaa aggtaagcgc
     7321 ctcctgctcg gtgcgcaaaa tcaccggacg gttatcgtaa agggtatcat ccaggtcaaa
     7381 ggtgagcgcc gagatgcgcc ccaaaggccg gtaaaaacgc attatttccc ccgtttggcg
     7441 cgtggatgcg ccgcatcgta caccgaggca aggtgttgaa aatcaagatg agtatagatt
     7501 tgcgtggtgg agaggttggc atgacccagc agctcctgca caccacgaag atcgccgctc
     7561 gactccagca tatgcgtagc gaacgagtga cgtaatttat gcgggtgaac gtgattattc
     7621 agcccttgtt ttatgcccca ttcggcaaag cgtttctgca cattacgcgc ggagatacgc
     7681 ttgcccagtt tcgacagaaa aagcgcgtcg tcttcgctac caaacaggtc gcgcaaatca
     7741 agccagtgct caatccatga caaagcgtta cgaccaatcg gcaggcggcg ctctttgctg
     7801 cctttcccca tcacccacac ttcgccggac tccaggtcga ggtgtttgat gtccagcccc
     7861 accagctcgg aaagacgcag gcccgcgccg tacatcactt ccagcattgc acggtcgcgc
     7921 acagcgaggg gatcgttgat atcaatatcc aacaggcgat ttatatcatc gacgtcgata
     7981 tttttcggca gatgacgtgg cgttttcggt gccgaaacac ctttcgccgg gttagctttg
     8041 agttcgttct ggctgaccag ccagtcaaaa aagctacgta gcgcagagag gcgtaatgcg
     8101 aggcttgccg cgcccagccc tttacggcga ctgcgtacag caaaattgcg caccatcgct
     8161 gcatcgcatt gctgccagtt ttgcaggccg ttttcgctgg caaaattgat gatcgcctca
     8221 agctgtcgct ggtagttaag cagagttatc ggactaagct gccgctccac gctcagataa
     8281 cgtaggtagc gttctacatc ggtgtgtaaa tcggtcatac gcgttcaatc caacgctcca
     8341 gaagctccgg caacatcagc gcaatttcat gaagtaactg cgttccctgc ccttgttgat
     8401 agtgactggc atcgcggctg gtgaacagca ccacgcctaa atcagcatcg cttcccagca
     8461 tcgacatcgc caccgatccc accgctttcg cttccggtag taccaccagc agctccggtc
     8521 cgttaagcgg ccccagataa tgctgctcct gccccagacg ctgaatacgc agcggttcga
     8581 aagactgacg gcttaatgcc agatgagtgt ggttcgacgg cgcacctaag cgccagcgat
     8641 ccgggaacag gcgcagactc gcacctgcca ggccgagatc acgcgcccag cggtgaaagc
     8701 gcatcagcat atcatcaaga ctgctggcgg cggtgagact gcgctgcagg tagagtaagc
     8761 gataaaacag gccttcgttg gcgatagcct gttccatcaa cagcgccatg ttctcttcca
     8821 gaacatgaat atgattacgt gcgcgggcca tgtgccactc gaccaacgaa acggtaccgc
     8881 gtaccggatg cggcacacgt atcgcttcta ctgcgcgcgc attgcggata aaaaactcag
     8941 gatttttaat cagataatcg acaaccaccc ggtcatcaag ctccgtgagt gtttcctgca
     9001 gttcttcccc tggttgcttc atagatgaat aaatccgtcg tagacatgta ccgccgggcc
     9061 agtcatatat aacgggtgac ccggaccttt ccaggcgata tcaagacgac cgcctgggag
     9121 ttccacgcgt acttcttcgg ccagcaaacc ttgctgaatc cctactgcaa ccgccgcaca
     9181 tgcgccgctg ccgcaggcct gggtttctcc tgccccacgc tcataaacgc gtaaacgaat
     9241 atgctcgcgc ttaaccactt gcataaaacc gatattggcg cgctccggaa aacgctcgtg
     9301 gctttccaga acaggcccca gtgtttctac cgccgcagta tcgacatcat cgacctgaat
     9361 cacgcaatgt ggatttccca tcgacaccac gccgcataag attgtctgct cggcggcgcg
     9421 cataatatag gtcttttccg ctttgttagc gcgaaacggc acggcagaag gttcgaagtt
     9481 gggttcgccc atatttacgc ggaccagatc atcatcggtg acggtaagaa ccatccgccc
     9541 gttggcggtg ctgacgcgga tatcgcgctt attggtcagc cctttcagac gcacaaaacg
     9601 ggcaaagcag cgtgcgccgt tgccgcactg cgccacttca ctgccatcag cattgaaaat
     9661 gcgatagtga aaatccagtt caggatcata cggcggctca accaccagca gttggtcaaa
     9721 ccctaccccc aggtgccgat cagctaggcg acgaatcagc tccggtgaaa aaaagacatt
     9781 ctgcgttacc gcgtcgacga ccataaaatc gttgccaagg ccatgcattt tcgagaactg
     9841 catcatttac tccaatcacg cgggtacaga aactgacttt tagtaattca cctgggatgg
     9901 accatcgcca gtggcgcgat cgtttttatc cggcaccgtg gattgcgttt gcgtctctac
     9961 cggtttggtc ggcggcggtg catttttatc tgcaggcggg aaatagagcg gacctttcag
    10021 accgcagccc gtcaggctga agagagtaag taatacagtg agtgccttaa acacgttttt
    10081 cattatggat tgcctgtaaa gttcatcgct ttctgctttc tatcatcgca ggagaaggcg
    10141 taaaagcaag cgtttagcga ctctttatga agtctgaaaa gtgaggaaat gctgcgagta
    10201 taacgcttac tcgtgaggat ttatgttctg ctggcagttt cgtgatcaac acctcacgcc
    10261 aggatggctt tatgcactat cgcaatgtgg tttgtttgtt ggctttttta gttccgtcag
    10321 cgtgaggatg ctcgctcggt aaacctgatc ataatgttta ccagaaacag ggaaaggcgt
    10381 ggtgtaactg cgccctaatg aagagacaaa tctttcgcta cacagagaca tcattattcc
    10441 ggtgacggat gaatcatgcc gtaacgcagc cgcagaggtg caaactttga agccctgacc
    10501 ccatgccgct atactgccgc catcacttaa aaacaggata caacaatgaa cgacagtgaa
    10561 tttcatcgcc tggctgatca actgtggctg accattgaag agcgtctgga cgactgggat
    10621 ggcgacagcg atatcgactg cgaaatcaac ggcggcgtgc tgaccattac ctttgagaat
    10681 ggcagcaaaa tcattatcaa ccgccaggaa ccgctgcacc aggtatggct ggcaaccaaa
    10741 caaggcggct accattttga tctgaaaggc gatgagtgga tttgcgatcg cagcggcgaa
    10801 accttccggg atttgctgga acaggcggcg acgcagcagg cgggtgaaac agtcagtttc
    10861 cgctaagatt gcatgccgga taagcctcgc tttccggcac gttcatcacg aaaaatactg
    10921 ctgtaatagc ggcgtatcgt gatcctgatt ggcaggcggc atgttaccga tagattttgt
    10981 gcggaacgga atcacctgtt cacgaccatc aaccttcaca atctgataga actgcggcag
    11041 gttgaagttg atgaagcttg agccgtaggt aaaacggtca tgcgatgacg agtagaagcg
    11101 actgacgtca cgtaccagct cctctttgct gccttcgcag tggtgataca cctcaacccg
    11161 attgctttcg tcgagaatgt agatattaaa gccattctca tcttgcgttt cttcgaaaaa
    11221 gaactggatg atcccttcgc tggcaaagcc gtccaccacc gccggtaatt tgacgtgatt
    11281 ggtttcaacc tgcactgaca ggccgtgcag tttgttatgc gaaatagcgc cataaaactc
    11341 gatggcgttc tccagtttct gtaccgatac attcaggcgt tcgaagaaca agccccaggt
    11401 ttgaccagaa acgcgcagcg ccttgaaacg cccggtttcc tggcgggtgc tggaaagacg
    11461 caattcaata cactcagaaa ccagttgctg cacgcgagta cgaattaagc cgcgcagatg
    11521 ctggctataa cagaagactt ccacgctatc tggcggtgcg gcgtcctgat gcattttgcc
    11581 gagaatagtt ttcagggctt cgatcatcga ttgctcgccg ttgaagtgca gcgtacgcac
    11641 ttcattccac gagttgcgat acagcaggtc aacgctacct accaggcaat tttgattctc
    11701 gccaaagctg aagacatcca gcttacggaa atcaaaatgc accacctgat tgcggaacgc
    11761 agctgtcggg tcatattcca ggttaacgat aatcgccaga tggcggatct cacacgggct
    11821 gtagagcgcc ttcggtgtcg gtgcaggtaa gcgcaacggg aaatggtgcg acacgtcggc
    11881 gaccatctcc tgcaacttag gcaaatcgac aatgccgtta cctttaatat acaagcgggt
    11941 gcgcgaggtc agcaggccgt taaaccatgc ccacgccacc agtttattca ggtaacggtt
    12001 atattccagc ggctgatggc tgatgatcga ctcaatattt ggcgcgcggt tatacagata
    12061 ccaacctgaa cggttagccc ggcccggcgg cacataaata aaggtcagat tcggttccga
    12121 gagatcgggt gaaatctgcg ggtttaccag cgtcacttta cctggtaatg cttcaaacgc
    12181 ggcatacagc ttacgcgtca gcacgccgat atcctgcgga ctggcggaga cgctaaggtt
    12241 attgcgacgc gcaaagcgga tcagattacg gtagctctgc atcatcgcgt cgagcaactc
    12301 gttgtgcgcc tcacgcacct gatcaatctt ccagttagcg cggttatcga gcattgccag
    12361 acgagcttcg tcccaccccc actcgctcac taactggctc aacactgcgc gacgccagcc
    12421 tacgcaggcg cgttcacggc tgagcttttc gcacactttt aaatagaagc agcgacgtac
    12481 taaatccaga cgggtgaaat cttcaatcgc cgtcaggtat tcagtaacac gctccagcat
    12541 catgcagtaa ggatcgaggc caaacgatac aatctcgccg tcgtgcaaac gctgtttgat
    12601 atctttcgcc agcaggcgtg ggttcgggta ttcccaggaa taggcttcca gcagcagtgt
    12661 cttcagcacc gctttgtatg gagaatcgat actcttgtag agttgccaaa ggctggcacc
    12721 gaagtattct tcagcggaaa gcgagcttaa gccgccgaga tccagccatt catttggcgt
    12781 cagcacgcct tgtgcgtaga gcgtcattac gtagtcatcg taatgctctt cttcgtcgca
    12841 cggcaccata ttccacagaa tacgcttacc ggcgagacgc acggcggtac gataaaattc
    12901 atcaagcagc agtatatgct gggtggagcc acaatcttcg ccccccagac tgccactctc
    12961 gttgtggcga aaacggtttt catcaatcag gaagaagcta acttccacac ccagcgaggc
    13021 ggcccagctt tccagcaggc tgcatttacg ttgtagcaac tggcgctctt cgctatcgag
    13081 ccaggattga tgacagaccc agatatccag gtcagaggaa caactttgcc ctacggacga
    13141 ggtgctgccc atggtgtata caccagtaat tggaagctca cctttcggcg ggtcctgtac
    13201 tgacattcca cgatacagtt caagctcgtt caggtagtgg cgttgagttt catcaggcgt
    13261 gtaaaggcaa atgcctttgg gaacgttacc atcaaggtaa cccggcatta gcggatggtg
    13321 atagtgcaac aatgtcggca gtagactgta gacctgttgg aatgcaggcc ccatagcagc
    13381 aagcgcgcga tccacacgca attgatttat ggcatccagt ctctgtttca gagtctcaat
    13441 atagaggtac aagacgtatc gcctgatttg ctacccgtca tgactgtgat tccgccaaca
    13501 tcaacggtaa cacgcggcat tcgggatatt tcgtatgtca aaggtaaccg ttaccacttt
    13561 tcgcgcctgg tttttttagt ttcacgacga aaaaatggtc taaaacgtga tcaatttaac
    13621 accttgctga ttgaccgtaa agaaagatgc gctacataca agtgtagcac cgtttattct
    13681 ctgtaaattc cttattacga cggcgtgaaa cgcctgtcag gatccactgc cagacctcat
    13741 tttacggttt gcgcaggcgt ctacgtttca ccacaacact gacatcactc tggcaaggat
    13801 gttaggatgg accacggatg ataatgacgg taacaagcat gttagacaat gttttaagaa
    13861 ttgccacacg ccaaagccca cttgcactct ggcaggcaca ctatgtcaaa gacaagttga
    13921 tggcgagcca tccgggcctg gtcgttgaac tggtaccgat ggtgacgcgc ggcgatgtga
    13981 ttcttgatac gccgctggcg aaagtcggcg gaaaaggctt atttgtaaaa gagctggaag
    14041 tcgcgctcct cgaaaatcgc gccgatatcg ccgtacattc aatgaaagat gtgccggttg
    14101 aattcccgca aggtctgggc ctggtcacta tttgtgagcg tgaagatcct cgcgatgcct
    14161 ttgtgtccaa taactatgac agtctggatg cgttaccggc aggcagtatc gtcgggacgt
    14221 ccagtttacg tcgccagtgc caactggctg aacgccgccc ggatctgatt atccgctccc
    14281 tgcgcggcaa cgtcggcact cgcctgagca aactggataa cggcgaatac gatgccatca
    14341 ttcttgccgt agccggacta aaacgtttag gtctggagtc ccgcattcgc gccgcgttgc
    14401 cacccgagat ttctcttccg gcggtaggac aaggtgcagt cggtattgaa tgccgccttg
    14461 atgatacacg cactcgcgag ctgcttgccg cgctgaatca ccacgaaact gcactgcgcg
    14521 ttaccgcaga acgcgccatg aatacccgtc tcgaaggtgg atgtcaggtg ccaattggta
    14581 gctacgccga gcttattgat ggcgaaatct ggctgcgtgc gctggtcggc gcgccggacg
    14641 gttcgcagat tattcgcggt gaacgccgcg gtgcgccgca agatgccgaa caaatgggga
    14701 tttcgctggc agaagagcta ctgaataacg gcgcgcgcga gatcctcgct gaagtctata
    14761 acggagacgc cccggcatga gtatccttgt cacccgcccg tctcccgctg gagaagagtt
    14821 agtgagccgt ctgcgcacac tggggcaggt ggcctggcat tttccgctga ttgagttttc
    14881 tccgggtcga caattaccgc aacttgctga tcaactggca gcgctggggg agagcgatct
    14941 gttgtttgcc ctctcgcaac acgcggttgc ttttgcccaa tcacagctac atcagcaaga
    15001 tcgtaaatgg ccccgactac ctaattattt cgccattgga cgcaccaccg cactggcact
    15061 acataccgta agcggacaga agattctcta cccgcaggat cgggaaatca gcgaagtctt
    15121 gctacaatta cctgaattac aaaatattgc gggcaaacgt gcgctgatat tacgtggcaa
    15181 tggcggtcgt gagctaattg gggataccct gacggcgcgc ggtgctgagg tcactttttg
    15241 tgaatgttat caacgatgcg caatccatta cgatggtgca gaagaagcga tgcgctggca
    15301 atcccgcgag gtgacgacgg tcgttgttac cagcggtgaa atgttgcagc aactctggtc
    15361 gctgatccca caatggtatc gtgagcactg gttactacac tgtcgactat tggtcgtcag
    15421 tgagcgtttg gcgaaactcg cccgggaact gggctggcaa gacattaagg tcgccgataa
    15481 cgctgacaac gatgcgcttt tacgggcatt acaataactc tcataacagg aagccataat
    15541 gacggaacaa gaaaaaacct ccgccgtggt tgaagagacc agggaggccg tggacaccac
    15601 gtcacaacct gtcgcaacag aaaaaaagag taagaacaat accgcattga ttctcagcgc
    15661 ggtggctatc gctattgctc tggcggcggg cgtcggtttg tatggctggg gtaaacaaca
    15721 ggccgtcaat cagaccgcca ccagcgatgc cctggctaac caactgacgg cattgcaaaa
    15781 agcccaggag agccaaaaag tcgagctgga aggcattatt aagcaacaag ctgcacagct
    15841 taagcaggcg aatcgtcagc aagaaacgct ggcaaaacag ttggatgaag tccaacaaaa
    15901 ggtcgccacc atttccggca gcgatgctaa aacctggctg ctggctcagg ccgattttct
    15961 ggtgaaactc gccggacgga agctgtggag cgatcaggac gtcacgaccg ctgcagcgtt
    16021 gctgaaaagt gcagacgcca gcctggcgga tatgaatgac ccgagtctga ttaccgttcg
    16081 tcgggcaatt accgatgata tcgccagcct ttctgcagta tcgcaggtgg attatgacgg
    16141 catcatcctt aagcttaatc agctttcaaa tcaggtagat aacctgcgtc tggccgataa
    16201 tgacagcgat ggttcgccga tggattccga cggtgaagag ctttccagtt ccatcagcga
    16261 atggcgtatc aatctgcaaa aaagctggca gaactttatg gacaacttca ttacgattcg
    16321 ccgtcgtgat gacaccgccg taccgctgtt agcgccaaat caggatatct atctgcgcga
    16381 aaatattcgc tctcgcctgc tggtcgcagc acaagctgta ccgcgtcacc agcaagagac
    16441 ttatcgccag gcgctggaga acgtctccac ctgggtacgt gcttactacg atactgatga
    16501 tgccaccacc aaagcgttcc tcgacgaggt agaccagtta agccagcaaa atatctcgat
    16561 ggatcttccg gaaaccctgc aaagccaggc gatgctggaa aaactgatgc agactcgcgt
    16621 gcgtaacctg ctggcacaac cggcagcggg gacaacggaa gctaaacctg cacctgcacc
    16681 tgcacctgca cctgcacctg cacctgcacc tgcacctgca ccgcaagctg atactccggc
    16741 agccgcgccg caaggagaat aaccatgcta aaagtgttat tgctctttgt gttgctgatt
    16801 gcggggatcg tggttggccc gatgattgcc ggtcatcagg gttatgtgct gatccagacc
    16861 gacaactaca atatcgaaac cagcgtcacg ggcctggcga tcatattgat tctggcgatg
    16921 gtagtgctgt ttgccattga gtggctactg cggcggatct tccgcactgg cgcgcacacc
    16981 cgtgggtggt ttgtcgggcg taagcgtcgc cgtgcacgta agcagaccga acaggcgctg
    17041 ctgaaactgg cggaaggcga ttatcagcaa gttgaaaagc tgatggcgaa aaatgccgat
    17101 cacgctgaac aaccggtggt gaactatcta ctggctgccg aagccgcgca acaacgtggt
    17161 gatgaagcgc gcgccaacca acatctggaa cgcgcagcgg agctggccgg caatgacacc
    17221 attccggtag aaatcacccg tgtacgtctg caactggccc gtaatgaaaa ccatgctgca
    17281 cgccacggcg tggataagct gctggaagtt acgccacgcc atccggaagt attacgtctg
    17341 gcggaacagg cgtatatccg cacaggtgca tggagttcgc tgctggatat tatcccatca
    17401 atggcgaaag cccatgttgg tgatgaagaa catcgtgcaa tgctggaaca acaggcatgg
    17461 attggcctga tggatcaggc gcgtgccgat aacggtagcg aaggtttgcg taactggtgg
    17521 aaaaaccaaa gccggaaaac gcgtcatcag gtagcgttgc aggtggcaat ggcggaacat
    17581 cttattgaat gtgacgatca tgatactgcc cagcaaatta tcatcgatgg cctgaaacgc
    17641 cagtacgacg atcgcctact gctgccgatt cctcgactga aaacaaacaa tccggaacag
    17701 ctggaaaaag tgctgcgcca gcaaatcaaa aacgtcggcg atcgcccgct gttgtggagc
    17761 acactgggcc agtcactgat gaagcacgga gaatggcagg aagcatcgct cgccttccgc
    17821 gcagcgctga aacaacgtcc ggacgcctac gattacgcat ggcttgccga cgcgctggac
    17881 agactgcaca agccggaaga agctgcagcg atgcgtcgcg acggtttgat gttaacgttg
    17941 cagaacaacc caccacagta gttccttctc acccggaggc aagcacctcc gggaccttcc
    18001 tgacacataa aaaaacgcct gctcttatta cggagcaggc gttaaaacag gtctgtatga
    18061 caacaagtgg gtgcttcact caacgttgtg tccatggtgt ctgatgaggc ataagcgaca
    18121 tctgtcagtg gacgataagc accgtaaacg gctctgcgtc attccggagt ttatgaggca
    18181 ctaaggcgaa cataagagat ggaatgagca tctactcgtt tattatgcca cagagaatcg
    18241 ggaaataaca tcccttaaca cttgttatga gataattctg taatcctctt tgcttcctga
    18301 gtaataactt cctgagtgaa tatttaacct gagcttgatc ctacacatac ttattatgaa
    18361 tgataaaatt cattcaatta ataacacata tattaattgc cgttaaaact aaaaacagca
    18421 tcaataatca acgcgatata ataaacctgc cttacatatc aactgcgcca gaggtaggat
    18481 tgaaaacgct ctcctgattt ttaaattcat ttcttggata aataaataat ttatttttat
    18541 cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca atgcttaatt
    18601 acgtcatcat tttgttgaca tgaaaccgga gcattttgca tggaattttc gttttcaccc
    18661 aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc agcagatacc
    18721 ccgtcaactg ccaccgcgcg caaaggcttt gccggatacg atcacccaaa ccagtatctg
    18781 gttaaaccgg cgaccactat tgccgacaac atgatgccag taatgcagca tccggcgcag
    18841 gataaagaac cccagcagaa gctggcagaa cttgagaaaa aaaccggtaa gaaaccgaat
    18901 gtggttgttt tcttgctgga cgatgtgggc tggatggacg tcggttttaa cggtggcggc
    18961 gtggcggtgg gtaaccctac accagatatc gacgccgttg ccagccaggg gctgatttta
    19021 acttcggcgt attctcaacc aagctcttcc ccaacccgcg ccaccattct caccggacaa
    19081 tactccatcc accacggcat tctgatgccg ccaatgtacg ggcaaccggg cgggctgcaa
    19141 gggttaacca cgctgccgca gttgctgcac gatcagggct acgtcactca ggccatcggg
    19201 aaatggcata tgggggaaaa caaagagtcg cagccgcaga acgttggctt tgatgatttc
    19261 cgtggcttta actcggtgtc tgatatgtac accgaatggc gcgacgttca cgtcaatccg
    19321 gaagtggccc tgagtccgga ccgttctgaa tacatcaagc aattaccatt tagcaaagat
    19381 gacgttcatg cggtgcgcgg cggcgaacaa caggccattg ccgacattac gccgaaatat
    19441 atggaagatc tggatcaacg ctggatggaa tatggcgtta agttcctcga caagatggcg
    19501 aagagtgata agcctttctt cctctattac ggcactcgcg gctgtcactt cgataactac
    19561 ccgaacgcca aatatgcggg tagctctccg gcacgcacct cgtatggcga ctgcatggtg
    19621 gaaatgaacg atgtgttcgc taatctgtat aaagcactgg agaaaaacgg tcagcttgat
    19681 aacacgctga ttgtctttac ctccgataac ggaccggaag ccgaagtacc gccgcacgga
    19741 cgcacaccgt tccgtggtgc gaaaggatct acctgggaag gcggcgttcg cgtaccgact
    19801 ttcgtttact ggaaaggcat gatccaaccg cgtaaatctg acggtattgt tgatctggca
    19861 gatctcttcc caacagcact ggatctggcg gggcatcctg gtgcgaaagt ggcgaattta
    19921 gtgccgaaaa ccacctttat cgatggtgtg gaccagacat ctttcttcct gggaacaaat
    19981 ggtcagtcta accgtaaggc cgagcactac ttcctcaacg gtaaactcgc tgctgtgcgt
    20041 atggatgaat tcaagtatca cgtcctgatt cagcaacctt acgcttatac ccagagcgga
    20101 tatcagggtg gattcaccgg cacagtaatg caaacagccg gatcgtcggt gtttaacctc
    20161 tacaccgatc cgcaggagag cgactctatc ggcgtgcgcc atattccgat gggtgtaccg
    20221 ctacagaccg aaatgcacgc gtatatggag atcctgaaaa agtatccacc acgcgcgcag
    20281 attaaatctg attaagccgg cgctatcgct gagatctgcc tttgccggat gcgatgctga
    20341 cgcatcttat ccagcctaca gaacgctgca atttattgaa tttgcacgat catgtaggcc
    20401 ggataaggcg tttacgccgc atccggcaat caaccgcagg cgaccacaaa atggccgctt
    20461 ttttacttac tcaccaccag caaatgcgcc tgcataatgt cgctggccgg gcgaccgtgc
    20521 gccagcaaat cagccattgc tttaagatat ggcggtagat ggcggaaata acgctgatac
    20581 ccggcacaca aataattcag tcccggtttg ccgctggcat cgagcatgaa gcggtgtttc
    20641 gggcagcctc cccaacatgc ttttaacacg ttacaactgc gacactgcgc cggtaattgc
    20701 ttaaatttat cttcaccaaa cacctgctgt tgcggggaat cgatcatttc tgcaattgtt
    20761 tgctggtgca tattccccag tcgatattgc ggataaacat agtgatcgca ggcgtaaacg
    20821 tcgccattgt gctcaacaat caccgagcgc ccacaggttg gctgatgatg gcaaaccgca
    20881 cccggcgcac cgacaaaatt ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg
    20941 acgtcgcgtt taatccagtg gtcgaatatc gccaccagaa attcaccaaa ttcatcgggg
    21001 cgcacagacc attccgtcag ttccccctga atatcaccag gcgcatgtag tttcagtcct
    21061 tcgcgggcag tcgtttcatc agccaggcgc tcgaccaccg gaataaactg gatgaattcg
    21121 actcccgcat cgcacaaaaa atcataaacc tgtaacggtt gctgcgcgct ggtgcggttg
    21181 acgcagacca gcacgttata gtcgacatga tgtttttgca ggagcgtcag ggcacgcatc
    21241 accagtttat gcgtgggtct gccgcctttt gtcacgcgat attgattgtg gatctcagcc
    21301 ggaccatcca gcgataaccc aacaagaaaa tgattttctg ccagaaatgc acaccattca
    21361 tcatcgagca gtacgccgtt agtctggaag ctgttactta tcttcctgcc agcaccatat
    21421 ttcgcctgta gcgccacggc acggcggtaa aactccagcc cgagtagcgt tggttcgccg
    21481 ccctgccagg taaaagccac ttcgttttgc ggttcgctgg cagcgatata gtgacggaca
    21541 tacgcttcca gcgtgtcatc gtccatatgc gtgactggct tttcgcggta aagggattgt
    21601 ttttcgagat aaaaacagta gtcacagttc agattacaat cggaaccgct cggtttcgcc
    21661 atcacatgaa aagcacgcgt tggaacctgt tgcagcatgg tcgctccttg cctgaagtca
    21721 tccttcaata cgttaagggc gaaatcatac cgtggggcaa tgagttatta cgggcgttta
    21781 ttgcgaaggg agggtgacga aatgcacaga aaacaaaaaa ccccgccgaa gcggggttca
    21841 aaattggtcg gcgagagagg attcgaacct ccgacccact ggtcccaaac cagttgcgct
    21901 accaagctgc gctactcgcc gaaatactgc tttttgaatt tttagttcaa ttctttaaag
    21961 tcgtggtgcg agggggggga cttgaacccc cacgtccgta aggacactaa cacctgaagc
    22021 tagcgcgtct accaattccg ccaccttcgc attgtcacaa cttctaataa tggggtggct
    22081 aatgggattc gaacccacga caactggaat cacaatccag ggctctacca actgagctat
    22141 agccaccact acaaatcttg ttacgcggta ttactaccac cgcagctcaa gcgccgggac
    22201 taaatggcgc gcccgacagg attcgaacct gagacctctg cctccggagg gcagcgctct
    22261 atccagctga gctacgggcg cttagcgccg ttgcgggggt ggataatacg gacttccccc
    22321 ccctctgtct agtccctttt taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc
    22381 tgcttattcc tccagtttat gcgctttccc gtggcgatta aggccaaaaa ctttataaat
    22441 cgccgtcacc gccagcataa agatgatgcc aacaaacagc gacatacgcg tatcttcatt
    22501 aaagtacatg ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt tggcccacgg
    22561 gaacaggatt gagcggaacg gatggctggc aatcgccgct ttatgtgcac gccgaaaacg
    22621 cagctggctt atcaatatca caaaccatgg caccatcccc ggaagcacgc tggcactgta
    22681 gacgtagaca aacacacgct gcggattggg aatgatgtag ttcaggcatg agccaattag
    22741 cagaatagca atagatactg ccacacccgc aaccggtacg ccgtgacggg aaactttcgc
    22801 catcgccgcc ggtaactgac ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta
    22861 catgccgctg ttacagccag agagcgcagc cgtcagcacc acaaagttga taatgcccgc
    22921 cgctgcggta ataccgattt tggcaaaagt cagtacgaac gggctgccgt tgctgcctat
    22981 ttcattccac gggaagatgg tgacgataac gaaaatcgcg ccaacgtaga aaatcaggat
    23041 ccgccacagc accttgccta cggcactgcg cagcgtcacc tgcggattct tcgcttcacc
    23101 ggcagtaatg ccaatcagct ccacgccctg gtaggacgcc accacaatac acagcgcggt
    23161 caggaaccct ttccagccac ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc
    23221 aatcgactgc ccgccattgc caaagccaaa gaaaatcacg cccaggccaa tgacaatcat
    23281 cacgataatc gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg
    23341 caccgctgcc agattcgcca acgccaccag cgccactgcg atcaatgcgg gtatccactg
    23401 cgccatctcc gggaaccaga actggacata aacgccaatg gcggtgattt cagagatccc
    23461 caccgccatc cacataaacc agtaagacca ggcggtgaga tagccaaaga acgggctcat
    23521 ataacgatgc gcataaacgg cgaacgaacc ggtaaccggt tcgaggaaca acatttcgcc
    23581 cattgaacgc atgatgaaaa agacgaacag cccggcgatg atataggcca acaatacgga
    23641 tggcccggcc catttcaggg tactggcggc ccccataaac aggccgacgc caatggtgcc
    23701 ccccagggcg atgagttcga tatgtcgagc ttccagccca cgctgtagct ctggtttgtt
    23761 atctgccata aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat
    23821 gggtacatcg agtgttgcga atgttttcgt aattcaggag aaatggcaaa taaagcatta
    23881 aaaatttgaa tgctttgtgt aataaaaaag cagacaggcg acggagtgac cactccgtcg
    23941 ctttacaaag agaggaaaat cagaggttgc cggtgtaatg ccagcgtaaa taacgcagca
    24001 aacgaagctg acgcttaatg cggctcggct gcgaaagcag gcggtagagc cactccagcc
    24061 ccagcgtttg ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc
    24121 cgccaacgcc catatacagc gcatctggat gcaccagacg gcagtcgcgc atgaagatct
    24181 cctgctttgg cgatcccatc gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt
    24241 caaacagcgc ctgacgctgc tcgggtttaa aataaccatc ctgactgcca acgatattca
    24301 cattccactg gttgcgcagt ttagcttcag tttgcgccag cacttcaggt ttaccgccca
    24361 caagaaatac cgtcgtccct tctttgcctg cgcgcgccat cagctcttcc cagagatcgg
    24421 caccggcaac gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc
    24481 tgatgccatc cgcatattta aattcggcag cgttaattaa ctccctgacc tcggcgttct
    24541 cttcaatggt cagcattttt tcagcattaa tggcaaccag cgttccctgc ttaagctgcc
    24601 cgtcagcaaa cagataatcg agggcatgct gcatatcacg ccaaccaatc aactgtaagc
    24661 cacgcagcgt ataggtcggt gccgtggtgt tgttattcat tgttatcctt caacctgcgt
    24721 ccggagcgat gattttgtac gtttatgaat gagtccggcg ctttcaaaaa gccagtacaa
    24781 cagttttgcg atcatcagac atgcgccgaa gaccacgata aaaaagacca cgcgtgagac
    24841 aaacgaatcc agcccttcac gcgccagcac gatcatattg aagatcgccc caaagcagaa
    24901 actgtgcaat atcgcagcct tatagcgatt agtttcgcgg ttgcccagct catacagcca
    24961 gtcgaaccat ttgatgatca gtccaaccac aatcgccccg agcgggatga acaacgcgcc
    25021 gcccatcacc accagtgagc ctataagcgt aggcgagatc gccagtccgg agtggttatt
    25081 cagcacttcc caggtaaagt agttggctga gttcagcacc atactcgggc gacccggcca
    25141 cagccaggaa gggataaaga catagaaatc gcggacaatt ggcgccaggc cctggaagtc
    25201 gatgttgtcg tagttctgca acagcaacgc cagattctcc cacggcgaga aggtgtcgcg
    25261 agtgagatag agaaacgtat agaacgcttc atcgccgctc acattcattc catagcgttt
    25321 tagcgccagc cagaacatgc caacaatccc cagcacgccc gccgccgcca gcatccacaa
    25381 cgaaatccag ccgcgaataa tgccaataaa caggaagata gcgaatgcga tgatgatatt
    25441 ggcgcgagtg ccgccgacaa tcatataagt cagcaagcca aaggcgaccg tgctgacgag
    25501 gaaaaacagc cacgctttgc tgtcctggcg cagaaagtag accaccagca tcgccgggat
    25561 gaaaaagtaa aagaagcgtt ttaacgccac gccggagact tcactggaaa agatctgact
    25621 gtaggagtta agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac
    25681 cagcgcgata cccatcagga tcacccacgt aagattggtt tccacgcggt tcatggtaaa
    25741 cagcggacgg cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt aggtgacgta
    25801 gtaaaccgcg tagaagcagc ccgcagaaag caacgcctgc aacaggattt ctggcggcgc
    25861 gacaccaaca tcaaagcgaa acaccagcac gctggtcagc gggaagccaa agaaaaaggt
    25921 gagcagaaac agcaatgaaa agaatacatt gaagttaaag cgcacacgac gaaactcaaa
    25981 ccaggtcagc gtggcaataa acagcgtgca gagcagccag acaacaaaca ggccactgaa
    26041 ttgcagcaga ctcatgcgac ctccccggcg gcaatcgcca acgcccgctg ccagccttgt
    26101 agatagttag ggctaaagaa ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc
    26161 gcttcacgca caatatcctc gttgagatcg tcggtagtaa acagcaccgg taaatgctgt
    26221 tccgtcatat cctgccagaa cggattttcc cggttaagca cacaaggaat gcccgcctga
    26281 atcagtaagc acagcgtacc aatgccctgc tggcgggcaa aaataaagta accaagatcg
    26341 cactgacgaa gtagcgtcag ataggcgtca aattccagtt tttcactcag aacttgcaga
    26401 ttttcttcgc tgaataactc cagccccgcc tgacgaactt cctcgatgta cgcttcgtta
    26461 tgaggcggat atcccatcgg caccaccact tttaccgtat cgccaaattg ctgatgaacg
    26521 gcgcgcaagg cagcaatatg ctcattgctg cggtcgccgg agttccccac cagaatggtc
    26581 attttccctt cacgttgccg atcgttcgcc atcgtattga gagaagcatc catccgcgtc
    26641 gggaagtaca gcagttcgcc ccgcaccttt gggtgcgttt tggcaaaaaa gctcaaatca
    26701 ccacgggtgg caaatacaca gccgactcgc ttttgcgcca ggcgacgtag tgggtaaaaa
    26761 agcttatatc tcaagccact ggaaagctcg tacaggtctg ccccccagat atgccagtaa
    26821 aactggctgg gcttaatgcc accactcagc agagccagcc acagtgtggg attgaactga
    26881 ccgtggaaga aaaaacgctg ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc
    26941 agcgattttt tcccagggaa aaattgcaca gaaagcgccg gacagctatc acttaagccg
    27001 tcgtccttgc caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca
    27061 ttgaaaaacc gcaaaacggt tcggttatgg tgagggatat ccgatcccag tacgtgaatc
    27121 agtacagtca tgcccgccta cgccagagta aaaacacgcc acaacaaaga gaaaaataga
    27181 cgatataggt tgccatatat gcctgtgccg cgcccagcgc gccgtgcgta gggattagcc
    27241 agtgggcaaa taccatcaat aaagtgaact ggctgacttc cgccagaata taaaaccgca
    27301 gtgaagcttt ggcgatcacc agataaccaa agacataagc gcccactttt aacacatcac
    27361 ccaccaactg ccaggcaaag agatcgcgca tagcggtaaa tttattcgac aacagcagcc
    27421 agatagcaaa atcacgcagc aaccagacgg taaaactcgc cgccgccact gccggtaaga
    27481 cgaatttcag cgatttaacc acttcccggg tgatatcgcg cttttccgtt agccgcgaca
    27541 acgtgggcag caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa
    27601 tactgctcac cccttgccag atccccacct catcccagct atactgcgcc gccagcagtt
    27661 tacgcatcat gatgtaagca acaggcaagg tcaccgacgt aatcaacgtc atgagcgtaa
    27721 atttgctcaa ctgccctgcc agaccgttat cccagctggg tttcagatag cttaacggga
    27781 tagcaccacg cttaattaac atgacggcgg caggaatcac caccagcgcg ggaatcaacg
    27841 ccagacccag caacgcccct tcatatccgc ccaaacggta actgacgtag tacgcaagaa
    27901 cgccaatcaa gctgccaaca atcagagata acgcattacc tgcggcatcg cgaaagcctt
    27961 tcatcaacgc cagtaacagg ttgccccagg cgatccccat ttgcaccagc gccactaaac
    28021 gcaccagccc ctgatagtcg tgattaccaa acaatcccaa gctgattggc gcagctgcca
    28081 gcacaaaaac cagcgccatc agcgtagaga agccaagtac catcgctgat gaagtgccga
    28141 ccacgcggcg cagctgttgc ggattatcat ggtactgggc aacgtatttg gttacaccgt
    28201 taaagatgcc agccccggca agcacgccga gcacggtaat caactggcgg aaatttgccg
    28261 ccagcccaag ccccgccgga ccaaatgaca ccgccagcaa cttaccgacc agtaacccgg
    28321 caccaatctt gaccagtgta ctggccgccg tccacaagga cgcttttgcc aacgacatat
    28381 caggagaagt agttcaacaa agtcgcaatt accgtacgct gattgacggg cgacaggttg
    28441 tagaacagcg gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg atcttcaccg
    28501 tggaactcac caaagcgttc ccccgcaggg caaccgtgca gcggaatgta atggaacacc
    28561 gccatgattt ccgcttcttt cagaaagtta atcaacgcgc tccggtcacc aatatcccgc
    28621 agtttaatgt aaaacatatg cgcgttctgc aagcagccat cgggaatcga cggcagctcg
    28681 atacgcccgg ctttcgccag aggtgctaac gcatcgtagt agttttgcca cagcgccaga
    28741 cgttgctggt tgatacgatc cgctgcttcc agttgcgccc acaggtacgc agcttgcaga
    28801 tcggacatca aatagctgga gccgatatcg cgccaggtat atttatcgac ctgaccacgg
    28861 aagaactggc tgcggtttgt gcctttttca cggatgatct cggctcgttc gatcaacgct
    28921 ttatcgttaa tcagcgtcgc gccgccttca ccgcccgccg tgtagttttt ggtttcatgg
    28981 aagctaaagc agccaatatg accaatggtt cccagtgcac gccctttgta agtggacatc
    29041 acgccctgag cagcatcttc caccacaaac agattatgct ttttcgccaa cgccataatg
    29101 gtgtccattt cgcaggccac gcccgcgtaa tgcaccggca cgataacgcg cgttttgtcg
    29161 gtgatcgccg cttcaatcaa cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca
    29221 aaaacgattt ttgcgccacg cagcacaaag gcattggcgg tggagacaaa ggtgtagctc
    29281 ggcatgatca cttcatcgcc aggctggata tcgagcagca gcgccgccat ctccagcgaa
    29341 gcggtgcagg acggcgtcag taacactttg gcgctgccaa aacgttgctc cagccactgc
    29401 tggcagcgac gggtaaaacc gccatcgcca cacagtttgc cgctacccat tgccgactgc
    29461 atatagtcga gttcggttcc caccaccggc ggtgcgttaa atggaatcat gtgatcacct
    29521 gtataaccag tacgcggtgc tttctacatt cgcaccactt tgtatgtatc gtttaagcgc
    29581 ggcggtgttg cccatttggg tcgccacccg caaagttgtt ttaccgcgag catacgccca
    29641 gtttagcgcc gtttgcatca gctcagcacc tgcaccgcgt ccagccagca ggccaattcg
    29701 cgcatctgta gcattgagtt cccgtaaaga gacataaccg cgaatatcgc cggacgccgc
    29761 acgtaaaatc agacattgat gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc
    29821 ataaaagcga ctgctggcgt caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa
    29881 tgcggcgctg gctaactgac gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc
    29941 gctatcactg gcattgttca cgggtagcgc caaatcaact tcaccttcta ccagggagaa
    30001 tcccagctgt tgcagggcat ccagttcccc cgtatttgat gccgcaattt tggcctgcac
    30061 ccgtgaccac ggcgctaacg cgtctggcgt caggaacggt gcttcagacg taatgcgcac
    30121 gatggcgctg ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg
    30181 gacgggcacg aagtaactcc agcagatatt ggccgtagcc agttttcgct aatgaactgg
    30241 cagcacgctt cacaccctcg tcatcgagcc agccgttacg ccaggcgatc tcttccaggc
    30301 aggcaatctt aaagccctgg cgtttttcca ccgtctgtac aaaggtgctg gcttcaatca
    30361 ggctgtcgtg agtgccagtg tccagccagg caaatccgcg cccgagcagt tcaacggtca
    30421 ggttgcccgc ctcgaggtac atctggttga tggaggtaat ctccagttca ccacgctccg
    30481 acggcttcac ctgctttgcg tactccacga ctttactgtc gtagaaataa agcccggtca
    30541 ccgcccagtt tgatttcggc tgttttggct tttcttccag cgagatagcg cggaaattat
    30601 cgtcaaactc caccacgcca aagcgttccg ggtccatcac ctgatagcca aaaaccgtcg
    30661 ccacttccgt gcgcgccgca acatgacgca gcttcggact gaagccctga ccgaagaaga
    30721 tgttatcgcc cagcaccaga caagaaggtt caccattaag gaaggtttca ccgatgataa
    30781 aggcctgcgc cagaccgtcc gggctgggct gttcggcata ttccagctgg ataccgaact
    30841 cactaccatc gcccagcagg cgctggaaat aacctttatc ttccggcgta gtgatgatga
    30901 gaatttcgcg gataccggcc agcatcagca ccgacagcgg atagtaaatc attggcttat
    30961 cgtaaatcgg caacagttgc ttcgatacgc cgcgcgtaat cggatgcaat cgggtaccgg
    31021 aaccgcccgc caggataata cctttcatgt ttgcctccgc tgaaaattag cctttcagac
    31081 ctaaacgctc gccctgatag ctgccgtcct gcacctgctt cctccagctt tcattagcca
    31141 gataccactg caccgtttta cgcattccac tttcaaaggt ttcctgcggc agccagccaa
    31201 gttcacgggc aattttcgaa gcatcaatgg catagcgcag atcatgcccc ggacggtcag
    31261 cgacaaaggt gatcaagtca cgataatgca ccacgccttg cggcttgttc ggagccagtt
    31321 cttccagcag ctcgcaaatg gtttccacaa catcgagatt cttacgctcg ttgtgaccac
    31381 caatattata ggtttcaccg actttcccgg tggtcgccac gcaatacagc gcgcgggcgt
    31441 gatcttcaac atacagccag tcacggattt gctgcccgtt gccatatacc ggtagcggtt
    31501 tacccgccag cgcgttgagg atcatcagcg ggatcagttt ttccggaaag tggtaaggac
    31561 cgtagttatt cgagcagttg gtgataagcg ttggcagacc ataggttcgc agccaggcgc
    31621 gcaccagatg gtcgctgctg gcttttgacg cggaataagg gctgctcggc gcatacggcg
    31681 tggtttcggt gaagaaatca tccgtcgagt gcaggtcacc atatacttcg tcagtggaga
    31741 tatgatgaaa acggaacgct gattttttat cttccgtcag cgcattccag taagcccgcg
    31801 ccgcttcaag caatgtataa gtcccgacaa tgttggtttc aataaacgct gccgggccgt
    31861 caatagaacg gtcaacatgg ctttcggctg ccagatgcat gacacagtct ggctgatgct
    31921 cagtgaatac gcgcgccagt tctgcccgat cgcagatatc aactttctca aaggcaaagc
    31981 gctcgctttg cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga
    32041 ccactaccac cgcgtcgctc gtttcgttga tgatataacg caccagcgcc gagccaataa
    32101 acccggcacc gcctgttatc agaatctttc tcatcagcgc cagactcctt tagcatcgac
    32161 gacatactgc tgatggacat tgtcgccatt gataactttg aactgactat gatcgaccag
    32221 catcaccagc acgtctgccg ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc
    32281 ggtcagtttt ttcggcaact ggtggatgtt aggctcaaca accagagttt cgccgctatg
    32341 ccactgggcg atcagttcag cgatttccat cgccgggctt tcgcgcaggt catcaatatt
    32401 cggtttaaac gccagaccaa agcaggcgat tttcagttca ctggcgcgtt tatcggtagc
    32461 cgccaggcaa tcagccaccg ccgctttcac ctgatcgata acccagaacg gtttgtgatc
    32521 gttcacttcg cgcgcggtac ggataagccg cgcctgctgg gggttctgtg ccacgataaa
    32581 ccacggatca acagcaatgc agtgaccgcc cacgccaggg ccaggctgaa gaatattaac
    32641 gcgagggtga cgattcgcca ggcgaatcag ttcccagacg ttaatcccct gatcggcaca
    32701 aatcagcgac aattcattag caaaagcgat attcacatcg cggaagctgt tttcggtgag
    32761 cttacacatt tccgccgtcc gcgagttagt gacgacacac tcaccttcga ggaaaatttt
    32821 gtacagttcg ctggcgcggg ccgaacaaac cggcgtcata ccaccaatca cgcgatcgtt
    32881 tttaatcagc tcgaccataa cctggcccgg taacacgcgt tccgggcagt aagcaatgtt
    32941 gacgtccgcc tgctcgccca cctgctgtgg gaaagtgaga tccggacgca tctctgctaa
    33001 ccattctgcc atcttctcgg ttgaccccac cggcgaggtg gattcaagga tcaccagcgc
    33061 gccttttttc agcactggcg caatggagcg agcagccgat tcaacgtagg tcatatctgg
    33121 ctcatgatcg cctttaaacg gcgtgggtac agcaatcagc caggcatccg cttcaactgg
    33181 cgtcgtgctc gctcgtaaaa aaccgccttc tacggcagtt tttactacgc tcgccaaatc
    33241 aggctcgacg atatggattt cgccacgatt gatggtatca accgcgtgtt ggttgatatc
    33301 gacaccaatc acctgttttt gccgtgaggc aaacgctgct gctgtaggca gcccgatgta
    33361 acccagtccg ataacagaaa tggtcgcaaa actcatagtg atatccgatt attttttaac
    33421 gcttccagaa tgcgagagca tgcctgacca tcaccatacg ggttatgggc gcggctcata
    33481 gtttgatatt cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta
    33541 tccgtgccta ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg
    33601 cgcatcacca gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg
    33661 gtcaaaatca gccaggcgtg gttcatcagc cagacaaacg gtaaatactc ctgaggatcg
    33721 atcagaatga catttttcac atgccccaga atgcgattga ccggctctct gacgttcggg
    33781 ttgagatgca ccggatagac aatctggatg tcctggtgcg tggtggcgat gtctgccagc
    33841 gcgtggcaga tttcttcaaa gccacgaccg aagctttcac gcctgtgacc ggtcaccaga
    33901 atcatctttt tatcggggtc gataaacggg taatttgccg caagttctga acgcagcgtg
    33961 tcgctgctca tcacctggtc acgcacccat aacagtgcat caatgactgt attaccggta
    34021 atgaagattc ggctatctgc aacgttttca cgcagcaagt tttgccggga agtttcggtt
    34081 ggagagaaat gatacatcgc cagatgcccg gtcaatgtac ggttagcctc ttccggccac
    34141 ggcgaataga gatcgcccgt gcgtaaacca gcctcaacgt gaccaacagg aatacgctga
    34201 taaaacgccg ccaggctggt tgccagcgtc gtcgttgtgt cgccgtgaac cagcacgacg
    34261 tctggtttga actcggcaag aataggtttt agcccttcca gaatccgaca ggttatctct
    34321 gtcaggccct gtcctggctg cattatgttg agatcgtagt caggtacaat ggaaaagagt
    34381 ttcagcacct gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa
    34441 aaaggatctt ttgccaacgc atgcaccaac ggagccatct tgatggcttc cgggcgcgta
    34501 ccaaatacag tcagtacttt cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg
    34561 ctcaccgcag cagtgttgct atttcgagca acggcgggtt aatgcgacac cagccccgat
    34621 cagccccccg acaatgcccc acataatcat caggaaggca cgacgtgggc tatcgcgttt
    34681 taccggttct tccggcgtac gcaaatagcg ataggtctga aaacgcggat ccagggttgg
    34741 accaacattc agggtgttta acatggcccg attctgatca tagtcgagat caaaggccgg
    34801 accgacggcc tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat
    34861 ttctgaatca ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg
    34921 ctcagcaatt ttcagcgcct gctcaatgct gttcatccgg cggtcgtaga tggctttcgc
    34981 cacctcttcc tgacgcttca cctgtgcttt catctggatg gtacgcgccg cccatgcgcc
    35041 tttcagctca tcattcagat ggctggctgc acgctggctg gcaaaagcaa catactgacg
    35101 taacaggtta ttagcgtcag gcgcggtttc ggcaataagc ttcacgctgt cattgaccgc
    35161 gcgggtaaag tctccgggga taaactggat gttgttaatc atttcatcca gcaacgccgc
    35221 atcggctttg ctgttgccca ccatccgctg cttgtaatag tcggtttgca gccagaactc
    35281 tctgcgggta tcccacgagg ccagttgcat aacaaactct ttataggctt cgtccatgac
    35341 cgatggttgg tcggcagaag ccatgtttga acggacatcc aggttacgca aaaattgctg
    35401 ctgcgagtaa tatcccccca gcatattcac cgttggacga tcggtaatcg ccgtcgagct
    35461 ccactcctga cgagcaaaaa acgtatacgc cagcgcgatt aacgcaaacg ccagccccat
    35521 gccaataatc catagcttcc cagcccacaa ggtacgaaac aacccacgaa tatccagttc
    35581 attttcagcg tcttcggccg gtttcccagg cattggttgt gtcatcacat cctcatttat
    35641 ttggttaaat tggggctgcc accacgattt ctacgcagtc tgcgttttac gcgcttaata
    35701 aagcgagcaa ctttccaggc acgcttaatg caatatccat agaggaggaa tgctagcaaa
    35761 aagagcacca acatgaccca ctccgggaca aaatgagaat attctgccag cacgccaatg
    35821 gaagcgagca gtgctgcggc aagggtaatc agcacaaacg cctggcggga agtaaacccg
    35881 gcacgcatga tcaaatggtg aatatgctga cggtcaggag agaatgggct catgccttta
    35941 cgcaggcgac ggtacataat cgccaccata tccattagcg gaatggcgat tatccacaaa
    36001 gcggtaaccg ggctgatggg atgggttttg ccttgggtcg tttcgagcag gatccagatc
    36061 acggtaaaac caatcagcgt actgcccgca tcacccataa agactttgta gcggcgaccc
    36121 aggataccaa ggttaagcat gatatatggc aggatggcgg cgatcatcgc aaagcaccag
    36181 attgcgaggc tggtttgccc gtcgaaccac aaaatcatac cgattgctgc aaacgagacg
    36241 caggacaacc cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc attaatggcc
    36301 gcccagacgg caaatagcgt caggaagtaa ccaaacggtc cgagcaccat ctcccaggag
    36361 ccaaagatat aacccaggct actgagataa aggttgccaa acaccatcat aacaatgcca
    36421 acagcggcct gtatggtggc acggattttt acgctgatat caaaacggtc atccagcgcg
    36481 ccaatgaaaa caagcacacc ggcacaagcg agatagagag atgcatgcgg aatatagtaa
    36541 tcgacaattc cgaacgtgaa gcaaatccct gcgtaaaccg aaataccccc aacgagtggt
    36601 atcaacccct ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac tttttttgcc
    36661 accttacggg caaaaaacag aaacagtgtc gtgaataaaa aaatactgat gagatcagta
    36721 ctcactgtca gtaaattcac aatgcatgct ctcagagaaa attattagca gaagtataac
    36781 cacgaagacc tttattcaga agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa
    36841 ttaaccattt gatatgcaaa ttgcataaca tgccagcaaa ttccaggaat tttcctaaca
    36901 tcgccagcgc ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg
    36961 tttttggcat aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg tcattggtct
    37021 tggtcattgc cagtttatta atgaggaatt ccattgcatc gatttcgccc atcgggtgaa
    37081 tgattttgcg caggatccac attttctgca gttcttcctg agtcgtgagc agctcttctt
    37141 tacgggtacc ggaacggttg tagtcgatag ccgggaagac gcgtttttca gcgatcttac
    37201 gagagaggtg cagttccatg ttgcctgtac ctttaaactc ttcgtaaata acttcgtcca
    37261 ttttagaacc ggtatcgata agcgccgtcg cgataatggt caggctgccg ccctcttcca
    37321 cgttacgcgc cgcaccgaag aagcgtttcg gacgatgcag ggcgttggca tccacaccac
    37381 cggtcaacac tttacctgac gccggaacaa cggtgttgta agcgcgcgcc agacgagtga
    37441 tggagtcgag cagaatgata acgtctttct tgtgctcaac caggcgtttc gccttctcga
    37501 tcaccatttc cgcaacctga acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa
    37561 cttcaccttt taccagtcgc tgcatctcgg ttacttcttc cggacgttcg tcgatcagca
    37621 gaaccatcag cacacaatcc gggtggttgt aagcaatgct ctgagcaatg ttctgcagca
    37681 gcatggtttt accggctttc ggcggtgcca caatcagacc acgctgacca cgaccgatag
    37741 gtgatgccag atccagtacg cgagcggtta aatcttcagt agaaccgtta ccacgttcca
    37801 tacgcagacg agagtttgcg tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg
    37861 cgttttcagg tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa tagcgttcac
    37921 cttctttcgg cgggcgaatc ttaccagaga tggtatcacc agtgcggagg ttgaaacggc
    37981 ggatttggct aggggaaacg tagatgtcat caggaccggc gaggtaggag ctgtctgcgg
    38041 aacggaggaa accaaatcca tcctgcaata tctccagtac gccatcacca aagatatctt
    38101 cgccactctt tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta cgcatacgag
    38161 ccaggttttc cagccccata ttttcgccga gagtgatcag ctcagaaacc ggcgtattct
    38221 ttaattcggt aagattcata gtggtgtgag ttcttaaact tggggtaaat ctcgaactta
    38281 atgttgtgaa tggtatggca ggatcatcca tgcctgttta cggtcatcga ctcatgtctt
    38341 ttcgctgcct ggtcacagga gagtacgcag aactgaaacg acaagacgga ttgagtgacg
    38401 agccagaaat ctgttcactt cgcatttaag ataaaacggg aagcgttgga aattacaaga
    38461 ttcaaactta ataaggtatg tttaatacga agtcaacact aagttagcat gactcacgcc
    38521 gggcgtccag tttttagcga cggggcaccc gaacatgaaa ttcccttacg ccaggttagc
    38581 gtcgaggaac tctttcaact gacctttaga cagtgcgccc actttggttg ccgccacttc
    38641 accgtttttg aacagcagca gagtcgggat accacggatg ccatatttcg gcgcagtgcc
    38701 agggttttga tcgatgttca gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat
    38761 ttcatccaga atcggggcga tcattttgca cggaccgcac cactctgccc agaaatcgac
    38821 gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat
    38881 aattttatcg ctcatatata actccacagg aataagcctg gcgtgttggt ttcgttgttg
    38941 gtgtaacatt aaccaactaa aggttgactt tatttcaccg gatacgcttt cgtaaagcaa
    39001 tagtaagctg atattctacc acactatgag caaaacacat ttaacagaac agaagttttc
    39061 cgacttcgcc ctgcatccga aggttgtaga agcccttgaa aaaaaagggt ttcataactg
    39121 tacgcccatt caggcactgg cccttccgct gacgctggcg ggtcgtgacg tagccgggca
    39181 ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg tcaacgtttc attgtcttct
    39241 ctctcatccc gcgattgccg atcgcaaggt gaatcagccg cgtgccttaa ttatggcacc
    39301 gacgcgtgaa cttgccgtgc agatccatgc cgatgcggaa ccgctggcgg aagctaccgg
    39361 cctgaagctg ggtctggctt acggtggtga tggttacgac aaacagctga aagtgctgga
    39421 aagcggcgtt gacattctga ttggcaccac ggggcgttta attgactacg ccaagcagaa
    39481 ccacattaac ctcggtgcca ttcaggtagt ggtactggac gaagccgatc gcatgtacga
    39541 tctgggcttt attaaagata tccgctggct gttccgccgt atgccgcctg caaaccagcg
    39601 cctcaacatg ctgttctccg ccacgctttc gtaccgggta cgtgaactgg cgttcgagca
    39661 gatgaacaat gccgaatata ttgaagtgga accggaacag aaaacgggcc accgtattaa
    39721 agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga
    39781 agaagagtgg ccagacagag cgattatttt cgccaatacc aaacaccgtt gtgaagagat
    39841 ctggggccac ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca
    39901 gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggc gatctggata ttctggttgc
    39961 caccgacgtt gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct ttaactacga
    40021 tttacccgat gactgtgaag attacgttca ccgtattggt cgtacaggtc gcgcaggcgc
    40081 aagcggtcac tctattagcc tggcgtgtga agagtatgca ttgaatttgc ctgctattga
    40141 gacctatatt ggtcactcaa ttccggtaag caaatacaat ccggacgcat tgatgaccga
    40201 tctgccaaaa ccgctgcgcc tcacgcgccc gcgcacaggc aatggtccgc gtcgtactgg
    40261 cgctccgcgt aatcgtcgtc gttcaggtta aataaaaaat gtcatactca aaatcattca
    40321 aactgtatca agatggcaag agaatgaatc ccgatgagct tactgtagta agtgattcag
    40381 gtgaaagaat gcagccaaca cagagacaga ttgaaggatg aagagtatgg gttccacctc
    40441 atcgctgtat gcagccattg atctcggttc gaatagtttt catatgctgg ttgtgcgcga
    40501 ggtggctgga agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc tggctgctgg
    40561 cctgaatagc gaaaatgccc tctccaatga agcaatggag cgcggttggc aatgtttgcg
    40621 cctgtttgct gaacgtctgc aagatattcc tccctcgcaa attcgcgttg tcgctacggc
    40681 gacgttacgc cttgccgtca atgcgggtga ttttattgcc aaagcgcagg aaatcctcgg
    40741 ttgtccggta caggtgatca gcggtgaaga ggaagcacgt ctgatttatc agggtgttgc
    40801 tcacaccact ggcggtgccg atcagcgcct ggtagtggat atcggcggtg ccagtactga
    40861 actggtaacc ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt
    40921 cacctggctg gaacgctatt ttgccgatcg taatctgggg caggaaaatt ttgatgctgc
    40981 agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat gaattacggt atcacggctg
    41041 gaaagtgtgc gttggtgctt ccggcaccgt gcaggcgtta caggaaatca tgatggcaca
    41101 ggggatggat gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc gagccattca
    41161 ttgcggtcgg ctggaggaac tggagattga cgggctgacg ctggaacgtg cgttagtgtt
    41221 cccgagtggt ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc agtgtatgac
    41281 cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc tggccgtcga
    41341 gcaggatatt cgcagccgta cgctgcgtaa tattcaacgc cgctttatga tcgatattga
    41401 tcaggcacag cgcgtagcca aagttgcggc taacttcttc gatcaggtgg aaaacgaatg
    41461 gcatcttgaa gcaataagcc gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg
    41521 cctgagcgtt gacttcaaac aagcgccgca acacgctgct tatctggtgc gtaatctgga
    41581 tcttcccggt tttacccccg cacagaaaaa attgctggcg acgctactgc tcaaccagac
    41641 taatccggtc gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc gcgtcgcaga
    41701 acaactctgc cgtttactac gcctggccat catttttgcc agccgtcgcc gtgacgatct
    41761 cgtgccagag atgacattac aggctaacca tgaactgttg accttgacgc ttccgcaagg
    41821 ttggctaacc caacatccgc tgggtaaaga gattattgct caggaaagcc agtggcagag
    41881 ctatgtccac tggccgctgg aagtgcatta atgagtaagt gccggatgcg atgctgacgc
    41941 atcttttccg gccttgatta tttccctcgt tttgccgcca tcatcgcttt cagattcgcc
    42001 agatggcttt gccctttctg catccgttct tcggcgctga ccactttgcg ctcctgttcc
    42061 caaatcagat catcctgcgg cagctccagc aaaaagcggc tcggctccgg gcgcaccagt
    42121 tcgccgtact gacggcgttc tttacacagc gtaaaggtca attccttctg ggcgcgggta
    42181 atgccgacat aggccagccg ccgctcctca tcgatattat cttcatcgat actgctctgg
    42241 tgcggcaaaa acccttcttc cataccgacc atgtagacat aaggaaactc cagtcctttt
    42301 gacgcgtgga gagtcatcag ttgcacctga tccagctctt cttcactctc accacgctcc
    42361 atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt
    42421 tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc
    42481 atttcggcgg ctttcgggct gggcgatgtt tcgtacagcc aggattcata atccatgcca
    42541 tggatcagat cacgcaccgc agcaatcggc tcccgctccg ccaggcgctg gatttctgcc
    42601 aaccagtgag tgaagcgggt caatgcttca taaccacgtc cgctaagcgt ctgactcagg
    42661 cccatatcaa agctggcggt aaacatgctt ttattgcgcg tcatcgccca ctcacccagc
    42721 tttttcagcg tagccgggcc aatctctcgc ttcggcgtgt taacgatacg cagaaatgcg
    42781 ctgtcatcgt ccgggttagt cagcacgcgc agataagcca gcaagtcctt gatttcagga
    42841 cgagagaaaa acgacgtacc accggatatt ttgtacggga tgcggttttg catcaggaat
    42901 ttttcaaata cccgcgactg atggttgccg cgataaagaa tggcgtaatc tttgtactgc
    42961 gttttattga cgaagtgatg ggcgatcagc tcgccagtaa cgcgctcggc ttcatgttcc
    43021 tcgttattcg cgcttaaaac ttttagctct gtgccgtaac ccagttcgga gaacagacgc
    43081 ttttcaaaga cgtgcgggtt attggcgatc aggatgttcg ccgctttcag aatacgcccg
    43141 gaagagcgat agttctgctc aagcttaatc accttcagcg ccggaaaatc ctgactcagc
    43201 agcaccaggt tttgcggacg tgcaccgcgc caggagtaga tcgactggtc atcgtcaccc
    43261 accacggtaa agcgcgcgcg gctgcccacc agcagtttca ccagttcata ctggctggtg
    43321 ttggtgtcct gatactcatc caccagcagg tagcgaattt tgttctgcca gcgttcgcgt
    43381 acttcttcat tgcgttgcag cagcaacgtc ggcaataaaa tcaggtcatc gaagtcgaga
    43441 acgttacagg ctttcaggtg tgcatcatac agcccataac aatgggcaaa aatacgatcc
    43501 cgctcgccct ttgcctctgc cgccgcctgc gccggagttt tgagatcgtt cttccagtta
    43561 gagatggtcg aaatcagttg ttgcaggaga actttgtcat cttcaatcag cccctcggtc
    43621 aactctttaa gcaaagcaag ctgatcggta tcgtcaaata acgagaagtt cgctttcatc
    43681 ccaagcgccg catactcgcg tttgatgata tccagcccca acgtatggaa agtggagatc
    43741 atcagcccac gcgcctcttt acgccccagc gtctgcccta cacgctcttt catctcgcgc
    43801 gctgctttat tagtaaaggt caccgccgca atgtgccgcg cctgataacc gcaaccgcgg
    43861 atcagatggg cgattttatt ggtgataaca cgagttttac cggaacccgc gcccgccagc
    43921 accaggcagg ggccggtaac gaattcgaca gcttgttgtt ggccggggtt tagacgcata
    43981 ggtgtattgc tcaatcttcg aacgggggga ggattgtagc agaatcgcat ttgaatgtga
    44041 ccgcgtgtaa gccgctaacc ggttggctgc ggatgtttaa atcagagaga gaagaccgaa
    44101 atcggagttc atcgtgaatc cattagactt agaaaatatc gggtcgtaac gttgtgttat
    44161 tactttttca gagtttacta aggagaggtt tactcgtggc gattaatttc agccctaagg
    44221 ttggtgaaat actggaatgc aactttggta actatcctgt tagccaaaat ggaccgttca
    44281 gcacgacgta ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg ttagttgtcg
    44341 tgctcaacgg aaaaattaat ggcaacgcat tcatcgtcgt tccattatca accactcgcg
    44401 accatgacaa gttaaaacgt ggaatgcatg ttgaaatagc cagtaatgtt atcaatgacc
    44461 tgcaattctt tgatcagcag attcgttggg ccaaaaccga cctggtacaa caagtcagtc
    44521 gaaacagatt aaacagagca agaacttaca gagggtatct taatcaatgt ttgccacatg
    44581 agttggttgc agacatacag agggcggtca tcaagtcaat caatgccata tcgctcataa
    44641 attgacaata aaatcaccat ccccgataat gactttgtag cccatagagg ccctaagttt
    44701 tctccccttg agggacctgc gaagcccgga ttttccgggc ttttgccata tatgtaccag
    44761 cccctccctg actgacaatt acgttagcat ctgcggatca tgctaaaatc gccgcctgac
    44821 aattattcac tcaaggcacg atcatggcaa aaacagcagc agcactgcat atccttgtaa
    44881 aagaagagaa actggctctg gatcttctcg agcagattaa gaacggggcc gattttggca
    44941 agctggcgaa gaaacactcc atttgcccat caggcaaacg cggcggtgat ttaggtgaat
    45001 tccgccaggg tcagatggtt ccggcgttcg ataaagttgt gttctcttgt ccggtactgg
    45061 agccaaccgg cccgctgcac acccagttcg gttaccacat cattaaggtg ctgtaccgta
    45121 actaatagca aggccttctc cgggagaagg ccttgaactc tgaatcgcta ttaacccgca
    45181 acagcaatac gtttcatatc agtcatgtag ccgcgcagtt tcttacctac ctgctcaatc
    45241 gcatggctgc gaatcgcttc gttcaaatca cgcagttgcg cgttatctac cgcaccttcc
    45301 ggaatagctt tacccaaatc gcccggttgc agctctgcca taaacggttt cagcaacggc
    45361 acacaagcgt aagagaacag atagttaccg tactcagcgg tatcagagat aaccacgttc
    45421 atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagttc gtgcagtgat
    45481 tcatagtaag cagattcttc gatgatgccg gaatcaacca tggtttcaaa cgccagctca
    45541 acgcccgctt tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg
    45601 attttgcctt catactgcgg cgcggtttca aacgcggttt tgccggtctc ttcacgccag
    45661 gtcagcagtt tcttatcatc gttggcccag tccgccatca tgccggagga gaattcgccg
    45721 gagatgatgt cgtccatatg tttctggaac agcggtgcca tgatctcttt cagctgttct
    45781 gacaacgcgt aagcgcgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg
    45841 ccgccctgtt tcagcgcttc ggtgatggtt tcccagccga actgaatcag tttttctgcg
    45901 tatgccggat cggtgccttc ttccaccagc ttgtcgaagc acagcagaga gccggcctgc
    45961 aacataccgc acagaatggt ttgctcgccc atcaggtcag atttcacttc cgcaacgaaa
    46021 gaggattcca gcacgcccgc acgatgacca ccggttgccg ccgcccatgc tttagcgatc
    46081 gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg
    46141 aatccacgtt tgtactcttc acgtacttcg gtgcctgggc atttcggcgc aaccattact
    46201 acggtgatgt ctttacggat ctgctcgccc acttcgacga tgttgaaacc atgcgagtaa
    46261 cccagcgccg cgccgtcttt catcagtggc tgtacggtgt gcactacatc agagtgctgc
    46321 ttgtccggcg tcaggttaac caccagatcc gcctgtggga tcagttcttc gtaagtaccc
    46381 actttaaaac cattttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct
    46441 ttacgcagag cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcaga
    46501 ccctgtgcgc cacagccgac gatgaccact tttttaccct gaaggtagct cgcgccatcg
    46561 gcgaattcgt cgcggcccat aaagcgacat ttgcccaact gtgccagttg ctggcgcagg
    46621 ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt
    46681 cggttcgcgt ttgcaagatt gaattcacta tatgacagga aatttattgc ggaaattgat
    46741 atattcacaa catcatattg caatttttgc aacgtcaaca tcgagggctg tccctgtgga
    46801 tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc
    46861 gcgggcgatg cacgttagcc catccacgct ctcacggcag attcagcgcc tggaagaaga
    46921 tctcggtcag ccgctgtttg tgcgcgataa ccgcacggtg acgctgactg aagcgggcga
    46981 agaactgcgc gttttcgccc agcaaacgct gttgcagtat cagcagctgc gccacaccat
    47041 cgatcagcaa gggccgtcgc tctctggcga attacatatc ttctgctcgg tgaccgctgc
    47101 ctacagccat ctgccgccga ttctggatcg cttccgcgcg gaacacccgt cggtggagat
    47161 taaactcact actggtgatg cggcagatgc aatggaaaaa gtggtcacgg gagaagcgga
    47221 tctggcgatt gccgggaaac cggagacgct acccggcgct gtagcgtttt cgatgctgga
    47281 gaatctggcg gtagtgctga ttgcgcccgc gctgccctgc ccggtgcgta atcaggtctc
    47341 ggtagagaag ccggactggt caacggtacc gtttatcatg gccgatcagg ggccagtacg
    47401 ccgccgcatt gaactgtggt ttcgacgcaa taaaatcagc aacccaatga tttacgccac
    47461 ggttggcggg catgaagcga tggtatcgat ggtggcgctc ggctgtggtg tggcgctgtt
    47521 gccggaagtg gtgctggaaa acagcccgga gcctgtacgc aaccgggtaa tgattttaga
    47581 tcgcagcgat gagaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa agcggctgca
    47641 tgagccgctg attgaggcgt tctggaggat tttgccgaac cacaaatgac gttgtcgcgc
    47701 gagtaggcct gataagcgaa gcgctatcag gcatttttcc ctaacccgcc aaaaagaacc
    47761 tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagctcattc agccgggttt
    47821 cgaaatccgg ttcatggtcg ccaagttcga acgccgccag tacgcgcccg tagtcggtgc
    47881 catggctgcg atagtggaac aaagaaatgt tccagtgcgt acccagcgtg ttgaggaagc
    47941 gcagcagcgc gcccggtgat tccgggaatt cgaagctgta gaggcgttcc tgcaacggat
    48001 gtgacggacg cccgccgacc atatagcgca catgcaactt cgccatttcg tcgtcggaga
    48061 gatccaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga
    48121 ggccgcggct caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg gcaaaacggt
    48181 agttgaactc ggtgaccgat cgcccgccaa gcagttggca gaatttgagg aagctgcctt
    48241 tttcttccgg aatggtcacc gccaacaacg cttcacgctg ttcgcccagt tcgcagcgtt
    48301 ccgagacgta gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc
    48361 gttcgccgcg aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc
    48421 cagagggttc cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc gcacagatcg
    48481 catcactatc gacggtgatg atatcgtcga gatactcctg gcacaaacgg aaggtttcgt
    48541 cgccgatgcg ttttaccgca acgccttcag caaacagccc cacgcgcggc agatcaaccg
    48601 gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatta
    48661 ctttgatttg cggcatcagt tgtttgatca gcaccgccac gccagcagcc agaccgccgc
    48721 cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca
    48781 gcgtgccttg cccggcaatc accatcggat ggtcgaacgg cggcacccag gtgaacccct
    48841 gctgctgtga cagttcgatc gctttggctt tcgcttcatc aaagttcgcg ccgtggagca
    48901 gcacttcgcc gccgaagccg cgcaccgcgt cgactttgat gtcggcggtg gcggttggca
    48961 taacgatcag ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt
    49021 tacccgcaga agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca
    49081 tggcgtatgc gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca
    49141 gaatcacgtt atcaagacga gacgagagct tttccatttt ttgtagcggc gtgacctgcg
    49201 ccgcttcgta gaccggcgcg cgcagcaccg ctcttaaata ttcagcacct tccggggcac
    49261 cggacagggg ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc
    49321 gcctttgtcg gcgctggttg ccaggctggc ataagcacgc agggcaaagg agatctgacg
    49381 ttcacgattt ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc
    49441 cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcggaatgt cgatggcgat
    49501 caggtcgccg tcttcaatca ggccaatgct gccgccgctt gccgcttccg gtgagacgtg
    49561 gccgatagaa aggccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc
    49621 tttaccgagc cccattgatt tcaggaagct ggttgggtag agcatttcct gcatccccgg
    49681 accacctttt gggccttcat agcgaattac taccacatct ccggcgacaa ctttaccgcc
    49741 gagaatcgct tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc cggtgaattt
    49801 gaggatgctg tcatcgacgc ccgcagtttt aacgatgcag ccgttttccg cgaaattacc
    49861 gtagagcacc gccaggccgc cgtctttgct ataggcgtgt tccagcgagc ggatacagcc
    49921 attggcgcga tcgtcgtcca gcgtatccca acggcaatct tgcgagaatg cctgggtggt
    49981 acggataccc gcagggccgg cgcggaacat attttttacc gcgtcatcct gggtcagcat
    50041 aacgtcgtat tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg
    50101 gttcagtaac cccgcacgat ccagctcgcc gagaataccg ataacaccac cagcacggtg
    50161 aacatcttcc atatggtatt tttgggtgct cggcgcgact ttgcataact gcggaacctt
    50221 gcgggaaagc ttatcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc
    50281 cagcaggtga agtacggtgt tagtcgatcc acccatcgcg atatccagcg tcatggcatt
    50341 ttcaaacgcc gctttgctag caatattgcg gggcagtgca ctttcgtcgt tttgctcgta
    50401 gtaacgtttg gtcaattcaa caatgcgttt accagcattg aggaacagct gcttacggtc
    50461 agagtgggtt gccagcagcg agccgttgcc cggctgcgac agacccagcg cttcggtcag
    50521 gcagttcatt gagttagcgg taaacatccc ggagcaggaa ccgcaggttg ggcatgcgga
    50581 acgttcaacc tgatcgctct gggagtcaga tactttcggg tctgcgccct ggatcatcgc
    50641 atcaaccaga tcgagcttga tgatctgatc ggacagtttg gttttcccgg cctccatcgg
    50701 gccgccggaa acaaagatca ccggaatatt cagacgcagg gaagccatca gcatccccgg
    50761 ggtgattttg tcacagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat
    50821 atactcaacg gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg
    50881 gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc
    50941 cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac
    51001 aaattgggtg aacgagttca caaccgcgat aatcggctta ccgaaatcgg cgtcggtcat
    51061 tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc
    51121 ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg
    51181 cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc catttatctt
    51241 cggtttcgcc ggtaaagagg ccgaagaagg cttgctgaat gcgtttggtg accgggccac
    51301 aacggccttc gccaacctga ataccgtcta cactgcgcac tggcgtgatt tctgccgccg
    51361 taccggacat aaagacttca tccgccaggt acaaggattc gcgcgacagc acctgctcac
    51421 gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca
    51481 gcgcggagga ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt
    51541 cgcctgcgcc ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt
    51601 ggcggcgcgc ttcgctaccc accagcaggg aagagagata gttaccaccg gcttttgccg
    51661 cagttgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct
    51721 gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcggcgata atcacatcgg
    51781 ttgagtatcc cgctggcggg tttacgccca tgccaacatc accaacgaag atcagcggac
    51841 ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acggcaagct tccatcagct
    51901 catcaatgct ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca
    51961 tatgctcacg atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt
    52021 caaaaaccga ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc
    52081 agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat
    52141 tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg
    52201 tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg
    52261 gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag
    52321 cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttccggattg
    52381 aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat
    52441 tactggcacc aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa
    52501 gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac
    52561 gggtgatgtg ttggccaggg atgccgaagg cgctggctaa cttgaggaaa tcggggttat
    52621 cggtaagggt ggtttcgctg tatcgttcct ggaaaaacag ttgctgccat tgtcgaacca
    52681 tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg
    52741 tgcccagctc ttgtacattc atcatgaaag agccgtcacc ggagatacag acgacggtat
    52801 cgtttggtcg cgcgacttgc gcgccaaccg ccgccggtaa accaaaaccc atggtgccta
    52861 agccgctgga ggtaatgaaa ttttccgggc gagtgtgtgc gatgtgctgc gcggcccaca
    52921 tctgatgctg ccccacatct gtggtcacaa cgcaatccgc aggtttacga tccgacagtt
    52981 gttttaacaa caacggcgcg tagatagcgt caccgggatg gtcgtaacgc caggcatgtt
    53041 catcacgcag ctgcgcgcag tattgctgcc agtcattgat atttaatggc tgctgtaatg
    53101 ctggtaacag cgcatttaaa tcaccctgta atgccacatg tgcctgacgc agcttgttca
    53161 tttctgccgg gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcaatt
    53221 tgccggtcac ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg cactcctgca
    53281 ccgcgaagtt cgccgctttg gtgccatgca ttcccagcat gcccagatag tacggataat
    53341 ctgcttcaac tgcgcccagc cctttcagcg tgcaggtggc aggcattttt gtggtagcga
    53401 gaaattctcg taaagcagga actgcctgcg ccatacccac gccaccacca acgtacagca
    53461 tcggtttttg cgcttttgcc agcatctggc gcgcttgctc aacttcggca tgtgggaaag
    53521 tcacttcgtt ttcaacggtg gtgaaccacg gttccaggtc gccgctggct aactggatat
    53581 cttttgggat atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt
    53641 cagccataat gcgcggcaac tcttccagcg actgcaccag aaagctgtgc ttggtacagg
    53701 ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc agtaccgata aacggtgcgg
    53761 acacttgacc ggtgatggca acaacaggga tagaatctaa cagtgcgtcc gcaagcccgg
    53821 ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag
    53881 cacgggcata accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct
    53941 ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga
    54001 aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag
    54061 ttagttcccc gtcctgaatc ttgggaaaca gaattttgtg ctgttattca ttgtctgctc
    54121 ctcggttatg tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg gggtcttagt
    54181 tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata
    54241 ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg
    54301 tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accatagatt ttgcgaatag
    54361 cacaagatat tttttggccg ttttttatga tggctttaat aatttcaata gaaattgttg
    54421 ttttttaaaa agaaaatagt gagtgaaaaa aattcaattt cagtacgaat gttgttccct
    54481 ccctcgtagt tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca
    54541 actcaacttc acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg
    54601 ctacagcagc gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca
    54661 attgttcata cccgcgcagc cctgggagta aatgcgcccc caatcactgt tgaggtacat
    54721 atcagtaaag gtttgcccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa
    54781 gctcgcgatc gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa
    54841 atcaccatta accttgctcc agccgatcta ccaaaagaag ggggacgata tgatttacct
    54901 atcgccattg cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat
    54961 gaattagtcg gagaactggc gcttacaggc gctctgcgtg gcgttcccgg tgcaatctcc
    55021 agtgcaactg aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat
    55081 gaagtggggc taattaacgg tgaaggatgc ctggtagccg atcatctgca agctgtctgt
    55141 gcgtttctgg aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg
    55201 gcgctacaac atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg aggactggaa
    55261 attaccgccg ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca
    55321 atgctcgcca gccgtattaa tggtcttttg ccagatttaa gcaatgaaga ggcactggag
    55381 agcgctgcga tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc
    55441 ccgttccgct cacctcatca cagtgcgtcg ttaacggcga tggtgggcgg tggtgcaatt
    55501 ccagggccag gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct
    55561 gaatttgaac ggcgtacact ggatgctttg cgagagccga ttgaatccgg gcagatccat
    55621 ctttcacgca cacgagcaaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg
    55681 aatcccagcc ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca
    55741 ttacgttatc tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag
    55801 atcccattac caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcacc
    55861 accgttaaac aacgtgtaat ggctgccaga gagcgccaat ttaagcggca gaataagttg
    55921 aacgcctggc tggatagtcc ggaaatacgc aaattctgca agcttgagag cgaagatgcg
    55981 cagtggctgg aagaaacgct gatccatctg gggttatcga ttcgtgcctg acagcggtta
    56041 ttgaaagttg cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat
    56101 ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta
    56161 ctgacataaa aaaagggcat ttcgcccttt ttattaatcg tcagaatcgg tgtagtcttc
    56221 agcaccttca acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttgat
    56281 acgcgtcata tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg
    56341 gcatactgct acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa
    56401 ctcattgaag gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc
    56461 atggcgagag aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa
    56521 gctttccgcc atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat
    56581 tagttacgct tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc
    56641 ggagagcact gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca
    56701 ctttggtcga gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag
    56761 acaactggaa aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt
    56821 aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc
    56881 cgcccgtaag gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc
    56941 ctcattgtgg gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa atcaggatgc
    57001 ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct
    57061 gcatgaacgc cagcttgatc ttcttattac cactgaagcg cccaaaatgg acgaatttag
    57121 tagtcagttg ctggggtatt tcactttagc gctttatacc agtgcccctt ctaaactaaa
    57181 gggagatctt aactatctgc gactggagtg ggggccagat tttcaacagc atgaggcagg
    57241 tttgatcggt gctgacgaag tgcccattct gacaaccagt tctgctgaac tggcacagca
    57301 acagattgcg atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg
    57361 cggcctgcat accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct
    57421 gcaaaatagc gataaaaata cgttgattcg cgatctattg aaaattaacg tgctggatga
    57481 agtgtattaa tctgaatggc tggcaaggat gccggtagaa ggatttactt cagagagggt
    57541 tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga
    57601 gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc
    57661 taattag
//
LOCUS       DAZGXS010000013        55430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGXS010000013 DAZGXS010000000
VERSION     DAZGXS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(38..1048)
                     /gene="ascG"
                     /locus_tag="ACHKG5_000949"
     CDS             complement(38..1048)
                     /gene="ascG"
                     /locus_tag="ACHKG5_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0867100.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            1308..2765
                     /gene="ascF"
                     /locus_tag="ACHKG5_000950"
     CDS             1308..2765
                     /gene="ascF"
                     /locus_tag="ACHKG5_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0867101.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            2774..4198
                     /gene="ascB"
                     /locus_tag="ACHKG5_000951"
     CDS             2774..4198
                     /gene="ascB"
                     /locus_tag="ACHKG5_000951"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0867102.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(4322..4792)
                     /gene="hycI"
                     /locus_tag="ACHKG5_000952"
     CDS             complement(4322..4792)
                     /gene="hycI"
                     /locus_tag="ACHKG5_000952"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0867103.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(4785..5195)
                     /gene="hycH"
                     /locus_tag="ACHKG5_000953"
     CDS             complement(4785..5195)
                     /gene="hycH"
                     /locus_tag="ACHKG5_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0867104.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(5192..5959)
                     /gene="hycG"
                     /locus_tag="ACHKG5_000954"
     CDS             complement(5192..5959)
                     /gene="hycG"
                     /locus_tag="ACHKG5_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0867105.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(5959..6501)
                     /gene="hycF"
                     /locus_tag="ACHKG5_000955"
     CDS             complement(5959..6501)
                     /gene="hycF"
                     /locus_tag="ACHKG5_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0867106.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(6511..8220)
                     /gene="hycE"
                     /locus_tag="ACHKG5_000956"
     CDS             complement(6511..8220)
                     /gene="hycE"
                     /locus_tag="ACHKG5_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0867107.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(8238..9161)
                     /gene="hycD"
                     /locus_tag="ACHKG5_000957"
     CDS             complement(8238..9161)
                     /gene="hycD"
                     /locus_tag="ACHKG5_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0867108.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(9164..10990)
                     /gene="hycC"
                     /locus_tag="ACHKG5_000958"
     CDS             complement(9164..10990)
                     /gene="hycC"
                     /locus_tag="ACHKG5_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0867109.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YVLMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(10987..11598)
                     /gene="hycB"
                     /locus_tag="ACHKG5_000959"
     CDS             complement(10987..11598)
                     /gene="hycB"
                     /locus_tag="ACHKG5_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0867110.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(11723..12184)
                     /gene="hycA"
                     /locus_tag="ACHKG5_000960"
     CDS             complement(11723..12184)
                     /gene="hycA"
                     /locus_tag="ACHKG5_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0867111.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            12396..12746
                     /gene="hypA"
                     /locus_tag="ACHKG5_000961"
     CDS             12396..12746
                     /gene="hypA"
                     /locus_tag="ACHKG5_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0867112.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            12750..13622
                     /gene="hypB"
                     /locus_tag="ACHKG5_000962"
     CDS             12750..13622
                     /gene="hypB"
                     /locus_tag="ACHKG5_000962"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0867113.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            13613..13885
                     /gene="hypC"
                     /locus_tag="ACHKG5_000963"
     CDS             13613..13885
                     /gene="hypC"
                     /locus_tag="ACHKG5_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0867114.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            13885..15006
                     /gene="hypD"
                     /locus_tag="ACHKG5_000964"
     CDS             13885..15006
                     /gene="hypD"
                     /locus_tag="ACHKG5_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0867115.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            15003..16013
                     /gene="hypE"
                     /locus_tag="ACHKG5_000965"
     CDS             15003..16013
                     /gene="hypE"
                     /locus_tag="ACHKG5_000965"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0867116.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            16087..18165
                     /gene="flhA"
                     /locus_tag="ACHKG5_000966"
     CDS             16087..18165
                     /gene="flhA"
                     /locus_tag="ACHKG5_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0867117.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(18202..18555)
                     /gene="ygbA"
                     /locus_tag="ACHKG5_000967"
     CDS             complement(18202..18555)
                     /gene="ygbA"
                     /locus_tag="ACHKG5_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0867118.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(18632..18766)
                     /locus_tag="ACHKG5_000968"
     CDS             complement(18632..18766)
                     /locus_tag="ACHKG5_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867119.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            18842..21403
                     /gene="mutS"
                     /locus_tag="ACHKG5_000969"
     CDS             18842..21403
                     /gene="mutS"
                     /locus_tag="ACHKG5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0867120.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            21509..22165
                     /gene="pphB"
                     /locus_tag="ACHKG5_000970"
     CDS             21509..22165
                     /gene="pphB"
                     /locus_tag="ACHKG5_000970"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0867121.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(22206..22442)
                     /locus_tag="ACHKG5_000971"
     CDS             complement(22206..22442)
                     /locus_tag="ACHKG5_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0867122.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(22453..23880)
                     /locus_tag="ACHKG5_000972"
     CDS             complement(22453..23880)
                     /locus_tag="ACHKG5_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0867123.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(23880..24473)
                     /locus_tag="ACHKG5_000973"
     CDS             complement(23880..24473)
                     /locus_tag="ACHKG5_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0867124.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            24620..25027
                     /locus_tag="ACHKG5_000974"
     CDS             24620..25027
                     /locus_tag="ACHKG5_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0867125.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(25147..26139)
                     /gene="rpoS"
                     /locus_tag="ACHKG5_000975"
     CDS             complement(25147..26139)
                     /gene="rpoS"
                     /locus_tag="ACHKG5_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0867126.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(26202..27296)
                     /gene="nlpD"
                     /locus_tag="ACHKG5_000976"
     CDS             complement(26202..27296)
                     /gene="nlpD"
                     /locus_tag="ACHKG5_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0867127.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(27481..28107)
                     /gene="pcm"
                     /locus_tag="ACHKG5_000977"
     CDS             complement(27481..28107)
                     /gene="pcm"
                     /locus_tag="ACHKG5_000977"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0867128.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(28101..28862)
                     /gene="surE"
                     /locus_tag="ACHKG5_000978"
     CDS             complement(28101..28862)
                     /gene="surE"
                     /locus_tag="ACHKG5_000978"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0867129.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(28843..29892)
                     /gene="truD"
                     /locus_tag="ACHKG5_000979"
     CDS             complement(28843..29892)
                     /gene="truD"
                     /locus_tag="ACHKG5_000979"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0867130.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(29889..30368)
                     /gene="ispF"
                     /locus_tag="ACHKG5_000980"
     CDS             complement(29889..30368)
                     /gene="ispF"
                     /locus_tag="ACHKG5_000980"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0867131.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(30368..31078)
                     /gene="ispD"
                     /locus_tag="ACHKG5_000981"
     CDS             complement(30368..31078)
                     /gene="ispD"
                     /locus_tag="ACHKG5_000981"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0867132.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(31097..31408)
                     /gene="ftsB"
                     /locus_tag="ACHKG5_000982"
     CDS             complement(31097..31408)
                     /gene="ftsB"
                     /locus_tag="ACHKG5_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0867133.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(31602..31925)
                     /gene="ygbE"
                     /locus_tag="ACHKG5_000983"
     CDS             complement(31602..31925)
                     /gene="ygbE"
                     /locus_tag="ACHKG5_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0867134.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(31975..32580)
                     /gene="cysC"
                     /locus_tag="ACHKG5_000984"
     CDS             complement(31975..32580)
                     /gene="cysC"
                     /locus_tag="ACHKG5_000984"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0867135.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(32580..34007)
                     /gene="cysN"
                     /locus_tag="ACHKG5_000985"
     CDS             complement(32580..34007)
                     /gene="cysN"
                     /locus_tag="ACHKG5_000985"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0867136.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(34009..34917)
                     /gene="cysD"
                     /locus_tag="ACHKG5_000986"
     CDS             complement(34009..34917)
                     /gene="cysD"
                     /locus_tag="ACHKG5_000986"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0867137.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            35169..36206
                     /gene="iap"
                     /locus_tag="ACHKG5_000987"
     CDS             35169..36206
                     /gene="iap"
                     /locus_tag="ACHKG5_000987"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0867138.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGIIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   36288..36500
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=36349..36378
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(36596..36889)
                     /gene="cas2e"
                     /locus_tag="ACHKG5_000988"
     CDS             complement(36596..36889)
                     /gene="cas2e"
                     /locus_tag="ACHKG5_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0867139.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(36886..37809)
                     /gene="cas1e"
                     /locus_tag="ACHKG5_000989"
     CDS             complement(36886..37809)
                     /gene="cas1e"
                     /locus_tag="ACHKG5_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0867140.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(37806..38456)
                     /gene="cas6e"
                     /locus_tag="ACHKG5_000990"
     CDS             complement(37806..38456)
                     /gene="cas6e"
                     /locus_tag="ACHKG5_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0867141.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(38438..39184)
                     /gene="cas5e"
                     /locus_tag="ACHKG5_000991"
     CDS             complement(38438..39184)
                     /gene="cas5e"
                     /locus_tag="ACHKG5_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0867142.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMLKEVRKARYFSRREELSAPA
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(39195..40250)
                     /gene="cas7e"
                     /locus_tag="ACHKG5_000992"
     CDS             complement(39195..40250)
                     /gene="cas7e"
                     /locus_tag="ACHKG5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0867143.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(40262..40798)
                     /gene="casB"
                     /locus_tag="ACHKG5_000993"
                     /gene_synonym="cse2"
     CDS             complement(40262..40798)
                     /gene="casB"
                     /locus_tag="ACHKG5_000993"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0867144.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(40795..42357)
                     /gene="casA"
                     /locus_tag="ACHKG5_000994"
                     /gene_synonym="cse1"
     CDS             complement(40795..42357)
                     /gene="casA"
                     /locus_tag="ACHKG5_000994"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0867145.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(42608..43342)
                     /gene="cysH"
                     /locus_tag="ACHKG5_000995"
     CDS             complement(42608..43342)
                     /gene="cysH"
                     /locus_tag="ACHKG5_000995"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0867146.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(43416..45128)
                     /gene="cysI"
                     /locus_tag="ACHKG5_000996"
     CDS             complement(43416..45128)
                     /gene="cysI"
                     /locus_tag="ACHKG5_000996"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0867147.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(45128..46927)
                     /gene="cysJ"
                     /locus_tag="ACHKG5_000997"
     CDS             complement(45128..46927)
                     /gene="cysJ"
                     /locus_tag="ACHKG5_000997"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0867148.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            47246..47608
                     /gene="queD"
                     /locus_tag="ACHKG5_000998"
     CDS             47246..47608
                     /gene="queD"
                     /locus_tag="ACHKG5_000998"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0867149.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            47686..48956
                     /gene="ygcN"
                     /locus_tag="ACHKG5_000999"
                     /pseudo
     CDS             47686..48956
                     /gene="ygcN"
                     /locus_tag="ACHKG5_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            48947..49207
                     /gene="ygcO"
                     /locus_tag="ACHKG5_001000"
     CDS             48947..49207
                     /gene="ygcO"
                     /locus_tag="ACHKG5_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0867150.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            49224..49799
                     /gene="ygcP"
                     /locus_tag="ACHKG5_001001"
     CDS             49224..49799
                     /gene="ygcP"
                     /locus_tag="ACHKG5_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0867151.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(49947..50807)
                     /gene="ygcQ"
                     /locus_tag="ACHKG5_001002"
                     /pseudo
     CDS             complement(49947..50807)
                     /gene="ygcQ"
                     /locus_tag="ACHKG5_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(50804..51583)
                     /gene="ygcR"
                     /locus_tag="ACHKG5_001003"
     CDS             complement(50804..51583)
                     /gene="ygcR"
                     /locus_tag="ACHKG5_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0867152.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(51561..52897)
                     /gene="ygcS"
                     /locus_tag="ACHKG5_001004"
                     /pseudo
     CDS             complement(51561..52897)
                     /gene="ygcS"
                     /locus_tag="ACHKG5_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(52991..54445)
                     /gene="ygcU"
                     /locus_tag="ACHKG5_001005"
     CDS             complement(52991..54445)
                     /gene="ygcU"
                     /locus_tag="ACHKG5_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0867153.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(54515..55300)
                     /gene="ygcW"
                     /locus_tag="ACHKG5_001006"
     CDS             complement(54515..55300)
                     /gene="ygcW"
                     /locus_tag="ACHKG5_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0867154.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT        13002 a        15215 c        14277 g        12936 t
ORIGIN      
        1 catcgacact agtgacgatg acatgtgatg acaatgttta tcgcgaaaga gcaatgagtg
       61 agtcgcggcg gatcagtttt ccgctgaagg ttttcggcgg tgagaaatcc ccgccatcga
      121 gcataaaaat cagccgtcca ataatttcct gaatcatctc agtcaccgga atttttacgc
      181 tggagagcgc cggaacgata tagggggcaa ttgcgatatc atcgaatccg ataactgaca
      241 cctgctctgg caccgctacg ccgcgcttgt gtaacgcttt catcgcacct atcgccatat
      301 cgtcgttact ggcaactaac gcgctaaatt tagccccacg ttcgagcaac gtttctaccc
      361 cttcggcccc gctggcgggc gtccatttac cgttagcgat aagtttttca ttgagcgcaa
      421 taccatgctg cgccagcgcg tctttatatc cggcaagacg ttcaacgctg gtgggggaat
      481 ccatcgagcc ggtaagaaaa gcaatctcct gatgcccggc gtttatcaac tctgccacgg
      541 cgttaaagct ggtctgttta tgatcgcacc agacgctatg gctgctattt ttgcgcaggc
      601 ggcgattaag caccattatc ggctgactgt gcgcgtcaat gatgtcatcg atctcatcca
      661 cacttaaaaa acgcgggtaa atcataatgg cgtcgcagcg caaatccagc agatactgaa
      721 tcgcctggcg ctcttcttcg gcgctgtgtt taccatctgc caatagcaac tgccgccctt
      781 tctcttccgc cattcgcgcg gcatgaaaca gtaattcact aaaataaatg ccgtggtaaa
      841 gcgtgttagt cactaccagc cccagcgtct gggtactttt cgccgacaga ttgcgcgcca
      901 gcaagtttgg acggtaaccg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct
      961 ggctgacgta gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc
     1021 gcttcgccac ttccagcatc gtcgtcatca ttttcatccc tttacacgca atcaacgcag
     1081 tgtactgcac cgtttaccga ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc
     1141 ggtttcacaa atataaaaaa tgaacaatta actctcttgc ttatttggtt acaactattc
     1201 atgattttgt gaaaccggtt tcttaattcc gtttcagcat cggcattttt ccgtcacgtc
     1261 gactgataac aactacatct tccctactga taacaggata aaatccgatg gccaaaaatt
     1321 atgcggcgct ggcacgctcg gtgatagcgg cactgggcgg cgttgataac atctcggcgg
     1381 tcacgcactg tatgacgcgt ttgcgctttg ttatcaaaga tgatgcgctt atcgacagcc
     1441 cgacgttaaa aaccatcccc ggcgtgctcg gcgtggtacg tagtgacaac cagtgtcagg
     1501 tgattattgg caataccgtt tcacaagcct ttcaggaagt cgtcagcctg ctgccgggag
     1561 atatgcagcc cgccctgccc gtgggtaaac ccaaactcac gctgcgtcgc attggtgcgg
     1621 ggatcctcga tgcgctgatc ggcaccatgt caccgctgat cccggcgatt atcggcggat
     1681 cgatggtcaa actgctggcg atgatcctcg agatgagcgg cgtgctgaca aaaggatcgc
     1741 cgaccttaac cattctgaat gtgattggtg acggtgcttt cttcttcctg ccgctgatgg
     1801 tcgcggcttc ggccgccatc aaatttaaaa ccaatatgtc gctggcgatt gccatcgcgg
     1861 gtgtgctggt acatccgagt tttattgaac tgatggcgaa agcggcccag ggtgaacatg
     1921 tcgaatttgc cctgattcca gtcaccgcgg tgaaatacac ctacacagtg atcccggcgc
     1981 tggtgatgac ctggtgcctg tcatatatcg aacgctgggt ggatcgcatt acaccggcgg
     2041 tgaccaaaaa cttcctcaag ccaatgctga ttgtgttgat tgccgcaccg ctggcaatcc
     2101 tgctgattgg cccgattggt atctggatcg gtagcgccat ttcggcgctg gtttacacca
     2161 ttcatggtta tctgggctgg ctttcagtcg ccattatggg cgcgctgtgg cctctgctgg
     2221 taatgaccgg gatgcaccgc gtctttacgc taaccatcat tcagaccatt gccgaaaccg
     2281 gcaaagaagg gatggtcatg ccgtcagaga tcggcgctaa cctgtcgctg ggcggttcat
     2341 cactggcggt ggcgtggaaa acgaaaaacc cggaactgcg ccagactgcg ctggctgcgg
     2401 cggcatcagc cattatggcg gggatttccg aaccggcgtt atacggcgtg gcgatccgcc
     2461 tgaaacgtcc gcttatcgcc agtcttatca gcggttttat ttgcggcgcg gttgccggta
     2521 tggcggggct tgccagccac tcaatggcag cgccggggct atttaccagc gtgcagttct
     2581 tcgatccggc gaatccaatg agcatcgtct gggtgttcgc ggtcatggcg ctggcggtgg
     2641 tgctgtcgtt tatcctcaca ctgttgctcg gctttgagga tattcctgtt gaggaagcgg
     2701 ctgccgaggc gcgaaagcat cagagcgcac aaccgaccgt cgccaaagaa gtaagtctta
     2761 attgaggatg aaaatgtcag tatttccaga aggtttttta tggggcggcg cgcttgccgc
     2821 caaccagtct gaaggtgcgt tccgtgaagg tggcaaagga ctgacaacgg tcgatatgat
     2881 cccacacggc gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt ttcagttgcg
     2941 cgatgacgag ttttatccca gccatgaggc gacgggtttt tatcatcgtt ataaagaaga
     3001 tatcgccctg atggcagaga tgggattcaa agtgttccgt acctcaattg cctggagccg
     3061 ccccttcccg cagggtgatg aactgacgcc caaccagcag ggcattgctt tttaccgttc
     3121 tgtctttgaa gagtgtaaaa agtacggtat cgaaccgctg gtcacattgt gccacttcga
     3181 tgtgccgatg cagctggtca ccgaatatgg ctcctggcgt aaccgcaagc tggtggagtt
     3241 tttcagccgc tacgccagga cctgctttga agcatttgat ggtctggtga aatactggct
     3301 aaccttcaat gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg ggctggtgtt
     3361 tgaagaaggt gaaaatcagg atcaggtgaa atatcaggcc gcgcatcacc agctggttgc
     3421 cagtgcgcta gccaccaaaa tcgcccatga ggttaacccg caaaatcagg tgggctgtat
     3481 gctggcgggc ggtaacttct acccttacag ttgcaagccg gaagatgtct gggcggcgct
     3541 ggagaaagat cgggaaaacc tgttttttat cgatgtgcag gcgcggggcg cgtatccggc
     3601 ttactctgct cgcgtattcc gcgaaaaagg ggtaaccatc gacaaagcac cgggcgatga
     3661 tgagatcctg aaaaacaccg tcgattttgt ctctttcagc tattacgcct cgcgctgcgc
     3721 ctcggcggag atgaacacca acaatagcag tgcggcgaac gtagtgaaat cgctgcgtaa
     3781 cccgtatcta caggtgagcg actggggctg gggaattgat ccactcggtc tgcgtatcac
     3841 catgaacatg atgtacgacc gctatcagaa gccgctgttt ctggtggaaa acggcctcgg
     3901 cgcaaaagat gaacttgctg ccaatggcga gattaacgat gactatcgca tcagctattt
     3961 acgcgaacat atccgcgcaa tgggcgaagc gattgcagat ggcattccgc tgatgggcta
     4021 caccacatgg ggctgtattg atttagtttc cgcctctacg ggtgaaatga gcaaacgcta
     4081 cggttttgtc tttgttgacc gtgacgacgc aggcaacggc acgctgacgc gcacacgtaa
     4141 gaaatcgttc tggtggtata aaaaagtgat tgccagtaat ggggaagatt tagagtaggt
     4201 aacctactgt cgcactaacc aggaaggccc gataagcgtt gcgcatcggg caatctgggg
     4261 tttgctatca gttctgattc cctcatcaag aacatccctg tcctgattac ttattgaaaa
     4321 actactcttc ttccaccgct aactgcgcga agccgccgtt cccttcccag ccttccagtc
     4381 gttgataaac ggtttctacc gcatctttaa tcggctgggt catcgggtag taaaagccga
     4441 cgatatccgg ctggatgccg aggaaaatca cttcgccaat atcttctttc aactgatcga
     4501 taaggtagtt caacggcatg ttatgggtag tcatcataaa catctcggcg atatcatccg
     4561 ggtcgatgat gcggatttcg ccggggttta gccccatatc cgtggcgtcg acaatcagca
     4621 gccgtgtcgg gcgcagttcg cggatagcga caatgtcgtt ttccggtgcg ctaccgccgt
     4681 caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc agcggacctg
     4741 cgccatcatc acccatcatg ctattgccaa cacagagtaa aacgtcagtc acgcagtctc
     4801 ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact taacagcatt
     4861 gtactccatt cctgctgctg cgaagtttgc gctgcccggg ctttatcgaa tgcattggcc
     4921 agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac accttccatt
     4981 ttgcggcggg cttcgctgcc cgcctccaga gtggcaatcc atgccagata ttcatcccac
     5041 gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc aatcgccagg
     5101 ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc aataaattta
     5161 cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct tcaacaacat
     5221 gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc agccagcgtg
     5281 caacctgttc ttcgccctgc cctaactgtg taaggtaatc atcggcaatc tgacgaccgt
     5341 aacgataacc cgccagtcga cgtgcttcgc gatccacttt cacacgcagc ggctgcacca
     5401 tatcgccatg caggatctcc gccggttgtt catcctgctc acccggccca cgggcgtgaa
     5461 ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg gctggcgttg
     5521 gcgggcagcc agggatataa acatccaccg ggacgatttt atccgtaccg ccccacacgc
     5581 agtagagatc gtggaagatc ccgccgctgt taccgcaggc accgtaggag atacaaattt
     5641 tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca cgggtgaccg
     5701 cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta atgccgaagc
     5761 gttctgcatc gaacagcggc gacaaagtgg cgaaaatttc gatttcgcag ccgttgcagc
     5821 cgccgcagtc cacgcggtaa acgtaggcag aacgtttgat ttttttcagt aacgacgcct
     5881 tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg gcgtcacggg
     5941 ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt caatacggtc
     6001 ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc tttcgcggtg
     6061 gttttccgcg cggctgtcgc cattgtgctt aagcagcgca atggcgtagt cgatctcttt
     6121 ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc gggactgttg
     6181 caggaagtct tctttcttcc acaccgccag ttcgtactct tgcgacagtt tgattgccgc
     6241 cgtcgggcag acttcttcgc agcgtccgca gaagatgcag cgcccgagat tgaactccca
     6301 cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg ggcaggcatt
     6361 gacgcaggcc gcgcagccga tgcactgctg cgggttctgc tctggcttac cacggaagtt
     6421 tttatcaacc gcaatcggtt ccagcggata agacgaggtc gccgtgccgg ttttgatgac
     6481 ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt tacgctcaat
     6541 gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac gcacatcgac
     6601 cacggtcatg cggtcggtac aggagtagca agggtccaga ctaccgataa tcagcggcgc
     6661 atcagaaacg gtgttgccgc gcagcatgta gcgcagggtc ggccagttcg cgtaggtcgc
     6721 ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc agtggatatc
     6781 atcgccgcgc ggcgcttcgg caaagcccag cgcaaaacgg tgcggaatgt aggtaaagcc
     6841 ttccaccatc agcgggccgc ccggcaggtt atccagaccg tagtcgatca tgttcagcgc
     6901 ggtatagact tcgttaatac gcactttcag acgcgaaata acgtcgcagc cctgctcgct
     6961 gtggacttcc attggcagca ggccataacc aacaaacggg tgatcggcgc gggtatcgcg
     7021 ggcgtgaccg ctggcacgga ccatcgggcc gacgttactg aagtcgcgag cgatttccgg
     7081 gtccagacga ccaatgccga cagtgcgctg ttccatgttc ggcgtactca gcagcacatc
     7141 caccagctcc tgcacttcac gacgcatctg ttgcgccagt tgacgggtct ggatcatgtc
     7201 atctttcagc agatcgcgac gaatcccgcc gatcaggttc aggccgtagg ttttacgcgc
     7261 accggtaagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga actgcataaa
     7321 gccggagtca aagccggtaa agtgacaggc gaggccgagg ttgagcagat gcgagtgcag
     7381 acgttctacc tccagcagaa tggcgcggat catctgcgca cgttctggca cctgaatacc
     7441 catcgcgttt tccaccgacg tggtgtaggc ggtgctgtga gcaaaaccgc agatcccgca
     7501 cacacggtca gagaggaagg tcacttcgtt ataacccatg cgggtttctg ccagtttttc
     7561 catgccgcga tggacataga acaggcggta gtcggcgtcg ataatgtttt cgccatcgac
     7621 gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa tcggcacgac
     7681 gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat cggtggtcgg
     7741 tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt catccggcca
     7801 gtcatccggc agcaccagac gacgttcatc cggcaaacca accggaatca aaccgtacat
     7861 atcacgtact tcacgctcgc cccacaccgc agccggaacg cgcggcgtca cggaaggata
     7921 ctccggtttg ttggcatcaa cttcgacgcg aaccgttacc cagcacttag tccccttctc
     7981 catcgacagc acgtagtaaa cggcgtaatg accattcagt ttgcgttcgt cgttaccaaa
     8041 aagcaccgac agccagcccc cctgcttgta gtagagaaac tccaccactt ccggcagata
     8101 gttcaccttt acggtgacag tcagctgatc tttggtctgc caggcgtggt ccagcacgac
     8161 gcccggaaat gcctcattca gcgcggcgag ataatgttga cctaattttt cttcagacat
     8221 gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa tgcaaagcca
     8281 aacccagccc aggtaatgcg cggagtaata tcaagacgca gacgggccat gctgttttcg
     8341 aacagcgcga taaccaggac gccgaccacc agtttgacga tggcaatcac caacgccagc
     8401 agcagcccac cgacggtgaa ggtttccatt tgtccccacg gaataaacac cccgacgaac
     8461 atctgcagca ccaccaactg tttcaggctg ataccccatt tcatgacgcc aaagccgctg
     8521 ccgctgtact cagagagcgg accttcctgc aactcctgct cggcttccgc caggtcgaac
     8581 ggcagtttgc ccatttcgat aaaagtggcg aacgcacagg cacaaagcgc cagcaccagc
     8641 gggatgctct ggctcagcgg ccagtgataa acggtgtcgg tgatgttgct gatgttggtg
     8701 gaactggcaa cctgtgcggc aacccacaga ccaagcagca gcatcggttc gaccagcacg
     8761 ccaagcatcg cttcacggct cgcgccgata gcggtaaacg gactaccggt atccagacca
     8821 gaaatggcaa agaagaaacg cgcgatggca aagagataca gtaaggtgat caaatcaccc
     8881 agttgcggca gcggagaacc gacggtcacc accggcagcg cagtagcgat agtcagcatg
     8941 acgcccacca tcacatacgg cgtcaggcgg aacacccagc cggaggcatc cgggccaacg
     9001 ctctggcgac ccagcagttt gataatgtcg cgatactcct gcaacacgcc cggcccgcga
     9061 cggttatgca agcgggcgcg cgccacgcgg gtaataccgg agagcagcgg cgcaacggca
     9121 aatagcacca gcgcctgaat taacggatat aaaacactca ttctcaggct cctcgtgaaa
     9181 caataatcac caccagcacc gccagttcaa ccagcgccat ccggcggaac agcaacgcac
     9241 tgccttcgca ctgccagccc ggcaccagcg acaccggatt cagccatttg cgcagtttca
     9301 gcaccggcgc aaacgcctgt ttcaccggca tggcaaaacc gtgggcggta attaccattg
     9361 atttttcatg gtcgtagccg cacacccagg ccgcgccgcg ggaacgcgat ggcaaacgat
     9421 cgcctttgca aatcgccata atgatgaatg gcagcagcgg gcaggcaatc agcagcaacg
     9481 tgatcatcgg ttgagaaacg gtggtgttag caggttccag cggcagaggt acagcagcag
     9541 agagcatcgg cagcagccac ggcgcagcaa caccgccaat tacgcagcaa atcgccagtg
     9601 ccactacgct tacgctcatc aggagcggtg cacaggtggc gttttcggct tctttggtac
     9661 gcggcgcgcc gaggaacgtg acgccataga ctttcgccat acacatcacc gccagcgcac
     9721 cggtaattgc cagacccaca gcgagcagcg gccccagcag acgggcaaca aacgcgccac
     9781 tattgctcag tttgaagaag gattgataga taacccattc cccggcaaaa ccgttcagcg
     9841 gcggcagtgc agccattgcc atcagcccga ctaacatagc gatggagata accggcattt
     9901 tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac cagacgctcc
     9961 ccgccccgag gaacagtacg cttttgaaca ggctatggtt aagcagatgg tacagaccac
    10021 cgaccaggcc aagagcaatc agcgccggtt gttcgagcgc gatacccgtt acgccagcgc
    10081 ccagccccag caggataatg ccgatatttt ccagggtgtg gtaagccagt agacgctgga
    10141 tattatgttc catcaacaca tacagaccgc cgacaaacgc ggtgatcata ccgagcacca
    10201 gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg gttaaaatgc
    10261 ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc gcagaggcgt
    10321 tcgcatgtgc ctgcggcacc cagccgtgca gcggaataat cccggccagc aggccaaagc
    10381 caatcacact gagcagccag atatcggaac cgagcggcag ctgttgcata cgcatatcca
    10441 gcaggcgcag atccagcgtg ccgtaacgct gccacagcag ccagcaagca atcgccagca
    10501 gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg ttgctggtga
    10561 ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg aggttgctgg
    10621 caatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg ttgcacttca
    10681 cctgcgcgtg gcgatgccag tcaatgttgt agaggctgac aaacagaccg cacagaccga
    10741 gcgtaatcag ccaaatcgcg ttaagcggag agatttgcac atcgtagctt accagcgaca
    10801 gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct gccgtataca
    10861 gactaccaac cgcgccgcca attccggcta tccagccgct tagcgccttt tgaaaagaaa
    10921 agagaaatgc cagaacagcg gcggcgacaa accacgccac gccgctattg atcagggaaa
    10981 ttgcgctcat ttagcctctc cactttgagc ctgctgaaac aaggtgagat cgccaaagtc
    11041 cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg tgttatccac
    11101 cagatgcagg gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt catcaaagct
    11161 acaaaggtca catttgacgg cgatcgcgcg aatacccggc acccagtcaa gcagcgtact
    11221 gatacgcgcc ggagcaggcg gtgccggtgg cgctttcggg gtattggcat ttgcaggaat
    11281 atccagcgga cggctgccgg aaaattcaat tgcgccaaac gggcaggcga tgccgcacag
    11341 cttgcagctt acgcacaggc tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc
    11401 gttaaccggg cagaccaccg cgcagggtgc atcttcacag tggtgacaga gctgcggcgc
    11461 agattctttt tcatttagca tcactctcag gcgcggcatt gattgcaggc cgtgctggcg
    11521 atgcgtctct gaacaggcgg cctcacaagt gtggcaaccg atacagagcg tggagtcagc
    11581 aattacaaaa cgattcacca ggcattcctc aggtgattgt catttttgac gaaaacatgc
    11641 cgatgaaatg tcattttcga cactcatcga cacgcccatc cccaaacagg cgtaacgcct
    11701 gcaaaacggg caaagcctca gctcatgctg ccgggctttg tccctttacc agttggctta
    11761 aattcaccgg cacattgttt tcaatagcgg tgtataagct gtcgtaaacg ccagcgattt
    11821 tttcgagata gtgttccaga acctgttcgc gatcgcggat gttgaccttg ccatcaatca
    11881 tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt
    11941 cgacgtaata ctcgatggtg tggctgttaa agccatccgc cagggtgacg taaatacgaa
    12001 aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc gcagctcatg gcgtgatgca
    12061 ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt gcagtagatg tgccactggt
    12121 cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag
    12181 tcattgtcag gttacccgtt taacagagat gccagcttta agcatttttt gtgccaactt
    12241 ttaatttatt attattaaag cgatttttaa gattttggtg aatttcgtcg ccgtgtcgac
    12301 gtgtcatttc gacatcatcg acattattca ctgcagggat aatcaacact ggcacaatta
    12361 ttgcttgtag ctggcaatag ttaatgggag gcgatatgca cgaaataacc ctctgccaac
    12421 gggcactgga attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg
    12481 tctggctcaa aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg
    12541 atctggtttg ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa gaacaagagg
    12601 ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct actgacccag cgcgtccgcc
    12661 gctgtccaca gtgtcatggt gacatgctac agattgtggc agacgacggt ttacagattc
    12721 ggcggataga aatagaccag gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca
    12781 acctgtatat cgagggtgat gaacataacc ctcattccgc gtttcgtagc gcgccatttg
    12841 ccccggcggc acgcccgaag atgaaaatca ccggcattaa agcgcctgaa tttaccccca
    12901 gccagactga agaaggcgac ctgcattacg gtcatggcga agcgggcact cacgcaccgg
    12961 gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt gctggacaaa aataaccgtc
    13021 tggctgaacg caaccgcgcg cgctttgctg cctgcaagca actggtgctc aacctggttt
    13081 ccagcccagg ttccggtaaa accaccctgc tgacggaaac cttaatgcgc ctgaaagaca
    13141 gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc gcacgcattc
    13201 gcgctaccgg cacaccagcg attcaggtga acaccggtaa aggctgccat cttgacgcac
    13261 agatgattgc cgacgccgca ccgcgtctgc cactggacga taacggtatt ctgtttatcg
    13321 aaaacgttgg caacctcgta tgcccggcca gcttcgatct cggtgaaaaa cacaaagtgg
    13381 cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa atatccgcat atgtttgccg
    13441 ccgcctcgct gatgctgctc aacaaagttg acctgttgcc gtatctcaac tttgacgttg
    13501 agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat tgaaatcatc cttatttccg
    13561 ccaccagcgg cgaagggatg gaccagtggc tgaactggct ggagacacag cgatgtgcat
    13621 aggcgttccc ggccagatcc gcaccattga cggtaaccag gcgaaagtcg acgtctgcgg
    13681 cattcagcgc gatgtcgatt taacgttagt cggcagctgc gatgaaaacg gtcagccgcg
    13741 cgtgggccag tgggtactgg tacacgttgg ctttgccatg agcgtaatta atgaagccga
    13801 agcacgcgac actctcgacg ccttgcaaaa catgtttgac gttgagccgg atgtcggcgc
    13861 gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc gccggaacag
    13921 gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac atctctctta caccgccgaa
    13981 cgccctctgc ggattatgga agtgtgcggt ggtcataccc acgccatttt taaattcggc
    14041 ctcgaccagt tactgcctga aaacgttgag tttatccacg gtccgggttg cccggtgtgc
    14101 gtactgccga tgggcagaat cgacacctgc gtggagattg ccagccatcc ggaagtcatc
    14161 ttctgtacct ttggcgacgc catgcgcgtg ccggggaaac agggatcgct gttgcaggca
    14221 aaagcacgcg gtgccgatgt gcgcatcgtc tattcgccga tggatgcgtt gaaactggcg
    14281 caggagaatc caacccgcaa agtggtgttc ttcggcttag gttttgaaac caccatgccg
    14341 accaccgcca tcactctgca acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc
    14401 tgccagcata ttacgcttat cccgacgctg cgcagtttgc tggaacagcc ggataacggt
    14461 atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta tcggcactga tgcctataat
    14521 tttatcgcca gcgattttca gcgtccgctg gtggtggctg gtttcgaacc ccttgatcta
    14581 ctgcaaggcg tggtcatgct ggtggagcag aaaatagcgg cccacagcaa ggtagagaat
    14641 cagtatcgtc gggtggtacc ggatgccggt aacctgctgg cgcaacaggc gattgccgat
    14701 gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag gcgtgattga atcttctggc
    14761 gtgcacctga cgccggatta tcaacgattc gatgccgaag cacatttccg cccggcaccg
    14821 cagcaggtct gcgatgaccc gcgcgcgcgt tgtggcgaag tcttgacggg caaatgtaag
    14881 ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc aaaccgcgtt tggtgcgctg
    14941 atggtttcct ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa
    15001 gcgtgaataa tatccaactc gcccacggta gcggcggcca ggcgatgcag caattaatca
    15061 acagcctgtt tatggaagcc tttgccaacc cgtggctggc agagcaggaa gatcaggcac
    15121 gtcttgatct ggcgcagctg gtagcggaag gcgaccgtct ggcgttctcc accgacagtt
    15181 acgttattga cccgctgttc ttccctggcg gtaatatcgg caagctggcg atttgcggca
    15241 ccgcgaatga cgttgcggtc agtggcgcta ttccgcgcta tctctcctgt ggctttatcc
    15301 tcgaagaagg attgccgatg gagacactga aagccgtagt gaccagcatg gcagaaaccg
    15361 cccgcacggc aggcattgcc atcgttactg gcgatactaa agtggtgcag cgcggcgcgg
    15421 cagataaact gtttatcaac accgcgggca tgggcgcaat tccgacgaat attcactggg
    15481 gcgcacagac gctaaccgca ggcgatatat tgctggttag cggtacactc ggcgaccacg
    15541 gggcgactat ccttaacctg cgtgagcagc tggggctgga tggcgaactg gtcagcgact
    15601 gcgcggtgct gacgccgctt attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc
    15661 gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt
    15721 gcggtattga aatttctgaa tcagcgctgc cggttaaacc tgccgtgcgc ggcgtttgcg
    15781 aattgctggg actggacgcc ctgaactttg ccaacgaagg caaactggtg atcgccgttg
    15841 aacgcaacgc ggcagagcaa gtgctggcag cgttacattc ccatccactg gggaaagacg
    15901 cggcgctgat tggtgaagtg gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg
    15961 tgaaacgaac cctcgattta ccacacgccg aaccgcttcc gcgtatatgc taataaaatt
    16021 ctaaatctcc tatagttagt caatgacctt ttgcaccgct ttgcggtgct ttcctggaag
    16081 aacaaaatgt catatacacc gatgagtgat ctcggacagc aagggttgtt cgacatcact
    16141 cggacactat tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc gcaactggta
    16201 aagcgttctg cgctcgccga caacgcggct attgtgttgt ggcaagcgca gactcaacgt
    16261 gcgtcttatt acgcatcgcg tgaaaaagac acccccatta aatatgaaga cgaaactgtt
    16321 ctggcacacg gtccggtacg cagcattttg tcgcgccctg atacgttgca ttgcagttac
    16381 gaagaatttt gtgaaacctg gccgcagctg gccgcaggtg ggctataccc aaaatttggt
    16441 cactattgcc tgatgccact ggcggcggaa gggcatattt ttggtggctg tgaatttatt
    16501 cgttatgacg atcgcccctg gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag
    16561 atcgtttctg tcgtcaccga acaaatccag agtcgcgtcg ttaacaatgt cgactatgag
    16621 ttgttatgcc gggaacgcga taacttccgc atcctggtcg ccatcaccaa cgcggtgctt
    16681 tcccgcctgg atatggacga actggtcagc gaagtcgcca aagaaatcca ttactatttc
    16741 gatattgacg atatcagtat cgtcttacgc agccaccgta aaaacaaact caacatctac
    16801 tccactcact atcttgataa acagcatccc gcccacgaac agagcgaagt cgatgaagcc
    16861 ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc tgctgattaa tctccacgag
    16921 cgggatgatt tagcccccta tgaacgcatg ttgttcgata cctggggcaa ccagattcaa
    16981 accttgtgcc tgttaccgct gatgtctggc gacaccatgc tgggcgtgct gaaactggcg
    17041 caatgtgaag agaaagtgtt taccactacc aatctgaatt tactgcgcca gattgccgaa
    17101 cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg aaatccatcg tctgaaagaa
    17161 cggctggttg atgaaaacct cgccctgacc gagcagctca acaatgttga tagtgaattt
    17221 ggcgagatta ttggccgcag cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg
    17281 gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg gcacgggtaa agagctgatt
    17341 gcccgtgcta tccataatct cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc
    17401 gcggcgatgc ctgccggatt gctggagagc gatctgtttg gtcatgagcg tggggctttt
    17461 accggtgcca gcgcccagcg tatcggtcgt tttgaactgg cggataaaag ctccctgttc
    17521 ctcgacgaag tgggcgatat gccactggag ttacagccga agttgctgcg tgtattgcag
    17581 gaacaggagt ttgaacgcct cggcagcaac aaaatcattc agacggacgt gcgtctaatc
    17641 gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc gtgagttccg tagcgatctc
    17701 tattaccgcc tgaacgtatt cccgattcac ctgccgccac tacgcgagcg tccggaagat
    17761 attccgctgc tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc
    17821 gacagcattc ctgccgagac gttgcgcacc ttgagcaata tggagtggcc gggtaacgta
    17881 cgcgaactgg aaaacgtcat tgagcgcgcg gtattgctaa cacgcggcaa cgtgctgcag
    17941 ctgtcattgc cagatattgc tttaccggag cctgaaacgc cgcctgccgc aacggttgtc
    18001 gctcaggagg gcgaagatga atatcagttg attgttcgcg tgctgaaaga aactaacggc
    18061 gtggttgccg ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg
    18121 tcacggatga agcgactggg aattgataaa tcggcattga tttaactgca aattgccgga
    18181 cagatctgcc tgtccggcat actattcacg aggttttttc ggacgatatt tttccggcag
    18241 ttctggcacc ggacacttgt catcgatgag atgacgcacg gttaagatcg gatgacgcca
    18301 cagcattctc ggcccggccc aacgcataat ctgtttcatc tcttcacgct ttgcaggctg
    18361 gtaacagtgc accggacact gcttacaggc tggtttctct tcgccgaaca cacatttatc
    18421 cagccgcttt tgcgcgtaaa caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg
    18481 cgggcatttc gcttgataaa gatcgatcat ttttttaatc gtcagttttt cacgagagat
    18541 acgcttgccg gacatactgc ctccacctca ttaagatgca tttatattac aacttaatct
    18601 taaagggcac tatgactcta aagaagaagg gttagccaac cgatacaatt ttgcgtactt
    18661 gcttcataag catcacgcaa aagctgcaaa acagcatctt tcccggaacc agcatcaaga
    18721 actcgccgtt cgcttcttcc cctgaaatga ttaactccgg tatcatgtgc gccttatgtg
    18781 attacaacga aaataaaaac catcacaccc catttaatat cagggaaccg gacataaccc
    18841 catgagtgca atagaaaatt tcgacgccca tacgcccatg atgcagcagt atctcaagct
    18901 gaaagcccag catcccgaga tcctgctgtt ttaccggatg ggtgattttt atgaactgtt
    18961 ttatgacgac gcaaaacgcg cgtcgcaact gctggatatt tcactgacca aacgcggtgc
    19021 ttcggcggga gagccgatcc cgatggcggg gattccctac catgcggtgg aaaactacct
    19081 cgccaaactg gtgaatcagg gcgagtccgt tgccatctgc gaacaaattg gcgatccggc
    19141 gaccagcaaa ggtccggttg agcgcaaagt tgtgcgtatc gttacgccag gcaccatcag
    19201 cgatgaagcc ctgttgcagg agcgtcagga caacctgctg gcggctatct ggcaggacag
    19261 caaaggtttc ggctacgcga cgctggatat cagttccggt cgttttcgcc tgagcgaacc
    19321 ggctgaccgg gaaacgatgg cggcagaact gcaacgcact aatcctgcgg aactgctgta
    19381 tgcagaagat tttgctgaaa tgtcgttaat tgaaggccgt cgcggcctgc gccgtcgccc
    19441 gctgtgggag tttgaaatcg acaccgcgcg ccagcagttg aatctgcaat ttgggacccg
    19501 cgatctggtc ggttttggcg tcgagaacgc gccgcgcgga ctttgtgctg ccggttgtct
    19561 gttgcagtat gcgaaagata cccaacgtac gactctgccg catattcgtt ccatcaccat
    19621 ggaacgtgag caggacagca tcattatgga tgccgcgacg cgtcgtaatc tggaaatcac
    19681 ccagaacctg gcgggtggtg cggaaaatac gctggcttct gtgctcgact gcaccgtcac
    19741 gccgatgggc agccgtatgc tgaaacgctg gctgcatatg ccagtgcgcg atacccgcgt
    19801 gttgcttgag cgccagcaaa ctattggcgc attgcaggat ttcaccgccg agttgcagcc
    19861 ggtactacgt caggtcggcg acctggaacg tattctggcg cgtctggcgt tgcgtaccgc
    19921 tcgcccacgc gatctggccc gtatgcgtca cgctttccag caactgccgg agctgcgtgc
    19981 gcagttagaa actgttgata gtgcaccagt acaggcgcta cgtgagaaga tgggcgagtt
    20041 tgccgagctg cgcgatctgc tggagcgagc aatcatcgac acaccgccgg tgctggtacg
    20101 cgacggtggt gttatcgcat caggctataa cgaagagctg gatgagtggc gcgcgctggc
    20161 tgacggcgcg accgattatc tggagcgtct ggaagtccgc gagcgtgaac gtaccggcct
    20221 ggacacgcta aaagttggct ttaatgcggt gcacggctac tacattcaaa tcagccgtgg
    20281 gcaaagccat ctggcaccta tcaactatat gcgtcgccag acgctgaaaa acgccgagcg
    20341 ctacatcatt ccagagctaa aagagtacga agataaagtc ctcacttcaa aaggcaaagc
    20401 actggctctg gaaaaacagc tttatgaaga gctgttcgac ctgctgttgc cgcatctgga
    20461 agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac gtgctggtga acctggcgga
    20521 acgggcctat accctgaact acacctgccc gaccttcatt gataaaccgg gcattcgcat
    20581 taccgaaggc cgccatccgg tggttgaaca ggtgctgaac gagccattta tcgccaaccc
    20641 gctgaatctg tcaccgcagc gccggatgtt gattattacc ggtccgaaca tgggcggtaa
    20701 aagtacctat atgcgccaga ccgcactgat tgcgctgatg gcctatatcg gcagctacgt
    20761 accggcgcaa aaagtcgaga ttggcccgat tgaccgtatc tttacccgcg taggggcagc
    20821 ggatgatctg gcttccgggc gttcaacctt tatggtggag atgaccgaaa ccgctaatat
    20881 tctgcataac gccaccgagt acagtctggt gctgatggat gagattgggc gcggaacgtc
    20941 cacttacgat ggtctgtcgc tggcgtgggc gtgcgcggaa aatctggcga ataagattaa
    21001 ggcgttgacg ctgtttgcca cccactattt cgagctgacc cagttaccgg agaaaatgga
    21061 aggcgtcgcc aacgtgcatc tcgatgcact ggagcacggc gacaccattg cctttatgca
    21121 tagcgtgcag gatggcgcgg cgagcaaaag ctacggcctg gcggttgcag ctctggccgg
    21181 cgtgccaaaa gaggttatta agcgcgcacg gcaaaaactg cgtgagctgg aaagcatttc
    21241 gccgaacgcc gccgctacgc aagtggatgg tacgcaaatg tctttgctgt ccgtaccgga
    21301 agaaacctcg cctgcagtcg aggcactgga aaacctcgat ccggattcac tcaccccgcg
    21361 tcaggcgctg gaatggattt atcgcctgaa gagtctggtg taataataat tcccgatagt
    21421 cttttgctat cgggaatatt aacgataact gacgaatcaa ataaaaatac cctgtataat
    21481 aggaaagctt attttacagg gtaaaaccat gccatctaca cgctatcaaa aaatcaatac
    21541 ccatcactat cgccatatat gggtcgttgg tgatattcat ggtgaatatc agttattaca
    21601 atcccgctta catcaactct cttttttccc cgaaaccgac ttacttattt ctgtcggcga
    21661 taatattgat cgtgggccgg agagtcttaa cgtcctgcgc ctgctaaacc agccctggtt
    21721 tatctccgtt aaaggcaacc acgaagcaat ggcgctggat gcgttcgcga ccggcgatgg
    21781 caatatgtgg cttgccagcg gtggtgactg gtttttcgat ttaaatgatt cagagcaaaa
    21841 agaagctaca gatctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
    21901 cgacaacata aaatatgtca tcgcacatgc agattatccg gggagtgaat atctctttgg
    21961 taaagaaata gcggagagcg aattactctg gcctgttgat cgtgtgcaga aatcgcttaa
    22021 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
    22081 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagcgggcg
    22141 gctgtcattt tataaaataa agtaatgagg ccgggtaaag caaagccact acccggcact
    22201 ttttattagc gcttaccttc cgccagcagc ggcgggatgc tcggcaccat tggcgcgtca
    22261 tcaatatctt tctgcgtcat gcggaacgct tcgggataat gttcgcggct ggtacggcgc
    22321 agcggttcgg tatcgcgcca ggtataaagg caatgctggc actgatatac cgtccagaca
    22381 tctttcaccg gcgatttcgc catcacttca atctgttcat cggcacaacg tggacaaatc
    22441 atcttgttct ccttatttac gtgcggccag catagcggtc agtttttcag cccaggcttt
    22501 ggtttccggc aaatccacca ccggctggct gtagtgacca cggttgtccg gggcgacagg
    22561 cgtggtggcg tcgataatca gcttgtcggt gatccccgcc gggcttgagc cagggtcgag
    22621 ttccagcacg gacatattcg gcaactgcac caaatcccct gccggattta ctttcgagga
    22681 gagcgcccac atcacctgcg gcaggttgaa cgggtcaacg tcttcatcga ccataatcac
    22741 catctttacg tagcccagac cgtgcggcgt ggtcatcgca cgcaggccca ccgcgcgggc
    22801 aaagccgccg tagcgttttt tggtggagat aatcgccagc aggccgtggg tgtacatggc
    22861 gtttaccgcc tgcacttccg ggaactcggc tttcagttgt tgatacagcg gcacacaggt
    22921 ggctggcccc atcaggtagt cgatttcggt ccacggcatg ccgaggtaca gcgattcgaa
    22981 aatcggcctg gtgcggtaag agactttatc gatacgcacc acggtcatgt tacgcccgcc
    23041 ggagtagtgc ccggtaaact caccgaacgg cccttcgatt tcgcgtttgc ggctttcgat
    23101 aaccccttcg aggatcactt ctgaacccca cggcacatca aaaccagtca atggcgcggt
    23161 ggcgatcggg tacgggcttt cgcgcagcgc gcctgccatt tcatactcag actgatcgta
    23221 tttcagcggc gtggcgccca taagggtgat gatcggatcg ttacccaggg tgatggcaat
    23281 cggcagatct tcaccgcgct cttccgcttt atgcagatgc agggcgatat cgtgcatcgg
    23341 caccggttgc aggccgagct tacgcttgcc cttcacttcc atgcggtaga taccgacgtt
    23401 ctgcttgccg aagttatccg ggtcgagcgg atcgcgggaa accacgcacg ctttgtcgag
    23461 atagaaaccg ccatcaccgt cgtttaaacg aaacagcggc aggatatcga acagattaat
    23521 ctcgtcacca tcgacggtgt tctgcgccca ggctggattg gcgcggcgct ccggggcgat
    23581 cgggaagtta tcccagcggc ggataaactc atcaatctgt tttttaaccg gggtgtttgg
    23641 cggcaggccc aaggaaatcg cgtggttctg ccaggaaccg atggtattca tcgccacgcg
    23701 ggcatcggta aaaccgcgaa tattatcaaa ccacagcgcc ggtgcaccat cgccgatacg
    23761 cccggtggcg ttggcagcgg cagccagatc cggttccgcg ttcacctctt cgctgatttt
    23821 cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat caaatgccat
    23881 tattcattct cctgagaaaa attccgggcc tgcggcaatc cttgccagcg cctggcgtag
    23941 ggatgttcaa ggccaaattg atccagcacg cgggctacca cgtggtggac aatgtcatct
    24001 accgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac gcccatgcgt
    24061 gaaagtgcga gcatattttc gagatggatg gtgctaagcg gcatttcacg cggcaccagc
    24121 accagtttgc ggccttcttt aagcacgacg tccgccgcgc gccctaccag gccatcagcg
    24181 taaccagcgc ggataccggc gagcgttttc atactgcacg gaataacgat catgccgtct
    24241 gtacgaaagg aacctgagga gatggtcgcc gcctgatccg ccgggttatg gctgaagtca
    24301 gcgagggcag caacatcgcg ggcgctgtaa ggcgtttcca gttcaatggt ggttttcgcc
    24361 cacttcgaca tcaccagatg agtctcgaca ttcggcatct cccgcagcgc ttgcagtaat
    24421 gccacaccaa gaggcgcacc ggtagcccct gtcatcccga cgatcagttt cattgcttac
    24481 tctcttaatt gttcgtatac gaacattatt attaaaatac gcctctgttc tcattgcggc
    24541 aagcactgaa aagtaacaac atccccttcg tcactgaaaa accgctatga tagcggcaga
    24601 tagtttgccc ggagtttaca tgacgttacg aaataaagcg ttccatcagt tacgacagct
    24661 ttttcagcag cacacggctc gctggcagca cgagttacct gacctgacca aaccacagta
    24721 tgcggtgatg cgcgccattg ctgataagcc tggtatcgaa caggtggcac tgatagaagc
    24781 agcagtcagc accaaagcaa cgttggcaga aatgttggca agaatggaga atcgtggctt
    24841 agtcagacgt gaacatgatg ccgctgacaa gcggcgtcgc tttgtctggc tgactgccga
    24901 aggagaaaag atacttgctg cggcgatacc gattggtgac agcgtggatg aggaattttt
    24961 ggggcgtttg agtgcagaag agcaggagct gtttgtgcag ctggtgcgca agatgatgaa
    25021 cacataggat gcaaattgcc gggtaggacg ctgacgtgtc ttatccaggc gacaaaaaca
    25081 caagcttccc aaacaaaaga aaaaggccag cctcgcttga gactggcctt tctgacagat
    25141 gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg
    25201 caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga ggccaatttc
    25261 acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag
    25321 tacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct gcttcatatc
    25381 gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga tgtccagcaa
    25441 cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct cgttaagacg
    25501 aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct cttccgcact
    25561 tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc
    25621 ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg cccgttcaat
    25681 cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa
    25741 cttctctacc gcacggatca gccccaggtt gccctcttcg ataaggtcca gcaacgccag
    25801 accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac tctcgatcat
    25861 ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc
    25921 ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg cgtccaacac
    25981 acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat cactgggttc
    26041 ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc
    26101 ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc tacccgtgat
    26161 cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg cggcaaataa
    26221 cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg
    26281 gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg ttgttcccgg
    26341 accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg tttgatgata
    26401 atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc atctgcggtc
    26461 gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa
    26521 gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga gataggcgta
    26581 ctggttgatg tactgctgac agtcggctcg gttgtgcttg ctgttggtac cgttacaggc
    26641 gctgtgaccg tggtcgcagt tggcttgttg ttcggcaaca ttttgttagc actctgttca
    26701 cccgaagact cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt
    26761 gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc atttccgcca
    26821 gtgattggcg taccggaagc attacccacc tgtaaggtct gaccgacgtt cagcgcgtat
    26881 ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt aatccaggcg
    26941 atatagaaaa gcgtgtcgcc ttttttcacg gtataggtac tgccgctata actgcctttc
    27001 ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg taccggctgc
    27061 tgagctactg gctgcactgg ctggatttgc ggttgttgag tagcctgaat ttgtggctgc
    27121 tgcaccggct gaatttgcgg ttgctgcgct gtagacgtcg tccccatttt cggcggcggc
    27181 gtaatcaaca taccagaatt ggtatgtgca ggcgcattgc cattaacgga gctgaccggt
    27241 gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag tgacaaagcc
    27301 gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc aggaaaaatt
    27361 ggttaataac cagtgacata attaccgtgc aaggcaccat actgaacact ggaaaagatg
    27421 ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa tccaggtatt tcctcacgtt
    27481 ttaagccagc tcacccttca ctaaagggac aaagcgcacg gcctccacgg tatcgataat
    27541 aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc ctacgggtaa
    27601 gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt ccggcggtgc
    27661 cgccgtaaca atgatagcgt caaacggcgc acgtgcctgc caaccttgcc atccatcgcc
    27721 atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgcg cctgccactg
    27781 caagccttta atccgttcaa ccgagcaaac atgctggaca agatgcgcca ggattgccgt
    27841 ttgatatccc gaaccggtgc caatttccag cacccgcgac tgcggcgtca gctcgagtaa
    27901 ttcggtcatt cgcgccacca tatacggctg cgaaatcgtc tgcccctgac ctatcggcaa
    27961 ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt cacgcggcac
    28021 ggcggcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta attgatccag
    28081 aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt taaccagtct
    28141 gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt gatggagaca
    28201 tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt accgcccggc
    28261 gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac ctgatctgcc
    28321 ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa gggtaaatcc
    28381 ggaacattaa tattaagaat acgcccggtg cgcagcggct ctttacacag tgcgcgcaaa
    28441 attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag cgagacggca
    28501 agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc ggaataaata
    28561 acatcatccc ccagattcgg cccggcgtta attccggaca caacaatgtc cgggcgcgga
    28621 cgcatcagag cattcacgcc aagatagacg caatcggtcg gggttcccat ttgcacagca
    28681 atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag agaatttgaa
    28741 gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc acgcaaggct
    28801 ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag caatatgcgc
    28861 ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac tacccgccgg
    28921 aagccagaaa cggatttcta cggtgacgtc atcccaccaa ttccagctta attgttgcgg
    28981 atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta aagcttgtaa
    29041 ttcagtttct gcggcgacag ctgcctgttc gaatgccagc gcttcacgct gagttcccca
    29101 ttcaccactg cccggcaatg cggcggttat catcagctct ttatcgttga cgcgacgctg
    29161 taattccacc agttcttcgg tggttgcaac aaaccagcta ccacgtccgg ctaattgtag
    29221 cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag caacaatctg
    29281 attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc gatcgcgcac
    29341 cggagtattg gtttgcgccc agcgcagcgc gccctgcaag ttgctaccgc caatcccaaa
    29401 acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgatca gacgttgttc
    29461 aacgtcatcg cgattgctca cttcgcgcag aaccagggta aaggcgttac ctttcagcgc
    29521 ccctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc cttccaattg
    29581 aaaggcgctc agatcgggca tttccttgcc cggcacgcga gcgcataacc actgttctgt
    29641 aacagcatgt ttgtcttttt gcccagcaaa gctgacttca cgggcatgaa ttttcaggaa
    29701 tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga ttctaaccag
    29761 aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca caaagtcttc
    29821 cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag tgagattatc
    29881 aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc aatcccttcc
    29941 ccacgtccgg taaatccaag tttttccgta gtagtggctt tcacgttaac atcatccata
    30001 tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg caacatcttc
    30061 ggtgcctgag cgatgatagt gacatcgacg ttgccaaggg tataaccctt cgcctgaata
    30121 cgacgccagg cttcgcgtag cagctcgcgg ctatcggcac ctttaaatgc cggatcggta
    30181 tccgggaaca gtttgccgat atcccccagc gccgccgcgc caagcaatgc atcggtcaac
    30241 gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta aggaatgcgt
    30301 acgccaccaa tgataattgg gccttcaccg ccaaaggcgt gtacgtcaaa accgtgtcca
    30361 attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg gccagtgcca
    30421 aatcctccgg gcgcgtgact ttaatgttat ccgcacggcc ttcgaccaac tgaggatgga
    30481 atccgcaata ttccagcgcc gaggcttcgt cggtaatagc cgcgccttca tttagagcgc
    30541 gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc gcgtgccata
    30601 agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca cgtttcatag
    30661 tatcgcgcac tggtgcggct aggatccctc ccgtgcggct ggtttcgctc aacgccaaca
    30721 atcgcgcgag gtcatcctga tgcagacaag gacgagcggc gtcatgcacc aatacccact
    30781 gcgcgtcgcc agcggctttc aaacctgcca gcacggaatc ggcacgctca tcaccgccat
    30841 ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg ctatcgccag
    30901 gacttatggc aatgacgaca cgtttcaccc ggggatgcgc cagcagcgca tacaccgagt
    30961 gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc gtttgcattc
    31021 gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg gttgccatgt
    31081 taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc ttcgacgcgt
    31141 caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca ttacgcgcac
    31201 gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa agttgatcgt
    31261 ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatca ttgacgcggg
    31321 tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag accagaatag
    31381 ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat catcatccca
    31441 tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc gggcaaatgt
    31501 accacatttg tccattgtta cgtataccca gggcgtgcag aacataatct cattattagt
    31561 tacggtttga attatgaaca gaggagacaa gaaagtacaa attagcccag tagccacata
    31621 aacagtgcgc caaacataat gcctactgtc atcagggtga aaacaatact gtagcgtagc
    31681 tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcaacggg catcagcgcc
    31741 agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata aatcaacatc
    31801 ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg cagtgaccat
    31861 gtggtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt atggctatta
    31921 cgcatatttg atcctgttac tttgacgaac cgggcatgga aacccggtgg tgtctcagga
    31981 tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat ttgttactaa
    32041 ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg aatctattcc
    32101 cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagccttttg gatcgcgggc
    32161 ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt ctcctacgcg
    32221 ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca gcaccaccag
    32281 tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct ctttacgatc
    32341 ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattgtcgc catccagcag
    32401 atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga ccgttgattt
    32461 accggacccg gagaggccgg taaaccacag cactacacca cgatgaccgt ggtgtagctc
    32521 gcgttgttgc acagtgaccg gatggctatg ccaaacgacg ttttcgtcat gcagcgccat
    32581 tatttctccc ccagcaaatc gcgcgcgccc cagtgtggaa agtggcggcg aaccagggca
    32641 ttcaattcca gttcgaatgc actaaattca gatggcgcag cagttgcctg gctaactggc
    32701 tcatgcacca taccggcacc tacggtcaca ttgctcaggc gatcgataaa aatcagccca
    32761 ccggtaaccg ggttttgctg ataacgatct aacaccagtg gctcgtcaaa agtgagatcc
    32821 accaggccga tgccgttcag cggcaggttt tcaacttcgc gttgggtaag gttattaata
    32881 tcaacctgat aacgaatgcc atcaacacga gaacgcgtct tcttaccggc aattttgatg
    32941 tcgtaactct ggcccgggga aagcggctgt tccgccatcc ataccacatc caccgacgcg
    33001 ctctgcacag ctggtaacgc ttcgtctgcc gccagcagca gatcgccacg gctgatgtcg
    33061 atctcatccg tcagcaccag ggtgatagct tctccggcaa aggcttcttc gcgatcacca
    33121 tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac ttttacacgt
    33181 tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa atcgagattt
    33241 gggcggttaa cgtactgcac cgggaagcgc attggctggg catccaccac tcgctgaatc
    33301 tccacggttt ccagcacttc gagcagtgtc ggaccgctgt accacggcat actttcactt
    33361 tgcgaagcca cgttgtcacc ttccagtgcg gagagcggca caaagcggat atccagatta
    33421 cccggcagct gcccggcaaa ggtcagataa tcttcacgaa tacgggtgaa cgtctcttca
    33481 ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat ccccaacagt
    33541 gtggagataa aactgtgacg acgggtttga tcgagtacgc ctttacgggc atcgatcagt
    33601 aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgggtgta ctgctcgtgc
    33661 cctggagtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg gtaggccacg
    33721 tcaatggtga tgccctgttc acgctcagct tgcaggccgt ccaccagcag agccagatcc
    33781 agcttttcgc cctgggtgcc gtgacgctta ctgtcattat gcagcgatga gagctgatct
    33841 tcatagattt ggcgggtatc gtgcagcaga cgaccaatca gggtactttt gccgtcatcg
    33901 acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat catccaggct
    33961 tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt aaaaataccc
    34021 ctgacgtttt ttcagctcca tagaaccagc ttggtcgcgg tcaatcacgc gcccctgacg
    34081 ttcactggtg gtggaaacca gcatctcttc gatgatctcc ggcagcgttt gtgcatttga
    34141 ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca tccgtttttt
    34201 aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca acataccgtc
    34261 gcgttccaga accggacgtt ccgcagcgag atacagcggc acaatgtcga tattttccag
    34321 ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaaa cgcggatgct
    34381 ttcgcctttg ttaatctgcc cgttatagtt gtgccacagc tccgggcgct gatttttcgg
    34441 gtcccagcga tggaagcgat cacggaaaga gtagatacgc tctttagcac gggatttctc
    34501 ttcgtcgcgg cgcgcaccac cgaaggcggc atcaaaaccg tatttattca gcgcctgctt
    34561 caggccttcg gtcttcataa tatcggtatg tttcgcgctg ccgtgcacga atggattaat
    34621 ccccatcgcc acgccttccg ggttttgatg caccagcagc tcgcagccgt aggctttcgc
    34681 cgtacgatcg cggaactcat acatctcacg gaatttccag ccggtatcga catgcagcaa
    34741 cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca tgacgctgga
    34801 atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca cctcgcgaat
    34861 aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgtattt gatccataac
    34921 cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata gggagcgtaa
    34981 gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa cgatttggca
    35041 aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa gatttgaaat
    35101 tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat ggtatttaag
    35161 gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc ggcgtatgct
    35221 ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgccaat acacaggcgc
    35281 gacatattgc cactttcttt ccgggacgaa tgaccggaac acccgcagaa atgttatctg
    35341 ccgattatat tcggcaacag tttcagcaaa tgggttaccg cagtgatatt cgtacgttta
    35401 atagccgata tatttatacc gcccgcgata accgcaaaaa ctggcacaac gtgacgggaa
    35461 gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcattatt atggcgcatc
    35521 tggataccta tgccccgcag agcgacgcag atgcagatgc caatctcggc gggctgacgt
    35581 tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaactg gcagaacgcc
    35641 tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagtgga gaagaggaag
    35701 ggaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag aaaaatacgc
    35761 tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc aacagcggtg
    35821 taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca attgcgcgta
    35881 gtcatggaat tgccgcaacg accaatccgg gtttgaataa aaattatccg aaaggcactg
    35941 gatgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt tcggtggaag
    36001 cgacaaactg gaatcttggg aataaagatg gttatcagca acgcgcaaaa acagccgcat
    36061 tccctgcggg aaatagctgg catgacgtaa gactggataa tcagcaacat attgataaag
    36121 cacttcctgg aataatagaa cgtcgctgcc gtgacgttat gcggataatg ctaccgctgg
    36181 tgaaggagtt ggcgaaggcg tcttgatggg ttggaaaatg ggagctgggt gttctaccgc
    36241 aggggcgggg aattctaagt gatatccatc atcgcatcca gtgcgcccgg tttatccccg
    36301 ctgatgcggg gaacacagcg gcacgctgga ttgaacaaat ccctgggccg gtttatcccc
    36361 gctggcgcgg ggaacacaaa ccgaaacaca cgatcaatcc gaatatgagc ggtttatccc
    36421 cgctggcgcg gggaacactt tggtgacagt ttttgtcact gttttggtga cggtttatcc
    36481 ccgctggcgc ggggaacaca ctaagcatac atatctgttt ttaaacaaat ttattccaca
    36541 tcaacaatct accaactaaa ttcaaacatt tccttatttt taaagaacac ataacctatt
    36601 gattatcaac aggaagaaaa gaaaccaaac gtaacccatc caaatccacc ggaatacgtc
    36661 tgttttctcc ccaggtctga aattcaaaac ccgactcggt attggtcgcc caggccatca
    36721 ccacatttcc gcaaccagcc agttgggtaa tttgctgcca gatcatctcc cgaatacgtt
    36781 ttgatgtatc accaacatac acaccggcac gcacttccag tagccagatt gcgagccgtc
    36841 cacgtaagcg cggagggaca ttttctgtaa caaccacgac catgctcatc cgccgcgccc
    36901 ccggtgacca ctatcaccca gcgtttcagg ttcagggatg gcaggcggta acatatccgg
    36961 cgcgggttgt ggtggttcaa tttcacctgc agcaaggact ttctcaatta acggtattaa
    37021 tttgcccgtt aacttagtgc tacggaaaat atcgcgacag gctaatctga cttctttatc
    37081 aggttctgcg ggttgcctcg ctgctatttc aaatgccttt ggcacaaccg aatcaaattt
    37141 aatgatatcg gctatgtcat aaacaaatga aagcggtttg ccactatgaa taaatccaat
    37201 agcgggcgca tatcccgcgg ctaatactgc cgcttcagaa ataccgtaca gacatgatgt
    37261 ggcagcactg atgcagcgat tcacaacatc gcctttttcc cagtctttag gatcgtattt
    37321 gcgaccattc catttcacac catattgttt cgccagtaat gcataggtct ggcgaacgcg
    37381 ggatccctca attccccgta gctgatccac tgaacggcga gctggcggtg gctcacgaaa
    37441 acgtaattca tacattttgc gcaccacctt caggcgtaga tcttccgtta aagccagctt
    37501 tgcctggtag agtaatttat ctgcccgcgc ccctccgggt tgtccggaag agtaaacgcg
    37561 aacgcccgct tcaccgaccc agaccagcag tgttcccacc gtggcggcca gatgcaccgc
    37621 cgcgtgggaa actctcgttc ccggttcgag cataatgcag gcgaccgatc ccaccggaat
    37681 gtgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg ccgtccagta cgtcgatttg
    37741 accgtactgg aggaagatca tagaggtgcg atcttttaac gggatcggac tcagtggtac
    37801 aaacgtcaca cctctgctcc gggtttgatc agcatcagac cacagccgaa cgcgcggctt
    37861 ttgccatacc cctgactgag acgttgcaga aataacccgg gatcggtgac cgtaagcatc
    37921 cccgtataat ccacgctact gaactggatc agttgccggg agttttcccg ccgcagttgc
    37981 tgttgtcggt aggcatcaac cgaggtatcc agcagtgtaa aaccgcttct ctccccctgc
    38041 gctgccagcc agtccagcgc cgcctgttgt tgatgcaacc agacatcact tccttccgcc
    38101 tgccccctca cctgccgttt cgcctccatc agcagatcgt ggcgcttgcc cgctttacag
    38161 attgttggat tcgcccgcag gttgaaacaa agttgttgtc cggtacgcag ctcgggcaca
    38221 aatgaccggc attcgatggt gaacgtttca ctttccgccg ggcgttcctg cgacagtaca
    38281 aaaaagcgaa acgcgccctg gagttcttcc cgacgataaa gaaattgcct ttctttgccg
    38341 ccagggaaga gatcccacag ccactgatgc atcacatatt ccccgcgatc caccaaatgc
    38401 agcaactgcg caggcgaaag ctggccggta tgtaaggtta ttcttgagag gtacatggtt
    38461 cctccttgct gagccacggc ccctgattga tggtgcgttc cccaaacagc cactgctgac
    38521 gatttaaagg aacatcccgg cgacgtaata ttttgctcgc gaccaggccg tcgtgttccc
    38581 cttcccacca gcattcatcc tgaagtttcg ggagtgagac tttcagttcg cggaaactat
    38641 cctgatactg ttggtatgcg ttacgtaaga catcagacgc gttgccttcg agcagtaacg
    38701 gcgcaagtgg taacgccagc ggatgacttt ttcgccccag ataaagcgga aaaaccggat
    38761 gacgtaaacc gtcctgcaac tgttcaaggc tgtaaggcgc atcgggggtt gttgccaccg
    38821 ccaccatcca ccaggcatcg gtgtagtagt cgcgccggga gataatcgcg ctcagaagag
    38881 caggggcgct caactcttcg cgacggctga aataacgcgc tttacgcacc tcttttagca
    38941 tctggaccgt gtgataatcc cgtgcccagc gcgggttacg gctggcgcaa accaccagtg
    39001 aatagtggcg gttaaacgcg tttaatcgtt cggtatcatc acgccgaatc cctaccccgg
    39061 cagccagcag ccccagcaat gctgagcgcg aaggcagttc atgggtatga cgcacttcgc
    39121 cgggggcatc gacgccccag gatgccattg gcccatgaag ctgaaaaatc aaatattggc
    39181 tcattagccg ccccttacgc gcagataaag tccagcacgt ccttcatgct tccctgtttg
    39241 ttcatcacgt caaagcttgc gcattcggtc ttctgttcat agaccgtatt catattttcg
    39301 cgaagcgttg taatacgctg caccgccaca tctaactgcc gggtgccatt aatgggttca
    39361 tagaaagccg ccgccagaga acgtggttgt tcggtgcctt tttctgccag cgcccaggag
    39421 gcgtaggcac ggctggcaaa gctgttctgt ttgccggttg gggagacttt aagtgcggct
    39481 tccgtaaagg cgcgcaaggt ctgattagct aacgcttcgt caccgccgag gttttcgacc
    39541 agcagatctt tatcgatgca gatataggtg tagaacagcg cagaaccgaa tccggtttca
    39601 ccaagatgcc cggcaccggc atcttcagaa gcctggcgca aatcatcaac ggcggtgaaa
    39661 aaatcatctt cgacaatcgt ttcactgaca ccaaatgcat gcgcgacctg gcaggcggct
    39721 tcaacattaa attcgggttt attcgccagc atacgaccaa acatagcgat atctactgcc
    39781 atgcgatctt tacgtaacaa agcgagatct tcctcttttg gcgcgcgctt ttcttcggcc
    39841 agttgatggg ccagcgcttt tacggcgtca aattctgccg ggctgatatg gactaattgt
    39901 tcagtttcgg cgttagtgag cggatctttt ggttttttgt cgtttttagc tttcccaaga
    39961 taatccgcaa tttttgccgc ccattcgatg gcttttttct cttcgatgcc tttctcaatc
    40021 aggatagttg ccgcctcacg cgcaatacgc ccactgcgaa taccaatatg gcccgccagt
    40081 gcctgttcaa aaagtgcaga agtgcgccac gcacgtttca gactttgcga ggaaacgcgc
    40141 agtcgcgttg ctccacccag gaccacggtt ttcggcgctc cggtgtcatc acggttaagg
    40201 ttggctgcag ggtaagcggt taacaaatga agctgaataa atgtcgtcat gagatagtcc
    40261 tttattgatt ttgttcgtta tcgacgtcgc cagcctggta atattccagc gcccagcgga
    40321 tacggataaa ttcggttggc cgctgatgac ggcggtgatg attgagcaga tcgtcgctct
    40381 cctggcacca gcggaagaca ccctctgcca gagagtcaag attaacggaa ccgtttaata
    40441 atctgactac gcggcgtaac tggcgcagta attcatccgg tgtttttact gccgacaggc
    40501 gagtaaaacg cccctttgac attaccgccg ccagctgcgc agcaaaaggc agccgttcat
    40561 caatggcttt aacattagcg ctaagtgcgg ctatcagcgc cagtgcggta atacgccatt
    40621 caggttcatc ctgccacttt atttgtctgt tcttcagaaa caggcgaaat ccatccgtca
    40681 gacaaacatc attaaccgtc gtactacgcc gcagactggc acgttcgccg cgtttctcct
    40741 gcaattcctc atgccatttg cgcagcgtgg ctttgtgctc ctcttttaca atactcattc
    40801 agcagcctcc tgctttttct ccctggcggc tttagcactt tgcttctccg ccgatgttgt
    40861 aaaatatttc ttgcgcgcgg tcatgacgcg ttccaaatca acgggctcat aaggattggt
    40921 gaatacacgc tcgtcaaaat cctgacgtgc gaataaccaa atttcctttt gccatttgcc
    40981 gagtaattca tccgcatcct gaccttcttc aatttggcgc actaacctca ggaagcgatg
    41041 ctgagttttg ttccagaagt cgatatccac aaaactgaaa tcaccccttg caccttttgg
    41101 atcggagaac catgcttctt tcaatgcact ccgtaacaga ctcagaatcc gtgaagccgt
    41161 ttgcgcagcc agccgcagct tcggtatctg gccttctttt ttattgagca gcagcgggaa
    41221 atggtgttcg taccaacagc gcgctttcat gttgtcgaaa tcataaccaa atccccacag
    41281 gcccactttt gcctgtttca gactgctggc attaaagagt ttcaccacca gcgcgggaag
    41341 ttccgtattg ttttctgact tacccgtttc gataaggcct aaccagtcgc gccagattaa
    41401 accgcccggt tgtggtttaa cggagtaaaa ctcaccgccc tctttaagtg gtacacggta
    41461 aggcgttaag ggatgctgcc acatggcata attcgcaccg taatttttgg tagtcatcaa
    41521 actcagaagc gcgtcactct gctcaccgca aatatcgcag ttgccgactg tcgtggtatt
    41581 aaaatcaata cgaatacgcc gcggcattcc ccagtacgcc tggagtttat tgacctgatc
    41641 atcggttacc accgcaccgg ccagttcgct ggtacgcgtc gggccaagcc aggggaaaac
    41701 cagatcgtca aattttttgg gtagcggtaa gtcggcttca tcctgcggca tcacgttgag
    41761 ccacagtttg cgccacaagg gggcttgttg attgccctga tactcctgca attcaatcag
    41821 agtcgtcatc ggcccaccgc cgcgtaaacc ggtgcgatag cctttgccac ctgacggcgc
    41881 atttaactgt agggagaaca gagctaacgc agaacaatga gagcatacgt gttcagtcac
    41941 gccacgctta ataaagtggt ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg
    42001 cagtagcgaa gcgacctgaa ctttatcgcc catgagcacc tcgaaatcct gcataaatga
    42061 aggtgaatct gggccaaact ggaaagggtg ttctaatgac agcaatgctt cccgtagctt
    42121 ttcagcttcc agcccgtctt cccagatatc atcccaacga cgataatctt ttggcgcgaa
    42181 actgctttgt agtaacccca gcaaaaactg ccatgccgcc ccctggagat ctgcccgcgg
    42241 cgcagcgata tcgacaacat tttcatccgc cagatcgact ggcgccagct tgcctgttgt
    42301 tccgtcttta aaacgaacgg gcaaccacgg ggttgtcaga agtgaaaacg agttcatatg
    42361 tatcctctcg taaaaaagac catccctggt cggttgtcca tcattccatc cttttcggtg
    42421 tatcaactat tcagagagtc ctgtcgcagg tatctcaatc aaccttgcca atcaatccct
    42481 ccctggccga atacccgcaa ctttcgtcat cagtgactaa aatcacgttt aaaatcagga
    42541 atgctgctcg caaaataaaa acgcccccat cattactgac agaggcgttt aatttatccg
    42601 gcaatattta cccttcgtgt aacccacatt ccctttttaa gccaaagaaa cgcgtttctt
    42661 cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc gataaatatc
    42721 cttcgtccca taatgggtga tatttcaggc cattcttttg cagatactga tatatcgttc
    42781 ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg
    42841 gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag gtttgcgcgt
    42901 taagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa
    42961 tgccttcaac gccctgttcc cacagttttc cgtagcgtgc ttcctgccag gctgcgcttt
    43021 cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc
    43081 ggtaggtttc cgggaacaag taacccgtat cggtgaggat caccggaata tccgggcgaa
    43141 tttgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat
    43201 attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc agtttttcca
    43261 gttgggcgtt agtttccgcc agcgccagaa tgcgatctac cttcggcagt tcgttcaggg
    43321 cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat gcgacgctca
    43381 cgcgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
    43441 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
    43501 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
    43561 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
    43621 aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat cgcgcgacca
    43681 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
    43741 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc ttccgccatc
    43801 gccagcgggc aagtcgggaa cgagacgcag gccattgagt tttcacgctg cggcgtgacg
    43861 gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc gctttccggc
    43921 acaccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt gtggatcttc
    43981 gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat gcgaccattt
    44041 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatct
    44101 ccgcgtccgg tgaactcata cggacggatc ggttcaaatt tgatccccgc gcgacgctcc
    44161 acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt tttggcattt
    44221 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc cgccactgcc
    44281 agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta ggttttcttg
    44341 ttgccgtgtt cgatagaaag tccaccgccc accaacaggt taaagcccac cagcttgccg
    44401 ttttcggcga ttgccacgaa gttcatgtcg ttggcgtgca gatcgatatc gttctgtggc
    44461 gggatcacta ccgtggtttt gaatttacgc ggcaggtagg tctggccgag gatcggttct
    44521 tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc gcgggtacga
    44581 ggcagcagat gttcagagat tttcttcgcc cactcgtacg cttctgcgtg cagctgcgac
    44641 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcggt cgccagcgca
    44701 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
    44761 tggaactgga atgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
    44821 gcaaatttat cgatcgcctg ccactgtttg gtggtgataa ctccacccgg cagacggcag
    44881 cgcaacagca tcgcatggcg cggctccagc ttctgttcag cacgttcggc gcggatgtcg
    44941 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc gcccttaaag
    45001 ccgccggtca ggccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
    45061 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
    45121 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
    45181 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
    45241 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
    45301 tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacgtaa actttttctt
    45361 tttgatcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg acgtaacgct
    45421 gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag aacagccagt
    45481 ttttacccgg cgcttcgtca gcggcgcgct gctgcataaa ggcgcggaac ggcgcgatgc
    45541 cggtgcctgg gccaatcata atcaccgggg tttccgggtt agcgggcagg cggaagttat
    45601 cgttatgttc gataaatacg cgaacttcgc cctcttcttc cacgcggtcc gcgaggaagc
    45661 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga
    45721 cgtgtacttc gttctcgact tcctcctgcg aggaggcgat ggaatacaga cgcggcgtca
    45781 gcgggcgcag cagattaatc agcgcttcgg cgtccagttg cgccggagag aagcgcacca
    45841 tgtcgacaat cggcgtagtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
    45901 gcaacagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatattg gcggtgttga
    45961 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
    46021 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccggat
    46081 cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggccc gagtcaccta
    46141 agtcaatttc gatatggcga acgtcttttt cagagttacg cccggtaatt ttctggttaa
    46201 ccgaaaggct cgctaccagc ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta
    46261 ccacgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
    46321 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg gcgtcgacac
    46381 ggtcgagcag gcgttcaccg cccagttccg ccagcttgct gtcgaaatct ttcccggact
    46441 ggcagaaaaa ttcataagaa ctatcgccga ggctaaacac ggcaaaagcg gtattttcca
    46501 gcttcggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
    46561 ccccttctcc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg atttgtttga
    46621 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgtcaat aaatcatcgc
    46681 gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga agcagagata atagttatac
    46741 ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc tgattgagca
    46801 cgccccagaa atagccagaa acccaggcaa gctgggtggg agttaaatcg gtcgtggccg
    46861 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
    46921 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
    46981 aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
    47041 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
    47101 taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt
    47161 gcgcaacaaa gtcgttttag ataatgcgaa aaaagagcct ttccggtact ctacggcggt
    47221 tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
    47281 agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca
    47341 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
    47401 cgatttcgct gaactaaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta
    47461 tctcaatgat attccaggtc tggaaaaccc aactagcgaa gttttagcaa aatggatttg
    47521 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
    47581 cgctggttgt atctatcgcg gcgaatgata agagtgtggc ggcggtcaat ttcccttaag
    47641 taacgctatg ttagggtgtt gtgttcagga tatctggggc atgacatgga agacgactgc
    47701 gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga
    47761 gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc
    47821 ggcgggcggt tatataccca tgcactcgcg gaactcctcc ctcaatttca tctgaccgcg
    47881 cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgccgga ttgcgcaacg
    47941 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat
    48001 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccctgg tgcgacggtg
    48061 gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga cgatattctc
    48121 cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg
    48181 ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg
    48241 gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg
    48301 ttcagcggcg gaatctgtga tgacttaccc ggcgcgcatt tctttatact aatcaacaaa
    48361 cgctctcgtt agggattgtt tgcccactct cttcccttac gcaaagtcgt gttccggcaa
    48421 gcgagctgct tgctcgcttt aaaacgcatc cggcagtgcg cccgcttatc aaaaacacgg
    48481 aatcactgga gtatggtgcg catctggtgc cagaaggtgg cttgcacagt atgccggtac
    48541 aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt gcgcagttgc gtcaataccg
    48601 gaatttctgt gcgcggcatg gatacggcgc taactggcgc gcaggcggcg gcacaaacac
    48661 tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt tccgctttat catcacaacg
    48721 tagagcgcag cctgctgtgg gatgttctac agcgttatca gcatgttccg gtgcttttgc
    48781 aacgcccggg atggtaccgt acgtggcctg cgttaatgca ggatatttcc cgcgatttat
    48841 gggatcaggg tgataaacct gttccaccgc tgcgccagtt actctggcgt catttacgtc
    48901 gtcatggctt gtggaatctg gcgggcgatg ttatcaggag tgttcgatgt ctgtagcccg
    48961 taatctctgg cgcgctgctg atgcgccgca cattgttccg gctgactccg ttgagcgtca
    49021 gatggtacaa cggttgatta acgcctgtcc ggtaggtctt ttttcgctca caccggaagg
    49081 tgacttacgc gttgattatc acggctgcct ggagtgtggc acctgccgtt tgctgtgcga
    49141 cgaatccaca ctacaacagt ggcgctatcc gccttccgga ttcggcatca cctaccgatt
    49201 tggataaata ataaggataa tttatgcccc tcttacacct gctccgccag aatccggtga
    49261 ttgctgccgt taaagataat gccagcctgc aactggccat cgattctgaa tgccagttta
    49321 tttccgtctt gtacggcaat atctgcacca ttagtcaaat cgttaaaaag attaagaacg
    49381 ctgggaaata tgcttttatt catgttgatt tgctggaagg cgcgtcaaat aaagaagtgg
    49441 tgattcagtt tttgaaactg gtgaccgagg cggatggaat tatcagcacc aaagcatcga
    49501 tgctaaaagc ggcaagagcg gaaggttttt tctgtattca tcgcctgttt attgttgatt
    49561 cgatttcgtt tcacaacatt gataagcaag ttgcgcaatc gaatccggat tgtattgaga
    49621 tcctgccagg ctgcatgccc aaagtgctgg gctgggtgac ggaaaaaatc cgccaaccgc
    49681 tgattgctgg cgggctggta tgcgatgaag aagatgcgcg taatgcgatt aacgcgggtg
    49741 tcgtggcgct ttccaccacg aataccggag tctggacgtt agcgaaaaaa ttgctttgac
    49801 gggataatcg taaccaattg aatttggttt gatttgtagg ccgcacgcca catccgacat
    49861 tcagcgcctg atgcgacgct taacacgtct tatcaggcct acaggtcccc agtccagaac
    49921 cgtaggcagg atcatccggc agcacgttat tggcattctg cgtcaatgtt tgtcaccagt
    49981 gcctccagca tcactttcca gtcatcaacc acgccaacat ctgcctgtga aaataccgct
    50041 gcgctggcgt catgattaat cgccaccaca aacttgctat tgcgtacgcc cgccattaac
    50101 gctgctgcgc cagaagcgcc aacaacaata cacacctcag gtgccagcag atgtccggaa
    50161 ataccaatca ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg actgtagccc
    50221 acttcggctc ccagctttta tgccagcata gcgatttcct ggttatctgc ctctccgccc
    50281 tgccccacca ccaacacccg tctggcctca gctaatgggt cgcgggtaac atttttcagg
    50341 ttctctatgc taaccagcca gtccggaagc gcacctggga ctatgttcag ttgctgcata
    50401 ccagagggca acgtggcatt tttatctgct cccgcctggc gcgccaggga aagacatagc
    50461 gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga tttgcgcacg
    50521 cttacgattg gaatctctaa cgaagtcacc tggcagatgc ttgcaccatg taaacgccag
    50581 gccagccgag tcgacagttc atcgccaaac gttcccggcg gaaagagaac aacgttcgca
    50641 ggcgttcgct gccactgttc caccagcgca tccagcacct gttccaccac tacgggctgt
    50701 ggttctattt gccagtgcgc cagggtacaa ccgctgaaat cctgcgccgc cagccagccc
    50761 gctaatgcgg cattctcctg atttattgtc acaattgcga tattcatggc tgcatccttt
    50821 ggcgtaaata gtcctgccat agcttttgcg ctttttctgc taccgtttgc ccatcaatca
    50881 gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag acactgcatc gctggcaatt
    50941 cggcagcaac agttttacgg ataatttccg ctttccccgc agccattcgc tggcgcattc
    51001 ccggtactgg caaggccact tcaccgcact gacgcacggc aatcaccgca ggcaagcgaa
    51061 tccgacaaca gcgcagccca tgttcagtac gctgttcgag ggtgataaac ggcgcgtcga
    51121 gagtgaaacg ctccacctgg gtgaagcaag gccagcccag catttccgcc agcaaaaatg
    51181 gcgtttgccc attctgccct tcgctgcttt ggcagccggt aattatcaga tccagcggat
    51241 tctgacgctg ccattcggca atatggcgag cgacaaattc cgacgcaaag cgcagatctg
    51301 ccgccgtctc cagcaataca gcttcctcaa acccaagagc catgagatag cgcagccagt
    51361 gcaacgcccg ttcatccccc atgctcaacg cggttaaaga catcggcgta ccgttttttc
    51421 tctgcgccaa cagcagcgcg gcggcagcct gttcatcagc accgagtaaa cttcgcagta
    51481 gcgaaatatc cggtccgcta ttaccatgag ccgccgcctg ccactctttt tccgccagca
    51541 ttccggcatc cggttcggct ttaaacgcta acagaatgtt cattcgcacc tcctacattt
    51601 cccgccgcca ccagcgggag tgctttggtt tctggtgccc atagccaggt tacaacaaaa
    51661 ccagccaaca acactgccgc caggagcaat aaggtgactt gcattcccca ctgcgccagc
    51721 acccacggca gcaggccagt acttaccgcc gcccccaacc gactcatggc ggtggcaaaa
    51781 ccgacgccca gcgagcgaat gtcagtagga aaactttccg caggcaaaat gcccaccaga
    51841 ttactgactg ccgaaatggt ggtgctgaag agaacaaaag cagcagcgtt aatgaactgc
    51901 cggaaggcaa acaggccatc actaccagcg ttgccgccag cagcaaaaaa cttcccagca
    51961 aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg cccacaatta
    52021 acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagtccaata gtctgggcga
    52081 tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca aagaagacgc
    52141 tgttaaacgc cgtgcgccgc cagtagcgcg aagagaacaa ggttttgatg tgtttatggg
    52201 tcgccgttgc cacttcatcg cccagtaaaa catggggacc aaaatagcga tgcacgatag
    52261 cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggcgattct ggcgttcccc
    52321 agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagcagc cagcgccagg
    52381 cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccaacaca tagcctacgg
    52441 tccacaccac gctgaatgcg cccagcaaaa caccgcgatg gcggcgcggg gaaaattcag
    52501 ccagcaaggt gtgacctact gagtaatcgc ctcccagacc aatgccgatc aaaatgcgca
    52561 gtccaataag atgctctggc gtggtggcaa aaaattgcaa gaacgaagcg agcgtaatca
    52621 gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc cacccaagaa
    52681 ccaggctgcc aagaaacaaa ccaaggtgcg ccgagccgcc gatcattccc gccataaacg
    52741 gcgtctgttg catggcgggc gtaagttgaa taatggcgta accaatgacg ccgagaacat
    52801 aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat tcgacagtga aaacggttaa
    52861 gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca ttgtccggta
    52921 ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccatcatc gcggaacgct
    52981 gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg ccattaggat
    53041 cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc agcttgctcc
    53101 agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccaccg aggcgaatgg
    53161 tttcttcgca aatgattttg ttgagcggat tgtggtactt gtcgatttcc tcttccggct
    53221 tacagttgac aacgttgtag tcgtagacga agtacatgtt ggtgccgttc tgatagctat
    53281 gagaggagtg accgcccagc atggtgatgt cgtcggcgtg cgggaactcg gtacgaatac
    53341 ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac acttcggtag
    53401 taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact ttatccggtc
    53461 cccagttcag gttgttaaac caggtttcga tcagcttgct gtccacgcgt tggcattgcg
    53521 ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg cgagggttac
    53581 cttcagccat aaagatcagc acgcattttc cgtcggcaaa atgggtgaag tgttgggtgc
    53641 catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc atgatttcac
    53701 gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag aggttgtttt
    53761 ccggggtgaa tttaaagatt ttcactgtta cttcagtgat atagcacaat gcaccttcgt
    53821 tgccgatgat gatgtgacga atgtccgggc cagccgcgcg gcgtggcacg tttttaatgc
    53881 gtgtgacggt gccatctgcc aacactgcct ccagaccaac gaccatatct tcgattgcgc
    53941 cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc atctgcgcca
    54001 gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac gcgttttcca
    54061 gcacctccag cggaacacca cattgcgccg tcgcctgcat attctcaata tcaatattaa
    54121 tgatttgatt catggcggaa ccgtcgagca ccaccgagtt ttctacaaca gtttccagcc
    54181 caccttcggt ggcggaagca ccggtacgcg gcacaccgtt aattttgtgc gcattcataa
    54241 aattcagcac acgggatact tgctctgtgg aaccgagttt tacgaccgct gccggaatcg
    54301 gcaaagtata aatgccatga atatccggaa atttacgaaa acggtcaata ctgttcttct
    54361 ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt agctggtcga
    54421 caatcgctgc gcgagataaa gacatagtaa ttccttcctg ataataaaaa tggaataatc
    54481 attaataaga gaaaacagaa accaccgtta tgggttagcg cactaaataa ccgccatcga
    54541 ccactaataa atgcccgttg acataattcg atgcctgact tgcgagaaac acggctgcgc
    54601 ccattaaatc ctgagtatcg ccccaacggt ttgccggaat atgatcaaga acgcgctgat
    54661 tggtttctgg attactgcgt gtcgccagcg taatgtctgt tgcataatag ccaggggcga
    54721 taccatttac ctgaatatta tattgaccta gttcatcaca ataagctttg gtgaatccgg
    54781 caagagcatg tttagtagca gaatatgcag gtgaccattg tccacctaag taagagaaca
    54841 atgaacagat attaatgatt ttaccgcttt tctgcgggat cataattttt gccgcttcgt
    54901 agcttaactc gaatgcggcg gtcaggttca catcaatcat cgggtcccag tcggcacgac
    54961 cgaagtccag caccttattc agcttacaaa taccggcatt gttaaccaga atatcaactg
    55021 taccgaaacg ctcacagcag gcagcgataa tcttctgcgg cgcgccttct gcggtgatat
    55081 ccacctgcat gaagtccacc tcaacaccct gattttcaat catttccttt gtttcgccgt
    55141 tatctttgac aaaacttgga ataaagacat ttgcgcctgc tttggccaac gccatggcaa
    55201 atgcctggcc taaaccgcta ttcccgccgg taacaattgc ggttttacct ttcagggaga
    55261 aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc aatttccgta
    55321 aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt ctcattatct
    55381 ctggtaactg gagataattt ttagcataag gacataactg ctaatgtgat
//
LOCUS       DAZGXS010000014        54886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGXS010000014 DAZGXS010000000
VERSION     DAZGXS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            309..395
                     /gene="leuL"
                     /locus_tag="ACHKG5_001007"
     CDS             309..395
                     /gene="leuL"
                     /locus_tag="ACHKG5_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0867155.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            488..2059
                     /gene="leuA"
                     /locus_tag="ACHKG5_001008"
     CDS             488..2059
                     /gene="leuA"
                     /locus_tag="ACHKG5_001008"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0867156.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2059..3150
                     /gene="leuB"
                     /locus_tag="ACHKG5_001009"
     CDS             2059..3150
                     /gene="leuB"
                     /locus_tag="ACHKG5_001009"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0867157.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3153..4553
                     /gene="leuC"
                     /locus_tag="ACHKG5_001010"
     CDS             3153..4553
                     /gene="leuC"
                     /locus_tag="ACHKG5_001010"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0867158.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4564..5169
                     /gene="leuD"
                     /locus_tag="ACHKG5_001011"
     CDS             4564..5169
                     /gene="leuD"
                     /locus_tag="ACHKG5_001011"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0867159.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5277..5408)
                     /gene="sgrT"
                     /locus_tag="ACHKG5_001012"
     CDS             complement(5277..5408)
                     /gene="sgrT"
                     /locus_tag="ACHKG5_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0867160.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5497..7155
                     /gene="sgrR"
                     /locus_tag="ACHKG5_001013"
     CDS             5497..7155
                     /gene="sgrR"
                     /locus_tag="ACHKG5_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0867161.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7384..7953)
                     /gene="espY2"
                     /locus_tag="ACHKG5_001014"
     CDS             complement(7384..7953)
                     /gene="espY2"
                     /locus_tag="ACHKG5_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFP0867162.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8195..8294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8325..9308
                     /gene="thiB"
                     /locus_tag="ACHKG5_001015"
     CDS             8325..9308
                     /gene="thiB"
                     /locus_tag="ACHKG5_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0867163.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9284..10894
                     /gene="thiP"
                     /locus_tag="ACHKG5_001016"
     CDS             9284..10894
                     /gene="thiP"
                     /locus_tag="ACHKG5_001016"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0867164.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10878..11576
                     /gene="thiQ"
                     /locus_tag="ACHKG5_001017"
     CDS             10878..11576
                     /gene="thiQ"
                     /locus_tag="ACHKG5_001017"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0867165.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11663..12427)
                     /gene="yabI"
                     /locus_tag="ACHKG5_001018"
     CDS             complement(11663..12427)
                     /gene="yabI"
                     /locus_tag="ACHKG5_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0867166.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12513..13391)
                     /gene="araC"
                     /locus_tag="ACHKG5_001019"
     CDS             complement(12513..13391)
                     /gene="araC"
                     /locus_tag="ACHKG5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0867167.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13730..15430
                     /gene="araB"
                     /locus_tag="ACHKG5_001020"
     CDS             13730..15430
                     /gene="araB"
                     /locus_tag="ACHKG5_001020"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0867168.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15441..16943
                     /gene="araA"
                     /locus_tag="ACHKG5_001021"
     CDS             15441..16943
                     /gene="araA"
                     /locus_tag="ACHKG5_001021"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0867169.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17143..17838
                     /gene="araD"
                     /locus_tag="ACHKG5_001022"
     CDS             17143..17838
                     /gene="araD"
                     /locus_tag="ACHKG5_001022"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0867170.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17913..20264
                     /gene="polB"
                     /locus_tag="ACHKG5_001023"
     CDS             17913..20264
                     /gene="polB"
                     /locus_tag="ACHKG5_001023"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0867171.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20428..23334
                     /gene="rapA"
                     /locus_tag="ACHKG5_001024"
     CDS             20428..23334
                     /gene="rapA"
                     /locus_tag="ACHKG5_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0867172.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23346..24005
                     /gene="rluA"
                     /locus_tag="ACHKG5_001025"
     CDS             23346..24005
                     /gene="rluA"
                     /locus_tag="ACHKG5_001025"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0867173.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24218..24396
                     /locus_tag="ACHKG5_001026"
                     /pseudo
     CDS             24218..24396
                     /locus_tag="ACHKG5_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24423..25160)
                     /gene="espY1"
                     /locus_tag="ACHKG5_001027"
     CDS             complement(24423..25160)
                     /gene="espY1"
                     /locus_tag="ACHKG5_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP0867174.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATLDGATHLYDEVIIINKI
                     TPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(25455..26270)
                     /gene="djlA"
                     /locus_tag="ACHKG5_001028"
     CDS             complement(25455..26270)
                     /gene="djlA"
                     /locus_tag="ACHKG5_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0867175.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26525..28879
                     /gene="lptD"
                     /locus_tag="ACHKG5_001029"
     CDS             26525..28879
                     /gene="lptD"
                     /locus_tag="ACHKG5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0867176.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28932..30218
                     /gene="surA"
                     /locus_tag="ACHKG5_001030"
     CDS             28932..30218
                     /gene="surA"
                     /locus_tag="ACHKG5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0867177.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30218..31207
                     /gene="pdxA"
                     /locus_tag="ACHKG5_001031"
     CDS             30218..31207
                     /gene="pdxA"
                     /locus_tag="ACHKG5_001031"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0867178.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31204..32025
                     /gene="rsmA"
                     /locus_tag="ACHKG5_001032"
     CDS             31204..32025
                     /gene="rsmA"
                     /locus_tag="ACHKG5_001032"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0867179.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            32028..32405
                     /gene="apaG"
                     /locus_tag="ACHKG5_001033"
     CDS             32028..32405
                     /gene="apaG"
                     /locus_tag="ACHKG5_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0867180.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32412..33260
                     /gene="apaH"
                     /locus_tag="ACHKG5_001034"
     CDS             32412..33260
                     /gene="apaH"
                     /locus_tag="ACHKG5_001034"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0867181.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLLYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33257..33571)
                     /locus_tag="ACHKG5_001035"
     CDS             complement(33257..33571)
                     /locus_tag="ACHKG5_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP0867182.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33574..33807)
                     /locus_tag="ACHKG5_001036"
     CDS             complement(33574..33807)
                     /locus_tag="ACHKG5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP0867183.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33893..34372)
                     /gene="folA"
                     /locus_tag="ACHKG5_001037"
     CDS             complement(33893..34372)
                     /gene="folA"
                     /locus_tag="ACHKG5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0867184.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34564..36426)
                     /gene="kefC"
                     /locus_tag="ACHKG5_001038"
     CDS             complement(34564..36426)
                     /gene="kefC"
                     /locus_tag="ACHKG5_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0867185.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36419..36949)
                     /gene="kefF"
                     /locus_tag="ACHKG5_001039"
     CDS             complement(36419..36949)
                     /gene="kefF"
                     /locus_tag="ACHKG5_001039"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0867186.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37057..38388)
                     /gene="yaaU"
                     /locus_tag="ACHKG5_001040"
     CDS             complement(37057..38388)
                     /gene="yaaU"
                     /locus_tag="ACHKG5_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867187.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38446..38733)
                     /gene="fixX"
                     /locus_tag="ACHKG5_001041"
     CDS             complement(38446..38733)
                     /gene="fixX"
                     /locus_tag="ACHKG5_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0867188.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38730..40016)
                     /gene="fixC"
                     /locus_tag="ACHKG5_001042"
     CDS             complement(38730..40016)
                     /gene="fixC"
                     /locus_tag="ACHKG5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0867189.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40067..41008)
                     /gene="fixB"
                     /locus_tag="ACHKG5_001043"
     CDS             complement(40067..41008)
                     /gene="fixB"
                     /locus_tag="ACHKG5_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0867190.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41023..41793)
                     /gene="fixA"
                     /locus_tag="ACHKG5_001044"
     CDS             complement(41023..41793)
                     /gene="fixA"
                     /locus_tag="ACHKG5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0867191.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            42267..43781
                     /gene="caiT"
                     /locus_tag="ACHKG5_001045"
     CDS             42267..43781
                     /gene="caiT"
                     /locus_tag="ACHKG5_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0867192.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43812..44954
                     /gene="caiA"
                     /locus_tag="ACHKG5_001046"
     CDS             43812..44954
                     /gene="caiA"
                     /locus_tag="ACHKG5_001046"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0867193.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            45083..46300
                     /gene="caiB"
                     /locus_tag="ACHKG5_001047"
     CDS             45083..46300
                     /gene="caiB"
                     /locus_tag="ACHKG5_001047"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0867194.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46374..47927
                     /gene="caiC"
                     /locus_tag="ACHKG5_001048"
     CDS             46374..47927
                     /gene="caiC"
                     /locus_tag="ACHKG5_001048"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0867195.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            48036..48821
                     /gene="caiD"
                     /locus_tag="ACHKG5_001049"
     CDS             48036..48821
                     /gene="caiD"
                     /locus_tag="ACHKG5_001049"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0867196.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48827..49417
                     /gene="caiE"
                     /locus_tag="ACHKG5_001050"
     CDS             48827..49417
                     /gene="caiE"
                     /locus_tag="ACHKG5_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0867197.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49536..49931)
                     /gene="caiF"
                     /locus_tag="ACHKG5_001051"
     CDS             complement(49536..49931)
                     /gene="caiF"
                     /locus_tag="ACHKG5_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0867198.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(50192..53413)
                     /gene="carB"
                     /locus_tag="ACHKG5_001052"
     CDS             complement(50192..53413)
                     /gene="carB"
                     /locus_tag="ACHKG5_001052"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0867199.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53431..54579)
                     /gene="carA"
                     /locus_tag="ACHKG5_001053"
     CDS             complement(53431..54579)
                     /gene="carA"
                     /locus_tag="ACHKG5_001053"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0867200.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13127 a        14782 c        14310 g        12667 t
ORIGIN      
        1 tatcaaaaaa tccgcaaaac atataattca atacaaatca tcaggatagg ttttgcaacg
       61 cgtgcatttt gtcccctttt tcctcgtgga ttagatgcaa aaatttattc tgaaatatgt
      121 caaccgatga aaagcatcgg tagttaagca gaaattaata tcgcttgttt taaccacagc
      181 agcacaatta gctaatctta cggatgcaga actcatgctg gcgggacgtt tttattgcgt
      241 cagggttgac atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag
      301 ctgatttaat gactcacatc gttcgcttta tcggtctact actactaaac gcatcttctt
      361 tgcgcggtag acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaaac
      421 ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag agacaaggac
      481 ccaaaccatg agccagcaag tcattatttt cgataccaca ttgcgcgacg gtgaacaggc
      541 gttacaggca agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat
      601 gggtgttgac gtgatggaag tcgggttccc cgtctcttcg ccgggtgatt ttgaatcagt
      661 gcaaaccatc gctcgccagg ttaaaaacag ccgtgtatgc gcgttagctc gctgcgtgga
      721 gaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc gtattcatac
      781 ctttattgcc acttcgccaa tgcacatcgc caccaagctg cgcagcacgc tggacgaggt
      841 gatcgaacgc gctatctata tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt
      901 ttcttgcgaa gatgccgggc gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc
      961 gattaatgcc ggtgccacca ccatcaacat tccggacacc gtgggctaca ccatgccgtt
     1021 tgagttcgcc ggaatcatta gcggcctgta tgaacgcgtg cctaacatcg acaaagccat
     1081 tatttccgta catacccacg acgatttggg cctggcggtc ggcaactcac tggcggcggt
     1141 acatgccggt gcacgccagg tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa
     1201 ctgttccctg gaagaagtca tcatggcgat taaagttcgt aaggatattc tcaacgtcca
     1261 caccgccatt aatcaccagg agatatggcg caccagccag ttagttagcc agatttgtaa
     1321 tatgccgatc ccggcaaaca aagccattgt tggcagcggc gcattcgcac actcctccgg
     1381 tatccaccag gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga caccagaatc
     1441 tattggtctg aaccaaatcc agctgaatct gacctctcgt tcggggcgtg cggcggtgaa
     1501 acatcgcatg gatgagatgg ggtataaaga aagtgaatat aatttagaca atttgtacga
     1561 cgccttcctc aagctggcgg acaaaaaagg tcaggtgttt gattacgatc tggaggcgct
     1621 ggccttcatc ggtaagcagc aagaagagcc ggagcatttc cgtctggatt acttcagcgt
     1681 gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa ctggcctgcg gcgaagaagt
     1741 caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg cgattaaccg
     1801 catcactgac tataacgtcg aactggtgaa atacagcctg accgccaaag gtcacggtaa
     1861 agatgcgctg ggtcaggtgg atattgtcgc caactacaac ggtcgccgct tccacggcgt
     1921 cggcctggcc accgatattg tcgagtcctc cgccaaagcc atggtgcacg tacttaacaa
     1981 tatctggcgt gccacagaag tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa
     2041 caacaaggaa accgtgtgat gtcgaagaat taccatattg ccgtattgcc gggggatggc
     2101 attggtccgg aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt
     2161 gcgatgcgca tcactaccag ccattacgat gtaggcggcg cagccattga taaccacggg
     2221 caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc
     2281 tcggtaggcg gcccgaaatg ggaacattta ccaccggacc agcaaccaga acgcggcgcg
     2341 ctgttgcctt tgcgtaagca cttcaaatta ttcagcaacc tgcgtccggc aaaactgtat
     2401 caggggctgg aagcattctg tccgctgcgt gcagacattg ccgcaaacgg cttcgacatc
     2461 ctgtgcgtgc gcgaactgac cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt
     2521 agcggacaat atgaaaaagc gtttgatacc gaggtgtatc accgttttga gatcgagcgt
     2581 attgcccgca tcgcgtttga atctgctcgt aaacgtcgcc ataaagtgac gtcgatcgat
     2641 aaagccaacg tgctgcaatc ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg
     2701 gaatacccgg atatcgaact ggcgcatatg tacatcgaca acgccaccat gcagctgatt
     2761 aaagatccat cacagtttga cgttctgctg tgctccaacc tgtttggcga cattctgtct
     2821 gacgagtgcg caatgatcac tggctcgatg gggatgttgc cttccgccag cctgaacgag
     2881 caaggttttg gactgtatga accggcgggc ggctcggcac cagatatcgc aggtaaaaac
     2941 atcgccaacc cgattgcgca aattctgtcg ctggcactgc tgctgcgtta cagcctggat
     3001 gccgatgatg cggcttccgc cattgaacgc gccattaacc gcgcattaga agaaggcatt
     3061 cgcaccgggg atttagcccg tggcgctgcc gccgttagta ccgatgaaat gggcgatatc
     3121 attgcccgct atgtggcaga aggggtgtaa tcatggctaa gacgttatac gaaaaattat
     3181 tcgacgctca cgtagtgtac gaagccgaaa acgaaacccc gctgttatat atcgaccgcc
     3241 acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc
     3301 cggtacgtca gccgggcaaa accttcgcca ccatggatca caacgtctct acccagacta
     3361 aagacattaa tgcctgcggt gaaatggcgc gcatccagat gcaggagctg atcaaaaact
     3421 gcaaagaatt tggcgtcgaa ctgtatgacc tgaatcaccc ctatcaaggg atcgtccacg
     3481 taatggggcc ggaacagggc gtgaccttgc cggggatgac cattgtctgc ggcgactcgc
     3541 ataccgccac ccacggcgcg tttggcgcac tggcctttgg tatcggcact tccgaagttg
     3601 aacacgtact ggcaacgcaa accctgaaac agggccgtgc gaagaccatg aaaattgaag
     3661 tccagggcaa agccgcgccg ggcattaccg caaaagatat cgtgttggca attatcggta
     3721 aaaccggtag cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg
     3781 atttaagcat ggaaggtcgg atgaccctgt gcaatatggc aatcgaaatg ggcgcgaaag
     3841 ccggtctggt tgcacctgac gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc
     3901 cgaaaggcaa agatttcgac gacgccgttg cctactggaa aaccctgcaa accgacgaag
     3961 gcgcaacttt cgataccgtt gtcactttgc aggcagaaga gatttcaccg caggtcacct
     4021 ggggaactaa cccaggccag gtgatttccg tgaacgacaa tattcccgat ccggcttcgt
     4081 ttgccgatcc ggttgaacgc gcgtcggcag aaaaagcgtt ggcctatatg gggctgaaac
     4141 cgggtattct gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc tgcaccaact
     4201 cacgtattga agatttacgc gcggcggcgg aaatcgccaa agggcggaaa gtcgcgccag
     4261 gcgtacaggc gctggtggtt cccggctctg gtccggtaaa agcgcaggcg gaagcggaag
     4321 gtctggataa aatctttatt gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt
     4381 gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg ttgtgcctcc accagcaacc
     4441 gtaactttga aggccgtcag gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg
     4501 ctgccgccgc ggctgtgacc ggccatttcg ccgacattcg caacattaaa taaggagcac
     4561 accatggcag agaaatttat caaacacaca ggcctggtgg ttccgctgga tgccgccaat
     4621 gtcgataccg atgcaatcat cccgaaacag tttttgcaga aagtgacccg tactggtttt
     4681 ggcgcgcatc tgtttaacga ctggcgtttt ctggatgaaa aaggccaaca gccaaacccg
     4741 gacttcgtgc tgaacttctc gcagtatcag ggcgcttcca ttttgctggc acgagaaaac
     4801 ttcggctgtg gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta cggttttaag
     4861 gtggtgattg cgccgagttt tgctgacatc ttctacggca atagctttaa caaccagctg
     4921 ctgccggtga aattaagcga cgcagaagtg gatgagctgt ttgcgctggt gaaagccaat
     4981 ccggggattc attttgatgt agacctggaa gcgcaagagg tgaaagcggg tgagaaaacc
     5041 tatcgcttta ccattgatgc cttccgccgc cactgcatga tgaacggtct ggacagcatt
     5101 gggcttacct tgcagcacga cgacgccatt gcggcttatg aagcgaagca acccgcgttt
     5161 atgcgttagt ttgtacaccg tccgatactc aatcgccaaa aagaaaaaag ccagcagata
     5221 atcactgctg gcgtggcgat taacaccaat actcagtcac acatgatgtc cgcaaatcag
     5281 tagtcagaat gactcccctc ccactgcatc agttcttcaa gcatggttaa tcgttgtggg
     5341 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
     5401 tgacgaatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
     5461 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
     5521 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
     5581 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
     5641 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgtg gtaaacgctc gcgtctgaca
     5701 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggac
     5761 cgtatcgacc aactagtgca gttagttggc gacaaagcga ctgtgaggca aatgctggtt
     5821 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
     5881 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tatcgcccgg
     5941 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
     6001 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
     6061 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
     6121 aatacgctgc cgctctattc gcatattgct gacattgtgt cgccgacgcc ctggacgctg
     6181 gatatccacc tcacgcagcc ggatcgctgg ttgccgttac tgctgggaca agttccggcg
     6241 atgatcctgc cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc catcggcacc
     6301 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
     6361 ttcttcggtt accgggcatt aatcgatgaa gttaacgtct gggttctgcc ggaaattgcc
     6421 gacgagccag ccggagggct gatgctaaaa ggtccacagg gcgaggaaaa agagattgaa
     6481 agccgcctgg aggaaggttg ctactattta ctgttcgata gccgcaccca tcgcggggcg
     6541 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
     6601 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcat
     6661 catgcccgca ccataacgag cgaaaaaccg gctggtctgg aaagcctcac cctgaccttt
     6721 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagc
     6781 caccaggtca cactggaaat caaagagatc agctacgatc agtggcatga aggagagatc
     6841 gagagcgata tctggcttaa cagcgccaac tttacgctgc cgctggattt ttcgctgttc
     6901 gcgcacctgt gcgaggtacc gctgctccaa cactgtattc caatcgactg gcaagtcgac
     6961 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccaaca actggtcgcc
     7021 agcaaagcaa tggtgccact tatccaccac tggctgatca ttcagggaca acgcagtatg
     7081 cgcggcctgc gcatgaacac cctcggctgg tttgatttta aatcagcgtg gtttgcgccg
     7141 ccggatccag agtagcttgc gccttttggc ggtcatgagc gcatttttta tattcagacg
     7201 gaatgataaa atacattact tttatatttt ctctgtttta tgcatcgtct gattagcgtc
     7261 gcaaataaga ttaaagaagg ggcattccag cccctctttc atattctgag ttaatcaata
     7321 acggcgcagt ctagccttgt tctggccgga aaggtcagct ttttcaggtt aacctcgcgc
     7381 aggtcagtca taccaacggc tattgttcgt ttcagcattg ctcaggtcaa cctcgagaca
     7441 gtcaatacca caaatgacaa ccccgttaaa gtaagcatta ctcagatcaa tccccccggt
     7501 cctggtgctc tttttcagct gcaactccag agtaaatatc gcctcatcgc caataaaaaa
     7561 actgacgcat aacgtacctt ctggcgagag tgaggccttc gcggccatat tttccggatc
     7621 ttttgcgtat tctttgagtt cattaaattt tatcagccag tgagttaact tatcacgcgc
     7681 cttcccgtcg acagccccgg taaagtattt tgagtagtca tcactattct ggaaataaaa
     7741 tgcatccttc agaatttgcc ttgccgcagt ctgggaatgt ttatctgact ggaagagatt
     7801 gcgtaacgtc tcccatagct cttcaaagat attcatattc tcggtctttt tgaccaagtt
     7861 agccatcctg ataatctgag aattagtaag gcctggatct ttagtggtat tactagcagg
     7921 gatactaatc tgttctgggt ttcttacttt catgctgata gctccactct gaataaaaaa
     7981 ctcagaataa gcgtcctgaa taagaatact gtcaaaaatc gctcattcac gcaataaacg
     8041 gttacgttac ctattttgac tgtagcgaag ggattctttc ggatcaacgc tctccagcta
     8101 tgcaaatacg ctcttgatag ctgaacgaat attggctttt ttaatacgcc ttctctatgg
     8161 attgctggtt gataacaaaa tcactacact aacgccgttc tcaacggggt gccacgcgga
     8221 cgcgtgcgct gagaaaatac ccgtcgaacc tgatccggat aacgccggcg aagggatttg
     8281 aggctccttc tcaagtcctt tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc
     8341 ttcccctgct gttgctgtgc acagcgcccg ttttcgctaa acccgttctg actgtttata
     8401 cctacgattc cttcgccgcc gactgggggc ctggtccggt ggttaaaaaa gcctttgaag
     8461 ccgactgtaa ttgcgaactg aaactggtgg cgctggaaga tggcgtttcg cttctcaacc
     8521 gtctacggat ggaaggcaaa aacagtaaag ccgatgtggt gctggggctg gataacaatc
     8581 tgttagacgc cgccagtaaa accggactgt ttgccaaaag cggtgtggca gcggatgccg
     8641 ttaacgttcc cggcggctgg aataatgaca ttttcgtacc gtttgattat ggctacttcg
     8701 ccttcgttta tgacaagaac aaactgaaaa acccgccaca aagcctgaaa gaactggttg
     8761 agagcgatca aaactggcgg gtgatttatc aggatccgcg caccagtaca ccggggctgg
     8821 gtctgttgct atggatgcaa aaagtctatg gcgatgacgc cccacaagcc tggcagaaac
     8881 tggcgaagaa aacggtcacg gtcaccaaag gctggagcga agcctacggc ctgtttttaa
     8941 aaggtgaaag cgatctggta ctgagttaca ccacctctcc ggcttatcac attctcgaag
     9001 agaagaaaga taactacgcc gccgcgaact tcagcgaagg tcactatctg caagtggaag
     9061 tcgccgctcg caccgctgcc agcaagcagc cggagctggc gcaaaaattc ctccagttta
     9121 tggtttctcc ggctttccag aatgcgatcc caaccggcaa ctggatgtat ccggtggcaa
     9181 acgtcacgct gcctgccggt tttgaaaaat tgaccaaacc cgcaaccacg ctggagttca
     9241 cgccagccga agtggcggca caacgtcagg catggattag cgaatggcaa cgcgccgtca
     9301 gccgttaatt cccggctggt taattccagg tgtaagcgcc gccacgctgg tggtagcggt
     9361 tgcgctggcg gcgtttctcg ccctgtggtg gaacgcgccg cagggtgact ggtcggcagt
     9421 ctggcaggac agctacctgt ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc
     9481 ggcgctactc tctgtcgtac ccgcgatatt cctcgcccgc gcgctctatc gcaggcgctt
     9541 tccgggtcgg ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctggt
     9601 tgctgttttc ggcattctta gcgtctatgg tcgccagggc tggctggcat cgctctgcca
     9661 atcgctcggt ctggagtgga ccttttcgcc ctacggcctg caaggcattt tgctggccca
     9721 tgtgtttttt aatctgccga tggcgagccg cttattactc caggcactgg aaaacattcc
     9781 cggcgaacaa cgtcagcttg ccgcccagct tgggatgcgc ggctggcatt ttttccgctt
     9841 cgtcgaatgg ccgtggttac ggcgacaaat cccgccggtt accgcgctta tctttatgct
     9901 ctgtttcgcc agcttcgcca ccgtgttatc gctgggcggc ggtccgcagg caaccactat
     9961 cgagctggca atctatcagg cgctgagtta cgactacgat cctgcccgcg cggcgatgct
    10021 ggcgctgatc cagatggtgt gttgccttgg gctggtgctg ttgagtcagc gactgagtaa
    10081 ggccattgcg cccggcacca cgctgctgca aggctggcgc gacccggacg atcgtctgca
    10141 tagccgcatt tgcgacacga tgttaattgt gctggcgctg ctgctgttgc tgccaccgtt
    10201 gctggcggtg atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg cacaaccggt
    10261 gctgtggcag gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt
    10321 agtgctgacc atgatgctgc tatggagcag tcgcgaactg cgggcgcggc aaaaaatgct
    10381 ggcgggccag gcgctggaga tgagcggcat gttgatcctc gctatgccgg ggattgtgct
    10441 ggcaacgggc ttctttttac tgctcaacaa cactatcggc ctgccacaat ctgctgacgg
    10501 cattgtgatt ttcaccaatg cgttaatggc gatcccctac gccctgaaag tgctggaaaa
    10561 cccgatgcgc gatatcaccg cccgctacag catgttatgt cagtcgctgg ggattgaagg
    10621 ctggtcacgc ttaaaagtag tggagctgcg cgccctgaaa cgcccgctgg cgcaggcgct
    10681 ggcctttgcc tgcgtgctgt cgattggtga ttttggcgtg gtggcgttgt tcggtaacga
    10741 tgatttccgc accctgccgt tttatctcta ccagcaaatt ggctcctatc gcagccagga
    10801 cggcgcggtc accgcgttaa ttctgctgct gctctgtttt ctgctgttta gcgtgattga
    10861 aaaaataccg gggcgaaatg ttaaaactga ctgatatcac ctggctttat caccatttgc
    10921 cgatgcgttt tagcttaacg gtagaacgcg gcgagcaggt ggcgatcctc gggccaagcg
    10981 gcgcgggtaa aagtaccctg ctgaacttga tcgccggttt tctgacacca gccagcggtg
    11041 cgctgactat cgacggcgtt gatcacacca cgacgccgcc gtcacgccgt ccggtgtcga
    11101 tgctgtttca ggagaacaac ctgttcagcc acctgacggt cgcgcagaac atcgggctgg
    11161 ggcttaatcc aggattgaag ctgaacgcgg tacagcagga gaaaatgcac gcgattgccc
    11221 gtcagatggg gattgataat ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc
    11281 gacagcgcgt ggcgttagcg cgttgtctgg tacgcgaaca gccgatttta ttgctcgatg
    11341 aaccgttctc tgcgctcgat ccggcgctac gtcaggagat gttgacgctg gtgagcacta
    11401 gctgccagca gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa gatgcggcgc
    11461 ggattgccac gcgctcggta gtagtcgccg acgggcgaat cgcctggcag ggtatgacca
    11521 atgagttgtt gagcggtaag gcgagtgctt cggcgctgtt gggaattaca ggttaatatg
    11581 cactgtcgga tgcggcgtga acgccttatc cgacctacgg gtgcgttttg tccgcatcaa
    11641 gcgccccaca caagactatg ccctaaactc caaccacttt acgcaaaata tcgatataca
    11701 ccggcattaa cggatggcga actaacacca ccagcgccac cacgccgatg gcagaaatca
    11761 acggcgtcag ccacaacaaa cgaccgcggg acaaataatg actcaaacga tcggttgctt
    11821 tgccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat accgccgtcg
    11881 ccagcagcaa ccatttaaac tcaccgctct gcattccggc tggaatatcg atcgccgcac
    11941 ccgccagaat ccccggcagg aagtaaaacg gtggccacag caggcagccg ataatattcg
    12001 gcgtgataaa ttttgccacc gggagatcca gcattcccgc caccattggc accagcggac
    12061 gcgtcgggcc aacaaaacga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat
    12121 gttcagtttt atcgagtaat gctttgttct tcttcagaaa tgaccaacga tgcaatggct
    12181 ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcagacaa ccgacgatcc
    12241 ctgccagcca ggcgtgccag aaacttaact cgccgctgcc aatcagcgct cccagccccg
    12301 ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca
    12361 ccaacaccac cgccatcaat gaatacacgg tggattgggt aataaagtgt tccagcaatg
    12421 cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
    12481 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
    12541 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
    12601 actcgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
    12661 atccgggtag tgctcaaaag cagcttcgcc tggctgatgc gttggtcctc gcgccagctt
    12721 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaggcaa
    12781 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
    12841 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
    12901 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
    12961 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
    13021 tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc
    13081 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
    13141 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcagaca
    13201 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
    13261 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccattggc ctcaatcggc
    13321 gttaaacccg ccaccagatg ggcgttaaac gagtatcccg gcagcagggg atcattttgc
    13381 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
    13441 atcagatatt gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc
    13501 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
    13561 aaaagtgtct ataatcacgg cagaaaagtc cacattgata atttgcacgg cgtcacactt
    13621 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
    13681 caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc
    13741 aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact gcaccaccgg
    13801 tgaagaaatc gccaccagcg tagagtggta tccccgttgg caaaaagggc aattttgtga
    13861 tgccccgaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc
    13921 actgaaaacc gtgcttgcag aacttagcgt cgaacagcgc gcagctgtgg tcgggattgg
    13981 cgttgacagt accggctcga cgcccgcacc gattgatgcc gacggtaacg tgctggcgct
    14041 gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacactgc
    14101 ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg ccgggcaatg ttgactactc
    14161 ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac
    14221 tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt
    14281 gccagctctg ctttccggta ccacgcgccc gcaggatatt cgtcgcggac gttgcagcgc
    14341 cgggcataag tcgctatggc acgaaagctg gggcggcctg ccgccagcca gtttctttga
    14401 tgagctggat ccgatcctca atcgccattt gccttccccg ctgttcactg atacctggac
    14461 tgccgatatt ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga
    14521 aagcgtggtg atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gtgcaggcgc
    14581 acagcctaac gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga
    14641 caaacagagc gttggcgagc gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt
    14701 ggtgcctgga tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg
    14761 gttcggtcgc gtactcggct ggccgctgga acagcttgcc gcccagcatc cggaactaaa
    14821 agagcaaatc gacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa
    14881 aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggcc gccgcacacc
    14941 gaacgctaac caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacgctcc
    15001 gctgctgttc ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg
    15061 ctttaccgat caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa
    15121 aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc
    15181 ctctgaccaa tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca
    15241 cgcagacatc ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc
    15301 gcgcagcgaa caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat
    15361 gagcgccgaa caacactatc ttccaacttc cgccccggca caggctgccc aggccgttcc
    15421 gactctataa ggacacgata atgacgattt ttgataatta tgaagtgtgg tttgtaattg
    15481 gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg
    15541 ttgtgaaagc gctgaatacg gaagcgaaac tgccctgcaa actggtactg aaaccgctgg
    15601 gcaccacgcc ggatgaaatc accgctattt gccgtgatgc taattacgac gatcgttgcg
    15661 ctggtctggt ggtgtggcta cacaccttct ccccggccaa aatgtggatc aacggcctga
    15721 ccatgctcaa caaaccgttg ctgcaattcc acacccagtt caacgcggcg ctgccgtggg
    15781 acagcatcga tatggacttt atgaacctga accagaccgc gcacggcggt cgcgagttcg
    15841 gcttcattgg cgcgcgtatg cgtcagcaac atgccgtggt taccggtcac tggcaggata
    15901 aacaagccca tgagcgtatc ggctcctgga tgcgtcaggc ggtatctaaa caggataccc
    15961 gtcatctgaa agtctgccga tttggcgata acatgcgtga agtggcggtc accgatggcg
    16021 ataaagttgc cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc
    16081 tggtgcaggt ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg
    16141 aaagctgcta caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc
    16201 tggaagcggc gcgtattgag ctggggatga aacgtttcct ggaacaaggt ggcttccacg
    16261 cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttcctggt ctggccgtac
    16321 agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa actgccgccc
    16381 tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg
    16441 actacaccta tcacttcgag aaaggcaatg acctggtgct cggctcccat atgctggaag
    16501 tctgcccgtc gatcgccgca gaagagaaac cgatcctcga cgttcagcat ctcggtattg
    16561 gtggtaagga cgatcctgcc cgcctgatct tcaataccca aaccggtcca gcgattgtcg
    16621 ccagcttgat tgatctcggc gatcgttacc gtctactggt taactgcatc gacacggtga
    16681 aaacaccgca ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg
    16741 atctgccaac tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca
    16801 gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca
    16861 cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcgctgcgc tggaacgaag
    16921 tgtattacgg gtttcgccgc taagtagccg catccggtat gtaacgcctg atgcgacgct
    16981 ggcgcgtctt atcaggccta cacgctgcaa ttttgtaggc cggataagca acgcgcatcc
    17041 ggcattcagc gcctgatgcg acgctgacgc gtcttatctg gcctacacgc tgcgattttg
    17101 taggccggat aagcaacgcg catccggcac gaaggagtca acatgttaga agatctcaaa
    17161 cgtcaggtat tagaagccaa cctggcgctg ccaaaacaca acctggtcac gctcacatgg
    17221 ggcaacgtca gcgccgttga tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc
    17281 gattacagca tcatgaccgc tgacgatatg gtcgtggtca gcatcgaaac cggtgaagtg
    17341 gttgaaggtg cgaaaaagcc ctcctccgat acgccaactc accgactact ctatcaggca
    17401 ttcccgtcca ttggcggcat tgtgcacacg cactcgcgcc acgccaccat ctgggcgcag
    17461 gcgggtcagt cgattccagc aaccggcacc acccacgccg actatttcta cggcaccatt
    17521 ccctgcaccc gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt
    17581 aacgtcatcg tagaaacctt cgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt
    17641 ctggtccatt ctcacggccc atttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac
    17701 gccatcgtgc tggaagaggt cgcttatatg gggatattct gccgtcagtt agcgccgcag
    17761 ttaccggata tgcagcaaac gctgctggat aaacactatc tgcgtaagca tggcgcgaag
    17821 gcatattacg ggcagtaatg actgtataaa accacagcca atcaaatgaa accaggctat
    17881 aatcaagcct ggttttttga tggattttca gcgtggcgca ggcaggtttt atcttaaccc
    17941 gacactggcg ggataccccg caaggaacag aggtctcctt ctggctggcg acggacaacg
    18001 ggccgttgca ggttacgctt gcaccgcaag agtccgtggc gtttattccc gccgatcagg
    18061 ttccccgcgc tcagcatatt ttgcggggtg aacaaggctt tcgcctgacg ccgctggcgt
    18121 taaaggattt tcaccgccag ccggtgtatg gcctttactg tcgcgcccat cgccagttga
    18181 tgaattacga aaagcgcctg cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc
    18241 cgccagaacg ctatctgatg gagcggttta tcacctcacc ggtgtgggtc gagggtgata
    18301 tgcacaatgg cgctatcgtt aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc
    18361 tcaagtgggt ttctattgat attgaaacca cccgccacgg agagctgtac tgcatcggcc
    18421 tggagggctg cgggcagcgc atcgtttata tgctgggacc agagaatggc gacgcctccg
    18481 cgctcgattt tgaactggag tacgtcgcca gccgcccgca gctactggaa aaactcaacg
    18541 cctggtttgc aaactacgat cctgatgtga tcatcggttg gaacgtggtg cagtttgatc
    18601 tgcgaatgct gcaaaaacat gccgagcgtt atcgtattcc gctgcggctg gggcgcgata
    18661 acagtgagct ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt gcccaggcta
    18721 aaggtcggct aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt
    18781 cattctcgct ggaaactgtc gctcaggagc tattaggcga aggaaaatct atcgataacc
    18841 cgtgggatcg aatggacgaa attgaccgcc gtttcgccga agataaacct gcgctggcaa
    18901 cttataacct gaaagattgc gagctggtga cgcagatctt ccataaaact gaaatcatgc
    18961 cgtttttgct cgaacgagca acggtgaacg gcctgccggt ggatcgacac ggcgggtcgg
    19021 tggcagcgtt tggtcatctc tattttcccc gtatgcatcg cgctggttat gtcgcgccta
    19081 atctcggcga agtgccgccg cacgccagcc ctggcggcta cgtgatggat tcgcgacctg
    19141 ggctttatga ttcggtgctg gtgctggact acaaaagcct gtacccgtcg atcatccgca
    19201 cctttctgat tgatcccgtc gggctggtgg aaggcatggc gcagcctgat ccagagcaca
    19261 gcaccgaagg ctttctcgat gcctggttct cgcgagaaaa acattgcctg ccggagattg
    19321 tgactaacat ctggcacgga cgcgatgaag ccaaacgcca gggtaacaaa ccgctgtcgc
    19381 aggcgctgaa aatcatcatg aatgcctttt atggcgtgct cggcaccacc gcctgccgct
    19441 tcttcgatcc gcggctggta tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa
    19501 ccaaagcgtt gattgaagcg cagggctacg acgtgatcta cggcgatacc gactcaacgt
    19561 ttgtctggct gaaaggcgca cattcggaag aagaagcgac gaaaatcggt cgtgcactgg
    19621 tgcagcacgt taacgtctgg tgggcagaaa cgctgcaaaa acaacagctg accagcgcac
    19681 tggaactgga gtatgaaacc catttctgcc gttttctgat gccaaccatt cgcggagccg
    19741 ataccggcag taaaaagcgt tatgccgggc tgattcagga gggcgacaaa cagcggatgg
    19801 tgtttaaagg gctggaaacc gtgcgtacgg actggacgcc gctggcccag cagtttcagc
    19861 aggagcttta cctgcgtatc ttccgcaacg agccatatca ggagtacttc cgcgaaacca
    19921 tcgacaaact gatggcgggt gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc
    19981 gtccgctgag cgaatatcag cgtaatgttc cgcctcatgt acgcgccgct cgtcttgccg
    20041 atgaagaaaa ccaaaagcgc ggtcgcccct tgcaatatca gaaccgcggc accattaagt
    20101 acgtatggac caccaacggc ccggagccgc tggactacca acgttcacca ctggattacg
    20161 aacactatct gacccgccag ctacaacccg tggcggaggg aatactccct tttattgagg
    20221 ataattttgc tacacttatg accgggcaac ttgggctatt ttgagcaaaa aaagagttcg
    20281 ccagatacca ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc
    20341 attcctggac ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg
    20401 caattaaaga tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc
    20461 gatacagaaa gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact
    20521 ttacttttcc catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc
    20581 cgcgtgatgt tcaaccctgg tgataccatt accagccatg acggctggca gatgcaagtc
    20641 gaagaagtaa aagaagaaaa tggcttgctg acctatatcg gtactcgcct ggatactgaa
    20701 gagtccggcg tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg
    20761 caggaccgcc tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc
    20821 gcgcgtaagt attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt
    20881 accagcctga tcccgcacca gctcaacatc gctcatgatg ttggccgccg ccacgcgccg
    20941 cgcgtcctgc tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctt
    21001 catcagcaac tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta
    21061 cagcatcagt ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc gctgtttgat
    21121 gatgagcgtt atgccgaagc tcagcacgat gcttacaacc cgttcgacac cgaacagctg
    21181 gtgatttgct cgctggattt tgcccgccgt agcaaacagc gtctggaaca tctctgtgaa
    21241 gccgagtggg acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagacgcg
    21301 ccgagccgcg aatatcaggc tattgaacaa ctggcagagc acgtgccggg cgttctgctg
    21361 ctgaccgcaa ccccggaaca gctgggaatg gaaagccact tcgcccgtct gcgtctgctg
    21421 gacccgaacc gtttccacga tttcgcgcaa ttcgttgaag agcagaaaaa ttatcgtcca
    21481 gtagcggacg ccgttgccat gctgctggca ggtaacaaac tgagcaatga cgaactgaac
    21541 atgcttggcg agatgatcgg cgagcaggat atcgagccgc tgttgcaagc agcaaacagc
    21601 gacagcgaag atgcccagag cgcccgtcag gagctggttt cgatgctgat ggatcgccac
    21661 ggcaccagcc gcgtgctgtt ccgtaacacc cgtaacggtg tgaaaggctt cccgaaacgc
    21721 gagctgcaca ccattaagct gccgctaccg acgcagtatc agacggctat taaagtctcc
    21781 ggcattatgg gcgcacgtaa aagtgcggaa gaccgcgccc gcgatatgct ctacccggag
    21841 cgtatttatc aggaatttga aggtgataac gccacctggt ggaacttcga tccgcgcgtt
    21901 gagtggctga tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgct
    21961 aaagctgcca ctgcgctgca actggagcaa gtactgcgcg aacgtgaagg tattcgcgct
    22021 gcggtgttcc acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc
    22081 gaagaagaca ccggcgcaca ggtactgctg tgctcagaaa tcggttctga aggacgtaac
    22141 ttccagttcg ccagccacat ggtgatgttt gacctgccat tcaacccgga tctactggag
    22201 cagcgtattg gtcgtctgga tcgtatcggc caagcgcacg atattcagat ccatgtgcct
    22261 tatctggaga aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca
    22321 tttgagcaca cctgcccgac cggacgcact atttacgata gcgtatacaa cgatctgatt
    22381 aactatctgg cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc
    22441 gagcaacatg aagcgctgaa agcacagctg gaacagggtc gtgaccgcct gctggaaatc
    22501 cactccaacg gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac
    22561 gataccaacc tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac
    22621 gatcgcggcg acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc
    22681 cctggcctgt cggaagatgg catcaccatc acctttgatc gtgaagtggc gctggcgcgt
    22741 gaagatgcgc agtttattac ctgggaacat ccgctgatcc gcaacggtct ggatctgatt
    22801 ctttctggcg ataccggtag cagcacgatt tcactgttaa aaaacaaagc gttgccggta
    22861 ggtacgctgt tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag
    22921 ctcaaccgct tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac
    22981 ctggcggcgc aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac
    23041 accggcagca aactggttaa cgccgtgcag caggatgttc acgctatcct tcaactgggt
    23101 gaagcgcaga tcgagaaatc tgcccgtgca ttgattgatg cagcacgtaa cgaagccgac
    23161 gaaaaactgt ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt
    23221 cgtgacgacg aactgaccgc cattgagagc aaccgtcagc aggtaatgga aagcctggat
    23281 caggcaggtt ggcgtctgga tgccctgcgt ttgatcgttg taacgcatca gtaacggagc
    23341 cgaaaatggg gatggaaaac tacaatccac cgcaggaacc ctggttggtt atcctgtatc
    23401 aggatgacca tattatggtg gtcaacaagc cgagcggttt gttgtctgtg ccgggtcgtc
    23461 tggaagagca caaagacagc gtgatgacgc gcattcagcg agattatccg caggcagaat
    23521 cggtgcatcg tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg
    23581 cggagcggga gttaaaacgc cagttccgcg agcgcgtgcc gaaaaagcag tatgtgaccc
    23641 gcgtctgggg gcatccatcc cccgcagaag gtctggtgga tctgccgctg atttgcgact
    23701 ggccaaaccg cccgaaacag aaagtctgtt acgaaacggg taaacctgcg cagacggaat
    23761 atgaagtggt ggagtatgcg gcggataaca cggcaagagt ggtgttaaaa ccgattaccg
    23821 ggcgttcgca tcaattgcgt gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc
    23881 gtttttatgc atcaccagaa gcgagagcga tggcaccgcg tttgttgcta catgcagaga
    23941 tgttgacgat tacccatccg gcgtatggca atagtatgac gtttaaagcg ccagcggatt
    24001 tttaaccttt cctgtacgtt tataccctca tcctaagggc tgcctcttgt acataaatcc
    24061 ccgagtccgg ggattttttg tttctgccaa actgctttca gttgttgcgt gtggctacgc
    24121 tttccattcg gtaattccct tcctgctcca cagcgaattt ttgaactttt tcttcacccg
    24181 ttgaccgtct cttatatttc attttatcgg gtctgttatg gtcctttctg attgttattc
    24241 atgggctaat tagcagtctg gttggcgatg cccatctcgg tgatccgcgt cgtacacgac
    24301 gccttgcgtc actagcctct tctctagctc atcatgcagg tctttctaat tgtgaaatca
    24361 tcccacgcta ctgctaaggt tgaaggggcg ttccagcccc ttcaacctta gcttattctg
    24421 gctcattcaa taattgcgtt gtcagtaatt tgttcagcag tactttgttt agtagcaact
    24481 tcttcagtat caattttttt gggggtgatt ttattaataa taattacctc atcatatagg
    24541 tgggtagcac cgtccagggt agcaccgtcc agggtagcac cgtccaggtt agcattccac
    24601 ccaggttcca agtaaaggac tttattgtga cctttcattg ttttgagacc aaggtaacaa
    24661 tccctcatgt caacgccggg aaggtaagcc tctctaagat caacgcccaa gttacctggg
    24721 cctttatgca gctgcaacgt ctgagtacat atctccttat tttcaataaa aaaagtgaca
    24781 cttaactcga tatcaccgaa ggtctgtgcg acccagctca tattgccctt tgcgaccatc
    24841 tcatccggat tttttgcgca tgttttgagt tcattgaata attccagact atggataaac
    24901 ttttctttct cattctcgtt gacagcccca gtaaagtatt ttgaggcgtt gatgttgcca
    24961 gcctttttat tcacgaaatt gaacagaagt ttataagcct cagtctgttt atctgaccag
    25021 aacagatgac gtaacgtctc ccacaagcgt tgaaagatat tcatcttcat gtccatcttg
    25081 tccccgctca ccatcttata aatatcgtta ttgctcatat ttaaaagtgt agccggcatg
    25141 cctggaactg atactttcat ggtaatagcc tcgcattgag ttaaaaactc atgatgcgcg
    25201 ctctgaataa atatactttc aaaaatcgct cattcagata tgtgtttttt atttttaaga
    25261 cgtaaaaata taattccgct cgccgtaaag ctctcaacct taagcagggt caacgcgata
    25321 aaaccaacag aaaccctccc gtaaaccgta tattgagcct gtccaccatt ctgagtaaac
    25381 gccttttctc aaagaagtga ccgccagggc ggccaccttt tatcgtgggc agttactcag
    25441 catcatttac agggtcattt aaaccctttc tgctgcttaa tcagctcata ggcctgctga
    25501 atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
    25561 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
    25621 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc
    25681 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
    25741 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
    25801 atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
    25861 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
    25921 tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcattttgc
    25981 gccgcagtac gggaagcgcc atgaagattc attcggtcca tcaactggct ggcgatatga
    26041 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
    26101 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
    26161 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
    26221 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
    26281 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
    26341 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
    26401 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
    26461 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
    26521 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
    26581 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
    26641 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctaaccacgc
    26701 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
    26761 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga
    26821 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
    26881 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggaaggtga
    26941 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
    27001 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
    27061 ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
    27121 gaacgcccgc tttaaggtgg gtccggtatc gatcttttat agcccctatt tgcagttgcc
    27181 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
    27241 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
    27301 cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat tccgctacct
    27361 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
    27421 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
    27481 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
    27541 cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
    27601 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
    27661 tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg ttaattacta
    27721 ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc actttgttaa
    27781 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
    27841 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
    27901 gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg aatccgttaa
    27961 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
    28021 gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt acgtgccgta
    28081 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
    28141 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
    28201 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
    28261 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
    28321 tgacgacaaa acgggttcac tggtgtgggc aggcgatact tactggcgta tctccgagcg
    28381 ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag cgaccagtaa
    28441 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
    28501 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
    28561 gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc gttggtccat
    28621 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
    28681 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
    28741 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
    28801 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
    28861 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
    28921 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
    28981 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
    29041 tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct gctcaggcaa
    29101 ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
    29161 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    29221 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    29281 gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    29341 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    29401 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    29461 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa
    29521 gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    29581 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    29641 aggagttgcc cgggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    29701 gcccgattcg ttccggcgtt ggcttccata tactgaaagt taacgacctg cgcggcgaaa
    29761 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    29821 tcatgactga cgaacaggct cgtgtgaaac tggaacagat tgctgctgat atcaagagtg
    29881 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    29941 agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc cgtgacgccc
    30001 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    30061 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    30121 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    30181 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    30241 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    30301 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    30361 gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg cacaaccgca
    30421 aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    30481 gttagcgatt gaaaatgggc attacgtggt ggagacgctg gcgcgagcgt gtgatggctg
    30541 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    30601 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    30661 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    30721 gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga ttgctatttt
    30781 gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    30841 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    30901 tgtgctcgac gagctgcggg cgcaggggat gaaactcaac gggccgctgc ctgccgatac
    30961 cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt accacgatca
    31021 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    31081 gccctttatt cgcacatcag tggaccacgg caccgcactt gaactggcgg gacgtggcga
    31141 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    31201 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    31261 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca aaagggccag
    31321 gcaatggtcg aaatcggccc cggtctggcg gcattgaccg agccggtcgg cgaacgtctg
    31381 gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    31441 ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa ctttggcgaa
    31501 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    31561 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    31621 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc gtatggtcga
    31681 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt tccgccgtca
    31741 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    31801 atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga agcctttaac
    31861 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    31921 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    31981 atggcgaact atctggcgga gaacgcgcct tttcaggaga gttaacgatg atcaattcgc
    32041 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    32101 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    32161 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    32221 aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag tacaccagcg
    32281 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    32341 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    32401 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    32461 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg
    32521 atctggtcgc gcgcggcccg ggttcgctgg atgttctgct ctatgtgaaa tccttaggcg
    32581 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcagta tttgccggga
    32641 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    32701 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    32761 tgatggccca cgcagggatc acgccgcagt gggatctgca gaccgccaaa gagtgcgcgc
    32821 gcgatgtaga agcggtgctg tcgagtgact cctatccctt ctttctcgac gctatgtacg
    32881 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cggtttatca
    32941 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    33001 aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt cctggacctg
    33061 tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    33121 cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta acctgcctgc
    33181 gctgggaaga taaacagtat tttgtccagc cgtcgaaccg gcataaggat ttgggtgagg
    33241 gagaggcggt cgcgtcttaa atcccgtcga gcataaaatc taatgctcct ttaatcaacg
    33301 ttcggtgcgc gctggcatcg caaaatttcg ttcccagcgc gttaactgga acagttgcag
    33361 tctcatgcgt catgagcaca tattctttgc cgtctaccag taaaaggatt gggttaagcc
    33421 gttctggtgg gcgaatacgg ctaaatcgtt caataggtgt taatggaata acaatacggc
    33481 gattaatcac accaataatg tcgctggtaa catcgataac aaagggaaac gcggcgttcc
    33541 tgctgcgact gcggtatacc gtaaattgca tcgctagaag ctccggtact catcagagaa
    33601 acatccggtt tcatcagcca attgatttaa cgcagcgata gcttcagcgt tctcttcacg
    33661 ccaacgtgtt gctgcatgtt ttttaagttc agcattgagc gcaaccgtaa gggtggcgct
    33721 catatttaag cctgcatcac gagcgcgatt gactaactca cggtcgacgg tgacggtcac
    33781 actttgtgtg gtacgttttg cagtcatgat atcgtgctcc tgtacaattt atacgtctag
    33841 tatacacgga tatctgcacg tatcagtgat gccggaattc taatatacaa aattaccgcc
    33901 gctccagaat ctcaaaacaa tagctgtgag agttctgcgc atcagcgtcg tggaattcgc
    33961 tgaataccga ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt
    34021 ctgcgtcgat atgcgtcagg tacagttttt gcgctttcgg caggaactgc tcataaacgc
    34081 gaccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcatcca
    34141 ccgacttcac ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt
    34201 tgcgtcctgg caacggacga ccgatggatt ccctggtatg gcggcccata attacgggtt
    34261 tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt
    34321 tttccatgcc gataacgcga tctaccgcta acgccgcaat cagactgatc attgagattt
    34381 cccgataaaa aaaattgtcg ccactatacg taaagcgtaa accgtcgtcg actggtgcga
    34441 ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc cacgctctct ccctggactc
    34501 gccgcattac aatgaaacaa aaacaaacag ttagctgtaa agtgtgattt acgtcactct
    34561 ttattaggat gagggtttcg tttccggttc atccgccatg ttgccggtat gtttaccttc
    34621 ttctgttccc tgccagccat gtcgttgaat taatgacaga tgttcgcggt cctcggtgat
    34681 gatctcactt aacatcgcgc tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt
    34741 ctcaaccatt gccatctctt ccaccatctg aatattaaag cggcggaaca catcggcacg
    34801 ttctcgcgct tcatacggcc ccagacctaa actttccagc gccagacgcc cggttttcag
    34861 cgcaccttca aaggtttcac gctctggctt ttcaacgcct gcctgacgca aacaaatgta
    34921 gtgatcgaca tcgcgggcgc gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat
    34981 ctccgtcagt tgcaggttgg tttgcggatc gtcaatggcg ttaatcagca cttccgcttt
    35041 cgccgctccc gcagattcca gtaaatccat ccgcgtggca tcgccataaa acactttcat
    35101 accaaattta cgcagcgttt cgatatggtc cggatcgtga tcgagcacca ccattttcac
    35161 cccgctggag agcagtaaac gtccggtgat ctgcccgaaa cgaccgaatc cggcaatgat
    35221 tacccgcggc tgttcttcgt cgatctcatc ggcttcacgc gcctcttcag tagaagattg
    35281 ctcaaggcga ttgaggatca ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc
    35341 cagggtcagc gatttcgccc actccggctc cagcacattc gccatctgcg ccgcgccaaa
    35401 taccacaaag gcaaactcac tgccctgccc taacaacacc gcaaaccagc gacgctgttt
    35461 atttggcacc tgcaacggtc gggcaatcag ccacagcatg gcgattttga tgatgaggaa
    35521 accgagcagc aaaatgacaa tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga
    35581 catgccaaca ccgatgaaaa acagccccaa cagcaaacct ttaaagggtt cgatatcgct
    35641 ttccagcgca tgacggtatt ccgagcttgc cagcagtacg cccgccagaa acgcgcccat
    35701 cgccatcgac aagccgacct cttccagcag caaaccaaag ccaaacacaa ggaataacgc
    35761 cacggcacta aacacttccc gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt
    35821 gacatagcgc cccagcagta ccaccagcac cagcgcgccc gccactttta acgccgagag
    35881 agcaaatgcg cccatcgtcg tcgaggcact gctcgccgcc agtagcggga tcatcgccac
    35941 cagcgggatt gccgcgatat cctggaacag cagcaccgca aaggcactgc ggcccatttg
    36001 cgtcaccatc agattgcgtt cattcatcgc ctgcatggca atcgccgtag aggagagcgc
    36061 cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag
    36121 ccccagcagg ccgccgcaaa tcaccatctg caatgcgcca ccgccaaaca ctgccgcacg
    36181 cagcttccac agcctttgtg gatcgagttc gaggccgata ataaacagca tcagcaccac
    36241 gccaatctcg gcaaagtgca gaatagattc ggcatcggtc accagtcgca gcccccacgg
    36301 gccaataatg cagccggcga tcaggtagcc aagtaccgat cccagaccaa gacgtactgc
    36361 aatgggtaca atcagcgccg ccgaaccgag ataaatcagc gcctgaacca gcgtatggct
    36421 atccatgatg ggcctcctgc cattccagca gacgttgctt atagtgacgc gcctgccctt
    36481 cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc aaacggtggc agccagttca
    36541 gcccgcagta gattgccgtc gcctgtagcg gctgcgacag cacatcaaag cccggatgcg
    36601 caccaatttc aaaatggctt tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc
    36661 catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg cgagaagact ttatcgatcc
    36721 aaagtttaag gagcggagga atgctgtacc actgcatcgg gtgctgccag acgatcagat
    36781 cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat attgaagtca ggatagagtt
    36841 gataaagaga gcgaatttcg acgccttcca gcgtccttgc ctgttcaaac atccgtttat
    36901 tcgcatggga atgatgcgga tacggatgcg cataaattat aagaatcata ggtagcccgc
    36961 gtgtttacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa
    37021 taatattgat tcagaaaaga gataaatctg aacaaattac cccattctct gcccgcggat
    37081 cgtcatattg ctggtctgcg ccagtgacat ccctcgagtc tccggggcaa acgctacgga
    37141 aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac tgataccgta
    37201 attattgata aagatcggta gtgcccaggt cgaaacaatg gtgccaatac gacttaagga
    37261 cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat taggatagag
    37321 ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg ccatcactac
    37381 cagccagatc cccatatccg ggatcagccc caaaaccgcc agcgccagcg tcatcatggc
    37441 aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca tcggcggaat
    37501 acagccgagc ataaagaaca ggctaatcac cacgttcccc agtgccgcgt ttttgccaac
    37561 acccaatccc aacaaaccaa cgatttgcgg gccaaaggtg taaatggcga acattgggat
    37621 cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa
    37681 cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa
    37741 cagtttgatc atcatctctt cgcactcttt tactcgccct ttgcgtaata accagcgggg
    37801 agattcaggc agttcgaatc gaccaatcaa aatcaacaaa caggggatcg ccgcgctacc
    37861 cagcatccag cgccagccgc cttccacatc ataaagccag tagccgacca gatcggcaca
    37921 ggtcgcgccg acataccaca tggcggcgat aaagctgatg gaaaacgccc gctgacgggt
    37981 actggagaac tcagtgatca tcgaagtggc gatgggatag tctgcaccga tgacaatgcc
    38041 gataagtacc cgcatcacca acagttcgac gggggatgaa acaaacatcg tcgccaccga
    38101 tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga ctttatcgga
    38161 aatataacca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa
    38221 gccaatccag tcagcgtcca gttttagcgc cggcgtcagt tgctccagcg ccacgccaat
    38281 cattaccagt atataaccat ccagaaacgg accaccgctt ccccacagca aaatgcggcg
    38341 gtgaatagag gaaaatttaa gatcgtcaaa gtttctggac ggttgcatgg gttattcctg
    38401 ttttttgcaa tgagagtcag tgcggcgtta cgtatcaaac caacatcagc cgtaacggaa
    38461 ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc ccagccccag
    38521 aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc
    38581 ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagcg cctgtttatc
    38641 ggcatcggct ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt tattgacgcc
    38701 cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt catgccatcc
    38761 ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt gcgcatcagt
    38821 tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcctaccgc cagttccggg
    38881 tagccgctaa acatgcgtgg gttatcaagg aaggccggta gtttctggta catacgcata
    38941 tcgcgcagcg ggccactctc aagatgctga cgatattccg cgagtttttg cttactgaaa
    39001 tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc ggcaatcgcc
    39061 agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc atcaccggca
    39121 atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc cggcactacg
    39181 tgagcggaat attccaccag cttgccaccc gcgatcagcg gtgcaacggc cggatgttgt
    39241 ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg atgcagacca
    39301 caaaccagcc ccagcgacag ggtgttttca ttggtataaa gaaaaccgcc gcccatcagg
    39361 ccatcggtgg gtgatcccgc aaataggcaa gccgccccct gattaccctg caactgaaaa
    39421 cggtcttcaa taaccgactt cggtaactcg atcagttcct tcacgccaac cgccacatcc
    39481 gtcggtttaa cgcgttttgc catccccagc ttttcggcaa ggatggagtt cactccatca
    39541 gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg cttctacacc gacgactttg
    39601 ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct
    39661 tcggcctgct ccatcagcca ggcatcaaat ttactgcgca aaacggagta agaacgctgg
    39721 gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt ttccgtcata
    39781 aacgcgagtt tttcatgggt gatcaggcgt tctaccgggg cggattcggc gaaaccagga
    39841 ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg
    39901 gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac cagtgcggca
    39961 accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc ggacataaga
    40021 gctccctgtc agcggcagga ccaaaatgca gccctgccag agtggatcaa cgcgctaaag
    40081 ctgcggtcag cgccggaagg atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact
    40141 ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac gcgttagcgc
    40201 caaccatgtg ctggatctgc ccggagatcc ccacagccag gtacagttca ggtttcagca
    40261 tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt tctgccaccg
    40321 gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt
    40381 tctctttgct gccaataccg cgaccgacgc tgaccaccag gcgggctttg tcgagatcga
    40441 cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc
    40501 actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc
    40561 cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca atcgccagac
    40621 caccgtaaac catatgtttc actgttgctt taccgtcctg tacgctgacg gtgctggcat
    40681 cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat ttgccgcgac
    40741 gggtgtttgg caacagcacc aggccgtctg caccgtgctg gcgaatagtg tcagccatga
    40801 cactggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc cagacatgat
    40861 tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag gcattgattt
    40921 gattagctaa agcctgcgca ccgttcatca gttccggcag acgagaaggg gtatcgctga
    40981 atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga ctttgcgaag
    41041 attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg
    41101 ctcgcgctgt ttcggcgcgg caacctgttg ttctgaccat gctgcctctg cgttaaaacc
    41161 gatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa tggctttcat
    41221 cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag gcagtggaat
    41281 gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat ctgccgtcag
    41341 ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc ctaccagcaa
    41401 accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca aatcaaagcc
    41461 tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa
    41521 ctggtcatca atcaccacaa tcagttcatc cggaccgcgc gatagcacat ctttacgccc
    41581 tttggcgttg gtcagggctt taccgcccac acttagggcc gtcacctgcg cctctgctgc
    41641 ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc ttattttggc
    41701 atcggcttta ctgaagtcta acgaaccatc agcattattg accgcaatat cctgttcatc
    41761 aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca tgaaggaaac
    41821 gtcgttcata tcggtggtgt taattacaga tatcatgccc tttaatttgg aaataaaact
    41881 ttaatcacca atattgaaaa tgtcacacgc agataaatcc aactttcaat attgttaagt
    41941 tcctcaccaa tattgaaaac acggcgtagc aaaaagaaat tttcaatatt gttttatgga
    42001 tcaccaatat tgaaagtcag atgctatttt tttactcgca acataacata acaaactgat
    42061 taataatcaa aattaacgag aaaagcgccc tctattcacc cgaaacaaaa atgtgataac
    42121 aatcacagaa tgcagcttat tgaataccca ttatgagtta gccattaacg cgtccatgag
    42181 gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg caaaataaat
    42241 attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg ggaatagaac
    42301 cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt acagtcagag
    42361 atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc aatgtatggg
    42421 gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg ctggtgtttg
    42481 gcccgtatgc caaaaagcgt ttaggtaacg aaccacctga atttagtacc gccagttgga
    42541 tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga tcgattgaga
    42601 tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca ggagcgaaag
    42661 agttggggct ggcttacagc ttgttcctct ggggacctct gccgtgggcc acttacagct
    42721 tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg attcgcccca
    42781 gctccaccct ggtgccgctg gtaggtgaaa aacacgccaa agggttgttc ggcactatcg
    42841 tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg ggccttgcca
    42901 cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc ctgcaactgg
    42961 acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgcc tgcggtctgc
    43021 aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg atgctgggtt
    43081 gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat tcggtgggga
    43141 tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct aaaggcggct
    43201 tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc atccagatga
    43261 gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc ttcggcatgg
    43321 tgatgggact gacagcatcc acctggatcc tgtggactgt actcggtagt aacactctgc
    43381 tgttgataga taaaaacatc atcaacattc caaatctgat cgaacagtac ggtgtggcgc
    43441 gcgccatcat cgaaacctgg gccgctctgc cgctcagcac cgccaccatg tggggcttct
    43501 tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat accctggcga
    43561 tgtccacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg cgtatcggtt
    43621 ggtcaattct ggttggcatt atcggtattg ttttgctggc gctcggcggc ctgaaaccga
    43681 ttcaaactgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc
    43741 tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc aaaatatcaa
    43801 gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc gccggtatcc
    43861 gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac cgtgacagcg
    43921 tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt ctgctgatcc
    43981 ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg tggatggagc
    44041 tgggacgtct gggggcacca acctatgtac tgtaccagtt gccgggcggg ttcaatacct
    44101 tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc ggcaccggta
    44161 agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg ggtagcctga
    44221 aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag tgttttatta
    44281 ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct tctccggaca
    44341 aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac
    44401 ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac gatgtggaac
    44461 tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc aaagaagagt
    44521 tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg tgcgcctttg
    44581 aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc
    44641 agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg aaaaacatgc
    44701 tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat gcagcgatgt
    44761 gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg caggtgctgg
    44821 gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag
    44881 accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt gcggtgctga
    44941 agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt atcaggccta
    45001 cgggagcacg aatgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgctac
    45061 ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg ggccgttggc
    45121 cggattgcgc gttgtcttct ccggtatcga aattgccggg ccgttcgccg ggcaaatgtt
    45181 cgcagaatgg ggtgcggaag ttatctggat cgagaacgtc gcctgggccg acaccattcg
    45241 cgttcaaccg aactacccgc aactctcccg ccgcaatttg cacgcgctgt cgttaaatat
    45301 tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg atatcttcat
    45361 cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag tactgtggca
    45421 acacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg gcaccgagga
    45481 gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt acctgattca
    45541 gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt acttttctgg
    45601 cctgaccgcc accacggcgg cgctggcagc actgcataaa gcgcgtgaaa ccggtaaagg
    45661 cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt acttcatgat
    45721 ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag atccctacta
    45781 tgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac tggtgggcat
    45841 tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg gtacgccgga
    45901 aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc cactggtaga
    45961 agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag aacgctttgc
    46021 cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa gcaatccaca
    46081 gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca cctgcaaagg
    46141 gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg gaatgccctc
    46201 acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa acgacattca
    46261 ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg tcagtcggct
    46321 tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt gcaatggata
    46381 tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt tacggtcata
    46441 aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt tatcttgagt
    46501 taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt cgcaaaggcg
    46561 acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg ttcgggctgg
    46621 caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag gaaagtgcgt
    46681 ggatcctgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattc tatcctatgt
    46741 atcaacagat tcagcaagaa gatgccactc aattgcggca catttgcctg acagatgtgg
    46801 cacttcccgc tgatgatggc gtgagttcgt ttactcaact gaaaaatcaa caacctgcca
    46861 ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc ttcacctccg
    46921 gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc ttcgctggat
    46981 attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacctgacg gtaatgcctg
    47041 cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc ggggccacct
    47101 ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggtacag aagtaccgcg
    47161 ccaccattac cgaatgtatt ccgatgatga ttcgtacgtt gatggtgcag ccgccttcag
    47221 cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg tcggagcagg
    47281 aaaaagacac attttgtgaa cgcttcggtg ttcgcttgct gacgtcttat gggatgacgg
    47341 aaaccattgt gggcattatc ggcgatcgcc ctggcgataa acgacgctgg ccgtcgattg
    47401 gtcgggcggg gttttgctac gacgcggaga tccgcgacga tcacaatcgc ccgctcccgg
    47461 caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc ttcaaagagt
    47521 attttctcaa cccgaaagcc actgcaaaag tgctggaagc tgatggctgg ctgcataccg
    47581 gcgataccgg ataccgcgac gaagaaggct ttttttattt catcgatcgc cgctgcaata
    47641 tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc atcgccaccc
    47701 atccaaaaat tcaggatatc gtggttgtgg gtattaaaga ttcgattcgc gatgaagcca
    47761 tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa tttttccgct
    47821 tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc agaaaagatc
    47881 tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg aaacggcagg
    47941 gaactaccct gccagctaat aatcacgctc taaaacaata tgcatttttt gcagggatgt
    48001 tgtcgtccct gaaaaagcaa aaatggagaa aaggaatgag tgaatcatta catctgaccc
    48061 gcaatggatc aattctggaa attactcttg atcgcccaaa agcgaatgct attgatgcaa
    48121 aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg caattacgtg
    48181 tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat ttaaaagcgg
    48241 cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg ggattaaccg
    48301 aaattttcaa tctcgacaaa ccggttatcg cagctgtgaa cggctacgcc tttggcggcg
    48361 gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc agcttcgccc
    48421 tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt ctgccgaaga
    48481 tcctgccgcc tgccatcgtc aatgaaatgg tgatgaccgg cagacgaatg ggcgcagaag
    48541 aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg atggataacg
    48601 cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg gcgctgaaag
    48661 agatctaccg caccaccagc gaaatgccgg tagaagaatc gtatcgctat attcgcagcg
    48721 gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa gggccgctgg
    48781 cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga gctattacgc
    48841 ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca gtgccgtctt
    48901 gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct cactgcgtgg
    48961 tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct gcattatgca
    49021 tggctacacc gacactgaca ccatcgttgg ggaaaacggc catatcgggc acggagcgat
    49081 cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg tgattatgga
    49141 tggcgcggtc attggcgaag agagcattgt tgccgccatg agctttgtca aagcaggctt
    49201 tcacggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac gcagtgttag
    49261 tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc ttgtcgggcg
    49321 ctgtcatgca tcgttacatg aaacacagcc actgaggcaa atggaggaaa atcgcccccg
    49381 tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc ggcgtaaacg
    49441 ccttatccaa ctctgctgga gagcaaccgt aggccggata agatgcgcca gcatcgcatc
    49501 cggcgaccag gtcgcacggt atcatcgaaa tgacattaac gacgcatact ctttgacaac
    49561 atcatctgcc acttctgctc gcggttaagt tccgtgagcg gaacggcagg aatacgcggt
    49621 gtctttctta cgccgactaa tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc
    49681 tcatcgatat cgaccacttt aaccagtcgc tgacactggc atccccgccc ttccagctta
    49741 ttagggatca tcttaacttc gcagcttatt tctgtgactt ccgacagaat ataagtcagg
    49801 gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag agatctctct tgctatcacc
    49861 caccgatttt cagccatcat ccattcggcg attaacaagt agagtggttt ttcaacatat
    49921 ccttcacaca tacgaactcc agtttcacaa attaaaagtc tggctatgac tcgttttgaa
    49981 gcaattaaac aaggccatat taaaattgtg gatatatcga tagtatgcaa cttcaccgtc
    50041 gataagtgtg agctaaatcc tgcttatgtc atcaaaaata tcgattcccc aaaagattta
    50101 cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttagttattc gatcaaatta
    50161 gcggatgaaa aatatccgcc atgacacgct attatttgat ctgtgcgtgc atttcctgca
    50221 ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt
    50281 tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc acgcgggagt
    50341 cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg ccattcttga
    50401 tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc ggattgatac
    50461 ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca
    50521 gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg
    50581 cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca aacgcttcag
    50641 cgaaggtgcg gcccacgccc atgacttccc cggtagaacg catttctggc cctaacagcg
    50701 ggtcaacgcc cgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg
    50761 ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc acacgcgccg
    50821 ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta cgcgccgcac
    50881 gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc acgttcatca
    50941 ggccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatt acatcctgaa
    51001 tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag tgcacgcccg
    51061 cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg cagatggcat
    51121 ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc gcatcgttag
    51181 acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag acgatttcca
    51241 tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca atctcttttg
    51301 ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt ttcagtttca
    51361 gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata gcatccgggc
    51421 tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc agcggggtct
    51481 gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt tccagcacat
    51541 cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg
    51601 tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg
    51661 cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga ccgccgccaa
    51721 gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg
    51781 agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga
    51841 ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt acgcccgcca
    51901 gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt
    51961 tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt tcttcaatct
    52021 gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca gacaggcccg
    52081 cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc agttcgcgac
    52141 ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaat ccagtcgcac
    52201 cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca
    52261 tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg gcgaattttt
    52321 cgaagttgaa gcgaggaatt ttggtaacca cgtaatcgat ggacggctcg aaggaggccg
    52381 gagtacgtcc gccagtgatg tcgttcatca gttcatcgag ggtgtaaccc accgccagtt
    52441 tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg
    52501 acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc accgcaaact
    52561 ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc gaggcgttac
    52621 gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg atggagtcac
    52681 cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg cagttgtcgt
    52741 ttttatcgcg caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca
    52801 gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca aactcttcgc
    52861 ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg
    52921 ggaagcccac gtcagcggca accgccagcg cttcttccat cgtatgtgcg ataccggaac
    52981 gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt
    53041 tatcaatcgc atcggcagtg gcaccaatca tggtgacgcc gaactcttcc aacacgccct
    53101 gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg
    53161 cgtccgggcg ctctttttca ataatcttac gtaccacttc ccagtgaatc ggctcgatgt
    53221 aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa
    53281 tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag tagtcaaact
    53341 cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt tttatatctg
    53401 tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat
    53461 taactcgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg
    53521 gtgaccctgg aagctgaacg ccggtttatc ggtgcgatga atgccctgta acgtaccgtc
    53581 gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa
    53641 accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa ccggatggtt
    53701 gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca gcgccagcag
    53761 ctgatgaccg aggcagatgc cgaagaccgg aatatcggtt tcgaggaatt tctggatggc
    53821 ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc
    53881 tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca
    53941 gcctctatcc accagcatcc gcaggatgtt gcgtttggca ccaaaatcat aagccacgac
    54001 gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca acgtccagct
    54061 cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccatt
    54121 cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc
    54181 gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc
    54241 gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt cttcggtatt
    54301 acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg catgtacctg
    54361 agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat aagtaagagt
    54421 aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt
    54481 attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg
    54541 ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca gagaatattc
    54601 actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa atctgcttac
    54661 cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag tcaacttaat
    54721 ggcaggattt tttatattat tttgtggctc atgcatgttt tacaggcatt aagcgacaaa
    54781 aagatcatct aacctgttct ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa
    54841 acgagcaggc aaaagtggac caaatggtca aaatttacat taagac
//
LOCUS       DAZGXS010000015        54320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGXS010000015 DAZGXS010000000
VERSION     DAZGXS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..2303)
                     /gene="pbpC"
                     /locus_tag="ACHKG5_001054"
     CDS             complement(<1..2303)
                     /gene="pbpC"
                     /locus_tag="ACHKG5_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0867201.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFS"
     gene            complement(2304..7265)
                     /gene="yfhM"
                     /locus_tag="ACHKG5_001055"
     CDS             complement(2304..7265)
                     /gene="yfhM"
                     /locus_tag="ACHKG5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0867202.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7472..8317
                     /gene="sseA"
                     /locus_tag="ACHKG5_001056"
     CDS             7472..8317
                     /gene="sseA"
                     /locus_tag="ACHKG5_001056"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0867203.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8660..8785
                     /gene="timP"
                     /locus_tag="ACHKG5_001057"
     CDS             8660..8785
                     /gene="timP"
                     /locus_tag="ACHKG5_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0867204.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8817..9593)
                     /gene="sseB"
                     /locus_tag="ACHKG5_001058"
     CDS             complement(8817..9593)
                     /gene="sseB"
                     /locus_tag="ACHKG5_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0867205.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9736..11019)
                     /gene="pepB"
                     /locus_tag="ACHKG5_001059"
     CDS             complement(9736..11019)
                     /gene="pepB"
                     /locus_tag="ACHKG5_001059"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0867206.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11197..11397)
                     /gene="iscX"
                     /locus_tag="ACHKG5_001060"
     CDS             complement(11197..11397)
                     /gene="iscX"
                     /locus_tag="ACHKG5_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0867207.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11409..11744)
                     /gene="fdx"
                     /locus_tag="ACHKG5_001061"
     CDS             complement(11409..11744)
                     /gene="fdx"
                     /locus_tag="ACHKG5_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0867208.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11746..13596)
                     /gene="hscA"
                     /locus_tag="ACHKG5_001062"
     CDS             complement(11746..13596)
                     /gene="hscA"
                     /locus_tag="ACHKG5_001062"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0867209.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13613..14128)
                     /gene="hscB"
                     /locus_tag="ACHKG5_001063"
     CDS             complement(13613..14128)
                     /gene="hscB"
                     /locus_tag="ACHKG5_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0867210.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14224..14547)
                     /gene="iscA"
                     /locus_tag="ACHKG5_001064"
     CDS             complement(14224..14547)
                     /gene="iscA"
                     /locus_tag="ACHKG5_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0867211.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14564..14950)
                     /gene="iscU"
                     /locus_tag="ACHKG5_001065"
     CDS             complement(14564..14950)
                     /gene="iscU"
                     /locus_tag="ACHKG5_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0867212.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14978..16192)
                     /gene="iscS"
                     /locus_tag="ACHKG5_001066"
     CDS             complement(14978..16192)
                     /gene="iscS"
                     /locus_tag="ACHKG5_001066"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0867213.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16304..16792)
                     /gene="iscR"
                     /locus_tag="ACHKG5_001067"
     CDS             complement(16304..16792)
                     /gene="iscR"
                     /locus_tag="ACHKG5_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0867214.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17093..17830)
                     /gene="trmJ"
                     /locus_tag="ACHKG5_001068"
     CDS             complement(17093..17830)
                     /gene="trmJ"
                     /locus_tag="ACHKG5_001068"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0867215.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17949..18752
                     /gene="suhB"
                     /locus_tag="ACHKG5_001069"
     CDS             17949..18752
                     /gene="suhB"
                     /locus_tag="ACHKG5_001069"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0867216.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18897..19751
                     /locus_tag="ACHKG5_001070"
     CDS             18897..19751
                     /locus_tag="ACHKG5_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0867217.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19942..21222
                     /gene="csiE"
                     /locus_tag="ACHKG5_001071"
     CDS             19942..21222
                     /gene="csiE"
                     /locus_tag="ACHKG5_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0867218.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21214..22353)
                     /locus_tag="ACHKG5_001072"
     CDS             complement(21214..22353)
                     /locus_tag="ACHKG5_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0867219.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22513..23403)
                     /gene="hcaR"
                     /locus_tag="ACHKG5_001073"
     CDS             complement(22513..23403)
                     /gene="hcaR"
                     /locus_tag="ACHKG5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0867220.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23539..24900
                     /gene="hcaE"
                     /locus_tag="ACHKG5_001074"
     CDS             23539..24900
                     /gene="hcaE"
                     /locus_tag="ACHKG5_001074"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0867221.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24897..25415
                     /gene="hcaF"
                     /locus_tag="ACHKG5_001075"
     CDS             24897..25415
                     /gene="hcaF"
                     /locus_tag="ACHKG5_001075"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0867222.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25415..25735
                     /locus_tag="ACHKG5_001076"
     CDS             25415..25735
                     /locus_tag="ACHKG5_001076"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0867223.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25732..26544
                     /gene="hcaB"
                     /locus_tag="ACHKG5_001077"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25732..26544
                     /gene="hcaB"
                     /locus_tag="ACHKG5_001077"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0867224.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26554..27756
                     /gene="hcaD"
                     /locus_tag="ACHKG5_001078"
     CDS             26554..27756
                     /gene="hcaD"
                     /locus_tag="ACHKG5_001078"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0867225.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27853..28275
                     /locus_tag="ACHKG5_001079"
     CDS             27853..28275
                     /locus_tag="ACHKG5_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0867226.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28323..29195)
                     /locus_tag="ACHKG5_001080"
     CDS             complement(28323..29195)
                     /locus_tag="ACHKG5_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0867227.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29207..30301)
                     /locus_tag="ACHKG5_001081"
                     /pseudo
     CDS             complement(29207..30301)
                     /locus_tag="ACHKG5_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30334..31332)
                     /locus_tag="ACHKG5_001082"
     CDS             complement(30334..31332)
                     /locus_tag="ACHKG5_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0867228.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31357..32868)
                     /locus_tag="ACHKG5_001083"
     CDS             complement(31357..32868)
                     /locus_tag="ACHKG5_001083"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0867229.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32891..33874)
                     /locus_tag="ACHKG5_001084"
     CDS             complement(32891..33874)
                     /locus_tag="ACHKG5_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0867230.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33971..37252)
                     /locus_tag="ACHKG5_001085"
     CDS             complement(33971..37252)
                     /locus_tag="ACHKG5_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP0867231.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37370..38563
                     /locus_tag="ACHKG5_001086"
     CDS             37370..38563
                     /locus_tag="ACHKG5_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0867232.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38626..39879)
                     /gene="glyA"
                     /locus_tag="ACHKG5_001087"
     CDS             complement(38626..39879)
                     /gene="glyA"
                     /locus_tag="ACHKG5_001087"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0867233.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40207..41397
                     /gene="hmpA"
                     /locus_tag="ACHKG5_001088"
     CDS             40207..41397
                     /gene="hmpA"
                     /locus_tag="ACHKG5_001088"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0867234.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41442..41780)
                     /gene="glnB"
                     /locus_tag="ACHKG5_001089"
     CDS             complement(41442..41780)
                     /gene="glnB"
                     /locus_tag="ACHKG5_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0867235.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41841..43175)
                     /gene="glrR"
                     /locus_tag="ACHKG5_001090"
     CDS             complement(41841..43175)
                     /gene="glrR"
                     /locus_tag="ACHKG5_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0867236.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43165..43878)
                     /gene="qseG"
                     /locus_tag="ACHKG5_001091"
     CDS             complement(43165..43878)
                     /gene="qseG"
                     /locus_tag="ACHKG5_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0867237.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44043..45470)
                     /gene="qseE"
                     /locus_tag="ACHKG5_001092"
     CDS             complement(44043..45470)
                     /gene="qseE"
                     /locus_tag="ACHKG5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0867238.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46046..49933)
                     /gene="purL"
                     /locus_tag="ACHKG5_001093"
                     /gene_synonym="purI"
     CDS             complement(46046..49933)
                     /gene="purL"
                     /locus_tag="ACHKG5_001093"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0867239.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50190..51746
                     /gene="mltF"
                     /locus_tag="ACHKG5_001094"
     CDS             50190..51746
                     /gene="mltF"
                     /locus_tag="ACHKG5_001094"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0867240.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51743..52246)
                     /gene="tadA"
                     /locus_tag="ACHKG5_001095"
     CDS             complement(51743..52246)
                     /gene="tadA"
                     /locus_tag="ACHKG5_001095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0867241.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52304..52939)
                     /gene="yfhb"
                     /locus_tag="ACHKG5_001096"
     CDS             complement(52304..52939)
                     /gene="yfhb"
                     /locus_tag="ACHKG5_001096"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0867242.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLCFCQHRWRVTPRGELQQLE"
     gene            53148..53996
                     /locus_tag="ACHKG5_001097"
     CDS             53148..53996
                     /locus_tag="ACHKG5_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0867243.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54052..54312
                     /gene="yfhL"
                     /locus_tag="ACHKG5_001098"
     CDS             54052..54312
                     /gene="yfhL"
                     /locus_tag="ACHKG5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0867244.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        12803 a        15033 c        13748 g        12736 t
ORIGIN      
        1 gaaaaatgta ctgacgcgat ttgccctacg tcatccatca ccagcaattg ataatcacct
       61 ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga
      121 aaccaccagc gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg
      181 cccggtaaac gtttaataat ctcgccatcg cgaacgcctg tcagctgcag cgggagctgg
      241 gcatcgtgac cgtatggcgg gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt
      301 tctgatgcgg gcaaccaggg ttccagcggt agcggccaga catttatcat ttcttgtcgc
      361 gcttgcgggc aatcagcggc aacacgtttg ccattttcat ccagccagat ggggaaacga
      421 atgccattaa tgccttcctg ctccggcagt aatagagttg gcggctgact cccgtccagc
      481 agccaggttg ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc
      541 ggccagcaaa taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattagca
      601 ctgcgcgaca gtaagatatt attgacctga ttcaacaatg gtacggcgct ggcaaaacca
      661 aactgaccaa caacgggcgt gccgtccggt ctgccagtcc agatcccaat gacatagcga
      721 gcgttaaccc caatcgccca ggcgtcacga tagccatagc tggtgcccgt tttccatgcc
      781 agtggggcga cgcgcggcaa ggcaccatcc ggcaagggta gcgcttcatc agccataatc
      841 cggcgaatga tccacgccgc tcccgacgac attaaaggcc gttcaagcag cggatcgtca
      901 ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa acgcggtata cgctgccgcc
      961 atatcttcca gtttcgcacc agcgccgccg agaatgagcg aaagattcgg cgcagcaccg
     1021 ttgggcaaat ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca
     1081 taggcttcca gcacctgcac agcaggtaaa ttcagcgagc gcaccagcgc ctcgctcatg
     1141 ctgatcgggc catgaaaacc gctgtcaaag ttgccaggtc ggtaatcacc ggtacgccgg
     1201 gggacgtctt gcagcagtga tgccgggtgg atcaatcctt catccagcgc cagaccgtaa
     1261 acaaacggtt tgagcaccga tcctggcgat cggatcgcat tgaccatatc aacatgacca
     1321 aagcgtgaat catcgttgag atcaaccgaa cccacccagc cgcgaacacg catatcggta
     1381 tgatcaacca cgatcatcgc cagtgagctg cgcggtggta atcgtccttt ccagttttgc
     1441 gccagttctt ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt
     1501 ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt
     1561 gccagccaga tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc
     1621 acggccatcc gttcgagcac tttattacgc gcgacttcgg cacgctccgg ccaacgatcc
     1681 gggcgaagac ggctgggcgc ttgcggcaaa accgccagca ttgccgcctc ggaatagctt
     1741 aaattcgcag gcgactttcc gagataagcc caacttgccg caccgatccc ctgcaacgta
     1801 ccgccaaacg gagcgcggtt aagatacaag gtcagaattt cacgcttaga cagatgccat
     1861 tccagttgca acgcgcgcca gagctggcga attttgccgc caaatgtttt ggggtgagga
     1921 tcaagcagac gagcaacctg catagtgagc gtgctgccac cggaaataac ccgtcccgaa
     1981 gtgagatctt gccatgctgc gcgcgccacc gagaatggat tcaccccagg atgcttccag
     2041 aaccagcgat cttcataatt gatcagcgct tcaaggtaac gtggagaaac atcttcgatt
     2101 gttacaggat aacgccagat gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc
     2161 tgcgccacga ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct
     2221 gctccccacg ctgcaagaaa aaggagaaat ggcgcagctg ccagcgttat ccagcagccg
     2281 cgtttggtta acaagcgagg catttacggt ctgacaatca gcaaatcttc agccgcgccg
     2341 gtcgcccgcc attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt
     2401 cccggcgtca ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca
     2461 accgccgcca caaagcgatc gtcacggaac tcaatgtgct taatactcgc ctgctgcatc
     2521 tggttcagta agttctgcac ttcgccacca ctttgctcca ggctggcgct accgttcgcc
     2581 agattctggt tttccagttc cagacccgca ggcagcagat ccacgactaa cgcatccggc
     2641 acgctgttac tagcttttac ctgcaaccag accagcacca gatcgccgct acgtaacgag
     2701 tccagcgatt tgctcttacc atcagtacca agaatatgac gctcaatttg cagcacattg
     2761 ttcgccggta aaggtgcgga ttgcggataa ccgctggcat ccacacgcag ccataacggc
     2821 tgctcgccac tgttggtcac ctgcaaggtg gcaagttgat cgctattcag attgctgttt
     2881 tgcgctttct cgcctgtcag cggctcagct gagaaagagg tttgcgcctg ccatttacct
     2941 ggtaaatcct gaatcgtacg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc
     3001 cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca gggagtactg ctcatcgggt
     3061 agcagtttgt tctcttccag caaggagagc atcaacgcgt tgtcgcgcag tggactaccg
     3121 taatcaccca gccatatccg ctcatcacta ttacgcggcg tttttagcgc cagcgcaatc
     3181 gcttcttcat cacgcgtcgc atcacccatg gttttcagcg caacgccaag ttgcagcagc
     3241 ggtaaaccag aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagcgga
     3301 gccttttgct gacgggccaa caccagcgca gcgtaagact gtacggcgaa tttactggct
     3361 ttgagattat ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga
     3421 cgctcattac cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg
     3481 accaggaaat ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac
     3541 agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc
     3601 gatgcacggc gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg
     3661 ttggtataaa gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac
     3721 gcttttaact ctttgatata acgtgcgatg ttcagcggtg gtttgccgct caacaacaat
     3781 tgcccttcca gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc
     3841 tcaccaggct gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg
     3901 ccgattttcc actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta
     3961 atggtggcct gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc
     4021 gtagtacgca cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc
     4081 aaaccactgg cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata
     4141 tccagcgtca gacgcgagtt atcgccactc gccataaagc gcggcatgtt cagttcagca
     4201 atcaccggtg cggcaactat cactttgctt tcgttgctac cgaattcatc tgccgtccag
     4261 gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct
     4321 tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg
     4381 accggcggtt taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc
     4441 agacgcccct gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt
     4501 ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca
     4561 ccactatcaa cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg
     4621 ctggctttaa ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc
     4681 gccagatcga ggcgacggtt ttcatcacca agcggcagat gcaacacacc aaccgcgcgt
     4741 tttggcgtcg cggagcgaga tttatcgcca ggacgcacca ccagcgtact caaataaaga
     4801 tcatgacgat tccaggtttt atcgaccgga atcgtcagat ccagcccttg agccggaaca
     4861 tcaatctctt gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc
     4921 gccgttggcg cggcgatatg caacttaatg gtatcgccag ggcgataacc ggctttatcc
     4981 agtttcagcg tgacacggtc gggtcgcact gcgccgctac cgtcgctatt atcctgccag
     5041 ctatagccag cccaaaaacg aacactactg accgcttcat tcggcgcttt gacttccaga
     5101 cgataagcgc cccactctac cgggaaactg accttgccgg tttcgtccgc tttcagatcc
     5161 agagtttgtt cattttcaat cagatctttt tgatcaaact gtgattgcca gccttcatct
     5221 tctgaccagt tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac
     5281 acggcttttt tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct
     5341 tcatcaacaa tgggctgttt caccgtgctg tcagtgcgat aatcgtaaac cgatttcgag
     5401 gcgaactgcg gacggatccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg
     5461 cgcgtcaccg ggcgaccgcc cgattccagc aaactgccct ggaaaataac ctgcaatggg
     5521 gaatgcgttt ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc
     5581 gtcaactgaa cttcatccag cgtgcgggaa aggttttcgg cagctatatc gccgaattca
     5641 aaaccaggta aggctgacac agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa
     5701 gtattaccat tagcaggcgc accgtacagg tagtatccca ccacggagaa tttcacttca
     5761 tcattcggcg taagcggggt tttctcaccg gtcagattca gcgccatacg ctctggcata
     5821 aaatcttcga cgtggaaatc ccacatccga tactggttat cgcccgtgtt agcgcgaata
     5881 tgccacatac cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattt
     5941 tccggctgac taacgacgct cctgagtacc tgcccatcgg gtttaatcac gtctaacttg
     6001 atgggttgat tgggcaacgc tttaccgtct gcatcacgca gcaaaccatt gaggattacc
     6061 gtttcacccg ggcgataaag atcgcgcggg ccaaacatga aaaactgttt gctatagcct
     6121 ggcgcgccag caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc
     6181 gttgtctgac cgtctttacg tgccagcaat aatgcggcgt ttttatcatt ttccagctgc
     6241 acatgcccct gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa
     6301 gagacttcaa ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga
     6361 cgattgtgat aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga
     6421 ttactgtaat cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga
     6481 agcggtttga tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg
     6541 agatcaaacc gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag
     6601 ttcgccagcg aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta
     6661 acgcggaaga agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca
     6721 acgactttac caggcagcag cgaaccacgg ctggcaaaac cgacactggg ttggatgtcg
     6781 cgggtagtta tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc
     6841 tccttgccaa ttgtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgcagctct
     6901 ttaagattat ctgatagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca
     6961 tgaataacgc gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc
     7021 gtggctgcac catcaagttg gacttcagag agatccagca atgtcagcgc cttaccggca
     7081 ctctgttggg ccagtttttg tcgctctggc gcggagagtt ttgctgaact cgcgttttct
     7141 gaagaggccg ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta
     7201 tcgttgttgt cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt
     7261 ttcatttttc atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga
     7321 gaatttacgt tctgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt
     7381 ggctcagaca gccaaatcag cctcttttgc ttgtcgcttt tcactaaaca tccgacaatt
     7441 tagcctaacc ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga
     7501 ctggctcgcc gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc
     7561 gcctggacag gaagatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc
     7621 agtgtttttt gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc
     7681 gcgcccggaa acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct
     7741 gattgtctat gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac
     7801 ctttggtgta gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga
     7861 tctgttgtta gaagaaggtg cagtagagct gccggaaggt gagtttaacg ccgcgtttaa
     7921 tcctgaagca gtggtgaaag taaccgatgt gttattggca agccatgaaa atacggcgca
     7981 aattattgat gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg
     8041 tttacgtcgc ggacatattc ccggtgcatt gaacgttccg tggacggaac tggtgcgcga
     8101 aggcgaacta aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta
     8161 tgacaaacca attatcgtca gctgcggctc tggtgtaaca gcagccgtgg ttttgttggc
     8221 actcgcgacg ctggatgtgc caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg
     8281 cgcgcgggca gatttaccgg ttgagccatt gaaataagct ttttacaggc aataaaaaac
     8341 cgccgaattt ggcggttttt tattgctggt ctggttcgcg gcctttccag caggttgact
     8401 tgtgttgccg gagcaacgca ggcaccccac agtgttgaaa tactctcttt tatgcctggc
     8461 tgtgtcaaga taataaacag agtaattatt caaacatgag agtattattc ttaatggccc
     8521 ttttcgccgt ctcgcaaacg ggcgctggct ttaggaaaga atgttccatg accgtaaata
     8581 caggcacccc acagtgcttg ctatcgcggc aatatcgcca gtggtgctgt cgtgatgcgg
     8641 tcttcgcatg gaccgcacaa tgaagatacg gtgcttctgt atcgtactta ttgtttctgg
     8701 tgcgctgtta accgaggtaa ataataaccg gagtctctcc ggcgacaatt tactggtgat
     8761 taacaacctt cagagcagca ggtaagcccg aatgccgccc tttggggcgg catattttag
     8821 attatccgat tctgtttaaa gtcacgcaaa aaaccacccc agcgacgttc atagaatggc
     8881 gcaatatgtt cggtaataaa gtggctaatt cctttttccc cttttttcac ctgacaaata
     8941 tcgattggtt catcgccagg taatgtatcg gtcgctacac ttcccgccac ctgaataatt
     9001 tcttcgatat caccatcggc ttcaatgcca ataagtaaat taggctgtgc ctcttcgttc
     9061 tctttaattg aacaaataaa agcacgcttc acaggcttaa tggttttaaa taaggtggtg
     9121 agtgaatcaa tcatttgtgc tggcggctct gcgacttccg ataatatcag cgattcaccg
     9181 ccttccagga tttcctggct gctcagcgga tttccctctt caccaatcaa caaactgatt
     9241 tcacgcggca taaattcttt accggtcggc agtttggcat taaggaagag cgtttcgcca
     9301 agggtcatct caaacaatgt gcgaacgggc attacgacaa atgcctgttc gtcttcaacc
     9361 gcctgttgaa gtgcttctaa cgaggtaaaa aaaggaatga cgctggtgcc gtcttctttt
     9421 tcccagtgct gtaaatcaag cgcgctatct tcaaccacag cctcgccctg cgccgccgta
     9481 ccaggcaccc agacggtgga ttccagtaga gtacggaaaa aggccgggcg gtgcgccggt
     9541 tcggttgctg ctttttccag caggtcttca agttcatttt ttgtttcgga cataagaacc
     9601 acaattcatt caacgttcgg ggcacaagtg cctgatgcgc catacttatc aggcctacaa
     9661 gggattcaca atttgttgaa tttgcaatac tttgtaggcc ggataaggcg tttacgccgc
     9721 atccggcata ttagtttacg ccgttaacag attagcaatc gtgcgcacac caagtcccgt
     9781 agcacccgca gaccactgtt caaccggcgc tttacggtaa gtcgccgagc agtcgatatg
     9841 cagccagcct tgctgatagt tctcaacaaa gtgcgacagg aagcccgctg ccgtgctcgc
     9901 gcctgccgga tacgccgcgc ttccggtatt gttcagttcg gcaaagttag acggcagctg
     9961 gctgcggtgg aattccgcca gcggcagacg ccagaatggt tcgttctctt gtgaggcact
    10021 cgccagcaaa cgaccggcaa gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc
    10081 cagcgcagtt ttcgccgccc cggtgagggt cgccgcatca atgatcattt ccggtttctg
    10141 cgcactggca tcaatcagac catcggcaag caccaggcgc ccttcggcat cagtgttcat
    10201 cacttcaact tttttaccgt tgcgataagt gatgatatcg cccagcttga acgcattgcc
    10261 gctaatcagg ttatccgcac agcagaggaa cagcttcacg cgcttgttca gtccgcgcgt
    10321 aatggcaaat gccagcgccc cggtaaccgt tgccgcgccg cccatgtccg acttcatcga
    10381 gtccataaac gcagtctgtt tgatgctgta gccgccggag tcaaaagtga tacctttacc
    10441 taccaggcac gcgtacactg gcgcttcttt atcgccagtt gggttgtaat ccagcgccag
    10501 caataccgga gaacgttctg aaccgcgtcc gacggtgtgc agccccatat aaccttgctc
    10561 acgcagatct tcgcctttgg tgatccgata agtcacacga tcgctcgcga cgttgctgat
    10621 cagatcaaca gcacgctgtg ccagttgcga tggtcccaat tcttctgccg gtgcgttgat
    10681 ggtgtcacgc acccagtcga tgatcatcag gcggttatcc agttcctggc gctgggcatc
    10741 gtccagatcc ggccactcca ctttacgcgt gcctttcggg gctttgtaac cttgccagaa
    10801 tgcccagcat cgatccgcat cccagccttc accgcttaac tgaacatgct tgatgcccag
    10861 accgtcaatc ttgcgcgccg cacgctggat cagccccaga tcgtctgccc cgttcaaatg
    10921 cagggtaatg ccgtcattat taatgctgta agttgctttt tctccccagc gcgcgtcggc
    10981 aggttgggta gagagggtaa tcttcatcgc ttctgtcatt ttagttatcc ttctttgtaa
    11041 aagggccgcc agttggcagc cccaggattt ttccgctaaa tgattcgcgt tgcaggaagg
    11101 cggcaagtga gtgaagcccc aggagcatag ataactatgt gactggggtg aacgagcgca
    11161 gccaacgcat ctgcggcgtg aagcatgacg cggaaattat tcggcctcgt ccagccagac
    11221 taacaaaatc gcttcgagga ttttctcgtt ggatgcctgc gggtcgtcgt cgaaatcttc
    11281 cagatcgcaa atccactgat gcatatcggt gaatcgaacc gttttcggat caagatcggg
    11341 atacgcatcg tacagtgctt cgccaatttc gcggctatcg gtccacttaa gtcccatact
    11401 aacctctgtt aatgctcacg cgcatggttg atcgtgtaac gcgggatttc gaccactaaa
    11461 tcttcgtcgg tgacgcgcgc ctggcagctt aaacggcttt ccggctccag tccccaggct
    11521 ttgtccagca tgtcgtcttc ctgctctgag ctttctggaa gcgagtcaaa accttcacga
    11581 acgatgcagt ggcaggtggt gcaagcacag gatttttcac aggcgtgttc aatctcgata
    11641 ccgttacgca gagctgcgtc gagaatggtt tcaccgctat tagcttccag aacagcgcca
    11701 tcagggcaga gatcctgatg aggcaaaata acaatctttg gcatattaaa cctcgtccac
    11761 ggaatggcct ttcagcgcac gacgaaccga ctggtccatg cggcgagcgg cgaaatcctg
    11821 ggtttgtttg tctacgtttt taatcgcttg ttcgatggcg tcaacgtcat cgccctgcgc
    11881 cacttcactc aggtgagcgg cagcatcgtc aatgacctga cgttctgcgg cgcttaacag
    11941 cgcggcatca gcagccagcg cgccgtgcag actttccagc acacgcgccg cttctacttt
    12001 ttgttctgcc agcattcggg cttttacgtc ctgctcggca tagctcattg agtctttgat
    12061 catcgaagcg atttcgctat cggtcagacc gtaagacggt ttgacctgaa tagacgcctc
    12121 aacgccggtg gatttctcca tcgccgtcac gctcaaaaga ccgtcggcat cgacctggaa
    12181 cgtcacgcga atatgcgcac cgccagccgg tagcgccgga ataccacgca gcgcaaaacg
    12241 cgccagtgag cggcagtcct gcaccagttc gcgctcaccc tgcattacat ggatagacat
    12301 cgccgtctga ccatctttaa aggtggtgaa atcctgagcg cgggccactg gaatagtggt
    12361 attacgcgga atcactttct ccaccaggcc gcccatcgtt tcgaggccca gcgacagtgg
    12421 gatcacatca agcaacagca tttcgctgtc tggcttgtta cccaccagaa tatccgcctg
    12481 aatcgccgcg ccaatagcga cgactttatc cgggtcgatg gaagtcagcg gtggacgacc
    12541 gaaaaattcg cctacccgtt cacgcaccag cggcacgcga gtagaaccgc ccaccatcac
    12601 cacttccagc acttcatcag cttctacacc cgcgtctttc agcgcgcgac gacaagccaa
    12661 taaggttcgt tttaccagtg gcgcgatcag ttcattgaat tgttcacggc tgatttcgcc
    12721 ctgccagccc gcaacgttaa cggtcacgga gtccgcatcg ctcagcgcga ttttggctgc
    12781 aatggcggca tccagcagtt cacgctgaac gcggttatcg ctacgatcag gaatgcccgc
    12841 ctgctcgcga atgtaatccg ccagcagatg gtcgaaatca tcgccgccga gcgcggaatc
    12901 accgccggtt gccagcactt caaacacgcc gcgacttaag cgcagaatgg aaatatcaaa
    12961 cgtcccgcca ccgaggtcat aaacggcgat cacgccttcc tgaccggaat ccagcccgta
    13021 ggcgatagcc gcagcggtcg gttcgttaag taagcgcagg acgtgaaggc ccgccagacg
    13081 cgccgcgtct ttggtgccct gacgctgggc atcgtcaaag tacgccggaa cggtgataac
    13141 tacaccatcc agctcgcctg ccagggcttc agttgcccgc gccgccagtg ctttgaggat
    13201 gtccgcagaa acgcgcaccg ggttcagcag ccccgccgcc gtttcaatca tcggcaggcc
    13261 gttttcgctg gcctggaatt gataaggcag atgcggatag cgttgctgga tatcagccag
    13321 cgagcgtccc atcaggcgtt taacagaact aattgtgttg gcggtatcga gcgctgcatt
    13381 agtacgcgcg tcataaccca ccgaatgccc ttgctgttga tagtgaacaa cagatggcag
    13441 caggtgacgg ccttcatggt cggctaacgt ttcggcctga ccgctgcgca ctgtcgccac
    13501 cagcgagttg gttgtgccca ggtcaatacc ggccgccaga cgacgctgat gcggcgcagc
    13561 actcaaacca ggttcactaa tttgtaataa ggccatgttt agcttccaga aattaaaaat
    13621 cgagcagttt ttcttcgagt tgttcggcac tgcttcgcag tttatcgaga aaacgcagct
    13681 tacgcacggt atccgccgcc gcgtcccagg tctcgttgtc taactgttca accatcaact
    13741 gatggcgggt atcaaacatc tttttcacac gtttgataaa gctttccagc cgcgcttcat
    13801 ctttcgcctg ttcgatctcg tccagctctt cgcgcagctc caactgttcc atcaggaacg
    13861 cggtgtcgcg cacagtatgc tgctcgctgg cgagatcaaa gccgtgcaaa gaaagcaaat
    13921 attccgcgcg cattaacgga tgacgcagcg tttgccaggc ctgattaatg gttgcagatt
    13981 gctgtacggc ggcgagttgt tccgcctggc ttccgctggc gaatttatca ggatgatact
    14041 gacgttgtag atcctgaaaa cgcaggctca gcgcctgggt atcgagttga taacgggcag
    14101 gcaagccaaa gagggtgaag taatccataa cattctcagg gctgcgatcc gccgcgataa
    14161 cggcgaatca ggtaaaacaa cccccacgcg caggcgacca cggtggggtt atcggtatgc
    14221 gcatcaaacg tggaagcttt cgccgcaacc acactcatct ttaacgttcg ggttggtgaa
    14281 tttaaaccct tcgttcaggc cttcttttac gaagtccagc tgcgtaccgt ccagaaattg
    14341 caggcttttg ccatcgacca cgactttcac gcctttgtct tcaaacacga tgtcttccgg
    14401 cgtcggttcg tcaacaaatt ccagtacata agccatacct gaacatccgg aggttctcac
    14461 gcccagacgt aggccaaacc ctttgccacg gttagccaga aaggtattta ctcgcgctgc
    14521 tgcactgtcg ctaagtgtaa tcgacataac caaacctcaa ctcttatttt gcttcacgtt
    14581 tgcttttata gtccgcaatg gcggctttga tcgcgtcttc tgccagaata gaacagtgaa
    14641 ttttcaccgg cggcagttca agttcttcag caatatcggt gtttttgata gcctgcgctt
    14701 cgtcgagaga cttccctttc acccattcgg tgaccaggga gctggaagcg atagcagaac
    14761 cgcagccgta agttttaaaa cgcgcgtctt caatgatacc ttcatcgttg actttaatct
    14821 gcaacttcat tacgtcgcca caggccggtg cacccaccat gccgctgccg acgttctcgt
    14881 cgttgttgtc aaaggaaccc acgttacgcg gattctcgta atggtcgata actttttcgc
    14941 tgtaagccat tataaattct cctgattccg ataccgatta atgatgagcc cattcgatgc
    15001 tgttcagatc cacgccctgc ttgtacattt cccacagcgg agaaaggtca cgcagacgac
    15061 cgatggattt acgaactaac tcgatggtgt agtcgatctc ttcttcagta gtaaaacgac
    15121 ctaaagagaa acggatagag ctatgtgcca gctcgtcgtt cagccccagc gcgcgcagca
    15181 cgtaggacgg ttcgaggctt gctgacgtac aggcggaacc ggaagaaacc gcgagatctt
    15241 tcagcgccat aatcagcgac tcaccttcaa cgtagttgaa gctgacgttg agaatgttcg
    15301 gcgcaccgtg ttccaggtca ccgttcaggt aaacttcttc gatatctttg atgccgttcc
    15361 acagacggtt acgcaggccg cgcagacgtt ccatctcggt cgccatctct tcttttgcga
    15421 tgcgataggc ttcgcccatt ccgacgatct ggtgaacagg cagagtgccg gaacgcatac
    15481 cgcgctcgtg accgccgccg tgcatttgcg cttcgatgcg tacgcgcggt ttacgacgca
    15541 catacagcgc accgatacct ttcgggccat agattttgtg accggagaaa gacatcaggt
    15601 caactttcaa ctggctcagg tcgataggca gtttgcccac gctctgggtt gcatcaacgt
    15661 gatagataat gccacgagca cggcacattt cgccgatagc cgcgatatcc tgcaccacgc
    15721 cgatttcgtt atttacgtgc atgatagaaa caaggatggt gtcgtcacgc atcgctgctt
    15781 cgagttcttt caggtcgata ataccgttac gctgcggtgc gaggtaggtg acttcgaaac
    15841 cttcgcgctc cagctgacgg caggtatcca ataccgcttt gtgttcggtt ttgctggtga
    15901 ttatgtgctt gccttttttc tgataaaagt tggctgcacc tttgatcgcc aggttgtcag
    15961 actcggttgc accagaggta aagacgattt cacgcggatc agcgccgacc agatcggcaa
    16021 tctgattacg ggcgatatct accgcttctt cagcctgcca gccgaaacgg tgagaacggg
    16081 aggccgggtt accaaaggtt ccgtccatcg tcataaactg catcattttc tcggcaacac
    16141 gcgggtccac cggcgtggtt gcggagtagt cgagataaat cggtaatttc attgctctat
    16201 aaactccgta catcactcaa tgcaaggaac caggctaccg gctggatgta cgaccgtgtt
    16261 tacggagcgc gatacgcccc ggcctgattc tgaatacttt ttattaagcg cgtaacttaa
    16321 cgtcgatcgc gtcttgtgtg cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca
    16381 catccagcac ttcctggttg ttaaccagtt cgcctaaagt aatgttattg agaaaaccgg
    16441 taagacggtc gctcaaatca cgccacagcg cgtgggtcag gcatttatcg ccgccctggc
    16501 agccgccttt accctgacaa cgggtggcat ctacagattc gtcaacggcg ctaatcactt
    16561 cgccaacggc gatgctgctg gcatctttgc ctaacagata accaccgcct ggtccacgta
    16621 cgctggaaac cagaccattt ttacgcagac gagaaaacag ttgttccaga taagaaaggg
    16681 aaattccctg acgttcggaa atatcagcca acggtaccgg gcccgcttca gagttgagcg
    16741 caacgtcaag cattgcggtc acggcatagc gccctttaga tgtcagtctc atgtcttact
    16801 tcacctcaaa ctcgcccctg cccggggttt tttattgtaa aagtgggggt attgcatagc
    16861 agggtcaagt ctgacattcc cgagtaaatt ggtcaactat ttacttgact gatttagtcg
    16921 ggtatttaac cttcagtgcc atttttttat cgtggcgttt gtacctgtta tccttccaga
    16981 ccatgggcgg gttgagggca attattcgca ttagcgcagg cctgttcccc tcaccctaac
    17041 cctctccccg aaggggcgag gggaccgtcc gtgcacttat ctggcttagc cctcaggcct
    17101 tattaccttt attctgctgc tctatagaag ccagaatccc gcgcaggata ttcaattcct
    17161 ggctttccgg gcgcgcacgg gtaaacagac ggcgcaattt attcatcacc tgccccggat
    17221 ggttttcacg gataaaaccg gttgccagca gcgtttgttc cagatgacca taaaaacgct
    17281 ccagatcatc gaccagcgga tacggcgtct cttcatgctc gacctgctcg ccattttcct
    17341 gagttgccag ccaggccata cgcacttcat aggcaataac ctgaaccgcc atcgccaggt
    17401 tcagcgagct gtattcaggg ttagcggcaa tcgcgacatg ataatgacac ttctgcaact
    17461 cttcattggt caggccgaca cgttcgcgac caaacaccag cgccaccggg gtatttgccg
    17521 cttcagcaac gcttttcaga ccgcattcgc gcgggtcgag catcggccac ggcagcgtgc
    17581 gggaacgtgc gctggtgccc accaccaggc tacaaccggc taacgcttcg tcgagcgtat
    17641 cgacgatgtg agcattacca atcacatcgc tggcccccgc cgccagggca atcgcctggg
    17701 agtcaggttt caccagtgga ttaaccagcc acagattggt taatcccatt gttttcatgg
    17761 cacgggcaac agaacccata ttgccggtgt gtgacgtctc caccagcaca attcgaatat
    17821 tttgcagcat tgtctttctt cgtctaaaga ttattcgcgc atcttatcat aaaacgaaga
    17881 cagatgccga tctcgctgct atactctgcg ccgttttccc gttctttaac atccagtgag
    17941 agagaccgat gcatccgatg ctgaacatcg ccgtgcgcgc agcgcgcaag gcgggtaatt
    18001 tgattgccaa aaactatgaa accccggacg ctgtagaagc gagccagaaa ggcagtaacg
    18061 acttcgtgac caacgtagat aaagctgccg aagcggtgat tatcgacacg attcgtaaat
    18121 cttacccaca gcacaccatc atcaccgaag aaagcggtga acttgaaggt actgatcagg
    18181 atgttcaatg ggttatcgat ccactggatg gcactaccaa ctttatcaaa cgtctgccgc
    18241 acttcgcggt atctatcgcc gttcgtatca aaggccgcac cgaagttgct gtggtatacg
    18301 atcctatgcg taacgaactg ttcaccgcca ctcgcggtca gggcgcacag ctgaacggct
    18361 accgtctgcg cggcagcacc gctcgcgatc tcgacggtac tattctggcg accggcttcc
    18421 cgttcaaagc aaaacagtac gccactacct acatcaacat cgtcggcaaa ctgttcaacg
    18481 aatgtgcaga cttccgtcgt accggttctg cggcgctgga tctggcttac gtcgctgcgg
    18541 gtcgtgttga cggtttcttt gaaatcggtc tgcgcccgtg ggacttcgct gcaggcgagc
    18601 tgcttgttcg tgaagcgggc ggcatcgtca gcgacttcac cggtggtcat aactatatgc
    18661 tgaccggtaa catcgttgct ggtaacccgc gtgttgttaa agccatgctg gcgaacatgc
    18721 gtgacgagtt aagcgacgct ctgaagcgtt aatgactcag gcgggtgata tcactcaccc
    18781 gccctcgcct ttcaggcgct attccgaaat acttcctcac cgctttactt tctttcgtca
    18841 ctctcccacc atctttcccc gattaatgga tgataaagaa gtcgcaagga aaggttatgg
    18901 cactgccagt gaacaaacgc gttcccaaaa ttctgtttat tctctttgtt gttgccttct
    18961 gcgtttattt agtgccgcgc gttgccatca acttcttcta ttatcctgac gataaaattt
    19021 acggtcccga cccctggtcg gcggaatccg tcgaatttac ggctaaggac ggtactcgtc
    19081 tgcaaggctg gtttattcct tcttcgacgg gccctgctga caacgccatc gcaaccatca
    19141 ttcatgctca cggcaatgcc ggaaatatgt ccgcccactg gccgctggtc agttggttac
    19201 ccgagcgtaa tttcaacgtt tttatgtttg attatcgcgg gtttggtaaa tcaaaaggca
    19261 cgccgtccca ggccggattg ctggacgata cgcaaagcgc catcaatgtg gtgcgccatc
    19321 gcagtgatgt aaacccacaa cggctggtgc tgttcgggca gagtattggc ggggcgaata
    19381 ttcttgctgt tattggtcag ggtgatcgtg aaggcatacg tgcagtgatc ctcgactcca
    19441 catttgcctc ttatgcaacc atcgccaacc agatgatccc cggcagtggc tacttacttg
    19501 atgagagtta cagcggcgaa aattatatcg ccagcgtcag cccgatcccg cttttactca
    19561 ttcacggtaa agctgatcac gttatcccat ggcagcacag cgaaaagttg tatagcctgg
    19621 caaaagagcc aaaacggttg atcctaatcc cggatggcga acacattgat gctttttccg
    19681 atcgtcacgg cgacgtttat cgcgaacaga tggtgaactt tatccttagc gcgttgaatc
    19741 cgcagaacta acccatgatc gctagcacga taatcattca caaaatcacc ttaagacatg
    19801 ctaatccact ggtcaggaca gtttaagatg agaaaaattc tgtgacgctt gccaacattt
    19861 ctgatgatta gcattccctt cgccatttcc ctgagcaaac tttagctatt cttatcaatt
    19921 atgcttatgg gagatctaca gatgatgcct acgcttgctc caccatctgt cctttcggct
    19981 ccccagcgcc gctgccagat cttgctgacg ctctttcagc cggggttgac cgccaccatg
    20041 gcaaccttca gcgagcttaa tggtgtggat gatgatattg ccagtcttga tatcagcgaa
    20101 acaggacggg agatcctgcg ctatcatcaa ctcacactga cgactggtta tgacggtagc
    20161 taccgggttg aaggtacagt gcttaaccaa cgtttgtgtt tatttcactg gctacgacgt
    20221 ggtttccgtc tgtgtccgtc atttattacc agccaattca cccccgccct gaagagtgaa
    20281 ctgaagcggc gcggaattgc gcgtaacttt tacgacgata ccaatctaca agcgttagtg
    20341 aatctctgct cccgacggct gcaaaaacgc tttgaatcgc gcgatattca tttcctgtgt
    20401 ctgtatctgc aatattgttt gctgcagcac cacgctggaa ttacgcctca gtttaatccg
    20461 ctccaacgtc gctgggcgga atcctgcctt gaatttcagg tagcgcagga aattggacgc
    20521 cactggcagc gtcgggcgct ccagcctgta ccacctgatg agccactgtt tatggcacta
    20581 cttttttcca tgttgcgggt tcccgatcca ttgcgggatg cgcatcagcg ggacagacaa
    20641 ttgcgtcagt ctatcaaacg tctggtaaac cattttcgtg agctgggaaa tgttcgtttt
    20701 tatgatgaac aggggttatg tgatcagctt tatacccacc tcgcccaggc gttaaatcgc
    20761 agtttgtttg ccatcggtat tgataatacc ctgccggaag agttcgccag actgtatcca
    20821 cgcctggtac gcaccacccg cgcggcgctg gccggatttg aaagtgaata cggcgtccat
    20881 ctttctgatg aggaaagtgg tctggtcgcg gtgattttcg gtgcctggct aatgcaggaa
    20941 aacgatctgc atgaaaaaca gattattcta ctgaccggga atgatagcga gcgagaagcg
    21001 cagattgagc aacagttacg cgaactaacg ttactgccgc tcaacattaa acatatgtcg
    21061 gtaaaggcat ttttgcagac aggcgctccg cgtggcgcgg cactgattat tgctccttat
    21121 accatgccgt taccgctctt ttcaccaccg ctgatctata cggacctgac actgacaaca
    21181 catcaacagg agcagatccg caaaatgctc gaatcagcat gagggaacaa ctttcgggcg
    21241 caaaaacatt gccggaagcg ccaccagcgc catcacccag aacacgccgt ggcccagata
    21301 ttgatacagg aaaccggcga aaacggtcat gatagcgata ctgccgccca tcgcgacggc
    21361 agagtaaacc gcctgtaaac ggatgacttc gctaccctgg cgagcagcaa tatagcgcat
    21421 ggcggccagg tggcagaccg tgaaggtgcc gcaatgcaga atttgcacca ctatcaacca
    21481 cggcaacgcc gtagttgctc ccataatgcc ccagcgcact acgccgcaaa tcgctgagat
    21541 caacagcata tcgcgtgcac tacaacggcg gaaaagttta ttactcagtg caaagataat
    21601 gacttccgcc accacgccca gcgaccacaa ataccccacc gccgaggccg agtagccagc
    21661 tgcctgccag taaatggcgc taaaaccgta ataggccgca tgtgccccct gcaataaaca
    21721 aacgcaggcc agaaagcgcc agttctggcg aaccagcgcc aaccacgctg accaaccggt
    21781 gctctcctgc tggcggcttg ccccttgtgg ctgaatcgtc ggacggatga gaaagccgag
    21841 cagcatggat gccacgccca acgtcaacag cacgaggatc acccgataat caaacatagt
    21901 gaccagtttg cccgtcagcg ccgagccaat gacaaacgcc accgagcccc acagtcgcac
    21961 tttgccgtaa tcaagcggga actgcttttg ccacgtattc gccagtgcat cggtcaacgg
    22021 taccagcggt gagaaaaaga ggttaaagcc aatcatcacc agcatcagcc acgctacgtg
    22081 cgcccccgcc cagaaggcga cagcaaagag aagtgtcagc agtgccagca cgcgcaaggc
    22141 ggaaatcagg cgagaaggat cgctgacgcg gggcgcgatg agcaaactcc cgagaaaacg
    22201 ggcaaccaga cctgccccca ataacaggcc gatggtttct ggcgttaaac caatcccttt
    22261 aagccagacg ctccagaaag gtagaaaaat gccgtaacta aaaaagtatg tgaaatagcc
    22321 gagcgccaac cagcgcgtgg attgcaaaac catgagtccc tcccgtcagg aggcgttagt
    22381 ctggagttaa tctaatgttg gtgcaagttg aaaaataaga atattcgagc ctgcttcccg
    22441 atgtctgtaa cgccagttca caagcactca aaaactgcga tccgtgacgc atgttgcaaa
    22501 gataatgcgt aattatgccg ttacgcttgc caaacgttcc tgcacaatgg cgatgaaatc
    22561 gcgtaatgcc ggcttcatct cccctttctt ccacgccatc agtaaagcaa tggaaggcac
    22621 atttccggca atgggacgaa aaacaacctg cccggtatta aaattattca tataaccggg
    22681 tatcaaagtg acgcccagcc ccatgcccac cagattcatg gtcaccagaa tattcgttgc
    22741 cacctggacg atatttggct ggctgttttc ttgtgcaaac cacgctttaa cgatcggcgc
    22801 aagcgaaccg gaatacgccg gatcggtact gacgaaattc acgccatcca gttgcgcggc
    22861 ggtgatctct ttttcatgtg ctaaagggtg atcaaccggt aacaccacca ctaatggttc
    22921 gtcaaaaagc tccagataat caatctccgg gctataaaca ggatggcgca ttaagccgac
    22981 gtcgagttca ccacggcgaa ttttttcctc ctgttgcgtg gtgattaaac tcaccagctc
    23041 aatcaaggtg tctggctgtc tgagacgaaa catcggtaat acttttggca gtaaattcac
    23101 ttccgccgat ggcacaaagc caatggttaa ttgtctgtct tcctgaacaa ttttccgcgc
    23161 ccgtaatttg gcattttccg cttgttcgag gattgccagc gcgtcctgga gaaaacattc
    23221 tcccgccgcc gtcaacgcga ctttgcgctt atccctcacc agtaacggaa caccgacaca
    23281 gttttcaaga tcgcggatct ggctgcttaa cgaaggctgt gaggtatgca gtttttccgc
    23341 cgcacgggta aagttcagtg cctgcgccac tgcgacgaaa tagcgtaaat gccgtagttc
    23401 catcaccttc cccttgttat cgaaaaaacg tctcagctgg tagaaattaa ctatttcaca
    23461 tattagcaac caaccagcaa catccttatg acacaaaaat agaaggtcaa tacatcttat
    23521 ctttcaggat taaaaaatat gaccacaccc tcagatttga acatttacca actgattgac
    23581 acccaaaacg gtcgggtcac tccgcgtatt tataccgacc cggacattta ccaactggag
    23641 cttgagcgta ttttcggtcg ttgctggcta tttctcgccc acgaaagcca gatcccaaaa
    23701 cccggtgatt tctttaacac ctacatggga gaagatgcag ttgtcgtggt gcgccagaaa
    23761 gacggcagca tcaaggcgtt tctcaaccaa tgccgccacc gggccatgcg tgtgagttat
    23821 gcagattgcg gcaacactcg cgcctttacc tgcccgtatc acggctggtc ttatggcatt
    23881 aacggcgagt tgatcgatgt accgctggaa cctcgcgcct acccacaagg gttgtgtaaa
    23941 tcccactggg ggctaaacga agttccttgt gtggagagtt ataaagggct gatttttggc
    24001 aactgggata ccagcgcacc gggcctgcgt gattacctgg gtgacattgc ctggtatctg
    24061 gatggcatgc tggatcgtcg cgaaggcggc accgaaattg tcggcggcgt acaaaagtgg
    24121 gtgatcaact gcaactggaa attcccggca gagcagttcg ccagtgacca gtatcatgct
    24181 ctgttcagcc atgcttctgc cgttcaggta ttaggggcga aagatgatgg cagcgataag
    24241 cgcctcggtg atggacaaac cgcccgcccg gtgtgggaaa ccgccaaaga tgccctgcaa
    24301 tttggtcagg atggtcacgg cagcggcttc ttctttactg aaaaaccaga tgctaatgtc
    24361 tgggtcgatg gcgcagtttc cagctattac cgcgaaacct atgccgaagc tgaacaacgt
    24421 ttaggtgaag ttcgcgccct gcgcctggcg ggtcataaca atattttccc gacactttcc
    24481 tggctcaacg gcactgccac actccgcgtc tggcatccgc gcggccctga tcaagttgaa
    24541 gtgtgggcgt tctgtattac tgacaaagcc gcctccgatg aagttaaagc cgcttttgaa
    24601 aacagcgcca ctcgtgcttt tggtcctgct ggttttctcg agcaggatga ctcggagaac
    24661 tggtgtgaaa tccagaaatt gcttaaaggc caccgcgccc gcaacagcaa actgtgtctg
    24721 gaaatggggc ttggtcagga aaagcgccgc gacgacggca ttcctggcat tactaactat
    24781 atcttttcag aaactgccgc tcgtggaatg taccaacgct gggccgatct tctgagtagc
    24841 gaaagctggc aggaagtgct cgataaaacc gccgcttacc agcaggaggt gatgaaatga
    24901 gtgcgcaagt ttcactagag ttacatcacc gcattagcca gtttctcttt cacgaagcca
    24961 gcttactgga cgactggaaa tttcgtgact ggctggcaca gctcgacgaa gagatttgtt
    25021 acaccatgcg caccacggtg aacgcgcaaa cgcgcgaccg ccgcaaaggt gttcagccgc
    25081 cgacaacctg gatttttaat gacaccaaag accagctgga gcggcgaatc gcccgtctgg
    25141 aaacgggcat ggcatgggca gaagagccgc cgtcacgcac ccgccactta atcagcaact
    25201 gccagataag cgaaaccgac atcccaaacg tatttgctgt tcgggtgaat tatctgcttt
    25261 atcgggcaca aaaagagcgc gatgaaacgt tctatgtcgg gactcgtttt gacaaagttc
    25321 gccgtctgga agatgacaac tggcgcttgc tggaacggga tatcgtcctg gatcaagcgg
    25381 taatcacttc ccataacctg agtgtactgt tctgatgaat cgaatttatg cgtgtcccgt
    25441 tgcggatgtg ccggagggtg aggctctccg gatcgatacc tcgcccgtca tcgccttgtt
    25501 caacgttggc ggcgagtttt atgccattaa cgatcgttgc agccatggta atgcgtcgat
    25561 gtcagaaggg tatctggaag atgacgccac ggtggagtgc ccgctacacg ccgccagttt
    25621 ttgcctgaaa acggggaaag cgttatgcct gcccgccacc gatccgctca ccacttatcc
    25681 agtacacgtt gaaggtggtg acattttcat cgacttaccg gaggcgcagc catgagcgat
    25741 ctgcataacg agtccatttt tattaccggc ggcggatcgg gattagggct ggcgctggtc
    25801 gagcgattta tcgaagaagg cgcgcaggtt gccacgctgg aactgtcggc ggcgaaagtc
    25861 gccagtctgc gtcagcgatt tggtgaacat attctggcgg tggaaggcaa cgtgacctgt
    25921 tatgccgatt atcaacgcgc ggtcgatcag atcctgactc gttccggcaa gctggattgt
    25981 tttatcggca atgcgggcat ctgggatcac aatgcctcac tggttaatac tcccgcagag
    26041 acgctcgaaa ccggcttcca cgagctgttt aacgtcaacg tactcggtta cctgctgggt
    26101 gcaaaagcct gcgctccggc gttaatcgcc ggtgaaggca gcatgatttt cacactatca
    26161 aatgccgcct ggtatcccgg cggcggtggc ccgctgtaca ccgccagtaa acatgccgca
    26221 accgggctta ttcgccaact ggcttatgaa ctggctccga aagtacgggt gaatggcgtc
    26281 ggcccttgtg gtatggccag cgacctgcgc ggcccacagg cgctcgggca aagtgaaacc
    26341 tcaataatgc agtctctgac gccggagaaa attgccgcca ttttaccgct gcaatttttc
    26401 ccgcaaccgg ctgattttac gggtccgtat gtgatgttgg catcgcggcg caataatcgc
    26461 gcattaagcg gtgtgatgat caacgctgat gcgggtttag caattcgcgg cattcgccac
    26521 gtagcggctg ggttggatct ttaaggaagt acgatgaaag aaaaaacgat cattattgtc
    26581 gggggcgggc aagcggcggc aatggctgcg gcctcgctac gccagcaagg gttcaccggt
    26641 gagctgcatc tgttttccga tgagcaacat cttccttatg aacgccctcc gctctcgaaa
    26701 tccatgttgc tggaagattc cccacagttg cagtctgtgt tacccgctca ctggtggcag
    26761 gaaaacaatg ttcatctgca ttccggtgta accatcaaaa cattgggccg cgacacacga
    26821 gagttagtgt tagccaacgg cgaaagctgg cactgggatc agctttttat agcaaccggc
    26881 gcggcagctc gaccgctgcc gttgcttgat gcactgggag aacgctgctt taccctgcgc
    26941 catgccggtg atgccgccag actgcgagaa gttctgcagc ccgaacggtc agtcgtgatt
    27001 gtcggtgccg gaactattgg tctggaactg gctgccagcg ccacgcagcg cagatgtaag
    27061 gtgacagtga ttgaactggc ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa
    27121 cgctatcttt tacagcgcca tcagcaggct ggtgtgcgca ttctgctcaa taatgccatt
    27181 gaacatgtgg tcgatggtga aaaagtagaa ctgacgctgc aaagtggcga gacgcttcag
    27241 gccgatgtgg tgatttacgg tattggtatc agcgccaacg accaactggc tcgcgaggcc
    27301 aaccttgata ctaccaatgg cattgtcatt gatgaggctt gccgcacctg cgatcccgcg
    27361 atctttgccg gtggcgatgt ggcaatcact cgtcttgata atggtgcact acaccgctgc
    27421 gaaagctggg aaaacgccaa taaccatgcg caaattgccg ctgccgcaat gttagggcta
    27481 ccgcttccgc tactgccgcc gccgtggttc tggagtgatc agtacagtga taacttacag
    27541 tttattggcg atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga aacccagaaa
    27601 gcgatttggt ttaatctgca aaacggcgtg cttatcggtg cggtaacgct gaatcaggga
    27661 cgtgagattc gctcaatccg caaatggatc cagagcggca aaacgtttga tgcgaaacag
    27721 ctgacagatg agaacatcgc gcttaaatca ctgtaaccag gataattagc gaatatctca
    27781 acgcctgggg cgtggcgagg taccagagtg tgtattacgt ttaaatcaca ttatcttgca
    27841 aagggattgg ttatgaactc actacgttat tttgattttg gtgctgcccg ccccgttttg
    27901 ttattaattg cccgtatcgc cgtggtccta attttcatta tttttggttt tcccaaaatg
    27961 atgggctttg acggtacggt ccaatatatg gcctcgctgg gcgcaccgat gccgatgctg
    28021 gcagcgatta ttgcggtagt tatggaagtg cccgccgcga tattaatcgt gcttggcttt
    28081 ttcacccgtc cgctggcggt actgtttatt ttctacacgc tgggtacggc ggtgattggt
    28141 caccattact gggatatgac cggcgatgcc gtcgggccaa atatgattaa tttctggaag
    28201 aatgtcagta tcgctggcgc gttcttgcta ttggcaatta ccgggccggg ggcaatttct
    28261 ctcgatcgcc gttaggaaag atgccggatg cggcgtgaac gccttatccg gcattttata
    28321 aattacagcc actctacccg caacgacatc tctgaggttg ttgattcccc cggcgcaagc
    28381 gcaatgaggt cacccccttc cgggcgatga tggtcatccg gcgcatggct catcggctcc
    28441 agacagaaga aatcaaacgc atatccttta tcaaacgccg ggtcggaaac aaagatgaaa
    28501 taacacggtg caggtggcgt cgtttctatg atgatggcat aaccctcctg cggctgctcg
    28561 atgcgggcct gaccattcca tccggcaaaa ccattgttca cccactggcg cggcaacggc
    28621 gctagctggt taaaatccag ttcttgcggt agctgctcgc aaaactcacc cgccagccac
    28681 tgctcccgct ccagccagta accgctcgcc tgtgcctgaa tccgcgtttg cggcgacaac
    28741 ggaaaataag gatgccagcc ggtaccaaat ggcagcgtct ctgccccttg attggtgaca
    28801 gagagcgtca ccgtcagcgt atccgccgtt aaatgaaacg cctgacttac ccgatagtga
    28861 tagacaccgc tgcgatgttc atacaccaga cacaaactat catcgctgtg cgagacacat
    28921 tgccactcgc ccagccagcc atcgccgtgt agatagtgcg catcccactc aacattcggt
    28981 tgcagttgat actcacgccc ctgccagaca aaacggttgc cgctcacccg gtttgcaaat
    29041 ggcaccagcg gaaaacaaga ggcatcggtt gccacaccgc tttttttacc aggacgtaag
    29101 agcggcgtcg tatcgcgcca gaagccttcg attacgccgc cctggtcaga aacgcccagc
    29161 tttagcgacc cgtgggataa ggtatagatg gtcatccgct aactccttaa tccgggaagt
    29221 taatcacaac tttcccgcat ttgccgctcg ccatcagcgc ataggcatca cctgcctgtt
    29281 ccagcgagaa gcgatgggta atggcgttac gcggccacag cttccagtca gtcaaatcgt
    29341 gggcgcattt ttccatatgg aacagactgg tcacccagga gccaataatc cgccgttgat
    29401 ggtgcatcag atcggcgctg acctcgaatt ccacttttcc ggtttcacca atgtaaacca
    29461 cccgccccca gtcagcggtg gattgcagtg ccagtaagcg acctgcggca ttaccggaac
    29521 aatcaagcgc aacatccgcg ccgccgtgag tgagttcggc gattatctgc ggcagacctt
    29581 cagtggtcgc taaatagccg tgatccatca ctcctaactg ttttgccatc gccagacgtt
    29641 ccggcagcat atcaacgccg ataatccgtt ttgcaccgcg acctttcgcc agcatcatcc
    29701 ccatcatgcc gactggcccc agaccgacca ccagcacgtt atcactgccg gaaacttcgc
    29761 cgcgcaaaat cccttcatag gccgtgccaa cgccgcaact gataaacgca ccatcttcgt
    29821 agctcagcgc atccggcagg aggatcagat ctttttcttc cgccagcaag tattcggcat
    29881 gaccgccgtc acgctgccag ccgtaagccg cttttccttc gccagtacaa gaaataggaa
    29941 aaccgcgacg gcagttcggg caaaaaccac agccagaaat gtgatacacc agcacgcggt
    30001 cgccctcttt aaaatggcgg cagccttgcc ccatcgccac aatctgcccg cacggttcat
    30061 gaccgttgat aaatccctag tacaacggtt tatcggatgc cgccgccgta gcgcggtgct
    30121 ggtggtagat atagtggaca tcgcttccgc aaattccgga ggatttcatt ttgatcagta
    30181 cctggttaat ccccggcgtc ggcactgcaa cttcccgcag atcgacggtc gaatttcctg
    30241 gtaaataagc tgccagcatc gttttcataa atccctcatt aacaatacga ttaattttca
    30301 tccctgccgc acccgcgcca ggggcgttaa ggtttagcgt ttcgctttac tgctacgctg
    30361 ggtcagcaag atattcgcca gcaccgccac cacgatgatg acgccgcgca ccacctgctg
    30421 gaaaaaggag ttaatcccga gcagcaccag accgttaccg attagcgtaa tcaccagtac
    30481 gccaagcagc gtaccgaaca acgagccgcg accaccggaa agcgccgtac cgccgaccac
    30541 gaccgcggcg atgacgtcaa actccagacc gtttgcggca cctgcgttac cggaaccgag
    30601 gcgcgccgcc aacaaaatgc cggtcaccgc cgctaataat cccgaaaggg taaagataag
    30661 aatgcgtacc cgacgaacgt taataccgca caactgcgcc gccgtggcat taccgccaac
    30721 ggcaaaaacc gagcgcccga aggcggtttt gcggctaatg aacacaaaca gcgcaaacag
    30781 caccatcatg atcagcgcgg ataccggcac accgagaaat tgtccgccca gccagtccag
    30841 cacctcgttt tcgtcaatcg gcactggcag agcgttggtc ataaacaagc ccattccgcg
    30901 cagggcgctc cacagcccca gcgtggccac gaaacttggc acgttaaaca cgccacgcag
    30961 caccccggcg agcgttccca tcagcgcgcc taacagcaac accagcagac aagcaatcgc
    31021 caacggaact tcaaattgca gcaaaaatgc caggcatacc gagacaaaag ccaccatcgg
    31081 cccaacgctg acatcaattt caccggagat aataatcagc gtcatcgccc aggcggcaat
    31141 cccaatggtg gcggcatcgc gcagcacgtt catctggtta ttcaatgaga taaagccagg
    31201 cgcgttcagg gagaagacca gataaagaat ggcaatcacc accagcaaac cgatctcatt
    31261 aatatggtga ctgacaaatt gtttgagcga gacgctcttg ccctgcggca atggtaatga
    31321 cgaagcagac atcttcaggt tcctcggtaa aattcatcag tgcacagaca gaatggcgga
    31381 catcagctca tccacattga ccggagcgtg aaactcctgc gagaacgtgc cgtgctgtaa
    31441 taacaggatg cggtcacaca ccaacggcaa ctcctccact tcactggaga taaacaccac
    31501 gctttttcct tcggcagcca gctcacggac aatacggtaa atctgctgtt tggcttcgat
    31561 atcgacgccg cgcgttggct cgtcgagcaa caaaatctgg ctggcggcat agacccaacg
    31621 accgatcact actttttgct gattgccgcc agaaagcgtg ccgatgggcg tttcgctact
    31681 ggcggccttg accgtcatcc gctgcatcac ttcttcggtc aggcggcgga tggtggacca
    31741 ttgcagcact ccgttggcgc tgattttttg ccgattggtc agcactgtat tttcgtcaac
    31801 gcccaaccag ggaatgatcc ccgcttcttt gcggttttct ggcgtatagc caatgccgcg
    31861 tttcagcatg tcgccgtaat cggggcgcgt gattttctcg ccgttgataa caatttcgcc
    31921 ctgttcatac gtctccagcc caacaatcgc cttcagcaat tcactgcgcc ctgcccccag
    31981 cagaccagca atgccgagca cttcgccacg acgtagcgta aagctgatat cctccagctt
    32041 gggcttatgg cgtaacgcac ggacttccag cacggcctga tccacaattt cctgaggtgc
    32101 aaccggcgca atatcaacgt gatcgcgccc tagcattagc gacacaatat gatgcgtgga
    32161 agtgttttcg agcatcacat cgccagccac ctgcccgtcg cgcataacgg tggcactgga
    32221 ggcaatgcgg cgaatttctt ccatccggtg gctgacataa atcaccgcca cgcccagtgc
    32281 tgacatcttt ttcaccgctc tgatcaccag ttcaacttcc gcactcgcaa gcgaactggt
    32341 aggttcatca agaatgacca cgcgcggctc gcccttcatc acccgcgcaa tttccaccag
    32401 ctgcttatgc gccgggctta gtgttgaaac aagttgttca ggactaacgt caacgcccag
    32461 cgcctgtaag caacgttggg catcctgcgc catttgcagg taatcaatca tgccgttgcg
    32521 gcggggccac tgaccgaggc agaggttttc cgccaccgtc agcccttcca ccagacttaa
    32581 ctcctgataa accgcgcgta cccccagttc agcggcacgg cgagtcagcg tagcttcgtc
    32641 accttccagt cgcgtctcgc caatccagat ttcaccgcta tccggacgtt cactgccggt
    32701 aagcattcga atgagggtcg atttgcccgc gccgtttttg cctaacagcg cacgaacctc
    32761 gcctttattg agcgtgaagt taacgttatc caacgcaacg acgccgggat aacgcttatt
    32821 tcctgccacc acttttgcta ccgggactgc ctctgttgcc gtgaacatag ggacctctgc
    32881 gaatcagcga ttagggcaga ccatcaacgt gcgttgccag ccactgttta ccgtcttccg
    32941 ttttggtata gagatcgata ggcacctgaa tcactttttc accgtcggct tgtttattga
    33001 taaccttcaa tgtttgcgcg aaaacagcat tgcccatttt cttaccggaa atatccacta
    33061 ccgctttcag cacctgattg ttttccagct cctgagcaat ttcggttgtc atatccgaac
    33121 cgaaaacagc aatttttccg gcctgatttt gattacgtac cgcttttacc gcgccgagtg
    33181 tcgcaccgcc cgattccccc ataatggcgt tgagatccgg cgtggagata atcagttttt
    33241 caccaacgga aatcgcttta tctaaaacag tcccttcctg attagcgaca atttgcgcgc
    33301 cgggaacgcg ggattttaat acttcttcaa atcctttacg tcgctgcaca caaacttcaa
    33361 aggcttcgca attgatgacg gcaattttcg gctggtcaat tttattggca ataaaataat
    33421 cggcggcagc gttacccagt tttttaccaa attccagcgg gtcacccacc agatacgccg
    33481 agacatattt atcgaccccc ttttgattaa tacaggtgtt gtagcaaatc accggaatac
    33541 ccgcttcact ggcgcgacga acggtacggc tactgccatt ttcagacact gccgataaaa
    33601 taatagcatc gacattacgc gccacgaggg tatcaacaaa ggtactttct ttcgaaatat
    33661 caccctgggc gttagtttca attaactgta cctgtactga agaatctttt gccgcatcct
    33721 gaactccctg acgcactcca gcgtaatatc cctgagtatc aaggtatatt gcgccaatgg
    33781 tcatttcttt atccgcagct ctggcaaata gtgcgctacc taatagcgta gccattaata
    33841 atagattacg ggttgttctc atttttttag gcatagagcc tcctgtaggg tttttattaa
    33901 caacggctta ttctaattat tttgtgatga gcggcagcgc gtgccgctca aatattacag
    33961 ttaatagggt ttagtgaacg tagttaaaaa taaaaggcat cacggtggtg aataatgccg
    34021 ctttcggcca ggccggatta atcgccgtca attcagcgcg agcagattcg tattgcgcaa
    34081 tctcgcctaa tcccgcggcc gccagcatcg ccaccatcag gcatttttct ttatgctgct
    34141 gttgtaaatc gccatacagc gacaacaagt caggctgcga gacggcaaag aaatccgctt
    34201 cgatactggt tttcgccatc tcttgcgccc actgtttcat ttcactaaac agttgctgtg
    34261 cggtgtgttg ttcgcccagt aatcgcagcg ccattccttg ccagaagagg taatcaaccg
    34321 gctgatcgtt gtaatagctg tggatgttaa tggtgcgatc gccggtcgcc gccagatgta
    34381 aacagcgcgt cgcttcagtt tcatcgccct gggctttggc gcatatcgcc tgccagaacc
    34441 agatgtcgtt atcagtttgc cccggtaaac ggccttcgct taaattctcc ggataatgca
    34501 gcgcggcatg aagcagttcg ctggcctgct gcggctctct ggcatcaaga tgctgccagg
    34561 cgcgtaataa ctggttgagg ataaactgac tggtgacctt cccttccccg ccttcccacg
    34621 ggtggaattt gcgcgtggcg agaatgtccg ccgctttgtc tgcctgcccc gtgagatgcc
    34681 acaaattgag cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt
    34741 ccagacgcgc cagtcgtttc tccggtgtgg ttccacttag cttatcaagc aaatcccgtt
    34801 cgaaaagcag acgtgcatcc tgcggcgcaa gctgataagc attatcaaga taacgcgcgg
    34861 ccagctcata atcgtgttgc ttattccacg catggatcgc taacccgcgc cagccgtcgg
    34921 caaactccgg cgacatctct acgcaacgtt gccataaggc aatggcttcg ttgtagctac
    34981 gtttgttgta gtagaagcag gccagtaaat ggcgagcaaa ccagcactct tcaatactct
    35041 ccagcgccgc cacttcttcc agcgtattcg ggaaacggac aaactgcggg aagacatcaa
    35101 tggcttttgc gaccagttcg ccacgttcgg ctttcggcag caagctggct tgcaggtaaa
    35161 gcggcagcgt gcgctggcag tccagtgcat tcagcatctc tgccgcgagg gtgggcattc
    35221 cccagttaat cagttgcccg gcggtcatca gggcgttaac gtcgcgtccc tgacacagac
    35281 cgcgccactg cgcgagagct gattcgctac gaccatcgaa ccagttcagg caccacagag
    35341 tggcgttcag cggataatcg cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac
    35401 gaccacttaa caccagcagc agattatgca ggcaaagcac ttcctgattg attgggcagg
    35461 cgcgaagact ttgttggcaa aaatccagac ccgcgtcgaa gttaccatta cgcgccgcca
    35521 gtcgtgccag accataatag cctccggctt tactgttgcc gctccagacc gcacgccaga
    35581 aatcctcttc ggcttgttga tattgtccct gacgttcgta agcactggcg cgaatcaaac
    35641 tcgcctgtcc gcactgcgga tttttgttca gcgcatgtgc gcgtttcaga gcctgagtgg
    35701 catacgccac cgcttgcggg aaatctgcgc ggttatactc cagcatcgcc agcgccagat
    35761 tacagcggta atccagcgga tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac
    35821 ggctggcgtg atgatattgc tccagatgct gaccgataaa ccaggcttca tctgtactgg
    35881 taatgtcttg tgctgacagt ggcgctttgg cgacgtccgg caacggcaac tcttgcggct
    35941 gatgttcctg ataactcagt acaatattgc cgtcggcatc agagagctca atggtcagtc
    36001 tttcaggatt gataccgtgt aacacgccct ggatggcggt cgctggtgtc agggccacgg
    36061 catcatcgag caacgcgtcg catttgccga tttcgcggat cgccaggcgg tatccgttca
    36121 acggagagat ggcatacagc ccccactcaa tcccccgctc actacgctgg agtttgatta
    36181 ccgcatcgcg ggaggcgttt tgcaccatgc ccagagaatg gtaaggcagg aaaaactgct
    36241 caaaacgctt ctcttcgtaa gcatcaagcc gggtaaaatc aggctggtta tcggcaaaaa
    36301 taccggtcat cagttcgata tacgggccgt tattatcagt taggctctta tcccacgcct
    36361 ggccaaattc actgtgtccc cagctccact gctttttacc tggcgcaata tggtggttgg
    36421 caacgtgtag caaaccgcca tcttcatcgt gacaccacgc gccaacaaaa tcgtactgtg
    36481 atttttcagc catatatgag gttggaacgg gcacattttt atagcgagaa atgtccactc
    36541 cagcggagta gtccactttg tagtaagtgc cggtggcgat agggaaagcg gagacggccc
    36601 gtttgccgtg atcaaacacc gccgttacat ccggcggaaa gacgctctga tgcccttcac
    36661 cccctttcac tgccgggttg gcccaccaca agaaatgacg cggcgtggcg ttgccgttat
    36721 agacgcggct ggcgatttcc agcgccgccc ggtcagggcg cagggtgaaa cctgtcatca
    36781 cctgtaaacc atgcatcggc tccgtttcgc ctacccacac cgtctgtgcg ccgtcttcat
    36841 gggcttcgag ggtgaaatca acgggcataa aggtggtcgg gcgatggtgt tgcggccagt
    36901 taaactcaat cccgccggag atccacggtc ccagcagccc caccagcgca ggtttaatga
    36961 cttcattgtg atagacaaaa tcgcgctgtt tcactttatc ccatgcgcga tgcacccgac
    37021 cgcccaattc cggcaggatc atcactttga tgtagtcgtt ttccagccac accgcctgcc
    37081 aggatttcag ggttttctgc tcgctcagcg tatcggtcac gccgtaggga taaaccgcgc
    37141 cggacgatcc ctgataaacg cgattttcca ggaacatggg atgtatatcc tgcggcccgg
    37201 tttcataggt cgggatctca acgcgctctt gccacacttt tactggagtc atggtgccct
    37261 caatattaac aagacatact gaattaaaag atttgtggca gtgtattgaa caatctggca
    37321 atgttttcgc ggaataatca cgcaattaac taaacaaggt ttagtgaaga tgagagcctg
    37381 cattaacaat caacagattc gccaccataa caaatgcgtg attctggaac tgctgtaccg
    37441 gcaaaagcgc gccaataaat caacgctggc ccggctggcg caaatttcga ttccggcagt
    37501 cagtaatatt ttgcaggaac tggaaagcga aaaacgggtg gtgaatatcg acgatgaaag
    37561 ccagacgcgc gggcatagta gcggtacatg gctgattgcg ccggaaggtg actggacgct
    37621 gtgtctgaac gtgacgccca ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc
    37681 gaaaggtgaa tttgagtatt tacagattga tgcaccgacg ccgcaggcgc tgctgtccga
    37741 aatcgaaaaa tgctggcatc gccaccgtaa attgtggccg gaccgcacca tcaatctggc
    37801 gctggcaatc cacggtcagg ttgatccagt gactggagtg tcgcaaacca tgccgcaagc
    37861 gccgtggaca acgccggttg aggtgaagta tctgctggaa gagaagctgg gcattcgggt
    37921 gatggtcgat aatgactgcg tgatgctggc gctggcggaa aaatggcaaa ataattcgca
    37981 ggaacgggat ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa
    38041 cgagcaaatt tatcgcggca gtttgtatgg tagcggacag attggtcaca ccatcgttaa
    38101 tccggatggc gtcgtctgcg actgtggacg ttacggctgc ctggaaaccg tcgcctcgtt
    38161 aagtgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta atactcagct
    38221 tgatcctgaa aaactgacta cagcgcagtt aatcgctgcc tggcaaagtg gagaaccgtg
    38281 gatcaccagc tgggttgatc gctctgccaa tgctattggt ttgagtctgt ataacttcct
    38341 caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct ttggtgagca
    38401 ctggcttaat actattattc gccagacagg atttaacccg ttcgaccgcg acgaaggacc
    38461 gagcgtgaaa gcgacgcaaa ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg
    38521 aattggctat ttgtatgttg agacgcagtt acgacagatt tgatggcgcg ataacgtaga
    38581 aaggcttccc gaaggaagcc tttttcaaca gattaaccgt ttcgcttatg cgtaaaccgg
    38641 gtaacgtgcg cagatgtcga gaactttacc tttgatgcgc tcgataacgg cttcatcatt
    38701 gatgctgtcc agcacgtcac acatccagcc agccagttct ttcgcttcta cttctttgaa
    38761 gccgcgacgc gtaattgccg gagtacccac gcgaataccg gaggtcacaa acgggctctt
    38821 cggatcgttc ggtacgctgt ttttgttgac ggtgatgtta gcacggccca gagcggcgtc
    38881 tgcttcttta ccggtcaggt ttttatcgac cagatcaacc aggaacagat ggttatcagt
    38941 accgccggaa accactttgt agccgcgttc caggaacact tctaccatcg ctttagcgtt
    39001 tttagcgacc tgctgctggt aagttttgaa ctcaggctcc atcgcttctt tcagagcaac
    39061 cgctttaccg gcgattacgt gcatcaacgg accgccctga ccaccaggga aaacggcaga
    39121 gttcagtttt ttgtacagct cttcgctacc acctttcgcc aggatcagac cgccgcgcgg
    39181 acccgccagg gttttgtgag tggtggtagt aacaacgtga gcatgaggaa ccgggttcgg
    39241 gtagacgcca gcagcaacca ggcccgcaac gtgcgccata tcaacgaaca ggtaagcgcc
    39301 gatgctgtca gcgatttcac gcattttcgc ccagtccacc acgccggaat atgcagagaa
    39361 gccaccgata atcattttcg gtttgtgttc tttggcttgt ttttccagat cggcgtagtc
    39421 gatatgaccg gtagcatcaa taccgtaagg aacaatgttg tacagtttac cggagaagtt
    39481 aaccggagaa ccgtgagtca ggtgaccgcc atgcgccagg ttcataccca gaacggtatc
    39541 acctggttcc agcagcgcgg tgtagaccgc aaagttagcc tgggagccgg agtgcggctg
    39601 gacgttagcg tagtcagcgc cgaacagttc tttcgcacga tcgatcgcca gttgttcaac
    39661 gatatcaaca tactcgcaac caccgtagta gcgtttgccc ggataacctt cagcatattt
    39721 gttggtcagc tgagaaccct gcgcctgcat tacgcgcggg ctggtgtagt tttcggaggc
    39781 gatcagttcg atgtgctctt cctgacgtac tttttcctgc tccatagcct gccacagttc
    39841 ggcatcataa tcggcaatgt tcatttcacg ctttaacatc cgcatctcct gactcagcta
    39901 acaataaaat ttttggcctt tataggcggt cctgttggac aacggcgaac agtataaccg
    39961 aatcattgtg cgataacagg tcttgacaaa ggaatttacg caaacgatta ccttcaggct
    40021 acgcaaggct ttggagaata aagagcttgc aaccggaaac ggatttcttt tcaggtttgt
    40081 gatgcaaatt tttcacttca tcacattctt tctgaaaaac accaaagaac catttacatt
    40141 gcagggctat tttttataag atgcatttga gatacatcaa ttaagatgca aaaaaaggaa
    40201 gaccatatgc ttgacgctca aaccatcgct acagtaaaag ccaccatccc tttactggtg
    40261 gaaacggggc caaagttaac cgcccatttc tacgaccgta tgtttactca taacccagaa
    40321 ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg atcaacgtga agccctgttt
    40381 aacgctattg ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct gccagcggta
    40441 gaaaaaatcg cgcagaagca caccagtttc cagatcaaac cggaacagta caacatcgtc
    40501 ggtgaacacc tgttggcaac gctggacgaa atgttcagcc cggggcagga agtgctggac
    40561 gcgtggggta aagcctatgg tgtactggct aatgtattta tcaatcgcga ggcggaaatc
    40621 tataacgaaa acgccagcaa agccggtggc tgggaaggta ctcgcgattt ccgcattgtg
    40681 gctaaaacac ctcgcagcgc gcttatcacc agcttcgaac tggagccggt cgacggtggc
    40741 gcagtggcag aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc
    40801 ccgcatcagg aaattcgtca gtactctttg actcgcaaac cggatggcaa aggctatcgt
    40861 attgcggtga aacgcgaaga gggtgggcag gtatccaact ggttgcacaa tcacgccaat
    40921 gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat
    40981 gacacaccag tgacgttaat ctctgccggt gttggtcaaa cgccaatgct ggcaatgctc
    41041 gacacgctgg caaaagcagg ccacacagca caagtgaact ggttccatgc ggcagaaaat
    41101 ggcgatgttc acgcctttgc cgatgaagtt aaggaactgg ggcagtcact gccgcgcttt
    41161 accgcgcaca cctggtatcg tcagccgagc gaagccgatc gcgctaaagg tcagtttgat
    41221 agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag
    41281 ttctatctct gcggcccggt tggcttcatg cagtttgccg cgaaacagtt agtggatctg
    41341 ggcgtgaagc aggaaaacat tcattacgaa tgctttggcc cgcataaggt gctgtgattt
    41401 gatgttgccg gatggaaaca tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc
    41461 tcttcaccgg tacggatgcg aatgacccgt gccacgtcaa agacgaagat tttaccgtca
    41521 ccgattttgc cggtttgcgc cgtgcgaata atggtatcga cacaggtatc gacaatgtcg
    41581 tccggtacga caatctcaat tttcactttc ggcagaaaat ccaccatata ctccgcgccg
    41641 cggtacagct cggtatggcc tttctggcgg ccaaaacctt tcacctcagt gaccgtcatg
    41701 ccggtaatgc cgacttcagc cagtgcttcg cggacatcgt ccagcttgaa gggtttaata
    41761 atcgcatcaa tctttttcat gctattcctt gaaaaggtcg cctgtctttt gatctgctaa
    41821 acgtaacaca taacgccaat tcattccttg aaatcgtttg catccagctc gtgtcgggaa
    41881 agcagtttat aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg ggtgacgttg
    41941 cctttggtaa tttgcagcag cttacgcaaa tagttgagtt caaactgatt acgtgcctca
    42001 acaaaggttg gcagcgccgt attttcaccc tccagcgcct gctctactaa tgcatcgcta
    42061 atcaccggag atgaggtcag cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca
    42121 ttacccggcc agctcgcggt catcaggcgt ttcatcgcat cggtggaaaa cgcgcggaca
    42181 aacggtttat gtcgctctgc cgcctggcgc aacaggtgat ttgccagtag cggaatgtct
    42241 tctgtgcgct ccgccagtgc cggaattttc aggctgacaa cgttgaggcg gtaatagaga
    42301 tcttcgcgga attccccgcg cgccatcgct tttggcagat cacggtgagt ggcagaaata
    42361 atccgcacat tgatatcaat atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc
    42421 aacacgcgca acagtttgac ctgcaacggc gcaggcatat cgccaatctc atcgagaaat
    42481 agcgttccgc cttccgccgc ctggaataaa ccttcgcgat tgctgacagc gccagtaaac
    42541 gcgccacgcg catgaccaaa cagctccgac tccagcaatt gctcaggcaa tgcaccacag
    42601 ttaatagcaa taaatggttt gctattgcgc gggctggcgt tgtggatagc ctgggcgaaa
    42661 atctctttcc cggtgccgct ctggccatta atcaaaacgc tgacgtctga ttgcgccacc
    42721 agccgcgcct gttccagcaa acgcagcatc agcgggctgc gggtgacaat cgcctcgcgc
    42781 cagcgttcgt cggtggctgg cgcggattgt tccagcgcat catcaattgc ctgatatagc
    42841 gcgtctttat caacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt tgcagcaacg
    42901 gcatcgggaa tagaaccatg cgcggtaaga ataattaccg gcattcccgg ctgcactttc
    42961 tggatttcag caaacagctg cataccgtcc atttcatcca tccgcaggtc gctgatgact
    43021 aaatctactt tttcgcgatt cagtacccgt aatccttcag cgccactttc cgccgtgacc
    43081 acgctgtagc cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca
    43141 tcgaccaata ataaatgcgc aggtttatgg ctcatcagga gtgacctcat gggtggatgg
    43201 cgcgggcttt tcactctcgt gtggcgtatc cggcgagaaa tttccagccg gtttgcgggt
    43261 cgagagctgg cgttcaatat cagtcaggtt ttccagcttg cgggtggtga gttccagttg
    43321 ctgctgtaga acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc tggactgctg
    43381 gagtttactg tagcgttggc gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg
    43441 ccagagttga tacagtgggc gaacctgtgc cgggatctcg gtacttaacg cttcaatacg
    43501 cgcgaccagc tggcggcgct catatggcgt aattttggcg tcggcgagca aaatcccttg
    43561 tttaaaggta ttttgccagc tgccgtcgtc atattggcga gcttgctgac gcgactgcgc
    43621 aggcattaaa cgatcagcac aatccatcgc ccgcagccag taaagcggat tggtttcggt
    43681 tgatttacct tgcagcgccc agatgtcgct acattcagta gaaagatagt cagccagttg
    43741 ataaaccgga attttttctt ctgctggcgt atcaatggct ggcttattgt gattctgcac
    43801 gcaacctagc aatgccagac atggcagccc tgccagccac agccgtcggg gcaataatcg
    43861 ttgaaaaatg tgtcgcatat tcaccagact taaagcctat cccagtgggc gtaattgttg
    43921 cagacagttt ggacatggac agcgcggaga aaccggagcg tacatgtcgt acgtgaggat
    43981 ttcgagcact gcccggggcc gaactgacaa ataaaatagc ctgatgggat aggctgttag
    44041 cattatttcg tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc
    44101 tcgtcgacca gatacagttc cccttgcata cggcgaatac aatccctggc aatgcttaat
    44161 cccagaccgc tgcccttcac cgcccctttt cgctggtggc ttccctgaaa aaagggttcg
    44221 aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac atcaatataa
    44281 acccgcgcac catgtaaact gctgcgaagg caaatgttac cggattcagc cccgtagtgc
    44341 accgcattgg agtaaagatt atccagtacg ctcatcagca gcattggctc cgccaggcaa
    44401 gctgttgctt tgagatcgac gtcggtatgc atcattttag cccgtgcggg caggctatga
    44461 gcagaaacta ctgtctccac cagcggtgct aactcaacat tatctagttc caccgcactg
    44521 tccgcctgtt tacggttgta atcaagcagt tgttcgatca gtttttgcaa attgcggctg
    44581 ctgctatcaa gaatgctcac cacctctttt tgctctggcg taagcggccc gacaacctga
    44641 tcagccagta attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat
    44701 aaatgtctta aaaattgatg gcgttgggat tccagccatg acaggcgctc acttaaccaa
    44761 agaatacgtt gcccaaccga gcgtaactcg ctcggtccac tgaacgaaac gctattgccc
    44821 agagaacgcc cttcccccaa ccgattgatc atccgttcaa tatttttcac cggcccgata
    44881 atcatccgcg tgaaaagcag taccattacc agactcacca gaaatagcac cagcgattgc
    44941 caaccaaaat attgcccacg ttcggcgatt tcacgctgaa gttgctgccc acgagagaac
    45001 actactgtgc gcgtggcctg taccatttcg gtattggcac tggcaaaggc ttccagacgc
    45061 gcggcagcag cagcatcggg accactgttg ttgcactgaa gttgggccag attgttcaag
    45121 tcctgacgca atgcctggta aagtttatcg tctggcagta cgcctgcgtg ggcatcgagc
    45181 atttcgctgt aacgcttgcg ctggctttga taaaccttcg caagcgtagg gtcatccagc
    45241 acgcaatact gacggtagct acgctccatc tccagcgccg cgttggtcat tgcttcgctg
    45301 cgccgggcat cgataagcgt ggtgcggttg accagcgccg cctggtcgct aagcgcattc
    45361 aggctttgcc aggcttgcca tgccagcacc aacaggggca gcagaatcag caaaaatgcc
    45421 agcattacca gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc
    45481 tcgtcagacg cgaatagcct gatgctaacc gaggggaagt tcagatacaa caaagccggg
    45541 aattacccgg ctttgttatg gaataaggcg gtgcctaact cgacgtttcg cccgatggtt
    45601 gatatagcta cgctgatatc agaagttgga cggcaggcac cttgttgtgc gtcattcgaa
    45661 ttttatgtag cacgtcccga aggggctgac ataagtcggt gaatgagcca ctgattacta
    45721 ttatgcagta actgtgccaa taaagaaaat agtttggtaa cgtactgatt atacgttgct
    45781 ttgaggggtt tatggcttcc ccgctatttg aatgtaaatc agccactgtg tcgctaaaaa
    45841 gagacaactt aagaaaaact tattagatta caataaaatc aatcatttaa gtgtcgccag
    45901 aaagcgacac ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag ctcccgaagt
    45961 tgggagctta taatagcggt tgtatttaat gccggatgcg gcgtgaatgc ctcatccggg
    46021 ctgcaaaacc aatgagtttg acggcttacc ccaactgctt acgtgcattg cggaaaatgc
    46081 gcatccatgg gccatcctcg ccccagtttt caggatgcca ggagttgctg acagtacgga
    46141 aaacacgttc cgggtgcggc atcataatgg tgactcgacc gctttcagtc gtgaccgccg
    46201 taataccgtt cggagaaccg ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt
    46261 tatcgacata gcgcagtgcc accagccctt tgctttccag tgccgccaga tgcgccgcat
    46321 cacgcacttc cacgcgccct tcaccatgag agacagcaat cggcatttgc gagcccacca
    46381 tcccctgcaa cagcagcgac gggctttggg tcacttcaac caagctgaaa cgcgcttcaa
    46441 agcgatcgga ggtattgcgc acaaaacgtg gccacaactc actacctggg atcagttcac
    46501 gcagattaga catcatctgg caaccgttac atacccccag cgccagcgtt tgcggacggt
    46561 ggaagaaggt tgcaaactca tcgcgtacac ggtcattgaa caggatagac ttcgcccaac
    46621 cttcaccggc acccagcaca tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga
    46681 aatcctccag gcccgtgcgt ccagccagca ggtcgctcat atgcacgtcg atagcatcga
    46741 agcctgcacg gtggaaagct gccgccattt caacatgcga gttcacgccc tgctcacgca
    46801 gcacagcaac tttcggacgt gcgccagtgg caatatacgg tgctgccaca tcttcgttga
    46861 tatcaaacga cagtttcaca ttcaggcccg gatcggcgtc gtttgatttc gcctgatgct
    46921 cctgatcggc acactccggg ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc
    46981 accagacacg caacgtagtg cggctttcgc tgaatacagt ctgcccgttg gcggtaatca
    47041 caaaacggtc accggaaacc gcctgcccaa cataatggac gcaatcagca agcccatgct
    47101 gtgccagtac ggactcgacc gcttcacggt cagcggcacg aacctgaatg accgcaccca
    47161 gttcttcgtt aaacaacgcc gccaggcgat cgtcacccag agtggcgata tccgcgtcaa
    47221 tgccgcaatg accagcaaag gccatttccg ccagcgttac cagcagaccg ccatcagagc
    47281 ggtcgtgata cgccagcaac ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc
    47341 ctttcagttg cgcgacatcg cgtacatctg ccggtttgtc gccaagctga cgataaacct
    47401 gcgccagcgc cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc agcagcagtg
    47461 cgttatcttc ggtagaaagc tgcggcgtga tggtgtgacg tacatcttcc acgcgggcaa
    47521 atgcagaaat caccagcgac agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc
    47581 agcgggtttt catcgacata gagtctttac ccaccgggat cgtcaggccc agcgccggac
    47641 aaagctcttc gcccacggct ttaacggctt catacaggcc cgcatcttca ccagggtggc
    47701 ctgccgccgc catccagttg gcggaaagtt taatgcgttt gatatcgcca atctgtgttg
    47761 cggcaatatt ggttaacgct tcaccgaccg ccagacgggc agaggcggcg aaatccagca
    47821 gcgcaaccgg cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg
    47881 cggtagtgac cgcgcagtta gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg
    47941 ccaccatgcc ggttacgctg cggtcaccga tggtcaccag gaaagttttc tccgccacgg
    48001 tcggcagatg cagtacgcgt ttcaccgcgt ctgcaatggt gatcccttca cggaccagcg
    48061 catcgccctt cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca
    48121 ggacgtccag cggcagatcg atcggctgat tatcaaaatg acgatcgtgc agagaaagat
    48181 gcagttcttc ggtcgcttca ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt
    48241 cgtcaaacag cggtaattga tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt
    48301 tacaccagat ttccagcggg ctcatgcccg gttcgtcgct tagaatatcg cgcagttcaa
    48361 atttaccgcc gcgcccgccg tcgctcacca gttccggcat cgcgttagaa agaccgccag
    48421 cgccgacgtc gtggataaac aggattgggt tggcatcacc aagctgccag caacggtcga
    48481 tcacttcctg gcagcgacgc tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga
    48541 ggtcggcatc agactgacct gacgccatgg aagacgccgc tccgccgcca agaccgatgt
    48601 tcattgccgg gccgccgaga acgaccagtt tcgcaccgac gttgatctcg cctttttgta
    48661 cgtgatcggc gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg tgataaccgc
    48721 gcagctcttc gccgttgtgg ctgttcactt tttcttcata agtacggaag tagccattca
    48781 gtgccggacg accaaattcg ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga
    48841 tgtccagcgc ggtgacaatg cgctcaggct taccgaaatc ttcttcccac ggctgttcga
    48901 agccaggaat tcgcaggttg gaaacggaga aaccaaccaa acccgctttc ggcttcgcgc
    48961 cgcgcccggt ggcaccttca tcgcggattt caccgccgga accggtcgcc gcgcccggcc
    49021 acggagaaat cgccgtcggg tggttgtgag tttcgacttt catcagaata tgcgccggtt
    49081 cctgatggaa atcgtagcgg cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac
    49141 cttccattac ggcggcgtta tctttataag cagagagaac gtgatctggc gtggtttcga
    49201 aagtattttt gatcatcttg aacagcgatt tcggctgctg ttcaccgtcg ataatccagt
    49261 cggcattaaa aattttgtgg cggcagtgct cagagttcgc ctgagcgaac atatacagct
    49321 cgatgtcgtt cgggttacga ccaagctttg tgaaagcatc ctgcagatag tcaatttcat
    49381 cttccgccag agccaggcca agacgcaggt tagcgtcgat cagcgcctga cggccctgcc
    49441 ccagcaaatc aacgctggta accggagtcg gttgatggtg ggcaaacaac tgctctgcat
    49501 catctaaagc aaaaaagacc gtttccatca tgcggtcgtg cagttcagcg gtaacctgct
    49561 gccattgttc attggtcagc gtaccggctt ctatatagta agcaacgccg cgctcaaggc
    49621 ggtttacctg ttgtagcccg cagttatgag caatatcggt cgctttcgaa gaccagggag
    49681 agatggtgcc aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg tggctggcga
    49741 gtgccgggcc atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg
    49801 cattgaggtc agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg agcctggcag
    49861 cctgaaaacg tgccagcagt ttgttgattc ggaatgccga cagtgcaggc gaaccacgca
    49921 gaatttccat cataagtctc tcgtcttcta agctttcggt gtacccaagg ggggaaacgg
    49981 gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca
    50041 tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt
    50101 acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacattt tttcaagctg
    50161 ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca
    50221 tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta
    50281 aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc
    50341 atactcccct gacgtataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg
    50401 cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca
    50461 gccagctgtt tgacgacctc gataatggta acgccgacct gctggcggca ggacttgtct
    50521 ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac
    50581 aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg acggcggagc
    50641 aactcaccgt tgcaccgggt catgtggtgg ttaacgatct ccagaccctg aaagaaacaa
    50701 aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag
    50761 atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc
    50821 agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgacct
    50881 ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg
    50941 aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg
    51001 attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt
    51061 taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg
    51121 cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga
    51181 tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac
    51241 agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg
    51301 tgccggagaa cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata
    51361 tgcaggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg
    51421 taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct
    51481 acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat cagattagcc
    51541 tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc
    51601 aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt
    51661 ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga
    51721 aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa
    51781 tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta
    51841 ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag
    51901 atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt
    51961 ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga
    52021 tcagacgata attttgcatc accagaccgc cctgcctcag ggccatgatt tctgcatgtg
    52081 cggtgggatc atgtcgacca atcggacggt tccagccttc gccaataaca cgattgttat
    52141 gcactaatac cgcgccgacc ggtacttccc gctcatccca ggcacgcttc gccagcgtca
    52201 ttgcatgacg catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg
    52261 ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga
    52321 gttcaccgcg cggggttact cgccagcgat gctggcaaaa gcaaagcagc gggttgtcct
    52381 gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg cgctccagtt
    52441 gcgcgacctt ttcatgcccg aggcaacgca tcgtcaatac ccaaccacca tagccacgct
    52501 gaatttggct ggcgataaga ttcacccgcg gcagccaggg tgtatcgaaa tagaccgctt
    52561 caaccagcgg ctgtggagaa ccggtaatca accagatatc agcatcggaa cttaacaggt
    52621 aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcacga aaccagcgta
    52681 cgaaatcggc ctgcaacgcc tgtaaacgtg cttcgctgtg accaaaggtg catccccaca
    52741 gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca atggctataa
    52801 tcggcaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt aaatagcgca
    52861 gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac
    52921 gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc
    52981 tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc
    53041 taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg
    53101 tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac
    53161 ttcgtatccg tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc
    53221 tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg
    53281 gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg
    53341 cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tcagtgccca
    53401 ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag
    53461 aaaatactgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat
    53521 gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc
    53581 tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc
    53641 gcgatatgca tgcgctactc gctaccgtac aggcgtcgtc ccctgacgat ctgttattag
    53701 ccatttctta caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag
    53761 tgggcggaaa agtgctggcg attaccggct ttactcccaa tgccctgcaa cagcgtgctt
    53821 ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt
    53881 gtcacgcgca gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg
    53941 aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa
    54001 agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgctg
    54061 ttaatcacta aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg
    54121 atttcaatgg gagatcatat ctatgagatt aacagcgata agtgtaccga atgcgtaggg
    54181 cactacgaga caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat
    54241 ccgcaacatg tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg
    54301 gataaaattt aatattctac
//
LOCUS       DAZGXS010000016        53816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGXS010000016 DAZGXS010000000
VERSION     DAZGXS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..98
                     /locus_tag="ACHKG5_001099"
     CDS             <1..98
                     /locus_tag="ACHKG5_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867245.1"
                     /translation="SGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            98..3206
                     /locus_tag="ACHKG5_001100"
                     /pseudo
     CDS             98..3206
                     /locus_tag="ACHKG5_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            3210..4583
                     /locus_tag="ACHKG5_001101"
     CDS             3210..4583
                     /locus_tag="ACHKG5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP0867246.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            4592..5755
                     /locus_tag="ACHKG5_001102"
     CDS             4592..5755
                     /locus_tag="ACHKG5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP0867247.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(5807..6613)
                     /gene="sapF"
                     /locus_tag="ACHKG5_001103"
     CDS             complement(5807..6613)
                     /gene="sapF"
                     /locus_tag="ACHKG5_001103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0867248.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(6615..7607)
                     /gene="sapD"
                     /locus_tag="ACHKG5_001104"
     CDS             complement(6615..7607)
                     /gene="sapD"
                     /locus_tag="ACHKG5_001104"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0867249.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(7607..8497)
                     /gene="sapC"
                     /locus_tag="ACHKG5_001105"
     CDS             complement(7607..8497)
                     /gene="sapC"
                     /locus_tag="ACHKG5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0867250.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(8484..9449)
                     /gene="sapB"
                     /locus_tag="ACHKG5_001106"
     CDS             complement(8484..9449)
                     /gene="sapB"
                     /locus_tag="ACHKG5_001106"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0867251.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(9446..11089)
                     /gene="sapA"
                     /locus_tag="ACHKG5_001107"
     CDS             complement(9446..11089)
                     /gene="sapA"
                     /locus_tag="ACHKG5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0867252.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(11402..11647)
                     /gene="ymjA"
                     /locus_tag="ACHKG5_001108"
     CDS             complement(11402..11647)
                     /gene="ymjA"
                     /locus_tag="ACHKG5_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP0867253.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(11781..13166)
                     /gene="puuP"
                     /locus_tag="ACHKG5_001109"
     CDS             complement(11781..13166)
                     /gene="puuP"
                     /locus_tag="ACHKG5_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0867254.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(13156..13320)
                     /locus_tag="ACHKG5_001110"
     CDS             complement(13156..13320)
                     /locus_tag="ACHKG5_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867255.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(13469..14887)
                     /gene="puuA"
                     /locus_tag="ACHKG5_001111"
     CDS             complement(13469..14887)
                     /gene="puuA"
                     /locus_tag="ACHKG5_001111"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP0867256.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            15099..15863
                     /gene="puuD"
                     /locus_tag="ACHKG5_001112"
     CDS             15099..15863
                     /gene="puuD"
                     /locus_tag="ACHKG5_001112"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0867257.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            15890..16447
                     /gene="puuR"
                     /locus_tag="ACHKG5_001113"
     CDS             15890..16447
                     /gene="puuR"
                     /locus_tag="ACHKG5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0867258.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            16597..18084
                     /gene="puuC"
                     /locus_tag="ACHKG5_001114"
     CDS             16597..18084
                     /gene="puuC"
                     /locus_tag="ACHKG5_001114"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0867259.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            18086..19366
                     /gene="puuB"
                     /locus_tag="ACHKG5_001115"
     CDS             18086..19366
                     /gene="puuB"
                     /locus_tag="ACHKG5_001115"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0867260.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            19404..20669
                     /gene="puuE"
                     /locus_tag="ACHKG5_001116"
     CDS             19404..20669
                     /gene="puuE"
                     /locus_tag="ACHKG5_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0867261.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(20800..21777)
                     /gene="pspF"
                     /locus_tag="ACHKG5_001117"
     CDS             complement(20800..21777)
                     /gene="pspF"
                     /locus_tag="ACHKG5_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0867262.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            21944..22612
                     /gene="pspA"
                     /locus_tag="ACHKG5_001118"
     CDS             21944..22612
                     /gene="pspA"
                     /locus_tag="ACHKG5_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0867263.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            22666..22890
                     /gene="pspB"
                     /locus_tag="ACHKG5_001119"
     CDS             22666..22890
                     /gene="pspB"
                     /locus_tag="ACHKG5_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0867264.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            22890..23249
                     /gene="pspC"
                     /locus_tag="ACHKG5_001120"
     CDS             22890..23249
                     /gene="pspC"
                     /locus_tag="ACHKG5_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0867265.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            23258..23479
                     /gene="pspD"
                     /locus_tag="ACHKG5_001121"
     CDS             23258..23479
                     /gene="pspD"
                     /locus_tag="ACHKG5_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0867266.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            23554..23868
                     /gene="pspE"
                     /locus_tag="ACHKG5_001122"
     CDS             23554..23868
                     /gene="pspE"
                     /locus_tag="ACHKG5_001122"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0867267.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            24078..25756
                     /gene="ycjM"
                     /locus_tag="ACHKG5_001123"
                     /pseudo
     CDS             24078..25756
                     /gene="ycjM"
                     /locus_tag="ACHKG5_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            25770..27062
                     /gene="ycjN"
                     /locus_tag="ACHKG5_001124"
                     /pseudo
     CDS             25770..27062
                     /gene="ycjN"
                     /locus_tag="ACHKG5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            27083..27964
                     /gene="ycjO"
                     /locus_tag="ACHKG5_001125"
     CDS             27083..27964
                     /gene="ycjO"
                     /locus_tag="ACHKG5_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0867268.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            27951..28793
                     /gene="ycjP"
                     /locus_tag="ACHKG5_001126"
     CDS             27951..28793
                     /gene="ycjP"
                     /locus_tag="ACHKG5_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0867269.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            28824..29876
                     /gene="ycjQ"
                     /locus_tag="ACHKG5_001127"
     CDS             28824..29876
                     /gene="ycjQ"
                     /locus_tag="ACHKG5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0867270.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            29894..30682
                     /gene="ycjR"
                     /locus_tag="ACHKG5_001128"
     CDS             29894..30682
                     /gene="ycjR"
                     /locus_tag="ACHKG5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0867271.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            30692..31747
                     /gene="ycjS"
                     /locus_tag="ACHKG5_001129"
     CDS             30692..31747
                     /gene="ycjS"
                     /locus_tag="ACHKG5_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0867272.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            31744..34011
                     /gene="ycjT"
                     /locus_tag="ACHKG5_001130"
     CDS             31744..34011
                     /gene="ycjT"
                     /locus_tag="ACHKG5_001130"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0867273.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            34008..34667
                     /gene="pgmB"
                     /locus_tag="ACHKG5_001131"
     CDS             34008..34667
                     /gene="pgmB"
                     /locus_tag="ACHKG5_001131"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0867274.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            34681..35763
                     /locus_tag="ACHKG5_001132"
     CDS             34681..35763
                     /locus_tag="ACHKG5_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0867275.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            35808..36713
                     /gene="ompG"
                     /locus_tag="ACHKG5_001133"
     CDS             35808..36713
                     /gene="ompG"
                     /locus_tag="ACHKG5_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0867276.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(36824..37822)
                     /gene="ycjW"
                     /locus_tag="ACHKG5_001134"
     CDS             complement(36824..37822)
                     /gene="ycjW"
                     /locus_tag="ACHKG5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0867277.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            37977..39374
                     /gene="ycjX"
                     /locus_tag="ACHKG5_001135"
     CDS             37977..39374
                     /gene="ycjX"
                     /locus_tag="ACHKG5_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0867278.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            39371..40432
                     /gene="ycjF"
                     /locus_tag="ACHKG5_001136"
     CDS             39371..40432
                     /gene="ycjF"
                     /locus_tag="ACHKG5_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0867279.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            40580..42121
                     /gene="tyrR"
                     /locus_tag="ACHKG5_001137"
     CDS             40580..42121
                     /gene="tyrR"
                     /locus_tag="ACHKG5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0867280.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(42165..42671)
                     /gene="tpx"
                     /locus_tag="ACHKG5_001138"
     CDS             complement(42165..42671)
                     /gene="tpx"
                     /locus_tag="ACHKG5_001138"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0867281.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            42790..43755
                     /gene="ycjG"
                     /locus_tag="ACHKG5_001139"
     CDS             42790..43755
                     /gene="ycjG"
                     /locus_tag="ACHKG5_001139"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0867282.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(43730..44458)
                     /gene="mpaA"
                     /locus_tag="ACHKG5_001140"
     CDS             complement(43730..44458)
                     /gene="mpaA"
                     /locus_tag="ACHKG5_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0867283.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(44749..45387)
                     /locus_tag="ACHKG5_001141"
     CDS             complement(44749..45387)
                     /locus_tag="ACHKG5_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0867284.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(45408..46339)
                     /locus_tag="ACHKG5_001142"
                     /pseudo
     CDS             complement(45408..46339)
                     /locus_tag="ACHKG5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(46339..47259)
                     /locus_tag="ACHKG5_001143"
     CDS             complement(46339..47259)
                     /locus_tag="ACHKG5_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0867285.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIA
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            47397..48296
                     /gene="pgrR"
                     /locus_tag="ACHKG5_001144"
     CDS             47397..48296
                     /gene="pgrR"
                     /locus_tag="ACHKG5_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0867286.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            48632..50245
                     /gene="mppA"
                     /locus_tag="ACHKG5_001145"
     CDS             48632..50245
                     /gene="mppA"
                     /locus_tag="ACHKG5_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0867287.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(50296..51327)
                     /gene="ynaI"
                     /locus_tag="ACHKG5_001146"
     CDS             complement(50296..51327)
                     /gene="ynaI"
                     /locus_tag="ACHKG5_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0867288.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            51571..51828
                     /gene="ynaJ"
                     /locus_tag="ACHKG5_001147"
     CDS             51571..51828
                     /gene="ynaJ"
                     /locus_tag="ACHKG5_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0867289.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(51878..52828)
                     /gene="uspE"
                     /locus_tag="ACHKG5_001148"
     CDS             complement(51878..52828)
                     /gene="uspE"
                     /locus_tag="ACHKG5_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0867290.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(52980..53732)
                     /gene="fnr"
                     /locus_tag="ACHKG5_001149"
     CDS             complement(52980..53732)
                     /gene="fnr"
                     /locus_tag="ACHKG5_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0867291.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT        13216 a        13280 c        14263 g        13057 t
ORIGIN      
        1 acagcggcga ccgactgatt gttgaaggtt ctgccaaagt cacttcaggg cagacggtca
       61 aggctgttga agttcaggct aatggaggca acgcctgatg ttttcgcgct tttttgttcg
      121 ccgcccggtc tttgcctggg ttatcgccat tttgattatg ttggcgggga ttctggctat
      181 tcgcacattg cccgtcgcgc aatatcctga cgttgcgcca ccgaccatta aaatttcagc
      241 cacttatact ggtgcttctg ccgaaacgct ggagaacagc gtgactcagg tcatcgagca
      301 acaactcact gggcttgata atttactcta tttcagctca accagcagct ctgatggttc
      361 ggtcagtatt aatgtgacct ttgaacaagg taccgatcca gatactgcac aggttcaggt
      421 gcagaataaa attcagcagg cggagtcgcg cctacccagc gaagtgcagc aaacgggtgt
      481 tacggtggag aaatcacaaa gcaacttttt gctgattgct gccgtgtatg acaccactga
      541 caaagcctcc agttcggata tcgccgactg gttggttagt aacgttcagg acccgctggc
      601 gcgtgttgaa ggtgtgggga gtctgcaagt ctttggcgcg gaatacgcta tgcgcatctg
      661 gcttgacccg gccaaactgg cgtcttactc gctaatgcct tcagacgtgc aaagtgctat
      721 tgaagcgcaa aacgtgcagg ttactgccgg gaaaataggg gcattgcctt caccgaatac
      781 tcagcaactg accgcaacgg ttcgtgcgca gtctcgcttg cagacggtgg atcagttcaa
      841 aaatattatc gtgaaaagcc agtcagacag cgcagttgtt cgtataaaag atgtggctcg
      901 cgttgagatg ggcagtgaag attataccgc tatcggcaaa cttaacggtc acccgtctgc
      961 cggggttgct gtaatgcttt cgcccggtgc gaatgcgctg aatacggcga cgctggtcaa
     1021 ggataagatt gccgaattcc agcggaacat gccgcaggga tacgacatcg cttacccgaa
     1081 agacagcact gaatttatta aaatctctgt agaggacgta attcagacgc tgtttgaagc
     1141 catcgtattg gtggtttgcg tgatgtattt attcctgcaa aacctgcgtg ccacactgat
     1201 tccggcgtta gctgtccccg tcgttttgct gggcacattc ggcgttcttg cgttgtttgg
     1261 ctattcgatt aataccctga cgttgtttgc tatggtgctg gcgatcggct tgctggtgga
     1321 cgatgccatt gtggtagtgg agaacgtcga acgcattatg cgtgacaaag ggttgcccgc
     1381 gcgtgaagcc acggaaaaat caatgggcga gatttctggc gcactggttg ccattgcgct
     1441 ggtgttgtca gccgtattcc tgcctatggc cttctttggg ggatctacgg gggtaattta
     1501 tcgtcagttt tcgataacca ttatctccgc aatgctgctt tccgtggtgg tggcattgac
     1561 cttgactccc gccctgtgcg gttccgtcct ccagcatgtt ccgccacata aaaaaggctt
     1621 tttcggcgca tttgaccgct tttaccgccg tactgaagat aaatatcagc gaggcgtaat
     1681 ttatgtcctg cgccgtgctg ctcgaacgat ggggctttat ctcgtactgg gtggtggaat
     1741 ggccctgatg atgtggaaac tgccgggcag tttcttaccc actgaagacc agggcgaaat
     1801 catggtgcag tacacgctgc cggccggggc gaccgctgcc cgtacagcag aagtgaatcg
     1861 ccagattgtt gactggttcc tgattaacga gaaagcaaat accgatgtca tttttaccgt
     1921 tgatggtttc agttttagcg gcagcggaca gaacaccggg atggcgtttg tttcgttgaa
     1981 aaactggtct caacgtaaag gggcagaaaa caccgcccag gctatcgccc tacgggcaac
     2041 caaagagctg ggcacaattc gtgatgccac ggtatttgcg atgacgccgc cagccgttga
     2101 tgggctgggg caaagcaatg gttttacgtt tgaattgtta gctaacggtg gaaccgatcg
     2161 cgaaacacta ctgcaaatgc gtaatcagtt gatagaaaaa gcgaatcaaa gtccggagtt
     2221 acattctgtt cgtgccaatg atttgccaca aatgccgcaa ttgcaggtag atattgatag
     2281 taataaagcg gtgtcattag ggttgagttt gaatgatgtc accgacaccc tgtccagtgc
     2341 gtggggcggt acttatgtga atgactttat tgaccgtggt cgtgtgaaaa aagtctacat
     2401 tcagggcgac agtgaatttc gttccgcgcc gtcagactta ggtaaatggt ttgtgcgcgg
     2461 tagtgataac gccatgacac cattctctgc ttttgcgacc acccgctggc tgtatggacc
     2521 ggaaagactg gtgcgctaca acggttctgc agcctatgaa attcaggggg aaaatgcgac
     2581 tggctttagt tccggcgatg caatgacaaa aatggaggaa ctggcaaaca gtcttcctgc
     2641 gggaacaacc tgggcctgga gtggtttgtc attgcaggag aaactggcca gcggtcaggc
     2701 attgagtctg tatgcagttt ctattctggt ggttttcctc tgccttgcag cactgtacga
     2761 aagctggtca gtcccgttct cggtcattct ggtgatccct ctggggcttc ttggcgcggc
     2821 gctggcggcc tggatgcgtg atttaaacaa cgatgtttac ttccaggtgg cgctattaac
     2881 cactatcggc ttgtcgtcga aaaacgccat tctgattgtg gaatttgctg aagcggcggt
     2941 tgcagagggt tattccttga gtcgtgcggc attacgcgcg gcgcagactc gtttacgccc
     3001 aatcatcatg acctcgctgg cgtttattgc ggggggtaat gccgctggcg atagcaaccg
     3061 gctcaggggc gaacagccgc atcgccattg gtacgggcat tattggcggt acgctgaccg
     3121 ctacgttgct ggctattttc tttgttcctc tgttttttgt actggtgaag cgtttgtttg
     3181 ccggtaaatc gcgccgtcag gagtaagtta tgttgcgtcg ttctcttatt tttctggtgc
     3241 tgttgagtgc gggatgtgtc tcgctcgatc cccactacag cacgcctgaa tccccaatcc
     3301 cggcgacctt gccgggagca cagggccagg gaaaggcaat cagccatgac tggcagcagg
     3361 tgatccacga tccccgattg cagcaggtgg tgactattgc actgaacagt aaccgcgatg
     3421 tgcaaaaagc gattgctgat attgactcag cacgggcgct ctatgggcaa actaatgcgt
     3481 cgttattccc gacggtgaat gcggccttaa gttcaacccg cagtcgttcg ctggcgaatg
     3541 gtacggaaac cactgccgag gctgacggca cggtgtccag ttttacgtta gatctgtttg
     3601 gtcgtaatca gagtttatct cgggcggcgc gagaaacctg gcttgccagt gaatttacag
     3661 cccagaacac ccgtttaacg ctgattgctg aaatcagcac cgcctggctg acactggcgg
     3721 ccgataacag caacctggcc ctggcaaaag aaacgatgac cagtgcagaa aactcattga
     3781 aaattatcca gcgccagcaa caggttggca cggcggcagc gacagatgtc agtgaggcga
     3841 tgagtgttta tcagcaagcg cgcgccagcg ttgccagcta tcagacacaa gtgatgcagg
     3901 ataaaaatgc gctgaactta ctggcaggta caacgcttga ggaaaatttg ctaccgggaa
     3961 cactggaaag cctgccggaa cagatgataa gtctggttcc tgcgggagtg tcatctgacg
     4021 ttctgctgcg tcgcccggat atccaggaag cggaacataa tttgaagagc gccaatgccg
     4081 atattggcgc ggcgcgggct aactttttcc cgacgatttc gctgactgcc agcgctggtg
     4141 ttggcagtga cgcattgtcg tcgttgttca gccacggaat gcagatctgg tcatttacgc
     4201 catcggtcac attaccgctg ttcactggcg gaagtaatct ggcgcagctt cgttacgcag
     4261 aagcacaaaa gcgtggactc attgccacct atgaaaaaac cgttcagaga gcatttaaag
     4321 atgtcgctaa cgcactggcg cggcggacca cgcttgaaga gcaactggat gcacagcgcc
     4381 agtatgttaa agctgagcaa caaacggtcg atgttggctt gcgtcgctat cagacgggtg
     4441 ttggtgatta cctgaccgtg ttgacggcgc agcgcagctt gtggagcgca cagcaggagc
     4501 tgctggcact gcaactgact gattttacaa accgaatcac gctctggcag tcgctgggcg
     4561 gcggcatgtc atcactcaag taaggaataa tatggctaga gtctctcttt catgggcatt
     4621 gattcttggt cttttagccg gtatcggccc gatgtgtacc gatctttatt tgccggcttt
     4681 gccggagatg tctgagcaac tggcggcaac cacgaccata acgcaattaa cgctgactgc
     4741 atcactgatc ggtcttggcg tcggacaact gttatttggc cctctgagcg acaaaatagg
     4801 gcgtaaacgt ccgttaatct tgtcgttgct attgtttatt gtttcttcca ttttgtgcgc
     4861 gacaacgaac aatatttact ggctggtggt ctggcgtttt attcaaggga tcgcgggggc
     4921 gggtggttcg gtgctctctc gttctattgc tcgtgacaaa tatcagggag taacgttgac
     4981 ccagtttttt gcgctgttaa tgacggtgaa tggcctggca ccggtgttgt cgccagtgct
     5041 gggcgggtac attgttagca cttttgactg gcgcacttta ttctgggtaa tggctgaaat
     5101 tagcaccgta ttgttgctgg gctgcgtgtt atttattaat gagaccttgc cagaaaataa
     5161 aaggggctca tcattgctat taaccggacg aagcgtggtg cagaaccgcc gttttatgcg
     5221 cttttgcctg attcaaagtt ttatgctggc aggcttgttt gcgtatatcg gctcttcgtc
     5281 gttcgtgttg cagaaggaat ttggctttag tccaatgcaa tttagcctgg tgtttggcct
     5341 taacggcatc ggacttatca ttgcttcatg gatcttttcg cgtctggcgc gacggattaa
     5401 tgcgatgaca ttgttgcgag gtggcctgat agcggcaatt ttgtgtgcat tgctcacggt
     5461 cttatgcgca tggacacaat tgcccattcc ggcactggtg gcattatttt tcactattgc
     5521 attttgtagc ggcatcggca ctgttggcgg ggcagaggct atgagtgcag tagggacgca
     5581 ggaatctgga acggcgtctg cgttgatggg gatgagcatg tttgtcttcg gcggtatagc
     5641 cgcgccattg tcgggatttg gcggagaaac actgttaaaa atgagtctgg caattacggt
     5701 gtgttataca ctggcattcc tggttgctct caccagaatc gacaatcaaa agtaatgaat
     5761 acctcatcct gaggggggtt cccctgtcag gatgaggtgc agaacactaa cggtccttac
     5821 gccacgcatc cgccgtcaat gcttcgccaa agtgcccggc aatcagccgt ttggtgagtt
     5881 catgcagcgg cgatgccagc acatccgcgg tgctgcctcg ctcgacaacc tcgccctgat
     5941 gcatcaccag cacctggtcg ctaatgtgct tcatcattcc gatatgctgg gtaacataaa
     6001 tatacgaaat gccctgtttt tcctgtaatt ccagcatcag attaatcaac tgcgaacgca
     6061 tcgacatatc cagtgaagcg agggcttcat cggcaataat gacttttggg cgcaatatca
     6121 gcgcgcgcgc cagacccaga cgctgttttt gcccgggggc caacatatgc ggatagtaac
     6181 tgacgtgatc cggtagcagc ccaaccatac gcatcgtttc aataatctgt ttgcgacgct
     6241 gttccggttc caggttggtg ttcaggcgca gtggaaaatc cagaatttgc gagatacgtt
     6301 gacggggatt caacgaggtc gaaggatcct gaaaaatcat gcgaatacgc tgactgcgga
     6361 aggaataatc gccaaaatgc agtggatgat cgtcaatcaa taactcgccg ctggtaggtt
     6421 ctatcattcc cgccagcatt tttgccagcg tggatttacc cgaaccattc tcgccaataa
     6481 tcgccagtgt ctggccttca cgtagcgtaa agctcaaggg ttttaccgct tctacggtct
     6541 gacgacgaaa ccaaccggtc cggtagcgga acgttttact taaattacgc acttcaagca
     6601 gcgtttcgat catctcactc tttctccatg ttcagcggga aatgacaggc atagagatga
     6661 tttttcgccc ccgtcaaacg tggcgtcaca atgcattctc gttgtgcata cgggcaacgt
     6721 ggccccagac gacaaccaat cggtaactgt tcaagcagtg ggatagcgcc gggtagcgta
     6781 ttgaggcgac ttttatgcgg catcgcgctt ccgaagtctg gtatcgcgcg gatcagcgcc
     6841 tgggtataag gatgatgtgg catcgtcact aactccttac tcggcgcggt ttccaccgtt
     6901 tgaccgcagt aaagcacgtt aattttatcc gcccattggc taagcatttg taagtcatgg
     6961 ctgataagca aaatagtggt attgctgttt tggtttagac gcgtcagcag gcgaaagatt
     7021 tgcgcctggg ttgttggctc cattgagttt gtcggttcgt cagcaatcag cagacgcggt
     7081 tgattcgcca gtgcaatggc tatcatcact ttctgacatt caccttcggt caactcatag
     7141 ggaaaactgc gcatcgcatc tttatgatct ttaattccca cgcggtgcag cagttcaatg
     7201 gcacggcgtt tgcgccagcc aaaacgctgc caccaacggc ctttataggt ccaggctggg
     7261 atgttttgca tcaactggcg acccacacgt tctgaaggat ctagacacga ctgcggttcc
     7321 tggaaaatca tcgacacgtt gtggccaacc agtttgcgcc gttcgcgtgc ggagagacgc
     7381 agcaaatcga tatcatcaaa acgcatacgg tctgcagtaa cacgccagtt atctttattc
     7441 accccacaaa tcgctttcgc aatcaaactt ttgccggaac cggattcacc aacaagaccg
     7501 cggatttcac cttcggttaa ggtcatactt acccggtcaa cggctttaac ccactcatca
     7561 ccggttttaa attcaatagt caggttacga atatcgagta atggcattat tccacccccg
     7621 caataattgc acgacggaca ccgtcgccaa ggaggttaac caacagcacg ctaatcataa
     7681 ttgccgcacc tggcagcatg acagtccacg gggcgacata aatcagttcc agcgcatcac
     7741 cgagcatcgc tccccattca ggcgagggga gttgtgcgcc aagatcgaga aagcccagcg
     7801 cggcgatatc gagaattgcc atcgacagtg cgcgggtgat ctcggtgacc agcccggcgg
     7861 tgatgttagg catcacagca aaccagagaa tattcagcgt tgatgcgcca tccagacggg
     7921 cggcgataac gtactctttt tccagttcgt catgcaccat gctgtaaatc gaacgtacca
     7981 tacgcggcag cagcgccagc cagacagcaa acatagcgtg ggacaaactc ggtccggcaa
     8041 acgctacaac gataattgcc aacagcaggg aagggatcgc cagtaaggta tccagaatat
     8101 ggttaagcac cgctgagcgg aggccgtgcg tcgccccggc gaaggtgccc agtaccaggc
     8161 cacagatcgt cgcggcaagt gtcaccacaa atgcgccacc cacggttggc gcagctccgc
     8221 tcagtaaacg gcttaaaaca tcgcgtccga ggtcgtcagt ccccaggaag aaagaaactt
     8281 cgccatagcg cgaccatgat ggcggcaata attggtaacc gagaaattgc tggtcgatgc
     8341 cgtaaggcgc aaaccagccg ccaaacatac acagtacagc cagccccgcg cagccgtaca
     8401 ggccgaccat cgcagaggcg tcactataaa atttgcgcca ggcggtacgc agcgtgcccg
     8461 gcgggcgctt ttcgctgtat acgctatcgt aaggcatacc attccttatg tttcagaggg
     8521 ttagccatgg cacccaaaat atcagaaatc acgttaacaa taatgaccag tgagccacac
     8581 accatcactc cggcggaaat ggctgcgtaa tcctgctggc ggatggcgtt aattaaccag
     8641 cgccctaaac ccggccagct aaaaaccatt tcggtgatca tcgccagcgt cagcatggtg
     8701 gaaaactgta agcccaggcg aggaataacc ggcggtaacg cgttatgcag aacgtggcga
     8761 cgcaaaatag taaagcgcga caaaccgcgg gtcgccgccg cttttacata gttctggtca
     8821 tacacttcga tagtgctgat acgcatcagg cggatcactt cagttgttgg cgcaaccgac
     8881 agggtgatca cgggcaatat catatggcgg attgcgctca tgatcatttc atcccgccat
     8941 ggtgagtccg aaagccaggc atcaatcaac gcaaaaccgg taatcggttt cacttcgtag
     9001 agcagatcga aacgccctga aacgggcaac caacctagcg tgagtgaaca aaacagtgtc
     9061 aacagaagcg ccagccagaa caccgggatt gaaaagccca gtaaggcgat ggcgttgatc
     9121 aagttatcct gccacttatg gcgcgtaatc ccggcaatca tccccacggg gatgccaaca
     9181 atcagcgcaa aaccgaaggc gaggatgcac agttccatcg tcgccgggaa gacctctttt
     9241 aactgttcgg caattggttg accgttaatg ctggaaacac caaaatccca gtggatcagg
     9301 ccgttaaacc agaacaccca ggcattccac agtgacgcgc cttgtaacgg cgcgtgtgga
     9361 gtgaaatagc tcaagctgaa gccaacgaag gtcagcagga acaaggtgac aatcaataac
     9421 aaaatgcggc gtaaggtgaa gataatcatg gttttttcac ctcatcctgt ttctcgcgat
     9481 acaccccagc aaaggaggcg ttaccaaacg ggctaagtac cagacctttg atatcgtacc
     9541 gataggcctg caaacgcaat gacgacgcca gcggcaaaat gggcaattcc tgcgccagaa
     9601 tactctgcgc ttcgtcatag gcttcaatac gcgccgccag ctgctgcgag gagagcgtct
     9661 tacgcaacac gctgtcgaat ttcggatcgc accagtgggc gaggttggtc tgtgaatgaa
     9721 ttgccgcgca gctcagtaac ggacggaaga aactgtccgg gtcgttactg tccgtcgccc
     9781 aaccggataa cgtcagatca tggctcatat ccatcaaccg cgcctcctga aagcgacctt
     9841 ctaccggcac aatcaccact tttacgccaa cctgcgccat atccgcctga atcagttcgg
     9901 cagttttcag tggactgggg ttccacgcct gcgaacgtgt gggcacccac agtttcagcg
     9961 ttaaattttc cagccccaac aacttcaact gttcgcgcga tttcgccgga ttgtattcag
    10021 taattttagc ctcgttgtca taggcccacg aggcgcgcgg taaaatagag gccgccgttt
    10081 cagccgtacc ataatagatg gattgcatca ggcgctggtt attaatcgcc agtgccagcg
    10141 catggcggac agcgggatta tttagcggcg gtttggcggt gttaaatgcc agataggcga
    10201 cgttcatccc aggacgcagc gttaaacgca agcgcgggtc gtcacgcaaa atggatagct
    10261 ggctggcagc aggccaggcc agaacgtcgc attccccggt caggagtttc gacagacgtc
    10321 cggtgccgcc ggagcctaaa tccaccacca cctgcggcat taacggttta ccgcgccaga
    10381 agtcatcatg acgttgtagg cgaataaatt gcccggcgcg gtattccgac aactgatacg
    10441 gtccagtgcc gaccggttga cggtcgagtt gctcctggcg atcttctttc tctaacttcc
    10501 gggcatattc tgccgacatg accgaagcat aatgggttgc gaggtgccac aaaaaagaag
    10561 catccggctg agccagacgg aactcaacgg tatgattatc cagtttacgg acgcttttca
    10621 cgttatcggc aaattgcagg ctgtcgaagt aggggaagtt gctgccgttg acgttatgcc
    10681 acgggttgtt gcggtcaaaa attcgctgga aggtaaacac cacatcgtcg gcattcattt
    10741 tgcgagtggg agtaaaccag gcggtttttt gaaacgggac atcgcgacgc aggtggaagc
    10801 gataggtcgc gccgttgtcg agtacttccc agctttcggc aagttctggc atcaggcgat
    10861 aggtataggg atcgacatcc agcagtcgat cataaaactg ggcggcaagg gtatcgacaa
    10921 ttaacccact gctcgctttg gatgggttaa aggtgttgac ttgcccgctg acgcaataga
    10981 caaaaccgct gtcgcggata tcagcatgcg ggggggattc aggcgcggcg attgcctgac
    11041 cactcacaag tccagcaatc accaaaagag acgataatac ctggcgcata atattaaggg
    11101 attttatgta aagaggctat cttactaata tttaatgaca tttgccatta ccgtttgtgt
    11161 tcaggggtcg taatgagggc cgtgtggttg gtcgttgaac agatggcgtc gccatctgtt
    11221 caacattcgt acccgtgatt tctctataac tataactcac agaacaactt agcgaggagc
    11281 agagcggtac gatcgtgaga aacccattaa agcacgctgc agtgcgtgtc attgttagcc
    11341 ggatgcggcg tgaacgcttt atccggacaa cgatactgac cgatcgtctg caattaaata
    11401 attactcatt accccattga ttcaaaaact ctgcgatctc atcaatgcgt acgggattaa
    11461 tccccgcttc agcagccatt tcatgctggg cttcttcgct gatctcttca ttgttcatca
    11521 aacgggtgag cagtaactgg aagtagtggg ccagcggtgt gcgttcagcg tctgcgacag
    11581 gctctgcgca ttcggtcgcg tactcatcgg caatatcaaa atattttagc gggatatcgt
    11641 ggttcattat ttgcccctgg ggttaatgcg ctaagcataa gggcggatga tagcactcgt
    11701 gatccgggtc atacatcttt ttatagttaa tggcgcggcg cattaccctc aggcaggata
    11761 atgcggcgcg catccgacta ttacgtttcg ctcactggcg ttctgtcacc ttcgtacagc
    11821 ggcactttgc gatagcggcg gatcaaatac cacaaatatg cgccgcctaa cgaagcccat
    11881 accagaccga gtgtcagtga cgttgactcg aggttaaccc acagcacacc caccgtcagc
    11941 gcgccaacca gcggcatcag caaatagtgg aagtgatcct tccagctttt attcattcct
    12001 ttacgccgcc agaaatgatt aaacaccgac agattcacga aggtaaacgc caccagagca
    12061 ccgaagttaa tcaatgctgt cgcggtgact aaatcaaaga acaatgccga catcgcgaca
    12121 attccgacca taatgacgtt cagtgccgga gtccgccatt ttgggtgcac atagccaaac
    12181 acgcgctccg gaaacacatt gtcgcgcccc atcacataca gcagacgtga cacgctggca
    12241 tgtgaggcca ggccagacgc taacgtgttc acaaacgtgg tgcagaggaa aatcgactgg
    12301 aacagcttac cgccgacgta gagtgcaatt tcaggcagtg cggcgtccgg gtctttaaag
    12361 cggctgatat cggggaagaa cagctgcata aagaacgacg ccgcgataaa gataacgcca
    12421 ccatagaccg ccgtcaggaa gatggctttc gggatcacgc gtgcggcgtc tggtgtctct
    12481 tccgaaagtg tggtcaccgc atcgaaaccg aggaacgaga aacagacaat cgtcgccccg
    12541 gtaataatcg ggatcaggtg cgcgttctcg ctgataaacg gctgaagtga ccagacggtg
    12601 ccaacgcctt ctcctttatg cagtccctga accaccagga agataaacac caccatgatg
    12661 gagatttgca ccagtacaaa cagggtattg aagttagcga ccaggttgac gctcttcaga
    12721 ttcgcggcgg ttaaaatggc gacgaaggtt accacccaca cccacggcgg cacttccggg
    12781 aagagggcgg agagatagat tttcgccaac aaaacgttga tcatcggcaa aaagagataa
    12841 tccagcagtg atgaccagcc gaccataaat ccgacgtgcg ggttaatcga cttttgcgcg
    12901 taggtatagg ccgaaccggc ctccggaaac tggcgaacca gtttgccgta gctgatagcg
    12961 gtaaacagca cgcccgccag cgccagcaaa taggacgcag ggacgtgacc gtcgctaatg
    13021 ccagatacaa tgccaaaggt atcgaacaca gtcatcggcg tgagataggc cagacccatc
    13081 atcacaacct gccacaattt cagtgatttt cgcagacggg ttttgccggg ttgcgcagca
    13141 atattcagtg gtgaattaat agccataatt gctgcctccc ctgcgtaata aggtttgcgg
    13201 tcgtgtcgac ctgaaatcaa acctgtttag caagggaagc aaagggggcg gagtccagtc
    13261 agtcgcagga ggggcgcata cgcagcctcc cgcgccatgc ggggatttaa tcatcggcat
    13321 catctcattt cctcgtcaca gtcttaatcg ttaacggaag cccgttgctg cctgcgctcc
    13381 ataacgcgac atccgggaaa tgtctggatt accagcaaat gtcaggcctg gctccgctca
    13441 ggccgatgaa acaaccccgc aaggggtact atgcgttttt caacatccac tcaatttctg
    13501 tttctgtgat gaggcgctca aactgcaaca gctcatcatt tttacaggcg tgatacacat
    13561 ggcaaaaccg ttcgcctaaa tagcggcgca ggtgatcatt ctcgataaac tcacccaagg
    13621 catcgctctg gcgaatcggg aagggtaagc cttcctgttc cagcccgtta ccttcgactt
    13681 cttcctgcaa cggcagctcg ttatcaaggc catgcaaaat accggcaaaa atcgctgcca
    13741 tcaccagata tgggttggca tcggcacccg ccacgcgata ttccacgcgg tgattatgac
    13801 gatcgccgca tggaatacgc agggcgacgg tacggttatt atggccccac gacgcctgcg
    13861 tcggtacata catccccggc tggaagcggc gatacgagtt cacgtttggt gccagcaacg
    13921 ccattgatga cggcatcagg tcaatcatcc cggcgagcat ctttttcagc agcggcgaat
    13981 cttcgccttc tgtgtcagaa agcacgttct cgccacgatt attttgcata ctgatatgga
    14041 tatgcattcc gctgcccgcg tgctcttcat acggcttcgc cataaaagtg gcgtgcatct
    14101 tatgcttttc tgccatcaga cgcaccaggc gttttagtgc cagcgcatca tcgcaggctt
    14161 ccagcacgtt atcagtatgg taaaggttga tttcaaactg acccggcgag gcctcagcga
    14221 ccgcgccatc tgccggaatc agctgtaact gcgccagttc atcaatatca ttgagcacgt
    14281 cggcgaagtg gttgaggtta tcaacggagt aaacctggct ttgtgtattg cggtcatcag
    14341 tgccgggcgc gcagggcggt tgcagatacc cttcagcgtc gcgctggcga tccagtaaat
    14401 agaactccag ctctaccgct acgaccggga acaatccgcg ctggcgcagc tgctgccaca
    14461 ggcggttaag aacgttccgc ggctcaacgt caaagggagc gccatcttca tcgaccatgg
    14521 tcaggagcat ctgaccgata aactctggat cggcggcaga aggggttaag gaaccgagaa
    14581 caggaacaca ggtacgatcc ggctcgccca tttcctgacc cagacctgct tcttctacta
    14641 cgttgcccag aatatccatt gcaaataccg aggccgggaa ataacaccct ttctcgagct
    14701 tcttcaggct tgaaacagga atacgttttc cacggaagca accattcaaa tcggtaagta
    14761 aaacatcaac atattgcgta ttcgggtagc gctccaggta gcgtttcact tcctgcgtaa
    14821 aggcgctacc ccgcctctct tctgactgct gaacaaagtt ctctacttca acgatattgg
    14881 tttccatgat tcttcgcctt tggtttgttt tccgctcgtt atcaaagcgt aaaatataat
    14941 gaccaccatt cgaatctgta tgcaaactaa atgtttgtca aatgttaaat tgagtttgca
    15001 aaaatgaaaa cccactgcta gattgaaaaa atattgaaca taaaggtcat ttaaaccgca
    15061 gtaatggcga taatttagtc cactttgtga gattgagcat ggaaaatata atgaacaatc
    15121 cggttatcgg tgtcgtaatg tgcaggaaca ggcttaaggg gcatgcgacc cagactctgc
    15181 aagaaaagta cctgaatgcc atcatccacg caggcggctt gccaattgcg ctgccacatg
    15241 cgctggcgga accgtcgtta cttgaacaac ttttgccgaa actcgatggc atttaccttc
    15301 ctggtagtcc cagcaatgtg cagccgcacc tatatggtga aaacggcgat gagcctgacg
    15361 ccgatcccgg gcgtgatctt ctgagcatgg cgttaattaa tgccgcactc gaaaggcgca
    15421 tccccatttt cgccatctgc cggggtttac aggaactggt ggtggcaacc ggtggttcgt
    15481 tgcatcgtaa gctgtgcgaa cagcctgaat tgctggaaca tcgggaagat cccgaactgc
    15541 cggtggaaca gcaatatgca ccgtcgcatg aagttcaggt tgaagagggg ggattgctgt
    15601 ctgcattgtt acctgaatgt agcaactttt gggtaaactc tctgcatgga caaggggcga
    15661 aggtcgttag cccacggttg cgtgttgaag ctcgctcgcc ggatggctta gttgaggcgg
    15721 ttagtgtcat caatcatcct tttgcgctgg gcgtacagtg gcacccggaa tggaacagta
    15781 gcgagtacgc gctttcgcgt atattgttcg agggctttat caccgcttgt cagcaccata
    15841 tcgctgaaaa acagcgactc tgaccactac agtttaagga aatgcaaata tgagtgatga
    15901 gggactggcg ccaggaaaac gcttgtcgga aatccgccag cagcaggggc tttcacaacg
    15961 tcgtgccgcc gaactctccg ggctgactca cagtgctatc agtacgatag aacaagataa
    16021 agtcagccct gccatcagta cgctgcaaaa gctgctgaag gtgtatggtc tgtcactctc
    16081 ggaattcttt tccgagccgg aaaaacctga tgagccgcag gtcgtcatta atcaggacga
    16141 tttaattgag atgggcagtc agggtgtgtc aatgaagctg gttcataacg gtaacccgaa
    16201 tcgcacgctg gcgatgatct ttgaaacgta ccagccgggc acaaccactg gggaaagaat
    16261 taagcatcag ggtgaggaaa taggcactgt actggaaggt gaaattattc tgacgattaa
    16321 tggtcaggat taccacctcg tcgcggggca aagttatgcc attaataccg gcatcccgca
    16381 cagtttcagt aatacgtcgg caggtatttg ccgaattatc agcgcccata cgcccaccac
    16441 gttttaatct ttttgttctg taagccgggt aagcgcaatg cgcccggcaa tccataaaga
    16501 atgtcgccag aaataaacgg gcatacggcc cggggatctc tgcgccctga cgttcacaaa
    16561 ctgcatatat ctgatagacg tgaaacagga gtcataatga attttcatca tctggcttac
    16621 tggcaggata aagcgttaag tctcgccatt gaaaaccgct tatttattaa cggtgaatat
    16681 actgctgcgg cggaaaatga aacctttgaa accgttgatc cggttaccca ggcaccgctg
    16741 gcgaatattg cgcgcggcaa gagcgtcgat atcgaccgtg cggtgagcgc agcacgcggc
    16801 gtatttgaac gcggcgactg gtcactctct tctccggcaa aacgtaaagc ggtgctgaat
    16861 aaactcgccg atttaataga agccaacgcc gaagagctgg cactgctgga aactctcgac
    16921 accggcaaac cgattcgtca cagtctgcgt gatgatatac ccggcgcggc gcgcgccatt
    16981 cgctggtacg ccgaagcgat cgacaaagtg tatggcgaag tggcgaccac cagtagccat
    17041 gagctggcga tgatcgtgcg tgaaccggtc ggcgtgattg ccgccatcgt gccgtggaac
    17101 ttcccgctgc tgctgacttg ctggaagctt ggcccggcgc tggcagcagg gaacagcgtt
    17161 gtcctgaaac cgtctgaaaa atcaccgctc agtgcgattc gtctcgcggg gctggcgaaa
    17221 gaagcaggct tgccggatgg tgtgttgaac gtggtgacgg gttttggtca tgaagcgggg
    17281 caggcgctgt cgcgtcataa cgatatcgac gccattgcct ttaccggttc gacccgtacc
    17341 gggaaacagc tgctgaaaga tgcaggcgac agcaacatga aacgcgtctg gctggaagcg
    17401 ggcggcaaaa gcgccaacat cgttttcgct gactgcccgg atttgcaaca ggcggcaagc
    17461 gccaccgcag caggcatctt ctacaaccag gggcaggtgt gcatcgccgg aacgcgcctg
    17521 ttgctggaag agagcatagc cgatgaattc ttagccctgt taaaacagca ggcgcaaaac
    17581 tggcagccgg gccatccact tgatcccgca accaccatgg gcaccttaat cgactgcgcc
    17641 cacgccgact cggtccatag ctttattcag gaaggcgaaa gcaaagggca actgttgttg
    17701 gatggccgta acgccgggct ggctgtcgcc atcggcccga ccatcattgt ggatgtagac
    17761 ccgaatgcgt ccttaagccg cgaagagatt ttcggtccgg tgctggtggt cacgcgtttc
    17821 acatcagaag aacaggcgct acagcttgcc aacgacagcc agtacggcct tggcgcggcg
    17881 gtatggacgc gcgacctctc ccgcgcgcac cgcatgagcc gacgcctgaa agccggttcc
    17941 gtcttcgtca ataactacaa cgacggcgat atgaccgtgc cgtttggcgg ctataagcag
    18001 agcggcaacg gtcgcgacaa atccctgcat gcccttgaaa aattcaccga actgaaaacc
    18061 atctggataa gcctggaggc ctgaaatgac agaacatacc agcagttact acgccgccag
    18121 tgcgaataaa tacgcgccat tcgacacgct gaatgagtca atttcctgcg acgtttgcgt
    18181 ggttggcggc ggctataccg ggctgtcttc cgcactgcat ctggcggaag cgggctttga
    18241 cgtggtggtt ctggaagcct cacgcatcgg ctttggcgcc agcgggcgca acggcgggca
    18301 gcttgtgaac tcctacagcc gcgacatcga cgttatcgaa aaaagctacg gcatggacac
    18361 cgcccgtatg ctcggcagca tgatgttcga aggtggtgag atcatccgcg aacgtatcaa
    18421 acgttatcag attgactgcg actaccgccc cggcggtctg tttgtggcga tgaatgataa
    18481 acagctcgcc acacttgaag agcagaaaga gaactgggaa cgctacggca ataaacagct
    18541 ggagatgctg gacgccaacg ccattcgccg cgaggtcgcc agcgatcgct acactggtgc
    18601 gctgctggat cacagcggcg ggcatattca cccgctgaac ctcgccattg gcgaagcgga
    18661 cgctatccgc ctgaacggcg ggcgcgtgta tgagctttcc gcagtgacgc agatccagca
    18721 caccacgccc gccgtagtgc gtacagccac aggtcaggtg acggcgaagt atgtgattgt
    18781 cgccgggaat gcgtacctgg gcgataaagt ggagccggaa ctggcgaaac gcagcatgcc
    18841 gtgcggtacg caggtgatca ccaccgagcg gctttccgat gatttagccc gttcgctgat
    18901 cccgaaaaac tactgcgtgg aagactgtaa ttatctgctt gattactacc gtcttaccgc
    18961 cgacaaccgt ttgctgtacg gcggcggcgt ggtgtacggc gcgcgcgacc cggatgacgt
    19021 tgagcgtctt gtggtgccga aactgctgaa aaccttcccg cagttgaagg gcgtgaaaat
    19081 cgactatcgc tggacgggca acttcctgct gaccctgtcg cgtatgccgc agttcggtcg
    19141 cctcgatacc aacatctatt acatgcaggg ttacagcggc cacggcgtca cctgtactca
    19201 tctcgccgga cgtttgattg ccgaactgct gcgcggcgac gccgaacgtt tcgatgcctt
    19261 cgccaatctg ccgcattacc cgttccccgg cgggcgcacg ctgcgcgtac cgtttaccgc
    19321 gatgggcgcg gcgtattaca gcctgcgcga tcgtctgggc gtttaatttc cgattaacag
    19381 tgaagagtca aaaggtgtga aacatgagca acaatgaatt ccatcagcgt cgtctttctg
    19441 ccaccccgcg cggggttggc gtgatgtgta acttcttcgc ccagtcggct gaaaacgcca
    19501 cgctgaagga tgttgagggc aacgagtaca tcgatttcgc cgcaggcatt gcggtgctga
    19561 ataccggaca tcgccaccct gatctggtcg cggcggtgga gcagcagctg caacagttta
    19621 cccacaccgc gtatcagatt gtgccatatg aaagctacgt caccctggcg gagaaaatca
    19681 acgcccttgc cccggtgagc gggcaggcca aaaccgcgtt cttcaccacc ggtgcggaag
    19741 cggtggaaaa cgcggtgaaa atcgcccgcg cccataccgg acgccctggc gtgattgcgt
    19801 ttagcggcgg cttccacggt cgtacgtata tgactatggc gttaactgga aaggtcgcgc
    19861 cgtacaaaat cggcttcggc ccgttccctg gttcggtgta tcacgtacct tatccgtcag
    19921 atttacacgg catttcaaca caggactccc tcgacgccat cgaacgcttg tttaaatcag
    19981 acatcgaagc gaagcaggtc gcggccatta ttttcgaacc ggtgcagggc gagggcggtt
    20041 tcaacgttgc gccaaaagag ctggttgccg ccattcgccg cctgtgcgac gagcacggta
    20101 ttgtgatgat tgccgatgaa gtgcaaagcg gctttgcgcg taccggcaaa ctgtttgcga
    20161 tggatcatta cgccgataag ccggacttaa tgacgatggc gaaaagcctc gcgggcggga
    20221 tgccgctttc gggtgtggtc ggtaacgcga atattatgga cgcgcccgcg ccgggcgggc
    20281 ttggcggcac ctacgccggg aatccgctgg cggtggctgc cgcgcacgcg gtgctcaaca
    20341 ttatcgacaa agagtcactc tgtgagcgcg ccatgcagtt ggggcagcgt ctgacgaaaa
    20401 ccctgattga tgccaaagaa aacgttccgg ccatcgcggc ggtacgtggt ctgggctcga
    20461 tgattgcggc agaatttaac gacccgcaaa cgggcgagcc gtcagccgcc attgcgcaaa
    20521 aaattcagca acgcgcgctg gcgcagggac tgcttctact gacctgtggc gcatacggca
    20581 acgtgattcg tttcctgtat ccgctgacca tcccggatgc gcaattcgat gcggcaatga
    20641 aaattttgca ggatgcgctg agagattaat acgcagaagc aggggcaacg caataatgcc
    20701 tgatatgctg cgcttatcag acctgcaaac gacgtattga ttatgtatgc cggataaggc
    20761 gttcacgccg catccggcaa gttgtattgc ccaacttcgc taaatctggt gctttttcaa
    20821 caacgcgcgg aactgatgat aggttaagcc cagcaattca gctgcgcgct tctggttata
    20881 tttcccttgt tgcaaactga gttgcagcaa ctctttttcc tgctgcatct gaaactcacg
    20941 taaatccagc ggcagtgttg gaagcgaggt ggtttctgaa acggcgatag cgtcttcagg
    21001 cggacgccgt ttaaagggat caataatgat gtcatcaagc ggataatcgc tggtgccgtg
    21061 gcgatacact gaacgttcca ccacgttttt caattcacga atatttcctg gccaacgata
    21121 attcagcaat gtttctctgg cgcgctccgt aaaccccggg aacagaggca gcttgatttc
    21181 ccgacacatc tggatggcaa agtgttccgc cattaacata atgtcgcttt cacgttcgcg
    21241 tagtggcggc agttgcacta catcgaaagc cagccggtcg agcaggtcag cgcgaaaagt
    21301 accttcattg accatcgccg ggagatcggc attcgtcgcg cataccaacc gcacattcac
    21361 ctgcaacggc tggctgccgc caacgcgctc cagttcaccg tactcaatca cgcgcaataa
    21421 tttctcctgc accatcatcg gtgccgtagc gagttcatca agaaatagcg taccgccatc
    21481 ggcacgttca aatctccctg gatgacgttt ttgcgcaccg gtaaacgccc ccgcttcgtg
    21541 accaaacagt tcggaatcca gcagattttc atttaacgcc gcgcagttaa gggaaataaa
    21601 cggcccttgc caacgggagg agagataatg caggcggctg gcgatcagct ctttaccggt
    21661 gccgcgttcg ccgatgatga gcaccggttt gtccagcggt gcgagatgcg aaacctgttc
    21721 cagcacttcg agaaagctgt tcgcctcacc gagtaaatta tctttgtatt ctgccatgat
    21781 gaaattcgcc acttgttagt gtaattagct aattcatcct ggcatgttgc tgttgattct
    21841 tcaatcagat ctttataaat caaaaagata aaaaattggc acgcaaattg tattaacagt
    21901 tcagcaggac aatcctgaat gcagaaatca agaggacaac attatgggta ttttttctcg
    21961 ctttgccgac atcgtgaatg ccaacatcaa cgctctgtta gagaaagcgg aagatccaca
    22021 gaaactggtg cgtctgatga tccaggagat ggaagataca ttggttgaag tacgttctac
    22081 ttcggcgcgt gcgctggcag aaaagaaaca gctgactcgc cgtattgaac aagcgtcggc
    22141 gcgtgaagtt gaatggcagg aaaaagccga actggcgctg ctgaaagaga gagaggattt
    22201 ggcgcgtgcg gcgttaattg aaaaacagaa actgaccgat ctgattaagt ccctggaaca
    22261 tgaagtgacg ctggtggacg atacgctggc acgcatgaag aaagagattg gcgagctgga
    22321 aaacaaattg agcgaaacac gcgctcgcca gcaggcattg atgttacgcc atcaggcggc
    22381 aaactcgtcg cgcgatgtgc gtcgtcagct ggacagtggc aaactggatg aagcaatggc
    22441 tcgtttcgaa tctttcgaac gtcgtattga ccagatggaa gcggaagcag aaagccacag
    22501 cttcggtaaa caaaaatcgc tggacgatca gtttgccgaa ctgaaagccg atgatgcaat
    22561 cagcgagcaa ctggcacaat taaaagccaa aatgaagcaa gacaatcaat aataacatcc
    22621 aggcggcgtc cgaacgcgcc gccgctcatc gtctaaggag tacttatgag cgcgctattt
    22681 ctggctattc cattaaccat ttttgtgctg tttgttttac cgatctggtt atggctgcat
    22741 tacagcaatc gttctggtcg cagtgaattg tcgcaaagtg agcagcagcg attagcgcaa
    22801 ctggctgatg aagcaaaacg gatgcgcgaa cgtattcagg cgctggaatc tattcttgat
    22861 gcagaacatc cgaactggag ggatcgctaa tggcgggcat taatctcaat aaaaaattat
    22921 ggcgtattcc acagcagggc atggtccgtg gcgtctgcgc cgggattgcc aactattttg
    22981 atgtaccggt aaaactggtg cgtatcctgg tggtgttgtc gattttcttc ggtctggcgc
    23041 tgtttaccct ggttgcttac atcattttgt catttgcgct tgatccaatg ccggacaaca
    23101 tggcctttgg tgagcagcta ccttccagca gtgaattgct ggatgaagtc gaccgtgaac
    23161 tggcggcaag tgaaacgcgt ttacgcgaga tggaacgtta tgtcacttcc gatacattca
    23221 cgttacgtag ccgtttccgt caactgtgag gaaagttatg aatactcgct ggcaacaggc
    23281 cgggcaaaag gtaaagcctg gtttcaaatt agcaggcaag ctggtacttc ttaccgcact
    23341 gcgctatggc ccagcgggtg tggcgggctg ggcgataaaa tcagttgctc gccgaccgct
    23401 gaaaatgttg ctggctgtgg cgctggaacc gctgttaagt cgggctgcca ataaactggc
    23461 gcagcgttat aaaaggtgag gggagtttcg caaaaattgt taaatctcag gcgtataatg
    23521 gatagcaata ttcatccata gaaggacgct tacatgttta aaaaaggctt acttgctctg
    23581 gcactggtgt tttcactgcc cgttttcgcc gctgaacact ggatcgatgt tcgtgttcca
    23641 gagcagtatc agcaagagca cgttcagggg gccatcaata ttcccctgaa ggaagtgaaa
    23701 gagcgcattg ccaccgtcgt tccggataaa aacgacaccg tgaaagtgta ttgcaatgcc
    23761 ggacgccaat cagggcaggc aaaagagatc cttagcgaga tgggatatac ccacgttgag
    23821 aacgccggtg gcctgaaaga catcgcaatg ccgaaggtca aaggttaaaa gaattcacca
    23881 tgagcggcgc ttatgcgccg tttttttctg tcacacctta tttacatccc tatagattat
    23941 ttgcgtcagc tcacaaatac gctttttccc tggtaaaaaa tgatttcctg cgtgactaaa
    24001 acccttgtgc tcaattgaca gtttattttc tgcggagtag tctctcattt catgggaccg
    24061 ctaccaggaa aggcaacatg aaacagaaaa ttacggatta cctggacgaa atctacggtg
    24121 gaacatttac cgcaactcat ttacagaaac ttgtaacgcg tcttgagagt gcgaaacgat
    24181 taattacaca gcgacgtaaa aaacactggg atgaaagtga tgtcgtgtta attacctatg
    24241 ccgatcaatt tcacagtaat gatttaaaac cactacccac atttaatcag ttttaccatc
    24301 aatggctgca aagcattttt tcacatgttc atttattacc tttttatcca tggtcatctg
    24361 atgatggatt ttcggtaatt gattatcatc aggtcgccag tgaagcgggt agtggcagga
    24421 tattcagcag ctcggtgaat gcagtcattt aatgtttgat tttgtctgca accatatgtc
    24481 ggcaaaaagt gaatggttta aaaactattt acaacagcag ccaggttttg aagatttttt
    24541 tattgcggtt gatccgcaaa ccgatctcag cgctgtcacc cgtccgcgtg cattaccgct
    24601 attaacgccg tttcaaatgg atgataattc cacgcgccat ttatggacca cctttagcga
    24661 cgatcaaatt gacctgaatt accgtagccc tgacgtgttg ctggcgatgg tggatgtttt
    24721 gctttgttac ctggaaaagg gcgcagaata tgtccgtctg gatgccgttg gctttatgtg
    24781 gaaagagccg ggaacaagct gcatccatct ggaaaaaaca catctgatta tcaaactgtt
    24841 acggtcgatt attgatgaca tcgcgccagg tacggtgatt attactgaga cgaatgttcc
    24901 gcataaagat aacattgctt actttggcga aggcgatgac gaagcgcata tggtgtacca
    24961 gttctcgctg ccgccgctgg tgttacatgc ggtgcaaaaa cagaacgtcg aggcgctgtg
    25021 ccagtgggcg caaagtctga gcttgccctc cggtaaaacg acctggttta acttcctcgc
    25081 ctctcacgat ggcatcgggc tgaacccact gcggggcttg ttaccagaaa gcgaaatatt
    25141 agcgctggtc gaggcattac agcaggaagg tgcattagta aactggaaaa ataatcccga
    25201 cggtacgcgc agtccctatg aaataaatgt cacctatatg gatgcgttaa gccgccgcga
    25261 gagtagcgat gaagaacgtt gcgccaggtt tatccttgcc catgcgattt tgttaagttt
    25321 ccccggtgtg ccagcgatat atattcaaag tattctgggc tcgcgtaatg attacgcagg
    25381 tgtcgaaaaa ctcggatata accgtgcgat taaccgtaaa aaatatcaca gtgaagagat
    25441 aacccgagaa ctgaacgatg aagctacatt aaggcatgcg gtatatcatg agttgtcgcg
    25501 tttaattaca cttcgtcgca gccataacga gtttcatccg gataataatt ttaccatcga
    25561 tacggttaat tcatccgtaa tgcgtattca aagaagtaac gcagagggta attgtctgac
    25621 cggattgttt aatgtcagta aaaatattca gcatgtaaat attactggcc tgcacggtcg
    25681 ggatctgatt agtgaagttg atatattggg taataaaata acgctgcgcc cctggcaggt
    25741 tatgtggatt aaataaaaag gaacatctca tgattaaatc aaaaatcgtg ctgttatcag
    25801 cactggtttc ctgcgccctg atttcaggct gtaaagaaga aaataaaccc catgtatcca
    25861 tcgaatttat gcattcttcg gtggagcagg agcgtcaggc agttatcagc aaattgattg
    25921 cccgttttga aaaagaaaac ccaggtatca ccgttaaaca agtgcccgtc gaagaggacg
    25981 cttataacac caaagtcatc actctttcac gtagcggctc gctgccggaa gtgattgaaa
    26041 ccagtcatga ctacgccaaa gttatggaca aagagcagct tatcgatcgc aaggcggttg
    26101 ccacggtcat cagcaacgtt ggtgaaggcg cgttttacga tggcgtactg cgtattgtgc
    26161 gtaccgaaga tggtagcgca tggaccggtg ttcctgtcag cgcctggatt ggcggcatct
    26221 ggtatcgcaa agatgtgctg gcaaaagcgg ggctggagga gccgaaaaac tggcaacagc
    26281 tgctggacgt tgcacagaaa ctgaatgacc cggcgaataa aaaatacggt attgcgctgc
    26341 ctacggcaga aagcgtgctg acggaacaat ccttctccca gtttgcgtta tccaactagg
    26401 ccaacgtctt taacgccgaa ggcaaaatca cccttgattc accagagatg atgcaggcgc
    26461 tgacctatta ccgcgacctt gctgccaaca ccatgccggg ttctaacgac atcatggaag
    26521 tgaaagacgc ctttatgaac ggcaccgcgc cgatggcgat ttactccacc tatatccttc
    26581 cggctgtgat taaagaaggc gatccgaaaa acgtcggttt cgtggtgcca accgagaaaa
    26641 actctgccgt ctacggcatg ttgacctcgc tgaccattac cgccggacaa aagactgaag
    26701 aaacggaagc ggctgaaaaa tttgtcacct ttatggagca ggcagacaac attgccgact
    26761 gggtgatgat gtcgccaggc gcggcgctgc cggtgaacaa agcggttgtc actacttcca
    26821 cctggaaaga caacgacgtt attaaggcgc tgggtgaact gccaaatcag ttaatcggtg
    26881 aactgccgaa tattcaggtc tttggcgcag taggggataa aaaatttacc cgcatgggcg
    26941 atgtgacggg gtctggggtg gtgagctcca tggtgcataa cgtcaccgta ggtaaagccg
    27001 atctccctgg cacgctgcaa acgagccaga aaaagctgga tgaactgatc gaacagcact
    27061 aaacccagga caggaatccg caatgaacag gcttttttca ggtcgttccg atatgccctt
    27121 tgcgctgttg cttctcgcac ccagcttatt actgctgggc ggtctggttg cgtggccaat
    27181 ggtgtcgaat atcgaaatta gttttttacg tctgccgctc aatcccaaca tcgagtcaac
    27241 gtttgttggg gtaagcaact atgtgcgtat tctctccgat cccggcttct ggcactcgct
    27301 gtggatgacg gtctggtata ccgcgttggt ggtggcgggc agcaccgtgc tcgggctggc
    27361 agtggcgatg tttttcaacc gcgagttccg cctgcgcaaa accgcgcgtt cgctggtgat
    27421 cctctcctac gtaacgccgt ctatttcgct ggtgttcgcc tggaaataca tgttcaacaa
    27481 cggctacggc attgtgaact acctcggcgt cgatcttctg catctttatg agcaggcacc
    27541 gctgtggttc gataatccgg gcagtagttt tgtgctggtg gtgatcttcg ccatctggcg
    27601 ctacttcccg tatgccttta tctcgtttct ggcgattttg cagaccatcg acaaatcgct
    27661 gtacgaagcg gcagagatgg atggcgctaa tgcctggcaa cggtttcgta tcgtcacgct
    27721 gcccgcgatt atgccggtcc tggcgacggt ggtgaccctt cgcaccatct ggatgttcta
    27781 catgttcgcg gatgtttatc tgctgacaac caaggtcgat attcttggtg tctatctcta
    27841 caaaaccgcc tttgccttta atgacttagg gaaagcggcg gcgatctcgg tggtgctctt
    27901 catcattatt ttcgctgtca ttctgctgac caggaaacgg gtgaacctca atggcaacaa
    27961 ataaacgcac actcagtcgt atcggttttt accgcgggct ggcgctgttt ctcatcatca
    28021 cgctgtttcc gttttttgtg atgctgatga cctcgttcaa gagcgcaaaa gaggcgatct
    28081 cgctgcatcc tacgctgctg ccgcaacagt ggacgctgga gcattatgtc gacattttta
    28141 acccggtgat tttcccgttt gtcgactact tccgtaacag catggtggtg tcggtggttt
    28201 catccgtggt ggcggtattt ctcggcattc tcggagctta cgcgctttct cgcctgcgct
    28261 ttaaaggccg gatgaccatc aacgccagct tttacacggt gtatatgttc tccggcattt
    28321 tgctggtggt gccgctgttc aaaattatca ccgcacttgg catttacgac accgagatgg
    28381 cgctgatcat taccatggtg acgcaaactc tgcccaccgc cgtgtttatg ctgaaaagtt
    28441 acttcgacac catccctgat gagatcgaag aagcggcgat gatggatggc ctcaaccgtc
    28501 tgcaaattat cttccgtatc accgtgcccc tggcgatgtc cgggctgata tccgtattcg
    28561 tctactgctt tatggtggca tggaacgact atctgtttgc gtcgattttc ctctccagcg
    28621 ccagcaattt caccttacca gtcggcctga acgcgctgtt cagtacgcca gattacatct
    28681 ggggacggat gatggcggcc tcgctggtga ccgcattgcc ggtggtgatt atgtatgcgc
    28741 tttcggaacg ttttattaag agtggtttga ccgccggtgg cgtgaagggc taaagcggcc
    28801 agtttcttta caaggagttt tacatgaaaa agttagtagc cacagcaccg cgcgttgctg
    28861 cgctggttga gtatgaagat cgggcgattt tagccaatga agtgaagatc cgcgtgcgtt
    28921 tcggtgcacc gaaacacgga acggaagtgg tcgacttccg cgccgccagc ccgtttatcg
    28981 atgaagactt taacggcgaa tggcagatgt tcaccccgcg tccggcagat gcgccgcgcg
    29041 gcattgagtt tggcaaattc cagcttggca acatggtggt tggcgacatt atcgagtgcg
    29101 gcagcgacgt taccgactac gcggtgggcg acagcgtatg cggctacggc ccgctctccg
    29161 agacgatcat cattaacgca gtgaataact acaagctgcg caaaatgccg gaaggcagct
    29221 cctggaaaaa cgctgtctgc tacgacccgg cgcagtttgc catgagtggc gttcgcgatg
    29281 ccaacgtacg cgtaggggat tttgtagtgg tggtagggct tggcgcgatc ggtcaaattg
    29341 ccatccaact ggctaaacgc gctggcgcgt cggtggtaat tggcgtcgat cctattgccc
    29401 atcgttgtga tattgctcgt cgtcacggtg cggatttctg ccttaatccc attggcactg
    29461 acgtaggcaa agagatcaaa acgctgaccg gcaagcaggg tgccgatgtg attatcgaaa
    29521 ccagcggtta cgccgacgcg ctgcagtcgg cgctgcgcgg cctggcttac ggtggcacca
    29581 tctcctatgt cgcgtttgct aaaccgtttg ctgaaggttt taacctcgga cgcgaagcgc
    29641 atttcaataa cgccaaaatt gtcttctctc gcgcgtgcag tgaaccgaac ccggattatc
    29701 cgcgctggag ccgtaagcgt attgaagaaa cctgctggaa actgctgatg aacggttatc
    29761 tcaattgcga agatttaatc gacccggtag tgacctttgc caacagcccg gaaagctaca
    29821 tgcagtatgt cgatcagcat ccggaacaga gcatcaaaat gggcgtcacg ttttaattca
    29881 aaggaatgaa aaaatgaaaa taggcacaca gaatcaggcg ttcttcccgg aaaatattct
    29941 ggagaaattt cgttatatca aagagatggg cttcgatggt tttgagattg acggcaaact
    30001 gctggtcaac aacatcgaag aagtcaaatc ggcgatcaaa gaaaccggtt taccggtgac
    30061 caccgcctgc ggcggctatg acggctggat tggtgacttt atcgaagagc gtcgtcttaa
    30121 tggcttaaag cagatcgaac gcattctcga agcgctggca gaagtggggg gtaaaggtat
    30181 cgtcgttccg gcagcgtggg gcatgtttac cttccgctta ccgccgatga cctcgccgcg
    30241 tagcctggac ggcgaccgca aaatggtgag tgattccctg cgcgtactgg aacaggtcgc
    30301 cgcgcgtacc ggaaccgtgg tgtatctcga accgttaaac cgctatcagg atcatatgat
    30361 caacaccctc gccgatgctc gtcgttacag cgtcgaaaac aatcttaaac atgtgcagat
    30421 tatcggcgat ttctatcaca tgaacatcga agaagataac ctggcgcagg cgctgcatga
    30481 caaccgcgac ttgctcggtc atgtgcatat tgccgataac catcgttacc agccgggcag
    30541 cggcaccctc gatttccacg cgctgtttga acagctgcgc gccgataact atcagggtta
    30601 tgtggtgtat gaagggcgta tccgggcaga agatcctgcc caggcgtacc gtgattcgtt
    30661 gacctggttg cgtacctgct aagaggtctt tgtgaaaagt gcaatgacaa gctctccact
    30721 gcgggtcgcg ataataggcg caggccaggt ggcggataag gttcatgctt cgtactactg
    30781 cacccgcaac gatctggaac tggtggctgt ctgtgacagc cgcctttccc aggcgcaggc
    30841 gctggcagaa aaatacggga atgcatccgt gtgggacgat ccgcaggcca tgctgctggc
    30901 ggtgaaacct gatgtggtta gcgtctgctc acctaaccgt tttcattacg aacataccct
    30961 gatggcgctg gaagcgggct gccatgtgat gtgcgaaaaa ccgcccgcca tgacgccaga
    31021 acaggcgcgg gaaatgtgcg ataccgcgcg caaacagggc aaggtgctgg cctacgactt
    31081 tcaccatcgt tttgcgctcg atacgcaaca gctgcgtgaa caggtgacca acggcgtttt
    31141 gggagagatt tacgttacca ccgcccgcgc cctgcgtcgc tgcggcgttc ccggctgggg
    31201 tgtctttacc aataaagaac tgcagggtgg tggcccgctg atcgacatcg gcattcatat
    31261 gctggatgct gcgatgtatg tgctgggcta tcctgcggtg aaaagcgtga atgcgcatag
    31321 ctttcaaaag atcggcacgc aaaagagctg tggtcaattt ggcgagtggg atccggcaac
    31381 ttacagcgtc gaagattcgc tgtttggcac cattgaattt cataacggcg gcattctgtg
    31441 gctggaaacg tcatttgcgc tcaacatccg cgaacagtcg attatgaacg tcagcttttg
    31501 tggtgataaa gccggtgcga cgctgtttcc agcacatatc tacaccgata acaacggtga
    31561 attaatgacg ctgatgcaac gggaaatggc agacgacaac cgccatttgg gcagcatgga
    31621 agcctttatc aatcacgtac agggcaagcc cgtgatgata gccgacgccg agcaggggta
    31681 catcatccag caactggtgg cggcgttata tcaatccgca gaaacaggga cgcgtgtgga
    31741 attatgacca ggccagtaac gttatcagaa ccccatttca gccagcatac cctgaacaag
    31801 tatgcatcgc tgatggcgca ggggaacggc tatcttgggc ttcgcgccag ccatgaagaa
    31861 gattacacgc gccagacgcg agggatgtat ctggcggggc tgtatcatcg ggcgggaaaa
    31921 ggtgaaatca acgaactggt gaacctgcct gatatcttgg ggatggagat tgccataaat
    31981 ggtgaggttt tctcgttatc ccacgaagcc tggcagcgtg agcttgactt tgccagtggc
    32041 gaattacgcc gcaacgttgt ctggcgtacc agcaacggcg caggttacac catcgccagc
    32101 cgtcgctttg tttcggcaga ccaactgccg ctcattgcgc tggaaatcac tattacgcca
    32161 ctggacgccg acgcgtcagt gctgatttca acaggtatcg acgccacgca aaccaaccac
    32221 ggtcgccaac atctcgacga aacccaggtg cgggtgtttg gtcagcatct gatgcagggg
    32281 atctacacca cccaggatgg acgcagtgat gtggccatca gctgttgctg taaggtgagc
    32341 ggtgatgtgc agcaatgcta taccgccaaa gagcgccgtt tgctgcaaca taccagtgcg
    32401 cagcttcatg caggcgagac agtgacgttg caaaaactgg tgtggatcga ctggcgggat
    32461 gacaggcaag ccgttttaga cgagtggggc agcgcgtcgc ttcgccagct tgaaatatgc
    32521 gcgcagcaga gttacgacca acttcttgca gcatcaacag aaaactggcg tcaatggtgg
    32581 cagaaacgtc gtatcacggt aaatggcggc gatgcgcacg atcagcaagc gttagattat
    32641 gcgctttatc atctgcgcat catgacgccg gctcacgacg agcgcagcag tattgcggca
    32701 aaaggcttaa ccggcgaagg ctacaaaggc cacgttttct gggatacaga agtatttttg
    32761 ctgccgttcc atctgtttag cgatccgacg gttgcccgaa gtttactgcg ttatcgctgg
    32821 cacaacttgc caggcgcgca ggagaaagca cggcgcagcg gctggcaggg cgcgctattt
    32881 ccgtgggaaa gcgcgcgcag cggcgaagaa gagacgccag aatttgccgc cattaacatt
    32941 cgtaccgggc tgcggcaaaa agtggcctcg gcgcaggcgg aacatcatct ggtggccgat
    33001 atcgcctggg cggttattca atactggcag accacggggg atgaaagttt cattgctcat
    33061 gaaggcatgg cgctacttct ggaaactgca aagttctgga ttagccgcgc ggtgagggtt
    33121 aacgaccgtc tggaaattca tgatgttatt gggccagacg aatataccga acatgtcaat
    33181 aataacgcct tcaccagcta tatggcccgc tacaacgttc aacaggcgct gaatattgcc
    33241 cgccagttcg gctgtagcga cgatgcgttt atccatcgcg ccgaaatgtt cctcaaagag
    33301 ctatggatgc cagaaacgca gcccgatggc gttttgccgc aggatgattc gtttatggct
    33361 aagccggcga ttaatctggc taaatacaaa gcggcggcgg ggaagcaaac cattctgctg
    33421 gattattcac gcgcagaagt gaacgagatg cagatcctca aacaagctga tgtggtgatg
    33481 ctcaattaca tgctgccgga gcagttctca gcggtatcgt gtcttgccaa tctgcaattt
    33541 tatgaaccgc gcactattca cgactcgtca ttaagtaaag caatccacgg cattgttgcc
    33601 gcacgctgtg gcctgctgac ccaaagttat cagttctggc gcgaggggac tgaaatcgat
    33661 cttggtgctg atccgcatag ttgtgatgat ggtatccacg ctgccgcaac tggcgctatc
    33721 tggctggggg cgattcaggg ttttgccggg gtgagcgtgc gtgacggtga attacatctc
    33781 aatccggcgt taccggagca gtggcaacag ttgtctttcc ctctgttctg gcagggctgc
    33841 gaattacagg tcacgctcga cgcgcagcgt attgcgattc gaacttctgc gcccgtttca
    33901 ctgcgtttga acgggcagct tatatccgtg gctgaagaat ctgttttctg tttgggtgat
    33961 tttattttgc ccttcaatgg gaccgctacc acgcatcagg aggatgaatg aaactgcaag
    34021 gggtaatttt cgatctggat ggtgtaatca ccgataccgc gcatctgcat ttccaggcgt
    34081 ggcagcagat tgccgctgaa attggcatca gcattgatgc gcagtttaac gaatccctaa
    34141 aagggatcag ccgcgatgag tctctgcggc gcattctgca acacgggggc aaagagggcg
    34201 actttaaccc gcaggagcgg acgcaactgg cgtatcgcaa aaatctgctt tacgtccact
    34261 cactgcgcga gctgacggtt aatgctgtgc tgcccggtat tcgcaatttg ctggcagagc
    34321 tccgcgcaca gcagatcccg gtagggctgg cttctgtctc cctgaatgcg ccgacgattt
    34381 tagcggcgct ggagctgcgc gagtttttca ccttctgcgc ggatgcttcc caacttaaaa
    34441 actcgaaacc ggatccggaa atctttctcg ctgcctgtgc cgggctgggc gtgccgccgc
    34501 aggcatgtat cggcattgaa gatgcgcagg cgggcattga cgccattaac gccagcggta
    34561 tgcgctcggt ggggatcggc gcaggcttaa ccggggcgca attattgttg ccctcaacgg
    34621 actccctgac ctggccgcgg ttatcggcct tctggcaaaa cgtatagcaa aggaatcaac
    34681 atggctcagc tttcgttaca acatattcaa aaaatctacg ataaccaggt gcatgtggtg
    34741 aaggacttca acctggaaat tgccgataaa gagttcatcg tatttgtcgg cccgtcgggc
    34801 tgcggtaaat cgaccaccct gcgcatgatt gccgggcttg aggagatcag cggcggcgat
    34861 ctgttgatcg acggcaaacg aatgaatgac gttccagcca aagcacgcaa tatcgcaatg
    34921 gtgttccaga actacgcgtt gtatccgcat atgacggttt acgacaacat ggcgtttggc
    34981 ctgaagatgc aaaaaatcgc caaagaggtg attgatgagc gggtgaactg ggcggcgcaa
    35041 attctcggcc tgcgtgagta cctgaagcgc aagccgggcg cgctttccgg cgggcaacgc
    35101 cagcgtgtgg cgctcgggcg ggcgattgtg cgcgaagcgg gcgtgttttt aatggatgaa
    35161 ccgctctcta accttgatgc caagctgcgc gtacaaatgc gcgcagagat cagcaagctg
    35221 catcagaaac tgaacaccac catgatctac gtgacccacg atcagaccga agcgatgacc
    35281 atggcgacgc ggattgtgat tatgaaagac gggattgttc agcaagtggg tgcgccgaaa
    35341 accgtttata accaacccgc gaatatgttt gttgccggat ttattggatc accagcgatg
    35401 aattttattc gcggcacgat cgatggcgat aaattcgtta cggaaacgct taaattaacc
    35461 attcccgaag agaaattagc ggttctgaaa acacaggaaa gtttgcataa gcccatcgtg
    35521 atgggaatgc gcccggaaga tattcatccg gacgcgcaag aggaaaataa tatttccgcc
    35581 aaaattagcg tggtagaatt aaccggtgcg gaatttatgc tctacaccac ggttgggggg
    35641 cacgagttag tggtccgtgc tggtgcgtta aatgattatc atgcaggaga aaatatcact
    35701 attcattttg atatgactaa atgtcatttc tttgatgcag aaacggaaat agcaattcgc
    35761 taaatacagg gggaaggcat tcccccagga taatacaagg aacaataatg aaaaagttat
    35821 taccctgtac cgcactggtg atgtgtgcgg gaatggcctg cgcacaggcc gaggaaaaga
    35881 acgactggca ctttaatatc ggcgcgatgt acgaaataga aaacgtcgag ggttatggcg
    35941 aagatatgga tgggctggcg gagccttctg tctattttaa tgccgccaac gggccgtgga
    36001 gaatttctct ggcctattat caggaagggc cggtagatta tagcgcgggt aaacgtggaa
    36061 cgtggtttga tcgcccggag ctggaggtac attatcagtt cctcgaaagc gatgatttca
    36121 gtttcggcct gaccggcggt ttccgtaatt atggttatca ctacgttgat gaaccgggta
    36181 aagacacggc gaatatgcag cgctggaaaa tcgcgccaga ctgggatgtg aaactgactg
    36241 acgatttacg tttcaacggt tggttgtcga tgtataaatt tgccaacgat ttgaacacga
    36301 ccggttacgc tgatacccgt gtcgaaacgg aaacaggtct gcaatatacc ttcaacgaaa
    36361 cggttgcctt gcgagtgaac tattatctcg agcgcggctt caatatggac gacagccgta
    36421 ataacggtga gttttccacg caagaaattc gcgcctattt gccgctgacg ctcggcaacc
    36481 actcggtgac gccgtatacg cgcattgggc tggatcgctg gagtaactgg gactggcagg
    36541 atgatattga acgtgaaggc catgatttta accgtgttgg tttattttac ggttatgatt
    36601 tccagaatgg tctttccatt tcgctggaat acgcgtttga gtggcaggat cacgacgaag
    36661 gcgacagcga taaattccat tatgcaggtg tcggcgtaaa ttactcgttc tgataatggg
    36721 ctaaattgcc ggatgcggcg cgagtacttt atccgatcta taaatgtagg ccggataaga
    36781 tgcgccagca tcgcatccgg cattcaggca aggtcgctgg tatttatttc agcgtcatat
    36841 gcgtggcaac ggtaatattt tgtggtgacg gttttccggc aattaagcgg aataataact
    36901 cacagctttg ttgacctaac tcctgcgtcg gaacatcgat gccgcccggt gcaggcgtta
    36961 aaataaatga cagcgtttca ttgctataac ccaccaccgc taactgctgc ggaatagcaa
    37021 tgtttttctc tgccgccgca cgataaatgc tcattaattt caggctgtca gtggcaaaca
    37081 ctgcctcagg caacggtgac tgacttaata attgccgtgc tgcttgcagt gctgtttcat
    37141 gggtataacc gccatcaaca atccattcat cacgcactgc aatattatga tcttccagac
    37201 tctgcttata accatttacc cgatccactg aaacatggac atcaagcggt gcatgcagac
    37261 aggcaatatt ttgatgtcca ctttcaatta acgcatcggt caacgcaatg ctgtcaccaa
    37321 aattatcggt atcgacagaa taaacatggg catattgacc ttcaactttg ccaatcacca
    37381 ccacaggaat atcgtattta tcaagttggg caaaaaatga ctcatccgct ggcgaactta
    37441 gcataataat gcctttaatc attttctgct taattttgct ttcacatttt tgtaagtctt
    37501 ctgccggggt gtgcgacgtc tgcaatatca cgtcgaaacc ttcttcttca gctttggcgg
    37561 tgatggcatg caaaacttca gagaaaaacg gattacccgc cgtagttttg gtcgaacggg
    37621 tagaaatcac cataatggca tcaaaacccg aagaggtcag cgcgcgggcc agcttgtttg
    37681 gttgatactg taattcttca atggcccgta acactttttc gcgcgcttcc ggggagatat
    37741 tggtttgctt attcagcacg cgtgatacgg tggattttga tacgcctgca accctggcaa
    37801 tatcataaat agtaggggac ataggtcagg aactccgtcc gaacgtgaat gctgcacatc
    37861 ttatggagtt cgcccgtcga tgacaacacg ggtagttgat aatcaatggc ctggccccca
    37921 cattcatatc cttatgaatg atttttttct ggtcttcaga gcagcacagg acagcaatga
    37981 agcgacttaa aaatgaactt aatgcgctgg tgaatcgggg tgtcgacaga catctgcgcc
    38041 tcgccgtaac cggacttagc cgcagcggca aaacggcgtt tatcactgcg atggtcaatc
    38101 agttgctcaa tattcatgcc ggagcacgtt tgccgctatt aagtgcggtg cgtgaagagc
    38161 gtctgctggg cgtaaaacgc attcctcagc gtgactttgg cattccgcgt tttacctacg
    38221 acgaagggtt ggcgcagctg tatggcgatc ctcccgcctg gccgacgcca acgcgcggcg
    38281 tcagcgaaat tcgcctggca ctacgctata aatcgaacga ttcgctgctg cgccacttta
    38341 aggatacctc cacgctgtat ctggagattg tggattaccc tggcgaatgg ttgctcgacc
    38401 tgccgatgct ggcgcaggac tatttaagct ggtcgcgcca gatgacgggc ttactcaatg
    38461 gtcagcgcgg cgaatggtcg gcgaaatggc gaatgatgtg cgaagggctg gacccgctag
    38521 cgcctgccga cgaaaaccgg ctggcagaca ttgccgccgc gtggaccgat tatctccacc
    38581 actgtaaaca gcaggggctg cactttattc agcctgggcg ctttgtcttg ccgggggata
    38641 tggcaggtgc gcccgcgctg caattcttcc cgtggccgga tgtcgatgcc tggggcgagt
    38701 ccaaactggc gcaggccgat aagcacacca atgccggaat gctgcgcgag cggtttaatt
    38761 attactgcga gaaggtggtg aaggggttct ataagaatca ttttctgcgc tttgaccgcc
    38821 agattgtgct ggtggattgc ctgcaacctc tcaacagtgg gccacaggca tttaatgata
    38881 tgcgtctggc gctgacgcag ctgatgcaaa gtttccacta tgggcagcgt accctgttca
    38941 ggcgtttgtt ttcgccggtt atcgataagc tattgtttgc tgccactaaa gcggaccatg
    39001 tgaccatcga tcagcacgcc aatatggttt cattgctgca acaactaatt caggatgcct
    39061 ggcaaaatgc ggcgttcgaa gggatcagca tggattgcct ggggctggcg tcagttcagg
    39121 cgaccaccag tggcattatt gatgttaacg gtgagaaaat cccggcgctg cgcggtaatc
    39181 gacttagcga tggcgcaccg ctcactgttt atcctggcga agttcccgca cgtttgcctg
    39241 gtcaggcgtt ctgggataag caagggttcc agtttgaagc gtttcgcccg caggtgatgg
    39301 atgtcgacaa accgctgccg catattcgtc ttgatgccgc gctggaattt ttaataggag
    39361 ataaattgcg atgaccgaac cgttaaaacc acgtattgat ttcgacggtc cgctggaggt
    39421 cgaacagaat ccaaaattca gggcgcagca gacctttgac gaaaatcagg cgcaaaattt
    39481 tgccccggcc acgctcgacg aagcgcagga agaagagggg caagtcgaag cggtaatgga
    39541 cgcagcgtta cgtccgaaac gcagcctgtg gcgcaaaatg gtgatgggcg ggctggctct
    39601 gtttggcgca agcgttgtcg ggcagggtgt acagtggaca atgaatgcct ggcaaactca
    39661 ggactgggtg gcgctgggtg gatgtgctgc tggggcattg attatcggcg ctggcgtagg
    39721 ttctgtggta acagagtggc ggcgcttatg gcgcttgcga cagcgcgccc atgaacgcga
    39781 cgaagcgcgc gatttgttgc acagccacgg cacgggcaaa ggccgcgcat tttgcgaaaa
    39841 actggcgcag caggcgggta ttgatcagtc tcatccagcg ctgcaacgct ggtatgcctc
    39901 aatccatgaa acgcagaacg atcgtgaagt ggtcagcctg tatgctcatc tggtccagcc
    39961 ggttttagat gcccaggcgc ggcgcgaaat cagccgctca gcagctgaat caacgttgat
    40021 gattgcggtc agcccgctgg cgctggtgga tatggcattt atcgcctggc gcaatctgcg
    40081 tttgattaat cgcatcgcca cgctgtatgg cattgaactg ggatattaca gccgtttgcg
    40141 cctgttcaag ctggtattgc tgaatatcgc tttcgccgga gccagcgaat tggtgcgcga
    40201 agtgggaatg gactggatgt cgcaagatct cgctgctcgt ttgtctaccc gcgcagctca
    40261 ggggattggt gcaggacttc tgacggcacg actggggatt aaagctatgg agctttgccg
    40321 cccgctgccg tggattgacg atgacaaacc tcgcctcggg gatttccgtc gtcagcttat
    40381 cggtcaggtg aaagaaacgc tgcaaaaagg caaaacgccc agcgaaaaat aatgcaatat
    40441 cgggtgctga ccggatatct ttacgccgaa gtgcccgttt ttccgtcttt gtgtcaatga
    40501 ttgttgacag aaaccttcct gctattcaaa tagtgtcata tcatcatatt aattgttctt
    40561 ttttcaggtg aaggttccca tgcgtctgga agtcttttgt gaagaccgac tcggtctgac
    40621 ccgcgaatta ctcgatctac tcgtgctaag aggcattgat ttacgcggta ttgagattga
    40681 tcccattggg cgaatctacc tcaattttgc tgaactggag tttgagagtt tcagcagtct
    40741 gatggccgaa atacgccgta ttgcaggtgt taccgatgtg cgtactgtcc cgtggatgcc
    40801 ttccgaacgt gagcatctgg cgttgagcgc gttactggag gcgttgcctg aacctgtgct
    40861 ctctgtcgat atgaaaagca aagtggatat ggcgaacccg gcgagctgtc agctttttgg
    40921 gcaaaaattg gatcgactgc gcaaccatac cgccgcacaa ttgattaacg gctttaattt
    40981 tttacgttgg ctggaaagcg aaccgcaaga ttcccataac gagcatgttg ttattaatgg
    41041 acagaacttc ctgatggaga ttacgcctgt ttatcttcag gatgaaaatg atcaacacgt
    41101 cctgacaggt gcggtggtga tgttgcgatc aacgattcgt atgggccgtc agttgcaaaa
    41161 tgtcgccgcc caggacgtca gcgccttcag tcaaattgtc gctgtcagcc cgaaaatgaa
    41221 gcatgttgtc gaacaggcgc agaaactggc gatgctaagc gcgccgctgc tgattacggg
    41281 tgacacaggt acaggtaaag atctttttgc ctacgcctgc catcaggcaa gcccaagagc
    41341 gagcaaacct tacctggcgc tgaactgtgc gtctataccg gaagatgcgg tcgagagtga
    41401 gctgtttggt catgctccgg aagggaagaa aggattcttt gagcaggcga acggtggttc
    41461 ggtgctgttg gatgaaatag gtgagatgtc accacggatg caggcgaaat tactgcgttt
    41521 ccttaatgat ggcactttcc gtcgggttgg cgaagaccat gaggtgcatg tcgatgtgcg
    41581 ggtgatttgc gctacgcaga agaatctggt cgaactggtg caaaaaggca tgttccgtga
    41641 agatctctat tatcgtctga acgtgttgac gctcaacctg ccgccgctac gtgactgtcc
    41701 gcaggacatc atgccgttaa ctgagctgtt cgtcgcccgc tttgccgacg agcagggcgt
    41761 gccgcgtccg aaactggccg ctgacctgaa tactgtactt acgcgttatg cgtggccggg
    41821 aaatgtgcgg cagttaaaga acgctatcta tcgcgcactg acacaactgg acggttatga
    41881 gctgcgtcca caggatattt tgttgccgga ttatgacgcc gcaacggtag ccgtgggcga
    41941 agatgcgatg gaaggttcgc tggacgaaat caccagccgt tttgaacgct cggtattaac
    42001 ccagctttat cgcaattatc ccagcacgcg caaactggca aaacgtctcg gcgtttcaca
    42061 taccgcgatt gccaataagt tgcgggaata tggcttaagc cagaagaaga acgaagagta
    42121 agcgcgaata tgcctgatgg tgcaacacca tcaggcatat taacttatgc tttcagtaca
    42181 gccagagctg cttcgtaatc cggctcggtg gtgatttcat ccaccagctg gctgaaaatc
    42241 acattgtcat tttcatcaat aaccacaacg gcacgcgctg ccagaccttt cagtgggcca
    42301 tcagcaattg ccacaccgta ggcttgcaga aattcagcgt tacggaaagt ggagagggtg
    42361 ataacgttgt tcagaccttc tgcgccgcag aaacgagact gggcgaacgg cagatcggca
    42421 gagatacaca gcacaacggt gttgtcgatc tcggttgcca gttggttaaa cttacgtact
    42481 gatgcggcgc aaacaccggt atcaatactc gggaaaatgt tcagcacttt gcgtttaccc
    42541 gcaaactgac cgagggtgac gtcagacaga tcttttgcca cgagagtaaa agtctgcgct
    42601 ttgctacccg cctgcgggat ggaattggcg actgtaaccg ggttgccctg gaaatgaacg
    42661 gtttgtgaca tgattatctt tcctgtttac atatagttaa cgtcacacct agtttatgct
    42721 aactgtcaat aacacagcaa acgctatttg cgcttaatcc gcagaccacc gcgacaccaa
    42781 ggagtaaaga tgagaaccgt taaggtattt gaggaagcct ggcccttaca taccccgttt
    42841 gtgattgccc ggggaagtcg cagtgaagcg cgcgtggtgg tggttgaact ggaagaagag
    42901 ggtattaaag gcaccggcga atgcacgccg tatccgcgtt atggagaaag tgatgcctcg
    42961 gtaatggcgc aaattatgag cgtcgtgccg caattagaga aagggctgac acgggaggag
    43021 ttgcaaaaaa ttctccctgc cggcgcagca cgtaatgcgc tggattgtgc attgtgggat
    43081 ctggccgcgc gaaaacagca gcaatcgctg gctgatttga tcggcataac gcttcccgag
    43141 acagttatca ctgcacagac ggtagtcatc ggtacgcctg atcagatggc caatagtgca
    43201 tcaacactct ggcaggccgg cgcgaaatta ctgaaagtga agctggataa ccatcttatc
    43261 agtgagcgga tggtggcaat tcgcacagct gtgcccgata ccacgctgat cgttgatgca
    43321 aatgaatcct ggcatgcaga agggttggcg gcacgttgcc agctattggc ggatttaggc
    43381 gttgcgatgc ttgaacaacc gcttcctgcg caggacgatg cggcgctgga gaattttatt
    43441 catccgttgc cgatttgtgc tgatgaaagt tgtcatactc gtagcaattt gaaggcgctg
    43501 aaagggcgct atgagatggt taatattaag ctcgataaaa ccgggggtct gacggaagcg
    43561 ctggcgctgg cgactgaagc gcgtgcacaa ggtttcagtc tgatgctggg ctgcatgttg
    43621 tgtacctctc gggccattag cgccgcttta ccgctggtgc cgcaggtcag tttcgccgat
    43681 cttgacggac cgacctggct ggcggtagat gtggaaccgg cgcttcagtt cacgacgggc
    43741 gaattgcatc tttaggatgc cagcgcagca agtttgccat cgcaaagaga tatttttcgc
    43801 tggcttcgtc ggaggagatg ggcggaaact ctgcggtgat gcaatgcaag ttcagatctg
    43861 cacaccaact gccgaaagag ccaggcgttt cataaccgac gctggttacc agaggcaatt
    43921 caaacgcctg agccagccat tcgcctaatt cgctgtgtct gggatcttca atacaggcca
    43981 gtggatcgtg gaatgagacc acccaggcag gttgaatgcg gtgtataagc tggcacagcg
    44041 cctgggtttc aggttcggag ccaggtttgt cacctgtcag taatacgaca tcacgttctt
    44101 cagcggcgct gttccagcga taaaccgttt caccttcctt ccagttcgcc gccggaaagt
    44161 ttctgtttaa atccacacca ttagcattgg cccgtaaccc cagctggcag ccgtcaggat
    44221 tcacacacag caccacatga tggcggcgca aagaaggtgt caaagtccgc agagcacagg
    44281 agagcgtgac gaccgaagag ttttcatcac cgtgagtgcc agccagaatc aaaccacttt
    44341 cgcgactggc tgcaggggcc ggaaaccaga ttaacggcgc gcccaataat gaacgtccgt
    44401 aatgttctgt tccgggcgga aatgcgccgc gttcggcgcg tgggcgggtt acggtcatga
    44461 tcgtctctgg ttcaggaagt attacaggca gtgttgtgca aattttgcgg attatcaaat
    44521 ccttttcttc aacgctatgt catcgatctt gtatcacttt tttgccggaa gttgtttgta
    44581 ttttcattga tgtgcagata ttagaattgt cgtaatgtta tatacatggc gtattttatt
    44641 cttactggtt ctacacatgt taatcgcatc tgattgatca gttcacctta ataagtttaa
    44701 taaatcgtac tgcacatagt acagtacgat ggtggttata taattgattt acatgaagaa
    44761 tggcttgtcg ccatcagtcc cgggttgatt gatgcctata ttctcaccaa tgtgtttctc
    44821 aggtttatcg atgatatctg taattaatgc tgcgacactc ttaggtgata cgatcgtacc
    44881 tttgaatggt tcattgcgtg aggtaagctc ataatcaata atatcttcat ccgtcagcca
    44941 ggcagggcga agaattgtat attccagtcc agaagcctca attgcatccg cagccctgcg
    45001 gaatggtttt aggggctcac caataactgc attattccat tcgccaaatt ttcctggaac
    45061 ttcatcataa atgccgaggg aaagaacaaa gatcagacgt ttaacatcac aggctttcat
    45121 cgcagcgatc actgaatttg cctgaatatc taaatcttcc cccgtaaggt tggcatacac
    45181 aatatcctgt ccctgcatgg cctgcttcag tgctgcatga ttcagtacat cccccataat
    45241 aatctggcta ttagttggat agggtttgtg tatttttgct ggctgacgag caaataaagt
    45301 ttgtttaatg gtttgtttat ctgccagttg attaatcaca tggcgggcta tctgaccgcc
    45361 agcaccaaga atgagtacat ttttcataat atttctcatg aaacttatca cagatgggtg
    45421 ttgaaaaaag gaataacttt ttccagcgcc tcctgaactg cttccggttt gtcatacagt
    45481 tcataatgtg agaaatcggg taatgaaact aactgacgat cctgcgaaac catcgcccgg
    45541 ccatagactt ccagtccatc acgataagca ccgaaggcac cgactttttc accaactact
    45601 accatcaccg gctgagtcag taacacttca gtaaatgcga aggcatccca ggccagcgtt
    45661 ttctgtgcat gtgagaacaa catttttgtc gcacctcccg gttgttgccc gcgtggtgtt
    45721 ttatagtagt cagttgcttc ataaacatcg cgttcggtca gtccatgggc ttttgctgca
    45781 tccaggctgg cgggaagtaa ttcgttgatc tgtaattctc caccacgagc ctctttggtt
    45841 cgttgcgacg ccattgcatt caatgcgcca atcggatcgt aattgctgaa accttcccga
    45901 aaaagacgcc cgatattaac acccgtaata ctcactacag ctttaattcg tttttctgtc
    45961 agcgcggcat taatggcata gccacccccc ccacacacac acccagcacg ccaatgcgtt
    46021 cagcatcaac atatggcaga gttactgcat aatcgataac acggctaata tcctctacgc
    46081 gctgtgtcgg atcttctatc caacgaggct ccccaccaga ttctccctgg aaactggcgt
    46141 cataagcgag aacgacatag cctttttcag ccaatgcctt gccatagata ttgccagacg
    46201 tctgctcctt acaactgcca aaaggatgga cgctgataat cgccggatac tgttttgact
    46261 catcaaaatt tggtggaaac tgaatattgg cagcaatatg ccaggccata tcactattgc
    46321 ggatttgaat atgtttcatt acagagtttc ctcaaagaac ggagctaata ctgaaattgc
    46381 ttcagccaca taggctttgc cgtcataaag atccatatgg tttgcgcctt caacaatgtg
    46441 atagcgttta tcctggcttg atgcccgatc gtacaggtcg tcactcatcc atttgctccc
    46501 tgcctggctg cccgccacaa tctgcgtcgg ctgagtcagg tacacttccg ccatatggta
    46561 agcatcatag gtaataatct ggttaaggct gcgcaaagta gcgtaacccg gtgctgttgg
    46621 atactgcgcg cgaggggtgt ggtaatactc ccaggcctga cgcagttctt cgttcggcgc
    46681 atcggactcc ttcattggtg ccagtggcat aatggcgtat tctccgctgc taatatcgct
    46741 ggttctggcg tttgaacccg cttcaacgta tggcagggca gcaatagatt tcacattgtt
    46801 ttcccaacca ttacggaaca tcgaaccaat attgaccgca ctaacggtac cgatggcctt
    46861 gatgcggcga tcctgaattg cagcattggc tgtatatcct gcaccggcac aaattcccat
    46921 cgcaccaatt cgggtattgt cgacataaga aagcgttgtc aggtaatcaa tcacggcact
    46981 gacgtcttca gtacgaatgt atgggttttc taactgacgc ggctcgccgc cactttcacc
    47041 ctgataagat gcgtcataag caatagtgac aaatcccttt tccgccagtt tttcggcata
    47101 ggtcccggcc gtttgttctt taacgccccc acctgggtga gataccacaa ttgccggata
    47161 ttgacgggtt tcatcaaatt ttggaggaaa atagatcact gcagacaaag agatggttgg
    47221 attattgctg ttagtgaagc tgactttatt attcatcatt ccgttcctca tggagttgtt
    47281 ggttcgtttt aacggttggt gatatcacta tagatattga tcattaagtt gattagaccc
    47341 taaatcatga ttagacctat catttaaatg attaatagat tgtatggaga tactgaatga
    47401 agcgtgaaga gatcgctgat ctgatggcgt ttgtcgtcgt tgcagaggag cgtagcttca
    47461 ctcgcgcagc agcccgcctg agcatggcgc agtcagcttt aagccagata gtgcgccgta
    47521 tagaagaacg attgggattg cggcttctga cgcgaaccac gcgcagtgtt gtacccactg
    47581 aagcgggcga gcatcttttg tctgttcttg gccctatgct gcatgacata gattctgccc
    47641 tggcatccct gagcgatctg cagaaccgcc catccgggac aatacgtatt actactgtag
    47701 aacatgcagc aaaaacgata ttgttaccag ccatgcgcac attcctgaaa tcacaccctg
    47761 aaattgatat tcagcttatc attgattatg gtttgactga tgtcgtttct gaacgttttg
    47821 atgcaggcgt ccgtctgggt ggggagatgg ataaagatat gatcgccatt cgaatcgggc
    47881 cagatatacc aatggctatt gttggctcac cggattattt ttctcgccga agtgttccaa
    47941 cgtcagtgtc acaattaata gatcatcagg caattaattt gtatcttccc acatcgggta
    48001 cagcaaatcg ctggagatta atacgcggtg gacgtgaagt tcgtgttcgc atggaaggtc
    48061 agcttttact gaatacgata gacctgatca ttgatgctgc aattgatggg catggattgg
    48121 cgtatctacc ttatgatcag gttgagcggg cgattaaaga aaaaaaactg atacgtgttt
    48181 tggataaatt cacaccagat ttacccggtt atcacctgta ctatccacac cgtcgacatg
    48241 ctggctcggc attctcatta tttatagata ggctgaagta taaaggtgct gtttagcact
    48301 acttgctgat acattaattt aattcttctc ttaacgtatt ctcagttcct ttcaacgttt
    48361 tggttatttt ttattcttcg tacaatggcg acagatgctg attatgatac cgaaaactgg
    48421 tttgaacgtg tgaagcccga acgtagtgtt cggtgttcta tgtgctttgc cgcattttgg
    48481 cgtctattat ttacactaaa tctgatttaa tatattgata cataaaacat tttatgcttc
    48541 ctgtgtcact ttctgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc
    48601 atcaatttta atgtcggtta gagggaaact tatgaagcac tctgtttcag tcacgtgttg
    48661 tgcgctgttg gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt
    48721 actggcagag aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc
    48781 cgctaaagcc gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggactggt
    48841 gaatcagaac gagaaagggg agattgtccc cggcgttgcg actcagtgga aaagtaatga
    48901 caaccgtatc tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt
    48961 aacggcgcaa gattttgtct acagctggca gcgtctggtg gacccgaaaa cgctatcgcc
    49021 atttgcatgg tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa
    49081 agctacacct gaccagcttg gcgtcaccgc agttgatgtc catactttga aaattcagct
    49141 tgataaaccg ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca
    49201 aaaagccaac gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg
    49261 cgcttatgtt cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca
    49321 ttattgggat aacgccaaaa cggtactgca aaaagtgacc ttcctgccaa ttaatcagga
    49381 atccgcagcc actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa
    49441 aaatatgtat cagaagctgt tgaaggatat tccggggcag gtttatacgc cgccgcagct
    49501 cgggacctat tattatgcgt ttaacacgca aaaagggccg acggcagatc agcgcgttcg
    49561 tctggcatta agtatgacga tagatcgccg cctgatgacc gaaaaagtat tagggacggg
    49621 cgaaaagcca gcgtggcatt ttacaccaga tgttaccgcc ggatttacgc cggaaccttc
    49681 gccgtttgaa caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc
    49741 agctggttat ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa
    49801 ccatcaaaaa attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt
    49861 taaattgcaa aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga
    49921 tgttatccgc gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt
    49981 aacgtcaacg cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct
    50041 ggcccaggca tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga
    50101 aaaaatcctc atggagcaag caccgattgc accaatttat caatatacca atggacgatt
    50161 aatcaaaccg tggctgaaag gttatcccat taataaccct gaggatgtgg cgtacagtcg
    50221 gactatgtat attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg
    50281 ccagtgatgt ctgatttagc gaccctgttc cggcggtgta atgttatcca tatacagcgt
    50341 ctggctgggg aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata
    50401 aacgtcttgc tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac
    50461 cataatattc aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg
    50521 gtcgatggcc gggtgatttt tcagcatctc acgtacggct tcgacgataa cgcccacttt
    50581 tgccgcatcc tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc
    50641 tgggttttct acgctgatcg atgaaaacag cgagttcggt acgtacaatg gacgattatc
    50701 aaaggtcgta attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct
    50761 gtccggtgaa cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc
    50821 ggaaaagaaa ttactcagaa tatctttgcc agccataccg acagccagac caccaatacc
    50881 accaaaggtc agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag
    50941 caccacaata atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg
    51001 acccttttta atctgctgtt tttcaaactg attaatcagc aaaaatagct taatcgtcag
    51061 aataaccgca atcagggacg tacagataaa atcgataacg cctggagtga taaatttgag
    51121 tttatagttt tctataacat aattaataat gctaccaaca gcactgataa ttatggtgta
    51181 gattaaaaat tgcaccgcat ggaataaaaa gccttttctt ttacgatttc cgcggcgaaa
    51241 ccaaaagctc atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc
    51301 attatttgta aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc
    51361 atgccggaaa cccctggcgg ggctatttta ccgcgacaat tcattcagat catcaatagt
    51421 cagggaagga agtagcaaca ttagctaact aatcaatgcg ttgattgtaa atccagctaa
    51481 gaggtgaggt tttcagagca gacaatggtg aaatgtcatg gtattgttac gtttaggtaa
    51541 caagaaattt gtctgcacaa ggattacatc atgattatgg cgaaactgaa gtcagcgaaa
    51601 gggaagaaat ttctctttgg tttgttggcg gttttcatta ttgcggcgtc ggttgtgact
    51661 cgcgcgacta tcggcggcgt tatagaacag tacaatattc cgctgtcgga gtggacgaca
    51721 tcaatgtatg tgattcagtc atcgatgatt tttgtttata gcctggtctt tactgtgttg
    51781 ctggcaatcc cgttgggaat ttatttcctt ggcggcgaag agcagtaagt aaaaaatagg
    51841 cccgataact cgggccttgt cagttattga ggagtcgtta atcgtcttct tcgtcatcca
    51901 gttcaacggg tgtctgatac tggtcaggtt taatgaccaa cagatcgcag cgaagatgat
    51961 caatcacctg ttccgccgtg ttgccgagga atgctgctga aataccggtg cgtcctaccg
    52021 tgcccagaac cacaatcccc gcctgtaagt gctccgccaa atcaggaatc acctcttctg
    52081 gcagaccttt ttctacgtgc gtcatgtttt cattaatgcc gaatttctgc cgcagggctt
    52141 tcattgccag caaatgttgc ccacgaatgg catcgttata aacgctcgga tcaaattccg
    52201 gcagttcaat cgcgatatta attggcgtta ccggataagc gccaaccaga tgaacttcgg
    52261 tatggttgac ttgttctgcc agttcgatcg tctctttgac cagtttttca ttgagcgcat
    52321 tatgatacgg ctcttcactg gcgagattca ccgccaccag cgccttacct ccttccggcc
    52381 acggctggtc tttcaccatc cacaccgggc ttgggcattt gcgtaacagg tgccagtccg
    52441 ttggcgtaaa aatcaccgct tccagacggt catgttggtg cgccattttt agcaccaaat
    52501 cgtgcccgcc gctgatcact tcctgaatga tggcttcgaa aggacggtta tgccagacca
    52561 ctttaatttc aatgggaacg ccagcattga gataatattt tgcctgctcg tggatccagg
    52621 ctgtacgctg gctgatgacg ccctgacgca tagcggtacg ttcgtccggg gagagcaggg
    52681 tggtcatttc gtatgagaag tcatagatcg gcaaaaaggc tttaattttg ccaccaatcc
    52741 gttgatgtaa ataaacagct cgccgcaatg ctggttggtc gtcctggtta ggatcgataa
    52801 caacgagcat gttctgatac atagccatac agggtctcct tacaacaact gtcaacgcag
    52861 tttgtaatta aaagattaac ccatatctgg tgaatgaaac agtgatgaac cttctgccag
    52921 atcaataaat cagaaaaatt taatgatatg acagaaggat agtgagttat gcggaaaaat
    52981 caggcaacgt tacgcgtatg accagcaagc tgggccagcg catcgttatt ttcgatggtg
    53041 atgtatttac ctttgactgc cagcataccg cttttctgga agcgacccag cagacggctg
    53101 atggtttcta ccgtcaggcc cagatagtta ccgatatcgc cacgagtcat cgtcaggcgg
    53161 aattcacgag gggagaagcc gcgttgggca aaacgacggg acaggttgta gatgaatgca
    53221 gccagacgtt cctcggcatt tttctttgac aacagcagga tcatgtcctg atcgcctttg
    53281 atttcaccgc tcatcagacg catcatctgc tgacgcagat tcggcatttt accggacaaa
    53341 tcgtccagcg tttcgaacgg gatttcacat accatcgagg tttccagcgc ctgcgcgaag
    53401 ctcgggtgat ggccgctgcc gatggcgtca aatcccacca ggtcgcctgc taaatggaaa
    53461 ccagtgattt gctcgtcgcc ttgctcagtg atggtataac ttttaatcgt accggagcgg
    53521 atggcataaa gcgatttaag ttcatcacca gccttaaaca gcgtctggcc tttctgaata
    53581 ggcttcttcc gctcaatgat attatcaagc tgatcaagct catgttcgtt gagtgtgaac
    53641 gggatgcaaa gctggctgat gctgcaatcc tggcaatgga tagcacaacc gccagactga
    53701 atgcgccgta taattcgctt ttccgggatc ataggtctgc tcaagccgta attgatattt
    53761 gtcaatttta acatcttttt agggagcaag taagtctaag caaaccttaa cagcag
//
LOCUS       DAZGXS010000017        51496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGXS010000017 DAZGXS010000000
VERSION     DAZGXS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..885)
                     /gene="rhtA"
                     /locus_tag="ACHKG5_001150"
     CDS             complement(<1..885)
                     /gene="rhtA"
                     /locus_tag="ACHKG5_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0867292.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1120..1185
                     /locus_tag="ACHKG5_001151"
     CDS             1120..1185
                     /locus_tag="ACHKG5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867293.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            1238..1753
                     /gene="ompX"
                     /locus_tag="ACHKG5_001152"
     CDS             1238..1753
                     /gene="ompX"
                     /locus_tag="ACHKG5_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0867294.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(1802..3385)
                     /gene="opgE"
                     /locus_tag="ACHKG5_001153"
     CDS             complement(1802..3385)
                     /gene="opgE"
                     /locus_tag="ACHKG5_001153"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0867295.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(3657..3785)
                     /gene="mntS"
                     /locus_tag="ACHKG5_001154"
     CDS             complement(3657..3785)
                     /gene="mntS"
                     /locus_tag="ACHKG5_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0867296.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            3971..4438
                     /gene="mntR"
                     /locus_tag="ACHKG5_001155"
     CDS             3971..4438
                     /gene="mntR"
                     /locus_tag="ACHKG5_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0867297.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4435..5553
                     /gene="ybiR"
                     /locus_tag="ACHKG5_001156"
     CDS             4435..5553
                     /gene="ybiR"
                     /locus_tag="ACHKG5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0867298.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(5611..6531)
                     /gene="ldtB"
                     /locus_tag="ACHKG5_001157"
     CDS             complement(5611..6531)
                     /gene="ldtB"
                     /locus_tag="ACHKG5_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0867299.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            6750..8342
                     /gene="ybiT"
                     /locus_tag="ACHKG5_001158"
     CDS             6750..8342
                     /gene="ybiT"
                     /locus_tag="ACHKG5_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0867300.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(8510..9775)
                     /gene="ybiU"
                     /locus_tag="ACHKG5_001159"
     CDS             complement(8510..9775)
                     /gene="ybiU"
                     /locus_tag="ACHKG5_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0867301.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(9927..10742)
                     /gene="ybiV"
                     /locus_tag="ACHKG5_001160"
     CDS             complement(9927..10742)
                     /gene="ybiV"
                     /locus_tag="ACHKG5_001160"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0867302.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(10888..13319)
                     /gene="ybiW"
                     /locus_tag="ACHKG5_001161"
                     /pseudo
     CDS             complement(10888..13319)
                     /gene="ybiW"
                     /locus_tag="ACHKG5_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(13325..14224)
                     /gene="ybiY"
                     /locus_tag="ACHKG5_001162"
     CDS             complement(13325..14224)
                     /gene="ybiY"
                     /locus_tag="ACHKG5_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0867303.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            14355..15017
                     /gene="fsa"
                     /locus_tag="ACHKG5_001163"
     CDS             14355..15017
                     /gene="fsa"
                     /locus_tag="ACHKG5_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0867304.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(15196..15945)
                     /gene="moeB"
                     /locus_tag="ACHKG5_001164"
     CDS             complement(15196..15945)
                     /gene="moeB"
                     /locus_tag="ACHKG5_001164"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0867305.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(15945..17180)
                     /gene="moeA"
                     /locus_tag="ACHKG5_001165"
     CDS             complement(15945..17180)
                     /gene="moeA"
                     /locus_tag="ACHKG5_001165"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0867306.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            17384..18349
                     /gene="iaaA"
                     /locus_tag="ACHKG5_001166"
                     /pseudo
     CDS             17384..18349
                     /gene="iaaA"
                     /locus_tag="ACHKG5_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            18336..20207
                     /gene="gsiA"
                     /locus_tag="ACHKG5_001167"
     CDS             18336..20207
                     /gene="gsiA"
                     /locus_tag="ACHKG5_001167"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP0867307.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            20227..21765
                     /gene="gsiB"
                     /locus_tag="ACHKG5_001168"
     CDS             20227..21765
                     /gene="gsiB"
                     /locus_tag="ACHKG5_001168"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0867308.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            21783..22703
                     /gene="gsiC"
                     /locus_tag="ACHKG5_001169"
     CDS             21783..22703
                     /gene="gsiC"
                     /locus_tag="ACHKG5_001169"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0867309.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            22706..23617
                     /gene="gsiD"
                     /locus_tag="ACHKG5_001170"
     CDS             22706..23617
                     /gene="gsiD"
                     /locus_tag="ACHKG5_001170"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0867310.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            23795..26143
                     /gene="pdeI"
                     /locus_tag="ACHKG5_001171"
     CDS             23795..26143
                     /gene="pdeI"
                     /locus_tag="ACHKG5_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0867311.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            26151..27479
                     /gene="dgcI"
                     /locus_tag="ACHKG5_001172"
     CDS             26151..27479
                     /gene="dgcI"
                     /locus_tag="ACHKG5_001172"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0867312.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(27549..27878)
                     /locus_tag="ACHKG5_001173"
     CDS             complement(27549..27878)
                     /locus_tag="ACHKG5_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0867313.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(27868..28254)
                     /locus_tag="ACHKG5_001174"
     CDS             complement(27868..28254)
                     /locus_tag="ACHKG5_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0867314.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(28480..29805)
                     /gene="rimO"
                     /locus_tag="ACHKG5_001175"
     CDS             complement(28480..29805)
                     /gene="rimO"
                     /locus_tag="ACHKG5_001175"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0867315.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            30018..30401
                     /gene="bssR"
                     /locus_tag="ACHKG5_001176"
     CDS             30018..30401
                     /gene="bssR"
                     /locus_tag="ACHKG5_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0867316.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            30512..31627
                     /gene="yliI"
                     /locus_tag="ACHKG5_001177"
     CDS             30512..31627
                     /gene="yliI"
                     /locus_tag="ACHKG5_001177"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0867317.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(31624..32250)
                     /gene="gstB"
                     /locus_tag="ACHKG5_001178"
     CDS             complement(31624..32250)
                     /gene="gstB"
                     /locus_tag="ACHKG5_001178"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0867318.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            32496..33698
                     /gene="dacC"
                     /locus_tag="ACHKG5_001179"
     CDS             32496..33698
                     /gene="dacC"
                     /locus_tag="ACHKG5_001179"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0867319.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(33745..34503)
                     /gene="deoR"
                     /locus_tag="ACHKG5_001180"
     CDS             complement(33745..34503)
                     /gene="deoR"
                     /locus_tag="ACHKG5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0867320.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(34561..35157)
                     /gene="ybjG"
                     /locus_tag="ACHKG5_001181"
     CDS             complement(34561..35157)
                     /gene="ybjG"
                     /locus_tag="ACHKG5_001181"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0867321.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            35442..36679
                     /gene="mdfA"
                     /locus_tag="ACHKG5_001182"
                     /pseudo
     CDS             35442..36679
                     /gene="mdfA"
                     /locus_tag="ACHKG5_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(36720..37004)
                     /gene="ybjH"
                     /locus_tag="ACHKG5_001183"
     CDS             complement(36720..37004)
                     /gene="ybjH"
                     /locus_tag="ACHKG5_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0867322.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(37090..37905)
                     /gene="ybjI"
                     /locus_tag="ACHKG5_001184"
     CDS             complement(37090..37905)
                     /gene="ybjI"
                     /locus_tag="ACHKG5_001184"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0867323.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(37905..39113)
                     /gene="ybjJ"
                     /locus_tag="ACHKG5_001185"
     CDS             complement(37905..39113)
                     /gene="ybjJ"
                     /locus_tag="ACHKG5_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867324.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            39197..39733
                     /gene="rcdA"
                     /locus_tag="ACHKG5_001186"
     CDS             39197..39733
                     /gene="rcdA"
                     /locus_tag="ACHKG5_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0867325.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(39908..41593)
                     /gene="ybjL"
                     /locus_tag="ACHKG5_001187"
     CDS             complement(39908..41593)
                     /gene="ybjL"
                     /locus_tag="ACHKG5_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0867326.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            41863..42240
                     /gene="ybjM"
                     /locus_tag="ACHKG5_001188"
     CDS             41863..42240
                     /gene="ybjM"
                     /locus_tag="ACHKG5_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0867327.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(42270..42527)
                     /gene="grxA"
                     /locus_tag="ACHKG5_001189"
     CDS             complement(42270..42527)
                     /gene="grxA"
                     /locus_tag="ACHKG5_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0867328.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            42687..42974
                     /gene="ybjC"
                     /locus_tag="ACHKG5_001190"
     CDS             42687..42974
                     /gene="ybjC"
                     /locus_tag="ACHKG5_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0867329.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            42958..43680
                     /gene="nfsA"
                     /locus_tag="ACHKG5_001191"
     CDS             42958..43680
                     /gene="nfsA"
                     /locus_tag="ACHKG5_001191"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0867330.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            43741..44643
                     /gene="rimK"
                     /locus_tag="ACHKG5_001192"
     CDS             43741..44643
                     /gene="rimK"
                     /locus_tag="ACHKG5_001192"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0867331.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            44731..45207
                     /gene="ybjN"
                     /locus_tag="ACHKG5_001193"
     CDS             44731..45207
                     /gene="ybjN"
                     /locus_tag="ACHKG5_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0867332.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            45558..46670
                     /gene="potF"
                     /locus_tag="ACHKG5_001194"
     CDS             45558..46670
                     /gene="potF"
                     /locus_tag="ACHKG5_001194"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0867333.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            46765..47898
                     /gene="potG"
                     /locus_tag="ACHKG5_001195"
     CDS             46765..47898
                     /gene="potG"
                     /locus_tag="ACHKG5_001195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0867334.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            47908..48861
                     /gene="potH"
                     /locus_tag="ACHKG5_001196"
     CDS             47908..48861
                     /gene="potH"
                     /locus_tag="ACHKG5_001196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP0867335.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            48858..49703
                     /gene="potI"
                     /locus_tag="ACHKG5_001197"
     CDS             48858..49703
                     /gene="potI"
                     /locus_tag="ACHKG5_001197"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0867336.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            49763..50251
                     /gene="ybjO"
                     /locus_tag="ACHKG5_001198"
     CDS             49763..50251
                     /gene="ybjO"
                     /locus_tag="ACHKG5_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP0867337.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            50292..51419
                     /gene="rlmC"
                     /locus_tag="ACHKG5_001199"
     CDS             50292..51419
                     /gene="rlmC"
                     /locus_tag="ACHKG5_001199"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0867338.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
BASE COUNT        12416 a        12551 c        13613 g        12916 t
ORIGIN      
        1 attaatgtct aattctttta ttttgctctc tttgcgtact gtcagcgtag aacccattga
       61 agcggcgata attgcgccga gcgccagcag ctgtatgggt gtcagtgttt ctccgaggaa
      121 aatcatcccg gaaacggcag ccagcgccgg ttccatgctc atcagcgtac caaatgtccg
      181 tgttggcaaa cgggtgaggg caatcatttc cagcgaataa ggcagagcgg tcgagagaat
      241 agcgaccgcc agacccaatg gaataaccga ccagtgccag agtgcttcac cagcctgaag
      301 cgctccaatt ggcacgaaaa ttaacgctgc aatcaacgaa ccaattgcca ccgtcgcagg
      361 gccatgttcc gctcctgcgc gttgcccact taaaatgtaa atagcccaac aagccccggc
      421 ccccagtgcc agcgcacagc cggttaaatc gacatgggaa acgtcttgcc ccagcggtag
      481 caggaaccac agaccaagaa ccgccagcac aacccagacg aaatctaccg ggcgacgaga
      541 agagaacagc gccaccgcca gtggtccggt gaactccagc gccaccgcaa tacccagcgg
      601 taccgtctga atagagagat aaaaaagata attcatccca cccagcgaaa cgccgtaaaa
      661 caacagcggt aaccgttgct ctttggcaaa gcgcagtcgc catggcttaa agaacgcgat
      721 gaggatcagc gttcctaatg ccaggcgcag cgcagtgaca cccggtgcgc ccaccagagg
      781 aaaaagtgac ttagctaacg aagctccacc ttgaatagac gccatggcaa cgagcaatat
      841 gactattggt aaccagaccg gcattttacg taatgaacca ggcatccttt ctcccacaaa
      901 tatctagact taagtaaagc gtggagtgta ctggatatac ccaatgctgg ttgagcattt
      961 gttgaaaaaa ttttcccccg ttttgactaa aatgcgccag gattgatgga atcattagtc
     1021 tggtgattag gaataatctg gatgaatgac agggaaaaca tgcgtaatac ttacgcagtt
     1081 ctctgaaaaa gtgatttaaa tttagatgga tagcggtgta tggaaacgtt ctgttacatg
     1141 aaatggcccg ttagacatca caaatcgcga agagtttccc attaattttt gatatattta
     1201 aaacttagga cttatttgaa tcacatttga ggtggttatg aaaaaaattg catgtctttc
     1261 agcactggcc gcagttctgg ctttcaccgc aggtacttcc gtagctgcga cttctaccgt
     1321 aactggcggt tacgcacaga gcgacgctca gggccaaatg aacaaaatgg gcggtttcaa
     1381 cctgaaatac cgctatgaag aagacaacag cccgctgggt gtgatcggtt cattcactta
     1441 caccgagaaa agccgtactg caagctctgg tgactacaac aaaaaccagt actacggcat
     1501 cactgctggt ccggcttacc gcattaacga ctgggcaagc atctacggtg tagtgggtgt
     1561 gggttatggt aaattccaga ccactgaata cccgacctac aaacacgaca ccagcgacta
     1621 cggtttctcc tacggtgctg gtctgcagtt caacccgatg gaaaacgttg ctctggactt
     1681 ctcttacgag cagagccgta ttcgtagcgt tgacgtaggc acctggattg ccggtgtagg
     1741 ttaccgcttc taatcacttt ggtgatataa aaaatccgcc tctcggggcg gatttttgtt
     1801 tttaaggctt cgggtcgaaa atatcggttc cgagatggtt gtaatccacc ttctgtaact
     1861 ggaagttggt gatgtatatc ggcccggctt tcttctcaga tataaacggg tatttgatgt
     1921 taatttcctt cgctttaatt cccgtccact gcgagaaaaa gcctaagaag tcattggctg
     1981 agcggcgggc tttaattacc cgatgcgctt tatcgtcgct ggaaatgacc ataaaaggca
     2041 cctggaaatt ttgctgatac ttatcatcat gggcaaggta ttgcacgtct ttgccgcgct
     2101 ctttaaaggc cagaccgtgg tcagaaaagt aaaccagcga gaagctgctg ccgctgttgc
     2161 gtaactgatc gtacagcttg cgcagtaaat cgtccgtttg cgtcatagta tagagatagc
     2221 acgacgtttc tttcgattgc acaaaggttt cgtatttgcc ttttgtccgg tcgcaggcct
     2281 gcggatgcga gcccatcaga tgcagaacaa tcagctgcgg ttgcgagtgc tcttgcgcca
     2341 gcacttgagc ggtcatatcc agtaacgctt cgtctttcgt gtttttatct gcttcaaaat
     2401 taccttcttt caggaagtac acttcatctg cacgtttggc gatgctggcg atagcagtat
     2461 cgtattcgcc gatttggccc tggttggaaa accaccaggt ctggaagccc gcgcgatttg
     2521 ccagggtgac aaagttatcc tgaaactgtg gtttgccatc gactacgcga ttgagcgtta
     2581 agccaagcga tttctgcgtg gagccactgg cggcaatgta gtcagcaaat atcaaaccgt
     2641 taacgctgct ggcaaacggg gtattgtccc agtgaccgcc aaaggcaccg agggcatcgc
     2701 gacgcgcgct ttcaccgatc accaccacat aggtctgata cttcggctta acggcagtga
     2761 ccgtccaggt atctttcatt ccggaaagtt ttgccatccg ttcttgctct tcaatcacct
     2821 cgttattatt gacgatgaca tccttagcaa aacgaaatac cggatagcca gtatctttca
     2881 gtttaaatac gccaccccag gccaggtttt gcacgggagc aacaaaaaat gtcgccacac
     2941 tgaataccag acacaggctg tcgaaggtat tccagcgcgc tttttcattc tctttttttc
     3001 gcctgattgc tattacgccg agtgcaaaaa taaataagcc gaccaggtag ctgtaccacg
     3061 gaaaaatcgt caggatttcg gtcgactctt ccatattggt ggagtgcaat gccagcaatg
     3121 tattgaaatt aggcgcgccg taggcctgag caaaagggaa ataacaagcc gccaccagcg
     3181 aactgacacc gaccagaact ttttgtacgc gaggcaatgt tcgccatagc aaaagcaaaa
     3241 tagccgtaaa cgcagcggtg tagagcaaac tgaaggggta acctaagccg aggttaatta
     3301 atagcgactg taaaaagtag aacgcagtcc acgggctaaa tacccggcta cgggtaacaa
     3361 gcgattcttt gagggttaaa ttcatatgcc actatcgtaa caaacgccat gtgcttaccc
     3421 tggcgtcaag ggtcaatacc tgcaagagtg cgtgaagagg ggaaaggggt ccgcatccgc
     3481 gagccgcaat atacgcaggg ctgcaagaag atagagcgag ccgcagttca ggtcaactac
     3541 tgacgaacaa atcttttgcg aaggggattg caaatccgac aaaaagtggg gtttttcaga
     3601 ccaaaacgcg tcgtgcctgc atgaaagcag gagtgaagcg gcgtaccgcg acgccgctat
     3661 ttatcggaag gtttatcttg ctgcggtttg ttgttgacca tatcgcacaa catagagagc
     3721 agcattaacc gtactttaaa gggagaatga ctaaacacgc gcatacacct cttgaactca
     3781 ttcataagac ctcctgactt gctaatcccg tcgatccttg agggatgatt gcattacata
     3841 cagatatagc acaggctata ttatatagct attgctaaaa cgttaatttt ttgtaccttc
     3901 gcaactctgg tttacaatgt gcgcacgaaa tgagaacgct atgtatgcgt caccataatg
     3961 aggaagcaca atgagtcgtc gcgcaggtac gccaacagca aaaaaagtga cgcagttagt
     4021 gaacgaggaa gagcacgttg aagggttccg ccaggtcaga gaggcgcatc ggcgcgagct
     4081 tattgatgat tacgttgagc tgatttctga cttgatcagg gaagtggggg aagctcgtca
     4141 ggtagacatg gctgctcgtt tgggagtttc gcaaccgacg gtggctaaaa tgcttaagcg
     4201 actggcaact atggggctga ttgaaatgat cccctggcgt ggcgtgtttt taacggcaga
     4261 aggagagaag ctggcgcagg aaagccgcga gcgacatcag atagtcgaaa atttcttgtt
     4321 ggtgttgggc gtcagtccgg aaatcgcccg tcgcgacgcg gaaggcatgg agcaccatgt
     4381 tagtgaagag acgctggatg cctttcgttt gtttacccag aaacacggtg ccaaatgagc
     4441 ctgccttttt tacgcacgct gcaaggcgat cgtttttttc agttattaat tcttgttggt
     4501 atcggattaa gttttttcgt gccctttgca ccgaaatcct ggcctgctgc tatcgactgg
     4561 cacaccatca tcaccttaag cggcctgatg ctgctgacca aaggtgtgga gttaagcggt
     4621 tattttgatg tgctggggcg caaaatggtg cgccgctttg ctacggagcg tcggctggcg
     4681 atgtttatgg tgctggcggc ggcgctgctt tctacctttc tgaccaacga tgtcacgctg
     4741 tttattgttg ttccgctgac tatcacgcta aaaaggctgt gtgagatccc ggttaatcgg
     4801 ctgattattt ttgaggcgct ggcagtcaac gctggttcgc tactgacgcc aattggcaac
     4861 ccgcaaaata ttcttatctg gggacgttct ggtctttcgt ttgccggatt tattgcccaa
     4921 atggcaccgc tggctggcgc aatgatgctg acgctcctgc tgttgtgctg gtgttgtttc
     4981 cctggaaagg cactccaata ccatacgggg gggcaaacac cggagtggaa accgcggctg
     5041 gtgtggagtt gtctggggct gtatatcgtc tttctgacgg cgctggagtt caaacaagag
     5101 ctgtggggac tggtgattgt ggcggcgggc tttgcgctgc tggcgcgtcg cgtggtgttg
     5161 agtgtggact ggacgctgct gctggtgttt atggcgatgt ttatcgacgt ccatttactg
     5221 acccagcttc cagcgttgca aggcgtgttg ggtaacgtga gtcatttatc tgaacccgga
     5281 ttatggttaa cggcaatcgg tttatcgcag gtgatcagta acgtgccgag tactatattg
     5341 ttgctgaact atgtgccgcc gtctttatta ctggcatggg cggtaaacgt aggtggcttt
     5401 gggttattac ccggatcgct ggcaaatttg attgcgctac gtatggcgaa cgatcgccgc
     5461 atctggtggc gtttccatct ctattcaata ccgatgctgt tgtgggcggc gctggtggga
     5521 tatgttttgt tggttatgat cccggcctgg taaggtctgg caaataaaaa aggcggatta
     5581 tgagtccgcc tttgttttac gttttgaaga ttaattcaga cgaaccggca tcccggagcg
     5641 gtttttaatc gcttcatcaa gaacaacctg gtcaacatct ggctggccgg tcactgtctg
     5701 cacgctcttc gtcagggtaa ttggcacaat ttcctgacct tcaaattggg cttcggtggt
     5761 agacagcggg ttatggactt caatgtaacg gctgccgtct ggctcggtgg tcgcttttac
     5821 cggctcatca ataaactgta cgcgggtacc gaccggtact ttctcgaaca ggaatttgat
     5881 gtcttcgtta cgcagacgca cacaaccatg acttacacgc agaccgatac cgaagttagc
     5941 gttggtgcca tggatagcgt acaggcgacc gatgtagagt gcatacagcc ccatcgggtt
     6001 atccggacct gccggaacga cagccggaag cggttcgccc gcagcgcggt actctgcgtg
     6061 cattttggcg gtcggcgtcc aggtcgggcc tgctttctta cgctcaactt tggtggtcca
     6121 gttgataggc gtatctttgc ctaactgacc aatgccgatc ggcagcacga taacggtgtt
     6181 ggtccctttc ggatagtaat acagacgcat ctctgcactg ttaatgacga tgccttcatg
     6241 aacggtatcc ggcaggatca gctgctgcgg aatgttcagt acagtaccgc ctttcggcag
     6301 gaaggtatcc acacccgggt tcgcttccat catattggaa agccccatct ggtactccgc
     6361 ggcaaaatac tccagcggct gagtgttacc ttcaggaatg gtgatcacct gattctgacc
     6421 aaccaggcga ctcccgtcgg ttggcagagg ataagttacc gcagaggcgg tactgcaaaa
     6481 gccgacaaca gcgaaggccg ctgcgaataa tgttttcaat ttcatattca tgttaagcga
     6541 gattttgtgc ctggcaggcc attgggttga gaatattaga gtattggaag cgcattataa
     6601 gttcattcca gctcacagtg aaatcagatg tgtacgaaat cacatttttt gcctttggct
     6661 tgagtgtaga tcttaagcga ggagcaggat cttctttcag acttatggca taatgcgcgg
     6721 tttgtcatat ctcttttcag gatacgcctg tgttagtttc cagtaacgtc accatgcagt
     6781 tcggcagtaa gccgttgttt gaaaacattt ccgtcaaatt tggcggcggc aaccgttacg
     6841 gcctgattgg cgcgaacggt agtggtaaat ccacctttat gaagatcctc ggcggcgacc
     6901 ttgagccgac gctgggtaac gtttccctcg atcccaacga gcgcattggt aaactgcgtc
     6961 aggatcagtt tgcctttgaa gagttcactg tgctggatac ggtgatcatg gggcataaag
     7021 agttgtggga agtgaagcag gagcgcgacc gcatctatgc tttgccggaa atgagtgaag
     7081 aagacggcta taaagtggcc gatctggaag ttaaatacgg cgaaatggac ggttactctg
     7141 cggaagctcg tgccggtgaa ttgttgcttg gcgtgggaat tccagtggaa cagcactacg
     7201 gcccgatgag tgaagttgct cctggctgga agctgcgtgt gttgctggcg caggcgctgt
     7261 ttgctgatcc ggatattctc ctgctcgacg aaccgaccaa caacctcgac atcgacacca
     7321 ttcgctggct ggaacaggtg ctgaacgagc gtgacagcac catgatcatc atctcgcacg
     7381 accgtcactt ccttaacatg gtctgtaccc acatggcgga tctggattac ggcgagctgc
     7441 gcgtttatcc gggtaactac gatgagtaca tgacggcggc gacccaggcg cgtgaacgtc
     7501 tgctggccga taacgccaag aagaaagcgc agattgctga gctgcaatct ttcgttagcc
     7561 gctttagcgc caacgcctcg aaatctcgcc aggcaacttc gcgcgcgcgc cagattgata
     7621 aaatcaaact ggaagaggtg aaagcctcca gccgtcagaa cccgttcatc cgttttgaac
     7681 aggataagaa gctgttccgt aacgcgctgg aagtggaagg tctgaccaaa gggtttgata
     7741 acggtccgct gtttaaaaat ctcaacctgc tgctggaagt cggtgaaaaa ctggcggtac
     7801 tgggtaccaa cggcgtcggt aaatcaacgc tgttgaaaac gctggtgggc gatctgcaac
     7861 cggacagcgg caccgtaaaa tggtctgaaa acgcgcgcat tggttactat gctcaggacc
     7921 acgaatatga gtttgaaaat gatctgaccg tgttcgaatg gatgagccag tggaagcagg
     7981 aaggcgatga cgagcaggcg gtgcgcagta ttctcggtcg tttgctgttc agccaggacg
     8041 acatcaaaaa gcctgctaaa gtgctttccg gtggagaaaa agggcggatg ctttttggta
     8101 agttaatgat gcagaagccg aacattctga tcatggacga accgaccaac cacctggata
     8161 tggaatccat cgagtcactg aacatggcgc tggaactgta tcagggcacg ctgatctttg
     8221 tttcacacga ccgtgagttc gtaagctccc tggcgacccg cattctggaa atcaccccgg
     8281 aacgcgtgat cgactttagc ggtaattacg aagattacct gcgtagtaaa gggatcgagt
     8341 aagttattat ccgtcacccc gatactgtcg gggtgacggc ggctgatgac aaacgcaaga
     8401 ttgcctgatg cgctatgctt atcaggcatt aagaaagcgc acttgtcagc aatctgagta
     8461 acgtcgaatc gattttctta atcccacatc aattcacttc aattaactct caaacatcca
     8521 tgcccaacgc tcgcttaccg tgaatgttga ggtcggcaag cgtaaagcgt ccttcccagt
     8581 ttgtttcata gtcctcgcgc gggaagtcgc ccggcgatgc gcctttttcc agtgcggcct
     8641 tcaccttgtg cgcgtaggca agatttttct cgcacatcgg tgccgcagga atgtacatca
     8701 cgttgcccca accctgttga ttttcaacgg gggcaacgga atggatgacg tcgcagtgcc
     8761 accagacgga gtctccggcc tcgagttttg gaatgctggt taacgcctca attaacagtg
     8821 gatgccattg ctctgatacc ggcaacactc ttccgggcgc tacgccgcac agttcatcct
     8881 ccggcacatc atcaagcagc ggacgtaaca gtacgtacgc catggcttca ggaatgggca
     8941 cgacgtgcag caacccctga ctaggcagca tatcagagag cgctgtccag ccctggaatg
     9001 tccgaaacac ggaacatttg gtggtgttgt ccaccgtgta ctcttcaact tccgtacgat
     9061 gtgccgcatg ccagggatcg tatttcgcca gattgccatt aaagacgtta gcgaaaacgt
     9121 gctgatacgc tggaagcagc cagcgttcca gcgccccgga gtcggtatgc gctccaagac
     9181 ctttggaggt cgttccgggc ggacggcggc ggatacggtc agggtagatg acgctcacat
     9241 ccgggttaaa ccattgcttt ccatcacttt caaatgtcca cagacgattg agaaacgact
     9301 gcgcattcgc catttcttca ctctggcggg cctgcatttg cgcctgcgac cagtagatgg
     9361 ggtaaatctc gggacgtgaa gcgctgagcg tcccgaagaa attatcgccg gggcctttgt
     9421 agacctcgtc aaagcggttg cggtccagat agtccagcat cgactgatcc cagcctagcg
     9481 cttgttcgcg ggggaaatgg ccttttatca ccgcacaacc gcgacgttta atctgttcgc
     9541 gctgctctgc agtaacatga cctgctttga tatcggcata agacagcacc ggccagacgg
     9601 catcgccctg tgctttgagt gcgttgattt cagccactcg cgtggcaatg tcatcgctta
     9661 gctgattaaa gatctgctgg acgtcgccaa gctgcgcccg cagcgcgtgc ttcatctgac
     9721 ggatagctgc tttgtgatcg gcaggcaatg tgtcgctggt aaaagtagaa gccataacca
     9781 cctcgcatta actttcattc gaaactatgc tgatttgcat gtgacaatat aagttataaa
     9841 taagttaatg caagtttaat gatttgatgt gatgtacagg atgtaaaaag aagggataac
     9901 cggagcgcat ggctccggta gagaggtcag ctgttaaaag gggatgtatt atccagtaca
     9961 gcctgaatca cattcagcgc gccttcatga ttattatcat cggtagcgta acgggcgatt
    10021 tgtttaatgt tttccgcagc attgcccatc gcaaaggaat aacgcgccat tttcagcatc
    10081 tccgcatcgt taccgctgtc gccaatcgct accacatttt gcggtgacag atcccagcgt
    10141 ttcagtaacc gcgaaatacc gtttgcttta tgtagaccgg gaataatcag gtcgataaag
    10201 ccaaaaccac tggtgacggg tttcataatg ccatcgagcg ctatgtgcag tttgtcgatc
    10261 actaacggga tttgttcatc cggcaggttg agtgaaaact tgaacagtac gtcgtcaatc
    10321 tcctgataat cttttacagg tttcaggcga tggtagtgtt ttgccatcag tgcgacaaat
    10381 gcttcggggg cattttcgct gacatatgca ctttgcagac cgcaggcgac aaaattgagt
    10441 tgcttatctt ttagcaactc gccaataaca atccgcgatt catgtcgggt cagttcgccg
    10501 tggaacagtt gcttgccatg ttcgtaaacc agtgcgccgt tttccgcgac aaaagagatc
    10561 tcatccttta gctcaggaaa gaatgaaata agctggtaat actgattacc gctggcgaca
    10621 acgaactcaa tgccgcgctt tttcagttcc tgatattgcg ccataaaacg tggttggttg
    10681 tacgttttga cgtcgttaag aaaagtaccg tccatatctg tgacgataac ttttacgctc
    10741 ataggtgtgc tcccggctcg aaaatgaaac cgtaacagtg taataacaat gtgacgcaga
    10801 gcacaaatta tatttcgaat gaaagtaagg atgaaattga tgatgtgaat gatttagccc
    10861 ggcgacgtct ccgccgggct gaggggatta cagcatatgt tcggtacggg cgatgatatc
    10921 gtcctgagcg tctggagaga gcgcggtgaa gaaggcggaa taacccgcga cacgcactac
    10981 cagatcgcga tactgatcgg gatgtttttt cgcctccagc agcgtttcgc gggaaacgat
    11041 gttgtactga atatgccagc ctttatgcac ttcaaagaag gtgcgcagca ggatcatcag
    11101 tttctgcttg tcagattcgt tctccagtgt tgctggattc agtttctggt tgagcaacac
    11161 cccgccgaga atcgctgccg taggcagttt acccaccgag ccaatgaccg cagtagggcc
    11221 aagatgatca gtaccggaag ccgggcttgc gccttctgcc agcggggtgt gggctttacg
    11281 tccgtctggc gttgccatag tttgcgcgcc aaacggtacg ttagcggaga tagacgacgt
    11341 acccgcgtaa tagttgccgc caaccggacc acgaccgtag cgcgggttat ggtactgttt
    11401 cagttcgtcg atataggtct gataagcgcg agccagcagc gtatcgacag tatcatcgtc
    11461 gttgccgtac ttcggcgcac cgttaatcag ccgctgacgc agctgctcgt gagtcaggcc
    11521 gtcgaagtca ttcgccagtg cggcagccag ctgttgctga ctaatcgcgc cctgttcaaa
    11581 taccagtttc ttcacggccg ccaggctgtt gccgaggttg gcaatgccaa cctgcaggcc
    11641 agaaacccag tcatatttcg cgccgccttg cttgatactt ttcgctcgct caatacagtc
    11701 atccaccagc gccgagcaga gaatatcgtg cacgttctct tccagcatgg tgtcgacgac
    11761 atattcgatt tcgattgatt tacgcgtgta gtaacggatt tgcgtatccc acgcgtccat
    11821 cacttcatcg aagttgttga agttacctgc cgacaacgct ttttcttgtg gcaggaacac
    11881 tttgccgctg gtggcatcac gcccgccttc cagcgccgcc agcatcacgc gggcgaagtt
    11941 gataaagctc atgccggtac agcgatagcc ccatttgcca ccgacggcgg tttcgataca
    12001 accaatcgct gcatagttgt aagcatcctg cggttcaata ccgagtttaa taaattccgg
    12061 gatgacgatt tcgtcgttgt tgaacgccgg catcccgaag ccgcaacgga tcacctgtac
    12121 gcaggcgtcg aggaaatcgt tgctcattcc cgcgtggtaa cgcacgctga ggttaggctg
    12181 agtggaacgc aggcgaccgc aggattcgag gatcgcgtaa gagagtggat tcaccgcgtc
    12241 cattggttga ccatcaacca gattttgtcc gccaatggtg acgttctgat acagaggact
    12301 tcccgcagag gcttttgagt gtgagcctga gcggattttg ttcacttcca gcagtttcag
    12361 ccagcagcta tgcagcaatt cgatggcgtg ttcgcgatcc agtgtctggt tgagttcaac
    12421 gtcgcggcga tagtacgggt agagatactg gtccatacga ccaaacgata ctgagtgacc
    12481 gttagattcg atctgcaaaa tcaactggat gaagtaacac agttgcagcg cctgccagaa
    12541 agtctgcggc ggctggtggg cgataagatc gcagttttct gccatcgcca gcagttcatc
    12601 gcgacggctt tcgcgggttt cggtcgcggc cattgcacgc gccagggcag cgaaacgttc
    12661 aatgtgttca ctgactgcca ccagcacgat atcaatcgct ttcaggaact gttcgccgtg
    12721 cagatcttcc agtaccgtca gattgatgcg cgagcgacgt tccgctactt tctcgcgcag
    12781 accatcaagc ctttttccag cagcagcggg aaattcaccg ccaggtgcgc atcgccggag
    12841 gtcatattgc cttccgcttt aatgattccg gtcgccagca gacctttttg ctcatcggta
    12901 aacatgccgt agcagcgatc ctgtaccgtc tgaccgcgcc accacgggca cacttcatgc
    12961 agaacgcgtt tgttctcttc gctcaccgca aagccagcac cgggacgatc tgccagatca
    13021 tcaatctctt tttcgatcca cgagacagta tattccggga agatcggcgc ggcgcgaact
    13081 tcgcttgcct ggttgccgat gatcaactca tcgtgcttga tccagatagt gcgattcgcc
    13141 aggtgatgtg ccagcgccag cgcgcgacgt acagggatcg gcttatcgag atgttgttga
    13201 tacatctcgg tatagtgctg cgcgcgctcg gtacaaaccg gcggtttcac aatatgcacc
    13261 agcgcatttt tgtgcgcttt aatgcggtcg ctgagcgtgt ccagtttcag tgtggtcatg
    13321 gttgttatcc tcgtaaggtc gcggttaacc ctttctggca ggcatactgc tgggcaaagt
    13381 cgagcagttc tggcgcatca agcggttttt ccggggcgtc atagggcaga ttaagtaagt
    13441 gatatttgtt gatgcccagc gtgtggtagg gcaggaaatg aatttcgcca acgtgcagct
    13501 cgtcggcggc aaaatcggta atggctttca cagaggtttc gtcggcatta aagccttgaa
    13561 tcagcggcac gcggataatg atttttttgc ccgctgcggc gagttttttc aggttatcca
    13621 gcaccctggc ggcgttaccg tcggtccact gtttaaacgg cgcgtcggca acgtgtttta
    13681 aatcggcaag aaacagatcg atatagggca gagaaggggc gatatatttc cacggcacat
    13741 gcaggcaggt ttctaccgca gtatgaatgc cggcctcgtg gctggcttgc agtagcgccg
    13801 tcgccatttc cggctgcatg aatggttcac cgccagaaag tgttaagccg ccgccgctgc
    13861 gatcgtaaaa cggtttatcg cgcagaacgg tcaccatgat ctcctcaacg cttttcactt
    13921 ccccacacac ggttaacgac tgtgtcgggc agcagtcggt taacgccgtc agatgctccg
    13981 gggttaactt ttcccgatga ataagcaaac cattcagcgc gcgctcaatc acttccggcg
    14041 cggtcttagc gcacagctca cagccttcca ggcacagacg tgggtcatac agcagatcct
    14101 gcgtgcgggc gcggctttcc gggttctgac accagcggca gcccagcgaa cagcctttaa
    14161 gaaatacgac cgtgcggata ccggggccat catgggtcga gtagcgctga atattgaaaa
    14221 tcatagttgc ctctctgttt cgttcaaata ttaaaatact ttcgaatgaa agttagattg
    14281 atgtgcgtca actgttcaga gagttttccc gtgatagtct acattcagac aaaaagtaca
    14341 ttttgaggat ggttatggaa ctgtatctgg atacttcaga cgttgttgcg gtgaaggcgc
    14401 tgtcacgtat ttttccgctg gcgggtgtga ccactaaccc aagcattatc gccgcgggta
    14461 aaaaaccgct ggaggttgtg cttccggaac ttcatgaagc gatgggcggt caggggcgtc
    14521 tgtttgccca ggtaatggct accactgctg aagggatggt gaatgacgca cgtaaattgc
    14581 gttctattat tgcggatatc gtggtgaaag ttccggtgac caccgagggg ctggcagcta
    14641 ttaagatgtt aaaagcggaa gggattccga cgctgggaac cgcggtatat ggcgcagcac
    14701 aagggctgct gtcggcgctg gcaggtgcgg aatatgttgc gccttacgtt aatcgtattg
    14761 atgctcaggg cggtagcggt attcagactg tgaccgactt acaccagtta ttgaaaatgc
    14821 atgcgccgcg ggcgaaagtg ttagccgcga gtttcaaaac cccgcgtcag gcgctggact
    14881 gcttactggc aggatgtgaa tcaattactc tgccactgga tgtggcacaa cagatgatta
    14941 gctatccggc ggttgatgcc gctgtggcga agtttgagca ggactggcag ggagcgtttg
    15001 gcagaacgtc gatttaaccg gacgttctgc atcctcataa attgctgatg acgtggcgga
    15061 gtgcaccgat gcctgccgcg cttggaaatc tgtgcccgaa ccgtaggcca gataaggcgt
    15121 tcacgccgca tctggcgtct ggagcaaatg cctgatgcga cgcttgtcgc gtcttatcag
    15181 gcctggaggt ggcaattact gcccgcacac ctcacacccc ggattgcgca tcagtttcat
    15241 ttcgcgaaac tgacaggtca tcgcatcgta catgacgatt ttcccgctgg ccggtttgcc
    15301 ataacctgcc agcagtttga tcgcttccat tgcctgcaac gaaccaatca cgccgatcaa
    15361 cggtgccatc acgcctgctt ctacgcaggt taacgcgttc tcgctaaaca aacggctaag
    15421 gcagcgatag cacggttcgc cgtcctgata agtgaagacc gtgatttgcc cttccatacg
    15481 aattgccgcg ccggaaacca gcggcacctt cgcggcaaaa cagcctgcgt tcagttgatt
    15541 acgtaccgca acgttatccg tacagtcgag cacaagattg tgttcagcaa tcaatgctgc
    15601 aagttctgcg tcatccagca gtgcattgac tggcgtaatc gcgatatgtg ggttgatccg
    15661 cgtcagagcg tcacgggcgg attccacctt cggttgcccg accgtagcat cactgtgcag
    15721 cgtctggcgt tgcagattcg agagcgaaac cgagtcgaag tcgagcagcg tcaggttgcc
    15781 gacaccggcg cttgccagat actgcgaagc tgcgcagccg agtccaccga ggccgacgac
    15841 cagcacacga gaatctttca gcgcctcctg accgtcaaaa tcaaaaccgc gcagaatgat
    15901 ttgccggttg tagcgcagca tctcttgatc gctgagttcc gccattacag gcctccgaac
    15961 aacgcgttaa acggttctac ttccacccat tcgcccactt ccacattgcc gcgatcgcgt
    16021 tccagcacga taaagcagtt gccgaggcta aaggagctga atatatgtga accctgatgt
    16081 ccggtggtcg tcacttccag ttcgccatcg gcgttgcggt gcagcacgcc gcgctggaaa
    16141 tcaagacgtc ctggcgtttt tttcaggcgg gacgctgtgc gtacgcgctg gcgcgcgggc
    16201 aggccgctgg cggtgttgcc gcttagtttt gccagcaaag gctgtaccag ttgatagaag
    16261 gtcagcgtcg ctgaaaccgg gttgcccggc aggccgcaga accagctatt gctgagttta
    16321 ccgaacgcga acggtttacc tggtttaatc gccagcttcc agaaggcgat ctcccccagc
    16381 tcttcaagaa tcgttttggt gtaatccgct tcacccactg aaacaccgcc ggaactgatc
    16441 accacatccg cctggctgtc ggcttcaata aatgcggcgc gcagggcatg tggatcgtcg
    16501 cggataatcc ctaagttgat tacctcgcaa cccagctgtt ctaacatcag gtgtacggcg
    16561 agacggttgg tatcgtagat ttggccgtcg cccagcggct ggcctggcaa ctggagttca
    16621 tcaccggtag aaaaaagcgc tacacgcact ttacgaatca ccggaacttc ggcaatcccc
    16681 agtgaagcaa ttactggcag ctctgcggta gtcaggcgag tccccgccgg gaaaacaacc
    16741 gcacctgcag agatatcttc accgcgacgg cgaatatttt gcccgctacg cacttcagca
    16801 gtaaaacgca cgccattgtc cgtttgttca gtctgctcct gcatcaccac cgcttcgcag
    16861 ccttccggca ccggcgcacc ggtcataata cgaatgcagg tacccgcagg ccattcacca
    16921 tggtacggct gaccggcaaa ggatttaccg gcaacgggca gcggttgtcc ggaggtgata
    16981 tcggccaaac gtaccgcgta accatccatt gcggagttat caaaccctgg gacatccagc
    17041 ggcgaaacga catcgctcgc cagaatacga ccaaaacact gaaccagtgg cagcgtttcc
    17101 tgggcggtca gtggggtgac gcgagaaagc atctcattaa gcgcggtgtc gagcgacatc
    17161 aatccggtgg taaattccat gaaaacactc ctgcggaggc aaaatcgaat ttgcctatta
    17221 tgtcagaaaa acgccacaga ctgtatgcca cctcgggcgt agcgctgggt cctgccttta
    17281 catgccatat ccatctttct atattcaaaa attgaatgag taattcataa aaattctgat
    17341 atttatagca aaagtggcga gccaccctta atggacgaat actatgggca aagcagtcat
    17401 tgcaattcat ggtggcgcag gtgcaattag ccgcgcgcag atgagtctgc aacaggaatt
    17461 acgctacatc gaggcgttgt ctgccattgt tgaaaccggg cagaaaatgc tggaagcggg
    17521 cgaaagtgcg ctggatgtgg tgacggaagc ggtgcgtctg ctggaagagt gtccactgtt
    17581 taacgccgga attggcgccg tctttacgcg tgatgaaacg catgaactgg acgcctgtgt
    17641 gatggatggt aacaccctga aagccggtgc ggtggcgggc gttagtcatc tgcgtaatcc
    17701 ggttcttgcc gcccggctgg tgatggagta aagcccgcat gtgatgatga ttggcgaagg
    17761 ggcagaaaat tttgcgtttg ctcatggcat ggagcgcgtc tcaccggaga ttttctccac
    17821 gcctttgcgt tatgaacaac taatggcagc gcgcgaggaa ggggcaacag tcctcgacca
    17881 tagcggtgcg ccactggatg aaaaacagaa aatgggcacc gtgggggccg tggcgttgga
    17941 tttagacggc aatctggcgg cagccacgtc cacgggcgga atgaccaata aattacccgg
    18001 acgagttggc gatagcccct tagtgggtgc cggatgctac gccaataacg ccagtgtggc
    18061 ggtttcttgt accggcacgg gcgaagtctt catccgcgcg ctggcggcat atgacatcgc
    18121 cgcgttaatg gattacggcg gattaagtct cgcggaagcc tgcgagcggg tagtaatgga
    18181 aaaactccct gcgcttggcg gtagcggtgg cttaatcgct atcgaccatg aagggaatgt
    18241 cgcgctaccg tttaacaccg aaggaatgta tcgcgcctgg ggctacgcag gcgatacgcc
    18301 aaccaccggt atctaccgtg aaaaagggga caccgttgcc acacagtgat gaacttgatg
    18361 ccggtaatgt gctggcggtt gaaaatctta atattgcctt tatgcaggac cagcagaaaa
    18421 tagctgcggt ccgcaatctc tcttttagtc tgcaacgcgg tgagacgctg gcaattgttg
    18481 gcgaatccgg ctccggtaag tcagtgactg cgctggcatt gatgcgtctg ttggaacagg
    18541 cgggcggttt agtgcagtgc gataaaatgc tgttgcggcg gcgcagtcgt gatgtgattg
    18601 aacttagcga gcagagcgct gcacaaatgc gccatgtgcg cggtgcggat atggcgatga
    18661 tatttcagga gccgatgaca tcgctgaacc cagtatttac tgtgggtgaa cagattgccg
    18721 aatcaattcg tctgcatcag aacgccagtc gtgaagaagc gatggtcgag gcgaagcgga
    18781 tgctggatca ggtacgcatt ccggaggcac aaaccattct ttcacgttat ccgcatcaac
    18841 tctctggcgg gatgcgccag cgagtgatga ttgcgatggc gctgtcatgc cgcccggcag
    18901 tgctgatagc cgatgagcca accaccgcgc tggatgtcac tattcaggcg cagatcctgc
    18961 aattaatcaa agtattgcaa aaagagatgt cgatgggcgt tatctttatc actcacgata
    19021 tgggcgtggt ggcagagatt gccgatcggg tactggtgat gtatcagggc gaggcggtgg
    19081 aaacgggtac cgtcgaacag atttttcatg caccgcaaca tccctacacc cgtgcgctgt
    19141 tagctgctgt tccgcaactt ggtgcgatga aagggttaga ttatccccga cgtttcccat
    19201 tgatatcgct tgaacatcca gcgaaacagg ccccccccat cgagcagaaa acggtggtgg
    19261 atggcgaacc tgttttacgg gtgcgtaatc tggtcacccg tttccctttg cgcagcggtt
    19321 tgttgaatcg cgtaacgcgg gaagtgcatg ccgttgagaa agtcagtttt gatctctggc
    19381 ctggcgaaac gctatcgctg gtgggcgagt ctggcagcgg taaatccact accgggcggg
    19441 cgttgctgcg cctggtcgaa tcgcagggcg gcgaaattat ctttaacggt cagcgaatcg
    19501 ataccttgtc acccggcaaa cttcaggcat tgcgccgcga tattcagttt atttttcagg
    19561 acccttacgc ttcgctggac ccacgtcaga ccatcggtga ttcgattatc gaaccgctgc
    19621 gcgtacacgg tttattgcca ggtaaagaag cggttgcacg cgttgcgtgg ttgctggagc
    19681 gcgtgggcct gttacctgaa catgcctggc gttacccgca tgagttttcc ggcggtcagc
    19741 gccagcgcat ctgcattgct cgcgcgttgg cattgaatcc aaaagtgatc attgccgacg
    19801 aagccgtttc ggcgctggat gtttcaattc gcgggcagat tatcaacttg ttgctcgatc
    19861 tccagcgtga tttcggcatt gcgtatctgt ttatctccca cgatatggct gtggtagagc
    19921 ggattagtca tcgtgtggcg gtgatgtatc tcgggcaaat tgttgaaatt ggtccacggc
    19981 gcgcggtctt cgaaaacccg cagcatcctt atacgcgtaa attactggcg gcagttccgg
    20041 tcgctgaacc gtcccgacaa cgaccgcagc gtgtactgct gtcggacgat cttcccagca
    20101 atattcatct gcgtggcgaa gaggtggcag ccgtctcgtt gcaatgcgtc gggccggggc
    20161 attacgtcgc acaaccacaa tcagaatacg cattcatgcg tagataacat tcaggcggag
    20221 aataaaatgg caagagctgt acaccgtagt gggttagtgg cgctgggcat tgcgacagcg
    20281 ttgatggcat cttgtgcatt cgctgccaaa gatgtggtgg tggcggtagg atcgaatttc
    20341 accacgctcg atccgtatga cgcgaatgac acgttatctc aggccgtagc gaaatcgttt
    20401 taccaggggc tgttcggtct ggataaagag atgaaactga aaaacgtgct ggcggagagt
    20461 tataccgttt ccgatgacgg cattacttac accgtgaaat tgcgggaagg aattaaattc
    20521 caggatggca ccgatttcaa cgcagcggcg gtaaaagcga atctggaccg ggccagcgat
    20581 ccggcgaatc atcttaaacg ctataacctg tataagaata ttgctaaaac ggaagcgatc
    20641 gatccgacaa cggtaaagat taccctcaaa cagccgttct cagcgtttat taatattctt
    20701 gcccatccgg cgaccgcgat gatttcaccg gcagcgctgg aaaaatatgg caaggagatt
    20761 ggttttcatc cggtgggaac cggaccgtat gaactggata cctggaatca gaccgatttt
    20821 gtgaaggtga aaaaattcgc gggttactgg cagccaggat tgcccaaact ggacagcata
    20881 acctggcgtc cggtggcgga taacaacacc cgcgcggcaa tgctgcaaac cggtgaagcg
    20941 cagtttgctt tccccattcc ttacgagcag gccgcactgc tggagaaaaa caaaaatatc
    21001 gagttgatgg ccagtccgtc aattatgcag cgttatatca gtatgaacgt gacgcaaaag
    21061 ccattcgata acccgaaggt ccgtgaggcg ctgaattacg ccattaaccg tccggcgctg
    21121 gtgaaagtgg ccttcgcggg ctatgcaacg ccagctactg gtgtggtacc gccgagtatc
    21181 gcctacgcgc aaagttataa accgtggcct tacgatccag tgaaagcgcg cgaattactg
    21241 aaagaggcgg gatatcccaa cggtttcagt accacgctgt ggtcgtcaca taaccacagc
    21301 accgcgcaga aagtgctgca atttacccag cagcagttag cgcaggtcgg gattaaagcc
    21361 caggtgactg cgatggatgc cggacagcgg gcggcagaag ttgaaggtaa agggcaaaaa
    21421 gagagcggcg tgcggatgtt ctacactggc tggtcggctt caaccggcga agcggactgg
    21481 gcactatcgc cgttgtttgc ctcgcaaaac tggccaccga cgctgtttaa taccgcgttt
    21541 tacagcaata aacaggtgga tgacttcctg gctcaggcac tgaaaactaa tgatccggcg
    21601 gaaaagaccc gcttatataa ggcggcgcag gatatcatct ggcaagagtc gccgtggatc
    21661 ccgctggtgg tagaaaaact ggtgtcggca cacagtaaaa acctgaccgg tttttggatc
    21721 atgccagata ccggcttcag ctttgaagac gcggatttgc aataagcaac gcagggagtg
    21781 gaatgcttaa ttacgttatc aaacgattac tggggttgat tccgacgctg tttatcgtct
    21841 cggtgctggt gtttttattt gtgcatatgc tgcccggcga tccggcgcga ttgattgccg
    21901 ggcccgaagc tgatgcgcag gttatagaac tggtgcgtca gcagctgggg ttggatcagc
    21961 cgctgtatca ccagttctgg cactatatca gcaatgctgt gcagggggat tttggcctgt
    22021 cgatggtgtc acgtcgtccg gttgccgatg agattgccag gcgctttatg ccaacgctgt
    22081 ggctgaccat aaccagtatg gtctgggcgg tgatatttgg tatggcggcg ggaattatcg
    22141 ccgccgtctg gcgtaaccgt tggccggatc gattgagtat gaccattgcg gtgtcgggga
    22201 tctcgtttcc ggcatttgcg ctggggatgc ttttaattca ggtattctcc gttgaactgg
    22261 gctggctgcc taccgtggga gcagacagtt ggcagcacta cattttaccc tccctgacgc
    22321 tcggcgcggc agtggccgcc gtgatggcgc gctttacccg cgcgtcgttt gtcgacgttt
    22381 taagcgaaga ttatatgcgt accgcgaggg cgaaaggggt gagcgaaacc tgggttgtcc
    22441 tcaaacacgg gctacgcaac gcgatgatcc cggtagtgac catgatgggc ttacagtttg
    22501 gctttttgct cggtggttcc atcgtggtgg agaaagtttt caactggccc ggacttggac
    22561 gcttactcgt tgactccgta gaaatgcgcg attacccggt gattcaggcg gaaattctgc
    22621 ttttctcgct ggaatttatt cttatcaact tagtggtgga tgtgctttac gccgccatta
    22681 acccggctat caggtacaag taaggatgcg actatttaac tggcgacgtc aggcggtgtt
    22741 aaacgccatg ccactggtca aacctgacca ggtacgtaca ccgtggcatg aattctggcg
    22801 acgatttcgc cgtcagcata tggcgatgac cgccgcatta ttcgttattt tattgattgt
    22861 ggtggccatt tttgcacgct ggatcgctcc ctatgacgcc gaaaattatt ttgattatga
    22921 caatctgaat aacggacctt ctttgcagca ctggtttggc gtcgattcac tggggcgcga
    22981 cattttcagc cgtgtcctgg ttggtgcgca aatctcgctg gcggcgggcg tgtttgccgt
    23041 gtttatcggt gcggcgatag ggacgttgct gggcttgttg gctggatatt atgaaggctg
    23101 gtgggatcgg ctgatcatgc gcatttgcga tgtgctgttt gccttcccgg gtattttact
    23161 ggcgatcgct gttgttgcgg tgttgggaag cggcattgct aacgtgatta ttgcagtcgc
    23221 cattttttcc atccctgcgt ttgcccgcct ggtgcgcggc aacacgctgg tgttgaaaca
    23281 gcaaaccttt attgagtcag cacgcagtat tggtgccagc gatatgacca ttttgttgcg
    23341 tcatatcctg cctgggaccg tctcttctat cgtggtgttt ttcaccatgc gcattggtac
    23401 ctcgattatc tctgccgcca gcctgtcatt tctcggcctc ggtgcgcagc cgccgacacc
    23461 agagtgggga gcaatgctca atgaggctcg agcggatatg gttatcgcgc cgcatgtcgc
    23521 tgtttttccg gccctggcta tttttctgac tgtactggcg ttcaatttgt tgggcgatgg
    23581 tttacgcgat gcgctggatc cgaaaattaa aggatagtta cgtttgaata ttgcttgaaa
    23641 gagtaatcac ctcacaggaa attattgccc taagcaagtg ttgtaacttt ctgctgattt
    23701 tgtagaatcg ggtaatttgg ttaaatagcc gcagcaaggg actgtttttg cagcggcaca
    23761 gcgttcagac agttattttg ttaaatgtat taacatgctg agtttatacg aaaagataaa
    23821 gataaggctg ataattttat ttttattggc agcactgtca tttattggcc tttttttcat
    23881 cattaactat caactggtat cggaacgcgc ggtaaaacgt gccgatagcc gctttgaact
    23941 tattcagaaa aacgttggct atttctttaa agatattgaa cgttcggccc tgacattaaa
    24001 ggactcactg tatttattaa aaaatacaga ggagattcaa cgcgctgtga tcctgaaaat
    24061 ggaaatgatg ccatttttag actcggtggg actggtactt gatgataata aatattatct
    24121 cttttcgcgg agggcgaacg ataaaatcgt tgtttatcat caggaacaag taaatggacc
    24181 gcttgtcgac gagtcagggc aggttatttt tgccgatttt aacccatcga aacgaccgtg
    24241 gtcggtggct tcagatgact ctaacaacag ctggaatccg gcctacaatt gctttgatcg
    24301 tccgggtaaa aaatgtatct cttttacgct acgcatcaac ggcaaagatc acgatttgtt
    24361 agcggtggat aaaattcatg tcgatttaaa ctggcgatat ctgaacgagt atcttgatca
    24421 aatcagcgct aatgatgaag ttctattttt gaaacaaggc catgagatca ttgccaagaa
    24481 tcaactcgct cgtgaaaaac tgattattta taatagcgaa ggtgattata atattattga
    24541 ttctgtcgat actgaatata tcgaaaaaac atcagcggtg ccaaataacg cattattcga
    24601 aatctatttt tattatccgg gcggtaattt attgaacgca tcagataaac ttttttatct
    24661 gccgtttgcg ttcattatta tcgtattgct ggtggtttat ttaatgacca ctcgtgtgtt
    24721 ccgtcggcaa ttttctgaaa tgacagagct gattaatacg ctggcgtttt tgcctgactc
    24781 aacggatcag atcgaggctc tgaaaattcg cgaaggcgat gcgaaagaga ttatcagcat
    24841 caaaaattcg atcgcggaaa tgaaagatgc cgaaattgaa cggtcaaata aattgctctc
    24901 actgatctct tacgatcagg aaagtggttt tattaaaaat atggcgatta ttgagtccaa
    24961 caataatcag tatctggctg tggggatcat caaactgtgt ggtctggaag ccgtggaagc
    25021 tgtgtttggt gttgatgaac gcaataaaat cgtcaggaaa ttgtgtcagc gaattgctga
    25081 gaaatatgca caatgctgcg atatcgtgac attcaatgcc gatctctatt tacttctgtg
    25141 tcgggaaaat gtacagacat ttacccgtaa aatagcgatg gtaaacgatt ttgacagcag
    25201 ctttggctac cgcaatctgc gcatccataa gtctgccatt tgtgaacctt tgcaggggga
    25261 aaacgcctgg agttacgcag aaaaactgaa actggcgatt tccagtatcc gtgaccatat
    25321 gttctccgag tttattttct gtgatgacgc gaaactcaac gaaatagaag agaatatctg
    25381 gattgcgcgt aatattcgcc atgcaatgga aattggcgaa ctattcctcg tctatcaacc
    25441 gatcgttgat attaacaccc gcgccattct gggcgcggag gcgttgtgcc gttgggtgtc
    25501 tgcggagcgg gggatcattt caccgctgaa gttcattacc attgctgaag atatcgggtt
    25561 tatcaatgag ctgggttatc agattattaa aacggcaatg ggtgaattca gacattttag
    25621 tcagcgtgca tcgctgaagg atgatttctt actgcatatt aatgtttcgc cctggcagtt
    25681 aaacgaaccg cactttcatg agcgttttac caccatcatg aaagaaaatg gcctgaaggc
    25741 gaacagcctc tgtgttgaga tcactgaaac cgtgatcgag cgaattaatg aacattttta
    25801 tctcaatatt gaacaactgc gtaaacaagg gttacggata tcgattgatg actttggcac
    25861 cggtttgtca aacctgaaac gtttttatga aattaatcca gacagcataa aggtggactc
    25921 gcaatttacc ggcgatattt tcggtactgc gggaaaaatt gtgcgcatta ttttcgacct
    25981 ggcacgctat aaccggatcc cggtgattgc ggaaggcgta gagagcgaag acgttgcgcg
    26041 cgaattaatc aaattaggat gtgttcaggc tcaggggtat ctgtatcaga aacccatgcc
    26101 attctccgcc tgggataaaa gtggaaaatt agtaaaagag tagtttacgt atgtccagaa
    26161 tcaataagtt cgtacttaca gtcagtctgc tgatttttat catgatttca gcagttgcct
    26221 gcgggatcta cactcaaatg gtaaaggaac gggtgtatag cctgaaacag tccgttattg
    26281 atactgcttt tgcggtggca aatattgctg aatatcggcg tagcgtggca attgatctta
    26341 tcaacacgct aaatcccacg gaggaacagc tgttggttgg cttacgcaca gcttacgccg
    26401 actcggtttc accctcttat ttgtacgatg tcggtcctta tctgatttcc agtgacgaat
    26461 gtattcaggt aaaggagttc gagaaaaatt attgtgcaga tattatgcag gttgtgaagt
    26521 atcgacatgt caaaaataca gggtttatct cttttgacgg taaaaccttc gtctattacc
    26581 tctatccggt aactcacaat cgtagtctga tatttttgct tggtctggag cgtttttctt
    26641 tactgtcaaa atcgctggcg atggacagcg agaacctgat gttctctctg tttaagaacg
    26701 gtaaaccggt gaccggtgat gaatataatg ctaaaaacgc catcttcacc gtttcggaag
    26761 cgatggagca cttcgcctat ttgccgaccg gattgtacgt atttgcgtat aaaaaagatg
    26821 tttatttgcg ggtctgtaca ttgattattt tctttgccgc attggtggca gtgatatcgg
    26881 gtgccagttg cctctatctg gtacgcagag tgattaatcg tggtattgtg gagaaagaag
    26941 ccattattaa taaccatttt gaacgcgtac tggatggcgg gcttttcttt tcggctgccg
    27001 atgtcaaaaa actctacagt atgtataact cggcgttcct ggacgacctg accaaagcaa
    27061 tgggtagaaa atcctttgac gaagatttaa aagcgctgcc ggaaaaaggc ggttatttgt
    27121 gcctgtttga cgtcgataaa ttcaaaaata ttaacgacac cttcggtcat ttgctgggcg
    27181 atgaagtgtt gatgaaagtg gtgaaaatcc ttaaatcaca gatcccggta gataaaggta
    27241 aagtctaccg cttcggcggt gacgaatttg cggtgattta tactggcggc acgctggaag
    27301 agttactatc gattctaaaa gaaatcgttc atttccaggt gggaagcatt aatttaagca
    27361 ccagtatcgg tgtagcgcat tcaaatgaat gtactaccgt cgaacgcttg aaaatgctgg
    27421 cggatgagcg gctgtataag agtaagaaaa acggcagggc tcagattagc tggcagtaga
    27481 attagaatag tgttagtacc cctgaatcaa ggggtactaa tgaatgtggt aaatcatttg
    27541 caaacatttt actttgcaaa aacttccggc ccttttattg ccaatgtatt caaaatacgt
    27601 agtgccgggc cgctcggttt tatttcatta cgttcccatt tcgagacact ttcttttgac
    27661 attcccattg tatgtgccag cacggattgt gacatgccgt atcgggtgcg gatggctttg
    27721 atctcttcac ccgtcattga tttgactttg gctatacggg cgctgacttc acgggctttg
    27781 accatgacgg caatggcatc aacctcacta tcagacatca ggcccagtgc attcagttcc
    27841 tttgcatctt ccagcatcat ttgtaattta tcgctcattt ttcacctcaa tcaagaagcc
    27901 agtttcgatc aaatcctcta tttgcttatt gctaaaggcc agaaatgcat ttgccatttt
    27961 tttgtaggct gccagttcat catcttcgat ttctttgctt cctttactgc ttagaccact
    28021 cttggaccaa ccatcataaa aaaacacatg atttgctgat ttaaaaaaga taattgttct
    28081 ggctccacca ctctttcctt gttggagggg taaccgtttt ttgatgacac cgctacctaa
    28141 atcagcctcc catatgcctt gcatcacttc ttcagcggca gccatgagaa ctttatcgtg
    28201 aataccaagt tttttcgtgc tttggctgaa tcgccttgcc ttatacacac ccatacatcc
    28261 atccttgtgg taatgtttac tatagtaacc attacgctta tttcaagtaa ttgcgatcaa
    28321 aactttaatt tggtgtgaaa aagtgagagt tgcctcgagt acgattcatt tttctcgatt
    28381 tatgcgtcag gggattttgt gcgtaagggt aggacatcgg gaagggtagt agaggcagat
    28441 cgaagaacaa tatccttgtt agatctgcct ttagattaat taaacccggc taccccacaa
    28501 atcgtactca tcggcgtgct cgactttcac acgcaggata tcacccggct taacgttggt
    28561 ttcaccattg agataaaccg caccgtcgat ttccggtgca tctgccatgc tgcgaccaat
    28621 cgcgccttct tcgtccactt cgtcgataat caccagaatt tcacggccca ctttctcttg
    28681 caggcgctcg gcggaaatct gctgctgcaa ctgcatgaaa cggttccagc gttcttcttt
    28741 cacttcttcc ggaacctggt caggcagggc attggcgtct gcaccttcaa ccgggctgta
    28801 tttaaagcag ccaacgcgat ccagacgcgc ttctttcagg aagtcgagta gcatctggaa
    28861 atcttcttcc gtctcgccag ggaagccgac aataaaggtt gagcgtaggg tcagttccgg
    28921 gcagatttcg cgccactgtt tgatgcgcgc cagttggcga tctacagaac ccggacgctt
    28981 catcagtttg agaatgcgcg ggctggcgtg ctgcaacgga atgtccagat acggcaggat
    29041 tttgccttct gccatcagtg ggatgacgtc gtccacatgc ggataagggt aaacgtagtg
    29101 cagacgtgtc cagatcccca gtttcgataa ctgttcgcac aggctgacca tgctggtttt
    29161 taccggctcg ccgttgtgga agccagtacg atgtttaaca tcaacgccat aggcggaagt
    29221 atcctgcgag atcaccagaa tctctttaac gcccgcatct accagacgtt tcgcttcact
    29281 taatacttcg ccaatcggac ggctcaccag gtcgccgcgc atagacggaa taatgcagaa
    29341 ggtgcagcgg tgattacagc cttcagaaat tttcagatag gcataatgac gcggcgtcag
    29401 tttgacacct tgttctggca ccaggctcag gaatgggttg tgtttcggtt ttggcacgta
    29461 gtgatgaacg tgctccagaa cctgctcata gctatgaggc ccggtgattt ccagcacttt
    29521 cgggtggact tcgcggatct gatcttcttt tgcccccaga caaccggtca caattacctt
    29581 gccgttttca ttcaacgctt caccaatggc ttccagtgat tcttgtaccg cgctgtcaat
    29641 aaagccgcag gtgttgacga tcaccatgtc cgcatcgtca tagctcggta ccacgtcata
    29701 accttcagtg cggagttcgg tgagaatacg ctctgaatcg acaaggtttt tcggacagcc
    29761 aagggaaaca aagccgattt tcggctgggg agttactttg ctcatagctt aaaaaatatt
    29821 cagttacagg aaaggtcagg gcagggattc tacagagttc tggataaaat ttgtatcgca
    29881 atctcattcg ctggcggagg cgaaggaaat gtaaattttg ttaattcggc gtgaagaatt
    29941 gatcctggac agcattatgc tcaaaaaata gccatactat ttaattgcaa caaggctgga
    30001 aagaggagga tcgaagtatg ttcgttgaca gacagcgaat cgatctgctg aaccggttga
    30061 tcgacgcacg cgttgacctc gccgcatacg tgcaactgag gaaggcaaaa ggatacatgt
    30121 ccgtcagcga aagcaatcat ctacgagata acttttttaa actgaatcgc gaactgcacg
    30181 ataaatcgct gcggttgaat cttcatctgg atcaggaaga gtggagtgct cttcatcatg
    30241 ctgaagaagc attagcgaca gccgcagtat gtttgatgag tgggcaccat gattgcccga
    30301 ctgttattac cgtcaacgcc gataagcttg aaaattgtct gatgagctta acgctgagta
    30361 tccagagcct gcagaagcac gccatgcttg agaaggcctg aaaactaagg gggagaaagc
    30421 gtctccccct tcatgtttaa gtttttgtaa aaatgaattt gttatctcct ccactgacta
    30481 cgctttaagc cagagtcaat ccggaggcgt tatgcatcga caattctttt tccttgtgcc
    30541 ccttatttgt ctttcttccg ctctctgggc ggctcctgca acggtaaatg tcgaagtact
    30601 gcaagacaaa ctcgaccatc cctgggcact ggccttttta cccgataacc acggtatgtt
    30661 aatcactctg cgcggcggcg agttgcgtca ctggcaagca ggaaaaggat tatctgcgcc
    30721 gctttccgga gttccggacg tttgggcgca cgggcagggc ggcctgctgg acgtggtttt
    30781 agcgcctgat tttgctcagt ctcgccgcat ctggttaagt tattccgaag ttggcgatga
    30841 tggcaaagcc ggaactgctg tggggtatgg ccgcttaagt gatgatctct caaaagtgac
    30901 cgactttcgc accgtctttc gccagatgcc aaaactgtct accggcaacc attttggcgg
    30961 gcggctggta ttcgacggta aaggttatct ttttattgct ctgggcgaaa acaatcagcg
    31021 cccgacggcg caggatctgg ataaattaca gggcaaactg gtgcgtctga ccgaccaggg
    31081 cgaaatcccg gatgataatc cttttataaa ggaatccggt gcgcgcgccg agatctggtc
    31141 ttatggcatt cgtaatccgc aaggaatggc gatgaatccg tggagtaatg cactgtggct
    31201 gaatgaacat ggcccgcgcg gtggtgatga aattaatatc ccgcaaaaag gcaaaaacta
    31261 cggctggccg ctggcaacct ggggaatcaa ctattcaggc tttaagatac cggaagcgaa
    31321 aggggagatc gtcgccggga ccgagcaacc tgttttttac tggaaagatt cgcccgccgt
    31381 gagcggcatg gccttctata acagcgataa attcccccag tggcagcaaa aattatttat
    31441 tggcgcgctg aaagataaag atgtcattgt gatgagcgtc aacggcgaca aagtaacaga
    31501 agatggccgt attttaacgg acagagggca gcgaattcgt gatgttcgca ctggacccga
    31561 cggttattta tacgttctca ccgacgagtc cagtggggaa ttacttaaag ttagcccacg
    31621 caattagctg acgggaatca tcaccacttt gcgcactgcg gggcgttcag tgagttgctg
    31681 ataccagcgt tgcagatttg gacgcggtgt ccaggtcagg ccaacattaa acaaattgta
    31741 gatgaacggt gcgatagcga tatcgcccac accaaactcg tcgccagaga accattttac
    31801 ttttgccagt tccgcatcga gcagggcaaa cagggcgtcg cactctttgc aactggcatc
    31861 aatggcggcc tgatcgcgct cttccggtgg tgttctgact aatcccatca ggatcccgcg
    31921 atgagcatta ctgagtgtct ggtttgccca gtccatccat ttttctgctt ccgcacgacg
    31981 tgccggtgag tcgatccaca ggcgtttttg cccgtactgt gccgccagat agcggacaat
    32041 ggcgtttgat tcccaaagaa tgagatcact ttcgtcgtca cgcaacaacg gcaccagccc
    32101 gttagggttc atcgccagaa aatcagcatc gtgatttatc ccaaactcac ggcccgcgag
    32161 aatttgctca taaggtagtt ccagttcttc gagcgtcagc aatacttttt ttacgttggt
    32221 tgaattattc cgaccccaca gcgtaatcat actcacccct tttccgacag gcagccacag
    32281 gctgaacagg actccgatgg tgaagtaaag ttaactttta agcaacagct ggcaaaaaaa
    32341 tgacaccaga agcagcgaag cggcaggata ttgcataaac tttaaaaact ttaccaactt
    32401 acggtttctt taagtttgtg tgtgcgttat taatcaccga acttatcata cggcgatata
    32461 acgtattttt ttgaatggat actcgggtgg catttatgac gcaatactcc tctctccttc
    32521 gtggtcttgc agcgggttct gcatttttat tcctttttgc cccaacggca ttcgcggccg
    32581 aacaaaccgt tgaagcgccg agcgtggatg cgcgtgcatg gattttaatg gattacgcca
    32641 gcggtaaagt gctggcagaa ggcaatgcgg atgagaaact ggatcccgcg agcctgacta
    32701 aaatcatgac cagctatgtg gttgggcagg cgcttaaggc cgataagatt aaactcaccg
    32761 atatggtgac ggtcggtaaa gatgcctggg cgacgggaaa tccggcactg cgtggttcat
    32821 cggtaatgtt cctcaaaccg ggcgatcagg tttcggtggc agacttgaac aaaggtgtga
    32881 ttatccagtc cggtaatgac gcctgtattg cgctggccga ttacgttgcc gggagccagg
    32941 agtcatttat tggtttgatg aatggttatg ccaaaaaact ggggctgacc aacactacct
    33001 tccagacggt gcacggtctg gatgcgccgg ggcagttcag taccgcgcgc gatatggcat
    33061 tgctgggtaa agcattgatc cacgatgtgc cggaagagta cgccattcat aaagagaaag
    33121 agttcacctt caacaaaatt cgtcagccta accgtaaccg tctgctgtgg agcagcaatc
    33181 tgaatgttga tggcatgaag acaggaacca cggcaggcgc gggatataat ctggttgctt
    33241 cggctaccca gggcgatatg cgtttaatct ccgtagtgct gggggcgaaa accgaccgta
    33301 tccgttttaa tgagtctgag aaattattga cctggggttt ccgcttcttt gaaaccgtga
    33361 cgccaattaa acctgatgcc acctttgtga ctcagcgcgt ctggtttggt gataagagcg
    33421 aagtgaatct cggggcaggc gaagcgggct cagtgaccat accgcgtggg cagctgaaaa
    33481 acctgaaggc gagctacacg ttaacggaac cgcagcttac cgcaccgctg aaaaaaggtc
    33541 aggttgtcgg gaccattgat ttccagctta acggtaaatc cattgagcag cgtccgctga
    33601 tcgtgatgga aaatgtggaa gagggcggat tctttggtcg gatgtgggat ttcgtgatga
    33661 tgaaattcca tcagtggttc ggcagctggt tctcttaatc ttctgataac cggatggcgc
    33721 gaaacgtcat ccggttatac gtcattaata catcaactta atacgctgcg tctgcgcgta
    33781 cttcacatac tcatcttccg ggcaacaatc gctcaccaca atatcaaagc gtttcaggtc
    33841 acccatgcgc gccggacgca ccttgccaaa tttactgtgg tcaacaacca gcacatgctt
    33901 ttgcgccatc gacatggccc agtgttttac cggcaactct tcaagattaa aacaggtagc
    33961 gcctttactg acatgcacgc ccgccgcaga ataaaaagcg atatccgggc aaaaattatt
    34021 cagcgtttgc tgaaaatcga tgggtttgaa aatggcgttg ctggcgtgaa attcaccacc
    34081 gcacagaaat gcgcggcaat ggggtttttc tttcagcgcc agaaaggtat ttagcgaata
    34141 acaaacggcg gtaaaaggga tttcattatc aatcgcttca ataatccacg gcgtggtggt
    34201 gccacagtca aaaaagaggg tctgatcggg ttctaccagt gtcgcagcca gttttgccgc
    34261 ccggcgtttt tcttccacca ggcgggattt ttgatcgctt aacaggtaat ggctggcgct
    34321 gcgcggttcc agaacaatat agccgccgag caaaacgacg ggcgcactgt ggttgttcag
    34381 atcgcgacga atcgtcatct ccgaaacccc aagcagggcg gcggcgtctt taagatgtaa
    34441 cttatcgctg cgttttaatt cttgcagcag ctgcccgata cgctcttcgc gacgtgtttc
    34501 cataatccct ctgaatagtt attgaagcga gccgctcaat actacacttt ttagcagaga
    34561 tcagtcacgc acccagcctt tgcggatcgg taatgcaaaa cagaagcgat accacgattg
    34621 cagacgttga tagagtttat gccccatcgc ttgccagata atctgggcac taaggcagcc
    34681 aatcatacct gccagcaatc caccgagcat atccagcggc cagtggacgc caagataaac
    34741 gcgcgaccag gcaatgacga cggccagcac cattaaaagt gagccggacc acaggcgatg
    34801 ccagcataaa aatgccagtg caaaggtgaa aatcaccgta ccgtgatcgc tcgggaatga
    34861 gtcatccgcc gcatgatgca ggaagttata gccgatattt tcgacaaacg gtcggtcgtg
    34921 cggaaaaaga tgtcccatcg tccaggacac caacaggctg accgccagcg cgatagcgat
    34981 ttttatcacc agttgccgtt gtgctgtaag cccccacaac caaagtaccg cggccagcag
    35041 cggcaccacg gtaatcaaat ctttagcaat aaaaatcgcc aacgagatca tccacggagc
    35101 cgagtctggc gtcgcgttaa taagagagaa tagagagaga ttcaaatttt ccagcataac
    35161 ttcccgacgc aaagtgatta aaagggagcc aatacaggca agtcgttgag aataaagtgc
    35221 aggttaaact gggtaaagcg gcatcgtctt atttccctca agcggcctgt ttacggtggg
    35281 tgattgtaac gggcataggt taaataaaac ttaaagaaag cgtagctata ctcgcaataa
    35341 tgtaagaatg tgcttaaccg tggtttcagc tacaaaattc gctttctcgt tagctgcgct
    35401 tttattaaac tctgcgcgat tattattggc gaagaaattg catgcaaaat aaattagctt
    35461 ccggtgccag gcttggacgt caggcgttac ttttccctct ctgtctggtg ctttacgaat
    35521 tttcaaccta tatcggcaac gatatgattc aacccggtat gttggccgtg gtggaacaat
    35581 atcaggcggg cattgattgg gttcctactt cgatgaccgc gtatctggcg ggcgggatgt
    35641 ttttacaatg gctcctgggg ccgttgtcgg atcgtattgg tcgccgtccg gtgatgctgg
    35701 cgggtgtggt gtggtgtggt ttattgtcac ctgtctggca atattactgg cgcaaaacat
    35761 tgaacaattc accctgttgc gcttcttgca gggcataagc ctctgtttca ttggcgctgt
    35821 gggatacgcc gcaattcagg aatccttcga agaggcggtt tgtatcaaga tcaccgcgct
    35881 gatggcgaac gtggcgctga ttgctccgct acttggtcca ctggtgggcg cggcgtggat
    35941 ccatgtgctg ccctgggaag ggatgtttgt cttgtttgcc gcattggcag cgatctcctt
    36001 tttcggtctg caacgagcca tgcctgaaac cgccacgcgt ataggcgaga aactgtcgct
    36061 gaaagaactc ggtcgtgact ataagctggt gctgaagaac ggtcgctttg tggcgggggc
    36121 gctggcgctg ggattcgtta gcctgccgtt gctggcgtgg atcgctcagt cgccgattat
    36181 catcattacc ggcgagcagt tgagcagcta tgaatatggt ttgctgcaag tgcctatttt
    36241 cggggcgtta attgcgggta acttgctgtt agcgcgtctg acctcgcgcc gcaccgtacg
    36301 ttcgctgatt attatgggcg gctggccgat tatgattggt ctgttggtcg ctgctgcggc
    36361 aacggttatc tcatcgcacg cgtatttatg gatgaccgcc gggttaagta tttatgcttt
    36421 cggtattggt ctggcgaatg cgggactggt gcgattaacc ctgtttgcca gcgatatgag
    36481 taaaggtacg gtttctgcgg cgatgggaat gctgcaaatg ctgatcttta ccgtcggtat
    36541 tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga ctgtttaatc tcttcaacct
    36601 tgtcaacgga attttgtggc tgtcgctgat ggttatcttt ttaaaagata aacagatggg
    36661 aaattctcac gaagggtaaa aaaatgtctg attgctttgt gcgatcaggc attctcgaat
    36721 taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc cttcacctac gctggcaaga
    36781 tggcgaacat aaggatgcgg gtggtaagcc ggagctggcg caggagccac atagacggtt
    36841 tgcggcacag tcgcgacgct gaccgcttgt ggaacgctga ctgaatcagg aaccacaacc
    36901 actttgtatc cactcggcac atcgacggtg acactttgcg ccatcgccac accagtaaag
    36961 cccattaaaa gcgcgcccaa cagtagacaa tttttcataa tcatttcgcc tgaaataatg
    37021 cagcgggtta atttccgcga attatgcagc cttcattcag gtattgtgtt gccgggcgtg
    37081 ctgccgtttt tattggtcaa atggcgcttc gtgtttaaga actttatcga tcacatccag
    37141 tacgccttca cggttattgg agcctgcccg gtattttgcc gctgcgacga ccgcgttgcc
    37201 ggcattttcc attgcaaaac taaagcctgc ctgacgcagc atctcaatat cgttaccgcc
    37261 atcgccaaag accaccactt cgctgtcgtc tattccccat aatttctgca gttggcgaag
    37321 gccattggct ttatgtacgc cggggataat cagatcgatg ctgccgttgc cggtgtggac
    37381 cggcaccata atatcgccga tggcctcatg taatgttttt tgtacttgtg gaatcagttc
    37441 atcggaaaga ttcaggccaa acttaaagaa gatatcttct aagttgtcaa agttatcgac
    37501 gtattccaga cggtgataat acatttccgc caccattttc atggcatcgt catatttttt
    37561 gagcgtatag gcgctatttt ttccgcaggc aataatttcc acttccgggc gcgtcagcaa
    37621 atgttccacg acagtagtaa acgcatcctt cgacagctcg ccattaaaaa catctttgcc
    37681 ttcactcact acccagccgc cgttttcagc cacaaaggca atttcattag caatttcagg
    37741 gaagaaagag atcaactgat aatattgatt cccgctggcg accacaaagc gaatgccttg
    37801 tgctttcatt tgctgatact gagccataaa ccgctcacgg ttataggttt tttgatcgct
    37861 taagaaagta ccatccatgt ctaccgcaat taatttaatg ctcatcaact attctccatc
    37921 gccgtctgcg ttttggtatc gggtttggcg acggctttcg cgacaatagc cgcgagaata
    37981 accagcgcca gtacaaccag cattgcacta cgtaatccat aatgttcgcc gagatagccc
    38041 agcagcggcg gcccgacgag gaaagccaga taaccggtcg ttgctaccac actgacgcgg
    38101 gttggtgcat cggggccggt atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag
    38161 gcacccagtc cccagagtac aacagacacc ccagcgaccc aggcgctatc gacaaaaata
    38221 atcagcccaa tacccaacgc ccccattagc gcactggccc gaaccacggc aacgcgactg
    38281 taacggtcga tgaaccaacc gccagtgaag cgtccaacgg tcatccccag ggtaaaaccg
    38341 gcataaatca gcgagccgga agtggggcta aaaccgtgac cgtcaaccat taataagggt
    38401 aaccagtcgt tggcagaacc ttcggcaaag gccatcgcca gcaccacaac accaatcagc
    38461 agcaactgga tatcgcgata aaaaggtacg cctttttcgc catgctgggt gccatcggca
    38521 gcatttttgc ccgtaccgtc agggattgcc tgaatggcga tatatatagg cgcgatgcct
    38581 accagcgccg ccaataaaat gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc
    38641 ccgacaccag cgcctgccag cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc
    38701 gttttattca tttctcgctc aacggcggca ccttcaacgt ttatcgccac ttccgcagaa
    38761 ccaaaacttg ccccaaagac gccgagacca acggcaaaga gcaggggcga tgtcagccag
    38821 agtgccagac ttaatatcat catcccgacc aatgcgcagg acatggtgac caggatgaca
    38881 ttacgtgtcc caaagcgttt cactaaccac gccgagcaga gaataccgct catcgaaccg
    38941 atcgacagac caaagagaac accgcccatt tcagcgattg agacagagag aatatcgcgg
    39001 atagcaggcg tacgggttgc ccaggacgcc attaacaggc ccggcaaaaa gaagaacata
    39061 aacagcgccc aggttcggcg tttcaatgca ttacgtgaag aatttacggt catagatcac
    39121 gtcaaaataa gaagagggaa gacaacatta gcaaggttgt gtacatttgt acacaattgc
    39181 agacagagga aatgacatgc gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca
    39241 ggccacgctg gaggcggtga aactttacgg aatacatgct gttacgcacc gcaaaattgc
    39301 tacccttgcc ggggtaccgt tgggatcgat gacctactat ttttcaggaa ttgatgagtt
    39361 gttgctggag gcgttcagca gttttactga gatcatgtcc cggcaatatc aggcattttt
    39421 tagcgatgtt agtgatgctc aaggcgcatg ccaggctatc accgatatga tctacagctc
    39481 acaggttgca acgccggata acatggagct gatgtaccag ctctacgcgc tggctagccg
    39541 aaaaccgcta ttaaaaacgg taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga
    39601 acaatggttt gaacccggaa ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct
    39661 gcattttgtc accgaccgta agccgctatc gcgcgaggag attttgagga tggttgagag
    39721 ggttgcaggg tagtagataa atttcaggca acaaaaaacc catcaacctt gaaccgaaat
    39781 ggcggggttg atgggctcca caaaatgggg acatcaaaga aaagcagtgg cactaattaa
    39841 gactgatgcc ctgcggaaaa gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac
    39901 ttcagtttta tcctaatcct ggccatacca tgacgatgat tgtccctgcc agcgtcagca
    39961 ggacgttggc gattgcgtag gtgcccgcat agcccagcgc cgggatgtta ctgcgagctg
    40021 tatcactgat gatctccatt gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca
    40081 acagtgcgcg gttcattcgc aatacataag caccgaacaa gaaacagata accacgggca
    40141 caagactgac aatcaatccg gcaatcaaca tctgaccgcc aatcgcgccc agaccgttat
    40201 taataccgct accggcgctc agaccaacgc ctgccataaa caccatcaag ccgaactctt
    40261 tcaccatgct taatgcccct tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa
    40321 agcccagcat aattccggcg aataacaacc cggcagcgtt ccccatgccg aaactgaatg
    40381 tgctgaactg gaaggtgatc atcccgatca tcagcccaat aacaaagaag gcgcagaatg
    40441 ccagcaggtc agtgacctgg ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt
    40501 ttacgcggcg ggcatcgccg ctgacttgta aaacgtcacc tttgttaagc acgacgttgt
    40561 catcaatcgg catctcaatc tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg
    40621 tcaacttcag ttgtgcgaga cgtttaccta cagcgttatg gtttttaacg accacttctt
    40681 cagtgacgat acgcatgtcg agaaggtcac gatcgaaaac ttctttaccg ttacggaagc
    40741 tgggatcgag tcgggcatgg gcgtcgggat agcctaccaa cgctatttca tcgcccattt
    40801 gtagcacggc atcaccgtct ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt
    40861 agcagccggt ttgtcgataa atacccagtt cacgcagatt tttgccgtcg gtccaggcca
    40921 ccagctccgg gccgacgcga taggcgcgga tcaccggtaa ataaacctta cggttggcat
    40981 cagtgtccag gccacgttcg cgggcgattt gctgggcgct ggtctgtaag tcctgatgct
    41041 gcaatttcgg caagtaacgc gcaccaacaa tcaaactcac cagaccgatt aaataggtta
    41101 aggcataccc gaggctcaga ttatccagtg ccagtgagag ctgcctgctt tccataccgg
    41161 aatgacgcag tgtatcgcca gcaccgacca gaaccggtgt cgacgtcata gagcctgcta
    41221 acataccggc cgtcaggcca atatcccagc caaacagctt acctaaccct aaggcgatca
    41281 ccagcgcact gccaaccatc accagtgcta acattaggta atttttccca tcgcgaaaaa
    41341 aaatggaaaa aaagttcggt ccggcttcga ccccgacgca gaaaataaac agcataaagc
    41401 caagattaag cgcatcggtg ttaatgctga aatgttgttg gcctaataac agcgatacga
    41461 ctaaaacgcc aatggaatta cccagttgga tcgaaccaag tcgtaacttt ccgagacata
    41521 gcccaagcgc gaggaccaca aataataaca gaatgtaatt cccatttaac aattcggcga
    41581 cgtttatatt cacggaggtt aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa
    41641 tttacgataa tgttttttac cagaattcag ggcgcagatt cattcagcgc acctaaacga
    41701 tagtaaagta acaatatatt ttactagtgt aatcacatta ggtatcaacg gctatatgaa
    41761 ttgcgttggc ctatattagc atggaatgcg aagcggcttt atcttactga acgccacact
    41821 ggcgaaaaat gtgttcgata gacgcagtgt caggaggaac gagtgaaaca taaacaacgt
    41881 tgggcggggg caatctgctg ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg
    41941 cacatgaaag gcgcttttcg ggctgccggg catcctgaaa tcggcttgct gtttttcatt
    42001 cttcctggag cagttgccag tttcttttca cagcgtagag aagtcctgaa acctctattt
    42061 ggcgcaatgc tggcggcacc ctgttcaatg cttattatgc ggctgttttt ttcaccgacc
    42121 cgctcattct ggcaagagct ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg
    42181 gcactgtgtt tcttatttat cagtagcttg tttaaaccac agcacagaaa aaatcagtaa
    42241 agccctcaac gcgagggctt gtcagacgat caggcgtcca gattctcttt cacccatgca
    42301 gcaaaatcgg tatagccgcc gatatgttgc tgatcgacaa aaatctgcgg cacggtttct
    42361 acgggtttac ctgccttttg ttgtagatct tctttagtga tcccttccgc acgaatatct
    42421 acatactgat actgaaaatc atcgcgttca ttgctcaatt tctcagccag atcttttgca
    42481 cgcacacagt aagggcaacc cggacgacca aaaataacgg tttgcattat ttctctcctc
    42541 atagatttat gcctgtaatg atcacgctaa aatgtattcg ctgaaagtag gtttaacctg
    42601 ttgcattaat tgctaaaagc tataactgtt aaacacaata cagtgaaaag ttttagactg
    42661 aaggttcact ttgcagaggg aagcgtatgc gcgcgatcgg taaattgcct aaaagcgtgt
    42721 tgatactgga atttatcgga atgatgttgc tggcggtggc gctgctgtcg gtaagcgact
    42781 ccctgtcgct gcctgagcca ttttctcggc cagaagtgca gattctgatg atttttctcg
    42841 gtgttttgct catgcttccc gctgcggtgg tggttattct tcaggtggca aaacgtcttg
    42901 ccccacagct gatgaaccgt ccaccgcagt attcacgttc agaaagagaa aaagataatg
    42961 acgccaacca ttgaacttac ttgtggccat cgctccattc gccatttcac tgatgaaccc
    43021 atttccgagg cgcagcgtga ggcgattatt aacagcgccc gtgcgacgtc cagttccagt
    43081 tttttgcagt gcagtagcat tattcgcatt accgacaaag cgttacgtga agaactggtg
    43141 acgctgaccg gcgggcaaaa acacgtagcg caagcggcgg agttctgggt gttctgtgcc
    43201 gactttaacc gccatttaca gatctgtccg gatgctcagc ttggcctggc ggaacaattg
    43261 ctgctcggtg tcgttgatac ggcaatgatg gcgcagaatg cattaaccgc agcggaatcg
    43321 ctgggattgg gcggggtata tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa
    43381 ctgctgaaat taccgcagca tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg
    43441 gataatccgg atcttaagcc gcgtttaccg tcctccattt tggtgcatga aaacagctat
    43501 caaccgctgg ataaagacgc actggcgcag tatgacgaac aactggctga atattacctc
    43561 acccgtggca gcaataatcg ccgggatacc tggagcgatc atatccgccg aacaatcatt
    43621 aaagaaagcc gcccatttat tctggattat ttgcacaaac agggttgggc gacgcgctaa
    43681 aaccgccacg ccgatgtatg atacgcgggc ttttgaccag gtctgacaga gaggtgcagg
    43741 gtgaaaattg ccatattgtc ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa
    43801 gccgctatac agcgcggtca cctggttgaa attcttgatc cgctttcttg ctacatgaac
    43861 ataaatcctg cggcgtcttc tattcactac aaaggccgca agttacccca ttttgacgca
    43921 gtgatcccgc gtattggcac cgccattacc ttttatggga cggcggcact gcgccagttc
    43981 gagatgctgg ggagctatcc gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa
    44041 ttgcgttcca tgcaactgct ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg
    44101 cattcgccgg atgataccag cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc
    44161 aagttggttg aaggcacgca gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg
    44221 gaaagcgtga ttgacgcttt ccgcggtctg aacgcgcata ttctggtgca ggaatatatc
    44281 aaagaggcgc aagggtgcga tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg
    44341 attgaacggc gggcgaaaga gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca
    44401 agcgtcgcca gtatcacacc acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg
    44461 gcgctggacg ttgctggtgt ggatattctg cgtgctaatc gcgggccgtt ggtgatggaa
    44521 gtgaatgcgt cgccggggct ggaaggaata gaaaaaacca ccggtatcga catcgcgggt
    44581 aaaatgatcc gctggatcga acgccacgct acgacagaat attgcctgaa aacgggtggt
    44641 tagtcgcaat cacattactg atcatggttt tgcctgctct ttttgcgtaa gctgtgccgg
    44701 tctttttatc gaaagaggtt gtacaaaatt atgacatcgc tggtcgttcc tggtctggat
    44761 acgctgcgtc aatggctcga tgacctgggg atgagttttt ttgaatgtga taactgtcag
    44821 gctctgcatc tgccccatat gcagaatttc gacggtgtct ttgatgccaa aatcgatctg
    44881 atcgataaca cgatcctgtt ttctgccatg gcggaagtcc gaccttcagc cgtattgccg
    44941 ctggcggcgg atttatctgc catcaatgcc agttcgctga ccgtgaaagc atttcttgat
    45001 atgcaggatg ataatctgcc aaagctggtg gtttgccagt ctttatccgt tatgcagggc
    45061 gtaacctatg agcagtttgc atggttcgtg cgtcagagcg aagagcagat ttcgatggtc
    45121 attcttgaag ctaatgccca tcaactgctg ttaccgactg acgatgaagg gcaaaacaac
    45181 gttaccgaaa actatttcct ccactgataa ctcctttcga gcacgcagtc gctggtgcag
    45241 tggctgcgcg ctgcaaaatt atctgctgtt tttaaccttt tcttaaagat tatttcactt
    45301 ctcttgtgtc gatttggctt tatcacatag agcaaatacg cataaaaatt tgttaaatac
    45361 cgttttttaa tccgagctat agtctcaaac cctggctaaa gttattcttg cgatgctttt
    45421 atatagcgag cagtgctggc cgggagagag ttctcttttc ttacaccgcg ccgataaaaa
    45481 atatgcacgt ttattgcata tctttcagtg tgacaacttt tgttcgtttg ttaacgaact
    45541 ttcagaagga aagagatatg accgccttaa ataaaaaatg gctttcgggt ctggttgcgg
    45601 gtgctctgat ggccgtctct gtcggcacgc tcgcggctga acaaaaaaca ctccacattt
    45661 ataactggtc tgattatatc gcccccgaca cggtggctaa ctttgaaaaa gaaaccggta
    45721 ttaaagtcgt ctacgatgtt ttcgactcta acgaagtact ggaaggcaaa ttaatggccg
    45781 ggagcaccgg ctttgacctg gtggtgccgt cagcaagctt tcttgaacgc cagctgacag
    45841 cgggagtttt ccagccgctg gataaaagca aattgccgga gtggaagaat ctcgacccgg
    45901 aactgctgaa gttggtcgcc aaacacgatc ccgacaataa atttgctatg ccctatatgt
    45961 gggcgacgac cgggattggc tataacgtcg ataaagtcaa agcggtgctg ggcgaaaacg
    46021 cgcccgtcga tagctgggac ttgatcctca agcctgaaaa tctggaaaaa ctgaaaagct
    46081 gcggtgtatc tttcctcgat gcgccagaag aagtttttgc taccgtgctg aattatctcg
    46141 gcaaagatcc caacagcact aaagcggatg attacaccgg accggctacc gatctgctgt
    46201 taaagctgcg cccgaacatt cgttatttcc attcatctca atatattaac gacctggcaa
    46261 acggcgatat ttgcgtcgct atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg
    46321 cgaaggaagc gaagaatggc gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg
    46381 cgttctttga tgtattcgcc atgcctgcgg atgccaaaaa taaagacgaa gcctatcagt
    46441 tcctgaatta cctgctgcgt cctgatgtgg tggcacatat ctctgaccat gtgttctatg
    46501 ctaacgccaa taaagcagcc acgccgctgg tgagtgcgga agtccgtgat aacccgggta
    46561 tttatccgcc tgcggatgtc cgcgcgaagt tgttcactct gaaagtacag gctccgaaaa
    46621 tcgaccgtgt gcgcacccgc gcgtggacca aagtgaagag cggaaaataa tccgcagcct
    46681 tagatgccgg gcgggagcgc cactccctcc ggcaattcgc accaacatgg tgcgcttgca
    46741 cacatttaat gccggagagc agccgtgaat gacgctatcc ctcgccccca ggcgaaaacc
    46801 cgtaaggcgc tgaccccgct attagaaatc cgcaatctga ccaaatccta cgatggtcag
    46861 catgcggttg atgatgtcag cctgaccatc tacaaaggtg aaatctttgc gctgctgggc
    46921 gcatccggct gtggcaagtc cacgctgctg cgtatgctgg caggtttcga acaaccttca
    46981 gccgggcaga ttatgcttga tggcgtcgat ttgtcacagg tgccgcctta cctgcgcccc
    47041 atcaatatga tgtttcagtc ttacgcgctg tttccgcata tgaccgtgga acagaacatc
    47101 gcttttggcc tgaaacagga caaactaccg aaagcggaaa ttgccagccg ggtcaatgag
    47161 atgctcggcc ttgtgcatat gcaggagttc gccaaacgca aaccgcatca gctttccggt
    47221 ggtcagcgac agcgtgtggc actggcccga agccttgcca agcgcccgaa actattactg
    47281 ctcgacgagc cgatgggcgc gctggataaa aagctgcgtg accggatgca gcttgaagtg
    47341 gtggatattc tggaacgcgt cggtgtgact tgtgtgatgg tcacccacga tcaggaagag
    47401 gcgatgacca tggcggggcg tatcgccatt atgaatcgtg ggaaatttgt ccagattggc
    47461 gaaccggaag agatctacga gcatccgact acccgctata gcgccgaatt tattggctcg
    47521 gttaatgtct ttgaaggtgt actcaaagag cgtcaggaag atggtctggt gctttattcg
    47581 ccggggctgg tgcatccact gaaagtcgat gcggatgctt cagtggtcga taacgtgccg
    47641 gtacacgtag cgctgcgtcc ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt
    47701 tgtaacttcg cggtggggga ggtgatacac attgcctatc tcggcgatct ttcggtgtat
    47761 cacgttcgtc tgaaaagtgg gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat
    47821 cgtaaagggt taccgacctg gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt
    47881 gtggtgctga cggtttaagg agcaaagatg agtacacttg aacctgctgc ccagtcgaaa
    47941 ccgccgggcg gatttaagct gtggttgtcg cagttgcaaa tgaagcatgg gcgcaaactg
    48001 gtcattgcgt tgccgtatat ctggttgatc ttgctgtttc tgctgccatt tctgattgtc
    48061 tttaaaataa gcctggcgga gatggcgcgc gctattccac cttataccga actgatggag
    48121 tgggctgacg ggcaactgtc catcaccctt aatttcggta atttcctgca actgaccggc
    48181 gatccgctct atttcgatgc ttatctccag tcgttacagg tggcggcgat ttcgacattt
    48241 tgctgtttac tgatcggcta tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc
    48301 cgtaatatct tattactact ggtgatcctg ccgtcgtgga cctcgtttct gattcgtgtc
    48361 tacgcctgga tgggaatatt aaaaaacaac ggtgtgctga ataattttct gctgtggctg
    48421 ggggttatcg atcaaccgct gaccattctt cataccaatc tggcagttta tattggcatt
    48481 gtttacgcct acgtgccgtt tatggtgctg ccgatttata ccgcgttgat tcgtattgat
    48541 tattcgctgg tggaagcagc gctggatctc ggtgcacgac cgctgaaaac gttctttact
    48601 gtgatcgtgc cgctgactaa aggtgggatt attgccggat cgatgctggt gtttatcccg
    48661 gctgtgggcg agtttgtgat cccggaactg ctcggtggcc cggacagcat catgatcggg
    48721 cgcgtgctat ggcaagagtt tttcaataac cgcgactggc cggtggcctc ggcggtagcg
    48781 atcatcatgt tgctgctgct aattgtgccg ataatgtggt ttcacaaaca ccagcaaaaa
    48841 agcgtgggag aacacggatg aataatttac cggtagttcg ttcgccctgg cggattgtga
    48901 ttttgctgtt gggcttcacc tttctctacg cgccaatgct gatgctggtt atctattcgt
    48961 ttaacagctc gaagctggtg acggtgtggg ccggctggtc aacgcgctgg tatggcgaat
    49021 tattgcgcga tgacgcgatg atgagtgcgg ttggtttaag tctgacaatt gcggcctgtg
    49081 cggcgacggc ggcggcgatc ctcggaacta ttgcggcggt ggtgctggtg cgctttggca
    49141 ggtttcgcgg atcaaatggc tttgccttta tgatcaccgc gccgctggta atgccagatg
    49201 tcatcacggg cttgtcgctg ttgttattat tcgtcgcgct tgctcatgcc attggctggc
    49261 ctgcggaccg cggtatgctt accatctggc tggcgcatgt cactttctgt acggcttatg
    49321 tggcagtcgt tatttcgtcg cgtcttcggg aactggatcg ctcgatagaa gaagcagcga
    49381 tggatctcgg tgcgacgccg ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc
    49441 ccgcggtcat ttctggctgg ttactggctt ttactttgtc gcttgatgat ctggtgatcg
    49501 ccagctttgt atccgggccg ggagccacca cgttaccgat gctggtcttt tccagcgtgc
    49561 ggatgggggt gaatccggaa atcaacgccc tagcaacatt gatcctcggc gcggtcggaa
    49621 ttgtcggatt tatcgcctgg tatctgatgg ctcgcgcaga aaaacagcgg atacgcgaca
    49681 tccagtgtgc aagacgtggc tgaagacact aaaatttgcc aacctggcta cataatgccg
    49741 cgtatgtcgc ggcattgttt tcatggaaga cgaaacgttg ggatttttta agaaaacatc
    49801 ttcatctcat gctcgcctga atgtgcctgc gcttgtgcag gtggcggcgc tcgccattat
    49861 tatgatccgt ggcctcgacg tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg
    49921 cgagttcatt catcgcagcg tacaaacctg gagtttaacg ctggtctttt taagcagtct
    49981 ggtgctggtt ttcattgaga tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc
    50041 gcgctggctg tatctgctga cacaaatcac tgccgcaagt tacttgtggg cggcttcgct
    50101 ggggtacggt tatccggagc tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca
    50161 tagcctgatg ctgcagaagc tgccggatat gctcatcctg atgctgctgt tcgttccctc
    50221 gaccagtcgg cggttcttcc agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg
    50281 aaggtcaatg tatgcagtgc gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga
    50341 taacgcagcc gattccagag caactctccg ctaaaaccgc cgatcttaaa aatctgctcg
    50401 ccgactttcc ggttgaggaa tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta
    50461 ataaagccaa aatggtggtg agtggtagcg ttgaaaaacc actgctcggt atgctgcatc
    50521 gcgacggcac accggaagac ctttgtgact gcccgcttta tcctgcctca tttgcgcccg
    50581 tttttgcggc gctaaaaccg tttatcgccc gagcggggtt aacgccctac aacgtggcgc
    50641 gtaagcgtgg cgaactgaaa tacattctgc tgactgaaag ccagagcgat ggcggcatga
    50701 tgctgcgctt tgtactgcgt tctgaaacca aactagcaca actgcgtaag gcattgccgt
    50761 ggttacaggc acaattgccg cagctaaaag ttatcaccgt caatattcag ccggtacata
    50821 tggcgattat ggaaggggag acggagatct acctgaccga acaacaggcg ctggtagagc
    50881 gttttaacga tgtgccgctg tggatccgtc cgcaaagttt cttccagact aatccggcgg
    50941 tcgccagcca gctgtacgct accgcgcgcg actgggtacg acagctgccg gttaaacata
    51001 tgtgggatct gttctgcggt gtggggggct ttggtttaca ctgcgcgacg cctgacatgc
    51061 agttaaccgg gatcgaaatt gcgctagagg ccattgcctg tgcaaagcag tcagccgctg
    51121 aactgggctt aacgcgtctg caatttcagg cgctggactc cactcagttt gccaccgctc
    51181 agggggaggt gccggagctg gtgctggtta acccgccgcg ccgcggcatt ggtaaaccgc
    51241 tgtgtgatta tctctcaacg atggcaccgc gttttattat ctactccagc tgtaacgccc
    51301 aaaccatggc gaaagatatc cgcgaactgc caggttaccg catcgaacgg gtacagcttt
    51361 ttgatatgtt cccgcacacc gcgcactatg aagtgctgac gctgctggta aaaatgtaaa
    51421 tacatcattc aaatcaatat cacctcggca tacatccatc caaaataata atcgtgatcg
    51481 atcgattaat aataac
//
LOCUS       DAZGXS010000018        50165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGXS010000018 DAZGXS010000000
VERSION     DAZGXS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            49..1149
                     /gene="torY"
                     /locus_tag="ACHKG5_001200"
     CDS             49..1149
                     /gene="torY"
                     /locus_tag="ACHKG5_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0867339.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1174..3603
                     /gene="torZ"
                     /locus_tag="ACHKG5_001201"
     CDS             1174..3603
                     /gene="torZ"
                     /locus_tag="ACHKG5_001201"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0867340.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(3768..4739)
                     /gene="cmoB"
                     /locus_tag="ACHKG5_001202"
     CDS             complement(3768..4739)
                     /gene="cmoB"
                     /locus_tag="ACHKG5_001202"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0867341.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4736..5479)
                     /gene="cmoA"
                     /locus_tag="ACHKG5_001203"
     CDS             complement(4736..5479)
                     /gene="cmoA"
                     /locus_tag="ACHKG5_001203"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0867342.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5520..5915)
                     /gene="yecN"
                     /locus_tag="ACHKG5_001204"
     CDS             complement(5520..5915)
                     /gene="yecN"
                     /locus_tag="ACHKG5_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0867343.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(5968..6786)
                     /gene="yecE"
                     /locus_tag="ACHKG5_001205"
     CDS             complement(5968..6786)
                     /gene="yecE"
                     /locus_tag="ACHKG5_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0867344.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(6783..7349)
                     /gene="yecD"
                     /locus_tag="ACHKG5_001206"
     CDS             complement(6783..7349)
                     /gene="yecD"
                     /locus_tag="ACHKG5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0867345.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            7659..9431
                     /gene="aspS"
                     /locus_tag="ACHKG5_001207"
     CDS             7659..9431
                     /gene="aspS"
                     /locus_tag="ACHKG5_001207"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0867346.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            9549..10001
                     /gene="nudB"
                     /locus_tag="ACHKG5_001208"
     CDS             9549..10001
                     /gene="nudB"
                     /locus_tag="ACHKG5_001208"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0867347.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            10030..10770
                     /gene="yebC"
                     /locus_tag="ACHKG5_001209"
     CDS             10030..10770
                     /gene="yebC"
                     /locus_tag="ACHKG5_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0867348.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            10805..11326
                     /gene="ruvC"
                     /locus_tag="ACHKG5_001210"
     CDS             10805..11326
                     /gene="ruvC"
                     /locus_tag="ACHKG5_001210"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0867349.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(11328..11930)
                     /gene="yebB"
                     /locus_tag="ACHKG5_001211"
     CDS             complement(11328..11930)
                     /gene="yebB"
                     /locus_tag="ACHKG5_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0867350.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            12001..12066
                     /gene="yobI"
                     /locus_tag="ACHKG5_001212"
     CDS             12001..12066
                     /gene="yobI"
                     /locus_tag="ACHKG5_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP0867351.1"
                     /translation="MYIFITHFFTEYVILKYLLPI"
     gene            12205..12816
                     /gene="ruvA"
                     /locus_tag="ACHKG5_001213"
     CDS             12205..12816
                     /gene="ruvA"
                     /locus_tag="ACHKG5_001213"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0867352.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            12825..13835
                     /gene="ruvB"
                     /locus_tag="ACHKG5_001214"
     CDS             12825..13835
                     /gene="ruvB"
                     /locus_tag="ACHKG5_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0867353.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(14081..14866)
                     /gene="znuB"
                     /locus_tag="ACHKG5_001215"
     CDS             complement(14081..14866)
                     /gene="znuB"
                     /locus_tag="ACHKG5_001215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0867354.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(14863..15618)
                     /gene="znuC"
                     /locus_tag="ACHKG5_001216"
     CDS             complement(14863..15618)
                     /gene="znuC"
                     /locus_tag="ACHKG5_001216"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0867355.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            15697..16629
                     /gene="znuA"
                     /locus_tag="ACHKG5_001217"
     CDS             15697..16629
                     /gene="znuA"
                     /locus_tag="ACHKG5_001217"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0867356.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            16645..17967
                     /gene="mepM"
                     /locus_tag="ACHKG5_001218"
     CDS             16645..17967
                     /gene="mepM"
                     /locus_tag="ACHKG5_001218"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0867357.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            18087..19058
                     /gene="lpxM"
                     /locus_tag="ACHKG5_001219"
                     /gene_synonym="msbB"
     CDS             18087..19058
                     /gene="lpxM"
                     /locus_tag="ACHKG5_001219"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0867358.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMQMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(19189..20631)
                     /gene="pyk"
                     /locus_tag="ACHKG5_001220"
     CDS             complement(19189..20631)
                     /gene="pyk"
                     /locus_tag="ACHKG5_001220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0867359.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(20759..21628)
                     /gene="hexR"
                     /locus_tag="ACHKG5_001221"
                     /gene_synonym="yebK"
     CDS             complement(20759..21628)
                     /gene="hexR"
                     /locus_tag="ACHKG5_001221"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0867360.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21966..23441
                     /gene="zwf"
                     /locus_tag="ACHKG5_001222"
     CDS             21966..23441
                     /gene="zwf"
                     /locus_tag="ACHKG5_001222"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0867361.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23676..25487
                     /gene="edd"
                     /locus_tag="ACHKG5_001223"
     CDS             23676..25487
                     /gene="edd"
                     /locus_tag="ACHKG5_001223"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0867362.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            25524..26165
                     /gene="kdgA"
                     /locus_tag="ACHKG5_001224"
                     /gene_synonym="eda"
     CDS             25524..26165
                     /gene="kdgA"
                     /locus_tag="ACHKG5_001224"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0867363.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(26221..27399)
                     /gene="purT"
                     /locus_tag="ACHKG5_001225"
     CDS             complement(26221..27399)
                     /gene="purT"
                     /locus_tag="ACHKG5_001225"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0867364.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            27533..27823
                     /gene="yebG"
                     /locus_tag="ACHKG5_001226"
     CDS             27533..27823
                     /gene="yebG"
                     /locus_tag="ACHKG5_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0867365.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            27890..28246
                     /gene="yebF"
                     /locus_tag="ACHKG5_001227"
     CDS             27890..28246
                     /gene="yebF"
                     /locus_tag="ACHKG5_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0867366.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            28573..29232
                     /gene="yebE"
                     /locus_tag="ACHKG5_001228"
     CDS             28573..29232
                     /gene="yebE"
                     /locus_tag="ACHKG5_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFP0867367.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            29441..31501
                     /gene="ptrB"
                     /locus_tag="ACHKG5_001229"
     CDS             29441..31501
                     /gene="ptrB"
                     /locus_tag="ACHKG5_001229"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0867368.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(31498..32160)
                     /gene="exoX"
                     /locus_tag="ACHKG5_001230"
     CDS             complement(31498..32160)
                     /gene="exoX"
                     /locus_tag="ACHKG5_001230"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0867369.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(32184..32840)
                     /gene="yobB"
                     /locus_tag="ACHKG5_001231"
     CDS             complement(32184..32840)
                     /gene="yobB"
                     /locus_tag="ACHKG5_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0867370.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(32942..33172)
                     /gene="holE"
                     /locus_tag="ACHKG5_001232"
     CDS             complement(32942..33172)
                     /gene="holE"
                     /locus_tag="ACHKG5_001232"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0867371.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            33311..33685
                     /gene="yobA"
                     /locus_tag="ACHKG5_001233"
     CDS             33311..33685
                     /gene="yobA"
                     /locus_tag="ACHKG5_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0867372.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            33689..34561
                     /gene="copD"
                     /locus_tag="ACHKG5_001234"
     CDS             33689..34561
                     /gene="copD"
                     /locus_tag="ACHKG5_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0867373.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            34574..34915
                     /gene="yebY"
                     /locus_tag="ACHKG5_001235"
     CDS             34574..34915
                     /gene="yebY"
                     /locus_tag="ACHKG5_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0867374.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            35311..35967
                     /gene="pphA"
                     /locus_tag="ACHKG5_001236"
     CDS             35311..35967
                     /gene="pphA"
                     /locus_tag="ACHKG5_001236"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0867375.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(35968..36159)
                     /gene="yebW"
                     /locus_tag="ACHKG5_001237"
     CDS             complement(35968..36159)
                     /gene="yebW"
                     /locus_tag="ACHKG5_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0867376.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(36264..36500)
                     /gene="yebV"
                     /locus_tag="ACHKG5_001238"
     CDS             complement(36264..36500)
                     /gene="yebV"
                     /locus_tag="ACHKG5_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0867377.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(36618..38057)
                     /gene="rsmF"
                     /locus_tag="ACHKG5_001239"
     CDS             complement(36618..38057)
                     /gene="rsmF"
                     /locus_tag="ACHKG5_001239"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0867378.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(38138..40771)
                     /gene="yebT"
                     /locus_tag="ACHKG5_001240"
     CDS             complement(38138..40771)
                     /gene="yebT"
                     /locus_tag="ACHKG5_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0867379.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(40740..42023)
                     /gene="yebS"
                     /locus_tag="ACHKG5_001241"
     CDS             complement(40740..42023)
                     /gene="yebS"
                     /locus_tag="ACHKG5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0867380.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            42153..42650
                     /gene="msrC"
                     /locus_tag="ACHKG5_001242"
     CDS             42153..42650
                     /gene="msrC"
                     /locus_tag="ACHKG5_001242"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0867381.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            42747..43445
                     /gene="proQ"
                     /locus_tag="ACHKG5_001243"
     CDS             42747..43445
                     /gene="proQ"
                     /locus_tag="ACHKG5_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0867382.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            43465..45513
                     /gene="prc"
                     /locus_tag="ACHKG5_001244"
     CDS             43465..45513
                     /gene="prc"
                     /locus_tag="ACHKG5_001244"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0867383.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            45705..46586
                     /gene="htpX"
                     /locus_tag="ACHKG5_001245"
     CDS             45705..46586
                     /gene="htpX"
                     /locus_tag="ACHKG5_001245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0867384.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(46632..48005)
                     /gene="yebQ"
                     /locus_tag="ACHKG5_001246"
     CDS             complement(46632..48005)
                     /gene="yebQ"
                     /locus_tag="ACHKG5_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867385.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            48182..48973
                     /gene="kdgR"
                     /locus_tag="ACHKG5_001247"
     CDS             48182..48973
                     /gene="kdgR"
                     /locus_tag="ACHKG5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0867386.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(49117..49356)
                     /gene="yobH"
                     /locus_tag="ACHKG5_001248"
     CDS             complement(49117..49356)
                     /gene="yobH"
                     /locus_tag="ACHKG5_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0867387.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            49516..49659
                     /gene="mgrB"
                     /locus_tag="ACHKG5_001249"
     CDS             49516..49659
                     /gene="mgrB"
                     /locus_tag="ACHKG5_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0867388.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            49734..50021
                     /gene="yebO"
                     /locus_tag="ACHKG5_001250"
     CDS             49734..50021
                     /gene="yebO"
                     /locus_tag="ACHKG5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0867389.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT        12934 a        12317 c        13010 g        11904 t
ORIGIN      
        1 ttatttatac aatgatttcg gtgttctgga atttaattag aggaatctat gcgagggaaa
       61 aaacgcattg ggttattgtt tttgctgata gcggttgtgg ttggtggtgg cgggttcttg
      121 atggcgcaaa aagccttaca taaaacgtcg gatacagcat tttgcctttc ctgccactcg
      181 atgagtaaac cttttgagga atatcaggga actgtccact tttcgaacca gaaagggata
      241 cgtgcggaat gtgccgattg ccatattcca aagtcaggga tggattattt attcgctaaa
      301 ttaaaagcat ctaaagatat ttatcatgaa tttgttagcg gcaaaataga cagtgacgat
      361 aagttcgaag ctcatcgcca ggaaatggcc gaaacagtat ggaaagaatt aaaagcaact
      421 gactctgcaa cgtgccgtag ttgccattct tttgatgcca tggatattgc ctcgcaaagt
      481 gaatctgcgc agaaaatgca taacaaagca caaaagggcg gcgaaacctg tatcgattgt
      541 cataaaggca ttgcccattt tccgccagaa ataaaaatgg atgacaacgc ggcgcatgag
      601 ctggaaagtc aggccgctac ttcagtgact aatggcgcac atatttatcc tttcaaaact
      661 tctcgcatag gcgagctggc taccgtgaat cctggtaccg atctcaccgt cgttgatgcc
      721 agtggcaaac agccgatcgt tctgttgcag ggttatcaaa tgcagggcag tgaaaacacg
      781 ctctacctgg cggcaggtca acggctggcg ctagccacat taagtgaaga aggtatcaag
      841 gcgctcacgg taaacgggga atggcaggct gacgaatacg gcaatcaatg gcgtcaggcg
      901 tctttacagg gtgcgcttac cgatcccgca ttagcggacc gtaaactgct atggcaatac
      961 gctgaaaaac ttgacgatac ctattgcgct ggttgtcatg cccctattgc cgccgaccat
     1021 tacaccgtca atgcgtggcc gtccattgcc aaaggaatgg gggcacgaac cagcatgagc
     1081 gaaaacgaac tggacatttt aacgcggtat ttccagtaca acgccaaaga tattaccgag
     1141 aaacagtgat cctcgttgat cagggaggaa gttatgacat taacaagacg tgaatttatt
     1201 aaacacagcg gtattgccgc tggagcgctg gtggtgacat cggcagcacc gctacccgca
     1261 tgggcagaag agaagggcgg taaaatcctc accgcgggac gttggggagc gatgaacgta
     1321 gaagtgaagg acggcaagat tgtttcttcg acaggcgcgc tggcgaaaac cataccgaac
     1381 tccttacagt ctacggcggc ggatcaggta cacaccacgg cgcgtattca gcatccgatg
     1441 gtgagaaaaa gctatctcga caatccactg caaccggcga aaggtcgtgg cgaagatacc
     1501 tatgtacagg tgagctggga acaggcatta aaactgattc atgagcaaca tgagcgtatt
     1561 cgtaaagcta acggaccatc ggccattttt gccggatctt acggctggcg ctcttctggc
     1621 gtgttgcata aagcgcaaac cttactgcaa cgttatatga acctggcggg cggttattcc
     1681 gggcatagcg gcgattattc aaccggggca gcacaggtga tcatgccgca tgtggtcggt
     1741 tctgtagagg tgtatgaaca gcagacctcc tggccgctga ttctggaaaa cagccaggtt
     1801 gtggtgctgt ggggaatgaa cccgctgaat accttaaaaa ttgcctggag cagtaccgat
     1861 gagcaagggc tggaatactt ccatcagctg aaaaaatctg gcaaaccagt gattgccatt
     1921 gatcctatcc gctctgaaac catcgagttc tttggctata atgccacctg gatcgcgccg
     1981 aatatgggca ccgacgtggc actgatgtta gggattgcgc ataccctgat gacacaaggc
     2041 aaacacgata aagtgttcct tgaaaaatac accaccgggt atccgcagtt tgaagagtat
     2101 ctgacaggta agagcgataa tactccgaag agcgcggcct ggacggcgga aattactggc
     2161 gtaccggaag cgcagatagt caaactcgct gaactgatgg cggcgaatcg cacaatgctg
     2221 atggcaggct ggggaattca gcgccagcaa tatggtgaac agaaacactg gatgctggtg
     2281 acgctggcgg cgatgttagg gcagattggt acaccgggcg gcggttttgg tttctcttat
     2341 cattactcca acggtggcaa cccgacgcgt gttggcggcg tgctgccgga aatgtctgcg
     2401 gcgattgccg gacaggccag cgaggccgct gatgatggcg gaatgacggc gattcctgtt
     2461 gcccgtatcg tcgatgcgct ggaaaatcct ggcggcaaat atcagcataa cggtaaagaa
     2521 caaacgtatc cgaatatcaa aatgatctgg tgggcgggtg gcggtaactt tacccatcac
     2581 caggatacta atcgtctgat caaagcatgg cagaaaccgg agatgatcgt cgtttctgaa
     2641 tgctactgga ccgcagctgc taaacatgca gatatcgtat taccgatcac cacatcgttt
     2701 gagcgcaatg acttgacgat gaccggtgat tacagcaacc agcatattgt accgatgaag
     2761 caggctgtcg ctccgcaatt tgaagcgcga aacgattttg acgtgtttgc cgaacttgcg
     2821 gaattactga aaccaggcgg aaaagagatc tataccgaag gtaaagatga aatggcgtgg
     2881 ctgaaatttt tctatgatgc cgctcagaaa ggtgcccgtg cgcaacgcgt cactatgcca
     2941 atgtttaatg ccttctggca gcaaaataaa ctgatcgaaa tgcgccgcag cgagaagaac
     3001 gaacagtacg ttcgttatgg tgatttccgc gccgatccgg tgaaaaatgc gctgggtacg
     3061 ccaagcggca aaattgagat ttactccaaa acgctggaaa aatttggcta taaggattgc
     3121 ccggcacacc caacctggct tgcgcctgat gagtggaaag gtaccgccga cgagaagcag
     3181 ttacagcttc tgaccgcaca tccggcacac cgtttacaca gtcagcttaa ctatgcgcaa
     3241 ctgcgtaaaa aatatgcggt tgccgatcgt gaaccaatca ctattcacac cgaagatgct
     3301 gctcgctttg gtattgcgaa tggcgatctg gtccgtgtct ggaacaaacg tggtcagatt
     3361 ttgacaggcg ctgtggtgac tgacgggatc aaaaaaggcg tggtatgcgt gcatgaaggt
     3421 gcatggccag atctggaaaa tggcttgtgt aaaaacggca gtgcgaacgt gttaacggcg
     3481 gatatcccca gctcgcagct ggcaaatgcc tgtgccggta actctgcgct ggtgtatatc
     3541 gaaaaatata cgggtaacgc accgaagtta acggcgtttg atcagccagc tgttcaggca
     3601 taagacgtga aaagcgcccg gaaacgggcg ctgaggttat gcaaacataa agctgcctga
     3661 tgcgctatgc ttatcaggcc tacatagtcc ctgcaatata ttgaattagc aaggttttgt
     3721 aggctggata aggcgttcac gccgcatccg gcagtaatac cagaccttta cggcttgcgc
     3781 gcaatcagca ccgcgcgttt aggcgcagga taaccttcaa ctgtcttact cggatcatgt
     3841 gggtcgagga aatcggacag agactcggta accatccatt cagtgcgtcg ctgctcttct
     3901 gtagtggtaa cgctcacatc cgcaatgcgg atatctacaa aaccacactt cttcagccag
     3961 tttttcagcg ccagcgcgga agggatgaaa tagacattac gcatttgcgc gtaacgatcg
     4021 cccggcacca gcaccgtgtt ctcatcgcca tcaataacca gcgtttcaag caccagttca
     4081 ccttcattga ccagttgatc tttcaactgc cagagatgct ccagcggtga acgacgatga
     4141 taaagcacgc ccatcgaaaa gacggtatca aaggctttca gtgccggaag ttgttcaata
     4201 cctaacggta acaaatgcgc gcgctgatca ttacccagta gtttacgcac cgcttcaaac
     4261 tggcacagaa acagttgcgt agggtcgatc cccaccgcga ggtgcgcgcc tgcgccaatc
     4321 atgcgccaca tgtgataacc gctgccgcag ccgacatcca gaatggtgcg tccggttaaa
     4381 tcagaaagat ggggcagaac acgatcccat ttccagtcgg aacgccattc ggtatcgatg
     4441 ttgacgccat acagtgagaa cggccctttg cgccacggca tcaggttgcg catcagcgtt
     4501 tcaatgcgtt taatttgccc tgcgctcagt ggctcttcgc tttctgccgt aacgctatgc
     4561 aataaatcaa gacgatacgg tttaatttca ggtagaaact ccaccgcatt tgaccactgc
     4621 ttaaacaacc cgtgctgctg ctcgcgctgc cagttagcaa tctgcgcggg cagcgtttcg
     4681 agccagtgtg aaagatgatt tttggcaatc agagaataaa agttaccaaa gtcgatcatg
     4741 cagcgtcctc tgcttttaat gccaccagtg aaccaaagtt aaagcactgg aaccacagct
     4801 cgctatgctc aaaaccggct ttatgcaggc gtgctttatg ggtttccacg gaatcggtca
     4861 gcatcacgtt ttccagcatg ctgcgcttct ggctgatctc cagttcgctg taaccgttgg
     4921 cacgtttaaa gtcgtggtgc atattgaaca acagttcacc aactttggca tcttcgaaac
     4981 tgaatttttc cgaaagcacc agcgcgccgc cggggttcag cccttgataa attttatcca
     5041 gtaacgcctg gcgctcggaa ggttccagga attgcagggt aaaattcagc accaccatcg
     5101 atgcgttttc aatggcgata tcgcgaatat caccttcaat gacgtctact ggcgtagggg
     5161 ctttataggc gtcaatatga cgacggcagc gttcaatcat cgccggggag ttgtcgatgg
     5221 caataatttt gcaattatca tgatgaatgt tgcgacgcac cgagagcgtc gccgcgccca
     5281 gagaacaacc cagatcgtaa acctgcgtac caggttgaac gaagcgctcg gctaacatac
     5341 caatcatgga aataatattg gaatagccgg gaacggaacg ctggatcata tccgggaaga
     5401 cttcagctac ccgttcatca aaggtccagt cgcccagtct ggcgataggg gcagaaaata
     5461 gcgtgtcgcg gtgagacata acgtaaaaat ccgggaaaaa gaaagtggcg tattgtgcgc
     5521 taacgcaggg agaaaaccaa ctcccagggc atataccaaa gattcgccag caccatcagc
     5581 aacagcgcac accaggtggc gctcatgccg gaacgtcgcc agcggaacag acggtgatga
     5641 aaaccgtaat aatgcatcag acgaccagca agcaaaacga tgccgcaaat atgcaccatc
     5701 caggtttctg cgccattcat ttccataaac agcatcagca caatcgcgat gggaatatat
     5761 tccaccgcgt taccatgaat gcgaatagcg ctttgcagtt cgctaaaacc accgtcacca
     5821 taggcaacgc ggtactgcat tcgcaggcga acgacatcaa aagagaactt cattaataac
     5881 aacgcactta aaacggcata cagcgcgctt accatacaaa ctccctttaa aatggccgat
     5941 ggcacctgtc tatgataggt ggcaaattca gaaaagagaa gattgctgtg gaatagccgg
     6001 aactgctccg atctctggaa gcgttttacg taagtcttcc cacagggtat gtaccagttc
     6061 cggggcctgg gcaatatctg gcgtatgtaa aaaaagataa ggcgtagtgg tctgatgcca
     6121 ctgcgctaat ttttgtaacc agacctgaaa taattcccgg ttttgcgtca tatcatcact
     6181 accgataaaa cggatcagcg gatttgtcgc cgtcagtaca gcatgtaccg gaactttagg
     6241 tttttttcgt tgagcgtcgc gaatagcttc actgtgtgga cgtgctgcat gaaccgggcg
     6301 gctgtctaaa atcacctgat taacgccgcg ctgatgtaaa ccgcgattaa gcgtttgttc
     6361 ctcttcccct ttggcgaaaa actgtggatg gcggacttcc acaccataat taaattcgcc
     6421 agggagagaa tcgagaaaat gccaaagtgc aggcagctcc cgtgggccga atgtggcagg
     6481 cagttgcagc cagtattgcc caatgcgcgg agccaacggt gacatgcggg tcaaaaattc
     6541 agtcactaaa tcatcgcaat gtcgtaatgc tgcctgatgc gaaatggtcg ccggaaactt
     6601 aaaacagaag cggaagtcat ctgtggtctg ttcacgccag cgcaggacaa cctcgggttt
     6661 cggcagggcg taaagcgtgg tgttgccctc cacgcagtta aagtgacggg catactcttc
     6721 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     6781 aatcataacg cgttgaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     6841 tggttgatac tgttattgtg ctgctcggcg ctagcggcac tacaggcatc ttcggcaatc
     6901 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaactcc gatattggtc
     6961 gagatcccac ataacactat tgtatcgata cctcggcggc gtaattgcaa ctccagatcc
     7021 gtaccgtaaa acgcacccca ctgacgtttg attatttcga tatcactgtc agttgcacct
     7081 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga aggggcatca
     7141 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caacgcgcac cagaaacacg
     7201 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     7261 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccagc
     7321 gcagtggttt tagcattaag ttcaagcatg atgactcctg aaaaaacaaa atcgtgcctc
     7381 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttg tatgagttcg
     7441 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     7501 acgaggcgtt ccagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     7561 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     7621 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     7681 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     7741 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     7801 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     7861 gcattcaggt cacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     7921 cgaccggcga aatcgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     7981 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     8041 acctgcgtcg tccggaaatg gctcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     8101 tgcgccgttt tatggatgac cacggtttcc tcgacatcga aactccgatg ctgaccaaag
     8161 ccacgccgga aggcgcgcgt gactacctgg tgccttctcg tgtgcacaaa ggtaaattct
     8221 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggctttgacc
     8281 gctactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     8341 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     8401 tggaagcgct ggtgcgccat ctgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     8461 cagtaatgac ctttgcggaa gccgaacgcc gttatggttc tgataaaccg gatctgcgta
     8521 acccgatgga actgaccgac gttgctgatc tgctgagatc tgttgagttt gcagtatttg
     8581 caggtccggc gaacgatccg aaaggtcgcg ttgccgctct gcgtgttccg ggtggcgcat
     8641 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     8701 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     8761 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     8821 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     8881 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     8941 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     9001 accatccgtt cacctcaccg aaagacatga cggctgcaga gctgaaagct gcaccggaaa
     9061 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     9121 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     9181 aggaacagcg cgagaaattc ggcttcctgc tcgacgctct gaaatacggt actccgccgc
     9241 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgaccggc accgacaata
     9301 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     9361 cgagctttgc taacccggct gcactggctg agctgagcat tcaggttgtg aagaaggctg
     9421 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcgag gcggcaactg
     9481 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     9541 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctacg
     9601 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga tgaccccgat ttctggcagt
     9661 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     9721 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     9781 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     9841 atacggaatc atggttctgt cttgcgcttc cgcatgagcg gcagatcgtt ttcactgaac
     9901 atctggctta caagtggctt gatgcgcctg ctgcggcggc gctcactaag tcctggagca
     9961 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
    10021 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
    10081 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
    10141 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cagcaattga taaagcactg
    10201 tctaacaaca tgacccgtga cactctgaac cgcgcaattg cacgtggtgt gggcggtgat
    10261 gatgatgcga acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
    10321 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattc
    10381 agcaaatgtg gcggtaacct cggtactgat ggttccgtag cctatctgtt cagcaaaaaa
    10441 ggcgtcatct ccttcgagaa aggcgatgaa gacaccatca tggaagcagc actggaagca
    10501 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg atgtctacac tgcatgggaa
    10561 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
    10621 gtttccatga tcccgtctac caaagctgat atggatgcag aaaccgcacc gaaactgatg
    10681 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtttacca taacggtgaa
    10741 atctctgatg aggtcgcagc gactctctga tgaggcctgc taaacagcaa aacggagacg
    10801 cgtgatggct attattctcg gcattgatcc gggttcgcgc gtgaccggct acggcgtcat
    10861 ccgccaggta ggtaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
    10921 tgatttaccg tctcgtctga aactcatcta tgcgggcgtg acggaaatca tcacccagtt
    10981 ccagcccgat tatttcgcca ttgaacaagt ctttatggca aagaacgctg actcagccct
    11041 gaaactgggc caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg agttgccagt
    11101 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
    11161 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
    11221 tgccgccgat gcgctggcga ttgccatcac ccactgccac gttagtcaga atgcgatgca
    11281 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
    11341 gtcagttccg gctgaggcgt ttccactccc tccgcatgaa tcaacaccaa acctggatga
    11401 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc caggaaccag
    11461 aatttccaga aagtgagttt tgcattcgga ttgctattta acaattctcc aaacgtctct
    11521 atttcaccca ccggaataca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
    11581 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
    11641 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
    11701 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
    11761 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
    11821 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
    11881 gtaaagactt tatctccaat ttcatattca gcagggtaat taatattcac gttgtcattc
    11941 cattgaaata gatacgaata tacgtaatag cataaaaaat ttattttttc aggaggcgca
    12001 atgtatattt ttattacgca tttcttcact gaatatgtaa tattaaaata tttacttcca
    12061 atataacctg tagaataaat tatactgtgc catttttcag ttcatcgaga cacctcgcaa
    12121 gtttttttca tccttcgctg gatatctatc cagcattttt ttatcataca gcattatctt
    12181 tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat cattgaaaaa
    12241 caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca tatgccgatg
    12301 acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac ccactttgtg
    12361 gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagagcg cacgttgttc
    12421 aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg cgctggcgat cctctccgga
    12481 atgtcagcgc agcagttcgt taatgccgtt gagcgtgaag aagtgggggc actggtgaaa
    12541 ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg ttgaaatgaa agaccgattt
    12601 aaaggtttgc atggcgatct ctttacgcca gccgccgacc tggtactcac gtctcctgcc
    12661 agcccggcga ccgacgatgc tgaacaagaa gcggttgccg cgctggtggc gctgggctat
    12721 aaaccacaag aagcaagccg catggtgagc aaaatcgctc gccctgacgc cagcagtgaa
    12781 acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt gaagcagacc
    12841 gtctgatttc tgccggtacc actttgccgg aagatgtagc agatcgcgcc attcgcccta
    12901 aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag attttcatca
    12961 aagcagcgaa actgcgcggc gatgccctcg atcatttgtt gatttttggt cctccggggt
    13021 tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat ttacgcacga
    13081 cttctggtcc ggtgctggaa aaggcgggcg atttggctgc gatgctcact aaccttgaac
    13141 cgcatgacgt gctgtttatt gatgagatcc accgtttatc gccagttgtt gaagaagtgc
    13201 tgtacccggc aatggaagac taccaactgg atatcatgat tggtgaaggt ccggcggcac
    13261 gctccattaa aattgatttg ccgccgttta ccctgattgg tgcaaccacg cgcgcaggtt
    13321 cgctgacatc accgttgcgc gaccgttttg gtattgtgca acgtctggag ttttatcagg
    13381 tgccggatct gcaatatatc gtcagtcgca gcgcacgctt tatggggctt gagatgagtg
    13441 atgacggcgc gctggaagtt gctcgtcgcg ctcgcggtac gccgcgcatt gccaaccgtc
    13501 tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc tcggcagata
    13561 tcgctgctca ggcgctggat atgttgaatg tcgatgctga aggtttcgat tatatggacc
    13621 gcaaattgtt gctggcggta atcgataagt tctttggtgg gccggtaggt ctggataacc
    13681 ttgcggcagc cattggcgaa gaacgtgaaa ccattgagga tgtgctggaa ccttatttga
    13741 ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg cgggcgtgga
    13801 atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct cgcaccgcat
    13861 ccgacattgg cagtgtgcct gatgcgacgc tattcgcgtc ttatcaggca tataccagag
    13921 atagcgaacc gtaggtcgga aaaggcgctc gcgccgcatc cgacattggc agtgtgcctg
    13981 atgcgacgct gttcgcgtct taccaggcct accagagata gcgaaccgta ggtcggataa
    14041 ggcgctcgcg ccgcatccga caaatgtgtt cagcgataga ttagctggcc tgctttttca
    14101 tcatactgat aataaataac agtgccgcac atagcaccac cgacggaccc gccggcgtat
    14161 cgtaaaacgc ggaaaaggtt aaaccgtcag ttactgccac catccccacc aaaacagcga
    14221 caccagccat ctgttccggc gtacgggcaa agcgacgcgc agtagctgca ggaataatca
    14281 gcaacgaagt aataatcaac gcaccgacaa atttcatcgc tacaccaatc gtcaatgccg
    14341 tcaccagcat caacaacaat ttcactcgct gtaatttcac accatcaaca aacgccagat
    14401 ccgggctaat cgtcatcgac agcaaattgc gccattgcca gaacaaaata gccaccacga
    14461 tgaccacgcc aattgcaata gagatgagat cttctggcgt cactgccagt aaatcaccga
    14521 acaggtaagc catcaaatca acacgaatgt tagacatcag actaacgacc accaggccca
    14581 gcgacagggc actgtgcgcc ataatcccta ataacgtgtc gatcgccagc tgtggacgct
    14641 tctccagcca taccagaccg cccgccagca gcagcgtaac ggcaatcacc gcatagaatg
    14701 gattcacgtc cagcaacaaa ccaaacgcga caccaagtaa tgaggcatga gccagcgtat
    14761 caccgaaata agacatacga cgccagacta caaacgaacc cagcggaccc gcggcacagg
    14821 cgagcatgat cccggctaac caaccgggaa ataataattc aatcatgagc gatcatttcc
    14881 ccgacgcaaa acaattcgtc cctgtaaatc gtgacgatga ttatgatgat ggcgatagat
    14941 acccagttgt tcagcaccac gaggaccaaa cattgaaata aactccggat gcagggaaac
    15001 aacttccggt gtgccggaac aacaaatgtg atgattcagg cacagcactt catcggtttt
    15061 tgccatcacc agatgaagat cgtgagaaac cattaaaacg ccacaatcca gttcacgacg
    15121 cagttggtca ataaggtcat ataacgccac ctggccattt acatccacgc cttgagtggg
    15181 ttcatccagc actaataatt gcgggcgatt taacaatgct cgcgctaaca gtacacgctg
    15241 cgtttcgcca ccagagagct tttgcatcgg tgcgttaatc aggtgcccgg cctggacacg
    15301 tttcagtgca ggcaaaatat cttctttatg tgtaccaggg cgtaagcgta aaaaacggtt
    15361 tacggtcagt ggcaacgtgg tgtcgagata cagcttctgc ggtacatagc cgatgcgcag
    15421 ttttccgttg cgcttgataa ccccttcatc gggtgttacc agcccgagca ctacccgtac
    15481 cagtgtcgac ttacctgcgc catttggccc aagtaaagtc aaaatttttc caggtttaag
    15541 ttccagcgac acatcagaga ggacgcggcg ttggccaaaa gaaaccgaga cattttccag
    15601 ggaaaccaga cttgtcatgt taattttagt cttgcagtag tcatgaaatg ttataatatc
    15661 acacttctca tattcattac gatgattggt cgcattatgt tacataaaaa aacgcttctt
    15721 ttcgcagcat tattcgccgc tctctggggc ggtgcaacac aggccgcaga tgccgccgtt
    15781 gtcgcttcgc ttaaacccgt tgggttcatc gcttctgcca ttgctgatgg ggtaacagaa
    15841 acggaggttt tacttcctga cggcgcttca gaacatgatt attcactgcg tccatcggat
    15901 gtaaaacgct tacagaacgc ggacttagtc gtttgggttg gcccggagat ggaagcgttt
    15961 atgcaaaaac cggtaagcaa attaccagaa gcgaagcagg taacgattgc gcagcttgaa
    16021 gatgtgaaac cgctactgat gaaaagtatt aacggcgatg atgatgatca cgaccacgcg
    16081 gaaaaaagtg acgaagatca ccatcacggc gatttcaaca tgcatctttg gctttcccca
    16141 gagatagcgc gggctacagc ggttgcaatc catggaaaat tagtggaact tatgccgcaa
    16201 agtcgagcca aacttgacgc caacctgaag gattttgagg cacaattagc ctcaaccgaa
    16261 acgcaggttg gtaacgagct cgcgccgctc aaggggaaag gttatttcgt ttttcacgat
    16321 gcttacggct atttcgaaaa acagttcgga ctgacaccgc ttggtcattt taccgttaac
    16381 cctgagattc aacctggcgc gcagcgttta catgaaataa gaacacagtt ggttgagcaa
    16441 aaagcaacct gcgtttttgc tgagccacag ttcaggccag cggtcgttga gagcgtcgca
    16501 cgagggacat ccgttcgtat gggaacgttg gatcccttgg ggacgaatat caaactgggt
    16561 aaaacaagct attcagaatt cctgagtcaa ttagccaacc agtatgcgag ctgcctgaaa
    16621 ggagattaat gaggaagtga ttacgtgcaa cagatagccc gctctgtcgc cctggcgttt
    16681 aataatttac cgcgaccaca ccgcgttatg ttggggtcgc tcaccgttct tactctggcc
    16741 gtcgctgtct ggcggcccta tgtttatcac cgcgatgcca cgccaattgt caaaaccatt
    16801 gagctggaac agaacgaaat tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa
    16861 gctgcacaag aagatgaagc cattccccag gacgaactgg atgacaaaat cgccggtgaa
    16921 gcgggcgtgc atgaatatgt tgtttccact ggcgatacgc taagcagcat tctcaatcag
    16981 tatggtattg atatgggtga tatcacccaa ctggctgcgg ccgacaaaga attgcgtaac
    17041 ctgaaaatcg gtcaacaact ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc
    17101 acctgggaag tgtctcgtcg tgaaacccga acctatgacc gtactgccgc taacggtttt
    17161 aaaatgacca gcgaaatgca gcaaggagag tgggttaaca atctgctgaa aggtaccgtc
    17221 ggggggagct ttgttgccag cgccagaaac gccggtttga ccagcgccga agtgagcgca
    17281 gtgattaaag ccatgcagtg gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt
    17341 gcggtattaa tgtctcgaga aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc
    17401 gtacgtttgc gttcagaagg taaagattat tacgctatcc gcgctgaaga tggcaaattc
    17461 tacgatcgta acggtactgg tctggcgaaa ggattcttgc gattcccgac ggcgaaacag
    17521 ttccgtatct cgtctaactt taacccgcgt cgtactaatc cggtgaccgg tcgcgttgca
    17581 ccacacagag gtgttgattt cgccatgccg cagggtacgc cagtgctttc agtgggtgac
    17641 ggtgaagtgg tggttgccaa acgcagtggc gcagcaggtt attatgtggc tattcgtcat
    17701 ggtcgcagct acaccacgcg ttatatgcac ttgcgcaaga ttctggtgaa accggggcag
    17761 aaggtgaaac gtggcgaccg tatcgcgctt tccggtaata ccggacgttc aaccgggccg
    17821 catctgcact atgaagtatg gataaaccag caggccgtaa acccgctgac ggcaaaactg
    17881 ccgcgtaccg aagggctgac cggctccgat cgtcgcgaat tcctggcgca ggccaaagag
    17941 attgtgccgc agctacggtt tgattaatta acatccattc gcagccggta cgcagtcagt
    18001 accggctttt tttatttggt gcggggcaag ttgcgccgct acactatcac cagattgatt
    18061 tttgccttat ccgaaactgg aaaagcatgg aaacgaaaaa aaataatagc gaatacattc
    18121 ctgagtttga taaatccttt cgccacccgc gctactgggg agcatggctg ggcgtagcag
    18181 cgatggcggg tatcgcttta acgccgccaa agttccgtga tcccattctg gcacggctgg
    18241 gacgtattgc cggacgactg ggaaaaagct cacgccgtcg tgcgttaatc aatctgtcgc
    18301 tctgctttcc agaacgtagt gaagctgaac gcgaagcgat tgttgatgag atgtttgcca
    18361 ccgcgccgca agcgatggca atgatggctg agttggcaat acgcgggccg gagaaaattc
    18421 agccgcgcgt tgactggcaa gggctggaga tcatcgaaga gatgcggcgt aataacgaga
    18481 aagttatctt tctagtgccg cacggttggg ccgtcgatat tcctgccatg cagatggcct
    18541 cgcaagggca gaaaatggca gcgatgttcc ataatcaggg caacccggtt tttgattatg
    18601 tctggaacac ggtgcgtcgt cgctttggcg gtcgtctgca tgcgagaaat gacggtatta
    18661 aaccattcat ccagtcggta cgtcaggggt actggggata ttatttaccc gatcaggatc
    18721 atggcccaga gcacagcgaa tttgtggatt tctttgccac ctataaagcg acgttgcccg
    18781 cgattggtcg tttgatgaaa gtgtgccgtg cgcgcgttgt accgctgttt ccgatttatg
    18841 atggcaagac gcatcgtctg acgattcagg tgcgcccacc gatggatgat ctgttagagg
    18901 cggatgatca tacgattgcg cggcggatga atgaagaagt cgagattttt gttggtccgc
    18961 gaccagaaca atacacctgg atactaaaat tgctgaaaac tcgcaaaccg ggcgaaatcc
    19021 agccgtataa gcgcaaagat ctttatccca tcaaataaaa aagcctctcg cgaggagagg
    19081 ccttcgcctg atgataagtt caagtttgct tcagaatatt cgaaatctgt tgaactatca
    19141 ttgaactgta ggccggatgt ggcgttttcg ccgcatccgg caacgtactt actctaccgt
    19201 taaaatacgc gtggtattag tagaacccac ggtactcatc acgtcgccct gggtgacaat
    19261 caccaggtca ccagacatca agtaaccttt atcgcgcagc agattaaccg cttcgctggc
    19321 agctgctacg ccgtcattag cgctatcaaa gtgcaccggc gtaacgccac gatagagagc
    19381 agtcaggttc agcgtacgtt catggcgcga catggcgaaa attggtagac cagagctgat
    19441 acgggaggtc atcagcgcgg tacgacccga ttcggtcatg gtaatgatcg ccgtaacgcc
    19501 tttcaggtgg ttagctgcgt acattgctga catggcaata gcttcttcca cattgtcgaa
    19561 ctgaacgtcc agacggtgtt tagaaacgtt gatgctcggg attttttccg cacccaggca
    19621 aacgcgcgcc atggctgcaa cagtttctga cggatactgc ccagcggcag tttctgcaga
    19681 cagcatcaca gcgtcagtac catccagaac ggcgtttgct acgtccatga cttctgcacg
    19741 cgtcggcatc gggttagtaa tcattgactc catcatctgg gtcgccgtga ttaccgctcg
    19801 gtttagctga cgcgcacgac ggatcaacgc tttctgaatg ccgaccagtt ccgggtcgcc
    19861 aatttccaca ccgaggtcgc cacgtgcaac cattaccacg tcagaggcga ggatgatgtc
    19921 atccattgca tcctggctgc aaacggcttc cgcacgttca accttggcaa caattttcgc
    19981 atcacatcct gcatcgcgtg ccaggcgacg ggcatagttc agatcttcgc cacagcgtgg
    20041 gaaggagaca gccaggtaat ctacgccaat caacgccgca gtcttaatgt ctgctttgtc
    20101 tttttcggtc agcgcttcag ccgacaaacc gccgccaagt ttgttgatac ctttattgtt
    20161 ggagagggga ccaccgacgg tgacttcggt gaacactttc atgccctgaa cttccagtac
    20221 ttttaactgg acgcgaccat cgtccagcag caggatgtca ccaggcacga cgtcagcagg
    20281 caggcctttg tagttgatac cgactttttc tttgtcgcct tcacctttac ccaggttggc
    20341 gtcgagcagg aatttatccc caatattgag gaaaactttg ccttctttaa aggtggatac
    20401 acggattttg ggcccctgga ggtcacccag aatagccaca tgacgcccca gttttgcggc
    20461 aatctcacga actttatccg cgcgcatttt gtgatcttca ggcgagccgt gagaaaagtt
    20521 catacgtaca acgttggcac ccgccgcgat aactttttca agattattat cgcgatctgt
    20581 tgctgggcct aacgtggtaa cgatttttgt tctgcgaagc cttctggaca tgtaatactc
    20641 cgttgactga aacaaccagg tgttgcttga acatgaaatc cgaatgaaat aacgccgcga
    20701 tggcgataaa ataatctcat tcggatgtcg tataagatta gaacagtgac agtcgctttt
    20761 agcgatcgtc acttaaatta agtaactgct tatcaaaacg cgattccttc agcgcttcct
    20821 tgacccgctt caagttatct ctgaattttg caccgcgtcg caaagtaaat cctgtcgcca
    20881 gcacatctat cacggtcagc tgtgcaagtc gagaaaccat gggcatataa atgtcagtat
    20941 cttccggtac gtcgagggta attgccagcg ttgcttcccg ggcgagcggg gtacccgcag
    21001 aggtgagggc aatcaccata gcgtcgtttt cgcgtgccag ctgcgccagc tcgaccagat
    21061 tttttgttct tccggtgtga gaaatcagca ccaccacgtc tccgtcgcta caattcatac
    21121 aactcatgcg ttgcagcaca atatcatcgg agtacaccac cggaacatta aaacgaaaga
    21181 acttattcat cgcatcgtgg gcaacggcgg ctgaagagcc taatccgaaa aaggcgattt
    21241 tttttgcctg agtgagcaag tcgacggcgc ggttgatggc agatttatcc agtgaatgac
    21301 ggacatgatc gagcgttgcc attgcggact caaatatttt ccctgtgtat gattcaacgc
    21361 tgtcatcttc attgacgttg cgattaacat agggagtgcc attcgccaga ctctgtgcca
    21421 gatgaagttt aaaatcagga aaaccgcgcg tgtccatgct gcgacagaaa cgattcaccg
    21481 tcggttcgct aacattggct tccagtgcca gagcagcaat actcgaatgg atcgcgttat
    21541 cgggcgaagc cagaatgacc tcggcaactt tgcgctctga tttgctcaaa tgttccagct
    21601 gagactggat tttttccagc atattcatga tgtaaagaga ctcacgggta atgacgattt
    21661 ccgcactgaa agaaatcgaa atgcagtttt gtcagatatt acgcctgtgt gctgtgttaa
    21721 tgacaaaagc agataaaaaa gttgttattt tttttcataa catgatcagt gtcagatttt
    21781 tacccaatgg aaaacgatga tttttttatc agttttgccg cactttgcgc gcttttaccg
    21841 taatcgcacg ggtggataag cgtttacagt tttcgcaagc tcgtaaaagc agtacagtgc
    21901 accgtaagaa aattacaagt ataccctggc ttaagtaccg ggttagttaa cttaaggaga
    21961 atgacatggc ggtaacgcaa acagcccagg tctgtgactt ggtcattttc ggcgcgaaag
    22021 gcgaccttgc gcgtcgtaaa ttgctgcctt ccctgtatca actggaaaaa gccggtcagc
    22081 tcaacccgga cacccggatt atcggcgtag ggcgtgctga ctgggataaa gcggcatata
    22141 ccaaagttgt ccgcgaggcg ctcgaaactt tcatgaaaga aaccattgat gaaggtttat
    22201 gggacaccct gagcgcacgt ctggattttt gtaatctcga tgtcaatgac actgctgcat
    22261 tcagccgtct cggcgcgatg ctggatcaaa aaaatcgtat caccattaac tactttgcca
    22321 tgccgcccag cacttttggc gcaatttgca aagggcttgg cgaggcaaaa ctgaatgcta
    22381 aaccggcacg cgtagtcatg gagaaaccgc tggggacgtc gctggcgacc tcgcaggaaa
    22441 tcaatgatca ggttggcgaa tacttcgagg agtgccaggt ttaccgtatc gaccactatc
    22501 ttggtaaaga aacggtgctg aacctgttgg cgctgcgttt tgctaactcc ctgtttgtga
    22561 ataactggga caatcgcacc attgatcatg ttgagattac cgtggcagaa gaagtgggga
    22621 tcgaagggcg ctggggctat tttgataaag ccggtcagat gcgcgacatg atccagaacc
    22681 acctgctgca aattctttgc atgattgcga tgtctccgcc gtctgacctg agcgcagaca
    22741 gcatccgcga tgaaaaagtg aaagtactga agtctctgcg ccgcatcgac cgctccaacg
    22801 tacgcgaaaa aaccgtacgc gggcaatata ctgcgggctt cgcccagggc aaaaaagtgc
    22861 cgggatatct ggaagaagag ggcgcgaaca agagcagcaa tacagaaacc ttcgtggcga
    22921 tccgcgtcga cattgataac tggcgctggg ccggtgtgcc attctacctg cgtactggta
    22981 aacgtctgcc gaccaaatgt tctgaagtcg tggtctattt caaaacacct gaactgaatc
    23041 tgtttaaaga gtcgtggcag gatctgccgc agaataaact gactatccgt ctgcaacctg
    23101 atgaaggcgt ggatatccag gtactgaata aagttcctgg ccttgaccac aaacataacc
    23161 tgcaaatcac caagctggat ctgagctatt cagaaacctt taatcagacg catctggcgg
    23221 atgcctatga acgtttgctg ctggaaacca tgcgtggtat tcaggcactg tttgtacgtc
    23281 gcgatgaagt ggaagaagcc tggaaatggg tagactccat tactgaggcg tgggcgatgg
    23341 acaatgatgc gccgaaaccg tatcaggccg gaacctgggg acccgttgcc tcggtggcga
    23401 tgattacccg tgatggtcgt tcctggaatg agtttgagta atatctgcgc ttatccttta
    23461 tggttatttt accggtaaca tgatcttgca cagattgtag aacaattttt acactttcag
    23521 gcctcgtgcg gattcaccca cgaggctttt tttattacac tgactgaaac gtttttgccc
    23581 tatgagctcc ggttacaggc gtttcactca taaatcctct gaatgaaacg cgttgtgaat
    23641 catcctgctc tgacaactca atttcaggag cctttatgaa tccacaattg ttacgcgtaa
    23701 caaatcgaat cattgaacgt tcgcgcgaga ctcgctctgc ttatctcgcc cggatagaac
    23761 aagcgaaaac gtcgaccgtt catcgttcgc agttggcatg cggtaacctg gcacacggtt
    23821 tcgctgcctg ccagccagaa gacaaagcct ctttgaaaag catgttgcgt aacaatatcg
    23881 ccatcatcac ctcctataac gacatgctct ccgctcacca gccttatgaa cactatccag
    23941 aaatcattcg taaagccctg catgaagcga atgcggttgg tcaggttgcg ggcggtgttc
    24001 cggcgatgtg tgatggtgtc acccaggggc aggatggaat ggaattgtcg ctgctaagcc
    24061 gcgaagtgat agcgatgtct gcggctgtcg ggctgtccca taacatgttt gatggtgctc
    24121 tgttcctcgg ggtgtgcgac aagattgtcc cgggtctgac gatggcagcc ctgtcgtttg
    24181 gtcatttgcc tgcggtgttt gtgccgtctg gaccgatggc aagcggtttg ccaaataaag
    24241 aaaaagtgcg tattcgccag ctttatgccg aaggtaaagt ggaccgcatg gccttactgg
    24301 agtcagaagc cgcgtcttac catgcgccgg gaacatgtac tttctacggt actgccaaca
    24361 ccaaccagat ggtggtggag tttatgggga tgcagttgcc aggctcttct tttgttcatc
    24421 cggattctcc gctgcgcgat gctttgaccg ctgccgctgc gcgtcaggtt acacgcatga
    24481 ccggtaatgg taatgaatgg atgccgatcg gtaagatgat cgatgagaaa gtggtggtga
    24541 acggtatcgt tgcactgctg gcgaccggtg gttccactaa ccacaccatg cacctggtgg
    24601 cgatggcgcg cgcggccggt attcagatta actgggatga cttctctgac ctttctgatg
    24661 ttgtaccgct gatggcacgt ctctacccga acggtccggc cgatattaac cacttccagg
    24721 cggcaggtgg cgtaccggtt ctggtgcgtg aactgctcaa agcaggcctg ctgcatgaag
    24781 atgtcaatac ggtggcaggc tttggtctgt ctcgttatac ccttgaacca tggctgaata
    24841 atggtgaact ggactggcgg gaaggggcgg aaaaatcact cgacagcaat gtgatcgctt
    24901 ccttcgaaca acctttctct catcatggtg ggacaaaagt gttaagcggt aacctgggcc
    24961 gtgcggttat gaaaacctct gccgtgccgg ttgagaacca ggtgattgaa gcgccagcgg
    25021 ttgtttttga aagccagcat gacgttatgc cggcctttga agcgggtttg ctggaccgcg
    25081 attgtgtcgt tgttgtccgt catcaggggc caaaagcgaa cggaatgcca gaattacata
    25141 aactcatgcc gccacttggt gtattattgg accggtgttt caaaattgcg ttagttaccg
    25201 atggacgact ctccggcgct tcaggtaaag tgccgtcagc tatccacgta acaccagaag
    25261 cttacgatgg cgggctgctg gcaaaagtgc gcgacgggga catcattcgt gtgaatggac
    25321 agacaggcga actgacgctg ctggtagacg aagcggaatt ggctgctcgc gaaccacaca
    25381 ttcctgacct gagcgcgtca cgcgtgggaa caggacgtga attattcagc gccttgcgtg
    25441 aaaaactgtc cggtgccgaa cagggcgcaa cctgtatcac tttttaagac gacaaatttg
    25501 taatcaggcg agagaaaact ctgatgaaaa actggaaaac aagtgcagaa tcaatcctga
    25561 ccaccggccc ggttgtaccg gttatcgtgg taaaaaaact ggaacacgcg gtgccgatgg
    25621 caaaagcgtt ggttgctggt ggggtgcgcg ttctggaagt gactctgcgt accgagtgtg
    25681 cagttgacgc tatccgtgct atcgccaaag aagtgcctga agcgattgtg ggtgccggta
    25741 cggtgctgaa tccacagcag ctggcagaag tcactgaagc gggtgcacag ttcgcaatta
    25801 gcccgggtct gaccgagccg ctgctgaaag ctgctaccga agggactatt ccgctgattc
    25861 cggggatcag cactgtttcc gaactgatgc tgggtatgga ctacggtttg aaagagttca
    25921 aattcttccc ggctgaagct aacggcggcg tgaaagccct gcaggcgatc gcgggtccgt
    25981 tctcccaggt ccgtttctgc ccgacgggtg gtatttctcc ggctaactac cgtgactacc
    26041 tggcgctgaa aagcgtgctg tgcatcggtg gttcctggct ggttccggca gatgcgctgg
    26101 aagcgggcga ttacgaccgc attactaagc tggcgcgtga agctgtagaa ggcgctaagc
    26161 tgtaaaagtc aaaatgcccg atcgaggatc gggcattttt gtagcgttta tttgccgggt
    26221 ttaaccctgt acttttacct gtccggcggc gtgcttcgcg cgttcaatgg cgtcaacaac
    26281 actctctgca gtagccagtg ccacccccag acgacggctg ccatcaattt ccggcttacc
    26341 aaataaacga atctgcaaat ctgcgcctac ggcattctgc acattatcaa acgtgacatt
    26401 ctgactggtc agttgtggca gaataacggc agaagctgca ggaccatact gacggatccc
    26461 gccaaccgga agtccgagga aggcacgtac atgcagggca aactctgaga gatcttgaga
    26521 aattaacgtc accatcccgg tatcatgtgg acgaggggag acctcactga aaatcacctc
    26581 atcaccacag acaaacagct cgacaccaaa cagaccataa ccgcccagtg ccagcaccac
    26641 tttacgggca atctcctgcg cacgttcaag ggcaagcggg ctcatttgct gtggttgcca
    26701 ggattcacag tagtcgccat cttcctggcg atgacctact ggtgcacaga aatggacgcc
    26761 atccaccgcg ctgacggtta gcagggtaat ttcgaagtca aacttaacga cgccttcaac
    26821 aattacgcgg cccgctccgg cgcgaccgcc ttgctgagcg tattcccatg cctgagcaag
    26881 ttgctctgca gagcgaataa acgtctgccc cttgccggaa gagctcatca ccggttttac
    26941 aatgcaggga tagccaatgg cggcaaccgc ctcgcggaaa aggctttcgc tatcggcaaa
    27001 acgataagtg gaagtgggca gctgcagctc ttctgccgcc aggcgacgga taccctcgcg
    27061 attcatcgtt aatttcgttg cgcgagcgca ggggacaaca ttcagtcctt cctcttcaag
    27121 ttggatcagc atatcggtgg caatagcttc gatctccggc acgatataat gtggtttttc
    27181 cagttcaacc acacggcgta atgcatcacc atcaagcata ttaatgacat gggagcgatg
    27241 cgcgacatgc atggctggtg cgtcggcata gcgatcgacg gcaatcacct ctacgccgag
    27301 acgctgacac tcgattgcca cttctttacc cagttcaccg gagcctaata acatcacgcg
    27361 agttgctgcc ggacgcagcg cagtgcctaa taacgtcata acgaatatcc cctgattaat
    27421 tccgcgcgca gtataaacga aaacgtttgc gtgtgtcttt attgctggtg ttgatctctc
    27481 aaccgaaaag aaatatactg tatagaatca cagttattat gagaggctgc aaatggcggt
    27541 tgaagttaaa tacgtagtca ttcgtgaggg cgaagagaaa atgtcgttta ccagcaaaaa
    27601 ggaagccgat gcttatgaca aaatgcttga tacggcggat ctgcttgaca cctggctgac
    27661 aaattctcca gtgcaaatgg aagacgagca acgtgaagcc ctttcgctat ggctggcaga
    27721 acaaaaagat gtgctgagca ccattctgaa aaccggcaaa ttaccgtccc cacaggtcgt
    27781 cggtgctgaa tccgaagagg aagatgcctc acacgcggcc tgaagcaact tgcgctcctg
    27841 acaatttgta ccatgctttc tcctgaatca ttgtgctatg gagaaaaaca tgaaaaaaag
    27901 aggggcgttt ttagggctgt tgttggtttc tgcctgcgca tcagttttcg ctgccaataa
    27961 tgaaaccagc aagtcggtca ctttcccaaa gtgtgaaggt ctggatgctg ccggaattgc
    28021 cgcgagcgta aaacgtgatt atcaacaaaa tcgcgtggcg cgctgggcag atgatcaaaa
    28081 aattgtcggt caggccgatc ccgtggcatg ggtcagtttg caggacattc agggtaaaga
    28141 tgataaatgg tcagtaccgc taaccgtacg tggtaaaagt gccgatattc attaccaggt
    28201 cagcgtggac tgcaaagcgg gaatggcgga atatcagcgg cgttaatggg ttctgataat
    28261 tcgattgacc gttggcggat gcggcgcgag tgccttgtcc gccccagaac gcaccacaat
    28321 cgtaggcctg agaagacgca acagcgtcgc atcgggcaat cagcatcaaa caatgccact
    28381 caaaacgaca aactactttc acctaaaata cccttatcac ctgatataca gtaaatacct
    28441 tacctggcgc gaatttcttt gaataacggc ctaccgcccc gcttatttgc gcatcttttg
    28501 tctcgatata cattttctga catcctcatc ctctatgcta caggcactaa aaatggttat
    28561 tgaggagcaa atatggctaa ctggttaaat caactgcaat ccctgcttgg gcaaagcagt
    28621 tcttctacct cctcgtctgc ggatcaggga ttgggcaaac ttttagtgcc gggcgcatta
    28681 ggcggactgg ctggactgct ggtcgcaaat aaatcagcac gtaaacttct tacgaaatat
    28741 ggcaccaacg cgttgctgat tggcggcgga gcggtagcgg gtacggtgct gtggaataaa
    28801 tacaaagata aaattcgcgc ggcgcatcag gacgaaccgc agtttggctc gcaaagtacg
    28861 ccgctggatg agcgtacgga acgtttgatc cttgcgctag tctttgccgc taaaagtgat
    28921 ggtcatattg atgccaaaga acgtgcggca atcgaccagc aattgcgtga agccggcgtg
    28981 gaagagcagg ggcgggtact cattgagcag gcaatcgaac aaccgctgga tccacaacgc
    29041 ctggctaccg gggtccgcaa tgaagaagag gcgctggaaa tctatttcct gagctgcgcg
    29101 gctattgata ttgaccattt tatggaacgc agttacctga atgcactggg tgacgcgctg
    29161 aaaatccccc aggatgttcg tgaaggcatt gaacgcgatc tcgaacagca aaaacgcact
    29221 ttggcggaat aattccgctc tctggatagc ctgaagttga ctatttcggg ctatccagaa
    29281 cagcacagcc atttgaacag gctgattttc tctgtctgca taacattcga tattcgcgcc
    29341 attacctgtc atcatctaag caatgactgc cctgtttcgc ttgcatcccc ggtgagtttt
    29401 gccaccctta taagatgttt caaccagaaa gaacaataac atgctaccaa aagccgcccg
    29461 cattccccac gccatgacgc ttcatggcga tacgcgcatc gataattact actggctgcg
    29521 ggacgatacg cgttctcagc cggaagtcct ggactacctg caacaagaaa atagttacgg
    29581 tcatcgggtg atggcctcac aacaagcctt gcaggatcgc atcttaaagg aaatcatcga
    29641 ccgcattccg caacgagaag tttctgcgcc ctacatcaaa aatggctacc gctaccggca
    29701 tatttatgaa ccaggctgtg aatatgctat ctaccagcgt caatcggcgt tcagtgaaga
    29761 gtgggacgag tgggaaatat tgctcgatgc caacaagcgc gcggctcata gtgagtttta
    29821 ttcgatgggc ggaatggcga ttacgcccga taacaccatt atggcgctgg cagaagattt
    29881 tctttcccga cgccagtacg gcattcgttt tcgtaatctg gaaacaggta actggtaccc
    29941 ggaactgctg gataacgttg aacccagctt tgtctgggca aatgactcct ggactttcta
    30001 ctatgttcgc aagcatccgg tgacgctgct gccttatcag gtctggcgtc acgccatcgg
    30061 tacgccagca tcgcaagata aactgattta cgaagaaaaa gacgatacct attacgtcag
    30121 cctgcataaa acgacctcaa agcactatgt agtcattcat ttggccagcg ccaccaccag
    30181 tgaagttcgc ctgctggacg cggaaatggc agatgccgag ccgtttgttt ttctgccgcg
    30241 ccgcaaagat cacgaataca gccttgatca ctaccagcat cgtttttatc tgcgttccaa
    30301 ccgccacggc aaaaactttg gcttataccg tacccgtatg cgtgatgagc aacagtggga
    30361 agagttaatt ccgccacgcg aaaacatcat gctggaaggg tttacgctgt ttaccgactg
    30421 gctggtggtt gaagagcgtc agcgcgggtt aaccagtttg cgccaaatta accgcaagac
    30481 ccgggaagtc attggtattg cctttgatga tccagcctat gtgacctgga ttgcctacaa
    30541 tccagaacct gaaaccgcgc gattgcgtta tggttattct tccatgacca caccagacac
    30601 tttgtttgaa ctggatatgg ataccggtga gcgtcgtgta ttaaaacaaa cagaagttcc
    30661 tggttttgat gcggcgaatt accgcagtga acacctgtgg atagtcgccc gtgatggtgt
    30721 cgaagttccg gtttcgttgg tctaccatcg caatcatttt cgcaaaggac acaacccgct
    30781 gctggtgtat ggctacggtt cttacggcgc aagtattgat gccgatttca gttttagccg
    30841 cttgagtttg ttagatcgtg gctttgtcta cgccattgtc catgttcgcg gcggcggtga
    30901 gctggggcaa caatggtacg aagacggtaa atttctgaag aagaaaaata cgtttaatga
    30961 ttatcttgat gcctgcgatg cattgttaaa actgggctat ggctctcctt cgctttgtta
    31021 tgcgatgggc gggagtgcgg ggggcatgtt gatgggcgtt gcaattaatc aacgcccgga
    31081 attattccac ggcgttatcg cccaggtacc gtttgttgat gttgtaacaa cgatgcttga
    31141 tgaatcaatt cctcttacca ctggtgagtt tgaagagtgg gggaatccgc aggatctgca
    31201 atattacgag tatatgaaaa gctacagccc gtatgacaac gtcaccgcac aggcttatcc
    31261 acatttactg gtaacgaccg gtttacacga ttctcaggtg caatattggg aaccggcaaa
    31321 atgggtcgct aagttgcgcg agctgaaaac cgataaccat cttttattgc tctgtaccga
    31381 catggactca ggccatggcg gtaaatctgg tcgctttaaa tcgtacgaag gcgtagcgat
    31441 ggaatatgct tttctggtcg cgctggcgca gggaacatta cccgctaggc ctgcggatta
    31501 ggtattttcc agataatgtt tcagtgttaa acgcagctcc gggctcatgc tgtccaggtt
    31561 attaaacaac cagcgcagat agcccggatc gcgttcggca acgtcggaaa ccgctttgcc
    31621 acggtatttg ccaaaggtga aggtcgtcat caacgacgga cgtccggtta tatcggccat
    31681 ctgttctgcc gtccagccgg aggtgttcat aatatcgata agcaacgcgg cggtgatata
    31741 acagtcatac aacgcgcggt gatgatgcag gcccggcggc gtctgtacat tgagcttccg
    31801 tgttttatat aacgccatat tgctgtactt gatccctggc cacaaacgac gggccagttt
    31861 catagtgcaa atccactcac cgggcatctc aggcagtacg cggcggtcaa agctggcgtt
    31921 atgcgcgaca taccattcac taccgtaata gtgtgggatc acatcttcaa tccacggttt
    31981 atcggcgacc atggcttcgg tgatgcgatg aatcgccatc gcttgtggac taataggacg
    32041 atcggggtgc accaggtggc tcatggggtt gacgattttc ccgtcaatga catcaacaga
    32101 ggcaatctca acgatccctc cctgcaaacc gcaggtttct gtatcgataa tgcgcaacat
    32161 ggcatgctcc aggccgaaaa agcctagcgt aatggaatga tatcgccttg ccaaccctgt
    32221 gatgaacgct gaccgactaa caataatgaa ccacgatcgg cgcgaacgat gagacgacca
    32281 tgttcatccc atagtgcact gttaccacgg gcatttgcca tcagcacagc aatcgagtac
    32341 tgatgtgaaa aaaattgtaa gcgacgggcc gatgccagca ggtcgggttc tcccagacat
    32401 tgccctgtcg taaacagtga acaggtcggg tccatatcca taccttgcgg ttgttcatcg
    32461 accaccgtga tggttctgga acgacggccc agacatgcac catgactctg atggtagatc
    32521 ccaggcgttt ttcgccacgg tgcgaacacc gcaattccac gaataaagcg atcgttatat
    32581 tcaacgggaa ggccggcaat gatggtcatt cgccaggtcg ttgcggcata acaaagcggg
    32641 tctaacagtg agagatcggg tggggcagga agggcacgtc gcgaataatc acaccccagt
    32701 aaagaaagcg atggaaaaac taacagctga cattgttgcc tggcggcggc cctcacaaac
    32761 tccagatgat gagctacctg ttcggtgagt gaggttttac gaggctcata ttgcgctgct
    32821 gcaactttcc agaacgacat aatgacttcc ttttcaaaat gactgcaaat tatgggtgcg
    32881 gtttagaaaa tactcataat ttagcaggcg ttatgtaaga aagtgtaact ctgtataagt
    32941 tttatttaag tttgggctcg taaggtaaac gtgacagatt gaccgaagcc aaacggtggg
    33001 caataagccg ctcgcgaaac cagctgcgca aatgttcagg ctgttcacgt tcaaccgctt
    33061 cagcgatcac tggcatattg tagcgttctt taaatgccac cccggccgcc gccaaatcga
    33121 cattcacttt atccatttct gtttgatcca gtttagccag attcttcagc attcttaatc
    33181 tcctcatcat tcgcgggcaa aagttactaa gagtgagcct ggatggcaag ccggaattga
    33241 tgatcctcga ctcagacatt agacaaggtt atgctctgta aaaaagttat tacaaaaagg
    33301 aatagataat atggcttcaa ctgcacgctc ccttcgctac gcgctggcaa tcctgaccac
    33361 ttcgttggtg acaccttccg tctgggcaca tgcgcattta acgcatcagt atcctgcggc
    33421 aaacgcgcaa gtgacagctg caccgcaggc aatcacttta aacttctcgg aaggtgttga
    33481 aacaggattc agtggtgcaa aaatcacggg gccaaaaaac gaaaatatta aaacattacc
    33541 ggcgaagcga aatgagcagg accaaaagcg actgattgtt ccactggccg attcgctgaa
    33601 acccggaacc tacaccgttg actggcatgt tgtttcggtg gacgggcata aaacgaaagg
    33661 acactacacc tttagcgtga aataaatcat gctggcgttt acctggatcg cgctgcgatt
    33721 tatccatttc acctcgctga tgctggtctt tggcttcgca atgtacggtg cctggctggc
    33781 acccttaacg attcgtcgct tgctgacgaa gcgttttctg cgtttacaac agcacgctgc
    33841 cgtctggagt ttgatcagtg ccactgcaat gctcgccgtt cagggcgggt tgatgggaac
    33901 gggatggaaa gatgtatttt cgccaaacat ttggcaggct gttttgcaaa cgcagtttgg
    33961 tggcgtctgg ttatggcaaa ttgttctcgc cctcgtaacg ttgatcgtcg cccttatgca
    34021 accgcggaat atgccacgtt tgctgtttat gctcactacc gcgcaattta ttctgctggc
    34081 gggagtgggg catgcgacgc tgaatgaagg ggtaacggcg aaaatccatc agactaatca
    34141 cgcgattcac ctgatttgtg cggcggcgtg gtttggggga ttactgccgg tgctctggtg
    34201 tatgcaactc atcaaaggcc gttggcgaca ccaggctatt caggcgctga tgcgtttttc
    34261 ctggtgcggg cattttgcgg tgatcggcgt actggcaagc ggcgtgctta atgcattgtt
    34321 gattaccgga tttcccccca cgcttacgac ttactggggc caactcttat tgctcaaagc
    34381 catcctggtg atgattatgg tggtcattgc gctggctaat cgttatgttc ttgtaccgcg
    34441 catgaggcag gatgaagatc gtgcggcacc ctggtttgtg tggatgacta aactcgaatg
    34501 ggcgataggt gcggtagtgc tgctgatcat cagcctgctt gcgactctcg aacctttttg
    34561 atggattacg aaaatgatga aaaaaagtat tctggcgttt ctgttactca ccagttctgc
    34621 agcggcgctg gctgcaccgc aggtgattac cgtcagccgt tttgaagtgg gtaaagacaa
    34681 atgggcgttt aatcgcgaag aggtgatgct gacttgccga ccgggtaatg ctttgtatgt
    34741 catcaaccca agtaccctcg tgcagtatcc tttaaacgat gtcgcacaga aggaagttgc
    34801 cagtgggaag actaaagccc aacccatttc ggtgattcag attgatgatc ccaacaatcc
    34861 cggcgaaaaa atgagtctgg caccgtttat agaacgagct gaaaaactct gttaattacc
    34921 taaaatagcc ttttgatttc caataaaaaa accgcctcag ttctttcacc agaacgggcg
    34981 gtttttaaca tttaagctga tgaccaccgc gctttttatt gaccattttg cacgcaaact
    35041 ggaaaacctg gcgtcgtcat ctattcttaa agggcaaggc aactaagcct gcattaatgc
    35101 caacttttag cgcacggctc tctcccaaga gccatttccc tggaccgaat acaggaatcg
    35161 tgttcggtct ctttttatct gttaaaagcc agaagcattt ccttcgctga ctttatagtc
    35221 aaccataaca cacactctac tgtctgagtc cagcgttttt taacattctt gttaagatta
    35281 tgtgatcttt agcgcgggag gaaaatattg atgaaacagc ctgcgcccgt ttatcagaga
    35341 attgcgggtc atcaatggcg acatatctgg ctttctggcg atatacacgg ttgtcttgag
    35401 cagttgcgcc gcaaattatg gcattgtcgt tttgatccgt ggcgagattt acttatctca
    35461 gtgggagacg ttatcgatcg tgggccgcaa agtttacgtt gtctgcagtt actggaacaa
    35521 cattgggttc gtgcggtaag aggcaatcat gaacagatgg cgatggatgc gctggcatcc
    35581 cagcagatgt ctttgtggtt gatgaatggc ggcgactggt ttattgcgct ggcagataat
    35641 caacagaaac aagcgaaaac ggcgctggaa aaatgtcagc atttgccctt tattcttgaa
    35701 gtacacagcc gcaccggcaa acatgttatt gctcatgccg attatccaga tgatgtttat
    35761 gaatggcaaa aggacgttga tttgcatcag gtcttgtgga gccgctcgcg attaagtgaa
    35821 cgccaaaaag ggcagggaat tacaggtgct gatcatttct ggtttggtca tacaccgttg
    35881 cgccatcgcg tggatattgg caacctgcat tatattgata ccggcgctgt ctttgggggc
    35941 gaactgactt tcgtgcaatt gcaataatta aaaatcacca tactcctgtg ctggtcgcca
    36001 gaaaccatct ataaaatcct caatcggaaa acaaccgcca tgacggatcc gttgatcgct
    36061 catagaataa agacactgct gttccgtgtt gtagacatcc acaacaatat cttcacaacc
    36121 gccatccagg tagcaaacaa aaagtaccag cgcgaacatt tcatccccga agtgtggtgc
    36181 cgtaccgtta agtttaggag agattttaca acgggggaat aaccaggaca aataacccgc
    36241 catgccgacg ggttcttttt ggatcaggca agacgcataa tccaggcatc agattgttga
    36301 tcgtaacggg tacggtaaag gacagagtgt tcgccattta gcagggcagg gatactggtt
    36361 tcagcatccc aggcatccag ttgcatacat aaatcggggt ctgatttaca aggaatggtt
    36421 aacgttcgat cacccggcga ttcaccgtgt aattcgccgt cgtcgatttc aaaagcgcct
    36481 atgcgcacac tggttttcat cttcgtgctc tccatttagc tgccggttgt ggtacgacca
    36541 gtccgatcgc accatttttc agcctagttg aggccgccga atgtgccagt caaaaagtgc
    36601 gctttttttg tgcgctgtca tgcgttactg gtaaaaagtt tcccgtcccg caccagttca
    36661 cgcgggtagc tgtttttcag tcgcgaacca atccgtttgg ctaaaccaat cggctgatgc
    36721 tggaaagtaa ccaacacgtc atccgccact ggcgcggctt gcgggtaaac atcgcgcccg
    36781 cgataccact cctccgcttc ttgcggtgtc agttcaaaag cgttctcgtt gtcgggggag
    36841 gcaagggcaa taaccgcttc atgctgccag cgataacctt tgttgtgcgt ttcggcaagt
    36901 ttaatcccca ggcgagaaaa tcggacttta ccgatcaggg cttcaatgcc taccggaaac
    36961 aaccacactt ctttgtcgcg ctgccagagt cgcaggtttc catcccagtt taagccaaca
    37021 cttgcagccg cctgacgaat ttgtgcggct tcgcgatctt tcaccgggct gaacgggaaa
    37081 ttgcccactt tgtattttgg ggtgggtaag gcgggaatgg cctgagtttt acgcagacga
    37141 gcaacgaaga agccttcgca gtcgtaaatt tgtgggaaaa catgcaaaaa gccttcttcg
    37201 gtcagcgctt tgtttgcacc agggaagaga tcgccaagtg gtaaaaactc tactgcgtcg
    37261 gggtaagtct ctttaagcca caggcaaacg gattcgtttt cttcccggtt taaggtacag
    37321 gtcgagtaaa ccagtgtacc gccagagcgt aatgcatgaa aggcactgtc gatcagttcc
    37381 cgttgggtag ctgcgatttc ctgattgctt tctggtgacc aattttttag cgcatcggga
    37441 tctttacgca ccacgccttc gccggagcag ggagcgtcca gcaaaatggc atcgaacatt
    37501 tctggcaatg ccgcaccaaa cacgcggcca tcaaaatgtg tgagcgcaac attactgata
    37561 ccacagcggc tgatattggc atgtaacact tttacccgac tggcggaaaa ctcattggca
    37621 aggattgccc cttcgttatt catccgcgcg gcaatttgcg tcgttttgga gcctggcgca
    37681 gcagcgacat ccatcacccg ctgtggtgca ttaccgtcag caaacaaggc ggcgacgggc
    37741 aacattgaac tggcttcctg aatataaaac aggccactta aatgctcggc ggtactaccc
    37801 aatggcaatg catcttcatc gtcgcgttca atccagaaac cttcttcaca ccacggaatt
    37861 ggcgtaagcg tccagccata aggagcggtt aatcgcagga aatcagcaac ggaggttttc
    37921 agcgtattaa cgcgaatgct gcggcgcaac gggcgctgac aggcggcaag aaaatcatca
    37981 aaagagagcg tcgaaggcat tgcttcgcgc atttgcgtca gaaaggcgtc cgggaaataa
    38041 acggtgtgtt gggccacgag catataccac tggcaaaaaa aacaggcgcg cagtttagca
    38101 taaacgctcc ggcgcaggca cgccggagca gtgggcatta tttgggaagc gcagttcccc
    38161 attcacgcca ctctttcggt tcactttcct gcaacaggaa gtgcttgcct tcctgggctt
    38221 tcggtgccag tggcgtaccc ggaggcgtgg cgaaggcgat gccgccacgg ataaactggt
    38281 taaaggtgcc ggttttcact acgccgcccg tcagaccaaa gtccagactg taacccgatg
    38341 ccaaccagaa gacggaattg ttacgcacca ggtgttgata gcgtttactg atgcgcatcg
    38401 caatcatcac gcgatctgac aatgtcccca gcgtcattcc ggtaaccgta ccgacttcca
    38461 gaccacggaa cagcacaggc gtaccgatgc ctaacgaaca ggcttccggc gcttcaacaa
    38521 taatgcttaa accatccagg taacgcgaat cagtaatggt ggcctcttgt aattcaaagt
    38581 cgcgacgagg attgccacgg cctggttcga cgttgatata cggctggagg atagtatcaa
    38641 gatgctcaac gccagctgcc gaaatttgcg gtgtgaccac tgagaagcgc gtaccaccgc
    38701 gagcaaaggt ctggacatat tccggataga gcaccgcctt tgcctgtact tcattgcgcg
    38761 cggtaatcag atccagcgtc tggatttgcc cgatatcaat accgagatag cgaattggca
    38821 tcccgaccgc cagttttccg gcatcgaaag catgaagcgt aatctgcccg ccaaccgcac
    38881 gggccgctgt ttcggaagca tacagaatac gcttatcgcc tttacgctga ctggcgctgg
    38941 caccgctgag gttatcgaag ctaatggctc cctttaatgc tctggagagt ggggatgcct
    39001 gtacggtcag gccactacca ttcagctgaa ctttcgcccc gccttctgcc cagaacacgc
    39061 tattgttggt cagaaggttg cgatactccg gcttaatatg cagatcgata tcaaacgcgt
    39121 tagctcgcgg acgcacggta ataacttcac caacttcaaa tttacggtac agcactaccg
    39181 atcctgcctg cacatccggc agcgtctctg cactcaaact cacggttgtg gtgggtaaat
    39241 cgctaaggct gttctccagc gctttttcca gattggcata cagtggatag ctggctttca
    39301 tctcaccttt atcgcccggc agaatacgta tcccgccatt gatccattct gaggcgctgg
    39361 caccgagaaa ctcaacgcca tccagcccca ccttcacatc gacacggctg ttgacgacaa
    39421 atttgctatc gccttttacc agttcgcgat gctgaggctc aatggcgacg gtaaaggtaa
    39481 cgcctttgct ggtgagttta cgatcaataa cctggcctac ctgcacgccg tgaagaatga
    39541 gcggctgacc agcatcaata ccgtaacttt ccggtgctgt cagggtcagt gtcagaacat
    39601 caggttcatg cagcagtgct ttttcgcctg gcacaacaac gaactctttg cgtggctcgc
    39661 catcgccagg caccaactca aaggtttttc cggtcagcag ggcgctgaga ttggcatcgc
    39721 taagggataa tttcgggttg cgtaattcga tgcgggtatt ttcccgtaac agcgtaacga
    39781 cgctgggatc aacggtcatt tctccggtga ctttaccacc aggatttaaa tccagtttag
    39841 tcagctgtcc gacttccagc ccctgataca ttaacggcgt cgagtcggcg gttaatccgg
    39901 ccccactcgg cagttccagt tttattatta cgccacgctg gctgtgggcc agatcttcat
    39961 acagaccaaa ggtatcttcc gcctcggcag gtttcgactc ttctggtgaa tcgaaggcaa
    40021 tcgcaccgtt aaccagtgcc gccagacttt ccagtttcac cttcgcgcca ctgatactga
    40081 cgttggcatc aacgcctgaa acgttccaga aacggctacc ttttttcacc aggtcggtaa
    40141 aacgccgctc gatcaggaca tcaatcacca cgccttgttt attgggattg atggcatagt
    40201 cgtagacttt tcccaccggg atcttgcgga aatagaccaa tgaaccgctg ttcagcgaac
    40261 cgagatcggg agcttgcagg tggatcatca gatcgccgtt atccagccga tacttcggtt
    40321 gggtatcgag cgcgacaaag tgatcctgct ctttaccttt acctggcatc atgccgatat
    40381 agttaccacc gacgagggcg tccagcccgg agacacctgc caacgatgct tttggcgtca
    40441 ccagccagaa ctgagtctct tcgcgcagcg catctttcat atcggacttg atgctgacct
    40501 tgacttcaat cttacgaaga tcgtcgctga ggctgatatc ctgcactgtt ccgacttcaa
    40561 cgccctgata acgaacaggc gtacggcccg ggacaatacc atccgccgac ataaagtcga
    40621 tggtgacggt attaccccgg tcctgataac tgtcccaaat cagccaaccg gcaatcatta
    40681 gcgcgatgaa cggcagcagc cagaaaggtg agatacggcg tttattttta atctgcgctt
    40741 cagtcgtcga agcgggcgtt tcctgactca tgtgcatccc aaagtaagcg gctgtccagc
    40801 cattccacag caagaatagt caaaattacc gctgcgccga aataaaacgc agccggtccc
    40861 atagtaaaag cgaggatctg atcgcgatta atcagcgaca tggttaaaga tatgacaaac
    40921 aggtcgagca tcgaccagcg accaatccag gtcaccatcc gcagtaacag aatgcgtgtg
    40981 cgtaaacctt gctggcattt aaaatgaatg ctcaacagta aagtgaacat gacgatcact
    41041 ttagtaaacg gtaccagaat actggcgata aacacgattc ctgcaaccgc aatgttgcta
    41101 cttgccagcg acataattcc ggaaagaatt gtatcttcct gccgtcctcc gttcagataa
    41161 atgatagaaa taggcaacag gttggcaggt aacaataaaa cgattgacgc taacagcgcc
    41221 gcccagcatt tttgcagact atgacggcga cgcaggcgta gcggaatatg acaacgcggg
    41281 cagcgaccac gctgatcagg atagccggta aaatggcacc caagacagac acgaagtttc
    41341 tcgtccctac gcgtagcggg gcgctgcgga taaaatcgct cccacagctc ctcgacatta
    41401 agatgtgaca acgtcaccgt cgttaaaatc accaacgcca caaaagagaa caagccgaca
    41461 cccgcctgga tatgggcata atcctgtacc tttatagaag caacgccaat gccgaccagg
    41521 tagatatcga gcattaccca ctctttaagt cgctcaagca tcagcagtac cgggcgtaga
    41581 ttcattccca gtcggttacc aaaccataaa taagctatgg aggtcaccag aatgagcggg
    41641 gcaccaataa cgcagaaaaa gaccatcgac cccgttatcg catcgccctg tttggtcatt
    41701 tgccagatgc cttgcataac gttggcgtca atacgaatgc ctaacagcca gatatgcaac
    41761 agcggttcgc cccaggcaaa cggcatcagc aacagcatgg tgaaggccat tgccgccagg
    41821 cgcgttagcg accagtcgcg cccgtcacga atttttgcct gacagcgcgg acaataggca
    41881 ctttgatgag aatttatctc gggcaggcta aacagcatgt cacattgcgg gcaacgttgg
    41941 taatcaccac gcggcagttc ctcgccgatt gccctcactg ttatcttttt tgtcggcgta
    42001 atttgtggtg tgttaagagc catgacgtgc caaatgtaat aagaattaac attaatctta
    42061 acccatgatg ggaatgatct tgagcattaa cgcatttaat gcttatttta ataggctggc
    42121 agttccaggt aagacaacca agtgattata taatgaacaa aacagaattt tacgcggatt
    42181 taaatcgcga ctttaacgcg ctgatggcgg gagaaaccag ttttctggca acgcttgcga
    42241 acaccagtgc gttgttatat gagcgtctca ctgacgtaaa ctgggcaggt ttttatttgc
    42301 ttgaggacga tacactggtg ctcggaccat ttcagggcaa aattgcctgt gtccggatac
    42361 ccgtcgggcg tggcgtgtgc ggcactgcgg ttgcccgcaa tcaagtgcag cgtatcgagg
    42421 atgttcatgc gtttgacggg catattgcct gtgatgcggc gagtaattct gaaattgttc
    42481 tgccgctggt ggtgaaaaat cagattattg gtgttctcga catcgatagt accgtcttcg
    42541 gtcgctttac agacgaggac gagcaaggct tacgtcagct tgtggcacag cttgaaaaag
    42601 tgcttgcaac gacggattac aaaaaattct ttgcgagcgt cgcaggataa tcaacggata
    42661 acgtagcatt tactgatagc gtcattataa tgacgcctgt tcatgcctgc gcttgttggc
    42721 tacgtccgtt gtaatcagga aatttcatgg aaaatcaacc taagttgaat agcagtaaag
    42781 aagtaatcgc gtttctggcc gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc
    42841 gtccgctgaa aatcggtatt tttcaggatt tggtcgatcg tgttgccggg gaaatgaacc
    42901 tgagcaaaac gcaattacga tccgctttac gtctctacac ttcgagctgg cgttaccttt
    42961 acggtgttaa acccggcgca acgcgtgtcg atcttgacgg caacccatgc ggtgagctgg
    43021 acgagcaaca tgtagagcat gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg
    43081 cacagcgtgc tgaacagcaa gcgaaaaaac gcgaagctgc cgcagctgct ggtgagaaag
    43141 aagacgcacc gcgtcgcgaa cgcaagccac gtccgaccac gccacgccgc aaagaaggcg
    43201 ctgaacgtaa acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca gcaaaagcac
    43261 ctcgcgaaga acagcacacc ccagtttctg acatttcagc tctgactgtc ggacaagccc
    43321 tgaaggtgaa agcgggtcaa aacgcgatgg atgccaccgt attagaaatc accaaagacg
    43381 gcgtccgcgt ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt
    43441 tctgaaacgg aggccgggcc aggcatgaac atgtttttta ggcttaccgc gttagctggc
    43501 ctgcttgcaa tagcaggcca gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt
    43561 ccggtattaa aggaagagac gcagcatgcg acggtgagtg agcgcgtaac gtcgcgcttc
    43621 acccgttctc attatcgcca gttcgacctc gatcaggcat tttcggccaa aatctttgac
    43681 cgctacctga atctgctcga ttacagccac aacgtgctgc tggcaagcga tgttgaacag
    43741 ttcgcgaaaa agaaaaccga gttaggcgat gaactgcgtt caggcaaact cgacgttttc
    43801 tacgatctct acaatctggc gcaaaagcgt cgttttgaac gttaccaata cgctttgtcg
    43861 gtactggaaa agccgatgga tttcaccggc aacgacactt ataaccttga ccgcagcaaa
    43921 gcgccctggc cgaaaaacga ggctgagttg aacgcgctgt gggacagtaa agtcaaattc
    43981 gacgagttaa gcctgaagct ggcaggaaaa acggataaag aaattcgtga aaccctgact
    44041 cgccgctaca aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg
    44101 ctggcaatga cggcgtttgc gcgtgaaatc gacccgcata ccaactatct ttccccgcgt
    44161 aataccgaac agttcaacac tgaaatgagt ttgtcgctgg aaggtattgg cgcagtgctg
    44221 caaatggatg atgactacac cgttatcaat tcgatggtgg caggtggtcc ggcagcgaag
    44281 agtaaagcta tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg
    44341 gttgacgtga ttggctggcg tcttgatgat gtggttgcct taattaaagg gccgaagggc
    44401 agtaaagttc gtctggaaat tttacctgct ggtaaaggga ccaagacccg cactgtaaca
    44461 ttgacccgtg aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc
    44521 ggtaaagaga aagtcggcgt gctggatatt cctggcttct atgtgggttt gacagacgat
    44581 gtcaaagtgc aactgcagaa actggaaaaa cagaatgtca gcagtgtgat catcgatctg
    44641 cgtagcaatg gtggtggggc gctgaccgaa gcggtatcgc tctcaggtct gtttattcct
    44701 gcgggtccta ttgttcaggt ccgtgataac aacggtaaag ttcgtgaaga cagcgatacc
    44761 gacggacagg ttttctataa aggcccgctg gtggtactgg ttgaccgctt cagtgcttcg
    44821 gcttcagaaa tctttgccgc ggcaatgcag gattacggtc gtgcactggt tgtgggtgaa
    44881 ccgacgtttg gtaaaggcac cgttcagcaa taccgttcat tgaaccgtat ttacgatcag
    44941 atgttacgtc ctgaatggcc agcgctgggt tctgtgcagt acacgatcca gaaattctat
    45001 cgcgttaacg gcggcagtac gcaacgtaaa ggcgtaacgc cagacatcat catgccgacg
    45061 ggtaatgaag aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc
    45121 attgatgccg cgacttatgt gaaatcaggc gatttaacgg cctttgaacc ggagctactg
    45181 aaggaacata atgcgcgtat cgcgaaagat cctgagttcc agaacatcat gaaggatatc
    45241 gcgcgcttca acgctatgaa ggacaagcgc aatatcgttt ctctgaatta cgctgtgcgt
    45301 gagaaagaga ataatgaaga tgatgcgacg cgtttggcgc gtttgaacga acgctttaaa
    45361 cgcgaaggta aaccggagtt gaagaaactg gatgatctac cgaaagatta ccaggagccg
    45421 gatccttatc tggatgagac ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc
    45481 agacccgcgg aacaacccgc acccgtcaag taatatcaat caggcacaag aaattgtgcc
    45541 tgatttttta acagcggcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc
    45601 tttctggtga cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg
    45661 gtatcgcata ttgcgttttg ttaaactgag gtaaaaagaa aagtatgatg cgaatcgcgc
    45721 tcttcctgct aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag
    45781 ggatacagtc gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg
    45841 gttccttcgt ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag
    45901 tgatcgagca accgcgtaac gaaagggaac gttggctggt caatactgta gcaacccagg
    45961 ctcgtcaggc ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaacg
    46021 cttttgcaac cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc
    46081 agaacatgag cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca
    46141 atggtgatat ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta
    46201 tttcccgtat cctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg
    46261 aagagagcaa cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt
    46321 ttggtattct ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccatgctg
    46381 atgccggttc ggcaaaactg gttggtcgcg agaaaatgat tgccgcgctg cagcgcctga
    46441 aaaccagcta tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacggtaagt
    46501 cgaaatcgct cagtgagttg tttatgactc acccgccgct ggataaacgt attgaagctc
    46561 tgcgtacggg tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgctcg
    46621 acgcgctttt tttatgccct ggatcttggc tgagtgatac gtaacccact gatacaggca
    46681 gcaatcactg ccagaatagc cgcagccatc agcgagacgt gtgtaccatt atctccaaac
    46741 tgatttagca tcagcgccac cagcgccgcg ccgctactct gacccagtag acgagccgtt
    46801 cctaacatgc cactggctcc accgctacgt tcgcgaggcg cggaggtaat aatggtgtgg
    46861 ttattgggtg actggaacaa gccaaatcca gcgccacata agatcatcgg ccagataata
    46921 ttgatatccg caggtgacgc gggcagcaga accagggaaa aaagccccgc agccatgatg
    46981 aataatccta aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccaac
    47041 ggagccatca ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca
    47101 ctacgcccga gcacggtttg caggtaaaag ggcagggaaa ccattgccag catttgtgcg
    47161 cagaaagagc aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc
    47221 accggtagca gcggtactgg aagagaaagc tggcggcgaa taaagaaaat accaacgaca
    47281 accattacaa ccagttccgc agcaattaac gtcagcgatt gcccctgagc gaaaccactc
    47341 aacgcagtga taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa
    47401 cggggtttac tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg
    47461 ataataccca acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt
    47521 gctgcagcaa ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc
    47581 atccctctac ccagaaaacg ttgtggatag atcaggcgga taagggcggt attaacgctc
    47641 atcaacgctg caccgccgaa accttgtatg acacgcgcaa gggtgagtat ttgcagcgaa
    47701 tcagaaaggg cgcagaacag tgaagacaac agaaaaacga ccagaccgca tttataaata
    47761 cgtcgatagc caaacatatc acccagaaac gaaaacgaga gcagggagat gacaatggcg
    47821 atttgatagg cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg
    47881 attgttggca gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc
    47941 acaatggtta atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt
    48001 ggcataggaa ttttaaatct ttggtgaata atcaacaggg gtatgattct agcactgata
    48061 ttcacggtgg atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg
    48121 gtgcgaaatt ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata
    48181 aatggctaac gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt
    48241 tggcattttg caggcgctgg gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg
    48301 cgtcatgatg tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta
    48361 tgtggcgcag gaaggggagt cggagaaata ttccctgacc ctgaaattgt ttgaactggg
    48421 cgctcgcgcg ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat
    48481 ctcccgcctg accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta
    48541 cattcacaaa attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa
    48601 tccactgtac agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt
    48661 gaagcaaatt cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac
    48721 agaagcgtta ttaccggttc tggaccaggt gcgcgagcag gggtatggcg aagataacga
    48781 agagcaggaa gaagggctgc gatgcattgc ggttccggta tttgatcgct ttggcgtggt
    48841 cattgccggt ttgagcatct ccttcccgac gttgcgtttc tctgaagagc gtttacagga
    48901 atatgtcgca atgttgcata ccgcagcgcg caaaatttct gcccaaatgg gttatcacga
    48961 ctatccgttc tgatgagagt aagaacctgt cggaatatca aacagacagg ttctttattt
    49021 agcatgagaa aaataaagtt gaaggtggcg ttagattaaa cgcagttgat ataagaatat
    49081 tttactcatg agtgagaatc tggttattta ttacacttaa ccattatcga ccacgatatt
    49141 gcttttgcgt aacagcgggc aatctgttat ccccaaaaaa ccacttttag tgtgcaagta
    49201 ttgtaccgtg ctggtgcctc tggcagtcag ataggtacat tgcaaaccta attctgcggc
    49261 attctctttg cttccaatca aaacgccata tccgctgagt aataatccta tccataccag
    49321 tgctatcagc ataactgtgc gaatgatgaa tcgcattaca acctcttctc ttttttatgt
    49381 tcgcttaatc gtagcggcaa tatgcgctga agcaagcgac tcattccgaa aaagcacgaa
    49441 tatcgacata gttagacgct gtttaactaa cgcatggtag tttaatgaca taaggtaggt
    49501 gaaacggaga ttggagtgaa aaagtttcga tgggtcgttt tggttgtcgt ggtgttggct
    49561 tgcttgctgc tttgggcgca ggtattcaac atgatgtgcg atcaggatgt acaatttttc
    49621 agcggaattt gtgccattaa ccagtttatc ccgtggtgat atcatttttt cagcactaga
    49681 ttttcttcct ctccagcagt ggtagaatag cgcctctact cagaggtggt gaaatgaacg
    49741 aagttgtaaa ttcaggcgtg atgaacattg cgtctttggt tgtatcggtg gtggttcttc
    49801 ttatcgggct catcttgtgg ttttttatta atcgtgccag ttctcggact aacgaacaga
    49861 ttgaactgct tgaggcgttg ctggatcagc aaaaacgtca aaatgcactg ttacgtcgtt
    49921 tgtgcgaagc aaacgaacca gagaaagcag ataaaaagac tattgagagt caaaaatcgg
    49981 ttgaagacga agatattatt cgcctggtcg ccgaacgata atccttcctc aatcttgagt
    50041 aaccccgtcg ttgaagggtt actcaattca tcctgattat acgcactgtc atggtgcaac
    50101 tgcttcaaat taaaactctg gtctgacttc aaataattat catattattt atttgcgtaa
    50161 cggga
//
LOCUS       DAZGXS010000019        49014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGXS010000019 DAZGXS010000000
VERSION     DAZGXS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            360..1577
                     /gene="alaA"
                     /locus_tag="ACHKG5_001251"
     CDS             360..1577
                     /gene="alaA"
                     /locus_tag="ACHKG5_001251"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0867390.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1661..2260
                     /gene="yfbR"
                     /locus_tag="ACHKG5_001252"
     CDS             1661..2260
                     /gene="yfbR"
                     /locus_tag="ACHKG5_001252"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0867391.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2319..4151)
                     /locus_tag="ACHKG5_001253"
     CDS             complement(2319..4151)
                     /locus_tag="ACHKG5_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0867392.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4238..4888)
                     /gene="hxpA"
                     /locus_tag="ACHKG5_001254"
     CDS             complement(4238..4888)
                     /gene="hxpA"
                     /locus_tag="ACHKG5_001254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0867393.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4899..5393)
                     /locus_tag="ACHKG5_001255"
     CDS             complement(4899..5393)
                     /locus_tag="ACHKG5_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0867394.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5476..5931)
                     /gene="yfbV"
                     /locus_tag="ACHKG5_001256"
     CDS             complement(5476..5931)
                     /gene="yfbV"
                     /locus_tag="ACHKG5_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0867395.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6269..7471
                     /gene="ackA"
                     /locus_tag="ACHKG5_001257"
     CDS             6269..7471
                     /gene="ackA"
                     /locus_tag="ACHKG5_001257"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0867396.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7546..9690
                     /gene="pta"
                     /locus_tag="ACHKG5_001258"
     CDS             7546..9690
                     /gene="pta"
                     /locus_tag="ACHKG5_001258"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0867397.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNKVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9880..11400
                     /gene="yfcC"
                     /locus_tag="ACHKG5_001259"
     CDS             9880..11400
                     /gene="yfcC"
                     /locus_tag="ACHKG5_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0867398.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGAPLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11433..11975)
                     /gene="yfcD"
                     /locus_tag="ACHKG5_001260"
     CDS             complement(11433..11975)
                     /gene="yfcD"
                     /locus_tag="ACHKG5_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0867399.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12033..12584)
                     /gene="yfcE"
                     /locus_tag="ACHKG5_001261"
     CDS             complement(12033..12584)
                     /gene="yfcE"
                     /locus_tag="ACHKG5_001261"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0867400.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12640..13284)
                     /gene="yfcF"
                     /locus_tag="ACHKG5_001262"
     CDS             complement(12640..13284)
                     /gene="yfcF"
                     /locus_tag="ACHKG5_001262"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0867401.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13420..14067
                     /gene="yfcG"
                     /locus_tag="ACHKG5_001263"
     CDS             13420..14067
                     /gene="yfcG"
                     /locus_tag="ACHKG5_001263"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0867402.1"
                     /translation="MIDLYFALTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14124..14486
                     /gene="folX"
                     /locus_tag="ACHKG5_001264"
     CDS             14124..14486
                     /gene="folX"
                     /locus_tag="ACHKG5_001264"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0867403.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14507..15400
                     /locus_tag="ACHKG5_001265"
     CDS             14507..15400
                     /locus_tag="ACHKG5_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0867404.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15448..16338)
                     /gene="rpnB"
                     /locus_tag="ACHKG5_001266"
     CDS             complement(15448..16338)
                     /gene="rpnB"
                     /locus_tag="ACHKG5_001266"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0867405.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSEKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16535..17308)
                     /gene="hisP"
                     /locus_tag="ACHKG5_001267"
     CDS             complement(16535..17308)
                     /gene="hisP"
                     /locus_tag="ACHKG5_001267"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0867406.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17316..18032)
                     /locus_tag="ACHKG5_001268"
     CDS             complement(17316..18032)
                     /locus_tag="ACHKG5_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0867407.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18029..18715)
                     /gene="hisQ"
                     /locus_tag="ACHKG5_001269"
     CDS             complement(18029..18715)
                     /gene="hisQ"
                     /locus_tag="ACHKG5_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0867408.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18805..19587)
                     /gene="hisJ"
                     /locus_tag="ACHKG5_001270"
     CDS             complement(18805..19587)
                     /gene="hisJ"
                     /locus_tag="ACHKG5_001270"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0867409.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19808..20590)
                     /gene="argT"
                     /locus_tag="ACHKG5_001271"
     CDS             complement(19808..20590)
                     /gene="argT"
                     /locus_tag="ACHKG5_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0867410.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20856..21425)
                     /gene="ubiX"
                     /locus_tag="ACHKG5_001272"
     CDS             complement(20856..21425)
                     /gene="ubiX"
                     /locus_tag="ACHKG5_001272"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0867411.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21520..23037)
                     /gene="purF"
                     /locus_tag="ACHKG5_001273"
     CDS             complement(21520..23037)
                     /gene="purF"
                     /locus_tag="ACHKG5_001273"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0867412.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23074..23562)
                     /gene="cvpA"
                     /locus_tag="ACHKG5_001274"
     CDS             complement(23074..23562)
                     /gene="cvpA"
                     /locus_tag="ACHKG5_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0867413.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24094..24756)
                     /gene="dedD"
                     /locus_tag="ACHKG5_001275"
     CDS             complement(24094..24756)
                     /gene="dedD"
                     /locus_tag="ACHKG5_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0867414.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24746..26014)
                     /gene="folC"
                     /locus_tag="ACHKG5_001276"
     CDS             complement(24746..26014)
                     /gene="folC"
                     /locus_tag="ACHKG5_001276"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0867415.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26084..26998)
                     /gene="accD"
                     /locus_tag="ACHKG5_001277"
     CDS             complement(26084..26998)
                     /gene="accD"
                     /locus_tag="ACHKG5_001277"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0867416.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27154..27813)
                     /locus_tag="ACHKG5_001278"
     CDS             complement(27154..27813)
                     /locus_tag="ACHKG5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0867417.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27896..28708)
                     /gene="truA"
                     /locus_tag="ACHKG5_001279"
     CDS             complement(27896..28708)
                     /gene="truA"
                     /locus_tag="ACHKG5_001279"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0867418.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28708..29721)
                     /locus_tag="ACHKG5_001280"
     CDS             complement(28708..29721)
                     /locus_tag="ACHKG5_001280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0867419.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29787..30923)
                     /gene="pdxB"
                     /locus_tag="ACHKG5_001281"
     CDS             complement(29787..30923)
                     /gene="pdxB"
                     /locus_tag="ACHKG5_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0867420.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31022..32017
                     /gene="flk"
                     /locus_tag="ACHKG5_001282"
     CDS             31022..32017
                     /gene="flk"
                     /locus_tag="ACHKG5_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0867421.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32014..33192)
                     /locus_tag="ACHKG5_001283"
     CDS             complement(32014..33192)
                     /locus_tag="ACHKG5_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867422.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33467..34687)
                     /gene="fabB"
                     /locus_tag="ACHKG5_001284"
     CDS             complement(33467..34687)
                     /gene="fabB"
                     /locus_tag="ACHKG5_001284"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0867423.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34846..36852
                     /gene="mnmC"
                     /locus_tag="ACHKG5_001285"
     CDS             34846..36852
                     /gene="mnmC"
                     /locus_tag="ACHKG5_001285"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0867424.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36973..37251)
                     /locus_tag="ACHKG5_001286"
     CDS             complement(36973..37251)
                     /locus_tag="ACHKG5_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0867425.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37285..37833)
                     /gene="epmC"
                     /locus_tag="ACHKG5_001287"
     CDS             complement(37285..37833)
                     /gene="epmC"
                     /locus_tag="ACHKG5_001287"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0867426.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37833..38642)
                     /locus_tag="ACHKG5_001288"
     CDS             complement(37833..38642)
                     /locus_tag="ACHKG5_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0867427.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38642..39466)
                     /gene="mepA"
                     /locus_tag="ACHKG5_001289"
     CDS             complement(38642..39466)
                     /gene="mepA"
                     /locus_tag="ACHKG5_001289"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0867428.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39470..40555)
                     /gene="aroC"
                     /locus_tag="ACHKG5_001290"
     CDS             complement(39470..40555)
                     /gene="aroC"
                     /locus_tag="ACHKG5_001290"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0867429.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40590..41522)
                     /gene="prmB"
                     /locus_tag="ACHKG5_001291"
     CDS             complement(40590..41522)
                     /gene="prmB"
                     /locus_tag="ACHKG5_001291"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0867430.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41688..42239
                     /gene="smrB"
                     /locus_tag="ACHKG5_001292"
     CDS             41688..42239
                     /gene="smrB"
                     /locus_tag="ACHKG5_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0867431.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42410..43252)
                     /locus_tag="ACHKG5_001293"
     CDS             complement(42410..43252)
                     /locus_tag="ACHKG5_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0867432.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43254..43775)
                     /locus_tag="ACHKG5_001294"
     CDS             complement(43254..43775)
                     /locus_tag="ACHKG5_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867433.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(43772..44242)
                     /locus_tag="ACHKG5_001295"
     CDS             complement(43772..44242)
                     /locus_tag="ACHKG5_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867434.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(44239..44739)
                     /locus_tag="ACHKG5_001296"
     CDS             complement(44239..44739)
                     /locus_tag="ACHKG5_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867435.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44750..45508)
                     /locus_tag="ACHKG5_001297"
     CDS             complement(44750..45508)
                     /locus_tag="ACHKG5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0867436.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(45531..48170)
                     /locus_tag="ACHKG5_001298"
     CDS             complement(45531..48170)
                     /locus_tag="ACHKG5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0867437.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(48252..48815)
                     /locus_tag="ACHKG5_001299"
     CDS             complement(48252..48815)
                     /locus_tag="ACHKG5_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867438.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQIIYL"
BASE COUNT        12057 a        13192 c        12207 g        11558 t
ORIGIN      
        1 ttgcccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa
       61 tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac aattatctta
      121 cagataaaaa aaccagactt acaattaaga atcagaacaa ttcactatat aacattgcat
      181 gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca caatcgagcc
      241 ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg gggagtacat
      301 tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca
      361 tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc cgtggtccgg
      421 tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg
      481 gcaacccagc cccgttcggt tttgacgcgc cagatgaaat cctcgttgac gtgatacgca
      541 acctgcctac cgctcaaggg tattgcgatt ccaaaggtct ttactccgcg cgtaaagcca
      601 tcatgcagca ctatcaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg
      661 gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac agcggggacg
      721 aaatgttggt tcctgcacca gattacccac tatggaccgc ggcggtttcg ctttccagcg
      781 gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac ctcgatgata
      841 ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat tatcaaccca aataacccaa
      901 ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca cgtcagcata
      961 atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgac gctgagcatc
     1021 actcaattgc gccgctggca cctgacctgc tgaccattac ctttaatgga ctgtcgaaaa
     1081 cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg
     1141 ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc
     1201 ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt gaatttatta
     1261 cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaattgatc aacgatattc
     1321 cgggcgtttc ctgcgtgaaa ccttgtggtg cgctgtacat gttcccgaaa atcgacgcca
     1381 aacgctttaa cattcacgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag
     1441 ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca
     1501 cgctaccgcg tgtcgatgat atcgagctgt cgttgagcaa gttcgcgcgt ttcctttctg
     1561 gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca gtgttatccc
     1621 gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt
     1681 tgcccatctc tcccgcctga aactcattaa ccgctggccg ctcatgcgca acgtgcggac
     1741 ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat
     1801 caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta
     1861 ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat acttcaattc
     1921 gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcaaaaac tggtcgatat
     1981 ggttccggaa gagctgcggg atatctttgc gccgttaatt gacgagcatg catatagcga
     2041 tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga aatgtctgga
     2101 agaactcgcg gcgggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct
     2161 tgaagcgcgc cgcagccagg agatggacta cttcatggaa gtatttgttc ccagcttcca
     2221 tctttcgctc gatgagatta gccaggattc accgctgtaa gcagctggag tctgcgtcgc
     2281 atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg
     2341 atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccag cttcacaaag
     2401 tcactgaagc tgtaatttcc cggacctaaa accaatgtgt taacaggtga agaaaccggt
     2461 gtcataaagg cggcggatgc tgccatcgcc acgaccatcg cgaatggata aggcgacacc
     2521 cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta
     2581 gagataaata gcccaataac cgccgacaag acaaacaaac aacccagcat catatgtggc
     2641 ccgtaaccac cgccaatgtc catcagccct ttcaccgcca gcgcgacacc tcccgttttc
     2701 tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgaatg
     2761 gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag gcaggcgatg
     2821 atagcggcaa caggattagg aatttcatct gtcagcatta acgccaccat tagcaccaga
     2881 cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac ctcttccggt
     2941 aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta
     3001 cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc
     3061 gccacgccat tgcgctttag ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt
     3121 tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc tgccatgccc
     3181 acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc
     3241 tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca
     3301 cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag
     3361 cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc atccagccgt
     3421 tgaccaatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga
     3481 tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg
     3541 gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg
     3601 accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat
     3661 tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc
     3721 ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg catggcaacg
     3781 cttaacacca cgggaataaa gatagcgaca acgccggttg aactcataaa cgcgccaagc
     3841 cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc cgcaactttg
     3901 accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc gccaataata
     3961 aacaaggcgg caatcaggac aacgttagga tcagaaaagc cagaaaatac ttctgggact
     4021 gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc
     4081 actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac ccaaatcaat
     4141 tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg ccataaaaaa
     4201 gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat
     4261 ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca
     4321 ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc gccaggccag
     4381 aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca agccccagca
     4441 gctgcgcgcc taacagatac gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg
     4501 ttacaaacac ctctggtgcg ggaagccccg ctattttatg gcgcgctcgc gctaccggca
     4561 tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga ttgagtaagg
     4621 cgattgcccc cggaagcgcg gtaatacctt cggtttccgt ggcctcgatt tgctccaaac
     4681 gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcagagagg
     4741 tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc
     4801 tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa
     4861 gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat aatttgatta
     4921 atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc
     4981 tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg
     5041 gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg
     5101 gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa
     5161 ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag
     5221 gtttcttctt tcagctcgcc aaactcgcga tccagttcac gcatctgtaa tccataaccc
     5281 cgctcaatga ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc
     5341 atcatcttgt actggttaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac
     5401 tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc
     5461 gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcacg
     5521 cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg
     5581 tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttgaggattg cagggggtaa
     5641 tggcgtgaca gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa
     5701 cagggcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc agcagagagt
     5761 aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt
     5821 ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt aatgctgtcc
     5881 ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct
     5941 caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt
     6001 caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaag
     6061 tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg
     6121 caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga
     6181 tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt
     6241 tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg
     6301 cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc
     6361 tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa tggacggcaa
     6421 taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt
     6481 taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg
     6541 tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca
     6601 gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat
     6661 cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac
     6721 cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt acaacctgta
     6781 caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca
     6841 ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct
     6901 gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg
     6961 cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat
     7021 catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac
     7081 caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga
     7141 caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc
     7201 gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac
     7261 cggtggtatc ggtgaaaatg ccgcgatggt ccgtgaactg tctctgggca aactgggcgt
     7321 gctgggcttt gaagttgatc atgaacgcaa tctggctgca cgtttcggca aatctggttt
     7381 catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat
     7441 cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc
     7501 tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt
     7561 atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt
     7621 gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc
     7681 ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg
     7741 gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat
     7801 gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt
     7861 ctggttgaag gtctggtccc gacacgtaag caccaatttg cccagtctct gaactacgaa
     7921 atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg
     7981 gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc caaaaacacc
     8041 aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact
     8101 cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caaggttgat
     8161 ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt
     8221 gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac
     8281 gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg
     8341 cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac
     8401 gtgctggttg ccgcttgcct ggctgccatg aacggcgtag aaatcggtgc cctgctgctg
     8461 actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc
     8521 ggcctgccgg tatttatggt gaacaccaac acctggcaga cttctctgag cctgcagagc
     8581 ttcaacctgg aagttccagt tgacgatcac gagcgtatcg agaaagttca ggaatacgtt
     8641 gctaactaca tcaacgctga ctggatcgat tctctgactg ccacttctga gcgcagccgt
     8701 cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa
     8761 cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct
     8821 gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca
     8881 gcgtctcagg gtgtagaact gggtgcaggc attgaaatcg ttgatccaga agtggttcgc
     8941 gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt
     9001 gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa
     9061 gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg
     9121 cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg
     9181 ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac cgcagaacag
     9241 ctggcagaaa tcgcgattca gtccgctgat tccgctgcag ccttcggtat cgaaccgcgc
     9301 gttgctatgc tctcctactc taccggtact tctggtgcag gtagcgacgt agaaaaagtt
     9361 cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg
     9421 cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt
     9481 gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac cacctacaaa
     9541 gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaag
     9601 ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg
     9661 actgcgattc agtctgcaca gcagcagtaa cctcgtcatc atccgcagct ttgcgctgcg
     9721 gatatctgaa ccggaaatag tcactatttc cggtttttta ttctcttaat ttgcattaat
     9781 cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc tcactttgca
     9841 tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc
     9901 aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt
     9961 ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag
    10021 tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcctt
    10081 ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat
    10141 gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc gaaatacggg
    10201 acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg
    10261 cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg tgggaatgaa
    10321 attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg
    10381 ggggaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat
    10441 gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt tgccagttcg
    10501 tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc ggtgctttct
    10561 ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct gatctttacc
    10621 atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac
    10681 cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg
    10741 ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt gatcgttaat
    10801 gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc
    10861 atcggcgttg ttttccgcct taacggtatg acggttaata ccatggcttc atccttcacc
    10921 gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg
    10981 ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt taaatactat cctcaacagc
    11041 attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc
    11101 caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt aaccatgccg
    11161 ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggctttc
    11221 cagtttggtg atggcttcag tcacatcatt tacccgacct cagcttcgtt aatggcgacg
    11281 ctcggtgttt gccgggtgga cttccgtaac tggctgaagg tgggcgcgcc cctgcttgga
    11341 ctgctgttta ttatgtccag cgtcgtggtg atcggcgctc agttgatggg ctaccactaa
    11401 aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct
    11461 tcatttttgg cattgcgctt catccacaac gctagcgctt tcagcgagtc tggagtgaac
    11521 tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct
    11581 tcctgtaggg cgaagggacc gtgagagacg cagctgaaca atgcgcccca gacacggcaa
    11641 tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac
    11701 tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg gactacaccg
    11761 ccagcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc
    11821 agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt
    11881 gcccgcattt gctcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc
    11941 acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc
    12001 gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg
    12061 atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc
    12121 gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc
    12181 cgctgttctg ctactggcag atgggtatga ccgtaaacca gcacatcgtt ctggtttaaa
    12241 gcaggcagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc
    12301 agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg
    12361 ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg
    12421 actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg
    12481 ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc
    12541 gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg
    12601 aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt gcttcgctga
    12661 aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgttgcat aatccaccag
    12721 gcattctggc acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt
    12781 atcagcaatg caccattcac caaataaatt cggctgaccc agtactaaca aatgttctgc
    12841 catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc
    12901 ccccgcaaag acaacatctg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcaa
    12961 ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc
    13021 ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa
    13081 ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt
    13141 cggttgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaaca acaggccttt
    13201 ttcctgcaac gccacccaag cggataacac ataaggggag aaaaaatggg catctgacca
    13261 aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac
    13321 tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta
    13381 taattgtggt gttcagatga ttagacaacc ggagctccaa tgatcgatct ctatttcgcc
    13441 ctgacaccca atggccacaa aattacgctg tttctcgaag aagcagggct ggattatcgc
    13501 ttgataaagg tagacctggg gaaaggcggt cagtttcgcc cggaattttt gctcatttcg
    13561 cctaacaaca aaattccggc aattgttgat cattctccag ccgatggcgg cgaaccgcta
    13621 agcctctttg aatctggtgc cattttgttg tatctggctg agaaaacggg gctctttttg
    13681 agtcatgaaa cgcgtgaacg cgccgccaca ttacagtggt tattctggca ggtaggcgga
    13741 ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct
    13801 tacgctattg aacgttatca ggttgaaact cagcgtctgt accatgtact gaacaagcgg
    13861 ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg
    13921 ccgtgggtta atgcctggac tcgccagcga atagacctcg ctatgtatcc ggcagtcaag
    13981 aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa
    14041 ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta
    14101 agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc
    14161 ttcgtttacg tacatttatc ggaattaagg aagaagaaat taacaaccgt caggatattg
    14221 ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg
    14281 atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt
    14341 tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg
    14401 tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat
    14461 ccatgacctt aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat
    14521 caccggaggg acgggattaa ttggtcgcca tttgattcca cgtttgctgg agctgggcca
    14581 tcaaatcacg gtaatgacgc gtaacccgca gaaagccagt tccgttctcg gccctcgggt
    14641 gacactatgg caagggcttg ccgatcaaag caacctcaac ggcattgatg cggtaatcaa
    14701 cctggccgga gaaccgattg ctgataaacg ttggactcac gagcaaaaag agcgtctctg
    14761 ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc
    14821 accgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt
    14881 ggttaccgaa gaggaaccgc cgcataacaa atttacccat aaactctgcg cccgctggga
    14941 agaaattgcc tgtcgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gtaccggcgt
    15001 agtgctggca ccggatggcg gtattctcgg taaaatgctg ccgccgtttc gtcttggcct
    15061 gggcgggccg attggttccg gtcggcagta tctggcctgg attcatatcg atgatatggt
    15121 caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc
    15181 ctacccgata cgcaatgaac aatttgccca tgcgctcggt catgcgctgc atcgcccggc
    15241 tattttgcgc gtccccgcaa ccgccattcg gctgttaatg ggcgaatctt cagtactggt
    15301 attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg
    15361 gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac tgcaaacatc
    15421 cgccagttaa ctcccggtgt tacagggtta gtggctttgc gcgataagat cgtctggtga
    15481 aagtcgggtc agcgtcagga tgacttcgtg gtcaaatcct ttttccagca tctcctgagc
    15541 aatacgcaga gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc
    15601 aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac caataaatgc
    15661 acggaaacgc tgggcatctc ccgtttgtaa tacataatta aacagggctt ttagctgtct
    15721 gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc
    15781 gcgctgacga atatgtttct gaattaactc caacagcgcc attttacgat gttgcataat
    15841 ctcgtcatcc ggcaccacgg taatatccac caacggaaaa gccgatgaat atattttacg
    15901 ggcaatcgca ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct
    15961 gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc ccacatcaag
    16021 atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta attcttccgg
    16081 cttactttgg tgctctatca ctacataaag ataacccact ccctcctgcg ttttctcaga
    16141 ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt ttggttccag
    16201 tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tatcaataaa
    16261 atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga
    16321 agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac gcgacaataa
    16381 ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta
    16441 tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat tccccttgaa
    16501 aatactccgg cagcgttagc caccggagag gcaattattt cagcgaacct ttaaggaact
    16561 gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgcct tcttcctcta
    16621 ttttcccctg atggaggaaa atgacgtgag tagatacatg gcgagcaaag cccatttcgt
    16681 gggtcaccac caccatcgtt tttccctctt ccgccagctg ctgcataatg cgcagcactt
    16741 cacccaccag ctctgggtca agggccgatg tcggttcatc aaacaacaga acttccggtt
    16801 ccattgccag cgcccgcgct atggaaacac gctgttgctg accaccggac aggtgcaccg
    16861 ggtatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttc accgcccgct
    16921 cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca
    16981 gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca
    17041 gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg cgcaccagat
    17101 tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg aagttaatgc
    17161 agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg
    17221 cattcgcttg cagtgacacc cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat
    17281 cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca
    17341 tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt
    17401 aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata
    17461 tcgcgggcta ttttcagcag atccggcacc gtggcagtaa atgccaacgc agtagagtgc
    17521 agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa
    17581 ataatgcagc gatacatttt aaaagtcgag aagccatagg ctctggcggc ttcaatttcc
    17641 ccatgcggca ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta
    17701 agcgtcagcg ccagcacggt acagttcagg ccactgcgga aaaaggcgtt aagaaactcg
    17761 gtccccttaa caatctccag cgtatacatg ccggaataga acaccagcaa ctgaacatac
    17821 agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta
    17881 ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa
    17941 ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc
    18001 ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacagagt
    18061 agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc aggtaaatca
    18121 cgccacagac gatagcgaaa tagaacggtt cccaggtact tttgcctgcc agctgcgtgg
    18181 ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct
    18241 gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatccgcc
    18301 gaaacacttg cccacgagta aaaccgaacg ccctcgccgc ctctatatgt cctttcggca
    18361 ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag
    18421 tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca
    18481 ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaacact aagtccggca
    18541 cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga ttttgcgaga
    18601 gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag ctgatagcca
    18661 gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc
    18721 ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag
    18781 cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga agtacttttt
    18841 cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcctc
    18901 gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc
    18961 atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt tgaggaaacc
    19021 ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga
    19081 ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg cccaatgttc
    19141 attaccgaac gtctcctggg tagtgccctg caatacgcct acccgtttgc ctttcagcga
    19201 ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat cggcagcgta
    19261 cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa gcgatgacat
    19321 gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg gattttcgac
    19381 aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga tatcgaagcc
    19441 aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat
    19501 gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg ccagaaccag
    19561 agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa
    19621 tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc
    19681 gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac
    19741 gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac agcttccctg
    19801 cgacgtatca gtcaccgtag acattaaagt caaaatactt tttcgccatc ttgtcgtagg
    19861 tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat
    19921 ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc
    19981 cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt
    20041 catcctgcag tgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt
    20101 tggcataggc caccacatcc acacctttgc tacgccaggt ctcgttagcg taagcttcct
    20161 gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgagtccagc gttggctgaa
    20221 tcggcgaacc tttggcggca atcagacggg aatctgcggc gtacagcttg tcggagaagg
    20281 caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg tcgatttttt
    20341 tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta catttcacct
    20401 gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccgacaaag tcacctttgg
    20461 catctttcga cgagaacggt gcataggtgg tatcggttcc gatacgtacc gtctccggca
    20521 atgccgcata gctggaagcc gctgcggaaa taccgactaa caaagacaga gcgagaatcg
    20581 acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg
    20641 tgtttgcagg cttgttcatg caggtcttat gccatcttga cgacaacagt aacattcaac
    20701 gttaaatatg ttaataagac gttgcattat tgtcctaaag ttgaagatag caggtatagc
    20761 ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg
    20821 ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag
    20881 agatcttcag gaagggttat cgcaaactgg tcaagaacac gattaaccgt ctgatttatc
    20941 acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca
    21001 ccgatttctg ctgcctgcgt cattaaacgc agatggccta agtgcaatgg tgtttcacgc
    21061 acgcagagca ccagtgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag
    21121 ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg taaaatcacc
    21181 atccccagtg tctggaaaga accggaagag atgctggcgg caatatcgcg cgcatcatgc
    21241 gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc
    21301 tggcgcgccg cctggctcat taccagatgc gtttcgatat ctgtgacatc gcgcagaacc
    21361 tgtaataagc gcacaccata aatcgcgccg ctggcaccgc tgatgcccac aataagtcgt
    21421 ttcataaata gttgccctgt cagacttgca ggcagacttt gcaggatttc ggcgggagtt
    21481 gcaagtcagg gtgccagacc ggcaccctga gcgaaggcat catccttcgt tatgcatttc
    21541 gagattttcc acttcgttct gacgttgcac tgctttggcg tcgtcattac gtaaagtatc
    21601 gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga
    21661 gcattcaaac tgctggatat ccgggttttc agcgcgaacg gcttcgatca gatcgttcag
    21721 atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcta cttcgcgacc
    21781 gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat
    21841 ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat
    21901 aatctgctca gaagtggtgc cacggacgat agagtcgtcg accagcagga cgtttttatc
    21961 gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac gcagctgctg
    22021 acccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg
    22081 tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttccg ggatcgggat
    22141 caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca gcttcgtgcc
    22201 catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg ggcgagcaaa
    22261 gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa
    22321 caactgccct tcttcagtga tgtaaatcgc ttcgccaggc gcgacgtcac gcaggaaatc
    22381 aaagcccagt gtatcgagcg caacgctttc ggaagcgacc atatattctg tgcggttctc
    22441 gtcaatatca cgttttccca gaaccagcgg acgaataccg tttggatcgc ggaaagcaac
    22501 cataccgtgg ccgataatca tcgccacaca ggcatacgcg ccgcggatta agcggtttgt
    22561 agcagcgatg gcggcgaaaa tgttgtcggc ttcaagcggg tagtggcgga agttgtccag
    22621 ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg
    22681 tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc
    22741 aagcgtaatg ccatacggag agttaacgta aaacggctgc gcttcagagg cgctggagct
    22801 gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg
    22861 gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt
    22921 ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc
    22981 atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg
    23041 tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga
    23101 gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggaa tcagttgtga
    23161 tttactccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga agagaatagc
    23221 agcaacaatc aacacaccgc gcaatgcacc gaaacagacg cccagcaccc gatcggtgcc
    23281 tgacaacccc gttttctcca ccagcaggcc tatcacgaag ttcacgatag cgccaacgat
    23341 cagggtggcg ataaacagga ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa
    23401 gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc
    23461 ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga
    23521 aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta
    23581 cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat
    23641 ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt
    23701 atgcctttgc ggcatcggac aatgcgtgtc ggatgcggcg taaacgtctt atccgaccta
    23761 cggttctacc cctgcgtagg cctgataaga cgcaccagcg tcgcatcagg caacggctgt
    23821 cggatgcggc gtgaacgcct tatccgacct acggttctac ccctgcgtag gcctgataag
    23881 acgcaccagc gtcgcatcag gcaacgcctg tcggatgcgg cgtgaatgcc ttatccgacc
    23941 tacggttcta cccctgcgta ggcctgataa gacgcgccag cgtcgcatca ggcaacggct
    24001 gtcggatgcg gcgtaaacgc cttatccgac ctacggttct acccctgcgt aggcctgata
    24061 agacgcgcca gcgtcgcatc aggcaagacc gtattaattc ggcgtatagc ccattaccac
    24121 gccacttaag ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga
    24181 ggcatccggc ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt
    24241 ataaacccga taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt
    24301 tttcagcgcg cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac
    24361 gaccggcttc ggttctggct tcggcgcagg tggtgcttca accttcggct taggcggctc
    24421 caccggtttc ggcttcggtg ggacgaccgg tgcaggttcc ggttcaaact cggtgttatt
    24481 ggctgcaata gtggccggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc
    24541 cgctgcgcct tccggcggtt gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg
    24601 ctcatcgcga tcgcccgctt ttggcaccag cgggatagcc gcaaactcat cctgataatg
    24661 ttttttctgc ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat
    24721 cgtgcccact aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt
    24781 ccatgacatg tgcgaccgtg tggaacgaac cacacaccag cacggtgtct tccgctttag
    24841 cgtccgccat tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca
    24901 aatgctcaag cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc
    24961 agtcatcaac cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca
    25021 taccgataac cgccagcacg cgcccgtttt tcggtagcgt tttcatacgc ccggtgagat
    25081 attccgccgc atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa
    25141 tctggaaacg tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga
    25201 cctccagccc gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa
    25261 gcggcaacgg caggttttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg
    25321 tgacggaata gttccactca acgccccgac gttgtaacag tgcacctttt tcctgcgcca
    25381 catcagcaat ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgaa
    25441 agatgcctgc tttctcgcga ccaatacttt cgcgatctgg ccccagccag tcggtatgat
    25501 ccagcgcaat actggttact accgcgacat cggcgtcgac aatattggtt gcgtccagac
    25561 gaccgcccag ccctacttcc agaatcacca cgtcaagttg tgcctgtttg aacagccaca
    25621 acgccgacag cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa
    25681 tctccgcaaa agaggcggtg tgggccgatt ccggcaattc ctgaccctgc acacgtacgc
    25741 gctcggtata acacaccaga tgaggcgaac tgtagacgcc cactttgtac cctgccgcca
    25801 tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa
    25861 acacaaatgg cgctggtttc agaacgccaa gacgcgccgc gacctggctc acgcgctcaa
    25921 ggccgagatc gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag
    25981 gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag
    26041 aatagcaaaa gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct
    26101 gatccggtac cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca
    26161 gattcatcaa cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga
    26221 tcatgtcgat cgcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt
    26281 tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa gccgattaac gctttcggtt
    26341 cagcgatgtt gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt
    26401 cggtcagcac ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg
    26461 ttttcgccat ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg
    26521 agaagcagat cagcgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg
    26581 cacccacaac agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa
    26641 ccggcattcc atacagagtg cctttcatca ccaccagcgc atctttttcg ccggtttctt
    26701 tctgcgcaga tgccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt
    26761 tcggctcaag ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc
    26821 gattacgcgc tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt
    26881 ccagctcagc gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag
    26941 gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta
    27001 gggacctttc tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc
    27061 atttttgccc ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg
    27121 gataaaaaag tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg
    27181 tttgtgacgg attatttcga tgacgcccgg caaaatagaa accacaataa tcccgacgat
    27241 cagcagctta aggttatcct gaaccatcgg gattgtaccg aagaaatagc ctgcgtaggt
    27301 aaaaagcagt acccacaaca gtgcgccgat cacgttataa gcagcgaaat gacggtacga
    27361 catgtggccc attcccgcaa caaacggggc gaacgttctg acgatcggca caaaacgggc
    27421 gagaataatc gttttgccgc catgtttctc ataaaactga tgggttttat cgagataact
    27481 gcgacggaaa attttcgaat tgggattact gaataacttt tcaccgaaca accgcccgat
    27541 agtgtaattg accgcgtcac ccacaatcgc ggcgatcagc atcagcacca ccatcatatg
    27601 gacgttgaga tcgttggttt ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc
    27661 acccggtaaa aacggcgtta ccaccagacc ggtttcacag aacaaaatta agaacaaaat
    27721 ggcataaacc cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag
    27781 gataaaatcg atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc
    27841 tttcaggctg taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt
    27901 ccgccagaaa tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca
    27961 ccgcgaccag atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg
    28021 ccgccagcaa ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc
    28081 tgccgacaat attcctgacc atatgatgta caaaggcatt cgctttgata tcaaccacca
    28141 cataaggacc gtgacgcgtg acattaatat gcataacgtt gcgccacggg gttcgggact
    28201 ggcactgcac cgcacggaac gaggtgaaat cattctcgcc cagcaagcac tgcgcagccc
    28261 gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg
    28321 ccgggcgcag ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc
    28381 gggcatgaaa atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat
    28441 tcgcatttac gcccaacgtc cacgcagcgt ctttgcgctg cgcggtagtt tcgaaatgca
    28501 caacctgccc ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg
    28561 gttcgttcgc cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat
    28621 tctgccgttg ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat
    28681 aaactggcgg ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc
    28741 gatttttact gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg
    28801 gacttgctcc ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc
    28861 cgaagcatca cctacctgag ttgggaattc gttctcttca gagagcacaa tatcttcccc
    28921 ttgaacaaac gcatcacgcg cttcttctgc tgccagtgga cgcagagctt caaagttgac
    28981 catctgggca tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagtcc
    29041 ttcgtcctgc aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc
    29101 ttcgctatcc ggcagtaacg gcagcatgtt gaacgcgagc tgacgcccga agaaatcttc
    29161 ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc
    29221 ttttttgccc tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc
    29281 ctgatcgatt aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc
    29341 gatgacattc cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag
    29401 cggtacgtcg ggttcaagag caaacaatcc gctgctgtcg atcaccaggc aacctgagtt
    29461 ggtcgcttct tcaacccagg ctgcggtagc ttctttgcct gcgacaaaaa atgccagctg
    29521 cgcctgcgtc cagtcgaatt cagcagcatc ctgcacggtg attgtcttac caccaaagcg
    29581 caattgttcg cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg
    29641 acgttcagcc agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc
    29701 aatgttccag ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg
    29761 ttatagcaga gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc
    29821 agtttacaca gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat
    29881 tcacggcgct caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact
    29941 ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc
    30001 gtcggttgat cgagcggtcc atgcagagta atgcgaccaa actctggcgc aggcagtaat
    30061 gtatccagcg caacgtgctg ttcatgccca ataaacttgc tataggcttc aaacacttgc
    30121 gtagtaccgc gtgctttacc ttccagggta tagcctgcaa tatgcggcgt gccgatatcc
    30181 acttttgtca gcaattccac atttagttcc ggttcgcctt cccagacatc cagcactacg
    30241 cttaacttct ggccttcatt caggcaggtc agcagcgcgg tattatcgac gactgcgcca
    30301 cggcaggcgt taatcagaat cgctccgggc ttcaggctac ggatcagttt ttcatccgcc
    30361 agatgcagcg ttttgtacgg cccgtctttg aagagtggcg tatggaaagt cagaatatcc
    30421 gcgcgctgga ctaactcatc cagcgagcgg aaatcaccct catccccaca gtcggcgcga
    30481 ggcggatcgc aaagtaaggt tttaatccct aacgcttcca gtcgtgcctg taaacgccga
    30541 ccaacgttac caacgcctac gatccccacg gtacgctcgt gcagtgaaaa tccatcgcgt
    30601 tcggcaagca tcagcaggga ggagaaaaca tattccacca ccgcaatcgc attacagcca
    30661 ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc
    30721 cccgcagtgg cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact
    30781 ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac ggggattgga
    30841 cgcccgggaa ccgcgatcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata
    30901 ttttcatcaa caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac
    30961 aagtgtgcca taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt
    31021 aatgatacaa cctatttccg gccctcctcc tgggcaacca ccaggtcagg gagataacct
    31081 gccgtctggc gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt
    31141 aatgacgaaa gtgacctcac tgacgcaaca gcaaagagca gaactgtggg cgggtatcag
    31201 gcacgatatt ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga
    31261 gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt
    31321 agcgcaatta ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat
    31381 ccgtcataac tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat
    31441 tctcaccctg ttgcaggaag ggaagatggt tattccgcaa ccgcagcagc gcgaggcgac
    31501 cgatcgtcct ctattaccgg cggagcacaa tgcgctcaaa cagctggtga ccaaacttgc
    31561 ggcggcaacg ggggagccca gcaaacagat ctggcaatcg atgctggaac tttccggggt
    31621 gaaagatggc gagttaattc cagcgaaact gtttaaccat ctggtgacct ggctgcaggc
    31681 gcgtcagacg ctaagccagc aaaatacgcc gacgctggaa tcactacaaa tgacgctaaa
    31741 acaaccttta gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg
    31801 cctttctgcg caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta
    31861 tcaacggcgg gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc
    31921 accgatgatg gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt
    31981 cgcgattatc ctgatgctgg tctggctggt tcgttaaccc cgacgaaacg acagtatcgt
    32041 gacaataatt cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa
    32101 cgtggtcgcc agcattcccg caagcggccc ggagacgccg agggcgatat cctgaaacgc
    32161 ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac
    32221 gcccagcgca ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc
    32281 gacccacgcc cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga
    32341 gacaatcgcc actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat
    32401 cacgacaaat gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct
    32461 ggcaaagtag agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag
    32521 acctaaccct ggtttccaga taagtccgac aacgctccac agagatggac gttctcccgc
    32581 cagggctggt actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaatgccat
    32641 tgtggtgagc gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggagc
    32701 accaacagcg agggcaccgt aaatcgccat tccattccat gacatcactt tgccagagtg
    32761 ttttggccct acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc
    32821 aaacccaaga atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg
    32881 caaaatcgcc gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag
    32941 cgccgaacgt tttgcgccat attgatcggc cagtcgcccg gcatagccac gcgtcagcac
    33001 cgtagccaga aactgaatgc cgacggcaat gccgaccatg gtattgccat agcccagatc
    33061 atgatgaaca aacaacggga taaccggcaa cggcaaccct acggtcatgt aggtgagaaa
    33121 aaccgcaaaa gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct
    33181 tacagcagtc atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc
    33241 tctctggttt cagggttaca gtgcgttggc aggatttaac gcgtacgtct tttcagaagg
    33301 aaatcgacaa agcggggagt ttgcctggaa ctggcggcga ttgtcaatga tgtgaaaaag
    33361 ggaaccatca ggttcccttt tgcgttagtg ccggaggccg cattggcgcg taacgtcgga
    33421 tgcgacgctg gcgcgtctta tccgacctac tccggcctac tgcgaattaa tctttcagct
    33481 tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac ataacagtgg
    33541 tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc cgcagcctgc tcgtccagct
    33601 cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga gagtagatag
    33661 cttcctgtac gccagcagcg cccagagagt gaccggtcat ggctttggtt gcagaaatcg
    33721 ccgggctctt atcgccgaac acttcacgga tagctgccag ctctttcacg tcgccaaccg
    33781 gagtcgaagt gccgtgggag ttcaggtaat cgattggggt atcaacgcca tgcatcgcca
    33841 tctgcataca acgtactgcg ccttcgccag agggagcaac catgtctgcg ccatcagacg
    33901 ttgcgccgta gccaacgatt tcagcataga tgtgagcacc acgcgccagc gcgtgttcca
    33961 gctcttcaac caccaccata ccgccgccgc cagcgataac gaaaccgtca cggtgagcat
    34021 cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagacagc gcacccattg
    34081 cgtcgaattc gcaagccatt tcccagcaca gctcttcgcc gccgccagca aacacgatgt
    34141 cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg gaagtcgcac
    34201 acgcggagct gatggagtag ttaacgccat gaattttaaa cggcgtggcg aggcaggcag
    34261 aaacgccgga tgccatcgct ttggtgacca catacgggcc aaccgctttc aggccgcgcg
    34321 ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg ggaaccgccg ccagaacctg
    34381 caatcaggcc aacacgcgga ttattctggt aagcttccgg agagaggccc gcatctgcga
    34441 ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag cgcacaactt
    34501 tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg ctacgcatgc
    34561 cggaatcctt cagctcctga gagaaagtga tccctgaacg tccttcacgc agagatgcca
    34621 ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta atcactgcac
    34681 gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca caatagcgta
    34741 cacttgtacg ccgaacaagt ccgatcagcc aattaataga gaaatttgcg cagccttaca
    34801 cacatcgcta agatcgagct accgcctgta agacgagtaa cttacgtgaa acactactcc
    34861 atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg agattttgac
    34921 gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt tctgggaggt
    34981 aaccaattag aggcacgctt tcctgaacat ccacatcctc tgtttgtggt agcagagagc
    35041 ggcttcggca ccggattaaa cttcctgacg ctatggcagg catttgatca gtttcgcgaa
    35101 gcgcatccgc aagcgcaatt acaacgctta catttcatta gttttgagaa atttcccctc
    35161 acccgtgcgg atttagcctt agcgcatcaa cactggccgg aactggctcc gtgggcagaa
    35221 caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt acttgatgaa
    35281 ggccgcgtga cgctggattt atggtttggc gatattaatg aactgatcag ccaactggac
    35341 gattctctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc agcgaaaaac
    35401 ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg tccaggcggc
    35461 acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga agccggattc
    35521 acgatgcaaa aacgtaaggg ctttggacgc aaacgggaaa tgctttgtgg ggtgatggaa
    35581 cagacattac cactcccctg ctccacgccg tggtttaatc gtacgggcag cagcaaacgg
    35641 gaagtagcga ttatcggcgg tggtattgcc agcgcattgt tgtcgctggc gctattgcgt
    35701 cgcggctggc aagtaacgct ttattgcgcg gatgaagccc ccgcgctggg tgcttccggc
    35761 aatcgccagg gggcgttgta tccgttatta agcaaacacg atgaggcgct aaaccgcttt
    35821 ttctctaatg ggtttacttt tgctcgtcga ctttatgatt cattacccgt taaatttgat
    35881 catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca taaaatcgca
    35941 cagatgttgt caatggattt acccgaagaa ctggctgtag ccgttgaggc aaatgcggtt
    36001 gaacaaatta cgggcgttac gacaaattgc agcggtatta cttatccgca aggtggctgg
    36061 ctgtgtccgg cagaactgac ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag
    36121 atttattatc aatatcagtt acaggattta tcccgtaagg atgactgttg gttgttgacg
    36181 tttgcaggag atcagcaagc gacacacagt gtagtggtac tggcgaacgg gcatcaaatc
    36241 agccgtttca gccaaacatc gtctctgcca gtgtattcgg ttgccggtca ggtcagccat
    36301 attccgacga cacccgaact ggcaaaactg aaacaggtgc tgtgctatga cggttatctc
    36361 acgccgcaaa atccggcgaa tcagcaccat tgtattggtg ccagttatca tcgcggcagc
    36421 gaagagacgg cgtacagtga agacaatcag cagcagaatc gccagcgatt gattgattgt
    36481 ttcccacacg cacaatgggc gaagacggtt gatgtcagta aaaaagaggc gcgctgcggt
    36541 gtgcgttgtg ccacccgcga tcatctgcca atggtaggta atgttcccga ttatgaggca
    36601 acactcgtgg aatatgcgtc gctggcggag cagaaagata aggcggtaag cgcgccggtt
    36661 tttgacgatc tctttatgtt tgctgcttta ggttcgcgcg gtttgtgttc tgccccgctg
    36721 tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga tgccagtacg
    36781 ctggcggcgt taaatccgaa tcggttatgg gtgcggaaat tgttgaaagg taaagcggtt
    36841 aaggccgggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc cttatccgac
    36901 ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc atggtgctcc
    36961 agacgccgca acttactgct gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac
    37021 cagcgcctgg tcacgcggcg acaattcacc agcaccaatg gctttttcca gactctggct
    37081 aacggtcgta tgcaccgcct gagcggagtg gtcatcacca ctttccagtt ctgcgatggc
    37141 taatgtaagg tggccacgca aatacccact ggcaaacaac tcatcatcac tggcatggtc
    37201 aaccataccg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca tcttctttcc
    37261 tcattgtaaa acgtggcgct ggcttcagtt gagcgcttcc ggccacggga actgttccgc
    37321 cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaaacgtg ccgggcgttc
    37381 cggaatgcca tttgttagat aatccatcac ctgcgcatgt actcgccgtt gaaacaccac
    37441 gcggtccggt tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg ggtatcccgc
    37501 cgccacgcag aacaaccagt cgagcgcctg cggcttcact tcaacatctt caaactggct
    37561 ttgcgtttgg gcatcgcgac cgtccgggca ataccagtag ccgaaatcaa cctgctcgcg
    37621 gcgcgctttc ccggcaatac accagtgcga aatctcatga attgcgctgg cataaaagcc
    37681 gtgggcaaag acgattcggt tatacggtac ttccgcatca gcgggaagat agatcggttc
    37741 gtcgtcgcct ttaatcagac gggtattaaa atcatcggca aagcagctat taaaaatttc
    37801 aatcaactgc tcatagtggt gtgtactgtt cattagttca tccccaacca gtggaggatc
    37861 tcctgtccgt ggctatcata aagtaatttg gcactcatca ccgccgagac gataacgatc
    37921 atcgggcgga tcagtttttg tcctttgctc aacaccagtc ttgaacccat gcgcgcgcca
    37981 aggaactgcc ccaccagcat cacaaagccc gtcgcccaaa tcactttgcc gccgagaata
    38041 aacagcagta aaccgccgat gtttgacgtt gcgttgagta atttggcgtg agccgtggct
    38101 ttggcgaggt tgaatccgca cagcgtaaca aaggccagcg cgtaaaacga tccggctgcc
    38161 ggaccaaaga atccgtcata aaaaccgacg cagccaccgg caattaacgc gaagggcaaa
    38221 ccgtacatcc ggcgctggcg atcttcttca cccagctttg gcatcagcaa gaaatagagg
    38281 ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga catcagcctg aacgtactgt
    38341 accaacagtg cgccgctcat tgagccgaca aaggtcatgg caatattaag tttctgatcg
    38401 cttaaactaa ccactttgcg gcgaataaag tagatagtgg cggaaataga gccgccgcag
    38461 gcttgcagtt tattggttgc cagagcatta gcgggagaca tccccgctgc catcaatgcc
    38521 ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc cagcatggcg
    38581 acaaaaaaga gaactcccag caacagcggg gaaaccataa acaggctatt aaacgtttcc
    38641 attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct
    38701 tcttctcagg ctttgttgtt cccggttttg gaggttcaaa ccagctttgc agttctgccc
    38761 cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca ctccagacta tcggcaggac
    38821 aacgtaatcg tacatgcata tgcgcacgat gctggaacca gggtcgcact ttgcgcaacc
    38881 agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa
    38941 tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc
    39001 acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca
    39061 agagctgcgc ggaggtccgg cgtgttttcg gcagttgcag aaagatatcg acatccagtc
    39121 cggtctggtg gctggcgtga ccgccgttga aacgcccacc agcaggcatt cccatatcgc
    39181 caatcaacac cgtacccatg cccagattgc tcacctgact actcaggcgc tggataaaca
    39241 tcaccaggtc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc tgataatgtt
    39301 cggactgtat cggcaacgtg tcagcgccga cgatacaacc attggaaaaa ctgcctatcg
    39361 attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct gccaggctga
    39421 cgctactggc aagcagggcc agcagcgcaa tcgcggtttt gttcattttt taccagcgtg
    39481 gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga tccattaaaa
    39541 cgatcgccag catcgcttct gctatcggca ccgcgcggat cccgacacag ggatcgtgac
    39601 ggcctttggt gatcatctca acttcttcgc caaagcggtt aatggtgcgc cccggcacgg
    39661 taatactgga ggtcggtttc agtgccatat gagcaatgat ttgctgcccg ctgctaattc
    39721 cgccgagaat gccgcccgca tggttactct ggaaaccgtc tttggtgatt tcgtcgcggt
    39781 tctggctgcc acgcagcgcc accacgtcaa aaccatcacc aatttccacg cctttcaccg
    39841 cgttaatgct cattagcgca tgggcgatgt cggcatccag gcgatcaaag accggctcac
    39901 caagcccggc aggaacgcca ctggcaacaa cggtgacttt cgcgccaata gagtcgccct
    39961 cttttttcag cgcgcgcatc agttcatcta acgcgtcgat tttgtccggg tccgggcaga
    40021 agaatggatt ttgctcgacc tgcgaccagt ctttgatttc cagcggaatg tcgcccatct
    40081 gggtcaggca gccgcgaatt tcgatgccaa atttctcagc gagatatttt ttggcaatcg
    40141 cccctgctgc aacgcgcatg gcggtttcac gggcggaaga acgtccaccg ccgcgataat
    40201 cgcgcagacc gtatttttgt tcgtaggtgt aatcggcatg gccaggacgg aaaacgtcct
    40261 taatcgcact ataatcctga gaacgctgat cggtattttc gatcaacaaa ccaatgctgg
    40321 tgccggtagt aacaccttca aaaacaccgg agagaatttt gacctgatct ggctcacgac
    40381 gctgggtggt atagcgcgat gtcccagggc gacgacggtc gaggtcatgt tgcaggtccg
    40441 cttccgtcag cggaatgcct ggcggaacac catcgacgat gcagccgagc gccagcccgt
    40501 gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg
    40561 ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa atgttcacgg
    40621 gccgccagca gctgcgcttt ggtgagcata aacacaccat cgccgccgtt atcaaactcc
    40681 agccaggtga acggaacatc gggatattgt tccataagat gtaccatgct gttgccgact
    40741 tcacaaatca gtacgccatc atcagcaaga taatctgccg cgtttccgag aatgcgacgc
    40801 gtcagtttca ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg gcggtattca
    40861 tttggcaggt cggacatatc ttccgcatcg acatacggcg ggttagtgac aatcaggtcg
    40921 tactgcactt tcggcaaatc acggaacaga tcggaacgaa tcggtatgac gttgtggatc
    40981 agaccgtgtt cttcgatgtt ctgttcggca accgccagcg cgtcgggaga gatatctacc
    41041 gcatcgactt ccgcttccgg gaaggcataa gcacaggcaa tggcgatgca gccgctaccg
    41101 gtacacatat ctaaaatatg ctgcggttgc ttgctgataa gtccggcaaa tttattgttg
    41161 atcaattcac caatcggcga gcgcggcacc agcacgcgtt catcgacgta aaattcatgg
    41221 ccgcagaacc acgctttgtt ggtcaggtaa gctaccggaa tgcgttcgtt gacgcggcgg
    41281 atcacgcgct caacaatacg atgtttttcg ctggaggtca gacgcgcggt gcgcatatct
    41341 tccggaatat ccagcggcag gtagagcgac ggcaatacca gctgtacggc ttcatcccac
    41401 gggttatcgg tgccgtgacc gtaccagata tttgccgcgc tgaagcggct caccgaccag
    41461 cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa aattttatcc
    41521 acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca tgaagatgac
    41581 gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg atcgattgcg
    41641 ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg aaaaagaaaa
    41701 caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg actcgcaaga
    41761 ttaagcagga caccattgtc catcggccac agcgtaaaaa agtcagcgaa gtgcccgtca
    41821 aacggttgat tcaggagcag gttgacgcca gtcactattt ctctgacgaa tttcaaccgt
    41881 tattaaatgc tgatggcccg gtgaagtatg tacgccctgg tgtagaccat tttgaggcga
    41941 aaaagctgcg ccgcggcgat tattcgccgg aattgttttt ggatttacac ggtctgacgc
    42001 agctgcaggc caagcaggaa ctgggcgcgt tgatcgccgc ctgtcgccgc gaacatgtgt
    42061 tttgcgcctg cgtgatgcat ggtcacggga aacatatatt gaagcaacaa acaccgctgt
    42121 ggctggcaca acatccgcat gtcatggcgt ttcaccaggc accaaaagag tatggtggtg
    42181 atgccgcatt gctggtgttg attgaagtgg aagagtggct accgccggag ttgccatgaa
    42241 tattaggggg cggatgcgtt ttgccatttc gccctgaatg ctggtatcga atgccggatg
    42301 cggcgtaaac gcctgatccg gcttatcaaa atgtgcaaat tcaatacgtt gtgattcagt
    42361 tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatc aacgcacaac tacagttcat
    42421 tcagtacata ctggaaacgg catgtagaat caattggctc acggctactg gtattcacct
    42481 gtaccacaat gtcgtgtagc tttctatccg tcaatggttg acgtgagggg gtgactgtca
    42541 cctttgcatt atcacaacca tacgaggtaa aggttaataa gtcagaaaca gcacgacaat
    42601 atgcccctct ggaatcaaaa atactgccat cgcagagttt gccgctttcc atcaggtagg
    42661 tcgaaagcgt agtcgtgatt gtcgaaccat cggattgcaa tcgcagcgct ttattatggc
    42721 taacataatt atcactccag gatatatcaa acgaagcgac gggaattgtc gggcaaatag
    42781 aatcatgaac tgttgggctt gtatacgtac caaatccaaa tgcctcagat actgaataaa
    42841 atgaggcaat ccacggacaa ccataattat cagattctac agtgacgtta aaagtcatat
    42901 atgcacagac aaagacacga gtattgatac cagggcaatt tgaggtgtcc gtacgtctat
    42961 aatcagtcaa aaccagtgtc ggtgccgggc cagatccgct ccaggatatt aacttgaggg
    43021 tgtcgccttt tcttgcattg gagttgtatt gtccgtagct tacttctccc ggcgtcacta
    43081 ccgtgacata gaaatataaa ctgccggaag cagtaacgtt tttcatcgca taataaattt
    43141 tagtactgga aaagcttgca ctcatgagtt tatcaccggc atagcacttt ggtgtttcta
    43201 aactgagagc aagtaatatc agacttgtca aaaatatcgt ccattttttc attttaaaaa
    43261 tactcaaaag ttaccgtcat tgtggcggtg aaatcacccg atgtaacatt tcgtccattt
    43321 acggtctgta accaggcatt aaaattgaca ctgttatttc cctgatttaa tggaaatgag
    43381 gcacctgtgg tactgttaat aggtacagcc gcccctccgg cagtttcaat gccaatccca
    43441 acacccgttg cagaagatga cgaatcaatc gacagaaatc ctggtaagtc ggtatcttct
    43501 gttcccatca aggtcacctt gacattaaac gccgtggtgc ttttgcaatc tttcaactga
    43561 aaaactaacg gtacgcgatc tgactgacca cgttgcatta aatcgctggc agcaattgtg
    43621 gggaaatgaa tttctgcaag taagttgcca ttgattacag gagtacagga tttaccaagc
    43681 aaattaccgg taaaatgcag gttatcggca gctggggccg ttccgctgaa caatatcaga
    43741 ccactgataa gcgcccacgc acggttattc ttcattggta atccacctgt aacgtagccc
    43801 atgcttcaaa gtctgactcg ggtaagtcga caccgctctt ttttacgggt actgccgtta
    43861 gtaccggaag ggatgtttca ttcaccacta agggcgtatg cagcttaaaa tcgctcccgg
    43921 cttgtttgag gtgtattcct aatccattga gatccgtggc tacggccgaa tcgtcaaagt
    43981 cactctgcgt accactccag gtcaacgtca tttccatttg agggtcacgg acagcggtat
    44041 cgcaggtaat ggtatacggc acgacctgtt cgtaattgct accgttaatg tcatcaatat
    44101 tcacgtcccg aaacgtcaac tctatatttt gcccattact aatagagcaa ttgggtggct
    44161 gcactaaata accatgaaaa gagacattcg agagcgccat aaagctaaag ctccacaaca
    44221 ttaagagaga gatccgtttc attgataatc caccacaatt gtggcactgg cgctaaattc
    44281 cgccgccgtt agcgatacac cgctctgcac gacaggaacc gcttccagag ccagtgcgcc
    44341 agatgaaaag ttaaacggta accatgcgcc aggcgttaaa tccaaaatgg agtgatcgct
    44401 tactttctga atacggatac caaaaccggc aatacccgta tcgattaccg tttctccgtt
    44461 aattacggtt gtggttgcct gcatatttaa gcgcaggtcg tccattgcca gattgttgca
    44521 cacaagcgag tagtcaatgg ggcgtttgta acttacccca tcgatcttgg aaatgaagac
    44581 attaccaaac tccacgctcg cacccgttac cgtacagggc ggcggggcat caacgagcac
    44641 acgcaagacc atcgcaaccg atttactccc cgccattaca ggcgtgctca caatgctaca
    44701 cagcaataac cacgcccagg ccgaataagc tcctttcatg ctattgctcc tatccctggc
    44761 ctttgctgtt ttgctcttcg tccacctgac aggtatttcc cgtacagcgg aagaacagcg
    44821 gcaaacgcgc accataatca ttcacatagg tcagcaccgg gacgctatcc attttgactt
    44881 ttaacggcac agaggttttt ggcgatatca ccatggggga aaatcccgct gccggattgc
    44941 catttttctg cgcactggcg ttactgataa ccacgaagta cggcgtcggg ttattgacgg
    45001 taaactcctg accactgagg gttaaggtca ccttatgctg ccacgggttt ttcatgctga
    45061 cgttttccag cgcctttggt cgccagaaca acttaatgcg cgtctgcagc gctatctgta
    45121 gcgtgttagg tttgttcgat ttcggcggga tctcacgcac gttgaaatag aacacgctct
    45181 cgcggtcggc aggcagcttg ttgatatccg gcatgccctg taccttcacc tgaccgttca
    45241 tcatggaatc gatgcgctgc actggcggca ggaccgtcag tggcgagcta atcctgttac
    45301 cctgtccatc ttccatccag ctttgtgcca gatacggcat tttcgggtcg ttattgcgca
    45361 gcgtgacgct gattgattta tcactttcgt taaaaatcag gcgtgtacgg tccggcgtga
    45421 cagaggcctg tactgacagg ctggcggcag ttatcagtaa cgccagcgtc gttgcggccg
    45481 ttctggcaga gcaaaataga ttcgtcatgg atcattcctt aatcacgtta ttaattggct
    45541 gataccgata ctggcgcttc cgttggcatc acctgctggg tctgctcctg gatcaccggc
    45601 ttaatttcat gaggcatcga aggagatatc gcccctgttt gctggcatgg taataacagg
    45661 ccattgaaca gatcggctgg cagcgggtca ggcagatgaa tatcgcagtg ggccacaccg
    45721 ccccatgaaa cgatcatatg ctcgccaggt tttacgcccg cgaggtagac gttgccgtca
    45781 tcgtcaacca gaccgacgtt ctgcgcgctg tcgtttttca cttccgcgcc aaacggcgga
    45841 taactgccat cttgcagacg cagcaccgcc atcgcttttt gcccgctgat caccgagaac
    45901 ttacggtagc caatggcccc ttcggtaagc gtggcctgca ccacggactg ggtggcttcg
    45961 gcgttttccg gcaggttgtt taggtcgata tacgcctggt tgcggtagta gttgttgacg
    46021 tctgccacca ctgccttacc gaacatattg gtgtaaaccg ccgcgccatt tccttccacc
    46081 ggaacaccag ccacaccgtc ggcatcaatc agcagacgcg taccgcccat attctgggta
    46141 cggtggagcg cgccaccttg tgcggtgagc gtcgcgccgc cctgtaagga aatacccgcc
    46201 gaggtgtact gcccttcatg gtagttagcg ctgagatcga cctgcgccag cgatccgtcg
    46261 tggctgtaat aaccgtcaac gctggagtga ttgtcgctgg tgcctacgtt caactggtaa
    46321 tgggttgcgt catcgacacg gctgaaatac cccacctggc tgctgtccga accgctgccg
    46381 tagttgccgt tatagctgat ggtgctgctg tcaccccacg gcatactgag cgatatgtac
    46441 acacctttat cggcctggtt gtcatactcg tagcggtatc cggtcatgga aatgctcatg
    46501 ttgcggatac tgcccaggtt gaagtagtgc gagagcatga cgttgtagtt ggtctgttcg
    46561 tcgcgatccc agtaggtatg gcgggtgtag ttgagataaa cagacacacc ggcatccctg
    46621 aagttctggt tataggtggc ggtgtacatc tctttgtcgt tgccggtgcg caccatttcg
    46681 ctgtcgctgg catcgagata ctcgctcatg gtcatgaagt tctcttccga gaagcggtat
    46741 ccggcaaaag tgacgcggct gttcagttca tcgaagtctt tggaatagct caggcgaaac
    46801 gagttgccgt ccagtgaacc tttcccgtag gcggtctctt tatccagacg ggtatgcgag
    46861 tgggtgatat caaaggccac cgcaccaaac acagacaggt cgcgaccgac gccaagcgcc
    46921 gccgactgat agtgttcatc tgccagcgcc ccgccgtata gcgaccagcc gttggcaatc
    46981 ccccaggaag cttcgccgcc ggagaagaaa ccgccttcca cgtggtgccc ccactcctgc
    47041 gggcggccca tcatcagttt atagcgcacc tggccggggc gagtcaggaa cggcatcgag
    47101 gcggtgttga tgtcatattc ctgcacctga ccgttctgtt cttcaatgcg gatatgcagc
    47161 gtaccggaga ccgaatcgcc aagatcctgg atgcggaacg gccccgccgg gacctgggtt
    47221 tcgtagatga cgcggcccaa ctggctgacg gtcactttcg cggtggtgtg cgccacgccg
    47281 gagatatccg gcgcatagcc gcgcagattc ggcggcaaca tttgatcgtc ggtgctgata
    47341 ctgccaccca cgtagttaaa gccgtcgaaa atatcagaat tcaggtagtc ttcgccaagg
    47401 ccaagtttgg cttttagcga cggtaaggct cgccaggcgt agtagcggct ccactcccag
    47461 tttttttgcg tgtcatcacc gttgataaca tcgtcatcat tgcttttact atgcaaataa
    47521 tcagtctgcc agtcggcacg aagacgccat gcgccgaggt tcaccccaac cgtcccgtta
    47581 ccgctaattt cattgctgtc atccccgcca ttttcttcat gtcgtgtctg ggcggtaata
    47641 ctgtagtcag caattaaacc agatataccg tcatcccagc gtgaaggcgg atcccagttg
    47701 atgtcggtat attcaaggta agcctggggt aatgaaatga ctaacgctga ctgactcagg
    47761 tcagctttaa tatcaatgcc ttccagttga ccgggtttca ggcatttgcc gtcgtggatc
    47821 cattgcaggt tttttgccac atcctctttt aagccaaact gcgcgaccag ttcaggcgtc
    47881 aggcaggcat aggttttact ggcatcgttc tcagaggcgt accagtaaat atcgtattct
    47941 tccgtcagcg gctgtttatt tagttgaacc tgtaaattgt atttcccagg ttcgacataa
    48001 ccctggctgg aaaatcgctt cagatcgatt tttgtgtcgc cttttaactc cagaaaacgg
    48061 gaatcaaact gaatttcatt ttcagcccag gcattgacat aactcccgga cattgccaat
    48121 gcaacgcagc agggaagtac ccgcagtcgg aaaagagaat ggtcaggcat acacgtatcc
    48181 atccgtaatt attataattt gcgcgaattc catttaaaaa ccccatagcc cgaaagctat
    48241 gggggtatca cttagagata aataatctgg aaggtcgtgt tggcttcaaa attacctaaa
    48301 tctggcgcat cagcagcgcc caccagccag gctttaaagt ccagcgtctg cttcgctttg
    48361 cctttgttgg tcgccgtatc gtttacgatt ttctgttcga tacccgcgcc gcttttataa
    48421 gaggttccct gagcgtcacc aatggccagg ctgacattgt taaatgccgc accagtatcg
    48481 gtattgtaaa tggtgtagta attgccgctg ttggtagaag acgcgttacc ggtgaaggta
    48541 gtggtcatcg ttgtctgggt gtcaaacaca cagtcttgca gatggatctg gaaagctttc
    48601 ggtgtagaag tcccgttatt tttcaggatc cctgaaccaa tgtcacccat atcaacttcc
    48661 agtttgtgat catcaggaac gatagagcac ggggaatctt caatggtgcc gtagaaacgt
    48721 gctgtaccat tgttgccagc ggcgattgtt gaagtagaag cgaacgcacc cattaccatt
    48781 gttgcagcaa tagctgtttt tacaaactta ctcattattt gtccttaaac cataaataat
    48841 taaaaatata aacattgcaa tacattgatt cagtcaatag caattattta tacccagcca
    48901 ttgaataagc tatccgatct aactaacaag aaaataggtc gcataagaag atattctttg
    48961 gtgtgggcgg atggtgatat tttgtcttta ttgtttcaaa ttatatttac ttaa
//
LOCUS       DAZGXS010000020        48172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGXS010000020 DAZGXS010000000
VERSION     DAZGXS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            286..1602
                     /gene="fucP"
                     /locus_tag="ACHKG5_001300"
     CDS             286..1602
                     /gene="fucP"
                     /locus_tag="ACHKG5_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0867439.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1635..3410
                     /gene="fucI"
                     /locus_tag="ACHKG5_001301"
     CDS             1635..3410
                     /gene="fucI"
                     /locus_tag="ACHKG5_001301"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0867440.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3519..4937
                     /gene="fucK"
                     /locus_tag="ACHKG5_001302"
     CDS             3519..4937
                     /gene="fucK"
                     /locus_tag="ACHKG5_001302"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0867441.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4939..5361
                     /gene="fucU"
                     /locus_tag="ACHKG5_001303"
     CDS             4939..5361
                     /gene="fucU"
                     /locus_tag="ACHKG5_001303"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0867442.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5419..6150
                     /gene="fucR"
                     /locus_tag="ACHKG5_001304"
     CDS             5419..6150
                     /gene="fucR"
                     /locus_tag="ACHKG5_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0867443.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACHKG5_001305"
     CDS             complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACHKG5_001305"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0867444.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACHKG5_001306"
     CDS             complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACHKG5_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0867445.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACHKG5_001307"
     CDS             complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACHKG5_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0867446.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACHKG5_001308"
     CDS             complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACHKG5_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0867447.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9388..10593
                     /gene="csdA"
                     /locus_tag="ACHKG5_001309"
     CDS             9388..10593
                     /gene="csdA"
                     /locus_tag="ACHKG5_001309"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0867448.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10593..11036
                     /gene="csdE"
                     /locus_tag="ACHKG5_001310"
     CDS             10593..11036
                     /gene="csdE"
                     /locus_tag="ACHKG5_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0867449.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACHKG5_001311"
     CDS             complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACHKG5_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0867450.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACHKG5_001312"
     CDS             complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACHKG5_001312"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0867451.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13438..13514
                     /locus_tag="ACHKG5_001313"
     tRNA            13438..13514
                     /locus_tag="ACHKG5_001313"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13472..13474,aa:Met,seq:cat)
     gene            complement(13588..14841)
                     /gene="amiC"
                     /locus_tag="ACHKG5_001314"
     CDS             complement(13588..14841)
                     /gene="amiC"
                     /locus_tag="ACHKG5_001314"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0867452.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15073..16404
                     /gene="argA"
                     /locus_tag="ACHKG5_001315"
     CDS             15073..16404
                     /gene="argA"
                     /locus_tag="ACHKG5_001315"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0867453.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16466..18292)
                     /gene="recD"
                     /locus_tag="ACHKG5_001316"
     CDS             complement(16466..18292)
                     /gene="recD"
                     /locus_tag="ACHKG5_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0867454.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18292..21834)
                     /gene="recB"
                     /locus_tag="ACHKG5_001317"
     CDS             complement(18292..21834)
                     /gene="recB"
                     /locus_tag="ACHKG5_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0867455.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(21827..24715)
                     /gene="ptrA"
                     /locus_tag="ACHKG5_001318"
     CDS             complement(21827..24715)
                     /gene="ptrA"
                     /locus_tag="ACHKG5_001318"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0867456.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24891..28259)
                     /gene="recC"
                     /locus_tag="ACHKG5_001319"
     CDS             complement(24891..28259)
                     /gene="recC"
                     /locus_tag="ACHKG5_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0867457.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28272..28595)
                     /gene="ppdC"
                     /locus_tag="ACHKG5_001320"
     CDS             complement(28272..28595)
                     /gene="ppdC"
                     /locus_tag="ACHKG5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0867458.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28580..28987)
                     /gene="ygdB"
                     /locus_tag="ACHKG5_001321"
     CDS             complement(28580..28987)
                     /gene="ygdB"
                     /locus_tag="ACHKG5_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0867459.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(28984..29547)
                     /gene="ppdB"
                     /locus_tag="ACHKG5_001322"
     CDS             complement(28984..29547)
                     /gene="ppdB"
                     /locus_tag="ACHKG5_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0867460.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29538..30008)
                     /gene="ppdA"
                     /locus_tag="ACHKG5_001323"
     CDS             complement(29538..30008)
                     /gene="ppdA"
                     /locus_tag="ACHKG5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0867461.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30192..30986)
                     /gene="thyA"
                     /locus_tag="ACHKG5_001324"
     CDS             complement(30192..30986)
                     /gene="thyA"
                     /locus_tag="ACHKG5_001324"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0867462.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30993..31868)
                     /gene="lgt"
                     /locus_tag="ACHKG5_001325"
     CDS             complement(30993..31868)
                     /gene="lgt"
                     /locus_tag="ACHKG5_001325"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0867463.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32019..34265)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_001326"
     CDS             complement(32019..34265)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_001326"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0867464.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34278..34808)
                     /gene="rppH"
                     /locus_tag="ACHKG5_001327"
     CDS             complement(34278..34808)
                     /gene="rppH"
                     /locus_tag="ACHKG5_001327"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0867465.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35161..35307)
                     /gene="ygdT"
                     /locus_tag="ACHKG5_001328"
     CDS             complement(35161..35307)
                     /gene="ygdT"
                     /locus_tag="ACHKG5_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0867466.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35493..36182
                     /gene="mutH"
                     /locus_tag="ACHKG5_001329"
     CDS             35493..36182
                     /gene="mutH"
                     /locus_tag="ACHKG5_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0867467.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36251..36964
                     /gene="ygdQ"
                     /locus_tag="ACHKG5_001330"
     CDS             36251..36964
                     /gene="ygdQ"
                     /locus_tag="ACHKG5_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0867468.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37102..37320
                     /gene="ygdR"
                     /locus_tag="ACHKG5_001331"
     CDS             37102..37320
                     /gene="ygdR"
                     /locus_tag="ACHKG5_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0867469.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37428..38468
                     /gene="tas"
                     /locus_tag="ACHKG5_001332"
     CDS             37428..38468
                     /gene="tas"
                     /locus_tag="ACHKG5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0867470.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38500..39693)
                     /gene="lplT"
                     /locus_tag="ACHKG5_001333"
     CDS             complement(38500..39693)
                     /gene="lplT"
                     /locus_tag="ACHKG5_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0867471.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39686..41845)
                     /gene="aas"
                     /locus_tag="ACHKG5_001334"
     CDS             complement(39686..41845)
                     /gene="aas"
                     /locus_tag="ACHKG5_001334"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0867472.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            42431..43462
                     /gene="galR"
                     /locus_tag="ACHKG5_001335"
     CDS             42431..43462
                     /gene="galR"
                     /locus_tag="ACHKG5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0867473.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43469..44731)
                     /gene="lysA"
                     /locus_tag="ACHKG5_001336"
     CDS             complement(43469..44731)
                     /gene="lysA"
                     /locus_tag="ACHKG5_001336"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0867474.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44853..45788
                     /gene="lysR"
                     /locus_tag="ACHKG5_001337"
     CDS             44853..45788
                     /gene="lysR"
                     /locus_tag="ACHKG5_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0867475.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45775..46467)
                     /gene="ygeA"
                     /locus_tag="ACHKG5_001338"
     CDS             complement(45775..46467)
                     /gene="ygeA"
                     /locus_tag="ACHKG5_001338"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0867476.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46596..48014)
                     /gene="araE"
                     /locus_tag="ACHKG5_001339"
     CDS             complement(46596..48014)
                     /gene="araE"
                     /locus_tag="ACHKG5_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0867477.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11835 a        13077 c        11907 g        11353 t
ORIGIN      
        1 gcataaacaa ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg
       61 gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa
      121 gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt
      181 tcagacaggc aaatattcac tgatatgaag cccgaactcg ctagttttgc acttttgaaa
      241 acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca
      301 atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt
      361 attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac
      421 gacattttat tacctcaatt ccagcaggct tttacactga caaatttcca ggctggcctg
      481 atccaatcgg ccttttactt tggttatttc attatcccaa ttcctgctgg gatattgatg
      541 aaaaaactca gttataaagc aggaattatt accggattat ttttatatgc cttgggtgct
      601 gcattattct ggcccgccgc agaaataatg aactacacct tatttttagt tggcctattt
      661 attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt tacggtatta
      721 gggccggaaa gcagtggtca cttccgctta aatcttgcgc aaacatttaa ctcatttggc
      781 gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg
      841 caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta
      901 ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg
      961 atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga
     1021 tcgttctccg catcgctttc tcgcctggcg cgtattcgcc actggcgctg ggcagtatta
     1081 gcgcaattct gctacgttgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct
     1141 gtagaagaaa ttccaggcat gactgcaggc tttgccgcta actatttaac cggaaccatg
     1201 gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac
     1261 aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct
     1321 ggcagtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac
     1381 ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc
     1441 ttcatcgtta tgaccatcat tggcggcggt attgtcactc cggtcatggg ttttgtcagt
     1501 gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc
     1561 tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt tccgaataaa
     1621 gtgaggaatc tgtaatgaac aaaatcagct taccgaaaat tggtatccgc ccggttattg
     1681 acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag
     1741 ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg
     1801 tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa gaaaaattca
     1861 gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat ggcagtgaaa
     1921 ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc actgaacgtc
     1981 ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc atcccggcat
     2041 tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg
     2101 aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa
     2161 gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt gatcacaact
     2221 tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc
     2281 gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc tgggctgata
     2341 aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat gccgagcaaa
     2401 gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag
     2461 gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg
     2521 ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt gacaccgccg
     2581 aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga
     2641 ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg
     2701 ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg
     2761 ggcataaact ggatggactg gcagaacacg gcatcatcca tttgatcaac tccggttctg
     2821 ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc
     2881 actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg
     2941 cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg
     3001 tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg
     3061 cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca
     3121 actcaacctg gccaaccacc tggtttgcac cgcgcctcac cggtaaaggg ccgtttacgg
     3181 atgtgtactc ggtaatggcg aactggggcg ctaaccatgg ggttctgacc atcggccacg
     3241 ttggcgcaga ctttatcact ctcgcctcca tgctgcgtat cccggtatgt atgcacaacg
     3301 ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag
     3361 gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa tacattactc
     3421 cctgatgtga tgcccggtcg ctccggctgc cgggcctgaa caagcaagag tggttagccg
     3481 gataagcaat gttatccggc tatattgcag gagcgatcat gaaacaagaa gtgatcctgg
     3541 tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg
     3601 ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc
     3661 agtggtcttt agacgccatt ttgcaacgct ttgctgattg ctgtcggcaa atcaatagtg
     3721 aactgactga atgccacatc cgcggtatcg ccgtcaccac ctttggtgtg gatggcgctc
     3781 tggtagataa gcaaggcaat ctgctctatc cgatcattag ctggaaatgt ccgcgaacag
     3841 cagcggttat ggacaatatt gaacggttaa tctccgcaca gcggttgcag gctatttctg
     3901 gcgtcggagc ctttagtttc aatacgttat ataagttggt gtggttgaaa gaaaatcatc
     3961 cacaactgct ggaacgcgcg cacgcctggc tctttatttc gtcgctgatt aaccaccgtt
     4021 taaccggcga attcactact gatatcacga tggccggaac cagccagatg ctggatatcc
     4081 agcaacgcga tttcagtccg caaattttac aagccaccgg tattccacgc cgactcttcc
     4141 ctcgtctggt ggaagcgggt gaacagattg gtacgctaca gaacagcgcc gcagcaatgc
     4201 tcggcttacc cgttggcata ccggtgattt ccgcaggtca cgatacccag ttcgcccttt
     4261 ttggcgctgg tgctgaacaa aatgaacccg tgctctcttc cggtacatgg gaaattttaa
     4321 tggttcgcag cgcccaggtt gatacttcgc tgttaagtca gtacgccggt tccacctgcg
     4381 aactggatag ccaggcaggg ttgtataacc caggtatgca atggctggca tccggtgtgc
     4441 tggaatgggt gagaaaactg ttctggacgg ctgaaacacc ctggcaaatg ttgattgaag
     4501 aggctcgtct gatcgcgcct ggcgcggatg gcgtgaaaat gcagtgtgat ttattgtcgt
     4561 gtcagaacgc tggctggcaa ggagtgacgc ttaataccac gcgggggcat ttctatcgcg
     4621 cggcgctgga agggttaact gcgcaattac agcgcaatct acagatgctg gaaaaaatcg
     4681 ggcactttaa ggcctctgaa ttattgttag tcggtggagg aagtcgcaac acattgtgga
     4741 atcagattaa agccaatatg cttgatattc cgttaaaagt tctcgacgac gccgaaacga
     4801 ccgtcgcagg agctgcgctg ttcggttggt atggcgtagg ggaatttaac agcccggaag
     4861 aagcccgtgc acagattcat tatcagtacc gttatttcta cccgcaaact gaacctgaat
     4921 ttatagagga agtgtgaaat gctgaaaaca atttcgccgt taatttctcc cgaactattg
     4981 aaagtgctgg cagagatggg acatggagat gaaattattt tttccgatgc tcactttccc
     5041 gcccattcga tgggaccgca ggtgatccgc gctgatggcc tgttggtgag cgacttgctc
     5101 caggcgatta tcccgttatt tgagctggac agttatgcac cgccgctggt gatgatggca
     5161 gcggtagaag gtgacactct cgatcctgaa gtagaacgac gttaccgtaa tgcgctttca
     5221 ctacaagccc cgtgtcctga catcatccgc atcaatcgtt ttgcgtttta tgaacgggcg
     5281 caaaaagcct ttgcgatcgt tatcacaggc gaacgagcga agtacgggaa tattctttta
     5341 aaaaaagggg taacaccgta atctcatacc ggtacgcccg catgacgcgg gcggttatcg
     5401 aatgatgggg tgaaaaatat gaaagcggca cgccagcaag cgatagtcga cctgctgctg
     5461 aaccatacca gcctgaccac ggaagctctc tctgaacagc taaaggtcag taaagaaacc
     5521 attcgtcgcg atctcaatga attacagacg cagggtaaaa ttctgcgcaa tcatggacgc
     5581 gctaaatata tccaccgtca aaatcaagac agtggcgatc cctttcacat caggctgaaa
     5641 agccattatg cgcataaagc agatatcgcg cgcgaggcgc tcgcgtggat tgaagaaggg
     5701 atggtgatag ccttagacgc cagttcaact tgctggtatc tggcacgcca gttgcctgac
     5761 atcaacattc aggtcttcac caatagccat ccgatttgcc atgaactcgg taaacgcgaa
     5821 cgcattcaac tgatcagttc cggcggcaca cttgagcgca aatatggctg ttacgtcaat
     5881 ccctcgctga tttcccaact taaatcgctg gaaatcgatc tgtttatttt ttcttgtgaa
     5941 gggatcgata gcagcggcgc actgtgggac tccaatgcta tcaacgctga ttacaaatca
     6001 atgctattaa aacgcgccgc gcaatcgttg ttattgattg ataaaagtaa atttaatcgt
     6061 tcaggggaag cccgcatcgg gcatctggat gaggtaacgc acattatttc tgatgaacgc
     6121 caggttgcaa cttctttggt aacaggctga cggaaagggt agcaggccgg agacgacgcc
     6181 ccggccttgc ctgttatcgc tcgtcgcgac gaccacccac cgcagcccag atacggcgga
     6241 cgtgcaccgt cacttcttcg cgatcgtgat acaactgccg cgcctgaatc tgagcattta
     6301 tgccatgttc atcaagctgt gcctgaatat acgccagatt gtgtgacact tcttcgtagc
     6361 gttttttcat cggcagtttg aggttgaaaa tggtttcacg acaccagcca ttaaccagcc
     6421 actgcgccat caatgccgca actttcgccg gtttttcaac catatcgcat accatccagg
     6481 agatattgct gcgcgtcgga cggaatttga aaccgtcttc ccgcagccac gtcacctgtc
     6541 cggtatccat cagactttgc gccatcgggc cgttgtcgac ggaataaacc cacatgttgc
     6601 gcttcaccag ttggtaggtc cagccgccag ggcaagcgcc taaatccacc gcccacatcc
     6661 cgttcgccag gcgttcatcc cactcatccg caggaataaa cacatgaaat gcctcttcca
     6721 gtttgagcgt ggaacgactc ggcgcatctg ccggaaattt caggcgcgga atgcccatat
     6781 agaacggcga attattgttg ctgtatgagt aaccggtata gcagcagcct ggtgcaataa
     6841 agaatacatg cacaaccgga cgcttcggcg tttcatagtt cgccagcacc ccggcatcgc
     6901 gcaaggcagc gcgtagcggc acggtgaatt tacggcagaa tttcagcaac tctttgcttt
     6961 cgttggtatc ggcaacttca acacgcagtt caccgccctt ctctactacg ccctgcaaca
     7021 tgccgacaat gggggtaata cgatcttctg gcggcaaatg ctgcaagagt tcccccacca
     7081 caaaccactg gcgggcaaaa attaatgaac tgaacggcag ctcacggatt aacttatcgc
     7141 catcatcagg ttgataacat tcataaatga cataacccgc attctctttc acgcgggcaa
     7201 aaccgaaaat ctcccgctgg ccggctttat cggtaatttc tgcggcgcac tctttttcaa
     7261 agcccggacg gcacagcaat acaaccttat tcatgactta cgcccttgcg ctttaaacgg
     7321 atagcaccaa ctaacattaa cgcccagccc gcaaggaagc tcacgccgcc aaccggagtg
     7381 acaaacgccc acaaacgcag atgggacagt gccaggcaat aaaggctgcc gctgaacagc
     7441 accgtgccta acgcgaggaa aacgctactc cagtaaaacc agatactgat gcgacgctgc
     7501 attgccaccg ccagacctaa gatcgccagc gtatgaaacg cctggtattc gaggccggtc
     7561 tggatccagc ccatctcaac ggcccccatg gttttactta acacatgcgc gccaaaagcg
     7621 cccagtgcca caaaaatgaa gccgctaatg gcggcgaaaa tcagcataaa acggctggtc
     7681 atggtcgtac cctacgtaaa ttattgttca taacgaaagc ggaatttttc ttgttcagca
     7741 gcggctttcg ccaggatcca ttggcgaaag gcggctattt tacccagttc tgcctggctg
     7801 tcatgacaaa ccagataaaa agcgttttta ctgaccagaa catcattaaa cgggcaaaca
     7861 agacgtccgg cttcaatttc agattgcgcc atcacgttat ttgccagcgc cactccctgc
     7921 ccgtgaatag ccgcttgcag caccatggcg ctatggctaa aaattggccc ttgctgaacg
     7981 ttgatatgat ttaaccccaa ttgacgagta tatgtctgcc agtcacggcg tgacgcatca
     8041 tgtaataacg tatgtttagc cagatcttcc ggggtcttca agggtttttc gccagtcagc
     8101 agtagcggcg aacacaccgg caataaatat tcggcgtaca gtttttccac ccgtagcccc
     8161 ggccagttgc cccgaccata aaatatcgcc acatcaacat catccgccag cttatcttcc
     8221 tgacgatcaa ccgcctggat tcgaacgtca attcccggat aagctgaatt aaagctggaa
     8281 agtcgcggaa ccaaccaatg aatggcgaaa ctggggagta aactgaccgt caacgccccc
     8341 ttggcgctac gggcctggag tttacgcgtc gcttcggtta attgcgaaaa tatctctttg
     8401 atatcgagga aatagctttg cccttcctcg gtcagcagga gtgaacgatt acggcggcgg
     8461 aacagtttta gccccaaaaa atcctcaaga gacttgattt gatgacttac tgcggcctgg
     8521 gtcacaaaaa gctcttctgc tgcgcgagtg aaacttaaat ggcgtgctgc ggcatcaaaa
     8581 actcgtaagg catttagcgg tggtaatcgt ttagacatgg cttttaaact ttgatgttaa
     8641 atgaatttaa caattagatc acactgtgta acctattagt ttttttaatc tgagccatta
     8701 taaattgtcc gttgagcttc taccagcaaa tacctatagt ggcggcactt cctgagccgg
     8761 aacgaaaagt tttatcggaa tgcgtgttct ggtgaacttt tggcttacgg ttgtgatgtt
     8821 gtgttgttgt gtttgcaatt ggtctgcgat tcagaccatg gtagcaaagc tacctttttt
     8881 cacttcctgt acatttaccc tgtctgtcca tagtgattaa tgtagcaccg cctaattgcg
     8941 gtgctttttt ttaccttgcg atcgcgaatt actgatccag ttcgaccatc tctttcacgt
     9001 cggtacggtt gatctgctgt ttgttgccat tagcgtcttt atacgaaatc ataccggtat
     9061 cgttatcagt ctgtggtttg ccgtcagaga cgatggtacg tccgtcattg gtgtgcatca
     9121 catagttcga accggaacag gcgctcaggg caaaagtcag catacaggca gaaataattg
     9181 cggcagtctt tttcatcatc ttctccttaa agcgattaat aaatcttaat agtattgatt
     9241 aacaggctaa aattaacgcc taacactatt cagcatatgt tactgacgcg gcttcgccag
     9301 gatatccaga taattctgat ggttagcact ctccttgtat caaagtgaat tttgcgtcac
     9361 gatcggtgca tcaagccgag gagtaccatg aacgttttta atcccgcgca gtttcgcgcc
     9421 cagtttcccg ctctacagga tgcgggcgtc tatctcgaca gcgccgcgac cgcgcttaaa
     9481 cctgaagccg tggttgaagc cacccgacag ttttacagtc tgagcgccgg aaacgtccat
     9541 cgcagccagt ttgccgaagc ccaacgcctg accgcgcgtt atgaagctgc acgagagaaa
     9601 gtggcgcaat tactgaatgc accggatgat aaaactatcg tctggacgcg cggcaccact
     9661 gaatccatca acatggtggc acaatgctat gcgcgtccgc gtctgcaacc gggcgatgag
     9721 attattgtca gcgtggcaga acaccacgcc aacctcgtcc cctggctgat ggtcgcccaa
     9781 caaactggag ccaaagtggt caaattgccg cttaatgcgc agcgactgcc agatgtcgat
     9841 ttgctgccgg aactgattac tccccgtagt cggattctgg cgttgggtca gatgtcgaac
     9901 gtaactggcg gttgcccgga tctggcgcga gcgattacct ttgctcattc agccgggata
     9961 gtggtgatag ttgatggtgc tcagggggcg gtgcatttcc ccgcggatgt tcagcaactg
    10021 gatatcgatt tctatgcttt ctctggtcac aaactgtatg gcccgacggg tatcggcgtg
    10081 ctgtatggca aatcagaact gctggaagcg atgtcgccct ggctgggcgg cggcaaaatg
    10141 gttcacgaag tgagttttga cggcttcacg actcaatctg cgccgtggaa actggaagca
    10201 ggaacgccaa atgtggctgg tgtcatagga ttaagcgcgg cgctggaatg gctggcagat
    10261 tacgatatca accaggccga aaactggagc cgtagcttag caacgctggc ggaagatgcg
    10321 ctggcgaaac gtccaggctt tcgttcattc cgctgccagg attccagcct gctggccttt
    10381 gattttgctg gcgttcatca cagcgatatg gtgacgctgc tggcggagta cggtattgcc
    10441 ttgcgggccg ggcaacattg cgctcagccg ctactggcag aattaggcgt gaccggcaca
    10501 ctgcgcgcct cttttgcgcc atataataca aagagtgatg tggatgcgct ggtgaatgcc
    10561 gttgaccgcg cgctggaatt attggtggat taatgacgaa cccgcaattc gccggacatc
    10621 cgttcggcac aaccgtaacc gcagaaacgt tacgcaatac cttcgcaccg ttgacgcaat
    10681 gggaagataa atatcgccag ttgatcatgc tggggaaaca gcttccggct ttgccagacg
    10741 agttaaaagc gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg ctgggatata
    10801 ccgtggctga aaacggtaaa atgcacttct ttggcgacag cgaaggacgt atagtgcgcg
    10861 gcctcctggc ggtgctgttg actgccgttg aggggaaaac cgccgccgaa ttgcaggcac
    10921 agtcgccgct ggcattgttt gatgagctgg gattgcgtgc gcaacttagc gcctcacgca
    10981 gccaggggtt aaatgcgtta agcgaggcga ttatcgctgc gacgaagcag gtttaagcac
    11041 agaaccgggt cggataagac gtttgcgtcg catccgacct gattgtttac ccctgacgcg
    11101 ccgctttcgc catcatcttc ttcagcgcat gagaaaccgc aacaaaacca aaggtggcgg
    11161 tcaccatcgt tgccgcgcca aatccagatg cacaatccat ccgctttggt ccttccgccg
    11221 tggctttcat cgcacacacc gtaccgtctg actgcggata caccaatgct tcggtagaga
    11281 acacgcaatc cacaccgagc ttacctttac tgtttttcac tacgccaaaa tcgcttttca
    11341 ggcgctcacg caacttcgcc gccagcgggt cctgaatcgt tttcgccaga tcggtaacct
    11401 gaatctgcgt tgggtcaatc tgcccacccg cgccaccggt cgtgaccagc gggattttat
    11461 tgcgccgaca ataagcaatc agcgccgctt tgggccgtac actatcaatg gcatcaatca
    11521 cgtacgaata acccacgctc atatgctgcg ctacgttatc cggcgtcacg aaatcatcca
    11581 ccaccgttac acagcactcc gggttaatct ggcgaatacg ttccgccatg acttccgctt
    11641 ttgccagccc aacgttatcg cgcagggcat gaatttgccg attggtattg gtgacgcaca
    11701 catcatccat atcgataagc gtgattgcgc caatccccgt gcgcgccagc gcttccgccg
    11761 cccaggaacc gacaccgcca ataccgacca cacaaatatg cgcgtcagca aacagttgca
    11821 acgctttttc accatacaga cgcgctgtgc caccaaaacg ctgacgccat gcatcactaa
    11881 ttaccacaga catagcacct cagaattaaa aagggcgagg ttatccctca ccctaaacgc
    11941 ataaatgcct gatgcgctac gcttatcagg cctacataac tcctgcaata ttttggattt
    12001 gcatgatttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt
    12061 agtcaataat caggatgtga ggttacccct caccctgtca tatccgtaaa aacggcatac
    12121 agaatatcac atcagccgct aaagacgtta cctgcgcccg gggcggtttt cagcacccag
    12181 acacgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
    12241 taaatatcga agtgttggcc tttgattgcg ccaccgacat ccaacgccac catcagacgc
    12301 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag
    12361 gttgtacctg gtgggataat ggaacgatca gaggcaacag aagcacggcc aaccagcggc
    12421 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaaaac gaaagacggg
    12481 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
    12541 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
    12601 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
    12661 ccaaagtcga tatacccact accctgcaca tccataatga aattatccat cagagagttg
    12721 ctgtaagcga ggatgtactt atcgctcaat gcacccgcgt agatctccgc acgagacggc
    12781 agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc ctggcgggta
    12841 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
    12901 gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt atcaccgcct
    12961 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
    13021 gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca
    13081 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc
    13141 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
    13201 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
    13261 caacgaagat aacaaaccac cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
    13321 ttgcacaaaa aataggcttt agtgatttgt ttttgttcaa aatcatgcca gatccgtgat
    13381 cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc
    13441 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    13501 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct ggtctcctat
    13561 aaaaaagcgc cattcagcgc ctttttatca tccccttctc gccagggtcg ccccatcggc
    13621 aaaatacgct ttaattcccg caagaataga ctccgcaact tcctgctgga aggtcgccgt
    13681 tttcagttta cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg
    13741 aatatctggt gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt
    13801 gttgatttta ccgagcttat tcagcaccgc tttaccaaac ttaaggctgt cagcaatggt
    13861 cagcgactgc accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac
    13921 gccaccaatc aagtcggagg cgttctgggt ttgtgccaga tattttgccg cagtactggt
    13981 tgcacctttg gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc
    14041 gtcggcatgg atagagacaa acaggtcagc acgctgtttc tgagcttttg ctacgcgcac
    14101 ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt
    14161 ctcgatcaga gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt
    14221 gtatttcccc accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat
    14281 cggacgatcg cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt
    14341 ttcgaggtcg cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg
    14401 tgcattggcc ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag
    14461 ggcaaacagc tgcggtttta cgttttgctt taattcaaaa accatccgta cggtttgcgg
    14521 gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc
    14581 catccccttg agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttccgg
    14641 gttactcaac gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt
    14701 gtagctggac gcaggccaga cgcgcaccgc cacgacctgg ctgaccgcag ccagactgac
    14761 ctgacttacg ctcaatagcc acatggcacc cgcgccttgc agtaaacgac gacggctgat
    14821 tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag
    14881 taaacgacta atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac
    14941 ggtaaacatt ctttttatat tcacgccatt actgataaaa aagtcgctct cgcataaaat
    15001 ttacacttgc accctgcgaa aaaacagaat aaaaatacaa taaattcgaa taataatgca
    15061 aagaggtgtg ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg
    15121 gttccctata tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc
    15181 attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc
    15241 atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat
    15301 caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg
    15361 gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc
    15421 aataacacgc cgctacaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc
    15481 cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt
    15541 gatgaagacg cgatccatcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc
    15601 gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg
    15661 gccatcaaac tgaaagctga gaagatgatt ggtttttgct cttcccaggg cgtcactaat
    15721 gacgacggtg atattgtctc cgaacttttc cctaacgaag cgcaagcgcg ggtagaagcc
    15781 caggaagaga aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa
    15841 gcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg
    15901 ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag
    15961 cagattcgtc gcgcaacaat caacgatatc ggcggtattc tggagttgat tcgcccgctg
    16021 gagcagcaag gtattctggt acgccgttct cgcgagcagc tggagatgga aatcgacaaa
    16081 ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta tccgttcccg
    16141 gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagctcatca
    16201 cggggcgagg ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa
    16261 ttgtttgtgc tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg
    16321 gatattgatt tattgcccga aagcaaaaag cagttgtaca actaccagcg taaatccaaa
    16381 gtgttgatgg cggatttagg gtaagcgatg aaaatcgtcg gatgcgacat gcgtaacact
    16441 cgtacgtcgc atccggcaat tacgtttatt cccgtgaact aaacaacgcc gccagaccac
    16501 tgcgccgctc agtacgagtg gcgattgccg cacttaatat gcgctcatcg gcatacagcg
    16561 acagacggcg acgcgcgcgg gtcaccgcgg tataaaccag ctctcgcgtt actaccggcg
    16621 tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta
    16681 ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat
    16741 tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga
    16801 tatcgccatt aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcggcctt
    16861 cgtaccaacg agagtgcgga ttacgatgaa ttttgcgctt ctgttgcata aactgctcaa
    16921 ttcgctcatt cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt
    16981 actcattgaa cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat
    17041 aacgtccata acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta
    17101 aaagccgttt ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat
    17161 caccacggtt gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc
    17221 ttttttgcag caggcagaga ctgtcgcgca aagatgccgc ttctgtgcca gttcctgccg
    17281 gaacgtgagt tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat
    17341 tggcataagc gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac
    17401 gatcgccgag aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca
    17461 taggcagatc gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac
    17521 cggcatgatg acgtaaacgc tggctacccg gctgcgcacc cagcaatcgg tgcaaagtac
    17581 tggcatcttc cggaatacgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct
    17641 tgccgagaga ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac
    17701 ggcagcgttc gccgtcggcc atttgaatta acgctgccag caacttcgct acggtggtcg
    17761 ttttaccggt gccagggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg
    17821 ccgccacttt ttgccagtta atttcatcac cagtcggaaa gagtttatcc agggtttgcg
    17881 ccaacaaggc ttcatccacc tcgatggcat gattcacctc attgaagaag cgcgctaccg
    17941 tccgctcgtt acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg
    18001 tgggttcatc tccccggctg accgcttgtg aagccagcaa gcattcttcc caattttgta
    18061 gctcaccgat ttcactgaca caggtcgcca acagcggatg cgactcctca ttattttcca
    18121 gtcgtgaaag cggcaaacaa acgtgcccct ctccggcatc atgacttaac agtgccgccg
    18181 cgagggtgac ggcaggatgt tcatctcccg ccacggtcag ggcaaactgc acgtccagcg
    18241 ggcgtagctg tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc
    18301 ctccagagtc ataccggcaa acatctcatc catcaggtca atcaacccgg cgttggggcg
    18361 ggttgtgtaa atcccttgtt gcggatgttc tttatcaaca ccacgcagga acagataaat
    18421 aacgccgcca aagtggcgct catagtcgta atcagcaatg cgatggcgca gataacggtg
    18481 cagcgccagg gtataaagct gatattgcag atcatagcgg tgtgcctgca ttgccgctgc
    18541 catagcctgt tgggtgtaag ccgaactatc ttcacccaac cagttggatt tatagtcgag
    18601 caggtaataa cgcccttcgt ggcggaacac caggtcgata aagcctttta acatgccacg
    18661 tgcctgcatg aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag
    18721 tgcatcaagc tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac
    18781 ctgtttatcg cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg
    18841 gaggacagcc gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag
    18901 ttccagtttt tcctgtaccc agttcgggtc aaccggctgg gtaaaattga ggtcttcaaa
    18961 caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt
    19021 cggttcttca acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc
    19081 ctgagcgata ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc
    19141 gccgggcaat cgttgtagcg tcctcgcatt cagttctgca gttaaagcat cattaacctg
    19201 ccagggctgg ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc
    19261 ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc
    19321 gagggcactt tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg
    19381 tgcaacgccg agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag
    19441 atcttccgcc agacgttcga cctccgcaag atcgacgctt tctggcgcag cgttaagatc
    19501 cagaacagcc tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt
    19561 ggtaataaat ggcaaccaga ccagcggata ttccagccct tttgatttat ggatcgtgac
    19621 aatctgtacc agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc
    19681 tggctcgagg atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt
    19741 tccggcttct tgtagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc
    19801 tgccgttgcc agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcatcatcgg
    19861 cataacgcca cgtttgcgcc agatttgccg ataaccatcg aactcttcga ccaccgcgtc
    19921 ccacgcatgt tcgtcattat tcagcgtttc gatatccagc gcgttcagcc ccatcattga
    19981 cgttgccagc gcactacgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacaa
    20041 ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac
    20101 ggaagggatt tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg
    20161 caccagcaca ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa
    20221 cgcttcgccc cgctgtccgg cttgtagcca gtcgcgaatt tgcgcagcac ttacctgcgc
    20281 catggtactt tgatagtcgc caacgccgca actttcgcct tccatcagcc acattttcat
    20341 cgcaggctgc gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt
    20401 cactggaata aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt
    20461 gttcacactg ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc
    20521 gtgaacttca ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat
    20581 ggcctgcttc gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt gccagatacg
    20641 gcgaaaaatt cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg
    20701 gaatcgcgta cggatcgccg ctgccaacac ctcaccgctt tcgctacgca gcgcggaatc
    20761 gagccgactt aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac
    20821 tgtttcgcgg atctcagcca atgcgcgggt gatcaccaga tcgcggatcg acaatggttc
    20881 tgcaagcagt tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcctt
    20941 cgtgcgatct tctaagaaac gctgggagaa tttttccagc gactccggca actgataact
    21001 gtttctctct tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg
    21061 gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc
    21121 gtcgcgccac tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc
    21181 cagcgtttca tcatcgggcg gcggtgcttt gataaccggc gcttcacctt gcagataacg
    21241 attaatatcg cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat
    21301 ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtaacgtag
    21361 cagagactca tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag
    21421 gttgagcatg cgctggcaaa aaccgtgaat agtaaagact gccgcttcat ccatctgccg
    21481 ttcggctaac aacaaccact tcgcggcttg cgctttatcg tcgatctctt ccagcagacg
    21541 ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg atgcgcaact catggatatt
    21601 gctacggata cgaccacgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag
    21661 ttcttcaacg gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg
    21721 caaatagagc gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg
    21781 ctcgccctga aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt
    21841 cactcatcag gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt
    21901 caggatgcac atattcggct ttcccgttct ggctgccgga aatctgcgac agaatagcca
    21961 tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc aagtttttgc ggcgtcagca
    22021 gttttatctg ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat
    22081 ctttacttaa cttcaatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa
    22141 ttaccgcctg ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg
    22201 cggttgggaa aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt
    22261 gcaaaaggaa gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat
    22321 agcccaattg ttcttcagta cgcaactgat tgtagaacca cggctgtacg atctgcccca
    22381 acagagagct ataggctgag cttgtgtatt catcgtagcc agtcggtaca aataccgctg
    22441 ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga
    22501 ccactacatc tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat
    22561 cgcgtgccag cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc
    22621 gagcccctga ttttaaggcg tcacgataag ccagcacctc tttcaacgta atggagggca
    22681 aaattttacg ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca
    22741 taatcgcctg ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg
    22801 acttcgcctg ctcaagctga tcttccgtag cggtatagct aaagtatccc tcgagcaatg
    22861 cctggaacag ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt
    22921 tagcgttggt ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg
    22981 ccagccctgc gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg
    23041 ctttcggatt acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc
    23101 ttggcgcata caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt
    23161 tcttctctga cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg
    23221 agagcgcaat attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat
    23281 cgacctgata cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga
    23341 tataccagat acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag
    23401 catcgtaccg atcggcaata ttgactgcat ccagcgtatg ctcaacagga acgcgaatca
    23461 tggtatctgc cagccattcg acgtaatcca tatcacgggt gattgacgga taacggaagt
    23521 cgatatccag cacattcgcc agttcatcga agtattgttt atcgatccct ttttcacgta
    23581 acaaattgag gtagctaaaa atagccgcca caacctgatc gcgattcgcc agacctttat
    23641 cggttaaaga cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt
    23701 tggcgctaat gccctcaact aacccctgct tttgcagcca gtcagacagt gtacctgggc
    23761 tgcgattacc aatcagatag gtaatcaatt catccgtttt actgcggaac ttcgctgagt
    23821 tgttatcgat gcgaaactca acgcgcagaa ctttacgcgg cagcgctggg acgtaatgaa
    23881 tgataatgcc cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct
    23941 ctttgttcgg cacgcgacca aaagtgtccg ccgccatttt tgccaactcc ggcagcggtt
    24001 tattactgta aataaccgcc ttcatcaaat tggcggaata gtacttctcg tggaaatctt
    24061 tcagcgcctg ctgcaccgga ttaccaggtt tgtcgcttaa agtttcgagg ttaccaccag
    24121 aaaactttga accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca
    24181 tcccgtcacg tgtacgcgcc atggttaatt cagcgttcac tgcattacgc tcacgttcgg
    24241 cgtatttctt gtcgagcaag ggttctgcaa tagcatcggc caggcggtct accgcaccgg
    24301 gcaaggcgtc gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat
    24361 tgtgactacc gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt
    24421 tcgaccccat cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat
    24481 cttccagcga cccaacgggc accaccagcg ccgatagcga tttaactgcc tgcggatcag
    24541 aaaccagcaa gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt
    24601 tatcactttt acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg
    24661 gtgcccaaag ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattcacg
    24721 gacctcataa tcaactttat tttctggcca gattcaacag gttaacgcaa atcatctgcc
    24781 agaatttaat cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact
    24841 ggtgcgcatc ataaagtaag cggatagatt gcgcaatttt tatacagcgc tcatgactga
    24901 ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt caacgattgc ctccattgtc
    24961 tctggtgtta attgccgcca gagcctttga taccagatat catcaccttc gccactcacc
    25021 atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg
    25081 tcatccagca tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt
    25141 tcaggtaaca ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag
    25201 aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt
    25261 agaaaaaggc gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa
    25321 agttgcattc cctgcgccac acttaataaa gagggacgcc agcgcaacaa gccatccggc
    25381 tgcacctgcg gcaaccagcc agttatctgc acaccgttgc aagcgagatc aatttccata
    25441 ctctgccccg gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac
    25501 tgtgtttccc agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag
    25561 cggcggaaca agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg
    25621 atttgataac ggctaagtcc ttccagaata aatggctcgg tgtcggggat ttcgctgtct
    25681 tcagtacgga agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag
    25741 aatcgttgta gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca
    25801 aattcagaat gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt
    25861 tgtcgttcgc ctggctggta gttttgcgga tcaaacggca tccgggtatg gaggcaagta
    25921 agatgcgctt ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga
    25981 taatgacttt gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca
    26041 ctgttatcct gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt
    26101 aacgcttcca ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt
    26161 ttctggctca tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc
    26221 attcccagca ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg
    26281 ttaaccggcc cggcgagaaa acgctggctg atacgttctt gatccaggcg ctgtgccagt
    26341 tcatcacgca atagtgacag cggcaccgca tcgccatact gcgcacctaa accttcggcg
    26401 ataatcgcct gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc
    26461 aggaagaggg cgttgagcat atcgcgacaa accggcaacc actcttccag cggacgctcc
    26521 tgtgccagcc cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc
    26581 agttctgcaa ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc
    26641 gcgctctcca tcgcgtagcc caacaacata cgcgtcaggc caaatcgcca ggtgtgttgt
    26701 ccggtggcgg ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg
    26761 gattcgttga cccactgacg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc
    26821 gccagcaccg gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc
    26881 aacgataaca ggctgataaa cgcttcaagt acaggatgcg actgccgcgc ccgacggtcg
    26941 gaaatggcgt aaggcaggta acgatccgca ggtgcgctac caaacacagc ctgaataaac
    27001 ggactgtagc tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg
    27061 tcttcttcca gcatcgccag caggcgatcg tgtaaaactt caacttcacg ctgcgggcta
    27121 tggcaaacgt ggaaggtgat actgctatcc agtggatcaa gcggacgttt gttatcgcta
    27181 cgggaaaact cttcgatgtt cacaccagca acggcgcggt tttccagttc cagaatgtca
    27241 gactgaatgt tatgcagcag gttatctggc gtcacatcga caaaggcgtc cagctcctgg
    27301 ctgctctcca ggtcagaaag gagataaatg tagtcgcgcc caagcttgcc ccatgaggcc
    27361 agcagcgggt tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg
    27421 ctgtcgcgaa atagcggtaa ttcgcgatct tcaaaactgt gtcggcgctg gcgagtcagt
    27481 agtttcgcca gataagcagg atctttaatg tcgccccagt aataacggca ggggttggta
    27541 aacaggagat ggatttcaat atgtttaccc agcgcctgga gcgcctggag ataaacaggc
    27601 ggtaacgcgg aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc
    27661 gcggactcca gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgt
    27721 ccgagttcat gggtatattc caccagcgcc ttccacaacg gcgcttgcca ggcctgtgcc
    27781 tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag
    27841 accagatact ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ctttcgcttg
    27901 tcgctatcgt cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc
    27961 ggcagcagag tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg
    28021 atctccggta acacccggac gaacatatcc cagataaagc tcgcaggcag cggaaaatca
    28081 atgtttgccg caataccaaa cttttgcgac agggtcattt gcagccactg tgccataccg
    28141 gtactttgca ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca
    28201 ataaactcca tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata
    28261 gcggctcctg actactgacg attcgggcaa tgcaggcggg tcatctcgcc tgctctgccc
    28321 ccgggtgaaa ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg
    28381 ttgacctgcc agttggcagg tggcgaaatc gcgcgcaatt gcgtttgctg ccaaccatgc
    28441 cgccagagct gttggtactg gtttctgctg gtaagactgt tcattaatgt tcgctggaaa
    28501 cccgataacg cagtgacaat catcaccatc aacaccatcg ccaacattac ctccggcagg
    28561 ctaaagcctt gttgattctt cagggaagct gacataacgc cccctctttc agcggacaaa
    28621 aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc
    28681 acaacgaaac gccttcataa ccggcaatta ataaggcttc attatcttcc agtaaacgca
    28741 aacaaacccg ggcaccagtg gcagcgtaca gtaagcactg aactgcgggc tgcgtctgcc
    28801 aggagtgcat tttcccccac gccaacgccg actgaacgat ggcctggcgg cgcagtgact
    28861 gactttccat gctcacgcga gaggcaaaac tgcgatcctg ctggctcatt ccttgtaata
    28921 gcaagctacc caaaaccagc agcatcagga ccagagccag tgacgaaaca cccttttcgc
    28981 ggttcacagg ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga
    29041 cttactggca gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg
    29101 tacgacctga aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt
    29161 accttcacag gatgtcgcac cgcgtagcgt ttccaacact tgctccttca gacgaaatcc
    29221 aatctggtcg gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca
    29281 ctgcacaatg atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc
    29341 atgacaataa cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc
    29401 atcttccagt gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa
    29461 gcgtgcagcc cccaacaaca atacgctact gatcgccata gcaatcaaca cttccagcag
    29521 agaaaaacct tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt
    29581 cttccccacg gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gccaatatgc
    29641 ccggcccatg cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt
    29701 tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattcgcc
    29761 ccagcaacgg aactcacaag gcaccataac gtcccctccc tgataaaact gatactgtgg
    29821 tcgcggttat gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg
    29881 gcggtttgcc aaagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg
    29941 cttagcatga ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt
    30001 gttttcatgc cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct
    30061 tcggcgcatc ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg
    30121 tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg
    30181 atgtttcgta attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca
    30241 atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac
    30301 ttcggcagcg gacgcggttc acggcttaat tgcagatgag tttgatccat atgattactg
    30361 tacaggtgcg tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc
    30421 gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg
    30481 tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag
    30541 aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca
    30601 ataatgcggc gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca
    30661 atatgacgac catctggcgt tggccaggcg cgccactgtt taccatacac tggtccgagg
    30721 tcgccgtttt catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca
    30781 atgttagtgt cgccctgcag aaaccacagc agttcatgga tgatggaacg caggtggcaa
    30841 cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa
    30901 atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt
    30961 tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga
    31021 cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc
    31081 cccatgctga tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac
    31141 tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca agccagacac ggctcccatc
    31201 gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc
    31261 agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc
    31321 cactgcgggt tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg
    31381 gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaaa
    31441 cgcccggcac caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag
    31501 gaacgtttag tacggcgggc aaagataatc atcaccacga taacgccaat caggccgccg
    31561 tggaaagaca tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc
    31621 gggaaattgt agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc
    31681 gcatagagta agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt
    31741 cgtgttgcca gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga
    31801 agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta
    31861 ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg
    31921 cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg
    31981 ttgccggata ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc
    32041 ccgcccatgc cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt
    32101 tgcgcttcca gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc
    32161 aggtatttta cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca
    32221 atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt
    32281 ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga
    32341 ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc
    32401 agatcgttgg tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat
    32461 accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc
    32521 atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg
    32581 aggcttgtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc
    32641 atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc
    32701 caacccaggc atggattctc ttcgctaatc ggcatgtaag gcagctgctt atctgctccg
    32761 acatccagcg tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac
    32821 tgcgccacct gttcttcttc cgacggaaaa ccactttgca gcatgaatgg gatttcagtg
    32881 cgataaagac cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa
    32941 ccagcattga gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg
    33001 tcatcttccg ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc
    33061 agcagtaccg gctccggatc gaccagcaat tcaccgcgat agccatcgac gatcagcgtc
    33121 cgacgatgca gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca
    33181 cgtaccatga tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag
    33241 cgatcctggg gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa
    33301 cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga
    33361 cccagcgcac gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac
    33421 tgttcggcaa atttttcaat gaccgttttt accgcccatt ctgccaccga gcctttatca
    33481 acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg
    33541 aaaatagccg ccgtttcttt ttgtgcaccg gcggcaaagc gtttgctgta gcggcgaaac
    33601 tcgtttgccg cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc
    33661 gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca
    33721 atcgctacac caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac
    33781 aaggcagtca actgcgactg agaaagaatc gctgccatct gggtggcaag cgtcaccagg
    33841 aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca
    33901 agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt
    33961 acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc
    34021 gccagcctgc caaccaggcc gacgatccct tcatcaaagg cgagcgttac agtgcgaccg
    34081 cgtggttttt tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc
    34141 aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt
    34201 aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg
    34261 agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt
    34321 ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag
    34381 acatcacgtt taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag
    34441 ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc
    34501 tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc
    34561 aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct
    34621 ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct
    34681 ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac
    34741 attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca
    34801 tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt catactccag
    34861 gaagacggta aaccactctc tgcagggcat tacacactaa caacaattga ataactcaca
    34921 gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct
    34981 gtggatagag ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata
    35041 agcccgatat agacatttta aaatattcat tattagtcaa tgcattacat gtaatttaag
    35101 taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
    35161 tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
    35221 aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca
    35281 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
    35341 tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa aagcaatata
    35401 aatcagataa ttgaacaacg catgtggaaa aagttacact gcgaatattc ggcacataat
    35461 tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc
    35521 ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg
    35581 gagaactggc ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct
    35641 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgaacaag
    35701 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
    35761 tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggagtg acctgggaaa
    35821 ccagccacgt gcgccacaaa ctcaaacgcg tactgtggat accggttgaa ggcgagcgca
    35881 gcatcccgct ggcgcagcgt cgcgttggat caccgttgct gtggagcccg aatgaagagg
    35941 aagaccggca gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg
    36001 ttgagcggat taccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg
    36061 cgaaagcgct taccgaagcc attggtgcac ggggcgaacg gattctgacg ctgccgcgcg
    36121 gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt
    36181 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
    36241 ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac
    36301 gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
    36361 ggcaaagctc cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
    36421 ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga cgaatccgct
    36481 ttttacaata ttcagtcagg aaatttctgc ccgtgacttg attctgcttc tgggtggctt
    36541 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg
    36601 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
    36661 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
    36721 gatggccgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
    36781 ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
    36841 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
    36901 gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct
    36961 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac ttctgttacc ctcacagttt
    37021 aagattttcc tgctttcaag atatataacg tcagtttata aacagactat tatcataggt
    37081 gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
    37141 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
    37201 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
    37261 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaataat tgaacgttaa
    37321 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
    37381 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
    37441 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
    37501 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
    37561 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa
    37621 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
    37681 cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc
    37741 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgtctg cagactgatt
    37801 acctcgatct ttatcaggtg cactggccgc agcgcccaac caactgcttc ggcaaactcg
    37861 gttatagctg gacagattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg
    37921 cagagtacca acgagcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
    37981 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
    38041 agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
    38101 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat
    38161 atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
    38221 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg
    38281 gcctggcccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca
    38341 ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt ttgcatctgg
    38401 agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat acttatccgg
    38461 caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc gctgccagat
    38521 ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa tgggcacgac
    38581 cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt
    38641 ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgctc
    38701 ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc aacacgccaa tcagcatcaa
    38761 caaggcatag gctggcagct gctcgtgttg cagggagaaa atcagtacca ccacgccaat
    38821 caaaattccg gctggcatac agcgtgacac ggtttccagc gtaaccagct tcgctgccgc
    38881 acctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg gcgtagcgtt
    38941 atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc
    39001 cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag
    39061 cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg actgccccgg
    39121 acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac cgtaggccag
    39181 tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agcacaccac cggctacgga
    39241 accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt ttactaactt
    39301 actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc
    39361 aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac tggctgcgcc
    39421 cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc tatccgccac
    39481 ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt gcaggatggg
    39541 ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa acagtagggc
    39601 attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc ccttcgacca
    39661 caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccacttcg tctacccagc
    39721 ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt ttcaaatagc
    39781 gaatatcacg cggtacagca agctccggca cgccgtgctc acgggcatac tgttgcaact
    39841 tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct ttgctggcat
    39901 cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc agttgttcca
    39961 ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa
    40021 tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac cagccgcgct
    40081 ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc ttctccaccc
    40141 gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc tcttcgatac
    40201 cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc gtaccgggtt
    40261 tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca ccgtagcctt
    40321 caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct tgtagttttt
    40381 ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg aaacgcgcgt
    40441 agtgaccgag gaaagtcgaa gtgccgaaca acacggtgca actgcggtca tacaccaact
    40501 ccggcacaat acggtaatgc agcgggcttg gataaagaaa cacttctgca cctgtgagca
    40561 gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac gccgacataa
    40621 agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc gccagaatgc
    40681 ttttatggct atggacgacg ccttttggat ggccttcaga accggaggta aaaaggatca
    40741 gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc aaatgagcga
    40801 agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga tagacccagc
    40861 gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga aactggcggg
    40921 aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct tttaccccag
    40981 cagtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa atcactgccg
    41041 cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt
    41101 caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag tctggggtaa
    41161 aattgacgtc ttcgacacat ttcttccctg ctccgaagcg gtacattgca ctcagtaaag
    41221 attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt atttgatgca
    41281 gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc atctccacct
    41341 gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt
    41401 tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac
    41461 cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag cctgtggtgg
    41521 tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat
    41581 gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc
    41641 aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga cgcacaggta
    41701 aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta attagaacgc
    41761 gctcgccctt cagtgcctgg gggtcacccg taacgcgaac gcgatacaaa acacggcaca
    41821 aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa
    41881 tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa
    41941 cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac cgaataatct
    42001 caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt cgccagcgag
    42061 aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt tactgttaca
    42121 gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa ttcatgtgct
    42181 caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag caacctgaag
    42241 ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca aaggtttgca
    42301 atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg aaaacacgcc
    42361 gccccttgaa ccaacgggcg ttttccgtaa cactgaaaca atgtaagcgt ttacccacta
    42421 aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt cagtcgccac
    42481 cgtttcccgc gtcattaata attcacccaa agccagcgaa gcttcccggc tggctgtgca
    42541 tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc tggcgcagca
    42601 gaccactgaa acggtcggtc tggtcgttgg tgatgtttcc gatccgtttt tcggtgcaat
    42661 ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga ttggcaatgg
    42721 ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc atcgctgtgc
    42781 tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat tattgaaaca
    42841 aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc gttgtattgc
    42901 tctggacgat cgttacggtg cctggctggc tacgcgtcat ttaattcagc aaggtcatac
    42961 ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc gtctgcaagg
    43021 gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg tgacatttgg
    43081 cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac gaggaagaaa
    43141 tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg gcgttctcaa
    43201 tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg atgtgctggt
    43261 gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga cgatggcgac
    43321 ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg aaatcactaa
    43381 tgtctttagt ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc tggaggcaag
    43441 tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt aattcttcga
    43501 tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact tctggtaaca
    43561 gcggacggct attgtagttg gatgacattg atgcgccata tgcccctgta tcatgcagta
    43621 ccagataatc acctgccttc acttccggca aggcgcgggg ttcaacattt cccccttcct
    43681 gctgggtaaa gacatcgccc gattcacata acggtccggc gacgacggtt tccaccgttg
    43741 gtgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg taactaccgt
    43801 acattgccgg gcgcatcaga tcgttaaacc cggcatcaac cagcacaaag tggcggctac
    43861 ccatttgttt gacgctccgc acctgagtaa ttaacacgcc agactgcgct accaggaagc
    43921 gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt tgctcacgcg
    43981 cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc tgttgataag
    44041 gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat tcgatgacct
    44101 gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca gaaccaatgt
    44161 gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt gcggcgggca
    44221 gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt ttttggctat
    44281 gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc gaaacctggc
    44341 ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat tcactgacgc
    44401 gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg gggtgcgttt
    44461 gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg gaatccactt
    44521 tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct ttctgtgcaa
    44581 agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt tgcgcatcgt
    44641 agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg gcggtgagat
    44701 cggtatcggt gctgaacagt gaatgtggca taacaaactc cagatgagtg cttttttatg
    44761 attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct atgcaaaaat
    44821 gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta cgtcatattg
    44881 aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac ctgctacaca
    44941 cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc gggctgaaat
    45001 tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt ctgtttgaag
    45061 aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa agtctgcgcg
    45121 agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa tcttttttac
    45181 cgcagctcct gcaacccttt ctggtacgtt atcccgatgt cagcttaaat atcgtgcccc
    45241 aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta ggactcactg
    45301 agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta gatgaattgt
    45361 gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg gatgattttc
    45421 agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg ctggatcagc
    45481 tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc gccgcgtcag
    45541 tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc accgcactgg
    45601 attatgcggc aagcggttta gtggtgcggc ggttcagtat tgcggttccg ttcaccgtca
    45661 gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt agtgagcatt
    45721 tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg tcagctacga
    45781 cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca caggcaaaac
    45841 actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga cgccctgtgc
    45901 gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg cttcggtaaa
    45961 ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt catccgcttc
    46021 aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa aatcctgttc
    46081 catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg taattgcacg
    46141 tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa tggcatccgc
    46201 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
    46261 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
    46321 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
    46381 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
    46441 tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac cgcaacaagg
    46501 ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta ttaacgaaaa
    46561 aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt tctcaacttc
    46621 tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt ttccggaatg
    46681 agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca gaacgtaccg
    46741 gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat attcgacacc
    46801 cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg ctgaatttca
    46861 gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat acacatcatc
    46921 gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc aaactgcatc
    46981 aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat tttcagagcc
    47041 ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa cataaaggtc
    47101 agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc cgccattttg
    47161 aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg catcgcctgc
    47221 aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa cagtgcccaa
    47281 ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg cgctttttcc
    47341 gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc cttttccgcc
    47401 agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac tgctggtaaa
    47461 gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt atcggataaa
    47521 aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat cttaccgcga
    47581 acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc gatcccgaca
    47641 gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc aaaagcggac
    47701 cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt acgccccagg
    47761 cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat catgctacta
    47821 accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa cggcaacgct
    47881 ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc agctaccgaa
    47941 acaaacatat tcatacgccg cgtatcacgc aaagaacgtg gcgttaaagc agattccgta
    48001 ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa aatacgtgaa
    48061 caattcacac aggtgtcagg tcggaaacag cataaatatg gattaaattg ctgcgacatg
    48121 ccgttatgtg atggatattc caattttaaa attaagttga attattgaga tt
//
LOCUS       DAZGXS010000021        47502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGXS010000021 DAZGXS010000000
VERSION     DAZGXS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1801)
                     /gene="espL1"
                     /locus_tag="ACHKG5_001340"
     CDS             complement(<1..1801)
                     /gene="espL1"
                     /locus_tag="ACHKG5_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP0867478.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIH"
     gene            2324..4252
                     /gene="thrS"
                     /locus_tag="ACHKG5_001341"
     CDS             2324..4252
                     /gene="thrS"
                     /locus_tag="ACHKG5_001341"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0867479.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4256..4798
                     /gene="infC"
                     /locus_tag="ACHKG5_001342"
     CDS             4256..4798
                     /gene="infC"
                     /locus_tag="ACHKG5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0867480.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4895..5092
                     /gene="rpmI"
                     /locus_tag="ACHKG5_001343"
     CDS             4895..5092
                     /gene="rpmI"
                     /locus_tag="ACHKG5_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0867481.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5145..5501
                     /gene="rplT"
                     /locus_tag="ACHKG5_001344"
     CDS             5145..5501
                     /gene="rplT"
                     /locus_tag="ACHKG5_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0867482.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5618..5742
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5624..5668
                     /gene="pheM"
                     /locus_tag="ACHKG5_001345"
     CDS             5624..5668
                     /gene="pheM"
                     /locus_tag="ACHKG5_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0867483.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5951..6934
                     /gene="pheS"
                     /locus_tag="ACHKG5_001346"
     CDS             5951..6934
                     /gene="pheS"
                     /locus_tag="ACHKG5_001346"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0867484.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6949..9336
                     /gene="pheT"
                     /locus_tag="ACHKG5_001347"
     CDS             6949..9336
                     /gene="pheT"
                     /locus_tag="ACHKG5_001347"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0867485.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKASTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9341..9640
                     /gene="ihfA"
                     /locus_tag="ACHKG5_001348"
     CDS             9341..9640
                     /gene="ihfA"
                     /locus_tag="ACHKG5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0867486.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9741..10721
                     /gene="btuC"
                     /locus_tag="ACHKG5_001349"
     CDS             9741..10721
                     /gene="btuC"
                     /locus_tag="ACHKG5_001349"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0867487.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTFVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10784..11335
                     /gene="btuE"
                     /locus_tag="ACHKG5_001350"
     CDS             10784..11335
                     /gene="btuE"
                     /locus_tag="ACHKG5_001350"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0867488.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11335..12084
                     /gene="btuD"
                     /locus_tag="ACHKG5_001351"
     CDS             11335..12084
                     /gene="btuD"
                     /locus_tag="ACHKG5_001351"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0867489.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12162..12626
                     /gene="nlpC"
                     /locus_tag="ACHKG5_001352"
     CDS             12162..12626
                     /gene="nlpC"
                     /locus_tag="ACHKG5_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0867490.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12873..13586
                     /gene="ydiV"
                     /locus_tag="ACHKG5_001353"
     CDS             12873..13586
                     /gene="ydiV"
                     /locus_tag="ACHKG5_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0867491.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13649..15085
                     /gene="selO"
                     /locus_tag="ACHKG5_001354"
     CDS             13649..15085
                     /gene="selO"
                     /locus_tag="ACHKG5_001354"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0867492.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15089..15280)
                     /gene="hemP"
                     /locus_tag="ACHKG5_001355"
     CDS             complement(15089..15280)
                     /gene="hemP"
                     /locus_tag="ACHKG5_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0867493.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15412..16458)
                     /gene="aroH"
                     /locus_tag="ACHKG5_001356"
     CDS             complement(15412..16458)
                     /gene="aroH"
                     /locus_tag="ACHKG5_001356"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0867494.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16615..17448)
                     /gene="ppsR"
                     /locus_tag="ACHKG5_001357"
     CDS             complement(16615..17448)
                     /gene="ppsR"
                     /locus_tag="ACHKG5_001357"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0867495.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17780..20158
                     /gene="ppsA"
                     /locus_tag="ACHKG5_001358"
     CDS             17780..20158
                     /gene="ppsA"
                     /locus_tag="ACHKG5_001358"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0867496.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20215..21861)
                     /gene="fadK"
                     /locus_tag="ACHKG5_001359"
     CDS             complement(20215..21861)
                     /gene="fadK"
                     /locus_tag="ACHKG5_001359"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0867497.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21918..22211)
                     /gene="ydiT"
                     /locus_tag="ACHKG5_001360"
     CDS             complement(21918..22211)
                     /gene="ydiT"
                     /locus_tag="ACHKG5_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0867498.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22208..23497)
                     /gene="ydiS"
                     /locus_tag="ACHKG5_001361"
     CDS             complement(22208..23497)
                     /gene="ydiS"
                     /locus_tag="ACHKG5_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0867499.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23553..24491)
                     /gene="ydiR"
                     /locus_tag="ACHKG5_001362"
     CDS             complement(23553..24491)
                     /gene="ydiR"
                     /locus_tag="ACHKG5_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0867500.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24511..25275)
                     /gene="ydiQ"
                     /locus_tag="ACHKG5_001363"
     CDS             complement(24511..25275)
                     /gene="ydiQ"
                     /locus_tag="ACHKG5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0867501.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25591..26502
                     /gene="ydiP"
                     /locus_tag="ACHKG5_001364"
     CDS             25591..26502
                     /gene="ydiP"
                     /locus_tag="ACHKG5_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0867502.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYLKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(26545..27696)
                     /gene="ydiO"
                     /locus_tag="ACHKG5_001365"
     CDS             complement(26545..27696)
                     /gene="ydiO"
                     /locus_tag="ACHKG5_001365"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0867503.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(27710..29305)
                     /gene="ydiF"
                     /locus_tag="ACHKG5_001366"
     CDS             complement(27710..29305)
                     /gene="ydiF"
                     /locus_tag="ACHKG5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0867504.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(29450..30208)
                     /gene="aroD"
                     /locus_tag="ACHKG5_001367"
     CDS             complement(29450..30208)
                     /gene="aroD"
                     /locus_tag="ACHKG5_001367"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0867505.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(30239..31105)
                     /gene="ydiB"
                     /locus_tag="ACHKG5_001368"
     CDS             complement(30239..31105)
                     /gene="ydiB"
                     /locus_tag="ACHKG5_001368"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0867506.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(31117..32382)
                     /gene="ydiN"
                     /locus_tag="ACHKG5_001369"
     CDS             complement(31117..32382)
                     /gene="ydiN"
                     /locus_tag="ACHKG5_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867507.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(32609..33823)
                     /gene="ydiM"
                     /locus_tag="ACHKG5_001370"
     CDS             complement(32609..33823)
                     /gene="ydiM"
                     /locus_tag="ACHKG5_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867508.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(33923..34279)
                     /gene="ydiL"
                     /locus_tag="ACHKG5_001371"
     CDS             complement(33923..34279)
                     /gene="ydiL"
                     /locus_tag="ACHKG5_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0867509.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(34415..34522)
                     /gene="rprA"
                     /locus_tag="ACHKG5_001372"
     ncRNA           complement(34415..34522)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKG5_001372"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(34708..35820)
                     /gene="ydiK"
                     /locus_tag="ACHKG5_001373"
     CDS             complement(34708..35820)
                     /gene="ydiK"
                     /locus_tag="ACHKG5_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0867510.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            36209..39265
                     /gene="ydiJ"
                     /locus_tag="ACHKG5_001374"
     CDS             36209..39265
                     /gene="ydiJ"
                     /locus_tag="ACHKG5_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0867511.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            39262..39672
                     /gene="menI"
                     /locus_tag="ACHKG5_001375"
     CDS             39262..39672
                     /gene="menI"
                     /locus_tag="ACHKG5_001375"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0867512.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            39772..39960
                     /gene="ydiH"
                     /locus_tag="ACHKG5_001376"
     CDS             39772..39960
                     /gene="ydiH"
                     /locus_tag="ACHKG5_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0867513.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            40509..40877
                     /gene="sufA"
                     /locus_tag="ACHKG5_001377"
     CDS             40509..40877
                     /gene="sufA"
                     /locus_tag="ACHKG5_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0867514.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            40886..42373
                     /gene="sufB"
                     /locus_tag="ACHKG5_001378"
     CDS             40886..42373
                     /gene="sufB"
                     /locus_tag="ACHKG5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0867515.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            42383..43129
                     /gene="sufC"
                     /locus_tag="ACHKG5_001379"
     CDS             42383..43129
                     /gene="sufC"
                     /locus_tag="ACHKG5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0867516.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            43104..44375
                     /gene="sufD"
                     /locus_tag="ACHKG5_001380"
     CDS             43104..44375
                     /gene="sufD"
                     /locus_tag="ACHKG5_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0867517.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            44372..45592
                     /gene="sufS"
                     /locus_tag="ACHKG5_001381"
     CDS             44372..45592
                     /gene="sufS"
                     /locus_tag="ACHKG5_001381"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0867518.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            45605..46021
                     /gene="sufE"
                     /locus_tag="ACHKG5_001382"
     CDS             45605..46021
                     /gene="sufE"
                     /locus_tag="ACHKG5_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0867519.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            46170..47174
                     /gene="ldtE"
                     /locus_tag="ACHKG5_001383"
     CDS             46170..47174
                     /gene="ldtE"
                     /locus_tag="ACHKG5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0867520.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(47237..47473)
                     /gene="lpp"
                     /locus_tag="ACHKG5_001384"
     CDS             complement(47237..47473)
                     /gene="lpp"
                     /locus_tag="ACHKG5_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0867521.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT        12078 a        11433 c        12196 g        11795 t
ORIGIN      
        1 gatgaatggc tatatgaaca gctgacatgt tgtcatgatt tttagcagcc aacagtgtaa
       61 ataactgatg ttgactaaaa gaatgttttt ttgcaaaagc acccagcgtc gatatataag
      121 ataacacgac gtcttcatta cccttgctca tagcgatgta taatgcaggg gttccctgtg
      181 ctgtagcgcc ttttaataaa tccatgatgt tagctttgct caacttgtat ttaaaggcga
      241 tgccattaat tttagagagg aaccgtgtga cacacaaagg gtgattattt tccattgcgg
      301 cgtataatcc tggctcacca tcatgtattt tcatggctaa aatactcatc accagttttt
      361 tgtttaaaag ctcttgtgct ataggagtgg ttaatgcatt cagaaagata tcgattatct
      421 catcgaatcc atacaacatg gagatataaa gtccaggtgt ttcgttagca cttttagttt
      481 gcaacaattt aaccagatta tcttcatgaa tgatgtgatt atctaccagt gactgaattt
      541 cttgcataaa tattttcacg ttgtcagtat gtccgttcat catagccaaa aataaagcag
      601 gtgtaccaat gctattctgc gcagttatta tttcaatgat ttctttttca gggagtatgg
      661 gcaactgtct gaataatgta ataagctgtt gagtctggcc tgtggccatt attaacaaca
      721 ttgccgatgg atgtataggg gcaaatggca ttcgtgtcgc gatacccgtg aaaatagccc
      781 ctggaactcc ctcaggtaat aggggcagaa tagcaattgc acagttgcga acatacctat
      841 cagcccaggt ctgagcatcg ggacctgaac tgatgaactt atcaagcgag agaaagcccc
      901 gtgctgtcct gtgggtgcct ctgacggcaa cgttggtatc gttcgggtcg taaacggaca
      961 caacataatt taattgccca tacgccgact gctttattcg tagccttact gacaaagtat
     1021 gattgtctat atataaaatc gcagacatat gactgacatt gtttgccatc attcgacaga
     1081 agaaaatatc taaagcgaag ccaaaatcct tactcgcgat aacgcgactc cctgcaggag
     1141 gatggcgaag taatgctctg aactccgcgt cccggactaa ggggacatgc tgctgtatgg
     1201 attcaacgga ggagtaatgt ttaatcaggt tgaccttgcc ttttccattt ctcaatgaat
     1261 caagtgcgta ttgcaatgac agatggcgac aaaaaatagc gtcagaagga gattgcgaaa
     1321 aaaatgcact accattgaga tttactgttg gttgtatagt tttttctcta tggatttttt
     1381 tagacagttt catacaatgg ttacgatttt cggcagttaa ctgttcctgt tcaatataat
     1441 ccagtatgct tcgcaaatgg ttagctttaa taggcgtggt ttccagtaat aaggaaatga
     1501 tttctttttt atagtgtaat gtaatgaata tattttctgc atggatgggt gaagtggcaa
     1561 agttaatggt aaaaagggca gtggaataac aattttgtga tatttttcga cagtttgcgg
     1621 taaattcctg agtaacacag tatgacagca tcgaacttaa gtcagaggta tcaagtaggg
     1681 tgatgagttg gggcgcaaat tgattaatag gctctgatga aggcgttggt atgtcgctat
     1741 cgatgtgcat tctatgagga gacttataat gagttctgat aataatgtcg ttttgactca
     1801 taatctattc gccttatata tgtctggagg ttttgagtat gatgattaat tattatcgcc
     1861 agaacgaaat gtgcacgaaa aattgctata agaattgtgt cacttttatc attgtgatat
     1921 gaccacttcg tcattaatct tatgatcgtt taaatagtac cgccaaatca cgcaacgaac
     1981 tttgctggat tcagtttatg ctgctgtaaa ttcgctcgca gtaaaccttt cagacgctcc
     2041 gtgatgttat cagttgttct ttaagcgttt tgctggtgta ctcactacaa acgaattgcg
     2101 aatcgaatca atgtgaaacg gaaaggtaca atctcccttt ttttagtgtt aacatcgctc
     2161 aaccgggtgg aaggtgagag gttaatgtct ttcatttgtg tacctgattg cgaaccaatt
     2221 tagcatttgt tggctagatg gtttcgcaat gaactgttaa taaacaaatt tttctttgta
     2281 tgtgatcttt cgtgtgggtc accactgcaa ataaggatat aaaatgcctg ttataactct
     2341 tcctgatggc agtcaacgcc attacgacca cgctgtaagc cccatggatg ttgcgctgga
     2401 cattggtcca ggtctggcga aagcctgtat cgcagggcgc gttaatggcg aactggttga
     2461 tgcttgcgat ctgattgaaa acgacgcaca actgtcgatc attaccgcca aagacgaaga
     2521 aggtctggag atcattcgtc actcctgtgc gcacctgtta gggcacgcga ttaaacaact
     2581 ttggccgcat accaaaatgg caatcggccc ggttattgac aacggttttt attacgacgt
     2641 tgatcttgac cgcacgttaa cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga
     2701 gcttgctgag aaaaactacg acgtcattaa gaagaaagtc agctggcacg aagcgcgtga
     2761 aactttcgcc aaccgtgggg agagctacaa agtctccatt cttgacgaaa acatcgccca
     2821 tgatgacaag ccaggtctgt acttccatga agaatatgtc gatatgtgcc gcggtccgca
     2881 cgtaccgaac atgcgtttct gccatcattt caaactaatg aaaacggcag gggcttactg
     2941 gcgtggcgac agcaacaaca aaatgttgca acgtatttac ggtacggcgt gggcagacaa
     3001 aaaagcgctt aacgcttacc tgcagcgcct ggaagaagcc gcgaaacgcg accaccgtaa
     3061 aatcggtaaa cagctcgacc tgtaccatat gcaggaagaa gcgccgggta tggtattctg
     3121 gcacaacgac ggctggacca tcttccgtga actggaagtg tttgttcgtt ctaaactgaa
     3181 agagtaccag tatcaggaag ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa
     3241 aaccggtcac tgggacaact acaaagatgc aatgttcacc acgtcttctg agaaccgtga
     3301 atactgcatt aagccgatga actgcccggg tcacgtacaa attttcaacc aggggctgaa
     3361 gtcttatcgc gatctgccgc tgcgtatggc cgagtttggt agctgccacc gtaacgagcc
     3421 gtcaggttct ctgcatggcc tgatgcgcgt gcgtggattc acccaggatg acgcgcatat
     3481 cttctgtact gaagaacaaa ttcgcgatga agttaacgga tgtatccgtt tagtctatga
     3541 tatgtacagc acttttggct tcgagaagat cgtcgtcaaa ctctccactc gtcctgaaaa
     3601 gcgtattggc agcgacgaaa tgtgggatcg tgctgaggcg gacctggcgg ttgcgctgga
     3661 agaaaacaac atcccgtttg aatatcaact gggtgaaggc gctttctacg gtccgaaaat
     3721 tgaatttacc ctgtatgact gcctcgatcg tgcatggcag tgcggtacag tacagctgga
     3781 cttctccttg ccgtctcgtc tgagcgcctc ctatgtgggc gaagacaacg agcgtaaggt
     3841 accggtaatg attcaccgcg caattctggg gtcgatggaa cgtttcatcg gtatcctgac
     3901 cgaagaattc gctggtttct tcccgacctg gcttgcgcca gttcaggttg ttatcatgaa
     3961 tattaccgat tcacagtctg aatacgttaa cgaattgacg caaaaactat caaatgcggg
     4021 cattcgtgtt aaagcagact tgagaaatga gaagattggc tttaaaatcc gcgagcacac
     4081 tttgcgtcgc gtcccgtata tgctggtctg tggtgataaa gaggtggaat caggcaaaat
     4141 tgccgttcgc acccgccgtg gtaaagacct gggaagcatg gacgtaaatg aagtgatcga
     4201 gaagctgcaa caagagattc gcagccgcag tcttaaacaa ttggaggaat aaggtattaa
     4261 aggcggaaaa cgagttcaaa cggcgcgccc taaccgtatc aatggcgaaa ttcgcgccca
     4321 ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt attgtgagtc tgagagaagc
     4381 tctggagaaa gcagaagaag ccggagtaga cttagtcgag atcagcccta acgccgagcc
     4441 gccggtttgt cgtataatgg attacggcaa attcctctat gaaaagagca agtcttctaa
     4501 ggaacagaag aaaaagcaaa aagttatcca ggttaaggaa attaaattcc gtcctggtac
     4561 agatgaaggc gactatcagg taaaactccg cagcctgatt cgctttctcg aagagggtga
     4621 taaagccaaa atcacgctgc gtttccgcgg tcgtgagatg gcgcaccagc aaatcggtat
     4681 ggaagtgctt aatcgcgtga aagacgattt gcaagaactg gcagtggtcg aatccttccc
     4741 aacgaagatc gaaggccgcc agatgatcat ggtgctcgct cctaagaaga aacagtaagg
     4801 ccttcaagta gcactgtctg tggagccttc gggcttcaca ggctttgttc gcctttgttt
     4861 cgtttattaa caatgcgaag tggaagttat taaaatgcca aaaattaaga ccgtacgcgg
     4921 tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt aagcacaagc acgctaacct
     4981 gcgtcacatt ctgaccaaaa aagcgaccaa acgtaaacgt cacctgcgtc cgaaagccat
     5041 ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg ccgtacgcat aagccgttaa
     5101 cgtttttaac tttttaatta gaatatagat acaggagagc acatatggct cgcgtaaaac
     5161 gtggtgttat tgcacgcgca cgtcacaaga aaattttgaa acaagctaaa ggctactacg
     5221 gtgcgcgttc tcgcgtatac cgcgttgcct tccaggctgt tatcaaagct ggtcagtatg
     5281 cttaccgtga ccgtcgtcaa cgtaagcgtc agttccgtca actgtggatt gcgcgtatca
     5341 acgcagcagc acgtcagaac ggtatttctt acagcaaatt catcaatggc ctgaaaaaag
     5401 cctctgttga aatcgaccgt aagatcctgg ctgatatcgc agtattcgac aaagtagcgt
     5461 tcaccgctct ggttgaaaaa gcgaaagcag ctctggcata agccagttga aagagggagc
     5521 tagtctccct cttttcgttt caacgccatc aaaacattga cttttatcgc cgtagccttt
     5581 tcaataaagg tcttttgaag agtaccaaaa ggtaacgcaa gcaatgaatg ctgctatttt
     5641 ccgcttcttt ttttacttta gcacctgaat ccaggaggct agcgcgtgag aagagaaacg
     5701 gaaaacagcg cctgaaagcc tcccagtgga ggcttttttt gtatgcgcgt tttgaaaaat
     5761 aaaaagccag tctatcaggc gtaattgtcg cagccagtct ggatacggcc agtgcggaga
     5821 aaccggagcg tacttaagta cgtgaggatt tcgagcactg cccgggacca aaatggcaag
     5881 taaaatagcc tgatgggata ggctctaagt ccaacgaacc agtgtcacca ctgacacaat
     5941 gaggaaaacc atgtcacatc tcgcagaact ggttgccagt gcgaaggcgg ccattagcca
     6001 ggcgtcagat gttgccgcgt tagacaatgt gcgcgtcgaa tatttgggta aaaaagggca
     6061 cttaaccctt cagatgacga ccctgcgtga gctgccgcca gaagagcgtc cggcagccgg
     6121 tgcggttatc aacgaagcga aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga
     6181 actggaaagc gctgcactga atgcgcgtct ggcggcggaa acgattgatg tctctctgcc
     6241 aggtcgtcgc attgaaaacg gcggtctgca tccggttacc cgtaccatcg accgtatcga
     6301 aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg ccggaaatcg aagacgatta
     6361 tcataacttc gatgctctga acattcctgg tcaccacccg gcgcgcgctg accacgacac
     6421 tttctggttt gacgctaccc gcctgctgcg tacccagacc tctggcgtac agatccgcac
     6481 catgaaagcc cagcagccac cgattcgtat catcgcgcct ggccgtgttt atcgtaacga
     6541 ctacgaccag actcacacgc cgatgttcca tcagatggaa ggtctgattg ttgataccaa
     6601 catcagcttt accaacctga aaggcacgct gcacgacttc ctgcgtaact tctttgagga
     6661 agatttgcag attcgcttcc gtccttccta cttcccgttt accgaacctt ctgcagaagt
     6721 ggatgtcatg ggtaaaaacg gtaaatggct ggaagtactg ggctgcggga tggtgcatcc
     6781 gaacgtgctg cgtaacgttg gcatcgaccc ggaagtttac tctggtttcg ccttcgggat
     6841 ggggatggag cgtctgacta tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga
     6901 aaacgatctg cgtttcctca aacagtttaa ataaggcagg aatagattat gaaattcagt
     6961 gaactgtggt tacgcgaatg ggtgaacccg gcgattgata gcgatgcgct ggcgaatcaa
     7021 atcactatgg cgggcctgga agttgacggt gtagaaccgg ttgccggtag cttccacggc
     7081 gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg
     7141 acaaaagtga atgtcggcgg cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc
     7201 cgtcagggcc tgcgtgtggc ggtagcgacc attggtgctg ttctgccggg tgatttcaaa
     7261 attaaagcgg cgaaactgcg cggcgaaccg tctgaaggga tgctgtgctc cttttctgag
     7321 ctgggaattt ctgacgacca taacggcatt atcgaactgc ctgcagatgc gccgattggc
     7381 actgacatcc gcgaatacct gaaactcgat gacaacacca tcgaaatcag cgtaacgcca
     7441 aaccgtgccg actgtttagg tatcattggt gttgcgcgtg acgttgccgt gctgaatcag
     7501 ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga ccatcgacga cacgctgccg
     7561 attacagtcg aagcgccgga agcctgcccg cgttatcttg gccgtgtggt aaaaggcatt
     7621 aacgttaaag cgtcaactcc gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt
     7681 tctatcgatg cagttgttga cgtcaccaac tatgtgctgc tcgaattggg ccagccgatg
     7741 cacgctttcg ataaagatcg cattgaaggc ggcattgtgg tgcggatggc gaaagagggc
     7801 gaaacgctgg tgctgctcga cggtactgaa gcgaagctga atgctgacac tctggtcatc
     7861 gccgaccaca acaaggcgct ggcgatgggc ggcatcttcg gtggcgaaca ctctggcgtg
     7921 aatgacgaaa cacaaaacgt gctgctggaa tgcgctttct ttagcccgct gtctatcacc
     7981 ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc accgttatga gcgtggcgtt
     8041 gatccggcac tgcagtacaa agcgatggaa cgtgcgaccc gtctgctgat tgacatctgc
     8101 ggtggtgagg ctggtccggt aattgatatc accaacgaag caacgctgcc gaagcgtgca
     8161 accatcactt tacgtcgtag caaactggat cgcctgatcg gccatcatat tgcggatgag
     8221 caggtaactg acattctgcg tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg
     8281 caggcagttg cgccgagctg gcgtttcgac atggagattg aagaagatct ggtcgaagaa
     8341 gtcgcgcgtg tttacggcta caacaacatc ccggatgagc cggtacaggc aagcctgatt
     8401 atgggtactc accgtgaagc tgacctgtcg ctcaagcgcg tgaaaacgct gctcaacgat
     8461 aaaggctatc aggaagtgat cacctatagc ttcgttgatc cgaaagtgca gcagatgatc
     8521 catccaggcg ttgaagcctt actgctgcca agcccgatct ctgttgaaat gtcagcaatg
     8581 cgtctttctc tgtggaccgg cctgctggca accgtggtgt acaaccagaa ccgtcagcag
     8641 aaccgtgtgc gcattttcga aagcggtctg cgtttcgtac cagatactca ggcaccgttg
     8701 ggcattcgtc aggatctgat gttagccggt gtgatttgcg gtaaccgtta cgaagagcac
     8761 tggaacctgg caaaagagac cgttgatttc tatgatttga aaggcgatct tgaatccgtt
     8821 ctcgacctga ccggtaaact gaatgaggtt gagttccgtg cagaagcgaa tccggcactg
     8881 catccggggc aatccgcagc gatttatctg aaaggtgaac gtattggttt tgttggggtt
     8941 gttcatcctg aactggaacg taaactggat cttaacggtc gcactctggt gttcgaactg
     9001 gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg
     9061 gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta
     9121 tccgaatgta agaaagttgg cgtaaatcag gtagttggcg taaacttatt tgacgtgtac
     9181 cgcggtaagg gtgttgcgga ggggtataag agcctcgcca taagcctgat cctgcaagat
     9241 accagccgta cactcgaaga agaggagatt gccgctaccg tcgccaaatg tgtagaggca
     9301 ttaaaagagc gattccaggc atcattgagg gattgaacct atggcgctta caaaagctga
     9361 aatgtcagaa tatctgtttg ataagcttgg gcttagcaag cgggatgcca aagaactggt
     9421 tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc
     9481 tggttttggt aacttcgatc tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac
     9541 gggcgaggat attcccatta cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt
     9601 aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc
     9661 cgctctgcgg ccttttttct tttcactgtc gaagagtcac cgtaaaatca acgccatgac
     9721 acttcagcag aacggatacc atgctgacac ttgcccgcca acaacagcga caaaatattc
     9781 gctggttatt atgcctgtca gttttgatgc tgctggcgct tctcttaagc ctttgcgccg
     9841 gtgaacaatg gatctcgcca ggtgactggt tttctcctcg tggcgaactg ttcgtctggc
     9901 aaattcgcct gccacgtacg ctggctgtat tgctggttgg tgcggcgctg gctatatccg
     9961 gcgctgtaat gcaggcgttg tttgaaaatc ctctggcaga acctggacta cttggcgtct
    10021 ctaacggcgc aggcgtgggg cttatcgccg cggtattgct tgggcaaggg caactcccca
    10081 actgggcgct agggctgtgt gcgattgctg gggcgcttat catcacttta atactcttac
    10141 gtttcgcccg tcgtcatctt tcgaccagtc ggttattgct ggctggcgtt gcattaggga
    10201 ttatctgtag cgcactaatg acgtgggcta tctacttttc cacctttgtt gatttacgtc
    10261 agctgatgta ctggatgatg ggcggttttg gcggcgtaga ctggcggcaa agctggctga
    10321 tgctggcatt gatccccatg ttgttgtgga tctgttgtca gtccaggccg atgaatatgt
    10381 tagcacttgg cgagatctcg gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg
    10441 tgctggtggc agcgaccggc tggatggttg gcgtcagtgt agcgctggcg ggtgctatcg
    10501 gctttattgg tctggtgatc ccacatattc ttcggttgtg tggtttaacc gatcatcgcg
    10561 cattacttcc cggctgcgcg ctggcagggg cgagcgcatt gctgctggcc gatattgtag
    10621 cgcgcctggc attagctgcc gcagagctgc ctattggcgt ggtcaccgca acgttgggtg
    10681 cgccggtgtt tatctggtta ttgttaaaag caggacgtta gccgcaaaaa gacggtctat
    10741 gattaaaagc taagattttt actgaccaca cccaggagaa acgatgcaag attccattct
    10801 gacgaccgta gtgaaagata tcgacggtga agtgaccacg ctggagaagt acgccggtaa
    10861 tgtgctgttg attgtcaatg tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt
    10921 ggagaatatt cagaaagcct gggccgatcg aggttttgtg gtgctgggat tcccgtgcaa
    10981 ccagtttctg gaacaagaac cgggcagcga tgaagagatt aaaacttact gtaccaccac
    11041 atggggggtg acgttcccga tgttcagtaa gattgaagtt aatggcgaag gacgccatcc
    11101 gctgtatcaa aaattgattt ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt
    11161 ctatgcccgt atggtcagca aaggtcgtgc accgctgtac cctgatgata ttttatggaa
    11221 ttttgagaaa tttttagttg gcagggacgg attggtcatc cagcgttttt ccccggatat
    11281 gacgccggaa gatcccattg tgatggaaag cattaaactg gcgttggcaa aataatgtct
    11341 attgtgatgc agttacagga tgttgcggaa tctacccgcc tggggccgct ttctggcgag
    11401 gttcgggctg gggagatcct gcacctggtg gggccgaatg gcgcgggtaa gagtacctta
    11461 ctggcgcgaa tggccggaat gaccagcggt aagggaagca ttcagttcgc ggggcaacca
    11521 ctggaagcat ggtccgcaac aaaactcgcg ctgcatcgtg cctatctttc acaacagcag
    11581 acgccgccgt ttgcaatgcc ggtctggcac tacctgacac tgcatcagca cgataaaacg
    11641 cgtaccgaac tactgaatga tgtcgcaggg gcgctggctc ttgatgacaa actcggtcgt
    11701 agcaccaatc aactttccgg cggtgaatgg caacgcgtac gtcttgctgc ggtggtgctg
    11761 caaatcacac cacaagccaa tcccgcaggc caattgctgc ttcttgatga gccgatgaac
    11821 agtcttgatg ttgcgcaaca aagtgcgtta gacaaaattc tgagcgcgct gtgtcagcaa
    11881 ggactggcga ttgtgatgag cagtcacgat ctcaaccaca cattgcgtca tgcgcatcgg
    11941 gcgtggttgc taaaaggtgg aaaaatgctg gccagtggac gcagagaaga tgtgctcacg
    12001 ccgccaaatc tggcgcaggc ctatgggatg aattttcgcc gtctggatat cgaaggtcac
    12061 agaatgctga tttcgaccat ctgatttcat tagtatcttg aaaatcgggt aattacgacg
    12121 ctaaattaat gcaagaataa aaaacagagg aaccgtacgg aatgcgtttc tgccttattt
    12181 tgatcacagc actgtttctg gccgggtgta gccaccataa agcaccgccg ccaaatgcca
    12241 gactttctga ttcgattacc gttattgccg gtttgaacga ccagctacaa agctggcatg
    12301 gcacgccgta tcgttatggt ggcatgacgc ggcgcggtgt ggactgttcg ggatttgtgg
    12361 ttgtgacgat gcgcgatcgt ttcgatttgc agctgccccg agaaaccaaa gaacaagcct
    12421 ctatcggcac gcaaattgat aaagacgagt tgctgcctgg tgacctggtc tttttcaaaa
    12481 cgggttccgg acaaaatggt ttgcatgtag gtatttacga taccaacaac caatttatcc
    12541 acgcctctac cagcaaggga gtgatgcgtt cctcacttga taatgtctat tggcagaaaa
    12601 atttctggca ggcgagacga atctagtatg tcgaaaattg gacgtcgatg atacatcgcc
    12661 gtccatttac tccagaatac agtagtattt tgcagacaaa aattccgtaa atggattatt
    12721 cgtttataat caggataatc taaaaaaagg acccctgaaa aagtggttaa catggaacaa
    12781 atgaaagtta ctccttctgt aattgttatg tcttatggcc tcatcccttt tagccggata
    12841 ctgaaaaaca tccttcgaga gggacggtta ccatgaagat ttttttggag aatctttatc
    12901 attctgattg ctatttcctg ccgataagag ataaccagca agtccttgtc ggcgttgagt
    12961 taattaccca tttctccagt gaagatggca cggtgcgtat cccgacgagt cgggttatag
    13021 cgcaacttac cgaggagcaa cattggcaat tattttcaga gcagttggaa ttactaaaat
    13081 catgccaaca tttttttatt caacataaac tctttgcctg gcttaattta acgccacaag
    13141 tggctacatt attattagag agggataatt acgcaggcga actattaaaa tatccgttta
    13201 ttgaactgtt gattaatgag aattacccgc atctcaatga aggaaaagat aatcgcggtc
    13261 ttttatcctt atcgcaagtg tatccccttg tgttaggtaa tttgggagca ggcaatagca
    13321 caatgaaagc ggtttttgat ggtcttttca cccgcgtaat gctggataaa agttttattc
    13381 agcaacaaat cacgcatcgc tccttcgaac cgtttattcg tgcgattcag gcgcaaattt
    13441 ccccttgttg caattgcatc atcgcggggg gcattgatac gccagaaata ttagcacaga
    13501 ttaccccctt cgatttccat gctctccagg gatgcctgtg gcctgccgtt cccataaatc
    13561 agataacgac gttggttcag cgataaccct cctgtttgct ggtgtttaag acgagagtaa
    13621 ccgtctacac tatcaaacag gaggctctat gaccctgtct tttattaccc gctggcgcga
    13681 tgaattgcca gaaacctata cagcactttc ccctacgcct ttaaataatg cccggctgat
    13741 ttggcataat accgaactcg ctaacacgct gagtattcca tcgtcgctgt ttaaaaatgg
    13801 cgcaggtgtc tggggcggcg aaaccctact gcctggtatg tcaccactgg cgcaggttta
    13861 cagtggtcat cagttcggcg tctgggcggg ccaactgggt gatgggcgcg gcattttact
    13921 tggcgaacaa ctgcttgctg atggcactac aatggactgg catctgaaag gtgctggcct
    13981 gacgccttat tcgcgaatgg gtgatggacg ggcggtatta cgttcgacga tacgagaaag
    14041 tctcgccagt gaggcgatgc attatctggg cattccgacg acccgcgcgt taagtatcgt
    14101 caccagcgat tcgccagtgt atcgggaaac ggtggagcca ggcgcgatgc tgatgcgtgt
    14161 ggcaccaagt catctgcgct ttggtcattt cgaacatttt tactatcgcc gcgagccgga
    14221 taaggttcgt cagttggctg actttgccat tcgtcattac tggtcacatc ttgaagatga
    14281 tgaggacaaa taccgtctct ggtttaacga tgttgtcgca cgtaccgcat cgttaattgc
    14341 ccaatggcag acggtcggct ttgcacatgg ggtgatgaat accgacaaca tgtcgctgct
    14401 gggactgacg cttgattacg ggccgtttgg ttttcttaat gattacgaac ccggttttat
    14461 ttgtaatcac tcggatcatc aaggacgtta cagctttgac aatcaacctg ctgtcgcgtt
    14521 gtggatttta cagcgtctgg cgcagacatt gtcaccattt gttgccgtag atgccctgaa
    14581 tgaggccctg gacagctatc agcaggtttt gttgacgcat tatggacaac ggatgcggca
    14641 gaaactgggc tttatgacgg agcaaaaaga ggataacgcg ctactgaatg aattattcag
    14701 tctgatggcg cgagagcgca gcgattatac ccgcacattt cgcatgctga gtctgaccga
    14761 gcagcacagc gcggcgtcac cgctacgtga tgagtttatt gatcgtgcgg catttgatga
    14821 ctggtttgcc cgttatcgag ggcgtttgca acaagacgag gttagcgata gtgagcgtca
    14881 gcaactgatg caaagcgtta accccgctct ggtattgcgc aactggttgg cgcaacgggc
    14941 gattgaagcg gcagaaaagg gtgatatgac ggaattgcac cgcctgcatg aggcgttgcg
    15001 aaatcctttc agcgacagag atgatgacta tgtcagtcgt ccacctgact ggggtaaacg
    15061 gctggaagtc agctgctcga gttaaaggct acttggtcaa cagcagcttg ccagcctgag
    15121 ttttacggag cagatattct tgcccgtcat gatcgataat cagtttgcca tccggaccta
    15181 acagtgtctg gctggaaatt cgccgaatag gctgcgggga agcggtcttg tgattggcat
    15241 ccgtttcagg cgtgagtttt ctgctatccg tataacgcat aaaaattaat aataattgat
    15301 ataacaatga ttcttattat caatattatg gctgaccaga gcaagcagta atgtgttgag
    15361 aagacaacaa gttgcgcaga aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg
    15421 ggtatctacc gcagaggcta gtttttcgac caggcgttcg gtatcttccc agcccagaca
    15481 cgggtcggta atggattgac cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc
    15541 ttcgcgcagg aaactttccg ccataattcc agcaatcgcc gtagagccat tgcggatttg
    15601 ctgacaaata tcctcacaaa cttctaactg gcgacggtgc tgcttctggc agttaccgtg
    15661 gctgaaatcc accaccagat gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc
    15721 ggcgatatca tcggcatgat aattcggttt tttgccgcca cgcataataa tgtggccata
    15781 cgggttgccg ctggtctgat agatggtcat ctgaccattt ttgtctggcg agaggaacat
    15841 atggctggcg cgggctgcgc ggatagcatc cacagcaatc cgcgtattgc catcggtacc
    15901 atttttaaaa cctaccggac aggagagtgc cgaagccatt tcacggtgga tctgactttc
    15961 ggtagtacgt gcgccaatcg cgccccaact gattaaatca gcaataaact gaccggtcac
    16021 catatcgagg aactcagtcg cggttgggac gcccagctca tttacctgta aaagtaattt
    16081 gcgcgccagc tccagaccgt gatttatccg atagctgccg tttaaatctg gatcggagat
    16141 tagtcctttc cagccgacaa cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc
    16201 cagccgtgac tggtactggt tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc
    16261 ggtgagatcg tggatcgagc aggggccaat aatgaccaac agtcgcttat cttcaccatt
    16321 gagtattttt tcaattctgc ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac
    16381 gggataccgt agcgcgagtt cggcgggcgt taccaggctc tcaatacgcg cagtacggag
    16441 ttcgtcagtt ctgttcatta caagtctcag aaatttgttg cttctacggc aggactgccg
    16501 ggaagtgatg cgcatcacga taaaccaatc gctgctgatt tcaagtgcga ggtgtgtcac
    16561 acctcgccgg gtggttagca tgataacaaa aaaataagct aatgcactag ttctctagta
    16621 cattcggcga ctaaggccca tgatatcgag gatcttggtg gcaatctctt ctaccgaata
    16681 attggtactg ttaatccacg ggatctgatt tttacggtac aaggcttcca cttccgcgac
    16741 ttccatcctg cactgacgaa gcgaggcata gcgactgttc tcccgacgtt cctcgcgaat
    16801 cgccgccaga cgttccgggt cgatagtcag gccgaacaat ttatgctgaa gcggtttgag
    16861 cgacgcgggt agcaccagat tatccatatc gtcggcaata aaggggtagt ttgccgcgcg
    16921 gataccaaat tgcattgcca gatacagact ggtgggggtt ttaccacagc gagaaacacc
    16981 gagcaggatc acctgagcct gatccagatt gcgcaacgaa atgccgtcat cgtgggcgag
    17041 ggtgtaatca atcgccgcaa tgcgcgcatc atatttattg agattattag ggttaaggcc
    17101 atgggtacga tgagcaatcg gcgttggatc cagtttcatc tcttgttgta gcggggcaac
    17161 cagcgcctga acgatatcct ggcaaaaacc ttcactttgc aagatgatgg cgcgaatttc
    17221 cggcaacacg atggagtaga agaccagcgg acgcacgcct gtctggtgat aaattgcgtc
    17281 aatctgatcc ttcactgccc gtgcacggct ctcattttcg acaaacggca gcgtgatgct
    17341 gctgatagtg acgggaaatt gtgacattac tgcgtgtcct aatacctccg cagttattgc
    17401 cgtaccatca gaaatataaa aaacgtggcg atcaacagca ttatccattt tgttcttccc
    17461 gtgatgcaga cataattcta tgaaagcata aattaaaaac gcacagaagc gtagaacgtt
    17521 atgtctggtt tataaaatga accttcaatt ttatttttta tgaaaacagc atttcatttt
    17581 tatggttcgt ttataccgat ggtttatgtg gaaattgtcg aaaagagcag atttgcgcaa
    17641 cgctgggatc agtcttaaca agtaaaaaaa tatatttgct tgaacgattc accgtttttt
    17701 tcatccggtt aaatatgcaa agataaatgc gcagaaatgt gtttctcaaa ccgttcattt
    17761 atcacaaaag gattgttcga tgtccaacaa tggctcgtca ccgctggtgc tttggtataa
    17821 ccaactcggc atgaaggatg tagacagggt tgggggcaaa aatgcctccc tgggtgaaat
    17881 gattactaac ctttccggaa tgggtgtttc cgttccgaat ggtttcgcca caaccgccga
    17941 cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag cgcatttatg aactgctgga
    18001 taaaacggat attgacgatg ttactcagct tgcgaaagcg ggcgcgcaaa tccgccagtg
    18061 gattatcgac actcccttcc agcctgagct ggaaaacgcc atccgcgaag cctatgcaca
    18121 gctttccgcc gatgacgaaa acgcctcttt tgcggtgcgc tcctccgcca ccgcagaaga
    18181 tatgccggac gcttcttttg ccggtcagca ggaaaccttc ctcaacgttc agggttttga
    18241 cgccgttctc gtggcagtga aacatgtatt tgcttctctg tttaacgatc gcgccatctc
    18301 ttatcgtgtg caccagggtt acgatcaccg tggtgtggcg ctctccgccg gtgttcaacg
    18361 gatggtgcgc tctgacctcg catcttctgg cgtgatgttc tccattgata ccgaatccgg
    18421 ctttgaccag gtggtgttta tcacttccgc atggggcctt ggtgagatgg tcgtgcaggg
    18481 tgcggttaac ccggatgagt tttacgtgca taaaccgaca ctggcggcga atcgcccggc
    18541 tatcgtgcgc cgcaccatgg ggtcgaaaaa aatccgcatg gtttacgcgc cgacccagga
    18601 gcatggcaag caggttaaaa tcgaagacgt accgcaggaa cagcgtgaca tcttctcgct
    18661 gaccaacgaa gaagtgcagg aactggcaaa acaggccgta caaattgaga aacactacgg
    18721 tcgcccgatg gatattgagt gggcgaaaga tggccacact ggtaaactgt tcattgtgca
    18781 ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg gagcgttata cgctgcattc
    18841 acagggtaag attatcgccg aaggccgtgc tatcggtcat cgcatcggtg cgggtccggt
    18901 gaaagtcatc catgatatca gcgaaatgaa ccgcatcgaa cctggtgacg tgctggtcac
    18961 tgacatgacc gacccggact gggaaccgat catgaagaaa gcatctgcca tcgtcaccaa
    19021 ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt gaactgggca ttccggcggt
    19081 agtgggctgt ggtgatgcaa cagaacggat gaaagacggt gagaacgtca ctgtttcttg
    19141 tgccgaaggt gataccggtt acgtctatgc ggagttgctg gaatttagcg tgaaaagctc
    19201 cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg atgaacgtcg gtaacccgga
    19261 ccgtgctttc gacttcgcct gcctgccgaa cgaaggcgtg ggccttgcac gtctggaatt
    19321 tatcatcaac cgtatgattg gcgtccaccc acgcgcactg cttgagtttg acgatcagga
    19381 accgcagttg caaaacgaaa tccgcgagat gatgaaaggt tttgattctc cgcgtgaatt
    19441 ttacgttggt cgtctgactg aagggatcgc gacgctgggt gccgcgtttt atccgaagcg
    19501 cgtcattgtc cgtctctctg attttaaatc gaacgaatat gccaacctgg tcggtggtga
    19561 gcgttatgag ccagatgaag agaacccgat gctcggcttc cgtggcgcgg gacgctatgt
    19621 ttccgacagc ttccgcgact gtttcgcgct ggagtgtgaa gcagtgaaac gtgtgcgcaa
    19681 cgacatgggg ctgaccaacg ttgagatcat gatcccgttc gtgcgtaccg tagatcaggc
    19741 gaaagcggtg gttgaggaac tggcgcgtca ggggctgaag cgtggtgaga acgggctgaa
    19801 aatcatcatg atgtgtgaaa tcccgtccaa cgccttgctg gccgagcagt tcctcgaata
    19861 tttcgacggc ttctcaattg gctcaaacga catgacgcag ctggcgctcg gtctggatcg
    19921 tgactccggc gtggtgtctg aactgttcga tgagcgcaac gatgcggtga aagcactgct
    19981 gtcgatggcg attcgtgccg cgaagaaaca gggcaaatat gtcgggattt gcggtcaggg
    20041 tccgtccgac cacgaagact ttgccgcatg gttgatggaa gaggggatcg atagcctgtc
    20101 tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg gctgaactga agaaataaaa
    20161 taaatccccg gcggcgtttt gtcgccgggt ttatgtgatc cccgaagatg aaacttattc
    20221 aatctcttca cagacatcct gcgttaatcg ccgcataata tctttccgta acaaaaactt
    20281 ttgtatttta cctgaggcag tgcgcggtag tttttcgatt accacgatat gttcaggata
    20341 tttatatttt gcgacccgtt tacggctaaa aaaagccacc acctcttcca gcgataatga
    20401 atgatgcggc gctttcagca cgacataagc gcatgatcgt tcacctaaac gttcatcggg
    20461 cattgcaacc acacaggcat cgtgaatttt aggatgctgc aataaaatat cttccacttc
    20521 acggctgcta atattttcgc cgccgcggac aataatatct tttttgcgtc cggtaatttt
    20581 tatatagcca gcctcatcca tacggcagag atcgccgctg taataccagc cttcttcatc
    20641 cagggcacgg gcggttaatt caggttcatc aaaatatccc ataaacacat tggggccacg
    20701 cgaggcttct tcaccttcgc aacctggcgg taaggtcttg cgtgcgtcat cgaccacttt
    20761 aatctctaca cctgcggcag cgtaaccatc ggtgtgcata aagcgcgaca aaggatcatc
    20821 gagattcacc accgcatgcg gcgaactttc tgtggaacca taaacactta ataatttaat
    20881 gccgcgctgc tggcattcac gcgccacttt tttggggatt gtggtaccgc cgcaaagaaa
    20941 gaagcgcagc gctgaaaggt ctgcgggttg tttctctagt aaattcaaaa gatcatagac
    21001 aaacggcgtt gcgccgagca tacaggagca acgctgctgc tcaagcagcg cgagacacgc
    21061 atcaggagtg aaaatatcta acaacacgct gcgagcgcca attaagaatg gtgccgttac
    21121 gccatgcaga aagcccgttg cgtgaccaag tggcgcaggc atcataaaga catcctgcca
    21181 ggttagattc agccgcgcgc aataagcccg ctcactggcg agaatattgt tatgcgtaag
    21241 catcacgccc tttggcagac cctcggttcc ggaggtaaac agcaccgcag ctaattcatc
    21301 gccgtgggtc gttatcgccg tggtcagtgg ggtattgtcg gcgataatct gacttaatga
    21361 gagggaagag gtggcgggag ccagtttgtc cacgccgaca atttgttgta gttgtggaag
    21421 ctgattttgc agcggcagga ttaaatctac cggacgcgtt tgtttaaaca acgtcggtgt
    21481 aaagaacatt tttgcctgac acttattaag cacccatacc agttctgctt cccgccagga
    21541 aggcaacagc ggtacggaaa ccgcaccgat tttcaggcag gcaagataga taacggtaaa
    21601 ttcacaccag cctggcagtt gaaatgctat gcgatcgcct gattcaatac ccttcgctaa
    21661 catccagttt gccagacagc tcgcggcgtg atcgagcgcg ctataggtgt acgatgctcc
    21721 atgattatcg accacggcaa ttttgtctgg catcgcacga gcggtctgct gccagtaatc
    21781 ggccagcgaa gcatcgcccc atagcccttg ctgacgatac gccgcacgac gttgttcgtt
    21841 aaacgttaat gtcactttca ttttggactc tcgaaacaga acatcaggcc cgagatgcgg
    21901 gcctgtggga tgcatgatta gccgtagcga aaatcaatac cgaaggtgcc tgcgggatat
    21961 tgccactgtt cgagaatagt gttaccgcac agcacccgac aggtgccgca ctccagacaa
    22021 ccggcggaat caaaatgaat gtttcctgcg tcatcctgct tataaagtcc ggcagggcag
    22081 gctttcatta atttacggaa ttcattgata tcgggatttg ccgccaaaat gatatgcggg
    22141 tggccctcat caacatggaa tttattgacg cctaatttga tgtcgacgtt aaccgtagcg
    22201 ttctggctca tagcgcggtt gctcccttaa tgccatcttt cagcaagttg atcagcccaa
    22261 ttttcttcgc gtgtcccatg atcattttgc gtaccggctg gttaggctta ccgtcaatgg
    22321 tgaacatctc gttcatgatg tcggctacca ttcgtgggta ttggctaaac aggcgcgggt
    22381 tttccatcag cgccgggatc ttgcgaaaat gctgcatatc acgcatgacg cagctttgtt
    22441 ccagctcacg tttgtattgc gccagactgc tggcggagaa atccgcgcgt tctttggcgg
    22501 cgatcactgt tgtggcggca gcctgagccg atgcaatggc taaatccatg ccgcggaccg
    22561 taaaacccaa attcaggcag aagcctgcgg cgtcaccaac gatcatcacg ccatcgttaa
    22621 ccagttgcgg taccatcgcc agaccgcctt ctggcaccat atgcgcggaa tattcaagca
    22681 gtttgccgcc gctaatcagc gggcgaatgg cggggtgttg tttaaaatct tccagcattt
    22741 gcggcacgct tttttgcgca tgggcgatat cagccaatcc acaaaccagc cccaaggata
    22801 tggaatcctt gttggtatag agaaatcccc cgcccatcag gccgtcagaa ggggaaccgg
    22861 caaacagcca ggcggcacct tcctcgcccg taatattaaa gcgatcgttg atctgttctg
    22921 gtgtgaggcc aataacctct ttaacaccaa cagcgtaatg atgcggatcg gaagcgggaa
    22981 ccattcccag cgagcggcca agcatcgagt taacgccatc ggctaaaatc accacattgg
    23041 cttcgagaat atcatccccg gcctggactc cagtgacctt gtttccttca cgaaccaacg
    23101 catcgacgcg aactcccggg ataaactgtg cgcccgcctg ctcggcttgt tccatcaacc
    23161 acgggtccag acgattacgc aatacggtat aagatgcgtg ttgcggaaca tctggttgct
    23221 cgcggtgaaa atcgagggta acggcgctct cttcggttaa gaaggagatt ttctcgcgtg
    23281 tgaccttgcg ttctaccggc gctgatgctg caaagcctgg aatgattgct tcaagtgtgt
    23341 gggcataaag acgcccgccg gtcatgtttt tacatccggc actgtcgccg cgttctatca
    23401 ccaggatatc cagcccggct cgcgccatga ccagtgcggc aacgctacca gcaacgcccg
    23461 caccgaccac aatggcatca aatttgtcat ccgacatgcg ttatgctccc ttcccgctgc
    23521 gtttacagcg ggagatgtgg ttgaaagggg aattagcggc tcaactggct aatcagggca
    23581 gggacgactt tgtagatatc gcccaccaga ccgtagtcgg catagttgaa gattggcgca
    23641 tttttatctt tattgatggc gacaatcact tttgcgccgt tgccgccaac catatgctgg
    23701 atctgcccgg agatccctag cgtcaggtag agatcggatt tcagcaacac gccggagaca
    23761 ccgatataac gttcacgctc catccagttc tcgccttcgg caattggacg tgaacagccg
    23821 acttcagcat tcagcaccgc cgccagttcg tggaccattt tcaggtcatc ctgcgctgcc
    23881 agaccacgac cgacgccaac cacacgtttt gctttgctca ggtccacgct gcttgcggct
    23941 ttcgcacggc gttcgcgaca gagaatttca tgacgcggag caacgtaagg taccgtttct
    24001 gtcgggcact gatgagaggt atcactggtg cacggttcct gaacaccggg tgcaagggta
    24061 atgatcgcca gcgagctgtt gatcttttcc tgagcgaacg ctaacccgcc atacatccgg
    24121 tgttcggcgc aaatgtgacc atcgacaata tccaccgccg tggcatcgtt caccagcgcc
    24181 gcattcagtt gcacacttaa ccgtgctgcc agcgctttac cacgtttcgt cgcggccagt
    24241 agcaacatag cggggtgttt atctttcagc agagcagcaa tgctttcggc gtaattttca
    24301 gtgcgttgca gcgcgtcgtt ttgctcaaga acataaatac attttggacc ataaggcata
    24361 actgcctgcg cctggtcggt attttgtaca atggcataca cctgttggcc ccattgctga
    24421 gcgccgccaa acagttcagc ataacgttca ggattatcgc taaagaccca gacgctgttt
    24481 aattgactca tcgtttcttc tccgtaggct tcagttcagg gctttcttca gatgttccgc
    24541 cagctcggca atggcctccg gcgaatcgtt atcgagaatg atgtgcttac gttctgtttg
    24601 cggcggtacg cgaatgccgg tcagttcagc aagtggtgcg ctctggctcc agccaatatc
    24661 acttgcctgc cactgattta ccggtttttt acccgcgccg agaatggctt tcatcgaagg
    24721 aatgcgtggc acgttaatat cggaggtgac gcagagcacg gctggaacag agagttcaat
    24781 aacttcaacg tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct gacgctgaat
    24841 agcactcact gcattaatca ccggaagttg cagaatttct ccgaccagca agccaacctg
    24901 ctgggcataa aggtcgccgg aacctttacc aaagatcagt aaatcgaagc cgatcttttc
    24961 aactgctgcc gccagcgctt ttgcggtatc gagaggcagt gcatgctcaa gttgtgcatc
    25021 ctgcaccata tacaggctgt gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt
    25081 ctgcaacaat gagccgccaa cggtcagcgc ggctatctca tcgtcatctg ttgcaagctg
    25141 gcttgcagct tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa
    25201 attcagggtg tattctggag tgacaacaat gtcctgttct tcaggcacca gcttaaagca
    25261 ggttattatt ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt
    25321 aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga gcaagctgtt
    25381 ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg caatttgcgg
    25441 caatatagtt attccatgac cgtacaatct tgcattcagt gtaaataatc ttgtttagct
    25501 ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc atatattgtt
    25561 tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga taatgccagt
    25621 gaaacgctgt ttgtcgctgg taaaacgcca cggctttcac gttttgcatt tagcgatgat
    25681 ccaaaatggg agtctggaca tcacgttcat gacaatgaaa ccgagctgat ttacgtcaag
    25741 aaaggggtgg caaggtttac catcgattct tcgttatatg tcgcgcatgc agatgatata
    25801 gtggtgatag aacgcggcag gctgcatgcg gtggcctctg acgttaacga tccggcaacg
    25861 acgtgtactt gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa tcatctactg
    25921 caaccgcatt cttgtccggt aattgccgcg gggcagggaa aagaagtcat taaaacctta
    25981 tttaatgagc taagtgtgat tttgccgcaa agtaaaaata gccaaacatc ttcgttatgg
    26041 gatgcatttg cctatacatt agcaattctt tactacgaaa actttaaaaa tgcttatcgt
    26101 tcggagcagg ggtatcttaa aaaagatgtt ctgataaaag atattctttt ctatctgaat
    26161 aataattatc gcgaaaaaat cactttagaa cagttatcga aaaaatttcg tgccagcgtc
    26221 agttatattt gccatgaatt taccaaagag tatcgtattt cccctattaa ctatgttatt
    26281 caacggcgta tgacggaagc aaaatggtca ctcactaata ctgaattatc acaggcagag
    26341 atctcctggc gtgtgggtta tgaaaatgtc gatcactttg ccaaactgtt tttgcgccat
    26401 gtcggctgtt cgcccagcga ttaccgcagg caatttaaaa actgttttgc ggaacaagaa
    26461 atcctatctg aatttcctca accggtaagt cttgccggat aaatctggcg gcgcagtttg
    26521 ttaagaagct gcgccgcctg cagattattt gttctgatag tctttcagga tctgccgacc
    26581 tgctacgtaa atcataattt cgtctgtacc gccgccaata cgttcacaac ggacatcacg
    26641 ccagaagcgg gagacgcgcg cctcatcggt atagcccaga ccgcccatga tttgaatcgc
    26701 atcatcaatg acttccattg cggtacgtgc acagtacagt tttgccagcg ccgcgctggt
    26761 gcgcagtgat tgatgctgat cggcttgcca tgccactttc agcaccatgt tgcgcatgtt
    26821 gtcaatctta atcgccatca gcgccagttt ttcctggatc atctggttat gaccaatggg
    26881 tttaccaaaa gcgatacgct ggttggcata gcgagcggca tcttcaaagg cacactcggc
    26941 aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc tcaaagttgt acatcacatt
    27001 gaggaaaccc attccttctt cgcccaccat gtcgctctct tcaacttcca cgttgtcgag
    27061 atagacttcg caggtgctga gcatatgcca gccgatttta tgcagcgggt taatcttaat
    27121 gccgggctta ctggagtcga cccaccacag ggtgaaggct tttttgggat ctttcggttg
    27181 cggatcgcgc gccagcacca gcatatacgg gtactctttc gcaccggtaa taaaggtttt
    27241 ctgtccgttg atgtaaacct tgccgttttt acgcgtgtaa gtggtagtgg cactgttgtt
    27301 atctgagcca gcacctggtt ccgtcaacgc cagggcatag gcgggatcac ccgtttccag
    27361 ggtgctttct gccgttttac gtagctgctc tgcagaaccg aaacggcgca tactgtgaat
    27421 acattgaccg ttggtaatta aaaatgccgg agcaccgcat tttgacactt ccatcagcgc
    27481 cagcatttgg gtgacgtaat cagcagggat accaccaaat tcttccggca cgccaagcat
    27541 ggaaataccg ttatccgcca gtgcccgcat aaactcacgc ggatatgtcc cgttttgatc
    27601 gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc agttcgcgaa tactggccag
    27661 cagcagttct tgttcttcag ttaaagaaaa atccatcgtg atactcctgt taatgagccg
    27721 cttcaggcag gacaaaaccc atcgccgcat cgataaataa tctttcgtcc atcagtttga
    27781 gttctggcga aatcactggg gtgaaatcca ttttgtcgag aatatctttt tgtaaatcga
    27841 cgccaggggc gatttcgatt aaatgcagtc cgtcttcttt cagcgtgaat acggcgcgct
    27901 cagtgatata acgaacatcc agccctcgct cgagagcgat ttttccgcta aaagtaattt
    27961 ccggtagttc ccgaataaat ttcttcaccc gtccttcctg gacgatattc aatttgccat
    28021 cggcaatttc tgtttttaaa ctgcccgcag ttaacgtgcc gcagaaaata attttcttcg
    28081 aggtggcact gatatcaata aatccaccgg tacccatgat tttgccattg aatttatgca
    28141 cgccgacgtt accgtgctgg tcgacttcag caaaactcaa ataacaaaca tccagaccac
    28201 caccgtgata aaaatcaaac tgggacgtca tatccagaat ggcacgggta ttcacgttcg
    28261 cgccaaaggc gataccctgt gaagtaattc caccaatcgg acctgtttct accgtcagaa
    28321 taaagtcatc agcacaacct tcttcccggg cgaccaggcc aatgccgtca gcaataccga
    28381 cgccgacatt ccccaccgcg cctttacgca tttcgaataa tgcacgccgt gcaactaatt
    28441 tacgttggtt taggggcagg ctaagtttgg tgctgtcatc aagggtgaag tcaccagaaa
    28501 taaagcggtt aaccggcgca ccgccatata gttggctttg atccggatcg accaccacaa
    28561 tatccaccag ataacccgga atacggacag atttaggatg cagtgtggct ttcttaacca
    28621 ttttctgcac ctgcatcatc acaataccgc cgttattgtg caccgcctgg gcaataacca
    28681 atgcgtcgag atacatcacc tcatcttcaa aagtggcgta gccttcactg tcgcaggtgg
    28741 tagcgcgaat gaaagcaata tctggcgcaa tcgctttgta atagagatat tctttgttat
    28801 caaactcgac cagtttaatc aggtcttctt tagtgacttc attcagtttg ccgccttgct
    28861 ggcgtggatc gacaaatgtc ccgatgccaa tatcactaat aataccaggc tggtgggctg
    28921 cggcggcgcg taaggtttgt gtaagtacac cttgtggata gttataagca ataattttat
    28981 tttgttctgc gagatcagaa atacgcggcg attgtcccca gtgaccgcat aatgcccatt
    29041 tcaccagacc ttcttgcgcc agcggactaa taccgcggtc ggcgcgatcg ccaagccctg
    29101 ttggactaat aatcgataaa ttacgtggcg tttgagtctg tttatattta tcagcaagag
    29161 cagtaattaa cgtggtggct tccagaatac cgccgccagc gcctaacaca caaagtgttg
    29221 cttcgtcggg aatataattc accgcttcct gtgccgacag gaccggcact cgtccattaa
    29281 tacgaggtgg ttttacaggt ttcattgtta attcctgatt caggtgtgat tttccctacg
    29341 ccagatggat aattagcgca cagagactca cgaaatattt ttattaaata cggttgaata
    29401 tattttttta gttcggcggg ggaggatatt cccgccgaaa tattattgct tatgcctgat
    29461 gtaaaatagt taatactgtg cgcaaatcat ttaccgagat ttgccctggc gcagaggctt
    29521 tttttaccgc accaaaagtt gccgccgagc caaatacttc accagccaga cgagaaatta
    29581 cgccagtttt tgccattgac atcgtgatga ttggacgatc ggcatactgc tcctgcatct
    29641 ccagggtcgc ggcaagcaac gccagcacat cgctggtact ttgcggcatc agcgcaatct
    29701 taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt tcttcggctt
    29761 ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc gcgtgggcgt
    29821 aggcgacggt ttctttaacc tgatcatcgc cggtaaataa ctccagatcg atcatatcaa
    29881 ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg gaaatcgcct
    29941 gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct ggcatggttt
    30001 cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg gcaaagtggt
    30061 ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct tcggatttca
    30121 ctcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc gtaccaatga
    30181 cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg tcagcctgtc
    30241 aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg ccagtccata
    30301 acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc gttttgcaac
    30361 cagcttgttg cgcctgctgc aataacttcg tcatatgcgg gttatacacg cattcagtga
    30421 ccagcagtcc cggatgtaac agactgatat cattaaccaa tgatttattc tcaaggggtt
    30481 tcatacccac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct tcagcaaagg
    30541 cttgctgatc ggcgagatcg gtaaccgtga cgacacaatc ggtgttttcg ttaacccgct
    30601 gcgcgaaggc gagggcttta tcgaagaact tatcccgacg gttaaagagt ttaatttctt
    30661 ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg gcccctaaca
    30721 gcaccattgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga atatggcccg
    30781 tgccgtcggt gttatagccg cgcaggtagc catcatcatt aacgatggtg ttgatggcac
    30841 ccaccagttt ggctgctggc gttaattcat caacatattc acacgccagt tgtttgttcg
    30901 gcattgatac accagttccg cgcattttta gagcttttaa tccttcaatt gctccgggaa
    30961 agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct ttttctaagg
    31021 ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc aacccaatca
    31081 attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct taattgtgtt
    31141 taaccgggtg ctaaatttgc gctcgccaaa ccgcacatca ttctcaggaa taataaaaac
    31201 gcggtaatag cggataaaaa caataattgc ggtaatcagg gccagcagcg cgaaagtaaa
    31261 atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa tcagcggaat
    31321 aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg ctttgctttt
    31381 tgggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag ctgctgagaa
    31441 accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt agataatggc
    31501 tgctgttatt gttgccagtg cagagttaaa tacattagcc cagatgggcc ggaccatttt
    31561 tttcagtagt gcggcaaaaa taaagacaca gaccagcgag cccatactgt aataagagat
    31621 ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt tgggcatcca
    31681 caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg atgaaacacc
    31741 ttccagccag actaacggtt tgctgttcat ttgcggtaat tcattagcta cgctggcgtc
    31801 caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta caaacagaat
    31861 acccggaata atcagcccat agccgtacca gatattattg agcaacatat agctcaccag
    31921 cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga ccgccgaacc
    31981 agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca gcgctgagtt
    32041 agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc aagcgggaat
    32101 accaaagaag aatagcagat acattattac tgccattaat atcaccgccc gacgaccaaa
    32161 cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac cgataccgga
    32221 aattaagtag gcaatgcccg cgttgtcagt ggaaaacttt tccgccagag atgacatatt
    32281 ttgggcaagc gtaataacac taatgccgtg caggaagtag ctgaagtaaa tacaaagaac
    32341 agccaggata aatggcgtgc tgaaagcctt attttgagac attttcacag ctccttgctg
    32401 aaagataatt tcgttaccgt acgtattgtt ctccgacggt ttacataatt actatactga
    32461 agatgatttt ccctgggtac atggcataaa gtttatgttt ccaggaatat tgcatgtcat
    32521 agtttgtttt tgatcgcgat tttccttagt tagcgcagaa agagttattt atccgtaaga
    32581 tcttgcatga cctgaaaaga atattgcatt acccacccgg agcgacattt tcctttagcg
    32641 agtgatgccg cgtttttttg cggctgcgta agccgataaa cagtgccagt aaaaaaccga
    32701 tggcagcgat ggcggtatcg aaccacataa tatcggcaat acttctttgg gacagatgag
    32761 ccgtaatcaa cggaatagta aaggtcgcaa tactgcccgc actgtaatag atccctgtag
    32821 ctttaccttt agcgtaaggg aaacgttcag ccattaacgt caggccaatt tgcacaacac
    32881 ctccagcaga agtaaaacca atgacaaaag aaaatattat caccacataa aatgtgggat
    32941 gcaggcagac ggtaaacaga gcaataaatg agataaaggt gtacagcatc agtaatgttg
    33001 ttgggcgaac ggtattacga atgagtggag cggtaataaa tacacaaagc agcgaaccca
    33061 cggtgtagat actgagtagt ttgatcgaca tagtgtatga catgcctgca acaaattgtc
    33121 cgtactgtgc cagccactgg ctaaccagat aaaacgttgc cattgagata tagccatata
    33181 aggtataact ggctaaatcg ataattgaac agcgatgttc cgtagagctg gtggttttct
    33241 ttatgacagg taagcgacga cccggatggg gtgggaacgt acaacggtat aaaaacagag
    33301 cgttaataaa cataatgcct gcagcaatca taaaggacca accgaaccac agttcagccc
    33361 acactaacag gctaatgatt agcggtaata aaaattgtcc gctggaaaca aatgctttaa
    33421 ttaaaatatt ggctgtccca ggtgagcgtg gaaaagcttc catcaaactg ggataagtgc
    33481 ctgcatcgag aaaactgttt gccattcccg ccagaaagcc aaaaacataa gcgataatga
    33541 tgttattggt ctgcaggatg ccaaaaaaga aggccatata gcagcacatc ccgagcatga
    33601 taaaagggcg gcgaccaaag cgatcggata ataatcctgc aaaaagcaag acacttaatc
    33661 gaccaatgcc cagcgatgag ataactatcg agacacccgc ggcattagac tgccaaagtg
    33721 tctccagcga ggccatattc aggctcatca aaatgacgcc cataccatgc accaggtaat
    33781 taaaatacaa cccaagtgcg gtagggtaat agggattttt cataggttct acttagcaaa
    33841 ggtgctttaa aaagtgtagc gatgttgtgt cagtatccgt tcattattgt tgtaacaacg
    33901 acaattcctt tttgtggaga aattaacaaa gccgctccag atcgtgtgca tataattcag
    33961 cggctaccga ttgatagatc ttccagtcaa taaaattgcc atcagggtag acttgttgaa
    34021 acgcggttaa gtcggggaaa aaacgcatgg tgttgttgcc gatgcggtta tttattttct
    34081 ctatgatcgc atgaagatgt ttttttcttt gctgacgcat agccaacagt tgctcgacta
    34141 cacaatcagg aatggcacac ctgccatttt cccattgacg ccagctttca ctgtcaccgg
    34201 tctgggcaat ccaggtcgcg cattcatcaa tagtcatggc aaaaatatgg cggagtgcct
    34261 ggagttcata agcgttcatc gtgtaatggc tgcatgaaat aaacaataaa atataaataa
    34321 cattatcagc ctgctgacgg cttgaagaga gtcacagtat cttgtgcaac attattggaa
    34381 agagtgaggg gcgaggtagc gaagcggaaa aatgttaaaa aaaagcccat cgtgggagat
    34441 gggcaaagac tacacacagc aattcgttgt ttcactcagg ggatttccat gcttataaat
    34501 caatatgttg atttataacc gtgagctaat agtaggcata cggacttttt gcgtcgatca
    34561 gattcgtctc attagtttaa aaactgtaaa gaatttgcga caggtactac cagctgaatg
    34621 gcggtaagca ggaaaatatg gggaagagca agcaaatatc aattagcggt ggttaacaat
    34681 caggcggcag agccgcctga tgagaaatta cttattcggt ttctggattt cgccaagttc
    34741 ttcgagaatt tcttccggtt gatccgtcgg cggtgggacc tcttccaccc acgcggcaaa
    34801 cagacgccag gaaacggcta acagaaccgg accaataaac agaccgatca tcccgaaagc
    34861 aatcaaacca ccaataacgc cagagaggat caggatcagc ggtaaatcgg cacccatgcg
    34921 aattaacatt gggcggatga cgttatccag cgtgccaacc acaccgctcc acactaacaa
    34981 taccgttccc caggtggtat cgccagtcca gtagagccag ataatcgccg gaatcagtac
    35041 cggcaacggg ccaagctgga caaggcagga aaggatcatt aacaccgtta gcaaagttgc
    35101 ataaggtacg ccggatacgg cgaggccgat accgccaagc actgcctgta ctaacgccgt
    35161 caccaccaca cccagcgcca ccgcgcggat agcctgtgcc gccagcagta cggcggcatc
    35221 tccgcgaacg cctgccagac gggtcgcaaa atggcgaatg ccttgtgcca cctgttcacc
    35281 gcgccagtac agcagggcac tgaagagaag catcagcgca caatgcacca taaagcgccc
    35341 gatatgcgcc gcttgcccaa cgaaccaggt agtggtggtg ccaatataag ggcggacttt
    35401 cgccatgatc gccgtccccc ccatatccag caagttgtgc cagcctgcat acagcttcgc
    35461 gccaatcacc ggaatggtat taagccacgc cagatcgggt aacgtcatgt caccgctgga
    35521 aatggcttta attagcgggc cgctgccgtc gacgatactg ttaaccagca aagcaatagg
    35581 gatgataaac accatcacta ataacagcgt catcaccaga acggcgagag agcggcggcc
    35641 aaacatgatc ttttgcaaac gtaacaatac cggccaggtg gcgataacca ccgtaccggc
    35701 ccatgcaaag ccgagaataa agggttgaac aatccacaga catgccacaa tcatgatggc
    35761 taaaaacagc accgaaagca gaatttgtgc gacatccctg ggctgacgaa catttaccat
    35821 aactactttt cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc
    35881 acctgcataa tgatgagcaa tttcagcggt ttttaacagg ccgattctgc ccttaattct
    35941 gatgggcgtg ttagaaaaaa atgtggtaca acaactcagg caacacgcaa acgatttact
    36001 cgtcgtattt caagttgcat atgctgcggc tacgttcgtt caccccagtt acttactgtt
    36061 gtaagctcct ggagttcact cgcttgccgc cttcctgcaa ctcgaaatcc atagagtaaa
    36121 taaacatcac ctttctgtgc gctattgcag ccgcctgaaa gaacagtgta tcgacctaca
    36181 tcacagaccg caggaaaggg tcaatataat gattccacag atttcccagg cacccggcgt
    36241 cgttcaactg gtgcttaatt ttttgcaaga gctggagcaa caaggtttta ccggcgatac
    36301 ggcgacaagt tatgctgatc gtctgacaat gtcgaccgac aacagtattt accaacttct
    36361 ccccgatgca gtggtatttc cgcgttcaac cgcagatgtg gcgctgatcg cccgtcttgc
    36421 cgcgcaggaa cgctattcat cgctgatctt taccccccgc ggcggcggca ccggcactaa
    36481 cggtcaggcg ctcaaccagg gtattattgt tgatatgtcc cgccatatga accgcatcat
    36541 cgaaattaac cctgaagagg gctgggtgcg cgttgaggcc ggggtgataa aagaccaact
    36601 caatcagtac ctgaaaccgt tcggctactt ttttgcacca gaactttcga ccagcaaccg
    36661 ggcaacgctc ggcgggatga tcaataccga tgcatccggt cagggatcgc tggtctatgg
    36721 caaaacgtca gatcacgtac ttggcctacg tgcggtattg ttggggggcg atattctcga
    36781 tacgcaacct ttacccgtcg agctggcgga aacgctgggt aaatccaata ccacaatcgg
    36841 gcgaatttat aacacggttt atcaacgttg ccgtcagcaa cgccagttaa tcatcgacaa
    36901 cttccccaaa cttaaccgct ttcttaccgg ttacgatctg cgtcatgtct ttaacgatga
    36961 gatgaccgag ttcgatctga cgcgcattct gacgggttca gaagggacgc tggcctttat
    37021 taccgaagcg cggctggata ttacgcgctt gcctaaagtg cgccgtctgg tgaatgtcaa
    37081 atatgactct tttgactctg cgctgcgtaa cgcgccgttt atggttgagg cgcgggcgct
    37141 ttcggtagag acggtggact caaaagtgct gaatctggcg cgggaagata ttgtctggca
    37201 ttccgtcagc gagttgatta ccgatgtgcc tgacaaagag atgctcgggc tgaacattgt
    37261 ggaatttgct ggtgatgatg aggcgctgat tgacgagcgg gtaaatgcac tctgtgcgcg
    37321 gttggatgag ctgatcgcca gtcatcaagc aggtgtcatc ggctggcaga tgtgccgtga
    37381 gctggcgggg gttgaacgta tctatgcaat gcgcaaaaaa gccgttggtc tgcttggcaa
    37441 tgccaaaggt gccgctaagc caattccgtt tgctgaggat acctgcgtac cgccggaaca
    37501 tctggccgat tatattgctg aatttcgcgc gctgctcgac agccacggct taagctacgg
    37561 tatgttcggt cacgtcgacg caggtgtctt gcatgtccgt ccggcactgg atatgtgcga
    37621 tccgcaacaa gagattttga tgaagcaaat ctctgatgac gtggtggcgc tgactgcgaa
    37681 atacggtggt ttgttgtggg gcgagcacgg caaaggtttt cgcgctgaat acagcccggc
    37741 gtttttcggt gaggaacttt ttgcagaact gcgcaaagtg aaagcggcat ttgacccgca
    37801 taaccgactc aacccaggga agatttgccc gccagaaggt ctcgatgcgc cgatgatgaa
    37861 agtggacgcg gtgaagcgcg gtacattcga tcggcagatc cccattgcgg tacgccagca
    37921 gtggcgcggt gcgatggagt gtaacggcaa cggtttatgc ttcaactttg atgcccgtag
    37981 tccgatgtgt ccgtcgatga agatcaccca gaaccggatt cattcaccga aagggcgcgc
    38041 aacgctggtg cgtgaatggc tgcgtttgtt ggcggatcgc ggcgttgatc cactcaaact
    38101 ggaacaagaa ctgcctgaat ccggcgtcag cttgcggacg ttaattgccc gcacgcgcaa
    38161 tagctggcat gcgaataaag gcgaatatga cttctcacac gaagtcaaag aggcgatgtc
    38221 gggctgtctg gcctgtaaag cttgttcgac ccagtgcccc atcaaaattg atgtgccgga
    38281 gtttcgctct cgttttctgc agctctatca cacccgttat ttacgcccgc tgcgcgacca
    38341 cctcgtcgct acggtcgaga gctacgcgcc gctgatggca cgcgcgccga aaacctttaa
    38401 cttcttcatt aaccagccgc tggtgcgcaa actctcggaa aaacatatcg gcatggttga
    38461 tttgccgctg ctgtcggtcc cctcgctaca acaacaaatg gtggggcatc gctcggcaaa
    38521 catgacgctg gaacagcttg aatctctcaa tgcagagcag aaagcgcgca cagtgttggt
    38581 ggtgcaggac ccctttacca gctattacga tgcgcaagtg gtggcggatt ttgtccgtct
    38641 ggtcgaaaaa ttaggtttcc agcctgtgtt actgccattt tcgccaaatg gcaaagccca
    38701 gcatattaaa ggttttctta atcgttttgc gaagacggcg aaaaagacgg cggatttcct
    38761 caaccgtatg gcgaagctgg gtatgccaat ggtgggcgtc gatccggcgc tggtactttg
    38821 ttatcgcgat gaatataaac tggcgctggg cgaggagcgt ggcgagttta acgtcttact
    38881 ggcgaatgaa tggctggcaa gcgcacttga gtcacagcca gtggctacag tcagcggtga
    38941 atcatggtat ttctttggtc actgtaccga agttaccgcc ttgccgggtg cgccagcaca
    39001 atgggccgcg atatttgccc gttttggcgc gaaactggaa aatgtcagcg tgggttgctg
    39061 cggcatggca gggacttacg gacatgaagc gaaaaaccat aaaaattcgc tcgggatcta
    39121 tgagttatcc tggcatcagg ctatgcagcg actgccgcgt aaccgctgtc tggcgaccgg
    39181 atattcctgc cgtagtcagg tcaaacgggt tgaaggcacg ggggtacgcc atcctgtgca
    39241 ggctttactg gagattatta aatgatatgg aaacgaaaaa tccccctgga agcactgaat
    39301 gccatgggtg aaggaaacat ggtggggtta ctggatattc gctttgaaca tattggtgat
    39361 gacacccttg aagcgacaat gccagtagac tcgcggacaa agcagccttt cgggttgcta
    39421 catggaggtg catccgtggt actggccgaa agtatcggtt ccgttgccgg ttatttatgt
    39481 accgaaggtg agcaaaaagt ggttggtctg gaaatcaatg ctaaccacgt ccgctcggca
    39541 cgagaagggc gggtgcgcgg cgtatgcaaa ccgttgcatc tcggttcgcg tcaccaggtc
    39601 tggcagattg aaatctccga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc
    39661 gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg
    39721 cacagtaaac ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact
    39781 caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca
    39841 cctgcccaat atcttaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact
    39901 ctctcttctg ctgaattaaa agaagaaatc tactttgcct ggcgtctggg cgtgcattga
    39961 tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga
    40021 tgaccttctt aatactgctt atctacccgc ctgatattgt tcaaaaataa tactaatcgc
    40081 agtttgtgtt aaaacggcgg cttagcttta tgagattatt cttatcgccc cttcaagagc
    40141 taagccactg agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt
    40201 cgcacagact taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa
    40261 tggtcatttt caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata
    40321 atcattatct aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg
    40381 tctatgctta ttaaaagcag gcaaaaagca tgttttacgg taaagcccct gcctttgctg
    40441 ggttgaactg ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag
    40501 gtaaatcgat ggacatgcat tcaggaacct ttaatccaca ggatttcgcc tggcaaggct
    40561 taacgctgac acccgcagcc gctgtccaca tccgcgagct ggtggcaaag cagccgggta
    40621 tggtcggcgt gcgcttaggt gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg
    40681 acagtgttag cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt
    40741 ttgtcccgct gcaagcgatg ccgtttattg atggcacgga agtcgatttc gttcgtgaag
    40801 gacttaatca gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg
    40861 aaagctttgg ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa
    40921 aacctggacc ggcggcccgc tgaattataa agaaggattc ttcacccagt tagccaccga
    40981 tgagctggca aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga
    41041 gccggagtgg atgctggagt ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga
    41101 accgcactgg ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc
    41161 ggcaccatcg tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca
    41221 aactggcgcg aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggtgt
    41281 tcccgtgcgg gaaggcaaag aggtggcggt ggatgccatt ttcgactcag tttcggttgc
    41341 cactacttat cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc
    41401 gatccacgat cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga
    41461 caacttcttt gccgcgctta atgcggcggt agcctctgac ggtacgttta tttatgtgcc
    41521 taaaggcgtg cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac
    41581 cggacagttt gagcgcacca ttctggtggc cgacgaagac agctacgtca gctacattga
    41641 aggctgttcc gctccggtgc gtgacagcta tcagttgcac gcggcagtgg tggaagtcat
    41701 catccataaa aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa
    41761 caacaccggc ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag
    41821 caaaatgtca tggacgcaat cagaaaccgg gtcagcgatt acgtggaaat atcccagctg
    41881 cattttgcgc ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca
    41941 tcagcaagcg gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgaccat
    42001 tatctcgaaa gggatctctg ccggacatag ccagaacagt tatcgcggct tagtgaaaat
    42061 catgccgacg gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc
    42121 taattgtggg gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga
    42181 gcacgaggca acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg
    42241 gatcagcgaa gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc
    42301 agaactgccg ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca
    42361 cagcgtcgga taaggaataa acatgttaag tattaaagat ttacacgtca gcgtggaaga
    42421 taaagctatc ctgcgtggat taagcctcga cgttcgtccc ggcgaagttc acgccattat
    42481 ggggccaaac ggttcgggca aaagtacctt atcggcaacg cttgccgggc gagaagatta
    42541 tgaagtgacg ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga
    42601 agatcgtgca ggcgaaggca tctttatggc cttccagtat ccggtggaga ttccaggtgt
    42661 cagtaaccag tttttcctgc aaacggcact taatgcggtg cgcagctatc gcggccagga
    42721 aacgctcgac cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat
    42781 gccggaagat ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg
    42841 caacgatatt ttgcaaatgg cggtgctgga accggagtta tgcattcttg atgaatcgga
    42901 ctccgggctg gatatcgacg cattaaaagt ggtcgccgat ggcgtgaact cgctgcgtga
    42961 tggcaagcgc tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc
    43021 tgattacgtt catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt
    43081 caaacaactg gaggagcagg gttatggctg gcttaccgaa cagcagtaac gcgctgcaac
    43141 agtggcatca cttgtttgaa gctgaaggga caaaacgctc cccgcaagca cagcagcatt
    43201 tacaacaatt gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc
    43261 cgctggaagg gctgaccaat agtcagtttg tcagcattgc gggagagata tccccacagc
    43321 agcgagatgc cttagcgtta acgttagacg ctgtgcggct ggtatttgtc gatggacgtt
    43381 acgtgcctgc gctgagcgat gcgactgaag gcagcgggta tgaagtgagc attaacgacg
    43441 accgtcaggg gttacccgat gctattcagg cggaagtgtt tctgcatttg acggaaagcc
    43501 tggcacaaag cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc
    43561 tgttaatgca tatcacccag ggcgtggcag gtgaagaggt gaacactgcc cattaccgac
    43621 atcatctgga gctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctta
    43681 atgatgctcg tcattttacc ggggcacggt tctctatcaa cgtcgcagcg aatgcccact
    43741 tgcagcatat caagctggcg tttgaaaacc cgctcagtca ccactttgcc cataacgatt
    43801 tgttgctggc tgacgatgcc accgcattta gccacagttt cctgctgggt ggcgcagtgt
    43861 tacgacacaa caccagtacg caactcaatg gcgaaaactg cacgctgcgg atcaatagcc
    43921 tggcgatgcc ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag
    43981 gtttttgtaa cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat
    44041 ttaacggttt gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca
    44101 ataatctgct gatgggcaaa ctggcggaag tggatacgaa accgcagctg gaaatctatg
    44161 cagatgatgt gaaatgcagc cacggcgcga cggtggggcg tattgatgat gaacagatgt
    44221 tctatctgcg ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct
    44281 ttgctgccga actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctgaccc
    44341 gaatcggtca acggctgcca ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc
    44401 cgactttccg gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc
    44461 cgccagtgcg cagaaaccga gccaggtgat tgacgccgag gccgagtttt atcgtcatgg
    44521 ctacgcggcg gtgcatcgcg gtattcatac cttaagcgcc caggcgaccg agaaaatgga
    44581 gaacgtgcgc aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt
    44641 cgtccgcggc acgacggaag ggatcaatct ggtcgccaat agctggggca acagcaacgt
    44701 gcgggcgggc gataacatca tcataagtca gatggagcac cacgctaaca ttgttccctg
    44761 gcagatgctt tgcgcacgcg ttggcgcaga gctgcgtgtg atcccgctca atcccgacgg
    44821 tacgttgcaa ctggagacgc tgcctacgct gtttgatgag aaaactcgcc tgctggcaat
    44881 tactcatgtc tccaacgtgc ttggcacaga aaatccactg gcggaaatga tcacgcttgc
    44941 gcaccagcat ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt
    45001 ggatgttcag gcgctggatt gcgattttta cgtgttttcc gggcataaac tgtatggccc
    45061 caccgggatt ggcattcttt atgtcaaaga agccttgcta caggagatgc cgccgtggga
    45121 agggggtggt tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc
    45181 accgtggcgg tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc
    45241 gctggagtat gtttcggcgc aggggcttaa taacatagcc gagtatgaac agaatctgat
    45301 gcattacgcg ctatcacagc tggaatctgt accggatctc actctctatg gcccacaaaa
    45361 caggcttggc gttattgctt ttaatctcgg taaacaccac gcctatgatg ttggcagttt
    45421 tctcgataat tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc
    45481 ctattataac gtccctgcta tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga
    45541 agtggatcgt ctggtgaccg gactgcaacg tattcaccgt ctgctgggat aacagggagg
    45601 cactatggct ttattgccgg ataaagaaaa gttgctgcgt aattttttac gctgcgccaa
    45661 ctgggaagag aaatatctct acattattga gctgggccag cgtctgccag aattacgcga
    45721 cgaagacaga agcccacaaa atagcattca gggctgccag agtcaggtgt ggattgtcat
    45781 gcgtcagaat gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa
    45841 agggcttatt gcggtcgtct ttattctcta cgatcagatg acgccgcagg atgttgtcaa
    45901 tttcgatgtg cgtccgtggt ttgaaaaaat ggcactcacc caacatctca ccccatctcg
    45961 ttcacaaggt ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta
    46021 aactaaaggg acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact
    46081 gacctgcaca ggagtgtttc ggtttcaggt gaacataagc gccatccgac gtttcagcgt
    46141 gagtctccgg caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc
    46201 ttatcggcgc ttttagtgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa
    46261 ccggaagccg actggttggg caaaatcaaa cgtatacggt gcaggaaggg gataaaaacc
    46321 ttcaggccat cgcccgacgt tttgatactg cggcaatgtt gatccttgaa gccaataaca
    46381 ctatcgcccc ggtgccaaaa cccggtacga cgataactat tccttcgcaa ctgttattac
    46441 ctgatgcgcc gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc
    46501 cgccgggaga aaatatcgtg caggtttatc cgataggtat tggcttgcag gggctggaaa
    46561 cgccagtgat ggaaacgcgt gtggggcaga aaatccctaa cccaacctgg acgcctacgg
    46621 caggcattcg tcaacgttcg ctggagcgtg gcattaaatt accgccagtc gttcctgccg
    46681 gaccaaataa cccgctagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc
    46741 tcattcatgg caccagtgcg ccggacagcg tcggtttgcg cgtcagttca gggtgtattc
    46801 gcatgaatgc accggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga
    46861 aagtgatcaa cgaaccagtg aaatattccg tggagcctaa cgggatgcgt tatgttgaag
    46921 tacatcgacc actatcggca gaagaacagc agaacgttca gacaatgcca tacacactgc
    46981 cagcaggctt tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag
    47041 cgttgtatcg tcgggcaggg tatccggttg cggtgagcag tggagcaact cccacagcca
    47101 gcaatgcgcc ttcagtagag tcagcgcaga atggtgaacc agagcaaggg aatatgttac
    47161 gcgcgacgca gtagtggtaa atagcaaaca aaaaaatggc gcacaatgtg cgccattttt
    47221 cacttcacag gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag
    47281 cacgagctgc gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc
    47341 tcagctggtc aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag
    47401 cgttgctgga gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt
    47461 tagtagcttt cattattaat accctctaga ttgagttaat ct
//
LOCUS       DAZGXS010000022        44258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGXS010000022 DAZGXS010000000
VERSION     DAZGXS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(31..408)
                     /locus_tag="ACHKG5_001385"
     CDS             complement(31..408)
                     /locus_tag="ACHKG5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFP0867522.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(530..862)
                     /locus_tag="ACHKG5_001386"
     CDS             complement(530..862)
                     /locus_tag="ACHKG5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFP0867523.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            complement(1866..2333)
                     /locus_tag="ACHKG5_001387"
     CDS             complement(1866..2333)
                     /locus_tag="ACHKG5_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP0867524.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            2526..3095
                     /locus_tag="ACHKG5_001388"
     CDS             2526..3095
                     /locus_tag="ACHKG5_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0867525.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            complement(3294..4610)
                     /locus_tag="ACHKG5_001389"
     CDS             complement(3294..4610)
                     /locus_tag="ACHKG5_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867526.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4762..5514)
                     /locus_tag="ACHKG5_001390"
     CDS             complement(4762..5514)
                     /locus_tag="ACHKG5_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867527.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKADVTQQMIT
                     LYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5637..6095
                     /locus_tag="ACHKG5_001391"
     CDS             5637..6095
                     /locus_tag="ACHKG5_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0867528.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            6811..7593
                     /locus_tag="ACHKG5_001392"
                     /pseudo
     CDS             6811..7593
                     /locus_tag="ACHKG5_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            7713..7900
                     /locus_tag="ACHKG5_001393"
                     /pseudo
     CDS             7713..7900
                     /locus_tag="ACHKG5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8098..8949)
                     /locus_tag="ACHKG5_001394"
     CDS             complement(8098..8949)
                     /locus_tag="ACHKG5_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP0867529.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYQNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(9023..9265)
                     /locus_tag="ACHKG5_001395"
                     /pseudo
     CDS             complement(9023..9265)
                     /locus_tag="ACHKG5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(9490..10593)
                     /gene="zorE"
                     /locus_tag="ACHKG5_001396"
     CDS             complement(9490..10593)
                     /gene="zorE"
                     /locus_tag="ACHKG5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFP0867530.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(10597..11304)
                     /gene="zorB2"
                     /locus_tag="ACHKG5_001397"
     CDS             complement(10597..11304)
                     /gene="zorB2"
                     /locus_tag="ACHKG5_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFP0867531.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(11311..12963)
                     /gene="zorA2"
                     /locus_tag="ACHKG5_001398"
                     /pseudo
     CDS             complement(11311..12963)
                     /gene="zorA2"
                     /locus_tag="ACHKG5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
     gene            13175..16534
                     /locus_tag="ACHKG5_001399"
     CDS             13175..16534
                     /locus_tag="ACHKG5_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFP0867532.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLSRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRGSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            16534..22848
                     /locus_tag="ACHKG5_001400"
     CDS             16534..22848
                     /locus_tag="ACHKG5_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0867533.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPRKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            23072..25930
                     /locus_tag="ACHKG5_001401"
     CDS             23072..25930
                     /locus_tag="ACHKG5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0867534.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAGFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            25933..30867
                     /locus_tag="ACHKG5_001402"
     CDS             25933..30867
                     /locus_tag="ACHKG5_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867535.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            30867..37208
                     /locus_tag="ACHKG5_001403"
     CDS             30867..37208
                     /locus_tag="ACHKG5_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0867536.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            37205..39319
                     /locus_tag="ACHKG5_001404"
     CDS             37205..39319
                     /locus_tag="ACHKG5_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP0867537.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRCLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
     gene            complement(39995..40690)
                     /locus_tag="ACHKG5_001405"
                     /pseudo
     CDS             complement(39995..40690)
                     /locus_tag="ACHKG5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(40813..41793)
                     /gene="nanS"
                     /locus_tag="ACHKG5_001406"
     CDS             complement(40813..41793)
                     /gene="nanS"
                     /locus_tag="ACHKG5_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0867538.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            complement(41858..42964)
                     /gene="nanM"
                     /locus_tag="ACHKG5_001407"
     CDS             complement(41858..42964)
                     /gene="nanM"
                     /locus_tag="ACHKG5_001407"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0867539.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(42984..43700)
                     /gene="nanC"
                     /locus_tag="ACHKG5_001408"
     CDS             complement(42984..43700)
                     /gene="nanC"
                     /locus_tag="ACHKG5_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0867540.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
BASE COUNT        11552 a        10028 c        10776 g        11902 t
ORIGIN      
        1 aattttttta ggggaaaatt atttagttag tcacactccg ccagtcaatt ctgcgattag
       61 tggttcataa ggtgcgccag cttgtagtgc attccagatt tctaaaattt tatcacgatt
      121 tctccccaac caactcgaaa catcactatc atatttctta ggtaaatctc cctctatgcg
      181 gtctcctgat tgtatggcat aactggcagc atgcgcttgt tttccatagt caatatgaat
      241 atggggaagt tgatgtcctt tttcttgata tatttttatc ttcatgttct gtctcttcac
      301 caagagaatt tccatgaatc cgccgccact agagcgtctt ttctcagtca gtagatcctt
      361 ctgagcaaag agtttttgta attcgtaaaa ttcttcatct aaattcatat atcacccatt
      421 aaatttcagt tagtagttat taaactcaac tctttatcgc taggcttttc ctagtccact
      481 tttggcacaa agctgtttag gtcaaatcta agctaataca cttgattact tacgattgca
      541 tccgcagccc tttcacgctt tctgatggta ggctaatcac tgttttaatg cagttctcta
      601 tgctgccaat gatgtcttcg cgtgggccgc tgcgccagtc tgcgcttgcg taaaaagcat
      661 cgagaaccgc agtcatgctt ttagcgctgt caaaggcgcg aatcaagtag tagcaatcca
      721 ggtcatcaag cgagttgcca aacgaaacaa catcgatccc gtggctttgg tgtaggggaa
      781 cactgatttc ctgcattatg gcgtgaaaag cggcaccgct gcctttgcgt aaggtgtatt
      841 gcagtatttc gacggttctc aagcgttatc tccgtgttgc ccttgggcgg ctcctagttg
      901 atagcaataa agtaaaccgg actgggccat gaagctactg ctaatcagac agtcatccgt
      961 aagcagaagc tgcgatatgc ggttcaaacg cgctgtaagg ctctctggcg agtgttttaa
     1021 agaaggtagt cgaaatcatg gtgcagaatt cgcggcgctc tcatagagtg actggtgaag
     1081 ccagttgaaa caagcgtagc ggagttcgag caatcaatga gaaatctggg cgagctttag
     1141 ttttaccgcg tcgcttaact ttggaggctg attgcctcta taaggactgt ctcaccgcca
     1201 cggaaattat cggatgcaat gcttaagagg ggcgcttcca aacctaggac atccactttt
     1261 tcgctcccaa cttatccggc tgcatttaca ccgcgaacac gttgaacgtt agcagcttct
     1321 ggttcgtgtt tggttgttac gcatgtgagg taaaaacagc agctttactt ttgacgggaa
     1381 cgccataaga caccaaacag cagagaagtg taattgttaa aaatgttaaa acataacttg
     1441 ttctttaaga tgtaaaccct tcctcggggg ggaagccgga catatcaacc ctaaggccat
     1501 ctgagaagat agcctgtagg tttgctcggt tccccttgcc agatgaagat caagtcttcc
     1561 tcgtggtgtg acgacctttc ttgaaagatg cattatgcgc aaaagaagct acttaagaat
     1621 ccgccgatag ctactttcac cctctgcttg gctgcatcaa acgcttgcgc gccctaccta
     1681 ccacaatcaa cgttacgcag agttcccatc ctgacgtctt ccgctaaaca gcggtgatct
     1741 ccacctaccc ggaccggttt accgcatgag gtaggtgtca ggagagaagc tagcacacca
     1801 tcattttaaa gaaaagtcat atatatcata tggctgcgtt catattctga acgcagttat
     1861 cgatgttaac ctaaaggtat gagaacactg tcgtttttca tatacaaagg gtgcttcggc
     1921 tcacctgtag cgtttaagac gaagcacatt gtgctctctg gcaatagctg tcggacggtc
     1981 gttgagcgtt tgaggtgtct tccttcattt ccccatgcgg cgacaaccag tccggcaccg
     2041 ctaacaagac gtatcaagtg gctatcgtta tctttgccaa tgggatcaga cgctttcatc
     2101 atttcatgtc gctgggtgtg aacgaatgcg aacaggttgg ccatgtatac gccaccgtag
     2161 ccccagcttt tggcaaacga tatgcagcgg ctcaaggtgc gatcatcctt ctcggcatcg
     2221 gcgtatgaag ggtttagccc tatgaaaagg gtatacggtt ttgattcgtc ccagactcgt
     2281 gataacgagt agcgatactt accgcacggt gaaaaaatcg cgtctttaat catttcggtt
     2341 gtccttatga agtgatggtt tcagcctaac atgtcttcac ccgttttaag cgccaacaaa
     2401 cgaccgtttt ccggcatcct ccaaccgcac gctttcacac tgttggaaaa ttagccttgg
     2461 cttttccggc aggccatcct attttcatca ccgttttctg gcaggggccg gatgaggaga
     2521 tgactatggc tatttttggc tacgggcgtg tttcgacatc ccagcaggat actgagaacc
     2581 agcgcatgga acttgaacaa gctgggtgga cgttcgactt ttggttttct gatgtagtca
     2641 gcggaaaggt tcctgcggtg caacgcaaag cgttttttga aatgctcagc aaaatccgcg
     2701 atggggaaac gttggtagtt gcaaagctgg accgcctcgg gcgtgatgca attgacgttc
     2761 tgcaaactgt caggacgttg gctgaccgaa acatcaaggt tattgtccat cagcttggca
     2821 ccactgatct cacctctgct gccggtaagc tcctgttgtc gatgctagca gcggttgcgg
     2881 agatggagcg tgacctgctg attgaacgga ctaatgcggg gctgttacgg gcaaaagctg
     2941 aaggcaggaa attgggcagg cctgctaaaa tcgcaccgga agctcgtgga gcgattttag
     3001 acaaaagggc cgctggggtt agtgttagcg cattggcgcg tgaatacggt gtttcaagag
     3061 cgacgcttgc tgcattactg aataagggtt actgatatac ttaaaacatg ctctaacact
     3121 ttgcagatac gagcaatact tggtaaaaca gcacactttc cgccagaatc ggatttttga
     3181 ggtcctggca acagctaatt gctgagaaca tttcagtaaa ctgtttaact cgctatcagc
     3241 cgaacactga tttgtataga aatgctaccc tcgttcatac tatcaaaaat aaattaattt
     3301 tgatgccagc caggttggtc attctcaaat acctcagcct cggggaatat tttagtcaat
     3361 gggataacat atttgaccag atcatgggta aaaaagtcat ccgcagtttc atctaccatc
     3421 cacgcagagt tctcatctat gcgtggcacg agataatgaa cattcaggcg gactatttta
     3481 gatttttgct caaaatttac tatggcagca tagttttttt cccttcgttt atcggcaaca
     3541 tccttttgaa tatctacatc caaagctata tgccctacgc ctagaccata atccaaaagc
     3601 tggttatcta tctctgctaa tgtttttgtt acatgtcgcg aacgtgcatc gaaagatttt
     3661 tcattgatac aacgccatgt tatcaaagaa gcaagtttaa ctttggttat gaaacgtgag
     3721 tcgcgctcat ctggtcgtcc catagcaaca acttggtaat tttcatcttc cagaggggag
     3781 cctttgataa gacgagcaag tttagtgttg actagtaatg agccgttttc agtgatatct
     3841 tccctgataa gatttaagtt ggccggtcta atcgttcctt ttccataatc atcattccat
     3901 tcataaaata cacctttaaa gttttttaag tggctaatga tatagttttc aggcgcgtct
     3961 ttaacttcac ataaatatgt gacatcagtc catacgttca tctttttgga tttgatatga
     4021 agttcggcaa gatgtgagcg tatatgatgc tgtaatattt cctgctttac gtaaggacct
     4081 ttctgaagcc ttttgcattc gacaaagaaa taatcgccat ctccaaggcg gcaacgaaac
     4141 tccggggttt ttgctatccc tttttgttct ggaatgaact caacttcata accttctgat
     4201 gcatagtttc cagcaagaac caactctaat aaggctgtgt ctggtaatac tgttgtattc
     4261 ttaagcattc tgattgcgcg atctctagcg ccagtaattc tatcgagcga tttagcacac
     4321 actcctaatt gtttaaccca tggtattata tttgaagcat tagtaacttc gtaagacctt
     4381 ctattatcga taagcgattt tgcttgtgcg aaataaccag caaccacgtc ggaaccatac
     4441 cattctgggt caaactgttt cccgaaattg gcagcttttt gggttgtagc tatgtaaaat
     4501 tgctgtgctt ttgctaagcg ctggtaaaag tgttgtttgt tctcttgagt tgatgctaac
     4561 cactcaagac cagcaataac gtcagaatct agcataacac cgtcattcat ctttattcct
     4621 tagttcagcc ctgaaaggca ctagatcaac tgagacagat tatagttacg gaaagataac
     4681 gcgatgaata tacgatggta agcctccgct ttccactcta tgctggcagc tgagttctat
     4741 catgttcagg cacctacgtt tctagaagtt gttcatacag ttagtataaa tatacgtccc
     4801 tgaaatcttt gcgtcttgct ttggatgttc atatacgtag tcaacaaaag tcttatagag
     4861 cgttatcatc tgctgggtga cgtcggcctt accgttcatg ttatcaagtc gctgataata
     4921 ttctcttttc gtcatcccac tgttacggcc ttcggttacg tttgcggtaa attgagccat
     4981 acccatgcac atttgatctt tattgttatt cgttttatcc gggtggagag cgccttccaa
     5041 cactttatca ccgctgatgg catgttgcgc ccacgctgaa ttgttagcga taaacttttt
     5101 ggagccgatg gcaataccgt attcaacgcg aattccattg tcgtgataac tgccatctgc
     5161 gtcttttgtc gctagcttta aagatatacg gcaaccggga agcgttttgt gttcttgcac
     5221 cgagataata acggtgtccg tatcgcaatc agcaaagctg tattcggggt taacgtaagt
     5281 gttagttttg attaacagcc cggtaagacc gtttttatag ccttttgccg gcagcgtttt
     5341 gaaggcgctg cgttcaccct ttttgatcgc ttcccggaag tgatccatac cgatgcgcgt
     5401 cgccagttga tcgatgcttt ctgctgcata agatgcagat gacgcagcga ttaacataat
     5461 ggtcagcaca gacttggctg aaaaaccggg cttactaagc cgtgtttttg acatgttgtt
     5521 atccctcaga atccacccca gcggtgtcag ttatcgcatc atagaccatt gattcaaaat
     5581 gtcactattg gagcgtggct ctttagtgga catctgcgca aattcaggtc atgaaaatga
     5641 ccctgagaaa actgtttgga caacgagtaa aagagttgcg cattgccacc ggaatgagcc
     5701 aagaagcctt tgccgatcgg tgtggttttg ctcgtagcta tatgagccgc attgaacgcg
     5761 gtggctctaa tgcgtctttg gatgcgattg aggtgctggc taatgctctg agtgttgagc
     5821 cgtggcgact attggtgtct gattcatctg aagatagtga cccggaatta ctggttcctt
     5881 atgcagctga tggctcgtgt tttcatcccg ggctggcaag cactcgcgat ggctcgtttg
     5941 gcgtaggtga taaaacggct cagaagcggt ttggcacgtt ttctgaagct cttgagtatc
     6001 ttcgtagcat ggagacagca aaatggcgta ggcctaatcc tagcggcaac tggggtattg
     6061 tatctgcggt tcggtgggac aagctgagaa agtagctcaa gtgtaagttt atgaattttc
     6121 atgctgtatg gatgaacagt gttcattaga tgctgatagc ctcacgtttc atcagctctt
     6181 tgtttctata agagtgaata aaacagcaaa atgcgggtgc gttacgtagg cgttacggtg
     6241 gcgttttact ttgacgctga atggcttaat tcatataaat aacattgagt taaatagtta
     6301 ttctgaacaa ggtctgacgg ggtttaactg gtttcaaaat ccgaacgcag tggcgtttac
     6361 ttaacatcca catagctgac aaatgttctt tcctcccgac ccactagacc tccataaaca
     6421 tcaaaaggca ggtatggcac gttaatgatt tgtgccgcca cgaacactcg gtttgattcc
     6481 gtccagcgga tcaaacttaa gatgtagccc ctagaacaaa aaggtcctac gattgttctt
     6541 ttaaaggcta ataggaacgt gattacagca ggttatggag acttaggttc gagtccggcc
     6601 ttcggcacca aaagtatgta aatagacctc aactgaggtc tttttttatg cctgaaatcc
     6661 gcgccacgca aggcttttcc agcttttctc ctcaactgaa gtcaacctga accaacccac
     6721 atcaacaggc ttgtgagtat actaatgagt atatttgccg attcgatatt gcttgtatac
     6781 tcacgagata ccaatggaag gaagaccatt atggccctaa cggatactaa agttcgctct
     6841 gcgaaacctg aggagaaaga gtattcactt gttgacggcg acggcatgtc cttacttgta
     6901 aaacctggcg gttcaaagta ttggcgattt cgtttccgtt ttggcggtaa acaacatttg
     6961 atggcgttcg gcgtttaccc ggatgtttca ctggcggatg cgcggaagaa aagggaagag
     7021 gccagaaagc tggtagctgc tggtatcgat cctcgtgagc ataagcgtgc tgtgaaagaa
     7081 gagcaggcga aagagattat tactttcgag aaggttgcca gagagtggct cgtaaccaac
     7141 caaaaatggt cggaagatca tgctaatcgt gtaaaaaaga gcctggagga caatatcttc
     7201 ccgacaattg gtactcgcaa cattgctgaa ctaggcaccc gtgatctgtt aattcctatt
     7261 aaagccgtgg agaaatccgg gcgtcttgaa gtggcttccc gccttcaaca gcgcaccacg
     7321 gccatcatgc gttatgcagt gcaaagcggg ttaattgatt acgatccggc gcaagagatg
     7381 tcgggggcgg ttgcttccag taatcgacag catcgtccgg cgctggagtt aaagcgtatc
     7441 cctgagttgc ttgacaaaat agacagctac accggcagac cgctaaccca ctgtacgaca
     7501 gaactcactc tgcttatctt tattcggtcc agtaagctgc attttgctcg ctggtcagaa
     7561 atcgattttg aaacgtcaat gtggaccatc cctcttgatt ggtattgcag taagcagagg
     7621 gttggactgg tacagtaaat atcccgcata atcgtgccat tcacatttag agatcctccg
     7681 gcataatcaa tctgccaatg aaggagatcg ctattcgtaa agcccctttt actgagcatc
     7741 agattatcgc cgtgattaaa tcgcttgaat acgggcgaac tgttaaagat gtggcttatc
     7801 tgaagccact tactacaact gcaagtccag atacggcggt atggaatttt ctgatattaa
     7861 aataatcaag gatcttgagg atgagaacca acgtttcaaa tagatgtttg ctgatctcat
     7921 tatttggtcg ggatttttaa agatgtatgg aactaaaacg ggtctatttt cagagggatt
     7981 taactgaagc agaaattaat aaatcataat ttataggtgg ctggtattta atattcctgc
     8041 atcatatctc tgatgcagga gcagagaatg gctataaatt attagtaaac agaatgtcta
     8101 tctctcaccc acaaacccct cataagggta atttttacgc acctgcggca acgcaatagt
     8161 tttcccgtca aaatcccaga ataatctctc aacgatccct ccgccgttta ccgcatccga
     8221 gaccagtacc cgcatatttt catccaggcc aatcgatcct ttatcaaagg ctttatggtg
     8281 tattgcgcaa agcgccagac cattggggat ttcacaaggg ccgccgtgct gtttccattt
     8341 tatatgcgcc gcttccagcg cgacggtggt gtcatcgtgg cgcatattaa agccacatat
     8401 tgcgcactgg tagttataag cgcgtaatac atttttgcga aataacgggt cgcgctgttt
     8461 acgggtttgc tggagatcaa aacccagctc gtcagcgatt tcttcctgaa tactctcggt
     8521 gaagtgcgct tcgagtatct gttgcgccag agtattgatg agttttttat tgccggttac
     8581 cagggcgtag tgctgttcat cgaaaccgcc tgcaacgtgg tattcgttca gttccttcac
     8641 tggcggttgt cggctgctcc ctgctgttga gcaacgctcg gcattgtgta attgccagat
     8701 tccgtcgcct tgtaatcgcc agaacggcat atcaggtcgg tactgtgaac gctgtggccc
     8761 aaaacgttcc agtaaactgt gcagaggttc gtagatttcc gtgccataat cgaaaaggcg
     8821 cggatgtcca ttctggtatc ccgctaatac gtatagcagt aacaatggtt tatgcggcgc
     8881 gcgctgttca cccttgtgcc agatttttat gttggcaatt gcctgctgta gcgatttact
     8941 ggaagccatt gatatcccag gcagtgaaat aattgcaatc atgctcgcaa attttctgct
     9001 gttcaagcct gcaagtgaag atccattgct gtgttgaggg atcaaaagac aacaaaaact
     9061 tattgaagaa ataatcttct gcttttttgt cgaaataatg ggcattgatc ttgtctatag
     9121 ctgctgaatc ttttccagcc tcgttgagat cttcaaaata gccattgaag atgttctggt
     9181 taacaccgcc agtaacataa gccttgccgt tgtgtacaaa agtcacatta cccgccatgc
     9241 ccatcggcgc gtgcgacatc aatttattgc tttattcatt gtgactgtct tctgtctacc
     9301 aagctttaac cttcacccat atgagggaag ttttaagtat gaggcgaacc atagcatatg
     9361 cttttatacc tgttacacca taaaaatcag acaagttatg ccataaacta cttttgtaat
     9421 ttgagccaca aatcagggaa attttattta tcttccgtga aagtcttaac tttcacggag
     9481 ttttagaatt tacaattttg ctggcgtaaa ggctcctgtt tgtatttctt taaccagctc
     9541 acccacggta tatattctcc atccttcgac ttcggcggga gtaaggttaa ctgcaatttt
     9601 aaggcctggc catgcagctt ctacggttgc aataatttca tgttctgagt ttggaagatc
     9661 taacccagga acaggaggtt gaaagccttt atgttgcatc atattaatat caccatgcac
     9721 tgatggatca gtcaggtcat atatttcatt ccaggattct gcttttaata acccttgttg
     9781 ggaacgtaaa cgttgaatga tagccatctc tgcttgggta acgaatatac cttcgtggag
     9841 gggctgtttt cgatggcaat ccttgcacag aacaagaagg ttttcatggt gattatcata
     9901 tttgatgcca tttttatgat ggacatggca caagtttttg gcggttgata aattcacgcc
     9961 acaatcatta caaacataat ttgccttttc tcgtatttct ttagatattt ccttccagtt
    10021 ttcgacatac ccactatttg ttttgtcata gataccctta ggcatatgac gaaaacaggt
    10081 actataatct gaaaaaaact catttaatga gaatgattta aaaatctctc tttttcgttg
    10141 tttatcaata cttcctttat aattaagtcg ttcgaggcaa ttcatacata cccgtaaatc
    10201 aacatcctgg ctccgacctg aaccatcatc aatttcgaat aaaccatcta tgcggttagt
    10261 tgcatgatac ctttcaaatc gatttttatg gcgcatttca tcaagagttc tgcaccaggc
    10321 aatatgaaaa cgttttcctt tttcgccatc tacgatagcc gcatcataac gccctgaatg
    10381 gtcccgaata tataaaagaa cctggcggcc atgataagag ataagcccgc taccagggtc
    10441 aagatcttcg atactaactt ctttacccgt ggataactca atatctatag gatcaaagtc
    10501 aataggggat tcatccagaa aaaaatcgac tccttctggt aacattttct tccctaattg
    10561 aataagttct gaaatgtcga tagataattt catctcttac tcctgaataa tctttctaat
    10621 ctgcaactcg gcatttgtta caactttgaa ggtgacacga cgagagcgat taggatcctc
    10681 cttgccggtt ttatcaagaa tgggatgtgc agatgaataa cctactgcgg caaatttact
    10741 tttaacccat tgttggtgtg tcgcgatatt ttttatgtca taaacgtatt gtaatactgc
    10801 acgtgtacga ccttgcgata gtgccatatt attaaaataa gcaatatcag gattcgttgt
    10861 tcctgtccag tcagtacttg tgtgaccttc aatgcggact tcagtaatat gttcctgata
    10921 attatctaga acttttaggt agcgaggaaa gaagtcgtca agaatgagtt taaactttgg
    10981 ttttagctct gtgcttccta agccaaataa aacatccggt gatttaaatc gaacctccag
    11041 agtctgttta tcgatctctg catcccagtc ttgtaaatcc tttgcaaact caatatccag
    11101 agcattataa agctgtaact gagcattctc gtaggctacg gcaacttctt taattttttc
    11161 tttttcaata cgtacgtagt gcatataagc aatagatatg aacataaaaa ccatcatcag
    11221 ccctgccatt aggtccgaca tggataccca atgttcatta tcttgatctt ttttaggtaa
    11281 ttgtttccct ataatcttat ccatgatcga ttacccgcga tgtttattag cagaaattaa
    11341 tgcttgtaac tgccccataa ctatttggta atctttaata agtttttcat agttaccgac
    11401 aaaacctttg ctgattgaaa gcagagcatt acctaatgat tgcatttcac ggttcatttc
    11461 acgttcagtt gcttgctcaa aagcatgtaa ctgctcgttg atggatagag caaatttttc
    11521 aattgattct ctgacttgag aagtcagccc tgcacttgcc ttatcaaatg atgtctggat
    11581 tgaatcaaac atattatcgg cattactgga tagttgatgg ttaatctttt caccagtctt
    11641 actgaacata tcaatcacat tcgtcgtatt ttgttccata tttctatgca attctttcga
    11701 gtgcgactct agtgatttca ggaattcttc accactttgt ttcatttcag taattgtttc
    11761 acctaacgtg ctggttaggg tttcggagga attgctgaca tcatgtgcca tcgaggcaaa
    11821 tgcttgttgt gtttgggtaa ccgattgtct gaatccttcg gtaccttcat ccagggcaac
    11881 gcgcattgaa tctgcattaa gaagtatttg ctggctggtt tgctcaagag atgcactaac
    11941 atttgcagct ccggatttca gcagttcacc cacttcagcc attttgttct gtatttcagg
    12001 taatacggtt gtagctttat cgcggatggc gacaaaggtt tctaaatggc gggagagttc
    12061 gctgatttga tgttggttca cctgaagcac ttcacgcagt tcagacatag ccagaggaat
    12121 ttctttacat tcttcccaaa tccctgcaac cgcagttttt gtttcaacca gggactcgac
    12181 actttgttga tattgttctg acatctactc aatttgcgtt ttataatttc cctgccactc
    12241 aacaagtttt tttacagagg catcaagagc tttaaagttt tcaccaaact gttcagttaa
    12301 attttgatta aaatcaataa tgacttgtcg caaagcatca ataatttgtt ctgtagcacc
    12361 ttttgccatt agatctgcaa attgttcaag ttgttcccaa agcttattac tgaaatgagt
    12421 gtgattagct aattgtgcct gcgaggaatc gctaatctca gtacgtagta attttatttg
    12481 agcaataaga gaaccttcac tatcaccgtt aatacccgag actaatttag tcagagtctg
    12541 gttctgctct tttaattcat gatagatatg ttcaggtgta acagattcag ggttattttc
    12601 tgctaacgca cttcgtttat tggcaaaaaa gaaagcatcc attccattaa ataaaatggc
    12661 aaaaaacata cctacaatgc ttgtaatgaa tgctgttttt aggccaccta ataatacggg
    12721 gatgctggta tcaatacttt cggtattaaa attgagtaaa ccaataataa taccaatgaa
    12781 agtacccaga atacctaatg cccccattaa ggttggagca tattctctaa acttactatg
    12841 tatatttttt ttgtcacacc accacgctga taaaaatacg aatataacag cccagatgaa
    12901 aatattggtg atcagtgtag agtctatcga ttgaaacaac tgctcaaaaa gctgcgctaa
    12961 cattttgata cctgtgtaaa taatggatag ttgtaatcgt ttaaaaaata aacaaaacac
    13021 acataattta tatggttaag ttcggtgtag cgctcatgat tgtaacaagt tttttcgaga
    13081 tataaatgat ttgtctcatt tttagcgagt tcatcgggtt gagctttttt atgatgatag
    13141 taacctgtta cgaattgtat caaaggaagg caggttgctt gtgaaaactc cagttaaccc
    13201 acttttgcaa tggcttaaca tgttttttag tcgccgttca ttatccggag ctgatggacg
    13261 ggcgttatat gcttaccgtt gtactgatac agagtacgaa agtttggcag aactactgcg
    13321 tacatatgcc ccccgtagtt atccaagaac gatattcatt tcctatagcg atgttctatt
    13381 tagcatatat gccgcagagt ttatccgccg gactcatacg gtcggacacc ctaaatggga
    13441 cacaatttta gattctatta actggaaagt tccgtatgtt catcgacaga agctggttaa
    13501 tgatggcatt cgctactgga aaagaaagat aagaaacctg ggacaagctt cgggttatct
    13561 gcatacactg gcttgtgaag gtggtttgcc tatccgcatg attgaaaatg agagcggtta
    13621 tttgattacc tatttcagaa ggatatatca ggcgcttcgt gggcaatcat ctcaatatcc
    13681 tgccgctaaa attgcacaag aattaggcga tacgattcct gtcacaatgc aaaacgaact
    13741 ggtctatgaa atagccggtg aattctgcga gactctctcc cgattactta gtgagcatcc
    13801 tcctcatagc agtgatcccg tttctgcatt acgtaagctc tctccagact ggcatcttca
    13861 acttccactc gttctccccg aagcgaatgc tgcagagatt gtaaggcgat tactttctca
    13921 atcttctgaa atacgcagtg caagtagtct tcaggttgaa agaatctggg tcgatgttga
    13981 tgacagttgg tattgcgatg cccggttccg attcccggcc acaatgcgta cagaacagtt
    14041 aacctctttg tttgaatgcc atattcagcc ggagcagacc cgactcatca tttcaggaaa
    14101 atggaaaaat ggcggggcaa ggttggcaat gctaagccgt tatgagcagc aagattggcg
    14161 ggtcgagtta ttacctattg cgatgcaaaa actctctggt gcagatgcaa tggccgaaat
    14221 ctcattgtcg cttcatgaag gtccaattct gttaggtcac acaattccaa aaggcggtta
    14281 tgaacttact gaagaacttc cctgggtttt tgaagcgatg aatgagagtg aatcgcagct
    14341 aaaacttgtt ggtatggggt ctgtgagttc caggctaaat gctctgttta tttcgctacc
    14401 gaaaaatagc catttagata ttagtggaga aggtgagttt gatatcccaa ggttgctgaa
    14461 aaacagtgaa cgcagcttaa ctaaaataag tggagtattt agcgttgttt tacatgatgg
    14521 tgctgtttgt acaattcgca cccagcaact ttatgattct gcgattgaat attatattaa
    14581 atcgacagaa gttgaactgg ttaaatcgga ttatcctgtc catcgagcct ggccgaagat
    14641 cggttggaaa aaggatctgc aatatggcat tgtaccggag aaagaacttt tttggcgttc
    14701 cattcgttca ggtaataatg cctggtattc tgtcgcatca gaaatgccaa aaggacagat
    14761 agaagtccgg cgaatagtta atgatgaggt tttatttagc ggtaaagttg tagttttacc
    14821 agcagacttc gatattaata ttatacctga gagtgctcag caaggcatta ttatgctttc
    14881 tggtataacg gatactagaa ttgataaata ttcaaacaat gaaaaagtaa cacttaagtc
    14941 cgattattca caaaacgagt gtgctattta ttataattca tcgctgatgc tggaaaatac
    15001 cgttgattta cggggctcct ggaaagatgg ttctaatctt aaactattat tacctaagcc
    15061 ggttagcggt ggccgatttg taactaatga tggttctgtt cattttgatg gtgtggcatc
    15121 tatagcacat ttgcatggaa tagatgctga gttattaacc atatcatgtg ctggaagagg
    15181 atatcttaat atcgagttat tggatgaaaa tccagtagct gaaaaattcc gctatttaca
    15241 tgccgacctt cctcttttat caggacgcaa tgacaaatta caacaaattt cactttatga
    15301 gaactataat ttgctaaatg ctatgctggc atgtgcatgg aacagcaata gtacattatg
    15361 tgttgatttt tactctgaca gatttggaaa ggataaagca acactcaata ttaaacgcta
    15421 cgatggtagt tttattgaac acgatcaagg gttactggtt gatataaaaa attctgttgt
    15481 ttttcctgca aatagaatag acgaactggt tgttgatgct atttctctta agaatcctgg
    15541 cttacacata tcattgttaa aaaaagatga gtttgcttat gatctttcag ctctgaatgt
    15601 tcaggatagt ccatggttaa ttgtgggaaa acttgatggt acagctcgca ttgcaccagt
    15661 aattaaatgg atgctacctg tattgcagac aaatgattta ttactaaatg ctctatgcga
    15721 agcagaccca gaacagcgca aaaaaaattt taatgagcta atttttgaaa tagataacaa
    15781 cccattgcaa aattattgct gtttattaac agagtatatt aagaaataca aaatgaataa
    15841 tggcttatcc ttgctggatc tggacttgtt cagaggtatt tcgagtaatt accgcgtggt
    15901 tgttcaattg ttaatatcat catgtctttc tggtgatagc gatacgattt atgatataca
    15961 ggaagaatta cccttttcat ggggatggat tcccgtttca atctggaaag atgttttcca
    16021 aaaatgttgg acttatctgg agaaacagat taacgataaa acattagcat tacatatatt
    16081 gcaacccttt attgctttta tgaaccatcg tgcacatatc gatcgtcggc tggctccgat
    16141 tgcgaatatg ttacttacat atagtgagag cctaccaacc ggttgtgatg tattgccaac
    16201 tgttagtcgt gagcagttta atgaagctaa acagatgcta ttaaggaacc ccgacagctt
    16261 tgggcgtatc agtatcttcc ctaaagaact ttggtctagt gctattactc cagagttaaa
    16321 atctgttttt aataagcttt ggattgaaga taaatatcac tcacggcttg aaaaacgttt
    16381 taatttgatg ttagtcgcag cgctgttaac ccaaaaagat aataacctga tacatcaact
    16441 gtctgcgctt tttgaatttc actatcagca agccccacag caattagggg taatctatca
    16501 atattatttt gaacaagcag gagtatgtca ttgatggacg ttcagcaact gcattatgcg
    16561 ccacttctaa atcagcttca tcagcgtgcg gtgaggtcgg tggtaagtca gttggcattg
    16621 cggagcaagt cgttgactca ttatctggaa tcacaatatt ttcagcctcc cggtcaggag
    16681 gggtcactgc ttgcagaccc ggtattcgaa agtacattcg gctggatgcc tgcggatgaa
    16741 acaatggagg atttgcggga caagctgatc tccagcccgc tggttgatgc attgagcaaa
    16801 gcgccagtgc catttcgtca tcaacttact gcgtggcgaa cgattctgga agacaaaaaa
    16861 tccttgttag tgagcagcgg tacgggatcc gggaaaacag aatgctttat ggtaccggtt
    16921 ctggaagatc ttattcgtca gcaaaaatcg acccgccagt cactaacagg gacacaagct
    16981 attttcttgt atccgttaaa tgcattaatt aacagccaac aggagcggct tcgcgaatgg
    17041 acgcgcggat ttaaggggaa aatacgcttc gcgctatata atggcgaaac ccggcatacc
    17101 aaatacgaaa ttcaggaaga tcagttgaaa gtacctgaac aagctttgag tcgggaagcc
    17161 atttatgaag caccaccttc catcatggtg acgaatacca ccatgctgga atatatgctg
    17221 attcgtcgta aagatgcccc aatcatcgaa aagtctcagg ggatgttaaa gtatatcgtc
    17281 ctggatgaag cccatagtta tattggttct caggctgctg agttagcgct gcttttacgt
    17341 cgtgttatgc aggctttcaa tgttggaccc ggcacagata aacctgtgca gattatcgcg
    17401 acctctgcca ctattggtga agactctcct gaaggtaata aggagcttgc taaatttgtc
    17461 gctgatctcg cgggcgttac agaacgtgat gtcaaagtgg ttcgcggtta tcgacaaata
    17521 ccgcgggtat cggagtcgct gattcgtcat gaatacccca catctttaac ttctcttaaa
    17581 tccctgagcc ctcaggatct ttaccaacag ctttgtcatt accgtgtggc ccagcaattg
    17641 cgtcaggcgt taactcatcc cggacggcag gcagtacgtt tgagtgagtt gctcaatgtt
    17701 gccagaagaa cctggccaga tattaatcac cgtgagctac tgcaactcct cgatctaatg
    17761 gcaagatcgc gagaaggtga actggctttt acgccattac gtatgcatgg gtttatcaga
    17821 acactggcgg gtctatgggc atgttcaaac aaacaatgct cacataaagc acatgaatta
    17881 aatcagagtg actggccatt tggtcaggta tggtttgagc agcgccaata ttgtgactgt
    17941 ggagcgccgg tatttgaagt tttacgttgc tccgggtgtg gttcagctta tttgtctgcc
    18001 aaagaggaga tgcgcggtga tggaacgaac tggttggtag cgcaaccagc cgctgccgaa
    18061 gtggatgagt ttgctcttga tgtagatgtt tattctgaag atgaagacaa cgacgaatta
    18121 gacaataact ctcaatttga tcgtctgatt gcgagcgatg gtgaaaaata tatcatttca
    18181 ctggaagaaa cgacggcggg gaaaattgat agtgaagcgc aaaagcacta tgaaataaac
    18241 ctgttaagac cggaatctcg aggtgaggga cgcaataact cctttgcttg tgtttgctgt
    18301 ggtgacactc aacggaaaaa taatccttta ttccgtccgc ttcggctcgg cgcgcccttt
    18361 tttttaaatg aaattattcc tactctgctg gagttttctc cattaccgca aacaagagaa
    18421 gagcagttag gaccgtttaa tggtcgacgc ttgctcacct ttacggattc gcgtcagggt
    18481 accgctcgca tttcagcccg cttacagcag gatgccgata aagcgacgtt gcgagctctt
    18541 tgttaccagg agctggcaaa tattacagag ccttcagcaa aactcagccc ggcacactgt
    18601 gcactgttag agaaagttat tagccagttt tctgtcctcc ctttaaaaaa ttccatcgac
    18661 gcgctgaaaa aagcgatggc tgcgaatgag gagcttagcc cgacggacat tcaggcactt
    18721 aaaattattg agccgatggt gagttcctta agcacggagc atgctgaagc gatgcaaata
    18781 cttcaggcta actctggcaa tcagcagaag aaaaccatgt catggcaaac gctgtctgaa
    18841 cagctggttg gcagtgtgga ccttgccgat agaatgagag gcagttttaa agaattgtct
    18901 gggcttgatc ttactaaaca acagtttgca gatttttgtt tatacagcga gtttggtcgc
    18961 cgccctaaaa atgcctggac cctcgaaagt ctgggtctgg tagccattca ctatccattc
    19021 attgataaag ttacaacctg cccgcaggac tggaagacat tattacccga tcccgataag
    19081 caattagcgg aatggcgtaa tttattgaag attactcttg atttctttat ccgtgaaaac
    19141 agtgccgttt tttacgaaaa cgagcaatac cctcgctgga tgggggcacg tttcccggta
    19201 aaaatgctcc aggggccaga taaaaaaaac aaagggcaga aacgtgatca actttggcca
    19261 caaatacgcg atcgccatta taaccgggtc attaagctat taatcgcagt ctttccggca
    19321 atacaacctg agcaggctca ctggaaatcg ttagttaacc acttaatgat tgaggtctgg
    19381 gatgcaattc gaccctgctt acgtcagttt gagtcgggtt atcagctgga tattaaacaa
    19441 caagctgaat tttacagtcc aaagcaagtg tggcgttgcc cctatactcg acgtgcgtta
    19501 gacgtcacgt tgctgggata ctctccgtat ttgccgggta gcaaagaaat tgcacctgaa
    19561 aaagcagtgt tgattgaaat gcctgagctt cctgtacgtc actggcgttt gtctggtggt
    19621 ggagaaatcg cccgagaaga acgtctggag tggctggaaa gtaatgcgct gattcaacat
    19681 gcccgcgagg aaggattatg gtctactcgc agcgaccgac tggccctgaa agatagctgg
    19741 tatcgtctgg aggaacactc tgcgcagaga acgcctgagc agaaccagtt taatgaaaaa
    19801 caatttaagt caggcaaagt taatgtgctg aactgttcca cgaccatgga gatgggggtg
    19861 gatattggtg gtatgagttt ggtggcaatg aacaacgttc ctccagcgcc ggcaaattat
    19921 ttgcaacgtg caggtcgcgc ggggcgtcgt ggagagagtg catctgctgc cattacgtta
    19981 tgtaaaaaca cggcgcatgg aatggaggta ttcaaagacc ccctctgggc gttcaacact
    20041 actgccagtg cgccacgggt tcgttttggg agcagtagta ttgttcaacg acatgttaat
    20101 gccctggttt taggtctgtt tttacgagct gaagttcctg atgcgacaaa actgagttgc
    20161 aaatggttct ttgaaggtga tgagagccag tgtcttcgtt ttctccattg gttaaatcac
    20221 caggccgatc agctggcgga taaacttaaa cgccttactc agggaaccgt actgatgtca
    20281 ttgacggcaa cccagctctt gacccgcaca caagcaatga tgcagcaggt tgatatccgc
    20341 tggagaagcc aacttgcgat actgttggaa aatatcgaag cgttgaaggc ggataacagc
    20401 gcgtgggaag aaacaccagc aggtaaagcc atcgcttatc agcttcgtga ttatcgcggt
    20461 gcatatctgt tttctaaact cattagtgaa gcgttcctgc cggggcatgg tttcccggtt
    20521 ggcgtagtca atttcaatta tctgacggca gatgagctgg aaaaacgtcg cgcaataaaa
    20581 gccactcagg ctgaccctaa tgaaggggga gaaagctttt ctcgtcgtat tgaaaaattg
    20641 cccagccgtg atttgcctac agcattacgc gagtatgcgc ctggtgcaga tgtggtattg
    20701 ggcggaaaag tatatcgttc ctcgggcatt atgcttggca aggtgcttgc cagcgggcag
    20761 gagctgtcag gcgatcatca tattccgtgg ttctggcatt gccgtaaatg tggtgcgggg
    20821 gcaaccagta ctacccatcc tgtcgaatgt agtcactgca aagcggatat acagcaactt
    20881 gatgtgaagc gttatttaca gcctgttggt tttgcgacag atattcgtta tcaggcacat
    20941 aatgatgtat ccatgcctgc acaacttccc cggaaagatc cacgtgttct tgtaccgtct
    21001 tcggtgtggg tttctttacc cgatgctggg ctaggacgtt accgttttag ccatagtggg
    21061 gaattgttcc acttcagtga aggggaattt gggcacggct acgcaatttg cctctcatgt
    21121 ggtcgggcag aatctcaaac tcaaccccag cgtacccctg aaaatctaaa gaacccagag
    21181 agggaaaata cgcattacct gttgcgaggg ggcagtaacg atcgtcaggg gagcaataaa
    21241 ttgtgccacg gtcatgtgca taaagatctg tggttgggat atagcagtcg tacagatatg
    21301 gttgagctgc aactcaatga tgataatggc ctgttgatcc gtgatgaggt tgccgcgcgt
    21361 tcccttgcgg tcgctttgcg tgaagggtta gcgcataaat tgggtatcga aaatactgag
    21421 ctgggcgtga cgacgcagca agcgcgcgat atcaatggct ataccggata ttccatcttc
    21481 atttacgaca ataatgcggg tggtgcaggt tatgcagtgc aattgatcga ccattgggct
    21541 gatgtcttta attacgccag aaaattgctc gattgcagtt gcgataaatt ctgtcaccac
    21601 tgcttgctca gctacgacag tcagcattat gtcaacagat tagatcgtca ccatgcatta
    21661 accctgctta ccaatgtacg gttgcagcga cttaacctcg cgccagaata tcagtacttc
    21721 ggcgatggta gccgggttga aactaaccct ctgagtttac gtattgcgca atgcctgaac
    21781 agcgagattt atgactcctg ctcgctggtt cttgcggggc cgcaggaaca gtgggacttt
    21841 gcccaatggc cattatttaa agagttgctc cagtttgcct cctccggtgg gaatgttgag
    21901 ttgctggtgg ccacgccgct ggcaaactta actgatagct ccagacatca gctttccgcg
    21961 ttggcggcga tgccgggagg tcgcttacaa gtgaaatcca ttgccactgc gcagctcatg
    22021 caaggaaagg ggcgctggct tgcgcaggtg actcgcgaag ggcaaagcca acagtgggcc
    22081 gcagacgaca gcgctaccgt tgcacctggt gaattatggg gacaaagtgc gtcttcacca
    22141 gtagtgacac ttaaaggaac gtccggaaaa accttctcgg gtcagacctt aagtgcagaa
    22201 gatttactgc ccgccttacc caccggggcg gtgcgcatca atctctgtga acaactggac
    22261 gggccgctgg aaggttttgg ttcgcgtttc tggtcgttag tcacgcaaca gcacgctggc
    22321 tggaagcagg catttacccg ccacaaagag ataacgcatg ttgagtacag cgatcgttat
    22381 cttaatagcc cattcacggc gcgtttgtta ggtgaaatct taaccgagct ggttgaacag
    22441 ggaatggcgg aacgcgccag cctcactgtt tgcgtcaaaa aattggatta caactctcgc
    22501 caacacgacg ctctctataa cgcgtggctt aatgaggaag atcgtcagca ggttgtgacc
    22561 actttacttg aagaaggata tctggggcct gcgtggccgg gagcaattag ctggttaacg
    22621 ggggataacc agagtacgga gcatggacga gaactgacag tcaccttcag cgatggcagt
    22681 cagcattatg tcctgctcga catggggctt agctactggc gctgtattga agataccttc
    22741 tttgattttg ccctgcgagt accgcagcaa gttgaacggc tggcaaacac gcgagcgcga
    22801 gctgttgccc cgggtaatga ccttaggagt tatattatcg ctggataaat aattgctccc
    22861 ctggggatat cttccagggg agttgaccca gctcactttt taataatttt tgtttgaaaa
    22921 tcatgcgcaa tttatgcgca ttccctccca ttttgtcact ttgccggggg ccttcatgat
    22981 atctttaagg ccctcttttt tatcgcagtg tgccgcgcca catttttcag gatgacgtta
    23041 ccgccactgc cccaaataga cagaaaaacc gatgaataag caaaattatg cacctggtat
    23101 gcgggtggtt attcgtgatg ccgaatggcg tattcgccgg gcggatgaca gtggtgatgg
    23161 tgggtatttg ctgacctgcg atggtatttc agagcttgtg cgcggtaaag aagggttatt
    23221 tctgaccaaa ctggaacaaa aagtagagat cctggatccc gcaaaaacgc atttggtgga
    23281 agatgaatcc gccaattatc aagcggcaca gttgtatatt gaaagtcagt tacgccaacg
    23341 cgtaccgaca gacagtaaag tccattttgg tcatctggcg gcgatggatt ccatgccgtt
    23401 tcagctcgat cctacgcgga tggcgctggc acaaccgcgt cagcggatac tcattgccga
    23461 cgctgttggt ctgggtaaaa cgctggaagc cggtattctg gtttcggaac tgatccgtcg
    23521 tgggcgcgga aaacgtattc tggttctggc ggttaaatcg atgctgacac aattccagaa
    23581 ggagttctgg agccgttttg cgatcccatt gacccgcctg gattctgctg gtttacaaaa
    23641 ggtgcgtaac cgcatcccaa ccaaccacaa cccgttccac tattttgaca agaccattat
    23701 ctccatcgat acgctgaaac aagacattga atatcgtcac catctggaaa atgcctggtg
    23761 ggatattatt gtcattgatg aggcccataa cgtcgcagaa cgtggaacca gttcgttacg
    23821 cagtaaactg gcaaaactgc tcgccgggcg cagcgatacg ctgattatgc tttcagcaac
    23881 accgcatgat ggtaaggctg aaagttttgc cagcctgatg aacatgcttg acccgacggc
    23941 gatcgccaac ccgaaagagt atgaatacgc cgattttgcc gataaaaatc tggttgtccg
    24001 tcgcttcaaa aaagacgtga aagatcagat gtccggcgag ttccccgagc gcaatattgt
    24061 caaattgact cgcctggcat ccggtgccga agaagaagca taccgtcgtc tggttgagag
    24121 ccagttccgt gatgatgacg atgaacaggc ccagtctaac aaaggtcgtc tgttcaagat
    24181 aaccctcgaa aaagcgctat tttccagccc gatggcgtgt gccagcgtcg tggcgaatcg
    24241 tctgaagcgt cttgagagcc gtaaagatca taatagccag agccagatca acgaactgga
    24301 gtcattgctg ttggcgctga ataacattga tgccagccag tttagcaaat accagttgct
    24361 gctcgacacc atccgaaaag acctcgcctg gaaagccaat aacacggaag atcgcctggt
    24421 gatcttcacc gaaagtatta aaacgctgga gttcctcgaa cagcaactgc gagcggatct
    24481 gaaattgaaa gatgaccaga tcgccacgct gcgcggcgat cagggcgata cggtattgat
    24541 ggaaaccgta gaagcctttg gtaaaacgca gtcgccgttg cgtcttctgg tttgttcgga
    24601 tgtggcatct gaagggatca acctgcatca ccttagccac aaaatgattc actttgatat
    24661 tccgtggtcg ttgatggtat tccagcaacg taacgggcgt attgaccgct atgggcaaaa
    24721 acatcagccc caaatccgct atctgcttac cgaggccagc gaaccacaga tcaatggcga
    24781 tatgcgcgta ctggaagtgt tgatcaacaa agacgaacag gcgcagaaga acattgggga
    24841 cagttcggag tttaccggca aatttaccca ggaagaggaa gaagagcagg ttgcagggtt
    24901 catgatgcag gatgatggtg ccagcctgtt tgatcaactg ctgaacagca acgtctcgga
    24961 aagcgccgaa cacgatctgt ttggcgaaat atgcagtgcg gtttccagcg atgcctcaat
    25021 ggtcaccgaa acagacacca gcttgtttgc cagcgaacag gcgtactgcg aaagagcatt
    25081 gggttacctg aaagccagtg gtcaaactat ccagtatgaa accttgcctg ataatacctt
    25141 gtcgctggtg gcaccggagg agttacgccg ccgcttcaac cagctaccgc ctgaaattgc
    25201 cccggagaac tggcagctct atttaagtca ggataaaacc gtcatcaccg acgcgattgc
    25261 gcgcgctcgc ggtgagcaac atgcctggcc cgatgtgcag tatctctggc aaatcaaccc
    25321 ggtagtgcag tggctggacg ataaaatctc ttcggctttt ggtcgtcatc aggctccggt
    25381 tatccggctg ccatacctgc ttgaacccga tgaagatcac ttcattcttt ccgggttatt
    25441 cccgaaccgt aaatcacatc cgatggtgaa cccgtggata gtggtgagct ttaaccgtga
    25501 atcgctaatc ggcagtcagc ctttcgctga gtttttacaa cgtcatccgc agttgagtaa
    25561 caagctgact aacagcggcg gtaaagatcg taatcaccaa cgccagcagg atttactgga
    25621 agcggctatt gctcatgccc gggaggtatt catccatgat cggaatgcgt ttgaaacgca
    25681 cattaaccag caactgaatg agcatctgca aaagctggac gttttgcgtg ggcggcagtt
    25741 gagccaactt gagctggatt ttgccgataa caaacagcaa ttgtcagtca agcagagccg
    25801 taaagagcag agacaacgcg aaatcgaaca caattttgac agctatatcg aatggattga
    25861 ggacaccatg acgactgaaa aagaacccta cattcaggta attgctgtta tcaccggagc
    25921 ggagggttaa tcatggcgct ggtcggtatt aataacgaaa acgaatttta ctctaaccac
    25981 tatttgggtg aggttttcac cagtgatatc cgcgatgtgc tggaaccctg gatagcccag
    26041 gaaaatgcag cgcgtgaagc ggagcgtgcc gctcgtgaac agggcaaaga cgtggagccg
    26101 ggataccgcg ctccgtggaa ccagtttaac agtctggcga ctgagttttt ccgcaaactt
    26161 gccgagcacg aaaaacagcg tcagatcccg cagcgtctgg ccgatcaacg taatcgctgg
    26221 cagccattgt taaaggcgct gggctacgaa attacgccac agatccaaat gctggatgac
    26281 gatacaccgc tgccggtact ggcgcgttac aacagcactg acggtagccc gtggctgtgg
    26341 attgttgaag cacacgatca ggaagaagga acgctggatc cgctggcgct ctccttactg
    26401 accgcgcaat tcccggcgga taccgacaaa cataagcacg acagcctgcg caaaaaagcc
    26461 aacggtgaat atcgcagctg gcaggatttg ctctctacgg cggtcttcac ccaaaatgaa
    26521 ccgccgcgtt ttgtgctgct gctcggtaac cgtcagctat tgttgttgga ccgtactaag
    26581 tgggcgcaaa accgtctgct acgttttgat tttgaagaga ttttaagccg tcgtgaaacg
    26641 gatacgctga aagcgactgc ggtgttgtta cataaagatt ctctgctgcc gggcagtggg
    26701 gcaccttatc ttgactcgct ggatgacaat tcgcacaaac atgcgtttgg tgtttcggaa
    26761 gatctgaaat atgccctgcg cgaaagcatt gagttgctgg gcaacgaagc gatgcattat
    26821 ctgatcgacc gtggcctggc aaactataca ggtaatcgtg cggtggaccc ggatgaactg
    26881 agccgcgaat gtctgcgtta catgtaccgc ctgctgttcc tgttctacat tgaagcgcgc
    26941 ccggagctgg gttatgcgcc aatgaccgcc aaaacctatc tgcaaggtta cagcctggaa
    27001 acgttgcgcg atctggagat gatcccgctg accagcgaag aagatcgcaa cgggcgctac
    27061 ttccacgaca gcctgaatat gctgtttaaa ctggtgcgcg aaggctacaa cggcggcgtg
    27121 aaaatgcaga gtgacctgga gagcggcgac cggatcacca tccatagtca tcagttcagc
    27181 gtcccgcgtc tggaaagtca tctgtttgat gccaacaaca cgcgcattct taaccgcgtg
    27241 gtattccgta acgaaaccct gcaacagatt atccaggcga tgtcgttaag ccgcccggcc
    27301 aaagggcgct ttaaccgccg cggacgtatt tcttatcgcc agttgggtat caaccagttg
    27361 ggtgcggtgt atgaggcgct gctctcctat cgcggattct tcgccagcga agatctctac
    27421 gaggtgaaga aagccgggga agagtttaac gagctggaga cgggttactt cgtcagtaag
    27481 gatgagatta gcaaatacca cgaagacgag aaggtctacg agaaagacgg cagtctgcgc
    27541 attcaccgca aaggcagctt tatctaccgt atggccgggc gcgaccgtga gaaatctgcc
    27601 tcttattaca ccccggaagt gctgacccgc tcactggtta aatatgccct gaaagaactg
    27661 tttaaggagc agattgatcc cattagcgat ccgcacgcca aagctgatgc catcttaaac
    27721 ctcaccgtgt gcgaaccggc gatgggcagc gcggcgttcc ttaacgaagc catcaaccag
    27781 ctggcggaag cgtatctgtt ccacaagcag caggcggaag gtcgccgtat tccgcaggat
    27841 cgttacaccc aggagttaca gcgggtgaaa atgtacattg ccgacaacaa cgttttcggc
    27901 gtggacttaa acccggtggc ggtggaactg gcggaagtgt cgctgtggct gaacgccatt
    27961 agtggcgatg cgtttgtacc gtggtttggt taccagctgc actgcggtaa ctcgctggtg
    28021 ggcgcgcgcc gtcaggtgtt caacaagagc gaactgacct acaaaaaagc caaagatccg
    28081 agctggctta acagcgagcc ggtcgaactg gcgatgaaca cgccgcgtga agagacgcag
    28141 attttccact tcctgctgcc cgacggcggt atggctaact acagcgataa aactgttaag
    28201 cagcgttatc cggatgactt caaagcgctg gacagctggc gcaaagagtt tattaaaagc
    28261 tttgccgggc atgagattgc tgatgtgcag cgtatcagcg aaaaggtgga agcactgtgg
    28321 aacacctatc gccagcaact taaagcagaa cgtctgaaaa ccgccgacag ctacccggtg
    28381 tggccggcag aaaacagcga gcagacgcgt tcttcgctga gcagtaaaga tgaaaccttt
    28441 agcggtcgtc ttgaagataa cagcgcctac cagaagctgc gttgggtaat ggactactgg
    28501 tgcgcgctgt ggttctggcc gatcgacaaa gccgatgagc taccggatcg cggcacctgg
    28561 ttgtttgaga ttgaaaccct gcttgacggg attgtaatca cggaaaaagt cactgaagtt
    28621 gcggagcaca ccaccggcga tctgtttgcc gaagaaggcc tgctgcgaga agagtcttcg
    28681 ctgttttctg ttgctggtcg tctgaaaacc gaggtgttgt tccgtcattt gccgcgtctg
    28741 gcgattgtcg atgccctgag aaagcagcac cgtttcttcc actgggatct ggagttctgc
    28801 gacctgtttg ccgagcgcgg cggttttgac ctgatgctcg gaaacccgcc gtggctgaaa
    28861 gtggaatggc aggaagcggg cgtgctgggt gattacgagc cggaatttgt gctgcgtaag
    28921 ctgagcgcct cgaagctggc aacgttgcgt attgatacct ttaaccagat cccggcgctg
    28981 gaagcggcct ggcgcagcga gtatgaaggc tgtgaaggga tgcaaaactt cctgaatgcg
    29041 cagcagaact acccggtact gcgcggggtg cagaccaact tgtataaatg ctttctgccg
    29101 caggcctggc gattaggggc gcagaaaggc gtggcaggtt tcctgcaccc ggaagggatt
    29161 tatgatgacc cgaaaggcgg gcaattacgt gcggcggtat atccgaggct gagggcgcat
    29221 tttcagtttc agaatgagtt aaatttgttt gttgaagttg atcaccatgc gaagtttagc
    29281 agcaatattt attctgctag ccctagcaca gtgggatttg aacatatatc taatttgtat
    29341 gctccgcaaa ctattgatgc atgttttgaa cattctggca gtggggacat tcccggtctc
    29401 aaagacgaga ttgagagcga gggaaaatta aaagttgtat ggaacacatc tggccaccgt
    29461 tctcgattaa taagtatcgc cactcatgag ctagaattat ttgctcgtct atatgacagc
    29521 gaaggaacgc cagcctggca ggcacgtttg ccagccttac atgctaaaca acttgttgct
    29581 gtactggaaa agtttgctaa tcagccgaat agattaggtg atttgcaggg gcagtatttt
    29641 tcaacggtta tgttcgatga aacatatgct cagagggatg ggacaatttt acggcagact
    29701 caattccctc aagattcatc acaatgggta ctgtctggcc ctcatttctt tgttgggacg
    29761 ccgttctaca agactccgcg cgaaaactgt acgcttaaca gcgattatga ctgcctggac
    29821 ttgctaactc tgcctgacga ctatctgccg cgcactaact acattccggc atgtgatgca
    29881 caggagtatg caaaacgtac tccatgcgtt acatggactg aactggctga agatgaaccg
    29941 aagaaggtaa cagattatta tcgcttagct atcagagcca tgttggctca atcgggggaa
    30001 cgtacactaa ttagtgctat ttatccgcca gaaataagtc acatgaacgc agtacgttct
    30061 tactgctata gctcacagaa tctgttactc gaacattcag gtatgtgttt ttctttacct
    30121 tttgatttta tttgtaaatc tactggcaag gcaaacttac atcagatgct tgatggtttc
    30181 tcatacgtat tattcaatcc gagacaaaag gcattattat actgcttagt attatcatta
    30241 aattctgtaa atgatgtata tgctggcctt tggcaatcct gctacacccc agacttcaac
    30301 acccagcgtt ggagccgcga tctcccgcag ctcccccagg atttcttcgc caaactgacc
    30361 ccagagtggc agcgtaactg cgctttacgc tctgactaca gtcgtcgtca ggcgctggtg
    30421 gaaatcgacg tattggtggc gcaggcgctg gggttaactc tcgaagagct gcttaccatt
    30481 tatcgcgttc agttcccggt gatgcgccag tacgaagcgg atacctggta cgatcaaaac
    30541 ggtcgcatta tctttacccc aagcaaaggg ctggtgggcg ttggcttgcc tcgcaccgcg
    30601 cgtaaagctg acctgaaaaa cggctttgtc tttaacgtcg acagcccgga gtggaccggc
    30661 ggtgactgca ccgatcaagc tatcggttgg gatgatgtca aacatcttaa aaccggtacc
    30721 gtcagcgtca cctttgatga ttatacccgc agcgacgaag gtgagcgccg taccgtcacc
    30781 tggcaggctc cgtttatcaa gccagatcgc gaagatgact acaaagtggc ctgggcgttc
    30841 tttgcacaag ataaggagag cgcctgatgt tgccgtccgt tgtcagtcgg caggtcgccg
    30901 acagcgttgc cgccttttta cgggcggcgt tcccgctaaa cagcccgctg tttaacggtg
    30961 agaataacga taatgtctca atgctggagc aatttctggc ccagccggaa acgctgctga
    31021 aaggaccata cctttccgcc cagttgccgt ttcgtaaaag tgacttaccg ctgaattttt
    31081 tccctaatct gacgctgccg ttcccgccgc acgcgcatca ggcccgggca tttcagcgtc
    31141 tgggaagcga cgcgccacag ccgacgctgg tcgcaaccgg taccggctcc ggtaaaacag
    31201 aatgttttat gttcccgctg ctcaaccact gcgcgggcgc gtcacaagcc ggggttaaag
    31261 cgattatcat ctacccgatg aacgccctgg caaccgatca ggccagccgt ttcgccaaaa
    31321 ccatcgccag cgatccacaa ctgcatggca aggtgaccgt cggcctgttt gtcggcgaca
    31381 gcgaaatcga acccagcaaa aaaatgtcgg cagaaaaggt cattacctgt aagcacacgc
    31441 tgcgcgaaag cccgccggac atcctgttga ccaactacaa aatgctcgac tacctgctga
    31501 tgcgccctgg cgatcagccg ctctggcgtt acaaccagcc cggttcattg cgttacctgg
    31561 tcgtggatga attgcatacg tttgatggcg cgcaagggtc ggatctggcc tgtctggtgc
    31621 gacgtttgaa gcatcacata ggtgtggata accaacgatt cgcctgcgtc gggacatcag
    31681 ctaccgtcgg cgacgaactg gggcaactgc tcgattacgc aaaaacaatc tttgaccagc
    31741 cgtttaccga tgacgccgtt attcgcgaag atcgctacac ggcagcggag tacttgcaga
    31801 attacgccat tgcgtacagt cagtatccgg ggccggaggt cgcttcggcc ctggacccgc
    31861 agagttacgc taccccggta gcgtatctta acgggcaaat ccctttgtgg ttccccgaca
    31921 gcgacttgca actcccggac gatctggaga gcgacgacgg gcgtgaaaag cgtattgcgc
    31981 tggggtcact gttacgccgc cacagcgtga tgcatgtctt actccatgat ctgcaaggcg
    32041 ggatcctctc tgaacgagag tgtctggaaa atctccaggt catgctggca gaaagcgccg
    32101 accatgcccg acgcgtgctg caaagcattc tggcactgat cgcgcaggca cgtctcgaag
    32161 taccggaaac ggaacacgat cggcaaaaac gtctccaggc gaagaaagcg cgtccggtgc
    32221 tgccgtttgt gcaactgcgc tcccagttgt ggcttcgtga gatgcgtcgt ctggtcgcca
    32281 gcgtggcgaa aacgccccga ctggtgtttg ccgatgatgt ggcggtggaa gatcgcgaac
    32341 actatttgcc ggttatccac tgccgtgact gccacgcaac tggctgggcc agcctgcgtc
    32401 atgggcaaag cgttcagttg gaaacagacc tcgacggcat ttatcgccag ttctttgaaa
    32461 aagggcgctc catcgtcctt gctttcccgg ataacaacga aaaagcggtc agtggcgcac
    32521 accagaaact gtgtcctacc tgtctgaaac tgaaccgaca gtccagcgtc cagtgtggac
    32581 attgcggtca tacggaactg ttgcaggtac tgatcccgga tatgctcaaa gagcgtaagg
    32641 acgagctgga gtttgctaat gaatgcccgt actgcaacag caaacagggg atttcgattc
    32701 tggggtcacg ggctgccagt ctgtcggcgg tgatgattca ccagctttat ggcagccagt
    32761 tcaacgaaca taaaaagctg attacgtttt ctgactcggt acaagacgcc gcgcatcgtg
    32821 ccagcttctt tactgcccgt acctggccgc tgatgattcg tgggcaaatc gccagtgttc
    32881 ttagcgaaaa agagacatta ccgctggatg cgttcgcggc gcgtgtttgc caacaaacgc
    32941 gtgaacgttc accggacgat gctcattttg cagcaaacgt catcgcacca aatatgcagt
    33001 ggctgaatga ttatcagacg ctgaaaaatg aaggcaaact ggcggagaac tccgatctgc
    33061 cggagctggt ttccctgcgc ctcgactggg aggtgtacag cgaatttacc ctgcgcgcgc
    33121 gtatcggacg tacgctggaa gccaccggtt atgccgttgc cggagtggat aaagatcgct
    33181 tcaatccggc aattaccgcc ctgcatcaac agctttgtga agagctgggt gatgagatgt
    33241 ctggtgtgag cgtcgagcag gtagcgcacc tggcgctggg ggttatctgg tatcaacgcc
    33301 aggccggggc cgttatgcat ccggcgttcg aaagttatcg ccgtgatggc acgaccttta
    33361 tgatgacgca aaaagagcgt acctcgcgct atatgccttc tatcggtccg aaaacgcgta
    33421 aaccggctta tgcgtcgttt gaaaaaatta atggtttcca tcgcctgatt ggcgcgaagt
    33481 ctaaccccag ttggtatcag cattggatca atcgcacgct gagtaacggc aataatctgt
    33541 ttatcagtag cgttgcggaa acagtgttac gacgcctgtt tacggcgttg aaaatagccg
    33601 gagtagtaaa agattttgac accaaagggc gtgaagcctg gggactggtg ccttcggcgc
    33661 tggtggtgag ccgtgacgtc gttcagctta attgctcatg ttgccgtgag gttgtccggg
    33721 ctccggccga tcaaggctgg cagtggcgta atgcgccttg tctctcttta cgttgtgaag
    33781 gtcgttacca ggaagtcgac aatacggcga cctggcgttg ggagaacatg gacatcgcgc
    33841 gcgtacaggg cgcagaacac acggggctgc ttagccgtga agatcgcgaa gtcaccgaaa
    33901 aatcgttcta ccgcggcaac caaccgtgga atatcaacct gttgtcggca acaccgacgc
    33961 tggagatggg tattgacgtt ggggatcttt ccaccgtttt actctgttct gtcccacctg
    34021 ctcaggccaa ctacttgcag cgtattggtc gcgccggtcg taaagatggt aacgcgttaa
    34081 acattaccgt ggcagaaggg aacccgcacg atcagttttt ctttgaagaa ccactggaga
    34141 tgatgcaggg ccaggtgcag gctcctggcg tcttccttaa tgcaacggct attctcgaac
    34201 gccagctggc agctttttgt atggataact gggtgaaaac cggtgtacca gaatccgcta
    34261 tctgtaaaaa cgtcaaacag atgctggatg agctggagtt cgggcgtaaa tcgggttttc
    34321 cgtataactt actgcgatat atcgaacagc atcatgcgga gattgctgcg caatttacgg
    34381 caatcttccc cgatcttgcc gcagaaactc gccaacagct gttgtcttat cttcagggcg
    34441 cgccaggaca acgctcgctg gtgcagcgta tcgaagaagc gttaaaactg ctggtggaag
    34501 atcgcaaatc tttgcgctca cgcatcgata aactcaagcg cagtatcgat aaactggaga
    34561 acgcgccgcg cgatcaaaac tttgatagcg atatgcgcga gttaacttcg gaacggcagg
    34621 cgctgatggc gctggtcaat caaatcaaca ataagcaaac gctgaatttc ctcacggatg
    34681 aaggtctgct gcctaactac gccttcccgg aagccggtat tacgttacgt tctgtgctct
    34741 ggcggcgtaa agaaggcggg gaagcacgtg aatatcaaaa caccactttc gaatatgaac
    34801 gcccggcatc aacggcgctg gctgagctgg ctccgctgaa taatttctat gccggtgggc
    34861 ataaagttga aattgagcag atagacctga aggtctctga gccggagaac tggcgtatct
    34921 gtagccactg taactacagc gaaaatatcg accagacggg cgaccagcat aaatattgcc
    34981 cgaaatgtgg cacaccgggg tgggccgatg ccggacaaaa aaccacgctg ctgaaacttc
    35041 gccaggttta tgcccgcgcc agcgcacgtg atagccagat cagcgacgag agcgacagcc
    35101 gtgaacctgc tttcttccag cgtcagctat tggttagttt cgaaaaagaa gatgtatcgg
    35161 cggcatacgc gattgaggaa ggcgaagttc cgtttggttt tgaattttta agcaaagtca
    35221 ccctgcggga tatcaacttc ggcaaaatgg cggatgatgc caatgagttg atgattgccg
    35281 gagaagcgaa gaagcgaacc gggtttaaag tttgcctcgg gtgcggcatg gtgcagcgtc
    35341 ctcgcgatca tgaaccacgt catgatttga gctgcaaata tcgcacggaa ccggaaaagg
    35401 cgaagttcga agattatctc tacctctatc gccagctgga gtcagaagcg ttgcgtattc
    35461 tgctcccggt aagcagttac agtaatgacc gtgtcgtgga ggcttctctt ggggcggcga
    35521 tccagctcgg tcttaaacat tacttcaagg ggaatgtcga tcacctgaaa ggcgttgtgt
    35581 accgcgagcc ggaaaatgaa ggcgaatcct ggcggcagta tctggtgatt tacgataccg
    35641 tcccgggggg gactggttct cttaaagaac tgatgcgcac tcccgacaat ttgctgaaat
    35701 tacttgaact ggcctatcag gcaatagtgg aatgcagttg caacaatgat acgcataaag
    35761 atggttgtta tcgttgcgtt tacgcttacc gtgaccgtgg gcgcatgaag tatgtctccc
    35821 gcgatcaggc gcgtctcttg ctggcgaaaa ttttgcaggc aagagattcg atacgggtaa
    35881 ttgattccat caaaaacatt tccctcgaag ccatgatggg cagtgagctg gaaaaacggt
    35941 ttattagctg cctgcaagaa aataaaaacc tgattgtcag tcgcagttac gcgcatcagg
    36001 gcgctgggtg gattattaac acccgcagcg aaccgatgat gagctggcat cttaaagctc
    36061 aggtggattt aggcgctaaa gaggcggtgg ggatcccttg tcgcccggat tacgtgctat
    36121 atccgcttat gcagtctgat gccattaaac cggtggctat tttccttgat ggctttgcgt
    36181 tccacaaaaa tagcgtcgca gaggatgtgc aaaaacgcca ggcgattcgg gatagcggta
    36241 atttctgggt gtggacagtc acgtgggccg atctcaaaga gccagggctt aaacatgttc
    36301 aggatgtcct gggacttggg cataacccgg atatgaaaca gcctaagttt tataacctgt
    36361 ttcatgatac caactttgcg gcccttgaag cgtcgttccg ggaacgcaac agttttgcac
    36421 tgctgctgga ttatctcgcc gatccgggcg gaagaacgca gttatggcag agaatggctg
    36481 ctgcacatgc ctgggtgtgg ttggatgtga agaagtcaca ggacgcggga acgaaacaaa
    36541 agtatgccta tgaaatgcag gaaaacgcgc cagcttggcg gctagcagag cttttgccag
    36601 acgaaccgtt tgtctttggt ggtttactcg atagctgtaa ctcatcgcag caatttattg
    36661 agctggcttc ggtgttacca caacaagcca ttaaaccgac gaccagcgtc gcacaaatgc
    36721 gcagctggct gcgtttgcat atctgttttg acgatcgcta tacgcaggat gacggttatg
    36781 aagcgggact caatggcttc tggcgactgg taaacctgtt acagttcctg ccggatatga
    36841 cctttaccag ccgtaaagcc gtgcatttac cgcaggaaag cgttgacact aaagtttcgg
    36901 caacgccaga ggcagtacca gagactgatg agagttgggc tgagattatt gagtttggct
    36961 tattaagcgc tgaagagatc gcgttgcttc agtctcattc tctctctgcg ccgactctcg
    37021 gctacgaatt gcaaaatgat gaaggtgaaa ttattgcgga agccgatctc gcctggccat
    37081 cacaaaaatg ggcattgatt attgataatc aagaatttat tccattattc acaaccaggg
    37141 gttggcacgt tgcattcggg ccaattgacg aaaatacatt gcaacatctg tccggaggtg
    37201 acaaatgacg gtgcaaacgc ccaaggtggc actttctgat ggttttctgg gcgcattcgc
    37261 gcggatccca aaggcacagc aaaaaaaggt gcaggaattc atctccaaat tccgtcagga
    37321 cccgaccagt aatggactta actatgaaaa gatccacgat gcgcgcagta aaaatgtcca
    37381 ttcggtgcga atcgatcaaa cttatcgcgg cattgttctg aaacctgaac agggtgccct
    37441 gtatatgctg atgtgggtgg ataaacacga cgaagcatat gactgggcgc ggcggcatga
    37501 ttgttcaatt cacccggtta ccggcgcgat tcaggtcatt gatattagct atatcaaacc
    37561 ggcagcggaa acagtggttg ataaaccgaa actcttcgct gcttacagcg cggaacagat
    37621 tctggctctg ggtgtgccgc ctgtctttat tgatcaggtg atggcgctta ccgatgaagc
    37681 cgggcttaat caacttgaaa gcataatgcc agcagaagcc tgggaacctc tgcactggct
    37741 ggcagaaggg ctggattacc aggaagtgct ggaagagttt aacgatcatc gtgatgagcc
    37801 tgttgatacc aacgacttta tggaggcgat tgaacgaagt aaacgtcgct tccatgtcgt
    37861 tgaaaatgaa caggagctgt tgcagatgct taatgcgcct ttagaaaaat ggcgtgtatt
    37921 tctgcatccg agccagcgaa aattggtgga gtcgcccgct aatggtccgg tacgagtact
    37981 gggcggtgca ggtacaggga aaacggttgt tgcgatgcac cgtgcgcggt ggttatctca
    38041 gcgtctggca gataaacccg gtaagaaagt acttttcacg acctttacgc gtaacctggc
    38101 ggcagatatt cgcgctaatc tccagcggtt gtgtacgcga gaagagatgg cgcgtattga
    38161 cgtcgttaac attgatgcct ggatgagcga ccaactaaaa cgccacggtt atgatttccg
    38221 cgtggtatac gacagcgatg aagggcgacg caaatgctgg aactatgcct tgcagcaggc
    38281 acctgccgaa ctggggctac ctgataattt ctatgctgaa gagtggcaac gcgttgtcca
    38341 gccaaatgcg gtttacaccc gggaggaata cctcaaagtc agccgggttg gacgtggtac
    38401 agcgttatcc cgtattcaac gggcgaaaat ctggccagtc tttgaagaat tcagagcaca
    38461 aatggcgcgt gccaaactgc gcgaaatggg cgatgccatg catgaggcta ttgttctgtt
    38521 taaagaaaag caggtccaac taccttatag cagtattgtg gtcgatgaag ctcaagatat
    38581 cggtgccccg gcatttactc ttattcgctg cctggttccc gagtcgccca acgatctgtt
    38641 tattgtcggg gatgggcatc agcgtattta tcgcaataag gtcgttcttg gtcagtgtgg
    38701 tattaacgtc cgtggtcgtc gcagtaaaaa attgaagatt aactatcgga ccacagaaga
    38761 gacgcgtcaa tttgctgttg ggctgttaac tggtgtgaag gttgataacc tcgatggcga
    38821 agcggatacc agtaatgatt atttgtcatt actgcacggg gaaaagccca tgattacgca
    38881 tgcggcagat ttcaaagaag aggctgcaac gctcgtcaag caaattcagg cattgctggc
    38941 gaatcaggtt cgttctcagg atatctgcat tactgcgcgt actaaacatg cttgtgaccg
    39001 ctacgcatca gcactgaatg acgccggaat tgagacattt aatttaggca atgatagcgg
    39061 tgacagcgat gcacgccctg gcgtgcgggt tgcaacgatg caccgtatta aagggcttga
    39121 gttccagtat gtgttcctgg tggggattaa cgaaggcgtt gtgccggaaa taaaagcatt
    39181 agcatccgat gatcccgttg aacagcgtga tgcattattt aatgagcgcg cgttactgca
    39241 tgtggctgca acccgtgccg taaaagggct gtttgtttca tcatctggtg tccctagtcc
    39301 gctattagtg gctgactgat aatatgtcca aaacaggcca ggagccgaaa cgctcctggt
    39361 ctatataacc gttctggcaa ctggcgatat ttatattgct tagctcccaa cagccagaca
    39421 atatcaactt agaaattata ttcgcttatg cggtttgcat attgcacagt acaacctgtt
    39481 ttctctatat ctaacgtaac gtcaggatgt ggatgatttt tggatggggt gacacactgg
    39541 acaaatatag ctggcacgaa aaggctggca tttccagctg accaatagtc tctggaacca
    39601 tgatgatgat aaaataaata tcttaagttt taaacgtatg atgccaggat taccagaatt
    39661 ataaacgaat atattagttc ttttctattt gttcaggagg ggtataaaaa ttgcaattgt
    39721 taatagtatc gtcaggacga taaaaagata atcatgacaa aggcaattga actaaaatga
    39781 ttgacatttt acaggtggtt tgtcagtgat ttcttgttat tcataattta ttttccgtaa
    39841 aaaatagtaa ggtgatcacc tgaataaagg taatctcata ttttgatgag tatagtgatt
    39901 ttttatttct ctggtgagga atgaaggaaa tatcaatggt aaggaacgtc agagaatcag
    39961 ggaaaattaa ggtccctcag aaacacaatg ccgttcactt aagcggggcg gcactgtgtt
    40021 tcacaggata acgggttttt gatatcttaa ccgccctcgc ccttgatggt cgggggcgtt
    40081 ttgttatgaa caccacttcc agagcccccg cagatgctcc agtcgtttcg ggatagtccc
    40141 cggtgacgcc gtgtttccca gcactatcat ttctgtcacg ataaactgga acgcgaactt
    40201 aaagctgatc tctgacgccg cttttgttgc ctcacgccgt atcgctccgc cggtaggcgc
    40261 ggtcatggca ttcagtagaa tgtgattccc agattcatta acgccaccag acgcatgacg
    40321 gggtaggcgc tctgccgctc agtggatgtg ttggcagagc cataatattc acgcagctcg
    40381 ggttcatcag gtgtcctgaa ctgtgcgcca tcaatggcaa aaagttgcag gccgtgccag
    40441 tcatccttca ggtaacgttc cgcgccccat gtctgtgtgg tctggcggaa gagccattcc
    40501 accggggcgg cccccacgcg ctgacgcgcc tgggtgacag cgctgcgggc gccagcaggt
    40561 tcatccccgc ttcgccatcc acgctcaggt taagacgacg aacggtcgca tgagcagaaa
    40621 gcgtcaggaa gtgctgtatc cactcggtgg gaaggtgctc tgcaaacagt tgcgtagagg
    40681 gtggtggcat aagcggatgg tcactgaaag tggtataaga aaatggctcc ttgttgtgga
    40741 agtcgttata gtgccgcacg ccggaggagt taaaagcgct gatggatgcg aataaatcaa
    40801 attaatgaac gctcaccttt cgcgccaaaa ctgcaaaatt gcttctacaa acctgtcaga
    40861 aataatcccc cgacgggctg ctgagctgaa atgactgctt cgcagtgccg tcgtccagtt
    40921 ctccggtgac cggtacgctg aaccgtaata tcccgtgctt aaatcgtccg gatcttcatc
    40981 aggcgcgttc gtcagccctc ttgcaccttg ttgctggaag tcgacgaaaa taatattagc
    41041 taaaatatta ttttgataat tgccataaat agcttcatac gcatgaggga aattttcttt
    41101 ccagtaccag gtggtatcgc cgcaaaacca cggcgcgtca gtaatattat taagctgaga
    41161 atggtattgt tttagatccc tacgaaaggc ttcaaccata tgattaaagt gttgaggatg
    41221 tgacgcgtag tcactggtca ttaagtcaaa ttcgccttgc atccagcata cgccgaggaa
    41281 tttgttctgc ggatttttta ccagtgcggc tcgcgttctg ctgactaaat cctggtatag
    41341 cggagtatcc gttccccaac gacaagcatc atggctggct ccgtgccgtt ctgaatatgt
    41401 cccctcgctg cctgcggtaa aagccgatcc gccacggcaa cacggaacga tgagaatccc
    41461 tgcattatca ggaatgaagg gcagtaattt ccgtgcaata tgcagtgcct ggccaacggt
    41521 gccgtactgt gtttgatgat tcgttgccag aggatggtga taactctgca tatcctgaac
    41581 atcgtgtggg cagtgagtca gtggaataat gtcgttaaag tgacatgacg ggcctccggg
    41641 atgcgtatgc gcaaatctcg ctaattgttt aattctggga tgaggcgcat cttccctgtc
    41701 cggtaatggc agtccttcgc catacgccat ggcattagac tgaccagcaa cagtaagaac
    41761 atagtaataa tcaggcgata ttattgcgtt catataaatt tcgcgtaatt gaaatgacaa
    41821 attgatagca gggccccttt accggggccc tgaagcgtta gttttgtact gtgactttat
    41881 tatccttcac agaaatcaaa actgaatccg tcaccgcttt gccgcctgca gtttcaccgc
    41941 caataatcaa tagactatta ttccagggca atgatactcc gtaggcccga ccttgcgata
    42001 attcacccga tttatcccat ttcccgttat gccaaagatg aatatcagtg ctatatgatt
    42061 ttttcaggcc ttcatgcgca tagttcttac cgttttggta attttctcgt gaacctttga
    42121 atccggcccc tccggcaaat ataagagaat cattgcttat ccccgcaaaa ccgccagcga
    42181 cgccatctgg tgatgagacg ggagcaagcc tattccattt taaattatta ccggtgaaat
    42241 caagttcaaa tacggcatcc gttcgcaatc cgggtttggc ttcgccatta ataagccagg
    42301 ttttatcacc tttattcaca accgccgcac cagccgtccc gtaccaggga gattcgccag
    42361 cgtaactcca ttgctgtgta gagggatcaa aagacaacag aaacttatta aagaaataat
    42421 cttctgcttt tttgtcaaaa tagtgtgcat tgattttatc tacagcggtt gaatcttttc
    42481 cagcttcgtt gagatcttca aaatagccat tgaagatatt ctggttaaca ccgccagtaa
    42541 cataagcctt gccgttgtgt acaaaagtca catggcccgc catgcccatc ggcgcgtgcg
    42601 atatcaattt aacccagcta ttggttttgg ggttgtattt gtgtacgtca ttaaatacct
    42661 gagtcaagcc ctcgctgttt ttgccaatgc cgccaaacac atacagattg ccatcaataa
    42721 atgccgaggt tgcttgatct cttggtccac cagggaatgc agctaacgct gtccattttt
    42781 tatctttggc ctgtgtctcc agcttgtacc atgccgtacc tgcgctacct aaaccaatgt
    42841 agacagtgtc gttatcaatt gctccggtac cacttttaaa tggtacagga gtttccggta
    42901 atacagacgc gtttgcggca aatgaagcca ttaggatagc aagcgccgtt attgttttat
    42961 tcattgtgac tgtctcctgt ctactacagt ttaaatgaca caccaatgcg ataactgttt
    43021 tccgataaat tatctctgcc gttgtaaaca ccctgacggt ccaggtagtc atattctatg
    43081 tatggcgtaa tatcgggcgt catatggtat tgtagaacaa atgcattttc cgtcgcccat
    43141 ttcttatggt ttgcatagcg ataatcgttc tgtttgctgt atagcgtcgt ttgccatgcg
    43201 aaggtgaaat cactattaat atggtaagtg acatatccat cccaacgatg aacgttatca
    43261 cgagacatat caccggataa gtcttgttgt cggtaagctt tccagtcgta acgatagcga
    43321 atgccaaaat taagatcttt tgtcgcgtcc caggacagtt ttacgtaggg tccgtagcgt
    43381 gtgccgttgc tgctaaaatg cgttaacatt cccgggcgca ccgtccattg atcatcaagt
    43441 ttaatcgcgt aattaacttc aacctgaaca tcattgagtg cggcattttc ctttttatta
    43501 tcatgaatgg tattccaggt attactttcc atgcttgccc accatccatt ttgccatccc
    43561 tcactgactt tgagtcgagt ctcataggcg tggcttccac tacgatatcc accacgtacg
    43621 tccagtgtcg cagcctgaga aattaatggt gacgaaaagc acagtaataa tacgccagaa
    43681 agtattttag cctttttcat aaatttcact catttgtagg atacagaaag caatacaaag
    43741 cccgcataaa caattagcat ttatgttgtg taatattttt ttgccaggct tatagtgtct
    43801 ttggcaaccg gtagctgtat tttatatttt ttgtataatg tctcctgtga aaaatctctt
    43861 tccacattat ttaaataaac agagagccag attaaatacc tgagtataaa atctattctg
    43921 atgtttaatt gatttgaatg ttcgtaagct atatcactta ctcaattcat tttacccaga
    43981 gtcataaaat agagtgtctg ttgatctcat tttcatattc ctttcaggct gaagtgcgtt
    44041 ttcatcaccc gatgagaaag aactgaagtg atgtttgcca tagattgcaa gatgccctgt
    44101 ctcattgtct atcgtggtgc acctttatac ctgttatacc agatcgaaaa tcacgcaatc
    44161 catacaacaa aaccagattt gcaattcgtg tcacaaaata tgtcgatctt tttctaagaa
    44221 gaagatgcca tgtgaagcca gacgaacact tgcggtgg
//
LOCUS       DAZGXS010000023        42359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGXS010000023 DAZGXS010000000
VERSION     DAZGXS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(104..779)
                     /locus_tag="ACHKG5_001409"
                     /pseudo
     CDS             complement(104..779)
                     /locus_tag="ACHKG5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(924..1532)
                     /gene="yiiX"
                     /locus_tag="ACHKG5_001410"
     CDS             complement(924..1532)
                     /gene="yiiX"
                     /locus_tag="ACHKG5_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0867541.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1592..1909)
                     /gene="metJ"
                     /locus_tag="ACHKG5_001411"
     CDS             complement(1592..1909)
                     /gene="metJ"
                     /locus_tag="ACHKG5_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0867542.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2186..3346
                     /gene="metB"
                     /locus_tag="ACHKG5_001412"
     CDS             2186..3346
                     /gene="metB"
                     /locus_tag="ACHKG5_001412"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0867543.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3349..5781
                     /gene="metL"
                     /locus_tag="ACHKG5_001413"
     CDS             3349..5781
                     /gene="metL"
                     /locus_tag="ACHKG5_001413"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0867544.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5920..6054)
                     /locus_tag="ACHKG5_001414"
     CDS             complement(5920..6054)
                     /locus_tag="ACHKG5_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867545.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6130..7020
                     /gene="metF"
                     /locus_tag="ACHKG5_001415"
     CDS             6130..7020
                     /gene="metF"
                     /locus_tag="ACHKG5_001415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0867546.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7349..9529
                     /gene="katG"
                     /locus_tag="ACHKG5_001416"
     CDS             7349..9529
                     /gene="katG"
                     /locus_tag="ACHKG5_001416"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0867547.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9623..10528
                     /gene="yijE"
                     /locus_tag="ACHKG5_001417"
     CDS             9623..10528
                     /gene="yijE"
                     /locus_tag="ACHKG5_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0867548.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10555..11172)
                     /gene="yijF"
                     /locus_tag="ACHKG5_001418"
     CDS             complement(10555..11172)
                     /gene="yijF"
                     /locus_tag="ACHKG5_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0867549.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11446..12549)
                     /gene="gldA"
                     /locus_tag="ACHKG5_001419"
     CDS             complement(11446..12549)
                     /gene="gldA"
                     /locus_tag="ACHKG5_001419"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0867550.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12560..13222)
                     /gene="fsa"
                     /locus_tag="ACHKG5_001420"
     CDS             complement(12560..13222)
                     /gene="fsa"
                     /locus_tag="ACHKG5_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0867551.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13234..15735)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_001421"
     CDS             complement(13234..15735)
                     /gene="ptsP"
                     /locus_tag="ACHKG5_001421"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0867552.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16044..17123
                     /gene="frwC"
                     /locus_tag="ACHKG5_001422"
     CDS             16044..17123
                     /gene="frwC"
                     /locus_tag="ACHKG5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0867553.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17138..17458
                     /gene="frwB"
                     /locus_tag="ACHKG5_001423"
     CDS             17138..17458
                     /gene="frwB"
                     /locus_tag="ACHKG5_001423"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0867554.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17509..19806
                     /gene="pflD"
                     /locus_tag="ACHKG5_001424"
     CDS             17509..19806
                     /gene="pflD"
                     /locus_tag="ACHKG5_001424"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0867555.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19772..20650
                     /gene="pflC"
                     /locus_tag="ACHKG5_001425"
     CDS             19772..20650
                     /gene="pflC"
                     /locus_tag="ACHKG5_001425"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0867556.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20652..20993
                     /gene="frwD"
                     /locus_tag="ACHKG5_001426"
     CDS             20652..20993
                     /gene="frwD"
                     /locus_tag="ACHKG5_001426"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0867557.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20980..21831)
                     /gene="yijO"
                     /locus_tag="ACHKG5_001427"
     CDS             complement(20980..21831)
                     /gene="yijO"
                     /locus_tag="ACHKG5_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0867558.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22046..23779)
                     /gene="eptC"
                     /locus_tag="ACHKG5_001428"
     CDS             complement(22046..23779)
                     /gene="eptC"
                     /locus_tag="ACHKG5_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0867559.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23962..26613)
                     /gene="ppc"
                     /locus_tag="ACHKG5_001429"
     CDS             complement(23962..26613)
                     /gene="ppc"
                     /locus_tag="ACHKG5_001429"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0867560.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26965..28116)
                     /gene="argE"
                     /locus_tag="ACHKG5_001430"
     CDS             complement(26965..28116)
                     /gene="argE"
                     /locus_tag="ACHKG5_001430"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0867561.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28270..29274
                     /gene="argC"
                     /locus_tag="ACHKG5_001431"
     CDS             28270..29274
                     /gene="argC"
                     /locus_tag="ACHKG5_001431"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0867562.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29282..30058
                     /gene="argB"
                     /locus_tag="ACHKG5_001432"
     CDS             29282..30058
                     /gene="argB"
                     /locus_tag="ACHKG5_001432"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0867563.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30119..31492
                     /gene="argH"
                     /locus_tag="ACHKG5_001433"
     CDS             30119..31492
                     /gene="argH"
                     /locus_tag="ACHKG5_001433"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0867564.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31759..32676
                     /gene="oxyR"
                     /locus_tag="ACHKG5_001434"
     CDS             31759..32676
                     /gene="oxyR"
                     /locus_tag="ACHKG5_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0867565.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32659..34059)
                     /gene="sthA"
                     /locus_tag="ACHKG5_001435"
     CDS             complement(32659..34059)
                     /gene="sthA"
                     /locus_tag="ACHKG5_001435"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0867566.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34389..35555
                     /locus_tag="ACHKG5_001436"
     CDS             34389..35555
                     /locus_tag="ACHKG5_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFP0867567.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            35598..36914
                     /locus_tag="ACHKG5_001437"
     CDS             35598..36914
                     /locus_tag="ACHKG5_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFP0867568.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            37018..37668
                     /gene="fabR"
                     /locus_tag="ACHKG5_001438"
     CDS             37018..37668
                     /gene="fabR"
                     /locus_tag="ACHKG5_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0867569.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            37668..38027
                     /gene="yijD"
                     /locus_tag="ACHKG5_001439"
     CDS             37668..38027
                     /gene="yijD"
                     /locus_tag="ACHKG5_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0867570.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38067..39167)
                     /gene="trmA"
                     /locus_tag="ACHKG5_001440"
     CDS             complement(38067..39167)
                     /gene="trmA"
                     /locus_tag="ACHKG5_001440"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0867571.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39281..39471
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39536..41380
                     /gene="btuB"
                     /locus_tag="ACHKG5_001441"
     CDS             39536..41380
                     /gene="btuB"
                     /locus_tag="ACHKG5_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0867572.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41325..42182
                     /gene="murI"
                     /locus_tag="ACHKG5_001442"
     CDS             41325..42182
                     /gene="murI"
                     /locus_tag="ACHKG5_001442"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0867573.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9611 a        10560 c        11748 g        10440 t
ORIGIN      
        1 agagcagaat gaggtaaaaa tctttggctg tgtattcagt ctggtaatca tccaaggaaa
       61 gctcatcaga agggtgatga ctccaattag tagaacatat attttcgata aaagcaccga
      121 tgactttttc atgaagttct acagaactcg agaaacagat ggttctgcgg gccagacgtt
      181 tgattcgggt tctcagggtc agattgttac gctcaatgtg ctgagtaaaa atcttaccag
      241 tcagatgctt ctcctttggt aactctctag cgtaactacc ccattcgtca gtggtcaaca
      301 tgccgatggt aaaagggtaa gcagcgccag caattcacga caggtttcat cagtccgggg
      361 gccaaaagtg taggccagaa caccaccagt tttcgtgttg tacacgtacc agagccagtg
      421 ctgtcgcgct ttactgccga caaagctcca ttgctgacta agtttgcaga tgagtgccac
      481 atcagcatga gcgaccgggg atgacgttat tcttcgtggc gagagttttt taaagttcgg
      541 atgactgtat taatgccgat tttcagcgta cgggcagtat agcgaacccc ggcaccgttg
      601 aaagccattt cagcgatttg gtctttaact cccggtttac ggggctcgta acagtaactg
      661 agctgaaata cccggtgaca gtcacggccg cggaatcgat catgtctttt ggggttctgc
      721 ccgcgacggt aaacctgaac ggactggcaa cggggacaat gaaggttaac tgtgaccatg
      781 aaacatcctt aaaagcgagt attatatcct taattcaact aattggaggc atcaccgttt
      841 accggagcga cagcttgcgg aaaggtcgtg caactacaaa gcaacaaaca gactacggcg
      901 ggaattattt ttttcatcat ccattaccac gaaaacagcg gccattcttt ggcaaccgtg
      961 gtgagttgtg gcgcatcgaa aacggcttgt ggtgagacca ccgtttcctc aagcggaata
     1021 tttttgccgt agcgttcttt gagcttcgcc tggaccagcg ggttgctgag atcaaactct
     1081 ttcaacttct gctgttcgcc cacacgcatt cccagcgcat tctgataaac cttccacacc
     1141 acttccgaac agtactggcg atcgtcgctc catgagaagc tgaaatcgta cggtttgccg
     1201 agataacgtt tcgccgtttg cgccagtttt tgctgttgtt caacactcag tccgccttca
     1261 acgcggcgaa caacgtattt gcccttttca ccatgggcaa tccactgctt gagcggggtg
     1321 tatttcaccg ggccgaccgc ttcaaaaacg tagggctttt tgttacgcat caccagcata
     1381 ccggtgtggc tgtaatcgga atgggtcgcc agttggatcg ctttactttg cgatgagcga
     1441 gagatttgaa agatgatgtc gccagtttgc ggctgccagg caaaggcagg cacagaaacc
     1501 agcaggctga ggatcagtag cctgattttc atcgttaaac gtccttgtat gaaaaaagcg
     1561 gctggttaac tccgcgccgc tcttttccgc tttagtattc ccacgtctcc gggttaatcc
     1621 ccatctcacg catgatctct tttgccgctt ccgggatttc gtcgctgcgc tctttacgca
     1681 gatcggcatc atccggcaaa ggttgcccgg taaaggcatg cagaaacgct tcgcacagca
     1741 gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg acgcgtgcgt tcatcggtga
     1801 ggatttttaa caccttaaga ggaatggaaa ccgtaatctt tttgacttgt tcactcttct
     1861 tgccgtgctc agcgtatggg ctgatatatt cgccgctcca ttcagccatg agatacttaa
     1921 tcctcttcgt caataaattg agaccagacc acacaattta tgtgggtact gaccgtaaac
     1981 ccgcatagtt taccgtacag gcgttaccgt gacatcgtgt aatgcacctg tcggcgtgat
     2041 aatgcatata attttaacgg ctatttggga tttgctcaat ctatacgcaa agaagtttag
     2101 atgtccagat gtattgacgt ccattaacac agtgtttact ctggtgcctg acatttcacc
     2161 gaccaagccc agggaacttc atcacatgac gcgtaaacag gccaccatcg cagtgcgtag
     2221 cgggttaaat gacgacgaac agtatggttg cgttgtccct cccatccatc tttccagcac
     2281 ctataacttt accggattta atgaaccgcg cgcgcatgat tactcgcgtc gcggcaaccc
     2341 aacgcgcgat gtggttcagc gtgcgctggc agaactggaa ggcggtgctg gtgcagtact
     2401 tactaatacc ggcatgtccg cgattcatct ggtaacgacc gtctttttga aacctggcga
     2461 tctgctggtt gcgccgcacg actgctacgg cggtagctat cgcctgttcg acagcctggc
     2521 gaaacgcggt tgctatcgcg tgttgtttgt tgatcaaggc gatgaacagg cattacgggc
     2581 agcgctggca gaaaacccca aactggtact ggtagaaagc ccaagtaatc cattgttacg
     2641 cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa gtcggggcgg tgagcgtggt
     2701 ggataacacc ttcttaagcc cggcattaca aaatccgctg gcattaggtg ccgatctggt
     2761 gttgcattca tgtacgaaat atctgaacgg tcactctgac gtggtggctg gcgtggtgat
     2821 tgctaaagac ccggacgttg tcactgaact tgcctggtgg gcaaacaata ttggcgtgac
     2881 gggcggcgcg tttgacagct atctgctgct acgcgggttg cgaacgctgg tgccgcgtat
     2941 ggagctggcg cagcgcaacg cgaaggcgat tgtgaaatat ctgcaaaccc agccgttggt
     3001 gaaaaaactg tatcacccgt cgctgccgga aaatcagggg catgaaattg ccgcgcgtca
     3061 gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat ggcgatgagc agacgctgcg
     3121 ccgtttcctg ggcgggctgt cgttatttac gctggcggaa tcattagggg gagtggaaag
     3181 tttaatctct cacgccgcaa ccatgacaca tgcaggcatg gcaccagaag cgcgtgctgc
     3241 cgccgggatc tccgagacgc tgctgcgtat ctccaccggt attgaagatg gcgaagattt
     3301 aattgccgac ctggaaaatg gcttccgggc tgcaaacaag gggtaaaaat gagtgtgatt
     3361 gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg gtggcagtag tctggctgat
     3421 gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt actctcagcc tgacgatatg
     3481 atggtggttt ccgccgctgg tagcaccact aaccagttga ttaactggtt gaaactaagc
     3541 cagaccgatc gtctctctgc gcatcaggtt caacaaacgc tgcgtcgcta tcagtgcgat
     3601 ctgattagcg gtctgctacc cgccgaagaa gccgatagcc tcattagcgc ttttgtcagc
     3661 gatcttgagc gcctggcggc gctgctcgac agcggtatta acgacgcagt gtatgcggaa
     3721 gtggtgggcc acggcgaagt gtggtcggcg cgcctgatgt ctgcggtgct taatcaacaa
     3781 gggctgccag cggcctggct tgatgcccgc gagtttttac gcgctgaacg cgctgcgcaa
     3841 ccgcaggttg atgaagggct ttcttacccg ttgctgcaac agctactggt acaacatccg
     3901 ggtaaacgtc tggtggtgac cgggtttatc agccgtaaca acgccggtga aacggtgctg
     3961 ctggggcgta acggttccga ctattccgcg acacaaatcg gtgcgctggc gggtgtttct
     4021 cgtgtaacca tctggagcga cgtcgccggg gtatacagtg ccgatccgcg taaagtgaaa
     4081 gatgcctgtc tgctgccatt gctgcgtctg gatgaagcca gcgaactggc gcgtctggcg
     4141 gctcccgttc ttcacgcccg tactttacag ccagtatccg gtagcgaaat cgacctgcag
     4201 ctgcgctgta gctacacgcc ggatcaaggc tccacgcgca ttgaacgcgt gctggcctcc
     4261 ggtactggtg cgcgtattgt caccagccac gatgatgtct gcttgattga gtttcaggtg
     4321 cccaccagcc aggatttcaa actggcgcat aaagagatcg accagatcct gaaacgtgct
     4381 caggtgcgcc cactggcggt tggcgtgcat aacgatcgcc agttgctgca attttgctac
     4441 acctcagaag tggccgacag tgcgctgaaa atcctcgacg aagcgggatt acctggcgaa
     4501 ctgcgcctgc gtcaggggct ggcgctggtg gcgatggtcg gggcgggtgt cacccgtaac
     4561 ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc agccggtcga gtttacctgg
     4621 cagtccgatg acggcatcag cctggtggca gtactgcgca ccggcccgac cgaaagcctg
     4681 attcagggcc tgcatcagtc cgtcttccgc gcagaaaaac gcatcggcct ggtattgttc
     4741 ggcaaaggca atatcggttc ccgctggctg gaactgttcg cccgcgagca gagcacgctt
     4801 tcggcgcgta ccggctttga atttgtgctg gcaggcgtgg tggacagccg ccgcagtctg
     4861 ttgagttatg acgggctgga cgccagccgc gcgttagcct tcttcaacga tgaagcggtt
     4921 gagcaggatg aagagtcgtt gttcctgtgg atgcgcgccc atccgtatga tgatttagtg
     4981 gtgctggacg ttaccgccag ccagcagctg gctgaccagt atcttgattt cgccagccac
     5041 ggtttccacg tcatcagtgc caacaaactg gcgggagcaa gcgacagcaa taaatatcgc
     5101 cagatccacg acgccttcga aaaaaccggg cgtcactggc tatacaacgc caccgtcggt
     5161 gcgggcttgc cgatcaacca taccgtgcgc gatctgatcg acagcggcga tactattttg
     5221 tcgatcagtg ggatcttctc cggcacgctc tcctggctgt tcctgcaatt cgacggtagc
     5281 gtgccgttta ccgagctggt ggatcaggcg tggcagcagg gcttaaccga gcctgatccg
     5341 cgtgacgacc tctccggcaa agacgtaatg cgcaagctgg tgattctggc gcgtgaagcg
     5401 ggttacaaca ttgaaccgga ccaggtacgt gtggaatcgc tggtgcctgc tcattgcgaa
     5461 ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac ttaacgagca gatggtgcaa
     5521 cggctggaag cggcccgcga aatggggctg gtgctgcgct acgtggcgcg tttcgatgcc
     5581 aacggcaaag cgcgtgtggg cgtggaggcg gtgcgtgaag accatccgct ggcatcactg
     5641 ctaccgtgcg ataacgtctt tgccatcgaa agccgctggt atcgcgacaa cccgctggtg
     5701 atccgtgggc ctggcgcggg gcgcgacgtc accgccgggg cgattcagtc ggatatcaac
     5761 cggctggcac agttgttgta atttcagaaa tttaataatg cccggtactc atgttttcgg
     5821 gtttatggtt tctaatgaaa tatattgaat tatcatagga ttaggccgga tgaagcgttt
     5881 acgacgaatc cggcaagaag caataagtac atggttagtt tatatttgca gtccggtttg
     5941 ctttgcatac cggattttct ttttcttacc atcctgaagt tttttcatct tccctgattt
     6001 ttcctcacca tcattggtca tttttcggtt gacgcccttc ggcttttcct tcatctttac
     6061 atctggacgt ctaaacggat ggatgtgcac aacacaacat ataactacaa gcgattgatg
     6121 aggtaaggta tgaacttttt tcacgccagc cagcgggatg ccctgaatca gagcctggca
     6181 gaagtccagg ggcagattaa cgtttcgttc gagtttttcc cgccgcgtac cagtgaaatg
     6241 gagcagaccc tgtggaactc catcgatcgc cttagcagcc tgaaaccgaa gtttgtatcg
     6301 gtgacctatg gcgcgaactc cggcgagcgc gaccgtacgc acagcattat taaaggcatt
     6361 aaagatcgca ctggtctgga agcggcaccg catcttactt gcattgatgc gacgcccgac
     6421 gagttgcgca ccattgcacg cgactactgg aataacggta ttcgtcatat cgtggcgctg
     6481 cgtggcgatc tgccgccggg aagtggtaag ccagaaatgt atgcttctga cctggtgacg
     6541 ctattaaaag aagtggcaga tttcgatatc tccgtggcgg cgtatccgga agttcacccg
     6601 gaagcaaaaa gcgcccaggc ggatttgctt aacctgaaac gcaaagtcga tgcaggagcc
     6661 aaccgcgcga ttactcagtt cttctttgat gtcgaaagct acctgcgttt tcgtgaccgc
     6721 tgtgtgtcgg cgggcattga tgtggaaatc attccgggca tattgccggt atctaacttt
     6781 aaacaggcga agaaatttgc cgacatgacc aacgtgcgta ttccggcgtg gatggcgcaa
     6841 atgtttgacg ggctggatga cgatgccgaa acccgcaagc tggtgggcgc gaatattgcc
     6901 atggatatgg tgaagatttt aagccgtgaa ggagtgaaag atttccactt ctatacgctt
     6961 aaccgcgctg aaatgagtta cgcgatttgc catacgctgg gggttcgacc tggtttataa
     7021 atagtgtggc ttttatgaaa atcacacagt gatcacaaat tttaaacaga gcacaaaatg
     7081 ctgcctcgaa atgagggtgg gaaaataagg ttatcagcct tgttttctcc ctcattactt
     7141 gaaggatatg aagctaaaac ccttttttat aaagcatttg tccgaattcg gacataatca
     7201 aaaaagctta attacgatca atttgatcta catctcttta accaacaata tgtaagatct
     7261 caactatcgc atccgtggat taattcaatt ataacttctc tctaacgctg tgtatcgtaa
     7321 cggtaacact gtagagggga gcacattgat gagcacgtca gacgatatcc ataacacgac
     7381 agccactggc aaatgcccgt tccatcaggg cggtcacgac cagagcgcgg gggcgggcac
     7441 aaccactcgc gactggtggc caaatcaact ccgtgttgac ctgttaaacc aacattctaa
     7501 tcgttctaac ccgctgggtg aggactttga ctaccgcaaa gaattcagca aattagatta
     7561 ctacggcctg aaaaaagatc tgaaagccct gctgacagaa tctcagccgt ggtggccagc
     7621 cgactggggc agctacgccg gtctgtttat tcgtatggcc tggcacggcg cggggactta
     7681 ccgatcaatc gatggacgcg gtggcgcggg tcgtggtcag caacgttttg caccgctgaa
     7741 ctcctggccg gataacgtaa gcctcgataa agcgcgtcgc ttgttgtggc caatcaaaca
     7801 gaaatatggt cagaaaatct cctgggccga cctgtttatc ctcgcgggta acgtggcgct
     7861 ggaaaactcc ggcttccgta ccttcggttt tggtgccggt cgtgaagacg tctgggaacc
     7921 ggatctggat gttaactggg gtgatgaaaa agcctggctg actcaccgtc atccggaagc
     7981 gctggcgaaa gcaccgctgg gtgcaaccga gatgggtctg atttacgtta acccggaagg
     8041 cccggatcac agcggcgaac cgctttctgc ggcagcagct atccgcgcga ccttcggcaa
     8101 catgggtatg aacgacgaag aaaccgtggc gctgattgcg ggtggtcata cgctgggtaa
     8161 aacccacggt gctggtccga catcaaatgt aggtcctgat ccagaagctg cgccgattga
     8221 agaacaaggt ttaggttggg cgagcactta cggcagcggc gttggcgcag atgccattac
     8281 ctctggtctg gaagtggtct ggacccagac gccgacccag tggagcaact atttcttcga
     8341 gaacctgttc aagtatgagt gggtacagac ccgcagtccg gctggcgcaa tccagttcga
     8401 agcggtagac gcaccggaaa ttatcccgga tccgttcgat ccgtcgaaga aacgtaaacc
     8461 gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct gagttcgaga agatctctcg
     8521 tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt gcccgtgcct ggttcaaact
     8581 gacgcacagg gatatggggc cgaaatctcg ttacatcggg ccggaagtac cgaaagaaga
     8641 tctgatctgg caagatccgc tgccgcagcc gatctacaac ccgaccgagc aggacattat
     8701 cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt agtgagctgg tatcggtggc
     8761 ctgggcttct gcttctacct tccgtggtgg cgacaaacgc ggtggtgcca acggtgcgcg
     8821 tctggcatta atgccgcagc gcgactggga tgtgaacgcc gcagccgttc gtgctctgcc
     8881 tgttctggag aaaattcaga aagagtctgg taaagcctcg ctggcggata tcatcgtgct
     8941 ggcaggtgtg gttggcgttg agaaagccgc aagcgccgct ggtttgagca ttcatgtacc
     9001 gtttgcgccg ggtcgcgttg atgcgcgtca ggatcagact gacattgaga tgttcgaact
     9061 gctggagcca attgctgacg gtttccgtaa ctatcgcgct cgtctggatg tttccaccac
     9121 cgagtcactg ctgattgata aagcccagca actgaccctg accgcgccgg aaatgaccgc
     9181 gctggtgggc gggatgcgtg tactgggtgc caacttcgat ggcagcaaaa acggcgtctt
     9241 cactgaccgc gttggcgtat tgagcaatga cttcttcgtg aacttgctgg atatgcgtta
     9301 cgagtggaaa gcgaccgacg aatcgaaaga gctgttcgaa ggccgtgacc gcgaaaccgg
     9361 cgaagtgaaa tacactgcca gccgtgcgga tctggtattt ggttctaact ctgtcctgcg
     9421 tgcggtggct gaagtttacg ccagcagcga tgctcacgag aagtttgtta aagacttcgt
     9481 ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac ctgctgtaat ctgaccttct
     9541 tcagcgactg cctttcaggc agtcgctgaa gtttctttac cggcgtatag tgtccacagg
     9601 aaaactacac actggatctc tcatgtctgc cgcaggaaag agcaacccac tggcaatcag
     9661 tggcctggtt gtgctcacac ttatctggag ttatagctgg attttcatga agcaagtcac
     9721 cagctacatc ggtgccttcg actttaccgc cttacgctgc attttcggcg ctctcgtttt
     9781 attcatcgtc cttttattac gtggtcgcgg aatgcgcccg acaccgttta aatacacctt
     9841 agccattgcc ctgttacaaa cctgcgggat ggttggcctg gcgcagtggg cgctggtcag
     9901 cggcggagcg gggaaggtgg cgatcctgag ctataccatg ccgttctggg tggtgatttt
     9961 cgccgcggtg tttctcggtg aacgcctgcg acgtgggcaa tatttcgcga ttctgattgc
    10021 cgctttcggc ttatttttgg tgttgcagcc gtggcaactc gatttctctt cgatgaaaag
    10081 tgccatgctg gcaatcctct ccggtgttag ttggggggcg agcgcgattg ttgctaaacg
    10141 tctgtatgcc cgtcatccgc gcgtggattt attgtcgtta acatcctggc agatgctcta
    10201 tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg caacgtgaaa ttgactggca
    10261 gcccactgtg ttctgggctc tggcctacag tgcgattctg gcgacggcgt tggcgtggag
    10321 cttatggctg tttgtgctga aaaacttgcc tgccagtatt gccagcttaa gcacactggc
    10381 cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc ggtgagaatc cgggggccgt
    10441 tgaaggtagc ggtattgtgc tgattgtgct ggcactggcg ctggtgagcc gtaagaaaaa
    10501 agaagccgtt agtgtaaaaa ggatctgaat tttttcttca tgtggggcga tcgcttattt
    10561 aacaaaataa cgataatgcc ccaccatgcg ccagctaaac agcacatctt cttcctgcgc
    10621 acctgcgcca atgttatgta tcaccagcgg cgtaccgtcg cgggcgaagc catctgaaac
    10681 caccccaata tgtgccagcc cgttatctaa tcgccaggag acaatatcgc ccgcttgata
    10741 gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg cgggtaaacc aggtttccag
    10801 attaggcacc cgacggtgat cgatgttgct gtccgggcgc tttaactgcc atttttgcgg
    10861 gtactcggca aaattcttcg ccatatcttc gtgcaccagt ttctgcaaat cgaccttctg
    10921 gctgcgcaat gcacggatca ccacatcgga acataccccg cgttcttgcg ggacatcacc
    10981 gccaggatag gtaagctgca catacgccgg atcgtaaaat agcgtgctgc cgatttgctg
    11041 tctggcaccg tctgcgatgg caaggttggt attggcctgg atttgtacca cggttggtgg
    11101 aatggcggga gattttaagg agtagctggt aaatgccgtt agcaggctga ccagcgccag
    11161 tgaagctttc atctgtttcg tccgtgaaaa taagagtcac agattcgacc ttccgggcta
    11221 acgattgttc tgtagagaaa atgtccagaa gcggataaaa aattcctgaa caacgtggga
    11281 gtttcagatt actggcacag agtgccggat gcggcgtgaa cgtcttatct ggcctaaaaa
    11341 gcacgcaaat tcaatatatt gcagagattg cgtaggcctg ataagcgtag cgcatcaggc
    11401 aattttgcgt tcaaactccc ggacaagccg ggagtttgga gtaggttatt cccactcttg
    11461 caggaaacgc tgaccgtact ggtcggctac cagcagagcg gcgtaaacct gatctggcgt
    11521 cgcgccgcca ggcatgttgt ggatggtttc accttctgca catgccgctt ctgccacaat
    11581 tcgcattttc gccgggacat cttctttaat atccagttga gcgagagtta ttggcaaacc
    11641 taccgcatgg ctaagcgcag ctacggtttc gatttcctca accggcgagt tttccagtac
    11701 cagctgcgtc agtgtaccaa atgccacttt ttcaccgtga taatagtgat gggcatcagg
    11761 aatggcagtc aggccattat gcaccgcgtg cgctgccgcc agaccaccgc tttcaaagcc
    11821 aacaccgctt aaataagtgt tcgcttcaat cacgcgttcc agtgccggag tcactacatg
    11881 ctgttcggcg gcgagcatcg ctttttcgcc ttcttccagc aaggtgttgt agcacagttc
    11941 agccagcgcc agcgccgcct gagtgcactt gccgcccgcc atggtggtcg caccgctacg
    12001 agagcaggca cgcgcttcaa accaggtcgc cagcgcatcg ccgatacccg ccgctaacag
    12061 acgcgcaggt gcgcccgcga cgattttagt atcgacaatc accatattcg ggttatttgg
    12121 cagcagcaga tagcggtcaa attcaccttc gtcagtgtag ataacggaaa gagcgctaca
    12181 aggtgcgtcg gttgaagcaa tggtcggggc aatggcgacc ggaacaccca tgaaatgtgc
    12241 cagtgctttg gctgtatcga gggtttttcc gccaccgata ccgagaattg cgccacactg
    12301 cgcagtctcc gcgatgccac gcagacggtc gatctcattt tgcgaacatt caccgccaaa
    12361 cggcgcaatc tctactacca gtccagcatc tttaaagctt ttctcgacag tggattgagc
    12421 aaaacctaaa acaaatttgt cacccaccac taaccagcgt tctgccagcg gcttcaggta
    12481 ttcgcccaga cgattaatca catcagcgcc ctggatgtat ttacccggtg attgaataat
    12541 gcggtccata attgctcctt tagagatgag tagtgccaaa tgcggcattc cagtcgtgtt
    12601 cgaacttctc tatagctgac tctaccgcag gggtgttgag catttgttgc gctacatcta
    12661 agggcagggt gatggattca catcctgcca gtaaacagtc cagcgcctga cgcggcgttt
    12721 taaagctggc cgccagcacc atgctttctg gtgcgtgcat ttctaacagc gcttgcagct
    12781 cctgaaccgt acgaatgccg tccccgccct gggcatccac gcggttaaca tacggagcaa
    12841 cgtattttgc ccctgccagt gcggctaata acccttgtgc ggcgctatat acagcagtgc
    12901 caagtgtagt aataccctct tttttcagta ttttaattgc tgccagacct tcggaagtca
    12961 ccgggatttt caccacaatg cccggaatgg catcgcgcag acgcttcgct tcttccacca
    13021 tcccctgcgc gtcgcggctc atggtctgag caaacagaat gccctcatca ccaatcgctt
    13081 tttgcagacg cggcagcact tcccatatgg actccttgct ggcagcgata atgctcgggt
    13141 tagttgtcac cccggcaatc gggaatatgt gtgccagacg ttcgacttct gcgacgttag
    13201 cggtgtccag atacagttcc atgatgtttc cttttacagt tccagttcat gttgcagcag
    13261 gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg ttacggaatt cttcgtgcat
    13321 gatgcggcga gcgaggcgcg agaaaatgcg catatgctga tcgcccgcag cgtgtttgtt
    13381 cagggttaac ataatgatga attgcgcttc atcatcgccc cagcgcaccg gcgcttgcag
    13441 acgcgctacg ctgatggtgg attgctcaat gtgttctgat ttgctgtggg gaatggcaaa
    13501 actaaagccc agaccggtag agaaaacggc ctcgcgcgcc cacaagtcgg cttccagttt
    13561 acgcggatag cggcagcggc ccgccagcag caggttatcg gtcatgcctt tgagcacttc
    13621 ttctttgctg cgccagtcgc tttccagtgt gatgcactcg gcggtgacca gcggtgcgtc
    13681 ttgttgggtc atgcggaatt gcgccagcag gtgttccact tccagcgaag tacggcaggc
    13741 cattgcctgg ttgagcaact tgcggcactc acggctatca agttgcgcca tccgcgcttt
    13801 cgccgccgga attgatggtg cgctcatgct gagttcatcc agccctaagc cgaccagcaa
    13861 cggcagcacg gaacctttcg ctcccagctc accgcacaga ccaatccatt tgccctggcg
    13921 atgcaccgct tgcacggcgt aatcgagcgc ccgcaagaat gccggattca ggctgttgta
    13981 gtgacgagta accttagcgt tatcgcgatc cactgccagc agatattgcg tcaggtcatt
    14041 actaccaata ctaaagaaat caatctcttc gcagcattga tcgatgatga acatcaccga
    14101 cggcacttcc agcatgatgc cgagctggat cttctcatca aacggaatgt gttcgttacg
    14161 cagttgctgt ttggcttccg ccagtttttc tttcacccat aagatctctt ccattgagga
    14221 gatcatcggg atcatgattt tcaggctgcc gtgagcagag gcacggagga tcgaccgtag
    14281 ttgtgtggtg aacagcgagg cgtattcttc ataaatacgc accgcgcgat aaccgaggaa
    14341 cgggttagct tctgccggaa tgttcagata atcaacgggt ttgtcaccgc caatatccat
    14401 agtgcgcaca ataatgctgc gtccgttggc ggactccagc gcttgacaaa aaatgttgta
    14461 caactcgctt tcgcccggtg cgctggtgcg atccatatag agcatttcag tgcggaacaa
    14521 accgacgcct tccgcaccat tgccgaatgc ggcctgcgct tccacggagt gagcgatgtt
    14581 agcggcaatt tcaatgcgga taccgtcagc ggtacgggct tgttgggtca gccagacacg
    14641 ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc tgataataac gagctacggc
    14701 ttcccctggc tcaaccacaa tcgccccggc gttgccgtcg atataaatcg tttgatgctg
    14761 ccacggagta agggcatcaa tatctacacc aaccagtgtc ggaatgttga acgaacgggc
    14821 aagaattacg gtatgtgagg tggtgccgcc acttttcagt aacagacctt tgaggtgatt
    14881 tttatccagt tcgaggaact ggctgggggt tagttcatca gccatacaaa tggcgggctg
    14941 cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag atttgctgaa gcagctggaa
    15001 gcagacatcg cgtacgtcca gggcacgttc ttgcaggtag ctgctgctgg aacgggaaaa
    15061 ctcttcacaa aagtgattcg cactggcgac aatcgcttcg gcgcagctta atccggcgct
    15121 gacgcctgcc agtaaatgtt cgtgcaggga agtatcgcca gccagggatc ggtgcgcttc
    15181 cagaatcgcg ctggtggcac cgtcgctatc cagcagacga aactcaatgt ttttcagtac
    15241 cagcgtcagg ccgttttcca gtgcggattg ctcggcgtca acgtctttgg ctgcgggaag
    15301 attacccagc gcattgagat ctaaagaaga gatcggcgtc agaatgccgc ctgcgctacc
    15361 gctgcacacg gtgcgggcgc ggataatttg cggattcaga ttggtcagtg aaaccggcag
    15421 tggttccagt tcgtcagatt taacttccgc cagcggcgca tcgcagtggg ggaattcatc
    15481 gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg tcggccccgg aaatcagtaa
    15541 ctggcagtta tcgcccgcca gcgtgtcggt gccaatcagc gcaagggcgc ttttggcgtt
    15601 gcccttgcgg tcagtgcgca ggttatgcca ctcaatttgt gatgaaaaag tattacacag
    15661 cgtttcaacg tggcttgccg gacgtgcatg tacgccgtta ggtagctcac aaataaattc
    15721 cacaatcagg gccattgcct ctctcccggt acggttttct gtttacagga taagagcgca
    15781 cggcaacggc ctgccatgtg acaaatctgc caaaagctgg acaaatgtaa tgtaaccgtc
    15841 aatttgcgac gcgtctcaca agacgccgtt ttgcggcatg cttccggatt atcgcaagtt
    15901 atgaggcgga tcgcattttt gtactgatat tacaaaagtc cagtaaatgg cctttttatc
    15961 cactgtttgc tccgcctgcg attgcctatt gtttcctcca tcaaaaatat ggacatgggc
    16021 tttcgcaagc ccgggagcag cttatgaatg agttggtgca gatcctgaaa aatacccgtc
    16081 agcatttaat gacgggcgtt tcacacatga ttcccttcgt ggtatcgggc ggtattttgc
    16141 tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc ggatgccgta gccgatccga
    16201 atctgaaaaa actgtttgat atcggcgttg caggtttgac gctgatggtg cctttcctcg
    16261 ccgcttacat cggttactcc attgcagagc gttctgcgct ggctccgtgc gctatcggtg
    16321 cctgggttgg taacagcttt ggtgcgggct tctttggtgc actgatcgcc gggattatcg
    16381 gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca taaagttctg cgctcggtga
    16441 tgcccatctt catcattccg atcgtcggca cactgattac cgcaggcatc atgatgtggg
    16501 gtctgggcga gcctgtaggg gcgttgacca acagcctgac tcagtggctt caggggatgc
    16561 agcagggcag cattgttatg ctggcggtga tcatgggtct gatgctggcg ttcgatatgg
    16621 gcggtccggt taacaaagtg gcctatgcct tcatgctgat ttgcgttgct cagggtgttt
    16681 ataccgtggt ggccattgct gccgtgggta tttgtatccc gccgctgggg atggggctgg
    16741 cgacgctgat tggtcgtaaa aatttctccg cagaagagcg cgaaaccggt aaagcggcgc
    16801 tggtgatggg gtgcgttggg gttactgaag gggcgattcc tttcgccgca gccgatccgt
    16861 tacgtgtaat tccttccatc atggttggtt cagtttgtgg tgcagtaact gcggcgctgg
    16921 tcggtgcgca gtgctatgca ggctggggtg gtctgattgt gctgccggtg gttgaaggta
    16981 agttgggtta tatcgcagca gtggctgtcg gggcggttgt gacggcagtc tgcgtgaacg
    17041 tgctgaaaag tctggcgcgt aaaaatggat cttctactga tgaaaaagaa gacgacctgg
    17101 atttggattt tgaaattaat taattgagga acgagccatg acgaaaatta ttgcagtaac
    17161 cgcgtgtcca tctggtgttg cccatactta tatggctgca gaggcgctgg aaagtgccgc
    17221 gaaagcgaaa ggctgggaag tgaaagtaga aacccagggc tcgattggtc tggaaaacga
    17281 actgactgcg gaagacgtgg cgagcgccga tatggtgatt ctgactaaag atatcggcat
    17341 caagtttgaa gagcgttttg ctggcaagac catcgtgcgc gtcaacatca gcgatgcggt
    17401 aaaacgtgct gatgccatta tgagcaaaat tgaagctcac ctggcgcaaa ccgcttaagt
    17461 tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg agttcctcat gacgaatcgt
    17521 atctctcgcc tcaaaactgc actgtttgcc aatacccgtg aaatctcgct ggagcgtgcg
    17581 ctgctttata ccgccagcca tcggcaaacc gaaggcgaac cggtgatatt gcgccgggcg
    17641 aaagcaacag cgtatatcct tgaacatgtt gaaatttcga ttcgtgatga agaactgatt
    17701 gccggtaacc gcaccgtaaa accgcgcgcc gggattatgt cgccggaaat ggacccttac
    17761 tggctgctga aagagctggg tcaattcccg acgcgtccgc aggaccgttt tgctatcagc
    17821 gaagaagata aacgtatcta ccgcgaagag ttgttcccgt actgggaaaa acgttcgatg
    17881 aaagatttca tcaacgggca gatgacggat gaagtaaaag ccgcgactag cacgcagatt
    17941 ttcagcatca accagacgga taaaggccag gggcacatta ttattgatta cccgcgcctg
    18001 ctgaatcacg ggctggggga gctggtggca cagatgcagc aacattgtca gcaacagccg
    18061 gagaatcact tttatcaggc agcgctgtta ctgctcgaag cctcgcagaa acacattttg
    18121 cgttacgccg aactggcgga aacgatggcg gcaagctgca ctgatgccca gcgtcgcgaa
    18181 gaactgttga ctattgcgga gatctcccgc cataacgccg aacataaacc gcagacgttc
    18241 tggcaggcgt gccagttatt ctggtacatg aacatcattc tgcaatacga atccaacgcc
    18301 agttcgctgt ctttagggcg cttcgaccag tatatgttgc cgttctatca ggcatcttta
    18361 acccagggcg aagatccggc gttcctgaaa gaactgctcg aatctttatg ggtgaaatgc
    18421 aacgacattg tgctgttgcg ctccaccagc agcgcgcgtt atttcgcagg tttcccgact
    18481 ggctataccg cactgctcgg cgggttaacc gagaacggac gtagcgcggt gaacgtgctt
    18541 tcgttcctct gcctcgatgc ctatcaaagc gtacaattac cgcaaccgaa cctaggggtg
    18601 cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa ccgcagaaac cattcgcctc
    18661 ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg tgccagcgtt cctcaaccgt
    18721 ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg gctgtgtgga attatctatt
    18781 cccggcagaa cctacggctt gcatgacatc gcgatgttta atctgctgaa agtgatggaa
    18841 atctgcctgc atgaaaatga aggcaatgct gcgctgactt atgaaggttt actggagcag
    18901 atccgcgcca agatcagcca ctacatcacc ctgatggttg agggcagcaa tatttgtgat
    18961 atcggccatc gcgactgggc acctgtaccg ctgctctcgt cttttatcag cgattgtctg
    19021 gaaaaaggtc gcgatattac cgatggcggc gcgcgttata acttctccgg cgtacaggga
    19081 attggtatcg ccaacctgag cgattctctc catgcgttga aagggatggt ttttgatcaa
    19141 cagcgtttaa gttttgacga attgctgtcg gtattaaaag ccaactttgc aacgccagaa
    19201 ggcgaaaaag tccgcgctcg cttaattaac cgctttgaga aatacggtaa cgatatcgac
    19261 gaggtgggta acattagcgc cgaactgttg cgccactact gcaaagaagt ggaaaaatac
    19321 cagaacccgc gcggcggtta cttcacgccg ggatcgtata ccgtttctgc tcacgtcccg
    19381 ttgggatcgg tggttggcgc gacgccagac ggtcgttttg ccggagaaca gttggcagac
    19441 ggcggcttgt cacccatgct gggccaggac gcacaagggc caacagcggt actgaagtca
    19501 gtcagtaagc tcgataacac gctgctgtct aacggtacgt tgctgaacgt gaaattcact
    19561 ccggcgaccc tggaaggtga agcgggatta cgcaaactgg ccgacttctt acgggcgttt
    19621 acccagctta agttgcagca tattcagttt aacgtggtga acgccgacac gttgcgggaa
    19681 gcgcaacagc gcccacaaga ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc
    19741 ttctttgtcg aactgtcgaa ggagatccag gatgacatca tccgccggac agcgcatcag
    19801 ctgtaacgtt gtggaaacgc gccgcaatga tgtggcgcgc attttcaaca ttcagcgtta
    19861 ttcactgaat gacggcgagg gcattcgtac ggtggtcttt tttaaaggct gtccgcatct
    19921 ttgcccgtgg tgtgctaatc cggagtcgat ctccggcaaa atccagacgg tacgcagaga
    19981 ggcgaaatgt ctgcactgtg cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc
    20041 gtttgaacgg attggtcgcg atatcagcct tgacgcgctg gaacgggaag tgatgaaaga
    20101 tgacattttc tttcgcacgt ccggcggcgg cgtcacgctt tctggcggcg aagtgttaat
    20161 gcaggcggag tttgctaccc gttttttaca gcggctgcgg ttgtggggcg tgtcatgcgc
    20221 cattgaaact gccggagacg cgccagccag caagctgtta ccgctggcga aattgtgcga
    20281 tgaagtgttg ttcgatttaa aaattatgga cgcgactcag gcgcgggatg tggtgaagat
    20341 gaacctgcca cgcgtgctgg agaatctgcg tttgctggtg agtgagggcg tcaacgtgat
    20401 cccgcgttta ccgctgatcc ctggtttcac gctcagccgg gagaatatgc agcaggcgct
    20461 ggatgtactg atcccgctga aaatcaggca gatccatctg ttaccgtttc atcagtacgg
    20521 cgaaccgaaa taccgcctgc tggggaaaac atggtcgatg aaagaggtgc ctgcgccgtc
    20581 gtcagccgat gtgacaacga tgcgcgaaat ggcagaacgg gccggatttc aggttaccgt
    20641 gggaggttaa aatggcatac ctggtggcag taaccgcctg cgtaagcggc gtggcgcata
    20701 catatatggc ggcggaacgg ctggaaaagt tgtgcctgtt agagaagtgg ggagtcagca
    20761 ttgaaactca gggcgcgctg ggaacggaga atcgtttagc ggacgaggat atccgtcggg
    20821 cggatgttgc tctgttgatt acggatatcg agcttgccgg tgccgagcga tttgaacatt
    20881 gccgctatgt gcaatgcagc atctacgcat tcctgcgtga gccgcagcag gtaatgagcg
    20941 cggtgcgcaa agtgctttct gcgccgcagc aaacccatct tattctggag tagtcggttt
    21001 ttctgtcagt tggctgtggt actgccggcg atattccgac ggcgagcgtt cggtgttttt
    21061 acgaaacaga cggcagaagt agttgctgtc gacaaagccg caggcgtgcg ccacttcttt
    21121 taccttcagg tcatagcctt tcaataacgt cttagcgtgc tccagtcgcg tgtgattcag
    21181 gtattcgtta aaaccaatgg cccccgtttt ttgaaacagg tgcgagagat agtttggtga
    21241 aatatagaat gcctgtgcga cagattcgcg ggtaagcgcg gaggcatagc gttcgtcgat
    21301 ataatcgcga atagcttcga atagtgcctg gctgcgtgag gcggtctgga tttggctgcc
    21361 aagcaaatcg cggcagtggc taagcaggct ggcgacgata agccttgccg tttgctgctc
    21421 ctgcggctgc atttgcattt cattgagcgt ttgtagcaga aaagaaccta tgcgtgggcc
    21481 gcgtcgggcg acgtgttgct tcgccagatt ttgatattgt ttgccatccc attgcacgac
    21541 gctgaagccg agttgctgtt tgccaaacag cacgctaaag gtagtagcag gggcttgcca
    21601 ttgtggaaaa ttccagccac cagcggcaac gtacagcaca tcgccgggaa ctaacgttgc
    21661 tccagcgccg gtatcaacaa actcaccttc cagcacaatt tccagccgtg gaaaatcgac
    21721 ctgatacgca agatcgggca ccgggccgtt actgctggca aagtaaatct ggcgcaggga
    21781 cagcgggccg ttgatcaggc gggagagcag atagctgacg tcgtgataca tgttattccc
    21841 ttaggtatgg agacgggaaa acagtaactg gcattattaa aaaaggccac ctgaaaagtg
    21901 tggcctgagg ggagttcgat gtaaacattg cggcagttgt attgccggat gtggcgtaaa
    21961 cgccttatcc agtctaccgc cgcgatctgt aggccggata agacgcgtca agcgtcgcat
    22021 ccggcaaata gcgcctggct gataattact gattacctac ctggtcgcca tacggcagcg
    22081 tgtcgtaatc gatcagcgcg tttttcttgt acgggttacc aatccagcgg gtattttctt
    22141 tgaactgcgg attcaccact gaacgggttg gatcgtaacc gtcgtaagat aagcccgcta
    22201 aatctgacca ggtgtggatc agttccgcaa ggctgtattt acgatcaaca tcctgcgaga
    22261 aatcacgcgg atgagtcgct tgccattttt ccgacgtcca cagcaggaac ggaatggtgt
    22321 acatatgacg cgtcgggttg tcctcattac gcccctgggt tttatgaggc ggcgtgtcgt
    22381 aaacctcttc accgtggtca gagaagtaaa ccaggaaacc gttcgggtct gctgctttaa
    22441 agtctttaat caggctggca accacatgat cgttatacag gttagcgttg tcataatcgt
    22501 tatatgactc cagctcttcc gcgtttaatc ccggcggaac atgatcggta ttgccatcaa
    22561 acttgccctg attttccggg tagcggtatt tgtatttgat atgcgtaccc agcagatgaa
    22621 cgatgatcag tttcttcggc gcagggtcct tcagcacttc ctggaacggc ttcagcacgt
    22681 tagtgtcgta ttcacgcgca ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg
    22741 tctggcgcga aaataccgtc agcatggtat tgcgggcggt catcgtctgc tggttggtga
    22801 tccagaaggt tttataaccc gcctgtttca tcatgttcat cagcgacggc tgcgtcagat
    22861 acagatccgg gttcttttca ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt
    22921 acggacgaga cgtaactacg ttattaaaca cggtcagatt cggatcggtt ttatgcagcg
    22981 cattcagctc cggcgtggtt tcacgcggat aaccgtacag actcatgcgt ccacgctggg
    23041 ttgactcgcc aatcaccagc actaaggtgc gcggttcgtt acccgattca tctttgaaat
    23101 tagccagcgg cggcaaggca ttattttcat tcagtagctt tgttagcgag tttagttgct
    23161 gacgatactg ataatagccg gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg
    23221 ccaggttatc caacgttttc tcaaacggct tgtttttgat aaacatattc atggcgatcg
    23281 gatgcagaat caagccataa agcagggcaa aagagacaac ataacgccac ggctttggaa
    23341 tatagaccgg gcgcaggcgt gtccacagca gaactgccac cgccgtatag gccagcgcga
    23401 taagcacaat tttcaggctg aaatactggc ttaaatactc gctggcttcg ttggtgttgg
    23461 tttcgaacat cacaaacaga acgctctgcg agaactcctg accgtagatg acgtagtagc
    23521 acagcgccgc cagagaggcc gcccatagca ccacgccgat cactgcggca ataattttga
    23581 tccgcttcgg aaagaggaat accggaatta accacagcga gctgaataac agcgagtcgc
    23641 gaatgccgtt ggtgccacta taaccactga tgtaaataat ggcctgtagc agagtagaga
    23701 aaaaccaaaa gtagagcaat gcccaaccca gggctttcca gctaaaaaga ggtttagcct
    23761 ggacttctgt ggaatgcata gtaagaacct gtcttgaaaa aaatatcgcc gaatttaccg
    23821 acaattcctt aaggatatct gaagggatat ccagaatttg aataaaatgc agacagaaat
    23881 atattgaaaa cgagggtgtt agaacagaag tatttcagaa aaccctcgcg caaaagcacg
    23941 agggtttgca ggagaggaag attagccggt attacgcata cctgccgcaa tcccggcaat
    24001 agtgaccatt aacgcctgct cgacgcgagg atccggttcc tggccttctt tttctgcctg
    24061 gcgggagcgg tgcagcaact cggcttgcaa tacgttcagc gggtcggtgt aaatattccg
    24121 tagctgaata gactctgcaa tccacggcag atcggccatc agatgggaat cgttggcaat
    24181 cgccagcacc actttgatgt cttcttcttg cagattgcgt aactctttac ctaacggcca
    24241 cagtgttttg tctaccaggc gttggtcata gtattccgcc agccacaggt ctgctttggc
    24301 gaagaccatc tccagcatgc cgagacgcgt cgagaagaat ggccaatcgc ggcacatagc
    24361 ctccagctcg ctctgtttgc catcttcgac cactttttgc agcgccgtac ctgcacccag
    24421 ccaggcgggg agcatcagac ggttttgcgt ccaggcgaag atccacggaa tggcgcgtag
    24481 tgactcgacg ccgccggttg ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc
    24541 cagttcctgt tccggcgtag cggagcggaa gtaaggcaca aaatctttgt tttcacgtac
    24601 gtagccacgg tagagatcgc aggagatgac tgatagttca tccataatgc gacgccagct
    24661 ctctttcggc tcaggtggtg gcagcaggtt ggcttccagt atcgccccgg tgtaaagcga
    24721 caggctgctg acggtgattt ctggcagacc atatttaaag cggatcatct cgccctgttc
    24781 agtcacgcgc aggccacctt tcaggcttcc tggcggttgt gacagcagcg ccgcatgagc
    24841 aggtgcgccg ccgcgaccaa tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc
    24901 cgctttttcg caggttttga ttaatgcatc ctgtgcctga tattgcgccc aggacgctgc
    24961 catcacgccc gcatcttttg ctgagtcgga atagccaatc atcaccatct gtttgccctg
    25021 aatcaggccg cgataccagt cgatattcag caactgagtc atgacatcgt tggcgttgtt
    25081 caggtcatcg agagtttcaa acagcggagc aaccggcatc gcaaacccga tacccgcttc
    25141 tttcagcagc aggtggacag ccagcacgtc ggacggcgtt ttcgccatcg agatcacgta
    25201 ggcggcaatg gagccttgcg gtgcttcggc aatcacctgg caggtatcga gcacttcgcg
    25261 cgtttcggcg cttggttgcc agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg
    25321 gatcaggaac gcctgtttgt cggcctctga ccagctttcg tagtcgccga taccgaggta
    25381 gcgggtcagc tcgcccagcg cttcggtatg acgcgtgctc tcctgacgga tatcaatacg
    25441 gaccagcggt acgccgaaac atttcacgcg gcgcagggtg tcgagcagat cgccgttggc
    25501 gataataccc atgccacacg cctgaagtga ctggtagcaa gcgtagagcg gttcccacag
    25561 ttcttcgttt tgtgtcagca ggccttctgg ttttggcagt tcttcgcctt tcaggcgcgc
    25621 ttccagccat gcctgtgtcg ccatcaggcg agaacgcagg tttttcatca gatagcgata
    25681 cggttctgcg gcaccttctt cgccaaccag cgccagcagt tcaggggtcg cttcaaccat
    25741 cgacagttca gaaaccagaa cctgaatatc tttcaggaac agatcggtgg ctttccagcg
    25801 gctgagcagc aggacgtggc gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc
    25861 accgcccatc cacgaggtaa aacgtaccgg aacaaattcg acgggcagtt tgtagccgag
    25921 gttctcttcc agttgttcgt tcagttcgcg caggtaattc ggtacgcctt gccacaggct
    25981 gttttctact acggcaaagc cccatttggc ttcatctacc gggcttggac gcagcttacg
    26041 gatttcatcg gtatgccatg actgggcgat caactggcgc aggcgacgca tcagctggtt
    26101 gtgttcgtag tcagcgatat ctttgttatc gagctgtttt aagcaggcgt tcacttccac
    26161 cattttgtgg atcagtgtac gacgggtaat ttcggttggg tgagccgtga ggaccagttc
    26221 cagcgacagc gattccactg cttttttgat ggtgtcttcg ctcagttccg gctggttttt
    26281 cagtttacgc agggtgcggg cgatcacttc cgggttgctg gcagcttcgc ctttcggcga
    26341 aatgctgtgg tattgctcgg cggtgttggc caggttcagg aactgactaa acgcacgcgc
    26401 aacgggcagc agctcgtcgt tcgacaaatt ttgtaaggtg gtgagtaact cctggcggtt
    26461 agcatcattg ccagcgcgtg aagatttgga caacttacgg atagtttcta cgcgttcaag
    26521 aatgtgttct cccaacgcat ccttgatggt ttctcccagc actttgccga gcatactgac
    26581 attactacgc aatgcggaat attgttcgtt catattaccc cagacacccc atcttatcgt
    26641 ttgatagccc tgtatccttc acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg
    26701 tcgtctttta taaagccacg taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca
    26761 aacgaataaa tagcaggaat ttacgtcatt aaattcacga cgctttaaat aagcgtaact
    26821 tatgggaatg ttaaaaaatc gcctcaagta acaccaaagg tgtaggtcgg ataagatgtg
    26881 caagcatcgc atccgacatt attgcggcac tggagtttgg caacagtgcc ggatgcggcg
    26941 caagcgcctt attcggccta cgttttaatg ccagcaaaaa tggtgaatga cctgggttat
    27001 cagttcgcgg gtgggcttga taaaccgtgt ttccagatat tcatcaggct gatgagcctg
    27061 attaattgag ccaggcccca gaaccagcgt cgggcataac gtttgaataa acggcgcttc
    27121 ggtacagtaa ttcaccactt cggtttttgc tccgagcaat ttctcaacca cttcaaccag
    27181 ttgatgattc ggtgggcatt catagccagg gatcggcgga tgcagctcgt cgaccgtcag
    27241 acgacccggc cagcgttcgc tcaccggagc caatgcatcg ttgagcaaac cattaagttc
    27301 attgagtgtc atgccaggca gcggacgaat atccatatgc aactcacacc aagcgcaaat
    27361 acggttagaa gcgtcgccac cgtgaatatg cccgaggttg agcgtagggt atggcacggt
    27421 aaacgcttcg tagtgataac gttctttcag gttatcgcgc aattgcaaaa tatgcccgat
    27481 ggcgtcgtgc attagttcta tagcgttaac tccgcgtgct ggatcgctgg agtgccccga
    27541 ctggccctga atacggatgg cgttagagat atgaccttta tgtgcgcgta ccggttgtag
    27601 tgacgtcggt tcgccaatga tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata
    27661 acgcgctccg gccatactgg tttcttcatc agcagtcgcc agaatgtaga gcggtttttt
    27721 cagtttcgtg acgtcgacat cgcgtagcgc atcaaggata aacgcaaaaa agcctttcat
    27781 gtcggcggtg cctaagccgt aaagcttgcc gtcatgctcc gtcagtgtaa acggatcgcg
    27841 cgtccagcga ccgtcatcaa atggcaccgt atcggtatgc cccgccagca acaagccgcc
    27901 agccccctgt ccggtacttg ccagcatatt aaatttgttg cgagttcctg gtacaggctg
    27961 cacttccaca ttgaagccta aatctttaaa ccagtccgcc agcagagtga ttaaatctgc
    28021 attgctttga tcgagtgcct cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg
    28081 gtaaatctcg ataaatggcg gtaatttgtt tttcattgtt gacacacctc tggtcatgat
    28141 agtatcaata ttcatgcagt atttatgaat aaaaatacac taacgttgag cgtaataaaa
    28201 cccaccagcc gtaaggtgaa tgttttacgt ttaacctggc aaccagacat aagaaggtga
    28261 atagccccga tgttgaatac gctgattgtg ggtgccagcg gctacgctgg cgcagagcta
    28321 gtgacctgtg taaatcgcca tccgcatatg aacataaccg ctttgactgt ttcagcgcaa
    28381 agcaatgatg cgggaaagtt aatctccgat ttgcatccgc agctaaaagg catcgttgat
    28441 ctgccgttgc agccgatgtc ggatatcagc gagtttagcc ctggggtgga cgtggtgttt
    28501 ctcgccaccg cccatgaagt tagccacgat ttagcgccgc agtttcttga cgcgggctgc
    28561 gtggtgttcg acctttccgg cgcgtttcgt gttaacgacg ccaccttcta tgaaaaatat
    28621 tacggcttta cccatcaata cccggaactg ctggaacagg cagcctacgg tctggcggag
    28681 tggtgcggta ataaattaaa agaagcgaat ttgattgcgg tgccaggctg ttatccgacg
    28741 gcagcacagt tggcgctgaa accgttgatt gatgccgatc ttcttgacct caatcagtgg
    28801 ccggtgatca acgccaccag cggcgtgagc ggtgcaggac gtaaggcagc gatttcaaat
    28861 agtttttgtg aagttagcct gcaaccgtat ggcgtcttta cccatcgcca tcaaccagag
    28921 atcgccacac acctcggtgc tgacgttatc ttcaccccgc atctgggcaa tttcccgcgc
    28981 ggcattctcg aaaccattac ctgccgcctg aaaccgggtg tgacccaggc gcaagtggcg
    29041 caagcgttac agcaggcgta tgcccataaa ccgctggtgc ggctgtatga caaaggcgtt
    29101 ccggcgctga aaaatgtcgt tgggctacca ttttgcgata tcgggtttgc cgttcagggc
    29161 gaacatctga ttattgtggc gaccgaagac aacttactga aaggcgcggc ggcacaagcg
    29221 gtacagtgcg ccaatattcg tttcggctat gcggaaacgc agtctcttat ttaagggtgc
    29281 aatgatgaat ccattaatta tcaaactggg tggcgtactg ctggatagtg aagaggcgct
    29341 ggaacgtctg tttagcgcac tggtgaatta tcgtgagtca catcagcgtc cgctggtgat
    29401 tgtgcacggc ggcggttgcg tggtggatga gctgatgaaa gggctgaatc tgccggtgaa
    29461 aaagaaaaat ggcctgcggg tgacgcctgc tgatcagatt gacattatca ccggagcact
    29521 ggcgggaacg gcaaataaaa ccctgttggc atgggcgaag aaacatcaga ttgcggccgt
    29581 aggtttgttt ctcggtgacg gcgacagcgt caaagtgacc cagcttgatg aagagttagg
    29641 tcatgttgga ctggcgcagc caggttcgcc taagcttatc aactccttgc tggagaacgg
    29701 ttatctgccg gtggtcagct ccattggcgt aacagacgaa gggcaactga tgaacgtcaa
    29761 tgccgaccag gcggcaacgg cgctggcggc aacgctgggc gcggatctga ttttgctctc
    29821 cgacgtcagt ggcattctcg acggcaaagg gcaacgcatt gccgaaatga ccgccgcgaa
    29881 agcagaacaa ctgattgagc agggcattat tactgacggc atgatagtga aagtgaacgc
    29941 ggcgctggat gctgcgcgca cgttgggccg tccggtagat atcgcctcct ggcgtcatgc
    30001 ggagcagctt ccggcactgt ttaacggtat gccgatgggt acgcggattt tagcttaagt
    30061 tttgttggcc ggaggcgcag ctttccggca ttgaatttca aaataaggaa acagagttat
    30121 ggcactttgg ggcgggcgtt ttacccaggc agcagatcaa cggttcaaac aattcaacga
    30181 ctcactgcgc tttgattacc gtctggcgga gcaggatatt gttggctctg tggcctggtc
    30241 caaagccctg gtcacggtcg gcgtgttaac cgcagaagag caggcgcaac tggaagaggc
    30301 gctgaacgtg ctgctggaag atgttcgcgc caggccacaa caaatccttg aaagcgacgc
    30361 cgaagatatc catagctggg tggaaggcaa actgatcgac aaagtgggcc agttaggcaa
    30421 aaagctgcat accgggcgta gtcgtaatga tcaggtagcg actgacctga aactgtggtg
    30481 caaagatacc gttagtgagt tactgacggc taaccggcag ctgcaatcgg cgctggtgga
    30541 aaccgcacaa aacaatcagg acgcggtcat gccaggttac actcacctgc aacgcgccca
    30601 gccggtgacg ttcgcgcact ggtgcctggc ctatgttgag atgctggcgc gtgatgaaag
    30661 ccgtttgcag gatgcgctta agcgtctgga tgtcagcccg ttaggttgtg gcgcgctggc
    30721 gggaacggct tatgaaatcg accgtgaaca gttagcgggc tggctgggat ttgcttcagc
    30781 aacccgtaac agtctcgaca gcgtttctga ccgtgaccac gtgctggaac tgctttctgc
    30841 tgccgctatc ggcatggtgc atctgtcgcg ttttgctgaa gatctgattt tctttaacac
    30901 cggcgaagcg gggtttgtgg agctttctga ccgcgtgact tccggttcat cattaatgcc
    30961 gcagaagaaa aacccggatg cgctggagct gattcgcggt aaatgcggtc gggtgcaggg
    31021 cgcgttaacc ggcatgatga tgacgctgaa aggtttgccg ctggcttaca acaaagatat
    31081 gcaggaagac aaagaaggtc tgttcgacgc gctcgatacc tggttggact gcctgcatat
    31141 ggcggcgctg gtgctggacg gcattcaggt gaaacgcccg cgttgccagg aagcggcgca
    31201 acagggttac gcgaacgcca ctgaactggc ggattatctg gtggcgaaag gtgtgccgtt
    31261 ccgcgaggca caccatattg tgggtgaagc tgtggtggaa gccattcgtc agggcaaacc
    31321 gctggaggag ctgccgctca ccgagttgca gaaatttagt ccggtgattg gtgaagatgt
    31381 ctatccgatt ctgtcgctgc aatcgtgcct cgacaagcgt gcggcaaaag gcggcgtctc
    31441 accacagcag gtggcgcagg cgattgcttt tgcaagggcg cggttggggt aagaacattt
    31501 atatgtacaa atttgagcct ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat
    31561 cctggagatc cgcaaaagtt cacgttggct ttagttattc gagttgagaa actctcgaaa
    31621 cgggcagtga cttcaagggt taaaagaggt gccgctccgt ttctgtgagc aattatcagt
    31681 cagaatgctt gatagggata atcgttcatt gctattctac ctatcgccat gaactatcgt
    31741 ggcgatggag gatggataat gaatattcgt gatcttgagt acctggtggc attggctgaa
    31801 caccgccatt ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg
    31861 caaattcgta agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa
    31921 gtgttgttca cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag
    31981 gtgaaagtcc ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcat
    32041 attggtttga ttcccacggt tggaccgtac ctgctaccgc atattatccc gatgctgcac
    32101 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
    32161 caactggaca gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca
    32221 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agaccacccg
    32281 tgggcgaacc gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg
    32341 gaagatggtc actgtttgcg cgatcaggca atgggttttt gctttgaagc cggggcggat
    32401 gaagatacac acttccgcgc gaccagcctg gagacgctgc gcaacatggt ggcggcaggt
    32461 agcgggatca ctttactgcc agcgctggct gtgccgccgg agcgcaaacg cgatggggtt
    32521 gtttatctgc cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct
    32581 ggctcaccgc tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc aagaatggat
    32641 ggccatttcg ataaagtgtt aaaacaggcg gtttaaaccg tttaacgcag ctacccgata
    32701 ggcttccgcc atcgtcgggt agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc
    32761 gccacctttc tgttccataa tcgcctgacc gatatgaata atttcggcag cgcgctcgcc
    32821 aaagcagtga atgcccagaa tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc
    32881 cacgttcatg ccgacgattt gtgcacgtgc cagatgtttg aactgggcgc ggcccacttc
    32941 atatggcact ttcatcgcgg tcagctgctg ttcggttttg cccacagagc tgatttccgg
    33001 gatggtgtaa atgccggtag ggatatcttc aatcagatgc gcggtggctt cgccttttac
    33061 cagcgcctgc gcggcaatgc gcccctggtc ataggccgct gatgccaggc tcggataacc
    33121 aatcacgtcg cccaccgcgt aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt
    33181 cagctgtccg cgactgtcag tttccagccc aatgttctgt aacgccagcg aatcggtatt
    33241 accggtacga ccgttggcat agagcaggca gtcagctttc agttttttac ccgacttcag
    33301 atgcatgatc acaccgtcgt cacagccttc gatcttctcg tactcttcgt tgtgacgaat
    33361 cactacgcca ctgttccaga agtgatagga gagagaatct gacatctctt gatcgagaaa
    33421 tgccagcagg cgatcgcggg tgttgatcag atccactttt acatccatac cgcggaagat
    33481 cgacgcatat tcacagccga tcactccagc accatagata agtacatggc gcggttcgtg
    33541 gtgcatgctg agaattgagt cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt
    33601 tggatgatat ggacgagagc cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac
    33661 gctgccgtcc gggcaatcca gcgccaacgt atgctcgtca acaaagcgag cgttcccctg
    33721 caatatttca cagtgattac gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat
    33781 cacgttatcg gcatggttaa ggatatcggc aaaagaagag cggagcagtc gggaatggtc
    33841 gctgtatagt gggttttgat tgaattctat aatgcggctg acggcgtgac ggagagcttt
    33901 cgacgggatg gtgccccagt gggtgcaacc gccgccaaca ttttgataac gctcgataac
    33961 tgcgacgcgc gcaccttgct taaccaggcc cattgcagcg ccttcgccgc cggggccgga
    34021 acctattact atggcatcgt aatcgtagga atgtggcatg gtagggctta cctgttctta
    34081 tacataaaag caacagaatg gtaacatttt atcgcgggta agccaattga tccccgtcat
    34141 taatctggct atagcgtgag cggcctttgc tttgtctgcc tccttcttaa cccatttatt
    34201 cactattaat cgctttttat tatggctgat agttttatct tgccgaagcg tgcttttttg
    34261 ttattgtctt tgtgaaatct tcttataaaa caaacatata aatattttat attgatgagt
    34321 tatttgtatt tacaccatca ataaaatccg ttaattagcc tgtttataag tgaataaagg
    34381 aatagccgat gatcgaacac tatattcgag ggtttgagga agaactgcgt gagatccgcc
    34441 atcagatcca cgaaaacccg gagctgggat tacaagaatt taaaaccagt gctttggttg
    34501 cggaaaagct gcgtcagtgg ggctatgaag tagagcaagg actggcgacg acggggatag
    34561 tggccacgtt aaaagtgggc gacggagaga aaagtattgg cctgcgggcg gatatggatg
    34621 cgctgccgat ttatgaaaat agtggcaagc cctgggccag taaacatcct ggtttaatgc
    34681 acgcctgcgg gcatgacggg cataccacca tactgctggg ggccgcgcgt tattttgccg
    34741 aaacccgccg ttttaacggc acgttacgtc ttatcttcca gcctgcggaa gagatgatta
    34801 atggcggtga gatcatggtt aaagaggggc tttttgatca tttcccctgc gatgtcattt
    34861 tcggcatgca taacatgccc ggtctgccgg tgggtaagtt ttatttccag ccaggggcac
    34921 tgatggcgtc aatggatcag ttccatatta cagttcgtgg ttgtggcgga cacggtgcga
    34981 tcccgcacaa agccatcgat ccggtgctag ttgccgcaca tatcaccacc gcattacaaa
    35041 gcattgtgtc acgcaatgtc gatccgctgg aagcggcggt aattaccgtc ggcagcattg
    35101 ttgcgggtga ggcggctaac gtcattcctg acagtgctga gatgaaaatc agcgtccgct
    35161 ctcttagccg cgatacccga caacttcttc tgacccgtat tcctgctctg gcacaagccc
    35221 aggctgccag ctttggcgct acggctgagg ttacgcatgt taacggtaca ccagtattag
    35281 taaatgacga ggagatggcg cgcttcgcct ggcaggtggc gtgtaaaacg tttggtgaag
    35341 atcgggccga gtttggcatc aagcccctga tgggcagtga agatttctct ttcatgctgg
    35401 aagcccaacc caaaggcggt ttcctgttat tcggtaatgg cgatgtcggg gaaggttcct
    35461 gcatggtgca taacccgggt tacgacttca acgatgcaag tctggtcccg gcaagcagct
    35521 actggggcgc actggtagaa gcctggctgc aataaataaa acaacacaca acaacacttt
    35581 ttggcaagga acatggaatg acggcgatcg taaatgaaac cgtgagtgtg aagaaaaagc
    35641 ccagcggcag gcgggtgatt tttgcctcgg cgtttggcaa tgcgctcgaa ttttttgatt
    35701 ttggcgttta caactttttt gtggtctata tcagcacgct attttttccg cccagtgcag
    35761 ataaaaacgt cgcgctactg ctggcgtttg ccactttcgg cgtgagcttt tttatgcgcc
    35821 cgctcggcgg cattattgtg ggggcctggg ccgatcgttt cggacgcaaa cccgcgatgg
    35881 tgttcaccat tgcgctgatg agtcttggta cactgatgat cggcatcgca ccgacgtatg
    35941 aaacggcggg ctattgggga accgcgacgc tggtgctagc gcgtctaatt cagggcgttg
    36001 cggcaggagg cgaagtcggt gcctcaatgt ctttgttggt tgaatcagct ccggcaaatc
    36061 gtcgcggttt ttacagcagt tggtcactgg cgactcaagg cctggcaaca acttttggag
    36121 gtgttgtcgc tttaggttta agtgcatggc tgccctttgc gaccggttca gaaaccgtca
    36181 tggctgaatg gggctggcgt gtgccgtttt ttattggcgt gttattagcg cctgtcggct
    36241 gttggttgcg tttgagtctg gaaaatgatg ttcctgagcc agcacataat aagaaggccg
    36301 cagccagcga aagcgccttc tccttactta tgcaacataa agcgactatc gctaacggta
    36361 tcttgctcgc tattggtagt acagtcgcaa cctatatctc cctcttttat tacggtacat
    36421 gggcggcgaa gtatttagga atgaaccaaa actattctca tgcagcgatg ttactggcgg
    36481 gcgttattac gtttgtgggt gcgctgctgg tggggatgtt gtgcgattcg gtcgggcgta
    36541 aaaagctgat tttaatctcc cgtgtgatgg tgctgatctg tagctggccg tcattctggc
    36601 tgttggtgaa ttacccaagc ccaggcatgt tgctgacggt ggttttcgtg atggtcagct
    36661 ttaccacgct tggcggtgtg ccagtgatgt tgttgatctc tgaactgttg ccgaaacgga
    36721 ttcgggcatt gggttttgcg ctggtttata gcattggtgt ggcaatcttt ggcggttttg
    36781 ctcaatattt cgctacgcaa tccatcgtgt tgctggatag tttgacggct ccggcgtggt
    36841 atctgggtgg aggcacgttg ctgtcgatgt tggcgttgtt gtacgtaaaa gaaccggcca
    36901 aagaattgca gtaaatatgt tttattgcgt taccgttcat tcacaatact ggagcaatcc
    36961 agtatgttca ttctctggta tagtgccagc agtacttttg gcaaggattc agacatcgtg
    37021 atgggcgtaa gagcgcaaca aaaagaaaaa acccgccgtt cgctggtgga agccgcattt
    37081 agccaattaa gtgctgaacg cagcttcgcc agcctgagtt tgcgtgaagt ggcgcgtgaa
    37141 gcgggcattg ctcccacctc tttttatcgg catttccgag acgtagacga actgggtctg
    37201 accatggttg atgagagcgg tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt
    37261 atcgccaaag gcgggagtgt gatccgcacc tcggtctcca catttatgga gttcatcggt
    37321 aataatccta acgccttccg gttattattg cgggaacgct ccggcacctc cgctgcgttt
    37381 cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg aacttgcgga ctatctggaa
    37441 ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt
    37501 gtcttcagtg cgggtgccga ggcgttggac gtcggcgttg aacaacgtcg gcaattagaa
    37561 gagcgactgg tactgcaact gcgaatgatt tcgaaagggg cttattactg gtatcgccgt
    37621 gaacaagaga aaaccgcaat tattccggga aatgtgaagg acgagtaatg aaacaagcaa
    37681 atcaagatag aggtacgctg ctgctggcgt tggttgctgg cttatcgatt aatggtactt
    37741 tcgcagcgct gtttagctcc attgtgccat tttctgtatt cccgattatt tccctggtgc
    37801 tgacggttta ctgcctgcat caacgttatc ttaatcgcac catgccggta ggcttgccgg
    37861 gtctggcagc tgcctgtttt attctcggcg tactgctgta cagcacggta gttcgtgcgg
    37921 aatatccgga tatcggctct aacttcttcc cggcagtact ctccgtcatt atggtgttct
    37981 ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc tgagtaatcg gtatcatgcc
    38041 ggatgctggc gcatccggca tgggttttac ttcgcggtca gtaatacgcc gcactccata
    38101 tggtgcgtgt aggggaactg atcaaacaga gccagacgtt cgaccttgtg cgtctggctt
    38161 aatgtttcca gatttttgca taacgtttcc ggattgcagg agatatacag aatacgtgga
    38221 tacgcctgca ccattttctc ggtttcactg tccagaccgc tgcgaggtgg gtcgacaaaa
    38281 atggtttcgc actgataact ctttaagtcg atcccttgca ggcggttaaa ctcgcgcacg
    38341 ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa taatttgtac gttatcaata
    38401 tggttagcgg caatgttgta ttgcgcggca gcgactgatg gcttggcgat ttcggtggct
    38461 aatacccgat caaaattgcg cgccagcgct aatgagaagt taccgttgcc gcagtacagc
    38521 tccagtaaat cgccttttga gcctttggtt acgtccagcg cccattccag catctgaata
    38581 ttcatcgccg cgttcggctg ggtaaagctg ttttctacct gacgatagat catctctttc
    38641 cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca gcgcgatttt ggttttcgtt
    38701 gcccgaccaa tcagatgcac attcagattc tgcgcgcgca gtgcatcgcg cagggcttcc
    38761 gcttcctgac gccactcatc atccagcttt ttatggtaca gcagggaaac caccgcctga
    38821 ttactcagcg tggtgaggta atcaatctgg aacaacttgt ggcgcagaac gggattatta
    38881 cgcacacccg caatcatcgc cgtcatcaac tggttgatga gttcactggc ggcggggaag
    38941 ctatccaccc ggatacggct tttggtttgt tgatcgaaaa tgatgtggta caggtcatcg
    39001 ccatcgtgcc agatgcggaa ctccgcacgc atccggtaat gactgaccgg cgagcgaaac
    39061 acttccggaa ccaggtcaga aaacggtgcc atcatacttt gcaaacgtac cactttttcg
    39121 gctaactgcg cttcatactg ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa
    39181 aaattaaagt acgcggcgat tgtagggatt gctcatcaga tgtccagatc ttgatgaatt
    39241 cctatttgtg agctacgtct ggacagtaac ttgttacaac ctgtagcatc cacttgccgg
    39301 tcctgtgagt taatagggaa tccagtgcga atctggagct gacgcgcagc ggtaaggaaa
    39361 ggtgcgatga ttgcgttatg cggacactgc cattcggtgg gaagtcatca tctcttagta
    39421 tcttagatac ccctccaagc ccgaagacct gccggccaac gtcgcatctg gttctcatca
    39481 tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat tgtggatgct ttacaatgat
    39541 taaaaaagct tcgctgctga cggcgtgttc tgtcacagcc ttttccgctt gggcacagga
    39601 taccagcccg gatactctcg tcgttactgc taaccgtttt gaacagccgc gcagcactgt
    39661 gcttgcacca accaccgttg tgacgcgtca ggatatcgac cgctggcagt cgacctcggt
    39721 taatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc caaaacggcg gttcaggtca
    39781 gctctcatct atttttattc gcggtacaaa tgccagtcat gtgttggtgt taattgatgg
    39841 cgtacgcctg aatctggcgg gggtgagtgg ttctgccgac cttagccagt tccctattgc
    39901 gcttgtccag cgtgttgaat atatccgtgg gccacgctcc gccgtttatg gttccgatgc
    39961 aataggtggg gtggtgaata tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc
    40021 agggtgggga agcaatagtt atcaaaacta tgatgtctct acacagcaac aactggggga
    40081 taagacacga gtaacgttgt tgggcgatta tgcccatact catggttatg atgttgttgc
    40141 ctatggtaat accggaacgc aagcgcagcc agataacgat ggttttttaa gtaaaacgct
    40201 ttatggcgcg ctggagcata actttactga tgcctggagc ggctttgtgc gcggctatgg
    40261 ctatgataac cgtaccaatt atgacgcgta ttattctccg ggttcaccat tggtcgatac
    40321 ccgtaaactc tatagtcaaa gttgggacgc cgggctgcga tataacggcg aactgattaa
    40381 atcacaactc attaccagct atagccatag caaagattac aactacgatc cccattatgg
    40441 tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa
    40501 caacatcatc attggccacg gtaatgttgg tgcgggtgtt gactggcaga agcagagcac
    40561 ggcaccgggc acagcttatg ttaaggatgg atatgatcaa cgtaataccg gcatctatct
    40621 gaccgggctg caacaagtcg gcgattttac ctttgaaggc gcagcacgca gcgacgataa
    40681 ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc ggttgggaat tcatcgaagg
    40741 ttatcgcttc attgcttcct acgggacatc ttataaggca ccaaatctgg ggcaactgta
    40801 tggcttctac ggaaatccga atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt
    40861 tgaaggctta accgctgggg tgaactggcg tatttccgga tatcgtaacg atgtcagtga
    40921 cttgatcgat tatgatgatc acaccctgaa atattacaac gaagggaaag cgcggattaa
    40981 gggcgtcgag gcgaccgcca attttgatac cggaccactg acgcatactg tgagttatga
    41041 ttatgtcgat gcgcgcaatg caattaccga cacgccgttg ttacgccgtg ctaaacagca
    41101 ggtgaaatac cagctcgact ggcagttgta tgacttcgac tggggtatta cttatcagta
    41161 tttaggcact cgctatgata aggattactc atcttatcct tatcaaaccg ttaaaatggg
    41221 cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc acctctcacc tgacagttcg
    41281 tggtaaaata gccaacctgt tcgacaaaga ttatgagaca gtctatggct accaaactgc
    41341 aggacgggaa tacaccttgt ctggcagcta caccttctga accacgtccc accgtgctgg
    41401 tgtttgactc cggcgtcggt gggttgtcgg tctatgacga gatccggcat ctcttaccgg
    41461 atctccatta catttatgct ttcgataacg tcgctttccc gtatggcgaa aaaagcgaag
    41521 cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt gcaagaacgt tatccccttg
    41581 cgctggctgt ggtcgcttgc aacactgcca gtaccgtttc acttcctgca ttacgcgaaa
    41641 agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg
    41701 caaatggcat tgtcggatta ctggcaaccc gcggaacagt taaacgttct tatactcatg
    41761 agctgatcgc gcgtttcgct aatgaatgcc agatagaaat gctgggctcg gcagagatgg
    41821 ttgagttggc tgaagcgaag ctgcatggcg aagatgtttc tctggatgca ctaaaacgta
    41881 tcctacgccc gtggttaaga atgaaagagc cgccagatac cgttgtattg ggttgcaccc
    41941 atttccctct actacaagaa gaactgttac aagtgctgcc agagggaacc cggctggtgg
    42001 attctggcgc agcgattgct cgccgaacgg cctggttgtt agaacatgaa gccccggatg
    42061 caaaatctgc cgatgcgaat attgcctttt gtatggcaat gacgccagaa gctgaacaat
    42121 tattgcccgt tttacagcgt tacggcttcg aaacgctcga aaaactggca gttttaggct
    42181 gatttggttg aatgttgcgc ggtcggaaaa ttattttaaa tttcctcttg tcaggccgga
    42241 ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca
    42301 gcggggttct cctgagaatt ccgacagaga aagcaaaaat aaatgcttga ctctgtagc
//
LOCUS       DAZGXS010000024        41273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGXS010000024 DAZGXS010000000
VERSION     DAZGXS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            483..932
                     /gene="alaE"
                     /locus_tag="ACHKG5_001443"
     CDS             483..932
                     /gene="alaE"
                     /locus_tag="ACHKG5_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0867574.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(969..1313)
                     /locus_tag="ACHKG5_001444"
     CDS             complement(969..1313)
                     /locus_tag="ACHKG5_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0867575.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1465..1794
                     /locus_tag="ACHKG5_001445"
     CDS             1465..1794
                     /locus_tag="ACHKG5_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0867576.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARNAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1944..3278)
                     /locus_tag="ACHKG5_001446"
     CDS             complement(1944..3278)
                     /locus_tag="ACHKG5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0867577.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3366..3797
                     /locus_tag="ACHKG5_001447"
     CDS             3366..3797
                     /locus_tag="ACHKG5_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0867578.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            4006..4251
                     /gene="nrdH"
                     /locus_tag="ACHKG5_001448"
     CDS             4006..4251
                     /gene="nrdH"
                     /locus_tag="ACHKG5_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0867579.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4248..4658
                     /gene="nrdI"
                     /locus_tag="ACHKG5_001449"
     CDS             4248..4658
                     /gene="nrdI"
                     /locus_tag="ACHKG5_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0867580.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4631..6817
                     /gene="nrdE"
                     /locus_tag="ACHKG5_001450"
     CDS             4631..6817
                     /gene="nrdE"
                     /locus_tag="ACHKG5_001450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0867581.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLAQGDETLAQGDETLAL
                     QLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRG
                     GGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAH
                     HPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
                     PFADVAISQHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPV
                     AGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVE
                     TAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFT
                     NLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGEL
                     FARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVE
                     IRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFP
                     DTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6827..7786
                     /gene="nrdF"
                     /locus_tag="ACHKG5_001451"
     CDS             6827..7786
                     /gene="nrdF"
                     /locus_tag="ACHKG5_001451"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0867582.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            8142..9344
                     /gene="proV"
                     /locus_tag="ACHKG5_001452"
     CDS             8142..9344
                     /gene="proV"
                     /locus_tag="ACHKG5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0867583.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            9337..10401
                     /gene="proW"
                     /locus_tag="ACHKG5_001453"
     CDS             9337..10401
                     /gene="proW"
                     /locus_tag="ACHKG5_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0867584.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10458..11450
                     /gene="proX"
                     /locus_tag="ACHKG5_001454"
     CDS             10458..11450
                     /gene="proX"
                     /locus_tag="ACHKG5_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0867585.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11642..12826
                     /locus_tag="ACHKG5_001455"
     CDS             11642..12826
                     /locus_tag="ACHKG5_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867586.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12950..13687
                     /gene="ygaZ"
                     /locus_tag="ACHKG5_001456"
     CDS             12950..13687
                     /gene="ygaZ"
                     /locus_tag="ACHKG5_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0867587.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13677..14012
                     /gene="ygaH"
                     /locus_tag="ACHKG5_001457"
     CDS             13677..14012
                     /gene="ygaH"
                     /locus_tag="ACHKG5_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0867588.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            14103..14633
                     /gene="emrR"
                     /locus_tag="ACHKG5_001458"
     CDS             14103..14633
                     /gene="emrR"
                     /locus_tag="ACHKG5_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0867589.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14760..15932
                     /gene="emrA"
                     /locus_tag="ACHKG5_001459"
     CDS             14760..15932
                     /gene="emrA"
                     /locus_tag="ACHKG5_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0867590.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15949..17487
                     /gene="emrB"
                     /locus_tag="ACHKG5_001460"
     CDS             15949..17487
                     /gene="emrB"
                     /locus_tag="ACHKG5_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0867591.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17551..18066)
                     /gene="luxS"
                     /locus_tag="ACHKG5_001461"
     CDS             complement(17551..18066)
                     /gene="luxS"
                     /locus_tag="ACHKG5_001461"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0867592.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(18216..19772)
                     /gene="gshA"
                     /locus_tag="ACHKG5_001462"
     CDS             complement(18216..19772)
                     /gene="gshA"
                     /locus_tag="ACHKG5_001462"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0867593.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19845..20273)
                     /locus_tag="ACHKG5_001463"
     CDS             complement(19845..20273)
                     /locus_tag="ACHKG5_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP0867594.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(20270..20836)
                     /gene="yqaB"
                     /locus_tag="ACHKG5_001464"
     CDS             complement(20270..20836)
                     /gene="yqaB"
                     /locus_tag="ACHKG5_001464"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0867595.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(21117..21193)
                     /locus_tag="ACHKG5_001465"
     tRNA            complement(21117..21193)
                     /locus_tag="ACHKG5_001465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21157..21159),aa:Arg,seq:acg)
     gene            complement(21296..21372)
                     /locus_tag="ACHKG5_001466"
     tRNA            complement(21296..21372)
                     /locus_tag="ACHKG5_001466"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21336..21338),aa:Arg,seq:acg)
     gene            complement(21435..21511)
                     /locus_tag="ACHKG5_001467"
     tRNA            complement(21435..21511)
                     /locus_tag="ACHKG5_001467"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21475..21477),aa:Arg,seq:acg)
     gene            complement(21576..21652)
                     /locus_tag="ACHKG5_001468"
     tRNA            complement(21576..21652)
                     /locus_tag="ACHKG5_001468"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21616..21618),aa:Arg,seq:acg)
     gene            complement(21656..21748)
                     /locus_tag="ACHKG5_001469"
     tRNA            complement(21656..21748)
                     /locus_tag="ACHKG5_001469"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21712..21714),aa:Ser,seq:gct)
     gene            complement(22064..22249)
                     /gene="csrA"
                     /locus_tag="ACHKG5_001470"
     CDS             complement(22064..22249)
                     /gene="csrA"
                     /locus_tag="ACHKG5_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0867596.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22484..25114)
                     /gene="alaS"
                     /locus_tag="ACHKG5_001471"
     CDS             complement(22484..25114)
                     /gene="alaS"
                     /locus_tag="ACHKG5_001471"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0867597.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(25242..25742)
                     /gene="recX"
                     /locus_tag="ACHKG5_001472"
     CDS             complement(25242..25742)
                     /gene="recX"
                     /locus_tag="ACHKG5_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0867598.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25810..26871)
                     /gene="recA"
                     /locus_tag="ACHKG5_001473"
     CDS             complement(25810..26871)
                     /gene="recA"
                     /locus_tag="ACHKG5_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0867599.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26951..27448)
                     /gene="pncC"
                     /locus_tag="ACHKG5_001474"
     CDS             complement(26951..27448)
                     /gene="pncC"
                     /locus_tag="ACHKG5_001474"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0867600.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27593..28678)
                     /gene="mltB"
                     /locus_tag="ACHKG5_001475"
     CDS             complement(27593..28678)
                     /gene="mltB"
                     /locus_tag="ACHKG5_001475"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0867601.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28933..29496
                     /gene="srlA"
                     /locus_tag="ACHKG5_001476"
                     /gene_synonym="gutA"
     CDS             28933..29496
                     /gene="srlA"
                     /locus_tag="ACHKG5_001476"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0867602.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            29493..30454
                     /gene="srlE"
                     /locus_tag="ACHKG5_001477"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             29493..30454
                     /gene="srlE"
                     /locus_tag="ACHKG5_001477"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            30465..30836
                     /gene="srlB"
                     /locus_tag="ACHKG5_001478"
                     /gene_synonym="gutB"
     CDS             30465..30836
                     /gene="srlB"
                     /locus_tag="ACHKG5_001478"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0867603.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30840..31619
                     /gene="srlD"
                     /locus_tag="ACHKG5_001479"
     CDS             30840..31619
                     /gene="srlD"
                     /locus_tag="ACHKG5_001479"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0867604.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31725..32084
                     /gene="gutM"
                     /locus_tag="ACHKG5_001480"
     CDS             31725..32084
                     /gene="gutM"
                     /locus_tag="ACHKG5_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0867605.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            32151..32924
                     /gene="srlR"
                     /locus_tag="ACHKG5_001481"
                     /gene_synonym="gutR"
     CDS             32151..32924
                     /gene="srlR"
                     /locus_tag="ACHKG5_001481"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0867606.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            32917..33882
                     /gene="gutQ"
                     /locus_tag="ACHKG5_001482"
     CDS             32917..33882
                     /gene="gutQ"
                     /locus_tag="ACHKG5_001482"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP0867607.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33879..35393)
                     /gene="norR"
                     /locus_tag="ACHKG5_001483"
     CDS             complement(33879..35393)
                     /gene="norR"
                     /locus_tag="ACHKG5_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0867608.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35580..37019
                     /gene="norV"
                     /locus_tag="ACHKG5_001484"
     CDS             35580..37019
                     /gene="norV"
                     /locus_tag="ACHKG5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0867609.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            37016..38149
                     /gene="norW"
                     /locus_tag="ACHKG5_001485"
     CDS             37016..38149
                     /gene="norW"
                     /locus_tag="ACHKG5_001485"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0867610.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(38277..40529)
                     /gene="hypF"
                     /locus_tag="ACHKG5_001486"
     CDS             complement(38277..40529)
                     /gene="hypF"
                     /locus_tag="ACHKG5_001486"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0867611.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40682..41209)
                     /gene="hydN"
                     /locus_tag="ACHKG5_001487"
     CDS             complement(40682..41209)
                     /gene="hydN"
                     /locus_tag="ACHKG5_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0867612.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         9818 a        11090 c        10459 g         9906 t
ORIGIN      
        1 agattattgg tgaacagaac ggcgctctct gctcgctaat tccgcaagcc tgacaaaacc
       61 aaagtgaata aaaatgcaat ataacagaat aaaataaaac acccctcact ggtgctttct
      121 gctgttaatg tacattttct ggcataaaaa atgctgtaaa tgacccggtc acgacataca
      181 ccgaattctc tacgcttata gcgttacctc acccccaaac tttcattagt gaaattaaag
      241 gaaaggtcat atccaattga tttaattgaa aataaatcat atcggcatgt aaaaatccac
      301 aatgcacaaa aaacgagccg ttacggaata ttttatctac aaaaactgac taaataaaaa
      361 tttttcacta attgattagt catagccagc gatatacgct atgcgaaaat gcagatggca
      421 atgagatcca ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta
      481 gcatgttctc accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg
      541 tttactgttc tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac
      601 agtcttttta ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg
      661 gtatgtaccg cgatctgttt atgcgcgcgg cacgcaaagt tagcccgtcg ggctggataa
      721 aaaatctggc ggatatcctg gcttatgtga cgttccagtc accggtgtat gtggcgatct
      781 tgttagtggt gggcgcagac tggcatcaga ttatggcggc ggtcagttca aacatcgttg
      841 tttcgatgtt gatgggggcg gtttatggct acttcctcga ttattgccgc cgactgttta
      901 aagtcagccg ttaccagcag gtaaaagcct gactcttctt ttcgcgactg gcatcgccag
      961 tcgcgtcttt aactggcttc gccaaataaa ccattcaaat aacgttcaag cgcaacacgc
     1021 gaactaaagc catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa atagagcgga
     1081 aattgctggt aatgatcgca agttttaata tcggaagcgg gttcagccag tgtcgaactg
     1141 cgttctttta atgttggatg aattaccaga gcggtacgtc ccattcgcgc ttcgcgatta
     1201 acatagacat aattttcccc acggcggtaa ccatacgctt tctgggttac cacatcgaca
     1261 gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata catatttgat
     1321 cctcgttatt atcaaccgcg ggcctacctt acctgattgc gcattcacaa cgctttcaga
     1381 aaagtccata aagccgcgat gaacctcagt taagaaaatt atggtctaca ctgaaaatta
     1441 catcgaattc taatggggga tcatatgttt aaccgaccga accgcaacga cgttgatgat
     1501 ggcgtgcagg atatccagaa tgatgtcaat caattagctg acagcctcga atctgtattg
     1561 aagtcctggg gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa agctcaggca
     1621 ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca agccgcgcgc
     1681 aatgccgtag gctgcgcgga ttcttttgtt cgtgaaagac cctggtgtag tgtgggaact
     1741 gctgctgcgg taggtatttt tatcggtgcg ttgttgagca tgcgtaaatc gtagtgagac
     1801 gatgacaagc agtagtctga cttctgaaaa gtactcagtg cgtcaacgcc ttgtccgcct
     1861 acaggcctga aaaacatagc gcatcagggg attttgccct ggtcaccagt cttataggac
     1921 cccgaagcct ggcttcgggg ttattaattt aaagcactgc gcaattgctg tgctacctgt
     1981 cgcgccatac tttcgctaac gatattcgta aacgagagca ataatcctcc gtcctcgctt
     2041 gagcggatac gccagtcgct aagtgcctgt acggcaagac cagcagcatt ggctttatgc
     2101 acgaatgtca cgtcattacc tgctagtttg atcaccatct ggataccgcc tttttgcggc
     2161 acaacctgaa atccctgctg cgtaagtgcc tgctcaatcc actgccgtcg ctgggcataa
     2221 tgctggcgca ttttcttcag atgccgccag aaatgcccct cacggatgaa atctgccagt
     2281 gtgttctgcc acagtacggg tacagcacat ggttccagtg acgcctgctg ccggaattgc
     2341 gaaatctgct ccaccggcac caccagccac gcacagcgca atgcaggaaa tagcgctttg
     2401 ctgaatgttc cggcataaat cacccgctgc ggcgcgtcga gactttttag tgccggtaac
     2461 ggcttaccgt gatagcgaaa ctcactgtcg taatcatctt caataatcca tgcctgacta
     2521 cgctctgccc attccagtat ctgataccta cgcgctaaag agagtgccac acccagcggg
     2581 ctttggtgtg ccggagtaat cagggcaaaa cgcgcatcag gataattttg tattccgctt
     2641 gtgacatcca gtccgttgtc atcaaccggc acaggtaaaa tttccacacc gtggcgagtg
     2701 acaatcgggc gaatcagcgg aaagccggga tcttccatcc acatcccgtc tcccggttgc
     2761 gccagagcgt gcagaattaa agccattgag gccgcataac cgtgagtgat aaagacctgc
     2821 tctggctgac aatcaatccc acgtgaaacg cgtaaataat caacaatagc ctcgcgcaag
     2881 gccgcctcgc cgcagacatc gcctaatgcc aaatcaaagc gcgtctgggt acgcagacgg
     2941 cggcccatca cccgcgccca caattcacgc ggaaaaagat caagtgcagg tagccccatc
     3001 tgaaaaggca gcggctgctg actttcacca gcaaaagcca cgacatccac agcagactgt
     3061 tgcggatgta tctgctcact gacaaaggta cccgcctgtc cccgcctgac cagccatccc
     3121 tgcgccacca gctcagcata agcattttcg acagtggatc gagagacacc cagctcctgc
     3181 gaccaggtac ggctcgaagg tagccttgcg ccaggtttca gttttccttg ctcgataacc
     3241 gtttttaact gacgggcgat atcctgataa cgtggcatat ggtctgcttt tttaggtaat
     3301 ttatggctct ctttagcagt ccattatagt gctacattgc gggcgttaat aagaggagaa
     3361 actgtatgac tacattacgt cagccttact acgaacttag cccggcagtg tataacgcac
     3421 tggtgcaggc caaaacggca ctggaaaata gcacgctgga taccacgttg atggagttaa
     3481 tttatttgcg cgtctcgcaa atcaacggct gcgcattttg tctggagatg cacagcaaag
     3541 cattgcgcaa atccggtgtg ccacaacaca aactggacgc cctggcaggc tggcgcgtaa
     3601 gccatcattt tgatgaacgc gagcgagcgg cgctggcatg ggcggaatct gtgaccgata
     3661 ttgccagaac ccatgcggaa gacgaggttt atcagccttt gcttgagcat ttcagcgcag
     3721 cagaaatcag cgacttaacg tttgccatcg ggctgatgaa ttgttttaac cgtctggccg
     3781 tttccatgcg gatgtaattt gctgttgccg ggtatgcgcc cggcaaaaaa acacaaaatc
     3841 ccctaccccg ttgagccatt caccggtggc gtaatcaatc taaaattacc atatcttgtg
     3901 tggttgaatc ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac
     3961 gcgtatcatc gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt
     4021 tacactcgta acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc
     4081 tttgattttg aaatgattaa tgtcgatcgc gttcctgaag cggcagaagc gttgcgtgct
     4141 cagggctttc gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc tggtttccgt
     4201 ccggacatga ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg
     4261 tctacttctc cagcagctcc gaaaacacgc agcgttttat cgaacgttta ggtctgcccg
     4321 cggtgcgcat cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga
     4381 tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct
     4441 ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt tattgcttcg ggtaatcgca
     4501 actttggtga ggcgtatggc cgcgccggag atgtgattgc ccggaaatgc ggcgtgccgt
     4561 ggctgtaccg ttttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag
     4621 taaccgaatt ttggcaacga caaccgcaga atgcctgacg cagaaaacga tggattacca
     4681 cgcgctgaat gcgatgctta acctctacga tagcgcaggt cgcattcagt tcgataaaga
     4741 ccgccaggcc gttgacgcct ttatggcgac gcatgtgcgt ccgaacagtg tgaccttcag
     4801 tagccagcag cagcgcctga actggctggt caacgaaggt tactatgatg aaagcgttct
     4861 taatcgctac tctcgcgact ttgtcattac gctgtttgcc cacgcacaca ccagcggttt
     4921 tcgtttccag acattcctcg gggcatggaa gttttacacc agctatacgt tgaagacatt
     4981 cgacggtaaa cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct
     5041 ggcacaaggc gatgagacgc tggcacaagg cgatgagacg ctggcacaag gcgatgagac
     5101 gctggcgttg caactgacag atgaaatgct gtcaggacgc tttcagccag ccacgccaac
     5161 attcctcaac tgcggtaagc agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat
     5221 tgaagacaat atggagtcga ttggtcgggc ggtaaattcc gcactgcaac tgtcgaaacg
     5281 cggcggcggc gtagcatttt tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg
     5341 tattgaaaat caatcttctg gcgtaattcc ggtgatgaaa atgctggaag acgcattttc
     5401 ctatgccaac caactcggcg ctcgtcaggg ggctggtgca gtctatttac atgctcatca
     5461 tcccgatatt ctgcgttttc tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat
     5521 taaaacactg tcgcttggcg tggtgatccc ggatatcact ttccatctgg caaaagagaa
     5581 tgcgcagatg gcgctgtttt cgccttatga cgtagagcga gtttatggca agccgtttgc
     5641 cgatgtggcc atcagccaac actatgacga actggttgcc gatgaacgca ttcgcaaaaa
     5701 atacctcaac gcccgtgatt tcttccagcg actggcagaa atccagtttg agtccggcta
     5761 tccctacatc atgtatgaag acacggtaaa ccgtgctaac cctgtcgccg ggcgcataaa
     5821 tatgagtaat ctctgctcag aaattttgca ggttaacagc gcctcagagt atgacgagaa
     5881 tctcgactat gcccgcacag gccatgatat ctcctgcaat ttaggttcgt tgaatattgc
     5941 gcacaccatg gattcccccg attttgcccg cacggtagag actgccgtgc gcggtttaac
     6001 ggcagtatca gatatgagtc atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc
     6061 ctcgcacgcc atcggactgg ggcagatgaa tttacacggc tatctggcgc gagaaggcat
     6121 cgcttatggt tcgccggaag cactggattt caccaatctc tatttctatg ccatcacctg
     6181 gcacgcactg cgtacctcga tgttgctggc acgcgaacgc ggtgaaacct tcgccgggtt
     6241 caaacagtca cgctatgcca gtggtgaata ttttagccaa tatctgcaag ggaactggca
     6301 gccgaaaacg gggaaagttg gcgaactgtt tgcccgtagc ggtattacgt tacctacccg
     6361 tgagatgtgg gcgcaactgc gcgacgacgt gatgcgctac ggcatttaca accagaatct
     6421 tcaggcggtg ccgccaacag gttctatctc ttatatcaac catgctacgt cgagtattca
     6481 tccgattgtg gcgaaagtag agatacgcaa agagggcaaa acaggacgcg tttactaccc
     6541 tgccccgttt atgactaacg agaatctggc gctgtatcag gacgcttacg aaattggcgc
     6601 agaaaagatc atcgacacct acgcggaagc gactcgccat gtcgatcagg ggctgtcgct
     6661 gacgctgttt ttccccgata ccgccaccac tcgcgatatc aacaaagcgc agatttacgc
     6721 ctggcgcaaa ggtatcaaaa cgctctatta catccggctg cgtcagatgg cgctggaagg
     6781 cactgaaatt gaaggctgcg tctcctgtgc actttaagga atatctatga aactctcacg
     6841 tatcagcgcc atcaactgga acaagatatc tgacgataaa gatctggagg tgtggaatcg
     6901 cctgaccagc aatttctggc taccagaaaa ggtgccgctg tcgaacgata ttcctgcctg
     6961 gcagacatta actgtcgtag aacaacaact gacgatgcgc gtttttactg gcctgacgct
     7021 gctcgacacg ctgcaaaatg ttatcggcgc gccttcgctg atgcccgatg cactcacgcc
     7081 tcatgaagaa gcggtattat cgaatatcag ctttatggaa gcggttcatg cccgctctta
     7141 cagttcgatt ttctcgacgc tgtgccagac caaagatgtc gatgccgcct acgcctggag
     7201 tgaagaaaac gcgccgttgc agcgaaaagc acagattatt cagcaacatt accgcggtga
     7261 tgatccgctg aaaaagaaaa tcgccagtgt gtttcttgaa tcttttttgt tctattccgg
     7321 tttctggctg ccgatgtatt tttccagccg cggaaagctg accaataccg cggacctgat
     7381 ccgtctgatt atccgcgatg aagcagtcca cggttactac ataggctata aatatcagaa
     7441 aaacatggaa aagatatctc tgggacaacg tgaagagttg aagagtttcg ccttcgattt
     7501 gttgctggaa ctctacgaca acgagttgca atacaccgat gagctgtacg ccgaaacccc
     7561 gtgggctgac gatgtgaaag cgtttctctg ttacaacgcc aataaggctt tgatgaatct
     7621 gggctacgaa ccgttatttc ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc
     7681 gctttcgccg aatgccgatg aaaatcacga tttcttttcc ggttcaggct cctcttatgt
     7741 gatggggaaa gcggttgaaa cagaatatga agactggaat ttctgagggt gttattttca
     7801 aaaatatcac tacccgcgac agggaaataa ttcccgccaa atagcttttt atcacgcaaa
     7861 tattttgtgg tgatctacac tgatactctg ttgcattatt cgcctgaaac cacaatattc
     7921 aggcgttttt tcgctatctt tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc
     7981 ttatatcacg ggaaattccg gcgatttgct cgcatcaata ttcatgccac atttgccatc
     8041 aggggttgcc tcagattctc agtatgttag ggtagaaaaa agtgactatt tccattgggt
     8101 aatatatcga cataaacaaa taacaggaat ctttctattg catggcaatt aaattagaaa
     8161 ttaaaaatct ttataaaata tttggtgagc atccacagcg agcgttcaaa tatatcgaac
     8221 aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc gtaaaagacg
     8281 ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc ggctcgggta
     8341 aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg caagtactga
     8401 ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa
     8461 agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg ctggacaata
     8521 ctgcgtttgg tatggaattg gccggaatta atgccgaaga acgccgggaa aaagcccttg
     8581 atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccgaat gaactctctg
     8641 gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg gatattttat
     8701 taatggatga agccttctcg gcgctcgatc cattaattcg caccgagatg caggatgagc
     8761 tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac gatctcgatg
     8821 aagccatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg
     8881 gcacaccgga tgaaattatc aataatccgg cgaatgatta tgtccgtacc ttcttccgtg
     8941 gcgttgatat tagtcaggta ttcagtgcga aagatattgc ccgccggaca ccgaatggct
     9001 taattcgtaa aacccctggc ttcggcccac gttcggcact gaaattattg caggatgaag
     9061 atcgtgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg
     9121 attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg attgatgcgc
     9181 cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc ggacaggctc
     9241 cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa
     9301 tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg
     9361 gataccacgc cagcggcgga cagtgccgcg caatccgcag acgcctgggg tacaccggcg
     9421 actgcaccga ctgacggcgg tggtgctgac tggctgacca gtacgcctgc gccaaacgtc
     9481 gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga cagttgggtc
     9541 actgaaggga tcgactgggt cgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt
     9601 ccggttgatt atatcctcaa cggtttccag caattgctgc tgggtatgcc cgcaccggtg
     9661 gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat gggcgtggcg
     9721 acgctggttt cgctgattgc catcggcgca atcggtgcct ggtcgcaggc aatggttact
     9781 ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca tcggtttgcc gttggggatc
     9841 tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga tgccatgcag
     9901 accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat cggtaacgtg
     9961 ccgggcgtgg tggtgacgat catctttgct ctgccgccga ttatccgtct gaccattctg
    10021 gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg tgccagcccg
    10081 cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt
    10141 aaccagacgc tgatgctggc cctttctatg gtggtcatcg cctcgatgat tgccgtcggc
    10201 gggttgggtc agatggtact tcgtggtatc ggtcgtctgg atatggggct tgccaccgtt
    10261 ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc gtctgacgca ggccgttggg
    10321 cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt cggtctgctg
    10381 acccgcccat tcattaagta actctgcact tgcccggtga cgccgggcat atcaccctgc
    10441 caaaaaaagg aataacaatg cgacatagcg tactttttgc gacagcgttt gccacgctta
    10501 tctctacaca aacttttgct gccgacctgc cgggcaaagg cattactgtt aatccggttc
    10561 agagcaccat caccgaagaa actttccaga cgctgctggt cagccgcgcg ctggagaaat
    10621 taggttatac cgtcaataaa cccagcgaag tggattacaa cgttggctac acctcgcttg
    10681 cttccggcga tgcaaccttc accgccgtga actggacgcc actgcatgac aacatgtacg
    10741 aagctgccgg tggcgataag aaattttatc gtgaaggggt atttgttaac ggcgcggcac
    10801 agggttacct gatcgataag aaaaccgccg accagtacaa aatcaccaac atcgcacaac
    10861 tgaaagatcc gaagatcgcc aaactgttcg ataccaacgg cgacggaaaa gcggatttaa
    10921 ccggttgtaa ccctggctgg ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg
    10981 aactgaccaa taccgtgacg cataatcagg ggaactacgc ggcgatgatg gccgacacca
    11041 tcagtcgtta caaagagggc aaaccggtgt tttactacac ctggacgccg tactgggtga
    11101 gtaatgagct gaagccaggc aaagatgtgg tctggttgca ggtgccgttc tctgcactgc
    11161 cgggcgataa aaatgccgat accaaactgc cgaatggcgc gaattacggc ttcccggtca
    11221 gcaccatgca tatcgttgcc aataaagcct gggccgagaa aaacccggca gcagcgaaac
    11281 tgtttgccat tatgcagttg ccagtggcag atattaacgc ccagaacgcc attatgcatg
    11341 acggcaaagc ctcagaaggc gatattcagg gccacgttga tggctggatc aaagcccacc
    11401 agcaacagtt cgatggctgg gtgaatgagg cgttggcagc gcagaagtaa tttttattcg
    11461 ggcggataag gcgtttacgc cgcatccgcc gttctgtaca caatgcctga tgcgacgctg
    11521 gcgcgtctta tcatgcctac aaaccacacc gcacatttca acaatctatc tttcatccca
    11581 tattcatcaa catccgctat tattgatttc cagcttaatc atcacctgat gaacaaaaat
    11641 aatgaccaaa cctaatcatg agcttagccc ggcgctaatc gtgctgatgt ctatcgccac
    11701 cggtctggcg gtcgccagca actattacgc ccagccattg ctcgacacca tcgcgcgtaa
    11761 cttttccctt tccgccagtt cggcaggctt tattgttacc gccgcgcagt tgggctatgc
    11821 cgccggtctg ctgtttcttg ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt
    11881 ctcgatgacc ttactggcag cgggcggtat gttaatcact gccagcagtc agtcactggc
    11941 gatgatgatc ctcggtacgg cattaaccgg tttattctca gtcgtggcac aaattctggt
    12001 tccgctggca gcgacgctgg cttcaccgga taaacgcggc aaagtggttg gcaccattat
    12061 gagcggtctg ctgttgggga tattgctggc acgaaccgtt gccgggttac tggcaaacct
    12121 cggcggctgg cgcaccgtct tttgggttgc ctcggtgtta atggctctga tggcgctggc
    12181 attatggcgt ggtctgccac aaatgaaatc agaaacccac ctcaactacc cacagttgtt
    12241 gggttccgtt ttcagcatgt ttatcagcaa taagatcctg cgcacccgcg cgttgctggg
    12301 ctgcctgacc tttgccaact tcagcattct ctggacctca atggcctttt tgcttgccgc
    12361 tccacctttt aactacagcg atggtgtaat tggtctgttt gggcttgcgg gagctgccgg
    12421 agcgttgggc gctcgtccgg cgggcggttt tgccgacaag ggcaaatcgc accacaccac
    12481 aactttcggt ctgcttctgc tgttactttc atggctggca atctggtttg gtcacacttc
    12541 cgtactggcg ttgattatcg gcattctggt gctggacctc accgtacagg gcgtgcatat
    12601 cactaaccag acggtaattt atcgaataca tcctgatgcg cgtaatcgcc tgaccgcagg
    12661 ttacatgacc agctacttta ttggcggtgc cgccggttcg ctaatttcag cctcagcctg
    12721 gcaacatggc ggttgggctg gcgtttgtct ggctggcgcg acgattgccc tggttaactt
    12781 actggtctgg tggcgaggtt ttcatcgtca ggaagccgca aattaagcaa tcagcaaccg
    12841 ttttcgtggc tttacactta taagggtgtt aagaagccca tcagtctgat aaggttaaga
    12901 tattcattca gtctatttat aatattaaca atcgttaagc gtacactcta tggaaagccc
    12961 tactccacag cctgctcctg gttcggcgac cttcatggaa ggatgcaaag acagtttacc
    13021 gattgttatt agttatatcc cggtggcctt tgcgttcggt ctgaatgcga cccgtctggg
    13081 attctctcct ctcgaaagcg tttttttctc ctgcatcatt tatgcaggcg cgagccagtt
    13141 cgtcattacc gcgatgctgg cagcagggag tagtttgtgg gttgctgcac tgaccgtcat
    13201 ggcaatggat gttcgccatg ttttgtatgg cccgtcactg cgtagccgta ttattcagcg
    13261 tctgcaaaaa tcgaaaaccg ccctatgggc gtttggcctg acggatgagg tttttgccgc
    13321 cgccaccgca aaactggtac gcaataatcg ccgctggagc gagaactgga tgatcggcat
    13381 tgccttcagt tcatggtcat cgtgggtatt tggtacggta ataggggcat tctccggcag
    13441 cggcttgctg caaggttatc ccgccgttga agctgcatta ggttttatgc ttccggcact
    13501 ctttatgagt ttcctgctcg cctcttttca gcgcaaacaa tctctttgcg ttaccgcagc
    13561 gttagttggt gcccttgcag gcgtaacgct attttctatt cccgtcgcca ttctggcagg
    13621 cattgtctgt ggctgcctca ctgcgttaat ccaggcattc tggcaaggag cgcccgatga
    13681 gctatgaggt tctgctgctt gggttactgg ttggcgcggc gaattattgc ttccgctatt
    13741 tgccgctgcg cctgcgtgtg ggtaatgccc gcccaaccaa acgtggcgcg gtaggtattt
    13801 tgctcgacac cattggcatc gcctcgatat gcgctctgct ggttgtctct accgcaccag
    13861 aagtgatgca cgatacacgc cgtttcgtgc ccacgctggt cggcttcgcg gtactgggtg
    13921 ccagtttcta taaaacacgc agcattatca tcccaacact gcttagtgcg ctggcctatg
    13981 ggctcgcctg gaaagtgatg gcgattatat aacccacaag aatcattttt ctaaaacaat
    14041 acatttactt tatttgtcac tgtcgttact atatcggctg aaattaatga ggtcataccc
    14101 aaatggatag ttcgtttacg cccattgaac aaatgctaaa atttcgcgcc agccgccacg
    14161 aagattttcc ttatcaggag atccttctga ctcgtctttg catgcatatg caaagcaagc
    14221 tgctggagaa ccgcaataaa atgctgaagg ctcaggggat taacgagacg ttgtttatgg
    14281 cgttgattac gctggagtct caggaaaacc acagtattca gccttctgaa ttaagttgtg
    14341 ctcttggatc atcccgtacc aacgcgacgc gtattgccga tgaactggaa aaacgcggtt
    14401 ggatcgaacg tcgtgaaagc gataacgatc gccgctgcct gcatctgcaa ttaacggaaa
    14461 aaggtcacga gtttttgcgc gaggttttac caccgcagca taactgcctg catcaactct
    14521 ggtccgcgct cagcacaaca gaaaaagacc agctcgagca aatcacccgc aaattgctct
    14581 cccgtctcga ccagatggaa caagacggtg tggttctcga agcgatgagc taacgcgtca
    14641 tctcgctcaa aaatccagat ttataaaaga aaaaatgacc ggccagcatc gcaacatgct
    14701 ggcctttttg gcaagcaggt cggctcagcc gatgagttaa gaagatcgtg gagaacaata
    14761 tgagcgcaaa tgcggagact caaaccccgc agcaaccggt aaagaagagc ggcaaacgta
    14821 agcgtctgct cctccttctc accttgctct ttataattat tgccgtagcg atagggattt
    14881 attggttttt ggtactgcgt cacttcgaag aaaccgatga cgcatacgtg gcagggaatc
    14941 aaattcaaat tatgtctcag gtgtctggca gcgtgacgaa agtctgggcc gataacaccg
    15001 attttgtaaa agaaggcgac gtgctggtca ctctcgaccc gacagatgct cgccaggcgt
    15061 ttgaaaaagc caaaactgca ctggcttcca gcgttcgcca aacccaccag cagatgatta
    15121 acagcaagca gttgcaggcg aatattgagg tgcaaaaaat cgccctcgcg aaagcacaaa
    15181 gcgactacaa ccgccgtgtg ccgctgggca atgccaacct gattggtcgc gaagagctgc
    15241 aacacgcccg cgacgccgtt accagtgccc aggcgcaact ggacgtcgcg attcaacaat
    15301 acaatgccaa tcaggcgatg attctgggga ctaaactgga agatcagcct gccgtgcaac
    15361 aggctgccac cgaagtacgt aacgcctggc tggcgctgga gcgtactcgt attgtcagtc
    15421 cgatgaccgg ttatgtctcc cgccgcgcgg tacagcctgg ggcgcaaatt agcccaacga
    15481 cgccgctgat ggcggtcgtt ccagccacca atatgtgggt ggatgccaac tttaaagaga
    15541 cgcagattgc caatatgcgt atcggtcagc cggtcactat caccaccgat atttacggcg
    15601 atgatgtgaa atacaccggt aaagtggttg gtctggatat gggcacaggt agcgcgttct
    15661 cactgcttcc ggcgcaaaat gcgaccggta actggatcaa agtcgttcag cgtctgcctg
    15721 tgcgtatcga actggaccag aaacagctgg agcaataccc gctgcgtatc ggtttatcca
    15781 cgctggtgag cgtcaatacc actaaccgtg acggtcaggt actggcaaat aaagtacgtt
    15841 ccactccggt agcggtaagc accgcgcgtg aaatcagcct ggcacctgtc aataaactga
    15901 tcgacgatat cgtaaaagct aacgctggct aatccagagg tgcgtgtgat gcaacagcaa
    15961 aaaccgctgg aaggcgcgca actggtcatt atgacgattg cgctgtcact agcgacattc
    16021 atgcaggtgc tggactccac cattgctaac gtggcgatcc ccactatcgc cgggaatctt
    16081 ggctcatcgc tcagccaggg aacgtgggta atcacttctt tcggggtggc gaatgccatc
    16141 tcgatcccgc ttaccggctg gctggcaaag cgcgtcgggg aagtgaaact gttcctctgg
    16201 tcaaccatcg cctttgctat tgcgtcgtgg gcgtgtggtg tctccagcag cctgaatatg
    16261 ctgatcttct tccgcgtgat tcaggggatt gtcgccgggc cgttgatccc gctttcgcaa
    16321 agtctattgc tgaataatta cccgccagcc aaacgctcaa tcgcgctggc gttgtggtcg
    16381 atgacggtga ttgtcgcgcc aatttgcggc ccaatcctcg gcggatatat cagcgataat
    16441 taccactggg gctggatatt cttcatcaac gtgccgattg gcgtggcggt ggtgttgatg
    16501 acactgcaaa ctctgcgcgg acgtgaaacc cgcaccgaac ggcggcggat tgatgccgtg
    16561 gggctggcac tgctggttat tggtatcggc agcctgcaga ttatgctcga ccgcggtaaa
    16621 gagctggact ggttttcatc acaggaaatt atcatcctta ccgtggtggc ggtggtggct
    16681 atctgcttcc tgatcgtctg ggagctgacc gacgataacc cgatagtcga tctgtcgttg
    16741 tttaagtcgc gcaacttcac catcggctgt ttgtgtatca gcctcgcgta tatgctctac
    16801 ttcggcgcga ttgttctgct gccgcagttg ttgcaggagg tctacggtta cacggcgacc
    16861 tgggcaggtt tggcctctgc gccggtaggg attattccag tgatcctgtc gccgattatc
    16921 ggccgcttcg cgcataaact ggatatgcgc cgactggtaa ccttcagctt tattatgtat
    16981 gccgtctgct tctactggcg tgcctatacc tttgaaccag gtatggattt tggcgcgtcg
    17041 gcctggccgc agtttatcca ggggtttgcg gtggcctgct tctttatgcc gctgaccacc
    17101 attacgctgt ctggtttgcc accggaacga ctggcggcgg catcgagcct ctccaacttt
    17161 acgcgtacgc tggcggggtc tatcggcacg tcgataacca caaccatgtg gaccaaccgc
    17221 gagtcgatgc accatgcgca gttgactgag tcggtaaacc cgttcaaccc gaatgcccag
    17281 gcgatgtaca gtcaactgga agggcttggg atgacacaac agcaggcatc aggctggatt
    17341 gcccagcaga tcaccaatca ggggctgatt atttccgcca atgagatctt ctggatgtcg
    17401 gccgggatat tcctcgtctt gctggggctg gtgtggtttg ctaaaccgcc ctttggcgca
    17461 ggtggcggcg gaggcggtgc gcactaagta caactaagcc agttcatttg aactggcttt
    17521 tttcaattaa ttgtgaagat agtttactga ctaaatgtgc agttcctgca acttctcttt
    17581 cggcagcgcc agttcttcgt tgctgttgat gcgcacgtca cgttccagaa tgctacgcgc
    17641 aatatcctgc gcttcctgca acgagtgcat ctggtaagtg ccacactggt agacgttcag
    17701 ctccggaatc tgattctgat cctgcacttt cagcacgtct tccattgccg ctttccagac
    17761 atcagcaaca cgctgctcat ctggcgtacc aatcagactc atataaaaac cggtgcggca
    17821 gcccattggc gagatatcga taatctctac accattaccg ttaagatggt tacgcataaa
    17881 accagcaaac aggtgctcca gggtatggat ccctctttct ggcatcactt ctttgttcgg
    17941 cacgcagaag cgcagatcga acacggtgat tgcgtcgcca tgcggggtgt tcattgtttt
    18001 cgccacccga actgcaggcg cttccatccg ggtatgatcg actgtgaagc tatctaacaa
    18061 cggcatttag ccacctccgg taattttttt aaaaattttc tgaactcttt cttcccaggc
    18121 gagtctgagt atatgaaaga cgcgcatttg ttatcatcat ccctgaattc agagatgaaa
    18181 ttttggccac tcatgagtgg cctttttctt ttctgtcagg cgtgtttttc cagccacacc
    18241 gcaaacggtt cggtatctgc ggcttccatt tcctgctgac ggcgttcaga tgcctcgcgc
    18301 tcggctacaa aatcctcttc gcgcagaatt tccagcggct cttcacgcag cagattacgg
    18361 tacgcttcag caaacgcttt gcctgttccg ccaataccag tatcaatcat agaccttaag
    18421 atacgggcag agaaagtcag atcgggatta tcgaagcagg caaccagttc atcacacact
    18481 ttctgatacg cttcgccgcc gttaatgcta tccagcgttt gcgcgacgcg tttcagatcg
    18541 cggaacagat ctttacccac ctgcggtaac gggaactgtg cggtttcgca gccgataccc
    18601 agcgtcagac ccggtttgcg accttcgagg atcacccggt tccagttaac gcgtgtacag
    18661 gcaagttcgc tactgctcat ttccggtgca tcagccagcg cacaccagac cataaacagg
    18721 tcgaggaatc gcacctgctg ttcatctaca ccaatcggcg agaacgggtt gatgtccagc
    18781 gaacgcactt caatatattc aatgccgcca cgtaacagcg catcagaagg cgactcgccg
    18841 ctgcgggtaa cgcgttttgg acgaatcggc gcgtacagtt cgttttcaat ctgcagcacg
    18901 ttgctgttga tttgcagcct cttaccgtct ttatcaatac caatcttcgc gtactcttcc
    18961 gatggcgttt tgattgcctg tttcaggccc gctacgtact cgtaaagatc gttgaaggta
    19021 ataccaagat tgctttgcga tttattggta tagccgagat cgctcaaacg aagagaggtc
    19081 gcatacggca ggtaatacat accgcactcg gttttctcaa acggcagcga cgttggttta
    19141 ccttgcagga atgaagagca tattgccgga gacgcgccaa acagataagg aatgacccaa
    19201 ccgaagcgat agtagttgcg aataacgcgg aaatagcccg cagagatttt ctctttagca
    19261 tcagcgcccg aaatatcgcc acacttcgct tgccagaatg ccataggcaa agagaagttg
    19321 taatgcacgc cggagatggt ttgcatcagc gcgccgtagc gatttttcag cccttcacgg
    19381 tacagcgttt taaagcgtcc ggtgttagaa gtgccgtact gcgccagttc gatgtcctga
    19441 ccttctgcga tgtagcatgg catacttaac ggccacatcc gctcatcgcc catattgcgc
    19501 gccgtataac gatgcagatc gcgcataaag gtcagcatat gttcaatatc accatccact
    19561 ggtgtaatga attccagcaa tgcttccgca aaatcggtag taatccattt atgcgtcagt
    19621 gcggaaccta atgcttcagg atgacctgtt gttgccagtg tgccatcagc attaacacgc
    19681 aaagtttcgc gctccagccc acgctgtatc ccctttaacg cctgaggatg tttttccagc
    19741 caggccagcg cctgtgatac gtccgggatc aaattgacct cccgcctgtc aaaatcgttt
    19801 taattagcat aactgtaatg gtgaccatat gtgcaggcct acaattagtg ccaccacatc
    19861 atgccctgaa cggtcgctgc tgcaactgca acatagcgta acgctttacc aaggcacaaa
    19921 aaaaagatta ccggccccca ggagatgcgc atccatcccg ctaacagaca cagtaaatcg
    19981 ccaaccacgg gcatccagct taataatagc gtgactgcac catagcgttt cagccagccg
    20041 gtagctttct cttgccagcg cgatgtttta cgcaatggac agaaacgccc aaggataacg
    20101 ttagttaacc ctccaaggct attacccatt gttgctgtta aaactaaaac ccagggatga
    20161 ctgatcccag aaagcaacat tgccaccagc acgacttcgg agttgccggg taatagtgta
    20221 gcgctgagaa aactactggc aaacaacgag aaaagcgata acgcttcact cacagcaagc
    20281 gaacatccac agcgtccatg cctgctgcac gggccgcctg aataccgaaa tcggcatctt
    20341 caaagaccac acactgcgtc ggttgcacgc ccatacgctg cgcgcacaac aaaaatgtgt
    20401 ctggcgcggg tttatggtgt ttgacgtgat cggcagcgac gacggcgtca aaataacggc
    20461 gtaatcccag gtgagccagc aatgcctcag cgatggcgct ttcactcccc gttcctacag
    20521 ccattgggcg acgaccatgc caacttttca ccacttcaac aagaggaagc ggctcgacac
    20581 tatccagcag catacttctt actgcttctg ttttttcacg cgctaacgca tgcgggtcga
    20641 gatcggcctg attcagctca ataattgcct gagcaatacg ccaggtgggc gatccattaa
    20701 gcgcaatcat cgcctgaaca tcgtactgaa gaccgtagtg ccctaatact tcgcgccacg
    20761 ctttacggtg cgtaggctcc gtatccagga ttgtgccatc catatcaaaa attaaacctg
    20821 cataacgctc gtacatggtc ttctcgcaag tcgaaaaata tgacgctact ttagcgtaat
    20881 tgcttgattt tgtcgctgat ggcgaaggag aagaaaattc aggaaatgga taaagtagtt
    20941 atttagaaat gagatatttt tgaaggaaat tttttggaga agatggtgca tccgggagga
    21001 tgactcactg gcgttcgccc ttcgggccgt tgctaaagca acgttatcct tcctggtgcc
    21061 tgcggttaat tccgctacct cttcgatatc ttcattgctg aagatttcgg agaatatggt
    21121 gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct
    21181 gagctacgga tgcatcggga aaatttattt tactgctgat attgatatcg ctactaaagc
    21241 catatcaaat aagaaaatcc atcttaacca tcatcactga tgattacgga gaatatggtg
    21301 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    21361 agctacggat gcatcgggaa atttatttta ctgctgatat tgataccgct actaaagctg
    21421 tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
    21481 cgagtactct atccagctga gctacggatg catcgggaaa cttactttac tgcagatttt
    21541 ttgataccgc tactaaagcc gtatcaagta agagatggtg catccgggag gattcgaacc
    21601 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcaaatggcg
    21661 gtgaggcggg gattcgaacc ccggatgcag cttttgaccg catactccct tagcagggga
    21721 gcgccttcag cctctcggcc acctcaccac acgcctctta cgagtgcttc gaagaacttg
    21781 tttatcgctc atcgtcgctg cgtggcgcac atattacttt ctgagactta taagtcaaac
    21841 aatttttccc acacttttat cgtttgcaca cttcacgctc aattagtctg taaaaacgtc
    21901 aaaaagagtg ttttatctac agaaaaacgg aggtctggca gatagcagta atgcaaaaaa
    21961 atggagactt aagttgaatg aacgggagta aagcgaaaag actatagagt gaaggagaaa
    22021 ttttgagggt gcgtctcacc gataaagatg agacgcggaa agattagtaa ctggactgct
    22081 gggatttttc agcctggata cgctggtaga tctcttcacg gtgaacagaa acttccttcg
    22141 gggcatttac gccaatacgt acctggttgc cctttacccc taaaactgtc acggtgacct
    22201 catccccaat catgagggtc tcaccaactc gacgagtcag aatcagcatt ctttgctcct
    22261 tgaaagatta aaagagtcgg gtctctctgt atcccggcat tatccatcat ataacgccaa
    22321 aaagtaagca atgacaaaca cattacatgt aagcagtcat ggcattacat tctgttaaac
    22381 ctaagtttag ccgatataca caacttcaac ctgactttat cgttgtcgat agcgttaatg
    22441 cgaatgccgt gaagcgagtc cacggcattg cctgacgctt atattattgc aatttcgcgc
    22501 tgacccagcc tttcacactg gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg
    22561 cttgcgccat gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg accataccaa
    22621 tcagttcccc tgctttcaca cgatctgtga cgtccttaga tacgcctgca atcagagaaa
    22681 ccttaccttc ggctaccgtt gccagcacga taattgtcga ccccagctga ttttttaaat
    22741 cgtcaaccat ggtacgcaac attttcggct caacaccgct aagctcgcta accaacagct
    22801 taacaccatt aacatcaatt gccttactgg aaagatttgc gctctcctgt gcggcagctt
    22861 gttctttaag ctgttgtagt tctttttcta actgacgcgt acgttccagt actgagcgca
    22921 ctttatccgc cagattattg ctatcgcctt tcagcagatg cgcgacttcg cttaagcgat
    22981 cgctgtctgc atgaacggtg gtgatagcgc cttctccggt taccgcttcg atacgacgaa
    23041 cgcctgcagc agtacccgat tcagagatga tgcggaacag accaatatca ccagtgcggc
    23101 tggcgtgagt accgccacac agctcggtgg agaaatcgcc catgctcagc acgcgtacac
    23161 gctcatcata cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat
    23221 ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc aggtcttcga
    23281 ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag cgcagcacct
    23341 tgtcgttaac cagtgaacct ttctgcgata catgagtccc cagaacctgg cgcagcgcag
    23401 cgtgcatcag gtgcgttgcg gagtgattca aacgaatacg ggcgcgacga gcctcatcaa
    23461 catcagcctg cacagcgtcg cccactttca gagaacccgc agcaagttta ccgatgtgac
    23521 caatcgcctg gccgtatttc tgcgtatctt ccaccacgaa ggagaagtta gcgcctttca
    23581 gttcgccttt atcaccaacc tgaccgccgg attccgcata gaatggcgtt tgatccagca
    23641 cgaccacagc ttcctggcct gcattgatgg catcaaccgc tttaccatca acaaacagcg
    23701 cggtcacttt gccgttcagt tccagatggt catagccttt aaattcagat gcactgtcaa
    23761 cacggatcat tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcc cgacgacgct
    23821 gctcttccat tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa
    23881 catcagccgt caggtcaacc gggaagccat aggtgtcgta cagacggaaa gcagtttcac
    23941 catccagcgt atcaccagaa agttttgcca gctcttcatc cagcaacgcc agaccgcgct
    24001 ccagagtacg agcaaactgc tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct
    24061 ggcgtttcag gtcttcaccc gcagagccca taacgtcgat cagcggacca accagtttgt
    24121 agaagaaggt ttctttcgcg ccgagcatat taccgtgacg cactgcgcga cgaatgatac
    24181 gacgcagtac ataaccacgg ttttcattgg acggcattac gccatccgcg atcaggaacg
    24241 cacaagaacg aatgtggtca gcgattacgc gcagcgattt attgctcaga tcggttgcgc
    24301 cagtgacttt cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca tagttagagt
    24361 taacgtgttg cagcaccgca gcaatacgct ccagacccat accggtatct acagacggct
    24421 tcggcagcgg ttccatcgtg ccatcggcct ggcggttgaa ctgcatgaag acgatgttcc
    24481 agatctcaat gtagcggtcg ccgtcttctt ccggacttcc cggagggcca ccccaaatgt
    24541 ggtcgccgtg atcgtagaag atttcggtgc acgggccgca cggaccagtg tcacccatct
    24601 gccagaagtt gtcagatgcg tatggcgcac ctttgttatc gccgatgcga ataatacgtt
    24661 cgcgcgggat ccctacttct ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga
    24721 cggtaaccca cagacgctct ttcggcaggg caaaccattt ttcgctggtc agcagttccc
    24781 atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg cccagcattt
    24841 cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc caggtcgttg tgtttaccac
    24901 ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata attacgcttg tcgagcccaa
    24961 ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat
    25021 ggggtaccag ggagctgctg gcaactacct gatgtccctt actatggaaa aagtcgagaa
    25081 acgcctgacg gatctcagcg gtgctcttgc tcataattat cctgaaatca agctaacgaa
    25141 atatcgccac cagctccagc gtgtcttaac cgccgggctg gtaactgaaa agtgggaata
    25201 agataagttt tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc
    25261 caaatatcct ggatatcttc catcagatag ccacgataga gcagaaaacg ctggatctta
    25321 actttttctg aaaagacagt tggcaaaggt tcaccatatt ttcgcgtcgc ctgatcgcgc
    25381 gccagtgcgc accagtcgat gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa
    25441 atacctttct gattcagttc ctggcgaata cgcgcaggtc cataaccttt gcggctacgg
    25501 ctggcgataa agcgcgcaac aaatcggctg tcatcgagat agccatgttc atggcaccag
    25561 gcaataacgc gctcgtaatc ttctgccgta gcatcaatct cttctgggcc atttttgccc
    25621 ataatcggtg ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga
    25681 atgcgtaccg cacgatccaa caggcgagca tatgccgggc gacgggacgt tgattctgtc
    25741 atggcatatc cttacaactt aaaaagcaaa agggccgcag atgcgaccct tgtgtatcaa
    25801 acaagacgat taaaaatctt cgttagtttc tgctacgcct tcgctatcat ctacagagaa
    25861 atccggcgtt gagttcgggt tgctcagcag caactcacgt actttcttct cgatctcttt
    25921 cgcggtttcc gggttatctt tcagccaggc agttgcattc gctttaccct gaccgatctt
    25981 ctcacctttg tagctgtacc acgcgcctgc tttctcgatc agcttctctt ttacgcccag
    26041 gtcaaccagt tcgccgtaga agttgatacc ttcgccgtag aggatctgga attcagcctg
    26101 tttaaacggc gcagcgattt tgttcttcac cactttcacg cgggtttcgc tacccaccac
    26161 gttttcgccc tctttcaccg cgccgatacg acggatgtcg agacgaacag aggcgtagaa
    26221 tttcagcgcg ttaccaccgg tagtggtttc cgggttaccg aacatcacac caattttcat
    26281 acggatctgg ttgatgaaga tcagcagcgt gttggactgc ttcaggttac ccgccagctt
    26341 acgcatcgcc tggctcatca tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc
    26401 ttcgatttcc gctttcggcg tcagtgccgc cacggagtca acgacgataa cgtctactgc
    26461 gccagaacgc gccagggcgt cacagatttc cagtgcctgc tcgccggtgt ccggctggga
    26521 gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg tagattgggt ccagcgcgtg
    26581 ttcagcatcg ataaacgcac aggttttacc ttcacgctgc gctgcggcaa tcacctgcaa
    26641 cgtcagcgtg gttttaccgg aagattccgg tccgtagatt tcgacgatac ggcccatcgg
    26701 cagaccacct gccccaagcg cgatatccag tgaaagcgaa ccggtagaga tggtttccac
    26761 atccatggaa cggtcttcac ccaggcgcat gatggagcct ttaccaaatt gtttctcaat
    26821 ctggcccagt gctgccgcca acgctttctg tttgttttcg tcgatagcca tttttactcc
    26881 tgtcatgccg ggtaataccg gatagtcaat atgttctgtt gaagcaatta tactgtatgc
    26941 tcatacagta tcaagtgttt tgtagaaatt gttgccacaa ggtctgcaat gcatacgcag
    27001 tagcctgacg acgcaccgca tcacggtcgc cgctgaagca ttcccgcagg gtaatgcctt
    27061 caccgcgggc agtggcaaaa gcaaaccaga cggtgccgac aggcttctct tcactgccgc
    27121 catccggccc ggcgatacca ctaatagaca cggcataatc agcacgagcc gctttcagtg
    27181 cacctatcgc catttccacc acgacgggtt cactcaccgc gccatgctgc gccagcgtct
    27241 cttcgcgtac gccgatcatt tgcgctttgg cttcgttact gtaggtgaca aatccgcgtt
    27301 caaaccaggc ggaactgccg gcaatatcgg taatcacttt cgctacccaa ccgccggtac
    27361 aagactcagc agttgttacg gttgcgccac gggctttcag cgcctgccca acctgttcgc
    27421 ttaactgcat cagttcactg tcagtcatca caacctctgt cagtcaaaaa tttatgccgg
    27481 acaagatagc actttcgtca ggaagatggt gttggcgttg caattttacg aggaggattc
    27541 agaaaaaagc tgattagcca gagggaagct cacgcccccc tcttgcaaat agttactgca
    27601 cgcgcgccag cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtactgt
    27661 ggttataacg ggtaatggtg tagaagttcg gcagaccgta ccagtactgg taacctgttc
    27721 caacatccag acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta
    27781 aacctgccgc ggcaagctgc gaaatgctgt acctggtttt gaagccattt ggcaagcctg
    27841 gagcctgacc gtttgccatt accgcgacct gatcgccttt cacccagccg tgcgctttga
    27901 aataattcgc cacgctaccg atcgcatcaa ccggatccca caggttgata tgcccgtcgc
    27961 cgctgaaatc taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg
    28021 ccccggcaaa ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca
    28081 ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg tgggtagtta aatgacagcg
    28141 ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc
    28201 cgataatccc gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg
    28261 catcttcata ctgattccag aaaaccacac cgttctgcac attgtccggc gtaataaatt
    28321 ttttgcgata acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg
    28381 gtgcctggtt atccatcagc cgcagtaccg aatccagacg tttcgcctgg gagagaattt
    28441 cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat tctgtcgatg aactgctggg
    28501 cattcgggtt attagcgaaa tcgccgccca tctgcatcac attgtgctgc ggctcaagca
    28561 ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc
    28621 tacaggcggc aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga
    28681 ggggtccatt taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa
    28741 ggaagcggta gtcactgccc gatacggact ttacataact caactcattc tcctcgctat
    28801 ccttttactc aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac
    28861 tttaaatctt tcaatctgat tagattaggt tgccgtttgg taataaaaca ataaatcctg
    28921 aaggagagaa caatgataga aaccattact catggtgcag agtggtttat cgggctgttc
    28981 caaaagggcg gagaggtgtt taccgggatg gtgaccggca ttcttccgct gttgattagc
    29041 ctgctggtta tcatgaacgc actgattaat tttatcggtc agcatcgtat tgaacgtttt
    29101 gctcaacgtt gcgccggtaa ccctgtttcc cgttacctac tgttaccgtg cattggcacg
    29161 tttgtctttt gcaatccgat gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa
    29221 cccagctact acgcggcggc ctcttatagc tgccactcaa tgaatggcct cttcccccat
    29281 atcaaccctg gcgaactgtt tgtttatctt ggcattgcca gcggtctgac aacgctgaac
    29341 ctgccacttg gcccactggc ggtgagctat ctgctggttg gtctggtcac caacttcttc
    29401 cgcggctggg tgaccgatct gaccaccgct atttttgaga aaaagatggg cattcaactt
    29461 gaacaaaaag ttcatctggc aggagcaaca tcatgacgca tattcggatc gaaaaaggaa
    29521 cggggggctg gggcggcccg cttgagctgg aaaccacgcc gggcaaaaaa atcgtctata
    29581 tcaccccgcc ggtacccggc ctgcgattgt cgacaaactg gcacagctta ctggctggca
    29641 ggctattgac ggatttaaag aaggtaaacc agcggaggcg gaaattggtg tcgcggtaat
    29701 cgactgtggc ggcacattac gctgcggcat ttatccgaaa cggcgtattc ccaccattaa
    29761 tatccactcg acgggcaagt ccggtccact ggcgcagtac attgtggaag atatttatgt
    29821 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
    29881 cgtgggccgt gactatgaca ccagcaagaa aatcaccgaa caaagcgatg gtttactggc
    29941 gaaggtggga atgggtatgg gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac
    30001 catcgacact gtattaaaaa ccattcttcc gtttatggca ttcgtctcgg cgctcattgg
    30061 catcattatg gcttctggcc ttggtgactg gattgcccac ggccttgctc cgctggcgag
    30121 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccgctgc tttcaccttt
    30181 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
    30241 tctcggcaat attccgccgc atctggcttt acctgcactg tttgccatca acgcgcaggc
    30301 ggcctgcgac ttcattccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
    30361 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
    30421 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
    30481 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
    30541 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
    30601 gcgagttgaa aggtgcactc catcccggtt tgcaatttac gctcgggcag catcgctatc
    30661 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
    30721 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
    30781 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
    30841 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
    30901 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
    30961 atgtggcaca agaaattaac gccgaatatg gtgaaggaac agcgtatggt tttggtgctg
    31021 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttgttc
    31081 gtgtggattt actggtctac agcgccggaa ttgcaaaagc agcctttatc agcgacttcc
    31141 agctcggcga ttttgaccgt tcgctacagg tgaatctggt gggttatttc ctgtgtgcgc
    31201 gtgaattttc gcgtttgatg atccgcgatg ggattcaggg acgcattatt cagatcaact
    31261 cgaaatccgg caaagtgggc agcaaacaca attctggtta tagcgccgcg aaatttggtg
    31321 gcgtagggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
    31381 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcgctgttg ccacaatatg
    31441 ccaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    31501 tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct gctgttctac gccagtccta
    31561 aggcgtcgta ctgtaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    31621 caacagcgga gatccgttaa ggatctccgt gagactaata gaatgcctga tgcgctacgc
    31681 tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca
    31741 tcactgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc
    31801 gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca
    31861 gcgggcgctt taaaccgcgg gtcgtggtcg ccatcgccct ggacgatcag cagcgcatcg
    31921 ccgacacctt gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa
    31981 ttaccggtat gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat
    32041 cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt
    32101 acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc
    32161 gtcagcgtca agccgccatt ctggagtatc tgcaaaagca gggaaaatgc tcggttgaag
    32221 aattggcgca atattttgac accacaggca caactattcg caaagatctg gtcattctgg
    32281 aacatgccgg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg
    32341 atccgcctat cgatcataaa acactcatca acacccacaa gaaagagctg atagcagaag
    32401 ctgccgttgg ttttattcat gatggcgatt cgatcattct tgatgctggc agtaccgttt
    32461 tgcagatggt gcctctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc
    32521 atatcgtcaa tgcgttatct gaactggata atgaacaaac tatcctgatg ccaggcggaa
    32581 cgtttcgtaa aaaatcggcc tcatttcacg ggcagctggc agagaatgcc ttcgagcatt
    32641 tcaccttcga caaattattc atgggtaccg acggcatcga tctcaatgcg ggcgtaacca
    32701 ccttcaacga ggtctatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt
    32761 tgatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa
    32821 gcgtcgataa gctgattacc gacgcagata tcgatccggc gtttcgtcag gcgctggaag
    32881 agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg
    32941 ggacgtcaga cgttaatgct ggaattgcag gaagcaagcc gtttaccgga acgtctgggc
    33001 gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg
    33061 ggaattggca aatcgggcca cattggtaag aaaatcgccg caacgcttgc cagtaccggc
    33121 acaccggctt tttttgtcca tccggcagaa gcgctgcatg gcgatctggg gatgatcgaa
    33181 agccgcgatg tgatgctgtt tatctcttac tccggtgggg cgaaggaact ggatctgatt
    33241 attccgcgtc tggaagacaa atctatcgcg ctgctggcga tgaccggcaa accgacgtca
    33301 ccgctgggcc tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg
    33361 atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg
    33421 atggcggtca tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc ccaccctgct
    33481 ggagcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg tgacgatgcc
    33541 atcccacagg tggcgttaac cgccagcgtg atggatgcta tgctggaact cagccgcacc
    33601 ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac
    33661 ggcgatttac gtcgctggct ggttggcggc ggcgcactca ccacgccagt caatgaagcg
    33721 atgacggtcg gcggcaccac gttgcaatcg caaagtcgcg ccatcgacgc caaagagatc
    33781 ctgatgaagc gcaaaatcac tgccgcaccg gtggtggatg aaaacggcaa actcaccggc
    33841 gcaataaacc tgcaggattt ctatcaggcc gggattattt aatccttcaa tcccagacgt
    33901 ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca tccgcgcgca ggcagcccag
    33961 ttgtgatgat tttgtgccag tgcctgacga atagtttcac gctggaacgc ttctgtcgct
    34021 tcacgcaggt tttgcttaac aacgggcacc gccgccactt ctggcggcgg caacgtcacc
    34081 tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgctgcg ggtggctctc
    34141 gccagcacta ccgcccgatg aatagcatgt tccagttcgc gcacgtttcc cggaaaactg
    34201 tagtgttgca gtaaatttcg cgccccggca cttaatacca cgcgggagag cccctgccgc
    34261 aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc
    34321 agcggcggca ccgaaagtgg aaacacactc aggcgatgaa acaaatcggc gcggaatcgc
    34381 cctgccagca cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc
    34441 cgcaaactac ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc
    34501 ttggcctgca atgccaacga caactcgccg atctcatcga gaaacagcgt gccgttatcc
    34561 gccatttcaa acttcccact gcggttactg atagcgccag taaacgctcc tttcacatgc
    34621 ccgaacaact cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc
    34681 ggattcaccg cccgtggcga ggcctcatga atcgctttcg ccaccagctc cttaccggtt
    34741 cccgtctcac cgctgatcag gacgttgaga tcggacgccg ccacaatctc aatctctttt
    34801 ttcagttgcg tcatgcctgg cgacagaccg atcatctgcg tctgtttcac cgcttcaaat
    34861 ggagcggcat cgcccggcag catattctgg ctttccagtt gttcaatcag caacgcattg
    34921 cttaacgctc ccgccgccag cgcggcaatc aggcgtaact cttcgtcgct gaaaacatcg
    34981 aactgatcgg gctgcatccc gtcgagcgtc aatgcgccga tcaggttttg cccggcaaac
    35041 agcggcagac caacgcaggc gtgaaccttc agactctcct gccctggaat caaaccgtca
    35101 tagggatcgg gcaattcgct gtctgcggga aagcgcacca catcacccgc gcgggcaatc
    35161 gcttccagcc gtggatgacc ttccagcgca aagcgtctac cgagtacatc cttcgccagc
    35221 ccgtcgatgg caagcggaat aaactgccgc gagtcgtaac gtagcaacgc agacgcatcg
    35281 cactccagca cctgacgcag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc
    35341 ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc
    35401 tcactgtcaa tttgactata gatattgtca tatcgaccat tggattgata gtcattttga
    35461 ctactcatta atgagcataa ttttatttat agagtaaaaa caatcagata aaaaactggc
    35521 acgcaatctg caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta
    35581 tgtctattgt ggtgaaaaat aacattcatt gggttggtca acgtgactgg gaagtacgag
    35641 attttcacgg cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc
    35701 gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa attcagccgc gaatttgtgc
    35761 agaacctgcg taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgcag
    35821 aagaggacca cgccggggcg ctgaccgaac tgatggcaca aattcccgat acgccgatct
    35881 actgtacagc caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt
    35941 ttaatgtggt gaaaactggc gacacgctgg atatcggcaa cggcaaacag ctcatttttg
    36001 tcgaaacacc aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg
    36061 tgctgttcag taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg
    36121 aagtggatca gacggagctt tttgagcagt gccagcgtta ctacgccaat atccttacgc
    36181 cgttcagccg tctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg
    36241 atatgatagc gacctctcac ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc
    36301 tgtacctgaa atgggcggct gattatcagg aagacagaat caccattttc tacgacacca
    36361 tgtcgaataa cacccgcatg atggctgacg ctatcgccca ggggattgcg gaaaccgacc
    36421 cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca
    36481 atgtcttccg ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac gtgatgatgc
    36541 cgaaaatcgc cgggctggtg gaggagatga ccggattacg cttccgtaac aaacgcgcca
    36601 gtgctttcgg ctctcacggc tggagcggcg gtgcggtgga tcgtctttcc acgcgcctgc
    36661 aggatgcggg tttcgaaatg tcgcttagcc tgaaagcgaa atggcgacca gaccaggacg
    36721 ctctggagtt atgccgtgaa cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc
    36781 tgccgcagag cacggtgaat acggtagtta aagaagaaac ctctgccacc acgacggctg
    36841 acctcggccc acggatgcag tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg
    36901 agccaatgca ggacgttgcg ccaggaacgc cgtggagtga agtcccggat aacttcctgt
    36961 gcccggaatg ctccctcggc aaagacgtct ttgacgaact ggcatcggag gcaaaatgag
    37021 caacggcatt gtgatcattg gttcgggctt cgccgcccgc caactggtga aaaatattcg
    37081 caaacaggac gccagtattc cattaaccct gattgccgcc gacagtatgg atgagtacaa
    37141 caaacctgac ctcagccatg ttatcagtca ggggcaacgt gccgacgacc ttacccgcca
    37201 gacggcgggt gaatttgccg agcagtttaa tctgctcctg tttccgcaaa cctgggtgac
    37261 ggatatcgat gccgaagccc gtgtggtgaa aagccagaat aatcagtggc aatacgacaa
    37321 gctggtgctg gcaaccggtg ccagcgcctt tgtcccgcca gtgcctgggc gtgagttaat
    37381 gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg
    37441 acgcgtgttg attgttggcg gtggtttgat tggtagcgaa ctggcgatgg atttttgtcg
    37501 tgcaggcaaa atggttacgc taatcgacaa cgctgccagt attctggcgt cgttaatgcc
    37561 accggaagta agcagtcgct tgcagcatcg gttgacggag atgggcgttc atctgctgtt
    37621 gaaatctcag ttgcaggggc tggaaaaaac ggattctggc attctggcaa cgctggacca
    37681 ccagcgcagc atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc cggaaaccgc
    37741 cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca
    37801 aaccagtaat gccgatattt acgcgctggg cgattgcgcg gaaattaacg gtcaggtatt
    37861 gccgttcctg cagccgattc aacttagcgc gatggtgctg gcaaaaaatc ttctcggcaa
    37921 taacacgccg ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg aattaccgct
    37981 gcatctggca ggcgaaaccc agcgtcagga tttacgctgg cagattaata ccgaacgcca
    38041 gggaatggtg gcgcgcggtg ttgacgatgc agaccaactt cgcgcctttg tggtcagtga
    38101 ggatcggatg aaagaggcat ttggattgtt gaaaacgttg ccggtgtagg tgggctactg
    38161 tgcctaaaat gtcggatgcg acgctggcgc gtcttatccg acctacgggg acgcatgtgt
    38221 aggccggata aggcgtttac gccgcatccg gcaatggtgt ccaaatgcaa cacgttttat
    38281 ccgttctgga cttcacccgc taaccaacgc gccgcagcaa taaccccctg ccccagagac
    38341 aaaccgccat cacccgccgg taaactctgt ggaaacagca atgtgaaatc agcgagataa
    38401 tgcgccagac gtgcacgcag caaacggtta tgaataaccc cgccgctaaa taccagcgta
    38461 gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg cgccagcgca
    38521 tcatgaaacg cccacgcgcg ttgattaacc ggtgcctgcc agttcagcca ctgctgccag
    38581 aaagtggcga gatccagttg attgtccacc cgcggcatcg tcaccggatg cgtcactccg
    38641 tcgcatgagg ctgctagcgc ctccagagca caagccgctt caccttcata acttaacgtg
    38701 gctggcgcac agcccagtgc cgccgccaca gcatcgaaca aacgcccaca cgatgacgcc
    38761 agcggcgcgt taattccacg ctcaatggcc cgcgccagta cgctccagtt ttgctgttgc
    38821 acacttgccg tttcagggta attctgccac tccggcacaa agcgcaggca ctgcgccagc
    38881 aggttacgcc acggctgctt cgccgccaaa tcgccacccg gaagcgccac tgcaggcaag
    38941 ccgcccaggt gctcacattc gcgatagttc acccgcaggc actcgccgcc ccacaaagcg
    39001 ccgttctccc ccataccgat accgtcgagc gtcaaagcaa tgacatcacc gccatccagc
    39061 ggccactgat gctctgccag acacgccgct gcgtgggcat gatgatgcag caccgtttgc
    39121 gtcggcagat tcatttcgct cgcccactgg caggagacat agcccggatg tgcgtcatgc
    39181 acaacgtatt gcggagtgaa attgtagatg ttttgcatca ggcgtaacgc ttcgcgccac
    39241 tgcgtctgga tgccatcgtc acttaaatcg cccagatgct ggctcaacac cacttgttca
    39301 ccgcgcacca ggcagaaggt atttttcaga tccgcgccga gacacagtac aggcggaaca
    39361 tttttaaagc ccggaggcaa agccagcgca tccggcacat acccccgcga acggcgcagc
    39421 atttcgccgc tttcgcgcac caccgaatca tccatccgct gcacaatgtc gcggttgtgt
    39481 attaagaatc cgtcggcaat gccctgcaaa tcctccagcg cctgttcgtt gctgatagcc
    39541 ggtggtttac cgctcaggtt gccagaggtc atcaccagcg ggcattgtag ttcctgtaac
    39601 agcaaatgct gaagcggatt cgcaggcaac attaccccga cttcgttaag gccaggggcg
    39661 atatcatcac aaagctcagg aacgtatttt ttatccacca gcacaatcgg cgcggcgggc
    39721 gtggtaagca actggcgcgc agcgtctggt aaaccgtcag ccactggcaa catgactgcc
    39781 agcggtttcg ccgggcgatg tttgcgcgcc cgaagtgtcg ccaccgcgtt actgttacgt
    39841 gcatcgcagg caagatgaaa tccgccaatc cctttgatgg cgacaatgtt gcccattttt
    39901 aactgtgcga tagccgcctg taatgccgcc tcttgttctg catgttcacc atgacttacc
    39961 cattcaagat acgggccaca ctccgggcag gccaccggct gggcgtggaa gcgacgatcg
    40021 agcgggtcgc ggtactcttt atcacaggct ggacacagcg gaaacgccgc catcacggta
    40081 aacgggcgat cgtaaggcat ggcgcgaata atggtgaagc gcgggccgca gtgggtacag
    40141 ttgataaatg gataacgata acgccgttcg cctggggtat tcatttcggc aaggcaagca
    40201 gggcaagtgg cggcatcggg aacaatttgc gtattcatgg cgccgcccgc gctctggcgg
    40261 atggtgaact cagtgggcag ttgtgaccag ataaacggtt cacgctcaac gctatcaata
    40321 cgcgccagcg gcgggcagtg ctgatgcaat tgaacaagaa acgtttccgg gtcttccagc
    40381 agccggactt ctacgccatc gccgtcatta cagacatcgc cgtgaagatt taattgctgt
    40441 gccagctgcc agacaaacgg acgaaaaccg acgccctgca ctttgcctcg aatacgcagt
    40501 tggacaccgc aagatgtgtt ttttgccatt gagttattcc cgccatcatg aattgcgtaa
    40561 cccgtccagc cggacacgac agcgtcgcat ccggcagtca caggtcggcg ataccgcccg
    40621 ctccgtattc tacgaatatt tccgggaatt cctttgatgc cagaacagtt ctgtaagatt
    40681 tttagaacat cagcgccgta cggcggcgtt tttctgcgct cagttgttca agtttattgc
    40741 gatcgacaca aattagcgca tgggtcgggc aagccgccat acacgccggg ccgtcttcac
    40801 gatggttgca caggtcgcat ttattggctt cggctttgtc agcccgtaca ttcagccctg
    40861 cgccgctgtt gcgaatcacc ggacgtacca ccacttccat cgcgccatac gggcaagcca
    40921 caacgcaggt tttgcaacca atgcaacgtt cctgcatcac atgaacaaac cctttatcac
    40981 ggctgatagc accattcggg cagacgttag cgcacggtgc atcttcacac tgacggcaaa
    41041 ctgtcgccgt ggaaatgttc acacctttaa tgacatggat acgcggtaaa aatgtttccg
    41101 gggtcagcga tgcacagtcc tgattttcct gatgagaaac cacgcacgcc acttcacagg
    41161 tacggcaacc aatacattta ctcgcgtcag caatgatgaa acggttcatc aaattctcca
    41221 gcaatgacag ttaatgcgcc gatacattca caaatcatgc cagtttttaa ttt
//
LOCUS       DAZGXS010000025        41219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGXS010000025 DAZGXS010000000
VERSION     DAZGXS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(171..434)
                     /gene="cedA"
                     /locus_tag="ACHKG5_001488"
     CDS             complement(171..434)
                     /gene="cedA"
                     /locus_tag="ACHKG5_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0867613.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            617..2878
                     /gene="katE"
                     /locus_tag="ACHKG5_001489"
     CDS             617..2878
                     /gene="katE"
                     /locus_tag="ACHKG5_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0867614.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2925..3683)
                     /gene="chbG"
                     /locus_tag="ACHKG5_001490"
     CDS             complement(2925..3683)
                     /gene="chbG"
                     /locus_tag="ACHKG5_001490"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0867615.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3696..5048)
                     /gene="celF"
                     /locus_tag="ACHKG5_001491"
                     /gene_synonym="chbF"
     CDS             complement(3696..5048)
                     /gene="celF"
                     /locus_tag="ACHKG5_001491"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0867616.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5154..5996)
                     /gene="chbR"
                     /locus_tag="ACHKG5_001492"
     CDS             complement(5154..5996)
                     /gene="chbR"
                     /locus_tag="ACHKG5_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0867617.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6004..6354)
                     /gene="chbA"
                     /locus_tag="ACHKG5_001493"
     CDS             complement(6004..6354)
                     /gene="chbA"
                     /locus_tag="ACHKG5_001493"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0867618.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6405..7763)
                     /gene="chbC"
                     /locus_tag="ACHKG5_001494"
     CDS             complement(6405..7763)
                     /gene="chbC"
                     /locus_tag="ACHKG5_001494"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0867619.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7848..8168)
                     /gene="chbB"
                     /locus_tag="ACHKG5_001495"
     CDS             complement(7848..8168)
                     /gene="chbB"
                     /locus_tag="ACHKG5_001495"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0867620.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8468..8806)
                     /gene="osmE"
                     /locus_tag="ACHKG5_001496"
     CDS             complement(8468..8806)
                     /gene="osmE"
                     /locus_tag="ACHKG5_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0867621.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9008..9835
                     /gene="nadE"
                     /locus_tag="ACHKG5_001497"
     CDS             9008..9835
                     /gene="nadE"
                     /locus_tag="ACHKG5_001497"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0867622.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10065..10952
                     /gene="cho"
                     /locus_tag="ACHKG5_001498"
     CDS             10065..10952
                     /gene="cho"
                     /locus_tag="ACHKG5_001498"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0867623.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACYGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10912..11487)
                     /gene="ves"
                     /locus_tag="ACHKG5_001499"
     CDS             complement(10912..11487)
                     /gene="ves"
                     /locus_tag="ACHKG5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0867624.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11690..12175)
                     /gene="spy"
                     /locus_tag="ACHKG5_001500"
     CDS             complement(11690..12175)
                     /gene="spy"
                     /locus_tag="ACHKG5_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0867625.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12505..13473)
                     /gene="astE"
                     /locus_tag="ACHKG5_001501"
     CDS             complement(12505..13473)
                     /gene="astE"
                     /locus_tag="ACHKG5_001501"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0867626.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13466..14809)
                     /gene="astB"
                     /locus_tag="ACHKG5_001502"
     CDS             complement(13466..14809)
                     /gene="astB"
                     /locus_tag="ACHKG5_001502"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0867627.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14806..16284)
                     /gene="astD"
                     /locus_tag="ACHKG5_001503"
     CDS             complement(14806..16284)
                     /gene="astD"
                     /locus_tag="ACHKG5_001503"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0867628.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16281..17315)
                     /gene="astA"
                     /locus_tag="ACHKG5_001504"
     CDS             complement(16281..17315)
                     /gene="astA"
                     /locus_tag="ACHKG5_001504"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0867629.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17312..18532)
                     /gene="astC"
                     /locus_tag="ACHKG5_001505"
     CDS             complement(17312..18532)
                     /gene="astC"
                     /locus_tag="ACHKG5_001505"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0867630.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            18978..19784
                     /gene="xthA"
                     /locus_tag="ACHKG5_001506"
     CDS             18978..19784
                     /gene="xthA"
                     /locus_tag="ACHKG5_001506"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0867631.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19951..20661
                     /gene="ydjX"
                     /locus_tag="ACHKG5_001507"
     CDS             19951..20661
                     /gene="ydjX"
                     /locus_tag="ACHKG5_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0867632.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20666..21343
                     /gene="ydjY"
                     /locus_tag="ACHKG5_001508"
     CDS             20666..21343
                     /gene="ydjY"
                     /locus_tag="ACHKG5_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0867633.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            21358..22065
                     /gene="ydjZ"
                     /locus_tag="ACHKG5_001509"
     CDS             21358..22065
                     /gene="ydjZ"
                     /locus_tag="ACHKG5_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP0867634.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22065..22613
                     /gene="ynjA"
                     /locus_tag="ACHKG5_001510"
     CDS             22065..22613
                     /gene="ynjA"
                     /locus_tag="ACHKG5_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0867635.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22623..23789
                     /gene="ynjB"
                     /locus_tag="ACHKG5_001511"
     CDS             22623..23789
                     /gene="ynjB"
                     /locus_tag="ACHKG5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0867636.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23762..25297
                     /gene="ynjC"
                     /locus_tag="ACHKG5_001512"
     CDS             23762..25297
                     /gene="ynjC"
                     /locus_tag="ACHKG5_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0867637.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25297..25950
                     /gene="ynjD"
                     /locus_tag="ACHKG5_001513"
     CDS             25297..25950
                     /gene="ynjD"
                     /locus_tag="ACHKG5_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0867638.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALARQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26017..27324
                     /gene="ynjE"
                     /locus_tag="ACHKG5_001514"
     CDS             26017..27324
                     /gene="ynjE"
                     /locus_tag="ACHKG5_001514"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0867639.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27333..27953)
                     /gene="ynjF"
                     /locus_tag="ACHKG5_001515"
     CDS             complement(27333..27953)
                     /gene="ynjF"
                     /locus_tag="ACHKG5_001515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0867640.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28040..28447
                     /gene="nudG"
                     /locus_tag="ACHKG5_001516"
     CDS             28040..28447
                     /gene="nudG"
                     /locus_tag="ACHKG5_001516"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0867641.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28413..28685)
                     /gene="ynjH"
                     /locus_tag="ACHKG5_001517"
     CDS             complement(28413..28685)
                     /gene="ynjH"
                     /locus_tag="ACHKG5_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP0867642.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28921..30264
                     /gene="gdhA"
                     /locus_tag="ACHKG5_001518"
     CDS             28921..30264
                     /gene="gdhA"
                     /locus_tag="ACHKG5_001518"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0867643.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30381..31421)
                     /gene="ynjI"
                     /locus_tag="ACHKG5_001519"
     CDS             complement(30381..31421)
                     /gene="ynjI"
                     /locus_tag="ACHKG5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0867644.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31549..33510)
                     /gene="topB"
                     /locus_tag="ACHKG5_001520"
     CDS             complement(31549..33510)
                     /gene="topB"
                     /locus_tag="ACHKG5_001520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0867645.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKIYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33515..34558)
                     /gene="selD"
                     /locus_tag="ACHKG5_001521"
     CDS             complement(33515..34558)
                     /gene="selD"
                     /locus_tag="ACHKG5_001521"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0867646.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34675..35226)
                     /gene="ydjA"
                     /locus_tag="ACHKG5_001522"
     CDS             complement(34675..35226)
                     /gene="ydjA"
                     /locus_tag="ACHKG5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0867647.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35387..37243
                     /gene="sppA"
                     /locus_tag="ACHKG5_001523"
     CDS             35387..37243
                     /gene="sppA"
                     /locus_tag="ACHKG5_001523"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0867648.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37359..38069
                     /locus_tag="ACHKG5_001524"
     CDS             37359..38069
                     /locus_tag="ACHKG5_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFP0867649.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            38201..39217
                     /gene="ansA"
                     /locus_tag="ACHKG5_001525"
     CDS             38201..39217
                     /gene="ansA"
                     /locus_tag="ACHKG5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0867650.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            39228..39869
                     /gene="pncA"
                     /locus_tag="ACHKG5_001526"
     CDS             39228..39869
                     /gene="pncA"
                     /locus_tag="ACHKG5_001526"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0867651.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39962..>41219)
                     /gene="ydjE"
                     /locus_tag="ACHKG5_001527"
     CDS             complement(39962..>41219)
                     /gene="ydjE"
                     /locus_tag="ACHKG5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867652.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT        10231 a        10848 c        10147 g         9993 t
ORIGIN      
        1 aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt atttatagca
       61 atagatcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag caatgaaata
      121 gagaatttca ggccatgtaa ctcccggcaa aaccgggagg tacgtaatct ttactcagtc
      181 acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg cacagtaaac
      241 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      301 atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      361 cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt cattactggc
      421 ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta gcccctgtac
      481 gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcggggtctg
      541 gctggtggtc tatagttaga gagttttttg accaaaacag cggccctttc agtaataaat
      601 taaggagacg agttcaatgt cgcaacaaaa cgaaaagaac ccacatcagc accagtcacc
      661 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aggacggctc
      721 tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgcct cagggagcct
      781 gaaagcccct gatacgcgta acgaaaaact taattctctg gaagacgtac gcaaaggcag
      841 tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgacgatc aaaactcact
      901 gcgtgccggt agccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      961 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgtggat cagccgctca
     1021 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1081 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gtgctggctc
     1141 tgccgatacc gtgcgtgata tccgtggctt tgccaccaag ttctatactg aagagggtat
     1201 ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1261 cgattttgtt catgcggtaa aaccagaacc acactgggca attccacaag ggcaaagcgc
     1321 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1381 ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct ttggtattca
     1441 taccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc
     1501 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactaaccg gacgtgaccc
     1561 ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc cggaatacga
     1621 actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg atcttctcga
     1681 tccaactaaa cttatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1741 caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1801 tattgttcct ggtctggact tcaccaacga tcccctgttg cagggacgtt tgttctccta
     1861 taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc cgattaaccg
     1921 cccaacctgc ccttaccata atttccagcg tgacggcatg catcgtatgg ggatcgacac
     1981 taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg aaacaccgcc
     2041 gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa ataaagttcg
     2101 cgagcgcagt ccatcttttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2161 gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtcgt
     2221 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2281 ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga atatcacccc
     2341 acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2401 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2461 ccttctggcc attctcaagg cgctgaaggc caaaggcgtt catgccaaac tgctctactc
     2521 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2581 cggtgcgcct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata tcgcggatat
     2641 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2701 tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2761 agaagggatt gtggaagctg acagcgccga cggtagtttt atggatgaac tgttaacgct
     2821 gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc cggcgtaaat
     2881 atggataagg tcacgtcccc tggggagtga ccttaccacc ttacttacac atcaagataa
     2941 ctacccaacc gataaccacg ctcagcaatt gcatatttca acgacgctga tgtcagcact
     3001 tccagctccg ttaaccgtgg gaaacagtaa gcactctggc gaatcgtatt atcgacaaac
     3061 gccggatggc acatgacttc cagcgagcgt tccccgcgat ggctggaatc atcaagcact
     3121 tgcaggaaca gtgcctcgct aatctcttca ccataaaatg cgctactgaa tccgtgcgaa
     3181 ctgcgcagat tagccggtaa atcaccagcg ttagatagcg gctgacggtc gatgcgcaac
     3241 gcaatcccct cttcagccgc aaacctggca acaataggga aaatctgcgg gaacatatgc
     3301 acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa atagctcaat gaaacgcaaa
     3361 tactgactgg caagctcctg agtaatttct tccagcggta aagcatcttc ttctgccaac
     3421 tgccagatcc attttcccag cacaccatcg cgggttaacc ccggcatagc tgtcagtggc
     3481 ttgcccatag taaggacaaa gtgcatccct atggccagac tcggttcatc acgactcaac
     3541 tgcaccgcat ggtcaatagc ctgcccattc accagcgccg tcgtcgatgt gacaatccca
     3601 ttgcgacagg cctcgataat gccgtagttc tgacctttgc ttaagccaaa atcatcggca
     3661 ttaacaatca gtaagcgttc cataatcagc ctcggttaat gtgctttttt aagctctgcg
     3721 atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca gaatcatctc gcgtgccagc
     3781 agctcagcat cgcgatcgga atgcaccaac ggactaaggt ttagcgccag taacacatcg
     3841 ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct cgaagccttt aatggtgtga
     3901 atcagcccca tcactttatc atcgaaatgc gtaatgcgcg gatgtggcgt cgcgccatcg
     3961 cgccccagcg tacaggtcat ttctaccgcc cagtctgccg gaatattatc aatatgccca
     4021 tgatgcggga tattaacgta atgttctgct tgcttatcgt tgtagatagc gttgatcact
     4081 tcgcacgctg catcagagta ataagcccca ccgcgctgtt ccagttcttt cggcttaact
     4141 ttcaactccg ggtttttata cagctcaaaa agttgtttct cgactttctg tactacctgc
     4201 gctcgtgcgc cgcctttgta gtattcgccc atttcaatag ccagcatctc tttttgcttg
     4261 aagtaataaa gcaaataaga acacggcaac agattcagag aacgaattaa gccttcgcta
     4321 aatggcagat cgaaaatatt tttaacgcca gatgctttta actgcccgga tgccacacca
     4381 tcaagcaatt cggcaaagcg cgacttgcca tttaccagca catccttaat gaacaccata
     4441 tggttgaggc cgaacagatc gatagataaa tcatcgctgt ctttcagcat cagaacatcg
     4501 cgaataaaca tcttcatgcc gatcggaata ttacacacgc cgataaagcg tttaaatccg
     4561 gtatgacgat aaacggcttc agtgaccatt cccgccgggt tagtgaagtt aatcacccat
     4621 gcattcggac aaagttcttc gacatctttt acgatgtcaa aaatcaccgg aatggtacgc
     4681 agacctttaa acagaccgcc cgcgccgttg gtttcctgac caagataacc atgacttaat
     4741 ggaatacgtt catccagttc acgcgccggt aattggccaa cgcgcagttg ggtagtaacg
     4801 aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt tataaagctt catcgggacg
     4861 ccagcgttat caatcatccg ttggcagaga tcgaaaataa tatccagttt agctttgcca
     4921 ccttcgacat ccaccagcca taattcgctg accggcaatt cgtgataacg cttaataaat
     4981 ccttccagta actccggggt atagctgctc ccgccaccaa tagtgacgac ttttaatttc
     5041 tggctcataa tttctccctt cagtacagaa tactgatatc tggcatatct gccccccccg
     5101 gacataaata atccagcaac aggacagata tgtgaattgt caggtataac gacttactga
     5161 ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa tttcttaaac
     5221 gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc cgtcactgaa
     5281 tagttggtca tttccagttg ttttttggca aaattaatac ggatttcatt aataatctgc
     5341 attggcgttt tgccataata tcgttgagtc gcccgcgtca aatattcctg tgatttggct
     5401 gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc atgcatcttt
     5461 tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc gcggtaatga
     5521 cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag ccctgtttcg
     5581 cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt attggtccgg
     5641 tatacctgcg aagctacaaa gcaataaggc aacaatggca ggtaatgctg ctcaaaaaag
     5701 cgtttactga tcccaacgtt caatatgcgc gtggcaccaa actcataaaa actttgatgg
     5761 tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg cttaccgtta
     5821 atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc gtgctgatgc
     5881 agtccgctga tactctcagt tttgttatag ataaacacat ggaagttttt gccattaaac
     5941 aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg catcatcgac
     6001 tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt cacgcgcaag
     6061 catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt tcatcttacc
     6121 ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat tcaatgccat
     6181 ccgtgactga tccatcatgg cttttgctgc ggcaaaatcg ccctgcttcg cctgtttcag
     6241 tgcggcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca tcaccacttc
     6301 ttccagctct tcagcttccg tttgcgtatc gggaatatta tcgagatcca tcatacatcg
     6361 ttcctctctt ttcttaccgg cacgattacc cgtaccggca tcgattaaaa tttcagggcg
     6421 ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt gttagccacc
     6481 acaacaaagg gcagataaat taacgttgcg ataccaaggt tgaagagtgc aaccagcaat
     6541 gcgacgacgc taccgttggt gttaaagaag gctcccagac cggttggcat ggtccacggt
     6601 gcaatattgg tcaccggagg aataatgccc atgtagtacg ctgccagggt gattgccgcc
     6661 agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat aattggcaga
     6721 ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag ttttgccacc
     6781 tgacgataat cagcacggcg agaggcaata aagatagcca ggatcaggcc taaagtcgca
     6841 ccactgcctc caaggaaaat aaaggagtcc agcatcggct tggcccagat atggaaggtc
     6901 ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc gatattttcc
     6961 agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc gccatgaata
     7021 ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac gctacccaac
     7081 gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt gccccaggta
     7141 ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg aattaatgcc
     7201 gagaaggaac gcgagactga agcaggtacg ctgtcgggca gtttaatgac ccaattgcgg
     7261 cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc ggagatgata
     7321 ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact atatggggtg
     7381 acggtcataa atgctgcaac ggataacaac ccagccgcca gcgcatcgac tttacgctct
     7441 tctgccagcg ccatgccaat aaagaacggt gccattaaag acattatccc taatgttccg
     7501 ttatatacgt tgccgccaat acctttcaga ccattaagtg tttcaatggt tgaggcgtcg
     7561 aggcgaatac ctaaggaata aaaaaacgac ccctccccaa agcttagaaa aacgttgtta
     7621 attaatacaa acatggcccc cgcaagggtt aacggcatta agcgaataaa gccatttttg
     7681 attgcattaa cgtgtggctg ctttcctatt ttaactgcaa aagggaggag taccttttca
     7741 agcgatgcaa taacattact catagaaaaa tacccttaaa aaccgcaatt taaatattgc
     7801 ggtattgatt tatgaaataa ctctttgacg ggaaaattta aaataaatta atttgctgcg
     7861 gcttttttaa tcgctgcaac cgcagcctta agcacgccta aaccatcgac tttgccataa
     7921 agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat ttcgggcaac
     7981 atataagcaa tctgcggccc taataacacg acatcggcat tctgaccttt ttcaccagcc
     8041 agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc ctgtgcgcgc
     8101 atttttgata ccagtaaaga ggtagacatg cccgcagaac aaaacagata aatgtgtttc
     8161 ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc gcaagcctta
     8221 acctgcttcc atgctctggg taacttgcga aaccaacatg actttttttg tggctgaaag
     8281 gagtatacgg tatagacctg agcaatagta tttctttgac cttataaatt gtctctcctt
     8341 gacctgcggt tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga taattaagaa
     8401 ctcaggccaa aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac
     8461 gaagtgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg
     8521 agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac
     8581 gttgacccag gatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg
     8641 aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca
     8701 ccacaggctg tacaaactgg tctttggtac gatcataagc cgtacaaccc gccagcatgg
     8761 ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctattg
     8821 tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc ccatgaacga
     8881 aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc
     8941 gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag
     9001 gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa
     9061 tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc aaacttatcc
     9121 gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggtaa
     9181 gctgtgccag atggcgatta atgagctgcg ccaggaaacc ggcaacgaat cactgcaatt
     9241 tattgccgta cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat
     9301 tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggctag
     9361 cgagcaggca ttgcgggaag catgcattga actgagcgat tttgtccgtg gcaatgaaaa
     9421 agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt
     9481 gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg
     9541 tacggacatt aatccactgt atcgtctcaa caaacgtcag ggtaaacagt tactggcagc
     9601 attaggttgc ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg
     9661 cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct
     9721 ggaagggaaa aacgtacctc aacaggtcgc cagaacaata gagaactggt atctgaaaac
     9781 cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt
     9841 tgcacctatt ggattgtgca aaaaagagta atttgttcac gccggatgcg gcgtgaacgc
     9901 cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa taggcctgac
     9961 aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctt
    10021 cccggctgtt acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa
    10081 cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt
    10141 cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca
    10201 ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac
    10261 gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgcaccg
    10321 caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt
    10381 ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg
    10441 tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt
    10501 ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt
    10561 gttatggtct gttgggactg gaaccgttaa gtcgcggtcg tgcgtgtttt cgttcagcgc
    10621 taaaacgttg cgccggagca tgctacggta aagagagcca tgaggaacat gcgctacgct
    10681 tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggg gcggtggcgc
    10741 tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg
    10801 gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc
    10861 acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac
    10921 ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg
    10981 ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag
    11041 cagtttgtcg cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc
    11101 aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgcgt
    11161 cataatattg aaatccatcg acatctgccc tgccgtcagt ttcgctttta ccacctggtc
    11221 cgccgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagtggt ctgcgctttc
    11281 aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccataccag gaaacaaaga
    11341 aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc
    11401 cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca aattcaccga
    11461 cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc
    11521 gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg
    11581 ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac
    11641 tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag
    11701 ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca aaattagcat
    11761 taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt
    11821 gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct tcagcttttg
    11881 ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcgacgttct tccagcggcg
    11941 gacgtttcat ctggtcacgc tggcctttca tgatttcgcg aatctgctgt ttctgcgcgt
    12001 cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg cctttgtggt
    12061 gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca tgggccaggt
    12121 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
    12181 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga
    12241 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
    12301 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
    12361 atattacaac accatgattt tgcagagatt atgaagaact ataccggatg actggtgata
    12421 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaggtaac ttaatacccg
    12481 cagaaagatt tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa
    12541 tcccagcgct accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa
    12601 acgttcctca ccgtcctgcg ccagcaacgt tcctttctta aacggcataa aattcagcgt
    12661 gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg
    12721 ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcgat
    12781 tgcgctggca gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc
    12841 aagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg taaacgtacc
    12901 accaggctcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcaggaa
    12961 tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc
    13021 acgaattgcg gtatgtagat caaggtgcca gcgcacagat tctttgccct ggtcataaaa
    13081 atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tccccgcttt cagcaaacag
    13141 ctgccaacga ccgccaaaca ttcggttcat atcgctatgg caataacgtt tcccttgctt
    13201 cagcgcagga ggattcccga ggatcaccag caaccgccac cgtaacggga tctcgccgtg
    13261 agatatcgcg ccaagcaatg catcaagcat ctccacaggt gccgtctcat taccgtggat
    13321 ccccgcagaa atcaccagag cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc
    13381 atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgatga caggtttttt
    13441 actcgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc tggaacggat
    13501 aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc ccttcgcgca
    13561 gcaattgcgg gtcggccaga tcggcagcag taagacgatc gcggtagtaa cgatccaccc
    13621 agtcattgag cgcattaaac aaggtatcgt tcatcatcac cgccggattc accgcccggc
    13681 gttcttcttg tgtcaatacc acccgcaacc gcaggcacgc cgggccaccg ccattcgcca
    13741 tgctttcacg taaatcaaag acttttagtt cgctaatcgg gttgtcagcg gcaaggagtt
    13801 cattgagata accccatact ccggcgtgtt cccgacactc ctgaggcagc accaacatca
    13861 tggaaccatc atcgcggctc aacagttggc tgttaaacag atatgtagac accgcatccg
    13921 acacggaaac ctgagttgcc ggaacttcta tcgccataaa accgttcacc cgcgcacgca
    13981 ggtttgccag taactgtgac tggcgagcga acgcctgttg gtggcaaaac agcacctggc
    14041 ggttactcac ggcaatcacg tcattatgaa aaacgccctg gtcgataacg tccgggtttt
    14101 gctgggcgaa aatcacctgt tggggattca cctgattcag ccttgccacc gcctcgctgg
    14161 cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt atcattgccc tcttctcgcc
    14221 cgtagacaaa aagttgcata cccggttcac cgtaatgacc gccgagacga ttgtggtttg
    14281 ccgccccctc atcaccgagc aaggctacct gcggcaacgc cgaatggaca ctaaatttct
    14341 cttcgtcgtt aaaaatcgct tttaacagcg attcagtgac gggcgcttcc agcgaacggt
    14401 gaaatttatt gttcaggtta gcaacggtga gatgcacttt gccatccagc gtatcggcag
    14461 atggcgcgat cgttgccgca ttggctaccc acattggcga agcggaactg acgctggaaa
    14521 gccagtgcgg tgcctggcgt acaacctttt ccagtacctg ctcatcgcta ccactgaatc
    14581 ccaactgacg cagcaccgga ataaacggac gctcgtgagg cgggatcacg gcctggggga
    14641 atcccgcatc ggcaagggtt ttcattttca gtaagccctg ttttgccgcc agtcgcgggt
    14701 tagagacctg aaaacggtga cgggtagagg ctttattacc aaacgacagg cccgcgtaat
    14761 gatgcgtcag ccctaccagc ccgtcgaaat taacttccca ggcgttcatc gcaccacctc
    14821 atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa gttaacgagt ccgactccag
    14881 gctcgccata ggccatgcgc agtaatctgc ggcataccag gcgctggggc gatggttacc
    14941 ggatgcacca atgccgccga atggcgcggt actggcagca ccagtaagcg gtttgttcca
    15001 gttaacaatc cccgcccgcg cctccagcaa cagttgatcg aatttttccc gctcggggga
    15061 aaccagaccg caagagagtc cgaagcgagt gttattcgcc attcgaatcg cttcctcgaa
    15121 agaatcataa cgccagacgc gcaataacgg tccgaacacc tcttcatctg gtacgccagc
    15181 aacgcctgtc atttcaatga tccccggcgt cagcaacgat gtccctgctt gtaataagcg
    15241 cggcgcaagc agggttcgtc cacccatcgc ttccagttgc tgccaggcag taaacacctg
    15301 ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc tgcggttcgt catcccagtt
    15361 gcccggcgtt aatcgctggc tgacggcaac cagacgagca agaaatgcat cgccctgcgc
    15421 cccgcttttc agcaatacac ggcgggcgca ggtgcagcgt tgcccggctg taacaaacgc
    15481 cgactgaatg gtcagatgga cagccgcgtc gatatccgcc acctcatcga taatcagcgg
    15541 gttattgccg cccatctcaa gggcgagaat tttctccggc tgaccggaga gctggcgatg
    15601 cagctggtag ccggtattgg cgctgccggt aaacagcaag ccgtcgagat cctccagcgc
    15661 actcagcgcc tgacccgttt cacacccgcc ctgcaccagg ttcagcacgc ctggcggcaa
    15721 gccagcctgc tgccataaac gcattaccgc ttcgccactc cacggtgtca gttcgctggg
    15781 tttaaagata atggtgttac ctgccagcaa tgcgggaacg atatgtccgt tcggcaaatg
    15841 accagggaaa ttatacggcc caaacaccgc cagcacgccg tgcgggcgat gtcgcaggct
    15901 cgccgcaccg tccggcattt cactacgctg ctcgccggta cgaacgtgat acgccttaat
    15961 tgatatcgcg attttattga tcatcgccgt caattcggtt gccgcctccc agcgcggctt
    16021 gccggtttct cgggcaatga ttgcggttaa ttcgccttta ttcctttcca gcaatccggc
    16081 aaagcgttcg acaacggcct gacgttcagc aaatgagagc cgcgcccagc gcggaaacgc
    16141 cgcacgggct gcccgacaag cctgcccgac ctgagcggca tcggcatcat tgccttgcca
    16201 taacacctcg cccgataccg gattacgctt cacacgcgat gcaccctggc ccgttatcca
    16261 gtcaccgtta atccataaag tcatgctgtt ttctcctctg cgcacaggcg caccagacga
    16321 acgcgatccc ctgcatggca tttgagggca tccagttgtg cggcggttaa aatcaaacgc
    16381 tcggttgccg gatcggtacg caccagcacc acgcggaaat ggtgataatt ttcattggcg
    16441 accaggcagg ccggaaaatc gccctgcgca ggctgccctt ctgccacttc caccagccga
    16501 cttttacgga tggcgcgcac gcggtcgatg tcacactcaa gcgtcggccc accgtcgaag
    16561 atgtcgatat agttgcggta gcgaaaacct tctttctcca gcaccgcgcg ggcaggcgcg
    16621 gtttgcggat gtacctgacc gatgacgtcc tgggcttcct gggataaaaa gtgcgtatag
    16681 atcggatgtt tcggcatcag ttctgcaata aatgcctttt gcccggtgcc gcagagaaaa
    16741 tcggcgcggc taaaatccat cgaaaagaag cgtttaccga ggctttgcca gaacggtgaa
    16801 tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca ctttgtcatt aaacttgtcg
    16861 cgaaaagccg ccataaacat aaagcgcgat ttcgacagca aatagccgtt gccctctttg
    16921 cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc tgccggtgtg atcgttactg
    16981 agaaacaacg tcggcaatgc gttatagaca ttcagctctt ttgaggcgtg aaccaacgtg
    17041 ccgacgcgat agttgtacca gggatcgttc agcccaactg ccacctcaat ggcgcaaatc
    17101 cccgccacgg tgcctgtctc gctatcttcc agtacaaaca cataaccctg ctcacttttg
    17161 ggcagttcac cttgccaggt tttgattgcc ctttcgatac gcgccgaaag cgtggcttca
    17221 ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg caagctgcat cagcgccgag
    17281 acatctgagc gctcaacggg acggatgacc atcatgatga acctcggcta acaaagtgtt
    17341 cgcaagcaac tgcaaagcga tccagtccgg tcgtcacctc ttcttcgctg acattgagcg
    17401 caggcgcaaa acgcaccacg ttgccacccg caatcagtac catcacgcct gctttcgccg
    17461 cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc attcagtaca cagccaatca
    17521 gcaaacctaa gccgcgaact tcattgaaca aaccatagcg gtgattaata atattaagac
    17581 gctcaacaaa ccagtcgtga cgttgcttaa ccccgttaag catctctggc gtattgatga
    17641 gctccagcac tttgcctgcc accgccgagg ccagcggatt gccgccatag gtggtgccat
    17701 gagtgccaac ggtcatcacg ctggcgcact cttcggttgc taacaacgca ccgaccggga
    17761 aaccgccgcc cagagctttg gcggtagtta acagatcagg cgtcacgccg tagtgcatat
    17821 aggcatacag ttccccggta cgcccgacgc cggtttgtac ttcatcaaaa atcaacagcg
    17881 cattgtggcg atcacacaat tcacgcagac cttgtaaaaa tgcgttgctg gctggcacca
    17941 caccgccttc accctggatg ggttcgacaa tcaccgcaca ggtagagtcg tcaatcagcg
    18001 cgctggcaga gttaatatcg ttatatgcag catgacggat atctggtggc agtggcgcaa
    18061 aatcctgtga ataggctggc tgcccacccg cactaacagt aaacagcgtg cgaccatgaa
    18121 acgcattttt aaacgccacg ataccgctct tatggctgcc gtagcggtcg tgagcgaatt
    18181 tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc ggagttacaa aagaaaacgc
    18241 gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg cagtaccgac tcgttggtgt
    18301 aaccgttgcc ggtatgccag aacttactcg cctgttcgtt cagcgcctca cgcagttccg
    18361 gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc gaagtcgata tactctttcc
    18421 cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg tataaacggt gccggagcgt
    18481 aaacaggtat catccattca tcaaagtttt cacgcgtaat tggctgagac atagcgacct
    18541 ctacagtaaa tagctcgtta tttatatgtt aataataagt aatgttttcg ctgtaaatgt
    18601 agattgcagg gttcgtgcca gccagtgata aaagtgcata aacggcggag gctaactgga
    18661 aatcaaggag ttataaccaa accatatgca tttaaagtgc atattaagtg aatacgtttg
    18721 cgatgtgggt gaataaaaag aataaaaaac gcaatgttat gcagaagtaa aatataattc
    18781 tggaattgtg atcattgacg aaatttactg gaaattactg cgccattctg acgcagcgcg
    18841 caccaaaagc gggcattttt tgcgccatcg ttgacatcat taacaaccat cgatcaaatc
    18901 acttaacaac aggcggtaag caaagcgaaa ttctgctacc atccacgcac tctttatctg
    18961 aataaatggc agcgactatg aaatttgtct cttttaatat caatggcctg cgcgccagac
    19021 cccaccagct tgaagccatc gtcgaaaagc accaaccgga tgtgattggc ctgcaggaga
    19081 caaaagttca tgacgatatg tttcccctcg aagaggtggc gaagctcggc tacaacgtgt
    19141 tttatcacgg gcagaaaggc cattatggcg tggcgctgct gaccaaagag acgccgattg
    19201 ccgtgcgtcg cggctttccc ggtgacgaag aagaggcgca gcggcggatt attatggcgg
    19261 aaatcccctc gccgctgggc aatgtcaccg tgatcaacgg ttacttcccg cagggtgaaa
    19321 gccgcgacca tccgataaaa ttcccggcaa aggcgcagtt ttatcagaat ctgcaaaact
    19381 acctggaaac cgaactcaag cgtgaaaatc cggtgctgat tatgggcgat atgaatatca
    19441 gtcctggcga cctggatatt ggcattggcg aagagaaccg taagcgctgg ctgcgtaccg
    19501 gaaaatgttc tttccttccg gaggaacgcg aatggatgga gagactgatg agctggggat
    19561 tggttgatac cttccgccat gcgaatccgc aaacagcaga tcgtttctca tggtttgatt
    19621 accgctcaaa aggttttgac gataaccgtg gtctgcgcat cgacctgctg ctcgccagcc
    19681 aaccgctggc agaatgttgc gtagaaaccg gcatcgacta tgaaatccgc agcatggaaa
    19741 aaccgtccga tcacgccccc gtctgggcga ccttccgccg ctaatttagc agctctcctg
    19801 gctcaaactg ggtcaggaga attaaccttg agaaaaatca acaaactgtc agtaatgatt
    19861 tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt agttgtctga ccacttctct
    19921 attatcaagt ttgatatagg aaactccacg atgaacgctg agcgtaaatt tctttttgcc
    19981 tgtcttattt ttgcgctggc catttacgct atccacgctt tcggtttatt cgatctgctc
    20041 accgatttac cccacttaca gacactcatt cgccagagcg gattgttcgg ttatagcctc
    20101 tatattctgt tattcatcat tgccaccctc tttctgttac caggaagcat attggtgatc
    20161 gcgggaggaa tagtttttgg cccgttctta gggacactac tctcattaat tgccgccacg
    20221 ctggcctcct cgtgctcatt cctgctggcg cgctggatgg gacgtgattt actgctgaaa
    20281 tacgttggcc atagccatac cttccaggcc attgaaaaag gcattgcgcg taacggtatt
    20341 gattttctta ttctgacccg cttaatcccg ttgtttcctt acaatattca aaattacgct
    20401 tacggattaa ccacaatcgc cttctggcct tataccctta tttcggcact cacgaccctg
    20461 cccggtattg ttatttatac cgtgatggca agcgatctcg ccaatgaagg cattacgctg
    20521 cgctttattt tacaactctg tctggcgggc ctggcgctgt ttattctcgt ccagctcgca
    20581 aaactctacg cccgacacaa acatgtggat ctgtctgctt cgcgccgcaa cccacttact
    20641 cacccaaaaa atgaaggata gaacgatgtt gcaacattat tcagtgtcat ggaaaaaagg
    20701 actgactgca ctctgcttac tggctgttgc tgggcttagc ggctgcgatc aaaaagagaa
    20761 tgcggcggca aaagtggaat acgacggact ttcgaacagc caaccgctgc gcgtcgatgc
    20821 caataaccat acggtaacca tgctggtgca aattaatggt cgtttcctca ccgacgacac
    20881 tcgtcacggt attgtgttta aagatggctc caacggacat aaatcgctgt ttatgggtta
    20941 tgcgaccccg aaagcatttt atgaagccct gaaagaggca ggtggtacac cgggcgaaaa
    21001 catgacgatg gataataaag aaacgactca tgtcacaggc agcaaactgg atatttcggt
    21061 caactggcaa ggggcggcaa aaacgtattc cttcgatgaa gtgattgttg acagtaatgg
    21121 caagaaactg gacatgcgct ttggcggtaa tttaacggca gcagaagaga agaaaacggg
    21181 ttgcctggtg tgtctggata gctgcccggt cggcatcgtc agcaatgcaa catacactta
    21241 tggtgcggtt gaaaaacgag gtgaagttaa attcaaaggc aatgcctcgg ttctcccggc
    21301 gaataacacg ctggcaacgg ttacctttaa aatcaccgaa taaagccagg ataaaggatg
    21361 atgaagatgc aatcgcgtaa aatctggtac taccgtataa ccctcatcat cctgttgttc
    21421 gctatgctgc tggcatgggc gctgcttccc ggcgtccatg agtttatcaa tcgcagcgtt
    21481 gcggcgtttg ccgccgtgga ccaacagggt atagaacgct ttattcagtc ttacggcgca
    21541 ctggcggcag ttgtctcgtt cttgttgatg attttacagg ccattgctgc accgctacct
    21601 gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg cgttctgggg cggctcgctg
    21661 tcgtggacca gttcgatggc cggcgcggca ctgtgctttt ttatcgccag agtgatgggc
    21721 cgcgaagtgg tggaaaaatt aaccggcaaa accgtacttg acagtatgga cggctttttc
    21781 actcgctacg gcaaacacac catcctggtc tgtcggttat tgccttttgt ccctttcgat
    21841 ccaatcagct atgctgccgg tttgacttca atacgttttc gctcgttttt tatcgccacc
    21901 gggcttggtc agttaccggc gactattgtt tattcctggg cgggcagcat gttaacaggc
    21961 ggtaccttct ggtttgtcac cggactgttt attctgtttg ccctgaccgt ggtgattttt
    22021 atggcgaaga aaatatggct tgagcgccag aagaggaatg cctgatgggt ttaccgccgc
    22081 ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct gcatcgtcgc cattacggcg
    22141 aggtgctaag ccccattcgc tggtgggggc ggatcccgtt tatcttttat ctggtgtcga
    22201 tgtttgttgg ctggctggag cgcaaacgct caccgctcga tccggtagta cgatcgcttg
    22261 tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg tgtggatatc accagtatga
    22321 aagtcgccga gcgcaccggc agcagcgata aactgctggc agtggctgac tggcggcaaa
    22381 gcccgctctt tagcgatgaa gaacggctgg cgctggagta cgccgaagcc gcaagcgtaa
    22441 cgccgccaac ggtcgatgat gccctgcgta cccgactggc tgcgcatttt gacgctcagg
    22501 agctcaccga actgacggca ttgatcggcc tgcaaaatct gtcagcccgt tttaattctg
    22561 ccatggacat tcccgctcag gggctgtgcc gtattccgga aaaacgttct taaggagaga
    22621 tgatgcgcca ttgtgggtgg ttgctgggat tgttatcgct gttttctctg gcaacacatg
    22681 ccagtgactg gcaagaaatt aaaaatgagg ccaaagggca aactgtctgg tttaacgcct
    22741 ggggcggcga taccgcaatt aaccgctatc tcgactgggt tagcggcgag atgaaaaccc
    22801 attacgctat aaacctgaag attgttcgtc tggcggatgc cgcagacgcg gtgaagcgca
    22861 ttcagaccga agctgctgcc ggacgtaaaa cgggcggctc ggtggatctg ctctgggtga
    22921 acggcgaaaa cttccgcacc ttaaaagagg ccaatttact gcaaaccgac tgggcagaga
    22981 ctctgcccaa ctggcgctat gtcgacacac agctgccggt gcgggaagat ttttcagtgc
    23041 ctacagaagg ggctgaatcg ccctgggggg gcgcacaact gacatttatc gcccgccgcg
    23101 atgttacgcc acagccacca caaacgccgc aagccttact ggagtttgct aaagccaatc
    23161 ccggcacggt tacctatccg cgcccaccgg actttaccgg cacggcgttt cttgaacagt
    23221 tgctgattat gctgacgccc gatcccgccg cattaaatga agcgccgaac gatgcgactt
    23281 tcgcccgtgt cactgctccc ttgtggcaat atcttgatgc gctgcatccg tatttgtggc
    23341 gcgaaggaaa ggatttcccg ccttcgcccg cgcggatgga tgctctgctg aaagccggaa
    23401 catttcgcct gtcgctgacc tttaaccccg cgcatgcgca gcaaaaaatc gccagcggcg
    23461 atttgcctgc aagcagttac agttttggct ttcgcgaggg gatgattggc aacgtgcatt
    23521 tcgtcaccat tcctgccaac gcgaatgcca gtgctgcggc gaaggtagtt gccaatttct
    23581 tgctctcacc cgatgcgcaa ttgcgtaaag cagatcccgc tgtctggggc gatccttctg
    23641 ttctcgatcc gcaaaaactg cctgatgggc agcgcgaaac attgcaatca agaatgccgc
    23701 aagatctgcc gccggtactg gctgaaccgc acgcaggttg ggtaaatgcg ctggaacaag
    23761 aatggctaca ccgttacggt acgcattaat ctttttgctg tgggcgatgg tggcggtgat
    23821 ttatgcaccg ctgatcccgg cagctctcac gctgatatcg cctgctttgt cgttgacaca
    23881 ctggcaggcg ttatttgccg atccacagtt accgcaggca ttactggcaa cgctggtgtc
    23941 gacaaccatc gcggcggtcg gggcgttgtt gattgcccta ctggtgattg tggcgctgtg
    24001 gcctgggccg aaatggcagc gtatgtgcac ccgtctgccg tggctgctcg ccattcccca
    24061 tgtggctttt gccaccagcg cccttctgct ctttgctgac ggagggctgc tttatgacta
    24121 tttcccgtat ttcactccgc caatggacaa attgggtatt gggctgggct tcacgctggc
    24181 ggtgaaagaa agcgcatttc tgctgtggat cttagcagcg gtattgagtg aaaaacggct
    24241 tttacagcaa gtcatcgtgc tggattcgct gggctacagc cgctggcaat gcctgaactg
    24301 gctgctgttg ccctccgtcg cgcctgcgct ggcaatggcg atgctggcga ttgttgcctg
    24361 gtcgctgtcg gtcgtagatg tggcgattat tcttgggcca ggtaatccac cgacgctggc
    24421 agtaattagc tggcagtggt taactcaggg cgacgccgat caacaaacga aaggcgcact
    24481 cgccagcctg ctgctgatgt tgttactcgc cgcctacgtt ttgctgggct atctgctatg
    24541 gtgtacctgg cggcgtacta ttccccgcgt agatggcgtt cgtaagcctg ccacgccttt
    24601 attgccaggc attacgctgg cgagtttttt acccttgacc agtgtgctgt gtgtggttct
    24661 gctggcgatc ctcgcggatc agtcgacgat caatagtgaa gcgctcatca acagcctgac
    24721 aatggggctg gtggcgacat tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg
    24781 gccacagcgt cgccagttgt ggctatggtt gccgatttta ttaccggcac tgccgctggt
    24841 ggcgggccag tacacgctgg cgctatggct gaacatggat ggaagctgga cggcggtggt
    24901 ctgggggcat ctgctgtggg taatgccgtg gatgctgttt atcctgcaac ccgcctggca
    24961 gcgcattgat tcacggttaa ttttgattgc acaaacactg ggctggtcgc gggccaaaat
    25021 cttcttttac gtgaaatgtc cgcttatgct acgccctacg ctgattgcct tcgcggtggg
    25081 attttcagtc agtattgcgc agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc
    25141 gacgctcacc actgaggcgg tggcattaag cagcggcggc agcaacggta ttctcgccgc
    25201 cctggcttta tggcaactgc tattaccgct tattattttt gccctgaccg cgttagtcgc
    25261 aaaatgggta ggttatgtca gacaaggact ccgctaatgc tctgcgtgaa aaatgtttcg
    25321 ctacgtttac cagaaagttg cttgctgaca aacgttaact ttacggtgga taaaggtgac
    25381 attgtcacgt taatggggcc gtctggctgt ggaaaatcca ctctgttttc atggatgatt
    25441 ggtgcactgg ccagacagtt ttcttgtaca ggtgagctat ggctcaatga gcaacggatt
    25501 gacatgctac ccaccgcaca gcgtcagatt ggcattcttt ttcaggatgc actgttattt
    25561 gaccagttca gtgtcgggca aaatttactg ctggcgctac cggcgacact taaggggaat
    25621 gcccgacgta atgccgtgaa tgatgcactt gagcgttcag gccttgacgg agctttccat
    25681 caggatcctg ctactttgtc tggcggtcag cgagcgcgcg ttgctctgct tcgcgccctt
    25741 ctcgctcagc caaaagcgtt gctactggat gagccattca gccgtcttga tgtggctctg
    25801 cgcgataatt ttcgccagtg ggtgttcagc gaagttcgcg aactggcgat ccccgtcgtt
    25861 caggtaacgc acgatctcca ggatgttcct gctgatagtt ctgttctgga tatggcgcag
    25921 tggtcagaaa attacaacaa actgcgataa cgcaaagttt ttctcaatgc gtcagttcag
    25981 aatggcgcac tcaaaactac aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc
    26041 gcgctggcaa tggctttagg gctggcttgc gcttcttcgt gggccgctga actggcgaag
    26101 cctcttacac ttgaccagct tcaacaacaa aatggcaaag cgatagatac tcgctccagc
    26161 gcgttttata acggctggcc acaaacctta aatggccctt ctggtcatga acctgccgcc
    26221 ttaaacctct ctgctagctg gcttgacaaa atgagcaccg aacagctcaa cgcgtggatc
    26281 aagcaacata acctgaaagc cgatgccccg gtggcgctgt acggtaatgg caaagatgtc
    26341 gacgccgtca aaacgcgact gcaaaaagca ggttttacgc atatctccat tctgagtgac
    26401 gcgctaagcg aaccttcccg tctacaaaaa ctgccgcact ttgagcagct ggtttatccg
    26461 caatggctgc acgacctgca acaaggtaaa gaggttacgg cgaaacctgc cggtgactgg
    26521 aaagtcattg aagcggccag gggcgctcct aagctttacc ttatcagcca tattcccggt
    26581 gctgactaca tcgataccaa cgaagtggaa agcgaaccgc tgtggaacaa agtttctgat
    26641 gaacaactaa aagcgatgct ggcaaaacac ggcattcgcc atgacaccac ggtcattctg
    26701 tacgggcgtg acgtatacgc tgcagcgcgt gtggcgcaga ttatgcttta tgccggcgtg
    26761 aaagatgtgc gcctgctgga tggcggctgg caaacctggt ccgacgcggg tctgcccgtt
    26821 gagcgcggta cgccaccgaa agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca
    26881 caaccgcagt tgatgctcga tatggaacag gcccgtggac tgctgcatcg ccaggatgca
    26941 tcgctggtga gcattcgttc gtggccagaa tttatcggta cgaccagcgg ttacagctat
    27001 attaaaccaa aaggtgaaat agccggagca cgttggggac acgctggtag cgactcgacg
    27061 catatggaag atttccataa tccggatggc accatgcgta gcgccgatga tattaccgct
    27121 atgtggaaag catggaatat caaaccagat cagcaagttt cattctactg cggcaccggc
    27181 tggcgcgcgt ccgaaacctt tatgtacgca cgagcaatgg gctggaataa cgtctccgtt
    27241 tacgacggcg gctggtacga atggagcagc gatccaaaaa atccggtagc aaccggtgaa
    27301 cgcggcccgg acagcagcaa ataacattgc aattactgac gctggagcga cttcagcgtc
    27361 agatacccac tccacacccg cgtaaaggtc gtcatccagc acaacgcacc gaagatccac
    27421 gcaaaccacg caaaccatgc agggaataaa catcccagca caaacagtaa gatcgtttct
    27481 gtgccttcgg ttaatccccc cagataataa aacgatttat gcgcataacc gggattatca
    27541 atctgatatt tcgccgccag cgcagcaaaa gcgagaaaac tgctacccgt gccgataaac
    27601 gcaaacagca gccagccgcc tgccagggcg ttttgctgcg gtgcggcgag gataaaccca
    27661 aaaggtacca gcgcgtaaaa aagaaaatcg agagaaatat caagaaagcc gcccgcgtcg
    27721 gtaagccctc tgcgccgcgc cagcgcaccg tccagaccat caagcagcct gttcaacaaa
    27781 atgacgacaa gcgccgccag ataccagccc agcgccagaa aaggcagtgc cagcacaccg
    27841 atggcaaatc ccactaatgt taaaccatct ggcgtaatgc ccggtttatc aagaacccgc
    27901 acacactgat gcaacaacgg tttaatccgg ggatgaagat ggcggtctag cacaggcact
    27961 ccttaaatat aaagcctttc tgattgagca acagtgcgga tattatggca tttttcgctt
    28021 atctgcccgt gtgtaattta tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga
    28081 tggcaaaatt ttactcgcgc aacgccccgc ccagagcgat caggcgggat tatgggagtt
    28141 tgccggtggt aaagtcgagc cggatgaaag ccagcagcag gcgctggtgc gtgagttaaa
    28201 cgaagaattg ggcatcgaag caactgtggg tgagtatgtt gccagccatc agcgagaagt
    28261 ttcgggccgg attatccatc ttcatgcctg gcacgtaccc gacttccacg ggacgttaca
    28321 ggcacatgaa catcaggcgc tggtctggtg ctcacctgaa gaggcgctgc aatatccgct
    28381 ggctcctgct gacattccat tattagaggc gtttatggct ttacgcgccg ccagaccagc
    28441 ggattagtgc taagggtttt gtcatcccgc tggcattgca gcagtattcc ttcggcttta
    28501 attaccgccc cttcagaata atttttatcc tgataaacgc agcactgagt acagggctgc
    28561 gctgactgcc cgcctgaact gaccacttct ggcggtacgt ttacctccac gtccggacca
    28621 taatgcgggt tagccagtgc aattaatgga aatgctaata ctacggcgaa caatgctcga
    28681 ctcacaggga actccttaac gttattgtct ctgctactga taacggtagc cgggtggcaa
    28741 aactttagcg tctgagttat cgcatttggt tatgagatta ctctcgttat taatttgctt
    28801 tcctgggtca tttttttctt gcttaccgtc acattcttga tggtatagtc gaaaactgca
    28861 aaagcacatg acataaacaa cataagcaca atcgtattaa tatataaggg ttttatatct
    28921 atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat
    28981 caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa
    29041 caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg
    29101 atccagtttc gcgtggtatg ggttgatgat cgcaatcagg tacaggtcaa ccgtgcatgg
    29161 cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca
    29221 gttaaccttt ccattcttaa attcctcggc ttcgaacaaa ccttcaaaaa tgccctgact
    29281 actctgccga tgggcggtgg taaaggcggc agcgatttcg acccgaaagg gaaaagcgaa
    29341 ggcgaagtga tgcgtttctg ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg
    29401 gataccgacg ttccggcagg tgatatcggg gttggtggtc gtgaagtcgg ctttatggcg
    29461 gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca
    29521 tttggcggca gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa
    29581 gcaatgctaa aacgccacgg tatgggtttt gaaggcatgc gcgtttccgt ttctggctcc
    29641 ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact
    29701 gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca
    29761 cgtcttatcg aaatcaaatc cagccgcgat ggtcgagtgg cagattacgc caaagaattt
    29821 ggtctggtct atctcgaagg ccaacagccg tggtctgtac cggttgatat cgccctgcct
    29881 tgcgctaccc agaacgaact ggatgttgac gccgcgcatc agcttatcgc taatggcgtt
    29941 aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag
    30001 caggcaggcg tactatttgc accgggtaaa gcggctaatg ctggtggcgt cgctacatcg
    30061 ggcctggaaa tggcacaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca
    30121 cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt
    30181 gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg
    30241 atgctggcgc agggtgtgat ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc
    30301 tacaaatggg cacaattcat tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc
    30361 cccggcaaaa tttcaggcgt ttatgagtat ttaacggatg atgctcccca gggaacattt
    30421 cttatgggcc aacggcattt cttactgtag tgctcccaaa actgcttgtc gtaacgataa
    30481 caagcttcaa gttcagcatc cgttaacttt ctgcgatagc agcagatatg ccagtaaaga
    30541 aatcccatta gactattttt ttgataatct tcttcgcttt cgaacaactc gtgcgccttt
    30601 cgagaagcaa gcattatata atgccaggcc agttcttctt caattgtccc gttttgaaaa
    30661 gctgtgcttg atatcgagat catccatgat aattccgccg cccatattag cttcgccgag
    30721 gatttaccgg agctatgatt agcgcaatca gagatatagt ctgagggaaa aacagcaaat
    30781 ttattcaaca aggcgataac ctgctctggg gcttcctcca tgtttgcttt aaaggtattg
    30841 gctccatggt cgccagaaag aaaatgctcc attaaggcac aataactttc gctatcttcg
    30901 ataccccatt gttcctctaa agactcgcgt cttttactta tgatatcgat cgagtcaaaa
    30961 ggaagcacat gatattggaa ggtatctttg ccaggttcag gctttcgcgg ccagaactcc
    31021 agcgtttcag accattgctt atgatagaat cgataaggtg cgatcaattg tagcgcctgt
    31081 aatttctcga tactgagcgg ctcaatacct ttagcctgat aataatgcag ctgttctttt
    31141 tttgctttaa aaccggcccg aacaataagc cccatcataa ttaatagata aagaaaagag
    31201 catcccgcgg taatcaggcc tctttcattc aaaccgttgg atgttatcgc tgcgaataca
    31261 aacattacag cgacaacaca tgttaaataa aacccccact tacaaagcag catggtctta
    31321 ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac
    31381 ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca
    31441 tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc
    31501 gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt acgctatcgc
    31561 cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
    31621 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
    31681 cggagtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
    31741 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
    31801 catgtccggt cgcgtagcca tttctggcag cgaatggaat aacgcttttc cggcgtcggt
    31861 ggagtggata tagcgccctt ttttgatgag gaaaccacgc ttgaacaaca gttcaataat
    31921 cccggcacgc gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
    31981 tttgtcctgc acaaagcgcg cgatcccggt catcgccgaa agcagtgttg catcggtaaa
    32041 atggcgcggc ggctgggttt gccgctctac cacttcacct ttttcacaca gcaactcatc
    32101 gcctttcgcc accacaggca atggcgtacc gtcgttttct tcatcgcgct ctttgctgcc
    32161 taacagcgtg cgccagcctg cttcagcaag aaaacgcgct ttagcgacaa atttgccttt
    32221 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggcaga actgcatcag
    32281 atactgacgg gaaatcaggt tatagatctt cgcttcgttc tccgtcaggt tgatcgcaga
    32341 actccgtgcg gtcggaatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
    32401 gttgcgtata tctggatcta ccactggctg cggcaacaga tctggtgcat gaacgctgat
    32461 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcgga
    32521 acgcggataa gtgattagct tgtgcgtttc gtacagcttc tggcagatat caagcacgtt
    32581 ctgcgcgctc agaccaaagc gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg
    32641 cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgacaatag ccggttgacc
    32701 actaatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
    32761 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
    32821 tgtcacgata tgtgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
    32881 atcgcggcgc accaccagcc caagcacggg cgtctgcacg cgtcccacgg aaagcacgcc
    32941 ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatattga tgccgtacag
    33001 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacagc ggtacaaact cactgttgga
    33061 acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
    33121 ctgcacctgc tggcgctttt ccggtgccag ttgtagatag tccagcactt catccaccag
    33181 cagttgccct tcgcgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaggta
    33241 ccgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt gccacttttc
    33301 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
    33361 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
    33421 gataaagcca tcgcctttcc ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
    33481 actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc atggcacgac
    33541 cgccgcgtgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
    33601 tagctttgac ctcattttcg gcttccggca tgaccgccag cagcaaaccg ccagaagttt
    33661 gcggatcgca cagcaaatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
    33721 tggcaaagtt acgttcggtg ccgccaggca ctgcacccaa cttaatgtac tcttcaaccc
    33781 ccggcaattt cgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
    33841 tttcgctcaa atggcccagc aggccaaagc ccgttacgtc ggtcatcgct tttacgcctt
    33901 caatgttggc aaaggacgcg cctgcgatgt tcatccggca catcacttcc gtcgccagtc
    33961 cctgatgttc tggtttcagc agtgattttt tctcagccgt ggtaagaacg ccgatcccca
    34021 gcggtttcgt caggaacagt ttacatccgg cttgtgcggt actgtttttc ttcacccgct
    34081 cggtcggtac gatccccgtt accgccagac caaaaatcgg ctccggcgca tcgatggagt
    34141 gaccgccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct tcggttactt
    34201 cgcgggcaat ttccggggaa agtttgttaa tcggccagcc gaggatcgca attgccataa
    34261 tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
    34321 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
    34381 cattgcccag atcgtacacc gccgcatcgt cgcgggtttc attacccaca agcaaattcg
    34441 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
    34501 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
    34561 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
    34621 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
    34681 ataagtgaca aacggcgtcg ggtccgggac gtttatcgac gtagatgctt tcagctgcgg
    34741 cgtgccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
    34801 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaacc cctgggcaac
    34861 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt cccatcgcgg
    34921 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
    34981 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
    35041 cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat gccacggttg
    35101 catggactta tggtccggcg cgcgcatacc cgcacgcagg atgttttgca gttgttcacc
    35161 cgttggtgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
    35221 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
    35281 tgttacagca cagtccgcaa ttcctgctga caagtgccgg ttgggtcatt acgataacca
    35341 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
    35401 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
    35461 ggtacttaac ctgttcttta tttttctcgt gctggttggt gtggggatct ggatgcaggt
    35521 cagtggtggt gattcgaaag aaacggccag tcgtggtgca ctgctgctgg acatttctgg
    35581 tgtgatcgtc gataaacccg acagttctca gcggtttagt aaattaagcc gccaactact
    35641 tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca ataccattcg
    35701 ccaggcgaag gatgaccgca atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg
    35761 cggcgaccaa ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
    35821 gaaaccggtt tatgccgttg gcgagaacta cagccagggg caatattatc tcgccagttt
    35881 cgccaataaa atttggctgt caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa
    35941 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
    36001 gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gacatgtcac cggcagcccg
    36061 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
    36121 taaccggcag atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgagggttt
    36181 aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg tcgatgcact
    36241 ggcatcgagt gcggaaatcg aaaaagcact gaccaaagag ttcggctgga gtaaggctga
    36301 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
    36361 tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg aaactcaggg
    36421 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
    36481 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
    36541 tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat cgatgggcgg
    36601 catggcggca tctggtggtt actggatttc cacgccagct aattacattg tggctaaccc
    36661 cagcaccctg accggttcta ttggtatctt cggcgtgatc accaccgtag aaaatagtct
    36721 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcgg atgtttctat
    36781 caccagggca ctgccgccgg aagcgcagca gatgatgcag ttaagcattg agaatggcta
    36841 taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc agattgataa
    36901 aatcgcccag ggccacgtct ggaccggtca ggacgcaaaa gctaacgggc tggtcgatag
    36961 tctcggggat ttcgatgatg cggtcgccaa agcagcagag ctggcaaaag cgaaacagtg
    37021 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaacatgtc
    37081 tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg caccgctggc
    37141 ctcggtagcc tctaccgtta aaagtgaaag cgacaagctg gcggcgttta acgacccaca
    37201 aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taaatcctgc aaaaatgtgg
    37261 ccgatatgct gtcggccaca ctcctgaatc ggactgtctt tcactccctc tctccactac
    37321 attcccttgc tcatttagca tctgatatgg agtcatcagt gaacaaggcc ccctctttaa
    37381 ttgccgctat cgtcctcgga ctggggatta gcgcctgtgg ttattttgtt ggcgatggtg
    37441 ttaaacattt aaaaaccaac aaccgttatg tcaatgtgcg cgggctttct gaaaaagaag
    37501 ttcgcgcaga tacggcggaa ttaacaattg cgattaactt taagggtaat gttcccggcg
    37561 aactgttccc gaaactggaa gaagcgcaga aaaaaattgt tgccgaactc aatgctcagg
    37621 ggattaacga gaaagagata attctgggcc aatggacaag caagcgaaca gattcctttt
    37681 atctgaaaga tgatcccaca atgccacgct ataacgcaga cgggtcagtg acgataaaaa
    37741 cccataatgt tgctgctgtc gaaaaagtcg tcgcgaaatt aaacgaactg caagtcgcta
    37801 cggatggcgc tatcgctgag agcaaagtcg cctatcgctt taatggtatt ggcgcactgc
    37861 gcgctgagat gattgctgcc gccaccaaag atgcacgcaa cgccgcacta caatttgcaa
    37921 cggactcagg tagccaggtg gggtcaatta gcgatgcctc acaaggcgta ttccagatct
    37981 ttgccagtgg cagtgatgaa gatgatccta cggcgattaa taaaacggta cgcgtagtca
    38041 cgaccgtaac ctatgcgttg caggattaac cacttttctc cggcgggaca ctaatccctc
    38101 cgtcctgccg gacttttctc atatccacag agtatccagg tgcgctcgct gcccctcacg
    38161 tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta
    38221 cgttgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt atataccggt
    38281 gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc
    38341 agatttcacc attcatgaat atacgccgct gatggattct tcagatatga cgccggaaga
    38401 ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat
    38461 tctgcacggc accgacacga tggcgtatac cgcctctgcg ctgtcgttca tgctcgaaaa
    38521 tctcggtaaa ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga
    38581 cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt
    38641 aacactcttt tttaataacc gactgtatcg cggcaaccgc actaccaaag cccatgccga
    38701 tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat
    38761 tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg gaattgatcg tacatccaat
    38821 caccccacaa ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg
    38881 caattttctg cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc
    38941 gccacaaaac aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt
    39001 ggtcaacctg acacaatgta tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa
    39061 cgccctcgcc catgctggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac
    39121 caaactgcat tacctgctta gccaggaact ggatactgaa accattcgca aggccatgag
    39181 ccaaaacctg cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg
    39241 ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg
    39301 aaggtgacag tacggtggat gtcgctaacc gcctgattga ctggtgccag tcgcgcggtg
    39361 aagcggttat cgccagtcag gactggcacc cggcgaatca cggcagtttt gccagccagc
    39421 acggtgtaga gccttatacg ccaggccaac tcgacggttt gccacaaacc ttctggccag
    39481 atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga
    39541 tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg
    39601 ataacggccg tcggcagaaa acctctctcg atgactggtt acgcgatcat gaaatcgatg
    39661 aattgatcgt tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt
    39721 tacagttagg ttataaggta aacgtgatta ccgatggttg tcgtggcgtg aatatccagc
    39781 cccaggacag tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc
    39841 tggcagactg ggaagagacg caggggtaat tttacgctgg cctacaattc tgtactggca
    39901 ttgtaggcca aataaaacac gtcagtggca catctggcaa ttgatgccat caacgaaaga
    39961 tttaatttac ctcagaaatc tcttccaacg acaccttccg cgtttcgatg ccaaaaatgg
    40021 agagaaccag cgcacagagc aataacatga caccaagtac cataaacacc gtgatcgacc
    40081 cataatgtgt taataatgcc gcaacgccat agggcgtgaa gactgcgacg atccgtccga
    40141 cggcattaac gaaacccgaa ccgcgcaggc gtaaatgcgt tggccaaagc tccgggatat
    40201 aaaccgccga cgcgaagcaa acatacatgt ataaaaagaa gatcatcacc aggccataga
    40261 ttaaaatcgc ccactctgta gtctgaattg aatagatata gcctaacact gcaataataa
    40321 taagtaaggt ggagccaaat aaccgacgag gaaaatgatc aataattaat gccgcaataa
    40381 atattcctac cggagcgcca atcataataa cagcggtcat taatattgat ttatcgacat
    40441 caatgccgga gttaacaaat atggtcggta tccatacggt gatggtataa agcgaaatgt
    40501 tcattgcaat taaaacagta atcgcgacta atgtacgtcg taacatttca cctttaaaca
    40561 ggagccagaa agtacccttg attactttaa ccttgctgtt gctctgatac gaagtaagcg
    40621 ggggtaaacg aatactcttc tctctttcaa tttgctgctc tacttcacga agttggcatt
    40681 ctgcacctgc gatttgccct ttccctgcca gccatcgtgg cgactcaata aagtatttac
    40741 ctgagagaaa ccaggctaac agtatgccaa taccacccaa cagaaacatt atccgccaac
    40801 taaaaaaagc gataaccacc acacctatcg ccgcagacag catgggcgac cagttaccaa
    40861 caaatgagag ccgcgcggac cattttccac gcaccgtcgc ggggataaac tccgtaaatg
    40921 aggcatagcc aaccataatc agcgccccca ttcctgttcc catcaggaag cgaaagaaga
    40981 taagccagta catatcaggg acaaaagcgg cccctgttgc agcaataccg acgatgagaa
    41041 gatttatgcg aaacgccctg cgccgcccaa agtaatcacc gatgaaccca ccggtaagag
    41101 agccgatgaa ataaccaaac attaatgccg aggtaaaggc ggcattgagg aaattatttg
    41161 accatccatt gcttaccagc tttgctaaga cgacattacc ggagtaactc aaaaaccct
//
LOCUS       DAZGXS010000026        40175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGXS010000026 DAZGXS010000000
VERSION     DAZGXS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKG5_001528"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKG5_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0867653.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKG5_001529"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKG5_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0867654.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHKG5_001530"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHKG5_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0867655.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHKG5_001531"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHKG5_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0867656.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHKG5_001532"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHKG5_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0867657.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHKG5_001533"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHKG5_001533"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0867658.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHKG5_001534"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHKG5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0867659.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHKG5_001535"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHKG5_001535"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0867660.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHKG5_001536"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHKG5_001536"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0867661.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHKG5_001537"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHKG5_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0867662.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHKG5_001538"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHKG5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0867663.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHKG5_001539"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHKG5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0867664.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHKG5_001540"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHKG5_001540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0867665.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHKG5_001541"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHKG5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0867666.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHKG5_001542"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHKG5_001542"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0867667.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHKG5_001543"
     CDS             complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHKG5_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0867668.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHKG5_001544"
     CDS             complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHKG5_001544"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0867669.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15607..16794
                     /gene="metC"
                     /locus_tag="ACHKG5_001545"
     CDS             15607..16794
                     /gene="metC"
                     /locus_tag="ACHKG5_001545"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0867670.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16934..17593
                     /gene="yghB"
                     /locus_tag="ACHKG5_001546"
     CDS             16934..17593
                     /gene="yghB"
                     /locus_tag="ACHKG5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0867671.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHKG5_001547"
     CDS             complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHKG5_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0867672.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHKG5_001548"
     CDS             18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHKG5_001548"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0867673.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHKG5_001549"
     CDS             19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHKG5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP0867674.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHKG5_001550"
                     /pseudo
     CDS             21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHKG5_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHKG5_001551"
     CDS             21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHKG5_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0867675.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHKG5_001552"
     CDS             complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHKG5_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0867676.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHKG5_001553"
     CDS             complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHKG5_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0867677.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHKG5_001554"
                     /gene_synonym="parF"
     CDS             complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHKG5_001554"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0867678.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHKG5_001555"
     CDS             complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHKG5_001555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0867679.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHKG5_001556"
     CDS             complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHKG5_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0867680.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHKG5_001557"
     CDS             complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHKG5_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0867681.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHKG5_001558"
     CDS             complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHKG5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0867682.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32271..32930
                     /gene="qseB"
                     /locus_tag="ACHKG5_001559"
     CDS             32271..32930
                     /gene="qseB"
                     /locus_tag="ACHKG5_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0867683.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32927..34276
                     /gene="qseC"
                     /locus_tag="ACHKG5_001560"
     CDS             32927..34276
                     /gene="qseC"
                     /locus_tag="ACHKG5_001560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0867684.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHKG5_001561"
     CDS             34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHKG5_001561"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0867685.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHKG5_001562"
     CDS             34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHKG5_001562"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0867686.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35357..36244)
                     /locus_tag="ACHKG5_001563"
     CDS             complement(35357..36244)
                     /locus_tag="ACHKG5_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0867687.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36241..37188)
                     /locus_tag="ACHKG5_001564"
     CDS             complement(36241..37188)
                     /locus_tag="ACHKG5_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0867688.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37199..38236)
                     /locus_tag="ACHKG5_001565"
     CDS             complement(37199..38236)
                     /locus_tag="ACHKG5_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0867689.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38245..39228)
                     /locus_tag="ACHKG5_001566"
     CDS             complement(38245..39228)
                     /locus_tag="ACHKG5_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0867690.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39225..40034)
                     /locus_tag="ACHKG5_001567"
     CDS             complement(39225..40034)
                     /locus_tag="ACHKG5_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0867691.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        10027 a        10792 c        10073 g         9283 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttacca ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgtaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa
     2221 catccattac aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tcgcgctggg cgttactggc gcagagtacg atgcctggct gatacgcata
     2341 ggcgatggtg atcaattctc cagcggcttt gtcgaagtga acccaaattc gaagatcccg
     2401 gcgctgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa acggactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc
     2761 ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt ataaacatgt gcaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataagc gtcaggggta aggattggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagaccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag cagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aacctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gctggataat ttcaacggcg
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3841 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagagc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
     4261 gcgttaagcc gatgtgtggc tccagcgatt ccggaattac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttgctga tgataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     4981 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5041 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
     5101 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5401 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5461 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccgcgcgtca gccattccgg gtagaacgca gcgataaccg cagtatcgac
     5581 cttgtaaacc tgctcaacaa agtcactcag cttgtcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatt gggttagcga ccccacctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcaggtgcca
     5941 ggtcgaaaca cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagc gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaataggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgcaagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggtcaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccctgtg ccgctggtcc acacttgaat gatgttattg gtatacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8641 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg taatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcactgcg
    10501 caggccagtt ccgggtaagc gaccgctgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcatttcccg gataacctcc aacggaattt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 actaccacca cggctggcgt tttgccgctc atttttgcgg gtttaaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaaaaat ctccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc tggctacgat atgtggcccg
    11581 gcccttacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactca ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    11941 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctacg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attggttggt gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12241 tggcgcagat cgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccattaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatcgag ttgtgagacg
    12601 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    12901 gcaacagcaa atcaacatat ccgttaaatc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagccct ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt
    13381 tcgctggttc acgaagcgca taaggcctta ggtgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcaa gtattatcac cacttcgtca atccaggcat accagccaag cccgcattta
    13621 ctggactatg cagctacgaa ggcggcgatt ctgaactaca gccgaggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagca aacgccgatg
    13801 aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13861 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    13981 gaaaacgcct tatccgccta caaatgcatg aagattcaac atgttgcaat agattttgta
    14041 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14101 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14161 tgaacgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14221 gacctatctt caggtaaccc gcctgatgca gcgtgtccat taccttcatc aacgtctcgt
    14281 aatcgacagt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14341 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14401 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14461 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14521 tcagcaggac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14581 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    14641 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14701 gcaacaatac ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgcg
    14761 cgaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14821 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    14881 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagcccaacg aacggcgaaa
    14941 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15001 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15061 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15121 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15181 gctcgcgctt aaggcgacgc ttctgattaa agaactctac gctcttactg aagaagattg
    15241 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15301 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15361 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15421 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15481 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15541 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15601 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15661 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15721 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15781 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    15841 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    15901 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15961 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    16021 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    16081 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16141 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16201 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16261 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16321 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16381 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    16441 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    16501 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16561 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16621 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgcagccatt
    16681 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16741 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16801 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16861 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    16921 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16981 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17041 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt
    17101 ggctggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17161 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17221 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    17281 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17341 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17401 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17461 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17521 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17581 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17641 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17701 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    17761 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17821 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    17881 tagcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17941 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18001 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18061 tacgcgcatc cagtggtcgc gccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18181 cttcgccaat atccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18301 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18361 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18421 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18481 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18541 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18601 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18661 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18721 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18781 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18841 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18901 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18961 aactggttcg cgaacagaaa gtgactttcc tgctgggggt tggcggcggt tctgtactgg
    19021 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19081 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19141 tgccagcaac cggttcagaa tccaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19201 ataagcaggc gttccattca gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19261 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19321 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19381 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19441 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19501 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19561 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19621 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19681 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19741 tgcctaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19801 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19861 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19921 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    19981 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20041 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20101 agcgttagaa gtgggttatc gctcgtttga taccgccgcg gcctataaga atgaagaagg
    20161 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20221 gctgtggaac gacgaccaca atcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20281 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20341 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20401 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20461 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20521 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20581 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20641 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20701 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20761 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20821 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    20881 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20941 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21001 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21061 ttaccgtgca cgcacagccc ccctcacaga ccgtagaaca aacagtccgg catatttatc
    21121 agaactataa atcagatgcc actgcccctt attttggtga aaccggagag cgtgcgataa
    21181 cttctgtgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggtaata
    21241 ttggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgtagcgata accgaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ccgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggtcaatg ccttctataa aaccaccttc aagacggcca acaatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 cattcatatt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 acggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaatagcaa
    21961 ttagccggaa caataaaaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    22021 tgttctggca acctgcgtgc tcagtggctg cgtcaatgag caaaaggtca atcagctggc
    22081 gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    22141 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    22201 acaggactat tttgattgcc tacgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    22261 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    22321 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    22381 gcgtctgaca ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt
    22441 tctggcgacg agcctcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    22501 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt aacggccagt
    22561 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    22621 gtacgaagac gtcttcactc ttataaccaa tcttcgccag cgggtttttc ccggtgtaat
    22681 acatggtggt cgagttagcc agtggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    22741 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    22801 gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    22861 cgaacagctc tttaaagcgg tcataactgc ccatgcccgg cttcatcatc ttcgacaacg
    22921 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    22981 gttctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcaatca
    23041 gaatcttttt aatgcctttc agatcgcgag cgcggcgata gaggttgatc gtcggttcgt
    23101 ggttagtgtc catatgcgga caaatatccg ggtaaacgca ggacaaacga cggcaggttt
    23161 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    23221 cggaaattac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    23281 tcgaatcttc ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag
    23341 agcagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    23401 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    23461 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    23521 cgtgtttttg catcaatgcg cgggcgcaac ctgggttagt ttcgtggtgc agaatacgcg
    23581 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt
    23641 aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct
    23701 ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga
    23761 ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca
    23821 gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc
    23881 ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca
    23941 tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat
    24001 gcgcggtacg gcgcagacta gcctcaatac caccgaggat caccggtaca tctttccacg
    24061 cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt
    24121 tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca
    24181 tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcatga
    24241 aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag ccctgtgctt
    24301 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    24361 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    24421 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    24481 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    24541 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc
    24601 ctcctcgggc gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc
    24661 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    24721 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    24781 tctgtcttcc gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc
    24841 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    24901 cgcggtgaca tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc
    24961 cagactgata tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg
    25021 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag
    25081 ggtagaaacc agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc
    25141 cgcttcgcca caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg
    25201 gcgctcgccc ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg
    25261 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    25321 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    25381 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    25441 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    25501 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    25561 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    25621 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    25681 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    25741 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    25801 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    25861 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagcag
    25921 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    25981 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    26041 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gtttcccgca aaaatacaga
    26101 ttgccgactt aaacttttcc ggcggcttcg cgctctgcga cttctttatc gagctcggcg
    26161 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    26221 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    26281 ttaagattaa tcttattcga agttgtggag acgcacacgg gaataatcgg gacgcccgcc
    26341 gcaattgccg cgtgaaatgc cccagtcttg aacggtagca ggccgcgacc acggctgcgg
    26401 gtaccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cactacttcc
    26461 gcaatagtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    26521 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    26581 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    26641 tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    26701 aacagcggcg caagacgacc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    26761 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    26821 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    26881 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    26941 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    27001 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
    27061 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    27121 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    27181 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    27241 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    27301 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    27361 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    27421 aagcatcttc aattaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    27481 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    27541 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    27601 ccccaggcgg caacgttaat ttgccggtga gcggttcgcc ctgaccacgc gccgacggca
    27661 gcgtaatcgg atcaatggca tagctacgac cggtggaatc aacaaacact accggttggt
    27721 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    27781 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    27841 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    27901 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    27961 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    28021 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    28081 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    28141 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
    28201 gcaaaccttc gaggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    28261 ggcgcacggt atcgcggcgg aacaccagcc attcggagag aatttccagc aggtttttca
    28321 ccgccggacg accatccaga ccgatcatat taaggttaat gcgatagctc ttttccagat
    28381 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    28441 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    28501 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    28561 gatgcggcaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    28621 gcactgaacc acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa gtgataattt
    28681 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
    28741 tcggctggtc gattaatgcg attgccgcct gagccacttc acgcaggtta tgcggtggaa
    28801 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    28861 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    28921 tcccctgccc caactcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    28981 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    29041 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    29101 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    29161 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    29221 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    29281 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    29341 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    29401 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    29461 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
    29521 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
    29581 gtagatagga atcagcggtg cctgctggtt gacgatcact tctgcctgct gatacaacgc
    29641 attacgcttt gtcgcatcag tgatctgcat ggcctggttt agtaaggcgt catactgcgc
    29701 atttttccag tgaccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
    29761 agcatcattg tacgtcgcat cccacgactg ccgagacagc atgaaatcac cggctcgtcg
    29821 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
    29881 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
    29941 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca aggcttttgc
    30001 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
    30061 ttttacctct ggcggcgtca gcgtagttgc gggcgttctc aaccccagta ccttttgcgc
    30121 aataagctgt cgatcaaccg taagatatag cgcccgacgc actcgcacat cgttaaatgg
    30181 cggtttctca aggttgaagt tgtaatattc gctgttcaga cgcggaataa ttcgtatctc
    30241 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
    30301 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
    30361 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
    30421 ttcgttaact acccactgat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
    30481 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
    30541 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
    30601 atccgtcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
    30661 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
    30721 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt cggaccactg
    30781 caaaccgcta cgcagatgga aaatatagcg cctgccgccg tccagtatct cccagcgttc
    30841 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
    30901 cagcacaatc tgcgcggcag tattctcctc caccttttgc gggtcgagcg tacctgggtc
    30961 gctatgattg ttgtaacgaa agacttgttg cggggcgagc tgtgtgttgg tgggaacgtc
    31021 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
    31081 catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt ggcattgttc
    31141 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
    31201 cggcacataa acattcgttt ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta
    31261 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
    31321 aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcgag ccacggcata
    31381 acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa tcggttcgct
    31441 aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca
    31501 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
    31561 gataaatttc gccacgctgt agttgagcaa ctcaggccta cagcggtgcg gcaacatcgc
    31621 cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg tcatgttgcc
    31681 tccctgcgaa attattcccg ggaacgcatc ccgggagcgg taacaattac ggatttactt
    31741 tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact tcaccctgaa
    31801 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttgtgg tcgatatcaa
    31861 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
    31921 tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
    31981 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
    32041 aaaaaccgcc ctgctctgct gccatcaccg gtgcgctgca cagggccatt actgcgatta
    32101 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
    32161 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttagtgctgt acagagcgcg
    32221 ttacaacacg gtttactagc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
    32281 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
    32341 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt tatagcgcac
    32401 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
    32461 gcgaatggcg agaaaaaggt cagcgtgagc cggtactgat cctgaccgcg cgcgatgcgc
    32521 tggaggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
    32581 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
    32641 gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
    32701 ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
    32761 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
    32821 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
    32881 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
    32941 acgtcttagt ctgcgtgtca ggctgacgct aatcttttta attctggcct cggtgacctg
    33001 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg aattgttcga
    33061 cacccaactg atgctgtttg ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc
    33121 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
    33181 gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
    33241 agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac tggtgggcga
    33301 taaagatcaa tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    33361 tggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg ggcaattgat
    33421 cccgtggttg gtcgcactgc cggttatgtt aatcatcatg atggtactac tgggtcgtga
    33481 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg actcggaaaa
    33541 accactaaac gcgactggcg tacccagcga agtgcgacca ctggttgagt cgctaaatca
    33601 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    33661 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg cgcagctctc
    33721 tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg ggatcgatcg
    33781 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    33841 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    33901 ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgtcc agggcatcaa
    33961 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    34021 gcgttacagt ccacagggca gcgtggtgga cgtcacgctg aatgccgata atttcatcgt
    34081 aagggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    34141 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    34201 cgccaagctg catggcatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    34261 gaaggtaagc tggtaagggt aagacttttg ctaaattcgc aaaagacttt gcacattttg
    34321 ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt
    34381 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    34441 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    34501 ttcgcatcgt tcgcgccgac agcgactacg atgtcaaagc ggaagtacaa aactttctct
    34561 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    34621 aaaaatacat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    34681 gtacccgcaa agatccgtcg aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat
    34741 atatgctttc tctgacctgg aacgcgccaa tggaagcctt caccgaaaaa gatcagttct
    34801 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    34861 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    34921 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    34981 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    35041 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    35101 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    35161 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcat cttgaagcgc
    35221 atctgcaaac cccgcacatg aaggcgtata gcgaagccgt aaaaggtgac gtgctggaga
    35281 tgaatattcg tattctgcag ccagggattt aacattcctg aaaagaggcc ggtgttgcta
    35341 ccggcctcag gtcagattag ccattctttc ttcccgtttt cagcccccga tggaaatcgt
    35401 taattcgctt aatggtttta accgcacgct gcggcaccgc ggaagccacc gaccagacaa
    35461 tcatttccag gcacaacaat accgtaccat gtagtggcgt tttccccttt tcatccccgc
    35521 gcggaacatg aatcacgata ctggcgtctt tgctaaaacg tgaatccagc gcgtttgtca
    35581 gcaaaataac agggatcccc agccgtctcg cttcacgcag cgttgtcagc ccttcccggt
    35641 gcgcagattt ttgcgccatc atgatcagaa catcgccacg ttgaagcgca atcagttgct
    35701 cggcaaggcc aatacccgta cggtttagtg ccgtggcagg caaacctatg cggttgaata
    35761 gtcgtgcggt atagtccgcc agaatacccg aagcgccaat gccaaaaatt gcgacttgcc
    35821 gtgcctgtac caacaacgct accgcttgcg caagcgcgtg gcggttttca ggctctgaca
    35881 ggacattaca ggtatacaaa tgcccttcaa gaacaaagtc gattgcggtg ttaacatcgc
    35941 tcgtcaagtt actcaccgtc gtagacatct tttcgctgga actaagggct ggaccaagcc
    36001 actgttccag agtgcgtttc agatcacgca gcccggcaaa ccccagggcc tgtatagccc
    36061 tgataactgt ggcatcggaa gtgttcaacg tggccgcaat ttccatcgcc gtttgatcga
    36121 gtaccacttc acgatgctca ttaatatagc tggcgacagc tcttaaccca ggcgttaact
    36181 gactcgcgcg agtgcgaaaa cgctcaccat aaatatcaac ccgcgttttg tctttctgaa
    36241 tcatttggcc tccggtaaag gtcgccccgc aatttgtgat tgtatctgcg cagccatcag
    36301 ccccagagat gcaaaggagt tagaaattgc ctcttcacga gaaatcagca cataacgccc
    36361 ggaacgacag gcagtcagaa actgacacca tcccggcatg actttttcca ttgctgccag
    36421 ctcatcctga ggtttaccac cggtgtcacc gcgccaggtt gcaaaaacaa aatcggcatc
    36481 cagttcaggt aaacgctccg cgctcacatc cattcgccca ccttcaggaa tgctctcaat
    36541 cagcggcgga aagcgaaaac cagcatcgcg taatacccgc cccagcgaat gatagctatg
    36601 catcacatta atttttccct gatttgcctg aatcaccgat accgttattt tttggctgtc
    36661 cagcgtcgcc ttcagcgcat tgatttgtgc ctgatagcgg cgttcaagta tcgccagctg
    36721 cgactgcgtt cctgtcagtt ctgccagctt gcgatagatt tctggcgcgc cccctttgag
    36781 atgatcaatg ctcaccgtgg gcgcaatttt ttccagtcgt tcaatgggtg tattacgggt
    36841 cggttctgtg atgatcaggt caggctttgc cgccacaatc gcttcaatat caatatcagc
    36901 ggtgccaata aaagcgatcg atgaattatc aaagtcgaca cctgtcaata acgcgcctga
    36961 tcgaatgaaa tggctaccgt caggccgagt tcgcccgtgg ctggcgacag gaggtacacc
    37021 gagctcaatc agaggaatag tgatatcaag atcgtgtaaa gagacaattc tctttggatg
    37081 agcaggaaca gtgactttgc gccctaaatc atcagtaaag acctgaacag gttctgtcgc
    37141 gcgggcaaag aatgacaaca gtatcagcag agaaaaaaat aaacgcatat aactcccatt
    37201 aaaaactgtt cctgcgctgc cagagcagta gcaaaaagaa tggtccacca atcaatgaaa
    37261 taatgattcc ggcaggcagt tgcaaaggca aaaaagccaa acgaccaatg ctatccgcca
    37321 ggatgactaa caacgccccc gatacggcgc tccccagcag taatgtcgtc tgcccgccac
    37381 gtagaataaa acgtgatata tggggtgcta tcaatccgac aaaaccgatg ttaccaacac
    37441 acgaaacgca gacggctgtc aggattattg gtgcggcgac acgtaacaac gccagacgag
    37501 aactattcac ccctaacccg gttgcaacct gatgaccaag cagagcgata tccgccgtcc
    37561 tggcggtgaa cagcaataaa agagcggctg gcaacatcca acaggctgaa ataccgacta
    37621 acatccagtt agcagcatgc aggcttcccg ataaccacat caatgcagtt tgtacatctc
    37681 gaacatctgc agtggtcata aatacaccga taccggcggc gaaaaaccag gaaacaccaa
    37741 taccaatcag aacaaatcgc gggcgggaaa tttcgcgtgc acaaaaaatc acgattaaag
    37801 ccaccaacaa gccaccagca agaccagcca ctggacgcca gaacattccc agcattggaa
    37861 actggaaaat cagccacagc accgccacgc tacatccttc tttgacgccg attaaaccag
    37921 gatcggcaag gccgttacgc gcaatggatt gcatcgcggc ccccgccata cctaacatgg
    37981 ctccgcatag caatgccatg atgactctcg gcaagcgaat atcctgcaca atataacgtg
    38041 cgtctgtgct tacgttttct ggagcaaaca acgctcgtcc tacttccgat gccgggacaa
    38101 aaaaagaacc atgcatcaga ccgaatatca gtattccaac cgcaaacaga aacagcccac
    38161 caagcaacac cagattttta gggcgtacca gtgtcgaaaa cttaccaaca cgtagtggac
    38221 gtagcccgac gttcattatc cgactcattt gaacattctc gtcgccataa tgacaaaaac
    38281 aggcgcacca actaacgccg tcatgacacc ggtcgctaac tcatggggcg tgaaaagcgt
    38341 ccgagcaata atatctgcca gtaataacaa aagcgcgccg acacaagctg aaagagggag
    38401 cagaacgcgc aaatccgctg ataccagacg cctgacaatc tgcggtacta gcagaccgac
    38461 aaaaccaatg gggccagcaa tcgatacggc tgcgccacag agcaacgcga tcgcaagcag
    38521 agtgaatgtt cgcgtgcgga gtaccgagac gcccagcccc tgcgccatgc gatcacccag
    38581 tgccaacatg ttaagagagg gcgcaaggta aatggctaac acaacccccc ccagagaaaa
    38641 ccaggcggac gtacccagcg tcgcccaatc ctgccccgct aaatcacctg ctagccatgt
    38701 ccgcatcgcc agcagtgttt gttcgtcaag aatcaggatt gcagccgtta ttgaggacac
    38761 gaacgccgat agcgccacgc cgcataaagt aactttcatt ggcgttagcc ctgaacgccc
    38821 tgcggaggaa agcagcaata tcaacaggaa caataacgcg ccgcccgtag atgccagcag
    38881 tgggcgtcca accggaaagg ccagcccaag cgcactggca gcaacgacag caagagcggc
    38941 tccggcattt aagcctaaaa tatgtggctc ccccaatggg ttacgaatga cagcctgcaa
    39001 caaagcacca gcaactccaa gagaagcacc ggtaagcaat gctgccgcta aacgcggcaa
    39061 acggagtctt acaattacgt tgtgatcgaa gttttgcggt tcaaaatgaa gaaatgcctg
    39121 caacaccgtt tgtggtgcaa taaatcgggc accaatccct ggatatacca cgcatcccac
    39181 aagcagtaag gcgaggaaaa ttacagtaac cagccctgtg cgcatcatac tttttctaca
    39241 ccacgaaacg gcatgaagaa cggctttccc gtcagagtat ttattgatgc gtgaacatca
    39301 acgtcaaaga cagcttttac cagctcaggc gtgcaatgct ccccctcatt caacacacgc
    39361 accactttcc cctggcgtaa aaatatcaac gtatcaccat aattgacggc aaaattcaga
    39421 tcatgcaaca ccacgacgac tgtacgcccg tgatgacgcg ttaaatcatg taataactca
    39481 agaatttcaa cctgataacg taaatcaagc caggttgtgg gttcatccag taggatataa
    39541 ggcgtctttt gcgccaacac catcgcaatc cagcaacgct ggcgctgccc accagagagt
    39601 ttttcaacag gtaaatgagc aaactcctgc gtaccggtta atttcagggc ctcttctacc
    39661 gcctgctcgt cggcgtcgct ccattgacgt atgaaatttt gccagggaaa acgcccacgc
    39721 gacaccagtt cataaacggt taagccctcc ggcaataatg gcgactgcgg caagatccca
    39781 agtctacggg ataacgcttt cgttggttgc tcgtgaatcg ctttaccatc cagcaatatt
    39841 gagccgccca tgggttgcaa gacacgtgca atggtgctga gcaacgtcga ctttccacag
    39901 ccgtttgccc caaccagaac ggtcattttt tctgtgggaa cggagaaaga aacaccatca
    39961 acaatgattt tcttgtgata cccggcagaa acattgtcga ggatcagccc ctgccctttt
    40021 gcgttatgtg ccacgtgtac gccaacttaa tcaaagaaaa acaaaaataa aactcattct
    40081 cttttgagag tttgacgcca caaaatgtag tagtcaagaa gttaaaagcc atacaaaaac
    40141 aaggtaaata acaggtgaaa tatttttaaa gataa
//
LOCUS       DAZGXS010000027        38513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGXS010000027 DAZGXS010000000
VERSION     DAZGXS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            91..1029
                     /gene="ytfI"
                     /locus_tag="ACHKG5_001568"
     CDS             91..1029
                     /gene="ytfI"
                     /locus_tag="ACHKG5_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFP0867692.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(1092..1646)
                     /gene="ytfJ"
                     /locus_tag="ACHKG5_001569"
     CDS             complement(1092..1646)
                     /gene="ytfJ"
                     /locus_tag="ACHKG5_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0867693.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1971..2177
                     /gene="ytfK"
                     /locus_tag="ACHKG5_001570"
     CDS             1971..2177
                     /gene="ytfK"
                     /locus_tag="ACHKG5_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0867694.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2273..3616)
                     /gene="paeA"
                     /locus_tag="ACHKG5_001571"
     CDS             complement(2273..3616)
                     /gene="paeA"
                     /locus_tag="ACHKG5_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0867695.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3939..4577)
                     /gene="msrA"
                     /locus_tag="ACHKG5_001572"
     CDS             complement(3939..4577)
                     /gene="msrA"
                     /locus_tag="ACHKG5_001572"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0867696.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4783..6516
                     /gene="tamA"
                     /locus_tag="ACHKG5_001573"
     CDS             4783..6516
                     /gene="tamA"
                     /locus_tag="ACHKG5_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0867697.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6513..10292
                     /gene="tamB"
                     /locus_tag="ACHKG5_001574"
     CDS             6513..10292
                     /gene="tamB"
                     /locus_tag="ACHKG5_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0867698.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10295..10636
                     /gene="ytfP"
                     /locus_tag="ACHKG5_001575"
     CDS             10295..10636
                     /gene="ytfP"
                     /locus_tag="ACHKG5_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0867699.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10848..11099
                     /gene="chpS"
                     /locus_tag="ACHKG5_001576"
     CDS             10848..11099
                     /gene="chpS"
                     /locus_tag="ACHKG5_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP0867700.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            11093..11443
                     /gene="chpB"
                     /locus_tag="ACHKG5_001577"
     CDS             11093..11443
                     /gene="chpB"
                     /locus_tag="ACHKG5_001577"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP0867701.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11523..12053)
                     /gene="ppa"
                     /locus_tag="ACHKG5_001578"
     CDS             complement(11523..12053)
                     /gene="ppa"
                     /locus_tag="ACHKG5_001578"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0867702.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12363..13319
                     /gene="ytfQ"
                     /locus_tag="ACHKG5_001579"
     CDS             12363..13319
                     /gene="ytfQ"
                     /locus_tag="ACHKG5_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0867703.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13459..14961
                     /gene="ytfR"
                     /locus_tag="ACHKG5_001580"
     CDS             13459..14961
                     /gene="ytfR"
                     /locus_tag="ACHKG5_001580"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0867704.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14975..15997
                     /gene="ytfT"
                     /locus_tag="ACHKG5_001581"
     CDS             14975..15997
                     /gene="ytfT"
                     /locus_tag="ACHKG5_001581"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0867705.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15984..16979
                     /gene="yjfF"
                     /locus_tag="ACHKG5_001582"
     CDS             15984..16979
                     /gene="yjfF"
                     /locus_tag="ACHKG5_001582"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0867706.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(17012..18010)
                     /gene="fbp"
                     /locus_tag="ACHKG5_001583"
     CDS             complement(17012..18010)
                     /gene="fbp"
                     /locus_tag="ACHKG5_001583"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0867707.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            18186..19559
                     /gene="mpl"
                     /locus_tag="ACHKG5_001584"
     CDS             18186..19559
                     /gene="mpl"
                     /locus_tag="ACHKG5_001584"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0867708.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19715..20266)
                     /gene="yjgA"
                     /locus_tag="ACHKG5_001585"
     CDS             complement(19715..20266)
                     /gene="yjgA"
                     /locus_tag="ACHKG5_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0867709.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20360..21712
                     /gene="pmbA"
                     /locus_tag="ACHKG5_001586"
     CDS             20360..21712
                     /gene="pmbA"
                     /locus_tag="ACHKG5_001586"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0867710.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21896..22282
                     /gene="cybC"
                     /locus_tag="ACHKG5_001587"
                     /pseudo
     CDS             21896..22282
                     /gene="cybC"
                     /locus_tag="ACHKG5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(22327..22791)
                     /gene="nrdG"
                     /locus_tag="ACHKG5_001588"
     CDS             complement(22327..22791)
                     /gene="nrdG"
                     /locus_tag="ACHKG5_001588"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0867711.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22981..25119)
                     /gene="nrdD"
                     /locus_tag="ACHKG5_001589"
     CDS             complement(22981..25119)
                     /gene="nrdD"
                     /locus_tag="ACHKG5_001589"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0867712.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25513..27168)
                     /gene="treC"
                     /locus_tag="ACHKG5_001590"
     CDS             complement(25513..27168)
                     /gene="treC"
                     /locus_tag="ACHKG5_001590"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0867713.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27218..28639)
                     /gene="treB"
                     /locus_tag="ACHKG5_001591"
     CDS             complement(27218..28639)
                     /gene="treB"
                     /locus_tag="ACHKG5_001591"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0867714.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPVLLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28758..29705)
                     /gene="treR"
                     /locus_tag="ACHKG5_001592"
     CDS             complement(28758..29705)
                     /gene="treR"
                     /locus_tag="ACHKG5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0867715.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29890..29943
                     /gene="mgtL"
                     /locus_tag="ACHKG5_001593"
     CDS             29890..29943
                     /gene="mgtL"
                     /locus_tag="ACHKG5_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0867716.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            30084..32780
                     /gene="mgtA"
                     /locus_tag="ACHKG5_001594"
     CDS             30084..32780
                     /gene="mgtA"
                     /locus_tag="ACHKG5_001594"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0867717.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32986..33372)
                     /gene="ridA"
                     /locus_tag="ACHKG5_001595"
     CDS             complement(32986..33372)
                     /gene="ridA"
                     /locus_tag="ACHKG5_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0867718.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33445..33906)
                     /gene="pyrI"
                     /locus_tag="ACHKG5_001596"
     CDS             complement(33445..33906)
                     /gene="pyrI"
                     /locus_tag="ACHKG5_001596"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0867719.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33919..34854)
                     /gene="pyrB"
                     /locus_tag="ACHKG5_001597"
     CDS             complement(33919..34854)
                     /gene="pyrB"
                     /locus_tag="ACHKG5_001597"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0867720.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34858..34992)
                     /gene="pyrL"
                     /locus_tag="ACHKG5_001598"
     CDS             complement(34858..34992)
                     /gene="pyrL"
                     /locus_tag="ACHKG5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0867721.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35273..35668)
                     /gene="yjgH"
                     /locus_tag="ACHKG5_001599"
     CDS             complement(35273..35668)
                     /gene="yjgH"
                     /locus_tag="ACHKG5_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0867722.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35799..36512)
                     /gene="bdcA"
                     /locus_tag="ACHKG5_001600"
     CDS             complement(35799..36512)
                     /gene="bdcA"
                     /locus_tag="ACHKG5_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0867723.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     SRDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36583..37176
                     /gene="bdcR"
                     /locus_tag="ACHKG5_001601"
     CDS             36583..37176
                     /gene="bdcR"
                     /locus_tag="ACHKG5_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0867724.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37321..37773
                     /gene="tabA"
                     /locus_tag="ACHKG5_001602"
     CDS             37321..37773
                     /gene="tabA"
                     /locus_tag="ACHKG5_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0867725.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37896..>38513
                     /gene="yjgL"
                     /locus_tag="ACHKG5_001603"
     CDS             37896..>38513
                     /gene="yjgL"
                     /locus_tag="ACHKG5_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFP0867726.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARLLNNHQKLNNLQKLNNLQKLNN"
BASE COUNT         9394 a         9479 c        10271 g         9369 t
ORIGIN      
        1 attttaataa tgagcgtttt ttgatagttt acttctatag tgagatattt aatggcgata
       61 taaagtaacc aaataaaata aggttgtcat atgttaccca ggatcagaca caataatttt
      121 attggtgcgg tggagttatt tgtaaagtct tcgtatacaa aaacacattc aaacaatttt
      181 tttaacaaca tccatcatgc atttaagaaa aaagattgga tttcgaatta tgatagcctg
      241 ttaactttga gggagttctt tcgttgcgca actcagattg ataaaagtag ttatcaggtg
      301 ctttcatcaa aaaatgaaac tgttaacgct atggataagt ttcttatcag tttcagcctt
      361 aaagataacg gtgccgaata tacaatgaca ttgcgaggca gtggatttga atacgaagaa
      421 atccccataa caataaatga gtataactca ttcatggatt tcaagaaccg tgaatttccg
      481 ttagaacaaa acagacgatt gtatgcctgg gatattctgc agaaaaaaca gtctgacata
      541 ccgaaaagga ttaaaggtta tattcaccag gcgattggtg acgtgtcttt aggttatgct
      601 ttattggatg atatagtaag caaattaaaa cggggtaaat ttgaactaca agggccagga
      661 ggtggaatta aacagtgcga tggatggtat atttacgaaa aaataattga tgataatttt
      721 gccatagtca tagaatcttt gggctttgcg cttaaaatct atggagggga tgaacgtttt
      781 cgtaatggtt cctctgttgt cttggaggat gaagattact ctcttattta taattttctg
      841 gttaatgcgg gttgccaaca agtcgaatta gccgaacaag tggatgcgat agtaagtgcg
      901 aatctggccg cagatagtga tattaccaaa gaaaagattt gcgagaaata taaatcgacc
      961 attgaggcgt ttaaaaaaga gcaactagcg ttaccagtat tagttcggcg taagaactca
     1021 gagacttaat tcccctatcc catagataac gataggggaa aattattttt gcagaattta
     1081 tgttattgcc attatttgag caacttttgc agcaggtcca tcacttgctg cacctcttcc
     1141 tgagtgagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag caccactaca
     1201 gcggaacttt cttcatccag ctgccaggca ccgcgcgcga cgccattgct atcgacaata
     1261 aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac aaacatgccg
     1321 gaacccggaa tcgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg atcgtgcggt
     1381 aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc agaggtgcga
     1441 ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt ccaggttttg
     1501 tagctaaact gatctttatc aagcaccaac tcgccccgat cggtaatgcc aatcggcggc
     1561 actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag caggcaggtg
     1621 agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca ggtgatccga
     1681 ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc aagtgtgatg
     1741 ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact gaagaccgcg
     1801 cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag ttatacgcgg
     1861 tggaaacatt gcccggatag tctatagtca ctaagcatta aaatttgcgc ctcataatag
     1921 ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca atgaaaattt
     1981 tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc cgtggacgat
     2041 tatacatcaa ggacgttggc gcttttgaat tcgataaggg taagattctt atcccaaaag
     2101 tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt atgcgtctgc
     2161 aaacagagat ggcttaacca aagtgctatg cagtaaaaag tgctatgcag taaaaagtgc
     2221 tatgcagtaa taagacggct cctgattcag gagccgttga tgtttatggg ggttacgcga
     2281 cgctttcttc tttatcttta gcgtcaggca gttttggcga aagggctgtg gccttgctgt
     2341 cgatccgggt caccagcagc tggtcgatgc ggtagttatc gatatccacc acttcaaatt
     2401 tgtagccggc gaatttcacc gaatcggtgc gtttcgggat cttacgcagc ataaacatca
     2461 taaagccgcc gatggtttcg tagttgcccg actgcgggaa ctcgtcaata tccagcacgc
     2521 gcatgacgtc gtcaatcgga gtgccgccgt caatcagcca tgagttctca tcacgggcga
     2581 caatctgctc ttccagtccc tgaccgacca gatcgcccat cagcgtggtc atcacgtcgt
     2641 tgagggtgat gatccccact accagcgcgt actcgttcat gatcaccgcg aagtcttctc
     2701 ctgcggtttt aaaactttcc aacgcctctg aaagggttaa cgtatccggc acaatcagcg
     2761 tgttgcgaat ttgtacgccg ctgttcagtg ccaggctttg gttagccagc acgcggttca
     2821 gcaggtcttt agaatcgaca tagccgatga tgtggtcaat atcttcatta cagacgagga
     2881 acttagagtg cggatgttcc gccaccttat ttttcagact ttgctcatct tcgtggagat
     2941 caaaccaaat cacgttttca cgcggtgtca ttgaagacgg aacggtacgg gattccagct
     3001 caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa cacgcccgcc agcgcaccgg
     3061 cttccactac cgcgtagatg tcatcagaag tgatgtcatc tttacgtacc attggcagtt
     3121 tgaaaatacg gaagattatg ttcgccaggc cgttgaagaa ccacaccagc ggggtgcaaa
     3181 cgtacaggca gaagcgcatc gggttgatga tacgcaaagc cacagcttct ggcgcaatca
     3241 taccgatgcg tttcggggtt aaatccgcaa acaggataaa catgccagtc actaacgaga
     3301 aagagagaat aaagctcagt tgctcagaga gctctgccga catatagcgg gagaacaggc
     3361 tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc gccgagaatc gccactgcgt
     3421 tcagaccgat ttggaccacg gtaaagaaca tgccgggatt ttcctgcata ttcagaacgc
     3481 gttgggcatt tatattgcct tcatcagcca gcagtttaag tttgattttg cgtgaggcgg
     3541 caagtgagat ctcagacatc gagaagaacg cacttacagc gatcaagcag agtatgacta
     3601 aaatactgtt taacatatct tatccgacct ttcaggccag atcctcggaa ggggaagttg
     3661 attaatttgt gtgaaataca cattgaaagc cgattcgaag aaagtgaacc ggcattttca
     3721 gtggctagta tagcgtaagg tactgtaaag ccgccagagg gttaaaattc agacagctga
     3781 aaaatgcaaa actgcctgat gcgctatgcc gatcaggcct acgcagtcct tgcaatatat
     3841 tgaatttgga cgattttgta ggcctggcaa ggcgtttacg ccgcatccgg cataaacaaa
     3901 acgcacttta tcaacaatct acatttgtac ccgtaacgct atgcttccgg tggcagacag
     3961 acgccaattc cgccaattcc acagtaaccg tacgggtttt tatgcagata ttgctggtgg
     4021 tcatcttcgg cataataaaa cggtgtggcg ttagcgattt ccgtggtgat gtgacgatcg
     4081 tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac tggcgcgagc tgcggcatcc
     4141 tgttctgggg tcagcggata aatcgctgaa cgatactgcg tgccgtggtc attgccctga
     4201 cgcattccct gggcgggatc gtgattctcc caaaatacct gtagcaactg ctcatagctg
     4261 atgacggaag gatcgtaaac aatgcgtacc gcttcggcat gaccagtatc accggagcac
     4321 acttcccgat aagtcggatt tggcgtatag ccgccggtat aacctgcggc ggtgctgtaa
     4381 acgccgtgta actgccagaa cagacgctcc acaccccaga aacaacccat cgcaaaaatg
     4441 gcaatctcca ttccgtcagg tacattggtc attgagtgac cgttaaccgc atgcagcgtg
     4501 gctacgggca tcggggtgtt acgtccaggc agggcatcgg cgggggaaac cagatgcttt
     4561 ttatcaaata aactcatggt gtcgctctcc cgaaaatcgg tcattggggt taaggttgta
     4621 acaagaggcg tatttgcaca caataaccac cgtgaatagg tctaaagtaa aacataagaa
     4681 atatttgggg tttagtctgc tttttaatcc atattactgg atttttgtta agccgtttaa
     4741 cggcgttcca ggggcaggaa aaaaggatat tcaggagaaa atgtgcgcta tatccgacag
     4801 ttatgctgtg taagcttact ctgcttaagc ggatctgccg tcgccgcgaa cgtccgtcta
     4861 caggtcgagg ggttatcggg acagctggaa aagaacgttc gtgcgcagct ttctacgatt
     4921 gaaagtgatg aagtgacgcc agaccgtcgc tttcgcgcac gcgtcgatga tgctatccgc
     4981 gaaggtctga aagcgctggg ttattaccag ccgaccattg aatttgatct ccgtccaccg
     5041 ccaaagaaag ggcggcaggt attgatcgcc aaagtcacgc caggcgtgcc ggtgttaatt
     5101 ggcggcaccg atgtggtatt gcgcgggggc gcgcggaccg ataaagacta tttgaaattg
     5161 ctcgatactc gcccggctat tggcacggta ctgaaccagg gcgattatga aaatttcaaa
     5221 aagtccttaa ccagcattgc gttgcgtaaa ggttatttcg atagcgaatt taccaaagcg
     5281 cagctgggca ttgcgctcgg cctgcataaa gccttctggg atattgatta taacagtggc
     5341 gaacgttacc gctttgggca tgtgaccttt gaaggatcac aaatccgcga tgaatacctg
     5401 caaaatctgg tgccgtttaa agagggcgat gagtacgaat cgaaagatct ggcagaactg
     5461 aaccgccgac tttctgctac cggctggttt aactcggtgg tggtggctcc acaatttgat
     5521 aaagcgcgcg aaacgaaagt attaccattg acgggcgtgg tttcgccgcg aacagaaaac
     5581 actatcgaaa ccggggtcgg ttactctacg gacgtgggac cgcgcgtgaa agcgacgtgg
     5641 aaaaaaccgt ggatgaactc atacggtcac agtctgacca ccagtaccag tatttccgcg
     5701 ccggaacaga ccctcgactt cagctataaa atgccgctgc tgaagaatcc actggaacaa
     5761 tattatttgg tgcagggcgg ttttaagcgc actgacctga acgataccga gtctgactcc
     5821 actacgctgg tggcttctcg ctactgggat ctctccagcg gctggcagcg tgccattaac
     5881 ctgcgctgga gtctcgacca ctttactcag ggtgaaatta ccaacaccac gatgctgttt
     5941 tatcctgggg tgatgattag ccgcacgcgt tctcgtggtg gcctgatgcc aacctggggc
     6001 gactcgcaac gctactctat cgactactcc aacacggcct ggggttcaga tgtcgatttc
     6061 tccgttttcc aggcgcagaa cgtctggatc cgcacactgt acgatcgcca tcgttttgtg
     6121 acacgcggca cgctgggctg gattgaaacc ggtgatttcg acaaagtacc gccggatctg
     6181 cgtttcttcg ccgggggcga tcgcagtatt cgcggctaca aatacaaatc tatcgccccg
     6241 aaatacgcta acggtgacct gaaaggggcc tcgaagttga taaccggatc gctggaatac
     6301 cagtacaacg tgaccggaaa atggtggggc gcggtgtttg tcgatagtgg cgaagcggta
     6361 agcgatattc gccgcagcga ctttaaaacc ggtaccgggg tcggcgtacg ctgggaatcg
     6421 ccggtcgggc caatcaaact cgattttgcc gtaccggtcg cggataaaga cgaacacggg
     6481 ttacagtttt acatcggtct ggggccagaa ttatgagttt atggaaaaaa atcagcctcg
     6541 gcgtggttat cgttatctta ctgttgctgg gatcggtggc gtttctggtg ggcaccacca
     6601 gcggcctgca tctggtattt aaagcggcgg atcgctgggt gccaggactg gatattggca
     6661 aggtcaccgg cggctggcgc gatctcacct tgtctgacgt tcgttatgag cagccaggcg
     6721 tggcggtaaa agcgggtaat ctgcatctgg cggtcggtct tgagtgcctg tggaacagca
     6781 gtgtttgtat taatgacttg gcgctgaaag acattcaggt caacatcgac agtaaaaaaa
     6841 tgcctccttc tgaacaggtt gaagaagagg aagatagcgg tccgctggat ctctccacgc
     6901 cgtatcccat caccctgaca cgggtggcac tggacaacgt caacatcaag attgatgaca
     6961 ccacggtatc ggtgatggac ttcacctccg gcctgaactg gcaggagaaa accctgaccc
     7021 tgaaaccgac gtcgctgaaa ggcctgctga ttgctctgcc gaaagtggcg gaagtggcgc
     7081 aggaagaagt ggtcgaaccg aaaattgaaa atccgcagcc ggaggaaaag cctctcggcg
     7141 aaacactcaa agatctcttt tctcgcccgg tattgccgga aatgaccgac gtgcatctgc
     7201 cactcaacct gaacattgaa gagtttaagg gagagcaact gcgcgtgacg ggcgatacgg
     7261 acattaccgt gcgcaccatg ctgctgaaag tgagcagcat tgacggcaat actaaactgg
     7321 acgccctgga tatcgattcc aatcaaggga tcctcaacgc cagcggcacg gcgcagctgt
     7381 cagacaactg gccggtggat atcaccctca acagcacact gaacgtggag ccgttgaaag
     7441 gtgaaaaggt gaagctgaaa gtgggcggcg cgctgcgcga acagctggag attggcgtta
     7501 atctttccgg tccggtggat atggatttac gcgcccagac gcgactggcg gaagccggat
     7561 tgccgctcaa cgtggaagtg aacagcaaac agatttactg gccgttcact ggtgagaagc
     7621 agtatcaggc ggatgatctg aaactgaaac ttaccggcaa aatgaccgat tacacgctct
     7681 ctatgcgtac ggcagtgaag ggactggaga tcccgccagc caccattacc cttgatgcca
     7741 aaggtaatga acagcaggtc aatctcgaca aactcaccgt cgcggcgctg gaagggaaaa
     7801 ctgaactcaa ggcgttgctt gactggcagc aggccattag ttggcgcggt gagctaacgc
     7861 ttaacggcat taacaccgcc aaagagatcc cagagtggcc gtcgaaactc aatggcttga
     7921 ttaaaacccg cggtagcctg tacggcggca cctggcagat ggaggtgcca gaactgaagc
     7981 ttaccggtaa cgtcaaacag aacaaagtga acgttgacgg cacgctgaaa ggcaacagtt
     8041 atatgcagtg gatgatccca gggcttcatc tggaactggg gccaaacagt gccgaagtga
     8101 aaggcgagct gggggtaaaa gatctcaatc ttgatgccac catcaacgcg ccggggctgg
     8161 ataacgcgct gccggggctt ggcggtacag cgaaagggct ggtgaaagtt cgcggcacgg
     8221 tggaagcgcc acaactactg gcggacatca ccgcgcgcgg cctgcgctgg caggagctaa
     8281 ccgtggcgca ggttcgcgtg gaaggcgata tcaaatccac cgatcagatt gcgggtaaac
     8341 tcgacgtgcg cgttgagcaa atttcacagc cggatgtaaa tatcaacctc gtcaccctga
     8401 atgccaaagg cagcgaaaaa cagcacgagt tacagttgcg gattcagggc gagccggttt
     8461 ccgggcagct taatctggca ggaagttttg atcgcaaaga agaacgctgg aagggaactc
     8521 ttagcaatac ccgcttccag acgctggtcg gcccgtggtc gctgacccgc gatattgcgc
     8581 tggattaccg caataaggag caaaaaatca gcatcgggcc acactgctgg cttaacccga
     8641 atgcggaact gtgcgtgccg caaactatcg atgcgggggc cgaagggcgt gcggtggtga
     8701 atctcaaccg cttcgacctt gccatgctga aaccgtttat gccagaaacc actcaggcca
     8761 gcggtatctt cacgggtaaa gcggacgttg cctgggacac caccgaagag gggctgccgc
     8821 agggcagtat caccctttcg gggcgtaacg tgcaggtaac gcaaaccgtc aacgatgcgg
     8881 cgctgcccgt ggcgtttcag acactgaatc tgacggcgga attgcgtaac aaccgtgccg
     8941 aattgggctg gaccatccgc ctgaccaata acggccagtt tgatggacag gtgcaggtga
     9001 ccgatccgca aggccgccgt aatcttggtg gcaacgtcaa tatccgtaac ttcaaccttg
     9061 cgatgataaa ccccatcttt acccgtgggg aaaaagcagc ggggatggtg agtgccaact
     9121 tgcgtctggg tggtgatgtg caaagcccgc agttgtttgg tcagcttcag gttacgggtg
     9181 tggatatcga tggcaacttt atgccgtttg atatgcaacc gagccagctt gcggtcaact
     9241 ttaacggtat gcgctcgacg cttgccggta cagtacgaac ccagcagggt gaaatctacc
     9301 tgaacggtga tgccgactgg agccaaattg aaaactggcg ggcgcgggta acggcgaaag
     9361 gcagtaaagt gcggatcacc gtgccgccga tggtacgaat ggatgtatcg ccagatgttg
     9421 tattcgaggc tacaccaaac ctgtttaccc tcgatggtcg cgtggatgtc ccttgggcgc
     9481 gcatcgtggt gcacgatctg ccggaaagcg cagtaggcgt ctccagcgat gtggtgatgc
     9541 ttaacgataa cctgcaaccg gaagagccga aaacggcgtc gattccgatt aacagtaacc
     9601 tgattgtcca cgttggcaac aatgtgcgca ttgacgcctt tggcctgaaa gcgcggctga
     9661 cgggcgatct taacgtcgtt caggacaaac aagggctggg cctgaacggg cagatcaaca
     9721 tccctgaagg gcgcttccat gcctatggtc aggatctgat tgtgcgtaaa ggtgagttac
     9781 tgttctctgg cccgccggat caaccgtatc ttaatattga agctattcgt aacccggatg
     9841 ctacagaaga cgacgtaatc gccggagttc gcgtcactgg tctggcggac gaaccgaaag
     9901 cggagatctt ctctgacccg gcgatgtcgc aacaagctgc cttgtcttat ttgctacgtg
     9961 gacaagggct ggagagcgat cagagcgaca gtgcggcaat gacctcgatg ctgattggtc
    10021 tgggggttgc gcaaagtggc cagattgtag gtaaaatcgg cgagacgttt ggcgtaagca
    10081 atttagcgct cgacacccag ggagtaggcg actcctccca ggtagtggtc agcggctatg
    10141 tattgccagg tctgcaagtg aaatacggcg tgggtatatt tgactctata gcaacactca
    10201 cgttacgtta tcgcctgatg cctaagctat atctggaagc cgtgtctggt gtagaccagg
    10261 cactggattt gctctatcag ttcgagtttt agcaatgcga atatttgtct acggcagttt
    10321 acgccacaaa caaggcaaca gtcactggat gaccaatgcc cagttactgg gcgatttcag
    10381 tatcgataac taccagttgt atagcctggg ccactatcca ggcgcagttc cggggaacgg
    10441 aacggtacac ggtgaagttt atcgtattga caacgccacg ctggccgaac ttgatgcctt
    10501 gcgcaccagg ggcggtgaat acgcgcgcca gttgattcag acgccgtacg ggagtgcatg
    10561 gatgtacgtt tatcaacgac ccgtcgatgg attaaagcta attgaaagcg gcgactggtt
    10621 agacagggat aagtaaccct atgcatacgc caccttcggg tggcgttgtt ttttgcgaga
    10681 cgactcgcat tctgttttgt aattccctca ccttttgctt ttctctccga gccgctttcc
    10741 atatctatta acgcataaaa aactctgctg gcattcacaa atgcgcaggg gtaaaacgtt
    10801 tcctgtagca ccgtgagtta tactttgtat aacttaagga ggtgcagatg cgtattacca
    10861 taaaaagatg ggggaacagt gcaggtatgg tcattcccaa tatcgtaatg aaagaactta
    10921 acttacagcc ggggcagagc gtggaagtgc aggtgagcaa caaccaactg attctgacac
    10981 ccatctccag gcgctactcg cttgatgaac tgctggcaca gtgtgacatg aacgccgcgg
    11041 aacttagcga gcaggatgtc tggggtaaat ccacccctgc gggtgacgaa atatggtaaa
    11101 gaaaagtgaa tttgaacggg gagacattgt gctggttggc tttgatccag caagcggcca
    11161 tgaacagcaa ggtgctggtc gacctgcgct tgtgctctcc gttcaagcct ttaatcaact
    11221 gggaatgacg ctggtggccc ccattacgca gggtggaaat tttgcccgtt atgccggatt
    11281 tagcgttcct ttacattgcg aagaaggcga tgtgcacggc gtggtgctgg ttaatcaggt
    11341 gcggatgatg gatctacgcg cccggctggc aaagcgtatt ggtctggctg cggatgaggt
    11401 ggtggaagag gcgttattac gcttgcaggc ggtggtggaa taaggtgtgt ttatttatcg
    11461 cgggcataaa aaaaccctta ctaaccgaag cccggcgttc agggttatta cgccagaaga
    11521 acttatttat tctttgcgcg ctcgaaggag gcaacgattt cagctttagc ggcttctgcg
    11581 ttttcccaac cttcaacttt cacccacttg cctttttcga ggtctttgta gtgctcgaag
    11641 aagtgagcga tttgcgcttt cagcagttca ggcagatcgt taacgtcttt aatgtgatcg
    11701 tattctttgc tcagcttgct gtgcggaacc gcaaccagtt tcgcatcttc accggcttcg
    11761 tcggtcattt tcagaacgcc aaccggacgg caacggatca cagaacccgg ctgcagcggg
    11821 tacggagttg ggaccagtac gtcaactggg tcaccgtcca gagacagggt gtggttgatg
    11881 taaccgtagt tgcacggata gaacatcgcg gtggacatga agcggtcaac gaacagtgcg
    11941 ccgctctctt tgtcgatttc gtatttgatc ggatctgcgt tagccgggat ctcaataaca
    12001 acgtagatgt cttccggcag atctttaccc gcagggacgt tgagtaagct catgtctgtt
    12061 tcctttaaaa atatgtggca aacaagtgcc gagtattata gccaactcgc gccgaatgtc
    12121 ttcgcttgtt ttcgtcttca ttttcctttt tcgccagttt taagacggta tgcagagcag
    12181 gaaaatccat aaccgtagcc gcatttttca tagtgagatg aaagcgatta caaacttgtg
    12241 attaacgttt tatttacttt tttgaagtgt gatgtaacgc aatccgttac ataacgaatt
    12301 gtctatagtt ttttcgcgaa catcttttca ccaataataa ttactccgac gaggacaacc
    12361 ctatgtggaa acgcttactt gtagtctctg cagtctcggc agccatgtcg tctatggcgt
    12421 tggccgctcc attaaccgta ggattttcgc aggtcggatc ggaatccggc tggcgcgccg
    12481 cagaaaccaa tgtggcgaaa agtgaggccg aaaaacgcgg aattacgctg aaaattgccg
    12541 atggtcagca aaagcaggaa aaccagatta aagcggtacg ttccttcgtt gcacaagggg
    12601 tggatgcgat ctttatcgct ccggtggtcg cgacaggttg ggaaccggta ttaaaagagg
    12661 cgaaagatgc cgaaatcccg gtcttcttgc tcgaccgttc catcgatgtg aaagacaaat
    12721 ctctctatat gaccaccgtt actgccgaca acatcctcga aggtaagttg atcggtgact
    12781 ggctggtaaa agaagtgaat ggcaaaccat gcaacgtggt ggagctgcag ggcaccgttg
    12841 gggccagcgt cgccattgac cgtaagaaag gctttgccga agccattaag aatgcgccaa
    12901 atatcaaaat catccgctcg cagtcaggtg acttcacccg cagtaaaggc aaagaagtca
    12961 tggagagctt tatcaaagcg gaaaacaacg gcaaaaacat ctgcatggtt tacgcccata
    13021 acgacgacat ggtgattggt gcaattcagg caattaaaga agcgggcctg aaaccgggca
    13081 aagatatcct cacgggttcc attgacggtg taccggacat ctacaaagcg atgatggatg
    13141 gcgaagcgaa cgccagtgtt gaactgacgc cgaacatggc gggacccgct ttcgatgcgc
    13201 tggagaaata caaaaaagac ggcaccatgc ctgaaaagct gacgttaacc aaatccaccc
    13261 tttacctgcc tgataccgca aaagaagagt tagagaagaa gaaaaatatg gggtattgag
    13321 ggttgctatg cctgatgccg attcgtaggc cggataaggc gctcgcgccg catccggcga
    13381 tggtgcactg aagcctgatg cgacgcttac cgcgtcttat catgcctact gggagcacgc
    13441 tttacaccgg gggaaaccat gacgaccgac caacaccagg agatcctccg caccgaagga
    13501 ttaagtaaat ttttccccgg cgtcaaagcg ttagacaacg ttgatttcag cctgcgccgt
    13561 ggcgaaatca tggcgctgct cggtgaaaac ggggcgggaa aatcaacgct aatcaaagca
    13621 ttaactggtg tataccacgc tgatcgcggc accatctggc tggaaggcca ggctatctca
    13681 ccgaaaaata ccgcccacgc gcaacaactc ggcattggca ccgtctatca ggaagtcaac
    13741 ctgctaccca atatgtcggt cgctgataat ctatttatag gccgcgaacc caaacgcttc
    13801 ggccttctac gccgcaaaga gatggaaaag cgcgccaccg aactgatggc atcttacggt
    13861 ttctccctcg acgtgcgcga accgctcaac cgcttttcag tcgcgatgca gcaaatcgtc
    13921 gctatttgcc gggctatcga tctctctgcc aaagtgctga tcctcgatga acccaccgcc
    13981 agtctcgaca cccaggaagt ggagttactg tttgacctga tgcgtcagtt gcgcgatcgc
    14041 ggcgtcagcc tgatctttgt cactcacttt ctcgatcagg tctatcaggt cagcgatcgg
    14101 atcaccgtct tacgcaacgg cagtttcgta ggctgtcggg aaacgtgcga gctaccgcag
    14161 atcgaactgg taaaaatgat gctggggcgc gagctggata cccacgcgct acagcgtgcc
    14221 gggcgaacat tgttgagcga caaacccgtt gccgcgttca aaaattacgg caaaaaagga
    14281 acgatcgcac cgtttgatct cgaagtacgc cccggcgaga tcgtcggtct ggctggattg
    14341 ctgggatcag gacgtaccga aaccgccgaa gtgatcttcg gtatcaaacc tgctgacagc
    14401 ggcacggcgt tgatcaaagg caaaccgcaa aacctgcgat cgccacatca ggcttcggta
    14461 cttggcattg gcttctgccc ggaagacagg aaaaccgatg gcatcatcgc tgccgcctcg
    14521 gtgcgggaaa atatcatcct cgctctccag gcccagcgcg gctggctacg tcccatttcc
    14581 cgcaaagaac agcaagagat tgccgaacgc tttatccgcc agcttggcat tcgcacacct
    14641 tcaactgaac aaccgattga atttctctcc ggcggcaatc agcaaaaagt gttgctttca
    14701 cgttggctac tgacccgacc gcaatttctg atcctcgatg agccaacccg cggcattgat
    14761 gttggtgccc acgccgagat catccgcctg attgaaacgc tatgcgccga tggtctggcg
    14821 ctgctggtga tctcctccga actggaagaa ctggtgggct atgccgaccg ggtgatcatc
    14881 atgcgcgatc gcaaacaggt ggcggagatc ccgctggcag agctttccgt tccggcgatc
    14941 atgaatgcca ttgcggcgta aggagaacag tgtgatgcct caatctctcc cggacactac
    15001 gccgccaaaa aggcgctttc gctggccaac gggaatgccg cagctggcgg cactgttgct
    15061 ggtgctgctg gtcgatagcc tggtggcccc gcatttctgg caggtggtgc ttcaggatgg
    15121 gcgtttgttc ggtagcccta tcgacattct taatcgcgcc gcccccgttg cgctactggc
    15181 gattggtatg acgctggtga tcgcaacagg tgggattgat ctctccgtag gggcggtgat
    15241 ggctatcgcc ggagccacaa cggctgcgat gacggtcgcg ggattcagcc tgccgattgt
    15301 tttgttaagc gccctgggca ccggcatcct ggcgggattg tggaacggca tactggtagc
    15361 gatcctcaaa attcagccgt ttgtcgccac tctgatcctg atggtcgccg ggcgcggcgt
    15421 ggcgcaactg atcacctccg gacagatcat cacgtttaac tcgccggatc tctcatggtt
    15481 cggcagtgga tcgctgttgt tcctgccaac gccggtcatt atcgcggtgc tgacgcttat
    15541 cctgttctgg ctgttgaccc gcaaaacggc gctggggatg tttatcgaag ccgttggtat
    15601 caacattcgg gcggcaaaaa atgccggggt aaacacgcgg atcatcgtca tgcttaccta
    15661 cgtgttgagc gggctgtgtg cggcgattgc gggcattatc gtggcggcgg atattcgcgg
    15721 tgccgatgcc aacaacgccg gattatggct ggagctggac gccattctcg cggtggtgat
    15781 tggcggtggt tcgctgatgg gcggacgttt taacctactg ctttcggtgg tgggggcgct
    15841 gattattcag gggatgaaca ccggaatttt gctttcgggc tttccgccag agatgaacca
    15901 ggtggtgaaa gcggtggtgg tgctttgcgt gctgattgtc cagtcgcaac gctttatcag
    15961 tctgattaaa ggagtgcgta gccgtgataa aacgtaattt gccactgatg atcaccatcg
    16021 gcgtctttgt gctgggttat ctctactgcc tgacccagtt tcccggtttt gcttccacaa
    16081 gagtgatctg caatatcctg accgataatg cctttcttgg gataatcgcc gttggcatga
    16141 cctttgtgat cctctccggt gggatcgatc tctccgttgg ttcggtgatc gcctttaccg
    16201 gcgtgtttct ggcaaaagtg attggcgatt tcggcctctc gccgttgctg gcatttccgc
    16261 tggtgctggt aatgggctgt gcctttggcg catttatggg gcttttgatc gacgccctga
    16321 agatcccggc atttatcatt actttggcag gaatgttctt tttgcgcggc gtcagctacc
    16381 tcgtttcgga agagtcgatc ccgataaacc atcctgttta tgacacgctc tcaagccttg
    16441 catggaaaat tcctggcggc ggtcgcttaa gtgcgatggg gctgctgatg ctggcggtgg
    16501 tggttatcgg catattcctc gcgcatcgta cccgctttgg taatcaggtg tacgccattg
    16561 gcggcaatgc gacgtcggca aacctgatgg ggatttccac tcgcagcaca actattcgca
    16621 tttatatgct ctctaccgga ctggcgacac tggcggggat tgtcttctcg atttataccc
    16681 aggccggata cgcgctggcg ggcgtaggtg tggaactgga cgctatcgcc tcagtggtga
    16741 ttggcggtac gcttttgagc ggtggcgttg gaacggtatt agggacgctt tttggcgtgg
    16801 cgattcaggg actgattcag acttacataa actttgatgg tacgctgagc tcatggtgga
    16861 cgaaaatcgc catcggtatt ttgttgttta tttttatagc attacagcgt ggattaacgg
    16921 tgctgtggga gaatcgtcag agttcgcctg tgacaagagt caacattgcg cagcaataaa
    16981 atcgcctctc cgtgtggaga ggcgcaggag attacgcgtc cgggaactca cagataaagc
    17041 gttcgacatc ttcaaccata tggtcgttgc cgacaaagaa tgaacggcgc tggtgcaggg
    17101 tttccgggat aatatccaga atacgctctt tgccatcgct cgctttaccg cccgcttgtt
    17161 ccgccaggaa tgccatcggg ttgcactcat acagcaaacg cagtttgccg tccgggtggc
    17221 tggcggtgct tgggtagaga taaataccgc ctttcagtag gttacggtgg aaatccgcaa
    17281 ccagtgagcc gatataacgt gaggtatacg ggcggttggt ggatttatct tcttcctggc
    17341 agaatttaat gtacttcttc accccgttcg gaaacttaat gtagtttcct tcgttgatgg
    17401 agtaggtttt gcctttctcc gggaagcgca tccgttcctg gcacaggcag aaaacgccga
    17461 gcgaaggatc gtaagtaaaa gcgtgaacac cgcatccggt actgtaaacc agcatggtag
    17521 aggagccgta taccacgtaa cctgccgcaa cctgtttgtt accaggctgg aggaaatctt
    17581 cttccgttac cggcgtgccg acgggcgtaa ctcggcggta gatggagaaa atggtaccga
    17641 cagagacgtt aacatcgatg ttggacgagc catccagtgg gtccatcagc accacgtatt
    17701 ttgcatgttc acagccttca aagacaacaa tctcatcttc ttcttcagag gcaatgcctg
    17761 caacgatatc gcgtgctttc agtgcggctt tcagtttttc attagcgaac aagtcgagtt
    17821 tttgctgaac ctcgccctgc acgttctcag caccgctggc acccaggata tcaaccagtc
    17881 ctgctttgtt gatatcgcga tggataatct tggcgcccag ttttattgcc gacagcaaag
    17941 cagtgagctc accggtagca tgagaaaact cgtgctgctt ttcgacaata aattcaccta
    18001 acgttttcat aaaactttcc ctgcaatgtt tatggagtaa agcgaccgca acaatcttaa
    18061 caaataatct caatgttgcg ctcaggtgaa tcgcgccagc aaattacgga ttatcctgaa
    18121 atgcgtttct cacttgcccg acatatgcgt aaaatgagcg gcagattaaa aaaggatagt
    18181 gacgcatgcg cattcatatt ttaggaattt gtggcacgtt tatgggcggt ctggcgatgc
    18241 tggcgcgcca gttaggccat gaagtaacgg gttcggacgc caatgtgtat ccgccgatga
    18301 gcaccttact tgagaagcaa ggcattgagc tgattcaggg ttacgatgcc agccagctcg
    18361 acccgcagcc ggatctggtg attattggca acgccatgac ccgtggaaat ccgtgtgtgg
    18421 aagcggtact ggaaaaaaac atcccttata tgtcaggtcc acagtggctg cacgattttg
    18481 tgctgcgcga ccgctgggtg ctggccgttg ccggtacaca cggcaaaacc accaccgcgg
    18541 gaatggcgac ttggattctg gaacagtgtg gttacaaacc gggctttgta atcggcggtg
    18601 tgccggggaa ctttgaggtt tcggcgcgtc tgggcgaaag cgacttcttt gttatcgaag
    18661 cggatgagta cgactgcgcc ttcttcgaca aacgctctaa atttgtccat tactgcccgc
    18721 gtacgctgat cctcaacaac cttgagttcg atcacgccga tatctttgac gacctgaaag
    18781 cgatccagaa acagttccac catctggtgc gtatcgttcc ggggcagggt cgtattatct
    18841 ggccggaaaa tgacatcaac ctgaaacaga ccatggcgat gggctgctgg agcgagcagg
    18901 agctggtggg tgagcagggt cactggcagg cgaaaaagct gaccaccgat gcttccgaat
    18961 gggaagtttt gctggatggc gaaaaagtgg gcgaagtgaa atggtcgctg gtaggcgaac
    19021 ataatatgca caatggcctg atggcgattg cagcggctcg ccatgttggt gtagcgccgg
    19081 cagatgccgc taacgcgctg ggttcgttta ttaacgctcg ccgccgtctg gagttgcgtg
    19141 gtgaagcgaa tggcgtcacg gtatatgacg attttgccca tcatccgacg gcgattctgg
    19201 caacgctggc ggcgctgcgt ggcaaagttg gtggtacggc gcgcattatt gctgtgctgg
    19261 agccgcgctc gaataccatg aaaatgggga tctgcaaaga cgatctggca ccttcattag
    19321 gtcgtgccga tgaagtcttc ctgctgcaac cggcgcatat tccgtggcag gtggcagaag
    19381 tggcagaagc ctgcgttcag cctgcacact ggagtggcga tgtggatacg ctggcagata
    19441 tggtggtgaa aaccgctcag cctggcgacc atattctggt gatgagcaac ggcggttttg
    19501 gtgggatcca tcagaaacta ctggatggtc tggcgaagaa ggcggaagct gcgcagtaat
    19561 tcggcctcag cctgagatag tgtgcgtagg ccggataagg cgttcacgcc gcatccggca
    19621 tttgagcata gtgcctgatg cgacgcttga tgcgtcttat caggcctaca ggtgcaccgc
    19681 atccggcaaa ccatactcac atcaacaacg aaaattaccc ttcattctcc gccagttcgc
    19741 gcagatactg gaaaatctgg cgtgcggatt tcggcggctt attcccttct ttctctttct
    19801 tcgcgttgcg gatcagagta cgcagttgct gacgatcggc atcgggccac agatttaata
    19861 cctcagcgat ggcgtcatca ccttgatcga tcagacgatc gcgcaggttt tccagtttat
    19921 ggaacagcac cacctgctgg ttgtgacggt ttttcagctt atccagcgcc tgacggatcg
    19981 gttctacatc gcgctggcgc agcatcttac cgatgagctg caactggcgg cggcgacctt
    20041 ccatcttaat acgctgggcg agctcaatag ccgcgcgcag atccgcatct aacgggatct
    20101 tatccagcgc gtttttcccc agatcaacaa tttccgcgcc aaggcgtttt agctcctcgg
    20161 catcacgttt aatttcactt ttactgaccc agataatttc atcgtcttcg tcttcgatgt
    20221 catcaccggg aacgtcgtcg agccagtctt cgggctgctt agtcatctca ggctccttaa
    20281 aaaaagaggc taatgttacc agttaagatg cgcactgaaa aacggttctc tgttagactt
    20341 cagagaaact ctctacatta tggcacttgc aatgaaagta atctctcaag ttgaagcgca
    20401 gcgcaagatt ctggaagaag cagtttccac tgcgctggag ttggcctcag gcaaatcgga
    20461 cggtgcggaa gttgccgtca gcaagaccac cggcattagc gtaagcacgc gttatggtga
    20521 agtggagaat gttgaattca atagcgatgg cgcgctgggg atcactgttt atcaccagaa
    20581 ccgcaaaggt agcgcatcat ccaccgattt aagcccgcag gccattgccc gtactgtaca
    20641 ggcggcgctg gatattgccc gttatacctc gccagatccc tgtgccggcg tggcagacaa
    20701 agagctgctg gcctttgacg caccggatct cgacttgttc caccctgcgg aagtttcccc
    20761 ggatgaagcc attgaactgg cggcccgcgc agaacaggcg gcattgcagg cggacaaacg
    20821 catcaccaat accgaaggtg gcagctttaa cagccactac ggtgtcaaag tttttggcaa
    20881 cagccacggc atgttgcagg gttactgctc aacgcgtcat tcgctctcca gctgtgtaat
    20941 tgccgaagaa aatggcgata tggagcgtga ttacgcctac accattggcc gcgcgatgag
    21001 cgatttgcag acgccagagt gggtcggggc cgattgcgct cgccgtactt tatcgcgtct
    21061 gtcaccgcgt aaactctcca ccatgaaagc gccggtcatt tttgccaatg aagtggcaac
    21121 cgggcttttt gggcatctgg tgggggcgat agcgggtgga tcggtttacc gtaaatctac
    21181 cttcctgctg gattcgctgg gtaaacaaat tctgccggac tggctgacca ttgaagagca
    21241 tccgcatctg ctgaaagggc tggcatcgac gccatttgac agcgaaggtg tgcgcaccga
    21301 gcgtcgcgat attattaaag atggcatcct gactcagtgg ctgctgacca gctactcggc
    21361 gcggaaactg gggctgaaaa gcaccggaca tgcgggcggt attcacaact ggcggattgc
    21421 cggacaaggt ctaagcttcg agcagatgct caaagagatg ggcaccgggc tggtggtgac
    21481 ggaattgatg ggccagggcg tgagtgcaat taccggtgat tattcccgtg gtgcggcggg
    21541 cttctgggta gaaaacggcg aaattcagta tccggttagc gaaatcacca tcgcaggtaa
    21601 tttaaaagat atgtggcgca atattgtcac cgtcggtaac gatattgaaa cacgcagtaa
    21661 tatacagtgt ggttctgtgc tgttgccgga gatgaaaatc gccggacagt aaaaaaactg
    21721 gcgcgacctg ccgcgctata aactaaaaaa ttacacaaaa tcattcgcac tgcatcgaga
    21781 cggaaagtga atgagggccg gggagcgtac tcgcagtacg tgacccggtc gaatgagcgt
    21841 agtcgtcgat gaggcagtgt gaaggatgaa gtgtaaataa aaaaggaagt gagcaatgcg
    21901 aaaaagcctg ttagctattc ttgcagtctc ctcgttggta ttcagttcgg cgtcgtttgc
    21961 tgctgatctt gaagacaata tggaaaccct caacgacaat ttaaaagtgg tcgaaaaagc
    22021 cgataacgcg gcgcaagtca aagacgcgtt aacgaagatg cgcgccgcag cgctggatgc
    22081 gcaaaaagca acgccgccga agctcgaaga taaatcaccg gacagcccgg aaatgaaaga
    22141 tttccgccac ggtttcgaca ttctggtcgg tcagattgac gacgcgctga agctggcaaa
    22201 ttaaggtaaa gtaaaagaag cgcaggctgc tgcagagcaa ctgaaaacga cccgcaattc
    22261 atatcacaag aagtatcgtt aattcctcat ttccctgttg cctgcactca ggtaacaggg
    22321 aacttatcat cgcaaatgat gcaccacctg attgctgctg ccgcgccaga tcagggaagg
    22381 gtcttttaaa tcctgcacaa atttgccgtc gaccagcacg ttaatcaaat caacaacctg
    22441 catttgcgca gcgttgagtt cgtcgagttt atagcctgtc cacacccaga tgtctttacc
    22501 cggacactcg gcgcggatgc gttgtaccag tttcagaata tccggcacgt tttgcggatg
    22561 cagcggatcg ccgccggaga gggaaatacc ctggcgtttg atacgagtgt cattcagatc
    22621 gttaatgatc tggtcttcca ttgctttagt aaatggctga ccggaattta cccgccatgt
    22681 gcttttgtta tagcaaccgg ggcattcatg aacacacccg gagacaaaca gggtgcagcg
    22741 agtgccggga ccgttgacga tgtcgacagg atagtactga tgataattca ttatttcgcc
    22801 tggcttatca gtccgcgtgt aggctgtatc aggcaatcaa cgcctgatgc gacgctgtcg
    22861 cgtcttatca ggcctacagc cgttgccaaa tgtaggccgg ataaggcgtt tacgccgcat
    22921 ccggcaatca acgcgggatg cgacgctttc acatcacatc cggcatttgc ttagtgcaga
    22981 tcaacctatc tgcccatttc ccaaatgttt aacgcggcgc ttaacttctt cctgcttgcc
    23041 ggcgttaaac ggacgtgcat ccgggctacc taaatatccg cacacgcggc gagttaccga
    23101 cacacgggag gcgtcatggt taccacattt cgggcaggtg aagcctttgc tggtgcactc
    23161 gaactcaccg gtaaagccac actcgtagca ctcatcaatc ggtgtattgg tgccgtaata
    23221 cggtacatgc tgatagctgt aatcccagac atcttccagc gccttcaggt tgtgctgaat
    23281 gtttggatac tcgccgtagc aaatgaaacc accgttcgcc agcggcgggt aaggcgcttc
    23341 aaagtcgatc ttgtcgtacg ggttcacctt cttctccaca tcgaggtgga aactgttggt
    23401 gtagtaacct ttatcggtca cgcccggcac cacgccaaac tcagcagtat cgagacggca
    23461 gaagcggtcg cacaggtttt cactcggcgt gctgtagaga ctgaaaccat aacccgtttc
    23521 ttctttccac tgatccactg cctgacgcag acgttcaaca atggcgatac ctttcgcgcg
    23581 gagctgctcg ttgtcgtaaa catgttcgcc gccgaacagc gcgttaatgg tttcgtggat
    23641 gccgatgtaa cccagcgaaa tagacgcacg accgtttttg aagatttcag aaatatcatc
    23701 gtcagcattg agacgcacgc cacaagcacc ttccatatag aggatcgggg ccacgcgcgc
    23761 tttcacgcct tcgagacgag cgatacgggt catcagagcc ttacgtgcca gcaccagacg
    23821 ttcatccagc agcttccaga aggtggcttc atcgcctttt gcttccagag caatacgcgg
    23881 caggttcagg ctgatcacgc cgaggttgtt acgaccatcg tggatctgct cgccgttttc
    23941 attttcccac acgccgagga agctgcggca gcccatcggg gttttaaacg aaccggtgac
    24001 tttcactacc tgatcgtagt tcaggatatc cggatacatg cgcttgcttg cgcactccag
    24061 cgccagctgt ttgatgtcgt agttcggatc gcctttttta tggttcagac catcgcgaat
    24121 cgcaaacacc agtttcggga acaccgcagt tttacggttt ttaccgaggc ctgcgatacg
    24181 gttacgcagg atagattcct gaatcaggcg cgactcccag ctggtgccca gaccaaaacc
    24241 aaaggttaca aacggcgtct gaccgttggc ggtgtgcagg gtgtttactt cgtactccag
    24301 cgactggaag gcatcgtagc actctttaat ggtacgagag ttagcgtagc cttcggcgtc
    24361 cgggatgttc cactcttctg cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg
    24421 tgccagcact tcatcgatac ggttaatggt ggtgccgcca taaatatggc tggcaacctg
    24481 agcaataatc tgcgcagtta ccgcggttgc cgtagagatc gacttcggcg gttcaatctc
    24541 ggcgttcccc atcttaaagc cctgggtcag catgcctttc aggtcgatca acatgcagtt
    24601 gaacatcggg aaaaacggtg agtaatcgag atcgtgatag tgaatatcgc cacgctcatg
    24661 tgcctgcacc acgtcacgcg gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc
    24721 ggccagcagg tcgcgctggg ttggaatcac cttgctgtct ttgttggcgt tttcgttgag
    24781 taacgaggcg ttggtctgct cgaccagacc acggatctcc tgattcaggc gaccgcgttt
    24841 ttcacgttca atgtcgcgat cgtgacggta ctcgatgtaa gcacgagcca gttgtttgta
    24901 tggacctgac atcagctggt tttcaacagc ggtctggatc tcattgatat ccacctggtt
    24961 gcggccctgc atctgctcgc tgacaactgc agcaacagtg gcgcaataat cggcatcatc
    25021 gacttccgct gctttagctg cacgcagaat cgcttctttg atgcgctctg atttaaacgg
    25081 cactttgcag ccgtctcgtt tcatcacatg cggtgtcatg atcgctccat atttttaaga
    25141 acaggttatc cacagaaatt gggaaaggcg ttttccggtt ttttcgtttc gttttccggt
    25201 gctttccgca atcgccatcc gcttatccac aacgccgcac ctttttcgtg gggagctgta
    25261 gtagcaatta tagtcgattg atacaacata ttgggttggg acgcatttta aagtctatat
    25321 atagtgcttt gcatcaagga tgtttgaact ttttttgatg tagctcaaag taaaaagcag
    25381 agcgtacgga tgacgggcgc tacagcgtta tgtaaatttt ttaatgaatt tgctggttga
    25441 aaaatcaaca aaaacaacat gctgacagac aaaaccccgg aatgaccggc attaccgggg
    25501 cttagggaag atttacttct gtaaccacca gacagcctca aaaggccgta aagtcatggc
    25561 acagggttgt ggtgaggctt cttcgtagtt atgcatcaca agctgccagt tgccgcgcat
    25621 ttgccctggc tgccagggtt ggatctcacg gctaaggttg gcaatgacca gcaaggtttg
    25681 ccccttccat tcacggcgat agcaccacaa tacagggctg tttggcagca gatcctggta
    25741 attgccccat gtcaggatgg cttcctgctt acgcagtgcg attaactttt ggtaggtgta
    25801 aaacaccgag gactcatcgg ccagcgcggc ttctacgttg atttgttgat agttatcgcc
    25861 caggccaatc cacggttcgc cagccgtaaa cccggcatta tcgccgttgc tccattgcat
    25921 gggcgtgcgg ctgttgtcac gggatttact ggcgaggatt gccaataact cgtcggcatc
    25981 acgcccatcg ttgcgcagct cggcaaacat attgaggctc tctacgtcgc gatagtcagt
    26041 aatgcgcgtg aaatgcgggt tggtcatgcc aatctcttcg ccctggtaga tatacggcgt
    26101 tccctgcatg ccatgcagca ccatcgccag catttttgcc gcaggcacgc ggtattcacc
    26161 ttcatcacca aagcgagaaa caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt
    26221 caatgctacg ttgtgcattc cttgttgcca gtggcggaac aatgttttca acgccacaaa
    26281 gtcaggttta gccagcgtcc atttttcacc accgggataa tcgaccttca ggtgatgaaa
    26341 attaaaggtc atcgacaatt cactgcctgt cagtgccgcg tatcgctggc aatgctcaag
    26401 gctggtggag gacatttcac ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt
    26461 catctcgtgc aaaaactcgt gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc
    26521 atccaggtca tcagggaaac gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa
    26581 cccgtcgacc ccacgatcgg cccagaactc acagactttt ttcagctctg cgcgtaccgc
    26641 tggattctcc cagttgagat ccgcctgttc tggtgcaaag agatgcaaat agtactgttc
    26701 gctttccgca tgccagcgcc acgcactacc gccaaatttt gaacgccagt tgttcggtgg
    26761 cgtttctggt tcgccatcgc gccagatata aaactggcgg taagggcttt ctttgttcag
    26821 cgcctcgcga aaccaggcat gttgggtaga ggtatggtta aacaccatat cgagaatgat
    26881 acgaatcccg cgcgattttg cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta
    26941 ggtgggatca atcgccgtat agttcgctac atcgtaaccg ttatcgacct gcggagagac
    27001 ataaaagggg gttagccaga tggcatcaac gcccagttta tgcagatagt ccaggcgttg
    27061 gataacgcca cgtaaatcgc cggtgccgct acccgtggtg tcctgaaaac tctttggata
    27121 aatctggtag ataacgccgt tttgccacca gtggggaaga ttagtcatta cgttattcct
    27181 gcaaatgcga gggggcgcaa ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca
    27241 ggcggtattt ccgctgatag ataaacgagg tgagtacaat cgggatgatg atggcgatag
    27301 ccattgccag cgcaaacacc tgccagtagc tcggttgaat cgagagaatt cccggcaggc
    27361 cgcctacgcc gatgccattc gccataacgc cgttcaggcc gcatagcaat cctgccagac
    27421 cagaaccaat catcgcgcac agcatcggga agcgatattt caggttgatg ccgtacattg
    27481 caggctcagt gaccccaagc caggcggaga tcgccgcagg cacggagatc tcgcgttcat
    27541 tgtgcttgcg gctggaaatg ataatgccta tcacggcgga gccctgagcg atattcgaca
    27601 gcgcaatcag cggccacact ggcgtaccac ccatgctttg aatcatctgc aaatcaatag
    27661 caagcgtggt ctggtgtaca ccggtgatca ccagcggggc gtacaggaag ccaaacaatg
    27721 ccgcgccaat cggagcaaag ctgccggtca tcaggtgacg taccgcaaag gcaacgccat
    27781 cgccaatcat gcgaccaaac ggaccaatca gcgcatgggc gaggaacacc gcgaggatca
    27841 gcgaacagac gggcaccacc accagataga ggtaatccgg cacgatgcgt ttaaggcgag
    27901 tttcaataac gcccagcgcc agcccggcta acagtaccgg gatcacctgc gcctgatagc
    27961 ccactttggc gatgctgaac atgccaaagt cccacacttc cggcagctgc tgcccgagca
    28021 gataagcgtt catcagttgg ggggaaacca gtgtcacgcc aagcacgata ccaaggatcg
    28081 gcgtgccgcc catttttttc accgctgacc agcaaatccc gaccggcagg tagaagaaga
    28141 tcgcttcacc gatcaaccac agaaaatcgt agatcgtttg cagggaaggg tacatttgcg
    28201 ccagcgtttg accgttgctc atgggcaaat cgccgatcac attgcgaaaa ccgaggatca
    28261 aaccgccgct aatcaacgcg ggcagcaacg ggaagaagat ctctgcgaaa tgagagatca
    28321 actgctcatg ccatttcata ttctgccggg cggctttttt tacctgctct ttatcaacct
    28381 gcgcctgtcc ggttgacgct atcagtgctt gatagtaatc acccacgttg gtgccaatca
    28441 ccacctgaaa ttgcccggca ttggtgaaac agcctttcac catggggagt tgctcaattt
    28501 ctttcggtct ggcattggcc ggttggttga ggacaaagcg caggcgagta atacagtggc
    28561 tcaccgtcgc aatattgccg cgcccgccga ccagttcaat caaccgatcg atatccgttt
    28621 ggtttatttt gctcatcata aagccccatg gcagatgaca tttttggttg gctgcagaat
    28681 atattgcgcg gaaaaattta aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt
    28741 tatcgttcag gaaacgatca ggacaggttg gcggggatga tgatttgttg cggttcgctg
    28801 cgtccggtta cctgcgcgat caactggcaa gccgcctggc gtccagcttc ggcgtaacca
    28861 ggatctacgg tgacgatctc cggatgaagg aatttcatta acggcgtatt accgacgctc
    28921 gccagttgca aggtgtcgat gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc
    28981 gtgtcggttg cgcacagtaa ggcggtagtt tcaggcgtaa tcacttttgc tacgttctca
    29041 tagccttgct tcatagcaag ccctggcagg gcggcaacgg gatgcagttt atgcgctttg
    29101 cagaacgcca ggtaggcttc gtgacgtcgc ttaccggttg tcacgtcact gtgcggcacg
    29161 ccgagataac tgatattacg atgcccctgg tcatacagcc gttgcatcag gatcttgatt
    29221 gccccttcgt cgtcataaca gaccgaagca aagccttttg cgtcacgcgc cagcagaacc
    29281 agcgatgact gccagtgggc taacatttct tctgttatgc cagtaaaacc gaacagcact
    29341 acgccgtcga tattacgccg tttcagcacc cccaaatgtt cggcaactaa ttgcggggaa
    29401 aactgacttt ccatcatgat tgggtcgtaa ccttgttcat agaacgctgg cagcatggtt
    29461 tgaacggcga gattttctga caacgaatcc agacgggtaa caatgatggc gaccacttta
    29521 tcgctttgcc cacgcatagc gcgcgcagaa cgggaagggg aaaatccatg ctgattcatc
    29581 actgcttcaa cacgctcgcg ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg
    29641 gaaactgtag atttccccac gccgcttaag cgcgcgatgt ctttgatggt cagccgattt
    29701 tgcatcctgt tgtcctgtaa cgtgttgttt aattatttga gcctaacttt acccgtgcat
    29761 tcagcaatgg gtaaagtctg gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc
    29821 atagatgcta cgaatattat tggattctcc ttattatttg cggcgctttt ttcacttacc
    29881 ggaggttata tggaacctga tcccacgcct ctccctcgac ggagattaaa acttttccgg
    29941 taagcccgtc ttttcacggc gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg
    30001 ctcagcttta ttaccttcag gtaaggcttc gccacgcctg aagacatttc tgtactgttt
    30061 cagacagtgc ggagggactc cttatgttta aagaaatttt tacccggctc attcgccatt
    30121 taccttcccg tctggttcat cgtgatccat tgcctggcgc gcagcagaca gtgaatacgg
    30181 tggtcccgcc gtccttaagt gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat
    30241 tgtggaaaac gttcgacacc catccggaag ggttaaatca ggcggaagtg gaatctgccc
    30301 gcgaacaaca tggtgaaaat aaattacccg cacaacaacc gtcgccgtgg tgggtacatt
    30361 tatgggtctg ctatcgcaac ccctttaata ttttactcac cattctcggc gctatttctt
    30421 acgccacgga agatttattt gccgcgggcg ttatcgcgct aatggtcgct atttcaacgt
    30481 tgctgaactt tattcaggaa gcacgttcca ctaaagcggc agatgccctg aaagcgatgg
    30541 tcagcaatac cgcgacggtg ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg
    30601 agatcccgat cgaccagctg gtgcccggcg atattataaa actggcggcg ggagatatga
    30661 tcccggcaga tttacgtatc ttgcaggcgc gggatctgtt cgtcgctcag gcgtcgttaa
    30721 ccggtgagtc tctgcccgtg gaaaaagccg ctaccactcg ccagccggag cacagcaatc
    30781 cgctggagtg cgacacgctg tgttttatgg gcaccaccgt ggtgagcggc acggcacaag
    30841 caatggtgat tgctacaggt gccaatacct ggtttggtca actggcgggg cgtgttagtg
    30901 agcaggaaag cgagccgaat gcctttcagc aagggatcag ccgcgtcagt atgctgctga
    30961 ttcgctttat gctggtgatg gcaccggtgg tgctgttaat caatggctac accaaaggcg
    31021 actggtggga agcggcgctg tttgcgcttt cggtcgcggt aggcctaacg ccggaaatgt
    31081 tgccgatgat tgttacctcg acgctggcgc gcggcgcagt aaagctgtcg aaacagaaag
    31141 tgatcgtcaa acatctggat gctattcaga actttggcgc aatggatatt ctgtgcactg
    31201 ataaaaccgg caccctgaca caggataaaa ttgtgctgga gaatcatacc gatatctccg
    31261 gtaaaaccag cgaacgcgtg ctgcatagcg cgtggttgaa tagtcattat cagaccggac
    31321 ttaaaaacct gctcgataca gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc
    31381 tggccagtcg ttggcaaaaa atcgatgaga ttccgtttga tttcgagcgt cgccggatgt
    31441 cggtggtagt ggcagaaaat accgagcacc atcagctggt ttgcaaaggt gcattgcagg
    31501 aaatcctcaa tgtgtgttcg caggtgcgtc acaatggcga gattgtgccg ctcgatgaca
    31561 tcatgctgcg taagattaag cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg
    31621 ttgcggtggc gacgaaatac ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat
    31681 ccgacctgat cctcgaagga tatattgctt tccttgatcc gccaaaagag acaaccgctc
    31741 cggcactgaa ggcattaaaa gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg
    31801 agttagttgc agcgaaagtg tgccatgaag tggggctgga cgcgggagag gtggtcattg
    31861 gtagtgatat tgaaacgcta tctgacgacg aactggcaaa tcttgcacag cgcactacgc
    31921 tgtttgcccg cctgacgccg atgcataaag aacgcattgt gaccttactg aagcgcgaag
    31981 ggcatgtggt tggctttatg ggcgatggta ttaatgatgc gcccgcctta cgcgctgcgg
    32041 atatcggcat ttctgtggac ggcgcggtag atattgcccg tgaagcggct gatatcatcc
    32101 tgctggaaaa aagcctgatg gtgctggaag agggggttat tgagggacgt cgcactttcg
    32161 ccaacatgct gaaatacatc aaaatgacgg cgagttctaa cttcggtaat gtgttcagcg
    32221 tgctggtggc gagtgctttc ttgcccttcc tgccgatgtt gccgttacac ttgcttattc
    32281 agaacctgct gtacgatgtg tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc
    32341 aaattcaaaa gccgcagcgt tggaatccgg cggatctggg gcgctttatg atcttcttcg
    32401 gaccgatcag ctcgatcttc gatattctga cgttttgcct gatgtggtgg gtattccatg
    32461 ccaacacgcc ggaaacgcaa acgctgttcc agtcgggatg gtttgtggtg ggcttactgt
    32521 cgcaaacgct gattgtgcat atgatccgca cccgccgtgt gccgtttatt cagagctgtg
    32581 catcgtggcc gttaatgatc atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt
    32641 tttcaccgct ggccagttat ctgcaattac aggcgctgcc gttaagctat ttcccgtggc
    32701 tggttgcgat tctggcaggg tatatgacat taacccagtt ggtgaaaggg ttctatagcc
    32761 gtcgttacgg ctggcaataa agaataaact gggcacgata gccccgattc agattgcaga
    32821 caaagtaagc tttgttcatg ccggatgcgg cgtaaatgcc ttatccggcc aacaaattca
    32881 ataaattgca ggaatcacgt aggcctgata agcgtagcgc atcaggcgat tttgcgtttg
    32941 tcatcagact taatccgggc atgatagccc ggatttccat caagattagc gacgaacagc
    33001 gatcgcttcg atctcaatct tcacgtcttt cggcagacgg gcaacttcaa cgcaagaacg
    33061 tgccgggaag gtggcgttgt gttcggtgaa gaaggcttcg taagtggcgt ttacggttgc
    33121 gaagtcgttc agatctttta caaacacggt agttttaacg atgtcgccca ctttcagacc
    33181 agcggcttcg acgatcgctt ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc
    33241 tgccggtact tcgcccgttt tcggatttac cgggatctga ccggaagtga tgatcatatt
    33301 gcccagatca acgccctgta cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat
    33361 agttttgctc atgatttctc ctttattaca gcggtaaaaa gtctggctca ttatagggag
    33421 ccagactttt attaccaacc gcaattaatt ggccagcacc acattatggg aaaactcttt
    33481 ttcacagtat ttgcatttga gcgcgatatc attggcgcgt tttcgcacgg caaagctgga
    33541 agaaaccggt tcggcatggc tgatacagtt gctgttcggg cagaccagca cattgtcgat
    33601 gcgctccggc agacttgggc gcgatttacc caccacttca tagttgtcga tacggttaac
    33661 cgtggcttgc ggcgcataca atgccagttg atctacctgg tcttcactca aaaaggtatt
    33721 ttcgattttg atcagatctt tgcggcccat ctcgccagaa ggcaggttca gaccgatggt
    33781 gatgcgctga tccgtttcgg tcagcttgaa cagactcaac agcttaaaac cgatctgggc
    33841 ggggatatgg tcaattaccg tgccgcgttt aatagcttca acctgcaatt tattatcgtg
    33901 tgtcatctct atttcccctt acagtaccag atcgcgattc agaaccagtg ccagtaacgc
    33961 ctggcgagcg aaaatcccgt tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc
    34021 aacatccgtc gcaatctcat caacacgcgg cagcggatga agtactttca tattggcttt
    34081 ggcgttgtgg agatcgctgg cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga
    34141 cgggtccaga cgctcttttt gcacgcgggt catgtacagg atgtcaactt ccgccatcac
    34201 ttcttcaata gagctgtgca gactccatgc gatccctttt tcatcaagca tatccagaat
    34261 gtattgcggc attgccagcg cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt
    34321 cgctaacgcc tgagtcaggg agtgaacggt gcggccatat ttcaggtcac caaccattgc
    34381 gacgtggaga ttgtccagac gcccctgggt ttcctgaatg gtgaataagt ccagcaaggt
    34441 ttgcgtcgga tgttggttgg agccatcacc ggcattcagt accggtacat tgccggaaaa
    34501 ctcggtggcc aggcgcgccg caccttcctg cggatgacgc atcactatcg catcgacgta
    34561 agtgctgata accgaaatgg tatcggccag cgtttcgccc tttttaccca gtgatgtatt
    34621 ggcgctgtcg gagaagccca ccacgctggc acccaggcgg tgcatggaag tttcgaaaga
    34681 gaggcgggta cgggtagagg cttcgaagaa acagctggca atgactttgt gcttcaacag
    34741 ctctggttgc gggtttgctt tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc
    34801 gcgactaagg tcgtttatgg aaatgatatg tttctgatat agcggattag ccatctttta
    34861 tctcctgacg cctgggcaaa aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa
    34921 cgggaagaaa aacggcaggc cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac
    34981 acactgaacc atacatcctc ccggcaaatt gtccggcatt atactcatcg tcagaagcgg
    35041 tgcaagcatt tgatgcaata ttttgtcagc gcaaacggtt tatttgaatt ataagtcaaa
    35101 atatatgcat ttttatttac tgtgattctg gaggggaact gtcgttcggt atcaataatt
    35161 gcagacattt cctgcttttt tctttttcac tttcacgcaa tcagatatgc attttattcc
    35221 attcatccga cttatagggc ggagtgtgaa aacgaacggc taacactatt gcttacagct
    35281 cagggatgcg cgctatcact ttaatttcaa aatcaaagcc tgccagccat gtaacgccca
    35341 ccgccgtcca ggttggataa ggtggcgcgc taaatatttc atttttcacc gtcatgatgt
    35401 cttcaaattg tttttctgga tcggtatgaa agctcgtaac atcaatgata tcgtcaaaag
    35461 tgcatcccgc agctgccagg gtcgcatgca aattatcaaa tgccagtctg acttgttgct
    35521 gaaaatcggg ttctggtgtt ccgtcctctc gacttcctac ttgcccggaa acaaacagca
    35581 aatcgccgga acgaatagcc gcagaataac gatgttctgc atacagtgaa tgtcggccag
    35641 cagggaaaac agcggttctt tctaccattt ggttatcctc aagatttacg acatgaacag
    35701 aagatttata tttaccagga gccgctttta gcggacgacg tgagtaaaca aaacccagac
    35761 atcatgggaa atggctgggc ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca
    35821 atggtatgca tcgcgccggt aacaaaactg gcttctggcc ctgctaacca tgcgaccata
    35881 ccagcgacct cttccggttg cccatgtctt ttgatagcca taaaactatg caacatatcg
    35941 cgcattggcc cgttggcggg attagcgtcg gtatcaattg gccctggctg gacgacgttg
    36001 atggtgatcc cacgcggtcc aaaatcacgg gacagcccgc gcgccatgcc ttgtagggca
    36061 gatttactgg cggcataagc agccatgcct gcaacaggca tacgatcgcc attcacggag
    36121 ccgatgatta agatgcgccc accttcgggc atctgccggg cggcttcaac agaggcatgg
    36181 taaggagcat gaatattgat tttgaaaagg cgatcaatat cgtcggcatt taattccagg
    36241 gcatcgccaa agacgccaat acctgcattt accaccagga tatccaatgc gccgctctta
    36301 cgaacgacat caatgacagc gtctctgtca gcactatctg taaatactgc tgtcgctccc
    36361 gtctcttgtg ccaggcgttc agcggcatct ttcgaccccg cataggtgaa tcgtacattg
    36421 gccccatcgg tgacgaaacg acgtacgata gcggcaccga ttccacgact gccaccgagg
    36481 atgagaactg tcttacctgt aaaagcgccc ataaggactc cttgatttat tatgtaacat
    36541 gcattacaaa actgttttaa ctttctgtca acaagttttg taatggtcac taaaaaacaa
    36601 tctcgcgttc caggtcgtcc cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa
    36661 gtgctttttc accaaaaagg tttcgatgct gtcagtgttg ctgaagttac tgattatctt
    36721 ggtattaacc ccccgagcct ctacgcggct tttggcagta aggctgggtt atttagccgt
    36781 gtactcaatg aatatgtcgg tacggaagct attccgcttg tcgatattct tcgtgatgat
    36841 cgtccggtag gggagtgcct ggctgaggta ttaaaagaag cggcgcgcag atatagccaa
    36901 aacggcggct gcgctggctg tatggttctt gaaggcattc atagtcatga tccacaagcg
    36961 cgtgatattg ccgttcaata ttatcacgcc gcagaaacgg ccatttatga ctatatcgcc
    37021 aggctgcatc cacaaagcgc acaatgtgtg actgatttta tgagtaccgt gatgtcaggg
    37081 ctttctgcga aggcacgaga ggggcactcc ctcgaacaac tctgtgcaac tgctgcactg
    37141 gcgggggaag cgataaaaac tattctcaag gagtgatgct ggccttgatc cgaaaggcgg
    37201 gaacgcgcct gccgataagt tgtgataaga caataattca cgcgttaagg ctggctgaat
    37261 tgatcatctt ttcgtataac ggtggaaatg aaacattgtt ttaattaagg agtggaaaag
    37321 atgatcatcg gaaatattca taatcttcag ccgtggctac cccaggagtt acgccaggcg
    37381 attgagcata tcaaagcaca cgttacggca gaaacgccaa agggcaagca cgatatcgaa
    37441 ggtaatcgtc tgttttatct tatctcggaa gatatgaccg agccgtacga agctcgccgt
    37501 gcggagtacc atgcccgcta tctcgacatt cagattgtgt taaaaggtca ggaaggcatg
    37561 accttcagca cacaaccggc aggtacgccg gataccgact ggttagctga taaagacatc
    37621 gcatttttgc cggaaggcgt tgatgagaaa acagttatcc ttaatgaagg tgattttgtt
    37681 gtgttttatc cgggggaagt gcataaaccg ctgtgcgcag tgggtgcacc agcccaggtt
    37741 cgcaaagcag tagtgaagat gctgatggcg tgatgacttt tcgccgtaaa taactccagg
    37801 tttacggcga gtttgtgaaa agagcgtttt ttgatatttt tttgtgagta aaatttgtaa
    37861 tgcttagacg ttcttattaa ctcaaggagt tcgtcatgag caaaatatca gatttgaatt
    37921 attctcaaca cattacatta gccgacaatt ttaaacaaaa aagtgaagtt ttaaatacct
    37981 ggcgtgttgg aatgaataat tttgcccgta atgccggggg gcaggataac acaagaaata
    38041 tccttaatcc taagacattt ttggagtttt tggtaaaaat atttaccctg ggttatgtgg
    38101 attttagcaa acgctccaac gaagcgggaa gaaatatgat ggctcatatt gagtcctcat
    38161 cttatatcaa aaataatgat ggcagtgaga taatgaagtt tgttatgaat aatcctgaag
    38221 gggaacgagc ggattcaccc aaggtgatta tagaaatttc actttccact attactacta
    38281 tggggactcg tcaaggacat acagccatta tatttccaca acctgatggt tcgactaacc
    38341 gttatgaaag aaagtccttt gaaagaaaag atgagagttc attacacctg attactaaca
    38401 aggttctggc gtgttaccaa cgcgaagcta acaaggaaat agctcgtcta ttaaataatc
    38461 atcagaagtt aaataatcta cagaagttaa ataatctaca gaagttaaat aat
//
LOCUS       DAZGXS010000028        38011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGXS010000028 DAZGXS010000000
VERSION     DAZGXS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            221..2464
                     /gene="pdeF"
                     /locus_tag="ACHKG5_001604"
     CDS             221..2464
                     /gene="pdeF"
                     /locus_tag="ACHKG5_001604"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0867727.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLML
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2503..4044)
                     /gene="ppx"
                     /locus_tag="ACHKG5_001605"
     CDS             complement(2503..4044)
                     /gene="ppx"
                     /locus_tag="ACHKG5_001605"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0867728.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4049..6115)
                     /gene="ppk1"
                     /locus_tag="ACHKG5_001606"
     CDS             complement(4049..6115)
                     /gene="ppk1"
                     /locus_tag="ACHKG5_001606"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0867729.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6286..6924)
                     /gene="purN"
                     /locus_tag="ACHKG5_001607"
     CDS             complement(6286..6924)
                     /gene="purN"
                     /locus_tag="ACHKG5_001607"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0867730.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6924..7961)
                     /gene="purM"
                     /locus_tag="ACHKG5_001608"
     CDS             complement(6924..7961)
                     /gene="purM"
                     /locus_tag="ACHKG5_001608"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0867731.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8286..8912
                     /gene="upp"
                     /locus_tag="ACHKG5_001609"
     CDS             8286..8912
                     /gene="upp"
                     /locus_tag="ACHKG5_001609"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0867732.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8998..10287
                     /gene="uraA"
                     /locus_tag="ACHKG5_001610"
     CDS             8998..10287
                     /gene="uraA"
                     /locus_tag="ACHKG5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0867733.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10382..11083
                     /gene="hda"
                     /locus_tag="ACHKG5_001611"
     CDS             10382..11083
                     /gene="hda"
                     /locus_tag="ACHKG5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0867734.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11221..11580)
                     /gene="arsC"
                     /locus_tag="ACHKG5_001612"
     CDS             complement(11221..11580)
                     /gene="arsC"
                     /locus_tag="ACHKG5_001612"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0867735.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11601..13064)
                     /gene="bepA"
                     /locus_tag="ACHKG5_001613"
     CDS             complement(11601..13064)
                     /gene="bepA"
                     /locus_tag="ACHKG5_001613"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0867736.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13277..14338
                     /gene="yfgO"
                     /locus_tag="ACHKG5_001614"
     CDS             13277..14338
                     /gene="yfgO"
                     /locus_tag="ACHKG5_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0867737.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14376..15224)
                     /gene="focB"
                     /locus_tag="ACHKG5_001615"
     CDS             complement(14376..15224)
                     /gene="focB"
                     /locus_tag="ACHKG5_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP0867738.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15246..17258)
                     /gene="hyfR"
                     /locus_tag="ACHKG5_001616"
     CDS             complement(15246..17258)
                     /gene="hyfR"
                     /locus_tag="ACHKG5_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP0867739.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17288..17701)
                     /gene="hyfJ"
                     /locus_tag="ACHKG5_001617"
     CDS             complement(17288..17701)
                     /gene="hyfJ"
                     /locus_tag="ACHKG5_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP0867740.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17694..18452)
                     /gene="hyfI"
                     /locus_tag="ACHKG5_001618"
     CDS             complement(17694..18452)
                     /gene="hyfI"
                     /locus_tag="ACHKG5_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP0867741.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18449..18994)
                     /gene="hyfH"
                     /locus_tag="ACHKG5_001619"
     CDS             complement(18449..18994)
                     /gene="hyfH"
                     /locus_tag="ACHKG5_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP0867742.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(19004..20719)
                     /gene="hyfG"
                     /locus_tag="ACHKG5_001620"
     CDS             complement(19004..20719)
                     /gene="hyfG"
                     /locus_tag="ACHKG5_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP0867743.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20709..22289)
                     /gene="hyfF"
                     /locus_tag="ACHKG5_001621"
     CDS             complement(20709..22289)
                     /gene="hyfF"
                     /locus_tag="ACHKG5_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP0867744.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVAVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22294..22944)
                     /gene="hyfE"
                     /locus_tag="ACHKG5_001622"
     CDS             complement(22294..22944)
                     /gene="hyfE"
                     /locus_tag="ACHKG5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP0867745.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22956..24395)
                     /gene="hyfD"
                     /locus_tag="ACHKG5_001623"
     CDS             complement(22956..24395)
                     /gene="hyfD"
                     /locus_tag="ACHKG5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP0867746.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24412..25359)
                     /gene="hyfC"
                     /locus_tag="ACHKG5_001624"
     CDS             complement(24412..25359)
                     /gene="hyfC"
                     /locus_tag="ACHKG5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP0867747.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25370..27388)
                     /gene="hyfB"
                     /locus_tag="ACHKG5_001625"
     CDS             complement(25370..27388)
                     /gene="hyfB"
                     /locus_tag="ACHKG5_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP0867748.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27388..28005)
                     /gene="hyfA"
                     /locus_tag="ACHKG5_001626"
     CDS             complement(27388..28005)
                     /gene="hyfA"
                     /locus_tag="ACHKG5_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP0867749.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28258..28728)
                     /gene="bcp"
                     /locus_tag="ACHKG5_001627"
     CDS             complement(28258..28728)
                     /gene="bcp"
                     /locus_tag="ACHKG5_001627"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0867750.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28728..29300)
                     /gene="gcvR"
                     /locus_tag="ACHKG5_001628"
     CDS             complement(28728..29300)
                     /gene="gcvR"
                     /locus_tag="ACHKG5_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0867751.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29446..30324
                     /gene="dapA"
                     /locus_tag="ACHKG5_001629"
     CDS             29446..30324
                     /gene="dapA"
                     /locus_tag="ACHKG5_001629"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0867752.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30341..31375
                     /gene="bamC"
                     /locus_tag="ACHKG5_001630"
     CDS             30341..31375
                     /gene="bamC"
                     /locus_tag="ACHKG5_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0867753.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31588..32301
                     /gene="purC"
                     /locus_tag="ACHKG5_001631"
     CDS             31588..32301
                     /gene="purC"
                     /locus_tag="ACHKG5_001631"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0867754.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32469..33332
                     /gene="ypfJ"
                     /locus_tag="ACHKG5_001632"
     CDS             32469..33332
                     /gene="ypfJ"
                     /locus_tag="ACHKG5_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0867755.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33347..35362
                     /gene="tmcA"
                     /locus_tag="ACHKG5_001633"
     CDS             33347..35362
                     /gene="tmcA"
                     /locus_tag="ACHKG5_001633"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0867756.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35436..36134
                     /gene="ypfH"
                     /locus_tag="ACHKG5_001634"
     CDS             35436..36134
                     /gene="ypfH"
                     /locus_tag="ACHKG5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0867757.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36215..36415)
                     /gene="ypfN"
                     /locus_tag="ACHKG5_001635"
     CDS             complement(36215..36415)
                     /gene="ypfN"
                     /locus_tag="ACHKG5_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0867758.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36443..37570)
                     /gene="dapE"
                     /locus_tag="ACHKG5_001636"
     CDS             complement(36443..37570)
                     /gene="dapE"
                     /locus_tag="ACHKG5_001636"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0867759.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37574..37930)
                     /gene="yffB"
                     /locus_tag="ACHKG5_001637"
     CDS             complement(37574..37930)
                     /gene="yffB"
                     /locus_tag="ACHKG5_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0867760.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWHKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         9251 a         9837 c        10003 g         8920 t
ORIGIN      
        1 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
       61 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
      121 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
      181 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta
      241 tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc
      301 cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaggttt ttctctttta
      361 tctgcctctg gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg
      421 gatcgcctta gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc
      481 attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac
      541 tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg
      601 gcagattgtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg catttgtagg
      661 attactggcg agcagagaaa atctggttgg cgtcatgcct tttaatctcg ggaccttaat
      721 caattatcag gccttactgg tgggcaatct gattggtgtc ccgctgtgct acttcatcat
      781 tcgggtagtg cgaaatccgt tttatttacg tagttattat tcgcaattaa aacagcaggt
      841 tgatgtcaaa gtcaccaaaa aagagttcgc actctggctg ctggcattag gtgctttact
      901 gttgctgtta tgcatgccgt taaatgaaaa aagcacaatt tttagcacca attatacctt
      961 gtcattattg ctgcccctga tgatgtgggg agcgatgcgc tatggttata agctgatttc
     1021 gctgctctgg gcggtcgtgt tgatgctcag catccacagc tatcaaaatt acattcccat
     1081 ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt
     1141 tattgtcaat tatatggctg tactggcaac ccgtcagaga acggtagtca gacgcattca
     1201 gcgccttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc
     1261 gttgcgtgat gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct
     1321 ggttaaaaac tatggcatca tgctgcggat tcaatacaag caaaaacttt ctcactggct
     1381 gtcacccttg ctggaaccag gtgaagatgt ttatcagctt tcgggtaacg atctcgcgct
     1441 gcgactgaat acagaatcgc accaggagcg cattaccgca ctggatagcc atctcaagca
     1501 atttcgtttc ttttgggatg gcatgccgat gcaaccgcag attggcgtca gttactgcta
     1561 tgtgcgctcg ccagtgaatc atatctacct gctgctggga gagctaaata cggtcgccga
     1621 actttccatc gtgaccaacg ccccggaaaa tatgcagcgt cgcggggcaa tgtatttgca
     1681 acgcgaattg aaagataaag tcgcgatgat gaatcgacta cagcaggcgc tggaacacaa
     1741 ccattttttc ctgatggccc agccgattac cggtatgcgt ggtgatgttt accatgaaat
     1801 tcttctgcgc atgaaaggtg agaatgatga actaatcggc cccgatagct tcttgccggt
     1861 cgcgcacgaa tttggtttat cgtcgagtat cgacatgtgg gtcattgagc atacgctgca
     1921 atttatggct gaaaacagag cgaagatgcc cgctcaccgt tttgctatta atctgtctcc
     1981 aacctcggta tgtcaggctc gttttcctgt tgaagtcagt cagttgctgg ataaatatca
     2041 gattgaagcg tggcaactta tttttgaagt caccgaaagt aatgctctga ccaatgttaa
     2101 gcaggcgcaa atcaccttgc agcatcttca ggaattaggc tgccagattg cgattgatga
     2161 tttcggcacc ggctacgcca gctatgcgcg gcttaaaaat gtgaatgccg atctgcttaa
     2221 aattgacggc agttttatcc gcaatattgt gtcaaatagt ctggattatc agatagtggc
     2281 atcgatttgc cacctggcgc gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa
     2341 cgaagagatc cgcgaggcgg tgctctcttt ggggatcgat tatatgcagg gttatcttat
     2401 tggtaagccg caaccgttaa ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc
     2461 ctgaataatg cgggccgaca tttctcgtcg gcccgcaaag cattaagctg cgatttcagg
     2521 tgtactttct tcttcaattt tcaaccgcca gccagccacg ccttcccagt attcttgctc
     2581 cttttccaga tcaagcagta ccagcgcatt ctgactaaac cagtcatgcg ggaaacgcag
     2641 tgtccagtga ctgtcatcag taatcagcgt caatgttggc ggtgtggtgg ttgcctgacg
     2701 ctgattgttg agtaatacgc caaggcgcaa tagctgaatc agtggcagga actgtttctt
     2761 cttaaacaag gtaaagcgcg gcagatcgtc gagtttaatc gctttacggt gatagcgcac
     2821 cagtgtcgcc atcatcagct gctgttcctg attaaaaccc ggcaagtcgc tgttttgtag
     2881 aatataagcg gagtgacgat gcaaaccgct gtggttgata ttcaacccga cctcatgcag
     2941 catggcggcc catcgcagta gcgcctccag ttgcggatgc gccagcttcg gttgctgttc
     3001 ccgccactgt tcgtacattt gcatagtggt atccagcacc cgtcgggcct gttcgctgtc
     3061 gatgtgatac tggttggcga ggctgctggc ggtgcgacta cgcacatcct gatgacggaa
     3121 acgtccttcc atttcataca gtacgccttc gcgtaacgcc ccgtcagaaa ggcgcagttc
     3181 acggatggct aaagcatcaa acacaccgca taaaatcgct agtccaggaa cgaagactgt
     3241 tttccgctct tcggaaagac ctggtaaact cagcgatgcg aaattacggt gccgtaaaac
     3301 ttcttttacc agtttttcca gacgttccgg ggtaattatc ccgtctttct cgcccatttc
     3361 cattaacact tcatgggcgg cttttatggt accggaagcg cccattgcca cgttccagcc
     3421 ctgaatacga aattgccagg ttaaagtttc cagtttttgt gctgccgcca tgcgagcgcg
     3481 ctgaaaattc tctttattga tgaccccgcc agggaaataa agctgggcaa agctgacaca
     3541 ccccatccgg cggctttcaa cgagaatcgg ttcgaaattt tcaccaatca ccagttctgt
     3601 agaaccgccg ccaatatcaa taaccagttt gcgacctttt tccggttggg tatgttccac
     3661 gcccataaaa atcagacggg cttcttcatt accggaaata atttcaatcg ggtagggaat
     3721 gaccttttcc gcacgtttca gaaagtcagt ggcgttcagc gcctgacgca gcgtatgggt
     3781 accaactata cagacgctgg caggagaaaa cccttgtagc cgttcggcaa acagcgacag
     3841 acagttcaaa ccgcgcgtca ttgcctcttc actcaacata ttatctggcc ccaggccgtc
     3901 cgccagatgc acccgctgtt tcaggcggcc aataatctgc atggcaccat ctaccacacg
     3961 ggctatgacc atgtgaaaac tgtttgaacc aagatcgacc gcagcaaact cctgcggacg
     4021 aggggattta tcgtgtattg gcatagggtt attcaggttg ttcgagtgat ttgatgtagt
     4081 cataaatcgc caactgcgcc cgtactttgc ggcgattgcc gcggggaacg tagcgattac
     4141 tgagttcttt atcgatataa cgtgctttga ccgtatcgct gaacaatatg tcgatgatgt
     4201 ccagcacccg ctgcttcagg cgcggatcga gcagcggcgt cgccacttca atacgataat
     4261 caatattgcg cgtcatccag tcggcggaag aaaggtagac ctttttatcg ccgccatttt
     4321 caaaaatata aacccggtca tgttcaaggt aacggtcaac aatactgatg gcacgaatgt
     4381 tgtcgctaat gccttccaga ttggggatca gcgaacacat tccgcgaacc agcagattaa
     4441 ccggtacgcc ggagctggag gccgcataca gacgatcaac caggccttta tcgacaaggt
     4501 tatttagctt cagggtgata ccactgggca gcccttgctg cgcgttggcg atctcgcggt
     4561 ccaccatttc atacaacagg cggcgggagt tttgcggcga caccattaaa taatcaaatg
     4621 tcaccggacg gtatgggttt tcaataaagt taaatacccg ccgtacttcg ttggtgatgc
     4681 gcgcatcggc ggtcagcaac gaatagtcag tataaagacg cgcggttttt tcgttaaagt
     4741 tcccggtccc gatgtgtgca taacgcacca cttcaccgtt ttctttacgt gaaatcagaa
     4801 acagtttggc gtgaattttc agccccggcg cagagaagat aacgtgcacg cctgcttcgg
     4861 tcaggcgctt cgcccagtga atgttggctt cttcgtcaaa acgtgcctgt agctcaacca
     4921 ccacggtgac tttcttaccg ttgtgtgcgg cgtggatcat cgagtcgatg atgcgtgaat
     4981 cttttgccac acggtaaatg ttgattttaa tcgccagcac gctcggatcg aacgaggcct
     5041 gacgcagcag ttccagcaca tgctcaaagg tgtgataagg ataatagagc aacacatcgc
     5101 gttcgcgaat ggcatcaaaa ccattgcgga actgggcttt atcaaaccaa atatggcgta
     5161 aacgcggcag tggtttgttc accagattgg ctttgccgac attggggaaa ttaataaagt
     5221 ctttaaaatt atgataacga ccgccgggga cgatggagtc gtagcgggaa atagtcagtt
     5281 tttcgcgtaa cacttcaacc agcgcattgg gcatatcgcg ctgataaaca aaacgcaccg
     5341 gctcagcagt taaacgctgc ttgagactgg aagacatcaa ctccatcagg ctggcttcca
     5401 tctcatgcac taaatcgtat tcggcatcgc gggtcatctt cattgaatag gcattcagcg
     5461 cgtcataatc aaagaagcct ttgaaaatat catcaaggca gtaacgcaga atgttatcca
     5521 gaagaatcat tggcttgcgt cgacgcggcg cttctggcgg taaattcaca aagcgcggca
     5581 ctttatctga tgggatctcc agaagcgcgt aacggatggt atcgccacgg ataatttcca
     5641 ccgccagata ggtgtaatca tctttcagga actgcactaa gtcagtgtca ggattgatta
     5701 aaatcggcgt aatgtgctga cgcagatact gcttaaaata atgacgcagc cagttttgtt
     5761 gattgacgga gagctggcgt tcattaatca ggaagatctg gttgcgcgcc atctccagca
     5821 atagctcgtt gtagaggccg tcgaattcct gatcggcttt cagcacccgg gactgaattt
     5881 tgcccagtaa atggcgggaa tgagagttgg agccttgttc ttcgctaata atgatgcgtc
     5941 gcttcagttc agcgaagcgg actttataga actcatcaag gttattggaa tagatcccca
     6001 ggaaacgcat cctttcaatc agcgggttag atttgtccgc cgcttcctga agcacgcgtt
     6061 cattgaacga taaccaactg agctcttttt cgatgtatag cttttcctga cccattacca
     6121 cttttactcc gttttattca cgggacactg cctggatatt atggcgagca atattggcag
     6181 atgtccaact atgccagaaa agggggaaaa aaactgctgg agcttgcgat ttacaggtga
     6241 attgaaaaac gccagcggca gagctggcgc tttaactacg gggcattact cgtcggcagc
     6301 gtagccctgc ggcggcagac gttgaccatc cagccacgcg gcgttttcgt gcattttcag
     6361 acgaccatcg gcaaaccagc taatcaccag tggataaatg gcgtgttcct gggtttgcac
     6421 gcgggcggtg acgtcatcct ccgtatcacc agcaaatacc gggactttcg cctgtaaaat
     6481 aaccgggcca ccgtccagtt catcggtgac gaaatgcacc gatgtaccgt gctcttcatc
     6541 gccattttcc agcgcctgac gatgggtatg taatccggga tatttcggca gcagagaagg
     6601 gtgaatgttc agcaaacgcc cggcatagtg ggagacaaat gccgggctga gaatgcgcat
     6661 aaaaccagcc agcacgacca catcgggtgc gtacatgtcg atttcatgaa tcaactcccg
     6721 gtcataggct tcacgactgt caaacgcgct ggcgatgagc gtatgcgttg caatacccgc
     6781 ctggcgggcg cgttcaaggc cgaacgcgtc ggccttattg ctgaaaactg cccgtacggt
     6841 gcctttaatt ttgttggttt tacaggcgtc aataattgcc tgcaaattac ttccgttgcc
     6901 ggaaataagc accacaatat tcattattcg ataaccacgc gttgttcgga atcagaggct
     6961 ttgatgatac cgattttcca cgcgttttca ccgttggcat tgagcagggc gagggctttg
     7021 tccacttccg gagcaggcag ggcaataatc atcccgacgc cgcagttgaa ggtgcgatac
     7081 atttcatggc gctcaacgtt accagccgtt tgcagccagt tgaacacttc cggccactgc
     7141 caggaggatt catcaatcac tgcctgggta ttatctggca atacgcgcgg aatgttttcc
     7201 caaaagccgc cgccggtcag gtgcgcaatg gcattaacat cgaccttttc aatcaactcc
     7261 agcactgact tcacgtaaat gcgggtcggt gccagcagat gatcggctaa tggcttacca
     7321 tcaagttcgg tggtttgcgg atcacaaccg ctgacttcaa gaattttgcg caccagcgaa
     7381 tagccgttcg agtgcggacc gctggaaccg agtgcaatca gcacatcgcc gtcgctgact
     7441 ttagagccgt cgatgatttc tgatttttct accacgccca cgcagaaacc cgcgacatcg
     7501 taatcctcac cgtgatacat ccccggcatt tctgccgttt cgccacccac cagtgaacag
     7561 cccgattgca gacaaccttc cgcaatgccg ctgatcaccg ctgaagcggt atcaacatcc
     7621 agttttccgg ttgcgtaata gtcgaggaaa aacagcggtt ctgcaccttg caccaccagg
     7681 tcattaacgc acatggcgac cagatcaata ccaatggtgt cgtgacgttt taagtccatt
     7741 gccagacgca gcttggtacc tacgccgtca gtgccggaaa ccagcacggg ttcacgatat
     7801 ttttgcggca atgcacacag cgcaccgaag ccgcccagac cgcccatcac ttccggacga
     7861 cgcgttttct tcactacgcc tttgattctt ccaaccagag cattacccgc gtcaatatca
     7921 acaccggcat ctttgtagct aagagaggtt ttatcggtca ctgcttgggt ccccacgcgt
     7981 tacttgcggt agaaaaataa aattcggcgc aattctaaca gggaaagcaa acgtttgcga
     8041 gactgcttta cacaaccttt ttgcacgtct tttccccagg cgcgcggcga aagaagactt
     8101 gtgccagggc aaaggttagt tttcggatgg aataatcttc tttcataacc atttgaatat
     8161 aaaataactt tatctcaaac cgttatcatt ttgactaaag tcaacgaaaa taatattgcc
     8221 gccttgaaga aaggaggtat aatccgtcga ttttttttgt ggctgcccct caaaggagaa
     8281 agagtatgaa gatcgtggaa gtcaaacacc cactcgtcaa acacaagctg ggactgatgc
     8341 gtgagcaaga tatcagcacc aagcgctttc gcgaactcgc ttccgaagtg ggtagcctgc
     8401 tgacttacga agcgaccgcc gacctcgaaa cggaaaaagt aactatcgag ggctggaacg
     8461 gcccggtaga aatcgaccag atcaaaggta agaaaattac cgttgtgcca attctgcgtg
     8521 cgggtctggg gatgatggac ggtgtgctgg aaaacgttcc gagcgcgcgc atcagcgttg
     8581 tcggtatgta ccgtaatgaa gaaacgctgg agccggtacc gtacttccag aaactggttt
     8641 ctaacatcga tgagcgtatg gcgctgatcg ttgacccaat gctggcaacc ggtggttccg
     8701 ttattgcgac catcgacctg ctgaaaaaag cgggctgcag cagcatcaaa gttctggtgc
     8761 tggtagctgc gccagaaggt atcgctgcgc tggaaaaagc gcacccggac gtcgaactgt
     8821 ataccgcatc gattgatcag ggactgaacg agcacggata cattattccg ggcctcggcg
     8881 atgccggtga caaaatcttt ggtacgaaat aaagaataaa aataattaaa gccgacttta
     8941 agagtcggct tttttttgag taaagcgcct ataacacata atacagagga taatactatg
     9001 acgcgccgtg ctatcggggt gagtgaaaga ccgccacttt tacagacaat cccgcttagt
     9061 ttgcaacatt tgttcgccat gtttggtgca accgtcctgg tgcccgtctt atttcatatt
     9121 aacccggcga ctgtactgtt atttaacggt attggaacgc tgctgtatct cttcatctgt
     9181 aaagggaaaa ttccggctta tcttggttcc agctttgcct ttatttcacc ggtattgtta
     9241 ctgttgccgt tagggtatga agtcgcgctg ggcggcttta ttatgtgcgg cgtgctgttc
     9301 tgcctggttt cttttatcgt gaagaaagcg gggaccggct ggctggacgt gctgtttcca
     9361 cctgcggcaa tgggcgcaat cgttgccgtc atcggtctgg agctggcggg cgtagctgcc
     9421 ggtatggcgg gtttactccc ggctgaaggg caaacgccag actccaaaac catcatcatc
     9481 tctattacca ccctggcggt cacggtttta ggttccgtgc tgttccgtgg tttcctggca
     9541 attatcccga ttttaattgg cgtgctggtg gggtacgcgc tctctttcgc aatgggaatt
     9601 gtcgatacca cgccgattat taatgctcac tggtttgcgc tgccaaccct ctatacgccg
     9661 cgcttcgagt ggtttgccat tctgactatt ctgccagcgg cgttagtggt tattgccgaa
     9721 cacgtagggc acctggtagt aacggctaat atcgtcaaaa aagatctgct gcgcgatcca
     9781 ggtctgcacc gttcgatgtt tgctaacggc ttgtcgaccg tgatctccgg cttctttggc
     9841 tccacgccaa acaccaccta cggggaaaac attggcgtga tggcgatcac ccgtgtttac
     9901 agtacctggg ttatcggcgg ggcggcgatt ttcgctatcc tgctttcctg cgtcggtaaa
     9961 ctggctgccg ctatccagat gatcccattg ccggtgatgg gcggcgtttc gctgctgctt
    10021 tatggtgtca tcggtgcttc cggtattcgt gttttgatcg aatcgaaagt ggattacaac
    10081 aaagcacaga acctgatcct gacttccgtg attttgatca tcggcgtcag tggcgcgaag
    10141 gtaaacattg gcgcggcgga gctgaaaggt atggcgctgg cgaccatcgt cggtatcggc
    10201 ttaagtttga ttttcaaact gattagtgtg ctgcgccctg aagaagtggt gctggacgca
    10261 gaagacgcgg atataacaga caaataatgt accgaccggg cagtgttgct gcccggttca
    10321 aacatcatgg gattctgtgg taaacttctc gcgattttgt gaaatcctgg ttgaggtatc
    10381 tctgaacaca ccggcacagc tctctttgcc actttatctt cctgacgacg aaacctttgc
    10441 aagtttctgg ccgggggata actcctcttt actggccgcg ctgcaaaacg tgctgcgtca
    10501 ggaacatagc ggttacatct atctctgggc acgcgaaggc gcggggcgca gccatctgct
    10561 gcacgcggct tgcgcggaat tgtcgcagcg tggcgatgcg gtgggctatg tcccgctgga
    10621 taaacgcacc tggtttgttc cggaagtgct cgacggtatg gagcatttgt cgctggtctg
    10681 tatcgacaac attgagtgta ttgcaggcga tgagttgtgg gagatggcga ttttcgatct
    10741 ctacaatcga attctggaat cgggcaaaac acggttgttg atcaccggcg atcgtccacc
    10801 gcggcagttg aatctgggat taccggatct cgcgtcgcga ctcgactggg ggcagatcta
    10861 caaattgcag ccactttctg atgaagataa gttgcaggcg ctacagttac gcgcgcgttt
    10921 gcgtggtttt gaactgccgg aagatgtggg gcgtttcttg ctgaagcggc tcgacagaga
    10981 aatgcgcacg ctatttatga cgttggatca gttggatcgt gcgtcgatta ccgcgcaacg
    11041 taagctgacc attccgtttg tgaaagaaat tctgaagttg tagataaggt gtttattgtc
    11101 ggatgcggcg cgaacgcctt atccgaacta cgcgaagtta atccatagaa ttcgtaggcc
    11161 ggataagacg tggcaagcgt cgcatccggc aatgtgcttc aactagcgat ggagcacatc
    11221 ttaacccacg atttccagta cctgctccgg tggacgacca atccgcgctt tgccattcgc
    11281 gaccacaatc gggcgctcca tcagcttcgg attatccacc attgcctgaa tcagcgcctc
    11341 ttcgctaagc gaactgtcag ccaggttcag ttctttataa agatcctctt tctggcgcat
    11401 caattcacgg gcgctattca tccccagcat tttcagcaaa tcgcgcagcg ttgccgcatc
    11461 ggcgggtgtc tcaaggtaga gcaccacttc tggctccacg ccgttttctt tcagcaggtt
    11521 cagcgtttcc cggctctttg aacagcgtgg gttatggtag atttttacct gtttggtcat
    11581 tgttcttcct ttaatgcgaa ttacatcttg gtataaggct taaagcgttc ctgcagctgg
    11641 cgcaactggt cgatgcgcgc atcgtaacgc gcttgttgca ggctgcctaa tttcacctgc
    11701 gaactggcgc tactcaacag cgaaatggcc tgatcgagtc gtccggcgag cgcataacct
    11761 tctgctcgcg cagccagctc ctgatcgcgg ttatttagcg cggcttccgc ctgtgccagc
    11821 aaatcccagc cgttgctgtc atctttatta ttaaaggtgt agcgattaag aatattggcc
    11881 gcttcttgtg gttgaccgcc ttgtagataa gcgttcgcca ggttgagctg caacacagga
    11941 ttggtgcgca aatcgcgggc atttttcaga cgattgatcg cctcattggc tttgttttgc
    12001 ccaagatcga tatcagtagc cagatcgaga taccatgcgt tgccaggttc tgccgccagt
    12061 aacggttgca gcgtttttcg cgcctcgtcg tatttattgg cttccatcgc ctgtaaagca
    12121 cgaccatatt gcgccgctcg ttgctgacga acgtttcctt tcgcccattc atccagcaaa
    12181 tcactggtga gctggttacg tccggaatta tacatcccca gtgtgcgcgc tttcgccaga
    12241 tagaaatctt ccgacgactg caccaccatc gggcgcatct gattagcacg gttgcgggca
    12301 tctgccagac gactttccgg caacgggtga gtcagtaaaa tttccggcgg gcgcgaggag
    12361 taacgcgcct gatcgagtaa tttttcgagg aaggttggca tcgcctgcgg atcgaatccc
    12421 gagcgttgca gcacctgaat accaatgcgg tccgcttcct gttcattttg ctgggtgaaa
    12481 ctgatcatcc cctgacgcgt tcccgccagt gtaccggtca gcgccgccat ccccgcctgc
    12541 ggactggcca tcgccagtaa aatagaacct aacgcgccga cccaggtcag cggcgcgctg
    12601 cgctgctgat cttccatcgc tcgcgccagg tgacgttggg tgacgtggga gatttcgtgc
    12661 gccataactg aagccagttg actttcgtta tcggaataac ggaacagggc agagtgcagc
    12721 accacgttgc cgccaaagaa agcaaaggcg ttaatttcgt cgttgttgat cagaaagaaa
    12781 tgaaacggtg tcttaaccga attggcatgc gaaaccagac gcatccccag cgaattaata
    12841 tattgcgtta acagcgggtc attaattaac ggcgcgctgc cgcgtagctg gcggacataa
    12901 tagtcgccca tctgcatttc ctgaccaatg gaaagcgtgc ttcctgcgga ggttcccata
    12961 tccggcaagg tgtctgcgct gtcggcaaat gccggggcta cctgaccaat ggtcatagca
    13021 gcaatgaggg ttgcaaccag gttttttttc aactgcctga acataacctc tatcctgtat
    13081 ttctgaagat agtcatttga ccgatactgt gaaatatcgt tcccaccgcg ccgcctttga
    13141 gtgtaacggc gaagattagc ccactcaaat gacttttcag tttcctgaat ccgtcataaa
    13201 aagcgaagtc ttttttgtga catttcgata caattgcgga tcgcaatccc gctcttgagg
    13261 ttcagggaag gtctttatgc tcgaaatgtt gatgcaatgg tatcgccgcc gttttagcga
    13321 cccggaagcg attgccttgc tggttatttt agttgccgga tttggcatta tctttttctt
    13381 tagtggcctg cttgctccgt tgctggtggc tattgtgctg gcctatttgc tggaatggcc
    13441 aaccgtgcgc ctgcaatcta ttggctgctc ccgccgctgg gcgacgtcga ttgtattggt
    13501 ggttttcgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg cctggcagca
    13561 gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg actttgccgc
    13621 cacgttgccg cgccgctatc cggcgttaat ggatgcgggc attattgatg caatggccga
    13681 aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt ccctcgcctc
    13741 gctggtcggt ttgctgacca ttgccgtcta tctggtgctg gtaccattga tggtcttctt
    13801 cctgctgaaa gacaaagagc agatgctgaa tgccgttcgc cgggtgctgc cgcgtaaccg
    13861 tggactggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact atatccgcgg
    13921 caaagtgctg gagatgatcg tagtagggat cgccacctgg ctgggattct tactcttcgg
    13981 gctgaactat tcgctgctgc tggcggtgct ggtcggcttc tcggtactta ttccgtacat
    14041 tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt ttggtgcagg
    14101 cactgaattc tggagctgtt tcgcggtgta tctgatcatt caggcgcttg acggcaacct
    14161 gttagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga ttattttatc
    14221 ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca ttccattggc
    14281 gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatagcgc aagaataatc
    14341 ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg taaataaata
    14401 gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag catcaccggg
    14461 agcagattgg cggtaataaa atggctgacc gtcagtgcag gaaaattgtc tgcgctactg
    14521 tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc gaacggaatc
    14581 acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt gatgggcaaa
    14641 atcatggcga cgattttgtc gcataacgaa cgcccgcaat aactcatcca cagcgccagg
    14701 caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt atgatgcatt
    14761 ttgccctcgg cgcagtgtaa aaccgcgact ccccacatcg cgttttcact catcaccagc
    14821 ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc gcaggccact
    14881 agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt tgccatcacc
    14941 gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat aaagccgagt
    15001 gtaaagcaaa ggccgcccac cagatgggtt aatgcctgtg aagacggggc atcggcgata
    15061 acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat cgccattaaa
    15121 aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc gattgccgct
    15181 tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac tcctgcaaaa
    15241 gcagattaca acacctcgcg aaccgagatc cccagacgct gcattcgtga cagcagcgtg
    15301 gtgcgcttca tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat gccattggtt
    15361 tctcgcaata cctgaacaat gcgctgacgt tcttcttcgt cattctctgg cgtcgtcggg
    15421 tgcagcaact gcgccattga actgcgaagc gctgaatctt catttagcgt cggcagtaaa
    15481 cggctttgtc ggacattaag atgtaaattc agactgttac cgcgagtcag cagtaccgct
    15541 cgctcaatca cgttttccag ctcgcgcacg ttgcccggcc aatcccacga catcaactgg
    15601 cgtagtgcct cggtcgggat ggcgtcaatt gcgcgattca tatggcgcgc cattttttgc
    15661 gtgaaatgtt ttgctaaaag agggatatct tccggacggt ctcgcagcgg cggcaattcc
    15721 agtgggaaga cattcaggcg ataaaagaga tcgctgcgaa actggcgatc ttcaaccatt
    15781 tgccacaaat cacggttggt ggcggcaatg actctgacat tcaccgggat cgttctactc
    15841 ccgccgagac gctcaatttc ccgctcctgc aatacgcgca gcaatttagg ctgaagttct
    15901 aacggcagat cgccaatttc atcgagaaac aacgtaccgc catcggcaat ttcaaaacga
    15961 ccacgatggg tattaatcgc accagtaaag gcccctttgt catgaccgaa taactcactt
    16021 tccagaagac tggcggggat ggcagcgcag ttgattttga ccagcggctt gtcgcgtcgc
    16081 gggctaagtt gatggatcgc tctggcgatc acctctttgc cggttccggt ttcaccacaa
    16141 atcaacaccg tactgtcgct cttcgccaca atatctacct gctggagcag gtcttccatt
    16201 gcctggcttt gatagataat gtcaccgacg cccagattcg aaaggagctg ctcgctaagc
    16261 tggtggtttt cttgctgcaa actttcctgc aaatcggtca tgctacgcca ggcatcggca
    16321 ttgccaacgg caatagcgat gcgatcggct atgtgttgta gtagctggca gttttcctca
    16381 ctaaagagag tggtagaggt atgcgccagc aacaatgcac ccggtgtatg gttgccaaag
    16441 gtaagcggta tcaggagcga agattcgcag ccgttagatg caagtaagtg taataacggg
    16501 tcgcgttgcc agagaaacag atcgtctgca cggtgcgtca aggtcggttg tcgggtttgt
    16561 agcgtttgtg tcagcaatac actgtcgcca ggcatattgc gtggcagaca cgcacaatgt
    16621 gaggcagaaa gatcgctgca ccacaggctg aacttctcgt tctttcgatg atcgcccagt
    16681 accatactga ctgaagccag accgaaaaaa tgatggatct cacgagcgac gtcagcgatc
    16741 agatcgtcga gatcaagatg tgacagcact gcattagtga tatccaccag tacccgatga
    16801 tgatcgcgat ctttctccag caaacgcagc gccacctgtt gttcttcatc attgagccat
    16861 gattgcagaa ccggggtgat aatgcgtgcc agccagttaa acagtgtgca atcctgggga
    16921 gtaaaacgac tgccgtgggt gcgaataaag cgtagctccg ccattccact actgccgaga
    16981 aggacggcgt gacgcgaata atcgaccatc tcgccctgat actctgttgt tgccgtcaca
    17041 tcgctgccat catcatggta gcggtaatag aagttgcttt tgagaacatg cagttcaata
    17101 agttgtaccg ggctgaacgg cggcggcagc gggatgaagg cgcgtaataa agacgccttg
    17161 ccgtgtttct gcgctaaccg ctctgcgagc attccgttta ccgcttcaat tgttattcct
    17221 tgcggcgggg caaacatcgc ctcgtctgac atagccatca acagtcctta ttaatgagaa
    17281 atccttctca cgcaattttt ctggccatca gatagatagc cggttccagc acaatttcat
    17341 ccaacagatg aatgaattga atgctacgcg cttgccacac agacgtcgcg tcatctgcca
    17401 gtggcgcgaa tgcccgggtc aacagggcgg tgtggctggc gtcaataaca atctcaccaa
    17461 aagtcatcac ccccagcatc ttacgtcggg cttgctcctc ttcgaccagt gcaagccaat
    17521 cttcgtattc cgtcagtggg cagcggaagg tgacattcag acaatcaatc acgccaacgt
    17581 gatgaccgat agccagcgag taatacatta cctgggagct gtgttccggc atctcgtcgc
    17641 tactggcgac aaactttttt cgcagcgtcc agaaaacaat ttccccgcac tcttcagtca
    17701 tgtaaccccc ggagtacgcg aaagagttgc tgaacgatac tattgagacg tggatcgtcg
    17761 gcatcgcgca accaggtgtt aacccgattt cctgtaggat cgttgcttaa atggcgcagg
    17821 agccgatcgc aaatttcttg cccctgacga tagcccgcca gccgccgcgc ttctcgctca
    17881 atggcgatac gctgtgatgg cgggatctgc ggccacaacg gttgcatagt caccccggtg
    17941 ggatcgcgat aatccaccgc gtgaatcttc tgttgcagca aaccgagcgc cacggcgaaa
    18001 ccgtgaatgg tggcggccgg tgttggcggg cagccgggga tccaaacatc aatggggaca
    18061 atggtgtcgc tgccgcccca gacgctgtag agatcgtgga aaataccgcc gccgacaccg
    18121 cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca
    18181 ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc gtgacgcggt
    18241 gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata cgggtgtgat ggcggcaaaa
    18301 atttcgattt cacaggcgtt gcagccgccg caatcgacgc gataaacgta ggctgagcga
    18361 cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag cgtgatgggc
    18421 tggctgacat gttgtgtaag cactggactc atagctgctc cttagccacc agcggtacat
    18481 ctgtatcgtc gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg
    18541 cccgcaacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca
    18601 gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga
    18661 acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa
    18721 gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata caacgcccca
    18781 gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc agcgcatttg
    18841 tcggacaagc acaggcgcag gcaccacagg caatacattg gctgggcatc aggtccggtt
    18901 tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc gtcgcggttc
    18961 cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc agcggcgaac
    19021 ggttacgatc aatgccgtag cgttcgatct ctttatacgg cacggttttt gactggcgct
    19081 tgcgcacatc aaccagcgtc acacggtcgg tacaggagta gcagggatca aggctaccga
    19141 taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac accggccagt
    19201 tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt ttggttatca ccgagcatgc
    19261 tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc gagcgcgaat gcgtgaggtt
    19321 tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgaga gcaaattcca
    19381 gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag aaaacatcgc
    19441 cgccggtgaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag gggtgatcaa
    19501 agcgcaaatc acgggcaaaa ccactgccgc ggatcagcgg ccctacaggg ctataatcac
    19561 gggcgatttg tcggtcgaga atgccaatgc cctgagtgcg ttgttccata ttcggcgtgg
    19621 caagcagcat ttctaccagc tccgacacgt cggcgcgcat ctcgcgcacc agtttcagcg
    19681 tttgcagacg ttgctctttg agaatatcgc ggcgaacacc accaatcaga ttcagaccgt
    19741 aggttttacg cgacccggtc agcaattccg ccattgtcat cgacttttcc cgcacgcgga
    19801 aaaattgcat aaagccggta tcaaatccaa caaaatggca ggagaggcca aggttgagca
    19861 aatgactgtg tagccgttcg acttccagca gaatcgagcg aatggtatgc gctcgttgcg
    19921 gcacctcaat ccccagtgca ttttcaaccg agttggtata ggccacactg tgggcaaaac
    19981 cgcaaatccc acacacgcgg tccgataaga aggtcacttc gttgtagccc atccgcgttt
    20041 ctgccagttt ctccatgccg cgatggacat aaaacagacg gtaatcagca tcgacaattt
    20101 gctcgccatc cacaaacaag cggaagtgac ccggttcatc ggaggtgata tgcagcgggc
    20161 cgacagggat cacccgcgca ccgctgttgc cctcattgat aaacggatac gtttcggaat
    20221 cagtcgtcgg ttcagggcgc agtcgataat ccatcgcatc tttgcgcagc ggatgcatat
    20281 cttccggcca gtcatcgggc aacaccaggc gacgctgatc cggcaggcca accggaatca
    20341 gcccgtacat atcacgaatt tctcgctcgc cccagaccgc ggcagggacg cgcggtgtga
    20401 ctgacggaaa ctcccgactg tcggcatcga ccagcgcctt caccacaatc cagcattttt
    20461 cggccccttc cattgaaagg gcataataaa ccgcgtaatg accgttaagt gtccgctcgt
    20521 cgttgccaaa caggaccgga agccagccat catgttgata ataaagatac tgtacaacgt
    20581 caggcagcag attgattttc accgtaatgg tgacctgttc aggcgtttgt cgctcttcat
    20641 ccagcaccgc gccggggaac tgcgttttca gggcggcgag aatcgcttcg ccacgatttg
    20701 acgatgaatt aacgttcact gtgtttctcc gaaacagatg cggtgcctga aggcaaaaaa
    20761 tcatgccagg tgctacgttg tgcaggcagg tcgtgcgtcc ctgagaggac tatagtggaa
    20821 gcgcccgcca ggatcctgat gacaggttgt ggaatatgcg ttcccatcgc cagcatcatg
    20881 accagcagaa tcaccattgg cgaggtggtc aaccagccga gatcaccccg gttaacggcc
    20941 tgcggcggtt tcgccattaa cacccgcgca gccatccgta ccaggcccgc cagcaccagc
    21001 gttaacagca ataacagcag gacgataatc agcaggtgat tacgtgccag tccggcggta
    21061 acggtcataa attcgctaag aaaaatgttg aagggcggca tccctgccag cgccagcgca
    21121 ccgccgccaa acagcacggc ggtaaatggc atgattttga gcatcccaca gacgacgttg
    21181 agatcgcgcg tgccgtactt gagcagtaca ttgccggaac cgcagaacag cagcgttttt
    21241 gccagactgt ggtttaaggt gtgcagcagc gcggcaaaaa ttcccagcgg cccgccaatg
    21301 cctagcgcca ccgcgaccag tcccatgttc tccacgctgg agtacgccag cagacgctta
    21361 atgtcccgct gtaccagaat gaaaaatgcc gccacggcaa ccgacaacat gccgaagatg
    21421 agcaacaacc ggttggggaa atcgctgccg atggcctggc aaataatgat gtaatagcga
    21481 atcagcacca acagcgcgca gttcagcaat acggcggaga gtagggcgct gaccggactc
    21541 ggcgcttcac tgtgagcatc cggcagccag gcgtgcatgg gaaatagccc ggttttggta
    21601 ccaaagccaa ttagcacaaa cacaaaggcc aacagcatta atgttggatc aagcaaggac
    21661 gattgcttaa gaacctcgct ccagaatatc gccatttctg cctgcggcat aacgctggcg
    21721 gcgttggcgt ataccagcac ggtaccgaac agaccaaaag cgacagcaac agtacaaata
    21781 atgatgtact tccatgctgc ttccagcgat gaacgctgac cgtaaatgcc taccagaaac
    21841 gccgagctta aggtggtggc ttcgatcgcc gcccacatca caatcaggtt attgctggta
    21901 acaaccagca gcatggtgaa caaaaacaga tggaagaaac cgtagtaatc gcacagcgta
    21961 acgggtgaaa gctcgccgtg ctcaacttca tgacgcatgt agccaatcga gtaaacaccg
    22021 gtgagaaagc caatcacacc aagaatggcg aggaacaaac cgcccagacc atcaatatga
    22081 agccacagtc ccgcagcgaa tatttctcct gcatcagcgg cagtttggac cacccagagt
    22141 gccagaatca gcagcagtgt gatccctaag ctatgtaata ctgtcaccgt gcgagtcgca
    22201 gagtgccctc gtttccggca ggcaaaacag agcagcgaaa aaagcagcgg agtgagcagg
    22261 agtaaagcga acatcacaga ataactcatc tcattatccc ttcagcgcgg tcaagttgtt
    22321 cacgtccagc gtgccgtggg tacgccatat ttttcttgcc agtaacacca tcacaatgac
    22381 ggcgaagatg gcgtcggtag cgataccaat ttccaccagt tccggtgcac gccaggctag
    22441 aagcgccagc accagatggg agccgttttc catcaggcag taaccaaaaa tttgccgcag
    22501 gatattgcgc tggctaacaa tgcacagcag gccaagcaga aaatgaccta acgccaccgc
    22561 cagcgctggt ttcagcccgg tagccatcgg tagcttcacg ggctgaacga caaatgcgca
    22621 aagcaggaca attaacgccg cgagcagtgc catcattgcc ggaccgaata acgctttttc
    22681 cgggatgttc tggggaatat ttcgtgcagc gtaagtcatg attaacggta ccagcagcac
    22741 tttggtgata aaggcgctgg cggaccagat cagcagttgc tctgcggcga acaggcacga
    22801 gagggtggcg aaaatagaca ccagcaccag cgactggcag gcgtaaaatc cgcatgacag
    22861 gcgatagctt ttgacgctaa tcacaaacag cgatgtcagc atcatcagtc ccgccagatt
    22921 atttacgatc atagaaccgg tcattcatca tctccttact gcaaccaggt cgcggcgatt
    22981 acgctggaaa tcagcgacat cacaatcagt acaatcagca ccaggcgcat tgaccctggc
    23041 agcggtgcgg catcggcgac ggcctcgctc ggtttgccgg ggacgacgcg accaaaccag
    23101 cgaataaacc aggcgaaact ggcgaccgat tcaatcatca gcagaatcat ggcgggcagc
    23161 aggatccagt actccactga caacgcaaaa ccggcagcaa acagcgggaa tttactgaag
    23221 aagccgttga acggcggtac gccggtaatc gccagcgctg ccacgcagaa acccacgcct
    23281 ggcaacggca gggtgtgcaa tacgccacgc agacgcggca acaagcgcgt gccgcagctg
    23341 taactcagcg cacctgctac aaggaaaaac aggcttttag cgaacgcgtg gttgacgatg
    23401 taggcgatgc tgccctccag cgccagccgc gagccgaaga tggagagcga caagccgaag
    23461 aacatccagc caagttgagt gatggtcgac caggccagca accgcttcat atcctgctgt
    23521 ggcaaataca tcagaaatcc ataaagaatg gtgaccaggg ccatgaccat gccaacgccg
    23581 ccaatcacat gcgggatatt gccgccgtcg ataatggcgc gggcaaaaat gtaaacgccc
    23641 actttcacca tcgatgcggc gtggagatag gcgctgatcg gtgttggcgc ttccattgcg
    23701 tccggtagcc acgcttgcat cggtagctgg gccgatttcc cccacgcggc aaacaggatg
    23761 ccgccataaa ccagataacg tgcgtcgccg tgtaactcgc tcatcgcgct aagcgcaaac
    23821 gttccggtct gcaaaaacag cgtggcggcg gcaagataca accccaacga accgatatgg
    23881 gtgataagca acgcttttag tgctgaacgc tgcgctttat cgctctgata ataactgatc
    23941 aacacccagg agcagccgcc cgtaatttca aaaaacaaca actgaccgag cagcgtcgag
    24001 gagagtacca gtcccgccat cgcgccgata aacaccagca aaaatgcgta ataacgattc
    24061 gtgccgttat gcgggtgttc gcgattttta tccgtcagat aacccgtgga gtagatcgtg
    24121 accagcaaac cgagaaacac caccacaaac agaatcagcg tacacacgcg atcaatgacc
    24181 aggccaaaca atgccacatc ccccacgttg accaacgtca acgtgacggc aactttatcg
    24241 gcctgataaa acgcggagat cagcgacaac atgcacagcg tggtcagcgc ggcgaacaaa
    24301 accccccatt cggcggcccg acgttgtggc gaaaacgaaa cgaccagtgc gccgataaaa
    24361 ggcagcaata acgtcgtcag agcaagattt tccatattcc gtcagtgctc cttacagacc
    24421 ggttaaccag aagacccatg caagcgcagc aaggctgaag ccaagccagg tcacatggtg
    24481 aatgagtaaa aaacgcccgc gtgccagcgt gttttctgcg attgaggcca gtacaaaaat
    24541 cagcaaaacc ttgagcagcg taacgacaag tgaagtcagc aggcaggcga gagaaagttc
    24601 ttgcgcgcac ccaaagggca gaaacagggc cacaaacagt gctgccatca cgacctgttt
    24661 cagccccagc ccccatttcg ccagcgccag cccggcaccg gaatattctg tcagcgggcc
    24721 ttcctgtaat tcctgttctg cttcagcaac atcaaaggga atttttccca tctcaatgaa
    24781 gcaggcaaaa ccacaggcca gtaacgccag tacggtggtt agcggcgagt tccagcccgt
    24841 tgccagcgta ttgctgatca tctcgatatg cgtggaacct gctatcagcg ccaataccag
    24901 cagtgagaga atgagcattg gttcgaccag aatgccgagc gtcaactcgc gactggcacc
    24961 gactcccgca aacgggcttc cggtatccag cccggaaaga gcaaagaaaa aacgaaacag
    25021 ggcaagaaga tagataaggg tgatcagatc gccgccgccc gcaaaagggg aaacggtaat
    25081 aaatagtggt agggccatcg ccagcaccag catgctgctg attaataccc acggcatcag
    25141 gcggaacatc agacctgaag atataggcgc aacttcctgg cgtttaaaca gtttgtggat
    25201 atcgcgataa tcctgccaga tccccggccc gcggcgggag tgcatacgcg cgcgtatctg
    25261 ccgggaaata cccgtaaaaa gcggggttag catcagcaga atcacggcct gtgctaacgc
    25321 aaaaatgacc agatatccgt cgcaaagagt ttgtctcatg gtgatttcct tagacggcaa
    25381 tagcgattag cagcacaacc agtgcggcga ccacatacag gcaatagaga cgaaagtcgc
    25441 cgctttgcag atgctgtatt tctttggcca gccgttgggt ggcgctcacg atggggcgga
    25501 tcacccgctc atcccagaag ggttctgtgc tctgagccct ggcggtgacg tgcgcaagac
    25561 ctttatttag cctcagcgta gggtcgagct gttgtcgtag acgaaatagc gcagagaaga
    25621 ccacacgcag cggctgcatc acgccattgc ctgacggggc catcgcattt tcccagccgt
    25681 agccgcatgc ccaggcatct cctgtgcgac gaaacgctgc gcggcgcgaa cgacaccaca
    25741 gccagtaaag gccaggcagc aaaggcatcg ccagtagtaa cagcaacagt aatgaggggg
    25801 tgacctgtgt atgaaacgtg ccgggtacaa gtgctattcc gctggcgaca gtgacgggag
    25861 gggtattggt aaacgcatgg gcaatatgca ttatcttcgg tgccagccaa ctggcactaa
    25921 gcgcaattaa tacgcagagt gccgcgagca gtagcatggc gacgatcatc gtatttggca
    25981 cttcctgtgc ctcttcagcg tgtgtactgc gcggcgcacc acagaaagta ataccgtaca
    26041 ttttgacgaa gcacattact gccagcccac cagtgactgc cagcatcacc atagcaatag
    26101 gacccgcaag ttgtagcgct acggcttcca cacgacttag tgagaacagc gactgccagg
    26161 tgtaccattc gctgataaaa ccattcagcg gaggaagggc tgatatcgcg aggcaaccaa
    26221 tcaggcatgc tgcggctgtc cacggcatcc gtttcgccag tgcccccatt ttttccatgt
    26281 cgtgggtatg caaacgcgaa ataatcgcgc ccgcgccgag aaatagcagc cctttgaaca
    26341 gcgcatggtt gagcagatga aacagtgcgc cgagcagtcc aaccacggtg agcagcgggt
    26401 cgtgtagtga cagaccgacc atcgccacac cgactgccag caaaataatg ccgacgtttt
    26461 cgacggtact ccaggccagc agccgtttga tatcctgttc cgccagcgca tacagcacgc
    26521 ccaggagcgc ggagattgcg ccgatcgcca tcaccagaat gccccaccac agcggcaaac
    26581 ccgtttgcgc cagcagatcc atcgccactt tcaggatgcc gaaaataccg attttgacca
    26641 ttacgccaga catcaacgcc gaagcgtgcg atggtgcggc agggtgagcg cgcggcaacc
    26701 agctgtgcaa cggcatcatc ccggctttcg cgccaaaacc gaaaaaggcc agcaggaaca
    26761 ccgccgacgc cagccccgga gaaagtgaaa gcgtgcggaa actggcaaaa tctaggctgc
    26821 cgctttcgcg ccccatcagc aagaaggcga tcataatcag caccgatccg gcgtgggcga
    26881 taaaaaagta gagcatgcca gcgttgatcg acgttttatc ctgcctggca atgaccagaa
    26941 accaggaaga cagcgacatc atttcaaaca gcacgatgaa ccaaaaagcg ttgtccatca
    27001 ccagcagggc aaccatcgat gcgatgaaaa tattcataaa gaagcccatc gccgccgcgc
    27061 ctttgccctc gtattcgcgc atataagtca atgaatagag cgaacaaacc accaccagca
    27121 aggagataac cagcaccata aatgccgaga ggctatccat tcgcagggtt aaatcggcaa
    27181 agtcaaaagg ggtggcaaaa cgggcaacta aagttacgcc ttcatgtaat tgcgtaattc
    27241 cgctgatgat gccaatcacg ccgcccacca gcgatgtgat gccggaaagc ttaatagcca
    27301 gtctgtccag accgagtaaa aacagcgaag ccagactggc aaacagatag aggatcagcg
    27361 accaggttaa taattgcagg gcatccatta gcgttgctcc tgagtgaggg aaaggggatc
    27421 ctccctgcca ttggcaaacc agcttgcggc aaggcgctgt ttttctttca tctgacgttg
    27481 caggctatcg tcggtgatca gccgtaacgc ctgattcggg caagcgcgaa cgcaggcagg
    27541 accttctggc aagaaatcac acaggtcgca tttcaccgcg acggtctgga cacccggttc
    27601 ccagcgcagc aaagcatgtt gtgttggcgc gttttcttcg ccgtctttga gtgagccttc
    27661 agcctgaaaa acatattgcg caggggcatt caccggacgg cttcctgaag cgctgattgc
    27721 gccaaatggg cagaccacgg cgcaaagctt gcagccaata cagaggcttt cgttgagttg
    27781 gatcgcatca tccctctggg agatggcatt gaccgggcag acctgcacgc aaggggcttc
    27841 ctcacagtga tgacacacga caggggcagt gagtgttgac gtcttcgcca gggccaggcg
    27901 cgggtgttgc tgtaaacctt gcgttttatg cacgtccgaa caggcagcga gacaggtatt
    27961 acatcctgta caccacagtg gttcggccac cacaaagcgg ttcatgtcgg ctccttgaaa
    28021 atgatatgaa acattgcatt tcataatgca taaaggatgc cagtatgatt aatttaatat
    28081 attcaataag ttatgaatta actgaattct gaacggtaca aaaatgatgg tcattaacgt
    28141 tcagcaaaaa atggattcga cacccgtgtc gcaggggaaa gtgagaatgt gatcttgatc
    28201 acgataagtg aggtgtgctg accgcaagcg cagccagcac ggaatggagc aaagtaatca
    28261 ggcgtgttct ttcagccagt tcagcacaac gtcgtggtga ttgctggttt tgaaatcgtc
    28321 aaagacatgt tcgattttgc catcagcgtc aatcaggaag ctgatgcgat gaataccatc
    28381 gtaggttttg cccatgaagg acttttcccc ccagacgccg aattgctcgc acacctggtg
    28441 gtcctcatca gacaggagcg taaagttaag cagctctttt tccgcaaaac gggagagttt
    28501 ttcgggttta tcggtgctga tacccagcac atcaacgccc gcttttttca actcatccat
    28561 gttatcgcgt aagccgcagg cctgtacggt acagccgggg gtcatggctt tcgggtagaa
    28621 ataaaccaga acacgctgtc cctggaagtc ggtcaaatta acttgttctc cgtcttgatc
    28681 cggcaagcta aatttcggtg cgatatcacc ggctttcagt ggattcatta cttaactcca
    28741 tcctgttcat catgttggga ataattgacg acgttaatac tgccttgtgc attgagttct
    28801 gtacataggg ctttgaacgc ttgctcaata tttgctgcgt ccgcagatgc ggggctgtgg
    28861 gcagtaatct gaatatgcaa ctgcgcagcc cgttcatttt cagcaggttg tgtgcgcgac
    28921 accagctccg caatgttcat atgatgcgcg tcgaaaagtg ctgtgaagcg ttcaattaaa
    28981 tgcggggagt ctgccacatc gacctgaacc cagacagatg ctggcattgg cggacgcgga
    29041 cgcgccgtcg tgcgcttcat cacgattaaa agatccagtt cggcaccttt caacggtaac
    29101 gttgattcaa tcagagtaat ggcattccat gaaccggaaa gcagcatgat aaacgtgaac
    29161 tcttctccca gcatcgccag gcgactgtct tcaatattac agccgcaact actgacatga
    29221 cgggtgatgg tgttcacaat tccagggcga tcggcaccca acgcagtgat caccagataa
    29281 tgttgcgatg acagtgtcaa actggttatt cctttaaggg gtgagttgtt cttaaggaaa
    29341 gcataaaaaa aacatgcata caacaatcag aacggttctg tctgcttgct tttaatgcca
    29401 taccaaacgt accattgaga cacttgtttg cacagaggat ggcccatgtt cacgggaagt
    29461 attgtcgcga ttgttactcc gatggatgaa aaaggtaatg tctgtcgggc tagcttgaaa
    29521 aaactgattg attatcatgt cgccagcggt acttcggcga tcgtttctgt tggcaccact
    29581 ggcgagtccg ctaccttaaa tcatgacgaa catgctgatg tggtgatgat gacgctggag
    29641 ctggctgacg ggcgcattcc ggtgattgcc gggaccggtg ctaacgctac tgcggaagcc
    29701 attagcctga cgcagcgctt caatgacagt ggtatcgtcg gctgcctgac ggtaacccct
    29761 tactacaatc gtccgtcgca agaaggtttg tatcagcatt tcaaagccat cgctgagcat
    29821 actgacctgc cgcaaattct gtataatgtg ccgtcccgta ctggctgcga tctgctcccg
    29881 gaaacggtgg gccgtctggc gaaagtaaaa aatattatcg gaatcaaaga ggcaacaggg
    29941 aacttaacgc gtgtaaacca gatcaaagag ctggtttcag atgattttgt tctgctgagc
    30001 ggcgatgatg cgagcgcgct ggacttcatg caattaggcg gtcatggggt tatttccgtt
    30061 acggctaacg tcgcagcgcg tgatatggcc cagatgtgca aactggcagc agaagggcat
    30121 tttgccgagg cacgcgttat taatcagcgt ctgatgccat tacacaacaa actatttgtc
    30181 gaacccaatc caatcccggt gaaatgggca tgtaaggaac tgggtcttgt ggcgaccgat
    30241 acgctgcgcc tgccaatgac accaatcacc gacagtggtc gtgagacggt cagagcggcg
    30301 cttaagcatg ccggtttgct gtaaagttta gggagatttg atggcttact ctgttcataa
    30361 gtcgcgcctg gcaacggttg cgggtgtttc gcttgtttta ttactcgctg cctgtagttc
    30421 tgactcacgc tataagcgtc aggtcagtgg tgatgaagcc tacctggaag cggcaccgct
    30481 tgcggagctt catgccccgg ctggaatgat tttgccggtg acctccggtg attatgcaat
    30541 cccggtgacc aacggtagtg gtgctgtcgg taaggcgctg gacattcgtc caccagccca
    30601 gccgctggca ctggtttctg gcgcgcgtac ccagttcacg ggcgataccg cttcattgct
    30661 ggtggaaaat ggtcgtggca atactctgtg gccgcaggtg gttagcgtgc tgcaggcgaa
    30721 aaactacacc atcacccaac gtgatgatgc tggtcagaca ctgaccaccg attgggtaca
    30781 atggaaccgt ctggacgaag acgagcagta tcgtggtcgt tatcaaatct ctgttaagcc
    30841 gcagggttat cagcaggcgg ttacggttaa actgctgaac ctggaacagg cgggcaaacc
    30901 ggttgcagac gcggcttcca tgcagcgtta cagcacggag atgatgaacg ttatttccgc
    30961 tggtctggat aaatctgcca ctgacgccgc gaacgctgcg caaaatcgtg cctccaccac
    31021 tatggacgta caaagtgcag ctgatgacac cggtttacca atgctggtcg tacgcgggcc
    31081 gttcaatgtg gtttggcaac gtctgccagc ggcgctggaa aaagtgggca tgaaagtgac
    31141 cgacagcacc cgttcgcagg gcaacatggc cgtaacttat aagccgctgt ctgacagcga
    31201 ctggcaggaa ctgggcgcga gcgatccagg cctggcatct ggtgactata aactgcaggt
    31261 tggcgattta gataaccgca gcagcctaca gttcatcgat ccgaaaggtc atactctgac
    31321 tcagagtcag aacgacgcgc tggtagctgt cttccaggct gcgtttagca agtaaaaata
    31381 cagggctgga atcatccggc ccttttttct gatatgatac gcaaacgtgt gcgtcggcag
    31441 gaaaacgcga ttttagcggt aattcgcacg aaatttgttt gtcggacgta gttcggataa
    31501 ggcgttcacg ccgcatccga caaaacatcc ggcacaccag acagcaaaag attttaaaac
    31561 gttaattcac acccaggagt gataaagatg caaaagcaag ctgagttgta tcgtggtaaa
    31621 gcgaaaaccg tatacagcac ggaaaacccg gacctgttgg tgctcgaatt ccgcaatgat
    31681 acgtcagcag gggatggcgc gcgcattgag cagtttgatc gcaaaggtat ggtgaacaac
    31741 aagttcaact acttcattat gagcaaactg gctgaagcgg gtatcccgac tcaaatggag
    31801 cgtctgctct ccgataccga atgtctggtg aaaaagctgg atatggtgcc ggttgagtgt
    31861 gtcgtgcgta accgtgctgc tggctctctg gtgaaacgtc ttggaatcga agaaggtatt
    31921 gagctgaacc cgccgctgtt cgatctgttc ctgaaaaacg acgccatgca cgatccgatg
    31981 gtcaacgaat cttactgcga aacctttggc tgggtaagca aagagaacct ggcacgcatg
    32041 aaagagctga cctacaaagc gaacgacgtg ctgaaaaaac tgttcgatga tgctggtctg
    32101 attctggtcg acttcaagct ggagtttggt ctgtacaaag gtgaagtggt actgggcgat
    32161 gagttctccc cggacggtag ccgcctgtgg gacaaagaaa cgctggagaa aatggacaaa
    32221 gaccgtttcc gccagagcct cggtggcctg atcgaggcct atgaagccgt cgcccgccgc
    32281 ctgggtgtac agctggactg atttttctgt tcatcatctt gccgtgctgc tggcacggca
    32341 agacaaccat tctcgtaaga tgtgcattga acgctcattc ctccgccatt tcatcccgtt
    32401 ttcctgtggt aatcttcctc tgaaccacct acgatcattt ctataatgaa tatattgttg
    32461 aggtatctat gcgttggcaa gggcgacgtg aaagtgacaa tgttgaagac aggcgcaata
    32521 gctccggcgg tccgtcaatg ggcggtcccg gtttccgtct gccgagcggt aaaggcgggc
    32581 tgattttact gatagtcgtg ctggttgcag gctactatgg cgttgattta accgggttga
    32641 tgaccgggca gccggtttct caacagcaat caacgcggtc aattagccca aatgaagacg
    32701 aagccgcaaa attcacctcg gtgattctgg caaccacgga agacacctgg ggacaacagt
    32761 tcgagaagat gggtaagacc tatcagcaac cgaagctggt catgtaccgt ggaatgacgc
    32821 gtaccggatg cggggcgggc cagtccataa tggggccgtt ctattgcccg gcggatggta
    32881 cggtttatat cgatctctcc ttctatgatg acatgaaaga caaacttggc gcggatggcg
    32941 attttgccca ggggtacgtt atcgcccatg aagtcggtca tcatgtgcag aaactgttag
    33001 gcatcgagcc gaaagttcgt caactgcaac aaaacgcgac gcaggcggaa gtgaaccgct
    33061 tatctgtgcg tatggaactc caggccgact gttttgccgg tgtctggggg catagcatgc
    33121 agcagcaagg cgttctggaa accggcgatc tggaagaggc gctgaacgcg gcgcaggcca
    33181 tcggcgatga ccgtttacaa cagcaaagtc aggggcgagt agtaccagac agtttcactc
    33241 atggcacttc tcagcaacgc tacagctggt ttaaacgtgg tttcgacagc ggcgatccgg
    33301 cacaatgcaa tacttttggt aaaagcattt aacttccggg gcagggatgg ctgaactgac
    33361 tgcgcttcac acattaacag cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt
    33421 gagcggggaa gagggttggt gttttgatca tgcgcttaag ttacgtgatg ccttacctgg
    33481 cgactggctg tggatttcgc cgcagccaga tgctgaaaac cactgttctc cctcggcact
    33541 acaaacttta cttgggcgcg agttccggca tgcggtattc gacgcccgcc acggctttga
    33601 tgccgctgcc tttgccgcac ttagcggaac gttgaaagcg ggaagctggc tggttttgtt
    33661 actccctgta tgggaagagt gggaaaacca acctgatgcc gactcgctgc gctggagtga
    33721 ttgccctgac cctattgcga cgccgcattt tgtccagcat ttcaaacgcg tacttacggc
    33781 ggataacgac gctatcttct ggcggcaaaa ccagccgttc tcgttggcgc attttactcc
    33841 ccgtactgac tggcaccccg cgaccggcgc accacaacca gaacaacagc aaatcttaca
    33901 gcagctactg accatgccgt cgggcgtggc agcggtaact gctgcgcgtg ggcgcggtaa
    33961 atcggcgctg gcagggcaac tcatttctcg tattgcgggt agtgcgatta tcactgcgcc
    34021 cgcaaaagcg gcaacggatg tactggcaca atttgcgggc gagaagtttc gctttattgc
    34081 gccggatgcc ttgttagcca gcgatgagca agccgactgg ctggtggtcg atgaagccgc
    34141 agccatacct gcgccgttgt tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac
    34201 cactacggtg cagggctacg aaggtaccgg acgtggtttt ttgctgaaat tttgcgctcg
    34261 ctttccgcat ttacaccgtt ttgaactgca acagccgatc cgctgggcac agggatgccc
    34321 gctggaaaaa atggttagtg aggcactggt ttttaacgat gaaaacttca cccatacacc
    34381 acaaggcaat atcgtcattt ccgcatttga acaaacgtta tggcgaagcg agccagaaac
    34441 gccgttaaag gtttatcagt tattgtctgg tgcgcactac cggacttcgc cgctggattt
    34501 acgccgcatg atggatgcac cagggcaaca ttttttacag gcggctggcg aaaacgagat
    34561 tgccggagcg ctgtggctgg tggatgaggg gggattatct caagaactca gtcaggcggt
    34621 atgggcaggt tttcgtcgcc cgcggggtaa tctggtggcc cagtcgctgg cggcgcacgg
    34681 cagcaatcca ctggcggcga cattgcgtgg acggcgggtc agccggatag cagttcatcc
    34741 ggcgcgtcag cgcgaaggcg ttgggcaaca gctcattgcc agcgctttgc aatataggcc
    34801 tggcctcgac tatctttcgg tgagttttgg ttacaccggg gagttatggc gtttctggca
    34861 acgctgcggt tttgtgctgg tgcgaatggg taatcatcgt gaagccagca gcggttgcta
    34921 tacggcgatg gcgctgttac cgatgagtga tgcgggtaaa cagctggctg aacgtgagca
    34981 ttaccgttta cgtcgcgatg cgcaagctct cgcgcagtgg aatggcgaaa cactccctgt
    35041 tgatccacta aacaatgccg tcctttctga cgacgactgg cttgaactgg ccggttttgc
    35101 tttcgctcat cgtccgctat taacatcgtt aggttgctta ttgcgtctgc tacaaaccag
    35161 tgaactggca ttaccggcgc tgcgtgggcg tttacagaaa aacgtcagcg acgcgcagtt
    35221 atgtaccaca cttaaacttt caggccgcaa gatgttactg gtccgtcagc gggaagaggc
    35281 cgcacaggcg ctgttcgcac ttaatgatgt tcgcactgag cgtctgcgcg atcgcataac
    35341 gcaatggcaa ttttttcact gactccttca attttctccc atggtcattg tgagtcagcg
    35401 gcgtaaaatt aatgacatca gttaaggagt tcgccatgaa acatgaccat tttgttgttc
    35461 aaagcccgga taaacctgcg caacagttgt tactgctttt ccacggtgtc ggggataacc
    35521 cggtggcaat gggggaaata ggtagctggt ttgcaccgtt gttcccggat gcgctggtgg
    35581 tgagtgtcgg tggcgcggag ccgagcggta atccggcggg gcgtcagtgg ttttcggtgc
    35641 agggtattac ggaagataat cgccaggcgc gtgtggatgc catcatgccg acgtttattg
    35701 aaacggtgcg ctactggcag aaacagagcg gagtaggggc taacgccacg gcgctaatcg
    35761 gtttctctca aggcgcgatt atggcgctgg agagcattaa agccgaaccg ggccttgctt
    35821 cacgcgtcat tgcttttaac ggacgctatg cgagtctgcc ggaaaccgcg tctacagcca
    35881 cgacaattca cttgattcat ggcggtgaag atccggttat cgatctggcg catgcagttg
    35941 cggcgcagga agcgttaatc agtgccggtg gtgacgtgac gctggatatc gtggaggatt
    36001 taggccatgc gatcgacaat cgcagtatgc aatttgccct ggatcacttg cgttatacca
    36061 ttccgaaaca ttatttcgac gaagcgctaa gcggcggtaa gcctggcgat gatgatgtga
    36121 ttgaaatgat gtaataatag ccgggtaggc ctgataagac cgtatcaggc atcatggccg
    36181 gatgcggcgt taacgcctta tccggccact tagcttactt cttcggttga tcctttttcg
    36241 gccagtcgtc gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct
    36301 tgttggcgag aaatttctta tggtcgacgc gcttgagatc tttaatcacg ttcagcagga
    36361 cgcctaccaa aaagacaatc accagaatcc accaatattt agccagccag tccatgctta
    36421 tttcctcttg cagaaccact catcacgcga cgagctgttc catgatacgt tgatacatac
    36481 gggcaagtag ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat
    36541 tgaccggccc gagttccacc acctgcgccc ccatgcgggc aataaagcgc ccgtcggacg
    36601 ttccgcctgt ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga
    36661 ccgcatccac cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccaatcca
    36721 ccgtatagcg cagttgatgt ttttcaagca gggcaagcac ctgcgctttg atcatctcat
    36781 cagtcagttc ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacgt
    36841 tgttactgcc cgttcccgcc tgaatattgg caatctgcat actggtcgcc gggaagaatt
    36901 cattgccctg atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta
    36961 ccggattgtc agccagatgt gggtaggcaa catgcccctg aacgccatga atggttaggt
    37021 tgcaggttaa cgatccacga cgaccatttt tcaccacatc acctaccact tcgatactcg
    37081 acggttcgcc gaccaggcag taatcgagac gctcattacg tgccattaac gcttcgacgg
    37141 cttttaccgt accattgtgg gcactggctt cttcatcaga ggtgatcaga aatgccagtc
    37201 gccctgtatg gttgggatgt tgtgcgacaa aacgttctgc agctaccacc atcgccgcca
    37261 gcgagccttt catatctgcc gcaccgcgcc cgaataacat gccgtcacga atggttggtt
    37321 caaacggcgg attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg
    37381 caaaggccaa tgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt
    37441 ccatgcgttc aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc
    37501 ctgcgtcatc aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg
    37561 ggcacgacat agactacacc tcatggaaaa atttctgata actggaatca ctgaaaccca
    37621 gcagcatagg cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag
    37681 tcattaatgc cgccgcagag gccgcatcgg tgattatatt gcgggtggtt tcgtccagtt
    37741 tatgccaggt tgtaccacgg gtgttgagta acgcttccca gcctaattcg ttgataaaac
    37801 cgttcaataa ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt
    37861 tattggcttc cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa
    37921 gtgtaaccat aatgaatcct ttttcgattt atgagaataa atactcattt aagagtgaaa
    37981 acattttgcc tgcaaatgat tatcaggaaa t
//
LOCUS       DAZGXS010000029        37437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGXS010000029 DAZGXS010000000
VERSION     DAZGXS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(98..751)
                     /gene="ribB"
                     /locus_tag="ACHKG5_001638"
     CDS             complement(98..751)
                     /gene="ribB"
                     /locus_tag="ACHKG5_001638"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0867761.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(855..1003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1125..1424
                     /gene="ubiK"
                     /locus_tag="ACHKG5_001639"
     CDS             1125..1424
                     /gene="ubiK"
                     /locus_tag="ACHKG5_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0867762.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1459..1659)
                     /gene="glgS"
                     /locus_tag="ACHKG5_001640"
     CDS             complement(1459..1659)
                     /gene="glgS"
                     /locus_tag="ACHKG5_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0867763.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            1928..2542
                     /gene="yqiJ"
                     /locus_tag="ACHKG5_001641"
     CDS             1928..2542
                     /gene="yqiJ"
                     /locus_tag="ACHKG5_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0867764.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            2584..4245
                     /gene="yqiK"
                     /locus_tag="ACHKG5_001642"
     CDS             2584..4245
                     /gene="yqiK"
                     /locus_tag="ACHKG5_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0867765.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            4484..4543
                     /locus_tag="ACHKG5_001643"
     CDS             4484..4543
                     /locus_tag="ACHKG5_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0867766.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4860..4919
                     /gene="ibsE"
                     /locus_tag="ACHKG5_001644"
     CDS             4860..4919
                     /gene="ibsE"
                     /locus_tag="ACHKG5_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP0867767.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(5039..6472)
                     /gene="hldE"
                     /locus_tag="ACHKG5_001645"
     CDS             complement(5039..6472)
                     /gene="hldE"
                     /locus_tag="ACHKG5_001645"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0867768.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6520..9360)
                     /gene="glnE"
                     /locus_tag="ACHKG5_001646"
     CDS             complement(6520..9360)
                     /gene="glnE"
                     /locus_tag="ACHKG5_001646"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0867769.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQDEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(9383..10684)
                     /gene="ygiF"
                     /locus_tag="ACHKG5_001647"
     CDS             complement(9383..10684)
                     /gene="ygiF"
                     /locus_tag="ACHKG5_001647"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0867770.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10926..11546
                     /gene="ygiM"
                     /locus_tag="ACHKG5_001648"
     CDS             10926..11546
                     /gene="ygiM"
                     /locus_tag="ACHKG5_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0867771.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11610..12848
                     /gene="cca"
                     /locus_tag="ACHKG5_001649"
     CDS             11610..12848
                     /gene="cca"
                     /locus_tag="ACHKG5_001649"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0867772.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(13029..13850)
                     /gene="bacA"
                     /locus_tag="ACHKG5_001650"
     CDS             complement(13029..13850)
                     /gene="bacA"
                     /locus_tag="ACHKG5_001650"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0867773.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13941..14309)
                     /gene="folB"
                     /locus_tag="ACHKG5_001651"
     CDS             complement(13941..14309)
                     /gene="folB"
                     /locus_tag="ACHKG5_001651"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0867774.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            14415..15032
                     /gene="plsY"
                     /locus_tag="ACHKG5_001652"
     CDS             14415..15032
                     /gene="plsY"
                     /locus_tag="ACHKG5_001652"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0867775.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(15045..15977)
                     /gene="ttdR"
                     /locus_tag="ACHKG5_001653"
                     /gene_synonym="dan"
     CDS             complement(15045..15977)
                     /gene="ttdR"
                     /locus_tag="ACHKG5_001653"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0867776.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            16184..17095
                     /gene="ttdA"
                     /locus_tag="ACHKG5_001654"
     CDS             16184..17095
                     /gene="ttdA"
                     /locus_tag="ACHKG5_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0867777.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            17092..17697
                     /gene="ttdB"
                     /locus_tag="ACHKG5_001655"
     CDS             17092..17697
                     /gene="ttdB"
                     /locus_tag="ACHKG5_001655"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0867778.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17746..19209
                     /gene="ttdT"
                     /locus_tag="ACHKG5_001656"
     CDS             17746..19209
                     /gene="ttdT"
                     /locus_tag="ACHKG5_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0867779.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(19252..20265)
                     /gene="tsaD"
                     /locus_tag="ACHKG5_001657"
     CDS             complement(19252..20265)
                     /gene="tsaD"
                     /locus_tag="ACHKG5_001657"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0867780.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20503..20718
                     /gene="rpsU"
                     /locus_tag="ACHKG5_001658"
     CDS             20503..20718
                     /gene="rpsU"
                     /locus_tag="ACHKG5_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0867781.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20829..22574
                     /gene="dnaG"
                     /locus_tag="ACHKG5_001659"
     CDS             20829..22574
                     /gene="dnaG"
                     /locus_tag="ACHKG5_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0867782.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            22769..24610
                     /gene="rpoD"
                     /locus_tag="ACHKG5_001660"
     CDS             22769..24610
                     /gene="rpoD"
                     /locus_tag="ACHKG5_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0867783.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24689..25195)
                     /gene="mug"
                     /locus_tag="ACHKG5_001661"
     CDS             complement(24689..25195)
                     /gene="mug"
                     /locus_tag="ACHKG5_001661"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0867784.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            25320..25395
                     /locus_tag="ACHKG5_001662"
     tRNA            25320..25395
                     /locus_tag="ACHKG5_001662"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25353..25355,aa:Ile,seq:cat)
     gene            complement(25449..26213)
                     /gene="nfeF"
                     /locus_tag="ACHKG5_001663"
                     /gene_synonym="yqjH"
     CDS             complement(25449..26213)
                     /gene="nfeF"
                     /locus_tag="ACHKG5_001663"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0867785.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            26501..27124
                     /gene="nfeR"
                     /locus_tag="ACHKG5_001664"
                     /gene_synonym="yqjI"
     CDS             26501..27124
                     /gene="nfeR"
                     /locus_tag="ACHKG5_001664"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0867786.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(27278..28798)
                     /gene="aer"
                     /locus_tag="ACHKG5_001665"
     CDS             complement(27278..28798)
                     /gene="aer"
                     /locus_tag="ACHKG5_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0867787.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            29216..30595
                     /gene="ygjG"
                     /locus_tag="ACHKG5_001666"
     CDS             29216..30595
                     /gene="ygjG"
                     /locus_tag="ACHKG5_001666"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0867788.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(30637..30969)
                     /gene="ygjH"
                     /locus_tag="ACHKG5_001667"
     CDS             complement(30637..30969)
                     /gene="ygjH"
                     /locus_tag="ACHKG5_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0867789.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            31188..32171
                     /gene="ebgR"
                     /locus_tag="ACHKG5_001668"
     CDS             31188..32171
                     /gene="ebgR"
                     /locus_tag="ACHKG5_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0867790.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            32355..35447
                     /gene="ebgA"
                     /locus_tag="ACHKG5_001669"
     CDS             32355..35447
                     /gene="ebgA"
                     /locus_tag="ACHKG5_001669"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0867791.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            35444..35893
                     /gene="ebgC"
                     /locus_tag="ACHKG5_001670"
     CDS             35444..35893
                     /gene="ebgC"
                     /locus_tag="ACHKG5_001670"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0867792.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            35956..37389
                     /gene="ygjI"
                     /locus_tag="ACHKG5_001671"
     CDS             35956..37389
                     /gene="ygjI"
                     /locus_tag="ACHKG5_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0867793.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         9100 a        10125 c         9470 g         8742 t
ORIGIN      
        1 aagcggaatt aacatcttgc atagtaggga aaaataaaaa ccgaagcctg ctacaacttc
       61 ggtttcttga ttaaggcagt aaattaagca gcggttttca gctggcttta cgctcatgtg
      121 cctgacggta tgccaccagg tcttcaatag tcacgagcgc catgttgtgt ttattggcaa
      181 actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagca
      241 caccagccgg tttgaagcct gccagcgtca ttagatcaat agttgcttca gtatgaccgc
      301 cacgcgtcag tacaccacca gcctgagcgc ggagtgggaa aacgtggccc gggcgattca
      361 gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cggtcagcgg
      421 cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa ccggtgccat
      481 aggcgctggt gttattttct accatcattg gcagatcgag ttgtttacgg cgatcttcag
      541 taatgcacag gcaaacaata ccactaccgt ggcgaatggt cagcgccatc tgctcaacag
      601 tcatggtttc tgccgggaag atcatatcac cttcgttttc acggtcttca tcatcaagca
      661 ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg
      721 taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt
      781 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
      841 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
      901 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
      961 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
     1021 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
     1081 gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
     1141 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
     1201 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg cgtctcgatc
     1261 tggtaagccg cgaagaattc gacgttcaga cgcaagtcct gttgcggact cgtgaaaaac
     1321 tggcgttact ggagcagcgc agcagtgaac tggaagcgcg caataattcg gtagcagatc
     1381 ttcaatctcc tccggctatc ccgcctattg ataaagcaga ataaagcaac acaaaaacgg
     1441 gccagacggc ccgtaatatc agtgttctaa ttcaagttct ctggtttgca tcatctgttg
     1501 gcaataccag gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt
     1561 aacggccaga atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag
     1621 gaaatcgaaa ttatttaaag aattaagact atgatccata agcacatcct ccggttatcg
     1681 catacctttt aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc
     1741 tttaaacata acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa
     1801 aaccatatta aaagactttc tcttccagaa cctctctaac atatgttatt attaacaaag
     1861 acaaatgtaa gcaaggaaac tatcacaaag taaatattgc gtcactaaat ggacattgga
     1921 gtgacatatg attttattcg ccgactataa caccccttat cttttcgcta tttcctttgt
     1981 actgttgatc ggccttttag aaatttttgc gcttatctgc ggtcacatgc tctccggtgc
     2041 tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta gccaggcact
     2101 tcattatctc aatatcggaa gattgccagc tctcgtcgtt ctttgcttac tggctggttt
     2161 ttttggtctt attggcatcc tgttgcagca tacctgcatc atggtctggc aatcaccact
     2221 ctcgaacctg tttgtggttc ctgtcagttt actatttacg attattgcgg tgcattatac
     2281 cggtaaaata gttgcgccct ggattccacg cgatcatagc tcagctatta cagaagaaga
     2341 atatattggt agcatggcgt taattaccgg tcatcaagcc acttcaggta atccctgcga
     2401 aggaaaactc accgatcatt ttggccaaat acattattta ctactggagc ctgaagaagg
     2461 aaaaattttc actaaaggag acaaagtgtt aattatttgc cgactttctg caacgcgata
     2521 tttggcggaa aacaatccct agccccaaat attgtaattt attaatcaaa aggaaattta
     2581 ataatggatg atattgttaa ttccgtgccc tcctggatgt ttaccgcgat tattgccgta
     2641 tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc ttcggcagag
     2701 caagcatttg tccgtaccgg cttagggggg caaaaagtgg taatgagcgg tggcgcaatc
     2761 gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa gctggaagtc
     2821 agccgctcaa ccattgatag cctgattacg aaagatcgta tgcgcgtcga tgtagtagtc
     2881 gctttctttg tgcgggtaaa accttcagta gaagggattg ccaccgctgc ccagacgctg
     2941 gggcaacgca ccctgtcgcc tgaagactta cgtatgttgg ttgaagataa atttgtcgat
     3001 gccctccgtg caacagctgc gcaaatgacc atgcatgagt tacaggatac ccgcgagaac
     3061 ttcgtgcagg gagtacaaaa taccgtagct gaagatctgt cgaaaaacgg cctggaactg
     3121 gaaagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa tcctaacaat
     3181 gcctttgacg ccgaaggttt aaccaaactg actcaggaga cggagcgccg tcgccgcgag
     3241 cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg agaaaaaccg tgatgcactt
     3301 tcgcgcaagt tggagattga acaacaagaa gcgtttatga cgcttgagca ggagcagcag
     3361 gttaaaaccc gtaccgctga gcagaatgcg aaaattgcgg cttttgaagc tgaacgtcgt
     3421 cgtgaagcag agcagacgcg aattctggct gaacgacaga ttcaggaaac agaaatcgac
     3481 cgcgaacagg ccgtccgctc aagaaaagtt gaagctgaac gtgaagttcg cattaaagag
     3541 atcgaacagc agcaggtcac cgaaatcgct aaccagacga aatcgatcgc tattgccgcc
     3601 aaatcggaac aacagtccca ggcagaagcg cgtgctaacc ttgcactcgc agaagcagta
     3661 agcgcccaac aaaacgtaga aaccactcgc cagacagccg aagccgatcg tgccaagcag
     3721 gtggcgctga ttgccgcagc acaggatgca gaaaccaaag cggttgaact gaccgtgcgg
     3781 gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg cgatcgttga gttagccgaa
     3841 gctacgcgta aaaaaggcct ggcggaagca gaagcacaac gtgcgctgaa cgatgctatc
     3901 aacgtacttt ctgacgaaca aaccagcctt aaattcaaac tggccttgtt gcaggcgcta
     3961 cctgcggtaa tagagaaatc cgtggaacca atgaagtcca ttgacggtat caagattatt
     4021 caggtcgatg gtctgaatcg tggtggtact gcgggggatg caaacacggg taatgtgggg
     4081 ggcggaaacc tggcggaaca agcattatca gccgcactct cttaccgcac acaggcacca
     4141 ctgattgact ccttgctcaa tgaaattggc gtttcaggcg gctcactggc tgcattgact
     4201 tcactcttaa ccccaacaac tcccgtcgcc gaaaacgtag aataaatttt cagctatgaa
     4261 catccctggc tcaaaaagcc ggggattttt tatatctgcg ttccgctaaa aggtgcaaat
     4321 gatcaggctg ttgcagcgtt ttgcgtgatt gctcggggaa ggcaaaactg cttcagggaa
     4381 agcgttgcgc ggggtccggc gctcatcaac aatcggggag taagcaaagg gctgaaacgg
     4441 gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga agctcgtcat
     4501 catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa tcagaggggg
     4561 gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc agcgtcaaga
     4621 aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata tctgcgttcc
     4681 gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc ggggaaggca
     4741 aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag
     4801 caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttatagaag gaaagggtta
     4861 tgatgaagct cgtcatcata ctggttgtgt tgttactgtt aagtttcccg acttactaac
     4921 aactcatcag aggggggaga aatcctccct tatccttgtt cctttactct aggttgaaaa
     4981 gacaacagcg tcaataggcc tgccatgtac gaagcgagat ctgtgaaccg ctttccggtt
     5041 agcctttttt atcctgttgg atcttcttga taatgttagt ggtcgagcaa ccgtcttcaa
     5101 agttgagcac caacacttcg ccgccgttgg cccagacttc tttactcccg gcgatctctt
     5161 ccggtttgta gtcaccgcct ttcaccagca ggtctggcag gatcccggcg atcagacgct
     5221 gcggcgtgtc ttcttcaaac gacactaccc agtcgaccgc ttccagtgcg cccagcacaa
     5281 tcatacgctg ttcgagtggg tttaccgggc gggaatcccc tttcagccgt ttggtggagg
     5341 catcgctgtt gacggcaaca atcaagcggt cacccagctt gcgggcattt gccagataag
     5401 agacgtgccc ggcatgtagg atatcgaaga caccgttggt cattaccact ttttcaccac
     5461 gtttacgcgc tgccactaca gccagcttca gttcctcttc ggtcatcacg ccaaagccgg
     5521 tatctgcgcg tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg gacgtcccca
     5581 gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct tccagcgaat
     5641 tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt
     5701 catacacttc ctgcgcctga gttggcatat gcagcggcgc tttacccggt tgcagcagcg
     5761 acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt
     5821 tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca gcttcaaatt
     5881 ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg
     5941 gatcaatcag caccggaaca cccgctttac gcgccagttg gatcatctgc tgtacgcttg
     6001 ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc agcgcctgat
     6061 taatccgctc gtgcagcggc tgcggatcaa caccttcgaa accttcttca aaatccagac
     6121 ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt aatggtcgga tgcgtcggta
     6181 cagaaacgaa gtcgcatttg acgttgacgt cggccagaga tttactcagc gcgcgcgctg
     6241 catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca
     6301 tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca
     6361 ccggcgcttc cggcgagata cgactggagg ggccgtacca gtaacgatcc agcatcacat
     6421 caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct
     6481 cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt
     6541 ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg gcacatggcc
     6601 cggcaattcc tgtaatgcca gatgatgaag ttcatcgcga agcgtagtgt aagcacgggt
     6661 cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt ccagaatgcg
     6721 cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca acaccagata
     6781 ttgggtaata aattcgatat cggtaattcc cccttcatca gctttgatat caaagcgatc
     6841 gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca cttccgtttg
     6901 cagagtttta ccttcacgcg gcagcgtcat aatctcgcga cgcactgcgt caaagtgcgc
     6961 ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat gctcccacgt
     7021 ccaggcctcg tttttctgat aatcggcaaa tgcttctgtg gaggtaacca gcattcccgc
     7081 cgccccggac gggcgcagcc gcgcatccac ttcgtacaaa atgccggaag aggttcgcgt
     7141 gctgaacagg tgcataatgc gttgcgccag acgcagataa aactgtcgtc cgtcgatttc
     7201 ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca ggtcaagatc
     7261 ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccactg caaaaccacg
     7321 cccttcgcgt tcgttcaggt gattcggctt accgtagcgg gcaaccattt gaacccacgc
     7381 ctgctgaacg acggcatcta tcatggcttc cgccagccag gttaagtgat cgctcacttt
     7441 catcaccggt agcgtaccgg cgatatccgc tgcggcgatg cgtaacagct gcgcctgttt
     7501 gaactgacgc agcgcctcaa gctgttgctc ttcgtcatct tccggcacgc gcagcaaata
     7561 ctggcgcaac tcatcgcggt aggcatcggt cgccgtcggc tggtaaaggg tgtttggatc
     7621 gagcaattca tccagcaata atggataacg cgccagctgg ctggcaatca tcggcgacgc
     7681 tgcacacaga gaaatcaaat gtttaagcgc cgcggggaat tcactgagca attctaaata
     7741 ggtggtgcgg gtaacaatcc ccgccagcaa ggcggtaatg cgcaacagcg taacggcagc
     7801 gtcttcacgc gcacagacat cacttagcag atgcggcatc agatggtcga gcacctgacg
     7861 cccgcgtggc ccgattgtgc gcttatccag ctctttgcgg aaatcggcaa tcagcgtcag
     7921 cacctgtttg cgatcgtcct cgctaagatg cgccagcact ggtgtagtgt catcttcctg
     7981 caacgcatcc tgccacagct cgcgccactg ttccgacagc gactcttcct gagtttcact
     8041 ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggtcatat gtgcggtcag
     8101 cgccccggtc aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt
     8161 aagctcatca gaaggaagcg tctgggtttg ttcgtcgtta atgctttgca gcaggttttc
     8221 cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt cagaaagcag
     8281 atgcagcgcg gcaatgacgc tgagcgttgg cagtaaagag cgcgactgca gcgacggttc
     8341 gcgcccgccg cgaatgagct ggaacacctg aacgataaat tcaatttcgc gaatgccgcc
     8401 tgcgccgagt ttgatattgt cggtcaaacc acgtcgacgc acttcacggg caatcattcc
     8461 tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg
     8521 gcgcagcatc gcacgcaact cgttagcata gacgccttcg ctatcgccca taatccgcgc
     8581 cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat cttccaacgc
     8641 ggcaaagctc agcaccagcg ggccactttc gccaaacgga cgcagccgca tatccacgcg
     8701 atagacgaag ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg
     8761 ggtaaaaaac tgcgcgttat ccagttcccg gcgtccaccc tgcgtacaac catgttccgg
     8821 ccaggcaaaa atcagatcga tatcagagga gaaattcagt tccccgccgc ccagcttacc
     8881 catgcctaaa atcagcagcg gttgcgcttc gtcctgcgca ttgcacggcg ttccccactc
     8941 gcggcagcag gcgtcatata gccagtcacg cgccgcaaca atcagcgtct ccgccagata
     9001 gctgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg cccaggcgat
     9061 acgtaccatg atgcgccgac ggaacagccg cagctcgcgc attaacccgg cttcgtcact
     9121 cacattaatg agcgcctcct gcaaccatgc aacgtaatgc tgccattcgt cggcctgcgg
     9181 cggttggctt tccagttccg tcagccactc tggatgcgca cttatgctgt cctgcacaaa
     9241 atcactgaaa gtaagtactg acttcgcctg tgcactaagt gattcctcgg ctaaaggctc
     9301 tggcagccgc tcaacaatgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat
     9361 aagcgatttt atccttgcct gattaacgtt ttccgctgtg caaccagaac ggttcctgat
     9421 tgtttgcctc attacggaaa tgttcaattt cgatgcgttg cccggtcgca atagcgtgat
     9481 gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcatag taacccgcca
     9541 gcaacagtat tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg tagcgatcgc
     9601 ctaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga tggatatcgg
     9661 caaagcgttt gaaggagtcg ctgattttgc cctgcgcttt ggcatctaaa aatggctgcc
     9721 atgctttgct taccaaccat tcggtcaacg ccagcttcgc cattgccgtt tcggtagagt
     9781 agaccgccgt cacggcagaa accgcagaag caatggtcgc ttcgcattgg gtcagcaaat
     9841 cacgtaagtg agtgctcgct ttacgcggca caataccacc gaacagcatc agcgtatgac
     9901 ggaccaagct aatggctgcc agcacctgtt ctttcgccgc atcgttgccg cgtacccaca
     9961 gttcttcatg atactgccat tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt
    10021 ccacatcggc ttttgccgca acatgcaaaa tggtggtcgg tttgatttca cgcgccggat
    10081 tgccctgcgc cagatgatag ccacgcgccg ctttgctcag gctgccctgg cgtaatccgg
    10141 tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagct
    10201 ccagttccag ctcacagata ggttcagcaa attcccccgc tttcacctca ccctgatcga
    10261 gagcgatttc aattcggctg ccatcgaccg ccaccagcca tttttcgcga taaaaatcgg
    10321 tgctgaacag cggttgtacg cgggaagcga gatcggcggg caattcgccg ttcggccaga
    10381 cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg
    10441 ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc tcatagcgac
    10501 cgttttcgcc acgaatacgt aagcccatat cgtgcccacg cagccagtta tccggcgttt
    10561 cgtagtaaat attcagtaac tgcacggggt catggtgctc gccgcccagc gtattgagat
    10621 ggtcacgcaa cgcctcaacg gcgctgtgat taacaataaa ctttaattcg atttcctgag
    10681 ccatggcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga acttaatgcg
    10741 atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg
    10801 ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt gatgtcgaat
    10861 gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac aataacgaca
    10921 gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc gcgactgccg
    10981 tctcacacgc tgaagaaacg cgctatgttt ctgacgaact gaatacctgg gtccgtagcg
    11041 gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat
    11101 tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct
    11161 ggatcccgtt gaaacaactt agcactgagc caagcctgcg ctcccgtgtg ccagatctgg
    11221 aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc tggaatcagc
    11281 gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac gggttaaaag
    11341 aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca
    11401 gcgtgcagct ggatgacaaa cagcgcacca tcatcatgca atggtttatg tatggcggcg
    11461 gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc gcacctgatc ccaagccgca
    11521 aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg
    11581 aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc
    11641 gggatgcatt gttagggcta ccggtcaaag acagagattg ggtagtggtc ggcagtacgc
    11701 cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc
    11761 atccgcaaac gcatgaagag tatgcgctgg cacgtaccga acggaaatcc ggctccggtt
    11821 acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc
    11881 gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca
    11941 acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag
    12001 atccgttacg cgtactgcgc gtggcgcgtt ttgctgcgcg ttatgcccac ctcggttttc
    12061 gtattgccga tgaaactctg acgctgatgc gcgagatgac ccatgcgggt gaactggaac
    12121 acctgacgcc tgaacgggta tggaaagaga cagaaaacgc cctcaccacc cgcaatccac
    12181 aggtgttctt ccaggtactg cgcgattgcg gcgcactgcg cgttttattc ccggaaattg
    12241 acgccctgtt tggtgttccg gcccctgcca ggtggcatcc ggaaatcgac acgggtattc
    12301 ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat gtccgtttca
    12361 caactttatg ccacgatctc ggtaaaggac tgacgccgcc agaactctgg ccgcgtcatc
    12421 atggtcatgg cccggcgggt gttaagttag tggaacaact atgccagcgt ctgcgcgtgc
    12481 caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc atccacacct
    12541 tcccaatgct gaacccaaaa accatcgtca aattatttga ttccatcgat gcctggcgta
    12601 aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc ggcagaaccg
    12661 gttttgaatc agcggactac ccgcaaggcc gctggttgcg cgaagcctgg gaagtggcgc
    12721 agtcagtgcc gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag attcgcgagg
    12781 agctgacccg acggcggatt gcggcggtag ccagctggaa ggaacaacgt tgcccaaagc
    12841 ctgagtgagt attgggcatt ttgccggatg cggcgtaaac gccttatccg gctacatgtc
    12901 aacgacagtt gtaggcctga taagacgcgc aagcgtcgca tcaggcgttg attgccggac
    12961 gcggcgtgaa cgccttattc ggcctacgca atcgtggaca acgccaggca tccgacacta
    13021 ttcctcaatt aaaagaacac gacatacacc gcagccgcca caataaagcg ataaatggcg
    13081 aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc
    13141 accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccgct tgtcaggaag
    13201 ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggcac cgccagcagg
    13261 aacgaaaact cggaagcagc gtaacggctc acccccatca gcatcccacc tgaaatggtc
    13321 gccccggaac gggagaaacc cggccacagc gccagacact ggaaacaacc aatcataaac
    13381 gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat
    13441 tcggcggcaa tcagcaacaa accgccaacg accagcgcat acatcacatt tatcgggtta
    13501 aacaatgact taatcgtgtc gtggaacaac agccccagta ccaccgccgg aatcatcccc
    13561 agcaaaatgt ggatcagcgt taaacgacct ttgctttctc cttcgtgctg caacgggcgg
    13621 ccaaagtgga tgccaatcag gccaaacaga cgccgccaga acatcactac taccgccaga
    13681 attgatccta actggatcac aacttcaaag gttttcgccg tgtcgccctc aaaccccaac
    13741 aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc tgtcaatcct
    13801 tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat caataaaccc
    13861 ctaaattata aaaatgtaac gcttgtccga actactgcag tatgaccagg ttataaccgt
    13921 ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg ctcaatgatt
    13981 acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat acgcacccac
    14041 ggcgagttga agcgtgctaa cagcagctct gccacctctt ccgccacgcg ctccaccagc
    14101 gcaaaacgtg ccccctcgac gtggctgacc accgtttctg caatgtcagc gtagctgagg
    14161 caatccgcca cattatcgct ttttgccgct ttacggttat cccacgccat ttcgatatcg
    14221 aacactaatt tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc
    14281 gaaagttgct ctataaatac aatatccatc acgacctgct cgctttttgg ctaacccgga
    14341 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgtttaaca cgaattttca
    14401 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    14461 gctccatttc cagtgccatt ctggtttgcc gcctgtgtgg gctgcccgat ccgcgaacca
    14521 gcggctccgg taatcctggc gcaactaacg tgttacgtat aggtggcaaa ggagcagccg
    14581 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    14641 taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    14701 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    14761 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    14821 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    14881 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    14941 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    15001 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    15061 ccttcatcgg gtttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    15121 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    15181 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    15241 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcagcacaat ctcgccgcta
    15301 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    15361 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    15421 tgtagggatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    15481 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    15541 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatacacc
    15601 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    15661 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg
    15721 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    15781 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    15841 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag cgtcgccgct
    15901 gcagcactaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    15961 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    16021 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    16081 ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    16141 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    16201 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    16261 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    16321 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    16381 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    16441 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcgaccat
    16501 caaagcgccg ctgcgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    16561 taccggtagc ggcgtaccgt gggtcacctg ggatatcgtc cccgacggtg acgatgcgga
    16621 aatcgaagtt tacatggcag gcggcggctg cacgctacca ggccgctcga aagtgttaat
    16681 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    16741 agtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    16801 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccacc ccaatccaaa
    16861 agcggcagag ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    16921 agggctgact ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg cccgccatcc
    16981 gtcaaccatc ggcgttgctg tttctaccgg ttgctgggcg catcgtcgcg gcacactgct
    17041 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    17101 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcacgtcgg cgatgtgatc
    17161 tatctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgatt gatcgaactg
    17221 aaacgtccgc tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    17281 cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggtc cgacaaccag tatgcgtatg
    17341 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt cggcaaaggc
    17401 ggaatggggc cgctgaccga agaaggctgt cagaaattca aggcgctaca tgtaattttc
    17461 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    17521 acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg
    17581 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    17641 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    17701 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    17761 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    17821 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtaatcgtt
    17881 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat ctccatcatc
    17941 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    18001 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    18061 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggctggg gcgacgtatc
    18121 gcgctgattc tggtgaaaaa gatggggcat cgcacgctgt ttcttggcta tgcggtgatg
    18181 ttctccgagc tgatcctggc acctgtcaca ccgtccaact cggcgcgtgg tgcggggatt
    18241 atctatccca tcatccgtaa cctgccgccg ctctatcaat cacaaccaaa cgacagcagt
    18301 tcgcgcagta ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    18361 gccattttcc tgacggcgat ggctcctaac ttgctgttaa ttggactgat gaaaagcgca
    18421 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtatttta
    18481 ctggtcctgc tggttccctg gctggcttac gtgctgtacc cgcctgtact gaagtcaggc
    18541 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    18601 gaaaaacgga tgctggggct gatggtaggc gcgctggtgc tgtggatttt cggcggtgat
    18661 tatatcgatg ccgcgatggt cggttacagc gtggtggcgc tgatgctgct tctgcgcatt
    18721 atcagttggg acgatattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    18781 tcgcttatca ccctcgcgac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    18841 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    18901 gtgttttatc tactgcgcta cttttttgcc agcgccacgg cgtatacctc cgctctcgca
    18961 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    19021 atggttggtg cggcaattgg tctggggagc attcttacac catacgccac cggccccagt
    19081 ccgatttact acggcagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    19141 tttgggctga tattcctcgt attgctggtg attaccggct tactgtggat gcccgtggtg
    19201 ttgctttaag taaggacggt gccggaagta actctccggc acccgatagt tttacgcagc
    19261 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    19321 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaattc
    19381 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    19441 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    19501 cagcgcacgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    19561 gatgtcagca cgcgtctggt cgtcggtgcc gttgtcacga atggtatttg ccgcgaaggt
    19621 tttcaggccg gagaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    19681 aaagcgcccg gcagtaccct gcgccgccat tttcgacagt aacggtccgc caggataatc
    19741 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    19801 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    19861 aaccagcagc gcaacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    19921 atgcccttcc atatggtgta cagggatcgc cggaacgttc caggcaaacg ccagagaacg
    19981 ccccacggtc gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    20041 agcatcaatg tcttttgccg ttaaaccaga ctcctttagc gccgcctgga tcaacggtac
    20101 ggttttacgc acatgatcgc gggaggccag ttcaggcaca acgcccccgt agtcagcgtg
    20161 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    20221 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    20281 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    20341 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt
    20401 gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat aagctggcgt
    20461 tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt aattaaagta
    20521 cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca
    20581 ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag
    20641 cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc
    20701 cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg agttgtagtt
    20761 gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat
    20821 cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg gcacgcactg
    20881 acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag aatttccacg
    20941 cgtgttgtcc attccacaac gagaaaaccc cgtccttcac cgttaacggt gagaaacagt
    21001 tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg
    21061 acaagcttga gttcgtcgaa acggtcgaag agctggcggc aatgcacaat cttgaagtgc
    21121 catttgaagc aggtagcggc cccagccaga tcgagcgcca tcagcggcaa acgctttatc
    21181 agttgatgga cggtctgaat acgttttacc aacaatcttt acaacaacct gttgccacgt
    21241 ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgct cgctttgcga
    21301 ttggttttgc gcctcccggc tgggacaacg tcctgaagcg gtttggcggc aatccagaaa
    21361 atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcaggga cgcagttacg
    21421 atcgtttccg cgagcgggtg atgttcccca ttcgcgataa acgcggtcgg gtgattggtt
    21481 ttggcgggcg tgtgctgggc aacgataccc ccaaatacct gaactcgccg gaaacagaca
    21541 ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat aacgctgaac
    21601 ccaatcgtct gcttgtggtc gaaggctata tggacgtggt ggcgctggcg caatacggca
    21661 ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata caactgttgt
    21721 tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc cgtgatgccg
    21781 catggcgagc gctggaaacg gcgctgcctt acatgacaga cggccgtcag ctacgcttta
    21841 tgtttttgcc tgatggcgaa gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt
    21901 ttgaagcgcg gatggagcag gcgatgccgc tttccgcatt tctgtttaac agcctgatgc
    21961 cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg gcactgccat
    22021 tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca
    22081 aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg gcagagagcg
    22141 gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt atagggttgc
    22201 tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg gatgaaaata
    22261 agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc cagccaggtc
    22321 tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc acccttgaaa
    22381 aactgtcgat gtgggacgat atagcagata agaatattgc tgagcaaacc ttcaccgact
    22441 cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg
    22501 agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta aaccaggagc
    22561 tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc
    22621 cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt tggcggtaaa
    22681 caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc caacaccaac
    22741 ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac agctgaaact
    22801 tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca atgaccatct
    22861 gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga tcaacgacat
    22921 gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac
    22981 cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat
    23041 cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt
    23101 gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca accaggttca
    23161 atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt acgatcgtgt
    23221 tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc cgaacgcaga
    23281 agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg aagatctgga
    23341 cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg atgacaacag
    23401 catcgacccg gaactggctc gcgaaaaatt tgcggaactg cgcgctcagt acgttgtaac
    23461 gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa
    23521 actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact acctggtcaa
    23581 cagtatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca tgaagctctg
    23641 cgttgagcag tgcaaaatgc cgaagaaaaa cttcattact ctgtttaccg gcaacgaaac
    23701 cagcgatacc tggttcaacg cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca
    23761 cgatgtctct gaagaagtgc atcgcgccct gcagaaactg cagcagattg aagaagaaac
    23821 cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa
    23881 agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta tttctatcgc
    23941 caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag gcaacatcgg
    24001 tctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct ccacctacgc
    24061 aacctggtgg atccgtcagg cgatcacccg ctctatcgcg gatcaggcgc gcaccatccg
    24121 tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc gccagatgct
    24181 gcaagagatg ggccgtgaac cgacgccgga agaactggct gaacgtatgc tgatgccgga
    24241 agacaagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg aaacgccgat
    24301 cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc tcgagctgcc
    24361 gctggattct gcgaccaccg aaagcctgcg tgcggcaaca cacgacgtgc tggctggcct
    24421 gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt atcgatatga acaccgatca
    24481 cacgctggaa gaagtgggta aacagttcga cgttacccgt gaacgtatcc gtcagatcga
    24541 agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgctgc gtagcttcct
    24601 ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacct gtccctttgc
    24661 acaaacgcca ccttttcggt ggcgtttttt atcgcccacg caccaccagc gcctggtcca
    24721 gctcgcgata cgcttcaacc agtttctcca gtgaaacgcg acttaaaccg ctgggatttg
    24781 gcagcaccca aatctgcgtc gaaccaatgc tgagcgtttg tttcccccac tgtgcaccac
    24841 gctggctgaa tccctgttca tatgcttgtt tgcccagaat cgccaacgct tgcggctgat
    24901 aatcttcaat tttttcaatc agcttacgcc cacctgcgtg tagctcttgc tttgaaattt
    24961 cattggcttg caccgttggc ctgtctacca gtttggtgac gccacaacga taatccagca
    25021 gatgctgtgc ctcctgcggc ttcaactgat ggtcggtaaa cccggcctga tatatcacct
    25081 tccagaagcg atttgccgga tgagcaaagg gaaaaccagt cccggcggat gaaagcccag
    25141 ggttgatacc gcaaaacacg acccgtaacc ctggagccaa aatatcctca accatattta
    25201 ctcctttcta acatcaccag gaaaagtata acggattgaa aatacattgt ttataaaaac
    25261 agcaggcgcg cggtaatggc tggattgcga cacggagtta ctttataatc cgctaccatg
    25321 gccccttagc tcagtggtta gagcaggcga ctcataatcg cttggtcgct ggttcaagtc
    25381 cagcaggggc caccagatat agcaaaggct gacgagaaat cgtcagcctt tttcttttta
    25441 tatatcagtt actttgcgtg ccagtaggcc gctgcacgta cccgctgtgg gtcatactgt
    25501 tccgcttcaa agcggcggct taaattctta acgactttac cttcgccggt tatccagatg
    25561 aagtaatcat cggcagggat ttgcatctgc gccagacgcg catctaccgc ctgctcatca
    25621 tgtgccagcc attcgatatt aaaaccatca aggtgcgcga gataatcctg acaggcgtta
    25681 tcccgcacgc taaccagcgc actaacttgc ggtttaacgg caagtttgct caacgtttcc
    25741 aggcggcggc gcaatgcggg cattccggat tcatcgcaga catacagctg atacgcgtaa
    25801 tcttccggca ccaccagcga accgcgcgga cctgccaccg taagtttatc gcccggttgc
    25861 gcctgcatcg cccagccgct ggcgacccca ccgtcgtgaa taaagaaatc gatcgccagt
    25921 tcatggcgta gttcgtcata cagcggcgta tagtcacgcg acggtgggcg tggtccttcc
    25981 ggccagacga tgccctcttc cgttaccgtt ggcggcacaa agtgagcgtc aggttgagga
    26041 aagaagagtt tgctgtgatc gtcaaagccg cgcgatgtaa aaccgtccag cgcctcgccg
    26101 ccaaggacaa tgcgctgaaa accggcgctg atacgctcaa cgcgtagcac agtcaattca
    26161 cggaagcgca gatcattgcg aacgcgctgc gggtagcggg gggagttatt cattgttatc
    26221 gccttcgtga tggtaatcag atatatctaa ataattttcg caaatgataa tgattgttaa
    26281 tcatgataaa tgcaagcgat ttgtagaact gatatgtcta tagtctgaca agacgaaccg
    26341 cctcttctca ggcatcatta ctcaacgccg gatgcggcgt gaacgcctta tccggcctac
    26401 gtgtgagatg agtccacatt taaaaatcat ttttattctt gcaagagtgc tcacatcaga
    26461 tataaattag atatatctaa ttaagcaaaa ggaggctgat atgagccatc atcacgaagg
    26521 gtgttgtaaa catgaaggcc agccacgcca tgagggctgc tgcaaaggtg agaagtcaga
    26581 acacgagcac tgcggacacg gtcaccagca tgaacacggt caatgttgcg gtggtcgcca
    26641 cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac ggtgaattgc gtctggtgat
    26701 tctggatatt ctctcgcgcg atgacagcca cggttacgaa ttgattaaag cgattgagaa
    26761 tctaacccaa gggaattaca ccccaagccc gggcgtcatc tacccgacgc tggattttct
    26821 gcaggagcag tcgctgatta ccatccgcga agaggaaggt ggtaagaagc agattgcgct
    26881 gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa caggtggaga tgattgaaga
    26941 acgcatcaaa gcgcgttgcg ttggcgctgc gctgcgccag aacccgcaaa tgaagcgggc
    27001 gctagataat tttaaagcgg tgctgaattt acgcgtcaac cagagtgata tcactgatgc
    27061 acaaataaaa aagatcattg cggtgatcga ccgcgccgct tttgatatta cgcaactgga
    27121 ttaatcgccg catccgccag tggcgcggtg caattgccgg atgcgacgct tgacgcacct
    27181 tatccggcct acacccgcta cacaccccgt aggcctgata agatgcgcca gcatcgcatc
    27241 aggcatcgtg tatcaaatac cggatccggc ataccgatta atgcagcacc gtcaccgcgt
    27301 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
    27361 gctcggcatt tttctgggtg atgaggttaa gctcatccac tgcacgggtc aggctggaaa
    27421 gaccatcggc ctgttccagc gttgaatggc taatctgggc aatcaactgg gtgacgtttt
    27481 tcacctgtgc cacaatatct tccatcgtac gtccggcggc gtgtacctgc tgcgaaccgg
    27541 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattggcgc
    27601 tgcggctggc taaatgacgc acttcccccg ccaccaccgc aaaacctttg ccctgttcgc
    27661 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
    27721 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
    27781 tcttgatcac cgtggtcatc gcctccccgc cctgcactgc cgcattactg gcggtgattg
    27841 acagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
    27901 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
    27961 catcggtgcc tttcgccagc gtctcactgc cgtttctgac actggacacc tggcttgaga
    28021 catcgttaat taaccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
    28081 ccagctcatc gctgcgattc agatgctcaa cactattacg ctctccggtc gccaccttca
    28141 gtgcctgacg ggcgacattt tctatcgggc ggacaatctg ccattcaaaa caggcgcttg
    28201 ccaacaatac cactaacgca cagaggatat acgtcaccac cggggcagca acaaaccaaa
    28261 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
    28321 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
    28381 taccggtacg tccggcgttc agcgctttgt acagcggctc caccgccgcg atctcttcat
    28441 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
    28501 ccgcattggc ccgcacccaa taatggtcac catttttgcg gcgatttttc acgatgccgc
    28561 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
    28621 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
    28681 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
    28741 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
    28801 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
    28861 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
    28921 ctaattcgtt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
    28981 cccaaaaatc aggcagttaa tgccctgaaa gcgtgcattt gcgcagcaat tatcaaatcc
    29041 atacccgaca aaaaccgtgc aaaataacaa cagatgttaa caaatagcat taaatattgc
    29101 gcaaatgata accacatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
    29161 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
    29221 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
    29281 aaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
    29341 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
    29401 ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca cccagggaca
    29461 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
    29521 tgtggtttcc gccgtacaga atcaacttgc gaaacaacca ctgcacagcc aggagctact
    29581 cgatccgtta cgggctatgc tggcgaaaac ccttgctgcg ctaacgccag gtaaactgaa
    29641 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcggcgctga agctggcgaa
    29701 ggcttatcag tcgccgcgcg gcaaatttac ttttattgcc accagcggcg cgttccacgg
    29761 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
    29821 actgctgcca gggttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
    29881 taatgagtgc aaaaaaaccg gtgatgatgt ggccgcggtg atcctcgaac cgattcaggg
    29941 cgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtgcgta agctgtgcga
    30001 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
    30061 gatgttcgcc tgcgaacatg agaacgtaca gccggacatc ctgtgcctgg caaaagccct
    30121 cggcggcggt gtgatgccga ttggcgcaac catcgccact gaagaggtgt tctcagttct
    30181 gttcgacaac ccgttcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
    30241 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgagcaaaa
    30301 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
    30361 ggaagcgcgt ggtaaaggga tgttaatggc gattgagttt gttgataacg aaatcggcta
    30421 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
    30481 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactcgtgat
    30541 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtagtatca
    30601 aatcggatgg caatacgacg tcgccatccg atctttttat acgatacgta cgcccgcagg
    30661 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
    30721 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
    30781 caccgttttt cccatcaact cttcttcgct gtagtacggc accaggctgg tcacggtttg
    30841 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
    30901 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
    30961 ggtttccatt gcttcctccc ttcgagtaaa attttactaa actatagcaa aagtttttct
    31021 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtgag ccgctgtgtt
    31081 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
    31141 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacactaa
    31201 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
    31261 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
    31321 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
    31381 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
    31441 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
    31501 aacacagcgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
    31561 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
    31621 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
    31681 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
    31741 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
    31801 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
    31861 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
    31921 ttgctatcgg cgtactgcgg gcgattcatg aacgtggtct gaacatccca caggatattt
    31981 cgcttatcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
    32041 tgcgcatcca ttccgaaatg atgggaagtc agggcgtaaa cctggtgtat gaaaaagccc
    32101 gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa ctgcgcggca
    32161 cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccgggc agtgctgccg
    32221 tttattttcg tgatccagtt aaagtaaatg catttacccg ctacttttta gtaaaaattt
    32281 tactaaactc cccagcaatt acacaaacta ccatcaccat gaatggttcc gatttctctc
    32341 taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa aaccgacttg
    32401 cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc tttgcccgcg
    32461 aaacgagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt tttgaccatc
    32521 cgctgcaagt gccagaagcc ttcacctctg agttaatggc tgactggggg catattaccg
    32581 tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac gaaggttttc
    32641 cgttccccat cgatgtgccg tttgttccca gcgataaccc aaccggtgcc tatcaacgta
    32701 ttttcaccct cagtgacggc tggcagggta aacagacgct gattaaattt gacggcgtcg
    32761 aaacctattt tgaagtctac gttaacggtc agtatgtggg tttcagcaag ggcagtcgcc
    32821 tgaccgcaga gtttgacatc agcgcgatgg ttaaaaccgg cgacaacctg ttgtgtgtgc
    32881 gcgtgatgca gtgggcggac tctacctacg tggaagacca ggatatgtgg tggtcagcgg
    32941 ggatcttccg cgatgtttat ctggtcggaa aacacctaac gcatattaac gatttcactg
    33001 tgcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa gtggtgctgg
    33061 aaaatctcgc cgcctcccct gtcgtcacga cgctggaata taccctgttt gatggcgaac
    33121 gcgtggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc agcgccagct
    33181 ttgcttttac tgtcgaacag ccgcagcaat ggtcagcaga atccccttat ctttaccatc
    33241 tggtcatgac gctgaaagac gccaacggca acgttctgga agtggtgcca caacgcgttg
    33301 gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt tatgtgatgc
    33361 tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt ggaatggatc
    33421 gcgtcgagaa agatctccag ttgatgaagc agcacaacat caactccgtg cgtaccgctc
    33481 actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcctg tttgtgatgg
    33541 cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc cgtattaccg
    33601 acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt cacgcgcaga
    33661 aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat ggctgtaaca
    33721 tccgcgcgat gtaccacgca gcgaaggcgc tggatgacac gcgactggtg cattacgaag
    33781 aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc gtgccgctga
    33841 tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa tatgctcatg
    33901 cgatggggaa cggaccgggc gggctgacgg agtaccagaa cgtcttctat aagcacgatt
    33961 gtattcaggg acattatgtt tgggagtggt gcgaccacgg gatccaggcg caggatgaca
    34021 acggcaatgt ctggtataaa ttcggcggcg actacggcga ctatcccaac aactataact
    34081 tctgtcttga tggtttgatc tattccgatc agacgccggg accaggcctg aaagagtaca
    34141 aacaggttat cgcgccggta aaaatccacg cgctggatct gactcgcggc gagctgaaag
    34201 tcgaaaataa actgtggttt accacgctgg atgactacac cctgcacgca gaggtgcgcg
    34261 ccgaaggtga aacgctcgcg acgcaacaga ttaaactgcg cgacgttgcg ccgaacagcg
    34321 aagccccctt gcagatcacg ctgccgcagc tggacgcccg cgaagcgttc ctcaacatta
    34381 cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc gccacttatc
    34441 agttcccgct gaaggaaaac accgcgcagc ctgtaccttt cgcaccgaat aacgcccgtc
    34501 cgctgacgct ggaagacgat cgtttgagct gtactgttcg cggctacaac tttgcgatca
    34561 ccttctcaaa aacgagtggt aaaccgacat cctggcaggt aaatggcgaa tcgctgctga
    34621 cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag caggagtacg
    34681 aagggttgtg gcagccgaat catttgcaga tcatgcagga acatctgcgc gactttgccg
    34741 tagaacagag cgatggtgaa gtgttgatca tcagccgcac ggttatagca ccgccggtgt
    34801 ttgacttcgg gatgcgctgt acctacatct ggcgcatcgc tgcagatggc caggttaacg
    34861 tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc atcggtttca
    34921 ccatggggat taacggcgaa tacgatcagg tggcgtatta cggtcgtgga ccgggcgaaa
    34981 actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc gtcgatgcca
    35041 tattcgagaa ctatcccttc ccgcagaaca acggcaaccg tcagcatgtc cgctggacgg
    35101 cactgactaa ccgccacggc aacggtctgc tggtggttcc gcagcgccca attaacttca
    35161 gcgcctggca ctatacccag gaaaacatcc acgctgccca gcactgtaac gagctgcagc
    35221 gcagtgatga catcaccctg aatctcgacc accagctgct tggcctcggc tccaattcct
    35281 ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc tacggcttta
    35341 cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag cctggcgtcg tatgagttcg
    35401 gcgcagggtt cttttccaca aatttgcaca gcgagaataa gcaatgagga tcatcgataa
    35461 cttagaacag ttccgccaga tttacgcctc tggtaagaag tggcaacgct gcgttgaagc
    35521 gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg actcattgac
    35581 ttaccgcgtg gagacagact ccgcgaccga tgcgctattt accgggcatc gacgctattt
    35641 tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga aagagacatt
    35701 acaggtcgtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag gctgcggaga
    35761 aaccgttgag gtccacgaag ggcaaatcgt tatttgcgat atccatgaag catatcggtt
    35821 tatctgcaat aacgcggtca aaaaagtagt tctcaaagtc accatcgaag atggttattt
    35881 ccataacaaa taacaactac ggcggcaaaa ggagtttgcc gccaccgcta ccctactcat
    35941 tttcggagat gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt cggcttgctc
    36001 tcgctgactt ttgccgccgt ttacagcttt aacaacgtta tcaataataa tattgagctt
    36061 ggactggcct cggcaccgat gtttttcctc gcgacgattt tttattttat tcccttctgt
    36121 ctgatcatcg cagaatttgt ttcgttaaat aaaaactcag aagccggtgt ctacgcgtgg
    36181 gtaaaaagtt cgctgggcgg acgttgggca tttattactg cctataccta ctggttcgta
    36241 aacctgttct ttttcacctc gctgttgccg cgcgttattg cttatgcttc gtatgccttc
    36301 ctcggctatg aatatattat gacgccggtt gccaccacca ttatcagtat ggtgctgttc
    36361 gccttctcca cctgggtttc caccaacggg gcgaaaatgt tggggccaat tacctccgtc
    36421 acttcaacgc tgatgctgct gttaacgctc tcctacattt tactggcagg tacggcgctg
    36481 gttggcggcg tacagcctgc tgaccccatc accgttgacg cgatgatccc gaacttcaac
    36541 tgggcgttcc tcggcgttac cacctggatc tttatggccg caggtggcgc ggagtccgtc
    36601 gctgtgtacg ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt gatcatcctc
    36661 gccgggattt ttatcggtgt actatattcc gtctcctcgg tgctgattaa cgtcttcgtc
    36721 agcagcaaag agttgaaatt tactggcgga tcggtgcagg tattccacgg catggcggcg
    36781 tattttggtc tgccggaagc gttgatgaat cgctttgtcg gtctggtgtc ctttaccgca
    36841 atgttcggtt ccctgctgat gtggaccgca acgccggtga aaattttctt ctccgaaatc
    36901 ccggaaggca tctttggtaa gaaaaccgtc gaacttaacg aaaacggcgt tccggcgcgc
    36961 gcagcgtgga tccagttcct gatcgtcatc ccgctgatga ttatcccgat gctcggttcc
    37021 aatactgtgc aggatctgat gaatactatt attaatatga ccgccgcagc gtccatgctt
    37081 ccgccgttat tcatcatgct ggcttacctg aatttacgcg ccaaattaga tcacctgcca
    37141 cgcgatttcc gtatgggctc ccgccgcacc ggtattatcg ttgtttcaat gctgattgcg
    37201 atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat tctgaccatc
    37261 attttttata acgtcggcgg tattgttata ttccttggct ttgcgtggtg gaaatacagt
    37321 aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc agccagcaat
    37381 gttgattaaa cagactaata aaaaatgagc aggcattttc cctcttcctg gatgagg
//
LOCUS       DAZGXS010000030        36391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGXS010000030 DAZGXS010000000
VERSION     DAZGXS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1042)
                     /locus_tag="ACHKG5_001672"
     CDS             complement(<1..1042)
                     /locus_tag="ACHKG5_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0867794.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDEVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPDAGNNTTRIATTAFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFSTTVLNALAGKQPLDNT
                     LTNLSGKDVAGLL"
     gene            complement(1039..1650)
                     /locus_tag="ACHKG5_001673"
     CDS             complement(1039..1650)
                     /locus_tag="ACHKG5_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0867795.1"
                     /translation="MSDSRLLPTGSSPFEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDNWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1643..2551)
                     /locus_tag="ACHKG5_001674"
     CDS             complement(1643..2551)
                     /locus_tag="ACHKG5_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP0867796.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAAVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNNTQASFCTEYSVVTGGSDE"
     gene            complement(2556..2903)
                     /locus_tag="ACHKG5_001675"
     CDS             complement(2556..2903)
                     /locus_tag="ACHKG5_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0867797.1"
                     /translation="MTARYLGMNRSDGQTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2900..3535)
                     /locus_tag="ACHKG5_001676"
     CDS             complement(2900..3535)
                     /locus_tag="ACHKG5_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0867798.1"
                     /translation="MNTLANIQELARALRNMIRTGVIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3602..4054)
                     /locus_tag="ACHKG5_001677"
     CDS             complement(3602..4054)
                     /locus_tag="ACHKG5_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0867799.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQAAMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(4047..4514)
                     /locus_tag="ACHKG5_001678"
     CDS             complement(4047..4514)
                     /locus_tag="ACHKG5_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0867800.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4477..4722)
                     /gene="lysC"
                     /locus_tag="ACHKG5_001679"
     CDS             complement(4477..4722)
                     /gene="lysC"
                     /locus_tag="ACHKG5_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP0867801.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4622..5047)
                     /gene="lysB"
                     /locus_tag="ACHKG5_001680"
     CDS             complement(4622..5047)
                     /gene="lysB"
                     /locus_tag="ACHKG5_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP0867802.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     ATITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(5035..5460)
                     /locus_tag="ACHKG5_001681"
     CDS             complement(5035..5460)
                     /locus_tag="ACHKG5_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP0867803.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTALFLQPVPWRRRSVALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(5475..5972)
                     /locus_tag="ACHKG5_001682"
     CDS             complement(5475..5972)
                     /locus_tag="ACHKG5_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP0867804.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     QEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5972..6253)
                     /locus_tag="ACHKG5_001683"
     CDS             complement(5972..6253)
                     /locus_tag="ACHKG5_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0867805.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKSRGKP"
     gene            complement(6257..6460)
                     /locus_tag="ACHKG5_001684"
     CDS             complement(6257..6460)
                     /locus_tag="ACHKG5_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0867806.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6460..6969)
                     /locus_tag="ACHKG5_001685"
     CDS             complement(6460..6969)
                     /locus_tag="ACHKG5_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0867807.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(7069..7812)
                     /locus_tag="ACHKG5_001686"
     CDS             complement(7069..7812)
                     /locus_tag="ACHKG5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP0867808.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7816..8889)
                     /locus_tag="ACHKG5_001687"
     CDS             complement(7816..8889)
                     /locus_tag="ACHKG5_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0867809.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(8944..9798)
                     /locus_tag="ACHKG5_001688"
     CDS             complement(8944..9798)
                     /locus_tag="ACHKG5_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0867810.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMENAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            9972..11744
                     /locus_tag="ACHKG5_001689"
     CDS             9972..11744
                     /locus_tag="ACHKG5_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP0867811.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11744..12778
                     /locus_tag="ACHKG5_001690"
     CDS             11744..12778
                     /locus_tag="ACHKG5_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0867812.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFATGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDALQDR
                     DYIDSIRENMANSKGRNNFKNLFLYAPQGKPDGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            12991..13260
                     /locus_tag="ACHKG5_001691"
     CDS             12991..13260
                     /locus_tag="ACHKG5_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867813.1"
                     /translation="MQMHEHAEGAPVPAALTRDWEGKRMRFDAVMHAELKMAEHTEKT
                     HKKTGIQVAGLSRLHCGYWPRNAPIILVRQQVATISKKTVVHGFS"
     gene            complement(13291..13485)
                     /locus_tag="ACHKG5_001692"
     CDS             complement(13291..13485)
                     /locus_tag="ACHKG5_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867814.1"
                     /translation="MHNSLSSIKDPYADWAKRLTVMASNNDLSSREVESYTEKMVEQA
                     SKDELTVVIKHLLNHIRMNK"
     gene            13484..13921
                     /locus_tag="ACHKG5_001693"
     CDS             13484..13921
                     /locus_tag="ACHKG5_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867815.1"
                     /translation="MSKNLELFNQQTAEIFAVLWDNFPVPQVITYKKFNAALPDDYFD
                     QLNSPEMKALNQLRSVVDGTFTFLSENGYILYGTDHQTGFHDVRLTEKALAVLNKKPE
                     ALGGNETMGDKIISAVKDGTPGVIAGAVTNLLTLGVNFVTSAS"
     gene            complement(14046..14996)
                     /locus_tag="ACHKG5_001694"
     CDS             complement(14046..14996)
                     /locus_tag="ACHKG5_001694"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFP0867816.1"
                     /translation="MKKRKKLKVNTKNKSYDIMDSPFYKLKSKRKLANLLCVSLDDLY
                     SLRKDEGNYSVFEQLSKKGKARKIQKPLEKLELVHTRIASLLSRIALPEYLHSGKKKC
                     SNVTNAKAHLNNEKMMTTDIKAFFPSTTRGMIFSFFFSVMKMSSDVADVLSHICTCHD
                     RLPTGSRISMPLAYFANSRMFGEIYQLCQKLRVNMTVYVDDLTFSGSNVNRLFCAVIR
                     KIVNKHGHVIHPTKTKLYARDKPKLVTGVIVLGNALKVRNEQHFLMAREIDYWKIIKD
                     AENARETIIAKKLFGRLHSMGVIEQRYKSKVLTLKANTAN"
     gene            complement(14974..15282)
                     /locus_tag="ACHKG5_001695"
     CDS             complement(14974..15282)
                     /locus_tag="ACHKG5_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0867817.1"
                     /translation="MLHKALRLIRQYHKESIVELSSSLGIPKDKIVSLESGVWSPSIE
                     VLERYASHFDIPVSSLVFFSESLGTQGRLSKRLRLNLAGKVLDVLEWVSNKNEKTEKA
                     "
     gene            complement(15319..15474)
                     /locus_tag="ACHKG5_001696"
     CDS             complement(15319..15474)
                     /locus_tag="ACHKG5_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867818.1"
                     /translation="MKNDKLTNEQQAQNKSNLKKWAGYAWKLIKWGIRLFDLVTRALD
                     YFEGGDE"
     gene            complement(15883..18171)
                     /locus_tag="ACHKG5_001697"
     CDS             complement(15883..18171)
                     /locus_tag="ACHKG5_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0867819.1"
                     /translation="MAVKASGRFVPSSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEEICDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTTPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHDLRTPNRQ
                     QRSGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWEIEALARGATVNYDG
                     KKFTYMVINEWFGFRDEESFFM"
     gene            complement(18161..18436)
                     /locus_tag="ACHKG5_001698"
     CDS             complement(18161..18436)
                     /locus_tag="ACHKG5_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP0867820.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGTHGR"
     gene            complement(18433..18657)
                     /locus_tag="ACHKG5_001699"
     CDS             complement(18433..18657)
                     /locus_tag="ACHKG5_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFP0867821.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(18657..18959)
                     /locus_tag="ACHKG5_001700"
     CDS             complement(18657..18959)
                     /locus_tag="ACHKG5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP0867822.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLGLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADEFKKLNDKRNW"
     gene            complement(18959..19183)
                     /locus_tag="ACHKG5_001701"
     CDS             complement(18959..19183)
                     /locus_tag="ACHKG5_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP0867823.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(19247..19747)
                     /locus_tag="ACHKG5_001702"
     CDS             complement(19247..19747)
                     /locus_tag="ACHKG5_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFP0867824.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(19917..20189)
                     /locus_tag="ACHKG5_001703"
     CDS             complement(19917..20189)
                     /locus_tag="ACHKG5_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP0867825.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            20326..20619
                     /locus_tag="ACHKG5_001704"
     CDS             20326..20619
                     /locus_tag="ACHKG5_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0867826.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            20689..21669
                     /locus_tag="ACHKG5_001705"
     CDS             20689..21669
                     /locus_tag="ACHKG5_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0867827.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(21856..22356)
                     /gene="cpxP"
                     /locus_tag="ACHKG5_001706"
     CDS             complement(21856..22356)
                     /gene="cpxP"
                     /locus_tag="ACHKG5_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0867828.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22506..23204
                     /gene="cpxR"
                     /locus_tag="ACHKG5_001707"
     CDS             22506..23204
                     /gene="cpxR"
                     /locus_tag="ACHKG5_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0867829.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPRKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23201..24574
                     /gene="cpxA"
                     /locus_tag="ACHKG5_001708"
     CDS             23201..24574
                     /gene="cpxA"
                     /locus_tag="ACHKG5_001708"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0867830.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24680..25354)
                     /gene="yiiM"
                     /locus_tag="ACHKG5_001709"
     CDS             complement(24680..25354)
                     /gene="yiiM"
                     /locus_tag="ACHKG5_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0867831.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25503..26486)
                     /gene="kdgT"
                     /locus_tag="ACHKG5_001710"
     CDS             complement(25503..26486)
                     /gene="kdgT"
                     /locus_tag="ACHKG5_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0867832.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26746..27366)
                     /gene="sodA"
                     /locus_tag="ACHKG5_001711"
     CDS             complement(26746..27366)
                     /gene="sodA"
                     /locus_tag="ACHKG5_001711"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0867833.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27651..28685
                     /gene="rhaT"
                     /locus_tag="ACHKG5_001712"
                     /pseudo
     CDS             27651..28685
                     /gene="rhaT"
                     /locus_tag="ACHKG5_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(28682..29530)
                     /gene="rhaR"
                     /locus_tag="ACHKG5_001713"
     CDS             complement(28682..29530)
                     /gene="rhaR"
                     /locus_tag="ACHKG5_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0867834.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29604..30440)
                     /gene="rhaS"
                     /locus_tag="ACHKG5_001714"
     CDS             complement(29604..30440)
                     /gene="rhaS"
                     /locus_tag="ACHKG5_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0867835.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30728..32197
                     /gene="rhaB"
                     /locus_tag="ACHKG5_001715"
     CDS             30728..32197
                     /gene="rhaB"
                     /locus_tag="ACHKG5_001715"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0867836.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            32194..33453
                     /gene="rhaA"
                     /locus_tag="ACHKG5_001716"
     CDS             32194..33453
                     /gene="rhaA"
                     /locus_tag="ACHKG5_001716"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0867837.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            33628..34452
                     /gene="rhaD"
                     /locus_tag="ACHKG5_001717"
     CDS             33628..34452
                     /gene="rhaD"
                     /locus_tag="ACHKG5_001717"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0867838.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            34462..34776
                     /gene="rhaM"
                     /locus_tag="ACHKG5_001718"
     CDS             34462..34776
                     /gene="rhaM"
                     /locus_tag="ACHKG5_001718"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0867839.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(34817..36211)
                     /locus_tag="ACHKG5_001719"
     CDS             complement(34817..36211)
                     /locus_tag="ACHKG5_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0867840.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         8705 a         9701 c         9190 g         8795 t
ORIGIN      
        1 cgagaagacc agccacatcc tttccactca aattagtaag cgtattgtcc agcggttgtt
       61 tgcctgccag tgcattaagc actgttgttg aaaagttagg gtcattcccc agcgccgccg
      121 ccagttcatt cagtgtatcc agtgccgcag gggcagaacc caccattgcc gcaattgctg
      181 atttcacaaa agccgtggtg gcaatctgtg tattgttgac cgactgtgcc gcagtaggtg
      241 ctgttggcgt tccggtaagt gcgggactcg acagcggcgc tttcagtgcc agcgcattgt
      301 taatggtggt gctgaatttc gggtcattgt taatggctgc ggcaatttct ttcagtgtgt
      361 ccagcgtggc tggcgcgccg ttaatcagag cggtaatagc agcctgtaca aacgcagtgg
      421 tcgcaatccg cgtggtgtta tttcctgcat caggcgtcgg cgcttttggt tctccggtaa
      481 atgtcggatt atgtttctgt gcatactggg tatgaggatc ctgtgcggca atgtggtttc
      541 tcatctggtc atccacatac agccttaatt ccaggacttc atcatccacg tatttacggg
      601 tcgccagtac caccgacggg tcgattttca gcgtgatggc ttcggtattc gtgacaacca
      661 gaatcatgcg gatagtctgg gtacgaccac tgccttcctg taactgcggt ttgtacgttt
      721 ccgggcagtt cgccaccgca atgagtacgc cttcatcatc ataaagccca atctcacgga
      781 tccagaatcc gccctcgttc tcagggatga tttgctccgc aataatctgg ctctggttat
      841 tcgggtcaac actcagaaga ttcagcggcg cgatgcgttt ctggttaatc agttttgtct
      901 gtgccgggtc tggtgtcggc aagacaccat tcgcatcacc aacggccatt tgcgtcagat
      961 tcagcttact gccgagcatc gtcgcgttag ccagccgtgc tgcgccctga ttagtcagaa
     1021 tggcgtagta tttcactgtc atgcgtttac tctcaggtta tcaattaaat gaatggccga
     1081 ggccgggaaa taatcccctc cgacaataat ggcctccggg gtgtagggat aaaccgtcag
     1141 ggcgtcaccg tgatagcatc ccgcaccggc aaaaatgttg ccggttgtac ttaaactgat
     1201 agccagtccc gtcagatggc ggcttgccgg ttttgcatca gcaacgagac gctccagctc
     1261 ctgatacatt tcttcggtaa taccctgctc aagcacgcca acaacgatac ggaacgtccc
     1321 cggctcctcg ttgagctgcc accactccct cacctcaatc agatagccga gcggctccac
     1381 cacacggcga atcgcaccaa tagtgccctt atgacagtgg atgaaatacg catcgcggat
     1441 aacagcgcgt tttgtcgctt ccggccaatt atcatcccag cggtcaaccg aaaatgacca
     1501 cgccagccac ggcagcagat ttgccgggca ggtgtccggg ttccacagct cacgaatact
     1561 gaccggcgtt ttttcaattt ccgcacaggc ttttgcggcg gcaacttcaa acggtgatga
     1621 gccggtcggc agcagtcgcg aatcactcat ccgagcctcc ggtcacaacg ctgtattcgg
     1681 tacagaaaga cgcctgcgta ttgttgagca cgatgtcggc cagtggtgca gccagttcga
     1741 cacgctgcac gccttccaca tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc
     1801 ccagccggtg ctgtgccgtg atgtacgctt ccagtttttt cacggcagca gcgcggatgg
     1861 gttcgctttc gggaccaggg taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg
     1921 cggcagactg cacggtcacg cggtcggcca ccggcctgac gtcctcgcca ttaagggcgt
     1981 tacgcaccgc cgccagcagg tcttcggatg ccacaccgtt attttcgcga gacagcacgg
     2041 agatggtgac gcaggccgga gaagggctgg tgacagagat atctgcgaca cgcccgtcgg
     2101 cactgcgacc atgatactga taggcaccca ccgacccggc gacgcttaaa ccttcaaacg
     2161 cctgctgaat acgcagacga taatcggtgt cagattccat cactgccggt gtcggcggga
     2221 tggtcgaatc atctgccggg gtgatagtca ggcgcgtggt gttgtaattg gcaccaatca
     2281 catcaaggtc attaccggcg gcacaggcca gcatcaccgc ccgtgcggcc tcattcacac
     2341 gctgacgcca gataagctca cgataagcat tttcctccag cagtttgacg agaggctcag
     2401 attccagcgt cagggtacgg gcgaccgcct cctgctggtc ttccgggtaa agggaaatca
     2461 gtgtcgcctt gcgttcggca agaatggttt caaagtccag ctcctcgacc acatccggtg
     2521 cgggtagctg gttcaggtcg ataatcggca tggtttcaac tcacagggat ggttaacgaa
     2581 agtggctggc cggtgtcgtt gtgctggccg gttaatgtaa ctgtcattcg cccgtcaaag
     2641 ctgcgcgccg tggtgacgga tgacagggtg acgcggggtt cccatttcag caccgccatg
     2701 taacaggcga ccttaatctg caactcaagt gccggggtct gcggctggtc aatcattgac
     2761 gccagcaacg agccgtaatc acgacgcatc acccgtgagc cgaccggtgt gcgcaggata
     2821 tcgccgatac tctggctgat atgctcaagg tcagtgacag tctggccatc actgcgattc
     2881 attccgagat aacgcgctgt catagaggac tcccggttgt gccgccgctg tcgccggggt
     2941 gtttatgggt atgcagtacc ttaccgtttg atgagagttc accgccggtg tgttcaatgt
     3001 tgccgcgcat cgtcccgccc ttctgcactt ccagcgtgcc ggtaatcagc ttgtttgtgc
     3061 agaccacctc cggtgtgtcc agggtgacgc gggttgatgc tttcaccatg accaccggca
     3121 ccgtggcagt aacagaatca gaagccgtca cgctggccgt tttaattccg cttgccgtga
     3181 gtgcactggt ttcaggttca tactcaatca ccgccccgtc aggaaaacgg atatgcaggg
     3241 catccgccga cgcagacggc gcggggttat caccggaata aatccccggc agaacgaacg
     3301 ccgtgtcgag ttcaccgccc acggccagaa tcagcacctg ttcccccacg gaaggtgccc
     3361 accatgtgcg cgaacgaccg gcgcgatggg tcagccactg cagccagtcg gtacacatgc
     3421 cgccggtctg cacacggcag cgaccggcgt taaggtcggt ttcgacgata acgccggtgc
     3481 gaatcatgtt gcgcagtgcg cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg
     3541 gaaggatgcc gccgggtcat accggcggca atgtgacgat gaggtgtcgg gaatggcaca
     3601 actaacggtc gaggtgcgcc agaataatct cttcaatcat ctgcacatcc tcaccggtga
     3661 agccgagcag agggcgcgcc ggataatcaa ttttcttacc gtctttccgg ttttcttccg
     3721 acagaccaaa ctgatgcaca ctggcgattt tcggtgactt cccgccgtaa aattccattg
     3781 ctgcctgttc cgggctggcg cggatatgca aaaaacgact ggtgataagt ttcgcaaaca
     3841 tttttcgctt aacgcgaccg gtcttttttc tggcgctctg ctgctggcgt ggcgcgtagg
     3901 gtgtgccgtc cggggctttc tgtgccatca cccgacgctg ctgactctgc cgcagacgtt
     3961 tcgccagttc ggcactcagt cgccgacgcc ctgacggtga cagcgattca atcagtccgg
     4021 tcagccggtc ttcaaaacgc ttaaactcat tcatcccact tgctcaccag ttcgccattg
     4081 atataaagct ccatcgggcg ggtgaccggc tccggcggcg tgggttccgg gatattcttc
     4141 acatgcagcg cgccgtccac ctcactgacc agcgtgcgct cggtcagcat caggctgatg
     4201 ctgatatcaa agctgctgtc attgttgatg tctgcataaa acgtgaaacc ttttttctgg
     4261 ccttcgtcgg tggtcatgat gtcgggctga ttttcccgca gccacgccag caccggcacg
     4321 atgagcaggt caaaatcacc ggtaaagtcg gtcacaatga cattgagcgt gtaacgtttt
     4381 tcgaatgaca acgacgccgc cagcgtggag gcaatactcc cgttatccac gaatatccga
     4441 agcatctctg gactggtttt cagcaccgtg acggcatcag tcagcgccct gcgcaggctg
     4501 tcgggtttga gcatcgtttt cgtcctgaca gtgtttaatc atttttacct ggctggcaca
     4561 gcgtgccagc gcgttctcaa gctgccggat atcggcactt aaatcgccgt tcgtctgcgg
     4621 gtcactgccc ggcatcgggc aaaggctcac tttcgggcag gcgttggcga caatcactgg
     4681 cgtctgcgca ggcggggcgc tggtgcaacc ggcgcacagc atcaggcagg tcagcgccgt
     4741 accagcggcg aaaatcttcg ttttcattaa gtaacctcgt gatggttttc tcgcgctgtg
     4801 cttcacgctt cgcggcgttc tccagttcct gacgcagtgc cacctgcgcc agctcgtttt
     4861 tgtctgccct ggtgagggca acatgaagct gatttttcag catggtgatg gtcgcctgct
     4921 gttcactggc gacgttgttc gccctgtcca gcgaggcgcg caggctggca tttttgtgtt
     4981 tcaccagaaa cagaccggcc accgccagcg ataacaacac gaccagcaca atcatcagct
     5041 ttgacatggt tcccgcccct caaaacgctg acggcaggcc gtacgtatca gccggaagaa
     5101 caccgatgcc acgagataaa tcagcgcggt aaaaatccac ccggcagcaa ccagcgagat
     5161 aaacgtcgcc accatcacta ccagagccac tgaccgcctg cgccacggca ccggctgcaa
     5221 aaacagcgcc gtgacaattt tcacggccag cgattccggc ggcagctccc gcccgtagcg
     5281 ttccagcaca tactcagtgg catacacccc gacaccaccg gcaaccacac agataaccgt
     5341 cgccagaatc gcccaggcag cgacaaaact gacggccacg ctctgcggat aaatcaggga
     5401 cagtgccagc atcagcgcca gcgacacgtt cagcatcagt gaaagggata atttcttcat
     5461 ggtgtttact ccgtttaagc cggtacgccg ccagcggtac gccagacggt gaccagtttt
     5521 tccagtgaat gctcacgctg accgtaaccg gcacccggca gggacgccca gatattgcga
     5581 cagcgtgaaa tggcgcgctc aatgcgtccc gcccggatgt catccagtgc accgcgttcg
     5641 cggatcaact gaatggcgag tctgtcctgt gacaacggac tgaaatccgg cagggcaagc
     5701 tgtttgcggt aatgcggcca gaacaggtaa agctgctgat agcgaccgga ggccgtggat
     5761 ttttcaccgc gacggttaaa caccttcgcc ggtcgaccat gtgcgaacgg gtggtcactg
     5821 tagtcggtga aaatttcctg ctttccgtcc agtccggtga ctatcacgtc atagccccgg
     5881 tttttcgtca gcggatggtt cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg
     5941 gcgatattct gatgcgtgtt aattaccggc attacggttt ccccctgctc ttaaagcggc
     6001 gctgaatggc aatctcaatc acctgataac cggcgatacc cagcatggag ccgatgccgc
     6061 acaccgcagg cagtgacagg tcaggaaact gcaccagaac aacaccggca accatcgaga
     6121 caaaaccacc gagcaacatg cgcccgataa acagacgcgg ggtgatgggt tcaccaccgg
     6181 caagcacctt gccgacaaca atcagcaccc caatcatgaa aagcgacagg acgctttttt
     6241 cttctgctgt catgcgttac tcccacagat tgacagtttc agccacgggc gcggtctgaa
     6301 cgtcgggcag ttcgacggcg gtgccatgtg gcagcaccgc ccccagttca gccagtcccg
     6361 gatttgcggc gagcacggct tcgaccacgc cctcagtgcg cccgtaatac cggacacaaa
     6421 tggcgtcgag cgtgtcgccc tgtagcgcaa aggtcttcat cagatttgac tcacgatgca
     6481 gcgcggcttg tcctggatac gcgccaccgc ccagcgcata tcccgccaca gctcatcaat
     6541 ggtgctgtca atgctgtcgg ccttcttgtc gcctttcgca ctggcatcca cgccgcgata
     6601 acgctcataa agcgatgcgg tcgccatcgc acacacggcg cgctcgtagt aaaaaacttt
     6661 gatgctttca ccgtcgatgt cgtccgccgg gacgtccgcc agacgcgtaa aaccggcggc
     6721 aattttctgt tcgcggtact cgtacagctc cgcattcgtt tcagccatgc ctgacttgat
     6781 ggcctcacgc agacgggcgg gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg
     6841 cttcgggtcg atatcgggaa aaaagaacgt gtttttaatc accggctcgt cgcctgccgg
     6901 ttgcgggatg accaccgtac cctcaccgga cacgggagcc tcctttcgcg gaataatcag
     6961 cgtcatcatg actacctctg aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt
     7021 caccctcact gaccggcgtg ccgccctggc gcggggcgca ttcggttgtt aactggcttt
     7081 ctttttcggg cgtccacgtt ttgccggtgt cacgctccgg gtcttacgcg gggcacgggt
     7141 ggccgctttt ggctgcggct ccggcttcgg tttcagctcc cgctccagtc gttcaatctc
     7201 ttttttgacg cctgcctgac agtcgagctg tgtcgcacgt tgcaggtgag ccagcgcacc
     7261 ggcggcatca ccagcgtcac gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac
     7321 ttcatcaggc atgtcagccg tggcggtcag ttcaagggtg tccgtcagca ggcgggtatc
     7381 cacagactca ccggcagcgt gggcacgcat ggccgcaagc gcgacctcct cggtgaacat
     7441 gtacggcggg gtgcggcggt gttttcccgg catggtcaga ccgtacttca gggcataacg
     7501 ggcaatctcc agcgcaccgg caatatcgcc ggtatccaga cgccacagca tgaccgtcat
     7561 cagaatgtca tcctgtgcac ctttgccctg ctccagaacg ccgttcaccc acggcaacca
     7621 gaacggcagc agttcgcgtt ttttcgcggc cttcagctct tttgaataaa tcgctttcag
     7681 tgtgcgctgg tctgcggcga gcttaaccag catctgctca tagacagttg catgtcgcag
     7741 cggggcggct tcccgctgcg cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc
     7801 gggactcgtc atcggttacg ctcccggctc tgcggtcgcc ttagccggtg tggagaaatc
     7861 accgacctta attttttcca ccagacaacc ggcggcgtag tcttccacca cgtaatcaat
     7921 gttcattgac tcgtagttct ccacgcggtc gagtttcggg ttttcctcaa tcacgcggcg
     7981 atggctgtca tccatgtagt agatggacag gttttccagc ttcgtgatga gcatcgcatc
     8041 cgccgggaag tacgggacgc gtaccgctgg caggttaccg atgcgtttct ggctgatgat
     8101 gacgtcagcg gccagcattt cgctgttgtc ctgctccttg ttaacgatgg ggaaatactt
     8161 gtccgccagt agctgacgcc ccacaatcac cacaaggtca gggtcttcct gataccacgg
     8221 ctcaatcagg ttgttggtcg catccatcac cagtgcgtcg aggctggcat aatcaccgcc
     8281 cttacccacg cggatgacct cagaggtggt gcgaccttcc tcgtcagtga ccttgctcat
     8341 cacgcgcgcc ggggcttcat tgcggtattt ctgcagccag ccgaccgcca catcctgcaa
     8401 catcggattg ctgctgcggt cagaggtttc ggcacgcttc acgccgttaa aaccggccat
     8461 gatgaaatca agggactggc gtttgataat ggcgttacgg acacggagct ggaaatcctg
     8521 ataacgcgcc cacaggtcaa gcgttttgta gcggatataa aaatcgaagt taatctggtc
     8581 gcattcgtac ttgtttgacg ccagcttcga gaagtccttc ggctgacgct cggtgccacc
     8641 ggcggtgtcg gtggtgctgg cgatggagcc ggtgacacca ataccaattt tttccccttt
     8701 catttcgctg accggcacaa tgttgatgcg ggtcagaaag tcagaggact cctgcatggt
     8761 gttcatcagg gtctgggtga ccgaaggttc aacggtgaat tttttcgaca catcaccggc
     8821 gtcgatgccg ttcagttcgg caacacggga caggtaggca ttaaatttaa agcgggtttc
     8881 ctggcgcata gttattcctg aaattaaggg ttaatcgtga aggttttccc ggactgacgc
     8941 cggtcagcag ttcgtcatca gggcgtcacc gccaccgccg gtggccttgc tgcggcgctg
     9001 ctgggtcaga ctttcggtgt ggtcgagact gtttttcagg cgggtgaatg cctggctggt
     9061 ttcatccgcc ctgtcagtca catcctgctt aagtgcggaa aaggcgtttt ccatctcagc
     9121 gaggcgctgc tcagtggcgc tcagtttttc ctgcacatgt tcagcaacag cggtcaccgc
     9181 ttcatgcacg tcattcagac gggcgtcatc gctggcctgt ttgcggccaa aaatggactt
     9241 caccttttcg gtcagggcgg tgaacacggt ttcaggcagg tcttcaaatt ccagctcaac
     9301 aggcgttgcc actgaaatca ggttttcagg gcttaatttg aagcggttca gggggttgtg
     9361 ttttgccgtg cggcagaatt ccaggtattc cgtgccgagg cttgccgggt catcggtgac
     9421 ggccagaccc accagataac atttgccggt attggcaaag ttcggctgaa tttccattga
     9481 ggtatagacc ttctgcgcgg ccttgttcat cgcgataagg tcatcggtcg gggtgatttt
     9541 cgcaaacagc gcccatttgc ctttcagcgc cgaatcatcg tcaatctttt cggccttcag
     9601 ttcgaccaca tcgccataac gcttaaaaat accgtcaggc aggatgccgc gcagatgttc
     9661 caggttaatg cggcaaccat agactcgcgg gtcaaaggtt tcggccattt cctgaatatc
     9721 ctgcgcactg atgacacgcc cgtcacaggt gtcaccctca acgccgatac gaaagaattt
     9781 tgagactttt tttgccattg tcaggagtcc tgaatagtga ttagaggagt cacatgtcgg
     9841 catcagtttc ccgacgatgc gcatcctccg ccatcagtcc cggatggctt atcactgaca
     9901 caacagcacc ttagcgaatc gcggggcgcg actcagtagc cttgccgtgt attcatcacg
     9961 gcgaggtatt catgaccatc accacagaca ccactctttt acacgacccg cgtcgtcagg
    10021 cggcgctgct gtactggcag gggttttccg tgccgcagat tgccgccatg ttgcagatga
    10081 aacgcccgac agtgcagagc tggaaacagc gcgacggctg ggacagcgtt gcccccatca
    10141 gccgtgtcga aatgagtctg gaagcgcggc tgacccagct catcatcaaa ccgcagaaaa
    10201 ccggcggtga cttcaaggaa attgacctgc tgggacgcca gattgaacga ctggcacggg
    10261 taaaccgtta cagccagacc ggcaacgagg cagaccttaa tccgaacgtc gctaaccgca
    10321 acaaaggcgg gcgtcgcaaa ccgaaaaaga attttttcag tgacgaggcc atcgaaaagc
    10381 tggagcagat tttctttgag cagtctttcg actatcagtt gcactggtat cgcgccgggc
    10441 ttgagcaccg catccgcgat atcctgaaat cccgccagat tggcgcgacg ttttattttt
    10501 cccgcgaggc gctgctgcgt gccctgaaaa ccggtcataa ccagattttt ctgtcggcca
    10561 gtaaaacgca ggcgtatgtg ttccgcgaat acatcatcgc ctttgcccgg ctggttgacg
    10621 ttgacctgac cggtgacccg attgtcctgg gcaataacgg cgcaaaactg atttttctcg
    10681 gcaccaactc caacaccgca caaagccata acggcgacct gtacgtcgac gagattttct
    10741 ggataccgaa ttttcaggta ctgcgtaagg tggcatcagg tatggcctca cagagtcacc
    10801 tgcgctcgac ctatttctcc accccgtcca cgctggcgca cgacgcctac ccgttctggt
    10861 ccggtgaact gtttaaccgg ggacgcgcca gcgccgctga acgcgtggaa atcgacgtca
    10921 gtcataacgc ccttgccggt gggcttctct gtgcggacgg ccagtggcgg cagattgtca
    10981 ccattgagga cgcgctgaaa ggcggctgca cgctgttcga cattgagcag ctcaaacgcg
    11041 aaaacagcgc cgacgatttt aaaaacctgt tcatgtgtga atttgttgac gacaaggcgt
    11101 cggtgttccc gttcgaggag ctgcaacgct gcatggtcga cacactggaa gaatgggaag
    11161 actatgcacc ctttgccgca aatccgttcg gctcccgccc ggtatggatt ggttacgacc
    11221 cgtcacaccg tggcgacagc gccggatgcg tggtactggc accgccggtg gtggccggtg
    11281 gcaaattcag aatacttgag cgtcaccagt ggaaaggcat ggactttgcc actcaggcgg
    11341 aatccatccg caaactcacc gaaaaataca acgtcgaata catcggaatt gatgccaccg
    11401 gcctcggtgt cggcgtgttc cagctcgtgc gctcgttcta tcccgccgcg cgcgatatcc
    11461 gctacacgcc ggaaatgaaa accgcaatgg tgctcaaggc caaagacgtc atccgccgtg
    11521 gctgtctgga atatgacgtc agcgccaccg acatcaccag ctcgttcatg gctatccgca
    11581 agaccatgac cagcagcgga cgcagcgcca cctatgaggc cagccgcagc gaggaagcca
    11641 gccacgccga cctcgcctgg gcaaccatgc acgccctgtt aaatgagcca ctcaccgccg
    11701 gtatcagcac tccgctgaca tccaccattc tggagtttta ctgatgagca agaaaaaagg
    11761 gaaaacaccg cgacctgcgg caaaaacaat caccgccagc gccccgaaaa tggaagcatt
    11821 cacctttggt gagccggtgc cggtactcga ccgccgtgac attctggatt acgtcgagtg
    11881 catcagtaac ggcagatggt atgagccacc ggtcagcttt accggtctgg caaaaagcct
    11941 gcgtgctgcc gtgcatcaca gctcaccgat ttacgtcaaa cgtaatattc tggcctcgac
    12001 atttatcccg catccgtggc tttcccagca ggatttcagc cgctttgtgc tggattttct
    12061 ggtcttcggt aatgcgtttc tggaaaagcg atacagcacc accggtaagg tcatcagact
    12121 ggaaacctca ccggcaaaat atacccgccg tggtgtggag gaggatgttt actggtgggt
    12181 gccgtccttc aacgagccga cagccttcgc gaccggctcc gtgtttcacc tgctggagcc
    12241 ggatattaat caggagctgt acggcctgcc ggaatatctc agcgccctta actctgcctg
    12301 gctgaatgag tcggccacgc tgttccgccg caaatattac gaaaacggcg cacatgccgg
    12361 atacatcatg tacgtcaccg acgcattgca ggatcgcgat tacattgact caatccgcga
    12421 aaacatggct aattcgaaag gccgcaataa ctttaaaaat ctgtttctct atgcaccgca
    12481 gggaaagcca gacggcatca aaattatccc gctcagtgaa gtggcaacga aggacgattt
    12541 ttttaatatc aaaaaagcca gcgccgctga cctgctggac gcgcaccgca tcccctttca
    12601 gttgatgggc ggcaagccgg agaacgtcgg gtcgctgggt gatattgaga aagtggcaaa
    12661 ggtctttgtc cgcaatgagc ttatcccgtt acaggacagg attcgggaaa taaacggctg
    12721 gctcggccag gaggtcatcc gctttaaaaa ctactcactg gacactgaca acggctgaac
    12781 atcgccgcct gcgggcggct tttttacacc ccgtcatcac gccctcacac gctcaccacc
    12841 gcacaaaaca ccccgcagac acaccaacgc cccggcgcac actctaaacg ccatcacgac
    12901 gcgctcagac gctgaaaaaa taaaatcagc accaccgcca gcgcgcagtg ctttccccgc
    12961 ctcgcccgcc cgcttcatgg ggcggtttta atgcagatgc acgaacacgc cgaaggcgcg
    13021 ccagtaccgg cggcgctcac acgtgactgg gaaggaaaac gcatgcgatt cgatgcagta
    13081 atgcatgcag agctaaaaat ggcagaacat actgaaaaaa cgcataaaaa aaccggcatt
    13141 caggttgccg gtttgagtcg acttcattgt ggttactggc cgcgcaatgc gccaataata
    13201 ctggtaaggc aacaagtggc aacaatcagc aaaaaaaccg tcgtccacgg attttcataa
    13261 atgagagata acatattgat aaagatcctt ttatttgttc attctgatgt ggtttaaaag
    13321 gtgtttgata acgaccgtaa gctcatcttt actggcttgc tcgaccattt tttcggtgta
    13381 gctttccact tcgcgggaac tcaggtcgtt atttgaggcc attacagtga gtctctttgc
    13441 ccagtcggca taagggtctt taattgatga aagggaatta tgcatgtcca aaaacctcga
    13501 attatttaac cagcagacgg ctgaaatctt tgcggtgcta tgggataact ttcctgtacc
    13561 acaagtcatc acctacaaaa aatttaacgc cgcgttacct gatgactact ttgaccaact
    13621 taactcacca gaaatgaaag cactgaatca gttgcgtagt gtggttgatg gcacattcac
    13681 tttcctaagc gaaaacggct atattctgta cggaacagac catcagaccg gttttcatga
    13741 tgtgcgcctg accgagaaag cactcgcggt gctcaacaaa aaacccgaag cacttggcgg
    13801 taatgaaacg atgggagata aaattatcag tgcagtaaaa gacgggactc caggtgtcat
    13861 tgctggagca gtgacaaacc tgttgactct tggtgttaat tttgtgacgt cagcttcata
    13921 gctaactact ttctgaggtt tctaatcaac gccgcctgca ccgaaagtaa attcctgcac
    13981 tggcggcgtt actaacgcca aagttatttt aattattgat gctattatgc aatacattca
    14041 atatgttaat ttgcagtatt agcctttaaa gtaagcacct tagatttgta acgttgctca
    14101 atcactccca ttgaatgcaa acgaccaaag agctttttgg caatgattgt ttctctcgca
    14161 ttttcggcgt ctttaattat tttccaataa tctatttccc tagccatcaa aaaatgctgc
    14221 tcattcctaa cttttaatgc attgcctaat acaataactc cagttaccaa cttaggctta
    14281 tctctggcat atagttttgt tttcgttggg tgtataacat gcccatgctt attaacgatc
    14341 ttacgaatta cagcgcagaa caatcgattt acgttactac ctgaaaaagt gagatcatca
    14401 acataaacag tcatgttaac tcgtaacttt tggcaaagtt gatatatttc accaaacatt
    14461 cttgaatttg caaaatatgc caacggcata ctgatgcgac tacctgttgg caatcggtca
    14521 tgacaagtac atatgtgaga caaaacatca gcaacatcag atgacatttt catcaccgaa
    14581 aagaaaaaag aaaatatcat ccctcttgta gttgatggaa aaaaagcttt aatatctgtt
    14641 gtcatcatct tttcattatt aagatgagct ttagcattcg tcacattaga gcacttcttc
    14701 ttccctgagt gcagatattc tggcaaagca atccgcgata acaaacttgc aattcgtgta
    14761 tgtacaagtt ctagtttttc taaaggcttt tgtatttttc gtgctttccc ttttttagaa
    14821 agctgctcaa aaacggaata gtttccttca tcttttctca aagagtataa atcatcaaga
    14881 ctaacacaaa gcaaattcgc cagttttctt ttacttttga gtttataaaa tggcgaatcc
    14941 attatgtcat aagatttatt tttagtatta actttaagct ttttccgttt tttcattttt
    15001 attacttacc cactccagaa catccagaac tttaccggct aaatttaaac gcaatcgctt
    15061 agacaaacgg ccttgcgtgc ctaatgattc cgaaaagaat actaaagagg atactggtat
    15121 gtcgaaatgg gaggcgtaac gctctaagac ttcaatcgac ggtgaccaaa ctccgctttc
    15181 aagcgatact attttgtctt ttggaatacc taaagatgaa gataactcaa ctattgattc
    15241 tttatggtat tgccttatta aacgaagggc tttatgtaac ataggcaact ccatgctatt
    15301 gaaactaggg aggacgcatt actcatcacc cccttcgaag tagtccagtg ctcgagtaac
    15361 taaatcaaac agtctaatac cccatttgat taatttccaa gcgtaaccag cccacttttt
    15421 caggtttgac ttgttctgcg cctgctgttc gttggttaat ttatcattct tcatgatttg
    15481 ctccttatgc ccatagtgtg caaacggtat gtctgctcca acactatggt tcgccctcat
    15541 aaggatgaga ttgaacagca ctagccccct gctccacgaa cccttggtcg cccaaagggt
    15601 tcgaataccc atacgccata cctcctgatc ctgtgactcc tgtcgctggc aggggcgttg
    15661 cgcggggtaa gtgttagaaa gcgttaactt tccacgggta aaaataccca attgatggcg
    15721 atatatctca ctaactaacc aatgtttgca tcataacaag taaaccgttg tatttgctcc
    15781 ccctgtatat gatatttttt catataaaac aaatatataa taagttaaag gcatatcaat
    15841 gcaacatgga tatgctttca ttgcattgaa ttttaaagtg ttttacatga aaaatgactc
    15901 ttcatctcga aatccgaacc actcattaat aaccatatac gtgaattttt tcccgtcata
    15961 atttacggtt gccccacgcg ccagcgcctc aatctcccat cgctgaggcc tgataccgtt
    16021 ctgagcaagg tcaacgcgga tacgggtaat ttgcattcgt tccgaccggg tcagtctggc
    16081 cgatggcgct atttcatgcg gttttaacgg gcttccgctt ctttgctgac ggtttggtgt
    16141 tctcaggtcg tgttttaatg cgcccctgag cgccctcacg acctccgggt cattccattc
    16201 gataacaccg tcatcaacca gattaagcac tgcggcggcg tgctcagaag gtgtgggagc
    16261 cggtaacgaa gtatcaccac cggtgagctt tccacagtta ttgacaggac tccgaggcgc
    16321 ggcgatgccg ctttttaaag tcaaaggctc cacgaccgga actttcggca caatgcgcca
    16381 gtccgtcgtt ctggtgatat gaatatgacg cgcgccgaga tgcggcgcgt aaatgccgac
    16441 cactctctcg acttcttcct cgtactcgtt aacgtcatcc gacgggctac gggcgactct
    16501 gacagtctgg caatcgcgcg gaacatttgc cccaccctgc gcgctgatat acaacgcaaa
    16561 atcgccactg tctgcggcag cacgtgcagc ctcgacgcgt tcgtcaaact catcagcaat
    16621 gctgacgccg cgaggcaatt tgcgtagttc acggtaagcc cccattgtcg gcaggccaac
    16681 cgttttaaat tgcgggatac gccacgttga cgcccatgcg gtaacagccg ctgcagtgtc
    16741 tttcagcggc ctgccggtat cgttatcgag ctgaccatcc agtgcatagc cgtcgatgtt
    16801 ttttgaaatg tatttcgcga tatatcccgc tgcaccgccc cggttaaggt gttttgcctg
    16861 aaaacggttt cgcgcagctc ctctttcatc gccatcctct ttgagcgcat agcgacgcat
    16921 gatttcgata atctggttac gctggcgtgg attacaaaaa agcatcatat gccagtgcgg
    16981 tgttccatcg tggtgtggct cgacgactcg caaaccgtag acctgtaaat cattatcctt
    17041 gaatgccgtg cgcatcaggc tccagatacg gcagagataa cgctgcgcat cctttggatt
    17101 aaatgcctca tcgttccagc cgtgatttag ctggacggtt ttactttcgc cttttccgac
    17161 ctgacgtgtc gggtgatact ttgacggcgt ggtcagcgtg ataaacatcc ccacatcacc
    17221 ctctgcggcg gcgtaacgct caataccggc aatggtgttc atcagctcca tccggcgaat
    17281 ttcaggatta gaaatactgc ccatcacctt actgataagg tcgatgcgtt cgccggtttc
    17341 cctgttttcg aggtcacacg atttaagaaa ttccagattt gcctggcggc gcgcacgcac
    17401 atcacgaatg gcgtgtttac tggcataagg agaacggtct ttattgacct ccccgacagc
    17461 aatcagtaac gcctcatgcc agcgcatacg ctggccttta agctgatgag tccaccactc
    17521 atcgttaaac aggcgggcaa tggcagaata tgcctgcctc gtggtcatct gccctttacg
    17581 gtattttttc cagtaaagcg gggaaatatt gaaagcacgt gcagcgccag caacatgacc
    17641 atacaggtga gcctgcgcct catccgtaaa cagcgattct ttttcgccat gcgcatccac
    17701 ccaggcatcg cagatttcct catacatcat gaaaagctgc gatgagatac gggcggcaaa
    17761 ctttttcagc tccttgtcat tcattccagg caggcgcgca tagtggtcac gctctgccag
    17821 aaacagcaac gacgcgtcgg tgttcatttc atggcgctga ttcacgcgct caatgcgcgg
    17881 ccataaacga cgctgaaaag tggatgtgag gaaataaaac ccgtgcaccg ggcttttatt
    17941 gcgccggata tagtcatagc gtgaagtaaa cagcgagcgc aaaaagtaag gcaggcggtt
    18001 aatcgtggat aaaacacctt gcacctgacg catctcgtca cgtgtaaggg gtctttcgcg
    18061 cccgacagcc tcacgtggcg cgttccatgc ataagcaccg gtaaacgtct taccggtgcc
    18121 tgcggcaaat gctgacgaag ggacaaaacg cccggaggct ttaacggcca tgtgtgccaa
    18181 aagcctctga acaacgcttg ctgagttgct caacctgcgc gtttaaatcg gcaaaagatt
    18241 ttgcgcttcc ggtcagaata tcgtgatgca tcaggccgga aacgagctgg cttaatttcg
    18301 ggtaataacc aaccaccgcc agccattcct gaccggcgtt tttaccgctt tccgctctct
    18361 ttttctcgtg gagaataaac tgaaagctgt cactggtaac gacataacgt tcgccaattt
    18421 caatacgaat actcatgccg ttctccggta atgtttgttt tttgcttcaa agactgactg
    18481 gcaggaaaca caacgcgtgg ctgacggata agccgcacga cgggcagcag gtattggcgc
    18541 atcacactct tcgcaaacca gcgcagaagc acagcaatgt tttacccttg ccgcgttaat
    18601 ctggcgctcc agtaattcag cctgttgttc ctgaatgaaa tctacgttgt ccggcattac
    18661 cagttccttt tgtcgttcag tttcttaaat tcatcagcgc aatagctggc gagttctgtc
    18721 gttaatttag tcagttcatc cacggaggag atttgcttgt ggaatacagc gcgtttaaca
    18781 agtaaattga ccacatcaga caggaggttt aattcattct gataaatcgc gataacagat
    18841 tcagttatgt cgcgcttttc tttatcaaga ccaagttgaa taagagacaa atcgccattt
    18901 tccataacgg cgatttttaa ggcgttattc agtaatacaa ctgaatgaga acaggacatc
    18961 aaagcacctc cccgcgagac aatccgatat tgtgaaattt ttccgactcc tgactgagca
    19021 gctcgactat ctccacgcgg gataactccg cctttgtgat atggcgaatc atggcgtcaa
    19081 gatgagaaga aaagcgtgtc gctgcgtcgg cctgtgcttc ggctctggcc tgttgcagca
    19141 gtaatgcgta tttaccgcac ttgttttcag aaactgtatg catgactttc tccaggcaaa
    19201 aagaagcccc gcacgattaa gtgcgttaaa aactctggtt aattatttaa tgcagatatt
    19261 gctctggttt taccgacgtc agaattgtcg gtgcatactc aaacaggctg aataattcac
    19321 gtaatgcacg gaataaagca tcacgccagt aacatgattc ttcattaatt cgccagtatg
    19381 gctggttgaa ttctttttca gtcaatccgg catgcataaa taaagtacga cgctgactga
    19441 ctgttaaaaa actaatatat gcatactcac ttgcgccaac ctgacggcgt tttgagaatg
    19501 ccccacgcaa ttcatcaatt gcacaaacca gccgttcacg ttcgacgtcg ttcatttctt
    19561 caaaacgcat cgttgcgtga cgctgtttta actgcgcatg gaagcaaacc gttagccgtt
    19621 cgcgttccat catctgatta taataatcac atgtatcctg ccagcgaggg acagcaagat
    19681 gcttgccaat tatccggcgc atagctgctg gctgtttttc aacgagattg agcgtcatca
    19741 ctgtcatttc cagaccctcc ggcttttcag aaaggtcaga gcctttttta acggactctg
    19801 ttttttggtg cggataatga ttcccttgcg tcccttcccg tgggtgattg tgaagtcaat
    19861 cgccctgggg ctttcgttac gcaataactg agcaatacaa cgcggctcat tcataatcac
    19921 aaccccatcc acaaaagcca tgcatcacgc tgttcaaccg gtcggttata aaacgcctca
    19981 cgtacagcgc gattaaactc aggaatgaaa acccattttt caccggcacg agccttcggt
    20041 ttgcaaggat cacgcaattc aataattggt aatttatttg ccttcaccat ttcactgaca
    20101 gctgtctttg gcttccctaa taaatcagca aatttatcca catgaaccgc atcaagcgga
    20161 tactgaatca cataattttc agcgtccata tatggtaccc tcataggatc cagccctttc
    20221 taaaccactc aaaaccgttt agacgctggt ttattctcaa atcaatggaa cctatatagg
    20281 ttccagtttt gagggaattt agtccctata taggcaccat gtcaaatgaa attaagcgaa
    20341 aagattaagg ccttgcgtga ggctgaaggg ctaagccaat caaaattctg tgaaatcata
    20401 gagttaccgc taagcacact taaaaaatat gaaggaggaa actttgaacc cggtggcaca
    20461 gctttgctaa aaatcactat gcatcccaca ttccaaaaat atgctctatg gcttatgaca
    20521 gataaaaccg cgccggacgc aggacaaatc gcaccggctc tcgcgcacat tgggccagag
    20581 tcaacagagt ccaaccactc cgcgaaaagg attggctaac tctatataaa gattacattt
    20641 tcaccatttg ctaccaagat ggtgaataca gcgccggagg gctttcttat ggcaattaag
    20701 aagctcgatg atggtcgcta tgaagtggac attagacctc gcggtcgcga cggaaaacgc
    20761 atccgcagga aattcgaaag aaaagctgaa gcactagcat ttgagcgata cacaatcgcc
    20821 aatgccagtc agaaagaatg gggaggccag cgagcagacc gccggacttt gagtgagttg
    20881 ctggacatct ggtggaaata tcacgggcaa aaccacgagc atggaacaaa agagtttaat
    20941 catctactca aaaccatcag cggcataggt gatataccag tgagcaggat gagcaaaagg
    21001 gctttgatgg attatcgttc catgcgacta cgtgatggta tcagtgccgc aacgataaac
    21061 cgtgacatgt accgattatc cggcatgttc acaaaattaa ttcaattgga tgaattttcc
    21121 gggcaacacc caattcacgg actgccgcca ctggcggagg ccaaccctga aatgacgttc
    21181 ctggaaaaag cagaaatcga aaaactgtta aatgttttgg ctggtgatga cttacttgtc
    21241 gcgcttttat gtctgagcac tggaggaaga tggacggaag ttgccacgct aaaaccagca
    21301 cagattacaa attgcagggt taccttcctg aaaaccaaaa acggtaaaaa gcgaaccgtg
    21361 ccgatttctg aggaactgga gaaaaaagtt aaagaggagg ccagcgccaa attattcaaa
    21421 gttgattatg agaaattttg cgggatttta cgcagagtga aacctgatat accacccaat
    21481 caggcaaccc acatcctgcg gcatacattc gcaagccatt tcatgatgaa tgggggcaat
    21541 ataatcgcac tgcaacagat tctgggacat gcgagcattc agcagacgat ggcctatgcg
    21601 caccttgcgc ctgactatct gcaaaatgcc gtcgctctga atccactaaa aggcggagtg
    21661 acgttataaa tttccccctc tgagtgtcca catagtgtcc acactcaaga aattttgaaa
    21721 ccgctccaaa cccttacagc tttcgataag tacctgtttt tacttttgat tgcatgtaag
    21781 taattgataa aaaaaacccc cacatcatgt gggggaagac agggatggtg tctatggcaa
    21841 ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct tcaacgatga
    21901 acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt
    21961 taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga cttttgccat
    22021 ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc gcacagcgtt
    22081 ttcatcaaaa ttttctgcgg tgacaagacg atgcattgtc tccagttcgc taacattaac
    22141 aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac gctgatgttc
    22201 ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa gttcttcacc
    22261 cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac tgactgccag
    22321 cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa atctttctgt
    22381 cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa
    22441 acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc ggaggtattt
    22501 aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc ctattaaagg
    22561 agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa caggcgcttg
    22621 atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg aggaaaaatg
    22681 gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt atgttgacgg
    22741 cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat gactatctcc
    22801 cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc
    22861 actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa gttgatgcct
    22921 tagtactgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg gagttaaccg
    22981 gtactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg gtttcccgtg
    23041 aacatttaag ccaggaagtg ctgggcaaac gcctgacgcc tttcgaccgc gctatcgata
    23101 tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac ccgtggttta
    23161 aaaccttgcg tggtcgcggc tacctgatgg tttctgcttc atgataggca gcttaaccgc
    23221 gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt
    23281 acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc gtcagggtct
    23341 gatgattgag cagcatgttg aagcggagct ggcgaacgat ccgcccaacg atttaatgtg
    23401 gtggcggcgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc gtttgttatt
    23461 ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa
    23521 ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg gccgcgtgga
    23581 actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt atctgattcg
    23641 tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc cgctattact
    23701 gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct ggagtctggc
    23761 aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa acttacgcca
    23821 gcacccggaa ctggaagcgg ggccacagga attccttgcc gcaggtgcca gttttaacca
    23881 gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt ctgatatctc
    23941 tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg
    24001 tagtggtgaa agcaaggaac tggagcgtat tgaaaccgaa gcgcaacgtc tggacagcat
    24061 gattaacgac ctgttggtga tgtcacgtaa tcagcaaaaa aacgcgctgg ttagcgagac
    24121 catcaaagcc aatcagttgt ggagtgaagt gctggataac gcggcgttcg aagccgagca
    24181 aatgggcaag tcgttgacag ttaacttccc gcctgggccg tggccgctgt acggcaaccc
    24241 gaacgccctg gagagtgcgc tggaaaacat tgttcgtaat gccctgcgtt attcccatac
    24301 gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg tggacgacga
    24361 tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct atcggaccga
    24421 tgaagcgcgc gatcgtgaat ctggcggtac aggtttggga ctggcgattg ttgaaaccgc
    24481 cattcagcag catcgtggct gggtgaaagc agaagacagc ccgctgggcg gtttacggct
    24541 ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg caggataagg
    24601 cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat
    24661 caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc
    24721 cgctcagtcg gcgcttttgc atcgttcgcg tccagctttt cgataacccg gcagcggaga
    24781 gtaaacggtg atactgatcg tcatcaaacg gcatatgcca tgcgatggca gcagcctcct
    24841 gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca gacactttcc
    24901 ctggcgctat cacgctatac aaccatccca ctttacccgt gttttgcatc aactgcgcta
    24961 tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct
    25021 gaattaatgc ctctccccag cggaaaatat cgccaatata aacattactt tccgtgagac
    25081 cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa
    25141 attcccgcgc ccagtagaga taatgctcac gaggataatg acacagcgct ctgtctggtc
    25201 cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc gttagcatca
    25261 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
    25321 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
    25381 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag
    25441 gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag
    25501 gtttatttca ccgtacctaa aatttcgatt ttcgccgctc tggctttgac tttacgtgac
    25561 cagatagagg taagaattgg caccagaatc gaagtcacaa tgactgccgt cgctaccagc
    25621 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
    25681 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
    25741 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
    25801 aacaggatcc ccagcaaacc tgtctgggca attacagtca aatcaatggt attgcctagt
    25861 gcgaaggcaa agaatggaat cagcgtctgc accgctttgc tgaaaaattc tcgtaattca
    25921 gggtcaaggt tcccaagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
    25981 tgcggttcaa acgaggcaat cccggcagtg cccagaataa tcatcgtcat gagcggcccg
    26041 gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    26101 atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc cagcgttgaa
    26161 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
    26221 gcaaccaccc acgcgacggc aattttagtt accaccagcg taccggattt acgcagtacc
    26281 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    26341 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    26401 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    26461 ttctcaatca agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    26521 tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
    26581 caataaaagt gatcaaaacc acatcaactg aaacgctgtt ttatttttat cggatcattg
    26641 tttttatttt gaaattgatc acaaaaaaac caccgccatt ggcgatggtt cattttttaa
    26701 gctgatatgc ggcgtaaacg cctcactgca gcagacggca aatgattatt ttttcgccgc
    26761 gaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttga tgtagtccgg
    26821 gcggcggttc tggaatttca ggtagtaagc atgctcccac acatccaggc ccagaatcgg
    26881 gaagccggat gcgccagaaa tagcttcacc catcagcggg gagtcctggt tagcagtaga
    26941 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    27001 agctgccgct ttctcaaatt ctgctttgaa gttatctacg gaaccgaaat cacgttcgat
    27061 agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct
    27121 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    27181 gtccagtttg gtgatcaact cttcaaccgg caggttggca aattctggca ggctttccaa
    27241 cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    27301 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    27361 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    27421 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
    27481 gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat
    27541 tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
    27601 actcatctcg tcggagatgt gacgcgacga aaaatgatga ggataagaag atgagtaacg
    27661 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    27721 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg
    27781 tttcgtggat tattctgccg taggccatca gcgccctgtt actaccgaat ttctgggcgt
    27841 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    27901 ggatcggtaa tatcaactac ggcctgacca tgcgttatct cggcatgtcg atgggaattg
    27961 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    28021 atttcgatgt gttgattaac accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    28081 cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc aagatgggca
    28141 ttaaagccga agagttcaat ctgaaaaaag gactggtgct ggcggtgatg tgcggcattt
    28201 tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
    28261 cactaggcgt cgatccactg tatgtcgctc tgccaagcta tgttgtcatc atgggcggcg
    28321 gcgcgatcat caacctcggt ttctgtttta ttcgtctggc aaaagtgaag gatttatcgc
    28381 taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcagcac
    28441 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    28501 cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta ttgtgcggcg
    28561 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg acgccgtccg gtaacggtgt
    28621 tgagcctcgg ttgtgtggtg attattgttg ccgccaacat cgttggcatg ggcatggcga
    28681 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    28741 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    28801 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
    28861 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    28921 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccaacc
    28981 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    29041 aacgatggcg attcagcaac atcaccaact gcccgaacag caactctgcc atttcgttag
    29101 caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgtg
    29161 ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
    29221 tcgccccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
    29281 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    29341 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg cagaccatta ccgcgccaga
    29401 caatcaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc ggataacggt
    29461 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    29521 gatgcgccac cgtggctacc tcggccagag aacgaaattg attattcgca atatggcgta
    29581 caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg acgaatatca
    29641 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    29701 ccgcaatgat aggcgatgtc agtaacgctg gcctcgctgt gacgtagcag atgtcgggct
    29761 ttcatcagtc gcaggcgatt caggtatcgc tgaggcgtca gtcccgtttg ctgcttaagc
    29821 tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc ccaattcacc
    29881 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    29941 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
    30001 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
    30061 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg atactgccca
    30121 tccagctctt gtggcagcaa ctgattcagt ccggcgagaa actgaaatcg atccggcgag
    30181 cgatacagca cattagtcag acacagatta tcggtatgtt catacagatg ccgatcatgg
    30241 tcgcgcacaa aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
    30301 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    30361 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    30421 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    30481 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    30541 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca
    30601 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    30661 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    30721 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    30781 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
    30841 taacaatggg ctgcatagtc agaacggcta tgtcacctgg gatgtggata gccttgaaag
    30901 tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg atagcattgg
    30961 gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc gtgtgggcct
    31021 gcccgttgct tatcgcgata gccgcaccaa tggcctaatg gcgcaggcac aacaacaact
    31081 cggcaaacgc gatatttatc aacgtagcgg catccagttt ctgcccttca atacgcttta
    31141 tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg ctcacgctct
    31201 gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg aatataccaa
    31261 cgccacgacc acgcaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
    31321 gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcatagg
    31381 tcactggatt tgcccgcagg gtaatgagat tccagtggtc gccgttgcca gccatgatac
    31441 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgt gctgcttatc tctcttctgg
    31501 cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcactggc
    31561 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    31621 gggcttatgg ctgcttcagc gagtgcttca ggagcggcaa atcaacgatc ttccggcgct
    31681 tatctccgcg acacaggcac ttccggcttg ccgcttcatt atcaatccca atgacgatcg
    31741 ctttattaat cctgagacga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    31801 accgatcccg gaaagtgatg ctgaactggc gcgctgcatt ttcgacagtc tggcgctgct
    31861 gtatgccgat gtgttgcatg agctggcgca gctgcgcggt gaagatttct cgcaactgca
    31921 tattgtcggc ggaggctgcc agaacacgct gctcaaccag ctatgcgccg atgcctgcgg
    31981 tattcgggtg atcgccgggc ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt
    32041 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc
    32101 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcggat
    32161 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
    32221 gagctggcga aacagcgttt cgcggcagta gggattgatg tcgaggaggc gctgcgccaa
    32281 cttgatcgtt tacccgtttc aatgcactgc tggcagggtg atgatgtttc cggttttgaa
    32341 aacccggaag gttcgctgac cggagggatt caggctactg gcaattatcc gggcaaagcg
    32401 cgtaatgcca gtgagctacg tgccgatctg gaacaggcta tgcggctgat tccggggcca
    32461 aaacggctta atttacatgc catctatctg gaatcagaca cgccagtctc gcgcgaccag
    32521 atcaaacctg agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    32581 gattttaacc cctcctgttt ctcgcatccg ctaagcgccg atggctttac gctttcccat
    32641 gccgacgaca gcattcgcca gttctggatt gatcactgca aggccagccg ccgcgtttcg
    32701 gcctattttg gtgagcaact cggcacaccg tcggtgatga acatctggat cccggatggc
    32761 atgaaagata tcaccgttga ccgtctcgct ccgcgccagc gtctgctggc agctctggat
    32821 gaggtgatca gcgagaagct ggatccggcg caccatatcg acgccgttga gagcaaattg
    32881 tttggcattg gcgcagagag ctacacggtt ggctccaatg agttttacat ggggtatgcc
    32941 accagccgcc agactgcgct gtgcctggac gccgggcatt tccacccgac tgaagtgatt
    33001 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagccgt
    33061 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcgattgcc
    33121 agtgagattg ttcgtcacga tctgtttgac cgggtgcata tcggcctcga cttctttgat
    33181 gcctctatca accgtattgc tgcgtggatc attggtacac gcaatatgaa aaaagccctg
    33241 ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc tggaagcggc gggcgattac
    33301 actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa
    33361 atgtattgcc aacgtcacga tacgccagca ggtagcgaat ggctggagag cgtgcgggca
    33421 tatgagaaag aaactttgag tcgccgcggg taaacactgc cggatgcggc acaagtgcct
    33481 tatccggcct acaggccggc aatagttgta ggcctgataa gacgcgacag cgtcgcatca
    33541 ggcattgatt gccggatgcg gcacgagcgc cttatccggc ctacgggtcg tgcatctgac
    33601 aacaccgaat ttacaggaac acagaacatg caaaacatta cccagtcctg gtttgtccag
    33661 ggaatgatca aagccaccac cgatgcctgg ctgaaaggct gggacgagcg caacggcggc
    33721 aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt atcacgacaa cttccaccca
    33781 caaccgcgct atattccgct cagccaaccc atgcctttac tggcaaatac gccgtttatt
    33841 gtcaccggct ccggcaaatt cttccgtaac gtccagctcg atcctgcagc taacttaggc
    33901 gtcgtaaaag tcgacagcga tggcgcgggc taccatattc tctgggggtt aaccaacgaa
    33961 gccgttccaa cttctgaact tccggctcac tttctctccc actgcgagcg cattaaagcc
    34021 accaacggta aagatcgggt gatcatgcac tgccacgcca ccaacctgat cgccctcacc
    34081 tatgtacttg aaaacgacac cgcggtcttc actcgccaac tgtgggaagg cagcaccgag
    34141 tgtctggtgg tattcccgga tggcgttggc attctgccgt ggatggtacc cggcacggac
    34201 gaaatcggcc aggcaactgc ccaagagatg caaaaacatt cgctggtgtt gtggcccttc
    34261 cacggtgtgt tcggttccgg accgacgctg gatgaaacct tcggtttaat cgacaccgca
    34321 gaaaaatcag cggaagtgtt agtgaaggtg tattcgatgg gcggcatgaa acagactatc
    34381 agccgtgaag agttgatagc gctcggccag cgtttcggcg ttacaccact cgccagtgcg
    34441 ctggcgctgt aaggagcaaa catgatccgc aaagcctttg tcatgcaggt aaaccccgac
    34501 gcccacgaag agtatcagcg tcggcataat cccatctggc cggaactgga agcagtgttg
    34561 aaatctcacg gtgcgcataa ctacgccatt tacctcgaca aagcgcgtaa tctgctgttt
    34621 gccacggtag agattgaatc tgaagaacgg tggaatgcgg tagccagcac tgaaatctgc
    34681 caacgttggt ggaaatatat gaccgatgtt atgcccgcta acccggataa cagcccggtg
    34741 agtagcgagc tgcaagaagt gttttacctg ccttaatgag ttgcggtgcc ggaaaatatc
    34801 cagcaccgcg acttcatcag aagaagtatt taaagcgaac gcgtgcgccg taacggtcat
    34861 cagtatcatc accgtgtttt ttatcgccgt cgctatccag aatggagtaa tacgtcccga
    34921 ggtaaatatt aaagttctcc atatccatca cgttagcgaa ctgataagac gcatgaatgg
    34981 tgtgaatggt gtacgtccct tcatcatcaa tccagcagtc gttatcacaa actacaccgc
    35041 tgaattcatc aatcttattg tgcgcgtaga tataaccgag ctcgaaacgt ttccacagcg
    35101 cgttaatccc ggcagtgaaa tctttctcat tattagcatc taaataggcg gtatttagat
    35161 taaccacaat gccattgtcc ggatccgttt tcaggccgtt ccaggtcatg ctcataccat
    35221 agccggtacg atcggactga tcgacaaaat tacccttgct atcggtataa ccataagcat
    35281 tatttaccac gttcgcttcc atcgctgcgg aaacggtgaa ttcttccgtc ggcgaccagg
    35341 caattactgg gcgcagataa gcaacatttt tctgctgctc catatcgcgt ccatgataat
    35401 taccgtcatt atataaagat gttccgtctt caagtaacgt gtttaattca aaataccagt
    35461 tatcgagttg tttgctgacg aggaaattac cgccagcgtt agagcgcccg cggccctctt
    35521 tcatcatata gatataaccg ctgccatcgt cataaagatc gttcgcagta ttaccggaat
    35581 gttcaacaaa ggtatcctga ttcaatggga acatatcgta ggcttcaaaa cggcctactt
    35641 tgaccttcca gtcgttttct ttgccaaaga agaataccgc atcatcgata tttactgaac
    35701 cgtgcatatc acccagcggt tgtgcggaga acccggcgaa ataaccgttg tccatctttc
    35761 gcataccgtc aaaacctaac agaatacgac cattgagatt ccactgttca ttagtgggat
    35821 cgctattcac atcagcgttg gtcattgcca gcaggccatg attactttcc gcatccatat
    35881 taaattctac gtcgccgtag atttttaaat cgccataacc tgaaagcaaa agattaggtg
    35941 aggtggcatt tttctttgca gattccgccg ttgcctgtcc atttaccggc gtattgccct
    36001 gagcggcctt aatttcgcgg atctcagcgg cttgttgtgt ctgcagctgt ttaacctggc
    36061 tctctgcttt tgccgcccgc atttctgccg cttccaggcg ttcttccagc aatgccatgc
    36121 gttgttccag cattaaatgc tgtgtttttg ccataccggt actacatgcc aataataaac
    36181 cgatggctaa agataatgtc gattttttca ttatttacgt tccctgaaat aaaggagacc
    36241 ctacgctttc caaaaggaat acgtatatag agaaatataa ggtgtgcact ttcgaatttt
    36301 taatccaact tcagcaggcc gatggatagt atcccgccgc ataatatgag taaatccgct
    36361 gatttcctgg aacgtgagga atataacaat a
//
LOCUS       DAZGXS010000031        35824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGXS010000031 DAZGXS010000000
VERSION     DAZGXS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(136..276)
                     /gene="yjjY"
                     /locus_tag="ACHKG5_001720"
     CDS             complement(136..276)
                     /gene="yjjY"
                     /locus_tag="ACHKG5_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0867841.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            372..1088
                     /gene="arcA"
                     /locus_tag="ACHKG5_001721"
     CDS             372..1088
                     /gene="arcA"
                     /locus_tag="ACHKG5_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0867842.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1148..2500)
                     /gene="creD"
                     /locus_tag="ACHKG5_001722"
     CDS             complement(1148..2500)
                     /gene="creD"
                     /locus_tag="ACHKG5_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0867843.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALAIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2558..3982)
                     /gene="creC"
                     /locus_tag="ACHKG5_001723"
     CDS             complement(2558..3982)
                     /gene="creC"
                     /locus_tag="ACHKG5_001723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0867844.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3982..4671)
                     /gene="creB"
                     /locus_tag="ACHKG5_001724"
     CDS             complement(3982..4671)
                     /gene="creB"
                     /locus_tag="ACHKG5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0867845.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(4684..5157)
                     /gene="creA"
                     /locus_tag="ACHKG5_001725"
     CDS             complement(4684..5157)
                     /gene="creA"
                     /locus_tag="ACHKG5_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0867846.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5368..6237
                     /gene="robA"
                     /locus_tag="ACHKG5_001726"
     CDS             5368..6237
                     /gene="robA"
                     /locus_tag="ACHKG5_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0867847.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6234..6881)
                     /gene="gpmB"
                     /locus_tag="ACHKG5_001727"
     CDS             complement(6234..6881)
                     /gene="gpmB"
                     /locus_tag="ACHKG5_001727"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0867848.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6933..7445
                     /gene="yjjX"
                     /locus_tag="ACHKG5_001728"
     CDS             6933..7445
                     /gene="yjjX"
                     /locus_tag="ACHKG5_001728"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0867849.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7592..7918)
                     /gene="trpR"
                     /locus_tag="ACHKG5_001729"
     CDS             complement(7592..7918)
                     /gene="trpR"
                     /locus_tag="ACHKG5_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0867850.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8008..9945)
                     /gene="sltY"
                     /locus_tag="ACHKG5_001730"
     CDS             complement(8008..9945)
                     /gene="sltY"
                     /locus_tag="ACHKG5_001730"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0867851.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGNKPTLMSATEWGRRY"
     gene            10156..11823
                     /gene="ettA"
                     /locus_tag="ACHKG5_001731"
     CDS             10156..11823
                     /gene="ettA"
                     /locus_tag="ACHKG5_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0867852.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11930..12097)
                     /gene="nadS"
                     /locus_tag="ACHKG5_001732"
     CDS             complement(11930..12097)
                     /gene="nadS"
                     /locus_tag="ACHKG5_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0867853.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12131..13363)
                     /gene="nadR"
                     /locus_tag="ACHKG5_001733"
     CDS             complement(12131..13363)
                     /gene="nadR"
                     /locus_tag="ACHKG5_001733"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0867854.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13384..14766)
                     /gene="radA"
                     /locus_tag="ACHKG5_001734"
     CDS             complement(13384..14766)
                     /gene="radA"
                     /locus_tag="ACHKG5_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0867855.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14815..15783)
                     /gene="serB"
                     /locus_tag="ACHKG5_001735"
     CDS             complement(14815..15783)
                     /gene="serB"
                     /locus_tag="ACHKG5_001735"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0867856.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15889..16533
                     /locus_tag="ACHKG5_001736"
     CDS             15889..16533
                     /locus_tag="ACHKG5_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0867857.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16561..17577
                     /gene="lplA"
                     /locus_tag="ACHKG5_001737"
     CDS             16561..17577
                     /gene="lplA"
                     /locus_tag="ACHKG5_001737"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0867858.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17609..17872
                     /locus_tag="ACHKG5_001738"
     CDS             17609..17872
                     /locus_tag="ACHKG5_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0867859.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(18033..18752)
                     /gene="deoD"
                     /locus_tag="ACHKG5_001739"
     CDS             complement(18033..18752)
                     /gene="deoD"
                     /locus_tag="ACHKG5_001739"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0867860.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18809..20032)
                     /gene="deoB"
                     /locus_tag="ACHKG5_001740"
     CDS             complement(18809..20032)
                     /gene="deoB"
                     /locus_tag="ACHKG5_001740"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0867861.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20084..21406)
                     /gene="deoA"
                     /locus_tag="ACHKG5_001741"
     CDS             complement(20084..21406)
                     /gene="deoA"
                     /locus_tag="ACHKG5_001741"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0867862.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21533..22312)
                     /gene="deoC"
                     /locus_tag="ACHKG5_001742"
     CDS             complement(21533..22312)
                     /gene="deoC"
                     /locus_tag="ACHKG5_001742"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0867863.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22570..24120
                     /locus_tag="ACHKG5_001743"
                     /pseudo
     CDS             22570..24120
                     /locus_tag="ACHKG5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            24092..24955
                     /locus_tag="ACHKG5_001744"
     CDS             24092..24955
                     /locus_tag="ACHKG5_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0867864.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALLTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(25169..25951)
                     /locus_tag="ACHKG5_001745"
     CDS             complement(25169..25951)
                     /locus_tag="ACHKG5_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0867865.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(25948..27021)
                     /locus_tag="ACHKG5_001746"
     CDS             complement(25948..27021)
                     /locus_tag="ACHKG5_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0867866.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27143..27304)
                     /locus_tag="ACHKG5_001747"
     CDS             complement(27143..27304)
                     /locus_tag="ACHKG5_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0867867.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27431..28036)
                     /gene="osmY"
                     /locus_tag="ACHKG5_001748"
     CDS             complement(27431..28036)
                     /gene="osmY"
                     /locus_tag="ACHKG5_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0867868.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28429..30018)
                     /gene="prfC"
                     /locus_tag="ACHKG5_001749"
     CDS             complement(28429..30018)
                     /gene="prfC"
                     /locus_tag="ACHKG5_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0867869.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30109..30786)
                     /gene="yjjG"
                     /locus_tag="ACHKG5_001750"
     CDS             complement(30109..30786)
                     /gene="yjjG"
                     /locus_tag="ACHKG5_001750"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0867870.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30801..31247)
                     /gene="rimI"
                     /locus_tag="ACHKG5_001751"
     CDS             complement(30801..31247)
                     /gene="rimI"
                     /locus_tag="ACHKG5_001751"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0867871.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31216..31629)
                     /gene="holD"
                     /locus_tag="ACHKG5_001752"
     CDS             complement(31216..31629)
                     /gene="holD"
                     /locus_tag="ACHKG5_001752"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0867872.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31732..32763
                     /gene="rsmC"
                     /locus_tag="ACHKG5_001753"
     CDS             31732..32763
                     /gene="rsmC"
                     /locus_tag="ACHKG5_001753"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0867873.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSGVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32953..33039
                     /locus_tag="ACHKG5_001754"
     tRNA            32953..33039
                     /locus_tag="ACHKG5_001754"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32987..32989,aa:Leu,seq:cag)
     gene            complement(33078..33314)
                     /locus_tag="ACHKG5_001755"
     CDS             complement(33078..33314)
                     /locus_tag="ACHKG5_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0867874.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            33455..34243
                     /gene="fhuF"
                     /locus_tag="ACHKG5_001756"
     CDS             33455..34243
                     /gene="fhuF"
                     /locus_tag="ACHKG5_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0867875.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34281..34958)
                     /gene="bglJ"
                     /locus_tag="ACHKG5_001757"
     CDS             complement(34281..34958)
                     /gene="bglJ"
                     /locus_tag="ACHKG5_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0867876.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(34916..35641)
                     /locus_tag="ACHKG5_001758"
     CDS             complement(34916..35641)
                     /locus_tag="ACHKG5_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0867877.1"
                     /translation="MLPGCCKNGIVISKILVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8515 a         9780 c         9058 g         8471 t
ORIGIN      
        1 tggattatta aaataatgtg acgaaagcta gcatttagat acgatgattt catcaaactg
       61 ttaacgtgct acaattgaac ttgatatatg tcaacgaagc gtagttttat tgggtgtccg
      121 gcccctctta gcctgttatg ttgctgttaa aatggttagg atgacagccg tttttgacac
      181 tgtcgggtcc tgagggaaag tacccacgac caagctaatg atgttgttga cgttgatgga
      241 aagtgcatca agaacgcaat tacgtacttt agtcatgtta cgccgatcat gttaatttgc
      301 agcatgcatc aggcaggtca gggacttttg tacttcctgt ttcgatttag ttggcaattt
      361 aggtagcaaa catgcagacc ccgcacattc ttatcgttga agacgagttg gtaacacgca
      421 acacgttgaa aagtattttc gaagcggaag gctatgatgt tttcgaagcg acagatggcg
      481 cggaaatgca tcagatcctc tctgaatatg acatcaacct ggtgatcatg gatatcaatc
      541 tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt
      601 tgatgttcct gactggccgt gacaacgaag tcgataaaat tctcggcctc gaaatcggtg
      661 cagatgacta catcaccaaa ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc
      721 tgctgtcccg taccatgaat ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct
      781 acaagttcaa tggttgggaa ctggacatca acagccgttc tttgatcggc cctgatggcg
      841 agcagtacaa gctgccgcgc agtgagttcc gcgccatgct tcacttctgt gaaaacccag
      901 gcaaaattca gtcccgtgct gaactgctga agaaaatgac cggccgtgag ctgaaaccgc
      961 acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa acatttcgaa tctacgccgg
     1021 atacgccgga aatcatcgcc accattcacg gtgaaggtta tcgcttctgc ggtgatctgg
     1081 aagattaatc ggctttacca ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt
     1141 ttcaacctta tttccagata cgtaattcat cgtccgttgt aacttcttta ctggctttca
     1201 ttttcggcag tgaaaacgca taccagtcga tattacgagt cacaaacatc atgccggcca
     1261 gcgccaccac cagcacactg gttcccaaca acagcgcgct atcggcagag ttgaggagtc
     1321 cccacatcac accatccagc aacaacagcg cgagggtaaa caacatgctg ttgcgccaac
     1381 ctttcaatac cgcctgcaaa taaataccgt tcattaacgc cccaatcaga ctggcgatta
     1441 tccatgccac ggtaaaaccg gtatgttcag aaagcgccag caagagcaaa taaaacatca
     1501 ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt
     1561 caaaaacaaa gaacgccata aaagtcagcg caatcagcag aatggcatac ttagtcgccc
     1621 ggtcagttaa ttggtattga tcggcgggcg tcgtcactgc gacgctaaac gcggggaagt
     1681 tttcccagcc ggtatcattg cctgaagcaa aacgctcacc gagattatta gcaaaccagc
     1741 tgctttgcca ttgcgcctga aaacctgact cactaacttc ccgtttggct ggtagaaaat
     1801 cacctaaaaa actgggatgc ggccagttgc tggttaaggt catttcgcta ttacgccccg
     1861 caggcaccac agaaagatcg ccggtaccgc ttaaattcag tgccatattc agcttcaggt
     1921 tctgcttccg ccagtcccct tcgggtaaag ggatatgcac gccctgcccg ccttgctcta
     1981 acccgatgcc gggttcaata gccagcgccg ttccgttaac ttcaggcgct ttcaccacac
     2041 caataccacg cgcatccccg acgctaatca caataaatgg cttgccaaag gtgatatttg
     2101 gcgcgtcgag ttcgctaaga cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt
     2161 gccagacctg accggtataa atccctatct tgcgttcttc cacgttctga ttgccatcaa
     2221 ccatcaatga ctcaggtaac caaaaatgga taaaacttcg tttccgctcc acggttttat
     2281 cctcttcctg caccgtataa agctcggtca caggaatagc gataagaggc ccaacgagtt
     2341 tttgcggccc gctggtactt tggcgaatcg catcttccac atcgctacgg taatcggcac
     2401 gttcgacaat cacctgccga atcagcatta tcggaatcaa caacagcaat actgcaccaa
     2461 acagggtggt cattttccag aacagggggg atttcaacat agtcttctcc tttgcaatgg
     2521 cagcaggata cgaaggctat gtgggaagaa tttgaagcta tgtgaagtga cggtgaagcc
     2581 gaagcgaggc cagcacgcca ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca
     2641 aacgggcgac ctcactgaca aacgccaacc ccagaccgct gcttttttgc ccatttgcac
     2701 gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc gtagtcagga ataccactac
     2761 cggtatccag caccttaagg gcgacgtgtt cctgatccac ttcggcgctt agcgttatgc
     2821 gaccgctctt gggggtaaaa tcgatggcgt tatccagtaa attccccagc gcctgctcca
     2881 gtaacgccgg ttcagcagca acgttaacct cagtaggcat aacatgcaaa gtgatgtttt
     2941 tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa
     3001 cagcagtcag aacgacttcc tgacgattct ccagtcttgc ctggcgtagt aacgtttcta
     3061 ccaatgcctg catacgcgca ttttgcgtca gaatgttgtc ggtaaaacga gccaccactt
     3121 ccggtggcgg accttcgcgt aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt
     3181 ttagctcatg ggttaacgca taaacatact gctcaatata gtttttccct tccagcttca
     3241 cgcgcatact ttccagcgcc tgcgcaagct tacgcaactc gctactgccg agttcaggaa
     3301 gaggaacggg cttattgtca gtgacggaat cggcatagcg agtgagccgg gcaatggagc
     3361 ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc
     3421 tggcccataa aattcgccgc tcgctgcgct taatgaccgg agccatcgcc gcgttcggtt
     3481 tgcctacgct caaaacgcca ataagccgcg agccgtccat aatcggcgcg gcaacataca
     3541 tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct gcgcgcacca tactgaccac
     3601 gcaacgttag ccagacgtca ttccagcgcg aataatcctg cccaacggct ttatttgccg
     3661 aatcgaacaa tactttgccc tgcgcatcgg tcatatagac atgatattca ttgcgcactt
     3721 tgttaatgcc accgatatta gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg
     3781 ccagttgccc atgcgttggg tccccagaga gcaaatcggg acgcgccagc tccgccagca
     3841 acgttgcggt gtcgatcagc gtcccctccg ttgctcttcg cacgcccggt ttaacttctt
     3901 tgacaaaaat agccagtacg aaccaggccg ccaccgccac cagtaaaaaa tagcccagca
     3961 acaaccgcat gccgatacgc attacaggct cctcaggctg tatcccatgc cgcgatgagt
     4021 attaatcggt gaaagatcgg ggttgatggc gcgcagcttg gcacgcagcg ttttaatgtg
     4081 ggtatcgacg gtgcgatcgt aggtgtcctg cgcatcttcc cacacgatat ccatcagttg
     4141 ctggcgggac cagacgcggc ctggcgactt gagtaacgtc ttcagcaata aaaactcata
     4201 ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc gccgcgggtt cattcaattc
     4261 aaaatgacca atacggatga cgggagacgg cgtcgagaac ttcttcaccc gacgcagcaa
     4321 ggtgcgcacc ctagcgcaca cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc
     4381 gccaatttcc agcccaagca ggcgatcgac ctcttcactt cgggccgtca ggaacagtac
     4441 aggtaacgcc ggatggagcg ccagtaactg gcggcacaat tcaaagccac tgatatcagg
     4501 caaaccaacg tcgagaatca tgacatcggg aacctgctgg cgagctttat ccagcaccgg
     4561 caagcctcgc tcaaagacct cgacggcaaa accttcctgc tgcaacatgt agaccagcgt
     4621 gtcggctatc ccttgctcat cttccactaa ccagaccgtt tcccgttgca taaatcgcct
     4681 ctgttattgt cgccacggca tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc
     4741 tacaactttg tcggagtaag ccagataagc gagcgcgttg cgtttggcgt cataaaagcg
     4801 cacgacctgt aacgacttaa agaccaggga cgtgcgtttt ttgaatacta cctcgccctg
     4861 agctttgccg tttttaatac gatccgacag ttcaatcggc ccgacttgct gacaagaaat
     4921 agccgcatcg gaggtatctt ccgccagacc caatccccct ttaataccac ctgttttcgc
     4981 ccggctcaca taacaggtga catttttcac atcgggatcg tcaaaggctt ccacaacaat
     5041 tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg
     5101 agccaatggc cccagcatta tcaggcttaa agacaggatc aaatgcttgt atttcatatt
     5161 gttaccattt ttatctatta cacgagcaat tagttcgtca cggtaaataa catattgatt
     5221 aagaacaaaa tctcaatact tttatttccg tcaagaccta aaacatactc tactaagaaa
     5281 aaaacactga atgctaaaac agcaaaaaat gctattatcc aattacctga tgtcaggtgc
     5341 tcgttgttga aaggatgagg atattttatg gatcaggccg gcattattcg cgacctttta
     5401 atctggctgg aaggtcatct ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca
     5461 ggttattcca agtggcactt acagagaatg tttaaagatg tcactggcca tgctattggc
     5521 gcgtatattc gtgctcgtcg tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt
     5581 ccgattctgg acatcgcgct gcaataccgc ttcgactctc aacagacatt tacccgcgca
     5641 ttcaagaagc agtttgccca gactcctgca ctttaccgcc gttctcctga atggagcgcc
     5701 tttggtattc gcccgccgct gcgtctgggt gaattcacta tgccagagca taaatttgtc
     5761 accctggaag atacgccgct gattggtgtt acccagagct actcctgttc gctggagcaa
     5821 atctctgatt tccgccatga aatgcgttat cagttctggc acgattttct cggcaacgcg
     5881 ccgaccattc cgccagtgct ctacggcctg aatgaaacgc gtccgagtca ggataaagac
     5941 gacgaacaag aggtattcta taccaccgcg ttagcccagg atcaggcaga tggctatgta
     6001 ctgacggggc atccggtgat gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt
     6061 ctgggaaccg gcgtgcagga gtttatcctg acggtatacg gaacgtgcat gccaatgctc
     6121 aacctgacgc gccgtaaagg tcaggatatt gagcgatact acccggcaga agacgccaaa
     6181 gcgggagatc gcccaattaa tctgcgctgt gaactgctga ttcctatccg ccgttaacgc
     6241 tgcagctcat ctaatgcagg ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc
     6301 cagccagacg ccagccacag gctttcctga taatccacgc gcgaaataga acagttacgc
     6361 agacgtaagc ggcgctctgc ccatgctggt aatccgagaa tcgtactcac cagacatccc
     6421 agtgcaatac catggctcac cagcagcggt cggcttccct gcggtaagtc ccggcaagat
     6481 tccagtgccg cattgacgcg atcgctgagc tcctgcattg actcgccttc aggaatacgc
     6541 ccatcaacgg tgccattgac cagctgccga cgccagttct cttcttcttc ggtcagagaa
     6601 tcgatatgtc ttttttccag cacacccatg tttaattcac gcaggcgaga atcaaagatg
     6661 atgtcacagc cgcaggcctg ggcgatgatt tccgccgtac gccgggtgcg tcctaaatcg
     6721 ctactgatga tatgcgtaat gccaagctct ttggcacgcg tcgccacctg catcgcctgt
     6781 tgctcacctt tggcggtcag cgggctgtca gactggccct gaatacgtcg ctcggcgttc
     6841 cactgcgttt caccgtggcg gactaggtat acctgtaaca tgcttttttt ccgtatactg
     6901 ctgtaacgtt aattttctga gtaatgctga ttatgcacca agttgtctgt gcgaccacca
     6961 atcccgctaa aattcaggcc attctgcagg catttcacga gatcttcggc gaaggatcct
     7021 gccatattgc atccattgcc gtcgagagcg gtgtaccgga acagcccttt ggcagtgagg
     7081 aaacgcgcgc tggcgcacga aaccgggtag ccaatgcccg ccgtttactt ccagaggctg
     7141 atttttgggt ggcgattgaa gctggcattg atggcgacag cactttcagt tgggtggtga
     7201 ttgaaaacac cacccagcgc ggcgaggcgc gttcggcaac tttgccgttg cctgcggtga
     7261 tattggaaaa agtacgtgaa ggtgaggcgc ttggtcccgt gatgtcgcgt tataccggta
     7321 ttgatgagat tggtcgtaag gaaggggcga tcggcgtgtt taccgccggg aaactcactc
     7381 gcgccagcgt ctatcaccag gcggtgatcc tggcgctgag tccgtttcat aatgcggtgt
     7441 attaagcgcc tgatgcgacg ctgccgcgtc ttatcatgcc taccaaacat attgaaatta
     7501 cgggtatttg taggacggat aaggcgttca cgccgcatcc ggcattcggt gcacgatgcc
     7561 tgatgcgcca cgtcttatca ggcctacaaa atcaatcgct tttcagcaac acctcttcca
     7621 gccactggcg cagctccacg ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg
     7681 cgatgcctgc gccgagttca ttttttaact cacgctggct catttcgccg cgcaacagct
     7741 cttcgacaat acgcacgcga gtccccaacg cttcgcgctc atctggcgtc agcatcaggt
     7801 ttaacaacgg taaatggaga tcgttttggt aggcattctt aagcaggtcg acaaaacgta
     7861 accactcctg gtgacgctgt tctgccatcg ctgctgaata gggtgattgt tgggccataa
     7921 tatgtcgcca ttgttagcgg gggaagcaaa atgcctcccc cggttgtact cgctaaagag
     7981 tacgatagca tatcataaac gtgcggatca gtaacgacgt ccccattccg tggcgctcat
     8041 caacgtcggt ttattcccca tgaaatagcg gtagtaagcg tcataagcca gcacgttctt
     8101 cacataaccg cgcgtctcgg agaacggaat actctcgaca aatgccacag cgtcgatacg
     8161 cccggcgctg ttgccaagcc aggttcgcac ccgtccaggt ccggcgttat aagctgctga
     8221 ggagaaaata cggttattac caaattgctg ataaacatat tgcaggtaac tggtgccaat
     8281 gttgatattc gtttccggat ccagcaattg cccagggctg ctataaccag gaatagagaa
     8341 catcttcacc gtatgggtcg ccgtgcccgg cataatctgc atcaggccgc tggcccctac
     8401 cggtgatttc actttcggat tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct
     8461 ttgcgggatc tccttcccac tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa
     8521 tcgctcttcc agatgatccc aaagcttccc ggcgatcgtt gcctgaacgc taagatccca
     8581 ccattggttg ttgaaagcat accgcgccag ttgagcctgc tctgtttttg acttgctctt
     8641 caccagattg gcccactcgc tacgcgcggt gttatcgaga ttccagtaca tcaactcgcg
     8701 cacgcgcgcc atctccggcc cctgagtcag ggcgctgtca acattctgcg gcgctttatc
     8761 aatcttcagc tcatactctt cgccgatgcg ttgtgcagca accatcgggt agaaaccacg
     8821 ctgttgcatg agttgatgca aaatctcttt tgcttcagct tcacgtccgc gttccagcaa
     8881 taaatccgcc tgccagtaac gccattcatc tttctctttc gcttccatag gcagacgcgc
     8941 cagccaggta ttcaggccgc gacgatcgcc agtgccaagc gccattcgca cacggcgttc
     9001 aataagcgaa gtagattgcg agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc
     9061 ggtgacatcg ttgcccatca aacgccaggc gacgatatcg cgcagctcct gaatctgatc
     9121 ttcattaagc tgctgcgcct gggcaagcga tgggatcatc agccgtgcat tttcagcatc
     9181 ctgccgcgcc acactggcaa acgccaccgc cgccatttga cgggtaaaat cggtcgctcc
     9241 ggttgtacgc gcgaaggtca gtaccgtatt ggggttgttc gccagtgaaa tgattgccga
     9301 ggcgatagtc tggtaatcgg caggcatctg ccctgccagc actgttacca ggccagtgtt
     9361 acccgctttc atcgccagac ggatacgctc taaatacgcc agcggatctt gtttacctga
     9421 cgcacgccag acgctaaata acttgtcaca ggcgttaggc tggctcttgc cggttagcca
     9481 cagctctttc gccccttgcc aggcttcttc actctgcccg gtgttccatt tcgcatagta
     9541 gtaattacat tgtgcttcgg tagttccggg cttttccggg ctaaaggcta acaggccacg
     9601 ccagtcttca cgccgcgcca gttcattgac gaaacgagat tgcagcgtgc gagcgggagg
     9661 aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc gccggttgat tcatcaggtc
     9721 atcggtgatc tggcggtatt ccaggtaggg ataaagcgga taatccttca gtccaggcat
     9781 catttgttcg accacatcca tttgtcgatt atcccaggcc tgcttgattt gcgcgtaacg
     9841 actacgctgc tcatccagtg agtcggctcg cgccacgctg ctgaccgtca gcagacagac
     9901 accggcagcc aacagccgcc aggtaacttg tttggctttt tccacaagcg catcctctaa
     9961 gtgtaaatac atcaatgcag cgtcatgccg cgtaatagat ttatgctaac cagtcattgc
    10021 cgtttacgcc acgttacgga cactttttta cttttactgc gagggtgatc gaccggctgg
    10081 gattaggcgg ggaaaaaggc tacacttcgc ctttgaaaac tcatcatcac gataaacata
    10141 gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt
    10201 ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt
    10261 ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt
    10321 gataaagaca tcgaaggtga agcgcgtccg cagccagaca tcaagattgg ttatctgccg
    10381 caggaaccgc agctgaaccc ggaacacacc gtacgtgagt ccattgaaga agcggtttca
    10441 gaagtggtta acgccctgaa acgcctggat gaagtgtatg cgctgtacgc cgatccggat
    10501 gccgattttg acaagctggc cgctgaacaa ggccgtctgg aagagatcat tcaggctcac
    10561 gacggtcata atctgaacgt acagctggag cgtgcggcgg atgcgctacg tctgccggac
    10621 tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc
    10681 ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac cgaccaacca cctggatgcc
    10741 gaatccgtgg cctggctgga acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt
    10801 acccacgacc gttacttcct cgataacgtt gcgggctgga tcctcgaact tgaccgcggt
    10861 gaaggtattc cgtgggaagg taactactcc tcctggctgg agcagaaaga tcagcgtctg
    10921 gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg
    10981 gtacgtcagg gaactaaagg ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa
    11041 gaactgaaca gcaccgaata tcagaaacgt aacgaaacca acgaactgtt tattccacct
    11101 ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc tgcgtaaatc ctatggcgat
    11161 cgtctgctga ttgatgacct gagcttctcg atcccgaaag gagcgatcgt cgggatcatc
    11221 ggtccgaacg gtgcgggtaa atcgaccctg ttccgtatga tctctggtca ggaacagccg
    11281 gacagcggca ccatcacttt gggtgaaacg gtgaaactgg cgtcggttga tcagttccgt
    11341 gactcaatgg ataacagcaa aaccgtttgg gaagaagttt ccggcgggct ggatatcatg
    11401 aagatcggca acaccgagat gccaagccgc gcctacgttg gccgctttaa ctttaaaggg
    11461 gttgatcagg gtaaacgcgt tggtgaactc tccggtggtg agcgcggtcg tctgcatctg
    11521 gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg acgaaccaac caacgacctg
    11581 gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg agttcccggg ctgtgcgatg
    11641 gttatctcgc acgaccgttg gttcctcgac cgtatcgcca cgcacattct ggattaccag
    11701 gatgaaggta aagttgagtt cttcgaaggt aactttaccg agtacgaaga gtacaagaaa
    11761 cgcacgctgg gcgcagacgc gctggagccg aagcgtatca agtacaagcg tattgcgaag
    11821 taatgcgtaa aatgccggat gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt
    11881 tcaatacgtt gtaaaactgt agacctgata agacgcggta agcgtcgcat caggcattga
    11941 gcaccgaatg ccggatgcgg cgtgaacacc ttttccggtc tacaacagcc ttagcatcct
    12001 caatttcgtg gcgggccacc cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct
    12061 cttccagaga ggttttcaac tcatcaaaaa aactcatttt atacctcgcc tttgagcagt
    12121 ttcatcacgg ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga
    12181 aacggctgtc gtaatcttcc tcttcaaccc gcacaaattc gatattgttc tcttccagca
    12241 tctccaccag cagattctgg aactctttac gatccaccga actgccgagg ctgcgtaaac
    12301 catccaccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat
    12361 caatcagcgc ctgcacgaac ggatgttccc gcccttcgta ctttttgcag aacgcctgag
    12421 tggtgacaaa atcggtatcg ataaatgcca ctttattggc atatttcacc gcaaaatcaa
    12481 tgtattgagc gtggcccagc gcgattttat cgtagtcaga atactgcaat gcgatctcat
    12541 caccgccgag gtgtgaaaag acataatcgc ggccatattc ccacgcactg gtggtgttga
    12601 agatattggc aagtttgttt accagggtgg atttaccgct cgactcgccg ccaaggatcg
    12661 ccacggtacg cacaaaaaac ggcttcactt cggtaggaat atattcccag tagcggaacg
    12721 ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagca
    12781 ccgtctcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga
    12841 tcagatccgg ctggatcccc ttttcagcca taaacttttt gataccgttg ctccacacat
    12901 cccagccgtg cggatacggc tccatgccct cttcgttgaa agcatgaatg cgaatatttt
    12961 tctgatattt aaaagtttgc aataaccagc gcagacgatc cggcacggtc ggctgctgcg
    13021 acatggcact gtcttcgaac aacgcgcggt cacgggtatc gtcaaaaccc ataatgatat
    13081 gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagtg
    13141 ggtagaactt accgaatacg acaccgatcg ttttcttctg ccggggaaac tcaagcccca
    13201 aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttgattttg gcattcagta
    13261 actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc
    13321 cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatttc
    13381 tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg
    13441 catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc
    13501 gtgtttcgcc gcttcagaga ttcgttcctg accgctgggc accgggcgga tctcccctgc
    13561 cagcccgact tcaccaaaca ccaccagatc ctgcggtagc ggtctgtcac gcaggctgga
    13621 aaccatcgcc agcagtaacg ctaagtcggc gctggtttcg gttaccttta cgccgccgac
    13681 cacgttcaca aacacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc
    13741 cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg gattcgccat
    13801 catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac
    13861 caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg
    13921 gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc
    13981 gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat cgccatctaa
    14041 aagcaccgaa cagtcgatac agtgttccag cactttcggg ccagccagcg aaccatcttt
    14101 ggttacgtgc cccaccatga caatcgccac accgcgcgtt ttggcgaagc gtgtcaaata
    14161 agccgccgtt tcacgcacct gcgccacgct accaggcgat gactgtacat ccgccatatg
    14221 catcacctgg atcgagtcaa ttaccatcag cttcggttgc tcttcttcgg caatcaggca
    14281 gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg
    14341 atgagcgcgc attgccacct gttgcagcga ctcttcgccg gtgacataca gcgttttcat
    14401 ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg caccagggtt
    14461 accgccaatc agaatggcac ttcctggcac cacgccgccg cctagtacgc ggtcgaactc
    14521 tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac
    14581 ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgtagg
    14641 cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc
    14701 ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt
    14761 tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga gcaattactt
    14821 ctgattcagg ctgcctgaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt
    14881 gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgccgc
    14941 tttgatcatc ggcaggtcat tggctccatc gccaatcgcc acggtctgcg ccagcgggat
    15001 ttcatactcc tgcgcgaggc gagtcagagt tttcgctttg tactgcgcgt ctacgatgtc
    15061 gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc
    15121 tgtcaggcgc agcttgtcgc gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat
    15181 cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag
    15241 cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac
    15301 gcggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc
    15361 cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat
    15421 ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggatttttcc
    15481 cagcggcgcg acatccagct gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga
    15541 acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc
    15601 cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag
    15661 cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga
    15721 aagaggcaga cccggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg
    15781 cattaaggct cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta
    15841 tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca
    15901 aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg
    15961 gcactgatgc agggggcgtc atggtttagt caaaaccacc agcgacagcg taatccacag
    16021 ctggaagaac tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc accgctgatg
    16081 cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa
    16141 agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc acgttctggc
    16201 gaaagcgtcg aagtgcgcga tcggctggcg ctcgacggta aaaaagcagg cggctatttt
    16261 aaccagcaga ttgtcgagcc aattgccggg aaaaacggac cgctcggcta tctgcgcctg
    16321 acactcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt
    16381 ttacgcctga tgttgctgct ctcactggca atcggcgtag tgctgacccg cacgctgcta
    16441 cagggtaaac gcacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg
    16501 ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt
    16561 atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg
    16621 gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat
    16681 gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg
    16741 atggaagaag ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac
    16801 gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc
    16861 tccacgtcga ttgtgctcaa tgcactgaat gcgctcggcg tcagtgccga agcgtccgga
    16921 cgtaacgatc tggtggtgaa aaccgccgaa ggcgaccgca aagtctcagg atcggcctat
    16981 cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc tgctcaatgc cgaccttagc
    17041 cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacttca
    17101 gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatccccca tgagcaggtt
    17161 tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcgcgtgga agcggaaatc
    17221 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgccagagt
    17281 agctgggagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    17341 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    17401 gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    17461 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    17521 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    17581 gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg aacgggagat
    17641 tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    17701 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaacagat
    17761 cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa tttatggttg
    17821 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc
    17881 gtgaatagat acttgaatgg attttatttt ctatccatat gaaaaataat agtaaatcta
    17941 taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc cactccggct
    18001 taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat
    18061 tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtttgc
    18121 tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc gccaaactct
    18181 gcagccacgc cgtagatacc agccgcttcc atttccacgc cgagaatgcc gtatttttcc
    18241 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttacca
    18301 acgcgagcat caatgcccag tgctttagct gcatctactg cgttacgcac catgtcgaag
    18361 tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt ggaatcggtg
    18421 caggcaccca taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag
    18481 gaacccacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg
    18541 gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta
    18601 ccggtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
    18661 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg
    18721 cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg acataacaaa
    18781 ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat tccatatcag
    18841 aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca cggtgaccca
    18901 gtgagcccgg ttttactttc gggccgtaaa ccagtaccgg aatgtgttca cgcgtgtggt
    18961 cagtaccggt ccaggtcgga tcacagccgt ggtcagcggt gaggatcagg atgtcgtcat
    19021 cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga cccgcagcat
    19081 aaccggcgac gtcgcgacgg tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga
    19141 cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca
    19201 ggccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct gcaattttac
    19261 cgaccgaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg gtcggtgctg
    19321 gcggctcaac agccaggtcg tgacggttac cggtacgctg gaagttaccg gctttgtcgc
    19381 cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc agctcttcac
    19441 gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca tggcaggcaa
    19501 tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc atgtgctctt
    19561 cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg taacccggca
    19621 gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg
    19681 agaaatatcc ccactcaaac agaaccggga caccggcaat ttcccagtga ccagacgggg
    19741 tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc
    19801 cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc agccccagac
    19861 gggtcagatt tggcagattg agcgggcctt tacgaccgtt atcagcttcg cctttggcac
    19921 aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt tctgcatctt
    19981 ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc atatgttctc
    20041 cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg ctgatacggc
    20101 gatagacagt tggtgtgctt tccggtgctt tatcggcaag tttaattgcc gctttcaccg
    20161 ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgtg gataaccgcc agcggacgct
    20221 ggccgtctac ctggtcgccc agacgtgcca tatcagtaaa gccgacgctg taatcgatgg
    20281 tgtcagatgc ctgacggcgt ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg
    20341 tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc agcatcgctg
    20401 tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt gccgctacca
    20461 tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg
    20521 cttcggcgtc atctttcgcc agtttgccgg agataagcat ctccacacac agcgccatcg
    20581 tgacatcaaa cagacgcggg ttacgatatt cacccgtcag gaactgcacc gcttcacgta
    20641 cttcaaccgc gttaccggca ctggaggcca gcacctgatt catgtcggtg agcagtgcgg
    20701 tggtgcgcac gcctgcgccg ttagccacgc caacaatcgc ttcggcaagg gcttcagaga
    20761 gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc agcgcgtcca
    20821 gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg gagtccacgg
    20881 ttgcggtaat atcacgggtc gcgtagaaac gtttatccgc cggagccagt gagctggtct
    20941 gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg tcatccggga
    21001 aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta tgaccgaggc
    21061 cgcgaccaga gatcatcgga atatagccgc cgcaggctgc gaccatcggc cccaacatca
    21121 gcgaagtcac atcgccgacg ccaccggtgg agtgtttatc aacaatcggg ccattcagat
    21181 gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc agcgagacac
    21241 gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca atctgccctt
    21301 cggagatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca tcgctcagcg
    21361 catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta acctcccgag
    21421 gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc ctacgggtag
    21481 atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt acttagtagc
    21541 tgctggcgct cttaccgtcg ccgtgaccca gcgctttcag caggcttgcc agcaggctgg
    21601 aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt tcatctgcga
    21661 tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa
    21721 cggttttttc tacgcccata tcacggatca cttccatcat gatgcgcgcg ctttccggcg
    21781 tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg
    21841 agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt tcgatgatca
    21901 ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc aggtcaaaac
    21961 caacctgctc gttacccgcc atcagcgcgc ggtacgggaa cacaacgtca acttcatcag
    22021 caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt
    22081 gtgggaagtt ggttaccgta gcaatacgga tttccggggt gccctgctct ttcagtgttt
    22141 tgcgagcaat cgggataaag cgaggataga tacagatagc ggcggtattg ccgaccggag
    22201 ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg
    22261 tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc atttcattct
    22321 cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa catctactcc
    22381 gcaacacact tcgatataca tcacaattaa ggaaatctac ttacaagttt gcatcactat
    22441 aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg gcaaacgcat
    22501 caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc ggcataaaag
    22561 gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc
    22621 accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga agcagaaaac
    22681 aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc
    22741 tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc cgattacgcc
    22801 cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga tcttgatgac
    22861 gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc gatgccggtc
    22921 tacctggggc aactggatgc gttgttgcaa ccgtatgtta taattctaac acaagacgag
    22981 atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc agacgccttt
    23041 atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt acgtgcagac
    23101 gcagatttga agcaggtttc accgaacctg acctttatct acgatcctga aatcacccct
    23161 gatgacctgc tgctggaagt ggcgaagaac atctgtgaat gtagcaaacc gcacatcgcc
    23221 aacggtccgg tgcatgataa aattttcaca aaaggggggc tacgggattg tgagctgtta
    23281 caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat
    23341 tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca
    23401 gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt
    23461 tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt
    23521 tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gaaaaagaag ggattgccgc
    23581 gcgctacggt aaagaagccg ccgcaaatga agtaggttat cgcatcagtg cacagctggc
    23641 ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc
    23701 acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga
    23761 tgagccagat ccgatcaccc atctgcaaac tgtcgcccgc atcatgctta ttattattct
    23821 ggcatcagcg acattctgac gctcgacgaa accatcaaac gtaacccgca ggcgttggta
    23881 cagctttgcc tcggtgcctt taaagccgga atgcgtgaat ttaccgccaa tgtcagcggt
    23941 aacgatctgg ttcgcgttac cggttatatg gtgcgtttgt cggatttaga aaaatatcgc
    24001 gccgaaggtt cacgcaccaa caccacctgg ctgggcgaag aagccgcacg caacactcgt
    24061 attctggaac gccagccgcg cgtgataagc catgaacagc agatgcgctt tagtcagtaa
    24121 gattatcccc ttctcctgcg ttgacgggcc aggcagtcgt ctggctctgt ttttgcaggg
    24181 ctgcaatctg cgctgcaaaa actgtcacaa tccgtggacg atgggacgtt gcaatgactg
    24241 tggggagtgc gtgccacagt gtccgcatca ggcgttgcag attgttgacg gcaaagtggt
    24301 gtggaacgct gtggtctgcg agcaatgtga tacctgcctg aagaggtgtc cgcaacatgc
    24361 cacgccaatg gcgcaatcca tgagcgtgga tgaggtgctt agccatgtcc gcaaagcagt
    24421 gctgtttatc gaagggataa cggtgagtgg cggcgaagcc acgacccaac tgccgtttgt
    24481 agtggcgctg ttaactgcta tcaaaaacga tccgcaactg cgccacctca cctgcctggt
    24541 ggacagtaac ggtatgttga gcgaaaccgg ctgggaaaaa ttgctcccgg tgtgcgacgg
    24601 cgcaatgctc gatctcaaag cgtgggggag cgaatgtcat cagcaattaa ccggacgcga
    24661 taatcagcag attaagcgca gcatctgttt gctggcagag cgcggtaagc tggcggaact
    24721 gcgtttgctg gtaatccctg gccaagtgga ttatttgcaa cacatcgaag aactggcggc
    24781 gtttatcaag ggagtcggcg atgttccggt acgcctgaac gcgtttcatg cccacggcgt
    24841 gtatggcgag gcgcaaagct gggcgagcgc cacgccggaa gacgttgagc cgttggctga
    24901 tgcgttacgg gagcgcgggg tgagccggtt aatatttccg gcgctgtatt tgtgacatgc
    24961 cggatacgac ctacggttcg gcacagactt gtaggcctga taagacgcgt caagcgtcgc
    25021 atcaggcatt gtgcgccaac tgccggatgc ggcgtgaacg ccttatccgg cctacggttc
    25081 ggcacaaacc tgtaggcatg ataagacgcg ttaagcgtcg catcaggcat tatgcgccga
    25141 ctgccggatg ctgcctacga agggcaaact acccacgaac gttaaacacc gcatacgtgt
    25201 tattaagcaa cacttcggca atctcatctt ctggttccgg gcgcaactcg caaagcacgt
    25261 caaacacccg cgcagcctgt tccgggcggt taggctgccc ctgaaaaccg ttgagcggca
    25321 tatccggcgc gtcggtttcc agcaacaacg atgccagcgg taattttgcg atgacaacgc
    25381 gggttttact ggcgcgtgga taggtgatag taccgcctac gccgattttg tagcccagct
    25441 gtacaaaccg ctcggcctgt tgcaggctgc cggaaaaacc gtgaaccacg ccagtgcgcg
    25501 ataaatcgtg gcgtttaaga tgcatcgcca gtttgtcgtg tgtgcgccgt gaatgcagga
    25561 tcaccggcag atcgtagcgt ttcgccagtt tcagttgttc gtcgagaaac cactgctgcc
    25621 tctcaaattg cggatcgtcg ccgaagagat ccagaccgct ctcccccacc gccaccacct
    25681 tcgccggacg cctttccagc gcctgctgta gctgatcaag agacacatcg ctatgttttt
    25741 ccaacatacc aggatgcaag cccaatgcgg catacagcgg ttgataattt tccgctaacg
    25801 cctgcacgcg ggcaaaattt tccgcctcgg ttgccggaac aatgatcttg cctacgcccg
    25861 cttgtgccgc gcgttgcagg ctggcctctt catcgccaat aaaaggcggg aaatcaaaat
    25921 ggcagtgggt gtcgataaaa cggcaaatca agccagatcc tcattgttaa atgtcgggtc
    25981 gttcgcctgc ggagcgtcgc tcacttcggc aaccagcgta tcgttggcga ctggcgcagg
    26041 cggaatgaca atcgattccg gcgtcaccac tggcaccaga tggcgggtaa gcggcgcttt
    26101 ttcagttaat agcttgccaa ccgtggcgag gaaataacga ccgcataaac gcccaagttt
    26161 atagtcttca cgcagcgccg gaatccgact gccaagcgcg atactatgta atggcttcgg
    26221 cggataaatt tcgaatatcc gcagcttgcc cggtggtttc tcaataaatt gctgaatgtc
    26281 acgatagctg gtttcatgat gctgcaccag attgaccagc ggctgcaggc tactgtcacc
    26341 cagccagcgt tccatgcgtt tgaaccactg cggcgtgtag tacatttgtg acggcacagt
    26401 gcggatgacg accaacgttt tagcgccctg ccttgccgcc tctttaaccg gaatcgcatc
    26461 actgatcccg ccatccaggt agttaatgcc ttccagcgac actccgctac gataaaagcc
    26521 aggtatcgca ctggaggcgc gaatcacatc cagccagttt tgtttggttg gtaaaaagta
    26581 attcggcgcg tagtcatcct gacgacaggc gcacatataa aacgatttgc cgctgtcaaa
    26641 caaccgcgcg gcggtatcca tttgcaacgg catctggctt gcggtggcct ccaccagcca
    26701 gtcgagatcg ataagatttc ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt
    26761 tgtggtatag cgcatgatga ctttgcgcgc gtaaccgggc tgattgcaga tatacgccga
    26821 gaggttctgc gccccggcag atgtgccgag ataaagatcg aaaggattaa actgcgcgcg
    26881 cataaactca tccagcacgc cagccgtgaa aattccacgc tgtccgccgc cttcgcacac
    26941 cagagctatt cgtccaggct ggaacggcct tagcgacaac ggcgcaatat taccaagcgt
    27001 tacaggtatt cgctgcccca cctctgcctt cctgtttttg ttgtgaaaga atgacaaagt
    27061 aacgcaattt attattcgct aaaaccgcaa aagccagtcc gctggactgg ctttggataa
    27121 aattttctta atggtctgaa atctagggtc gttttcggcc catgaacaaa ctcaccagga
    27181 acagaataat cccgacgaca aagacaattt tagctgcgcc tgcagcggta ccggccagac
    27241 caccaaaccc aagtgcggcg gcgattaacg cgataaccag aaatatgatg ccccaacgaa
    27301 acataagcgt ctcctttacc atagttaatg tcaccgcttg atatgagcgt gaaaatcact
    27361 caatgcgata tttttagtgt ggtgcacatc gcgcctcccg acaaagttcg ggaggacgaa
    27421 ttacgacgaa ttacttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt
    27481 tggcgatact ttcagcacgg tcactttgtg cctgagaatc tacgatacca gagagctgaa
    27541 ccacgccatc ggtggtttca actttcacat gacgggaagg gacgatatcg tccgccagca
    27601 atttggcttt gatttcactg gtggtggcgg tgtcacccgc gtagcccttc acagagcctt
    27661 ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt caccccttca acacctttcg
    27721 ccactttcac tgcctcttcg gcctgggcct ggctttcaac gaaaccgctc agggtcacga
    27781 ctttttgatc ggttttgaca gagatatcgg tgctcttgat gttgtcatga tccaccagcg
    27841 ccgccttcac tttcgcggtg atggcgctgt catccatgaa attaccgact ttattcatag
    27901 agctatcgac tttttgccct gcgctttcat tggtagtctg cgcgttgttt tccgcgtagg
    27961 cagagccggt cgcgacggca gaggtcaaca ttacagccag cagagttttc gaaatcttca
    28021 gtcttgtcat agtcatcgat ttattcctgt atgtttgctc gtaatttgag cctggcaaca
    28081 cgaggttgca ttgctgaata gggagagact tcaccctcta cagaagtcaa tggtcgccat
    28141 cacaaaagcg atgagtgatg aataacgacc attacagcct ctgaatcagt tattaatatc
    28201 ggtagaatga caatcgacgg ctttagatac tgatatctac gcattgaacg gtatttaacg
    28261 ccgtcagaaa tgtcatcact ttgttaaata tagatcacaa ttttgaaacc gctcgggata
    28321 tcagcgaaaa cataagcaaa agtgaatgtt ttaagaacat tccgtaagcg gctaataagg
    28381 aagggaaatt gacaggacgc agcggctacc gcgccctgga ggcaagaatt aatgctcgcg
    28441 ggtctggtgg aactgaacgt ccggataacg ttcctgtgcc aggcgcaggt tgaccatgct
    28501 ggtagcgatg taagcgaggt tatcgccgcc atcaagcgcc agttggcttt cgttcttacg
    28561 cttgaactct tcgaatttct tcgcgtctgc acattctacc cagcgggcag tggcaacgtt
    28621 gactgactca tacactgctt caacgttgta ttcgctcttc aggcgcgcta ccaccacatc
    28681 aaactgcagc acaccaaccg caccaacgat cagatcgttg ttggagattg gacggaacac
    28741 ctgcaccgcg ccctcttcgg aaagctgtac cagccctttg agcagctgtt tttgcttcag
    28801 cggatctttc aggcggatac gacggaacag ttctggtgcg aagttcggaa taccggtgaa
    28861 cttcatcatc tcaccctggg taaaggtgtc gccgatctga atggtgccgt ggttgtgcag
    28921 gccgaggata tcgcccggat acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt
    28981 cagcgcgtcg gagatcacca catctttcgc agtgcgcacc tggcgcagct tcatgccttt
    29041 ttcatattta ccggacacca cacgcataaa cgccacgcgg tcgcggtgtt tcgggtccat
    29101 gttggcctga attttaaata cgaagccggt aaatttatct tcgctcgctt ctacggtacg
    29161 ggtatcggtc tgacgcggca tcggcgcagg cgcccactcc accaggccat ccagcatatg
    29221 atcaacgccg aagttaccca gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag
    29281 gaacagctct ttgtcgaact cgttagacgc gcctttcacc agttccagtt cgtcacgcag
    29341 ctgctgtgcc agatcttcac caaccgcagc atcgagatcc gggttattca gccctttaac
    29401 aatgcggact tcctgaatgg tgtgcccttt accgctctga tagagatagg tttcatcttt
    29461 ataaaggtgg taaacgcctt taaacagctt gccgcagcca attggccagg tgatcggtgc
    29521 gcagccaatt ttcagctcgt tctcaacttc atcgagcaat tccatcgggt cgcggatatc
    29581 acggtcaagt ttgttcataa aggtgaggat cggcgtgtcg cgcagacggg taacttccat
    29641 cagcttacgg gtacgatctt caacaccttt tgcggcgtcg ataaccatca ggcagcagtc
    29701 caccgccgtc agggtacgat aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag
    29761 caggttaacc aggcaatcgt gatacggaaa ctgcatcaca gacgtagtaa tggagatccc
    29821 acgctgcttt tccatctcca tccagtccga cttagcgtgc tggttggaac cacggccttt
    29881 tactgtaccg gcggtctgaa tggcctgtcc gaacagcagc accttctcgg tgatggtagt
    29941 cttaccggcg tccgggtgag aaataatggc aaaagtgcgg cgcttcgcca cctcttgcaa
    30001 ataaggagac aacgtcataa ttctttcttc ttgagtaagc ggcatcgcgc cgcgcttgtt
    30061 gaaaacgaaa aattgcggct attttaccca tcaacggggg ggaggcaatc agtgtttaca
    30121 caggagctgc tccagttcgt gcaacgaaga aacggtccag gtgggcgcga tgccttctgg
    30181 ttgctcgcga tggtgtgcat tcagccagca ggtcgcaagc ccggcgttga tgccaccgag
    30241 aatatcggac tcggcagtgt cgccaaccat cagcacgcgt gaacggtcag gattgcccgc
    30301 ctgttccagc gcataatcga aaattttctt attcggtttg gcaacgccaa cttcttcgga
    30361 aatcaccagc aaatcgaagt aatcacgcag gcccgtgcgt tccagacgca cctgttgcaa
    30421 ggcactaaag ccgttggtga tgatgccgat tttggcgttg ccacgaatgg cgttaagcag
    30481 agaaaccgcg cccggcagcg gcgtgcagat ttccgccatc gcattaataa aggcttcgtt
    30541 gagtttacct ggctcgacgt tcagccgttc ggcccagctc tcaaaccgcc cgtgctgaag
    30601 ctgtaatgaa gtgatcgcgc cgttttgata atccacccac agtggcttgt taacggcctg
    30661 atagtcctga aaatcttcag cggtaaaggt gacgctgtaa tcaagaaaca tccgctgcag
    30721 gccggtgaat gagtcaaagg taaacagcgt ttcatcggca tcaaagaaaa tccagtccca
    30781 cttcattatt ccaccttgtc ttacatactg attggcaacg ccatgatgat ggcgtcttcg
    30841 cgaccgtccg tggtggggta gtaattgcgg cgaatcgtcg cctcgttaaa gcctaaactt
    30901 tcgtacaggg caatggcggc agcatttgag gcacggactt ccagccatag tgtcgccacg
    30961 ccgcgttttt ccagttcgtc gatcagatgt tccagcagcg cccttcccaa tccctgacgc
    31021 tgatagtcag gatcgaccgc aatattgaac aatgtagctt catccagcac cacttgcgta
    31081 atcgcaaacg ccgccatttt gccgttttgc gttaactgaa agttgagata acgctcgccc
    31141 tggttgctgg caaaggtttt ttcactccac ggaaaggcgt gggcgcgttg ttcaatgtgg
    31201 taagccgctg gtaaatcagt cgtttcgagg gaagaaatcg tgttcatatg tgcaaatttg
    31261 ttgccataac gcggcgcgtg ccgttgggtt tgcccgtaat tcggtgagcg ccggtgatgc
    31321 cacctgagcg ccttccagtg atagcggttc gtcagtaccc aaccgccagc tgttgcagcg
    31381 actgccttgc ggcagcatcg cgattttttc tggcgtcagt tgcagcactt gatcggggct
    31441 gacggttaat gcgcgcagaa catcgctcac taaaggatca gtcagggcgg gaagatcgtt
    31501 tgccaccatc accagacgga cgtgtgccgg gatcgcaatg gcaatctccc cctgcaacgc
    31561 gccagggcga cgcagcgacc actgggtaat gcccagttgc tgtaactgcc agtctcgtcg
    31621 ggatgtcata gcgaatcgct cctgttgtca ggggcgcaaa tatagcaaat tcgtcgatac
    31681 cgcgccaaca tatggctata atcgccgcca gtatcaattg aggagcattc catgtctgca
    31741 tttaccccgg caagtgaagt cttgctgcgt cacagtgatg atttcgaaca aagccgtatt
    31801 ctgtttgccg gagacttaca ggatgacctg cccgcgcgtt tagataccgc ggccagccgt
    31861 gctcataccc agcaattcca gcattggcag gtgttaagcc gccagatggg ggataacgcc
    31921 cgttttagtc tggtcgccac ggcggatgat gtcgcagatt gcgatacgct gatttactac
    31981 tggccgaaga acaaaccgga agcccagttc cagttgatga atttactttc tctgcttccg
    32041 gtggggacgg atatttttgt tgttggcgag aaccgcagcg gcgtgcgcag cgccgagcag
    32101 atgctggctg attatgcgcc gttgaataaa gtcgacagcg cccgtcgctg tggcctctat
    32161 tttggtcgtc tggaaaaaca gccggtattt gatgccgata aattctgggg cgaatacagc
    32221 gtcgatggcc tgacggtcaa aacgctgcct ggcgtgttta gccgcgacgg tctggatgtc
    32281 ggtagccagt tgctgctctc gacgttaacc ccgcacacga aaggtaaagt gctggatgtc
    32341 ggctgtggcg cgggcgtgct ttcggttgcc tttgcgcgcc attcgccgaa aattcgtctc
    32401 accttgtgcg atgtctctgc gccagcggta gaagccagcc gcgcaacact tgcggccaac
    32461 ggtgttgaag gtgaagtctt tgccagcaac gtcttttccg gggtgaaagg tcgttttgat
    32521 atgatcatct ccaacccgcc gttccacgat gggatgcaaa ccagcctgga tgcggcgcaa
    32581 acgctgattc gcggtgcggt gcgtcatctt aatagcggcg gcgagctgcg aattgtagcg
    32641 aacgccttcc tgccttatcc ggacgtgctg gatgagacat ttggcttcca cgaagtgatc
    32701 gcgcaaacag ggcgcttcaa ggtgtatcgc gccattatga cccgccaggc gaagaaaggt
    32761 taattatctc gccgaatacc gtgtcggatg cggcgtgaac gccttatccg acttacaaaa
    32821 cctgcacgtt agtctcctga tggacatttt tccagcaatt acacatcagg cgataattaa
    32881 ctattgacga aaagctgaaa accactagaa tgcgcctccg tggtagcaat tctttttaag
    32941 aattgatggt atgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc
    33001 cttacggacg tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcctttctt
    33061 gtttcttccc tgttttttca tccctgattc aaattcatta tcatcagcat gatgccagca
    33121 ataagcgcca ggcacgatgg tagcaaaatg taatgccgca actgtttgtg atacagcatc
    33181 accggcgtca atactaatcc catcagaatg acaatccgcc actgatccgg cgataaatcc
    33241 gcatacatca gccctgcaat cagaaatccc ggcagcaaca ctccccagcc actgcccagc
    33301 gtacgttgca acatggtttt atctctttca ttgataatga taaccaatat catatgataa
    33361 tttttatcac ttgcaagcca gagaaatccc ttgctatcgg gtaaacctat cgctaggatt
    33421 agcaatcatt atcatttaga ttactatccc gattatggcc tatcgttccg caccgctcta
    33481 tgaagatatc atctggcgaa cgcatctcca gccacaggat gctaggcttg cacaagccgt
    33541 acgtgcgacg atcgccgaac atcgtgaaca tttgctggag tttatccgcc tggatgaacc
    33601 cgctccgctt aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct
    33661 actggcggtc tattccgatc atatctatcg caaccaacca acgatgatcc gcgagtacaa
    33721 accgctgatc tcactatggg cacaatggta tatcggcctg atggtgccac cattaatgct
    33781 ggcgctactg acgcaggaaa aagcattaga tgtgtcgccg gaacatttcc atgctgagtt
    33841 tcacgaaacc ggacgcgtcg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac
    33901 accacattcg ccgcagcagc gaatggaaac gttaatcagc caggcgctgg ttccggttgt
    33961 gcaggcacta gaagcgaccg gagagatcaa cggcaaactt atctggagta ataccggtta
    34021 tttgatcaac tggtatctta ctgagatgaa gcaactgctc ggcgaggcga cggttgaatc
    34081 gctgcgccat gccctctttt ttgagaaaac gcttactacc ggtgaagata atccactctg
    34141 gcgtaccgtg gtgctacgcg acggcctgct ggtgcgccgc acttgctgcc agcgttatcg
    34201 cttaccggat gtacagcaat gtggcgattg tacgctgaaa tgaaagcact gccggggaag
    34261 taaacccggc atcatgcgga ttaataggga tgcaacacat tacttgtttc gcaatgccgc
    34321 atacataaca gaatatctgc ggcctccaac aaccctgcgt cagaactgac gcccagtttc
    34381 gacatcacat taaatttatg tgcacggatc gttttgatat tgcgtttaag ctgctcggca
    34441 atttgtgtca ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt
    34501 aacgtccggc tttggttaat gtaccactga ttgttcagtc ggtcggtcgc ctgacgtaca
    34561 ccatttaatg acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat
    34621 acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat atcatcatcc
    34681 gcaataacta aacgccgggt acgcggaaac ttaatcgcca gttcagtcag gcaagataat
    34741 ccctctctgc gctcacttct catggcagaa aaagaaaaaa tgaccgccgc aaacgagatc
    34801 cgcgacatgg catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggttg
    34861 cccgcaaact ttctgaataa actctcaata ccgatactac tcatgacgca tttttctatg
    34921 agtgcgacat ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag
    34981 aaggcaagtt aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt
    35041 tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg ggcgctgata gttttattac
    35101 ttttcttaag caatgaagct atctggttga tgccccatcc tttacctaat agccgcagta
    35161 ccttgcgctc tgaaagagtg agaatgacgg agcgatagct aaaatcatga gtttcagtca
    35221 ttgccggaga ggtcagcatg gcgctgatac gctctgcgtg cgtattgccg gaacgtacgg
    35281 tcttgaccag attctcaatg ggttcaacat cagacaataa cgtcgccgta gggcacatga
    35341 gcagttctac agcctgggaa taccaagagc gtgacaccat gaaaacccag tgaatttccc
    35401 gatattgtga gattaaagaa taataatgtt cacaaacaga acgtggatct tcactttcac
    35461 cggctaaatc agcaatgact aatccggaac gacgtaattg taataaggta aggtcctccg
    35521 cagaggcgct ggatattatt tcatattcag ggaagtgagt cctcatgacc tcttttaacc
    35581 ctgcttgcat aacaagtatt ttactgataa caattccatt tttgcagcat cctggcaaca
    35641 tatttcctcc acatccactg tctaaaaatt atgatggttt attcaggata ttcattcctt
    35701 aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat tcactttcat
    35761 atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt agcatcaaat
    35821 gata
//
LOCUS       DAZGXS010000032        35408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGXS010000032 DAZGXS010000000
VERSION     DAZGXS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            79..618
                     /gene="elfA"
                     /locus_tag="ACHKG5_001759"
     CDS             79..618
                     /gene="elfA"
                     /locus_tag="ACHKG5_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867878.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            701..1402
                     /gene="elfD"
                     /locus_tag="ACHKG5_001760"
     CDS             701..1402
                     /gene="elfD"
                     /locus_tag="ACHKG5_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0867879.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1427..4026
                     /gene="elfC"
                     /locus_tag="ACHKG5_001761"
                     /pseudo
     CDS             1427..4026
                     /gene="elfC"
                     /locus_tag="ACHKG5_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4017..5087
                     /gene="elfG"
                     /locus_tag="ACHKG5_001762"
     CDS             4017..5087
                     /gene="elfG"
                     /locus_tag="ACHKG5_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867880.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAACSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5115..5642
                     /gene="ycbU"
                     /locus_tag="ACHKG5_001763"
     CDS             5115..5642
                     /gene="ycbU"
                     /locus_tag="ACHKG5_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867881.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5650..6165
                     /gene="ycbV"
                     /locus_tag="ACHKG5_001764"
     CDS             5650..6165
                     /gene="ycbV"
                     /locus_tag="ACHKG5_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0867882.1"
                     /translation="MLKRIIWILFLLGLTGGGELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6131..6867
                     /gene="ycbF"
                     /locus_tag="ACHKG5_001765"
                     /pseudo
     CDS             6131..6867
                     /gene="ycbF"
                     /locus_tag="ACHKG5_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6978..7988
                     /gene="pyrD"
                     /locus_tag="ACHKG5_001766"
     CDS             6978..7988
                     /gene="pyrD"
                     /locus_tag="ACHKG5_001766"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0867883.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8162..8704
                     /gene="zapC"
                     /locus_tag="ACHKG5_001767"
     CDS             8162..8704
                     /gene="zapC"
                     /locus_tag="ACHKG5_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0867884.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8701..9810)
                     /gene="ycbX"
                     /locus_tag="ACHKG5_001768"
     CDS             complement(8701..9810)
                     /gene="ycbX"
                     /locus_tag="ACHKG5_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0867885.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10054..12162
                     /gene="rlmKL"
                     /locus_tag="ACHKG5_001769"
     CDS             10054..12162
                     /gene="rlmKL"
                     /locus_tag="ACHKG5_001769"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0867886.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12174..14081
                     /gene="uup"
                     /locus_tag="ACHKG5_001770"
     CDS             12174..14081
                     /gene="uup"
                     /locus_tag="ACHKG5_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0867887.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14211..15464
                     /gene="pqiA"
                     /locus_tag="ACHKG5_001771"
     CDS             14211..15464
                     /gene="pqiA"
                     /locus_tag="ACHKG5_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0867888.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15469..17109
                     /gene="pqiB"
                     /locus_tag="ACHKG5_001772"
     CDS             15469..17109
                     /gene="pqiB"
                     /locus_tag="ACHKG5_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0867889.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17106..17669
                     /gene="pqiC"
                     /locus_tag="ACHKG5_001773"
     CDS             17106..17669
                     /gene="pqiC"
                     /locus_tag="ACHKG5_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0867890.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17925..18092
                     /gene="rmf"
                     /locus_tag="ACHKG5_001774"
     CDS             17925..18092
                     /gene="rmf"
                     /locus_tag="ACHKG5_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0867891.1"
                     /translation="MKRQKRDRLERAHQRGYQVGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18162..18680)
                     /gene="fabA"
                     /locus_tag="ACHKG5_001775"
     CDS             complement(18162..18680)
                     /gene="fabA"
                     /locus_tag="ACHKG5_001775"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0867892.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18749..20509)
                     /gene="ycbZ"
                     /locus_tag="ACHKG5_001776"
     CDS             complement(18749..20509)
                     /gene="ycbZ"
                     /locus_tag="ACHKG5_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0867893.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20695..21147
                     /gene="matP"
                     /locus_tag="ACHKG5_001777"
     CDS             20695..21147
                     /gene="matP"
                     /locus_tag="ACHKG5_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0867894.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21223..22263)
                     /gene="ompA"
                     /locus_tag="ACHKG5_001778"
     CDS             complement(21223..22263)
                     /gene="ompA"
                     /locus_tag="ACHKG5_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0867895.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22620..23129)
                     /gene="sulA"
                     /locus_tag="ACHKG5_001779"
     CDS             complement(22620..23129)
                     /gene="sulA"
                     /locus_tag="ACHKG5_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0867896.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23348..23977
                     /gene="sxy"
                     /locus_tag="ACHKG5_001780"
     CDS             23348..23977
                     /gene="sxy"
                     /locus_tag="ACHKG5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0867897.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23940..26102)
                     /gene="yccS"
                     /locus_tag="ACHKG5_001781"
     CDS             complement(23940..26102)
                     /gene="yccS"
                     /locus_tag="ACHKG5_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0867898.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26112..26558)
                     /gene="yccF"
                     /locus_tag="ACHKG5_001782"
     CDS             complement(26112..26558)
                     /gene="yccF"
                     /locus_tag="ACHKG5_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0867899.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26681..28735
                     /gene="helD"
                     /locus_tag="ACHKG5_001783"
     CDS             26681..28735
                     /gene="helD"
                     /locus_tag="ACHKG5_001783"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0867900.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28767..29225)
                     /gene="mgsA"
                     /locus_tag="ACHKG5_001784"
     CDS             complement(28767..29225)
                     /gene="mgsA"
                     /locus_tag="ACHKG5_001784"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0867901.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29321..29983)
                     /gene="yccT"
                     /locus_tag="ACHKG5_001785"
     CDS             complement(29321..29983)
                     /gene="yccT"
                     /locus_tag="ACHKG5_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0867902.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30156..30569
                     /gene="yccU"
                     /locus_tag="ACHKG5_001786"
     CDS             30156..30569
                     /gene="yccU"
                     /locus_tag="ACHKG5_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0867903.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30614..30931)
                     /gene="hspQ"
                     /locus_tag="ACHKG5_001787"
     CDS             complement(30614..30931)
                     /gene="hspQ"
                     /locus_tag="ACHKG5_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0867904.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30989..32179)
                     /gene="rlmI"
                     /locus_tag="ACHKG5_001788"
     CDS             complement(30989..32179)
                     /gene="rlmI"
                     /locus_tag="ACHKG5_001788"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0867905.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32274..32552
                     /gene="yccX"
                     /locus_tag="ACHKG5_001789"
     CDS             32274..32552
                     /gene="yccX"
                     /locus_tag="ACHKG5_001789"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0867906.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32549..32878)
                     /gene="tusE"
                     /locus_tag="ACHKG5_001790"
     CDS             complement(32549..32878)
                     /gene="tusE"
                     /locus_tag="ACHKG5_001790"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0867907.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32969..33628)
                     /gene="yccA"
                     /locus_tag="ACHKG5_001791"
     CDS             complement(32969..33628)
                     /gene="yccA"
                     /locus_tag="ACHKG5_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0867908.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34036..35055)
                     /locus_tag="ACHKG5_001792"
     CDS             complement(34036..35055)
                     /locus_tag="ACHKG5_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0867909.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35033..35275)
                     /locus_tag="ACHKG5_001793"
     CDS             complement(35033..35275)
                     /locus_tag="ACHKG5_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0867910.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         9125 a         8415 c         9169 g         8699 t
ORIGIN      
        1 cggacaatta ttgttattat taagtctaat gtcatttaaa ggcattatca tatttacgaa
       61 gagatcagga tgataacgat gaaaaaaagt gtattgacgg cgtttataac tgtggtatgt
      121 gcaacgtcca gcgttatggc tgctgatgat aatgctatca cggatggctc agtaacattt
      181 aatggtaaag ttattgctcc agcttgtacc ctggtagctg cgacgaaaga ttccgtggtg
      241 actttgccag atgttagtgc cacgaagttg caaaccaatg gtcaggtttc tggcgtgcaa
      301 actgatgtgc caattgaatt aaaagattgt gatactaccg taacaaaaaa tgcaacgttc
      361 acctttaatg gcactgcgga tactactcag attacagcgt ttgctaacca ggcctcatct
      421 gatgctgcta caaacgtggc cctgcaaatg tatatgaatg atggtacaac ggccatcaag
      481 ccagacacag aaaccgggaa cattttgttg caagatggag atcagacgtt gacttttaaa
      541 gttgattata tcgctacggg gaaagcgact tctggtaatg tgaatgcggt aacaaatttc
      601 catattaact attattaata gaactcatta attgttttat taattagtac ccctccagtg
      661 ttatggaggg gatattcata ttttttaaga gtgactattt atgaaaactt gcataacaaa
      721 gggaattgtg accgtaagtt taacggcaat attactcagc tgttcgtcca catgggcggc
      781 aggtaaaggc ggggttggac ttgcagcaac acgtcttgtt tattcggaag gtgaagagca
      841 aatttcactg ggtgtacgta acaccagtcc ggatgttcct tatctgattc agtcatgggt
      901 gatgacccca gataataaaa aatcagcgga ctttattatt acaccaccgc tatttgtgct
      961 gaatccggca aacgagaatc tgttacgcat tatgtacatt ggcgcgccgt tggcgaaaga
     1021 cagagaaact cttttcttca ctagcgtacg ggcagtccct tcaacaacga agcgggaaga
     1081 aggcaatacc ctgaagattg ccacacaaag cgtcatcaaa cttttctggc gaccaaaagg
     1141 tttagcgtat cccttaggcg aggctccggc gaaactgcgt tgcacttcgt cagctgacat
     1201 ggttacggtc agtaacccaa caccttattt cattaccctg acagacctga aaataggtgg
     1261 aaagttagtt aaaaatcaaa tgatttcccc ctttgataaa taccaattct ctctgccaaa
     1321 gggtgccaaa aatagcagcg taacgtatcg aaccatcaat gactacgggg cggaaacgcc
     1381 gcaactcaac tgtaaatcgt aattcgtctt cagttaagag agcgagatgt atagaactca
     1441 ccgacaacac agcctgttaa gctctggtgg agtgccatcg tttattggtg ggctggtggt
     1501 gtttgtgtcg gcagcgttca atgcacaagc tgaaacctgg ttcgatccag cctttttcaa
     1561 agatgatccc tcaatggtgg ccgatttgtc tcgtttcgaa aaaggacaaa aaataacgcc
     1621 aggggtttat cgtgtcgata ttgttctgaa tcagacaatt gtagatacgc gcaacgtcaa
     1681 ttttgttgag ataacgccag agaaggggat tgccgcctgt ttgacgactg aaagcctgga
     1741 tgcaatgggc gtgaatactg atgcatttcc ggcttttaaa caactggaca aacaagcgtg
     1801 tgcgccattg gcggagatta ttccggatgc cagcgtaact tttaatgtga ataaactccg
     1861 tctggaaatt tcagtaccgc aaattgccat caaaagtaat gctcgtggtt atgtccccct
     1921 gaacgttggg atgaagggat caacgcgcta ttactgggat attcatttag cggggctaac
     1981 agtattcata gcagcgcaga cagtgattct ggcgacagtt attttctgaa tttaaacagt
     2041 ggcgttaatt taggcccatg gagattgcgc aacaattcaa catggagtcg tagtagtggc
     2101 caaaccgcag aatggaagaa tctcagcagc tatttgcaac gggcggttat tcctctgaaa
     2161 ggcgaactga ccgtaggtga tgattatact gcaggcgatt ttttcgatag tgtcagcttt
     2221 cgtggtgtgc agctggcgtc agatgacaac atgctgccag acagcctgaa agggtttgcg
     2281 cctgtggtgc gtggtatcgc caaaagcaat gcccagataa cgattaagca aaatggttac
     2341 accatttacc aaacttatgt atcgcctggt gcttttgaaa ttagtgatat ctattccacg
     2401 tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg acggcagcgt caatagctac
     2461 agcgtaccct tttccagcgt gccattactc cagcgtcagg ggcgaatcaa atacgcggtg
     2521 acactggcga aatacagaac caatagtaat gaacagcagg agagcaaatt tgcccaggcc
     2581 acgttgcagt ggggcggacc gtggggaacg acctggtatg gtggtggaca atatgctgaa
     2641 tattaccgtg ctgccatgtt tggtctggga tttaacctcg gcgatttcgg agcaatttcg
     2701 ttcgatgtga cccaggcgaa gagtacgctg gcagaccaaa gcgaacataa aggtcagtca
     2761 tatcgttttc tgtatgccaa aacgctcaac caattgggta ctaactttca attgatgggc
     2821 tatcgctatt cgacgtcggg tttctacacc ctttccgaca ccatgtataa acatatggat
     2881 ggctacgaat ttaatgacgg tgatgatgaa gatacgccga tgtggtcgcg ttattacaat
     2941 ttgttttaca ccaaacgtgg caaactgcag gtcaatatct cccagcaatt aagcgagtac
     3001 ggttcgtttt atttaagtgg tagccagcaa acttactggc ataccgatca acaggatcgg
     3061 ctattacagt ttggctataa cacgcaaatt aaagatctct cgctggggat ctcctggaac
     3121 tacagtaagt cccgtggcca acctgatgct gatcaggtgt ttgcacttaa tttttccctg
     3181 ccactcaatc tgttgctctc cagaagtaat gatagctata ccagcaaaaa aaattacgcc
     3241 tggatgacct ctaacaccag tatcgataac gaagggcaca ctacacaaaa cctgggttta
     3301 acggagacac tactcgatga cggtaacctg agctacagcg tgcaacaggg atataacagc
     3361 gaggggaaaa cggctaatgg cagcgccagc atggactaca aaggggcgtt tgcagatgcc
     3421 cgagtgggct acaactacag cgataacggc agtcaacaac aactgaacta cgctctttca
     3481 ggcagtttag ttgcccattc acagggcatt accctggggc aatcactggg ggaaactaac
     3541 gttctgattg cagcaccagg cgcagagaat actcgtgtgg cgaacagcac cgggctgaaa
     3601 actgactggc gcggatatac cgttgttcct tatgccactt cttatcgtga aaatcgaatc
     3661 gcacttgatg cggcgtcgtt aaaacgtaac gtggatcttg aaaatgcagt agtcaacgtg
     3721 gttcccacca aaggggcgtt ggttctggcg gagttcaatg cccatgcggg agcaagggta
     3781 ttaatgaaaa catcaaagca gggtatatcg ctgcgttttg gcgcgatagc gacgctggac
     3841 ggcgtacaga ctaatagcgg cataattgat gatgatggct cgctctatat ggcgggttta
     3901 ccggcgaagg gaacaataac cgtgcgctgg ggcgaagctc ccgatcaaat ttgtcatatc
     3961 agttacgagc ttaccgaaca acaaattaac tctgcgatta cgcgaatgga tgccatatgc
     4021 agataatctt tggagaaaaa tgcgtgtcat tactacgact attttttgcc gccgtcttaa
     4081 tgctatggtg cgctcaaacc gctgcttgta gcgggcagtg tcataccact caggggaatc
     4141 cgtatattgg cgtcaatttt ggcgttaaaa ccctggagga agaagaaaat acgactgggg
     4201 tagtaaaaga caaattttat cagtggaacg aatcgaatga ttattatgtt tcctgtgatt
     4261 gcgataaaga caatgtcaga agtggccgat gggcattcgc cgcggattca ccgttagtct
     4321 atttaggcga caactggtac aaaattaatg actatcttgc cgccaaagtt ttattgcagg
     4381 ttaaaggcag ttctcctaca gcggttcctt tcgaaaacgt ggggactggg gcagataccc
     4441 ggtggcatat ttgtgacccc ggcggtcaac gtttaggcgg ccagggagct agcggtaata
     4501 gcggtagctt ttccctgaaa atattgcagc cgttcgttgg ttcggtcgtc attcctccta
     4561 tggcgctggc gcgattattt gaatgctaca acatacccgc aggtgattcc tgcacgacta
     4621 caggcacacc ggttttagtg tattacctgt ctggtactat caattcactt ggctcatgtt
     4681 ccgtcaatgc cggagaaaca atcgaggtcg atctgggcga cgtatttgcg gctaactttc
     4741 gtgttgtagg gcataagcct cttggggcca gaacggcaga acttgcaatt ccagtcaggt
     4801 gtaacacggg aaacgcgggg ttagttaacg tcaacctgag tctgacggca accacagacc
     4861 ccagctatcc ccaggcgatt aagacgtcac gtcctggcgt gggcgtggtg gtgaccgata
     4921 gccagaacaa cattatttcc cctgctggtg gaacattacc gctctctatt cctgatgatg
     4981 cagacagtat cgcgcgaatg aatgtctatc cagtcagcac gacaggtgta ccaccagaaa
     5041 ccgggcgatt tgaagccacg gcaacggtga gaataaattt tgattaacgc gtgaacgtat
     5101 gaagaaaaaa aacgatattt cagtgcgtta tcttgttctt tagcattctt aacatccatg
     5161 tcgggatggc tgggcctgaa caagttagta tgcatattta tgggaatgtg gtcgatcagg
     5221 gctgtgatgt cgccaccaaa agtgcattac aaaatattca tattggtgat tttaatatca
     5281 gtgattttca ggccgcgaat accgtaagca ctgctgctga tttgaatatc gatatcaccg
     5341 gttgtgccgc tggtattact ggcgcggacg tcctttttag cggcgaggct gacacccttg
     5401 cgccgacact gctcaaacta actgacacag gcggaagcgg tggtatggca acggggattg
     5461 ccgtgcaaat tcttgatgcg caaagtcagc aagaaatccc gctcaatcag gtccagcctc
     5521 ttacgccctt aaaagccggg gataacacac tcaaatatca acttcgttat aagtccacaa
     5581 aggcgggagc aacgggcggt aatgcgacgg cggttctcta ttttgatctg gtttaccagt
     5641 gaaggagtga tgttgaaacg gataatctgg attctgttct tactgggatt aacggggggg
     5701 ggtgagctat ttgcccatga tggcacggtc aacattagcg gatcgtttcg ccgtaataca
     5761 tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt taggcgatgt ctcactgaca
     5821 cgtttttctc atgggaatta tggcccggag aaatctttca tcattaattt gcaggattgc
     5881 ggaacagatg tcagcacggt tgacgtcacc ttttcaggaa cccccgacgg agtgcagagc
     5941 gaaatgttgt ccattgaaag tggtacggat gcagccagcg ggctggcaat tgcgattctg
     6001 gatgatgcga agatattgat tccgcttaac caggcgagta aagactacag cctacatagc
     6061 ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg tcaatagcga tgtgcagtcg
     6121 ggtaaagtga atgccagcgc gacatttgta cttcattatg actaatacgt ggaacagatt
     6181 ggcgctcttg attttcgccg ttttatcgtt gctggtggcg ggtgaattgc aggcaggagt
     6241 ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga gaatcgatat ctattttact
     6301 gactaatacc agtcaggaat cctggcttat taacagtaaa atcaaccgcc caacgcgttg
     6361 ggccgggggt gaagcgtcga cagtgccagc accattactg gccgctccgc cacttattct
     6421 cctgaagccc ggtacgacag gcacgttgcg cttgctgaga acggaaagcg acatcttgcc
     6481 tgtggatcgc gaaacgctat ttgagttaag cattgccagc gtgccatccg gcaaagttga
     6541 aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa ctgttctggc gacccgaagg
     6601 ttgccgggcg acccgctgga agcttatcaa caattacgct ggacacggaa gtcgcagggt
     6661 gtacaactca ctaacccaac gccttattac attaacctga ttcaggtgag cgtaaatggt
     6721 aaagcgttaa gtaatgtggg agttgtgccg cctaaaagcc agcgtcagac aagctggtgt
     6781 caggcaatag caccgtgtca tgttgcctgg cgggcgataa atgattacgg cgggttgagt
     6841 gcaaagaagg agcaaaatct gccctgaaac aggttcggaa aacgtttgcg ttttttttgc
     6901 cgcaggtcaa ttcccttttg gtccgaactc gcacataata cgcccccggt ttgcacaccg
     6961 ggaatccagg agagttcatg tactacccct tcgttcgtaa agcccttttc cagctcgatc
     7021 cagagcgcgc tcatgagttt acttttcagc aattacgccg cattaccgga acaccgtttg
     7081 aagcactggt gcggcagaaa gtgcctgcaa aacctgttaa ctgcatgggc ctgacgttta
     7141 aaaatccgct tggtctggca gccggtcttg acaaagacgg ggagtgcatt gatgcgttag
     7201 gcgcgatggg atttggatcg atagagatcg gtaccgtcac gccacgtcca cagccaggta
     7261 atgacaagcc gcgtctcttt cgtctggtag atgccgaagg tttgatcaac cgtatgggct
     7321 ttaataatct tggcgttgat aacctcgtag agaacgtaaa aaaagcccat tatgacggcg
     7381 tcctgggtat taacatcggc aaaaataaag atacgccagt agagcagggc aaagatgact
     7441 atctgatttg tatggaaaaa atctatgcct atgcgggata tatcgccatc aatatttcat
     7501 cgccaaatac cccaggatta cgcacactgc aatatggtga agcgctggat gatctcttaa
     7561 ctgcgattaa aaataaacaa aatgatttgc aagcgatgca ccataaatat gtgccgatcg
     7621 cagtgaagat cgcgccggat ctttctgaag aagaattgat ccaggttgcc gatagtttag
     7681 ttcgccataa tattgatggc gttattgcaa ccaataccac actcgatcgt tctctggttc
     7741 agggaatgaa aaattgcgat caaaccggtg gcttaagtgg tcgtccgctt cagttaaaaa
     7801 gcaccgaaat tattcgccgc ttgtcactgg aattaaacgg tcgcttaccg atcatcggtg
     7861 ttggcggcat cgactcggtt atcgctgcgc gtgaaaagat tgctgcgggt gcctcactgg
     7921 tgcaaattta ttctggtttt atttttaaag gtccgccgct gattaaagaa atcgttaccc
     7981 atatctaatt atttcttcga attcctcttt ataagataat cagggcttta ttttcagccc
     8041 tggttgtttt atattcattg ctgttgctta tttagacatt ttgtactttt attgttgagg
     8101 ttattaagcg aagcgacaat ggattgtgtt gctgcggttt tatagggtag gggagaggca
     8161 gatgcgaatt aaaccagacg ataactggcg ttggtattac gatgaagagc acgatcgtat
     8221 gatgctcgat ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca aaatgttgac
     8281 tccagatgcc ttttctcccg caggcttttg cgttgacgac gccgcgctct atttctcttt
     8341 tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa aaagccgaac tggtgctgaa
     8401 tgcactggta gcaattcgct atctcaaacc gcaaatgccg aaaagctggc attttgtttc
     8461 ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc tgcgtttggt tgagcgacac
     8521 taacgagcaa gtgaatttac tggtcgttga atctggcgag aatgccgcgc tatgcctgct
     8581 ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat
     8641 catgaacgac aggctgaaac cgcaggttaa tgttgacagc ttcagcctcg aacaggcagt
     8701 ctaacgcgcc aacttaagtg cagtcttcgg tacacagcta cagcaaagaa tggtgccatc
     8761 atcgcccatt gctgattttt tcagcggcgt gacttcgcct tctaaaagct gaacacggca
     8821 acttccacaa atgcccgcgc ggcaagaata agggatacga attccctgat tttctaattg
     8881 ctccagcaac acctgttggt tatttccacg aaatgcctgt ccctgccagt caatatctac
     8941 attggcgtcc ggttgttgcg tgatgttggc agtatcatca gcggcagctg cgccgtaaat
     9001 ttttgccgga gccgttgtca gaatttccac ctcatcgcca acgcgaatca cgccgctatt
     9061 acgggcaatt aaattctgac caaaatcgac atcgccgtta tcctgggcag tgcggaaaga
     9121 ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc cctttttctg ggctgacggt
     9181 ggtgaaaata cagcggctac aaggtttaac cacatcaaac accacatcac caatgcgaat
     9241 cactttccaa ctatcttctt cccaggctga cgcgccggaa accaccagat tggggcggaa
     9301 ttgctccatt tttacactgg ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt
     9361 agcaagaagg taaggatagc catcagcaaa tgacaggggt acagtgttgt ggcgtttcac
     9421 gcgccgggtc atttgtggcc ccacccagcg taattgcact tcgcgggaga aaaatccact
     9481 taaccatttg ttgatcgctt ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc
     9541 ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac tgctatctgg
     9601 tgcggtgaga tgcaagccat catgcacggg tgaaggagta aatcgtacca tctggggaaa
     9661 ctggcgagcg gtgataaacg taccgtcagg ttccgtgatc ataaagatgc gatcgaaggc
     9721 cagaccactg acatctgcca gagcatgtgt aagaccaatg ccgcgcatcg atttaacagg
     9781 atgaataaaa agccgggtta atgtcgccac ggtgcggtcc tcaaatgaaa ataagccctc
     9841 aactttatga catgacgcgc ttattagcta taatgcgcaa caattttctt agcgcctgtc
     9901 ccattaggct attttatttg tcagtttggc cctgggttgt gctcgaaatc ctcacgtact
     9961 tgtgtacgct ccggtttctc cgcgctgtcc atgtccagac tgactgcaac aattacgcct
    10021 actgcggtag gctcttagag taaaagtgac gatatgaatt ctctgtttgc cagtacggcc
    10081 cgtgggctgg aagagctgtt aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag
    10141 gtggttcagg gtggggtcca tttcaagggc gacacacggc ttgtttacca gagcctgatg
    10201 tggagccgcc tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt ttacagcgat
    10261 ttagacctct atctcggtgt tcaggcgatc aactggacag agatgtttaa tcctggcgcg
    10321 accttcgctg tccacttcag tggtttgaat gacactatcc gcaacagtca gtacggcgcg
    10381 atgaaagtga aagacgcgat cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat
    10441 gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc tgcataaaga aaccgccagt
    10501 atcgcccttg atctcagtgg tgatggttta catctgcgtg gctatcgcga tcgtgctggt
    10561 attgcgccga tcaaagaaac cctggcagcc gctattgtga tgcgatccgg ctggcagcca
    10621 ggaacaccgt tgctcgatcc gatgtgtggt tccggtacgt tgctgattga agcggcgatg
    10681 ctggcgaccg atcgcgcacc aggcttgcac cgtgggcgtt ggggctttag cggctgggcg
    10741 cagcatgatg aagctatctg gcaggaagtg aaagcggaag cgcaaactcg cgcccgtaaa
    10801 ggcctggctg agtatagctc tcatttttac ggttcggaca gcgacgctcg ggtgattcaa
    10861 cgtgcacgca ctaacgcccg tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa
    10921 gatgtcgcgc aactgaccaa tccgctgccg aaagagccgt acggcacagt gttaagcaac
    10981 ccgccatacg gggaacgtct ggacagcgaa ccggcgctga ttgcgctgca tagcctgctg
    11041 ggtcggatca tgaaaaacca gtttggtggc tggaatctct ctttgtttag tgcctcgccg
    11101 gatctgctaa gctgcttgca gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg
    11161 ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca ctccagacag caaaccggcg
    11221 atggtagcgg aagactacgc caaccgcctg cgtaagaacc tcaaaaaatt cgagaagtgg
    11281 gctcgtcagg aagggattga atgttaccgc ctgtatgacg ccgatctgcc ggaatataac
    11341 gttgccgttg accgttatgc cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact
    11401 attgatgcgc acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac catttcggta
    11461 ctggggattg cgccaaacaa actggtgctg aaaacccgtg aacgtcagaa gggcaaaaat
    11521 cagtaccaga aactgggcga gaagggcgag tttctggaag ttaccgaata taacgctcac
    11581 ttgtgggtga acctgacgga ttatctcgat actggtctgt tcctcgatca ccgtatcgcc
    11641 cgtcgtatgc tcggccagat gagcaaaggc aaagatttcc tcaacctgtt ctcttatacc
    11701 ggcagcgcca ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac cgtggatatg
    11761 tcgcgtactt atctggagtg ggcagaacgc aacctgcgtc tgaatggcct aaccgggcgt
    11821 gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttc
    11881 gatctgatct ttatcgatcc gccaaccttc tctaactcaa aacgaatgga agatgcgttt
    11941 gatgttcagc gcgatcatct ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt
    12001 gggacgatca tgttctcgaa caacaaacgt ggcttccgta tggatctcga cggcctggca
    12061 aaactgggac tgaaagcaca agaaattacg caaaaaacgc tctcccagga tttcgcccgt
    12121 aaccgccaga tccacaactg ctggctgatt accgcagcct gaaaggaata gtaatgtcat
    12181 taatcagtat gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc gataacgcag
    12241 aactgcatat cgaagataac gaacgtgttt gtctggtggg ccgcaacggc gcaggcaaat
    12301 cgacgttaat gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc attatttacg
    12361 agcaggattt gattgtagcg cgtctgcaac aggatccgcc gcgtaacgtt gagggtagtg
    12421 tttatgattt cgttgccgaa ggcattgaag aacaagcgga atacctgaaa cgctatcacg
    12481 atatttcgcg cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg
    12541 ttcaggaaca gctggatcac cacaacctgt ggcagctgga aaaccgcatc aacgaagtgc
    12601 tggcgcaact ggggttagat cctaacgttg cgctgtcgtc gctttccggc ggctggttgc
    12661 gtaaagcggc attaggacgc gcgctggtga gcaatccgcg cgtattgctg ctggacgaac
    12721 cgactaacca cctggatatc gaaaccatcg actggctgga agggttcctc aaaaccttca
    12781 atgggacgat tattttcatc tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca
    12841 ttgttgatct cgatcgcggc aagctggtga cctatccggg caattacgat cagtacctgc
    12901 tggaaaaaga agaagccctg cgcgtggaag aactacaaaa tgccgagttt gatcgcaaac
    12961 tggcgcagga agaagtgtgg attcgccagg ggatcaaagc acgccgtacc cgtaatgaag
    13021 gccgcgtacg cgccctgaaa gcgatgcgtc gcgagcgtgg cgaacgtcgc gaagtgatgg
    13081 gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg taagatcgtt ttcgaaatgg
    13141 aagacgtttg ctaccaggtt gacggaaagc aactggtgaa agatttttct gcccaggttc
    13201 tacgtggcga caaaattgcc ctgattgggc cgaatggctg cggcaaaacc acgctgctga
    13261 aactgatgct cggtcagctt caagcggaca gcgggcgtat tcacgttggc accaaactgg
    13321 aagtggctta tttcgatcag caccgcgcgg aactggatcc cgataaaacg gtgatggata
    13381 accttgccga aggtaagcaa gaggtgatgg ttaacggcaa gccacgccac gttttgggct
    13441 atttgcagga cttcctgttc catccgaaac gggcgatgac gccagtacgt gcgctttctg
    13501 gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc aacttattga
    13561 ttcttgacga accgaccaac gatcttgatg tcgaaacgct ggaactgctg gaagaactga
    13621 tcgacagcta ccagggcacg gtattgctgg taagccacga tcgtcagttt gtcgataaca
    13681 ccgttacaga atgctggatc ttcgaaggcg gcggtaaaat tggtcgttat gtcggcggtt
    13741 atcatgatgc ccgtggtcag caagagcagt atgtggcgct caaacagcct gcggtgaaaa
    13801 aaaacgaaga acccgccgcg ccaaaagcag aaactgtaaa acgcagcagt agcaaactaa
    13861 gctataaatt gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat ctggaggcga
    13921 agctggaagc cctacagacg caggtggcgg atgcttcctt cttcagtcag ccgcatgagc
    13981 agacacaaaa agtgctcgcc gatatggctg ctgcagagca ggagctggag caagcctttg
    14041 aacgctggga gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat agtcgattta
    14101 aacagagaca gctgacaacg gtaaaattgc ctgatgaact gggtttatca ggcatgaaaa
    14161 caaacacttt gttagtcatc tgtaaaatag cgcatcatta aggagtacca atgtgcgaac
    14221 atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga catgctggtg gcgttaccgc
    14281 gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt
    14341 gggatgcccc ccggcagcgt ccgaccgcct atgcgttggc tgcactgttc atgctgttgc
    14401 tgtccaactt gtttcctttt gtgaatatga acgttgcagg agttaccagt gaaattacat
    14461 tactggaaat tcccggcgtg cttttttctg aggactacgc cagcctcggc acctttttcc
    14521 tgttgtttgt gcaactggtt cccgcgtttt gtctgataac cattctgtta ctggtgaatc
    14581 gcgcggaatt accggtccgt ttaaaagagc aactggcacg ggtgcttttt caactcaaaa
    14641 cctggggaat ggcggagatt ttcctcgcgg gtgtgctggt cagtttcgtt aaactgatgg
    14701 cttacggcag cattggcgta ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc
    14761 aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg ggacgatatc gccccgatgc
    14821 cagaactgcg ccagccgcta aaaccaggcg tcacggggat acgtcagggg ctgcgttcgt
    14881 gctcctgttg tacggcaatc cttcctgctg atgaacccgt gtgcccgcgc tgtggtacta
    14941 aagggtacgt tcgacgtaga aacagcctgc aatggacact cgcgctgctt gttacgtcca
    15001 tcatgctgta tcttccggct aatattttgc ccatcatggt gacggattta ttaggctcga
    15061 agatgccatc gacgattctc gctggggtca tcctgttatg gagcgaaggc tcttatcccg
    15121 tcgctgcggt gatctttctg gccagtatta tggtgccaac gttaaagatg atcgccatcg
    15181 cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga atgcatttga
    15241 tttatgaagt tgttgagttt gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg
    15301 tgctctcggc gctggtgcgt atgggaggtt taatgagtat ttatccggca atgggtgcat
    15361 taatgtttgc tttagtcgtc ataatgacaa tgttttctgc tatgacgttt gacccgcgtt
    15421 tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc ctgacagtat ggaatctaat
    15481 aatggggaag ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat atttcctatc
    15541 gtcacggcgc tcattggggc ctgggttctt ttttatcatt acagccatca gggaccggaa
    15601 gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac cattaaaagc
    15661 cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg ctgatgattt gacacacgtt
    15721 gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga taccgtcttt
    15781 tgggtggtga aaccgcagat tggtcgagaa gggattagcg gtctgggaac cctgctgtct
    15841 ggggtttata tcgaactgca gccaggcgcg aaaggcagca aaatggataa atacgatttg
    15901 ctggactcgc caccgttggc cccgcctgat gcgaaaggta tccgtgtggt tctcgatagc
    15961 aaaaaagccg ggcagctctc gccaggagat ccggtgctgt tccgtggcta tcgggtaggt
    16021 tcggttgaaa ccagcacctt cgatacgcaa aaacgcaata tcagttatca actgttcatc
    16081 aatgcacctt atgaccgact ggtgaccagc aatgttcgct tctggaaaga tagtggcatt
    16141 gcggttgatc tgacgtcagc gggaatgcgt gtggagatgg gctcattgac aacgctgctg
    16201 agtggcggtg tcagctttga tgtgccggaa ggtctggatt tagggcagcc agtggcaccg
    16261 aaaacagctt tcgttttgta tgatgatcag aagagcattc aggattcgtt gtacaccgat
    16321 cacattgatt atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc gggagctccg
    16381 gtagaattcc ggggtattcg cctcggtacc gtaagcaaag tgccattctt tgcgccgaat
    16441 atgcgtcaga catttaacga tgattaccgt attccggtgc tgattcgtat cgagccagag
    16501 cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg
    16561 aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg tcactggcgc actgtatgtt
    16621 gatctcgatt tctatccaaa tacgcctgca ataaccggta ttcgtgaatt taatggttat
    16681 cagattatcc cgaccgttag cggcggcctg gcgcaaatcc agcaacgact gatggaagcg
    16741 ttggataaga tcaacaaact gccattgaat ccgatgattg aacaggcaac cagtacgctt
    16801 tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc tggatagcat gaacaagatc
    16861 ctcgccagcc agtcgatgca gcagttgccg acggatatgc agtcaacgtt gcgtgaattg
    16921 aatcgcagca tgcagggctt ccagcccggc tccgcagcct acaacaagat ggtggcggat
    16981 atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac gctcaatgag
    17041 aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag atccagagcc gaagagggcg
    17101 aaacaatgaa aaagtggcta gtgacgattg cagcactgtg gctggccgga tgcagctccg
    17161 gcgaaattaa taaaaactat taccagttac ctgtggtgca gagcggtaca caaagtaccg
    17221 ccagccaggg caatcgcctg ttatgggtag agcaggtcgc tgtacctgac tatctggcgg
    17281 ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt gattgccaac aacaacttgt
    17341 gggccagccc gttggatcaa cagttgcgca acaccctggt agccaacctg agcacgcaac
    17401 tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta
    17461 ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt
    17521 tgaaccacca gggacaactg atcaaacgtc cgttccgtct ggaaggagtg caaactcagg
    17581 atggttacga tgagatggtt aaagtgctgg ccggtgtctg gagtcaggaa gccgcttcta
    17641 ttgcacaaga gataaagcgt ctaccttaat tataaagatt tgtaaatata accgtctccg
    17701 gtatgttgcc tgaggcggtt tttttgtttc taacgtgcgg aaaaatttgt tcctcttcac
    17761 attttttgta caaccgacat gcccgtgtag ctcacaaata tgacagtggc gtgaattttg
    17821 cgcattgacg gcagttatga ttcgcggtat tgcttaactg tgattgcaca tttagtaatc
    17881 actgttttct tttccaccag aaaccagtat gagggaaacg aggcatgaag agacaaaaac
    17941 gagatcgcct ggaacgggca catcaacgtg gttatcaggt cggcatcgcc ggacgctcaa
    18001 aagaaatgtg tccctatcag acgctgaatc aaaggtcaca atggctggga ggctggcgag
    18061 aagccatggc ggacagggta gtaatggcct gattctgtct ctttaaaaag aaacctccgc
    18121 attgcggagg tttcgccttt tgatactctg tctgattata atcagaaggc agacgtatcc
    18181 tggaacagac cgactttcag gtcgctggcg gtatagatca gacgaccatc aaccagcact
    18241 tcgccatccg ccaggcccat aatcagacga cggttaacaa tgcgtttaaa gtgaatacgg
    18301 taggtcactt ttttcgctgt cggcagtacc tgaccagtga atttcacttc gccaacgccc
    18361 agcgcgcggc ctttaccttc gccgcccagc cagccgaggt agaaccctac cagctgccac
    18421 attgcgtcca ggcccaggca tcccggcata accggatcgc caataaagtg gcatccgaag
    18481 aaccacagat ccggattgat atccagttct gcttcaacat accctttgtc gaagttacca
    18541 cccgtttcgg tcattttgac cacacggtcc atcatcagca tgttcggtgc tggcaattgc
    18601 gggcctttag cgccaaacag ttcaccgcga ccagaggcaa gaaggtcttc ttttgtatag
    18661 gattcgcgtt tatctaccat gttctctgta agccttattt tattgaagca cgcaggatag
    18721 ctaacacgtg tacgctgaac aagtccgatc agttcggaat aaaccagttc agccaacgta
    18781 atggccatgg aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata cgttcctgga
    18841 tggtttgcat cagcgtcgtt tggccttcgc catcccacac cagatttaat aataacggca
    18901 gtgcgtcagt cacatcgtct actgcccaga tggtgaattt gccttcttct accgctttca
    18961 ccagttcact gtgaagactt aaatggcgaa cgttagctgt ggggataatg acaccttgtt
    19021 tcccggttaa ctcacgttgc tggcaaatag caaagaagcc ttcgattttc tcatttaaac
    19081 caccgaccgg ctgggcgcga ccgaactgat cgactgaacc tgtgatagcg atactctgat
    19141 tcaccggcac atcggcgagg gcgcttatca gggcgcagag ttcagccatc gaggcactat
    19201 ctccatcaac ttcactgtat gactgctcaa atgtcagcga tgctgagaag gggatctgtt
    19261 gctcaagctg tagttccgac atcaggaacg cttgcatgat catcatccct ttcgcatgga
    19321 tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca
    19381 caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga aattcaatga
    19441 ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct
    19501 gaaggatctc atcctgcata cgttcagcga ggaaaccttc gcgccattca cgctgctgca
    19561 gcattaagtt tagctgctcg ccggagaagg tgtcgccatc acacagggag gcgacctctt
    19621 tacactggcg gaggatccac tgcgggctaa gcggaagtgt ttcttgttca ccggtgtagc
    19681 gtgctgcttc gcggataagt accggccagg catccgctcc cggggcaggt aagtgattat
    19741 gtctggcggt aaatgtcacc cagcgacacc actgggttac tgactccgca tcgacaatct
    19801 gcagagtatc ttcaaattcg ctataaatag cctgctctga aagctctggc tccatctcct
    19861 ggaaatcagc caatgattcg cgttcgccta ccagaatgac tttcagcttc aatggcatcg
    19921 aaggcacaga gacggggaga gggcgcgact catcaaacgc aacccagtca aaacgctcgc
    19981 ggttaacgat atttttcagc cgcatccaca gcagaggttg cgccagcagt gtacgcaaag
    20041 agatgatgag aataccgcca tttgcctgat gcaccagacc aggctgcagg gtaatgtcgc
    20101 cattaaactg gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg gcagcgacaa
    20161 cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag cggatggagt
    20221 ggccggaaac ctcatagtga ccgcccacca gttggcctgc atcgctttgt agcgtacgcg
    20281 cggcattggc aataagattc agatactcag actcttccgg ggccttcgcc agcataaaag
    20341 aggaggatgc tcgcgtatgc agcaactgct ccagcgcaaa ttgcagccgc ggttgagtat
    20401 cactgaataa aggatcgttt tcgtcaatca aatgtggctg agcaaatatt tcctgatagc
    20461 tatcggtatc aggaaccagg tcacgccatg caagtttcgt aatggtcaaa gttgatgttt
    20521 tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc actacagcac acgtagaaag
    20581 caccgacaat actcctggca tgggcgttaa agctcacagg atggagattc ttttcttcac
    20641 tggcctaaaa agctgatatt ctgtaaagag ttacacggta acattgagat cgctatgaaa
    20701 tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga agtatctggt gaagaagcat
    20761 cgcgaagggg agttaatcac ccgttacata gaagccagtg ccgcccagga agccgtcgat
    20821 gtgttgctct cgctggaaaa tgaacccgtg ctggtaaatg gctggattga caagcatatg
    20881 aatccggaac tggtcaatcg catgaagcaa accattcggg caagacgaaa gcgccatttt
    20941 aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc tggaatttat tgtctggcaa
    21001 cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt ctgaaacgat tgttcagctg
    21061 attgaagatg cggaaaacaa agagaaatac gcgaataaaa tgtcttctct gaagcaggat
    21121 ctgcaggcat tgctgggtaa ggaataactg acgaaagtca gttcaattta ctaaaggcaa
    21181 aaaaaacccc gcagcagcgg ggtttttcta ccagacgaga acttaagctt gcggctgagt
    21241 tacaacgtct ttgatacctt taacttcgat ctctacgcga cgatccggag ccaggcagtc
    21301 gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg ccagtaaccg ggttggattc
    21361 gcccatacca cgtgcggaga tcttgtctgc cgggatacct ttggagatca ggtaatcaac
    21421 aacagactga gcacggcgct cggacagacc ctggttgtaa gcgtcagaac cgatgcggtc
    21481 ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc aggttgctca gctggctgta
    21541 cagctgatcc agagcagcct gaccttccgg tttcagggtt gctttgttga agttgaacag
    21601 aacgtcagac ttcagagtga agtgcttggt ctgtacttcc ggtgccggag ctggagccgg
    21661 agcaactact ggagctgctt cgccctgacc gaaacggtag gaaacaccca ggctcagcat
    21721 gccgttgtcc ggacgagtgc cgatggtgtg tgcgtcaccg atgttgttgg tccactggta
    21781 ttccagacgg gtagcgattt caggagtgat cgcgtactca acaccgccag cgaagaccgg
    21841 agaaacgccg gtgtcgtggt ttttaccata aacgttggat ttagtgtctg cacgccatac
    21901 cataccaccc agacgagtgt agatgtccag gtcgtcagtg attgggtaac ccagtttagc
    21961 ggtcagttga acgccctgag ctttgtatgc accgttttca acgctgcctt tgtacggcat
    22021 acgacctaac cagtcgtaac ccatttcaaa gccaacatac gggttaacct ggtaaccacc
    22081 aaaagcacca gcgcccagtt ggttttcatg ggtcgggcca ttgttgttga tgaaaccagt
    22141 gtcatggtac tgggaccagc ccagtttagc accagtgtac caggtgttat ctttcggagc
    22201 ggcctgcgct acggtagcga aaccagccag tgccactgca atcgcgatag ctgtcttttt
    22261 cattttttgc gcctcgttat catccaaaat acgccatgaa tatctccaac gagataacac
    22321 ggttaaatcc ttcaccgggg gatctgctca atattaactc taccgatatc ttcggcttat
    22381 gccgagcacc cctggcgatg taaagtctac aacgtagttg aaaacttaca agtgtgaact
    22441 ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt ttgtcagcga
    22501 caatttacag aagagaatcg cggaaaccgc ttcagacaag cctccgcaag gaaaattagt
    22561 cacgactgaa agcattggct gggcgacaaa aaaagttcca ggattaatcc taaatttact
    22621 taatgataca aattagagtg aatttttagc ccggaaagtt gtctcgtggc gtgagaggat
    22681 gcgcttaccg gacgcataat aaaccccata gcgttacctt catttgccgc atcaacaagt
    22741 tcagcatgct cttcttcagt caaatcatct gccaaccaac cgatcaccac actgtaattg
    22801 cccgtgcgta aagcgcgaac cattgactcc acagtgtggc aaggggagag ctggctaatc
    22861 tgcattactt tcgttaaggg tagcccagat gcctgaaccc attcccgact cagtttttgt
    22921 tgcggtgtta accagagttg ccagcgcgat tgctgaccga gttgctgtaa caatggcaac
    22981 agtagaagtt gcgtcatcat gggctgatct tcgcgataga caacttcact gataagcccg
    23041 gctgtagtgt tttccgtaga gacacgcgca attttacttg ctgcggatga gaacgacgaa
    23101 gaacgatgtg catagcctga agtgtacata atcaatccag cccctgtgag ttactgtatg
    23161 gatgtacagt acatccagtg acaacaaaga tcaaccctat tttcggaaag agcctcgcaa
    23221 attttgtcgt tggtgacggg aaaacataaa ttaatcttgc cgcttaagaa taagttgcct
    23281 attttcgtag ttaacggatc cgttaatgtg aatcattctt ttatgttatg attttaaaag
    23341 gaattttatg aaaagcctct cctataagcg gatctataaa tcacaagaat acctggcaac
    23401 gttgggcaca attgaatacc gatcattgtt tggcagttac agcctgaccg ttgacgacac
    23461 ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca
    23521 gtactgtgta aaatatccgc ctgtctggct gacatataaa aagtgtggcc gatccgttac
    23581 cctcaattac tatcgggttg atgaaagtct atggcgaaat caactgaagc tggtgcgtct
    23641 gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc acgcgcaata cccgggaaag
    23701 actgaaagat ttgcccaata tgtcttttca tctggaagcg attctcgggg aggtggggat
    23761 taaggatgta cgggcgttac gtatacttgg ggcaaaaatg tgttggttgc gactgcggca
    23821 gcaaaacagt ctggtgacag aaaagattct gtttatgctt gaaggtgcca ttatcggcat
    23881 tcatgaagct gcgctcccgg tggcacgccg ccaggagctt gcagaatggg ctgactctct
    23941 tacgccgaaa caggagtttc ctgcggaact tgagtaatct ggcgttgcag acgaccaatc
    24001 tcaggcagta atgcaatcaa taatccaact tgttgtacga ccagaggttc tttgctgtct
    24061 gcccgtggtt caagttgctg catccgctgt ttcaggctag ctaatgcctc attgacgcgt
    24121 tcttcatcag caggttgatg atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga
    24181 aacgccagaa tttcaggatt agttaactgc tcccggtgag caccgagggc tgagatatag
    24241 ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta
    24301 acgttcggct cgctggacat atttgatacc accgacgcca gctcagcatc acggttgtgt
    24361 gcatcgcggc gggcaatacg atacgccaga cggttatcac gcccctgatg gtattgctcc
    24421 agtatggcat cgagataccg acagttggcc tctgtggcgc gttcgagcat gcgcggcaga
    24481 ttgcgaaact gccagtcagg ccagatgtag ctcactgccg cccacgcaat ggcacaacca
    24541 atcagcgtat cgattacgcg aggtaacgct acttcaaaac cttcacccag taagttaaaa
    24601 cacagtagca ccaaaagtgt gatgaacatc gttgcatgag cgtattgcac gttacggaag
    24661 gcaaaaaaga gcacgccggt aataaccagc agcaccagct gcccttccag tgatggcaca
    24721 aaccacagca caggaatgcc aatggcgata cctaccagcg taccaataat ccttaacttc
    24781 aggcggtggc gcgtggcgtt atagtttggc tggcagacaa acaaacttgt cagcaagatc
    24841 caatacccgt gatgcattcc ggttatctga atgatggcgt agccgaagca caacaccagc
    24901 gacattctta ccgcatgacg gaagagggcg gattccggcg tgaagtgacg gctaagacgc
    24961 agccagatat cactcaaccc gtgcgggctg tcatcagcga gctcattttc gtcattatta
    25021 tggggtagtg cctgggcctg ttctgattca attgttgcca gttgggcatc aatggcgcgt
    25081 aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg cgggtgcgcc gttatcgcgc
    25141 atccgctcca gcgcagcatc aatatgcgta aaagcgcgct caaaatgcgg atcatgttga
    25201 taaggctgac gcaacaaaat acagcgtgac agttgctggc acgcctggcc ctgcatcgac
    25261 atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa aatgttcacg caatgtttga
    25321 tactgaatat gagaagagct ggcacgctcg tgaatatcct gtgcgacaaa gtaataatgc
    25381 agcgtgcgac gcgttccccg ttgaccacga tcgccacgta agcgggtcag cagcgagagt
    25441 ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga gagccaaatc gtacagcggt
    25501 gcctggcttt catcttcaat atcaggatca aacatgcgcg acttgagctc aagataacgc
    25561 gccagttgtt catagcaacg cgccaggttg tcctgcagcg ggcggaccgg gaacagcaga
    25621 tgaccaataa gtgtcaggac gttgtaccag acggcaccgg ccagcagata catcggctgc
    25681 tgataccagt gctcatacag tgatgttccc aacatagtgt aaatggcgat cagcaatgca
    25741 ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga gcaaaatgaa gccgctggta
    25801 gagagcgtta agccaatcgc aaatagccag ggccagggaa acagcaattc tactgaggcc
    25861 gaggcgataa aaaagcagaa cagcgtaatg atgaggttac gcaaacgtcc cgccagtcgg
    25921 tcatcgagat cggtcagcgc cgctgccacc atccccagcg ttagcggaat cgtcagtttt
    25981 acatcaccca gccaccacgg aaacgctgtg gttccacaaa gcgcaataaa aatacgcgcg
    26041 taatacagcc aggcgctgtt ccaggtatag cgtttgagca aaggacttag cataaaggcc
    26101 atattagtgg attattcaaa acgacgacgt gcattggctt cacgcgcagc ttgcgctgtt
    26161 tctaccgata ccacgcgacg accaaccggc catagtgcaa tagcggcaat tttaaagttc
    26221 gcaatgccga caggaatgcc aatgattgaa atacattgtg cgatgcccgt tgcaatgtgc
    26281 atcaggcata accaccagcc aaagaaaatc agccagaaaa tattcaatac cgtaccgcca
    26341 gtattcagca gcacattttt gccagccggg ttcagttcat cgacatgaat agcttcattg
    26401 ccataaggca ccagagacag tttagtgatc tcccagcagg atcgtgtcag cggtaaggta
    26461 aaaatcagcg caatactgac cagagtcgcc aacagccagc ccagagtggt ggcaaatccg
    26521 ccaagcacaa agttcagaat gttcaaaacg gtacgcataa aacctcgctt tactgtggtt
    26581 ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg agcacgtttt ctctgacggt
    26641 tacactgata agaaataatt tcgtgtggat ctacagagtc atggaactga aagcgacaac
    26701 gcttggaaaa cgtctggcac agcaccctta cgatcgggcg gtgatcctca atgccgggat
    26761 taaagtctcc ggcgatcgcc acgaatacct tattcctttc aatcaattac tggcgattca
    26821 ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta ctgccggacg aaaaagtggt
    26881 gcgtctgcac ggtaccgaat ggggcgagac gcagcgtttt taccatcatc ttgatgctca
    26941 ctggcggcgg tggagtggcg agatgagcga aattgcgtct ggtgttttac gccagcaact
    27001 ggatttgatt gccacgcgca ctggagaaaa taaatggctg acgcgtgagc aaacctctgg
    27061 cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg ttgccggtta accgactgga
    27121 agaattcgat aactgccgtg aggcgtggcg taaatgtcag gcctggttga aagatattga
    27181 aagcgctcgg ttgcagcata accaggcgta taccgaagcc atgcttaccg agtatgcgga
    27241 ttttttccgc caggtcgagt cttcaccgct gaatccggcg caggcccggg cagtcgttaa
    27301 tggcgagcat tctctgttag tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt
    27361 ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct
    27421 ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgt attcgcgaac ggctacatac
    27481 cgaagacatt accgcacgca cgtttcatgc gctggcgctg catattattc agcagggcag
    27541 caaaaaagtt ccgatagtca gcaaactgga aaatgatacc gctgcccgtc atgaactctt
    27601 tattgctgag tggcgcaagc aatgcagcga aaagaaagcg caggcgaagg gctggcggca
    27661 atggctgacg gaagaaatgc agtggtcagt gccagaaggt aacttctggg atgatgaaaa
    27721 attacagcgt cgtcttgcct cacgcctcga tcgttgggta agtctgatgc ggatgcacgg
    27781 tggtgcacag gcagaaatga ttgccagtgc acccgaagag attcgcgatc tgttcagtaa
    27841 acgtatcaag ttgatggccc cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa
    27901 cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg attctggaga aaggtcgctt
    27961 tatcagcccg tggaagcata ttctggttga tgaatttcag gatatctcgc cgcagcgggc
    28021 agcgttgtta gcggcattac gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga
    28081 tgactggcag gcgatttacc gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca
    28141 tgaaaacttt ggtgaaggcg aacgctgtga tttagacacg acttaccgtt ttaacagtcg
    28201 tatcggtgag gtggcaaacc ggtttattca gcagaaccca ggccagctga aaaagccgct
    28261 aaacagctta accaatggag acaaaaaagc cgtcacgtta ttggatgaga gtcaacttga
    28321 cgctttgctg gataagctct ctggttatgc caaaccggaa gagcgcattc tgatcctggc
    28381 gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt
    28441 gcaaatcgac tttatgacca ttcatgccag caaagggcaa caggcggatt acgtcctcat
    28501 cgttggcttg caggagggaa gtgatggttt tccggctgcg gcgcgggagt cgattatgga
    28561 agaggcgcta ctgccaccgg ttgaggattt cccggacgct gaagaacggc ggttaatgta
    28621 cgtggcgctg acccgggcac gtcatcgggt atgggcactg tttaacaaag agaatccctc
    28681 tccctttgtg gaaatactga aaaatctgga tgtgccggtg gcgagaaaac cgtaagaaac
    28741 aggtggcgtt tgccacctgt gcaatattac ttcagacggt ccgcgagata acgctgataa
    28801 tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg actggataat gaagtctgcc
    28861 gttgccacgt tggtggcgac cggaatgttc cataccgtcg ccagacgcag caaggctttc
    28921 acgtcaggat cgtgcggcac ggcatttagt ggatcccaga agaaaatcaa tacatcaatt
    28981 ttcccttctg agatcaatgc gccaacctgc tggtcacccc ccattgggcc actcaacatc
    29041 gcgttgacgt tcatgccggt cgcgcgggaa attaagttac cggtagtgcc tgttgcatac
    29101 agtacgtgtt gttccagtaa cggttgatgc cgttccaccc agctcatcag catttgtttg
    29161 cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt
    29221 tccataatgt acatccgtag ttaactttcc tacagattac tgtaagcact tatcgctgca
    29281 agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt
    29341 ccgcccattg caggaaagta tttcgcgttt gtgggtcagc cagtttaaac caatacttca
    29401 gccgttgttc tgtgagcacc tgagactgcg gtggaatagc atccagctcg gaaacacccg
    29461 aaagcagcgt actgtcatct gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg
    29521 cggatttgtt atagcgttca acctcgacct cataatcaat cgtttttgcc gttgaggtaa
    29581 tggcgagaat atccagcttt accggaatcg gcgtcgcatc gccatccaac aattcaaggc
    29641 gcggcgcggc atcaaaatgg ttagcttccc gctcattctc caggcgaggc aggcgaaaat
    29701 tgacctggtt gatgagctgg gtattaaaac tgaccaccag cggtggggag atatacagcc
    29761 gttcttcact attggaaaga tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc
    29821 cattatccag ttcaatgctg tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt
    29881 cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg
    29941 gcaaacataa tgcaatcaag gtggtcacga tgccggtttt cataggtagc tcctgtcaaa
    30001 agaccgcccg aataataatg tgtcaaaatt tttctttaat catacttact aacatataga
    30061 catatttccc gcttttgctc tcattcattc gtattagctg catggttggc atgtcggctt
    30121 tgtcgtacac tttgtgaaac agtctggagg aaataatgaa agaaaccgat attgcaggca
    30181 ttttaacgtc cactcacacc attgcgctgg tgggggcaag cgacaaaccc gatcgtccca
    30241 gctatcgcgt gatgaaatat ctactcgacc aggggtatca cgtcattccg gtttcgccaa
    30301 aagttgccgg caaaacgctg ctgggccagc aggggtacgg cacgctggca gacgttccgg
    30361 aaaaagtcga tatggtcgat gttttccgta attcagaagc ggcgtggggc gtagcgcagg
    30421 aagctatcgc cattggcgcg aaaaccctct ggatgcaact gggggtgatt aatgagcaag
    30481 cggcagtact ggcacgggat gccgggttga atgtggtgat ggatcgctgc ccggctattg
    30541 agatccctcg cctgggcctg gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg
    30601 atctgttcag ggattagtta cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg
    30661 ccagctcatc catggatggc tgttcaggat gttcatcctg caattcgcta cttagctggg
    30721 cttcagccag gtaggtatga accggtaggc cgttatcgtc ctccatcacc acgtgatacc
    30781 acggagcggc gcgaagctcg tcattcaccg ccagctcatc aggcgacggt tcagagagcg
    30841 aataaaccgg gtcgatatcc acgaccactc cgagataacc taacagggaa tggcggacct
    30901 gctggccgat accgaatttg ctggcaatca tagtcacctc ccgggaaatc tttacctttg
    30961 atataagggt aaaattccac atttcaagtt acatgacgcg acaggcaaac cctttcagat
    31021 atagcccttc cggataggta gcgatcaccg gatgatcggc cgcctgacgg aactgctcta
    31081 taaattgtac atcacgaccg gcatcaattg cggcatccgc gatgattttc tgaaataaat
    31141 cgctggtcat cagaccggaa caggagaaag tcaggagaat accgccttca ttcagcagct
    31201 gaatcgccag catgttgata tctttatagc cacgacacgc gcccatcaac tggcttttat
    31261 tctcaacaaa cttcggcggg tccatcacga taacgtcaaa tttttcaccg cgatcgcgat
    31321 aggtacgcag caatttaaag acatcatcac ggacaaactc agccttgctc agatccagtt
    31381 tgttcagctc aacgttctgc cgtgcaatat ccagtgcttc ctgggaggta tcaacgctga
    31441 caacctggct gcaaccgccc atcagtgccg atacggcgaa accaccggta taggagaaac
    31501 agttcagtac gcgtttattt tcaacgtagc ggcgggtagc caggcggctg tctcgctggt
    31561 ccaggtagta gcccgttttg tgtccgtgct gtatgtccac cagcagcttc atgccatgtt
    31621 cttcaatcgg cagcagggca ggcggcaact caccggtgac gaggccctgg gtcagctcca
    31681 tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta aatcgcacat tccgggtaca
    31741 gcgtttgcag ggcactaatt aatgccgcgc gctgatattc tgcgccagca ctcagcagtt
    31801 gcagcaccag aaaattaccg aaacgatcga tagtaatacc cggcaggcca tcagattctc
    31861 cggcgattaa acgatagctg tcgaggccat ctttttgcgc cagccagtca cgccattttt
    31921 gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat agactcagac gggtcaaacg
    31981 tccagacgcg cgcccggatt tgcgaagctg gcgaataagc gccgcgtgct aaccattttc
    32041 cctgatgatc aacaatatcg atggtttcac cgaggctggc tttaccttcc atacgggcaa
    32101 cggccccgga aaagacccac ggatggcgac gaagtaatga tttttcgcgc cctttggcta
    32161 acactaaacg tacactcata atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt
    32221 ccggagtttt ccatggattt gcaacgcgtc cattaaggat aaggagagca gcaatgtcga
    32281 aagtctgcat aattgcttgg gtttacgggc gagttcaggg cgtaggattt cgctacacca
    32341 cacagtacga agcgaaaaga ctgggcttaa ccgggtacgc caaaaatctt gacgatggca
    32401 gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtaga aaaattaatg cagtggttaa
    32461 agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct cagcgagccg catcatccct
    32521 cgggggaatt aaccgatttt cgtatccgtt aaatacattt taccggtttt ggcaggccag
    32581 caattttggt ggcttgctta gccggacctt tcggaaacag tcggtataaa tagcggctgt
    32641 tacctttttc ttcgccaaat ttattcgcca tcgcttttac cagcatacga atcgccggag
    32701 aagtattgaa ttccagatag aaatcacgca caaaacgcac cacttcccag tgttctggcg
    32761 acagcgaaat cccttcgttc tctgcaatca ccactgccag cggctcactc cactggctgc
    32821 tttctttgag atagccttcg gtatccgttt ctatctcttt accttcgaag atcagcataa
    32881 ttattactac cttaatcaga ctgccggcag tgtaacaaaa aacaaagccc cgcataaagc
    32941 gaggctatga aagtgttagc gggtgagatt aatcgcggct ggcgaagccc agaatgctca
    33001 gcaggctgac gaagatgttg tacagcgaaa catacaggct aaccgtggca cgaatatagt
    33061 tcgtttcacc gccatgaatg atgttgctgg tttcaaacaa aatagcgcca gaggagatca
    33121 aaatgaagac cgcgctgatc gccagatgca gagcaggcag ctgcaggaag atattagcaa
    33181 ccataccaat cagcaccacc acaatacccg ccatcagcat accgccgagg aacgacatat
    33241 ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa cactaacgcc gttccgccca
    33301 gtgccatagc gattacgtca cccattccgg cagacagata ggtattcaga attggtccga
    33361 ggatataacc cagaaaaccg gtaaaggcga atgcggagat aatcccggtc ggcttattcg
    33421 ccattttata ggtcaggaac atcaaaccat acatacccac cagcgtcaga atcagacccg
    33481 gagatggcag catcagcacc gtactggcag ttgcggtaat cgccgaaaag gccagcgtca
    33541 ggctcagcag aaaataggta ttacgcagca ctttatgggt gctaagcagt gatgtacggt
    33601 catgtgaaga actaacaata cgatccattg agtcactctc tatgacagat gtaattaatt
    33661 aagcagcata atgataatgc gtaagggcac ccagtagttt tacccatctt tacgcatttg
    33721 atctggaaca ggtttaacag cggattatca ggtcattaag caaatataac gccctgagaa
    33781 tttcggcagg caaaagaaaa aggggttagc atttagctaa ccccttatct tatctggcgg
    33841 aagcgcagag attcgaactc tggaaccctt tcgggtcgcc ggttttcaag accgaagaaa
    33901 aacaaataaa aatcagtgta atacgtgaaa ttgctgaaaa gttaatcaaa tttaacacca
    33961 agaaaaatca aggtattagg aagttgctca tagctagtat accaagtctt tttgccagaa
    34021 atttttctgg attgtctact tgctaaccac gagcggcaaa tccagtgttg gtgtgatttt
    34081 tgttttacga tcgtaaatca acacctgatt ttctgtttta tgcccgctga aaatttgttt
    34141 gtcgcgacta ctgccttcgt aatctgagat ccccttggct tttatgtcgt gaaaattgca
    34201 cccgaacgga acgccaactt tttgctcggc tgcgcgttta gcctgattcc accagttatt
    34261 cagcgtttta gctatgactt tcccgccttt ggttgtattg atcacatatt cgcatgtaca
    34321 ggaagataca tttcgggcta actggatcgc tgtacgtaat cgcggtgacc attccttgat
    34381 ttgtttggtg ccggttttgt tttgctcaat gtagatccct ttatccataa tatcctgcca
    34441 tttcaactca agcacatcac cgagtcttgc cgcacagaga taggaaatct ccattgcaat
    34501 gcgtaactgt ggaattgctt ccgcatatat cgccgcatac tgttcatcgg tgatgtaaac
    34561 agtgcgggct ttaagagaga attttctgac tcctttgcat gggttattct tcacataccc
    34621 acgctcatat ccccacccgt atactcgact caggcttgcc agttcatgat ttgcctgggt
    34681 cttgctctca agccctcgtt tatccatgaa aattcttacc tgctcagttt tgacattatc
    34741 agcaagcact tttccgaata ccatcagcaa cgccttctga tgttgtcgat aatctttttg
    34801 ggttcggggg gccagttctg taaatgcagg ggagtccata aacatgtgcc acaatttagc
    34861 tacggtcatt atgttgtgga gttttgcttt ttccagttca taattttgcc agactttagc
    34921 tacgctggtt ttccgcactc ttcctagccc tatacttctt gtacttcctt cgggtttcca
    34981 cacgtaactg taaccattcg atctaacccg cggtggcagt acattatctt ttttattttt
    35041 tcttggtctt cccattgttc agcgcctcaa aatcgggttc agcagaaacc agttcagatg
    35101 tttttggcat cgttgtcaga ccgtgtggaa tatccctgcg gagaactatg ggttcgtttt
    35161 tagggccgat tacaaatggg atgccgtgca gtcttaactg gtgttgctgt tttgtgtatc
    35221 gctcgtattt cgtgatctct ttaatctctg ctggagatag agttaattcg tacatgtggt
    35281 cacgtgcctt tacagcatga ccgccgccac tatagctggt gggcggcgat atgggttgaa
    35341 catcaaaaat cagcctgact cgggagcagt ttttgccaga tggctgaaac gtatttcgcc
    35401 tggtaacg
//
LOCUS       DAZGXS010000033        35211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGXS010000033 DAZGXS010000000
VERSION     DAZGXS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(288..1229)
                     /gene="fabY"
                     /locus_tag="ACHKG5_001794"
                     /gene_synonym="yiiD"
     CDS             complement(288..1229)
                     /gene="fabY"
                     /locus_tag="ACHKG5_001794"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0867911.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1274..1711)
                     /gene="dtd"
                     /locus_tag="ACHKG5_001795"
     CDS             complement(1274..1711)
                     /gene="dtd"
                     /locus_tag="ACHKG5_001795"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0867912.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1708..2580)
                     /gene="yihY"
                     /locus_tag="ACHKG5_001796"
     CDS             complement(1708..2580)
                     /gene="yihY"
                     /locus_tag="ACHKG5_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0867913.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2574..3173)
                     /gene="yihX"
                     /locus_tag="ACHKG5_001797"
     CDS             complement(2574..3173)
                     /gene="yihX"
                     /locus_tag="ACHKG5_001797"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0867914.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3272..4057)
                     /gene="csqR"
                     /locus_tag="ACHKG5_001798"
     CDS             complement(3272..4057)
                     /gene="csqR"
                     /locus_tag="ACHKG5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0867915.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4091..4987)
                     /gene="yihV"
                     /locus_tag="ACHKG5_001799"
     CDS             complement(4091..4987)
                     /gene="yihV"
                     /locus_tag="ACHKG5_001799"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP0867916.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5155..6051
                     /gene="yihU"
                     /locus_tag="ACHKG5_001800"
     CDS             5155..6051
                     /gene="yihU"
                     /locus_tag="ACHKG5_001800"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0867917.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6075..6953
                     /gene="yihT"
                     /locus_tag="ACHKG5_001801"
     CDS             6075..6953
                     /gene="yihT"
                     /locus_tag="ACHKG5_001801"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP0867918.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6969..8210
                     /gene="yihS"
                     /locus_tag="ACHKG5_001802"
     CDS             6969..8210
                     /gene="yihS"
                     /locus_tag="ACHKG5_001802"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP0867919.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8277..9179
                     /gene="yihR"
                     /locus_tag="ACHKG5_001803"
     CDS             8277..9179
                     /gene="yihR"
                     /locus_tag="ACHKG5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP0867920.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9240..10115
                     /locus_tag="ACHKG5_001804"
     CDS             9240..10115
                     /locus_tag="ACHKG5_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0867921.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10223..12259
                     /gene="yihQ"
                     /locus_tag="ACHKG5_001805"
     CDS             10223..12259
                     /gene="yihQ"
                     /locus_tag="ACHKG5_001805"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP0867922.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12305..13690
                     /gene="yihP"
                     /locus_tag="ACHKG5_001806"
     CDS             12305..13690
                     /gene="yihP"
                     /locus_tag="ACHKG5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867923.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLRLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13733..15136
                     /gene="yihO"
                     /locus_tag="ACHKG5_001807"
     CDS             13733..15136
                     /gene="yihO"
                     /locus_tag="ACHKG5_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867924.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15203..15895
                     /gene="ompL"
                     /locus_tag="ACHKG5_001808"
     CDS             15203..15895
                     /gene="ompL"
                     /locus_tag="ACHKG5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP0867925.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15986..17251)
                     /gene="yihN"
                     /locus_tag="ACHKG5_001809"
     CDS             complement(15986..17251)
                     /gene="yihN"
                     /locus_tag="ACHKG5_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867926.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17353..18333)
                     /gene="yihM"
                     /locus_tag="ACHKG5_001810"
                     /pseudo
     CDS             complement(17353..18333)
                     /gene="yihM"
                     /locus_tag="ACHKG5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(18341..19051)
                     /gene="yihL"
                     /locus_tag="ACHKG5_001811"
     CDS             complement(18341..19051)
                     /gene="yihL"
                     /locus_tag="ACHKG5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0867927.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19268..21091)
                     /gene="typA"
                     /locus_tag="ACHKG5_001812"
     CDS             complement(19268..21091)
                     /gene="typA"
                     /locus_tag="ACHKG5_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0867928.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21464..22873
                     /gene="glnA"
                     /locus_tag="ACHKG5_001813"
     CDS             21464..22873
                     /gene="glnA"
                     /locus_tag="ACHKG5_001813"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0867929.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            23047..24096
                     /gene="glnL"
                     /locus_tag="ACHKG5_001814"
     CDS             23047..24096
                     /gene="glnL"
                     /locus_tag="ACHKG5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0867930.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            24108..25517
                     /gene="glnG"
                     /locus_tag="ACHKG5_001815"
     CDS             24108..25517
                     /gene="glnG"
                     /locus_tag="ACHKG5_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0867931.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25629..25739
                     /gene="yshB"
                     /locus_tag="ACHKG5_001816"
     CDS             25629..25739
                     /gene="yshB"
                     /locus_tag="ACHKG5_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0867932.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25929..27302)
                     /gene="hemN"
                     /locus_tag="ACHKG5_001817"
     CDS             complement(25929..27302)
                     /gene="hemN"
                     /locus_tag="ACHKG5_001817"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0867933.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27491..27997)
                     /gene="yihI"
                     /locus_tag="ACHKG5_001818"
     CDS             complement(27491..27997)
                     /gene="yihI"
                     /locus_tag="ACHKG5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0867934.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(28116..28282)
                     /locus_tag="ACHKG5_001819"
                     /pseudo
     CDS             complement(28116..28282)
                     /locus_tag="ACHKG5_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28579..29211
                     /gene="yihA"
                     /locus_tag="ACHKG5_001820"
     CDS             28579..29211
                     /gene="yihA"
                     /locus_tag="ACHKG5_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0867935.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29593..32379)
                     /gene="polA"
                     /locus_tag="ACHKG5_001821"
     CDS             complement(29593..32379)
                     /gene="polA"
                     /locus_tag="ACHKG5_001821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0867936.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32743..33675
                     /gene="yihG"
                     /locus_tag="ACHKG5_001822"
     CDS             32743..33675
                     /gene="yihG"
                     /locus_tag="ACHKG5_001822"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0867937.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(33716..35146)
                     /gene="yihF"
                     /locus_tag="ACHKG5_001823"
     CDS             complement(33716..35146)
                     /gene="yihF"
                     /locus_tag="ACHKG5_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0867938.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8590 a         8977 c         8591 g         9053 t
ORIGIN      
        1 tatagatttt taaagtattt atgagaaaac tatggcacca gagggggttt tgactatcaa
       61 aaatcgctca aacctgaact aagatgatga attaagcatt atctgcgata attgctatac
      121 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggagc
      181 caacgaagaa taaaaattta tagagttctt attcaatata accgaaatag ccaaggacta
      241 atataaatga cctgaatgag agaggcagca ccgcccctct ccatcagcta ctcttcttcg
      301 ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata cgtgccttca
      361 aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac ccgtgctttt
      421 cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc taccgcatgg
      481 ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat aatcgttccg
      541 ccgaggtggc gttcgcgcag catcagccag ataagccccc aaccggtgag cgtcgccagt
      601 gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt ttctggcatg
      661 gtagtgataa atttttgccc ggtatattgc tgaatgcgca cgcccatttt ttcactaagc
      721 gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg atgcaaaata
      781 tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt ggtggttggc
      841 gtggtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac cgcgtcttca
      901 cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc cagggtcatc
      961 gccatcagcg tgcctaaccc tttgtcctgc acgtcgggat gaacggccat aaagcgaatg
     1021 gacgcttcat tgtcggcatt aatatacagt cggcctaccg ccaccagatt accctgctcg
     1081 tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga accttttggt
     1141 tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc taattcttct
     1201 tctgtttgtg gaacccgaag gtgatacata gcgatactct ctcttgtttc ccgtgacaac
     1261 cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg accagcgata
     1321 cctgcatatc cgcagcgaag cgtcctgttt gcgtgttcat ctcttgctga cggcagcgtt
     1381 cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg gagaaacttg
     1441 ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc agcacactgc
     1501 cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag atgcggtagc
     1561 cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt tcgacaccca
     1621 ataacaccaa aagtcccgca ccaatttcgc ccgtcacttc tccctccacg gtgacgctgg
     1681 cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt gttcagctgc
     1741 ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa gcaagacgat
     1801 acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca caccgtaaat
     1861 gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc ctgcttcgaa
     1921 caggagtgca gcaacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc ggatggtagg
     1981 aacaatgctg tacagcaacc agaaggagat ccacgacaac agcaacggaa aaatacgcag
     2041 cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca aataggaact
     2101 gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc agtacacggc
     2161 aaacgagtaa attttgggtc gcgctcgttt actgcgccag atggtattca acgcgctatc
     2221 gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa cggcggtcat
     2281 cttgttggaa ttggcaacaa attgttcgat ataccgctga ataacatcgc cagtagcagg
     2341 cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa acatgggaaa
     2401 agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca acgacacata
     2461 ggcaaggtta cctgccaggg ttgtcatatt gtcctcatca atgcgttgcc agagtagttt
     2521 tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg tttttagcat
     2581 aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat actggtaatg
     2641 cccagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac cgtatcgctg
     2701 ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gagcttcagg tttgcgcatc
     2761 cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg gtattcttcc
     2821 ggccagaagg tggtatgcag gcggttagta ttggaaagca ccaccacgcg atgcccctgc
     2881 tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa caccgcctgc
     2941 cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca cagcgcctct
     3001 gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc ccccatatga
     3061 aaactcttct taagcgtagc cagcggaata cgcgttaaat cgctccaggt tcccagcaca
     3121 cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag catttttgcc
     3181 tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc tatgtggcaa
     3241 ggtatgaagc caggtcaaca gtcacaaaaa tctacgcgtc ttcctgggga aggatgattt
     3301 caatttggct gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg tcggtaaaga
     3361 gcgcggtgac ctgtgccacg ttaccaattt caaccgccgc cgaagcatga tacttagtgt
     3421 gatcggcaac cagcaggata tttctcgcgt gcgccatcat cgttttcacc acgttagctt
     3481 cgtttacatc aaactccatc aacgcgccat cgctctcaat cgccccaacg cttgttacca
     3541 gataatcagc acgaaaatcg gccacaaagg acgccgctga agggccaatg atcccgctat
     3601 tatgagagcg caacgtaccg ccgggtacca tcacttcaaa gcgcgggttg tggtaaagaa
     3661 tatgcgccac acgcaggctg ttggtgatta tccgcaaatg attatggtta agtaacgccc
     3721 gggcaacatg ctcaacggtc gtaccaatgg tgataaatat tgttgaacca tcaggaatat
     3781 agtctgccac cgcttcggca atcgcttttt tttcctcggt ttgcgaaacc tcacgctgct
     3841 cgaacgccgt attaacgacg ctggaagccc gacccgcgcc accgtgatgg cgcgtaatca
     3901 ggccttgctc gcttaattta cgaatatccc ggcgcaccgt ctgcgtggag acatccagca
     3961 gatttgccag ctcatcaata ttcatatacc cacgctcggc gatcagcatg aggagttggt
     4021 cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa aattcctcga aaaaccatca
     4081 ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt ttgatcacag tcagggatcc
     4141 cggctcgtcc accgggacgc gtgcatttcg acgccgctac accgctggcg aagcggactg
     4201 actccgctaa atccccacct gttgccagca ccaccgccaa tgcgccgtga aaaacatcac
     4261 ccgcacctgt ggtatctacc acatcaactt tgaaggccgg ttgatgttga cgcccgccat
     4321 tttccagcca gtcacagcct gcgctgccct gggtcacata gacatgacca tttgtgagcg
     4381 tttgtgcctg ttttagcgca ctggccatct ctttcacgcc cgttaagcgc gccagacccg
     4441 gttctgaaaa ggccgcatga tcgcttaatg ccaccagctc gctgatatcc tgcggcgtaa
     4501 tatcaccgtc cagaacggtc atcacgcccg cctgccgggc cagagtgaag gcttttttag
     4561 cgccgtcgtg ccagcgtaca tctgccagca caacatccca ctgagagaaa tcaatttcct
     4621 ccaaccactc tgcgtcaggc agcaggtccg ggctggggta gttaatgatt atccgctcgc
     4681 ctttggcatc caccatgatg gcggattgcg acgatttcgc ctgtttatac cgtttggtgt
     4741 agcgggtgtt aacgccccag gattccagct ctgccagcag gctgttgccg gtgtcgtcat
     4801 caccgacgcg cccaataaaa tcaacctgcg ctcccagtct tgccgccgca accgctgccg
     4861 tcgccgctgg cccgccacca acttccgtat aatttctcgc cacgtattta ccgctctccg
     4921 tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt tatacctaca caagcaacac
     4981 gaatcatggt cattccttaa acattttggg atgcgttcat tttaatttca ccaattgttt
     5041 aaaaagtgac ggctgtcaat tttttgacta taaattacaa atacgatcaa aaacagacaa
     5101 tcaaagcgtg tcataaatga caaaaagtga catcactgta ttcaggagaa ggttatggca
     5161 gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa tggcgagcaa tttattgcag
     5221 caagggcacc aacttcgcgt ctttgatgtg aatgccgagg ctgtgcggca tctggtagac
     5281 aaaggcgcga ctcccgccgc caacccggcg caggccgcta aagatgccga atttatcatt
     5341 accatgttgc cgaatggcga tctggtgcgc agcgtgttgt tcggtgaaaa cggcgtttgc
     5401 gaaagcttat ctaccgatgc gctggtcatt gatatgtcga ccatccatcc gctgcaaacc
     5461 gataaattga ttgccgatat gcaagccaaa ggcttcaaca tgatggatgt tccggtaggc
     5521 cgtacttctg caaatgccat taccggtact ctgttactgc tggctggcgg caccgctgaa
     5581 caagttgaac gtgccacgcc gatcctgatg gcgatgggca gtgagttgat caacgcaggc
     5641 ggtccgggca tggggatccg cgttaagctc atcaacaact atatgagcat cgcgctcaat
     5701 gcgctttcgg cagaagctgc cgttttgtgc gaagccctga atcttccctt cgatgttgcc
     5761 gtcaaagtga tgagcggtac cgccgccggt aaaggccact tcaccacttc ctggccgaac
     5821 aaagtcctca gcggggatct ttctcccgcc ttcatgatcg atcttgccca taaggatctt
     5881 ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa tgccgctggg ggccgcctca
     5941 cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc gccaggactg gtccgccatt
     6001 ctggaacagg tccgtgtcag tgccgggatg actgccaaag taaaaatgta acgactggat
     6061 aaaggaataa atgaatgaat aagtacacca tcaacgacat tacgcgcgca tcgggcggtt
     6121 ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat gatgtttgcc gcggctgggg
     6181 cacccgctcc ggtagccgat agcgttttaa ctgatttcaa agttaacgcg gcgaagaccc
     6241 tctcgcctta tgcctcggcg attctggtag atcaacaatt ctgttaccgc caggtggttg
     6301 agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc cgccgatgag ttcattcctg
     6361 gcaacggtat tccggtagat agcgtggtca ttgaccgcaa aatcaatccg ctacagatca
     6421 aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg gcgtagtgat gaagatgcgc
     6481 agcaacgcct ggatatggtg aaagagttta acgaactgtg ccactcacac ggcctggtaa
     6541 gcattattga gccagtcgtc cgcccaccac gtcgtggcga taaattcgat cgcgaacaag
     6601 cgatcatcga tgccgccaaa gagctgggcg acagtggcgc tgacctctac aaagttgaaa
     6661 tgccgcttta cggtaaaggt ccgcaacaag agcttctcag tgcttcacaa cgtctgaatg
     6721 accatatcaa tatgccgtgg gtgatcctct cttccggtgt cgacgaaaaa ctgttcccgc
     6781 gtgccgtacg cgtggcaatg acagcgggtg catcgggctt cctggctggt cgtgctgtct
     6841 gggcatcggt cgtcggttta ccagacaacg agctgatgct gcgtgacgtt tgcgcaccga
     6901 aattacaaca acttggcgat atcgtcgacg aaatgatggc taaacgccgt taaaatgggg
     6961 atacaaaaat gaaatggttt aacactttaa gtcacaaccg ttggctggaa caggaaaccg
     7021 atcgcatctt tgattttggt aaaaattccg tagtaccgac cggatttggc tggttaggca
     7081 ataaagggca aatcaaagaa gagatgggca cccatctgtg gatcacagct cgtatgttgc
     7141 acgtttattc cgttgctgcg gcgatgggtc gacctggcgc ttactcgttg gttgatcacg
     7201 gtattaaagc catgaacggc gcactgcgcg ataaaaaata tggcggctgg tatgcctgtg
     7261 tgaatgacga gggcgtggtg gatgcctcca aacagggcta tcaacatttc tttgctctgc
     7321 tgggtgctgc cagcgccgtc acaacgggtc acccggaagc gcgcaagctg ctcgattaca
     7381 ccattgaaat tatcgagaaa tacttctgga gcgaagaaga gcagatgtgc ctggagtcct
     7441 gggacgaagc cttcagcaaa accgaagagt accgcggcgg caatgccaat atgcacgcag
     7501 tggaagcttt cttaattgtt tatgacgtaa cgcatgacaa aaaatggctg gatcgcgcga
     7561 ttcgcgtggc ttccgtgatt atccacgacg tcgcaagaaa taatcattat cgcgttaacg
     7621 aacacttcga tacccagtgg aatccgctgc cggattacaa caaagataac ccggcgcacc
     7681 gcttccgcgc gttcggaggt acaccaggcc actggatcga atggggccgt ttaatgctgc
     7741 acatccacgc ggccctggaa gcccgttgcg aacaaccgcc agcatggctg ctagaagatg
     7801 ccaaaggtct gtttaacgcc accgtgcgtg atgcctgggc acccgatggt gcggacggga
     7861 ttgtttatac cgttgactgg gaaggaaaac cggtggtccg cgaacgtgtg cgttggccta
     7921 tcgtcgaagc gatgggtacg gcctacgcgc tctacaccgt caccggcgat cgccagtacg
     7981 aaacctggta tcagacatgg tgggactact gcatcaaata cctgatggac tacgaaaatg
     8041 gttcatggtg gcaggagctg gatgcggaca ataaggtcac caccaaagtc tgggacggca
     8101 aacaggatat ttatcacctg ctgcattgcc tggtgatccc gcgtatcccg ttagcgccag
     8161 gcctggctcc ggcagttgcg gcgggtctgc tggatattaa cgcgaaataa ctgactaaaa
     8221 aaacgacata tcaggctgca cgccgggtat gtcgttattc aataaaggta cctcgtatgc
     8281 aaatcaccaa catgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg
     8341 cgaccattgt gaccgtcggt gcaggtctgg ctgaattaac ctttcagggc tgtcatttag
     8401 ttattccaca taaacccgaa gagatgccgc tggcacatct gggcaaagtc cttattccgt
     8461 ggccgaaccg gatcgccaac ggctgttatc gctatcaggg gcaggaatat cagttaccca
     8521 ttaatgaaca tggttcaaaa gccgctattc atgggctgct cgcctggcgt gactggcaaa
     8581 tcagcgaatt aaccgccacc agcgttaccc tgacagcttt tctgccgccc agttacggct
     8641 atccgtttat gctggcttcg caggttgttt attcgctcaa tgcacgcacg ggtctttccg
     8701 ttgagatcgc aagtcagaat attggtactg tgaccgcacc ctatggtgtg ggtattcatc
     8761 cttatttaac ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc
     8821 aggtttatgc cgtcgatgag cacgctaatc cgacaacgct tcaccacgtt gatgagctgg
     8881 atttaaattt cacgcaggcg aaaaaaatag ccgcgacaaa aatcgaccac acctttaaaa
     8941 ccgctaatga tctgtgggaa ataaccatta cccatccaca acaggcgctt tcggttagcc
     9001 tttgttccga tcaaccctgg gtacaaattt atagcggtga aaaattacag cgtcagggac
     9061 tggcagttga acctatgagt tgcccgccta acgcctttaa ttcaggtctc gacttattat
     9121 tactggaacc agggaaaacg caccgcctgt tttttaatat ttacgggcaa cacaattaat
     9181 tttattaaga aaagaacgag atcattgttt agagttgtat ttacacaaca ggaaatatta
     9241 tggacttttt tacgttattt tcaccggatg gggtcatgct ggaaacaaac gtggtatgta
     9301 tcgttatcgc gttaatgttt ctttatacct ttgtggggat atgcgcggga tttggcggtg
     9361 gtttaaccac tatgccgctc gtcacgttaa tgctccccgt aaaaatggca acaccactct
     9421 cggtaattgt cggcaccgcg accgccttgt acgccacctg gctgtcacgt aaagaaacgg
     9481 attggcgatc ggcaatggta ctgattattt tctcgtttct gggaataccc gtcggtttat
     9541 atgcactgtc gtatttaccc gaccatttta tgaaagtggg cttaggcgga ttccttatcc
     9601 tgtactcctt ttacagtatg tttattccac gcctgccgat ttatgaccgt agctggatcg
     9661 ccgcacctat cggtgcagta gcaggcgcat taggcgcagc tttctcaacc aacggaccac
     9721 cgatggttat ctacggtatg ctgcgtaact taggcccggc ggcatttcgt ggcactctga
     9781 acgctttctt caccgcaaac aacatcgcgg tgatcggcgg gctggcgacc agcggaattt
     9841 tgactgtttc gatctttaaa ctggtgctgt tttgtattcc caccatgatc ctcggttcac
     9901 tggttggaca atatgtgcat aaacgtattt cagtcaaagt gttccgtgtg atggtctttt
     9961 tattgctgat tgcctccggt tccatgttga taaaaggcgc gctgggaatt cccgccgctg
    10021 ccgcacttct acctccgttt accttgttag ttatactgca actggtattt ggtaaaaaag
    10081 tggcggcaat ccgtcaggct gaagcaagta aataatcact tatgcgggtt taaatttata
    10141 tccgcacaca ttctttaatt aattaaacct gtaataacag gatggcatga aatattagct
    10201 ttatactcta tggagattat ttatggatac gccacgccca caattaccag attttgaatt
    10261 tcatcagaat aacgacagtt ttaccctaca ttttcgacaa cgtcttattt taacccatag
    10321 caaagataat ccttgtttat ggattggctc aggtatagcg gacatcgata tgttccgcgg
    10381 caatttcagc attaaagata aactacagga gaaaattgcg cttaccgacg ccatcgtcag
    10441 ccagtcaccg gatggttggt taatccattt cagtcgtggt tctgacatta gcgccacgct
    10501 gaatatctct gccgacgatc agggccgttt attgctggaa ctacaaaacg acaaccttaa
    10561 ccacaaccgc atctggctgc gccttgccgc tcaaccagag gaccatatct acggctgcgg
    10621 cgaacagttt tcctacttcg atctgcgcgg caaaccgttc ccgctatgga ccagtgaaca
    10681 aggcgttggt cgcaacaaac aaacctatgt cacctggcag gccgactgca aagaaaatgc
    10741 gggcggcgac tattactgga ctttcttccc acagcctacg tttgtcagca cgcagaagta
    10801 ttattgccat gttgataaca gttgctatat gaacttcgac tttagtgccc cggaatacca
    10861 tgaactggcg ctgtgggaag acaaagcaac gctgcgtttt gaatgtgctg acacatacat
    10921 ctccctgctg gaaaaattaa ccgccctgct gggacgccag ccagaactgc ccgactggat
    10981 ttatgacgga gtaacgctcg gcattcaggg cgggacggaa gtgtgccaga agaaactgga
    11041 caccatgcgt aacgcgggcg tgaaggtcaa cggcatctgg gcgcaggact ggtccggtat
    11101 tcgtatgacc tcttttggca aacgcgtgat gtggaactgg aagtggaaca gcgaaaacta
    11161 cccgcaactg gattcgcgta tcaagcagtg gaacaaagag ggcgtgcagt tccttgccta
    11221 tatcaacccg tatgttgcca gtgataaaga tctctgcgaa gaggcggcaa agcgcggtta
    11281 tctggcaaaa gatgtcgccg gtggcgacta tctggtggag tttggcgagt tctacggcgg
    11341 cgttgtcgat ctcaccaatc cggaagccta cgcctggttc aaggaagtta tcaaaaagaa
    11401 catgattgaa ctcggctgcg gcggctggat ggctgacttc ggcgagtatc tgcccaccga
    11461 cacgtacctg cataacggca tcagcgcgga aattatgcat aacgcctggc ccgcgctgtg
    11521 ggcgaaatgt aactacgaag cccttgaaga aacgggcaag ctcggcgaga tcctcttctt
    11581 tatgcgcgcc ggttctaccg gtagccagaa atactccacc atgatgtggg cgggtgacca
    11641 gaacgtggac tggagtctgg acgatggcct ggcgtcggtt gtcccggcgg cgctatctct
    11701 ggcaatgacc ggacatggcc tgcatcacag cgacattggc ggttatacca ccctgtttga
    11761 gatgaagcgc agcaaagagc tgctgctgcg ctggtgcgat ttcagcgcct tcacgccgat
    11821 gatgcgcacc cacgaaggta accgtcctgg cgacaactgg cagtttgacg gcgacgcgga
    11881 aaccatcgcc catttcgccc gtatgaccac cgtcttcacc accctgaaac cttacctgaa
    11941 agaggccgtc gcgctgaatg cgaagtccgg cctgccggtt atgcgcccgc tgttcctgca
    12001 ttacgaagac gacgcgcaga cctattccct gaaatatcag tacctgttag gtcgcgacat
    12061 tctggttgct ccggtacacg aagaaggtcg cagcgactgg acgctctatc tgccagagga
    12121 taactgggtc cacgcctgga ccggagaaac cttccacggt ggagaaataa ctgttgaagc
    12181 gcccatcggc aagccgccgg tcttttatcg cgccgacagc gaatgggctg cactgttcgc
    12241 ctcgttaaaa aacatctaat catgcccgcc cgtggggaaa ctcacgggca aatggagaat
    12301 aacgatgagt cacatcacaa cggaagatcc ggcaactttg cgcctgccct ttaaagagaa
    12361 actctcttac ggtatcggcg atctggcctc taacatcctg ctggatattg gtacgcttta
    12421 tcttttgaag ttttataccg acgttctgag gctacctggc acctatggcg gcattatctt
    12481 tttgatctcg aaattcttta ccgcctttac cgatatggga accggcatca tgttggattc
    12541 ccggcgtaag attggcccga aaggtaagtt ccgccctttc attttgtacg cgtcattccc
    12601 ggtcacctta ttggcaatcg ctaactttat cggcacaccg ttcgatgtca ctggtaaaac
    12661 ggtgatggcc actattctgt ttatgctcta cgggctgttt ttcagcatga tgaactgctc
    12721 ctatggcgcg atggtgcctg ctattaccaa aaaccccaac gagcgcgcat cactggcggc
    12781 atggcgtcag ggaggcgcta cgctgggcct gctgctgtgc acggtgggat tcgtgccggt
    12841 tatgaatctt atcgaaggta atcagcaact tggctatatc ttcgccgcca cgctgttttc
    12901 actgttcggc ctgctgttta tgtggatctg ctactcgggc gtgaaagagc gttatgtcga
    12961 aacccaacca gccaatccgg cgcaaaagcc tggcctgctg caatctttcc gcgcaattgc
    13021 cggtaaccgc ccactgttca ttctgtgcat tgccaacctc tgcactttag gggcgtttaa
    13081 cgtcaagctc gccatccagg tctattacac ccagtacgta cttaacgatc ccatcctgtt
    13141 gtcatatatg ggatttttca gcatgggctg tattttcatc ggtgtgttcc tgatgcctgg
    13201 cgcagtcagg cgttttggta agaagaaggt ctatatcggc ggcctactga tttgggtgct
    13261 gggcgatctg ctcaactatt tcttcagcgg cggttcggtc agcttcgtgg cgttctcctg
    13321 cctggcattc ttcggctcag cgtttgttaa cagcctgaac tgggcgctgg tttccgacac
    13381 cgtcgagtac ggcgagtggc gtaccggcgt tcgttcggaa ggaacggtct acaccggctt
    13441 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13501 gcaaatcggt tatgtgccga acgtggcgca ggctgaccac accattgaag ggttgcgcca
    13561 gctgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggctgctt
    13621 ctacagcctg aacgagaaga tgtatgtccg cattgttgaa gaaatagaag cccgtaaacg
    13681 cacggcgtaa taataataaa caatgccctg cggggcgtta taaggagtga ttatgtctga
    13741 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13801 cgttggttcg aatttaatgc ttagcatcgg tactctgtat ctcctcaaat tttataccga
    13861 tgaattaggg atgccagctt actatggcgg gattatcttt ctggtggcga agttttttac
    13921 cgcgtttacc gatatgctca ccggattttt actcgattcg cggaaaaata ttgggcctaa
    13981 aggcaaattt cgtcccttta ttttatacgc agcggttccg gcggcgttaa ttgcaacgct
    14041 ccagtttatc gccaccactt ttagtttgcc ggttaaaacg acgattgcca ccgcgctgtt
    14101 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    14161 cattactaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    14221 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    14281 aaccgtcggt tatgcctgtg cggcactgat gttctccatt ggtggcttta tttttatgat
    14341 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcaccaaccg gacataaagc
    14401 cagtattctc aaatcttttt gcgcgatatt tcggaatccg ccattgctgg ttttatgcat
    14461 tgctaacttg tgtaccctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14521 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14581 catcctcgtc ggcgtattac tggtgccatt aactgtaaaa tgttttggta aaaaacaggt
    14641 ttatttagct ggcatggtgc tatgggcggt tggtgatatc ctgaattatt tctggggaag
    14701 taactctttc actttcgtca tgttctcttg tatcgccttt tttggcacgg cctttgtaaa
    14761 cagcctgaac tgggcactgg tgccagatac cgtagattac ggtgaatgga aaaccggtat
    14821 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14881 tgctggcttc ttgccaggca ttatgctgac gcaaatcggt tatgttccca acatcgcgca
    14941 aagtgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    15001 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcgtt
    15061 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaag aaaaaacggc
    15121 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    15181 tacgtttaaa aatggactta ttatgaaaaa tattaatgca ataattttat tatcttctct
    15241 gacctctgcc tcagtattcg ctggcgcata cgtcgaaaac agggaggcat ataatctcgc
    15301 ctccgatcag ggcgaggtta tgctacgtgt gggttataac ttcgatatgg gcgcgggtat
    15361 tatgttaacc aatacctaca cctttcagcg agaagatgaa ctaaaacatg gatataacga
    15421 aatcgaaggt tggtatccgt tatttaaacc aaccgataaa ttaaccatcc agcccggtgg
    15481 cttaattaat gataagagta tcggttcggg tggtgctgtt tatctggatg ttaactacaa
    15541 atttactccg tggtttaacc tgacagtgcg caaccgtttt aaccataata actacagctc
    15601 aaccgattta aacggtgatc tggataataa tgacagctac gagattggca actactggaa
    15661 ctttattatt accgataagt tctcatatac ctttgagccg cattattttt ataacattaa
    15721 tgatttcaat agcggcaatg gcacgaagca tcactgggaa atcactaaca ccttccgtta
    15781 ccgcattaac gagcactggc tgccctatat tgaattgcgt tggttagatc gcaacgtgga
    15841 accgtaccac cgcgagcaaa accagattcg tatcgggacg aagtatttct tctgatgtga
    15901 aactgccgga tgcggcgtaa acgccttatc cggcctacgg ttgatacggt tttcgttggc
    15961 cggattaagt acatccggcc attaattaag cttcagccgc cagcatctct ttcttacgtt
    16021 ggctaatacg cttaaccagc attacggaaa ccaccgcacc acagaatgcg aagcaggcca
    16081 taatgccaaa cacgattttg tagccaataa tccccggatt taaatccaga atgtagccat
    16141 acagactgaa gcagaacatc gccggggcgt aaccaataaa gctacccaac gccatcgccg
    16201 cgcctgtttt attttcagca atttttgctt cgccgatagg tgcaaaaaat acggctcgct
    16261 gtgtaaagac tatcgcgcca aagcccagcg tacatgccat ccctaaatag accggcatac
    16321 tttcgtgcgg cagcataatc aacagtacga gcgcagcggt actgatgata aaggtgtagc
    16381 atagatattt actcggcgat ttcaggattt tatctgaaat catgccacca atcggtccac
    16441 caatcatttt cagacagtat tggttaatga tgccgtaagc ccccaccagc gcaacgggca
    16501 atagatagat gtttttcagg aatggaatga agaatgtcag gccacagtaa accgcgtaaa
    16561 cgaagaagac gttgaaagcg ataagccaga tagttttgtc tttcagcacc gaggtcatac
    16621 tggtgttttg cgatgccccg tcttcttttt tcacctcaac ggacggtgcc tcttctttgt
    16681 cattcaggac aaagaaaata gtaatgccta cggcaatcac aataagggaa tagaaccaga
    16741 tgcctgcttt aaaacctaat aagccactgc caaaccaggt aaatactgcc aacgcagaaa
    16801 atgccaccac ggtatcgaca atgccacgcc ctgtttcgaa gaagccaaac aaccgacctt
    16861 gttgttcgct attgcccaat cgacttaccg acttgagcaa gaccggccag ttcatcatgt
    16921 cgcaagtaac gccaaatagc gcccagacaa agaggatgcc ccaatacccc ggcattgtcg
    16981 tcaaataaac acccagtaat cctgtcgcaa tgagtgagaa tgacatggtg tatctgcgcg
    17041 gtagtttatc ggcaaaataa atagacagaa aaaagcccac tgtggtaaca aatgagttta
    17101 ccgacatagc attaccaatt tgaccattgg tcaaatggaa atattcctgc atcgggatat
    17161 aaaacgcatc tttcagcgac ggtaatttat aaattgtacc gccacacagt gttaatagac
    17221 taaataacgc ccatttcttt ttcgtgagca taacatcccc taagcgattt taaaaatcac
    17281 tgttttaaag gcaataagat accgaccatt cttatggaat ggttttatta actgcgtatg
    17341 agttaaaact tgttagtggt tcagaagatg gctcgcctct tgtttgattt gttgtagtac
    17401 gttacgcaca tgatttatct gattaattgc atcttctttt tctttacgta accgctcatc
    17461 cagcaaatgc ttttctggca atggtgtttc actcatctgg cgataaggga tccacggatt
    17521 atcatcttcg tcttcaaagc ggaacgctgg agcataataa tcaacctctt cttccaggcc
    17581 atatgccgtc acctgcggtt cttcatcgcc gaggcagata aggtgtgtta ataactcttt
    17641 gaacggcata tctccctgac ctgaaataca ggctttatga cccaggccac cttgttcttt
    17701 aacgatcaag gcatctttga tatggaccta ggtaatatgc ggtgccatcg tttttaaggc
    17761 gtctatggga tgctcatttg cattgatcat attcgcaaaa tcaaacagta aggaaagaga
    17821 ctccatctca ctttctttga ccagcgacac caactcatga ctttttaaat cttcatgctg
    17881 ttcgatcgta aaagtcaggc cgctatcctg atacgcttcc cgcacatagg caatgtcgtt
    17941 agcgatgatc gataatacgt cgcgcaaatt accttcataa cgtggataaa aacgtacgga
    18001 cgatgcccca gttttcaacg cgatggcgac ggcttcgtca atagatgcct tatctgaggc
    18061 gctggtttca atatgaatat caaggttcag acggcgggct ttatccccaa aggcagagag
    18121 ttctttatcg tccatattac caagagaaaa acgctcgcca tccagaacat ggattttgac
    18181 gccacgtaga ttattttcac ttgcgatatc aagtaaatca gcaggcaaca ctctttccag
    18241 ccgcatattt aaatgaaagg cataagcatg taaataaaga ggaagagtgt cgacacgttg
    18301 tagaatcttt cttgcgttat ttattgtcac cataaaacac ctacagatta atgatgaggt
    18361 ctacggcctc aaatcgccaa tattcaatat ctatctggac aatattgcct tgctggtcgg
    18421 cgcgatgctt ttcaattaag atcgctggca tacccgcaga accgccaaga tacttgctga
    18481 tatcgcccgg cattctgacg ggtttgaaaa tcaatttttt tactaccgtc tctttttggt
    18541 agcacttttc ccagactgca gaaaatgagt ggttttcaag ttgttcaata aaacccggag
    18601 caacttcagg attaatatat gtttcgtgat agaaaacttt atgtccttcc agcgcccccc
    18661 agccggtgat gcgatagagt tcagttgatg gtgtcactgc aatcagtggc gcgagcgtct
    18721 cgggaatatc gctggtacgg tttttctcgg taaaccccca ggaaggctct cgcccttgct
    18781 caattgcggc ccgctgaaag ctcgccgaca gctccggact gtaattaaat cgcggctggg
    18841 tcacgaacca ccccttacga tccttacgga agattttgga ttctgcctcg aggttcaaca
    18901 acgcctggcg cagcgtcata cgtttaatgc ccagcagttc tcccagctct ctttctgaag
    18961 ggagttttcc acccggcgtg gtgatgccat ctttcaacca ccgatccagt ttctcttttg
    19021 cattttctac ggtggattga ttctccgcca ttgtttaatc cttttggttt aaaccagatg
    19081 aagcatctta cgcttacgcc aaaaaagaaa gcaattaacg gcgtatgact ttgcagataa
    19141 ttgtgacaaa gctctctatt gacgtaacca cagacttata agggagatac tcgcattggt
    19201 gtgcgctcac gacaggcaaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
    19261 aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg acgatcgttt tccgtcaggt
    19321 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
    19381 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg gcttcgtcag
    19441 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
    19501 tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
    19561 acagcttacc gcgatcctgc aaaccgaaca gcgcgaacgc gaccgcttta ccctgaccgt
    19621 tagagatcag tacgccgttc tgacgctgac ccacttcacc cggacgcacg tcgtcgtagt
    19681 ggctgaaggt ggagtacagc agacccgtac cggaagtcat ggtcatgaac tcagaacgga
    19741 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
    19801 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca gaaccctgat
    19861 gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga ccgtcgattt
    19921 cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
    19981 cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca tcggcgtctt
    20041 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatccagga
    20101 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
    20161 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
    20221 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
    20281 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccaa
    20341 ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
    20401 ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
    20461 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
    20521 caatcgcctg gtacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
    20581 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
    20641 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
    20701 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
    20761 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
    20821 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
    20881 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
    20941 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
    21001 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
    21061 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
    21121 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
    21181 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
    21241 attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
    21301 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
    21361 aagttggcac agttttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
    21421 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
    21481 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
    21541 aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
    21601 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
    21661 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
    21721 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
    21781 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
    21841 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
    21901 cggttcccac gttgctatcg acgatatcga aggcgcatgg aactcctcca cccaatacga
    21961 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
    22021 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
    22081 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
    22141 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg tgcacaacgt
    22201 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
    22261 ctccggtatg cactgccaca tgtcgctgtc taaaaacggc gttaacctgt tcgcaggcga
    22321 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
    22381 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
    22441 ctacgaagct ccggtaatgc tggcttactc tgcacgcaac cgttctgcgt ctatccgtat
    22501 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg atccggcggc
    22561 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggca tcaagaacaa
    22621 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccgg aagaagcgaa
    22681 agagatccca caggttgcag gctctctgga agaagcactg aacgagctgg atctggaccg
    22741 cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
    22801 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
    22861 ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg atttagttgc
    22921 cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt
    22981 cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac
    23041 tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac
    23101 agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc cgcgcaacaa
    23161 ctgctcgccc aaagctcccg caaattgttt ggtacgccgt taccggaact gttgagctac
    23221 ttctcattaa atattgagct gatgcaagaa agtctggagg cggggcaagg ttttaccgat
    23281 aacgaagtga cgctggtcat cgacggtcgc tcgcatatcc tttctgtgac ggcccagcgt
    23341 atgccggacg gcatgatcct gctggagatg gcgccgatgg ataaccagcg ccgcttaagc
    23401 caggaacagc tacaacatgc tcaacaagtt gctgcccgtg atttagtgcg tggcctggca
    23461 catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctact cagcaaagca
    23521 ttacctgacc cgtcactgct ggaatatacc aaagtgatta tcgaacaggc ggaccggctg
    23581 cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt taccgaaagt
    23641 attcacaaag tagctgaacg cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg
    23701 cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt
    23761 gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggaaggcggt
    23821 gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga acgctatcgg
    23881 ctggcggcgc ggattgatgt ggaagataac gggccaggca ttccgcctca tttgcaggat
    23941 acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc
    24001 gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg gccagggcat
    24061 accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga
    24121 tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg
    24181 gagcgggttt aacctgtaca acatttgaga acggcgcgga ggtactggag gcgctggcga
    24241 gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcac
    24301 tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg accgcacatt
    24361 ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gtttgattat ctgcccaaac
    24421 cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat taccaggaac
    24481 agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc
    24541 cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct attagcgtgc
    24601 tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg catcgccaca
    24661 gtccgcgagc caaagcgcca tttatcgctc tgaatatggc ggctatcccg aaggatttga
    24721 tcgaatcaga actgtttggt cacgagaaag gcgcgtttac cggcgcgaat accattcgtc
    24781 aggggcgttt tgaacaggct gatggcggta cattatttct cgatgaaatt ggcgatatgc
    24841 cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg
    24901 gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac cagaatctcg
    24961 aacagcgggt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgttatcc
    25021 gcgttcatct gccgccgctg cgtgagcgtc gggaagatat tccccgcctg gcgcgccatt
    25081 ttttacaggt tgccgcgcga gaactgggcg tagaagcgaa gttgctgcat ccggaaaccg
    25141 aagccgcgtt gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc
    25201 gctggctaac ggtaatggct gccggacagg aagtgttgat tcaggatttg cctggcgaac
    25261 tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagt tgggcgacgc
    25321 ttcttgcgca gtgggcagac agagcgctac gttccggtca tcaaaatctg ctttccgaag
    25381 cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg caggggcata
    25441 aacaggaggc ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt aagttaaaag
    25501 agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag cgcaaattgc
    25561 tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga
    25621 gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca
    25681 caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat gattgggctg tttagttgag
    25741 tttcagattg ctgatgaagt gctctttgtt catgccagat gctgcgtgaa cgccttatcc
    25801 ggcctacaaa agcaatattt tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg
    25861 caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca
    25921 ttcactattt aaatcacccg agagaactgc tgcatccgcg ctttctggcg cagataggta
    25981 tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc
    26041 ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc
    26101 gcaaagtaat cagcgaagtg caaatcccac tgtttctcaa taggggcgta atccagacgg
    26161 aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca
    26221 ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct
    26281 ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc
    26341 agatcggtat caccctgagt ggtgtagccc tggaaattac gatgcagcac gccttcacgc
    26401 tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag
    26461 cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc
    26521 ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcatag
    26581 ttaaagacgc tcagacggtc agggtttagc tccgccacgc gtttcagggt aaaggcgaaa
    26641 ctttccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaagcca
    26701 atctcacgcg catggttaag cagcgcaaag ataaactctt catcctgctc gcggttaacc
    26761 agacgttgca cttctttgtt gaagtcctgc acgcccatac tcaggcgatt aaagccctcg
    26821 gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc
    26881 tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcaggcg gctgatttgc
    26941 gctttattaa gatacgtcgg cgttccgccg ccccagtgca gctggctgac gtgacgcccg
    27001 gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc
    27061 ttgtgctgct ggcgagtaac aatcttattg cagccgcaga agtaacaaag cttatggcag
    27121 aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa
    27181 aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt
    27241 ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac
    27301 atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc
    27361 gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga
    27421 ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg
    27481 cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt
    27541 cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat
    27601 ccagtttagc atccacccag gattgctctt cggcactcag ggtttcgcct gcttccagac
    27661 gttccagcag cgcatccaga cgctcatccg tttctagtaa ttccaactcc gcctgcggtg
    27721 aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca
    27781 atggaatagg ggttttactg ccaatacgtg gatctttcgg tgcgttctgg cctttgctgc
    27841 ctgacgtgtt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct
    27901 gacgcttgcg gtcacgagct tcctgattca gctcttcgcg cgttttgcga cgcgcttttg
    27961 catgaccttt gctgcgtgat ggtgaagatg gtttcataat ggttcgtctt ttagccgttt
    28021 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    28081 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    28141 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    28201 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    28261 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    28321 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    28381 atataaaagc aattaactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    28441 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    28501 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccgc aaagctttga
    28561 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    28621 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    28681 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    28741 acctcaaaaa cccctggacg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    28801 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    28861 tggcagcgcg cgctcggcga gtacctcgaa aaacgtcaga gcctacaagg tctggtagtg
    28921 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    28981 gacagcaata tcgccgtgct ggtactgctg accaaagcgg acaaactggc aagcggcgca
    29041 cgtaaagcgc aattgaatat ggtgcgtgaa gcggtgctgg cgtttaacgg tgatgtgcag
    29101 gttgaaacgt tttcttcgct gaagaaacaa ggcgtggaca agctgcggca gaaactggat
    29161 acctggttta acgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttac
    29221 cttaatgctt atgccggatg tggcgtatcc ggcccataaa ttcaatagat tacaattcta
    29281 ccgttggcct gataaacatg gtgcatcagg cattacggat ctttcctttc gcccaataaa
    29341 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    29401 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    29461 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    29521 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    29581 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    29641 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg caacggcatc
    29701 gacatcatct ctatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    29761 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    29821 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    29881 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    29941 gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    30001 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    30061 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    30121 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    30181 gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaga gatgcgccat
    30241 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    30301 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    30361 atcggttgac gataaacgtc ccgttgcagt tacagcctgg tgataagagg tatgcacacg
    30421 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgatt tcagcttcgc
    30481 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    30541 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taatgccctg
    30601 tttttcaaac agaatggttt gtaactgctt ggtggaagaa aggttaaatt cctcacctgc
    30661 aatttcatgc gctttctttt ccagctcagc cagacgaagg gtgagctctt cagaatgatt
    30721 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gtactggcac
    30781 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    30841 ccacattttc agatgcaact gcaaggtgac atcagcatct tcggcggcgt aacgtccggc
    30901 ctcttcgagg gcaatctggt taaaagtcag ttgatttttg cctttacccg caatctcttc
    30961 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    31021 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagctc
    31081 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    31141 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag agatttgatc
    31201 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    31261 aaaagaaagc ccgaccaggt tagctgagat gttatcaagg ctgtcggttt cggtatcaaa
    31321 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    31381 gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggtg cttcgtctgc
    31441 aacactggtt tcctgtggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    31501 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    31561 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacgtcggt
    31621 tttaatcgtc gccagctggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    31681 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    31741 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    31801 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaaatcga tgatcagttc
    31861 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    31921 gttgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctgat
    31981 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    32041 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcaaggg
    32101 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    32161 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    32221 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    32281 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    32341 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    32401 gattatgggt aaaggatgcc acagacagga taaaaacatg agtttttcac cgcttttcgt
    32461 gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt catgtggata actttgtgca
    32521 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    32581 tgtttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    32641 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcgttt
    32701 tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa ccttttgaat
    32761 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    32821 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    32881 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    32941 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    33001 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    33061 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    33121 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    33181 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtaaa
    33241 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    33301 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttc
    33361 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    33421 caattcgata aactgcttaa tgttacgttg tgttatccgg acaataaccg tcagccgttc
    33481 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    33541 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    33601 tggctgaact cattgtggca ggaaaaagac cgacttctga cgtcgttgat gtcatcacag
    33661 cggcaggaca agtaaatttt gctcgcctct gaatgagcag aggcgagtga gtattttaat
    33721 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    33781 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    33841 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    33901 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    33961 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    34021 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    34081 tccatcttac cggttgttta atggtcggct cactttgttg taataacggt aaatactctt
    34141 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaat tcagggtgtg
    34201 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc accgctgagg
    34261 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    34321 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    34381 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    34441 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    34501 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    34561 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ctatcaccat
    34621 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    34681 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    34741 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactaagttc acttttgccg
    34801 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    34861 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    34921 tatcgaatgt catttgcatt tgaaaacggg aattaaaaaa gcctttgtca aaattttgat
    34981 aacttaacgt catatcgtag gcatgttcac ctttcttttt agcatcgtta aaatctttaa
    35041 taaatttttc gacaccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    35101 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    35161 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc g
//
LOCUS       DAZGXS010000034        34708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGXS010000034 DAZGXS010000000
VERSION     DAZGXS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..653
                     /locus_tag="ACHKG5_001824"
                     /pseudo
     CDS             1..653
                     /locus_tag="ACHKG5_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
     gene            complement(803..2167)
                     /gene="mnmE"
                     /locus_tag="ACHKG5_001825"
     CDS             complement(803..2167)
                     /gene="mnmE"
                     /locus_tag="ACHKG5_001825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0867939.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2273..3919)
                     /gene="yidC"
                     /locus_tag="ACHKG5_001826"
     CDS             complement(2273..3919)
                     /gene="yidC"
                     /locus_tag="ACHKG5_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0867940.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3922..4179)
                     /gene="yidD"
                     /locus_tag="ACHKG5_001827"
     CDS             complement(3922..4179)
                     /gene="yidD"
                     /locus_tag="ACHKG5_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0867941.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4143..4502)
                     /gene="rnpA"
                     /locus_tag="ACHKG5_001828"
     CDS             complement(4143..4502)
                     /gene="rnpA"
                     /locus_tag="ACHKG5_001828"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0867942.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4519..4659)
                     /gene="rpmH"
                     /locus_tag="ACHKG5_001829"
     CDS             complement(4519..4659)
                     /gene="rpmH"
                     /locus_tag="ACHKG5_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0867943.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4889..4969)
                     /locus_tag="ACHKG5_001830"
     CDS             complement(4889..4969)
                     /locus_tag="ACHKG5_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867944.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5266..6669
                     /gene="dnaA"
                     /locus_tag="ACHKG5_001831"
     CDS             5266..6669
                     /gene="dnaA"
                     /locus_tag="ACHKG5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0867945.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6674..7774
                     /gene="dnaN"
                     /locus_tag="ACHKG5_001832"
     CDS             6674..7774
                     /gene="dnaN"
                     /locus_tag="ACHKG5_001832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0867946.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7774..8847
                     /gene="recF"
                     /locus_tag="ACHKG5_001833"
     CDS             7774..8847
                     /gene="recF"
                     /locus_tag="ACHKG5_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0867947.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8876..11290
                     /gene="gyrB"
                     /locus_tag="ACHKG5_001834"
     CDS             8876..11290
                     /gene="gyrB"
                     /locus_tag="ACHKG5_001834"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0867948.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11314..11412)
                     /locus_tag="ACHKG5_001835"
                     /pseudo
     CDS             complement(11314..11412)
                     /locus_tag="ACHKG5_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11530..11928
                     /gene="yidB"
                     /locus_tag="ACHKG5_001836"
     CDS             11530..11928
                     /gene="yidB"
                     /locus_tag="ACHKG5_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0867949.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12043..12855
                     /gene="yidA"
                     /locus_tag="ACHKG5_001837"
     CDS             12043..12855
                     /gene="yidA"
                     /locus_tag="ACHKG5_001837"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0867950.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12901..13557)
                     /gene="yidX"
                     /locus_tag="ACHKG5_001838"
     CDS             complement(12901..13557)
                     /gene="yidX"
                     /locus_tag="ACHKG5_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP0867951.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13693..13806
                     /locus_tag="ACHKG5_001839"
                     /pseudo
     CDS             13693..13806
                     /locus_tag="ACHKG5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13803..14867)
                     /gene="cbrA"
                     /locus_tag="ACHKG5_001840"
     CDS             complement(13803..14867)
                     /gene="cbrA"
                     /locus_tag="ACHKG5_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0867952.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14968..16182
                     /gene="yidR"
                     /locus_tag="ACHKG5_001841"
     CDS             14968..16182
                     /gene="yidR"
                     /locus_tag="ACHKG5_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0867953.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16184..16516)
                     /gene="yidQ"
                     /locus_tag="ACHKG5_001842"
     CDS             complement(16184..16516)
                     /gene="yidQ"
                     /locus_tag="ACHKG5_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0867954.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16756..16828
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16822..17235
                     /gene="ibpA"
                     /locus_tag="ACHKG5_001843"
     CDS             16822..17235
                     /gene="ibpA"
                     /locus_tag="ACHKG5_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0867955.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17347..17775
                     /gene="ibpB"
                     /locus_tag="ACHKG5_001844"
     CDS             17347..17775
                     /gene="ibpB"
                     /locus_tag="ACHKG5_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0867956.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17971..19632
                     /gene="yidE"
                     /locus_tag="ACHKG5_001845"
     CDS             17971..19632
                     /gene="yidE"
                     /locus_tag="ACHKG5_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0867957.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19629..20345)
                     /gene="yidP"
                     /locus_tag="ACHKG5_001846"
     CDS             complement(19629..20345)
                     /gene="yidP"
                     /locus_tag="ACHKG5_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0867958.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20641..22257
                     /locus_tag="ACHKG5_001847"
     CDS             20641..22257
                     /locus_tag="ACHKG5_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0867959.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22257..23579
                     /locus_tag="ACHKG5_001848"
     CDS             22257..23579
                     /locus_tag="ACHKG5_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFP0867960.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23576..24469)
                     /gene="yidL"
                     /locus_tag="ACHKG5_001849"
     CDS             complement(23576..24469)
                     /gene="yidL"
                     /locus_tag="ACHKG5_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0867961.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24636..26351
                     /gene="yidK"
                     /locus_tag="ACHKG5_001850"
     CDS             24636..26351
                     /gene="yidK"
                     /locus_tag="ACHKG5_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0867962.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26348..27841
                     /gene="yidJ"
                     /locus_tag="ACHKG5_001851"
     CDS             26348..27841
                     /gene="yidJ"
                     /locus_tag="ACHKG5_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0867963.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQSRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27888..28337)
                     /gene="yidI"
                     /locus_tag="ACHKG5_001852"
     CDS             complement(27888..28337)
                     /gene="yidI"
                     /locus_tag="ACHKG5_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP0867964.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28446..28793
                     /gene="yidH"
                     /locus_tag="ACHKG5_001853"
     CDS             28446..28793
                     /gene="yidH"
                     /locus_tag="ACHKG5_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0867965.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28783..29145
                     /gene="yidG"
                     /locus_tag="ACHKG5_001854"
     CDS             28783..29145
                     /gene="yidG"
                     /locus_tag="ACHKG5_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0867966.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29142..29639
                     /gene="yidF"
                     /locus_tag="ACHKG5_001855"
     CDS             29142..29639
                     /gene="yidF"
                     /locus_tag="ACHKG5_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0867967.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29647..30831)
                     /gene="emrD"
                     /locus_tag="ACHKG5_001856"
     CDS             complement(29647..30831)
                     /gene="emrD"
                     /locus_tag="ACHKG5_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0867968.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30913..30987
                     /gene="ysdE"
                     /locus_tag="ACHKG5_001857"
     CDS             30913..30987
                     /gene="ysdE"
                     /locus_tag="ACHKG5_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0867969.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31111..31200)
                     /gene="tisB"
                     /locus_tag="ACHKG5_001858"
     CDS             complement(31111..31200)
                     /gene="tisB"
                     /locus_tag="ACHKG5_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP0867970.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31765..31863
                     /gene="ivbL"
                     /locus_tag="ACHKG5_001859"
     CDS             31765..31863
                     /gene="ivbL"
                     /locus_tag="ACHKG5_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0867971.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31969..33657
                     /gene="ilvB"
                     /locus_tag="ACHKG5_001860"
     CDS             31969..33657
                     /gene="ilvB"
                     /locus_tag="ACHKG5_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0867972.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33661..33951
                     /gene="ilvN"
                     /locus_tag="ACHKG5_001861"
     CDS             33661..33951
                     /gene="ilvN"
                     /locus_tag="ACHKG5_001861"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0867973.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34214..>34708
                     /locus_tag="ACHKG5_001862"
     CDS             34214..>34708
                     /locus_tag="ACHKG5_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867974.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMK"
BASE COUNT         8406 a         8751 c         9164 g         8387 t
ORIGIN      
        1 tataaaagcc aaaatgttta aaaatgaatt aaataaagaa gatatattga aactgatggc
       61 agcaagagaa aaagtggcgg gagcatcaga caaatggacg aaggcatcag gcttatattc
      121 tgcgatagtg aaagggcata cgaagattgt tgctgcctgg atggagacag ctgaagtgat
      181 agccagccat tatgaaaatg ataaagatgt agtgagagaa ctcctgtcgc tgagcagaaa
      241 taatgcagtt tgctctttgt atgttgccag ctataagaca atgagtaagc aggtcattga
      301 tgtatatctg aatgcggcga ttcgcctggc gttgcaacac gggttcactt tcgatgagat
      361 tttggagcag tttacccgtg actttgatgg gaagtcattc tctcttgcgg tagagaaagc
      421 ggatgatata tatgggtctc tggctgaaaa tattcaaaat tgtggttggt gaaaatgaaa
      481 attatcttaa agatgtgatg atgcaactgg aagcgaagaa taatgaaggc aaatatgtaa
      541 taagcaaggc aaatggcaat cctgtattta aagaactatt ctggaaagca atagacgaat
      601 ttaactttcc gcaagaggag cttaatcggc taaagcaata tcgttctctg taaagacagg
      661 actgcaagat gttgaaagtc gctccagtca gtagtaaagg cagttttggt gcaggatgct
      721 cataaaaaaa caagcgctgt tccgttctga tagttagttt cccccagaac ggaacagtgc
      781 cctttatatc aatttagacg acttacttac caatacagaa gctggagaaa atccgcccca
      841 gcaaatcatc agaggtaaat tccccggtga tttcgcttaa gttctgctgt gccaggcgca
      901 actcttccgc cagcagttcg cccgcccagg cacccaacag ttgcgcttta ccttgctgca
      961 aatgctccgc tgcctgttcc agcgcctgaa ggtggcgacg acgcgccagg aagccgcctt
     1021 ccatgttggt gtcaaagccc atgctctgtt tgagatggtt acgcagcacg tccacgcctt
     1081 cgccagtctt tgccgagaga cgaatcaacg catgaccgtt cacttcactc attcccagcg
     1141 tttcgccagt gatatcggct ttattgcgta ccacggtgat cggcagtttc gcgggcagac
     1201 gggcgataaa ttccggccag atctccgccg gatcaacggc gtctgtcgtg gtgccatcga
     1261 ccataaacag cacgcggtcg gcttgttcaa tttcttgcca cgcgcgctcg ataccaatac
     1321 gttccacttc atcgctggct tcacgtagcc cggcggtgtc gatgatatgc agcggcattc
     1381 cgtcaatgtg gatatgctca cgcaacacgt cacgcgtggt tccggcgata tcggttacga
     1441 ttgctgcttc acggccagcc agcgcgttta acaggctcga tttaccggca ttaggacgtc
     1501 cggcaatcac caccttcatc ccttcgcgca acaaactacc ctgacgtgct tcagcacgca
     1561 ctgcatcgag atcggcaatc acgtcattaa gctgggcttc aatttttccg tcggagagga
     1621 aatcgatctc ttcatcgggg aaatcaattg ccgcttcgac gtaaatgcgc aagtgggtga
     1681 gggcttccac cagatggttg acccgtgcgg agaatgcgcc ttgcagcgag ttaagtgccg
     1741 aacgggctgc ctgttccgaa ctggcatcga taagatcggc aatcgcctcg gcctgggcta
     1801 agtcgagttt atcgttaaga aacgcgcgtt cggaaaactc accagggcga gcaatccgca
     1861 ggccgggaat ggtcagaatg cgttttaaca gcaggtcgag aatcaccgga ccgccatgac
     1921 cctgcagttc cagcacatct tcgccggtga acgagttcgg gccagggaac catagcgcaa
     1981 tcccctgatc gagcacgctg ccatcggcgt ctttaaacgg aagataatcg gcgtagcgcg
     2041 gcttaggcag tttacccagc acggtttcgg caacttcacg ggctttgaag ccggagatgc
     2101 gcaggatgcc aacgccgcca cgtcccggag gcgtggcctg ggctacgata gtgtcattat
     2161 cgctcatgat gttcctgttg ctttgtgtgg cggatacgcg gtgcttatcc gccctacgaa
     2221 aagaaaaaag gcggtcaact gaccgccctt attttagcga aaactcaccg aatcaggatt
     2281 ttttcttctc gcggctatgc aggccacgtt tttccagacc acggtaaatc agctgctgct
     2341 gaataatggt taccaggttg ctgacgatat agtacagcac cagacctgac gggaaccaca
     2401 ggaagaacac ggtgaagatg accggcataa aggtcatgat cttctgctgc atcgggtcgg
     2461 tcacagtggt cggcgacatc ttctgaatga agaacatcgt tacgcccatc aggatcggca
     2521 ggatgtagta cgggtcctgt gcagacaggt cgtggatcca cagtgcaaac ggtgcctgac
     2581 gcagttcaac ggaacccatc agcatgtagt acaacgccag gaagattggc atctggatca
     2641 gcagcgggaa gcagccgccc agcgggttaa ccttctcagc tttgtacagc gccatcattt
     2701 cctggctgat acgctgtttg tcatcgccca gacgctcacg cattgcctga atcttcggct
     2761 gcagcatacg catcttcgcc atggaggtgt actgcgcttt ggtcagcggg tacatgatgc
     2821 cacgaacgat aaaggtgatg atgataatgg agaagcccca gttacccaca aagctatgga
     2881 tccatttcag cagtttgaac agcggctgag agatgaacca caaccaaccg taatcaacgg
     2941 tcagatccag gtgcggagca acagctgcca ttttgtcctg gatttccggg ccaacccaca
     3001 gggtgctgtt catcgcgcca gtctgaccag gctgaaccag taccggctga gatttatagc
     3061 cgatagcggc gatgccgtta cccagattag cagtatagaa gttgttggta ccgtcgttat
     3121 gcgggatcca cgccgtcgcg aaatactgtt gcagcattgc cacccaacca cctttcgaag
     3181 agatgttcag gttttcgtta tcggcaatgg tatcgaactt gtatttctcg tacttctcgt
     3241 caggcgtgga gtacgccgcg ccacggaagg tgtgcagtgc gaagttgctg cttccggtat
     3301 cgagatgcgg tggcagagtg atggattgct tcaactgacc aaaggtggag atttccagcg
     3361 gtttctcgcc agcgttctgc acgttgtagt tgacgttgac agcgtaatca ccccgtttca
     3421 ggacaaacgt tttggtaaac gtgttgcctg ccgcgtcggt atacgtcatc ggcacctgca
     3481 gttcgttttg accttcagcc agcacataag cgtctttttc aacgttatac agcggacgcg
     3541 ggccgttagc cgggttatct gggccatcac gaccggtcag accgctctgt gcctgataaa
     3601 taaactgcgg tgaagtttcc agcagctgga acggctgggt agagttcagc tctttcgggt
     3661 aagcaggcag cagagcttgc tcaacatcac caccacgggt gttgatggtc agatcaagca
     3721 cgtcggtctt aaccgagatc agtttcccct ggccactggc cggtacgccc tggtcggcgg
     3781 cgctacccgc tgcggtggtc gttgtctgcg tggtctgttg ggcctgaggt tgcgggtttt
     3841 tatcctgctc ccaggcttgc cagatcatga aagacacgaa cagcaaagcg atgactaaaa
     3901 gattgcgttg cgaatccatc gttagtgttc tctggtatta aatggtccgg gcgggacggg
     3961 atcgtcacca ccagggtgta aagggtggca ttttaatacg cgtttcaccg tcaaccaact
     4021 gccttttatc actccaaacc tgcgcaatgc ctcaattccg tagcttgaac aggttggagt
     4081 gaaacgacaa tgcggcccga gtagcggact aatcaggcgt tgatagaccc gaatgagggc
     4141 tatcaggacc cgcgagccag gcgacagtgg cggcgccata atttttccaa cgcttccgag
     4201 agagcacggt tatcgaggtt ggcaacccct tttttcgcca ccaccacgaa atccatagcc
     4261 gggagttcat gttggcgcag acggaagctt tcacgcgtca gacgtttaat ccgattgcgt
     4321 tcatgggcgc gtcgaacgtt tttcttggcg actgtaagac cgatacgggg atgccccagc
     4381 gaattcaggc ggccgagaat ggtaatttgc ggcgtgccag cccgttgtgg ctgctggaag
     4441 acgaatgtga attgactggg agttaacaag cgtaactccc tgggaaatgc gagcttaacc
     4501 actcaggggt tagctttatt acttagaaac ggtcagacga gcgcggcctt tagcacgacg
     4561 acgtgccaga acctgacgac catttttagt agccatacga gcacggaagc cgtgagaacg
     4621 gttgcgcttc agtacagacg gttgaaaagt gcgtttcatg gcgatttcta cctaaacttg
     4681 aataaattca atggctttat tggatatccg ccgaaaaatg aaacgatgga caccgaagcc
     4741 atgggtgatt aaagaggccg gattgtaata attgtacact ccggagtcaa ttctctttcc
     4801 ttatttaccg cgcttttccg caccttttcg cagggaaaac gtacggcctc acaccagtga
     4861 aaaccagcat ggcgcgccgg gtggaggatt atacgggctg atgggtaaag cgcaaggatc
     4921 gtcctggatc tttattagat cgattaagcc aatttttgtc tatggtcatt aaattttcca
     4981 atatgcggcg taaatcgtgc ccgcctcgcg gcaggatcgt ttacacttag cgagttctgg
     5041 atcatcctgt ggataaatcg ggaaaatctg tgagaaacag aagatctctt gcgcagttta
     5101 ggctatgatc cgcggtcccg atcgttttgc aggatcttga tcgggcatat aaccgcagac
     5161 agcggttcgt gcgtcaccct caagcagggt cttttcgacg tacgtcaaca atcatgaatg
     5221 tttcagcctt agtcattatc gacttttgtt cgagtggagt ccgccgtgtc actttcgctt
     5281 tggcagcagt gtcttgcccg attgcaggat gagttaccag ccacagaatt cagtatgtgg
     5341 atacgcccat tgcaggcgga actgagcgat aacacgctgg ccctgtacgc gccaaaccgt
     5401 tttgtcctcg attgggtacg ggacaagtac cttaataata tcaatggact gctaaccagt
     5461 ttctgcggag cggatgcccc acagctgcgt tttgaagtcg gcaccaaatc ggtgacgcaa
     5521 acgccacaag cggcagtgac gagcaacgtc gcggcccctg cacaggtggc gcaaacgcag
     5581 ccgcaacgtg ctgcgccttc tacgcgctca ggttgggata acgtcccggc cccggcagaa
     5641 ccgacctatc gttctaacgt aaacgtcaaa cacacgtttg ataacttcgt tgaaggtaaa
     5701 tctaaccaac tggcgcgcgc ggcggctcgc caggtggcag ataaccctgg cggtgcctat
     5761 aacccgttgt tcctttatgg cggcacgggt ctgggtaaaa ctcacctgct gcatgcggtg
     5821 ggtaacggca ttatggcgcg caagccgaat gccaaagtgg tttatatgca ctccgagcgc
     5881 tttgttcagg acatggttaa agccctgcaa aacaacgcga tcgaagagtt taaacgctac
     5941 taccgttccg tagatgcact gctgatcgac gatattcagt tttttgctaa taaagaacga
     6001 tctcaggaag agtttttcca caccttcaac gccctgctgg aaggtaatca acagatcatt
     6061 ctcacctcgg atcgctatcc gaaagagatc aacggcgttg aggatcgttt gaaatcccgc
     6121 ttcggctggg ggctgactgt ggcgatcgaa ccgccagagt tagaaacccg cgtggcgatc
     6181 ctgatgaaaa aggccgacga aaatgacatt cgtttgccag gcgaagtggc gttctttatc
     6241 gccaagcgtc tacgatctaa cgtacgtgag ctggaaggcg cgctgaaccg cgtcattgcc
     6301 aacgccaact ttaccggacg ggcgatcact atcgacttcg tgcgtgaggc gctgcgcgac
     6361 ttgctggcat tgcaggaaaa actggtcacc atcgacaata ttcagaagac ggtggcggag
     6421 tactacaaga tcaaagtcgc ggatctcctt tccaagcgtc gatcccgctc ggtggctcgt
     6481 ccgcgtcaga tggcgatggc gctggcgaaa gagctgacta accacagtct gccagagatt
     6541 ggcgatgcgt ttggtggtcg tgaccacact acggtgcttc atgcctgccg gaagatcgag
     6601 cagctgcgtg aagagagcca cgatatcaaa gaagattttt caaatttaat cagaacattg
     6661 tcatcgtaaa cctatgaaat ttaccgtaga acgtgagcat ttattaaaac cgctacaaca
     6721 ggtgagcggt ccgttaggtg gtcgtcctac gctaccgatt ctcggtaatc tgctgttaca
     6781 ggttgctgac ggtacgttgt cgctgaccgg tactgatctc gagatggaaa tggtggcacg
     6841 cgttgcgctg gttcagccac acgagccagg agcgacgacc gttccggcgc gcaaattctt
     6901 tgatatctgc cgtggtctgc ctgaaggcgc ggaaattgcc gtgcagctgg aaggtgagcg
     6961 gatgctggtg cgctccgggc gtagccgttt ttcgctgtct accctgccag cggcggattt
     7021 cccgaacctc gatgactggc agagtgaagt cgaatttacc ctgccgcagg cgacgatgaa
     7081 gcgtctgatt gaagcgaccc agttttctat ggcgcatcag gacgttcgct attacttaaa
     7141 cggtatgctg tttgaaaccg aaggtgaaga actgcgcacc gtagcgaccg acggccaccg
     7201 tctggcggtc tgttcaatgc caattggtca atctttgcca agccattcgg tgatcgtgcc
     7261 gcgtaaaggc gtgattgaac tgatgcgtat gctcgacggc ggcgataatc cgctgcgtgt
     7321 gcagattggc agcaacaata ttcgcgccca cgttggcgac tttatcttca cctctaaact
     7381 ggtggatggt cgcttcccgg attaccgccg cgttctgccg aagaatccgg acaaacatct
     7441 ggaagcaggc tgcgatctcc tcaagcaggc gtttgctcgc gcggcaattc tctctaacga
     7501 gaaattccgc ggtgtgcgcc tgtatgtcag cgaaaaccag ctgaaaatca ccgccaacaa
     7561 cccggaacag gaagaagcgg aagagatcct cgacgttacc tatagcggtg cggagatgga
     7621 aatcggcttc aacgtcagct atgtgctgga tgttctgaac gcgctgaaat gcgaaaacgt
     7681 ccgcatgatg ctgaccgatt cggtttccag cgtgcagatt gaagatgcag ccagccagag
     7741 cgcggcttat gttgtcatgc caatgagact gtaatgtccc tcacccgctt gttgatccgc
     7801 gatttccgca acattgaaac cgcggatctc gctttatctc ccggctttaa ctttctggta
     7861 ggtgccaacg gcagtggcaa aaccagcgtg ctggaagcca tctatacgct cggccatggt
     7921 cgggcgtttc gcagtttgca gattggtcgc gtcattcgcc acgagcagga ggcgtttgtt
     7981 ctccacgggc gattacaggg cgaagagcgc gagacggcga ttggcttaac caaagacaaa
     8041 cagggcgaca gcaaagtccg catcgacggt actgacgggc ataaagtcgc ggaactggcg
     8101 cacctgatgc caatgcagct gataacgcca gaagggttta ctttactcaa cggcggcccc
     8161 aaatacagaa gagcattcct cgactgggga tgctttcaca acgaacccgg atttttcacc
     8221 gcctggagca atctcaagcg attgctcaag cagcgcaatg cggcgctgcg ccaggtgaca
     8281 cgttacgaac agctacgccc gtgggataaa gaactgatcc cgctggcgga gcaaatcagt
     8341 acctggcgcg cggagtatag cgccggtatc gcggccgata tggctgatac ctgtaagcaa
     8401 tttctccctg agttttctct gactttctct ttccagcgcg gctgggagaa agagacagaa
     8461 tatgctgagg tgctggaacg taattttgaa cgcgatcgcc agctaaccta caccgcgcat
     8521 ggcccgcata aagcggactt acgcattcgc gccgacggtg cgccggtgga agatacctta
     8581 tcgcgtgggc agcttaagct gttgatgtgc gccttacgtc tggcgcaagg agagttcctc
     8641 acccgtgaaa gcgggcggcg gtgtctctac ctgatagatg attttgcctc tgagcttgat
     8701 gatgagcgtc gcgggctgct tgccagccgc ttaaaagcta cgcaatcgca ggtctttgtc
     8761 agcgcgatca gtgctgaaca cgttatagac atgtcggacg aaaattcgaa gatgtttacc
     8821 gtggaaaagg gtaaaataac ggattaaccc aagtataaat gagcgagaaa cgttgatgtc
     8881 gaattcttat gactcctcca gtatcaaagt cctgaaaggg ctggatgcgg tgcgtaagcg
     8941 cccgggtatg tatatcggcg acacggatga cggcaccggt ctgcaccaca tggtattcga
     9001 ggtggtagat aacgctatcg acgaagcgct cgcgggtcac tgtaaagaaa ttatcgtcac
     9061 cattcacgcc gataactctg tctctgtaca ggatgacggg cgcggcattc cgaccggtat
     9121 tcacccggaa gagggcgtat cggcggcgga agtgatcatg accgttctgc acgcaggcgg
     9181 taaatttgac gataactcct ataaagtgtc cggcggtctg cacggcgttg gtgtttcggt
     9241 agtaaacgcc ctgtcgcaaa aactggagct ggttattcag cgcgagggta aaattcaccg
     9301 tcagatctac gaacacggtg taccgcaggc accgctggcg gttaccggcg agactgaaaa
     9361 aaccggcacc atggtgcgtt tctggcccag cctcgaaacc ttcaccaatg tgaccgagtt
     9421 cgaatatgaa attctggcga aacgtctgcg tgagttgtcg ttcctcaact ccggcgtttc
     9481 cattcgtctg cgcgacaagc gtgacggcaa agaagaccac ttccactatg aaggcggcat
     9541 caaagcgttc gttgaatatc tgaacaagaa caaaacgccg atccacccaa atatcttcta
     9601 cttctccacc gaaaaagacg gtatcggcgt tgaagtggcg ctgcagtgga acgatggctt
     9661 ccaggaaaac atctactgct ttaccaacaa cattccgcag cgtgacggcg gtactcacct
     9721 ggcaggcttc cgtgcggcga tgacccgtac cctgaatgcc tacatggaca aagaaggcta
     9781 cagcaaaaaa gccaaagtca gcgccaccgg tgacgatgcg cgtgaaggcc tgattgcggt
     9841 cgtttccgta aaagtgccgg acccgaaatt ctcctcccag accaaagaca aactggtttc
     9901 ttctgaggtg aaatcggcgg ttgaacagca gatgaacgaa ctgttggcgg aatacctgct
     9961 ggaaaaccca actgacgcga aaatcgtggt tggcaaaatt atcgatgctg cccgtgcccg
    10021 tgaagcggcg cgtcgcgcgc gtgaaatgac ccgccgtaaa ggtgcgctcg acttagcggg
    10081 cctgccgggc aaactggcag actgccagga acgcgatccg gcgctttccg aactgtacct
    10141 tgtggaaggg gactccgcgg gcggctctgc gaagcagggg cgtaaccgca agaaccaggc
    10201 gattctgccg ctgaagggta aaatcctcaa cgtcgagaaa gcgcgcttcg ataagatgct
    10261 ctcttctcag gaagtggcga cgcttatcac cgcgcttggt tgtggtatcg gtcgtgacga
    10321 gtacaacccg gacaaactgc gttatcacag catcatcatc atgaccgatg cggacgtcga
    10381 cggctcgcac attcgtacgc tgctgttgac cttcttctat cgtcagatgc cggaaattgt
    10441 tgaacgcggt cacgtctaca tcgctcagcc gccgctgtac aaagtgaaga aaggcaagca
    10501 ggaacagtac attaaagacg acgaagcgat ggatcagtac cagatctcta tcgcgctgga
    10561 cggcgcaacg ctgcacacca acgccagcgc accggcattg gctggcgaag cgttagagaa
    10621 actggtgtct gagtacaacg cgacgcagaa aatgatcaac cgcatggagc gtcgttatcc
    10681 gaaagcaatg ctgaaagagc ttatctatca gccgacgctg acggaagccg acctctctga
    10741 tgagcagacc gttacccggt gggtgaacgc gctggtcagc gaactgaacg acaaagaaca
    10801 gcacggcagc cagtggaagt ttgatgtcca caccaatgcc gaacaaaacc tgttcgagcc
    10861 gattgttcgc gtgcgtaccc acggtgtgga tactgactat ccgctggatc acgagtttat
    10921 cactggtggc gaatatcgtc gtatctgcac gctgggtgag aaactgcgtg gcttgctgga
    10981 agaagatgcg tttatcgaac gtggcgagcg tcgtcagccg gtagccagct tcgagcaggc
    11041 gctggactgg ctggtgaaag agtcccgtcg cggcctctcc atccagcgtt ataaaggtct
    11101 gggcgagatg aacccggaac agctgtggga aaccaccatg gacccggaaa gccgtcgtat
    11161 gctgcgcgtt accgttaagg atgcgattgc cgctgaccag ttgttcacca cgctgatggg
    11221 cgacgccgtt gaaccgcgcc gtgcgtttat tgaagagaac gccctgaaag cggcgaatat
    11281 tgatatttaa tggcgttaac aatgcgagcg tgcctgatgc gctacgctta tcaggcctac
    11341 gagactcctg caatatattg aatttgcatg tttttgtagg ccggataagg cgttcacgcc
    11401 gcatccggca agaagctata agaaaagggc ggagatcatc tccgcccttt ttatttctgc
    11461 aatccgcaat tatcatcccg cagttctata ctttttgggt caacacgtaa cgctttacag
    11521 gtgattatca tggggctttt tgatgaagtt gtcggcgcct ttctgaaagg cgatgcgggg
    11581 aaatatcagg cgattttaag ttgggttgag gagcagggtg gcattcaggt gctgctggaa
    11641 aaactgcaaa gtggcggctt aggggccatt ctctcaacct ggctgagtaa tcaacagggc
    11701 aatcaaccag ttagcggcga gcaactggaa tcagcactcg gcacaaacgc ggtatccgat
    11761 cttggacaaa aacttggcgt ggataccagt acggcttcct gtttactggc agaacaattg
    11821 ccgaagatta ttgatgcgct ctcaccgcaa ggtgaagtgt caccacaagc caataacgat
    11881 ctgctttccg caggcatgga actgctgaaa gggaaactct tccgctaagc aaatggggag
    11941 catgcaaccc gcgttgcatc ccccttttcc ctcgggtgtt gtactgattt ttgagcggaa
    12001 tcgcgttagc atgggtcagg aaccaatcta cctggggaac tcatggctat caaactcatt
    12061 gctatcgata tggatggcac ccttctgctg cccgatcaca ccatttcacc tgccgttaaa
    12121 aatgcgattg ccgcagctcg cgcccgtggc gtgaatgtcg tgctaacgac gggtcgccct
    12181 tatgcaggtg ttcacaatta cctgaaagag ctgcatatgg aacagccggg cgactactgc
    12241 attacttata acggcgcgct ggtacagaag gccgctgatg gtaacaccgt ggcgcaaact
    12301 gctctcagct atgacgacta tcgtttcctg gaaaaactct ctcgcgaagt cggttctcat
    12361 ttccacgccc tggaccgcac cacgctgtac accgccaacc gtgatatcag ctactacacg
    12421 gtgcatgaat ccttcgttgc caccattccg ctggtgttct gcgaagcgga gaaaatggac
    12481 cccaataccc agttcctgaa agtgatgatg attgatgaac ccgccatcct cgaccaggct
    12541 atcgcgcgta ttccgcagga agtgaaagag aaatataccg tgctgaaaag tgcgccgtac
    12601 ttcctcgaaa tccttgataa acgcgttaac aaaggtacgg gtgtgaaatc actggccgac
    12661 gtgttaggta ttaaaccgga agaaatcatg gcgattggcg atcaggaaaa cgacatcgca
    12721 atgattgaat atgcaggcgt cggtgtggca atggataatg ctattccttc ggtgaaagaa
    12781 gtggcgaact ttgtcaccaa atccaacctt gaagatggcg tggcgtttgc tattgagaag
    12841 tatgtgctga attaatctgt gggcgggcaa acagcataaa cgcgtttgcc cgcttactga
    12901 ttattggttt actgcatata caaccagaaa ttgcagcggc gcagtaatga tgtccagcgt
    12961 aacagtcact ggcagtaatg ctgcggaaac accatccgga atgcctctgg gtgaatagta
    13021 ttcataaaat cgcactttaa atggatggta gaacgccatc acctttgagt aatccatgtt
    13081 tttatttttc tcatggattg tgccttttag gttttccagt gacctggtac agtgttgaac
    13141 accacaggca aaaccataat gcgttgccag tgccttttct tcttcgttat tccagtagta
    13201 gtaaagcgat attgtgccgg taaatttctt tttccccgca tttaaaacaa atcttgcgtc
    13261 attggcaacc tgaatattgt gccggttaag tgctggatca ttgagcatct taacgatgtc
    13321 atcggcacca tcagtgataa ggtaatccag cgattcccct acaaaaacat agccttttgt
    13381 tccattacta tcttttgcct gcgatagccc agtgatggtg tctgactgcg attgatactt
    13441 tcctgaacta ccctttgcgg tatgggaaac cagagcatac gagatacagc ctgaaagcat
    13501 cagcgccagt gggaataaac tggcagcctt aaaaaatccc ttaaaattca acttcatgat
    13561 taatccttta aacgagtagc taagccgttg aaagcatact cttctttccc tcctgtttag
    13621 cgcctgatct cccttttcag atttctgccc gatataagac cttttcttat gaggtgtttt
    13681 tttgtggact aatatctgtt gcaaggctat gggttcgcac ctgcactggt ttatatctcc
    13741 gccgtcgcgt tgattggcgc gctctcttac atcctgctgg tgggcgatgt gacgcgcgtt
    13801 ggctaatctt tcaactgtgg aatatgcgcc ataccactgc gcataatcca ctttcttaaa
    13861 gccggtgcgg tcaggcatcg gctttttact atcttgccga agagttttaa acgcagtttg
    13921 cgggtggcgt gccagtaggc tgcgttgatc ttctctggct gcttcagtaa caccgaacgc
    13981 agaatctctg cgctatccag cgcatagctt atcccctcca gcgagctggc gctgataaat
    14041 cccgccgctt caccaatcag aaaggcgttg tccttaccgc agacaaaatc ctgccagcgt
    14101 gagggaaata gcaccgtgca tttttcgctt tttaccgcct taccaaactg gaattgaaag
    14161 gcgctcattt tctctttcag cgtcgtgaaa cgcgtctgac cgtctttcat tggataggca
    14221 ccgccaaaga taaaataacc gtctttgctg atactccatg agtaacagtc agttatcgca
    14281 ttatcaaaga tgcatgagta gaacggcacc ggatgtttct ctgcgaacca ctgctggata
    14341 gcgacatatt tacgaatctg atggtccggg tagagatatc gccgcaccat cgagttggca
    14401 ccatctgcac cgaccagata gcgggcagta atatgctgct cccaaccgtc tgcacgaaaa
    14461 atgacatgcc atttatcatc ctcacgccag attttccggc acaggctatc gtgataaacc
    14521 tcaacgctgg cggggatcag cgatttcatc cacaagtcga aagcatggcg attaatattg
    14581 atatagcttc gctggtagtt acgcgtgagc gatgcggcga cgtcgacagt tttgacgctg
    14641 aaaatctgtg gattggcgat cacatcgaca ggaagcgtca gtccatcgcg aataaaagat
    14701 cgctgcgcgt ccggtgccag cagaccgcca caaggcttgc tgaaaccttc agtaccatgc
    14761 tggtgctttt tatccagcgc gatcacctgc attttgccgg ctaactttcg tgccaacgcc
    14821 gaccctgccg ggccgaggcc aataatcgcc acgtcgaaat gttccatctt ttcactcaca
    14881 ttcatcacga tggcaaaaga ctattgtctg aaagtgaagt ccttgtgaag tatgatggcg
    14941 ggaaccgttt tgtatcagga ccgcgccatg aaacagataa cctttgctcc ccgtaatcac
    15001 ctgctcacca ataccaatac ctggacgccc gacagccagt ggctggtatt tgacgtgcgt
    15061 ccttctggcg cgtcgtttac cggcgagacc attgagcgtg tgaatatcca taccggcgag
    15121 gtcgaggtta tctatcgcgt gtcacagggc gcgcacgtcg gcgtggtgac cgttcatcca
    15181 aagtcagaga aatatgtctt tatccacgga cctgaaaatc ctcatgaaac atggcattac
    15241 gatttccatc accgtcgcgg cgtgattgtt gaaggcggca agatgaacaa tctcgatgca
    15301 atggatatta ccgttccgta cacatcaggg gcgctgcgtg gcggcagcca tgtgcatgtc
    15361 tttagcccga acggtgaaat ggtgagtttt acctataacg accatgtaat gcatcagttc
    15421 gattcggcgc tggatttgcg aaacgtcggg gttgctgcac cgtttggccc ggtcaacgta
    15481 caaaagcagc atccgcgtga atacagcggt agccactggt gcgtgctggt gagtaaaacc
    15541 acgcccacgc cgcagcctgg cagcgatgaa atcaatcgtg cttatgaaga aggatgggta
    15601 ggaaatcacg cgctggcgtt tattggcgac acactttcgc caaagggcga gaaagtgccg
    15661 gagctgttta tcgttgagtt accgcaagat gaagctggct ggaaagcagc aggtgatgcg
    15721 ccgttaagtg gaacggaaac aaccctgccc gcgccaccgc gtggcatcgt gcagcgacgt
    15781 ttaaccttta cccaccatcg ggcttatccg gggttagtca acgtcccgcg ccactgggtg
    15841 cgctgtaatc cgcagggtac gcaaatcgcg tttttaatgc gagatgataa cggcattgtg
    15901 caactgtggc ttatctcgcc acagggcggc gagccgcgcc agttaaccca taacaaaacg
    15961 gatattcagt ctgcatttaa ctggcatccg tcaggagaat ggttgggctt tgtgctgggt
    16021 aatcgaattg cttgtgccca tgcgcaaagc ggcgaggttg agtatttaac cgaaaaccac
    16081 gccaatccac cttctgcgga cgccgtggtc ttctcgccgg atggtcagtg gctggcgtgg
    16141 atggaaggcg gccagctgtg gatcaccgaa actgatcgct aaatcagtta tcaggcattc
    16201 tggccggtgg aataaccgca ttggtggctt gcgcgttggc ttcgcttttt tcaacgcgtg
    16261 agcgcaccga gctgtctttg cggaacacat cccacggtaa taaaagcgtg tccatcacag
    16321 cggtaaacgg catatcgaga atcgccagcg acttagtgcc ccagttggtt tcatcatcgc
    16381 caatcatggt ggcgctggcg cgggtgccgg gatacgttcc ttctttccct ccggtatggg
    16441 acatcacgct ggagcatcct ccgagtaatg ccattccgct gcatatcatg aacgctaaca
    16501 acacatttct tatcataata aattcatctg ttgatcgtgg gtgttggcct gatgagttat
    16561 agcgatccct tgctgaaaat aacatcatca ttacgtcgct ctgtggcggc tatcgcactt
    16621 taacgtttcg tgctgccccc tcagtctatg caatagacca taaactgcaa aaaaaagtcc
    16681 gctgataagg cttgaaaagt tcatttccag acccattttt acatcgtagc cgatgaggac
    16741 gcgcctgatg ggtgttctgg ctacctgacc tgtccattgt ggaaggtctt acattctcgc
    16801 tgatttcagg agctattgat tatgcgtaac tttgatttat ccccgcttta ccgttctgct
    16861 attggatttg accgtttgtt taaccactta gaaaacaacc agagccagag taatggcggc
    16921 taccctccgt ataacgttga actggtagac gaaaaccatt accgcattgc tatcgctgtg
    16981 gctggttttg ctgagagcga actggaaatt accgcccagg ataatctgct ggtggtgaaa
    17041 ggtgctcacg ccgacgaaca aaaagagcgc acctatctgt accagggcat cgctgaacgc
    17101 aactttgaac gcaaattcca gttagctgag aacattcatg ttcgtggtgc taacctagta
    17161 aatggtttgc tgtatatcga tctcgaacgc gtgattccgg aagcgaaaaa accgcgccgt
    17221 atcgaaatca actaatttcc taaggccgcc ttgcgcggcc tgacatctcc atgctcgccg
    17281 tcagggagca tatgcgaatc ttcggatttg caggtactta ctcgcttctt agaaggagaa
    17341 atgactatgc gtaacttcga tttatcccca ctgatgcgtc aatggatcgg ttttgacaaa
    17401 ctggccaacg cactgcaaaa cgccggtgaa agtcagagct tcccgccgta caacattgag
    17461 aaaagcgacg ataaccacta ccgcattacc cttgcgctgg caggtttccg tcaggaagat
    17521 ttagagattc aactggaagg tacgcgcctg agcgtaaaag gcacgccgga gcagccaaaa
    17581 gaagagaaaa aatggctgca tcaagggctt atgaatcagc catttagcct gagctttacg
    17641 ctggctgaaa atatggaagt ctctggcgca accttcgtaa acggtttact gcatattgat
    17701 ttaattcgta atgagcctga acccatcgca gcgcagcgta tcgctatcag cgaacgtccc
    17761 gcgttaaata gctaactagc tattctcttt gccccgccat tctggtgggg ctttttttgt
    17821 ctttcttctt caacaatgcg ttaaatttcg actgtttaag atatttcggc acgttctttc
    17881 ccgtgataat gtgcgctata aggcaaatct gcttcaccga ttcagccata atccgtagca
    17941 attaaaatgt tatctcaaga gaaatggacg atgagtgata tagcattaac ggtcagtatt
    18001 ttggctttgg tggcagtcgt cggtttgttt atcggcaacg tcaaatttcg cggcatagga
    18061 ttaggtattg gcggcgtgct gtttggtggg atcatcgtcg gccattttgt ttctcaggcg
    18121 gggatgacat taagtagcga tatgctgcat gttattcagg aatttggcct gatcctgttc
    18181 gtttatacca tcgggattca ggtagggccg ggcttctttg cctcattgcg cgtctccgga
    18241 ttacgcctca acctgtttgc tgttctgatc gtcatcatcg gtggtctggt taccgccatc
    18301 ctgcataaac tgtttgatat tccactgccg gtagtgctgg ggattttctc cggtgcggta
    18361 accaatacgc cagcgctggg ggcagggcag cagattttgc gcgacctggg tacaccaatg
    18421 gaaatggtcg atcagatggg gatgagttac gcgatggcgt atccattcgg catttgcggg
    18481 atattgttca ccatgtggat gttgcgggtt attttccgcg tcaatgtcga gacagaagcc
    18541 cagcagcacg agtcttcacg caccaatggc ggcgcgctga tcaagactat caatattcgc
    18601 gttgagaacc ctaacctgca tgatttagcc attaaagatg taccgattct caacggcgac
    18661 aaaattatct gctcgcgtct gaaacgcgaa gaaaccctaa aagttccttc gccagatacc
    18721 attatccaac tgggcgattt gctgcatctg gtgggtcagc cagcggattt acataatgcg
    18781 caactggtga ttggtcagga ggtcgatact tcgctgtcca cgaaaggcac tgatttgcgc
    18841 gtcgagcgtg tggtggtcac caatgaaaac gtgcttggta agcgtattcg cgacctgcat
    18901 tttaaagaac gctatgacgt tgttatctcg cgcctgaacc gtgccggggt cgaactggtc
    18961 gccagtggcg atatcagcct gcagttcggc gatatcctca acctggtggg gcgtccgtcc
    19021 gcaattgatg ccgttgccaa tgtgctgggg aatgcgcagc aaaaactgca acaggttcag
    19081 atgctgccag tgtttattgg catcgggctt ggcgtattgt taggttctat tcccgtcttt
    19141 gtgccaggat tcccggccgc gttgaaactg gggctggcgg gcggtccgct gattatggcg
    19201 ttgatcctcg ggcgtatcgg cagtatcggc aagctgtact ggtttatgcc gccaagtgcc
    19261 aacctcgcgc tgcgggaact ggggatcgtg ctgttcctct cggtcgttgg tctgaaatct
    19321 ggtggggatt ttgtgaatac cctggtcaat ggcgaagggc taagctggat tggttatggt
    19381 gccctgatca ccgccgttcc gctgattact gttggcattc tggcgcggat gttagccaaa
    19441 atgaattacc tgaccatgtg cgggatgctg gcaggttcca tgaccgatcc tccggcgctg
    19501 gcgtttgcta ataatcttca tccaaccagc ggtgcggcgg cgctctctta cgccactgtc
    19561 tatccgttag tgatgttcct gcgcattatc accccccaat tactggcggt gctcttctgg
    19621 agtatcggtt aactcttgtc cgggtggagt cgccgtaaat ggtactccac ctggtattcg
    19681 ctggcatttc tgaacatcac tgaataattc aaaaactccc cgctctcgct atatgacaac
    19741 gaggtgatcc gcagcagtgg cgtgtgttcc gctaccttca tttgccgcgc cagcagtcta
    19801 tcggcgagca ccggcgtcag gctttcataa ttaccgccaa tcaaaatccc gcactcttgt
    19861 tcaatatatt caaacttcga cccttccaga tgttgcagcg aaaggttacg gaacagtttt
    19921 accggcatat agctgtcttc cagcatcagc ggtttccctt ccacaaaacg aacgcgacgg
    19981 gagaagtaga tctgctcgtt gatttgaatc cgcaactggc tggcaattgc cggaggcgca
    20041 ggcatgattt caaaaatcag cacctggctg gtgactgttt ttccctgccg ttttaacacc
    20101 tccaccagcc cggtcaggct ggcggtttga tgcagcacat ctttgcgcac caggtaagtg
    20161 ccgctgccgt ggcggcggac caccagcccc cacgctacca gcaggtcaat cgctttacgg
    20221 atggtcatcc gcgataccgc aaactcttcc gccagctttt tttcaccggg aagaaggctg
    20281 tttagcgtga aatccgcgga attaagtcga attcttaacc gctccgcaat gcttttgtag
    20341 atcatctcta gatctctttt ttgcctgcaa gcccgggtaa tgcgcccaca agttctgctt
    20401 aatcaattga aaaaacatct tttttttaaa gatgtgttcg atgctgtgta ccagtgctca
    20461 cagagatcta ctttttcgcg aaaaagtaga tctctaccgc ccaacgaaaa gcatgaaagc
    20521 gatcacgaat cccattgggt cgtcatgttc tgccgatcgc atcttcctat cctcgctcca
    20581 ggcctgccgc ataaccaatc aggcttccta cttacagaat tgagaaaaga ggatgtggaa
    20641 atgctcagtc aaattcaacg ctttggcggc gcgatgttca cgccagtgct gctgtttccc
    20701 ttcgccggga ttgtggtggg tcttgccatc ttgctgcaaa acccgatgtt tgtcggggaa
    20761 tcactgaccg atccgaacag tttattcgcg caaatcgtac acattattga agagggcggt
    20821 tggacggtat tccgtaatat gccgctgatt tttgctgtcg gtttacccat tggccttgct
    20881 aagcaagcgc aggggcgtgc ttgtctggcg gtgatggtga gtttcctgac ctggaactat
    20941 ttcatcaatg cgatgggaat gacctgggga agctacttcg gcgtcgattt cactcaggac
    21001 gcggtggcag gtagcggtct gacaatgatg gccgggatta aaaccctcga taccagcatt
    21061 atcggcgcaa ttatcatttc cggcattgtg acggcgctgc ataaccgtct gttcgataaa
    21121 aaactgccgg tttttctcgg cattttccag gggacgtctt atgtggtgat tatcgccttc
    21181 ctggtgatga tcccctgtgc ctggctgaca ttgctcggct ggccaaaagt acaaatgggg
    21241 attgaatctc tgcaagcgtt cctgcgttcg gcgggtgcgc ttggggtgtg ggtttacacc
    21301 ttcctcgaac gtattctgat cccaaccggt ttacaccact tcatctacgg accgtttatc
    21361 tttggtccgg cagctgttga aggcggaatt cagatgtact gggcgcagca tctgcaagag
    21421 ttcagtttga gcgccgagcc gctgaaatcg ttgttcccgg aaggaggttt tgccctgcac
    21481 ggtaactcaa aaatctttgg tgccgtgggc atttctttag cgatgtactt cactgccgca
    21541 ccggaaaatc gggtaaaagt ggcgggtttg ctgattcccg caaccttaac cgccatgctg
    21601 gtgggaatta ccgaaccgct ggaatttacc ttcctgttca tttcaccgtt gctgtttgcg
    21661 gtacacgctg tgcttgcggc ctcaatgtcg accgtgatgt atctctttgg tgtggtgggc
    21721 aacatgggcg gaggtctgat tgaccaggtt ttaccgcaaa actggatccc gatgttcagc
    21781 aaccacgcgg atatgatgct gacccaaatc gccattgggt tgtgctttac cctgctgtac
    21841 ttcgtggttt tccgcaccct gattctgcaa ttcaacatgt gcacgccggg acgtgaagat
    21901 gcggaagtga aactctactc aaaagccgaa tacaaagcct cgcgaggcca aaccaccgcg
    21961 gcagagccaa aaaaagagct ggatcaggct gccggtatcc tgcaagccct gggcggggtc
    22021 ggcaatatct ccagcattaa caattgtgcg acgcgtctac gcattgcact gcatgacatg
    22081 tcacaaacgc tggatgacga agtctttaaa aagctgggag cgcacggcgt cttccgtagt
    22141 ggcgatgcca ttcaggtgat cattggtctg catgtatccc agctgcgtga acagctcgat
    22201 agcttaatta attctcatca atcagcagaa aatgttgcca ttacggaggc agtataatga
    22261 ccaaattctc agtggttgtc gcaggcggtg gaagcacctt tacgccaggc atcgtgttga
    22321 tgctcctggc gaatcaggac cgtttcccgc ttcgtgcact gaaattttat gataacgatg
    22381 gttcgcggca ggaagtgatt gccgaagcct gtaaagtcat ccttaaagaa aaagcgccgg
    22441 atattgcgtt tagttacacc accgatcctg aagtggcgtt cagcgacgtt gattttgtca
    22501 tggcgcacat ccgcgtcggc aaatacccga tgcgcgaact ggatgaaaaa atcccgctgc
    22561 gccacggcgt tgttggtcag gaaacttgcg gacccggcgg aatagcgtac ggcatgcgtt
    22621 ccattggcgg cgtcctggaa ctggtggatt atatggaaaa atattcacca aacgcctgga
    22681 tgctcaacta ctccaacccg gcagcaattg ttgcagaagc cacgcgtcgt ctgcgcccga
    22741 atgcgaaaat cctcaacatc tgtgacatgc caatcggtat tgaaagccgg atggcgcaaa
    22801 ttgttgggct gcaagatcgc aaacagatgc gcgtgcgcta ctacggcctg aaccactttg
    22861 gctggtggac atcaattgaa gatttgcagg gcaacgacct gatgcaccag ctgcggcaat
    22921 atgtctctaa gcacggttat gttccaccgc agcaagatac acatactgaa gcgagctgga
    22981 acgacaccta tgcaaaagcg cgggatgtcc aggcactgga cccggataca ttgccgaaca
    23041 cctatctgaa atattatctc ttcccggatt atgtcgttca gcattccaac cctgaacata
    23101 ctcgcgcgaa tgaggtaatg gaacatcgcg agaaacaggt tttcgatgct tgccgcgcca
    23161 ttacggcggc aggaaattca gcggcgggca agctggaaat tgacgaacat gcgtcataca
    23221 tcgtcgatct ggcggcggca attgccttca acactcagga gcggatgttg ctgattgtgc
    23281 ctaacaacgg ggcaatccat aactttgatg atgaagctat ggtcgagatc ccgtgtctgg
    23341 tcgggcacaa cggaccagaa ccactggtgg tcggcgatat cccgcagttt cagaaagggt
    23401 taatgagtca gcaagtggcg gtggaaaaac tggtcgtgga cgcctgggaa cagcgttcat
    23461 atcagcacct gtggcaggcg attacgttgt cgaaaacggt accgagcgcc tcggtcgcca
    23521 aagctattct ggatgagttg ctggaggcca acaaagcgta ctggccagag ttacgttaat
    23581 tgtgtggact ggcatccgtt gggtgctggt ccgtattctt tagcacggct gaaggcgcat
    23641 aaccaaattt atgtttaaag gctttgctga aatgaaagga gtcgaagaag ttgagcgtat
    23701 cagcgacttc gccaaccgag tttccttgtt gctttaatag cgatagcgcc agatccagac
    23761 gcgcatcgag gtaatattct ttcggcgtct ttccggtata gcgtaaaaac agacgacgca
    23821 accaggcttc gctacagggg atcgtggcag ccatatcggc tacgctccag cgttgttgca
    23881 gactggcatg taaagtggca attaattttt caatttgccg ccgttgtgga tcttttttac
    23941 cgtctgcgta cataagacaa atccactgat aaataatttt cgtcagaaaa gcgactgcca
    24001 gattattttt tattgcttct ggtgaagtta ttaactcagc aacttcggtg agttcctgat
    24061 tataaatttc gccattgtaa ataacgcttt gctgaccgat gggaatatcc atcatactgg
    24121 tgggggtaaa ttccatccag tattgttccc agactaaacc ttcacagtga taagagtgaa
    24181 tatccattgg ctttaaaaat ataatacagt tgccatgcag tgttatttgt tcgccattct
    24241 ttaaaagaat cttcccacat ccttgtacgg tatacaccgc aacccatgag ttggcgataa
    24301 gcggtttctt cttgtcccgt ggccagatca cccggtaatg ctcgtcggcg taggtatgcc
    24361 agagcgagat cagagaaatt ccgctggaga tcacattttc gttaatcaat aaaggaatat
    24421 tgtatggagt ttcgtttttt acatctgaag tttgcaattt tccattcatt ttaatatccc
    24481 tccggcgaat tatgctcaca acctgaactt aacagaaaac aatccgttca gggatacgac
    24541 gtttgtattt taagaatctg actgcctgac ccgacgcatt ttacctctcc ctatattcat
    24601 gcgtccggga cataaacata aataagggct atgagatgaa ttcgttacaa atcttgagtt
    24661 ttgtcggttt tacgctgctg gtggcgatca tcacctggtg gaaggttcgc aaaacagata
    24721 ccggatcgca acaaggctat tttcttgccg gacgttcact aaaagcgccg gttattgccg
    24781 cttcgttaat gttaaccaac ctttccacgg aacaactggt tggtctttcc gggcaggcct
    24841 acaaaagcgg catgtcggtg atgggctggg aagtgacttc agcggtgacg ctgatcttcc
    24901 tcgcgctaat ctttttaccg cgctatctga agcgcggcat tgccaccatc cccgattttc
    24961 tggaggaacg ttatgataaa acgacgcgta tgatcatcga cttctgcttc ctcattgcca
    25021 ccggcgtctg ctttctgccg attgttctct attccggcgc gttggcgctc aacagcctgt
    25081 ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc tatctggcta ctggtaattt
    25141 tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg actgcgcgca atggcagtgg
    25201 cggactccat caacggtatt gggctggtta tcggcgggtt gatggtgccg gtatttggct
    25261 tgatcgcgat gggcaagggc agctttatgc agggcattga gcaaattacc accgttcacg
    25321 ccgagaaatt aaactcagtc ggtggcccga ccgatccctt gccgattggc gcggcattta
    25381 ccggtttaat tctggtgaac accttttact ggtgtacaaa tcagggcatc gtgcaacgca
    25441 cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc gctgttaacg gcggtgctga
    25501 aaatgctcga cccgctggta ctggtgctgc cagggttgat tgcgtttcat ctgtatcagg
    25561 atctaccgaa agccgacatg gcctacccga cgctggtcaa taacgttctg ccagtgccac
    25621 tggtgggttt cttcggcgcg gtgttatttg gtgcagtgat cagtaccttc aacggctttc
    25681 tgaatagcgc cagtacgtta ttcagtatgg gtatttaccg tcgcatcatt aaccagaatg
    25741 ccgagccgca gcagctggtc accgtcgggc gcaaatttgg tttctttatc gctatcgttt
    25801 cggtgctggt agcgccgtgg atcgccaacg cgccgcaggg gctgtatagc tggatgaaac
    25861 agctcaacgg catttacaac gtgccgctgg ttaccatcat cattatgggc tttttcttcc
    25921 tgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat tggcataatc agctacatca
    25981 ccatcaacta tctggtgaag ttcgacttcc atttcctcta tgtgctggcc tgtacgttct
    26041 gcatcaacgt ggtcgtgatg ctggtgatcg gttttatcaa accgcgcgcc acgccgttca
    26101 ccttcaaaga tgcgtttgcg gtggacatga aaccgtggaa aaacgtcaag atcgcgtcaa
    26161 ttggcatcct gttcgcgatg attggcgtct atgccgggct ggctgaattc ggcggctacg
    26221 gtacgcgctg gttagcgatg atcagttatt tcattgccgc cgtagtgatt gtctacctga
    26281 tttttgacag ctggcggcat cgtcacgacc cagccgtaac ctttactccc gacgcgaagg
    26341 atagcctatg aaacgcccca attttctgtt catcatgacc gatacccagg cccccaatat
    26401 ggtcggttgc tatagcggta aaccgctgaa tacgcaaaat attgatagtc tggcggcgga
    26461 aggtattcgc tttaattccg cctacacctg ttcaccggtt tgtacaccgg cgcgcgccgg
    26521 gctgtttacc ggtatctacg ctaaccagtc cgggccgtgg accaacaacg tcgcgccggg
    26581 taaaaacatc tccactatgg ggcgctactt taaggatgcc ggctatcaca cctgttacat
    26641 cggcaaatgg catctcgacg gtcatgacta tttcggcact ggcgagtgtc cgccggagtg
    26701 ggacgctgat tactggttcg atggggcgaa ctatcttagc gaactgacgg aaaaagagat
    26761 tagcctgtgg cgcaatggcc taaacagcgt cgaagattta caggcgaacc atattgacga
    26821 aaccttcacc tgggcgcacc gcatcagcaa tcgagcggtg gattttctgc aacagcccgc
    26881 gcgcgccgag gaacccttcc tgatggtggt ttcgtatgat gagccgcatc acccgttcac
    26941 ctgtccggtg gagtatttag agaaatacgc tgatttttac tacgagctgg gcgagaaagc
    27001 acaggatgac ctggcgaaca aaccagaaca tcaccgctta tgggcgcagg cgatgccatc
    27061 gccagtcggt gatgacgggc tttatcacca tccgctctat tttgcctgta atgactttgt
    27121 tgatgaccaa atcggacggg tcatcaatgc cttaaagcca gagcaacgtg aaaatacgtg
    27181 ggttatttat acctccgatc acggcgaaat gatgggcgca cataagctga tcagtaaagg
    27241 ggcggcgatg tatgacgaca tcacccgcat tccgctgatc atccgttcgc cgcaagggga
    27301 gcggcgacag gtcgatacgc cagtcagtca tatcgattta ctgccgacaa tgatggcgct
    27361 ggcagatatt gaaaaaccag agattctgcc gggggaaaat atccttgcag tgaaagagcc
    27421 acgcggcgtg atggtggaat ttaaccgcta cgagattgag catgacagct ttggcggttt
    27481 tattccggtg cgttgctggg tgacggatga ctttaaactg gtgctcaacc tcttcaccag
    27541 tgatgaactt tacgatcgcc gtaatgatcc aaatgaaatg cataacctga tcgatgatgc
    27601 ccattttgcc gacgttcgca gcaaaatgca tgatgcctta ttggattata tggacaaaat
    27661 tcgcgatccg ttccgcagtt accaatggag cctgcgtccg tggcgtaaag atgcacagtc
    27721 gcgctggatg ggggcatttc gtccacgccc acaagatggt tattcgccag tggtacgcga
    27781 ctatgacacc ggcctgccga cacaaggggt gaaggtggaa gagaaaaaac agaagttctg
    27841 acgataatgc ccgctgatga acacccggcg ggcattattc aggctgctta aaccagtgaa
    27901 ccgccagtca ggaaggaaaa gataaggaat atcagcaaaa tgctgcacac gatgccgata
    27961 ccaaaaagca aggtatggaa agccagcgtc ccgccgccaa aaacccgcgc gccgcgaatg
    28021 ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc agcaatggcc
    28081 agtgcgatac cgctttgatc gaggatttta tcgacatcca cgtcctcttt tctctccacg
    28141 gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc agccgttttg
    28201 tccgccacga tgctttcaag cgttggcggt ggtgcggaaa aagggccgaa tgaaaagtgg
    28261 ataatcccca tcattaaagc gatggcaccg aatagcatgc ccaatgaact gattttattt
    28321 tgcgctatga tacccatctg acctccttgt agcggatggc aaaagcatat caggaaacgg
    28381 gcggacgaaa ttcttaaaaa aaaccgctaa aggtagagcg ttagtgaaat tgcaaaggag
    28441 caacaatgaa aatttcccgc ctcggagaag cgcctgatta ccgcttctcg ctggctaatg
    28501 agcgtacttt tctggcgtgg atccgtactg cactaggatt tctggcggcg ggggttgggc
    28561 ttgatcaact tgcgccagat ttcgccacgc ccgtcattcg cgaactgctg gcgttgttgt
    28621 tgtgtctgtt ttccggcggg ttagcgatgt atggctactt gcgctggtta cgcaatgaaa
    28681 aagcgatgcg tctgaaagag gacctgccct acaccaacag cttattaatc atcagcttaa
    28741 tattgatggt cgtagcagtg atcgttatgg gactggtgtt gtatgccgga tagccgcaaa
    28801 gccagacgca ttgccgaccc agggctgcag ccggagcgta catcactggc gtggtttcgt
    28861 accatgctgg gctacggcgc gttgatggcg ttggctatca aacacaactg gcaccaggcg
    28921 ggcatgttat tctggatttc gattggcatc ctcgccatcg tggcgctgat cctctggcac
    28981 tacactcgta atcgcaattt aatggatgtc acgaatagcg atttttccca atttcacgta
    29041 gtccgtgaca aatttttgat ctccctcgcg gtgttatctc tcgcaatact gtttgctgta
    29101 acgcatatac atcaacttat cgtatttatt gagagagtcg catgacagga agtcaggtca
    29161 ttgatgctga agaggacagg cataaactgg ttgtagagta caaggatgct ctgcaacctg
    29221 ccgattttta tcacaatttc aaacaacggg gcatccgctc cgtacagctt attccccatc
    29281 ttgaatttga tgaccggggc gatatgacgg ccgcttcggt gacagcagag ctgtggggga
    29341 aatttttaat tgccctgttt gagtgttggg tgcgggcaga tatcagtcgt atctcgattg
    29401 agctttttga cgccacatta caaaaatggt gtggcagcga aaacccgcaa ccgcgccgtg
    29461 gctgccaggc gtgcgactgg catctgctgt gtccgcacgc tcgggaggag acgccagaca
    29521 gcgtgctttg tgcgggctat caggcgtttt attcttactc tgcgccgcat atgcgggtga
    29581 tgcgtgatct gataaagcag catcgttctc ccatggagct gatgacgatg ctgcggtgac
    29641 gtgagcttaa acgggctgcc cctgatgcga catccgcgtc gccagcggta gccagcacag
    29701 cacgatcaac aatcccatta aggtcatcaa caaccccagg ctgccctgac cggtttgcgg
    29761 caacatcgca gagagcgacg ccagcacgcc ggaaccaatg ttttgtagac cgccgaccag
    29821 cgcgccagcc gtgcccgcca ggaaagggaa cggctccatc gcgccgctgg tcgccagcgg
    29881 aaacagcatc ccggcaccga aaaagaacag cgcggcggga acgagcagcg tccagacatt
    29941 catcacgcca aaccagtcgg ggatccacat cagtaagcca gccagcaggc agcagataac
    30001 cgactgccac atcaacgttg agaagcgttt attaggacgt ccggcaaacc atgccccaaa
    30061 aaatgccgcc ggaatcggca gaataaacaa aatactgacc gtcatactgc tcagccctaa
    30121 caccgcgccc atcagcacgc cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc
    30181 aatcagcatc agcaaataac agttaaaacc gctgttaccg aaaagcgttt tataactggt
    30241 aagcaggcgc gtgcgcggtg cgtcgaccgg acgcgtttcc ggcatccagc gggccatact
    30301 gaaggtcaca ccggcacaaa gtaccaacaa aaagagataa caggcgcgcc agttccacat
    30361 cgtatccagc agaccgccga ttagcggtgc gagcaacgga ctgacaagaa ttcccatgtt
    30421 taacaggctg ttagcgtggc gcaactgtgt ccgttcatat aaatcacgcg gcaaagtacg
    30481 cgccattacg ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg caatcaatac
    30541 tgtcaaactg gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg acattccgac
    30601 gaggatcacc ggtcggcgac ccacacggtc ggaaatcggg ccataaaaca gctgtgagac
    30661 accgtaagtc agcagataag cgcccattac gctctgcacc gccccttcac gaacgttgag
    30721 atcgcgcgcc atatcggcaa tagctggaat ataaatggtt tgcgccatct gaccgacggc
    30781 cacgagtaat accaacatca ataacaaatt gatgtttcta tgccttttca ttatcacgga
    30841 tgcttttata aaaataatta gaataagtca ctacgcattc ccataaatgc gggagaggaa
    30901 tctatcatat tgataaatgt tagccagata tacgccagga atggggaatt gtttagcggc
    30961 agaatatgta aacaaaagcg gcaataaatg ttgccgggag ggatggtagg tcggataaga
    31021 tgcgtcagca tcgcatccga caccaaccca cacgctaaat ataaaatggg agcggttacc
    31081 cgctcccctt tagtgcgact tgaatctgaa ttacttcagg tatttcagaa cagcatcaag
    31141 cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca ccaggctcat
    31201 acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct gcctgttgcc
    31261 agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc tcgttgacat aacacagtgt
    31321 gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact tagtgcgccg
    31381 ggtaacgatg cggcccccgc ataacacatt gcgtacagtg atattataga agcttgctgt
    31441 ataaataaac agtaatattt ggacaaaacg caaactgtga tcgatactca actatgcact
    31501 aaatacgtca aaattcgtgc cgaaattgcg cgttttgcgc ggaacacgta tactttctgt
    31561 gttgacataa tacagtgtgc tttgcggtta ccagccgcgg gcggctgacg aaacctcgct
    31621 ccggcggggt tttttgttac ctgcagtaca gtacaaaacg tgatcaaccc ctcaattttc
    31681 ccttgctgaa aaattttcca ttgtctcccc tgtaaagctg tgcttgtata aatattgtta
    31741 aacacaaaac caacaaggtc cccaatggct acttccatgc tcaacgcaaa actactacca
    31801 actgcgccat ccgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg
    31861 tagggactgg aacaacacac gattccaaaa ccccgccggc gcaaaccggg cggggttttt
    31921 cgtttaagca cctcccggaa agtcggccca gaagaaaagg actggagcat ggcaagttcg
    31981 ggcacatcat cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttca tttcctggaa
    32041 cagcagggca ttaagattgt gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat
    32101 gccttaagcc aaagcacgca aatccgccat attctggctc gccatgaaca gggcgcgggg
    32161 tttatcgctc agggaatggc gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc
    32221 ggaccggggg cgactaacct ggtgaccgcc attgctgatg cgcggctgga ctccatcccg
    32281 ctgatttgca tcactggtca ggttcccgcc tcaatgatcg gcactgacgc cttccaggaa
    32341 gtcgacacct acggcatctc tatccccatc accaaacaca actatctggt cagacatatc
    32401 gaagaactcc cgcaggtcat gagcgatgcc ttccgtattg cgcaatcagg ccgccctggc
    32461 ccggtgtgga tagacattcc taaggatgtg caaacggccg tttttgagat tgaagcacag
    32521 cccgcggtgg cagaaaaagc cgtcgcgccc gcctttagcg aagaaagcat tcgtgacgca
    32581 gcggcaatga ttaatgctgc caaacgcccg gtgctttatc tgggcggtgg tgtgatcaat
    32641 gcgcctgcga gagtgcgtga actggcggag aaagcgcagc tgcctaccac aatgacttta
    32701 atggcgctgg gcatgttgcc aaaagcgcat ccgttgtcgc tgggtatgtt ggggatgcac
    32761 ggtgtgcgca gcaccaacta tattttgcag gaggcggatt tgttgattgt gctcggtgcg
    32821 cgttttgatg accgggcgat tggcaaaacc gagcagttct gcccgaatgc caaaattatt
    32881 catgtcgata tcgaccgcgc agagctgggt aaaatcaagc agccgcacgt agcgattcag
    32941 gcggatgttg atgacgtgct ggcgcagctg atcccgcagg tggaagcgca accgcgtgca
    33001 gagtggcacc agttggtagc ggatttgcag cgcgaattcc cgtgtccaat cccgaaagcg
    33061 tgcgatccgt taagccatta cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat
    33121 gcgattatca ccaccgatgt gggtcagcat cagatgtgga cggcgcaggc ttatccgctc
    33181 aatcgcccac gccagtggct gacctccggc gggctgggca cgatgggttt tggcctgcct
    33241 gcggcgattg gcgcggcgct ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgat
    33301 ggcagcctga tgatgaatat tcaggagatg gcgaccgcca gcgaaaacca gctggatgtt
    33361 aaaatcattc tgatgaacaa cgaagcgctg gggctggtgc atcagcaaca gagtctgttc
    33421 tacaagcagg gcgtttttgc cgctacctat ccgggaaaaa tcaactttat gcagattgcc
    33481 gccggattcg gcctcgaaac ctgtgatttg aataacgaag ccgatccgca ggctgcattg
    33541 caggaaatca tcaatcgccc tggtccggcg ctgatccatg tgcgcattga tgccgaagaa
    33601 aaagtgtacc cgatggtgcc gccaggtgcg gcgaatacag aaatggtggg ggaataagcc
    33661 atgcaaaaca caactcatga caacgtgatt ctggaactca ccgttcgcaa ccatccgggc
    33721 gtaatgaccc acgtttgtgg cctttttgcc cgccgcgctt ttaacgttga aggcattctt
    33781 tgtctgccga ttcaggacag cgacaaaagc catatctggc tactggtcaa tgacgaccag
    33841 cgtctggagc agatgataag ccaaatcgat aagctggaag atgtggtgaa agtgcagcgt
    33901 aatcagtccg atccgacgat gtttaacaag attgcagtgt tttttcagta aggagcgaca
    33961 ttaaaccgct cgggggagtt actgatgagc gatttttgat agatccgtgc agatatatag
    34021 ggtgaaattg tgccagagac catctcaggc acaatttatt agcccaggac gttaatttaa
    34081 gtcgcagcac cggtaacctg ggggggacag ttagggctaa aatttccagc gatggtgtta
    34141 aattctgatt gctgtctata ccagtggtca tgaccagcat tctattaatt aatggttcat
    34201 cgtgaggtat attatggcaa gtcctataaa cagcggaata atgatggcaa atctctgccc
    34261 ttccaccttt ttggataaaa atagaaatgt cgctgctgag cttgatatta aaaataacga
    34321 gaaaaaatat tcacctggca gtaatttcgc aaaatggatg ttgcaggaaa taaagcgctt
    34381 aatactaaac atcatgtccg gttctcggag tattaacacc gatattcttg actatttcca
    34441 ccctatgcca ggcacggaaa ataatggtaa tcgtacctgg gtggcggcaa ctggagagga
    34501 tgaatatatc gaaatcaaac aaacaggtga taagtctttt aatattacac tggttggcag
    34561 agataaacct tcacgaaaag aaattccata ttcaggagtg gctgtagcga ctattattaa
    34621 atcgttgtct gaaaaaactt ctgcgttaga aacacattcc gctgacacgg ttttgaggaa
    34681 gaaattagtt aatagtattg ttatgaag
//
LOCUS       DAZGXS010000035        34638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGXS010000035 DAZGXS010000000
VERSION     DAZGXS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            18..2444
                     /gene="ynfE"
                     /locus_tag="ACHKG5_001863"
     CDS             18..2444
                     /gene="ynfE"
                     /locus_tag="ACHKG5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP0867975.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAATAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2505..4928
                     /gene="ynfF"
                     /locus_tag="ACHKG5_001864"
     CDS             2505..4928
                     /gene="ynfF"
                     /locus_tag="ACHKG5_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0867976.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEVPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4939..5556
                     /gene="ynfG"
                     /locus_tag="ACHKG5_001865"
     CDS             4939..5556
                     /gene="ynfG"
                     /locus_tag="ACHKG5_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP0867977.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5558..6412
                     /gene="ynfH"
                     /locus_tag="ACHKG5_001866"
     CDS             5558..6412
                     /gene="ynfH"
                     /locus_tag="ACHKG5_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0867978.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6455..7069
                     /gene="dmsD"
                     /locus_tag="ACHKG5_001867"
     CDS             6455..7069
                     /gene="dmsD"
                     /locus_tag="ACHKG5_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0867979.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7227..8519
                     /gene="clcB"
                     /locus_tag="ACHKG5_001868"
     CDS             7227..8519
                     /gene="clcB"
                     /locus_tag="ACHKG5_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0867980.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHGDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8472..9167)
                     /gene="bioD"
                     /locus_tag="ACHKG5_001869"
     CDS             complement(8472..9167)
                     /gene="bioD"
                     /locus_tag="ACHKG5_001869"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0867981.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9292..10512)
                     /gene="mlc"
                     /locus_tag="ACHKG5_001870"
                     /gene_synonym="dgsA"
     CDS             complement(9292..10512)
                     /gene="mlc"
                     /locus_tag="ACHKG5_001870"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0867982.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10647..11540)
                     /gene="ynfL"
                     /locus_tag="ACHKG5_001871"
     CDS             complement(10647..11540)
                     /gene="ynfL"
                     /locus_tag="ACHKG5_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0867983.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11647..12900
                     /gene="ynfM"
                     /locus_tag="ACHKG5_001872"
     CDS             11647..12900
                     /gene="ynfM"
                     /locus_tag="ACHKG5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0867984.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYISYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(12949..13068)
                     /locus_tag="ACHKG5_001873"
     CDS             complement(12949..13068)
                     /locus_tag="ACHKG5_001873"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0867985.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13324..13632
                     /gene="asr"
                     /locus_tag="ACHKG5_001874"
     CDS             13324..13632
                     /gene="asr"
                     /locus_tag="ACHKG5_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0867986.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            13725..13808
                     /locus_tag="ACHKG5_001875"
     CDS             13725..13808
                     /locus_tag="ACHKG5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0867987.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13908..14729
                     /gene="ydgD"
                     /locus_tag="ACHKG5_001876"
     CDS             13908..14729
                     /gene="ydgD"
                     /locus_tag="ACHKG5_001876"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0867988.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14768..15097)
                     /gene="mdtI"
                     /locus_tag="ACHKG5_001877"
     CDS             complement(14768..15097)
                     /gene="mdtI"
                     /locus_tag="ACHKG5_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0867989.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15084..15449)
                     /gene="mdtJ"
                     /locus_tag="ACHKG5_001878"
     CDS             complement(15084..15449)
                     /gene="mdtJ"
                     /locus_tag="ACHKG5_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0867990.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15861..16895
                     /gene="tqsA"
                     /locus_tag="ACHKG5_001879"
     CDS             15861..16895
                     /gene="tqsA"
                     /locus_tag="ACHKG5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP0867991.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16920..18308)
                     /gene="pntB"
                     /locus_tag="ACHKG5_001880"
     CDS             complement(16920..18308)
                     /gene="pntB"
                     /locus_tag="ACHKG5_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0867992.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18319..19851)
                     /gene="pntA"
                     /locus_tag="ACHKG5_001881"
     CDS             complement(18319..19851)
                     /gene="pntA"
                     /locus_tag="ACHKG5_001881"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0867993.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20375..21319
                     /gene="ydgH"
                     /locus_tag="ACHKG5_001882"
     CDS             20375..21319
                     /gene="ydgH"
                     /locus_tag="ACHKG5_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0867994.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21505..22887
                     /gene="ydgI"
                     /locus_tag="ACHKG5_001883"
     CDS             21505..22887
                     /gene="ydgI"
                     /locus_tag="ACHKG5_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0867995.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22924..23646
                     /gene="folM"
                     /locus_tag="ACHKG5_001884"
     CDS             22924..23646
                     /gene="folM"
                     /locus_tag="ACHKG5_001884"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0867996.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23643..23978)
                     /gene="ydgC"
                     /locus_tag="ACHKG5_001885"
     CDS             complement(23643..23978)
                     /gene="ydgC"
                     /locus_tag="ACHKG5_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0867997.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24107..24826
                     /gene="rstA"
                     /locus_tag="ACHKG5_001886"
     CDS             24107..24826
                     /gene="rstA"
                     /locus_tag="ACHKG5_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0867998.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24830..26131
                     /gene="rstB"
                     /locus_tag="ACHKG5_001887"
     CDS             24830..26131
                     /gene="rstB"
                     /locus_tag="ACHKG5_001887"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0867999.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26207..27136
                     /gene="tus"
                     /locus_tag="ACHKG5_001888"
     CDS             26207..27136
                     /gene="tus"
                     /locus_tag="ACHKG5_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0868000.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27133..28536)
                     /gene="fumC"
                     /locus_tag="ACHKG5_001889"
     CDS             complement(27133..28536)
                     /gene="fumC"
                     /locus_tag="ACHKG5_001889"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0868001.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(28679..30325)
                     /gene="fumB"
                     /locus_tag="ACHKG5_001890"
     CDS             complement(28679..30325)
                     /gene="fumB"
                     /locus_tag="ACHKG5_001890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0868002.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30524..31699
                     /gene="manA"
                     /locus_tag="ACHKG5_001891"
     CDS             30524..31699
                     /gene="manA"
                     /locus_tag="ACHKG5_001891"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0868003.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            31800..33308
                     /gene="ydgA"
                     /locus_tag="ACHKG5_001892"
     CDS             31800..33308
                     /gene="ydgA"
                     /locus_tag="ACHKG5_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0868004.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGYPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(33353..34617)
                     /gene="uidC"
                     /locus_tag="ACHKG5_001893"
                     /pseudo
     CDS             complement(33353..34617)
                     /gene="uidC"
                     /locus_tag="ACHKG5_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
BASE COUNT         8511 a         8928 c         8537 g         8662 t
ORIGIN      
        1 agaacaggga gtgagttatg tccaaaaatg aacgaatggt gggcatcagc cgcagaacac
       61 tcgttaaatc taccgcgata ggttctctgg cgctggctgc aggcggtttt tctttgccgt
      121 tcaccctgcg cagtgcagcg acagcagtac aacaggccag cgaacaagtg gtctggggtg
      181 cctgttccgt caactgtggt agccgctgtg cacttcgtct acatgttaaa gataatgaag
      241 tggcctgggt ggaaactgac aacaccggca gcgatgagta tggcaaccat caggtacgcg
      301 cctgtttgcg cggtcgctcc atccgccggc gtattaatca tcccgatcgc ttgaattacc
      361 caatgaaacg cgtgggtaaa cgcggtgaag gcaaattcga acggattagc tgggatgaag
      421 ccctggatac tatcgccagt agcctgaaga aaaccgtcga acaatatggc aatgaggctg
      481 tatatattca gtactcttcg gggatcgttg gcggcaatat aacccgctct tcgccatcag
      541 cctcggcggt caaacgcctg atgaactgct acggcggttc actcaaccag tatggctcct
      601 acagcactgc gcaaatttcc tgtgccatgc cctacaccta cggcagtaat gatggcaaca
      661 gcaccacgga tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac ccggcagaaa
      721 cccgcatgag cggcggtggc atcacttatc ttcttgaaaa atcccgcgaa aaatcgaacg
      781 caaaaatgat tgttatcgat ccgcgatata ccgatacggc tgctggtcgt gaagatgaat
      841 ggcttcctat tcgcccgggc accgatgccg cgctggttgc gggtattgcc tgggtattga
      901 ttaacgaaaa tctcgttgat caaccttttc tcgataaata ctgcgtcggc tatgacgaaa
      961 aaaccttacc cgcagatgca cccaaaaatg gtcactataa agcctatatt cttggtgaag
     1021 gtgacgataa aacagcgaaa acgccgcagt gggcttcgca aattaccggt atcccggtgg
     1081 accgtatcat caaactggcg cgtgaaattg gcacagcaaa acccgcttat atctgccagg
     1141 gctgggggcc gcaacgccag gcaaacggcg agctgaccgc acgcgctatt gctatgttgc
     1201 ctattctgac gggcaatgtc ggcatcagcg gcggaaatag tggcgcgcgt gaatcgacct
     1261 ataccattac catagagcgc ctgccggtgc tggataatcc ggtcaaaacg tcaatttcct
     1321 gcttcagctg gacagatgct atcgatcatg gcccacaaat gacggcaatc cgcgacggtg
     1381 tccggggcaa agataaactg gatgtgccca ttaagttcat ctggaactac gcgggaaata
     1441 ccctggttaa tcagcattct gacatcaaca aaacgcatga aattctgcag gacgaatcga
     1501 aatgcgaaat gattgtggtc atcgaaaact ttatgacctc atcggcaaaa tatgccgaca
     1561 ttctgctgcc agacctgatg accgttgagc aggaagatat tattcctaac gactacgccg
     1621 gtaacatggg atatctcatt ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac
     1681 cgatttactg gatcctgagt gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt
     1741 tcactgaagg tcgcacgcag gaacaatggt tacaacatct gtacgccaaa atgcttgcca
     1801 aagatccggc gttaccgtct tacgacgaac tgaaaaaaat gggtatctat aagcgtaaag
     1861 atcccaatgg tcattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac
     1921 ttaaaacacc ttccggtaag attgaaattt attccagcaa gctggcggaa attgcccgta
     1981 cctgggaact ggaaaaagat gaagtgataa gcccactgcc agtttatgcc tcaacctttg
     2041 aaggctggga ctcccctgaa cgtagtacct ttccactgca actgtttggt ttccattaca
     2101 aatcccgtac tcactcgacc tacggcaaca ttgatgtcct gaaggctgcc tgccgtcagg
     2161 aggtgtggat caaccctata gacgcacaga aacgtgggat tgccaacggc gatatggtgc
     2221 gggtgtataa ccatcgtggc gaagttcggc taccagccaa agtgacacca cgcattctcc
     2281 ctggggttag cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa
     2341 tcgaccatgg cggatgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg
     2401 gaaacccgca gcacacgaat ctggtcgaga tcgaaaaaat ataacccact acaaccataa
     2461 attctggcat gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca
     2521 cagaggcgct catgaaggct gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg
     2581 gcagtcttgc gctggcaagc agtgctttca ctctgccatt ttcccaaatg gtccgagcgg
     2641 cagaggtacc ggtagaagag aaagcggtct ggagttcctg caccgttaac tgcgggagcc
     2701 gctgtctgtt acgtttgcat gtgaaagatg acaccgtgta ctgggtggag tctgatacga
     2761 caggtgacga cgtctacggt aatcatcagg ttcgtgcgtg tttacgcggg cgctctatcc
     2821 gccgacggat gaatcatcct gataggttga aatatcccat gaagcgcgtc ggcaagcgcg
     2881 gtgaaggtaa atttgaacgg ataagttggg acgaagccct ggataccatc agtgataatc
     2941 ttcggcggat cctgaaagat tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg
     3001 tagatggcgg aaacatcacc aactcaaacg tcccgtaccg tctgatgaac tcttgcggtg
     3061 gttttctcag tcgctatggc agctacagta ccgcacagat cagtgccgca atgagttata
     3121 tgttcggtgc caatgatggc aacagcccgg atgatatcgc caatacgaaa ctggtcgtta
     3181 tgttcggaaa taacccggca gaaacgcgga tgagcggtgg tggtgtcact tactacgtcg
     3241 agcaagcccg cgaacgttca aacgcacgca tgatcgtcat cgatccacgt tataacgaca
     3301 cagctgccgg gcgtgaagat gaatggctgc ccattcgccc tggcaccgat ggcgcactgg
     3361 cctgtgcgat tgcctgggta ctgattagtg aaaacatggt cgatcagcca tttctcgaca
     3421 aatattgtgt tggttacgat gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt
     3481 ataaagccta tattctgggc gaagggcctg acggcatagc taaaacgccg gaatgggcag
     3541 caaaaatcac cagcatcccg gcagaaaaaa ttatccagtt ggcacgagag atcggttcag
     3601 caaaacctgc ttatatttgt cagggttggg ggccacaacg acattccaac ggcgagcaaa
     3661 catcccgcgc tattgccatg ctttccgttc tcaccggtaa cgtcggcata aacggcggca
     3721 actcaggtgt acgcgaaggt agctgggatc tgggggtaga atggttcccg atgctcgaga
     3781 atcctgttaa aacgcagatt tccgtcttta catggacaga tgccatcgac catggtacgg
     3841 aaatgaccgc gacccgtgat ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt
     3901 ttttatggtg ctacgccagt aacacattga tcaatcaaca tggcgacatc aatcacacca
     3961 atgaggtgct tcaggatgac agcaagtgcg agatgattgt tggcattgac cacttcatga
     4021 cggcctcggc taagtattgc gatatcctgt tgcccgacct gatgccaaca gagcaagaag
     4081 accttatctc tcatgaatct gcagggaata tgggctatgt gatcctcgcc caacccgcaa
     4141 cctcagcaaa atttgaacgc aaacccatct actggatgct gagtgaagtc gccaaacgct
     4201 taggaccgga cgtttatcaa acctttactg aaggtcgcag tcagcatgaa tggatcaaat
     4261 atctccatgc gaaaacgaag gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa
     4321 cgaccgggat ctttaagaaa aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc
     4381 gtgaagatcc acaggcaaac ccgttgaaaa caccttcggg gaaaatcgaa atttattctg
     4441 aacgactggc gaagattgca gatacctggg aattgaaaaa agatgaaatt attcatcccc
     4501 ttcctgcgta taccccaggt tttgatggct gggacgatcc cctgcggaaa acctatccac
     4561 tgcagttaac gggcttccac tacaaagcgc gtacccactc cagctacggc aatattgatg
     4621 tgttacagca ggcctgccca caagaggtgt ggatcaaccc cattgatgct caggctcgcg
     4681 gtatccgtca tggcgatacc gtgcgggtat ttaacaataa tggagaaatg ctgattgccg
     4741 caaaagtgac tccgcgtatt ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta
     4801 aagcggatat gtttggtgac cgggtcgatc acggcggcag tatcaatatt ctgacctctc
     4861 accgcccttc accgctggca aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa
     4921 aggtttaagg agtagcccat gaccacacaa tatggatttt ttatcgattc cagccgctgt
     4981 actggctgta aaacctgcga actggcgtgc aaagatttta aagatcttgg cccggaagtc
     5041 agtttccgcc gtatttatga atatgctggc ggcgactggc aggaagataa tggcgtctgg
     5101 caccagaacg tgttcgctta ttatctctcc atctcctgta accattgcga tgaccccgca
     5161 tgtacaaaag tctgccccag cggcgcaatg cataagcgtg aagatggctt tgtggtggtt
     5221 gatgaagatg tttgtatcgg ctgccgctac tgccacatgg cttgtccgta cggcgcgcca
     5281 cagtacaatg ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc
     5341 gaggggaaac aacccatatg tgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca
     5401 attgaagagc tgcgccagaa gcacggcact cttgctgccg tcgcgccact gccgcgcgcg
     5461 catttcacaa aacccaatat cgttatcaaa cctaacgcca acagccgccc gaccggcgat
     5521 accactggtt atctggctaa tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg
     5581 gccactggtt atctttaccg tattgggtca atgcgtagta ggcgcgctga ttgttagcgg
     5641 aattggctgg ttcgccgcca aaactgacgc agaccgtcag cacattgttc gtggcatgtt
     5701 tttcctctgg ctgttgatgg gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc
     5761 actgcgggcg tttaactcgc tgaatcgaat cggagcttct ggtctgagta acgagattgc
     5821 tgccggttcc atattttttg ccgttggcgg actatggtgg ctggtggcgg tcatcggtaa
     5881 aatgccgcaa gcgttgggga aactctggtt acttgtcagc atggcgctcg gcgtcatttt
     5941 cgtctggatg atgacctgcg tgtatcaaat cgacactgtg ccaacctggc ataacggtta
     6001 cactacgctg gctttcttcg tgacggtatt gttgagtggt ccaatacttg ctgccgcaat
     6061 tctgcgggca gcccgcgtta catttaatac cacaccattt gccatcatta gcgttctggc
     6121 gctgatcgct tgcgccgggg tgattgtgct gcaaggtttg tctctgtcgt ctattcactc
     6181 atccgtgcaa caagccagcg cactggtacc agattacgcc tccttacagg tctggcgtgt
     6241 ggtattgtta tgcgcaggtc taggatgctg gctttgtccg ctcatacgtc gccgtgaacc
     6301 tcacgttgcc ggacttattc tggggttaat cctcattctt ggcggtgaga tgatcgggcg
     6361 tgtgctcttt tatggcctgc acatgacggt aggaatggct attgcaggtt aacaaattgg
     6421 tgcggggcaa cccgcacatt tcaggatgtt aggaatgacc catttttcac agcaagataa
     6481 tttttctgtc gcggcgcgcg tgttgggcgc gctgttttat tacgctccag agagtgctga
     6541 agccgcacct ctggttgcgg tactcaccag tgacggctgg gaaactcagt ggcctctacc
     6601 agaagcgtca ttagcgccgc tggtgacggc ttttcagaca cagtgtgaag agactcacgc
     6661 ccaggcctgg cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc cgtgggggtc
     6721 ggtctggctg gatcgcgaat ctgtgctgtt tggcgattca acattggcac ttcgtcagtg
     6781 gatgcgcgag aaaggcattc agtttgaaat gaagcaaaac gaaccggaag atcattttgg
     6841 ttcactgctg ttgatggctg cgtggttggc ggagaatggt cgccagacag aatgcgaaga
     6901 attgctggca tggcaccttt ttccctggtc aacacgtttt cttgacgttt ttatcgaaaa
     6961 agcagaacac cctttctacc gtgcactggg tgaactggct cgcttaacgc tggcgcaatg
     7021 gcaatcacaa ctgttaattc ctgtcgcggt taaaccactc tttcgatagg atcaccgtaa
     7081 tattgccgga tggtgacgat tacgcgcgat tcggcaacaa tacgttacag tgtcacgttt
     7141 tttgtctctt aaagcacgca ctgcttttgc agctggcctc ttttgccgca aaatagtcgc
     7201 ccgtgtttca ttgcccattt ctgctcatgc atcatctaca cacctatccg gatctgcgca
     7261 ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc tttgccgttg
     7321 ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt cctcaataat gactccggca
     7381 gtctggtcaa tgcagcgaca aacctttccc cctggcgacg gctgctaact ccggcgctcg
     7441 gcggactggc ggcgggtttg ttgttgatgg gctggcagaa atttacccag caacgccctc
     7501 atgcgccgac cgattacatg gaagcgttgc aaaccgatgg tcagtttgat tacgcagcaa
     7561 gcctggttaa atcgcttgcc tctctgctgg tagtaaccag cggcagtgca attggtcgcg
     7621 aaggtgcgat gattctttta gctgcccttg ccgcctcctg ttttgcccaa cgttttacgc
     7681 cacgccagga gtggaaatta tggatcgcct gtggggccgc ggcgggaatg gctgcggcct
     7741 atcgtgcccc gcttgctggc agtttattta tagccgaagt gctgtttggc actatgatgt
     7801 tggcctctct cggcccggtg attatttccg ccgtcgtggc attgctggtt agcaatctga
     7861 ttaatcatgg cgacgcgtta ctctacagcg tacaactctc agtgacggtt caggctcgtg
     7921 actatgcgct gattatcagt acaggtgtgc tggcaggtct gtgcggacca ctgttgttaa
     7981 cattaatgaa cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg ccctggcaac
     8041 tggcactagg cgggttgatc gtgggtctgc tttccctgtt cacacctgca gtgtggggca
     8101 acggctatag caccgtacaa tcctttttaa ccgccccgcc actgttaatg atcattgccg
     8161 ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc gcacccggtg
     8221 gtgtctttac accgacgcta tttatcggtc ttgccattgg catgttgtat ggtcgtagtc
     8281 tgggattatg gttccctgat ggcgaagaaa ttacgctttt actcggattg accgggatgg
     8341 cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg atatgtgaaa
     8401 tgaccgggga gtatcagcta ctccccggtt tattgattgc ctgcgtaatt gcgtcggtaa
     8461 tctcgcggac gttacaccgt gactctatct accgccagca cactgcgcag catagctaag
     8521 cggatgtatt gccccagttc acgctgttcg gcgcgcggca gataaggcag ttcaccaatg
     8581 agcggtgccg gaagtttttt accgagcaca tcaatgattt ccgcataatg cgccagtcct
     8641 gggttgattc ggttagccac ccagccaatg agcggcagcc cgtcgttggc aatcgcctga
     8701 gctgttagca gtgcatggtt aatgcaacct tcctgaatac cgacaaccat caacaccggc
     8761 agttgttcct gcaccaccca ttcagagagt ggacgcaaat cattcatcag actacgccag
     8821 ccgccagtcc cttctaccac gacatgatcg actttttcgg tcaggtttgc caggccgttt
     8881 gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc tactttcttc ttcgcttaac
     8941 gcgataggat taactgcttc ataaggcagt tcgatggttg aaacactctg caacaccagg
     9001 gcatctttat tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac gggtttatat
     9061 cccgcaaccg tttttccctg ggaggctaac gcttgtagca atgcgcggga aaccaccgtt
     9121 ttccctacag aagtgtctgt accggtaata aagaaacgct tcagcatcac taactccacc
     9181 gttatgcttc acaaatataa accaggaaaa taattaacct tgaaagtcta agttatgctt
     9241 tcctggccca aattgagata gcgcaaattt tggtagaaca gttaaaaaat gttaaccctg
     9301 caacagacga atcaacaaag aaccgttata catcgcgtct tttaccagcg cagcccctgc
     9361 catcgtgccc tggttagaaa actgagtact ctcaacgcta atgtgctgac tatacgcagg
     9421 aagggcctgc tgacggatgc tatctgagat gaccgggaag aggatatctg ccgctttact
     9481 taacggtgag ccaatcagta ttttttgtgg gttaaataaa ttcaccatga tggcaagaat
     9541 gcgccctaca tgcgcgccca ccccggtaat gatgtctttt gccagcagat cgccgcgcaa
     9601 tgccgcctga cacaatgagt ccacggttaa cggttgccca tgtaacatcg agctcatgga
     9661 ttgattgaga cgcagctgtg ccagctcaag aatactgtcc acactggcga tggtttcgag
     9721 gcagccgtgg ttcccgcaat aacagcgttt accatatggg tctacctgtg tgtgacctat
     9781 ttccacgagg ctactgctgc ctgcgtgtag cagatgacca tcggtaatga cgcccgcccc
     9841 cacgttgtga tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg caccaaacaa
     9901 ggcctctgcc atcgtccatg cgctgatatc atgctgaata taaaccggaa cgccggtatg
     9961 ctgctccaga gcctcgccga gcggcatctc ttttacatcc tcgtagaacg gcatgcgatg
    10021 tacaatgcca ttttccgtat caataattcc cggcagggtt atggcaatcg aagttagacg
    10081 ctcaagtttt ttctggtggc ggataaaaaa ctgatcgata tgggaaatga tacgatccag
    10141 caatggcgag tcatctttta acgccagttc ctgcgactct tccaccacca gtttgctgct
    10201 cagatcgcgc agagcaagga aaatctcccc gcgactaatg cgcagagaaa gatagtgcca
    10261 ggcttcagtt tcaaccacca gccccaccgc cggacggcca cggttccccg cttctttgat
    10321 ttccagctct tgcaccaggt gtgcttcgag catctcacgg acaattttag tgatactggc
    10381 aggagccagc tgcgccagac gggaaagatc gatacgcgag actggaccaa gctgatcaat
    10441 caggcgataa accgcgcccg cgttggtctg ctttatttga tcaatgtgcc caggctggtt
    10501 ttcagcaacc accgcatact ccctatattt tcgcgctccg aaataatctg taggctatgg
    10561 tgaagcactt caatacgtgt cgtcaaattt ttacttaggc atgtgattaa cagcacattt
    10621 ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat tcagcagatg aatacgaaag
    10681 ttacgcgcag ctggactttg ttcatgatgt ttcggccaga ccaaccacat ttcagaaacc
    10741 gcatcctctt cagcaatcgg cacccagcgc atttcgttga gctgaacacg tttaaatgac
    10801 gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac cgatgatggt cattgcctcg
    10861 cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc gagaatatcg
    10921 tcatacagcc ctgtcccgac gtgcggatca aaaaagacaa agggttcttt cgccagttca
    10981 gccagcgtta cattcgggtt atttgccagg ggatgatcgt gcgggatcat cgccataagc
    11041 ggttcatgga cgatgactgc gtgttcaagc gtctccggta acgctgtgtt acgcagcaat
    11101 cccatatcca gcgttccttc aatgagcgga gcgatttgct cgcgagtgtt catttcgcgg
    11161 gtttgtaaat ggacatcggg ataatctcgg cgaaacagcg ataacgtatc ggacaccgcc
    11221 cgaataaaag gagccgacga agtaaaacca atgcgcaact cccccgcttc accctgatgc
    11281 agtctttcag cgcgagcggc agcgtcatcc accatagaca ggatttgccg actatctgca
    11341 agaaactgtt ttcctgctgc cgtcagcaat acactgcgat tggttcgtgc cagcagtcgg
    11401 gcaccaattt gttgctccag cgcctgaatc tgctgactta gcggcggttg cgaaatattc
    11461 aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa gtaacgcaga
    11521 tgacgaagtt caatattcat atttaaaaca tcttatttga gattattaat atattagaca
    11581 gaacaattcg attttcctaa cctatgtata agcctgatcc acaggcatat ttagcaagga
    11641 tttcaagtga gccgtactac aactgttgat ggcgctccgg caagcgacac tgacaagcaa
    11701 agcatttctc agccaaatca atttattaaa cgcggtacgc cgcaatttat gcgcgtcacc
    11761 ctggcgctgt tctctgccgg actggcaaca tttgcacttc tctattgtgt gcaacctatc
    11821 cttccggtgc tttcgcagga gtttggctta acgcccgcga acagtagtat ttcactgtcc
    11881 atttccacgg cgatgttggc tattggtttg ctgtttactg gccccctatc cgatgccatt
    11941 ggtcgcaaac cagtgatggt aacggcgcta ctattggcct ccatttgtac gttactttcg
    12001 acaatgatga ccagctggca cggcattttg attatgcgcg ccttgattgg gctttcgtta
    12061 agtggcgtgg cagctgttgg catgacttat cttagcgagg aaatccaccc cagtttcgtg
    12121 gccttttcga tggggttgta tatcagcggc aactcaattg gcggcatgag cggacgctta
    12181 attagtggtg tcttcacgga ctttttcaac tggcgaattg ctctggcggc tatcggttgt
    12241 ttcgcgctag cctcggcgtt gatgttctgg aaaatcctcc cggaatcacg ccattttcgc
    12301 ccgacttcgc tgcgacctaa gtcgttgttt atcaactttc gtctgcactg gcgtgaccgg
    12361 ggattaccgt tattgttcgc agaaggcttt ttgctgatgg ggtcgttcgt cacgctgttt
    12421 aattacatca gctatcggtt gatgctctcc ccctggcatg tcagtcaggc cgtggttggc
    12481 ttattatcgc tggcttattt gaccggtaca tggagctcac ccaaagccgg aaccatgacc
    12541 acccgctatg ggcgtggtcc agtgatgttg ttttcgacgg gggttatgct gtttggttta
    12601 ctgatgacct tattcagctc gctgtggctg atctttgccg gaatgttact cttctcagca
    12661 ggattcttcg cagcccactc agttgccagc agctggatcg gcccccgcgc aaaacgcgct
    12721 aaaggccagg cctcctcgct gtatctgttc agttactatc tggggtcgag tattgccggg
    12781 acgctgggtg gtgttttctg gcataactat ggctggaacg gcgtcggcgc atttattgct
    12841 ctgatgctgg tcattgctct gctggtcggg acgcgtttgc atcgtcgtct gcacgcctga
    12901 aaaataagtc cggactgcgg taaatacccg tccggactta ttgcctgctc aaaccaacgt
    12961 taatagccat cctaaaatag acgaagcgcc agccaattcc cgcagcgcgt ctagcgtcat
    13021 caggattata agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg
    13081 cgcagttatt ctaccgcttt gtaagtagta aaatagttaa cccgatcaag actactatta
    13141 ttggtagcta aattttcctt aagtcacaat acgttattat caacgctgta atttattcag
    13201 cgtttgtaca tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattcccagg
    13261 gatatagttc tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg
    13321 acaatgaaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgccgcc
    13381 tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgccg
    13441 gcgaaaacta cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg
    13501 gctaaaaagc atcataaaaa tacgaaagct gaacagaaag ccccagaaca aaaagcgcag
    13561 gcagcgaaga aacacgccaa gaaacacagc catcagcaac cggcaaaacc tgctgcacaa
    13621 cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg
    13681 tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc
    13741 gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc
    13801 tttcctgatc gtagtgatct gattttactc ccactttcat cccgtcccgt ctatagtatt
    13861 tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca
    13921 ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag
    13981 acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg
    14041 tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg gaaacggcca
    14101 gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg gcaggacact
    14161 gtttattgac gccaccaaag ggtaaagccg ataaagcagt ggcgctgcgt tttgtgtcaa
    14221 ataaaggtct ttggcgctat gacatccacg acatagaagg ccgcgttgat ccgacactgg
    14281 gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc gcgccgtggg
    14341 acttcggatt gattgtgcta cgtaatcccc cttctggcat tacgccgttg ccgttatttg
    14401 agggagataa agccgcgctt accgccgcat taaaagcggc aggtcgtaaa gtgactcagg
    14461 caggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg
    14521 gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt gacagcggtt
    14581 cgcctctgat gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc
    14641 ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg
    14701 acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgacag cgcgggcagc
    14761 gtcttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc
    14821 agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa
    14881 taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag
    14941 aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcgac
    15001 gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg
    15061 ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta
    15121 cgtgctttac gggtacctga tttgatcaac actatcccgg cgaccagggt ggttaacccg
    15181 gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat
    15241 aaaataccga taccttccca cagcgcataa gccacaccta aggctatttt tttaacagcg
    15301 aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt
    15361 ccctcactga cgctcgccca tttcatagac agcgtaccgg taatttctgt agcaatagcc
    15421 agacctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat
    15481 tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgtata gggcaagctt
    15541 aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt
    15601 ggatgaggta cgaagatggg taaatgtaga aaataacgtc ctgaacaaat tgtccataat
    15661 attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt
    15721 gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc acttctttac
    15781 ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta ttagcaaagg
    15841 aagaaaaaac ggggacaagc atggcaaagc cgctcatcac gctcaatggc ctaaaaatcg
    15901 tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc gccgagatca
    15961 tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg gtgcaacaca
    16021 tggtccgctg gcgtgtgccg cgtgtactgg cagtgtcgat tttgatgacc atcatcgtga
    16081 tggctatggt gttgctgtta gcttatctgg gttccacgct caacgagttg acgcggacgt
    16141 taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg ttgttgcaac
    16201 gcgtagggat tgacgtctca gttgaccaac tggcgcatta cattgatccg aacgcggcga
    16261 tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca atatttttat
    16321 tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga aaatttaagc
    16381 aaatgatggc gcgtccggtt gaagggatgg cggcgattca gcgtgcgatt gacagtgtgt
    16441 ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc gcatgggcga
    16501 tgctcgccgc cctcgatgtt cgcttcgctt ttgtctgggg attgctggcc tttgcgctta
    16561 attacatccc taatattggt tcagttctcg cggcaatccc ccctattgct caggtactgg
    16621 tgtttaatgg cttctacgaa gcgttgttgg tgctggcggg atatctgctg attaatctgg
    16681 tcttcggcaa tattctggag ccacgcatca tggggcgtgg gctgggactt tccacattgg
    16741 tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgcttt
    16801 ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca
    16861 tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt
    16921 tacagagctt tcaggattgc atccacgctg gctttggcgt caccaaacag catgtgggtg
    16981 ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta
    17041 aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc aatcggactc
    17101 ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta
    17161 tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact
    17221 tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata
    17281 ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc gacaggatat
    17341 tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc
    17401 aacagttccg ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc ctgatcatcg
    17461 ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc
    17521 atcgccttac acataatgta agaaaggata gcccccgagg aaccgaccag cgcaccggtc
    17581 acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac
    17641 gagttcagca tcgacaccac caccggcata tctgcaccac cgatggaggc gactaaatgc
    17701 cagccaaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg caggccgacg
    17761 ctatccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc tgccaggttc
    17821 attttgtgac ggtttggcag catcaacggt ttagacgaaa ttttgccaca cagtttgccg
    17881 aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag aaacacttcc
    17941 gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag atagctgtta
    18001 aagccaacca gcactgccgc cagacccacg aagctatgca ggatcgccac cagttctggc
    18061 atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc aatgaccatc
    18121 gccagcaaga tccagccaac gttacccgta tccggtccaa aaatggtggc gattagcgca
    18181 atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaagc
    18241 ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct
    18301 ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt
    18361 gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac
    18421 ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt
    18481 ggtgaccgac atcaacggtg tatgcagcgc gtgcgatacg ttccacacca cgtaataacc
    18541 gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct
    18601 tgccagccag ccgaaaagga tgattgccag cgccatcaac gcgtatttac gccacggtga
    18661 acaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgcca cctgcggctg
    18721 agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacagtcac
    18781 gccgcgaatc accacatcat caaaatcaac agtgatgtta ccgtttttct ctttgcacaa
    18841 cagtttcagc agattaacga ggttagtgcc gtaaagctgt gaggattgcg tcggcagacg
    18901 gcccggaaga tcggtatagc caatcacttt gacaccattt tccgtagtga agatttcacc
    18961 cggcacggtg tattcacagt tgccgccgtt ttgggctgcc aggtcgacaa tcacactgcc
    19021 cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat
    19081 aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc
    19141 cgctttgatg aacgcgtccg acatcacttt ggcatagcca tcgccgctgc ccgcttcctc
    19201 tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg
    19261 gcgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag
    19321 acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt
    19381 aatttgcccg gtaaagaagc acccaaattc atgtgccgct tcaacaatgg cgcgataacc
    19441 ggcgatattc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac
    19501 agagtccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt
    19561 ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg caatttcatc
    19621 atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac
    19681 aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc
    19741 gctctctacc gcgacggtaa accccagttt cagcagctgt tccactgttt ttggcgttgc
    19801 tgcaacacgg ggttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc
    19861 ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt
    19921 acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa
    19981 aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta
    20041 acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg
    20101 tcttcgacgg tcgggtaaaa cgagacatcg ccccggcacg aatcactact taacattaaa
    20161 ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc
    20221 atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg aaaaatgacg
    20281 cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca
    20341 gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc
    20401 gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga
    20461 gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat
    20521 tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg cctcttttta
    20581 tgttgtcgac acttctgatt ttgggaacag cggtaactgg cgtgtggtcg ctgacctcta
    20641 taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga
    20701 actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt
    20761 ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc
    20821 ctactctttc tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc agcgtattac
    20881 tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc
    20941 ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt
    21001 tgagatcccg ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gcttctttga
    21061 aacccagtca tcaaaaggcg ggcgttacac tgtcacgctt ccggatggca ctaaagtcga
    21121 agaactgaac aaagcgacag cagcgatgat ggtcccgttc gacagcatca aattctctgg
    21181 caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgagccg cgaagaaagg
    21241 tgctaaatat taccacatca cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag
    21301 tgcagatctg tataaataac tattcagtta ctcttataag gcggcttgat gccgcctttc
    21361 gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat
    21421 cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta
    21481 tcacgtttta atcactggat atcgatggaa aagaaactgg gactgagcgc actcaccgcg
    21541 ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca
    21601 gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg
    21661 ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg cggtatcttt
    21721 acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg
    21781 ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt
    21841 tttacggaca cgccggaatt gcgcctgttt ggcgatggta atacctggca atcgatagtc
    21901 ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct
    21961 gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg
    22021 ctggcgatga tgatgttcaa actggatatc ttcaagctcg acttcaccgg acttgcactt
    22081 ggcgtacctg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt
    22141 ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa
    22201 gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg
    22261 ctgggagtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg
    22321 atggtggaaa tgatgggacc gtggggcgaa atcatcatcg ctgccggttt gatcgtttcc
    22381 gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc
    22441 actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc
    22501 tcactgtggt tgacaaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt
    22561 tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg
    22621 gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt
    22681 gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt gctgttatcc
    22741 gttgttctgt atgcaccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat
    22801 gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt
    22861 ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata
    22921 acgatgggta aaacccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc
    22981 ctcgccctcg catggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggaca
    23041 cactatccag ccattgatgg actgattaat gcaggtgcgc agtgtattca ggctgatttt
    23101 tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagcac ccatggtctg
    23161 cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc gccactggcc
    23221 gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg
    23281 ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat
    23341 tatgtggtgg agcgcggtag cgacaaacat attgcgtatg ctgcaagcaa agcggcactg
    23401 gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt
    23461 gcgccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg
    23521 aataaatcat tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct ggtcgattac
    23581 ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg
    23641 cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgca
    23701 acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc
    23761 aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct
    23821 tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt
    23881 ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact
    23941 accagcgcac cgagcgcggc tttgattacc agccccatct ttttacctta acacttccat
    24001 aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga tagtaagtaa
    24061 aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga acactatcgt
    24121 atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg caaaacatga
    24181 tatacaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa
    24241 tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga ccatttgtcg
    24301 tgatttacgc gcaaagtggt ccggaccgat tgttcttcta acctctctcg atagcgatat
    24361 gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa cgacgccccc
    24421 tgctgttttg ttagcgcgtt tacgtttgca tttgcgtcag aatgagcaag ccacgctgac
    24481 caaaggtctt caggaaacgt cactgactcc ctacaaagcc ctgcatttcg gcacgttgac
    24541 catcgatcct atcaaccgcg tagtcaccct ggctaacact gaaatctcgc tctcgacagc
    24601 tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg accgcgatgc
    24661 attgctgaaa aatttacgcg gcgtcagtta tgacggcctg gatcgtagcg tggacgtggc
    24721 tatttcgcgg ttaagaaaaa aactgctcga taacgccgct gaaccttatc gcattaaaac
    24781 tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga tgaaaaaact
    24841 gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc tgctggttgg
    24901 gttggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg atttgatgaa
    24961 cagttcgctg tatctgatgc gcagcgaatt gcgtgagatc cccccacatg actggggtaa
    25021 gacgctgaaa gagatggatt taaatctctc tttcgatctg cgagtcgagc cactgagtaa
    25081 ataccatctt gatgatattt ccatgcaccg gctgcgtggc ggcgaaattg tcgccctgga
    25141 cgatcagtac acttttttgc agcgtatccc gcgcagccac tacgtgctgg cagttggtcc
    25201 tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc tgatcgcttt
    25261 tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact ggcaggatat
    25321 gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg aacgtatcca
    25381 ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga tggcggacaa
    25441 tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc acgaactgcg
    25501 aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga gcgccgccga
    25561 atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag agctgctgac
    25621 ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag acctgccgtt
    25681 gtggctgtca acgcatctgg cagatattca ggcagtaacg cccgataaaa cggtacggat
    25741 aaaaacgctc atgcaaggcc attatgcggc gttggatatg cgcttaatgg agcgcgtgct
    25801 ggataatttg ctcaataatg ccctgcgcta ctgccattca acggttgaaa ccagcctgct
    25861 actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga ttgccccaga
    25921 aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg atcgctcaac
    25981 cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa tgggcggtac
    26041 ggttaattgt gacaccagcg aactgggtgg tgcccgcttc tcgtttagct ggccgttatg
    26101 gcataacatc ccgcaattta cctctgcttg acactacgcg cacggtggtc aagtcaccac
    26161 cactgtgcta taaattaagg atgttgtaac taaagtggtt aatattatgg cgcgttacga
    26221 tctcgtagac cgactcaaca ctacttttcg ccagatggaa caagagctgg ctgcatttgc
    26281 cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc cggaggtaaa
    26341 aaaagaggat gagcataatc cgcttaatcg tattgaggtg aaacaacatc tcggcaacga
    26401 cgcgcagtcg caggcgttgc gtcatttccg tcatttattt attcaacaac agtccgaaaa
    26461 tcgcagcagc aaagcagctg tccgtctgcc tggcgtgttg tgttaccagg tcgataacct
    26521 ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga ccacgttcga
    26581 gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat gggtgcatcg
    26641 tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc tgcacgatcc
    26701 cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc atcgtgatga
    26761 agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac cgtggacgcg
    26821 cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc tgccacagaa
    26881 tgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg tctggtacaa
    26941 aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac tgattaatcg
    27001 ggataatggt gcgggcgtgc cggacgttgg tgagttgtta aattacgatg ccgacaatgt
    27061 gcagcaccgt tataaacctc aggcgcagcc gcttcgtttg atcattccac ggctgcacct
    27121 gtatgttgca gattaacgcc cggctttcat actgccgacc atctgttctg gccgtaccca
    27181 gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt ttaaggtcag
    27241 cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa tgtgggtgtt
    27301 aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct cacgattcgg
    27361 ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca gcaagcgcac
    27421 cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa agttaccgga
    27481 agcgcccccc atgttgatcg ccacgtcgtt ccccatcacc tgacagcaga gcatggttaa
    27541 tgcctcacac tgtgttgggt tcactttccc cggcatgatt gagctgcccg gctcattttc
    27601 cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc ggacatcatt
    27661 ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct gaaccagggc
    27721 atcacaggtc gccagcgctt caaatttgtt cggcgcggta acaaacggtg cacaggtaat
    27781 gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta gtccagtacc
    27841 caccgctgta ccgccaagag ccagttccgc tacgtgaggc aggctgtatt cgatatgttt
    27901 gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg ttagcggggt
    27961 ggcgtcctgc aagtgggttc gaccgatttt tacgatatcg gcaaatgcac gcgatttttc
    28021 actcagcgtc tgtgtcaggg ttttaagctg cggaatgagt tgcttgcgca gcgccagcag
    28081 cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt tgttcacgtc
    28141 gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt cactggcccg
    28201 gttagccagc acttcattca tgttcatgtt actttgcgtg ccggagccgg tctgccagat
    28261 agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg cctgccgaat
    28321 ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg ccgctgcgcg
    28381 cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg aaatgcggaa
    28441 atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga catcaatcgc
    28501 ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tactcctcac ctgattaatt
    28561 ttttctttct gttttgcttt cgttaagtaa cttttagctc actcattatt tactatttga
    28621 taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc ggctctgttt
    28681 atttcacaca gcgggtgcat tgtgtgagtt gtatctgctg gaagaagtca tttcctttat
    28741 catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg gcttccattc
    28801 ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc aatacagcgg
    28861 ccggaccacc gatactgcca aggtagaagc caccgtgttt tttacaggca tccgtcacct
    28921 gctggctgcg gttgcctttc gccagcatga tcatacttcc gcccagcgct tgcagttgat
    28981 cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag gcataacctt
    29041 ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttgatgtac tgcggcagcc
    29101 cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga ccgacgataa
    29161 tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt gcgaggatct
    29221 ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct ttgcgcagct
    29281 cttccgggat atatttgcct ggattatgtt ccagtttttc gatccagatc ccctgacggt
    29341 tgatcttcgc tttgatatta cggtcagcag aacaggagac gcccataccg accgggcagg
    29401 atgcgccgtg acgtggcagg cgaatcacgc ggatgtcgtg agcgaagtat ttaccgccaa
    29461 actgcgcacc cagaccaaga ttttgcgctt cgatcagcaa ttctttttcc aattccacat
    29521 cgcggaacgc ctgaccgtgc tcattccctt ccgttggcag ttcatcatag tatttcgcgg
    29581 aagccagttt caccgtttta aggttcgttt ctgcagaagt tccaccaata acgaacgcaa
    29641 tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc aggtaatttt
    29701 tcagtttccc cggcgtcagt aacgctttgg tttcctgata gagatacgtc ttgtttgccg
    29761 aaccaccacc tttggcgata cagaggaatt tgtattcgtc gccatcaacg gcataaagat
    29821 cgatctgcgc tggcagattg gtgccggtat tcacttcttt atacatatcc agcggcgcgt
    29881 tttgcgagta gcgcagatta tcttcgatat aagtgttata gacaccgcgc gccagcgccg
    29941 cttcatcacc accaccggtc catacacgct gccctttttt accaacaata atcgcggtgc
    30001 cggtatcctg acaggttggc agaacgcctt tcgccgcgat gtcggagtta cgcaggaatt
    30061 gcagcgccac atatttatca ttttcgctgg cctccgggtc acgcagaatg tcggccacct
    30121 gttgttggtg cgccggacgc agcatgaacg acgcatcatg aaacgcctgg cgcgccaaca
    30181 gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca gataagctaa
    30241 cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga aaaggagcct
    30301 gataatgaaa gggtttgttt gacattgttt tctcacttac tgcctggttt ggttatgctc
    30361 tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacgc acttttttaa
    30421 caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta ggattcttgc
    30481 tttaatagcg ggattaattt ccacattaaa acagggattg atcatgcaaa aactcattaa
    30541 ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt atggtatgga
    30601 aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga aaagcagttc
    30661 acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg agagtgataa
    30721 atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt tcctgttcaa
    30781 agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac acaagtctga
    30841 aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg agcgtaacta
    30901 taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc ttgcgatgaa
    30961 cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag gtgcacatcc
    31021 ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac tgttcgccag
    31081 cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa aatcggcgct
    31141 cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat tttacccgga
    31201 tgacagcggc ctgttctctc cgctgttgct gaatgtggtg aaattaaatc ctggcgaagc
    31261 gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc tggaagtgat
    31321 ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg atattccgga
    31381 actggttgcc aatgtgaagt tcgaagccaa accggctaac cagttgttga cccagccggt
    31441 gaaacaaggt gcagaactgg acttcccgat tcctgttgat gattttgcct tctcgctgca
    31501 tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt tgttctgcgt
    31561 cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac cgggtgaatc
    31621 agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc gtttagcacg
    31681 ggtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct aagcttatta
    31741 aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg ataatcatta
    31801 tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc tggacaggcg
    31861 gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc gcgcaggcga
    31921 acgcgcaact caaactgacc gctcctgaat ccaacctgga agtgagttat caaaactatc
    31981 atcgcggcgt attcagcagt cagctgcaac tgttggtgaa acccattgcc ggaaaagaaa
    32041 atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat catggtccct
    32101 tcccccttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca attcaaacca
    32161 cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt gaaacgcctt
    32221 ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt tcgctcaagc
    32281 cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag ttccagttaa
    32341 atgcggacag agacggcaaa gctatctccc tttccgggga ggcgcaaagt ggtcggatag
    32401 acgcggttaa cgaatacaac cagaaagtac agttgacctt taataatctg aaaaccgacg
    32461 gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg tcactggaaa
    32521 aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg gagatcagcg
    32581 gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat tactcgctaa
    32641 acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta aaagtcggcc
    32701 aaattgatgg cgaagcctgg catcagttta gccagcaata taacgcgcaa actcaggcgc
    32761 tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa gtgacggaag
    32821 ccttctttag cgccctgccg ctgatgttga aaggctatcc ggtgattact atcgcgccgc
    32881 taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc ctgaaagatc
    32941 cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt tcggttaaat
    33001 ctctggatgc gaaactgacc attccggtgg atatggcaac tgagttgatg actcaggtag
    33061 cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag caagttgaag
    33121 gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat accatcacca
    33181 ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg ccactggaag
    33241 atttcgttgg tatgtttgca atgccggcat taaatgttcc ggtcgtaccc gctattccgc
    33301 agcagtaatt cacagccaaa acgccggaga tttttctctc cggcgttatt ttttacttca
    33361 gcataaagtc atactgaata ctcattttca catcccagta attcaggcga tcgcccagcg
    33421 tactgccatc gcgcttttcc cgccctttat cggcagcaag aaagaaattc atcccttgta
    33481 acggcccttc gctgacccga tagttcatac cacctaagat acccgcgcct tcaatccgca
    33541 catctcgttt actggattca tagccgcgag tcacaatcgg tgcaaatgtc agtgtaaatt
    33601 gggagttaaa cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc
    33661 aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg
    33721 gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc
    33781 actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt cgagttgcgc
    33841 gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta tgcttttctc
    33901 gagaatactt tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg
    33961 actggtatca tcatgccaac tggcggctaa aaaagaacga gcgcgatgtt tatcgtattt
    34021 tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat cagtccataa
    34081 aaatactcgt gaacgccaca ttccccacag atcaatattt ggcgttaaag cccaataacc
    34141 taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat
    34201 acgataaggt aagctaccat aactataatc cgtcccgaag cgacctaatt taagcaggct
    34261 attttcactg ggttttattt ttactgcccc gagggcaaag ccaaaacttt tatcaccatc
    34321 aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat aatacgctcc
    34381 gccttcaacg ccaataatat tggaataata accactattg aaatcgagca atcctccctg
    34441 tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatcagccc tgcgcaattc
    34501 gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg cgtaagcaga
    34561 ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt
    34621 tgcacctttg atgttctg
//
LOCUS       DAZGXS010000036        33651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGXS010000036 DAZGXS010000000
VERSION     DAZGXS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            311..1249
                     /gene="tdcA"
                     /locus_tag="ACHKG5_001894"
     CDS             311..1249
                     /gene="tdcA"
                     /locus_tag="ACHKG5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0868005.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1348..2337
                     /gene="tdcB"
                     /locus_tag="ACHKG5_001895"
     CDS             1348..2337
                     /gene="tdcB"
                     /locus_tag="ACHKG5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP0868006.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2359..3690
                     /gene="tdcC"
                     /locus_tag="ACHKG5_001896"
     CDS             2359..3690
                     /gene="tdcC"
                     /locus_tag="ACHKG5_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0868007.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3716..4924
                     /gene="tdcD"
                     /locus_tag="ACHKG5_001897"
     CDS             3716..4924
                     /gene="tdcD"
                     /locus_tag="ACHKG5_001897"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0868008.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4958..7252
                     /gene="tdcE"
                     /locus_tag="ACHKG5_001898"
     CDS             4958..7252
                     /gene="tdcE"
                     /locus_tag="ACHKG5_001898"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0868009.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7266..7655
                     /gene="tdcF"
                     /locus_tag="ACHKG5_001899"
     CDS             7266..7655
                     /gene="tdcF"
                     /locus_tag="ACHKG5_001899"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0868010.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7727..9091
                     /gene="tdcG"
                     /locus_tag="ACHKG5_001900"
     CDS             7727..9091
                     /gene="tdcG"
                     /locus_tag="ACHKG5_001900"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0868011.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9366..10697
                     /gene="cyuP"
                     /locus_tag="ACHKG5_001901"
     CDS             9366..10697
                     /gene="cyuP"
                     /locus_tag="ACHKG5_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0868012.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            10725..12035
                     /gene="cyuA"
                     /locus_tag="ACHKG5_001902"
     CDS             10725..12035
                     /gene="cyuA"
                     /locus_tag="ACHKG5_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0868013.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12169..12333)
                     /gene="yhaL"
                     /locus_tag="ACHKG5_001903"
     CDS             complement(12169..12333)
                     /gene="yhaL"
                     /locus_tag="ACHKG5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0868014.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12356..13057)
                     /gene="yhaK"
                     /locus_tag="ACHKG5_001904"
     CDS             complement(12356..13057)
                     /gene="yhaK"
                     /locus_tag="ACHKG5_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0868015.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13162..14058
                     /gene="yhaJ"
                     /locus_tag="ACHKG5_001905"
     CDS             13162..14058
                     /gene="yhaJ"
                     /locus_tag="ACHKG5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0868016.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            complement(14109..14465)
                     /gene="yhaI"
                     /locus_tag="ACHKG5_001906"
     CDS             complement(14109..14465)
                     /gene="yhaI"
                     /locus_tag="ACHKG5_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0868017.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(14706..15071)
                     /gene="yhaH"
                     /locus_tag="ACHKG5_001907"
     CDS             complement(14706..15071)
                     /gene="yhaH"
                     /locus_tag="ACHKG5_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0868018.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(15363..16349)
                     /gene="yqjG"
                     /locus_tag="ACHKG5_001908"
     CDS             complement(15363..16349)
                     /gene="yqjG"
                     /locus_tag="ACHKG5_001908"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0868019.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(16419..16811)
                     /gene="yqjF"
                     /locus_tag="ACHKG5_001909"
     CDS             complement(16419..16811)
                     /gene="yqjF"
                     /locus_tag="ACHKG5_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0868020.1"
                     /translation="MKKLEDVCVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(16997..17296)
                     /gene="yqjK"
                     /locus_tag="ACHKG5_001910"
     CDS             complement(16997..17296)
                     /gene="yqjK"
                     /locus_tag="ACHKG5_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0868021.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17286..17690)
                     /gene="yqjE"
                     /locus_tag="ACHKG5_001911"
     CDS             complement(17286..17690)
                     /gene="yqjE"
                     /locus_tag="ACHKG5_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0868022.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(17693..17998)
                     /gene="yqjD"
                     /locus_tag="ACHKG5_001912"
     CDS             complement(17693..17998)
                     /gene="yqjD"
                     /locus_tag="ACHKG5_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0868023.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(18036..18404)
                     /gene="yqjC"
                     /locus_tag="ACHKG5_001913"
     CDS             complement(18036..18404)
                     /gene="yqjC"
                     /locus_tag="ACHKG5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0868024.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(18551..18934)
                     /gene="mzrA"
                     /locus_tag="ACHKG5_001914"
     CDS             complement(18551..18934)
                     /gene="mzrA"
                     /locus_tag="ACHKG5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0868025.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(18938..19600)
                     /gene="yqjA"
                     /locus_tag="ACHKG5_001915"
     CDS             complement(18938..19600)
                     /gene="yqjA"
                     /locus_tag="ACHKG5_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0868026.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(19622..19705)
                     /gene="yqiM"
                     /locus_tag="ACHKG5_001916"
     CDS             complement(19622..19705)
                     /gene="yqiM"
                     /locus_tag="ACHKG5_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP0868027.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(19945..20721)
                     /gene="exuR"
                     /locus_tag="ACHKG5_001917"
     CDS             complement(19945..20721)
                     /gene="exuR"
                     /locus_tag="ACHKG5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0868028.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(20851..22149)
                     /gene="exuT"
                     /locus_tag="ACHKG5_001918"
     CDS             complement(20851..22149)
                     /gene="exuT"
                     /locus_tag="ACHKG5_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0868029.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            22632..24044
                     /gene="uxaC"
                     /locus_tag="ACHKG5_001919"
     CDS             22632..24044
                     /gene="uxaC"
                     /locus_tag="ACHKG5_001919"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0868030.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            24059..25546
                     /gene="uxaA"
                     /locus_tag="ACHKG5_001920"
     CDS             24059..25546
                     /gene="uxaA"
                     /locus_tag="ACHKG5_001920"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0868031.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            25629..26180
                     /gene="ygjV"
                     /locus_tag="ACHKG5_001921"
     CDS             25629..26180
                     /gene="ygjV"
                     /locus_tag="ACHKG5_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0868032.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(26185..27429)
                     /gene="sstT"
                     /locus_tag="ACHKG5_001922"
     CDS             complement(26185..27429)
                     /gene="sstT"
                     /locus_tag="ACHKG5_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0868033.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(27828..28793)
                     /gene="alx"
                     /locus_tag="ACHKG5_001923"
     CDS             complement(27828..28793)
                     /gene="alx"
                     /locus_tag="ACHKG5_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0868034.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(29077..30063)
                     /gene="ygjR"
                     /locus_tag="ACHKG5_001924"
     CDS             complement(29077..30063)
                     /gene="ygjR"
                     /locus_tag="ACHKG5_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0868035.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(30142..30834)
                     /gene="ygjQ"
                     /locus_tag="ACHKG5_001925"
     CDS             complement(30142..30834)
                     /gene="ygjQ"
                     /locus_tag="ACHKG5_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0868036.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(30911..31414)
                     /gene="ygjP"
                     /locus_tag="ACHKG5_001926"
     CDS             complement(30911..31414)
                     /gene="ygjP"
                     /locus_tag="ACHKG5_001926"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0868037.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            31499..32635
                     /gene="rlmG"
                     /locus_tag="ACHKG5_001927"
     CDS             31499..32635
                     /gene="rlmG"
                     /locus_tag="ACHKG5_001927"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0868038.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            32919..33233
                     /gene="higB"
                     /locus_tag="ACHKG5_001928"
     CDS             32919..33233
                     /gene="higB"
                     /locus_tag="ACHKG5_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFP0868039.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            33230..33646
                     /gene="higA"
                     /locus_tag="ACHKG5_001929"
     CDS             33230..33646
                     /gene="higA"
                     /locus_tag="ACHKG5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0868040.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         8401 a         8309 c         8707 g         8234 t
ORIGIN      
        1 tttaactcaa attttgcctg gtaattatcc ggtaattgct tgaaatatgg tctcagcccc
       61 ttttttgtat taaccacata acgaatgatg ttatcgccat aaaatatggt tatccccgtc
      121 attttttttg acaaaaatca gggtttatgc tgatttttat actttaattt attgatattt
      181 aaaggtattt aattgacatg acgataccct ggaaagtatt gaaagttaat ttgtgagtgg
      241 tcgcacatat cctgttcatt tcattttgat acacttcatg ccgtcaatga ggtaattaac
      301 gtaggtcgtt atgagcacta ttcttcttcc gaaaacgcag cacctggtag tctttcagga
      361 agtcattaga agtggttcta tcggctcggc tgcaaaagaa ttagggttaa ctcaaccggc
      421 cgtcagtaaa atcattaacg atattgaaga ttattttggt gtggaattag tggtgcggaa
      481 aaataccgga gtaacattaa cacctgccgg tcaattgtta ctctctcgtt ccgaatccat
      541 tacccgtgaa atgaaaaata tggttaatga gataagcggt atgtcttctg aggcggtggt
      601 ggaagtctca tttggttttc cttcattgat tggttttact tttatgtcag ggatgatcaa
      661 caagttcaaa gaggtgttcc cgaaagcgca ggtttctatg tatgaagcgc aactgtcttc
      721 gttcttaccg gcaatccgcg acggtcgact tgattttgcg attggtacgt taagcgcaga
      781 aatgaagctt caggatttac atgttgagcc gctgttcgag tccgagtttg tgctggtagc
      841 cagtaagtcc cgaacatgca ccggcaccac cacgctggag tcgttgaaga acgaacagtg
      901 ggtgttgcca caaactaata tggggtacta cagcgaactg cttactacgt tacaaagaaa
      961 tggcatcagt attgaaaaca tcgttaaaac cgactcagtc gtgacaattt ataatcttgt
     1021 tctcaatgct gatttcttaa ctgtaattcc ttgtgatatg acgtctcctt ttggttctaa
     1081 tcaatttatt actattccgg ttgaagaaac attacctgtg gcacaatatg ccgcggtatg
     1141 gtcgaaaaat tatcgtatta aaaaagcagc atcggttttg gtggaattag ccaaagagta
     1201 ttcatcttat aatgggtgta gacgaaggca attaatagaa gttggttagt tatttgtttt
     1261 tatttaaaca taaataatcc acctgtctgt cttgcaggtg tcggttacgg ttacctacat
     1321 atttaattca ggcgaagagg ttttataatg catattacat acgatctgcc ggttgctatt
     1381 gatgacatta ttgaagcgaa acaacgactg gctgggcgaa tttataaaac aggcatgcct
     1441 cgctccaact attttagtga acgttgcaaa ggtgaaatat tcctgaagtt tgaaaatatg
     1501 cagcgtacgg gttcatttaa aattcgtggc gcatttaata aattaagttc actgactgat
     1561 gcggaaaaac gcaaaggcgt ggtggcctgt tctgcgggca accatgcgca aggggtttcg
     1621 ctctcctgcg cgatgctggg tatcgacggt aaagtggtga tgccaaaagg cgcgccgaaa
     1681 tccaaagtag cggcaacgtg cgactactcc gcagaagtcg ttctgcatgg tgataacttc
     1741 aacgacacta tcgctaaagt gagcgaaatt gtcgaaatgg aaggccgtat ttttatccca
     1801 ccttacgatg atccgaaagt gattgctggt cagggaacga ttggcctgga aattatggaa
     1861 gatctctatg atgtcgataa cgtgattgtg ccaattggtg gtggcggttt aattgctggt
     1921 attgcggtgg caattaaatc tattaacccg accattcgtg ttattggcgt acagtctgaa
     1981 aacgttcacg gcatggcggc ttctttccac tccggagaaa taaccacgca ccgaactacc
     2041 ggcaccctgg cggatggttg tgatgtctcc cgcccgggta atttaactta cgaaatcgtt
     2101 cgtgaattag tcgatgacat cgtgctggtc agcgaagatg aaatcagaaa cagtatgatt
     2161 gccttaattc agcgcaataa agtcgtcacc gaaggcgcag gcgctctggc atgtgctgca
     2221 ttattaagcg gtaaattaga ccaatatatt caaaacagaa aaaccgtcag tattatttcc
     2281 ggcggcaata tcgatctttc tcgcgtctct caaatcaccg gtttcgttga cgcttaatta
     2341 attcgttgag gataggatat gagtacttca gatagcattg tatccagcca gacaaaacaa
     2401 tcgtcctggc gtaaatcaga taccacatgg acgttaggct tgtttggtac ggcaatcggc
     2461 gccggggtgc tgttcttccc tatccgcgca ggttttggcg gactgatccc gattcttctg
     2521 atgttggtat tggcataccc catcgcattt tattgccacc gggcgctggc gcgtctgtgt
     2581 ctttctggct ctaacccttc cggcaacatt acggaaacgg tggaagagca ttttggtaaa
     2641 actggcggcg tggttatcac gttcctgtac ttcttcgcga tttgcccact gctgtggatt
     2701 tatggcgtta ctattaccaa tacctttatg acgttctggg aaaaccagct cggctttgca
     2761 ccgctgaatc gcggctttgt ggcgctgttc ctgttgctgc tgatggcttt cgtcatctgg
     2821 tttggtaagg atctgatggt taaagtgatg agctacctgg tatggccgtt tatcgccagc
     2881 ctggtgctga tttctttgtc gctgatccct tactggaact ctgcagttat cgaccaggtt
     2941 gacctcggtt cgctgtcgtt aaccggtcat gacggtatcc tgatcactgt ctggctgggg
     3001 atttccatca tggttttctc ctttaacttc tcgccaatcg tctcttcctt cgtggtttct
     3061 aaacgtgaag agtatgagaa agacttcggt cgcgacttca ccgaacgtaa atgttcccaa
     3121 atcatttctc gtgccagcat gctgatggtt gcagtggtga tgttctttgc ctttagctgc
     3181 ctgtttacgc tgtctccggc caacatggcg gaagccaaag cgcagaatat tccagtgctt
     3241 tcttatctgg ctaaccactt tgcgtccatg accggtacca aaacaacgtt cgcgattaca
     3301 ctggaatatg cggcttccat catcgcactc gtggctatct tcaaatcttt cttcggtcac
     3361 tatctgggaa cgctggaagg tctgaatggc ctggtcctga agtttggtta taaaggcgac
     3421 aaaactaaag tatcgctggg taaactgaac actatcagca tgatcttcat catgggctcc
     3481 acctgggttg ttgcctacgc caacccgaac atcctcgacc tgattgaagc catgggcgca
     3541 ccgattatcg catccctgct gtgcctgttg ccgatgtatg ccatccgtaa agcgccgtct
     3601 ctggcgaaat accgtggtcg tctggataac gtgtttgtta ccgtgattgg tctgctgacc
     3661 atcctgaaca tcgtatataa actgttttaa tccgtaactc aggatgagaa aagagatgaa
     3721 tgaatttccg gttgttttgg ttattaactg tggttcgtct tcgattaagt tttccgtact
     3781 cgatgccagc gactgtgaag tattaatgtc aggtattgcc gacggtatta actcggaaaa
     3841 tgcattctta tccgtaaatg ggggagagcc agcaccgctg gctcaccaca gctacgaagg
     3901 tgcattgaag gcaattgcat ttgaactgga aaaacggaat ttaaatgaca gcgtggcctt
     3961 aattggccac cgcatcgctc acggcggcag tatttttacc gagtccgcca ttattaccga
     4021 tgaagtcatt gataatatcc gtcgcgtttc tccactggca cccctgcata attacgccaa
     4081 tttaagtggt attgaatcgg cgcagcaatt atttccgggc gtaactcagg tggcggtatt
     4141 tgataccagt ttccaccaga cgatggctcc ggaagcttat ttatacggcc tgccgtggaa
     4201 atattatgaa gagttaggtg tacgccgtta tggtttccac ggcacgtcgc accgctatgt
     4261 ttccctgcgc gcacattctt tgctgaatct ggcggaagat gactccggcc tggttgtggc
     4321 gcatcttggc aatggcgcgt caatctgcgc agttcgcaac ggtcagagtg ttgacacctc
     4381 aatgggaatg acgccgctgg aaggcttgat gatgggtacc cgcagtggcg atgtcgactt
     4441 tggtgcgatg tcctgggtcg ccagccaaac caaccagagc ctgggtgacc tggaacgcgt
     4501 agtgaataaa gagtcgggat tattaggtat ttccggtctt tcttcggatt tacgtgttct
     4561 ggaaaaagcc tggcatgaag gtcacgaacg cgcgcagctg gcaattaaaa cctttgttca
     4621 ccgaattgcc cgtcatattg ccggacacgc agcttcatta cgtcgcctgg atggaattat
     4681 attcaccggc ggaataggag agaattcaag tttaattcgt cgtctggtca tggaacattt
     4741 ggctgtatta ggcgtagaga ttgatacaga aatgaataat cgatctaact cttttggcga
     4801 gcgaattgtt tccagtgaaa atgcgcgtgt catttgcgcc gtaattccga ccaacgaaga
     4861 aaaaatgatt gctttggatg ccattcattt aggcaaagtt aacgcacccg cagaatttgc
     4921 ataatttagt tgaagtattg tagagagatt atttttcatg aaggtagata ttgataccag
     4981 cgataagctg tacgccgacg catggcttgg ctttaaaggt acggactgga aaaacgaaat
     5041 taatgtccgc gattttattc aacataacta tacaccgtat gaaggcgatg aatctttcct
     5101 cgctgaagcg acgccagcca ccacggaatt gtgggaaaag gtgatggaag gcattcgtat
     5161 tgaaaacgca acccacgcgc cggttgattt cgataccaat attgccacca caattaccgc
     5221 tcatgatgcg ggatatatta accagccgct ggaaaaaatt gttggcctgc aaacggatgc
     5281 gccgttgaaa cgtgcgttac acccgttcgg tggcattaat atgattaaaa gttcattcca
     5341 cgcctatggt cgtgaaatgg acagtgaatt tgaatatctg tttaccgatc tgcgtaaaac
     5401 ccataaccag ggcgtatttg atgtttactc accggatatg ctgcgctgcc gtaaatctgg
     5461 cgtgctgacc ggtttaccag atggctatgg ccgtgggcgc attatcggtg actatcgccg
     5521 cgtagcgctg tatggcatca gttatctggt gcgtgaacgc gaactgcaat ttgccgatct
     5581 ccagtctcgt ctggaaaaag gcgaggatct ggaagccacc atccgtctgc gtgaggagct
     5641 ggcagagcat cgtcatgcgc tgttgcagat tcaggaaatg gcggcgaaat atggctttga
     5701 tatctctcgc ccggcgcaga atgcgcagga agcggtgcag tggctctact tcgcttatct
     5761 ggcggcagtg aaatcacaga acggcggcgc gatgtcgctg ggccgcacgg catcgttcct
     5821 ggatatctac attgagcgcg actttaaagc tggcgtactc aatgagcagc aggcacagga
     5881 actgatcgat cacttcatca tgaagatccg tatggtgcgc ttcttgcgta cgccggaatt
     5941 tgattcgctg ttctccggcg acccaatctg ggcgacggaa gtgatcggcg ggatggggct
     6001 ggacggtcgt acgctggtga ccaaaaactc cttccgctat ttgcacaccc tgcacactat
     6061 ggggcctgca ccggaaccta acctgaccat tctttggtcg gaagaattac cgattgcctt
     6121 caaaaaatat gccgcgcagg tgtcgatcgt cacctcttcc ttgcagtatg aaaacgacga
     6181 tctgatgcgt actgacttca acagcgacga ttacgcgatt gcctgctgcg tcagcccaat
     6241 ggtgattggt aagcaaatgc agttctttgg tgcacgcgct aacctggcga aaacactgct
     6301 gtacgcaatt aacggcgggg tggacgagaa gctgaagatt caggtcgggc cgaaaacggc
     6361 accgttgatg gacgatgtgc tggattacga caaagtgatg gacagcctcg atcacttcat
     6421 ggactggctg gcggtgcagt acatcagcgc gctgaatatc attcactaca tgcacgacaa
     6481 gtacagctac gaagcttcgc tgatggcgct gcacgatcgt gatgtctatc gcaccatggc
     6541 gtgcggcatc gcgggcctgt cggtggcaac ggactccctg tctgcgatca aatatgcccg
     6601 cgtgaaacca atccgtgaca aaaacggcct ggcggtggac tttgaaatcg acggtgaata
     6661 tccgcagtac ggcaacaacg acgagcgcgt agacagcatt gcctgcgacc tggttgaacg
     6721 ttttatgaag aaaattaaag cgctgccaac ctatcgcaac gccgtcccta cccagtcgat
     6781 tctgactatc acttctaacg tggtgtacgg ccagaaaacc ggtaacacgc cggacggtcg
     6841 tcgcgccgga acaccgttcg cgccgggcgc taacccgatg catggtcgtg accgcaaagg
     6901 tgccgtggcc tcattgacgt cggtggcgaa actgccgttc acctacgcca aagatgggat
     6961 ctcgtacacc ttctcaatcg tccctgcggc gctgggcaaa gaagatccag tacgtaaaac
     7021 caaccttgtc ggcctgctgg atgggtattt ccaccacgaa gcggatgtcg aaggcggtca
     7081 acacctcaac gtcaacgtaa tgaatcggga aatgctgctg gatgccatcg agcacccgga
     7141 aaaatatcct aacctgacaa tccgtgtctc tggctacgcc gtgcgcttca acgcactgac
     7201 ccgtgaacag cagcaggatg ttatttcacg tacctttacc caggcgctct gacgccggag
     7261 gatgtatgaa aaagattatc gaaacgcaac gtgccccagg cgcaatcggc ccttatgttc
     7321 aaggcgttga tttaggcagc atggtcttca cctccggtca aattccggtt tgcccacaga
     7381 ccggtgagat cccggctgat gtgcaagatc aggcgcgttt aagcctcgaa aacgtcaaag
     7441 cgatcgtggt tgctgccggg ctgagcgtgg gcgatatcat caagatgacc gtgttcatca
     7501 ccgatctgaa tgattttgcc accatcaacg aagtctataa gcaattcttc gatgagcatc
     7561 aggcgaccta cccgacccgg agctgtgtac aggtcgcgcg tttgccgaaa gatgtgaagc
     7621 tggaaattga agctatcgca gtacgtagtg cgtaaagcct cgtgagcggg acggtcgtaa
     7681 ggtcgttccg ctccacttca ctgaacggca atccgagggt gtggatatga ttagtgcatt
     7741 cgatattttc aaaattggga ttggtccctc cagttcgcat accgtggggc caatgaatgc
     7801 cggaaaaagt tttattgatc ggctggaaag tagcggctta ttaaccgcga cgagccatat
     7861 tgtggtcgat ctgtacgggt cgttgtcact gacgggcaaa ggccatgcca cggatgtcgc
     7921 catcatcatg ggactggcag gaaacagtcc gcaggatgtt gtcattgatg agatccctgc
     7981 atttatagag ttagtaacgc gcagcgggcg gctgccagtg gcatctggtg cgcatattgt
     8041 tgattttcct gtagcaaaga acattatctt ccatcccgaa atgttgcctc gccatgagaa
     8101 cggaatgcgg atcactgcct ggaaggcaca ggaagagcta ttaagtaaaa cctattactc
     8161 tgtcggcggc gggtttattg tcgaagaaga acacttcggc ctgtcgcacg atgtcgaaac
     8221 acccgtacct tacgatttcc actcagcagg tgaactgctg aaaatgtgtg attacaacgg
     8281 cctgtctatt tctggcctga tgatgcacaa cgagctagcg ctgcgcagta aagcggaaat
     8341 tgacgccggt tttgcccgta tctggcaagt gatgcatgac ggtattgaac gcgggatgaa
     8401 cactgaaggc gtgctgcctg gtccgctcaa tgtgccgcgc cgtgccgtag cgctgcgtcg
     8461 tcagctggtt tccagcgata acatctctaa cgatccgatg aatgtcatcg actggatcaa
     8521 catgtacgcg ctggcggtta gtgaagaaaa cgcagctggc gggcgcgtgg taacggcacc
     8581 gactaacggt gcgtgcggca ttattccggc agtgctggct tattacgata agttccgtcg
     8641 tccggtaaac gagcggtcaa ttgcccgcta ttttcttgca gccggggcta ttggcgcgct
     8701 gtataaaatg aacgcctcca tctctggcgc ggaggtcggc tgtcaggggg agattggcgt
     8761 ggcctgttca atggcggcgg cagggttaac tgaactactg ggcggcagtc cggcgcaggt
     8821 atgcaatgcg gcggaaatcg cgatggagca taaccttgga ctgacctgcg atccggttgc
     8881 cggacaggta caaatcccgt gcattgaacg taatgccatt aatgccgtga aagcagtaaa
     8941 cgccgcgcgg atggcgatgc gccgcacctc ggcaccgcgt gtttcactcg ataaagtgat
     9001 cgagacgatg tatgaaaccg gcaaagatat gaacgataaa taccgcgaaa catcacgcgg
     9061 cggactggcc attaaagtgg tctgcggctg acattgctgt cagctttcat ccttgggtgc
     9121 acaaatgtgc accttttttt tgtgatctgc cgcaaataga gagtgaatat tctcttgcga
     9181 ttaatttagt agaaaattct tatccatctc cgttatttca aagtcttgat tgtgaagatt
     9241 ttctctgacc tcgcgcgtat catttcttta ttctaataac tcgcctgcaa aaacgtagac
     9301 cgcatatcgc cttgatgcgg ctcggaaaaa aataataagc cctacaaaat attgagcgag
     9361 aaattatgga aattgcatcg aataaaggcg tcattgcaga cgcttcgacc ccggcgggtc
     9421 gtgctggaat gagtgagagc gagtggcgag aagcgatcaa attcgacagt actgacaccg
     9481 gctgggtgat tatgagtatc gggatggcga ttggcgcggg gattgttttt ctcccggtgc
     9541 aggtcggttt gatgggattg tgggtatttt tgctctcatc ggtgattggt tacccggcaa
     9601 tgtatctgtt tcagcggttg tttattaata cgctggcaga atcaccagaa tgtaaagatt
     9661 acccgagcgt cattagcggt tatttaggta aaaactgggg catcctgtta ggtgcactct
     9721 atttcgtaat gctggtgatt tggatgttcg tctattccac cgccatcacc aacgatagtg
     9781 cttcctacct gcataccttc ggcgtgacgg aagggttgct gtcagacagt cccttttatg
     9841 gtctggtact gatttgcatt ctggtggcga tctcttcacg cggcgagaaa ttgttattca
     9901 aaatttcgac tggcatggtg ctgaccaagc tgctggtggt cgcggcgctg ggcgtgtcga
     9961 tggtgggaat gtggcatctg tataacgtcg gttcgctacc gccgctgggg ctgctggtga
    10021 aaaacgctat tattacgctg ccgtttaccc tgacgtcgat tctgtttatc cagacgttaa
    10081 gcccgatggt gatctcttat cgctcgcggg aaaaatcgat tgaagtagcg cggcataaag
    10141 cattgcgggc aatgaatatc gcgtttggca ttttgtttat catcgtcttt ttctacgccg
    10201 tgtcgttcac gctggcgatg ggacatgacg aagcggtaaa agcctacgag cagaatattt
    10261 ccgcgctggc gattgccgca cagtttatta gcggtgacgg cgcagcgtgg gtgaaagtgg
    10321 tcagcgtcat tctcaatatc tttgctgtca tgactgcatt ctttggcgtc tatttaggtt
    10381 ttcgcgaagc aacgcaaggg atcgtaatga acatcctgcg tcgcaagatg cctgccgaga
    10441 agattaacga aaatcttgtt cagcgcggca tcatgatttt cgccattttg ctggcctgga
    10501 gcgccatcgt actgaacgca ccggtgttga gcttcacctc tatctgtagc ccgattttcg
    10561 gcttggtagg gtgcctgatc ccggcgtggc tggtttacaa agtaccggca ttgcacaaat
    10621 acaaagggat gtctctgtac ctgattatcg tcactggttt gttgctttgt gtttctccgt
    10681 tcctggcatt ttcttgattt acctgaaatt ttaaggtttt taatatgttt gattcgactt
    10741 taaatccgtt atggcagcgt tacatcctcg ccgttcagga ggaagtaaaa ccggcgctgg
    10801 gatgtactga accgatttca ctggcgctgg cggcggcggt tgctgcggca gaactggaag
    10861 gtccggttga acgtgtagaa gcctgggttt cgccaaatct gatgaagaac ggtctgggcg
    10921 tcaccgttcc cggcacggga atggtggggc tgccgattgc ggcggcgctg ggggcgttag
    10981 gtggaaatgc caacgccggg ctggaagtgc tgaaagacgc aactgcgcag gcaattgccg
    11041 atgccaaagc actgctggcg gcggggaaag tctccgttaa gatccaggaa ccttgcaatg
    11101 aaatcctctt ctcacgcgcc aaagtctgga acggtgagaa gtgggcgtgt gtcactatcg
    11161 tcggcgggca taccaacatt gtgcatattg agacgcacga tggtgtggtg tttacccagc
    11221 aggcgtgtgt ggcagagggc gagcaagagt ctccgctatc ggtgctttcc agaacgacgc
    11281 tggctgagat cctgaagttc gtcaatgaag tcccgtttgc ggcgatccgc tttattctcg
    11341 attccgcgaa gctaaattgt gcgttatcgc aggaaggttt gagcggtaag tgggggctgc
    11401 atattggcgc gacgctggaa aaacagtgcg agcgcggttt gctggcgaaa gatctctctt
    11461 catccattgt gattcgtacc agcgcggcat ccgatgcgcg tatgggcggc gccacgcttc
    11521 cggcaatgag taactccggc tcgggtaacc aggggatcac cgcaacaatg cccgtggtgg
    11581 tggtagcaga acacttcgga gcggatgatg aacggctggc gcgtgcgctg atgctttctc
    11641 atttgagcgc aatttacatc cataaccagt taccgcgttt gtctgcgctg tgtgccgcaa
    11701 cgaccgcagc aatgggggcc gccgccggga tggcatggct ggtggatggg cgttatgaaa
    11761 ccatctcgat ggcgatcagc agtatgatcg gcgatgtcag cggcatgatt tgcgatggtg
    11821 cgtcgaacag ctgcgcgatg aaggtttcga ccagtgcttc ggctgcgtgg aaagcggtgt
    11881 taatggcgct ggatgatacc gccgtgaccg gcaatgaagg gatcgtggcg catgatgttg
    11941 agcagtcgat tgccaacctg tgtgcgttag caagccattc gatgcagcaa acggatcggc
    12001 agattatcga gattatggcg agcaaggcca gataaacgcc aggttttggg tatcgtgccg
    12061 gatgcgatgc tggcgcatct tatccggcct acgggttgtt taattcgtta atgttgttgg
    12121 attttgtagg ccggataagg cgttttcgcc gcatccggca acctgtcttt acgcggtagc
    12181 ttcatcctcg gctaaacgcg tttcagcatc cgcgacgatg gcttccagct cgctcaacat
    12241 ctcttcatag ccaaaatttt tggcggtgcg agcaggttct ttcgccacca cgggcttcac
    12301 tggctggcgc tttttggccg attttttact catcatcatc tccctgtaaa aacaactaga
    12361 caggcaaatc tatcagcaaa gcgcgcagtg gggaatcggc aaccagcgta atgttagcct
    12421 cgtcacgaat aaacgcccca tcaccgcagg tcagcgccgc tttctcttca tgatgcgtaa
    12481 gcgcatgaaa tttcccgtga atcgattgca aatacgtgcg tggcccatgc aactggaaat
    12541 tcgcactttc gcctttgtcg agcacgatat ggtgcagcca cacttgctgg cgtaattgca
    12601 ggcttcccat cgcaccctct ggcgaggcga ttaaatgctg cttgcccata ttaagcgcca
    12661 gtttttgaat cagcggattc tctcgctgcg ggcaggcgtc cagccaaagc tgcattcgtg
    12721 ttaacggttt gtctttgctg agattgtgtt cgctgtagct gacacccggc tgggtagaga
    12781 gcagcaacgc ctcaccggcg ctggcctgaa catgattgcc ttcgctgtcg cgatactctg
    12841 cttctccatc cagaatcaca tttaaaatat cgactttggg ataggttcgc ggctgaaagg
    12901 cggcacctgg ggccagcact tcctggttaa gcacacgcag ggaggcatag cctaacaatt
    12961 tcgggtcgaa gtagtgtcca aaggaaaaag tataccgggc ctgcaaccat ccgtagtctg
    13021 cttgtccaca ctgcctggca gttcgggtag taatcataat tcttgacctc tctttacttc
    13081 cttttatggt aaggggctgg acgctacatt gttagccaga tattctgccc ggtatgttca
    13141 aatttcctga atgagaacga aatggccaaa gaaagggcat taacgctgga agcactacgg
    13201 gttatggatg cgatcgatcg ccggggcagt tttgcggcgg cagcggatga gctgggacgc
    13261 gtgccttccg cacttagcta caccatgcaa aaactggaag aagagctgga tgtggtgctg
    13321 tttgaccgct cgggccatcg caccaaattc accaatgtcg ggaggatgtt gttggagcga
    13381 gggcgcgttt tgctggaagc cgcagataaa ctgactactg atgcggaagc tctcgcgcgc
    13441 ggttgggaaa cgcatctcac cattgtgacc gaagcgctgg taccgacacc tgcctttttc
    13501 ccgttaatcg acaaactggc ggcaaaagcc aatacccaac tggcaatcat cacagaagtg
    13561 ctggcggggg cgtgggaacg gctggagcag gggcgggcgg atattgttat cgcgccggat
    13621 atgcattttc gttcctcgtc ggagatcaac tcgcgcaagc tctatacgtt aatgaacgtc
    13681 tacgttgccg cgcctgatca cccgattcat caggagccgg aaccgttatc tgaagtgacg
    13741 cgtgtgaaat atcgtggaat tgcggtggcg gataccgctc gtgagcgccc ggtgttgact
    13801 gtacagctgc tggacaaaca gccgcgctta acggtgagca cgattgaaga taaacgtcag
    13861 gcattactgg cggggcttgg cgtggcgacg atgccgtatc cgatggtcga aaaagatatt
    13921 gcggaagggc ggttgcgtgt cgtcagcccg gaatcgacca gcgagatcga tattattatg
    13981 gcctggcgtc gtgacagtat gggggaagcg aaatcctggt gtctgcggga aattcccaaa
    14041 ctttttaacg gaaaataata tttattattg ttaataaaaa ggggcgaaat atcgccccaa
    14101 ttctaagatt aatttgaacc aaacttcgga tcgtttccgt aacgattgct gccagaagta
    14161 ccttctgtgc agaaaaaaac cagaagcacg agccagccaa taaacggaac aaaaaagagc
    14221 aaggcccagg cacctgaacg gtcggtatcg tgtaagcgac ggatagcaag cgcaaggacg
    14281 ggaaggaagg ttgccagcaa ataaagcata gacagatatg gaagctccag acctaaaatc
    14341 aattgaatga cattgataat agcgccgacg atggcgttaa tcagagtaaa catccagtac
    14401 tctttacgac gtgcgcgacc agagaaacca acataatttt ttaatacgga taaataccac
    14461 tgcataaaaa catcccttta attaaaagtg acgaaagtct attccgggta ttgtgtaaat
    14521 gctattcgta tctttctttt tcggatgtta taaaggtttt gatatttaag ataaatacat
    14581 ggttattttt ataggtaatt tatataacga cattgaaata ttttaaataa acattagtaa
    14641 attattcatt catcaacatg atgataaaag ccggatgagt cagacatccg gcgagcagat
    14701 ttgttttaag gttccagttt tggatcaggt ccaaagcggt tttcgcctgg cgtacccgcc
    14761 tggcagttaa agacgatgat aataagccag ccgataaacg ggattaagaa tagcagtgcc
    14821 caccacgccg agcggtcggt gtcgtgcagg cggcggaact gaactgccca ccacggtaaa
    14881 aacaccagaa taccgtagat tgtcgtcagg atgccttcgc caccagcgcg ttgccagcct
    14941 aacattttat ccagcagccc cagcacgaac gtaaagatga tgttgaccag aataaacatc
    15001 cagtactctt tgcgccgcgc acgaccccgg aaaccgacat aattttttag tactttcaga
    15061 taccagtcca tttttgcctc aattttgctc aatcacttgt ttttaaagca gcaaattaag
    15121 aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg aattaataaa gtgctgtgaa
    15181 ggcgcgctaa gactgatgac aagcgcaaaa ttgccagatg cgctacgctt atctggcccg
    15241 cgcggtcatt gcaacatatt gaatataaaa gttttgtagg tttgataagg cgttcatgcc
    15301 gcatccgaca tgaacaacat gcacttagct aattatcaga ggcacctcgt aaggtgcttt
    15361 ttttaaccta agcgaacatc tcgtccatgc ggttcatcga gatcctgcca cggaccaatt
    15421 gaaataatcc gcgtagggtt gatggtctta tgactgcgga agtaatgatt acggatatga
    15481 tcgaaattga ctgtttcggc aattcccggc atctggtaga tatcacgcag gaagccatac
    15541 agattaaggt aatcgctgat gcggtgctta tcacacttga agtgggtcac atacactgga
    15601 tcaaaacgga ccagcgtggt ccacaggcga atatcggctt cggttagctg gttgccggtc
    15661 aggtaacgat gctgacctaa aatctgttcc agtcgcgcca gcgattcaaa cactttctcc
    15721 accgcttcgt cgtaggcttg ctggctggtg gcaaaaccag ctttatacac gccgttgtta
    15781 acggtgtcat aaatccagcc gttaagttcg tcaattttcg tttgcagggc tggtgggtag
    15841 taatcacccg ctttcgcgcc cagcgcatca aacgcggtat taaacatgcg gatgatttct
    15901 gctgattcgt tgctgacgat ggtgtggttc tttttgtccc acagcacggg aacagtaact
    15961 cgtccgctgt agtgtggatc ggcgtggaga taaagctgat acaaaaattc atgttgatag
    16021 agcgtgtctc cggttgctcc cggaaaactg tcatcaaagg tccagccgtt ttccagcatc
    16081 agcgggttca ctacggaaac ggaaataaac ggttccagtc ctttgagttt gcgcatgatc
    16141 agcgtgcggt gcgcccacgg gcaggcgagt gaaacataga gatgataacg atctttctct
    16201 gcgataaaac cgcctgtgcc agtggggcca ggagcgccat cggcagtgag ccagttacga
    16261 aatgcggaag ctgaacgttg aaatttaccg ccggtagatt tggtatcgta ccaggtgtca
    16321 tgccagacgc cgtcaatcag ttgacccatt gttctctcct ccaatagcaa aaacgaggag
    16381 cttcctcctc gttttttatc gttaagtata gcgcgtgctt accacttttt attcagcagg
    16441 cggtcgatgc tatacgcgcc cggaccggta attgccagca gcaggaatcc gccagaaatt
    16501 gtcaggtttt tcatgaacat cagcgagttg acgccttcag caaagttgct gtgaaataaa
    16561 aatgccgtca gcagcgtaaa gcccgcagta aacagggctg tggtgcgagt caggaaaccg
    16621 aacaggattg ccagaccacc accaaactca agcagaatca ccagcggcag cataaaaccc
    16681 gggacgccca tcgcttccat atattgttgg gtacccgcgt aaccagtaat ttttccccag
    16741 cctgcggtaa taaacagaat cggcattaaa atgcgcgcta ccagtacaca aacatcttct
    16801 aattttttca tcatactctc cactgcgcca ctcaggctgc tgattgtttt tttgtgcaag
    16861 ggcgcggtat tagcgtcgtt gctgtcgatg gagagaatca taaacgtggt gaatgatgat
    16921 tgttagcaag gaaaactgtc aaaaatcttc aaaaaatttg atgggtaagg ccggaatggc
    16981 tccggccaga gggaagttaa ccgcgaagct gttgctgctt gagggtcgtt ttaaccagac
    17041 gccaggcgct ccatacgcca aaaccgcgtc tggcccagcg gaccagcata ttaggatggc
    17101 gaatcgtcca gatcgccatc acgctactgc caaccagcgc ccaggagcgc agacttagca
    17161 gcatattcca gcgacgatcg taagcgcctg ttgcctccag ccattcacga cgactggcag
    17221 aaagatccag ccgttgttgc tggatctggc taagcagttg cgccttacgt cgttcacgtt
    17281 cgactttact gctcacggga ctcctcctcg agcagctgcc gatcgtttgc cagctcatgg
    17341 cgtgtatggc gcagcaaggt ggatttacgc gatttacgta acgtccagat accgccaatc
    17401 agtgccagta gcagcaacac cacggtggtg gcaatcatcg cattcaggcg atattgcgga
    17461 tcaaccgccc aaataattag caccatcagg ctcataagac caaatgcagc gaaaagcatc
    17521 gtcaggccca gcatcagtaa aagttgaaag agattagctt tttcctcttc cagctcaacc
    17581 accgccagcc gcagacgtgt ttccaccatt tcaaccatga tggaaacaat tcgctgcccg
    17641 atgcccagaa cgcttttacc gggcccttgc gcgtgatgag tgtccgccat aattaacgac
    17701 gcgacagcag aacgccgagc actacaccga ttgcagcgcc aatgcccacg cccgtccatg
    17761 gattttcacg cacatactca tcggcacgcg ccgccgcgac acgggtttgt ttggcgatgg
    17821 catcaccggt ttcacccagg cgataacggc tctgtttcag tgcctgctcc gctttgctac
    17881 gaatcttact caactcttct ttcgacttct cgccagatga gctaagcacc tcttccagcg
    17941 tatcggaaag ggatttcaac tcagcacgca gatgttccgt agtgtgttct ttcgacatag
    18001 ttttctccag gtgagtaagt agtgactatt gtgagttagt agtcgcgcgc ttccagcttt
    18061 ttcagctctt cctgcgcttc tgccagtttc cgttcgcgtt tggcaatctt atcggcatcg
    18121 cctttttgct tcgcctcggc taaatcttgc tggcgttccg ccacctcatc tttctgcttt
    18181 gcgattttct tctgatgatc ggcacgcagc tggctatctg aacagttggc ccggacttca
    18241 ctcagggctt tattcagacc gtcaatacga ttctggtttt ggtgtttttc ggcatagctg
    18301 atctccttaa ggatattttg ctccttttcc tgacacaggg tagtggcata actaccggca
    18361 ctaagagcaa aaagagaaac agctaaagcg atgcggtatt tcattcttga accttccatt
    18421 gtgattcgga cctattacaa aggaacgatt gtccagacaa cgaagcggta gtgttaacca
    18481 ccgcctcatt aatcatagac agttacggtg aaatcaccaa agtgagtgat ctgtttcgga
    18541 caacaggaag ttatccgaag cgatgagagt tatcccgtag ccgggtcagc cactgcatag
    18601 cctgactgtt attgtcctgc tgcgcaatga tgtagccttg gggcaatgtt ctgtcgagga
    18661 ccgcttttgc agcggcgctc tggtcgctgg agtcaaaggt aattaacaga gtgtcgtttt
    18721 tgggggtgat acttttgaaa ggaatgccat gagcgtcaag gtgatgccag attgaaaaac
    18781 cgtctggcat cgttgtgcct tgatgaacgg cacgaatcgc cagcgtagac tcttgctggc
    18841 gaaccgcgga ccaggccagc aacagcgtgc cgaccagaag taaaacagcg ccggaccagg
    18901 ctagctggcg aagcgacatg cgaggtattt gcatccctta cccccgattt ccatatttct
    18961 ttttccataa tacgaccaga gaacctgcca ggccaaacac cagcagcacc accgggagca
    19021 gcatcaggca tgacatcagc tggtcctcgt actttaaaaa taccggcgtt ttgccgagca
    19081 tgtaacccag agttgtcagg atcaataccc acagcagacc gctcatccag ttgaaaaact
    19141 gaaagcgcgc gttattcagc cctgataacc cggcaatcgt cggcagcagt gttctgacaa
    19201 acgcaataaa gcgaccaatt aacagcgccg ataaaccgtg tttatgaaaa agatggtgtg
    19261 cgcgttgatg ataatgcgcg ggtaaatgag atagccagtt ttgtacggtg cgggtattgc
    19321 ccagccatcg cccctgaata tagctgaccc agcagccgag gctggcggca acggtcagca
    19381 gcagaatcgt ttgcggatag cccatcgcgc ctttcgcaat caacacgccg accaatacca
    19441 gtaaactgtc gcccggtaaa aaggccgccg gaagcaagcc gttttcaagg aacaaaatta
    19501 caaacaagac aaaatacaac atgccaatca tcgatggatt ggccagggtt tcaaaatcct
    19561 gcgcccacag ggcttgcagc aattgggtca aaagttccat tcattattcc tggtgaatcg
    19621 gttaacgcca cgctggtttt aaaagatgca ctaccgcaat gttattgctg ttttggtatg
    19681 ttctgcgggt ttgatacatc aacacagcct gaactattcc ctgttaaatc gtgagataag
    19741 aaagcattca aaaacataaa ttcaaattga atctaattgt aacaaaggtc aacgttgttc
    19801 gttatcaaaa ttacatgtcc gtggagtctg attttgctga atgcagggga gggtggcgag
    19861 aggatttaca caggctgaca ctttatactt ttcgcttacc gacgagacaa acaggcgcgt
    19921 cgcagcgcct gtcggacagg tatttcattt actgccactg gtggccgtat cgagatgcac
    19981 taccgggttt tcggcgaaca gatagcggtc ggcattgaac tcgaagtcgt cgctggtttc
    20041 gttgaataac atgatcttgg tgttttccag gtgctgccac attgccagct tagcagcatg
    20101 aggatctttg cgaatcagcg ccttgaggat ttgatcgtgg tcatcacacc agttatcgac
    20161 ggtacgggaa tcaatgtgtt cgtgcagttt tttccagtac gggttatggc tacgctgggt
    20221 ccacattttt tcaacgatag ccgccagggc ggagttctgc gtcgccaggg ctacctgaat
    20281 atggaactgc aaatcccact cggaatcacg gaagcattgt tcgccgcgcg cctgctcctg
    20341 gatggccatc aatttcatga tgtcctgttt cgttacctga gttgctgcga actcggcaat
    20401 attactttcg atgagttggc gagcctggag taactcaaac ggaccgtaat tggcgaattc
    20461 catattattg tccgcagcct gctgatggcg cggctggttg gaaaccacgt gaatacctga
    20521 acctttacgc acttcgacat agccttcaac ttccagcatg atgatggctt cacgaactac
    20581 cgtacggctg acgttctttt catcggcaat aaagcgctca gcaggcagtt tatcacccac
    20641 cagatagacg ccctgttcga tgcgctcttt caggtcagcg gcaagttgtt gatacaaacg
    20701 gcgtggttca gtgatttcca tatgcgctcc aggcatgata cggcagacta tatttgttat
    20761 accacttttg cgagtttagc tctactcgca gtgatgaaaa agccgcctga gaaggcggct
    20821 ttgaagcgaa actaaaactt acacgaagcg ttaatgttgc ggtgcgggat cgttatgtgt
    20881 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
    20941 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
    21001 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
    21061 gttcgccgtt gccacttcgt tacgaccaaa cacgtcggaa gagagcgtaa tcagcgcgcc
    21121 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
    21181 ggtgaacagg ccgatcatac ccgggccaat catcagcact gcacccagcg ttacgaccat
    21241 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
    21301 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
    21361 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
    21421 ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag
    21481 gatctgacca acggacattt tcttcgccgt actcacctgg tgctgggctt cctgaccatt
    21541 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
    21601 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
    21661 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
    21721 agaacctacg ttaaaatagc ctactgcgat ggaacgctct ttcgccggga accattcgga
    21781 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
    21841 aacagccagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
    21901 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
    21961 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
    22021 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
    22081 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
    22141 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
    22201 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa
    22261 cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa
    22321 caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat
    22381 aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga
    22441 cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt taccactttg
    22501 agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg
    22561 aaattggtgt gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga
    22621 ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc gaatttgccc
    22681 gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc
    22741 cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggctgaaag
    22801 gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg
    22861 gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg
    22921 gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt atcactggca
    22981 aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc
    23041 aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca
    23101 ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca
    23161 ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa caggcgacct
    23221 ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg
    23281 acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt
    23341 ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa ttggacagca
    23401 ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa
    23461 ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc
    23521 acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag
    23581 gctttgactc catcaacgac cgtccgatgg cagaagagtt gtctaagctg ctgagcaagc
    23641 agaacgaaga aaacctgctg ccgaaaacca ttctgtactg cctgaacccg cgcgataacg
    23701 aagtgctggg caccatgagc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt
    23761 tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac
    23821 tggcgcagct cggtttgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc
    23881 tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc ggtcgctggg
    23941 tggaagcggg cgaagcaccg gcggacatca acctgctggg cgagatggtg aaaaatattt
    24001 gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat
    24061 gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcgg atttggctga
    24121 aggcacagaa gtcagtgtcg ataaccaaac tgttaggctg cgccaggatg ttgctcgtgg
    24181 acataaattt gcgttaacga atatcgcaaa aggggccaac gtcatcaaat atggcctgcc
    24241 gattggttat gcattggcgg atattgcggc gggggaacac gttcacgccc acaatacgcg
    24301 cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc
    24361 gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg
    24421 taacgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcagatcca
    24481 gaaccgtttt ctgaaagaga ccaacaacgc tgaaggtacc gacggcgtgt tcctcttcag
    24541 ccacacctac ggctgctcac agctgggcga cgatcacatt aatacccgca ccatgctgca
    24601 aaacatggtg cgccacccga acgcaggcgc agtgctggtg attggtctgg gctgtgaaaa
    24661 caaccaggtt gccgcattcc gtgaaacgct gggcgatatc gatcctgaac gcgttcattt
    24721 catgatctgc caacagcagg atgatgagat cgaagccgga atcgagcatt tgcatcagct
    24781 gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg
    24841 tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg
    24901 tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat
    24961 gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact
    25021 ggtcaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga
    25081 aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata aatcccttgg
    25141 ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg gcgagcgtct
    25201 gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc
    25261 cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccatacgg
    25321 cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga agaaaaaaca
    25381 ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga
    25441 agaatttatc gataccatcg ttgagtttgc caacggtaaa caaacctgca acgagcgtaa
    25501 cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt
    25561 gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat acctgataag
    25621 gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg
    25681 gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata
    25741 gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca
    25801 tccttaatgc cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga
    25861 tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat
    25921 tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca
    25981 tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt
    26041 cgataggcgg cacgatgatt gagggtagtt ttctgcttat gaatggcctg aatatcattc
    26101 gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa acccctcccg
    26161 ccatagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca gacgatcgtc
    26221 ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca gcgcggtttc
    26281 gcaagagtcc tgcaatacgc cgataataaa gccgacggca accacctgca tggcgatatc
    26341 gttcgaaata ccgaacatat tacaggccag tgggatcagc agcaaagaac cccccgccac
    26401 gccggatgcg ccacaggcac acagagaagc cactacgctc aacagcagcg ctgtgggcag
    26461 atcgaccgga atacccagcg tattaaccgc agccagcgtc aacacggtaa tggtgattgc
    26521 tgcgcccgcc atattgatgg tggctcccag cggaatagaa acggaatagg tatcgcgatc
    26581 cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag agctgcgggt
    26641 gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga acgggttacg
    26701 acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta acatacagcc
    26761 aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag
    26821 ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca gtttcaccat
    26881 aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt taccgtgacg
    26941 cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac ctttcagcag
    27001 cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt ctacaatgcc
    27061 tgacggtggc gaaatatcac ccgcgctact ggataagtgc agggtagaag ggaaggcgaa
    27121 gctgaaaacg actgcggcca gagcagcaga gaaggtaccc agcagataga ggaacaaaat
    27181 agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca tcaccagcat
    27241 caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac ctaacagacc
    27301 aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa caaggccgac
    27361 caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg gtgaacgttg
    27421 cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac
    27481 gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc
    27541 gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta atggttacat
    27601 ttatttgaca tgaaaataaa gccaacgtgc ggcttccaga ctgaggacta cgtcaaaaaa
    27661 ggactcggtc agtcccctcg ccccgtttgg ggagagggtt agggtgaggg gaacaggcca
    27721 gcatcggtgc gaaaaattac cctcaccccg accctctccc tggaagggag agggagaaaa
    27781 cggtgctcat gttgatgaca gggtacacgg cttaggcaga ttaaaaatca tccaacccgc
    27841 tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac caggatgcca
    27901 aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat cagcatcttg
    27961 ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa acgctctgct
    28021 acgcccgcca gcaggaaata catcgcacgc aggccgagga tcgcaaacag gtttgaggtc
    28081 agcacaatga acgggtcagt ggtcacggcg aagatagccg gaatgctatc cacggcaaaa
    28141 atcacgtcgc tcaattccac cagaatcagt accagcatca gcggtgtggc gtacagcaat
    28201 ccattcttac gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg caaatgaccg
    28261 cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg ggcgagcgcc
    28321 atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca gtcgaactgc
    28381 gaaatcagcc agctgccagt gaagatcatg atggtacgca gaacaatcgc cccgagcacg
    28441 ccatacacca gcacgcggcg ttgtaatgcc gccggaacgg agaaatagct gaacaacatc
    28501 agccagacaa agacgttatc gaccgccagc gacttctcaa tcagataacc cgtgaggaag
    28561 gccagcgcct gtggatcggc aacggcgcga ccttcggttt gtaccagata ccaccagaaa
    28621 gcagcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc agcctgtttc
    28681 atggtcatgg catgtgcccc acgacgtccc tgcaacaaca ggtcgatagc cagcataatg
    28741 gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt catagaagtt
    28801 ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    28861 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    28921 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    28981 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    29041 ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc tatctgccgg
    29101 aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    29161 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    29221 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    29281 cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    29341 ctgaatctcg cttgccagga cagaatcgct gacctttgag tgctgcaagg tgacgctgaa
    29401 atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccacttgcca gtaaactggc
    29461 ggttgcctgc acgcttttcg gctcaccaaa taacgctacc gccgacgcca ggcagtaaaa
    29521 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    29581 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaaa ctttccgcag
    29641 tttgccgact ttcggcagcg cctggcgtaa cagatgaaag ttaggcaggc tggccgtttt
    29701 aaatgcctca aacagcacca cctgattttc acgcgcgcag gcaatggctg catccacttc
    29761 cgccagattc gacgccagtg gtttctcaca aatcacatga attttgtggc tcaggaaaag
    29821 ttgcgtctgg gaaaaatgca gggcgttcgg gctggcaata tacaccgcgt cgatagcgtc
    29881 actttccgcc attgcttcca gcgaagtaaa cagatgctcg acagaaaact cattggcgaa
    29941 gtgctgggcc tgttcaaggc tgcgggaata aacggcggtt aacttatatt taccgctttc
    30001 atgtgcggcc tccacgaact ggtgagttat ccagttcgta ccaatcacgg cgaaacgtat
    30061 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    30121 ttcgacgtgc gcggatacgc attatttctg cgccggacaa ccatgctcac taaacggacc
    30181 aataatgacc gacggcccga gatattttgg ctgacgattg aagattttcg cgtcaatcac
    30241 cgcgctgact ctggcgagtt tctctcgcag acggacaatc tttccgcgtc ctttttccag
    30301 gtctggtgcg ttaaacccga tagcatcgat gccgtattgt tttgccagcc agatggcgcg
    30361 ttggttatga aattcctgcg agataatggt gatatggttc tccccaaaca ctttattggc
    30421 acgcaccact gaatcaagcg ttgagaatcc ggcatagtca cagaagatca cttttgccgg
    30481 tacgcccttt gcgatcaaag cctgctgcat gcctgacgct tcatcgtaat ttttacgtcc
    30541 gttatcgcca ctgaccagca gccatttcac ttttccggcg tggtacaact ctgctgcggt
    30601 atcaatacgt cgggtaaagt agcgattacc cggcctggcc cccagcaata agcccacgtt
    30661 acgcgccgga acgacgttga cgtcgctcca ggtcagctgt ttgctggcgt tgaccattac
    30721 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    30781 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    30841 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct
    30901 gacatcattt tcagattttg tcctgcccaa gcgacaactg ggttagccac agtcgggtgt
    30961 cgaactcaag ctggtggtac tggggttcca tatgacagca caactggtaa aacgctttgt
    31021 tatgctcttt ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt
    31081 ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc
    31141 cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat
    31201 tgtcatacat cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct
    31261 gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg
    31321 ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg cacctgggaa agtagctgct
    31381 cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg
    31441 gtatactcac gcaccctttt caggggcaat gtcgaaattt tatcattcag gagggccgat
    31501 gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga
    31561 cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac agttggacga
    31621 cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc
    31681 gctggcggaa cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg
    31741 cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc
    31801 cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct
    31861 ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgcca caaacacgta ttattgccgg
    31921 tgccaaagcc cgtgacatcc acacttccac gctggaacta ttcgaaaaag tgctcggccc
    31981 gaccaccacc acgctggcat ggaagaaagc gcgcctgatt aactgcactt tcaatgaacc
    32041 accgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat
    32101 ccacaaccat gcgaatgtct tctcccgcac cgggctggat atcggcgcgc gcttctttat
    32161 gcaacatctg ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt
    32221 tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc
    32281 gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga
    32341 tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc
    32401 tgtgctctgc aacccgccgt ttcatcagca acatgcgctg accgataacg tcgcctggga
    32461 gatgttccac cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa
    32521 ccgtcacctg gattacttcc ataaactgaa gaaaattttc ggcaactgca ccaccatcgc
    32581 cacgaataat aaatttgtgg tgctgaaagc agtgaagctg gggcgtcgtc ggtaagattg
    32641 acagctacct gccagataag gcgtaaacgc cttatctggc atacgtttag cacattaccc
    32701 ccaggtcgga taagatgcgg caagcgtcgc atccggcaat gtctgcccag ttctggaatc
    32761 gcgctccata aaacaaaaaa tccctgtcac catcctctac attctcttgt ttagcgttgt
    32821 ttaacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg tcaacggatg
    32881 ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata actcaaaaag
    32941 cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg gctctaggga
    33001 acacgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta ttcccatctc
    33061 tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc aatgaattac
    33121 gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa gttatgacgc
    33181 ataaagaata cgattttttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat
    33241 tgccgacatc ttgcatgcag gagaaaagct aactgctgtg gcaccttttc tggcgggtat
    33301 tcagaacgag gaacaataca tccaggcgct ggaactggta gatcatctgc tgctcaacga
    33361 tcctgaaaac cccttactgg atctggtgtg tgccaaaata accgcgtggg aagaatcagc
    33421 gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat
    33481 tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa
    33541 atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa
    33601 attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg a
//
LOCUS       DAZGXS010000037        33470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGXS010000037 DAZGXS010000000
VERSION     DAZGXS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..338
                     /locus_tag="ACHKG5_001930"
     CDS             <1..338
                     /locus_tag="ACHKG5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0868041.1"
                     /translation="ISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGR
                     ECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSAGPEITTPWKSYQHEKNRNGLMPL
                     IRRRTMLQL"
     gene            528..641
                     /gene="yhiY"
                     /locus_tag="ACHKG5_001931"
     CDS             528..641
                     /gene="yhiY"
                     /locus_tag="ACHKG5_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0868042.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            704..1771
                     /gene="yhiI"
                     /locus_tag="ACHKG5_001932"
     CDS             704..1771
                     /gene="yhiI"
                     /locus_tag="ACHKG5_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0868043.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1768..4503
                     /gene="rbbA"
                     /locus_tag="ACHKG5_001933"
     CDS             1768..4503
                     /gene="rbbA"
                     /locus_tag="ACHKG5_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0868044.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4503..5627
                     /gene="yhhJ"
                     /locus_tag="ACHKG5_001934"
     CDS             4503..5627
                     /gene="yhhJ"
                     /locus_tag="ACHKG5_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868045.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5700..5975
                     /locus_tag="ACHKG5_001935"
     CDS             5700..5975
                     /locus_tag="ACHKG5_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0868046.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5972..6331
                     /locus_tag="ACHKG5_001936"
     CDS             5972..6331
                     /locus_tag="ACHKG5_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0868047.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6381..7241)
                     /locus_tag="ACHKG5_001937"
     CDS             complement(6381..7241)
                     /locus_tag="ACHKG5_001937"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFP0868048.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7273..7542)
                     /locus_tag="ACHKG5_001938"
     CDS             complement(7273..7542)
                     /locus_tag="ACHKG5_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0868049.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(7532..9040)
                     /locus_tag="ACHKG5_001939"
     CDS             complement(7532..9040)
                     /locus_tag="ACHKG5_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0868050.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9033..10391)
                     /locus_tag="ACHKG5_001940"
     CDS             complement(9033..10391)
                     /locus_tag="ACHKG5_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0868051.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10468..10749)
                     /locus_tag="ACHKG5_001941"
     CDS             complement(10468..10749)
                     /locus_tag="ACHKG5_001941"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0868052.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSNLKK"
     gene            complement(10746..11219)
                     /locus_tag="ACHKG5_001942"
     CDS             complement(10746..11219)
                     /locus_tag="ACHKG5_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0868053.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11244..11990)
                     /locus_tag="ACHKG5_001943"
     CDS             complement(11244..11990)
                     /locus_tag="ACHKG5_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0868054.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12189..12590)
                     /gene="nikR"
                     /locus_tag="ACHKG5_001944"
     CDS             complement(12189..12590)
                     /gene="nikR"
                     /locus_tag="ACHKG5_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0868055.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(12596..13402)
                     /gene="nikE"
                     /locus_tag="ACHKG5_001945"
     CDS             complement(12596..13402)
                     /gene="nikE"
                     /locus_tag="ACHKG5_001945"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP0868056.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(13399..14163)
                     /gene="nikD"
                     /locus_tag="ACHKG5_001946"
     CDS             complement(13399..14163)
                     /gene="nikD"
                     /locus_tag="ACHKG5_001946"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0868057.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14163..14996)
                     /gene="nikC"
                     /locus_tag="ACHKG5_001947"
     CDS             complement(14163..14996)
                     /gene="nikC"
                     /locus_tag="ACHKG5_001947"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0868058.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14993..15937)
                     /gene="nikB"
                     /locus_tag="ACHKG5_001948"
     CDS             complement(14993..15937)
                     /gene="nikB"
                     /locus_tag="ACHKG5_001948"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0868059.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(15937..17511)
                     /gene="nikA"
                     /locus_tag="ACHKG5_001949"
     CDS             complement(15937..17511)
                     /gene="nikA"
                     /locus_tag="ACHKG5_001949"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0868060.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(17622..18209)
                     /gene="acpT"
                     /locus_tag="ACHKG5_001950"
     CDS             complement(17622..18209)
                     /gene="acpT"
                     /locus_tag="ACHKG5_001950"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0868061.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18211..19440)
                     /locus_tag="ACHKG5_001951"
     CDS             complement(18211..19440)
                     /locus_tag="ACHKG5_001951"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP0868062.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19437..20168)
                     /locus_tag="ACHKG5_001952"
     CDS             complement(19437..20168)
                     /locus_tag="ACHKG5_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP0868063.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20168..20632)
                     /locus_tag="ACHKG5_001953"
     CDS             complement(20168..20632)
                     /locus_tag="ACHKG5_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP0868064.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(20629..21798)
                     /locus_tag="ACHKG5_001954"
     CDS             complement(20629..21798)
                     /locus_tag="ACHKG5_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP0868065.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(21800..22384)
                     /locus_tag="ACHKG5_001955"
     CDS             complement(21800..22384)
                     /locus_tag="ACHKG5_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP0868066.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22381..24699)
                     /locus_tag="ACHKG5_001956"
     CDS             complement(22381..24699)
                     /locus_tag="ACHKG5_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP0868067.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(24668..25273)
                     /locus_tag="ACHKG5_001957"
     CDS             complement(24668..25273)
                     /locus_tag="ACHKG5_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP0868068.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25270..25692)
                     /locus_tag="ACHKG5_001958"
     CDS             complement(25270..25692)
                     /locus_tag="ACHKG5_001958"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP0868069.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(25696..27372)
                     /locus_tag="ACHKG5_001959"
     CDS             complement(25696..27372)
                     /locus_tag="ACHKG5_001959"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0868070.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(27363..27716)
                     /locus_tag="ACHKG5_001960"
     CDS             complement(27363..27716)
                     /locus_tag="ACHKG5_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP0868071.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(27703..29064)
                     /locus_tag="ACHKG5_001961"
     CDS             complement(27703..29064)
                     /locus_tag="ACHKG5_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFP0868072.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICG
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29061..29642)
                     /locus_tag="ACHKG5_001962"
     CDS             complement(29061..29642)
                     /locus_tag="ACHKG5_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868073.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(29647..29898)
                     /locus_tag="ACHKG5_001963"
     CDS             complement(29647..29898)
                     /locus_tag="ACHKG5_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0868074.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(29910..30167)
                     /locus_tag="ACHKG5_001964"
     CDS             complement(29910..30167)
                     /locus_tag="ACHKG5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP0868075.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30142..30963)
                     /locus_tag="ACHKG5_001965"
     CDS             complement(30142..30963)
                     /locus_tag="ACHKG5_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP0868076.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(30960..31682)
                     /locus_tag="ACHKG5_001966"
     CDS             complement(30960..31682)
                     /locus_tag="ACHKG5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP0868077.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(31723..32781)
                     /locus_tag="ACHKG5_001967"
     CDS             complement(31723..32781)
                     /locus_tag="ACHKG5_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP0868078.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(32850..33227)
                     /locus_tag="ACHKG5_001968"
     CDS             complement(32850..33227)
                     /locus_tag="ACHKG5_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0868079.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         7777 a         8931 c         9138 g         7624 t
ORIGIN      
        1 ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac
       61 ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt gcacataacg
      121 gtaatggtgg aagagaatgc ataggtgaaa cggggacagt tttaggtctt aacgcccgtg
      181 acagtaaaca tacgggaaga atgttcagca tgcataattg ccaaattaac cctagtgcgg
      241 ggcctgagat aaccactccc tggaagtctt accaacatga aaaaaacaga aatggactta
      301 tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta
      361 atgttactat ctggttgaga ctgttgataa aacgtaataa ggaatgctct gttcctgcaa
      421 ctgaacaggc gtgaaacgct atccaacagg atggataccg ttttgtcagc cagttttata
      481 gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
      541 tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta
      601 tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt
      661 gtaacacgcg tcgtttgctt acagggatta tggcgggtcg attatggata agagtaagcg
      721 ccatctggcg tggtggggtg tcgggttact ggcggtggct gctatcgtag cgtggtggct
      781 gttgcgcccg gcaggtgtgc cggaaggctt tgccgtcagc aatgggcgca ttgaagcgac
      841 ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg
      901 ccagtttgtt cgcgaaggcg aagtgctggc gaagatggat actcgcgtgt tgcaggagca
      961 gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc
     1021 tttgctggag caacgacaaa gcgaaactcg cgccgcacag tcgctggtta atcaacgcca
     1081 ggccgaactg gactccgttg ccaaacgtca tacgcgttcc cgctcgctcg cccaacgagg
     1141 ggctatttct gcgcaacagc tggatgacga tcgcgccgcc gctgagagcg cccgagctgc
     1201 gctggaatca gcaaaagccc aggtttcggc ttctaaagcc gctatagaag cggcacgcac
     1261 caatatcatt caggcgcaaa cccgcgtcga agccgcacaa gccactgaac ggcgcattgc
     1321 cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt
     1381 tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag
     1441 cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga aacttggcgg
     1501 tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa ccatcagttt
     1561 tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa
     1621 actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     1681 tgtcaaaacg ggcttgccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     1741 tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct
     1801 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc
     1861 acactcgata ttccggcccg ctgtatggtc gggctgattg gtccggatgg cgtcgggaag
     1921 tcgagtttat tgtcattgat ttccggtgct cgtgtcattg agcagggcaa tgtgatcgtg
     1981 ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg
     2041 atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga aaacgtcgat
     2101 tttttcgctc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat caatgagctg
     2161 ctgaccagca ccgggttagc accgtttcgc gatcgtccgg cagggaaact ctccggcggg
     2221 atgaagcaaa aactggggct gtgctgtgcg ttaatccacg acccggaact gttgattctt
     2281 gatgagccta cgacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac
     2341 agtattcgcc agcggcagag caatatgagc gttctggtcg ccacggccta tatggaagag
     2401 gcggaacgct tcgactggtt ggtggcgatg aatgccggag aagtgttggc aactggcagc
     2461 gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt tataaatctg
     2521 ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccata tcaacctgaa
     2581 aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     2641 gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct tggttcgaac
     2701 ggctgcggta aatccaccac catgaaaatg cttaccggat tgctgcccgc cagcgaaggt
     2761 gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatactcg ccgtcgggtg
     2821 ggctatatgt cgcaggcgtt ttcgctctat aacgagctca ccgtgcggca aaaccttgag
     2881 ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcaagagt ggctgaaatg
     2941 agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt gccgctcggc
     3001 attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggaaat gttaatcctc
     3061 gatgaaccta cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtc
     3121 gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat gaacgaagcg
     3181 gaacgctgcg accgtatctc tctgatgcac gccggaaaag tgctcgccag cggtacaccg
     3241 caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg
     3301 caggaagcgg caggacagag caacgaagcc gaagcgcctc ccgtgataca cgacaccaca
     3361 cacgcgccgc gccagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagcg
     3421 ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac agtgatcctg
     3481 atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctt
     3541 gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     3601 tttatcgaac agccgctgct taccagttat gacgagcttg atcgtcggat gcgtgcgggc
     3661 gatatcacgg tggcaattga gatcccgccc aatttcgggc gcgatatcgc gcgtggtacg
     3721 cctgtggaac tcggtgtctg gatcgacggt gcgatgccga gccgcgccga aactgtaaaa
     3781 ggttacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg acaatccaca
     3841 ccctccagcc aaagcgggct gatgaatatt gagacgcgtt atcgctataa cccggacgta
     3901 aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     3961 atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac
     4021 gtgaccccca ccacccgtag cgaatttttg cttggcaaac agctgccgta catcgcgctg
     4081 gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat
     4141 aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat cgccaccgga
     4201 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggtacggcg
     4261 attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     4321 gaagggccgg gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     4381 cgcgggacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg
     4441 ctgatagcca taccgctggt gatgggctta agcatcctgc tgctgaaaaa acaggaggga
     4501 tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctactcg
     4561 gtgataaagc gatgctgaca ttgattgtct tctcgtttac ggtgtcggtg tattcgtcag
     4621 cgaccgtcac gccaggatcg ttgaacctgg caccgatcgc cattgccgat atggatcaat
     4681 cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga
     4741 tgatcaccgc cgatgagatg gatgccggac tggacgccgg gcgttatacc tttgcgataa
     4801 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccagat attcaggtga
     4861 acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta
     4921 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     4981 cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga
     5041 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc
     5101 gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga
     5161 tcatgatggc gaagatctgg tcgatggggt tggtggtgct ggtggtatcg ggattatcac
     5221 tggtgctgat ggtaaaaggc gtgttgggcg taccgattga aggctcgatc ccgctgttta
     5281 tgctgggcgt ggcgcttagt ctgtttgcca ccacgtcaat cggcattttt atggggacaa
     5341 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa
     5401 tgctttccgg tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     5461 tgatcatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac cgtggtgccg
     5521 gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca
     5581 ccattgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt
     5641 gacttcatac tgtgatgtca tactattgat gtcataaagt gaagtcataa ggacatgcca
     5701 tgagaaccaa ggtacaggct ttacggaaga aacaaaaaaa tacattggat caaatattta
     5761 aaactcctgt tcctcaaggg atcaagtggt ctgatataga gtcactggtt aaagcattag
     5821 gcggagaaat taaggaagga agaggttcgc gttgtaagtt catactaaat atgagcgttg
     5881 cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc
     5941 gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat
     6001 ggagattgcc gggcaacctg ctgttatcac ttacgtgcca gaactgaatg catttcgtgg
     6061 caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca
     6121 aaaagaaggg gaactttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga
     6181 gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag
     6241 tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga
     6301 aacactgaat gagcgtttga atcatttata acttatagcg gcccaacaat ttctgttgcg
     6361 ccgctatatt tttgtggctc ttaagatgcc tttctaacca accgatgccc ctcattcatc
     6421 atcaggattt tgctatacac atcttcctcg acggcgtttt ttgctgatgc catgactcgt
     6481 gccagattcg cctcgttgtg attattcaca tacgctttgc cgacggcggt gataaacgct
     6541 ttgcgcagat cgctggcgat atttacttta gcgacgcgat aattaacaaa gctgcgtaat
     6601 atctccgggg cgataccgga gccgccgtga ataaccaacg gcaccggaca gacttcggca
     6661 atgcgcttca gcagggggat atcgatacgc ggtatatcgt ccaggccatg aacgttgcca
     6721 atagaaactg ccagcatgtc gcaaccggta cgctcgacaa aggtcttcac cttttccggt
     6781 tccgttttgc aatcggcttc gctgacgtga tcatcttctt tgccgagaat cgcccccagc
     6841 tccgcttcaa ccggtacgcc ataggattta cagaaatcga cagcttcctg ggtgaaagcg
     6901 atattctctt caaaagagaa tgcggcaccg tcaatcatga ccgaggtaaa gcccgcgcgc
     6961 accgcctgtt taacatcctc ctgggttttc ccgtgatcca gatgaagact gaccggaaca
     7021 tccattttat cggcgtgacg tttcacgatt tcataaatgt aatcgtatcc cgagagttgt
     7081 gcattggttg gcgcaatctg aatgaagttg ggtaatcccg aacgctcaat agcgtttaaa
     7141 attgaaattg tcgtctccag attggtggta ttaaatgcgc ccgctaatac ccgtttttcc
     7201 tgaatgcggt cgagcagtat ccgtccatta actaatggca tattgaaaac ctcatctttt
     7261 aatatcagtt aattattcag cttcaccgaa gcggtcgcga aataagtcgg tgagagtctg
     7321 tatggcctcg ttttcatcat caccgtcgca gcgtatttcc accgtttttc cgccttttat
     7381 tccggcgcgc ataatattca tcatgctggt ggcattaata tcattaccct catagtgcag
     7441 cgtcacttta cagctatatt ttttggcggc tttcgccaga caggccgccg gacgtgcatg
     7501 caggccagta ctgtttctga cctccacggt tttattcaac atagtgttcc tctcgatgat
     7561 gtaatggcat cacgccttct tgttgtaatt tcaactgata ccagcttttc catttgtgct
     7621 gataacgctg taattgatgg tatctcgccc gattcggtaa caaacgttgg gctatagcga
     7681 ctggcggcgg tggaatatcc ggtgcggcgg ttaataatgc tccgacagaa accgcctcgt
     7741 taaatgcaat tgcttcaacc ggacgctgta aaatatccgc ctttaattgc agccaaagag
     7801 gattatgggt tgccggaccg atcactttta acgcgctggt gccaggcacg ttaaaacagg
     7861 cgaagcagtt ggcaaattcc atggttaatc ccagtgatac gctggcaatt aacatgtcga
     7921 tacttaacgt atcgccaagc ccataaaata atccacgggt atggcgattt ttatagggcg
     7981 aacccgaacc gcgaagatgt ggaataaaga cgggaatgtc atcgagcaac caattccccg
     8041 ccagataatc agcatgtccg cgagtaagtg atgcggcgat ctcctcatcg gttagccgga
     8101 acgtgttgcg cagccactcc agcgcgaaac cgcccacggg cagcgacgca aacagggtga
     8161 actggttggc aagtgagtaa cagccgtttg ccagcttgtc acgtttggcc tgttcatcca
     8221 gcgtcggttg tgtatccagt tgcagaatgc cttccgtggt ccccgtcgag ttaaggatat
     8281 cgcctggcat catctgcaac gctcgcgcac ccaccatatg gtcatgtccg gccagcgtca
     8341 cgctgaccgg ttgcgagaac ccaagcgtct tgcaaagtga ctcgctgacc catccggcgt
     8401 gctcgcctgg ctgcaccaat ggcgcaaatg ccgtacaggg aacgtgtaac aacgccgccg
     8461 ctttcgctga ccattcgcct ttgcgcacgt ccagacacag cgtgcggctg gctaaggtga
     8521 tatccgtgtg ctgttcgcca gtcagccgcc agagcagcac ttccggcgca tgtagccagc
     8581 gcaggccgcg gcgattacgc agcgggtaat gttccagcaa ccagcgcatt ttgaaggcag
     8641 agtaattgct gtgtagtggt agaccgcaga tgtcataaag tgccgcactg tctgcctcgc
     8701 taagcgttgc cagatactct tcaccgcgac ggtcatacca tgccagcatt ggcgtcagga
     8761 tctcgccatg ctcgtcaagg aacacgcctg attcgccaaa actggcaatg ctgatccgtc
     8821 tgactggcag cggcgaagcg gcgttcagtt gcgctatcgc ctggcggacc tcctgccaga
     8881 gggcgtcgat atcgaaatcg acatcgccct gtggggagat ctgctttgcc gtcacgaatt
     8941 tatgcgcgcc caacgtcgtt gcgtccaggc aggaaaagca ggtgactttg caattggtgg
     9001 tgccaatatc gataacgata gctgcgctgt tatcaggcat aaaccgtgct ccgcgtcatt
     9061 cggaaaatgc catacagtac cagggcgata accggcacca gaccaatggc gatgagattg
     9121 ccgttgaaga tttccagcag ccagaagcgg aacgggttac cgccatcaag aaaactggaa
     9181 acctggccgc tggagccttc ggcaaatttg aagttaacgt ctttcgccat tccggtaata
     9241 aacggcgcga ttttggtagc aatccatagg tcggcaataa tcaccgggat cgccgccaga
     9301 acggtgcgga aaatattgcc acggctggct aaagcaatca tcgacgccat taccgccagg
     9361 ttggcaaggt cgccaagcgg caggacttca ttacccggaa ggacaaacgc gataaagacc
     9421 gagattggcg ttagcagcag ggcggtggag ataatcgccg gattacctac ggcaacggcg
     9481 atatccaggc cgatatagag atcgtcacgg tcagggtatt tggcattgag atacttttta
     9541 atagcttcag acagcggtaa taaaccttcc atcaggatgc gtaccatgcg tggcaggatg
     9601 aacatcacac cgccaatgct gatgccaagc agcaatactt ttttgaaatc gtatccggca
     9661 attacgccga gcagaatgcc cagaatagtg ccaaccatca tcggttcgcc gaagatgcca
     9721 aactttttct gtacggtttc cgggtcgata tgaatacgat tgaggccagg gatatggtcg
     9781 atgattttgt cgaccagcag accgaccggg aagaacacca ccgaagagag cgtcggcaat
     9841 gagatccctt ccagtccaaa gtatttttgt accagtggcg cagaccagtc ggcgagttta
     9901 agtactacca ccgcagcaat cgccgccgcc agcaaaccaa agaacaggct gccggtgctg
     9961 taataaacca acgtaccagc aagggcaaaa tgccagtagt tccacatatc tacgtcaacg
    10021 gtacgggttt tatttaacgc cagcatcgcc acgttaatca gaatggtcag gggaataaca
    10081 aacggggcaa ttggcgaacc ccatgtaata gcggaaagcg gcggccagcc taaatctacc
    10141 accggtaaat taattccggt gcgttcaacc atcgcctggg ccgctggccc gacattgctg
    10201 gtcagaatat cgaaaatggc ataaataccg acaaaaccaa tgccgactgt taatgacgaa
    10261 cgtaaggctt tagcgggttt tactttaaaa cagagagcaa gaataaataa caccaacggc
    10321 aacattaccg tcgggccaaa tcccaatata tattgcacaa tattatagag ggtatgcgcg
    10381 atatcgttca taataaacct ctttttcagg cgagagcgca cctcgcgtta gcgcctgatg
    10441 gagtgggttg aaattaatta ttattaatta ctttttaagg ttagaaacaa tttgcgccag
    10501 tactttttct tcgccaatac cggtaataat cggcaagccg ttcaccaccg ggacgtcgag
    10561 ttcataaggc actttagttg ttgaaactac cagtgctacg ccgtcagtat ttccaggtaa
    10621 agaggcaacg ttggtttggg aataattgac gttaagacct tgctctttgc aatattccat
    10681 gactttttct gttaccgccg tggatgtggc aatacccgtg gcgcagacaa ataaaatggt
    10741 ttgactcata tttcacctcg ttctttcatt ttgtttaaga ttactgtgtg tatttctgtt
    10801 gtttccatcg caagcagggc ttgcataaag ggcgtatcct gaatcatttc cataatccag
    10861 cccagggcat ttaattgttt attgctttcg cttaaggcga gcaaaaaaat caccctgaca
    10921 ggtacggggt caggatcgcc gcccatatcg gcaaactcta ccggttccgg caggatgccg
    10981 acggcaattg cattgtgcct gacatgttta tggtccgtgt gcggaatagc gacgcacggt
    11041 ggttcggtag gcagccccgt tgggtacgtc gcctcacgtt tcagtacgtg atcggcaaaa
    11101 tcagggacga ccagctcggc ggcaaccaac ggttgcgcta actggcggat gatctgttca
    11161 acgcttaacc ctgccggaag tcgttgaata taattttcct gaaaatgtat gccttgcatt
    11221 ttattcctct taaaaaagtg tacttaactc acttcccgcc gttgcagtac ggtgccaaga
    11281 taatatttat ttccttttaa ccagacgttg ccaaactcaa tgggtaatcc tcgggagaaa
    11341 aagactaact gctccagatg caaaacgggt gcgtcttcac tgatatctaa aaaatgacca
    11401 cgttcattac caattaatcg cgcggcatag cgactttccg agtaacgaat ttttctttgc
    11461 gacaaacttt ctattgttgg aaataaattg tgttgattaa aatcgatttc agcaataccg
    11521 gggcatagct caatattgat acggttctct atcagcatcg ctttttcatc accaattgag
    11581 cgtaagcgtt caaggtaaag aatatcctga ccgggcgtta ttcttaattt ctctgccaca
    11641 taacgattag ccgattcaat ccgtgacgta ataacttcag tggtaaaatg tattttctgg
    11701 ctttccagcg attccgcgaa tgacaataat ccttcaccta acggatatgc cacgttctct
    11761 tttttgacaa atgttccctt accctggacc tgtattaaca cgccttcttt taccagttgg
    11821 gcgaccgctt ttttaacggt tccccgactc acgtccagca tatccataat ctggtattcc
    11881 gaaggaatgc gatcgtcttc caccagatct ccggtataaa tactttctct tatccactcc
    11941 accacttgtt gatagagcgg tatttgtgac gacttactta atgatttcat ggcgcgcacc
    12001 tgaacattta cagttgtcta tggacaatca tggacaactt caagcgtata gtaggtgaac
    12061 gggagaaaaa gaggatcgaa gcgtctcaca ttttgaggga ccaaatcgtg atcaaaaaaa
    12121 tgtgacgtga gtcgatattt gtcagaagtt tactgcgccg taaggaaata acggcgcgtt
    12181 ggtaggtgtt aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg
    12241 gcgataacgt catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt
    12301 tccagacagt cgtcgtggtt gatgtgcaca tgtagcgtgg cgacggagag gtcgtggtga
    12361 tgatgctggg tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac
    12421 gacagcaccg cgaaaccttg tgtgccgtgc tgctgggtag cttcctgcgc cagggcgcta
    12481 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
    12541 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgttaaa
    12601 ccttttctgt ggcgcgacgg cgcacgggga atgcgggtaa tatcgcgttt tgtagcacac
    12661 gtccggcgtc agaggaaaag gttaatttct ctcccaccgc ctgggtttcg acgatttgtc
    12721 cgttgtccat caccatcact cgctggcaaa agcgttccac caggcgtaag tcatgagtga
    12781 tgaacaggca ggcagtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
    12841 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagcaat ttcggttcga
    12901 ccgccagcgc gcgtgccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc
    12961 gcttgtcgag cacgctgtcg tcgagatcga ccgcgtgcag catttcgctg gcacgcgcca
    13021 gttgttcgga ttttttcagc gatagcaggt ggcgcatcgg ttcccgcagg atctcgcgca
    13081 cggttttgcg cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc
    13141 ggaacgcttt acgctgggcg cgattgagct ttgccaacga ttcgccacgc cagctaatat
    13201 ttccctgtga gggcgattct aagcctacca gtagccgcga gagagtgctt tttccgcagc
    13261 cgctgcgtcc cagcagggcg acggtttcac cgcttttcag ggtcagggaa acgttattca
    13321 gcaccgcctg gtgctgatgt tttccgctaa acccaccgtg ggcatagtga tgggaaaggt
    13381 cggagacgtt aagtaaagtc atgatgccag ctccataccg tagagggcga gatgagcgga
    13441 aaccaggctg cgcgttaccg catgtttggg ggcgttaaac agcgtttcga catcgccctg
    13501 ttcgacaatc ttaccgtcag acattaccgc tacgtcatcc gccagacgcg ccaccacgcc
    13561 catatcatgg gtgaccagca gcatccccgg cgcctgtttt tgcataatgc tttccagcag
    13621 atcgagaatg cgtgcctgtg ctaccacgtc gaggtcggtg gttggttcat cggcgatgat
    13681 aaacggtgat tcgcaaagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
    13741 catctcgaac gggtacagct tcagcacgcg tgcggcgttt tccagcccca ccgcttctat
    13801 ggcagcggta agcgtagcgt catcggcggg tttccccagc gccaggcacg tctcccgcgc
    13861 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
    13921 aattttgatg ccgcgcaggg cgcagggaga aaccggtttg ccatcggcta aaatttcccc
    13981 cgccgtctgg cgaacgcctg cgggcagaat tcccagcgtc gcggcgcagg ttaatgattt
    14041 cccgctgccg ctaccgccga ctaacgccag cacgcgcccg cgtttcaggg ttaacgacac
    14101 gccgtgcacc agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg
    14161 cattagtgtg cgtgctccgt caccagatga ggatccagat gatcgcgcag tgcgtcaccc
    14221 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
    14281 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
    14341 gcggtcacgc cgaggccgag gaaggacatc ccggcaacgt gcagcatcat atggccgata
    14401 tccagcgttg ccagcaccag cagcgaaggg atcaccgcac ctgccagatg atcaacaaac
    14461 actcgcacat ggcccgcgcc agaaagccgt gatgccagca caaactcgcg ctggcgcagt
    14521 gaaatcacca ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
    14581 ataattacgt tggtcagccc agtgccgagc acgccaacca taaagaatga cagaatcgag
    14641 gtcgggaagg tcataaacat atcggcgacg cgcatggtag cctgatcaac gcgcccgcca
    14701 atcagcccgg cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc
    14761 attaccgaac cgagcgacac acgggtcgct gccatcagcc gcgagaaaat atcgcgccct
    14821 aagtgatcgg tgcccaacca gtgctgcgca tccggcgaaa gcaggcgcga cggcaaatca
    14881 atcgcctggg ggtcatacgg cagccaccac tggctggtga gcgcaatcag cgccagcagg
    14941 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
    15001 gcgctccttc atgacgacga atgcgtgggt ccagtgcggc gttgagcaaa tcgacaatca
    15061 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt
    15121 cacggttaaa aatcgccgac accgcatagc gcccgacgcc gggccaggta aagatgtttt
    15181 cgataatcat cgtcccgcca atcagttcgc caatgtgcat ccccacggcg gtgatcatcg
    15241 gcagcgaggc attgcgcaga atatgacgac gctcggtctg tttgtcattc aggccgcgta
    15301 gccgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagcagcc
    15361 gtgcgttaat cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc
    15421 agccgccgta gcccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta
    15481 aaaatgccag ccagaagttg ggcatcgaca cgccgagaaa cgcgatgaaa cgtacggcga
    15541 aatccggcag acggtcacga tggcgcgccg cccagatacc gagcggtacg gaagtgagca
    15601 gaattaatac cagcgccgca cccgccagtt ccagcgtggc gggcaggaag ttcagcatat
    15661 cgtccagcac cgggcgctgc gtggcaaatg agatgccgaa atcaagatgc agcgccttcc
    15721 acaaccaggt gccgtactgc acatacagcg gctgatccag tcccagcatg gtgcgggtag
    15781 aggccagcat ctccggcgtc ggcggcaggt tagacagacg caaataatcg agcgccgggt
    15841 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
    15901 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
    15961 ttcgaacgga atttcggtgg cgatcggcgc gtaggggatg ttacccagct ccggttttga
    16021 taccaccatc attgagatgt aactgatagg cagataaacc gcctcgtcat gcagacgggt
    16081 cagaatgtcg cgatacagtg cctgacgttg tgcttcgtca tgggtcgcca gcacttcgcc
    16141 gatctcttta tcaatcagcg gcttgtcggc taatccttgc tgtgcctgga agtcagcgtg
    16201 cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
    16261 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgcgca tagatactgc tctcttcttc
    16321 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
    16381 cgatttgctt aacgcatcgg tgccgatgaa cgaaagttca atgtgcagcg gctgaccatt
    16441 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt
    16501 cgctttctgc ggatcgtact ggcgcggttt caggccgagg ttggcgtagg gcacagaagg
    16561 ggcaaacagg gtgtcggcga cctgctgggt gccatacaac gcgttatcaa tcagcgattt
    16621 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcattggtgg gggctttggc
    16681 ggtattaagc gccagcatca cggtttcgat cggctgtgac agttgggtat ggtaagccgg
    16741 gttctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
    16801 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtctggga tgacgttaaa
    16861 agtgatcttc ttaattgccg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
    16921 gtactgattc agtttcgatt cctgcaaaac ccacggtccg gtgccaatcg gcgctttaat
    16981 cccgttcatg gtttcatggt ttttaaactg cgagggggcg ataaagcgga aagggcgcgg
    17041 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtgattt gcagctctgt
    17101 tttactgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
    17161 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tctgcatcaa acggttcgcc
    17221 gttagagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
    17281 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
    17341 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
    17401 cccgacattc accggccagg cggtggtgat ttcatctggt gcagcggcat ggacgataaa
    17461 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc
    17521 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
    17581 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
    17641 cattgcacac tgtcggcggt gagcgtaaag ggagtagggg tgcagatcgc caggcttaaa
    17701 ttttctagct gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg
    17761 atttgccagg cgctgccacc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
    17821 cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcagcatg ttccccgagg
    17881 ctgaacacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg
    17941 cagccgactt cgccttcatc actcaacagc agggcgatat cgtcaccgct atggcttaag
    18001 ttgaaccata gcggcgtttc cggcgcaaat gcaggtttgc cttgttcgcc atagatgatc
    18061 tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt
    18121 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
    18181 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatga tgatggatgt
    18241 attaatgccg ccaaaggcaa agttgttact ctgcaaaaac tcacaatcaa ccttgcgggc
    18301 ttcatgcatg atgtaatcca gcgcgccaca attcgggtct ggtttattca ggtttagcgt
    18361 aggcgcaaac caaccttcgc gcatcatttg cagactcatc caggcttcga gcgcgccaca
    18421 ggcaccaagc gtatgaccaa aataactttt cagcgaggag agcggcacat tatcaccgta
    18481 aatggttgct gtcgccagac tttctgccat atcaccgcga tcggtcgccg taccatgcgc
    18541 tgaaatatag ccgatatctt gtgcgcttaa cccggcgatt ttcagcgatt gctccataca
    18601 atactgcatg gtttcgcgct gcggctgggt aatatgtgcc gcgtcgcagt tagtcgcaaa
    18661 gccgacgatt tcaccgtaaa tggtcgcgcc gcgcgctttg gcgtgctcca gttcttcgag
    18721 aatgagcgta cctgcgcctt caccaatcac cagcccgtcg cggttttcat cgaacggtga
    18781 aggtgtggtt ttcggcgcat cattgtgctg gctggtggcg aaaagcgtat caaataccgc
    18841 tgcttctgaa ggacataact cttcggcacc accagcaacc atcaccgttt gataaccgtg
    18901 gcgaatggct tcccacgcat aaccgattgc ctggctgccg gaagtacagg cgctggaggt
    18961 tgggatcacc cgcccgcgca ggccgaaaaa gaggccagta ttaacggcgg tggtgtgcgg
    19021 catcatctgc acataggtgg tgccagtgat gttattggtg tgcttttccg tcagcatggt
    19081 ggcgaactcg cttaccgggc cggtactgcc ggtcgatgaa ccgtacgcaa tgcccgtttc
    19141 gccgctggtt aatatcggat cgccaatcaa ccctgcctgc tctaacgcca gctcgctggc
    19201 acgggtcgac atttgcgaca cgcggcccat cgcacggata cgcttgcggg tgtagtgctc
    19261 cggtagcgtg aaatcatcaa caggcgcgcc aagcagggtg tgcagcccat cgtagacctg
    19321 ccactccggc attttgcgca ccgcattttc atatgccagt aagcgtgcag aaacgtcctg
    19381 ccagttttca ccaaaggcag tcacgccgcc cattcctgta atcactacgc gacgtgtcat
    19441 aacatccctc cgttgatgga aataacctgg cgggtgacgt aacccgcaat atcagacatt
    19501 aaatagctgg caagcccggc aacttcttcc gcctggccca tgcgtttcat ggggatcatc
    19561 gacattgcct ctttcagggc gctctcttcc atttcgatca tcccggtatc aataagcccc
    19621 ggcgcgatgc agttgacggt gattttgcgc ttcgcgagtt cgatagccag cgcttttgtt
    19681 gcgccgataa ttccggcttt ggcagcgctg taattcacct ggccgcgatt gcccattacg
    19741 ccggaaaccg aggatagcgt gatgatgcgc ccaccctggc gtgcgccgat catcggcata
    19801 atgcacggct gaatgacgtt gtagaaactg tcgaggttgg tgtggatcac cgcatcccag
    19861 tcgtcatcgc ttaaagccgg aaaagcggca tcgcgggcga tcccggcgtt actgaccacg
    19921 ccgtaccagg caccgtgttg cgcgatttcg tgctccagca cttcccgaca ctgttcgcgg
    19981 tttgcgacgt cgaaactgag caaacgcccg ttacccccat tggcgacaat ggcattcagc
    20041 gtttcctgag cgcctgtggc gtcacggtga tagtgaacgc caatgttaaa gccgtctgcc
    20101 gccagttggc aggcgatggc gcgaccaatg cctttgctgg caccagtaac cagaactgaa
    20161 cgactcatca ggacgctcct tgttgaaaca gggtggtaag ttcttccgcg gtcggctgga
    20221 aggtgttaat gcggcccgtc gcctcgccgt cgttgatgct gcactcaaaa ctgccgaagc
    20281 gatcgtcttg catcagcagt ttgacggtaa tggtcagtgt ctggcctgcg ggcagaatgc
    20341 cagtggtgca aagcaattca cgcgcgccca gtatcatccc taactcaata ctgtttttgc
    20401 cctgctggtg gcgatgccag ccagaccaga cgccgacggt ttgcgccatc agttcaaggg
    20461 taaaccagcc ggggagattg ccgtccgggt cgagaaacgg tgccagtacg ccactgggcg
    20521 aaaccgtcac gcggcacacc gcgctgtcat cgctgacgct caccacgtct tccagcagta
    20581 acatgggcgc gtcgtggggc agataagcgc cgggggataa atagtggctc acgaaaccct
    20641 cccgagcaga atgctggcgt tattgccgcc aaaggcgaag gaattggaca ggatcaccgg
    20701 acgtgccagc ggctgcggtt tttcgataat gccacacggt ggcagcgtgg gatcgcgcgg
    20761 cgacaggctg aagtcctgag cgggcagcgg taaatcacgc tgtaaaatca acatactaat
    20821 tgccgcctcg gtaatgcccg ctgtgcccag cgtgtgtccg gtcaaatgtt tggtggagct
    20881 gcacggtacg cgttcgccaa acagggcgtt aaccaccatt gattcaatct gatcgttgag
    20941 ttgtgtggcg gtgccgtgca ggttgatata gccgacgtca tctggcgtaa gctgcgcatc
    21001 ggtcagcgcc tgattaatag cgcggatcgc tccttcgccc tgcggatgcg gggctgaaat
    21061 atggtaagcg tcgctcgatt caccaacgcc cagcaacgcg atgggctgcg gttcccgggt
    21121 cagcagcatt agccccgccc cttcaccaat ggtgatacct gcgcggtcac gaccaaacgg
    21181 ctgacataag gttggcgaca gcgattcgag gctgtgaaaa ccgttaatcg gcatccggct
    21241 cagggtgtct gcgccaccca caatggcggc atcgaccagc cccgcttcga taagcctgcg
    21301 cccgctgatt atcgctctgg cgctggaaga gcaggcggtt gaaagggtat aggccgggcc
    21361 gtcgagcgcc agccagtggc tgagaaaacg cgacggatcg ccgagttcct gttgcgcgta
    21421 gtgccatgtg gtgctcggct gcccgtgggt gcgcaggtta acgtgcgtgt cgccttcatg
    21481 caggccggag gtgctggtac caagcacaat cgcaatgcgc tcgcggccat atttcgcaat
    21541 ggcgtcatca acctgcggct ggatctgcgc cagcgccgcc agcaggatct gattgttgcg
    21601 cgagcggtgc gcagcgaatt tttccgggat aagcggaagc tcgccatcca cgccagccag
    21661 gaccgcctgc ggatgcccct gcaaccagcc tgcgcgcggg cgcatgccgg gagcaacacc
    21721 gcgagtcagg ttggttgcga tttcgtcaag gttatttccc agggcgttga tcatgccgac
    21781 ggcggaaata taaatcatat cagtcaccta agtattgaat ggtgatgtgg tatttaaaga
    21841 catgctgctc aatgctgatt ggcacgcgtt tgcccttgcg attcagatag gtgatttccg
    21901 tgaccagttt gccgctggcg ttacgcagct cgcgtttgtc accgttgtcg cgaagtgtcc
    21961 atcctgcggg aagttgcggt tgccaggcgc taatcggcca gtggctgagc atcacgtcag
    22021 ccagtacctg acttgcgggc ggtaactgtg ggacgacgat ggattgctcg gcgcgtagcc
    22081 cttgcgcatc gtaggtcacc agaaacaggc gaatgccgac cgacgacagc ccggcaaggg
    22141 tgattttctg atcgtcggcg ttaagcatca ctaacagcga ctgggttttg ccgttgaagc
    22201 tgccggtcaa cagttgctgc gaattgactg cgggtgaaat ccctggtgcc ggcagcgtga
    22261 tgcgcgtgcc aggttgcagc cacgcctgcg ggcggccttc ctgttctggt tgcgagtggc
    22321 tacagccggt gaggataagc gtagcggtca gggcgaacgc tcgccagaat gtggatttga
    22381 tcattttttt gttctctttt tatcgggcat agccagcggc gaaagcagga aggcggtgaa
    22441 aataccgctc accagcacaa tgccaaaact gctgatggcc tgggtggcgc tgaataccag
    22501 catacccagc gtcagcaagg tggtgagcat tgccagcgcg atcgccagta gcgaagttaa
    22561 cggtgtaccg cgcggattac tgaaaaacag cgtgtagttg ataccgatgc caagcaccag
    22621 caccagcgcc agcagcgaaa agagattcac cgcctgcccg ctcatcgcca gcaccgccag
    22681 accacagccc agcgaaagca ccgaaggcac caggctgata agccctttgc gccagccgag
    22741 acgggccact gcgccgcagg caatcactgc cagcgccacc agcaacaagc cggttaagac
    22801 gtagcggtaa agtgcgaaca attcatcaaa ggtgcttttg cgatcaaccc aggcaatgcc
    22861 gcaagggtaa tatgtggcga tttcctgcat caacgcgcta cttttaaccc cttcaaccgg
    22921 caccagtacg ccgctttcgc cgttttccag cgtcagccac agcagacgcc agccttcact
    22981 ggcggggctt gccagccatt catcaacgtt cactggcatg gcgttgagat ccgggttcac
    23041 tgccgtcagc ccggcatttt gcagcgcttt tgttactgtc ggggccgccg ttttcagcag
    23101 ttgtaaatct tcctcctgcc gcgcaaggga gttcagcgga atggtgcggt agttgctgat
    23161 aagcccctct tttttcgcat actcaagtga ggcggtatat ttctccagtc gccgcaatgt
    23221 ttgctgtggc gaatcgccgt aaaccacaaa ccatttttga tcgacgctct gcccggtcag
    23281 ggcggtaatg gctttttcct gcgccagaat atgctgcggt agcgcctgta actgcgagat
    23341 atcgtcatcg acgcgtagca ttgacatccc cgccagcgaa aacagcgcca gcgcgacggg
    23401 cagacccagc gacagttttt tattacgccg ccacgctgcc agccagcgta gcatcagcgc
    23461 catcgccgga accggacgca ccggcaggcc acggcacagc cacggatgcc agaacaggac
    23521 ggtcagacag gaggcgctca acccgacggc ggcaaaaatc gccatctggc gaatgccggg
    23581 gaagggggcg agcatcataa tcagatacgc cgccacggtg gtgagcagcg ccagtagcaa
    23641 ggcattgcgt actttcgcca ggctttgcca cggcgaaacg tcgttgccgt gaaccatccg
    23701 ctcggtgaga taatagagcg tgtagtcagc ggaaatgccg ataacgctca tgctcatcac
    23761 cagcgtcatc aggtgtaatt caccgaaaat caataaagtg gcgaccgttc ccgccagcgc
    23821 gccgatgccg atggaaatca cgcaaagcag caacgggcgt aaagagcgga acaccgccac
    23881 aatcagcaat atcaccccca gcagcgtagc cacgcccagg gtggagatat cctgcttcgc
    23941 ctgttggctg gcgtaatcgc tgtaaaacac cgtgccgcgc gagagcaact gtgcctgcgg
    24001 gtaacgcgtt ttcagatcct tttccagcgt attcagggtc gtgatcagct ggtgggtttg
    24061 ctgcatatca aacgacgatc ccgccagttc gccgtgcagc agataccagt agttgccctg
    24121 gggatcctgc gtcaccagcc aaccgtccat cagccgcaaa cgctggccgt ttttcgccat
    24181 tgccagctgc gagccgcgca ttaacatcag cggatcgttt tgcagctcct tgccgcttac
    24241 gccggagaat gcggaataaa gctgggagag gatccactgt gcctgcgctt cgccgccgtt
    24301 ttgcaggcgg gcgcgggtgt tggggtcaat caggccgttg cgatgctgcc agaaaaacgc
    24361 tccccacgcc tgctggctgg cggcatccat tggcccttta acgtcgccga gcgcagcgga
    24421 tttttgcagc agcgtcagcc actcctgagc gaccaaagga ttagcctctt taccggggct
    24481 gaccagccac accagttggc ggtcaagacg ctgcataaag ccgtcattca gcgccggagg
    24541 aatatcgccc atcgtctgtt tgggtaacat agccagcacg ctactgttca gccgtgattg
    24601 cggcagcagg atcagcaacg ccaccgccat gaccaggcag actagccccc ataacagcgc
    24661 ggggcgttta ctgggcggca aaacgttggc gttcgtcatc ggtcagttgc gctggcgtca
    24721 gttgatgttg ggtaagagca atatcggtgc gatcgccctg tttatcatta agttgaatgc
    24781 tctccagata ggttttcccg gcgagatcga tggtgttgaa aattttatcc agcggcgtgg
    24841 tggtcggcgt caggcgcagc gtccagcggc cttcgccttt gtcagcaaat tcgacgcgga
    24901 agttttgttc cagcactttg cgatcggcct ggaacaacgc gcgcagcagg tggttaaact
    24961 ggaacatctg cgggttgttt tctgccgtga tgatttgcgg cggctgaccg ttgatcacct
    25021 gcaccatgcg tttatcgtcc agtaatagtt gcatggggaa cggtgaggtt tgatcccaca
    25081 acagcccctg gtcgcgggcg atcaacatct ggccctgaga tcgcagcggc tgcggcagat
    25141 ctttaatcgt ccgggtttga tcgaaatggg cgcggatcac cggttgttcg gtaaagcgtt
    25201 gctgaagatc gtccagggtc agggcgctca caaacgggct aatcagcagc gccagcagcg
    25261 gtaaaaattt catggtttga ctcccatgcg ttcaaacaga atatcggggc tgacaaagca
    25321 cagttcgcga ctttgctcgt cgaccgcgac ctgaatggtg tatccggtgg tggcgcgttt
    25381 gcccgtttcg gcatcgaaaa tctgatagcc aatccgcaga cggttttcga actcttcgat
    25441 atgcgcacgc acgcggatgc gttgctcaaa ggtgagggcg tggcgatatt tcacccgcgc
    25501 atcgaccact ggccacaaat aaccggactc tttcatttgc cgatagccgt aattgaactg
    25561 gttgagcagc gcctcgcgag ccacttcaaa gtagcgaaaa tagttaccgt gccaggccac
    25621 gcccatcata tcgacgtcgt gaaagggaat ggtcagctcg acttctgcgg taaatcgggg
    25681 atcgttcagc accctttact ccttgtcctg aatttccggc agttgccaga aatcgaaaaa
    25741 attaaaccag tcgagaggcg actggagcgc gtaatgttcc agacgcgcgg cgtaatgatc
    25801 gatagcgttt tgcagcgcct gttggcgttc gccgcgcggc agctgcagtg ggtcggcaaa
    25861 gctttcgcag tgaatatgca gcttgccgtg ctggcgcagg gcgaaaatca gattcaccgg
    25921 gcagcgcaaa atagaggcga gaataaacgg cccctgtggg aaaggcgcag gctggcccat
    25981 aaacgaactc cagcagacgc gccagtcgcc gccgcgttgc gggttgacgg cgatgcggtc
    26041 accgacaatc gccacccatt cgccgttatc cagcttctct ttcagcagga tggcggtttc
    26101 tgggccgata tctgttaccg gcatcaggtt aatgcctgcc tgaggagcca tctcctgcat
    26161 tatctgttta aagcgttggg cgttttcgct aaacaccagc gcgttaatgg ttttgtagcc
    26221 ctgaattttt gccagcgccc ggcacacttc cacatcgcca agatgcgagg ccagcagcaa
    26281 tttgccgcgc ggatcgcgga cgtcaagcgc tgcttctgcg cctggcgcaa acagcacatc
    26341 acgccctggt tgtagctcgc cgcgccagct ggcgattttg tcgagcatgg cattaccgaa
    26401 acgtagaaag tgctgatagc tgttgagttt tgccgcctgt ggttgatgct gtcgtacacg
    26461 ggcgagccag tcttgcgacg ctttgcgcgc acgagaagca gtgagccagt agacgcccac
    26521 caccgggtaa agcagcgcgc taaacgccgt tcttcccagc agacgccaga ccagcagcat
    26581 caggcgcatt ccccataatc ctttcacttc actctgccgc gcccagtggc aggaagagcg
    26641 gcgcatcagc agtgaaggaa tacgcggcaa catgccgaaa aacagacgcg tgtgcatgag
    26701 cgagatgcgg acgttatctt tcagggcatc aaaatgcgaa agcccgtcca gtggataggt
    26761 gacgcgggtc ggcacgaaat agctggtatt tccctgccag tagaggcgca ccatcacttc
    26821 ggtgtcgaaa tccatccgct tgccgatggt ggcgtgtttt gccagttgca gcgttggcgc
    26881 aaccggataa acgcgaaaac cgcacatgct gtctttcagt tgcagggaga gcgtttcgat
    26941 ccatacccag acgtgggtga cccagcgccc gtaaagccgt gagcggggga tggagtcatc
    27001 gtaaattggc tggccggaga tcagtgccgc aggttgttgt tcagcgagag ccagcagttt
    27061 agggatatct tcaatcgcgt gctgaccgtc agcatccacc tgcaccgcat ggctgaaccc
    27121 tgcgtctgcc gctgcctgta agccacgcat caccgccgcg cctttgcctg cgttttctgc
    27181 caggcgaatt aaggtcacgc caggctgttc ggcaagcaga ctgtccagtt gctgttgtgt
    27241 ggtggcatcg ctgccgtcat ccaccacaat acagggcaga ttaaatggct taagacgcgc
    27301 cagcacgccc ggcatcatcg cgccgtggtt gtagcagggt atcaacacgc agggagaaaa
    27361 gtttaccgac ataggcggat cttcccgcta ctggcggtgt ggcgggcgtc accgtcgtga
    27421 cgctgatagc taaagctcag aatctggcgt tcttcctgcc agttaagcgc gagcgtgacg
    27481 cggttttccg gcagcaacgg agactggaat tttatgttct gaatggagtg aaaacgccag
    27541 ccaggcgcga gcagattgag cgcgtagctc atcgcccagt cgatttgcgc aactcctggg
    27601 agtaatggtt gcacggcaaa atgaccgcga aaccagaaca gatccgccct gagatgcaga
    27661 acaatttcaa ggtgattcgt ttgtgcctga tggcgctcga tttcatgggg cttcatgaaa
    27721 taactcctgt aattgcgcat agacacgctt gttcatactg tttactggca tttcatcaat
    27781 aacgcgccag taacgcggaa tagcaactgg ctccagcgtc gggcgcagta aacgccgcca
    27841 ggtcatttcc tggctatgcc cgccgccgtt ctgccattgc agatgagctt cgtcatccag
    27901 caccagcaaa acgcctatgc tctgacgccc gccgcgcgta acgggaactg ccgccgcctc
    27961 gcgaatgccg tccagcgcca gtagacgttg ttctacttcc tggagtgaaa tacgtttttc
    28021 ttcaatctta acgatgcgcc cacgacgccc catcagatgg aactgaccat cttcgctaaa
    28081 ctgtaagata tcgtcgagta acatgccgtt atcttccgct atcagcggtg aaaagaggcg
    28141 aaaggcatcg ccttcggcct ggaactgcat acctgggagg ggtagccagc ggcgttgctc
    28201 ttgttcgcgg tagcgccagg cgatgacgcc agtttccgtg ctgccgtaga tttcatcagg
    28261 ccagacgcgc agccagctcg cggtatgttg tacatcctgc cacggcagct caccgccagc
    28321 agagagaagc acctgaacgg gtggcggaga aagctgggta tccaggcgct tcaaaaatgc
    28381 cgggctgctg atgaagatat agcgatgctg atgactcaac gcggcaaact gttcgacata
    28441 ccagagcatg gcggcgtgca gcggtaatcc caacgccatg ggcaggaaaa cgcgaaaagt
    28501 caggccgtac aaatgctgcg gcaatacgga accgacgaca cgacaacccg ccagacgtgc
    28561 gcctaatcgt tcagccagca gttctgcttc acgatccagc aaatgaacag gtttaatcgc
    28621 ccgtttcggc tgtcccgtgg aaccggaggt aaacagctcg ataaaagcct ctggcgctat
    28681 agcggcaaag gtgaaggatt gtgtcgcgat ttgtggagaa cttgctacca acagcaatga
    28741 accttgccag ttaagttcgc tgtcgctaag cacaccatca aaaagttcac gttgctcgtt
    28801 aagctggata acacggttat gccccggaag caccggcgtt tttccggcgt gtagcgtggc
    28861 gagcagggca acgataaaca gatagctgtt ctcaaagcac agcgcccagc gttcacccgg
    28921 ctgttgctgc aagccgcaga taagttgcgc gacatcgtga cgcaaatcgc ccagcgtcca
    28981 ggtactttcg ttcagccacg caatgggtgt gtcatcaggg cgtggcgcag tcagccagcg
    29041 tgcaagcggt aaggtctgct tcatgcgcga tctcttttca tcacctgacg gcgcagcagc
    29101 cattcaccag ccattaacgt ccccatcaac aaataagcaa tcatgccgtt ccaggcggtc
    29161 cataacgaca tatcggcata aagcgcggta aagagggcaa ttccaccgtt aatgatgaaa
    29221 aagccgcacc agatttgcgt gacgtggcgg gtatagcgca cgcccttttc cgggaggtct
    29281 ggctcctgca accgcgccag ccgctcaact atcggcattg ccgaccacag cgaaccgcca
    29341 aaaaccgcca gcatgaccgc gttaaccact accgggtaaa acagcagtag ctggtgcgtt
    29401 ttcaacataa agctggcgac acataatgcg atgcccacga cagcgacgag ttgcgtgacg
    29461 gcttgtaaag gccccgcctg gcgacgggtc tggcgcagac gcaacaaaag cagcaacgcc
    29521 attagcggta acagccagtg caggccgtta tgcgctaagc caaaccaaat cacaaacggc
    29581 caggcgagca gcaataatcc agtcagcagc ttaatcatcg gtagcgagcg aatgcccgac
    29641 atcagatcag gcttcttgta gcaggcgttc tacagcctcg acgacatcct ggactgtacg
    29701 cactgcttta aactcttccg gcttgatttt cttaccggtt ttcttttgca ggtgcacaat
    29761 catgtcaacg gcgtcgatgc tgtccagttc cagatcttcg tacaggcgcg tttcaggttt
    29821 gatgtcctgc ggatcgattt caaacagctt aaccagcagg gctgagactt cctgatagac
    29881 ggtttgttgt tctgtcatga tggctttcct caggcacgtt gcgcagcaat gaaagaggcc
    29941 agagtggcga cggagaaaaa gtgctgacgc atctcttcgc tttccgcaga gagcacgatg
    30001 ccgtactcat tttttactgc cagtcccagt tccagggcat cgatagagtc cagccctaaa
    30061 ccgtcgccaa acagcggcgc gtcggtatcg atatcatcag gcgtcaactc gtccagattg
    30121 agggtgctga tgatgaggtt tttaatttcc agataaagcg cttgcattat ttattcctga
    30181 gagaggtaaa caaccttgtt gtaattgaag caaaagatgc cggtttaact gccttgctgc
    30241 cagcgccggt tcttgtgaag ttgcatcgta aaatttattg atttcaaccc gaccatggac
    30301 tgcaaccgta aaaaacggcc ggtcaggtgg aacgtcatac catttacttt gtttatccag
    30361 cagatgctcg ctacagccga ttgtcacgat gcgtaaatcg ctggcgcagc gcacggcaat
    30421 attcgccgcc ccgcgctgca gcgtcatttt ttcaccgggc ttcgttcgtg tgccttcggg
    30481 gaaaatcagg atagtgtcac cctgtgccag tcgctgttgg cagcggggta acagagtttc
    30541 agcttcgctg ttgattaaat aatctgtcgc ccgcacgacg ccgccaagaa aaggattttt
    30601 taacagcgcg cttttcacca ggcagtcagt ttctggcatg actgacgcca gcagcacgta
    30661 atcgatcagc gtgggatgat tggctacgac gaggcaaccg cgttcctgat gtaagatttc
    30721 agcgccatca atacgataat ccagtacgcc aagccctttc gcgacagtta aaaacaggcg
    30781 aaaactggcg gcaatgctgc gacgcgccaa acgacggcgg cgggaagtat cccacaccaa
    30841 aactaacaat atgttaaacc acacgacaga aagcagcagg ccgccgagac caaacagggc
    30901 aaaacataac ccggtcatga ccagtcgcca ggtccactcc agacgtgaca tcagcttact
    30961 catctgtggc tccagcgcca ttgcacgcgc tcgccaggca gtgaaaatgt gtctacattt
    31021 tgtaaatagt gctgtaaaaa caacatgctt tgcggcagtg ttgtttcatt gcccgcaatg
    31081 gctgactgcg tttcacattg ccagtcatcg ccagcttcaa tcaccagcgc gacggcgtac
    31141 ggccaggtcg gcatttctga tggcagttgt gggtgataaa actcgggcag gaaaccgtca
    31201 aaatcgacca tcagcacgcg ctggtatccc gcttgtagca ggctaagcac ttcacacaaa
    31261 ccttgctgga aactatcgcg accagccgaa agcgatgacg aaacgctcgg ctgtttggcc
    31321 gcaatggtca ggttacccac ggaagagttg tgtacggaga gcgcgaagtc cgtcggagaa
    31381 agcgcctgtt cggttgccag tgcatgaaca atgcggtaat tgcgctccag ttcgccatga
    31441 cgactggtat acaaaacggc gtctggttga tggcggcgta gcattgccag accgcattca
    31501 acagccagtt tgctcccgga gctgaggcgg cgggcggtca tcattggcag ttcacttaat
    31561 tttgccaacg gtgctgtcgg gtcaattgcc tgaggctgac gtgaccactc ctgccattgc
    31621 agcgtttcac taagtccagg cgctcttgcc tgccagtcga gaatgtttaa tgcaaatttc
    31681 atcaacgcgc gcccgcggta aaccaaaatt attaagtgac gattatttct tttggcatat
    31741 caaaagtgta tgcccaacgc ctaaattatc gtggcgctct gctacctgga atcctgcttt
    31801 attcagatag tcgtaaaatt tctctgcact atagaaacgg ctgttaccgt ttgccatgca
    31861 ggtaaaataa agtgaggacg cgttcaggct aaacgatgct gcttcaaacc gctgcgcatc
    31921 ccagaacaac tccatgatgc aaacttttgc gccaggcttc attacgctgg caattttgct
    31981 gagtatgcta attatttgtt cgggtgaaaa acagtctaaa aattggctca tccaccagat
    32041 atcagcctcg cccggtaatg gggcatcgct tagcatatcc accgcatgga aatctatgcg
    32101 atcagaaaaa cctgcattcg cgatattttc tttcgctagt acgatttgtt gtggcagatc
    32161 taataacgta acggcgatgt tttcattgta tttgcagcaa cgcaaagccc acttacccgt
    32221 attgccgcct acatcgtaca atgttgtggg gttgttggcg aatacataag gcagtgcggc
    32281 gttaaatgcg ccatctgaat agtaatgatc aaacgcaaac cagctttcac gagcggcgtc
    32341 tggtaattgc gaaagtgccg gatatatcgt cgaccagtcg ccaaatactt ttaacccgga
    32401 aggttttcct tcattgagtg aatcggcgag gaagaagagc ccctggtaac agacatcctg
    32461 ggtgaaatcc atattcacgc gagtcatagt gtcatgcagg aggtaatgac cgattttcgc
    32521 caggtaataa attccctctt tacaggtaat aattcgcccg cttaagccca tatctaacag
    32581 aacgctggta gcatattcgt tgatatggct atgttcggtg atatcagcca gcgttgcgcc
    32641 atgtttgcct tgccgatcca gatatgaaag tacacccgca ttacgtaggc agagtgctgt
    32701 ttgaaaaagc ataggggcaa aggcaatgcg ttgagcttcg gtaatcgcat ccagtgcgct
    32761 aagagaatcc tgttcgtaca tgttgggcac aacctgttaa gagggaatta aattattatc
    32821 ggaccgcaac ggcccgactt taggaagaat tataaatctg cgcccgctga caaattcagt
    32881 tgaatatctt tgtcgagatt gcggacccag cgattgtagt ttttatgaat ttgtccgcct
    32941 gacgcttgta ggttctggct attttcataa ttgatatcgt agctggatgc cgtgtagtta
    33001 atgcgtattt ccgctgaatg atcgcgtgac tgttgacgtg ctttaattat gccttgtccg
    33061 gcttcggaca taatccattt gcgttcaacg ccagctttca gaatcgcttt tttcacttgt
    33121 tcctgggtat gaccagcgct gacggttgag tgaacctgat caattggcgc agtacgcgcg
    33181 caacctgcta aagcacccac aacagcaata gccgcaaacc atttcatgta atgtctcatt
    33241 agaaccccat taaagtattg tttttacgag taaagcaagt tttaactgta attgctggca
    33301 tcactattct ttatttagag aaaaaattac ttgtgagata agtcagtaag tgcacatcag
    33361 agtaaaaatc tctgaaatga ctaaattact gccagcaggg accaactata agtgagtgag
    33421 aaaaatgagg caataaaaat aacacaatgt tatcagtgca ttctttgaga
//
LOCUS       DAZGXS010000038        33321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGXS010000038 DAZGXS010000000
VERSION     DAZGXS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(62..1243)
                     /locus_tag="ACHKG5_001969"
     CDS             complement(62..1243)
                     /locus_tag="ACHKG5_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0868080.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(1439..1533)
                     /locus_tag="ACHKG5_001970"
     tRNA            complement(1439..1533)
                     /locus_tag="ACHKG5_001970"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Sec,seq:tca)
     gene            1826..3208
                     /locus_tag="ACHKG5_001971"
     CDS             1826..3208
                     /locus_tag="ACHKG5_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0868081.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3218..5536
                     /gene="yicI"
                     /locus_tag="ACHKG5_001972"
     CDS             3218..5536
                     /gene="yicI"
                     /locus_tag="ACHKG5_001972"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0868082.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5589..7298)
                     /locus_tag="ACHKG5_001973"
     CDS             complement(5589..7298)
                     /locus_tag="ACHKG5_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0868083.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAELEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHKG5_001974"
     CDS             complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHKG5_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0868084.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     ISGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9090..10295
                     /gene="gltS"
                     /locus_tag="ACHKG5_001975"
     CDS             9090..10295
                     /gene="gltS"
                     /locus_tag="ACHKG5_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0868085.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10298..11164
                     /locus_tag="ACHKG5_001976"
     CDS             10298..11164
                     /locus_tag="ACHKG5_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868086.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLSVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHKG5_001977"
     CDS             complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHKG5_001977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0868087.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHKG5_001978"
     CDS             complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHKG5_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0868088.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHKG5_001979"
     CDS             complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHKG5_001979"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0868089.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHKG5_001980"
     CDS             complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHKG5_001980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0868090.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHKG5_001981"
     CDS             complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHKG5_001981"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0868091.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17270..18952
                     /gene="ligB"
                     /locus_tag="ACHKG5_001982"
     CDS             17270..18952
                     /gene="ligB"
                     /locus_tag="ACHKG5_001982"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0868092.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18949..19566)
                     /locus_tag="ACHKG5_001983"
     CDS             complement(18949..19566)
                     /locus_tag="ACHKG5_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0868093.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHKG5_001984"
     CDS             complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHKG5_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0868094.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20903..21766)
                     /locus_tag="ACHKG5_001985"
     CDS             complement(20903..21766)
                     /locus_tag="ACHKG5_001985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0868095.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21893..22609
                     /gene="rph"
                     /locus_tag="ACHKG5_001986"
     CDS             21893..22609
                     /gene="rph"
                     /locus_tag="ACHKG5_001986"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0868096.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22674..23315
                     /gene="pyrE"
                     /locus_tag="ACHKG5_001987"
     CDS             22674..23315
                     /gene="pyrE"
                     /locus_tag="ACHKG5_001987"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0868097.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23352..23948)
                     /gene="slmA"
                     /locus_tag="ACHKG5_001988"
     CDS             complement(23352..23948)
                     /gene="slmA"
                     /locus_tag="ACHKG5_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0868098.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24055..24513)
                     /gene="dut"
                     /locus_tag="ACHKG5_001989"
     CDS             complement(24055..24513)
                     /gene="dut"
                     /locus_tag="ACHKG5_001989"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0868099.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24491..25711)
                     /gene="coaBC"
                     /locus_tag="ACHKG5_001990"
     CDS             complement(24491..25711)
                     /gene="coaBC"
                     /locus_tag="ACHKG5_001990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0868100.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25883..26551
                     /gene="radC"
                     /locus_tag="ACHKG5_001991"
     CDS             25883..26551
                     /gene="radC"
                     /locus_tag="ACHKG5_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0868101.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26768..27004
                     /gene="rpmB"
                     /locus_tag="ACHKG5_001992"
     CDS             26768..27004
                     /gene="rpmB"
                     /locus_tag="ACHKG5_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0868102.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27025..27192
                     /gene="rpmG"
                     /locus_tag="ACHKG5_001993"
     CDS             27025..27192
                     /gene="rpmG"
                     /locus_tag="ACHKG5_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0868103.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27290..28099
                     /gene="mutM"
                     /locus_tag="ACHKG5_001994"
     CDS             27290..28099
                     /gene="mutM"
                     /locus_tag="ACHKG5_001994"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0868104.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28138..28617)
                     /gene="coaD"
                     /locus_tag="ACHKG5_001995"
     CDS             complement(28138..28617)
                     /gene="coaD"
                     /locus_tag="ACHKG5_001995"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0868105.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28625..29902)
                     /gene="waaA"
                     /locus_tag="ACHKG5_001996"
     CDS             complement(28625..29902)
                     /gene="waaA"
                     /locus_tag="ACHKG5_001996"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0868106.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30315..31373
                     /gene="rfaQ"
                     /locus_tag="ACHKG5_001997"
     CDS             30315..31373
                     /gene="rfaQ"
                     /locus_tag="ACHKG5_001997"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0868107.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31370..32494
                     /locus_tag="ACHKG5_001998"
     CDS             31370..32494
                     /locus_tag="ACHKG5_001998"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0868108.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32487..33284
                     /gene="rfaP"
                     /locus_tag="ACHKG5_001999"
     CDS             32487..33284
                     /gene="rfaP"
                     /locus_tag="ACHKG5_001999"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0868109.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7863 a         8286 c         8888 g         8284 t
ORIGIN      
        1 ccatccgcct gttggggctg gatggttatt cattattttt gtgtcaccga actttcattc
       61 atcaggccag ctcagaaagt gaatcaatgt agtcagcgta ccattgcatc atttcacggc
      121 gaccatcgag atactgggcg tggttatagg tacccctgat ggagttttta tccacatgag
      181 ccaactgcat ttcaatccag gcagaattaa agccttgttc gtgcagaatt gtgctcatgg
      241 tatgcctgaa accgtgccct gtgagtcttc ccttataccc cagcaattca atgacctggt
      301 taatgctctc cttactaatt ggcttgcgag gatcgttcct accgataaag accagaggat
      361 gatgttttga gattggttca agttgcttaa atagcgcaat aacctgctca gagggcggca
      421 caatatgagg ccgtttcatc ttcattactt ctgccgggat ctcccataat tttgcctcaa
      481 aatcaatatc ctcccaatgc gcgaaacgca actcttgcgt tcgtacaccg gttaacataa
      541 tgatctgagt agctgtctta gtgatgatac ttccggtata acccgccaga tcagtaagga
      601 agtggggaag ttcattggca gtaagaaagg gaaaatgtac ttttttcggt gtagctaaag
      661 cactggcaag atcgggagca gggttatagt cggctcgacc agtaacaatt gcatagcgaa
      721 acacctcacc acaacgttgg cggaccttgc gcattttctc cagtgctcca cgtttctcca
      781 tcttacgtag tgcttccaat agctccatcg gcttgatttc ggcaataggt cgcttgccga
      841 tgtaggggaa gatatctttc tcaaaggtgt cgattatttc atcgcggtag cgcagtgacc
      901 agcgatcggc tttcgtctga tgccactcgc gagcaacggc ctcgaaagtg tttttcaaac
      961 tcatttgcag cgctattttt tcttcttttt ttgcctcacc cggatcgcca ccagccgcaa
     1021 gtattttcct ggcttcactg cgtttctcac gtgcgtcggc aagcgttaca tcggggtata
     1081 cgccaataga cagcatcttt tctttaccgg cgaagcggta cttcatacgc cagtatttgg
     1141 aaccattagc gttaatcagc agatacatac ctccgccatc agccagctta tagggccttt
     1201 cttttggctt cgcggttttc actttaacat cggttagcgc catgacatcc tccagctttt
     1261 gatgaatcca tcctcctggg ggaatttccg ccagtaacca acctgatacc cccaatgcac
     1321 ccccacaaac aacttgatat gggttaaagt cgtttgacct caggatacaa agaaaacctg
     1381 atatataagg gattataagg ggctttgttg atgtcgggag agttcaggag aggcaaactg
     1441 gcggaagatc acaggagtcg aacctgcccg ggaccgctgg cggccccaac tggatttgaa
     1501 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
     1561 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
     1621 acacgcagcg tattatcacg aaagcattat aattcatata aaactcatat tttacaatga
     1681 tatatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
     1741 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
     1801 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
     1861 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
     1921 gatgttcttt tataccgata attttggcat acctgccgga tttgtcggaa ccatgttttt
     1981 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
     2041 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgtgt ggcgcactgc catttgggat
     2101 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     2161 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     2221 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     2281 tgtgctggcg accgcgggag gcatgctctc tactgttctg atgatgccac tggttaattt
     2341 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     2401 ggcattcatg atgctggcat tctgtttctt caccactaaa gaacgcgttg aagcaccacc
     2461 tacaacgacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     2521 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtccgcggtg gagcgatgat
     2581 gtattacgtc acatggattt tgggtacgcc ggaagtgttt gtcgcttttc tcaccactta
     2641 ttgcgtgggt aacctgattg gttccgcact ggcaaaaccg ctgaccgact ggaaatgtaa
     2701 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     2761 tgttcctatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtattgca
     2821 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     2881 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     2941 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     3001 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     3061 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     3121 gcacaatctg aaaaccgtta tggaacagct ggcccagggc aaacgccgtt gccagcaaca
     3181 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg acggaaactg
     3241 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     3301 gggtaatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgaa cctggcagct
     3361 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtacg
     3421 gattgagcat tttcaggggg cgctgaataa cggtcctcat tatccgctca atattttgca
     3481 ggacgtgaag gtcacaatcg aaaacacaga acgttacgct gagtttaaaa gtggcaactt
     3541 aagcgcgcgt gtcagcaaag gtgagttctg gtcactggat tttctgcgca acggcgaacg
     3601 tattaccggt agtcaggtga aaaataatgg ctacgtgcag gacacgaata atcaacgaaa
     3661 ttatatgttt gagcggctgg atcttggcgt tggcgaaaca gtttacggtc tgggagagcg
     3721 ctttactgcc ctggtgcgca atggccagac ggtagagacc tggaaccggg acggcggcac
     3781 aagtactgaa caggcgtata aaaatatccc gttctatatg actaaccgtg gctatggggt
     3841 actggtcaat catcctcaat gcgtctcttt tgaagtggga tcggagaaag tctccaaagt
     3901 gcagttcagc gttgagagtg aatatctcga atactttgtt atcgacggcc cgacgccgaa
     3961 agcggtactt gatcgttata cccgttttac tggtcgtccg gcgctgccgc ccgcgtggtc
     4021 cttcggcctg tggctaacca cttcatttac caccaactac gacgaagcga cggtaaacag
     4081 ctttatcgat ggtatggcgg gacgcaatct gccactgcat gtttttcact ttgactgttt
     4141 ctggatgaaa gccttccagt ggtgcgattt tgagtgggac ccgctgactt tcccggaccc
     4201 ggaagggatg atccgccgcc tgaaagcgaa agggctaaaa atctgcgtct ggattaaccc
     4261 ctatatcggg caaaaatccc ctgtctttaa agagttacaa gagaaaggct atttactcaa
     4321 acgcccggac ggttcgttat ggcagtggga taaatggcag ccaggtctgg cgatttatga
     4381 ctttaccaat ccggatgcct gcaaatggta cgccgacaaa ctgaaaggtc tggtcgcgat
     4441 gggcgttgat tgctttaaga ccgactttgg cgaacgtatt ccaatcgatg ttcagtggtt
     4501 tgacggttcc gatccgcaga aaatgcataa ccattatgcg tacatctaca acgaactggt
     4561 gtggaacgtg ctcaaggaca ccgttggcga ggaagaagcc gtcttgtttg ctcgctcggc
     4621 ctccgttggt gcgcagaaat tcccggtaca ctggggtggt gactgttacg ctaactacga
     4681 atcaatggcg gaaagcctgc gcggtggttt gtctattggc ctttcaggtt ttggtttctg
     4741 gagccacgat atcggcggct ttgagaatac cgctctggcg cacgtttaca aacgctggtg
     4801 cgcgtttggt ttgctctcca gccatagccg tttacatggc agcaaatctt atcgtgtgcc
     4861 gtgggcctac gatgatgagt cctgtgatgt ggtgcgcttc ttcacgcaac tgaaatgccg
     4921 catgatgccg tatctgtatc gtgaagctgc tcgtgcgaac gcgcggggta cgccgatgat
     4981 gcgggccatg atgatggagt tcccggacga tccggcttgt gattaccttg accgtcaata
     5041 catgttaggc gacaacgtga tggttgctcc ggtgttcagt gaagcgggcg atgtgcagtt
     5101 ctacttgccg gaaggtcgct ggacacatct gtggcacaac gatgaactcg atggtagtcg
     5161 ctggcataaa cagcagcaca gcttcctgag tctgcccgtt tatgtgcgtg ataacaccct
     5221 actggcgctg ggcaacaacg agcaacgtcc cgattacgcg tggcacgaag gcacggcatt
     5281 ccagctcttt aatctacaag acgggcatga agccatctgt gaagtgcccg ctgctgacgg
     5341 ttccgttctt ttcaccctga aagcggcgcg tactggcaac acgattactg tgactggtgc
     5401 gggcgaggcg aagaactgga cactgtgctt gcgcaatatt gtgaaagtaa atggtctgca
     5461 aggtggttcg caggctgaaa gtgagcaggg gctggtggtg acgcctcaag ggaatgcgct
     5521 gacaattacg ttgtaatcta ttgatggaat gccgggtacg tctgagtcgt acccggcgag
     5581 attgattttt actgcaccgg ctctgtcgat gtaacttcgc tactggaaac gacgcccgca
     5641 ttcatggttt gagtcacttg ctgctttgcg gcgttcacgg catggagttg cccgctaacc
     5701 gtaggtttca gcgggacatt ggcctgaata tcgccactgg cggtaagctg aatattgccg
     5761 tcgccagtca acggtaattc aggccatccc cattgttgca aaatattcac cggcacaccg
     5821 cgtccattca ggctgatatg ggtctgacgt tgtggcgttt gtgaaacact ggcagtggct
     5881 tccagaatgc ctttttcagt aaatgcactc agttcgctga tattcaccgt gctgctgttg
     5941 gcggtcagcg ccagcgacgg gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca
     6001 ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag cgtcaggttg
     6061 gcaccgtaac catcgagcgt ggtgagctgc cacgggaagt cagggtcgat atcaatgatc
     6121 agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt
     6181 tccatccaca actgttgcca gtttttcggc aaggtatatt ccagctcggc aattgccgca
     6241 tcatcaagga tcaacgtttt cccgtcacgc agccagttcc ctgacgttct gaccataccc
     6301 ccttcccagc ggctggtgaa ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt
     6361 ataatcgggt caaataaatg cagcgaacca taaatgaact cgctagcgtt catcgacagt
     6421 ttgccttctt gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg
     6481 aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatcacttc caggcgacca
     6541 atctgcaacg acgggacaga gcgtaatggc gcaaagaagt cggttagcga tttttcgctt
     6601 tgtagacgga tatccgccat gcgcagattt tccacttgcc agctgccgtc ggcgttacgc
     6661 tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg
     6721 cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc attgagcgaa
     6781 agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc ggcttctggt
     6841 gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc gctattcggg
     6901 ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag cggcgcggtc
     6961 tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg gcgtggttcg
     7021 gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt cgcgggctga
     7081 ccatcacgac caaacgtgac gttctccagc acgatatgag atggcgcgga aaaacggtga
     7141 tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc gctgatatgt
     7201 tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac taacagagcg
     7261 atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc cgtgaaatgc
     7321 acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt
     7381 gagactgcgg cggtaattat cttaccgccg ccagtgaatt actgtttttc tggtgggaaa
     7441 atcagattca gcacgatggc agtaataccg cccgcggcga tcccggagga gagcaggttt
     7501 ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc cagaccaacc
     7561 gccagcgaca gcgcgataat cagaatcgcc cgacggttca gcggctcacg agaaacgata
     7621 cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt
     7681 tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac gatcaacatc
     7741 agcgcgacga caaaaccgac atagcggctg gcaacaccag tcaactggat cactccgttg
     7801 ttttgcccga agcaggagtt cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg
     7861 ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga cactggctgt
     7921 tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt gatcataaag
     7981 accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata gagcggcgtt
     8041 ggcaccataa tcagttcttg cgtcatcggt tcgttgcttt ctggcaacat gcccataaac
     8101 cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg taagtaaggg
     8161 ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag cagcagattt
     8221 ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat ggacgttaac
     8281 ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaat cagcggcgta
     8341 ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtgca acttgccagc
     8401 atcaacgtgc cgaacaaagc cgccatcatg gtaggaacat cagcaccacc ggttttcagc
     8461 gcggtaccgc ccataatcag cggggcaaca aagttgaagc tggtgccctg aatagacaac
     8521 agcccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac accggaggca
     8581 aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag
     8641 attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcagatgctg acaggcggca
     8701 aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc gctgttttga
     8761 gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat cgaaaacaat
     8821 cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac gtttgccaca
     8881 tcctcatcta aatatttaaa tgagataaat gcactttttt atataacttt tggtattttt
     8941 ctgcttaaaa tccatgccat ttgttcatta acagctgaga atttgaccca ggaaagcata
     9001 actcgatacc cgtcatagtt catgaagttg ccactgcgcg atgaagtatg acgagtatga
     9061 aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta gcaacgcttg
     9121 ttgccgcaac gctgacgttg ttgctcgggc gtaagttggt ccattccgtc tcctttttga
     9181 agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg gcgctactag
     9241 tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc gatccgttaa
     9301 tgctggcttt cttcgccacc attggcctga acgccaacat tgccagtttg cgtgccggtg
     9361 ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg caaaatgcca
     9421 ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg ttggccggtt
     9481 ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg ttcattgaac
     9541 gttatggctt caccaatgcg acggaagtgg cgatggcctg tgcaacgttc ggtctggtgc
     9601 tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc accacgccga
     9661 acggtattcc ggatgaccag gaagtcccga cggcgtttga aaagccggat gtgggccgca
     9721 tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc ctgacggtgg
     9781 ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc ttcgtctgtg
     9841 tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt taccgcgtct
     9901 ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg gcgatggcgc
     9961 tgatggggct gaaactgtgg gagctggctt cgctggcgct gccgatgctg gcgattctgg
    10021 tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc atgatgggca
    10081 aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc ggtgcaacgc
    10141 caacggcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg cacatggcgt
    10201 ttttggtggt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg ctggtgatta
    10261 agctgtattt aatgttgccg atttttgccg gttaaaaatg aaaaagcgtc ggaaggaacc
    10321 ggaaacgcta cgtgaacact gtcgccatat ttttggtgat gagccgccag tgctcagtgt
    10381 atgggaaacg gaatttgatt acgcagatgc cgaactcaag gcgctggcgg ctaaagagtg
    10441 gcagcagatc agcgaatggg atttgagtgc ttactatgta ctcaatctcg tttataacga
    10501 accgatgcaa atagagttgt ttcgctatct gtttccgctc tgcctggcgc aatggcatga
    10561 aaccgtttta gctggcggtt atggtgatca cttcgaagaa tccttaatga aggcattgtg
    10621 tcggccctat ctctggcagg agatgatgaa tgcttcccaa cgccagcagg tacggcaatt
    10681 tttactggac accgcgttgc agcgtatgga taacgagcgt ggtttcaata acgttctttg
    10741 ctggctggcg gtgttcaata ctctgggtgg ggccgcgccg ttaattcgtt ctctctggtc
    10801 gcgctggtgg gcgctggata cgccaggaaa agcggtgtgt gctattcagt atgccgcgca
    10861 tctaatttat cccatagaag ccaatccgtt atggtcgcaa gaatggatag gctgggggca
    10921 tccattaggc cataaagatg gctggtcctc tgataatcgc gcttttcttc gccaaatgtt
    10981 aacacctgaa atgattgtgg ctggcgtaca ggcagcggct gaaattctgc gtggtgaacc
    11041 ggaaggcgca atggcggcgc gaattgcaca ggatgcgtat gaggcaatgg atatcctcac
    11101 tatccagatt gaagatttac tgcgtgactt gtcttgtgat gagtctggtc atgcgttgga
    11161 gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcttgtt gtgggtaacg
    11221 ttcgtgaata tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa
    11281 atccgccact ttaaactcag cattacccgt ctgacgcgta cctaacagtt cacccggtcc
    11341 gcgaatctct aagtctttct gtgcaatcac aaaaccgtcg ttgctgtcgc gcagcacttg
    11401 cagccgaatt tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaagc
    11461 caccgcacca cgacctacac gcccgcgaag ctggtgtaac tgcgccagac ccagacgctc
    11521 agggttttcg ataatcatca ggctggcgtt aggcacatca acaccgactt caataacggt
    11581 agtggcaact agcagatgta gctcaccttg tttaaacgac gccatcaccg cctgtttctc
    11641 ggctggtttc atccgcccgt gtaccaggcc aacattcaat tctggtagcg ccagtttcaa
    11701 ctcttcccag gtagcttccg ccgcctgcgc ttccagtaac tctgactctt caatcaacgt
    11761 acaaacccag tacgcctgac gaccttcagt tatgcaggcg tggcgcacgc ggtcaatgat
    11821 gtcggtacgg cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggcggcag
    11881 ctcatctatc accgaggtat cgagatcggc atacgccgtc attgccagcg tgcgggggat
    11941 cggcgtggcg gtcatgatca actgatgcgg atggaagcct tgctgctggc ctttctccca
    12001 taacgccaga cgctgatgca cgccaaagcg atgttgttcg tcgataatca ccagcgccag
    12061 accgttaaac tgcacctgtt cctggaagat ggcgtgcgta ccaacaatca tctgcacctg
    12121 accgctggcg atggcttcct gctgagacag tcgcgctttg cctttctgct taccggcgag
    12181 ccagcccact tcgataccga gcggttcaaa ccagttgcgg aagttattgg cgtgctgttc
    12241 ggcgagtaat tcggttggtg ccatcaatgc cacctgtttg ccgtgggcaa tcgcacgcag
    12301 cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgta ccaggcgcat
    12361 catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc
    12421 cgttggcttg aacggtaagg ctgcgaggag tttatttttc agcgcatcat tggcgctcag
    12481 cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga ggttgtgcgc
    12541 cagcagttct tccagaatca gacgacgttg cgctggatgc tgcccggttt ccagatcgct
    12601 aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta gcgtcatcat
    12661 tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga gcagatccag
    12721 cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg ttggataaac
    12781 cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc ggtattccgg
    12841 gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca gtacacggcg
    12901 gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca tggtgaggat
    12961 gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca
    13021 gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc cgatggggta
    13081 gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct gcacggtatg
    13141 caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta gggaactgag
    13201 tgggacagca tctaacaggc gacctgtcat ggcacttacc ctgcagcctg catagtagcc
    13261 caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata aggcaggcct
    13321 ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag gcgttgttgc
    13381 tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg ccgctgacgc
    13441 tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg
    13501 atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt gatgcccgtt
    13561 ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg gaaatcgaca
    13621 gcgttatcag aaagatgggt tgccagaatc tgcatgccct ggcctttgag atgagcgacg
    13681 gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc cgccgctgcc
    13741 gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg tacgccaacg
    13801 gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag
    13861 acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg
    13921 ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt
    13981 ttgcgcatga tattcgccag atgcacacgg tcacgagcgg tcagacgaat aaaggcgctg
    14041 tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta
    14101 attgccgccg tcaggtttgc cagcgcaccc tgatgattga acatctccac cttgatttcg
    14161 gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt ctctggctct
    14221 ttctggtagc cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg
    14281 ctgacgtggg cgataatcgg gtcgccagga atggggcggc agcatttcgc aaaggtgatc
    14341 agcacgccat cagcgccttt gatcggcaga tgcccgtggc tttgagtcgc cggtggaatg
    14401 gaggcgtccc catgttgtag atttttcgcg accaccacgc tcattgcgtt accaaggccg
    14461 atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc gcgctgaata
    14521 ttttcctgcg ggatttcatt gagcttacgg ctaccaccca aagcatggtt gagcagacga
    14581 cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg
    14641 cgcgctttcg agctaacgac aaagttcagc caagcggcat tcgggcgagc gcccggagcg
    14701 gtaatgattt caacggtctg accgctagta agcggctgcg acagcgggta aggctggcgg
    14761 tcgacgcgtg cgcccacgca ggcatggccg atatcggtat gcactgcata agcgaagtcg
    14821 acgggcgttg caccggcagg cagctcgaca atgcgccctt ccggtgtgaa aacgtaaatc
    14881 tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact accggcgctc
    14941 tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgtgc ggtagtactg
    15001 gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc
    15061 atatcttcgg tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg
    15121 tgcaacgact gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga
    15181 cgcggcttgt acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg
    15241 acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc
    15301 attttacagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc
    15361 gcttcctgca aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg
    15421 ccgcgcgcgg ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca
    15481 aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcggg
    15541 ctgtaaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc
    15601 agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg gatatcctgc
    15661 accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac
    15721 ttgagtttat caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc
    15781 tgttccatat cctggtaggt ggcgggagta tcttcaatca cgtcatgcag cagcgccgcc
    15841 atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc taccgggtgc
    15901 gtgatatagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga
    15961 tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat cagttgattc
    16021 aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata
    16081 gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg
    16141 tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt
    16201 ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga
    16261 cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca
    16321 gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga
    16381 aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg
    16441 ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag
    16501 tcatcattca caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc
    16561 gccatacgct ttgcaatgac ctcttcgctg tcctgatcgc gaccgcgtag acggcggtcc
    16621 agttcaattt tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga
    16681 atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact
    16741 tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg
    16801 aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca
    16861 ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca
    16921 tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg
    16981 gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa
    17041 taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac
    17101 actttttgcg caaagttgtt gcgttttgcg tagctgtttc cgcatttgcc tgtgatttac
    17161 tgcaataaag acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt
    17221 gccttcacca cgttgtatat ctgccagcct gactacagcg gaggtgaaga tgaaagtatg
    17281 gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg tctgtccggc
    17341 ctggtcgcca gccagagcac aggaagaaat tttccgcctg caacagcaaa taaaacagtg
    17401 ggacgatgac tactggaagg aaggaaaaag tgaggtggaa gacggtgttt acgatcagtt
    17461 aagcgcccgt cttacgcagt ggcaacgctg ctttgtgagc gagccccgcg acgtgatgat
    17521 gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc
    17581 ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg tgcagccaaa
    17641 agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag
    17701 tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa tttccgctgt
    17761 gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa tatttctcca
    17821 gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg
    17881 cctgatgatg cgccaggacg atagcgacac gctgaattct ctgggcgttt ttgtctgggc
    17941 atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta ccgcaggttt
    18001 tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaggttg cgcgcgtacg
    18061 caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg tgcgaggggc
    18121 gaaagaaccc gaatcccgcc attggctacc aggccaggca gaatggctgg tggcctggaa
    18181 atatcaacct gtagctcagg ttgccaaagt gaaggcgatt cagtttgcgg tgggtaagag
    18241 cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca
    18301 gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca
    18361 gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg
    18421 tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt
    18481 tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca
    18541 ggttctgggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg
    18601 ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg
    18661 gatcgcgaaa agtaaaagtg cgcagctatg gcatcggttt aatctggctc gtaagcagcc
    18721 ttttactcgc tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag
    18781 tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc agcagctgcc
    18841 ggggactgga tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa
    18901 gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgtccttc
    18961 gtggctgtag taaaagaccg gtaatcccag cttaaggcgt agcgccagta agcgggcaaa
    19021 aaagccgaat accagggtgg agataattac cacatcatgg ttagaaacat agtgttgcag
    19081 cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac
    19141 cagtgggata cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac
    19201 cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat
    19261 gatagaaaag accaccagtc caagcgcatc gagcaccaga aacactttgc gcaggtaagg
    19321 cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc
    19381 cgggtgtttg acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc
    19441 gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc ggcgtcgccc
    19501 ggccgccaga gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa
    19561 cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac
    19621 gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt
    19681 ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata
    19741 ggttaaaatt gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc
    19801 tggactttgg cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta
    19861 tttcttctct gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg
    19921 caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgctacccac
    19981 atcaaagtgt gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc
    20041 ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag
    20101 gatcttctga ctctttttca gccccttcag ttggtggatg gctttctgat ctaatccacc
    20161 atacagcccc tggtaaccat gattctgaaa tatggcgaaa tcagtagctg tcgctaccgc
    20221 agcttgctgt gctgcctcaa ccaattgttt gttatgttct ttcaactcat ttctcagaaa
    20281 cagacgcttt tcgtcttcac gaagttgctt aaatgcttca tcgtcagcaa gctcctgccg
    20341 tcgggtctgg atagcaaaat aagtctgccc tgccgcaatg accggtttcg cagggtcgcc
    20401 gttttgtact accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg
    20461 agcccctgaa cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg
    20521 attgctggct tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc
    20581 ccgataatcc agtaacggtg ccaattctct agctgaccaa aattcttgcc cttcagtgcc
    20641 ataatgccgt atttcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata
    20701 tcgattgcaa agaaagtaaa tctgtaactg tatttatata cagttgtgag tatctcacgt
    20761 tctcagtgtg ctgggcaaaa aaacatcaca aaatctggag caggcacgca aaacagaatt
    20821 gaagttgcgg ggaagaaaaa tgccgctctg tttgagtggg cggcatttgt gaaggctgaa
    20881 aagtaagaat tggcgttacg agttattcaa tgttctgaat ctgctcgcgc atctgctcaa
    20941 tcaacacttt cagctcaatc gcggagtttg tcacttcggc attgatagac ttcgacgcaa
    21001 gagtgttcga ctcgcggttg aattcctgca tcataaagtc cagacgacga ccaacggctt
    21061 cttttttctt cagaatgttg taggtctctt tgacatgcgc ttccaggcgg tccagttctt
    21121 cggcaacgtc aattcgttgt gccagcagaa ccagctcctg ctccagacgg ttgttttcca
    21181 actgaacctg agcatcttcc agcttcgcga ccagacgctc acgctgccat tgcaggattt
    21241 ccggcatatg ggcgcggact ttgaccactt cggcggtgac gccttccaga cgctgctcga
    21301 tcaatgcttt cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac
    21361 catccagcgc cgcgagaatt tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca
    21421 tcacgcccgg ccagcgtaga atatcaaccg ggttgatttc cccttcgtca ctctgcattt
    21481 ttacccagtt cgcggcagtt accagctgtt tagccagttt ttcgttgagg atcagctcac
    21541 cttgcgcgct aacatctggc tcatagcgca gggtacattc cactttaccg cgcgtcaggc
    21601 gagaacgaat acgctcgcga acgacaggtt caaggctacg gaactgctcc ggcagacgaa
    21661 agtaagtttc cagataacgc tggtttaccg agcgcatttc ccaggttgcg ctcccccatt
    21721 cacccttgat ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac gttcctgttt
    21781 ataaaaggag aggtggaagg attatagcca tcgatgcctt gtcaggatag gaataaccgc
    21841 cggaagtccg tataatgcgc agccacattt gtttcaagcc ggagatttca atatgcgtcc
    21901 agcaggccgt agcaataatc aggtgcgtcc cgttaccctg actcgtaact atacaaaaca
    21961 tgcagaaggc tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat
    22021 tgaagaaggc gtgccgcgct tcctgaaagg tcagggccag ggctggatca ccgcagagta
    22081 cggcatgctg ccacgttcta cccacacccg taacgctcgt gaagcggcga aaggtaagca
    22141 gggtggacgc acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga
    22201 tttgaaagcg ctgggtgagt tcaccattac gctggactgc gacgtgcttc aggctgatgg
    22261 tggcacgcgt accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa
    22321 gctggtggaa aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc
    22381 tgtcggaatt gtgaacggcg aagcggtttg cgatctggaa tacgttgaag actctgccgc
    22441 agagaccgac atgaacgtag tgatgaccga agacgggcgc atcattgaag tgcaggggac
    22501 ggcagaaggc gagccgttca cccatgaaga gctactcacc ttgttggctc tggcccgagg
    22561 gggaatcgaa tccattgtag cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg
    22621 actgatgagt cgcctttttt tgtctgtaga aaagtaagat gaggagcgaa ggcatgaaac
    22681 catatcagcg ccagtttatt gaatttgcgc ttggcaagca ggtgttaaag tttggcgagt
    22741 ttacgttgaa gtccgggcgc aaaagcccct atttcttcaa cgccgggctg tttaataccg
    22801 ggcgcgatct ggcactgtta ggccgttttt acgctgaagc gttggtggat tccggccttg
    22861 agttcgatct gctgtttggc cctgcttaca aagggatccc gattgccacc actaccgccg
    22921 tggcgctggc ggagcatcat gaccttgacc tgccgtactg ctttaaccgc aaagaggcaa
    22981 aagaccacgg tgaaggcggc aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg
    23041 tagatgatgt gatcaccgcc ggaacggcga ttcgcgaatc gatggagatt attcaggcta
    23101 atggcgcgac gcttgctggc gtgttgattt cgcttgatcg tcaggaacgg gggcgcggcg
    23161 agatttcggc gattcaggaa gttgagcgtg attacaactg caaagtgatc tctatcatca
    23221 ccctgaaaga cctgattgct tacctggaag agaagccgga aatggcggaa catctggcgg
    23281 cggttaaggc ctatcgcgaa gagtttggcg tttaaagaaa ctcgccggat gaaaagtcat
    23341 ccggcgtcat attactgcaa ctgtgccgca attagcggcc agcgggcgtc aaaatcatcc
    23401 gtcgggcggt atttaaattc gctgcggaca aaacgtgaca gcataccttc acagaaggcc
    23461 aggatctggc ttgccagcag ggtttcatcg gtggtgtaac cttcaccctc acgcattctc
    23521 ttttcacgca atacctgacg cagctgcgct tcaatacgct cgaacagctg gttgatgcgc
    23581 ccttgcaggc gatcctgttc aaacattagc gcatgaccag tgaggatgcg ggtcaggcca
    23641 ggattacgct caccaaaacc gagaagcagc aacacaatca gacgcaggcg cgctgtggtg
    23701 tctttctcat ctttcagaat cagattgatg cgagtaatca ggctatcttc gataaactca
    23761 atcaggctat cgaacatgcg ggtcttactg gggaagtggc gatacagtgc cgcttcggaa
    23821 acgccgacag aggcggccag ttttgccgtc gtgatacgtt ggcttccatc gctggattcc
    23881 agcatcagcg ccagagactg aagtatttcc tcgcgacggt tccttttcgc agtttgtttt
    23941 tctgccatgt tacaaaatac ccctgaaaat aagcacttgc caggcggcac ccacgctatg
    24001 accgcaaacg aaatgtttac ggctatgtta tgacgttatt cggatgcgta tgtgttactg
    24061 acgaccagag tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag
    24121 attaaattca gcctgtacta ccggaacaaa aatcatctgg gcgatacgtt cgccaggttg
    24181 aatggtgaag ctgtcctgac cacggttcca cacggaaatc atcaactggc cctgatagtc
    24241 ggaatcgatc aatcctacca ggttaccaag cacgataccg tgcttatgtc ccaatccgga
    24301 gcgcggcagc atcattgccg ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg
    24361 aaccagcgta gtgtcacccg gagccagttc tacggcgtcg tcgagacagg cacgcaggtc
    24421 aagtccggca gagccagagg tggcataagt cgggagcgga aattccttcc caacgcgcgg
    24481 gtccagaatc ttaacgtcga tttttttcat cataacgggt cacgatctcg tcgagtaata
    24541 attggccaag gagctctttg cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa
    24601 ggtgtaatgc gttgttgtcg ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc
    24661 agatcagatc aaggttttta cggatacgtt tttgccgggc gtattcttcc acattatttg
    24721 tttcggcggc aaatccaacg acgtagggtc gatggtcttt tagtgcggca acgcctgcga
    24781 cgatatcggg gtttttaacc atttttattg ttaattcatc accctgcgtg gcctgctttt
    24841 tgattttctc tggggccacg gtagctgcgc gataatccgc cacggcggcg cagccgataa
    24901 aaatattttg ctgctgtaca gaagcattca cggcggcttc catttccagc gcggtcatca
    24961 catcaacacg tttaacaaac ggtggcgtcg gtagtgaaac cggacctgat accagcgtga
    25021 cgttcgcgcc acgacgggca gcggcggcgg cgatagcaaa acccatcttg ccggagctgt
    25081 gattagagat ataacgcacg ggatcgagcg gttcacgcgt cgggccggcg gtaatcataa
    25141 tgttcagatg tttcaggtcg ttgacgggcg aaaaatgcgc taccgccata tccacaatgg
    25201 ttaacggatc gagcattcgc ccaggaccga tatcaccaca agcctgactg ccactgtctg
    25261 gcccccagat gagcaaacca cgggaagcaa gcacctctaa attatgctgc gtggcagcgg
    25321 cacggtacat ctgctggttc atggcgggga gcacggctac aggcgcaggt gtagccagac
    25381 aaatcgtcga taccaggtca ttcgccattc cggcagcaac acgggcaatc aaatctgccg
    25441 tggcaggggc gagaatcact aaatcagccc atttacccag ctcaatatgg cccatagcgg
    25501 cttcggctgc cgggtccagc aaactgtcgg aaacgggata accagaaacc gcctgcaagc
    25561 taagtggggt gataaaggct tttgccgctt cggtcatggc tacgcggacg tcggccccgc
    25621 gatcgcgcaa acgacgcacc agttcagggg ttttataggc agcaataccg ccgctaacgc
    25681 cgagaacgat ttttttaccg gccaggctca tgatgatttt tcctgttggg tgacaccaga
    25741 agttggcgat tttatcacaa tacttttgtt gtcgtgcctt cacctgagat tcactttgcg
    25801 aggcgctttc caggattgaa aactggccgt cgatttaacg gaacggctat gacaggatgc
    25861 gagcaccaca aaggaggtga aggtgaaaaa caatgcacag ctgttgatgc cgcgcgaaaa
    25921 aatgctgaag tttggtatta gcgccttaac tgatgtcgag ctgctggcgc tatttctgcg
    25981 taccggaacg cgcggtaaag atgtattaac cctggcaaaa gagatgctgg agaatttcgg
    26041 ctctctttat ggcttgttaa cctctgaata tgaacaattt agtggtgttc atggaattgg
    26101 cgtagcgaaa tttgcccagt taaaggggat tgctgaactg gcgcgacgtt actacaacgt
    26161 gcggatgcgt gaagaaagcc ctttactcag cccggagatg acgcgggaat ttttacaaag
    26221 ccagctcacg ggtgaggagc gggagatctt tatggtgatc tttctcgact cccaacaccg
    26281 ggttataacg catagccgtc ttttttccgg cacgctaaac catgttgaag tccatcctcg
    26341 ggaaattatc cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg cacataatca
    26401 cccttcgggt tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa
    26461 gagttgtcag ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta
    26521 tgtttctttt gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt
    26581 gtctgttcgg gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc
    26641 tcgggtttgg catttgggcc tggcaatcga gagttcacat agaactgcga tgaccgggct
    26701 gtaaagcctg acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag
    26761 aatagacatg tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg
    26821 ttcccacgca ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt
    26881 ctgggttgag agcgagaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt
    26941 aatcgataaa aaaggcatcg atacagttct ggctgaactg cgtgcccgtg gcgaaaagta
    27001 ctaagtactt agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt
    27061 tcttctgctg gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa
    27121 aaactggaac tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta caaagaagcg
    27181 aaaatcaaat aattctcgct ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt
    27241 tatctgcttg cccccatatt gactgcatct gttcattcct ggagatgcta tgcctgaatt
    27301 acccgaagtt gaaaccagcc gccgcggcat agaaccgcat ctcgttggtg caaccattct
    27361 tcatgcagtg gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgttt
    27421 aagcgaccaa ccagtgctta gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc
    27481 tgagggctgg attatcattc atttagggat gtctggcagc ctgcgcatcc ttccagaaga
    27541 acttccccct gaaaagcatg accatgtgga tttggtgatg agcaacggca aagtgctgcg
    27601 ctacaccgat ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg aagggcataa
    27661 tgtgctggcc catcttggac cggagccgct tagcgacgat ttcaatggtg agtatctgca
    27721 tcagaagtgc gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt
    27781 ggtaggggta gggaatatct atgccagcga atcactgttt gcggcgggga tccatccgga
    27841 tcggctggcg tcatcactgt cgctggcaga gtgtgaattg ttagctcggg tgattaaagc
    27901 ggtgttgctg cgttcgattg agcagggtgg tacaacgctg aaagattttc tgcaaagtga
    27961 tggtaaaccg ggctatttcg ctcaggaatt gcaggtttac gggcgaaaag gtgagccgtg
    28021 tcgggtgtgc ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg
    28081 tcggcagtgc cagaagtaat tcatgcgcgc cggatggcat accatccggc ataaacgcta
    28141 cgctaacttc gccatcagcg cctgatggac attctccggc aggaaatggg tgacatcgcc
    28201 ctgatggcgc gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg
    28261 catcagaaac acactttcca gttccggcat taagtggcga ttcatatgcg ccagctgcat
    28321 ttcatattca aaatctgcca ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt
    28381 acgggcgaag ttcgccatca aatcactaaa cccgaccact tccacgttcc ccagatgcgc
    28441 ggttgcctgc tgtgccagtg ccacacgctc ttccagggta aacatcggtt ttttactggg
    28501 gctggcggca atcgccagaa taacgtgatc gaacatctgc gtggcgcgcg tcacgatatc
    28561 gatatgacca ttggtaatgg gatcgaaagt acccggataa atcgcccgtt tttgcataac
    28621 aacctcaatg cgttttcggt ggcaggtaag gttccagcag ttgaagcaga cgctgtagcg
    28681 cgccctggtt ttgatacagt acttcaacgg catgacggcc atagaaacta cggtaatcgg
    28741 cgtcggtgag taaagaggaa acctcttttg caagcgtagt ggcatcggta acggtaatca
    28801 gcccgcttgc ctgctccagc cgcgcgcaaa tgtctttaaa gttaaaagta tgcggcccca
    28861 tcaataccgg aattgcgtgt gcggcagctt ccagcggatt atgcccacca cgttcaacca
    28921 gtgaaccgcc aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg
    28981 tatcgccaac cacaacctgc gtgctggtgg aggggacttc ccctgaagag cgtgtgatat
    29041 agcttagtcc agcctggcgg acaaggttaa tcgcatccgg gaagcgttct ggatgacggg
    29101 gtaccaggat gagcaataaa ttcgggaatt gctgtaataa agcctgatgt gcggcaatga
    29161 ccacgctctc ttcaccttcg tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc
    29221 actggcggcg cagcgtcacg gctttagcag ccaactgcgg cgttacagaa atatcgaatt
    29281 tcaggctacc ggtaacggtt acctggttat ttttcgcgcc cagcgccaca aaacgtgcac
    29341 catcttcttc attttgggcg gcaatcagcg taatacgacg cagcaagcga cggacgaatt
    29401 tacccagttt ggcataacct gcggccgagc gggcagagag tcgcgcgtta gcgatcacca
    29461 gcggaatttt acgtttatgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa
    29521 tcaacaccag tttagggtcg actttattca ggaaacggtt gagtgcatcg ggcagatcat
    29581 acggcagata aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg
    29641 ttggcgtcat ggttgttacg gtaataggta aatcaggata acgatgacgc agcgcgcgca
    29701 ccaacgggat cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg
    29761 gttttagcgg atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag
    29821 ccttacgtcc gcgcacccag agccgtatcc agatcagcgg ctgaataagg tagagaaggg
    29881 cggtgtaaag caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat
    29941 gtatttagct gtggctttac cattactttt cccgtttttg acttaaatag caatttaccc
    30001 gccaattctt gctgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
    30061 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
    30121 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
    30181 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
    30241 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
    30301 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
    30361 atggggatat gttattaact actcccgtca ttagttcgct gaaaaaaaat taccctgacg
    30421 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
    30481 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
    30541 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
    30601 atcaatggat ggttgctata ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg
    30661 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
    30721 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
    30781 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
    30841 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
    30901 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
    30961 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
    31021 cgcaaggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
    31081 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
    31141 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg tgcgacagac catattttct
    31201 ggcgtccttg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
    31261 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
    31321 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
    31381 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
    31441 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
    31501 atgccctgat gtatttgaac tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
    31561 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
    31621 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
    31681 cgagaaagtc gcgcaggaaa aaggcttttt ctatcacctg acgtcacgtt atcgccatta
    31741 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
    31801 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
    31861 tctaccaccg gggatttatc ctgatcgtaa atatagccag cagccagcta atagccgtga
    31921 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
    31981 agacttcacg cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt taccggattc
    32041 gctgcgccac aacacattgc tatatgttgt tggacaggat aaaccgcgaa aatttgaggc
    32101 gctggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
    32161 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcagcggg
    32221 aattgtgcta ctggaagcga ttactgcagg attaccggta ctaacaacag ccgtttgtgg
    32281 ctatgcgcat tatattgtcg acgccaattg cggcgaggct attgctgagc cattccgcca
    32341 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg
    32401 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
    32461 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
    32521 ggcgcggtaa agatcctttt gaggaagtta aaaccttgca gggtgaggtt tttcgtgaac
    32581 tggaaactcg ccgcactctg cgatttgaaa tggcgggcaa aagctatttt ctcaaatggc
    32641 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
    32701 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
    32761 tgtatggggt ggcatttggc gaaaaaggca tgaatccgct gaccagaact tcatttatta
    32821 ttaccgaaga tctgacacca accataagtc ttgaagatta ctgtgctgac tgggcgacta
    32881 accctccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcacg
    32941 atatgcatgc tgcgggcatt aaccaccgtg actgttatat ctgtcatttc ctgctgcact
    33001 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgacctg caccgggcgc
    33061 agcttcgcac gcgcgttcca cgtcgttggc gggataaaga tcttattggg ctttattttt
    33121 cttcgatgaa tatcggcctg actcagcggg atatctggcg gtttatgaaa gtgtattttg
    33181 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
    33241 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
    33301 gccaaaattt tggatacaga a
//
LOCUS       DAZGXS010000039        33056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGXS010000039 DAZGXS010000000
VERSION     DAZGXS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..542)
                     /locus_tag="ACHKG5_002000"
     CDS             complement(<1..542)
                     /locus_tag="ACHKG5_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868110.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQIYSGVSGG
                     ITYTIQYVEDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVYSDYCFDN
                     DENMSIQPEWINTPGISDLDS"
     gene            802..1722
                     /gene="cbpA"
                     /locus_tag="ACHKG5_002001"
     CDS             802..1722
                     /gene="cbpA"
                     /locus_tag="ACHKG5_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0868111.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1722..2027
                     /gene="cbpM"
                     /locus_tag="ACHKG5_002002"
     CDS             1722..2027
                     /gene="cbpM"
                     /locus_tag="ACHKG5_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0868112.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(2181..2780)
                     /gene="torD"
                     /locus_tag="ACHKG5_002003"
     CDS             complement(2181..2780)
                     /gene="torD"
                     /locus_tag="ACHKG5_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0868113.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2777..5323)
                     /gene="torA"
                     /locus_tag="ACHKG5_002004"
     CDS             complement(2777..5323)
                     /gene="torA"
                     /locus_tag="ACHKG5_002004"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0868114.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(5323..6495)
                     /gene="torC"
                     /locus_tag="ACHKG5_002005"
     CDS             complement(5323..6495)
                     /gene="torC"
                     /locus_tag="ACHKG5_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0868115.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6625..7317
                     /gene="torR"
                     /locus_tag="ACHKG5_002006"
     CDS             6625..7317
                     /gene="torR"
                     /locus_tag="ACHKG5_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0868116.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(7290..8318)
                     /gene="torT"
                     /locus_tag="ACHKG5_002007"
     CDS             complement(7290..8318)
                     /gene="torT"
                     /locus_tag="ACHKG5_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0868117.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            8401..11145
                     /gene="torS"
                     /locus_tag="ACHKG5_002008"
     CDS             8401..11145
                     /gene="torS"
                     /locus_tag="ACHKG5_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0868118.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            11217..12290
                     /gene="yccM"
                     /locus_tag="ACHKG5_002009"
     CDS             11217..12290
                     /gene="yccM"
                     /locus_tag="ACHKG5_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0868119.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(12339..12473)
                     /locus_tag="ACHKG5_002010"
     CDS             complement(12339..12473)
                     /locus_tag="ACHKG5_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0868120.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12501..12731)
                     /gene="ymcE"
                     /locus_tag="ACHKG5_002011"
     CDS             complement(12501..12731)
                     /gene="ymcE"
                     /locus_tag="ACHKG5_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0868121.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12706..12894)
                     /gene="ymcF"
                     /locus_tag="ACHKG5_002012"
     CDS             complement(12706..12894)
                     /gene="ymcF"
                     /locus_tag="ACHKG5_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0868122.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12905..13117)
                     /gene="cspG"
                     /locus_tag="ACHKG5_002013"
     CDS             complement(12905..13117)
                     /gene="cspG"
                     /locus_tag="ACHKG5_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0868123.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            13403..13615
                     /gene="cspH"
                     /locus_tag="ACHKG5_002014"
     CDS             13403..13615
                     /gene="cspH"
                     /locus_tag="ACHKG5_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0868124.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14057..14362
                     /locus_tag="ACHKG5_002015"
     CDS             14057..14362
                     /locus_tag="ACHKG5_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868125.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            14469..15113
                     /gene="gfcB"
                     /locus_tag="ACHKG5_002016"
     CDS             14469..15113
                     /gene="gfcB"
                     /locus_tag="ACHKG5_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0868126.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            15110..15856
                     /gene="gfcC"
                     /locus_tag="ACHKG5_002017"
     CDS             15110..15856
                     /gene="gfcC"
                     /locus_tag="ACHKG5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0868127.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15856..17952
                     /gene="gfcD"
                     /locus_tag="ACHKG5_002018"
     CDS             15856..17952
                     /gene="gfcD"
                     /locus_tag="ACHKG5_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0868128.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17998..19137
                     /gene="gfcE"
                     /locus_tag="ACHKG5_002019"
     CDS             17998..19137
                     /gene="gfcE"
                     /locus_tag="ACHKG5_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0868129.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            19125..19571
                     /gene="etp"
                     /locus_tag="ACHKG5_002020"
                     /gene_synonym="yccY"
     CDS             19125..19571
                     /gene="etp"
                     /locus_tag="ACHKG5_002020"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0868130.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19591..21771
                     /gene="etk"
                     /locus_tag="ACHKG5_002021"
     CDS             19591..21771
                     /gene="etk"
                     /locus_tag="ACHKG5_002021"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0868131.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21891..23195)
                     /gene="appA"
                     /locus_tag="ACHKG5_002022"
     CDS             complement(21891..23195)
                     /gene="appA"
                     /locus_tag="ACHKG5_002022"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0868132.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(23275..23367)
                     /gene="cbdX"
                     /locus_tag="ACHKG5_002023"
     CDS             complement(23275..23367)
                     /gene="cbdX"
                     /locus_tag="ACHKG5_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0868133.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(23380..24516)
                     /gene="appB"
                     /locus_tag="ACHKG5_002024"
     CDS             complement(23380..24516)
                     /gene="appB"
                     /locus_tag="ACHKG5_002024"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0868134.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(24528..26072)
                     /gene="appC"
                     /locus_tag="ACHKG5_002025"
     CDS             complement(24528..26072)
                     /gene="appC"
                     /locus_tag="ACHKG5_002025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0868135.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(26206..27063)
                     /gene="hyaF"
                     /locus_tag="ACHKG5_002026"
     CDS             complement(26206..27063)
                     /gene="hyaF"
                     /locus_tag="ACHKG5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0868136.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(27060..27458)
                     /gene="hyaE"
                     /locus_tag="ACHKG5_002027"
     CDS             complement(27060..27458)
                     /gene="hyaE"
                     /locus_tag="ACHKG5_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0868137.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(27455..28042)
                     /gene="hyaD"
                     /locus_tag="ACHKG5_002028"
     CDS             complement(27455..28042)
                     /gene="hyaD"
                     /locus_tag="ACHKG5_002028"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0868138.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(28039..28746)
                     /gene="hyaC"
                     /locus_tag="ACHKG5_002029"
     CDS             complement(28039..28746)
                     /gene="hyaC"
                     /locus_tag="ACHKG5_002029"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0868139.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28765..30558)
                     /gene="hyaB"
                     /locus_tag="ACHKG5_002030"
     CDS             complement(28765..30558)
                     /gene="hyaB"
                     /locus_tag="ACHKG5_002030"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0868140.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(30555..31673)
                     /gene="hyaA"
                     /locus_tag="ACHKG5_002031"
     CDS             complement(30555..31673)
                     /gene="hyaA"
                     /locus_tag="ACHKG5_002031"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0868141.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            32099..32186
                     /locus_tag="ACHKG5_002032"
     tRNA            32099..32186
                     /locus_tag="ACHKG5_002032"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32133..32135,aa:Ser,seq:tga)
     gene            32789..33056
                     /gene="tccP2"
                     /locus_tag="ACHKG5_002033"
                     /pseudo
     CDS             32789..33056
                     /gene="tccP2"
                     /locus_tag="ACHKG5_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT         8143 a         8680 c         8330 g         7903 t
ORIGIN      
        1 gaatctaaat cagatattcc cggcgtattt atccattctg gctggatact catattttca
       61 tcattatcaa aacaataatc agaataaaca tcatcacacg cattaatgtt ttcaatatat
      121 tccataaagc cataccaata ataaccttta aaatctgtga tagactctga acctctaccc
      181 ggcaaggata ctctaacaat atcaacatct tcaacatatt gtatagtata tgtaatacct
      241 cctgaaaccc ctgaataaat ttgtttatca ttagtacgca atgttttcat aaataatgat
      301 aaatcattat cattctgtat ttcctcaaat tctttttcta gtgaatcgca ttccaggcat
      361 ggaatgtagt cttcagcttg ttgacgtctg atttctttat acaaccgcat aaaacacacc
      421 agtgtgtttt caaaaagcgt gattgaatat gttttatttt tttcgtactc attttttata
      481 tcgttccagg cttgcttcaa ataattattt gcagtgcaac taattccatg tatattaccc
      541 atatatagcg cctataaaat ttaataaata ataacgcact agtaaaactt aaagtgcctg
      601 tttcaactat caaaaatcgc tcaccctttt tcacctgttt aaaatatgtt cagcgcccca
      661 ccttgaggcg acctcctctc cgcgatgatt tcaataacat attctgtgtt ggcatatgaa
      721 attttgagga ttaccctaca cttataggag ttaccttaca ggggttcctt caatttgtgt
      781 tgatttacgc gagataacgc tatggaatta aaggattatt acgccatcat gggcgtgaaa
      841 ccgacggacg atctcaagac aatcaagacc gcctatcgtc gacttgcccg caaataccat
      901 cctgatgtca gcaaagaacc ggatgccgaa gcccgcttca aagaggtcgc tgaagcctgg
      961 gaagtgttaa gtgatgaaca acgtcgcgct gagtatgatc agatgtggca acatcgcaac
     1021 gatccgcaat ttagccgtca gttccagcat ggcgacggtc agagttttaa cgccgaagat
     1081 tttgacgata tcttctcgtc aattttcggt cagcatgccc gccagagccg tcaacgcccc
     1141 gccgcacgcg gccacgatat tgaaatcgaa gtggcggtat tcctcgaaga aacgcttact
     1201 gagcataagc gtaccatcag ctataacctg ccggtttata acgcctttgg catgatcgaa
     1261 caggaaatcc cgaaaacgct gaatgtgaag atcccggcag gcgtcggcaa tggtcaacgc
     1321 atccgcctga aaggccaggg gacgccgggc gaaaacggcg gtccaaatgg cgatttgtgg
     1381 ctggtgattc atattgcgcc acatccgctg tttgatattg tcggccagga tctggaaatt
     1441 gtggtgccgg ttagcccgtg ggaagcggcg ctgggtgcta aagtcaccgt tccaacactg
     1501 aaagaaagca ttttgctgac tatcccgcct ggcagccagg ccgggcaacg attgcgcgtt
     1561 aaaggcaaag gtctggtgag caaaaaacag accggcgatc tgtatgcggt actgaaaatc
     1621 gtgatgccgc cgaaaccgga tgaaaacact gccgcgctgt ggcagcaact ggcagacgcc
     1681 cagtcgtctt ttgatccacg taaagattgg gggaaagcat aatggctaat gttacggtga
     1741 cttttactat taccgaattt tgcctgcata ccggcatctc tgaagaggaa ttgaatgaaa
     1801 ttgtcggttt gggggtggtt gaaccgcgtg agatccagga aacaacctgg gtatttgacg
     1861 accatgccgc catcgtggtg caacgcgcgg tgcgcctgcg tcatgaactg gctctggact
     1921 ggccggggat cgcggtggcg ctgacgttaa tggatgatat tgcgcacctg aagcaggaaa
     1981 accgcctgct gcgccagcgg ctttcccggt ttgtagctca tccgtgatgt gtgtttgccg
     2041 gatgtgatga cgcatcttag cctgcctgca aagagtactt ttcctgtggg ccggataagg
     2101 cgtgttcacg ccgcatccgg cggcggtgcc ctaatgcctg atgcgacgct aaacgcgtct
     2161 tatcaggcta acaaacgacg ttatctgttt tggtggtcgc cctcaactaa caccagcaat
     2221 aactggctta gtgccgcgta aaaaccaaag ctgtcatact gatggcaacg cgcaacaaac
     2281 tctggtaacc attgccgcaa cgccgtgagt gttttttgcc gtaaactgtc gattcttcgc
     2341 gcaggaaccg tcccctctcc cagcgaaaaa tgcagatgac tcaacaactc gagatagatc
     2401 gccagatgat ctgtcggttc gttgaaattg ccgctggttt ccatccctgc ctcaactaac
     2461 aagcgtttaa tctcttgctc gtcctgtttg taggccgatg catacggcag cgccgcttgt
     2521 ttgtcagtca tcagaaacag gccgcaaaag tcagcggcca gttccagacg ggcatcgtca
     2581 cgtactgtca gagtggcaac acggttttcc agctcatcca ccgccgcagt gagcggcggt
     2641 tcgcttttca gcaacgaaaa ccattcagcc atctgcgcac tggcgatttg cgtcagttgt
     2701 tcatcgtcca gttcacggga gaacaactgc gccagccagg cgtaaacaca ggctatctgt
     2761 tgtgctgtca gcgtggtcat aatttcacct gcgacgcggg aacatattcg cactgcgcca
     2821 ccatctccac ggggccgtta aacgccgtta cctgctccac tgttccgttg cacttctcaa
     2881 tttccaccag cgtagtgtgc gcactggtcg cctgcgccag ctgcgaagta ccgatgtcaa
     2941 tggtcaacac gttggggtta ccgtatttgc acagcgcacc aggctcgccg cctttatctg
     3001 gatcgtgcca tgccccttcg tgaattcgcg ccacgccggg tgcatagcgg tcagaaacta
     3061 ctgcccctgc caacacctga ccgcgagcgt taaagacgcg taccacatca ccgttacgaa
     3121 taccgcgcgc gctggcatcc tgcgggttaa tgaacactgg ctctttaccc gctaccgtat
     3181 attgctgacg cagagtttcc gactcacata actgcgagtg aagtcggaaa tccggatgca
     3241 cagattgcag atgcaacgga tacgtctgcg agccgggccc accgtgggag cgttcgattt
     3301 tctcaaacca catcggatgc ccctgacaat cgtcgtagtt catatcggcg atggttttcg
     3361 aatagatctc aatcaggcca ctcggcgtgc ccagcggttc gagatctgga tcttcgcgga
     3421 atgcctggtg gcgaacaaac atctgcggat ggtcaaactc gacgtactct ttgttattcc
     3481 agaagtcatc aaacgctggc aaatgaacgc cgcgtccttt gccttgctga acaccttcct
     3541 gccagatgcg tttcagccag cccatttcgt ccagcccttc ggtaaaggct tcttcgcgat
     3601 taaagcggcg gcacagctcg cggaaaatat caaagtcgtt gcgcgcctcg aactgcggcg
     3661 gcacgacctg tttcatggcg ataatgccac ggttggagtg gttgccgtac tggtcgagat
     3721 cgttacgctc aaactgcgtg gtcgcaggca gcacgatatc ggcaaagcgg caggttgagg
     3781 tccactggtt atctatggcg ataaccgttt ccagtttgcg ccagccttca ataatgcggt
     3841 tgatctgctg atggcgatgg aaggggttag ttccggcaaa aatacacatt ttcagcggcg
     3901 gcagttttac cgatttaccg ttccagttga ttactttccc cggttcgagg atcgcatcga
     3961 taaaacgggc aatcggaatg gtgctgctgt agcctttgta atcactgttg tcgtgaacag
     4021 gcggaatcga cgtagagccg gagaaaccac tcagaataac gcctttacgc cccggcgtgc
     4081 ctgcgccgtt atagtgccag ccaaaaccaa agccaccacc tggcaggcca atttgcccca
     4141 gcatcgccgc cagaaccaca atcatccacg cccactgttc accgtgctgc atacgctgta
     4201 cgcaccagcc agcaataatc tgcgttctgt tcgccgccat ctgccgcgcc agtccacgaa
     4261 tggtttcggc atcaatgccg gtcagttttt cagcccatgc ggcatctttc ggctgaccgt
     4321 ctttctcacc cagcagatac ggcaggaact gctcaaaacc cacacagtag ttagcgagga
     4381 agtttttgtc gtacaggttt tcactgtaca acgtatgcgc cagcgccagt tgcagcggca
     4441 cgtcagtttg cgggttaacc gcaatgtgct tcacatgctc gcgccccaga tactcatggg
     4501 tggatgtaac aaccggatcg atgctaatga cctcaatttc accggcggcg actttcgctt
     4561 tcaactgcgc gtaatattca taaacatcgt gatccgggca ccaccagttc gcttgctggt
     4621 ttttcagcaa atcggagccc cacagcacaa tggttttgct gttctgcaat accagcggcc
     4681 aggaggtttg ctgttcatac acttccatcg aaccgactac gcgcggcagg atcacctgcg
     4741 cagcaccagt agagtaatct ccgcccgtac caacgctatt accatgcaag gcaatagctt
     4801 tcgccagcat ccccgaagcg ttatggaaca tccccgtcga ttgccaacca ctggcggtca
     4861 gcaaggcact cggcccgtga gttttctgta cgcgttccag ttcttcatag aacatgtcga
     4921 gggcttcatc ccagctcaca cgcacaaaac ggttatcacc gcgctgggag gtgtcgctca
     4981 gatggcgctt acgcagccag tccacgcgta ccatcggata acgaatacgc gccgcgttgt
     5041 gtacatgatc cggcaatccg gcaatcattt tcgacggata tttatccagt tcgaacggtt
     5101 ttgccgccac aaagcgacca tccttcaccg tcgcgcggat agccccccag tgcgacccgg
     5161 taagaatgcc ctctttcgag atgacagcct cagtcgccgc ttgcgccgca gtcgcacggc
     5221 gcgacgttaa caatgacggc cccagcatcc cggcgacggt taagccgccg agttgtgcca
     5281 gaaaacgccg acgtgatgcc tgaaagagat cgttattgtt cattattttt cttccttctt
     5341 atcgccgtga gccttacctg cggtgtcaga cgcattcatt tgcagatatt tcaacaaggt
     5401 gcgttcttca cgtttatcga ggctggtaaa gccgatcatg ccgttgagcg tgccaatcca
     5461 gccgttagcg tcaaagtgag agatttccgg tgcgccgtgg cactggttac aggtgccgtt
     5521 gtacaacgaa tccgcataag cccagatcgg tttgatatcg ttcaccatgt cacctttctt
     5581 catccacgcg gtggcctgca acttgctcca ctcggtattg gtgtcggcaa cggtggtttt
     5641 ctccagcgtt tttacctgct gctgcacatc accacgaatc gaggcaacaa agatgcgttt
     5701 acctgggaat tgggtgagta cacgctgacg tccggcgctt tctgtccagc cggtaatttc
     5761 aatttgcagc cagtcgccgt cacgtttaag gactttcact tccgaggcag gcagcagaga
     5821 accagaggct tctttatcgc ctttcgccgc ataaattggc ttaatatcaa tagagtacag
     5881 cgtgtcacca ctgtcattag caccggcgcg cagctcatcg aactgcttac ggaagccgct
     5941 actcatatcc ggtaactggt gggcaatacc tttatgacag tcgatgcagg attgattatc
     6001 tttcgctgcc accttcatct gacgtgccgc ttcaggatgc tgcttcgcat gatccatcgc
     6061 atcgtagtta tggcaggagc gacaggttgc cgagttgttt tctttcattc gcgcccattc
     6121 acgctcggca agttccgcgc gtttggcttc aaatttttca ggtgtatcaa tggagtgggc
     6181 aataaaggtc tggtagatat cgttgctcgc ttccagtttg cgcttcacca tgcctggaat
     6241 atccggcggg atatgacagt catggcattc agctcgcacg ccggaggcgt tctggaaatg
     6301 caccgactgt ttatattctt catacactgg ttgcatactg tggcaactga cacaaaattc
     6361 ggttgtgctg gtgactttga tcccaacgtg tggcaataca atcagcgcaa tgccaatcac
     6421 aatcccaata gcgaccagcg ccagtaccga ccaacgagca ctgggtcggc gtagcgcgtt
     6481 ccagagtttc cgcataatag cccctgtaaa attatggttt agtgaagcga tcttaatgag
     6541 taaatatgaa cagcggcact ggtcaggatg aacggcttac ggcagaatat gaacagatat
     6601 gaacagaatg agtaaaaccc tctgatgcca catcacattg ttattgttga agatgagccg
     6661 gttacccagg cgcgattaca atcctacttc actcaggagg ggtataccgt ttccgttacg
     6721 gcgagcggtg ccgggctacg ggaaattatg cagaatcagc cggtggattt aattctgctg
     6781 gatatcaact tgcccgatga aaatggcctg atgttaaccc gcgccctgcg agaacgctca
     6841 acggtgggga ttattctggt taccggacgc agcgatcgga ttgaccgtat tgttgggctg
     6901 gaaatgggcg cagacgatta cgtcaccaaa ccgctggaac tgcgcgaact ggtagtacgg
     6961 gtaaaaaatc tgctctggcg aatcgacctc gcgcgacaag ctcaaccgta cactcaggac
     7021 aactgctatc gctttgcggg ttattgcctg aatgtgtcgc gccatacgct ggagcgggat
     7081 ggtgagccga ttaaactgac ccgcgcagag tatgaaatgt tggtggcatt tgtgaccaat
     7141 ccgggcgaaa ttctcagccg tgaacgtctg ctgcgtatgc tctctgcgcg tcgggtggaa
     7201 aaccctgacc tgcgcaccgt cgatgtgtta attcgtcgtt tacgccataa actcagcgcg
     7261 gatttactgg tgacgcaaca tggcgaaggt tatttcttag ccgctgatgt gtgctgataa
     7321 aaatagaccg gacgaaatcc cccaggcgac agcgagcggc ggatatgttc gcggtcggca
     7381 tttttcggcg tcagaactaa aatcggtggg ctgacattat cagacaccga ttgcccctgt
     7441 aattgcctga tggcctgctc aactgccagt tccccctgcc agaccatttg atcgctggca
     7501 gccataatca ctcttccccg cttcagcccg cgatacacct gatgtgaaag ataaaacgac
     7561 accacggtaa gcggcgtttt caggttacgc ccttccccca ttgccgcctc tgccgcaatg
     7621 gccgttccgg caacgacgtc gatttctgga tggcgttcca gcatctcctg caacaggtta
     7681 cgctggattt caatatcgtt atcaccaagc gcaatatcaa caatacgcac cgggcttccg
     7741 gcaatggctg cgcgaaaacc ctcgaccatc tccttactgc ccccggcgtt atcgggtccg
     7801 ggcatcaaca gcacattcag tggtttaccg tgcgcccatt gcaccagata tcgcccaggt
     7861 tgatagccca tctgaaacca gggcacacca acgcggcttt tcacctgggg agcatcaata
     7921 gcatttacca gctcgatcac cggcagattt gctacttgct tttgcagatc gggaaatgac
     7981 gtcgtgctac taccgagcaa aatggcctct gcgccccact gtttacactg gtcgatttgt
     8041 gcttgctggg tagccaactg gctgtagccg cctgcctcca gcacttttaa atccacaccg
     8101 tagcggcgag cagcctcctg cataccatag ttcaacgata accaatatga atctttcagg
     8161 ctgggataaa gcgcgcacag tttccatacg cgtttggctt ttagcggcat agaggcttgc
     8221 accgtgaaat gctgcgcatc atgccagcgc aacaggttat cagccgaaaa tgccggcaac
     8281 atgaaaaggg aaagaagtaa aaatagcagc acgcgcatga tagcctcatc aataataagg
     8341 ctttatgcta gatgcattcc gctttgcgac tcaacatttt tcaccttaag tgcaccgacc
     8401 gtgaatttaa ccctgacccg aagactctgg atgggctttg ccctgatggc gctgttaacc
     8461 ctgaccagta ccctggtggg atggtacaac ctgcgcttta tcagccaggt ggaaaaagac
     8521 aatactcagg cattgattcc taccatgaat atggcgcgcc agttgagcga agccagcgcc
     8581 tgggaacttt tcgccgcgca gaacctgacc agtgccgata acgaaaagat gtggcaggcg
     8641 caggggcgaa tgctcaccgc acaaagcctg aagattaatg cgttgctgca agcgttacgg
     8701 gaacaaggtt ttgataccac cgctattgaa caacaggagc aggagatctc ccgttcgtta
     8761 cgtcagcaag gggaactggt ggggcggcgt ctgcaactgc gccagcaaca acagcgactc
     8821 agtcagcaga tagtcgctgc cgccgatgag atcgcacgcc tggcgcaagg tcaggcgaat
     8881 aatgcggcaa cctccgccgg agcgacccag gccgggattt acgatttgat cgaacaagat
     8941 cagcgtcagg ctgctgaaag tgcactcgat cggctgattg atatcgatct tgagtatgtt
     9001 aaccagatga atgaactgcg ccttagcgct ctgcgggtgc agcaaatggt gatgaatctg
     9061 gggctggagc agatccagaa aaatgcgcca acgctggaaa agcagctcaa taatgcggtg
     9121 aaaattctgc aacgtcggca aatacgcatt gaagatccgg gagttcgtgc tcaggtcgca
     9181 acaacgttaa ctaccgttag ccaatatagc gatttgctgg cgctgttcca gcaggacagt
     9241 gaaatcagca atcacctaca aactctcgca caaaataaca tcgcccagtt cgcgcagttt
     9301 agcagcgaag tcagtcagct ggtcgacacc attgagctgc gtaatcagca cggactggcg
     9361 catctggaaa aagccagtgc acgcgggcaa tacagcctgt tattgctggg gatggtttca
     9421 ctttgcgcac tgattctgat cctctggcgc gtggtttatc gctcagtcac gcgtccactt
     9481 gccgaacaaa cgcaggcgct gcaacggctg ctggacggtg atattgactc ccctttcccg
     9541 gaaaccgctg gcgtacggga gctggacacc atcgggcggc tgatggatgc gtttcgcagc
     9601 agtgttcatg cactgaatcg ccaccgcgaa cagctggcgg cgcaggtcaa agcgcgtaca
     9661 gctgaattgc aggaactggt gatagaacac cgacaagcac gggcggaagc agaaaaagcc
     9721 agccaggcaa aatcggcgtt tctggcggcg atgagccatg agatccgcac accgctgtac
     9781 ggtattctcg gcaccgcaca gctactggca gataaccccg cacttaacgc ccagcgtgat
     9841 gatttgcggg caattaccga ctccggcgaa tcgttgctga ccatcctcaa cgatattctc
     9901 gattattccg ctatcgaagc aggtggcaag aatgtttcgg tcagcgatga accctttgaa
     9961 ccgcgaccgc tgctggaaag taccctgcaa ttaatgagtg gacgggtgaa aggtcgcccg
    10021 attcgcctgg caacggcaat tgccgacgat gtaccgactg cgttaatggg cgatccgcgg
    10081 cgtattcgtc aggtcattac caacctgttg agcaacgccc tgcgttttac tgacgaaggg
    10141 cagatcgttt tacgtagccg tactgatggt gagcaatggc tggtcgaagt ggaagacagc
    10201 ggctgcggta ttgatcccgc gaaactggca gaaatcttcc agccatttat tcaggtaagt
    10261 ggcaaacgcg gcggcaccgg gctggggctg actatcagta gccgtctggc ccaggcgatg
    10321 ggcggcgaac tgagcgccac cagcacgccg gaggttggaa gctgtttttg tttacgcttg
    10381 ccgttacgtg ttgccacggc acccgtgcca aaaacagtca atcaggcggt gcgtcttgac
    10441 ggtttacgtt tgctgttaat tgaagataac ccgctaaccc agcgaattac cgttgagatg
    10501 ctgaacacca gtggtgcgca ggttgttgct attggcaatg ccgcgcaggc tttagagaca
    10561 ctgcaaaata gcgaaccgtt tgctgccgca ctggtggatt ttgatctgcc ggatgtcaac
    10621 ggcattacgc ttgcccgaca actggcacga caatatccgt cgctggtttt gattggcttt
    10681 agcgcccatg tcattgacga aacgctgcgc cagcgtacca gttcactatt tcgcgggatt
    10741 atccctaaac cggtgccgcg tgaagtgctc ggtcagttac tggcgcacta tctccaactg
    10801 caagccaata acgatctacc actggatgta tcgcaactca atgaagacgc tcagttaatg
    10861 gggacggaga agatccacga atggctggca ttatttacac aacatgccct gccgcttctc
    10921 gatgaaatcg acattgcccg cgccactcag gatagcgaga aaataaagcg tgccgcgcat
    10981 cagctaaaaa gcagttgctc gagtctggga atgcgtagcg ccagccagct ctgcgcacaa
    11041 ctggagcagc agccattatc tgcccccctt ccacacgaag aaattacacg cagtgttgcc
    11101 gctctggaag catggttgaa taagaaagac ctgaacgcga tttgaatcaa tatcagccag
    11161 ttaagaataa ctagaatagt ggcatctggt atgcaactta acggactggc aaaatcatgg
    11221 cagagaaaaa aagaacccgc tggcagcggc ggccaggcac gacgggcggc aaattaccgt
    11281 ggaatgactg gcacaatgcc acgacctggc gtaaagcgac gcaattattg ctgttggcaa
    11341 tgaatattta tattgccatc acgttctggt attgggtgcg ctattacgaa acggcaggta
    11401 gtacgatatt tgtcgccaga ccgggaggta tcgaaggctg gctaccgatt gccggtctga
    11461 tgaatctgaa atatagcctt gcaacaggtc agttaccgtc cgtccacgcc gccgcgatgc
    11521 tgttattggt cgcttttatc gtcatcagtc tattactcaa aaaggccttt tgctcatggt
    11581 tatgcccggt tggtacgctt tctgaattaa tcggcgctct cggtaacaaa ctgtttggtc
    11641 ggcaatgtgt ccttccccgc tggctggata ttcctctgcg cggtgtaaag tatttgctgt
    11701 tgagtttttt tctctatatc gcgttattga tgcctgctca ggcgattcac tattttatgt
    11761 tgtcgcccta cagcgtggtg atggacgtta aaatgctcga tttctttcgt catatgggga
    11821 ccgcgacatt aatcagcgtg accgttttgc tgattgccag cctgtttatt cgccatgcct
    11881 ggtgtcgtta tctttgccca tatggcgcgc tgatgggcgt ggtttcgcta ttatcgccgt
    11941 ttaagattcg tcgcaatgcc gaaagttgta tcgattgtgg caaatgcgca aaaaattgcc
    12001 catcacggat cccggtcgat aaattaattc aggtacgaac agtggaatgt accggctgta
    12061 tgacttgcgt agagtcatgt ccggtagcct caacattgac cttttcactg caaaaacctg
    12121 cggcaaataa aaaagccttt gcgttgtctg gctggttaat gacgctactg gttctgggga
    12181 ttatgtttac ggtgattggt tacgcaatgt atgcaggagt atggcaaagc ccggtgccgg
    12241 aggaattgta ccgacgctta attccgcaag cgccaatgat tggtcactaa attagcaaca
    12301 agcggaacat cacctcgcac ggggtgatgt tcacaaaatc acattatttt gattttgaca
    12361 tcataacttt caagcccggt cattttttct cgcgcggtga agcgaatttc agagacttct
    12421 ttccctgtat tcttgttcag ctcggcgatt ttttgcgcga taaagtcggc gatttccgtt
    12481 tcggcttgtt ttttaactct tcaatattca ctgttaacct cttctggtct taaaagacag
    12541 caacacgcca gtaaggtgtt ggttcccggt accctggtca ggtaagaaat gttataagcc
    12601 atctccccga aagattcaat aatcaacagg ttatcgctta ctatacatac agcgttgaaa
    12661 aaaaataaga cagatataaa aaaggctcca caagggagcc tgatattatt ttgtgaaatc
    12721 cagcggtgca tggtcagtgc gtatttgcag cgcgacgtta tcaaaggtga tcatcgtcga
    12781 tttacaaaaa atacacttag cgcccaaagg gtttcgctcc gtcacatcga atgccgatgt
    12841 gcggtactgt gaaccatgac aacatggaca gcgaaaatgg agatgttgag taataacggc
    12901 aaccttagag cgtaactacg ttcgctgccg cggggccacg ttgaccctgc tcaatagaaa
    12961 attcaacttt ctgattttcg ttcagcgtgc ggaattcatt gctctggatg gcggtgaaat
    13021 ggacgaaaac gtctttgctg ccatcatcag gagtgataaa gccaaaacct ttatctgcgt
    13081 taaaccattt tactaaacca gtcattttat tagacatatt ttacttcctt cattttgggc
    13141 ctttcggcaa acagggcttt aatacagaaa ctacttagtg ttcaggagag agactcaaaa
    13201 gaaggggata atgcctgata atgagaactg ctttagtaaa ctactttgta tgctgtctgt
    13261 ctttccaacc gacgcagcta ttaacgcatg ataattgaga ttaagcaaat tttattttag
    13321 ccgtccggcg gtcttaaata tcgctggctt tattttatta accccgtata gtgcagcgca
    13381 gtattttcag taaggaattt gtttgtcccg taaaatgaca ggaattgtca aaacctttga
    13441 tcgcaaaagc ggcaaaggat tcattatccc ctccgacggt cgtaaagaag ttcaggtcca
    13501 tatttccgca ttcactcccc gcgacgcaga agtgcttata ccaggattac gcgtggaatt
    13561 ttgccgcgta aatggcctgc gaggaccaac agcggcaaat gtttatctct cttaatatga
    13621 cgctgcttgt ttaaagctga ctggttttat tggcatagtt caatagtcaa caccatgtat
    13681 tataacacaa gttacttttt caccggaaaa ctaatccatt taaccgaata tctctacaac
    13741 taatagttat aaatgccact taacagcgag ttatagtacc acttgaaact atattgccaa
    13801 caatagttaa tagctttctt aacaattttc ttatctttaa tttgtatttt gctaagattt
    13861 atcttatgct cacaggtcgt atttccagag gggaatagtt aacaatctta tatttccctc
    13921 tgcgcgcctt tctcacccgc tgtatatagt gcaagtggta tgaggaaagg gagatttcac
    13981 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
    14041 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
    14101 gacgccagct gcgtttgctg cccctgaagc agcaaacggg accgaggcaa cgacgggtac
    14161 tactggcacc acaacgacca ctaccggtgc aaccacgacg gctgctacca ctggtggcgt
    14221 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
    14281 gacgttggca gtagttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
    14341 caccacgtcc actacgcgct aattctgctt aaacgcgtcg aaattaaagt taaataatac
    14401 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
    14461 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
    14521 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
    14581 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
    14641 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
    14701 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaagacc gtcaaactta
    14761 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
    14821 taaaagacgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
    14881 ctgcgaccct gagttccact ttttcctttg ctggattaga gacgctgcat attgcgggtc
    14941 gcgatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgctacca gaaaaacagt
    15001 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
    15061 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
    15121 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
    15181 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccgtggagaa
    15241 tgttgcccag ttagtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgccct
    15301 gctgaccgac tccgcggtga aagctaaagc gcttaaagac taccaacatg tcatggcaca
    15361 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
    15421 ccagcagtta ctcaatctca atattactgg tcgcttaccg gtcaaactgg accctgattt
    15481 tgtacgagtg gatgagaaca gcaatccccc gctggtcggg aactatacgc tctataccgt
    15541 acagcgcccg gtgactatta ctcttctggg tgcggtttct ggggctgggc aattgccctg
    15601 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgtcttg caggcgcgga
    15661 caagaataac gttatcgtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
    15721 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttag gcttttctgc
    15781 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
    15841 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
    15901 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatcg
    15961 gactttggcg gcacaggatt gttgcagatg ccaaatgctc gcatcgcacc ggaaggtgaa
    16021 ttcagcgtca actaccggga taacgatcaa taccgtttct actccacctc cgtggcgctg
    16081 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgtaa atatagccag
    16141 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
    16201 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
    16261 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
    16321 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
    16381 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
    16441 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
    16501 aatcccctgc gtctgaaact cgaatacgac ggcaacaatt accagaatga tttcgctggc
    16561 aaactgcctc aggcaagcca tttcaacgtc ggtgcagttt atcgcgctgc cagctgggca
    16621 gacctcaacc tgagttatga acgcggtaac acgttgatgt ttggtttcac tttacggacc
    16681 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
    16741 cctgaatctg aaggattgca gtacaccacg gtagcaaacc aacttaccgc cctgaagtat
    16801 aacgcgggct ttgacgcgcc agaaattcag ctacgcgata agacgctgta tatgtctggt
    16861 cagcaataca aataccgtga ctctcgtgaa gcggtcgatc gtgccaaccg gattctggtg
    16921 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
    16981 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggcagg tacagcgcct
    17041 ggtcaatcag agccactgca acaacaacgt gttgaagctg aagatctttc tgcctttggt
    17101 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
    17161 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagcgcccgc
    17221 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
    17281 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
    17341 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaagc gaattacttt
    17401 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtat
    17461 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
    17521 gacgttaact acgtgaagca gcgtgactgg gacaacatga tgcgcttcac cgattattcc
    17581 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
    17641 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
    17701 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
    17761 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
    17821 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
    17881 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
    17941 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
    18001 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
    18061 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
    18121 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
    18181 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgcg
    18241 aactatgaat atcgtatcgg cattggtgat gtgttgatgg ttacggtctg ggatcacccg
    18301 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
    18361 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
    18421 acccaggtac gcaacgagat tacagcgcgt ctggattcag tcattgaaag cccgcaagtc
    18481 gatgtcagcg tcgcggcgtt ccgctcgcaa aaagcgtacg tcaccggtga agtcagcaaa
    18541 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
    18601 ggcggtctga ccgcagatgc agactggcgc aatgtggtgc tcacccagaa tggcgtaaag
    18661 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
    18721 cacccgggag atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
    18781 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
    18841 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
    18901 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
    18961 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
    19021 gtcactaccg cgcctctggc tcgttggaac cgtgtgattt ccctgctggt accgacgatt
    19081 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
    19141 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
    19201 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctggtaa
    19261 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
    19321 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
    19381 tggagtcgga acatattgct caggttaccg caatcgctcc cgaggtccgc gggaagacaa
    19441 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
    19501 atgcatttga acatgtctac ggaatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
    19561 tcagccggta atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
    19621 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
    19681 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
    19741 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
    19801 gccattctca gcggcctgag tgatatgatc cctaactcat cgcccgagtc tgcaccggag
    19861 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
    19921 gacatggttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
    19981 gagaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
    20041 cagcaactga cactcacggt tggggaaaac ggccactata cgctggaagg tgaagagttc
    20101 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
    20161 gatattaagg ccaaaccagg aacacagttt gtcctgagcc aacgtaccga actggaagcg
    20221 attaacgcgt tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
    20281 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
    20341 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
    20401 ttcttacagc gccagttgcc agaagtgcgc agcgagctgg accaggcgga agaaaaactc
    20461 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
    20521 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
    20581 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
    20641 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg ctatgccttc cacccaacag
    20701 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
    20761 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
    20821 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
    20881 ggttttattc ttggcctgtt tatttcggtg ggtgccgtgc tggcgcgtgc gatgttgcgt
    20941 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccaccatc
    21001 ccgatgtccg agtggctgga taaacgtacc cgtctgcgta agaaaaattt attttctaat
    21061 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
    21121 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagactgag
    21181 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
    21241 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
    21301 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
    21361 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
    21421 gtgattactc gcggtcaggt gccacctaac ccatctgaac tgctgatgcg cgatcggatg
    21481 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgttga tacgccgccg
    21541 atgctggcgg tgagcgatgc tgcggtcgtg gggcgctctg tcggcaccag cctgctggtt
    21601 gcgcgttttg gcttgaacac cgccaaagag gtgagcttgt caatgcagcg tctggaacag
    21661 gctggcgtta atattaaagg cgcaatcctc aatggtgtga ttaaacgcgc cagcaccgct
    21721 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaagg
    21781 taaagtgacc ggatgcggcg tgaacgcctt ttccggtcta cgcattagac agttcttcgt
    21841 ttgtcatcag cctcagagca ttcaggtagc tgaatgctct tttttataca ttacaaactg
    21901 cacgccggta tgcgtgcttc attcacgatt tgcgtaaaac cggccaacga acacatgccc
    21961 tgcgcatttc gctcttcaca tcctgccagg gtcagtttca cctctccggg cggcgtattt
    22021 aatgacagcg gagttttatc acgcatctgc tgtaaagtct ggaagaccag cgaaacctga
    22081 atccactggc tgttatcgct tagccgacgc cagcgttcaa acaccagttc accacctggc
    22141 ggcgtgttat ccggctgacc tggaagcgtc cagttgagct ccagtgcgcc gccgagattt
    22201 gccagattag tatcgtgtcc ggcgataaac agcactgaag tgggtaatgt cacaccatac
    22261 gcctgttttt gcggtggatg gggagtcaac gctatcatga tcaaatccaa caacggggtg
    22321 gcgcggctgc gggcaacctc tggcgtgcgt tgtagtaaat aaaattgcgc gttatgcaaa
    22381 cttagcaacg tgttccactg gtgtgaatca gtgatccttc cccaccccgg ctccggcatt
    22441 ccctgtgcct gttgcaggag aaatatttcc gtcagcattg atgcgaggct taccgcaccg
    22501 gttaatgaga cattgtcggc gctcaccttg agttccgatg gtaatgcctg cgttaatgaa
    22561 cagctttcgt cctgtttctc acggttaagg cacaagtttg attgcggaaa attaagcacc
    22621 cgttccagtt cgcgaaacgc cgtttgccga tgcccggtaa agtcagcaat tgaccctcct
    22681 gccctgctga ggatcgcgtc agtcacgttc gcgttatcca gttggcaaac gccagttttt
    22741 agaggattaa ataacggatc gggactggac gtatctgcct gggtatgtac ggttattgca
    22801 cagtcaggtg ccagcccggc ggcgaaggct tcgcctgttt tacgggtacg ctcgtcgaca
    22861 tcagcaataa tcgcgacctg accaggctgc gggcagccct ttttcgtcag caatccgtcg
    22921 gctaccagac gctggcgttg gtaatgtccg agataagcga ttagctcacc accgcgcggt
    22981 gtcagccaac ccagttttac cggccagttt ggccatgcgt ctggggtgac atcctgcatc
    23041 agttgcgtgg ccttggttgg ggcacgcaca ccatgacgac tgacaatcac cacactttcc
    23101 agcttcagct ccggctccgg ctcactctga gcgaatgcag attgcggggt taacggaatc
    23161 agaagagata aaaatgggat caagatcgct ttcatcgata tgtttccgct tttcatatcc
    23221 gacattattg attgcctgat acgacgctaa tgcgccgcat cagacttacg ttcgttaact
    23281 tttcaatcgc gggtccagcc ataccaacac aagggtggag agcgaacaca tcaacaaaat
    23341 gccgacgaac caaagtaaat accacattgt tcctgctcct tagtacagct cgttttcgtt
    23401 acggcggaga gtttctgtcg tcatgcgccc ccacattttg tagtagctcc agagagtgta
    23461 gagcaacaca atgggcaaaa atatcagcac gattaccaac ataatactca gcgtcagctg
    23521 actggaagtg ctgtcccaca acgtcaggct ggagattgga ctcacgcttg acggcatgac
    23581 aaaggggaac agcgtgatgc ctgccgtgaa aatcacgcca aattgcatca atgacgccat
    23641 caaaaatccc caacccgggc ggccacgata aatcgccatc accgtcagca atgggcagaa
    23701 gaaccccagc agcgggaaga tccacaaaac gggcgactcg acaaaattat tcatccaggc
    23761 accaggtagc actgccacca gtttcattaa cggattggaa ggaccgttag catcctgggc
    23821 gagcagtaca aagccatcaa taccgaccca cagccagtaa cccgccagca aaaagcacag
    23881 catcaccaac agtgcagcgc gtttagtcga cagctgtgaa cgcagatgaa tcacaccaac
    23941 agttttcagt tgtaaccaga cgccaccttg caaaatcacc atcccaaggc tgagcaatcc
    24001 gcacaataaa gggaatggcg tcagcagttg ccagaagctg ccgagatact ccacgcgtaa
    24061 ttgcggagtg aaggcaaacg gcacgccgag caacaagttg ccgaaggcga taccgaagac
    24121 taccggcggc accagactgc cgatgaccag accggcatcc cacattttac gccagcgtgc
    24181 atcggcgatt tttccgcgat aatcaaaggc cagcgggcgg aagaacagcg agcacagcac
    24241 caggatcatc gccacataaa agccggaaaa cgccgctgca tacactctgg gccaggcggc
    24301 aaataatgcc ccacctgcga ggatcaacca gacctgattg ccttcccagt gcgcaccaac
    24361 gctattaatc actatccggc gttcatcatc atcacgcgcc accagcggca gcaaacagcc
    24421 aatccccatg tcaaatccgt cggagatcat aaagaccacc aggatcacgc caatcagcag
    24481 ccaccagatg aagcgcaatg tttcataatc aaacatgatt ttctccttta cccctgttgc
    24541 tgcgtcggtt gttcactctg catcgcgctc ggccccagac gggcatattt ctgcatcagg
    24601 tagacttcgg cgattaagaa cagggtgtaa agccctacga tcatgatcag tgagaaagcc
    24661 agttgtcctg tggttaaagc ggagtgcgcg gagtatgtcg gtaagatgtc ctgtatcgcc
    24721 cacggctgac gaccaaactc ggtcataaac cacccggctt caatcgcaat ccacggcaac
    24781 ggcaaactcc agagcgccat tttcagcacc cagcgatgct ggtcgatttt gccacgcagc
    24841 gtctggacaa gcgcaatcag catcaccagt agcagcaggg aaccacagcc caccatgatg
    24901 cggaaactcc agaataccgg cgcaacctga ggtatcgcgc cacgcatcgc cgcctggtac
    24961 tgtgcggctg tgacatgatt catatccggc gcatagcggg agagcaacat gccgtagccc
    25021 aggtcgcctt ccagctcccg gaatgcctgc aacacatgcg gctcacgatt gccttgcgat
    25081 atttcctgca ttaacagcca ggccatacgt ccgcgttgca agcgtgggta ggtttcagcc
    25141 atcaaattct tcagacccgg cacgggttta tctaatgagt gagtggcgag gatccctaac
    25201 agcacgggaa ttttgatggc aaaggcgtta cgctcttgat cctgttccgg ccaggcaacc
    25261 acatggaacg gtgcaggagc aggttccgtt tgccactccc cttccatcgc cgccagtttt
    25321 accggttgta cttgcgcgac ttcatacgca gaactgtctc cgagttgcag ggtaccgata
    25381 atcgccagag taccgaagac ggaaccgatg gcaaacgagc gtaatgcgac atcgcgctcc
    25441 cgtccgcgca gtaaatacca ggcgctgatc gccataataa acatggcccc ggtcacgtag
    25501 cccgccatta cggtgtgcac aaatttcacc tggctgaccg ggttaaagac cagctcgctg
    25561 aagctggtca tctccatacg cagggtgtcg atatcaaaat gcgcaccggt cgggtattgc
    25621 atccaaccgt tggcattcaa tatccacaat gcagagagat ttgaaccgaa cgccaccagc
    25681 cacgtcacca gcaggtgctg gtatttattc agccgctgcc agccgaagaa gaaaagcccg
    25741 acaaaggtgg attcgaggaa gaaggccatt aatgcttcca tcgccagcgg tgcgccaaaa
    25801 atatcgccca catagttgga atagaatgac cagttagtac caaactgaaa ctccatggtc
    25861 aggccggtag ccacgccaag agcaaagttg ataccgaaga gcttacccca gaagcgcgtc
    25921 atatcgcggt agattgtttt gccggtgacc acgtaaatgg tttccatgat agccagcaaa
    25981 aaaatcagcc ccagggtaag gggtacaaat aaaaagtgat acagcgcggt cagagcaaac
    26041 tgccagcgcg ataaatcaat gacatcccac atgcgcactc ctgtaggcga agagagaaaa
    26101 ataacccgtt aatggtagat ctccctcttt atcctgaaac tctacgacca gaggtattcg
    26161 ccgattgcga tattcataag cctgaaagcg atacttaccg tctttttacg tcggtgcagc
    26221 ttccgccagc cactgacata cctcactaag ccgctgcgca gagtcgacca aatcttctgg
    26281 cgctgccagc accacttccg gtattgggca gatttcataa ctttccagta acgggccttt
    26341 taaggtgtcc gtacagcgta aatgccagac atggcgtaac cccgtggcgt tgatatagct
    26401 ctcgccatag ccaatggtac gaatctgaat atttcccggc ccacagagac gtgagagaaa
    26461 aagccgatca gcctcgctga tgggtaattg cgtcagatta atgctgtgcg gctgcgcgtc
    26521 ggggttacgt acatgcgcca gtaactcatg cgccaacggt aggccattca tcaggccatc
    26581 aatgggcggc ggtaacagcg aatctgtcgg caagagattt tgcgttgccg cctgccacaa
    26641 cgccagcggc gcgcagccag cctccagttt gtcctccagc aacttttcgc cgcgacgtct
    26701 gcgcacccgc cataatccgc agaagatcgc ctcctgtatt tcactttcgc tgtcgtcagc
    26761 ctgctgaatg cgcactgaca cttcaccttc cccgagaagc gtattgataa agtgacgatc
    26821 gtcggcgttg agatttaaca gatcgactgc cagcacctcg cccaacggcg gctggcgttg
    26881 acttagttga tgttgtaact cgtttaacag cgcaatcacc tgcgggctgt gagcacattg
    26941 ctccagggcc gccatactcg gttcatcatt cacctgacag gtgatcggca gtggattcat
    27001 gctgaaactg tcatcgttcg gttgcgttct tggccccagc agatggaaaa aagtttcgct
    27061 catgaggcac gctcctgctg tggttcgaca agcccacgca tcaggtttat cagttccgcc
    27121 cacgggtgaa taccattcag cacgccgcga tagtttccgc cggtaaacac taatgtggcg
    27181 ggaaagcgaa agacgccaaa gcgatctccg atggcttcgc tctgttcaag gtcagcaatc
    27241 gccacctgcc atgtgtagtc gggaaactcg cgcagtaatt cgccaatcat taccggatta
    27301 tcgctgacct ctggcgtgcg tttcgggtca ctgcttaata acaccacgcc gtctggcgct
    27361 tgcgtaagcc agtcgtcaag acggaattca ctaaccggcg tccagccgcg cgccagcatt
    27421 cgttgccaca acgcatcaaa tggcgtgtcg ttgctcatcc ctgctcctcc agtgtcatcc
    27481 tgtactggcg caagcgaacg ccttcgtaat tttccatcga cagacagtca taattgagac
    27541 agcgcgattc attagccggt tgcggcacaa ttccccatgc agccagctgc gccagcgccg
    27601 cctgttccgc tgcgggcagt tgctcccgtg ccagttcgct caggctgccg ccgtagtcgt
    27661 cgagcattgc gggttgcaga ccgacgaggg caatatgtgc tggcagatgt ccgcggatat
    27721 ctgccagcgc cagcacttcg gagaaactgt tctggtgcag gctcattttc ttcgcgctga
    27781 gataagccgg aatacgttct ccggcatagg ttcgcagcgt tcctggttcc agcccgtagt
    27841 caatggcatc gagaatcaac agatggctgg cgctttcgac ataccccagc aagttcagtc
    27901 cctgagtacc gccatcgaca atctccacag actcgggcca gtggtaatgg gcatacaacc
    27961 gttccgccac ccgcacgccg aaaccttcat cggcccacag caggttgccc agccccatta
    28021 ccaccacgcg ttgctcgctc atgaacgctc cttgttactt attttgccaa atttgtggct
    28081 acggtagccg ttgaccatgg tggagatcac cgtgtcgtcg gacatgatgt cttcacgcag
    28141 cgccatatag acatgaccga tcacaaacgc gccaatcagc cacatcccca gccgatgcca
    28201 gctgtgaatg tccattgagt tgcctcccgt ccagtagaaa aattccacca cataacggaa
    28261 cggcgcaaaa atagcgtact ggctgtgttc gctgtacagc gcaaagccgg tgatgatcat
    28321 aaagaccgac atcaggaaat agccaaacat cgccgcctgg gcgatggggt tatggcctat
    28381 atcggcactc ggacgttttg ccagaaacag ataccagcgg atttcatacc acacgccctg
    28441 ccaccagctt ttacgccata ctggcacgat aaacagctcg cgggagtatc gattgccaac
    28501 aaaagcccag tagatccgca tcagcaagac cacggtaaaa accatcccgg cgctgaagtg
    28561 aattaacctg atgtagccca tatagaacag atacgtcgcc tcgccgctga cggaaggtag
    28621 cggtttgccg ataaagtaac cggtgaccat caacaccgcc atgcataaca ccgtcagcca
    28681 gtgccagata cgcactggcg cttcaaagac atagtggctg acaacgttgt cgcttttctg
    28741 ttgcatgatg attctccttc gctgttaacg cacctgcacg gagatcagct cgctaccgtc
    28801 gtcgcccagc acgtgtgttg aacaggcgag gcacggatca aagctgtgca gagtacgcag
    28861 gatctccagc ggttgctcgg ggatcgccat tttggtgttc atcagcgccg cttcataagc
    28921 gccaatctgc cctttgggat cgcgcgggct ggcgttccag gtggtcggca ccacgcactg
    28981 gtagagatca atcttgccat cgcgaatggc ggcccagtgg cctaacgccc cgcgcggcgc
    29041 ttcggtaaaa ccgacaccac ggcactctgt cggccaggtt gtgggttccc atttttccgt
    29101 ggaggcggtg gcaagattgc cgtttttcag gttagtcatc agcttgttga agaaatactg
    29161 caacttacct gcggcccact gtgcttcgtg cgcgcggcac aaaatgcggc ccaacgttga
    29221 ctggataccg gaaagcggca ggttcagcgc cgacatcatg cgatcgaccg actcaacggt
    29281 cgcagcatcg cctttgtgat aagcgattaa cgtgcgtgcc agcggcccca cttccatcgc
    29341 gttaccgcgc cagcgcggcg ctttgatcca cgagtagcgt tcctgttcat tcagctgctg
    29401 aatgttggta tcgctgcctt tgacatcgcc ggggttgtac cacgggtcgg tgatgccatc
    29461 gaacggatga cgcccgacct gatcgttggg ataacgatac caggcgtgat cgacaaactc
    29521 ctgcacctgc tgcggatcaa ccaaatccac tggcagcaca ttgttgaagt cgccgttaat
    29581 caccgcgccg ccaggcatca gcagactttt ctcaccaaag tcgttggcaa tatccgggaa
    29641 tgcgccgtag ctgaggacac atttatcaga aagacccgtg ccgatttcgc tccacggttt
    29701 gttgaactga ccgatggcta aggcgtcggg gatcatcacg ttgttaatga agtctgccgt
    29761 gcgggtgata atcgactgca ccaggttcag gcgttccata ttgaccgccc cgaccgcgcc
    29821 gctttcgtca atgttaatgg cgcaaggcat cccgccgaca atccagtttg gatgcgggtt
    29881 tttaccgcca aagaccgcgt ggattttgac aatttcacgc tggaaatcga gagcttcgag
    29941 atagtgggca aagcccatca ggttggcttc tggcggcaac ttgtactgcg ggtgccccca
    30001 gtagccattg cggaagatcc ccaactgccc gccttcaacg aatttcttca ggcggttttg
    30061 tacgtcgaag aaatagccag gggatgattt cggccaagag gagagacttt gcgccagttc
    30121 ggaggttttc cgcgggtcgg ctttcagcgc atccaacaca tcgatccagt ccatcccggc
    30181 aagctgatag aagtgcacca gatgatcgtg gcaccagagc gttgccagca taatgttgcg
    30241 gatgatattg gcgttgtccg gcactttaat accgatggca tcttcgatgg cgtaaaccga
    30301 agccagagcg tgtacgccag tacagacgcc gcagatacgt tcaacgaacg cccacgcatc
    30361 gcgcgggtcg cgcccttgca ggatgatctc cagcccgcga aacatggtgc cgcaggagac
    30421 ggcattggtg attacattct gatcgttaat attcacttcg cagcgcatgt ggccttcaat
    30481 gcgcgtaatc gggtcgacca ccaggcggcg tccggcatta ttgatggtgt atccctgagt
    30541 ttcgtactga gtgctcatgc ctgtttatcc tcattgcctg gctgatgttc ggtttctgta
    30601 ggttgctgat tatgacgtct gcgctggtca acggcgctgg cgactgcgtg cacaccaaca
    30661 gccgctgcca ccacaccaag cgcggttaaa ccgacggtat cggcggtgga atgagtaccc
    30721 atttgtggaa tatcgaccac gcggctgtag aacgaaccgc gatcccagaa accattttcc
    30781 gcacagccca ggcagccgtg accagactgg attgggaaag aaacgccatc attccagcgt
    30841 gtggaggaac aggcgttata ggtggtaggc cctttgcagc ccattttgta caggcaataa
    30901 cctttgcggg cagcgtcatc atcccaactc tggacgaact ctccggcgtc gaagtgagcg
    30961 cggcgatagc atttatcgtg gatacgctga ccataaaaca tcagtggacg acccattctg
    31021 tcgacatctg gcaagcgatc aaaggtcacc atgtaagtaa tgatggcgct catcacatcc
    31081 gggatcggcg ggcagccagg tactttgata atgggtttgt cggtgatgac tttgtcgata
    31141 gacgttgcct gtgtcggatt aggtcgcgcg gcctgcacgc agccccagga cgcacaggtt
    31201 ccccaggcga taatcgcgct ggctccggca gcggcacgtt tgagtttctc aataaacggt
    31261 cgaccgctgc tgatacagaa catcccctgc tcgcccagcg gcggattacc ttctactgcg
    31321 aggatatatt tgccattgta ttgcgtgatg atgtcttcaa agacttcttc cgcctgggtt
    31381 ccggcggcag ccatcaaagt atcgtcgtaa tcgagggaaa tcagggaaag gatgacgtcc
    31441 tttgccagcg ggtgagcgga gcggataaaa gattcggtac agcaggtgca ttccagaccg
    31501 tggatccata ccaccggaat gcgcggtttg ttctccagcg cccaggcaat ctttggtgcc
    31561 attcccgcgc ctaatcccag cgacgtggca gccagactac aatatttgag aaagctgcgc
    31621 cgggtaacgc cctgacgccg catggcctgg taaaatgttt cctcgttatt catatcgcac
    31681 gtctcttctc cttgcgacac cggcaggaca ccgggcgctt atggtcggca agcgacgtcc
    31741 ctgtagcaag gcaaaccatc gcttcacgtg agatgctgaa aacgaaagct catccttctg
    31801 cactggcgca cgtcgccaga aagtattgtt aataaagcgt agtgaaactt ttgcacaaaa
    31861 caatacaaac tgtgtggatt tatcttttag cgataaaaat ggacctattt ttctttttgc
    31921 cgggcggtgg gatgtttagc cagttgctaa acgagtaaaa gagaaggaat ttatggtgat
    31981 gaaatgatca atctgctttt atttcacgct atcaatgcac ttttggctgt ttttcaggca
    32041 aacaaacaga ttaggggttt acacgccgca tcgggatgtt tatagtgcgc gtcattccgg
    32101 aagtgtggcc gagcggttga aggcaccggt cttgaaaacc ggcgacccga aagggttcca
    32161 gagttcgaat ctctgcgctt ccgccaaata atcaaggggt taccaaatgg tagccccttt
    32221 gcttttttgg ctcttggtat aaacttggta taccaattgg tatattttta ttcggatgag
    32281 attatcggtc atgagcggaa cctcaggttc ctcgtctgat gccgctcttg caacacgtta
    32341 cgctgccgag tatttttgta aaacatggac tgctccaggc ctgaaccagg cagaaggata
    32401 caaagccatc tcagatctgt cgcatcacta ttttcgtgca gaaggctcta gccctcccca
    32461 atcttttttg tgacaccctg tttcactgaa catgaagaca ctcaccttgt ccctgatatt
    32521 cattagcatc atcctgatta tttaacttct ctatttaggg aagcccgtct gatacaaagc
    32581 ttctattcct cgttaaatat tctccattct ggcgccaagc cctatctata gctgattgaa
    32641 aacaactcta aaaacgtaat aatatcaaat agtttattcc tacagccgat cattttgagt
    32701 aaaatattga cgagaatttc acaaaaggta gatttcatgt aaacggataa ataagactat
    32761 ccaataaagc tcttataagg ttatctatat gataaatagc attaattctt tttttccggc
    32821 cttcctcgca gtatatcaag tgctatacgc agcagtacct tcactgtctc gcagcacaaa
    32881 agcacaccta acacggtaaa aaccagctca cctttttctc ccagcaacag tcctgccagt
    32941 gcaacaacta tttttaaggt aaaaaattct tatacagaat caggacttca acgtcccaca
    33001 tcttatactc agtcaagtat agaaaaaaac gctttacatc ggcctctacc tgatgt
//
LOCUS       DAZGXS010000040        32200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGXS010000040 DAZGXS010000000
VERSION     DAZGXS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            37..336
                     /locus_tag="ACHKG5_002034"
     CDS             37..336
                     /locus_tag="ACHKG5_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868142.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(383..1270)
                     /gene="rarD"
                     /locus_tag="ACHKG5_002035"
     CDS             complement(383..1270)
                     /gene="rarD"
                     /locus_tag="ACHKG5_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP0868143.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1322..1789)
                     /gene="yigI"
                     /locus_tag="ACHKG5_002036"
     CDS             complement(1322..1789)
                     /gene="yigI"
                     /locus_tag="ACHKG5_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0868144.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1954..2823
                     /gene="pldA"
                     /locus_tag="ACHKG5_002037"
     CDS             1954..2823
                     /gene="pldA"
                     /locus_tag="ACHKG5_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0868145.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2956..4785
                     /gene="recQ"
                     /locus_tag="ACHKG5_002038"
     CDS             2956..4785
                     /gene="recQ"
                     /locus_tag="ACHKG5_002038"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0868146.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4849..5469
                     /gene="rhtC"
                     /locus_tag="ACHKG5_002039"
     CDS             4849..5469
                     /gene="rhtC"
                     /locus_tag="ACHKG5_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0868147.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5531..6151)
                     /gene="rhtB"
                     /locus_tag="ACHKG5_002040"
     CDS             complement(5531..6151)
                     /gene="rhtB"
                     /locus_tag="ACHKG5_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0868148.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6262..7284
                     /gene="pldB"
                     /locus_tag="ACHKG5_002041"
     CDS             6262..7284
                     /gene="pldB"
                     /locus_tag="ACHKG5_002041"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0868149.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7292..8092
                     /gene="yigL"
                     /locus_tag="ACHKG5_002042"
     CDS             7292..8092
                     /gene="yigL"
                     /locus_tag="ACHKG5_002042"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0868150.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8168..9067
                     /gene="bioP"
                     /locus_tag="ACHKG5_002043"
     CDS             8168..9067
                     /gene="bioP"
                     /locus_tag="ACHKG5_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0868151.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8955..9908)
                     /gene="metR"
                     /locus_tag="ACHKG5_002044"
     CDS             complement(8955..9908)
                     /gene="metR"
                     /locus_tag="ACHKG5_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0868152.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10144..12405
                     /gene="metE"
                     /locus_tag="ACHKG5_002045"
     CDS             10144..12405
                     /gene="metE"
                     /locus_tag="ACHKG5_002045"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0868153.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12445..13260)
                     /gene="ysgA"
                     /locus_tag="ACHKG5_002046"
     CDS             complement(12445..13260)
                     /gene="ysgA"
                     /locus_tag="ACHKG5_002046"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0868154.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13522..14283
                     /gene="udp"
                     /locus_tag="ACHKG5_002047"
     CDS             13522..14283
                     /gene="udp"
                     /locus_tag="ACHKG5_002047"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0868155.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14424..15851
                     /gene="rmuC"
                     /locus_tag="ACHKG5_002048"
     CDS             14424..15851
                     /gene="rmuC"
                     /locus_tag="ACHKG5_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0868156.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15946..16701
                     /gene="ubiE"
                     /locus_tag="ACHKG5_002049"
     CDS             15946..16701
                     /gene="ubiE"
                     /locus_tag="ACHKG5_002049"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0868157.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16715..17320
                     /gene="ubiJ"
                     /locus_tag="ACHKG5_002050"
     CDS             16715..17320
                     /gene="ubiJ"
                     /locus_tag="ACHKG5_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0868158.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17317..18957
                     /gene="ubiB"
                     /locus_tag="ACHKG5_002051"
     CDS             17317..18957
                     /gene="ubiB"
                     /locus_tag="ACHKG5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0868159.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19036..19305
                     /gene="tatA"
                     /locus_tag="ACHKG5_002052"
     CDS             19036..19305
                     /gene="tatA"
                     /locus_tag="ACHKG5_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0868160.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19309..19824
                     /gene="tatB"
                     /locus_tag="ACHKG5_002053"
     CDS             19309..19824
                     /gene="tatB"
                     /locus_tag="ACHKG5_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0868161.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19827..20603
                     /gene="tatC"
                     /locus_tag="ACHKG5_002054"
     CDS             19827..20603
                     /gene="tatC"
                     /locus_tag="ACHKG5_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0868162.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20645..21427
                     /gene="tatD"
                     /locus_tag="ACHKG5_002055"
     CDS             20645..21427
                     /gene="tatD"
                     /locus_tag="ACHKG5_002055"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0868163.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21424..21912)
                     /gene="rfaH"
                     /locus_tag="ACHKG5_002056"
     CDS             complement(21424..21912)
                     /gene="rfaH"
                     /locus_tag="ACHKG5_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0868164.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22079..23572
                     /gene="ubiD"
                     /locus_tag="ACHKG5_002057"
     CDS             22079..23572
                     /gene="ubiD"
                     /locus_tag="ACHKG5_002057"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0868165.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23618..24319
                     /gene="fre"
                     /locus_tag="ACHKG5_002058"
     CDS             23618..24319
                     /gene="fre"
                     /locus_tag="ACHKG5_002058"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0868166.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24511..25674)
                     /gene="fadA"
                     /locus_tag="ACHKG5_002059"
     CDS             complement(24511..25674)
                     /gene="fadA"
                     /locus_tag="ACHKG5_002059"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0868167.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25684..27873)
                     /gene="fadB"
                     /locus_tag="ACHKG5_002060"
     CDS             complement(25684..27873)
                     /gene="fadB"
                     /locus_tag="ACHKG5_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0868168.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28063..29394
                     /gene="pepQ"
                     /locus_tag="ACHKG5_002061"
     CDS             28063..29394
                     /gene="pepQ"
                     /locus_tag="ACHKG5_002061"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0868169.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29394..30008
                     /gene="yigZ"
                     /locus_tag="ACHKG5_002062"
     CDS             29394..30008
                     /gene="yigZ"
                     /locus_tag="ACHKG5_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0868170.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30047..31498
                     /gene="trkH"
                     /locus_tag="ACHKG5_002063"
     CDS             30047..31498
                     /gene="trkH"
                     /locus_tag="ACHKG5_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0868171.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31510..32055
                     /gene="hemG"
                     /locus_tag="ACHKG5_002064"
     CDS             31510..32055
                     /gene="hemG"
                     /locus_tag="ACHKG5_002064"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0868172.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7491 a         8148 c         8792 g         7769 t
ORIGIN      
        1 attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa cttttggcag
       61 ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc atggttattg
      121 tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgac attaaattta
      181 agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag atatacgatg
      241 tctctaggat ggagtaagta ctggggagtt ttggggggat ttcctgtaac aaatattgtt
      301 ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt aggctcttta
      361 aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc gcatccatca
      421 caaaaattgc cagcgccacc caaataaagg tgaaagtcac catcttatcg gcacccggtt
      481 tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg tactggaaaa
      541 agcctaacgt tgagagacga aggcgcgtgg cagcggcggt aaaacacaac agcggtacgg
      601 tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt tgccccatat
      661 ggctggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc agcagccaca
      721 tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc accaggccgt
      781 agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca aaagtccaca
      841 gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      901 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata aagtaaccaa
      961 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
     1021 caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
     1081 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
     1141 tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcaacttg aagtacgctg
     1201 gggctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
     1261 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
     1321 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
     1381 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1441 aacgctcgcc tctgcctggg cgcagataat cgacgcgaag atcaatggtc cccatccgcg
     1501 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccta
     1561 cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg tgcaaaatgc
     1621 tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt gcgaactctt
     1681 tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga taaacaaaca
     1741 tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata tcatccttac
     1801 acttcattgg ttaatgaaat gttgatttta tgattctttg ttgttggttt ctactttagg
     1861 aagggataac taacggcaat ggagttaagt atgtaaaata ccccgcagaa aaacattcac
     1921 cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg ctggttgttg
     1981 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     2041 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     2101 ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag cgatctgaac
     2161 aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga agtaaagttt
     2221 cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc ggtgttgggt
     2281 gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc ttcaccgttt
     2341 cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta ccgttttgca
     2401 ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg gcgttcagac
     2461 ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa cggtaactgg
     2521 ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc ggatatcacc
     2581 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtgctcagt
     2641 gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg cttaagttac
     2701 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg cgaatcgctc
     2761 atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa cgatttgttt
     2821 tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta ccctctcaga
     2881 gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt tttatttcag
     2941 gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg agctaaacag
     3001 gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga aattatcgac
     3061 actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg aaaatccctt
     3121 tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc accgctgatt
     3181 tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc ggcgtgcctt
     3241 aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg caccgggcaa
     3301 attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct tgagcatctg
     3361 gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc ccaatggggc
     3421 cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt cccgacgctg
     3481 ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat cgtgcgcctg
     3541 ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa tattcgctac
     3601 atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca ggaacagcgc
     3661 ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac cgctgcgcgc
     3721 ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa taatgtccgc
     3781 gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc gacggtggcg
     3841 ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga tattccgcgc
     3901 aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct gcctgcggaa
     3961 gcgatgttgt tttacgatcc agctgatatg gcgtggctgc gccgttgtct ggaagagaag
     4021 ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg cgcgtttgcc
     4081 gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg gcgtcaggag
     4141 ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg ttcaaccgat
     4201 gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat gggttatgtg
     4261 gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca tgacaaactg
     4321 aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt gatccgccag
     4381 ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct acaactgaca
     4441 gaggccgcgc gcccggtgct gcgcggcgaa tcctctttac aacttgccgt gccgcgtatc
     4501 gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga tcgcaaactg
     4561 ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt cccgccgtac
     4621 gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat caccgccagc
     4681 gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg taaaccgttt
     4741 atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc agcataaaaa
     4801 agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat gttgatgtta
     4861 tttctcaccg tcgccatggt gcacattgtg gcgcttatga gtcccggtcc cgatttcttt
     4921 tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg cgtgctgggt
     4981 attacctgcg gcgttatggt ttgggcaggg attgcgttgc ttggcctgca tttgattatc
     5041 gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta tctctgctgg
     5101 atgggttacc agatgctgcg cggcgcgctg aaaaaagagg cggtttctgc acctgcgcca
     5161 caggtcgagc tggcgaaaag tgggcgcagt ttcctgaaag gtttactgac caatctcgct
     5221 aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg tgataacgtt
     5281 ggcactaccg cgcgctgggg cattttcgcg ttgattattg tcgaaacgct ggcgtggttt
     5341 actgtggtgg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca acgtctggcg
     5401 aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca tttgattatt
     5461 tcgcggtaat gccagacgcg tcttcagagt aagtcggata aggcgtttac gccgcatccg
     5521 acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc ataaacaacg
     5581 agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat agagcaatcc
     5641 gttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata gtggtgacac
     5701 cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc ggaaatagcg
     5761 ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg cgctggaaca
     5821 aatgtcgacg cgattgagta gaggccagcg atttaagatc aattgcgcca gcggcgcgcc
     5881 attgctgtat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac acttcaaacg
     5941 caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca atatgaatcg
     6001 ccagtccggt ctgaagcccg gcaatagacg ccaccgcgcc gcgataaccg tggttgagcg
     6061 aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg attgatgtca
     6121 gtaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc tatttttgtg
     6181 ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg attttacgtg
     6241 gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac aagagaaaac
     6301 gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg tgatgaagca
     6361 gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc acagcaccat
     6421 gaccgggtgg tagtcatctg cccggggcgt attgagagtt acgtaaaata tgcggaactg
     6481 gcctacgatt tgttccattt ggggtttgat gtcttaatca tcgaccatcg cgggcaggga
     6541 cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt taatgattat
     6601 gttgatgatc tggcggcatt ctggcaacag gaggtccagc ccggtccgtg gcgtaaacgc
     6661 tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca acgccatcca
     6721 ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat tcgtatgccg
     6781 tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt ccgtgatggc
     6841 tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt actgacccac
     6901 agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac gattcgcgtc
     6961 ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca ggtgttagcc
     7021 ggtgcgggtg atgacgccac gccaacgctt ctcttgcagg ctgaagagga acgcgtggtg
     7081 gataaccgca ttcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca tcctgtcgaa
     7141 gggggacgac cgctggtaat taaaggtgcg taccatgaga tcctttttga aaaggacgca
     7201 atgcgctcag ttgcgctcca cgccatcgtt gattttttca acaggcataa ctcacccagc
     7261 ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt ctgatttaga
     7321 tggcacgtta ctttctcccg accatacatt atccccttac gccaaagaaa ccctgaagct
     7381 gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg ttgatgtggg
     7441 gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg gtgcgcgcgt
     7501 tcacgatctg gatggtaatc tgatttttgc tcataacctg gatcgcgaca ttgccagcga
     7561 tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc gcgacgacga
     7621 atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag cggtgttcaa
     7681 atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag tgttcttcac
     7741 ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc gttggggcga
     7801 tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg gcggcgtttc
     7861 aaaaggccat gcgctggaag cggtggcgaa gaaactgggc tacagcctga aggattgtat
     7921 tgcgtttggt gacgggatga acgatgccga aatgctgtcg atggcgggga aaggctgcat
     7981 tatgggcagt gcgcaccagc gtctgaaaga ccttcatccc gagctggaag tgattggtac
     8041 taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt aatcgttctt
     8101 tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa tcaaagagtt
     8161 ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct ttagctttta
     8221 tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc gcgttggcct
     8281 ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga aaacagtcgg
     8341 cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga gtttccgcgc
     8401 ttatctctac ctgacggttt ccgaactgct gctgttcacc gtactgacgc cgctctacat
     8461 cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggatatg cttttagtgc
     8521 cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg accatttctg
     8581 gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca tggtgggtta
     8641 caaacgcctg atggaaactc gcccgatgcc gcagcataac gcctttgcgt ggttctatct
     8701 tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc agaaaatgcc
     8761 gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt ccgggattgg
     8821 ctactttatg tggaactacg gcgcaaccca agtagacgcc ggaacgctgg gcattatgaa
     8881 taatatgcac gttccggcag ggctgctggt taacctggct atctggcacc aacagccgca
     8941 ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg tgcatcgtaa
     9001 gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt gcgcgctgag
     9061 cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc ggcgtacagt
     9121 cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg ctcaaaactc
     9181 tctactaccc aatgtggtag cgcggcaata cccatccgcg cggcaaccat ctgaatcaac
     9241 aataaggtgt tatcgacgct tttcagcgac gggctaacgc ctgcgggctg gagaaaatgc
     9301 cgccagacat ccagtcggct acgctgcacc ggataaatca acagcgtttc gctggcgaga
     9361 tcttccggcg tgattcgcgt tttcgccgcc agtggatggt caggtgctaa caccagacgc
     9421 acttcatagt cgaacatcgg cgaataatgc aggccactac gcggcagaat atcggaggtc
     9481 atcaccagat ccagctcgcc ctgctgcaac gcaggctgcg ggtcaaacgt tacgcccgat
     9541 ttaaaatcca tctccacctg cggccagttc ttatggaaat tttctaatgc gggcgtcagc
     9601 cactgaatac agctatggca ctcaatggca atgcgcaggc gcgtctgttg cggttcattg
     9661 caggcttgca gcgcctggct aatttgcggc agcacctggt ttgccagttg caacaggatt
     9721 tccccctgtg gagtaaagcg tagcggctgg ctcttacgca caaatagccg gaagccaagg
     9781 cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtttgatg caacgtcgcc
     9841 gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag gtgttttact
     9901 tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa tatacagtaa
     9961 ccgccaatta tggatgtgta aacatctgga cggctaaaat ccttcgtctt ttaaatttat
    10021 ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc cggggatgaa
    10081 taaacttgcc gccttcccta aattcaaaat ccataggatt tacatataat tagaggaaga
    10141 aaaatgacaa tattgaatca caccctcggt ttccctcgcg ttggcctgcg tcgcgagctg
    10201 aaaaaagcgc aagagagtta ttgggcgggg aactccacgc gtgaagaact gctggcggta
    10261 gggcgtgaat tgcgtgctcg tcactgggat caacaaaagc aagcgggtat cgacctgctg
    10321 ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtctgct gctgggtaac
    10381 gttccggcgc gtcatcagaa caaagatggt tcggtagata tcgacaccct gttccgtatt
    10441 ggtcgtggac gtgcgccgac tggcgaacct gcggcggcag cggaaatgac caaatggttt
    10501 aacaccaact atcactacat ggtgccggag ttcgttaaag gccaacagtt caaactgacc
    10561 tggacgcagc tgctggacga agtggacgag gcgctggcgc tgggccacaa ggtgaaacct
    10621 gtgctgctgg ggccgattac ctggctgtgg ctggggaaag tgaaaggtga acagtttgac
    10681 cgcctgagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc agaactggcg
    10741 aaacgtggca tcgagtgggt acagattgat gaacccgcgc tggtactgga actaccacag
    10801 gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt gaaactgctg
    10861 ctgaccacct attttgaagg cgtaacgcca aatctcgaca cgattactgc gctgcctgtt
    10921 caggggctgc atgtggacct tgtacatggt aaagatgacg ttgttgaact gcacaagcgc
    10981 ctgccttctg actggctgct gtctgcgggt ctgatcaatg gtcgtaacgt ctggcgcgcc
    11041 gatcttactg agaaatatgt gcaaattaag gacattgtcg gcaaacgtga tttgtgggtg
    11101 gcatcttcct gctcgttgct gcacagcccc atcgacctga gcgtggaaac gcgtcttgat
    11161 gcagaagtga aaagctggtt tgccttcgcc ctacaaaaat gccatgaact ggcactgctg
    11221 cgcgatgcgc tgaacagtgg tgacacggca gctctggcag agtggagcgc cccaattcag
    11281 gcgcgtcgtc actctactcg tgtacataat ccggcggtag aaaagcgtct ggcggcgatc
    11341 accgcccagg acagccagcg tgcgaatgtc tatgaagtgc gtgctgaagc ccagcgtgcg
    11401 cgttttaaac tgccagcgtg gccgaccacc acgattggtt ccttcccgca aaccacggaa
    11461 attcgtaccc tgcgtctgga tttcaaaaag ggcaatctcg acgccaataa ctaccgcacg
    11521 ggcattgcgg aacatatcag gcaggccatt gttgaacagg aacgtttggg actggatgtg
    11581 ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga gcacctcgac
    11641 gggtttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg cgtgaagcca
    11701 ccgattgtta ttggtgacgt tagtcgcccg gcaccgatta ccgtggagtg ggcgaagtat
    11761 gcgcaatcta tgactgataa accggtgaaa gggatgctga ctggcccggt gaccattctc
    11821 tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca aattgcgctg
    11881 gcgctgcgtg atgaagtggc ggacctggaa gccgcaggaa ttggcatcat ccagattgat
    11941 gaaccggcgc tgcgcgaagg tttaccgctg cgtcgtagcg actgggatgc atatctccag
    12001 tggggcgtgg aggccttccg tatcaacgcc gccgtggcga aagatgacac acaaatccac
    12061 actcacatgt gttattgcga gttcaacgac atcatggatt cgattgcggc actggacgca
    12121 gacgtcatca ccatcgaaac ctcgcgttct gacatggagt tgctggagtc gttcgaagag
    12181 tttgattatc cgaatgaaat cggtccaggc gtctatgaca ttcactcgcc aaacgtaccg
    12241 agcgtggaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc ggcagagcgc
    12301 ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac ccgcgcggca
    12361 ctggcgaaca tggtgcaggc ggcgcagaac ttgcgtcggg ggtaaaatcc aaaccgggtg
    12421 gtaataccac ccggtctttt ctcattacag cgacttcttc ccaccatact gcttaaacca
    12481 ttccagcata cgctgccagc catcttctgc agatgcggca tgatagctcg ggcgataatc
    12541 agcgttgaat gcatgcccgg cgtccgggta cacgataatc tctgctttcg cattagcagc
    12601 ccgcagcgcc tggcgcatgg tttcaacgct ctcctgcgga atactgttat ccagaccgcc
    12661 atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt gtttcggtga
    12721 attcagcgac ttatcgcccg tcagtttgcc gtaccacgcc actgcggctt ttagctgtgg
    12781 attatgcgcg gcatacagcc aagtgatacg tccaccccag cagaatccgg tgatcattaa
    12841 acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga gatcggccag
    12901 cacctgcgag tcaggcactt ttgctaccag accgctaagc aacgtgggga tatcggcaaa
    12961 atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat acccctccag
    13021 cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt cctgcactac
    13081 aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag caggcatgtt
    13141 atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtccg gggtctgaac
    13201 gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca gattgttgtg ttgttgccat
    13261 ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca gcatgcaaca
    13321 aatcacattg cctgaatcgg ctcatctttt atgcagtcct gcagaatgaa gagtgattta
    13381 tgtgatttgc atcacctttg gtgggtaaat ttatgcaacg catttgcgtc atggtgatga
    13441 gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt ctgactaaac
    13501 cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg cctcactaaa
    13561 aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg tgtggaaaag
    13621 atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt cactacctgg
    13681 cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg cggcccgtct
    13741 acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct gcgtatcggt
    13801 acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac cacggcgtct
    13861 gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc tgtcgctgat
    13921 ttcgaatgta cgactgcgct ggttgaagct gcgaaatcca ttggcgcgac aactcacgtt
    13981 ggcgtgacag cttcttctga taccttctac ccaggtcagg aacgttacga tacttactct
    14041 ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat gggcgtaatg
    14101 aactatgaaa tggaatctgc aaccctgctg accatgtgtg caagtcaggg cctgcgtgcc
    14161 ggtatggtag cgggtgttat cgttaaccgc acccagcaag agatcccgaa tgctgagacg
    14221 atgaaacaaa ctgaaagcca agcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg
    14281 taattctctt ctcctgtctg aaggccgacg cgttcggcct tttgtatttt tgcgtagcgc
    14341 ctcgcaggaa atgcctttcc aactggacgt ttgtacagca caattctatt ttgtgcgggt
    14401 aagttgttgc gtcaggaggc gttgtggatt tctcaatcat ggtttacgca gttattgcgt
    14461 tggtgggtgt ggcaattggc tggctgtttg ccagttatca acatgcgcag caaaaagccg
    14521 agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa caacaaatta
    14581 cccaaagcga gcactggcgt gcagagtgcg agttactcaa taacgaagtg cgcagcctgc
    14641 aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg atggaagccg
    14701 cacagcaaca tgctgacgat aaaattcgcc agatgattaa cagcgagcag cgcctcagtg
    14761 agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg gttgatgagc
    14821 aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg gacggtttcc
    14881 gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga acgccatacc ctgacccacg
    14941 aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca ggaagcgatc aacctgacgc
    15001 gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtagta ttgacgcggg
    15061 tgctggaggc ttccggtctg cgtgaagggt atgaatatga aacccaggtc agcatcgaaa
    15121 atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg gctgccgcag ggaaaagatg
    15181 tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctattttaac gccgaagacg
    15241 actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac catatccgtt
    15301 tgctgggacg caaagattat caacagctgc cggggctgcg aactctggat tacgtgctga
    15361 tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg gagctgatca
    15421 ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac tacgctgctg gtggcgctgc
    15481 gcactatcgc caacctgtgg cgttatgagc atcaaagccg caacgcccag caaatcgccg
    15541 atcgtgccag caagctgtac gacaagatgc gtttgttcat cgatgacatg tccgcgattg
    15601 gtcaaagtct cgacaaagcg caggataact atcggcaggc aatgaaaaaa ctctcttcag
    15661 ggcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggagta gaaattaaac
    15721 gcgagattaa tccggatttg gctgaacagg cggtgagcca ggatgaagag tatcgacttc
    15781 ggtcggttcc ggagcagccg aatgatgaag cttatcaacg cgatgatgaa tataatcagc
    15841 agtcgcgcta gcccattggg agtagttaag ccgggtagaa atctagggca tcgacgccca
    15901 atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt ggataagtca
    15961 caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc ggatatggtc
    16021 gcccacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt gatgtcattt
    16081 ggtattcatc gtttgtggaa gcgattcacg attgattgca gcggcgtacg ccgtgggcag
    16141 accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc ccgcctggtc
    16201 ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa aatgggccgc
    16261 gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc gaacgctgag
    16321 gcgctgccgt tcccggataa cacctttgat tgcatcacca tttcgtttgg tctgcgtaac
    16381 gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc cggcggccgc
    16441 ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc ctatgatgca
    16501 tactccttcc atgtgctgcc gcgtattggc tcactggtcg cgaacgacgc cgacagctac
    16561 cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa agccatgatg
    16621 caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt tgtggcgctg
    16681 catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt tagtgacggc
    16741 aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga aaacggcccg
    16801 ctcgcgtctg ttgggcaaag tgctgcgcgt ggaggtaaaa ggcttttcga cgtcattgat
    16861 tctggtgttc agcgaacgcc aggttgatgt actgggcgaa tgggcaggcg atgctgactg
    16921 caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc gatcgccagc agcttgccgc
    16981 cctgattcgc agtggtgagc tggaagtgca gggcgatatt caggtggtgc aaaacttcgt
    17041 tgcgctggca ggtctggcag agttcgaccc tgcggaactg ctggcccctt ataccggtga
    17101 tatcgccgct gaaggaatca gcaaagccat gcgcggaggc gcaaagttcc tgcatcacgg
    17161 cattaagcgc cagcaacgtt atgtggcgga agccattact gaagagtggc gtatggcacc
    17221 cggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc gtgctgttga
    17281 tgccctgatc aaacggctgg aaaaactgga ggctaaatga cgccaggtga agtacggcgc
    17341 ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat ccccaaaatg
    17401 cgtatcaccc tgccgctacg gctatggcga tactcattat tctggatgcc aaatcggcat
    17461 aaagacaaac ttttaggtga gcgactacga ctggccctgc aagaactggg gccggtatgg
    17521 atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt ttccgccgca tattgccgat
    17581 cagctggcgt tattgcagga caaagttgct ccgtttgatg gcaagctggc gaagcagcag
    17641 attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt tgaaatcaag
    17701 ccactggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc gaatggtaaa
    17761 gaggtggtga ttaaagtcat ccgcccggat attttgccgg tcattaaagc agacctgaaa
    17821 cttatctacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg ccgtctacgc
    17881 ccaaccgaag tggtgcgcga gtacgaaaag accttgattg atgaactgaa tttgctgcgg
    17941 gaatctgcca acgccattca gcttcggcga aattttgaag acagcccgat gctctacatc
    18001 ccggaggttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg tatttacggc
    18061 attccggtgt ctgatgttgc ggcgctggag aaaaacggca ccaacatgaa attgctggcg
    18121 gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt ccatgccgat
    18181 atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa atatatcggc
    18241 attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct ggcagaaaac
    18301 tttattgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt cgattctggc
    18361 tgggtgccac cagataccaa cgttgaagag ttcgaatttg ctattcgtac ggtctgtgaa
    18421 cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt aaatctgttt
    18481 aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact ccagaaaacc
    18541 ctgctctacg tcgaaggggt aggacgccag ctttatccgc aactcgattt atggaaaacg
    18601 gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc gctggtgaga
    18661 gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc tgaattggtt
    18721 tacgacagtt tgcgccaggg caagtattta cagcacagtg ttgataagat tgcccgcgag
    18781 cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat tggcgctacg
    18841 ttagtattaa gtggcacatt cttgttggtc agccgacctg aatgggggct gatgcccggc
    18901 tggttaatgg caggtggtct gatcgcctgg tttgtcggtt ggcgcaaaac acgctgattt
    18961 tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc tttgtttaat catcatctac
    19021 cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat tgccgtcatc
    19081 gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct tggtgcgtcg
    19141 atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa aaccagtcag
    19201 gatgctgatt ttactgcgaa aactatcgcc gataagcagg cggatacgaa tcaggaacag
    19261 gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt gtttgatatc
    19321 ggttttagcg aactgctatt ggtgttcatc atcggcctcg tcgttctggg gccgcaacga
    19381 ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg cgttgcgttc actggcgaca
    19441 acggtgcaga acgaactgac ccaggagtta aaactccagg agtttcagga cagtctgaaa
    19501 aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc gatggatgaa
    19561 ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc tgaaaaggcg
    19621 agcgatgaag cgcacaccat ccataacccg gtggtgaaag ataatgaagc tgcgcatgag
    19681 ggtgtaacgc ctgccgctgc acaaacgcag gccagttcgc cggaacagaa gccagaaacc
    19741 acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc tgcaccttcc
    19801 ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg cttatcacgc
    19861 atctgattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc gtgatattcc
    19921 tgtgtctggt ctatttcgcc aatgacatct atcacctggt atccgcgcca ttgatcaagc
    19981 agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc tttacgccga
    20041 tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc tatcaggtgt
    20101 gggcatttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg ccgctgctgg
    20161 tttccagctc tctgctattt tatatcggca tggcgttcgc ctactttgtg gtctttccgc
    20221 tggcatttgg cttccttgcc aataccgcgc cggaaggggt gcaggtatcc accgacatcg
    20281 ccagctattt aagcttcgtt atggcgctgt ttatggcgtt tggtgtctcc tttgaagtgc
    20341 cggtggcaat tgtgctgctg tgctggatgg ggattacctc gccagaagac ttacgcaaaa
    20401 aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gttgctgacg ccgccggatg
    20461 tcttctcgca aacgctgttg gcgatcccga tgtactgtct gtttgaaatc ggtgtcttct
    20521 tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac gctgaagcag
    20581 aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc atatggagta
    20641 caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag accgtgatga
    20701 tgttgtagcg cgcgcttttg acgcgggagt taatgggcta ctcatcaccg gcactaacct
    20761 gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat tcgtcctgtt ggtcaacggc
    20821 gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag cgattattga
    20881 gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact ttaaccgcaa
    20941 cttttcgacg ccggaagagc aggaacgcgc ttttgttgcc cagctacgca ttgccgcaga
    21001 tttaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta tgacattact
    21061 ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg gcacacgcga
    21121 agagatgcag gcgtgcgtgg cgcatggaat ttatatcggc attaccggtt gggtttgcga
    21181 tgaacgacgc ggactggagc tgcgggaact tttgccgttg attccggcgg aaaaattact
    21241 gatcgaaact gatgcgccgt atctgctccc tcgcgatctc acgccaaagc catcatcccg
    21301 gcgcaacgag ccagcccatc tgccccatat tttgcaacgt attgcgcact ggcgtggaga
    21361 agatgccgca tggctggctg ccaccacgga tgctaatgtc aaaacactgt ttgggattgc
    21421 gttttagagt ttgcggaact cggtgttctt cacactgtgc ttaatctctt tattaataag
    21481 attaagcaat agcatggagc gagcctcacc atcgggttcg gtgaaaatgg cctgaaagcc
    21541 ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggttg ccggatcgac
    21601 aatgtctttc ggtttatata ccgatagctg atgaatcacc gccgatggga ctatcgctgg
    21661 cgacgcgcca aagcgcacga agtggctgac accgcgggtc gcgttgatag tcgtggtatg
    21721 aatcacttct ggatcaaatt ccacaaacag gtagttgggg aacaatggct cactgactgc
    21781 agtacgtttg ccacgcacga ttttttccag ggtgatcatc ggtgccaggc aattcacagc
    21841 ctgtctttcg aggtgttcct gggcacgttg aagttgcccg cgcttgcagt acagtaaata
    21901 ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc aaaagcttta
    21961 cgctaagtta attatattcc ccggtttgcg ttataccgtc agagttcacg ctaatttaac
    22021 aaatttacag catcgcaaag atgaacgccg tataatgggc gcagattaag aggctacaat
    22081 ggacgccatg aaatataacg atttacgcga cttcttgacg ttgcttgaac agcagggtga
    22141 gctaaaacgt atcacgctcc cggtggaccc gcatctggaa atcactgaaa ttgctgaccg
    22201 cacgctgcgt gctggtgggc ctgcgctgtt gtttgaaaac cctaaagggt actcaatgcc
    22261 ggtgctgtgc aacttgttcg gtacgccaaa gcgcgtagcg atgggtatgg gccaggaaga
    22321 tgtttcagca ctgcgtgaag tcggtaaatt attagcattt ctgaaagaac cagagccgcc
    22381 aaaaggtttt cgcgatctgt ttgataagct gccgcagttt aagcaggtgt taaacatgcc
    22441 gacaaagcga ctgcgcggtg caccctgcca acaaaaaatc gtctctggcg atgacgtcga
    22501 tctcaaccgt attcccatta tgacctgttg gccggaagat gccgcgccgc tgattacatg
    22561 ggggctaacc gttacacgtg gccctcataa agagcgacag aatctgggca tttatcgcca
    22621 gcaactgatt ggtaaaaaca agctgattat gcgttggctg tcgcatcgcg gcggcgcgct
    22681 ggattatcag gagtggtgtg cggcgcatcc aggtgaacgt ttcccgatct ctgtggcgtt
    22741 gggcgctgat ccggcaacca ttctcggtgc agtcacacca gtaccagata ctttgtcgga
    22801 atacgccttt gccggattgc tacgtggcac caaaaccgaa gtagtgaagt gtatttccaa
    22861 tgatctcgaa gtgcccgcca gtgcggagat tgtgctggaa gggtatatcg aacaaggcga
    22921 aatggcgcca gaaggaccgt atggtgacca cactggttac tataacgaag tcgatagttt
    22981 cccggtattt accgtgacgc atattaccca gcgtgaagat gcgatttacc attccaccta
    23041 taccgggcgt ccgccagatg aacccgcggt actgggagtg gcgttgaacg aagtatttgt
    23101 tcccattctg caaaagcagt tcccggaaat tgtcgatttt tacctgccgc cggaaggctg
    23161 ctcttatcgc ctggcggtag tgacaatcaa aaaacagtac gccggacacg cgaagcgcgt
    23221 catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg tgatcgtttg
    23281 cgatgatgac gttaacgcac gcgactggaa cgatgtgatt tgggcgatta ccacccgtat
    23341 ggacccagcg cgggatactg ttctggtaga aaatacgcct attgattatc tggattttgc
    23401 ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat gccacgaata aatggccggg
    23461 ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg tcgcacatat
    23521 tgacgccatc tgggatgaac tggctatttt taacaacggt aaaagcgcct gatgcgcgtt
    23581 tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa gctgtaaagt
    23641 gacctcggta gaagctatca cggataccgt atatcgtgtc cgcatcgtgc cagacgcggc
    23701 cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg acaaacgtcc
    23761 gttctcaatg gcctcgacgc cggatgaaaa agggtttatc gagctgcata ttggcgcttc
    23821 tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc ctcaaagatc atcaaatcgt
    23881 ggtcgatatt ccccacggag aagcgtggct gcgcgatgat gaagagcgtc cgatgatttt
    23941 gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt ttgctgacag cgttggcgcg
    24001 taacccaaac cgtgatatca ccatttactg gggcgggcgt gaagagcagc atctgtatga
    24061 tctctgcgag cttgaggcgc tttcgttgaa gcatcctggt ctgcaagtgg tgccggtggt
    24121 tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc gtgttaacgg cggtattgca
    24181 ggatcacggt acgctggcag agcatgatat ctatattgcc ggacgttttg agatggcgaa
    24241 aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgcc tgtttggcga
    24301 tgcgtttgca tttatctgag atataaaaaa acccgcccct gacaggcggg aagaacggca
    24361 actaaaactt tattcagtgg catttagatc tatgtcgcat ccggcattcg gcacacgatg
    24421 cctgatgcga cgctggcgcg tcttatcagg cctacatggg tgccggatcg gtagaccgga
    24481 taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc tcaaacaccg tcgcaatacc
    24541 ctgacccaga ccgatacaca tcgtcgccag accaaactga acgtctttgt gttccatcag
    24601 attcagcagc gtggtgctga tacgcgcacc ggaacaaccc agcggatgac ccagcgcgat
    24661 cgcgccaccg ttgaggttga tcttctcgtc aatctgctca attagtccca gatctttaat
    24721 acatggcagg atctgcgcgg caaaggcttc attcatttca aacacgccga tatcactgac
    24781 agaaagcccc gcttttttca gcgccagctt cgaggccgga accgggccgt aacccatgat
    24841 cgatgggtca caaccaacga ccgccatcga acgcacacga gcgcgcggct taagacctaa
    24901 ttcatgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg aaagtgcaga
    24961 agatgtgccc gccgttaccg taccgtttac tggatcaaac gccggacgca gcgtggcgag
    25021 ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa ttaaactgct tcaggacgcc
    25081 gtcggcatcg tgaccaccag ttgggatgat ttcattttta aatgcgcccg actgcgtggc
    25141 ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc tgcatttcac ggctgatacc
    25201 gtgcatacgc gccagcattt ctgccgttaa gcccatcatg cccgccgctt tggcgacatt
    25261 gcggctcagg ccagggtgaa aatcgacgcc gtgactcatc ggcacatggc ccatatgctc
    25321 cacgccgcca accagacatg cctgcgcatc gccggtcatg atcattcgtg ctgcgtcatg
    25381 cagtgcctgc atggatgaac cacacaagcg attgacagta accgccggga cagagtgcgg
    25441 cacttctgcc agcagcgccg cgttacgggc gatattaaaa ccctgctcca gcgtctgctg
    25501 cacacacccc cagtaaatat cgtcgagggc cgccgcttcc agcgccgggt tacgcgccag
    25561 caggctacgc attaaatgag cggagagatc ttctgcacgc acgttacgaa aagcaccgcc
    25621 cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg acaacctgtt ccattgtgac
    25681 tccttaagcc gttttcaggt cgccaaccgg acgggctggc tcaaccgggg gatagtacgg
    25741 ttcgttatga cgcgctttat tacgcagacc ttccggcact tcgtacagcg ggccgaggtg
    25801 ctgatattgc tgtgccatat cgaggtattt tgcgctgccg agggtgtcca gccagcggaa
    25861 cgcgccgccg tggaacggag ggaagcccag gccgtagacc agcgccatat ccgcttctgc
    25921 cggagtggcg ataatgcctt cttccagaca gcgcaccact tcgttgacca tcgggatcat
    25981 catgcgggcg ataatctctt cttcgctgaa atcgcgcttc ggctggctca cttctgccag
    26041 caggtcgtca acagcggcgt cttcttcttt cttcggctta cctttgctgt cttctttata
    26101 atgccagaaa ccgaggccgt tcttctgacc aaagcggttg gcatcaaaca gcgcgtcgat
    26161 ggcatcgcga taatctttct gcatccgctg tgggaagccc gctgccatga ctgcctgggc
    26221 gtgatgcgcg gtgtcaatgc ccacaacgtc cagcagatat gccggaccca taggccagcc
    26281 aaactgtttt tccatcactt tgtcgatctt gcggaaatcc gcgccgtcgc gcagcaactg
    26341 gctgaaaccg gcgaaatacg ggaacagcac gcggttgacg aagaagccgg ggcaatcgtt
    26401 aaccacaatc ggcgttttac ccatcttgct cgcccaggcg acgactttcg caatagtttc
    26461 gtcggagctt ttctcgccgc gaataatttc caccaacggc attcggtgga ccgggttaaa
    26521 gaagtgcatc ccgcagaagt tttccggacg ttccagcgcg ttggccagtt cgctgatagg
    26581 aatggttgaa gtgttagacg ccagcacggt atccgggcgt actttctgct cggtttctgc
    26641 cagcacggct tttttcactt tcgggttttc aacaatcgct tctaccacaa catccacgcg
    26701 ctcaaatccg gcgtagtcga gtgttgggtg gatcgtggag atcacgccag ccagtttcag
    26761 accatcgatc ttgccgcgct caagctgctt gttcagtagt ttcgcggctt cagtcatgcc
    26821 gagggctaac gacttgtcgt taatatcttt cataatcacc ggcacgcctt tccacgcaga
    26881 ctggtaagcg atgccgccac ccataatgcc tgcgcccagc acggcggcct gtttcggggt
    26941 ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca tattgatcgt taaggaaaat
    27001 gccgaccagt gcgcgagctt cgttggtatg cgccagcggg acaaagcttt tgttttctag
    27061 gtttaaggct tcttcacgac caaaacgagc cgcagcttca atggttttta ctgcggtgat
    27121 gggggccggg taatgttttc ctgccgtttg tgcgaccatc cctttagcga tggtgaagct
    27181 catggtggct tcaatcttgc tcagttttag cggttccagc ttcggctgac gtttcgcttt
    27241 ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc atcgcccctt caatcagttt
    27301 ttctgctttg actacgccat ccaccagacc gattttcagc gcctgatccg cgccgacatc
    27361 tttaccggcg gcaatgattt ccagcgcact gtcagcgccc agcatacgtg gcatacgtac
    27421 agaaccgcca aagcccggca tgatgcccag tttggtttcc ggcagaccga tgcgcagatc
    27481 cggcgtcgcc agacggtaat cggtcgccag cacgcattcg cagccaccgc ccagcgcata
    27541 gccattgacg gcagcaatgg tcggcaccgg cagatcttcc aggcgattaa acacgctatt
    27601 ggcaaagtgc agccactgac ttaactgttc ttcaggaacg aggaacaggg acaaaaattc
    27661 ggtgatatca gcaccgacga taaaggctgc tttgttcgaa cgcagcagca gcccttttag
    27721 atctgattgc tgttccagca cgccgatggc ctcgccgagg ctggcgacgg tcgcagtgtc
    27781 gagtttatta actgaacctg gggcatcaaa taccagttcg gcaatgccat cttccagcca
    27841 gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc tcctgaatcc gcaaggtgat
    27901 ctggtcgtac cagatgcatc gaagtgtgta ttttgtgtta aaaatatgca aataaaagat
    27961 taaagaaatg ccgatctgat cacgctcggc agaaatcacg ctctggatga acgatgtgct
    28021 aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa aaatggaatc actggcctcg
    28081 ctctataaaa atcatatagc tacattgcag gaacgaactc gcgatgcgct gacgcgcttc
    28141 aagctggatg cgttacttat tcactccggc gaactgttca acgtttttct cgacgatcat
    28201 ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg tgccggtaac tcaggtgcca
    28261 aactgctggc tgctggtgga tggcgtgaat aagccgaaac tgtggttcta tctgccggtt
    28321 gattactggc acaacgtcga accgctgccg acctccttct ggactgaaga tgtagaagtg
    28381 atcgcgctgc cgaaagccga tggcattggt agtctgttgc ctgctgcgcg cggcaatatc
    28441 ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg gtattgaggc cagcaatatc
    28501 aacccgaaag gggttatcga ctacctgcat tactaccgct ccttcaaaac cgagtacgag
    28561 ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaacg gtcatcgcgc ggcagaagaa
    28621 gcgttccgtt ctggcatgag cgagttcgat atcaatattg cctatctgac tgcgaccggt
    28681 catcgtgata ccgacgtacc ttacagcaac attgtggcac tcaacgaaca cgctgcggtg
    28741 ctgcattaca ccaaactgga tcatcaggcg tcggaagaga tgcgcagctt cctgctggat
    28801 gccggggccg aatataacgg ctatgccgct gacctgaccc gtacctggtc ggcaaaaagt
    28861 gacaacgatt acgcacagct ggtgaaagac gtaaatgatg aacaactggc gctgatcgcg
    28921 accatgaaag ctggcgttag ctatgtggat taccacatcc agttccatca gcgcatcgcc
    28981 aaattgctgc gtaaacatca aatcatcacc gatatgagtg aagaggcgat ggtcgaaaac
    29041 gatcttaccg ggccgtttat gccgcatggt atcggccatc cgctgggcct gcaggtgcat
    29101 gacgtcgccg gttttatgca ggatgatagc ggtacgcacc tcgcggcacc ggcaaaatat
    29161 ccgtacctgc gctgcacccg tattctccag ccgggcatgg tgttaaccat cgaaccgggt
    29221 atctacttca ttgaatcgct gctggcaccg tggcgtgaag ggcagttcag caagcacttc
    29281 aactggcaga aaattgaagc actgaaaccg ttcggcggca ttcgtatcga agacaacgtg
    29341 gtgatccacg aaaataacgt ggaaaacatg acccgggatc tgaaactggc gtgatggaaa
    29401 gctggttaat tcctgcggca ccggtcacgg tcgttgaaga gatcaaaaaa agccgcttca
    29461 ttacgctgtt ggcgcatacc gatggcgttg aggcggcgaa agcgtttgtt gaatcggtgc
    29521 gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg ggtggcgggt gcgccggatg
    29581 attctcaaca gctgggtttc tctgacgacg gggagccggc gggaacggca ggtaaaccga
    29641 tgctcgccca gctaatgggc agcggcgttg gggaaattac cgctgtggtg gtgcgctact
    29701 acggcggcat attgcttggc accggtgggt tagtgaaagc gtatggcggc ggcgtgaatc
    29761 aggcgctgcg ccagctaacg acccaacgca agacgccatt aaccgaatat actttgcaat
    29821 gtgaatatag tcagttaacc ggcattgaag cgttgctggg gcagtgtgac ggcaaaatta
    29881 tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc gcttccggcg gcgaaagtgg
    29941 ctgaattttc cgcgaagctg gcggatttta gccgtggttc attgcaattg ttagcgattg
    30001 aagaataatc cccacttcgt tttgcagaac taaggaagcg gcagagatgc attttcgcgc
    30061 cattacccga atcgttggac tactggtcat cttattttca gggaccatga ttatccctgg
    30121 gctggtagca ctcatctacc gggatggagc gggccgcgct tttacccaga ccttttttgt
    30181 cgccctcgcc attggctcta tgctgtggtg gccgaaccgc aaagagaagg gcgaactgaa
    30241 atcccgtgag gggtttctga tagtggtgct gttctggacc gtgctgggta gtgtcggtgc
    30301 gctccctttt atcttctcgg aaagcccgaa cctcacgatt accgatgcgt tttttgaatc
    30361 tttctctggc ctgaccacca ccggggccac tacgctggtg gggctggatt cgctccctca
    30421 tgccatcctc ttttatcgcc agatgctgca atggtttggc gggatgggga tcatcgtgtt
    30481 agcggttgcg atactgccta tcctcggcgt gggtgggatg cagctctatc gcgcagaaat
    30541 gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt gcggaaacgg cgaaaaccct
    30601 gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg gcgttgtggt ttgccggaat
    30661 ggatgccttt gatgccatcg gccatagctt tgcgactatc gctattggcg gcttctcgac
    30721 acatgatgcc agtatcggtt atttcgatag cccgactatt aacactatca ttgctatctt
    30781 cctgctgatc tccggctgta actacggtct gcacttttca ctgttaagtg ggcgtagtct
    30841 gaaggtttat tggcgcgatc cggaatttcg catgtttatc ggcgtacagt ttacgctggt
    30901 ggttatttgt accctcgtac tgtggtttca taatgtctac agttcggcgc tgatgacaat
    30961 taaccaggcg tttttccagg tggtgtcgat ggcgacaacc gccgggttta cgactgacag
    31021 cattgcccgc tggccgctct ttttgccggt actgctttta tgttcagctt ttatcggcgg
    31081 ttgtgccggg tcaacgggcg gtggcctgaa agtgatccgc atcctgctgc tgtttaagca
    31141 ggggaaccgt gaactgaaac gactggtgca tccgaacgcc gtctatagca ttaagctggg
    31201 gaatcgcgca ctgccggaac gtatcctcga agccgtttgg ggatttttct ccgcctatgc
    31261 attggtgttt attgtcagta tgctggcgat tatcgccacg ggcgtggatg acttttctgc
    31321 ctttgcctcg gttgttgcga cattgaataa cctagggcca gggcttggcg tggttgctga
    31381 taactttacc agtatgaacc cggtggctaa atggatcctg attgccaaca tgctgtttgg
    31441 tcgtctcgag gtctttacat tgctggtgct ctttaccccg actttctggc gtgaatgatg
    31501 gagtaatacg tgaaaacatt aattcttttc tcaacaaggg acggacaaac gcgcgagatt
    31561 gcctcctact tggcttcgga actgaaagaa ctggggatcc aggcggatgt cgccaatgtg
    31621 caccgcattg aagaaccaca gtgggaaaac tacgaccgtg tggtcattgg tgcttctatt
    31681 cgctatggtc actaccactc tgcgttccag gaatttgtca aaaagcatgc gacgcggctg
    31741 aattcgatgc cgagcgcgtt ttactccgta aacctggtgg cgcgtaaacc ggagaagcgc
    31801 actccacaga ccaacagcta cgcgcggaag tttctgatga actcgcaatg gcgtcccgat
    31861 cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt atcgctggta cgaccgtttt
    31921 atgatcaagc tgattatgaa gatgtcaggc ggtgaaacgg atacgcgcaa agaagttgtc
    31981 tataccgatt gggagcaggt ggcgaatttc gcccgagaaa tcgcccattt aaccgacaaa
    32041 ccgacgctga aataagcata aagaataaaa aatgcgcggt cagaaaatta ttttaaattt
    32101 ccccttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    32161 aacacgccgc cgggtcagcg ggattctcct gagaattccg
//
LOCUS       DAZGXS010000041        31922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGXS010000041 DAZGXS010000000
VERSION     DAZGXS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHKG5_002065"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHKG5_002065"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0868173.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            997..1617
                     /locus_tag="ACHKG5_002066"
     CDS             997..1617
                     /locus_tag="ACHKG5_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFP0868174.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1631..2305
                     /locus_tag="ACHKG5_002067"
     CDS             1631..2305
                     /locus_tag="ACHKG5_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0868175.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2539..3687
                     /locus_tag="ACHKG5_002068"
     CDS             2539..3687
                     /locus_tag="ACHKG5_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0868176.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3684..4598
                     /locus_tag="ACHKG5_002069"
     CDS             3684..4598
                     /locus_tag="ACHKG5_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0868177.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4658..5956)
                     /gene="eno"
                     /locus_tag="ACHKG5_002070"
     CDS             complement(4658..5956)
                     /gene="eno"
                     /locus_tag="ACHKG5_002070"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0868178.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6044..7681)
                     /gene="pyrG"
                     /locus_tag="ACHKG5_002071"
     CDS             complement(6044..7681)
                     /gene="pyrG"
                     /locus_tag="ACHKG5_002071"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0868179.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7909..8700)
                     /gene="mazG"
                     /locus_tag="ACHKG5_002072"
     CDS             complement(7909..8700)
                     /gene="mazG"
                     /locus_tag="ACHKG5_002072"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0868180.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8771..9106)
                     /gene="mazF"
                     /locus_tag="ACHKG5_002073"
     CDS             complement(8771..9106)
                     /gene="mazF"
                     /locus_tag="ACHKG5_002073"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP0868181.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9106..9354)
                     /gene="mazE"
                     /locus_tag="ACHKG5_002074"
     CDS             complement(9106..9354)
                     /gene="mazE"
                     /locus_tag="ACHKG5_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP0868182.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9432..11666)
                     /gene="relA"
                     /locus_tag="ACHKG5_002075"
     CDS             complement(9432..11666)
                     /gene="relA"
                     /locus_tag="ACHKG5_002075"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0868183.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11714..13015)
                     /gene="rlmD"
                     /locus_tag="ACHKG5_002076"
     CDS             complement(11714..13015)
                     /gene="rlmD"
                     /locus_tag="ACHKG5_002076"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP0868184.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13072..15828
                     /gene="barA"
                     /locus_tag="ACHKG5_002077"
     CDS             13072..15828
                     /gene="barA"
                     /locus_tag="ACHKG5_002077"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0868185.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16061..17401)
                     /gene="gudD"
                     /locus_tag="ACHKG5_002078"
     CDS             complement(16061..17401)
                     /gene="gudD"
                     /locus_tag="ACHKG5_002078"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0868186.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17422..18762)
                     /gene="gudX"
                     /locus_tag="ACHKG5_002079"
                     /pseudo
     CDS             complement(17422..18762)
                     /gene="gudX"
                     /locus_tag="ACHKG5_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(18764..20116)
                     /gene="gudP"
                     /locus_tag="ACHKG5_002080"
     CDS             complement(18764..20116)
                     /gene="gudP"
                     /locus_tag="ACHKG5_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0868187.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20551..21000)
                     /gene="yqcA"
                     /locus_tag="ACHKG5_002081"
     CDS             complement(20551..21000)
                     /gene="yqcA"
                     /locus_tag="ACHKG5_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0868188.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21018..21800)
                     /gene="truC"
                     /locus_tag="ACHKG5_002082"
     CDS             complement(21018..21800)
                     /gene="truC"
                     /locus_tag="ACHKG5_002082"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0868189.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(21800..22129)
                     /gene="yqcC"
                     /locus_tag="ACHKG5_002083"
     CDS             complement(21800..22129)
                     /gene="yqcC"
                     /locus_tag="ACHKG5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0868190.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22751..23296)
                     /gene="syd"
                     /locus_tag="ACHKG5_002084"
     CDS             complement(22751..23296)
                     /gene="syd"
                     /locus_tag="ACHKG5_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0868191.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23364..24212
                     /gene="queF"
                     /locus_tag="ACHKG5_002085"
     CDS             23364..24212
                     /gene="queF"
                     /locus_tag="ACHKG5_002085"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0868192.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24324..25688
                     /gene="ppnN"
                     /locus_tag="ACHKG5_002086"
     CDS             24324..25688
                     /gene="ppnN"
                     /locus_tag="ACHKG5_002086"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0868193.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26245..27534
                     /gene="sdaC"
                     /locus_tag="ACHKG5_002087"
     CDS             26245..27534
                     /gene="sdaC"
                     /locus_tag="ACHKG5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0868194.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27592..28959
                     /gene="sdaB"
                     /locus_tag="ACHKG5_002088"
     CDS             27592..28959
                     /gene="sdaB"
                     /locus_tag="ACHKG5_002088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0868195.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            29071..29826
                     /gene="xni"
                     /locus_tag="ACHKG5_002089"
     CDS             29071..29826
                     /gene="xni"
                     /locus_tag="ACHKG5_002089"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0868196.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(29881..31029)
                     /gene="fucO"
                     /locus_tag="ACHKG5_002090"
     CDS             complement(29881..31029)
                     /gene="fucO"
                     /locus_tag="ACHKG5_002090"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0868197.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(31057..31704)
                     /gene="fucA"
                     /locus_tag="ACHKG5_002091"
     CDS             complement(31057..31704)
                     /gene="fucA"
                     /locus_tag="ACHKG5_002091"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0868198.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7838 a         8361 c         7667 g         8056 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taacgccaca gaatttgtag agaatgataa
       61 tgattagtcc gggataggca agatggatga gttttcagag acgacaaaga atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttaaatatt tatgggtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgtcgccaa aagttcatcc agtgcttcaa tatcacgtac gcgtcccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tacagcgtac ttcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgg
      601 cgaccaatga cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcaggtt tatggcaaaa agtggattag atcataaaga
      961 ataaaatgaa gcagagtatt atgttttaag gaattaatta aaagggattt aaacacgctc
     1021 agaatattca tcgtaattat atttattttc aatttatcag gctgtggata taacgatatt
     1081 caaacctatg atgagcaggt taacgcctcc tggtctgagg tattgaatca atatcaacgt
     1141 cgcgccgatc ttattcctaa tcttgtcgcc agcattaaag gttattccag ccatgaacga
     1201 gatgtactgg aagccgtaac attggctcgt agccaggcta atcgcgccag tagcgatctt
     1261 cagcaaactc cgggagatga acaaaaatta caagcctggc aacaggctca agcgcaggtg
     1321 actcgcactc tcggtcagtt aacgataatt agtgagcgtt accctgagtt aaaatctcag
     1381 gaactctatc aaaatttaat ggtacagctt gaaggaagtg aaaatcggat tgccgttgcg
     1441 cgaggaagat atattaaagc cattgaacaa tataatgtca ctatccgcaa attccccgct
     1501 gtattaaccg caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc
     1561 gccgcagtaa gtaaagcccc aacaatagat tttagccaga atgctaatgc tcattaagcg
     1621 gggaagttct atgcgaaatt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc
     1681 cacacaaatt ccagtacctg aatcacccaa gtatgtgaat gacttaaccg gcacattaac
     1741 aaacagcgaa gtaaacaccc tgaccaacca gattaaagcc ctgacgcaaa aaaactacgc
     1801 acagttggtc gtgttagtgg ttgaaacaac aggcgatgaa accatcgagc agtacgcgac
     1861 gcgggtattc gatagctgga aaccgggtga taaagaccgc gatgatggcg ttctactgtt
     1921 agtcgcctgg caggatcata ccgtgcgtat tgaaatcggt tacggactgg aaggtattat
     1981 tacggatgcg cagtccggga aaattattcg caacagtatt attcctgcat ttaaaaaagg
     2041 cgacctggct ggtggtttgc aaaagggaat cagcgatatt gaaagtcgtc tgacggggaa
     2101 taatttagca acgataacac cgaccgatca ccctttgccc tttagcggtt ggtgggcatt
     2161 gttagtctgg gcgatagtac tgacgtttat ttctgccaga ggatatatca aaacgctggg
     2221 agtaatttgc ttcgcagcta ttgtactggc ctttgtttta cctatagcag gcttcagcgg
     2281 aagctgggga gtactcgcaa catgactctg cttcgccaca ccgttcctgg ctgtcgccat
     2341 cgcttttacc cccttcggca aaaaagttcg ggatttgatg cgtgatgcca accagccatc
     2401 acgccataca agaagcaatt cttcctccca ctattcatca tcgtcatcct cttcattcga
     2461 caatgacaat tttagcggtg gtggcggttc atctggtggc ggtggcgctt ccgggcgctg
     2521 gtaacaaaag gaattgatat gcgtattttt ctcttacttc tgatgctatg cggttttcag
     2581 gtattcgctg aacgattgcc ttcagtgact gacttaaccg ggacattaac gacagaagaa
     2641 caaactgctt taactcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc
     2701 gtgttggtta cgccgaccac agggggcaaa aacattaaac ttgcagcctc gcagagatac
     2761 ggggtatatc actggaaacc cgttggagag aagcgatatg gcgaaggtat cttaattctc
     2821 gtcgtatggc cggaagggct ggcgtcaatg aagattggac atggactgga agagatgttg
     2881 ccgccggaac aagctgcgca aattgttcgt taccatatgc agccagaatt cgaaaaaaac
     2941 aatctctttg ctggattaac gggcggaatt gaaagcatcg cacagtttac ccatatcaaa
     3001 gcgaacctta gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaacgttcg
     3061 ctgccatgtc ttgcctggac actgctgatg atcgttgcca tcgttgtcct ctggctattt
     3121 gccagccgcc cacggcgtgg catatggatg atatcgatga ttacccctat ggtctggttt
     3181 ttctcctttc aggacgatgt catcattcgg cgggtatcgg tcgtttgttt tagtttattg
     3241 ttagcaacaa cctgcaagca gcgtctcgcg gtagttttta atatgtgccg cgtatttcct
     3301 atgatgctga ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagaa gactccgcag
     3361 gttcgtgaat caggacgaaa ttcattgttt gtcgtgatgc tgataattat tgtgggatgg
     3421 tgcctggtgt tcgccagtaa taaagatatt gcattcatct caacaattat tgggttaatt
     3481 gatcatttta tcggcccaat aaccattgct ggcatcgttc tgctgatcat tgtggccaaa
     3541 tttactggca atcttaagct ggggtcagga gaaaaaagca acaggaaaac ggggaatcgt
     3601 aatgctcatt ctcgctcttc gtcgagaaat tctttccgcg gtggtggcgg ttcttcagga
     3661 ggcggcggtt catccgtccg ttggtgatcg taatgcgctt aatctttatt ctattcactt
     3721 tgtggtgcct tcctgggctt gcccagcaaa ttgctgtgcc ggaactgcgc caacaggtga
     3781 ccgatattac tggtacgtta agcacttctg aacaacaatc actgacacag caactgcagg
     3841 atattacaca taaaacacgg gcgcaggtcg ctgtattagt agtgccgtcc accggagacg
     3901 attccattga acaatatgct acaagggttt ttgatagctg gaaactggga gataagcaga
     3961 gaaacgatgg tattttactt ctggttgcct gggaggatca tgctgttcgt attgaggttg
     4021 gctacgggct ggaaggggtg gttaccgacc tgcaagcggc gaagattatc agggatatat
     4081 tgatccctgc cttcaaaagt gatgacctga tgggtggatt aacactggca agcgaaaata
     4141 tcggcgcgct tctgttgaat ggtgaattac cggaagacag gggggattat tacagtatca
     4201 atccccctat tccattatcg cttgctgtaa ttatattgct ggcggtactt tcatatttta
     4261 ttgtttttac cgatccgtcg aatttaccct ggatcactct cactggcgcc atttacggga
     4321 tggtattcct ctatgttgcc gagccaggac catggaccaa tttaatcgtt gcctgcggta
     4381 tgttaacgcc ttttgcgatc gtaccactgg ttattttttg gcttatcgtc aataaaaagc
     4441 tacgcgccaa atacaaaaaa ctcagtaaag atagagcttc aagaaaaggt tcttcttcat
     4501 cctcttccgg tggcggctca agtggcggag gctcaggcgg cggatttagc ggcgggggcg
     4561 gttcttctgg cggtggcggc gcgtccggtc gctggtaact caaccataaa aaacgccagc
     4621 ccgaaggctg gcatttttaa atcagataaa gtcagactta tgcctggcct ttgatctctt
     4681 tacgaccgtt gtacggtgct ttttcgccca gagcttcttc gatacgaatc agctggttgt
     4741 atttagcaac gcggtcagaa cggctcatag aaccagtttt gatctggcct gcagcagtac
     4801 caacagccag gtcagcgatg gtagcgtctt cagtttcgcc agaacggtga gagataactg
     4861 cagtgtagcc agcgtctttc gccatcttga ttgcagccag agtttcggtc agagaaccga
     4921 tctggttgaa tttgatcagg atggagttag cgataccttt ttcgatacct tctttcagga
     4981 tcttggtgtt ggttacgaac aggtcgtcac caaccagctg gattttgtcg cccagaactt
     5041 tggtctggta tgcgaaacca tcccagtcag attcgtccag accgtcttcg atggaaacga
     5101 tcgggtactg tttggtcagt tcttccagga agtgagtgaa ttcttcagag gtgaatgctt
     5161 tgttgccttc gccagccaga acgtatttac cgtctttgta gaattcagaa gctgcgcagt
     5221 ccatcgccag agtgatgtct ttgcccagtt cataaccagc agctttaaca gcttcagcga
     5281 taacagccag agcttcagcg ttggaaccca ggttcggcgc atagccacct tcgtcaccaa
     5341 cagcagtgtt catgcctttc gctttcagaa cttttgccag gtgatggaaa acttcagaac
     5401 ccatgcggat ggcttctttc acagttttcg cgccaaccgg ctgaatcatg aattcctgga
     5461 tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat gttcatcatc ggaaccggca
     5521 tggagtattt gcccggagtg ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt
     5581 tagcagctgc agcagctttg gcgttagcca gagatacagc caggattgcg ttcgcgccga
     5641 atttggattt gttttcggtg ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat
     5701 ctttagcatc tttgccaatc agcgcctgag cgatcgggcc gtttaccgca gcaacagctt
     5761 tggttacgcc tttacccagg aaacgggatt tgtcgccatc gcgcagttcc agagcttcac
     5821 gggaaccagt agaagcacct gacggagcag ctgccatacc gacgaaacca ccctccagat
     5881 gtacttcggc ttcaacagtc gggttaccac gggagtcgat gatttcacga ccgatgattt
     5941 ttacgatttt ggacattagg ttttcctcaa gtcactagtt aaactgaaac tccagacaaa
     6001 caacgcgtac ccagggtacg cgttgccgtt ctaacttttt tacttacttc gcctgacgtt
     6061 tctggaactc gctggcggct ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag
     6121 tagaagtaaa ctccggatgg aactggcaag ccacgaacca cgggtgattc ggaacttcga
     6181 tgatctcgac caactgatca tccccggaac ggcccgcaac gcgcagacct gcatcttcaa
     6241 tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg atgacgctca acaattgtcg
     6301 gcgcattgta cagctggcga accaggctat cgtcaaccaa ctggcactgc tgtgcgccga
     6361 gacgcatggt accgccgaga tcgctcttct cgctacgaac ttcaacgttg ccgttttcat
     6421 cgcgccactc ggtaatcagc gccacaaccg ggtacttaca gtctggcaca aattccgtag
     6481 agttggcgtt ctccatgttg gcaacatggc gagcgtaatc aattaacgcc acctgcatac
     6541 ccaggcaaat gcccagataa ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca
     6601 tgccttctac gccacgatag ccgaaaccgc caggtacgag gattgcatcc agacctttaa
     6661 ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat cagtttgatg ttgacgctga
     6721 cacgattctt cagcccaccg tgtttcagtg cttcaatcac ggatttgtaa gcgtccggca
     6781 gttcaatgta cttgccgacc ataccaatgg tgacttcact taccggattc gcttcttcga
     6841 agataacctg ttcccattcg gacagatttg cttccgggca gtttaagctg aatcgtttac
     6901 aaatataatc gtccagcccc tgagatttca acaggcccgg aattttatag atggaatcga
     6961 cgtctttcag agaaataacc gctttttccg gaacattaca gaacaatgca atcttcgcac
     7021 gttcgttcgc cggaacggcg cgatctgaac gacaaatcag gatgtcaggc tggataccga
     7081 tggagagcag ctcttttaca gagtgctgag tcggtttggt tttgacttca ccagacgctg
     7141 ccatgtacgg caccagcgtc aggtgcataa acagagtgtg ctcacggcca atttcaacag
     7201 ccatctggcg aatcgcttcg aggaacggca gggattcgat atcacctact gtaccgccga
     7261 tttcgaccag tactacgtca tgaccttcgc caccttccag cacgcgctct ttgattgcgt
     7321 tagtgatgtg cggaataacc tgtacggttg cgccgaggta gtcaccgcgg cgttctttac
     7381 gcagaacgtc agagtagata cgacccgtgg tgaagttgtt gcggcggctc attttggtac
     7441 ggatgaaacg ctcgtagtgc cccaggtcca ggtcggtttc agcgccgtct tcagtaacga
     7501 acacttcccc gtgttggatt gggctcatag tacctggatc gacgttgatg tacggatcca
     7561 gtttcatgat ggtcacattg aggccacggg cttcaagaat ggctgcgagg gaggctgcgg
     7621 caatgccttt acccagagag gatacgaccc cgccggtcac aaaaatatag ttcgttgtca
     7681 tgctgaacct gagaagttag gttgaaaaga cgatggaata accaggacgg gaaagcagta
     7741 tacccgaaca tgacctgtgc cacaaacttt cattatccct cctcttcgcc agcgcactat
     7801 tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg tttttgacgc aaatcaagcg
     7861 cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg taattccctt agagatcaat
     7921 ttcctgccgt tttacctgtt gccagacttc ttccattgtt tcgaggtcaa cacctgtcat
     7981 ttccagtcca cgcgcggcaa caatacgctc cacttcgcga aaacgacgct cgaatttttc
     8041 gttcgctttt tgcaatgcga tttccgcttt tgtccctaaa tgacgagcca gattgaccgt
     8101 ggcaaacagc aggtccccca tttcctcctc cagtttagcc tggtcgacaa cagcctgccg
     8161 cgcttcgtac atcacctcgt cgatctcttc gtagacttta tcgactaccg gaccaagcgt
     8221 cgtccaatcg aagccaacgt tggcgcaacg tttctggatt ttttgcgcac gcattaaagc
     8281 cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc gctttctgcg cgcgctcttc
     8341 ggttttgatt tgctcccaac gggcaagcac ttcactactg ttttcggcag aactgtcagc
     8401 aaaaacatgc ggatggcgac gctctaattt atcgctaatc gcagcgcaaa tatcattaaa
     8461 gtcaaagcgc ccttcttcct gagccatttg ggcgtaaaac accacctgga acagcagatc
     8521 gccgagttca ccgcgcagat cgtcaaaatc ttcacgggcg atggcgtcca gcacttcgta
     8581 ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc tgctctttat cccacgggca
     8641 gccgttttcc gggtcgcgca ggcgctgcat aatagtgagc aaacggtcga tttgattcat
     8701 tagaatgtcc tgaaaattgc gggtccgtca ggtggaaacc tgtgaccaga atagaagtga
     8761 gttagtaaca ctacccaatc agtacgttaa ttttggcttt aatgagttgt aattcctctg
     8821 gggcaaccgt tcctttcttc gttgctcctc ttgcccgcca ggcgatactt tttacctgat
     8881 cagctaacgc tacgccatca cgttcctgac cggataaaac aacttcgaac ggatatcctt
     8941 ttgattgcgt tgtacaagga acacacagac acatacctgt tttgttgttg tacatgaacg
     9001 gactcaggac aacagccgga cgatgtccgg cttgctcgct accttttgtc gggtcaaaat
     9061 caacccaaat cagatcgccc atatcgggta cgtatcggct taccattacc agacttcctt
     9121 atctttcggc tctccccagt cgatattctc gtggaggttt tccggcgtga tgtcgttgac
     9181 cagttcagca agcgtaaata cgggctcttt acgcactggc tcaataatta atttgccatc
     9241 caccaggtca atcttcactt catcatcaat attcagattg agcgcctgca ttaacgtagc
     9301 cgggatccgc accgccggtg aatttcccca acgctttacg ctactgtgga tcataaccct
     9361 ttcctcaaac cactatcata tgtagataca gtatatatca atctacattg tagatacgag
     9421 caaatttcgg cctaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     9481 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     9541 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     9601 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     9661 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     9721 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     9781 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     9841 atgtggtgca tcaggttacc aacaccttca accactacac gaccgttatc tttactgcgg
     9901 ttttgcggcg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     9961 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
    10021 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
    10081 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
    10141 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
    10201 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
    10261 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
    10321 acaatgcgcc cgccaatttt tgccccgata cagcggtgtc cgacatcact gtggatgtgg
    10381 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttaggcgta
    10441 aagacgtaca cccggtcgtc aaagacctgg ctgcgtactt cgtcgagcat ttcgccggaa
    10501 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
    10561 cccgaacgtg cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc
    10621 aactctgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
    10681 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
    10741 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
    10801 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
    10861 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
    10921 acttcagctt taacgccttc agctttcatc tcagcgcgca gatgaccgac aaactcttcg
    10981 atatagtgtt cgcggtcgag acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg
    11041 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
    11101 agacggtttg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacgcgttca
    11161 tcttccggcg catcttttac ttcacgcaga tgagcaatac gctccgccag tttgatgacc
    11221 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
    11281 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
    11341 ccgtgaataa ggttaacgac cgacttaccg acgctctcac gcagcacatc ttcgctgact
    11401 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
    11461 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
    11521 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
    11581 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
    11641 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
    11701 cggcaaatcg caactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
    11761 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
    11821 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
    11881 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
    11941 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
    12001 aaaacgtcac attctgtaag cagttaagac gcgcattctg ctggcctttt tccaccagcg
    12061 ccggaacacc ttctacaccc accacactgg cagcttgtgt cgccaatggt agtgtaaagt
    12121 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttccacctcc agccattcca
    12181 acgcacgcgc taccattttt tggttcacac ccgcattaac ctgaataaaa tcgcgcgggc
    12241 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
    12301 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
    12361 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
    12421 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccaggc
    12481 atgccctgac tttgggcagc aatgcttcaa gttggggcac taaaatggga cattgtttaa
    12541 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
    12601 gtaagtagtt cagacttaaa cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca
    12661 cttcagagac ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
    12721 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
    12781 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
    12841 ttttatcctc agtaacagta acttccgcgt tttcctgcgg caataatccg gggataaata
    12901 gcgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
    12961 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
    13021 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
    13081 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
    13141 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
    13201 gccggtgcca gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
    13261 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
    13321 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
    13381 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
    13441 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
    13501 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
    13561 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg
    13621 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
    13681 ggtccgattc gcaacatggt gaatactgtc gaccgcatcc gtcgcgggca actcgacagc
    13741 cgagtggaag gatttatgct cggagagctg gatatgctga aaaacggtat caactcgatg
    13801 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
    13861 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
    13921 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
    13981 ctgcgtacac cactgaatgg tgttattggt tttacccgcc tgacgctgaa aacagaatta
    14041 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
    14101 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
    14161 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
    14221 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
    14281 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
    14341 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
    14401 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    14461 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    14521 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    14581 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    14641 attattattg aagggccatc cacccagtgc ctcgccggta aacgcctggc ctatgtcgaa
    14701 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    14761 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    14821 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    14881 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa
    14941 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    15001 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    15061 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    15121 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg
    15181 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgcctgac
    15241 atggatggca ttcgggcctg cgagctcatc caccagctcc cccatcagca acaaacgccg
    15301 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    15361 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    15421 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    15481 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    15541 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    15601 gttgaggaac agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    15661 catggcagtt gcggctatag cggtgtgccg cgtatgaaga atctctgcca acttatcgaa
    15721 caacagctac gtagcggtac taaagaagaa gatttggaac cggagctgct ggaactgttg
    15781 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    15841 cgagtagcaa tcggcttttt atgacgctgt tttcagattg gtgacaaagt gcctgtgttt
    15901 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaataaatt
    15961 aaaaagcatg taggcctgat aagcgtagcg catcaggcaa ttttgtgttt ggcatcagtc
    16021 tcaaaccggc tccagataga gccggttttg gttttttgtc ttaacgcacc atgcacgggc
    16081 gcttgttatc gaacgtccag ccaggaatca gatactgcat tcccatcgca tcgtcacgcg
    16141 cgccaagccc gtgtttctga tacagctcat gggctttcat cacttgatcc atatcgattt
    16201 ctacacccag ccccggtttt tctggcacct gtaccagccc gcctttgatc tcaaacggtt
    16261 ctttggtcag gcgctgatta ccttcctgcc agatccagtg cgtatcaata gcagtaattt
    16321 tacccggtgc agcggcggca acatgggtaa acatcgccag ggaaatatcg aagtggttgt
    16381 tagagtgtga accccaggtc aggccaaatt catggcacat ttgcgccaca cgtaccgaac
    16441 cttgcattgt ccagaaatgc ggatccgcca gcgggatatc aacggattgt aacgagagcg
    16501 tatggcccat ctgccgccag tcggtggcga tcatattggt tgcagtcggc aggccagtcg
    16561 cgcgacggaa ctctgccatc acttcacgcc cggagaaacc ttgctccgca ccacacggat
    16621 cttctgcata agccagcgaa cctttcaggt atttgccgat tttaatcgct tcgttcagcg
    16681 accaggcacc gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca
    16741 caatagactc ggcctcttct tccccggcca gtacaccgcc tttcagtttg aaatcgttga
    16801 agccatattt ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt
    16861 cttcatgacg caggcgatac cagtcgcatg agtcatccgg ctggctttga tacggcagag
    16921 gtgtggcttt gcgattaccg acgaagaaca gataaccgag catttcgact tcgctacgct
    16981 gttgaccatc gcccagcagg gatgccacgt taacccccag atgctgcccc agcagatcca
    17041 gcattgccgc ttctatcccg gtaactacat gaatagtggt acgcaaatca aatgtctgca
    17101 aaccgcgccc gccggcatca cgatcggcaa aagtattacg caccagcgtc agaacgtttt
    17161 tgtattcacc cagcgtttta cccactacca gcggaattgc atcttccagc gttttacgga
    17221 ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc agaattatct ttgataatca
    17281 caatattacg cgtgaagaac ggtgcgtgtg caccactcag attcatcagc atactgtcat
    17341 ggcccgccac cgggatgacc tgcatttcag taacaacagg cgtcgtaaat tgagaactca
    17401 tatttatgtc ctttttcaga atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt
    17461 ccagccgggg atcaggtact gcatcgggcc tgcgtcgtta cgcgcaccgc caggcagacg
    17521 tttataggcc tcatgtgcct tttgtacctg ttcccagtcc agttccacgc ccagaccggg
    17581 cgcatcagga acggcaattt ttccgttttt aatctccagc ggattttggg tcaggcgaca
    17641 atcgccctcc tgccaaatcc agtgggtatc gatagcggta ggattacccg gtgccgccgc
    17701 gcccacatgg gtaaacatcg ccagagagat atcgaaatgg ttgttggaat ggcagcccca
    17761 ggtcagcccc cagtcgtcgc aaagctgcgc cactcggact gcaccggaaa gcgtccagaa
    17821 gtgcggatcg gcaagtggaa tatctaccgc attgagcatc accgcatgac ccatttcgcg
    17881 ccagttggtg gcgatcatat tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc
    17941 catcacttca cgtccggaga agccctgttc tgcgccgcat ggatcttcgg cataggtaag
    18001 aacatcattc agccctttgc acagagaaat ggcttcatca agcagccatg caccgttggg
    18061 atcaacggta atccgcgcat ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc
    18121 ttgctcgcca ggtaacacgc cgcccttaag tttgaaatct ttaaagccgt agcgatcctg
    18181 tgaggcttcc gccagacgca caacggcttc gctgttcatc gctttctgat ggcgcaactg
    18241 ataccactca tggttgcccg gcgtattttc cagataagga agatcggttt tggtccgatc
    18301 accgatataa aacagataac cgaggacggt aatagcatcg cgttgcttgc ctggccctaa
    18361 cagttcgcag accggaacat tcagcgcctt acctagcagg tcaagcaagg cggcttccag
    18421 cgccgccacg gcgttaacgc gcaattcaaa ggtccaggcc cctttaccga aggtatcaaa
    18481 atcggctgct tgattacctt tatgcacctg ctgaaccact ttattcaggc gcgcaacttc
    18541 ctagcccagc accatcggaa tagcatcgac caatgtctga taaatcacct ctccgcctgg
    18601 cgcttcacca atgccggtat gcccggcgtt atcggtgagt accacaatat tgcgagtgaa
    18661 atatgcgtta tgcgccccac caatattaag caacatgctg tcgtacccgg cgaccggaat
    18721 gactttcata tcagtaataa caggactgga ttgtgtcgtc atcttattgc cccacgacag
    18781 gtttcagctc gatacgcttg atatcgccca ccagcaccag gtagctcagt accgcgatta
    18841 aggcatgaac accaacataa atcagcgccc cattaaacga gccagtcgtg ccaacgatat
    18901 aaccaattgc gattggcgtg acgatgccag aaatgttacc gaacatgttg aacaggccac
    18961 cggaaagacc gctgatctct tttggcgcgg tatctgccat tactgcccaa cccagcgccc
    19021 caatgccctt accgaagaag gccagcgcca taaagccgat gatcatccac tcaacgttga
    19081 cgtagttgca gaacaccatc accatcgaca gcaacatgcc cattacgatc ggtgttttac
    19141 gcgcaatgtt cagcgatccc gtgcggcgca tcagccagtc ggaaataatc ccacccagca
    19201 caccaccgat aaaaccacaa accgccggaa cggaagccac aaagcccgct ttcagaatcg
    19261 acatcccacg cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag taagtcaggg
    19321 cgttgataca gtactgaccg atataaacgc cgatcatcat ccgtgacccg agcagttgtt
    19381 tgatctgccc ccacttcacg ctgaacggca ctttaacttt ggtgttttgc tgatccatat
    19441 tgatcagcgc gccacccgcg gcgatgtact ccagctcttt ctgatttacc cccggatgtt
    19501 gatttggctc gtggatgact ttcaaccaga taaagctgat ggcaatgccc agaccgccca
    19561 taaagaagaa gacgtgtgac cagcccactt catgcgtcag ccagcccata atcggcgcga
    19621 agatcaccgt tgcgaagtat tgagcggagt taaaaatcga caccgccgtt cccctttcct
    19681 gcgccggaaa ccaggccgca acaatgcgac tgttgccggg gaaagatggc gcttcagcaa
    19741 gcccgaccag gaagcgcaga gtaaacaggg caacgataat gccgaatcca ctaaagatat
    19801 cgacgaagcc ttgcagcaag gtaaacatcg accagataaa gatcgaccag aagtagacgc
    19861 gttttgaacc aaaacgatcc agcaaccaac caccagggat ctgcccgata acataagccc
    19921 atgagaaggc agagaacaca tagcccattc ccacgggatc aaggccgata tctttggcca
    19981 tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa
    20041 acaacatcac cactatccag taacgagcat tcgtgcgttt ttccacactg ctcgcagcct
    20101 gacttaaaga actcattgtt gcactcctga aaattcgcgt tagccacgct cactctggac
    20161 tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt tttgccgttt ttataggtcg
    20221 ttcgccgaat acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc
    20281 ggctcaccgt gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc
    20341 cctggacggt ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa
    20401 acgaagaaat aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa
    20461 atccgcgcta tgaagcagtt tttactggca tttgcctgaa aagattcgat tcagcaccgc
    20521 taaaacgaca tttaccgctc gctgaacata tcaggacaac agcgtgcccc actgttcgac
    20581 ccacggattt gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc
    20641 gccaacccgc tgagcgctct gttcctgcag caaggcatcg aactgtttgc cgccattgca
    20701 gaaattcaca taactactgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa
    20761 gcccagacta tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg
    20821 cccggtcgtg gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa
    20881 ttcaggatct tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc
    20941 ttccgccact aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat
    21001 accgttttct ccctggatta ggaacttctc tctccatcct gacccgatgg cgcactgaac
    21061 tcaacccttt cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc
    21121 caggtgtcgt ccaggcccgc gtgaatagtc agcggctcac cagtaaaagg atgcgtcaat
    21181 gacaactgac tggcatgcag cattaaccgc tgaagaccaa aatgctcagc accgctgcga
    21241 ttctggcgta aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga
    21301 tgtcggcgaa gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc
    21361 gtggtcgggt aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc
    21421 gtcactgctg gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt
    21481 tcttccacca gtggataatc cagcaccgct tcttccatca accagccgcg cacaatcgca
    21541 tggtaacgtt tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg
    21601 ctggataatc ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa
    21661 acatgctggc ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc
    21721 agccagctac ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc
    21781 tgatagagta tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca
    21841 actcagccag aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg
    21901 gtgcaacggc aaaagcgccc ggtaacggct ggttgttatt taacaaatcg tgcattcgcg
    21961 ggatcagcac ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg
    22021 tactattaaa ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta
    22081 acgcttcaag cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc
    22141 aataagaaaa actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag
    22201 tgctcccggt tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac
    22261 aacttttcct ctggccatgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac
    22321 caccccgatg tgatacttca tcctgaagtg tcccaggcct tcctgaccca ccgaatcatc
    22381 ctgaccggtt ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct
    22441 tcgcttcatc cagaagcctt tccctgaaac accatcctgg tgtgtccttc agaagtgtca
    22501 tcatcctgat gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt
    22561 cgcgaactct tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta
    22621 ggaattaaca agcaacatgt tgtttttaaa tgaaaataac gattcttatc tttttaagta
    22681 ttcgcttatt tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg
    22741 ggtatttgga ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttggggcta
    22801 aatgcgtccg tttacgtgtt cccagcgtct ctttacacac ttcaccggag agattacaca
    22861 ccgagatgac ctctaactct tcttccagcg tcgcgataaa tagcgtgggt ggcagtttta
    22921 gccttttctg agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt
    22981 cactccaggt ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg
    23041 caaactgagt cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt
    23101 caaccgcgtt tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt
    23161 cagtagttga aataatgcac ggtgaaggaa cgccgtacag ttcctcactt aacggccagc
    23221 ttttatgctc ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg
    23281 cggtcaaatc gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt
    23341 tatctggttt atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc
    23401 tgactcttgg aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg
    23461 ttccacgcag cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcaag
    23521 gtacggatat ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg
    23581 tcgctgtcgg tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt
    23641 ttaagctcta tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc
    23701 agacgctgga gcgcgactta agcacttgcg ctcagggtga ggttagcgtg gcgttatatc
    23761 gtcttgatga actggaaggc cagccgatag gtcatttcaa tggcacttgc attgatgacc
    23821 aggatatcac tatcgataac tatgaattca ctactgacta tctggagaat gccaccagtg
    23881 gtgaaaaagt agtggaagag acgctcgtca gccacctgct gaaatcaaac tgcctgatca
    23941 cccatcaacc agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag
    24001 aaaaactgct gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg
    24061 tggaacgcat ctttaatgac ctgttacgct tctgccagcc agagaaattg agcgtttacg
    24121 cacgttatac ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg
    24181 tcccatcgac cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt
    24241 cacgcagaag gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat
    24301 attcgtccca taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata
    24361 tgttgtcgca gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac
    24421 tgtttcgcaa ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag
    24481 aattgctgtc tcgttttgaa aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa
    24541 agctggaact gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt
    24601 tgcaggccaa cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata
    24661 ataccgttcg ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct
    24721 tttccatctt gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct
    24781 gctggggcgg tcactcaatt aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc
    24841 agctgggcct gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc
    24901 cgatgaaagg tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg
    24961 gtatgacaga gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga
    25021 tcatcatgcc ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca
    25081 ttatcttccc tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa
    25141 tgaacccggc caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg
    25201 ccgactactt ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc
    25261 gccattaccg cataatcatt gatgacgccg ctgaagtcgc ccgtcagatg aaaaaatcga
    25321 tgccgctggt gaaagaaaat cgccgtgata caggcgatgc ctacagcttt aactggtcaa
    25381 tgcgcattgc gccagatttg caaatgccat ttgagccgtc tcacgagaat atggctaatc
    25441 tgaagcttta cccggatcaa cctgttgaag tgctggctgc cgatctgcgc cgtgcgttct
    25501 ccggtattgt ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc
    25561 cttacaaaat caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt
    25621 ttgttgccca gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct
    25681 gcacgtaatc tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcact
    25741 tactcatcaa ctcatttcat ttgttatatg aatgtttctt accaccctca cgcgacaaat
    25801 atcatcacag ttaatatgtc atacaattta tattgcaacg caaacgtttc cctattttca
    25861 taaaccgtat tttatcgcta tgaaaaagaa attatcgcca tgattaacta aaagtattga
    25921 ttttttcagt tcaacctaca tacattgcgc gccccggaag aagtcagatg tcgtttaatg
    25981 ggcaaatatt gcccttaaat tctcttttac ttttgattta cagagtaaag cgttgggata
    26041 atctatcttc caagtagatt attgtatttg ggatcaagat cactgataga tacataactt
    26101 gtgtgtatct ttccgccctc aaattattac ggcggtaaat gattaagcca tcgccgatag
    26161 acagatttca tttttacggt caggcacctt cccgggctga actggctaaa agctgaatta
    26221 tttgcattcc tccaggagaa atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa
    26281 gactctcgta gtgcctggcg caagacagac accatgtgga tgctgggcct ttacggcacg
    26341 gcaatcggcg cgggcgtgct gttcctgcca atcaacgccg gtgttggcgg tatgatcccg
    26401 ctgatcatca tggctatcct tgcgttcccg atgacgtttt ttgctcaccg cggcctgact
    26461 cgcttcgtac tgtctggtaa aaacccgggc gaagacatta ccgaggttgt agaagaacac
    26521 tttggtattg gcgcaggtaa actgattacc ctgctctact tcttcgctat ctacccgatc
    26581 ctgctggttt atagcgtggc aatcaccaat acagttgaaa gcttcatgtc tcaccagctg
    26641 ggtatgacac caccgccgcg tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc
    26701 atcgttcgct ttggtgaaca gatgatcgtt aaagcgatga gtattctggt attcccgttt
    26761 gttggcgtac tgatgctgct ggctctgtac ctgatcccgc agtggaacgg cgcagcactg
    26821 gaaacgctgt ctctggacac tgcatctgca accggaaacg gtctgtggat gaccctgtgg
    26881 ctggcaattc cggtaatggt gttctcgttc aaccactctc cgatcatctc ttctttcgcc
    26941 gttgcgaagc gtgaagagta cggcgatatg gcagaacaga aatgctcgaa gatcctggca
    27001 ttcgcacaca tcatgatggt gctgaccgta atgttcttcg tcttcagctg cgtactgagc
    27061 ctgactccgg cagacctggc tgcggctaaa gagcagaaca tctcgattct gtcttacctg
    27121 gctaaccact ttaacgcacc ggttatcgcg tggatggctc cgattatcgc gattatcgct
    27181 atcaccaaat ccttcctcgg ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg
    27241 attaaatctc tgcgtggtaa aggtaagtct atcgaaatca acaagctgaa ccgtatcact
    27301 gcgctgttca tgctggtaac gacctggatt gttgccaccc tgaacccgag catcctgggt
    27361 atgattgaaa ccctgggcgg cccaatcatc gcgatgatcc tgttcctgat gccgatgtac
    27421 gcaattcaga aagtaccggc aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt
    27481 gtcgtgatgg gtctgattgc aatctccgca atcttctact ctctgttcag ctaagtcctt
    27541 tcgcgccgct ttcgggcggc gcttcctccg ttttaacgcg atgtatttcc tatgattagc
    27601 gtattcgata ttttcaaaat cggcattggc ccttccagtt ctcataccgt tggaccaatg
    27661 aaagcgggta aacaatttac cgacgatctg attgcccgta acctgcttaa agacgtgacc
    27721 cgcgtggtgg ttgacgtgta cggctcgctc tctctgactg gtaaaggcca ccacactgat
    27781 atcgccatta ttatgggcct ggcgggtaac ctgccggata ccgtggatat cgattccatc
    27841 cctggtttta ttcaggatgt gaatactcat ggtcgcctga tgctggcaaa cggtcagcat
    27901 gaagtggagt tcccggttga tcagtgcatg aacttccatg ccgacaacct ttctctgcat
    27961 gaaaacggta tgcgcattac cgcgctggcg ggtgataaag tcgtttacag tcagacttac
    28021 tactctattg gcggtggctt tatcgttgat gaagagcatt ttggccagca ggatagcgca
    28081 ccggttgaag ttccttatcc gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa
    28141 accgggctgt cactctctgg cctgatgatg aaaaacgagc tggcgctgca cagcaaagaa
    28201 gagctggaac agcacctggc gaacgtctgg gaagtgatgt gtggcggtat tgagcgcggt
    28261 atttccaccg aaggtgtgtt acctggcaaa ctgcgtgttc cacgccgtgc tgcggcacta
    28321 cgccggatgc tggtcagcca ggataaaacc accactgacc cgatggcggt tgttgactgg
    28381 atcaacatgt ttgcactggc agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact
    28441 gcgccgacta acggtgcgtg cgggattatc ccggcagtgc tggcgtacta cgataagttt
    28501 atccgcgaag tgaacgctaa ctcactggct cgttacctgc tggtagccag cgccattggt
    28561 tctctttata agatgaacgc gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt
    28621 ggtgtggcgt gctcaatggc ggcggctggt ctggcagaac tgttaggtgc aagcccggcg
    28681 caggtgtgca tcgcggcgga aatcgccatg gagcacaacc tcggtctgac gtgtgacccg
    28741 gtcgccggac aggtacaggt gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg
    28801 gtgaacgccg cacgtatggc gctgcgccgt accagcgagc cgcgcgtctg cctcgataaa
    28861 gttatcgaaa ccatgtacga aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct
    28921 cgcggcggcc tggcaatgaa gatcgttgcc tgcgattaat cgctctccag aggcctcgtt
    28981 ttgcgaggcc tcttcccgat ttctcatcca gccatagcct gttccagtat cgaatgttac
    29041 ccttatcgcc tgatctttaa gggggttatc gtggctgttc atttgcttat tgtcgatgca
    29101 ctgaatctta ttcgtcgcat tcatgccgtt caggggtcgc cctgtgtcga aacctgccag
    29161 catgcgctcg atcagctcat tatgcacagc cagccaaccc acgcggtcgc cgtttttgac
    29221 gatgaaaacc gcagtagcgg ctggcgtcat cagcgtttac cagattacaa agcgggtcga
    29281 ccgccaatgc cggaagagtt gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa
    29341 cgcggcgtcc cgtgctggtc aaccagcggc aacgaagccg atgacttagc cgccacgctg
    29401 gcggtcaaag tgacacaggc cgggcatcag gcaacgattg tttcgacaga taaaggctac
    29461 tgtcagttac tttcaccgac attacgtatt cgtgattact tccagaaacg ttggctggat
    29521 gcgccattta tcgataaaga atttggcgtt caaccgcagc agttgcccga ttactgggga
    29581 cttgcgggga tcagcagttc aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc
    29641 acgcagctgc tggtcgagtt tcagagtctg gaagggatat atgagaatct ggatgcggtt
    29701 gccgaaaagt ggcgcaaaaa attagaaacc cataaagaga tggcgtttct gtgccgcgat
    29761 attgcccgct tacaaaccga tttgcatatc gacggcaatt tacagcaatt gcggttggta
    29821 cggtaacggc gtgccggata cgccgcaaac gtcgcatccg gcattatcac atcagcgcat
    29881 ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc
    29941 accggtacaa acatcatcca gtgccgcctg cgccagtgcc ggaatgtctt ccttgcgtac
    30001 accaacatca cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc
    30061 aacagcggca ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg
    30121 cgcgatatcg cggtacttct caccggtaaa gtcagcgtta taacgcatga catgcggtaa
    30181 caggatggcg tttgcaacac cgtgtggagt gttataaaac gcgcccagtg gatgcgccat
    30241 accatgcacc aaccctaacc ccacattcga gaagcccata cccgcaacat actgcccgag
    30301 cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat
    30361 gatttcaatc gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata
    30421 cccctcaata gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat
    30481 accatccatc atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca
    30541 aacaaacttg cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc
    30601 agtgcctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag
    30661 tccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt
    30721 acaagtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg
    30781 gaatacaccg agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta
    30841 aatcgcccat gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca
    30901 ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc
    30961 atcggttaaa gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct
    31021 gttagccatc atccttctcc ttgttgcttt acgaaattac tcttcaatcc gtaacccata
    31081 ggttttgaat ttctccagca ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc
    31141 cgtaatcgcc agggtcgtca ggtaaagttg cgccagcact tcaacttcat gcgccagcca
    31201 taacgctttt tccagattca cctcacaagc gataagccca tgatgttgta acaaagttgc
    31261 cttacgattt ttgagagcca gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc
    31321 ataaggcgcg caaggaatag aattaccgcc agccgccgca atcatgtagt gaatagcggg
    31381 gatcggtcgg ttaagaatgg aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc
    31441 gttggcatcc ggtctgcttt gataggccgc catatggaaa cgccactcgc ttgaggggag
    31501 ctttccttcc tcatgtttac cgttgccatc aataaagaca atatgcgact ccgtcagttt
    31561 ttcatatgga atgcctgtag gcgtaatcag catcccatcc tgataacgta cactgacgtt
    31621 ccccgctgtc ccctggttca gtcccaggcg ggtcatttcc aggcaagtgt caataatctg
    31681 acgagcaagt ttatttcgtt ccattagcta cctctctctg attcaaaaca gggcaataat
    31741 gttgttcctt tcacactatt gaattagccg tttaattacc caccatcttc ttcctgatta
    31801 acaagaaaga aattcacaag cttatatttt gtgacctagt tcaactaatc acagtaaata
    31861 actgcaagtt ctctttttat aaccccatta aaaatgaccg ctcttaaaaa tatttatcaa
    31921 aa
//
LOCUS       DAZGXS010000042        31680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGXS010000042 DAZGXS010000000
VERSION     DAZGXS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            116..3001
                     /locus_tag="ACHKG5_002092"
     CDS             116..3001
                     /locus_tag="ACHKG5_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0868199.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAILFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTSYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYQLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(3101..5020)
                     /gene="dhaR"
                     /locus_tag="ACHKG5_002093"
     CDS             complement(3101..5020)
                     /gene="dhaR"
                     /locus_tag="ACHKG5_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0868200.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCVTPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5248..6318
                     /gene="dhaK"
                     /locus_tag="ACHKG5_002094"
     CDS             5248..6318
                     /gene="dhaK"
                     /locus_tag="ACHKG5_002094"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0868201.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6329..6961
                     /gene="dhaL"
                     /locus_tag="ACHKG5_002095"
     CDS             6329..6961
                     /gene="dhaL"
                     /locus_tag="ACHKG5_002095"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0868202.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            6972..8390
                     /gene="dhaM"
                     /locus_tag="ACHKG5_002096"
     CDS             6972..8390
                     /gene="dhaM"
                     /locus_tag="ACHKG5_002096"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0868203.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            8710..10394
                     /gene="treA"
                     /locus_tag="ACHKG5_002097"
                     /pseudo
     CDS             8710..10394
                     /gene="treA"
                     /locus_tag="ACHKG5_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            10730..11752
                     /locus_tag="ACHKG5_002098"
     CDS             10730..11752
                     /locus_tag="ACHKG5_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP0868204.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
     gene            11752..12732
                     /locus_tag="ACHKG5_002099"
     CDS             11752..12732
                     /locus_tag="ACHKG5_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0868205.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            12729..13487
                     /locus_tag="ACHKG5_002100"
     CDS             12729..13487
                     /locus_tag="ACHKG5_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0868206.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            13497..14309
                     /locus_tag="ACHKG5_002101"
     CDS             13497..14309
                     /locus_tag="ACHKG5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP0868207.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHLIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            14306..15160
                     /gene="modD"
                     /locus_tag="ACHKG5_002102"
     CDS             14306..15160
                     /gene="modD"
                     /locus_tag="ACHKG5_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP0868208.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            15186..17156
                     /locus_tag="ACHKG5_002103"
     CDS             15186..17156
                     /locus_tag="ACHKG5_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0868209.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(17206..17460)
                     /locus_tag="ACHKG5_002104"
     CDS             complement(17206..17460)
                     /locus_tag="ACHKG5_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0868210.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            17661..18396
                     /locus_tag="ACHKG5_002105"
                     /pseudo
     CDS             17661..18396
                     /locus_tag="ACHKG5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(18398..19009)
                     /gene="emtA"
                     /locus_tag="ACHKG5_002106"
     CDS             complement(18398..19009)
                     /gene="emtA"
                     /locus_tag="ACHKG5_002106"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0868211.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            19109..20023
                     /gene="ldcA"
                     /locus_tag="ACHKG5_002107"
     CDS             19109..20023
                     /gene="ldcA"
                     /locus_tag="ACHKG5_002107"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0868212.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            20119..21855
                     /locus_tag="ACHKG5_002108"
     CDS             20119..21855
                     /locus_tag="ACHKG5_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0868213.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(22248..23318)
                     /gene="dadX"
                     /locus_tag="ACHKG5_002109"
     CDS             complement(22248..23318)
                     /gene="dadX"
                     /locus_tag="ACHKG5_002109"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0868214.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(23328..24626)
                     /gene="dadA"
                     /locus_tag="ACHKG5_002110"
     CDS             complement(23328..24626)
                     /gene="dadA"
                     /locus_tag="ACHKG5_002110"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0868215.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            24989..26521
                     /locus_tag="ACHKG5_002111"
     CDS             24989..26521
                     /locus_tag="ACHKG5_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0868216.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(26573..27292)
                     /gene="fadR"
                     /locus_tag="ACHKG5_002112"
     CDS             complement(26573..27292)
                     /gene="fadR"
                     /locus_tag="ACHKG5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0868217.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            27514..29055
                     /gene="nhaB"
                     /locus_tag="ACHKG5_002113"
     CDS             27514..29055
                     /gene="nhaB"
                     /locus_tag="ACHKG5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0868218.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            29201..29731
                     /gene="dsbB"
                     /locus_tag="ACHKG5_002114"
     CDS             29201..29731
                     /gene="dsbB"
                     /locus_tag="ACHKG5_002114"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0868219.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(29777..31045)
                     /gene="umuC"
                     /locus_tag="ACHKG5_002115"
     CDS             complement(29777..31045)
                     /gene="umuC"
                     /locus_tag="ACHKG5_002115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP0868220.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(31045..31464)
                     /locus_tag="ACHKG5_002116"
     CDS             complement(31045..31464)
                     /locus_tag="ACHKG5_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0868221.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         7731 a         8222 c         7951 g         7776 t
ORIGIN      
        1 agctcatcat gattgcacca tacaaatatc atgatgataa gaggatatta ttcattgact
       61 taaacaaaat gacaaaatcc atagacccat agcctgggta ctatatagga gtggtatggg
      121 catcaaacaa cacaatggga ataccaaagc cgatcgtctc gctgaattaa aaatccgttc
      181 gccctcaatt caactgataa aatttggcgc tattggtttg aatgcaattc tcttttcccc
      241 cctgctgata gctgctgata caggaagtca atatggcacc aatattacta ttaatgatgg
      301 tgacagaatt actggagata ccgccgatcc atcaggaaac ctctatggtg taatgacccc
      361 agcaggaaac acgcctggca atatcaacct gggtaatgat gtcaccgtca atgtcaacga
      421 cgcctctgga tatgcaaaag gaatcattat tcagggcaaa aacagctccc tgacagctaa
      481 ccgactcaca gtagatgttg ttggtcaaac ctctgccatc ggcattaatt taattggtga
      541 ctatacccat gctgacttag gcacaggcag caccattaag agtaacgatg acggcatcat
      601 tattgggcat agctcaacac taacagccac tcaattcacc attgaaaact cgaacggtat
      661 aggcctaacc atcaatgact atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa
      721 gaccgatgga agtacaggtg tttatatcgg tggtctcaac ggcaataacg ccaatggtgc
      781 tgcgcgtttt acggcgacag acctgacaat cgatgttcag ggctacagcg ccatggggat
      841 aaacgtacag aaaaactctg ttgtcgatct cggaacaaac agttccatta aaaccagtgg
      901 cgataatgca cacggcctct ggagctttgg ccaggttagc gcgaatgcac tcactgttga
      961 tgtaactgga gccgcggcca atggcgtcga agttcgtggt ggtacaacca ctatcggtgc
     1021 agatagccat atttcttccg cgcagggcgg tggtctcgtc accagtggtt cagacgcgac
     1081 aatcaatttt tctggcacgg cagcgcaacg aaacagcatc ttttccggcg gttcttatgg
     1141 tgcctcggcc cagacggcaa cggctgttat caacatgcaa aataccgata ttacggttga
     1201 tcgtaatggc agtctggcgc tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga
     1261 cagtttggct atcaccggcg cggcaggagc cagggggatt tatgccatga ccaacagcca
     1321 gatcgacctc acgagcgatc tggtcattga tatgagtaca cccgaccaga tggccatcgc
     1381 aacgcaacat gacgatggtt atgccgccag ccgcatcaac gcctcgggtc gtatgcttat
     1441 caacggtagc gttctttcca aaggtgggct aatcaatctg gatatgcacc ctgggtcggt
     1501 ttggacaggt tcctccctca gcgataatgt caatggcggg aaactggacg ttgcaatgaa
     1561 taacagcgtc tggaacgtaa caagtaattc taatctcgac acgctggcgc tgagccattc
     1621 aactgtcgat tttgccagcc acgggtcaac tgccggcaca tttaccacat taaacgtaga
     1681 gaacctgagc ggtaacagta cctttattat gcgtgctgat gttgttggcg agggtaatgg
     1741 cgttaataat aaaggggatt tattgaatat cagcgggagt agtgctggta atcacgtatt
     1801 ggctatccgc aaccagggca gcgaggccac aacgggaaat gaagttctga cagtggtaaa
     1861 aaccactgac ggtgcggcct cgttcagcgc gtcttctcag gttgagttgg ggggatatct
     1921 gtacgatgtg cgtaaaaatg gcactaactg ggagctttac gcttccggga cagttccgga
     1981 accgactcct aatcctgaac caacaccagc tcccgctcag cctcccatag tcaaccccga
     2041 tcctacgcct gaacccgatc ctacgcctaa ccccactccc acacctaaac ccaccacgac
     2101 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttaaactatg ttgaaaaccg
     2161 tacgctgatg caacgaatgg gtgacctgcg aaatcagagt aaagacggta atatctggct
     2221 gcgcagttat gggggaagcc tggactcctt cgccagtggc aaactgagcg gctttgacat
     2281 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2341 tgtcggtctg tatattggct caacacatgc atcgccggac tatagcggtg gcgacggtac
     2401 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacacaacg gtttttacag
     2461 cgatctcgtt gtaaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2521 caacggcgtt aacgccaacg gcactgcgaa tggattgagc atctccctgg aagccgggca
     2581 gaggttcaac ctgaccccta ctagttatgg gttctatata gagccgcaaa cccagctcac
     2641 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggtctcaata tacatctgaa
     2701 tcactacgaa tcgctgctgg ggcgtgccag catgatactg ggatatgaca tcaccgcagg
     2761 caacagccag ctgaatatct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     2821 agaatatcag ttgaataact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     2881 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctacctcg aagcggatta
     2941 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcact tcagctttta
     3001 agtacggggt agacaagccc ttaaacgatg ctgaaaaatg ttagcgtgcg ccggaatgaa
     3061 atatagatca ctctgatgta tcagaaatcg tctctgtctt tcatacccgg cgcttaaact
     3121 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgattccaa
     3181 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3241 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg tgtggcgctg acatcatctg
     3301 tcgcctgctc ggtaaacaga tgttccggca aatcactgac gcgaatgcgc ccattatcac
     3361 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcca ggccatgcac
     3421 aggaaaccag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3481 caagactgcg taatttgtta ttcaccagag ccggaatgct gccacgccgc atacgcagag
     3541 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3601 gttccaccag cattgcgagg tccgcggtcg ttgtagcaat cactttgaca tcaattggta
     3661 ttaaacgccg cgcatccagt cgcgtgataa caccctgctt gataacctga agcaaagcag
     3721 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgcgcca
     3781 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     3841 ccgccagtgc cgcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     3901 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     3961 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4021 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4081 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4141 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4201 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4261 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4321 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4381 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4441 aactgtccgt cagcagtaaa tttccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4501 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4561 tgctgtcaaa taacggcgtt acacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4621 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4681 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4741 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4801 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     4861 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     4921 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     4981 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5041 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5101 ttcattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5161 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5221 ccctaccgta attgctggag caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5281 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5341 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5401 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5461 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgcgccatg
     5521 caagttgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5581 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5641 attgatgacg acgttgcagt aaaagacagt ctttataccg ccgggcggcg cggcgttgcc
     5701 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5761 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     5821 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta ccctggcgga taatgagatg
     5881 gagtttggcg tcggcattca tggtgagccg ggtattgacc gtcgcccctt ctcttccctt
     5941 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaaa atggctcata ccatcgcact
     6001 ttgcgtttct gggattatca acaaggcagt tggcaggaag aaccacaaac caaacaaccg
     6061 ctccagtctg gcgacggggt gattgcgctg gttaacaatc ttggcgcaac tccactttct
     6121 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6181 gagcgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcact
     6241 ttactgaaag ttgatgacga aacgctggca ctctgggacg ccccggtcca caccccggcc
     6301 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6361 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg gactggatcg
     6421 cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6481 aaaactccct gctattgcag ataaagatat cggtttcatt ctcaagaata ccggtatgac
     6541 gctgctttcc tgcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6601 cgcacaggcg acccaggcac ggcaaagcct gacgctggaa gagctttatc agatgttccg
     6661 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6721 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6781 tccggcggcg ctcgaagctg ccagtagcat cgccgaattc gctgcacaaa gtacgattac
     6841 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     6901 tcccggcgca acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     6961 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7021 tcggtgaatt agcccgtcag atgttaatga gtgataattg taaaatcgcc attgctgcgg
     7081 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7141 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7201 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7261 ctgcgccgtt ggtcgaaggt acactggcag caactgtcag cgcggcctcg ggggcggaga
     7321 tcgacaaagt tatctttgac gccatgcatg cgctggaagc caaacgtgaa caactgggtt
     7381 taccgtcctc cgacactgaa atctctgact catgtcctgc gtacgatgaa gaagcccgtt
     7441 ctctggcggt ggtcattaaa aaccgtaacg gcctgcacgt ccgtccggcc tcccggctgg
     7501 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7561 caccggagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7621 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7681 ataactttgg tgaaacggag gaagttgctc cacctactct gcgtcccgtt ccgcctgttt
     7741 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcacccctga
     7801 ccgtggaaga agaacaggaa cgattacgcc aggccattga cttcacgtta ttagatctga
     7861 tgacgttaac agcaaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     7921 gtcaccatac actgttagat gatccggaac tgctggcggc ggcaagcgaa ctccttcagc
     7981 acgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat
     8041 accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc
     8101 atcgcaccct ggtccacctg acccaaacga aagaagaact cccgcagttt aactcgccaa
     8161 ctattctact ggcggagaac atttatccct ccccagtact gcaactggat ccggcggttg
     8221 taaaaggtat ctgccttagc gccggaagtc cggtatccca cagcgccctg atcgcccgtg
     8281 aactggggat tggctggatt tgccagcagg gtgagaaact gtatgcgata caaccagaag
     8341 aaaagctaac gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa gccaccgttc
     8401 ggatggcatc gttctgatgt catccggcaa tttactcagc actaatgttt tttaactacc
     8461 tgttatattt ttcaccaatt tatgatgatt tttcttcgct aatcgcgaac caaacgacgc
     8521 tggctggcta ataaacagac gttatttgat cactctattt cgccgttatt tatcccttta
     8581 attccctttt ctaaaatgcc tgacagttcg cagaatgaga tttcgatcat gcagctagtg
     8641 cgatcctgaa ctaaggtttt ctgatacttg aataccgttt tattcggttt cgcctaagga
     8701 gaatgattga tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt aattccagcc
     8761 tgcatctttt tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacacc ggtaacgcca
     8821 cagccgcctg atattttatt agggccgctg tttaatgatg tgcaaaacgc caaacttttt
     8881 ccggaccaaa aaacctttgc cgatgccgtg ccgaacagcg atccgctgat gatcggatgc
     8941 agcaaaacca gagcggattt gatctgcgcc atttcgttaa cgtcaatttc accctgccga
     9001 aagagggcga gaaatatgtt ccgccagagg gacagtcact gcgcgaacat attgacggac
     9061 tttggccggt attaacgcgt tctaccgaaa acaccgaaaa atgggattct ctgttaccgc
     9121 tgccggaacc ttatgtcgtg ccaggcggac gttttcgcga ggtatattac tgggacagtt
     9181 acttcaccat gttaggactt gccgaaagcg gtcactggga taaagtcgcg gatatggtgg
     9241 ccaattttgc tcatgaaata gacacttacg gtcatattcc caacggcaac cgcagttact
     9301 atttaagccg ctcgcagccg cccttctttg ccctgatggt agagttactg gcgcagcatg
     9361 aaggcgatgc cgcattgaag caatacctgc cgcaaatgca aaaagaatat gcttactgga
     9421 tggacggtgt tgaaaacctg caagccggac aacaggaaaa acgcgttgta aaacttcagg
     9481 atggtaccct tctcaaccgc tactgggacg atcgcgatac gccacgacca gagtcatggg
     9541 tggaagatat tgccaccgct aaaagcaatc cgaatcgacc tgccactgaa atttaccgcg
     9601 acctgcgctc tgctgctgcg tctggctggg atttcagctc gcgctggatg gacaatccgc
     9661 agcaactaaa taccttacgc accaccagca tcgtaccggt cgatctgaac agcctgatgt
     9721 tcaaaatgga aaaaatcctt gcccgcgcca gcaaagctgc cggagataac gcgatggcaa
     9781 accagtacga aacactggca aatgcccgtc aaaaagggat cgaaaaatac ctgtggaacg
     9841 atcaacaagg ctggtatgcc gattacgacc tgaaaagtca taaagtgcgc aatcagttaa
     9901 ccgcggccgc cctgttccca ctgtacgtca atgcggcagc gaaagatcgc gccaacaaaa
     9961 tggcgacggc gacgaaaact catctgctgc aacccggcgg cctgaacacc acgtcggtga
    10021 aaagtgggca acaatgggat gcgccaaatg gctgggcacc gttacagtgg gtcgcgacag
    10081 aaggattaca aaactacggg caaaaagagg tggcgatgga cattagctgg cacttcctga
    10141 ccaatgttca gcacacctat gaccgggaga aaaagctggt ggaaaaatat gatgtcagca
    10201 ccaccggaac ggggggcggc ggtggcgaat atccattaca ggatggcttt ggctggacca
    10261 atggcgtgac gctgaaaatg ctggatttga tctgcccgaa agagcaaccg tgtgacaatg
    10321 ttccggcgac gcgtccactc agtgaatcaa caacacagcc ggtaaaacaa aaagaggcgg
    10381 aacctacgcc ttaactttgc tgtatatcga gcaatgccat tatgtgcata aaattggcat
    10441 tgctcttttt tcgccacgaa atgcccccca ttctacggtc tatgctttta ttttcttata
    10501 tcagagaaga ttttaatttt attttatttt atcaatttgt taatcagtac acctctattt
    10561 atatttttcg ccactccgcc attctccctg acaattttct tgtggaacaa agtgttattt
    10621 aagcaacaag caaatcattc tgtctcttta gacaaatttt gacatcaaac atgatttttt
    10681 tcgatatata tgtaaatata tagcggtttt aattatcagg atataactta tgtcactaac
    10741 cagacggagg tttacacaga ttcttgcgtc gacgttgttc ctgcatcatc tgccttcttt
    10801 tgcacaatca gtcaaattct gggcctcact gacgcttccc gaagcccaaa acattacacg
    10861 catcgtcagc gcaggtgcgc ccgccgattt gctattgctg gctgtcgcgc cagaaaaaat
    10921 ggtcggtttt tcctcttttg attttgcccg tcaggcatta attccattgt cagagcacat
    10981 tcgccagtta cctaggctgg gacgactcgc cgggcgcgcc agcacactct cgctggaagg
    11041 gcttatggcg ttacatcccg atttggtcgt tgattgcggc aatacggatg aaacctggat
    11101 ctcccaggca cggcaggtta gcgaacagac acaaataccc tggttattgc ttaacgggaa
    11161 actggcgcaa tcagcagaac agttaacaac gcttggcaaa acgttaggcg aagagcatcg
    11221 cgccgcagaa caagccaatc tcgccagccg cttcgttggt gaagctcagg cattcgccgc
    11281 ctcacccgcc gctaacctcc gcttttatgc tgcgcgcggc cctcgagggc tggagacggg
    11341 cttaaagggt tcgttgcata ccgaggcggc ggaattatta ggtttgcaca acgtcgcgca
    11401 aatagccgat cgccaaggcc tgacacaggt ttccatggaa aatctcctgc gctggcagcc
    11461 ggatattatt ctggttcagg aggccgttac tgcagatttt attcgtcgtg atcctctctg
    11521 gcatggcgtg aaagccgttg cagaacaacg catcttattt ttaagtggct tgccctttgg
    11581 ctggctggat gccccaccgg gaatcaaccg tcttctggga ttacgcagac ttcacgcctg
    11641 gctggatccc gccatcaatc gccagtttaa aagtgacatg cagcattacg cccaactgtt
    11701 ctggcattgt tcactcagtg acgccgacta tcaaaaattg gtggcgacct aatgagaatc
    11761 gtaaacggat gtattttgct gctggcagct atcagtatca cttttgccgc cgtaagtggg
    11821 gcttatcatc tcgatatgca gcaactgctg gcgctaattc ttcgtcaaga aaatgttcct
    11881 gtgcaggaac aaattgtctt ctggcaaatc cgcgtgccgc gtattcttgc tgcgctgtta
    11941 ctgggcgcgg cgctggcagg agccggaacc acctatcagg gaatgttgcg taatccctta
    12001 gtttcaccgg atattttagg cgtctccgcc ggtgccggac ttggtgcctg cacggcaatt
    12061 ctgtggggat tatccattgt ttacatccag ctgtacgcat tttgtggcgg gctgatggtg
    12121 gtcgcgggcg tctggttgat tacgcgtcgg gtgacccgcc acgatccgat cttaaccctg
    12181 gtgctggtag gcatcgcgtt aggaacgttg tgcggagcgg ggatatcgct aattaaaacg
    12241 ctggccgatc cttacaccca gttaccgtca attaccttct ggttgctggg gggactgtcc
    12301 accgtgacct tacgcgatct ctgctatgct gcaccgatca ttctcatagg ctccttgccc
    12361 ctgttttttc tccgctggcg catgaattta ctcacgcttt ccgacgacga agcccgttcg
    12421 ctgggactga acgtgacgcg tctgcgcttt gtattgattg tctgcgccac actaatcacc
    12481 gccagtacgg tagcaatagc gggcataatt ggttgggtcg gcctggtcgt tccgcacatt
    12541 gcccgcttgt tgaccggtca taaccatcag cagctgttgc ccatggcaat gtgtactggc
    12601 gcgattctgc tcctgcttac cgatacgctg gcacgcagca ttggaacaac agaaatccca
    12661 cttggcattc tgaccgcttt tgttggcgca ccgtttttcc tgcttttgtt gttacgcgga
    12721 ggtcgccagt gactttgctt gccgtccgtc atgcgtctct gggttactcc cgccatccgg
    12781 tgctacgcga cgtgtccttc accctgtcgc aaggaacaat ttgttgtctg ttaggagcca
    12841 atggttgcgg caaaaccaca ctcatgcgct ccattcttgg cgttataccg ttattaaaag
    12901 gcgaggtctt actggatagc ctccctgtgc agacactcag ccatcgccag cgcgcgcagg
    12961 ctattgcctg ggtgccgcag gcgcacgacg gtatttttgc ttttagtgtg ctggatatgg
    13021 tgttgatggg cctggccccc accataggcg ctttttccgt tcccggtaag caagaacgcc
    13081 tgaaagccac ggagcaactg gaaaagctgg gcattttgca tctggcggca cgacgctgga
    13141 atacgcttag cggcggtgaa cgtcagctgg ctttaatcgc ccgtgcactc gtccagcaac
    13201 cgcgcttgtt gttactggat gagcctgcct ccagcctcga tttcggtcat cagatccaac
    13261 tactggacac gctggcacag ctcaaaaata acggcatgac aatgttaatg tcgactcatc
    13321 atccgctcca tgccaacgcc attgccgaca gcattatcca ggtagagcca gatggacgag
    13381 taacacaagg cttaccgacc gaacagttaa caacaaacaa actggctgcc ctgtatcggg
    13441 tttctgccga ccagattcat catcacctct ctgcaattag ccactaagga aaaatcatgc
    13501 tcattgatga tattgatttt gcagaccttt atctgcagca actcaagctg gcccaccgca
    13561 cagaaaaaac gccggatcac tgggatcaac gggcggaaaa aatggcggaa aattgcgcct
    13621 cgcctacgga cagctacctg caacaactga tcgctaaaat tgacctacaa ggcgctcaga
    13681 ccttattcga tatgggctgc ggaccgggta ccgtatcgct ggcgctggct gacaaactca
    13741 cgacgattta tggcgttgat tacagtcagg ggatgctgaa cgttgccgcc cgtcgcgccg
    13801 ccgcgctgaa ggcagacaat gtccacttga tccagcgcgc ctgggaagag gactggagtg
    13861 acctgccacg ctgcgatatt gccgtggctt cccgctccac tctggtggct gatatgcgcc
    13921 aggcaatgag caaactgaat aatcaggcac ggcttcgcgt ctatacgact catctggtca
    13981 gcacctcgtt cgtctctcca gccattcagc gggcggctgg tcgggaagtg atcgaattac
    14041 ccaactatat ctttgcgctc aacgtcctgt atcagatggg aatttatgcc cacgttgatt
    14101 ttatccgtgg acaaaattgc caacaggata acagcacctg ggaacgtttt gagcagaacg
    14161 tcagctggag tcttggcgcg cttaacgacg atgagcgtga gcggttatat cgctggtatc
    14221 aacaacaaga tgctcgtgcc ctcgcccctg ccagccgcga ctgggcgctc atctggtggg
    14281 atagcgtacc gcaggaggca ctgcgatgat tttcctttct caggcacaaa tcgatgcgtt
    14341 acttctggaa gatatccagg gcggtgacct gaccacgaga gcattaaata ttggacacca
    14401 gcatggctat atagagtttt ttctccgtca gggcggctgc gtcagcggcg tttctgtcgc
    14461 gtgtaagatg ttaactacgc tgggattaac tattgatgac gcggtcagcg acggttcaca
    14521 agcgaacgcg ggtcagcggc taatccgtgc gcagggtaat gccgcagcac ttcatcaggg
    14581 atggaaggca atccagaatg tgctggagtg gagttgcggt gtttctgatt atctcgatca
    14641 aatgctggcg ttacttcgtg aacgttatcc tgatggcaat atcgcctgca cccgaaaagc
    14701 aattccgggc acccgcttac tggcctcgca ggcaattctg gctgccggag gactgattca
    14761 tcgtgccgga tgtgcggaaa ccatattatt gtttgccaac caccgccatt ttcttcatga
    14821 caatcaggac tggtcaggcg caatcaatca gttacgtcgc cacgcacccg agaagaaaat
    14881 tgttgttgaa gccgacacgc cgaaagaggc aatcgccgcg ttacgcgcgc aaccagacgt
    14941 acttcagctc gacaaattta gtccgcaaca ggcaactgaa attgcgcaaa tagcgccgtc
    15001 gctggctccc cactgcacgc tggctctgac cggcggaatt aatctgacga cactcaaaaa
    15061 ttacctcgac tgcggcattc gcctctttat tacctccgcg ccttattacg cggcaccggc
    15121 tgacattaaa gtcagtctgc aacccgcagc ctctatttaa ttctttgtta aacggaattt
    15181 attctatgag actcaaaaaa cattatctct gcacagcatt atcacttgcc tttacccagc
    15241 aggccgtagc ggctcaggag agcgactcgc tgaccgtatg gtccagtccg gtatcatcga
    15301 cgacgaccac cgttctcgat caacccacca tgaaggccct ggataaacag aatgtcgctc
    15361 aggcattaag tgtcgtcccc ggcgtggtgc tgcaaaagtc aggcagccgc aacgaagaac
    15421 aggtaaaagt tcgtggcttt gatagtcggc aggtgccggt ctatttcgac ggtgtgccca
    15481 tttatgttcc ctatgacggc aacctcgatc tggcgcggat tctgaccaac aatctggggg
    15541 cagtcgaagt ttccaaaggg tattcgtcgc tgcttcaggg acctaatcag atgggcggag
    15601 ccattaatat caccacccag aagccaacaa aacctctgga agcaagtctg ggatatcgcc
    15661 agggatggag ccgtagccag gacaatgcct atgatatgca tgcttcattt gccgccagca
    15721 gcgaactggg gtatttgcaa gtcagcggta gccagctaaa gcaggatttt ctcggcctgc
    15781 cgcatggtgt aaataatgat attgcaggca aacacggcaa gatgattaat tcatcggctg
    15841 atgataaacg cggcattgtg aagctcggat ttacaccacg tgaaaacgat gaatacacac
    15901 tgacttacat taagcaggat ggtgaaaaag ataacccgcc atacagcgga aatagtggtc
    15961 aaaaatcacg ctactggcag tggccagagt atgacaaaga aagtttttat tatcagggaa
    16021 cgacccaact aaacgatcgt tttaccctga aaagtcggct gtatcgcgac acctttgaaa
    16081 atacgctgat gatgtacaac tcgctggctg atttgaaaaa taaaaaaggc agctacagcc
    16141 attattccga ttacagcgac ggtgccggat tacaactggc agccgatgtg cgtgaaaacg
    16201 atctgctgac gtttgccgtt aactggaaag atgacgtaca ccgggaaaaa ggtgcgccgc
    16261 acgccgctta cgatcgctat gaagatcgta cctggtcgct cgccagtgaa tatcaatggg
    16321 ctgctgccga taatgtcgat gttgtggctg gaatcagcta tgactggcgc gatagcgtag
    16381 aagcgaaaaa acacgagaaa gatggcagca tcacccatta tgacgacaac aatcagtcag
    16441 cttttaactg gcaggtgatg gggaaatacc actttgtcaa tgaagacacg ctggcgcttt
    16501 cgtactatga ccgcaaacgc tttccgacgc tgaaagaacg ctataccaca tccaaacctg
    16561 cgtataacca gatagcgatt gttaacccgc agctcaagcc ggaacgcgct cgcggggtgg
    16621 atttaacctg gaatggtgcc ttcacgcacg actggggatt tgaggtcagc gtttactata
    16681 accgggtgag tgatgccatc ctctcgcaca atatcgatgc cgataccatt caaaatcaga
    16741 acagcggcac ggtggattac agcggtctgg atgccggtat taaggggaaa atcagcaata
    16801 tactggatgt aggattgagc tacgccctga tccacgctga cgccaaacgt aaagacatcg
    16861 gcaagataac cgatctgcca acgcagacaa tgaccgcatg gatgactctc aaaccgtggg
    16921 agccgttaag cgtaacgctg tcggaagagg cgcgttcctc cagctacagt aacagtgacg
    16981 gttcacaaaa agccgccggt tttgcggtga cccacattcg agccgattac accttaggtc
    17041 atggcttcag cgtcaatgcg tcggtcaata acctgtttga tacccaatat gcctacagtg
    17101 aagggtttgt tgaagaaggt cgcaatttct gggcaggtgt cgagtatacg ttctgatgtg
    17161 aaagcggata gcggcatctg tatcgctatc cgccttatga aaatcttatt ctcttcgcag
    17221 gaggcggaat acgcccagaa cgagaatagc tcccaccacc gccaccagga agctgtgcaa
    17281 attaaaaccg ctgatggagc cgccaatgcc aaacatggtc gccagccagc cgccgaccac
    17341 cgcaccgact atcccgagaa tacaggtcag gaaaaatcca ccaccatcac gccccggcat
    17401 gattagcttg gcgataatgc cggctatcag gccaaaaata atccaggcaa taattcccat
    17461 gttcaggccc tcgtttcgat gaatcggacg caagaaatgc ttacgtcaac gctctaagta
    17521 taggcaaggg cgggatcgat gctgggcgta tgtcggctga tttaagaaca acatgtaaaa
    17581 aatattcacg catttgtatt aagttttgtt aactgtgacc gataaaccaa agacagtttg
    17641 tcagtcagga gtttttccgc gtgagtcatt accatgagca gttcctgaaa caaaatccgt
    17701 tagccgtcct gggcgtgtta cgcgatttgc acaaagccgc aattcctttg cgtctcagtt
    17761 ggattggcgg gcagctgatc agcaaaatac tggtaataac cccggataaa ctggtgctgg
    17821 atttcggcag tcaagccgaa gacaacatcg ccgtgctaaa ggcacagcac attaccatta
    17881 ccgccgaaac tcagggtgcg aaagtcgagt ttactgttga acaactacag cagagtgaat
    17941 acttgcagct tccggcattt attaccgtac cgcctcccac cttatggttt gtacaacgac
    18001 gccgatattt ccgcatctcc gccccactcc atccgcctta tttttgccag accaaactgg
    18061 cggataacag tacgttacgt ttccgcctgt atgatttgtc gttaggcggc atgggcgcat
    18121 tactggaaac agcaaaacct gccgggttgc acgaaggtat gcgcttcgct cagattgaag
    18181 tcaacatggg gcaatggggt gtttttcact ttgacgccca gttaatctcc atcagcgagc
    18241 gcaaagtgat tgattggcaa gaatgaaacc atcaccactc cccgtctgag cttccgtttt
    18301 cttaacgtca gcccgacggt ggagcggcaa ttacagcgga ttattttctc tctcgagcgg
    18361 gaagcccggg agaaagcgga caaagtacgc gactgattta catcgcgtcc agtgcctgct
    18421 caagtttgta gatatagcgc ggagcctgcg gcgcaggatg atttcgcgct acgtgatcga
    18481 ggaactcgtc agcatccaga tcgttgattt tgctgattgc ctttttccga tctgacgaga
    18541 aagtccgtag cagcgcacct gccccgttag catatgacac caccagcgca tattgcagta
    18601 ccttcggatc ttcaatgcct gccagcgggc cggtttccag aatattcagg taagccgccc
    18661 ccatagaaat attacgctcc ggatttttca gctcgctggt cgtcggctca ccactccagc
    18721 ccatacgccg ataaacatca cgtccggagg ttgaagcttt taactgcatc aaaccaatgg
    18781 cattcgattt actcaccgcg ttcggattac cacccgattc gatagcgata atcgccgtga
    18841 tcaattgtgg atcgacgccc caggctgcac cggctttctg gcttattggc atccactgca
    18901 tcgcacgttg caccggaact ttcgcgttcc acggcgggtt cgtatagtca tgctttgatg
    18961 aacaacccgc taataacaca atcaaaaagg caaaccatct caatttcact tcttctatcc
    19021 ttatagcctg gaatcgccgt tgaggtgaca acggcacacc taagcggcat gatatacatt
    19081 tcgtatctgt tgtcagcaag gaattgccat gtctctgttt cacttaattg ccccatcggg
    19141 ttactgcatt aaacagcacg ccgcgctgcg tggtatccag cgccttaccg acgcggggca
    19201 tcaagtcaat aacgtcgagg ttattgcccg tcgttgtgag cgttttgcgg gtacggaaac
    19261 ggagcgtctg gaagatctca attcccttgc cagactgact acccccaaca ccatcgtgct
    19321 ggctgtacgc ggcggttacg gtgccagtcg tttactggca gatattgact ggcaggcact
    19381 ggtggctcgc caacaatatg acccgttgct catatgcggg catagcgatt ttaccgccat
    19441 tcagtgcggt cttctggcgc aaggcaatgt catcaccttt agcggcccca tgctggtagc
    19501 aaactttggc gcggatgaac tgaacacctt taccgagcac catttctggc tggcgttacg
    19561 caatgaaacc tttaccattg aatggcaagg ggaaggcccg acatgtcggg ccgaaggcac
    19621 gttgtgggga ggcaatcttg cgatgctgat ttcactgatc ggtacaccgt ggatgccaaa
    19681 aattgagaac ggtattctgg tgctggaaga tattaacgag caccctttcc gcgtcgaacg
    19741 tatgctgttg cagctctatc atgcgggaat tttgccacgt cagaaggcga ttattctcgg
    19801 tagctttagc ggcagtacgc ctaatgatta tgacgcaggt tacaacctgg agtcagtgta
    19861 cgcatttttg cgttcccgcc tgtcgattcc actcattacc ggcctcgatt ttggtcatga
    19921 acaacgcacg gttacgctgc cgttgggcgc acatgccatt cttactaaca cccgggaagg
    19981 cactcagttg acgattagcg gtcatcctgt tcttaaaatg taaaaaaaat cacgcttcag
    20041 ggctaagtaa aacgctgtct ctgccgctaa tatggtaggg tttatataag agacagcgta
    20101 atcaggagta accgaccgtt ggatgccaca acaataatta gccttttcat cttaggatcc
    20161 atccttgtta ccagcagtat attacttagt tcattttctt ctcgtcttgg cattcctatt
    20221 ctggttattt ttctggcgat cggcatgctg gcgggagtcg atggcgtcgg cggtatccca
    20281 tttgataatt accccttcgc ctacatggta agtaacctgg cgctggcgat tattttgctc
    20341 gatggcggga tgcgcactca ggccagttcc tttcgtgtgg cgttaggacc ggcgctgtcg
    20401 ctggcgacgc tgggcgtact tatcacctct ggtttaaccg gcatgatggc ggcgtggctg
    20461 tttaatcttg atttgattga aggcttatta atcggcgcta tcgtcggctc caccgatgct
    20521 gcagcggtct tttctctgct gggtggtaag gggcttaacg aacgtgttgg ctcgacgctg
    20581 gaaattgaat ccggcagtaa tgatccaatg gcggtctttc tgacgattac cctgattgcg
    20641 atgatccagc agcatgaaag cagtgtcagt tggatgttcg tggtcgatat tctgcagcaa
    20701 tttggcctcg ggattgtcat cggtcttggc ggtggttatt tactgctgca aatgattaat
    20761 cgcatcgccc tgcccgccgg tttatatcca ttgctggcat taagcggcgg tattttaatt
    20821 tttgctttaa ccacggcgct ggaaggtagc ggtattctgg ctgtttatct gtgcggtttt
    20881 ctgctgggta atcggccgat tcgcaaccgc tacggcatcc tgcaaaattt cgacggcctc
    20941 gcctggctgg cgcaaatcgc catgttcctg gtgctggggc tgttggttaa cccaagcgat
    21001 ctgctgccta ttgccattcc ggcgctcatt ttgtccgcat ggatgatttt cttcgcccgt
    21061 cctctttcgg tatttgccgg attgctacca ttccgtggct tcaatctgcg tgagcgcgtg
    21121 tttatcagct gggtgggatt acgcggcgcg gtgccgatta ttctggccgt gttcccgatg
    21181 atggcggggc tggagaatgc acgtctgttc tttaatgtcg ccttctttgt ggttctggtt
    21241 tcactgctct tgcagggaac atcactctcg tgggcggcga aaaaagccaa agtggtggtt
    21301 ccgcccgtcg gacgtccggt gtcacgcgtt ggcctggata ttcatccgga aaatccgtgg
    21361 gagcagtttg tttatcaatt gagtgccgat aaatggtgcg tgggcgcggc actgcgcgat
    21421 ttgcatatgc caaaagagac gcgtattgcg gcactgtttc gtgataacca gttgcttcat
    21481 cctaccggca gcacccgact gcgcgaaggc gatgtgttgt gtgtaattgg tcgggaacgc
    21541 gatctcccgg cgctcggtaa actgttcagc cagtcgccgc cggtcgcgct ggatcaacgc
    21601 ttctttggtg acttcattct cgaagccagc gccaaatatg cagatgtggc gctgatatat
    21661 ggcctggaag acgggcgaga atatcgtgat aagcaacaaa cgctgggtga aattgtccag
    21721 cagttgttag gcgcggcacc ggttgtcggt gaccaggtag agtttgccgg gatgatctgg
    21781 acggtggccg agaaagaaga taatgaagtg ttgaagattg gcgttcgagt ggctgaagag
    21841 gaagctgaat cttaaattta atgaagcatc tatcgccggt tacgatgctt tgcttaacgc
    21901 agcctgagcc tgtatataaa cttgtcattt cctgattttg atatgcacat tccaacatca
    21961 gaagaaggca gcctgttaat caacattctg aacatgaacc cgaatctgca tcaagggtga
    22021 agcgtgattt atctgaagtc gagttcgagt caacaatgaa gcgtaagcgt tttatgtaga
    22081 acgcctgtgc ggtgagttca ggttccgggg aaatagtgtc catcctttta atgggcacta
    22141 tcatgggcaa tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat
    22201 tttttttgca accagaagac gttgcctccg atccgactta caacaagtta caccgtcaca
    22261 accgggacgc gtaacgccag cgcgcacatc aactcatagc ccaccgttcc ggcagcggcg
    22321 gcgacatcat caattttgat ctccttgccc cacagctcaa ctggcgtacc aatacccgcc
    22381 tgcgggcaag gcgttaaatc gaccgccagc atatccatcg agactgtccc caccgtcatg
    22441 gtgcgcacgc cgtccactaa aacaggggta ccggtaggcg cgtggcgcgg ataaccgtcg
    22501 gcgtatcctg cggcaacaat gccgattcgc tgctcatcgc gcgcagtata acgaccgccg
    22561 tagcccacac gctcgcccgc ttttagcgtc tggacaccaa taatctcact gcttagcgtc
    22621 atcaccggac gtaatccagt attggcgata tcacgccact gaccggacgg cgaagcgcca
    22681 tacaaaataa tgccaggccg aacccagtca aaatgcgctt ctgggtgcca cagagtcgcc
    22741 gccgaattgg acaacgaacg ccgacactcc agcccttccg ccgcctgctc aatacgcgcc
    22801 atcgcgccgg aaattccatc aggatgttcc gcctcggcaa aatgcgacat cagggtcatt
    22861 tcgccaacat tcgccatcgc ccgcaactgc tgccagacgg taagcacgcg atcgggctgg
    22921 aagcccaacc gattcatccc actgttcact ttaagataaa tatccaacgg tgcttttagc
    22981 cgcgcatttt gcagtgcttt gagctgccag ttgctgtgta cgcaggtggt caggcggtgc
    23041 tggtcataca tttccagatc ctgagcatgg aaaaatcctt ccagcatcag gatcggtcct
    23101 ttccagccgc gctcacgtaa cgttattgct tcttccaggt taagtaatgc aaagccatcg
    23161 gtagccccga gcgcgctcca gatacgctca ataccgtgcc cgtaagcgtt cgcttttacc
    23221 accgaccaga cgcgcgcgtg cggcgcggcc tggcggacaa tggacagatt ctgttttaat
    23281 gcctgcagat cgaggctggc ctgtatcgga cgggtcatct cgtttcctta gctgtgtgcg
    23341 ccatgtaaat ggcccggacg tgatggcgta aatccacggc tgtagcgcgc tacgcttaga
    23401 tcctcatatg ggatcgctgg cgtgcgacca gagagcagat cgcttaacaa ctgaccggaa
    23461 ccgcaagcca tcgtccagcc gagcgtgccg tgaccggtat ttagccacag atttttaaag
    23521 cgtgtacgcc cgacaaccgg cgtgccgtct ggcgtcatcg ggcgcagacc cgtccagaaa
    23581 gtcgcctgct cgacatgacc gccgcgtgga tagagatcgc gaaccaccat ctccagcgtt
    23641 tcacgacgcg gttgcaacag ctcggtatta aaaccaacaa tttccgccat tccgccaaca
    23701 cgaatgcggt tatcgaaacg ggtaatggcg attttgtagg tttcatcaag aatggtggat
    23761 accggcgcac catcttcctg cgcaattgga atggtcagcg agtagccttt cagcggatac
    23821 accggaatat caacaatgcc tttgagcatc gccgtcgagt aagaaccaaa cgccatcaca
    23881 tacgcatcgg ccttaatcac ttcatcgcca cacttcacgc cgtagatttg ctcgccgtcg
    23941 caaagcagtt ggtcaacggg cgtattaaag cggaatttaa cccccgcctg ctccgccatc
    24001 cgcgccagat tctgggtaaa cagctggcag tctccggttt catcattggg caattgcaga
    24061 ccacccgtca gtttgtgcgc tacttctgcc agcgcgggct ccacttccgc caggcggctg
    24121 gattccagca gctgatacgg tacgccggca tcttccagca cggcgatatc gcgggtcgca
    24181 ttttcatact gttgttcggt acggaacagt tgcagcgtcc caccctgacg cccttcatac
    24241 tgaatattgg tttcggcgcg caatgctttc aggcaatcac ggctgtattc cgccagacgc
    24301 accatccgcc ctttgttttc catgtagtgg ctggtgtcgc agttacgtaa catttgccac
    24361 atccatttca actggaactg cgtaccgtcg agacgaaccg ccagcggcgc atggcgctgg
    24421 aacatccatt taatcgcttt taaaggcaca cctggtgccg cccacggtgc agcatatccg
    24481 ggggagattt gcccggcatt cgcggcactg gtttccaggg cggcccccgg ctcccgatca
    24541 atgacggtga cctcatgtcc tgcctgattt aagtaccagg cgctggcaac gcctaccaca
    24601 ccacttccca gtatgacaac tcgcatagcc actccgataa cggtagatac agtaaaagaa
    24661 taataatcta attacatctt gataactcag ttgaaaatat tattcaacat acggcttttt
    24721 tatggtgagc tggctcacat ctccctgact ctgcggcaat atggcgtctt tagtatctcc
    24781 tgctgaacgc cctttttatc cagcataaaa ttccgttcag aaacggatta gtggcactct
    24841 gataataaga aatcgcaaag aaaaaaattc tccggcgacg cggtttttaa caggtgttct
    24901 atgcttgaaa tgaggtgctc cacacagaga gcgccgacaa caatgaggtg ctccacacag
    24961 agagcgccga caacaatgag gtgcgcgtat ggcgacgatc gattctatga ataaggacac
    25021 cacacgtttg agcgatggac ccgactggac gttcgacctg ctggatgttt atctggcaga
    25081 gatagaccgg gtggcgaaac tctaccggct ggatacctac ccgcaccaga ttgaagtgat
    25141 aacctcagaa cagatgatgg atgcctactc cagcgtcggc atgccaatta actatccgca
    25201 ctggtcattc ggtaaaaagt ttatcgagac tgaacggctg tataagcacg gtcagcaagg
    25261 actggcctat gaaatcgtca ttaactctaa cccgtgtatc gcttacctga tggaagagaa
    25321 caccattacc atgcaagcgc tggtgatggc tcatgcctgc tatgggcata actctttctt
    25381 caaaaacaat tacttattcc gtagctggac cgacgccagt tcgattgtcg attatctgat
    25441 ttttgcccgt aaatatatta ccgagtgcga agaacgttat ggcgttgatg aagtagaacg
    25501 gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg gaccggtaca aacgcccgca
    25561 aaaaatctcg ctgcaagaag agaaagcccg gcagaaaagt cgcgaagagt atttgcaaag
    25621 tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag gaagagaaaa cggttgctga
    25681 agcgcgccgt tatccgtccg aaccacaaga aaacctgctc tattttatgg agaaaaatgc
    25741 gccactgctg gaatcatggc agcgtgaaat cctgcgtatt gtgcgtaagg tgagccagta
    25801 tttttatccg caaaaacaga ctcaggtgat gaacgaaggc tgggccacct tctggcacta
    25861 caccatcctt aaccatctgt atgatgaagg gaaagtaacg gaacgtttta tgctggagtt
    25921 tttgcacagc cacaccaatg tggtcttcca gcccccctat aacagcccgt ggtacagcgg
    25981 catcaacccg tatgccctcg ggttcgccat gttccaggat attaaacgga tttgtcagtc
    26041 gccaacggaa gaagacaaat actggttccc ggatatcgcc ggttccgact ggctggaaac
    26101 gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt atcagccagt tcctgtcgcc
    26161 gaaagtgatg cgtgatttcc gcttcttcac cgtgctggat gatgatcggc ataattatct
    26221 ggagatttcc gctattcata atgaagaagg ttatcgggag atccgtaacc ggctatcgtc
    26281 gcaatataac ttaagtaatc tggagccgaa tattcagatc tggaacgtgg atttgcgcgg
    26341 cgaccgttcg ctgacgctgc gttacattcc acataatcgc gcaccgctgg atcgggggcg
    26401 caaagaagta ctgaagcatg tgcatcgcct gtggggattt gatgtgatgc tggaacagca
    26461 aaacgaagac ggcagcgtcg agttgctgga acgttgcccg ccgagaatgg gaaatctgta
    26521 aaaacaacaa aaaacccctc gtttgagggg tttgtttttt aaacggaagg gattatcgcc
    26581 cctgaatggc taaatcaccc ggcagatttt tctgcatccg gtgccaaatc tcgccactct
    26641 catgcccata gcgacgcact gtttcgtaca cctgatcgtg cgcgccttca ctgcacaacg
    26701 ccgacagttt gtggtagaag cccagcgcca gactgcgcgc ttccggattg gcgaaatagt
    26761 gacgaccaat gcgcgtatac agtcccttca tcccgttaag gatcagaccg taaatcgggt
    26821 tgccggaagc aaacgccagg ccgcggaata tgttgtaatc cagctcggca aaggcatcgg
    26881 cgtgatcggc cacttcatta gcggtagcca gcacttcctg cgctttatcg ggatgctgac
    26941 gaaacgcggt gcgaataaaa atagttgaaa tattggtacg caccgacagc aaattatcaa
    27001 taagctgcgg cacactttcg tgatccagtc gcgccagtgt ttcaaggata tttaaaccag
    27061 aggtttccca gaaattattc accttcgtcg gcttgccatg ttgaatggtc aaccagccat
    27121 ctcgtgccag acgctgtaac acttcacgta acgtagtacg cgttacgcca attaattctg
    27181 aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa gcggttattc cagatacttt
    27241 caataatgta ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc atagtgagat
    27301 ttccataaca cagcaaaaca aagttggact catcatacca gagggctaac tacgctgata
    27361 gcagacaaaa agaagaaaaa agggatcaag gcttcatttt ccgcatctct ttccccttga
    27421 ctatcaagac ttgcctcact cctgcctgac cgttaagctt tgcgtttaaa cataaaaaca
    27481 tcattgatta tttgtaaggt aagggaaacc atcatggaga tctcctgggg ccgcgcgcta
    27541 tggcgcaatt ttttgggcca gtcccccgac tggtacaaac tcgccctcat cattttctta
    27601 atcgtaaatc cgttaatttt cctcatcagc cctttcgtcg ctggctggtt gctggtagcg
    27661 gaatttattt tcactctggc gatggccctg aaatgctacc cgctgctccc cggtggcctg
    27721 ttggctatcg aagcggtatt catcggcatg accagcgcgg aacacgtccg tgaagaggtg
    27781 gcggcaaatc ttgaagtctt gctgttactg atgtttatgg tggcgggtat ctattttatg
    27841 aaacagctat tgctgttcat atttacccgt ttgctgttaa gcattcgctc caaaatgctg
    27901 ctgtcgctct ctttttgcgt agcggcagcg ttcctctccg cgttcctcga tgccttaacc
    27961 gtcgtggcgg tggtgatcag cgttgccgtc ggtttttatg gtatttatca tcgcgtagcc
    28021 tcttcccgta ccgaagacac cgacctgcaa gacgatagtc atatcgacaa gcattacaaa
    28081 gtggttctgg aacagttccg tggctttctg cgtagcctga tgatgcatgc cggtgtcggc
    28141 accgcattag gcggcgtaat gaccatggtg ggcgaaccac agaacctgat catcgctaaa
    28201 gcggctggct ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac cgttccggtt
    28261 ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt tggctacggt
    28321 gaaacgctgc cggagaaagt ccgcgaagtg ttgcaacagt ttgacgatca aagccgccac
    28381 cagcgtaccc gtcaggataa aatccgtctg attgtccagg cgattattgg cgtctggctg
    28441 gtgactgcgc tggcgttgca tctggcggaa gttggcttga ttggtttgtc agtcattatt
    28501 ctggcaacat cattgaccgg tgtcaccgat gaacatgcta tcggtaaagc cttcaccgaa
    28561 tctctgccat tcaccgcact gttgacggtg tttttctcgg tagtcgcggt gattatcgac
    28621 caacaactgt tttcgccgat tattcagttt gtgttgcagg catcggaaca tgctcagctg
    28681 tcgctgttct atattttcaa cggtctgctg tcatccattt cggataacgt cttcgtgggg
    28741 acgatttata tcaacgaagc gaaagcggca atggaaagtg gcgctatcac gttgaagcaa
    28801 tacgagctgc tggcggtcgc cattaatacc ggtaccaatc tgccctctgt cgctacgccg
    28861 aacggtcagg ctgcgttcct gttcctgctg acctctgcac tcgcgccatt gattcgcctc
    28921 tcttatggcc gcatggtgtg gatggccctg ccttacaccc tcgtcctgac actcgtcgga
    28981 ctgctctgcg tcgagtttac gcttgcccct gtaaccgaat ggtttatgca aatgggctgg
    29041 atagcaacgc tttgataaca acttaccggg catataaatg cccggtttgc cttttcgccc
    29101 gataattgtc caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg aatgaattgg
    29161 tttaaactgc gcactctatg catattgcag ggaaatgatt atgttacgat ttttgaacca
    29221 atgttcacaa ggccggggcg cgtggctgtt gatggcgttt actgctctgg cactggaact
    29281 gacggcgctg tggttccagc atgtgatgtt actgaaacct tgcgtgctct gtatttatga
    29341 acgctgcgca ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg ccccaaaaac
    29401 gccactgcgt tatgtagcga tggttatctg gttgtatagt gcgttccgcg gtgtgcagtt
    29461 aacttacgag cacaccatgc ttcagctcta tccttcgccg tttgccacct gtgattttat
    29521 ggttcgtttc ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt ttgtcgcctc
    29581 tggcgattgc gccgagcgtc agtgggattt tttaggtctg gaaatgccgc agtggctgct
    29641 cggtattttt atcgcttacc tgattgtcgc ggtgctggtg gtgatttccc agccgtttaa
    29701 agcgaaaaaa cgtgacctgt tcggtcgcta atccattcgg cgctcctgcg ggagcgcttt
    29761 tttcctgccg ctgtgtttat ttgacccgca gtaaatcaga acttcgcgtt gtataacgtg
    29821 gtgaaagcat ggctcgcttc atctgccatt gttgctggat cccctgcccg gcaaaataga
    29881 gtgttcctct gccctcttta gcattcagtg tatccattac cgccatcaat tgctcactcc
    29941 cggggcgcgg agcgttgtca tcgaataaat tgagctgcgc gactccctga ctgaagaaat
    30001 cccccagcat cacgcccgct ttttggtagc gatggcccgc ttgccagatg gcatccagag
    30061 atcgcgtagc ggcgttaatg atatccctgc tgtcctgagt gggcgtcagc agttttaccg
    30121 acgcgctatt gccgtaataa ggttcattga gcgcaaatgg tgacgtctta ataaatgtgg
    30181 agataaaccg acaatattga tgctcactgc gaagtttttc cgccgcccgg gcagcgtaac
    30241 tacaaatggc ctgccgcatc gacgtataat ccgtgatgcg ttcaccaaac gagcgggaac
    30301 agataatttc ctgctttgtc ggtgcaaatt cttccagttg caaacagggt tcaccgcgca
    30361 gttcacgcac cgttctttcg agcacgacat taaaatgttt acggataaac cggatatctg
    30421 tatccgccaa atcgagaacg gttttgatcc ccatcgcgtc cagttttttg ctgatccgcc
    30481 gtccaatccc ccagacgtca tccacgggga gagcagacat taatttacgc tggcgttcca
    30541 gatttgataa atccaccacc ccacccgtct gccgctgcca tttttttgcc gcatgattgg
    30601 caagcttagc cagcgttttg gtctgggcga tccccacacc aacagtaaga tgggtacgtt
    30661 gtagcaccgt tgcgcgaatt tctctgccaa agtcagtcag atcgcgacaa ttacgcacac
    30721 ctgtcagatc gcagaatgcc tcatcaatac tgtaaatctc gacgcggggc gatagttctt
    30781 ccagcgtcga catcacccga ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc
    30841 aaaccacgcc acagcgacga aacagatctt tttgtttgaa ccagggatcg cccattttaa
    30901 cgccaagcgc ctttgcctca gcgtttcggg cgataacgca accgtcatta ttcgatagca
    30961 caaccaccgg tttaccccat aactcagggc gaaacaccgt ctcacagctg gcataaaacg
    31021 cgtttacatc acagagggca aacatcagcg catcgcctta acgacgtgga tcaccacacc
    31081 aaagacatcc agcgtatctt cactactgat ggtaatgggc gagtacgcgc tgttcatggg
    31141 aataagctgt accgtcgggc gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc
    31201 agcgatgaca atatcaccat ggctggcggt aatagcgcta tcgacaatca gtaaatcacc
    31261 gtcactaatt ccaccatcaa tcatagaatc accacttgct ttgacgaagt aagtcgcgct
    31321 gggatgctgg atcaacagtt gattcagatc gatgcgctgt tcaacgtaat ctgcggccgg
    31381 tgaaggaaag ccacactgaa caagatcgct aaatagcgga aaagtcacaa tttcgcggag
    31441 atccgcaggc ttgataaaca acataataat ctgcctgaag ttatactgtt tttatataca
    31501 gtagtctgtt cttgccagca gatcaatact gattcaggct atcaatattt gtcgctgcat
    31561 agactgctga tttttcgttc tcttatcctg tgctcacgcg gccttctggc gacgacgctc
    31621 atccagcaga aatgaaaaat atcacccggc taaaaaatag aatagaagca ttcgccataa
//
LOCUS       DAZGXS010000043        31362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGXS010000043 DAZGXS010000000
VERSION     DAZGXS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..599
                     /locus_tag="ACHKG5_002117"
     CDS             <1..599
                     /locus_tag="ACHKG5_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP0868222.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            829..1212
                     /gene="secE"
                     /locus_tag="ACHKG5_002118"
     CDS             829..1212
                     /gene="secE"
                     /locus_tag="ACHKG5_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0868223.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1214..1759
                     /gene="nusG"
                     /locus_tag="ACHKG5_002119"
     CDS             1214..1759
                     /gene="nusG"
                     /locus_tag="ACHKG5_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0868224.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1918..2346
                     /gene="rplK"
                     /locus_tag="ACHKG5_002120"
     CDS             1918..2346
                     /gene="rplK"
                     /locus_tag="ACHKG5_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0868225.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2350..3054
                     /gene="rplA"
                     /locus_tag="ACHKG5_002121"
     CDS             2350..3054
                     /gene="rplA"
                     /locus_tag="ACHKG5_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0868226.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHKG5_002122"
     CDS             3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHKG5_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0868227.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3910..4275
                     /gene="rplL"
                     /locus_tag="ACHKG5_002123"
     CDS             3910..4275
                     /gene="rplL"
                     /locus_tag="ACHKG5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0868228.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHKG5_002124"
     CDS             4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHKG5_002124"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0868229.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHKG5_002125"
     CDS             8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHKG5_002125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0868230.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13136..13579
                     /locus_tag="ACHKG5_002126"
     CDS             13136..13579
                     /locus_tag="ACHKG5_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP0868231.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHKG5_002127"
     CDS             complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHKG5_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0868232.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHKG5_002128"
     CDS             complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHKG5_002128"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0868233.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHKG5_002129"
     CDS             complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHKG5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP0868234.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHKG5_002130"
     CDS             complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHKG5_002130"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0868235.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHKG5_002131"
     CDS             complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHKG5_002131"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0868236.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHKG5_002132"
     CDS             complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHKG5_002132"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0868237.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19459..19559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHKG5_002133"
     CDS             complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHKG5_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0868238.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20182..20955
                     /gene="nudC"
                     /locus_tag="ACHKG5_002134"
     CDS             20182..20955
                     /gene="nudC"
                     /locus_tag="ACHKG5_002134"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0868239.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20995..22059
                     /gene="hemE"
                     /locus_tag="ACHKG5_002135"
     CDS             20995..22059
                     /gene="hemE"
                     /locus_tag="ACHKG5_002135"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0868240.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22069..22740
                     /gene="nfi"
                     /locus_tag="ACHKG5_002136"
     CDS             22069..22740
                     /gene="nfi"
                     /locus_tag="ACHKG5_002136"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0868241.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHKG5_002137"
     CDS             22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHKG5_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0868242.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23560..23832
                     /gene="hupA"
                     /locus_tag="ACHKG5_002138"
     CDS             23560..23832
                     /gene="hupA"
                     /locus_tag="ACHKG5_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0868243.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHKG5_002139"
     CDS             23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHKG5_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0868244.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHKG5_002140"
     CDS             complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHKG5_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0868245.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25205..26581
                     /gene="zraS"
                     /locus_tag="ACHKG5_002141"
     CDS             25205..26581
                     /gene="zraS"
                     /locus_tag="ACHKG5_002141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0868246.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26578..27903
                     /gene="zraR"
                     /locus_tag="ACHKG5_002142"
     CDS             26578..27903
                     /gene="zraR"
                     /locus_tag="ACHKG5_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0868247.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHKG5_002143"
     CDS             complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHKG5_002143"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0868248.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHKG5_002144"
     CDS             complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHKG5_002144"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0868249.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7383 a         8112 c         8577 g         7290 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg caattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
      601 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
      661 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
      721 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
      781 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
      841 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
      901 ttgctcttgg tggcgattgt cggcaactat ctttatcgcg acattatgct gccgctgcgt
      961 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     1021 ggtaaagcta ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     1081 ccgactcgcc aggaaacatt gcacaccacg ctgatagtgg ctgcggttac cgcagtaatg
     1141 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     1201 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     1261 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     1321 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     1381 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     1441 cgacgcgagc tggcacctgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     1501 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     1561 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     1621 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     1681 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     1741 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     1801 cgcgagattg gaatacaatt tcgcgccttt tgtttttatg ggtcttgccc gtaaaacgat
     1861 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     1921 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     1981 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     2041 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     2101 tacgctgacc gttctttcac tttcgttacc aagactccgc cggcagcagt tctgctgaaa
     2161 aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     2221 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     2281 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     2341 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca
     2401 accaaacagt acgacatcaa cgaagctatc gctctgctga aagagctggc gactgctaaa
     2461 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     2521 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     2581 tttacccagg gtgcaaacgc tgaagctgct aaagctgcag gtgctgaact ggtaggtatg
     2641 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     2701 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg cggcctgatg
     2761 ccaaacccga aagtgggtac tgtaacgccg aacgttgctg aagcggttaa aaacgctaaa
     2821 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     2881 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     2941 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     3001 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     3061 tacgtgggcg gtgattttgt ctacaatctt acccccacgt atgctaacga aagttagcag
     3121 ctaaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag
     3181 tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta
     3241 tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag
     3301 tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt
     3361 caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca
     3421 gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt
     3481 cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa
     3541 ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct
     3601 atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa
     3661 tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc
     3721 ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa
     3781 gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct
     3841 taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga
     3901 acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta
     3961 atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct
     4021 gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta
     4081 attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact
     4141 ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa
     4201 ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa
     4261 gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg
     4321 tgacttttta gtcaccagcc tttttgcgct gtaaggcacc agtagcgttt cacactgttt
     4381 gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga
     4441 cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg
     4501 aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca
     4561 gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa
     4621 acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc
     4681 tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct
     4741 ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca
     4801 atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg
     4861 cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc
     4921 gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct
     4981 catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct
     5041 gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa
     5101 agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga
     5161 tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat
     5221 tctgcgtgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat
     5281 ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga
     5341 aaccgcatcc tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat
     5401 cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt
     5461 tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct
     5521 gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg
     5581 tcacaagcgt atcgaaacgc tgttcaccaa tgatctggat cacggcccgt atatctctga
     5641 aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat
     5701 gatgcgccct ggcgagccgc cgactcgtga agcagcggaa agcctgttcg agaacctgtt
     5761 cttctccgaa gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct
     5821 gctgcgcgaa gaaatcgaag gttctggtat cctgagcaaa gacgacatca ttgatgttat
     5881 gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg
     5941 caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt
     6001 acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc
     6061 tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag
     6121 ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg
     6181 tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga
     6241 cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat
     6301 cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac
     6361 tccgtatcgt aaagtgactg acggtgttgt aactgacgaa attcactacc tgtctgctat
     6421 cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt
     6481 cgtagaagac ctggtaacct gccgtagcaa aggcgaatcc agcttgttca gccgtgacca
     6541 ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc
     6601 gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc
     6661 cgttccgact ctgcgtgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc
     6721 cgttgactcc ggtgtaactg cggttgctaa acgtggtggt gtcgttcagt acgtggatgc
     6781 ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga
     6841 catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatta accagatgcc
     6901 gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac
     6961 cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg
     7021 ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac
     7081 caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga
     7141 gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg
     7201 tatcgtttat attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggttacgcc
     7261 gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa
     7321 agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat
     7381 cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga
     7441 agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc
     7501 gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct
     7561 cgacaaattg ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa
     7621 tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact
     7681 cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat
     7741 tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg
     7801 tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga
     7861 aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat
     7921 cggtcagatc ctcgaaaccc acttgggtat ggctgcgaaa ggtatcggcg acaagatcaa
     7981 cgccatgctg aaacagcagc aggaagtcgc gaaactgcgt gaattcatcc agcgtgcgta
     8041 cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt
     8101 tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg
     8161 tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga cttctggtca
     8221 gatccgcctg tacgacggcc gcactggtga acagttcgaa cgtccggtaa ccgttggtta
     8281 catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg
     8341 ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg
     8401 tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga
     8461 aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata aaaacatcgt
     8521 ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga
     8581 gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt
     8641 cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg
     8701 tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca
     8761 aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc
     8821 cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta
     8881 tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc
     8941 gtggcgtcat ctgtgagaag tgtggcgttg aagtgaccca gactaaagta cgccgtgagc
     9001 gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc
     9061 cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact
     9121 ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggagcgtcag cagatcctga
     9181 ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaaaatgg
     9241 gtgcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag tgcgaacagc
     9301 tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta
     9361 tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg
     9421 ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg
     9481 cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac
     9541 gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg
     9601 aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca
     9661 agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga
     9721 acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgtg ggtccatacc
     9781 tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca
     9841 tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg
     9901 ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg
     9961 tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac
    10021 tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact
    10081 tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc
    10141 gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg
    10201 ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca
    10261 aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc
    10321 tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg
    10381 gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga
    10441 tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa
    10501 tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg
    10561 cggaccagat catgtacacc ggctttgcat atgcagcgcg ttctggtgca tctgttggta
    10621 tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag
    10681 ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca
    10741 aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc
    10801 tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca
    10861 gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg
    10921 ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg
    10981 cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta
    11041 aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg
    11101 ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca
    11161 tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtattgg
    11221 gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca
    11281 acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta
    11341 aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc
    11401 gtgacctggc gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac
    11461 agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg
    11521 catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca
    11581 gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg
    11641 aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg
    11701 cggtactggc aaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg
    11761 acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga
    11821 tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg
    11881 ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg
    11941 ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc
    12001 tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc
    12061 tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccacgcg
    12121 ttgcggacct gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg
    12181 gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag
    12241 acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag
    12301 gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc
    12361 tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat
    12421 accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc
    12481 tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg
    12541 aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa
    12601 cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct
    12661 ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac
    12721 gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta
    12781 ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg
    12841 caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc
    12901 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
    12961 gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc
    13021 tgaatactca taatcaatgt agagatgact aatatcctga aactgactga actaattgag
    13081 tcaaactcgg caaggattcg atactattcc tgtgtagctt tcttaaggaa cgagaatgaa
    13141 acaggaagtg gagaagtggc gaccttttaa acatccggat ggcgacgttc gtgatttatc
    13201 gtttcttgat gctcatcagg ttatctacgt tcagcatcat gaggacaagg agcctttaga
    13261 gtatcgcttt tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac
    13321 gaacgaagaa aaactatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca
    13381 gcaccgttat aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag
    13441 caacgttatt catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg
    13501 agataaggca ttttactttg ttgtgttcag ggctttcagt gaaatttttc accattgcct
    13561 aaaacttatt gagaaataag cagcttcctc agccctcaaa ataacaaaac ccaccttaag
    13621 gtgggtttcg ccagagaatt atctctggta ttcagaacgc cattaccgga ctttgccttg
    13681 accttgcgat aatcgcaggt tgcgggatgt ctgaatttct tcagtctgct gcatcctgga
    13741 agatgagaac atgtgttctt attttcgtct ctatcatagt tgagtattta ctctcttaca
    13801 atcagatctc tttcattgct caacaggcga tggcttcaga ctttgcatta cggaattttt
    13861 aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt cctgctgacg
    13921 gcactaccat aaaaaaacac aacgctttca acaaccttgc gtttttcatc ttccgtagcg
    13981 gctcggaata aattttcgtt cttacattcc gtctcatagt ctttgcgagg cgcgtcccag
    14041 atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg cggcagcaac
    14101 cgcttccggt ctgcgctcgt cgtgcggtga gaactgttcc agctcggggt gattgtcggc
    14161 atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca gcggaataac
    14221 gcgatcgcga aaccacggtg attcccgcgt ggagagtgac agttcaatct ccggtgcaag
    14281 cagccggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg acgcaggctc
    14341 aatgccgcca gtacatggac gcaggcgtgg gaaggagaca gagtagcggc tttgccagta
    14401 atgctgttgc agccatagca aatgttctgc aaccatatag cagtcaacgc gccagctgtc
    14461 ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacggc ccagccgatc
    14521 cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcagatgat ggcgggcata
    14581 agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa gttgctttaa
    14641 ctcggcgtat tccgtctccg ccagcggctg tacttccatc tgtagtgaag agaactgttc
    14701 acgcagggca gggagatgac gacgaaagta atccatcccc actttcgcct gatgttcacc
    14761 ggtgactaaa agcagatgtt caaagcccat ctcccgtata gcggcgcttt ccctggcaat
    14821 atccgcttca tccagcgttt tgcgcttgat gcggttgctc atagagaatc cgcagtacgt
    14881 gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa ctgtgttgcc
    14941 aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat agccactggc
    15001 ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg
    15061 ctctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc agtccagttg
    15121 tcgccagcga tcgctgaagg ttttcatacc gatgcctcca gaaatccggt cagcgggctg
    15181 gtggcatgag caaaataact gcggctgccc ggtccggact gaggtgccag caggcctgct
    15241 tctactgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc
    15301 gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg gctgggaacg
    15361 ccgatgccag catcgacaac caccggaact gtggcctgct ggataataat ctccagcatg
    15421 gcgcgggttt ccagcccctg attcgagcca atcggcgcgc cgagcggcat caccgctgca
    15481 cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta aggcagcacg
    15541 acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg atcgggcaac
    15601 agccagcggg cgtcagggtg aatctccaat tttaaccagt ttgtgcctaa cgcttcacga
    15661 gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt atttggcagc
    15721 agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg
    15781 acacgtttca tcgccagcgt caccagctgg ctgccggaag cgcggattgc ctctaccatc
    15841 agttgtgaag aggcgaattt cccggtgccg gtaaacagat gtgaatcaaa cgttttgtcc
    15901 gcaatacgta acatctcaac cccctgcaat aacctgaaaa agtaggatct ggtcgccatc
    15961 ctgcacgata tgttgcgtcc actgctcacg cgggacgatt tgctgattaa tcgccagagc
    16021 cgcgcccgct tgtcgttgaa cgagttgctc cagtagttcg tgaacagttt gcccggcggc
    16081 gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat accgggcaac
    16141 cactggcgcg gcgcaacgcc aggctgcgcc actggctcga cttaccgtcg aacagtcgga
    16201 gttctcccgc aggcgtctct ataccgctta ataacttaat ggcttccagt gcctgcaaag
    16261 tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg cgttctggct
    16321 cctggttttc tggccacagg cagcggtaac aaccctgccc ccagggcggc gtcagtacca
    16381 tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg agtgccacgc
    16441 aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc accacatcgg
    16501 cttgtgcaac cgcatctttt aacgcctcac ccgttaatcg ttgttgtaat gctgtcagtt
    16561 gaatatcggg attcaactgt gtcagtcgct gatggctgac ctgcgatttc gggcgatcga
    16621 tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat
    16681 ctgccagtac cagcgtcccg acgccagcgc ccgccaggta tagcgcagca ggtgtaccca
    16741 gcccgccaag accgataatc agcacctggc tgtcgagcag tttttgctgc ccgtcaagag
    16801 cgatatcgtc gagcaggatt tggcggctat aacgcataaa atcacggtca ttcatcgcca
    16861 actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg
    16921 gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga
    16981 ctgataccgc caatcgccac ggtgggataa tccgacagtc gctcaacatg ccgtgccagc
    17041 tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc
    17101 agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc gtcatgggtc
    17161 gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc ttgcaaatct
    17221 tcctgcccca aatggacgcc atacgcctga tgcttgatcg cgagccgcca gtaatcgttg
    17281 ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac atcggcttcc
    17341 acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc tgcatccaac
    17401 agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg aaaaggtaca
    17461 ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt tctccgcctc
    17521 tggcacggaa gttctccgac atatccgcta ttcccatttc aatagtttgc gtggcggcgt
    17581 aatcacgcac ttcctggctg attttcatcg agcagaattt cggcccacac atggagcaaa
    17641 aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg cgggcggtaa
    17701 acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca
    17761 tggcgttatc gcgaatttgt gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg
    17821 caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga cccagatgct
    17881 cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc gccgcaccaa
    17941 tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc cccagagtgt
    18001 aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg atcatctgca
    18061 tcggcacgtg gcctgggcct tcaatcatca cctgcacatc atattcccag gcgatttttg
    18121 tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg tcctgaatag
    18181 aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca cagatttcac
    18241 aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg caccatttcg
    18301 ccataatcga accgccgcgc gagacgatac cggtcaggcg tttcgcggtc atcggcacat
    18361 agcgcagcag cacccccgca tggatagtga agtaatccac accttgctcg gcctgttcca
    18421 gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccatta accttctcca
    18481 gcgcctggta gatcggcact gtaccaatcg gcaccgggct gttacgcaaa atccactcgc
    18541 gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct ccccagcgcg
    18601 tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc gagttgccga
    18661 tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc gattccggat
    18721 gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg acaaattccg
    18781 cagtgatatt ttccggcaga cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa
    18841 cttcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat tccatttccg
    18901 gtgtgatgat gccctggcgg gcgtagtgca gttgtgtgac gcggcgtcct gctttggcgc
    18961 gttttggcgt taatacgccg ctaaaacgca gctcgtcgag gccatcatct gccagccgcg
    19021 ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc
    19081 acggctggcg tagttttgcc agcccttgct gcacgttaat ggcgatctgc ggatcgccat
    19141 acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc ggctgttctt
    19201 tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg cgcacgccgg
    19261 gttgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct tccagggtgt
    19321 cgataaaatg ttgggcctgg gcgcgttgtt cgcgacgggt cagttttgtt gcagacatag
    19381 ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa gagacgatcg
    19441 ccgcaaaggc gatgcgatag cagattaact cttgttccct tcgcaggtat tagcctgatc
    19501 aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga caagaaatta
    19561 gccccacaaa cggggcgttg aatgtaaatt acgcgttaac agcgcagaac tcaagcagga
    19621 tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag cacaaagcgc
    19681 gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta attatcatga
    19741 tcgatagcgg tttccaggct ggaatcgtag taatccataa tctgttgggt attggcttcg
    19801 agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga
    19861 atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct ctgacaaaaa
    19921 tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt aatgccaacc
    19981 agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc aaccagtttg
    20041 ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat tgactccgca
    20101 agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac atgccagtga
    20161 tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca cggctggtgg
    20221 gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata tggcgaagcg
    20281 gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca gggggaacct
    20341 gtttggttaa tacaacagca gcggcgttac gatatggggt cggtacgtca ggtcattgat
    20401 ctcgatgttg ggctgtttca actggccgga cgtggcgtac aactggcgga gttttaccga
    20461 tcgcataaat actgtggtta ctgcgggcat gaaatgtatc caagcaaaac cgaatgggcg
    20521 atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg cattattgtt
    20581 gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg taacggtgtc
    20641 catacggtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg
    20701 gaagtgatgg aagagagcgg aattaaagtt aaacacttgc gttatgtgac ttcccagccg
    20761 tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag cggcgacatc
    20821 gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga tttgccgtta
    20881 ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg
    20941 gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc caaaatgacc
    21001 gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga tgtcactcct
    21061 gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac gcgcgcccag
    21121 gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgtgaagt gaccttgcaa
    21181 ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct caccgtcccg
    21241 gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt tacctcgcca
    21301 gtcacctgca aagctgacgt cgataaactg ccaattccgg acccggaaga tgaactgggg
    21361 tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt gccgctgatt
    21421 ggtttttccg gcagcccgtg gacgctggcg acctacatgg tggaaggcgg cagcagcaaa
    21481 gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca cgctctactc
    21541 gataaactgg cgaaaagcgt cgctttgtat ctgaatgcgc agattaaagc cggtgctcag
    21601 gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc
    21661 tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga cggtcgccgc
    21721 gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat ggcagaaacc
    21781 ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg
    21841 ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc
    21901 cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga aggtcatgtc
    21961 tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg
    22021 gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagcgattat ggatctcgcg
    22081 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga tcgactcgat
    22141 aaggatccgc cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    22201 acgcgagcgg cgatggtgct gctgaaatac ccctcgcttg agctggtgga gtataaagtg
    22261 gcccgcatcg ctaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    22321 gcgctgctgg cagcgtggga gatgctgtca caaaagccgg atttagtgtt tgtcgatggt
    22381 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggttt gctggtggat
    22441 gtgccgacca ttggcgtagc gaaaaaacga ctatgcggta aattcgaacc gctctccagc
    22501 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    22561 agcaaagcgc gctgtaaccc gctatttatc gctactggtc atcgggtcag cgtggacagc
    22621 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    22681 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22741 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22801 catctgcgtc tggaacgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22861 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22921 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga tgcaaaagta
    22981 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    23041 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    23101 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    23161 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gctcaaagaa
    23221 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    23281 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgaggcggat
    23341 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    23401 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    23461 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    23521 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    23581 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    23641 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    23701 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23761 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23821 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23881 ttgtctttct ggcgtcgatc attgatgctg gctggcgctc tgtttctcac tgcttgtagt
    23941 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    24001 cgcatctggc gaaaagacag cggcgataat gtgcatcttc ttgccgtgtt tagcccgtgg
    24061 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    24121 aatatcaatg tttacagcaa acccccggtg aatattcgcg cgcgttttga cgatcgcggt
    24181 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    24241 atcgatttat accgttatcg ggctgatcag atccgccaga ttagcgatgc cttacgtcag
    24301 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    24361 gaagggcaaa ccattaaacc tgatttagat tcgcaggcaa tagcgcatat cgagcgccgc
    24421 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    24481 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    24541 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    24601 ttccggctcc gcgcggaata cccgcttcag ccatcgcaat atcccgtttc acccgtaact
    24661 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24721 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24781 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24841 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24901 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24961 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    25021 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    25081 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    25141 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    25201 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    25261 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    25321 ccgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgtgc
    25381 tctcgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    25441 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    25501 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attccccgca
    25561 ggaaatgcag cagttacatc cgggagatga agaagtgtgg cggcggatcg atagcgcaga
    25621 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    25681 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25741 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttatcctctt
    25801 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25861 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25921 gcatcttgcg gcaggcgttg cccacgaaat tcgtaacccg ctttcctcga ttaaagggct
    25981 ggcaaaatac tttgccgagc gcgcgccagc agggggagaa gcgcatcaac tggcgcaggt
    26041 gatggcgaaa gaagctgacc gtttaaaccg cgtggtaagc gagctgctgg aactggttaa
    26101 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    26161 gctggtaagc caggatgcaa actgccggga gatccagtta cgctttaccg ccaacgacac
    26221 gttgccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctttatct
    26281 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    26341 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    26401 agccatcttc accccgtact tcaccaccaa agccgaaggc accggactgg ggctggcggt
    26461 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc tggagggaaa
    26521 aggcgcaagg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    26581 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    26641 caggctttgc tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    26701 ctggagcagg tgcgggagcg ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26761 atggacggca ttgccacgct aaaagagatc aaagcgttaa atccggcgat tccggtactg
    26821 attatgaccg catactctag cgtcgagacg gcggtagagg cgttgaaaac cggggcgctg
    26881 gattatctca tcaagccgct ggatttcgat aacctgcagg cgacgctgga aaaggcgctc
    26941 gcgcatacgc acattattga tgctgaaacg cctgcggtga ctgccagcca gttcggtatg
    27001 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    27061 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    27121 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    27181 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    27241 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgttgtt tctcgatgaa
    27301 attggcgata tctcgccgat gatgctggtg cgtctgctgc gtgcgattca ggagcgcgaa
    27361 gttcagcgtg tcggtagcaa ccagactatt tcggttgatg tccggctgat tgccgcgacc
    27421 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    27481 ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tattcctctg
    27541 ctggctggcc attttctgca gcgctttgct gaacgtaatc gcaaggcggt aaaaggcttt
    27601 acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat tcgtgaactg
    27661 gaaaatgcgg tggaacgggc ggtggtgctg ctgactgggg aatatatttc cgaacgcgag
    27721 ctgccgctgg cgattgccag tacgccgatc ccgctggcac aaagcctgga tattcagccg
    27781 ctggtggaag tcgaaaaaga agtgattctg gcggcactgg agaaaacggg cggcaacaaa
    27841 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tgttggcaaa attgtcgcgt
    27901 tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc
    27961 cagtggatat cggtcattaa ggcataggcg cgtttctgtg cttctgctac ggtatgaccc
    28021 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    28081 tttgtgcccg cgtggaacac tttgccgtct tccacttctt ccagcggcag gccgtggatc
    28141 acgtcaccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gccgagagaa
    28201 gcacgttcat cccactctga cgttttctcg tctagtttgc cttcacaggc cgccaggcag
    28261 agttcaacaa gatcggactt catgcgcagc ataatcggct gggtttctgg atcgccaaag
    28321 cggcagttaa attcgataac cttcggattg ccctgtttgt cgatcatcag gcccgcgtag
    28381 agaaaaccgg tgtaggtgtt gccttccgac gccatgcctt tcacggttgg ccagatgata
    28441 cgttccatcg tgcgctgatg aacgtcatct gtcactaccg gcgcaggaga gtaagcgccc
    28501 attccgcccg tgttcggccc ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg
    28561 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccgtcg
    28621 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa acgcgttgcc cgccagcata
    28681 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28741 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata cgccagcgca
    28801 ggttctacct cggtgaagtt ctgatattcc gccgtaggaa tgttatggcg ggccaggaaa
    28861 tctttagtaa acgctttcga gccttccagc tgtgcggcgc ctgcggttgg gccgaagatt
    28921 ttcatcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28981 acgatggtta gatctacctt ttcgttttgc gcgaaatcca gcagcgccgg gatatcggtc
    29041 acgccgatag cgacgttttg cagcgtgggt tccagcgctg tgcctgcgtt acccggagca
    29101 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    29161 ccgccgttac caatcactaa tactttcatc tgtcgctcca ttaatggcgg aagtggcgca
    29221 tatcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    29281 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    29341 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    29401 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    29461 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaacttcg
    29521 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgtttggtga
    29581 cgacgcgcag ttcttccgca ccgaccatgc ccagatcgcg atcctgaacc agcagaccgc
    29641 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    29701 cgcgtacatt ctgtttggcg gcggtgattt tcagggcttc ttcgctggcg gacggcgcaa
    29761 taatcacttc aacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    29821 ggttaaaggc aatgatgccg ccgaatgcgg aggtcgggtc ggttttgtac gcgcgatcgt
    29881 aagcatcaag aattgaattg ccgatagcca cgccgcaagg gttggcgtgc ttcacaatca
    29941 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    30001 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    30061 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    30121 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    30181 caccgtgata agccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    30241 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    30301 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    30361 catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata tcgatgttct
    30421 caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac ggatacaggt
    30481 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    30541 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    30601 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    30661 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    30721 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30781 gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc tgaatttttc
    30841 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30901 aaaacgtttg cgcaacgctc gcgaattttt ttaattcgat ggcgatcacg attttgcccc
    30961 cgattagcgt gggagaaata gagaaataac gctgatgatt gtggataact ctgtgcgtaa
    31021 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    31081 tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg cggatgtgaa
    31141 tcacttcaca caaacagccg gttcggttga agagaaaaat tctgaaattc agggttgact
    31201 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag ccgcgtcgca
    31261 actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa
    31321 cgtcgcaaga cgaaaaatga ataccaagtc tcaagagtga ac
//
LOCUS       DAZGXS010000044        30907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGXS010000044 DAZGXS010000000
VERSION     DAZGXS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(33..3107)
                     /gene="lacZ"
                     /locus_tag="ACHKG5_002145"
     CDS             complement(33..3107)
                     /gene="lacZ"
                     /locus_tag="ACHKG5_002145"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0868250.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(3230..4312)
                     /gene="lacI"
                     /locus_tag="ACHKG5_002146"
     CDS             complement(3230..4312)
                     /gene="lacI"
                     /locus_tag="ACHKG5_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0868251.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(4349..5302)
                     /locus_tag="ACHKG5_002147"
     CDS             complement(4349..5302)
                     /locus_tag="ACHKG5_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFP0868252.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(5322..6098)
                     /locus_tag="ACHKG5_002148"
     CDS             complement(5322..6098)
                     /locus_tag="ACHKG5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP0868253.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(6213..7046)
                     /gene="mhpR"
                     /locus_tag="ACHKG5_002149"
     CDS             complement(6213..7046)
                     /gene="mhpR"
                     /locus_tag="ACHKG5_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0868254.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7237..8901
                     /gene="mhpA"
                     /locus_tag="ACHKG5_002150"
     CDS             7237..8901
                     /gene="mhpA"
                     /locus_tag="ACHKG5_002150"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0868255.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            8903..9847
                     /gene="mhpB"
                     /locus_tag="ACHKG5_002151"
     CDS             8903..9847
                     /gene="mhpB"
                     /locus_tag="ACHKG5_002151"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0868256.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            9865..10731
                     /gene="mhpC"
                     /locus_tag="ACHKG5_002152"
     CDS             9865..10731
                     /gene="mhpC"
                     /locus_tag="ACHKG5_002152"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0868257.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            10741..11550
                     /gene="mhpD"
                     /locus_tag="ACHKG5_002153"
     CDS             10741..11550
                     /gene="mhpD"
                     /locus_tag="ACHKG5_002153"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0868258.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            11547..12497
                     /gene="mhpF"
                     /locus_tag="ACHKG5_002154"
     CDS             11547..12497
                     /gene="mhpF"
                     /locus_tag="ACHKG5_002154"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0868259.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKIAQSLARKAGEAA"
     gene            12494..13507
                     /gene="mhpE"
                     /locus_tag="ACHKG5_002155"
     CDS             12494..13507
                     /gene="mhpE"
                     /locus_tag="ACHKG5_002155"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP0868260.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            13683..14894
                     /gene="mhpT"
                     /locus_tag="ACHKG5_002156"
     CDS             13683..14894
                     /gene="mhpT"
                     /locus_tag="ACHKG5_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0868261.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            14996..15535
                     /gene="yaiL"
                     /locus_tag="ACHKG5_002157"
     CDS             14996..15535
                     /gene="yaiL"
                     /locus_tag="ACHKG5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0868262.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(15660..16493)
                     /gene="fghA"
                     /locus_tag="ACHKG5_002158"
     CDS             complement(15660..16493)
                     /gene="fghA"
                     /locus_tag="ACHKG5_002158"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0868263.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(16586..17695)
                     /gene="frmA"
                     /locus_tag="ACHKG5_002159"
     CDS             complement(16586..17695)
                     /gene="frmA"
                     /locus_tag="ACHKG5_002159"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0868264.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(17730..18005)
                     /gene="frmR"
                     /locus_tag="ACHKG5_002160"
     CDS             complement(17730..18005)
                     /gene="frmR"
                     /locus_tag="ACHKG5_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0868265.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(18165..19211)
                     /gene="fbpC"
                     /locus_tag="ACHKG5_002161"
     CDS             complement(18165..19211)
                     /gene="fbpC"
                     /locus_tag="ACHKG5_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP0868266.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(19223..21301)
                     /locus_tag="ACHKG5_002162"
     CDS             complement(19223..21301)
                     /locus_tag="ACHKG5_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868267.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(21370..22401)
                     /locus_tag="ACHKG5_002163"
     CDS             complement(21370..22401)
                     /locus_tag="ACHKG5_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0868268.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(22398..23714)
                     /gene="uhpC"
                     /locus_tag="ACHKG5_002164"
     CDS             complement(22398..23714)
                     /gene="uhpC"
                     /locus_tag="ACHKG5_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0868269.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(23787..25328)
                     /locus_tag="ACHKG5_002165"
     CDS             complement(23787..25328)
                     /locus_tag="ACHKG5_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0868270.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPTQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(25328..25957)
                     /locus_tag="ACHKG5_002166"
     CDS             complement(25328..25957)
                     /locus_tag="ACHKG5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP0868271.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            26260..27222
                     /gene="tauA"
                     /locus_tag="ACHKG5_002167"
     CDS             26260..27222
                     /gene="tauA"
                     /locus_tag="ACHKG5_002167"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0868272.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            27235..28002
                     /gene="tauB"
                     /locus_tag="ACHKG5_002168"
     CDS             27235..28002
                     /gene="tauB"
                     /locus_tag="ACHKG5_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP0868273.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            27999..28826
                     /gene="tauC"
                     /locus_tag="ACHKG5_002169"
     CDS             27999..28826
                     /gene="tauC"
                     /locus_tag="ACHKG5_002169"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP0868274.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            28823..29674
                     /gene="tauD"
                     /locus_tag="ACHKG5_002170"
     CDS             28823..29674
                     /gene="tauD"
                     /locus_tag="ACHKG5_002170"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0868275.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(29781..30755)
                     /gene="hemB"
                     /locus_tag="ACHKG5_002171"
     CDS             complement(29781..30755)
                     /gene="hemB"
                     /locus_tag="ACHKG5_002171"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0868276.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         7101 a         8213 c         8561 g         7032 t
ORIGIN      
        1 aatacgggca gacatggcct gcccggttat tattattttt gacaccagac caactggtaa
       61 tggtagcgac cggcgctcag ctggaattcc gccgatactg acgggctcca ggagtcgtcg
      121 ccaccaatcc ccatatggaa accgtcgata ttcagccatg ttccttcttc cgcgtgcagc
      181 agatggcgat ggctggtttc catcagttgt tgttggctgt agcggctgat gttgaactgg
      241 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg
      301 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca
      361 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt
      421 acccgctctg ctacctgcgc cagctggcag gtcaggccaa tccgtgccgg atgcggtgta
      481 tcgctcgcta cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag
      541 gttttccggc taataaataa ggtttttccc tgatgctgcc atgcgtggac agtggtaatc
      601 agcaccgcgt cggcaagtgt atctgccgtg cactgcaaca acgccgcttc tgcctggtaa
      661 tggcccgcag ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt
      721 acgccaatgt cgttatccag cggtgcgcgg gtgaactgat cgcgcagcgg cgtcagcagt
      781 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa
      841 cgcttattat ccagctcgat gcaaaaatcc gtttcgctgg tggtcagttg cgggatggcg
      901 tggggcgcgg aggggagtgt cacgctgagg ttttccgcca gacgccactg ctgccaggcg
      961 ctgatgtgtc cggcttctga ccatgcggtc gcgttcggtt gcactacgtg taccgttagc
     1021 cagagttgcc cggtgctctc cagtcgcggt agttcaggca attcaattac ctgtttacct
     1081 tgcggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc
     1141 gtccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg
     1201 gttcgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgcc
     1261 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatgc agaactggcg atcattcggc
     1321 gtatcgccaa aatcaccgcc gtaagccgac cacggattgc cattttcatc atatttaatc
     1381 agcgactgat ccaccaagtc ccagacgaag ccgccctgta aacgggggta ctgacgaaac
     1441 gcctgccagt atttagcgaa gccgccaaga ctgttaccca tcgcgtgggc gtattcgcaa
     1501 aggatcagtg ggcgcatttc tccaggcagc gaaagccatt ttttgatgga ccatttcggc
     1561 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtt
     1621 gcggaggtgt cggctccgcc gccttcatac tgcaccgggc gggatggatc gacagattta
     1681 atccagcgat agagtgcgtc gtgattagcg ccgtggcctg actcattccc cagcgaccag
     1741 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gtgttactcg ttcgctcatg
     1801 gccggtaacc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca
     1861 atattggctt catccaccac atacaggccg tagcggtcgc acagggtgta ccacagcgga
     1921 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat tagcaggata
     1981 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg
     2041 ttaacgccgc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc
     2101 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc
     2161 agttcaacca ccgcacgata gatattcggg atttcggcgc tccacagttt cgggttttcg
     2221 acgttcagac ctagtgtgac gcgatcggca taaccgccac gctcatcgat aatttcaccg
     2281 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccacaaaga aaccgtcacc
     2341 cgcagctcat cgcgcagctc gccgtacatc tgaacttctg cctccagtac cgcgcggcta
     2401 aaatcatcat taaagagagt ggcaacatgg aaatcgctga tttgtgtggt cggtttgtgc
     2461 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa
     2521 ctgccgtcac tccagcgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat
     2581 gcgctcaggt caaattcaga cagcaaacga ctgtcctggc cgtaaccgac ccagcgcccg
     2641 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc
     2701 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg
     2761 ggaacaaatg gcggattgac cgtaatggga tatgtcacgt tggtgtagat gggcgcgtcg
     2821 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcgtcagg aagatcgcac
     2881 tccagccagc tttccggaac cgcttctggt gccggaaacc agacaaattg ccactcacca
     2941 ttcaggctgc gcaactgctg ggaagggcga ttggtgcggg cctcttcgct attacgccag
     3001 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3061 tcacgacgtt gtaatacgac ggccagtgaa tctgtaatca tagtcatagc tgtatcctgt
     3121 gtgaaattgt tatccgctca caatttcaca caacatacga gccggacgca tagagtgtaa
     3181 agcgtggggt gcctaatgag tgagctgact cacattaatt gcgttgcgct cactgcccgc
     3241 tttccagtcg ggaaacttgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3301 aagcggtttg cgtcttgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
     3361 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     3421 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa cacgagctgt
     3481 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcaac ccggactcgg
     3541 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     3601 cgatgccctc atttagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     3661 cctcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagcccgcca
     3721 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     3781 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     3841 tgttgatggg agtctggtca gagacatcaa gaaataacgc cggaacatta gcgcaggcag
     3901 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     3961 gttgcgcgag aagattgtgc actgccgctt tacaggcttc gacgccgctt cgttctacca
     4021 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     4081 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttcc
     4141 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
     4201 ctttttcccg cgttttcgca gaaacgtggc tggcctgatt caccacgcgg gaaacggtct
     4261 gataagagac accggcatac tctgcgacat cgtatagcgt tactggtttc acattcacca
     4321 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
     4381 cggcggggct gacgccgaag tgagatttaa aatgctgacg ccaggtcaca ggtgagagaa
     4441 aacccacctc agccgctatc cgctcaacgg gcaactgact ggatcccaac agttcctggc
     4501 tacggcgtaa gcgttcagta atgagccatt cggcgatact cgaaccggtc gctttcataa
     4561 aatggcgagt taatgtgcgg cgactcatca ttactcgctg cgccagcgag tcgagatcat
     4621 gctgttgcgc aatgttttgc cgcaggtaat ccagcaggag gtttatgcgt tcatcatggg
     4681 tatttttcgg caccggttgc tcaataaact gcgcctgacc gccggtgcga tatggcggta
     4741 cgaccatccg tcgggcaata tggttagcat agtcgctgcc aaaatgttgc cgaacaatat
     4801 acaaacagca atccagcgcc gcggcagtac cggcagaggt aataatgccg tcatcgtcta
     4861 cgtacaacgc gttaatatcc agatgtgttt gtggaaaacg tgcctgaaat tcacgctcga
     4921 actcccagtg cgtggcggca cgcttatttt tcagtaaacc tgcatagccg agcacaaacg
     4981 tgcccaggca aagcccgaca atctgtgcgc cgttatcccg tgcggtcgtt aaggcttcca
     5041 gcagtttttg tggcggtttt tgtgtaatgg ttccccagta aggaattatc acaatatctg
     5101 cctggatgac tgcggtatag tcatgggtgg cggttacgga aaacccatcg gcagagtcca
     5161 cgtttcccgg cttttcagcg caaataatca cgtgaaagcg ttttttctcc gccatctttt
     5221 cactaaacac gataaacggt acggaaaggt gaaaaggact aaaaccggca acggcgataa
     5281 tggcaacagt tagcgggcgc ataacactct ttatctccga attagaatgt caacgtttca
     5341 ccatcttctg gtacgttcac aaattcgata atttgattat ctctggcgta ttccttgagc
     5401 gcatcacgcg tcagcaaaca gtggtttacg gcctccatat gtgtcgcgat aatttgcgct
     5461 tctgggaggg taaaatgtgt tttgagaata tcttctgctc ccataattat cgcgccatgc
     5521 cccagaacat gcgcaaatcc ggcattcaac acaaccactt ctggttggaa agttagcatt
     5581 gtagcctcga cgtcgtcacg ccagagggta tcgccagcca gatagagcgt tttctctgtc
     5641 gggtgctgga aaacaacgcc gcaagctgcg cctaaacgct tcgccatcgc cgggtcggca
     5701 taggtgcgat cggagccgtg ctgccctgtc gttttatgca gagtaatatc gccaaattta
     5761 gtctcgtcgg taagtacggt aagatgcgta aatccttgtt tattcagtga ttcagcatct
     5821 tgctcatttt gcacataaat cagtttatct ttagggacga cgcgggaggc tgtttcgtcc
     5881 cagtgatctt catgcaaatg ggtcacgatt agggcgtcaa cgttgagtaa cgttgttaaa
     5941 tcgcacggta attctaccgt tggattgcgg atttctgcgc gtgccgttcc ggcaaaaccc
     6001 ggataacttc ctttaggcgc gagtaacggg tcgataagaa atgttttgcc agcgtattgg
     6061 ataatttggg tggcattacg aatttgcgta attttcatga tttgctccac atcacagtat
     6121 tgatgtcatt agcgtagcgt gcagcccgta ctgtgcatgt gggcaaatgg gcgaataacg
     6181 atgagaatgg gccaaattac acattggcgt aactagcgta aatgcacgcc gcttcgccgt
     6241 ccggccacca gaatagcctg cgattcaacc ccttcttcga tctgttttgc tacccgttgt
     6301 agtgccggaa gatgcttttc cgctgcctgt tcgatggtca ttgcgctcgc catatacacc
     6361 agattcagac agccaatcac ccgttgttca ctgcgcagcg gtacggcgat agaggcgatc
     6421 ttctcctcct gatcccagcc gcggtagttc tgtccgtaac cctctttgcg cgcgcgcgcc
     6481 agaatggctt ccagctttaa cggttctcgt gccagttgat agtcatcacc ggggcgggag
     6541 gctaacatct cgattaattc cttgcgttct tgttccgggc aaaaggccag ccaggtcagg
     6601 cccgaggcgg ttttcagaag cggtaaacgc cgcccgacca ttgcccggtg aaaggataag
     6661 cggctgaaac ggtgagtggt ttcgcgtacc accattgcat caacatccag cgtggacaca
     6721 tctgtcggcc ataccacttc gcgcaacaga tcgcccagca gtggggccgc cagtgcagaa
     6781 atccactgtt cgtcacgaaa tccttcgctt aattgccgca ctttgatggt cagacgaaaa
     6841 ctatcatcgg agggactacg ccggacatat ccctcttcct gcagcgtctc cagcagtcgc
     6901 cgcacagtgg tgcgatgcag gccgctgagt tccgccagca gcccgacgct ggcaccgccg
     6961 tcaagtttat ttaacatatt taataacatt agaccgcggg ttaagccgcg cacggttttg
     7021 tattccgtct gctcattgtt ctgcatatta attgacattt ctatagttaa aacaacgttg
     7081 tgcacctggt gcacattcgg gcatgttttg attgtagccg aaaacaccct ttctatactg
     7141 agcgcacaat aaaaaatcat ttacatattt ttaacaaaat aagttgcgct gtactgtgcg
     7201 cgcaacggca ttttgtccga gtcgtgaggt actgaaatgg caatacaaca ccctgacatc
     7261 cagcctgctg ttaaccatag cgttcaggtg gcgatcgctg gtgccggtcc ggtcgggctg
     7321 atgatggcga actatctcgg ccagatgggc attgacgtgc tggtggtgga gaaactcgat
     7381 aagttgatcg actacccgcg tgcgattggt attgatgacg aggcgctgcg caccatgcag
     7441 tcggtcggcc tggtcgataa tgttctgccg catactacgc cgtggcacgc gatgcgtttt
     7501 ctcaccccga agggccgctg ttttgctgat attcagccaa tgaccgatga atttggctgg
     7561 ccgcgccgta acgcctttat tcagccgcag gtcgatgcgg tgatgctgga aggggtgtcg
     7621 cgttttccga atgtgcgctg cttgttttcc cgcgagctgg aggccttcag tcagcaagat
     7681 gacgaagtga ccttgcacct gaaaacggca gaagggctgc gggaaatagt caaagcccag
     7741 tggctggtgg cctgtgatgg tggggcaagt tttgtccgtc gcaccctgaa tgtgccgttt
     7801 gaaggtaaaa ctgcgccaaa tcagtggatt gtggtagata tcgccaacga tccgttaagt
     7861 acgccgcata tctatttgtg ttgcgatccg gtgcgcccgt atgtttctgc cgcgctacct
     7921 catgcggtac gtcgctttga atttatggtg atgccgggag aaaccgaaga acagctgcgt
     7981 gagccgcaaa atatgcgcaa gctgttaagc aaagtgctgc ctaatccgga caatgttgaa
     8041 ttgattcgcc agcgtgtcta cacccacaac gcgcgactgg cgcaacgttt ccgtattgat
     8101 cgcgtactgc tggcgggcga tgccgcgcac atcatgccgg tatggcaggg gcagggctat
     8161 aacagtggta tgcgcgacgc ctttaacctc gcatggaaac tggcgttggt tatccagggg
     8221 aaagcccgcg atgcgctgct cgatacctat caacaagaac gtcgcgatca cgccaaagcg
     8281 atgattgacc tgtccgtgac ggcgggcaac gtgctggctc cgccgaaacg ctggcagggt
     8341 acgttacgtg acggcgtttc ctggctgctg aattatctgc cgccagtaaa acgctacttc
     8401 ctcgaaatgc gcttcaagcc gatgccgcaa tattacggcg gtgcgctggt gcgtgagggc
     8461 gaagcgaagc actctccggt cggcaagatg tttattcagc cgaaagtcac gctggaaaac
     8521 ggcgacgtga cgctgctcga taacgcgatc ggcgcgaact tcgcggtaat tggctgggga
     8581 tgcaatccac tgtgggggat gagcgacgag caaatccagc agtggcgcgc gttgagcaca
     8641 cgcttcattc aggtggtgcc ggaagtgcaa attcataccg cacaggataa ccacgacggc
     8701 gtactacgcg tgggggatac gcaaggtcgc ctgcgtagct ggttcgcgca acacaatgct
     8761 tcgctggtgg tgatgcgccc ggatcgcttt gttgccgcca ccgccattcc gcaaacactg
     8821 ggcaagaccc tgaataaact ggcgtcggtg atgacgctga cccgccctga tgccgacgtt
     8881 tctgtcgaaa aggtagcctg atatgcacgc ttatcttcat tgtctttccc actcgccgct
     8941 ggtggggtat gtcgacccgg cacaagaggt actcgatgag gtcaatggcg tgattgccag
     9001 cgcccgcgag cgtattgcgg ctttctcccc tgaactggtg gtgctgtttg cgccagatca
     9061 ctacaacggc tttttctatg acgtgatgcc accgttctgt ttaggcgttg gagcgacggc
     9121 aattggtgat ttcggcagtg cggcaggaga gctgcccgtg cctgtggagc tggcggaggc
     9181 ctgtgcgcat gccgtcatga agagcgggat cgatcttgcc gtttcttact gtatgcaggt
     9241 ggaccacggg ttcgcccagc cgctggagtt cctgctcggt gggctggata aggtgccagt
     9301 tctgcctgtg ttcatcaacg gtgtcgccac gccgctgccc ggtttccagc gtacccgcat
     9361 gttgggtgaa gccattggac gtttcaccag cactctcaat aaacgcgtgc tgttcctagg
     9421 ttccggtggg ctttcccatc agccgccggt gcccgaactg gcgaaagccg atgcccatat
     9481 gcgcgaccgc ctgttgggga gcgggaaaga tttacccgcc agtgagcgcg aattgcgtca
     9541 gcaacgggtg attagcgccg ctgagaagtt tgttgaggat cagagaactc tgcatccgct
     9601 caacccgatt tgggataacc agttcatgac tttgctggag cagggacgca tacaggaact
     9661 ggatgccgtc agtaacgaag agctttccgc cattgccgga aagtcgacac atgaaatcaa
     9721 aacctgggtc gccgcttttg ccgctatttc tgcatttggc aactggcgta gcgaagggcg
     9781 ttattaccgc ccaatcccgg agtggattgc cggatttggc tcgttaagcg ccagaacaga
     9841 gaactgaata tgcaggagaa gatgatgagt tatcagccac aaaccgaagc cgccaccagc
     9901 cgttttctga atgtagaaga agcgggtaaa acgctgcgca tccattttaa tgactgcgga
     9961 caaggcgacg aaaccgttgt cctgctgcat ggttccggcc cgggtgctac tggctgggcg
    10021 aacttcagcc gcaatatcga tccgctggta gaggcgggct atcgggtgat cctgctggat
    10081 tgtccgggtt ggggcaagag cgattcgatc gttaatagtg gttcgcgatc ggatcttaat
    10141 gcacgaatcc tgaaaagcgt ggtggatcaa ctggatatcg ccaaaatcca cctgctgggc
    10201 aactcgatgg gtggccatag ttctgtggcg ttcaccctta actggccgga gcgcgtcggc
    10261 aaactggtgc tgatgggcgg cggtacgggc ggcatgagtt tgtttacgcc gatgccaacc
    10321 gaaggtatta agcgactgaa tcagctttat cgtcagccga ctatcgaaaa cctgaagctg
    10381 atgatggata tcttcgtttt tgataccagc gatttgaccg acgccctgtt tgaagcgcgc
    10441 ctgaataata tgctgtcgcg ccgcgatcac ctggaaaact tcgttaagag cctggaagct
    10501 aatccgaaac agttcccgga ttttggccca cgtctggcgg aaatcaaagc gcaaaccctg
    10561 attgtctggg ggcgcaacga ccgctttgtg ccgatggatg cgggtctgcg tctgctgtcc
    10621 ggcattgccg gttctgaact gcatatcttc cgcgactgtg ggcattgggc gcagtgggaa
    10681 catgccgacg ctttcaatca actggtgctg aatttcctcg cacgtgctta aggaacggtc
    10741 atgacgaagc atactcttga gcaactggcg gcggatttac gccgcgccgc agagcagggc
    10801 gaagcgattg cgccgctgcg cgatctgatt ggtatcgata acgctgaagc ggcctacgcc
    10861 attcagcaca taaatgtgca atatgacgtt gcgcaggggc gtcgcgtggt ggggcgtaaa
    10921 gtgggcctga cacatccgaa agtgcagcaa caactgggcg ttgatcaacc ggatttcggg
    10981 acattatttg ccgacatgtg ttatggcgat aacgaaatca ttcctttttc ccgtgttctg
    11041 caaccccgca ttgaagcgga gatcgcactg gtgttgaacc gcgatttacc cgcaactgat
    11101 atcaccttcg acgaattgta taacgccatt gaatgggtac ttccggcgct ggaggtggtg
    11161 ggtagccgca ttcgcgactg gtcgattcag tttgtcgata ccgtggcaga taacgcttcc
    11221 tgcggggtgt atgtcatcgg cggtccggcg caacgtccgg cggggttaga cctgaaaaac
    11281 tgcgccatga agatgacgcg taataacgaa gaggtttcca gcgggcgcgg cagcgaatgc
    11341 ctgggacatc cgcttaatgc cgccgtctgg ctggcacgca aaatggccag tctgggtgaa
    11401 ccgctgcgcg ccggagatat cattcttacc ggggcattag gtccgatggt ggcagtgaat
    11461 gcgggcgatc gttttgaagc ccatattgaa ggcataggtt cagttgctgc ggcattttca
    11521 agcgcagccc caaaaggaag tctgtcatga gtaagcgtaa agtcgccatt atcggttctg
    11581 gcaacattgg caccgatctg atgattaaaa ttttgcgtca cgatcagcat ctggagatgg
    11641 cggtgatggt cggcattgat cctcagtccg acggtctggc gcgtgccaga cgtatgggcg
    11701 tcgccaccac ccatgaaggg gtgatcgggc tgatgaacat gcctgaattt gctgatatcg
    11761 acattgtatt tgatgcgacc agcgccggtg ctcatgtgaa aaacgatgcg gctttgcgcg
    11821 aagcgaaacc ggatattcgc ttaattgacc tgacgcctgc tgccatcggc ccttactgcg
    11881 tgccggtggt taacctcgag gagaacgtcg atcaactgaa cgtcaacatg gtcacctgcg
    11941 gcggccaggc caccattcca atggtggcgg cagtttcacg cgtggtgcgt gttcattacg
    12001 ccgaaattat cgcttctatc gccagtaaat ctgccggacc tggcacgcgt gccaatatcg
    12061 atgaatttac ggaaaccact tcgcgagcca ttgaagtggt gggcggcgcg gcaaaaggga
    12121 aggcgattat cgtgcttaac ccagcagagc caccgttgat gatgcgtgac acggtgtatg
    12181 tattgagcga cgaagcttca caagatgata tcgaagcctc aatcaatgaa atggctgagg
    12241 cggtgcaggc ttacgtaccg ggatatcgcc tgaaacagcg cgtgcagttt gaagttatcc
    12301 cgcaggataa accggttaat ttgccgggcg tagggcagtt ctccgggctg aaaacggcgg
    12361 tctggctgga agtcgaaggc gcagcgcatt atctgcctgc ctatgcgggc aacctcgaca
    12421 ttatgacttc cagtgcgctg gcgacagcag aaaaaattgc ccagtcactg gcgcgcaagg
    12481 caggagaagc ggcatgaacg gtaaaaaact ttatatctcg gacgtcacgt tgcgtgacgg
    12541 tatgcacgcc attcgtcatc agtattcgct ggaaaacgtt cgccagattg ccaaagcact
    12601 ggacgatgcc cgcgtggatt cgattgaagt ggcccacggc gacggtttgc aggggtccag
    12661 ctttaactat ggtttcggcg cacatagcga ccttgaatgg attgaagcag cggcggatgt
    12721 ggtgaagcac gccaaaatcg cgacgttgtt gctgccagga atcggcacta ttcacgatct
    12781 gaaaaatgcc tggcaggctg gcgcgcgggt ggttcgtgtg gctacgcact gtaccgaagc
    12841 tgatgtttcc gcccagcata ttcagtatgc ccgcgagctc ggaatggaca ccgttggttt
    12901 tctgatgatg agccatatga ccacgccgga gaatctcgca aagcaggcaa agctgatgga
    12961 aggctacggt gcgacctgta tttatgtggt ggattctggc ggtgcgatga acatgagcga
    13021 tatccgtgac cgtttccgcg ccctgaaagc agtgctgaaa ccagaaacgc aaaccggcat
    13081 gcacgctcac cataacctga gtcttggcgt ggcgaactct atcgcggcgg tggaagaggg
    13141 ctgcgaccga atcgacgcca gcctcgcggg aatgggagcg ggcgcaggta acgcgccgct
    13201 ggaagtattt attgccgccg tggataaact gggttggcag catggggccg atctctatgc
    13261 gttaatggat gctgctgacg acctggtgcg tccgttgcag gatcgaccgg tacgagtcga
    13321 tcgcgaaacg ctggcgctgg gatacgctgg tgtttactcc agcttcctgc gtcactgtga
    13381 aacggcggcg gcgcgttatg gcttaagcgc ggtggatatt ctcgttgagc tgggcaaacg
    13441 ccggatggtt ggcggccagg aggatatgat cgtcgacgtg gcgctggatc tgcgcaacaa
    13501 caaataatat tgactgccga gaacgtgcat tttgtatgcc catcccagca cgactgccgg
    13561 atgcggcgtg aacgccttat ccggtctaca tttcgcccgt aggcagtcat taaatagttc
    13621 tgattacgcg gctggcaatg tatcagtcgc gattcacctg cacacgcaat gaggttcatc
    13681 ttatgtcgac tcgtacccct tcatcatctt catcccgcct gatgctgacc atcgggcttt
    13741 gttttttggt cgctctgatg gaagggctgg atcttcaggc ggctggcatt gcggcgggtg
    13801 gcatcgccca ggctttcgca ctcgataaaa tgcaaatggg ctggatattc agcgccggaa
    13861 tactcggttt actacccggc gcgttggttg gcggaatgct ggcggaccgt tatggtcgca
    13921 agcgcatttt gattggctca gttgcgctgt ttggtttgtt ctcgctggca acggcgattg
    13981 cctgggattt cccctcactg gtctttgcgc ggctgatgac cggtgtcggg ctgggggcgg
    14041 cgttgccgaa tcttatcgcc ctgacgtctg aagccgcggg tccacgtttt cgtgggacgg
    14101 cagtgagcct gatgtattgc ggtgttccca ttggcgcggc gctggcggcg acactgggtt
    14161 tcgcgggggc aaacttagca tggcaaacgg tgttttgggt aggtggtgtg gtgccgttga
    14221 ttctggtgcc gctattaatg cgctggctgc cggagtcggc ggttttcgct ggcgaaaaac
    14281 aggctgcgcc accactgcgt gcgttatttg cgccagaaac ggcaaccgcg acgctgctgc
    14341 tgtggctgtg ttatttcttc actctgctgg tggtttacat gttgatcaac tggctaccgc
    14401 tgcttttggt ggagcaagga ttccagccat cgcaggcggc aggggtgatg tttgcactgc
    14461 aaatgggtgc ggcgagcggg acgttaatgt tgggcgcatt gatggataag ctgcgtccag
    14521 taaccatgtc gctactgatt tatagcggca tgttagcttc gctgctggcg cttggaacgg
    14581 tgtcgtcatt taacggtatg ttgctggcgg gatttgtcgc ggggttgttt gcgacaggtg
    14641 ggcaaagcgt tttgtatgcc ctggcaccgt tgttttacag ttcgcagatc cgcgcaacag
    14701 gtgtgggaac agccgtggcg gtagggcgtc tgggggctat gagcggtccg ttactggccg
    14761 ggaaaatgct ggcattaggc actggcacgg tcggcgtaat ggccgcttct gcaccgggta
    14821 ttcttgttgc tgggttggcg gtgtttattt tgatgagtcg gagattaaga atgcagccgt
    14881 gtgccgatgc ctgacgcgcc ttattaagca ggggcgaaag ggacttgccc ccaatacacc
    14941 cacggcgttt acaggtatac tcgctaaaaa ttattcagcg ggtttggaaa caaagatggc
    15001 aaaacttacc ttacaagagc agttgctcaa agcaggatta gtcaccagca aaaaagcggc
    15061 gaaggtcgag agaacggcga aaaaatcgcg cgttcaggcg cgtgaagctc gggcggcggt
    15121 agaagaaaat aaaaaggcac agcttgagcg tgataaacag cttagcgaac agcaaaaaca
    15181 agcggcgttg gcgaaagaat ataaagctca ggtgaagcag cttattgaaa tgaaccggat
    15241 caccattgcc aatggcgata ttggttttaa cttcactgat ggcaatctga ttaagaagat
    15301 ttttgtcgat aagctcacac aggcgcagtt gattaatggt cgtctggcga ttgcccgctt
    15361 gttggtcgat aacaatagcg aaggtgaata cgccattatt cccgccagcg ttgccgataa
    15421 aattgctcag cgtgatgcca gcagtattgt gttgcacagc gcgctcagtg ccgaagagca
    15481 ggatgaagac gacccgtatg ccgacttcaa agtgcctgat gatttgatgt ggtaaacata
    15541 gataagtcgt gcgcagatgc ctgatgcgac gctatgcgcg tcttatcatg cctacggcct
    15601 gcgggcgaaa tgtaggcgga taaggcgttc acgccgcatc cggcagtcgt gcactattat
    15661 caacgcatat tcagtttatt ggcgtggtag gcaatatgct cgccaataaa actggagaca
    15721 aaataatagc tgtggtcgta accctcgtga taacggatta acgttttgat attcatctct
    15781 tggcagatct tttcaagatt ttgagtccgc agctgttccg cgtaaaaatc atcactcaac
    15841 ccctgatcaa ccatgatttc cgcaacgcgt tgaccttgtg aaataagact caccggatcg
    15901 taatccaacc aggcctcttt attttcacca agatatgcgg caaaggcttg ctgtccccac
    15961 ggtacttgcg atggggagac aatgggcgaa aacgccgaga cgctgacata ttcatctggg
    16021 ttacgtaacg ccagcaccag cgcgcccagt ccgcccatag aatgaccaga gatagacttt
    16081 ttggccgttg ccggaaaatg attcatcact aaatccggca gctcgttgcg gatatagtca
    16141 tacattttgt aatgttcatt ccacggcgct tgcgtcgcgt tcaggtaaaa cccggcacct
    16201 tgcccgagat cgtaacggtc ggcatctgcg acatgactgc ctcgcggact ggtgtccggc
    16261 gcaacaacaa taatgttgtg ctcagccgcg taacgctgca cccccgattt agtaatgaaa
    16321 ttctgctcat tgcaggtcag gcccgaaagc cagtacagca ccggtaattt ttcacctgca
    16381 gcttttggtg ggagatagac gccgacattc atttcacatt tcagtgatgc ggaataatgc
    16441 cgatacacat tttgccagcc gccaaagctg gcatgttttt caatgagttc catcagtcat
    16501 tatctcacgg ttaatccccc ggacggctgc ccggagagag tattaagaac cccgtctgaa
    16561 gtggacgggg taaaccagcg ggaaatcagt aacgaattac ggttcgaatg gatttgcctt
    16621 catgcatcag gtcgaaggcg tcattaattt catccaggct catggtatgc gtgacaaacg
    16681 gttccagatc gatatcacct ttcatcgcat cttcaaccat acccggtaac tgggaacgac
    16741 ctttcacgcc gccaaacgcg gaacctttcc atacgcgacc agtgaccaac tggaatggac
    16801 gggtggagat ttcctgaccg gaacccgcga ccccgatgat caccgactga ccccagccgc
    16861 ggtgcgcact ttccagcgcc gcacgcatca cgttgacatt accgatgcat tcaaaggtat
    16921 ggtcgatacc ccatttgttg atatccagca ggacatcttt aatcggtttg tcgtagtcat
    16981 tcgggttaat gcagtcggtg gcaccgaagc gacgcgccag atcgaatttc ttcgggttgg
    17041 tatcgatagc gataatccgt cccgctttcg cctgacgcgc gccctgaacc actgccagac
    17101 caatcgcgcc aagaccaaac acagcaacag aatcccctgg ctggacttta gctgtgttgt
    17161 gtaccgcgcc aataccggtg gtcacgccac agcccagcag gcagacgtgt tcatggtttg
    17221 cttctggatt aatttttgcc agagacactt ccgcgactac ggtgtattca ctgaatgtag
    17281 aacaccccat gtagtgataa agcggctgcc cattgtaaga aaaacgggtg gtgccgtctg
    17341 gcatcaggcc tttaccctgg gtttcgcgaa ccgcaacaca gaggttagtt ttgccagaac
    17401 gacagaactc acactcgccg cactctgcgg tgtaaagcgg gatcacatgg tcgcctggtt
    17461 tgacgctggt tacgccttca ccgacttcaa ccacaacgcc agccccttcg tgaccgagaa
    17521 ccaccgggaa tacaccttcc gggtcatcgc cggagagggt aaatgcgtcg gtatggcaaa
    17581 cgccggtatg ggtgacttta atcagcactt cacctttttt cggtggtgca acgtcaattt
    17641 caacgatttc cagcggttta ccgggagcaa atgcaacggc agcacgtgat ttcatctctc
    17701 gctcttcctc aatatggtaa tagattcagc tatttaagat aggcacgaac cagttcaata
    17761 gtgtcgtcaa cggattggct gacttcgcgg ctgtagcagt catttcggtc aaacgtttcc
    17821 cggatatggc tttcaagcac ttctgccatc agcccattag ccgcgccccg aacggcagcg
    17881 atctgttgga gtatggcacg gcattcggca tcaccctcca gcgaccgttc cagagcatca
    17941 atctgccctc gaatacgacg aactcgagta aggacctttt tcttctcttc cggagtactg
    18001 ggcatttcgc acctcatcat ctgcacgcaa tatactatag gggggtatgc tatatgtcaa
    18061 tgcatacccc cctatagtat ataacaatgg agaataattg ttgtccgaag cctaaccagg
    18121 tgaacggtcc ctcaagacag atggggaccg tttatgtgct atttttatgc cgcatccgct
    18181 aaaacaaaca tcccgtaagg atggatttcg aggtaatagt gctcaccaat atcgggctgt
    18241 aaacgggtgg cgtttacttg taataaaatc tcctgtccat gccactctac ggtcacttcg
    18301 tactgcggcc ccatgtaggc gacatggcga ataacacaac gctgactctc ttcgccgtga
    18361 ttgcttaagg tgatggcttc cgggcgaacg ccgacggtgc cgctaccttg caccgggaaa
    18421 tgcgccgcgc gcggcaggcg ataaccgtag atatcgacat actcttcgct aaagttcgcc
    18481 gggaacaggt tggcgtcgcc cataaaactt gccataaagc gggaggctgg ctgacgatag
    18541 agatcctgcg gtgagccaat ctgcatgatg tgacctttat tcatcaccag cacagtgtcg
    18601 gaaaccgcga aggcctcact ttgatcgtga gtcacgtaga gcgacgtgat attaaactgc
    18661 ttttgcagct cgcggatctt atcgcgcatg ctgcggcgca ggttggcatc gaggttactt
    18721 aacggctcat caaacagcag cacctttggt ttgaggatca acgcgcgggc cagcgccacg
    18781 cgctgttgct gaccaccgga aatctggtcg acatagcggt cctcgaaccc ttccagatcc
    18841 accattgcca gcgcctcttt cacccgctgc tttacttcgc tacgcgacac gccgagcatc
    18901 tttaacccgt agccgacgtt ttcgcccagc gacatatgcg ggaacagagc gtatgactga
    18961 aacaccatgc agatatcgcg ctgctgaatg gaacgatgcg tgacatcttc gccatcaata
    19021 aagatttgcc cttcactcgg tttttccagc ccggcaacca gacgcaaaat ggtggttttg
    19081 ccgcagccgg aagggccaag cagcgtcacc atttgccctt gtgggatggt gagattgata
    19141 ttgtcgataa ccgtattact gccgaatcgt ttagtgacgt tgcgcagttc cacaaaattt
    19201 ttctgactca tagtgcgctc cattacgcct ggtttttggc tttagaacgg gagatacgtg
    19261 cttctccgat cagccagtca aagaggaaaa taatcgccag catcaccacg atcagaatcg
    19321 aaccataggc gatggctacg ccatattcgc cgtcttcaac gcggttaaga atgtaggcgg
    19381 ttgccacgcg ggtgtccggc gtgacgagaa agacgatggc gctgaccgtc gtgatggccc
    19441 gcacaaagct gtagatcagg gccgagagaa tggccggacg cagcagcggc agtaaaatgt
    19501 gggttatggt tcgcaaggaa cccgcgcgca ggctgagtga ggcttcatcc agcgatttgt
    19561 cgatctgccc caggcctgca atcccggcgc gaatacccac cggaacgtta cgcatcacca
    19621 tcgagataat cacaattgcc gccgttccgg tgagataaac cggagcgctg ttaaaggcaa
    19681 gaatatacga aacgcccgcc accgtacccg gtacggcaaa gcacagcatg gtggtgaact
    19741 cgatggtctt tttgcctttg aattgttgac gcaccaccac ccaggcgatg agcaggccaa
    19801 agatagcggt aatgggagcg gcaatcccgg cgtagagcag ggtatccagc agtgaaggcc
    19861 acgcgccgtc gctcattccc tgaccaaata gcttgataaa gttatccagc gtcagggtgt
    19921 agtccacgcc ccagttcacc gtgaagctgc cgtagaaaat gctgccgtag agcagggcgt
    19981 taaaggcaat ccatatcgca agtatggcga ccacgctcca caccagggtg accggaagcg
    20041 gctggacgtc gccacggtag gatttgccgg aaaccgtgac gtaagagcgt ttaccaatcc
    20101 acatatactg tatgcagaac accagcagcg agaacagcag caggaaagcg ccaagggtgc
    20161 tggcggcctg ataatccagc tgcgagccgg taatatagaa gtaaatttgc gtcgccagta
    20221 cgtcgaagtt accgccaagt accagcgggt tactgaaatc agccagtgac tgtaccacga
    20281 cgattaaaaa ggcattggcg agcgcgggct tcaacaacgg cacaaacacg ccgttaaagg
    20341 tttgccagcg gctggcgcgc agggtgtagg aggcctcttc cagcgaggga tgaatggttt
    20401 taatggcccc gtcgaggatc ataaatgcca ttggcgtgaa tgccagaacc tgtgccagcc
    20461 agatcccggt aaaaccgtac agccagttgg tgttggtcag cccaaaccac tccaccataa
    20521 attccgtaac atagccggaa cgccccatca tgagcgtcac gcctaagccg accacaaacg
    20581 gtggcgtaac gatgggcaaa atggaaaaga tgcgaccaat aatggcgctg cgtttagcga
    20641 tgcgcgtggt ataaatcgcc agcaccaggc cgaagaaagc acagcctatc cccaccgcta
    20701 tcgacaagac gatagagttg acgatcacct gaacaatatg tgcctgcgag agcacggtca
    20761 taaacgccag cggggcgagc tctcccgcgt cgttggtgaa catcgggata aaaatggcga
    20821 tgctcggcca gacgataaaa atgccaatca gcgccacaat cgcaatcagt gagccgatca
    20881 cgaagcggtc gccgcccagc cattcgaggc gggttaaggc cagcgtcata atcgcgccta
    20941 gttcgacaaa cagcacgatg gcggcgtagc ctaaaccgcg tccagccacg gtggcgctga
    21001 tgacgataaa cgccatacag agcaatgccc agccggcatc gaaataatga cgactacgct
    21061 gttcgcgcgt ggcctgacga aacgggcgga ccagcaacag gctcgggagt aaataccaca
    21121 gccagctgat attaaaatgc gaccagccgt acgccccgag gatctcatca ctcgtggatt
    21181 ccagcaggcc ataatcgagg ctccagctcg gcaacaggac aaatgccagc cagcccagca
    21241 aaacccaaag gaaaatcgca tcccgctttt tcacgggatg gagtgcaagt gtctgtgaca
    21301 tagcagttcc ggtatggttg gccggggcag gcgtctgcgc tgcacccggc aaatgcgcct
    21361 aatgcgggtt tatttaccca tcttcacttc gctgacccat ttattaatca atgccttacg
    21421 aacatccgtg gaaccatatt tatccatgtc gtagttgatt aatttcaggt cgtcgagctt
    21481 cagcgaattt ggcgacgttt cggcggtggt attagtcagg atctgataag acttcccttt
    21541 cttccacgcc agttcctgag cttcttttga taatacccag tccacaaaca gtttggcgtt
    21601 gtcgaggttg cgcgcgcctt tcagaatgct gacgccgcca atttcgtagc ccgtaccttc
    21661 acacggggaa atcaactcca gcggcgcgcc ctgttctttt tccagcgagt agtcatgcag
    21721 gaagccaata ccaatcgccg tttcgccacg ggcggcgtta cgtgccgggg ctatgcctga
    21781 tttggtgtac tgagagacgt tggcgttcag ttgttttaga taatcaaagg cctgatcttc
    21841 cccccacagc tgcgcaaaag tagccagggc ggtgtaggca gtaccggaac tttgcggatc
    21901 ggcaatttga atttcgcctt tatattccgg tttggtgaga tctttccagc acttcggcac
    21961 cggcaggttt ttctctttca gacgatcggt gtttacgcca aaaccgagga tccccacata
    22021 aacggcagag gacagattcc ctttcacttt tgccggatcg cggaatttct ccatgatctg
    22081 ctcaaggtta ggggatttat acgcctgtag caggcccatc tcgccagcct gcgattgcgg
    22141 gtctaaagtg ccgccgtacc agacgtcagc ctgggggttt ttcttttcag catcgacttt
    22201 tgccagggtg cttccggaac cgttgcggat aaacgaggtt tttacatcat atttctcgcc
    22261 aaacgctttg gtttcggcct cacacatttc attggtggcg ctgcaataga ccaccagccg
    22321 gccttttgcc tgcgcggcac ccgttaaagt cgccagcgca acggcagaag caatcagagt
    22381 agaggtaaag gtcattttca ttgttttatc cttcgagcgt gagtggtggt gatgcccgcc
    22441 atcagtaacg gcatcagcag taaacccata agtactgcgg cgaccgagag taaactgaac
    22501 attcccggcc agccgtagcg ttcgatgacc agcgacagcg gccagcctgc cagcgccgcg
    22561 ccaaggtaag cgaacaggcc gagaaatccg gtgattgaac ccgccgcagc tttgtgccca
    22621 cactccaccg ccgcaagtcc aatcagcatt tgtgggccaa agacgaaaaa gcccacggta
    22681 aaaaagcaca ctgccagcag agcgtagtga tgaaccggtg ccagccacag tgcggcgact
    22741 gacaccatca accccaacgt gaacagcaaa atcattggcg ctcgttgccc gctaaacagc
    22801 aaatccgatc cccatccggc aaataacgcg ccgagtaagc cgcccacttc aaacagcatt
    22861 accgtggcgt tggcgctgag caggttgaca ccgtggcttt ccgtcagcca gatattgccc
    22921 cagtcgttaa gcgcaatccg gatcacataa accagtacat atgagacgcc cagtagccag
    22981 atcagcggat tttgcagcat ggtggtgcgc aacatttgcc acagccccat cggcggactt
    23041 tgttgttcct gacgtaactc cagcggatcg tgacgccagt gaccaactgt cggtaatccc
    23101 tcttcctggg gcgtgccttt gagttgtagc gttaaccaga tccctaatgc catgctgatt
    23161 atccccggtg tcagcatcgc ggcctgccag ccccaccagt gggcggcaaa ggcgctaatc
    23221 agcggaataa tcgctccgcc aatgttgatc gacatattcc agcagcccca ccagaaaccg
    23281 cgctcattgc gtgagtacca gtgggtcagc aagcgggcac acggtggcca tccccagccc
    23341 tgaaaaaaac cgttcagtgt ccagaccaca agcagaagcg taagtgattc gccaaaggca
    23401 aacaccacgt tcaataaccc ggtggcgaac aagccgacgc ccataaaacc gcgttgtccg
    23461 tggctgtcat gccacagtcc ggcggtaaat ttcgacaatc cgtagctgag ataaaacagc
    23521 gaacccagca ggccaatgtc gcctttatcc agtccgaggt cggtttgtag cgccggaagg
    23581 acgtaattca cgctttttcg cgtcagataa aatgcggcgt agccaataat catgcacatc
    23641 agcaatcgcg gacgtaatgt ccggtagcat tgattaattt ctctcgctga acgcgcgtgc
    23701 atggttgtct ccatgttgct gactatatga aaatgaaagt gaaaggggat gagagaaaat
    23761 ccgggatacc taagactttt tcctggttat ccggcgattt gttgcaaaaa tgtgggcaag
    23821 ttaacaatta cccgcgtgcc gtgctgtttt tcgagcgtta agtcgccgcc cagggcgctg
    23881 acgcgctcac gcatcccctg aatgccaaat ccagccattt tcccgctggc gatccccaca
    23941 ccgttgtcgc tgacttccag atgcaatacc tctccctgct ggcgcaggat aatcgtcact
    24001 tcgctggctt cggcgtgttt acagatgtta ttgagcaact cctgcaataa ccgatacaag
    24061 gtaaagcgca ccgtttcgtt ttctggtgtg gagttcagtt gataggcaaa ctggcaatga
    24121 atgccgcgtt cactaaaggc aaattcatta atcagatgca gcaacgcttc gcgaaatgtc
    24181 agttcatcaa gcgcgggagg ccgcaactgg cgcagcaatt ggcgggtgga gagatgaatg
    24241 cggcgggcga gctcattgat ttggctggct gcggattgaa tttgcgtcgg atcacgcgcc
    24301 cgcttcacca gttgcgactg gatctgaatg gcggtgatgt tctgaccgat ttcgtcgtgc
    24361 agctctcgcg ccaggttttt tcgcgtgtcc tcctcggtgt ggatcagctt ttccgccaat
    24421 gcccgccgcg ctgccagctc ggcctccagc cgttgtcggt aatggtgcag gtttagcgcc
    24481 agatgttgct ggcgactgat ggcaatgccc aggccaattc ccagcaaggc ctgggtcgcg
    24541 aggaaaatct ccagctccac caggttacta aagcccacgc caatctggcg ggcgatggtg
    24601 atcatcatgc tgcccaaaag cccggagagt acgccacctt gccagccaaa tttccacgcc
    24661 atcaccacat tgggcaaaaa gaccacgatc aacagcagac gttcaatttc aggtgacagc
    24721 accatctgag tgccgatacc aataataaaa aacaggctac accagatgat taaagaggta
    24781 cgcaaggggg gattatgcgt atcgagtccc aggagctggt agcgatgctg ctggcgcaga
    24841 aactcaaaga taagatagac aaacggcgtc agtaatacgc cgccggtaaa tgaggtcaaa
    24901 cccggtagca ttattgggcc tgacataaaa ggggagagca gggcggtatt caacaatgaa
    24961 gctgccgtga cagtggctaa cagcaacgtc agacgctgcc agtaaagtgg aaaacggtgc
    25021 cagaagtttt gcgcaaggag ggcaggaaac aggctaataa ccggcgttgc cagcatgaga
    25081 taaccgttca atagctgttc actatggagc cagaacatca tcagcaaagg ggggagcacc
    25141 aacgcaggcc agtagcggcg agagagcaaa ataacaaggg cgagataaac tccctgcggg
    25201 agaaacaaca ccgcctgttg accgttattg gtcagataaa agctgagcgt ccacagcatc
    25261 agccagccgg ttccccaggc tagcacaata aacagcaaaa tcacccaatg acgaagattg
    25321 cgcgacatta atgccccgcc agcagatggt ggtcgagcgc aaaatgtacc agttcaatgg
    25381 tgctatggca gttcagtttt cccagcacat tggcgcgatg cacatgtacc gttttatgac
    25441 tgagttcgag tttaaaggcg atctctttaa cgctgtctcc tttgaccagt aactcaaaca
    25501 cttcacgttc tcgtggggtg agaatttcca gcgcctgggc gggttgcccg ccgccgcgta
    25561 aagcccgaat cgcatcggca cagagataat gcccgcccag cccgaccgag cgcaccgcct
    25621 gtactaactc ttccggccca cagcgtttgg ttaaataccc gctggcaccg gcatccagcg
    25681 cgctttgcac aaaggttggc gcgtcataaa tgctaagaat aatggcgcga aattgcggtt
    25741 tttgtgcacg caggcgctta agcaaactga ggccgttttc gtccggcatg gcaatatcaa
    25801 tgacggcaac attgacgtca tcacggatta atgccgacca tgcctgcgcg gcactactgt
    25861 actgaccaat gacctccaga tcgtcttcca gagagagtaa ttgtgcgaag ccagagcgca
    25921 ccacaacatg gtcatccacc agcactaccc gaatcatact tttcttctct tattatctaa
    25981 ttctcctatc atgcatcacg gcgcggcgct ggcaatcaac cagcgacaga tatggaatgg
    26041 atgtaacaaa atggcaataa tttgccggag cgcgcacatc ttatctggct tgtgaaccgg
    26101 cgggatacgt tcgccaaaac cgcaaaatgc gcaaactcgc taaccattgc cgattttttt
    26161 ctaaaaacgc tttgttctta acaagacctt ttaattcctt tatcaaactg acccgcaacg
    26221 aaatactgct cccataacaa gacaggggag cagacaatca tggcaatttc atcgcgtaac
    26281 actcttcttg ccgcactggc attcatcgct tttcaggcgc aggcggtgaa cgtcaccgtg
    26341 gcgtatcaaa cctcagccga accggcgaaa gtggctcagg ccgataacac ctttgctaaa
    26401 gaaagtggag caactgtgga ctggcgcaag tttgacagcg gagccagcat cgtgcgggcg
    26461 ctggcttctg gcgacgtgca aatcggcaac cttggttcca gcccgttagc ggttgcagcc
    26521 agccaacagg tgccgattga agtcttcttg ctggcgtcaa aactgggtaa ctccgaagcg
    26581 ctggtggtaa agaaaactat cagcaaaccg gaagatctga ttggcaaacg catcgccgta
    26641 ccgtttatct ccaccaccca ctacagcctg ctggcggcgc tgaaacactg gggcattaaa
    26701 cccgggcaag tggagattgt gaacctgcag ccgcccgcga ttatcgctgc ctggcagcgg
    26761 ggagatattg atggtgctta tgtctgggca ccggcggtta acgccctgga aaaagacggc
    26821 aaggtgttga ccgattctga acaggtcggg cagtggggcg cgccaacgct ggacgtttgg
    26881 gtggtgcgca aagattttgc cgagaaacat cctgaggtcg tgaaagcgtt cgctaaaagc
    26941 gccatcgatg ctcagcaacc gtacattgct aacccagacg tgtggctgaa acagccggaa
    27001 aacatcagca aactggcgcg tttaagcggc gtgcctgaag gtgacgttcc ggggctggtg
    27061 aaggggaata cctatctgac gccgcagcaa caaacggcag aactgaccgg accggtgaac
    27121 aaagcgatca tcgacaccgc gcagtttttg aaagagcagg gcaaggtccc ggctgtagcg
    27181 aatgattaca gccagtacgt tacctcgcgc ttcgtgcaat aaaaggaggc gcagatgctg
    27241 caaatctctc atctttacgc cgattatggc ggcaaaccgg tactggaaga tatcaacctg
    27301 acgctggaaa gcggcgagct actggtggtg ctggggccgt ccggctgcgg taaaaccacc
    27361 ctgctgaatt tgattgccgg ttttgtgcct tatcagcatg gcagcattca actggcgggt
    27421 aagcgtattg aggggccggg agcagagcgt ggcgtagttt ttcagaatga agggctacta
    27481 ccgtggcgca atgtacagga caacgtggcg ttcggcctgc aattggcagg tatagagaaa
    27541 atgcagcgac tggaaatcgc gcaccagatg ctgaaaaaag tggggctgga aggcgcagaa
    27601 aaacgctaca tctggcagct ttccggtggt caacgtcagc gggtggggat tgcccgcgcg
    27661 ctggcggcga atccccagct gttattactc gacgaaccgt ttggtgcgct ggacgccttc
    27721 acccgcgacc agatgcaaac cctgctgctg aaactctggc aggagacggg caagcaggtg
    27781 ctgttgatta cccacgatat agaagaagcg gtgtttatgg cgactgaact ggttctgctt
    27841 tcacccggcc ctggccgcgt gctggagcgg ctgccgctca actttgctcg ccgctttgtt
    27901 gcgggagagt cgagccgcag catcaagtcc gatccacaat tcatcgccat gcgcgaatat
    27961 gttttaagcc gcgtatttga gcaacgggag gcgttctcat gagtgtgctc attaatgaaa
    28021 aactgcattc gcggcggctg aaatggcgct ggccgctctc gcgtcaggtg accttaagca
    28081 ttggcacgtt agcggtttta ctcactgtat ggtggacggt ggcggcgctg caactgatta
    28141 gcccgctgtt tttgccgccg ccgcaacaag tactggcaaa actgctcacc attgccggac
    28201 cgcagggatt tatggacgcc acgctgtggc agcatctggc agccagcctg acgcgcattg
    28261 tgctggcgct actcgcggcg gtgttgatcg gcattccggt cgggatcgcc atggggctaa
    28321 gcccgacggt gcgcggcatt ctggatccga taatcgagct ttatcgtccg gtgccgccgc
    28381 tggcttattt gccgctgatg gtgatctggt ttggtattgg tgaaacctcg aagatcttac
    28441 tgatctattt agcgattttt gcaccggtgg cgatgtcggc gctggcgggg gtgaaaagcg
    28501 cgcagcaggt tcgcattcgt gctgcccagt cgctgggtgc cagccgtgcg caggtgctgt
    28561 ggtttgtcat tttgcccggc gcgctgccgg aaatcctcac cggattacgt attggtctgg
    28621 gtgtgggctg gtctacgctg gtggcggcgg agctgattgc cgcgacgcgc ggtttaggat
    28681 ttatggttca gtcagcgggt gaatttctcg cgactgacgt ggtgctggcg gggatcgcgg
    28741 tgattgcgat tatcgccttt cttttagaac tgggtctgcg cgcgttacag cgccgcctga
    28801 cgccctggca tggagaagta caatgagtga acgtctgagc attaccccgc tggggccgta
    28861 tatcggagca caaatttcgg gtgccgacct gacgcgcccg ttaagcgata atcagtttga
    28921 acaactttac catgcggtac tgcgccatca ggtggtgttt ctgcgcgatc aagccattac
    28981 gccgcagcag caacgcgcgc tggcccagcg ttttggcgaa ctgcatattc accctgttta
    29041 ccctcatgcc gaaggggttg acgagatcat cgtgctggat acccataacg ataatccgcc
    29101 agataacgac aactggcata ccgatgtgac atttattgaa acgccacccg caggggcgat
    29161 tctggcagcg aaagagttac cttcgaccgg cggcgatacg ctctggacca gcggtattgc
    29221 ggcctatgag gcgctctctg ttcccttccg ccagctgctg agcgggctgc gtgcggagca
    29281 tgattttcgt aaatcgttcc cggaatacaa ataccgcaaa accgaagagg aacatcaacg
    29341 ctggcgcgag gcggttgcga aaaatccgcc gttgctacat ccggtggtgc gaacgcatcc
    29401 ggtgagcggt aaacaggcgc tgtttgtgaa tgaaggcttt actacgcgaa ttgttgatgt
    29461 cagcgagaaa gagagcgaag ccttgttagg ctttttattc gcccatatca ctaaaccgga
    29521 gtttcaggtg cgctggcgct ggcaaccgaa tgatattgcg atttgggata accgtgtgac
    29581 tcagcactat gccaatgccg attacctgcc acagcggcgg attatgcatc gggcgacgat
    29641 tcttggggat aaaccgtttt atcgggcggg gtaatacgag agtggacggt cctctcgccc
    29701 ccttggggag agggttaggg tgagggggcg ttcaccgtgc tttcaacagg ttaactcccc
    29761 ctttctgaga ggaaacaaaa ttaacgcaga atcttcttct cagccaaatc cagcgcaaag
    29821 tagctgaaaa tcagatccgc acccgcacgc ttaatcgaac ctaagctttc gagcacgact
    29881 ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcata ctcaccgctc
    29941 acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac gatgtcgagg
    30001 tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc cagcagtgac
    30061 tcacgaatcg cctcacgacg gttcattggg ttcatctgat agcttttgcg gtcgcctttt
    30121 aatgcgcttc cggcagcttc acggaacgga ccataaaagg aggaggcgaa cttggtcgaa
    30181 tacgacataa tcgccgtatc tttaaagcca gcagcgtcca gcgcctggcg aatcgcctgt
    30241 acctggccat ccatcgcggc ggaaggggcg atgaagtctg cacctgcagc agctgcaacc
    30301 acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg ctcgcacagc
    30361 acaccgcagt gaccgtgaga agtgtattca cagaagcagg tgtctgacat gacaatcatt
    30421 tctggcaccg tctgcttgca gatgcgcgac atacgcgcta ccagtccatc ttcccgccag
    30481 gcatcgctgc cggtttcatc ggtatggtga gagatgccga aagtcatcac ggaacgaata
    30541 ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat gcgcatcacg
    30601 ccaggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa gatcggcaac
    30661 accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc aggagatttg
    30721 cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt tgtggaatta
    30781 aggtgcacag tatacctgaa gcggggctgg agtgttttac gaaagttgtc acgatgttga
    30841 caagaagaga atgaaagaga gaccagggca tctttgtaat agaaatagca ataacttcat
    30901 attcact
//
LOCUS       DAZGXS010000045        30620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGXS010000045 DAZGXS010000000
VERSION     DAZGXS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHKG5_002172"
     CDS             complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHKG5_002172"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0868277.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRAT"
     gene            complement(3868..4086)
                     /locus_tag="ACHKG5_002173"
     CDS             complement(3868..4086)
                     /locus_tag="ACHKG5_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFP0868278.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ACHKG5_002174"
     CDS             complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ACHKG5_002174"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0868279.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5534..7774
                     /gene="fepA"
                     /locus_tag="ACHKG5_002175"
     CDS             5534..7774
                     /gene="fepA"
                     /locus_tag="ACHKG5_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP0868280.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7949..8569
                     /gene="entD"
                     /locus_tag="ACHKG5_002176"
     CDS             7949..8569
                     /gene="entD"
                     /locus_tag="ACHKG5_002176"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0868281.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8691..8843)
                     /gene="hokE"
                     /locus_tag="ACHKG5_002177"
     CDS             complement(8691..8843)
                     /gene="hokE"
                     /locus_tag="ACHKG5_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0868282.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9194..9346)
                     /gene="hokE"
                     /locus_tag="ACHKG5_002178"
     CDS             complement(9194..9346)
                     /gene="hokE"
                     /locus_tag="ACHKG5_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0868283.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9315..9582
                     /locus_tag="ACHKG5_002179"
                     /pseudo
     CDS             9315..9582
                     /locus_tag="ACHKG5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9785..10903
                     /locus_tag="ACHKG5_002180"
     CDS             9785..10903
                     /locus_tag="ACHKG5_002180"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0868284.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10969..11217
                     /locus_tag="ACHKG5_002181"
     CDS             10969..11217
                     /locus_tag="ACHKG5_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0868285.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11282..11650
                     /locus_tag="ACHKG5_002182"
     CDS             11282..11650
                     /locus_tag="ACHKG5_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0868286.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11744..12397
                     /gene="nfsB"
                     /locus_tag="ACHKG5_002183"
     CDS             11744..12397
                     /gene="nfsB"
                     /locus_tag="ACHKG5_002183"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0868287.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12505..13752
                     /gene="ybdG"
                     /locus_tag="ACHKG5_002184"
     CDS             12505..13752
                     /gene="ybdG"
                     /locus_tag="ACHKG5_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0868288.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13820..15196)
                     /gene="pheP"
                     /locus_tag="ACHKG5_002185"
     CDS             complement(13820..15196)
                     /gene="pheP"
                     /locus_tag="ACHKG5_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0868289.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15298..18435)
                     /gene="cusA"
                     /locus_tag="ACHKG5_002186"
     CDS             complement(15298..18435)
                     /gene="cusA"
                     /locus_tag="ACHKG5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0868290.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18447..19670)
                     /gene="cusB"
                     /locus_tag="ACHKG5_002187"
     CDS             complement(18447..19670)
                     /gene="cusB"
                     /locus_tag="ACHKG5_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0868291.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19686..20018)
                     /gene="cusF"
                     /locus_tag="ACHKG5_002188"
     CDS             complement(19686..20018)
                     /gene="cusF"
                     /locus_tag="ACHKG5_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0868292.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20042..21424)
                     /gene="cusC"
                     /locus_tag="ACHKG5_002189"
     CDS             complement(20042..21424)
                     /gene="cusC"
                     /locus_tag="ACHKG5_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0868293.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21581..22264
                     /gene="cusR"
                     /locus_tag="ACHKG5_002190"
     CDS             21581..22264
                     /gene="cusR"
                     /locus_tag="ACHKG5_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0868294.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22254..23702
                     /gene="cusS"
                     /locus_tag="ACHKG5_002191"
     CDS             22254..23702
                     /gene="cusS"
                     /locus_tag="ACHKG5_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0868295.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
     gene            24296..26197
                     /locus_tag="ACHKG5_002192"
     CDS             24296..26197
                     /locus_tag="ACHKG5_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0868296.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            26225..26686
                     /locus_tag="ACHKG5_002193"
     CDS             26225..26686
                     /locus_tag="ACHKG5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0868297.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            26706..>30620
                     /locus_tag="ACHKG5_002194"
     CDS             26706..>30620
                     /locus_tag="ACHKG5_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0868298.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISE
                     ETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYR"
BASE COUNT         7360 a         8336 c         8079 g         6845 t
ORIGIN      
        1 gcgttgcgcg aataatcggc ccaatttttt caaatgcccc tggagagata atatccacat
       61 gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag gcgcgttcgg
      121 ggctcatacc ttcctgaagc gtacgttcag caacaaacag cgtcgctttt ccgtcaaacg
      181 gtacgctatg agcagtcgtc agcaggcgca cagcatcagc gtagttgcct tcaatggtgg
      241 taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg cgctcgcggt
      301 taatctccgc cagcacttcc gggtccagac cattagcttc tttttcctgc cagttttgcg
      361 tttctggcgg ccaggtatcc agcaagccaa gaaatgccac ctgttcgcca cgggcacgta
      421 gtcgcgccgc aatgccctgc gccagtgtac cgccaaggga ataccccagc aggtaataag
      481 ggccgtgcgg ttgttgttca agtaacgttg ccagatgcgc ttcgcagact tcatccaggt
      541 tcgtcgctgt ctgcatgggg ccatgagggc gcggcgactg aatgccgata atcgaccata
      601 gtggatcgag ataacgcgag agcacgctaa actgccaggc aaaaccggat gccggatgga
      661 aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg aatcccatgc
      721 gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg gttgacgcga
      781 ccatcacctg ccccggcgtc acctgacggg caaactgccg acttaactgc gctgccagtt
      841 tcattgccag tagcgaatga ccgccaagcg cgaagaaatc agcatcggca tcctgcacgt
      901 cacaacccag caacgacgcg aatgccgcag cgataatcgt ttcactgccc gctttcggcg
      961 cacgccccgg cgtttgtgcc ttaagttcag gcaacggtaa ggctttgcga tccagcttgc
     1021 cgttggcgct aagaggtaac tgtggaagtt gcagcagaac gaccggcgcc atatgcggcg
     1081 gcaatgtttc gcgaagctgt gcctgtaatg cgctggtatc caacggcaga cctgattgcg
     1141 acaccagata gcccaccaac tgacgcgcat caccaccggt ggctgccgcc tggttaatca
     1201 cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc ctgcatcacg cgatcgattt
     1261 cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc ccgaggtact
     1321 ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt acggtacatc cgttcaccag
     1381 ggacaaaagg atcggcaatg aagcggctgg cggtcagatc gggtcgtcca agatacccct
     1441 gcgccagttg aataccggtg agatagagat ctcccgccac acccggcggc accggatgca
     1501 tcatcgcatc gagaatgcgc aagcccgtat tccacaccgg ataaccaatc ggcacactgc
     1561 tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccaactcaca tctaccgccg
     1621 cttccgtcgg gccatataga ttatgcaacg gcgcgcccgt taactgttgc cattcgcggc
     1681 ataaatcagc cggtaaggcc tcaccactac agaaaacctg tttcaacgtc gcgcaattct
     1741 ggcgagcggt ttgcggcgtc agcgatgcaa caaatgccgc cagcatcgac ggcacaaagt
     1801 gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg tcgcggtgcg
     1861 cttccggttc agccatcacc agtttagccc cggcaataaa cggccagaaa aactcccaca
     1921 ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcacct gtaagtggat
     1981 aatgattttg catccacaac aggcggttaa cgatagccgt ctgcccgacc attacccctt
     2041 tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtgatgc ggttgtgaaa
     2101 gttgcagcgg cgcactgccc tgcggtgtaa gcggggcgtt atagcaaagg ttggttaaat
     2161 ctggaacatc ggcaaagcgc ggcagttgat cgtcggtggt gattaacagc gacggacgcg
     2221 catcttccag catcattttc aggcgatcgt ccggataacc ggtatccagc ggtaaccagg
     2281 ccgcacctgc ttcaacaatc gcatgtagcg ccagggtcaa aaagaccgag cgcggtaatg
     2341 ccaccgccac gctgtcccct ggtttaacgc cgtgctcacg cagcagattc gccagcgcca
     2401 ccacctgctc gcgcatttcc cgatagctga actggtaacg cgcatctgcc agcgccggag
     2461 catccggtgt ttttgccgct tgttctgcca ccagcgcgct aagcgtggtt tctggaatct
     2521 caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc ataatatcaa
     2581 catcgccgca caacagagcc ggatccgcag cgaattgggc gatcagcatt ttcaggcgtt
     2641 cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg atctcaatac
     2701 tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc ggaccggttg
     2761 ccagggtatg ggtttgcgcc tgaacaccag gaatatccag ttggtaatca aataccttga
     2821 tattgagtac cggaccaaac agcggttcat cacctgccgc tcgcccgctg tcacggacaa
     2881 tttgttcggc atcgtaacgt tgatgacgac gcattttttt cagttgtgct gccagtcggg
     2941 ttgccagttc cggcagcgtt tcttgtgccg caatgtgaat acccaacggt aaaacgttga
     3001 gcacgggtcc ggtagccgtc agcgccgccg agcccagtcg acgcataaag ataaatccgg
     3061 cggcgtagtc catgcgattg cacaatcgcc ccagccaaaa ggctgccagc gcaagggcta
     3121 aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc ccgtcggtaa
     3181 attccagttt caggcgtaga atatctgccg aagcgctgcg ccccgctaaa ggtgccggag
     3241 aaagtgacgc gggcggcggc agttgacgac gctgttccgc ccagaatgcc gcatcacgct
     3301 gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg aaaggcgtaa
     3361 acggcgaagc aggcgtttgt tcgccacgca gcaatgcgca gtaaatgttg gcgatctgcc
     3421 gggtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc tgataccagt
     3481 accagcggtt atctgccacc tgtatcagct ggtgaaagac cagtggttta ccgctatcga
     3541 cgcgcaaatc ttgttgcaaa tccgcctgca ttaatgcctg cgcagtaccg tgcggatcaa
     3601 tattggttcg caggtcgata atttctggca gttcgaatat cagcgcatca tcgacccatt
     3661 gccagacttc gccgttatct tccgtaaaac gcatccgcag cgtatctgct tgcgctaatc
     3721 cggcaaccac cgcgcgggcc agtaatggcg catcaacctc tccggttaac tcaacgtaat
     3781 gcgccacgct ccaggcggag ggtaattctg acagtttttc tgccatccag atgccgggct
     3841 gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg tgaaattcgc
     3901 cggtgtcaga gtacgccagt ggacttccag ccactgctgg caggactctt gtgactgcgg
     3961 ctgacacaca atgtcccagc ctgccggtaa ggcgcattgt tgcggccaca gactgaattg
     4021 cccctgcgca ttgcgcaaaa tgtaaaacac tccctgtgga tcatcgaagg gattactgaa
     4081 tgccatattc aactcctgtc atggaaaagt ggttgccaga ggtcgattaa cccctgcatc
     4141 aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca gaaaatggat
     4201 tcttttatag ggtgtaattg cgcatacagc gcctgattgg cccgcatgat catcggctcg
     4261 cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc agcttttagc
     4321 ttttcaagta acacgccctc ttgctgcccg ccccgatgcg gccaccagta cgatcctgac
     4381 tggcttaata cacagccaaa gcgttcaggc cagtgcagtc cggcatacag cgcggacagc
     4441 ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc gatcgctaaa aggggcaata
     4501 gctttcacca ggggtaataa ctcttgctgt actgccagcc agaaatccgc attacacggc
     4561 agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca acacatacac ggcgggagga
     4621 agttgctgac gatgggtcag cgaaatcagc acaggccaga cgggcatact ttgcgcccaa
     4681 aattcgccat cgagcaaaac tgccagcggg cgttcttctg ctgttacatc gccggtggta
     4741 aaaatccata cacgacgtga atttttcaac cgttcacttt tccagataat ttctttggct
     4801 ggtatttctg gcgcttgcgg acaatcccat cccggttgca gaggcgcttg cggcatttcg
     4861 agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt gtggattcag cggatcggct
     4921 attgcctggg gtaatagttt tcgccagcct tcacgtaatt cgaggcgatc gggggatggt
     4981 gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt agctgccgcg ccagttggca
     5041 ttgagttgtg tcgtccactg ccagacatca gtacctgcaa ttcgctgcat cgactggggc
     5101 tggctgttct gatgatgatc ggtcacacca gtgatgtaga tccatacgcg ctttatcgtc
     5161 gagtattctt cagaaccttg gggatcacgc caccagaaag tgacctcaaa catttcgtca
     5221 ttcagacgct gccattccgg gccatgtttc gactgccacc agctctcact tcctactttt
     5281 aacgccgtca caccataacc ccatgtttac tgtgcaattt ttcattgatt gcagaaatat
     5341 attgataata ttattgataa ctatttgcat ttgcaatagc gtaatggcgc gccgtgggaa
     5401 gcgcggacat taattaacca actgcactgc gtgtctttca ggatcaaagg ttttcgcggt
     5461 agcgggatgc gtcgtgttga tgacgaccat gcccgacagt tgcaattcgt ggcaaaaatg
     5521 caggaataaa acaatgaaca agaagattca ttccctggcc ttgttggtca atctggggat
     5581 ttatggggta gcgcaggcac aagagccgac cgatactcct gtttcacatg acgatactat
     5641 tgtcgttacc gccgccgagc agaacttaca ggcgcctggc gtttcgacca tcaccgcaga
     5701 tgaaatccgc aaaaaccccg ttgcccgcga tgtttcggag atcatccgta ccatgccagg
     5761 cgttaaccta accggtaact ccaccagtgg tcagcgaggg aataaccgac agattgatat
     5821 tcgcggtatg ggtccggaaa acacgctgat tttgattgac ggcaagccgg taagcagccg
     5881 taactcggtg cgtcagggct ggcgtggcga gcgcgatacc cgtggtgata cctcctgggt
     5941 gccacctgaa atgattgaac gtattgaagt tctgcgtggt ccggcagctg cgcgttatgg
     6001 caacggcgcg gcgggcggcg tggttaacat cattaccaaa aaaggcagcg gcgagtggca
     6061 cggctcctgg gacgcatatt tcaatgcgcc agaacataaa gaggaaggtg ccaccaaacg
     6121 cactaacttt agcctgaccg gtccgctggg cgacgaattc agcttccgct tgtatggcaa
     6181 cctcgacaaa acccaggctg acgcgtggga tatcaaccag ggtcatcagt ccgcgcgtgc
     6241 cggaacgtat gccacgacgt taccagccgg gcgcgaaggg gtgatcaata aagatattaa
     6301 tggcgtggtg cgctgggact tcgcaccatt gcaatcgctg gaactggaag cgggttacag
     6361 ccgccagggt aacctgtatg cgggcgatac ccagaacacc aactctgacg cttacactcg
     6421 ctcgaaatat ggcgatgaaa ccaaccgcct gtatcgccag aactactcgc tgacctggaa
     6481 cggtggctgg gataacggcg tgaccaccag caactgggtg cagtacgaac acacccgtaa
     6541 ctcgcgtatt ccggaaggtc tggcgggcgg taccgaaggg aaatttaacg aaaaagcgac
     6601 acaggatttc gtcgataacg atcttgatga cgtgatgctg catagcgaag ttaacctgcc
     6661 gattgatttc ctcgttaacc agacgctgac gctgggtacg gagtggaatc agcagcggat
     6721 gaaggactta agttccaaca cccaggcact gaccggaacg aataccggtg gcgctattga
     6781 tggcgtgagt gccaccgacc gtagcccgta ttcaaaagca gaaattttct cgctgtttgc
     6841 cgaaaacaac atggagctga ctgacagcac catcgtaacg ccggggctgc gtttcgatca
     6901 tcacagtatt gtcggcaata actggagccc ggcgctgaac atatcgcaag gtttaggcga
     6961 tgacttcacg ctgaaaatgg gcattgcccg cgcctataaa gcgccgagcc tgtaccagac
     7021 taacccgaac tacattctct acagtaaagg tcagggctgc tatgccagcg cgggcggctg
     7081 ctatctgcaa ggtaacgatg acctgaaagc agaaaccagc atcaacaaag agattggtct
     7141 ggagttcaaa cgcgacggct ggctggcggg catcacctgg ttccgtaacg attatcgcaa
     7201 taagattgaa gcaggctatg ttgctgtagg gcaaaacgca gtcggcaccg atctctatca
     7261 gtgggataac gtgccgaaag cggtggttga aggtctggaa ggatcgttaa acgtaccggt
     7321 tagcgaaacg gtgatgtgga ctaataacat cacttatatg ctgaagagtg aaaacaaaac
     7381 cacgggcgac cgtttgtcga tcatcccgga gtatacgttg aactcaacgc tgagctggca
     7441 ggcacgagaa gatttgtcga tgcaaacgac cttcacctgg tacggtaagc agcagccgaa
     7501 gaagtacaac tataaaggtc agccagcggt tggaccggaa accaaagaaa ttagtcctta
     7561 cagcattgtt gggctgagcg cgacgtggga tgtgacgaag aatgtcagtc tgaccggcgg
     7621 cgtggacaat ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga ccacgggcga
     7681 tttggcaggg gccaactata tcgccggtgc cggggcgtat acctataacg agccgggacg
     7741 tacgtggtat atgagcgtaa acactcactt ctgatgctaa cgtcagattg ttgacaaagt
     7801 gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa aatcttgcca
     7861 attcaatata ttgcaggacc atgtaggcct gataagcgta gcgcatcagg cagttttgcg
     7921 tttgtcatca gtctcgaata tggtcgatat gaaaactacg catacctccc tcccctttgc
     7981 cggacatacg ctgcattttg ttgagttcga tccggcgaat ttttgtgagc aggatttact
     8041 ctggctgccg cactacgcac aactgcaaca cgctggacgt aaacgtaaaa cagagcattt
     8101 agccggacgg atcgctgctg tttatgcctt gcgggaatat ggctataaat gtgtgcccgc
     8161 aatcggcgag ctacgccaac cggtgtggcc tgcggaggta tacggcagta ttagccactg
     8221 cggggctacg gcattggccg tggtttcccg tcaaccgatt ggcgttgata tcgaagaaat
     8281 tttttctgcg caaacagcga ccgaattgac agacaacatt attacaccag cagaacacga
     8341 gcgactcgca gactgcggtt tagccttttc tctggcgctg acactggcat tttccgccaa
     8401 agagagcgca tttaaggcaa gtgagatcca aactgatgca ggttttctgg actatcagat
     8461 aattagctgg aataaacagc aggtcatcat tcatcgtgag aatgagatgt ttgctgtgca
     8521 ctggcagata aaagaaaaaa tagtcataac tctgtgccaa cacgattaat tgacaacatc
     8581 tggtacgatt cgcccgcagc catcactgac tgcgggcgaa agtgtaaagc aggtgcctta
     8641 ccactctgac ctgacaaccg gatatgcggg gattgctccc cgcacaacgg ctacttcttc
     8701 ggttcgtaag cgagaacagc cttaaactca atattacgtt cctttacggt gaactcacac
     8761 agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac tatgactgcc
     8821 acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc
     8881 cgagttgttg gggctcggag tggcagcccc aggattgata gaaatatctc ctggggcttc
     8941 atctttccag acctcagagt taacctgaaa ccggaaagct tcaggcaccc gccgttatct
     9001 tacccttcct tagcaaaata gctgtttatt tatacagtga atcctgtttc ggacttctgt
     9061 cggcaaaact catcattgct cttaaaacga aatcgcccgc agccatcact gaccgcgggc
     9121 gaaagtgtaa agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact
     9181 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccctgaac tcaatattac
     9241 gttccttcac cgtaaactca cacagcgagt ctccgaccag aagcgtaaat cccggtaccg
     9301 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc
     9361 catgcgaaga aggaactacc atccaaattg tctggtttgg agtggcagcc ccagggttga
     9421 tagaaatatc tcctggagct tcatctttcc agacctcaga gtaaacctga aaccggaaag
     9481 cttcaggcac ccgccgttat cttacccttc ctcaacaaaa tagctgttta tttatacagt
     9541 aaatcctgtt tcggacttct cactcctcat ttaatcaact gaagctgagc ccgttttttc
     9601 tgcatgcctc aatcgtcaaa attcttcgcc accagccagc cgtgccataa cttgtcacat
     9661 tgcaaaagta gccaaattgc ttcagtcacc atcaccaaaa aaagaaacat tccagaactt
     9721 ccgcatctcg cccagccgct ccgacttcta tactgaatag aaaactccaa cataagagaa
     9781 acctatgcca ttacccgatt ttcatgtttc tgaacctttt accctcggta ttgaactgga
     9841 aatgcaggtg gttaatccgc cgggctatga cttaagccag gactcttcaa tgctgattga
     9901 cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg aaagtatgct
     9961 ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcaat tttcagcgat
    10021 gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg gcggtggcac
    10081 gcacccgttt cagaaatggc agcgtcagga ggtatgcgac aacgaacgct atcaacgaac
    10141 gctggaaaac tttggctatc tcatccagca ggcgaccgtt tttggtcagc atgtccatgt
    10201 tggctgtgcc agtggcgatg acgccattta tttgctgcac ggcttgtcac ggtttgtgcc
    10261 gcactttatc gccctttccg ccgcgtcgcc atatatgcag ggaacggata cgcgttttgc
    10321 ctcctcacga ccgaatattt tttccgcctt tcctgataat ggcccgatgc cgtgggtcag
    10381 taactggcaa caatttgaag ccctgtttcg ctgtctgagt tacaccacga tgatcgacag
    10441 cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg tggaggttcg
    10501 ggtgatggat accccgttaa cccttagcca cgcggtaaat atggcgggat taattcaggc
    10561 caccgcccac tggttactga cagaacgccc gttcaaacat aaggagaaag attacctgct
    10621 gtataaattc aaccgtttcc aggcctgccg ctatgggctg gaaggcgtca ttaccgatcc
    10681 gtacactggc gatcgtcgac cactaacgga agacaccttg cgattgctgg aaaaaatcgc
    10741 cccttctgca cataaaattg gtgcatcgag cgcgattgag gccctgcatc gccaggtcgt
    10801 cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggcg gctcgctgat
    10861 tgggctggtg aaaaagcatt gtgagatctg ggccggtgac taagccggga attgccttac
    10921 agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat gaaacaccct
    10981 ttagaaacct tgaccaccgc agcaggcatt ttgctgatgg ctttcctctc ttgcctgctg
    11041 ctgcccgccc ccgcactggg gcttacgctg gcacaaaaac tggtgaccac gttccatctg
    11101 atggatctta gtcagcttta cactttattg ttttgtctgt ggtttttagt gctgggcgct
    11161 attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc ggattaaacg
    11221 tcgattatcg ccgtacttcg cggcatactt tgcttattct ccttcgccta aaggaacgtt
    11281 tatggataag caatcactgc acgaaacggc gaaacgcctg gcccttgagt taccctttgt
    11341 cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg gcaagatttt
    11401 tatgctgtcg tcggagctac gcggcgtccc ctttatcaat ctgaagtccg atccacaaaa
    11461 atccctgtta aatcagcaaa tatacccaag cattaagcca gggtatcaca tgaataaaaa
    11521 gcactggatt tcggtgtatc ccggcgagga aatctccgaa gcgttacttc gcgatctgat
    11581 caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa aaagagtgcg
    11641 tccaggctaa agcggaaatc tatagcgcat ttttctcgct taccatttct cgttgaacct
    11701 tgtaatctgc tggcacgcaa aattactttc acatggagtc tttatggata tcatttctgt
    11761 cgccttaaag cgtcattcca ctaaggcatt tgatgccagc aaaaaactta ccccggaaca
    11821 ggccgagcag atcaaaactc tcctgcaata cagcccatcc agcaccaact cccagccgtg
    11881 gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat ccgctgccgg
    11941 taattatgtg ttcaacgaac gtaaaatact tgatgcctcg cacgtcgtgg tgttctgtgc
    12001 aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag atgctgatgg
    12061 ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt tcttcgccga
    12121 tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg tttatctcaa
    12181 cgtcggtaat ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac ccatcgaagg
    12241 ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct acaccagtct
    12301 ggtggtagtt ccggtgggtc atcacagcgt tgaagatttt aacgctacgc tgccgaaatc
    12361 tcgtctgccg caaaacatta ccttaaccga agtgtaattc tcccttgccg ggcatctgcc
    12421 cggctatttc ccctcagatt ctcctgattt gcataaccct gtttcagccg tcatcatagg
    12481 ctgctgttgt ataaaggaga cgttatgcag gatttaatat cccatgttga agatttagcg
    12541 ggtattgaga tcgatcacac cacctcgatg gtgatgattt tcggtattat ttttctgacc
    12601 gccgtcgtgg tgcatattat tttgcattgg gtggtactgc ggaccttcga aaaacgtgcc
    12661 atcgccagtt cacggctttg gttgcaaatc attacccaga ataaactctt ccaccgttta
    12721 gcttttaccc tgcaggggat tatcgtcaat attcaggcgg tattctggct gcaaaaaggc
    12781 accgaagcgg cagatattct gactacctgc gcgcagttgt ggatcatgat gtatgcgctg
    12841 ctttcagtct tctcgctgct ggatgttatt ttgaatctgg cgcagaaatt cccggcagca
    12901 tctcagttac cgctgaaagg gatatttcag gggattaaac tgatcggcgc gattctggtc
    12961 ggcattttga tgatctcgct gctgattggt cagtcgccag cgattctgat cagcggtctt
    13021 ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc cgattcttgg tctggtggca
    13081 ggtattcagc tttccgcgaa cgatatgctg aaactgggcg actggctgga gatgccgaaa
    13141 tacggcgcgg atggcgcggt gatcgatatt gggttaacca ccgtcaaggt gcgtaactgg
    13201 gacaatacca ttaccactat tcccacctgg tctctggttt ctgactcctt taaaaactgg
    13261 agcgggatgt cagcatctgg cgggcgacgt attaagcgca gtatcagtat tgatgtcacc
    13321 agtattcgtt ttcttgatga agacgaaatg caacgtctga ataaagcgca tttgttaaag
    13381 ccttatttaa ccagccgcca tcaggaaatt aatgagtgga atcgccagca aggttctacg
    13441 gagtcggtat taaacctgcg ccgaatgacc aatattggaa cctttcgtgc ctatctgaac
    13501 gaatatctgc gtaaccatcc gcggattcgt aaagatatga ccttaatggt acgccaactg
    13561 gctccaggtg ataacggttt accgctcgag atctatgcgt ttaccaacac cgtggtgtgg
    13621 ctggaatatg aaagcattca ggctgatata ttcgatcaca tatttgcgat tgtcgaagag
    13681 tttggtctgc gacttcatca gtcgccaacc ggcaatgata ttcgctctct ggcgggtgca
    13741 tttaagcagt aattaaaaaa accgctctca tcgaatggat gagagcggac atcgttttgt
    13801 cggatgtagc gtgaatgcct tatttccgac gcagcgtttt aaatgctaca aacaggaata
    13861 caatccacac cggcagcagg atcgctgaca agcgcatatc atccatcgtg cacatcagca
    13921 gcaaaatcat ggcgaggaag gcgatgcaaa gatagttgcc gaacggatag agcagcgcct
    13981 taaactgtgt ttcacgcccc tgacgtcgca tcgctgcacg aaaacgcaga tgcgccagac
    14041 agatcataat ccagttcaac agcagcgttg ctaccaccag cgccatcagc agaccaaacg
    14101 ctttttgcgg cagcagatag ttgattaaca ccaccagcga agtgatcgct ccggaaagca
    14161 tcagcgagtt aatcggcacg ccgcgacggc taacacgggt aaggaacttc ggcgcattac
    14221 cctgcacaga aaggccaaac agcatgcggc tgttagagta aaccccgctg ttatacactg
    14281 acagcgatgc taccagaatg acgaagttca gcgcagaagc taccacgttg ctgtcgagat
    14341 tatggaaaat catcacaaac gggctactgt tggatttcac ttccacccac ggatagagcg
    14401 ccagtaaaac caccagtgaa ccgatgtaaa acagcaggat gcgatacacc acctgattta
    14461 ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc ggcagtaatc ccaatcagct
    14521 ccagaccgcc gaaggagaac ataattaccg ccagcgacaa aatcagccca ttccagccgg
    14581 tggcgaagaa accaccgtag cgccagaggt tgtcgatact ggctttctcg ccgccgtgac
    14641 cagaaaacag cagccacagg ccaaagccga tcataccgat gattgccagc actttaatca
    14701 gcgcaaacca gaactcggtt tcgccatata agcgcacgtt caccaggtta acggcgttga
    14761 tgataataaa gaaggcggca gcccaaatcc acgttggaac atccgggaac cagtactgca
    14821 tatagatgcc cgcagcggtc agctctgcca ttcccaccag cacgaacatc acccagtagt
    14881 tccagccgga gaggaacccg gcgaacggtc cccagtattt ataggcaaag tgggcaaatg
    14941 aaccggatac cggctcttcg accaccatct cgccgagctg gcgcataatc aggaaagcga
    15001 tgatcccggc gacgccgtag cccagcaata cagccggacc cgccatctga atcgccgggc
    15061 caatgccaag aaacagacca gtaccaattg cgccacccag cgcaatcagt tgaatatgac
    15121 ggttatgtaa tccgcgatga agcgtcggct cttgattcga cgcagtatct tccgatacgt
    15181 ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt gttgaggggc accttttaac
    15241 atttagtgcc catcgtcgca agacacaatc cacacggtta aaccgggtat cctgctttta
    15301 tttccgtacc cgatgtcggt gcagccacat cagcttatac gctgccggga taataaacag
    15361 cgacagcaaa ggcgcggtga tcatgccgcc aatcatcggc gcggcaatcc ggctcatcac
    15421 ctctgatcct gcccccgttc cccacagaat cggcagcaga ccagcgataa tcaccgccac
    15481 cgtcatcgct ttcgggcgca cgcgcaggac cgcgccgtga tgtaacgcct catccagctt
    15541 ctgctcgctg aatgtttgcg gattattcaa cgacggctcg gcctctatgg cgtgacgtaa
    15601 atacatcagc atcaccacgc caaattcggc ggcgaccccg gcaagggcaa taaaaccggt
    15661 gcccgtcgcc acagaaagat gaaagcccat ccaccacagc aaccagatgc cgcccaccag
    15721 cgcaaacggt acgctgctga taatcagcaa cgcttcgccc acccgacgga acgccagata
    15781 caacagcacg aagatgatca tcaacgtcat cggcaccatg agtttcagct tatggttggc
    15841 gcgctccagt agctcgaact gcccggagaa tgccacgctg gtgcccggtt ttaactgcac
    15901 tttttcagct atcgcttttt gcaaatcgtg aaccaccgac accatgtcac gatcgcgggc
    15961 atcgatataa atccagctcg tcgggcgcgc attctcggtt ttcagcatcg acggtccggt
    16021 agagacttta acgtcggcca cgtctgccag ggtgatttgc tgcttcatcg gcgtcaggat
    16081 cggcaactgg cgcagtgcct gcggactatc gcgccagctt tgcggataac gcagattaat
    16141 tggataacgg gcaatccctt ccaccgtttc gccaaccatc gccccgccca ccgcagaagt
    16201 cacaaacaac tgcacatccg ccaccgtcat accgtaacgt gcggcttttt cacggttaat
    16261 ctcaacgttg atatagcgcc caccttccag ccgctcggca agcgcagaag ccacgcctgg
    16321 caccgttcgt gccacctctt caatttgctc cgccatcgcg tcgatatccg ccagcacagt
    16381 gccggaaact tttatgccga tagggctttt aatgccggtt gagagcatat cgatacggtt
    16441 acgaattggc ggcacccaca gattcgccag ccccggcaga cgcacggtgt tatccagttc
    16501 ctcaatgatt ttgtccattg tcatgcctgg ccgccactgc tcctgcggct taagctggat
    16561 ggtcgtttct accatctcca gcggagcaga atcggtggcg gtttccgctt tcccggtttt
    16621 gccaaatacc cgcgccactt caggtacgct cataattagc ttgtcggttt tttgcagcat
    16681 actcgccgcc tctgctgcgg aaatccccgg cagcgtcgat ggcatataca acaagtcgcc
    16741 ttcattgatc tgcggtaaaa attccccgcc aactttattg agcggccaga gaaccgtcag
    16801 caccgaaagc gctgccacca acagcgtggt tttcggccag tgcagtactt tcagcaacag
    16861 cggatgataa acacgaatca aaaagcgatt gagcgggtta ctgctttccg gcggaatttt
    16921 gccacggatc cagtaaccca tcaggatagg gatcactacg atcgccagca gcgccgcacc
    16981 cgccatcgca tacgttttgg tgaacgccag cgggccaaac agacgccctt cctgcccttc
    17041 cagggtgaag atcgggataa acgacagcgt gataatcagc agactgataa acagcgccgg
    17101 ccccacttca acagatgcat tggtaatcac ctgccagcgc gttttattat ccagcgtggc
    17161 gtcaggatgc tggtgctgcc actcttccag ccgtttatgt gcattctcga tcatgacgat
    17221 agcggcatcg accatcgccc cgacggcaat cgcaatgcca cccaacgaca taatattggc
    17281 attcagcccc tggaagtgca tgacaataaa agcaatacac aaccccagcg gcaacgaaat
    17341 aatcgccacc agcgccgagc gcacatgcca gagaaacagc gcacagacta ccgccaccac
    17401 aataaactct tccagcaact tgccgctgag gttgtcgatg gcccggtcaa tgagctggct
    17461 gcgatcgtat gttgtaacta tctccacgcc ttccggcaga ctgcttttca gcgtttccag
    17521 tttgtccttc acggcggcga tcacttcacg ggcgtttttg ccggagcgta ggatcaccac
    17581 cccgcccaca acttcgccgt tcagttcggc aatgccccgg cgcatctccg ggccaacctg
    17641 gatcttcgca acatcacgca gataaacagg cacgccattt tcactggctt ttaaaacgat
    17701 gtgattaaag tcgtcgagcg tttgtagata gccgctggcg cgcaccatat attctgcttc
    17761 cgccagttcg attgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac
    17821 ttcggcgaga ctgatgccat actgcgccag gcgctgagga tcgataacca cctgatactc
    17881 tttcaccacg ccgcccaccg acgccacttc cgcaacgtca gggatggttt ttagctcata
    17941 tttgagaaac cagtcctgca atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc
    18001 caccagtgca tattcataga tccagccaac acccgtggca tctggtccca gctcggcgct
    18061 gactcccgca ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc
    18121 ccagtacgga tcggtaccat cttcgaaaat gacatacaca taagagtcgc caaactgcga
    18181 gaaaccacgc acagtcttcg cgccaggcac cgacaacatg gtggtagtta gcggataagt
    18241 cacctgattt tcaacgattt gcggtgcctg accgggatag ctggttttaa taatcacctg
    18301 cacatcggag agatccggca acgcatccac tggcgtgtta atgatggtcc aggtgcccca
    18361 gatactcaga aataacgcgc ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat
    18421 aatccattca atcattggtt attccctcaa tgcgcatggg tagcactttc agagcgcatc
    18481 cgctccagtg cgccagaaat attggcttcg gaatcaatca ggaacaggcc gctggaaacc
    18541 accttttcac cttccgccag accagagcgt aatgcggtga caccttgcga tgcctggaaa
    18601 acggcaacgc gttttggtac aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg
    18661 ctgccggtat caatcagcgc ctgtgacgga atgagcagca tcggttcgct ggcagtattg
    18721 agttgcaacc aggcgttcat tcccggtttt agcgcctcgt cggcgttgtc gacttccaga
    18781 cgcagctgca gcgtgcgggt cgcggcatcc acgccaggta gtagcgtcca tttgcggatg
    18841 gtgagtgttt tatcaggtcg cgccggaacg gtgagcgtaa actgcgaggc atctttcacc
    18901 agccaggcga tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt
    18961 ttcgctacca cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc
    19021 acgccatcaa tgggtgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg
    19081 cggcgaatat ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcggtc
    19141 tgggtcgccg taccgccggt ttcgcgcagc agtaaatact cactctgcgc ttccacccag
    19201 tcgggaatgg tcaggtcgag aagcggcgcg cccttttgta ctttatcgcc cacggtaagc
    19261 ggatacacct tgtcgataaa cccggcggcg cgagcctgca caatggcata ctgatactcg
    19321 ttgtaactga cattcgccgg gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta
    19381 gccgttttca cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa
    19441 ctctcttcat cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt
    19501 ttatcgaacc gcgtattggg atacatcggg tcgtaccaga ataagatttt acgttctgcg
    19561 gtcgacgttt tttctgcagg cggttccgcc tttgcaaacc aggtaaaacc tgccgcagaa
    19621 ataataccgc ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac
    19681 ctgggttact ggctgacttt aatatcctgt aataaagaaa ggttgccctg ctggacaaaa
    19741 ttaaacgcca ctttgtcgcc ggttttaatt ccactcattt tcgtctgcgg ggtgatggta
    19801 aagcgcatgg tcatctccgg ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt
    19861 tttttgcttt ccagatcaaa accttttacc acgccggtgg cactaatgac ctgtggttgt
    19921 gcttcgctca tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta
    19981 aacagactga acattgcgac ttgcagtgct tttttcattt attttctcct ggagttaaaa
    20041 attattgctg ccaaccgcca ccaagtgcgg tatataaaga aatttcgttg acctgacggg
    20101 cataattcag gtcaagtacg gtttgtcggg ttgcaaataa agaacgctcg gcatccagca
    20161 cttccagata acttactgcg ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt
    20221 gcagcgatgc cagataacgc tgctgggcgc taatttgatc gttcaggctt tgacgtaatg
    20281 ccagcgcatc tgccacttct ttaaaggcgt tctggatttt ctgttcataa ttcactaccg
    20341 actgctgctg gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa
    20401 tggggatctc aattttagga ataaaattcc acatcccgct gctggcgtta aataatgacg
    20461 acagatcgct gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg
    20521 cgcgtgcagc accaatattg gcattagccg ccattaacgc atgttcagct tccataatat
    20581 ccgggcgctg caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc
    20641 tgtcgctgtt tactgtctgc gcttgcggca gcttgccgta gcttcccagt aacagttgca
    20701 atgcattatt cgcctgcgcc agttccccct gacgtttagc gatgtcgctg cgggtacttt
    20761 caatcacccc gcgagcctgt tccagcgcca gaacattact gctacctgtc aacagttgtt
    20821 tttcgacaaa cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt
    20881 gcgcatacgc cagctgctga ttgaaatagc tttgcgcgac attagaaacc agcagaatat
    20941 gcaccgcgcg ctgagcttcc tcagtggcta aataattttg tcgctcggct tcgctcatgt
    21001 tctttaagcg cccgaaaaaa tcgaggtcaa agctggcgtt aaggccagta gaaaactccc
    21061 gcgtcgtggc tgaatcgcct ttaagattgc cgctccagct gccgctgccc tcaccattga
    21121 gctgtgggta gcggtcggca tcagtcagac gatattgcgc ccgtgcctcc tgcactttca
    21181 gcgtcgccat gcgcaaatcc cggttattct ccagcgcctc gctaatcagc gtcttcacct
    21241 gattatcaac aaaaaaggtg cgccagcccg cgttctgata gttatctgct gcgttaacca
    21301 ggccgttctg gctgagtgag aactgctgcg gtacgggcat tgccgggcgc tgataatccg
    21361 gtgccagtga acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag
    21421 acataggctc ataatttctg gtgattttat accgccaact ttactcgcca ggctctgatt
    21481 ttccggtgac aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc
    21541 ccgctactct agaattgccc gggcaacatg cggaggaaat atgaaactgt tgattgtcga
    21601 agatgaaaag aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt
    21661 cgatttggcc gacaacgggc tgaatggcta ccatctggcg atgaccggag attatgacct
    21721 gataatcctc gatattatgc tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg
    21781 ctccgccaat aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg
    21841 cgtcaagggg ctggagttgg gggccgatga ctatctggtg aagccgttcg cgtttgctga
    21901 actgctggcg cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca
    21961 gtttcaggtt gccgatttga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac
    22021 gcgcatcact ctgaccagta aagagtttac tctgctggag ttttttctcc gccatcaggg
    22081 cgaagtgctg ccccgctcgc ttatcgcctc gcaggtatgg gacatgaatt ttgacagcga
    22141 cactaacgcc attgatgtgg cggtgaagcg gctacgcggc aaaatcgaca acgactttga
    22201 gccgaagctg attcaaaccg tgcgcggcgt gggttacatg cttgaggtgc cggatggtca
    22261 gtaagccatt tcagcgcccg ttttcgctgg caacccgcct gacctttttt atcagcctgg
    22321 ccaccatcgc ggcgtttttc gcctttgcat ggatcatgat ccactcagta aaagtgcatt
    22381 ttgccgagca ggatattaat gatttaaaag agattagtgc cacccttgaa cgcgtactaa
    22441 atcaccctga cgaaacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt
    22501 attccaatgt gttgatttcc ctggcagata gccacggtaa aacggtgtat cactcccccg
    22561 gtgcgcctga tatccgcgag tttgcgcgcg atgccatacc cgataaagac gcgcggggcg
    22621 gcgaggtgtt tctcctctcc ggcccgacga tgatgatgcc aggccacggt cacgggcata
    22681 tggaacacag caactggcgg atgatcagtt tgccagttgg cccactggtt gacggcaaac
    22741 cgatttatac gctctacatc gcgctttcga tcgattttca tcttcattac ataaatgatt
    22801 tgatgaataa acttattatg accgcatcgg tcatcagcat cctgatcgtc tttatcgtgc
    22861 tattggcggt tcataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata
    22921 tcacctcgaa agatctcgac gtccgccttg acccgcagac cgtgccgatt gagctggaac
    22981 agctggtact gtcgttcaac catatgatcg agcgtattga ggatgtcttt acccgccagt
    23041 ccaatttctc agcggatatc gcccacgaaa ttcgcacacc aattactaat ctcataacgc
    23101 aaacggaaat cgccctcagc cagtcgcgca gccagaagga gctggaagat gtgctctact
    23161 ctaatctcga agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgccc
    23221 aggccgataa caaccagtta atccccgaaa agaaaatgct caacctggcg gatgaagtcg
    23281 gcaaagtgtt cgattttttc gaggctttag cggaagatcg cggcgtggag ctgcagtttg
    23341 ttggcgacga gtgccaggtt gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc
    23401 tgctttcgaa cgccctgcgt tatacgccac ccggagaggc tattgtagtg cgctgccaga
    23461 cggtcgatca tctggtgcaa gttatcgtcg aaaaccccgg tacgcccatt gcgcccgagc
    23521 acttaccgcg attgtttgac cgtttctacc gcgttgaccc ctcccgccaa cgaaaaggcg
    23581 aaggtagcgg cattgggctg gcgatagtga aatcaattgt tgtcgcgcat aaaggcacgg
    23641 ttgcggttac gtcaaatgcg cgggggacaa ggtttgtgat tgtgttgccg gagagggggt
    23701 gattattaag ttcaaattca gggcgagggt ttacggcgtt tttcgctaaa tttccatcat
    23761 tataaacaag ccacctatca tataaagcaa tgaataatta aatcgcgatt aatcatcatt
    23821 aatttttata ttattcaccc aatccattta taaagtgaat ttgataaatt cctaatttaa
    23881 aatatccact attgagccag tatttaacaa cgttaagatg tgcaatgtgc agatcatgtt
    23941 atcagggtgg agcggtagca tatatttttt aagcaatcat tcattttaat gatgttaatt
    24001 gggttattta gttgtgaata aaaacatatt atgaatattg gtttaacttt gcgtttttgt
    24061 tactcgctgt gatgtgcgca cctgtcctga ataacgtcag gaaacaataa gttgagcttg
    24121 tgattatttt tttgactgaa cagacacccg tttctgtaaa gtaatattta aacaaaatcc
    24181 tcacataatc atagcatttt ttgttccttc cctaataacg tcttaagtcg tcccgcttat
    24241 ctcattatcc gcagttgtac ggtgcggatt attattcatt ttaccggagg ttgctatgtc
    24301 aaccggatta cgtttcacac tggaagtgga cggcctgcca ccggacgctt ttgcggtggt
    24361 ttcctttcat ctcacccagt cactctcttc gctcttttcc ctcgatctct ccttggtcag
    24421 ccagcagttt ctctcccttg aatttgcgca ggtgctggac aaaatggcct acctgacaat
    24481 atggcagggt gatgatgtac agcgccgggt gaaaggtgtg gtgacctggt ttgaactggg
    24541 ggagaacgac aaaaaccaga tgctgtacag catgaaggtg cacccgccgc tgtggcgtgc
    24601 cggtctgcgc cagaacttcc gtatcttcca gaacgaggac atcaaaagca tcctcggcac
    24661 gatattgcag gaaaacgggg tgaccgagtg gagtccgctg ttcagtgaac cgcatccttc
    24721 ccgtgagttt tgtgtccagt acggtgagac tgattacgat ttcctgtgcc ggatggcggc
    24781 ggaggaaggc atcttctttt atgaggagca tgcttacaaa agtaccgacc agagcctggt
    24841 gctgtgcgac acggtccgcc atctgcccga atcttttgaa atcccctgga acccgaacac
    24901 ccgtaccgag gtgagcaccc tctgcatcag ccagtttcgc tacagcgcac aaatccgccc
    24961 ttcttccgtg gtgaccaaag actacacctt taaacgcccc ggctgggcag gacgttttga
    25021 tcaggaaggc cagcaccagg attaccagcg cacgcagtat gaggtgtatg actaccccgg
    25081 acgtttcaag ggcgcccacg ggcagaactt tgcccgctgg cagatggagg gctggcgaaa
    25141 caatgcagaa accgcgcggg gaatgagtcg ctcgccggag atatggccgg gacggcgaat
    25201 tgtgctgacg gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga
    25261 actgcacggc gagcagccgc aggcggtgcc gggaaggcgg ggagcaggga cggcgctgga
    25321 gaaccatttt gcggtgatcc cggcagaccg gacatggcga ccccagccgc ggctaaaacc
    25381 gctggtggac ggtccgcaga gcgccgtcgt gacggggccg gagggtgagg aaatcttctg
    25441 tgatgaacat ggccgagtgc gggtgaaatt caactgggac cgttataacc cggcagacca
    25501 ggacagctcg tgctggatcc gtgtggcaca ggcgtgggca ggcaccggtt ttggcaacct
    25561 ggcgataccg cgtgtgggtc aggaggtgat agtggacttc ctcaacggcg atccggacca
    25621 gccgatcatt atggggcgca cctaccacca ggaaaaccgc acccccggca gcctgccggg
    25681 gacgaagacg cagatgacca tccgttccaa aacgtatatg ggcagcgggt ttaatgagct
    25741 gaagtttgat gatgcgacag ggagagaaca ggtctacatc cacgcgcaga agaacatgga
    25801 taccgaagtg ctcaacgacc gtaccaccac cgtaaaacac gatcatcgcg aaaccgtaaa
    25861 aaatgaccag acggtcacga tccaggaagg taaccgcctt cttacggtgg aaaaaggcca
    25921 caagatcacc ggagtactga aagggtcttt atctgaggat gtctttcagg acagaggcac
    25981 gattgccggt tcggtgcatg ttgacgctgt aaacaatggt ggcgaaggca acggtataca
    26041 ggcttatacg gcaattaagg aaattatgct ggccgtggag gaaagcaaaa ttgcgctgac
    26101 gccggatggc attcagctac aggtcgggga atcaacggta atcaggctgt cgaaggatgg
    26161 catcaccatc gtgggcggtt ctgttttcat caactgattc actcagatta acagggagta
    26221 ttttatgcag ttcactttta atgaaggtca tattcaactg ccatcgcaat ggcaggatca
    26281 gtcgatgcag gtcctggtat ccacggataa cagtggcatc aacctggtca ttacccgcga
    26341 acccgtttca cagggaaccc tgacaccaga gctgtaccag gaaacgctgg cgctgtacca
    26401 gggcaaactt gatgggtaca ccgaacatgc ctgccgggaa atcactctgg cagaggcccc
    26461 ggcctggctg ctggattatt cctggaacgg tccggaagat gaagggaatc aggggcggat
    26521 tagccagata gccgtattcc agcggcgtgg agatacgctg ctgaccttca ctttcagcac
    26581 ctccttatcc ctgaagaaca gtcaaaagac gatgctgctg gaggtgataa agagcttcac
    26641 cccgttgcca ccggagaatg acatccaaaa ggaccagccg cgctgacata aacgagggat
    26701 aaaacatgag tgaaggacca ggcgggccac agggagcgac cgcaggcggt acgctggcaa
    26761 tgcggatgct gtcgcagcgg gcgatggccg ccagccagat gaaacgggca gccaacgaca
    26821 aagccattgc acagatgttg gcatcaaaaa agtctggccc ccccgccgcc aggctgggcg
    26881 atgaaattca gcacaagagt tttttggggg cgctggcagg ggccgtgctg ggggcgatag
    26941 tgaccatagc agaaggttgc ctgattatgg ccgcctgtgc caccggccct tatgcgctgg
    27001 ttctggtgcc tgcgctgatg tatgccagct ataaggcgag tgattatgtg gaggagaaac
    27061 agaaccagct tgaatcatgg ataaacagct tttgtgacac ggacggcgcc atcaataccg
    27121 gttctaaaaa tgtaaaaatt aacggaaagc cagcagcccg tgcagccgtc acccttcccc
    27181 ctcctccccc acctggagca atacctgaag tcccacaggg ggaaccctca tggggtgata
    27241 ttgccactga cctgcttgaa tcggcagcgg aaaaagcagt accactggcg aaggcctggg
    27301 ggaacgctgt tatcaccctg acggacagca atgccggttt tatggatcgc gtcagcgccg
    27361 gcgcatcgct tctgtttccc gccggtccgg tattaatgga gtttgccacc atggtgggcg
    27421 ggcgtggcga aatcaaaaaa gatgtggatt tcccggaagc cggtgaggac acggcgctct
    27481 gcgacaagga gaacaaacca ccgaggatag cccagggcag cagcaacgtc tttatcaaca
    27541 atcagcctgc cgcgcgcaag ggcgacaaac tggagtgcag cgcggcaatc gtggaaggtt
    27601 cgccggacgt ctttattggg ggtgagcagg tcacctatct ggatatccag ccggagttcc
    27661 cgccatggca gagaatgatc ctgggaggaa taacgatagc cagctatctt ctgccgccag
    27721 caggactgct gggaaaactg gggaatctgg cgaaactggg caaactggga aacctgctgg
    27781 ggaaaagcgg taagctgctg ggcgcaaagc tcggcgcgtt gctggggaaa acaggtaatt
    27841 cgttaaaaag tattgccaat aaagtcatca gatgggtaac agatcctgtc gatccggtaa
    27901 ccggcgcata ctgcgacgaa cgtaccgact ttacgctggg ccagacactc cccctctcct
    27961 tcacccgttt ccacagttct gtactgccgc tgcatggcct gacgggcgtg ggctggagcg
    28021 actcctggag cgaatacgcc tgggtgcgtg aacagggaaa ccgggtggat gtcatcagcc
    28081 tgggagccac gctgaacttc gccttcgacg gtgaaagtga tacggcggtt aacccgtatc
    28141 acgcccagta cattctgcgc cgccgtgatg attatctgga gctgttcgac agggatgcac
    28201 tgagcagccg cttcttttat gacgcctttc cgggaatgcg tctgcgccac ccggtgactg
    28261 acgataccag cgatgaccgc ctggcacaca gccccgcaga ccggatgtac atgctgggcg
    28321 ggatgagcga caccgccagc aaccgcatca cgtttgagcg cgacacccag taccggatca
    28381 cgggtgtcag tcacaccgac gggatccggc ttaaactgac gtaccacgcc agcggctacc
    28441 tgaaagccat tcaccgcacg gataacggca tacagacgct ggcgacctac gaacaggatg
    28501 cgcggctgga ctaccacctg ttttatgagt acgacgctgc ggaccggatc atccgctggt
    28561 ccgataacga ccagacgtgg agccgtttca cctacgatgc acagggccgg tgcgtgaccg
    28621 tcaccggggc ggagggctat tacaacgcca cgctggacta tggtgacggc tgcaccaccg
    28681 tgacggacgg caagggcatt caccgttatt actatgatcc tgacggcaat attctgcggg
    28741 aagaagcgcc ggacggcagc accaccacgt atgaatggga tgaattccat cacctgctgg
    28801 cccgccactc ccctgccggg cgggtggaga agtttgaata caacgccgca cacggtcagt
    28861 taagccgtta cacggcggca gacggcgcgg attggcagta ctgctatgat gagcgcggcc
    28921 tgctcagcaa catcaccgcc cctgccgggc agacgtggac gcagcagtgt gatgaacgcg
    28981 gcctgccggt gagtctggta tcgccacagg gcgaagagac ccggctggcg tacaccgctc
    29041 aggggctgct gtcggggata ttccgccagg atgaacggcg tctgggcata gagtacgacc
    29101 accacaactg gccggaaaca ctcaccgacg tgatgggccg cgaacaccac accgaataca
    29161 gcggtcacga cctgccggtg aagatgcgcg gccccggcgg tcagtcagtg cggttgcagt
    29221 ggcagcagca ccataaactg agtggccttg agcgggcagg aaccggcgcg gaaggattcc
    29281 gctacgaccg ccacggcaac ctgctggcgt acacggacgg taacggcgtt gtctggacaa
    29341 tggagtacgg cccgtttgat ttgccggtgg cgcgaacgga cggtgaaggc caccgctggc
    29401 agtaccgcta cgataaagac acgctgcaac tgacagaagt cattaacccg cagggcgagt
    29461 cttatcttta tattctggac aactgtggcc gggtgacgga agaacgtgac tggggcggcg
    29521 tggtctgtcg ttaccgttat gacgctgatg gcctgtgtac cgccagggtc aacggcctgg
    29581 aggaaaccat cctctacagc cgggatgccg caggccgcct ggcagaagtc atcactccgg
    29641 aaggcaaaac gcagtatgcg tatgacaaat ccggcaggct gacgggtatc ttcagcccgg
    29701 acggcacatc acagcgcacc ggctatgatg aacgcgggcg ggtgaatgtc accactcagg
    29761 gccgacgggc cattgaatac cactaccccg acgaacacac cgtcatccgc tgtatcctgc
    29821 caccggaaga tgaacgcgac agacaccccg acggatccct gctgaaaacc acataccgct
    29881 acaacgccgc cggagaactg acggaggtta tcctgccggg ggatgagacg ctgacgttca
    29941 gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa ccggggtttt gcctgtgaac
    30001 agggctggaa tgcagccggt cagcctgtca gccagcgcgc cggacttttc ccggcggaag
    30061 ccacatgggg cggactgctc ccttcactgc tacgggaata ccgttacgac agcgcgggta
    30121 acgtatcagg cgtcaccagc cgggaagatt acggacggga aacacaccgg gagtaccggc
    30181 ttgaccggaa cggccaggtc acggcggtga cagcctcagg caccgggctg ggctatggcg
    30241 aaggcgatga gtcctatggc tatgacagct gtggctacct gaaggcgcag tctgcgggcg
    30301 ggcaccggat aagtgaagag actgaccagt atgccggagg ccaccggctg aaacaggccg
    30361 gaaacacgca gtatgactat gacgccgcag gccggatggt cagccgcacc agacaccgtg
    30421 acggctatcg cccggaaaca gagcggttcc ggtgggacag ccgggaccag ctgaccgggt
    30481 attgcagcgc acagggtgag cagtgggaat accgccacga cgccagcggc agacgaacag
    30541 agaaacgctg cgaccggaag aaaatccgct tcacgtacct gtgggacggc gacagtattg
    30601 cggaaatccg ggaataccgc
//
LOCUS       DAZGXS010000046        30196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGXS010000046 DAZGXS010000000
VERSION     DAZGXS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(376..2205)
                     /gene="glmS"
                     /locus_tag="ACHKG5_002195"
     CDS             complement(376..2205)
                     /gene="glmS"
                     /locus_tag="ACHKG5_002195"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0868299.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2367..3737)
                     /gene="glmU"
                     /locus_tag="ACHKG5_002196"
     CDS             complement(2367..3737)
                     /gene="glmU"
                     /locus_tag="ACHKG5_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0868300.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(4089..4508)
                     /gene="atpC"
                     /locus_tag="ACHKG5_002197"
     CDS             complement(4089..4508)
                     /gene="atpC"
                     /locus_tag="ACHKG5_002197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0868301.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4529..5911)
                     /gene="atpD"
                     /locus_tag="ACHKG5_002198"
     CDS             complement(4529..5911)
                     /gene="atpD"
                     /locus_tag="ACHKG5_002198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0868302.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5938..6801)
                     /gene="atpG"
                     /locus_tag="ACHKG5_002199"
     CDS             complement(5938..6801)
                     /gene="atpG"
                     /locus_tag="ACHKG5_002199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0868303.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6852..8393)
                     /gene="atpA"
                     /locus_tag="ACHKG5_002200"
     CDS             complement(6852..8393)
                     /gene="atpA"
                     /locus_tag="ACHKG5_002200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0868304.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8406..8939)
                     /gene="atpH"
                     /locus_tag="ACHKG5_002201"
     CDS             complement(8406..8939)
                     /gene="atpH"
                     /locus_tag="ACHKG5_002201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0868305.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8954..9424)
                     /gene="atpF"
                     /locus_tag="ACHKG5_002202"
     CDS             complement(8954..9424)
                     /gene="atpF"
                     /locus_tag="ACHKG5_002202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0868306.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDVVDKLVAEL"
     gene            complement(9486..9725)
                     /gene="atpE"
                     /locus_tag="ACHKG5_002203"
     CDS             complement(9486..9725)
                     /gene="atpE"
                     /locus_tag="ACHKG5_002203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0868307.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9772..10587)
                     /gene="atpB"
                     /locus_tag="ACHKG5_002204"
     CDS             complement(9772..10587)
                     /gene="atpB"
                     /locus_tag="ACHKG5_002204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0868308.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10596..10976)
                     /gene="atpI"
                     /locus_tag="ACHKG5_002205"
     CDS             complement(10596..10976)
                     /gene="atpI"
                     /locus_tag="ACHKG5_002205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0868309.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11593..12216)
                     /gene="rsmG"
                     /locus_tag="ACHKG5_002206"
     CDS             complement(11593..12216)
                     /gene="rsmG"
                     /locus_tag="ACHKG5_002206"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0868310.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12280..14169)
                     /gene="mnmG"
                     /locus_tag="ACHKG5_002207"
     CDS             complement(12280..14169)
                     /gene="mnmG"
                     /locus_tag="ACHKG5_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0868311.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14548..14991)
                     /gene="mioC"
                     /locus_tag="ACHKG5_002208"
     CDS             complement(14548..14991)
                     /gene="mioC"
                     /locus_tag="ACHKG5_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0868312.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(15081..15539)
                     /gene="asnC"
                     /locus_tag="ACHKG5_002209"
     CDS             complement(15081..15539)
                     /gene="asnC"
                     /locus_tag="ACHKG5_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0868313.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15691..16683
                     /gene="asnA"
                     /locus_tag="ACHKG5_002210"
     CDS             15691..16683
                     /gene="asnA"
                     /locus_tag="ACHKG5_002210"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0868314.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16688..18139)
                     /gene="viaA"
                     /locus_tag="ACHKG5_002211"
     CDS             complement(16688..18139)
                     /gene="viaA"
                     /locus_tag="ACHKG5_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP0868315.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(18133..19629)
                     /gene="ravA"
                     /locus_tag="ACHKG5_002212"
     CDS             complement(18133..19629)
                     /gene="ravA"
                     /locus_tag="ACHKG5_002212"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0868316.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19852..21720
                     /gene="kup"
                     /locus_tag="ACHKG5_002213"
     CDS             19852..21720
                     /gene="kup"
                     /locus_tag="ACHKG5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0868317.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21887..22306
                     /gene="rbsD"
                     /locus_tag="ACHKG5_002214"
     CDS             21887..22306
                     /gene="rbsD"
                     /locus_tag="ACHKG5_002214"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0868318.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22314..23819
                     /gene="rbsA"
                     /locus_tag="ACHKG5_002215"
     CDS             22314..23819
                     /gene="rbsA"
                     /locus_tag="ACHKG5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP0868319.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23824..24789
                     /gene="rbsC"
                     /locus_tag="ACHKG5_002216"
     CDS             23824..24789
                     /gene="rbsC"
                     /locus_tag="ACHKG5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0868320.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24814..25704
                     /gene="rbsB"
                     /locus_tag="ACHKG5_002217"
     CDS             24814..25704
                     /gene="rbsB"
                     /locus_tag="ACHKG5_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0868321.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25830..26759
                     /gene="rbsK"
                     /locus_tag="ACHKG5_002218"
     CDS             25830..26759
                     /gene="rbsK"
                     /locus_tag="ACHKG5_002218"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0868322.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26763..27755
                     /gene="rbsR"
                     /locus_tag="ACHKG5_002219"
     CDS             26763..27755
                     /gene="rbsR"
                     /locus_tag="ACHKG5_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0868323.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27721..29148)
                     /gene="mdtD"
                     /locus_tag="ACHKG5_002220"
     CDS             complement(27721..29148)
                     /gene="mdtD"
                     /locus_tag="ACHKG5_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0868324.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(29171..29863)
                     /gene="yieP"
                     /locus_tag="ACHKG5_002221"
     CDS             complement(29171..29863)
                     /gene="yieP"
                     /locus_tag="ACHKG5_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0868325.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7310 a         7710 c         7687 g         7489 t
ORIGIN      
        1 tagaaaacag tcttattttt agtaaaccac actttcaaat tatatttttt gcctatattt
       61 gtggaaagac tatacattta cagtgataat ttattatcac acattgttac atagttaaat
      121 aaaattacat attgatgatt gttttacaag cgattcttga atgaacaaaa cgtgctattc
      181 attacaaagt acatcaatta tggcaattgt ttgtcaatga ttcgaggtat ttaattacta
      241 ctacggttaa aacaagtgtt caacggtgcc ccaaataata gctgaagtta attatatgga
      301 atgaggttag attattaatg ctggtaagta aaagggccgc caaaaggcag cccttcgttc
      361 agcaaatgaa taatattact caaccgttac cgattttgcc aggttacgcg gctggtcaac
      421 gtcggtgcct ttgatcagcg cgacgtgata agccagtaac tgcagcggaa cggtgtagaa
      481 gatcggtgca atcacctctt ccacatgcgg catctcgatg atgtgcatgt tatcgctact
      541 tacaaaaccc gcatcctgct cggcgaagac atacaattgg ccgccacgcg cgcgaacttc
      601 ttcaatgttg gatttcagtt tttccagcaa ttcgttgttc ggcgcaacga cgataaccgg
      661 catatcggcg tcaatcagcg ccagcggacc gtgtttcagt tcgcctgcag cgtaggcttc
      721 agcgtgaata taagagatct ctttcagctt caatgcgcct tccagcgcga ttgggtactg
      781 atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc
      841 aatgcgtttg tcctgagaca gcatctgctc aatacggctc ggcagcgcct gcagaccatg
      901 cacaatgtca tgttcaatgg aggcatccag acctttcagg cgagacagct tcgccaccag
      961 catcaacagc acagttaact gagtggtgaa tgctttagtg gatgccacgc cgatttccgt
     1021 acccgcgttg gtcatcagcg ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt
     1081 acagattgcc agcgaaccaa ggtaacccag ctctttcgac agacgcaggc ccgccagggt
     1141 atccgcggtt tcgccagact gtgacaaggt gatcatcagg ctgttacgac gcacggcaga
     1201 tttacgatag cggaattcag aggcgatttc gacgtcgcac ggaatacctg ccagcgattc
     1261 aaaccagtaa cgggaaacca taccggagtt ataagaagta ccacaggcga ggatctgaat
     1321 atgctcaacc ttcgacagca gttcgtcggc gttcggtccc agctcgctta aatcaacctg
     1381 accgtggctg atgcgcccgg taagggtgtt tttgatcgcg ttcggctgtt cgtagatctc
     1441 tttctgcatg tagtgacggt aaataccttt atcgcccgcg tcatattgca gattggattc
     1501 gatatcctga cgttttactt ccgcgccagt tttatcgaag atgtttaccg aacggcgagt
     1561 gatttccgca atatcgccct cttcaaggaa gataaagcga cgggtcaccg gcagcagcgc
     1621 cagctggtca gaagcgataa agttttcacc catacccaga ccaatcacca gcggactacc
     1681 agaacgtgct gccagcaggg tatccgggtg acgggagtcc atgatcactg taccgtacgc
     1741 accacgcagt tgcgggatag cacgcagaac agcctcacgc agagtaccgc cctgtttcag
     1801 ctcccagttc accagatggg caatcacttc ggtgtcggtt tcagaaacga aggtatagcc
     1861 acgcgctttt aactcttcgc gcagcggttc atggttttcg atgatgccgt tatgcaccac
     1921 cacaatgtgt tcagaaacat gcggatgcgc attcacttct gaaggttcac cgtgggtcgc
     1981 ccagcgagtg tgagcaatac cggtgccgcc atgcagagga tgttcttccg ctgcctgagc
     2041 cagcatctgg actttaccga ggcgacgcag gcgggtcata tgaccttccg catcaacaac
     2101 ggccagaccg gcagagtcat atccgcggta ttccagacga cgtaaacctt caagaaggat
     2161 ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca cacatagttt ttgattccga
     2221 tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg ggcgccccga accttgtaga
     2281 gagtggggtt atttttatag ttactgcttg tgggagggag attatgttat ctcctcatcc
     2341 catgtgaccg gattacccgg ccagaatcac tttttcttta ccggacgacg ccagccttct
     2401 ttctgagtct gcggcacacg gctgatagct aatgcatttt cgccgacatt acgcgtcaca
     2461 gttgtacccg cagcaatggt cgcgcctttg cctactgtta ccggggccac cagctgagtg
     2521 tcggaaccga caaacacatc gtcgccgata atggtcttaa atttattcgc accatcgtag
     2581 ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc caatttccgc atcgccaagg
     2641 taagtcagat gaccagcttt cgagccttta cccagacgtg cttttttcat ctcaacaaag
     2701 ttaccgacgt gtgcaccttc caacaactca gcaccaggac gcaaacgggc aaacgggcca
     2761 atggtacagg ccgccgccag attcgcgtct tccacgacgg tatatgggct aatttcgcaa
     2821 tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc cgattttcac gcgatggccg
     2881 agagtcacgt tgccctcgat gataacgtta gtatcaattt caacatcgcg cccgtgagta
     2941 agcgtaccgc gcagatcaaa acgcgccgga tcgcgcagca taacgcctgc taacagcagt
     3001 ttttcagcct gttcggactg gtaaacgcgc tccagacggg agagctgcag gcggttattc
     3061 acgccttcta cttcgcttaa acgttgcgga tgaacggcga cgatttcacg cccttcctga
     3121 tacgccagcg caataatgtc ggtgatgtag tattcgccct gcgcattatt gttggtcagc
     3181 ttcgccagcc agcgtttcat atctgcgccg ttagcgatca gaatgccggt gttgatctcc
     3241 tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa caatgccggt aactttgccg
     3301 ttttcacggg tgatacgtcc ataaccggtc ggatcatcca gtttcaccgt cagcagacca
     3361 atgccaccct gcggtttagc atcacgcaga cgctggagtg tttcgacaga gatcagcggc
     3421 acgtcgccgt agagcattaa aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc
     3481 atggcatgac ccgtacccag ctgctctgcc tgaagcaccc agttcaggtt gtcgtctttc
     3541 agcgcctgtt ttagcagatc gccaccgtga ccgtacacca ggtgaacgtg cgctgcgcct
     3601 aattcattcg cagcatcaat gacatgctga accatcgctt tcccggcaag ggtatgcagc
     3661 actttcggaa gatcggaata catgcgcgtg cctttacctg cggcaaggat cactacgctc
     3721 atagcattat tcaacatacg cgtcctgact gtaatttgag aacgaattta aaccgcttca
     3781 ccttgaaaaa actacatttt tttcatcgtg aaatggacag aggataaatt gttcaataac
     3841 ggattatccc cgctgacgaa agcgccattt tcgaccatcg tcacgccttt tgtctgcaga
     3901 aaattaaggc agtaccttca tttaagcgtc aggtggatgt ttttgctctt atttcgatca
     3961 atgaataaac gggaaaacag ggggttttat atcagcagga tctatgtgaa cgctattcag
     4021 gacgggtcac acgcgcaaaa aaaagccagc ctgtttccag actggctttt gtgcttttca
     4081 agccggtgtt acatcgcttt tttggtcaac tcgataacgc gcagctgcgc gatcgctttg
     4141 gccagttccg cagacgcctg agcgtaatct acgtcgccgt gagagctgct aatgtgctct
     4201 tcagccttac gtttcgcttc catggctcgc gcttcgtcga gatcctggcc gcgaattgcg
     4261 gtgtcggcca gaacggtcac gttgccaggc tgcacttcaa gaatgccgcc agacagatag
     4321 ataaactctt cgtgaccgtg ctgtttcacg atgcgaatca taccaggctt aatggcggtg
     4381 agcagcggtg cgtggccagg gtagatcccc agttcacctt cgctacccgt tacctggatt
     4441 ttctcgacca gaccagagaa catttgttgc tctgcgctga cgacgtccag gtggtaagtc
     4501 attgccatat caccctccga ttaaggcgtt aaagtttttt ggctttttcc acagcttctt
     4561 cgatggaacc gaccatgtag aacgcctgct ccggcaggtg atcgtattcg ccttccatga
     4621 tgcctttaaa gccacggatg gtgtctttca gggagacgta tttacccgga gaaccggtga
     4681 atacttctgc cacgaagaac ggctgggaca ggaagcgctg gatcttacga gcacgcgcta
     4741 ccaccagttt gtcttcttca gacagttcat ccatacccag gatggcgatg atgtctttca
     4801 gttcctgata acgttgcagg atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac
     4861 caaccaccag cgggtccagc tgacggctgg tggagtccag cgggtcaacg gccgggtaga
     4921 tacccagaga cgcgatctga cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg
     4981 ttgccggaga cgggtcagtc aagtcatccg caggtacgta tactgcctgt acggaggtga
     5041 tagaaccggt tttggtggag gtgatacgtt cctgcagaac gcccatctct tccgccaggg
     5101 tcggctgata acctaccgct gaaggcatac ggcccagcag tgcggatact tccgtaccgg
     5161 ccagggtgta acgatagatg ttgtcaacga acagcagaac gtcacgacct tcgtcacgga
     5221 atttctcagc catggtcaga ccggtcagag ctacgcgcag acggtttccc ggcggctcgt
     5281 tcatctggcc atacaccagg gatactttat cgataacgtt ggagtcggtc atttcgtggt
     5341 agaagtcgtt cccctcacga gtacgttcac ctacgcccgc aaacacagag taaccggagt
     5401 gctcgatcgc gatgttacga atgagctcca tcatgtttac ggttttacct acacccgcac
     5461 caccgaacag accaacttta ccgcccttag cgaacggaca catcaggtcg ataactttga
     5521 taccggtttc cagcagttcc tgagagtttg acagctcttc gtaggaaggt gctgcgcggt
     5581 gaatcgccca acgctcttct tcaccgatct cgcctttcat gtcgaccggt tcacccagta
     5641 cgttcatgat acggcccaga gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt
     5701 cttttacatc cagaccgcga cgcagaccgt cggaggaacc cattgcgatg gtacgcacga
     5761 taccgccgcc gagctgctgc tgaacttcca gcaccagacg ctcattacca ttttgcacct
     5821 caagagcatc gtacacgcgc ggtacggcat cctgagggaa ttcgacgtca actacggcgc
     5881 cgattacctg gacaatcttt ccagtagcca tcttaaatcc tctacgaaat aacctgttta
     5941 aaccgcggcg gcccccgaga cgatctcggt gagttcctga gtaatgctgg cctgacgagc
     6001 tttgttgtat accaactgca gctctttaat caggctgccg ccattgtcgg tcgcggcttt
     6061 catcgccacc atacgggcgg cctgctcgct ggccaggttt tcaaccacgc cctgataaac
     6121 ctgagattcg acataacgac gcagcagggt atccagcaac gccttcggat cgggttcgta
     6181 caggtaatcc caggatttat gtttcagatc atcatcatct gatgccggta acggcagcag
     6241 ctggctgatg gtcggaacct gagacatggt gttaataaat ttgttgctga caatgtaaag
     6301 cttgtccaga cggccttcgt cgtaggcctg caacatcact tttaccggac cgatcagttc
     6361 ggacagggaa gggttatccc ccatgccggt gacctgggca acaacattgc cgcccacgga
     6421 gttgaagaac gacacgcctt tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc
     6481 ggtccaggtc ttcatttccg ccagcagttt tttgaacagg ttaatgttca aaccaccgca
     6541 caaaccacgg tcggtcgaca ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag
     6601 gtaagggtgc ttatattcca gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt
     6661 ttctgcataa ggacggctgg ccgccatgcg atcctgcgat ttacgcattt tggaagcggc
     6721 gaccatctcc atcgctttag tgatcttttg cgtgttctgg acgcttgcga tcttactacg
     6781 tatctctttt gcgccggcca tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa
     6841 gccgccagac gttaccagga ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg
     6901 ccttcgattt cgtcgttgta gccaccggtc tggttgatct cttgcatcaa cggagcgtga
     6961 tcacggtcga cgtaagccag cagagcggct tcgaagctgc cgattttcga cagttcaaca
     7021 tccgccaggt aaccacgttc tgctgcgaac agaaccagag actgctgcgc aacggacatc
     7081 ggcgcatact gtttctgttt cagcagttcg gtcacttttt gaccgtggtc aagctgctta
     7141 cgtgttgcat cgtcaaggtc ggatgcaaac tgagagaacg ctgccagttc acgatactgt
     7201 gccagagcgg tacggatacc accggacagt tttttcatga tcttggtctg tgctgcacca
     7261 ccaacacggg atacggaaat acccgggtta accgcaggac gaataccggc gttgaacagg
     7321 ttggtttcca ggaagatctg accatcggta atggagatta cgttggtcgg aacgaacgca
     7381 gaaacgtcac ccgcttgagt ttcgataatc ggcagcgcgg tcagtgaacc ggttttccct
     7441 ttcacttcac ctttggtgaa agcttcaacg tattcggcgt taacacgcgc agcacgctcc
     7501 agcagacgag agtggaggta gaatacgtcg cccgggaatg cttcacgtcc tggcggacga
     7561 cggagcagca gcgagatctg acggtaagca acagcctgtt tagacagatc atcgtaaatg
     7621 atcagcgcat cttcaccgcg gtcacggaag tattcgccca ttgcgcaacc ggcatacggt
     7681 gccaggtatt gcagtgcagc ggattcagac gcggttgcta ccacaacgat ggtgttagcc
     7741 agtgcgccgt gctcttccag tttacgtacc acgttagaaa tggtggacgc tttctggccg
     7801 atagcgacat agatacattt gataccggaa tcgcgctggt tgatgatggc atcgatagcc
     7861 agtgcggttt taccggtctg acggtcaccg atgatcaatt cacgctgacc acgaccgatt
     7921 gggatcatgg agtcaacggc tttataaccg gtctgtaccg gctgatctac ggactgacgt
     7981 tcgataacgc ccggagcgat tgcttctaca gcagagaagc cgtcgtgatc cagcggacct
     8041 ttaccgtcga ttggtgcacc cagagtgtta accacacggc ccagcaggcc acggccaacc
     8101 ggaacttcca ggatacggcc agtacactta actttcatgc cttcggcaag gtcagcatac
     8161 ggacccataa caaccgcacc aacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg
     8221 ttacccggca gggagatcat ttcaccctgc atacaatcgg ccaggccgtg aatgcggata
     8281 acaccgtcac ttacagaaac aatagtacct tcgttgtgag cttcactcac aacattgaac
     8341 tgagcaatgc gctgcttgat cagttcgctg atttcggtgg aattcagttg catgctccag
     8401 tccccttaag actgcaagac gtctgcaagg cgctcaagac gaccgcgtac gctgccatca
     8461 atgaccatat cacccgctcg gatgataacg cctgccatta cagacttatc gattttgcaa
     8521 ttcagcttaa ctttgcgtga cagacgtttt tccatcgcag cagaaatttt cgcgagctgt
     8581 tgttcactca gtgcggcagc ggaaatgacg tctacctcag cggtagcctc actcacggca
     8641 cgaaggtgaa taaactgctc caaaacatcc gggagcgcgt taagacgacc attttcagcc
     8701 attacccgaa taaggttctg accgttttcg tccagttgct caccacaaac tgcgataaac
     8761 gactcggcaa gcgtttctgg cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt
     8821 ttggttacct cggcggcaaa cgccagcatg tcctgccagc gttctacact ttggtgttcg
     8881 acggcaaagt caaaagctgc tttggcgtag gggcgagcta ccgtaataaa ttcagacatc
     8941 agcccctccc tccttacagt tcagcgacaa gtttatccac gacgtcgctg ttagcagctt
     9001 catccacgga acgttcgatg atcttctcgg cgccagcaac agccaggata gcaacttgct
     9061 tacgcagctc ttcacgggca cgtttacgct cggcttcaat ttccgcctgc gcctgggcca
     9121 cgattttagt acgttcctgt tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc
     9181 gtttgttcgc ctgctcgatg attacctggg cttccgcttt cgcttttttc agctggtcgg
     9241 tcgcgctggc ctttgcaagg tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt
     9301 cagcaatttc tttttgacgt ttttcgatgg ctgccattaa tggcggccat acgtacttca
     9361 tgcagaacag aacgaacagg acaaacgcga tggcctggcc gaggattgtt gcgttaagat
     9421 tcacagcaca atgcctctat ttagttaacg ttctgatatt gctctttaaa taaaagcaac
     9481 gcttactacg cgacagcgaa catcacgtac agacccagac ctacagcgat catcgggata
     9541 gcatccacca gacccataac gataaagaac tgagtacgca gcagaggaat cagatcaggt
     9601 tgacgcgctg cgccttccag gaatttaccc ccgaggatgc cgataccgat cgcagcaccg
     9661 attgccgcca gacccatcat cacagcggca gccatgtaca gcagatccat attcaggttt
     9721 tccatgacag tctccagttt gtttcagtta aaacgtagta gtgttggtaa attaatgttc
     9781 ttcagacgcc atcgacagat agacgatcgt cagaaccatg aagatgaagg cttgcagcgt
     9841 aatgatcagg atgtggaaaa tggcccacgg cacattcagg atccactgtg accaccacgg
     9901 caacagacca gcaatcagaa tgaaaatcag ctcaccggca tacatgttac cgaacagtcg
     9961 caaaccgagt gaaactggtt tggacagcag gcttacccct tcaaggatta agttgacagg
    10021 aatgaacgcc cagtgattga acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc
    10081 tttcattttg atgctgtaga acagaatcag gataaatacg cccagtgcca tagacagcgt
    10141 tacgttcacg tccgcagacg gaaccacacg cagtgcaggc agacccagta catgttcagc
    10201 aatgtacggc agcaggtcga taggcagtaa atccatcagg ttcatcagga atacccagac
    10261 gaagatcgtc agggccagcg gagcaatcag cttgcttttg ccatggtaca tgtctttcac
    10321 gctaccatta acaaagccga tcaccagctc aatcgcggtc tgaaacttac ctggcacacc
    10381 gctggtcgcc tttttggcta cgctacggaa taaaaccagg aacaacagac ccagcaccac
    10441 cgagaagaac atggagtcaa tattgattgt ccagaaggtg gctggggggt tttgtggatc
    10501 caccagcgag aatgtacgca ggtccagctg aaggttattc aggtggtgtc ctatgtaatc
    10561 ctgcggcgtc atattttctg aagccatgat gccttttacc ctttgttgtt aattacagcc
    10621 ggtgccagta tctgaaccac cagcaccaaa acccacgtaa cgatcagcgg caagaatacc
    10681 gcctttaaaa ccgccaacgc caccaccagt aacaccaaca tcgccagaac tttgaaagct
    10741 tcaccaaatg cgaatgtcca ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc
    10801 caggcaaata tcataaacaa aacgttaggc agaaagactg ccaggccccc gcttattgca
    10861 gagacgcccc agaaggggtc tttgaggctg aacagcaatc cacttgctat caccaccagt
    10921 aactgaacga gcagaagctt ccgagcaacg tttcgactca cgagcgacac agacatcacg
    10981 tttttcactc ctgctccctt cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct
    11041 tagagtcaag catcaaaaag cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat
    11101 aagtagccaa aaggtgaata aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt
    11161 tctactaacc ctgttcgatc atgaaaaact gtaaataacg cgtaaacact ggtgataaag
    11221 cgtgcttcag atcacatatt gcgcatgttc gcgcacagca tatttattta cttggcaaat
    11281 gatgcctttg caagtttatg atatttcagt ctaaaaacag atactgtttt aataaatgac
    11341 atttacacaa caaaaaccac ccattgacat ttttaataat gttttaacag ccaatgatgg
    11401 ttcttagcgc cgatttttag cagactgata ttttcactaa tgacttattt tctgcttacc
    11461 aaaaaaagcc acgttatctt gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag
    11521 ttatatcagt agaaaacctg gttgttgtta acagtctaac cggtcaattt tttatgattt
    11581 ttttgataaa aattaaattt tatttgcttt aatcaccacc agatgacgtt cgccatccag
    11641 ggctggaacc tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcgat
    11701 ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac caggaagatg
    11761 gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa
    11821 tggcggctct gaaggaaact cttctaccct gctctgtact ggctcaatat tctccagttt
    11881 aagctcatgt tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacagagt
    11941 gaaatgggct tcaggacgca cgatagagag tggaatgcca ggcagtccag gaccggtgcc
    12001 gacatcgata aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg
    12061 gcgtaccagc atctcattag gatcgcggac cgaagtcagg ttgtacgctt tgttccattt
    12121 atgcagcata ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc
    12181 tgcgtctttc agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt
    12241 tgcctggtaa gcgggggctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat
    12301 accctgtttt ttcagccaca ccagcagaat ggagatggcc gcaggcgtga cgccagaaat
    12361 acgtgaagcc tggccgatag aggccggttt gtgatcgtta agtttggcga tcacttcgtt
    12421 agaaagaccg gatacctggc ggtaatccag tgtcgcgggt agcaaggtgt tctcgttacg
    12481 cagctgcttt tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat
    12541 ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt
    12601 taatttttca taagtcattt ccggacgacg cagcagatct tcaccactgg cttcacggga
    12661 aagcggcgca gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca
    12721 ggtcgatttc agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc
    12781 ccaacgttcg tcatccacca ggcccagttc acgaccgatt tcagtcaaac gcagatccgc
    12841 attatcttcg cgcagcatca gacgatattc tgcgcgcgag gtaaacatac gatacggttc
    12901 tttggttcct aaagtgcaca ggtcatcaac cagtacgccg agataggcct gagaacgcgc
    12961 cggagcccaa ccttctttgt catcagacag acgggcagcg ttaagaccgg ccagcaaacc
    13021 ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc
    13081 ctggataaac ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga agtcatactc
    13141 aatggcataa cccggacgca cgatcttcgc gttttccatc ccctgcatag agcggacgat
    13201 ttgcatctgc acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcgttaga
    13261 agtcagccct tccggttcaa ggaagatctg atgctgattt ctgtcggcga agcgcatgac
    13321 tttgtcttcg atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat
    13381 tgggctacga tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgggtgat
    13441 ataacacggc acctgctgtg gatgctggga cgcattgccc ataaaagaga ataccggcat
    13501 tgggttatcg ccatgctgtt gcgcaagcac gctaaagtcg atggttcgag catcaatacg
    13561 cggtggtgtc ccggttttca gacgaccaac gcgcagcgga agttcacgca aacggcgaga
    13621 aagcggaatg gacggcggat caccagcacg gccaccgctg tagttatcca gaccgatatg
    13681 aattttaccg tcgaggaacg tcccaacggt gagcacgacg gctttggcac ggaacttcag
    13741 tcccatttgg gtaacagcac cgaccacacg atcgttttcg acaataagat cttcaaccgc
    13801 ctgctggaag atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta
    13861 gagcacacga tccgcctgag ctcgggtagc gcgaactgcc ggtcccttgc ttgcgtttag
    13921 tatcctaaac tggatacccg cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc
    13981 cacttctttt accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg
    14041 ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcagc
    14101 catcgcggcc tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg
    14161 ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt
    14221 ggattctact caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaagaag
    14281 atctatttat ttagagatct gttctattgt tatctcttat taggatcgca ctgccctgtg
    14341 gataacaagg atccggcttt taagatcaac aacctggaaa ggatcattaa ctgtgaatga
    14401 tcggtgatcc tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa
    14461 ctgaacaacg gttgttcttt ggataactac cggttgatcc aagcttcctg acagagttat
    14521 ccacagtaga tcgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca
    14581 ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc
    14641 cgtctgtttt gcaccggaat ttttgagttc agcctcgagt ttatcgatag ccccacaaaa
    14701 tgtgtcatat tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg
    14761 cttctgttcc tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc
    14821 gtgggtggag ctgataacca gccagatccc cgaggcagat aaatcttcca acagcggacc
    14881 gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt tcagccaggt gttctgctac
    14941 atattcggca ccgccgaggg tgctgccgct gataagagtt atatctgcca taaaccgcca
    15001 cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag
    15061 tatgggatta aaaaagccga tcagggcttg atggtacgca tgatcgggtt ctgcaggacg
    15121 atcagtgtct cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc
    15181 tggagagcat cgatcgaacg gcacatcact tttataaaga tgctgtagtg gccggtggtg
    15241 tagtacgcct cggtcacttc atcgaggctt tccagctttg ccagcgcgga agggtagtct
    15301 ttggcgctct ttaatataat gccgataaag cagcctacgt cataaccgag ctgcttcggg
    15361 ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tactcgaacg
    15421 tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca
    15481 ttgcccatta atgcttccag gatgccacgg tccagattgt cgatcagata attttccata
    15541 ggattttctt atgcggattg atgattcatt ctattttagc cttctttttt aatgaatcaa
    15601 aagtgagtta ggctttttat tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat
    15661 cataagcaac aggacgcagg agtataaaaa atgaaaaccg cttacattgc caaacaacgc
    15721 caaattagct tcgtgaaatc tcacttttct cgtcaactgg aagaacgtct ggggctgatc
    15781 gaagtccagg cgccgattct tagccgtgtg ggggatggca cgcaggataa cttgtcgggc
    15841 tgtgaaaaag cggtacaggt aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt
    15901 cattcactgg cgaagtggaa acgtcagacc ttagggcaac acgacttcag cgcgggcgaa
    15961 gggctgtaca cgcacatgaa agcccttcgc cccgatgaag accgtctttc tccgttgcac
    16021 tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc
    16081 tcgactctga aaagcacggt agaggcgatt tgggcgggaa ttaaagcaac cgaagctgcg
    16141 gttagcgaag agtttggcct ggcaccgttc ctgccggatc agatccactt cgtacacagc
    16201 caggagttac tgtctcgtta tccggatctt gatgccaaag ggcgtgagcg ggcgatagcg
    16261 aaagatcttg gtgcggtatt ccttgtcggg attggcggca agctgagcga tggtcatcgc
    16321 cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc cgtcagagct gggccatgcg
    16381 ggtctgaacg gcgatattct ggtgtggaac cctgtactgg aagatgcgtt tgagctttct
    16441 tccatgggga tccgcgtgga tgccgacacg ttgaagcatc agttggcgct gaccggtgat
    16501 gaagatcgtc tgcagctgga atggcatcag gcgttgctgc gcggtgaaat gccgcagacc
    16561 atcggcggcg gtatcggcca gtctcgtttg accatgctgc tgctgcaact gccgcatatc
    16621 ggccaggtcc agtgtggagt atggccagct gctgttcgcg agagcgtccc ttctctgctg
    16681 taataattta tcgccgccag cgtctgagca ggcggcttcg catcccggta tcaaagcgcc
    16741 agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg tgccgacatc gccacggcat
    16801 gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt actcgtcacg tcgtcaggca
    16861 accgctgggc gataaaatca gaaatcacca ccgcatcggc atcaaaccat tccctgcttt
    16921 gcaagcgttc cataatggcg cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct
    16981 ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc tgaaagctca taacggacga
    17041 tctcggtgga aaatagcata atataacagc gccggttttc agcgagagca atgcgcatca
    17101 aagccaggca gaacgctttc gcacactgtt cattaaagcc gcccattgag ccggaagtat
    17161 ccacgcagac aataaacggt ccgcgcggct gttcgtcgta atctttatgc accaccgggc
    17221 gttcgatcac tttttcacgc cacgactcac cgtgcaggcg ataggtgagc aactgttttt
    17281 ccaccagccg acggtaaaac tcatactcca gttccgttat ccctagtgtc gccagttctg
    17341 gcggcagaag acgtaaaata tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg
    17401 tcgccggttc gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg cgcggtattg
    17461 atttggcttc ccgggaacgc cccagttgtt ctgccaggcg tttcagttcc ggctgttcgt
    17521 taagaaattc accgtatttc acaatcaact gatagtcgcc acgtttaagc tgaccggcgc
    17581 tcatatccca cagacgacca gctgcggtat tgttatctgc gagaatcggt tcaagttgtc
    17641 cgctcagcgt catgcgttcc tgaacttcac tcaacagttg ttcgcgttct tcttctaata
    17701 gctgttgatt taacgttgtt gcttgcacga tcagacttaa acgccagcgc tggagaaaaa
    17761 gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcgac caactgtcgg gcttgttctg
    17821 cccatgggga attcagacga tgcagtaagt ccaggatctg tggtagctgc acaataaact
    17881 gcggcgtgga gaggagctgg ctttgctgat agcacatcac ctcttcggtg agttccggcg
    17941 ggactcgggc atctttcagc cgactgcgca gcgcctcacg ccagcgggga acatcatcag
    18001 tgattgccgc cttcagtcgt gggaattttt caaagaagac tgccagctgc ggtgaggcca
    18061 gcagcgcgat gatcatctct tcgatcaatc cctcttcgct gacggccagc atcacattaa
    18121 gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct
    18181 ggcttcaatg cgacctaacc agtcgccagg gataaacaga catctttgct gttcgctgaa
    18241 taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc aattgttgtt ttatttcacc
    18301 tggcagacct tcagccgatg aaccgggaag tgccagcgta ctgccttgta aacttacatc
    18361 gcgtacaaca agatgttggg cgctatcaac ttccagattc agtttctggg caaagccgat
    18421 accgttcagt ttgccgcgaa tttcaccacc tttgctcagc cactgttcca gcgcgctacg
    18481 ctcaaaggag atatgaacca cttccatatt atgcaatttt aacggttttt gcagcagcag
    18541 agtcagagtg gaagcagtaa cgttaacagg aagttgatac tgctgacgac ggctgaaaat
    18601 gccgcccaga cgaattaccg ttaaggctgt tttatcgctt tgttgctgct ggagttgcag
    18661 gtgacgttgc acaatcgcgc ccaggcgggt caacatccct tgctgttgcc aggcatgacc
    18721 ggtcatcaat acatcaattt gttgttgtat caaattcagg ctttgcgcgt cataccacag
    18781 gcaatctttc agcaaaatga gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc
    18841 gctggcctgc aataatcgaa tcgctttttt ccagcgacga tccgagacat aaggcgcatc
    18901 cggtaattta tccagttgct ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag
    18961 cgtaatttca ccaatctctt tttgccagcg ttcatattct tcatctgtga cctgcaagga
    19021 ggctggaacc ggattgtcat tttcatcctg ttgactggtc agcatggagc ggaaattcgc
    19081 tttatcctgc actttatcta accacagacg aatcagcatg cggtcatata acgcttccag
    19141 actgctgtct gcttccggca gctcgttgga ggccgccacc agcaggcgca tcgggatttt
    19201 ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg gcggtgagca gggtattaag
    19261 aattgccgga cccgctttcc agatctcatc cagaaagacg atttctgctt ccggcaggta
    19321 accgctggtt aaacgttcat agcgcccttc atcttttagc gcctgaatag aaaggggacc
    19381 aaaaacttct tccggcgtgg agaagcgggt catcagatat tcaaacgcgc gggcattctg
    19441 aaaggcgaat tttaagcgcc gggcgatcaa acttttggca atacctggcg ggccaaggag
    19501 gaacacactt tcaccactta atgccgctaa taaacacaag cggatggcgt ggctacgttc
    19561 ataaagcccc ttttccaacg aactgctcag gcgggaaatt ctttccgcta ataaatgagg
    19621 gtgagccata atgaagtggc gtcctttcgt caaaagttct gcgtaaattg cgagtataga
    19681 cgtttcttgc tggtggctaa aatagtctca aagggggggt atttttcttt gagccaggtt
    19741 aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt
    19801 gtgggccaag ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact
    19861 gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat
    19921 attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc
    19981 gttgaacgcg atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg
    20041 gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga aggggggatc
    20101 ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg
    20161 attatggggc taatcggcgg cagctttttt tatggtgaag tcgtcataac acccgctatt
    20221 tcggtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata
    20281 gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaagca tggcacggct
    20341 atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg
    20401 gggttacgta gcattattgc taaccaggaa gtgctgtatg cgctgaatcc gatgtgggcg
    20461 gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg
    20521 tcgattacgg gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt
    20581 cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga
    20641 gcgctgttgt taaagaatcc ggaagcgatt aagaacccgt tcttcctttt ggcaccggac
    20701 tgggcgctga tcccgctgct gatcatcgcc gcactggcga cggtaatcgc ctcgcaggcg
    20761 gttatctctg gcgtcttttc attgacgcgt caggcggtac gtctgggata tttgtcgccg
    20821 atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac
    20881 tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg
    20941 gcggcggcgt acgggattgc ggtgaccgga accatggtgc tgacgtctat tctctcgact
    21001 accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat cttgattgct
    21061 ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc
    21121 tggttgccat tgagcctcgg tacggtgatg tttatcgtga tgaccacctg gaagagtgag
    21181 cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct
    21241 tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtatatat gtcgcgtgca
    21301 atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag
    21361 cgggtgattc tgttaactct gcgcaccgaa gacgctccat atgtccataa cgtccgtcgg
    21421 gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga
    21481 gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg
    21541 atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg
    21601 tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca
    21661 gatcaatttg aaatcccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa
    21721 cgccagacgc ctcctttctt cataaggggg cgtttttgtt ttcatggtta atcaccatgt
    21781 aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca
    21841 ggattaaact gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac
    21901 cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt
    21961 ggtgtgtgat gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac
    22021 ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga
    22081 ggctgccatt atcgcggaag agatcaaaca ccataatccg caactccacg aaacgttgct
    22141 cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac
    22201 gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg
    22261 ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag
    22321 cattacttca gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg
    22381 cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta
    22441 aatccaccat gatgaaagtg cttactggca tctatactcg cgatgccggt acgcttttat
    22501 ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga
    22561 ttatccatca ggaactgaac ctgatcccgc agttaaccat tgccgaaaac attttcctcg
    22621 gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg
    22681 ataaattgct ggctaaactt aacctgcgct ttaaaagcga caagctggtg ggcgatcttt
    22741 ccatcggtga ccagcaaatg gttgaaatag ccaaagtgct gagctttgag tcgaaagtca
    22801 tcattatgga tgaaccgacc gatgcgctga ccgataccga aaccgaatcc ctgttccgcg
    22861 tcatccgcga gctgaaatcg caaggccgcg gtattgtcta tatctcccac cgcatgaaag
    22921 aaatcttcga gagttgcgat gacgttaccg tttttcgtga cggacaattt attgctgagc
    22981 gcgaagtggc atcactgacc gaagattcgc tgattgagat gatggtgggt cgcaagctgg
    23041 aagatcaata tccgcacctg gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc
    23101 tctgcggacc tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg
    23161 tctctggttt gatgggcgca ggtcgtaccg aactgatgaa agtgctctac ggcgcactac
    23221 cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg
    23281 atggcctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt
    23341 tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg
    23401 gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgattttatt cgcctgttca
    23461 atgtgaaaac gccgtcgatg gagcaggcaa ttggtctgct ttccggtggc aatcagcaaa
    23521 aagtggcgat tgcccgcggt ctgatgacac gccccaaagt gttgatcctt gatgaaccta
    23581 cccgtggcgt agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag
    23641 ccgatggctt gagcatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg
    23701 atcgcatcat cgtcatgcat gaagggcatc tcagcgggga atttactcgt gagcaggcca
    23761 cccaggaagt gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa
    23821 aaaatgacaa cccagactgt ctctggtcgc cgttatttca cgaaagcgtg gctgatggag
    23881 cagaaatcgc ttatcgctct gctggtgctg atcgcgattg tctcgacgtt aagcccgaac
    23941 tttttcacca tcaataactt attcaatatt ctccagcaaa cctcagtgaa cgccattatg
    24001 gcggtcggga tgacgctggt gatcctgacg tcgggcatcg acttgtcggt aggttctctg
    24061 ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg
    24121 gctgtcgctg ccgctctcgc gttaggtgcc gcaattggtg cggtaaccgg agtgattgta
    24181 gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta tgatgctttt actgcgcggc
    24241 gtgaccatgg tttataccaa cggtagccca gtgaataccg gctttactga gaacgccgat
    24301 ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg
    24361 gggattgtct tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc
    24421 tacgcgctgg gcggcaacga agcggcaacg cgtctttctg gtatcaacgt caataaaatc
    24481 aaaatcatcg tctattctct ttgtggtctg ctggcatcac tggccgggat cattgaagtg
    24541 gcgcgtctct cctccgcaca acccacggcg gggactggct atgagctgga tgctattgct
    24601 gcggtggttc tgggcggtac gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg
    24661 atcggcgcat taattcttgg cttccttaat aatggattga atttgttagg tgtttcctcc
    24721 tattaccaga tgatcgtcaa agcggtggtg attttgctgg cggtgctggt agacaacaaa
    24781 aagcagtaat aacgactaca ggacatcttg aatatgaaca tgaaaaaact ggctaccctg
    24841 gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc
    24901 gcgctggtgg tctccacgct taacaacccg ttctttgtat cgctgaaaga tggcgcgcag
    24961 aaagaggcgg ataaacttgg ctataacctg gtggtgctgg actcccagaa caacccggcg
    25021 aaagagctgg cgaacgtgca ggacttaacc gttcgcggca ccaaaattct gctgattaac
    25081 ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg ctaaccaggc gaacatcccg
    25141 gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg tgagccacat tgcttctgat
    25201 aacgtactgg gtggcaaaat cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc
    25261 aaagttatcg agctgcaagg cattgctggt acatccgcag cccgtgaacg tggcgaaggc
    25321 ttccagcagg ccgttgctgc tcacaagttt aatgttcttg ccagccagcc agcagatttt
    25381 gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag
    25441 gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc
    25501 ggtaaatcgg atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg
    25561 aatgatggca aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc
    25621 gttgaaaccg cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat
    25681 ctgaaactgg ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa
    25741 tacgtcatcg acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca
    25801 cggtggcgca ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg
    25861 gcagcattaa tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg
    25921 taaccggtaa ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg
    25981 ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac tggcgatgac agcattggcg
    26041 agagcgttcg ccagcagctc gccactgata acatcgatat tactccggtc agcgtgatca
    26101 aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg
    26161 gtattcatgc cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc
    26221 gtattgccaa cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg
    26281 cagcggcgaa aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc
    26341 gcgaacttcc tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag
    26401 cagaaaagct caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg
    26461 tactgcatga aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg
    26521 ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacca
    26581 ttgctgccgg agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat
    26641 tgccagaggc gattcggttt gcccatgctg ccgctgcgat tgccgtaaca cgtaaaggcg
    26701 cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac
    26761 gcttggctac aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc
    26821 acgttatcaa taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga
    26881 ttaaagaact caattacgcg ccatcagctc tggcgcgtag cctcaaactc aatcaaacac
    26941 ataccattgg catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg
    27001 gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt cctttgcaat accgaaggcg
    27061 atgaacagcg gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc
    27121 tgttactgtg caccgaaacg catcaacctt cgcgtgaaat catgcaacgt tatccgacag
    27181 tgcctactgt gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata
    27241 actcgttgct gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta
    27301 tcgcctgtat taccggcccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc
    27361 gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt
    27421 ttgaatttaa cggcgggttt gacgctatgc gccaactgct atcacatccg ctgcgtcctc
    27481 aggccgtctt taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg
    27541 cagagttaca ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa
    27601 gctttatgac gccaccatta accacgatcc atcaaccaaa agatgaactg ggggagctgg
    27661 cgattgatgt actcatccat cggataaccc agccgaccct tcagcaacaa cgattacaac
    27721 ttactccgat tctgatggaa cgtggttcgg cttagattta cgctgtcttt tgatcaaatt
    27781 attaccatcg gttgttttca gaagcatgaa cattgctgct gaagcaacag taataatgcc
    27841 cattgtgata aacgtatagt ggaattgttc gacagtcgtt gtgccttcca ttccttcata
    27901 aacgcgaagg acggccgcac ttacagctac gcctaaactg atagacagtt gctgcgtgac
    27961 cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt
    28021 attcatcgcg gtaaactgcg tcgacatagc catccctaat ataaacaacg gcaagatcag
    28081 catccatatt gccattgccg gtgattgcaa agagaactga gcgatcatta gcccaataat
    28141 caccgtgatc cccactaatg tatggcgata gcccagacga cgtaagactt gggtaaccat
    28201 cgattttgca ataatggaac ctaacgctgt cggtgccatc atacagccag caataaacgc
    28261 ctgataacca aatcctacct gtaacattaa tggcataagg aacggtacac agccggtccc
    28321 cagacgggtt gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc
    28381 taatgaaatt aatgggtttg gcgtgcgtcg tgcatggaga atatagagaa gcagtaaccc
    28441 gatgctggtg acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag
    28501 ctctattccg cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtgatatc
    28561 gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa gaaggcccgc
    28621 aatacctatg gggatattga ttaaaaatat ccagtgccag gttgcccagg tgaccagcac
    28681 gccgccaaga atggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa
    28741 tactggaaga agttcattac gaggataagc gcgcagtaag gccagccgag caacaggcat
    28801 catcattgcg ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgaatt
    28861 agaaagtgcg caggccagag aacccaatgt gaacagactc acggcaaggg taaaaatgcg
    28921 acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga ataagcatcg ccaccgtcag
    28981 cgtataactg atgatggctg attgcatcgc gagaggagaa cgattaaggc tatgagcgat
    29041 tgcgggtaag gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc
    29101 cgcgatccac ggcaaacccg ccatactgcg cttcttttta tcgctcattc aatgtcctgt
    29161 tatcgggtta tcacttatca ggtgagcgta gcagcgcctg acaagcttta aatgccgcgt
    29221 cgccatcgct ttggataatc gcatcgacta tcgcctgatg cagatccagc tttatcactg
    29281 tgtcgctggt aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg
    29341 aggtcaaaaa cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt
    29401 cgatccagcg ttcacggcga aagttctctt ttaatgccgc catttcggcc attaacgtat
    29461 tgagatgcgc tttctgttcc gcggtgccaa ccgttgctgc cagtaggcaa gcttgtggct
    29521 ccagacagat acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca
    29581 tccaccaggt aagcaattcc tgatcaagaa aattccagtt cgattgtggc atgacccggg
    29641 taccaattcg cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc
    29701 gtaccgctgt acgactcact ccaaattgct cgcccagctc aatctcaccg ggcaaaatgg
    29761 tgccgggttc atattcacct tttaagatcc gttgcgccag cttctcagcc agaacatacg
    29821 aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt
    29881 ctttttattc ctccttagta tgccaccagg aagtgtgatt acggttgcaa aaacggcaaa
    29941 ttgcttgttt tatggcacat taacggggct tttgctgaaa aaatgcgcgg tcagaaaatt
    30001 attttaaatt tcctcttgtc agaccggaat aactccctat aatgcgccac cactgacacg
    30061 gaacaacggc aagcacgccg ccgggtcagc ggggttctcc tgagaattcc ggcagagaaa
    30121 gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgccgctg
    30181 agaaaaagcg aagcgg
//
LOCUS       DAZGXS010000047        29987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGXS010000047 DAZGXS010000000
VERSION     DAZGXS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            134..1717
                     /gene="yegH"
                     /locus_tag="ACHKG5_002222"
     CDS             134..1717
                     /gene="yegH"
                     /locus_tag="ACHKG5_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0868326.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1792..2130
                     /locus_tag="ACHKG5_002223"
     CDS             1792..2130
                     /locus_tag="ACHKG5_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0868327.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2120..2410
                     /locus_tag="ACHKG5_002224"
     CDS             2120..2410
                     /locus_tag="ACHKG5_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0868328.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2463..4316)
                     /gene="asmA"
                     /locus_tag="ACHKG5_002225"
     CDS             complement(2463..4316)
                     /gene="asmA"
                     /locus_tag="ACHKG5_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0868329.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4338..4919)
                     /gene="dcd"
                     /locus_tag="ACHKG5_002226"
     CDS             complement(4338..4919)
                     /gene="dcd"
                     /locus_tag="ACHKG5_002226"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0868330.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5011..5652)
                     /gene="udk"
                     /locus_tag="ACHKG5_002227"
     CDS             complement(5011..5652)
                     /gene="udk"
                     /locus_tag="ACHKG5_002227"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0868331.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5970..9288
                     /gene="dgcE"
                     /locus_tag="ACHKG5_002228"
                     /pseudo
     CDS             5970..9288
                     /gene="dgcE"
                     /locus_tag="ACHKG5_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9389..10237)
                     /gene="alkA"
                     /locus_tag="ACHKG5_002229"
     CDS             complement(9389..10237)
                     /gene="alkA"
                     /locus_tag="ACHKG5_002229"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0868332.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10371..11723
                     /gene="yegD"
                     /locus_tag="ACHKG5_002230"
     CDS             10371..11723
                     /gene="yegD"
                     /locus_tag="ACHKG5_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0868333.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11736..13676)
                     /gene="yegI"
                     /locus_tag="ACHKG5_002231"
     CDS             complement(11736..13676)
                     /gene="yegI"
                     /locus_tag="ACHKG5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP0868334.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13673..14434)
                     /gene="pphC"
                     /locus_tag="ACHKG5_002232"
     CDS             complement(13673..14434)
                     /gene="pphC"
                     /locus_tag="ACHKG5_002232"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP0868335.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14431..15090)
                     /gene="yegL"
                     /locus_tag="ACHKG5_002233"
     CDS             complement(14431..15090)
                     /gene="yegL"
                     /locus_tag="ACHKG5_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0868336.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15311..15370)
                     /gene="ibsA"
                     /locus_tag="ACHKG5_002234"
     CDS             complement(15311..15370)
                     /gene="ibsA"
                     /locus_tag="ACHKG5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0868337.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15642..15698)
                     /locus_tag="ACHKG5_002235"
     CDS             complement(15642..15698)
                     /locus_tag="ACHKG5_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0868338.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15970..16026)
                     /locus_tag="ACHKG5_002236"
     CDS             complement(15970..16026)
                     /locus_tag="ACHKG5_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0868339.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16306..17553
                     /gene="mdtA"
                     /locus_tag="ACHKG5_002237"
     CDS             16306..17553
                     /gene="mdtA"
                     /locus_tag="ACHKG5_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0868340.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17553..20675
                     /gene="mdtB"
                     /locus_tag="ACHKG5_002238"
     CDS             17553..20675
                     /gene="mdtB"
                     /locus_tag="ACHKG5_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0868341.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20676..23753
                     /gene="mdtC"
                     /locus_tag="ACHKG5_002239"
     CDS             20676..23753
                     /gene="mdtC"
                     /locus_tag="ACHKG5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0868342.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23754..25169
                     /gene="mdtD"
                     /locus_tag="ACHKG5_002240"
     CDS             23754..25169
                     /gene="mdtD"
                     /locus_tag="ACHKG5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0868343.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25166..26569
                     /gene="baeS"
                     /locus_tag="ACHKG5_002241"
     CDS             25166..26569
                     /gene="baeS"
                     /locus_tag="ACHKG5_002241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0868344.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26566..27288
                     /gene="baeR"
                     /locus_tag="ACHKG5_002242"
     CDS             26566..27288
                     /gene="baeR"
                     /locus_tag="ACHKG5_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0868345.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27479..27811
                     /gene="yegP"
                     /locus_tag="ACHKG5_002243"
     CDS             27479..27811
                     /gene="yegP"
                     /locus_tag="ACHKG5_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0868346.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27959..29320
                     /gene="yegQ"
                     /locus_tag="ACHKG5_002244"
     CDS             27959..29320
                     /gene="yegQ"
                     /locus_tag="ACHKG5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0868347.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29650..29967)
                     /gene="yegR"
                     /locus_tag="ACHKG5_002245"
     CDS             complement(29650..29967)
                     /gene="yegR"
                     /locus_tag="ACHKG5_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0868348.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6936 a         8002 c         7904 g         7145 t
ORIGIN      
        1 ttcagggata atgtgaggtt attaccctca aataatatga gataaatagt gctgcaacat
       61 tgcatttttg ccccgattta tccatgatcg aattgtgaca tttgtcatac aacgaatagg
      121 ttttgtactt actatggaat ggattgccga tccgtctatc tgggccgggt taatcacgct
      181 gattgtgatc gaactggtcc tcggcattga taacctggtc tttattgcca tcctcgccga
      241 aaaactaccg ccgaagcagc gcgaccgcgc acgggttacc gggctgttgc tggcgatgtt
      301 aatgcgcctg ttactgctgg cgtcaatctc ctggctggtc accctgactc aaccgctgtt
      361 cagcttccgc tcgtttacct ttagcgcccg cgacttaatc atgctgtttg gtggtttctt
      421 cctgttgttc aaagccacga tggagctgaa cgaacggctg gaagggaaag acagcaataa
      481 tcccacacaa cgcaaaggtg cgaagttctg gggggtggtg acacaaattg tggtactgga
      541 cgccatcttc tcactcgact cagtgattac cgccgtcggg atggtcgacc atttactggt
      601 catgatggcc gccgtggtta tcgctatcag cctgatgttg atggccagca agccgttaac
      661 gcaatttgtt aacagtcacc cgaccatcgt tattctctgc ttaagcttcc tgttgatgat
      721 tggctttagc ctggtggcag aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc
      781 tgccattggt ttctcggtga tgatcgaggc gctcaatcag ttggctatct ttaaccggcg
      841 acgttttctt tccgctaacc agacactgcg ccagcggact accgaagcgg taatgcgcct
      901 gcttagcggg caaaaagagg atgcggaact ggacgccgaa accgcgtcca tgctggtgga
      961 tcatggtaac cagcagatct ttaatccgca ggaacggcgg atgattgagc gggtacttaa
     1021 tcttaaccag cgtaccgtca gcagcattat gacgtcgcgc cacgatattg agcatatcga
     1081 tctcaacgcg ccggaagacg agatccgcca gttgctggag cgaaatcagc atacgcggct
     1141 ggtggttact gatggcgatg acgcggaaga tttgctcggt gttgttcacg ttatcgacct
     1201 gttacaacaa tcactgcgcg gcgaaccgct caacctgcgg gtgttaattc gccagccgct
     1261 ggtgttcccg gaaaccttgc cgttgttacc tgccctggag cagttccgta atgcccgcac
     1321 acactttgct tttgtggtgg atgagtttgg ctcggtggaa gggattgtga cattaagtga
     1381 cgtcactgaa accattgccg gtaacttacc gaacgaagtg gaagagatcg acgcccgcca
     1441 tgatattcag aagaatgcgg acggttcctg gacggcgaat ggtcatatgc cgctggaaga
     1501 tctggtgcaa tatgtgccgc tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg
     1561 gctattgatg gaatatttgc agcgtattcc aaaacctggc gaagaagttc aggtggggga
     1621 ttatttgctt aaaacgttgc aggtggaaag ccatcgcgtg cagaaggtgc agattatacc
     1681 gctgcgtaag gatggcgaga tggagtacga ggtgtgataa aacatcaaat gcgcaggttc
     1741 aaactgcgca tttgatttat acaaaattta gtataatcta aggatggagt aatgagaaaa
     1801 aagctggctt ttcttgatac cagtcttgac gatttacgcg catttccaga atcgtctcga
     1861 caggaaattg gctatcaact cgacaggatc caacaagggt taaacccata cgactggaaa
     1921 cctttttcaa ctatcggccc tggtgtgcga gagattcgta ctcgcgatgc tgatgggatc
     1981 tatcgcgtca tgtatgtcgc aaaatttgaa gaagcggttt acgtattgca ttgttttcaa
     2041 aagaaaacac agactaccag tcaatcggat attgatttag ccaaacgacg ctataaagaa
     2101 cttgttcagg agcggaaaaa tgaaaattga aacctttgat agtgtgtggg atgcggtgag
     2161 tgacacccct gaacaagcgg aaaatatgcg gatccgtgcc gagcttgtaa cgatcattaa
     2221 taactggatt gaacaacaag ggtttagcca ggctcaggca gcttctgctt taggggtcac
     2281 tcaacctcgc atctctgaac ttgccagagg taaaatccag atctttagta tcgataaatt
     2341 gatcaccatg atggctcatg caggactaca tattcaacgt attgaagttc aatatccaca
     2401 tgctgcttag tgctataaat acagatggcg acaaattgat gccgccatct atataacttg
     2461 tcttacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc tttattccgc
     2521 tcggcccagt cattcaggcg acgtttcgct tcgtcctgta gatgtttgcg cagtaactga
     2581 tccacttgca gactgtaatt gagttgctgc cagttgccat aaacccgcag cggtactggc
     2641 gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac gacccgaata
     2701 tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc tgtaagcgcc
     2761 agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt caacgtcaaa
     2821 tcggtggtaa agcggtcaag acgcgttacg ttatcgaagt tttcagcggc cttcacatca
     2881 ccaccattac gttctaccgc ttgctgaatc atctgctgga agttcatccc ttccatgcgc
     2941 gtatcggcca tttcgacatg tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct
     3001 atgtcagcac cggagaaatc accagccaat gacatttttc cggtcaacga aatcggatag
     3061 ttaaacgcct tcagaatggt gccaatctca acgttttcca gccgtggctg gaagtttatc
     3121 cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctgtcc accgttaagc
     3181 ttgccctgta gttgagtaat ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca
     3241 tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat atcagccgta
     3301 aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaataac cgggcgcggc
     3361 aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc ggtttcatta
     3421 agcgggatga ggttgtcaag attcagttgc gggaattgca gcctcagctg ccattccggt
     3481 ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tatcattggc ggtcaaacta
     3541 atttggttaa atgaaaggcg tttacgtgac tcctgccact gggcctggaa actcccctgc
     3601 ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat ttgttcaata
     3661 gccgccgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag ggatatcgtg
     3721 agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg atgttgggga
     3781 tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc ttcatgctgg
     3841 aacaccagca cgctgtccgc caccttaaga ctggatatat caaacgacca tccgcgatcg
     3901 tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc
     3961 gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg cttaacgctc
     4021 agttgatgac tcagtagtgg taaaagcgcc acgtccaggc gcatgttgtc ggcgcgaacc
     4081 agtggctggc tcgcgccctg ggcggtgaga gacattcgcc cggagaggat actaagctgc
     4141 ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct acgtgcagca
     4201 acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac taacgcagat
     4261 aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg tctcatggta
     4321 tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc ctgctggttg
     4381 cgatacttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga aagcggctca
     4441 aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt accggagttg
     4501 tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc ggtgacgtgc
     4561 accatcagcc ccagacgcgc cagtgaggaa cgcccgtcca gccagcccac cagatcggct
     4621 ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg aagatagaac
     4681 gcctcgcttt catcgagaac gatctcatcg ctcatcacgc ggtcaagcgc ggcgctcact
     4741 tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat
     4801 ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg
     4861 ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg
     4921 gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg cataccaggt
     4981 tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta ttttcgcttt
     5041 caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt ccgcatattg
     5101 tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat attgcgccat
     5161 cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc gcatcaggca
     5221 gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca aacgtgcatc
     5281 cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc
     5341 tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg agccgcgttt
     5401 caacgcttgt aaatgctcaa gcagcaggct gtgatccatc gcgctgggat ggtcgtagtt
     5461 ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc agtcttcggg
     5521 aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa gggtactggc
     5581 aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc actgatgaga
     5641 ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg ggcgacgaag
     5701 cgccaaacgc ggcaattata gggatttcat ccgcctgata ccagtcgaat agcgttgccg
     5761 cgcgctcaga gttaattgtt gacgaagaat tcccggtggc aaattacgtt gatcagtttt
     5821 atacaaggta aaaattgtta tacgcagttg cgcaaattat ccgcctttac gtcactttat
     5881 gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga aacgaatact
     5941 ggcgacctgg ccttgcggat aaagcggtaa tgagcaaaca atcacagcat gtattaattg
     6001 ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt atcttcaccc
     6061 ttttctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg ttcccgacgt
     6121 ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccaggg attgcgctga
     6181 gctgctcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc tcgctgaaca
     6241 tgacctgcac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat
     6301 tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg gcactcggca
     6361 gcgccattgt tccacctctg ttggggggtg ttctggttgt cctgctgacg cccggagacg
     6421 atcctcatca gggcattttt gatatgggta ctgtcagaat ccatcggcgc tctggcactg
     6481 gtgccgctgg gattgttatt taaaccacac tatctgctgc gccatcgcaa cccacggttg
     6541 ctttttgagt cgctgctcac gttagccatc acactgacgt taagctggct ttcgatgctg
     6601 tatctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg
     6661 ccacgaatgg aagccttttt gatcttcctt accacggtga tgatggtgtc gctgatgatg
     6721 gccgcggatc cctccctgct tgctacgccg cgtacgtacc tgatgagcca tatgccgtgg
     6781 ctaccgtttt tgctgatcct gctgcccgcc aacatcatga cgatggtgat gtatgccttt
     6841 cgtgcggaac gcaaacacat ttccgaaagc gaaacccgtt ttcggaacgc catggaatat
     6901 tccgccatcg gcatggcatt agtgggcacc gagggacaat ggctgcaatc caacaaagca
     6961 ctctgccagt ttctcggtta cagtcaggaa gagctgcgcg gactcacctt tcagcaactg
     7021 acctggccgg aggatctcaa taaagatctc caacaggttg aaaagctgat aagcggtgaa
     7081 ataaacacct attcaatgga aaaacgttac tacaaccgca atggcgatgt tgtctgggcg
     7141 ttgcttgccg tctctctggt gcgccacacg gatggcacgc cgctctattt tatcgctcag
     7201 attgaagaca ttaacgagct aaaacgcacc gaacaggtga atcagcaact gatggaacgc
     7261 atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggaact gaagccgaat
     7321 atttttagct gggataagcg gatgttcgag ctgtatgaaa ttcctccgca tatcaaaccg
     7381 aactggcagg tgtggtacga gtgcgtgctg ccggaagatc gccaacacgc cgaaaaagtg
     7441 attcgtgatt cgttgcaatc acgctcgccc tttaaactgg aatttcgcat tgccgtgaaa
     7501 gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc
     7561 gaacgcctgc tcggcattaa tatggatatg actgaggtta aacagcttaa cgaggcattg
     7621 tttcaggaaa aagagcgcct gcacattacg ctggactcca tcggcgaagc cgtggtctgt
     7681 attgatatgg cgatgaaaat tacctttatg aatccagtgg cggagaagat gagcggctgg
     7741 acgcaggaag aagcgttagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac
     7801 aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc gatcgaacaa
     7861 gatgtggtgt tgcactgtcg gagcggcggc agctacgacg tgcattacag tattacgccg
     7921 ttaagtactc tggacggcag caatataggc tcggtactgg tgattcagga cgttaccgaa
     7981 tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
     8041 gccaaccgcg ccagttttga gaaacaactg cgtatcctgc tgcaaacggt aaacagtacg
     8101 catcagcgac atgcactggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
     8161 gccgggcatg cggcgggcga cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg
     8221 ctgcgctcca gtgacgtgct ggcgcggctc ggcggtgatg aatttggtct gctgctgcca
     8281 gactgcaatg ttgaaagcgc tcgttttatc gctacacgta ttatcagtgc tgtgaatgac
     8341 tatcacttta tatgggaagg ccgggtacat cgggtaggtg ccagcgccgg gattaccttg
     8401 atcgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat
     8461 gcctccaaaa atggtggccg gggccgggtg acggtttacg aaccgcagca agctgccgca
     8521 catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag
     8581 ttgatgatgc tcgcccacgg tgtcgcttcg ccacggatcc cggaagcgcg taatttgtgg
     8641 ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca aacatttcgt
     8701 cgtagcttca gcgatccggc gcttagccat gctcttgacc gccgggtatt ccacgaattt
     8761 ctccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct ccccctttcc
     8821 gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct ggaaaatagc
     8881 cctctaccac cacggttatt acatctgatt attccggcag aagcgatttt cgatcacgca
     8941 gaaagcgtgc aaaaactgcg gctgggggga tgccggatcg tattcagtca ggtgggccgc
     9001 gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt
     9061 gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag
     9121 gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg
     9181 atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgaggtgat tgccgatgcc
     9241 caaccgctgg atttgctgat gaatagtagt tatttcgcga ttaactgagg gagcgtttgc
     9301 ccattgcctg atgcgacgct aacgcgtctt atcaggccta caaatcgctc attccgcaag
     9361 gcgctcgcac cgcatccggc gaccaacgtc atgcttcgtc tggttgccag ccttccgtat
     9421 accagatatg caacagcgca taagaacgcc agggcttcca gcgctcggca taacggcgga
     9481 tttgcgccgg tgtcattccc ggaaatcgct gtttaatcag ataatcatcc ggcaggaaaa
     9541 catctttcgc ctgccagcca cgcaaagcaa aataattcgc cgtccagcgc ccgatacccg
     9601 gaaaagtttg cagcgttttc atcgcctgct ccacatcgcc cggtattgtc attggtaagg
     9661 tgccctccag cgccgcattt gccagatgaa tcagcgcctc tgcccgtttc aacggcatac
     9721 ctaacgcttt taatgcctgc gggtctgctg ctgccagccg ctgaggcgtg gggaagcaga
     9781 tatactccgg aaaatcatcc agccgttcgc cataaagctg taccactttg gcagtcaatt
     9841 ttgccgccat cgccacgctc accagttggc ctaaaatcgc ccgcacgcct tgctcaaaag
     9901 catcaacact gccgggtaaa cgcaatccgg gccgcgccgc gcctaacttg cccaacgcac
     9961 cgttaacaat ctgcgggtta cattgcagat caagcaggcg gctcattttc gccagacact
    10021 cagcggcaac gggttctaaa tctgcactta aatttatgtg cagagtatgg cgggctatat
    10081 ccggaatagc agtcaccacg ccgcgatatt cgcccaccgc cagactacgg gcataataat
    10141 cgtcagcgac cgtttccaca ccactcactg cacgggcggc gagaaatccc aacatccacg
    10201 accagtcata cggcggctgc cagttcaggg tatacatcgc atctccttat tcatccgctt
    10261 tcagcataaa cgttattcag acgctgcgct ttgctttcat attccggttg tcgcgacggc
    10321 aacatttcgc taaagtcacg ccccttcttc accggcatgg ggattatttc gtgtttattg
    10381 gttttgatta cggtacagca aactgttcag tagcggtcat gcgtgacggt aaaccgcact
    10441 tgctaaaaat ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg ccaacgcgtg
    10501 aagcggtaag cgaatggctg taccgccatc atgatgttcc ggcagacgac gatgaaacgc
    10561 aggcactgtt gcgtcgggcg attcgttata atcgcgaaga agatatcgat gttacggcga
    10621 aaagcgtgca gttcggtctt tcctcactgg cacagtacat tgatgatcca gaagaagtgt
    10681 ggtttgtgaa atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg cagcaggtag
    10741 cgctgtttga ggatctggtc tgcgcaatga tgttgcacat tcgccagcag gcgcaagcgc
    10801 agctgccaga agcgattact caggcggtga ttggtcgccc gatcaacttc caggggctgg
    10861 gcggcgatga agcaaacgcc caggcgcaag ggattctgga acgcgcggcg aagcgtgccg
    10921 gattcaggga cgtggtattc cagtacgagc cggtagcggc tgggctggat tacgaagcca
    10981 ccttacagga agaaaaacgg gtactggtgg tggatatcgg cggtggtacg actgactgtt
    11041 cattgctgct gatggggccg cagtggcgtt cgcgtctcga tcgtgaagcc agcctgctgg
    11101 gtcacagtgg ttgccgtatt ggcggtaacg atctggatat cgcactggcg tttaaaaacc
    11161 tgatgccact gctgggtatg ggtggcgaaa ccgaaaaagg catcgccctg ccgattctgc
    11221 cgtggtggaa tgcggttgcc atcaacgacg tacctgcgca gagtgatttc tacagtagtg
    11281 ccaacggtcg tctgcttaac gatctggtac gcgatgcccg cgaaccggaa aaagtggccc
    11341 tgctacagaa agtctggcgt cagcgtttaa gctatcgcct ggtgcgtagc gcagaagagt
    11401 gcaaaattgc gctttcaagc gtagcggaaa cccgcgcctc actgccgttt atcagcgatg
    11461 aactggctac gctgattagc cagcaagggc tggaaagcgc cctcagccag ccactggcgc
    11521 ggattcagga acaggtgcaa ctggcgctgg ataacgccca ggaaaaaccg gacgttatct
    11581 acctgaccgg cggaagcgcc cgttctccgc tgattaaaaa agcgctggca gaacagttgc
    11641 cgggcattcc gattgcaggc ggcgatgact ttggctccgt caccgccggg ctggcacgct
    11701 gggcggaagt ggtgtttcgt taattgaaaa atggctcagc agcggcttaa atctgcctgc
    11761 gcctgagcca gtttttctgc cgcctgacgt aacggttcca tcaacggcat ggtccgtgct
    11821 ggagcatgaa ggcggaatcg ctgcaactct gtcgcgccga cagtcaacgc cgattccagc
    11881 cgatgtcgtt tggcggccat tttcgccatt accgcttgtc tttctgcagg cgttaccgct
    11941 tcgttcggcc tgaacacaaa acggcgttca cagctcgctt tccagtcgat aaccgcctgg
    12001 gtcagatgat caccaaaccc tttaacttgc ttaaccccac gacgggtaac atccgcggct
    12061 gtttcaatac caaaagaccg taacgccgct ttacgcgcag ggccaacgcc gggaatagag
    12121 gcagcatcaa taaaaaatcc ctccagaaac ttctgcttct gccgttccct tgcagtgtca
    12181 tgaagtgctg ccagagcgcg tttctcctct tccggtaacc cgagaatttc gtccttcatt
    12241 ttttcgagca tcgcccgttt ggcgataaaa ccttccagcc cgcccaactg ttggatctgg
    12301 ctgactaaat ggtcatagtc cattttcgcg cgattaaatg cctcacggcg ttgctggatt
    12361 tctgctttat aaactttgct tgtcagactg ccaataatcc agatagccgc cagcacagga
    12421 accaaaataa tataacgcgg ttctgcctga aggccgcaaa gcaacagcga taacgctgac
    12481 agtgcgatct caatcagaat gatgtattcg cgccgtaaca ggcctaaagg aagcggcctg
    12541 ccagtcggtt ggaaatgatc gggtaatggc aattgcaatg ctggcggtgc tactgacgcc
    12601 atcaccatcg cccagacttt cgccagcaca aaatcaccac tggtggtaat gacctcttcg
    12661 ccgagatcaa taaaatagat aacgccttga ttatccagcg cacaccacgg gcagtcagtc
    12721 aaatgaccgg ggtaaacatg cattgccgaa acggtacatt tctttaactg ttggcgtagt
    12781 aaatccagtg ccgctaccca cgcttttgcc gtcggacgcg cggttgccac gccactttcc
    12841 gtgaatgcct gctgaaacat ggcttcaaca tcgcccggta acatcgacag cggaatcgat
    12901 cgtggcggcg gttttaaacc acgtcgctgg ttgtctgacg cgtaggcata acggaaatgg
    12961 gcaatatccg tctccagcgc attacccgca tcagagataa gcggcacacc agaataagga
    13021 tgccgcccac caaacaagac gtgaaaaatc agcaacgcaa ggccaaaatt atcgtgattc
    13081 gcggtacgtt caaaaccgac aaatgatggc aaggtttgta gctctggcgg cgtaaaatgc
    13141 gacacgccga cttcgcataa atgcagtgtg ccattggcgt taatctgaaa ggagtcacta
    13201 tcgatcaaca ccactttgct gtcgcgacct accataaagc tgttctggtt tacgtccccc
    13261 acgacgtgcc cgtgctcgtg aaccgtagca aaagatgaag caatattgcg cgcaacatag
    13321 agtagaaaat cccacgcaca atgaggataa ctctggcgac gatgtgccgg gctatagatc
    13381 atatgaatcg gttctttacc agaaactttt ggcatcataa aaccgataac ttttccgcct
    13441 cgtccaccgt gaagcgttgc ctgcggccag gcgacataat tcaacaactg cgcgtcagct
    13501 gtcgcagcca taaaggcaag tttgtcctgt tttaaggcgg gtggcggcgt gtgataaatc
    13561 ttggcgacgc tatcgacaaa ctcattgata tcataaaccg cgccttcgcc acctttgccc
    13621 agttcacggc ctggcgtaac gcactcacca gtagcagtat ataaagtggg tttcatggta
    13681 accacagcgc cagtgtcaac gttttatcat catcggtacg ctcgtttacc gctgggctcg
    13741 acaaaaactg ctttaacaat tcaggtaata aatcgagttg ttcctgcgtt gctgccgcca
    13801 gtccattgaa aaacggggta aagaaaggca catgcggaga attatccagc atatttaacg
    13861 ccagtcgctg aataccatcc gtaaatgctg caactttatg cgcacgctca gtgctggtga
    13921 aagtttccag tctggaaacg gcatcttcat cggtaatgaa atgcgtcata ttggcatatt
    13981 cacccaccat cggcgtaagc ggtaattgca gaccgtgacc aaaatccacc accacgccgc
    14041 catcgccaat ctgcataatc aaggtgccgt tagccgatga aatcaatccc agaaacgtgc
    14101 aggcaaaatc gcgaactgct aattctttag cttccgcctc ggcaaaaagt cgctgacgaa
    14161 cggtcagcac aatatccgtg gcgagaatat cattgagtcc aaattcaccg tgctgcactt
    14221 tttgcgacac ataagccatc gcttcattga tggcaagcat cgcgccttcg ccaccctgcg
    14281 agacactgcc tgcgccatca gcaaggaaca tcaccagtaa tggctgctgg tcatttagcc
    14341 aggcaacctg catctggcag gcatcctgac aaggtaaatc agcgctaatg tgcgacgttc
    14401 caacagcgga tgcataaacc agacgccagc tcacactgac gtccagcctt ttggcgcttc
    14461 cagcacaact tccgttcccg gcgtggagcg ggaaaccgaa cgcagcgaac tggataacca
    14521 gctaaacagt tcacggaatt gtaatccctg cagtggcaaa ggctgacgaa cgctgatttg
    14581 tgccagagtc ttaatatccg ccccctgaac accgatggaa aagaaggcaa acttcttatc
    14641 ttcttccccc tgaaacactt tgttggcggc agcctgccac tcatcggttg gtgcaccatc
    14701 ggtgatcagg aaaatccacg ggcgataata cgaaatacca tttgcacgat actcacgttt
    14761 ccgctcctct accatatcca gggctttggt aatggcggca cccattggtg tatccccctg
    14821 ggcaaacagg atgggcggga aaaaattagc ggcgctggta aagggttgtt ccacatgcac
    14881 cgggccgaac gtcacaatac caagttccac tctttttaag gccagcgggt cggcaagcag
    14941 ctcatcgcga aaggtaacca atccggcgtt aagttcatta atgggtctac cgttcatcga
    15001 accagaaaca tccagcagca aaatgcaggg gcaacgtggt tccgggttac tggcaaaatc
    15061 gctggtggca aacgtgattt gttcgctcat aagaaatcct ttcttagaga attattgagg
    15121 tccggtatca taccgattgc aataatgaaa taagagcaaa cgaagaaact ttcagaatta
    15181 ggcccggcgg cgcagaaaat tttgctattc gcctcgcaag aacggagagc cgtcgattga
    15241 cactaagggc ggagtgacat aatttcagga gtgagggtta gggaaaggtt tccccctccc
    15301 cctggtgttc ttagtaagcc tggaagctaa tcactaagag tatcaccagt atgatgacgt
    15361 gcttcatcat aacccttttc ttatttaagg ccccttcctc gggaggggct ttcccgtttc
    15421 agcatcccgc tgaaatcgtc ggcttacctc ctttcgccat gcatgcagtc tatcgctgac
    15481 ttgcggataa aatagtttcc agtgtgatta ttgcgtaacg tctcgcaaag aatatttgct
    15541 ggcaggatcg cagactgcaa agcctgcaga ttgacaatct tatcgtgaag gcatactttc
    15601 aggagtgagg gtagagcggg gtttcccccg ccctggtagt cttagtaagc cgggaagctt
    15661 atgactaaga gcaccacgat gatgatccac ttcatcatga ccctttcctt atttaaggcc
    15721 ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct
    15781 ttcgccctga attcagtcta tcgctgactt gcggataaaa tagtttcctc tgtgattatt
    15841 gcgtaacgtc tcgcaaagaa tatttgctgg caggatcgca gactgcaaag cctgcggatt
    15901 gacattctga acgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc
    15961 ctggtagtct tagtatgctg ggtagctaat cactaagagc accacgatga tgagtatctt
    16021 catcatgacc ctttccttat ttatggcccc ttcctcggga gggggctttc ccgtttcagc
    16081 gtcccgctga aatcgtcggc ttacctcctt tcgccatgca agcagtctat cgctaacgcg
    16141 tagataaaat agtttcctgt gttattactg gatgcgtgct cgcaaatgtg cccgccattc
    16201 agacgattcc cgatagtgtt tcataattcc tccatttttc tcccttattg gctggctaca
    16261 ctagtatcat tccgcgaaac gtttcaggaa gagaaactct taacgatgaa aggcagttat
    16321 aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg ccatcgccgc attctggttc
    16381 tggcaaggcc gcaatgactc ccagagtgca gccccagggg cgacgaaaca agcgcagcaa
    16441 tcgccagcgg gtggtcgccg tggtatgcgt tccggcccat tagccccggt tcaggcggcg
    16501 accgccgtag aacaggcagt tccgcgttac ctcaccgggc ttggcaccat taccgctgct
    16561 aatactgtta cggtgcgtag ccgcgtggac ggtcaactga tggcgttaca tttccaggaa
    16621 ggccagcagg tcaaagctgg cgatttactg gcagaaattg accccagcca gttcaaagtt
    16681 gcattagcac aagcccaggg ccaactggca aaagataaag ccacgcttac caacgcccgc
    16741 cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg ccaggagctg
    16801 gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc
    16861 gtcgccagcg cgcagctgca actcgactgg agccgtatta ccgcaccagt cgatggtcgc
    16921 gttggtctca agcaggttga tgttggtaac caaatctcca gtggtgatac caccgggatc
    16981 gtggtgatca cccagacgca tcctatcgat ttgctcttta ccctgccgga aagcgatatc
    17041 gctaccgtcg tgcaggcgca aaaagccgga aaaccgctgg tggtagaagc ctgggatcgc
    17101 accaactcga agaaattaag tgaaggcacg ctgttaagtc tcgataacca aatcgatgcc
    17161 actaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc
    17221 aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtaatccca
    17281 acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac
    17341 aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgc
    17401 gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg
    17461 gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc
    17521 cgcgaatacg cgaaaaaagg agctcgctcc tgatgcaagt gttacccccg agcagcacag
    17581 gcggcccgtc gcgcctgttt attatgcgtc ctgtggccac cacgctgctg atggtggcga
    17641 tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg
    17701 actatccgac cattcaggtg gtcacgctct acccaggtgc gagcccggat gtcatgacct
    17761 ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt
    17821 cgtcgcaaag ttccggcggt gcgtcagtta tcactttaca gttccagcta acattaccgc
    17881 tggatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga
    17941 gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga
    18001 cgctcgccgt cacctcaaca gccatgccga tgacgcaagt ggaagatatg gtggaaaccc
    18061 gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggcc
    18121 agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca
    18181 gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggctaaa ggtagccttg
    18241 acggcccttc ccgtgcggtt acgctttccg ctaacgacca gatgcaatcc gccgaagagt
    18301 atcgccagct aatcatcgcc taccagaacg gcgcgccgat tcgtctgggc gatgtcgcaa
    18361 ctgtagagca aggtgcagaa aacagctggc tcggcgcatg ggcgaataaa gaacaggcca
    18421 ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcatcc
    18481 ggcagatgct gccacagctc accgagagtc tgccgaaatc ggtgaaggtg acggtacttt
    18541 ctgaccgtac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg
    18601 ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca
    18661 ttcccggcgt tgcagtgccg ctgtcgttaa tcggcacctt cgcggtcatg gtgtttctcg
    18721 atttttcaat caataacctg acgctgatgg cgttaactat cgccaccggg ttcgtggtcg
    18781 atgacgccat cgtggtgatc gaaaatattt cccgctatat cgaaaaaggc gaaaaaccgt
    18841 tggcggcggc gctcaaaggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct
    18901 cgctgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc
    18961 gcgaatttgc tattaccctg gcggtagcga ttttaatctc agccgtggtg tcgctaaccc
    19021 tgacaccgat gatgtgcgca cggatgctca gccaggagtc gttgcgtaaa cagaaccgct
    19081 tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg
    19141 cgaaagtgct gaatcatccg tggctgacct taagcgtggc gctcagcacg ctgctgctta
    19201 gcgttctatt gtgggtgttc attccgaaag gtttcttccc ggtacaggat aacggcatta
    19261 ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcggcaac
    19321 gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagtctgacc tcatttgttg
    19381 gcgttgatgg cactaacccg tcgctgaaca gtgcgcgttt gcagatcaac ctcaaaccgt
    19441 tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg gcggtggata
    19501 aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc gatactcagg
    19561 tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct
    19621 gggtgccaca gttgatggaa aaactccagc agctgccaca gatttctgat gtctccagcg
    19681 actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc
    19741 tggggatcag catggcggat gtcgataacg ccctgtacaa cgcgtttggt cagcgactga
    19801 tttccactat ttatactcag gctaaccagt atcgtgtagt gctggagcac aataccgaaa
    19861 ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac ggcggcgtgg
    19921 tgccgctaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc atcaaccatc
    19981 tggatcagtt cccggtaacg accatctcct ttaacgtgcc ggataactat tcgctgggcg
    20041 atgcggtgca ggcaattatg gacaccgaga agacgctgaa tctgccggtg gatatcacca
    20101 cgcaattcca gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga
    20161 ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc
    20221 acccgatcac cattctctcg acgctaccca cggcaggtgt tggcgccctg ctggcgttaa
    20281 tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta
    20341 tcgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag
    20401 gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga
    20461 tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg
    20521 cggaactacg tcgtccgtta ggtatcggca tggtcggcgg cctgattgtc agccaggtgc
    20581 tgacgctgtt caccacgccg gtgatttatc tgctgtttga ccgcctggca ttgtggacca
    20641 aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt
    20701 accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggtt
    20761 tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg
    20821 cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc
    20881 gctcacttgg gcgcattgcc ggggtcagtg aaatgacctc cagcagttcg ctcggcagca
    20941 cgcgtattat tttgcagttt gattttgacc gggatatcaa cggtgcggcg cgtgatgtgc
    21001 aggcggcgat caacgccgca caaagtctgc tgcccagtgg gatgcccagc cgcccgactt
    21061 atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctaacc tccgatactt
    21121 attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa
    21181 tcgacggtgt cggtgatgtc gatgtcggcg gcagctcgct gcccgccgtg cgcgtcgggc
    21241 tgaacccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc actgccatca
    21301 gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga
    21361 tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt attcactaca
    21421 acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gaccgactca gtgcaggatg
    21481 tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaattgc
    21541 cggaagccaa tattatccag acggtagaca gcatccgggc aaaattaccg gagttgcagg
    21601 aaaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg
    21661 cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg
    21721 tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg gtgccggttt
    21781 cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aacctttcgt
    21841 taatggcgct caccatcgct actggtttcg tggtggatga cgccatcgtg gtgctggaaa
    21901 acattgcacg tcatctggaa gcgggaatga aaccgttgca agccgcactg caaggtactc
    21961 gcgaagtcgg ttttacggtg ctgtcgatga gtctgtcgct ggtggcggtg ttcctgccgc
    22021 tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg
    22081 tcgccattgg tatttcgttg ctggtttctc tgacattaac gccaatgatg tgtggctgga
    22141 tgctgaaagc cagcaagccg cgcgagcaaa agcgactgcg tggttttggt cgcatgttgg
    22201 tagccctgca acaaggctat ggtaagtcac tgaaatgggt gctcaatcat acccgtctgg
    22261 tgggcatggt gctgcttggc accattgctt tgaatatctg gctatatatc tcgattccga
    22321 aaaccttctt cccggagcag gacactggcg tgttgatggg cgggatacag gcggatcaga
    22381 gtatttcgtt tcaggcgatg cgcggtaagt tgcaggattt catgaaaatt atccgtgacg
    22441 atccggcagt ggataatgtc accggcttta ccggcggttc gcgagtgaac agcgggatga
    22501 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac agcgcagcaa attattgacc
    22561 gtctacgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacagg
    22621 atattcgcgt tggcggacgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
    22681 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acattgccgg
    22741 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
    22801 atcgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagc ctgttaaata
    22861 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
    22921 tgatggaagt ggatccgcgc tatacccagg acatcagcgc gctggaaaaa atgttcgtaa
    22981 tcaataacga aggaaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
    23041 ccccactatc ggtgaatcat cagggattat cggcagcctc gaccatctcg ttcaacctgc
    23101 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
    23161 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggtgttc caggagacga
    23221 tgaactcaca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
    23281 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
    23341 ttggagcgct gctggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
    23401 ggatcatgct attaatcggc attgtgaaga aaaacgccat tatgatggtc gattttgcgc
    23461 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
    23521 tgcgttttcg cccgattatg atgactaccc tggcggcgct gtttggcgcg ctgccgctgg
    23581 tgttgtcggg cggtgacggc tcggagctgc ggcaaccctt ggggatcacc attgtcggcg
    23641 gtctggtgat gagtcagctc ctgacgctgt ataccacgcc ggtggtatat ctctttttcg
    23701 accgtctgcg gctgcgtttt tcgcgtaaat ctaaacaaac ggtaaccgag taaatgacag
    23761 atcttcccga cagcacccgc tggcaattgt ggattgtggc tttcggcttc tttatgcagt
    23821 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
    23881 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
    23941 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgctatcg
    24001 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
    24061 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
    24121 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
    24181 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtggag tacgcgtcgt
    24241 ggcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga
    24301 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
    24361 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
    24421 cgccgctggc gattactggc ctggtcgcag ttggtgtggt ggcactggtg ctttatctgc
    24481 tgcacgccag aaataacaac cgcgctcttt tcagtctgaa actgttccgc actcgtacct
    24541 tttcgctggg cctggcgggg agctttgccg gacgtatcgg tagcggcatg ttgcccttta
    24601 tgacgccggt tttcctgcaa attggccttg gcttctcacc gtttcatgcc ggactgatga
    24661 tgatcccgat ggtgcttggc agcatgggga tgaagcggat tgttgtacag gtggtgaatc
    24721 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
    24781 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
    24841 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
    24901 cggacaatct ggcgagcagc ggcaacagtc tgctgtcgat gattatgcaa ttgtcgatga
    24961 gtatcggcgt caccatcgcc gggctgttgc tgggactttt tggttcgcag catgtcagcg
    25021 tcgacagcag caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
    25081 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
    25141 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cctggtatta ccggcaaact
    25201 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
    25261 tatcagtttt gagcgtggct ttattgatta catcaagcat ggtaatgaac agcgattaca
    25321 actgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggcaactggc gcttcctgcg
    25381 caacaatgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    25441 taaacccggc ccgggtatgc caccacacgg ctggcgtact cagttctggg tggttgatca
    25501 aaacaacaag gtgctggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    25561 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    25621 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgattgtcgc
    25681 cctggcaacg ttactcgcgg cactcgctac ttttctgctg gcgcgcggtt tgctggcacc
    25741 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    25801 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    25861 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    25921 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    25981 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    26041 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    26101 ggtagatttg atcccactgc tggaagtggc aggcggcgca tttcgcgaac gatttgccag
    26161 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    26221 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    26281 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    26341 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    26401 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    26461 cattgttgaa gcacataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    26521 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    26581 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct gggacagttg
    26641 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    26701 gtactgtcgt acgtgcgcca gacgccgccg gatctgatcc tgttggatct gatgctccct
    26761 ggcaccgatg gcctgacgct gtgccgggaa attcgtcgtt tttctgacat tcctatcgta
    26821 atggtgacag caaaaatcga agagatcgac cgcctgctag ggctggagat tggcgcagat
    26881 gactacattt gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    26941 cgccgttgca aaccgcagcg tgagttgcag caacaggatg ctgaaagccc gttgattatc
    27001 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    27061 tttcgtctgc tgaaaacgct ctctcatgaa ccaggaaaag tgttctcccg cgagcaattg
    27121 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gtaccatcga cagccacatt
    27181 aaaaacctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    27241 tacggcgtcg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    27301 tactttgtta gccggagatg gcattcagac cagatccggc aacattatcc cacgcatggt
    27361 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    27421 cggtaaacag cacctttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat
    27481 ggctggttgg tttgaactca gtaagagcag tgatagtcag ttccggtttg tgctaaaagc
    27541 gggcaatggt gagacgatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    27601 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc
    27661 aagcaacggc aaattctatt tcaatctgaa agccgccaat catcaaatta tcggctccag
    27721 ccagatgtat gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    27781 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    27841 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcac
    27901 tcccctaacc gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat
    27961 gtttaaaccg gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc
    28021 ttatggcgca gatgctgttt atgcgggcca gccgcgttac tccctgcgtg tgcgcaacaa
    28081 cgaattcaac cacgaaaacc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa
    28141 gttttatgtc gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga
    28201 cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat
    28261 tatgctggtg cgtgagcact tccctgaaat gccgatccat ctctcggtac aggctaacgc
    28321 cgtgaactgg gcgacggtga aattctggca gcaaatggga ctgacccgcg tgatcctctc
    28381 tcgcgagctg tcgctggaag agattgaaga gatccgcaat caggtgccgg atatggagat
    28441 cgagatcttc gttcacggcg cactgtgcat ggcctactcc ggtcgctgcc tgctctctgg
    28501 ctatatcaac aagcgcgatc cgaaccaggg tacctgcacc aacgcctgcc gctgggagta
    28561 caacgtccag gaagggaaag aagatgacgt tggcaacatc gtacacaagt acgagccgat
    28621 tccggtgcaa aatgttgagc cgacgctggg tatcggcgcg ccaaccgaca aagtgtttat
    28681 gatcgaagaa gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac
    28741 ttacatcatg aactcgaaag atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat
    28801 gggcgtgcat tcgctgaaaa tcgaaggccg tactaaatct ttctactatt gcgcacgcac
    28861 cgcacaggtt tatcgtaaag ctatcgatga tgccgctgcg ggcaaaccgt tcgataccag
    28921 cctgctggaa actctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg
    28981 tcatactcac gacgattatc agaactacga atacggttat tcagtttctg accgccagca
    29041 gtttgttggt gagtttaccg gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa
    29101 taaattctcc gttggcgaca gccttgagct gatgacgccg caaggcaaca ttaactttac
    29161 ccttgagcac atggaaaacg ccaaaggcga agctatgccg gtagcaccag gcgatggtta
    29221 tactgtgtgg ctcccggtgc cgcaggatct tgagctaaat tacgcgctgc tgatgcgtaa
    29281 tttctccggg gaaactacgc gtaaccccca cggtaagtga ttaatttcga ttatttttcc
    29341 cggatggaaa attcttagaa accgatcaca tacagctgca tttattaagg ttatcatccg
    29401 tttcgctgaa aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct
    29461 gtgtaatctc ccttacacgg gcttattttt tatgcataag ccctatccct gataaccgtc
    29521 ttccattgac cacatcgata gaatctccct tcatagcacg atgcctttca cgtaacggca
    29581 tcgtgctcgc acaggttccg gctaagcaca accagaacgc gcatgtttga cgcttaccaa
    29641 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    29701 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    29761 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    29821 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    29881 tttaccgata cttgtctcat cattatgcac agcacaacca gacataagaa aaacactaat
    29941 taatgatata gcagctatct ttttcatctc accttccccc attaaat
//
LOCUS       DAZGXS010000048        29916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGXS010000048 DAZGXS010000000
VERSION     DAZGXS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..586)
                     /gene="yncG"
                     /locus_tag="ACHKG5_002246"
     CDS             complement(<1..586)
                     /gene="yncG"
                     /locus_tag="ACHKG5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0868349.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTL"
     gene            853..2352
                     /gene="ansP"
                     /locus_tag="ACHKG5_002247"
     CDS             853..2352
                     /gene="ansP"
                     /locus_tag="ACHKG5_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP0868350.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2467..3528)
                     /gene="yncE"
                     /locus_tag="ACHKG5_002248"
     CDS             complement(2467..3528)
                     /gene="yncE"
                     /locus_tag="ACHKG5_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0868351.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3770..5872
                     /gene="pqqU"
                     /locus_tag="ACHKG5_002249"
     CDS             3770..5872
                     /gene="pqqU"
                     /locus_tag="ACHKG5_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0868352.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5908..6573)
                     /gene="mcbR"
                     /locus_tag="ACHKG5_002250"
     CDS             complement(5908..6573)
                     /gene="mcbR"
                     /locus_tag="ACHKG5_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0868353.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6771..7808)
                     /gene="curA"
                     /locus_tag="ACHKG5_002251"
     CDS             complement(6771..7808)
                     /gene="curA"
                     /locus_tag="ACHKG5_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0868354.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7989..8507
                     /gene="mddA"
                     /locus_tag="ACHKG5_002252"
     CDS             7989..8507
                     /gene="mddA"
                     /locus_tag="ACHKG5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0868355.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8504..8953
                     /gene="ydcZ"
                     /locus_tag="ACHKG5_002253"
     CDS             8504..8953
                     /gene="ydcZ"
                     /locus_tag="ACHKG5_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0868356.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8954..9187)
                     /gene="ydcY"
                     /locus_tag="ACHKG5_002254"
     CDS             complement(8954..9187)
                     /gene="ydcY"
                     /locus_tag="ACHKG5_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0868357.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9273..9446)
                     /gene="ortT"
                     /locus_tag="ACHKG5_002255"
     CDS             complement(9273..9446)
                     /gene="ortT"
                     /locus_tag="ACHKG5_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0868358.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9641..9736
                     /gene="yncL"
                     /locus_tag="ACHKG5_002256"
     CDS             9641..9736
                     /gene="yncL"
                     /locus_tag="ACHKG5_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0868359.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9833..11257)
                     /gene="patD"
                     /locus_tag="ACHKG5_002257"
     CDS             complement(9833..11257)
                     /gene="patD"
                     /locus_tag="ACHKG5_002257"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0868360.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11279..12073)
                     /gene="ydcV"
                     /locus_tag="ACHKG5_002258"
     CDS             complement(11279..12073)
                     /gene="ydcV"
                     /locus_tag="ACHKG5_002258"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868361.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12063..13004)
                     /gene="ydcU"
                     /locus_tag="ACHKG5_002259"
     CDS             complement(12063..13004)
                     /gene="ydcU"
                     /locus_tag="ACHKG5_002259"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868362.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13005..14018)
                     /gene="ydcT"
                     /locus_tag="ACHKG5_002260"
     CDS             complement(13005..14018)
                     /gene="ydcT"
                     /locus_tag="ACHKG5_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0868363.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14036..15181)
                     /gene="ydcS"
                     /locus_tag="ACHKG5_002261"
     CDS             complement(14036..15181)
                     /gene="ydcS"
                     /locus_tag="ACHKG5_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0868364.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15426..16832)
                     /gene="ydcR"
                     /locus_tag="ACHKG5_002262"
     CDS             complement(15426..16832)
                     /gene="ydcR"
                     /locus_tag="ACHKG5_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0868365.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16911..17327)
                     /gene="hicB"
                     /locus_tag="ACHKG5_002263"
     CDS             complement(16911..17327)
                     /gene="hicB"
                     /locus_tag="ACHKG5_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0868366.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17373..17549)
                     /gene="hicA"
                     /locus_tag="ACHKG5_002264"
     CDS             complement(17373..17549)
                     /gene="hicA"
                     /locus_tag="ACHKG5_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0868367.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17771..18001
                     /gene="yncJ"
                     /locus_tag="ACHKG5_002265"
     CDS             17771..18001
                     /gene="yncJ"
                     /locus_tag="ACHKG5_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP0868368.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18093..20054)
                     /gene="rlhA"
                     /locus_tag="ACHKG5_002266"
     CDS             complement(18093..20054)
                     /gene="rlhA"
                     /locus_tag="ACHKG5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0868369.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20127..20663)
                     /gene="sutR"
                     /locus_tag="ACHKG5_002267"
     CDS             complement(20127..20663)
                     /gene="sutR"
                     /locus_tag="ACHKG5_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0868370.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20716..21927
                     /gene="ydcO"
                     /locus_tag="ACHKG5_002268"
     CDS             20716..21927
                     /gene="ydcO"
                     /locus_tag="ACHKG5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0868371.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(21962..22630)
                     /gene="ydcL"
                     /locus_tag="ACHKG5_002269"
     CDS             complement(21962..22630)
                     /gene="ydcL"
                     /locus_tag="ACHKG5_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0868372.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22934..23527)
                     /gene="tehB"
                     /locus_tag="ACHKG5_002270"
     CDS             complement(22934..23527)
                     /gene="tehB"
                     /locus_tag="ACHKG5_002270"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0868373.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23524..24516)
                     /gene="tehA"
                     /locus_tag="ACHKG5_002271"
     CDS             complement(23524..24516)
                     /gene="tehA"
                     /locus_tag="ACHKG5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0868374.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24640..25620
                     /gene="ydcK"
                     /locus_tag="ACHKG5_002272"
     CDS             24640..25620
                     /gene="ydcK"
                     /locus_tag="ACHKG5_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP0868375.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25615..26151)
                     /gene="rimL"
                     /locus_tag="ACHKG5_002273"
                     /pseudo
     CDS             complement(25615..26151)
                     /gene="rimL"
                     /locus_tag="ACHKG5_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26214..26438)
                     /gene="ydcH"
                     /locus_tag="ACHKG5_002274"
     CDS             complement(26214..26438)
                     /gene="ydcH"
                     /locus_tag="ACHKG5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0868376.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26578..28233)
                     /gene="opgD"
                     /locus_tag="ACHKG5_002275"
                     /gene_synonym="mdoD"
     CDS             complement(26578..28233)
                     /gene="opgD"
                     /locus_tag="ACHKG5_002275"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0868377.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(28458..29801)
                     /gene="ydcJ"
                     /locus_tag="ACHKG5_002276"
     CDS             complement(28458..29801)
                     /gene="ydcJ"
                     /locus_tag="ACHKG5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0868378.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         7400 a         7652 c         7281 g         7583 t
ORIGIN      
        1 gtaacgttgg taactgacat actgcatcag caatcgcact gatattcgtg gcattatcct
       61 gaaaccattc atgcccaggc ccccatgtgc gcatagtgca aagataacag tccaccagcg
      121 ttagttgctc accaaacgca taaggttcag cggtcaattg tgaattcagc cagacataaa
      181 gcgatttacg atattcaata acattcttct ttaactgttc aggtgcgtca gatgcccaac
      241 gctcagggta atcggcgaaa gtgaacgttg gatagacatt agcaaccagc cagaccaata
      301 accgttgaaa ctgctgacgt tcggcacgcc caaccggtgg cgcaaggtcc gggcgacgat
      361 cgagaaccat caatgcaatc gccgctgtct ccgtcataat ctcgtcattt tccagtgcca
      421 gtgtcggaac ctgacacaac gggttcaggg ttttcaataa ctcgcgtgaa gttccctcgt
      481 ggtcaaaacc actgacatca acgaactgat aaggaatatc agccagtgtt agcatcagct
      541 cactgattgt agagccccat ccgggtacgc catacacctt aatcatatgc ccctcacaat
      601 cttttaaaga ttaaatgtag gacagtacaa aggttaacca gcaaaaggat ttgtcatttt
      661 ttcgtcagtt gtattaatcc ccttcaggat tgtaagggga tgttttgcat cgtttatcga
      721 taacgggtag gatgctgcgc cgatagtttt atacccataa agaataatgg tgataactat
      781 catcgccagg atgaataaac attgttcatg gcaacttata tgactttttc attaaagcaa
      841 tcagggagag caatgagtaa acacgacacc gacacttcag atcaacacgc cgcgaaacgc
      901 cgctggctta atgcccacga agaggggtat cacaaagcga tgggcaatcg ccaggtacag
      961 atgatcgcca ttggcggcgc gattggcacc ggcttgtttt taggtgcagg agcccgactg
     1021 caaatggcgg gtccagcact ggcactggtt tatttaattt gtggcttgtt ttcgtttttt
     1081 attctgcgtg cattgggtga gctggtgcta caccgccctt ccagtggcag ttttgtctct
     1141 tatgcccgtg agtttttggg tgagaaagcc gcttatgttg ctggctggat gtacttcatc
     1201 aactgggcga tgacggggat tgttgatatt accgccgtcg ctctgtatat gcattactgg
     1261 ggtgcgtttg gcggcgtgcc gcagtgggtc tttgcgctcg ctgcacttac catcgttggc
     1321 accatgaata tgatcggtgt gaaatggttt gcggagatgg agttctggtt tgcgcttatt
     1381 aaagtgctcg ccattgtgac ctttttggtc gtgggtacgg tgttcctcgg tagtggtcag
     1441 ccgctggatg gcaacaccac tggctttcat ttaatcaccg ataatggcgg cttcttcccc
     1501 cacggtttgc tgcctgcgct ggtgttgatt cagggcgtag tgtttgcttt tgcctccatt
     1561 gaaatggtgg gtacagctgc cggagaatgt aaagatccgc agaccatggt gcctaaagcg
     1621 attaacagtg tgatttggcg tattggcctg ttttacgtcg gctccgtggt gttgctggtt
     1681 atgttattgc cgtggagcgc gtatcaggcg gggcaaagtc cgttcgtgac gtttttctct
     1741 aaactgggtg tgccatatat cggcagcatt atgaacattg tggtgctgac cgctgccctc
     1801 tccagcctga attcaggtct gtactgcacc ggacgtattc tgcgctcaat ggcgatgggc
     1861 ggttccgcac cgagttttat ggcgaaaatg agtcgtcagc atgtgccgta tgccgggatt
     1921 ctggcgacac tggttgtgta tgtcgtcggc gtattcctca actatctggt gccgtcgcgc
     1981 gtatttgaga ttgtgttgaa cttcgcgtcg ctgggaatca tcgcttcatg ggcgtttatc
     2041 atcgtgtgcc agatgcgcct gcgtaaagcg attaaagaag gcaaagcagc ggatgtcagt
     2101 tttaaactgc ctggcgcgcc cttcacttcc tggctgacat tactgttttt actgagtgtc
     2161 cttgtgctga tggcgttcga ttacccgaac gggacttata ctatcgcggc gctgccgatt
     2221 atcggtattc tgctggttat aggctggttt ggtgtgcgca aacgcgttgc tgaaattcac
     2281 agcactgcgc cagtcgtcga ggaagatgaa gaaaaacagg aaattgtgtt taagcctgaa
     2341 acggcgagct aattgcaaaa ttgcccggct acgtgccggg caaatttaga ttactgacga
     2401 aggtgtgccg catgcggcgt gatcaaagtc aggcaagtct ctccgttatc accctcgcca
     2461 tatctattac agcgcaatac gaatcacatc gtctggctgg gtagcttcct gctgtttagt
     2521 ggatttttgt ttcacactga catacagcgt tttgccatcg gcagacagcg ccaggctgtt
     2581 cggatgagtc ggcgtatcga acgttttcac cactttatag cttttcgcgt caatcacact
     2641 gactttacct gcctgacgat gcgttacgta ggcttcatta cgcgctgggt taaacagcac
     2701 agccagtgat tccggtgccg caaccttcgc cagaatattg ccattacggg tatcgaccac
     2761 taacacttct gccgctttag aatcggtgat aaatgcacgc tgcctggcgg tatcaaggct
     2821 gatgttgata aagaagtgct ctttgccgtc atccagcagc tttttacggc tgaggatttt
     2881 attgtcggcg gtgtcgatgg taatcaattc gccgtcagcg ttagtggtgt aaagacgttt
     2941 gcctttgcta tccagtgcca gaccggtact cattttaccg gtgttctgga tggcggtttt
     3001 cagtttgata ttctcgccat caacgaccca aatcacgcta tctttaccaa taccactgat
     3061 gtaaacggtg ttcgtggcat catcagctac cagctcacgc ggttgcagcg ggcgcacctc
     3121 ttccgtgcgc ttacgatcat ccagcaccag acggcctttc acctcgccag ttttggcatc
     3181 tatcgccgtg accgcgctgt ttacggtgtt accaaaccac aacgtctgag tcgtgttatt
     3241 gatggtggca ccaaacggct tgagatcgtt atggatcgcc tgcgtcactt ccagagtgac
     3301 cggatcaaga cgataaacca cgccgccttt atccagtttg cggctttgcg aagtggcgag
     3361 ccacagcgcg ttttcttgct ggctataagc catttcgtag gcacctttac ctaccgcttt
     3421 acgcagcatt tcttctgcgg cctgcgtact gaatgatgaa gcaacaagca atgaacctaa
     3481 cagtaatgaa ccacgcaggc gcaatgaaaa cagatgacgt aaatgcatga cgactccctt
     3541 tgatgaaccg atatttttgc tgcttcacaa tgcttcgcta agcgtaatca tcgttacgct
     3601 cttgttataa atgggaatgg taatcattat ttactcaaat gtggagcatt tcttgccgtt
     3661 acgactacaa ttctctcaat taattaacgt atcgtgccgt ttcgttttcc ataaatttac
     3721 aatttcatta ccatgacggt gttttcccat tcttaacctg gcttttttca tgaagattgt
     3781 tttcgttcga cagaccgttt tgcccgccct gcttgtcctt tcccccgttg tttttgccgc
     3841 tgatgaacag accatgattg tcagtgccgc gccgcaggtg gtttcagaac tggatacccc
     3901 tgcagcagta agcgtggtgg atggcgagga gatgcgcctg gcaacaccgc gcattaactt
     3961 gtccgaatca ctgaccggcg tgcctggttt gcaggtacaa aaccggcaga actatgcgca
     4021 agatttacag ctgtcgattc gtggatttgg ctcccgctcc acttacggaa ttcgcggtat
     4081 tcgcctgtat gtggacggta ttcccgctac catgcccgac gggcaagggc aaacatccaa
     4141 catcgattta agcagtgtgc aaaatgtgga agtgctgcgt ggccccttct ctgccctgta
     4201 tggcaacgcg tctggcggtg taatgaatgt caccacccag accgggcaac agccaccaac
     4261 cattgaagcc agtagttact acggcagttt tggcagctgg cgctatgggc tgaaagcaac
     4321 gggcgcaacg ggagacggca cacagcctgg cgatgttgat tacaccgtct caaccacgcg
     4381 ttttacgacc cacggctatc gtgaccatag tggcgcacag aaaaatttag ctaatgccaa
     4441 actgggcgta cgcattgatg acgccagcaa attaagcctg attttcaata gcgtggatat
     4501 caaagcagat gacccaggtg ggctaaccaa agcagaatgg aaagcgaatc cgcaacaagc
     4561 gcctcgtgca gaacagtacg acacgcgaaa aaccatcaag caaactcagg ctgggttgcg
     4621 ctatgaacgt agcctgagtt cgcgggatga tatgagtgtg atgatgtatg ccggagagcg
     4681 agaaacgacc cagtaccagt caatacccat ggcaccacaa cttaacccgt cacatgcggg
     4741 cagcgtgatt accctgcaac gccattacca gggaatagac agccgctgga cacaccgtgg
     4801 tgaactgggc gttccggtca cgttcactac cggcctgaac tacgaaaaca tgagtgaaaa
     4861 ccgcaagggc tacaataact tccgcctgaa tagcggcatg ccggagtacg ggcaaaaagg
     4921 tgagttgcgt cgcgacgaac gcaatctgat gtggaacatc gatccctatt tacagacgca
     4981 gtggcagctg agcgaaaaac tgtcgctgga tgctggcgtg cgctacagct ccgtgtggtt
     5041 tgattccaac gaccattacg ttactccggg taacggcgat gacagcggtg atgccagtta
     5101 tcacaaatgg ctaccagccg gatcgttaaa atatgcaatg accgatgcct ggaatatcta
     5161 tctggcagcc gggcgtggtt ttgaaacgcc gacgattaat gagctgtctt atcgcgctga
     5221 tgggcaaagc ggtatgaact ttggtttaaa accatccacc aacgatacaa ttgagatcgg
     5281 cagtaaaacg cgtattggtg atgggctgct tagtctcgca ttgttccaga ccgacaccga
     5341 tgatgaaatt gttgtcgata gcagtagcgg tgggcgtacg acttacaaaa atgctggaaa
     5401 gacccgtcgt caaggcgctg aactggcatg ggatcaacgt ttcgcgggag attttcgcgt
     5461 aaacgcgtcc tggacctggc ttgatgcgac ctatcgcagc aatgtttgca acgaacagga
     5521 ttgtaacggt aatcggatgc cagggatcgc ccgtaatatg ggctttgcgt cgataggtta
     5581 tgtaccggaa gatggttggt atgcaggcat ggaagcgcgt tatatgggcg atattatggc
     5641 agatgatgaa aatacggcca aagcgccgtc ttatactctc gtcggcttat tcaccgggta
     5701 taaatacaat taccacaatt taactgtgga tttgtttggt cgtgtcgata atttattcga
     5761 taaagaatac gttggttctg tcattgtcaa tgagtcaaac gggcgatatt acgaacctgc
     5821 gcccgggcga aattatggtg tcggcgtgaa tatcgcatgg cgatttgagt aattaccttg
     5881 atgcccggta tttgccgggc atttacttta acgattgtat tgctggtata aaatagcaat
     5941 attttgttgc ataatttcag caagacaatg tctgcatccc tctttgtctt ttgcttttaa
     6001 cgcagcgagt aacagatgat agttttctat atgctcccgt aattccgctg gattaatcgc
     6061 ttcatagaga taatgtaaac caggtcccat cctgacccac agttgctcaa tcatctcaca
     6121 caggatgggc atatttgagc gatgataaat cgctaagcga aatagcctgt ttacattaat
     6181 gatttgttcc atgtcgccct tttcttgcgc ctgctgtaat ttctcgagca attcctggag
     6241 ttccgccagg tcttgcgggg tgaggttttc aacagccaga gcaactgcca ttaattccag
     6301 ttcgtagcgg atccgattga tttcgtctaa ttgacgtttc cccacttccg gaactgtaaa
     6361 tgcttgcgct ggtgcgacag aaagcgcatt caccgaaacc agacgtaata atgcttcacg
     6421 cacaggtgta atactcatac ctaattgctc cgccagattt ttagtaatca ggcgtgcgcc
     6481 aggttttagt gcgccaatac taagctgatg tttcaggtcg ttctcaacct gcagagtcaa
     6541 actgacatgt ttcatttttt ccgttccagg cattttaatt ccttcgcgtg ttttctgtcc
     6601 aaccctgcct gcatggcaga aggaattcac ctggtgtatt aaagtgaata atcataaacc
     6661 actcaccttt gttgttatta gcagcagaac aatgatgtgg ttagttaatt aaagctgaaa
     6721 ataagctttt tgcctggatt tttatggcgg cttacgccgc cgttgaaact ttaatcatca
     6781 cccgccacgc ggatcaccac tttgccgaag tttttaccct tcagcaggcc gataaacgtc
     6841 tgtggcgcat tctctaaacc gtcagtaatt tcttcgcggt agtggatttt atcctctttc
     6901 acccattgcc ccatctccct ctgaaactca tggatgcggt gaccataatc ctgagcgata
     6961 ataaaacctt gcaagcgaat acgttttttc agcactgtag ccatcaacag aggtaaacga
     7021 tccggaccgg gtggtagctc tgtagcgtta tagctgctca ctaatccgca gacgggaatg
     7081 cgcgcagatg tattaagtaa cggtagcacc gcatcgaata ccttaccgcc cacgttttca
     7141 taatagatat caataccttt tgggcacgct ttcgccagtt gttcggcaaa atcatccgcg
     7201 tggtgatcaa gacaaacatc gaagcctaac acctcggtag catggcggca tttttccgcg
     7261 ccaccggcta cccccaccac tctgcaacct ttaagtttgc cgatttgccc caccgtcgcc
     7321 cccactggtc ctgtcgccgc agctaccacc aacgtttcgc cctctttagg ctgaccgata
     7381 tccagtaggc ccatataagc ggtaaagcct ggcatcccta gcacacccag cgaccacgat
     7441 ggattttgcg gatgatcgcc aagtttcacc agatcatcac cactggatat gtcatagtct
     7501 tgccatccac tgtagcccag cacccagtcg ccagactgat aatcaggatg attcgactcc
     7561 acgacacggc tcaccgtacc gccgaccatc acgccgccaa tatcaaccgg cggtgaataa
     7621 gatggctcat cgctcatacg tccacgcata tacgggtcca gggacaaata aactgtgcgc
     7681 agtaacacct gtccttcacc cggtgtggcg acatcatctt cttcaagacg gaaattctcc
     7741 ggaaccggtg cgccatgtgg acgcgaggcc agaacccaac gtcgattacg ctgcttttgt
     7801 tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc aggctcgcag
     7861 gattggtttt gtttgcagcc gtcacccccc ctctgcaaca cgcaataatt tcatcgagga
     7921 atccgttgct tcgggctgtt ttcgatagta ttgataacat catcatcatt attttattga
     7981 ggccagacat gtccatccgt tttgcccgca aagccgactg tgctgccatt gcggaaattt
     8041 ataaccacgc cgtgttgtat acggcggcta tctggaatga ccaaacggtg gatgcagata
     8101 accgcattgc ctggtttgaa gcgcggacca tagcaggtta tccggtgctg gtaagtgaag
     8161 aagatggcgt agtgacggga tatgcctcat ttggcgactg gcgtagtttc gatggttttc
     8221 gccataccgt ggaacattcg gtttatgtcc atcccgatca tcagggcaaa ggtctggggc
     8281 gtaaattgtt aagccgattg attgatgaag cgcgagattg cgggaagcat gtcatggtcg
     8341 ccgggatcga atcgcaaaat caggcctcgc tgcatctgca ccagtcgctg ggatttgtcg
     8401 tcaccgcaca aatgccgcag gtgggcacta aatttgggcg ctggctggat ctgacattta
     8461 tgcagttgca actcgacgaa cgcactgaac cggacgcgat tggatgaatc agtcgctcac
     8521 ccttgccttt ttgattgccg ccggaattgg tctggtagtc cagaacacgc tgatggtgcg
     8581 catcacccag acctcctcta ccattctcat cgccatgttg ctgaactcac tggtggggat
     8641 tgtgctcttt gtttctattt tatggtttaa acaaggtatg gcggggtttg gcgaactggt
     8701 gtccagcgtg cgctggtgga cacttatacc tggcttgctg ggatcgtttt ttgtcttcgc
     8761 cagtatcagc ggatatcaga atgtgggagc ggcgacaact attgcagtac tggtcgccag
     8821 ccagctgatt ggcggtctgg ttctggatat cttccgcagt catggcgtac cactacgggc
     8881 gttgttcggc ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggt tggtagccag
     8941 acgctcattt taatcacagg atggtgccgc ctttggttaa ctgttcatgg cgcgcttcca
     9001 tatcttcttt atgcttgcga ccgtgatgag caatagcggt gcgcaaacgc tgctgctggg
     9061 tgtagcgatc ttcccgactt aactccgcgt catcgctcag ggcgattaat aattcgttca
     9121 tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact tcatccagat
     9181 gcgacatact ttctccttag ccacttgtga aatttatcga ttcatccaaa tgatataagt
     9241 ctgcgtggtg ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa cagcgccacc
     9301 gggaaactca tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg gatgcgtttc
     9361 ttatcgtgag aaagaaacca agtgataaga aatgcgattg ctgccataac cgcataaaaa
     9421 acaagcatgt gttgatagag agacatgtac gggcgtccag ccagatgaat aaaacgcgcg
     9481 caaatatgcg tcattttttg acgtgcatca agttttttaa ccggtttgag aaaaaaacat
     9541 caaacagcaa cttgccctta tccatcttca gaatgagtat tattgcggat tttcttaacc
     9601 cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt ccagtagaac
     9661 tgtagtactg ataaatgtct ttgctgctgt tggtttgttt actcttatct ctatgagatt
     9721 tggctggttt atttgatgta gtgattttgc acgcagcacg tactgtcaga gaaatcagtg
     9781 ctctgacagt acgcaagatc atctcatact cttacgatct gcaatattat ccttaatgtt
     9841 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     9901 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     9961 tgacccaggt acaaccatat tgcagccgtg cactgacgcg atgcgccctg cccacatctt
    10021 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttt accacctgtt
    10081 cttcgttgtc gaagagcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
    10141 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
    10201 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
    10261 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
    10321 ccggcgcacc agattttaac gttgccactg cagcaccgag tttttccacc agcgtatcgt
    10381 aaatgccttt ttgcgcgtag atccgacaag ccacagtaca atcctgtccg gcattgtaat
    10441 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
    10501 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggagggcgcg gtatggctga
    10561 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
    10621 cggtcagcgg atcgcccacc gttttgcctc tgccaaacag tacgttaatc acgcctgccg
    10681 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
    10741 gtttaagcac tacgcagttc cctgccgcca gcgccggagc cagtttccac gctgccatca
    10801 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
    10861 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
    10921 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
    10981 gcggtttgcc acaattacgg gactccagtt cggcaaaaac ctgaccattt tcttcgataa
    11041 catcagccag tttcagcaga cattccgcac gcactttcgg cgtggtttgc ccccattcgg
    11101 caaatgctgc atctgccgcg cgcacagcag catcgacctg ctctgcggat gcctcggcaa
    11161 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
    11221 taaccagttc tccgttaatc agtaacttat gttgcatagc atattcctgt atcagtggtt
    11281 attgaccatt gtcgccttcg cgggttagcc accaggctcc caggatcggc aaggttgtta
    11341 ccaacataac cagcagtgcc accacgttag ttaccggtac atcacgcggt cgcccaagct
    11401 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
    11461 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
    11521 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
    11581 caaccagact ccatgaggtg cggcgaaaac gggcgatgac attgttaaac accacaacta
    11641 cacaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
    11701 taaaggcggt taataacgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
    11761 gtaacagcga aatggcgttt ttgccgaaaa agtctcgtcg ccacagcgcg gcagctgcca
    11821 gcgtccctaa caccagcgcc attaatgtcg ccagcgccgc cactttaagt gacagtgtca
    11881 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
    11941 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
    12001 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
    12061 gttcagagtg catcaaacgc tcccagacgt ttcacgaacg ccaggtacag tgcgataaga
    12121 acaatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
    12181 gaataaacca tattgccgat aaaatatcct ggcgggccaa ccagctgcgg gacgataaaa
    12241 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gatcgccagc
    12301 ggcaacacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc tgacgcctgc
    12361 aacaatgacg gcggcaaacg ctcaagcgcc gcctgcacgg gcaggatcat gaacggcagc
    12421 cagatataga gaaacaccag aaagcgcccc agcccggaag ttgacagcgt attgccgccc
    12481 accgcaggta atgtaaggaa cgctgtcagc agtggttcca gcccaagatg ttgtaaaaac
    12541 cactgagcca cgccatcttt tgccagcaat aacgtccagg cataggcttt aacaatgtag
    12601 ctcgcccaca tcggcaacat taccgcaata taaaaaaacg ctttcatttt cccgctggta
    12661 tagcgcgcca tataccacgc cattggaaaa gccagaatgg cgctggcgat agtgactgcc
    12721 acagccatag tcagcgtgcg gagaatgatg tcgtaattcg ccggattaaa cagcgcacgg
    12781 atgttcgcca gcgtcagttc cggcgttacc gacatggtga aatcgtcaaa ggtataaaag
    12841 ccctgccata acagcgttag cagcgagccg aaatagacaa tgccaaacca cattagcggg
    12901 ccaagcagca gcaaaaacag ccccagcccc ggattccgcc agaagaaacc ggacacctta
    12961 cccagacctg gacgtgaagg tgattgcaat acgttcatcg ccattcacct ctcctcaacc
    13021 agcggcacca tcacatcacg cgaccaggaa accatcacct gttgtccggg cgtgagcgtg
    13081 gcaggcagtt cttcgcctgt catattggcc tgactcacca gcagtttttc accgccgttc
    13141 aatttcagtt caaaacgagt tgccgcgccc tgatattgca ccgcctggat cgtgccattg
    13201 gcctgcagtt caccaggggt gttgaggcgg atatgttccg gccgcagggc gaagcttccc
    13261 gtcatgccac aaagtttctc tgccatcagt ccatcaaaaa cattcgatgt accaacgaac
    13321 ccggcaacaa acggcgtgcg cgggcgcata tagagatcgc gcggggaatc gacctgctca
    13381 atgcgtccat tattgaaaac cgccacacga tcggacatcg ataacgcttc gccctgatcg
    13441 tgggtaacgc agataaaagt gataccgaga gactgttgca gttttttcag ctcaagctgc
    13501 atctgctcac gcaatttgag atccagtgcg ccgagcggtt catccaacag caatacgcgc
    13561 ggttcattca ccaatgctct ggcgatagca acccgctggc gctgaccacc agaaagttgt
    13621 gacggtttac gttgctgtac aaaccccaac gccactttct ccagcgcctc ttgcgccatt
    13681 gcgtgccgct gctttttatt cacgcctttg accatcagcc cataggcgac attgtcaaga
    13741 atcgacatat gcggaaatag cgcgtagtcc tgaaagacag tattcacgtc ccgctcccac
    13801 ggtggcagat tgctggcggg tttaccaaag atagagatag ccccgccgga aagctgttcg
    13861 aagccagcaa tcaggcgcag gcaggtggtt ttgccggagc cggacggccc cagcatagag
    13921 aagaactcac catcttttat cgcaatactg acgccatcta ctgcgcgcac gtcaccgtac
    13981 aaccgcgaga cgttgtcaaa ctccactgcg tacgtcataa agcaccccag cgaagttagc
    14041 gaccgcccat aatggcaatg taatcctgcg tccagcgact gtagggaaca aacttgcccc
    14101 cttctgctat aggcgttttc cagaaggcta ttttatcgaa atagttaaaa ccgtttgttt
    14161 cacaaccttt ctcgcctaat aacgaactgg ctttacaccc ttccggcacg actggtaacg
    14221 agccaaacca ggccgccaca tcgccctgca cttttggcgt taatgaccag ttcatccatt
    14281 tgtaggcgca aaccggatgt ttcgcttcgc tatgtagcat ggtggtgtca gcccaaccgg
    14341 taacgccctc cttcgggaaa acggtagcga caggctgacc ttcggctttc agggcgttgg
    14401 tctgataggg ccaggcactg gaagcgacca caccctcgtt tttgaaatcg ctcatttgca
    14461 cggtagtgtc atgccagtag cgatggatca aactatgttg atcacgcagc actttcagca
    14521 ccgcctggta ctgttcttcg gtgagttgat acggatcgct gatgcccaac tgcggctgag
    14581 tggctttaac gaacaacgca gcgtccgcaa tgtagatagg gccatcataa gcctgaacgc
    14641 ggcctttatt gctcttgccg tcctgcagat tttgctcaac aaaaaccact tgccagctat
    14701 ccggcggcgt cgggaaggtt ttagtgttgt acatcagcag gttcggcccc cattggtaag
    14761 gtgtgccgta aactttgccg ccaacgttaa accagtcgcc tttaaccacg cgcggatcaa
    14821 gcgttttcca gttgggaatc aaggcggtat taatcggctg cacgcgttta cccataatca
    14881 aacgcagcga ggcatcgccg gatgccgtaa ccagatcgta accccctttg gtcatcagac
    14941 tgaccatctc atcggaagtc gcggcggttt taacattcac cgcgcagcct gtctcttttt
    15001 cgaattgcgt tacccagtcg tattgtttat cagtttgtcc gcgttcgata tatcccggcc
    15061 aggcgataat atccagtcgc ccttccggtt tatctaaatt ggtaggcggt tcggcggcgt
    15121 gagcggtcat tattgtcatg ctgagcgcac acaggctgct gcgggcaaat gtcttgctca
    15181 taaggtctta ctcctgtcgt aattaattgt tcagccgccg tgccgtaaaa atatctcctt
    15241 gtttaaccat agacggcggc ttgcaggcac accttcgccc ctgagaaaat ttaatcaggt
    15301 tgtgagctac cgcgcattat gccgtctgta aggttatctt ctggagtata gacaaggagt
    15361 taggaaatat gactgctatt cgaaattcat attacccaat ctaagaggaa ttaaattaaa
    15421 cgctattaca gccgttcttg aataagtttg cctaattgtt ttaccgcctg ttcttcacgt
    15481 tctccccaca gccacgcggt attaaaacga aaaaaacgtg accagttttc accggtagaa
    15541 aacattttac ctggcgcaat actgatatga tgcgtcagtg ccgccaggct caattcgccg
    15601 gcatctaacg gctcggggag ctccagccag agaaaataac cgctgtcatt atgatgaatt
    15661 ttcacttccg caggcagata acgcagcagt gcctgccagg cacgttgttt acgttccgca
    15721 agctggcgac gcaggcgacg aagatgggcg tcgtatcggc gcgtggaaag gtaatccacc
    15781 agcgcaagct gcatcggtga gctggtggaa agcgtactca tcagctgcaa gcgttgaatt
    15841 ttacgtgcat gttttccggc ggcgacccaa ccaatacgaa aaccaggcac cagacatttc
    15901 gaaaacgaag agcaatgcaa aacgccatcg tggcgatccc acgctttcgc aggcagcggt
    15961 ttttcccgtc caaaataaag ttcgctgtaa acgtcatctt caatcagcgt tacgttgtac
    16021 tgattgagca acgccaccag ttgtgctttt ttttgcggcg ttaaggtaaa tccgagtgga
    16081 ttctggctat tagtcatcag ccagcacgct ttcaccggat actcctgcaa cgccagttcc
    16141 agcgcctgaa gatcgatccc ttctttaaca tccgtcgcca ccgataacgc cttcagccgt
    16201 agccgctcca gcgcctgcaa cgcaccgtag aaacaaggat tctctactat cacccaatcg
    16261 cccggttcag ttaccgcttg caaactgagg tttaatgcct ctaacgcccc ggcagtgatg
    16321 acaatttcat caggagaaat ggtgatcccc tgtaaggcat agcgacgggc aatagcctga
    16381 cgcagttctg cgtttcctgg cggcaagttt tcaatcacgc tcatcgccgt ggcggttttg
    16441 cttacctgcg ccagcgagcg gtttagttgt tggaggggga aaagtcgcgg gtcgggaaag
    16501 gccgaggcaa acggaacgac cgacggatcg cgactggcct gcaacatatc aaaaatataa
    16561 gtgttgatat cgactgcttc atctcgagtg actggaatga ctggcacttt cggcattttt
    16621 attgcctgtg gcgcaacgta ataacccgac tgcggtcgtg cgataatata tccctgactt
    16681 tcgagcaact gataggcatg gctgacagtc ataaagctca tgccagaaag cgccacctgg
    16741 tcacgcaacg aaggtaaacg atcgccgggt tgccagatac ccgacgcaat ctgctcgcgt
    16801 aattgttctg caagctgctg gtattttttc attatcgaat cgtaattatg tgcagatgat
    16861 tcggcagtct atatcagttg ttaaaaatga caacttttcg ttaactgtaa ttaaaccatc
    16921 accagcgata actctttgcc aagcgccttt gccgcgagct ggacggcgtc gatttttgtc
    16981 gcatgatgca agttaaatag gcgagtaatc tcttgtttag gtttgccaat tcgcctggct
    17041 aactcttgct gagtgatttc tgactgtaaa aaagcattta acagcaatac cttagaggcg
    17101 acgctcaaag gtacttcaat aaagtgatca tgactattta atggcgaagg taaagggata
    17161 agctcgttat cttcaaaata aaaatcaaat gcggtcagta aagcatcttt tgccgcttcc
    17221 atcgcttcag cgacagtttc gccctgcgtc aacgcttcag ggatatccac aaaagaaacc
    17281 atataaccgc cttccggcgc gggtgtaaga gtgacgggat aacgcataat ttcgactgtc
    17341 tctcgcatgt cattaacctt tttaattggc gattaactca aaccgagttg tttcaggatt
    17401 gctttacgca atggttcttt aatctcatcg caggggtgac gcggcatgac actgcgcctc
    17461 ccatgaaacc tgagtttcaa atggttgctg ccattcgcta catcgacgcc ctgagattcg
    17521 agccaacgtc tgaactcgct ttgtttcaca tcgcctccct gcggctgagt atatcaacaa
    17581 agtaaacatt agtgtttacc tccgtgtgta atttgctcga aaatagcact aaatcgccgc
    17641 aacggcagaa tgcaatgcaa aatcaggaag gaagattcca gaatttcttt gcgctaattt
    17701 gcaaaactga ataataacct caagaaacga tcggcatcat agcggcaaaa ggtaagaaaa
    17761 aggtggcagg atgtttacga aggcgttatc ggttgtctta ttaacgtgtg cgctgttctc
    17821 aggacaactc atggcagggc acaaaggaca tgaatttgtg tgggtaaaga atgtggatca
    17881 tcagctgcgt catgaagcgg acagcgatga attgcgtgct gtggcggaag agtcggcgga
    17941 aggtttgcgc gagcattttt actggcaaaa atcgcgcaaa ccagaagcgg gacaacgttg
    18001 agtcgtttga tgccggatgc ggcgtaacgc cttacccggc aggcaatatc atgcagattc
    18061 aataagttgt gagtaaccag aaaactggcg ttttaccctt ttcgcttcgg caatgttttc
    18121 agcagctcat ccggacttac ggatgccact acgcttcccg gtaacggcat tttcagtatg
    18181 tgatttttga ttttgccaat gacgtgcatc tcgcatgggc ggcaatcaaa ctttagcgtt
    18241 aatacttcat cgccgttaac cagttgcatt ggcgtcgcct tccagctttt gatattgcct
    18301 ttcgcctgtt tcgggcacag attaaaggca aagcgcagac aatgcttggt gatcatcacc
    18361 gggacttcgc ccttctcttc atgtgcctca tacgccgcgt caatcagctg cacaccatag
    18421 cgatgataaa attcacgcgc tttctggttg taaacgttcg cgaggaaact cagatgtgtt
    18481 tgcggataaa ccggcgcagg atcagcaacc ggtttacggc tgccgcgctg gtaactggca
    18541 agacgcgcag catccagcat gtcggcagct tcacggcgga actggtttaa cagactgttt
    18601 ggtacaaaca gcgcccccgg caaattaatt tgcacatcgc gggcgtaata gagggtttgc
    18661 cccagttttg ccagaccatc cttcagattg ttcattgctt tttctgcgtt attggcttcg
    18721 tcgaactgcc catccagcgt atgcgttatg ctgaccccct cttcactggt gagagtcaga
    18781 atcaattgtt cctgccaacc gcccagttca atgtctaccg ccacccgacg ttcgctagag
    18841 gtttttgtca gtgcctgctg ccagttatga tcaagattac ggtttagtgg gtgatgcggg
    18901 cgaattttgt gcaaatctgc tggcatttca ttgggccaga cgcggtactg attttctccg
    18961 gttttctcga ccgtattggc acgaaaaccg acgacttcac gtttaatcaa cacgttcagg
    19021 ccatcgccat ttgccagtgg ctcggtaacg gcaacatcga gatgatcttt cgccactttc
    19081 actacttcac ctaccggcag gccgataaat ttcggcgaat cgaacgcgcc aatatcgcct
    19141 ttacgggcat tcacaaaata atctgtgcta ccacggtgga aagtcttttc cgtcgatgga
    19201 acaaagaaat gttcagtacg acctgatgaa gcgcgcgcca gatcgccacg ttcttcaata
    19261 atggcatcaa gcatctggcg ataatgggcg gtgatattct tcacgtagct catatctttg
    19321 taacgccctt caatcttgaa ggaacgtacg ccagcatcaa tcagcgcgcc gaggttggca
    19381 gtctgatcgt tatctttcat cgacagcaga tgtttttcat aggaaaccac ccgcccctga
    19441 tcgtctttca atgtgtatgg caaacggcac gcttgcgagc aatcgccacg gttggcgcta
    19501 cgccctgttt gcgcatgaga aatgtagcac tgacccgaat aggccacgca cagtgcgcca
    19561 tgaataaaga attcaatggt cgcgtccgtg gcctggtgaa tcgcgcggat ctgatcaaga
    19621 ttcagctctc gcgccagcac aatctgcgtg aagccaacat cagagaggaa cttcgctttt
    19681 tctactgtac gaatgtcgca ctgcgtactg gcgtgcagtt caatcggcgg aatatcaagt
    19741 tccagaatcc ccatatcctg aacaatcagc gcatcgacac cggtctggta gaggtcagta
    19801 atcagccgtt gcgcgggttc cagctcatca tcatgcaaaa tggtgttaag cgtgacgaaa
    19861 atttttgcac cataacgatg ggcaaacggc accagctcgg caatatcttt caggctatta
    19921 ctggcattat gacgggcacc aaaaccaggg ccgccgatat aaacagcatc ggcaccgtgc
    19981 aaaatagctt cgcgggcaat ggcggcatcg cgtgccgggc ttaacagttc aagtcgatga
    20041 gaagatacgg tcacattctt atccagtagc tgaaaaatgg cggctatttt agtcacaagc
    20101 gcgacgttgc gaaatagttt tcccgcttaa ctgcgcgggt aatggatgag ggaatgaaaa
    20161 tgtacggttt gctccccacc attacgatag atatgcgtga cgtctgcagc aaatcgaacg
    20221 ccttccccac aattaagggt ctgccattca ccatcaacgc acagatcaag ttgtccatca
    20281 atgacgacga catgttcgat tacccccttc tcatggggcg tcgattcgct tattgcaccg
    20341 ggtgccatct gaatcgagaa gtgttcaaag cagagttgtg gatcataagg aaacagcgaa
    20401 atgatcacca tcgcctgctg ttgtggatca taaactgaag gcgtagcgga ctgtggcgga
    20461 gaaataaatg tcgaaaatgg cacgttcaac ccagtggcaa ttttccataa tgtcgcgacc
    20521 gtcgggctgg actcattacg ctcaatctgt cctaacattg ctttagaaac gcccgtcgct
    20581 tctgccagtc gcgaaagact ccagccgcgc tgctgacgta gttgtttaag tgtggtggat
    20641 aaaaagcgag cgagattttc catgcgactc ctccggcaaa acggaagttt atcacttgtg
    20701 cgttataacg gacaaatgct acggtgcctg tacgctataa cgcacgaggt gactatgcgt
    20761 ctgttttctg ttcctccacc cacgctactg gcggggtttc tggcggtatt aattggctac
    20821 gccagttcag cggcaataat ctggcaagca gcgattgtcg ccggagccac cactgcacaa
    20881 atctctggct ggatgacggc gctggggctg gcaatgggcg tcagtacgct gactctgaca
    20941 ttatggtatc gcgtacctgt tctcaccgca tggtcaacgc ctggcgcggc tttgttggtc
    21001 accggattgc agggactaac acttaacgaa gccatcggcg tttttattgt caccaacgcg
    21061 ttaatagtcc tctgcggcat aacgggactc tttgctcgtc tgatgcgcat tattccgcac
    21121 tcgcttgcgg cagcaatgct tgccgggatt ttattacgct ttggtttaca ggcgtttgcc
    21181 agtctggacg gtcaatttac gttgtgtgga agtatgttgc tggtatggct ggcaaccaag
    21241 gccgttgcgc cgcgctatgc ggtaattgcc gcgatgatta ttgggatcgt gatcgttatc
    21301 gcgcaaggtg acgttgtcac aactgatgtt gtctttaaac ccgttctccc cacttatatt
    21361 acccctgatt tttcgtttgc tcacagcctg agcgttgcac tccccctttt tctggtgacg
    21421 atggcatcgc aaaacgcacc gggtatcgca gcaatgaaag cagccgggta ttcggctcct
    21481 gtttcgccgt tgattgtgtt tactggattg ctggcactgg ttttttcccc tttcggcgtt
    21541 tattccgtcg gtattgcggc aatcaccgcg gctatttgcc aaagcccgga agcgcatccg
    21601 gataaagatc aacgttggct ggcagctgcc gttgcaggca ttttctattt gctcgcaggt
    21661 ctgtttggta gtgccattac cgggatgatg gctgccctgc ccgtaagttg gatccagatg
    21721 ctggcaggtc tggcgctgtt gagtaccatt ggcggcagtt tgtatcaggc gctgcataat
    21781 gagcgtgagc gcgacgcggc ggtggtggca tttctggtaa cggcaagtgg attgacgctg
    21841 gtcgggattg gttctgcgtt ttggggatta attgccggag gcgtttgtta cgtggtgttg
    21901 aatttaatcg ctgacagaaa ccgataataa aaagcgccat ctaacgatgg cgcgtctgga
    21961 actacttttt gttaacgtca aacatggtgg catcggtcgc catgtcatca ataacttgtt
    22021 taatattttc gaaagccatt ggcgtacttt cgttattcag gtctttacct tcgccctgac
    22081 gaacgacttt gataaccggt ttattagtcg ctgcatcaat cagctcacct tcaaaataca
    22141 agcgagtatc catggtacgg tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa
    22201 caggaaccac ttcatagaat tgcagccctt ctttgctggt atctacaccg gtaatggccc
    22261 cacggaaaat cagactacgc ggccccgcgg tggtaaccag tggtttacgc tgcgctatcg
    22321 cttctttcat ttcggtgttg gtatagttca aaattttatc cagaactttc tgccctacct
    22381 gggtcgaagg tttcggtacc ggataataag tgattgggtt ccagacgatg ctgtcatatt
    22441 tgctttgatc aaaactcggg tctacccaac gtaaaaccgg tttacctgta gccgaggttg
    22501 tttcttttaa atcagagtaa ttgtttaaaa aaccagaata tttatctggc tgggtgattt
    22561 tagaggcaca accagacaga gccaacaagc cgcataaagc tgcaactttc gcaaatgatg
    22621 tggtacgcat gaatagtttc ctgagatgat tatattactt tttaagttat agcaaaaagc
    22681 gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata aattttttta gccccttttc
    22741 agacgacata gtcaatgccg caaaaggggc taagtcaatc atcagtagtt aaatttcagt
    22801 aagttaagct gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa
    22861 atatgcttac cgaaaagaag gacagtaaac catctcagcg tggggaattt tcacgcgctt
    22921 tgttcttacc gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat
    22981 taccgttggc gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accatctccc
    23041 agccttcgta atatcgacgt aattctccct ctttgaaggc aaacgggaag ccgacggtac
    23101 atggataatc agcggtatcc atcgccgcca caatcaggtt gtaaccgcca ggtttagtgc
    23161 aacgttgcat attggcaatc agccctggga tggttttagc ctcaaggaac atcagcacca
    23221 cagtcgaaag aataaaatcg tactctccat caaatgtgag gttattcaga tcaacgaccc
    23281 gggtgtgtaa attatccaga ttttcaatgg atttaatgcg ctcgacgttg gcgatactca
    23341 tggcattttt atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac
    23401 gaccattgcc acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca
    23461 gtacttcaga gtgtgtgcgg gttaattcat atttatcagt aaaataattt tcgtcacgaa
    23521 tgatcattct ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt
    23581 tccctgcatc agaagcgcca aagtacggat gagcagtatt gcaataataa aattggtaaa
    23641 gataaacagc ggcaccgcca gcgtatggaa aaatccatta tcgctgccac tccccagatg
    23701 caaaccggtg gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa
    23761 tggctgggag agataccatg gcatcagacg tagcataaac agcagttgca gcagtccata
    23821 accgaaaagc attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca gccaggcact
    23881 acaggccacc agcgcaggag cgagctgaat gccgagtgat gtccgcagtg ccgtgggtaa
    23941 ttctcccgaa ctacgcagac gctgcaagat caccggttca aggcttagcc atgagaaaac
    24001 gcctgcgcct aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc
    24061 gctgataaag ttgttggcaa ctgtcggcag atacagtcca ggcgtagtag cttcttcagg
    24121 gtgagatccg cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac
    24181 accaaaactg aacaggcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac
    24241 cagcatcgtc gttgcaggaa acaaactcac aaaactgctc agcactggat ggcgaacttc
    24301 cgccagcacg ctatgcggaa agcgtatgag tcgggtaata aatgcgctag tcaataatcc
    24361 ccagatgatc atcgccagaa tcaccagccc atcccctaac cagtggctga cctgccaaac
    24421 ctggctggca tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta
    24481 gcctgccggc aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt
    24541 ataaagtata tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa
    24601 tttgctacgg ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata tcgtctaagt
    24661 gaagaacagc gagcctttag ttatcaggaa gatggcacta aaaaaaatgt gttattacgg
    24721 cagattatcg ccattagcga ttttaacgat gtgatagcag gaaccgctgg cggctggatc
    24781 gatcgcgaaa cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt
    24841 gcgttcggcg gaacggtgat ttccggtaat acgcgcatca ccggcaccag cgtactgtgg
    24901 ggagaggttt acgcaacgga taatgtctgg atcgataata gtgaaatcaa ccagggtgca
    24961 tatataagtg acagtgtcac cattcatgac tcactggttt gcgggcaatg tagaatattt
    25021 ggtcacgcct taattgatca acattctatg atcgtcgccg cacaaggctt aacgcccgac
    25081 catcaactcc tgctccaaat ttatgatcgc gcaagggtta gcgcctcgcg tattgtccac
    25141 caggcacaaa tttatggcga tgccgtcatc cggtatgctt ttattgaaca tcgcgccgaa
    25201 gtttttgatt ttgccagtat tgaaggtaat gaagagaaca acgtctggct atgcgattgc
    25261 gctaaagtct acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccaacg
    25321 attcattaca gttcgcaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa
    25381 catcatgtct tgattggcgg aaatgccgtg gtacgtggcg gaccgattct gctcgacgag
    25441 cacgttgtaa tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg
    25501 gagttaaccg accacgccgt cgttgaagcc tttgatggcg acacggttca tgtccgtggt
    25561 ccaaaagtca ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg gttattatga
    25621 atcgataata cgcgcgtata agttcacatc atcataggca tcattcagga actcagcctg
    25681 tttcaggcaa ccttcaagga taaaaccatt gcgcaaagcg acctggttgc tttgcggatt
    25741 gtccacccga catttgatca cgaagcgtct aagttcacca gactgagcgt aatgatgaat
    25801 cagtgcctgc agcgcctgag aaatgatccc ctgcccctga tgagattcgt ccagtcagta
    25861 gccgatttca gcggttttat tcagtggttc aatacgatta aacgagataa cgccgataag
    25921 ttcatcttct ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt
    25981 accctgcacc gtttttcgcg tgtcctcttc aatctgaacg aactgcggcc agtttagcga
    26041 ctgctgtaac caggttttat ttttacagat taactgataa agaggagtga cgtgattttc
    26101 tgcaacagca tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc
    26161 agtcaatcca tgcagttgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc
    26221 tctttgacgc tctcatgctg caggattttg agcatctcat cttttaactg tagcttttgt
    26281 tttttcatgc ggaccacttc cgcattgtac cctcgatcgt cggaaccttc ctttctggca
    26341 atttcatgat caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt
    26401 ttcagacggg atattaaatc tcgatattct ggaaacatac gcactccctg ttagtggctg
    26461 acaaggtgta tctgaaatac acaccatcag attactcatt taacttaggg aaaaatgcga
    26521 gcgggatcgt gtttaaccag acggcacctc ccgaagaagg tgccgaagaa aaaacgatta
    26581 actcatcacg cggtcgtcaa catactgacg tttatccggc gctggcggga aatactgata
    26641 cagccatgtt tcactgatag cgtcgccctg acaacgcaga tacatccgca tatcgaccgg
    26701 atcagtggag tccgaagtcg gataccagtc aaactgaata cgataaccat caatgggttc
    26761 aatatagaga atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat
    26821 gccttttggt gcggcagctt tcagatcacc accaacgaaa tcgacggcaa aacgacgcgc
    26881 ccatttttcg ggatagtgtt cacctggtgc ccaaccttcc gggaaaccgc ccatgccggt
    26941 acgcgtcgcc ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag
    27001 acgatactgg aatgcaaact catcacccgc ctttacagct ttttctggct gccagaagca
    27061 gacaatgtta tccagcgttt cgcccgttgt tgggatttcc atcaggccga tggtgccctt
    27121 accccacttg ttacgcggtt ccacccacag acttgggcgt ttgttatacc agcccataat
    27181 gtcctgataa tgggagaaat cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt
    27241 gtcggtgtaa gcattgaact gcaatttttg cggattattc agcggacggc aaatccactc
    27301 gccgttgccc cgccacatgg acagacgatc agagtcatga atttgcggat gaatggtgtc
    27361 gcacatccga cgttcattag taccgcagct gaacatactg gtcatcggcg caatgcccag
    27421 ctgtttaatg tctttgcgcg catacaggtg attttccaca tccataatca cctgactttt
    27481 ctcacaatgg atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata
    27541 aacggtaaag gtagttgccc ccggttttac cgtatcaaac cagaaggcgg taaagtcggg
    27601 gaactcttct ttactgtcgg tgtaagtgtc aatcgccagg ccgcgggccg acaaaccgta
    27661 ttgatatgtg tcatcaacgg cgcggaaata actcgctccg aggaatgata ctacatcacg
    27721 gcgcgccagt tcgggggctt taaacacgcg aaaaccggca aagccgagat cgctttgccc
    27781 ttctaattgt ttggtatcaa cacctgcatc gttgtatttg aacaactccg ggcgaaagtg
    27841 aatttcacgc gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa
    27901 tcccattccc atatggaaga actgagcgtc cagttgacgg ttctcaacgt tatgccagag
    27961 cgatttttcg gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagcgt
    28021 gtcaggtaac ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt gcgccattga
    28081 ctgtagaatg gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatctgccgc
    28141 gaatgccgcc tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc
    28201 cattgaacct ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg
    28261 tgaatggtcc cgcgctggtc gttttcagcg gcaagaaagt gcttatttat gaccgctcac
    28321 tctagacaat atttattaat aatccaattg ttgaactgag agaatatgca taaatcggca
    28381 atttttttat gtcgatggga tgagcctgat tcgtttgtaa aatcaggcaa atttttgcaa
    28441 aggcggatgg tcagaattta aagcaaacca cagcgacgtt tactgcgttc ttccgcttcc
    28501 tgataaagct ggaactcatc caaaaccgga caacccaacg cctgctcaaa tgcttcgcga
    28561 ctggcattac cgtggctgcg tgcctgcgtt tcattaccca gatttgactg gaagatcccc
    28621 gccgcgctaa cgggcaagaa atcttcatag gtaatgggtt gcgccgcgac ccaaccacgt
    28681 tcaattaagg gctgtggatc gtctccggga tgaatcgcct gacgatgcgc ctcacctgaa
    28741 ggcgtcagac ggtaccggaa ccatgccagc ccttgctgac gcattaaaaa ctcactgtca
    28801 ggaaaagtgc ggaaggtttc ctgtaaatgc atttggtgag tgagattatc ctgcccggtt
    28861 ccagcgttac gcagaagatc atcatacagt tgtcgacctt tcggcgttaa tgccacgcca
    28921 cgctgctcaa tttcaccaaa gcgcgcagta tgcgtgccct gtttctgccc cgcaaacaac
    28981 accgtctctt ccagtgcttt aaagctggtc tggcgtagta aaatcggtac ctcgcggcgc
    29041 ggcggccctt cgatcagaat ttttggttca attccgcatt caggcatcat cgactgcacc
    29101 cggtcaatat ccagcgtacg tggcgtcaga tggttgatat ggcatccagg aaaacagacc
    29161 acatcagcaa tcaaccgatg ttcgttgtgc aaggcgcgat aggtttcttc atctaccgtt
    29221 gctgactggt gccaacggaa cgtttccagg gcctcctgca caaactcctg tgcctgtgtt
    29281 tcgttaaaac cgccccgctg gtcatactcc tctaacagtt gtcgacaacg tggggtgaag
    29341 atatcgcgct gacgcagaat ctccgccgct ttctggcgca aaatttcgtt ctcgataagc
    29401 tcaaggcgga gtaaggaggt aaaaacgcgg aagggattac gcgccagaga agcatcatca
    29461 atgggccgaa atgctgtcga atgtaccggc acccctgcct gcgagagatc gtaatagctc
    29521 accgggtaca tccccataat ggcaaacatc cgccgaagag tagcaagctc ttgtgcagtc
    29581 ccaacgcgaa tcgccccatg acgttcaaca ttcagtcgcg ccagctcgtc tgcatttacc
    29641 attttttcgt gcagttgagg attgttttcc agcacagcca gattcacatc agctaccagt
    29701 tccagcagcg tgccatattg cggaacttct tgctggtaca tggctgacat tgcctgcgaa
    29761 aactgttccc gaatctcatc cgccgtgatg ctgttcgcca tagtgtctgc ctccagtggt
    29821 cagtaatctg gagtgtaggt aaccgcattc actctggcgg gaagaattta caaactgtga
    29881 tctcgccgcg aaaacatcaa tattatccat tttgct
//
LOCUS       DAZGXS010000049        29738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGXS010000049 DAZGXS010000000
VERSION     DAZGXS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(96..1145)
                     /locus_tag="ACHKG5_002277"
     CDS             complement(96..1145)
                     /locus_tag="ACHKG5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0868379.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1277..2494
                     /locus_tag="ACHKG5_002278"
     CDS             1277..2494
                     /locus_tag="ACHKG5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0868380.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2498..3055)
                     /gene="dcrB"
                     /locus_tag="ACHKG5_002279"
     CDS             complement(2498..3055)
                     /gene="dcrB"
                     /locus_tag="ACHKG5_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0868381.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(3128..3793)
                     /locus_tag="ACHKG5_002280"
     CDS             complement(3128..3793)
                     /locus_tag="ACHKG5_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0868382.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            4014..4259
                     /gene="tusA"
                     /locus_tag="ACHKG5_002281"
     CDS             4014..4259
                     /gene="tusA"
                     /locus_tag="ACHKG5_002281"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0868383.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4361..6559)
                     /gene="zntA"
                     /locus_tag="ACHKG5_002282"
     CDS             complement(4361..6559)
                     /gene="zntA"
                     /locus_tag="ACHKG5_002282"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0868384.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(6633..7259)
                     /locus_tag="ACHKG5_002283"
     CDS             complement(6633..7259)
                     /locus_tag="ACHKG5_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0868385.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            7400..7759
                     /locus_tag="ACHKG5_002284"
     CDS             7400..7759
                     /locus_tag="ACHKG5_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0868386.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYQALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7762..8031)
                     /locus_tag="ACHKG5_002285"
     CDS             complement(7762..8031)
                     /locus_tag="ACHKG5_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0868387.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8021..8617)
                     /gene="rsmD"
                     /locus_tag="ACHKG5_002286"
     CDS             complement(8021..8617)
                     /gene="rsmD"
                     /locus_tag="ACHKG5_002286"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0868388.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            8767..10263
                     /gene="ftsY"
                     /locus_tag="ACHKG5_002287"
     CDS             8767..10263
                     /gene="ftsY"
                     /locus_tag="ACHKG5_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0868389.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10266..10934
                     /gene="ftsE"
                     /locus_tag="ACHKG5_002288"
     CDS             10266..10934
                     /gene="ftsE"
                     /locus_tag="ACHKG5_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0868390.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            10927..11985
                     /gene="ftsX"
                     /locus_tag="ACHKG5_002289"
     CDS             10927..11985
                     /gene="ftsX"
                     /locus_tag="ACHKG5_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0868391.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12230..13084
                     /gene="rpoH"
                     /locus_tag="ACHKG5_002290"
     CDS             12230..13084
                     /gene="rpoH"
                     /locus_tag="ACHKG5_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0868392.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13355..14458
                     /gene="livJ"
                     /locus_tag="ACHKG5_002291"
     CDS             13355..14458
                     /gene="livJ"
                     /locus_tag="ACHKG5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0868393.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            14608..14874
                     /gene="ataR"
                     /locus_tag="ACHKG5_002292"
     CDS             14608..14874
                     /gene="ataR"
                     /locus_tag="ACHKG5_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP0868394.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            14881..15408
                     /gene="ataT"
                     /locus_tag="ACHKG5_002293"
     CDS             14881..15408
                     /gene="ataT"
                     /locus_tag="ACHKG5_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP0868395.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(15405..15788)
                     /gene="panM"
                     /locus_tag="ACHKG5_002294"
     CDS             complement(15405..15788)
                     /gene="panM"
                     /locus_tag="ACHKG5_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0868396.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            16212..17321
                     /gene="livK"
                     /locus_tag="ACHKG5_002295"
     CDS             16212..17321
                     /gene="livK"
                     /locus_tag="ACHKG5_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0868397.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            17369..18295
                     /gene="livH"
                     /locus_tag="ACHKG5_002296"
     CDS             17369..18295
                     /gene="livH"
                     /locus_tag="ACHKG5_002296"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0868398.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            18292..19569
                     /gene="livM"
                     /locus_tag="ACHKG5_002297"
     CDS             18292..19569
                     /gene="livM"
                     /locus_tag="ACHKG5_002297"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0868399.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            19566..20333
                     /gene="livG"
                     /locus_tag="ACHKG5_002298"
     CDS             19566..20333
                     /gene="livG"
                     /locus_tag="ACHKG5_002298"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0868400.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            20335..21048
                     /gene="livF"
                     /locus_tag="ACHKG5_002299"
     CDS             20335..21048
                     /gene="livF"
                     /locus_tag="ACHKG5_002299"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0868401.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            21167..21913
                     /locus_tag="ACHKG5_002300"
     CDS             21167..21913
                     /locus_tag="ACHKG5_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0868402.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            22273..23589
                     /gene="ugpB"
                     /locus_tag="ACHKG5_002301"
     CDS             22273..23589
                     /gene="ugpB"
                     /locus_tag="ACHKG5_002301"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0868403.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            23687..24574
                     /gene="ugpA"
                     /locus_tag="ACHKG5_002302"
     CDS             23687..24574
                     /gene="ugpA"
                     /locus_tag="ACHKG5_002302"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0868404.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            24571..25416
                     /gene="ugpE"
                     /locus_tag="ACHKG5_002303"
     CDS             24571..25416
                     /gene="ugpE"
                     /locus_tag="ACHKG5_002303"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0868405.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGINSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            25418..26488
                     /gene="ugpC"
                     /locus_tag="ACHKG5_002304"
     CDS             25418..26488
                     /gene="ugpC"
                     /locus_tag="ACHKG5_002304"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0868406.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            26485..27228
                     /gene="ugpQ"
                     /locus_tag="ACHKG5_002305"
     CDS             26485..27228
                     /gene="ugpQ"
                     /locus_tag="ACHKG5_002305"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0868407.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(27215..27655)
                     /locus_tag="ACHKG5_002306"
     CDS             complement(27215..27655)
                     /locus_tag="ACHKG5_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0868408.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            27775..29520
                     /gene="ggt"
                     /locus_tag="ACHKG5_002307"
     CDS             27775..29520
                     /gene="ggt"
                     /locus_tag="ACHKG5_002307"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0868409.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         6982 a         7573 c         8241 g         6942 t
ORIGIN      
        1 tttaaattaa ttttctgaat aatgtttcat catttcctca aatcaaatgg ccactgttag
       61 ataaacggtg gccattgcct gctcaggagg tgctattaac ccggtaaccg acttttcggt
      121 ctgcccggcc ccagtaaaat cgccagtttg ctgccgcctt tcgtcgtttc catccagatt
      181 ttacacacgc tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc
      241 caaattaaca atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc
      301 cgtggttcta aaatattgcc gatgaccata tggaccacta aaaacaatgc gccgaccaga
      361 atacattcgt aaacaccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt
      421 accgcgccga tattgggcac gtagttaagc aaaaacgcca gtactgccca catcagcgca
      481 aactgtaccc ccatcagctc cagccccagc cagacgatga cacccgtcca taaactgagt
      541 agcgtcttta acgccagata atgcgaaacg cctttaagtg cgcggtgtaa tcccgcgatg
      601 tgaatctgtg ggttattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac
      661 agcataaaaa ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg
      721 gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc
      781 tccggcgaca tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc
      841 gtcagctcct tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt
      901 acgccgacca gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg
      961 gggcgttgta ctccccgacg aataaaccag gtgacgagcg ggttgaggac gatggcaaaa
     1021 aacagcgcca gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc
     1081 actaccagcg aggccagctt gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt
     1141 tccattgggg cttccttgtg actttttgta ttaagtgtag cgggagtcac gacggcaata
     1201 ttctgattct tactgctgat tttcacgtca gcacgtggta aaaatgaaac actgttgtaa
     1261 aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct
     1321 gaatttgcgc attctctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt
     1381 gccgctcgct gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ctttctgggc
     1441 aggattggtt atcagcctgc aatatttcgc caccttgctg agccgtcctc atgccggacg
     1501 ttacgccgat ttgctgggcc ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt
     1561 gagcggtctg ggatatctga cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt
     1621 attactttgc ctgggacgcg tcatccttgg gattgggcaa agttttgccg gaacgggatc
     1681 gaccctgtgg ggtgttggcg tggttggctc gctgcatatc gggcgggtga tttcgtggaa
     1741 cggcattgtc acttacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca
     1801 ctggggcggc ttgcaggcgt tagcgttaat tattatgggc gtggcgctgg tggccatttt
     1861 gttggcgatc ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc
     1921 ggtgcttggg cgcgtctggc tgtacggtat ggcgctggca ctggcttccg ccggatttgg
     1981 cgtcatcgcc acctttatca cgctgtttta tgacgttaaa ggttgggacg gtgcggcttt
     2041 cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat
     2101 taaccgtatc ggcggcttaa acgtagcgat gatttgcttt agcgttgaga taatcggcct
     2161 gctactggtt ggcgtggcga ctatgccgtg gatggcgaaa atcggcgtct tactggcggg
     2221 ggccgggttt tcgctggtgt tcccggcatt gggtgtagtg gcggtaaaag cggttccgca
     2281 gcaaaatcag ggggcggcgc tggcaaccta caccgtattt atggatttat cgcttggcgt
     2341 gaccggacca ctggctgggc tggtgatgag ttgggcgggc gtaccggtga tttatctggc
     2401 ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc
     2461 ggaacacgtc cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa
     2521 tgatgttttc tgcggtggtc tgcgcttttt gctgatcgtc agctggcaga gtaatttgca
     2581 tggtcagcag ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt
     2641 tcgcggagat aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttat
     2701 tggtgaccac ttgcagctgt ggatcgcggc tacgttgctg atcttccaga cgcttcgcca
     2761 gcaccgccag atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg
     2821 tggcgtcgga ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg
     2881 tcatatccgc tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg
     2941 tagcgttact ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg
     3001 ccgcaagccc cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact
     3061 tcttcctttc gataaacggc cataacggct cattcatcca tcttatcaca actctgataa
     3121 cgaaccttta actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca
     3181 gcatattgag taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga
     3241 aacagtaatc gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac
     3301 gccagaaggc aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac agtgtggacg
     3361 ctgtcggtgc cagccaccag cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga
     3421 ggatctgtcc cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt
     3481 tgaagtgggc gagtgcgccg aatccctgcc atgaacccat atagaatagc gacgagatga
     3541 cgtaggagat cagcaatgcc gggatcatca ccgcaaagat gatacgacgg gccagcggtg
     3601 cgccaaaaat acgtacggtc aggtcggtag cgagaaagat aaacggaaag ctaaacgcgc
     3661 cccaggtggt atggaaacca aaaatggaga cgggaagctg aaccagatag ttactggagg
     3721 tgatcaccag cagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg
     3781 agaaaacgtt catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta
     3841 cgacacgtct gttaccttaa cataataacg tgcttaccct tttttcgagc cgccgcatga
     3901 tactgcttta cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct
     3961 tacggactca cagggcgtaa actagcgccg tttttttatg tgatgagaag aaaatgaccg
     4021 atctcttttc cagccctgac cacacactcg acgcacttgg cctgcgctgc ccggaaccag
     4081 tcatgatggt gcgcaaaaca gtgcgcaata tgcagccagg tgaaacattg ctgattattg
     4141 ctgacgatcc ggcgacgacc cgcgatatcc ctgggttttg tacctttatg gaacacgaac
     4201 tggttgccaa agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgac
     4261 gggggcagat tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagaggag
     4321 cgaggggacc gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc
     4381 ttaacgcatt cgctgtcacc agcaccgtcg cacccgtatc tgccagcact gccagccata
     4441 gtccggtcat ccctaacagc gtggtgacga ggaagatccc tttcagcccc agcgcaatag
     4501 tgatgttctg gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccagac
     4561 cgcgcaggtg gttatgggtt aatgctgcgt cggcggtttc cagcgccacg tctgtgccgc
     4621 tgcccattgc aatcccgatg gcggcagctt tcatcgctgg cgcgtcgtta ataccgtcac
     4681 cgaccatcgc cagcggcgca tgttgattca gctcggtcac cgctttgact ttatcttccg
     4741 gcaacaggcc cgctttaaac tccagcccca gctccccggc aattgccgcc gctgcgcgtg
     4801 gattatcgcc ggtgaggatc acccctttga cgcccagcgc gttcagttca ctgatggcag
     4861 ttgcagcatc ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac
     4921 gtactaccag cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg
     4981 catcagcggg atgtttcccg gcagcgcata tcaacacgcg ctcaccgtta acctgcgctt
     5041 caatgccaga cccgaccagc gcccgctgtg attcggcggc gggaatggcg agtgcagcaa
     5101 cctgtgcttc gcgcacgatg gcctgcgcca gtggatgcgt cgcgccttgc tcgaccgccg
     5161 ccgccagtgt cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc
     5221 gcggtttacc gacggtcagc gtaccggttt tatcaaacgc cacctgggta acacgaccca
     5281 gctgttccag cgccgctccg cctttaatca acgccccacg acgcgctgcc gccgccagcc
     5341 cggaggtaat cgccgcaggc gttgagataa ctaacgcaca cgggcagcca atcagcagca
     5401 gcgtcagccc tttataaatc cactcctgcc agctggtggc aaacagcagc ggtggcacca
     5461 gcgtcaccag cagagcgacg gccataatcg cgggcgtgta aatacggctg aaacggtcga
     5521 taaaccgctc aatgggtgcg cgacgctctt cggcttcttc gatcagtttc agaatccggt
     5581 caatggcgct ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg
     5641 tggctcctgc aggaacttta tcgcccgttg cgcgctccac cggaatagat tcgccggtca
     5701 gggcgctttc atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgtc
     5761 cacctgcggc gacttcaatc acatcgccag ggcgcaggct gttaatcgcc acctcttccc
     5821 gctcaccgtt acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctgaccc
     5881 cttgacgcgc gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca
     5941 acaccatcgc agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca
     6001 ttaaggtttc aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa
     6061 tcgggtacag cccaaccagc gtggtcgcga taaacgccag ttgcccgaac ggatgattaa
     6121 attgctccaa accccagctg attgccatca tcacgataag cgtaatcagc ggcagattct
     6181 ctttcaggcg cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg
     6241 ctttttgcac cgcagattca acttgtgcac ggatgtcatt gtcggcatca accaccagtt
     6301 tttcggtggc gaacaacacc tgtacctgat tcacgcctgc aagctggcgc acggcatttt
     6361 ctaccttgcg cgcacaggcg gcgcagtcca tgccgctgac tttccagcta tagcgggtgc
     6421 cggagacgtt ttcagagaga gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt
     6481 tggcgttctg taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt
     6541 gattgtcagg agtcgacatg gcatcctccg gttaagtttt ttctcattaa ccgaaggata
     6601 cactctggag tcgactccag agtcaagttt tatcagagat acagcgagcg gacgatgagg
     6661 aagtgaccgg caaagtagca ggccgcagca atcgcgttgt ccgcacggaa gcgtcggcga
     6721 tagtgactac ccagccagac aaagttactg ataaacagca acgaagcgcc gacaaacgca
     6781 gagagtgccg gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc
     6841 agcgtcatgc cgataaacgt gcagataggc caacgatact cttccagccg cgtccagata
     6901 atcgccagta acagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag
     6961 agcgtcatct gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg
     7021 atggcgtaca tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg
     7081 cacagccctg ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt
     7141 aacagcagga gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga
     7201 tacgatgcat ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc
     7261 ttagttcctt gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt
     7321 tagtggcgag ggtggacgga tgacaacacc ataactggca gggtatgctt atttccgcat
     7381 tttccgatga gggataaaga tgagcaaacc acctcttttc tttattgtta tcattggctt
     7441 aatcatcgtc gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga
     7501 tatggcacca ctccagcaaa agctggtgtt ggtgagcaac aagcgggaaa aaccgattaa
     7561 cgatcgccgt tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctatgaggc
     7621 aagcttcaaa ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta
     7681 ccaagccctg acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgttag
     7741 ctttgttggc gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag
     7801 ttcaaacacg ccgaaaagga aaatccgcac cttttcggcg gtggtcatct gcgggccatc
     7861 tttcggtaaa gtggatttca atagcgccag ttgcatacca tgcatcagca cggtaaaaat
     7921 cagcgccacg ttaacgaaaa tattcagcgg gcgcgggaag ggatgcacca ggttgaggat
     7981 taaaaatccc caaacacaga gcattaacaa acgaccaata ttaatcagca tcactttctc
     8041 cttgtgcttc gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta
     8101 acgaccagtt tgccggaacg gtgggcagac cgttttcgac ttcgctttcg acataaatca
     8161 gggtttcgtc agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaaac
     8221 cacggcggaa cggtggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga
     8281 atgacattgc gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct
     8341 taattaactg ctgagaaact gcacgatcca tctcaattaa cgtggccccc gcagcgtagc
     8401 gcgataacgc ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt
     8461 caacaatgac cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc
     8521 gcagacctgg gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc
     8581 ggatttggcc gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga
     8641 taacacatac ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt
     8701 ttttagctgc tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag
     8761 tcgcaaatgg cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa
     8821 gagcagaccc cggaaaaaga gacagaagtt cagaatgaac aaacggttgt agaagaaatc
     8881 gttcaggcgc aagagcctgt gaaggcttct gaacaagccg tcgaagagca gccgcaggcg
     8941 catactgagg ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt
     9001 gctgaaaatg aaaaagcgca gcctgaagcg gaagtcgttg cacaaccgga accggtcgta
     9061 gaagaaacgc cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc
     9121 aacgccgaag aggtttcgcc agaagagtgg caggctgaag cggaaaccgt agagattgtc
     9181 gaagcggcgg aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag
     9241 gcgctggctg ccgaagcagc agaagaggcg gtgatggtgg ttcctccggc agaagaagag
     9301 cagccggtgg aagaaatcgc tcaggagcag gaaaaaccga ccaaagaagg ttttttcgcg
     9361 cgcctgaaac gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg
     9421 ttccgcggta aaaaaatcga cgatgatctg tttgaggagc tggaagagca gcttttgatc
     9481 gccgatgtgg gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc
     9541 cgcaagcagc ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggtgag
     9601 attctggcga aagtcgatga gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg
     9661 atggtgggcg tcaacggtgt gggtaaaacc acgacgattg gtaagctggc acgtcagttt
     9721 gagcagcagg gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agcagcggtt
     9781 gaacagcttc aggtctgggg gcagcgcaac aatattccgg tgattgccca gcataccggg
     9841 gcggattccg cctctgttat cttcgacgcc attcaggcgg ctaaagcgcg taatatcgac
     9901 gtcctgattg ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg
     9961 aagaaaatcg tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg
    10021 actatcgatg ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccatgaagcc
    10081 gttggcttaa ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt
    10141 ttctcggtgg ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt
    10201 gaggatttgc gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat
    10261 taacaatgat tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc
    10321 agggcgttac gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg
    10381 cagggaaaag taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa
    10441 tctggtttag cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc
    10501 gccagattgg catgattttc caggatcacc atctactgat ggaccgtact gtctacgata
    10561 acgtggcgat cccgctgatt atcgccggtg ccagcggtga cgatattcgc cgccgggtgt
    10621 cggcggcact ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt
    10681 ctggcggtga acaacagcgt gttggcattg cccgcgcggt ggtgaacaag cccgcggtac
    10741 tgctggcgga cgaaccgact ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc
    10801 tgtttgaaga gtttaaccgc gtaggggtaa ccgtattgat ggcaacgcac gacatcaacc
    10861 tgatttcacg gcgttcctat cgcatgctca ccctgagcga tggtcacttg catggaggcg
    10921 tgggccatga ataagcgcga tgcaatcaat catattcggc agtttggcgg gcgtcttgat
    10981 cgcttccgta aatcggtcgg cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg
    11041 aaatcctcgc caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat
    11101 gccttccacg gcgcattgca ggatctgaaa agcaaaccgt tcgccacatt tttaacggtg
    11161 atggttatcg ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt
    11221 aaccaggcgg cgacgcagta ttatccgtca ccgcaaatca ctgtttatct gcaaaaaacg
    11281 ctggacgatg acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtagag
    11341 aaagtgaact atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt
    11401 ggtggcgcgc tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg
    11461 aagctcgact ttcaggggac ggagtctctg aatacgctgc gtgatcgtat cacgcagatt
    11521 aacggcattg acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc
    11581 gggctggtcg ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc
    11641 gtcatcggta acagtgtacg cctgagtatc tttgctcgtc gtgactccat taacgtgcag
    11701 aaactgattg gtgcaacgga tggtttcatc ctgcgcccgt tcctgtacgg tggcgcattg
    11761 ctggggtttt ctggcgcatt gttgtcatta attttatcag aaattctggt actgcgattg
    11821 tcatcggcgg ttgcggaagt ggcacaggtt ttcggaacga agtttgatat caatggttta
    11881 tcgttcgatg aatgcctgct gttgctgctg gtatgctcga tgattggctg ggtggcagcg
    11941 tggcttgcca cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata
    12001 ctctttccct gcaatgggtt ccgtagcagg gaaagagacc ccgttgtctc ttcccggtat
    12061 ttcatctcta tgtcacattt tgtgcgtaat ttattcacaa gcctgcattg aacttgtgga
    12121 taaaatcacg gtctgataaa acagtgaatg ataacctcgt tgctcttaag ctctggcaca
    12181 gttgttgcta ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa
    12241 aatgcaaagt ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa
    12301 cgcgtggccg atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca
    12361 tggcgatctg gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat
    12421 tgctcgtaat tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat
    12481 cggcctgatg aaagcagtgc gccgttttaa cccggaagtg ggtgtgcgcc tggtctcctt
    12541 cgccgtccac tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt
    12601 caaagttgcg accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca
    12661 gcgtctgggc tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac
    12721 cagcaaagac gtacgtgaga tggaatcacg tatggcggca caggacatga cctttgacct
    12781 gtcttccgac gacgattccg acagccaacc gatggcaccg gtgctctatc tgcaggataa
    12841 atcatctaac tttgccgacg gcattgaaga tgataactgg gaagagcagg cagcaaaccg
    12901 tctgaccgac gcgatgcagg gtctggacga acgcagccag gacatcatcc gtgcgcgctg
    12961 gctggacgaa gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc
    13021 tgagcgtgtg cgccagctgg aaaagaacgc gatgaaaaaa ctgcgcgctg ctatagaagc
    13081 gtaatttccg ctattaagca gagaaccctg gatgcgagtc cggggttttt gttttttggg
    13141 cctctacaat aatcaatttc ccctccggca aaacgccaat ccccacgcag attgttaata
    13201 aactgtcaaa atagctattc caatatcata aaaatcgggt atgttttagc agagtatgct
    13261 gctaaagcac gggtagtcat gcataaaacg aaataaagtg ctgaacaaca acatcacaac
    13321 acacgtaata accagaagaa tggggattct caggatgaac ataaagggta aagcgttact
    13381 ggcaggatgt atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc
    13441 ggtcgtgggc gcaatgtccg gtccggttgc gcagtacggt gaccaggagt ttaccggcgc
    13501 agagcaggcg gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat
    13561 cgtaaaatat gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt
    13621 taacgacggc attaaatacg tgattggtca cctctgttct tcatcaacgc agcctgcgtc
    13681 tgacatctac gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct
    13741 gaccgcccgt ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc
    13801 gacggcggcg aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga
    13861 caaacagcaa tacggcgaag gtctggcacg agcggtgcag gacggcctga agaaaggcaa
    13921 tgctaacgtg gtgttctttg atggtatcac cgccggggaa aaagatttct caacgctggt
    13981 ggcgcgtctg aaaaaagaga atatcgactt cgtttactac ggcggttatc acccggaaat
    14041 ggggcaaatc ctgcgtcagg cacgcgcggc agggttgaaa actcagttta tggggccgga
    14101 aggcgtggct aacgtctcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt
    14161 gaccaagccg aagaactacg atcaggttcc ggcgaacaaa cccattgttg atgcgatcaa
    14221 agcgaaaaaa caggacccaa gcggcgcatt cgtttggacc acctacgccg cgctgcaatc
    14281 tttgcaggcg ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctgaaagc
    14341 gaactccgtg gataccgtaa tgggaccgct gacctgggat gagaaaggcg atctgaaagg
    14401 ctttgagttc ggtgtgtttg actggcacgc caacggcacg gcaaccgatg cgaagtaaca
    14461 gatagataaa tgaaaatgcc cgatgcgctg aactgccatc gggttttttt gtgtttgtcg
    14521 aaatggttgt agaaagatta aacaatccag gatatgctta atgtacggtg acataccgta
    14581 catattatac ggagcaggag agttcacatg tctgctgtta aaaagcagcg tatcgatctg
    14641 cgtttaactg acgatgacaa aagtatgatt gaggaagcgg cagcgatatc taatcagtcc
    14701 gtcagccaat ttatgttgaa cagcgcttcg cagcgggctg cggaagtgat tgaacagcat
    14761 cggcgggtga tcctcaatga ggaatcctgg acgcgggtga tggatgcgct gagtaatcca
    14821 ccgtcaccag gtgaaaagct aaaacgtgcg gcaaaacgtc ttcagggaat gtaataattt
    14881 atggatgatc tgacgataga gattctgacc gatgatgcag attatgatct acagcgattc
    14941 gactgcggcg aggaagcgtt aaatctcttt ctgacgacac atctcgttcg tcaacatcgc
    15001 aacaaaattc tgcgagcgta tatcctttgt cgcaacactc cagaacgtca ggtgctggga
    15061 tattacacat tatgcggcag ttgttttgaa cgagccgcat tgccctcgaa atcgaaacag
    15121 aaaaaaattc cctacaaaaa tattcccagc gttactcttg ggcgtctggc aattgatcgt
    15181 tcattacagg ggcagggatg gggagcaaca ctggttgctc atgccatgaa agtcgtctgg
    15241 tcagcctctt tagcggtagg tattcacggt ctttttgtcg aggcgctgaa tgaaaaagcc
    15301 catacgtttt ataaatcgct gggctttatc cctttagtcg gggaaaacga aaatgcgtta
    15361 tttttcccaa ccaaatccat tgaactgctt tttacacaga gcgattaacg cttctcccag
    15421 ccgccctgct gtgccgtaaa tcccagtgcc tgcataaacg ccgtcatcac accgcgatct
    15481 tccacgcctg catccgccat ccaccagcat gaaacgccag gattgttacg caaaacctct
    15541 tccagcagat attgccccac accgcgacgt cgagtgactt cccgcacgcg cagggaatcc
    15601 agtgccccct cggtgccgct taaggttacc cgcacggcag caagcaggcg ctcgttaaaa
    15661 cgcgcggcgt agatgcggtg gttatcgtca acctgtaaag aggaagggga atactccggc
    15721 cagatctttt gcaggtcaat ccggtcctgg tcgctaaatt tttctaatcg aatgatggtc
    15781 agcttcatgg gtaacccgtg taaatcacaa aagtggaacc agtgtagcga aataatttaa
    15841 tcggaggctt tctctttttt atttcttttg gcaggtgatt aatttcttaa cagcaataat
    15901 tacaaagtta aaacattaga gaatgaaaaa tacccagcat aatcccctga atggtagtga
    15961 attattccgc cctttgtgcc gttattttat gctgacaaag gcactttttt ctgtttatct
    16021 atcaataaat tcagaatatt atctgttctt aatcgactga aaaataggga ctttaatcgc
    16081 tattatcaca aaatactgcg ctaaaccctt aatcagacag gcaaaaacag tgcagtataa
    16141 aaaaagaaca gtctgatttg ttaacacata aaaacaaagc aacacaacat cacgaatggg
    16201 gatttttgac tatgaaacgg aatgcgaaaa ctatcatcgc agggatgatt gcactggcaa
    16261 tttcacacac cgctttagcc gacgatatta aagttgccgt tgtcggcgcg atgtccggcc
    16321 cgattgccca gtggggcgat atggaattta acggcgcgcg tcaggcgatt aaagacatta
    16381 atgccaaagg gggaattaaa ggcgataagc tggttggcgt agaatatgac gacgcctgcg
    16441 acccgaaaca agccgttgcg gtcgccaaca aaatcgttaa tgacggcatt aaatacgtta
    16501 ttggtcatct gtgttcttct tctacccagc ctgcgtcaga tatctatgaa gacgaaggta
    16561 ttcttatgat ctcgccggga gcgaccaacc cggagctgac ccaacgcggt tatcaacaca
    16621 ttatgcgtac tgccgggctg gactcttccc aggggccaac ggcggcaaaa tacattcttg
    16681 agacggtgaa gccccagcgc atcgccatca ttcacgacaa acaacagtat ggcgaagggc
    16741 tggcgcgttc ggtgcaggac gggctgaaag cggctaacgc caacgtcgtc ttcttcgacg
    16801 gtattaccgc cggggagaaa gatttctccg cgctgatcgc ccgcctgaaa aaagaaaaca
    16861 tcgacttcgt ttactacggc ggttactacc cggaaatggg gcagatgctg cgccaggccc
    16921 gttccgttgg cctgaaaacc cagtttatgg ggccggaagg tgtgggtaat gcgtcgttgt
    16981 cgaacattgc cggtgatgcc gccgaaggca tgttggtcac tatgccaaaa cgctatgacc
    17041 aggatccggc aaaccagggc atcgttgatg cgctgaaagc agacaagaaa gatccgtccg
    17101 ggccttatgt ctggatcacc tacgcggcgg tgcaatctct ggcaactgcc cttgaacgta
    17161 ccggcagcga tgagccgctg gcgctggtga aagatttaaa agctaacggt gcaaacaccg
    17221 tgattgggcc gctgaactgg gatgaaaaag gcgatcttaa gggatttgat tttggtgtct
    17281 tccagtggca cgccgacggt tcatccacgg cagccaagtg aaaatcccac cgcccgtgaa
    17341 atgcgggcgg gtttagaaag gttaccttat gtctgagcag tttttgtatt tcttgcagca
    17401 gatgtttaac ggcgtcacgc tgggcagtac ttacgcgctg attgccatcg gctacaccat
    17461 ggtttacggc attatcggca tgatcaactt cgcccacggc gaggtttaca tgatcggcag
    17521 ctacgtctca tttatgatca tcgccgcact gatgatgatg ggcattgata ccggctggct
    17581 gctggtagcg gcgggattcg tcggcgcaat cgtcattgcc agcgcctacg gctggagtat
    17641 cgaacgggtg gcttaccgcc cggtgcgtaa ctctaagcgc ctgattgcac tcatctcggc
    17701 aatcggtatg tccatcttcc tgcaaaacta cgtcagcctg actgaaggtt cgcgcgacgt
    17761 ggcgctgccc agcttgttta atggtcagtg ggtagtgggt catagcgaaa acttctctgc
    17821 ctctattacc accatgcagg cggtgatctg gattgtcacc ttcctcgcca tgctggcgct
    17881 gacgattttc attcgttatt cccgtatggg gcgcgcctgc cgtgcctgcg ccgaagatct
    17941 gaaaatggcg agcctgcttg gcattaacac cgaccgggtg attgctttga cctttgtgat
    18001 tggcgcggcg atggcggcgg tggcgggcgt actgctcggt cagttctacg gcgtcattaa
    18061 cccctatatc ggctttatgg ccgggatgaa agcctttacc gctgcggtgc tcggtgggat
    18121 tggcagcatt ccgggagcga tgattggcgg gctgattctg gggattgcgg aggcgctctc
    18181 ttctgcctac ctgagtacgg aatataaaga tgtggtctca ttcgccctgc tgattctggt
    18241 gctgctggtg atgccgaccg gtattctggg tcgcccggag gtagagaaag tatgaaaccg
    18301 atgcatattg caatggcgtt gctctctgcc gcgatgtttt ttgtcctggc gggcgtcttt
    18361 atgggcgtac aactggagct ggatggcacc aaactggtgg tcgacacggc ttcagacatc
    18421 cgttggcagt gggtgtttat cggcacggcg gtggtctttt tcttccagct tttgcgaccg
    18481 gctttccaga aagggttgaa aagcgtttcc ggaccgaagt ttattctgcc cgccattgat
    18541 ggctccacgg tgaagcaaaa gctgtttctc gtggcgctgc tggtgcttgc ggtggcgtgg
    18601 ccgtttatgg tctcacgcgg gacggtggat atcgccaccc tgaccatgat ctacattatc
    18661 ctcggactcg ggctgaacgt ggtggtaggg ctttctggtc tgctggtgct ggggtacggc
    18721 ggcttttacg ccatcggcgc ttataccttt gcgctgctca atcactatta cggcttgggc
    18781 ttctggacct gtctgccgat agccgggttg atggcggcgg cggcgggctt cctgctcggc
    18841 ttcccggtgc ttcgcttgcg cggtgactat ctggcgatcg ttaccctcgg tttcggcgaa
    18901 attgtgcgca tattgctact caataacacc gaaattaccg gcggtccgaa cggtatcagt
    18961 cagatcccga aaccgacttt tttcggcctt gagttcagcc gtaccgctcg tgaaggcggc
    19021 tgggacacgt tcagtaattt ctttggcctg aaatacgatc cctccgatcg tgtcattttc
    19081 ctctacctgg tggcgttgct gctggtggtg ctaagcctgt ttgtcattaa ccgcctgctg
    19141 cggatgccgc tgggtcgcgc ctgggaagcg ttgcgtgaag atgaaatcgc ctgccgttcg
    19201 ctgggcttaa gcccgcgtcg tatcaagctg actgccttta ccatcagcgc cgcgtttgcc
    19261 ggttttgccg gaacgctgtt tgctgcgcgt cagggctttg tcagcccgga atccttcacc
    19321 tttgccgaat cggcgtttgt gctggcgata gtggtgctcg gcggtatggg ctcgcaattt
    19381 gcggtaattc tggcggcaat tttgctggtg gtgtcgcgcg agttgatgcg tgatttcaac
    19441 gaatacagca tgttaatgct cggtggtttg atggtgctga tgatgatctg gcgtccgcag
    19501 ggcttgctgc ccatgacgcg cccgcaactg aagctgaaaa acggcgcagc gaaaggagag
    19561 caggcatgag tcagccatta ttatctgtta acggcctgat gatgcgcttc ggcggcctgc
    19621 tggctgtgaa taacgtcaat cttgaattgt acccgcagga gatcgtctcg ttaatcggtc
    19681 ctaacggtgc cggaaaaacc acggtcttta actgcctgac cggattctac aaacccaccg
    19741 gcggcactat tttactgcgc gatcagcatc tggagggttt gccggggcag caaatcgccc
    19801 gcatgggcgt ggtgcgtacc ttccagcatg tgcgtctgtt ccgcgaaatg acggtaattg
    19861 aaaacctgct ggtggcgcag catcagcaac tgaaaaccgg gctgttctct ggcctgttga
    19921 aaacgccttc tttccgtcgc gcccaaagtg aagcgctcga ccgcgccgcc acctggcttg
    19981 agcgcattgg tttgctggaa cacgccaacc gtcaggcgag taacctggcc tacggtgacc
    20041 agcgccgtct ggagattgcc cgctgcatgg tgacgcagcc ggagatttta atgctcgacg
    20101 aacctgcggc aggtcttaac ccgaaagaaa cgaaagagct ggatgagctg attgccgaac
    20161 tgcgcaatca tcacaacacc actatcttgt tgattgaaca cgatatgaag ctggtgatgg
    20221 gaatttcgga ccgaatttac gtggtcaatc aggggacgcc gctggcaaac ggtacgccgg
    20281 agcaaatccg taataacccg gacgtgatcc gtgcctattt aggtgaggca taagatggaa
    20341 aaagtcatgt tgtcctttga caaagtcagc gcccactacg gcaaaatcca ggcgctgcat
    20401 gaggtgagcc tgcatatcaa tcagggcgag attgtcacgc tgattggcgc gaacggggcg
    20461 gggaaaacca ccttgctcgg cacgttatgc ggcgatccgc gcgccaccag cgggcgaatt
    20521 gtgtttgatg ataaagacat taccgactgg cagacggcga aaatcatgcg cgaagcggta
    20581 gcgattgtcc cggaagggcg tcgcgtcttc tcgcggatga cggtggaaga gaacctggcg
    20641 atgggcggct tttttgccga acgtgatcag tttcaggagc gcataaagtg ggtgtatgag
    20701 ctgtttccac gtcttcatga gcgtcgtgtt cagcgggcag gcaccatgtc cggcggtgaa
    20761 cagcagatgc tggcgattgg tcgcgcgctg atgagcaatc cgcgtttgtt gttgcttgat
    20821 gagccatcgc tcggtcttgc gccgattatc atccagcaaa ttttcgacac catcgagcag
    20881 ctgcgcgagc aggggatgac tatcttcctc gtcgagcaga acgccaacca ggcgctgaag
    20941 ctcgctgatc gcggctacgt gctggaaaac ggccatgttg tgctctccga tactggtgat
    21001 gcgctgctgg cgaacgaagc agtgagaagt gcgtatttag gcgggtaaat tatcgataac
    21061 ccggcagcta agttctgccg ggttattcgt ccgtatacag acttgtttca actgaccggg
    21121 aggattaaaa ccagaccact tatttgccac ggaaaggttc cctcccatgc attatgccgt
    21181 tgttgtcatg cttgaactgc tttgctgtat tcacgcttac cgtaccggac aagagcggta
    21241 ctggattttt atcattttct gttttccggt gattggctgc gtggcgtatt tcgtcatagt
    21301 gatgctgcct gaaacgggcg ctgaccgtca cgggcatact ttgctgatgc gcctgcagga
    21361 caaactcaac cctgagcgcc acctgcgcaa attaaccgaa gaactggcga ttgccgagac
    21421 gaatcaaaac cattatgcgt tggctaatga actggcgcgt ctggggcgtt atcacgaagc
    21481 tgttccccat tatcagcagg cgttaagcgg gatattcgcc catgaagcgg caatgatgct
    21541 aagcctggcg caggcacagt ttgctattca ggagttcgcc gcctgtcagc agacgctgga
    21601 agatgtgatg cgttataacc ctgattttca gtcggcagac gggcatttgt tatttgccag
    21661 aacgttagcc gcacaagaaa aatacgctga tgcggaaagc gagtttgaag tattgatttc
    21721 ttattacccc ggcccgcagg cgcgtattta ctatgcggaa atgttggcga aaatgagtcg
    21781 gttacgtgaa gccaacgagc agtatgttgc cgtggttgat acggctaaac gctcaagacc
    21841 tcattataga aaacaccacc gggaatggat taagaccgcc aatgagcggc tgaagcagag
    21901 tgtggtgcaa tgaaaatctt ctttgccgtt agaccagtgt cgcggcaatc ttttgttaaa
    21961 attgaaaaat gtagctctca tcgggcagca aaattggacg cagaatttgc tgccattttt
    22021 gctcttcatg gtaaggagtt ctggcgatta gctggccgtt aatcgcctct tttcagtgca
    22081 ttctcgggat atccgtgccc catctcccat tttccctcac ccgtaccgtc accgccttgt
    22141 catctttctg acaccttact atcttacaaa tgtaacaaaa aagttatttt tctgtaattc
    22201 gagcatgtca tgttaccccg cgagcataaa acgcgtgaat tcgcgcattc ggtacaacaa
    22261 gagagataaa cgatgaaacc gttacattat acagcttcag cactggcgct cggactggcg
    22321 ttaatgggga atgcacaggc agtgacgacc attccgttct ggcattctat ggaaggggaa
    22381 ctgggtaaag aggtggattc tctggcccaa cgttttaacg ccgaaaaccc ggattacaaa
    22441 attgtaccga cctataaagg caattacgaa cagaatttaa gcgcggggat tgccgcattt
    22501 cgtaccggca atgctccggc tattttgcag gtttatgaag ttggcaccgc caccatgatg
    22561 gcgtcgaaag ccattaaacc ggtttatgac gtgtttaaag aggcggggat tcaattcgat
    22621 gagtcgcagt ttgtgccgac ggtttccggt tactactccg acagcaaaac gggccactta
    22681 ctctcccagc cattcaacag ctcgactccc gttctctatt acaacaaaga cgccttcaag
    22741 aaagcggggt tagacccgga acaaccgccg aaaacctggc aggatctggc ggactatgcc
    22801 gcaaaactaa aagcctccgg catgaagtgc ggctacgcca gcggctggca gggctggatc
    22861 caactggaaa actttagcgc ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt
    22921 gacggcacgg atgcggtgct ggagttcaac aagccggagc aggtgaaaca catcgccatg
    22981 ctcgaagaga tgaacaagaa gggcgatttc agctacgtcg ggcgtaagga tgaatccacc
    23041 gagaagttct ataacggtga ttgcgctatg accaccgcct cttccggttc tctcgctaat
    23101 attcgcgagt acgccaaatt caactatggc gtaggcatga tgccttacga cgccgatgcg
    23161 aaagatgcac cgcaaaacgc cattatcggc ggggccagcc tgtgggtgat gcagggcaaa
    23221 gataaagaaa cgtataccgg cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac
    23281 gctgccgagt ggcatcagaa aaccggttat ctgccaatca ccaaagcagc gtatgacctg
    23341 acccgtgagc agggctttta tgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg
    23401 ctgaataagc cgccgttgcc gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc
    23461 cgcgtgattg tggatgaaga gctggagagc gtgtggaccg gtaagaagac accacagcag
    23521 gcactggata ccgccgttga gcgtggaaat cagttgctgc gccgctttga gaaatcgacg
    23581 aagtcttaat cagtgtaatg tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag
    23641 gccggataag cgaagcgcat ccggcacagt tcaggaatta accgtaatgt catcatcccg
    23701 tccggtgttc cgctcgcgct ggctgcctta tctgctggtc gcgccgcagc tcatcatcac
    23761 cgttatcttt tttatctggc ctgcgggcga agcgttgtgg tactcgctgc aaagcgtcga
    23821 tccgtttggt ttctccagcc ggtttgtcgg cctggataac ttcgtcgcgc tgtttcatga
    23881 cagctactat atcgactcct tctggacgac gataaaattc agcacctttg tcaccgtcag
    23941 cggtttgctg gtatcgctgt tctttgcggc gctggtggag tacatcgtgc gcggcagtcg
    24001 tttctatcaa accttgatgc tgctgccgta tgccgtggct cccgccgttg ccgccgtatt
    24061 gtggatcttc ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg ctgagttcgg
    24121 ctacgactgg aaccacgcgc aaaacagcgg tcaggcaatg tttctggtgg tgtttgcctc
    24181 agtatggaag caaatcagct acaacttcct gttcttctat gccgcgctgc aatccattcc
    24241 ccgttcgttg atcgaagccg cagccatcga cggtgcaggg ccgattcgcc gcttctttaa
    24301 gattgcgctg ccgcttatcg ccccggtgag tttcttcctg ctggtagtga acctggtgta
    24361 tgccttcttc gacaccttcc cggtgatcga cgccgccacg tccggcgggc cggttcaggc
    24421 caccactacg ctgatttata agatctaccg cgaaggcttt accggactgg atctggcttc
    24481 gtctgccgca cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg tgatgcagtt
    24541 ccgctatgtt gaaagcaagg tgcgttacca atgattgaga accgtccgtg gctgacgata
    24601 ttcagccata ccatgctgat cctcgggatc gcggtgatcc tattcccgct gtacgtggcg
    24661 tttgtcgcgg cgacgctgga taaacaggcc gtctacgccg cgccgatgac gctcatcccc
    24721 ggcacgcatc tgctggaaaa catccacaac atctgggtaa acggggtagg cataaatagt
    24781 gcgccgttct ggcggatgtt gcttaacagc ttcgtgatgg cgttcagcat tacgctcggc
    24841 aaaattaccg tctcgatgct ctcggcattc gccattgtct ggtttcgttt tccgctgcgt
    24901 aacctctttt tctggatgat ttttatcacc ctgatgttgc cggttgaagt acgtatcttc
    24961 ccgacggtgg aagtcatcgc caacctgaag atgctcgaca gctacgcagg tttaacgctg
    25021 ccgctgatgg cctcggcgac cgcgacgttt cttttccgcc agttctttat gaccctaccg
    25081 gatgaactgg tggaagccgc gcggatcgac ggcgcgtcgc caatgcgctt cttttgcgac
    25141 atcgtttttc cgttgtccaa aactaatctg gcggcgttgt ttgtgatcac ctttatttac
    25201 ggctggaacc agtatttgtg gccgttgttg attattaccg acgtggatct cggcaccacc
    25261 gtggcaggga tcaaagggat gatcgccact ggcgaaggca ccacagaatg gaactcagtg
    25321 atggcggcga tgttgttaac cctgatccca ccagtagtga ttgttttagt gatgcagcgt
    25381 gccttcgtgc gcggcctggt cgatagtgag aaataagatg gcaggactga aattacaggc
    25441 agtaaccaaa agctgggatg gcaaaaccca ggtgattaaa ccgctgaccc ttgatgtggc
    25501 ggatggcgaa tttatcgtga tggtcgggcc gtcaggctgc gggaaatcga cgctgctgcg
    25561 catggttgcc gggctggagc gggtgacgga aggcgatatc tgtatcaacg accagcgggt
    25621 gaccgaaatg gagccgaaag atcgcgggat tgcgatggtg ttccagaact acgcgcttta
    25681 tccgcatatg agtgttgaag aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa
    25741 gcagcaaatt gccgagcgcg ttaaagaggc ggcgcgcatt ctggaactgg acggtctgct
    25801 taagcgccgc ccgcgcgagc tttccggcgg tcagcgtcag cgtgtggcga tgggccgggc
    25861 gattgtgcgc gatccggcgg tgttcctgtt tgatgagcca ctctctaacc tcgatgccaa
    25921 gctgcgcgta cagatgcgtc ttgaactgca acagctgcac cgtcgcctga aaacgacttc
    25981 actctacgtt actcacgatc aggttgaggc gatgaccctc gcccagcgag taatggtgat
    26041 gaacggcggc gttgccgaac agattggcac accagttgaa gtctacgaaa agcccgccag
    26101 cctgtttgtg gcgagtttta ttggcagccc ggcgatgaat ctgctgacag gccgcgtgaa
    26161 taacgaaggc acgcacttcg agctggacgg cggcattgcg ctgccgctaa acggtggcta
    26221 ccgtcagtat gcagggcgta aaatgactct cggcattcgc ccggaacata tcgcgctaag
    26281 ctcgcaggca gaaggcggcg tgccgctggt gatggacacg ctggagatcc tcggcgcaga
    26341 taacctggcg cacggacgct ggggcgaaca gaagctggtg gtacggctgg cgcatcagga
    26401 gcgcccgacg gcaggcagca cgctgtggct gcatctgccg gaaaatcagc tacatctttt
    26461 tgatggtgaa acaggacaac gagtatgagt aactggcctt atccccgcat cgtcgcccac
    26521 cgtggcggcg gtaaactggc cccggaaaac accctggcgg caatcgacgt cggggcaaaa
    26581 tacggtcata agatgatcga atttgacgcg aagttgtcga aagatggcga aatcttcctg
    26641 ctccatgacg acaatctcga acgtaccagc aacggctggg gcgtcgcggg tgaactgaac
    26701 tggcaggatt tactacgtgt ggatgcaggc agttggtacg gcaaagcgtt taaaggtgag
    26761 ccgctgccgt tgctttcgca ggtggcggaa cgctgtcgcg aacatgggat gatggcgaat
    26821 atcgaaatca aacccaccac tggcactggg ccattaacgg gcaaaatggt ggcgctggcg
    26881 gcacgcgagc tgtgggccgg tatgacgccg ccgctactgt catcgtttga gattgatgct
    26941 ttagaagccg cacaacaggc agtaccagaa ctgccgcgcg gtttgttgct ggatgagtgg
    27001 cgcgacgact ggcgagaact gaccgcgcgg ctgggctgcg tctctattca tctcaatcat
    27061 aagttgctcg ataaagcgcg agtgatgcag ttgaaagacg ccggattacg gattctggtt
    27121 tataccgtca ataaacccca gcgcgcggca gagttgctgc gctggggcgt ggattgcatc
    27181 tgtaccgatg cgattgatgt gattggtccg aactttacgg cccaatagtt ttcaacggta
    27241 tatcaggctg cggcgtgccg ggctgattca gcatgtcgcc gtttctcctc tccggcaaca
    27301 tatgctgctg attcaacgaa ctatccggat tacggttgct gtttaacatc ccgccattgg
    27361 tgttgggcag catttgctgc cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc
    27421 gctgagaatt attgtttatc tggttttcta aatgctgttg ttgcaactga gtttgcgttt
    27481 tcagttgctg attcagcatc cctttttgct ggatttgctg cgtttgcatc tgggtttgca
    27541 tccgctgctg actggggatc tgataccccg gctggttagg gttgttcaga gtattaatgg
    27601 gctgtgcaaa gccgacaaac ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt
    27661 atcctcctct gaagatattc tttaagttta ctcctttcct gcaatcatca ggattaattc
    27721 agagttatat accaggctta gctggggttg ccccttaatc tctggagaat aacgatgata
    27781 aaaccgacgt ttttacgccg ggtggccatt gctgctctgt tcacaggaag ttgttttagt
    27841 acggtagccg cgccacctgc gccgtctcct gtttcttacg gcgtggagga agatgtcttt
    27901 catccggtac gcgcgaaaca ggggatggtg gcatctgtag atgctactgc cactcaggtg
    27961 ggggtggata tcctcaagga gggcgggaat gccgttgatg ccgccgtggc tgtgggctac
    28021 gcgctggcgg taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgctgatc
    28081 cgctcgaaaa atggcaatac caccgctatc gatttccgcg aaatggcacc cgccaaagcg
    28141 acccgcgata tgttccttga tgaccagggc aacccggaca gcaaaaaatc actcacttcg
    28201 catctggctt ccggcacgcc cggtacggtc gctggtttct cgctggcgtt ggataaatac
    28261 ggcacaatgc cgttgaacaa agtggtgcag ccagcgttta aactggcacg cgatggtttt
    28321 atcgttaacg acgagctggc tgacgatctc aaaacctacg ggagcgaagt gctgccgaat
    28381 cacgaaaaca gcaaggctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacaag
    28441 ctggtgcagg cgaacctggc aaagagcctg gagatgattg ccgaaaacgg cccggacgaa
    28501 ttctacaaag gcacgatagc ggaacagatt gcccaggaga tgcagaaaaa cggtggcctg
    28561 atcactaaag aagatttagc ggcgtataaa gcggtcgaac gcacaccgat aagcggcgat
    28621 tatcgcgggt atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta
    28681 caaatcctca atattctgga aaacttcgat atgaagaaat acggttttgg cagcgccgac
    28741 gcgatgcaga tcatggcaga agcggagaaa tatgcctacg ctgaccgctc ggaatatctt
    28801 ggcgacccgg attttgtgaa agtcccgtgg caggcgctga ccaataaagc ctatgccaaa
    28861 tctattgccg agcaaatcga tatcaataaa gcgaaaccgt ccagcgagat tcgccccggc
    28921 aagcttgcgc cttatgagag taatcaaact acccattatt cggtggtgga taaagacggt
    28981 aacgcggtgg cggtgaccta tacgctgaac accaccttcg gtacgggtat tgtcgcgggc
    29041 gagagcggta ttttgctcaa taaccaaatg gatgatttct ctgccaaacc gggcgtaccg
    29101 aacgtttacg ggctggtggg cggtgatgcc aacgccgtcg ggccgaacaa acgcccgctg
    29161 tcatcgatgt caccaaccat tgtggtgaaa gacggtaaaa cctggctggt caccggtagc
    29221 cctggcggga gcctgatcat cactacagtg cttcaaatgg tggtgaatag catcgattat
    29281 ggcatgaacg tcgccgaagc gaccaatgcg ccgcgtttcc accatcagtg gttgccggac
    29341 gagctgcgtg tcgaaaaagg gtttagcccg gatacgctca agctgctgga agcaaaaggt
    29401 cagaaagtgg cgctgaaaga ggcgatgggc agtacacaaa gcattatggt tgggccggac
    29461 ggtgagttgt acggcgcatc cgacccgcgc tcggtggatg atttaacggc ggggtactaa
    29521 ggttagcggc cctcttcgtg ggaagagggc tgttttcttg aacaagataa attagtgtct
    29581 ggattgcttt tactattgct ccgtaatata taatcataat tatcctgaat gcttttaagg
    29641 ggcttaggtt atgctttctt ttcaagagta tgcagaatag tctttcattt accgtgaaaa
    29701 taggacgtgc gataattact tcctgatatt caggaaag
//
LOCUS       DAZGXS010000050        28765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGXS010000050 DAZGXS010000000
VERSION     DAZGXS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            108..1061
                     /gene="ybhD"
                     /locus_tag="ACHKG5_002308"
     CDS             108..1061
                     /gene="ybhD"
                     /locus_tag="ACHKG5_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0868410.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1102..2097)
                     /gene="pgl"
                     /locus_tag="ACHKG5_002309"
     CDS             complement(1102..2097)
                     /gene="pgl"
                     /locus_tag="ACHKG5_002309"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0868411.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2252..3070
                     /gene="ybhA"
                     /locus_tag="ACHKG5_002310"
     CDS             2252..3070
                     /gene="ybhA"
                     /locus_tag="ACHKG5_002310"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0868412.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3071..4129)
                     /gene="modC"
                     /locus_tag="ACHKG5_002311"
     CDS             complement(3071..4129)
                     /gene="modC"
                     /locus_tag="ACHKG5_002311"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0868413.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4132..4821)
                     /gene="modB"
                     /locus_tag="ACHKG5_002312"
     CDS             complement(4132..4821)
                     /gene="modB"
                     /locus_tag="ACHKG5_002312"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0868414.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4821..5594)
                     /gene="modA"
                     /locus_tag="ACHKG5_002313"
     CDS             complement(4821..5594)
                     /gene="modA"
                     /locus_tag="ACHKG5_002313"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0868415.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5761..5910)
                     /gene="acrZ"
                     /locus_tag="ACHKG5_002314"
     CDS             complement(5761..5910)
                     /gene="acrZ"
                     /locus_tag="ACHKG5_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0868416.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6039..6827
                     /gene="modE"
                     /locus_tag="ACHKG5_002315"
     CDS             6039..6827
                     /gene="modE"
                     /locus_tag="ACHKG5_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0868417.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6895..8367
                     /gene="modF"
                     /locus_tag="ACHKG5_002316"
     CDS             6895..8367
                     /gene="modF"
                     /locus_tag="ACHKG5_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0868418.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            8629..9645
                     /gene="galE"
                     /locus_tag="ACHKG5_002317"
     CDS             8629..9645
                     /gene="galE"
                     /locus_tag="ACHKG5_002317"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0868419.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9655..10701
                     /gene="galT"
                     /locus_tag="ACHKG5_002318"
     CDS             9655..10701
                     /gene="galT"
                     /locus_tag="ACHKG5_002318"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0868420.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10705..11853
                     /gene="galK"
                     /locus_tag="ACHKG5_002319"
     CDS             10705..11853
                     /gene="galK"
                     /locus_tag="ACHKG5_002319"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0868421.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11847..12887
                     /gene="galM"
                     /locus_tag="ACHKG5_002320"
     CDS             11847..12887
                     /gene="galM"
                     /locus_tag="ACHKG5_002320"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0868422.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            13089..13841
                     /gene="gpmA"
                     /locus_tag="ACHKG5_002321"
     CDS             13089..13841
                     /gene="gpmA"
                     /locus_tag="ACHKG5_002321"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0868423.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(14007..15059)
                     /gene="aroG"
                     /locus_tag="ACHKG5_002322"
     CDS             complement(14007..15059)
                     /gene="aroG"
                     /locus_tag="ACHKG5_002322"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0868424.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15375..15755
                     /gene="ybgS"
                     /locus_tag="ACHKG5_002323"
     CDS             15375..15755
                     /gene="ybgS"
                     /locus_tag="ACHKG5_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0868425.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15869..16810
                     /gene="zitB"
                     /locus_tag="ACHKG5_002324"
     CDS             15869..16810
                     /gene="zitB"
                     /locus_tag="ACHKG5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0868426.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16807..17526)
                     /gene="pnuC"
                     /locus_tag="ACHKG5_002325"
     CDS             complement(16807..17526)
                     /gene="pnuC"
                     /locus_tag="ACHKG5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0868427.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17564..18607)
                     /gene="nadA"
                     /locus_tag="ACHKG5_002326"
     CDS             complement(17564..18607)
                     /gene="nadA"
                     /locus_tag="ACHKG5_002326"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0868428.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18885..18960)
                     /locus_tag="ACHKG5_002327"
     tRNA            complement(18885..18960)
                     /locus_tag="ACHKG5_002327"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18925..18927),aa:Lys,seq:ttt)
     gene            complement(19107..19182)
                     /locus_tag="ACHKG5_002328"
     tRNA            complement(19107..19182)
                     /locus_tag="ACHKG5_002328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19147..19149),aa:Lys,seq:ttt)
     gene            complement(19186..19261)
                     /locus_tag="ACHKG5_002329"
     tRNA            complement(19186..19261)
                     /locus_tag="ACHKG5_002329"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19226..19228),aa:Val,seq:tac)
     gene            complement(19313..19388)
                     /locus_tag="ACHKG5_002330"
     tRNA            complement(19313..19388)
                     /locus_tag="ACHKG5_002330"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19353..19355),aa:Lys,seq:ttt)
     gene            complement(19391..19466)
                     /locus_tag="ACHKG5_002331"
     tRNA            complement(19391..19466)
                     /locus_tag="ACHKG5_002331"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19431..19433),aa:Val,seq:tac)
     gene            complement(19502..19577)
                     /locus_tag="ACHKG5_002332"
     tRNA            complement(19502..19577)
                     /locus_tag="ACHKG5_002332"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19542..19544),aa:Lys,seq:ttt)
     gene            complement(19742..20533)
                     /gene="cpoB"
                     /locus_tag="ACHKG5_002333"
     CDS             complement(19742..20533)
                     /gene="cpoB"
                     /locus_tag="ACHKG5_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0868429.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20543..21064)
                     /gene="pal"
                     /locus_tag="ACHKG5_002334"
     CDS             complement(20543..21064)
                     /gene="pal"
                     /locus_tag="ACHKG5_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0868430.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21099..22391)
                     /gene="tolB"
                     /locus_tag="ACHKG5_002335"
     CDS             complement(21099..22391)
                     /gene="tolB"
                     /locus_tag="ACHKG5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0868431.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDVVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22524..23738)
                     /gene="tolA"
                     /locus_tag="ACHKG5_002336"
     CDS             complement(22524..23738)
                     /gene="tolA"
                     /locus_tag="ACHKG5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0868432.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            complement(23803..24231)
                     /gene="tolR"
                     /locus_tag="ACHKG5_002337"
     CDS             complement(23803..24231)
                     /gene="tolR"
                     /locus_tag="ACHKG5_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0868433.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24235..24927)
                     /gene="tolQ"
                     /locus_tag="ACHKG5_002338"
     CDS             complement(24235..24927)
                     /gene="tolQ"
                     /locus_tag="ACHKG5_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0868434.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24924..25328)
                     /gene="ybgC"
                     /locus_tag="ACHKG5_002339"
     CDS             complement(24924..25328)
                     /gene="ybgC"
                     /locus_tag="ACHKG5_002339"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0868435.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25478..25771)
                     /gene="ybgE"
                     /locus_tag="ACHKG5_002340"
     CDS             complement(25478..25771)
                     /gene="ybgE"
                     /locus_tag="ACHKG5_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0868436.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25771..25884)
                     /gene="cydX"
                     /locus_tag="ACHKG5_002341"
     CDS             complement(25771..25884)
                     /gene="cydX"
                     /locus_tag="ACHKG5_002341"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0868437.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25899..27038)
                     /gene="cydB"
                     /locus_tag="ACHKG5_002342"
     CDS             complement(25899..27038)
                     /gene="cydB"
                     /locus_tag="ACHKG5_002342"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0868438.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27054..28622)
                     /gene="cydA"
                     /locus_tag="ACHKG5_002343"
     CDS             complement(27054..28622)
                     /gene="cydA"
                     /locus_tag="ACHKG5_002343"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0868439.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7180 a         7615 c         7084 g         6886 t
ORIGIN      
        1 gctcaacgat taataacatt ttttcattaa tttaagttgg ctaaatgcag ttaaaatttt
       61 taacggccag ccacccaaaa tcgtcgcttc catttcccgg agtaataatg aagcatgaat
      121 tatcaagtat gaaggcattt gtcatactgg cagagtccag ttcatttaac aatgccgcta
      181 aattactcaa tattacgcaa cctgcattaa cacgaagaat aaaaaagatg gaggaagatt
      241 tacatattca actatttgag cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa
      301 ggttgctccc agaggcgcgg gaattaataa agaaattcga tgaaacgctt tttaatattc
      361 gtgatatgaa tgcttatcat cgtggtatgg tgacgttagc gtgtattccg accgcagtat
      421 tttatttttt accgctggca atcggtaaat ttaatgagct gtatcccaat attaaagtgc
      481 ggattctgga acaaggcacg aataattgca tggaatcggt gctgtgtaac gaatcagact
      541 ttggcataaa catgaacaac gtcacaaatt catccattga ttttacccca ctggtcaacg
      601 agccgttcgt gctggcctgt cggcgtgacc atccattggc taaaaagcag cttgtagaat
      661 ggcaggagtt agtgggttac aaaatgattg gcgtacgttc ctccagtggc aaccgattgt
      721 tgatagaaca acagctagcc gacaagccct ggaagctgga ctggttttac gaagtgcgtc
      781 atctttcgac gtcattagga ctggtagagg cgggactggg aatttcggcg cttcctggtc
      841 tggcaatgcc ccatgcgccc tattcttcta ttattggtat cccactggtg gaacccgtta
      901 tacgtcggac attggggatt attcgccgca aagatgccgt gctttctccg gcagcagaac
      961 gcttttttgc cttgctgatt aatttgtgga ctgacgataa agacaatttg tggaccaata
     1021 tcgtcgaacg ccagagacat gcgttacagg agataggtta atacaccggc gcaggagaga
     1081 ctgcgccggg taaatcagcg gttagtgtgc gttaaccacc acccacattg gtccctgccc
     1141 gaccgcgtag cggccttttt catgcagtag cccctgctca ccaacaattt cgtataccga
     1201 gatgtggtgc gatttttgcc cggcggcaat cagatactta ccacggtgat cgatattaaa
     1261 gccgcgcggc tgggtttcag ttggctggaa gccctcttta ctcaacacgc tgccatcttc
     1321 cgaaacgctg aagacagtaa tcaggctggc ggtacggtca caggcatata agtgacgacc
     1381 atccggggta atatgaatat cagccgccca acgggtgtcg gagaagtttt ccggcatcat
     1441 atccagcgtc tggacacatt cgatattgcc gtgcggatct ttcagttccc agacatccac
     1501 tgagctgttt aactcattga cgcaatatgc atattgttcg tttggatgga ataccatatg
     1561 acgcgggccg gccccttcaa cggtagtcac ttccgcaggg tcctgcgcca ccagatgacc
     1621 atcatcgctg accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa cccacagcgt
     1681 acggttgtcc ggtgagatat tggcggaatg gcaaccgtcc agcccctcga ccacatcgac
     1741 gacgccaact ggcaggccat cttccagacg cgttacgctc acgttacccg cattgtaaga
     1801 acctacaaag acaaactgcc cctggtgatc ggtggaaata tgcgtcggac tacccggcag
     1861 cgcagactct gcggcaaagg tcagtgcgcc atcgtccggg gcgatacgat acgccaggac
     1921 gcgaaactca gggcgaacac caacatagag ataacgtttg tccgggctga ccaccatcgg
     1981 ctgcacctgc cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt catgattcag
     2041 gttccagacg tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa
     2101 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
     2161 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagaa
     2221 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
     2281 agacggcacc ttattgaccc cgaaaaagac cctgctacct tcatcgatag aagccctggc
     2341 ccgcgctcgc gaagcaggtt atcaattaat catcgtcaca ggtcgccatc acgtcgctat
     2401 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     2461 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     2521 cctacaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     2581 cgagatggtt tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     2641 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacggcgca
     2701 acaagttaac gccgtatgga agttcgccct cacgcacgat gacctgccgc aattgcagca
     2761 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     2821 ggttgatatt gcacgcggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     2881 gcaaggctgg tcgatggaaa acgtcgtggc gttcggcgat aactttaatg atatcagtat
     2941 gctggaggcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     3001 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     3061 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     3121 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     3181 cgacttccag ttccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     3241 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     3301 cctgaatgcg gatacgtagc gcagcttgca gcggttcgtc cagcttattg acccacaaat
     3361 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agtaccgtca
     3421 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactacccc
     3481 agacttcttc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     3541 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcggg atgttgattt
     3601 cccgcgtcag acgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     3661 ccagcggttc atccagcaac agcaattctg gtgccgtcag caaagcccgc ccaatcgcta
     3721 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     3781 ctaaaagcgc caccagctta tcgaactgat cgaccatgct tttcgccatg ccgtagcgca
     3841 gattgccacg cactttgtaa tgcgggaaca gccgcgcatc ctgaaaaaca tagccaacgc
     3901 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgaccattga
     3961 ggacaatcct ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     4021 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     4081 tagtcaggca atggttgccc aacgtctggg aaaaattcag ttccagcatg attagcgccc
     4141 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     4201 cgcaatagaa ataatgcaca gtctcgctgc gccactttcc ccgccggggg tctgaatcag
     4261 ggtatacatg gcagaaggaa tggttcgcgt ttcaccagga atgttcgaca caaaggtgat
     4321 ggttgcgcca aattcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     4381 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt
     4441 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     4501 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     4561 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgcc ccatcgaaac
     4621 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     4681 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     4741 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     4801 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     4861 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     4921 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     4981 tttacccctt tgctagcaac tgcgtcagaa ccgtaaacaa tgcccaacgg cgcttcattg
     5041 cgttcgacca acgccagcgc gccacgaaca tcttccgccg gagccagttt cggggagagc
     5101 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     5161 tccggatcgc caaccgccag gcgaccgcca ttcagcagtg aagtccagtt ggttttgctg
     5221 tcgatggtaa aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     5281 agcagtgtct gacgcgtagc tgtatcgatc gcttttttat caaccgcata atccatccat
     5341 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     5401 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     5461 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     5521 tctgccagtg cattgccagc aacagcgaaa gagagtgccg ccccagcaaa caagttcaac
     5581 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     5641 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     5701 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     5761 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     5821 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     5881 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     5941 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     6001 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     6061 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     6121 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     6181 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     6241 caggcggtaa aggtggcggc ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     6301 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     6361 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactccaa accagcgccc
     6421 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     6481 atgtcttact ggctgatgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     6541 cgcgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     6601 gcattactca ggacgaagcg gtcgcgcaaa acgctgacaa ccaattaccg ggtattatta
     6661 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccg gacgggcaaa
     6721 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaagga cagaatgtca
     6781 cggcctactt taatgccgac agcgtgatta tcgccacact gtgctaagcg tgttgacaat
     6841 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     6901 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     6961 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa
     7021 tcggccctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     7081 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     7141 tggcagcgga ataacaccga tatgctcggc cctggcgaag atgacaccgg acgcactacg
     7201 gctgagatta ttcaggatga agtaaaggat gcaccgcgct gcatgcaact ggcacagcag
     7261 ttcggtatta cccccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     7321 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     7381 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     7441 cagtccggta ttactctggt actggtgctc aatcgcttcg atgagatccc ggagtttgtc
     7501 cagtttgctg gcgtgctggc ggattgcacg ttagcggaaa ctggcgctaa agaggaactg
     7561 ctccaacaag cactcgtcgc gcaactggcg catagtgaac agcttgaagg tgtgcaactg
     7621 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaacc gcgcattgtg
     7681 ctgaataatg gcgtggtttc ttataacgat cgccccattc ttaataacct tagctggcag
     7741 gtgaatccag gtgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     7801 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga
     7861 cgacgtcgtg gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc
     7921 agtagtttgc atctggatta ccgggtcagt actactgtgc gtaatgtgat tctttctggc
     7981 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     8041 cagtggctgg atattctcgg cattgataaa cgcacggctg acgctccgtt ccatagtctt
     8101 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     8161 cttattctcg acgaaccatt gcaggggctt gatccgctca atcgccagct tattcgccgt
     8221 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccatgct
     8281 gaagatgcgc cggcttgtat tacccatcgt ctggagttcg tgccggacgg tggcctctat
     8341 cattatgcgc tgacaaaaat aaactaagtc ggtaatgctg actgtgccgg atgcggcgta
     8401 aacgctttat ccggcacacg gtcagacgca tccaggcatg aaactgcgtc tttttttcag
     8461 ataaaaagcg caatcattca caatccctct gttttataat cacttaatcg cgcataaaaa
     8521 acggctaaat tcttgtgtaa acgattccac taatttattc catgtcacac ttttcgcatc
     8581 tttgttatgc tatggttatt tcataccata agcctaatgg agcgaattat gagagttctg
     8641 gtcactggtg gtagcggtta cattggaagt catacctgtg tgcaattact gcaaaacggt
     8701 catgatgtca tcattcttga taacctctgt aacagtaagc gcagcgtact gcctgttatc
     8761 gagcgtttag gcggcaaaca tccgacgttt gttgaaggcg atatccgtaa cgaagcgttg
     8821 atgaccgaga tcctgcacga tcacgctatc gacaccgtga tccacttcgc cgggctgaaa
     8881 gccgttggcg aatcggtaca aaaaccgctg gaatattacg acaacaatgt caacggtact
     8941 ctgcgcctga ttagcgccat gcgcgccgct aacgtcaaaa actttatttt tagctcctcc
     9001 gccaccgttt atggcgatca gcccaaaatt ccatacgttg aaagcttccc gaccggcaca
     9061 ccgcaaagcc cttacggcaa aagcaagctg atggtggaac agatcctcac cgatctgcaa
     9121 aaagcccagc cggactggag cattgccctg ctgcgctact tcaacccggt tggcgcacat
     9181 ccgtcgggcg atatgggcga agatccgcaa ggcattccga ataaccttat gccatacatc
     9241 gcccaggttg ctgtaggccg tcgcgactcg ctggcgattt ttggtaacga ttatccgacc
     9301 gaagacggta ctggcgtacg cgattacatc cacgtaatgg atctggcgga cggtcacgtc
     9361 gtggcgatgg aaaaactggc gaacaagcca ggcgtacaca tctacaacct cggtgctggc
     9421 gtaggcagca gcgtgctgga cgtggttaat gccttcagca aagcctgcgg caaaccggtt
     9481 aactatcatt ttgcaccgcg tcgcgagggc gaccttccgg cctactgggc ggacgccagc
     9541 aaagccgacc gtgaactgaa ctggcgcgta acgcgcacac tcgatgaaat ggcgcaggac
     9601 acctggcact ggcagtcacg ccatccacag ggatatcccg attaaggaac gaccatgacg
     9661 caatttaatc ccgttgatca tccacatcgc cgctacaacc agctcaccgg gcaatggatt
     9721 ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg cgcaggaaac gccagccaaa
     9781 caggtgttac ctgcgcacga tccagattgc ttcctctgcg caggtaatgt gcgggtgaca
     9841 ggcgataaaa accccgatta caccgggact tacgttttca ctaatgactt tgcggccttg
     9901 atgtctgaca cgccagatgc gccagaaagc aacgatcctt tgatgcgttg ccagagcgcg
     9961 cgcggtacca gccgggtgat ctgcttttca ccggatcaca gtaaaacgct gccagaactg
    10021 agcgttgcgg cattgacgga aatcgtcaaa acctggcagg agcaaaccgc agaactgggg
    10081 aaaacatacc cgtgggtgca ggtctttgaa aacaaaggtg cggcgatggg ctgctctaac
    10141 ccgcatccgc acggacagat ttgggcaaat agcttcctgc ctaacgaagc tgagcgcgaa
    10201 gaccgcctgc aaaaagaata ttttgccgag cagaaatcgc caatgctggt ggattatgtt
    10261 cagcgcgagc tggcagacgg tagccgtacc gttgtcgaaa ccgaacactg gttagcagtt
    10321 gtgccttact gggctgcctg gccgttcgaa acgctacttc tgcccaaagc ccacgttttg
    10381 cggatcaccg atttgaccga cgcccagcgc agcgatttgg cactggcgtt gaaaaagctg
    10441 accagtcgtt atgacaacct cttccagtgc tccttcccct actctatggg ctggcacggc
    10501 gcaccgttta atggcgaaga gaatcaacac tggcagctgc acgcgcactt ttatccgcct
    10561 ctgttgcgct ccgccaccgt acgtaaattt atggttggtt atgaaatgct ggcagaaacc
    10621 cagcgagacc tgaccgcaga acaggcagca gagcgtttgc gcgcagtcag cgatatccat
    10681 tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa aaacacaatc tctgtttgcc
    10741 aacgcatttg gctaccctgc cactcatacc attcaggcgc ctggccgcgt gaatttgatt
    10801 ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga ttatcaaacc
    10861 gtgatcagct gtgcgccacg cgatgaccgt aaagttcgcg taatggcagc cgattatgaa
    10921 aatcagcttg atgagttttc ccttgatgcg cccattgtcg cgcatgaaaa ctatcaatgg
    10981 gcgaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag cttcggcggt
    11041 gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc ttccgcttca
    11101 ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct ggacggcgca
    11161 caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg ttggctgtaa ctgcgggatc
    11221 atggatcagc taatttccgc actcggcaag aaagatcatg ccttgctgat tgactgtcgc
    11281 tcactgggga ccaaagcagt ttccatgccg aaaggtgtgg ctgtcgtcat catcaacagt
    11341 aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca gtgcgaaacc
    11401 ggtgcgcgtt tcttccagca gccagctctg cgcgatgtca ccattgaaga gttcaacgct
    11461 gttgcacatg agctggaccc aatcgtggcg aaacgcgtgc gtcatatcct gactgaaaac
    11521 gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg tatgggcgag
    11581 ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt gccgcaaatt
    11641 gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt acgcatgacc
    11701 ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta
    11761 cagcaagctg tcgcagaaca atatgaagca aaaacaggta ttaaagagac tttttacgtt
    11821 tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg gcacccgatg
    11881 gtcagccgta ccgactgtta actttgcgta acaacgcagg gatggtagtc acgctgatgg
    11941 actggggtgc gactttactt tcagcccgta ttccgctttc cgatggcagc gtccgcgagg
    12001 cgctgctcgg ctgtgccagc ccggaatgct atcaggatca ggccgcgttt ctgggtgcct
    12061 ctattggtcg ttatgccaac cgcatcgcca atagtcgtta tacctttgac ggtgaaaccg
    12121 tgacgctttc gccaagtcag ggcgttaacc agctgcacgg cggcccggaa gggttcgaca
    12181 aacgtcgctg gcagattgtg aaccagaacg atcgtcaggt gctgtttgcc ctgagttcag
    12241 atgatggcga tcagggcttc ccgggtaatc tcggcgcgac ggtgcaatat cgtctgaccg
    12301 acgataaccg tatctccatt acttatcgcg ccacagttga taaaccttgc ccggtgaata
    12361 tgactaatca cgtctatttc aatcttgacg gcaagcagtc tgacgtgcgc aatcacaagt
    12421 tgcagattct ggcggacgaa tatctgccgg ttgatgaagg cggtattccg cacgacggcc
    12481 tgaaatctgt cgccggaacg tcttttgatt tccgcagcgc caaaatcatc gccagtgagt
    12541 ttcttgccga cgacgatcag cgcaaagtga aaggttacga tcacgcattc ttgttacagg
    12601 ccaaaggcga tggcaagaaa gtggcggcgc atgtctggtc agcagatgaa aaattgcagc
    12661 tgaaggtcta caccaccgct ccggctctgc aattctactc cggcaactac ctcggcggca
    12721 caccgtcgcg gggaaccgaa ccttacgccg actggcaagg gctggcgttg gaaagcgagt
    12781 ttctgccgga cagcccgaac caccctgaat ggccgcaacc ggactgtttc ctgcatcctg
    12841 gtgaagagta ttccagcctg acggaatatc agtttattgc tgagtaatgt taagccctcg
    12901 tcatgagggc tttatctcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    12961 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    13021 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    13081 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    13141 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    13201 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacacct
    13261 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    13321 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    13381 tgaacaaagc ggaaactgct gaaaaatatg gcgacgagca ggtgaaacag tggcgtcgtg
    13441 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    13501 cgcgttacgc gaaactgagc gagaaagaac tgccgctgac ggaaagcctg gcgctgacca
    13561 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    13621 gcgtaatcat cgctgcacac ggtaactcgt tacgtgcgct ggtgaaatat ctcgataaca
    13681 tgagcgaaga agagattctt gagctgaata tcccgaccgg cgtgccgctg gtttatgagt
    13741 ttgacgagaa cttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    13801 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaat
    13861 gagaaagccg actgcaagtg agtcggcttt tttgtttgtc gtcgctaaca atggagcaca
    13921 ttgcctgatg cgacgctgcg cgtcttgtca gggctacggt gattcatcgg atacgccact
    13981 ctgacggcgc atccgacaat taaactttac ccgcgacgcg ctttcactgc gttcgccagt
    14041 tgacgtaaca gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttacca
    14101 taggccagcg gctccccgct ctccaggctc tgattgcctt ccaccagatg actttccacc
    14161 atcacaccga taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    14221 tccatctgct ttttgaattg tttggacgaa ttagcatggc tgaaatcgat catcacctga
    14281 gctggcagac ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    14341 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccgct ggtattcaca
    14401 atcgccgaat gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta
    14461 atggcatcga tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa
    14521 gaaagaccag acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg
    14581 ccccagctca tcaggtcagc gagatactga ggagtgatca tatcgaggaa ttcacccgcc
    14641 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    14701 ttgatctgga agctgttatc catatgcgga tcgttaatca gccctttcca gcccaccgta
    14761 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    14821 cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac
    14881 gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    14941 cgggcatggg caaccgtatt cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg
    15001 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    15061 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    15121 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    15181 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    15241 gtttgttaat tatttcaaga ttctctgctg gatttctttg cctggctgaa atcttaaacc
    15301 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    15361 aacaggagca tcctatgaaa atgacaaaac tggccacact atttctgact gccactctaa
    15421 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaaccaat aacggccagg
    15481 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    15541 ataacgtcga caataacggg gtaaataccg gttctggcgg cactatgctg catccggatg
    15601 gttcttcaat gaacaatgac ggcatgacca aagatgaaga gcacaagaac accatgtgca
    15661 aagatggtcg ctgcccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    15721 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    15781 gagcggagtc tctccctttt aatttttccc ttctaaaagc agtatgatgt cataaagtta
    15841 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    15901 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    15961 tatgctggta gaagtcattg gtggttttct ttccggttct ctggctttgc tggccgatgc
    16021 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    16081 acgtcgtcct cccaccattc gccacacttt cggttggcta agattgacca cgctcgcggc
    16141 gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga
    16201 acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg atggcaattg ccgtggctgg
    16261 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    16321 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    16381 tattgccgcg ttgattatta tctggaccgg ctggacacct gctgacccca ttctctcgat
    16441 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    16501 attacttgag ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    16561 ggaaatcccg gaagtccgca atgtgcacca tgtgcatgta tggatggtag gcgagaagcc
    16621 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    16681 gatccaacac tacctgatgg atcactatca gattgagcac gccaccattc agatggaata
    16741 tcaaccttgt catgggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    16801 tcaccattaa tgggacagcg cgcgtgagcc tctttcgcgt gcgctattga tccacatccg
    16861 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    16921 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    16981 cacatactta cgagtcatca gaatcattgc cacgatagat aacaccatca tgcaagaatc
    17041 ccagaacggg aaagcgtccg gttgtagttc aggcatcgca acctgtaatc ctaatgcttg
    17101 catgatgatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    17161 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    17221 gcgaattttt aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta
    17281 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    17341 gaaaataatg ccaaacaagg tgacgttgat caggccaaag aagtagttgc tgatcttctc
    17401 cagactggca aggccaatac acagcaaccc ggcgattgtg cctaccgctt cgatccatga
    17461 gagatcataa ccgcctgctc ctattggtat atgtaccagg atattctgca cactaaaaaa
    17521 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    17581 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctttcatcaa
    17641 catgaacctc gtaattgctt ccttcctgtt ctaatgcctc agcgatggcc tggaggccat
    17701 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg
    17761 gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat
    17821 cggttgccac aataagcctc tgatgtggca atgttttcgc agcagcgatc agttgactgg
    17881 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    17941 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aactcatcat
    18001 gcacaatgca ggcaccctgc cagcataaaa tgtctgcgcc cgtctttttt tgcacgtaac
    18061 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatcc agatgatcaa
    18121 taagttcgac ggcaatgctt gaagttacca cccaatctgc ccgcgctttt accgcagcag
    18181 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgcaaaat gcgttaaatt
    18241 cttcaacagg gcagccgaga tccagtgaac actcagcctg aagtgtcggc atcagaattg
    18301 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    18361 acgtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    18421 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    18481 ccgcattacg ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    18541 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatca
    18601 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtgatatt
    18661 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    18721 gtcactcgaa ttacgtatga gatgccgaat gccacacttt gcgtcttatt cctccgacgt
    18781 aaagttgttt acgcagcaag cgcggagtat gtcatcaggg tcaagaattg aaattgagag
    18841 atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg
    18901 attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc
    18961 atacgggtgt tacctgaaag atttctactc ggaaccaatc tttaaaattg gtgggtcgtg
    19021 caggatgact cgcttcgctt gccctacggg ccgttgctga cgcaacgtta tccttcacgc
    19081 ttaacatctg agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa
    19141 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg
    19201 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
    19261 cgatactgcg ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt
    19321 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
    19381 aacgacccgg tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    19441 tgctctccca gctgagctaa tcacccgata ttacgctgga tactacaact aactaggtta
    19501 gtggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
    19561 aactgagcta acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg
    19621 gcggcatata ttactgattt cagacttgag cgcaacaaaa atttcgatgt agatcactca
    19681 actgcttatg attcgcacga cacgaccaga aataatgcga cttctggtcg tgtgttatgc
    19741 attacatcgc gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac
    19801 tgataacctg ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga
    19861 cgccgacttt aaacatcgca tctgcagcct ttggtgactt cggatagttt ttcactaccg
    19921 aagcaaaata gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc
    19981 aataattggc gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg
    20041 ccaccattgc gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat
    20101 ccgtgtttgc atcaccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg
    20161 tcggcgtcgt tgatgccgcc gcaccgcttt gatcaccgct ggttgattgc gccgctgcac
    20221 caccgctgct gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat
    20281 tcagttgata ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat
    20341 cagagagttg ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa
    20401 gttgaatgac gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa
    20461 aagcggccca gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga
    20521 agttactgct catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg
    20581 ccgcttcgtc atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct
    20641 ggtctgcaga aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac
    20701 ccagggagat gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt
    20761 tgtaagacgg gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag
    20821 aacggatatc gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca
    20881 tttgcagacg agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac
    20941 cagtgccggc acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac
    21001 atgccgcaat tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt
    21061 gcatttcaat gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca
    21121 gggaatttga cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa
    21181 accaaattca gcacggatcc catcccctga gaagagctgt agattaccat agtgccgtta
    21241 ggtgccagac ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt
    21301 gccagatctt gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat
    21361 ttaccgtcgc tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt
    21421 ggcgcaccgc cgttgatatt cactttataa acctgtggac gaccggcctg gtcagaagta
    21481 aatgccaggt tctggctgtc cgggaaccag gtcggttcgg tattgttact gcgaccatca
    21541 gtcacctggc ggatctgacc agaagccaaa tccattacgt acaggttcag gctaccggtt
    21601 ttcgacaagg cgaatgccag tttgctgccg tctggcgaga atgcaggcgc accgttgtga
    21661 cgcgggaatg aagccacctg acgtacagcg ccgtttgcca gcgtttgtat aaccagcgcg
    21721 gaacgaccgc tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccggg
    21781 gacatcagcg gctgtggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat
    21841 acgcgcagtt catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta
    21901 cggaacgcac ctttaatacc ggtcagtttt tcaaacactt catcactggc ggtatgacca
    21961 gcataacgca gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt
    22021 gcgccgccag tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg
    22081 acctgaccga caactacaac gtcaataccc agcgcggacc atgcagctgg ttgtacttcc
    22141 tgcgcactac ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg
    22201 ttacgcaagt cggcagcaac gatgccgcca atatcttcag gtgccgcacc agggcccgcc
    22261 cactggaaag gaacaactcc aataggacga ccggaatcta caccgctgtc gatcacaatg
    22321 cggacttcag catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat
    22381 gcctgcttca tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt
    22441 taacaaacta aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac
    22501 cgtcggaaca gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact
    22561 tcatatactg cttggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca
    22621 gcctgacaaa gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt
    22681 gccagtttta tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta
    22741 ctttcgatag cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca
    22801 ttgtttttag tattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc
    22861 gttttcggtg cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctccgcggca
    22921 gcttttgctg cagcggcttt ttcagctgct gcttttgcag ctgccgcttt tttgtctgcg
    22981 gcggcttttt ctgccgctgc tttcttatca gctgctgcct tttcagcagc cgctttttct
    23041 gctgcttttt tgtcggctgc agctttctct gctgcttttt tgtcggctgc agctttctct
    23101 gctgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    23161 agcgccgcag ctgctgcctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    23221 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg ctgcttcttc cgcagcttta
    23281 tcatccgctt cggccttcgc tttagcatct gccgccgctt tcgctgccgc ctcttccgct
    23341 tgcttctgct ttaactcggc ctgttttgcg gcttcttcag cctgcttttt ctgttcctga
    23401 gcagctaacc gctctttctc aagttgcttc aggcgttcct gttcagccgc ttgtttctca
    23461 cgcagttctt cagcagcctg ctgttccttc atcttgcgct gctcatcaga acgcttcgcg
    23521 cttgattcct ggctttgcat gcgtttgtac tgctcaacta ccgcacctga atcaaccatg
    23581 acagcgtcga tggacgaacc accgccgcct ccagctgaag cttctatatt ctcatcgaac
    23641 gaactccaga tcagcgccgc aaataagatg acatgcagca ctgctgaaat aattatcgcc
    23701 cgcttgagct tgtcgttttg ttcggttgcc tttgacactc tcggtttcca aaaactgttc
    23761 gcctgttacc cgctctcttt caagcaaggg aaacgcagat gtttagatag gctgcgtcat
    23821 taaaccaacc gatttcacac ccgcactatg taacaagttc agtgctttaa ttatttcatc
    23881 gtaaggcaca tcttttgcgc caccgatcag aaagaccgtt ttcgggttgg ccttgaaacg
    23941 gctggacact tccgccacca cctgctctgg tggtaaacgc tccaggcgat ctttctcaac
    24001 caccacggtg tactgaccaa taccagacac ttcaacaatc actggcggat tatcgttact
    24061 gctcaccgcc tgtgattcag tagcgtctgg caaatcgacc tccacgctct gggtgatgat
    24121 gggcgctgtc gccataaaga tcagcaacag caccagcagt acgtccagca acggtacaat
    24181 gttgatttcg gacttgagat cgcgacgacc tcgtccacgc gctctggcca tggcttaccc
    24241 cttgttgctc tcgctaacgg taaacgcctg gcggtgcaga atcgcggtaa actcttccat
    24301 aaagttgtcg taattcagtt ccagtttgtt tacacgttgg ttgagacggt tataagccat
    24361 taccgccgga atagcggcga acagaccaat tgccgtcgca atcaacgctt ctgcgatacc
    24421 gggcgcaacc atttgcagtg ttgcttgttt taccgccccg agggcgataa aggcgtgcat
    24481 gatcccccag accgtaccaa acagaccaat atacgggctg atggagccaa ccgtaccgag
    24541 gaacggaatg tgcgtttcca gattttcaag ttcgcggttc atggaaatac gcatcgcacg
    24601 cgacgcccct tccactacgg cttccggcgc atggctattg gcacgatgca ggcgcacaaa
    24661 ttctttgaac ccgctgtaaa agatttgttc cgaaccagtc agattatccc gtttcccctg
    24721 gctctcttga tagaggcgag agagttcgat tccagaccag aatttatctt caaacgcttc
    24781 ggcttcgcgc gccgctgcgt taagaatacg ggtccgctgg ataataatgg cccaggatgc
    24841 gattgaaaaa ccaatcaaaa tcaacatgat aagtttaacc agaaggctag ccttcaggaa
    24901 caaatcaagg atattcatgt cagtcactgc ttaaactccg cgacaataga cttgggaagc
    24961 gcacgaggct tcattttgag tgggtcaacg caaacaacca gaacctctgc ttcattcagc
    25021 aaagtattct cggcgttgac aatacgttgc gtgaaaacca aagaggtgcc acgcattgat
    25081 gttatttcag tctgtatttc gagcatatcg tcgagccgcg caggtgcgta atattccacc
    25141 gtcattttac gtaccacaaa ggcaacgcgt tcagccatca gcgcctgttg actgaagtga
    25201 tgatgacgca gcatctctgt gcgtgctctt tcataaaaag cgacgtaact ggcgtggtac
    25261 accacaccac cggcatcggt atcttcatag tagacgcgaa ccggccatcg aaacagcgtt
    25321 gtattcactt tacatcccgg taatgcaaca aaagttagag cttttaaacg ctgctactat
    25381 acgcgcgcga ttgaggtttg ggaatgaggg caagttaagg gagcgtaaaa atttatgggc
    25441 ctggcacggc ccataatttt ttaaagagaa ttctgattta aaagaagaag aaaatcagcc
    25501 cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa agaaccttct
    25561 gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc agcaataaac
    25621 catgccagat ttccagttca ctggtcttcg cggcgaaacg agatgggtcc caaaacatac
    25681 atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc ttgtccatta
    25741 ccgcatacaa ggttgcgata atcttactca tcagatgtct tcttgaccgg ctttgcctga
    25801 ttcgacgtgc tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa gaagcgttcc
    25861 cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga gtgggtgtta
    25921 cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca ggcggtgtag
    25981 agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag tgtcagctgg
    26041 ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga cggcatcaca
    26101 aacgggaaca ttgcgatacc ggcagtcagg atgatgcagg ccagcgtcag ggaggagaac
    26161 acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag cggcagaacc
    26221 acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac cagccatgcg
    26281 ccagcttcac gaaccacttc tttattcagc gggttagagg ctgcgtaatg gtccattgtc
    26341 gatttcacga cataaccatc gataccgtac atcacccata cgccagccag tgcgaaacag
    26401 accagtgtca ccagtgccgc cacctgagcc gttgcacggg tacgcaggtg cagttcgccc
    26461 acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac gctcaccacg
    26521 cctgccagca ggccgaacgg gttaagcagc tggaagaagt taccggtgta gtacagacgc
    26581 agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc tacaccaatc
    26641 accaacggcg gaacgaagct accaatgaag atgccccagt cccacatgtt acgccagcgg
    26701 gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa agacgccagc
    26761 acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat cggccaggca
    26821 gcaaacagtg cgccgcctgc ggtgatcagc caaacctggt taccgtccca gtgtggtgcg
    26881 atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg ggtgagcatg
    26941 cccaccccca tgtcgaaacc atcagtgact gcaaaaccaa tcagcagaac gccaaccagc
    27001 agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct gtcttagcgt
    27061 gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt caggctgctt
    27121 gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa cagggtatac
    27181 aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga cgagttcgcc
    27241 acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc agccacgaac
    27301 cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc gcgcaggagc
    27361 cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc gatgattgcc
    27421 agcagcagga agccacacgc caccatgata cggaacgcga agtacagcgg cgctacacgc
    27481 ggaatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc cacgtttggc
    27541 gtatagcgtt tcagcagcag accgtaaccg aggtccttct tcatgctatt gaactggtca
    27601 cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta cgccttcatc
    27661 ccgttacgaa tgcgttcttc atgctgcacc atcagttctt tcaggccgat aactggagta
    27721 tccacggaac gcgttgcaat tatgcccagc gcgtaaggga tctggatcgc aaatttgttc
    27781 gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc aggttgcgtt
    27841 tcccactcag cttcaatagc agccagtttg gttttctgca cgtcgcccat ttcgtagccg
    27901 gattcatcac ccagaacaat aacagacaga acggcagcca taccgaagct ggcagcgata
    27961 gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca tgcgctgata
    28021 ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac gaatttcacc
    28081 tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg catagtttca
    28141 aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag aatccacagt
    28201 gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg aactttaccc
    28261 agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa gaaggccatc
    28321 agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga atagtaagac
    28381 cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag agcgaagttg
    28441 ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt gccggaaagg
    28501 acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag tggcacaaaa
    28561 aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac tatatctaac
    28621 atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta gaaagggtca
    28681 cacgcatgcc ccaatacaaa attatttgct cgcctctatc gttgccattt catcaaaaga
    28741 ggaaaagaca atgataaaaa agtta
//
LOCUS       DAZGXS010000051        28265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGXS010000051 DAZGXS010000000
VERSION     DAZGXS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHKG5_002344"
     CDS             complement(<1..209)
                     /locus_tag="ACHKG5_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0868440.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKG5_002345"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKG5_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0868441.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKG5_002346"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKG5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0868442.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKG5_002347"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKG5_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0868443.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKG5_002348"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKG5_002348"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0868444.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAVVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKG5_002349"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKG5_002349"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0868445.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKG5_002350"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKG5_002350"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0868446.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKG5_002351"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKG5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0868447.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKG5_002352"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKG5_002352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0868448.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKG5_002353"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKG5_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0868449.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKG5_002354"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKG5_002354"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0868450.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKG5_002355"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKG5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0868451.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKG5_002356"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKG5_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP0868452.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKG5_002357"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKG5_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP0868453.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKG5_002358"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKG5_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0868454.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKG5_002359"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKG5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0868455.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKG5_002360"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKG5_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0868456.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKG5_002361"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKG5_002361"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0868457.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKG5_002362"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKG5_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0868458.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKG5_002363"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKG5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0868459.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKG5_002364"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKG5_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0868460.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKG5_002365"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKG5_002365"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0868461.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKG5_002366"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKG5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0868462.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKG5_002367"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKG5_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0868463.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKG5_002368"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKG5_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0868464.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKG5_002369"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKG5_002369"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0868465.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKG5_002370"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKG5_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0868466.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKG5_002371"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKG5_002371"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0868467.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKG5_002372"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKG5_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0868468.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKG5_002373"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKG5_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0868469.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKG5_002374"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKG5_002374"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0868470.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6833 a         7592 c         7135 g         6705 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactacggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttct
//
LOCUS       DAZGXS010000052        28261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGXS010000052 DAZGXS010000000
VERSION     DAZGXS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(101..1498)
                     /gene="ccp"
                     /locus_tag="ACHKG5_002375"
     CDS             complement(101..1498)
                     /gene="ccp"
                     /locus_tag="ACHKG5_002375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0868471.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1903..3552
                     /locus_tag="ACHKG5_002376"
     CDS             1903..3552
                     /locus_tag="ACHKG5_002376"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0868472.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3603..4205)
                     /locus_tag="ACHKG5_002377"
     CDS             complement(3603..4205)
                     /locus_tag="ACHKG5_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP0868473.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4725..5624
                     /locus_tag="ACHKG5_002378"
     CDS             4725..5624
                     /locus_tag="ACHKG5_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0868474.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5673..6686
                     /gene="yhjD"
                     /locus_tag="ACHKG5_002379"
     CDS             5673..6686
                     /gene="yhjD"
                     /locus_tag="ACHKG5_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0868475.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7098..8420
                     /locus_tag="ACHKG5_002380"
     CDS             7098..8420
                     /locus_tag="ACHKG5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0868476.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8602..10662)
                     /locus_tag="ACHKG5_002381"
     CDS             complement(8602..10662)
                     /locus_tag="ACHKG5_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0868477.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10732..11499)
                     /gene="pdeH"
                     /locus_tag="ACHKG5_002382"
     CDS             complement(10732..11499)
                     /gene="pdeH"
                     /locus_tag="ACHKG5_002382"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0868478.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11731..12660
                     /gene="kdgK"
                     /locus_tag="ACHKG5_002383"
     CDS             11731..12660
                     /gene="kdgK"
                     /locus_tag="ACHKG5_002383"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0868479.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12756..14252)
                     /locus_tag="ACHKG5_002384"
     CDS             complement(12756..14252)
                     /locus_tag="ACHKG5_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0868480.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14473..15759)
                     /gene="dctA"
                     /locus_tag="ACHKG5_002385"
     CDS             complement(14473..15759)
                     /gene="dctA"
                     /locus_tag="ACHKG5_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0868481.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15942..17930)
                     /gene="hmsP"
                     /locus_tag="ACHKG5_002386"
     CDS             complement(15942..17930)
                     /gene="hmsP"
                     /locus_tag="ACHKG5_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0868482.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18012..21485)
                     /gene="bcsC"
                     /locus_tag="ACHKG5_002387"
     CDS             complement(18012..21485)
                     /gene="bcsC"
                     /locus_tag="ACHKG5_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0868483.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHCDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21467..22573)
                     /gene="bcsZ"
                     /locus_tag="ACHKG5_002388"
     CDS             complement(21467..22573)
                     /gene="bcsZ"
                     /locus_tag="ACHKG5_002388"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0868484.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22580..24928)
                     /gene="bcsB"
                     /locus_tag="ACHKG5_002389"
     CDS             complement(22580..24928)
                     /gene="bcsB"
                     /locus_tag="ACHKG5_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP0868485.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24939..27557)
                     /gene="bcsA"
                     /locus_tag="ACHKG5_002390"
     CDS             complement(24939..27557)
                     /gene="bcsA"
                     /locus_tag="ACHKG5_002390"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP0868486.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27554..28261)
                     /gene="bcsQ"
                     /locus_tag="ACHKG5_002391"
                     /pseudo
     CDS             complement(27554..28261)
                     /gene="bcsQ"
                     /locus_tag="ACHKG5_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6669 a         7821 c         7117 g         6654 t
ORIGIN      
        1 atcttttata atataaacat ttaatatgat aatattaaaa acagacttta ttatcgtaat
       61 aaaaatcgcc caaacattcc tgttcgggcg atcaaattaa ttattgttta tcctgcatat
      121 acggcgtgta aaccccgttc agactgtgca ggaaagctac gatatcatcc acatcctcct
      181 gcggcagctc tttgcctacc tgatagcgca acatcagttt caccgccccg tccagcgtcg
      241 gcacgtcacc gcgatggaag tacggtgccg ttaaagcaac gttacgtaaa ccgggtactt
      301 tctgacgcaa tttatcacgc tcttctttag tcacattcat acgaccaata tccgccgccg
      361 taatttcccc aaagttaaag tcttttttca gccccaacgg ttcgaaggaa cgtccgccga
      421 gaataattcc accatgacaa gttgcacatt tattatcttt aaataattga tagccttttt
      481 tctgttgcgc cgtcagcgca ttttcatccc cacgcaacca tttatcaaat ggggaatccg
      541 gcgtaattaa tgttttctca aattcagcaa tggcatcagt aatattttcg ccactgaaac
      601 cttgcggata gacgtcgagg aactgcgctt taagctgcgg atctttttcc agcttagcaa
      661 taatttcgtc ccaggatttt gacgccattt caatcgggtt caacggcggt ccaccagcct
      721 gatcctgcaa tgttgccgca cgaccatccc agaactgctc aacgttaaat actgagttaa
      781 ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac accaatcgat gtttttctgc
      841 catcgacgcc ccccgcattc aacgcatggc aatgagcgca tgaaatggtg ctatcagccg
      901 ataaacgggg atcgtgatac agcgcaaagc ccaacgccac tttttgcgca tcggtaggca
      961 gtttttgcgg gatgggctgc accggttcat tgcgatgctc cggagcagta tcattgctgg
     1021 cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat ttccgcccgc tcttcatcac
     1081 tcaccttacc cgcccagtgt agcgcggtat aacgcgttgg cggcatagtt tcatactgca
     1141 tcacccattc aatcttattc agatcgcttt gcgaaacggg tttatcagcc agcagagcag
     1201 cacgcacggc ctcgaggtta aaagatttat atccaagctt aatgtcgtaa tccatcaact
     1261 gtttcgcgcc aggaatataa taataggcgg gtaattctgc cgaaggcgtg tggcaatagt
     1321 cgcatccttt ttcgcggaga aagcctaaaa ccttattatt ttcactgaca gcagatgcct
     1381 gaacatcggc ctgtttactg cgtttattat cgtggtacca cacataacca gataacccta
     1441 aatagcaaat cgcgacgcca gccaggccga tcgcggtaat acgtgagacc attttcatta
     1501 ttcttccctc acggtggtga gttattgtga cccagaaaat tttccagaat gctgcaaacg
     1561 tctctgcgag acaaagccat cataaaaaac agggagatac gctttttgat aggaatcaat
     1621 caattacatg aatataaact atcaataaga tagcctgtgc ctattaccca gatcaggaat
     1681 ggcatatcat gattgcggca agagcaggat tgaatgttct gaaaatgaaa aacaacacga
     1741 aatctgtatt tttataaatt ccattgatat tagtgcgtta tattttttga caggttaata
     1801 aacgtgatcc accgcacgct ttgtcgccca ccaggcggag cgaatgacta cccttaaaga
     1861 aaagcccgat aattagcgac gaatttcgga ggttggatcc ttatgctcaa tcagaaaatt
     1921 caaaacccta atccagacga actgatgatc gaggtcgatc tctgctatga actggacccg
     1981 tatgaattaa aactggatga gatgatcgag gcagaaccgg aacccgagat gattgaaggg
     2041 ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc tcgaactgtt cgagcatgtt
     2101 cagtcggcga aaattttccc cgacagtaaa acctttcccg actgcgcacc caaaatggac
     2161 ccgctggata ttttaatccg ctaccgtaaa gtgcgccgtc atcgtgattt tgacttgcgc
     2221 aagtttgttg aaaatcactt ctggctgccg gaggtctact ccagcgagta tgtatcggac
     2281 ccgcaaaatt ccctgaaaga gcatatcgac cagctgtggc cggtgctaac ccgcgaacca
     2341 caggatcaca ttccgtggtc ttctctgctg gcgctgccgc agtcatatat tgtcccgggc
     2401 ggccgtttta gcgaaaccta ctattgggat tcctatttca ccatgctggg gctggcggaa
     2461 agtggtcggg aagatttgct gaaatgcatg gccgataact tcgcctggat gatcgaaaac
     2521 tacggtcaca tccccaacgg caaccgcacc tattatttga gccgctcgca accaccggtt
     2581 tttgcgctga tggtggagtt gtttgaagaa gatggtgtac gcggtgcgcg ccgctatctc
     2641 gaccacctga aaatggaata tgccttctgg atggacggtg cagaatcgtt gatccctaat
     2701 caggcctatc gccatgttgt gcggatgccg gacggatcgc tgctcaaccg ttactgggac
     2761 gatcgcgaca cgccgcgtga cgaatcctgg cttgaggacg ttgaaaccgc gaaacattct
     2821 ggtcgcccgc ccaacgaggt gtaccgcgat ttacgcgcgg gggcggcctc cggttgggat
     2881 tactcttccc gttggctccg tgatactggt cgtctggcga gcattcgtac cacccagttc
     2941 atccccatcg atctgaatgc cttcctgttt aaactggaga gcgccatcgc caacatctcg
     3001 gcgctgaaag gtgagaaaga gacagaagca ctgttccgcc agaaggccag tgcccgtcgc
     3061 gatgcggtaa accgttacct ctgggatgat gaaaacggca tctaccgcga ttacgactgg
     3121 cgacgcgaac aactggcgct gttttccgct gccgccattg tgccgctcta tgtcggtatg
     3181 gcgaaccatg aacaggccga tcgtctggca aacgccgtgc gcagtcggtt actgacacct
     3241 ggcgggattc tggcaagcga gtacgaaacc ggtgaacagt gggataaacc caacggctgg
     3301 gcaccgttac aatggatggc aattcaggga tttaaaatgt atggcgatga ccttctgggc
     3361 gatgaaatcg cgcgcagctg gctgaagacg gtgaatcagt tttatctgga acagcacaaa
     3421 ctgatcgaaa aataccatat tgccgatggt gttccccgcg aaggcggcgg tggcgagtat
     3481 ccgttgcagg atgggtttgg ctggactaac ggtgtggtac gccgtttaat tggtttgtac
     3541 ggcgaaccat aatattttta cagccagccg ctaacttcct gctggctgta aaattatcct
     3601 cttcaggagg agatatttaa catcattgcc gcctgggtgc gatttttcac ttccagacgg
     3661 cgatacaggg attccagatg cgcttttacc gttccggtac tgatattcag cgctctgccg
     3721 atctccttat ttgattcgcc cgccgctaac atggttaaaa tctcccgctg gcgggcgctt
     3781 aacgatttga gatctttaat gtccttttcc ggcgtcgtcc gccagtctcc aggcagaaac
     3841 attatcccca tcgccgcact atttaccgcc aacgcaaatg tctcgacggt tgaatcacga
     3901 ggcacaatgg ccagcacatt aaaatggata acttcctgta accaccgttt attgcagtcc
     3961 gtcgccgtaa ttaacacctt aacctcagga aattgcacca cggttttttg cagcaaccag
     4021 tagcaaaact caccatcctg atcgccatcg agcataacta aggcttcagg gtaactttcc
     4081 agcttttgcc ataactcgtc tgcctgactg gccccctgaa tactcactcc tggaatacgc
     4141 tgctgtaaac tgattttcat tccatgaata aatattgact gcctgtcaaa catgactatt
     4201 tgcataactg aatctccacc tgaatacgtt aaaaagactt aagtagtgga agggtattac
     4261 ccgcgagaaa aaataagaat tcgccatttg gcggtggcca ttctacagag atggcgcgta
     4321 gaaaatagtt accgatataa atagttacag ctaaacgcct gaaattacat gtcgagggca
     4381 ctatttaaaa caattttgag gatttcctta tattggtggt tagtacgcat gcaattaaaa
     4441 atgaaattcc gcgaccacaa gccaaaataa caaatggcaa tgagacaaaa ataagcacaa
     4501 atagccaata tgtcctctat tcactttaaa gggaatcgct gaaaaatacg ctctgtttaa
     4561 ggggattcac ctttctcaga aagctattcc gcccttttcc tgctgagaaa tcgccacatt
     4621 cgtcatgaca acattgtgaa acccggcatt agatgttaga aaaaataata aacaatgcga
     4681 tatgcgcgtt actgtttcgc tgtgaacaac aatcggtcaa agaaatggat aaaattcacg
     4741 caatgcagtt gttcatcaaa gtcgcggagc tggaaagttt ttcccgcgca gcggatttct
     4801 ttgctttgcc aaaaggaagt gtttcgcgcc agatacaggc actggaacat caacttggca
     4861 cccagcttct ccagcgcacc acgcgacggg tcaaactcac gccagaaggc atgacctatt
     4921 atcaacgagc aaaagatgtg ttgagtaatc tcaacgaact ggacggtctg tttcagcagg
     4981 atgccaccag tatcagcggt aaattacgca tcgacatccc gccaggaatc gcaaaaagcc
     5041 tgttactgcc gcacctgtcg gaatttctct atcagcatcc gggaattgag ctggaactga
     5101 gtagccatga ccgtccggta gatattcttc atgatggttt tgattgcgtg atacgcactg
     5161 gcgcgttacc ggaagatggc gttatcgccc gtcccctcgg caaactgacc atggtcaact
     5221 gtgccagtcc gcactatctg acgcgctttg gttatccgca aaaccccgac gatctcactt
     5281 cacacgcaat agtgcgttat acaccgcacc tgggtgtaca tccgttaggt tttgaggttg
     5341 ccagcgttaa tggcgtccag tggtttaagt ctggcggcat gttgacggta aacagtagcg
     5401 aaaactatct cgccgccggt cttgccggtc tggggattat tcagatcccg cgcattgccg
     5461 tgcgcgaagc cctgcgtgcc gggcggctta ttgaagtgtt gccaggctac cgtgccgagc
     5521 cgctctccct ttcgctggtt tatccgcagc gtcgggagct ttcccggcgt gtaaacctgt
     5581 ttatgcagtg gctggctggc gtaatgaaag agtacctgga ctgaccgact atacttttta
     5641 agaatgacca cgacagaagg acaaaagagc ggatgacgca ggaaaacgag atcaaacgtc
     5701 ccacccagga tctggagcac gagccgatta agcagttgga taatagcgag aaaggcggca
     5761 aagttagcca ggcgctggaa accgtcacca ccactgccga aaaagtccaa cgccaaccgg
     5821 ttattgcgca cctgattcgc gcgacagaac gctttaacga tcggctgggt aaccagtttg
     5881 gtgcggctat cacctatttc tcatttttgt cgatgatccc gattttgatg gtgtcgtttg
     5941 ccgcaggggg ctttgtgctg gcctcccatc cgatgttgct acaggatatc ttcgacaaaa
     6001 ttctgcaaaa catcagcgat ccgacgctag ccgctacgtt gaaaaacacc atcaacaccg
     6061 ccgttcagca gcgtacgact gtagggcttg tcggcctggc ggtggcgctt tattccggca
     6121 tcaactggat gggtaacctg cgtgaagcga ttcgtgccca gtcgcgcgat gtctgggaac
     6181 gctcgccgca agatcaggag aagttctggg taaaatatct gcgtgatttt atttcgctga
     6241 ttggtttgtt gattgcgctg attgtgacgc tttcgatcac ctcggttgcc ggttcggcgc
     6301 agcaaatgat tattagcgcc ctgcacctga acagtattga gtggctgaaa ccgacgtggc
     6361 ggttgattgg cctggcgatt tccatcttcg ccaactatct gcttttcttc tggatcttct
     6421 ggcggttgcc gcgccaccgt ccgcgcaaaa aagccctgat tcgcggaaca tttctggctg
     6481 ctattggttt tgaagtgatt aaaatcgtga tgacctacac cctgccatcg ttgatgaaat
     6541 ccccctctgg cgcagcattt ggttccgtgc tggggctgat ggcatttttc tacttcttcg
     6601 cccgtttgac gctgttttgc gcggcgtgga ttgccaccgc cgaatataaa gacgacccgc
     6661 gaatgccggg gaaaacgcag ccttaaaata ggccggatgc agttcaaaac gccttatccg
     6721 gcatacgaat ttattcggcc tggctccccg taggccgaat aagatgcgcc agcatcgcat
     6781 ccggctataa tgcgcacata acctcttgaa actctttccc agagcctctt cagccatcta
     6841 ttcaggagca aacaatttca tgccaactca taaccccagc atataaatcc agttggtaac
     6901 ttttatttaa cctgaaacca gttttatcca ctatttataa aattatgtga agcatttcat
     6961 agaagaaaaa tcactggcct aaacattatc ccctttttgc ctggtttttg accatttccg
     7021 cgatttgtta cacattgaaa tatcactttt gctgtgcgta atatggctat tcgttagcca
     7081 aaaaataaga aaagattatg caagcaacag ccacaacact cgaccacgag caagaataca
     7141 cgccgatcaa ctcgcgtaat aaagtccttg tcgcctctct cattggcacg gccattgagt
     7201 tcttcgactt ttacatttac gccactgcgg ccgttattgt gtttccgcat atcttcttcc
     7261 cgcagggcga tcctacggca gcaacgctac agtcgctcgc caccttcgcc atcgccttcg
     7321 tcgcgcgccc cattggctct gccgtttttg gtcattttgg cgatcgcgtt gggcgtaaag
     7381 cgacgctggt cgcctcgttg ctaacgatgg ggatttcgac cgtggtgatt ggtctgctgc
     7441 caggctatgc cacgattggt attttcgccc cgctgctgct ggcgctggct cgatttggtc
     7501 agggtctggg cttaggtggt gaatggggcg gcgcggcgct gctggcgact gaaaacgccc
     7561 caccgcgcaa acgtgcattg tacggctcct tcccgcagct gggcgcaccg attggcttct
     7621 tctttgccaa cggcactttc ttgctgcttt cctggctact gaccgacgag cagtttatga
     7681 gctggggctg gcgtgtgcca tttatcttct cggcggtgct ggtcattatc ggcctgtatg
     7741 ttcgcgtgtc gctgcatgag tcgccggtgt ttgagaaagt cgctaaagcg aaaaaacagg
     7801 tgaagatccc gctgggtacg ctgctgacca aacatgttcg cgtaaccgta ctgggtacgt
     7861 tcattatgct ggcaacctat acgctgtttt acatcatgac ggtctactct atgaccttta
     7921 gtaccgccgc cgcgccagtt gggcttggcc tgccgcgtaa cgaagtgttg tggatgttga
     7981 tgatggcagt tattggtttt ggcgtgatgg tgccagtcgc tggattactg gctgatgcct
     8041 ttggtcgccg taaaagcatg gtaatcatca ccacgctgat catcctgttc gcgctgttcg
     8101 cctttaaccc actgctcggt tctggcaacc cgattctggt ttttgccttc ctgctgctgg
     8161 ggttaagtct gatgggtctg accttcgggc caatgggtgc gctgttacca gagctgtttc
     8221 cgacagaagt gcgttacacc ggagcatcgt tctcttacaa cgtagcgtcg attctcgggg
     8281 cttccgttgc gccatatatc gcagcctggt tgcagactaa ctacgggcta agtgcggtgg
     8341 ggttatatct ggcggcgatg gctggcttga cgttaatcgc cctgctgctg acccatgaga
     8401 cgcgacatca gtcgttgtaa taagagtgaa aagcctgatg cgctacgctt atcaggcctg
     8461 aatttccata caatatattg aattcgcaag gatttgtagg ccggataagg cgttcacgcc
     8521 gcatccggca tgaacaaagc acactttgcc aacaatctaa atctgtttaa ttgccggatg
     8581 tcagacatcc ggcaattatc ttcacttctt catctgcgac aaaatcgtcc ggcactgatt
     8641 agcctcccct tcggaaggtg agatcagtgc cagcaacgct gccgctggcg taaccagcgt
     8701 tgccagtgcc gcagcaacag caccacgggc aatcaacggc ccggctttca ccccagcctg
     8761 aggattttta aacgtcccac gcacatacag cggcgaacgc agtgtgataa tccgaatacc
     8821 tttactctcc ggatcaatag tcaaatccag ctgttccgaa gcaaaacttg ccgtgccggt
     8881 aacgttaatc aacgcgttct cagtatcgaa agcaaaaatc tgcgggcgtg ccacgccgtt
     8941 ggcaatattc agattcgccg ccgcgcagtt cacccgcacc tcatcgtcac caaatatcgc
     9001 accgacaatg tagttgccga cattcagccc aacaatctcc atcaggttgc ggctcaccag
     9061 cccgtcattc atcaacagtt tcaggttgcc gttactgttg cctaaaagtg ccgccaccga
     9121 gttaccgcta ccgcgcagtt ccgcgtcacc gttcatttcc cccagcgtct tctgcatcag
     9181 ttccacatcg ggcatcagtt ctttcagttt cagtcgacga gcctgaatat ctgcccgccc
     9241 ctgcatcggc tttttatcgc cttccagatg aatattcgcc gcaatgctgc cacccgcaat
     9301 gccaaatttc agcggttgca ggcgcaagtc agcatttttg aggatgatat gagtagaaag
     9361 atcgctaatc ggcaggctac tgccatgctc aatgcgccgc cctttgaagc gaacatcggc
     9421 atccataacg tcccatttat cggtttcgaa gcggtcataa ggcagcactt tgcccgcagg
     9481 ctgaacgctt ttttcgccct tcttctgttc agaccgtttc gacttttctg cccctttccc
     9541 ggaatcaacg ccaatcaacg gtcccaggtc cgccagccgc aattgccgcg actcgacatc
     9601 accttccagt tttggtcgtg gcttgccggt ggtgtagacc agagaaccgt ggatatcgct
     9661 atcgccaatt cgcccattaa agccgcgata atcaaagacc gacgattttt cagtgtcgat
     9721 tttcgctacc agccgaccat ccgtttcaaa cggcggggta tcgggcagca gaacgcccgt
     9781 cagttcatag agatcaccca gtgaatcgcc agaaaattta agccgtaaat cgacaccgcc
     9841 catcttcatt gggtcattca cgacgccatc aaaagcaaca cgggtattac ctgaacggaa
     9901 atcagcctgt accggaaacg gcgtcccttc gccacgcagc gccagcatac cgcctatttt
     9961 tcccgtaccc gtgagcggtt caccgttata acgtccctgc gccttcaggc caaaaacgta
    10021 atcgcccacc ttttctttat ccgctttacc tttcgatcca gtaacttcgc tgaacggcag
    10081 cggcttgcct aagggatcaa caaaaatctc cagatccgct ttgcttactt tgtcatcaat
    10141 ggcgatccgc ccttgatcga aaagaatatt atccagccga aacgaccatg ccgacggctt
    10201 tgcattcgcg tctttgttat catcgttggc gagattaaac gtccagttat tgttcttttc
    10261 agagaggcga atcaggcgcg cgtcgggctt ttcgagcttg atccacggca gccagaccgt
    10321 tttggtcagc agcgccagcg gggccagcgt tgcttctacg cgtggcaaat gcaccatcgt
    10381 gacttcggga atatccggtg gattgccaag aatgatgtct tccgcgtgta catggggcca
    10441 cggcacccag ctgcgccagc cagtttcttg cttttgccgc tcccacacca cgcccagatc
    10501 gccacggata gcgaacggac gattcaactc cgcagagact ttctggttga tggtcggttt
    10561 gagtcgattc cagtcaaaag ttgcaatcaa aatgatcgcc acgacaatca acaacaagaa
    10621 agcccctgaa atcgcagcgg ttattttgcc tgccttgctc atcgttcgct tcctcatatt
    10681 cttcctgtgc cagtcctaaa gatagtccag ccaggcggaa aatgaggcag cttatagcgc
    10741 cagaaccgcc gtattcagcg tttctatcgg tgccgggcgt gaaagaaacc agccttgtgc
    10801 ggcgaatgcg ggcgagttct gtacatcacg ccactcttcc ggcgtttcta cgccctcgac
    10861 aatcaccccg cgacaatagc gattcattag atgtaaaagc tgagaaaaga gtgtgcgtcc
    10921 ttccggcgac tgacgcagca tcacaaacag ttctcgcgcg attttgatgt agtcataacg
    10981 cacttcactt agtgcagaga aatttgccat cccggtacca aaatcatcca gccacagcgg
    11041 gccaaattca cacatcgagg caaaggttga atctttcggc agacggatat gctccaccag
    11101 ttcgaaacgc agccagggaa gacgctcaat ctggcgcagg atttttggtt gctgacgcag
    11161 ggcgatgagc gtaggaccat caatattgac cgatgccagc aggccgtgct ctataaagaa
    11221 gtcggctttt tgcgccagca aatcaatctg ctctttcaca acctccatcc gatggctgac
    11281 ggtgatttca gtaaaatagc gatccggcgg caggcgttgc gaagggttca agggatgcgt
    11341 gaccgccgtt aatagctcca cggccattaa ccgcccgcat gtttgataga tcggctgcca
    11401 ggtgtaagca cgctcacact gcaaccaaaa acgccgttcc tgcaagctct cgatgcttgc
    11461 ttcagggttg cttattcgct ggataacctg ccttatcatc aaagatgtcc tgtgtttaaa
    11521 agtgatgcag cccggactcg acaaagatta tcggcgcgca agggcagaac tttaccgcct
    11581 ggccggggta aatttcccca ctccacaacg gaatctttca ggaatacgtt ctggctcaaa
    11641 aaaataatgg aacactgttt taatatggtt gaccagcaaa ccaccacagc gcaaactaac
    11701 gctaattttt tacagatcag gttcacgact atgtccaaaa agattgccgt gattggcgaa
    11761 tgcatgattg agctttccga gaaaggcgcg gacgttaagc gcggtttcgg cggcgatacc
    11821 ctgaacactt ccgtctatat cgcccgtcag gtcgatcctg cggcattaac cgttcattac
    11881 gtaacggcgc tgggaacgga cagttttagc cagcagatgc tggacgcctg gcacggcgag
    11941 aacgttgata cttccctgac ccaacggatg gaaaaccgtc tgccgggcct ttactacatt
    12001 gaaaccgaca gcaccggcga acgtacgttc tactactggc gtaacgaagc cgccgccaaa
    12061 ttctggctgg agagcgatca atccgcggcg atttgcgaag agctggcgaa tttcgattat
    12121 ctctacctga gcgggattag cctggcgatc ttaagcccga ccagccgcga aaagctgctt
    12181 tccctgctgc gcgaatgccg cgccaacggc ggaaaagtga ttttcgacaa taactatcgt
    12241 ccgcgcctgt gggccagcaa agaagagaca cagcaggtgt accaacaaat gctggaatgc
    12301 acggatatcg ccttcctgac gctggacgat gaagacgcgc tgtggggtca acagccggtg
    12361 gaagacgtca ttgcgcgcac ccataacgcg ggcgtgaaag aagtggtggt gaaacgcggg
    12421 gcggattctt gcctggtgtc cattgctggc gaagggttag tggatgttcc ggcggtgaaa
    12481 ctgccgaaag aaaaagtgat cgataccacc gcagctggcg actctttcag tgccggttat
    12541 ctggcggtac gtttgacggg cggcagtgcg gaaaacgcag cgaaacgcgg gcacctgacc
    12601 gcaagtaccg ttattcagta tcgcggcgcg attatcccgc gtgaggcgat gccagcgtaa
    12661 acgcagcata tgcaaaaggc tggataaaga gttacccctc tatccagctc atccataaat
    12721 acctgatggt tgtgctaact catcaccatc aggcattact gtgcaggtgg aatatccgtc
    12781 acttcaggat gtacgtcatc cgtggcgaca gaggtggtcg cagcggcggt agatggggcc
    12841 atgatttgat cccaggccgc ctgcaacgct ttcatgttaa attccggctc gcctttcggc
    12901 tgcagcagta ttaacgccat atcattcgac aactgctgac gcagatcctg atttaacatc
    12961 tccacagtca ggctgttaag gaaatcctgc cgcagtttct gatactgctc cggcgcgata
    13021 tcgacgacct gattttgcaa cgaacgcatc cgctggccca tcagaatatc ggtatcagcc
    13081 cgtgcatagg cggcaaacag tttctgcagc tccagtttct tttgcgccac taacgcattg
    13141 aactcttctt ccggcagacc tttatcgcga accttcgcca gttcacgcgc caccagatta
    13201 aggttgctgt tcagcttgtc gtttggtgat tcgatgttga tggcacactg cgcacgcaga
    13261 tacagcacac ggcagtcaaa tccaagaccg atgtctttgc tgttactggc gcttaacgct
    13321 tgctgaacat gccagaacag cgcctcacgg gccaggtccg cacgccagta gcgcagcagt
    13381 gcggctgatt cacgaatcgg ctgccacggc gtatcccaca tgatagataa ccggtcctga
    13441 cgcaccgcgt cagtcataat actcaccgct tccgcacgca gcggagaaag cgtcggcacc
    13501 ggagccggtg tttcacgttt gcctttcagt tcgccaaacg ttttgttgat ttggtcgacg
    13561 accgagcgcg catccacgtt tcccaccacc agcagcgtca ttgcatccgg ggtgtaccat
    13621 ttctgataga aatctttaat tttttccgct tcaacgggtt gtttcagcgg atcggcagga
    13681 tcgtgaccta acaaggttga ccctttcaga cgatagcgcc accagccctc tttagtatcg
    13741 gcaggccagg ttgccaccat gtcctgactt tgcagcgcgt ggttgatggt ttctggcgtg
    13801 atagtcagtt tgccagtggc atttgccaga taagagagcg cctctttcag caaatcgtta
    13861 cggttattgg gcaaactcag attaaacagc gtggtgtcat aagagacaat taccggcggc
    13921 atcgggcgtt tagggtcgat cccctgctgc cacaatgaac gcgcctgtgc tgcgtcaagg
    13981 ccaccgcttt gcgttagcgc aatacgaggg atggcgtgac tgtaaccgct ctgttgtgta
    14041 ctttcggcga gcgaaccggt attaaccagc aggcgaattt caacacgatc gctgggacgc
    14101 tggggggtgg tcagcacttg ccactgtaaa ccgttggaaa gcgtcccctg ttgccatgct
    14161 ggatcaggct ggagcgcatc tgcctgcaca tagccagcag tggccatcat cagcaaaccg
    14221 cccgctaaaa gtcgaatttt tgtgccctgc atgtgaaccc ctgatcaact atcctggtaa
    14281 taaaaaaact gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc
    14341 gcaagaacat gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa
    14401 gttcaggcac aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca
    14461 taagtgatga gattaagagg ataattcgtg cgttttgcca tccggcgcac gattattcag
    14521 cgcatcgtcc agttttttgt ggtccagttc tttcacccac ttagcaacga caatggtcgc
    14581 tacgccgtta ccgaccaggt tagtcagcgc acgagcttct gacataaagc ggtcgatacc
    14641 gaggatcagc gccagacccg ctaccggcaa atggcccacc gcagagagcg tcgccgccag
    14701 cacgataaag ccactacccg ttacccctgc cgccccttta gaagaaagca gcaacacgat
    14761 taacagcgtg atttggtgga cgatatccat ctgactgtta gtggcctggg cgataaacac
    14821 cgccgccatt gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat
    14881 gaccagcccc accaccgatt tacggcagcc gagtttctcc atcttgtcga gcatacgcgg
    14941 cagcgccgac tcggaagatg aagtccccag tacaatcagc agttcttcac ggatgtagcg
    15001 gataaatttg aagatactga aaccagtcgc tttagcgatt gaacccaata ccagcaccac
    15061 aaacaggata caggtaatgt agaaacagat aatcagctgc cccagttgca ccagtgtgcc
    15121 gacgccgtat ttaccgatgg taaacgccat tgccccgaac gcaccaatag gtgccagacg
    15181 catgatcata ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatga cgttaaaaat
    15241 cagttggcct ttgctgccca gacggtggag cgcaaaacca aacagtacgg caaacagcag
    15301 cacctgcaga atgttaccgc tggcaaatgc gccaatgacg ctcgccggga tgacatccat
    15361 aatgaaggcg acaatgccct ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc
    15421 atcaagcgtt gccggatcga cgttcattcc ggcaccaggc tgcacgacgt taacgatgat
    15481 aagaccaata atcagcgcga tggtactgac aatttcaaag taaagcagtg cgactgcgcc
    15541 ggtacgaccg accgccttca tgctttccat gcccgcaatg cccgttacga cggtacaaaa
    15601 gatgacagga gcgatgatca tcttaatgag cttaacgaag ccgtcgccaa gcggtttcat
    15661 ttgctcgcct atttcaggat agaaatggcc aaggagaata ccaatggcta tcgctgtcag
    15721 gacctgaaag taaaggcttt taaacagaga ggttttcata gggtgtcctt tagtaaaacc
    15781 acaggtcttg taaggttatg gggtacctgc ggccttaaaa taacacccag acaacatcac
    15841 agaaatgtac ctggatcata attgaaacaa aaaggttaaa aagtttgagc tggctcgcac
    15901 aaaccagcac tttttaaagt tttgtaatca gtttggggta gctacttttc ttccaggtaa
    15961 ctctcttcga agatttcaat agggagtgga cgagcaaaaa ggaagccctg ggcaatacca
    16021 acgcccgctt ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt
    16081 tgtaagttca ggctctgcgc cagcatgata attgcagcaa tcatgctgct atcttccggc
    16141 aagccttcaa caaacatttt gtcgattttc agaacgtcga ttggcaacga tttcatatgc
    16201 tgcagctgac gcagccctgc gtagcccatg ccgaaatcat ccagcgccac ccgaactccg
    16261 gcattacgca gcggacggag gattgccacc gcagcatgag ggtcgtcaat acgtcggctt
    16321 tctgtcactt ccagaatcag tgttcccggc tgaatgcgat agcgggttaa cagttccagc
    16381 atatccgcca ccatattcgg gtgcatcagt tgcagcacag agaggtttac cgacaagggc
    16441 agcataatgc cgcgctcttg ccaggctgca agcaatcgac aggactcttc cagcacccag
    16501 tgaccgacgg taaccatcag cccacagcac tcaatgcgat cgattaagcc atccggcagg
    16561 tcccaactgc catccggttg ctggatacgc agtaacactt ccgcactgac cagtttaccg
    16621 ctggtcatct cgacctgtgg ctgtaaccaa atagcaaact gatgattttc cagtgcatta
    16681 aggatatcgc tctcttccgt caaccgcttc tgggcggctt ccatctgctg cggatcaaag
    16741 aactgaatct gattcttgcc tttatggcga gcggtaaatg ccgcagaaat agcgcgactg
    16801 taaagctgtt cggcggtgag atcgccgtag aacatcgcca cgctaatgct acagtgcgga
    16861 cggagttgaa tacgttcaat cggcaggcgc tcgctcatga tagtgagcac ttgctgaccc
    16921 aaagtgattg cgtgccacgg ttcatgtaca ccgttggcaa tgacagcaaa gtcataaccg
    16981 ctaatctgcg cgaggatcat acgtggcgac agtaccgatt tgagtttttc caccagcgtc
    17041 agcagcagaa tttctcgttg cgcctctttc agcacgcccg cagtatcacg cagagtttca
    17101 caggtgatga tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc cagcatctcc
    17161 atcagcaagg ctttgttcgg caaatccgac accgggaagc gcatcgcatt ttcgttctgt
    17221 tcttcataat ggcgctgcag caattgctgg ttgaggttgt aactgcgcac caacataccg
    17281 atttcatcgt cctgatgcag acgcggtaat gccagttggt gaccaacaag ctcctgggct
    17341 gggatggcgt taagttcgcg agcaatattg cgtaacggat gcaaaatcag gcggttaatg
    17401 caccagctaa tcgccaccgt caatattaac gacaaaagta agtaaatggt cactaacgtt
    17461 gagagggtgc tcatcacgaa cttatacata cggaaggaat ccgcctgtag caccagatag
    17521 gcaattggct gcggatttgc cggacgttcg agcgagtaga cgcccagcga gatttgaacc
    17581 ggtagctcaa acaggcgagt aaccattacc ggcaccggac gctctggaat aaaactttta
    17641 cgcagcgcct ggaactggtt aggcagcact acatcggcac ggctgaccac gcctgccggt
    17701 ttaatgctgg caagaatagc ttccgcttcg ggaatatcac ctttcaaaat ggctgaagat
    17761 aaaggttcgc ggacagagcg agcaatgctt tccagttgcg tagccgtgtt atagcgattc
    17821 tgctggacca gatggaacag caaaacggtg caaaaaataa aaacgaacac caggacaacg
    17881 gctgccacca ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa attgtctccc
    17941 cccggaaatc ccagattgcc gcccgagtat acccgatcgc agcggcatta agagaggcgc
    18001 tatctgaaaa cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa atccatgtca
    18061 ccctgccatc cggcggcgga ataacgtacg tagagcagga aatggctggg ggcgtaatct
    18121 ttcgcctgct ggatatcaat tgccgtgccg acaaaccagt tggaagtaac acgtcgttca
    18181 agtaatgccc gcgccgtata gccgaagccc tgactgctgc cgccatcgtt ggattgccgc
    18241 gcagcttctt cctgccagtc ggtcgggatc agattcatca gcggataacg cggcatggtt
    18301 ttggtgcgtg aatgcgacca cgagccagac gcccccagct cccacgacca gttttccgtg
    18361 cgctcccgcc acatcaccgg tatggcaaac gacaggtatt cctgcgggct gtagtaaccg
    18421 ccctgaccga gtgagtagcc actcaggtct ttgtcgcaat gccagatcat gttgttcagg
    18481 ccgattgtga cgcggcgatt gttctggttg atgaccttat agtaatagcc cgtcatccag
    18541 cgcacacgcc agttatcttc gacattttta ccggttaact ggtcgccact aagcgatgcc
    18601 cagacgccgt ttgcttcacc tttatcgtag ctcaggctta gccccacgcc gtcggcacgc
    18661 acgccgcccc attttttccc ggtattgctc ggggagtctt tttgcccacc aaaggccagc
    18721 aaagaactgg agatgggacg acggtgggcg ttaacggtgt aacccagcgg cccgatatca
    18781 tcgctgtaac tgatgccgcc gaccacatcc accacgttga agcccatcgg cgtggtgccg
    18841 atatcccagc tccagacgtc atttcgccag ccgaccgcca cgctggcacc ggaatccgac
    18901 tggctgcggt tgccgctaca gtcccgtaat gtacaggtgc cccagttgtc atcccatttg
    18961 ccatcggcat tagtggagaa actgccgacg ttcatattga cgaaatcact gcgaaagaac
    19021 atccgcccgt cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg
    19081 gagtaaccac cggtgccgct cgaaccccaa taatcatgct cgagggtgac gttaagatcc
    19141 tgctggcgat agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc
    19201 tcgtcgttac gggtcagtcg ggtaaaggtg tcgttatcct gcggacgcgt cgtagtcaca
    19261 ccggatgcga ccatggcgtc tttgtaggtt tccagcgcct gctttggatc gcccgcctgc
    19321 gcttcaaact tcgcaccgtc gcgcagcacc atcgcgcttt ccatcgacgg tggctgagat
    19381 tttgcctgcg ggatcaactt attaaacgtc tgctgcgccg ctgcggtatc tccaagctgc
    19441 gcctgcgcca gcgccacgcg ccgctgtgtg ttcagcgagg cgttatcagt agcgggcagt
    19501 ttcgccagct ggctacgtgc cgccgctgtg tcaccggcag caatatccac ttccgtcaga
    19561 ccaagaatgg catcggcgtt agttggctcc cgcgtcagga cattctgata tgcagcgcgg
    19621 gcggcggtgt aatcacgtcg ctgcagcgcc cagtcagcca gcgtgaggtc aatacgcgtg
    19681 gaaggtggtt gctgacgcag catcgcttcc gcttcagctt ctttgccgct ttctcgcagg
    19741 cgattagcgg tttccagcac ctgatcgctt tgcagtcgat taaccagctc ctgaatattg
    19801 ctgctccact gcgcacgcgg caggctgttg atatgcgcca gcgccgctct gtcctggtca
    19861 tgaccagaga ggtacagccc gtaagcgtaa acctgctccg ggtcgttcgg cttctgctgc
    19921 gccagattgc gcattaacgt atcggcctgg ctgcgttgtc cggcctgcca gagatcctgc
    19981 gaaagtcggt aagtaatcca tacgctgccg gggtccagcg ccagtcgttg ccgctgaagt
    20041 gctgccgcct gcgcccattt gccctggttt tccagtgcct ctgcctgctg tgccagacgg
    20101 tcgttttgca ggctgcgttc gatatcatca atgctacgcc gctgactggc agagagtgag
    20161 gcgataaacg cttcagcttt ttctggcgat tgctggcggt aaatatttgc cagcccgcgc
    20221 acggcgttag tgttgccgct gtccatacgc aaggtctgct gataataacg ttcggcggcg
    20281 ggataatctt ttcgcgccat cgccacatcg cccagcccca gcactgcata actgtcggtg
    20341 ttatcgacat tacgcgcctg ctggaacagg cgttctgccc ggtcaggatt attggctttc
    20401 agcgcagcat cgccctgctg gatcgccagc cagtagcggt ttactttcag cagactgttc
    20461 catttgtcgt tgttgctgct gtgcggatcc agtgcgaggg ctttttccag attcgccact
    20521 gcattggcgc gatcgccttt ctgtgaatat gcctggccca gcgcccccag agcttcactg
    20581 tctttcgggt ttgcccgcac tgcctgttgt agttcgggaa tggctttacc cgccatacca
    20641 gagtccaccg ccgctaaacc ttgcgcacga gcgcggaaag caggatcggc cagctgtttt
    20701 tgctgttctg ccagttgcga ttgcgcagcc gccacgctat cgccatcact aaagatagaa
    20761 agatattttt tcagcgccga cacactggca tcactgacgg gcatgtcttt aatctgcccg
    20821 taccagattt tagaggcccc ttcgcgcccg gcgttcgatt tcgccatctg ttccaggacg
    20881 gcaaaacctt cgtcacggcg atcgctacta aacagcaata gcgccagatt gttttgcagg
    20941 cccgtattgc ccggtgcatc cgcattgatg cgttttaact gattaatcgc ttcgccacgg
    21001 cgagccggaa ttttcgccac cgtactccag tactcgacag caatgtcacc ttccggcggc
    21061 gcaccgttga acagtttgtt gtaactcgcc acagcttctt ccgcatgacc ggtagtcgcc
    21121 tgcaatcttg cctgttgcag tgcctgacga ccatccggcg tggaaagtag catcgtagtc
    21181 cgcgacgatt tatacgcatt tgaactcggc gctaactgcg acagccgatc gagctgtttt
    21241 tgcgcgccat caatatcgcc ctgacgtaac aaagaacgga aacgggcggc aacgacgtcc
    21301 gggttattcg gatcaataag ttccagccga tacaacgact gctgcaccag gtcttcacga
    21361 tgggtcgctt cgcctaaccg aacttgctcc agcaactgtt gctgagcggt tggtgctgcc
    21421 tcgaccatcg gcatgacggc cagaccgagg gaaagcgtga atatgtttag tgtgaatttg
    21481 cgcattcctg gccccagtca ggtaataact caccttttgt cgagaagcgg aaacggtgtt
    21541 gatcccagcc ttgtccaaac agggtcagca catagttgta ataggcatcg ctgccgggaa
    21601 agttatcggc cacgcgctgg cgctgaacgg cctgcgcatc acggttttgt aaaaagggca
    21661 gcatggcggc agaaaaaccg accggtcctt taccctgcgc tttccccgta gccacatcca
    21721 ctttttccgg cggataaccg tttttctcag tgaatgtcgc catcggttta aaccggttga
    21781 gcatccgcgc tttctgcgga tcgctgtcag gcatcatgcc tacccacatg taaacgcgga
    21841 tagcgtcgta gctgctgatc aatgtttttt cggcttttag ctgccagcct ttgtctttct
    21901 catagcgcac ccagtctggc gaaaagcctt tcggggcggt ttccagcaat aaacgttgat
    21961 tggtttcgcg cagcgtagtc cacggcgcgc caaagcgggt gaaatactgc gccagcgtcg
    22021 gcggcaggta gctggggtta aaacgccagc tgttatcctc agcaaaaccc actttgcccg
    22081 gtaacaacat ggaacccagc ccaggcaccg tcaccacttc ctcccgcgca atacgtttta
    22141 gcaacgcgct gccgatgtcg gtataacgct gctctttcca caaacgcccc gcctccagca
    22201 acgaccaagc catccagaca tcaccatcgg aggccgaatt gctgtccagt acttcccact
    22261 tactgttctc tttcttgccc cacagccagg cgggcaaacg ttcttttaaa gaaccctgag
    22321 cgagattgtt ctgcgtccag tcgagaatat tatcgaaagc tgcacggtcg ttagccgcca
    22381 gggcaaagaa catgccgtaa ctttgccctt cagaggtggt gattttgcgc gcgtcgctgg
    22441 ggtcgatgac gcgcccttcc tgactgatgt aatccttttt aaactgctcc caggcaggcc
    22501 aggtacaggc tgcctgaaca ctaaaggcag ccaacagcag catcgtcacg agtccactac
    22561 gcaacacatt catcttcaat tactcgttat ccgggttaag acgacgacgg ctgataatac
    22621 gcagcaaacg ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca
    22681 gcaccgccag cagaatcgga tggtttgcca gcgcatacca caagcgctcg aaccacggca
    22741 gatgacctac gtaataaacg tcgccaacac gtaggctgtt gataccggac tcgcggatca
    22801 ccgcgaccga accgaacatg gtggcgcgtt tgccgctatc gttcaccgca tcgttaagca
    22861 tttcataacc gcgtgggcta tctgccagca gcgcaatcac gctgcgctgg tcgttatacg
    22921 gcgactggaa gccaatcacc gccgccatcg caccggaaga ggtcagcgtt gactgggttt
    22981 ctgccgcgcg atcgctctcg tccggcacaa tgccggggaa cggggtctgg cgcatcggtg
    23041 ttttcaccca gctttcggtc gcctgcacca ataggtcgat ctgcttatcg tctttcagtt
    23101 tgtccgggat accaccgatg atcatgatgt cggcatcttt gccctgaatg gtgctgccat
    23161 catcggtcac cgtcagatta atcgccggga agcccgtctg tgcgccgata aaaccaacag
    23221 tattcagcaa cgtttccatc tgtgcttcgt taggcgtttt cggcatcacg gtgatggttt
    23281 gcgacagatc cgccatccgg ctgaacggga agcccgcgtt agcaaaggcg cgtagatccg
    23341 gcatcgggat gaagtggtaa tacttcgaga agtcgatggt ggagtcgtca ccaatcacca
    23401 catgattctg caccggctgg aaggtaatac agttatccac cgaaccgcca ggcatcgggt
    23461 tcatatactc aaagtcgaag cgcagctggt tggtcgcgcc cagtttcagc gccggaatag
    23521 agacatccgt tttgccatcc agcaaacctt gtaataccgg aatccgcagc agcaggcggt
    23581 tcgcctcctg tttgctgctc aggttgaagg attgcaggaa ctggttattc aggctgatat
    23641 ccatccgcga actgtctttc accggcggca tggtgtagcg gtaattaata tccatatcaa
    23701 tgccggtact gcgcatcagg tagagatctg gcggcaggtt tagcgaaaca ttaatcgctg
    23761 ctggctcaag accgctggat tgtaactgtt cttcataggt tttcagttca ccaaaggtga
    23821 ccggacgatc ggtacgcacc cagttcggcg catcgtacgg cttacgcggt agcagcggtt
    23881 tcacttcatt cactaccacg ctttcaccac ggaacagaat gttaccctga gcgatacctt
    23941 tcgctgcctg caacaggtct ttgtcgtcac gaccaaacac caccagcagt ttgacgtaag
    24001 gattctgcgg atggttaatc atctcaatca ccggggcttt taccgccgga tgatcgcgca
    24061 ggaagtccgg ccgtttgtcg ttggttgcaa agacaatggc attacgatcc ggcagttggt
    24121 tatagagcac cgggaagttc tgcccacgcc agccagaacg cgaaccaaac cacgaggcga
    24181 caatggcaga tgcttgttgc agcccaacat ccggcgcacc cgcaaagacc atcggcaagg
    24241 tgttggtgcg gttatcacgc gggtcaaaga atggcaccgg gaagtgtgac aggtcattct
    24301 tcacattcag ggtctgatag gtcagatcca gtccactgct ccgcccaaca tccagccaaa
    24361 gcgtggtgct ggccgggttt tcgcacacgt cctgataatg gccgacaaac tccagccgca
    24421 cacggttgaa gtcggtaata aacagtggat taatgggcat ttgcgccagc gtttttttac
    24481 ccaactgttc tttggtcact ggcagcacgc ccatcagctc atcattgaga taaaccttta
    24541 actgcgactg gacaggcagt aacgatggcg atggcgtgta ttcgaggttg agcatcgcct
    24601 tcgtcaccac ttcatcgctg cgcataccaa actcaatgct gccgttcggg ttaatgccac
    24661 gtagcaccat gctgcccggc ggcggcgcaa tttgcgcaaa ggtcagcttc acatcacgcg
    24721 aaggaccgtt ctgcgccacg actggcgcat cagcgccctg cacgcccggc atcacttgcc
    24781 ccacctgcgg attttgttcc gtctgggcgg ctacagctgg ctcagcattg atcagtggtt
    24841 gcgttgctgg cgtcgcctgc gtcatgaaag aggggaacgc actcatcccc atagccactg
    24901 cacaaatcca gaatagtttt cttttcatcg cgttatcatc attgttgagc caaagcctga
    24961 tccgatggtt gtgccgtttc gctccgctcc gggcggcgcg gaataaacga tacaacccag
    25021 gaaaccagag aagtcagcac acggaatatg cccttcaccg aagaaggcgc aaactccgcc
    25081 agatggcggt agccacggaa gccgagcttc agaatatcca gcagactttc cagcggctta
    25141 tcttccgggt agctgtcctg ccagagcgcc catgtatccg cacgggcaaa cgtacactgc
    25201 acaaaatcga tatgttgctg ggtggtgagc ggcattaatt tcagcccaac ttcattaccc
    25261 atcacgcgcg ccacctgggt cgggaagacg tattcctgct gaccgcgttt aagcaacaga
    25321 ttcactttct gcccttccag aatctgcgcc tgaccgttga tcttgatccc caaaccaccg
    25381 tcggagaaat cctgaacggt acacgagaag aggtgaccat cttcgcgggc aattgccgcg
    25441 ggcatcgtca tctccacgcg gtgcgatcgg cgtacctgtt tgctttctac cgataccgca
    25501 actgcgccgc caagaacaat caggttgtag aatacccaca ccatactgac gaccacggtg
    25561 agcatctcgg ttggcgggcc atagaagtag cgccagatgc ctaccgcaac gcccaccagg
    25621 ttgagcagaa caaggaagat gtagggccgc gagatcaccc agtcgacgta ctcttcttcc
    25681 accagtccac ctttggcggt gacgttaaat ttgcctttgt gcgggttaat cagcgccacc
    25741 agcgtcggtg gtgcgatata ccacgccagc accgtttcgt agatttcact ccagaaagag
    25801 tggcgatatt tgccctggat cttggagttg gtcaggctgg catggatcat atgcggcagc
    25861 acgaacaggg cgatcatcaa cgctggcgca tagatgatgt aggcatgaag cagcaggaac
    25921 gccagcggcg cagtcaggaa gatcagccgt ggaatgcccg acaagaagtg gaacatggcg
    25981 ttgacgtagc acagccgctg agcaaacttc agccctttac cggtgagcgg gttatcgaga
    26041 cggaagattt gcaccatccc gcgcgcccag cgaatacgct gaccgatatg cgccgacaga
    26101 ctttcggtcg ccagccccgc cgcctgcgga atacgcatat acgcggaggt atagccacga
    26161 cggtgcaaac gcagagaagt atgcgcatct tcagtcacag tttcgacagc aatgccgcca
    26221 atttcatcca gcggcttacg gcgaatcacc gcacaggaac cgcagaagaa agtggcgtcc
    26281 cacatatcgt tgccatcctg caccagacca tagaacagcg tgccttcgtt cggtgtttta
    26341 cgaaaacgcc ccaggttgcg ttcaaacggg tccggcgaga agaaatggtg tggggtctgc
    26401 atcatcgcca gctgtttctc tttcaggaac cagcccacgg tcatttgcag gaacgatcgc
    26461 gttggtacgt ggtcgcagtc gaaaatcgac acgaactcgc ctttggcata tttcagcgca
    26521 ttgttgatgt tgcccgcttt cgcatgttca tgagtggtac gggcgatata cttcaccccc
    26581 acgttttgcg caaactggcg aaactcttcc ctgccgccat catcgaggat ccagatgttc
    26641 agcttgtctt tcggccagtc gatacccagc gaggcgtaaa tggtattttt caccacgttg
    26701 agatcttcgt tgtaagtcgg gacaaagata tccaccgacg gccacagcga catatctttc
    26761 ggcaatggca ccggctgacg attcagcggc catactacct ggaagtagcc gagcaccagc
    26821 acaatccacg cgtacgtttc agcgaagagc agaataagcc cgcacaccag gctgaccgga
    26881 tcgtcccagt tcagcgtaga ggtatatcgc caccagatat aacggcaaga aacagtcagc
    26941 gacagcacaa tcaacattag cgctgagaag cgccccggca tccgccgtac gatcagcgct
    27001 cccccccaca gcagcatcag gaagataaac tgcgccagcg ggttaaacgg ctgagtaacg
    27061 cagattaacg ccagaatcag cgagaaggtg acgatgatac cgaggatcaa ccgccgcgcc
    27121 ccggcactca aatgaccgag ctcttttttc tcatcaagat gctgtgtttt atggctaacg
    27181 cgctcaggca gctcgttcat ccattgatgg taacgtccac gaatattctg cagacctgaa
    27241 aatgcccgcc tgcgcggttt cggcgtttct ttgcgcgatg caccgatcag taaccagcat
    27301 gtttgaatga gataacggac cgggtccagc ggacgcggac gcgaggcgtt gatatgcgga
    27361 tacagatttt tatgttctgc gcgaatacgc tgccagcgcg ggtgctccag tggaataaaa
    27421 atccaggcca ggatcatcca gaaacagccg agcgtcgcgc tgaaagccga cgcaccgtga
    27481 cgacgataat cgcgataacg cccgataagc cgcgcgttga ccggcgggat aagcaaccac
    27541 cgggtcagga tactcatgat gcactcccga ctggcgtttt cagcccggaa tagttcaaca
    27601 ggcaccagtt cgccagcgtc aatatctctt cagccgccag cgcatcactg cgatattcac
    27661 ctactggttg cttagccgcc aggcattcag ccatcgcttc atcacgatga atgagcatcg
    27721 gcagtaatcg gcgctggctt tgcaaccaaa gctggtaaat atcgtcctga acctgactgc
    27781 caatacggaa gttattaatc aaaatatgtg cgccatccgg cagcgcttgc tgatgcagtc
    27841 ggatatggca gttggcatcc acattgacga ttgccagcga gtgatcgcac aaactcagca
    27901 gctggtgggt tatctgcgag gcatcacgcg gtaagtcgat taaaatccac tggtaacgcc
    27961 cgctggcttt tagttgctgt aagccggagc aaatatcgct cagccgggtt tgccagtgct
    28021 gtggattttc ttgttcttca atggataact gaccaaaagg cagcaaatcg agctgcgagg
    28081 tgtagcgcaa cccagcgtcg cgccagtcct agccatccag catagctctg gcccagccct
    28141 gacggtgggt aaaatcaaca ttaaatgaca ggcgcaacaa gttgtccggg caggcatcga
    28201 ccaccaggac attttctccc aacatttgta atgaccaggc taatgcggcg gtgatggttg
    28261 t
//
LOCUS       DAZGXS010000053        28053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGXS010000053 DAZGXS010000000
VERSION     DAZGXS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(283..1653)
                     /gene="lsrK"
                     /locus_tag="ACHKG5_002392"
     CDS             complement(283..1653)
                     /gene="lsrK"
                     /locus_tag="ACHKG5_002392"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP0868487.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSL
                     WKAPGL"
     gene            complement(1732..2685)
                     /gene="lsrR"
                     /locus_tag="ACHKG5_002393"
     CDS             complement(1732..2685)
                     /gene="lsrR"
                     /locus_tag="ACHKG5_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0868488.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2934..4469
                     /gene="lsrA"
                     /locus_tag="ACHKG5_002394"
     CDS             2934..4469
                     /gene="lsrA"
                     /locus_tag="ACHKG5_002394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP0868489.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4463..5491
                     /gene="lsrC"
                     /locus_tag="ACHKG5_002395"
     CDS             4463..5491
                     /gene="lsrC"
                     /locus_tag="ACHKG5_002395"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0868490.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5491..6483
                     /gene="lsrD"
                     /locus_tag="ACHKG5_002396"
     CDS             5491..6483
                     /gene="lsrD"
                     /locus_tag="ACHKG5_002396"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0868491.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6495..7517
                     /gene="lsrB"
                     /locus_tag="ACHKG5_002397"
     CDS             6495..7517
                     /gene="lsrB"
                     /locus_tag="ACHKG5_002397"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP0868492.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7544..8419
                     /gene="lsrF"
                     /locus_tag="ACHKG5_002398"
     CDS             7544..8419
                     /gene="lsrF"
                     /locus_tag="ACHKG5_002398"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0868493.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8443..8733
                     /gene="lsrG"
                     /locus_tag="ACHKG5_002399"
     CDS             8443..8733
                     /gene="lsrG"
                     /locus_tag="ACHKG5_002399"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0868494.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8790..9548
                     /gene="tam"
                     /locus_tag="ACHKG5_002400"
     CDS             8790..9548
                     /gene="tam"
                     /locus_tag="ACHKG5_002400"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0868495.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9552..10466)
                     /gene="yneE"
                     /locus_tag="ACHKG5_002401"
     CDS             complement(9552..10466)
                     /gene="yneE"
                     /locus_tag="ACHKG5_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0868496.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10663..12114)
                     /gene="uxaB"
                     /locus_tag="ACHKG5_002402"
     CDS             complement(10663..12114)
                     /gene="uxaB"
                     /locus_tag="ACHKG5_002402"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0868497.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12341..13759)
                     /locus_tag="ACHKG5_002403"
     CDS             complement(12341..13759)
                     /locus_tag="ACHKG5_002403"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0868498.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13898..14257)
                     /gene="yneG"
                     /locus_tag="ACHKG5_002404"
     CDS             complement(13898..14257)
                     /gene="yneG"
                     /locus_tag="ACHKG5_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP0868499.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14257..15183)
                     /gene="glsB"
                     /locus_tag="ACHKG5_002405"
     CDS             complement(14257..15183)
                     /gene="glsB"
                     /locus_tag="ACHKG5_002405"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP0868500.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15247..16635)
                     /gene="sad"
                     /locus_tag="ACHKG5_002406"
     CDS             complement(15247..16635)
                     /gene="sad"
                     /locus_tag="ACHKG5_002406"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP0868501.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16736..17617
                     /gene="yneJ"
                     /locus_tag="ACHKG5_002407"
     CDS             16736..17617
                     /gene="yneJ"
                     /locus_tag="ACHKG5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0868502.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17695..18810
                     /gene="yneK"
                     /locus_tag="ACHKG5_002408"
                     /pseudo
     CDS             17695..18810
                     /gene="yneK"
                     /locus_tag="ACHKG5_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18961..20151
                     /gene="ydeA"
                     /locus_tag="ACHKG5_002409"
     CDS             18961..20151
                     /gene="ydeA"
                     /locus_tag="ACHKG5_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP0868503.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20176..20841)
                     /gene="marC"
                     /locus_tag="ACHKG5_002410"
     CDS             complement(20176..20841)
                     /gene="marC"
                     /locus_tag="ACHKG5_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP0868504.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMSGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            21053..21487
                     /gene="marR"
                     /locus_tag="ACHKG5_002411"
     CDS             21053..21487
                     /gene="marR"
                     /locus_tag="ACHKG5_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP0868505.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21507..21890
                     /gene="marA"
                     /locus_tag="ACHKG5_002412"
     CDS             21507..21890
                     /gene="marA"
                     /locus_tag="ACHKG5_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0868506.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21922..22140
                     /gene="marB"
                     /locus_tag="ACHKG5_002413"
     CDS             21922..22140
                     /gene="marB"
                     /locus_tag="ACHKG5_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0868507.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22171..23070)
                     /gene="eamA"
                     /locus_tag="ACHKG5_002414"
     CDS             complement(22171..23070)
                     /gene="eamA"
                     /locus_tag="ACHKG5_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0868508.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23265..24452
                     /gene="ydeE"
                     /locus_tag="ACHKG5_002415"
     CDS             23265..24452
                     /gene="ydeE"
                     /locus_tag="ACHKG5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0868509.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(24493..24888)
                     /locus_tag="ACHKG5_002416"
     CDS             complement(24493..24888)
                     /locus_tag="ACHKG5_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0868510.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            24983..25954
                     /gene="ftrA"
                     /locus_tag="ACHKG5_002417"
     CDS             24983..25954
                     /gene="ftrA"
                     /locus_tag="ACHKG5_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFP0868511.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            26062..26157
                     /gene="mgtS"
                     /locus_tag="ACHKG5_002418"
     CDS             26062..26157
                     /gene="mgtS"
                     /locus_tag="ACHKG5_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0868512.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            26157..26261
                     /gene="mgtT"
                     /locus_tag="ACHKG5_002419"
     CDS             26157..26261
                     /gene="mgtT"
                     /locus_tag="ACHKG5_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0868513.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26376..27266)
                     /gene="dgcZ"
                     /locus_tag="ACHKG5_002420"
     CDS             complement(26376..27266)
                     /gene="dgcZ"
                     /locus_tag="ACHKG5_002420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0868514.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(27521..27913)
                     /gene="ydeI"
                     /locus_tag="ACHKG5_002421"
     CDS             complement(27521..27913)
                     /gene="ydeI"
                     /locus_tag="ACHKG5_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0868515.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         7157 a         7052 c         6712 g         7132 t
ORIGIN      
        1 atcatcatca tatttaacaa agaatagcac caattgctaa aaatcgaagt ttattaaacc
       61 ccttttgcat ccattaatgt ctttaggaat attcgttata aaataagggt tatgcttaat
      121 gctttaagaa aaatagcaat ttccccttga atatagtact ggtgatgaaa cgatgaatct
      181 gcaagctggc atttaacaag ccagctctaa aagaagggaa ataagaataa ctatactcaa
      241 aaactaacag ccacggtcat catgatgtgg ctgtcaatga aactataacc caggcgcttt
      301 ccataacgac gtcgtcagtc catgatcaac cagccccagc tgatcctgat aaactgcctg
      361 ccacttatcg cgtgaatcct gataaagttc atgcttttcc gggtctggtg tgtgcgtccg
      421 ttcccagcga accaggcgtt ctccggtttc tgccattgat gaaaaaatac ggaacaatgt
      481 cgctttgtta catttatccg gatcaatgga caaattaata aaggaaggcg cagcgtgata
      541 ccaggtttta aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg
      601 cacccgactg gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat
      661 cagtttttct tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa
      721 gcttatagat tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat
      781 ttctgggtct gtcaccggcg cggctaaatt tacaacttgc tgccagaatg tgccgccaag
      841 aaccgcggtt tgtgccggac gcacaacgcc taaccccagg caaccaagct gcacgtcgcc
      901 tcctccaacg accaccggag tgcccacctt cagaccgcag agttccgccg cttgtgaact
      961 taccacgccc agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgcaggcc
     1021 agccatatcc agcaatgcag gtttccagtc acggatggtt agatcaagaa gtcccgtggt
     1081 gccagcgtta gagggatcca ccgcaagttc gccgctgagc atataggcca gccagtcgct
     1141 gatcatggtg atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag
     1201 taatctgggg atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc
     1261 aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg cgcgccgctc tggcatccac
     1321 attggcgcag gcccagatcg gtgttccttc attgttatat aaaacaatgc cttcacgcat
     1381 cgaacatgcc gaaacggcag cgatatactc cggggctatg ccggcgttgt gcagcgcctg
     1441 gcgcatacac tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg
     1501 aacgtccggt actgccagat gccgccactc cgcctgtccc actgctattt gattgccttc
     1561 caggtcgaat atcacagccc gaatacttcc ggtgcctgca tccagcgcca tcaggtagta
     1621 ctttgattct gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg
     1681 tctgttcctc tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg
     1741 taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat
     1801 tgcagcggca attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat
     1861 ggtcttcagc gcgcttaaag gtaagccaat cagctcgtta tgtattttaa tgtccgtgac
     1921 aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcggcc
     1981 aatcattaac tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcttg
     2041 ttgactaacg gccccaatgc cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac
     2101 atctttgaca caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa
     2161 cggagccgga ataatattca cactgcacgc cgcattaagc tgtccgatac ccgtcatata
     2221 agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact
     2281 taagcgttgc agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg
     2341 ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc
     2401 atccgcaagc ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgtgt
     2461 ttcatattcc agacagcctt caaagcgaga attaatctgc acgcgaatga tgccggactg
     2521 atgccctttc tccagtaatc gcgacacttt caaacgggtc aggccgagac gatcgctgat
     2581 ctcactctgg gtcagcccgt cgtggtagta aaaccacgcg atccgcgcga cctgttcttc
     2641 ttcacacatt ccctgttctg aaattaccga atcgttgatt gtcataattc attcttcact
     2701 ttgaacatat ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg
     2761 gcaaccacag tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagtat
     2821 aaattgtgat ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta
     2881 aaagcagaaa tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa
     2941 cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag
     3001 gggtcaatgt cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc
     3061 tcggcggcaa tggtgccggt aaatcgacgt taatgaagat tattgccggt attacctctg
     3121 ctgatagcgg tacgctggag attgggggca acaactacgc cagattaacg ccagttcatg
     3181 ctcatcagct gggtatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga
     3241 taaaagaaaa catcctgttt gggctggcaa aaaaacagct ctccatgcag aaaatgaaga
     3301 acttgctggc ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg
     3361 tcgccgatcg ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga
     3421 tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttactcgct
     3481 tgcaagagct gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa
     3541 ttcgccagat tgccgatcgg attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca
     3601 aaaccagcga actgtctacc gacgacatta ttcaggccat caccccagcg gtacgggaaa
     3661 aatcgctctc tgccagccaa aaattatggc tggagttacc tggcaaccgc ccacaacatg
     3721 ccgcaggaac gtcggtgctg acactggaaa atctgactgg cgaaggtttc aggaatgtca
     3781 gcctgacgct aaatgccgga gaaattctgg gtctggctgg gctggtgggt gccggacgca
     3841 cagaactggc cgagacgctc tatggtctac gtactttgcg tggcggacgc attatgctga
     3901 atgataaaga gatcaataga ttatccactg gagaacgttt actgcgcggt ctggtttatc
     3961 tgccggaaga tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct
     4021 gcgcccttac tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc
     4081 tggaacttta tcgtcgggcg ctgaatatta aatataacca accggaacaa gctgcacgga
     4141 cattatccgg tggtaaccag caaaaaatcc tgattgccaa atgcctggaa gcctcgccgc
     4201 aagtattgat tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcgcgt aatgatatct
     4261 accaactgtt gcgcagcatc gccgcacaga atgtggctgt gctgcttatc tcctctgatc
     4321 tggaagagat cgaactgatg gcagatcgtg tgtatgtgat gcatcagggc gaaattaccc
     4381 actctgcact gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg
     4441 atagtcagcg tcaggaggcg gcatgctgaa gtttattcag aacaaccgtg aaatcacggc
     4501 actgctggcg gtgctgctgc tgtttgtatt acccggtttt ctcgaccgcc agtatttaag
     4561 tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg
     4621 cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat
     4681 gtgcgcggtg ctgttgggga tgttactgaa cgcaggatat tcactacctg ttgcctgtgt
     4741 cgcgacttta ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct
     4801 aaagatccct gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt
     4861 gctgtggact ggcggtaaat ggattgaagg gttacccgcc gaactgaaac agctctccgc
     4921 cccgctgctg cttggcgttt cagcaattgg ttggttaacg ataattctgg tggcatttat
     4981 ggcctggctg ctggcgaaga cagcgtttgg acgcagtttt tatgccacgg gcgataattt
     5041 acagggcgct cgtcaactgg gcgttcgtac ggaagccatt cgcattgtgg cattttcgtt
     5101 gaacggctgc atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc
     5161 caaccagacc ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat
     5221 tagtttgctc ggcggttccg gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac
     5281 gcagatcgat agcgtactgg tgctgttgcg cattccggca tggtggaatg attttatcgc
     5341 gggtctggtt ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaact
     5401 taatctgcgg cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaacccgc
     5461 ttcgtcaggt aaaaaacggg aggctgcata atgcgtattc gctacggttg ggaactggct
     5521 cttgccgcac tgcttgttat tgagattgtc tcatttggtg caattaaccc gcggatgtta
     5581 gatctcaata tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta
     5641 ccgctgacga tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc
     5701 ctctgcgcca ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgatt
     5761 ctcctgacct tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat
     5821 accaaagtta acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgcc
     5881 ctgctgcttt ccgggatggc cggggcgacg gggtacgaag gtattggtgg attcccgatg
     5941 gcgtttacag atttcgctaa cctggatgtg ttggggctcc ccgttccgct gattatcttc
     6001 ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaacgtg
     6061 tttttgattg ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgtacc
     6121 ttatgtgcgc tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggtg
     6181 tcgtattttg gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc
     6241 gccgtggtgc ttggcggtgc caatatttat ggtggttccg gtgccattat cggcaccgcc
     6301 attgcggttt tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat
     6361 caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt
     6421 agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca
     6481 taaaggatat cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg
     6541 gcattgccgc aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac
     6601 tggttggcgt gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc
     6661 tgggcgttga tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt
     6721 tgattaataa cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg
     6781 atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg
     6841 actctgatac taaaccggag tgccgctctt actacattaa tcagggaact cccgcccagt
     6901 tgggaggtat gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg
     6961 cgtttttcta ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag
     7021 cgaaaatcgc caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg
     7081 atgccactaa atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg
     7141 ccattatcgc ccccgatgcc aatgccctgc ccgctgccgc acaagccgca gaaaacctga
     7201 aaaatgacaa agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg
     7261 aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag
     7321 tttatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata
     7381 ttcagggcgt aggtcaggtg gaagtctcgc caaacagcgt ccagggctat gactacgaag
     7441 cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg
     7501 gcaaatacga tttctgatgt gcattactta accggagtaa gttatggcag atttagacga
     7561 tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa aaaaatatcc cttttaccct
     7621 gaaaggttgc ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc
     7681 gaaaacgggt aaaaccgtga tgctggcttt tgaccatggt tattttcagg gaccaactac
     7741 cggacttgaa cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat
     7801 gtgtacgcgc gggattttgc gcagcgtagt tcccccggcg accaataagc cggtggtact
     7861 gcgggcgtca ggtgcgaact ctatcctggc ggaattaagc aatgaagccg tggcgttatc
     7921 gatggatgac gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag
     7981 cgaatatgaa catcagtcga tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt
     8041 gggaatgccg accatggccg tgactggcgt gggcaaagac atggtgcgcg atcagcgtta
     8101 tttctcgctc gcgactcgaa tcgccgctga aatggggacg caaattatca aaacctatta
     8161 tgtcgaaaaa ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg
     8221 cggtaaaaaa ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg
     8281 cgcttctggt gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat
     8341 gaaagccgta caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta
     8401 tctgagtgaa aaacagtaac tgcggattta aggagaagaa ttatgcacgt cacactggtt
     8461 gaaattaacg ttcatgaaga caaggttgac gagtttatcg aagtttttcg ccagaaccac
     8521 ctgggctctg tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccggaagtg
     8581 aattcgcgct tttatatcta cgaagcctat aaagatgaag acgcagtggc gttccataaa
     8641 accacgcccc actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt
     8701 aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat ttatcagttt tatctacaat
     8761 tggggtaacg cgctgacggg agtaaataaa tgtctgactg gaacccctct ttatatctgc
     8821 actttgccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga
     8881 atgtcgatta tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc ctgctgcatc
     8941 aacgttggcc tgctgccagg ataacgggca tcgactcgtc tccggcgatg attgctgaag
     9001 cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg
     9061 aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg
     9121 aattgtttcc tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc
     9181 cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact
     9241 acccggatcg cgggcgtgag tcgttggctg gtgttcatgc ttactacgat attttgagcg
     9301 aagccggatg tgaggtcgat atctggcgaa ccacctacta tcaccagatg ccgtcgcacc
     9361 aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg
     9421 agagcgaaca gcagcttttt cttacgcgct accatcagat gctgaaagag cagtatccac
     9481 tgcaagagaa cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta
     9541 cggagtaaat atcacgtcag ttggtaatgg cgatcgggaa gaacttttgc tggaatttcg
     9601 gcttcatcgt tcatctgtaa gaggtcaatt tcaatagcgt tacagatggc atccagcggt
     9661 aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa
     9721 ataaaagtat aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc
     9781 agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag
     9841 gcaaagggaa ttggcgtgta ggcaatgcgc tcgcagcctg ccagaaccgc tgaaatatcg
     9901 ttaagccgat cgttgaggct aataaacagg atatctgaaa gttgtccatt gcgtcgctga
     9961 accgccaacc actcccccat tattaacaag atgcggttag ctggagagtt cgaagccagt
    10021 acacgctgaa gatcttcagt cttgagataa tgagccagca cttccacctg tggctgtttg
    10081 cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca gccgggcaaa ctcccttaca
    10141 cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc
    10201 aactgccccc aaagtttgcg cgcttcaacg taacgggcgt aaccggcatt attacgaaaa
    10261 ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg
    10321 cccagatgcg tgtaccaggg cagcatgaaa ataacagcga tagaaaagag aaaattgagt
    10381 agtaagcgcg aggatatctt ggataatact gagccatgcc agacaaaaat acggcgcagc
    10441 cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta
    10501 aacgtgatta cgatcacatt ctcaagacac tacttacaaa ttacctacaa aattacaaca
    10561 ttaacaggtg ctgcatgtgg cacctgttaa tgataaaggc ttataggatg ttgtaactaa
    10621 tataatgaaa taagaaaccc ggtcgaaacc gggttcagag tattagcaca acggacgtac
    10681 agcttcgcgc atcccttttt cgagaatcgc atccaggtca ttagccacct gctcgaccag
    10741 accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac
    10801 cagttcctgg gtaccgataa cacggtcacg atgctgactc cagagttgct gataacgttc
    10861 cagccagtgt gcatcgtcct gtaccggata tgtttctcca ttacgctcac cgcgatagaa
    10921 cgcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg
    10981 ccctgccagc agctgcggca ggatgcgggt acggaacttg gtcataccgt tgagcgcgat
    11041 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
    11101 agattccagt tcatcgcgtg gcaaatccag taccggaata atttcttcgt agatggcttt
    11161 ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc
    11221 ctggaacgcc accggcacca acgcggtgtg tgcaccgttg agtattgcca ctttgcgttc
    11281 tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccagacgcag
    11341 ctcggtcgct aaggatttcg gtccctgaat cacaaacaag taaaagtgtt cagcggtgtc
    11401 gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat cacgcggata
    11461 accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg
    11521 aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc
    11581 gtcgccattg tagtcgatca actcacacgg gatgatgatc caacctttat ccagcgcacc
    11641 gttgaaatgg ctaaaacgtt cgaacagcag acgggtcagt tttgccggat aacttaccgc
    11701 tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga
    11761 gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt attcactgta
    11821 gacgctgatt tcacgattaa cagagcgaat cagacgcgcg tcgctgaccg cttccccctt
    11881 ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg
    11941 gaatgaagtt tcaatcggac gaacaacgac cacaccagaa ttcagatcgg tgtgctcatt
    12001 caggagatct atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat
    12061 gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg
    12121 ttcccttctg attagtcata caacctgttt gaattggtac gacatgttag caaactttaa
    12181 tacgacgaac ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag
    12241 tgtggcgcgg atcatggttt aatcgaggaa atacgccttt tcctggatca taaagtggta
    12301 gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact tcctcaccgt
    12361 aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga caggtatcgg
    12421 cttcgaccat caatttatta aagtcatcgg tcagcgttcg gtacgatgcg cgaccactac
    12481 cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt
    12541 caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc actgacggca
    12601 ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct
    12661 tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat
    12721 cgttaatact tttgaaatag tcgatatcaa gtagcatgac agtcagatgt tgtgtctgtt
    12781 tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa
    12841 aatcaaagtc ggctcgcagc gcaacttgct tcattagcga attgatcgct gccacgctaa
    12901 aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt
    12961 gcggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaattagcc accagtacga
    13021 tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg
    13081 cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg
    13141 aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg
    13201 gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga
    13261 attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg
    13321 aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc
    13381 gtaaggcact tactggctca tatttatttt tccccaaacg cttatcccgc acgaccagta
    13441 aggcgacggt aacaataaac atcatattgg agaaattgat agccagtgaa accagtcccc
    13501 attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca
    13561 gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga
    13621 aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta
    13681 gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaca cggaaagttg
    13741 aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa
    13801 cgtaataagt aaattctcat aaaggatagc atgttcgctg acagcggcgg caaataaagg
    13861 taccctggga atgctatttt gtcgttttcc gcaattctca tggctgattc atctgtacaa
    13921 ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt agcgaaacac
    13981 catgcgggat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct
    14041 gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg
    14101 ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt
    14161 tctccagaca atactgacgc tctttcatgc ccaaacgaaa gcgagatcga aattttgaac
    14221 gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg
    14281 tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc
    14341 cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac
    14401 gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc
    14461 actggtcgcc atcagcgcgt taatttgccg tgcctgcatt ggcgtcacca caggttcatc
    14521 aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca
    14581 gctcattttc agagcgcagt aatgaaaata gttttgcaga acggttgtca cgtcatgatg
    14641 gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa
    14701 ttcggaacgc gctaccaccg tatcgtagga aatatcagac acaccgctta agccgcgcac
    14761 gacttccagc atacgttgcc gtggtgcgct taatcgccct tgcaacatat cgcagaccac
    14821 cagcgcacca gcattaataa acggattacg cggaataccc tgctccattt ccagttgcac
    14881 taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc
    14941 ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa
    15001 acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatgcc
    15061 caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc
    15121 aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc
    15181 cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat
    15241 gtcgcgtcag atccggtctt tccacaccgt ctggatatta cagaattcgt gtaagccgaa
    15301 atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc
    15361 gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct
    15421 ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc
    15481 cagttccagc gcatgttctg catctttcgc aatggtgatt gccgcaacgg ggccaaacat
    15541 ttcttcccga aaagcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta
    15601 gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gcgccagggt
    15661 tttctccacc tgatgatgca gctcatcacg taaatcgaaa cgagccattg gtccgagagc
    15721 gttctcttcg tcacggggat cgcccatttt caaggctgcc gcagctgcca caaaacgttc
    15781 ggtaaatgcc gaagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg
    15841 tccggtattc tgataacgtc cggctaccgc cgctttaact gccagttcca gatcggcatc
    15901 gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc
    15961 tccagcctgt gcaccaatag ccgctccagc acgaacactt ccggtcaccg tgatagcagc
    16021 aatgcgcgag tctttaatca tctgagtgac accgtcattg tcggcattca gccagccata
    16081 tacgccttgt gggattcccg catctttaaa cacctgggca atgagctgag cacagcccat
    16141 cacattcggc gcatgtttaa gtaagtatcc gttaccagca agaatgatgg gaacagcgcc
    16201 acgcatcacc tgccataacg gaaaattcca cggcataatc gccagaatcg tccccaacgg
    16261 tcgatactca ataactgcct gctgattttc caccagcgta ggttccgcct tcagcattgc
    16321 cggaccatgt tctgcatacc agtcacacaa attcgccgat ttcgccactt cagcgcgcgc
    16381 ctggttgatt ggtttgccca tttcgcgggt gatcatttgc gccatttctt cgctacgggc
    16441 gcgcagagcc ttaccgatat cacgcagttt ttcagcacga taatctatat ttgtctcgcg
    16501 ccagtcacga aagcctgctg ccgccagctg aagtgcgttt tcgatatcat cagcgccagc
    16561 ccacggcaac acagaaagtt gttcacccgt ggcaggattt atcgaaattg catgagttgc
    16621 cggagtaatg gtcatcgggg tatctccttt atgagtcatg gtatgaagat acgcagattt
    16681 actcttgctt taaaatgaat aatattaagc cacttattca cgaatcgaga atattatgga
    16741 tctgacccaa ctggagatgt tcaacgcggt tgccgaagct ggcagcatta cgcaggctgc
    16801 agcaatagtg catcgcgtgc cgtcgaatct cactacccgt ctacgccagc tggaaacaga
    16861 actgggggta gatctgttta ttcgcgagaa tcagcgttta cgtctatctc ctgccgggca
    16921 taacttttta cgctatagcc aacaaattct cgcgttagtg gatgaagcgc ggagcgttgt
    16981 cgctggcgat gagccgcaag gtttattttc tctgggttcg ctggaaagca ccgcagcagt
    17041 tcgcattccg gccacgctgg cggaattcaa ccgtcgttat cccaaaattc agttttcact
    17101 ttccaccggc ccttccggca ctatgctgga aggtgtgctg gagggaaaac ttaatgcggc
    17161 gtttattgat ggacccatta accatactgc cattgacggg ataccggtat accgcgagga
    17221 gctgatgatc gtcacgccac aaggtcatgc gccagtaatc cgtgccagtc aggttaatgg
    17281 cagtaacatt tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg agagctggtt
    17341 tcatgctgac ggtgccgctc cgggaactat ccatgagatg gagtcttatc acggaatgtt
    17401 ggcctgtgtg gtcgcaggag caggtattgc gcttattccg cgctctatgc tggaaagtat
    17461 gccggggcat caccaggttg aagcgtggcc gttagctgag caatggtgtt ggctaacaac
    17521 ctggctggtc tggcgtcgtg gtgcgaaaac gcgtccgctc gaggcattta ttcaactact
    17581 ggatgtgcct gactcgacaa aacagggtta ccaatgagct atttttgata gttcttgcgg
    17641 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
    17701 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
    17761 gcaccgcagg taaaagaagt tttaaaggat tttattgatg cacttagtac aataatatgt
    17821 aatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataat
    17881 cttgacaact tatcatatat tcagaggact tcttttcggg gtaacgacac gctatacaat
    17941 gaaaaggttc agtttaaact cacttatccc gccaggaatg gaagacacaa agaaaatatt
    18001 gaatttcagg tagtaataaa tttaagccct atttatttag ataatttccg ccatgatgga
    18061 gaaattaata ttttttgcgc tcccaaccca aagcctgtta ctatggggcg cgtatttcag
    18121 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca attcccaatg
    18181 atcaaccctg gcgttcccat caaaagagcg catacaccac atattgaccc cctgcctccg
    18241 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
    18301 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
    18361 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
    18421 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
    18481 taagtctgca tctgtttttt atccctttta gcagaacaag aatctcctca ttttcaggac
    18541 ttgtttttat ttttcgccaa tatgttgtta cactatcacc aatttatgaa tcccaatgaa
    18601 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaaacat
    18661 atggcgcgta agaccctcaa actgtttgta aaaaatgaaa caccgccaaa agttactcat
    18721 gaagatctgg tgaaaaacag gcctcgctcc cctgtcagac cgcctatacc cgcaacctcc
    18781 aaaacgccag acattcctga tcgtcattaa accgtgatgt ttaccatctc tctgacgcgt
    18841 gaaagaatca gcgtcagaga aacggaaaac gcgatccaga tcacaaatgc attgtattca
    18901 catcattaac cgttttaaga tcatttcatc actttttcgc aactcacccg ataatctgtt
    18961 atgacaacaa acactgtttc ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc
    19021 gccgccttca tcttcaacac caccgaattt gtccctgttg gcctgctctc tgacattgcg
    19081 caaagttttc acatgcaaac cgctcaggtc ggcatcatgt tgaccattta cgcatgggta
    19141 gtagcgctaa tgtcattgcc ttttatgtta atgaccagcc aggttgaacg gcgcaaatta
    19201 ctgatctgcc tgtttgtggt gtttattgcc agccacgtac tgtcgttttt atcgtggagc
    19261 tttaccgttc tggtgatcag tcgcattggt gtggcttttg cacatgcgat tttctggtcg
    19321 attacggcgt ccctggcgat ccgtatggct ccggccggga agcgagcaca ggcattgagt
    19381 ttaattgcca caggtacagc actggcaatg gtattaggat tacctctcgg gcgcattgtg
    19441 gggcagtatt tcggttggcg aatgaccttc ttcgcgattg gtatcggggc gcttattacc
    19501 cttttgtgcc tgattaagtt acttccctta ctgcccagtg agcattccgg ttcattgaaa
    19561 agcctcccgc tattattccg ccgcccggca ttgatgagca tttatttgtt aactgtggtg
    19621 gttgtcaccg cccattacac ggcatacagc tatattgagc cttttgtgca aaacattgcg
    19681 ggattcagcg ccaactttgc cacggcatta ctgttattac tcggtggtgc gggcattatt
    19741 ggcagcgtga ttttcggtaa actgggtaat caatatgcgt ctgcgttggt aagtacggcg
    19801 attgcgctgt tgctggtgtg cctggcactg ctgctacctg cggcgaacag tgaaatacac
    19861 ctcggggtgc tgagtatttt ctgggggatc gcgatgatga tcatcgggct tggtatgcag
    19921 gttaaagtgc tggcgctggc accagatgcc accgacgtcg cgatggcgct attctccggg
    19981 atatttaata ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag tctgcactgg
    20041 tcaatgtcga tgattggtta tgtgggcacg gtgcctgctt ttgccgcgtt aatatggtca
    20101 atcattatat ttcgccgctg gccagtgaca ctcgaagaac agacgcaata gttgaaaggc
    20161 ccattcgggc cttttttaat gattcgtttt aatgatttcc aggatgccgt taataataaa
    20221 ctgtacaccc atacatacca gcaggaaccc catcaagcgg gagatcgctt caatgccacc
    20281 cttgcccacc agccgcataa ttgcgccaga gctgcgtaga cttccccaca aaataaccgc
    20341 caccaggaaa aagatcagcg gcggcgcaac catcagtacc caatcagcga aggttgaact
    20401 ctgacgcact gtggacgccg agctaataat catcgcaatg gttcccggac cggcggtact
    20461 tggcattgcc agtggtacaa aagcaatatt ggcgctgggt tcatcttcca gctcttccga
    20521 cttgcttttc gcctccggtg aatcaatcgc tttctgttgc ggaaaaagca tccgaaaacc
    20581 gataaacgcg acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt
    20641 atccatcacc agttgcccgg cgtaatacgc caccatcatg atggcaaaga cgtacaccga
    20701 ggccatcaac gactgacgat tacgttcggc accgctcatg ttgcctgcca ggccaagaaa
    20761 taacgcgaca gttgttaatg ggttagctaa cggcagtaac accaccagcc ccaggccaat
    20821 tgctttaaac aaatctaaca ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa
    20881 agtataaggg gtaaacaagg ataaagtgtc actctttagc taaccttgca tcgcattgac
    20941 caaaacttga accgatttag caaaacgtgg catcggtcaa ttcattcatt tgacttatac
    21001 ttgcctgggc aatattatcc cctgcaacta attacttgcc agggcaacta atgtgaaaag
    21061 taccagcgat ctgttcaatg aaattattcc attgggtcgc ttaatccata tggttaatca
    21121 gaagaaagat cgtctgctta acgagtatct gtctccgctg gatattaccg cggcacagtt
    21181 taaggtgctc tgctctatcc gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt
    21241 gttgtcggtc gacctgggag cactgacccg tatgctggat cgcctggtct gtaaaggctg
    21301 ggtagaaagg ttgccgaacc cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag
    21361 cggcgcggca atatgtgaac aatgccatca attagttggc caggacctgc atcaagaatt
    21421 aacaaaaaac ctgacggcgg acgaagtggc aacacttgag catttgctta agaaagtcct
    21481 gccgtaaaca aaaaagaggt atgacgatgt ccagacgcaa tactgacgct attaccattc
    21541 atagcatttt ggactggatc gaggacaacc tggaatcacc actgtcactg gagaaagtgt
    21601 cagagcgttc gggttactct aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc
    21661 attcattagg ccaatacatc cgcagccgta agatgacgga aatcgcgcaa aagctgaagg
    21721 aaagtaacga gccgatactc tatctggcag aacgatatgg cttcgagtcg caacaaactc
    21781 tgacccgaac cttcaaaaat tactttgatg ttccgccgca taaataccgg atgaccaata
    21841 tgcagggcga atcgcgcttt ttacatccat taaaccatta caacagctag ttgaaaacgt
    21901 gacaacgtca ctgaggcaat tatgaaaccg cttttatccg caatagcagc tgcgcttatt
    21961 ctcttttccg cgcagggcgt tgcggaacaa acccagcaac cgctcgttac ttcctgtggc
    22021 gatgtggtgg ttgttccccc atcgcaggaa caaccaccgt tcgatttaaa tcacatgggt
    22081 acaggcagtg acaaatcgga tgcgctgggc gtgccctatt ataaccaaca agccatgtag
    22141 tatattcagg ccccggcata tcggggcttg ttaacctctt accttcaccg ctttacgcca
    22201 ctgcaaacca aatacattga tatacagtcc ggccataatg agcacagccc ctaagaattg
    22261 cagacctgtt aagcgttcat ccagcagtaa cgctgcactt gccagaccga ctaccggcac
    22321 cagtaatgat aatggtgcga cacgccatgt ttcatagcgt ccaagcagcg taccccaaat
    22381 cccgtagcca actatcgtcg ctacaaatgc cagatacatc aacgccagaa tggtggtcat
    22441 gtcgatagtg accagactgt gaatcatttt tccggagcca tcgagaatga gcgaggcgac
    22501 aaaaaacgga attatcggga ttaatgcgct ccagacgacc agagacatta ccgccggacg
    22561 ggatgaatga gacatgatct ttttgttgaa gatattgcca ctcgcccaac taaatgccgc
    22621 tgccagagtc aacataaagc cgagcatcgc cacatgctga ccgttaaagc tatcttcgat
    22681 taacaccagt acgccagaaa tcgctaacgc aatccccgcc agttgcttac cgtgcagccg
    22741 ttcgccaaaa gtaaacgcgc caagcacgat agtaaaaaac gcctgagcct gtaagaccag
    22801 cgaggccagt ccagccggca taccgaagtt aatggcgcag aagagaaagg caaactgtgc
    22861 aaaactgatg gttaaaccat accccagcag taaattcagc ggtacttttg gtcgtgccac
    22921 gaaaaagatt gccgggaagg cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg
    22981 catgttatga agccccactt tgatgaccac aaaattaagc ccccatacaa cgacgactag
    23041 tagcgccaac accccatctt ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa
    23101 gcaatcaact tagctgaatt tacttttctt taacagttga ttcgttagtc gccggttacg
    23161 acggcattaa tgcgcaaata agtcgctata cttcggattt ttgccatgct atttctttac
    23221 atctctaaaa caaaacataa cgaaacgcac tgccggacag acaaatgaac ttatccctac
    23281 gacgctctac cagcgccctt cttgcctcgt cgttgttatt aaccatcgga cgcggcgcca
    23341 cgctgccatt tatgaccatt tacttgagtc gccagtacag cctgagtgtc gatctaatcg
    23401 gttatgcgat gacaattgcg ctcactattg gcgtcgtttt tagcctcggt tttggtatcc
    23461 tggcggataa gttcgacaag aaacgctata tgttactggc aattaccgcc ttcgccagcg
    23521 gttttattgc cattccttta gtgaataacg tgacgctggt ggtgctcttt tttgccctca
    23581 ttaactgcgc ctattccgtt tttgctaccg tgctgaaagc ctggtttgcc gacaatcttt
    23641 catccaccag caaaacgaag atcttctcga tcaactacac catgctcaat attggctgga
    23701 ccgtcggccc gccactcggc acgctgttgg tcatgcagag catcaatctg cccttttggc
    23761 tggccgctat ctgctctgcg tttcccatgt ttttcattca aatttgggta aagcgcagcg
    23821 agaaaatcat cgccacggaa acaggcagtg tctggtcgcc gaaagtttta ttacaagata
    23881 aggcactgtt gtggtttacc tgctctggct ttctggcttc ttttgttagt ggcgcatttg
    23941 cttcatgcat ttcacaatat gtgatggtga ttgctgatgg tgattttgcc gaaaaggtgg
    24001 tcgcggttgt tcttccagtg aatgctgcca tggtggttac gttgcaatat tccgtgggcc
    24061 gccgccttaa cccggctaac atccgcgcgc tgatgacagc aggcaccctc tgtttcgtca
    24121 tcggtctgat cggttttatt ttttccggca acagcctgct attgtggggt atgtcagctg
    24181 cggtatttac tgtcggtgaa ataatttatg cgccgggcga gtatatgttg attgaccata
    24241 ttgcgccgcc aggaatgaaa gccagctatt tttccgccca gtcattaggc tggttgggcg
    24301 cagcggtaaa tccattagtg agtggtgttg tacttaccac tctcccgtcg tcttcgctgt
    24361 ttatcattct ggcgttagtc atcattgccg cgtgggcgct gatgttaaaa ggtattaacg
    24421 ccagacccca gaggcaacca gcactctgtt gacgttcgtg ccgttccact actggcacgg
    24481 cttcgctaat cattattctg aggcaaaggc atagccttca tcttcccagc cggttatccc
    24541 gccgagcatg attttaaccg gctttccgag tcgagccagt ttgagtgccg cacggtctgc
    24601 accgttacaa tgtggccccg cgcaatacac cacaaatagc gtatcgtcgg gccattcggc
    24661 cattctttct gcggtcattt gcttatgggg taaatggaga gcgcctggga tatgccgacg
    24721 cgcgaagtcc tcttcacgtc caaccacatg aagtaaaacg aaatcatcgc caatataagc
    24781 cagagagtca tgaacatcgg cgcaatctgt ttcgacactc aagcgcgcga gaaaatgcgc
    24841 ctcagcaact tctggcttcg ctgtggtaaa ttcagtgaca taacgcatat tgctttgtcc
    24901 ttttggtttg ttgtgttaac aataatgtaa ctaatctacc tttcactgac cgcctctcga
    24961 tatgccagaa atgagcaaaa tgatgacaac gttacaacgt ccgctggtca ttgcactggc
    25021 atatgacggg ctatgtactt ttgaatttgg cgttgccgtt gaaatattcg gcctgccacg
    25081 accggaactg ggcgataact ggtatcgttt tgctgttgct gcggtagaac caggtgcgct
    25141 acaagccacc ggggggatcc acgtggttgc tgatggtgga ctggagttat tgcccaacgc
    25201 cgatatgatt attattcctg gctggcgcgg aatagatgcc aaagtgccgg agatgctatg
    25261 ccagtcattg cgcgaggcac accagcgcgg tactcgcctg gtatcaatat gctctggcgt
    25321 gtttgtcctt gctgctgcgg gccttcttca tcagcgaaaa gcaaccaccc actggcgcta
    25381 cactgaacaa ctccagcacc gttacccttc aatcagcgta agtgacaatg tgttatatca
    25441 ggatgaagga caagtgatga cctccgccgg aagcgccgcg gggatcgatc tgtgtttaca
    25501 tatcgtgcgc aaagattttg gtcatgagat tgctaataat gtcgcccggc gactggtcat
    25561 tcagccacat cgccaggggg atcaaccaca aaatctgacg cgcccaatgg cctcaccgcg
    25621 tgaaagccag acgttaggtg ccttattcga ctttttgcag cagaatctgg cacaaacaca
    25681 tactgtgtct tccctcgccg aacgggtaaa tatgagtccg cgcacttttt taagacgttt
    25741 tgaggcgtta actggcatga cgcccgcccg ctggctgctc aatgagcgct taatacgagt
    25801 acaggattat ctgcaaaatt cggtttttaa catcgagaaa attgccgagc tgacgggttt
    25861 tggcaattca gcgttattgc gtcatcactt tcaccaacgt tttttattat ctccttcgca
    25921 atatcgcaaa aacagccgtt ccccatcaca ataatcgcct gattcagcct ttgtttagat
    25981 aacctctgct aatatctttc tcattgagat aaaaattaag gtaagcgagg aaacacacca
    26041 caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc catactggga
    26101 ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga tgactaatga
    26161 acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag gggcctgatt
    26221 tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca tggatagatt
    26281 tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc gttgacacag
    26341 tagcatcagt tttctcaatg aatgttaaac ggagcttaaa ctcggttaat cacattttgt
    26401 tcgtcaataa acatgcagcg atttcttccg gtttgcttac cctcatacat tgcccggtct
    26461 gctcttccaa tgaccacatc cagagtctct tcaggaaatg cgcgactcac acctgctgtc
    26521 acggtaatgt tgatatgccc ttcagaatgt gtgatggcat ggttatcgac taactggcaa
    26581 attctgacac ctgcacgaca tgcttcttca tcattagccg ctttgacaat aatgataaat
    26641 tcttcgcccc cgtagcgata aaccgtttcg taatcacgcg tccaactggc taagtaagtt
    26701 gccagggtgc gtaatactac atcgccgatt aaatgcccgt aggtatcatt aaccaattta
    26761 aatcggtcaa tatccaacaa cattaaataa agattcagag gctcagtgtt gcgtaactga
    26821 tgatcaaagg attcatcaag aacccgacgc cccggcaatc ccgtcaaaac atccatattg
    26881 ctacggatcg tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa agaaagcacc
    26941 ccctcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat agccagcatt
    27001 aattcccgac cacaattatg catatgttga tgggcagaat ccattagccg aacgtaaggt
    27061 aattcatcgt tatcgagtgg cctcagatga tcaatccacc gaccaaactg gcacagtcca
    27121 taagaatggt tatccgttat ttctggctta ctggcatctc tcgcgaccac gctgtgaaac
    27181 atactcacca gccactgata gtgggcatcg atagccttat tgagatttaa caagatggca
    27241 tcaatttccg ttgtcttctt gatcattgcc actccttttt cacagttcct tgtgcgcgct
    27301 attctaacga gagaaaagca aaattacgtc aatattttca tagaaatccg aagttatgat
    27361 tcatctctga gataacattg tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt
    27421 ctgtaaatta cctcttcatt atcgcaaatg aaaggatgac aaatagcata acccagtacc
    27481 caaatggccc ggtagttcag gccatcaggc taatttattt ttatttctgc aaatgagtga
    27541 cccgaacgac ggccggcgcg cttttcttat ccagactgcc gctaatgttg atcatctggt
    27601 ccggctgaac ttctcgtcca tcaaaaacgg tcgcaggaat aacaacatta atttcaccac
    27661 tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg
    27721 aaaccgaagc gccatcgtgc atggtttttg cgaaatcaac agtcattttt tttgcatcat
    27781 cggttccgcg atagccatct tctattgcat gaggcggcgg tggcgctgca tcctgtttta
    27841 aaccgccctg gtcatctgcc aacgcataag gtatgacaag aaaacttgct aatacaatgg
    27901 cctgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
    27961 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagcca
    28021 actagctgtc aaatattcag agaatttaac tca
//
LOCUS       DAZGXS010000054        27823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGXS010000054 DAZGXS010000000
VERSION     DAZGXS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            83..745
                     /gene="acrS"
                     /locus_tag="ACHKG5_002422"
     CDS             83..745
                     /gene="acrS"
                     /locus_tag="ACHKG5_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0868516.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(748..927)
                     /locus_tag="ACHKG5_002423"
     CDS             complement(748..927)
                     /locus_tag="ACHKG5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0868517.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1011..1895)
                     /gene="yhdJ"
                     /locus_tag="ACHKG5_002424"
     CDS             complement(1011..1895)
                     /gene="yhdJ"
                     /locus_tag="ACHKG5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0868518.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHKG5_002425"
     CDS             complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHKG5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0868519.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHKG5_002426"
     CDS             complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHKG5_002426"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0868520.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHKG5_002427"
     CDS             complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHKG5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0868521.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHKG5_002428"
     CDS             complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHKG5_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0868522.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5930..6172)
                     /locus_tag="ACHKG5_002429"
     CDS             complement(5930..6172)
                     /locus_tag="ACHKG5_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0868523.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHKG5_002430"
     CDS             complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHKG5_002430"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0868524.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHKG5_002431"
     CDS             complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHKG5_002431"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0868525.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9089..10063)
                     /locus_tag="ACHKG5_002432"
     CDS             complement(9089..10063)
                     /locus_tag="ACHKG5_002432"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0868526.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10215..12155
                     /gene="csrD"
                     /locus_tag="ACHKG5_002433"
     CDS             10215..12155
                     /gene="csrD"
                     /locus_tag="ACHKG5_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0868527.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12460..13503
                     /gene="mreB"
                     /locus_tag="ACHKG5_002434"
     CDS             12460..13503
                     /gene="mreB"
                     /locus_tag="ACHKG5_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0868528.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13569..14672
                     /gene="mreC"
                     /locus_tag="ACHKG5_002435"
     CDS             13569..14672
                     /gene="mreC"
                     /locus_tag="ACHKG5_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0868529.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14672..15160
                     /gene="mreD"
                     /locus_tag="ACHKG5_002436"
     CDS             14672..15160
                     /gene="mreD"
                     /locus_tag="ACHKG5_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0868530.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHKG5_002437"
     CDS             15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHKG5_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0868531.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15752..17221
                     /gene="rng"
                     /locus_tag="ACHKG5_002438"
     CDS             15752..17221
                     /gene="rng"
                     /locus_tag="ACHKG5_002438"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0868532.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHKG5_002439"
     CDS             17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHKG5_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0868533.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVVVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21245..22690
                     /gene="tldD"
                     /locus_tag="ACHKG5_002440"
     CDS             21245..22690
                     /gene="tldD"
                     /locus_tag="ACHKG5_002440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0868534.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22824..23753)
                     /gene="aaeR"
                     /locus_tag="ACHKG5_002441"
     CDS             complement(22824..23753)
                     /gene="aaeR"
                     /locus_tag="ACHKG5_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0868535.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23936..24139
                     /gene="aaeX"
                     /locus_tag="ACHKG5_002442"
     CDS             23936..24139
                     /gene="aaeX"
                     /locus_tag="ACHKG5_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0868536.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24147..25076
                     /gene="aaeA"
                     /locus_tag="ACHKG5_002443"
     CDS             24147..25076
                     /gene="aaeA"
                     /locus_tag="ACHKG5_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0868537.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25082..27049
                     /gene="aaeB"
                     /locus_tag="ACHKG5_002444"
     CDS             25082..27049
                     /gene="aaeB"
                     /locus_tag="ACHKG5_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0868538.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27141..27413
                     /locus_tag="ACHKG5_002445"
     CDS             27141..27413
                     /locus_tag="ACHKG5_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0868539.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27469..27732)
                     /gene="yhcN"
                     /locus_tag="ACHKG5_002446"
     CDS             complement(27469..27732)
                     /gene="yhcN"
                     /locus_tag="ACHKG5_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0868540.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6678 a         6955 c         7413 g         6777 t
ORIGIN      
        1 ataaatacac attaggtgat agattaacct tcgctatttt ctcactctgt gtcgaagata
       61 tttatttcct gaataattaa tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg
      121 gcaagaactg attgaaactg ccatcgccca gtttgcgcaa catggcgtaa gcaagacgac
      181 gctcaacgac attgccgatg ccgctaacgt tacgcgtggc gctatctact ggcacttcga
      241 gaacaagacc caactgttta atgagatgtg gttgcaacag ccttcattgc gggagttaat
      301 ccaggaacac ttgacggctg gattagagca tgacccgttt caacaattgc gtgaaaaatt
      361 gattgtcggc ttgcaatata ttgccaaaat tccccgccag caggcgttgc tgaaaatctt
      421 atatcacaaa tgtgaattta atgatgagat gctggccgag ggagtgatac gcgaaaagat
      481 gggctttaat ccgcagactc tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt
      541 agcaaataac ctcgatttag atgttgtgat gattattatt gatggtgcct tcagcggaat
      601 tgttcaaaac tggttaatga atatggcggg ttatgatctt tataaacaag cccccgctct
      661 ggtcgataac gtattaagaa tgttcatgcc agatgaaaac ataacgaaat taattcatca
      721 aacgaatgaa ttaagtgtca tgtaaattca cattctgtcc tgaaaattca gttcgtcaca
      781 gttaaccagt tttagtggat taactgagtt catattgcgg catttgtctg tttctgttgc
      841 cagcagttca atccactgca tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac
      901 gacgagccac caatacttgc gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg
      961 caggaaatat acacgaaatg aataatctgc aaaccaggct tacacatcct ttactttgta
     1021 atgagatcgg ggtcaacttc gcttaaccgg cttcgttttg acaggttgcc ggtctttctt
     1081 tttttcactt tcgccagttc ttccgcagag taatgcgacg cgacatccag ccgtcgaagc
     1141 cccattttga tgtactcgct gttgatctca ataccaatga attttcgtcc gctggcgatg
     1201 gctacggcac cggtagtaaa gctaccagca aacgggtcga gaacgatatc gcctgggttg
     1261 gaagaggcga gaataatgcg tttcagtaag gcttccggtt tttgcgtcgg gtggttttca
     1321 tattcatcca tcaaataacg cacgcgcgga aaatcccaga cgttacccgg tactttttga
     1381 tgattgtaag gctgtggagg atttttgcga taatcgatca acgcgcgctg cgatccggtt
     1441 ttggcttcta ccagaatagc atcaccgttg aatgtgtagt tctttgcgtc tttcaccatc
     1501 atcaggatgg gttcgtacat ggagccgtag tgttttttcg cctgcactcc agaactgtca
     1561 tatgaccaga cgatgcgact tttgatggta aaaagcttgc ggcactggag atcgataaag
     1621 ggcatgtttt ccgtactgtt catgatgtac atgctgccct gctttttcag aacgcggtgg
     1681 cactctgcaa tcacttctaa cagccagtcg ataaacagat cttctttcca ggcttcgatc
     1741 agaccatcaa aatttttacc gatgttatac ggtgggtcgg caaagatcag atcgacgctt
     1801 tcagtaggta gctttttaag ttcggcaagg gcatcaccgt gaataatggt cttagcttca
     1861 ttaccaaacc gggtcggttc acatcctgtt ctcatggtca ctccctttgt gacacctata
     1921 aaaaaggcgc ttccccatgc cgagtagcgc cttttaatca agcaattagc taacctgaat
     1981 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     2041 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     2101 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     2161 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     2221 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     2281 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     2341 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     2401 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     2461 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     2521 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     2581 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     2641 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     2701 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     2761 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     2821 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     2881 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     2941 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     3001 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     3061 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     3121 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     3181 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     3241 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     3301 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     3361 gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca taaaaatcat
     3421 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     3481 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataatcggca aatttacgtg
     3541 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct atggcgatgc gaaggttaat
     3601 tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc agagcgaagc
     3661 tgtcggcata ggcttcgcaa acgctctccg cctggcttgc cagaatacca gaaaggccca
     3721 gtaagccgcc tgaaactggc aggacgctaa ttaacggtgc cagttcacgt aatgggcctg
     3781 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     3841 agagttccag acggtcagaa acgccattac gttcggcgtt atcgcggctg gcctgaatcg
     3901 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg
     3961 ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt accggttaaa tcgaggctgt
     4021 cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac gccagccctg
     4081 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     4141 acagtcgttc gccaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     4201 tatcttctag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     4261 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     4321 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     4381 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tccgcgttcg
     4441 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     4501 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     4561 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     4621 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     4681 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     4741 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     4801 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     4861 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag
     4921 caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     4981 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     5041 atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc
     5101 gactacaatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt tataagagat
     5161 acagcgcacc gcagtatgcg gcaggcctat aacgccaaag cacaccagta cccagaacga
     5221 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     5281 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     5341 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     5401 ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa tcagcagccc
     5461 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     5521 ggtcatcgca ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     5581 actctggtag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag
     5641 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgccggaa gctgaatcat
     5701 cgccagcaat acccagccaa ggccgtattt gtaagcagct ccaggcccgc cgataaacga
     5761 actggcactg atataagtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
     5821 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     5881 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
     5941 cgtcatcctc cagcgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
     6001 taaacagcag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
     6061 cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca
     6121 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
     6181 cctgatgggt gaagaaagcg aggattgtac cttatgggtg ttgtcgatcc ccagtaataa
     6241 aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga
     6301 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     6361 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     6421 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     6481 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
     6541 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcccgg acttggcagg aaggtgttcg
     6601 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     6661 tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg tcaacgccgg
     6721 tgatcatttc tgtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     6781 actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg atatcaacac
     6841 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     6901 gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     6961 agatagagtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
     7021 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     7081 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
     7141 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
     7201 tatccatatc gtcgcccagc gggccgtcag aacccggtac gcaagggacg cccgcttttt
     7261 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     7321 caatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     7381 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
     7441 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     7501 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
     7561 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     7621 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     7681 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     7741 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     7801 tcgatgaacg cttttgcatc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
     7861 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     7921 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
     7981 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
     8041 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     8101 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     8161 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggtc
     8221 gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac attgtgcagc
     8281 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     8341 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     8401 caattggcag ctaaatactg gtcttatcag ggaagataat caacaactaa catgtaaata
     8461 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     8521 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
     8581 gctgcaatga gataatcgtc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     8641 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
     8701 tagatgtgaa tgctaaagca cgcaaaatta accgattgac aatgctcagc attaaatagg
     8761 ttcgagtgat taatttgtag tcaaacaaaa tcagattatt cacctcatgg taccagaaat
     8821 aagtaagggg ttaagcgtag cgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
     8881 aggcgtatca ataatagctg tttagtggag tatcaatgcg catcaattta tggtcagtcg
     8941 aaatcatgac tttccagttt ggatgatgtt gtacaacttc atgaccaagg tgttcgacga
     9001 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatttc aaaatgagtt
     9061 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
     9121 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
     9181 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
     9241 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
     9301 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
     9361 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
     9421 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
     9481 tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
     9541 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
     9601 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
     9661 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
     9721 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
     9781 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
     9841 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
     9901 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
     9961 gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg
    10021 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    10081 atgatggatt ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat
    10141 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    10201 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    10261 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gccattcagt
    10321 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    10381 atgacttcag cgcattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    10441 gtgtagacct gctccatggt gataagcagg tttataccct ggccagaaat ggtagttatc
    10501 gtccggttgg caccagcgat ctgtttcgtg aactgagcgt tccgttgata aagcatccgg
    10561 ggatgtcgtt gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tcgttgatga
    10621 ccaccgcgcc gctcacaggg gcgattggct ttatcattct tatgctcttt ctggcggtac
    10681 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    10741 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    10801 gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    10861 ttgatacgct gatccgctct tatgccgcac aggacgtgaa aaccggcctc aataaccgac
    10921 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    10981 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatacc tgggggcaca
    11041 gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct
    11101 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    11161 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    11221 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    11281 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    11341 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    11401 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    11461 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    11521 ttgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    11581 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    11641 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    11701 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    11761 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    11821 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    11881 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    11941 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctcaggga
    12001 ccagcaccca ggtttacgcc accggtgtgc gttcgcgaag cgagtggcag accctgattc
    12061 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    12121 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    12181 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    12241 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    12301 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    12361 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    12421 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    12481 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    12541 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    12601 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    12661 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    12721 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    12781 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    12841 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    12901 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    12961 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgta
    13021 cgcattggtg gtgaccgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    13081 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttatccg
    13141 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    13201 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    13261 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    13321 gagcgcggca tggtactcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    13381 atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc
    13441 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    13501 taatcggatg caggcagggg aagcgtctgt ttaccctgcc tggtctgata cgagaatacg
    13561 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    13621 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    13681 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    13741 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaatc
    13801 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    13861 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    13921 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    13981 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    14041 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    14101 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    14161 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    14221 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    14281 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    14341 cagggctgca acgtttgcgt tatctgctgc tgctgtgggg ggcagatcgt aacggcgcta
    14401 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    14461 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    14521 tcgctcagcc gactccgcag caacccacga caggaaatgc agctactgcg cctgctgcgc
    14581 ccacacagcc cgctgctaat cgctctctac aaagggctac gccgccgcaa agtggtgctc
    14641 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgctg
    14701 ggtaatctgg ctctctttcc tcattgcgct tttgctgcaa atcatgccct ggccggataa
    14761 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    14821 gccccatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    14881 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    14941 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    15001 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    15061 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    15121 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    15181 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt
    15241 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    15301 gttgtgcgtc tggcgcgcga gaaagcacgg gcaggtgtcg cgcaaacggc gaaggatctc
    15361 ccggtgctgg gtgcggatac tatcgttatc ctgaacggag aagtgctgga gaaaccgcgc
    15421 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    15481 acggcagtgg cgttggccga cagccggcac attctcgatt gcctggtggt caccgatgtg
    15541 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    15601 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    15661 ggcagctatc aagccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat
    15721 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    15781 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    15841 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    15901 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    15961 atctgacatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    16021 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    16081 tccgcttggc actaaggggg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    16141 ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa cgaattgaaa gcgaatcaga
    16201 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    16261 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    16321 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    16381 cggcgaactg gcgttggcgc agcgtgttct gcgtgatttc gccgatgccg aactggaccg
    16441 cattcgcgtt gactcacgcc tgacttacga agcgttactt gagttcacct cggagtacat
    16501 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    16561 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gttgaactga aatccggtgg
    16621 ttatctgatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    16681 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    16741 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    16801 tatgaataat gaagatcacc gccgccgagt gctgcactcg ctggagcagg cgttgagcaa
    16861 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    16921 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    16981 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    17041 tgtacaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    17101 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    17161 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    17221 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    17281 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    17341 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    17401 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    17461 cagccagctg gcagaatttt ggcccgacgc ttgaagcgca cgacatccgt gcagaactaa
    17521 aggatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    17581 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    17641 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    17701 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc
    17761 catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    17821 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    17881 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctcc
    17941 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgcgc
    18001 tggaaacggc gcagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    18061 gcggtgacgt ctggctgaaa cagggtggtg ccagctggtt gggcgagaag gaaacgcata
    18121 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccggga tggcagttct
    18181 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    18241 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    18301 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    18361 aactttcacc tgcactgggt gatgtttggc gctctacaca accgagcggc aagattaaca
    18421 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    18481 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    18541 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    18601 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    18661 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    18721 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    18781 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact
    18841 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    18901 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    18961 agtttgaagt ggtggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    19021 cattaactaa ccttggtatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    19081 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    19141 caaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    19201 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    19261 acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgag ctggtaaccg
    19321 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    19381 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    19441 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    19501 cctaccaggt cgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    19561 ctgcagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    19621 ttctgcctta tcatgctggc gcgacgtata acgtagagct aaacggcgat ttgaagaatg
    19681 tgagcagtca cttaccttca ccgttagcca aacctgcggg tgaaccactg ccggtaaacg
    19741 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataatc
    19801 atttcaatag ccgctggttg ctcggtcaaa agctgacgct cgaccgtgct atttgggcgg
    19861 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg tgttgaactc aatatgccgc
    19921 cgatgaatgg tgccgagtgg ctggccctgt ttcagaaagg tgcggcggag agtgtcggtg
    19981 gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtcg ctgggaaatc
    20041 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc caggttgagg
    20101 cgcaggggcg tgaaatcaac gccacgctgg cgatgcgtaa taacgcgccg tggctggcga
    20161 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    20221 catcaccttt cccgacaacg gaacgcatta acttccgtgg ctggtcggac gcccaaatac
    20281 gatgcgcaga gtgctggttc tgggggcaaa aattcgggcg cattgacagt gatatcacca
    20341 tttctggcaa tacattaacg ctgaccaatg gactgattga tactggtttt tcgcggctca
    20401 ctgccgatgg tgaatggatt aacaatccgg ggaatgaacg tacctcgctg aaaggaaaac
    20461 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacggc
    20521 agtcgtcatt taatgtggat tacgatttac actggcgtaa agcaccgtgg cagccagatg
    20581 aggcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    20641 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    20701 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    20761 ccgcgtggat taaagacggc gttatgcaca ccgacgacac gctggtggat ggcctggagg
    20821 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    20881 ttgtcgcacc agagatttct gcgacagtgg gcgtggctgc ggcttttgcg gttaacccca
    20941 ttgttggcgc ggcagtgttt gccgccagta aagtgctggg gccgctgtgg agcaaagtct
    21001 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    21061 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    21121 ataggataat cgttgccaaa ggccaacgag ccagaacata accgtaggct ggatagggcg
    21181 ttcacgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    21241 aacgatgagt cttaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca
    21301 ggacttgttc gtgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    21361 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    21421 ttacaacatc gatcagggcg ttggtgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    21481 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    21541 cgcccgtgat agcggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    21601 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    21661 tcgcgtcgat aaggttgccc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    21721 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    21781 ccgtccgctg gtgcgtcttt ccgtgagcgt tctgatcgaa gaagatggca aacgcgaacg
    21841 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atctcgacgg
    21901 cgaagtccgt gcggatgcat gggcaaaaga agcagtacgt atggcgctgg tcaatctttc
    21961 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    22021 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gtggcacttc
    22081 agtatttagt ggacaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    22141 cggtacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    22201 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    22261 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    22321 catgccgcgt atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    22381 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    22441 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    22501 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    22561 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    22621 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    22681 tactgcgtaa taatttcagt attttcagaa ggataattaa tccttcctcg tgcacgaacg
    22741 gtcccctcgc ccctttgggg agagggttag ggtgagggga acccgttggc acaggtttgt
    22801 acaacgtaac agtacaatat gaattacttc tctttcccgc gcccgtgcat ctcctgaaac
    22861 aatttaccga cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc
    22921 ggcagcttat ctttttcggt atataacgca taaaccgggc gtggatctga ctgataacgc
    22981 ggcagcagga tctccagctc cccacgattg atctcgttga tcacccacat cagcggcacg
    23041 taggcgatcc cggcacccgc cgttagccag cgcaccagcg tcatcggatc attagtcaca
    23101 aatctccctt gcgggatcag gcgagtcgag atcccttccg gtgcaatcag ttcaaattca
    23161 ttgtcgggcc gcacgctgta ttcaagccat gaatggctac ttaaatcggc gggtttttcc
    23221 ggtatgccgt attgtgtgag atagcttttc gcggcgcaca ccaccattgg catcgcgcca
    23281 agacggcggg aaaacaggct ggaatcctgc aacgcgccga cgcggatcac cacatccaga
    23341 ccgtcggcaa tcaggtcggg ggctggaatt ccggtaacca gattgacgct caaacctggg
    23401 tattctttca gcattttggc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa
    23461 cagccaatgc gtagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca
    23521 tcctgcactt catgaagcat acgacggcag ccctggtagt aaattctacc ggcttcggtc
    23581 aggccaatgc tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt
    23641 gacaccgtct gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa
    23701 gagccaaatt caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac
    23761 tctgacttaa aagtgattta gatcacataa tatagataac agcataacag ttacgctaat
    23821 atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc
    23881 ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag
    23941 tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct
    24001 tttatcactg gcgattttct ggctggtgtg ccgggtactt gtgccaacag gtatctacga
    24061 ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat
    24121 atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg
    24181 ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt
    24241 acaccgaatc cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc
    24301 cggacgtttc tggactcatt acccaggtga atgttcataa ccagctggtg aaaaaaggac
    24361 aggttctgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    24421 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    24481 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    24541 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    24601 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    24661 gtgagtttat tactcgtgga tcaacggcgg ttgcgctggt gaaacagaac tctttctatg
    24721 tactggccta tatggaagaa actaagctgg aaggggtgcg ccctgggtat cgtgcagaga
    24781 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgctgggg
    24841 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctaaccttg
    24901 aatgggtgcg tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    24961 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    25021 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    25081 gatggatatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    25141 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    25201 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    25261 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    25321 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    25381 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtatgc
    25441 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    25501 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    25561 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtgg atcgagagct
    25621 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    25681 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcgc tacaaggcat
    25741 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    25801 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    25861 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    25921 cgcgcaggac gtccacaagc agctcaaacg cctgcgaaga gttatcgcct ggaccgggga
    25981 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    26041 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    26101 cgaaccggaa gtcaaagtag agtcagccga acgtcatcat gcgatggtta acttctggcg
    26161 aaccacactt tcctgcattc tgggaacgct tttctggctg tggacgggct ggacttccgg
    26221 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    26281 acgcatggtg gcgatcgact ttatctacgg gacgctggcc gcgctgccgt tagggctgct
    26341 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    26401 ggcagtgctg ggattcttcc tcggaataga agtacagaaa cggcgactgg gctcgatggg
    26461 ggcgctggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    26521 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    26581 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    26641 ttctgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    26701 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    26761 tcgcctggcg ctgacgatga ttatcgccca ccagcgcctg cgtgatgcgc cgatcccggt
    26821 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    26881 tgcccgtagc gatgataaac gtcgtcggta ctttggtcag ttgctggaag aactggaaat
    26941 ctaccaggaa aagctatgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    27001 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    27061 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    27121 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    27181 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggcgaaagat aaggtacgcg
    27241 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    27301 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    27361 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    27421 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    27481 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    27541 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    27601 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
    27661 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag caacagtggt
    27721 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc
    27781 catatcacaa aatcaagtat acactaatta ttgagctaat taa
//
LOCUS       DAZGXS010000055        27543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGXS010000055 DAZGXS010000000
VERSION     DAZGXS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(92..1459)
                     /gene="rhlE"
                     /locus_tag="ACHKG5_002447"
     CDS             complement(92..1459)
                     /gene="rhlE"
                     /locus_tag="ACHKG5_002447"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0868541.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1688..2359
                     /gene="cecR"
                     /locus_tag="ACHKG5_002448"
     CDS             1688..2359
                     /gene="cecR"
                     /locus_tag="ACHKG5_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0868542.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2362..3357
                     /gene="hlyD"
                     /locus_tag="ACHKG5_002449"
     CDS             2362..3357
                     /gene="hlyD"
                     /locus_tag="ACHKG5_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0868543.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3350..5086
                     /gene="ybhF"
                     /locus_tag="ACHKG5_002450"
     CDS             3350..5086
                     /gene="ybhF"
                     /locus_tag="ACHKG5_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0868544.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5079..6212
                     /gene="ybhS"
                     /locus_tag="ACHKG5_002451"
     CDS             5079..6212
                     /gene="ybhS"
                     /locus_tag="ACHKG5_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868545.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6223..7329
                     /gene="ybhR"
                     /locus_tag="ACHKG5_002452"
     CDS             6223..7329
                     /gene="ybhR"
                     /locus_tag="ACHKG5_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868546.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7291..7701)
                     /gene="ybhQ"
                     /locus_tag="ACHKG5_002453"
     CDS             complement(7291..7701)
                     /gene="ybhQ"
                     /locus_tag="ACHKG5_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0868547.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7834..8595
                     /gene="ybhP"
                     /locus_tag="ACHKG5_002454"
     CDS             7834..8595
                     /gene="ybhP"
                     /locus_tag="ACHKG5_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0868548.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8592..9833
                     /gene="clsB"
                     /locus_tag="ACHKG5_002455"
     CDS             8592..9833
                     /gene="clsB"
                     /locus_tag="ACHKG5_002455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0868549.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9833..10789
                     /gene="ybhN"
                     /locus_tag="ACHKG5_002456"
     CDS             9833..10789
                     /gene="ybhN"
                     /locus_tag="ACHKG5_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0868550.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10825..11538)
                     /gene="ybhM"
                     /locus_tag="ACHKG5_002457"
     CDS             complement(10825..11538)
                     /gene="ybhM"
                     /locus_tag="ACHKG5_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0868551.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11746..12450)
                     /gene="ybhL"
                     /locus_tag="ACHKG5_002458"
     CDS             complement(11746..12450)
                     /gene="ybhL"
                     /locus_tag="ACHKG5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0868552.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12586..13038)
                     /gene="moaE"
                     /locus_tag="ACHKG5_002459"
     CDS             complement(12586..13038)
                     /gene="moaE"
                     /locus_tag="ACHKG5_002459"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0868553.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13040..13285)
                     /gene="moaD"
                     /locus_tag="ACHKG5_002460"
     CDS             complement(13040..13285)
                     /gene="moaD"
                     /locus_tag="ACHKG5_002460"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0868554.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13278..13763)
                     /gene="moaC"
                     /locus_tag="ACHKG5_002461"
     CDS             complement(13278..13763)
                     /gene="moaC"
                     /locus_tag="ACHKG5_002461"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0868555.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13766..14278)
                     /gene="moaB"
                     /locus_tag="ACHKG5_002462"
     CDS             complement(13766..14278)
                     /gene="moaB"
                     /locus_tag="ACHKG5_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0868556.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14300..15289)
                     /gene="moaA"
                     /locus_tag="ACHKG5_002463"
     CDS             complement(14300..15289)
                     /gene="moaA"
                     /locus_tag="ACHKG5_002463"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0868557.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15277..15421)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15686..16594
                     /gene="yvcK"
                     /locus_tag="ACHKG5_002464"
     CDS             15686..16594
                     /gene="yvcK"
                     /locus_tag="ACHKG5_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0868558.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16785..18806)
                     /gene="uvrB"
                     /locus_tag="ACHKG5_002465"
     CDS             complement(16785..18806)
                     /gene="uvrB"
                     /locus_tag="ACHKG5_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0868559.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19385..20062)
                     /gene="bioD"
                     /locus_tag="ACHKG5_002466"
     CDS             complement(19385..20062)
                     /gene="bioD"
                     /locus_tag="ACHKG5_002466"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0868560.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20055..20810)
                     /gene="bioC"
                     /locus_tag="ACHKG5_002467"
     CDS             complement(20055..20810)
                     /gene="bioC"
                     /locus_tag="ACHKG5_002467"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0868561.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20797..21951)
                     /gene="bioF"
                     /locus_tag="ACHKG5_002468"
     CDS             complement(20797..21951)
                     /gene="bioF"
                     /locus_tag="ACHKG5_002468"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0868562.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVCGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21948..22988)
                     /gene="bioB"
                     /locus_tag="ACHKG5_002469"
     CDS             complement(21948..22988)
                     /gene="bioB"
                     /locus_tag="ACHKG5_002469"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0868563.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23075..24364
                     /gene="bioA"
                     /locus_tag="ACHKG5_002470"
     CDS             23075..24364
                     /gene="bioA"
                     /locus_tag="ACHKG5_002470"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0868564.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24423..24899
                     /gene="ybhB"
                     /locus_tag="ACHKG5_002471"
     CDS             24423..24899
                     /gene="ybhB"
                     /locus_tag="ACHKG5_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0868565.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25217..25309
                     /locus_tag="ACHKG5_002472"
                     /pseudo
     CDS             25217..25309
                     /locus_tag="ACHKG5_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25406..26104)
                     /gene="nleD"
                     /locus_tag="ACHKG5_002473"
     CDS             complement(25406..26104)
                     /gene="nleD"
                     /locus_tag="ACHKG5_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFP0868566.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26166..26287)
                     /locus_tag="ACHKG5_002474"
                     /pseudo
     CDS             complement(26166..26287)
                     /locus_tag="ACHKG5_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26335..27216)
                     /gene="nleH1-1"
                     /locus_tag="ACHKG5_002475"
     CDS             complement(26335..27216)
                     /gene="nleH1-1"
                     /locus_tag="ACHKG5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFP0868567.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(27385..>27543)
                     /locus_tag="ACHKG5_002476"
     CDS             complement(27385..>27543)
                     /locus_tag="ACHKG5_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0868568.1"
                     /translation="GLVLVISEYIAPLQDAADLDIATDEQTSLLAAWKQYRMPLNHVD
                     TSVSPDIE"
BASE COUNT         6541 a         7331 c         7019 g         6652 t
ORIGIN      
        1 aaacaataaa atcagacatg tcatgcatgt ctgaccgtgt tacctgaata atgggcaaaa
       61 gcctgtgata ctcttttgcc cttacatggt attactgcgc agcggcaggt ttacgcggac
      121 ggcgacggcg ttgttgttcg cctgccggtt tggcatcgcc aagacgacga gatggttttt
      181 ctgcaggctt cgcgctagct gatttcgcac caccttctcc acggcgtggt tgctgttgac
      241 cgcgaccacc accaccttgc ccacgaccgc cgccgccacg ttgctggcga ccgttctgga
      301 tcggttcggc tttgattgac gggtccggct cataacccgg aatcgcaatg cgcgggatct
      361 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
      421 acaatgcttc gccagttgcc gccgcacggc cagtacgccc gatacggtgg acataatctt
      481 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
      541 gcgcagcgat gtcagttgcc accagtacac gaatatcgcc cgatttaaaa tcggccagcg
      601 cacgggtacg cgcaccttgc gatttattac cgtggatcgc cgcactacgg atgccatctt
      661 tattaagctg ttcagcgaga tggttagcgc catgtttggt acgggtaaac accagcacct
      721 gctgccagtt ccctttccca atcatgtgcg acagcaattc gcgcttgcgt ttcttatcaa
      781 caaagtgaac atgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttctattt
      841 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcgg
      901 agaacaacag gttctggcgc ttcgcaggta gttttgttaa cacgcgacgg atatcgtgga
      961 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
     1021 gcttcactgc attctgatgt tccaggtcca gcaaacgtcc cggcgttgcc accagcacat
     1081 caacgccgcc gcgcaacttc atcatttgtg ggttaatgct gacaccgcca aacaccacca
     1141 gcgaacgaat gttcaggtat ttgctgtaat cacggacgtt ttcgccaatc tgcgccgcca
     1201 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
     1261 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgttttg ccggtgccgg
     1321 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
     1381 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
     1441 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
     1501 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
     1561 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
     1621 catactggac aactcccata tggagtcata atcttaatca atcatttgat taagaatgga
     1681 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
     1741 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
     1801 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
     1861 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcatgc
     1921 cgaggaagcc gaacgcttgt tcgcccaacc acagcctgat cgggctgcca tccgtgaact
     1981 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
     2041 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctaccacc tggtgcacga
     2101 acaggtgatt agtccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
     2161 cgacgccaat gacacccgca tgatccttca tacccatgcg ttgattggtg agattctggc
     2221 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
     2281 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
     2341 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta
     2401 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
     2461 acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt tggggggcgc
     2521 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
     2581 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggcgt ctcggtggca
     2641 caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
     2701 gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta taaccgccag
     2761 caaggactgt ggaaaagccg caccatttcg gcaaatgacc tggaaaatgc ccgctcctcg
     2821 cgcgaccagg cacaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
     2881 ggtaaccgtg aacaggacat cgctcaggcg aaagccagcc tcgaacaggc gcaggcgcaa
     2941 ctggcgcagg cggagctgaa tttacaggac tcaacgttga tagcaccgtc tgatggcacg
     3001 ctgttaacgc gcgcggtgga gccaggcacg gttctcaatg aaggtggcac ggtgtttacc
     3061 gtttcactaa cgcgtccggt gtgggtgcgt gcttatgttg atgaacggaa tcttgaccag
     3121 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccctatcac
     3181 gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
     3241 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
     3301 ttacgccagg gaatgccagt gacggtacaa ttcggtgacg aggcaggaca tgaatgatgc
     3361 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
     3421 gccgctcgat tgcaccattc atgccggtta tgtgacgggg ctggtagggc cagacggtgc
     3481 gggtaaaacc acgctgatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
     3541 cacggtgatt ggctttgatc cgatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
     3601 tatgccgcag aaattcggcc tgtatgaaga tctgacggtg atggagaacc tcaatctgta
     3661 cgcggatttg cgcagcgtca ccggcgaggc acggaagcag acttttgctc gcctgctgga
     3721 gtttacgtct cttggcccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
     3781 acaaaaactc ggcctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
     3841 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
     3901 ggcgggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
     3961 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggagaac caaaagccct
     4021 gacacaaacc atggccggac gcagctttct gatgaccagt ccacacgagg gcaaccgcaa
     4081 actgttgcaa cgcgccttga aactgccgca ggtcagcgac ggcatgattc aggggaaatc
     4141 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgacgggat
     4201 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcgttta ttgatttgct
     4261 gggcggtgcc ggaacctcag aatcgccgct gggtgcaata ttgcatacgg tagaaggcac
     4321 acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg attttgccgc
     4381 caccgatcac gtcaactttg ccgttaaacg cggggagatt tttggtttgc tggggccaaa
     4441 cggcgcgggc aaatcgacca cctttaagat gatgtgcggt ttgctggtgc cgacttccgg
     4501 ccaagcgctg gtgctgggga tggatctgaa agagagttcc ggtaaagcgc gccagcatct
     4561 cggctatatg gcgcaaaaat tttcgctcta cggcaacctg acggtcgaac agaatttacg
     4621 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
     4681 gagcgaggca tttggcctga aaagtattgc ctcacacgcc accgatgaac tgccattagg
     4741 ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggata ttctgtttct
     4801 cgacgaaccg acttccggcg ttgaccccct cacccgccgt gaattctggc tgcacatcaa
     4861 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcgga
     4921 atattgcgac cgcatcggcc tggtgtaccg cgggaaatta atcgccagcg gcacgccgga
     4981 cgatttgaaa gcacagtcgg ctaacgatga gcaacccgat cccaccatgg agcaagcctt
     5041 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctttcct
     5101 ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca gatcgttcgc gatccgagta
     5161 gctggctgat tgcggtagtg atcccgctgt tgctgctgtt tatttttggt tatggcatta
     5221 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcagcgc
     5281 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
     5341 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattcccg
     5401 tggatttcgc cgaacagatg gagcgcgcca acgctaccgc accgattcag gtgatcaccg
     5461 acggcagtga gccgaatacc gctaactttg tgcagggata tgtcgaaggg atctggcaga
     5521 tctggcaaat gcagcgggcg gaggataacg ggcagacctt tgagccgctt attgatgtac
     5581 aaacccgcta ctggtttaac ccggcggcga ttagccagca ctttattatc cccggtgcgg
     5641 tgaccatcat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
     5701 gggaacgcgg cactatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
     5761 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
     5821 tgtcagtgtt tattctcggc gtgccgtatc gcggttcgct gctgattctg ttttttatct
     5881 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
     5941 accagttcaa tgccgctcag gtcgccctga acgccgcgtt cctgccgtcg attatgcttt
     6001 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
     6061 ttcccgcacg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
     6121 tggtgctggt ggtgaacgta ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
     6181 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
     6241 acgttaatcc gcaaagagtt gcagtcgttg ctgcgcgaac cacaaacccg cgcgattctg
     6301 attttaccgg tgctgattca ggtgattctg ttcccgttcg ccgccacgct ggaagtgacc
     6361 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
     6421 cgttttgccc gcgccagcgc ctttacacat gtgctgctgc tgaaaagccc acaggagatc
     6481 cgcccgacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
     6541 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
     6601 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
     6661 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
     6721 ccgaatctcg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatt
     6781 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
     6841 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
     6901 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
     6961 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta tggtttatcg
     7021 ctggtgggat tcggtctgtt gatttcatca ctctgttcaa cacaacagca ggcgtttatc
     7081 ggcgtgtttg tctttatgat gcccgccatt ctcctttccg gttacgtttc gccggtggaa
     7141 aacatgccag tatggctgca aaacctgacg tggattaatc ctattcgcca ctttacggac
     7201 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
     7261 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
     7321 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
     7381 cagccctgca attgccagca aaaagttgtt ttccagtacg cgggacaaca gccacagcac
     7441 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
     7501 acgccagcgt tgctcggggt ggcgctgaaa caccagcatc atcactaccg tgacacaata
     7561 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
     7621 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagacccg ttgcgacacg
     7681 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
     7741 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
     7801 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
     7861 ttcaaggtgc tcaccatcaa tattcacaaa ggctttaccg cgtttaaccg acgcttcatt
     7921 ttgccggaac ttcgcgatgc cgtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
     7981 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
     8041 tacgagtttc tcgccgacac tatgtggagc gattttgcct acggtcgcaa tgccgtatac
     8101 ccggaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
     8161 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgccg
     8221 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
     8281 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
     8341 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
     8401 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
     8461 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
     8521 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
     8581 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
     8641 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
     8701 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca actgcatgcg gcactactgg
     8761 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
     8821 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt agtgttccgc tactacgatc
     8881 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
     8941 tggtaatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
     9001 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
     9061 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
     9121 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
     9181 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
     9241 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
     9301 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
     9361 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
     9421 gcggcgttca ggtgtttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
     9481 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
     9541 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
     9601 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
     9661 ggtggaacct aaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
     9721 ttggctggct tccggcacac acgccacgtc tggcgcaggt tgatcctccc gcacaaccga
     9781 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
     9841 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
     9901 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
     9961 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    10021 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    10081 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    10141 cggcggcatt ggtatgcgct atcgtttgta ctcccgtctg ggattgccgg gcagcactat
    10201 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    10261 gattatcttt accgcaggcg tggtggagtt gccggaccac tggtatgtcg atcaaactac
    10321 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    10381 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcgtggaa
    10441 attcgccctc gcccagatgc tgatttccag cgttaactgg atggtcatgg gggcgattat
    10501 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tgctggttag
    10561 cagtattgcc ggcgtcatcg tgcatgttcc ggcggggatt ggtgtgctgg aagcggtgtt
    10621 tatcgcgcta ctggctgggg agcatacatc caaaggcaca attatcgccg ccctactcgc
    10681 ttaccgtgtg ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgttgct
    10741 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    10801 gttctttagt atgtgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    10861 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    10921 tgcacctgta aaagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    10981 caattcaagg agcttatttc gtccatgcaa gataatgcct gaccataaca caatcattaa
    11041 gcaacttatt acccaaatgg gccgttcact cattaatgcg gcattcacga tgattaccag
    11101 ggccaaccct gtcaacgtca tcataatcat aaaacggtga gaaccaggat caatattaaa
    11161 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    11221 aatggcatat acaggaacta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    11281 atatattacg cctgcgatgg cggcatttat cttgttgata tccttaatta agccatacat
    11341 acaaagtaat aataacccta tggcgacata cattggtggt gaatcatatt tggttgccgt
    11401 aatttcgaga tacttagccc cccaggccag ccctgcggtt gccaccaaac ctaaagcggt
    11461 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    11521 ttgcaagtat gattccattc ttttagttgt ccttatagaa aaatattaat attcatcatg
    11581 aataaattta aacccacgca tacaaaatac ttttgtattt atggatacgg aaaactcttc
    11641 cgctcggcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt ttagtaagaa
    11701 aaaaaatccc gacacatggc cgggatattg atggtgaaaa aagaattaac gacgattgcc
    11761 aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg ttaatgcgca
    11821 aagaatggaa tatttgcgca ggttcgacgc gtcgcgggta tctatctgct cacccatatt
    11881 tttcagtttc tgggtgtcat acgccgtcaa tccgacaaag acaatcacgc cgatgtaggt
    11941 gactgcccac atcaacgctt cgcttttcag ccagaagttg accagcgatg ccaacacaat
    12001 gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct tcgtggtgta
    12061 accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac tggcgataga
    12121 agcagcggta taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag
    12181 cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat
    12241 aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag tgaacaacag
    12301 ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaataagcc
    12361 aacggtcatc cagccataga cttgcgccat ataagtctgc aagccagccc ggggttgtac
    12421 aattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct
    12481 taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc
    12541 ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaactacc agcgttttgc
    12601 cgcctgctga tcgctctccc gagcttcgac ccagcggtcg ccctccggcg tggcttcgcg
    12661 cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg cttcaaacgc
    12721 actgctgcga tgcgcactgg tgacaccgac aaaaacgatt tcatcgcccg gccataattc
    12781 cccgatgcgg tgaatcaccg tgacgcgccc cagcggccag cggttacgcg cttcatcgac
    12841 aatttctgcc agtgcttttt cagtcatccc cggatagtgt tcgagggtta atgctttgac
    12901 gctgtcgccc aggttatggt tgcgcacctt accggtaaag gtgactaccg caccgtcttc
    12961 gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg gaccaacaac
    13021 aattttggtt tctgccatct taacctccgg ttaccggcgg gaagaaagct acttcgtcgc
    13081 cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc agtaatttgc
    13141 catcttccag cgccagcgcc cagcgatcgc tctgcgcagc caggtgctgg cgtaacgctt
    13201 caacggttgg gaaatccgca gccacttcgg ttgcatctgt tcctaccaac tcgcgcacct
    13261 gggcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga cttgccgccg
    13321 ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc tttgcacatg
    13381 tcataaatgg tcagcgccgc cacggaggcc gcggttaacg cttccatttc gacaccggtt
    13441 ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg ctcggcctgt
    13501 aaattgactt caactttgct gagcatcagc ggatgacaca gcgggatcag atcccaggtg
    13561 cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg
    13621 cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc
    13681 gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc gccagcggcg
    13741 ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa ttacacggac
    13801 gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg cacgctttgg
    13861 tcgaacccgg catggcgaaa atcagcgttt tgttagcgac gcccgctacc gcacgagatt
    13921 gcaacgtgga agtgccaatc tcttcaaacg acaacatacg gaacacttca ccaaaacctt
    13981 caacttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc
    14041 cagtcccccc cgtaatcaac accacttgta catcgtcgct ggcgatccac gcagatacct
    14101 gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg tgatggcccg
    14161 cttcttgcgc cgaatcgcgc aaatagtgac cggaggtatc gtcttcttca ccgcgacgat
    14221 tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc tgactcatct
    14281 gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta ataccggtgt
    14341 tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa atacgcgctt
    14401 ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg ccgccttcac
    14461 caaacaggca gagatggagt ttaccaatgg aggaaacgcg cagacggttg caagtggcgc
    14521 agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatcc ggatgacaaa
    14581 agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg cgacgcagta
    14641 gctcgtcacg cagaacctga ccagagatgt gatgcttacg gaaaagctcg ctgccctcgc
    14701 ccgtttccat cagttcgatg aaacgcagct ggatagggcg atgctggatc cagttcagaa
    14761 aggtgtcgag ctggtgatga ttcacatcac gcatcagcac ggtattgact ttgaccttct
    14821 caaaaccggc ctcaaatgca gcatcgatcc ctgccatcac ctgattgaat ttatcctggc
    14881 cggtaatggc atgaaactgg cgggcgtcca gactgtcgac gctgacgtta atgccagtaa
    14941 gtcccgcatc gcgccagttc gccacatcgc gttccagacg gtaaccattg gtggtgaccg
    15001 ctatctggcg aatagcgtcg ttttctcgca cagcggcgat gatatcggta aagtcgcggc
    15061 gtaaagacgg ctcaccgcct gtcaggcgca ctttttcggt gcccagactg gcgaaggcgc
    15121 gcgtaacccg gcgaatttca tcgacggtca gaaagccttt attggtgacg ccgctcggtt
    15181 tgtagccatc cggcaggcag taggtgcaac gaaagttaca cacatcggta atcgacaggc
    15241 gcaagtagta aaacttacgc gcaaatgcat cagtcagttg tgaagccatg tacacctttc
    15301 cagatacggg aggcgaagtc atttcttcct tcgccctggt ggcaatcttt acgagcattg
    15361 tcacggccaa agcaccatat cagttgaccc aggtgcagag gctagagtgt ttagtggtta
    15421 agccgatact agcgtgtaaa tgagattttt accatccaca ctttcgctat gtaatcatgt
    15481 atatagcgtc atgatcgtgc tatttcgcta cagaataagg gaaaatggcg gttttacttt
    15541 gatatacatc atcagggagg catagcgtgc atcgtccttt agaggtagaa gaaaatagtt
    15601 tgtatcctca atattggcag gttaattgct gtttccccgc aatttgcgct actgtagcgc
    15661 ggataatctg actccaggaa actatatgcg caatcgtacg ctggctgatc ttgatcgtgt
    15721 cgttgctctc ggcggagggc atggactggg acgcgttctc tcatcacttt cgtctttggg
    15781 ttctcgttta acgggtatcg tcaccaccac ggataatggt ggctcgacgg ggcgtattcg
    15841 ccgttcagaa ggcggcattg cctggggcga tatgcgcaac tgccttaacc agctgataac
    15901 tgaacccagc gtcgcttcag caatgtttga atatcgtttc ggcggtaatg gcgaactttc
    15961 cgggcacaac cttggaaacc tgatgttgaa ggcactggat caccttagcg tgcgacctct
    16021 tgaagcgatc aatctgattc gaaatctgct gaaagtggac gcacatttga ttccgatgtc
    16081 ggagcatcct gttgatctga tggcgattga cgatcagggg catgaagttt acggcgaggt
    16141 caatatcgac cagttaacta cgccgattca agagttattg ttaacgccta atgtacccgc
    16201 aacgcgtgag gcggttcacg ctatcaatga agcggatctc atcattattg ggcctggcag
    16261 tttttacacc agcctgatgc caattctgct gctgaaggaa atcgcgcagg cattacgccg
    16321 cacgccagcg ccgatggtct atatcggcaa tctggggcgt gagttgagtt tacctgcggc
    16381 caatttgaag ctggaaagca agctggcaat tatggagcag tatgttggta aaaaggtcat
    16441 tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc ggattgtgat
    16501 tcaggaggtg ctggaggcca gcgatattcc gtatcgtcat gaccgccagt tgttacatag
    16561 cgcactggaa aaggcgttac aggctttagg ttaacggaaa tccgaaggaa aattccggct
    16621 tcctaatgaa gacaaagtgc gcgttgttta tgacggatgc ggcgtgaacg ccttatccgg
    16681 cctacaaacc gcgctaattc aatgtattgc agaatcatgt aggcctgata agcgtagcac
    16741 atcaggcaat tttgcttttg tcatctgtct tcttcgttat cctgtcacga agccgcgata
    16801 aacaattcac gcagctgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga
    16861 ttttgcgcgt gttgcatcat taacccttcc agctcatgga ttttctgttg caacgcttta
    16921 ggcgacatat ccatcggcac attatccggc tcaacaatcg gtcgcgattt tcctctgccc
    16981 ttcgctttgg ttttagcaat gttctgcccc aacgccagga tatcgaccac tttcttgttc
    17041 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt
    17101 tcggtttcgc caatcgcttt cgccattgac ggggtgatct tatcgccgta gagaatcgct
    17161 ttaccgttaa cgttacgcgc cgcacgacca atagtctgga tcaacgaacg ttcagaacgc
    17221 aggaagcctt ctttgtcggc gtcgaggatc gccaccagcg aaacctccgg catatccaga
    17281 ccttcgcgca gtaagttgat ccccaccagc acgtcgaact cgcccagacg caagtcgcgg
    17341 ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcaccg
    17401 tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt ggtgaccagt
    17461 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
    17521 gccactggcc gcacttcgat aatcggatca agcaatccgg ttggacgcac cacctgatcc
    17581 accacatcgc cgccggattt ttccagctca taattacctg gcgtcgccga aacatagatg
    17641 gtttgcggcg ctaatgcttc gaactcttca aatttcagcg gacggttatc cagcgctgat
    17701 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
    17761 ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcggcaggc
    17821 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
    17881 tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta
    17941 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttgcgtctg
    18001 gcggccagct cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg
    18061 tagtgcgttt tcgggtagat ggtaaaacgt ggaatggtgg aaacaatctg cccggttagc
    18121 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg
    18181 tcatccgatt ctgccgggaa gatatcaatc acctcgccac gaacacggaa agtaccacgc
    18241 tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc
    18301 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
    18361 cccagaccat aaatcgcgga aacagacgcg accacaacaa catcacgccg ctccagcatc
    18421 gctttggtgg cggacaaacg catctgttcg atatgttcgt ttaccgaggc atctttctca
    18481 atgaaggtgt cggaactcgg tacataggct tctggctgat agtagtcgta gtaggagacg
    18541 aaatattcca ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc
    18601 agcgttttgt tgggcgcaag taccatggta gggcgctgaa ggtcagcaat gacattggca
    18661 atggtgaagg ttttcccgga accagtcaca ccaagtaacg tctggtgcgc caggccatct
    18721 tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg
    18781 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
    18841 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
    18901 catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgag taaaatttac
    18961 tctgtggcga gataaaactc ggaccttacc gggttatccc caaagcaacg gcttttttaa
    19021 catttgtcaa gatgagtgat gtcagtttta tggcaagaga tgcctgttca gtgactgcca
    19081 ttgcaattat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
    19141 attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
    19201 ggttatccac aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa
    19261 ttcccacatt cgccgcatga tgcggaagta aaattttttt cgtgattatt ttagaattta
    19321 attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacagg
    19381 atggctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg
    19441 caagccaggg gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
    19501 attcagcgtg acgttttccc ggaggcgtga catcgttcgc cacccaaccc gccagagtca
    19561 gcccggcgtg ttgtatcgcc tgtgccgtca acatcgcgtg attaatacag ccaagtttca
    19621 cgccaaccac cagtattacc ggcagttgtt cctgtgttac ccaatccgca aaagtgaaag
    19681 tgtcagaaag cggcgtaaac cagccgccag cgccttccac taacacccag tcagcctgtt
    19741 gttcaagcgc gcgtaatccg gagctcatta ccgatgattc tatcgacctg ccctcttgcg
    19801 cgctgatgat gtgcggtgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat
    19861 ccagctgcag gctgctgttg cgctgtaacg ccagcgcgtc actattacgt aaaccctccg
    19921 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgatag cctgctgcct
    19981 ttgcggcttg taaaagtgca caactggcga cagttttccc cacttcggta tccgttccgg
    20041 tgacaaaata acgtttactc acgaacaatc actcccaaaa aaagatgata cgtcagagga
    20101 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaactgtga acgcgttaat
    20161 attcgctggt cgcgcccttc atgaagatgc gtggcaccga tgcctttcag cgaacgcatg
    20221 gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tacgatgttg ataatgcacg
    20281 ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg cgggcgctcg
    20341 tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
    20401 gcgaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt tgataaatta
    20461 ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
    20521 agggattcga tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcgca
    20581 agcattggcg gcgagagatc taacgccgtc acctgcgcgt gacgctctcg ccagtggcgg
    20641 ctcatccagc caggcccaca ccccgcgtcc agtacgtggg ggtatttacg ctgtgggagc
    20701 attgccagta agacatcagc actctggcgc tgtaactctg cgtgttgctc atagtgcgcg
    20761 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
    20821 cagcagacgg tcgatatcct gcatttcatg cgcggcggtt agcgttaagc gcagtcgcgc
    20881 agtaccagag ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
    20941 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
    21001 gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgtaatgag
    21061 tgccgccagt ttttcgcgcc gtgcatcacc ctcatcactg cgaatgaccg ccagtgacgc
    21121 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataagat ggcgggcgaa
    21181 ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccgc agacgccaaa
    21241 tcctttgcca aaagtcacta ccagcaattc tggttttacc ttttgcaacc agcaggtgcc
    21301 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
    21361 gtgctgttgc gttacctgct ggatttccgc cagtggcgca ctatcgccgt ccatgctgaa
    21421 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
    21481 atgagtgaca tcgttatgaa caaaacggcg aagctgcgac gggcttaaac tggcagcctc
    21541 cagcaatgag gcatggctaa gccggtcagc gacaatgcgg tcctcttttg ccatcatcgc
    21601 ggcaatgacc gcctgattag cggcaaaacc agagataaat agcagtgccc gcgaatagcc
    21661 cagccactcg gccagttctt cttccagcgc ctgatgcgca acgctataac cgctgacgtg
    21721 accggaaccg ccgctaccga cgccaaattg ctccgcaccc tgcttccagg cacggataat
    21781 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    21841 gtccgccacc agccagcgtc cggctccttg cgccactgga taacggcgac gcagggcatc
    21901 ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc
    21961 gcgttgtaat attcgtcagt gtccggggtc atcagtgcct gctccagacg ctgctgttgt
    22021 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcgaaacagt
    22081 tgcaggtctt tatcttcttc cggattgggc gtggttagca gtttgcaacc gtagaaaatc
    22141 gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg
    22201 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat ggtgcgaata
    22261 aaatcaaagg catcgacatc atcgttatct gccagtggag tacctttcac cttcaccagc
    22321 atgttgattg gcacgctttc cggcggcgtc ggcaggtttg ccagttgcag taataatccg
    22381 gcgcgatctt ttaccgtttc gcctaaaccc acaatgccac ctgagcagac tttgatcccg
    22441 gcatcgcgca ctttttccag cgtatcaagg cgttcctgat aagtacgtgt ggtgatgata
    22501 ttgccgtaaa actccggcga ggtgtcgagg ttatggttgt agtaatccag cccggcattc
    22561 gcaaggcgct gcgcctgaga ttcactcaac gtacccagcg tcatacacgc ctccagcccc
    22621 atcgctttta ctccctgcac catttgttcc aggtatgaca tatcgcgttc gtggggattc
    22681 ttccacgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac
    22741 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag
    22801 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaaggtg
    22861 ctgacctgca cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc ttcaaacagc
    22921 agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg
    22981 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa
    23041 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    23101 ccaacgccat atctggcacc catacacatc catgacctcc ccgctaccgg tttatccggt
    23161 ggcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat
    23221 gtcgtcctgg tgggcggcga tccacggtta caatcacccg cagcttaatg cggcgatgaa
    23281 gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgccagccat
    23341 tgagctgtgc cgcaaactgg tggcaatgac gccgcaaccg ctggaatgcg tttttctcgc
    23401 ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaagc
    23461 caaaggcgaa gcgcgccagc attttctgac cttccgcaat ggttatcatg gcgatacctt
    23521 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    23581 gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgagcg
    23641 cgatatggtg ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cagcggtgat
    23701 cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    23761 acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    23821 cggatttggt cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggacat
    23881 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac
    23941 gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc tgctttatgc acgggccaac
    24001 ttttatgggc aatccgctgg cctgtgcggc tgcaaacgcc agcctggcga ttctcgaatc
    24061 tggcgactgg cagcaacagg tggcggctat tgaagtacag ctgcgcgagc aacttgcccc
    24121 cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg ggggccattg gcgtagtcga
    24181 aacaactcgt ccggtgaata tggcggcatt gcaaaaattc tttgtcgaac agggtgtctg
    24241 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    24301 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    24361 ataacgggaa gtcagcgtga gagtttctgg ctacactttc tgcaaactag aaaggagggt
    24421 ttatgaaact catcagtaac gatctgcgcg atggcgataa gttgccgcat cgtcatgtct
    24481 ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc
    24541 ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg
    24601 gctggtggca ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaagggt
    24661 ttggctctgg tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta
    24721 aaaccggata cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg
    24781 ttcacgcgct ggatgtagag cgtattgatg tcgatgaagg agccagcggc gcgatggtcg
    24841 ggtttaacgt tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat
    24901 cactctgcca gatggcgcaa tgctatctgg tatcacttaa aggtattaaa aacaactttt
    24961 tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg cgaaacagca
    25021 aaacccaata atatcaatgt attacaatgc atttagtcta aaaaatagac tgcataatgc
    25081 tacaaaacac aacatatcca gtcactatga atcaactact tagatggtat tagtgacctg
    25141 taacagagca ttagcgcaag gtgatttttg tcttcttgcg ctaatttttt gtcaaacaca
    25201 ttacgtcgtt tgctgaattt acaaccatgc tcagtatctc tataagcgca gagaaatgat
    25261 gcagtggtgg gtgggctggc ttgatgaaaa ggtggggtac tccacctgaa ccactatcgg
    25321 aatagcacaa agccttgcaa tccagtgcaa agttttgtgt gcctccgttt gccccaccaa
    25381 tatatattca gcacaagaaa cacagctaaa gcaatggatg cagtcttacc tgttggaatc
    25441 ccagactcac tgtattgtca tcatgaataa gagctgagtc gtgcgggtag gaggttctac
    25501 ggggcatccc aatctcttcg tggaatttat tttctgaaag cacctcctct gaaaaagccc
    25561 ccaacccaac agtcctggct tcttcgagta aaagtggaga gtatttttgt gattctgctc
    25621 gggaactctc aactttcaaa cgctccccat ttaaattgtg gaaaacatgg agcaactcat
    25681 gaaaaacaat agtcgcatga aagtccacca cgctaatccc ctccccacag ggatattcaa
    25741 ctgcattcag attacagtga aaatcggaac cagtcccccg atggttctca gcatctatat
    25801 ctctatgtgc cataactcct agtctggaag agttgaggtg tattaccact gtttctgatt
    25861 taagtcggga tattgattca atagcattca ggagtacacg ccctgtctct gtagagccaa
    25921 tcttatcgag tgctgcttca atctgctgat atatattatg atcagtcccc tggaaacgaa
    25981 gttgcggatt attttttatc cattgaacgc tcgatgcttt tacaagagac tcgatatctg
    26041 tatctgacat tgagctcgac cttgatgact gatgtaatac caagttgagg gacgtagggc
    26101 gcatcaggac tcctgagaat gctacttatt agcagcctgt ttaccactct gtgaaaatga
    26161 tttttttaaa agtgaccgtc aaggcggtca ccaaacccga taataaagcg gatcatagcc
    26221 atttgccagt cctcagcgac attgcccatt tccgtgatcc gccaagaatt gccaaccaca
    26281 caatttgtta ccaactccat ccatgcaaca aatccactac actggataaa attactaaat
    26341 tttacttaat accacactaa taagatcttg ctttccgcca tgataagatt gcattatttg
    26401 actttcactc catgaacggt cggaaacatt ataagatgat atatctattg gattaaactc
    26461 attcttcgcg cggtcatata agacatttgt ctctgttgta tcgacaaaaa gaattccttt
    26521 ttgctccagt ctatcaaaca tatcgtaaat agcatgctca gcctgtgctg gcaaggacga
    26581 aatatttaaa agcgattctc catttatttt atccattcta ataccaataa tatcaccatt
    26641 attgccatat attttttctg cactcccggc accataatat tggttgaagc aacgaacttc
    26701 gcttgtcacc tcttcattgc tttgagatgt agtaaacatc ttcaggactt ttgttgcatc
    26761 ctcagcatct tcatatacta cagcgttccc ccctttgcca ataacattac caggtactga
    26821 caacctgtta tagtccacac tcggtaactc tggcggtgca taatcaacag gaggtaaatc
    26881 aggtctgttc gaatgaacaa tgcctctttc catagcagcg ctcactggtg atgcattcag
    26941 catgacctca attttcctgc taatctcccc cttaggccat cccagcctgt gcaacagatt
    27001 agtaaaacaa ccactatgac tttctcttgt aacgcaaaac ttattatcgg tggcaacaac
    27061 acgatatgtt ctgttgccaa cctttacttg cgccccatta tcagaatgaa cggcagcatc
    27121 ccttacagag gataaaacac gattatcagg cgaagtcagg tttctggtta aagaattcca
    27181 tgaacatccc aaatttacag aatatggcga taacatacat ttcaaccttc aaaataaacc
    27241 catctaaatt tccttaacaa acatccccct aacatggcaa caaaaattag aaatgaactc
    27301 ttgctttttg tatctggcaa ctaattcatc cttccagcat cccatcaaac gaacgtttac
    27361 gaactataac gcaggtatta ccggtcactc gatatctgga gatacagacg tatcaacatg
    27421 attgagcggc atacgatact gcttccatgc cgccagtaac gatgtctgct catccgtcgc
    27481 aatatctaaa tctgcagcat cctgaagtgg tgcgatatac tcactgatta caagtaccag
    27541 acc
//
LOCUS       DAZGXS010000056        27130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGXS010000056 DAZGXS010000000
VERSION     DAZGXS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1273
                     /locus_tag="ACHKG5_002477"
     CDS             <1..1273
                     /locus_tag="ACHKG5_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFP0868569.1"
                     /translation="AAPLPQWANAPAVTPVAQLSLQESILRAFARNPGVTQQAAQIGI
                     GEAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYDFGKTNNDI
                     NLQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLENVYNMAAL
                     RANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMASAKAQLAVLTGVQPEAIAAPPAEL
                     AEQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKTRYQTSDRS
                     YWDDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRASVAYANWTG
                     ARGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAEINANYDGW
                     VAAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
     gene            1376..5761
                     /locus_tag="ACHKG5_002478"
     CDS             1376..5761
                     /locus_tag="ACHKG5_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0868570.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDFTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            5813..21535
                     /locus_tag="ACHKG5_002479"
     CDS             5813..21535
                     /locus_tag="ACHKG5_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0868571.1"
                     /translation="MATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNYTATTD
                     AAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTINPVAT
                     DDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPAADIQA
                     IGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQALVIT
                     GSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVSGTNTA
                     GTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQTVTVTF
                     GGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYSVDATA
                     PTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQADGSWSV
                     SLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDDIINAA
                     EHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALGSGPQT
                     ITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGTSNQPA
                     GTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNSNSASH
                     NVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGNTYTAT
                     VQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTV
                     AGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGVPAVDV
                     SNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEKGETLV
                     VSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQASVSN
                     INGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQTLTVT
                     LNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHALVVDIT
                     APDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLDASGNW
                     SVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSGDDIIN
                     AAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQALGNG
                     ELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVITGSSS
                     GLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNTAGTTT
                     SISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVIFGGKS
                     YTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDATAPSVT
                     INTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWSVSVPT
                     GDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINATEHGQ
                     AQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDVTITAT
                     VTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQPAGTQI
                     TVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSASHNVQV
                     NTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTATVQAN
                     LSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDTVAGDD
                     VVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVDVSAWP
                     AGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAALTLSGS
                     TSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVSNVNGN
                     SATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTVTLNGT
                     NYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDTSVLVV
                     TINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGNWSVGV
                     PASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNILNADEK
                     GQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQANYTVS
                     ASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVTRAAAG
                     DTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTGSGTRD
                     ITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVAYSATV
                     QADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAISINTVA
                     CDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVPAADLA
                     TLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAGSPLTI
                     SGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSATVNDKA
                     GNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDVVSVVL
                     NGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVTVSLSA
                     PVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDASGNWSV
                     TVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDDIINAS
                     EAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALGNGELT
                     VNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGSSSGFA
                     AGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGTQTSIT
                     HPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGGKTYTT
                     TVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPTVTINT
                     IASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTLPASDL
                     TALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEHIQAQI
                     ISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTISATITD
                     SAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATGTVVTV
                     LLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASRSVAVD
                     LTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATVAANGT
                     WALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVAGDDII
                     NNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAGLSDGS
                     YTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVISGTTTA
                     PVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAIGNTGS
                     SNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNETAQIS
                     IDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVIDTTAP
                     DPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGGVTWTT
                     LTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSITIT
                     GISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAADSLNW
                     SYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTDTGSSA
                     TDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRTLTDGS
                     YVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATDDT
                     TPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARVVD
                     LAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVING
                     KTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANISNG
                     TVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTALT
                     LYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVGTL
                     TWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVGGA
                     VTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNTFL
                     SGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAVGAT
                     ATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQSGTTSG
                     VAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYGNTVKL
                     YNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQTV
                     NTSWGGLQATDATPAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWVYS
                     SGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFNDIL
                     TGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWEGT
                     ADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQIDR
                     DGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            21539..23701
                     /locus_tag="ACHKG5_002480"
     CDS             21539..23701
                     /locus_tag="ACHKG5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP0868572.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            23698..24873
                     /locus_tag="ACHKG5_002481"
     CDS             23698..24873
                     /locus_tag="ACHKG5_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP0868573.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            24870..25277
                     /gene="cueR"
                     /locus_tag="ACHKG5_002482"
     CDS             24870..25277
                     /gene="cueR"
                     /locus_tag="ACHKG5_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0868574.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(25281..25484)
                     /locus_tag="ACHKG5_002483"
     CDS             complement(25281..25484)
                     /locus_tag="ACHKG5_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868575.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(25481..25903)
                     /locus_tag="ACHKG5_002484"
     CDS             complement(25481..25903)
                     /locus_tag="ACHKG5_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0868576.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(25988..27004)
                     /locus_tag="ACHKG5_002485"
     CDS             complement(25988..27004)
                     /locus_tag="ACHKG5_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0868577.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         6203 a         7883 c         8001 g         5043 t
ORIGIN      
        1 tgctgcaccg ctgccacaat gggcgaatgc cccagcggta acgccagttg cccaattatc
       61 tttacaggaa agtatattac gcgcctttgc gcgaaatccc ggtgtcactc aacaggcggc
      121 gcagataggt attggcgaag cgcaaattga tgaagccaaa agtgcctggt atccgcacgt
      181 tggattaacc ggcaacgcgg ggccgtcccg acaaacggac tccagcggca ggcttgataa
      241 caacgtttcg tatggcataa ccctgacaca actggtgtat gactttggta aaactaacaa
      301 cgatatcaat ctgcaaactg ccgcccgtga cagctaccgc tttaaattga tggcaacctt
      361 aaccgatgtt gcagagaaaa cggcgactgc ctacatggaa gtcagtcgtt atcaggcttt
      421 gtgcgatgcg gctcaacgca atattcactc gctggaaaac gtctacaaca tggcggcatt
      481 gcgcgctaac gcaggcctga actcatcgtc ggatgaatta caggcccaga cgcgtattgc
      541 cggaatgcgc tcaacgctgg agcaatatca ggcgcagatg gcgagcgcca aagcgcaact
      601 ggcggtgctc actggcgtac agccggaggc gatagccgcg ccacctgctg aattagccga
      661 gcagccggta tcgctgaaga atattgatta ccagtccatc ccgctggtgc tggcggcaga
      721 aaacttacgc cagtcagcac agtacggcgt ggaaaaaacg aaagcgcaat actggccaac
      781 gctcagtatt caggggggta aaacgcgcta ccagaccagc gaccgctcgt attgggatga
      841 tcagctacaa ctgaacgtta acgcgccgct gtatcagggc ggcgcggttt ctgcccaggt
      901 gcaacaggcc gaagggcaac aaaaaatctc tgcctcgcag gtcgaacagg caaaactgga
      961 tgtgctgcaa cgagcgtctg tggcatatgc gaactggacc ggcgcacggg gtcgtgaaga
     1021 agccggttta gcgcaatccg aaagtgcgca caaaacgcga gatgtgtacc aaaatgaata
     1081 taaattgggt aaacgcagtc tgaatgatct attaaccgtc gaacaagatg tctttcaggc
     1141 gcaatcggct gaaataaatg ccaattatga tggctgggtc gccgccgtaa attatgccgc
     1201 tgcggtgaat aaccttattc cattagcggg aattaaacag ggcttataca acgacttacc
     1261 cgacttgaaa taatcctgcc attattaata gtgggccgtt gttaagtgat tacctttgtc
     1321 ttatttagat aaatggactc gctaaggatg tgcgccattt atcaggaatg ccattatgag
     1381 cctgattatt gatgttattt cgcgtaaaac atccgtcaaa caaacgctga ttaatcctgg
     1441 cgacgtcacg gttgttattt atgagccttc cgtggtgcag gttcatgctc aggcctctgc
     1501 cgttgcgcgt tacgtccgtg aaggaaatga cctgctgatc tatatgcagg acggcacggt
     1561 gatccgctgc aacggttatt tcctgcaagc ggcgaataca gctgaacaat cggaactggt
     1621 gtttgccgat ggtcaacagc taacccatat cacctttgcc gatactgctg cgggtggatt
     1681 agcccccgta gaactgactg cccagaccac tgcgattgaa agcattgcgc catttcttga
     1741 taccgttgct cagaccagcg ccttcccgtg gggttggctg gcgggggcgg cggtaggtgg
     1801 tggcgcgctt ggtgcactgc tggcaagcgg tggcgatggc gactcgaaaa cagaagtgat
     1861 taataaccct acgccacctg ctgagcctgg caacgccaca ccatcatttt tagttaccga
     1921 taatcagggc gatcagcgcg gcattctagc caccaatgac atcaccgatg acaccacgcc
     1981 aacctttagc ggcagcgggc aggcgggggc gactattcag attaaagaca gtaacggcaa
     2041 tactattgcc agtactcagg tagacaacaa cggtcactgg agtgtctcgt tacccacgca
     2101 aagtgcaggt gaacatacct ggtcagtggt gcaaattgtc ggcagtacca tcactgacgc
     2161 cggttcgata acgttaacca tcgacaatag tcaggccagc gtgcaggttg ccaccaccgc
     2221 aggcgataac attattaacg ccagcgaaca ggccgccggg tttacgcttt ctggcaccag
     2281 tagccatctg gcgcagggaa cagaactcac cgttacgcta aacggcaaaa cctacacgac
     2341 cagcgtaggc gctaacggtg cctggagcgt gcaggtgccg accgccgatg cacaggcgtt
     2401 aggcgaaggg aatcaggcgg tgctggtcag tgggaaagac gccacaggca atacggtcac
     2461 cggcgcgcag ctactaacgg tcgataccca accgccaacg cttgccatca acaccatcgc
     2521 tcaggacaac attatcagtg ctgcggaaca taacgtcgcg ctggtactga gcggcacgtc
     2581 gaatgcagaa gcggggcaaa ccgtaacact gaccgtcaac gggaaaagcc atacagcaac
     2641 cgtcggtagc gacggaacct ggcaagtgac gctgcctgcc acggaagtcc aggcactggc
     2701 ggagggtaat tacgctgtca atgccagtgt cagcgatcgg gcagggaaca ccaccagcca
     2761 cagcgcgaat ttcacggtag acacctcagc acccgtggtc agtgttaata ccgtggcggg
     2821 cgacgatatt cttaataatg ccgagcaggc cgtcgcgcag atcatctccg gacaagtcag
     2881 cggtgcttct ccaggcgata cggtaacggt gaaattgggc actcatgtcc tgacgggcat
     2941 cgtgctggca gatggcagct ggaatgtggc gctggaccca gcggtaaccc gcacgctgga
     3001 tcgcggagcc aatacgattt tcgtcaccgt gacagatgct gcaggaaata ctggcgcggc
     3061 gtctcgagca atcacgctgg tcggtgtttc tccgttgatc accattaaca ccgtctccgg
     3121 cgatgacatt atcagtggcg cagaaaaagg tgcgccactg acccttaccg gtagcactca
     3181 acaggctgag acaggacaaa ccgtcacagt aaccctggct ggacagagtt ttaccactac
     3241 cgtgcaggcc gatggctcct ggagtctgac ggtacctgcc gccgcgatgg gaaatctgcc
     3301 tgacggcgcg gtggcgatta ccgcttctgt gacggatctc agcggcaata ccggcaacac
     3361 ttcccgcacc attaccgtcg atagccaggc cccggcctta agcattgatc cactgaccgc
     3421 tgataacatc attaacgccg ccgaaagcgg gcaggatctg cccatcaccg gcaccaccga
     3481 cgctcagccg gggcagacgg tgaccgttac gttaaatggg cagacgtatc agggcgtcgt
     3541 gcagccagac ggcacctgga gcgtgactgt gcccgccgcc aacgtgggcg cactggctga
     3601 cggcaacgct acggtcaccg ccagcgtgaa cgatgtcgcc ggtaatccga gcagcgtttc
     3661 acgcgtggcg ctggtggatg ccacgccgcc ggtggtaacc attaatccgg tggcgaccga
     3721 taacgtcatc aacacgccgg aacatgctca ggcgcaaatc atcagcggca cggttactgg
     3781 cgctcaggcg ggcgatatcg tcaccgtgac gctgaataat gtggattaca ccacggtggt
     3841 ggatggttcc ggcaactgga gtctgggcgt tccggcctcg gtggtcagtg ggctggcgga
     3901 cggcagttat cctgtcagcg tctcggtaac cgacaaagcc ggaaacacgg gcagccagtc
     3961 attgaccgtc acggtcaata ccgccgcgcc ccttatcggc attaacagca ttgcgggcga
     4021 tgatgtgatt aacgccagcg aaaaaggggc cgatctccag attaccggca ccagcgatca
     4081 gcctgttaac accgccatca ccgtgacgct gaacgggcaa aattacacca ccacgaccga
     4141 cgcctccggc aactggagcg tcaccgttcc ggcatcggcg gttacagcat taggccaggc
     4201 caactatacg gtaacggcgg cggtgaccag cgatatcggc aacagcgcca ctgccagcca
     4261 taacgtgctg gtcgacagcg cgctgcccgg tgtgaccatt aatccggtgg caaccgacga
     4321 tattattaac gccgccgaag cgggcgtggc gcaaaccatc agcgggcagg tgactggcgc
     4381 ggaagatggc gacacggtaa ctattacgtt gggtggtaat acttatacgg cgacggtggg
     4441 cagcaatctc acctggagcg tggacgttcc agcggcagat attcaggcgc tgggaaatgg
     4501 cgattttacg gttaatgcct cagtcaccaa tcaaaacggc aacaccggca gcggcacgcg
     4561 ggatatcacc atcgacgcca atctgcccgg cctgcgggtc gatacggtgg cgggcgatga
     4621 tgtggtcaat atcatcgagc acgggcaggc gctggtggtc accggcagca gctcggggct
     4681 ggctgaaagc acgccgctta ccgttacgat taataatgtg gaatacacca ctgcggtgca
     4741 ggccgatggt agctggagcg tgggcgtcac ggcggcgcag gttagcgcct ggcctgcggg
     4801 gacggttaat attgccgttt caggggaaag tagcgccgga aactcggtga gcattacgca
     4861 tccggtgacg gtggatctca ctccggcagc gatcaccatc aacaccatcg ccacggacga
     4921 tgtgattaac gccgcagaaa aaggcgctga tttaaccctt tccggcacca ccactaacgt
     4981 agaacccggt caaaccgtca ccgtcacctt tggcgggaaa aattacactg ccagcgtagc
     5041 gagcgatggt agctggactg ccaccgtacc cgccgccgat ctggcgtcat tacccgaggg
     5101 cagcgcctcc gcactggcca gcgtcagcaa tatcaacggc aatagcgcct cggcggtgca
     5161 caactacagc gtcgacagca gcgcgccaac catcattatc aataccgtcg ccagcgacaa
     5221 tatcgtcaac gccagcgaag ccgatgcggg cgtgacggtg agcggcagta ccaccgccga
     5281 agcggggcag attgttacga taacgcttaa cagcccgacc gtgcagacct atcaggcaac
     5341 ggtgcaggcg gacggcagct ggagcatcaa tattccggcg gcagatcttg aggcattgac
     5401 cgatggcagc cacaccctga ccgccacggt caatgacaaa gcgggcaatc cggcgagcac
     5461 cacgcataat ctggcggtgg atctcaccgt tccggtgctg accatcaaca ccattgcggg
     5521 cgatgacatt attaacgcca ccgaacacgg gcaggcgctg gtgatttccg gttccagcac
     5581 cggcggagaa gcgggggatg tcgtcaccgt cacgctaaac agtaaaacct acaccaccac
     5641 cctggacgcc tccggcaact ggagcgtcgg cgttccggcg gcggatgtca cggcgcttgg
     5701 cagcggcccg caaactgtca ccgccacggt taccgatgcg gcaggcaaca gcgacaatta
     5761 gacacacacg gtcaccgtta acctcaccgc accaaccatt gacatcaaca ccatcgccac
     5821 cgatgacata attaacgcca gcgaaaaagg cgcggatctg caaatttccg gcaccagtaa
     5881 tcagccagca ggcaccacga ttacgctggc gcttaacggg caaaattaca ccgcgacgac
     5941 ggacgctgca ggcaactgga gcactacggt gccagcttcc gcagtaggtg cgctgggcga
     6001 agccagctac acggtgacgg cgaacgtcac cgacagcgca ggcaacagca attccgccag
     6061 ccataacgtg caggtcaata ccgcgctgcc tggcgtcacc attaacccag ttgcgaccga
     6121 cgatattatt aacgccgccg aatcgggcaa tgcgcaaacc atcagcggcc aggtgacggg
     6181 ggcggcggcg ggcgatacgg ttaccgtaac gcttggcggg aaaacttaca ccgctaccgt
     6241 gcaggggaat ttaagctgga gcgtggacgt tccggcggcg gatattcagg ccatcggcaa
     6301 tggcaatctg acggttaacg cttcggtgac caacggcgtt ggcaatactg gcagcggttc
     6361 gcgagatatt actatcgacg ccaacctgcc aggtctgcgc gttgacaccg tggcgggcga
     6421 tgatgtggtc aatagcatcg agcacgctca ggcgctggtg ataactggta gcagcagcgg
     6481 gctggcggcg ggcgcggcgc tgacggtggt gattaacacg gtcacttacg ctgcaacagt
     6541 attagccgat ggcacatgga gcgttggtgt tccggcggca gacgtgagta actggcctgc
     6601 gggtacggtg aatatcacgg taagtggcac taacacagcc ggaacaacat ccaccatcac
     6661 ccatccggtc accgtcgatc tggcggcggt ggcgatttcc attaacaccg tttccggcga
     6721 cgatgtgatt aacgccgccg aaaaaggggc agatttaacc ctttccggca gcacctccgg
     6781 cgtggaagtg gggcaaacgg tcaccgttac ctttggcggg aaaacctaca ccgctaccgt
     6841 agcgggcgat ggtagctgga caaccaccgt acccgccgcc gatctcagcg tgttacgcga
     6901 cggcgacgcc accgtgcagg ccagcgtcag cactattaac ggcaacacgg cttcggcaac
     6961 ccacgcctac agcgtcgatg ccacggcccc gacgcttgcc attaacacca tcgccaccga
     7021 cgatattctg aacgctgccg aggcgggcaa tccgttaacc atcagcggta gcagcaccgc
     7081 cgaagcgggg cagacggtaa ccgtcacgct taatggtgtg acttacagcg gctccgtcca
     7141 ggcggacggc agctggagcg tcagcttacc gacggcggat ctcagcaatc tgaccgccag
     7201 ccagtacacc gttagtgcct cggtaagcga taaagcgggt aacccggcgt ccgctaacca
     7261 cgggctggcg gtggatctca ccgtgccggt gctgaccatc aacaccgtct ccggcgatga
     7321 cattattaac gccgccgaac acggacaggc gctggtgatc tccggctcca gcactggcgg
     7381 cgaagcgggt gatgtcatca ccgtcacact aaacagtaaa acctacacca ccatgctgga
     7441 cgcttccggc aactggagcg tcggcgttcc ggcggctgac gtcactgcgc ttggcagcgg
     7501 cccgcaaact atcactgcgg caattaccga cgcggcaggc aacagcgatg acgccagccg
     7561 cacggtcacc gtgaatctcg ccgcgccaac cattggtatc aacaccatcg ccaccgatga
     7621 cgtgattaaa gccacggaaa aaggcgcgga cctgcaaatc accggcacca gtaatcagcc
     7681 tgcgggcacc accattacgg tgacgctgaa cgggcaaaat tacaccgcta ctaccgatag
     7741 caacggcaac tggagcgcca cggtgccagc gtcagcggtt agcgcattgg gtgaagccaa
     7801 ctacacggta acggcaaacg tcactgatac ggcaggcaac agtaattccg ccagtcataa
     7861 tgtgctggtc aacagcgcct tgcctgccgt taccattaac gcggtggcga ctgacgatat
     7921 tattaacgct gccgaatcgg gaaatgcgca aaccatcagc gggcaggtga cgggcgcagc
     7981 gcagggggat acggttaccg ttacgctggg cggcaacacc tacaccgcca cggtgcagtc
     8041 taatttaagc tggagtgtgg acgttccggc ggcggatatt caggcgctgg gcaacggcga
     8101 cctgacggtt aatgcctcgg tcaccaatgg cgtcggcaac accggcagcg gctcgcgcga
     8161 tatcacgatc gacgccaatc tgcctggcct gcgggtcgat accgtggcgg gcgatgatgt
     8221 tattaacagc attgagcaca atcaggcgct ggtgatcacc ggcagcagca gcggattaac
     8281 ggcgggaacg gcgttaacgg tcgagattaa caacgttact tatggcgcga cggtattagc
     8341 cgacggcacg tggagcctcg gtgttccggc ggtagacgtc agcaactggc cagcgggtac
     8401 ggtgaatatc acggtaagcg gcaccaacag tgccggaaca acctccacca ttacccatcc
     8461 ggttaccgtc gatctggctg gggtcgccat caccattaac actctctctg gtgatgatgt
     8521 gatcaacgcc gtcgaaaaag gcgaaacgct ggtcgtaagc ggcagcacca gcggtgtcga
     8581 agccgggcag acggtgaccg tcacctttgg cggcaaaaat tacaccacca cagtggaagc
     8641 taacggtagc tggacggtga atgtgccgcc tgccgatctc gctgcgctac cggacggcgc
     8701 gggcaacgtg caggcgagtg tcagtaatat taacggcaac agcgcccagg ccgatcgcgc
     8761 gtatagcgtt gatgccaccg cgccgcttgt gaccatcaac accatcgcca gcgacgatat
     8821 ccttaacgtg agcgaagctg gcgcggggat caccatcagc ggcactacca cggcgcaggc
     8881 cgggcagacg ctcaccgtca cgctcaataa caacacgtac cagaccaccg ttctggcgga
     8941 tggcacctgg agcgtgaatg ttccggcagc ggatttaagc ggattaaccg ccagcagtta
     9001 caccgtgacc gccacggtga gcgacaaagc gggtaacccg gcaagcgccg accacgcgct
     9061 ggtggtagat atcactgcgc cggatctcac cattaacacc gtcgcgggcg atgacattat
     9121 caacgccatc gaacatggtc aggcgttggt ggtcagcggc accagtacgg gcgcggcggc
     9181 gggtgatgtg gtaaccgtca cgctgaacgg taaaaactac accaccacgc tggatgcctc
     9241 cggtaactgg agcgtgggca ttccggcggc ggatgtcacg gcgctggcga ccggtagcca
     9301 gaccatcacc gccagcctga gcgatcgcgc gggcaacagc gacagcacga ctcacgatgt
     9361 gaccgttgat cttagcggcc cgacgctgac cattaacacc gtctccggcg atgacattat
     9421 caacgccgct gaaatcgttg tggcgcagac catcagcggt caggtcacgg gaacggcggt
     9481 tgccgggaat acggtgattg tcaccattgg cggcaatcaa tataacgcca ccgtgcagtc
     9541 agatttaagc tggagcgtca gcgtaccggc gaacgttttg caggcgctgg gtaacggtga
     9601 actgaccatc agcgcctcgt tgaccaattc cgcaaataat accggcaccg cgacgcacga
     9661 tatcgtgata gatgccaacc tgccaggtct gcgcgtcgat accgtggcag gcgatgatgt
     9721 gattaacagc atcgagcaca ctcaggcgtt ggtgatcacc ggtagcagta gcggactggc
     9781 ggcgggcgcg gcgttgacgg tggttatcaa tagcgtcacc tacggcgcaa cggttttagc
     9841 ggatggtagc tggagcgttg gtgttccggt ggcagatgtc acaaactggc ctgcggggac
     9901 ggtcaatatt gccgtctctg gcaccaacac cgccggaacc acaaccagca ttagccatcc
     9961 ggtcacggtc gatctcgctg ccgtggcgat caccatcaac accctttcca ctgacgatgt
    10021 gattaacgcc gccgagaaag gctctgattt gcagctctcc ggcaccacct ccggcgtgga
    10081 agcggggcaa accatcaccg ttatcttcgg cggcaaaagc tacaccacca cggttgccgc
    10141 ggataatacc tgggggctga cgatccctgc cgtcgatgtt gcaaccttgc cagacggcgc
    10201 agcaaacgtc caggccagcg tcagcaatgt cgcagggaac agcacccagg caacgcatgc
    10261 ttacagcgtt gatgccactg cgccctccgt taccatcaac accatcgcca cggacgatat
    10321 tcttaacgcc gccgaagcgg gatcggcgct gaccatcagc ggcaccagca cggcggaagc
    10381 cgggcagacg gtgaccgtaa cgctaaacgg cgttaattac agcggcaatg tgcaggcaga
    10441 cgggagctgg agcgtcagcg taccaaccgg cgatttagct agccttaccg ccagctcgta
    10501 taccgttaac gcctcggtca gcgacaaagc cagaaattcg gcttcggcaa cgcataatct
    10561 gacggtggac cttgccgctc cggtcgtcac catcaacacg gtggcgggcg atgacatcat
    10621 taacgccacg gaacacggac aggcgcagat catcagcggc tcggcaacgg gcgcgactac
    10681 cggtaatacg gtttccgtga cgattggcac gaccacctat accaccgtgc tggacgccaa
    10741 cggcaactgg agcatcggcg tgcctgccag cgtgatttcc gcgctggcgc agggcgatgt
    10801 gaccattacc gctacggtca ccgactccgc aggcaacagc ggcacggcct cgcacactgt
    10861 caccgtggcg ctcggcgctc cggtgctcgc cattaacacc attgccgtcg atgacatcat
    10921 caacgccgcg gagaaaggcg cggatctggc gattaccggc accagcaacc agcctgcggg
    10981 cacgcagatt accgttacgc tcaacgggca aaattacacc accactgccg atgcttccgg
    11041 taactggagc gtgaccgttc cggcgtcacg ggtgagcgcc ctcggtgaag ccacctacac
    11101 ggtgaccgca gccgccactg acgccgatgg caacagcggt tccgccagcc ataacgtaca
    11161 ggttaatacc gcgctgccgg gcgtcaccat taacgtggtg gcaacggacg atattattaa
    11221 cgccgccgaa gcgggcgtgg aacagaccat cagcgggcag gtgacgggtg cggcggcagg
    11281 cgacacggtg accgtcacgc tcggcggggc gacttacact gccacggtgc aggcgaattt
    11341 aagctggagc gtcgatgttc cggcctccgc gctacaggag ttgggcaacg gcgaactgac
    11401 catttcggct tcggtgacga acagcgtagg caatactggt aacggcacgc gcgaaatcac
    11461 catagacgcg aatctccccg gtctgcgggt cgacaccgtg gcgggcgatg atgtggttaa
    11521 tattatcgag cacgggcagg cgctggtgat taccggcagc agctccggtc tggcggcggg
    11581 cagcaacgtc acgctgacca ttaacgggca aacctatgtt gcggcggtgc tggcggatgg
    11641 cacctggagc gtcggcgttc cggcggtgga tgtcagcgcc tggcctgcgg gatcggtgac
    11701 gattgcggcg agcggtagca cctctgccgg aaatccggta agcgttacgc atccggtgac
    11761 ggtcgatctc tcggcggtgg cggtgagcat caacgccatt accgccgatg atgtgatcaa
    11821 cgctgccgaa aaaggcgcgg cgttaacgct ctccggcagc acctctggcg ttgaagccgg
    11881 acaaacggtt accgtcacct ttggcggcaa aacttacagc gccacggtgg ctgcgaatgg
    11941 ttcctggagc acctcggttc cggcggcaga tatggcggct ctgcgtgatg gcgatgccag
    12001 cgcacaggcc agcgtcagca atgttaacgg caacagcgcc accacgaccc acgcttacag
    12061 cgttgatgcc agcgcgccaa cggtgaccat taataccatt gcgggcgatg atattcttaa
    12121 cgccgccgaa gccggagcgg ctctgaccat caccggcagc agcacggcgg aagcggggca
    12181 gacggtgacc gtcacgctca atggcacaaa ctacaccggc accgtacaga cggacggcag
    12241 ctggagcgtc agcgtaccgt cagccgactt aagcaccctg accgccagca actacaccgt
    12301 gaacgcggcg gtgagcgaca aagccggaaa cccggcctcg gttaatcaca acctgacggt
    12361 ggatacgtcc gttctggtcg tcaccatcaa cacggtggca ggcgatgatg tgatcaacgc
    12421 gacggaacac gcccaggcgc agatcatcag cggctccgcc actggagcgg caaccggtag
    12481 caccgtgacg gtgactatcg gcacaaatac ctttaccacg gtgctggatg ccagcggcaa
    12541 ctggagcgtc ggcgttccgg caagcgtcgt ctcggcactg gcgaatggca cggtgaccat
    12601 caatgccagc gtcaccgatg ccggaggaaa cagcggcagc gctacccatc aggtgacggt
    12661 caataccggg ctgccgacca ttacctttaa cgccatcagc ggcgataaca tcctgaacgc
    12721 cgatgaaaaa ggccagccgt tgaccatcag cggcggcagt acggggctgg cgacgggcgc
    12781 gcaggtcacc gtcacgctca acggtcacaa ctacagcgcc accaccgacg catcgggcaa
    12841 ctggacctta accgtgccgg tgagcgatct ggcggcatta ggtcaggcca actatacggt
    12901 cagcgccagc gccaccagtg cagcaggcaa caccgccagc agccaggcga atttactggt
    12961 cgacagcggc ctgccggacg tcaccatcaa caccgtggca ggcgacgata ttatcaacgc
    13021 cgccgaagcg ggggccgatc aaaccatcag cggggtggtg actcgcgccg ccgctggcga
    13081 tacggtcacc gtgacgctgg gcgggaacac ttacaccgct acggtacaga gcaacttaag
    13141 ctggagcgtc agcgttccga cagccgatct ccaggcgttg ggcaatggtg atttgaccat
    13201 taccgcctcg gtcaccaacg ctaatggcaa caccgggagc ggcacgcggg atatcaccat
    13261 tgatgccaac ctgccggggc tgcgcgtaga taccgtggcg ggcgatgata tcgtcaacag
    13321 catcgagcac gggcaggcgc tggtgatcac cggcggcagt agcggcctga atgcaggtgc
    13381 tgtgctgacg gttaccatca acagtgtggc gtattccgcc acggtgcagg cggacggaag
    13441 ctggagcgtt ggcattccgg cggcaaacgt cagcgcctgg cctgcggggc cgttaaccgt
    13501 ggaggtagac gggcaaagca gcgccaataa cccagtcagc gtcagccatc cgttcaccgt
    13561 cgatttaacg gcggtggcaa tcagcatcaa caccgttgcc tgcgacgacg tgattaacgc
    13621 cgcagaaaaa ggcaccaatc tgactctttc cggcagtacc agcgggattg agagcgggca
    13681 aaccgtcacc gtcacttttg gcggtaaaac ctacactgca agcgtcgccg ccaacgggag
    13741 ctggagtgta aacgttccgg cggcagatct ggcaactctg ccagagggcg cggcgaatgt
    13801 gcaggccagc gttagcagcg cgagcggtaa cagtgcctcg gcgacccatg cgtatagcgt
    13861 tgacgccagc gcgccgacgc tcaccattaa caccatcgcc agcgacgata tccttaacgc
    13921 cgcagaagcc ggaagcccgc tcaccatcag cggcaccagc accgccgaaa ccgggcagac
    13981 ggtgaccgtc acccttaacg gcgcaaccta caccggcact gtgcaggcgg acggtagctg
    14041 gagcgtcagc gttcccactt cagccctggg cgcgctcaac gcaagcaatt acaccgtcag
    14101 cgccacggtc aatgacaaag cgggcaaccc cggcagcgcc agccataatc tggcggtaga
    14161 caccaccgcg ccggttctca ccattaacac cgtggcgggc gatgacatca tcaacgatgc
    14221 cgaacatgcg caggcgctgg tgatctccgg caccagtagc ggcggggaag cgggcgatgt
    14281 ggtgagcgtt gtgctcaacg gcaaaaccta caccaccacc ctggatgcct ccggcaactg
    14341 gagcgttggc gttccggcgg cagatgttac ggcgctgggt agcggtgcgc agaccatcac
    14401 cgccagcgtc agcgatcggg caggcaacag cgacgacgcc agccgcaccg tgaccgtcag
    14461 cctcagcgcg ccagtgatta gcatcaacac catcgcgggc gatgatgtga tcaacgcgac
    14521 ggaaaaagga tctgatctgg cgctttctgg caccagcgat cagcctgcgg gtacggcgat
    14581 caccgtcacc ctgaacggac aaaactacag cgccaccacg gatgcctccg gcaactggag
    14641 tgttaccgtg cctgcctcgg cggtcagtgc gctgggcgaa gcgacctaca gcgtgacggc
    14701 gagcgtcacc aatgctcagg gtaacagcag caccgccagc cataacgtgc aggttaatac
    14761 cgcgctgccg ggcatcacca ttaatccggt ggcgacggac gatattatca acgcttcaga
    14821 ggcaggcagc gcgcaaacta tcagcggcca ggtaaccggt gcggcggcgg gcagcaccgt
    14881 caccgttgaa ctgggcggta aaacttacac cgccaccgtc caggctgatt taagctggaa
    14941 tgtgagcgta cctgccgccg actggcaggc tctgggtaac ggtgagttaa cggttaatgc
    15001 ctcggtgacc aacgccgttg gcaacaccgg tagcggcacg cgcgatatca ccatcgatgc
    15061 cagtctgcct ggcctgcggg tggataccgt cgcgggtgat gatgtggtca atatcatcga
    15121 acacgctcag gcgcaggtga tcaccggcag cagctccggc tttgccgcag gcacggcgtt
    15181 gacggtggtg attaacaacc aaacttacgc cgccacggtg ttggcgaacg gtagctggag
    15241 cgtcggcgtt cctgcgacgg atgtcagtaa ctggcctgcg ggaacgctga atattaccgt
    15301 tagcggggcg aacagtgccg gaacgcaaac cagcattacc catccgctga ctgttgatct
    15361 caccgccgtc gccatcagca tgaacagcat caccagcgat gatgcgatta acgccgccga
    15421 aaaaggcgcg gcgttaacgc tctccggcag cacgtccggc gtcgaagcgg ggcaaaccgt
    15481 caccgtcacc tttggcggca aaacctacac caccacggtg gcggcaaacg gtagctggag
    15541 caccaccgtt ccggcggcgg atttggcggc cctgcgtgat ggcgatgcca gcgcccaggt
    15601 gcgggtgact aacgtcaacg gcaacagcgc cacagcaacg cacgaataca gcgtcgatag
    15661 cgccgcgcca acggtgacca tcaacaccat tgccagcgat aacatcatca acgccagcga
    15721 agcggcggcg ggcgtaacgg tgtccggcac cagcaccgcg caaaccgggc agacgctcac
    15781 cgtcacgctt aacggcacta actaccagac cacggtgcag acagacggca gctggagttt
    15841 aacgctgccc gccagcgact taaccgcact tgctaataac ggctacaccc tgaccgccac
    15901 ggtcagcgat ctggcgggta accttggcag cgccagcaaa ggcgtgaccg tcgataccac
    15961 tgcgccggtg atcagtttta acaccgtggc gggcgatgat gtgattaaca acgtcgaaca
    16021 cattcaggcg cagattatca gcggcaccgc cacgggcgcg gtggcgggcg accgcctggt
    16081 ggtgaccatc gccgggcagc agtatgtcac cagcaccgat gccagcggca actggagcgt
    16141 cggcgtgcct gccagcgtga tttccggcct tgcggatggc acggtgacca tcagcgccac
    16201 cattaccgat agcgcaggca acagcagcac gcagacgcac aacgtacagg tgaacacggc
    16261 ggcagtgtcg ctctcggtca gcactatcag cggcgataac cttattaacg ccgccgaagc
    16321 gggcagtgcg ctgaccctga gcggcactgg cactaatttc gcgacaggta cagtggtgac
    16381 cgtgttgctt aacggcaaag gctacagcgc caccattcag agcaacggga gctggagcgt
    16441 gaacgtgcct gcggcggatg ttgcggcact cagtgatggc accagctaca cggttagcgc
    16501 ctccgctcag gacagtgccg gaaacagcgc cacggcctcg cgcagcgtgg cggtggatct
    16561 caccgcgccg gttatcagca ttaacaccgt ttcgacggac gaccgcctca acgccgccga
    16621 acagcagcag ccgttaaccc ttaacggctc gaccagcgcg gaagtggggc agacggtcac
    16681 cgtcaccttt ggcggcaaaa cctataccgc cacggttgcc gccaatggta cctgggcgct
    16741 gaacgtgcct gcggtggatc tggccgcgct cgggcagggc gcgcagacca ttaccgccag
    16801 cgtgaacgat cgcgccggta accccggaca ggccacgcac gccctgaccg tcgataccgt
    16861 tgcgccaacg gtcaccatcg ccacggtggc gggtgacgat attatcaaca acgccgagca
    16921 gcttgccggg cagaccatta gcggcaccac caccgccgaa gtgggccaga cggtgaccgt
    16981 tacctttaac gggcaaacct ggagcgcaac ggttggcagc ggcggaagct ggtcggtgtt
    17041 tattccggcg cagcagtttg ccggattaag cgacggcagc tacaccatta gcgcgacggt
    17101 tagcgatcag gccggaaacc ccggcagcgc cagccgtggc gtgacgctga acggcgatgt
    17161 acctactgtc accatcaaca cctttgcggg cgacgatgtg gtgaatgcag cggaacacgg
    17221 ctcatcgctg gtgatcagcg gcaccaccac cgcgcccgtc gggcagacgc tgaccctgac
    17281 cttaaacggc aaaacctaca ccaccacggt gcagacgggc ggtagctgga gctacaccct
    17341 cggcagcgcc gatgtcaccg cgctggcgga cggcaacgcc tacgtgatta acgcctcggt
    17401 gagcaatgcc attggcaaca ccggtagcag taatcacacc attaccgtcg atctcagcgc
    17461 tccggcgatg ggcattaata tcgattccct gcaagccgac actggcctta gcgccagcga
    17521 ctttatcacc agcgtcagtc cggtagtggt caacggctcg ctcaccgccg cgcttgccag
    17581 taacgagacg gcgcaaataa gtatcgacgg cggcacaacc tggaccacgc ttaccgttac
    17641 cggcacgacc tggcgctata acgacagccg cacgctgacc gatggcaact atctctatca
    17701 ggtgcgggtg attgacgcag cgggcaacgt tggcgcgacc gacagccaga atgtggtgat
    17761 cgacactacc gcgccagatc ccgcggtgaa aaccatcgcc atcagcgcga tcaccaccga
    17821 tatgggcttg atcactaacg attttgtcac cagcgacacg acgcttgcgg tgagcggcac
    17881 gctgggggcg acgctttctg ccggtgagtt cgcgcaaatc agcctcgacg gcggcgtcac
    17941 ctggactacg ttaaccgtcg ttggcaccag ctggagctat gccgatggtc acacgcttac
    18001 tgacggcacc tggaactaca cggtgcgggt ggtggatctg gcggggaacg ttgggcagac
    18061 cgcaacgcaa aacgtggtgg tcgacaccac cagcccggaa gcggcgaaaa gtatcaccat
    18121 taccggtatc agcgatgaca ccggaaccag cagcagcgat tttattacca gcgacaccac
    18181 gcttaccgtg cgcggcgtat tgggcgcggc gctcggcgct aatgagttcg cgcaaatcag
    18241 taccgacaac ggcgcaacct gggtgaacgt gaccgtcgcc gcagacagcc tgaactggag
    18301 ttacgttgac ggacgaaccc tcaccaacgg caccaccacc tggcaggtgc gggtggtcga
    18361 tctggcgggc aacgttggcg caacgagcag ccagtcggcg ctgatcgata ccgttaaccc
    18421 ggcgcaggtg ctcaccatcg ccagcatcag caccgacacg gggagttcgg caactgactt
    18481 tatcaccagc gacaccatgc tcacgctgac cggttcgctg ggggcggggc ttgccagcgg
    18541 cgaagtggcg cagattagcc ttgatagcgg cgcgacctgg acaacgctca ccaccaacgg
    18601 tacacagtgg acttacaccg acagccgcac gctgaccgac ggcagctacg tttatcaggt
    18661 gcgggtgctg gatctggcgg ggaacaccgg cccggtggtg tcgaaaacgg tggtggtcga
    18721 tacgattaac cccaccgcca caccaacgat tgtgtcgtat accgatgatg tcgggcagcg
    18781 gcaggggaca ttaagcagtt cgcaggccac cgacgacact acgccgctgc tgaacggtgt
    18841 actttccgcg ccgcttgcca gcggtgaagt ggtttacctc taccgtaacg ggctgctgtt
    18901 aggggcggtg acgatggtcg gcgctctgaa ctggacctac agcgacagcg ggctggtgag
    18961 cggtgcctat acctacagcg cgcgagtggt ggatttggcg gggaatatca cctcctccag
    19021 tgattttgtc ctgacggtcg atacctctat tccgaccacg ctggcgcaaa tcaccagcca
    19081 gaccacgcgc gatactacgc cgattattag cggggtgatc accgccgcgc ttgccagtgg
    19141 gcagtatgtt gaagtggtga tcaacggcaa aacctacacc tctgaaccgg gcggcgcggt
    19201 agtggtcgat ccggcgcaca acacctggta tgtacagttg ccggataccg atgcgctgac
    19261 agtttccgcg accgcctata ccgttactgc gcaggtaaaa agttcggcgg gtaacggcaa
    19321 taacgccaat attagcaacg gcacggtgac ggttaacgcg gcgattgatt acacaccgac
    19381 ctggactacc gccagcaaaa ccaccgcctg ggggctgacc tacggcctcg actcgcacgg
    19441 gatgtggacg gtgctggcaa accagcaggt aatgcaatcg actgacccac tcacctggtc
    19501 gaagaccgcg ctgacgctgt atcagagcgg caacaactac gccaccagct ccattgccga
    19561 ttacgaccgt aacggcacgg gcgatctgtt tatcacccgt gatgactacg gtacgggcta
    19621 tattaacggc tttaccaata acggcgatgg caccttttcc agcgctattc aggtcaccgt
    19681 cggcaccctg acgtggtacg gctcgattgt ggcatttgat aaagagggcg acggctatct
    19741 cgacttctgg attggtgacg ctggcgggcc ggactccaac accttcctgt ggaacaacgc
    19801 aggcacgctg gtaggcaact ccaccacgtc gaacagcggc ggtagcgcca cggtgggcgg
    19861 ggcggtgacg gggtatcttt cgctcaacga aggttctggc gtcgatctga acaatgacgg
    19921 caggatcgac ctggttcagc acacctataa cctgaacaac tattacacgc tgtcttcgct
    19981 catcaaccag gggaatggga cgtttgtctg ggggcagaac accaccaata ccttcctgag
    20041 cggggcgggc agtggcgcta tgagcagcag cgtttccatg acctgggccg atttcgatgg
    20101 tgacggcgat atggatctct tcctgcccgc cagccaggga agagctaact acggctcgct
    20161 gttattcaac accaacggcg tactgggtag cccggtggcg gtgggcgcaa cggcaaccac
    20221 ctacgccagc cagtttagcc tggcggtgga ctggaaccac gacggcctga tggatatcgc
    20281 ccgtatcgcc cagaccgggc agtcgtatct ttatactaac gtcagcaacg ccagcaactg
    20341 gacgcaatcg gccctcggcg gcagccagag cggtaccacc agcggcgtgg cggcaatgga
    20401 ctacgactgg gacggcgcgg tggatgtgct ggtgtccaaa cagtcgggca gcgtgttcct
    20461 gagccgcaac accaacacgg tgagctacgg cacttcgcta cacctgcgca tcaccgatcc
    20521 caacggcatt aacgtctatt acggcaatac cgtgaagctg tacaactcgg cgggagtgct
    20581 ggtcgccacg caaatcatca acccgcagtc gggtatgggg gttaacgaca cctcggcgct
    20641 ggtcaacttc tacgggctga atgccggaga aacctacaac gcggtgctga tcaaatccac
    20701 cggcaccacc gccagcaata tcgaccagac ggtcaacacc agctggggcg gtttacaggc
    20761 caccgatgcc actcccgctt acgatctcag cgctgaagcg ggtaccgcga gcaacaacgg
    20821 caagttcgtc ggcaccggct ataacgacac cttcttcgcc accgcaggca ccgacactta
    20881 cgacggttcc ggcggctggg tgtacagctc cggcaccgga acgtggctgg cgaacggcgg
    20941 gatggacgtg gttgatttcc ggctttcgac ggtgggcgtg acggctaact taagcagcac
    21001 tgccgcgcag gccaccggtt ttaacacctc gacatttacc aatatcgaag gcatttccgg
    21061 ctcgaatttt aacgacattc tgaccggcag cagcggcgat aaccaactgg aagggcgcgg
    21121 cggcaacgac acgctcaaca tcggcaacgg cggccacgac accttgctct ataaactgct
    21181 caacgccagc gacgccactg gcggcaacgg ctcagacgtg gtgaatggct ttacggtagg
    21241 gacatgggaa ggcaccgccg acaccgatcg cattgatatt cgtgaactgt tacagggcag
    21301 cggctacacc ggcaacggca aagccagcta cgtcaacggc gtggcaacgc tggatgcgca
    21361 ggccggaaac atcggtgact ttgtcaaagt cacccagagc ggcagcgaca ccatcgtgca
    21421 gatcgaccgc gacggcacgg gcggcacttt tgcgacaact aacgtggtca cgctgacggg
    21481 cgtgcacacc gacctcgcca ccttgctggc gaatcatcag ttgatggtgg tgtagataat
    21541 gaaaaaaaat gcccagtccg tggaagcctg gctggaggcg atgatcgccg tggcgcgcta
    21601 ttatcggctc gatttttctc aggagaatgt gcgcgcgacc gtcaactggg agcgcgacag
    21661 taagcgggag gagttactca ccgacatggc gcggcagtta gggatggggt tgcggttggt
    21721 ggagttttcc gccgattcgc ttaacccctg gcgtctgccg ctgatcgcgg tgtttgataa
    21781 tcagcagatt ggcgtgatca cccgtcgcga caatcatgac aatatcagcg ttcagttcag
    21841 cggtgacgaa gggctggaaa ctacacttaa cgtcgcggat attgaagata aaatcgtcga
    21901 actggcgctg ctgcgccctc tcagcgccat ccctgacgcg cgggtggatg actatattcg
    21961 cccgtatcag gcgaactggt tctggagtct ttcgctaaaa gactggcggc gctacggcga
    22021 tattatgctg gcgtcactgg tggcgaacgt gctggcgctg gcggcgatga ttttctcgat
    22081 gcaggtttac gatcgggtgg ttccggcgca atcgtacccg acgctatggg tgcttttcgc
    22141 cggggtgatg atggcgatcc tgtttgagtt ctgtatgcgc atggtgcgta cgcatctatc
    22201 tgatgtgatc ggaaagcggg cagacctgcg tatttcggat cgcgtttttg gtcatgcgct
    22261 acggctgaaa aacaacgtgc gatcgaaatc caccggatcg tttatctcgc agatccgcga
    22321 actggaatca gtgcgggagc ttattacgtc caccaccatc ggcgcggtgg cggacctgcc
    22381 attcttcctg ctgtttgtct ttattttgtg gatgatcggc ggctggctgg tgctggtggt
    22441 tttgctggcg ctgccgttgc tggtgatccc cggcctgctg gtgcaacgcc cgctggcgcg
    22501 gctggcgaac gaaggaatgc gcgagtcagc ggtacgcaac gctacgctgg tggaagccgt
    22561 gcagtcgatt gaagatatta aactgctgcg cgccgaacag cgttttcaga atcagtggaa
    22621 ccacaccaac gacgtggcct cgtccatcag catgaagcaa cgttttctca ccgggctgct
    22681 gctcacctgg acccaggaag tgcagtccat cgtctacgtg gtggtgctgc tggtgggctg
    22741 ctttatggtg atgaacggcg acatgaccac cggcgcgctg gtcgggacga cgatcctggc
    22801 ttcgcgcacc attgcgccgc tgtcgcagat atccggcgtg ctctcgcgct ggcagcaggc
    22861 gaaagtagca cgtaacggcc ttgatgaact gatgaagcgc ccggtggatc agccggaaca
    22921 cggcaaactg gtgcataaag cggtgctgca cggtaattat cagttcagca acgcggtgtt
    22981 ttattacgac gaagaagaga agattgccga tgtagcgatt ggcaaactca acattcaggc
    23041 tggtgagaaa atcgccattc tcgggcgcaa cggcgcgggt aaatcgacgc tactgcaaat
    23101 gctcgccgga atgcgtatcg cccagcaggg gcaggtgttg ctggataaca tcagtatcgg
    23161 gcagcttgat ccggcagatc tgcggcgaga catggggcta ctcagccaga ccgggcgact
    23221 gttctttggt tcgctgcggg aaaacctcac catggggatg ccggaagcca gcgatgaaga
    23281 tatcgaacgc gcgttaaccc tcagcggagc gctgcccttt gtgcagaagc agaaaaacgg
    23341 cctcaactac atgattcagg aagggggatt tggcctctct ggtggtcagc ggcaaacgct
    23401 gctgctggcg cggctactca tctctcagcc gaatatcgtc ctgctcgacg aacccagcgc
    23461 ctcactggac gaaatggcag aagcgtattt aatcgaacaa ctgaaacagt ggattgggca
    23521 tcgcacgctg attatcgcca ctcaccgcac ggcgatgctg caactggtgg ataggataat
    23581 cgtgatggat caggggcgga ttgtgatgga cggcgcgaaa gaggccattt tgcgtgaaca
    23641 gggcgagccc acagcgcgac gggtggtgtt gcaggagaag aataaggggt ctgcggcatg
    23701 aacggacaac tgcaatataa ccgtggtctg actgaacccc gcctgccgcg ttcggcgctg
    23761 gcggtacgcg ttaccgctgt gatgctgctc tgttttctcg gctgggcgtg gtacttccag
    23821 cttgatgaag tcaccaccgg cagcggcacg gtagaacctt ccgggcgcga acaggtggtg
    23881 cagtcgctgg aaggcggaat tctgtatcat ctcgacgtga aagttggcga tattgtcgaa
    23941 cagggccagc cgctggcgca gcttaaccgc accaaaacgg aatccgacgt ccaggaagcg
    24001 atgtcgcggc tgtacgcggc gctggcaacc tcggcgcggc tgcgggcgga agtgagcaat
    24061 aaaccgctgg tcttccctga cgaactgaat aaattccccg aacttatcga gtcggaaacg
    24121 gcgttgtaca acactcgccg cgacgggttg aacaaagcca ccaccgggct gacgcagggg
    24181 attagcctgg tcaatcgcga actggcgatg acccagccgc tggtaaaaca gggggcagcg
    24241 agcagcgtcg aagtactgcg cctgcaacgc caggccaatg aactggaaaa caaactcagc
    24301 gacgtgcgca cgcaatacta cgttcaggcg cgcgaagagt tagcaaaagc caatgcggaa
    24361 gtggaaaccc agcgttcggt gatccgtgga cgggaagact ctctcaccag gctcaacttt
    24421 accgcgccgg tgcgaggcat tgttcaggat attgatgtca ccacggttgg cggcgtcatc
    24481 gcccccggtg gtaaactgat gaccatcgtg ccgctggatg agcaactgct gattgaggca
    24541 aaaatctccc cgcgcgatgt ggcgtttatc cacccggggc aaaaatcgct ggtgaaaata
    24601 actgcctacg attactccat ctacggcggc ctgccgggag aagtggcggt gatatcacca
    24661 gacaccgtgc aggatgaagt gcggcgcgat gtttattact atcgggtgta cattcgcact
    24721 ttcagcaacc atctggaaaa caaaagcaaa cagcagtttc cgatattccc cggtatggtg
    24781 gcgacggtcg atatccgcac cggcaaaaaa agcgtgctgg attatctgtt gaaacccttt
    24841 aacaaagcac aggaggcgtt gcgcgaacga tgaacattag cgatgtagca aaaattaccg
    24901 gcctgaccag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    24961 gcagcgaaaa tggttatcga acctacacgc agcagcatct caacgaattg accttactgc
    25021 gccaggcgcg ccaggtgggc tttaacctgg aagagagcgg cgagctggtg aatctgttta
    25081 acgacccgca gcgtcacagt gccgacgtca aacgtcgcac gctggagaag gtggcggaga
    25141 ttgaacggca tatcgaagaa ctgcaatcta tgcgcaacca gctactggcg ctggcgaatg
    25201 cctgccccgg tgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    25261 atcatcgggc ggggtgaaaa tcagtgaatc cccgtttgca ccgaaagaat atgctgcaaa
    25321 ctttctacca taatgcgccc cgactgaaat aagccttcct ggatttcgtt gttaacattc
    25381 acagcgtgaa ccagggaggc taataatact acttgcaaga aggcaaaaat aaacgcaccg
    25441 ataatcataa tatgcgtcag gggtattttc ctttgctcct tcattttaaa taacccacaa
    25501 actggccttc tttcattaag gcgacttttc ctgtgtgatg ataaacaatg acgttatata
    25561 gacctgactg ataaacagtg gcatctttat tgtcttcatc aattgcacat aatgaatcat
    25621 tgagataagc aaagttttta ctgctcattc taaaggtcat gccaaatcct ttataaatgg
    25681 ctttctttgc cgcctgggcg tgttgtgcgg cttctgcatt tttaaatgcc tgtgcctcgg
    25741 atgcacttaa aatagccgcc tgacgctgct gttctgcgag gtaacaggtg tcatgactga
    25801 cgccctgcgc ctggcaatct gcgatgcggg aagtgggtga actacagcca gccagtaata
    25861 taaccgccag cagggaaaac gatttaatgg agctattcat cattgttaac cttaaataat
    25921 gtacaaatta atgaagaggt gaacccggca agcttccttg ctcttattta tattgaggat
    25981 tgaaatatta gaacgaatat cccacgccga cacctgcgcc gaaatcagat tgtgtatccg
    26041 cagaaatacc ggctttaagt accgtatgcg ttccggcatt aaatgatgtt ccgacagaca
    26101 ccgcagattc accgttgaag gttcccgctc ccgctgacac catcatcacg tcaccggttt
    26161 taacctgtgg ttgtgaggcg atagcgatgg cagatgcagt tcccgcattg gcttctttac
    26221 gattgtcatc aacttcgttt tttagcgaac tgaaatggtt attgagcgtg ttaatttgct
    26281 ggctgttatc tgcaacccgt ttttgggtgt tggttaactg ttggtgcgtt tgggttacgt
    26341 cttgtgacag gctgtcaatt tgttcatgtg cctgggcaga ctcctgcgcc agactttgca
    26401 tttgtttatc gtattgagtc ctttcatttt gctgttgtac tgccacatcg gttgtcagcg
    26461 cattcatttt ctgcccttgt tcagtggcgg tgcgctgatt agcctcattt tgagccgcca
    26521 gtctggcatt aatcatttcc tgataatgcg cggtattttg cgtatctcca gtcgcggcga
    26581 gcgttttttg tgtggctacg atctgcgcct gttgctctgt aatttgcgtt tgctgggcgt
    26641 cagttaccgc agactgtcgg gcgttgatca tgctctgata acgggcagtt gtctgtacat
    26701 cacctgtagc ggcaacggtt ttttgtcctg cgtcaggatc tgtctggaca gtgggagtct
    26761 gagtggtatc aggtgtttca gaagggatgt agtgatgttc agtcaaatag gtatacgcag
    26821 cggggctgtt gtctgtgcgt tctaaaaagc gggtaatctt ctgctcaatc tccgattgcg
    26881 acatgccaga gttaatggct gaaagctctg ttgccgccgc ttcaatggta tcaccagcta
    26941 aaacaacagg gctggatgtt gctgaaatta tgagtgccag taaagctacg tttacagttt
    27001 tcattcattt tctcttgttc aaaataagtc gtattaatgt ttctataaga aacaaataat
    27061 attgttttaa gaaataaact gcgttgcaat ttaatcgaaa tctacgccgc catttgtgtg
    27121 atgtaaagcg
//
LOCUS       DAZGXS010000057        27108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGXS010000057 DAZGXS010000000
VERSION     DAZGXS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHKG5_002486"
     CDS             complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHKG5_002486"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0868578.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHKG5_002487"
     CDS             complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHKG5_002487"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0868579.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4312..5112
                     /gene="nagB"
                     /locus_tag="ACHKG5_002488"
     CDS             4312..5112
                     /gene="nagB"
                     /locus_tag="ACHKG5_002488"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0868580.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5172..6320
                     /gene="nagA"
                     /locus_tag="ACHKG5_002489"
     CDS             5172..6320
                     /gene="nagA"
                     /locus_tag="ACHKG5_002489"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0868581.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6329..7549
                     /gene="nagC"
                     /locus_tag="ACHKG5_002490"
                     /gene_synonym="nagR"
     CDS             6329..7549
                     /gene="nagC"
                     /locus_tag="ACHKG5_002490"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0868582.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7597..8349
                     /gene="nagD"
                     /locus_tag="ACHKG5_002491"
     CDS             7597..8349
                     /gene="nagD"
                     /locus_tag="ACHKG5_002491"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0868583.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8606..10270
                     /gene="asnB"
                     /locus_tag="ACHKG5_002492"
     CDS             8606..10270
                     /gene="asnB"
                     /locus_tag="ACHKG5_002492"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0868584.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10650..10726
                     /locus_tag="ACHKG5_002493"
     tRNA            10650..10726
                     /locus_tag="ACHKG5_002493"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10684..10686,aa:Met,seq:cat)
     gene            10737..10821
                     /locus_tag="ACHKG5_002494"
     tRNA            10737..10821
                     /locus_tag="ACHKG5_002494"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10771..10773,aa:Leu,seq:tag)
     gene            10845..10919
                     /locus_tag="ACHKG5_002495"
     tRNA            10845..10919
                     /locus_tag="ACHKG5_002495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10877..10879,aa:Gln,seq:ttg)
     gene            10954..11028
                     /locus_tag="ACHKG5_002496"
     tRNA            10954..11028
                     /locus_tag="ACHKG5_002496"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10986..10988,aa:Gln,seq:ttg)
     gene            11044..11120
                     /locus_tag="ACHKG5_002497"
     tRNA            11044..11120
                     /locus_tag="ACHKG5_002497"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11078..11080,aa:Met,seq:cat)
     gene            11169..11243
                     /locus_tag="ACHKG5_002498"
     tRNA            11169..11243
                     /locus_tag="ACHKG5_002498"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11201..11203,aa:Gln,seq:ctg)
     gene            11281..11355
                     /locus_tag="ACHKG5_002499"
     tRNA            11281..11355
                     /locus_tag="ACHKG5_002499"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11313..11315,aa:Gln,seq:ctg)
     gene            complement(11509..12684)
                     /gene="ubiF"
                     /locus_tag="ACHKG5_002500"
     CDS             complement(11509..12684)
                     /gene="ubiF"
                     /locus_tag="ACHKG5_002500"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0868585.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12830..14254
                     /gene="miaB"
                     /locus_tag="ACHKG5_002501"
     CDS             12830..14254
                     /gene="miaB"
                     /locus_tag="ACHKG5_002501"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0868586.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14407..15447
                     /locus_tag="ACHKG5_002502"
     CDS             14407..15447
                     /locus_tag="ACHKG5_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0868587.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15444..15911
                     /gene="ybeY"
                     /locus_tag="ACHKG5_002503"
     CDS             15444..15911
                     /gene="ybeY"
                     /locus_tag="ACHKG5_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0868588.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16001..16879
                     /gene="corC"
                     /locus_tag="ACHKG5_002504"
     CDS             16001..16879
                     /gene="corC"
                     /locus_tag="ACHKG5_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0868589.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16904..18442
                     /gene="lnt"
                     /locus_tag="ACHKG5_002505"
     CDS             16904..18442
                     /gene="lnt"
                     /locus_tag="ACHKG5_002505"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0868590.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18839..19747
                     /gene="gltI"
                     /locus_tag="ACHKG5_002506"
     CDS             18839..19747
                     /gene="gltI"
                     /locus_tag="ACHKG5_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0868591.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19917..20657
                     /gene="gltJ"
                     /locus_tag="ACHKG5_002507"
     CDS             19917..20657
                     /gene="gltJ"
                     /locus_tag="ACHKG5_002507"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0868592.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20657..21331
                     /gene="gltK"
                     /locus_tag="ACHKG5_002508"
     CDS             20657..21331
                     /gene="gltK"
                     /locus_tag="ACHKG5_002508"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0868593.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21331..22056
                     /gene="gltL"
                     /locus_tag="ACHKG5_002509"
     CDS             21331..22056
                     /gene="gltL"
                     /locus_tag="ACHKG5_002509"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0868594.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22174..23109
                     /gene="rihA"
                     /locus_tag="ACHKG5_002510"
     CDS             22174..23109
                     /gene="rihA"
                     /locus_tag="ACHKG5_002510"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0868595.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23193..24863
                     /gene="hscC"
                     /locus_tag="ACHKG5_002511"
     CDS             23193..24863
                     /gene="hscC"
                     /locus_tag="ACHKG5_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP0868596.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALDDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24923..26374)
                     /gene="djlC"
                     /locus_tag="ACHKG5_002512"
                     /pseudo
     CDS             complement(24923..26374)
                     /gene="djlC"
                     /locus_tag="ACHKG5_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26371..27078)
                     /locus_tag="ACHKG5_002513"
     CDS             complement(26371..27078)
                     /locus_tag="ACHKG5_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0868597.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6654 a         7040 c         6885 g         6529 t
ORIGIN      
        1 gtgaacgcct tatccggcct acaaaagcac gcaaactcaa tatattgcag agatcatgta
       61 ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga aactgtttgt ctgttccttg
      121 ccggatgcga cgtaaacgcc ccatccggca tagcgaaact taaaattact cgcctacttt
      181 cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg ccgtagaatg
      241 gcggctatcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac ccgcaaccgc
      301 atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg ggttaatcac
      361 cgacaggaaa tcatccgcag cacctgggtt cggcacgctg aacagacggt catacaaacg
      421 gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt tgactttacg
      481 accatctgcc ggatctttgc ttaaggtatc ggcgtcataa gtacagaaga tggtggtgat
      541 attaccttcg gcatctttct cgacgcgttc tgccttaatc acataagcat tacgcagacg
      601 cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc ggaaatcgga
      661 gcgatcaatc caaatctcac cgctaaacgg cacctgacgg ctccccattt ccggtttgtt
      721 cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga taaccagttt
      781 caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt cacggatgca
      841 ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga tgcgtttgca
      901 gaactcacga atagaagccg cagtgtaacc acgacgacgc agaccggaaa tggtcggcat
      961 acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaagttca gcttacgctt
     1021 ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg ggtgaacagg
     1081 aatggtgatg ttgtccagta cccagtcgta cagacgacgg ttatcctgga actcaagcgt
     1141 acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga agtcgtacat
     1201 cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa tgcggtacag
     1261 caccggatcg cgcatcacga taaacggtga agccatgtcg attttcgcac gcaggcaggc
     1321 tttaccttct tcaaaaccgc cggtacgcat tttctcgaac agcgccaggt tctcttcaac
     1381 gctacggtcg cggtacgggc tgtttttacc aggctgcgtc agggtgccgc ggtattcgcg
     1441 gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca gttcgatcgc
     1501 ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag accagtgaaa
     1561 acctaaccac tcaacgtcgt ttttgatcga atcgacatac tcgatatctt cttttaccgg
     1621 gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga tcccgaagtt
     1681 caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg gcgggaaacg
     1741 ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct gacggataaa
     1801 gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta aacaacgtat
     1861 aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagttagtg aaaatcgaaa
     1921 atctcactga gattatgggg atgattgatg aaaaaaaaag cggcagagaa gatctccacc
     1981 gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag gcataaagca gattactttt
     2041 tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta atgatcaagc
     2101 cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt
     2161 tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc gcgccctctt
     2221 ccaccagacg tttaaagcct ttaccttcca gcgctacggt atcgataccc atgtgaacga
     2281 caatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg aagattttca
     2341 cgattgttcc tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt
     2401 cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc acgacatcac
     2461 cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg
     2521 ctacaggagc ggcagttgcc ggagttgctt cagcagaggc agctgctacc gggccacggg
     2581 caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca atcacctgaa
     2641 tagtctgttt gttcagtttt actaccccag aagcccccag acgtttacac atcgtatcgt
     2701 taacgcgggc agagtcagcc acggtaaggc gcagacgggt gatacaggcg tcaattgctt
     2761 tcaggttgtc agtgccgcca accgcagcaa tatagttggt cgccagttga ttgaggcctt
     2821 cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc ttctttatct tcacgacccg
     2881 gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga
     2941 agacaacacc cataaccagc agcatccaga cgttctggct ggcggccggc aggttataca
     3001 tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc ggcatggata cccagcagcg
     3061 ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa
     3121 ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa
     3181 cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg
     3241 ccagcgccgc acccggcagg ccgaacatca tgatcgggaa gaagccggac atgaacatcc
     3301 ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg
     3361 cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg
     3421 ttgggatcaa cagacggttg atgaaaccaa agataccgga acccagcgcg cccgcagaaa
     3481 cgatccactc gccgcctgca tggatagcgt gctgtaccgg cggccagacg taaccaaaaa
     3541 tggccgccag caccagacag aagaagccgg tggcgatcgg cacaaagcgt ttgccgccga
     3601 agaagctcag gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa
     3661 ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca
     3721 tcgctttggt taacacaaag taacctaccg cccccgccag cgccgccgca cctgcgctgt
     3781 ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg
     3841 caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca
     3901 acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga
     3961 aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc
     4021 cgtattttta ttgtgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca
     4081 tctgccacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgagt gtcttttgtg
     4141 agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaacc agaaaactta
     4201 ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct
     4261 taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg
     4321 atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt
     4381 atcaatgcgt tcaaaccgac tgctgatcgt ccgtttgttc tgggcctgcc aactggcggc
     4441 acgccaatga ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt
     4501 aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa
     4561 agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac
     4621 atcaaccttc tcaacggcaa cgccccggat atcgacgccg agtgccgcca gtatgaagaa
     4681 aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat
     4741 attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact
     4801 catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa
     4861 tatgccctga ctgtcggtgt tggtacactg ctggatgccg aagaagtgat gattctggtg
     4921 ctgggtagcc agaaagcact ggcgctgcag gccgcagttg aaggttgcgt gaaccatatg
     4981 tggaccatca gctgtctgca actgcatccg aaagccatca tggtgtgcga tgagccttcc
     5041 accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc
     5101 aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat
     5161 cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg
     5221 atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac
     5281 tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg
     5341 atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg
     5401 tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc
     5461 cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt
     5521 acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggcccg tggctgaatc
     5581 tggtgaaaaa aggtacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg
     5641 atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc
     5701 ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca
     5761 acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc
     5821 tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg
     5881 atgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca
     5941 ttcgtaatgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc
     6001 cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg
     6061 gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg
     6121 tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc
     6181 tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag
     6241 tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc gttaacggta
     6301 acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa
     6361 tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg
     6421 gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa
     6481 aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac
     6541 cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc aatttccacg caatcggcgt
     6601 acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc
     6661 agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg ccctgctgaa
     6721 tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga tcgccatttc
     6781 cgttatcctg ccaggacttg ttgacccgga cagcggcaaa attcattaca tgccgcatat
     6841 tcaggtagaa aactgggggc tggtagaagc actggaagag cgttttaaag tgacctgttt
     6901 cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga
     6961 ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa
     7021 cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata ttcaggtcga
     7081 accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgctgccaa
     7141 cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc
     7201 gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagcctggc
     7261 ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca ttgctatcaa
     7321 cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct
     7381 gctccctgct attgaaagct gcattaatac tcaggcgctg aaggcgtttc gtactaatct
     7441 gccggtggta cgttctgagc tagaccaccg ctcggcaatc ggtgcttttg cgctggtaaa
     7501 acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat
     7561 agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc
     7621 gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg
     7681 cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc
     7741 gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtacctga cagcgtgttt
     7801 tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcgtat
     7861 gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat
     7921 gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat
     7981 aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac
     8041 gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa aatctccggg
     8101 cgcaaaccgt tctatgttgg taagccgagt ccgtggatta tccgcgcggc attaaacaaa
     8161 atgcaggcac attcggaaga aacggtgatt gtcggcgata acctgcgtac cgatattctg
     8221 gccggcttcc aggcaggtct ggagacgatt ctggtgcttt ctggtgtttc gtcgctcgac
     8281 gatatcgaca gtatgccttt ccgccccagc tggatttacc cgtcagtcgc tgaaatcgac
     8341 gttatctgaa tagcaaccac gtctcaggac gtggtttctt atttttaccc atcattaaaa
     8401 tcatcccatt caataaaacc cgccaaaaat tatcgatcca tcaataaata catcaccgca
     8461 ccctcccctt tttcacaaat cagcaatcac cattacgttt tttatttttt caccgccaaa
     8521 tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac aacaagcaac
     8581 aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg atatcaaaac
     8641 agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg
     8701 cccggactgg tccggtattt atgccagcga taaagccatt ctcgctcacg aacgtctgtc
     8761 aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa cccacgtact
     8821 ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc gctgaatatg gcgatcgtta
     8881 tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc
     8941 ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga
     9001 tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg gctatgacga
     9061 acacggtcag ctgtatgtgg cctcagaaat gaaagccctg gtgccagttt gccgcacgat
     9121 taaagagttc ccggcgggga gctatttgtg gagtcaggac ggcgaaattc gttcttatta
     9181 tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca aaaacgagct
     9241 gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt
     9301 gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga aatacgcagc
     9361 ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac actcctttgc
     9421 tgtaggtctg ccgggttcac cggatctgaa agcagcccag gaagtggcaa accatctggg
     9481 cactgtgcat catgaaattc acttcactgt acaggaaggt ctggacgcca tccgcgacgt
     9541 gatttaccac atcgaaacct atgatgtgac cactattcgc gcttcaacac cgatgtattt
     9601 aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg ctgtccggtg aaggttctga
     9661 tgaagtgttc ggcggttatc tttacttcca caaagcaccg aatgccaaag aactgcatga
     9721 agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgtg ccaacaaagc
     9781 gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaagaaat tcctcgacgt
     9841 ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaagatgg aaaaacacat
     9901 cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc agaaagagca
     9961 gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg cggcgcagca
    10021 ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca caccgacctc
    10081 aaaagaagcg tatctgtacc gggagatctt tgaagaacta ttcccgcttc cgagcgccgc
    10141 tgagtgcgtg ccgggcggtc cttccgtcgc gtgttcttcc gctaaagcga tcgaatggga
    10201 tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg gttggtgttc accagtcggc
    10261 gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca gttattgagg
    10321 tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat cactgtttga
    10381 caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat tgttaaattc
    10441 cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg ggaaaaaaca caaagtaaac
    10501 aataattgac gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc
    10561 aattttcctt gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa
    10621 cgccgataag gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc
    10681 actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttttgcgg
    10741 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    10801 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    10861 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    10921 cttctttgag taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc
    10981 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa
    11041 tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    11101 gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa attcaaaaac
    11161 caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg
    11221 ttcgaatcct cgtaccccag ccaatttatt caagacgctt accttgtaag tgcacccagt
    11281 tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc
    11341 ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc
    11401 actcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg cgacgctgcc
    11461 gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct acaaccctaa
    11521 cgcatatttc agcgcctgac gtttcaacac gccagcacgc tccgccgcca tcaaaccaag
    11581 attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa acagatccat
    11641 accgctctgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct tgaggacagg
    11701 ataactggcc cacgcttcgc cgtagctgcg ggcattcacc agaacatcaa tcagggcatc
    11761 gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg tgtgcgcggc
    11821 atcacccacc agcgcaagcc ccggctgcac atactgcaac gcatggcggc gtgtcagcgg
    11881 aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat gcttcgcgat
    11941 ttccacctgt aactgcgcca tattcatatt ctgcaactgg cgaatgcgcg ccggagagtc
    12001 gtaccacacc agcgatgccc agttatcaaa cagcggcaga aacgcgcgcg gtccatccgg
    12061 ggtaaattgc tgccaggtgc tatcgccggg atcgttctcg cactggacgc taatcaacat
    12121 gcacgactgc gcatactgcc atgcatgaac gccaattccc gccatctgcc gcacctgcga
    12181 atttgcgccg tcggcaccaa tcaccagctt cgcgcgaatc acttcaccgc ctttcagctc
    12241 cagctcctga agatcattat ctcgatgtaa tgcaatcagc gaggttggca cacgtaacgt
    12301 tactttcgga tgcgcttcca gcgcctgcca caatgcctgt tgcaggacag tgttttctac
    12361 catatagcca agcaacggca actttaattc ggctgcgtca aataccacat gcgccgtttc
    12421 ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta ctgcatccca
    12481 gacccctaac cctttaagca atgataccga agccgcgctg atcgccgaga tccgcacgtc
    12541 cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg ttaccgtaaa
    12601 tccgtgctgt gccagcccca gcgccagtgc gccgccgacc attcctccgc cgacaatggc
    12661 aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc tttaagttta
    12721 caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc agcaaagcat
    12781 tacactatgc gccctgcatt cctggctact atttcgcaag agcaagtcga tgaccaaaaa
    12841 actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga agatggccga
    12901 tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag cggatgtgct
    12961 gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg
    13021 tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg gtggctgcgt
    13081 ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata ttatttttgg
    13141 gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg accgcagccc
    13201 ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg aaccgcgcgc
    13261 cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt gcacctactg
    13321 cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata ttctgtttga
    13381 gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa
    13441 cgcctggcgt ggtgaaaact acgacggcac caccggatcg tttgccgatc tgttgcgtct
    13501 ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc cgatcgaatt
    13561 caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct tcctgcatct
    13621 gccggtacag agcggttccg atcgcattct gaacctgatg ggacgtaccc atacggcgct
    13681 ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccagatattc agatcagttc
    13741 tgacttcatc gtcggcttcc ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct
    13801 gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac gtccgggtac
    13861 accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcagc gtctgtatat
    13921 tctgcaagaa cgtatcaatc agcaagcgat ggcgtggagc cgccggatgc tcggcaccac
    13981 ccagcgtatt ctggtagaag gtacatcgcg taagagcatc atggagcttt ccgggcgtac
    14041 ggaaaataac cgcgtggtca acttcgaagg cacgccggat atgatcggta aattcgtcga
    14101 tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc gtactgaaga
    14161 tgaaatgggt ctgcgcgtgg cagaaacacc agaatcggtg attgcccgta ctcgcaaaga
    14221 aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt tccggtgggc
    14281 cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc
    14341 gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac agataagagg
    14401 aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg
    14461 ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg tctcggcatc
    14521 gagatcaatc gccgcgataa ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct
    14581 gcggcagaca ttctgcgtag cctgtatgtc gatactgccc cgatgcgcgg tcagattcag
    14641 gatatcgaac cggaacagat ccaccttgcg attaaagaag cacgggtact ggagcaaagc
    14701 gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt
    14761 aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca tgacattacc
    14821 ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc ggcagttgat
    14881 gccctggagc gtcaggaaat tcgccgtatt ctgctgactc gtccggcagt agaagccggt
    14941 gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta tctgcgcccg
    15001 ctgtacgacg cgctgtttga aatgctgggt tttgagaaag tcgagaaact gattgagcgc
    15061 aacgttattg aagtcgcacc gctggcctat atgcgtggtc gtacgctgaa cgacgcattt
    15121 atcattctcg atgagagcca gaacactacc atcgaacaga tgaagatgtt cctgacccgt
    15181 atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga cctgccgcgt
    15241 aatactaaat caggcttacg tcatgccatc gaagtgctgg ccgatgtcga agagatcagc
    15301 tttaacttct tccacagcga agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac
    15361 gcctatgaag cctgggaaga agccgaacaa aaacgtaaag cggcgctggc ggcagaacgc
    15421 aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg
    15481 aagataactc cggattgccg gaagagagcc agtttcaaac atggctgaat gcggtgatcc
    15541 cgcagtttca ggaagagtcg gaagtgacga ttcgcgtggt cgataccgcc gaaagccaca
    15601 gtctcaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg
    15661 aagtgccgcc tggcatggag atgtcgctac tgggcgatct ggttatctgc cgtcagatgg
    15721 ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg catatggtgg
    15781 tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa gcagaagaaa
    15841 tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg tacattgccg
    15901 agaaagaata atttgccagc catttgactg gcagacgtcc ccgccgttgg tgattaacga
    15961 cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg acaattcaca
    16021 cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca gccaactttt
    16081 ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt ccgggcagaa
    16141 cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca
    16201 acgcgtccgc gacatcatga tcccccgctc ccagatgatt accctgaaac gcaaccagac
    16261 gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc cggtgattag
    16321 cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc cgtttatgcg
    16381 cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg tcgttcctga
    16441 aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc acatggcgat
    16501 cgttattgac gaattcggtg gggtttccgg tctggtaacc attgaagaca tcctggaact
    16561 gattgttggt gagattgaag acgaatatga cgaagaagat gatatcgact tccgtcagct
    16621 gagtcgtcat acctggaccg tgcgcgcact ggcttccatt gaagacttca acgaagcgtt
    16681 cggcacccac tttagcgatg aagaagtcga cactatcggt ggtctggtga tgcaggcatt
    16741 tgggcatctt ccggcacgtg gcgaaaccat cgacatcgac ggttaccagt tcaaagtggc
    16801 gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg actcacccca
    16861 gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt ttgcctcatt
    16921 aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct gcggaacgct
    16981 ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc
    17041 gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct ggggatttgg
    17101 cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg gcggaatgcc
    17161 tggcccggtt aacatcttcc tggtggtact gctggcggcg tatttgtcgc tgtataccgg
    17221 actgtttgct ggcgtgctgt cgcgcctgtg gccgaaaacc acctggctgc gcgtggcgat
    17281 tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac tgacaggctt
    17341 cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc tggcaccgct
    17401 aatgggcgtg gaagccatta acttcctgct tatgatggtt agcggcctgc tggcactggc
    17461 gttagtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg cccttccctt
    17521 cccgctgcgt tacatccggt ggtttacccc acaaccggag aaaaccattc aggtttcgat
    17581 ggttcagggc gatattccgc aatcgctgaa atgggacgaa ggccagcttc ttaatacgct
    17641 gaagatttac tacaacgcaa cggcaccgct gatgggcaaa tcatcgttga ttatctggcc
    17701 ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag cactggacgg
    17761 tgagttgcgt gataaaggta gctcgctggt gaccgggatt gttgacgcgc gtcttaataa
    17821 gcagaaccgt tacgatacct acaacaccat catcacactg ggtaaaggtg caccgtacag
    17881 ctacgaatca gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc
    17941 gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt cgtcgttcag
    18001 ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt attgagctta ctgcggctat
    18061 ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg ataccgacta
    18121 tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat ggcagcactt
    18181 ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa
    18241 cggcattacg gcggtgattg gcccgcaggg tgagattcag gcgatgatcc cgcagttcac
    18301 ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga ctcacaccat acgcacgtac
    18361 cggcaactgg ccgctgtggg tgctgacggc actgtttggt tttgctgctg tgttgatgag
    18421 tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg gaatacatcc catatttcgg
    18481 cacgctcacc ggatgcggca tcagtgcatt atccggataa caatattctc ctatccaact
    18541 ttggcacatc tattgctttg ttatacaagg caaagcttta accagcatca gtacaacccg
    18601 gttgcaccac agcaatccac cggtagcacc aaaagagtgc attttaagaa ttttgcttcg
    18661 gaatggtgcg gcgcacctcc caaatataaa caataaaaca gcaatatctt tacatttagc
    18721 caaactaaat gttacctaac aggcacaaca ctacacaata aagttgcaga tgataacaac
    18781 acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg agttggatat
    18841 gcaattacgt aaacctgcca cagcaatcct ggccctggcg ctttccgcag gactggcaca
    18901 ggcagatgac gccgccccgg cagcgggcag tacgctggac aaaatcgcca aaaacggcgt
    18961 gattgtcgtc ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa
    19021 agtggtgggt tattcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact
    19081 caacaaaccg gacttgcagg tgaaactgat tccgattacc tcacaaaacc gtattccgct
    19141 gctgcaaaac ggcacttttg attttgaatg tggttctacc accaacaacg tcgaacgcca
    19201 aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa
    19261 gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg
    19321 cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat
    19381 catcagcgcc aaagatcacg gtgactcttt ccgtaccctg gaaagcggtc gtgccgttgc
    19441 ctttatgatg gatgatgctc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa
    19501 ctgggatatc gtcggcaagc cgcagtctca ggaagcctat ggttgcatgt tgcgtaaaga
    19561 cgatccgcag ttcaaaaagc tgatggatga cactatcgct caggtgcaga cctccggtga
    19621 agcggaaaaa tggtttgata aatggttcaa aaatcccatt ccgccgaaaa acctgaacat
    19681 gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaagcact
    19741 gaactaatta caagaaccag gggcggaaat tctagccctc tcgattgtta cgtagcacgg
    19801 acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta
    19861 acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt
    19921 ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaac accacctatc
    19981 ttggttggat ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta
    20041 tcgctttcct cgtcggttca tttttcggta ttttacgtac cgttccaaac cgttttctct
    20101 ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct
    20161 ttacctggta tctggtgatc ccggagctgc tgccggagaa aatcggcatg tggtttaagg
    20221 ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta
    20281 ccgccgcccg tgtttgcgaa caggtacgcg ccgcgattca gtcgctgccg cgtgggcaga
    20341 agaatgccgc gctggctatg ggtctgacgc tgccgcaggc ttaccgctac gtactgctgc
    20401 ctaatgctta tcgcgttatc gtcccgccga tgacctcaga gatgatgaac ctggtgaaaa
    20461 actccgccat cgcctcgacc atcggtctgg tagatatggc agcgcaggcg agtaaattgc
    20521 tcgattactc ggctcacgcc tgggagtcgt ttaccgccat tacgctagcg tatgtgttta
    20581 ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg taaagttcgc ctgcctggca
    20641 acatgggggg caaataatgt acgagtttga ctggagttcc attgtccctt ccctgccata
    20701 tctgctcgac gggctggtga tcacgctgaa aatcaccgtc acggcagtcg tgattggtat
    20761 tttgtggggc accatgctgg cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt
    20821 tgccaaagcc tacgttaacg tattccgctc gattccttta gttatggttt tgctgtggtt
    20881 ttacctgatc gtgccgggtt ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat
    20941 ccgtttaatt tcggcgatgg tggcgttttc gatgtttgaa gcggcctact attcagagat
    21001 tatccgtgcc ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg
    21061 aatgactcac tggcagtcga tgaaactgat tattctgccg caggcgttcc gcgcgatggt
    21121 gccgctgctg ctcactcagg gcatcgtact gttccaggat acctcgctgg tgtatgtgtt
    21181 atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gcacgcaggt
    21241 tgagatgatc ctgtttgccg gatttgttta tttcgttatt agccttagcg cgtcgttgtt
    21301 ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg
    21361 tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg
    21421 gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc
    21481 gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaacga caagaaaacc
    21541 gatctggcaa agctgcgttc tcgcgtcggg atggtattcc aacatttcga gctgttccct
    21601 catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa
    21661 gcaccggctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc
    21721 aataaatttc cggcgcagct ttccggcggc cagcagcagc gtgtggcaat tgctcgcgca
    21781 ttgtgtatgg atcctattgc gatgctgttc gacgaaccca catcggcact agacccggag
    21841 atgatcaacg aagtactgga cgtgatggtg gaactggcga acgaggggat gaccatgatg
    21901 gtggtgaccc acgaaatggg ctttgcccgt aaagtggcga atcgggtgat ctttatggat
    21961 gagggtaaaa ttgttgaaga ctcgccgaaa gatgcgttct tcgatgatcc gaaatcggac
    22021 cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcgc atacccaagt
    22081 gcgcgccatc tcacgcttta tcgtctcgcc cctcgtatct gttacatcct ggcgttaccc
    22141 ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc
    22201 gacccaggtc atgatgacgc tatcgcaata gttctcgccc tcgcctcacc agagcttgat
    22261 gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt
    22321 ctgcgtatgc tgaccttgct taatcgcacc gatattccgg tggcaggcgg cgcggtaaaa
    22381 ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc
    22441 ccggcattac cggaaccgac gttcgcaccg caaaactgta cggcggtaga gctgatggcg
    22501 aaaacgctgc gtgaaagtgc ggaacctgtc acgattgtgt ctaccggacc gcaaactaac
    22561 gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt
    22621 atgggtggcg ctatgggact gggtaactgg acgcctgcgg ctgaatttaa tatttacgtt
    22681 gacccggaag cggcagaaat tgtcttccag tcagggatcc cggtggtgat ggccggtctg
    22741 gatgtaactc acaaagcaca aatccacgtt gaagacactg agcgtttccg cgcgattggc
    22801 aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac
    22861 gaaaaatggg gctttgtcgg cgcaccgcta catgacccat gcaccatcgc ctggctgtta
    22921 aaaccggagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat
    22981 acccagggta tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact
    23041 gtaatggtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt
    23101 tacgcttaac acacgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatctg
    23161 tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta
    23221 tcgatctcgg tactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa
    23281 ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc
    23341 atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag
    23401 cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta
    23461 acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc
    23521 tgcaacgtcc gattaaagat gtggtgatct ctgttccggc ttatttcagc gatgaacaac
    23581 gcaagcatac ccgtttagca gcggagttag ccggtttaaa tgcggtacgc ttaattaatg
    23641 aacccacagc agctgcgatg gcgtatggcc tgcataccca acaaaatacc cgttcgctgg
    23701 tgtttgatct cggtggcggc acgtttgacg tcacggtgct tgagtacgcc acgccggtga
    23761 ttgaagttca cgcctccgct ggcgacaact tcctcggtgg cgaagatttt acccatatgc
    23821 tggtcgatga ggttttaaaa cgcgcggatg tcgccaggac cacgcttaac gagagtgaac
    23881 tggcagcctt gtatgcctgt gtggaagcgg caaaatgtag caatcaatcg ccaatacaca
    23941 ttcgctggca gtaccaggat gaaacgcggg aatgcgaatt ttacgagaac gaactggaag
    24001 atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg
    24061 cgcgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc
    24121 cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgatc
    24181 cgagcaccat tgtcgcgctg ggcgcagcaa tccaggcagc ctgccgctta cgcagtgaag
    24241 atattgaaga agtgatcctc actgatattt gcccttactc gttgggcgtt gaggttaacc
    24301 gccagggcgt ttccggcatt ttctcgccga ttattgaacg aaacaccact gtgcccgtgt
    24361 cgcgtgtaga aacttattca accatgcacc cggaacagga ttctattacg gttaacgtct
    24421 atcagggaga aaaccacaaa gttaaaaaca acattctggt ggaatccttc gatgtgccgt
    24481 tgaagagaac cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggtt
    24541 tgcttgaagt tgacgtgctt ctggaagacg gcagcgttaa gtccagagtg attaaccaca
    24601 gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga
    24661 aaatttatcc gcgcgatatg ctcatcaacc gcacctttaa ggccaaactg gaagagttgt
    24721 gggcgcgggc gctggatgac gagcgagaag agattggccg ggtgatcacc gattttgatg
    24781 cggcgctgca gtcaaacgat atggcccgcg tcgatgaggt caggcggcag gcgagcgttt
    24841 atttagccat tgagatccca taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg
    24901 catcggcgca aaaaacacat cgtcaactaa acaacatcgc gattttgccg agagcataac
    24961 ctatcaccag caccgttaag ctgattttga taatgctggt aatgctctgt ggtttttcat
    25021 cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg
    25081 gctcgagttt tactttcgtc aacagcatcc gggtgataat ggcctctaat ggatgagcat
    25141 ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa
    25201 gtactacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc
    25261 taccgactct cgatttccac cacgcccatg ctttgcgttt gaagaatagc gactcaataa
    25321 acgattgacc taacggcgat agttttccgc tatgtaaaat ttccgcccat aacagacgcg
    25381 tccgggcgct ctcaccagga acatatttca acggtttacc ttgcgcatct accatgctcc
    25441 gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgttgt gcgctccact
    25501 gcaacaacaa attatcggct tgtgtagatg ggtaacttcc ccagtacgcg cagagatcgg
    25561 taagcaggct ttccccctcg ccgcagtaca aacgttgtgc acactggtaa taccgggcat
    25621 cttcgctttc tggttcttcc atttgccact gttcggctac aggaatgagt tccgccatac
    25681 ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa
    25741 tgaaccacgg accttccatc gccaaaaacg cagtgacata gtcaggatgg taacggcagc
    25801 aacgttccag cgcgaagtaa tactcaacag tctggttctg caacgcaacg ggatatacca
    25861 gaaggccaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt
    25921 cttactcatc tattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccagca
    25981 aagaagcaca atttaatgaa agatatcgcg cttctatagc gattgcacac agcggccagc
    26041 gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg
    26101 cggagggctg aaaacgatca ctttctgaat ccagtaatgc cctgaataca cgtagtattt
    26161 catgctcagt ggcatcatct ttttcttcat catctgcttc ctcgacagga tttaccgcca
    26221 gtcgcagggc ctcttcatag gcctggcgta atgctttaaa cccttgcgga tcggtttcgg
    26281 gatgacataa cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc
    26341 tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac
    26401 cgggcattca ggctggacag ttaatggctg ccagggaagc gtggcataga aacgatcgct
    26461 tcccgcagcc agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag
    26521 cgcctcgcgt agcgaagcgt tgtcttcgca ttgagaagat tgccagtaga gacgccctag
    26581 tgaataggcg gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gttcatagac
    26641 gcgcgattgc agccataaac tctcgtcttc cgtcagccac ttgttgagca caccgacccg
    26701 tgacagaaac cccattcgaa cataatccca agctttgatt cccccttccc cacaacacat
    26761 agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa
    26821 tgctttccac tcttcagggg aagagcgatg ccatgtcaga tataagccag ctaaatcatt
    26881 ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat
    26941 tccccaggat gatgagagat cgaattcttt tatcccggta tacatttttg gcgagtcatg
    27001 cgcgggtttg caaccctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata
    27061 ttgttcttcc ttattcattt gttaataatc ccttaagtgc attaactg
//
LOCUS       DAZGXS010000058        26864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGXS010000058 DAZGXS010000000
VERSION     DAZGXS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..213
                     /locus_tag="ACHKG5_002514"
     CDS             <1..213
                     /locus_tag="ACHKG5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0868598.1"
                     /translation="AELADDMGKGLPQHLFESLCIDHLQRHGASKKAITRAFDDDVEF
                     QERMAEHIRYMVETIAHHQVDIDSEV"
     gene            219..1004
                     /gene="bet"
                     /locus_tag="ACHKG5_002515"
     CDS             219..1004
                     /gene="bet"
                     /locus_tag="ACHKG5_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0868599.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            1001..1681
                     /locus_tag="ACHKG5_002516"
     CDS             1001..1681
                     /locus_tag="ACHKG5_002516"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0868600.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1678..1860
                     /locus_tag="ACHKG5_002517"
     CDS             1678..1860
                     /locus_tag="ACHKG5_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP0868601.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            1833..2024
                     /locus_tag="ACHKG5_002518"
     CDS             1833..2024
                     /locus_tag="ACHKG5_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0868602.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            2101..2316
                     /locus_tag="ACHKG5_002519"
     CDS             2101..2316
                     /locus_tag="ACHKG5_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0868603.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2415..2636
                     /locus_tag="ACHKG5_002520"
     CDS             2415..2636
                     /locus_tag="ACHKG5_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0868604.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2633..3580
                     /locus_tag="ACHKG5_002521"
     CDS             2633..3580
                     /locus_tag="ACHKG5_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0868605.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            3582..3760
                     /locus_tag="ACHKG5_002522"
                     /pseudo
     CDS             3582..3760
                     /locus_tag="ACHKG5_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4094..4450
                     /locus_tag="ACHKG5_002523"
     CDS             4094..4450
                     /locus_tag="ACHKG5_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868606.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            4447..4797
                     /locus_tag="ACHKG5_002524"
     CDS             4447..4797
                     /locus_tag="ACHKG5_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0868607.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPEKYDFDIWVFSPSRGVLDGLQWDGSMFTDDEYQFVIHDATHW
                     MRKVYPAAPQQDGE"
     gene            4985..5329
                     /locus_tag="ACHKG5_002525"
     CDS             4985..5329
                     /locus_tag="ACHKG5_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868608.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            5411..5602
                     /locus_tag="ACHKG5_002526"
     CDS             5411..5602
                     /locus_tag="ACHKG5_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0868609.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(5583..6761)
                     /locus_tag="ACHKG5_002527"
     CDS             complement(5583..6761)
                     /locus_tag="ACHKG5_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0868610.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            6943..8367
                     /gene="sbcB"
                     /locus_tag="ACHKG5_002528"
     CDS             6943..8367
                     /gene="sbcB"
                     /locus_tag="ACHKG5_002528"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0868611.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(8410..8637)
                     /gene="tsuB"
                     /locus_tag="ACHKG5_002529"
     CDS             complement(8410..8637)
                     /gene="tsuB"
                     /locus_tag="ACHKG5_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP0868612.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(8651..9709)
                     /gene="tsuA"
                     /locus_tag="ACHKG5_002530"
     CDS             complement(8651..9709)
                     /gene="tsuA"
                     /locus_tag="ACHKG5_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP0868613.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(9888..11246)
                     /gene="plaP"
                     /locus_tag="ACHKG5_002531"
     CDS             complement(9888..11246)
                     /gene="plaP"
                     /locus_tag="ACHKG5_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0868614.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11236..11298)
                     /gene="yoeI"
                     /locus_tag="ACHKG5_002532"
     CDS             complement(11236..11298)
                     /gene="yoeI"
                     /locus_tag="ACHKG5_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0868615.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11513..12442)
                     /gene="yeeY"
                     /locus_tag="ACHKG5_002533"
     CDS             complement(11513..12442)
                     /gene="yeeY"
                     /locus_tag="ACHKG5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0868616.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12488..13312)
                     /gene="yeeZ"
                     /locus_tag="ACHKG5_002534"
     CDS             complement(12488..13312)
                     /gene="yeeZ"
                     /locus_tag="ACHKG5_002534"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0868617.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            13738..13788
                     /gene="hisL"
                     /locus_tag="ACHKG5_002535"
     CDS             13738..13788
                     /gene="hisL"
                     /locus_tag="ACHKG5_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0868618.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    13764..13887
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13934..14833
                     /gene="hisG"
                     /locus_tag="ACHKG5_002536"
     CDS             13934..14833
                     /gene="hisG"
                     /locus_tag="ACHKG5_002536"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0868619.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            14839..16143
                     /gene="hisD"
                     /locus_tag="ACHKG5_002537"
     CDS             14839..16143
                     /gene="hisD"
                     /locus_tag="ACHKG5_002537"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0868620.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            16140..17210
                     /gene="hisC"
                     /locus_tag="ACHKG5_002538"
     CDS             16140..17210
                     /gene="hisC"
                     /locus_tag="ACHKG5_002538"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0868621.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            17210..18277
                     /gene="hisB"
                     /locus_tag="ACHKG5_002539"
     CDS             17210..18277
                     /gene="hisB"
                     /locus_tag="ACHKG5_002539"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0868622.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            18277..18867
                     /gene="hisH"
                     /locus_tag="ACHKG5_002540"
     CDS             18277..18867
                     /gene="hisH"
                     /locus_tag="ACHKG5_002540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0868623.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            18867..19604
                     /gene="hisA"
                     /locus_tag="ACHKG5_002541"
     CDS             18867..19604
                     /gene="hisA"
                     /locus_tag="ACHKG5_002541"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0868624.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            19586..20362
                     /gene="hisF"
                     /locus_tag="ACHKG5_002542"
     CDS             19586..20362
                     /gene="hisF"
                     /locus_tag="ACHKG5_002542"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0868625.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            20356..20967
                     /gene="hisIE"
                     /locus_tag="ACHKG5_002543"
     CDS             20356..20967
                     /gene="hisIE"
                     /locus_tag="ACHKG5_002543"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0868626.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(21064..22041)
                     /gene="wzzB"
                     /locus_tag="ACHKG5_002544"
     CDS             complement(21064..22041)
                     /gene="wzzB"
                     /locus_tag="ACHKG5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0868627.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(22187..23353)
                     /gene="ugd"
                     /locus_tag="ACHKG5_002545"
     CDS             complement(22187..23353)
                     /gene="ugd"
                     /locus_tag="ACHKG5_002545"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0868628.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(23602..25008)
                     /gene="gndA"
                     /locus_tag="ACHKG5_002546"
     CDS             complement(23602..25008)
                     /gene="gndA"
                     /locus_tag="ACHKG5_002546"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0868629.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(25209..25874)
                     /gene="perB"
                     /locus_tag="ACHKG5_002547"
     CDS             complement(25209..25874)
                     /gene="perB"
                     /locus_tag="ACHKG5_002547"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFP0868630.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            complement(25918..26849)
                     /locus_tag="ACHKG5_002548"
                     /pseudo
     CDS             complement(25918..26849)
                     /locus_tag="ACHKG5_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         7156 a         6614 c         6568 g         6526 t
ORIGIN      
        1 gcagaactgg cagacgacat gggaaaaggt ctgccccagc acctgtttga atcactctgc
       61 atcgatcatt tgcaacgcca cggggccagc aaaaaagcca ttacccgtgc gtttgatgac
      121 gatgttgagt ttcaggagcg catggcagaa cacatccggt acatggttga aaccattgct
      181 caccaccagg ttgatattga ttcagaggta taaagcggat gagtacagca ctcgcaacgc
      241 tggcagggaa gctggctgaa cgtgtcggca tggattctgt cgacccacag gaactgatca
      301 ccactcttcg ccagacggca tttaaaggtg atgccagcga tgcgcagttc atcgcattgt
      361 tgatcgtcgc caaccagtac ggccttaatc cgtggacgaa agaaatttac gccttccctg
      421 ataagcagaa cggcatcgtt ccggtggtgg gcgttgatgg ctggtcccgc atcatcaatg
      481 aaaaccagca gtttgatggc atggactttg agcaggacaa tgaatcctgt acatgccgga
      541 tttaccgcaa agaccgtaat catccgatct gcgttaccga gtggatggat gaatgccgcc
      601 gcgaaccatt caaaacccgc gaaggcagag aaatcaccgg cccgtggcag tcgcatccca
      661 aacggatgtt acggcataaa gccatgattc agtgtgcccg tctggccttc ggatttgctg
      721 gtatctatga caaggatgaa gccgagcgca ttgtcgaaaa tactgcatac actgcagaac
      781 gccagccgga acgcgacatc actccggtta acgatgaaac catgcaggag attaacactc
      841 tgctgatcgc cctggataaa acatgggatg acgacttatt gccgctctgt tcccagatat
      901 ttcgccgcga cattcgcgca tcgtcagaac tgacacaggc cgaagcagtg aaagttcttg
      961 gattcctgaa acagaaagcc tctgagcaga aggtggctgc atgacaccgg acattatcct
     1021 gcagcgtacc ggaatcgacg tgagagctgt cgaacagggg gatgatgcat ggcacaaatt
     1081 acggctcggc gtcatcaccg cttcagaagt tcacaacgtg atagcaaagc cccgctcagg
     1141 aaagaagtgg cctgacatga aaatgtccta cttccacacc ctgcttgccg aggtttgcac
     1201 cggtgtggct ccggaagtta acgctaaagc actggcctgg ggaaaacagt acgagaacga
     1261 cgccagaacc ctgtttgagt tcacttccgg cgtgaatgtt attgaatccc cgatcatcta
     1321 tcgcgacgaa agtatgcgta ccgcctgctc tcccgatggt ttatgcagtg acggcaatgg
     1381 ccttgagctg aaatgcccgt ttacctcccg ggatttcatg aagttccggc tcggtggttt
     1441 cgaggccata aagtcggctt acatggccca ggtgcagtac agcatgtggg tgacacgaaa
     1501 agatgcctgg tactttgcca actatgaccc acgtatgaag cgtgaaggac tgcattatgt
     1561 cgtggttgag cgggatgaaa agtacatggc gagttttgac gagatggtgc cggagttcat
     1621 cgaaaaaatg gacgaggcac tggctgaaat tggttttgta tttggggagc aatggcgatg
     1681 aagcatcctc acgataatat ccgggtaggt gcgatcactt tcgtctactc cgttacaaag
     1741 cgaggctggg tatttcccgg cctttctgtt atcagaaatc ccctgaaagc acagcggctg
     1801 gctgaggaga taaataataa acggggagct gtatgcacaa agcatctccc gttgagttaa
     1861 gaacgagtat cgagatggca catagcctcg ctcaaattgg agtcaggttt gtgccaatac
     1921 cagtagaaac agacgaagaa tttcatacgt tagccgcatc cctttcacaa aagctggaaa
     1981 tgatggcggc gaaagcagaa gcaaacgaga gagacccggc atgacaacaa cagaatgcat
     2041 ttttctggca gcaggcttca tattctgtgt gcttatgctt gccgacatgg gacttgttca
     2101 atgacacctc agcaggaaaa cgcccttcgc agcattgccc gtcaggctaa ttctgaaatc
     2161 aaaaaagcca gacagcagtt tccggataaa aacgtcgatg acatttgccg tagcgtactg
     2221 aagaagcacc gcgaaacggt aacgctaatg ggattcacac cgactcattt aagtctggcg
     2281 atcggcatgt taaacggcgt ctttaaggag cgatgaacat gaaaagcaaa atcatcaggg
     2341 agctacaggc tcctttttta ttgttcgcat tcaccctcaa gcgtattaac caacaattca
     2401 gggattaatg gaagatggca gacatcattg attcagcatc agaaattgaa gaattacagc
     2461 gcaatacagc aataaaaatg cgtcgcctga actaccagac tgtatccgca actcattgtt
     2521 gtgagtgtgg cgatccgata gatgaacgaa gacgcctggc agttcagggt tgtcggactt
     2581 gtgcaagttg ccaggaggat ctggagctta tcagtaaaca gagaggttcg aagtgagcga
     2641 aattaattat caggcactgc gtgaaaaggc agagaaagca actaaaggaa gctacatcgt
     2701 agggcataca tctgttaacc agcacggcaa tttaacagga gtttttgttt gccaaaaatg
     2761 gaaaggagaa cccggtggtg tgattgcgga atgtcatgtt aactgcctgg ttgaaacaga
     2821 tgttcaggct tatgcaaacg ctgaatttat tgctgccttt aatccaaatg ttgcgctggc
     2881 gcttctggat gaacgggaaa gaaaccagca atacatcaaa cgccgcgacc aggagaacga
     2941 ggagattgcg cttacggttg ggaagctgcg tgttgagctt gaagcagcaa aatcaaaact
     3001 caacgagcag cgtgaatatt acgagggagt aatcgcggat ggaagtaagc gcatagcaga
     3061 actggaaaaa caatgcgccg aatgggagcg aaaagcatta agcaactttg aagagtgtgc
     3121 tgcgatggct gaacgtatcg aagagatgca gacaaaatct gcaccagatt cgtttggcat
     3181 catcggtgaa aatattcgaa cacaggacaa tcgaataacg tcagatccca tgttttgtgt
     3241 gtatcaaaag cgcgaaatcg ctgttgatgc tgattatgac catgaccgga ttgtctgggt
     3301 tgacgaagat ggcaatgaag ccaataaacg ccatagtcgt cgtctcgagc tacttcatga
     3361 aaactttcga gagccaccag aaaaatggcg gcgcgttgct gtgaaagata ttgatgaatt
     3421 cgttacctgc tgtttcaccg aacagggttg taaagactac ctggcagtca atggtcacaa
     3481 tcttcgcttg ccatttatat atgtaaaaag cggtttcagg aacgctgaat atatcggcat
     3541 aagaaactgg cttgctggca ttcgcatcaa aggagagtga tatggcgtta acacaccgcg
     3601 aactctgtca gattgcgtac aagttcctta agcgcaacgg tttcaaggtt tgttttcatg
     3661 accgctttat agctgtaacc agtaccggag aacagccaga tgctatggga ttcagaaatt
     3721 cagcatcatg cctgatagag gcgaaatgtt ctcgtgctga cttgttggca gatagaaaaa
     3781 agcgttttcg taaaaatccg tctcttggaa tgggcgactg gcgattcttt attagtgagc
     3841 cgggaattat ttcaattgag gatttaccac ctggctgggg attacttcac gttgttaacg
     3901 gaagagtacg gaaagtacat gggtggccca agggtaattg ctgttggggt aatcctgacg
     3961 ataagccatt tactggaaat aagcaggttg aatgcgatta catgttgtct gcattaaggc
     4021 gcatggagtt gagagggcac cttaatgaaa tatatgacgg tgtaattgtt aataagaaag
     4081 aaggaaacgc ggcatgacca ctattaccga caaagaacta attaaagaaa tcaaagagcg
     4141 cataggcagc ttggacgttc gagacaatat tgagcgccgt gcttatgaaa ttgcactggc
     4201 atcgctggaa gcagaaccga tagcgtggga atgcggtgaa aacataatcc tgtttaaccc
     4261 tgacacagtt gaagcatacg caaaacgtgc ggaaatatca cctaaaccac tattctccgc
     4321 gccgccagcg ctggtagtgc ctgataagtt gccgcgtgaa tacagaaacg gttggcctct
     4381 tgcgtatagt gattatgctg aaggctggaa cgactgccgc gaagccatgc ttcagggaga
     4441 taaatcatga ttaatcgtat caagctggag cacatcctcg aatatgccag gcagcagagg
     4501 catattggtc agcattgtaa aattccacca ggagatatgg ttgaaatcat ggagattgcc
     4561 atgcgcaagg ctggcaactc tccggtaact ccggatggtt ggataagctg tagtgagcga
     4621 atgccggaga aatacgattt cgatatctgg gtattctctc catccagagg tgttcttgac
     4681 gggctacagt gggatggctc gatgtttact gatgatgaat atcagttcgt tattcatgac
     4741 gccacgcatt ggatgagaaa agtatatcca gcagcaccgc agcaggatgg tgaatgatgc
     4801 cgccagttaa agttgtgatt atcactttgg tgatgatagt gattgcgaga atcatgtctg
     4861 gtgaaattgt gtggatatgg taatggctaa ggcagcagca gagcgcaact aacaatcctc
     4921 gcactcgcgg ggatttcttt tatctgaact cgctacggcg agttttgttt tatggagaca
     4981 agaaatgtca gatttggcta tgaaggtttt gaaatggcaa tcaactggcg atgtcggcat
     5041 cagtagcgca actcttgcct caatcgcatg tggcctgaaa aagaatatct atggtcatgg
     5101 cttcggtgct ccacatgacg cagccgattt cagacgatgc gttgcacttg ttgagcagat
     5161 tccagaaatc agagattcat tcgacaaggt tgcaaagcgc gttccggcat tcaaaggaat
     5221 cctcaacgaa tgggattctc tcgttgctct gttgaagtct gaaatgaaga tacacggaaa
     5281 caaagcacca gagacttaca gaagaattag cgagttacgc aaggactaac cacagcctca
     5341 cactcgatga ggcctgtaca tatctgatag agccgctata tggcggtttc tttttgcctg
     5401 gagaattaag atgaccgata ccagcctgat tcctgagaaa gaagtgatga acaagctcgg
     5461 tgtttcatca cgtcagacaa tctggaacta taccaaacgg cacggatttc cgaagccagt
     5521 cagaacccac cccaaatcat accttcgtga agctgttgag gggtggattc ttaacggtgg
     5581 cgttaatcag aaatgctcct gatgtgccag aacaatttat cagcgtgaag ctcatacgct
     5641 ttgcgctgag cttctatcca gtcgtgttta ttgtatacgg acattatccc gccaagttca
     5701 tgaccgagca ttcgctcagt aacatgaggc tcgacattca tctcggacag acgtgtaaca
     5761 agcgttcttc tgaaatcatg tgttcgccag taacctagat ccatccctcc ccttattcta
     5821 tttacgaatc ggttggctgc agcgatgctg atcggtttgt ttatgtcttc tccagggaaa
     5881 agaacatcat tgtacgttgt catggccttt tcgagaaaag gcttaatttg ttcgaagatt
     5941 ggacgacgga taacattacc catcttgcta tgctctttcg gtacggtcca tacgttgtcc
     6001 agcaggtcaa attctgtctt tgttgccagc ctaagctctg agagcctcgc cccccacagc
     6061 ataagcatct gatgaaggag cttatttgac gtagacgcac ggcttctttc aatagcaagc
     6121 caaatcttag ccagttcgtg atacgacagt acccgatccc ctacctcagc gcgggagccg
     6181 aagtcccttg gctggatgct cataattgcg caactatcta tcaactgacg ccgcatgcac
     6241 caactaattg ctgatcttag ttgacttagc acctgccttg ctcggcgtgg attatctttt
     6301 tcttcttcgg taagcaggtc aacccattgc ttaaccgtga tagaagatgc cggacgatta
     6361 ggaaaggcgt catgcatgcg cttcataacc gttgatcggt aaagtgcctg ggtcttttct
     6421 ctgagagttg tagagacgta gttgtcgaac cagtagtcga gacactgggc gaccgtcatg
     6481 gagttctcca ccttctcttc aaaataagtg cgtggatccg ttcctgagaa atagagcttt
     6541 cgcaagtcag cagtgatctg tctggcatcc ttcaaagaca gggatgggta tcgaccaagc
     6601 ccaagtcgat taggcttgcc atgccagcga tagcggtact ggaactggat gacccccttc
     6661 ggtgaaattc gtacgctgag gccatcggca tcagccactt cttgtgggcc cgaatatggt
     6721 ttaccataaa tagtacgcag ttttgtgtcg cttatagcca taaataatat ttggtacgct
     6781 caaaaataat ggtttggtac acatctggta cacaatatca catggacaac agcgcacagt
     6841 aaacaactat atttgacgtt cgtagacata ctcaggtgaa aaaatggttg tttttcgagt
     6901 tataacagac agttaattaa ctacagtgga caatgctaga caatgacaga cacagataaa
     6961 caacctacct tcctctttca cgattacgaa acctttggca cgcaccccgc gttagatcgc
     7021 cctgcacagt tcgcagccat tcgcaccgat gacgaattca atgtcatcgg cgaacccgaa
     7081 gtcttttact gcaagcccgc ggatgactat ttaccccagc ctggagcagt attaattacc
     7141 ggtattaccc cgcaggaagc tcgggcgaaa ggagaaaacg aagccgcatt tgccgcccgt
     7201 attcactcgc tttttaccgt accgaagacc tgtattctgg gctacaacaa tgtgcgtttc
     7261 gacgacgaag tcacacgcaa cgttttttat cgtaatttct acgatcctta cgcctggagc
     7321 tggcagcatg ataactcgcg ctgggattta ctggatgtta tgcgtgcctg ctatgccctg
     7381 cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt tcgccttgag
     7441 catttaacca aagcgaatgg tattgaacat agcaacgccc acgatgcgat ggctgatgtg
     7501 tacgccacta ttgcgatggc gaaactggta aaaacgcgtc aaccacgcct gtttgattat
     7561 ctctttaccc atcgtaataa acacaaactg atggcgttga ttgatgttcc gcagatgaaa
     7621 cccctggttc acgtttccgg aatgtttggg gcatggcgcg gcaataccag ctgggtggca
     7681 ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta tggtggattt ggcaggagat
     7741 atttcgccat tactggaact ggatagcgac acattgcgcg agcgtttata taccgcaaaa
     7801 accgatcttg gcgataacgc cgccgttccg gttaaactgg tacatatcaa taaatgtccg
     7861 gtgctggccc aggcgaatac gctacgcccg gaagatgccg accgactggg aattaatcgt
     7921 cagcattgcc tcgataacct gaaaattctg cgtgaaaatc cgcaagtgcg tgaaaaagtg
     7981 gtggcgatat tcgcggaagc cgaaccgttt acgccttcag ataacgtgga tgcacagctt
     8041 tataatggct ttttcagtga cgccgatcgt gcagcaatga aaattgtgct tgaaaccgag
     8101 ccgcgtaatt taccggcact ggatatcact tttgttgata aacggattga aaagctgttg
     8161 ttcaattatc gggcacgcaa cttcccgggg acgctggatt atgccgagca gcaacgctgg
     8221 ctggagcacc gccgccaggt cttcacgcca gagtttttac aaggctatgc agaagagatt
     8281 cagatgctgg cgcagcagta tgccgacgat aaagaaaaag tggcgctgtt aaaagcgctt
     8341 tggcagtacg cggaagagat tgtctaatgt gaagcctccg ccgctggtac ggcggaggac
     8401 aatgttgagt caggcttttt gaacggtgat gctccacgct gcatcgccaa tctgctgata
     8461 atcggtgatg gcatgccctt cttctgccgc ccactgtggg attgcttcag ttgcctgggt
     8521 gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg ctgcttttgc
     8581 ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa tcgccatctt
     8641 tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc ggtcgaacat
     8701 acacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca acccagccct
     8761 gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca cctgcaatac
     8821 tggcaccgaa gcccatcagt acaccaccta gcccgctacg tactgttgtt tgtgcatcag
     8881 ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgccccc acgaagatcc
     8941 ctaacaccag gaaaacgccc cagttaatgt atttcacgtc acccgcgacc agaaattgca
     9001 gaatattggc cgttggggaa gtgatgccta acccaaacat acgtccggtt gcttcactca
     9061 ggggccaggc taaaagcgca atcaaaccga tgagtacagc tgtgacaaag ggatgccagc
     9121 gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc gcgactttga
     9181 gttttggctt cttcaactct ttcatcacca cccagagcgt tatcaccagc aaaacggcaa
     9241 ccaacggcca cacagacaaa ttaaaagtct cagcaataga gttatgttca gtagtgtagt
     9301 gctgcaaggt ttgatttaaa ccactggcgt gtggagaacg catcaccgca ctcatcacca
     9361 tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga taccaggtcc
     9421 cggtggcaca tccacccgcc agaacaattc ccagcccgaa gagataccca cctataacag
     9481 taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga attaacgcaa
     9541 aaacccccac gctttgtacc gaaattgcaa tcagcaaggc gtaaaacatg cgattatttt
     9601 tcgcgatata catatcgcga aaaccaccag tcaggcaaaa acgcccacgc tgcatgacaa
     9661 atcccagcag agcaccacaa attagcccgc ttaatatcat tgaaaacata aattaattaa
     9721 ccagatgaat gttaatgggg aaattattca tgactggtgg aatgcagacc aataaccaaa
     9781 ttctctaaat tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaac
     9841 cggagccaaa tgttcattcg actccggttt attattagaa atgatgatta tgctacgtct
     9901 tcgtactgcg gaaccgggtt acggaagctc ttggtgacgc aagccaggta aatcagaccg
     9961 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
    10021 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
    10081 ttattacgct tctcacggat ccagaactgc gagatgaccg acaggttaac gaaggtgaac
    10141 gccaccaacg caccaaagtt gatcagcgcc gtagccatta ccaggtcgaa gttgattgcc
    10201 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
    10261 acataaccga agaaactttt cgggaacacg ccgtcacgac ccattacgta catcagacgc
    10321 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
    10381 gcaccgacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
    10441 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
    10501 aagatcatgc cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
    10561 tctttggttt cttccgacag attgctgatg ccgtcaaagc cggtaaagga gaagcacagg
    10621 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa tggacgagtg
    10681 ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
    10741 accgcgatca gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc
    10801 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
    10861 gggatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
    10921 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
    10981 gatttctggg cgtaagtata tgcagagcca gcagaaggat agcggcgaac cagcttcccg
    11041 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
    11101 ccatccgtga ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg ctgcatatag
    11161 gcaagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
    11221 gtgtttggag taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta
    11281 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
    11341 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
    11401 taaccgacgc tgatgaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct
    11461 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg
    11521 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
    11581 catcccgcca ccagccgctg gcaagtcctg cggtctgttc attccagcca atccagacgg
    11641 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
    11701 gttgcgccag ataacggggt aaatagccgc agccgaggcc gctaatttgc agctccagct
    11761 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
    11821 aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac
    11881 ggtttaatgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
    11941 ccagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
    12001 caacaataat atccgctcgc ccctgagtca gggcatccca tgaaccaccg agtacgccat
    12061 tgataaactt caggcgcgtc acgttatgat gttgataaaa agcttcaatg agtggcgcaa
    12121 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
    12181 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccccttct
    12241 ctaataacat ttttccggta cgggtgaatt tagcgcggtg accgctacga tcaagcaatt
    12301 gaatattaag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
    12361 agagtttagc cgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca
    12421 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
    12481 tgatcgttta ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt
    12541 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
    12601 attgcggagg ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
    12661 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
    12721 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcaggcg
    12781 cggtttttcc ggcaaagaag cgtccgggat gacgccccgg tccgaccagg cccgcaagac
    12841 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
    12901 ctcgtccact gttggtcacc ggattacgcg gggtggtttc tttcaccgtg ccttgcgcgt
    12961 cgccatagac agacgttgaa ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc
    13021 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacggg
    13081 ccggaagcgt aatgaccagc gcatcggcat ccatcagggc atccagatcg tcagaatcgc
    13141 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
    13201 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
    13261 tcgccagcgg catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc
    13321 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
    13381 atgaatgaaa tcgaaactgt tagcaaatat accattgcct gatgcgctcc gcttattagt
    13441 cctacaaaac atcaatatat tgaatttagg caacatttgt agaccagata aggcattcac
    13501 gccgcatccg gcaggaacaa agcccacttt agttacagaa cacaatgttc ttgagggttt
    13561 taatgacata ttttcacgat tactttttta acctagtcac tcttagcccc ctcctttccc
    13621 cgctcattca ttaaacaaat ccatcgccat aaaatatata aaaaagccct tgctttctaa
    13681 cgtgaaagtg gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg
    13741 acacgcgttc aatttaaaca gcaccatcat caccatcatc ctgactagtc tttcaggcga
    13801 tgtgtgctgg aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg
    13861 aagatcacct tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa
    13921 gaggaataag aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg
    13981 tttaagtgat gactctcgcg aattgctggc gcgctgtggc attaaaatca atcttcacac
    14041 ccagcgcctg atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga
    14101 cgacattccc ggtctggtaa tggacggcgt ggtagatctt gggattatcg gcgaaaacgt
    14161 gctggaagaa gagctgctta accgccgcgc ccagggtgaa gatccacgct actttaccct
    14221 gcgtcgtctg gatttcggcg gctgccgtct ttcgctggca acgccggttg atgatgcctg
    14281 ggacggcccg ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa
    14341 gcgttatctc gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga
    14401 agtcgccccg cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac
    14461 gctggaagca aacggcctgc gcgaagtcga agttatctat cgctcgaaag cctgcctgat
    14521 acaacgcgat ggcgaaatgg aagaatccaa acagcaactg atcgacaagc tgctgacccg
    14581 tattcagggt gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccca
    14641 acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct
    14701 ggcgggtgat caacagcgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga
    14761 aacgatggaa aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa
    14821 gatgatggag tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgca
    14881 aagcaacaac gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc
    14941 actgttaacg atattctcga tagcgtgaaa gcacgcggtg atgacgccct gcgggaatat
    15001 agcgcgaagt ttgataaaac cacggttacc gcactgaagg tgtctgctga ggaaattgcc
    15061 gccgccagcg aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat
    15121 attgaaacct tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc
    15181 gtgcgttgcc agcaagtcac gcgcccggta gcttcagttg ggttgtatat tcctggcggc
    15241 tccgccccgc tcttctcaac ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt
    15301 aaaaaagtgg tgttgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag
    15361 ctgtgcggtg tgcaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg
    15421 tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc
    15481 accgaggcaa aacgtcaggt gagccagcgt ctggacggtg cggcgatcga tatgcccgca
    15541 ggcccgtcgg aagtgttggt cattgccgac agcggcgcaa cgccggattt cgtggcttct
    15601 gatttgcttt ctcaggctga acacggcccg gactcacagg tgattttact gacgcctgac
    15661 gctgatatgg cgcatcaagt tgccgaagcc gtcgaacgcc agttagcaga actgccgcgt
    15721 gccgaaaccg cccgccaggc actgaacgcc agccgcctga tcgtgactaa agatttagcg
    15781 cagtgcgtgg agatctccaa ccagtacggc ccggagcacc tgatcattca gacccgcaac
    15841 gcccgcgaac tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca
    15901 ccggaatcgg caggtgatta cgcctccgga accaaccatg ttctaccgac ttacggctac
    15961 accgccacct gttccagcct cggactggcg gatttccaga agcggatgac cgtgcaggaa
    16021 ctgtcgaaag taggtttctc cgcgctggct tcgaccattg aaacactggc cgccgccgag
    16081 cgcctgaccg cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca
    16141 tgagcaccgt gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc
    16201 agtcggcgcg tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatatccca
    16261 cagccgtgga gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgccagccga
    16321 aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca
    16381 gccgtggcgc ggacgaaggt attgaactac tgattcgcgc tttttgcgaa ccgggtaaag
    16441 acgccatcct ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg
    16501 gcgtcgagtg ccgcacagtg ccgacgctgg aaaactggca actggactta cagggcattt
    16561 ccgacaagct ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccaaccgggc
    16621 aactgatcaa tccgcaggat tttcgcaccc tgctggagtt aacgcgcggt aaggcgattg
    16681 tggttgccga tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctgg
    16741 cggaatatcc gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcgggcc
    16801 ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgattg
    16861 ccccctaccc gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg
    16921 gaattatcgc catgcgcgaa cgggtggcgc aaattattgc agaacgcgaa tacctgattg
    16981 ccgcactgaa agagatcccc tgcgtggagc aggtttttga ctctgaaacc aactacattc
    17041 tggcgcgctt taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct
    17101 tacgtgatca gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc
    17161 gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa
    17221 gtatcttttt atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt
    17281 ggatcgtttt gacaaactcg cctttgaacc gggcgtgatc ccgcaactgc tgaagctgca
    17341 aaaagcgggc tacaaactgg tgatgatcac taatcaggat ggtctgggaa cacaaagttt
    17401 cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg cagatattca cctcgcaagg
    17461 cgtacagttt gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg
    17521 taaaccgaaa gtaaaactgg tggagcgtta tctcgctgag caagcgatgg atcgcgccaa
    17581 cagttatgtg attggcgatc gcgcgaccga tattcaactc gctgaaaaca tgggcattaa
    17641 tggtttacgc tacgaccgcg aaaccctgaa ctggccaatg attggcgagc aactcaccag
    17701 acgtgaccgt tacgctcacg tagtgcgtaa taccaaagag acgcagattg acgttcaggt
    17761 gtggctggat cgtgaaggtg gcagcaagat taataccggc gttggcttct ttgatcacat
    17821 gctggatcag atcgccaccc acggcggttt ccgtatggaa atcaacgtca aaggcgacct
    17881 ctatatcgac gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa
    17941 aattgccctc ggcgataaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga
    18001 atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagccga
    18061 gtttacctac caacgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc
    18121 gctctcttac accatgggcg tgacgctcca cctgaaaacc aaaggtaaaa acgatcatca
    18181 ccgtgtagag agcctgttca aagcctttgg tcggaccctg cgccaggcca tccgcgtgga
    18241 aggcgatacc ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatact
    18301 ggctgcgcca acctgaactc ggtgaagtct gccattgcgc gtcacggtta tgaacccaaa
    18361 gtcagccgtg acccggacgt ggtgttgctg gccgataaac tatttttacc cggcgttggc
    18421 actgcacaag cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc
    18481 tgtacccaac cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagtgaa
    18541 gagagcaacg gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa aatgaccgac
    18601 tttggtctgc cgctgccgca tatgggctgg aatcgcgttt acccgcaggc aggcaaccgc
    18661 ctgtttcagg ggattgaaga cggcgcgtac ttttactttg ttcacagcta cgcaatgcca
    18721 gtcaatccgt ggaccatcgc ccagtgtaat tacggcgaac cattcaccgc ggcggtacaa
    18781 aaagataact tctacggcgt gcagttccat ccggagcgtt ctggtgccgc tggcgctaag
    18841 ttgctgaaaa acttcctgga gatgtgatga ttattccggc attagattta atcgacggca
    18901 ctgtggtgcg tctccatcag ggcgattacg gcaaacaacg cgattacggt aacgacccgc
    18961 tgccgcgctt acaggattac gccgcacaag gtgccgaagt gctgcatttg gtggatctga
    19021 ccggggcaaa agatccggca aaacgtcaaa tcccgctgat taaaaccctg gtcgctggcg
    19081 ttaacgttcc ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac
    19141 tggaagctgg ggttgcgcgc gtggtggttg gctccactgc ggtgaaatca ccagaaatgg
    19201 tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta
    19261 ttgacgagca aggcaacaag caggtggcgg tcagcggctg gcaagagaat tcgggcgtgt
    19321 cactggaaca actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg
    19381 atatctcgcg cgacggcacg ctggcaggct ctaacgtctc tttatatgaa gaagtgtgcg
    19441 ccagatatcc gcaggtggca tttcagtcat ccggcggcat tggcgacatt aacgatgttg
    19501 ctgccctgcg tggcactggc gtgcgcggcg taatagttgg tcgtgcatta ctggaaggta
    19561 aattcaccgt gaaggaggcc atctcatgct ggcaaaacgc ataattccat gtctcgacgt
    19621 tcgtgatggt caggtggtaa aaggcgtaca gtttcgcaac catgaaatca ttggcgatat
    19681 cgtgccgctg gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat
    19741 caccgcttcc agcgatggcc gtgtggtaga taaaagttgg gtatctcgcg tggcggaagt
    19801 gatcgacatt ccgttctgcg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat
    19861 tctttccttt ggcgcggata aaatttccat caactcccct gcgctggcgg acccgacgtt
    19921 aattactcgc ctggcggatc gatttggcgt gcagtgtatt gtggtgggta ttgatacctg
    19981 gtacgacgct gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac
    20041 ccgcgtcact cagtgggaaa cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg
    20101 agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgaaca
    20161 actgaaaaaa gtgcgtgaag tttgccacgt cccgctgatt gcttccggtg gcgcgggcac
    20221 catggaacac ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc
    20281 cgtattccac aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg
    20341 cgtggagatc aggatatgtt aacagaacaa caacgtcgcg aactggactg ggaaaaaacc
    20401 gacggactga tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc
    20461 tatatgaacc cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg
    20521 cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caacgtagta
    20581 aatattgcgc cggactgcga caacgacacg ctactggtac tggcgaatcc tatcggacca
    20641 acctgccaca aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg
    20701 tatcaactgg aacaattgct cgccgaacgc aaatctgccg acccggaaac ctcctacacc
    20761 gccaaactgt atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg
    20821 gaaaccgcac tggccgcgac tgtacatgac cgctttgagc tgaccaacga ggcgtctgac
    20881 ttgatgtatc acctgctggt attgttgcag gatcaggatc tggatttaac aacggtgatt
    20941 gagaacctgc gtaaacgtca tcagtgagtt gcggggtaag cggatgcgat attgttgtcg
    21001 catccggcaa aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
    21061 agattacttc gcgttgtaat tacgcagagc gttacgcccc agcacaatcc ccgcgccaac
    21121 catgccgccc agcagcaccg ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg
    21181 aataggtaac gtcggtttca ttacatagcg gtaagcatga atatcaagat catcaacctt
    21241 taagttttca atatccaaca ggttttgacg tgtctgatag tagtttggtg agaacaccaa
    21301 cggacgggtc gcctcatgct taatcatcga ctccagcgct tcgctcccca aaaggaacaa
    21361 cgtatcttgc gtcacatctt cagtctgctg aacctgtggc tttgccacct gcgcctgatt
    21421 cgcatactgc aacgcttcct gaatctgacg gatacgcagc tctttctgct cctgcgcaac
    21481 tacttcctgc gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcttt
    21541 gagatccttt tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa
    21601 cttcatttgt gctccctctg cagtttgccc aacataagaa acagtcaatg gtaattgctg
    21661 gttcttaaca gaaggttcga tggtaagttt ttctggttct tcctgattat ccagcgtttc
    21721 tgctaatgct gagaaggcag aactgaagcg accaatcaac gtctcctgca aatccgatac
    21781 tttcggtgca gcctgaccat agataacatt catggcattg ttatagccag caatttgccc
    21841 cacgtcgggc tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata
    21901 accaatagcc agagcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca
    21961 caactgcact agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac
    22021 attattattt tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt
    22081 ttagcgataa tccctgtgaa tgctatagga taagtgataa aaataatgaa ttattgcgga
    22141 ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa
    22201 agagatcgcg ggtgtagacc ttatctgcca catccctaag ctcttctgcc atacggttgg
    22261 aaataatgac gtcggcttgt tgtttgaagg tggcgagatc acgttccagg cgagagttga
    22321 agaatgagtc ttctttcatc actggctcgt agatgattac ttcaacacct ttcgccttga
    22381 tacgcttcat aatcccctga atagaagacg cacggaagtt atctgaaccg ctcttcataa
    22441 tcagacgata aatacctacc acttgcggct tgcgtgacaa aatggcatcg gcaataaaat
    22501 ctttacgcgt gcggttagca tcgacaattg ccgagatcag gttattcggc acagactggt
    22561 agttcgccag taactgcttg gtatctttcg gcaaacaata accaccataa ccaaacgacg
    22621 gattgttgta atggttgcca atacgtgggt cgagacaaac gccttcgatt atttgacggg
    22681 aattcagacc taaactttct gcgtagctat ccagttcgtt aaagtacgcc acgcgcatcg
    22741 cgaggtaggt gtttgcaaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg
    22801 ttgggatatt ttgcttaatc gcgccttcct gtaataacgc agcaaaacgt tctgcgcgtt
    22861 ccgaacgctc accgattaca atacgtgacg gatgaaggtt atcgtaaaga gctttacctt
    22921 cgcggagaaa ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctt
    22981 cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa
    23041 ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat
    23101 agtcggttgg agtggcgatg atgacataat cagcatcccg gtaggcttca tttttatcta
    23161 atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa
    23221 taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa
    23281 cctcatgatt ttgagcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa
    23341 tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttaac
    23401 atcctgataa gagctaaaag ttttaacgct acgttgttcg atggtcaacg cagcgaaaaa
    23461 caattcagat tttctttata agaatagctg atatttatta agttaatatt aagcagtgat
    23521 atttagtcta taagataatt ggcaaaaaaa agcccggtac aatatacgcc gggcctcaat
    23581 tttattgttg attaaatcag attaatccag ccattcggta tggaacacac cttctttatc
    23641 aatgcgctta taagtatgcg caccgaaata gtcacgctgt gcctggatca ggttcgcagg
    23701 cagaacagcg gcgcggtagc tgtcataata ggcaaccgca gcggcgaagg tcggaaccgg
    23761 gataccgttc tgtaccgcat aagcgacgac atcgcgcagc gcctgctggt agtcatcggc
    23821 aatttgcttg aagtaaggag ccagcagcag gttagcgatc tgcggatttt cggcataagc
    23881 atcggtgatt ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc
    23941 gatttcgccg tagttcagat cccagttgta ctcttcagac gccgcacgca gttgagagaa
    24001 cccctgagcg taagaaacga ttttgcccag atacagtgca cggcgaactt tttcgatgaa
    24061 ctcagccttg tcgccagctg gctgcgcttg tgggccagag agaactttag acgcagcaac
    24121 gcgctgatct ttcagagaag agatgtatcg tgcaaacaca gactcggtaa tcagcgacag
    24181 cggttcgccg agatccagtg cgctctggct ggtccatttg cccgtacctt tgtttgccgc
    24241 ttcatccagg atcacatcaa ccaggtagtt accgtcttca tcttttttag tgaagatgtc
    24301 tttggtaatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg taaagatctg
    24361 cgccagttct tcgttggtga ggttcagacc acctttaagc agagaatagg cttcagcaat
    24421 cagctgcata tcgccgtatt caataccgtt gtgaaccatc ttcacatagt gacctgcgcc
    24481 atcggcacca atataggtaa cgcatggctc accgtcttca gccactgcgg cgattttggt
    24541 caggatcggc gcaactagtt cataggcttc tttctgccca ccaggcataa tggaaggacc
    24601 ttttagtgcg ccctcctcac caccggaaac accggtaccg atgaagttaa agccttctgc
    24661 agaaagctca cggttacgac gaatggtgtc ctggaagaag gtattaccac catcaatgat
    24721 gatgtcacct ttatcgaggt atggcttaag ggaatcaata gcagcatccg tgcctgcacc
    24781 tgctttcacc attaacaaga tgcgacgagg cgtttccaga gattcaacaa attctttcac
    24841 cgtatagtaa ggaaccagtt tcttgcctgg attttcggca atcacttctt ccgtcttttc
    24901 acgggaacgg ttgaaaatag agacggtata accacggctt tcgatgttga gcgcaaggtt
    24961 gcgccccatc actgccatac cgactacgcc gatctgttgc tttgacattg tttactcctg
    25021 tcaggggtta ccgctagata tgcggcttga ataacgtata aaaatgtatc agagtattat
    25081 aaatttcatg ctgtatcagt aataactaag taaaggatag cttaaaaaga agccataatt
    25141 tagtaaatta gcgaaattat atatatttta aacattagtc ccatttggaa cgtgttttcg
    25201 cattcccatt aaatagatgt tggcgatctt ttcatttctc ttgctggatt tccgcacaca
    25261 gttataccgg caggaacact tttagtgaca acagccccca tacctataat ggctcccgcg
    25321 ccaataataa gtgggcgatt aggaacaccc tgcttaatta ctgcacccga gcctatatat
    25381 gcattgtctt caataacaac atatccatta catttagccc caggagcaaa tgtaacatag
    25441 tctcctattt gacaatcatg tgcaacgtat gagtatatgt ttgcatgaaa aaacctccct
    25501 atatgagtat tagtagatat tgtaacaaag ggagaaataa tagcgccact acctatcata
    25561 gtatgatcat aaacaacgct atttggatgt tttatagtta ttggttcaac cccgtgtaat
    25621 aatattgact cagacactct ctgtcgtatc ttatcattag caatagcaac attaaaatac
    25681 ttttttaaat aaggggcttt tagaaagcag ttggttgaaa gcacttcaaa accattaact
    25741 ttctttcctg ccaaaacatc atccacaaaa accagagcat agtcagaacc acattcttgc
    25801 tttatttgtt gatttagaat gggtattgtt tctctaccat aacttccagc accaaaaata
    25861 ccatacaaat tcattttccc atctccaaag aaatatctta tcaaaaccaa tgcaagatta
    25921 tgaaagcccg cacgctgcaa tgcctgacgc taggaagttt ctgtccatga ctgcttttcg
    25981 catcttacat gataatcctg ccttgaatac attatcattg tcagatatta atagcgatgt
    26041 gccgcattct agaaaatcat tcgaatgcaa ctcgccttct tatattatag tcgatttcat
    26101 tcattaccac atcaggctac tgtgcaactt attctccatg cgccagtgat ttcgatttac
    26161 tgtggcgaac ttctctgcgg ttaaagcagc agatctaata taatatttga tcgtcatttc
    26221 ggattctttc ttttgctctg ctattattga gagaaagtgg actgccattc ataaattctg
    26281 cagccctttc cattcaaacg tgaaatcaat aagctcatca ggagcatctc aaacaatatg
    26341 aagacggaca tcgtctctgc cgtgcctctt ttcactaatt gcgtaactgt catgttttgg
    26401 attatttaat tctttcagcg taaatatctc ctcaaagact ctattaagcc gactcttatt
    26461 tccttttaca gcgaataaat aatcacatct ctgtttttat atcttctctg caatatcttt
    26521 ctggcaagcc atcgcatcag ttatgattat ttttccttta atacccatca ttttagaaag
    26581 ttcatgaata actgtgaacg cattaggttt ctcatccgtc ttgatctatc cgaggaccag
    26641 actgtgcatt gctgaaacgc actaatgaca tgaaccactc ctctgcgacg actcttgtca
    26701 taagtgtgcc gaagtgtttt accatcaatt gcgatgacgt cgttatcatc tggaaaatgg
    26761 cagacatgta ttcagttaac aaagcactcg tgaaatattg caggactgat gcaggataca
    26821 actttggcta taatatcgta aacagggata ataaaaatct attg
//
LOCUS       DAZGXS010000059        26367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGXS010000059 DAZGXS010000000
VERSION     DAZGXS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            137..646
                     /locus_tag="ACHKG5_002549"
     CDS             137..646
                     /locus_tag="ACHKG5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0868631.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHKG5_002550"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHKG5_002550"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0868632.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKG5_002551"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKG5_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0868633.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKG5_002552"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKG5_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0868634.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKG5_002553"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKG5_002553"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0868635.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKG5_002554"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKG5_002554"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0868636.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKG5_002555"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKG5_002555"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0868637.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHKG5_002556"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHKG5_002556"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0868638.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /locus_tag="ACHKG5_002557"
     CDS             complement(7859..8905)
                     /locus_tag="ACHKG5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0868639.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /locus_tag="ACHKG5_002558"
     CDS             complement(9054..10115)
                     /locus_tag="ACHKG5_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0868640.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHKG5_002559"
     CDS             complement(10112..10843)
                     /locus_tag="ACHKG5_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0868641.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHKG5_002560"
     CDS             complement(10858..13317)
                     /locus_tag="ACHKG5_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0868642.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13367..13933)
                     /locus_tag="ACHKG5_002561"
     CDS             complement(13367..13933)
                     /locus_tag="ACHKG5_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0868643.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHKG5_002562"
     CDS             complement(14320..15603)
                     /gene="gltA"
                     /locus_tag="ACHKG5_002562"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0868644.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15809..15916)
                     /locus_tag="ACHKG5_002563"
     CDS             complement(15809..15916)
                     /locus_tag="ACHKG5_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868645.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHKG5_002564"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16297..16701
                     /gene="sdhC"
                     /locus_tag="ACHKG5_002564"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0868646.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHKG5_002565"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16695..17042
                     /gene="sdhD"
                     /locus_tag="ACHKG5_002565"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0868647.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHKG5_002566"
     CDS             17042..18808
                     /gene="sdhA"
                     /locus_tag="ACHKG5_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0868648.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHKG5_002567"
     CDS             18824..19540
                     /gene="sdhB"
                     /locus_tag="ACHKG5_002567"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0868649.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19841..22642
                     /gene="sucA"
                     /locus_tag="ACHKG5_002568"
     CDS             19841..22642
                     /gene="sucA"
                     /locus_tag="ACHKG5_002568"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0868650.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22657..23874
                     /gene="odhB"
                     /locus_tag="ACHKG5_002569"
     CDS             22657..23874
                     /gene="odhB"
                     /locus_tag="ACHKG5_002569"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0868651.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            23968..25134
                     /gene="sucC"
                     /locus_tag="ACHKG5_002570"
     CDS             23968..25134
                     /gene="sucC"
                     /locus_tag="ACHKG5_002570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0868652.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25134..26003
                     /gene="sucD"
                     /locus_tag="ACHKG5_002571"
     CDS             25134..26003
                     /gene="sucD"
                     /locus_tag="ACHKG5_002571"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0868653.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6382 a         6593 c         7186 g         6206 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccatcgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc gggtttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggcggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tccttcatgg gctatgataa agtatactaa ttattccgac
    13981 agaatagtgg ttttttgttc atgtaataat attaatgttg ctacattatg tttgagagtt
    14041 tctcaaagct ctcgaatcat aaataaagtg aattaatcct attacgagtt tatttaattg
    14101 tgggtgctac ccaatgtatt ctacatcgat tggcgggata acggtggtgg tgaatgaaaa
    14161 attaggattt tatttagtta tcggatatgt attcttgtaa cgtgtaatta tgtatttgtg
    14221 tccgattacg tttttttgtc gatagacata actaacgaac aaaaaaaagc ccgccagata
    14281 ttcaggcggg ctaaacgata tatggttcaa atcagataat taatgtttaa catctgattt
    14341 aaagtctcgt ttttcatagc cggtgtacaa ctggcgcgga cgggcgatct tcatgccgtc
    14401 actgtgcatt tcgctccagt gagcgatcca gccaacggta cgggccatag cgaagatgac
    14461 ggtgaacatg gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac
    14521 gttcgggtac agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc
    14581 catagccact tccagcaggt catctttggt gcccagctct ttcagaactt catggcaggt
    14641 ttcacgcatt acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat
    14701 caggcggaaa gaatcatttt tatctttcgc acgacgaaca aattccggaa tgtgtttaac
    14761 ggagctaatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gtgcaggccc
    14821 ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc
    14881 ggtacgcacg gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg
    14941 gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat
    15001 catattcagg aagttaccgg cataggagag atcgttgcgc ggataaacaa atggctgacc
    15061 aatggaatac ttgtaacaca tcgctgccat ggtcggcatt ttcgacagca ggcggaacgc
    15121 ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga acgccgccag
    15181 cgcgccggta ataccacaca tgactgccat tggatgtgag tcgcgacgga aagcgtggaa
    15241 cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc
    15301 atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt
    15361 agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc
    15421 atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagccag ggtcaaaggt
    15481 gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt
    15541 cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc
    15601 catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg
    15661 cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa
    15721 acctgggtac agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg
    15781 cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata actgtcccga
    15841 atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt
    15901 cataactttt acgcattata tgcttttcct ggtaatgttt gtaacaactt tgttgaatga
    15961 ttgtcaaatt agatgattaa aaattaaata aatgttgtta tcgtgacctg gatcactgtt
    16021 caggataaaa cccgacaaac tatatgtagg ttaattgtaa tgattttgtg aacggcctat
    16081 actgccgcca ggtctccgga acaccctgca atcccgagcc acccagcgtt gtaacgtgtc
    16141 gttttcgcat ctggaagcag tgttttgcat gacgcgcagt tatagaaagg acgctgtctg
    16201 acccgcaagc agaccggagg aaggaaatcc cgacgtctcc aggtaacaga aagttaacct
    16261 ctgtgcccgt agtccccagg gaataataag aacagcatgt gggcgttatt catgataaga
    16321 aatgtgaaaa aacaaagacc tgttaatctg gacctacaga ccatccggtt ccccatcacg
    16381 gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc agtgggtatc
    16441 ctgctgtggc ttctgggtac cagcctctct tcccctgaag gtttcgagca agcttccgcg
    16501 attatgggca gcttcttcgt caaatttatc atgtggggca tccttaccgc tctggcgtat
    16561 cacgtcgtcg taggtattcg ccacatgatg atggattttg gctatctgga agaaacattc
    16621 gaagcgggta aacgctccgc caaaatctcc tttgttatta ctgtcgtgct ttcacttctc
    16681 gcaggagtcc tcgtatggta agcaacgcct ccgcattagg acgcaatggc gtacatgatt
    16741 tcatcctcgt tcgcgctacc gctatcgtcc tgacgctcta catcatttat atggtcggtt
    16801 ttttcgccac cagtggcgag ctgacatatg aagtctggat cggtttctgc gcctctgcgt
    16861 tcaccaaagt gttcaccctg ctggcgctgt tttctatctt gatccatgcc tggatcggca
    16921 tgtggcaggt gttgaccgac tacgttaaac cgctggcttt gcgcctgatg ctgcaactgg
    16981 tgattgtcgt tgcactggtg gtttacgtga tttatggatt cgttgtggtg tggggtgtgt
    17041 gatgaaattg ccagtcagag aatttgatgc agttgtgatt ggtgccggcg gcgcaggtat
    17101 gcgcgcggcg ctgcaaattt cccagagcgg ccagacctgt gcgctgctct ctaaagtctt
    17161 cccgacccgt tcccataccg tttctgcgca aggcggcatt accgttgcgc tgggtaatac
    17221 ccatgaagat aactgggaat ggcatatgta cgacaccgtg aaagggtcgg actatatcgg
    17281 tgaccaggac gcgattgaat atatgtgtaa aaccgggccg gaagcgattc tggaactcga
    17341 acacatgggc ctgccgttct cgcgtctcga tgatggtcgt atctatcaac gtccgtttgg
    17401 cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgc actgcggcag cggctgaccg
    17461 taccggtcac gcactgttgc acacgcttta tcagcagaac ctgaaaaacc acaccaccat
    17521 tttctccgag tggtatgcgc tggatctggt gaaaaaccag gatggcgcgg tggtgggttg
    17581 taccgcactg tgcatcgaaa ccggtgaagt ggtttatttt aaagcccgcg ctaccgtgct
    17641 ggcgactggc ggagcagggc gtatttatca gtctaccact aacgcccaca ttaacaccgg
    17701 cgacggcgtc ggcatggcta tccgcgcagg cgtaccggtg caggatatgg aaatgtggca
    17761 attccacccg accggcattg ccggtgcggg cgtactggtc accgaaggtt gccgtggtga
    17821 aggcggttat ctgctgaaca aacatggcga acgctttatg gagcgttatg cgccgaacgc
    17881 caaagacctg gcgggccgtg atgtggtggc gcgttcaatc atgattgaaa tccgtgaagg
    17941 tcgcggctgt gatggtccgt gggggccaca cgcgaaactg aaactcgatc acctgggtaa
    18001 agaagttctc gaatcccgtc tgccgggtat cctggagctt tcccgtacct tcgctcacgt
    18061 tgatccggtg aaagagccga ttccggttat cccaacctgt cactacatga tgggcggtat
    18121 tccgaccaaa gttaccggtc aggcgctgac tgtgaatgag aaaggcgaag atgtggttgt
    18181 tccggggctg ttcgcggtag gtgaaattgc ctgcgtatcg gtacacggtg ctaaccgtct
    18241 gggcggcaac tcgctgctgg acctggtggt ctttggtcgc gcagcgggtc tgcatctgca
    18301 agagtctatc gccgagcagg gcgcattgcg cgatgccagc gagtctgatg ttgaagcatc
    18361 tttggatcgt ctgaaccgct ggaacaataa tcgtaacggt gaagatccgg tggcgattcg
    18421 taaagcgctg caagaatgta tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat
    18481 ggctaaaggg cttgagcagt tgaaagtgat ccgcgagcgt ctgaaaaatg cccgtctgga
    18541 tgacacttcc agcgagttca acacccagcg cgttgagtgc ctggaactgg ataacctgat
    18601 ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt accgaaagcc gtggcgcaca
    18661 tagccgcttc gacttcccgg atcgtgatga tgaaaactgg ctgtgccact ccctgtatct
    18721 gccagagtcg gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc
    18781 attcccgccg aagattcgta cttactaatg cggagacagg aaaatgagac tcgagttttc
    18841 aatttatcgc tataacccgg atgttgatga tgctccgcgt atgcaggatt acaccctgga
    18901 agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt atccagctga aagagaaaga
    18961 tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa
    19021 catgaacggc aagaatggtc tggcctgtat taccccgatt tcggcactca accagccggg
    19081 caagaagatt gtgattcgcc cgctgccagg tttaccggtg atccgcgatt tggtggtaga
    19141 catgggacaa ttctatgcgc aatatgagaa aattaagcct tacctgttga ataatggaca
    19201 aaatccgcca gctcgcgagc atttacagat gccagagcag cgcgaaaaac tcgacgggct
    19261 gtatgaatgt attctctgcg catgttgttc aacctcttgt ccgtctttct ggtggaatcc
    19321 cgataagttt atcggcccgg caggcttgtt agcggcatat cgtttcctga tcgatagccg
    19381 tgataccgag actgacagcc gcctcgacgg tttgagcgat gcattcagtg tattccgctg
    19441 tcatagcatc atgaactgcg tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat
    19501 cggccatatc aagtcgatgt tgttgcaacg taatgcgtaa accgtaggcc tgataagacg
    19561 cgcaagcgtc gcatcaggca accagtgccg gatgcggcgt gaacgcctta tccggcctac
    19621 aagtcattac ccgtaggcct gataagcgca gcgcatcagg cgtaacaaag aaatgcagga
    19681 aatctttaaa aactgcccct gacactaaga cagtttttaa aggttccttc gcgagccact
    19741 acgtagacaa gagctcgcaa gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc
    19801 acggcgaagt aagcataaaa aagatgctta agggatcacg atgcagaaca gcgctttgaa
    19861 agcctggttg gactcttctt acctctctgg cgcaaaccag agctggatag aacagctcta
    19921 tgaagacttc ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca
    19981 gttacctggt acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg
    20041 ccgcctggcg aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt
    20101 gaagcaggta aaagtcctgc agctcattaa cgcataccgc ttccgtggtc accagcatgc
    20161 gaatctcgat ccgctgggac tgtggcagca agataaagtg gccgatctgg atccgtcttt
    20221 ccacgatctg accgaagcag acttccagga gaccttcaac gtcggttcat ttgccagcgg
    20281 caaagaaacc atgaaactcg gcgagctgct ggaagccctc aagcaaacct actgcggccc
    20341 gattggtgcc gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg
    20401 tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttcc taagcgaact
    20461 gaccgccgct gaaggccttg aacgttacct cggtgcaaaa ttccctggcg caaaacgctt
    20521 ctcgctggaa ggcggtgacg cgttaatccc gatgcttaaa gagatgattc gccacgctgg
    20581 caacagcggc acccgcgaag tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct
    20641 ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac gagtttgccg gtaaacataa
    20701 agaacacctc ggcacgggtg acgtgaaata ccacatgggc ttctcgtctg acttccagac
    20761 cgatggcggc ctggtacatc tggcgctggc gtttaacccg tctcaccttg agattgtcag
    20821 cccggtcgtt atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa
    20881 caaagtgctg ccaatcacca ttcacggtga cgccgcagtg accgggcagg gcgtggttca
    20941 ggaaactctg aacatgtcga aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt
    21001 tatcaacaac caggttggct tcaccacctc taacccgctg gatgcccgtt caacgccgta
    21061 ctgtactgat atcggtaaga tggttcaggc accgattttt cacgttaacg cggatgatcc
    21121 ggaagccgtt gcctttgtga cccgtctggc gctcgatttc cgtaacacct ttaaacgtga
    21181 tgtcttcatc gacctggtgt gctaccgccg tcacggccac aacgaagccg acgagccgag
    21241 cgcaacccag ccgctgatgt atcagaaaat caaaaaacat ccgacgccgc gcaaaatcta
    21301 tgctgacaag ctggagcagg aaaaagtggc gacgctggaa gatgccaccg agatggttaa
    21361 cctgtaccgc gatgcgctgg atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa
    21421 catgcactct ttcacctggt cgccgtacct caaccacgaa tgggacgaag agtacccgaa
    21481 taaagttgag atgaagcgcc tgcaggagct ggcgaaacgc atcagcacgg tgccggaagc
    21541 ggttgaaatg cagtctcgcg ttgccaaaat ttatggcgat cgccaggcga tggctgccgg
    21601 tgagaaactg ttcgactggg gcggcgcgga aaacctcgct tacgccacgc tggttgacga
    21661 aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca
    21721 cgcggtgatc cacaaccagt ctaacggttc cacttacacg ccgctgcaac acatccataa
    21781 cggccagggc gcgttccgtg tctgggactc cgtactttct gaagaagcag tgctggcgtt
    21841 tgaatacggt tatgccaccg cagaaccacg cactctgact atctgggaag ctcagttcgg
    21901 tgacttcgcc aacggtgcgc aggtggttat cgaccagttc atctcctctg gcgaacagaa
    21961 atggggccgg atgtgtggtc tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc
    22021 ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt tgtgctgagc aaaacatgca
    22081 ggtgtgcgta ccgtctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg
    22141 cgggatgcgt cgtccgctgg tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc
    22201 ggtttccagc ctcgaagaac tggcgaacgg caccttcctg ccagccatcg gtgaaatcga
    22261 cgagcttgat ccgaagggcg tgaagcgcgt agtgatgtgt tctggtaagg tttattacga
    22321 cctgctggaa cagcgtcgta agaacaatca acacgatgtc gccattgtgc gtatcgagca
    22381 actctacccg ttcccgcata aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa
    22441 ggattttgtc tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca
    22501 tcatttccgt gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc
    22561 cgcctctccg gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa
    22621 tgacgcgctg aacgtcgaat aaataaagga tacacaatga gtagcgtaga tattctggtc
    22681 cctgacctgc ctgaatccgt agccgatgcc accgtcgcaa cctggcataa aaaacccggt
    22741 gacgcagtcg tacgtgatga agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa
    22801 gtaccggcat cagcagacgg cattctggat gcggttctgg aagatgaagg tacaacggta
    22861 acgtctcgtc agatccttgg tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc
    22921 gccaaatctg aagagaaagc gtccactccg gcgcaacgcc agcaggcgtc tctggaagag
    22981 caaaacaacg atgcgttaag cccggcgatc cgtcgcctgc tggctgaaca taacctcgac
    23041 gccagcgcca ttaaaggcac cggtgtgggt ggtcgtctga cccgtgaaga tgtggaaaaa
    23101 catctggcga aagctccggc gaaagagtct gcaccggcag cggctgctcc ggcggcgcaa
    23161 ccggctctgg ctgcacgtag cgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg
    23221 gcagagcgtc tgctggaagc gaaaaactcc accgccatgc tgaccacgtt caacgaagtc
    23281 aacatgaagc cgattatgga tctgcgtaag cagtacggtg aagcgtttga aaaacgccac
    23341 ggcatccgtc tgggctttat gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt
    23401 tacccggaag tgaatgcttc tatcgacggc gatgacgtgg tttaccacaa ctatttcgac
    23461 gtcagcatgg cggtttctac gccgcgcggc ctggtgacgc cggtactgcg tgatgtcgat
    23521 accctcggca tggcagacat cgagaagaaa atcaaagagc tggcagttaa aggccgtgac
    23581 ggcaagctga cggttgaaga tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg
    23641 ttcggttccc tgatgtctac gccgatcatc aacccgccgc agagcgcaat tctgggtatg
    23701 cacgctatca aagatcgtcc gatggctgtg aatggtcagg ttgagatcct gccgatgatg
    23761 tacctggcgc tgtcctacga tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg
    23821 gtaacgatca aagagttgct ggaagatccg acgcgtctgc tgctggacgt gtagtagttt
    23881 aagtttcacc tgcactgtag accgacgtgg atagggtgtc ggtctacggt ttaaaagata
    23941 acgattactg aaggatggac agaacacatg aacttacatg aatatcaggc aaaacaactg
    24001 tttgcccgct atggcttacc agcaccggtg ggttatgcct gtactactcc gcgcgaagca
    24061 gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag tgaaatgtca ggttcacgct
    24121 ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct
    24181 tttgcagaaa actggctggg caagcgtctg gtaacgtatc aaacagatgc caatggccaa
    24241 ccggttaacc agattctggt tgaagcagcg accgatatcg ctaaagagct gtatctcggt
    24301 gccgttgtag accgtagttc acgtcgcgtg gtctttatgg cctccaccga aggcggcgtg
    24361 gaaatcgaaa aagtggcgga agaaactccg cacctgatcc acaaagttgc gcttgatccg
    24421 ctgactggcc cgatgccgta tcagggacgc gagctggcgt tcaaactggg tctggaaggt
    24481 aaactggttc agcagttcac caaaatcttc atgggcctgg cgaccatttt cctggagcgc
    24541 gacctggcgc tgatcgaaat caacccgctg gtcatcacca aacagggcga tctgatttgc
    24601 ctcgacggca aactgggcgc tgatggcaac gccctgttcc gccagcctga tctgcgcgaa
    24661 atgcgtgacc agtcgcagga agatccgcgt gaagcacagg ctgcacagtg ggaactgaac
    24721 tacgttgcgc tggacggtaa catcggttgt atggttaacg gcgcaggcct ggcgatgggt
    24781 acgatggaca tcgttaaact gcacggcggc gaaccggcta acttcctgga cgttggcggc
    24841 ggcgcaacca aagaacgtgt aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg
    24901 aaagccgttc tggttaacat cttcggcggt atcgttcgtt gcgacctgat cgctgacggt
    24961 atcatcggtg cggtagcgga agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt
    25021 aacaacgccg aactcggcgc gaagaaactg gctgacagcg gcctgaatat tattgcagca
    25081 aaaggtctga cggatgcagc tcagcaggtt gttgccgcag tggaggggaa ataatgtcca
    25141 ttttaattga taaaaacacc aaggttatct gccagggctt taccggtagc caggggactt
    25201 tccactcaga acaggccatt gcatacggca ctaaaatggt tggcggcgta accccaggta
    25261 aaggcggtac tacccacctc ggcctgccgg tgttcaacac cgtgcgtgaa gctgttgctg
    25321 ctactggcgc taccgcttct gttatctacg taccagcacc gttctgcaaa gactccattc
    25381 tggaagccat cgacgcaggc atcaaactga ttatcaccat cactgaaggc atcccgacgc
    25441 tggatatgct gaccgtgaaa gtgaagctgg atgaagcagg cgttcgtatg atcggcccga
    25501 actgcccagg cgttatcact ccgggtgaat gcaaaatcgg tattcagcct ggtcacattc
    25561 acaaaccggg taaagtgggt atcgtttccc gttccggtac actgacctat gaagcggtta
    25621 aacagaccac ggattacggt ttcggtcagt cgacctgtgt cggtatcggc ggtgacccga
    25681 tccctggctc taactttatc gacattctcg aaatgttcga aaaagatccg cagaccgaag
    25741 cgatcgtgat gatcggtgag atcggcggta gcgctgaaga agaagcagct gcgtacatca
    25801 aagagcacgt taccaagcca gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca
    25861 aacgtatggg ccacgcgggt gccatcattg ccggtgggaa agggactgcg gatgagaaat
    25921 tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag cctggcggat atcggtgaag
    25981 cactgaaaac tgttctgaaa taaatatctg taataagaaa tagccctcgc cgcttccctc
    26041 tacaggaatg gcgaagggct gtcggtttcg acatggttgg ccatcgtatg atggcctttt
    26101 ttatgcctta aacggttaac cacggtcgaa atctgactgc aggcttgact gcatgaccac
    26161 agccactgtg gattgccgca accgaccgca agccacgaat aagaacgacg gcgaatttcg
    26221 aatcgttacc tgatgcactg gcttcccagc gtttgaccgt agcgttaagt aaatcaacaa
    26281 aagcgatgtt caccgctttt cgtcaagtcg tgccatttgt cagccaggat cgggtcttta
    26341 ccccggatat tgcagcatcg caaaaat
//
LOCUS       DAZGXS010000060        26068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGXS010000060 DAZGXS010000000
VERSION     DAZGXS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHKG5_002572"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHKG5_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0868654.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHKG5_002573"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHKG5_002573"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0868655.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHKG5_002574"
     CDS             complement(938..1405)
                     /locus_tag="ACHKG5_002574"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0868656.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKG5_002575"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKG5_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0868657.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKG5_002576"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKG5_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0868658.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKG5_002577"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKG5_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0868659.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKG5_002578"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKG5_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0868660.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKG5_002579"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKG5_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0868661.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKG5_002580"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKG5_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0868662.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKG5_002581"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKG5_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0868663.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKG5_002582"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKG5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0868664.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKG5_002583"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKG5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0868665.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKG5_002584"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKG5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0868666.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKG5_002585"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKG5_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0868667.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKG5_002586"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKG5_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0868668.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKG5_002587"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKG5_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0868669.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKG5_002588"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKG5_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0868670.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKG5_002589"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKG5_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0868671.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKG5_002590"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKG5_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0868672.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKG5_002591"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKG5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0868673.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKG5_002592"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKG5_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0868674.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKG5_002593"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKG5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0868675.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKG5_002594"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKG5_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0868676.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKG5_002595"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKG5_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0868677.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHKG5_002596"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHKG5_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0868678.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKG5_002597"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKG5_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0868679.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKG5_002598"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKG5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0868680.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKG5_002599"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKG5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0868681.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKG5_002600"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKG5_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0868682.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKG5_002601"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKG5_002601"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0868683.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKG5_002602"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKG5_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0868684.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHKG5_002603"
     CDS             15545..15913
                     /locus_tag="ACHKG5_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0868685.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKG5_002604"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKG5_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0868686.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKG5_002605"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKG5_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0868687.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKG5_002606"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKG5_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0868688.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKG5_002607"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKG5_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0868689.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKG5_002608"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKG5_002608"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0868690.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKG5_002609"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKG5_002609"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0868691.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKG5_002610"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKG5_002610"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0868692.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHKG5_002611"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHKG5_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP0868693.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFSLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHKG5_002612"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHKG5_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0868694.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHKG5_002613"
     CDS             23092..23634
                     /locus_tag="ACHKG5_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0868695.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKG5_002614"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKG5_002614"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0868696.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKG5_002615"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKG5_002615"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0868697.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHKG5_002616"
     CDS             25031..25288
                     /locus_tag="ACHKG5_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0868698.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHKG5_002617"
     CDS             complement(25264..25818)
                     /locus_tag="ACHKG5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0868699.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6689 a         6177 c         6762 g         6440 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 attttccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZGXS010000061        25949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGXS010000061 DAZGXS010000000
VERSION     DAZGXS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(79..159)
                     /gene="shoB"
                     /locus_tag="ACHKG5_002618"
     CDS             complement(79..159)
                     /gene="shoB"
                     /locus_tag="ACHKG5_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0868700.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(581..961)
                     /gene="acpS"
                     /locus_tag="ACHKG5_002619"
     CDS             complement(581..961)
                     /gene="acpS"
                     /locus_tag="ACHKG5_002619"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0868701.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(961..1692)
                     /gene="pdxJ"
                     /locus_tag="ACHKG5_002620"
     CDS             complement(961..1692)
                     /gene="pdxJ"
                     /locus_tag="ACHKG5_002620"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0868702.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQVGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1704..2432)
                     /gene="recO"
                     /locus_tag="ACHKG5_002621"
     CDS             complement(1704..2432)
                     /gene="recO"
                     /locus_tag="ACHKG5_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0868703.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2444..3349)
                     /gene="era"
                     /locus_tag="ACHKG5_002622"
     CDS             complement(2444..3349)
                     /gene="era"
                     /locus_tag="ACHKG5_002622"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0868704.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3346..4026)
                     /gene="rnc"
                     /locus_tag="ACHKG5_002623"
     CDS             complement(3346..4026)
                     /gene="rnc"
                     /locus_tag="ACHKG5_002623"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0868705.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4299..5273)
                     /gene="lepB"
                     /locus_tag="ACHKG5_002624"
     CDS             complement(4299..5273)
                     /gene="lepB"
                     /locus_tag="ACHKG5_002624"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0868706.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5289..7088)
                     /gene="lepA"
                     /locus_tag="ACHKG5_002625"
     CDS             complement(5289..7088)
                     /gene="lepA"
                     /locus_tag="ACHKG5_002625"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0868707.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7286..7765)
                     /gene="rseC"
                     /locus_tag="ACHKG5_002626"
     CDS             complement(7286..7765)
                     /gene="rseC"
                     /locus_tag="ACHKG5_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0868708.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7762..8718)
                     /gene="rseB"
                     /locus_tag="ACHKG5_002627"
     CDS             complement(7762..8718)
                     /gene="rseB"
                     /locus_tag="ACHKG5_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0868709.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8718..9356)
                     /gene="rseA"
                     /locus_tag="ACHKG5_002628"
     CDS             complement(8718..9356)
                     /gene="rseA"
                     /locus_tag="ACHKG5_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0868710.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9389..9964)
                     /gene="rpoE"
                     /locus_tag="ACHKG5_002629"
     CDS             complement(9389..9964)
                     /gene="rpoE"
                     /locus_tag="ACHKG5_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0868711.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9961..10116)
                     /gene="rseD"
                     /locus_tag="ACHKG5_002630"
     CDS             complement(9961..10116)
                     /gene="rseD"
                     /locus_tag="ACHKG5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0868712.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10372..11994
                     /gene="nadB"
                     /locus_tag="ACHKG5_002631"
     CDS             10372..11994
                     /gene="nadB"
                     /locus_tag="ACHKG5_002631"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0868713.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11979..12716)
                     /gene="trmN"
                     /locus_tag="ACHKG5_002632"
     CDS             complement(11979..12716)
                     /gene="trmN"
                     /locus_tag="ACHKG5_002632"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0868714.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12848..14182
                     /gene="srmB"
                     /locus_tag="ACHKG5_002633"
     CDS             12848..14182
                     /gene="srmB"
                     /locus_tag="ACHKG5_002633"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0868715.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14215..15096)
                     /gene="yfiE"
                     /locus_tag="ACHKG5_002634"
     CDS             complement(14215..15096)
                     /gene="yfiE"
                     /locus_tag="ACHKG5_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0868716.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15199..15786
                     /gene="eamB"
                     /locus_tag="ACHKG5_002635"
     CDS             15199..15786
                     /gene="eamB"
                     /locus_tag="ACHKG5_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0868717.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15842..16225)
                     /gene="grcA"
                     /locus_tag="ACHKG5_002636"
     CDS             complement(15842..16225)
                     /gene="grcA"
                     /locus_tag="ACHKG5_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0868718.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16530..17219
                     /gene="ung"
                     /locus_tag="ACHKG5_002637"
     CDS             16530..17219
                     /gene="ung"
                     /locus_tag="ACHKG5_002637"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0868719.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17267..18304)
                     /gene="yfiF"
                     /locus_tag="ACHKG5_002638"
     CDS             complement(17267..18304)
                     /gene="yfiF"
                     /locus_tag="ACHKG5_002638"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0868720.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18511..18930
                     /gene="trxC"
                     /locus_tag="ACHKG5_002639"
     CDS             18511..18930
                     /gene="trxC"
                     /locus_tag="ACHKG5_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0868721.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18999..19697
                     /gene="tapT"
                     /locus_tag="ACHKG5_002640"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18999..19697
                     /gene="tapT"
                     /locus_tag="ACHKG5_002640"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0868722.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19729..22389
                     /gene="pat"
                     /locus_tag="ACHKG5_002641"
     CDS             19729..22389
                     /gene="pat"
                     /locus_tag="ACHKG5_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0868723.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22503..23858
                     /gene="pssA"
                     /locus_tag="ACHKG5_002642"
     CDS             22503..23858
                     /gene="pssA"
                     /locus_tag="ACHKG5_002642"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0868724.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23904..24227
                     /gene="yfiM"
                     /locus_tag="ACHKG5_002643"
     CDS             23904..24227
                     /gene="yfiM"
                     /locus_tag="ACHKG5_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0868725.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24224..25522)
                     /gene="kgtP"
                     /locus_tag="ACHKG5_002644"
     CDS             complement(24224..25522)
                     /gene="kgtP"
                     /locus_tag="ACHKG5_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0868726.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25845..>25949)
                     /gene="rrf"
                     /locus_tag="ACHKG5_002645"
     rRNA            complement(25845..>25949)
                     /gene="rrf"
                     /locus_tag="ACHKG5_002645"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6263 a         6865 c         6489 g         6332 t
ORIGIN      
        1 tgcccagaca cttatttata gttattaaag gcgcgtccga ttggttcacc ggacgcacct
       61 taagtacgtt tccttgtgtt ataagaacag aaggatcagc tgtaaaacag caatgatgat
      121 tttgatgacc cgtttaatca ggtatcggca atcagtcatt cgtttttcct taaacaagga
      181 atgcagccgt tcagattagc ccttacatct ccccaaaact gaacgtgcga gttattggaa
      241 tcgagtgctg cactccacac cagagctttg acgacaccac tcgtttcaat gggggaattc
      301 agtggcatgg tgtaaagcac agcaaaattg ccaataacga aaccgatttt aatgtactta
      361 tcaaaaagcg gtcacgttaa tcttttcatc aatgtcgatt tcttcgtaaa ggctcgcgtt
      421 ttttatgtca gagattgcaa gttgcttgta caggtcaacg aaattgatcc ataatcattt
      481 tcgttggaat cgtgtgctgc acaaaattaa agttaaaaag taaaaccccc gttccttacc
      541 agttcggggg ttttacttta taaagagaac ggtattattt ttaactttca ataattaccg
      601 tggcacaagc atagtggcgc tcatcggcca gcgtcacatg catatttgca acgcccagct
      661 tttccgccag ttttaatgcc tcgccccaca gtcgcagccg tggcttacca agctcatcgt
      721 taaatacttc aaactgatta aacgccagac cattgcggat cccggtgcca aacgcttttg
      781 ctgcggcttc ttttacggca aagcgtttcg ccagaaaacg caccggctgg tggtgcgttt
      841 tccaaatttc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg
      901 cgatcactgc ttctatgcga gcgatctcta cgatatccgt gcctaaacct aatattgcca
      961 ttagccacgc gcttccagca tcaggcgctt catttctgcc accgcatctt tcagtccggt
     1021 catcactgca cgaccaataa tggcatgacc gatattcagt tcgtgcatct cagggatggc
     1081 ggcaatggct tttacgttgt gataggtcag accgtgtccg gcgttaactt tcaaaccgag
     1141 actagcggca aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc
     1201 agttttggca tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc
     1261 cgcagctttg atctgctctt catcggcgtc gataaacaga gaaacctgaa tcccggcatc
     1321 cgccagacgt ttgcaggcat cgcgcatttt gtcacgctgc cctgcaacgt ccaggccgcc
     1381 ttcggttgtt acttcctgac gcttttctgg taccaggcag caaaaatgcg gcttcgtctc
     1441 aaccgcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag
     1501 cgtctgacgc aggatgcgca catcgcggtc agtaatgtgg cggcgatctt cacgtaaatg
     1561 cacggtaatg ccgtccgccc ccacctgctc ggcaataaac gcggcctgca ccggatccgg
     1621 gtaagcggta ccgcgcgcgt tgcgcagcgt agcgatatgg tcaatgttga cgcctaacag
     1681 taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata
     1741 aattgccgga acagttccct gctctttaaa ggtttaccgc caagatacgg cttaagcgcc
     1801 atgcgggtaa agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc ccgtgcgttt
     1861 aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac
     1921 cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca
     1981 caatgggtaa aattgacgcc ataacccaga tgcccgagca gtgccagttc aaagcggcgc
     2041 agcgcgggtt ctggcgtacc agtgacccct gcaagagact gaatgcagtg caagtaatcg
     2101 aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg
     2161 tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca
     2221 ctgcgcagcg ttttgacttc gccacgcccg ccaaaacgta gcaagagagg ggtgaaaggc
     2281 tgtaatgcac ctttcagggt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg
     2341 cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta
     2401 tgcaggacaa atgcgcgctg ccagccttcc atcggagtta ctcttaaaga tcgtcaacgt
     2461 aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca
     2521 gctcaaggtg aacaggcgct tcaaacattt cctgcatgtc tttacgcgct tcaatcccga
     2581 tggttttgat cttggcccct ttgttgccaa tgaccatctt cttctgccct tcacgctcaa
     2641 cgagaatcaa accgttgatg tcataaccac cgcgttcgtt agagacgaaa cgttcgatct
     2701 ccacggtcac ggagtacggc agttcagcgc cgaggaaacg catcagtttt tcgcggatga
     2761 tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatgag
     2821 tcgcttcagg tagatgctta cgcacgattg ccgcaatagt ggcaacattc agcccggttt
     2881 cggcagagat tggcacgata tcgaggaagt tcatctggct tgccaggaac tgcaggtgcg
     2941 gcagcagatc ggctttctcc tgcacgttgt ccactttgtt caccgcgagg attaccggcg
     3001 ctttgccttc gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt
     3061 caacgacaaa aatcaccagc tcaacatcgc caatagagct gctcgccgct ttgttcatca
     3121 ggcggttaat agcgcgtttt tcttccatat gcaggcccgg tgtatcgacg tagatcgcct
     3181 gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtctgc gccttgcggg
     3241 aggtgatgga gattttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacattcg
     3301 gacgtccgac gatggcaata aatccgcagt aacttttatc gatgctcatt ccagctccag
     3361 ttttttcaac gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc
     3421 aaccaccggt tcactcaggc cgctgacctg gcagtggatt gtaaattcct gatcgtgcgc
     3481 ttcgccacgg acctgaacta ccagataagt cggcagcggc agatgacgac cctgcaaata
     3541 ttcttgcaag cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccagacg
     3601 agtttgatac cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac
     3661 gccaccaatt aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct
     3721 tttaagttca cctggtccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc
     3781 cgccagcgta ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac
     3841 acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa
     3901 aaattctaaa cgctcgttat gtttgctgct ggcactacga tgagttaatg cctgctgcaa
     3961 cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg
     4021 gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata
     4081 tacatggctc tgctaactgc ttcgctgcag tttcagttta gcggtatatg gggaccaacg
     4141 ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta
     4201 tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt
     4261 gtcgccataa cggcgacaac gtgaacgaag atgagctatt aatggatgcc gccaatgcga
     4321 cttaagcgca cacctgtcgg ccattcgcct tcttgcttat cgaagctcat ccagatagcc
     4381 gttgcccgac cgaccagatt cgcttcaggc acaaagcccc agtaacggct gtccgcgctg
     4441 ttgtcgcggt tgtcgcccat catgaagtat tgtcccggag gaacaatcca ggttgccagt
     4501 tgttgccctg gctgctggta atacatcccc acctgatcct gcgcaatcgg cactgtcaga
     4561 atgcggtgcg tcacatcacc cagtgtctct ttacgctcgg aaagacgaat tccattttct
     4621 ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg cttccccacc attacggcgt
     4681 gagaaggtct gaacgaaatc gctcggttcc acgtttgagt aggtgaccgg cagcgcgttt
     4741 tcacacgcct ggccggaact gcatcccggt tgaatcgtca gctcttttga gaccggatcg
     4801 taagtgactt tatcgcccgg taaacccacc gcgcgcttga tataatcaag ctttggatct
     4861 tccggatatt taaagaccac gatatcgccg cgtttcggat gaccggtttc gatcagcgtt
     4921 ttctggtaga taggatcttt aatgccataa gcaaacttct ctaccagaat aaaatcaccg
     4981 attaacagag tcggcatcat cgaacctgac ggaatctgga acggttcata aataaacgaa
     5041 cgcacaatca atacgatagc cagtaccgga aaaacagaag caccggtttc cagccagcca
     5101 ggcttcggcg caaccttttt caacgttgct ttatccagtg agtcaccggc agccgcctgc
     5161 gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga atttatccac gcaccataaa
     5221 atgcccgtca ccagtgtggc aatcaccaga atcagggcaa acatattcgc catgccaact
     5281 cctaagggtt atttgttgtc tttgccgacg tgcagaatgg cgaggaacgc ttcctgcggc
     5341 agctcgacgt taccgatctg cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc
     5401 tttttcttac ggctgatatc gccgccataa catttagcca gtacgttttt acgcagctgt
     5461 ttcacggtgg atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac
     5521 tgctggcgtg ggatcagatc tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa
     5581 ttatcacggt gggtgatcaa cgccagcgca tcaacacgtt caccgttgat taatacgtct
     5641 acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga cgcataacca
     5701 cgcgaggtag atttcaggcg atcgaagaag tcgagcacca cttccgccat cgggatctcg
     5761 tacgtaagcg ccacctgatt accgtggtaa accatattgg tctgcacgcc gcgtttttct
     5821 acgcacagcg taataacgtt gccgagatat gcctgcggca gcagcatgtg acactctgca
     5881 atcggctcgc gcagttcgta aatgttattt accgcaggca gcttggatgg gctgtcgacg
     5941 tagataactt cccttgacgt ggtttcaact tcatacacta cggtcggcgc agtggtgatc
     6001 agatccagat cgtattcacg ttccagacgc tcctggatga tctccatgtg cagcaggcca
     6061 aggaagccgc agcggaaacc aaagcccagc gcgctggagc tttccggctc atagaacagt
     6121 gaggcatcgt tcaggctgag tttacccagc gcgtcacgga aggcttcata gtcgtcggaa
     6181 cttaccggga acagaccggc gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc
     6241 ttttctgccg gattacgcgc cagcgttaag gtatcgccga ctggagcgcc gtggatatct
     6301 ttaatcgcac atacgagcca gcctacttcg ccacatttca gttcagtgcg gtcaacctgt
     6361 ttcggcgtga agatgcccag acggtcggcg ttataggtct gcccggtact catgactttc
     6421 actttgtcgc ccttacgcag ggtgccgttt ttaatacgga taagtgaaac aacgcccagg
     6481 tagttgtcga accatgagtc gataattagt gcctgcaacg ggccttccgg atcgccttcc
     6541 ggcggcggaa tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac gccggttttc
     6601 gctgaacagc gcaccgcgtc agtggcgtcg atgccgacga tatcttcaat ttcttccgcc
     6661 acgcgttcag gatcggctgc cggcaggtca atcttgttca gtaccggcac cacttcgaga
     6721 tccatttcca tggcggtgta gcagtttgcc agggtttgcg cttccacgcc ctgcccggcg
     6781 tcgaccacca gcaatgcacc ttcacaggca gccagcgaac gggaaacttc ataggagaag
     6841 tctacgtggc ccggggtgtc gataaagtta agctgatagg tttcgccgtc agacgctttg
     6901 tagtccagcg tcacgctttg cgctttgatg gtaatgccac gctcacgctc aagatccatg
     6961 gaatcgagaa cctgcgcctc catttcacgg tcagacaggc caccgcagat ctggataata
     7021 cggtcagaca gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa gttacgtata
     7081 ttcttcataa agtatgatta ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg
     7141 ctgttctgag cttaacgtct gtattaatag aaacgccgca ttctacacta caacattgag
     7201 gcgaggaaat gttcataccg tatggattgt ggtatctgga aacgtcctcg catttgttat
     7261 gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc
     7321 gcacaaggcc aggcggcaat gccacgctta agatgatcgg ttgccattct gcccgggcag
     7381 caaacttacg cgagtagcca cgcgcaatca ggaacccgcc aataccgccg aggaccgcac
     7441 cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg atgaggaata
     7501 atcccaccag cggcgacata taaaccagta atgcggagct aagcaggctg ccttcggcga
     7561 tccccaattc cactttttgc cccggcacta acggctcatc acagggtacg acaatggtat
     7621 gcgtggtttg cgggccaagt ttattcaaca cgcggctacc gcaaccggcg cgtgaagcgc
     7681 agctgctgca tgaggcttta acatcacaac tgaccagcgc ctgcccgttt tgccaggaga
     7741 cgacggtagc ccactctttg atcattgcgc tgccccgaac ttaatattct cggcaatgcg
     7801 tttcgccgtt tgcggcggca gttcaccgac aatggtgatt tcggcgttat cacgtacgct
     7861 tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg
     7921 gttaacgttt accgagaagc tgaataatcc gtcggaatag agacgtgatt cgataggcat
     7981 gttgtccatc gtcggtagcg gacgtcgact actggaaact tcgctaaaac cctgtggcaa
     8041 ccaggttggc gtccagctga atttagcttt ttcacctaca ggaacagaaa gcaacggcgg
     8101 caaatttgcc ttcgccagcg tctgcatact gctgctgata tcctgattga cgttaaaagc
     8161 aatcacgcga aattgttcca gcgtttcacc atcgcgatca agaagatcaa cccgcatcgg
     8221 taatttcgat tcggtgtcca tccacacgat gtagctgtag cgtgtaccat ctcgggcaac
     8281 cacgcgaatg acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg agataaagtc
     8341 gtagtaagga gaaaggcgtt tgaaatcggt atagatgagc gatggcagag aatcaacaat
     8401 gtaatcgcca ttaagcgtga acggttcaag tcccggttca aaatagctga tttcattgcc
     8461 gcgctgtacc acttcccggc gcgggccatc catttgcaac aattgtgcaa gaggacggtt
     8521 atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc tgtttattga tgctgatgaa
     8581 tgacagctcg taattcagtg actgactggc caggttcatc tgctgtaata acgccccgga
     8641 cgcgggagtg gccgaggcgt tagcagagaa taacaggcta cctgtcacta atgacatggc
     8701 aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag tttgaattcc tggcacctgt
     8761 acagcggctt gctgggtttg cgcctgctca aactgaagct gttcagagtg gagtcggcgt
     8821 tgcagttcgt aatcctgcaa cattgcatta atgcgacgac gctgctgctg ttgaccatta
     8881 tttgcggtcg cttcagaagg tactcccagg ctaaccgggc tggctttacc catcatcggc
     8941 agtgtattaa ataccggcgt ttcgggctgc tgggacgttt cagattgtcc attatagtgc
     9001 tggacgccaa cgataactgc aagcgatacg catgcggcta cgcccatttg ggtaagctgt
     9061 gccgcccacg gacgtacttt ctgccagaat ggcattttct gccattcatg cggcgcaggc
     9121 tgggcttccg ggatcaatgt cgccggttga cgtactggct cttcttcaat ggcggccatc
     9181 acgcgtgaag agatatcgaa atggagcacc tcgggagtat caccccgcat tgagtcacgg
     9241 attaagtgat agctttccca ggttttctgc atttctgggt tatgagccag ttcgttaagc
     9301 agctcactat ccagcgtttc gccatccatt aaagcggaaa gttgttcttt ctgcatgcct
     9361 aataccctta tccagtatcc cgctatcgtc aacgcctgat aagcggttga actttgttat
     9421 caatagcttc cctcgctcgg aagatacgtg aacgcaccgt acctaccgga caatccatga
     9481 tagcggctat ctcttcatag ctcaggccat ccagctcccg caaggttatt gccatgcgta
     9541 aatcttccgg gagggactca atagttcgga aaactatctg tctcagttct tctgacaaca
     9601 ttaagttctc agggttcgaa atttctttca acgcgccgcc actttcgaag ttttcagctt
     9661 caatggcatc cacatcactg gaaggtggac gacgcccctg agcaaccagg taatttttcg
     9721 ctgtatttac agcaatccga tacagccatg tataaaaagc gctatctccc cggaacgaat
     9781 ccagcgcacg ataggcttta ataaaagctt cttgtaccac atcgggaaca tcacccgacg
     9841 gcacatagcg ggaaaccaga ctcgccactt tatgctgata gcgcactacc agtaagttaa
     9901 aggctttctg atctcccttc tggacccgtt caaccaggac ctggtccgtt aactgctcgc
     9961 tcatccgagg taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac gccactccat
    10021 tagctgcaat ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg
    10081 catctgtttt tgtttgtcat gctgtagacg gatcattatc tatcattata agtctacaga
    10141 atctgaacat cgcattatct gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa
    10201 cggccttttt atttcaccac ctaatcctcc accagccagt aacttctctt tttctcgccg
    10261 ccctgcgtca gcgtgtttag tcactgtaac aaatattaaa atagcaggtg tttattcgca
    10321 caacatgatg ctatgctgac caaaccgtgt ttagtaaatt aaacaaagaa aatgaatact
    10381 ctccttgaac attcatgtga cgtgttgatt atcggtagcg gcgcagccgg gctttcactg
    10441 gcgctacgcc tggctgacca gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa
    10501 ggttcaacat tttatgccca gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt
    10561 gactcgcatg tggaagacac attgattgcc ggggctggta tttgcgatcg ccatgcagtt
    10621 gaatttgtcg ccagcaatgc acgatcctgt gtgcaatggc taatcgacca gggggtgtta
    10681 tttgataccc acattcagcc gaatggcgaa gaaagctacc atctgacccg tgaaggtgga
    10741 catagtcacc gtcgtattct tcatgccgcc gacgcaactg gtagagaagt agaaaccacg
    10801 ctggtgagca aggcgctgaa ccatccgaat attcgcgtgc tggagcgcag caacgcggtt
    10861 gatctgatta tttctgacaa aattggcctg ccgggcacgc gacgggttgt tggcgcgtgg
    10921 gtatggaacc gaaataaaga aaaggtggaa acctgccacg caaaagcggt ggtgctggca
    10981 accggcggtg cgtcgaaggt ttatcagtac accaccaatc cggatatttc ttctggcgat
    11041 ggcattgcta tggcgtggcg cgcaggctgc cgggttgcca atctcgaatt taatcagttc
    11101 caccctaccg cgctgtatca cccacaggca cgcaatttcc tgttaacgga agcactgcgc
    11161 ggcgaaggcg cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga ttttgatgtg
    11221 cgcggcgaac tggccccgcg cgatattgtc gcccgcgcca ttgaccatga aatgaaacgc
    11281 ctcggcgcag attgtatgtt ccttgatatc agccataagc ccgccgattt tattcgccag
    11341 cattttccga tgatttatga aaagctgctc gggctgggga ttgatctcac acaagaaccg
    11401 gtaccgattg tgcctgctgc acattatacc tgcggtggtg taatggttga tgatcatggg
    11461 cgtacggacg tcgagggctt gtatgccatt ggcgaggtga gttataccgg cttacacggc
    11521 gctaaccgca tggcctcgaa ttcattgctg gagtgtctgg tctatggctg gtcggcggcg
    11581 gaagatatca ccagacgtat gccttatgcc cacgacatca gtacgttacc gccgtgggat
    11641 gaaagccgcg ttgagaaccc tgacgaacgg gtaataattc agcataactg gcacgagcta
    11701 cgtctgttta tgtgggatta cgttggcatt gtgcgcacaa cgaagcgcct ggaacgcgcc
    11761 ctgcggcgga taaccatgct ccaacaagaa atagacgaat attacgccca tttccgcgtc
    11821 tcaaataatt tgctggagct gcgtaatctg gtacaggttg ccgagttgat tgttcgctgt
    11881 gcaatgatgc gtaaagagag tcgggggttg catttcacgc tggattatcc ggaactgctc
    11941 acccattccg gtccgtcgat cctttctccc ggcaatcatt acataaacag ataaaaagcc
    12001 tgggttagcg ccgtatacgc ttcggaatag ttctggtctg gcccacgaat gactaagcga
    12061 tcgctaaagc attctcccgc ctgcggggag aatgccagca gcacccgatg cggcagtcgc
    12121 gcttcgtttt ccgccacatc cgtccgcaaa cgtaaatgcc agcccatact taatgccagc
    12181 tccgtaaaac tattaccaat ctgctctggc agcaccacgc agaaaaatcc ctcttcggta
    12241 atgcactccg ccgcgcaagt aagtaaagtc tggtgatcaa gtgaggtggt gtagcgcgcc
    12301 tgttcccgct gcggcgtcgc gcactccact ccttgctcat aatacggcgg gttgctgatg
    12361 attaaatcaa agcgcgcggt ctgctgtgtt acccactgct gaatatccgc cgtatggacg
    12421 ttaattctct ctaaccacgg ggactgggcg acattttcct gcgcctgagt ggcagcttca
    12481 ctttccagct caacggcatc aattatcacg ctgtcactgg ttcgctgcgc cagcatcagt
    12541 gccagcaagc cactacccgc accgatatca aggcaacgtt ttaccccagc caccggtgcc
    12601 catgcgccca gcaatatgcc atccgttccc actttcatcg cacagcgatc gtgagcaaca
    12661 aaaaactgtt taaaagtaaa tccattacga cgaagcacgg atgtagactg tgacatgaaa
    12721 ataaaacctt gcaggaaaaa cggcgatagc accgggtgag aacaataccc gagaagcgat
    12781 atccatacaa acagatgaag attgcagccg taacgtctat aatcagcgcc ccacacagag
    12841 gtagaacatg actgtaacga ctttttccga acttgaactc gacgaaagcc tgctggaagc
    12901 cctccaggat aaaggtttca ctcgcccgac cgccattcag gctgccgcca ttccgcctgc
    12961 gctcgatggc cgtgatgtac tcggttctgc gccgacaggc accggtaaaa cggcggcgta
    13021 tctgctgcca gcgttgcagc acctgctcga tttcccgcgt aagaaatccg gtccgccgcg
    13081 tattttgatc ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc atgcccgcga
    13141 actggcgaaa catacgcatc tggatatcgc caccatcacc ggcggcgtag cctatatgaa
    13201 ccacgcggaa gtgttcagcg aaaatcagga catcgtggtc gccacgaccg gacgtctgct
    13261 gcaatacata aaagaagaga acttcgattg ccgcgcggtt gaaacgctga tcctcgacga
    13321 agcagaccgt atgctggata tgggcttcgc tcaggatatc gaacatattg ctggcgaaac
    13381 gcgctggcgt aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg cgattcagga
    13441 ctttgccgag cgtctgctgg aagatccggt ggaggtttct gccaatccgt ccacccgtga
    13501 gcgcaaaaaa attcatcagt ggtattaccg cgccgatgac ctggaacata aaaccgcatt
    13561 gctggtgcat ctgttaaaac agccggaagc gacccgctca attgtgtttg tgcgtaagcg
    13621 tgagcgtgtg catgagctgg caaactggct gcgcgaagcg ggcatcaaca actgctatct
    13681 cgaaggtgag atggtacagg gcaagcgtaa cgaagcgatc aaacgtttga ccgaaggtcg
    13741 cgtaaacgta ctggttgcaa ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag
    13801 ccacgtcttt aacttcgata tgccgcgcag tggcgatact tatttgcacc gtatcggacg
    13861 taccgcgcgc gccggtcgta aaggcaccgc aatttcgctg gtagaagccc atgaccatct
    13921 gctgctgggc aaagttggtc gctatattga agagccaatt aaagctcgcg taattgatga
    13981 gttacgcccg aaaacgcgtg caccaagcga gaagcagacc ggcaagccat cgaagaaagt
    14041 actggcaaaa cgcgccgaga agaaaaaagc taaagagaaa gagaagccgc gggtgaaaaa
    14101 acgccatcgc gataccaaaa atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc
    14161 accgcaaacg acagaagagt aatctcaatg ccgggtttaa gcccggcatt atttttatcc
    14221 cggcaaaaaa caatcttcaa cacactgaat aaaagtgtgc atcgccgggc taaccgcttt
    14281 tccggcatgg tgagcgcaca ttgccgtaat ggtctgcgag tgttcgccaa aaggcaattc
    14341 aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt aactgacacc
    14401 gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa tggtgttttc
    14461 caccgtgatc cgccgctggc gcagcgtgct ctcaaatatc tgccggaata cacattgcgg
    14521 ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat cgacatctgc
    14581 aatttgcggt gaagctacca gcaccagcga ttgctcaccc aattctcgtc ggttcagggc
    14641 atcatcattc cctacgcgat aaaaaacccc gacatcggcc tcatcattca atagcgcatc
    14701 acggatcacg taacagttca gagactgcaa cgataaacgc acttttgggg cgcgttgccg
    14761 aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg aaacaacgcg
    14821 tagttccccg tccggatccg actccttttt ggcagcctca cgaagggtat ccatcactct
    14881 ggtgagttca taaatgtgcg gcagcagctt tttaccttcg cgagtgaggc acatccgtcg
    14941 gccaattttc tcaaataact gtaccgagaa ctcctgctca agctgctgaa tatgaaaagt
    15001 caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac cctcttccac
    15061 cacggtttta agcgtaataa aacggcgcag atccataacc ccaaacctat cgaaaatatc
    15121 gaatttagaa tgtaaaaaca ttcatttttt taaatattct gtgtcgggta ctgtctacca
    15181 aaacagagga gataacaagt gacaccgacc cttttaagtg ctttttggac ttacaccctg
    15241 attaccgcca tgacgccagg accgaacaat attctcgccc ttagctcagc cacaacgcac
    15301 ggatttcatc agagtacccg cgtgctggca gggatgagtc tgggattttt aatcgtcatg
    15361 ttgctgtgtg cgggcatttc gttttcactg gctgttatcg acccggcagc tgtgcatctg
    15421 ttgagttggg cgggggcggc atatattgtc tggctggcgt ggaaaatcgc taccagtcca
    15481 acaaaggaag acgggcttca gacaaaacca atcagttttt gggccagctt tgctttgcag
    15541 tttgtgaacg tcaaaatcat tttgtacggt gttacggcac tgtcgacatt tgttctgccg
    15601 caaacacagg cgttaagctg gatagttggc gtcagcgttt tgctggcaat gattgggacg
    15661 tttggtaatg tgtgctgggc gctggcgggg catctgtttc agcgattgtt tcgccagtat
    15721 ggtcgccagt taaatatcgt gctggcgctg ctgctgatct attgcgcggt acgcattttc
    15781 tattaatgaa aaaaagcgga agaggtcgcc ctcttccgct tagtaacatg ctacttaagc
    15841 cttacaggct ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag
    15901 agttaaagcg aactgcataa ccggatacac ggatggtcag ctgcggatat ttttccggat
    15961 gcttaactgc atcttccaga gtttcacgac gcagaacgtt aacgttcagg tgctgaccac
    16021 cttcaacgcg aacttctggt ttcacttcta ctggaacttc acggtactca atgtcaccca
    16081 gtttgcttac tgcaaccact tcgtcttctg cataacctgc tttcgcaacg atgcaacgcg
    16141 cttcgccttt ttcgctgtcc agcagccaga aagagttcag cagatcgtcg ttagcggctt
    16201 tagtaatctg gatacctgta atcatgtgat gcctccccgg caaaattatt tgatttgttc
    16261 agcctgtcgc ggccaattgg taaaaccatt gttgcttgag tgtatatata ctcctcaaac
    16321 acccttgaat ctttgattta aatcaataaa aaccacacat caagtgtggt cgcaaataga
    16381 ttttattgtt ttacatcaac ttatgcgggt gtgaaatttt accaatttac atttttttgc
    16441 actcgtttaa gtctaaaaaa tgagcatgat tttgttctgt agaaagaagc agttaagcta
    16501 ggcggattga agattcgcag gagagcgaga tggctaacga attaacctgg catgacgtgc
    16561 tggctgaaga gaagcagcaa ccctattttc ttaataccct tcagaccgtc gccagcgagc
    16621 ggcagtccgg cgtcactatc tatccaccac aaaaagatgt ctttaacgcg ttccgcttta
    16681 ccgagttggg tgacgttaaa gtggtgattc tcggccagga tccttatcac ggaccgggac
    16741 aggcgcatgg gctggcattt tccgttcgcc ccggaatcgc cactcctccg tcactgctga
    16801 atatgtataa agagctggaa aatactattc cgggcttcac ccgccctaat catggttatc
    16861 ttgaaagctg ggcgcgtcag ggcgttctgc tactcaatac tgtgttgacg gtacgcgcag
    16921 gtcaggcgca ttcccacgcc agcctcggct gggaaacctt caccgataaa gtgatcagcc
    16981 tgattaacca gcatcgcgaa ggcgtggtgt ttttgttgtg gggatcgcat gcgcaaaaga
    17041 aaggggcgat tatagataag caacgccatc atgtactgaa agcaccgcat ccgtcgccgc
    17101 tttcggctca tcgtggattc tttggctgca accattttgt gctggcaaat cagtggctgg
    17161 aacaacatgg cgagacgccg attgactgga tgccagtatt accggcagag agtgagtaaa
    17221 tttgcgggaa aatgccggat ggcagagttg ccacccggct gatttatcag gctttattct
    17281 gacgccacca ttcgccaaga agaacgccgg ttgcgacaga gatattcagc ccggcaacgt
    17341 tgccagtacc atcaatcttc acgcgcagat cgttcggatc gcgtgcggca tccggtaacc
    17401 cttcatattc ctgacccagc accagtacca ttttcgctgg cagactggtt ttgaacagcg
    17461 gtttaccctg ctcgctggaa gttgtcacta cggtgtaacc cgcctgacgg aaatcatcca
    17521 gcacgttaac aatgttgtcg ccagtaatcg gctgaacgtg ctctgcgccg ccttctgcgg
    17581 tacggatagc cgcccccgac tccagcagtg ccgcatcctg caccacaaca cctttcacgc
    17641 caaagtgcgc gcagctgcgc atcatgccac ccaggttatg cgggttagat tcgttttcca
    17701 gtgccagaac acaatcctgc gcgcctgcct ggcttaccca ctgctgcacg gttgtgccat
    17761 tacgcttttt gatcaggaag caaacgccac cgtgatgttc cgtgcctgac gcttttgtca
    17821 attccgcttc atctaccaca tggtacgctt tgcggtttgc tgccatccag cgcaaggctt
    17881 ctttaaaacg cggcgttaca ctctggataa accaggcgcg aacaatcgct tccggacggc
    17941 tctggaacag tgcctgacag gcgttttcgc cgtagacgcg ggtttcttcc gcacgctgac
    18001 gacgcaacac ttccggatca ataaaacttt taccactgat gccaccgtga tcggcctttt
    18061 ccggcgtctc atcacccggc gcgcgggaaa ccgtgcgcca cggcgaatct tcccacttgc
    18121 ggtcacgggg ctgactctgt ttgtcatcgc gggcggggcg acggccaccg tcagcacgag
    18181 attttcctgg acgcccgccc cctttcccgg tacgcgggtt gtgggtacgt ttatcagaat
    18241 catcatcact gcggacatac atcactttga ccttgccgct tttacctttc atttcatcgt
    18301 tcatgctttt ctccaccagc gctgcgcgaa gcgcgcagat tacccgaagt ccgcgcggtt
    18361 cgccatgatt tcgtaccaaa gcctgcgact atcataccta ttgaataaaa cagattgttg
    18421 tctggaacaa tgtccccgat aatatgtaac atattagaaa cataccggcg ttgttgccga
    18481 taagtctcct tactcatccc gaggttagtt atgaataccg tttgtaccca ttgtcaggcc
    18541 atcaatcgca ttcccgacga tcggatcgaa gatgcggcaa aatgcggacg ctgcggtcac
    18601 gacttgtttg acggagaggt gattaatgcg accggtgaaa cgctcgacaa attgctgaaa
    18661 gatgatctac cagtggtgat cgacttctgg gcaccgtggt gcggcccctg ccgtaatttc
    18721 gcaccaattt ttgaagatgt cgcgcaagag cgtagcggta aagtgcgctt tgtgaaagtg
    18781 aataccgaag ctgaacgtga attgagcagt cgctttggaa ttcgtagtat accgacgatc
    18841 atgattttca aaaacggtca ggttgtcgac atgcttaatg gcgcagtacc gaaagcgccg
    18901 ttcgatagct ggctgaacga atctctttaa tcttaccggg gcgcatcttg tgccccgttt
    18961 tctcctctgc gacaatggcg ttttttcgac gctctcttat gaccgaaaac gctgttctcc
    19021 agttacgcgc cgagcgtatt gcgcgcgcaa cacgtccttt tcttgcccgc ggtaatcgcg
    19081 ttcgtcgctg ccaacgctgt cttttgccag agaaattatg tctctgttcg acaattaccc
    19141 cagcacaagc aaaaagtcgc ttctgtttgc tgatgttcga caccgagcca atgaagccca
    19201 gtaataccgg gcgtctgatt gctgatattt tgcctgatac cgttgcgttt caatggtcgc
    19261 gtaccgaacc ctcgcaggat ttgctggatc tggtacaaaa cccggattat cagccaatgg
    19321 tggtctttcc cgcctcgtat gctgatgagc aacgggaagt gatcttcaca ccacctgccg
    19381 gtaagccacc gctgtttatc atgctcgatg gtacctggcc ggaagctcgc aagatgtttc
    19441 gtaaaagtcc gtatctggat aatcttcccg tcatttccgt cgatctttcc cggctttctg
    19501 cctatcgcct gcgtgaagcc caggctgaag gccaatattg tactgccgag gtagccatcg
    19561 cactgttaga tatggccggc gataccgggg cggcagcagg gttaggcgag cacttcaccc
    19621 gctttaaaac gcgctatctg gcaggaaaaa cgcaacatct gggtagcatc acagcagaac
    19681 agttagaaag cgtttaaaat cattcggtca cttctgcggg agaccggtat gagtcagcga
    19741 ggactggaag cactactgcg accaaaatcg atagcggtaa ttggcgcgtc gatgaaaccc
    19801 aatcgcgcag gttacctgat gatgcgtaac ctgctggcgg gaggctttaa cggaccggta
    19861 ctcccggtga cgccagcctg gaaagcggtg ttgggtgtgt tggcctggcc ggatattgcc
    19921 agcttgccct ttacacccga ccttgcggtt ttatgtacca atgccagccg taatcttgct
    19981 cttctggaag agctcggcga gaaaggctgt aaaacctgca ttattctttc cgccccggca
    20041 tcgcaacacg aagatctccg cgcctgcgcc ctgcgccata acatgcgcct gcttggacca
    20101 aacagtctgg gtttactggc tccctggcaa ggtctgaatg ccagcttttc gcctgtgccg
    20161 attaaacgcg gcaagctggc gtttatttca cagtcggctg ccgtctccaa caccatcctc
    20221 gactgggcgc aacagcgtga gatgggcttt tcctacttta ttgcgctcgg cgacagccta
    20281 gatatcgacg ttgatgaatt gcttgactat ctggcacgcg acagtaaaac cagcgccatc
    20341 ctgctctatc tcgaacagtt aagcgacgcg cgacgctttg tttcggcggc ccgtagtgcc
    20401 tcgcgtaata aaccgattct ggtgattaaa agcggacgta gcccggcagc acagcgactg
    20461 ctcaacacga cggcaggaat ggatccggcg tgggatgcgg cgattcagcg tgccggtttg
    20521 ttgcgggtgc aggataccca cgagctgttt tcggcggtgg aaaccctcag ccatatgcgc
    20581 ccgctgcgtg gcgaccggtt gatgattatc agcaacggtg ctgcgcctgc cgcgctggcg
    20641 ctggatgcct tatggtcacg caatggcaag ctggcaacgc taagcgaaga aacctgccag
    20701 aaactgcgcg atgcactgcc aggacatgtg gcaatctcta acccgctcga tctgcgcgat
    20761 gacgccagca gcgagcatta tgtcaaaacg ctggacattc tgctccacag ccaggatttt
    20821 gatgcgctga tggttattca ttcgcccagc gccgctgctc ccgcaacaga aatcgcgcaa
    20881 gtattaattg aagcggtaaa gcatcatccc cgcagcaagt atgtttctct gctgacgaac
    20941 tggtgcggcg agcactcctc gcaagaggca cgacgtttat tcagcgaagc cgggctgccg
    21001 acctaccgta ccccggaagg aaccatcact gcttttatgc atatggtgga gtaccggcgt
    21061 aatcagaagc aactacgcga aacgccggcg ttgcccagca atctgacttc caataccgca
    21121 aaagcgcatc ttctgttgca acaggcgatt gccgaagggg ctacgtcgct cgatacccat
    21181 gaagttcagc ccatcctgca atcgtatggg atgaacacgc tccctacctg gattgccagc
    21241 gatagcaccg aagcggtgca tattgccgaa cagattggtt atccggtggc gctgaaattg
    21301 cgttcgccgg atattccaca taaatcggaa gttcagggcg tcatgctgta cctgcgtaca
    21361 gccaatgaag tccagcaagc ggcaaacgct attttcgatc gcgtaaaaat gacctggccg
    21421 caggcgcggg tccacggcct gttggtgcaa agtatggcta accgtgctgg cgctcaggag
    21481 ttgcgggttg tggttgagca cgatccggtt tttgggccgt tgatcatgct gggtgaaggc
    21541 ggtgtggagt ggcgtcctga agatcaagcc gtcgtcgcac tgccgccgct gaatatgaac
    21601 ctggcccgct atctggttat tcaggggatc aaaagtaaaa agattcgtgc gcgcagtgcg
    21661 ctacgcccat tggatgttgc aggcttgagc cagcttctgg tgcaggtttc caacttgatt
    21721 gtcgattgcc cggaaattca gcgtctggat attcatcctt tgctggcttc tggcagtgaa
    21781 tttaccgcgc tggatgtcac tctggatatc gcgccgtttg aaggcgataa cgagagtcgg
    21841 ctggcagtgc gcccttatcc gcatcagctg gaagaatggg tagaattgaa aaacggtgaa
    21901 cgctgcttgt tccgcccgat tttgccagaa gatgagccac aacttcagca attcatttcg
    21961 cgggtcacca aagaagatct ttattaccgc tactttagcg agatcaacga atttacccat
    22021 gaagatttag ccaacatgac gcagatcgac tacgatcggg aaatggcgtt tgtagcggta
    22081 cgacgaattg atcaaacgga agagatcctc ggcgtcacgc gtgcgatctc cgaccctgat
    22141 aacatcgatg ccgaatttgc cgtgctggtt cgctcggatc tcaaagggtt aggcttgggt
    22201 cgacgcttaa tggaaaagtt gattacctat acgcgagatc acggactaca acgtctgaat
    22261 ggtattacga tgccaaacaa tcgtggcatg gtggcgctgg cccgcaagct cgggtttaac
    22321 gttgatatcc agctcgaaga ggggatcgtt gggcttacgc taaatcttgc ccaacgcgag
    22381 gaatcatgag taaggtactg gaaatgttga ccacttaatc gggactggtg ttattattgc
    22441 acgcttatgt cgtctgcatt gcacagagga cccttcaatg aacagagaag aaatgcactg
    22501 tgatgttgtc aaaatttaag cgtaataaac atcaacaaca ccttgcccaa ctacccaaga
    22561 tttctcaatc agttgatgat gtcgatttct tttacgctcc cgccgacttc cgggagacgc
    22621 tgctggaaaa aatagccagc gcgaagcagc gcatttgcat tgtcgccctg tatctcgaac
    22681 aggatgacgg tggcaaaggc attctgaacg cgttgtatga agctaaaagg cagcgtccgg
    22741 aactggatgt gcgggtactg gtcgactggc atcgtgcaca acgtggacgc attggcgctg
    22801 cggcatctaa cactaacgct gactggtact gtcgcatggc gcaggaaaat ccgggcgtag
    22861 atgttccggt ttatggcgtt ccaatcaata cccgtgaagc ccttggtgtt ctgcacttta
    22921 aaggctttat catcgacgat agcgtacttt atagcggtgc cagcctgaac gatgtttacc
    22981 tgcatcagca cgataaatat cgctacgacc gttatcatct tatccgtaac cgtaagatgt
    23041 cagacattat gtttgaatgg gttacacaga atattatgaa tggccgcggc gttaatcgtc
    23101 tggatgatgt taatcggcca aaaagcccgg aaatcaagaa cgatattcgt ctgttccgcc
    23161 aggagctgcg tgatgccgct tatcatttcc agggcgatgc cgacaacgat cagctttccg
    23221 taacgccgct agtggggctg gggaaatcga gtctgttgaa caagaccatt ttccatctta
    23281 tgccttgtgc cgagcagaaa ctaaccatct gtacgccata cttcaacctg ccagcaatcc
    23341 ttgtgcgcaa tattatccag ttgctgcgcg aagggaaaaa ggtcgaaatt attgttggtg
    23401 ataaaaccgc gaacgacttc tacattccgg aagatgaacc tttcaagata attggcgcat
    23461 tgccttatct ctatgagatc aatctgcgtc gtttcctgag ccgtttgcag tattacgtca
    23521 atactgacca gctagtggtt cggttatgga aagatgacga caacacctat cacctaaaag
    23581 ggatgtgggt tgatgataag tggatgttga tcaccggtaa taacctgaac ccgcgcgcct
    23641 ggcgtctgga tctggaaaac gccattttga tccacgatcc acaactggag cttgcgccgc
    23701 agcgagagaa agaactggag ctgatccgcg agcataccac catcgttaag cactatcgcg
    23761 atctgcaaag tattgccgat tatccggtga aggttcgtaa actcatccgc cgtttgcgcc
    23821 gtatccgcat cgaccgatta attagccgca tcctgtaatc acaaccccgt cctgtacggg
    23881 gtttgttttt tggaggccac gttttgcgta ttctttttgt ctgttcgcta ttgttacttt
    23941 ctggatgcag ccatatggct aacgatagct ggagcgggca ggataaagct caacacttta
    24001 tcgcctcggc gatgctttcc gccgccggaa atgaatattc acagcatcag gggatgagcc
    24061 gggatcgcag tgccatgttt ggtttgatgt tctctgtcag tttgggggcg tcaaaagagt
    24121 tttgggatag ccgccccgaa gggagcggct ggagctggaa ggatttggcc tgggatgtcg
    24181 ccggtgcaag caccggctat accgtctggc aactgacccg tcactaaaga cgcatcccct
    24241 tccctttgcg atgtagcatc aaagaaacca gaaacgccac cacggccatc aaggtcacat
    24301 accagaagaa ggctgtttcc attcctattg atttcagcga caacgctacg tactccgccg
    24361 aaccaccaaa tatagcatta gcgaccgcat atgacagacc aacgcctaat gcgcgaacct
    24421 gtgccgggaa catctcagcc ttcagtattc cactgattga tgtataaaaa ctcactatca
    24481 gcagggcaca catcaccaga ccaaaagcgg cataaggcga ggaaacgttt tgcaatgctg
    24541 agagaatagg aacggtaaaa atggctgcca gcgaaccgaa acataacatt gaggtacggc
    24601 gaccaatctt atccgacagc gcgccaatga gtggttgaat aagcatgaat acaaacaatg
    24661 cggcagtcat aatgccactc gccacgttgg catgcattcc cgcagtattt accagatact
    24721 tctgcatata agtggtgaag gtatagaaac aaagggagcc cgcagcggta aaaccgagaa
    24781 ccatgatgaa tgcacggcga ttgcgccata atcctttcag agatccagct tcttttaaag
    24841 cgcgcgtttc ttgttgcgaa gtttcatcta actgacgacg taaccacaac gccacaacag
    24901 ctaacacagc tcctaacgcg aaaggaatac gccatcccca ctctctgagt gcagagtctt
    24961 ccatggtgtg ttgtaaaacc acgacaacca gtagggctag cagttgtccg ccgatcaacg
    25021 tcacatactg aaatgatgcg taaaaacctt tgcgcccttc aacggcaact tcactcatat
    25081 aggtggcgct ggtgccatat tctccgccaa cggataatcc ctgaaataaa cgcgcgagaa
    25141 gcaataatgc cggagcccac gtaccgattg tttcataacc aggcaaacag gcgattacca
    25201 gtgagccgaa acacatcata cacaccgata gcagcatcga ttttttgcga ccatgtttat
    25261 cggctatgcg gccaaatagc caaccgccta ttgggcgcat caggaatccc gcagcaaaaa
    25321 cacctgctgt ttgtagtagt tgagtcgtcg tgttcccgga agggaagaag atgtgggcaa
    25381 agtagagtga acagaacgag tagacataga aatcgaacca ctcgaccaga ttacctgaag
    25441 aggcccccac aatcgcccaa atgcggcgac gagtatcact acttgtcagt ttgctgtctg
    25501 ccgttacagt actttcagcc atgccattat gtctcctgcc gtaatccgat gcttttgtcg
    25561 gtcgcttttg tttatttctt ttggcaaaga aaatgttata catttgttac gtatcattat
    25621 gcaaccttaa ccatgaattt agttagcagg aaaatggtta ttgaggagct taaggataaa
    25681 tttctggtaa ggaggacacg tatggaagta ggcaagttgg ggaaaccgta tccgttgctg
    25741 aatctggcat atgtgggagt ataagacgcg cagcgtcgca tcaggcattt ttttcaccaa
    25801 cgcaaaaagg ccatcctgac ggatggcctt tcgcttaatt tgatgcctgg cagttcccta
    25861 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
    25921 gcatggggtc aggtgggacc accgcgcta
//
LOCUS       DAZGXS010000062        25854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGXS010000062 DAZGXS010000000
VERSION     DAZGXS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..370)
                     /locus_tag="ACHKG5_002646"
     CDS             complement(<1..370)
                     /locus_tag="ACHKG5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868727.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     SGNENLVNISAEKIDEIKNSKNFFSNIVNIIILLIKHPDKILPILHGCPVESIEVIKN
                     AKRTGQWSYEMTDKNGQSIEV"
     gene            complement(558..2375)
                     /gene="malZ"
                     /locus_tag="ACHKG5_002647"
     CDS             complement(558..2375)
                     /gene="malZ"
                     /locus_tag="ACHKG5_002647"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0868728.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2531..3904)
                     /gene="proY"
                     /locus_tag="ACHKG5_002648"
     CDS             complement(2531..3904)
                     /gene="proY"
                     /locus_tag="ACHKG5_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0868729.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(3980..5299)
                     /gene="brnQ"
                     /locus_tag="ACHKG5_002649"
     CDS             complement(3980..5299)
                     /gene="brnQ"
                     /locus_tag="ACHKG5_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0868730.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5706..7001)
                     /gene="phoR"
                     /locus_tag="ACHKG5_002650"
     CDS             complement(5706..7001)
                     /gene="phoR"
                     /locus_tag="ACHKG5_002650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0868731.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(7059..7748)
                     /gene="phoB"
                     /locus_tag="ACHKG5_002651"
     CDS             complement(7059..7748)
                     /gene="phoB"
                     /locus_tag="ACHKG5_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0868732.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            7938..9140
                     /gene="sbcD"
                     /locus_tag="ACHKG5_002652"
     CDS             7938..9140
                     /gene="sbcD"
                     /locus_tag="ACHKG5_002652"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0868733.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            9137..12280
                     /gene="sbcC"
                     /locus_tag="ACHKG5_002653"
     CDS             9137..12280
                     /gene="sbcC"
                     /locus_tag="ACHKG5_002653"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0868734.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12406..13590
                     /gene="araJ"
                     /locus_tag="ACHKG5_002654"
     CDS             12406..13590
                     /gene="araJ"
                     /locus_tag="ACHKG5_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0868735.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSKTAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13733..14641)
                     /gene="mak"
                     /locus_tag="ACHKG5_002655"
     CDS             complement(13733..14641)
                     /gene="mak"
                     /locus_tag="ACHKG5_002655"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP0868736.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14766..15677
                     /gene="rdgC"
                     /locus_tag="ACHKG5_002656"
     CDS             14766..15677
                     /gene="rdgC"
                     /locus_tag="ACHKG5_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0868737.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15762..16211)
                     /locus_tag="ACHKG5_002657"
     CDS             complement(15762..16211)
                     /locus_tag="ACHKG5_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868738.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(16208..18487)
                     /locus_tag="ACHKG5_002658"
     CDS             complement(16208..18487)
                     /locus_tag="ACHKG5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFP0868739.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(18631..18915)
                     /gene="ppnP"
                     /locus_tag="ACHKG5_002659"
     CDS             complement(18631..18915)
                     /gene="ppnP"
                     /locus_tag="ACHKG5_002659"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0868740.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(18987..19664)
                     /gene="aroM"
                     /locus_tag="ACHKG5_002660"
     CDS             complement(18987..19664)
                     /gene="aroM"
                     /locus_tag="ACHKG5_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0868741.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHLVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(19922..20113)
                     /gene="yaiA"
                     /locus_tag="ACHKG5_002661"
     CDS             complement(19922..20113)
                     /gene="yaiA"
                     /locus_tag="ACHKG5_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0868742.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(20163..20687)
                     /gene="aroL"
                     /locus_tag="ACHKG5_002662"
     CDS             complement(20163..20687)
                     /gene="aroL"
                     /locus_tag="ACHKG5_002662"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0868743.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20870..21328)
                     /gene="yaiI"
                     /locus_tag="ACHKG5_002663"
     CDS             complement(20870..21328)
                     /gene="yaiI"
                     /locus_tag="ACHKG5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0868744.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21448..22257
                     /gene="proC"
                     /locus_tag="ACHKG5_002664"
     CDS             21448..22257
                     /gene="proC"
                     /locus_tag="ACHKG5_002664"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0868745.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(22274..23389)
                     /gene="adrA"
                     /locus_tag="ACHKG5_002665"
                     /gene_synonym="dgcC"
     CDS             complement(22274..23389)
                     /gene="adrA"
                     /locus_tag="ACHKG5_002665"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0868746.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23491..23811)
                     /gene="psiF"
                     /locus_tag="ACHKG5_002666"
     CDS             complement(23491..23811)
                     /gene="psiF"
                     /locus_tag="ACHKG5_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0868747.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(23930..25345)
                     /gene="phoA"
                     /locus_tag="ACHKG5_002667"
     CDS             complement(23930..25345)
                     /gene="phoA"
                     /locus_tag="ACHKG5_002667"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0868748.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(25446..25706)
                     /gene="iraP"
                     /locus_tag="ACHKG5_002668"
     CDS             complement(25446..25706)
                     /gene="iraP"
                     /locus_tag="ACHKG5_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0868749.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         6524 a         6823 c         6277 g         6230 t
ORIGIN      
        1 acacttcaat actctgcccg ttcttatcag tcatctcata tgaccattgt cccgttcgtt
       61 tcgcattctt aatgacttcg atcgactcta caggacaacc atgaagaatg ggcagaattt
      121 tatcaggatg cttaatgagt aaaattatta tatttacaat gtttgaaaag aaatttttgc
      181 tattttttat ttcatcgatt ttttccgctg aaatgtttac taagttttca tttccggaaa
      241 tgaaaagcga tttgccatag gtgctttttt taaattgttc ttctgtttta gaaaagacat
      301 cctctttaaa aggtggcaac tcagtaccag taataaacgg tttcaataaa ttcgaatttg
      361 aaattgtcat atctttgctc cactacaggg ttatcctgga gcatgttgta cataacatca
      421 tgggcaaaca ataccaaaaa tcgctcatat cgatcgggac aaatatttaa caaattcagc
      481 cctgacattg agcagctttt ttgcgctgcg tagtgtcagg gctggtgact aacgacgtaa
      541 caaataaaac agatgcttta gttcatccac accgtagccg aaatggcagg caaagccaga
      601 atcccgtctg tcagttgccc atggccttct ttgcgttgcc attgcacgac attaagaaac
      661 ggtgacgcgg gtagcaccac ttcacaggct tcgccacggt taattgccac cagtacacgc
      721 tgctgattca gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag
      781 ccgccacgac gcagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacaacgcg
      841 aataacgcca tatcctgttt ttccacctgc caggggaacg gcttacggca aaacggatca
      901 tttttgccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
      961 aacagccaga ccactgccag gggcaggcgc gcaacctccc gaccgagcag cgttttaaat
     1021 cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgctgttg atgagaaagc
     1081 cctgcgcggt aattatccat ccaggccata caggtttgag cgtcaatttg ctgcggatcg
     1141 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc gcgatagttc
     1201 atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgctctcca
     1261 actatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccagc aacgtgctgc
     1321 aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag ccgccagccg
     1381 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
     1441 tcattcacca gactttccga ctgataatcc agcttcggta agctggcata gccaagccag
     1501 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcggggttg
     1561 tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg
     1621 tggttaaaca cgccatccag caccagccgc attcccagct gctgcgtatt gtggcgtaag
     1681 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
     1741 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc
     1801 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
     1861 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
     1921 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
     1981 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca
     2041 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
     2101 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
     2161 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
     2221 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
     2281 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
     2341 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
     2401 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
     2461 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
     2521 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc
     2581 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
     2641 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
     2701 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
     2761 aggcaatttg cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg
     2821 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
     2881 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
     2941 aaattttcgg cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
     3001 catcactgtt aattgccgac agcgaagcag tcagcacaac aaagttaaga atgctggcgg
     3061 caaaggtaat gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct
     3121 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
     3181 gcatcggcac ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg
     3241 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
     3301 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
     3361 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
     3421 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aataccttca
     3481 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
     3541 gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
     3601 caataaggat ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt
     3661 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
     3721 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
     3781 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
     3841 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt
     3901 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
     3961 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
     4021 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
     4081 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
     4141 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
     4201 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
     4261 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca
     4321 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
     4381 caaagaattc tgcacaagca caggtcaggc caactgccgt taccaggcag gcgatgaaga
     4441 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
     4501 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga
     4561 acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat
     4621 aacgggtcag taggcgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
     4681 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttgacg aagccgttag
     4741 aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
     4801 tcgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
     4861 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
     4921 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
     4981 aagaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
     5041 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
     5101 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
     5161 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
     5221 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
     5281 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
     5341 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
     5401 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
     5461 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
     5521 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
     5581 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
     5641 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
     5701 gcggattaat cgctgttttt gacaattaaa cgttccggga taacaaaact gaaacgtgtt
     5761 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
     5821 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
     5881 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
     5941 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
     6001 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatca cactgcgtag ctgatcttcg
     6061 ttgccagaca ccttgaggcc gttatctatc tcgaaggtaa atgtctgttt tttctgactc
     6121 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
     6181 agcaattgcg tcggtgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
     6241 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
     6301 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
     6361 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
     6421 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
     6481 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcaaatat
     6541 tgcgtaaact ccgggtaacg cagcaggtta aggatgttct gcccgttatc ttccggccag
     6601 cgcaaaccaa gaatctgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
     6661 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
     6721 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
     6781 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
     6841 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
     6901 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
     6961 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
     7021 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
     7081 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
     7141 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
     7201 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
     7261 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
     7321 gatgtcgggt cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa
     7381 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg
     7441 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
     7501 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
     7561 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
     7621 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
     7681 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
     7741 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
     7801 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
     7861 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt
     7921 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
     7981 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
     8041 cacaaaccca tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc
     8101 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
     8161 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
     8221 atatcatggc gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
     8281 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc
     8341 gtgacattat taccagccag gcggggctta acggtattga aaaacagcag catttactgg
     8401 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
     8461 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg
     8521 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
     8581 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
     8641 ttcgctattg cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg
     8701 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
     8761 taacgcaacc catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
     8821 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
     8881 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgttgaag
     8941 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
     9001 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac
     9061 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg
     9121 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
     9181 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
     9241 taccggcccg accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
     9301 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
     9361 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
     9421 ctggagccag aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga
     9481 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
     9541 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
     9601 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
     9661 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
     9721 ggcccgcaca gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt tgctcacgcc
     9781 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
     9841 acttaccgcg cagcagcaag aacaacaatc actaaactgg ttaacgcgtc tggacgaatt
     9901 gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa
     9961 agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc ttcgtccgca
    10021 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat actcgccagc agattgaaga
    10081 agtaaatact cgcttacaga acacaatggc gcttcgcgcg agcattcgcc accacgcggc
    10141 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    10201 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    10261 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    10321 acttaatgcg cttgcggcga tcacgttgat gttaaccgcc gatgaagttg ctaccgccct
    10381 ggcgcaacat gctgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    10441 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    10501 acagacgcaa cgtaatgccg cacttaacga aatgcgccag cgttataaag aaaagacgca
    10561 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    10621 tcaacgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccatcc
    10681 ggcggtcgag gcgtatcagg cgctggagcc tggcgttaat cagtctcgat tactggcgct
    10741 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    10801 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    10861 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    10921 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    10981 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    11041 tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg gttatgcact
    11101 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    11161 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    11221 gccgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    11281 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    11341 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga
    11401 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    11461 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    11521 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    11581 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    11641 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    11701 tgcagataac cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca
    11761 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    11821 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    11881 ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    11941 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    12001 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    12061 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    12121 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    12181 cgttgaagcg atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct
    12241 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    12301 tacagagggg cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    12361 ttggctacgg tgattggtag ccgttctggt agttgtgatg gtggtatgaa aaaagtcatt
    12421 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg
    12481 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    12541 tattatgcgc tgggggtggt ggtcggtgcg ccaataatcg cactcttttc cagccgctac
    12601 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    12661 acgctctctt cgtcgtacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat
    12721 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    12781 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    12841 ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt
    12901 tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg cgacgaggcg
    12961 aaaggaaagc tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc
    13021 gccgccacca tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt aaagccatac
    13081 atgatgttta tttccggttt ttcgaaaacg gcgatgacct ttattatgat gttagttggg
    13141 ctagggatgg tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg
    13201 cgcattgcag cagtgactga ctttataatt gtactggcac tgctgatgct ctttttctgc
    13261 ggcggcatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc
    13321 ctttcagcac ctctgcaaat attgttactg caaaatgcca aaggcggaga gttattaggt
    13381 gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt
    13441 atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg
    13501 tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat
    13561 agtccggtgc tggcgaaacc actggggtag attactgtca cggtgtgcgt tgcttattgc
    13621 cggatgcggc gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga
    13681 taagacgcgg caagcgtcgc atcaggcatg atgcgccaat tgcctgggtt ttttactctt
    13741 gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca
    13801 ccggcgtttc acattcgccg ccgaagacaa attgtttaat caactgcgga accgtttgat
    13861 ataaacggtc tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat
    13921 tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg
    13981 ctaccggatc gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc
    14041 tcaaacgacg ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt
    14101 gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg tccatccacg
    14161 gtagcggatt gtgtccccac tcacctgccg tgccattgcc gccgatatgc gcccgcccat
    14221 tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc
    14281 ccgctgccgc gccatctact gcttctgaaa ccgccagaca gttggcgtca tttgccagcc
    14341 gcacttcccg ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg
    14401 ttgaattggc attcttcacc acaccggtgt aaggcgaaat tgagccagga atgcccatac
    14461 ctaccgttcc gcgctgcccc gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt
    14521 caatagtctg ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc
    14581 ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca
    14641 cggtactctc cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa
    14701 tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa
    14761 ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc
    14821 gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg
    14881 acatggcgaa gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg
    14941 ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga aaaaatccta ccgtctccgg
    15001 tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc
    15061 tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ttgccgcgtg
    15121 ctttcagccg ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg
    15181 tggactgcgc cagtgccaaa aaagcggaag atacgctggc actgctgcgt aaaagcctgg
    15241 ggtcgttacc ggttgtacca ttgagcatgg caaacccgat tgaactgacg ctgaccgaat
    15301 gggtacgttc cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat
    15361 cgttgctgga agatggcgga gtgatccgcg cgaagaaaca agatctgacc agcgaagaga
    15421 tcaccaatca cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc
    15481 gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tctgaagttc tgcgacgagc
    15541 tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt
    15601 tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg
    15661 gcgaagcaca acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc
    15721 ttatccggcg tacgaagcca caccaggcat ataattattc gctacggcgg gcaataattt
    15781 tttagcgcag caatattatg cgttttacgc cgtaactgat ctaatcgcct catccctatt
    15841 cgcctgtcag cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt
    15901 gttaacaact ccgcttttgg ggtgtcaata tattcacgca ataaatcaga aattgaacaa
    15961 atatccgtca ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccaccgttg
    16021 cctgcaataa agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa
    16081 cgcggtaaat cggtgcttcc accattttgg ctgcgcatgg gataacgggt acagtgaatt
    16141 tgcaggttga tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccagggg
    16201 cgttgtgtca ttgtttttcc tcaagccggt gacgcgcttc attgcgaatt ttccgcgttg
    16261 ccccggtttt cactaaatcc cgtaactgcg aaagatcggg tttatcgaca aataaaagta
    16321 atgatggatc ctcttttagc cacaccgttt tcacatcggc agccagctgt ggattattaa
    16381 tagccagcga ccgatcctga gactcagtca atgtcgttaa caacgacgcc ggggtatgtg
    16441 cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc
    16501 attcatcatc tgatttatct tttatcgtcg ccagttccgc aaaaatatcg ctatcctcat
    16561 ggttccagcg gtgtaatcct ctttcacttt gtctacgggt gctatttttc atactgacaa
    16621 cagccagcgc ccagtcagag ttgaagcgat actgttgatc gagtttgttg ataattccgg
    16681 aaggtaattc tttattatca aataattggt tacgcattcg atagcggtat tcagcgggta
    16741 aatcaaccag taaatcaatg gcattattta acatctcttc gtcaacaaca ccattatcaa
    16801 tcagcgccag ctgtacttcg taggcttgct cttgatcttc ttgccgtttg ctcatcatta
    16861 aatttaatgt gcgttgccat aactgtttct tatattccag cggcaataat ttatcgcgtg
    16921 ccagctcatc ccagacggtg ggaacatcat gctgagtcaa tagataacta atcacgtcct
    16981 tcgatgtcag ataacgcgca ccttcgcgca aattgtgcac atcttctttt gccagagaca
    17041 gttgacgcat ctccggcaac gctatcaata acacctttac gatattttta ttttctttat
    17101 tatccagata catttgttct gcgatacgtt caatatcctc tgtacgcagg gttgctgaca
    17161 gttgggtaat ttttaattca gtaagcgtcc gggctaatgc ttcacgaact ttcagtgatt
    17221 tatctttact caacatcact gcttgttgtg cggttaaatc tgctccggta attctttcac
    17281 gtaactccgg gtcttcgtta tttatcaact catcggccac tgcagtcgga accagccagg
    17341 cgtaatcgta atggctgagt aatgtcttgc gtacactgag aaaggggcta tgacttaacg
    17401 acgcaacaat atctggagaa agattatcct gtctggcctg ctccagctgt acgctctcga
    17461 cgggtacaga cattaatttt ttattcacat cggcggaaat attactgttt ttaaccagcc
    17521 ctctggcaac agcctcatct ttaattaatt catcggtaag ccaatgcatc tgttgagcgg
    17581 gccaggactg cgccatcgat tcacgcgtct ggtcatcggg gtgctgtaac agttgcagca
    17641 taacggcggg tgataaactc tctgtatgag tcagaaaacg tgccaccagt ggatcttgat
    17701 gctttatttt attcgctggg aaattatcga gtttccccgt ttcaatccat tttaatttct
    17761 cattaatttc aggagcggct ttatctcgaa aaagcggtaa accctgaacg atgttgtctg
    17821 cgacgctatc atagtcagga tacgcattgg aaatgttatc ggtttcgatt tcagtgtgat
    17881 cataaagatc ggcagtcacc agtgaatagg ctttttgaat attctcatcc cagttatatc
    17941 cttcaccact tccatcttca ttgatataaa attcagcttt aatgacttca aattgcgttg
    18001 ctttatcgga aacaataatg cctcgcgcac taattccctt catcacttca aagttatgat
    18061 cattgtaatc agcataaact aaacctttcg ctgtcatctg tcctttgaca tacgcgaaat
    18121 catgattaat aaagttatcg acaataacat tacctaatac aatcagatga ccattacctg
    18181 acagataatc atcataatcg ccggaaatcg ttaaattacc gttcaccacc agtttatttt
    18241 tattgctgaa gttagcgggt aatgataggt caccattatg ataataccag cggatattat
    18301 cttttagcat ataagcagct tccatactat cgtcggttgc cgctttaacc acgttgcgct
    18361 catctgccgt cataagatga aagtcagagg tgataaggtc ctcgctactg atccactctg
    18421 tggcacatgc attgccagtc ataaaaaata agaccataca gatgaccaat atattaattc
    18481 tattcataat cttcaatgat atttatatca accttgatga attatgaata tatcggaaat
    18541 tgaaaaaatc gcagcaaagg aaatattatt atggggttat gagaggacat ttcagaaaaa
    18601 tgccctcccg ttcaagcgga aggcgaggaa ttacagatag cggcacagat aagaggtggg
    18661 ttcggcaact tgcagatgaa actcactgtg accgggaaca ttaaacaccg aaccggcttc
    18721 atacacttgc cagtcggtcg cgtcaggcag taacacattc agcgcgccac tgattaccgt
    18781 catctcttcc ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa cacccacgct
    18841 ggcgcgacca gtgctgctgc tggaaaagcc gatcgatttc actttgccgg aaaagtactc
    18901 attactttga agcataaact ggccctgtct ggtagttgaa tatctacttc actatagggg
    18961 ccagatgcgc tggctgtcac gcaaaattac accagtaatt ccgcagccag ccgtgcaatc
    19021 aatacgttag agagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgc
    19081 tggttaaatc ccaaacaatc cagcatgatg acatctgcgc ctttcgccag taattctttc
    19141 ccggcatcaa tgattttttg ttctgaatca tgaatggggt tacccaatga aaatacaggc
    19201 ggtttctgca aaatttgcca tttttgcgcc tgaacggtca gcaactcctc aaccggtacg
    19261 ataaccccca ccagatgatc ttcaacaata gaggaaacca gcggtggcaa tatccgcgac
    19321 ggctcaagaa agatcgtatt acgcgcagtc atactactaa tgtttgccgt actcattaat
    19381 ataatgacgt cgtagccctg attatcgagc acttcaacca caccttgcag gtcacgctct
    19441 actttgcgac gcgaaacatg ggccagatgg ttgtcattta ataatgtgag aattgtgtct
    19501 tcgcctgctt ctggcgcgta ctccgccatc acttcttcgc ggcttaactt ccccagcagg
    19561 ctatgatggg aaatattatc ttcgtcaatg tattctgtca ggagcggcaa aacttcctgc
    19621 ataggtacaa tgccgatggt tagaatcgcc aacgacgcac tcattgttct tccaccttac
    19681 ttttatttcg tcgttaatta ccgggcaagt gttaagcacc actgctgaca cttacttgtg
    19741 ctacaaatga caaaaagcgt agcagcagac gtgcacggca taatgtaaag attccaaatg
    19801 attccagtaa tggattgtta ttgtttaata ttctaattat ttgaaaaaca tgaattatga
    19861 aaaatgtgac gcagatccct tgtagtggca ctcagaatcc cttccgagca gtctgatgcg
    19921 gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagcggtcg
    19981 gatgttcact gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta
    20041 ccgtctgtcc tggctttcct ttttcaattg tcactatata tgcttcacga ggataaggcg
    20101 gtttcgttgg catcgttctt ccttatttca cgggatgaac gttaagtata ggcgctcgaa
    20161 aatcaacaat tgatcgtctg cgccagggcg ctgcgaattt cagaaatcac ctggctgggt
    20221 tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc
    20281 agcacttcct gaacttcttc gctcagcggt tttcccgtta aggttggccg taaatcttct
    20341 tccggtgcag cttgcagtcg gttaaccagg actgatactg gcgcacacaa ataaaccacg
    20401 atcccgttat tttgcatgaa gtgacgatta aattccgtca gaataatgcc gccgcctgta
    20461 gcgataacgg tggatggcgc agttaccgct tccagcgccg ccgtttctct ggcgcgaaat
    20521 cccgcccact cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac
    20581 cactgatcgg tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt
    20641 gttttaccac agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc
    20701 caataggtcg caatgcggcg aaagccggtg tcatgagaat agcgatcata ccatcaaact
    20761 agtacaattt cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca atacatccct
    20821 tactatacca ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac
    20881 tacgttgcac ttccagccac cacttctcca gctccgcggc aaaggcctgg cggtcacgtt
    20941 gtgaaaggct atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat
    21001 cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcaccg cgcggattaa
    21061 gcgccgcagc gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca
    21121 aatctcccgc ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga
    21181 cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta
    21241 ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac
    21301 acgcgtcggc atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg
    21361 tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac
    21421 aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc
    21481 aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa
    21541 atctgggtat ataccccctc cccggataaa gtcgccgccc tgcatgacaa gttcggcatc
    21601 aacgccgcag aatcggcgca agaagtggcg caaatcgccg acatcatttt tgccgccgtt
    21661 aaacctggca tcatgattaa agtgcttagc gaaatcacct ccagcctcaa taaagactct
    21721 ctggtcgttt ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat
    21781 gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc
    21841 tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc
    21901 cgctgctttg gcgaagcgga agtaattgct gagccgatga tccacccggt ggtcggtgtg
    21961 agcggttctt cgccagccta cgtctttatg tttatcgaag cgatggccga cgccgccgtg
    22021 ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc
    22081 gcaaaaatgg tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca
    22141 ccgggaggca ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgccgca
    22201 gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga
    22261 ctttcgccgg acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt
    22321 agagcgccaa atctgccgat ttcaaccact cgcgatagtg actcatttgt gggttcagcg
    22381 gcgcaacccc cacactaatc cgtaaattta cctgtggcgt attcggcaaa cgtaatgtat
    22441 ttagcccttc atgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca
    22501 tgattaccgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa
    22561 tttgtaactg tcgggtaagc gccacaatcg cttcatcgcc cacatcatgg ccccaggtat
    22621 cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc
    22681 gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc
    22741 ccgtcatgcc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttgg
    22801 ttgccgtctg gtagctgacc cagccaaaca gcagaggata aatgacaata atgggaaggg
    22861 agagccacca ttccagcggc gcactattga acgacacggt aatgcccgtc agctcgaggg
    22921 tgacaaggca ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg
    22981 cccccatcaa attcagacac ataatcatca acatcgcggt ggaaggcagc acgtttacgc
    23041 ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc
    23101 ggctaagcgg atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg
    23161 cccagccgac caacaccagc caccaccagc ccggcggcgg gtgtgaaacc agcgttgaag
    23221 caatcggtaa gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga
    23281 agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccgtgcg
    23341 ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat
    23401 tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccattt
    23461 tagtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcgctcat
    23521 aaacttatta cgatcatcac ctttcagtga ttgttgcgtt gcttgattat tacattcgcg
    23581 catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag
    23641 gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgtgccg tcgcctgctg
    23701 attacaggag gtcatacgct gttgttgtgg ggttaaggtt ctctcggcag cgccgacggt
    23761 ggttaaaaaa accagaccga aaagcaaggt aaccagtaat gtaattttca tagcaccatc
    23821 cctcttcatg ttttaaccat gagcgtatgc gcccgtgatc tgacattaag tctggttgct
    23881 aacagcaaaa aaaccacccg gcagcgaaaa ttcactgccg ggagcggttt tatttcagcc
    23941 ccagagcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca acgacattgg
    24001 cggcatgcgg gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct
    24061 cttcggagtt cccgtaactc atcaccatca ctgcgccatc tttggtattt agcgcctggg
    24121 tgaggcccgg agctttggta tccggcgcaa caatctggct ggcgtgggcg tgatcagcgg
    24181 tgactatgac cagcgtgtta ccatcctttt tggcgaattc cagcgcccgt tgtacggctt
    24241 catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcatga tcctgtttat
    24301 cgattgacgc accttcaact tgcaggaaaa agcctttctc atttttactc aacaattcaa
    24361 tggctttgtc ggtcatctgt gccagggtcg gtacactgtc attacgttgc ggattaggcg
    24421 tacaggtgac tgcgggctta tcgatattac cgtggtacgt tgctttcggt cctagccagc
    24481 gcactggcat attgccgtca gcaaacaggc caagcagggg tttttgctga ttcgcttccg
    24541 tcaccgaatt cagtgaggca gcatcgctca ccaactgata accacgcgcc tgtgcctgtt
    24601 cacgcagcgt ttttccctgc cattcaccag cggttgccgt ttcagcaaag gtttttgcgc
    24661 cgccgccaag cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc gatccttttc
    24721 cgcctttttc cagagcgtta cccggacatt tttcactggt cgcgctcgga ccgtagcatt
    24781 tgcgcgaggt cacatgcgcc accagcgcgg cgggcgtggc atcctgcaac tctgcggtag
    24841 aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt
    24901 tttcgtgaat atcgacgccc agcgcgccgt tataggtttt gacaccggtt gaccaggcgg
    24961 ttgctgatgc agccgagtcg gtgacgtagt ccggttttcc ggttttttta ttcagcgcat
    25021 agtgagtgta ttgcccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac
    25081 cttcggcata attacgtgcg gcagtaattt ccgaatcccc catcccatcg ccaatcagca
    25141 aaataatatt ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcag
    25201 ccgttaagcg gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca
    25261 gaacaggcat ttctggtgtc cgggcttttg tcacaggggt aaacagtaac ggtaagagtg
    25321 ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata
    25381 aaaacaaagc gactataagt ctcggccgtg acaactttat gacagctgtt gaaaagatta
    25441 actttttact gacgaggatg cttcaataac ttctttacgt aatcgcgcag cagctccgta
    25501 tcgtcgtcag gaatgctggc atcgggcttt acctcgtaca gcgccccctc tacctgatca
    25561 atcaaccgct gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac gataatctcc
    25621 aaagcttcta cctgcgcaca cagttctttc gactcttctt ctttttgggc aagcttaaat
    25681 aacaactcag caatgagatt tttcatgtct gtatttcctt atccaaagta tggagaagtt
    25741 aacattatgt ttcacaactt catagtgtct ggaggtatta tttttatggc aaaggaaata
    25801 ctttgcgcaa atattttttg tttgattacc agcgaaacgt ttctcttatt taaa
//
LOCUS       DAZGXS010000063        25726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGXS010000063 DAZGXS010000000
VERSION     DAZGXS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            382..1521
                     /gene="wza"
                     /locus_tag="ACHKG5_002669"
     CDS             382..1521
                     /gene="wza"
                     /locus_tag="ACHKG5_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP0868750.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1527..1970
                     /gene="wzb"
                     /locus_tag="ACHKG5_002670"
     CDS             1527..1970
                     /gene="wzb"
                     /locus_tag="ACHKG5_002670"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0868751.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1973..4135
                     /gene="wzc"
                     /locus_tag="ACHKG5_002671"
     CDS             1973..4135
                     /gene="wzc"
                     /locus_tag="ACHKG5_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0868752.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4228..5067
                     /gene="wcaA"
                     /locus_tag="ACHKG5_002672"
     CDS             4228..5067
                     /gene="wcaA"
                     /locus_tag="ACHKG5_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0868753.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5070..5558
                     /gene="wcaB"
                     /locus_tag="ACHKG5_002673"
     CDS             5070..5558
                     /gene="wcaB"
                     /locus_tag="ACHKG5_002673"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0868754.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5555..6772
                     /gene="wcaC"
                     /locus_tag="ACHKG5_002674"
     CDS             5555..6772
                     /gene="wcaC"
                     /locus_tag="ACHKG5_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP0868755.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6747..7964
                     /gene="wcaD"
                     /locus_tag="ACHKG5_002675"
     CDS             6747..7964
                     /gene="wcaD"
                     /locus_tag="ACHKG5_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0868756.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7975..8721
                     /gene="wcaE"
                     /locus_tag="ACHKG5_002676"
     CDS             7975..8721
                     /gene="wcaE"
                     /locus_tag="ACHKG5_002676"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0868757.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8737..9285
                     /gene="wcaF"
                     /locus_tag="ACHKG5_002677"
     CDS             8737..9285
                     /gene="wcaF"
                     /locus_tag="ACHKG5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0868758.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9314..10435
                     /gene="gmd"
                     /locus_tag="ACHKG5_002678"
     CDS             9314..10435
                     /gene="gmd"
                     /locus_tag="ACHKG5_002678"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0868759.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10438..11403
                     /gene="fcl"
                     /locus_tag="ACHKG5_002679"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10438..11403
                     /gene="fcl"
                     /locus_tag="ACHKG5_002679"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0868760.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11406..11885
                     /gene="gmm"
                     /locus_tag="ACHKG5_002680"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11406..11885
                     /gene="gmm"
                     /locus_tag="ACHKG5_002680"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0868761.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11882..13105
                     /gene="wcaI"
                     /locus_tag="ACHKG5_002681"
     CDS             11882..13105
                     /gene="wcaI"
                     /locus_tag="ACHKG5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP0868762.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13108..14544
                     /gene="cpsB"
                     /locus_tag="ACHKG5_002682"
     CDS             13108..14544
                     /gene="cpsB"
                     /locus_tag="ACHKG5_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP0868763.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14737..16107
                     /gene="cpsG"
                     /locus_tag="ACHKG5_002683"
                     /gene_synonym="manB"
     CDS             14737..16107
                     /gene="cpsG"
                     /locus_tag="ACHKG5_002683"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0868764.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16162..17556
                     /gene="wcaJ"
                     /locus_tag="ACHKG5_002684"
     CDS             16162..17556
                     /gene="wcaJ"
                     /locus_tag="ACHKG5_002684"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP0868765.1"
                     /translation="MTNLKKRERTKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17558..19036
                     /gene="wzxC"
                     /locus_tag="ACHKG5_002685"
     CDS             17558..19036
                     /gene="wzxC"
                     /locus_tag="ACHKG5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP0868766.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19216..20496
                     /gene="wcaK"
                     /locus_tag="ACHKG5_002686"
     CDS             19216..20496
                     /gene="wcaK"
                     /locus_tag="ACHKG5_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP0868767.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20493..21713
                     /gene="wcaL"
                     /locus_tag="ACHKG5_002687"
     CDS             20493..21713
                     /gene="wcaL"
                     /locus_tag="ACHKG5_002687"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP0868768.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21724..23118
                     /gene="wcaM"
                     /locus_tag="ACHKG5_002688"
     CDS             21724..23118
                     /gene="wcaM"
                     /locus_tag="ACHKG5_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP0868769.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23276..24271
                     /locus_tag="ACHKG5_002689"
     CDS             23276..24271
                     /locus_tag="ACHKG5_002689"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP0868770.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24514..25407
                     /gene="galF"
                     /locus_tag="ACHKG5_002690"
     CDS             24514..25407
                     /gene="galF"
                     /locus_tag="ACHKG5_002690"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP0868771.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6362 a         6365 c         6935 g         6064 t
ORIGIN      
        1 ttaccttgcc tgctattccg ttagctgtaa cacttcctcc tgcattattg gagagccaat
       61 attcaattac cataatgtca ccttatattc ggaagttaat ttccgcaata accctttctc
      121 atcaacgatt gtatgcagag agggtttgaa aaattaagct aaatcagtgt attggtagct
      181 aaaaagccag gggcggtagc gtgtctggat gcctgaaaga ccagtctgaa ttattctgtc
      241 aatagcctgc ggatgaaatc aattttttta ggactgatgc cagttaaatt tttattcaat
      301 taactgcaag gtaaatgtgc atggcgacat tgctattaat agtgcacagg ataattactc
      361 tgccaaagtg ataaataaac aatgatgaaa tccaaaatga aattgatgcc attattggtg
      421 tcagtaacct tgataagcgg ttgcacagta cttccgggca gcaatatgtc gacgatgggc
      481 aaagacgtca tcaaacagca ggacgctgat ttcgatctcg acaaaatggt gaatgtttat
      541 ccgctgaccc cgcgcctgat tgaccaatta cgcccacgcc cgaatgtagc gcgccccaat
      601 atgacgctgg aaagtgagat cgcgaattac cagtatcgcg tcgggccggg ggacgttctt
      661 aatgtcaccg tctgggatca cccggaactc accacgccag ccggtcagta ccgcagctcc
      721 agcgacaccg gcaactgggt acagcctgac ggcactatgt tttacccgta tatcggcaag
      781 gttcacgtag tcgggaaaac gctcgctgaa atccgcagtg atattaccgg gcgcttagcg
      841 acgtacatcg ctgacccgca ggtggacgtt aatatcgccg ccttccgctc acaaaaggcc
      901 tatatctcag gtcaggtgaa taaatctggt caacaggcga tcaccaacgt accactgacc
      961 attctcgacg ccatcaacgc cgcaggtggc ctgaccgaca ccgctgactg gcgcaacgtg
     1021 gtgctaacac acaatggtcg tgaagagcga atttctttgc aggcgctgat gcaaaacggc
     1081 gacctcaatc agaaccgact gctttacccc ggcgatattc tctacgtgcc gcgtaatgat
     1141 gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga gcaccctgaa aatggacttt
     1201 agcggcatga ccctgaccga agccctgggc aatgctgaag gcatcgacat gaccacctcc
     1261 aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg agggcgggcg taacggcaag
     1321 attgccaata tctaccagct ggatatgtcc gatgccacgt cgctggtgat ggcgacagaa
     1381 ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg ccccggtttc ccgctggaac
     1441 cgtctgatca atcagttgct gccaactatt agcggtgtcc gttacatgac ggatacagcc
     1501 agcgacattc ataactggta atcgtcatgt ttaacaacat cttagttgtc tgtgtcggca
     1561 atatttgccg ttccccgacg gcggaacgct tactgcaacg ttatcacccg gagctgaaag
     1621 tggagtccgc tggactcggc gcgctggtcg gtaagggcgc tgatcctacc gctatcagcg
     1681 tcgccgcaga acatcaactg tctctggaag gccactgtgc acgacaaatc agccgccgcc
     1741 tgtgtcgcaa ctatgacctg attttgacca tggaaaagcg ccatatcgaa cgcttatgcg
     1801 agatggcacc ggagatgcgc ggcaaagtga tgctgtttgg tcactgggat aacgaatgtg
     1861 aaatccccga tccgtatcgc aaaagccggg aaacgtttgc agcggtgtac acattacttg
     1921 aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga gcaggtataa gaatgacaga
     1981 aaaagtaaaa caacatgccg ctccggtaac gggcagtgat gaaatcgata ttggtcgcct
     2041 ggtcggcacc gtcattgaag cgcgctggtg ggtgattggc atcaccgctg tattcgccct
     2101 ttgtgccgtg gtttacacct tcttcgccac gccgatttat agtgccgacg cactggtaca
     2161 aatcgagcaa agcagcggca attcgttagt gcaggatatc ggatcggcgt tagccaacaa
     2221 accgcctgca tcggacgccg agatccagtt gattcgttcg cgcctggtgc ttggtaaaac
     2281 ggtggatgat ctcgacctcg atattgcggt gagcaaaaac acgttcccga ttttcggtgc
     2341 gggctgggat cgcctgatgg gacgccagaa cgagacggtg aaagtgacta cctttaaccg
     2401 tccgaaagag atggaggatc aggtgtttac gcttaatgtg ctggacaaca aaaactacac
     2461 cctgagcagc gatggcggct ttagcgcccg tgggcaagcg ggccagatac tgaaaaaaga
     2521 aggcgtcacg ctgatggttg aagccattca cgcccgcccg ggcagtgagt ttaccgtcac
     2581 caaatactcc acgctgggga tgatcaatca actgcaaaac agcctgacgg taacggagaa
     2641 cggcaaagac gcaggcgtac tgagcctgac ttataccggt gaagatcgcg aacagatccg
     2701 cgacattctt aacagcatcg cccgtaacta tcaggaacaa aatattgagc gcaaatcggc
     2761 ggaagcgtcg aaaagcctcg ctttcctcgc gcaacagtta ccggaagtac gtagccgcct
     2821 tgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat aaagattctg ttgatctgcc
     2881 gctggaagcg aaagcggtgc tcgattcgat ggtgaacatc gacgcccagt tgaacgaact
     2941 gacctttaaa gaggcggaaa tctccaagct gtacaccaaa gttcaccccg cgtaccgcac
     3001 gctgctggag aaacgtcagg cgctggaaga cgaaaaagcc aaacttaatg gtcgcgtaac
     3061 ggcgatgccg aaaacccagc aggaaattgt ccgtctgacc cgcgatgtcg agtctggtca
     3121 gcaggtctat atgcaactgc tgaataaaga gcaggagctg aaaatcaccg aggccagcac
     3181 cgtcggcgat gtgcgcattg ttgacccggc aatcactcag cctggtgtgc taaaaccgaa
     3241 gaaagggctg attatccttg gggcgattat ccttggcctg atgctctcta tcgtgggggt
     3301 gctgctgcgc tcgttgttta atcgcggcat cgaaagcccg caggtgctgg aagaacacgg
     3361 tatcagcgtc tatgccagca tcccgctgtc ggagtggcag aaagcgcgcg atagcgtcaa
     3421 aaccatcaaa gggattaaac gctataaaca gagccagcta ctggcggtgg ggaatccaac
     3481 cgatctggcg attgaagcca tccgcagcct tcgtaccagt ttgcacttcg cgatgatgca
     3541 ggcgcagaac aatgtgttga tgatgaccgg ggttagcccg tcaatcggta aaacctttgt
     3601 ctgcgccaac ctggcggcgg tgatcagcca gaccaataaa cgcgtgttgt tgatcgactg
     3661 cgatatgcgc aaaggctaca cccatgagct gttgggcact aataacgtta atggcctgtc
     3721 ggaaattctg attggtcagg gcgatattac tacagctgct aaaccgacct ctattgccaa
     3781 atttgacctg atcccgcgcg gtcaggtacc gccaaatcct tctgaactgt tgatgagcga
     3841 acgctttgcc gaactggtga actgggcgag taaaaactac gacctggtgt tgattgatac
     3901 gccgccgatt ctggcagtga ccgatgcggc aattgttggt cgtcatgtcg gaaccacgtt
     3961 aatggtggcg cgttatgcgg tcaacacatt gaaagaagtg gaaaccagtc tgagccgctt
     4021 tgagcaaaac ggtattccgg tgaaaggggt gattctgaac tccatcttcc gccgcgccag
     4081 cgcgtatcag gattatggct attacgaata cgaatataag tcggatgcga aataacgagg
     4141 cctgcattcg caccgccccg taggccggat aaggcgctca cgccgcatcc ggcaagcaaa
     4201 ccagctcata agccgggagt acaacctatg aaagacaatc cgctgatctc aatctatatg
     4261 ccgacctgga accgccaaca actggcgatt cgggcgataa aatcggtctt gcgccaggac
     4321 tacagcaact gggagatgat catcgtggat gattgctcca cctcctggga acaactgcaa
     4381 cagtacgtca ccgccctcaa cgatccgcgt attacctaca ttcacaacga cattaacagc
     4441 ggggcgtgcg cggtacgtaa ccaggcgatt atgctggcgc aaggggaata cattaccggg
     4501 atcgatgacg atgatgaatg gacgcccaac cgcctgagcg tcttcctcgc ccataaacag
     4561 caactggtca ctcacgcctt tttgtacgct aacgattacg tttgccaggg cgaagtttat
     4621 tcccaaccgg caagcttacc actgtatccc aaatcgccgt actcacgccg cctgttctat
     4681 aaacgcaata tcattggcaa tcaggtcttt acctgggcat ggcgtttcaa agagtgtttg
     4741 ttcgataccg aactgaaagc ggcacaggat tacgacatct tcctgcggat ggtggtggag
     4801 tatggcgaac cgtggaaagt ggaagaggcg acgcagatcc tgcatatcaa tcacggtgaa
     4861 atgcagatca cctcgtcacc gaaaaaattc tccggatact tccactttta ccgcaagcac
     4921 aaagacaaat tcgaccgtgc cagtaaaaaa tatcagctgt ttaccctcta ccagatccgc
     4981 aataaacgta tgacctggcg cactttgctg acgctgctgt cggtgcgtaa cggcaaacgt
     5041 ctggctgatg gtattcgggg gcgctaaata tgctggaaga tctacgcgcc aacagctgga
     5101 gtttacgccc gtgctgcatg gttcttgcct atcgcgtcgc tcatttttgt tcggtgtggc
     5161 gcaaaaagaa cgttctcaac aatctttggg cggccccgct gctggtgctg tatcgcatta
     5221 tcaccgaatg ctttttcggt tatgaaatcc aggctgccgc gaccattggc cgccgcttta
     5281 ctatccatca cggttacgcc gtagtgatca ataaaaacgt ggtagcgggg gatgatttca
     5341 ccattcgcca cggcgtcact atcggcaatc gtggtgccga taacatggca tgtccacaca
     5401 ttggcaacgg cgtcgaactc ggtgccaacg tcattattct tggtgatatc acgcttggta
     5461 acaacgtcac cgtgggcgcg ggcagcgtgg tgctcgactc tgtcccggac aacgcgctgg
     5521 tggtgggaga aaaagcgcga gtgaaggtaa ttaaatgaat attctgcaat ttaatgtgcg
     5581 actggcggaa ggcggggcgg caggtgtggc gttagatctc caccagcgtg cgctgcaaca
     5641 ggggctggcg tcacattttg tctacggcta cggcaaaggc ggcaaagaga gcgtcagcca
     5701 tcagaactat ccgcaggtca tcaaacatac gccgcggatg accgcaatgg cgaatattgc
     5761 cctgtttcgt ctgtttaatc gcgatctgtt tggcaatttc aatgagttat atcgcaccat
     5821 tactcgtaca ccgggtccgg tggtcctgca ttttcatgtg ctgcacagct actggctaaa
     5881 tcttaagagc gtggtgcgct tttgcgaaaa ggtgaaaaac cacaaaccgg atgtcactct
     5941 ggtctggacg ctgcacgacc actggagcgt taccgggcgc tgcgccttta ccgacggttg
     6001 tgaaggctgg aaaacgggct gccagaaatg cccgacctta aataactatc cgccggtgaa
     6061 gattgatcgg gcgcaccagc tggtggcggg caaacgccag ttattccgtg agatgctggc
     6121 gctgggctgt cagtttattt cccccagcca gcatgtggct gacgctttca atagcctgta
     6181 cggtccaggg cgttgccgga ttatcaataa cggtattgat atggcaaccg aggcgattct
     6241 ggcggatttg cctccggtgc gcgaaactca gggcaagccg aaaatcgcgg tggtagcgca
     6301 tgacctgcgt tacgacggca aaactaacca gcaactggta cgcgagatga tggcgctggg
     6361 cgacaaaatc gaactgcata cctttggtaa gttctcgccg ttcaccgctg gcaacgtggt
     6421 taatcacggc tttgaaactg acaagcgcaa gttgatgagc gcgctcaatc agatggatgc
     6481 gctggtgttc agttctcgcg tcgataacta cccgctgatt ttgtgtgagg cgttatcaat
     6541 tggcgtgccg gtgattgcca cccatagcga cgcggcgcgt gaagtgttgc aaaaatccgg
     6601 cggtaaaacc gtcagcgaag aagaggtgct gcaactggtc cagttaagca aaccggaaat
     6661 cgcgcaggcg ttatttggta ccacgctggc tgagttcagc caacgcagcc gcgccgccta
     6721 cagtggacaa cagatgctgg aggagtatgt caacttctat cagaatctgt agctacctgc
     6781 tgctgccgct gatctacctg ctggttaacg tcaaaatcac ccagcttggc gaaagtttcc
     6841 ccatcaccat cgtcactttt ttacctgtct tgttactgct gtttttagaa cgcatcagcg
     6901 ttaaaaaatt gatgattgcc ttagggattt gcgcgggact cacggcgttt aactacctgt
     6961 ttggtcagtc gctggatgcc agtaaatacg tcacttcaac tatgctgttt gtctatatcg
     7021 tgatcattat tggtatggtg tggagtattc gctttaaaac gatttcgcca cacaaccatc
     7081 gcaagatatt acgtttcttt tatctggtgg tcgggctggt ggtggcgctg gcggcggtgg
     7141 agatggcgca aattatcctc accggcggca gcagtattat ggagtcgatt tcgaaatatc
     7201 tgatttacag caacagctat gtgctgaatt tcatcaaatt cggcggcaag cgcacgacag
     7261 cactttattt cgaaccggca tttttcgctc tggcattaat ctcaatttgg ctcagcatca
     7321 aacagtttgg tatcaaaacg cctaaaacag atgctatgat tctcgcaggg ataatattat
     7381 ccggatcgtt ttcaggggtt atgaccttta tcctgtttta tttgctggag tgggcatttc
     7441 aatatctgaa taaagaggcg attaagaaaa agttaccgtt agcattgatt tctctggctg
     7501 tattcctggt tggtgtggta attgcatttc cttatatttc cacccgtctg ggcgatttag
     7561 gtacggaagg atcgtcatcg tattatcgta ttgtcggtcc gctggtgatg gtcggttatt
     7621 ctttgaccca tattgacggt gtagtcagat ttggctcact ttatgaatat gtcgcatcat
     7681 tcggaatatt taacggtgcg gatgtcggaa aaaccataga caatggtttg tatctgctga
     7741 ttatttattt ttcttggttc gcggtgtttt tatcgctgtg gtacatgggg aaagtgataa
     7801 aaatgatgat caacgctttt ggtgataacc gcaattttcg cgtgcaatta tatcttttta
     7861 ctccggtatc gctgtttttt accggttcga tatttagccc ggaatatgca tttttaatcg
     7921 tctgtccgtt tattttgcga aaagcgttaa atattacgag gtaagaataa gaacatgttg
     7981 cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga tagtcaaaac acatgcctcg
     8041 ctggcgcatc tggcgcaggc ggaagatatc agcttcgaat ggattgttgt cgatggcggt
     8101 tccaatgacg gcactcgtga gtacctggaa aatctcaatg gtatctataa cctacgcttt
     8161 gtcagcgagc cagataacgg tatctacgac gccatgaaca agggcattgc gatggcacaa
     8221 ggcaagttcg cgttgtttct caactcgggc gatatttttc atcaggatgc cgcatatttt
     8281 gtccgtaagt taaaaatgca aaaagataac gtgatgatca ccggcgatgc gctgctggat
     8341 tttggcgacg ggcataaaat taaacgtagc gccaaaccgg gctggtatat ttatcacagc
     8401 ctgcccgcca gtcatcaggc gatatttttc ccggtatccg gtttgaaaaa atggcgttat
     8461 gacctggaat ataaagtttc ttctgattac gcgctggcag ccaaaatgta taaagcaggt
     8521 tatgcattta aaaaactcaa tggcctggtg tctgaatttt ctatgggtgg ggtatctacc
     8581 accaataata tggaattgtg tgctgacgcg aaaaaagtcc aacggcaaat attacatgtg
     8641 cctggctttt gggctgaatt atcctggcat ttacgccagc gtactacctc aaagacgaaa
     8701 gccttatata acaaaagctg aatataaggg aaaacgatgc aggatttaag cggtttctcg
     8761 gtgccgaaag ggttccgggg cggcaacgct attaaagtgc aattgtggtg ggcagtacag
     8821 gcaacaatat ttgcctggtc gccacaagta ttgtatcgct ggcgggcatt tttattacgt
     8881 ttattcggcg caaaaatagg aaaaaacgta gttattcgtc cgtcagtaaa aattacctat
     8941 ccgtggaaat taaccttagg tgattacgtg tgggtcggcg atgacgtcaa tttatatacc
     9001 ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc aaaaaagtta tttatgcacc
     9061 gggagccacg accatgcaag tcaacatttc accattaacg ccacgcctat tgtgattggc
     9121 gagaaatgct ggctggcaac cgatgttttt gttgccccag gcgtcacgat tggcgacggc
     9181 accgtcgtgg gtgcgcgaag cagtgttttt aaatcgcttc cggcaaatgt ggtttgccgg
     9241 gggaatcccg cagtggtgat acgcaaacgc gttgaaactg aataaattca aaaaaaatac
     9301 agaggaataa gacatgtcaa aagtcgctct catcaccggt gtaaccggac aagacggttc
     9361 ttacctggca gagtttctgc tggaaaaagg ttacgaggtg catggtatta agcgtcgcgc
     9421 atcgtcattc aacaccgagc gcgtggatca catttatcag gatccgcaca cctgcaaccc
     9481 gaaattccat ctgcattatg gcgacctgag tgatacctcc aacctgacgc gtattttgcg
     9541 tgaagtgcag ccggatgaag tgtacaacct gggcgcaatg agccacgttg cggtttcttt
     9601 tgagtcaccg gaatataccg ctgacgtcga cgcgatgggt acgctgcgcc tgctggaggc
     9661 gatccgcttc ctcggtctgg aaaagaaaac ccgtttctat caggcttcca cctctgaact
     9721 gtatggtctg gtgcaggaaa ttccgcagaa agagaccacg ccgttctatc cgcgctctcc
     9781 gtatgcggtc gccaaactgt atgcctactg gatcaccgtc aactaccgtg aatcctacgg
     9841 catgtatgcc tgtaacggta ttctcttcaa ccatgaatcc ccgcgccgcg gcgaaacctt
     9901 cgttacccgc aaaatcaccc gcgcaatcgc caacatcgcc caggggctgg agtcgtgcct
     9961 gtacctcggc aatatggatt ccctgcgtga ctggggccat gccaaagact acgtaaaaat
    10021 gcagtggatg atgctgcaac aggaacagcc agaagatttc gttatcgcga ccggcgttca
    10081 gtactccgta cgtcagttcg tagaaatggc ggcagcacag ctgggcatca aactgcgctt
    10141 tgaaggcacg ggcgttgaag agaagggcat tgtggtttcc gtcaccgggc atgacgcgcc
    10201 gggtgttaaa ccgggtgatg tgattatcgc cgttgatccg cgttacttcc gtccggctga
    10261 agttgaaacg ctgctcggcg acccgaccaa agcgcacgaa aaactgggct ggaaaccgga
    10321 aatcaccctc agagagatgg tgtctgaaat ggtggctaat gacctcgaag cggcgaaaaa
    10381 acactctctg ctgaaatctc acggctacga cgtggcgatc gcgctggagt cataagtatg
    10441 agtagacaac gcatttttat tgctggccat cgcgggatgg tcggttccgc catcaggcgg
    10501 cagctcgaac agcgcggtga tgtggaactg gtattacgca cccgcgacga gctgaacctg
    10561 ctggacagcc gcgccgtgca tgatttcttt gccagcgaac gtattgacca ggtctatctg
    10621 gcggccgcga aagtgggcgg cattgttgcc aacaacacgt atccggcgga tttcatctat
    10681 cagaacatga tgattgagag caacatcatt cacgccgcgc atcagaacga cgtgaacaaa
    10741 ctgctgtttc tcggatcgtc ctgcatctac ccgaaactgg caaaacagcc gatggcagaa
    10801 agcgagttat tgcagggcac gctggagccg accaacgagc cttacgccat tgccaaaatc
    10861 gccgggatca aactgtgcga atcatacaac cgccagtacg gacgcgatta ccgctcagtc
    10921 atgccgacca acctgtatgg cccgcatgac aacttccacc cgagtaattc gcatgtgatc
    10981 cctgcattgc tgcgtcgctt ccacgaggcg acggcacaga atgcaccgga cgtggtggtg
    11041 tggggcagcg gtacgccgat gcgagaattt ctgcacgtcg atgatatggc ggcggcgagc
    11101 attcatgtca tggagctggc gcatgaagtc tggctggaga acacccagcc gatgctgtcg
    11161 cacattaacg tcggcacggg cgttgactgc actatccgcg agctggcgca aaccatcgcc
    11221 aaagtggtgg gttacaaagg tcgggtggtt tttgatgcca gcaaaccgga tggtacgccg
    11281 cgcaaactgc tggatgtgac gcgcctgcat cagcttggct ggtatcacga aatctcactg
    11341 gaagcggggc ttgccagcac ttaccagtgg ttccttgaga atcaagaccg ctttcggggg
    11401 taatgatgtt tttacgtcag gaagactttg ccacggtagt gcgctccaca ccgcttgtct
    11461 ctctcgactt tattgtcgag aacagtcgcg gcgagtttct gcttggcaaa agaaccaacc
    11521 gcccggcgca gggttactgg tttgtgccgg gagggcgcgt gcagaaagac gaaacgctgg
    11581 aagccgcatt tgagcggctg acgatggcgg aactggggct gcgtctgccg ataacagcag
    11641 gccagtttta cggtgtctgg cagcactttt atgacgataa cttctccggc acggatttca
    11701 ccactcacta tgtggtgctc ggttttcgct tcagagtagc ggaagaagaa ctgttactgc
    11761 cggatgagca gcatgacgat taccgctggc tgacgccgga cgcgctgctc gccagtaacg
    11821 atgttcacgc taacagccgc gcctattttc tcgctgagaa gcgtgccgga gtacccggat
    11881 tatgaaaata ctggtctacg gcattaacta ctcgccggag ttaaccggca tcggcaaata
    11941 caccggcgag atggtggaat ggctggcggc gcaaggtcat gaggtgcggg ttattaccgc
    12001 accgccttac tacccgcagt ggcaagtggg cgagaactat tccgcctggc ggtacaaacg
    12061 agaagagggg gccgctacgg tgtggcgctg cccgctgtac gtgccaaaac agccgagcac
    12121 cctgaaacgc ctgttgcatc tcggcagttt tgccgtcagc agttttttcc cgttgatggc
    12181 gcaacgtcgc tggaagccgg atcgcattat cggcgtagtg ccaacgctgt tttgcacgcc
    12241 gggaatgcgc ctgctggcga aactctctgg cgcgcgtacc gtgctgcata ttcaggatta
    12301 cgaagtagat gccatgctgg ggctgggcct tgccggaaaa ggcaaaggcg gcaaagtggc
    12361 acagctggca acggcgttcg aacgtagcgg actgcataac gtcgataacg tttccacgat
    12421 ttcgcgttcg atgatgaata aagccatcga aaaaggcgtg gcggcggaaa acgtcatctt
    12481 cttccccaac tggtcggaaa tcgcccgttt tcagcatgtt gcagacgccg atgttgatgc
    12541 ccttcgtaac cagcttggcc tgccggataa caaaaaaatc attctttact ccggcaatat
    12601 tggtgaaaag caggggctgg aaaacgttat tgaagcagcc gatcgcctgc gcgatgaacc
    12661 gctgattttt gccattgtcg ggcagggcgg cggcaaagcg cggctggaaa aaatggcgca
    12721 gcagcgtgga ctgcgcaaca tgcaattttt cccgctgcaa tcgtatgacg ctttacccgc
    12781 actgctgaag atgggcgatt gccatctggt ggtgcaaaaa cgcggcgcgg cagatgccgt
    12841 attgccgtcg aaactgacca atattctggc agtaggcggt aacgcggtga ttactgctga
    12901 agcccacaca gaactgggac agctttgcga aacctttccg ggcattgcgg tttgcgtaga
    12961 accggaatcg gttgaggcgc tggtggcggg gattcgtcag gcgctcctgc tgcccaaaca
    13021 caacacggtg gcacgtgaat atgccgaacg cacgctcgat aaagagaacg tgttacgtca
    13081 atttataaat gatattcggg gataattatg gcgcagtcga aactctatcc agttgtgatg
    13141 gcaggtggct ccggtagccg cttatggccg ctttcccgcg tactttatcc caagcagttt
    13201 ttatgcctga aaggcgatct caccatgctg caaaccacca tctgccgcct gaacggcgtg
    13261 gagtgcgaaa gccctgtggt gatttgcaat gagcagcacc gctttattgt tgcggaacaa
    13321 ctgcgtcaac tgaacaaact cactgagaac attattctcg aaccggcagg gcgtaacaca
    13381 gcaccggcca ttgcgctggc ggcgctggcg gcaaaacgtc atagcccgga gaacgaccca
    13441 ttaatgctgg tgctggcggc ggatcatgtg attgccgatg aagacgcatt ccgcgccgcc
    13501 gtgcgtaacg ctatgccgta tgccgaagcg ggcaagctgg tgaccttcgg cattgtgccg
    13561 gatctacctg aaaccggata tggctatatt cgtcgcggtg aagtgtcggc gggtgagcag
    13621 gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac caaatctgga aaccgcgcag
    13681 gcctatgtgg caagcggcga atattactgg aacagcggta tgttcctgtt ccgtgccgga
    13741 cgctatctcg aagaactgaa aaaatatcgc ccggatattc tcgacgcctg tgaaaaagcg
    13801 atgagcgccg tcgatccgga tctcgatttt attcgcgtgg atgaagacgc gtttctggcc
    13861 tgcccggaag agtcggtgga ttacgcggtc atggaacgta cggcagatgc cgttgtggtg
    13921 ccgatggatg cgggctggag cgatgtcggc tcctggtcct cattatggga gatcagcgcc
    13981 cacaccgccg agggcaacgt ttgccacggc gatgtgatta atcacaaaac tgaaaacagc
    14041 tatgtgtatg ccgaatctgg cctggtcacc accgtcgggg tgaaagattt ggtggtagtg
    14101 cagaccaaag atgcggtgct gattgccgac cgtaacgcgg tgcaggatgt gaaaaaagtg
    14161 gtcgagcaga tcaaagccga tggtcgccat gagcatcggg tgcatcgcga agtctatcgc
    14221 ccgtggggca aatatgactc tatcgacgcg ggcgaccgct accaggtgaa acgcatcacc
    14281 gtgaaaccgg gcgaaggctt gtcggtacag atgcaccatc accgcgcgga acactgggtg
    14341 gttgtcgcgg gaacggcaaa agtcaccatt gacggtgata tcaaactgct tggtgaaaac
    14401 gagtcgattt atattccgct gggggcgacg cactgcctgg aaaacccggg gaaaattccg
    14461 ctcgatttaa ttgaagtgcg ctccggctct tatctcgaag aggatgatgt ggtgcgcttc
    14521 gcggatcgct acggacgggt gtaaacgtcg catcaggcgc tgattgtcgg atgcggcgtg
    14581 aacgccttat ccgacctacg gttcggtttt gtaggcctga taagacgcgg cagcgtcgca
    14641 tcaggcaatg aatgcgaaac cgcggtgtaa ataacgacaa aaacaaaatt ggccgtttcg
    14701 gtcagggcca actattgcct gaaaaagggt aacgatatga aaaaattaac ctgctttaaa
    14761 gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatattgc ctggcgcatt
    14821 ggtcgtgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg tgatgcccgt
    14881 ctcaccagcg aaaccttaaa actggcgctg gcaaaaggtt tacaggatgc gggcgttgac
    14941 gtgctggata ttggtatgtc cggcaccgaa gagatctatt tcgccacgtt ccatctcggc
    15001 gtggatggcg gcattgaagt tactgccagc cataacccga tggattacaa cggcatgaaa
    15061 ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg gactgcgcga tgtccagcgt
    15121 ctggcagaag ccaacgactt tcctcccgtt gatgaaacca aacgcggtcg ctatcagcaa
    15181 atcaatctgc gtgacgctta cgttgatcac ctgttcggtt atatcaacgt caaaaacctt
    15241 acgccgctca agctggtgat caactccggg aacggcgcgg cgggtccggt ggtggacgcc
    15301 atcgaagccc gctttaaagc cctcggcgca ccggtggaat tgatcaaagt gcacaacacg
    15361 cccgacggca atttccccaa cggtattcct aacccgctgc tgccggaatg tcgcgacgac
    15421 acccgcaatg cggtcattaa acacggcgcg gatatgggca ttgcttttga tggcgatttt
    15481 gaccgctgtt tcctgtttga cgaaaaaggg cagtttatcg agggctacta cattgtcggt
    15541 ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca cgatccacgt
    15601 ctctcctgga ataccgttga tgtggtgact gccgcaggcg gcaccccggt aatgtcgaaa
    15661 accggacacg cctttattaa agaacggatg cgcaaggaag acgccatcta cggtggcgaa
    15721 atgagcgccc accattactt ccgtgatttt gcttactgcg acagcggcat gatcccgtgg
    15781 ctgctggtcg ccgaactggt gtgcctgaaa ggaaagacgc tgggcgaact ggtgcgcgac
    15841 cggatggcag cgtttccgtc aagcggtgag atcaacagca aactggcgca acccgttgag
    15901 gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc tggcggtgga tcgcaccgat
    15961 ggcatcagca tgacctttgc cgactggcgc tttaatctgc gctcctccaa caccgaaccg
    16021 gtggtgcggt tgaatgtgga atcacgcggt gatgtgccgc tgatggaagc gcgaacgcga
    16081 actctgctga cgttgctgaa cgagtaatgt cggatcttcc cttaccccat tgcgggtaag
    16141 gggctaataa caggaacaac gatgacaaat ctaaaaaagc gcgagcgaac gaaaaccaat
    16201 gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt tgccggacta
    16261 tggctggttt gcgaagtgag cggactgtca ttcctctaca tgcacctgtt ggtggcgctg
    16321 attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg ctcatggcgc
    16381 ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt aagcgtgatt
    16441 ttcagcgccg gactggtggc gttcaacaat gatttcgaca cgcaactgaa aatctggctg
    16501 gcgtggtatg ggctgaccag tatcggactg gtggtttgcc gttcatgtat tcgcattggg
    16561 gcgggctggc tgcgtaatca tggctataac aagcgcatgg tcgcggtggc gggggattta
    16621 gccgccgggc aaatgctgat ggagagcttc cgtaaccagc cgtggttagg gtttgaagtg
    16681 gtgggcgttt accacgaccc gaaactgggc ggcgtttcta acgactgggc gggtaacctg
    16741 caacagctgg tcgaggatgc taaagcaggc aagattcata acgtctatat cgcgatgcaa
    16801 atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac tggcggacac cacctgttcg
    16861 gtgctgctga tccccgatgt ctttaccttc aacattctcc attcacgtct tgaagagatg
    16921 aacggcgttc cggtggtgcc gctttacgac acgccgcttt ccggggttaa ccgcctgctc
    16981 aaacgtgcgg aagacattgt gctggcgacg cttatcctgc tgctgatctc cccggtgctg
    17041 tgctgcattg cgctggcggt gaaactcagt tcacctgggc cggttatttt ccgccagact
    17101 cgctacggca tggatggcaa gccgatcaaa gtgtggaagt tccgttccat gaaagtgatg
    17161 gagaacgaca aagtggtgac ccaggcgacg cagaacgatc cgcgcgtcac caaagtgggg
    17221 aactttctgc gccgcacctc gctggatgaa ttgccgcagt ttatcaatgt gctgaccggg
    17281 gggatgtcga ttgtcggtcc acgtccgcac gcggtggcgc ataacgaaca gtatcgacag
    17341 ctcattgaag gctacatgct gcgtcataag gtgaaaccgg gcattaccgg ctgggcgcag
    17401 attaacggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg cgtcgagttc
    17461 gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt tttcctgacg
    17521 gtattcaaag gattcgttaa caaagcggca tattgatatg agcttacgtg aaaaaaccat
    17581 cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc atcggcctcg ggctggtgca
    17641 gatgaccgtg ctggcgcgga ttatcgacaa ccaccagttc ggtttgctta ccgtgtcgct
    17701 ggtgatcatc gcgctggcag atacgctttc tgacttcggt attgctaact cgattattca
    17761 gcgaaaagaa atcagccacc ttgaactcac cacgctgtac tggctgaacg ttgggctggg
    17821 gcttgtggtg tgtgtggcgg tgtttttgtt gagtgatctc attggtgacg tgctgaacaa
    17881 cccggacctg gcaccgttga ttaaaacatt gtcgctggcg tttgtggtta tcccccacgg
    17941 gcaacagttc cgcgcgttga tgcaaaagga gctggagttc aacaaaatcg gcatgatcga
    18001 aaccagcgcg gtactggcgg gcttcacttt tacggtggtt agcgcccatt tctggccgct
    18061 ggcgatgacc gcgatcctcg gttatctggt caatagtgcg gtgagaacgc tgctgtttgg
    18121 ctactttggc cgcaaaattt atcgccccgg tctgcatttc tcgctggcgt ctgttgcgcc
    18181 gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc tcaataccaa
    18241 cctttcaacg ctggtgctgg cgcgtattct cggcgcgggc gtggcagggg gatacaacct
    18301 ggcgtacaac gtggccgttg tgccgccgat gaagctgaac ccgatcatca cccgtgtgtt
    18361 gtttccggca ttcgccaaaa ttcaggacga taccgaaaag ctgcgcgtca acttctacaa
    18421 gctgctgtcg gtggtgggga ttatcaactt tccggcgctg ctcgggctga tggtggtgtc
    18481 aaataacttt gtaccgctgg tatttggcga aaagtggaac agcattattc cggtgctgca
    18541 attgctgtgt gtggtgggtc tgctgcgctc cgtaggtaac ccgattggtt cgctgctgat
    18601 ggcgaaagcg cgggtcgata tcagctttaa attcaacgta ttcaaaacat ttctgtttat
    18661 tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc tcggtttcct
    18721 gctggtgcag attatcaaca ccattctgag ttacttcgtg atgattaaac cggttcttgg
    18781 ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct cgctgccgac
    18841 gctggtggtc agttatgtgc tgggcattgt gctgaaaggg caactggcgc tggggatgct
    18901 gctggcggtg caaatagccg cgggcgtgct ggcgtttgtg gtgatgattg tgctgtcgcg
    18961 ccatccgctg gtggtggaag tgaagcgcca gttttgtcgc agcgaaaaaa tgaaaatgct
    19021 tttacgggcg gggtgagttg ttgttttcgg ttccgacaac ggttgccggg tgcagcgtaa
    19081 aacaccttat ccatcctacg gttctgtgct cgggtcttgt aggcctgata agacgcgcca
    19141 gcgtcgcatc aggcaaaaaa cggcaacgaa cacaacaaac acagcctcac ctggcatcaa
    19201 atttatgagg aaaaaatgaa attacttatt ctgggcaacc acacttgcgg caatcgtggc
    19261 gacagcgcca tcttgcgcgg cttacttgat gccatcaaca tcctcaatcc acataccgaa
    19321 gtggacgtga tgagccgcta tccggtcagt tcttcctggc tgctcaaccg cccggtaatg
    19381 ggtgatccgc tgttccttca aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc
    19441 gttaaaaaag tcctccgtcg ccgctatcag caccaggtat tgctctcacg cgtcaccgac
    19501 actggcaagc tgcgcaatat cgccatcgcc cagggattca ccgacttcgt gcgcctactg
    19561 tcaggttacg acgccattat tcaggtcggc ggatcgtttt ttgtcgatct ctacggcgtg
    19621 ccgcagtttg aacatgcact ttgcacattt atggcgaaaa agccgctgtt tatgattggt
    19681 cacagcgtcg gtcccttcca ggatgagcaa tttaaccaac tggcgaacta cgtttttggt
    19741 cactgcgacg cgctgatctt gcgcgaatcg gtcagcctcg atctaatgaa acgcagcaat
    19801 atcaccactg caaaagttga acatggcgtc gataccgcgt ggctggtcga tcaccacaca
    19861 gaagacttca ccgccagcta tgccgtccaa cactggctgg acgttgccgc acaacagaaa
    19921 acggtggcaa ttaccctgcg cgaactggca ccgttcgaca aacgcctcgg caccactcag
    19981 caagtgtatg aaaaagcctt tgccggggtg gtcaatcgca ttctcgacga aggctatcag
    20041 gtcattgcgc tctccacctg tacgggtatc gacagctata acaaagacga ccgcatggtg
    20101 gcgctcaacc tgcgccagca catcagcgat cctgcccgtt atcacgtagt gatggatgaa
    20161 cttaacgatc tggaaatggg caaaattctc ggtgcctgtg aactcaccgt cggtacgcgc
    20221 ctgcactccg ccattatctc aatgaacttt gctactccgg cgattgccat caactatgaa
    20281 cataaatccg ccggggttat gcagcagctg ggactaccgg agatggcaat tgatatccgt
    20341 catttattag acggcagcct gcaagcgatg gttgcggata ccttaggcca gcttccggcg
    20401 ctgaacgcac gacttagcga agccgtcagt cgtgagcgtc agacgggaat gcagatggtg
    20461 cagtccgtac tggaacgcat cggggaggtg aaatgaaggt cggcttcttc ttattgaaat
    20521 ttccgctgtc gtcagaaacc ttcgttctta accaaatcac tgcgtttatt gatatgggct
    20581 ttgaagtgga gattgtcgcg ctgcaaaaag gcgacacaca aaacacccac gtggcatgga
    20641 ctaaatacaa ccttgccgcc agaacccgct ggttacagga cgaacctacg ggcaaagtgg
    20701 cgaaactgcg ccaccgagcc agtcagacct tgcgcggcat tcatcgtaaa aatacctggc
    20761 aggcgctcaa cctcaaacgc tatggtgccg agtcgtggaa cctgattttg tctgccattt
    20821 gcggccaggt cgcaacaccg tttcgcgccg atgtgttcat cgctcatttt ggccctgcgg
    20881 gggtaaccgc agcaaaacta cgcgaactgg gtgtcattca cggcaaaatt gccactattt
    20941 tccacggcat tgatatttcc agtcgggaag tgctcaacca ttacactccc gaatatcaac
    21001 aactgtttcg acgtggcgac ctgatgctac cgataagcga tctgtgggcc ggaaggctgc
    21061 aaaaaatggg ctgcccgagg gaaaaaatcg ccgtatcgcg catgggcgta gatatgacgc
    21121 gctttagccc gcgtcccgtg aaagcgcccg caacaccgct ggagattatt tccgtcgcac
    21181 gcttaaccga gaaaaaaggt ctgcatgtgg cgattgaagc ctgccggcag ttgaaagagc
    21241 agggcgtggc atttcgctat cgcatcctcg gcattggccc gtgggaacga cgcctgcgca
    21301 cgctcatcga acaatatcaa ctggaagatg tggtggagat gccgggcttt aaaccgagcc
    21361 atgaagtgaa agcgatgctc gacgacgcgg atgtcttcct gttgccatcg gttacaggtg
    21421 cggatggcga tatggaaggc attccggtgg cgctgatgga ggcgatggcg gtcggcattc
    21481 cggtggtttc tactctgcat agcggaatac cggaactggt ggaggctgac aaatccggct
    21541 ggctggtgcc tgagaacgat gctcgcgcac tggcgcaacg actggcggcg tttagccaac
    21601 tggacaccga cgaattggct ccggtcgtca aacgcgcgcg cgaaaaagtt gaacacgatt
    21661 ttaaccagca ggtgatcaat cgagaactcg ccagcttgct gcaggcttta tagaggagaa
    21721 cgtatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc ggcgcttgcc
    21781 ttccttcata cccctttcgc tcgcgcactt cccgcccgac aaagcgttaa tatcaacgac
    21841 tacaaccctc acgactggat cgcctcattt aaacaagcct tcagcgaagg gcaaacggtt
    21901 gtcgtgcctg ctggattcgt ttgtgacaat atcaacaccg gcatcttcat tcctcctggc
    21961 aaaacgttac acatccttgg aagcctgcgc ggcaacggca gagggcgatt tgtcttacag
    22021 gacggcagcc aggtgacagg ggaggagggc ggcagtatgc ataacatcac cctggatgtg
    22081 cgtggttctg actgcaccat caaagggctg gcgatgagcg gctttggtcc ggtaacgcag
    22141 atttatatcg gcggcaaaaa caaacgggtc atgcgcaacc tgaccatcga taacctgacc
    22201 attagccacg ctaattacgc catcttacgc cagggatttc ataaccagat tatcggtgcc
    22261 aacatcacca attgtaagtt cagcgactta cagggcgatg ccattgaatg gaacgtggcg
    22321 attaacgacc gcgatatttt gatctccgac catgtcatcg agcgcatcaa ctgtactaac
    22381 ggcaaaatca actggggcat tggcataggc cttgcgggaa gcacttacga taataattac
    22441 ccggaagatc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc ggattgtcgg
    22501 cagttgatac atgttgaaaa tggtaaacat tttgttattc gtaatatcaa agcccgcaat
    22561 atcacgcagg atttcagtaa gaaagcaggt attgataacg ccacagtcgc tatttacggt
    22621 tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccgggat gttaatcggc
    22681 tatggggtaa ttaaaggcaa ttatctctcg attccgcaaa atttccgagt gaataatatt
    22741 caactggata acacccatct tgcttataaa ttgcgcggcc tccaaatctc tgccgggaat
    22801 gccgtctcct ttgtggcact aactaacatt gagatgaagc gtgcgtcgct ggagttacac
    22861 aacaaaccgc aacatctttt tctgcgtaat atcaaagtga tgcaggaatc ctctgttgga
    22921 cccgcattga ttatgaactt cgacatgcgc aaagacgttc gaggcgtctt tatggcgaaa
    22981 gaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgagaaagg acaaagctcc
    23041 gtcgatatcg acaggattaa tcaccatatt gttaatgtgg aaaagattaa ctttagattg
    23101 ccggaacgga gagagtagat ttgcgaccat tcataaactt actggatata atgttctaaa
    23161 agattataat cctcacacta actgttaaag ctggggctgg gcagcattga ctactcaggg
    23221 aaggtactca tctttatctt gtgtttctta cgtaacaacc agactaaagt gatttatgaa
    23281 cgataacgtt ttgctcatag gagcttccgg attcgtagga acccgactac ttgaaacggc
    23341 aattgctgac tttaatatca agaacctgga caaacagcag agccactttt atccagaaat
    23401 cacacagatt ggtgatgttc gtgatcaaca ggcactcgac caggcgttag ccggttttga
    23461 cactgttgta ctactggcag cggaacaccg cgatgacgtc agccctactt ctctctatta
    23521 tgatgtcaac gttcagggta cccgcaatgt gctggcggcc atggaaaaaa atggcgttaa
    23581 aaatatcatc tttaccagtt ccgttgctgt ttatggtttg aacaaacaca accctgacga
    23641 aaaccatcca cacgaccctt tcaaccacta cggcaaaagc aagtggcagg cggaggaagt
    23701 gctgcgtgaa tggtataaca aagcaccaac agaacgttca ttaactatca tccgtcctac
    23761 cgttatcttc ggtgaacgca accgcggtaa cgtctataac ttgctgaaac agatcgctgg
    23821 cggcaagttt atgatggtgg gcgcagggac taactataag tccatggctt atgttggaaa
    23881 cattgttgag tttatcaagt acaaactgaa gaatgttgcc gcaggttacg aggtttataa
    23941 ctacgttgat aagccagacc tgaacatgaa ccagttggtt gctgaagttg aacaaagcct
    24001 gaacaaaaag atcccttcta tgcacttgcc ttacccacta ggaatgctgg gtggatattg
    24061 ctttgatatc ctgagcaaaa ttacgggcaa aaaatacgct gtcagctctg tgcgcgtgaa
    24121 aaaattctgc gcaacaacac agtttgacgc aacgaaagtg cattcttcag gttttgtggc
    24181 accgtatacg ctgtcgcaag gtctggatcg aactctgcag tatgaattcg tccatgccaa
    24241 aaaagacgac ataacgtttg tttctgagta atgcagacga gtcagagaaa aaaggaccag
    24301 gttggactac gattattcgg aaagaggtac tgattaaatt atcagtatgt ggatttccga
    24361 gtaatccgag taacccagat gctgatggtg tgtagtgtta tcgtaaggtt taatcaaagt
    24421 tatgcggaac taagctttgg tatatagcca taacgtttgg ctaacctatg gcaataattt
    24481 acgcactatc agccacttag aggtaggaaa agaatgacga atttaaaagc agttataccg
    24541 gtagcaggtc ttgggatgca tatgttgcct gccactaagg cgattcccaa agagatgcta
    24601 ccgatcgtcg acaagccaat gattcagtac attgttgacg agattgtggc tgcagggatc
    24661 aaagaaatcc tcctggtaac tcacgcgtcc aagaacgcag tcgaaaacca cttcgacacc
    24721 tcttatgaat tagaatctct ccttgaacag cgcgtgaagc gtcaactact ggcggaagta
    24781 caatctatct gtccgccggg cgtgaccatt atgaacgtgc gtcagggtga acctttaggt
    24841 ttaggccact ccattttatg tgcgcgacct gccattggtg acaatccatt tgtcgtggtg
    24901 ctgccagacg ttgtgatcga cgacgccagc gccgacccgc tacgctacaa ccttgctgcc
    24961 atgattgcgc gcttcaatga aacgggacgc agccaggtgc tggcaaaacg tatgccgggc
    25021 gacctctctg aatactccgt catccagacc aaagaaccgc tggatcgtga aggcaaagtc
    25081 agccgcattg ttgaatttat cgaaaagccg gatcagccgc agacgctgga ctcagacatc
    25141 atggccgttg gtcgatatgt gctttctgcc gatatttggc cggaactgga acgtactcag
    25201 cctggtgcat ggggacgtat tcagctgact gatgccattg ctgagctggc aaaaaaacaa
    25261 tccgttgatg caatgctgat gactggcgac agttacgact gcggtaaaaa aatgggctat
    25321 atgcaggcgt ttgtgaagta tggactacgc aacttgaaag aaggggcgaa gttccgcaaa
    25381 ggtatcgaga agctgttaag cgaataatga aaatctgacc gaatgtaacg gttgataagg
    25441 aaataatatc ggcagtgaaa aactatagat caataaatat cactgctgtt gagcttttat
    25501 aattaactat gctaccaatg aataaatagt gtgcagatat gtattttttg tttggtagtc
    25561 ttgtttctcc ctctgaataa taagataata aacgcttgga gatgattttt ttaacatgga
    25621 ttatcatttc gtacactggt agcgattaag ccaggggcgg tagcgtgcat aaattttaat
    25681 gcttatcaaa actattagca ttaaaaatat ataagaaatt ctcaaa
//
LOCUS       DAZGXS010000064        25715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGXS010000064 DAZGXS010000000
VERSION     DAZGXS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            27..1427
                     /locus_tag="ACHKG5_002691"
     CDS             27..1427
                     /locus_tag="ACHKG5_002691"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0868772.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1583..3118
                     /gene="gadC"
                     /locus_tag="ACHKG5_002692"
     CDS             1583..3118
                     /gene="gadC"
                     /locus_tag="ACHKG5_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0868773.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3249..4568
                     /locus_tag="ACHKG5_002693"
     CDS             3249..4568
                     /locus_tag="ACHKG5_002693"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0868774.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4945..6327
                     /gene="dosC"
                     /locus_tag="ACHKG5_002694"
     CDS             4945..6327
                     /gene="dosC"
                     /locus_tag="ACHKG5_002694"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP0868775.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTVASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6352..8751
                     /gene="dosP"
                     /locus_tag="ACHKG5_002695"
                     /gene_synonym="pdeO"
     CDS             6352..8751
                     /gene="dosP"
                     /locus_tag="ACHKG5_002695"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0868776.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDNPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            9009..9590
                     /gene="ddpX"
                     /locus_tag="ACHKG5_002696"
     CDS             9009..9590
                     /gene="ddpX"
                     /locus_tag="ACHKG5_002696"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0868777.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQSNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            9604..11154
                     /locus_tag="ACHKG5_002697"
     CDS             9604..11154
                     /locus_tag="ACHKG5_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0868778.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            11156..12179
                     /locus_tag="ACHKG5_002698"
                     /pseudo
     CDS             11156..12179
                     /locus_tag="ACHKG5_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            12176..13072
                     /gene="ddpC"
                     /locus_tag="ACHKG5_002699"
     CDS             12176..13072
                     /gene="ddpC"
                     /locus_tag="ACHKG5_002699"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0868779.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            13069..14055
                     /locus_tag="ACHKG5_002700"
     CDS             13069..14055
                     /locus_tag="ACHKG5_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0868780.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            14048..14974
                     /locus_tag="ACHKG5_002701"
     CDS             14048..14974
                     /locus_tag="ACHKG5_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0868781.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(15030..15461)
                     /gene="osmC"
                     /locus_tag="ACHKG5_002702"
     CDS             complement(15030..15461)
                     /gene="osmC"
                     /locus_tag="ACHKG5_002702"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0868782.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15806..16021
                     /gene="bdm"
                     /locus_tag="ACHKG5_002703"
     CDS             15806..16021
                     /gene="bdm"
                     /locus_tag="ACHKG5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0868783.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            16123..16260
                     /gene="sra"
                     /locus_tag="ACHKG5_002704"
     CDS             16123..16260
                     /gene="sra"
                     /locus_tag="ACHKG5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0868784.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            16417..18114
                     /gene="maeA"
                     /locus_tag="ACHKG5_002705"
     CDS             16417..18114
                     /gene="maeA"
                     /locus_tag="ACHKG5_002705"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0868785.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            18248..19258
                     /gene="adhP"
                     /locus_tag="ACHKG5_002706"
     CDS             18248..19258
                     /gene="adhP"
                     /locus_tag="ACHKG5_002706"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0868786.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            19404..19688
                     /locus_tag="ACHKG5_002707"
     CDS             19404..19688
                     /locus_tag="ACHKG5_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0868787.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(19916..20569)
                     /gene="fdnI"
                     /locus_tag="ACHKG5_002708"
     CDS             complement(19916..20569)
                     /gene="fdnI"
                     /locus_tag="ACHKG5_002708"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0868788.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20562..21446)
                     /gene="fdnH"
                     /locus_tag="ACHKG5_002709"
     CDS             complement(20562..21446)
                     /gene="fdnH"
                     /locus_tag="ACHKG5_002709"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0868789.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(21459..24506)
                     /gene="fdnG"
                     /locus_tag="ACHKG5_002710"
     CDS             complement(21459..24506)
                     /gene="fdnG"
                     /locus_tag="ACHKG5_002710"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23919..23921),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0868790.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24738..25619
                     /gene="yddG"
                     /locus_tag="ACHKG5_002711"
     CDS             24738..25619
                     /gene="yddG"
                     /locus_tag="ACHKG5_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0868791.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6276 a         6555 c         6532 g         6352 t
ORIGIN      
        1 gatccaatca ttttaaggag tttaaaatgg ataagaagca agtaacggat ttaaggtcgg
       61 aactactcga ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa tcaaaacgtt
      121 ttccgctgca cgaaatgcgc gacgatgtcg catttcagat tatcaatgac gaattatatc
      181 ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac gacgaaaacg
      241 tccacaagtt gatggattta tccattaaca aaaactggat cgacaaagaa gaatatccgc
      301 aatctgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
      361 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
      421 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
      481 cggataaacc aaacctggtg tgcggcccgg tgcaaatctg ctggcataaa ttcgcccgct
      541 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggatccga
      601 aacgcatgat tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga
      661 cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcgctggat aaattccagg
      721 ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagtggtggc ttcctggcac
      781 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
      841 cttcaggcca caaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgatg
      901 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt cagattggta
      961 ctttcgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     1021 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     1081 atctggcgga tgaaatcgcc aaactggggc cgtatgagtt catctgtacc ggtcgcccgg
     1141 acgaaggcat tccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     1201 tgtatgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     1261 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     1321 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agtgatcacc
     1381 cgaaactgca gggtattgcc caacagaaca gctttaaaca tacctgataa cgtttaacgg
     1441 taacggtgtc ccgaaacgaa cccgtttcgg gacaatttcc aaagtctgtt cactggcatt
     1501 agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg
     1561 aataccgttt tagggggata atatggctac attagtacag acaggtaaag ctaagcagct
     1621 cacattactc ggcttttttg ccataacggc atcgatggta atggctgttt atgaataccc
     1681 taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg ggattttatg
     1741 gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct gggaagaagg
     1801 tggtgtcttc gcctgggtat caaatactct ggggccgaga tggggatttg cagcgatctc
     1861 atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg tgttaggggc
     1921 actctcctac atcctgaaat ggccagcgct gaatgaagac cccattacca aaactattgc
     1981 agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga aatacacggc
     2041 gcgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta ttttgattgc
     2101 attagcggct atttatctgc actccggtgc ccccgttgct atcgaaatgg attcgaagac
     2161 cttcttccct gacttctcta aagtgggcac cctggttgta tttgttgcct tcattttgag
     2221 ttatatgggc gtagaagctt ccgcaaccca cgtcaatgaa atgagtaacc cagggcgcga
     2281 ctatccactg gctatgttac tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg
     2341 tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca
     2401 aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg ttcgcgtgat
     2461 ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg ttggtccttc
     2521 tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa
     2581 caaaaatggc gtaccggtaa cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt
     2641 gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg cactggcgct
     2701 gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca ttgtgttggt
     2761 tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag gggtgaaact
     2821 ggtcgtggca attgtcggtc tgctgacttc aattatggcg tttattgttt ccttcctgcc
     2881 gccggataac atccagggtg actctaccga tatgtatgtt gaattactgg tcgttagttt
     2941 cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta aaggcaaagc
     3001 gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag gtcacttctt
     3061 cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga aacactaact
     3121 atgtctgaat ggttataaga taagggagcg ataattcatc gctccctttt tcgtgcttgc
     3181 tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt taccggaaac
     3241 tagcacatat ggatatctgc tcccgaaaca agaaattaac gattagaaga ccagcgatac
     3301 tggttgcact ggcactttta ctgtgtagtt gtaaaagcac gcctccagag tccatggtga
     3361 caccaccagc gggttcaaag ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg
     3421 gcatctggct ggccacggtg tctcgcctcg actggccacc ggtttcctcg gttaacatta
     3481 gtaaccccac cagccgggcc cgtgtacaac aacaggcgat gatcgacaaa ctggatcatt
     3541 tgcaacgtct cggtataaac acggtctttt tccaggtcaa gccggacggt accgccctgt
     3601 ggccatcgaa aattttgccg tggtccgatc ttatgaccgg taagattggt gaaaatccgg
     3661 gttacgatcc gctgcaattt atgctcgacg aagcccacaa gcgtgggatg aaagtacacg
     3721 cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc agggaactga
     3781 atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac tggatcagaa
     3841 cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac tggatcacat
     3901 caatagtcgc agaagtggtt tcccgctatc cggtagatgg cgtgcagttt gacgactatt
     3961 tctatactga gtcaccgggt tcacggctaa atgataacga aacgtaccgt aaatacggag
     4021 gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta attgcaaagg
     4081 tatcgcacac cattaaaagc attaagccgg gagtcgaatt tggtgttagc ccggcaggcg
     4141 tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg gcagcctatg
     4201 acgaatccta cgctgacact cgtcgatggg tggaacaagg attgctggat tacattgctc
     4261 cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt
     4321 gggcggatgt cgttaaaccg accaggaccc gcctgtatat cggtatcgcc ttctataaag
     4381 tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta ccggagctga
     4441 aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc ttgttccgtg
     4501 aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa agtcgttggg
     4561 gcagttaaac tcccattctt actttgtaac aagccggtgc tttacccact ggcttgttac
     4621 cctcttgaac tatcccacca gcaatcatgc ttactaaagt caaattaacc acgcaattaa
     4681 ccacgcttag ttaaacagcc ataaatcccc taataaaaat gtatgaatat gaaaaaccat
     4741 acgatattcg cagtcaccga aaaataatta cgatgggcta tactcttctg aagaacagta
     4801 gcgtagattg aaactgtcct aagtcgctta tttcttttca gtatatcttc atatttcagg
     4861 agaatatggt gcagttagtc gatctcgcac gtcgcgtttc attttgattc cgtctgattt
     4921 cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa agatgagtgg
     4981 accggacttg tcgagcaggc agatccgccc attcgtgcta aagccgcgga aattgccgtt
     5041 gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga cccgcatgcc
     5101 gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat ggaacgctgg
     5161 attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca aatccagcat
     5221 accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga gatggggttt
     5281 cgggtgctga aaaagatcct ctatccggta atcttctctt cggattattc tgccgcagaa
     5341 aaacttcagg tctaccattt ctcgattaac agtattgata tcgctatgga agtgatgacc
     5401 cgcgcgttta cctttagtga cagtagtgcc tcaaaagaag atgaaaacta tcgtatcttc
     5461 tcgttactgg aaaacgccga agaagaaaaa gaacggcaaa tagcctcaat actttcatgg
     5521 gaaatagata ttatctataa aatcctgctg gattctgatt taggcagtag tttgccttta
     5581 agccaggctg attttggcct gtggtttaac cataaaggtc gacattattt cagcggcatt
     5641 gctgaagtgg gccatatctc tcgtctgatt caggatttcg acggtatttt caatcaaacc
     5701 atgcgtaaca ccaggaattt gaataacaga agtctgcggg tgaaattttt attacagata
     5761 agaaataccg tatcgcaaat tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc
     5821 cacgaagtcg gtatggatgt actgacgaaa ttacttaacc gccgtttcct gccgactatc
     5881 ttcaaacgcg aaattgccca tgccaaccgg accggtacac cgctgtcagt gctgattatt
     5941 gacgttgata aattcaaaga gatcaacgat acgtggggcc ataacactgg cgatgaaatt
     6001 ctgcgtaaag tctctcaggc cttttatgac aacgtccgca gtagtgatta tgttttccgc
     6061 tacgggggcg atgaatttat cattgttttg actgtagctt ctgaaaacga aacgttacgt
     6121 accgcagaac gtattcgcag tcgggtggag aaaaccaaac tgaaagccgc aaacggcgaa
     6181 gatattgccc tctcactttc catcggtgcc gccatgttta atggtcatcc tgactatgag
     6241 cgcctcattc aaatagccga tgaagctctg tatatcgcca aaagacgagg tagaaaccgt
     6301 gttgaactct ggaaagccag tctttagatg cgccaggatg cagaggtaat catgaagcta
     6361 accgatgcgg ataatgccgc cgatggcatt tttttccccg cccttgagca aaatatgatg
     6421 ggtgcggtgt taattaacga aaatgatgaa gtgatgtttt tcaaccccgc cgcagagaag
     6481 ctctgggggt acaaacgtga agaagtcatt ggcaataaca ttgatatgct gattccgcgg
     6541 gatttgcgtc ctgcgcatcc tgaatacatt cgtcacaacc gtgaaggcgg taaagcgcgc
     6601 gtcgagggga tgagtcggga gctgcagctg gagaaaaaag acggcagtaa aatctggacc
     6661 cgttttgcgc tatcgaaagt gagcgccgag gggaaagttt attacctggc gctggtacgg
     6721 gatgccagcg tagaaatggc gcaaaaagaa cagaccaggc aattgattat tgccgttgac
     6781 catctcgacc gaccggtgat tgtcctcgat ccggaacgcc atattgtgca gtgcaatcgc
     6841 gcatttaccg aaatgtttgg ttactgcatt agcgaagcca gcggtatgca gcctgataca
     6901 ctcctgaaca ttcctgaatt ccctgccgat aaccgcattc gtttacaaca gttgctttgg
     6961 aaaaccgccc gcgatcagga cgaatttctg ctgttgacgc gcaccggtga aaaaatctgg
     7021 attaaagctt ctatcagccc ggtttatgac gtgctcgcgc atctgcagaa cctggtaatg
     7081 accttctcgg atatcaccga agaacggcaa atccgccagc ttgaaggcaa tattctcgcc
     7141 gccatgtgca gcagcccgcc atttcatgaa atgggggaaa tcatttgtcg taacatcgaa
     7201 tctgttctca acgaatcgca tgtttcgctg ttcgcactgc gcaacgggat gccgatacac
     7261 tgggcgtcat cttcgcacgg tgcagaagtt caaaatgcgc aaagctggtc agcgaccatt
     7321 cgtcagcgtg atggcgcgcc tgcggggatc ctgcaaatta aaacctcgtc aggagcagaa
     7381 accagcgcct ttatcgaacg cgtggcagat atcagccagc atatggccgc gctggcgctg
     7441 gaacaggaaa aaagccgtca gcatattgaa caactcatcc aatttgatcc gatgaccggt
     7501 ctgccaaatc gcaataacct gcacaattac ctcgatgacc tggtcgacaa agccgtctct
     7561 ccagtggtgt atctcatcgg tgttgaccat attcaggatg tgattgatag ccttggctat
     7621 gcgtgggccg atcaggcatt gctggaagtg gtcaatcgct ttcgtgaaaa actcaaaccg
     7681 gatcagtatc tctgtcgtat cgaaggtacg cagtttgtcc tcgtgagcct ggaaaacgac
     7741 gtcagtaaca ttacccagat cgccgatgag ctacggaatg tggtcagcaa gccgataatg
     7801 attgacgata accccttccc gcttaccttg agtattggca tcagctacga cgtgggtaaa
     7861 aatcgcgatt acctgctctc cactgcccat aacgcgatgg attatattcg caagaatggc
     7921 ggtaacggct ggcagttctt cagcccggcg atgaacgaaa tggtaaaaga gcgtttggtt
     7981 ttaggcgcag cgctgaaaga agcgattagc aataaccaac tgaaactggt ttaccagccg
     8041 caaatcttcg cagaaacggg tgaactctac ggcatcgaag cccttgctcg ctggcacgat
     8101 ccccagcatg gtcatgtgcc cccttcacgg tttattcctc tcgcagaaga gattggtgaa
     8161 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     8221 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     8281 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     8341 ctgacggtag aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     8401 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     8461 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagtttt
     8521 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     8581 gggcaaagcc tcaatttaac cgtcgtggcg gaaggcgtcg aaaccaaaga gcaatttgag
     8641 atgctacgaa agatccactg tcgcgttatt cagggatatt tcttttcccg ccccctaccc
     8701 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     8761 ctctcccgca ctcgcgggtt ttcatttaac gtgacactgt cacttaaata ctgtgatttc
     8821 agctacgatt ccgggagatt ccttccttaa cttcccctct tgcaccaaaa ctgcactacg
     8881 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     8941 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     9001 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     9061 agatcgaatt gaaatacgcc tgcgctgata acatcacagg taaagctatt taccagcaag
     9121 cgcgttgtct gttacacaag gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     9181 agctatcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     9241 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     9301 acagccgtgg cacggcgatc gacctgacgc ttcgtgatga gcacgggaac atcctggata
     9361 tgggcgcagg gttcgatgaa atgcacgagc gctcccatgc ctatcaccct tccgtcccgc
     9421 ccgccgctca gagcaatcgc ctgttgctga atgcgataat gactggtggc ggttttgtcg
     9481 gtatctccag cgaatggtgg cacttcgaat taccccaggc agcgagttac cctctgctcg
     9541 ctgatcaatt cacctgtttt atatcccctg gcacacagca cgtcagttaa tcaggaaact
     9601 gccatgaaga gatcgatatc gtttcgtccc acattgctcg cgctcgtcct tgccacaact
     9661 ttcccggttg cgcacgccgc cgtaccgaaa gatatgctgg tgattggtaa ggccgccgat
     9721 ccacaaaccc tcgacccggc ggtaacaata gataataacg actggacagt gacctacccg
     9781 tcttatcagc gactggttca gtacaaaacg gacggtgata aaggctcaac cgacgttgaa
     9841 ggcgatctgg caagtagctg gaaagcgtct aacgatcaaa aagagtggac gttcaccctg
     9901 aaagataatg ctaaatttgc cgatggcaca cctgtcactg ccgaagcagt aaaactctct
     9961 tttgagcggt tactaaaaat cggccagggg ccagcagaag catttcccaa agatttaaag
    10021 attgatgctc ccgacgaaca tacggtgaag tttaccctta gccagccatt tgcaccgttc
    10081 ctctacacgc tggcgaatga cggtgcatcc attatcaatc cggcggtatt aaaggaacat
    10141 gcggcggatg atgcccgcgg cttcctcgcg caaaataccg ccggttccgg accatttatg
    10201 ctgaaaagct ggcaaaaagg tcagcaatta gttctggtgc caaatccgca ttaccccggc
    10261 aataaaccga actttaagcg agtatcggta aaaattatcg gtgaaagtgc ctcccgtcgc
    10321 ctgcagctct cccgtggcga cattgacatt gccgatgcgc tgccggtgga tcaactcaac
    10381 gccctgaagc aggaaaacaa agtcaatgtg gcagagtatc cgtcactgcg cgtcacctat
    10441 ctgtatctga ataacagcaa agcgccactt aatcaggcgg atctgcggcg ggccatttcc
    10501 tggtctaccg attatcaggg catggttaac ggcattctga gtggtaacgg aaaacaaatg
    10561 cgcggcccga ttccggaagg catgtggggc tacgatgcga cggcaatgca atacaaccat
    10621 gacgaaacga aagccaaagc cgaatgggat aaagtgacga gcaaacccac cagcctgacg
    10681 tttctctact ccgataacga tccgaactgg gaaccgatcg ctctggcaac acaatccagc
    10741 ctcaacaagc tgggcatcaa tgtgaagctg gaaaagctgg cgaacgccac catgcgcgac
    10801 agagtgggta aaggtgatta cgacattgcg attggcaact ggagtccgga ttttgccgac
    10861 ccgtatatgt ttatgaatta ctggtttgag tcagacaaaa aaggtctgcc gggtaaccgc
    10921 tcgttctatg aaaacagtga ggtcgataag ttactgcgca atgcgcttgc gaccaccgac
    10981 cagacgcagc gtacccgaga ctaccagcag gcacagaaaa tcgtcattga tgacgctgct
    11041 tatgtgtacc tgttccagaa aaactaccaa ctggcgatga acaaagaggt gaaaggcttt
    11101 gtgttcaatc ccatgctgga acaggtcttc aatatcaata ccatgagtaa ataacgtgac
    11161 cttctggagt attttacgcc aacgctgctg ggggctggtg ctcgtggtgg cgggcgtctg
    11221 cgtgattacg tttattatct cgcatctgat ccccggcgat ccggcgcggt tactggcggg
    11281 tgaccgcgcc agcgatgcta tcgtggaaaa tattcgccag caactgggac tggaccagcc
    11341 actgtatgta cagttttacc gctacgtcag cgacctgttt catggtgacc tgggaacatc
    11401 cattcgtact gggcgtccgg tgctggaaga gttgcgtata tttttcccgg cgacgctgga
    11461 gctggctttt tgtgccctgc tgctggcact cttgattggc atcccgctgg gcatactctc
    11521 tgcagtctgg cgaaatcgct ggctggatca tctggtgcga ataatggcga ttaccggaat
    11581 ctccacacct gcgttctggc ttgggctggg cgtcatcgtg ctgtttttat ggtcatctgc
    11641 aaatacttcc cggcggcgga aggcttgatg actggctgga tccaccaacg cacgttaccg
    11701 gcttttatct gctcgatgcg ttgcttgaag gcaacggtga agtcttcttc aatgcgttgc
    11761 aacatctcat cttaccggca ttaacgctgg cgtttgttca cctggggatt gtcgcccgcc
    11821 agatccgctc agcgatgctg gaacaattga gtgaagacta cattcgtacc gcccgggcca
    11881 gcggcttgcc cggctggtat atcgttctat gttatgcgct acccaatgcg ttgatccctt
    11941 cgattaccgt attggggctg gcgctgggcg atttgttgta tggcgcagtg ctcaccgaaa
    12001 ccgtttttgc ctggcccgga atgggggcat gggtagtgac atcaatacag gcgctcgact
    12061 tcccggcagt gatgggcttt gccgtcgtgg tttcatttgc ttatgtgctg gtcaacctgg
    12121 tggtggattt gctctatttg tggattgatc cgcgtatcgg acgtggaggt ggtgaatgat
    12181 gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa acgcgattta acggtgcaaa
    12241 actggtatgg atgctgaaag gcagtccgct caccgtgacc ggcgcagtca tcattgtatt
    12301 aatgctactg atgatgattt tttcaccgtg gctggcgacg catgatccca acgccattga
    12361 tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg tttggcaccg atgaagtggg
    12421 acgcgatctg ttcagccgcg tactggtcgg cagtcagcaa tcaattctcg ccggattagt
    12481 cgtggttgcc attgcgggta tgattggttc gctactcgga tgtctatccg gtgtgcttgg
    12541 cggacgcgca gacgccatca tcatgcgcat catggacatt atgctgtcga ttccttcgtt
    12601 ggtactgaca atggcactgg ctgccgctct cgggccgagt ttgtttaacg ccatgctggc
    12661 tattgctatt gtgcgaattc ccttttatgt gcgcctggcg cgggggcaag cattagttgt
    12721 acgccagtat acctatgttc aggcggcgaa aacctttggc gcgtctcgtt ggcatctgat
    12781 caactggcat attttacgta actccctacc gccgctgatc gtgcaggcat cgctggatat
    12841 cggtagcgcg attttaatgg ccgccacgtt gggatttatt ggcctgggtg ctcaacaacc
    12901 gagtgctgaa tggggcgcga tggtagcaaa tggtcgcaac tatgtactcg atcagtggtg
    12961 gtattgcgca tttccggggg cagcgatttt gcttaccgcc gtcgggttta atctctttgg
    13021 tgatggtatt cgcgatctgc ttgacccgaa agcaggagga aagcagtcat gacccaaccc
    13081 gttctggaca ttcaacaact gcatttgagt ttccccggtt ttaacggcga cgttcacgcg
    13141 ctcaacaatg tgtccttgca gattaaccgc ggtgaaattg tcggtctggt gggagaatcc
    13201 ggctcaggta aatcagtcac cgcaatgctg attatgcgtc tgctaccgac gggcagttat
    13261 tgcgtacatc gggggcagat ttcactgctg ggagacgatg ttcttaacgc gcgggaaaaa
    13321 cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc
    13381 ctcaatccga cacgtcgaat aggtcttcag atgatggacg tgatccgcca tcatcaacca
    13441 ataagtcgtc gggaagccag agctaaagcg attgccctgc tggaagagat gcaaatcccg
    13501 gataccgtgg aagttatgtc gcgctatccg tttgagcttt caggtggtat gcgccagcgg
    13561 gtaatgattg cgctggcatt ctcctgcgaa ccgcaattga ttattgccga cgaaccgact
    13621 acggcgctgg acgtcacggt acagttgcag gtactgcgtt tgcttaaaca taaagctcgc
    13681 gacagtggaa ctgcggtact gttcatcagc catgatatgg ccgtggtgtc gcaactgtgc
    13741 gatagcgttt acgtgatgta tgccggaagc gtgattgaga gcggcgtgac ggcagacgtt
    13801 atccatcatc cccggcatcc gtataccatt ggtttgctgc aatgcgcacc agaacatgga
    13861 gtaccacgcc agccattacc cgccattcca gggacggtac caaatctcac ccatttgcct
    13921 gacggctgcg cttttcgcga tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg
    13981 gcgctgacag cgtgtggtga caacaaccag cgctgcgcct gttggtatcc gcagcaggag
    14041 gtcataagtg tctgacacgt tattaacgtt acgcgacgtc catatcaatt tcccggcgcg
    14101 taaaaactgg cttggtaaaa ctacggaaca tgttcatgcc attaatggta ttgatttaca
    14161 gatccgccgt ggtgaaacct tagggatcgt cggcgagtca ggctgcggca aaagcaccct
    14221 cgcacagctt ttaatgggta tgctgcaacc gagccacggg cagtacatcc gttctggctc
    14281 acaacgcatt atgcagatga tgtttcagga cccgctctct tcgcttaatc cgcgcttacc
    14341 ggtgtggcgc atcatcacag aaccgctctg gatagctaag catagtagtg aacaacagcg
    14401 gcgagcgttg gcagaggagc tggctgtgca ggtgggtatt cgtccggagt atctcgaccg
    14461 cctgcctcat gcgttctccg gcgggcagcg gcaacgcatc gccattgcca gagcactctc
    14521 ttcgcagcct gacgtgattg tgcttgatga gccaacctct gcgctggata tctccgtgca
    14581 ggcgcagatc ctcaatttac tggtaacgct acaggaaaat cacgggctga cttatgtgct
    14641 gatttcacac aatgtctcgg tgatacgtca tatgagcgat cgggtggcgg tgatgtatct
    14701 cgggcagatt gtggaattag gggaagcgca acaggtgcta acggctccgg cacatccata
    14761 cacccgatta ttgctggatt ccctccccgc cattgataaa ccactggagg aagaatgggc
    14821 attacgtaaa acggatctgc caggaaaccg cacgttgcca cagggctgtt ttttccgcga
    14881 acgttgcccc cttgcaacca gcggatgcga agtccggcaa tcattaacga cgcgggctga
    14941 cggacgtgag atccgttgct ggcgggcgct gtagagatgc taacaacgca tcaggcattc
    15001 atgcgcctga cgcgtcatcc ggcaatgctt tacgatttca actggtaatc cagcgtaatt
    15061 tccgctttca gtacctgaga gaccgggcat cctgcttttg ctttctggat gatgccgtca
    15121 aaggtagagg catcaatacc cggcaccgca acttcactct tcagtgcgat tttcgtaatc
    15181 gcaaaaccgg catccacttt atccagcgac acatcggcgg tggtatcaat cgatgttggc
    15241 gtgaatcccg cttcccccag cattaatgaa agcgccattg agaaacatgc ggcatgcgct
    15301 gcgccaatca gttcttcagg gttggttcct ttttcgcctt caaaacgcgt gttaaatcca
    15361 tacggctgtt ggttcagcac gccactctcg gtggatactg ttcccttccc gcgtttgata
    15421 tcgctttccc agtgtgcctg acctttctta tggattgtca ttgttgctct cctgtgggct
    15481 taaaaaggaa attatagact acgtgagaaa ccgcacgagg ataagcagga tattccgaat
    15541 aaaatacggc aaaaatatta cttctgcact taaattaaat tcccacaata gataaccgga
    15601 aatatatatc tcaagagaga ttgcatcaat attaatcaac tcatcacaat aataatacct
    15661 atcaatatca tggggttaaa atataaaaat catcaaccag gactaatctt aacaacgaag
    15721 cggcaaatat ttgccatgct gaatgtgctt tataaatctg cgatccgtag cagacactat
    15781 aaatacacag acacggagaa tcactatgtt tacttattat caggcagaaa attcaacagc
    15841 agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcacg gtgttgtcac
    15901 tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata acgacatcct
    15961 cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc ctacgcttta
    16021 agatgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc ttcgctacgg
    16081 cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag
    16141 gcacgtcata ttcttggact ggaccataaa atatctaacc agcgcaaaat agttaccgaa
    16201 ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc tgaaaagtaa
    16261 ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat ggtgtttttg
    16321 tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga cgataaaagc
    16381 cccccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag
    16441 cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt gttgaataaa
    16501 ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt actgccggaa
    16561 gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca gggattcaaa
    16621 accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga aaccctcttc
    16681 taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac cccaaccgtc
    16741 ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt gtttatctct
    16801 taccagaacc gccacaatat ggacgatatt ctgcaaaacg tgccgaacca taatattaaa
    16861 gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg catcggcggg
    16921 atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat cagcccggcg
    16981 tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagctgct taacgatccg
    17041 ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga attcgttgat
    17101 gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt
    17161 gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg ttcctttaac
    17221 gatgacattc agggcaccgc ggcggtaaca gtcggcaccc tgatcgcagc aagccgcgcg
    17281 gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc
    17341 ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga ggaagcggcg
    17401 cggcagaaag tctttatggt cgatcgcttt ggcctgctga ccgacaagat gccgaacctg
    17461 ctgcctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgacag ggataccgac
    17521 agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat tctgatcggc
    17581 gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca taaacactgt
    17641 ccgcgtccga tcgtgatgcc gctgtccaac ccgacgtcac gcgtggaagc cacaccgcag
    17701 gacattatcg cctggactga aggtaacgcg ctggtcgcca cgggcagtcc gtttaatcca
    17761 gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt tattttcccg
    17821 ggcatcgggc tgggtgttat tgcttccggc gcgtcacgta tcaccgatga gatgctgatg
    17881 tcggcaagtg aaacgcttgc ccagtattcg ccgctggtcc tgaacggcga aggtctggta
    17941 ctaccggaac taaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa
    18001 atggcgcagc agcaaggcgt ggcggtgaaa acgtctgccg aagctttgca acaagccatt
    18061 gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat ctaagcctgc
    18121 gcccggtagt gaaggctacc gggctatttc cctctccctt tttcagatct catccatact
    18181 gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca tccgaaaagg
    18241 aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta cggataaaac
    18301 actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca
    18361 taccgatctt catgttaaga atggcgattt tggtgacaaa accggcgtaa ttctgggcca
    18421 tgaagggatt ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa aaccaggcga
    18481 tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact gtaacagtgg
    18541 taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg gcgggatggc
    18601 ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc
    18661 ggcggccagc agcattacct gtgcgggggt caccacctac aaagccgtta agctgtcaaa
    18721 aattcgtccc gggcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgccct
    18781 gcaatacgcg aagaatgtct ttaacgcgaa agtgatcgcc attgatgtca atgatgagca
    18841 gttaaaactg gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc
    18901 cgccaaaatt gtgcaggaga aaaccggtgg tgctcacgct gcggtggtaa cagcagtagc
    18961 taaagctgcg tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt
    19021 cggtctgccg ccggagtcta tgagcctgga tatcccacgt cttgtgctgg atggcattga
    19081 ggtggtcggt tcgctggtcg gcacgcgcca ggatctaact gaagccttcc agtttgccgc
    19141 cgaaggtaaa gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt
    19201 taccgagatg gaagaaggca aaatccgtgg ccgtatggtg attgatttcc gccgctaaga
    19261 ggcctttgct gcgactccca tgttcgggtc gcagcatcgc acactctcca acatgaaatg
    19321 gctaaatgga ttattgttgc gtaggaagga acaataatga tagtgttccc agagaaggaa
    19381 cgctactgag agtaaagaca ctaatgaaaa tggccaatca tccccgcccg ggggacatta
    19441 ttcaggaatc actggacgaa cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa
    19501 ttgctccctc aacggcaagc cgattgctga ccggaaaagc agctttgacg ccagaaatgg
    19561 caattaaact ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg
    19621 cctggagtct ggcagaggct gcaaaaacgg tggatgtgtc gcgattacgc cgtctggttg
    19681 cgcaataaaa aatggcgcct tttacagcgc catttgaaac tgatgacaaa cgcaacattg
    19741 cctgatgcgc tacgcttatc aggcctacac gttcactgca atctattgga tttgaacggt
    19801 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagccta cgttgtctac
    19861 aatatgaaaa tggcgccttt tacagcgcca ttttcagctt aaagttcaaa agagattata
    19921 tcccttcttc actctctttt ttcgcttctg ccttctcgat ttcacgatac cagcgcggat
    19981 ggtgtttctt cgcccagcga cggctcacct tcccttcgat catcccttta atcgatcctt
    20041 tcacccagaa tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag
    20101 ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg
    20161 gacgccagat aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga
    20221 acatcatctt ttgcccggcg ttgtacttac cgacatccgc cactttatgc tcattgcctt
    20281 tcaatacttc gacaatgttt aacagccacg gaatatcttt cttatccggg atgttgtgat
    20341 gcacaaaacg cacaaacata aacatcagtg cgacgaaaat cgcaatgccg aagaatgggt
    20401 gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga
    20461 agaagaacga aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac
    20521 aggcgcgatc aataaatttg gtgcgcacaa tcattttcga cttactcatg atgatcctcc
    20581 tcgtcatcgt ccacttcctt attcgggcca ataccgatgt agtggaaaat caaccctgca
    20641 aaagtggcaa taaagccagc cgctgccagc ggtttcaacg cgcctttcca caggcttacc
    20701 gaggtgtcga tcttcggatc tttcggcaga ccgtgataca gctccggctg atcggcgtga
    20761 tgcagcacgt acataacgtg cgtaccaccg accccttccg ggttgtagac gccagcatgt
    20821 tcgtaaccac gcgctttcag tttcgccacg cgctgttccg ccagctccag catctccttc
    20881 ttggtgccga agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg
    20941 acgctgacgc gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg
    21001 cgcggaatat taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac
    21061 tggaaatcga caatcccgtt agcgtactga atgattgcac cggcagacgg gcacgccttc
    21121 aggcagcccg gatcttcaca gtgcatacag ccatctttac ggatcagcca ctccagcttg
    21181 ccgctctgtt cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg
    21241 gggttatcgt aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa
    21301 cacgccacct gacaggcttt acagccgata caggtggaaa cgtcgataag ttttgcgact
    21361 tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg
    21421 ataatgtcct gcgtttccat agccatctgt tcgccccctt acgccttctc gatgttgact
    21481 aagaacgctt tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg
    21541 ttagctatat aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc
    21601 gtttcaacct gctgaccatt tacattcagc ggtttcagac gacgcgttac cacagccacc
    21661 gcgcggataa agccacgctt gcttgagaca gtgacacgat cgccattatt aatgcctttc
    21721 gccgccgcca gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc
    21781 aacgcgtgct tggtccaggt gtggaagtgc tcggtcagac gataggtcgt acccacatac
    21841 gggaactgct cttttttacc catccgcagc gcgtcttgtt catacagacg aaccaccggg
    21901 ttagaaacca cgttcggatg cagcgggtta gttcccagcg gcgtttcaat tggctcgtag
    21961 tgttccggga acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc
    22021 ggctgcatga taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtcagggata
    22081 tcgttacccg tccacttgct gccgttccac tggatcagca tccgtttcgg atcccacggt
    22141 ttaccgttga tatctgccga agcacggttg tagagtacgc gacggttaag cggccacgcc
    22201 caggcccatc ccagcgtatt ccccagaccg gacgggtcgg agttatcgcg gttagccatc
    22261 tggttgccct gctctgtcca gctaccggtg tagatccagc aagaagatgc ggttgtaccg
    22321 tcatcacgca gatgcgcaaa gctactcagc aactgacctt tcttcgcaat cagcacgcca
    22381 ttagcatcat agagatcttc cagcgcatag ccgttgttct ctttagctac ttcgtcagat
    22441 tgcggttcgt gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta
    22501 ccaccttcag cctggtacag ctcgcgcaga tgatggtaga tacccgccag aatttcgccg
    22561 tcgttacgcg cttcgcccgg cgcgtcctga cctttccagt gccactgcaa ccagcgaccg
    22621 gagttagcga tagaaccatc ttcttctgca aagcaggtcg aaggcagacg gaatacttca
    22681 gtctgaatag acgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggtagag
    22741 gtttcagtca ccagcggatc gataaccacc atgtacttca gcttgctcag gcagctcacc
    22801 actttgtttt tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa ataaccggtg
    22861 actttgcctt catccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc
    22921 ggcagccagt catagcccca gttgttctct ttctgcgcgg catcgccata gaaagatttc
    22981 atcaggctaa cgaagaactt cggatagttg ctccagtagt tcacctgacc agccagcgtc
    23041 gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggcagc
    23101 gtcagataac ctggcaggct ggtagagagc agacctaagt cagtcagacc ctgaatgttg
    23161 gagtgaccac gcaatgcatt cacgccgcca ccggccatac ccatgttacc gagcagcaac
    23221 tggatcatcg ccatagtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc
    23281 gcgtacagga aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact
    23341 ttcaggaagt cggcttttgg cgtaccacag atgttttcaa caacgtccgg cgtgtagcgg
    23401 gaaacgtgct ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt
    23461 tttgcatagc cgttttcatc gaactgatag ttccaggacg acttatcgta ctggcgtttt
    23521 tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa aatcatcacg caccagcagg
    23581 ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt tgttgttttc gatcaggtag
    23641 cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac gaataggcgc gtagatatcc
    23701 gccacagaag cggtacgcgt aaaacgggga tcgacaacga tcaaggttgc atcgttgttg
    23761 tttttcgctt ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc
    23821 attaccatta cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga
    23881 ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct
    23941 accgccagca tcccgaggga gcgggcaaat ttctgggtca gcatcccggt ttcgttgctg
    24001 gcaccggaag cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg
    24061 ttcttttcaa taaagttagc gtcacggtca gctttcatca gcttcgcaat acgggagaat
    24121 gcttcttccc agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag
    24181 cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg cccctttcgg gcacagcgcc
    24241 ccacggctta ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt
    24301 ttcgcgccat cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag
    24361 gtgttacgga tctctttagc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc
    24421 ttcggggcaa agcccaatgc cgctactgtt gttccagcca taccgcccgc gcagatttta
    24481 aaaaattgtc tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg
    24541 aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg
    24601 ccacaaaata atccgcctgc gcaaattgac ctacctcaat agcggtagaa aaacgcacca
    24661 ctgcctgaca ggccagttaa aaaaatgcta taaaattcag cttaattttt aacggcaaga
    24721 gagacaaaac agcgagcatg acacgacaaa aagcaacgct catagggcta atagcgatcg
    24781 tcctgtggag cacgatggta ggattgattc gcggtgtcag tgaggggctc ggcccggtcg
    24841 gtggcgcagc tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc
    24901 cgcgtattcg gcaaatcccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct
    24961 atgaaatctg tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag
    25021 tgggtatggt gaactatctg tggcccagcc tgacaattct ctttgccatt ctgtttaatg
    25081 gtcagaaaac caactggctg attgtaccag gattattatt agccctcgtc ggcgtctgct
    25141 gggtgttagg cggtgacaat gggttacatt atgatgaaat catcaataat atcaccacca
    25201 gcccattgag ttatttcctg gcgttcattg gtgcgtttat ctgggcagcc tactgcacag
    25261 taacgaataa atacgcacgc ggatttaacg gaattaccgt ttttgtcctg ctaacgggag
    25321 caagtctgtg ggtttactat tttctcacgc cacaaccaga aatggtattt agcacgccag
    25381 tcatgattaa actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaatg
    25441 tcggtatact gcatggcaac gtcaccataa tggcggtagg ttcgtatttt acgcccgtac
    25501 tttcctcagc gcttgcagcc gtgctgctca gcgccccgct gtcgttctcg ttctggcaag
    25561 gcgcgctgat ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggctaaa
    25621 ataattgccg ggtctacggc ccggcaatta atcagataaa ccccgacaat attaaaaaat
    25681 aaatttcaaa taccagtaat tcaccttata tctca
//
LOCUS       DAZGXS010000065        25265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGXS010000065 DAZGXS010000000
VERSION     DAZGXS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            56..796
                     /gene="yiaT"
                     /locus_tag="ACHKG5_002712"
     CDS             56..796
                     /gene="yiaT"
                     /locus_tag="ACHKG5_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP0868792.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(926..2896)
                     /locus_tag="ACHKG5_002713"
     CDS             complement(926..2896)
                     /locus_tag="ACHKG5_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP0868793.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2907..4307)
                     /locus_tag="ACHKG5_002714"
     CDS             complement(2907..4307)
                     /locus_tag="ACHKG5_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0868794.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4533..5348
                     /locus_tag="ACHKG5_002715"
     CDS             4533..5348
                     /locus_tag="ACHKG5_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0868795.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5350..5490
                     /locus_tag="ACHKG5_002716"
                     /pseudo
     CDS             5350..5490
                     /locus_tag="ACHKG5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5592..6065
                     /gene="ysaA"
                     /locus_tag="ACHKG5_002717"
     CDS             5592..6065
                     /gene="ysaA"
                     /locus_tag="ACHKG5_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0868796.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6217..7470)
                     /gene="avtA"
                     /locus_tag="ACHKG5_002718"
     CDS             complement(6217..7470)
                     /gene="avtA"
                     /locus_tag="ACHKG5_002718"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0868797.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7648..9678)
                     /gene="malS"
                     /locus_tag="ACHKG5_002719"
     CDS             complement(7648..9678)
                     /gene="malS"
                     /locus_tag="ACHKG5_002719"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0868798.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            9998..10822
                     /gene="bax"
                     /locus_tag="ACHKG5_002720"
     CDS             9998..10822
                     /gene="bax"
                     /locus_tag="ACHKG5_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0868799.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10930..12108)
                     /gene="xylR"
                     /locus_tag="ACHKG5_002721"
     CDS             complement(10930..12108)
                     /gene="xylR"
                     /locus_tag="ACHKG5_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0868800.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12186..13367)
                     /gene="xylH"
                     /locus_tag="ACHKG5_002722"
     CDS             complement(12186..13367)
                     /gene="xylH"
                     /locus_tag="ACHKG5_002722"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0868801.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13345..14886)
                     /gene="xylG"
                     /locus_tag="ACHKG5_002723"
     CDS             complement(13345..14886)
                     /gene="xylG"
                     /locus_tag="ACHKG5_002723"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0868802.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14964..15956)
                     /gene="xylF"
                     /locus_tag="ACHKG5_002724"
     CDS             complement(14964..15956)
                     /gene="xylF"
                     /locus_tag="ACHKG5_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0868803.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16322..17644
                     /gene="xylA"
                     /locus_tag="ACHKG5_002725"
     CDS             16322..17644
                     /gene="xylA"
                     /locus_tag="ACHKG5_002725"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0868804.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17716..19170
                     /gene="xylB"
                     /locus_tag="ACHKG5_002726"
     CDS             17716..19170
                     /gene="xylB"
                     /locus_tag="ACHKG5_002726"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0868805.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19339..19680
                     /gene="yiaB"
                     /locus_tag="ACHKG5_002727"
     CDS             19339..19680
                     /gene="yiaB"
                     /locus_tag="ACHKG5_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0868806.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19726..20163
                     /gene="yiaA"
                     /locus_tag="ACHKG5_002728"
     CDS             19726..20163
                     /gene="yiaA"
                     /locus_tag="ACHKG5_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0868807.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20205..21200)
                     /gene="wecH"
                     /locus_tag="ACHKG5_002729"
     CDS             complement(20205..21200)
                     /gene="wecH"
                     /locus_tag="ACHKG5_002729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0868808.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALILHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21375..21674
                     /gene="ysaB"
                     /locus_tag="ACHKG5_002730"
     CDS             21375..21674
                     /gene="ysaB"
                     /locus_tag="ACHKG5_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP0868809.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21769..22680
                     /gene="glyQ"
                     /locus_tag="ACHKG5_002731"
     CDS             21769..22680
                     /gene="glyQ"
                     /locus_tag="ACHKG5_002731"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0868810.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22690..24759
                     /gene="glyS"
                     /locus_tag="ACHKG5_002732"
     CDS             22690..24759
                     /gene="glyS"
                     /locus_tag="ACHKG5_002732"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0868811.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            25082..25234
                     /gene="hokA"
                     /locus_tag="ACHKG5_002733"
     CDS             25082..25234
                     /gene="hokA"
                     /locus_tag="ACHKG5_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP0868812.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT         6264 a         6192 c         6510 g         6299 t
ORIGIN      
        1 gtaagcatta ctatgccgac tcttttttat cttattttaa aatggaatac cggacatgtt
       61 aattaaccgc aatattgtgg cgttatttgc gttgcctttt atggcaagcg caactgcttc
      121 tgaattatcc attggtgctg gtgcggctta taatgaatcg ccttatcgcg gttataatga
      181 aaatacaaag gcaattccgc tgattagtta tgaaggtgat tctttttatg ttcgtcagac
      241 cacgttaggt tttattctgt cgcaaagtga aaaaaatgaa cttagcctga ccgcatcctg
      301 gatgccgctg gaatttgacc ctgccgataa tgacgattat gccatgcaac agcttgataa
      361 gcgtgatagt acggctatgg cgggggttgc ctggtatcac cacgagcgtt ggggaaccgt
      421 gaaagcctct gcagctgcgg acgttctgga taacagcaac ggctgggtgg gggagctatc
      481 ggtattccac aaaatgcaga taggtcgtct gtcgctgaca cctgcgctgg gcgttctcta
      541 ttatgacgag aatttcagtg actattacta tggcatttca gagagtgagt cccgtcgtag
      601 cggtctggca agttattccg cgcaggatgc ctgggtgccc tatgtcagcc tgacggcaaa
      661 atacccgata ggagagcacg tcgtattgat ggcgagcgca ggatacagcg agctgccgga
      721 agagattacc gacagcccga tgattgatcg taatgagagt tttacctttg tcaccggcgt
      781 gagctggcgt ttttaattca ccggtggatg tcggtgcggc tggcctacgc tctcgcaccg
      841 acttgtatgc ctgataagac gtgttaagcg tcgcatcagg caatgtgctc cttacgttgg
      901 atgcggcgcg agtgccttat ccaacctacg gatggcaatg cttttcctca ttcacccaga
      961 tccgcatttc gccttcaccc cggttggccc agctaaacca cgggataaag gtcagagttt
     1021 gcgtctggcg tttggctggc gcgctgtcgt aatgccacag cggttgctgc tctggattgc
     1081 tctgttcata ccggtaaccc ggtgcctgga ttaagatctt atggctaaac aatcccttgc
     1141 cttcaaatgt cgtaaatggc gcatcggtgg gcagccacag attatgcagt gactcgccgt
     1201 tgtcggcctt ttccaggcaa tacaccagcg ggccgcgctg aatcgccact tttccggcga
     1261 cgtgacgcac cagcgggtta ccgtaaacgc ggcgtaccgg catcggcaaa gtcagattca
     1321 gcgtatcgcc ctcctgccat tcgcgggtaa tgtgcaaata ccctttacga atatcctgct
     1381 cgacctcttc cccattcaat atgatctgcg gctgtgtgca ccagtccggc agacgtaaag
     1441 ccagcgtatg acgtaccggc tggggcgatt caaccgcaat cgtcacctgc tcctgccacg
     1501 gatagttccc gctaacccgc aggcgcagcg tgccattttc taccggcact tccatgctgt
     1561 ttcctgcgta gatgttgata tacaacgcat cttcacgcgg cgtgtagaga taatgaccaa
     1621 tcgaggtcag cacgcgggcg atatttggcg gacaacaagc gcagccaaac caacgctggc
     1681 ggatcggttt aacgtgatcg tagatatggt tgaatttcag cgattttgga tgtacttcca
     1741 gtggattcac atagaagaaa tgtttgccat ccaatgccat gccgccgagc acagtgttgt
     1801 acagtgcgcg ctccatcaca tcggcatatt gactgtcgcc ttccatttcc agcattcgtc
     1861 gggcgaacat catcaggccg atggaagcac aactttcggc gtaaaccgtg tcattcggca
     1921 gatcgtaatc gctgctgaac gcttcgccgc tgctttgtga gccgatgccg ccggtaatat
     1981 ataactgacg ctgggccata ttgttccaca gccgcaggca gtcctgacgc ttgctgtcat
     2041 cgtgacttaa acgcgcgaga tgcgcgacgc cggtcatcag atagacaaaa cgtaccgcgt
     2101 gaccgatggc ggtttgctgt tgcgcaaggg gcaaatgtgc ctggctgtag gctttgtctt
     2161 tcaccatcca cgccgggccg taggtgtgcc agtgcgatgt ctgcccgcgc ttttcatatt
     2221 cctggtcgta atagtgcggt tgcgcaccac gctgttcgac aaaatagttc gtcagcgcca
     2281 ggtagcgcgg ctcttcagtc acttcataca ggcgcattag tgccagttca atttccgggt
     2341 gaccaggata accgtgtaac ttactttcat ctggaccaaa tacgctgtcg atatgatcgg
     2401 ccaggcggca aacgaccccc agcaagcgcc gttttcccgt ggcctggaag aaggcgactc
     2461 cggcttcaat cagatgacct gcgcagtaaa gttcatgaca ctccgccaga ttgctccagc
     2521 gttcttcggg tgcttttacc gtaaagtaag tattgagata gccgtcttcg cattgggcgg
     2581 aggcgatcag ttcgattacc tcgtcggcgg ttttttccag ttcggcgtcc ggcttctggc
     2641 acagcgacca ggctaccgct tccagccatt tggcgacgtc gctgtcctga aacaccatcc
     2701 cgtaaaattc accctcctga agtccggcgg caatgcgaaa gttttcaatc gcatggctgg
     2761 gttccgcttc tgggatacga tcgttcaagg catcccactg ataaggaatc accacgtcgc
     2821 ggaccagttg ttggtactga ccgaggaacg gatcgctgac cgtcagttta tgcagatcga
     2881 cttccgaaat gttcatcttg tgctccttaa ggctgtacat gacgctgacg caggtcggta
     2941 gctatgcgtg acatcatagg attattgagt ttgcaaaagc ggatcgcccc tgccagcagc
     3001 aggtggaata gcgcaggcag taatgtttcc atcgccgcga tcccgccaag cgaactggcg
     3061 gtctggtttt ccacgccagg ctggtaggcg acaaaaataa acagcaggct gatgatcccg
     3121 gcgctggagg cccaggccag cttgatgaaa aacagattga aggcgaagtt catgccggaa
     3181 gaacgtacgt gggttttcca ctcgccgtag tcatcggcaa aggccatcaa tgagaagtgc
     3241 agtggcaatg tgaagccaag aatcacgcca ttaccgagga tcaccgccag ccacagcgtt
     3301 tgataagccg ggccggaggg caggaaccac atcaacaccg ccagtgcagc gaggagcagg
     3361 ttggtgtagt aataaatttt gacggtatcg aaacgccgcg ttaacgggct gacaatcacc
     3421 gagccgataa tggaggcgaa ggtgaccatg gtgaagaaca gcgacgtgta gcccgtgctg
     3481 ccttgtaaga cgtaggtaat gaagtacata tacccaccgc cgcgaatgtt aaagacgtta
     3541 atcagcagga aagacatgac cagcatcagc agcagttggt cgttgttccg cagcccggca
     3601 agatgttcgc gcagggtaaa tttccccatt gtggagagcg gcacccgttc acgaacccag
     3661 aagaaacagc acaggaacat caccacggca atggcgcaca ataccccgac gcccagttga
     3721 tagccgcgtg cagcgttgcc ctgaccgaag agagctacca tccacggtaa gcctaccgaa
     3781 accagaaatc ccgctacgcc gcagagaacg aatcgccagg actggcagga gatcacttca
     3841 ttgtggcggg tggtcatggt gttgatccgc gcgcagtacg gtacgttgat ggcggtataa
     3901 ccgactgaaa ggagcaggta agtaccgaac gcccaggcga tttttactcc catgctggca
     3961 tctggcacgg tgaacgtcag gatgccgata atgccgatgg gaatggcaat ccatagctgc
     4021 cacggacgaa agcgccccca tcggctttgc gtccggtcgg caatgacgcc catcaccggg
     4081 tcggagatgg catcaaacac gcgcagtgca ataaacagcg tgccaaccag cgccggagtt
     4141 aatccaaaaa cgtcggtgta gaaaaaggtg agaaaattca tgatcaggca agttattacg
     4201 gtgccgcctg cgtcacccag gccgtagcct attttttccc gtacagataa cgcatcgtca
     4261 attttacctt tagcgatatc agcggtagta atcggtgtag aggtcataga tattcctcgt
     4321 gaatagggag caatttttag ttttattgaa atgacctgat taattctgcc gtgaacggga
     4381 atatcaacaa ggcgagaaaa gagtaaaaaa aggtgacgat tccgacctga atttaaaagt
     4441 gtgaaatgga tcaggtgaaa ttaatgtttt tgattaataa tcaaaaggta atgtgtttaa
     4501 tcattaatga ataagcggga aattacatat caatgctcga attatccatg acgttaccga
     4561 ttaaagtgca aaatggtgga ttatttattt cccgtggcgt agggaggcat tctgcaaggc
     4621 ggctggagtc atgggaaatt atctttgtcg aaaaaggtcg attaacgatt caggaagaag
     4681 aacggatatt tctggtcgat gcgggagaga gtttattact ctggccgaac cgacaacatg
     4741 ttggggtgga ggattttccc gccgatctca aattttactg gctgcatttt gaggttaaag
     4801 cgccggacaa cgaccctcgc tggcttactc atcttagcgt gccgcaacaa acgaaggttg
     4861 tcgatccgca ggcgctggtg gccctgtttc gccagttctt aagcgaacag gaaaagcatc
     4921 aacgcactcc ggcgctggag tttattgtgc tgctcatttt gcagcagctc acccgtgacg
     4981 cgcgtcttga tgaacaggcc gatggcgcag gcaccaccct tgcctggaaa gcgcagaaac
     5041 tgatccgcac gcaatatcat cgcccgcttt ctacggcgct tctggcccgc gagctgtact
     5101 gcaatgccga ttatcttggg cgcgtctacc ggcgggtgtt tcatctgacg ttaacagaag
     5161 caatccatcg ccagcgggtg cgcgaagccg aaaagctgtt aattagcgaa gcgcgatcgc
     5221 tcacggaagt ggcggcgttg tgcgggttta atgacgttgg ctatttccgg cagatttttc
     5281 gcaaacacac cggtttaacg ccagcagcgt ggaagcggcg ttacagcaag gaacatatca
     5341 attcgtagtg ccggggcgat ttcgctttcg acatcacgtc tgaaacaggt gggggagaaa
     5401 aatctcctga aaccactgcg tgaaaccgcg caggctattt ctaatgaact gggatttact
     5461 gtcagggacg accagggcgc gatcacataa cgctttggac aagtgccaaa actttaacat
     5521 ttccttcgtt ggatcaaagc agtagggacg cgctctctgg cactctgctg ttttagtgca
     5581 aaggagtgat catgaaccgg tttattattg cggatgcgac gaaatgtatc ggttgccgta
     5641 cctgtgaagt ggcttgcgca gtgtcgcatc aggagaatca ggattgcgct gcgttgtcac
     5701 cagacgagtt tatttcccgt attcgtgtca ttaaagacca cagctggacc acggcagtag
     5761 cctgtcatca gtgtgaagat gcaccgtgcg cgaatgtctg ccctgttgac gcgataagcc
     5821 gcgaacatgg gcatattttc gttgaacaaa cacgttgcat tggctgtaaa agctgtatgc
     5881 tggcttgccc gtttggtgcg atggaggtcg tttcttcgcg caaaaaggcg agggcgatta
     5941 agtgcgatct gtgctggcat cgggagacgg gaccggcctg tgttgaagcc tgcccgacaa
     6001 aggcgttgca gtgcatggat gtcgagaaag tgcagcggca tcggctacgg cagcagcctg
     6061 tttgaaacgt ttttccggcg tttctcaacg ccggaatagt gcgctttgtt tatgccagat
     6121 gcggcgtaaa cgccttatcc ggcccacaaa ttcttgcaaa ttaaatatat tgcagaaatc
     6181 atgtaggcct gataagcgta gcgcatcagg caatttttag tgactttcag cccaggctct
     6241 ttctatctct tccgccagaa tcttcacccc cgcctcaatt ttctccggct ctggtacgta
     6301 gttcatgcgc atacattgat gcgtatgcgg ccacggtttg tccagccctg ggaagaagtt
     6361 gtgccccggc accatcagca cgccgcgtgc tttcaggcgc tgatagagct gctcggtcgt
     6421 aatgggcaaa tccttaaacc aaagccagag gaaaatggct ccttctggtt tatgaattag
     6481 gcagcgatct tccggtaaat agcggcgaat gatggcgata gtttcctgaa cacgctggta
     6541 gtaaaacggt ttgatgactg tttcagacag gcgcagcaga tcgttacgct taatcatttc
     6601 acacatcatc gccggaccaa taccgccagg tgccaggctg ataatgccgt tcatattggt
     6661 gatggcggtg atgatttttt cattggcgat gataatgccg cagcgggagc caggcagccc
     6721 cagcttggaa agactcatgc acagcacgat attcggattc catagcgggc gcgcttcact
     6781 gaagatgata cccgggaacg ggacgccata agcgttatca atcaccagcg gaatgccgtg
     6841 ttgattcgcc agcgcgtcaa gcttcagcaa ctcttcgtca gtaatcacat tgcctgttgg
     6901 attcgtcggc cgggagacgc aaatcatccc ggtttcttcg ccaatatgca gatgctcaaa
     6961 atcgacgtgg tatttaaact ggccttccgg cagcagttca atattcggac gcgcagagac
     7021 aaacagatct tcttccagtc cggcgtcagc gtagccaatg tattccggtg ccagcgggaa
     7081 cagcactttt ttgacccgac catcggcacg gcgtccggca aacagattaa ataagtagaa
     7141 aaacgcgctc tggctgccgt ttgttagtgc aatattctgt gcttcgatat cccaacccaa
     7201 cttctcgcgc agcattccgg caagcagtgt gagtagctcc gttttcccct gtggaccgtc
     7261 gtaattgcac agtgcatcag tcgctttgcc actttccagc atgtcggtca gtagcgtctg
     7321 gaagtagtcc tgcatttccg ggatctgcgc cggattaccg ccgccgagca taatcgcgcc
     7381 aggcgtgcgt aaaccgtcgt tcagatcttc catcagcagc gtaatgccgg agtggcgggt
     7441 aaatttgtca ccaaaaaggg agaatgtcat agcgggatat ctgtcgaact ctgtagcaag
     7501 gaaggtaaca ataacgctac atttgctggg gtgcaaatcg gtctgttgaa gagtgaacgg
     7561 tgctttatcc tgcaacgctg attagggctg acattttatc ctggttggcg gcttcccgag
     7621 gtactgacga acgggaagcc ggaaaagtca ctgttgccca gcccagatga ccagcacttt
     7681 atcgtcgcca tgctcacgaa caaagccgta gccctgcttc agcgaaagtg tcgtttgttt
     7741 gcccgcgcca attgcgggat ggcgggcgcg gaactggctg attttctgcc agtgcgcgac
     7801 gttggcggca gatttaccgc taacatcctg ccagttcata tccgaacgtg taccttgcag
     7861 cggatcagaa cctgtaggac cgaacggacg cgaggattca tcgccataaa agatttgtac
     7921 cgcgcctggc gctaatagta ataactctgc tgctttgtcg cccccttcac ggaacagacg
     7981 ggtatcatgc gacgagaggt agctcaacac gttgaaaccc tgcaatttct ccgccatttg
     8041 ctgccaggtc gtatccatct gcgcaataca atcgacagct ttcgccgcct gctcctgata
     8101 atcgaaattg atcatcgcat cgaagccgtg gcgatagtag tcactttgca tcacgccgtg
     8161 gccccaggct tcaccggtca tccagaaagg tttgtcatct aatgctttgt cggggttagc
     8221 ttttttccat tcgcgaagcg cggcgctggc ttcggttttc agttgctgcc aagcgggcaa
     8281 ctcaacatgt ttggcggtat cgacccgaaa accatcaatc ccatagtcgc ggacccactg
     8341 acttaaccag tgggttaagt aatcgcgagg ggtaaagccg tcgatggctt tagcgtgggt
     8401 atccgttttg tttttataga acaccggcag accagaagcg gtagttgatt cggttttgat
     8461 atccggcaaa aaggctagcg acatggtgag atcgtcgaat ccaggattgt cgtaatcgcc
     8521 gatatcggta cggatccagt tttttcccca ccatttatcc cagcctgttt tgtcgctgaa
     8581 attaatgtaa tcgttaaagc tatgccaggt ttgcccggcg gcaggtttcc agtcgctcca
     8641 gcgttcaccc agcgtttttt tcacttcgtc accagaaaga tataacgcgc caaactgata
     8701 ctcctgcata tccgccagcg tggcatagcc ggtgtggttc atcacgacat caaagagaat
     8761 acgaataccg cgctgatgtg cgctatcaac cagcgtccgt agatcggctt cgttgcccat
     8821 attggcatca agattcgtcc agtcctgtgt gtaataaccg tggtaggcat aatgcgggaa
     8881 atcgcctttt gtaccgccgc cgacccagcc gtgaatttgc tcaaatgggg cgcttatcca
     8941 taaagcatta acgcccaact gctggaggta atccagtttg ttggtcaggc cgcgtaaatc
     9001 gccgccgtga aaagtgccaa tttccgccat accgtcttta tgacgtccgt aactctggtc
     9061 attactggga tcgccgtttt cgaaacgatc tgtcagcaca aagtaaaccg tggcgttatg
     9121 ccagtcgaaa ggggcagagg cgtcagtttc tgcccgttcc agcaggagta aaccgttgct
     9181 ggtagcagcg ggttgtaacg ttatttgacc gttcttcact atcgcaattt gctggctgta
     9241 ataatcccgt acggcggctc cttccgggaa agtggcgctg acatccagcg tgagcggtaa
     9301 tccatcccat ttcgggcatt cacggaccag gtttgctacc ggttcggcgg cgttctggat
     9361 ggaaatcatc aatgttggcg taccggagcg ggtgtctatt tgcagcgtat atttgccgtc
     9421 cctgaacaat cgccattgag gcggcgtgtt gctacaaggt tgcagggaaa gcatctgatt
     9481 gagttttatc gcatctgcag gctgccagca ctgctggtca aaatttagcg tgagtggacg
     9541 cgtacctttg ggcaactgcg cgtggctgac aaatgttccc gttccctgtt cgctaaaggc
     9601 agggaacccc ggagaagtcc agctggcggc aacggcgaag ccaggaagga gtgtcagaaa
     9661 acaggcggcg agtttcatgg tggatgagtc cttattgctg cgatttcaga cagtttgcca
     9721 gcgataagcc agacaaaact catccctgag cggtaagtcc acaggatgag aagcaagggt
     9781 gagcgatcgc gcgcaaaaaa cggctgaatt ttgcgataaa ccccacattt tctgcgattt
     9841 agcgccaatc tgaatcgtta actcgtgaca tagtttcaga tttggactat tccttggggt
     9901 gctcttgaca gctatttttg atatgatttg agattcaact ctcaaatttg tgaaaaatat
     9961 aaggtgttgg aatgattaaa tccgaccagg agacctgatg attttgactc ccatacgacg
    10021 atatggggcg atgattctta tgttactcac tctggtgttt tcgagtgagg tgttagcgaa
    10081 gacgcacaca acaacagcga gtcaaaagtc ccacttaact aaagctagta ataaacaggt
    10141 aagcagtaaa caagagtatt ctcgcaatag tgcaaagagt agttcacttc ctgatttgcg
    10201 aaaataccct tccggaacat caaggaaaaa ggcgtttctc cggaccgtaa tgccttacat
    10261 taccagccaa aatgcagcca ttactgcaga acgtaactgg ctgatttcaa aacagtatca
    10321 gggccaatgg tcacctgctg agcgtgcgcg tctgaaagac atcgccaaac gctacaaggt
    10381 gaagtggtct ggtaatacgc gaaaaatccc gtggaatacc ttgcttgaac gcgtagacat
    10441 tatccctacc agtatggtgg cgacgatggc tgcagcagaa agcggttggg gaacgtcgaa
    10501 gctggcgcgc aacaacaaca atcttttcgg catgaaatgc atgaaaggac gttgtaccaa
    10561 tgcgccaggt aaagtgaaag ggtactcaca gtttagttct gtcaaagaat cggtgagcgc
    10621 ctatgtcact aacctcaata cgcatccggc ttactcttcg ttccgtaaat cgcgtgcgca
    10681 gctgcgtaaa gctgatcagg aagtgactgc cacagcgatg attcacaagc tgaagggcta
    10741 ctcgaccaaa gggaagagtt ataacaacta cctgttcgca atgtaccagg ataaccaacg
    10801 gttaatcgcg gcgcatatgt aattgtattt cctcgagcct tatccgactt gtcggtcgga
    10861 taaggctttt gatcgcatca ggtggttgtg ctgagttacc tgatgcgacg ccgacaattc
    10921 tcatcatcgc tacaacatga cctcgctatt tacatcgcga tactcttttg gcgtcgtgtc
    10981 atatgctttt ttaaaaacag agtagaaata ttgcagcgat ggataaccgc acatttgcga
    11041 tatctcattg atcgacaagg tggttgaaat cagcagactg cgcgctttct ccagcttctc
    11101 agcatgaatc atggcatgga tggtttcacc cacctcttct ttaaaacgct tctcaagatt
    11161 ggagcgcgag atcccgaccg catcgagtac ctgatccact ttaatccctt tacaggcgtg
    11221 attacgaatg taatgcatgg cctgaataac ggcgggatcg gtcagcgaac ggtaatctgt
    11281 tgagcgccgt tcaatgacgc gaactggagg gaccaaaatc cgctgtagcg gcatttcttc
    11341 tttatctaat aatcgatgca acagttttgc cgcctgatag cccatttgcc gcgcgccctg
    11401 agcgaccgaa gaaagggcga cacgcgacag atagcgggtc agttcttcgt tatcgatgcc
    11461 aatcacgcat aatttttccg gtacgggaat gtgtagatgt tcacatactt gcagaatatg
    11521 ccgcgcccgg gcgtcagtaa cggcaataat cccggtttgc ggtggcagtg tttgtagcca
    11581 gtctgccagc cgattttgcg cgtgttgcca gttctctggt gcggtttcta acccctgata
    11641 aaccactccg cgatactttt cttcggcgac aagctgacga aatgcatatt cgcgttcagt
    11701 ggcccaacgt ttgccgcttg attccggaag accataaaaa gcaaagcggt taacgccttt
    11761 ctcttttaaa tgcaaaaatg cgctttcaac cagcgcatag ttatcggtgg caatgtaatg
    11821 aacgggtggg taactttctg caaggtgata cgagccgcca accccaacaa tggggacgtc
    11881 gacatcagcc agcgcttgct cgatctgttt gtcgtcgaag tcggcaatga cgccatctcc
    11941 taaccagtcc ttgattttat caatgcgggc gcggaaatct tcttcaatga aaatatccca
    12001 ttccgattgt gacgcctgta aatattcccc tacgccttct actacctgcc ggtcataggc
    12061 tttattggca ttgaacagta atgtgatgcg gtgacgttta gtaaacatgg ttcttttcct
    12121 gctgaatcat gcaaaaactc aaaaccggta atacgttacc ggctttgaga aaatttttat
    12181 caaaatcaag aacggcgttt ggttgcggag tccatccata ctgccagcaa cagaatcgca
    12241 cctttaacga tatactgcca gaaggtcggt acatccatca tactcatacc gttatccagt
    12301 gaagccatga tgaatgcccc cattaccgct ccagccacgc tcccgacgcc gcctgccaga
    12361 ctggtgccgc caatcacgca tgctgcaatt gcgtccagct cggcgatatt ccccgcagaa
    12421 ggtgaaccag cgccaagtcg agaactgagg attaatccgg cgatggctac cattaatccg
    12481 ttaatagcga acacggcaag tttggtgcgt tcaacgttaa ttccggagag acgtgctgct
    12541 tccagattgc cgccgatggc ataaatgcgt cgtccaaatg ctgtccgcgt tgccataaac
    12601 attccgccga gtaacagcaa cgtcagcagc agaacaggag taggaacgcc acggtaatca
    12661 ttcaacagcc agattgcgcc taatacgatg atagcggtta aagcctggcg accgactact
    12721 gcggtagagg ctggagactg caaacccaaa gcctgacggc gcattcttcc gcgccattgc
    12781 cagccaacaa aagccattaa gccaagcgcg ccaatgatga agccggtgct ggcggggaga
    12841 tagctttgcc caatttgtga catcgcggcg ctggtggggg atacagtcgt gccgttggtg
    12901 atgccaatga gtatgccgcg aaatgccaac atgcccgcga gagtgacaat aaatgaaggg
    12961 actttgcggt acgcgaccca ccatccgttc caggcaccga gaagcagtcc cagaaccaac
    13021 gtcacaatga tggtaagtgg caaaggccag cctagccaga cgtcacaaat cgccgcgacg
    13081 ccacctaata gccccatcat tgagccgacg gaaaggtcga tttcagcaga aattatgacg
    13141 aacaccattc ctaccgcgag gatgccggta atcgcggtct ggcgtaacag gttggagacg
    13201 ttacgggcgc ttaagtaggc accatcggtg gtccaggtaa agaacagcat gattgcgatg
    13261 atagctgcaa tcatcacgaa gacctgcaaa ttcagtgatt tcagcccgga gaagccaccg
    13321 gatgtcggta cggccaattt cacttcagac ggattgcttt tcgacatgat gttcgctcct
    13381 caatgcggct tccatcacct gctcctgagt caggttatga tttatcaggt tggcttttag
    13441 tttcccttca tgcattacta gtacacgatc gctaaggccg agcacttcag gtaattcaga
    13501 agagatgaca ataacggcaa taccctgctg gacgagttgg ttaattaatt tgtagatctc
    13561 gtatttcgcg ccaatatcga tacccctggt gggttcatca agaatgagaa tgcgcgggtt
    13621 aagtaacaga cagcgagcga ggatcgcttt ttgctgattg ccgccgctca aacgtccaat
    13681 agcaaggtcg ggggacgacg ttttaacttt gagttgctgg attgattcca gaatacattt
    13741 ttgctctgcc gcgtcatcaa gctggctaat gccaccggta aatttattga gtgcggcgag
    13801 ggtaatattt ttaccaaccg ccattaccgg aacgatgccg tcgcgctttc tgtcttcggg
    13861 gaccatcgca atcccctggg cgatggcttg ctgacagtta cgaatatcta cctgtttgcc
    13921 atcaatataa atttttcctt cccattgtcc gggccacaca ccaaacaggc actgaatggt
    13981 ctcggtacgt ccggcaccaa cgagtccggc aatacccagt atttcgccac gtttcaggga
    14041 aaacgagaca tcattaactc gtttaatatg acgattaacc ggatgccatg ccgtcagatg
    14101 ttcaatacgt aatatttcat ctccggtggt atgtggttca ttagggtaaa gcgcggttaa
    14161 ctctcgcccg accatcatgg tgataatatc gtcttcactc attccggcag catcacgcgt
    14221 accaatgtgc tgtccgtcgc gaataacgca aatcgtatcg gaaatcgctt tgacttcgtt
    14281 gagtttgtgc gaaatataaa tacaggcgat accgtgctgt tgtagatcgc gaataatatc
    14341 cagtaaaacc gacgtttcct gctcagttaa tgaggctgtc ggttcatcga gaattaacaa
    14401 gcgcacctgt ttattaagtg ccttggcaat ttcaaccagt tgttgttgcc caagccctaa
    14461 atcgccaacg cgggtatcag gtgaaatgga taaactgacc tgtgcgagca gcttctgaca
    14521 gcgtagcgtc atcaggtcat aatccataat gccattgtgg gttatttcgt tacccaggaa
    14581 gatattttcc agcacggtca attctttcac cagggccaat tcctggtgaa tgatggcgat
    14641 acctttgcgt tcggtatcgc ggatgtgact cgcctgaatc tcttctcccg caaaaataat
    14701 ttcgccttcg taggagccat ggggataaat accacacagc actttcatca gcgttgattt
    14761 accagaccca ttttccccac agagtgagac gatttcgcca gcattcaacc gcaagctgac
    14821 gttatcaatc gccttcacac tgccgaaggt tttggtaatg ttcttcattt caagtagata
    14881 aggcatagcg actccaccta agccaattca ttcacgcggc atggagagaa atcacgcccc
    14941 cgctccgcgc tggggcgtaa cgcttacagc tcgctctctt tgtggaatcc gtctttaatt
    15001 accgtatctt tgatgttgtt tttattcaca tcgatcggtg tcaggaggcg ggaggggaca
    15061 tctttcaggc cattatttag tgaagtatct gcttttggct cctgaccatt gcccaattca
    15121 acggcaattt ctgcggcagt atttgccagc aatgtaatag gtttatatac cgtcatagtt
    15181 tgcgtaccgg cagcaatacg tttaatacct gcgagatccg catcctggcc agaaattgct
    15241 actttccctg ataaaccttg cgcgcttaat gcctgaattg ccccacctgc ggtggcatcg
    15301 tttgaggcaa ctacagcatc aattttgtta ttattggcgg ttagcgcgtt ttccataatt
    15361 ttcaatgcgt tttccggtaa ccagccatca acccattggt caccaacgac tttaattttt
    15421 ccggaatcaa cgtaaggttg taacactttc atttgtccgg cgcggaacag cttagcgttg
    15481 ttatctaccg gcgagccgcc catcaggaag taattacctt gcggaacaat atcgaccagg
    15541 gcttttgcct gcagttcacc gactttttcg ttatcgaaag aaatataaaa atcgatatcc
    15601 gcatcgttaa tcatacggtc gtaagctaat actttaatgc cttcttgttt ggcttctttt
    15661 acaacgttac ttaatacctg accgttatac ggaataatga caagaacatc gacaccccgg
    15721 tttatcatgt tttcaatctg cgacatttgt gtttcttcat tgccatttgc agactgtaca
    15781 aatactttcg cgccgagaga ttctgccttt ttcacaaaga tatctcgatc tttttgccag
    15841 cgttcaagac ggagatcatc aatcgccata cctattttga cttctttggc gtgtgcagca
    15901 acgttggtaa gcaggagtga ggtgcaaagg gtgagtagaa tgttctttat tttcatggtg
    15961 tagggccttc tgtagttaga ggacagcttt aataagtaac aatcaccgcg ataaacgtaa
    16021 ccaattttta gcaactaaac aggggaaaac aattacagat ttttatcttt cgattacgat
    16081 ttttggttta tttcttgatt tatgaccgag atcttacttt tgttgcgcaa ttgtacttat
    16141 tgcatttttc tcttcgagga attacccagt ttcatcattc cattttattt tgcgagcgag
    16201 cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc aactgaaagg
    16261 gagtgcccaa tattacgaca tcatccatca cccgcggcat tacctgatta tggagttcaa
    16321 tatgcaagcc tattttgacc agctcgatcg cgttcgttat gaaggctcaa aatcctcaaa
    16381 cccgttagca ttccgtcact acaatcccga cgaactggtg ttgggcaagc gtatggaaga
    16441 gcacttgcga tttgccgcct gctactggca caccttctgc tggaacgggg cggatatgtt
    16501 tggtgtgggg gcgtttaatc gtccgtggca gcagcctggt gaggcactgg cgttggcgaa
    16561 gcgtaaagca gatgtcgcat ttgagttttt ccacaagtta catgtgccat tttattgctt
    16621 ccacgatgtg gatgtttctc ctgagggcgc gtcgttaaaa gagtacatca ataattttgc
    16681 gcaaatggtt gatgttctgg caggcaagca agaagagagc ggcgtgaagc tgctgtgggg
    16741 aaccgctaac tgctttacaa accctcgcta tggcgcgggt gcggcgacga acccagatcc
    16801 agaagtcttc agctgggcgg caacgcaagt tgttacagcg atggaagcaa cccataaatt
    16861 gggtggtgaa aactatgtcc tgtggggcgg tcgtgaaggt tacgaaacgc tgttaaatac
    16921 cgatttgcgt caggagcgtg aacaactggg ccgctttatg caaatggtgg ttgagcataa
    16981 acataaaatc ggcttccagg gcacgttgct tatcgaaccg aaaccgcaag aaccgactaa
    17041 acatcaatat gattacgatg ctgcgacagt ctatggcttc ctgaaacagt ttggtctgga
    17101 aaaagagatt aaactgaaca ttgaagctaa ccacgcgacg ctggcaggtc actctttcca
    17161 tcatgaaata gccaccgcca ttgcgcttgg cctgttcggt tctgtcgacg ccaaccgtgg
    17221 cgatgcgcaa ctgggctggg acaccgacca gttcccgaac agtgtggaag agaatgcgct
    17281 ggtgatgtat gaaattctca aagcaggcgg tttcaccacc ggtggtctga acttcgatgc
    17341 caaagtacgt cgtcaaagta ctgataaata tgatctgttt tacggtcata tcggcgcgat
    17401 ggatacgatg gcactggcgc tgaaaattgc agcgtgcatg attgaagatg gcgagctgga
    17461 taaacgcatc gcgcagcgtt attccggctg gaatagcgaa ttgggccagc aaatcctgaa
    17521 aggccaaatg tcactggcag atttagccaa atatgctcag gaacataatt tgtctccggt
    17581 gcatcagagt ggtcgccagg agcaactgga aaatctggta aatcattatc tgttcgacaa
    17641 ataacggcta actgtgcagt ccgttggccc ggttatcggt agcgataccg ggcatttttt
    17701 taaggaacga tcgatatgta tatcgggata gatcttggca cctcgggcgt aaaagttatt
    17761 ttgctcaacg agcagggtga ggtggttgct tcgcaaacgg aaaagctgac cgtttcgcgc
    17821 ccgcatccac tctggtcgga acaagacccg gaacagtggt ggcaggcaac tgatcgcgca
    17881 atgaaagctc tgggcgatca gcattctctg caggacgtta aagcattggg tattgccggc
    17941 cagatgcacg gagcaacctt actggatgct caacaacggg tattgcgccc tgccattttg
    18001 tggaacgacg ggcgctgtgc gcaagagtgc gctttgctgg aagcgcgagt tccgctatcg
    18061 cgggtgatta ccggcaacct gatgatgccc ggatttactg cgcctaaatt gctatgggtt
    18121 cagcggcatg agccggagat attccgtcaa atcgacaaag tattattacc gaaagattac
    18181 ttgcgtctgc gtatgacggg ggagtttgcc agcgatatgt ctgacgcagc tggcaccatg
    18241 tggctggatg tcgcaaagcg tgactggagt gacgtcatgc tgcaggcttg cgacttatct
    18301 cgtgaccaga tgcccgcatt atacgaaggc agcgaaatta ctggtgcttt gttacctgaa
    18361 gttgcgaaag cgtggggtat ggcgacggtg ccagttgtcg caggcggtgg cgacaatgca
    18421 gctggtgcag ttggtgtggg aatggttgat gctaatcagg caatgttatc gctggggacg
    18481 tcgggggtct attttgctgt cagcgaaggg ttcttaagca agccagaaag cgccgtacac
    18541 agcttttgcc atgcgctacc gcagcgttgg catttaatgt ctgtaatgct gagtgcagcg
    18601 tcgtgtctgg attgggccgc gaaattaacc ggcctgagca atgtcccagc tttaatcgct
    18661 gcagctcaac aggctgatga aagtgccgag ccagtttggt ttctgcctta tctttccggc
    18721 gagcgtacac cacacaataa tccccaggcg aaaggggttt tctttggttt gactcatcaa
    18781 catggcccca atgaactggc gcgagcagtg ctggaaggcg tgggttatgc gctggcagat
    18841 ggcatggatg tcgtgcatgc ctgcggcatt aaaccgcaaa gtgttacgtt gattggtggc
    18901 ggggcgcgta gtgagtactg gcgtcagatg ctggcggata tcagcggtca gcagctcgat
    18961 taccgtacgg ggggggatgt ggggccagca ctgggcgcag caaggctggc gcagattgcg
    19021 gcgaatccag agaaatcgct cattgagttg ttgccgcaac taccgttaga acagtcgcat
    19081 ctaccagatg cgcagcgtta tgccgcttat cagccacgac gtgaaacatt ccgtcgcctc
    19141 tatcagcaac ttctgccatt aatggcgtaa acgttatccc ctgcctgacc gggtggggga
    19201 taattcacat ctatatatct cagcaattaa ttaatattta gtatgaattt attctgaaaa
    19261 tcatttgtta atggcatttt tcagttttgt ctttcgttgg ttactcgtaa tgtatcgctg
    19321 gtagatatgg agatcgttat gaaaacctca aaaactgtgg caaaactatt atttgttgtc
    19381 ggggcgctgg tttatctggt tgggctatgg atctcatgcc cattgttgag tggaaaaggc
    19441 tattttcttg gcgtgttaat gacagcaact tttggcaact atgcatatct tcgcgcagaa
    19501 aaactcgggc aactggataa tttttttacc catatttgcc agttagttgc gttaatcact
    19561 atcggtctct tgtttatcgg tgttttaaat gcacctatca atgcttatga aatggtgatc
    19621 taccccatcg ccttttttgt ctgcttgttt ggtcaaatgc gtttgtttcg ctcggtatga
    19681 gcaacataaa gctcttacac attcaggaat gaaaggaata ctgtgatgga caacaaaata
    19741 tcaacctatt caccggcctt tagtattgtg tcatggatag ctctcgttgg tggtatcgtt
    19801 acctatctgt tagggctatg gaatgcagag atgcagttaa atgaaaaagg atattatttt
    19861 gccgtactgg tattaggact gttttctgcg gcgtcttatc aaaagaccgt tcgggacaag
    19921 tatgaaggca taccgaccac ttccatttat tatatgacct gcctgactgt ctttattatc
    19981 tctgttgcat tactgatggt aggtctgtgg aatgcgacat tattactcag cgaaaaaggt
    20041 ttttatggac tggctttctt cttaagcttg tttggtgcag tagcggtgca gaaaaatatt
    20101 cgtgatgccg gaataaaccc accaaaagaa acacaggtta cccaggaaga atacagcgaa
    20161 taactcacgt aagcccggtc agtccaatgt gaccgggctt ttacttaact cactaatctg
    20221 tttctgtcga ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa cgtcgcgcaa
    20281 aaaatccaaa taatatccag tattggccaa tttttaagct caattccccg ggtgcgcagc
    20341 gcatggagaa tcaaggcgtg gaatccgtat atacccaacg aatggcggga gattaagcca
    20401 agtccgcgaa tggtacgcgt atccagcgtg tttttaacca gagtcaatag cgcgattgcg
    20461 cagataaaaa ccatcggccc acagtaaaga taccaggtat cggcaaaatt tccgcgccac
    20521 tgcaattcat ataatgtccc gcgagagata ataaaaactc ccgtcgcaaa cagtgcggcg
    20581 ctcacccacg acagtgcttt atgctgtgtg tccatcatcc ctaaagcgcg gcccaacatg
    20641 ccatacagaa tgtagtaaaa agtatcgcca ttgatatata agttaattgg cagccattca
    20701 aaaccgtcga ttttctgcgg cacggtgttt gggttagcga tgataccaat caccaccatt
    20761 agcaccagca acatttttcc gccgacgttc ttcacctgaa tcagcggtga aaccagataa
    20821 atcaccgcaa tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg cagcaggttt
    20881 ttcagcgcta actccacatt aatggaggta aacagtgcaa tgtagagcag cgcgattgcg
    20941 ctataaaaaa acagacataa gccgatacgc aagaaatggc gcggctgggc gctgcgttcg
    21001 ccaaaaaaga gatagccgga aatcatgaaa aatagcggca cgctgacacg agaggcagag
    21061 ttcagaacat tggcgatatc ccaggtgacg gggctaacac tatgagcatt ggtcacatac
    21121 caggtagtgg tgtgaatcat caccaccatt aaacacgcta tccctcgcag gttatcaatc
    21181 cagtaaattt tgggctgcat ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa
    21241 atcattcagt tggcgcactg gaataatctg agttttaaca ctacagctta tagaggctta
    21301 aggaaattcg taagatatca gccactatac cgatataaat aataagactc acctgcaaac
    21361 cagacggtaa tttaatgatg atgaactctt tctttccggc aatggcgctt atggtgctag
    21421 tgggttgttc tacaccgtca cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc
    21481 gttcgttgaa tatggaagcg ttatgcaagg atcaggcggc aaaacgttat aacaccggcg
    21541 agcaaaaaat cgacgtcacc gccttcgaac agttccaggg aagctatgaa atgcgcggtt
    21601 ataccttccg taaagagcag tttgtctgtt cttttgacgc agatggccat tttttgcatc
    21661 tttccatgcg ttaagccctg ctttttcccg tttcatactg tatatcttcc atccagcggg
    21721 tatactgatc ccttccttta aatccacacg tatccagcac gaaataatat gcaaaagttt
    21781 gataccagga ccttccaggg cttgatcctg accttacagg attactgggc tcgccagggc
    21841 tgcaccattg ttcaaccatt ggacatggaa gtcggcgcgg gaacctctca cccaatgacc
    21901 tgtctgcgcg cgctggggcc agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc
    21961 ccgaccgatg gtcgctacgg cgaaaacccc aaccgtttac agcactacta tcagttccag
    22021 gtggtcatta agccatcgcc ggacaatatt caggagctgt accttggttc tctgaaagag
    22081 ctgggcatgg acccgactat ccacgatatc cgtttcgtgg aagataactg ggaaaacccg
    22141 acgctgggtg cctggggact gggctgggaa gtgtggctaa acggcatgga agtgacgcag
    22201 ttcacttact tccagcaggt tggtggtctg gagtgtaaac cggttaccgg cgagatcacc
    22261 tacggtctgg aacgtctggc catgtacatt cagggcgtag acagcgttta cgacctggtc
    22321 tggagcgacg gcccgctggg taaaaccacc tacggcgacg tgttccatca gaacgaagtg
    22381 gagcagtcca cttacaactt cgaatacgcg gatgtggact tcctgttcac ctgctttgag
    22441 cagtacgaga aagaagcgca gcagctgctg gcgctggaaa atccgctgcc gctgccagct
    22501 tacgagcgta ttctgaaagc cgcccacagc ttcaacctgc tggatgcgcg taaagccatc
    22561 tccgtcaccg agcgtcagcg ctacattctg cgcattcgca ccctgaccaa agcagtggca
    22621 gaagcatatt acgcttcccg tgaagcccta ggcttcccga tgtgcaacaa agataagtaa
    22681 gaggcggcta tgtctgagaa aacttttctg gtggaaatcg gcactgaaga gctgccacca
    22741 aaagcactgc gcagcctggc agagtccttt gctgcgaact ttactgcgga gctggataac
    22801 gcaggcctcg cacacggcac cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa
    22861 gtagctaacc tggcggaagc gcaaccggat cgtgaaatcg aaaaacgcgg cccggcgatt
    22921 gcccaggcgt tcgacgctga aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt
    22981 tgcggtatta ccgttgacca ggctgagcgt ctgactaccg ataaaggcga atggctgctg
    23041 tatcgcgccc atgtgaaggg cgaaagcact gaagcactgc tgccgaatat ggttgcgact
    23101 tcgctggcga aactgccgat tccgaaactg atgcgctggg gtgcaagcga cgtgcacttc
    23161 gtgcgtccgg tgcacaccgt gaccctgctg ctgggcgaca aagtcattcc tgcaaccatt
    23221 ctgggcattc agtccgatcg cgtgattcgc ggccaccgct ttatgggcga gccggaattc
    23281 accatcgata acgccgatca gtatccggaa attctgcgtg agcgtgggaa agtcatcgcc
    23341 gattacgaag aacgtaaggc gaagattaaa gccgatgccg aagaagcagc gcgtaagatt
    23401 ggcggtaacg ctgacttaag cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg
    23461 ccggtcgttc taaccgcgaa atttgaagag aaattcctcg cggtgccgtc tgaagcactg
    23521 gtttacacca tgaaaggtga ccagaagtac ttcccggtgt atgcgaacga cggcaaactg
    23581 ctgccgaact ttatcttcgt tgccaacatc gaatcgaaag atccgcagca aattatctct
    23641 ggtaacgaga aagtcgttcg tccacgtctg gctgatgccg agttcttctt caacaccgac
    23701 cgtaaaaaac gtctggaaga taacctgccg cgcctgcaaa ccgtgctgtt ccagcaacag
    23761 ctgggtacgc tgcgtgacaa gactgaccgc atccaggcgc tggctggctg gatcgccgaa
    23821 cagattggcg ctgacgttaa ccacgcaacc cgtgcgggcc tgctgtctaa gtgtgacctg
    23881 atgaccaaca tggtcttcga gttcaccgac acccaaggcg ttatggggat gcactacgcg
    23941 cgtcacgatg gcgaagcgga agacgtagcc gtcgcgctga atgagcagta tcagccgcgc
    24001 tttgccggtg atgacctgcc gtccaacccg gtagcttgtg cgctggcgat tgctgataag
    24061 atggatactc tggcgggtat cttcggtatc ggtcagcatc cgaaaggcga caaagacccg
    24121 tttgcgctgc gtcgtgccgc acttggcgtg ctgcgaatta tcgttgagaa gaacctcaac
    24181 cttgatctgc aaacgctgac cgaagaagcg gtgcgtctgt atggcgataa gctgactaat
    24241 gccaacgtag ttgatgatgt tatcgacttt atgctcggtc gcttccgcgc ctggtatcag
    24301 gacgaaggtt ataccgttga caccatccag gcggtactgg cgcgtcgtcc gactcgtccg
    24361 gctgatttcg atgcccgcat gaaagcggta tcgcacttcc gtaccctgga agcagctgca
    24421 gcactggcgg cggcgaacaa acgtgtatct aacatcctgg cgaaatctga cgaagtgctg
    24481 agcgatcgcg tgaatgcctc tactctgaaa gagccggaag aaattaaact ggcgatgcag
    24541 gttgtggtgc tacgtgacaa gctggagccg tactttgcgg aaggtcgtta ccaggatgcg
    24601 ctggtcgaac tggcagagct gcgtgaacca gtcgatgcct tcttcgataa agtgatggtc
    24661 atggttgatg acaaagaatt gcgtatcaac cgtctgacca tgctggagaa actgcgcgaa
    24721 ctgttcctgc gcgttgcgga tatttcgctg ttgcagtaat aacgccgtta ttaaaaagcc
    24781 tgccatctgg caggcttttt ttatcgctaa ataatacagc aacctttaat aatcttctgc
    24841 tgaataaaga ttatctcata tattaatttt atgagatttt tttaggatta tatcaaggag
    24901 aagaaacaaa cttattaagc tagaatagcc acgggtgctt gagactgttt gtctcaggta
    24961 ttcaccgaaa ggcagacaga gaaaagcccc acctgactat aaatcaaagt gaggcccctc
    25021 tatgtataga caccataagg ttagcctctt actcgttgga aatcaacaca gggggctggg
    25081 aatgccgcag aaatatagat tgctttcttt aatagtgatt tgtttcacgc ttttattttt
    25141 cacctggatg ataagagatt cactgtgtga attgcatatt aaacagggga gttatgagct
    25201 ggcggcattt ttagcctgca atttgaaaga gtaagagtct tcggcggaaa gttattcccg
    25261 ccttt
//
LOCUS       DAZGXS010000066        24893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGXS010000066 DAZGXS010000000
VERSION     DAZGXS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            156..1031
                     /locus_tag="ACHKG5_002734"
     CDS             156..1031
                     /locus_tag="ACHKG5_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0868813.1"
                     /translation="MTKPYGFQALEQPLASLPDTLRQLILERIQNLTHYEPVIGIMGK
                     SGAGKSSLCNELFRGEVSPVSDVKVCTREVLRFRLRSGRHSLMIVDLPGVGENGQRDH
                     EYRALYRRMLPELDLVLWVIKADDRALSVDEQFWRGVMQPYQQQVLFVLNQADKIEPC
                     HEWDIRTGTPSPQQQANLKAKQAAITTIFTPRHPVCMVSALTGWGIEEMVSTIMRCLP
                     DRATSPVATQLHGRFCTEPVRSQARDGFGEAVGRVFDTAESSLLLPAPLKMVIRTVRD
                     AVVSVARAVWDWIFF"
     gene            1245..1952
                     /locus_tag="ACHKG5_002735"
     CDS             1245..1952
                     /locus_tag="ACHKG5_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFP0868814.1"
                     /translation="MTQAEHRHDRLAVRLSIIISRLVAGETLDIRKLAAEFDVSVRTL
                     RRDFRERLMYLDLEYRQGQCRLLSGSRQRELAVITFARQSGVESLFPGLDGHLVSSLL
                     GGPGDSPCLIWQGDSAQTVTDSGVFIRLVNAVQEYRKVTLHGNGCNHTDISPYRLVLH
                     AGSWYLTGAHQTRIIALPLTDIRCVTLHPDTFTPDATVRDILSRPKFLQALPHFRFIG
                     EVLSGLCPEPHQPATEG"
     gene            1954..2103
                     /locus_tag="ACHKG5_002736"
     CDS             1954..2103
                     /locus_tag="ACHKG5_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868815.1"
                     /translation="MMKIRRITPGNPTSGKRCPDCGSHDTIPWADGWMCRDCGCEWGK
                     DNGYL"
     gene            2115..2330
                     /locus_tag="ACHKG5_002737"
     CDS             2115..2330
                     /locus_tag="ACHKG5_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0868816.1"
                     /translation="MGFWSVTGKLLKEVAKDALNSAQEEKTLHDRLETRSSEELKRII
                     TDNGIFSSVTEMEKRLARKVLRDRDER"
     gene            2422..2832
                     /locus_tag="ACHKG5_002738"
     CDS             2422..2832
                     /locus_tag="ACHKG5_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868817.1"
                     /translation="MKAFILSLIVSGLLLSASVNATEVKLPRTVIDVKDGSEQMCGSE
                     DVKDGICSLIGVSDDGKYALLSSPGGSACYDPYIIVVNLATSKGKMMDYNRHATCSGE
                     ATAKFVRSSDKDHLTVVIYDPSIKKNVGLNTIDF"
     gene            3006..3836
                     /locus_tag="ACHKG5_002739"
     CDS             3006..3836
                     /locus_tag="ACHKG5_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868818.1"
                     /translation="MRCDPDAGDNENGYGSCPFLGSIYDADPVFKKDLDDALKSAGLT
                     GLTGKQGSMNGPDSGLIPVVVGDEKWLLGSVCERGNCGDHYLKILYIPSEHVIAGFYY
                     NSGEENMFGDVGDAEARLLRRDVPDVTPVQQAPALPVTHTDGAPADTIWTFKGDSSKT
                     LWQVCGGQSSSCAIIANTKYYVAVLNRKSATGCAFGDFYVAARDTATWRQYDTGTCSA
                     DAYIRKGSINNGQYLSVDIGINGVLVKQFPIGYWSMQKEFSGNRRPSWSKVKEKNQQH
                     "
     gene            3926..4744
                     /locus_tag="ACHKG5_002740"
     CDS             3926..4744
                     /locus_tag="ACHKG5_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0868819.1"
                     /translation="MRLASRFERINQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     SYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGLSGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4836..5321
                     /locus_tag="ACHKG5_002741"
     CDS             4836..5321
                     /locus_tag="ACHKG5_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0868820.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLISQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
     gene            5337..5813
                     /locus_tag="ACHKG5_002742"
     CDS             5337..5813
                     /locus_tag="ACHKG5_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0868821.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5882..6103
                     /locus_tag="ACHKG5_002743"
     CDS             5882..6103
                     /locus_tag="ACHKG5_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0868822.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAKAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6183..6477
                     /locus_tag="ACHKG5_002744"
                     /pseudo
     CDS             6183..6477
                     /locus_tag="ACHKG5_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            6680..7001
                     /locus_tag="ACHKG5_002745"
                     /pseudo
     CDS             6680..7001
                     /locus_tag="ACHKG5_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246873.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
     gene            7387..8457
                     /locus_tag="ACHKG5_002746"
     CDS             7387..8457
                     /locus_tag="ACHKG5_002746"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP0868823.1"
                     /translation="MLQYTSVEICAGAGGQALGLHNAGFTHRALVEIDPAACETLRLN
                     NELHSLGWENIIEGCVKHFAEHTAYNFSDIDLVAGGVPCPPFSKAGKQLGKDDERDLF
                     PTALKIVNIIKPKAVMIENVSGLLDPKFKQYRQELDHAFSAMGYKTFWKLHNASDYGV
                     PQLRPRVLLVALRGKYSAHFRWPSETLIPPTVGDALFDLMSQGGWEGAEAWRKAANNI
                     APTLVGGSHKHGGPDLGPTRAKKAWQALGVNGHSIADSSPEKGFTGYIGRDGNIRAGL
                     ENMPRLTVRMAARIQGFPDWWEFSGKKTAAYRQVGNAFPPPVAEATGRQIRKALEAVD
                     LMKKKQLASPLESNDVQSLRIA"
     gene            complement(8436..9095)
                     /locus_tag="ACHKG5_002747"
     CDS             complement(8436..9095)
                     /locus_tag="ACHKG5_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0868824.1"
                     /translation="MELFENYLSALPKKKRLSKVKLIILKRLWVSDDFDFPKPWVPST
                     ELLELTGQKYFDRRTRELRDELGCDLESSYIPEFSGHAWRLKSSMIAPPMEREYLTES
                     QRSSLFSNCSNTCATCGKTVAAGVRGLQADHKIPLSRGGGNELTNWQALCHNCNVGKR
                     RACEGCTEDCYTCSWAFPEKFGITTMLHLPEASLRRAQQLADAKGFSLDEIVDYAIRK
                     L"
     gene            complement(9425..9500)
                     /locus_tag="ACHKG5_002748"
     tRNA            complement(9425..9500)
                     /locus_tag="ACHKG5_002748"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9465..9467),aa:Thr,seq:cgt)
     gene            complement(9615..10868)
                     /gene="proA"
                     /locus_tag="ACHKG5_002749"
     CDS             complement(9615..10868)
                     /gene="proA"
                     /locus_tag="ACHKG5_002749"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0868825.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10880..11983)
                     /gene="proB"
                     /locus_tag="ACHKG5_002750"
     CDS             complement(10880..11983)
                     /gene="proB"
                     /locus_tag="ACHKG5_002750"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0868826.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12271..13326
                     /gene="phoE"
                     /locus_tag="ACHKG5_002751"
     CDS             12271..13326
                     /gene="phoE"
                     /locus_tag="ACHKG5_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0868827.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(13365..13766)
                     /gene="crl"
                     /locus_tag="ACHKG5_002752"
     CDS             complement(13365..13766)
                     /gene="crl"
                     /locus_tag="ACHKG5_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0868828.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(13824..15068)
                     /gene="frsA"
                     /locus_tag="ACHKG5_002753"
     CDS             complement(13824..15068)
                     /gene="frsA"
                     /locus_tag="ACHKG5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0868829.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(15160..15618)
                     /gene="gpt"
                     /locus_tag="ACHKG5_002754"
     CDS             complement(15160..15618)
                     /gene="gpt"
                     /locus_tag="ACHKG5_002754"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0868830.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            15879..17336
                     /gene="pepD"
                     /locus_tag="ACHKG5_002755"
     CDS             15879..17336
                     /gene="pepD"
                     /locus_tag="ACHKG5_002755"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0868831.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(17393..17914)
                     /gene="prfH"
                     /locus_tag="ACHKG5_002756"
                     /pseudo
     CDS             complement(17393..17914)
                     /gene="prfH"
                     /locus_tag="ACHKG5_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(17913..18116)
                     /locus_tag="ACHKG5_002757"
                     /pseudo
     CDS             complement(17913..18116)
                     /locus_tag="ACHKG5_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(18435..18887)
                     /gene="yafP"
                     /locus_tag="ACHKG5_002758"
     CDS             complement(18435..18887)
                     /gene="yafP"
                     /locus_tag="ACHKG5_002758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0868832.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(18897..19295)
                     /gene="yafO"
                     /locus_tag="ACHKG5_002759"
     CDS             complement(18897..19295)
                     /gene="yafO"
                     /locus_tag="ACHKG5_002759"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFP0868833.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(19298..19591)
                     /gene="yafN"
                     /locus_tag="ACHKG5_002760"
     CDS             complement(19298..19591)
                     /gene="yafN"
                     /locus_tag="ACHKG5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFP0868834.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(19643..20698)
                     /gene="dinB"
                     /locus_tag="ACHKG5_002761"
     CDS             complement(19643..20698)
                     /gene="dinB"
                     /locus_tag="ACHKG5_002761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0868835.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(20769..21554)
                     /gene="lafU"
                     /locus_tag="ACHKG5_002762"
     CDS             complement(20769..21554)
                     /gene="lafU"
                     /locus_tag="ACHKG5_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP0868836.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            21526..23238
                     /locus_tag="ACHKG5_002763"
                     /pseudo
     CDS             21526..23238
                     /locus_tag="ACHKG5_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(23383..23880)
                     /gene="rayT"
                     /locus_tag="ACHKG5_002764"
                     /pseudo
     CDS             complement(23383..23880)
                     /gene="rayT"
                     /locus_tag="ACHKG5_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(24056..24805)
                     /gene="yafL"
                     /locus_tag="ACHKG5_002765"
     CDS             complement(24056..24805)
                     /gene="yafL"
                     /locus_tag="ACHKG5_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0868837.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         6154 a         6391 c         6246 g         6102 t
ORIGIN      
        1 cttctcaggg tacctacacc ccacgcgtgg tccgaagccc cctcccaggg gataattgca
       61 ttaaaaatta ttttagaaag atattatctc cgtgaatccc ccgcctccct taccggagtg
      121 cggggatttt tgttttttca tgcagagatc cctttatgac taaaccatac ggctttcagg
      181 cccttgaaca accgctggcg tcgcttcccg atacgcttcg ccagcttatt cttgaacgta
      241 ttcagaatct cacccactac gagccggtga ttggtatcat gggcaaaagt ggtgccggta
      301 agtcttcgct ctgtaacgaa ctcttccggg gtgaggtctc ccccgtcagt gatgtgaaag
      361 tctgtacccg tgaagtgctg cgcttccggc tacgcagcgg gcgtcacagt ctgatgattg
      421 ttgatttacc gggtgtggga gaaaacgggc aacgggacca tgaataccgt gccctgtacc
      481 ggcgtatgct gccggaactg gacctggtgc tgtgggtcat taaggccgat gaccgggcgc
      541 tgtcggtgga cgagcagttc tggcgcggtg tgatgcagcc ttaccagcag caggtgctgt
      601 ttgtgctgaa tcaggccgac aaaatcgaac cctgccatga atgggatatc cgtaccggca
      661 ccccctcgcc acagcagcag gctaacctga aggcgaagca ggccgccatt accacgatat
      721 tcactccccg tcatccggtc tgcatggtgt cagcattgac cggctggggc attgaagaga
      781 tggtgtcaac cataatgcgc tgtctgcctg accgcgccac cagtccggtg gcgacgcagt
      841 tacacgggag gttctgtacg gagccggtca ggtcgcaggc ccgtgacggc tttggcgaag
      901 cggtggggcg ggtgttcgat acggcggagt cttcgttgct cctgcccgcc ccgctgaaaa
      961 tggtgattcg gaccgtgcgt gatgctgtgg tatcggttgc acgggctgtc tgggactgga
     1021 ttttcttctg ataccgagaa ggcattttgc tgacgttcgg acacatcctg tccgtttgca
     1081 ggcctttgcc cgaagaaaaa agagcaacat cccccgcagc tcccgtcatt cctgaaactg
     1141 agcattttta aaaatcgtct ttctgtgtcc ataccctgcc tctcgtcctc gttaatataa
     1201 ccctcatctt ttcattgaat tagcttaccg gagaacgaag ttttatgact caggctgagc
     1261 atcgccatga ccggcttgcc gtcagactgt cgataattat tagccgtctg gtggcaggcg
     1321 aaacgctgga tatacgcaaa ctggctgctg aatttgacgt gtcagttcgt accctgcgcc
     1381 gggattttcg tgaacgactg atgtacctgg acctggagta tcgacagggc cagtgccgtc
     1441 tgctgtccgg cagccgtcag cgggaactgg cagtgataac ctttgcccgt cagtcgggcg
     1501 ttgagtccct gtttccggga cttgacggac atctggtcag ttcgcttctg ggtgggccgg
     1561 gtgattctcc ctgccttatc tggcagggag attctgcaca gaccgtcacc gacagtggag
     1621 tctttatccg gctggtaaat gctgtccagg aataccggaa agtgactctg cacggcaacg
     1681 gatgcaacca tacggatatc tcaccctacc gcctggttct gcatgccggt agctggtatc
     1741 tgacaggggc acaccagaca cgcatcatcg cactgccact gactgatatc cggtgcgtca
     1801 cgcttcatcc ggacactttt acacccgacg ccacggtgcg cgacatcctt tctcgcccga
     1861 aatttttaca ggcattacct catttccggt tcataggtga agtgctgtcc ggcttatgcc
     1921 ctgaaccaca tcaacccgca acagaaggat aacatgatga aaataagacg tatcacaccc
     1981 ggtaacccga cctccggcaa acgctgcccg gactgtggct cccacgacac cattccctgg
     2041 gccgatggct ggatgtgccg ggactgcggg tgtgagtggg gcaaagataa cggttatctg
     2101 taaaggtggc tcccatggga ttctggagtg taaccgggaa actgcttaaa gaggttgcaa
     2161 aagacgccct caacagtgca caggaggaaa aaactcttca tgaccgactg gagaccagga
     2221 gcagcgaaga gctaaagaga attatcaccg acaacggtat tttcagcagt gtaacggaga
     2281 tggaaaaacg tctggcgcga aaggttttac gggaccggga cgaacgttaa ttattccaca
     2341 gggccgacaa caggccctgc ttgtggttca tgagcagaat aaaaggaata tatcccccgt
     2401 aatgataaac aggatgagta tatgaaagcg tttatattgt cattaatagt ttcaggtttg
     2461 ttgttgagtg catcagttaa tgctacagag gttaaattac cccgtactgt gattgatgtg
     2521 aaagatggaa gtgagcagat gtgcggaagt gaggatgtaa aagacgggat atgcagcctc
     2581 attggtgtct ctgatgatgg caaatatgcc ttgctgagca gtccgggagg ttcggcctgt
     2641 tatgatcctt atatcatcgt tgtcaatctt gctaccagca aaggtaaaat gatggattat
     2701 aaccgtcatg caacatgcag tggtgaggcc accgctaagt ttgtccgttc ttcagacaaa
     2761 gaccatctta ccgttgttat ttacgatccc tccatcaaaa agaatgtcgg tcttaatacc
     2821 attgactttt aaatttttta cagggctctt cacggagccc gtttctttta tataaacagg
     2881 atggagtaaa aataatcatg tttcttgtat tacgtaaatt aaaacagata atactgatat
     2941 cctcgctttg ttttatttcc accggagttt acgctgccgg tgtcgcaaaa acggcagcag
     3001 atgcaatgcg ctgtgatcct gatgctggtg ataatgaaaa cggatacggt tcctgcccct
     3061 ttctgggcag tatctacgat gctgacccgg tattcaagaa agaccttgat gatgcgctga
     3121 agtcagcagg actcaccggg ttaacgggaa aacagggctc catgaacggc cctgacagtg
     3181 gattaattcc ggtggtcgtt ggtgacgaga agtggctgct ggggtctgtc tgtgagcggg
     3241 gtaattgcgg tgaccattat ctgaaaatac tgtatatccc ctcagaacat gttattgccg
     3301 gtttttatta caacagcgga gaggaaaaca tgtttggcga tgtcggggat gctgaagcac
     3361 gactattacg ccgcgacgta cccgacgtta caccagtgca gcaggctccg gcattgccag
     3421 taacacatac agacggcgca ccggcagaca ctatctggac ctttaagggt gacagcagca
     3481 aaacgttatg gcaggtctgc ggcggtcaga gcagcagttg cgccattata gccaacacta
     3541 agtattacgt tgccgttctg aatcgtaagt cagcaacggg ctgtgctttc ggtgattttt
     3601 atgttgccgc ccgcgatacc gcaacatggc ggcagtatga taccggcacc tgcagtgcgg
     3661 atgcgtacat ccgcaagggc agcatcaaca acggtcagta tctgtccgtc gatattggta
     3721 tcaacggggt gctggtgaag cagttcccga ttggttactg gtcaatgcag aaggaatttt
     3781 ccggcaaccg acgcccctca tggtcaaaag ttaaagagaa aaatcagcaa cattaatcat
     3841 cagcaataat atattccatc aggccatgtt cctccgggga catggccttt ttgcttttat
     3901 ttcattatca gataaggagt taattatgcg attagccagt cgcttcgaac ggattaatca
     3961 gatacgccgt gaccgcccac tgacccatga agaactgatg cactatgttc ccagcatttt
     4021 tggggaagac cggcatacct cccgcagtga acggtattcg tacatcccca ccatcacagt
     4081 gctggaaagc ctgcagcggg aaggctttca gccattcttc gcctgccaga cccgcgtacg
     4141 tgacccgggc cgccgggggt ataccaaaca catgctgcgt ctgcgccgtg acggggagat
     4201 aaacggacaa catgtccctg aaattattct gctcaactcc catgacggta cctccagcta
     4261 ccagatgctg ccgggttact tcagattcgt ctgccagaac ggctgcgtct gtggtcagtc
     4321 tctgggggaa gtgcgtgttc cgcaccgggg aaatgtggtg gacagagtca ttgaaggggc
     4381 ttacgaggtg gtgggtgtgt ttgaccggat agaggagaag cgtgatgcca tgcagtcgct
     4441 ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg gcactgactt accgttatgg
     4501 tgacgaacat cagcccgtca ccaccgccga cattctgaca ccacgacgca gggaggatta
     4561 cggtaaggac ctgtggagtg cttatcagac catccaggag aatatgctga aaggcgggct
     4621 ttccggtcgc agtgcaaaag gaaaacgtat ccacacccgt gccattcaca gcatcgatac
     4681 cgacattaag ctcaaccggg cattgtgggt gatggcagaa acgctgctgg agagcatgcg
     4741 ctgatgcctt ttccctgaaa gcgcagtcct gactcacggc tgtcccttcc cccgggcatt
     4801 cttccattca tttacttttt ataaggaata atctcatgac aaccgttacg cataattcca
     4861 ccacaccttc tgtttccgta accgctgcat cagggaataa cccacctcag ctggttgcta
     4921 ctctcgtccc tgatgaacag cgcatcagct tctggccgca acattttggc ctcatttcac
     4981 agtgggtgac cctggagccc cgtatcttcg gctggatgga ccgtctttgc gaagactact
     5041 gtggtggtat ctggaatctg tacaccctga acaacggcgg ggcatttatg gctccagaac
     5101 cggatgaaga tgatggagaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg
     5161 aaatgagccc ggaagccgcc ggtattgccg cctgtctgat gacgtacagc catcatgcct
     5221 gtcgtacgga gtgttatgcc atgacggtcc attattaccg gctgcgggat tacgccctgc
     5281 agcatccgga atgcaacgcc attatgcgca tcatcgactg aaagggggcc ggaacaatgc
     5341 agcagctttc ctttctgccc ggagaaatga caaccagaga gcgcagcctc attctgcggg
     5401 ccctgaaaac cctggaccgc catcttcatg aacccggcgt ggccttcacc tccacccgtg
     5461 cggcacggga atggctgatt ctgaacatgg cgggactgga gcgtgaagaa ttccgggtgc
     5521 tgtatctgaa caaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca
     5581 accgcacgga agtccatccc cgggaagtga ttaaacgcgc cctgtaccac aatgccgcag
     5641 ccgtggtgct ggcacacaat cacccgtccg gtgaagtcac acccagcaag gcagaccgcc
     5701 ttatcacgga acgtctggta caggcactgg gcctggtgga tatccgggtg ccggaccatc
     5761 tgatagttgg tggcaaccag gttttctcct ttgccgaaca tggtctgctt taacccgtca
     5821 ccgtcaccac cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacac
     5881 catgaaaatc atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg
     5941 tctttttccg ttctgtaccg gcaaataccg ctggcacggc agtgctaagg cgtataccgg
     6001 tcgtgaagtg cagaatattc ccggtgtgct ggccgtgttt gcagaacgcc gtaaggacag
     6061 ttttggcccg tatgtccggc tgatgagcgt cactctgaac tgagtcagta agggcattct
     6121 gatgagcaga attatcgaca ttacctgacc gttcttaacc gattttctgt gaggatttta
     6181 tcgtgtcaga cacgctccat gaaacaaatt atcccgacga caatcacgac cgcccctggt
     6241 gggggctgcc ctgcaccgtt cgtgacagtg acaccccgtt ttggcgcccg tctggtgcag
     6301 gagggcaatc agttgcatta ccttgcagac cgcgccggta tcagaggccg gttcagcgat
     6361 gcggatgcat accatctgga ccaagccttt ccactgctga tgaaacaact ggaactcatg
     6421 ttcaccagcg gtgaactgaa tccccgtcat cagcagaccg tcacgctgta tgcgaaaagg
     6481 atgatcggtg aatcccccct tcggttcgca ctggtacatt tgccagcgat gaaaaagcta
     6541 ggactgcatt atgccatacc gaccgacata acaatacgga gcagggcata aaccaagata
     6601 cgctggctgc catacgacag tgacgtaccg cgaaaaaccc gtccgcaatt aatttctttt
     6661 attcgcagaa taaccgacga tgagaatttt acctatattc cgaaaacgtg tggcgaagtc
     6721 ctctgtaacc gtctgacagc ccctagttac atacctgcag gacaaacact acagcctgac
     6781 tcttaacgat acgcagcgca ttgacgacac tgttatgaag gtacatattg aaaacgggat
     6841 tccccttagc cgatacagtc aacttcctag tagataagta cgaactgatt cgtatcgatc
     6901 acagagtgtt gagcaggcgg gagcaatcac attatcttca gaccgtagat atcttacgag
     6961 cacgacaaac aaccgacatg ttgcgacgaa gccaaagctg aattccacaa gcagcgtgcc
     7021 aatctttctt attcatttgt tctctgcaga atgcgtctgc tatttacagc ggacgcaatg
     7081 catcttacgg acaaaaaatc atgtaaacag atagtagttc ttttcgtact cagctaaggc
     7141 atattattca gtccacatga gattctgcct gtctgatcaa gagaatgcca atagtactgt
     7201 cgaatgttag ttatttgata aatataggat tccaaaacaa agttgtagat tgccttgtca
     7261 ttcagcaatc aagtaactaa gctgaccggt tcaatagcga cggtactact tgttttgtac
     7321 aatttgttgc gtaatggtct ctgatcagat aaacatacaa ctgtttttat atacaggttt
     7381 aatgacatgt tacaatacac atctgtagag atttgtgcag gggcaggagg tcaagctcta
     7441 gggttacata acgctggctt cactcaccgc gcgctcgtcg agattgatcc tgctgcctgc
     7501 gaaactcttc gcctcaacaa tgagctccat tctttaggtt gggaaaatat catagagggt
     7561 tgtgttaagc attttgctga acatactgca tataattttt ctgatattga cttggtcgca
     7621 gggggagtgc cctgcccgcc attctcaaaa gcagggaaac aactaggcaa agatgatgaa
     7681 cgtgatttgt ttccaacagc gctcaaaatt gtgaacatca ttaagccaaa agcagtaatg
     7741 attgaaaatg tttcaggttt attagaccca aagtttaaac aataccgaca agaactagat
     7801 catgctttct ctgcaatggg ttataaaacc ttttggaaac ttcataacgc ttctgattac
     7861 ggagttcctc agcttcgtcc ccgagttctt ttagttgcat tgcgtggtaa gtattccgct
     7921 cattttcgtt ggccgagtga aacgttgata ccaccgactg tgggtgatgc tctctttgac
     7981 ttaatgtcac agggtggctg ggaaggagcc gaggcatgga gaaaggccgc taacaatata
     8041 gcccctacac ttgttggcgg ttcccataag catggaggtc cagaccttgg accgacgaga
     8101 gccaaaaaag cttggcaagc gcttggagtc aacgggcatt ccatagctga tagtagccct
     8161 gaaaaggggt ttactgggta tattggaaga gacggaaata ttcgagcagg tcttgaaaat
     8221 atgcctcgtt tgacggtgag aatggctgca cgaatccaag gtttcccgga ctggtgggaa
     8281 ttctctggga aaaaaacagc tgcttatcgt caagtaggta acgcttttcc tcctcctgtt
     8341 gccgaagcaa caggaaggca aattcgtaaa gcactcgaag cggttgatct aatgaaaaaa
     8401 aaacagttgg catcaccact ggaatcgaat gatgttcaaa gcttgcgtat tgcgtagtca
     8461 acgatttcgt ctaaagaaaa accctttgca tcagctagtt gttgggctct tcttaaacta
     8521 gcttctggca gatggagcat tgttgttatc ccaaattttt caggaaatgc ccatgagcat
     8581 gtgtaacaat cttcagtaca tccctcacat gctcttctct tacctacgtt gcaattgtgg
     8641 caaagagctt gccaattggt taactcattt ccaccaccgc gtgacaaagg tattttgtga
     8701 tccgcttgta gccccctgac cccagcggca acagttttac cacatgttgc acatgtattg
     8761 ctgcagttag aaaataaact ggatcgttgt gattctgtca gatattccct ttccattggc
     8821 ggggcaatca tgcttgattt cagccgccaa gcatgtcccg agaattccgg aatgtacgag
     8881 ctttcgagat cacagcctag ttcatcacgc aactctcttg ttcgtctatc gaagtacttt
     8941 tgccctgtca attccagtaa ttctgtcgat gggacccaag gtttaggaaa atcaaaatca
     9001 tctgaaaccc ataatctctt taggataatc aacttgactt tactcaatcg ctttttttta
     9061 ggtaacgctg acaaataatt ttcgaataat tccatactat ctctcgaaag aaaccttaat
     9121 ctcgcaaggg attacaccat agatggaagt agacaggaaa gggctatcta aaagggcagg
     9181 catatagagc aataatcaat gaaacaacct gtacaggcct gctaagctgc ccccccaaat
     9241 gtccaacatc aatatcagaa atattgtttc atcaagatga aaaaatcaac aagactgagc
     9301 caatctgaga tacggatgct ctattgttga tttatgttga cgaaaattac ttacacatgg
     9361 agttacacgg aaaaaatcat gatgcagaca aagagaggat ttaatcgtaa ttgcttgata
     9421 taaatggtgc cgataatagg agtcgaacct acgaccttcg cattacgaat gcgctgctct
     9481 accaactgag ctatatcggc cctgaaagga catgttcacg aacgtgaatc acggtggaca
     9541 aggttaaaac taaccgggcg atgcgtcaat ggccttgtga atcaaacggc tacttttgca
     9601 tcacccggtt ttatttacgc acgaatggtg taatcaccaa tgccgatcca cttgtaagtg
     9661 gtcagtgctt ccagccccat tgggccacgc gcgtggagtt tttgtgtgct taccgccact
     9721 tccgcaccca gtccaaactg gccgccgtcg gtaaaacgcg tagaggcgtt aacgtaaaca
     9781 gcggacgaat ccacttcgtt aacaaaacgc tgggcgttgc gcatatcgcg ggtcaggatc
     9841 gcatcggagt gttgcgtgcc gtgttcacga atatgggcga tggcatcgtc aagatcgctg
     9901 acgattttga cgttcaaatc taatgacaga aactcatcgt catactcttc ggctttaacc
     9961 gccaccacct tcgcagggcc tgtctgcaac tgcgccagcg cagatgcatc tgcgtgtaac
    10021 gtcacgccgc tttccgccat ttgtttgctt aatgcgggca ggaagctata ggcgatgttt
    10081 ttattcacca gcaacgtttc taccgtatta catgtgctcg gacgctgagt tttcgcattg
    10141 acgattactt tcagggcttc agcaatctct gcactttcat caacataaat atggcatacg
    10201 cctataccac ctgtgatcac cgggatcgtc gactgctcgc ggcacagttt atgcaaacca
    10261 gccccgccgc gcgggatcag catgtcgatg tatttatcca tacgcagcat ttcactgacc
    10321 agcgcacggt caggattatc aatcgcctgc acggcacccg ccggtaagcc acaggatttc
    10381 agggcgtcct gaatcaccac caccgttgcc gcgttagtgc gacaggtttc tttgccacca
    10441 cgcaggatca ccgcgttacc ggttttcagg cacagcgaag cgacatcaac cgtcacgttc
    10501 gggcgcgctt cataaatcac gccaataacc cccagcggta cgcgacgacg ctcaagacgc
    10561 aggccgctgt ccagtacgcc gccatcgatt acctgcccca ctggatcggc gaggttgcac
    10621 acctgacgta catcgtcggc aatgcctttc agccgtgcgg gcgtcagtgc cagacggtca
    10681 agcatcgctt cgctaaggcc attggctcgc gcgtcagcaa catcctgggc gttagcgttg
    10741 aggatgattt cgctttgtgc ttccagttca tcggcgattt tttccagcac gcgatttttt
    10801 tcgcggctgg agagttgcgc taatttatac gaggcttgct tcgcggcaat gcccatttgt
    10861 tccagcatca gcctgctcct taacgggtga tcatgtcatc acggtgaacg gcaaccgggc
    10921 cgtattcata tcccagtatt gcatcaattt cttgcgagtg gtgtccggca atacggcgta
    10981 atgcatcgct gttgtaacga ctgacgccgt gggcgatatc gcgaccttcg aggttacaaa
    11041 tgcggatgac ttcaccacgc gagaagttgc cagtcacgct tttaatgcct ttcggcaaca
    11101 gggagctgcc gcgttcaaga atggcggcgg ttgccccttc atctaccgtg atttcacccg
    11161 caggcggcgc accgaaaatc cagcgtttac ggttttcaag cggagtcgcc tgggcatgga
    11221 acagcgtacc gacggaaatg ccttccatca catcaccaat aacgcccggc ttgctgcccg
    11281 cggcaataat ggtgtcgata cccgcacggc aagccacgtc agcggcctgc aatttggtac
    11341 tcatgccgcc agttccgagg cctgaaacgc tgtcaccagc aatcgcgcgc agtgcgtcat
    11401 caatgccgta aacatcttta atcagttctg cctgcggatt gctgcgcggg tcagcggtat
    11461 acaaaccttt ttgatcggtc agtaacaaca gtttatcggc acccgccaga atcgccgcca
    11521 gtgcagaaag gttgtcgtta tcgccgactt taatctctgc cgtagcgaca gcatcgttct
    11581 cattgattac cggaacgata ttgttatcga gcaacgcacg cagggtgtcg cgggcgttca
    11641 ggaagcgttc acggtcttcc atatcagcac gagtcagcag catttgcccg acgtgaatgc
    11701 cataaatcga aaacagctgt tcccacagtt gaatcagtcg actctgccct accgccgcca
    11761 gcagttgttt cgaggcgata gtcgctggca gttccgggta acccaggtgc tcacgtccgg
    11821 cggcgatcgc gcccgacgtc acaataacaa tccgatgccc ggcggcatgt aactgcgcgc
    11881 actggcgaac aagttcaacg atatgggcac ggttcaggcg gcgcgatccg cctgttagca
    11941 cactggtgcc gagttttacc accagcgtct ggctgtcact catgattctc tgccattcaa
    12001 ttttaggaaa aatgatatca aacgaacgtt ttagcaggac tgtcgtcggt tgccaaccat
    12061 ctgcgagcaa agcatggcgt tttgttgcgc gggatcagca agcctagcgg cagttgttta
    12121 cgcttttatt acagatttaa taaattacca cattttaaga atatcattaa tctgtaatat
    12181 atctttaaca atctcaggtt aaaaactttc ctgttttcaa cggggctctc ccgcagaata
    12241 ttcgcgcgtt aattaaaatc aggaatgaaa atgaaaaaga gcactctggc attagtggtg
    12301 atgggcattg tggcatctgc atccgtacag gccgcagaaa tatataacaa agacggtaat
    12361 aaactggatg tctatggcaa agttaaagcc atgcattata tgagtgataa cgacagtaaa
    12421 gatggagacc agagttatat ccgttttggt tttaaaggcg aaacacaaat taacgatcaa
    12481 ctgactggtt atggtcgttg ggaagcggag tttgccggaa ataaagcgga gagtgatact
    12541 gcacagcaaa aaacgcgtct cgcttttgcc ggattgaagt ataaagattt gggttctttc
    12601 gactatggcc gtaacctggg cgcgttgtat gacgtggaag cctggaccga tatgttcccg
    12661 gaatttggtg gcgactcctc ggcgcagacc gacaacttta tgaccaaacg cgccagcggt
    12721 ctggcgacgt atcggaacac cgacttcttc ggcgttatcg atggcctgaa cttaaccctg
    12781 caatatcaag ggaaaaacga aaaccgcgac gttaaaaagc aaaacggcga tggcttcggc
    12841 acgtcattga catatgactt tggcggcagc gatttcgcca ttagtggggc ctataccaac
    12901 tcagatcgca ccaacgagca gaacctgcaa agccgtggca caggcaagcg tgcagaagct
    12961 tgggctacag gtctgaaata cgatgccaat aatatttatc tggcaacttt ttattctgaa
    13021 acacgcaaaa tgacgccaat aactggcggc tttgccaata agacacagaa ctttgaagcg
    13081 gtcgctcaat accagtttga ctttggtctg cgtccatcgc tgggttatgt cttatcgaaa
    13141 gggaaagata ttgaaggtat cggtgatgaa gatctggtca attatatcga tgtcggggct
    13201 acatattatt tcaacaaaaa tatgtcagcg tttgttgatt ataaaatcaa ccaactggat
    13261 agcgataaca aattgaatat taataatgat gatattgtcg cggttggcat gacctatcag
    13321 ttttaatgat tattgccgga tgtgatgcat ccggcacatt tcactcacgc cgttaacttc
    13381 accggctcgt cacgaaaatc atccgccggt tccaacttca gattcagcgt cgtcagcagt
    13441 tcacgcagct tctcgtgaaa ctcacgcagg gtgtgctcca gtcgttcaac cacttcagta
    13501 tcttttaccg gaacactctt ccagtcgcct gctttatcga acagaccaaa ctggtaactg
    13561 taggtaaaac gggattcctg cgcttcaagc tccatccacc agccccagaa ctcacgcact
    13621 tccggtgccg gtttcacgtt gacgcataca gccagacaat cgaaaaagaa tcgattatct
    13681 tcgcacttac cttcacgaat atacgggcct agtacggtaa attttttgat caatctgctc
    13741 ttcgggtgtc cactcggtaa cgtcattgct atctcctgtt gtgatgcaac tgttttacca
    13801 aattggcaaa atttagcaaa tttttaacac aagcgttttt cgatccagtc ggtgatttcc
    13861 tgaagacctt tgtcaaaatt ccgatacacc gggttaaatg ggatctctaa taatttaccg
    13921 tcagcagatg atgaggtgat taagcgtgag tcctcttccg ggctgaacgg atcgttcttc
    13981 cagtagcctg ataacattgg cgttgggcat cgacgtccca gcaatccttg cacttttaat
    14041 gaatagcgat tcagctccac gcgcaacgct tcgtcggaag catcatgcat ccccaaacga
    14101 ctcgccagaa cgtcaagata catttccggc acctgttgct ggcacttaaa atcactcaac
    14161 agggtatgaa ctaccggacc aagacaggca accgctttca gacgcggcga ttcaagatat
    14221 gccagacgca cggcaacgtt agcgccgaaa cggaaaccaa aggccgcgac gcgagtgtga
    14281 tccacccacg gtacgttagg cagagccttt aagacgcgct gatgcaacag gcaggagtcc
    14341 tgggtgagct tccattttga agaaaagccc accgacggca tatcaatagt cagcatcgca
    14401 atgccgcgcg gcgcaaaata acgttcatac aggctgtaat agtccgtctg catcgcatcc
    14461 agaccaccac acattaatac cgtcgggaac gggccatcgc ctttcggcat atgcaaaaag
    14521 ccggtgatgg gcgcaccgcc gggtacggta aactccatct gtcgcatcgt gcccggtaga
    14581 cgctgagcgg cctcttcata ggcgcggttt gacaaagcct gcgcttgctc ggccaggtca
    14641 tctcctttca gatgaggata ggcggcaatg ttgtacaacg tagccgcatg cagccagtga
    14701 cgaccactga gttgcggatc ttcttccgca caggcttttt gttgccacac catcgcctgg
    14761 gtggcccact cataaatcca gttgccgcca cggtagccaa tcaccgtatc gtataaatca
    14821 tcgtcggtac gttcggcatc gctcatcaca atacgtgcct ggacgtcgag gatttcgcgt
    14881 gggtcaatgc cgcgccagat ccacatcaga cggttaatca tgcgatacca gtgagggatg
    14941 gttttaccat caagggccga ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact
    15001 agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc
    15061 tgtgtcatgg atgcagcctc cagaatactt actggaaact attgtaactc gcctgaagtt
    15121 aaaaagaaca acgcccggca gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg
    15181 gacgaatacg acgcccatat cccacggctg ttcgatccag gtatcttgcg ggatatcaac
    15241 aacataatca tcaaccagcg gacgaccagc cggttttgca aagatggtga caaagtgcgc
    15301 ttttggatac atttcacgaa tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat
    15361 aacgatgaag ccttcgccat cgccttctgc gcgtttcagc actttaagct cgcgctggtt
    15421 gtcgtgatcg tagctggaaa tacaaacggt atcgacatga cgaataccca gttcacgcgc
    15481 cagtaacgca cccggtacca gaccgccacg gcttacggca ataatgcctt tccattgttc
    15541 agaaggcatc agtcggcttg cgagtttacg tgcatggatc tgcaacatgt cccaggtgac
    15601 gatgtatttt tcgctcatgt gaagtgtccc agcctgttta tctacggctt aaaaagtgtt
    15661 cgaggggaaa ataggttgcg cgagattata gagatctggc gcactaaaaa ccagtatttc
    15721 acatgagtcc gcgtcttttt acgcactgcc tctccctgac gcgggataaa gtggtattct
    15781 caaacatatc tcgcaagcct gtcttgtgtt gacaacattt tctgctaacc ctgtgacctg
    15841 caatactgtt ttgcgggtga tcgacaagga gacttaacgt gtctgaactg tctcaattat
    15901 ctccacagcc gctgtgggat atttttgcca aaatctgttc tattcctcac ccgtcctatc
    15961 atgaagagca actcgctgaa tacattgttg gttgggcaaa agagaaaggt ttccatgtcg
    16021 aacgcgatca ggtaggtaat atcctgattc gtaaacctgc taccgcaggt atggaaaatc
    16081 gtaaaccggt cgtcttgcag gcccacctcg atatggtgcc gcagaaaaat aacgacaccg
    16141 tgcatgactt cacgaaagat cctatccagc catatattga tggcgaatgg gttaaagcgc
    16201 gcggcaccac gctgggtgcg gataacggca ttggtatggc ctctgcactg gcggttctgg
    16261 ctgacgaaaa cgtggttcac ggcccgctgg aagtgctgct gaccatgacc gaagaagccg
    16321 gtatggacgg tgcgttcggc ttacagagca actggttgca ggctgatatt ctgattaaca
    16381 ccgactccga agaagaaggt gaaatctaca tgggttgcgc ggggggtatc gacttcacct
    16441 ccaacctgca tttagatcgt gaagcggttc cagctggttt tgaaaccttc aagttaacct
    16501 taaaaggtct gaaaggcggt cactccggcg gggaaatcca cgttggcctg ggtaatgcca
    16561 acaaactgct ggtgcgcttc ctggcgggtc atgcagaaga actggacctg cgtcttattg
    16621 atttcaacgg cggcacactg cgtaacgcca tcccgcgtga agcctttgcg accattgctg
    16681 tcgcagctga taaagtcgac gccctgaaat ctctggtgaa tacctatcag gagatcctga
    16741 aaaacgagct ggcagagaaa gagaaaaatc tggccttgtt gctggactct gtagcgaacg
    16801 ataaagctgc cctgattgcg aaatctcgcg atacctttat tcgtctgctg aacgccaccc
    16861 cgaacggtgt gatccgcaac tccgacgtgg caaaaggtgt ggtcgaaacc tccctgaacg
    16921 tcggtgtggt gaccatgact gacaataacg tagaaattca ctgtctgatc cgttctctga
    16981 tcgacagcgg taaagactac gtggtgagca tgctggattc gctgggtaaa ctggctggcg
    17041 cgaaaaccga agcgaaaggc gcatatcctg gctggcagcc ggacgctaat tctccggtga
    17101 tgcatctggt acgtgaaacc tatcagcgcc tgttcaacaa gacgccgaac atccagatta
    17161 tccacgcggg cctggaatgt ggtctgttca aaaaaccgta tccggaaatg gacatggttt
    17221 ctatcgggcc aacaatcacc ggtccacact ctccggatga gcaagttcac atcgaaagcg
    17281 taggtcatta ctggacactg ctgactgaac tgctgaaaga aattccggcg aagtaattat
    17341 ttgatttgct gccggatggc gtttaatcgc cttccggcag tttcatcctt cattatcctt
    17401 cgataaaagc catccctgtg aacgttcgtc gcgggttgcc acgttcaatc tggtgatgga
    17461 acattcgccg ctgcgatttc agcactgcgc tattttcctg ttgctgctgc tccagcttcc
    17521 aggcaatcag caatcgcgcc agccgtttgt tagcatgctg gctgcgttct gactgaacct
    17581 tcacgctaat cccggttgcc agatgcgtgg cgcgtaccgc cgagtcggtt ttattgacat
    17641 gttgaccgcc cggccccgac ggacgcagcg tctcataacg gattgcatcc gattgttcct
    17701 gctcatcagc ggtaaaacgc ccaatgccca gaaaccagtt tttgcgccca tgatgaggcc
    17761 gatacggact cagacaaatc cactgaatag tgccgcacca actttcgctt aacgcccatg
    17821 cgttgtcgcc atccagagaa atcagcgccg aacgcagcgt gtcagagtag cggcccgttt
    17881 ctgtttccag caccgttacc gcgacgtctt gtcgggtaaa aacgaccaac agagaagaac
    17941 gctgcgccaa tcgggtagcc gcgtccgggg tgtaaatctg gcatccccac tacgcgctgc
    18001 atgttgggta aatttgccgt ggtgtgtaat tgttggatcg ctaaactctc gatccacagg
    18061 tcatcagatg cgatggtaaa taccgcatcg gataagggac gaatatattt gcccatgtac
    18121 cattccattt atactcgtca tacttcaagt tgcatgtact gcgactgcgt tcgttcaccc
    18181 cagtcactta cttatgtaag ctcctgggga ttcactcgct tgtcgccttc ctgcaactcg
    18241 aattatttag agtatataaa tataaaaatc aggaaatggc agacgaatcc agggcaagaa
    18301 aaaaaagaat taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat
    18361 ttacctcctt gcagtttgtg gaataagaaa acgcgcacat accaggcggg cgttattttc
    18421 attgcaagct ggatttaatg ttgcggttta tatcgcatag aaaaattagt aaaccacgct
    18481 ccccggcatt caacgcgctg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt
    18541 tttgcggtta tgctggcgtc caccgtaagt tcggattcag acttaatcca aggttttaac
    18601 aaagcgctgg caaccccacg gcgggtgtat tcagggtcaa caaataacat atcgatataa
    18661 tgttcaatgc gggaaataaa accaaccggt tgtgcattaa tgactgcaac ccgcacttgt
    18721 gatttcgcga gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt
    18781 tgtggtgaat aatgctgact ggcggtcatc ataaccgctc taatgaaaat agcgcatagt
    18841 tgctgaaaat cgccaggctg atagtttctt atttgtatgt tattcataat ataaattcaa
    18901 aaacgcatgc gaaacgcttc tgccattttc ccaattttat gcatttggtt gttatctcga
    18961 gccagtttat gaggttcagg tttcagaatg gcaatgagca accatgcttg ctcatcaaac
    19021 gcaccctgac aatataccaa atgcgattcg tcattcgttc tgctgaattg gcgcaactgt
    19081 ggcggaaatg gattattcac atttgccaga tgaatatgag caactcgctc aaatttgatt
    19141 aatggccaag taaacgagtc gtcgtagagt gcatcgcgac caaatatatc tggcaaaaca
    19201 ccgtcacgct tataggaaat aaaatccgcc gttaacgcat caagctcctc tgctgtaagt
    19261 tgcaggcgaa taagttttgt tttgaatacc cgcatcctta ttccttaaag tcattgaaat
    19321 catcatccgt catatcatta agatattttt cagctcggcg tgtaatttcc tctaatcttg
    19381 cagcacttgg acggaagcgc gcctttatgg gctttttctc ctcttgttca gcaagcatgt
    19441 ccattaacgc ttcgaatgcg ctggcactta agagatatcc tgcggggcga ttattagaaa
    19501 gaaccgcaac cggttgatca ataaagtatt tagctgggtt tttacgtaat tcagtgatat
    19561 tgaccgattt ttcagcgaga attcgatgca tacagtgatc ccctcataat aatgcacacc
    19621 agaatataca taatagtata catcataatc ccagcaccag ttgtctttcc atttgagggt
    19681 caagcaacgt cacatgcagc cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc
    19741 aggttttacg cgcggtggcg attaagtcag ctttattcag ccgcggccag acgtgctcct
    19801 gggtggtttg ctgaaaatcg tcgaacttta atttcacccc ctggcgagca atcagtaaat
    19861 caggttttac ctttgccaga cggcgttcaa gttccggata cagccgctcg ataatcgctt
    19921 cacattcaga ccagtgatga atatcttccg ccatcgtgcg ttccacgccg acggattttc
    19981 gtaaccgttc gctgttaacg tcgcgctcgt caatcccctg actacgctcc cacaaaatgc
    20041 ggccaaattt gccaaagcgt ttaagcagaa tcaccagatc acacttttgt acatcaccgc
    20101 aggtccgcag ccccatcgct tccagctttg ccgctgagac tttgccaaca ccgggaattt
    20161 ttgccagcgg taaggtttgc agaaatgccg aaacttctgc cggcgtaatc acaaactggc
    20221 cgttgggttt attcatgtcg gaggcgattt tggcgagaaa ctttactggt gccacgcccg
    20281 cagacgccgt cagttgcagc tcgctgaaaa ttgtctggcg gatttcctgg gcgatgaggg
    20341 tcgcagaacc gtggcaatgg acgctatcag tgacatcgag gtaggcttca tccagtgaca
    20401 acggttcaat acgcgaggtg tagcgcgaga agatttcacg gatatgattt gaggcttctt
    20461 tgtaggcgtc aaagcgcccc ggaagcaagg tgagatgtgg gcataatttg agcgccatcc
    20521 ctgtcggcat agcgctgcgt acgccaaatt tacgcgcggg ataattggcg gtgctgatca
    20581 ccccccgacg ttcgcggctg ccgccaatag caatagggat atcgcgcagg gcgggattgt
    20641 cgcgcatctc cactgcggcg aaaaagcagt ccatatccac atgaatgatt ttacgcatta
    20701 ctcacctctc aacactggta aagtatacag tgatttcagg gtttgagaaa tgcgtaaaga
    20761 ttcagcattt agcgcgttcg ctgcgaaagc acctgctgct tatccagctt ttgcaccagc
    20821 ggctgcacca ctttatcccc atgctgacca aagtattgat acagcgtatc ggacgcactt
    20881 ttggtcagca ccataatctc aatgcgtcgg ttgcccgcgc tttgcggatt tttcgcgtcc
    20941 agcagcatct ggtccgccat tgcgcttacc tgcatcactt tatcttccgg cattccggcc
    21001 tcttccagca cccgacgagc tgaaagcgcg cgatcgcccg aaaggttcca gttgttgtag
    21061 atattgtttt tgtaggccat cgcatcggta tgcccggtaa taataatttt gttatcgagc
    21121 gagtcgaaca ctggcgcgag ctccaccagc aaagttttaa agaacggcat aatctgcgcg
    21181 ctgccgcgtt cgaacatatt gcgattctgg tcgtctttaa tcagcacgcg taatccctgc
    21241 gggacaatct ccatctccag attcgcttcc atatgcgcat cacgggcaat ggtgttgata
    21301 ctggttgcca gctcgccaag ctcggtggcg gatttcttct tcagcagcgc cgttttctca
    21361 ttcacatcac gcgctttttt ctccgtctct tccggcaccg ccacggtggc aggttttggc
    21421 ggatgagacg ggctgatttt atttaacggc gacagcccac caccgttaaa aatcgactgc
    21481 ccgtgcagcg cggcgataat gctttcacgc tcggatttac tgacgctgtt gacaatcaac
    21541 tttatcgtcg tcaccaaagg ggccgagcgt atttccgagg tttctgcccg ctttaccctg
    21601 gatgcgatgc ccggcaaaca gatggcgatt gacgccgatc ttaacgccgg attgatcaac
    21661 caggcacagg cgcaaacccg gcgtaaagat gttgccagcg aggccgattt ctacggcgcg
    21721 atggacgggg catcgaagtt tgtgcgcggg gatgccatcg ccgggatgat gattctggcg
    21781 attaacctga tcggcggcgt ctgtatcggg atatttaaat acaacctgag cgccgacgcc
    21841 gccttccagc aatatgtgct gatgaccatc ggcgatgggc tggtggcgca gatcccttcc
    21901 ctcctgctct ccaccgcagc ggcgattatc gtcacccgcg tcagcgacaa cggcgatatc
    21961 gcccatgacg tgcgccacca gctgctggca agcccgtcgg tgctctacac cgccaccggg
    22021 attatgtttg tgctggcggt ggtgccggga atgccgcacc tgccgttttt gctgttcagc
    22081 gccctgctgg gttttaccgg ctggcggatg agcaaacagc cgcaggcggc ggaggcggaa
    22141 gagaaaagcc tcgaaacgct gacccgcacc atcactgaaa ccagcgagca acaggtcagt
    22201 tgggaaacca ttccgcttat cgagcctatc agtttaagcc tcggctacaa gctggtggcg
    22261 ctggtcgata aagcgcaggg caacccgctc acccagcgta ttcgcggcgt acggcaggtg
    22321 atatccgacg gtaacggcgt gctgctgccg gagatccgca ttcgggaaaa cttccgcctc
    22381 aagcccagcc agtacgccat tttcatcaac ggcattaagg ccgatgaagc ggatattccg
    22441 gcggataaac tgatggcgct gccctccagc gaaacctacg gcgagattga cggcgtgctg
    22501 gggaacgacc cggcgtacgg gatgccggtg acctggatcc agcccgcgca gaaggcgaag
    22561 gcgctgaata tggggtatca ggtgatcgac agcgccagcg tgattgccac gcatgtgaac
    22621 aagattgtgc gcagctatat tcctgatttg tttaattatg atgacatcac gcagctgcat
    22681 aaccgcctgt cgtcgatggc cccgcgcctg gcggaagatt tgagcgcggc gctgaattac
    22741 agccagttgc tgaaagtgta ccgtgcgctg ctgaccgaag gcgtttccct gcgcgatatc
    22801 gtcaccatcg ccaccgtgct ggtcgccagt agcgcggtga ctaaagatca tattctgctg
    22861 gcggccgatg tgcgcctggc gctgcggcgc agcattaccc atccgttcgt tcgcaagcag
    22921 gagctgacgg tgtatacgct gaataatgag ctggaaaatc tgctgactaa cgtagtgaat
    22981 caggcgcaac agggcggtaa agtgatgctc gacagcgtgc cagtggaccc gaatatgctc
    23041 aaccagttcc agagcacgat gccgcaggtg aaagagcaga tgaaagcggc ggggaaagac
    23101 ccggtgctgc tggtgccgcc gcagctgcgc cctttgctgg cgcgctatgc aaggttgttt
    23161 gcgccggggc tgcatgtgct gtcgtataac gaagtgccgg atgagctgga gttgaagata
    23221 atgggggcgt tgagttgatt ggcctttaag gggcgcatcc ggcattggtg cacaattgcc
    23281 ggatgcggcg tgaacgcctt atccggccta cggttgagtg cagttttgta ggcatgataa
    23341 gacgcgccag cgtcgcatca ggcatccggc accagcgcac aattatgaaa tgatacgctc
    23401 cccggcacta aaatccgtta tgtctcccgc ccaatcaatg ggatataacc ctcgcgccac
    23461 atcgcgatgg aacgttgaga atggccaatc actcacttgc tttacccagc catgttttac
    23521 tggatttata taaatataat caacatgatg gcgatagtct ttggtattgc ggatggtgtg
    23581 ctcctaaaaa cgtggttgcc agatattttt caatccacaa gcatgggtga actgcttttt
    23641 aatctcccgc cagcgggagg aaaaatcatc atcgctttcg ggtaatgtcc atatgcaatg
    23701 catatgctcc ggcaaaacga cccaggcgtt aatttcaaaa ggtctgtctc gcttaacgtt
    23761 aataatggcg ttacggagca tctggaactg agcggtcaga aggtggcttc gacgatttcg
    23821 taggttaacc gtgaaaaacc aggttccccc tttgatgtaa taccgacgat attcagacat
    23881 ggcaactcct ttttctcaac ggtaagaaga aaaggaaagg tcggcaatga ggtgatggaa
    23941 taacgggaaa gcggctcgca aaaacgcaca gatttgtagg catgataaga cgcgtcagcg
    24001 tcgtatcagg catctgcgca caactgccgg atgcggcgtg aatgccttat ccgtcctaca
    24061 aaatcgtctc ttccgtcaaa atccggcgcg cgcctaaaaa gtgatcctgc cagaaaggtt
    24121 cagctaaccg acttacccga atggtttccc cggtgcgcgg tgactcaata aactgaccgt
    24181 cgcccagata cacccccata tggtcggcaa tctcgcggct gtggatatgg aaaaacagca
    24241 aatctccccg gcgcaggtcg ttattcgcca caatcgttgc ccgatggtag tgatacatct
    24301 cattagccgt acgcgggagc ttcgcctcga ggatcttgtt gtaggcataa aaaaccaacc
    24361 cgctacagtc aaagccttta tcaggccgcg tgccgcccca gacgtaaggc ttgccaagct
    24421 gctgctccag gcggtgaatc gccacttcgg taatgttatg cagatggtcg ctgcttaaaa
    24481 agtggttgtt ttccgccagc gcctgccagc gtctgtcact cgccttgagc ggtgcaggaa
    24541 accattctgg atgctgtttt atctgttcgc ggttgtgctg gcgtagcgcc cttctgcttt
    24601 cggcattgcc ttccacaata tagctgacct gctttttcag atgggtttgg tactgtttta
    24661 ataaacgcgc acggttttgc atccgctggc gggcggcgta gctgaaagag atatgtgatg
    24721 tggtggcgct ggcaaatgaa gaaaacggca aacaaatcaa cagtaatccc gacaatacaa
    24781 aggatggaat cgacggcaag gacatactca ataatggctg gtcatgcatt gatgttacag
    24841 tgtatcccgg cccgtacctg aacaaactta ataaaccatt attataaaat taa
//
LOCUS       DAZGXS010000067        24120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGXS010000067 DAZGXS010000000
VERSION     DAZGXS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            188..523
                     /gene="yjdM"
                     /locus_tag="ACHKG5_002766"
     CDS             188..523
                     /gene="yjdM"
                     /locus_tag="ACHKG5_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0868838.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            782..1225
                     /gene="yjdN"
                     /locus_tag="ACHKG5_002767"
     CDS             782..1225
                     /gene="yjdN"
                     /locus_tag="ACHKG5_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0868839.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1358..2179
                     /gene="phnC"
                     /locus_tag="ACHKG5_002768"
     CDS             1358..2179
                     /gene="phnC"
                     /locus_tag="ACHKG5_002768"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0868840.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQRVAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVV
                     VTLHQVDYALRYCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2204..3220
                     /gene="phnD"
                     /locus_tag="ACHKG5_002769"
     CDS             2204..3220
                     /gene="phnD"
                     /locus_tag="ACHKG5_002769"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0868841.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3326..4105
                     /gene="phnE"
                     /locus_tag="ACHKG5_002770"
     CDS             3326..4105
                     /gene="phnE"
                     /locus_tag="ACHKG5_002770"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0868842.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4126..4851
                     /gene="phnF"
                     /locus_tag="ACHKG5_002771"
     CDS             4126..4851
                     /gene="phnF"
                     /locus_tag="ACHKG5_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0868843.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4852..5304
                     /gene="phnG"
                     /locus_tag="ACHKG5_002772"
     CDS             4852..5304
                     /gene="phnG"
                     /locus_tag="ACHKG5_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0868844.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5301..5885
                     /gene="phnH"
                     /locus_tag="ACHKG5_002773"
     CDS             5301..5885
                     /gene="phnH"
                     /locus_tag="ACHKG5_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0868845.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5885..6949
                     /gene="phnI"
                     /locus_tag="ACHKG5_002774"
     CDS             5885..6949
                     /gene="phnI"
                     /locus_tag="ACHKG5_002774"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0868846.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6942..7787
                     /gene="phnJ"
                     /locus_tag="ACHKG5_002775"
     CDS             6942..7787
                     /gene="phnJ"
                     /locus_tag="ACHKG5_002775"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0868847.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7784..8542
                     /gene="phnK"
                     /locus_tag="ACHKG5_002776"
     CDS             7784..8542
                     /gene="phnK"
                     /locus_tag="ACHKG5_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0868848.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8653..9333
                     /gene="phnL"
                     /locus_tag="ACHKG5_002777"
     CDS             8653..9333
                     /gene="phnL"
                     /locus_tag="ACHKG5_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0868849.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9330..10466
                     /gene="phnM"
                     /locus_tag="ACHKG5_002778"
     CDS             9330..10466
                     /gene="phnM"
                     /locus_tag="ACHKG5_002778"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0868850.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10466..11023
                     /gene="phnN"
                     /locus_tag="ACHKG5_002779"
     CDS             10466..11023
                     /gene="phnN"
                     /locus_tag="ACHKG5_002779"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0868851.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11010..11445
                     /gene="phnO"
                     /locus_tag="ACHKG5_002780"
                     /pseudo
     CDS             11010..11445
                     /gene="phnO"
                     /locus_tag="ACHKG5_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11447..12205
                     /gene="phnP"
                     /locus_tag="ACHKG5_002781"
     CDS             11447..12205
                     /gene="phnP"
                     /locus_tag="ACHKG5_002781"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0868852.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12282..12611)
                     /gene="yjdP"
                     /locus_tag="ACHKG5_002782"
     CDS             complement(12282..12611)
                     /gene="yjdP"
                     /locus_tag="ACHKG5_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0868853.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12680..14950)
                     /locus_tag="ACHKG5_002783"
     CDS             complement(12680..14950)
                     /locus_tag="ACHKG5_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0868854.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15064..16584
                     /locus_tag="ACHKG5_002784"
     CDS             15064..16584
                     /locus_tag="ACHKG5_002784"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0868855.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16581..17555
                     /locus_tag="ACHKG5_002785"
     CDS             16581..17555
                     /locus_tag="ACHKG5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0868856.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17574..18515
                     /locus_tag="ACHKG5_002786"
     CDS             17574..18515
                     /locus_tag="ACHKG5_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0868857.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18570..19253
                     /locus_tag="ACHKG5_002787"
     CDS             18570..19253
                     /locus_tag="ACHKG5_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP0868858.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19268..20128
                     /locus_tag="ACHKG5_002788"
     CDS             19268..20128
                     /locus_tag="ACHKG5_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFP0868859.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQT"
     gene            20125..21126
                     /locus_tag="ACHKG5_002789"
     CDS             20125..21126
                     /locus_tag="ACHKG5_002789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0868860.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21123..21830)
                     /locus_tag="ACHKG5_002790"
     CDS             complement(21123..21830)
                     /locus_tag="ACHKG5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP0868861.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22121..24106
                     /gene="yjcS"
                     /locus_tag="ACHKG5_002791"
     CDS             22121..24106
                     /gene="yjcS"
                     /locus_tag="ACHKG5_002791"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP0868862.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5367 a         7024 c         6757 g         4972 t
ORIGIN      
        1 atcttaaata ataaatggag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
       61 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      121 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      181 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      241 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
      301 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      361 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      421 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      481 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      541 agccggataa ggcgtttacg ccgcatccgg caacagtgcc agatgcctga tgcgacgctc
      601 gcgcgtctta tcaggcctac aaattcccgc accctccgta ggccggataa ggcgtttacg
      661 ccgcatccgg caacagtgcc gactgcctga tgcgacgctc gcgcgtctta tcaggcttcc
      721 ctctcatctg tataaatttc gaactacact taactggctt ctcttaactg aggtcaccat
      781 catgccgtta agtccctacc tctcttttgc cggtaactgc tccgacgcga ttgcctatta
      841 tcaacgtacg ttgggcgctg aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc
      901 agcgcaggac agcgccgaga actgcccttc cggaatgcaa tttcccgata ccgccatcgc
      961 ccacgccaat gtgcgcattg ccggaagcga tatcatgatg agcgatgcta taccgtcagg
     1021 aaaagccagc tactccggtt ttacgctggt gctcgattcg caacaggtcg aagaaggaaa
     1081 acgctggttt gataaccttg ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt
     1141 ctgggcgcat ggctttggca aagtcaccga taaatttggc gtaccgtgga tgattaatgt
     1201 cgtcaaacaa caaccaacgc aataatccgc cgggaggccc gccctcccgc actgtcatcg
     1261 aattcccgtt aactcttcat ctgttagtca cttttaatta accaaatcgt cacaataatc
     1321 cgccacgatg gagccacttt tttagggagg ctgcatcatg caaacgatta tctgcgtcga
     1381 acaactgagc aaaaccttca accagcatca ggcgctgcac gcggttgatc tgaatatcca
     1441 tcatggtgaa atggtggctc tgcttgggcc gtccggttcc ggcaaatcca cccttttacg
     1501 tcacttaagc ggtttgatta ccggcgataa atccgccggt agccatatcg agctgctggg
     1561 ccgcactgtc cagcgcgaag gccgcctggc ccgcgatatc cgcaaaagcc gcgccaacac
     1621 cggctacatc ttccaacaat tcaacctggt gaatcgcctg agcgtactgg agaacgtgct
     1681 gattggcgcg ctcggcagca cgccgttctg gcgcacctgt tttagttggt tcacccgcga
     1741 gcagaaacag cgcgcattac aggcgctgac ccgcgtcggt atggcgcact ttgcgtatca
     1801 gcgtgtttcc acgctctccg gcggccagca gcagcgtgtg gcgattgccc gcgcgctgat
     1861 gcagcagcgt gtggcgattg cccgcgcgct gatgcagcag gcgaaagtga ttctcgctga
     1921 cgaaccgatc gcctcgctgg acccggaatc ggcgcgcatc gtgatggaca ccctgcgcga
     1981 catcaaccag aacgacggca tcaccgtggt cgtcacgctg catcaggtgg attacgccct
     2041 gcgctactgc gaacgcatcg tcgccctgcg ccaggggcac gtcttctacg acggcagcag
     2101 ccaacagttt gataacgaac gttttgacca tctctaccgc agcatcaacc gcgtcgaaga
     2161 gaacgcgaaa gctgcctgac atccccatca ttgaggaaaa cgaatgaacg ctaagataat
     2221 tgcctcgctg gccttcacca gcatgttcag cctcagcacc ctgttaagcc cggcacacgc
     2281 cgaagagcag gaaaaggcgc tgaatttcgg cattatttca acggaatcac agcaaaacct
     2341 gaaaccgcaa tggacgccgt ttttacagga tatggagaag aagctgggcg tgaaggtcaa
     2401 cgccttcttt gccccggact atgcgggcat tatccaggga atgcgtttca acaaagtgga
     2461 tatcgcctgg tacggcaacc tgtcggcgat ggaagcagtg gatcgcgcca acggccaagt
     2521 cttcgctcag accgtcgcgg cggatggatc gccgggttac tggagcgtgt tgatcgtcaa
     2581 caaagacagc ccgatcaaca acctgaacga tctgctggcg aagcggaaag atctcacctt
     2641 cggtaatggc gatcctaact ccacctctgg cttcctcgtc cccggctact acgtcttctc
     2701 caaaaacaat atctccgcca gtgacttcaa gcgcaccgtc aacgccgggc atgaaaccaa
     2761 cgcgctggcc gtcgccaaca agcaggtgga tgtggcgacc aacaacaccg aaaacctcga
     2821 caagctgaaa acctccgcgc cggagaagct gaaagaactg aaagtgatct ggaagtcacc
     2881 gctgatccca ggcgatccga tcgtctggcg taaaaacctt tccgaaacca ccaaagacaa
     2941 gatctacgac ttctttatga attacggcaa gacgccggaa gagaaagcgg tgctggaacg
     3001 cctgggctgg gcgccgttcc gcgcctccag cgacctgcaa ctggtgccga ttcgccagct
     3061 cgcactgttt aaagagatgc aaagcgtgaa aggcaataaa gggctgaatg agcaggacaa
     3121 gctggcaaaa accaccgaga ttcaagcgca gctggatgac ctggaccgcc tgaacaacgc
     3181 gttaagcgcg atgagttcgg taagtaaagc gatgcagtaa atcgtaggtc ggataagacg
     3241 ccccggtgtc gcatccgaca atgtgcaggc gttgatgccg gatgcggtgc aagcacctta
     3301 tccggcctac agaccggagc caaacatgca aaccatcacc atcgccccac ccaagcgcag
     3361 ctggttctcg cttctgagct gggccgttgt actcgccgtg ttggtcgtct cgtggcaggg
     3421 cgcggaaatg gccccgctca cgctgatcaa agacggcggc aacatggcga ccttcgccgc
     3481 cgacttcttc ccgcccgatt tcagccagtg gcaggattac ctcaccgaaa tgctcgtcac
     3541 gctgcaaatc gccgtctggg gcaccgcgct ggcggtggtt ctctccatcc ccttcggcct
     3601 gatgagcgcc gaaaacctgg tgccgtggtg ggtttaccag cccgtgcgcc gcctgatgga
     3661 cgcctgccgc gccattaacg aaatggtctt cgccatgctg ttcgtggtcg ccgtcggcct
     3721 cggcccattc gctggtgtgc tggcgctgtt tatccacacc accggtgtgc tctccaaact
     3781 gctttccgaa gcggtggaag cgattgaacc cggcccggtg gaaggcattc gcgccaccgg
     3841 cgccaacaag ctcgaagaga tcctctacgg cgtgctgccg caggtgatgc cgctgctgat
     3901 ctcctactcc ctctatcgct tcgaatccaa cgtccgctcg gcgaccgtcg tcggcatggt
     3961 cggcgcgggc gggatcggcg tcacgctgtg ggaagcgatt cgcggtttcc agttccagca
     4021 aacctgcgcc ctgatggtgc tgattatcgt cacggtcagc ctgctggatt tcctctctca
     4081 acggttgcgt aagcacttta tctgattagc gaggcattga tagctatgca cttgtctaca
     4141 catccgacca gctacccaac acgctatcag gagatagccg caaaacttga gcaggagctt
     4201 cgtcaacact accgctgcgg cgactatctt cccgccgagc agcagctggc ggcgcgcttt
     4261 gaggtaaatc gccacaccct gcgccgcgcc atcgaccaac tggtggaaaa aggctgggta
     4321 cagcgccgtc agggcgtcgg cgtgctggtg ctgatgcgcc cgttcgatta cccgctcaac
     4381 gcccaggcgc gttttagcca gaatctgctg gatcagggca gccatcccac cagcgaaaaa
     4441 ctgctgtctg tgctgcgccc ggcctccggt cacgtcgctg acgcgctggg gataaccgag
     4501 ggggagaacg tcatccatct gcgcaccctg cgccgggtca acggcatcgc gctctgttta
     4561 atcgaccact acttcgcgga cctcaccctc tggccgacgc tgcaacgctt cgacagcggc
     4621 tcgctgcacg attttctgcg cgagcaaacg gggattgcac tgcgccgcag ccagacgcgg
     4681 atcagcgccc gccgcgccca ggccaaagag tgccagcgtc ttgaaattcc gaatatgtcc
     4741 ccgctgctgt gcgtgcgcac ccttaaccac cgtgacggtg aaagcagccc ggcggagtac
     4801 tccgtcagcc tgacgcgcgc cgacatgatt gaattcacta tggagcactg aatgcacgca
     4861 gataccgcga cccgccagca ctggatgtcc gtactggcac acagccaacc ggcagaactg
     4921 gcagcccgcc tgaacgcgct gaacatcacc gccaactatg aggtgatccg cgccgctgaa
     4981 actggcctgg tacagattca ggcgcggatg ggcggcaccg gcgaacgctt ttttgccggc
     5041 gacgccacgc tgacccgcgc cgccgtacgt ctgactgacg gcaccctcgg ctacagctgg
     5101 gtgctggggc gcgacaaaca gcacgccgaa cgctgcgcgc tgattgacgc gctgatgcag
     5161 caaacccgcc attttcaaaa cttatcagaa acgcttattg ccccgctgga cgctgaccgt
     5221 atggcacgca ttgccgcacg ccaggccgaa gtgaacgcca gccgggtcga cttctttacg
     5281 atggttcgcg gagacaacgc atgaccctgg aaaccgcttt tatgcttccc gtgcaggatg
     5341 cccagcacag ttttcgtcgc ctgttaaagg ccatgagcga gccgggcgtg attgtcgccc
     5401 tgcatcagct caaacgcggc tggcagccgc tgaatatcgc caccaccagc gtgctgctga
     5461 cgctggccga taacgacacg ccggtgtggc ttgccgcgcc attgagcaac gatatcgtca
     5521 gccagagcct gcgttttcat accaacgcgc cgctggtcag ccagccggaa caggcgacct
     5581 tcgcggtaac ggatgaggcg atttccagcg aacagcttaa cgccctttcc accggcaccg
     5641 ccgttgcgcc ggaagcgggc gcaacgctga ttttacaggt cgccagcctg agcggcgggc
     5701 gcatgctgcg tctcaccggc gcgggtattg ccgaagaacg aatgatcgcc ccgcagctgc
     5761 cggagtgcat tctgcacgaa ctcaccgagc gcccgcaccc gttcccgctc ggcatcgacc
     5821 tgatcctgac ctgcggcgag cgcctgctgg ctgttccgcg aaccactcat gtggaggtgt
     5881 gctgatgtac gttgccgtga aagggggcga gaaggcgatc gacgccgccc acgccctgca
     5941 agagagccga cgccggggcg ataccgattt gcctgaactg agcgtcgccc agattgaaca
     6001 gcagcttaat cttgcggtgg atcgcgtgat gaccgaaggc ggcattgccg accgcgagct
     6061 ggcggcgctg gcgctgaaac aggccagcgg cgataacgtc gaagcaattt tcctgctgcg
     6121 cgcctaccgc accacgctgg cgaagctcgc ggtaagcgag ccgctcgaca ccacggagat
     6181 gcgtctcgaa cggcgtattt cagcggttta taaagatatt cccggcggcc agctgcttgg
     6241 cccaacctac gactacaccc atcgcctgct cgatttcacc ctgctggcaa acggcgaagc
     6301 gccgacgctg accaccgccg acagcgaaca gcagccgtcg ccgcacgttt tcagcctgct
     6361 ggcgcgtcag gggctggcga agtttgaaga ggatagcggc gcacagccgg atgacatcac
     6421 ccgcacgcca ccggtttacc cctgctcacg ttcttcccgc ctgcaacagc tcatgcgcgg
     6481 cgacgaaggc tatttgctgg cgctggccta ctccacccaa cgcggttacg ggcgcaatca
     6541 cccgttcgcg ggtgaaatcc gcagtggtta catcgacgtg tcgattgtgc ccgaagagct
     6601 gggatttgcg gtgaacgtcg gcgaactgct gatgactgag tgtgaaatgg tcaacggttt
     6661 tatcgaccca ccggatgaat ctccgcactt cacgcgcggc tacgggctgg tgttcggcat
     6721 gagcgagcgc aaagcgatgg cgatggcgct ggtcgaccgc gctctgcaag ccccggagta
     6781 cggcgagcac gcgacaggcc cggcgcagga tgaagagttc gtgctggcac atgccgacaa
     6841 cgtcgaagcc gcaggctttg tctcgcacct caaactcccg cactacgtcg atttccaggc
     6901 cgaactggag ctactcaaac gtctgcaaca ggagcagaac catggctaat ctgagcggct
     6961 acaactttgc ctacctcgac gagcagacca aacgcatgat ccgccgcgcc atcttaaaag
     7021 cggtggcgat ccccggttat caggtgccgt ttggcgggcg cgagatgcca atgccgtacg
     7081 gctggggaac cggcggcatt cagcttaccg ccagcgtgat tggcgaaagc gacgtgctga
     7141 aggtgattga ccagggcgcg gatgacacca ccaacgccgt gtcgattcgc aacttcttta
     7201 agcgcgtgac cggggtaaac accaccgagc gcacggacga tgcaacggtc atccagacgc
     7261 gtcaccgcat ccccgaaacg ccgctgaccg aagatcagat cattattttc caggtgccga
     7321 tccccgaacc gctgcgcttt atcgagccgc gcgaaacgga aacccgcacc atgcacgcgc
     7381 tggaggaata cggcgtgatg caggtgaagc tgtatgaaga tatcgcccgc ttcggtcata
     7441 tcgccaccac ctacgcctat ccggtgaagg tgaacgggcg ctacgtaatg gacccgtcgc
     7501 cgatcccgaa attcgataac ccgaaaatgg acatgatgcc cgccctgcaa ctcttcggcg
     7561 ccgggcgtga gaagcgcatc tatgcggtgc cgccgtttac ccgcgtggaa agcctcgatt
     7621 tcgacgatca cccgttcacc gttcagcagt gggatgagcc atgcgccatc tgcggatcga
     7681 cccacagcta tcttgatgaa gtggtgctgg atgacgccgg aaaccgcatg tttgtctgct
     7741 ccgataccga ttattgccgc caacagagcg aggcaaaaag ccaatgaatc aaccgttact
     7801 ttcggtcaat aacctgaccc acctttacgc gccgggcaaa ggctttagcg atgtctcttt
     7861 tgatttatgg ccgggggaag tgctgggcat tgtcggggaa tccggctccg ggaagaccac
     7921 gctgctgaaa tcaatttccg cgcgtctgac gccgcagcag ggggagattc actatgaaaa
     7981 ccgttccctc tatggcatga gcgaggccga ccgccgtcgc ctgctgcgca ccgaatgggg
     8041 cgtagtgcat cagcatccgc tcgacggcct gcgccgccag gtctcggcag gcggcaatat
     8101 cggcgagcgg ctgatggcga ccggggcgcg tcattacggc gatattcgcg ccaccgcgca
     8161 gaagtggctg gaagaggtgg aaattcccgc caaccggatc gacgacctgc cgaccacctt
     8221 ttccggcggt atgcagcagc gtttgcagat tgcccgcaac ctggtgacgc atccgaagct
     8281 ggtgtttatg gatgaaccga ccggcgggct ggatgtgtcg gtgcaggccc gcctgctcga
     8341 cctgctgcgc ggcctggtgg tggagctgaa cctcgcggta gtgattgtca cccacgattt
     8401 aggcgtcgcc cgcctgctgg cggaccgttt gctggtgatg aagcaggggc aagtggtgga
     8461 gagtgggtta accgaccgcg tgctcgacga cccgcatcat ccgtatacac agctgctggt
     8521 gtcatcggtt ttgcagaatt gagccggtgc cggatgcggc gtaaacgcct tatccggcct
     8581 acaaatgcgc tccccgtagg tcggataaga cgcatcagcg tcgcatccga cacccgaacc
     8641 acgaggcgaa aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct gcaccagcaa
     8701 aacggcgtgc gcttgcccgt cctcaaccgc gcctcgctca ccgtcaacgc gggcgaatgc
     8761 gtggtgctcc acggccactc cggcagcggc aaatcaaccc tgctgcgctc gctgtacgcc
     8821 aactatctgc ccgatgaagg gcaaatccag atcaaacacg gtgacgagtg ggtagacctg
     8881 gtcactgcgc cagcgcgcaa agtggtggaa atccgcaaaa ccaccgttgg ctgggtgagc
     8941 cagtttctgc gcgttatccc gcgcatttcg gcgctggaag tagtaatgca gccgctgctc
     9001 gataccggcg taccgcgtga agcctgcgcc gctaaagccg cgcgtctgct cacccgtctg
     9061 aacgtgccgg aacgcctgtg gcgccttgcg ccatcgacct tctccggcgg cgaacagcag
     9121 cgcgtcaaca tcgcccgtgg ctttatcgtc gactacccca ttctgctgct cgacgaacct
     9181 accgcctcgc tggacgccaa aaacagcgcc gccgtagtcg aactgatccg agaagccaaa
     9241 gcccgcggcg cagccatcgt cggcattttc catgacgaag ccgtacgtaa tgacgtcgcc
     9301 gaccgcctgc acccaatggg agcctcttca tgattatcaa taacgtcaaa ctggtgctgg
     9361 aaaacgaagt ggtaaacggt tcgctggaga tgcaggacgg cgaaatccgc gcctttgccg
     9421 aaagccagag ccgcctgccg gaggcgatgg acggcgaagg cggctggctg ctgccggggc
     9481 tgattgagct gcatacagac aacctcgata aattcttcac cccgcgcccg aaagttgact
     9541 ggcccgccca ctcggcgatg agcagccatg acgcgctgat ggtggcgagc ggcatcacca
     9601 ccgtactgga cgccgtggcg attggcgacg tgcgagacgg cggcgatcgg ctggagaatc
     9661 tggagaagat gatcaacgcc atcgaagaga cgcagaaacg cggcgtcaac cgcgccgagc
     9721 accgcctgca cctgcgctgc gagctgccgc ataacaccac gctgccgctg tttgaaaagc
     9781 tggtgcagcg cgagccggtg acgctggtgt cgctgatgga tcactcgccg ggccagcgcc
     9841 agttcgccaa ccgcgagaag tatcgcgaat attatcaggg caaatactcg ctcaccgatg
     9901 cgcagatgca gcagtatgaa gaggaacagc tggcgcttgc cgcacgctgg tcgcagccga
     9961 atcgcgaatc catcgccgca atgtgccgcg cgcgacaaat tgcgctcgcc agccacgatg
    10021 acgcaacgca cgcccacgtt gccgaatctc accagcttgg cagcgtgatc gccgaatttc
    10081 ccaccacgtt tgaagcagcg gaagcctcgc gcaagcatgg catgaacgtg ctgatgggcg
    10141 cgccaaatat cgtgcgtggc ggttcgcact ccggcaacgt ggcggccagt gaactggcac
    10201 agcttggcct gctggatatc ctctcttccg actactaccc cgccagcctg ctggatgcgg
    10261 cgttccgcgt cgccgatgac cagagcaacc gctttacgct gccgcaggcg gtgaagctgg
    10321 tgaccaaaaa tccggcgcag gcgcttaatc tccaggatcg cggggtgatt ggcgagggca
    10381 aacgcgccga cctggtgctg gcgcatcgca agggcaatca cattcatatc gaccacgtct
    10441 ggcgtcaggg taaaagggtg ttctgatgat gggaaaactg atttggttaa tggggccgtc
    10501 cggctccggg aaagatagcc tgctggcgga actccgcctg cgggaacaaa ctcagctact
    10561 ggtggcgcat cgttacatca cgcgcgacgc cagcgccgga agcgaaaacc atatcgccct
    10621 gagcgagcag gaatttttta cccgcgcggg gcaaaatctg ctggccttaa gctggcacgc
    10681 taacggtctg tattatggcg tcggcgtcga gattgacctc tggctgcacg ctggattcga
    10741 cgtgctggtc aacggctcac gcgcccatct gccgcaggcg cgggcgcgct atcaatcggc
    10801 gctgctgccc atttgcttac aggtttcgcc agagatcctg cgccagcgcc tggaaaaccg
    10861 tggccgcgaa aacgccagtg aaattaacgc ccggctggcg cgcgccgccc gctatactcc
    10921 acaggattgc catacgctca ataatgacgg cagcctgcgc cagtcggtcg acacgctgct
    10981 gacgctaatc catcagaagg agaaacacca tgcctgcttg tgagctttcg cccggccacg
    11041 cagtacgaca ccgacgcggt ttacgcgttg atttgcgagc taaaacaggc ggagtttgac
    11101 caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgaacat gcgctaccat
    11161 ctggcgctgc tggatggcga agttgtcggt atgatcggcc tgcatttgca gtttcatctg
    11221 catcatgtca actggatcgg cgaaattcag gagttggtgg taatgccgca ggcgcgcggt
    11281 ctgaacgtcg gcagtaagtt actggcgtgg gcagaagaag aagcccgcca ggccggggcc
    11341 gaaatgaccg aactttcgac caacgtgaag cgccacgacg cgcaccgttt ctatctgcgc
    11401 gaaggctacg agcagagcca cttccgcttc accaaggtgc tgtaacatga gcctgaccct
    11461 cacgctcacc ggctccggcg gcgcacaggg cgttccggca tggggctgcg agtgtgcggc
    11521 ctgcgccaga gcgcggcgct cgccgcacta tcgccgccag ccatgcagcg gcgtagtgaa
    11581 gtttaacgag gcgatcaccc tgatcgacgc cgggcggcac gatctcgccg atcgctggtc
    11641 gcccggatcg ttccagcagt ttttgctgac gcactaccat atggatcacg tgcaggggct
    11701 attcccgctg cgctggggcg tgggcgatcc gatcccggtt tacggcccac cggatgaaca
    11761 gggctgcgac gatctgttta aacatccggg cctgcttgat ttcagccaca cggtggaacc
    11821 gtttgtggtg tttgatttgc aggggttaca ggtcacaccc ctgccgctca accactcaaa
    11881 actgaccttc ggttatctgc tggaaacggc gcacagccgg gtggcgtggc tgtctgacac
    11941 cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat aatcagccgc aggtaatggt
    12001 gattgattgc agccacccgc cgcgcgagga tgcaccgcgt aatcactgtg atttaaatac
    12061 cgtgcttgcg ctgaatcagg ttatccgctc gccgcgcgtg gttctgaccc atatcagcca
    12121 ccagtttgat gcgtggctga tggaaaacgc actgccgtcg gggtttgagg cggggtttga
    12181 tgggatggag attggggtgg cgtgatgaga gggaatgtgc gtgctggccc cctcacccta
    12241 accctctccc cagaggggcg atgcctgccc catacccaac atcattgccc atactcatct
    12301 tccattctcc gctcttcatc ttctagctgc cgacgctcct gattaagctg gtgctggcga
    12361 tcgtccagct gcctgcgacg atcttcaaac tggcgacggc ggtcgtcata ttgtctgcgc
    12421 cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg aatcttcata atctcgccca
    12481 cggtcagggt tagaagcgtc gttaatcgcc tgctgaatat tgccaatggt gtcatcgata
    12541 atatcggcct gggccggtac ggtggtcagc gtgagcaggg tgaaaagaaa aagcgtggag
    12601 tggcgtttca taaactcggc ctcgcaacaa agttaataca aggtaggcaa aggtgcctgt
    12661 tacgagtaga ggaggattct caattctctt gccccagcgc ctgcgccaac tgcgcacggg
    12721 taaagggttt gcgcaacagc gccacgtctg gcagcgcggg gttatggctg gggcgtaaat
    12781 cctgaccgct gatgagtagc agcgtcaggt gcgggtaaag tttgcgggcc gcattgacca
    12841 cttctgcgcc gctcatgccg ccggggagca ttaaatcgct gataaaaata tcgatttctg
    12901 ctgatgccgc cagcagatcc agcgcctgtt cgccgctgct tgcttccagc gtcaaatagc
    12961 ccagcaggtg caactgttcg cagatggtct ggcgcaccgc ggcttcatct tccagcacta
    13021 acaccagttt atcgccgctg acagaagcct gttctactgc cgctggcgat aggtttttcg
    13081 ccgccgtcag cgcgcgggga agctgtaatc tgaccgttgt cccctgcccc ggcgcgcttt
    13141 caatttcaac gcgcccaccg gactggcgca caaaaccata gaccatcgac aaacccagcc
    13201 cgctgccgct gcccgtctgt ttggtggtaa agaatggctc aaatacctgg cttttcaccg
    13261 cctgcgacat accgctgcca tgatcgatga cctccagtgc caccatgtcc tgacgctgcc
    13321 cactgctgcg agtaacgcgc tgattccagg tacgaatttt gatgattccc gtctgccctt
    13381 ccatcgcgtc acgggcgttc atcaccaggt tgatgatggc attttccagt tggctgatgt
    13441 caatccacgc gggccacgcc ggagactgcg cttcaatctc cagcgccagc gttgccggta
    13501 aggagtggcg cattaattcg cttaactctt ccagcaatgt tttcagttca acggcttgcg
    13561 gatgtaagga ctgcttgcgg gaaaacgcca gcagtcgttg tgtcagtagc gccccacgtt
    13621 cggcagcttg cagcgcccgc tgtattcgcg gcgcgtctgg cgagtccgga ttcaccagct
    13681 ccaggctgcc aataatcacc gccagcagat tattaaaatc atgggccagc ccgcccgtca
    13741 gttgcccgac agctttcagt ttctggctgt gtaatagcgc ctcttccaac ccctgccgct
    13801 cagtttgggc aatctccatc tgcgtggtct tctcttttag cagccgtgag gttcgttcga
    13861 gtgacgcggt attgcgggca aaaacgttaa aggcgcgcgc cagttcaccc aactcatcac
    13921 gccgctgtaa cgccggaaaa gtgacgtccg cttcgccgtg cgccaggcgg ctcatggcgc
    13981 gggaaatcgc cgtcaggtta gtgccgaggt tgcgatagat ataccagccg gcaaacgcgg
    14041 tgatcaccac cgctaacagg gcgaaaatca tgatcaccct ctcgccggag cgcaaatcct
    14101 ggtaactttg cgccacacgc tgaccggaga gcgtcgccac ctgttcggcg taacggttga
    14161 tgtcgccatt gaggatcgcc accagcgctt tgatgtgaaa catataccag ctgattgcca
    14221 gatcgctttg ctccagagcg gcagaaagcg gcgcgagttt acgcatttca tcattgaaat
    14281 cattgaaatc attgagtaac gtattaacca atggcgtatc gctgtgggtc ggcaacgcag
    14341 acattacccc gaccagttga tgaataatcg cctgcggcgc gggggtggca atagcggcaa
    14401 caatcagtcg gttcatctcg ttaagcaaaa tatcatcttg cgcgtgagtg actttttgca
    14461 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgttg cgctccagat
    14521 gccgcctttg cccgcgctcc agcataccgc cgacgctggt ttgcagttcg ttgctacgcg
    14581 tgatgattcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    14641 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    14701 ccagcgctcc caccacctgc gacaatcgca ccgccgccgt tgccacgttt gccgtgtcgc
    14761 gcgccagatt caggctgccg gtcatatcat ccaccgtttg ctgctgcacc agctcctgca
    14821 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    14881 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    14941 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    15001 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    15061 accatgaaca gcacgccagt tctggagatg cgcaatattg ccaaagcctt cggcaaattt
    15121 tatgcactca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    15181 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    15241 agcggcgaga ttctcataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    15301 agcgccggta ttacgctgat ttatcaggag atgcagctgg caccgaatct gtcggtggca
    15361 gaaaatattt ttctcggcag cgagctttcc cacggcgggc tggtgcagcg taaagagatg
    15421 ctagtgcagg cgcaaaaagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    15481 gtcatgacgc tgaccattgc cgagcaacag caggtcgaaa tcgcccgcgc actacatcgc
    15541 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg cgaaactcac
    15601 cgcctgtttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacattagc
    15661 caccgcatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctacg cgacgggcaa
    15721 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    15781 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    15841 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagtagcct gctggtgcgt
    15901 tccggcgaaa ttgttggcct cgccggactg gtgggtgccg gacgttccga actggcgcag
    15961 ttgatcttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg tgaaccggtg
    16021 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    16081 aaagaacaag gcttattcct tgaaatggca gcagccgaaa acatcaccat ggcaaccctg
    16141 gagcgcgatg cccgctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    16201 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    16261 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    16321 ctgctcgatg aacccgcccg cggcgtggac gttggcgcca aaagtgagat ctaccggatc
    16381 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    16441 atagtgggaa tgagcgatcg cgtctatgtg atgcgcgaag gcagcattgc cggtgagtta
    16501 aacggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    16561 cattcccagg cggtaacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    16621 tgagcgatct gatgcaaacg gtcggcatct tgcccatttt gattctgatc gtggcggtat
    16681 ttggctttat cacgccaaac ttttttaccg aaagcaacct gctgaatatt acccgccagg
    16741 catcgatcaa cattgttctg gcggcgggga tgaccttcat cattttgacc ggcgggatcg
    16801 atctttccgt gggttcgatc ctcggcacca cagcggtggc ggcgatggtc gtctccctta
    16861 gccctgagtt cgccatgctg tcgatcccgg cagcgctgat gctcggcctg ctgctggggc
    16921 tgttcaacgg cgcgctggtg gcctttgccg gattgccacc gtttatcgtt acgcttggca
    16981 cctatacggc gctgcgcggg gcggcgtatc tgctggcgga cggcaccacg gttatcaact
    17041 ccaacattaa tttcgagtgg ataggcaaca actatctcgg gccggtccca tggctggtgg
    17101 tgattgcgct ggcggtgatc gtggtgtgct ggtttattct gcgccgcacc acgcttggcg
    17161 ttcatatcta cgccgtcggc ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    17221 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttctgggctt ggtgggatta
    17281 tgagcgcctc acggctgtac agcgccaacg gcaacttagg catgggttac gaactggatg
    17341 ccattgcagc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    17401 gcacgctggt cggcgcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    17461 tctcttactt ctggcaactg gtgatcaaag gggcgatgat catcattgcg gtgctgatcg
    17521 acaaataccg tacccgacac catcaaagtg cataaacaca tgaggaatac agcatgagat
    17581 tgaagccctt agtgaccgcg ctctgtgcag gcgcgctgct ggccgcagca ccgttcgccc
    17641 aggcgaaaga actgaaatca ataggcgtaa cggtgggcga tctggcgaac ccgttcttcg
    17701 tgcagatcac caaaggcgcg gagctggaag cacgcaaact ggcaggcgat aacgtcaaag
    17761 tgacgctggt ttccagcggc tacgatctgg gtcagcaggt gtcgcagata gacaacttta
    17821 tcgccgctaa cgtcgatatg attatcctca acgctgccga ttcaaaaggg atcggcccgg
    17881 cggtgaaacg ggcgaaagat gccgggatcg tggtcgtcgc ggttgacgta gcggcagaag
    17941 gtgccgatgc caccatcact tccgataaca cccaggcagg cgaaatggcc tgtaaataca
    18001 tcaccgatcg cctgaaaggc aaaggcaacg tggtgatcat caacggcccg ccggtatccg
    18061 ccgtgcaaaa ccgcgtcgaa ggatgccaga ctgaatttaa acgccatcct gacatcaaag
    18121 tgctctctga caaccagaac gcgaaaggca gtcgtgaagg cgggctggag gtaatgacgg
    18181 gactgctggc ggcaaatccg aagatcgacg gcgtatttgc gattaacgat cccaccgcaa
    18241 tcggtgccga tctggcagca aaacaggcac aacgtaacga gttctttatt gtcggtgtgg
    18301 acggaagccc ggacgccgaa gaggcgctca agcgtgagaa ctcgttgttt gtcgctactc
    18361 cggcgcaaga tccccaggtg atggcagcga aagcggtgga aattggctac gacattttgc
    18421 agggcaaacc tgcgccgaaa gagccggtgc tgatcccggt gacgatgatc gataaaaaca
    18481 acgtcggcaa ttacaaaggc tggacggtga aataagtcca cacgccgggc ggcgcagctg
    18541 cccggtcacc gctaaggaga acgctttcga tgaaacgctc cgctataaac gacattctcg
    18601 gccatacacg gcaattcttt tcgcaacatg acgtacatct gccgcccttt gccagtttct
    18661 cgcctgctca atggcagcag ctcgacaccg ccgcgtggga ggaggttttt gatcttaaac
    18721 tcggctggga tgttaccgct tttggtcgca acaactttgc agcacacggg ctgacgctgt
    18781 ttaccctacg taacggctca gcaaaaggaa tgccgtatgt gaaatgttac gccgagaaga
    18841 tcatgcatgt gcgcgatgcg caggtcacgc caatgcattt tcactggcgt aagcgtgagg
    18901 acattatcaa tcgcggcggc ggtaatttaa ttgttgaact gtggaatgcc gatagtaacg
    18961 agcaaacggc tgatagtgac attacggtgg tgatagacgg ctgccgccag aaacatactg
    19021 caggctctca gttgcgcctg tctcccggcg aaagtatctg cctgccgccc ggcctgtatc
    19081 acagcttttg ggcagaagcc ggttttggcg atgtgctggt tggcgaagtc tcttctgtta
    19141 atgacgacga ccacgataac cactttttac agccactgga ccgctacaac ctgattgacg
    19201 aagacgaacc tgcacagttg gtattgtgta acgaataccg ccagttccgc tgaataagga
    19261 taacgttatg ccattgattt ctctcgctga agggctggca catgcccgcg aacatcacta
    19321 ttcgttaggt gcgtttaatg tgctggactc gcacttttta cgcgccttgt ttgctgctgc
    19381 cagacaagaa cgttcgccct ttattatcaa cattgccgaa gtacatttta aatatatctc
    19441 gctggattcc ctggtcgaga cagtgaaatt tgaagcggcg cgtcatgaca ttccggtggt
    19501 gttaaatctc gatcacggac tgcattttga atcggtggtg caagcgttgc ggctgggatt
    19561 cagctcggta atgtttgatg gctccacgct ggggtatgaa gaaaatgtcc gccagacgcg
    19621 ggaagtggtg aaaatgtgcc atgccgttgg cgtatcggta gaagctgaac tgggcgcggt
    19681 gggtggcgac gaaggcggtg cactctatgg tcatgccgat gaatccctgt ttaccgatcc
    19741 gcaactggcg cgtgattttg tcgaccgtac cggcattgac gcgctggccg tggcgattgg
    19801 caatgcacac ggcaaatatc gcggcgaacc gaaactcgat ttcccgcgcc tcgacgctat
    19861 ccgccagcag gctgctatcc cgctggtatt acacggcggc tcagggatta gcgatgccga
    19921 tttccgccgc gccatcgaac tgggtatcca caaaatcaat ttctacactg gcatgtcgca
    19981 agcggcgctg gctgccgttg aggcccgaat ggcgcaccgt cagccgatgt atgacgagtt
    20041 tgccgagtta ctgctggcag tggaagccgc catcagcgat acggttgcac aacagatgcg
    20101 tatttttggc agtgcggggc agacatgatg gagcgtaaag ggattatcgc cgcaggcaat
    20161 atgttggttg accacgtcca tcagattatc cagtggccag agcgcggctg gctggcggag
    20221 atcacccaca gcgaacgctc caccggcggt gcgccgctta acgtcctgct gacgctggcg
    20281 aaaatgcgca ccgggctgcc gttacaggcg gttgggctaa tcggtcagga cagcgacggc
    20341 gattacatca tggcgatgct tgagcaatat cacgtcaatc gtcagcatgt gcagcgcact
    20401 acctttgccc cgacgtcgat gtcgcaggtg atgacagacc caagcgggca gcggacgttt
    20461 ttccattcgc cgggtgcaaa cagacttttg gatcttccgg cgttcgatca gctcgataac
    20521 accatgaaga ttttccatct cggctattta ctgttgctcg acagcctgga tatgccggac
    20581 gatgtttacg gcacccgtag cgcacgcctg ctggcgcaaa tgcgtgatac tggctttgaa
    20641 acctcactcg acctggtctc ccgtaagggc gatccgcgtt atcagccgct ggtgctgccc
    20701 gcgttgcgcc atctggatta tctggtaatt aacgaactgg aggccgggga gtttagcggg
    20761 ctggaaattc ggttacccaa cggcgaaccg catatcgcgc atattgctgc cgccgcgcgt
    20821 gaactgttag atgcgggcgt gcgccagcgg gtggtgatcc actgcccgga aggggcgtgg
    20881 ggagagaccc cagaacaggg cggcgtgtgg atcccttcgc aaaagttaga acaacgggaa
    20941 attatcggca gcgtcggcgc gggcgatgca ttttgtgccg gatttttata tggctgccat
    21001 gaacgctgga cgcttaccga gagcatcaaa ctggcacacg cctgcgcgcg ggcaagcctg
    21061 ttatgcgcca atgccatcga cggggcgaaa acgctggaag agctgcaaac agaaatgtcg
    21121 gattaatgca cgtcggcagc gaagacatac cccagaccgc gcagagtttt aatcaacatc
    21181 ggctgatgcg ggttgagttc gattttgcgt cgtaagcgca tgattaatac gtcgatggtg
    21241 cgatcaaaca cctccacgct gtcgctgtgg gtgaacgcca gcaactgctc gcggctcaat
    21301 acccgacgcg cgttttgcgc cagagccagt aacaagccat attcgccctg agttaactcg
    21361 acatgttgct gaagcggatt gtgcaactgg cagcgcatgg tatcgagata ccagccattg
    21421 aatgacagcc cggcgctttt ctcgtcatta accgccagcg cgccaacccg tcgcaacacc
    21481 gctttcagtc gtgcgaccac cacccgcggg gtgaacggtt tgcagatgta atcatccgcg
    21541 cccatttcca gcccgaccac cacatcagac tcactgccca tgccggtcaa catcaccacc
    21601 ggcaatgtcg gacgcagttt ctggatttgc tgcaataccc tcaggccgtt ggtatcgggt
    21661 agcatcatat ccagcaccac cagcgcaatg tcaggatcgc cctcaatacg caatatggcc
    21721 tcctgtccgg tatggcagac tgaaaccgta aagacatgtt cacttaacac gtcctgcaac
    21781 agtgcgcaga tagccgtgtc atcatccacc accagcacga cgggtttcat ggttacactc
    21841 cgcatcattc atctgattaa tcattgtgcg tacttcttgt cgaagtgaaa gctgaaagta
    21901 atataaataa taaaaatata agtcaggtca cattattgat gatagtaata aatatgtcgt
    21961 acttttccct cttcagcaca aatgcctaat aaaatggatc gttccgctca cacttccact
    22021 tattaaagtc gcaatttccc aattatgata acgccagctt taaatgacgt attttgcatg
    22081 gattacatat ttacgacata aatgataaat ggagatttat atgaataact ctcggttatt
    22141 ccgtttgagc aggattgtta ttgcgttaac tgccgccagc ggcatgatgg taaataccgc
    22201 taacgcgaaa gaggaagcga aagccgccac tcaatatacc caacaggtta atcagaatta
    22261 cgccaaatca ttaccgttta gcgatcgtca ggattttgac gatgcccagc gtggatttat
    22321 cgccccgctg ctggatgaag gtattctgcg tgatgcgaac ggtaaagttt actaccgcgc
    22381 agacgattac aaatttgata ttaatgccgc tgcgccggaa accgtaaacc ccagcctgtg
    22441 gcgtcagtcg cagatcaacg gcatttctgg cctgttcaaa gtcaccgata aaatgtatca
    22501 ggtgcgcggc caggatatct ctaacattac gttcgttgag ggggagaaag gcattattgt
    22561 catcgacccg ctggtgacgc cgcctgccgc aaaagccgca cttgaccttt acttccagca
    22621 tcgtccgcaa aaaccgattg ttgccgttat ctacactcac agccacaccg accactatgg
    22681 tggcgtgaaa ggcattatct ctgaagccga tgttaaatcc ggcaaagttc aggtgattgc
    22741 ccctgctggc tttatggacg aagccatcag cgaaaacgtg ctggcgggta acatcatgag
    22801 ccgccgtgcg ctctactctt acggtctgtt actgccgcac aacgcgcaag gcaacgtggg
    22861 taatggcctt ggcgtgacgc tggcaacggg cgacccgagc attattgcac cgactaaaac
    22921 tatcgtcaga actggcgaga agatgattat cgacggcctg gagtttgact tcctgatgac
    22981 cccaggtagc gaagcgccag ccgaaatgca cttctatatt ccggccctga aagccctgtg
    23041 taccgccgag aacgccacgc ataccctgca caacttctac actctgcgcg gcgcgaaaac
    23101 ccgcgatacc agcaagtgga ccgagtacct gaacgaaacg ctggatatgt ggggtaacga
    23161 cgcggaagta ctgtttatgc cgcacacatg gccggtctgg ggcaataagc atatcaatga
    23221 ttatattggt aaatatcgcg atactatcac gttcattcac gaccagaccc tgcacctggc
    23281 gaaccagggc tacaccatga atgaaatcgg cgacatgatt aagctgccgc ctgcacttgc
    23341 caataactgg gccagccgtg gctattacgg ttctgtcagc cacaacgccc gcgcggtgta
    23401 taacttctat cttggctatt acgacggtaa cccggctaac ctgcatccgt atggtcaggt
    23461 agagatgggc aaacgttacg tgaaagcgct gggcggctct gcccgtgtca tcaacctggc
    23521 gcaagaagcg aataagcaag gtgattaccg ctggtcggca gaactactga aacaggtgat
    23581 tgccgccaac ccgggtgacc aggtggcgaa gaatctgcaa gcgaataact ttgaacagct
    23641 gggctatcag gccgagtccg ccacatggcg cggtttctac ctgaccggcg cgaaagagct
    23701 gcgcgaaggg gtgcataagt tcagccacgg caccaccggt tccccggaca ccattcgcgg
    23761 aatgtcggtc gaaatgctgt tcgactttat ggccgttcgc ctcgatagcg cgaaagctgc
    23821 gggtaaaaat atcagcctga acttcaatat gagcaacggc gataacctca acctgacgct
    23881 gaacgatagc gtgcttaact accgtaaaac actgcaaccg caagccgacg cctctttcta
    23941 catcagccgt gaagatctgc acgccgtgct gaccggacag gcaaaaatgg cggatctggt
    24001 gaaagcgaag aaagccaaaa ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg
    24061 tctggataat ttcgatttgt gggtgaatat cgtaacccca aattaatgtc actcgctttt
//
LOCUS       DAZGXS010000068        24112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGXS010000068 DAZGXS010000000
VERSION     DAZGXS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            142..3894
                     /gene="yfaL"
                     /locus_tag="ACHKG5_002792"
     CDS             142..3894
                     /gene="yfaL"
                     /locus_tag="ACHKG5_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0868863.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4035..4757)
                     /gene="ubiG"
                     /locus_tag="ACHKG5_002793"
     CDS             complement(4035..4757)
                     /gene="ubiG"
                     /locus_tag="ACHKG5_002793"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0868864.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4904..7531
                     /gene="gyrA"
                     /locus_tag="ACHKG5_002794"
     CDS             4904..7531
                     /gene="gyrA"
                     /locus_tag="ACHKG5_002794"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0868865.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7739..9367
                     /gene="yfaA"
                     /locus_tag="ACHKG5_002795"
     CDS             7739..9367
                     /gene="yfaA"
                     /locus_tag="ACHKG5_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP0868866.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9364..9987
                     /gene="yfaT"
                     /locus_tag="ACHKG5_002796"
     CDS             9364..9987
                     /gene="yfaT"
                     /locus_tag="ACHKG5_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP0868867.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10011..14525
                     /locus_tag="ACHKG5_002797"
     CDS             10011..14525
                     /locus_tag="ACHKG5_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0868868.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14526..16175
                     /gene="yfaQ"
                     /locus_tag="ACHKG5_002798"
                     /pseudo
     CDS             14526..16175
                     /gene="yfaQ"
                     /locus_tag="ACHKG5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16180..16956
                     /gene="yfaP"
                     /locus_tag="ACHKG5_002799"
     CDS             16180..16956
                     /gene="yfaP"
                     /locus_tag="ACHKG5_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0868869.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17231..20080
                     /gene="rcsC"
                     /locus_tag="ACHKG5_002800"
     CDS             17231..20080
                     /gene="rcsC"
                     /locus_tag="ACHKG5_002800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0868870.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20280..20930)
                     /gene="rcsB"
                     /locus_tag="ACHKG5_002801"
     CDS             complement(20280..20930)
                     /gene="rcsB"
                     /locus_tag="ACHKG5_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0868871.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20947..23619)
                     /gene="rcsD"
                     /locus_tag="ACHKG5_002802"
     CDS             complement(20947..23619)
                     /gene="rcsD"
                     /locus_tag="ACHKG5_002802"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0868872.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVSG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5907 a         5859 c         6553 g         5793 t
ORIGIN      
        1 attatgacgc actgatacta ttcatcaaat aataacaaaa tagctattgc atcgggttgg
       61 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
      121 taaaatgatg gtcagattaa tatgcgggtt atctttctac gcaaggagta tttatctcta
      181 ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta
      241 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
      301 acgcaggtct ggagtattgc cgatgggcaa tggctggttt tttcggatat gaccaataat
      361 gccagcggtg gggccgtatt tttgcaacaa ggagcggaat ttacattatc accagaaaat
      421 gaaactggaa tgactctgtt tgccaataac accgtttcag gagaatataa taacggcggg
      481 gcaatatttg ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt ttctggtaac
      541 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac cggtgccatc
      601 gatttacgtg tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg caaaggtggt
      661 gcaatttata ccatcaataa tgatatctat ttaagtgatg atgtttttaa caataaccag
      721 gcatatacat caacaagtta cagtgatggc gatggcggcg caatcgatgt cacagataat
      781 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat
      841 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
      901 attgatattt ctgttgatga cagctacagc cagaacggag gcgtgttagt cgatgagaac
      961 aatagcgcag caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc
     1021 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
     1081 gagaatgacg gagctgttga ctctattgct ggtaccgggt taatcaccaa aacaggttcc
     1141 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
     1201 ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
     1261 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
     1321 gcagagctaa atgttggctc cacccaacaa acctttgcgc actcattgac gggctttcag
     1381 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
     1441 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta tgtgctggcg
     1501 ggggcgcagt cgatggcgct aaccggcgat atagtggtgg atgctggtgc ggtgctttcg
     1561 ctggaaggcg acgcggcaga tcttgccgct ctccaggacg atccgcagtc gatcgtgtta
     1621 aacggcggta tgctcgatct ctctgatttc tccacctggc agagcggtac atcatacaaa
     1681 gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
     1741 cttgcaggcg gaaacgatat gcatatcggc ggcgacggga aagatggcgt ctacgtggtg
     1801 atcgatgcgg gtgacgggca ggtcagcctg gcaaatgaca atcaatacct cggcacaacg
     1861 caaatcgctt ccggtacgct gatggtgagc gacaactcgc agcttggata tacccattat
     1921 aaccgccagg ttatctttac cgataagcca caagaaagcg tgatggagat tactgccaat
     1981 gtcgatactc gctctacaac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
     2041 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
     2101 gggcagcatc aggatgaggg tagcacattg actaaaacgg gggcgggtac actggagctg
     2161 accgccagcg gtacaacgca gtcagcggtg agagtagaag agggcacgct gcaaggtgat
     2221 gttgcggata tcttccctta tgcttcgtcg ctatgggtcg gtgacggggc aacgttcgtt
     2281 actggcgcgg atcaggatat tcagtcaatt gatgctactt ccagcggcac tatcgacatc
     2341 agcgatggta cggttttgcg cctgaccggg caggatactt ccgtcgccct taatgcctca
     2401 ctgtttaact gcgatgggac gctggtgaat gccaccgatg gtgtgacgtt gacaggtgag
     2461 cttaatacca accttgaaac tgacagcctg acttatcttt ccaacgtgac ggttaatggc
     2521 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
     2581 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
     2641 gatgactcgg taagcgatca attggtgatg aacggtaata ctgctggcaa cacaactgtg
     2701 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
     2761 ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagcggc
     2821 tacgtcaata tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg
     2881 cgatcgcaag aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat
     2941 cccacgcagg atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg
     3001 ttgaatgcca aagttggcgg ttatctcaat aacctgcggg cggcaaatca ggcgtttatg
     3061 atggagcgac gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc
     3121 ggagattatc attacacagc agcggggcaa ctggctcaac atgaagacac ttctacggtg
     3181 caacttagcg gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg gatgcttggg
     3241 attgttggtg gctacagcga taaccagggc gacagccgct cgagtatgac cggaactcgc
     3301 gccgataacc agaaccacgg ttatgcggtt gggctgacct caagctggtt tcagcacggt
     3361 aagcagaagc agggggcctg gctggataac tggttgcagt acgcgtggtt tagcaatgat
     3421 gtttctgaac atgaagatgg cgtggatcat taccattcgt cggggattat cgcctcgctg
     3481 gaagcggggt atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg
     3541 atttatcagg gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa
     3601 tcgcagggtg atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt
     3661 catgtcatac caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa
     3721 gaagatgcca gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga
     3781 gtcacgggca atatcagtca gcgagtttcg ctgcgtggta gcgtggcgtg gcagaaaggg
     3841 agtgatgatt ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg gtaatcgctg
     3901 tctgaccatc actattgctg gatgcggcgt aaacgcctaa tcaggcctac ggagagcatt
     3961 tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt gccggatgaa
     4021 tccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc cacgccgggg
     4081 ccgagtttaa aagtattagt gatcgggttg taatgtagcc cggttatatg tcgctcttta
     4141 aggcttgtct gatctaccca gcccagcagt tctgccggtt taataaactt cttcacatca
     4201 tgtgtacctt taggcaccat gcgcaaaata tattccgcgc cgactaccgc catcagccat
     4261 gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt caccagttgc
     4321 gcacaggctc tgaccactga ctgcggatca gggacgtgct ccagcatctc catgcaggtc
     4381 accacatcat actgcccggc atgttttgcc gcgtgctctt ccacggtttc ctgcacgtaa
     4441 tccacctgaa tgccgctttc cagcgcgtgc agctttgcca cctgcaatgg ctcaaagccc
     4501 atatccagac cggtcaccgt cgcgccttcg cgcgccatac tctcggccag aatgccgccg
     4561 ccacaaccga catcgagcac ctttttgcca aataagccgc cagcacgctc ggcaatatag
     4621 ctcagacgca gcgggttaat gcggtgcagc ggtttgaact caccttccag atcccaccag
     4681 cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg gtttaccggc
     4741 gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac gagtatatca
     4801 ggcattggat gtgaataaag cgtataggtt tacctcaatc tgcgcggctg tgttataatt
     4861 tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg accttgcgag
     4921 agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg attatgcgat
     4981 gtcggtcatt gttggccgtg cgctgccaga tgtccgagat ggcctgaagc cggtacaccg
     5041 tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct ataaaaaatc
     5101 tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact cggcagttta
     5161 tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg tagacggtca
     5221 gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata cggaaatccg
     5281 tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg tcgatttcgt
     5341 tgataactat gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa ttcctaacct
     5401 gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc cgccgcacaa
     5461 cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca tcagcattga
     5521 agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca ttaacggtcg
     5581 tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtatatatcc gcgcccgtgc
     5641 tgaagtggaa gttgacgcca aaaccggacg tgaaaccatt atcgtccacg aaattccgta
     5701 tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag aaaaacgcgt
     5761 ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca tcgtgattga
     5821 agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac ctctactccc agacccagtt
     5881 gcaggtttct ttcggtatca acatggtggc attgcaccat ggtcagccaa agatcatgaa
     5941 cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga cccgtcgtac
     6001 tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat tagccgtggc
     6061 gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc ctgcagaagc
     6121 gaaaactgcg ctggtggcta atccgtggca gctgggcaac gttgccgcga tgctcgaacg
     6181 tgctggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg tgcgtgatgg
     6241 tctgtactac ctgaccgaac agcaagctca ggcaattctg gatctgcgtt tgcagaaact
     6301 gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg atcagatcgc
     6361 ggaactgctg cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc gtgaagagct
     6421 ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg ccaacagcgc
     6481 agacatcaac ctggaagatc tgatcaccca ggaagatgtg gtcgtgacgc tctctcacca
     6541 gggctacgtt aaatatcagc cgctttctga atacgaagcg cagcgtcgtg gcgggaaagg
     6601 taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg tggcgaacac
     6661 gcacgaccat attctgtgct tctccagccg tggtcgcgtc tattcgatga aagtttacca
     6721 gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc tgccgctgga
     6781 gcaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag gcgtgaaagt
     6841 cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc ctcaccgagt tcaaccgtct
     6901 gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc gatgagctga tcggcgttga
     6961 cctgaccagc ggcgaagacg aagtaatgct gttctccgcc gaaggtaaag tggtgcgctt
     7021 taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg gtattcgttt
     7081 aggcgaaggt gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg caatcctcac
     7141 cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg gaatacccga ctaagtcgcg
     7201 tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt aacggtttag ttgttggcgc
     7261 ggtacaggta gatgactgcg accagatcat gatgatcacc gatgccggta cgctggtacg
     7321 tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc cagggcgtga tcctcatccg
     7381 tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt gctgaaccgg ttgacgagga
     7441 agatctggat accatcgacg gcagtgccgc ggaaggggac gatgaaatcg ctccggaagt
     7501 ggacgttgac gacgagccag aagaagaata attttacttc ttcatgccaa aagggagcca
     7561 tctccctttt ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt cgtattaggg
     7621 cgcggtaaag tttggctgtg tccgtaaaaa atggctggct atacacaagg aatgtggcaa
     7681 tgagtggtga aaaaaagcga aaggctggcg gttctatggt cttgtaggtt ttggcgcaat
     7741 agcactgctt tccgctggcg tctgggcgtt gcaatatgct ggcagtgggc cagaaaaaac
     7801 gttgtcgccg ctggtggtgc acaataattt gcaaattgat ctcaatgagc cggacctctt
     7861 tctcgacagc gattctctaa gccagcttcc caaagatctc ctcaccattc cgtttctcca
     7921 cgatgttctg agtgaagatt tcgttttcta ttatcagaat catgccgatc gtctgggcat
     7981 tgaaggcagc attcgtcgca ttgtctatga acacgatctc acgctgaaag ataagctctt
     8041 ttcgtcactc ttagatcagc ccgcgcaggc ggcgctatgg cacgataaac aaggccatct
     8101 ttcacattac atggtgctga tccaacgcag tggtttaagc aaactgctgg agccattgtt
     8161 atttgccgct accagcgaca gccagttaag caaaacggaa atcagtagca tcaagataaa
     8221 tagtgaaact attcctgttt atcagttgcg ctataacggc aataacgccc tgatgttcgc
     8281 gacatatcag gacaagatgc tggtgttttc cagcacggat atgctgttta aagatgatca
     8341 gcaggatacc gaagccacgg cgatcgcaag tgatttgttg agcggcaaaa aacgctggca
     8401 agccagcttt ggcctggaag agcgtgctgc cgaaaaaacg ccagtacgcc agcgcatcgt
     8461 ggccagcgcc aggttgctgg gttttggcta ccagcggtta atgccttctt ttgctggcgt
     8521 acgcttcgaa atgggtaacg acggctggca cagttttgtg gcgttaaatg atgaatccgc
     8581 cagcgtagat gccagtttcg attttacgcc ggtctggaac agtatgcctg ccggggccag
     8641 cttctgtgtg gcggtgccgt attcacacgg tattgccgaa gagatgcttt cgcacatcag
     8701 ccaggaaaac gacaagttga atggggcgtt ggacggtgcc gcggggctgt gctggtatga
     8761 agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt gatggcactg ccgaacaggc
     8821 gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg cacgaaagca aagcgccaga
     8881 aggtgttttg ccggtaagcc agactcagca gggcgaagcg caaatctggc gtcgcgaagt
     8941 gagttcccga tacggtcagt atccgaaagc gcaggcggcg caacccgatc aattaatgtc
     9001 ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa acgctgcttt tctccctcga
     9061 tgacacgctg gttaataacg ccctgcaaac actgaataaa acccgcccgg caatggtgga
     9121 tgtaataccc actgatggca tcgttccgct ctatatcaat ccacaaggcg tggcgaaact
     9181 gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc gaaccggttt tttataacgc
     9241 cgcacaaact ttattaatgc cgaagctgga cgctttatct caacaaccgc gttatgtcat
     9301 gaagctggcc cagatggaac ccggtgccgc ctggcagtgg ctacccataa tctggcagcc
     9361 gctatgaggc acgggctgct ggcgctgatt tgctggctgt gttgtgttgt tgcccatagc
     9421 gaaatgctga atgtcgaaca atccgggctg tttcgcgcct ggtttgtacg cattgcacaa
     9481 gaacagctcc gccaggggcc aagtccacgc tggtatcagc aggattgtgc gggcctggtg
     9541 cgatttgcgg cgaacgagac gctgaaagtt cacgacagta aatggctaaa aagtaacggt
     9601 ttatctagcc agtatttgcc gccagagatg acgctaacac ctgaacagcg tcaactggcg
     9661 caaaactgga atcaggggaa cgggaaaacc ggcccctacg tgaccgcgat taatttgatt
     9721 cagtacaaca gccagtttat tggccaggac ataaaccagg cgctgcctgg cgatatgatt
     9781 tttttcgatc agggcgatgc ccagcactta atggtctgga tggggcgtta cgtcatctac
     9841 cacaccggaa gcgccacgaa aactgacaac ggaatgcgcg cagtcagtct gcaacaactt
     9901 atgacatgga aggacacccg atggataccc aacgattcca atcccaattt cattggcatt
     9961 tatcgtttaa attttctggc gcgatagcag cgtgcttgtc cctgtctctt gtgggaacag
    10021 ggcttgctaa tgctgatgat tcgcttcctt ccagtaacta tgcgccgccc gccgggggaa
    10081 cattcttttt gcttgctgac agcagtttta gcagcagtga agagtcgaaa gtgcgactgg
    10141 aagcgccggg gcgtgattat cggcgctatc agatggaaga gtacggcggc gtggacgttc
    10201 gcctgtatcg tattcctgac ccgatggcat ttttgcgcca gcagaaaaac ctgcatcgca
    10261 ttgtggtgca accgcaatat ctgggcgacg ggctgaacaa tacgctgacc tggctgtggg
    10321 ataactggta cggcaaatct cgccgcgtga tgcagcgtac tttctcttct cagtcacggc
    10381 agaatgtgac tcaggcatta cccgaattac agctcggcaa tgccattatt aaaccttccc
    10441 gttatgtaca gaacaaccag ttttccccgc tgaaaaaata tcccctggtg gaacagttcc
    10501 gttatccact atggcaggct aaaccggtcg agccgcagca aggggtaaaa ctggaaggcg
    10561 catccagcaa tttcatctcg ccgcagccgg gtaacattta tattcctctc ggccaacaag
    10621 agccgggact gtacctcgtc gaggcgatgg ttggtgggta tcgggcgacg acggtggtgt
    10681 ttgtttccga taccgtggcg cttagcaaag tgtcaggcaa agagcttctg gtgtggaccg
    10741 cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat cttgtggact gacggtcttg
    10801 gcgtgatgac ccgcggtgtg accgatgaca gcggtacctt gcagttacaa catatatcgc
    10861 cagaacgttc atacattctg ggtaaggatg ctgaaggcgg cgtttttgtc tccgagaact
    10921 tcttctacga aagcgaaatc tacaacaccc gcttgtatat ttttaccgat cgcccgctat
    10981 atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg cgagttccac gatccgttgc
    11041 attcatcccc catcgtcagc gccccggcga agctttcggt gctggacgct aacggcagtc
    11101 tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg cgggcaggga agtttccgcc
    11161 tgccagaaaa tgccgttgcc ggaggttatg agttacgtct tgcttaccgc aatcaggtct
    11221 atagcagcag ttttcgcgtg gcaaactaca tcaagccaca tttcgagatt ggtttagctc
    11281 tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg caaactgcaa cttctctacc
    11341 cggatggcga gccggtaaaa aatgcccgcg tgcagttaag tttgcgcgct cagcaattat
    11401 caatggtcgg taacgatttg cgttatgccg gacgtttccc cgtgtcgctg gaaggcagcg
    11461 aaacggtgtc cgacgccagc ggtcatgtgg cgttaaatct ccccgccgcc gataaaccga
    11521 gccgctattt gttaaccgtc tccgccagtg acggcgcggc gtatcgcgtc accaccacca
    11581 aagagatcct cattgaacgc ggcctggcgc attactcttt aagtaccgcc gcacaataca
    11641 gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct ggaatcttca aaacaggttc
    11701 ctgttacgta tgaatggttg cgtctcgaag accgcacgag ccatagcgga gatctaccgt
    11761 caggcggcaa atcctttacc gtcaatttcg ataaacctgg caactacaat ctgacgttac
    11821 gcgataaaga cggcttaatt ctcgccgggt taagccatgc cgtcagcggt aagggcagta
    11881 tgtcgcatac tggtacggta gatatcgtgg cagataaaac gctgtaccag cctggcgaaa
    11941 ccgcgaagat gctgattacc ttcccggagc caattgatga agcattattg acgctggaac
    12001 gcgatcgcgt tgaacagcag tcgctgctat cgcatccggc aaactggctg acgttacaac
    12061 gtttaaacga tactcaatat gaagcccgtg ttccagtgag caattccttt gcgcctaaca
    12121 tcactttttc ggtgctgtat acccgtaacg gtcagtacag ttttcagaac gccgggatca
    12181 aagttgccgt tccccagctg gatatccggg tgaaaacgga caaaacccat taccagcctg
    12241 gtgaactggt caatgtcgaa ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc
    12301 taacggtagg cgtggtcgat gaaatgatct acgcgctgca accagaaatc gcgccgaata
    12361 tcggcaaatt tttctatccg ctggggcgta acaatgtgcg taccagctcc agtctgtcgt
    12421 ttatcagcta cgaccaggca ctctccagcg agccggttgc gcctggcgca actaaccgca
    12481 gcgagcggcg agtaaaaatg cttgaacgtc cacggcgtga agaggtggat accgcggcat
    12541 ggatgccgtc actcacaacc gataaacaag gcaaagcgta tttcacgttc ctgatgcctg
    12601 attcgttaac ccgctggcgt atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc
    12661 aggggcgtgc ttatctgcgt tcggaaaaaa atctctacat gaagtggagt atgccaacgg
    12721 tgtatcgcgt gggcgacaaa ccgtcggcag gactgtttat cttcagtcag caggataacg
    12781 aaccggtggc gctggtgact aaatttgcag gcgctgagat gcgccagacg ctgacgctgc
    12841 acaaaggggc gaattatatt tcgctggcgc agaacattca gcaatctggc ttgttaagcg
    12901 cagaactgca acaaaatggg caagtgcagg acagcattag cacaaaactg tcttttgtgg
    12961 ataacagctg gcccgttgaa cagcagaaaa atgtcatgct cggcggtggc gataacgcgc
    13021 tgatgttgcc cgagcaggcg agcaatatcc ggctacaaag tagtgaaacg ccgcaggaga
    13081 ttttccgcaa caatcttgat gcgttagtcg atgaaccgtg ggggggggtg atcaacaccg
    13141 gtagccgtct gatcccgctc agtctcgcct ggcgttcgct tgccgatcat caaagtgccg
    13201 ccgctaacga cattcgtcag atgattcagg ataaccgtct gcggctgatg caactggcgg
    13261 ggcccggagc gcgctttacc tggtggggtg aagatggcaa tggtgacgcc ttccttacgg
    13321 catgggcatg gtacgccgac tggcaggcca gccaggcgct cggcgtaacg caacaaccgg
    13381 aatactggca gcatatgctc gacagttatg ccgagcaggc agataacatg ccgttattgc
    13441 atcgggcgct ggtgctggcg tgggcacagg agatgaatct gccgtgcaaa acgttgttga
    13501 aagggttgga tgaagctatc gcccggcgcg gaactaaaac tgaagatttc tctgaggaag
    13561 acacccgcga tatcaatgac agcctgatcc tcgatacacc ggaatctcca ctggcagatg
    13621 cggtggcaaa cgtcttaacc atgacgttgc tgaaaaaagc gcagttgaag tccacggtga
    13681 tgccacaggt tcagcaatat gcgtgggata aagcggtaaa cagcaatcag ccgctggcac
    13741 acacggttgt gctgctcaat agcgggggcg acgctaccca ggcggctgct attttaagtg
    13801 gtttgaccgc tgagcaatct actattgagc gcgcgctggc catgaactgg ctggcgaaat
    13861 atatggcgac aatgccttcg gttgtgttgc ctgcgcctgc gggcgcatgg gccaaacata
    13921 agttaactgg agggggcgaa tactggcgtt gggttggtca gggcgtgccg gacattctct
    13981 cttttggtga cgaattatcg ccgcaaaatg tgcaggtccg ctggcgtgaa gcggcaaaaa
    14041 cggctcaaca aagtaacatt ccggtgaccg ttgaacgcca attgtatcgg cttatccctg
    14101 gtgaagaaga gatgagcttt actctgcaac cggtgaccag caatgagatt gacagcgatg
    14161 cgctgtatct cgatgaaatt acgcttacca gcgagcagga tgcagttctg cgctatggtc
    14221 aggtggaagt accgctcccg ccgggagccg acgttgagcg cacaacatgg ggcatttcag
    14281 tcaataaacc caacgctgga aaacagcagg ggcaattgct ggaaaaagcg cgaaatgaaa
    14341 tgggcgaact ggcctatatg gtgccggtga aagaactgac gggaacggtc actttccgcc
    14401 atttgctgcg cttctcgcaa aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt
    14461 cctatgcacc tgcacagcaa agtgttgcgg cagggagcga atggaccggg atgcaggtga
    14521 aataagtgaa ctggcgcaga atcgtctggc tattggcgtt ggttactctg ccaacgcttg
    14581 cggaggagac gccgttacaa ctggcgctgc gcggtgcgca gcacgatcaa ctgtatcagc
    14641 tgtcgtcatc aggcgtgacc aaagtctcag cattacctga cacgctcacc acgccgttag
    14701 ggagtttatg gaaactgtat gtttacgcct ggctggagga tacccatcaa ccggagcagc
    14761 cctaccagtg tcgcggaaac tccccggaag aggtttattg ttgtcaggct ggggagagca
    14821 taacgcgcga taccgcgctg gtacgttcct gtgggctcta ttttgcgccg cagcggttgc
    14881 atattggggc agatgtgtgg gggcaatact ggcaacagcg tcaggcacct gcgtggttag
    14941 cgtctctttc tacgctgaag ccagaaacct cggtaacggt taaatcgctg cttgattcat
    15001 tagcgacgtt acctgcgcaa aacaaagctc aggaagtctt actggatgtg gtgctggacg
    15061 aggcgaaaat cggtgtcgca tctatgctcg gtagtcgggt acgggtgaaa acctggagct
    15121 ggtttgccga tgataaacag gaaatccgtc agggtggttt tgctggctgg ttaaccgatg
    15181 gtacaccgct gtgggtgact ggcagtggca ccagcaaaac ggtactgacc cgttatgcga
    15241 cggtgcttaa tcgggttttg ccagtgccga cgcaagttgc cagtgggcaa tgtgtagagg
    15301 tggagctatt tgcgcgctag ccgctgaaga aaatcacagc ggagaagagt acgacggcag
    15361 taaaacccgg cgtacttaat gggcgctatc gtgtgacgtt tgccaatggt aaccacatta
    15421 cgtttgtcag ccacggtgag acgaccttac tgaccgaaaa gggcaaactg aaattgcagt
    15481 cgcatctcga tcgtgaagag tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc
    15541 cacctgaggc agcgaaagcc atgactgtgg cgatacgcac ttttttacag caaaacgcca
    15601 atcgggaagg cgattgcctg accatccccg acagcagcgc cacgcagcgt gtttctgctt
    15661 cgccagctac cacgggggcg cgaacgatga ccgtctggac gcaggatctc atctatgcgg
    15721 gcgatccggt tcattatcac ggcagtcgcg ctaccgaagg gacgctttcc tggcggcaag
    15781 ccatggccca ggcggggaag ggagagcgtt acgaccagat actcgcgttt gcttatcccg
    15841 acaacagcct tagccgctgg ggagcgccac gcacaacctg tcagttatta cccaaagcga
    15901 aagcctggct ggcgaaaaaa atgccgcagt ggcggcgtat attacaaggc gagacggggt
    15961 acaacgaacc agacgtgttt gcggtctgtc gtttagtctc aggtttcccc tataccgatc
    16021 ggcagcagaa acggctgttt attcgtaact tctttacgct tcaggatcgg ctggatttaa
    16081 cccatgagta tctgcacctg gcctttgatg gttatcccac cgggcttgat gagaactata
    16141 tcgaaacgct gacccgacaa ttattaatgg actaatgcta tgcgaaagat ttttcttccg
    16201 ttgttactgg tggcgctttc gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt
    16261 gacgcaccgc tctctggctg gcatccggca gagggcgaag atgccagttt ttcacaatcg
    16321 atcaattacc cggcgtcgtc cgtcaacatg gcagatgatc aaaatatctc agcgcagatc
    16381 cgcggcaaaa taaagaatta tgctgcggcg ggtaaagttc agcagggccg gctggtggtc
    16441 aacggtgcca gtatgccgca gagaattgaa tccgatggtt catttgcacg cccttatatt
    16501 ttcactgaag gcagcaacag cgtgcaggtc atcagcccgg atgggcaaag ccgacaaaaa
    16561 atgcagtttt actcaacgcc gggtacagga acgattcgtg cacgtttacg gctggttctc
    16621 tcgtgggata cggacaatac cgacctcgat cttcacgttg tcacgcccga tggcgaacac
    16681 gcctggtacg gtaacaccgt gctgaaaaac agtggtgcat tggatatgga tgtcacgacg
    16741 gggtacggac ccgagatttt cgccatgcca gcaccagttc acgggcgcta tcaggtgtat
    16801 atcaactact atggtgggcg cagcgaaacg gaattaacca ccgcccaact gacgctgatc
    16861 accgatgaag gatcggtcaa tgagaaacag gaaacattta ttgtaccgat gcgtaatgct
    16921 ggcgagctga cgctggtgaa aagttttgac tggtgatcca tcccttcaca caacctggct
    16981 ctttcttttt tcagataaga ctgctgggcg atctgccgac gccaggatat aaccattaac
    17041 aagcgtcggc agattggtca tttcttattt ttcgtactga gaactgtcca tttactccgg
    17101 ctttcctggt taaggcaatg gtttctaagc gatacgcatt attgcaatgg tgacagaaca
    17161 ttgcggtaac acgcttttac cgctacctta accacactcc atcggtcacc tgaggcggag
    17221 cttcgcccct ttgaaatacc ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat
    17281 gttcagagca ttggcgttag tgctctggct gttgattgct ttttcatccg ttttttacat
    17341 cgttaatgcg ttacatcagc gagaatcgga aattcgtcag gaatttaatc tgagttccga
    17401 tcaggctcag cgctttattc aacgcacctc tgatgtgatg aaagagctga agtacatcgc
    17461 cgaaaatcgc ttatcggcag aaaacggtgt gctttccccg cgtggacgag aaacgcaggc
    17521 ggatgtgcct gcgtttgaac cgctgtttgc cgactccgat tgttcagcaa tgagtaacac
    17581 ctggcgaggt tctctggagt cattggcgtg gtttatgcgc tactggcgcg ataatttttc
    17641 tgcggcttac gatctcaacc gggtattttt aatcggcagc gataacctct gcatggccaa
    17701 tttcggtctg cgtgatatgc cagtggaacg cgataccgcg ttgaaagctt tgcatgaacg
    17761 catcaataaa tatcgaaatg caccacaaga tgatagcggc agtaacctct actggatcag
    17821 cgaaggtccg cgccctggcg tcgggtattt ttacgcgttg acgccagttt atctggcgaa
    17881 ccggttgcag gcgcttttgg gtgtcgagca gaccatccgg atggagaact ttttcttacc
    17941 gggtacgttg ccgatggggg ttaccattct tgatgaaaat ggtcataccc tgatttcgct
    18001 taccggacca gaaagcaaaa ttaaggccga tcctcgctgg atgcaggaac gctcctggtt
    18061 tggctatacg gaagggttcc gggagctggt gctgaagaaa aatctgccac cctcatcgct
    18121 cagcatcgtg tattcggtgc cggttgataa ggtgctggaa cgcattcgca tgttgatcct
    18181 taacgcaatt ttgctgaatg tgcttgccgg agctgcattg tttactctcg cgcggatgta
    18241 cgagcgacgt attttcattc cggcggaaag cgacgccctg cgactggaag aacatgagca
    18301 gttcaatcgc aagattgtcg cctccgcgcc agtgggtatc tgcattttgc gtaccgctga
    18361 tggcgtcaat attttaagta acgaactggc gcatacctat ctcaatatgc ttacgcatga
    18421 ggaccgccaa cgactgacac aaattatctg tgggcagcag gtcaattttg ttgatgtcct
    18481 gaccagcaac aataccaatc tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa
    18541 cgtggccatt tgtgtgctgg tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca
    18601 ggagatggca caagcagcgg aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt
    18661 cagtcatgag ctgcgaacgc cgctgtatgg cattatcggt aacctggatc tgttgcaaac
    18721 caaagagtta ccgaaaggcg tcgatcgtct ggtgacggca atgaacaact cttccagcct
    18781 gttgttgaaa attatcagcg atattctcga tttctcgaag attgaatcgg aacagttgat
    18841 gatcgaaccg cgtgagtttt caccgcgtga agtgatgaac cacatcaccg ccaactattt
    18901 accgctggtg gtacgcaagc agttaggctt gtactgcttt attgaaccgg atgtgccagt
    18961 ggccttaaat ggcgacccga tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc
    19021 cataaaattc accgataccg gctgtatagt tttgcatgtc cgcgctgatg gcgattatct
    19081 ctctatccgt gttcgcgata ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt
    19141 tgatcccttc ttccaggtcg gaacgggcgt acagcgaaac ttccagggga ccggtctggg
    19201 tctggcgatt tgtgaaaaac tgatcagcat gatggacggc gatatctcgg tagattcaga
    19261 accgggaatg ggcagccagt ttaccgtgcg tattccgttg tacggcgctc agtacccgca
    19321 gaaaaaaggc gtggaagggt tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc
    19381 gctctgtcag ttcctggaaa ccagtttgca gcgcagcggc atcgtcgtaa caacatacga
    19441 agggcaggaa ccgactcccg aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg
    19501 gcagggcaga gcggtagtga ccttctgtcg tcgacatatt ggtattccgc tggagaaagc
    19561 gccaggggag tgggtacaca gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg
    19621 tatttatttg atcgagatgg agagcgacga tcctgctaac gctctgccgt cgacggacaa
    19681 agcggtcagc gataatgacg atatgatgat tctggtcgtg gatgatcatc cgattaaccg
    19741 gcgtttgctg gcagatcagt tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg
    19801 cgtcgatgcg cttaatgtac ttagcaagaa tcatattgat atcgtgctta gcgacgtcaa
    19861 catgccaaat atggatggtt accgcttgac acaacgcatt cgtcagttgg gactgacgtt
    19921 gccggtaatc ggagtaacgg ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc
    19981 cggtatggac agctgcctgt cgaagccagt aacgctggat gttataaaac agacgctgac
    20041 ggtatatgcc gagagggtca ggaaatcgcg ggaatcgtag gacggataag gcgtttacgc
    20101 cgcatctggc attcagtgca aatgccagat gcgacgctga cgcgtcttat ctggcctact
    20161 ttaatgtaat ctgttgaaat aatgggaatc gtaggacgga taaggcgttt acgccgcatc
    20221 cggcaatcgg tgcaaatgcc agataagaca ctaacgcgtc ttatctggcc tacagatgat
    20281 tagtctttat ctgccggact taacgttact gaagagagat aattcagcag ggcgatatcg
    20341 ttctcgacac ccagcttcat catcgcagat ttcttctggc tactgatggt tttaatactg
    20401 cggttcagct ttttagcgat ctcggtcacc aggaagcctt ccgcaaacag gcgcagaact
    20461 tcactctctt ttggcgagag acgtttgtca ccgtaacctc cagcactgat tttttccaac
    20521 aggcgagaaa cgctttccgg ggtaaatttc tttccttttt gcagcgctgc gagggctttc
    20581 ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt cgatatccag atccaatacc
    20641 gcactaagaa tcgccgggtt gttgttcatg gtcagaacaa taatcgacag gcttgggaaa
    20701 tggcgcttga tgtacttaat taaggtaatg ccatcgccgt acttatcgcc aggcatggag
    20761 agatcggtaa tcaacacatg cgcatccagt ttcggcaggt tgttgatcag tgctgtagag
    20821 tcttcaaatt cgccgacaac attcacccac tcaatttgct caagtgattt gcgaataccg
    20881 aacaagacta tcggatggtc atcggcaata attacgttca tattgttcat gtaataggct
    20941 accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt tttctattcc
    21001 tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct tgccggatac
    21061 cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta acgcagcaaa
    21121 gtcactggtt gctgcttcag tatacagcct cttaacatca tccggtactg tgtctacaaa
    21181 gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca gaggcgattc
    21241 tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt cctgcatagc
    21301 gttgctcata ttgaagttga cgcacaattg accaggccca atttcccgta cgccagactc
    21361 atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg ttaaaaagat
    21421 atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac cccaattttc
    21481 taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca ttacgcagac
    21541 atcatccagt aaacgctctt cttcctcttc aacttccggg tcagctgcga gcattttgat
    21601 atgcacagag tagcgtgtac caagcccatc ccgcgttttg atgtttaaat gaccgcccag
    21661 tttacgtgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc catagcgatc
    21721 gttttgggtc tggttgataa acgggaagtg caaattatcc atttcatgaa tgcttacgcc
    21781 ttctccggtg tccagaatgc ggaacgtcag gcggtcttcg gaggactcat cctgatcaac
    21841 ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca gtagcagcaa
    21901 aatacgtcgt aatgcatcgc gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt
    21961 aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca caacttcatc
    22021 aattaaatcc tgcacggaga acagcaccgt ctcacttttc cagctatcgt ccgcaagcat
    22081 gttcgctaac tgtatttcat cgaccaatcg caccagcaca tctgcctgat tcgccagttg
    22141 tttgctttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg cgggttcttt
    22201 cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt tctcatacag
    22261 acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat cgcgaataat
    22321 gaaaatttgt gtgcgcggcg caacctggct gcggaacatg cggatctcat acagctcgtt
    22381 attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga tgttttgcag
    22441 attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgacagtgc ggttcgattc
    22501 ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat tgattgcccg
    22561 taaaatgcgc aattcgttat tgaccgccgt gttgggtaga ctttctgtac tgcgactgga
    22621 gaaatggcgg aatgtggtat agccaaataa cgccagcgcc agcaaaccga tgttcagcag
    22681 cagtggcagc agaatgtttt gcaacgtatc cagcaataag gtgccataag gaacctgcca
    22741 gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg tactgttaaa
    22801 gtggatactg acgctatccg ttccttcttt ctcattatca ttgtttcccg ttgccgtcgc
    22861 gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat cattaatcgg
    22921 cagatcaaaa gccacgaccg ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt
    22981 aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc
    23041 gagggcgttg gcctgttgca acatctctgc acgacgtgaa tcaacaatgt cactgacggt
    23101 cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca gcaccagact
    23161 gttatcctga ccattcaggt aatacattga ccacggtaca ttttctgcgc cccacaatgt
    23221 gtccagatag gtggacatcc gttgagtcat ctcaagcgtt gagttgtcgt gagagccaaa
    23281 gattaacgct tccgttttgc ggcgcggttt ttccagatag tagacatcct gtttcaggcg
    23341 cgtctcttgt aaaccttcgc cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat
    23401 ctgccaggtc acgtaacgcc aggtatcgac gcgcttttgg atagcgtggg taatgtcgac
    23461 aatttggtaa cttttatctt tcagccaggc gttaacggcg ctttgtacca ttacacccat
    23521 cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag
    23581 tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa
    23641 acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg
    23701 attgatgatt ctctattctc ccgtgatatt tatcatcctg cattggggga aatagatgta
    23761 caagcgccat tttgctgtac aaataaccta caaaaagccc aacgaaaata attgcaaata
    23821 aacaaagatt gctggaaatt atgctgatgt taattatttg tgaaatagtt aacaagcgtt
    23881 atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt aaaaaaaacc
    23941 ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa
    24001 taaataaagt taatgatgat agcggtagtt attctagttg cgagtgaagg ttttgttttg
    24061 acattcagtg ctgtcaaata cttaagaata agttattgat tttaaccttg aa
//
LOCUS       DAZGXS010000069        23268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGXS010000069 DAZGXS010000000
VERSION     DAZGXS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1106
                     /locus_tag="ACHKG5_002803"
     CDS             <1..1106
                     /locus_tag="ACHKG5_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0868873.1"
                     /translation="SLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQL
                     SFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVV
                     DYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLL
                     LTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYF
                     FNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDI
                     LARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFST
                     GDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1287..2777
                     /gene="dgcJ"
                     /locus_tag="ACHKG5_002804"
     CDS             1287..2777
                     /gene="dgcJ"
                     /locus_tag="ACHKG5_002804"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0868874.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2820..3323
                     /gene="yeaK"
                     /locus_tag="ACHKG5_002805"
     CDS             2820..3323
                     /gene="yeaK"
                     /locus_tag="ACHKG5_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0868875.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3324..3428)
                     /gene="yoaI"
                     /locus_tag="ACHKG5_002806"
     CDS             complement(3324..3428)
                     /gene="yoaI"
                     /locus_tag="ACHKG5_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0868876.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3598..4044
                     /gene="yeaL"
                     /locus_tag="ACHKG5_002807"
     CDS             3598..4044
                     /gene="yeaL"
                     /locus_tag="ACHKG5_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0868877.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4001..4822)
                     /gene="nimR"
                     /locus_tag="ACHKG5_002808"
     CDS             complement(4001..4822)
                     /gene="nimR"
                     /locus_tag="ACHKG5_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0868878.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4919..6100
                     /gene="nimT"
                     /locus_tag="ACHKG5_002809"
     CDS             4919..6100
                     /gene="nimT"
                     /locus_tag="ACHKG5_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0868879.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6155..6502
                     /gene="yeaO"
                     /locus_tag="ACHKG5_002810"
     CDS             6155..6502
                     /gene="yeaO"
                     /locus_tag="ACHKG5_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0868880.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6524..6778)
                     /gene="yoaF"
                     /locus_tag="ACHKG5_002811"
     CDS             complement(6524..6778)
                     /gene="yoaF"
                     /locus_tag="ACHKG5_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0868881.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            6961..7986
                     /gene="dgcP"
                     /locus_tag="ACHKG5_002812"
     CDS             6961..7986
                     /gene="dgcP"
                     /locus_tag="ACHKG5_002812"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0868882.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8019..8117
                     /gene="yoaK"
                     /locus_tag="ACHKG5_002813"
     CDS             8019..8117
                     /gene="yoaK"
                     /locus_tag="ACHKG5_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0868883.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8120..8194
                     /gene="yoaJ"
                     /locus_tag="ACHKG5_002814"
     CDS             8120..8194
                     /gene="yoaJ"
                     /locus_tag="ACHKG5_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0868884.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8253..8501)
                     /gene="yeaQ"
                     /locus_tag="ACHKG5_002815"
     CDS             complement(8253..8501)
                     /gene="yeaQ"
                     /locus_tag="ACHKG5_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0868885.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8648..8830)
                     /gene="yoaG"
                     /locus_tag="ACHKG5_002816"
     CDS             complement(8648..8830)
                     /gene="yoaG"
                     /locus_tag="ACHKG5_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0868886.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8834..9193)
                     /gene="yeaR"
                     /locus_tag="ACHKG5_002817"
     CDS             complement(8834..9193)
                     /gene="yeaR"
                     /locus_tag="ACHKG5_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0868887.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9366..10004)
                     /gene="leuE"
                     /locus_tag="ACHKG5_002818"
     CDS             complement(9366..10004)
                     /gene="leuE"
                     /locus_tag="ACHKG5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0868888.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10131..11054)
                     /gene="dmlR"
                     /locus_tag="ACHKG5_002819"
     CDS             complement(10131..11054)
                     /gene="dmlR"
                     /locus_tag="ACHKG5_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0868889.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11157..12242
                     /gene="dmlA"
                     /locus_tag="ACHKG5_002820"
     CDS             11157..12242
                     /gene="dmlA"
                     /locus_tag="ACHKG5_002820"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0868890.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12493..14103
                     /gene="yeaV"
                     /locus_tag="ACHKG5_002821"
     CDS             12493..14103
                     /gene="yeaV"
                     /locus_tag="ACHKG5_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0868891.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14135..15259
                     /gene="yeaW"
                     /locus_tag="ACHKG5_002822"
     CDS             14135..15259
                     /gene="yeaW"
                     /locus_tag="ACHKG5_002822"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0868892.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15315..16280
                     /gene="yeaX"
                     /locus_tag="ACHKG5_002823"
     CDS             15315..16280
                     /gene="yeaX"
                     /locus_tag="ACHKG5_002823"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0868893.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNLYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16334..17449)
                     /gene="rnd"
                     /locus_tag="ACHKG5_002824"
     CDS             complement(16334..17449)
                     /gene="rnd"
                     /locus_tag="ACHKG5_002824"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0868894.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17531..19216)
                     /gene="fadD"
                     /locus_tag="ACHKG5_002825"
     CDS             complement(17531..19216)
                     /gene="fadD"
                     /locus_tag="ACHKG5_002825"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0868895.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19421..20002)
                     /gene="yeaY"
                     /locus_tag="ACHKG5_002826"
     CDS             complement(19421..20002)
                     /gene="yeaY"
                     /locus_tag="ACHKG5_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0868896.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20042..20737)
                     /gene="tsaB"
                     /locus_tag="ACHKG5_002827"
     CDS             complement(20042..20737)
                     /gene="tsaB"
                     /locus_tag="ACHKG5_002827"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0868897.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20795..22705)
                     /gene="yoaA"
                     /locus_tag="ACHKG5_002828"
     CDS             complement(20795..22705)
                     /gene="yoaA"
                     /locus_tag="ACHKG5_002828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0868898.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22837..23181
                     /gene="yoaB"
                     /locus_tag="ACHKG5_002829"
     CDS             22837..23181
                     /gene="yoaB"
                     /locus_tag="ACHKG5_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0868899.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5698 a         5935 c         5664 g         5971 t
ORIGIN      
        1 taagccttgc atttttaagt ggccttatct atttagttga aaccattgta attatccata
       61 aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata
      121 ttttccgcca actcagtttt atttgtttaa cctcgctggc gctcttttgt tatggaaaag
      181 acaacatcct tgacaacaat aagaaaaaaa ctggcattct gttgctggcg ctgatccctt
      241 ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt
      301 atgtcgtcga ttactgtccg gacaatcata ctgcgacctg gggaatcaac tatacaaaaa
      361 tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
      421 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
      481 tactgactct ggatgagtat aattatacca tctggtatat cagtcgcggg attgaagtct
      541 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggag ctgcaactct
      601 ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca
      661 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttctgtgtc atgctggttg
      721 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
      781 tggtttcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac
      841 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
      901 ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
      961 ttcctgaaca aatgaccatc agtattggca cagttttttc aacgggtgac acgcgtaata
     1021 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
     1081 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
     1141 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     1201 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     1261 cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta
     1321 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg
     1381 acagagcaat gagtgcctat ctgcgctata tcgtgcagaa agcagactcc tcctttcttt
     1441 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
     1501 agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata
     1561 atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc acattacaaa
     1621 ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc
     1681 aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
     1741 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta
     1801 atgttgaata ctttgcgatg aataactggt ccatcctgca gtcaggaagt attggcatcg
     1861 atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc atttaccagg
     1921 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtgtatgtt gcagggcagc
     1981 taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc ttttacactc
     2041 atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgataca gattcggggc
     2101 gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg
     2161 acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt
     2221 cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga
     2281 tgcacttccg cttataccaa aatgtgtcgc gagaaaatat tagtgatgcg atgactggac
     2341 tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa ctggtgcaat
     2401 ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcta ataaatgaca
     2461 ccctcggtca tcaggaaggg gatttagcga ttacgttgtt agctcaggcg attaaacaat
     2521 cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg
     2581 tcgattcgac gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac
     2641 atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg
     2701 atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca
     2761 aaaacagccg ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa
     2821 tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag
     2881 aaggtgctga tttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag
     2941 aaattcgtgg caccgctcta ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca
     3001 atggcgtaaa tcaacatgtt ctggcgatcc tcgcagccga tcaacaggcc gatctgagcc
     3061 aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg gaagtcgatg
     3121 aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac
     3181 tggttgccga ccctctactc ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc
     3241 tggataaatc cgttattttg aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg
     3301 tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac ggacaaaacc
     3361 agcacaacag caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga
     3421 tcgttcatag catacccccg ggagatagct gttttcacgt tattgttagc gtgcacaaat
     3481 ggcagtttga tgacagttcg cctgattttt tatgcaaaaa acgtaaagat tttctacttc
     3541 cttcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga aaccacgatg
     3601 tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac
     3661 accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc gttaagcacc
     3721 tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc
     3781 gttatggccc ccatcgccag cgggacgcta ccgccctcaa ccttgattca ctcgtttctg
     3841 aactggaaat cgctggtggc aattgcagta ggcgttattg tctcctggct gggtgggcgc
     3901 ggcgtgacgt taatgggcag ccagccgcaa ctggttgctg ggctgctggt gggcactgtt
     3961 ttaggcgtag cgctgtttcg cggcgtaccg gttggcccgc ttattgccac gggtctggtt
     4021 tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca
     4081 ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa
     4141 ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc tgtaactgct
     4201 gtcgccactg gcgaaaactc aaccccgtct cttttactat cagtcgggca agattgcgtt
     4261 cactcatcgc aaaaaagcca gcccattgcc ccaatgcccc ccactcgacg ggctctttcg
     4321 ccatcatctc caccatcgcg cggattttag gatgagaaga aacgggtagg tgcagctgtt
     4381 gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct
     4441 cagcacgctg agtcgtcgtc ctgtttgcca gagttaaaat taattcccgg cataagggcg
     4501 atattttcaa tgtgtaacat gttgtcggca ttgtcactgc gctgggttcg ataaacagaa
     4561 aacagagttc agcgttagct gtcacttgat tgctatgttc caccccgccc ggtatccaaa
     4621 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
     4681 gtagcgccag tatcagctgt cctttgcggt gctgatgtac aggactggtt aactcatccg
     4741 ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat
     4801 tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg
     4861 attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat
     4921 gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat
     4981 gattgccact acattacgcg tcaccttcac cggcgcagca ccgttactcg atacgattcg
     5041 ttccgcttac tcgctgacga ctgcgcaaac cggcttattg accaccctgc cattattggc
     5101 ctttgcgcta atctcacctc tggctgcccc ggtggctcga cgttttggta tggaacgtag
     5161 cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgctctc tcccttcgcc
     5221 ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag gtaatgtctt
     5281 actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata
     5341 ttccctgaca atgggagctg cggcggctct gggatcggct atggtagtgc cgctggcttt
     5401 gaacggtttt ggctggcaag gcgcgttgct catgctgatg tgttttcctc tgctggctct
     5461 ttttttatgg ctgccacagt ggcgaagcca acaacatgca aatttgagta cttcacgcgc
     5521 cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg
     5581 gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgatcc tcatcagtca
     5641 cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc
     5701 tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa gaccagcgtg gtattgcagc
     5761 gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgcttcatgc cagcgcacgc
     5821 gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac
     5881 gttcattggt ctgcgggcta gttctgcgca tcaggcggcg gcactctcgg ggatggcaca
     5941 atccgtcggg tatttgttgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc
     6001 taacggtgac tggtccgtac cactaatggg tgttgcaata ctttcactac tgatggcgat
     6061 tttcggactt tgtgccggga gagacaaaga aattcgctaa tatccggtac tatagtgacg
     6121 tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca aacgcgttta
     6181 tgatccggca gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccacgcgg
     6241 tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc cgtcaacgga
     6301 actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct
     6361 tgcagaactg gcacaacacg agcaagaagg aaagcggctg gcggacatcg ccaaaaaaca
     6421 gccgctgacc ctgctctact cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc
     6481 cgactggcta cgtagcttgt gattttagta cagcatccgg cggttatttt tcaccagccg
     6541 gatggtccag tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat
     6601 tgttgctgac cccttgcgct gtctgcactg gaatgagcgt cccgcccttc tgctggcaat
     6661 agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag
     6721 gggttgaaca accagccagg accagcaagc aaggcagaac aaaactgata attttcattt
     6781 attgatctca catatgtatc caagcttaga gtatcgcggt atcgttttgt tttgcagcac
     6841 tatttttatt acattcactc aaaacatatt acgtcttgtt tcatctttgt tgatgatgtt
     6901 ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc
     6961 gtgtcagatc agattatcgc ccgcatctcg caatcccttg ccaaagaaca gtcactggaa
     7021 agtctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac
     7081 ctgaccaaag tggatgtcga agcgcgcctg cagcatatta tgtttgcccg taacagccag
     7141 aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc
     7201 attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg
     7261 gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc ggatggatca
     7321 ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa
     7381 caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcactggtt
     7441 gaacagctgc gcgaagccaa tgctgcgctg attgcgcaat cgtataccga ctcgttaacc
     7501 gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc cctcgcccgg
     7561 catcttaacc aaaagataat gatcgcgttt atcgatctgg ataacttcaa attaatcaat
     7621 gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga gcgccttaat
     7681 acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt
     7741 tcactaaaca acgagaatgc ggatatttcg tcgctgcgag aacgcattca gcagcaaata
     7801 cgcggagaat atcacttagg tgatgttgat ttgtattatc ctggtgccag tcttggcata
     7861 gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tacatgctgc cgatatcgcg
     7921 atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcattc agcgccacat
     7981 tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc
     8041 cggttgtaat cttcattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg
     8101 ctgccccggg agcataaaga tgaaaaaaac aacgatcatt atgatgggtg tggcgattat
     8161 tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctaaaaaa tagcaaaggc
     8221 tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatcttcct gtagataaat
     8281 agcacgacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact
     8341 ttaccaaagc caaacagcgt gctgatccaa ccgccgacta cggcaccgac tatccccagc
     8401 aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata
     8461 ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt
     8521 tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga gggcaagatt
     8581 tgtgacgagc atcacggagg tttttttgcg atggcgcaga aattgcgcca tcaacgatca
     8641 gtgataatta ccaaccacaa acatcatgtt cgttttccgt gtcataagaa cgcacggtat
     8701 tcaccagatc ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct
     8761 ctgtttcata atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg
     8821 ctttgcccat gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca
     8881 ggacttcagg agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa
     8941 tgttgtgcca cttttctgga gggaacaccg caaactgccc cgcttcgata aggatcacct
     9001 gatcaggctc tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa
     9061 cggaaaggcg tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc
     9121 cggcaggcgc agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg
     9181 ggatttgcag catccttttc cctccttcgg tgaatgcgct gaaaacggtt tattccagcc
     9241 gtttcagggt acgcctgata atttgctttt taaataccat ttattggtta ctttttagca
     9301 ccatatcaga gaaaaatcag ggaggattat agatgggaag cccgcgacaa cgcgggctga
     9361 aagcatcagg attgcagcgt cgccagtcgg gcagcgaaac ccacgaacat caaaccaatc
     9421 agtgagttgc ccactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca
     9481 ccagatataa tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga
     9541 ataaagaatg aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac
     9601 acatagaaca aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaata
     9661 gcaccgtatt ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtaa
     9721 agaattttac tccccagata gagcaaataa aacgcaccaa gataacgtac aatgttgaat
     9781 aatatcgggg tggtcttaat taatgtcgcc attccagccc atgccagaaa catcaatacc
     9841 gcatcgccaa taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg
     9901 ctatttttga gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca
     9961 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
    10021 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
    10081 ggagaaaatc aggttttaac ctgatatcaa cccgataatt gaatcattaa tcaggcatgc
    10141 tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
    10201 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
    10261 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
    10321 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
    10381 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
    10441 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
    10501 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
    10561 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
    10621 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
    10681 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
    10741 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
    10801 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
    10861 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
    10921 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
    10981 acggcggcaa aaccagcccg gcgagccacc agcataaaga cgcgcaaatc attcagcagc
    11041 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
    11101 agtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
    11161 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg
    11221 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgccttg agttttgagc
    11281 aaatggagtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
    11341 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggaca
    11401 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
    11461 agtacgtcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
    11521 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
    11581 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
    11641 ttacgcgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
    11701 cacgtaagac gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact
    11761 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
    11821 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
    11881 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
    11941 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccctcgctc ttcgagcctg
    12001 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacaattt
    12061 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
    12121 ataacggtat tctggcagcg attgaagaag tgattgctca cgggccgaaa acaccggata
    12181 tgaaaggcag tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
    12241 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttgttatt cgttctttgc
    12301 agttaataac ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc
    12361 gtattcttaa ttgtttaatt tatgtaacat gcaaataatc aattcgttaa acacgacata
    12421 caactcatta ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc
    12481 aggagatgac tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc
    12541 ctggtggcca ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac
    12601 gcggccaacg caatatacac ttttgttacg cgtacgttag gttccgcagt acaggtattg
    12661 gttttgctgg caatgggact agtgatttat ttagccacca gtaaatacgg caatattcgt
    12721 cttggcgaag gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc
    12781 ggtttaggtt cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg
    12841 cctggattaa atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc
    12901 ttcttccact ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct
    12961 tatcactttc atgtgcggaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt
    13021 accggcgttc gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc
    13081 actgtcggcg cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt
    13141 tccgcactga ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc
    13201 ggcggcattt tttgcctaag ctcatggatt ggtatcaaca acggtttgca acgtctgagc
    13261 aaaatggttg gctggggcgc gttcctgctg ccattactgg tgctgattgt cggtccaacc
    13321 gaatttatta ccaacagcat catcaatgcc atcggcctga ccacgcaaaa cttcctgcaa
    13381 atgagcttat tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc
    13441 tactggctgt ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc
    13501 cgcggtcgta agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc
    13561 tggttcttct tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc
    13621 aacgtcccac aggtgctgga aactctgggc ggcgaaacag ctgtacagca agttctgatg
    13681 tcgttgccag ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt
    13741 gcctcgcata tggatgcagt ggcctacacc atggcggcga ccagtacgcg taatctccag
    13801 gaaggtgacg atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc
    13861 ccgctttcca tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc
    13921 acagcccttc ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg
    13981 aaacaggatt acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg
    14041 gttgaagccc tggaaaaaac accagtgctc cctgcgggaa ccgtattcaa aggcgacaac
    14101 tgagcgccat catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt
    14161 actacccgaa aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta
    14221 taccgatcag aacgcctttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg
    14281 cgtcgctcac agcagcgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg
    14341 cgaaagcatc gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg
    14401 tccgcaccgt ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg
    14461 cccatatcac gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga
    14521 aaacgtcgcc aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata
    14581 tgccggattc gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc
    14641 cggtctgggc gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc
    14701 ccgctttacc acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg
    14761 ctatcactgt ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg
    14821 gcacaccatg cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc
    14881 gtttaaattt gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac
    14941 gatgctgaat gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga
    15001 ttctgaaact accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga
    15061 gcaaaaatcg ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt
    15121 tgaaagcgta cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc
    15181 cgacagtagc ggtagcggca tttccgaaca tggtatcgcc catttccata atctgctggc
    15241 gcaggtattt aaggactaat gacatcggcg gcggtatttt ccgccgccgg gctgattttt
    15301 gatggagtac agcaatgtca gactatcaaa tgtttgaagt acaggtgagt caggtggaac
    15361 cgcttaccga acaggtgaaa cgcttcacgc cggtggcaac cgatggcaaa ccgctgcctg
    15421 cgttcaccgg aggaagtcac atcatagtgc agatgagcga tggtgataac ctgtacagta
    15481 atgcgtattc actactgagt tcaccgcata acacctctag ttatcagatt gccgttcgac
    15541 tggaagaaaa ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc
    15601 ggttaacgat ctcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc
    15661 tgtttatcgc gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagttgcaac
    15721 acagcgacgt cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc
    15781 gtgatgagct agtccagcat ccgcaggctg agaaagtcca tttgcatcat tcatcaaccg
    15841 gaacacgact ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata
    15901 cctgtggccc cgaggcgcta attgaagcgg taagaagtga agctgcgcgt ctggacatcg
    15961 ctgccgatac gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgcattta
    16021 ccctggtgct tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc
    16081 aggttattga aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg
    16141 gaacctgcga aacggcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg
    16201 atgaagagcg tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac
    16261 gcctggtgtt ggatttgtag tttgccgaag ccggatgtgg cgctgagcgc gcccagtccg
    16321 gcttcggaaa attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca
    16381 gctcaccacg ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt
    16441 gccagttcag cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttgt
    16501 gcgtttcgct cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataacccg
    16561 gcatgtccat caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt
    16621 tttccaccag cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca
    16681 ggctgtccag ttcgcctaaa cttcccggca tataacgcgc taccgaccac aaatgctctt
    16741 cacgcacgac aaagttcacc gccagatcgc gctctcgcgc cttgcgcagt cgccagtcgg
    16801 ctaacagttg cagacaggcc agttggcgtg tgcgtaattg ccaggcattg gtgatatcac
    16861 gccaggcatc ttccggcgca acgacttcct gacgacgcat ttgcatcagg cgacattcat
    16921 ccagcgccgc tggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg
    16981 gtaacagata ccagacatcc gccgctgcat attcacactg acgttcggtc agcggtctgg
    17041 ccagccagtc ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca
    17101 tggaagcgaa accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa
    17161 tcaggggttg tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg
    17221 catggagaaa ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg
    17281 tgatcccgag aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca
    17341 gctgcgggta ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg
    17401 cacggacggc ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca
    17461 aattgttttc tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact
    17521 gacttaacgc tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc
    17581 gcaaaatttt tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta
    17641 ctttgtagcc cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg
    17701 gatctttttt cactacgaag attttcaccg cttcaccact ggagccggaa ggtacaccaa
    17761 cagccgcgac ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat
    17821 agacgttaaa accggaaacc aaaatcatgt cttttttacg atcgacaatg cgcaggaatc
    17881 cttcttcatc cattaccgcg atgtcgccag tgtgtaacca gccgtttttg atgatttcat
    17941 cggtagcatc cggacgctgc caatagccca gcatcacctg tggacctctg acgcaaagtt
    18001 cacctggctg acctggcgat acttcattat cgtcgtcatc caccagtttg gcttccgtcg
    18061 acggcaccgg caaaccaatg ctaccgctat gataatcaat atcatagggg ttcacgctga
    18121 ccagcggcgc acattcggtc aggccataac cttccagcag atactgtccg gtcagtttca
    18181 cccagcgttc tgccaccact tgctgaacag gcatcccacc gcctgcagaa aggtgcagac
    18241 tggagaaatc cagttgttga aactctttat tgttcagcaa cgcgttgaac aaggtgttta
    18301 cgccggtgat agcggtaaac ggatatttcg ccagctcttt gaccagcccc ggaatatccc
    18361 gcgggttagt gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg
    18421 tcagtgcaaa aatgtgatac agcggcagcg ccgtcaccac cagctctttg cccggatgca
    18481 gcagaggccc atacgtagcg ttaacttgtt ccaggttcgc cagcatattg cggtgagtca
    18541 gcatcgcgcc tttcgccaca cctgtggtgc cgccagtgta ttgcaggaag gctaaatctt
    18601 ctggcaccag ttcaggtttg acatactgca tacggtagcc gttgtgcagg gcgctacgaa
    18661 atgaaatggc atctggcaga tgatatttcg gcaccagacg tttgatgtat ttaacaacga
    18721 agttaaccag tgtgcctttc gctgtggaaa gctgatcgcc catacgggtc aggataacgt
    18781 gctgaactgc ggttttatcg accacttttt ccagcgtgtg agcaaagttg gacacaatga
    18841 caatcgccga tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg
    18901 ggttaacgtt tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt
    18961 gcaataaatt aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt
    19021 gcaaataagc ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc atcacctccc
    19081 ccatattcac aaacgcaggc tgatcggcgt agcgcgcgac cgactgctca aacatatcta
    19141 ccagagattg ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa
    19201 gccaaacctt cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa
    19261 atgatataca tgccgttaac ataatattaa ctcatcatac cagcttgata attacccaac
    19321 gaaaaggttg cgaagcgcgt cactatttat ttttatcttt accgtaagaa tgcagaaaca
    19381 gcggaccagc cgctgtttct ttttctttaa aaacaagcaa ttattcagtt acaactgttt
    19441 gtactctcgc ggggccggga ttataccagc cccatccgcc gtggccatag ggccagccac
    19501 gaccgccata aaaccaggga tcaatcggct gaggcggcat aatcacctgc tgggttaaat
    19561 gccaacgttt gtaacctgtt gcctgcatca ccataaattt atagggcgtg ttgccgattt
    19621 ttccgtcaac cgcaccagtg attggcccga ctaccgtaac cagttgtcca cggaaatcca
    19681 ccgggtccag aaaaccgttc acatcggcat aaatgcgacc gcgagaaggt tctcccagcg
    19741 tcggtctggc tccgctgtcc agcggtacgg tagcaatttc caggcgggtt ttcccttgct
    19801 ggttttgtac cgcaaccact ttgccaccaa agcgtgcctc ctgaccaacg tacagctgcg
    19861 gcgcactcat cacccgaact aaatcttgtt gcggcgtggg actgctgcct ttaatggcgt
    19921 ccggcacagt gacacaaccg ctcagcatta gcgcaaacgt acctgccagt atgcctttga
    19981 taacattctt ttgaaccgcc atgatgcgac tccttttttt ctcagggcat actcttaaga
    20041 ttcattcttt gcccggaagt ttcttccatg cgacgttgtt acgtaaataa accggttcgg
    20101 catgttccac cgccaccgtt ttaccctcgg caaacatctg acaagcaatc ggcagcatat
    20161 cttctgcagc gggcagtaaa acttctccgt cgcgcaaaac cagtccgctc tctttaccga
    20221 gatccggcca ggcttgccag cctgtaccta ccgtcaccca ttccccggaa agctgttgca
    20281 ttcgttcatg aacgagttcg ggtttgagta cggcttcggt ttcttcaccg tgccagatac
    20341 cgttttcatc acgctgatat tcggcccagt aaacttcgcc cattcgcgcg tcaatggctg
    20401 acagcacgcg tgttgcgccg tttttgcgcc acgccccttg cgccatcgtc attagcgtgg
    20461 agacgccaat catcggtaat tccgcgccaa gcgccagccc ttgcgcgatg ccaataccaa
    20521 tgcgcacgcc tgtaaagcta ccggggccgc gcccgtaagc cagagcgtta atatcagtca
    20581 gggaagttcc gctggtggtc aggatatcct gcaccatcgg taagattcgt tgagtatgtt
    20641 cacgagggca aagctcaaaa tgagcgttga cagtaccgtc gttccacagg gcgacagagc
    20701 aggcctctgt cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct tagatcaata
    20761 aaaaggcgcg catcatacca tactccgtaa caaattacct ggaggatggt atcgcaagga
    20821 aacgaaccgc acgggcaatg tcacgggtgc gtggcgcggg cggcagactg gcgagaaacg
    20881 tcgcgccgta aggacgcatc accagccgat tgtcacaaat taccaaaacc ccgcgatcgt
    20941 cggcgtcgcg aatcagtcgc ccgactccct gcttgagagt aatcaccgca tccggcagtt
    21001 gtacttcatc gaacgggtcg ccaccgcgca aacggcaatc ttccatgcgc gcttttaaca
    21061 atggatcatc aggcgaggta aacggcaatt tgtcgataat caccaatgac aatgtatcgc
    21121 cacgcacgtc caccccctcc cagaaactac tggttgccac aagaagcgcg ttaccggcac
    21181 tgacaaattg ctgcaacagt tgccctttgc tggtttcccc ctgcaataat acgggaagcg
    21241 tcatggtagc gcggaactgc tcggccagat cgcgcatcat ggcgtgcgag gtacaaagca
    21301 taaaacagcg accgttgtta gcttcgatga tcgggcgcag catagccgcc agttgccgcg
    21361 cggaacccgg ttgattggtt tgcggcaggt tgcgcggcac acagagtaac gcctggcggc
    21421 tgtaatcaaa cgggcttggc aatagcaacg actccgcctg ttctatccca agccgcgagg
    21481 tgaaatgatg cagatcgtcg tttaccgaca gcgttgctga ggtgaagatc cagctaccgg
    21541 gcttttgcgc cattaactct ttgaatttat ccgccacgct gagcggcgtg agagccagag
    21601 taaaatggcg cgaagtgcat tcgtaccagt agctgtagcc cggctgattg atctctttta
    21661 gccgcttcag ccgtgtgcga tacaacgtgg cgcgctcaaa tgccgcatcc agcaaggcgg
    21721 aacgccccag tgacagtttc gccacgtcat aacaaagttc cagggtgtca tcgagcagta
    21781 aaaatgcccg ctgaatttgc ggattagcta acagctcacg caggttaccg cgataacctg
    21841 gttcaccgag ttgcagacga aaatcctgcg cactctgggc aagacgatca gcgcactttt
    21901 gtaactgctg ggtgtctttt aattcggtgc ggtaggcgat ggtgatgtct tttgccaggt
    21961 cgagcagttg tcgactggag agtgactgac caaaatactg gctggcaatg tccggtagct
    22021 ggtgggcttc gtcgaagatc atgacgtccg cttccgggat caactcgcca aatccactct
    22081 ctttaaccac catatccgcc agaaagagat gatggtttac caccaccaca tcggcgtcca
    22141 tcgctttttt acgtgctttg accacaaagc aatctttata catcgggcag tcgctgccaa
    22201 gacagttgtc gttggtgctg gtgaccagcg gccacgcctg cgaatcttcc gccacgctga
    22261 cgcaggtgct gatatcacca tcgactgttt gattagacca ggagcgcagc aggatcacat
    22321 cgcttaatat ttgtaccgga agatcgcccc ccgccagcgc ctgctgttcg agacgttcga
    22381 ggcagaggta gtttgagcgc cctttcagca acgccacgtt gcccgtgtat ttcaacgcct
    22441 ttgagaccgt cggcaaatcg cggctataga gctgatcctg caacgctttt gagccggtcg
    22501 agataatgac tttctttttc gcccgcagcg caggggccag gtaagcgtag gttttgcccg
    22561 taccggttcc tgcttccacc accagcggct ggcctttttc tatcgcctgg gtgacggcta
    22621 ccgccatctg tcgctgtggt tctcgcggct taaagcctgg tatcgctttc gccagctgac
    22681 cgtctggtgc aaaatcgtcc gtcacactac cccctgttga tttgaacagg gattatgtca
    22741 ggatgagggc gctttcgcca gttgaagtgg tgacggcgac ctcacattgt ggcagtcttt
    22801 gcagcacgaa aatggaagga taatgaggat aaaatgatga ctatcgttcg tatcgatgct
    22861 gaagcccgct ggtctgatgt agtaatccac aacaacacgc tctactacac tggtgtaccg
    22921 gaaaacctcg acgccgatgc ctttgagcaa accgccaaca ccctggcgca gattgacgcc
    22981 gtgctggaaa aacagggcag caataaatcg agcattctgg atgccaccat tttcctggcc
    23041 gataaaaacg acttcgcggc gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat
    23101 gcgccggtgc gctgcacggt acaagcgggt ttgatgaacc cgaagtataa agttgaaatt
    23161 aagattgtgg ctgcggtgta agctttatcg aagcaaaata agtcagacga taatttatcg
    23221 ataatactgg tcggttatac ataaatcgac cagagaataa gatattac
//
LOCUS       DAZGXS010000070        22852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGXS010000070 DAZGXS010000000
VERSION     DAZGXS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            702..4514
                     /locus_tag="ACHKG5_002830"
     CDS             702..4514
                     /locus_tag="ACHKG5_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFP0868900.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4603..6222
                     /locus_tag="ACHKG5_002831"
     CDS             4603..6222
                     /locus_tag="ACHKG5_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP0868901.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            6238..6606
                     /gene="acpS"
                     /locus_tag="ACHKG5_002832"
     CDS             6238..6606
                     /gene="acpS"
                     /locus_tag="ACHKG5_002832"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0868902.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6620..7381
                     /locus_tag="ACHKG5_002833"
     CDS             6620..7381
                     /locus_tag="ACHKG5_002833"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP0868903.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7385..7933
                     /locus_tag="ACHKG5_002834"
     CDS             7385..7933
                     /locus_tag="ACHKG5_002834"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFP0868904.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7955..8236
                     /locus_tag="ACHKG5_002835"
     CDS             7955..8236
                     /locus_tag="ACHKG5_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0868905.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8272..9432
                     /locus_tag="ACHKG5_002836"
     CDS             8272..9432
                     /locus_tag="ACHKG5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFP0868906.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9506..12064
                     /locus_tag="ACHKG5_002837"
     CDS             9506..12064
                     /locus_tag="ACHKG5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFP0868907.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12069..13292
                     /locus_tag="ACHKG5_002838"
     CDS             12069..13292
                     /locus_tag="ACHKG5_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFP0868908.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            13289..14242
                     /locus_tag="ACHKG5_002839"
     CDS             13289..14242
                     /locus_tag="ACHKG5_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0868909.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTAIADKQFGYHYFDLEYLEG"
     gene            14253..14960
                     /locus_tag="ACHKG5_002840"
     CDS             14253..14960
                     /locus_tag="ACHKG5_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0868910.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14944..15600
                     /locus_tag="ACHKG5_002841"
     CDS             14944..15600
                     /locus_tag="ACHKG5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868911.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15601..16911
                     /locus_tag="ACHKG5_002842"
     CDS             15601..16911
                     /locus_tag="ACHKG5_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0868912.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16920..17708
                     /locus_tag="ACHKG5_002843"
     CDS             16920..17708
                     /locus_tag="ACHKG5_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFP0868913.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17705..19093
                     /locus_tag="ACHKG5_002844"
     CDS             17705..19093
                     /locus_tag="ACHKG5_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0868914.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19107..20048
                     /locus_tag="ACHKG5_002845"
     CDS             19107..20048
                     /locus_tag="ACHKG5_002845"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0868915.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20045..21031
                     /gene="ycdU"
                     /locus_tag="ACHKG5_002846"
     CDS             20045..21031
                     /gene="ycdU"
                     /locus_tag="ACHKG5_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP0868916.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21398..22600
                     /locus_tag="ACHKG5_002847"
     CDS             21398..22600
                     /locus_tag="ACHKG5_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0868917.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5971 a         5040 c         5954 g         5887 t
ORIGIN      
        1 gatgtatagc ggtgattgct attagatggg aatgaaagct tgccaaaccg ttttgctctt
       61 ctttctccct ttcaacgata agccaggcat aatgtggctg cttaagccaa ttgtcgcaaa
      121 gccaaagctc tctatatagc taacctcatg gttaatccga tctcagtaat accatgagca
      181 agaactcttt cctttttata taactcttaa gggttacatg cgtgaatgca aatggtaata
      241 taatgaaatg tcgcctgccc gtaatgacca taaagaatct accccgacgt aaaagatata
      301 gagaataaga tgcagtaatg atgatgcttt gttaatcaaa taataagatt taaattaatt
      361 gtaactcacc aactttgttg ttttattcgg atgtctgaaa tgatgtcgta cagaaaacta
      421 catatcattt cagacgtcct ctaaattgga tttttttcac ccaaggagca aggatggggg
      481 acttttaaat tattgtgttt tagtaagtta ggttcttcgc cgttcgatga aaaaacaata
      541 actcttagtt gtttttttga gtccttattt cagcttattt ttcgtgattt tttattgcgc
      601 caggtgatag gatttgccgc aatcattttt gctgatattc cgtgagtcat caaaagtgat
      661 ctgcatgtcc aaaatgaata gcatcaataa ggaaaacgtt agtggcgatg ataaatttaa
      721 gtaaggaagc aacggtgggg aaagcattaa cccctattgc tatacttatg atgttgtctt
      781 ttcctgtagc ttctcaagcg gcgggattag tcataaaaaa tggaacggta tataacgcca
      841 atggtgtgcc agtcgttgac atcaacaaac ctaacggtag cggtttatct cataatatct
      901 gggataacct aaacgttgat aaaaatggtg tcgttttcaa taatagcgct aatgaatcca
      961 gtacttcact tgccggaaat attcagggaa acagtaatct gacctccggg tcggcgaagg
     1021 tgatcctgaa tgaggttact tccaaaaatc cttcaaccat taatgggatg atggaagttg
     1081 caggggataa agcggatctg attattgcca acccgaatgg tattactgta aacggtggcg
     1141 gttcaatcaa tacaggtaaa cttaccttaa ccaccgggac gccggatatc caggatgaca
     1201 agctggccgg ttactccgtg aacggcggta ccattacgct cggtaaactg gataacgcca
     1261 gcccgacaga aattctgtcc cgtaacgtgg tagttaacgg caaagtgtct gccgatgagc
     1321 tgaacgttgt tgctggcaat aactatgtta atgccgcagg ccaggtgacc ggtagcgtat
     1381 ccgccacggg gtcccgtaac ggttacagcg tagatgttgc caaactgggc ggaatgtatg
     1441 cgaacaaaat cagtctggtc agcaccgaga aaggtgtggg ggttcgcaac ctcggcgtta
     1501 ttgctggggg ggttaatggt gtcagcatcg attccaaagg taacctgtta aacagtaacg
     1561 cccagattca gtctgcaagc acgatcaacc tgacaacaaa tggtactctg gataacacca
     1621 ccggtacggt gacatctgta ggcactatct cgcttaatac caacaagaat actatcgtga
     1681 atacccgtgc gggtaacatc tctacgatgg gcgatatcta cgttaacagc ggtacgattg
     1741 acaatactaa cggcaagctt gcggctgcag gaatgctggc ggttgatacc aataacgcca
     1801 cgctgattaa ctctggtaaa gggagttctg tcgggattga agcggggctc gtggcgctga
     1861 aaaccggaac gctcaacaac agcaatggtc agattcgcgg tggctatgtg ggtcttgaat
     1921 ccgctgcgct gaataacaac aacggtgata tccagaccac cggcgatatc gccattatca
     1981 gtaacggtaa tgtggataac aacaaaggtc tgatccgttc gtccaccggg catatcgtta
     2041 ttggcgcggc aggtagcgta aataatggtt caaccaaaac cgccgatacc ggcagttctg
     2101 actctctggg cattattgca gataccggcg tagaaattgg tgcgaacaac atcaataaca
     2161 acggcggaca gattgcgtct aatggcaacg tctccctgtc aagttacagc acgatcgacg
     2221 actatgcggg caaaattctg tccaacagca aagtgattat caagggaagc tctctgcgta
     2281 acgataccgg ggggatcagc ggtaagcagg gtattgaagt cgccgttggc ggcagcctga
     2341 ccaataatat tggcgtgatc agctctgaag agggtgatat ctccctgtta gccaactccg
     2401 tggataacca cggcggcttc atgatggggc agaacatcac gatggagtcg atgtctggcg
     2461 tcaataacaa cacagcgctg atcgtggcca gcaaaaaact gaagataaat gcgcgcggca
     2521 gtatcgaaaa ccgcgatggc aataacttcg gtaatgctta tggtctgtac ttcggcatgc
     2581 ctcagcaaac gggtggaatg gtcggcaagg aaggcatcga gctttccggg cagaacatct
     2641 ataacaacaa cagccgtctt atcgctgagg atggtcctct gactctgcag gcgcagaaca
     2701 cgttcgacaa cacgcgtgct ctggtcacca gcggggcgga tgcatctatt caggttggcg
     2761 gaacgtatta caacaactac gctaccacct ggagtgcggg caacctggat atcgacgcga
     2821 ccacgctgca aaacagcagc agcggtacga tgatcgataa caatgcgacc gggttcatag
     2881 catctgataa aaacctgtca ctggaagtgg tgaatagcct taccaactac ggctggatca
     2941 gcggtaaagg cgatgttgat gtcacggtga ataacggcaa cctgtataac cgcaatacca
     3001 ttgcggctga aaaggggctg gatattgccg cgttgaacgg tattgaaaac tggaaggata
     3061 tttctgctgg cggcgacctg acgatgaaca ccaatcgcca tgtgaccaac aactccaaca
     3121 gcaatatggt ggggcagaat attgttatta acgcggttaa cgatatcaac aaccgtggca
     3181 acattgtcag tgacgctgac ctgaacgtga cgaccaaagg caacctgtat aactatctct
     3241 atatggtagg gtatggggat atcgcattgt cggcaaatag cgtggcgaac aataacgcga
     3301 ccatcgaagc gacaggcgat ctgattatcg attcgaaggg taacgtgggt aacaaccgcg
     3361 gtaatctgca tgcgttgaac ggcgtgttgt ctgttaaagg caacaatctg aacaacgata
     3421 acggtgaaat tcgtggttat ggcgatgtca cgctggcact gacgggcaac tacgacagct
     3481 ataagggttc gctgacctct gaaacgggcg acgtgactct gacggcgaac attgtagaca
     3541 acgcctatgg tttgattgcc ggtgagaatg tttctgtcga tgctaaatcg acgatttaca
     3601 acaacactgc gctgatcgcg gcgaataaaa agctggttat taacgctggc ggcaacctcg
     3661 aaaaccgcga cgggaataac ttcctgcgta ataacggcgc gctgtttgga attaccgaca
     3721 acgttggcgg catcgtaggt aaagaaggtg tcacgctttc tgctcagaac gtctacaaca
     3781 ataacagcag catcatcgct gaaaatggtc cgcttaatct gctgtccagg ggaacgctgg
     3841 ataatacccg cgcgcttctt agcagtgggg ctgatgccat catccgtgcg gcagggacgt
     3901 tctacaacaa ctatgccacc acgtacagcg ccggtaatct cgacgtttat gcggcgtcgt
     3961 tgaacaacgc cagcgatggt cgcctggaag acaataccgc cacgggcgtg attgcgtctg
     4021 acaaaaacct ggatctgagc gttgataaca gtgtcactaa ctatggttgg atcagcggta
     4081 aaggagatgt gcatttcaat gttctgaaag gcacgctgta taaccgtaat gccatcgcgg
     4141 cggacaacgc gctgaccatt aatgccctga acggtgttga gaactttaaa gacattgtgg
     4201 cgggtactgc gctgactatt gatacgcaga agtatgttac caacaacagc aacagtaata
     4261 tgttgggaca aaccatcgcg atcaatgccg tgaatgacat taataaccgt ggaaatattg
     4321 tgggtgatta ttctctgggt gttaaaacca ccggtaatat ttataactac ctcaatatgc
     4381 tgagttatgg tgtcgctggc gtatcggcaa ataaggttac gaatagcggt aaagacgctg
     4441 ttctcggtgg cttctacggt ttagcgttag aagcaaacga aactgataac accggtacta
     4501 ttgtcggcat gtaagatgta accaaagaca gcagtggctt ttaatattaa tgcgtcatgt
     4561 cacgcagggg ttgctgctgt ctttatttag tttgagaaga taatgcaatt acgatttaat
     4621 tttggtttgc tgctcattct ttgttgtttt caggttgcag cgaaagacag tcttgataat
     4681 ttgatcaagc agcaaaaaaa tgcagatgaa agcacgttgc gcgcgcataa attagaaaaa
     4741 aaagatgttt actcaaatgc acaacgtaat gcgtttactc tgggtgatct gccgcaggaa
     4801 aagaattgct ttatcattaa tcggattgaa ctggaagata actttctggg tggtcagttg
     4861 atttctgaag tgcaaaaagc gattgccgga cgttgtgtcg gtagtgctgg tgtgcaaaaa
     4921 attgcagctg ccttgcagga ttattatatt aatgcgggat atatcacaac gcgaattagt
     4981 atcccttcgc aggatatttc taaaggcaag ctacgtttcc aggttaatgc cgggaagata
     5041 gaaaaaacga ttgtcgaagg taatgatatc agtgaatgga tgttaccttt tcagcgtaac
     5101 gatattctca atattcgtga tattgaacag gggctggaaa atcttcagcg agtacctgac
     5161 gttgatgtga aaattaatat tgaaccggga acacgggatg gttacagcat cgttcatatt
     5221 gatacacacc gggggaaaaa ctggagcgtt cgggcaagtt acaacaattg gggcgatgaa
     5281 gaaacgggcc gttaccagac aacctcggtg ggttatctgt ttaatccagc gaaaatgggg
     5341 gatctgtttt atctcgccgg tacgcgtagt actaccggac agtatgaaaa cgtcagtagt
     5401 tattattcca ttccggtggg gtactgggag tacggctttt tctatagcaa aagtaaatcg
     5461 cagcaggtga ttccgctttc gtacatctcg ctggattatg ttggcatcag cgaatacttg
     5521 agcgcaaaag cgacgcatac cgtttatcgc gataaaacaa aaaagttcgc cggtagtgca
     5581 gaactcattc gacgaaagtc gaattacacg cttaatggtg aggagctgac attacaggcg
     5641 cgggatatgg gaaatatcaa gctggggcta aattataagc aacagttacc cggcgcgttc
     5701 tgggattcaa ccctctcgtg gcaacgcttt ttaacctggt tcggtggtga aaaaacgccg
     5761 gatatgcaat atggcgacgt tagcccggta agcaatatct ttaattttga gggtaactat
     5821 acccgacaaa tagagaatgg atattacaat accgcttttt ttgcccagta cgcaccgcgt
     5881 gaactgactt tgcaagataa aattacagtt ggcgatcgtt ggagtattcg cgggtttgaa
     5941 aatagcgtag gtttgtcagg gaatgatggt ttttatataa aaaatacgct ggcatttcct
     6001 ttgcctggaa tgaaagctaa ttattatgcc ggtcttgatt ttgggcaagt gtatcaggac
     6061 gcttcttatg gcgatgaatc actggtgggc tcggcagttg gtattgatgg aaacattaaa
     6121 agtcttgagt ataacttttc cgtttctacc ccattaaagt atccggcgac attagatatt
     6181 gatcgggtga atgtaaattt taatttttcg taccaaatgt aaaggataac gagactcatg
     6241 agaataggga cagatattgt tgaaattgcc agaataacca aagccattat taatggagag
     6301 atggcttttt atgagcgtgt ttatacttct catgagcgct tactcattaa ccctgcagaa
     6361 cctgattatg aacgggcctc tggattctgg gcggcgaaag aagcgttagt taaagcgatt
     6421 ggttgtggtt ttcgtgatgg cgtacgtttc caggatattg aaataaagca tgatgcagcc
     6481 aaagcacctt atttcgttat ttccggaaga ctacaagaaa tttttatcgg gcaggggttg
     6541 caaaataact ccctgagtat ttcgcattgc cgtacccacg ccattgcgac tgtcatcgtt
     6601 tattaactca acatatgtga tgaatatgta caaatataat gatttaaatg aaaaaaccgt
     6661 actggtgaca ggagccagtg gtgatattgg actgggtatt tgcgaaaaat atctggagca
     6721 gaactgcgat gtttatgcgc tctataaatc caacgacgtg caattaacgg cgctgaaagc
     6781 aagccatcct gcaggcgata aactgcatat tgtgcagtgc gatcttgcct gcccccagag
     6841 cgtgagcgca ttgtgcgaac agatagagcg ccaagctggc aagattgatg tgctggtaaa
     6901 taacgcgggc atcgttaaag acagcctgtt tgcctcgatg agttatgagg atttcaccca
     6961 ggttatcgaa accaacatgt tcagcatttt ccgtctgacg aaagacgcgc tgatgctgct
     7021 tcgtgcggca gaaaatcccg ccattattaa cgttgcctct attgcagcgc tgatcccaag
     7081 cgtcggtcag gcgaattaca gcgcgtcaaa aggggcgatc ctaggcttca cccgcacgct
     7141 ggcagcagaa atggcaccct ggggcgtgcg cgtcaacgcg gtcgcaccgg gaatgattga
     7201 atcgaaaatg gtgaaaaaag tatcccgcgc agtggtccgt gcggtaacca gtaccatccc
     7261 gttacgccgt ctgggtaaat gtgaagaagt ggcgaacacc atcgtcttct taagttcctc
     7321 tgcctccagc tacattgtcg gccagaccat cgttatcgat ggcggcctgg tcatgcggtg
     7381 atttatgtct aaatacacga ttccttcgaa aatcttcctc gaaatgggcg gctggcgtca
     7441 gccgctgctg atggtcgata aaattgcaga ttataaatac ggtgaaaatg gctttgtcag
     7501 tgtcgtgaaa cacgtcacct ataacgaacc gtatctactg gggcatttcc cggaagatcc
     7561 gattatgccc ggggtgatta tttcggaaat cttcggccag gccagcgagt atctctcttt
     7621 cctgaccgac atttgcgata tctggcgcga acgttttgag gaagagctga agtcgttacg
     7681 tgatattcac gcacgcattc accgccctga aatgctggag attatccgta cccggcgcag
     7741 ccaggtacgc ggcgtgctgg cggctcagaa cctgaaattt aaagatatcg cctatccggg
     7801 tgattctatt gatgttgtca gcaaattggc tttttcggat gccagtggct ttaaacacta
     7861 ttccgtgacc gcgtacgtcg gtaaaaagct gattagtcag ggcaccatta tcaatttccg
     7921 tgaaaccaag taatccaata agagagtaaa tattatgtct accatgaatg acgctattga
     7981 acagcgtaaa gaagttctga tgaccattaa aactgagatc atccagcgac tgaatattca
     8041 acgcgatgaa gcgcaaattg atgatgatac tgcgctgttt ggtaatggtt ttaaactgga
     8101 ctccgtcgat gcaacggaaa ttgtcgttct gctggataaa gtatttgata tcaagatcag
     8161 cgaaagtgac gacccgtcct atatgcgcag tatcaatagt ctggccacct tcgttattca
     8221 aaagaaaaac gattaactac ttattaaaga attaattatc aggacacgtt tatgaaatct
     8281 cttgctgaat ttcacttgaa aaataatgcg gtaatggggg tatataacaa ccgtacccta
     8341 ccttcttctt atcacgatgc tatgaccgaa tataaagccg ttcgtgaaaa cgcgctgctg
     8401 gtggactatt ctcacctctc tattgttagc gtcatgggcg atgatgcctg ggcgctgatc
     8461 aaccagctgg tttccgcaga tgtctccatc attcgtgatg agcaggcaat ctactcactg
     8521 gtgttgaatg aagagggcac cattcgcgga gacgtctatg tgctgtgcag cattgacggt
     8581 tattacctgc tttcagaaga tatttcggct gccgaattga tcgccagcat gaataccatt
     8641 ctcgaaaaag cggaagaact ggatattcaa tcgatgcctg aaattcagga tatgcgggaa
     8701 aacaactggg gggcaatcct gctcgaaggc ccatactcct gggagatcat gtctgaaatt
     8761 catggctttg acgtgattgg cctgccttac tacgaataca tgaataccga ggaggatctg
     8821 ctgctcttcc gctgtggtaa acacggtgag tatgcgtaca tgaccatcgg tgagcaggcg
     8881 aaactggcgg agcagtggga aaaactgtta accgttggcg agaaatacct gatgcagacc
     8941 ggcggcctgg attatcaaaa aatcgtacgc ctcgaaaatc cgtgctggga tgccagcctc
     9001 tgggaaggtc aggcggtgaa tccggttcag ttgcagatgc agtgggcggt gcagtacgac
     9061 aaagatgatt ttattggcaa ggacgcggtg acagagctat cccaggagta taccggtaat
     9121 aaactcatcg gcatgatcgc acaggaagaa tgcgaaggca ttgaggctgg cgatcgcgtg
     9181 ctggtggaag gtcaagacgt gggctatgtc gtgaaggcac tcttttcccc agcgttgcag
     9241 cgtttcattg ccctgaccct gctggaaaag gattacgcct ggtctgacat cagcggctac
     9301 gaaattcaaa ctgctcacgg aattattccg gcgcaatcaa aatgtatgcc gtttatttat
     9361 aacctgagca tgttggttag cccaactgag cacagttata tcgacgcgtc aaaaaataaa
     9421 agcgcagcgt aatgaatatc gccgcgtgac tatagcttac gcggcttaaa ttaaccccaa
     9481 ttggcgggat tgccaggtat tattcatgaa aacgaacagg aaaagagttg ttattaccgg
     9541 gctggggatt ttgtcctcac tggcggaaaa tattcaggat ttccgggaag cattattaaa
     9601 taaaaaaaat ggcgttaagg atagcgaacg ctttgcaaaa tggtttgaaa atgcgcgtgc
     9661 ggcagaagtg ttgcatgaca ttgaatactc cgatctgaac gaagatgtta tcgattcact
     9721 ggataacgca gcgctctggg cctacaaagt gggtaaagat gcgctgagtc aggccggcct
     9781 gatggatagc cgtgacaccc tcaaagaaac aggcatgatc gtcggtgttt cgtccgcggg
     9841 aacagaggca tttttgccgc tgttcgaaca gcgcatggaa gatttctcgc tgcgtaaagc
     9901 actcttttcc ggcgcattct catcctgttg ttccagcgtt tcaacgctgc tgggcttaca
     9961 gggtggggtt gaactggtgg cgaccgcgtg taccgccagc ccgaacgccg tggggatggc
    10021 ctttgactac atccagaatg gtaaaagcaa aaccatgctg gcagtgggca ctgagcccat
    10081 ctatctgccg acctttgccg gattctacgc actgaatgtc atgcatccag acacttgtac
    10141 gccgttctcg ggtcagtccg gtatgtcgat tggtgaaggc gctggcgcta tcgtgctgga
    10201 agagtatgag cacgccgtag cacgcggggc gaccatttat ggtgagatcc tctcttacgc
    10261 cacttcctgc gatgctttcc atgaaaccgg gccagatccg cgtgccagcg gagcggtgca
    10321 ggtcatgaac aaggcaatgg caaatgcggg cgtaaccccg gagcaaatcg attacgtcaa
    10381 cgcgcacggt acgggtaccg aagccaatga ccgtattgaa acgctggcaa tgaaaaaggt
    10441 cttcgctaac cacgataaac tgctggtgag ctccaccaaa tcttactttg gccacaacat
    10501 tggcgcagcg gggattgtgg aactgattgc ctgtctggca acgcttccgg ataaccgcgt
    10561 actgccgacg ctgaatttca gccatgcacg cccgaattgc gatctggact atgtgccgaa
    10621 tgagtttcgc gatcgcaaag tgaacatctt catgaagaat aactatgcct tcggcggcaa
    10681 caactgctgc atgatcctga gcatggagcc cgcgtccatt ccggtcagta cctatgattc
    10741 gaagcgtgtg gctatctcgg gcgtcggtgc agtatccgca ataggccaca cgctcaacca
    10801 ggttctggaa aacatctggg cgcaggacca ccacgttgaa ctgggtagcg taaccttccc
    10861 ggacgataca ctggaagaag ccaaagagct gctggccgtg ctggatgaat caaaccagtt
    10921 tgccgagctg tttgatgaag agtacagctt cagcgaagct acgcttccgg aaacggaaag
    10981 caactttaaa acgttccagg tttccgggct ggagccgcgt aaacacctgc gtcgtttcga
    11041 tcagcgtaaa gcgacccgtg gcggcacgtt tgccctgatc gcactcaccg aagcgctgga
    11101 acaggcgaag cgtaaaatca aacgcgacgg cgatgagttg ggcatgatca tggggatgtc
    11161 ccgcggcccg caggaaacca cctacaaata tctgcaaagt ctgaaacccg atccacgcaa
    11221 agtacgtaca tcggaatttc ctggctcgct gatgaatgcg atcccaacgt tctgcgggat
    11281 ctctgaaggg atcaaagggt ataccactac gcttgcgaca ggggaaaact cggcgctggg
    11341 cgcgctgacc tacggttatg aaatcgtccg gcagaacctg caaccgcagg tattggttgg
    11401 cggcgcggat gagtacttcc cgtcgatgtc gctgtatatg gatgcggtga cacaaaagat
    11461 ccacatgacg gctgaggcga gcgactatca gatttacggt aaagatccgc aaggttatgt
    11521 tccgggcgaa ggggcttgca tgctgctgct ggaagatccg cagttggctg tggcgcgtgg
    11581 cgctgaggtt ctcgcggaag tggtgggcta cggcaagtca tgcagcaata cctacttcga
    11641 cgcctcattg ttggaagaaa aatccgcgtc gatggcgctg gccattgaac gcgcccttac
    11701 gcatgcgggc atctctgctg gcgatattga tttggtatgt ggtaccagca acggtagcga
    11761 tgaccattct cgtatcgaaa ttgacgcgat ttacaacacc ttcgccgcgc aaaacccggc
    11821 ggtgccggta gtgaactata acgcttgctt tggttttgtc gcctctgccg ctggtttgct
    11881 taacctcgcc gtgattactg actgcattaa gaagcaggca gtacctgcga ttccaaatac
    11941 ggtggaattc tttgatgagc gtgtgaattt tgtacgccag tcgatgaaac ttgcgctgaa
    12001 ccatgtcctg cttgttggcg caacggaagg cggcaactac tacgcgttcg tcattaaggg
    12061 ataagactgt ggacaatgcc gctattattt cgggttacag cgtgtggatg ccgtatgccg
    12121 aagactgttc gcagttaatt gacaatctga aacaggggaa acgcgttgcg cgtacaccct
    12181 ggtttacctc caacgaggaa gccattaaat gcgggtttaa gggtaatccg tcagtggcga
    12241 cgttgaaaca ggtggacgat agcgcattgg acctgctgtc tcagttgatc gacgaagccc
    12301 tggaacaggc aatgctggat aagcattgcc tggccgggcg caacgttcgc gtttatctga
    12361 ccggtattgg gccgcgtatc gacgggctgg attacaaatc tttctataat tacaacgatg
    12421 tagaagatat caacttaacg caatccatca caaatctgca tgcctcaaag atgtcgcagg
    12481 acacgatttc aagccatctt gcgcgcaaat atcgcttgca gtatttgccg cctaacatga
    12541 actgcaccag taactcgtca ctgactgcgg tgcatctggc gacgcagggc attgagcagg
    12601 gcgggattga tctcgcgatt gtgctgaact gttcgaaaat taaaacccag gacatctggt
    12661 tcctggaaac gcaatcgatg ctggacagcg aacaagtgca gccgtttggt gaaaacagta
    12721 aaggtgtggc gttcgcagaa ggttttagcg cgctcctact ggaaagcgct catcaccgcc
    12781 gggcgcgtca acaaagtgag ggcgtaaggg tgcaaacgac ttacacccaa atcagcgcgg
    12841 gccgcagcaa cgatgcctcc tggcttagca ctaacgtgca caaggtcatg caggccgcga
    12901 tgaagcaggc tgaaattgcg ctggatgatc ttgcagccat tcttccacac ggcaacggtt
    12961 cggcagtgag cgataacgcg gaggccaaag ccatcgccat gttcgcaggg gagcgacaga
    13021 tccccgttct cgcctataaa gggcagatcg gctataccgc aaccggatct ggtgttgtcg
    13081 atctgatcat tggccaccat tcgttgacgc atcatcaact gatcgcacct gttggcaacg
    13141 acgtcattat cgacagcatg gcttcgctgg tacttacgga cggcagcgtg acaaaccata
    13201 gcaaacgcca tttgctgaag gttggtgtgg gtgtcgatgg ttcagttatt ggcgttgtca
    13261 tgacaaatat gcaagcgggg cgcgcgaaat gacggaaaag agtatctacc tgtcttcctg
    13321 ggcgcttacg gaaccggacg acatttttta tttttatcag ccgacctggc tcaacgcctg
    13381 ggaagcacat atgtacgaaa ggggattata cggcacccgt gctaatggct ggggactgaa
    13441 gcgctttggc gtggagaacg atatttatgc accagaacgg atccgtaatc cacaatatat
    13501 gccttacctt gaggcgatgg agcaggtgac cgattctgca ttacaacaaa tgccgactaa
    13561 agaacgcatg aagttaatga aatcgcgcac ggcatttatt tatgccgatt cctggggcga
    13621 atccggttcg tttgaaaaaa tttccagtgc gctgcatatc gccactattg atacgcttcc
    13681 caagaattta cttaagaagt tttcgataac ggaaccgact tgcaagattc gcggcgaaaa
    13741 acaggcattc gttcaggcgc tgcgtatggc gcaggattat ctcgcctggg atgtattcga
    13801 taacgtggtg atttgtgctg cctatcgggc aattccggtg ctggtattct ctgaagaaga
    13861 tgtgggtaaa ccgcctaagc gtttatttcc ccgtcgtgct gatgaggtga atttatctgt
    13921 tgaacgagtg ggttgtttta ttctcagcca gcgtgagagc gccatcaggc tgcaatgcgg
    13981 ggaatatcat tccggcactg cgccacagca tgctacggat attgatgtca tggccttcag
    14041 cgaacggcaa caaatgccgt cccggcctgc tgctggcgtt atcgacctgg tggcccatta
    14101 cggtgaaagc ggttgtctga cgcccgcgtt gagtatggat tatttaaccc gtcatattca
    14161 gcccggtgga aaaatgcgta cggccattgc cgataaacag tttggttatc actactttga
    14221 ccttgaatac cttgaaggat aacgacagtc gtatgcaatc tactattaaa tcgttgcccg
    14281 ctatttcgct tagcaatatt tataagtgtt atggcgctgg ggcaggaaag gttgatgcgc
    14341 ttaaaagcat taatcttgat atcaataaag gcgaatttct cgcactgtgt gggccgtcag
    14401 gcagtggtaa aagtactctg ttgaatattc tctcgggtat tgataaaccg accagcggaa
    14461 cggtgatatt tttaaataaa ttactgaacc aactgccaga agagcagttg gctgtgattc
    14521 gtggaaaaca tttgggtttt atttttcagt tttttaacct tatgccggta ttaaatgttt
    14581 tcgataacgt ttatttccca ctggtgctta atggtcagtt cagtaaaaaa gaagccactg
    14641 aacggacgct gcattatctc gatagtgttg gattaagcgg ttttggcgat cgtaaacctg
    14701 ggcaactatc gggcggtcaa caacagcgtg tcgccatcgc cagggcactg gcccacgaac
    14761 cgatggtggt cattgccgat gaaccgacgg gaaatttaga cctggcaacc ggcgaggcta
    14821 ttttggacct gttgttgacg attaatcagc agaccggcac cacatttgtt atttccacgc
    14881 actccagcga attaaaagcg cgggcacggc gagtggttga aattcaggat ggagtattga
    14941 ttcatgactc agatgcataa aggatttttg ctcatcggcg tctttttgta cgcggcgttt
    15001 gtggctccga agatttacgc ggcaacaccc gacgctgtta accaggttct gcaacagggg
    15061 ctcccggcgc agcctgccgc gctggcgacg ctgaccgagg cgttaaagaa agagtatgaa
    15121 gccaggcaca atgtggtggc gctggttttt tacgcctatg gcttactgcg tcaagcggac
    15181 ggttatgcgg caacaaacga ttttatccat gcctcagaat acgccaaaag cggctttttc
    15241 tggctggatg aagcggtgga tttgcaggag aaaaatcaac gcgtacggta tttacgcgcc
    15301 cgcgtagatg cctatttacc cgcagactca gggcgttgcg tggtgacagt caaagacacc
    15361 gaacacatgc tggctgaccc ggcgatatgg acgacaacga tccgcgacca tattctggcg
    15421 atgcgttatc gcgcattacg tcactgcaag gacaccaccc gcgcgaatgc gttgttggcg
    15481 caaattaaag ggcaaaacgc cgcgcttgcg cagtcgctga cgcaggattt taatgttgta
    15541 ccagaatggg atagtgaaga gttaacccag gtactgctgc cgctgatgaa aggtgagtaa
    15601 atgtccgggg aaattttagc tgttaattgc gttctgctgc tgattctgtt ttttgtgttt
    15661 tatcagcttc tgttttattc acgagatgtc aaatttgcat ttttgaatct ctttcgccac
    15721 aagcgtcgct ctttttctac cataaccgct attgtattgg gtggcgtggc aattttttta
    15781 tatggcggat ttattgatta ctctttctgg atcctgaaag agcagacgat tcgtaccaat
    15841 attggtcatg tacaaattta caatcagcac tattttgaaa cgtcgaataa aaataagagc
    15901 ctgattgccg attatgcggc gttaaagaaa gctattttgt cggacccgac gctggcggcg
    15961 gatatttcca cgctctccgg gcaactggag tttactggcg taatttcaca ttacgagagc
    16021 gaaacctcca gctatttctc cgcgctgggg gtcgagccgt tacccgcttt aaagctgggt
    16081 tcgttcgata agattatcgc ggggagcgat ctttcccggg taaaaggtga tgaaatcacc
    16141 ctgggcagcg gtctggcgaa aacgttgaat gcgaaatatg acgactggct ggatgtgatg
    16201 gtggtcaaca cggcgggcgg gcagggcgca ttgtcgctaa aaatgcgtgg catttttgag
    16261 tcaggcatta aagattatga cgatgtcgcg atgaagatcc cgctggacac cgcgcagcgc
    16321 atgatgggta cggatggcgt cagcaaagtg cttattctgc ttaaagagga tgacaccgcg
    16381 gcgtttagcg caaagctgcg tcaatttatc gaacgcaatc atttgccgct ggtggtcaaa
    16441 gactggcgtg atgtttcact cttttatcaa caggttgaag ggttgctatc ggggatctat
    16501 ttcttcatca agctgattgt tgcgctgatt gtcatcttca tgatcggcaa ctcgatggca
    16561 atgaacattg ttgagcgcac ccgtgaaatt accactctgc gtgccatagg cctgaagccg
    16621 cttcacgtca cgcgcttgtt tctgacagaa ggcattttta ttggtgtgat tggcgcagtc
    16681 ggcagcctgc tggtgggggg cgtgttagcc tggattatta atctttatgg tattgcgatg
    16741 ccgccatcgc cgggacaaac catcggctat accgccttta taaaaaccag taacccggaa
    16801 ttgatttggg tgacggtggt attacctatt ttgacagcaa caggtgcctc tgtgttaccg
    16861 gctttacgtg cttcccggct gaatatttct gacgcattta aattctcata gggctaatta
    16921 tgaatatggc tttttacggc aagtggtttg cttgcctgtg gctcgctacg tcgtgcgttc
    16981 aggcggcgag cacagataat aaggcgctgg agataattcg tcgcgctgac gaaattcgtt
    17041 cgccgaataa accgtttcgt tacacgctga cggtaacgga atataaagcg ggcgcaaccc
    17101 agccagagaa taaacaggtc ctcgatattt ccatgcgctt tatgaaaccg cagggtaatg
    17161 aaaaagcgga tgcacgctcg ctggtgcgtt ttatttatcc gccgcgagat aaaggaaaaa
    17221 tcatgctttc cgactggtac gatttatggt tttacacgcc ggaactgcga cgtcctatgc
    17281 ctatttcacg gcaacaaaga ttaattgggc aaatctctaa tggcgatgtc attgtcacta
    17341 actttgaata tgcctatgac tcgaccctga tgggggaagt cacgtgtgct gaaaagcagt
    17401 gttacaagct ggcgctggtg cgtaaatcgg cggatatcac ctggccgaag gttatctatt
    17461 acgttgaaaa agacggtgat aatcgcccgt ggaaggcggc ctattattcg caggatgacc
    17521 agttaataaa agaagtgttg tatcaggatt tccagccggt gttggggaaa acgcgaccca
    17581 tgaaaataac cgttaccgat gttcgtcacg gcaataacta ttcagtgatg gagtacagcg
    17641 atgttcgtct ggaatcgctg ccggagtttc atttcaccaa agagtatatt cagcgaggtg
    17701 cgaaatgaaa cgtacgctga agatctcttc gttgctgtgt gttgcgctgc cgttgacggt
    17761 acaggcggat tgcctgtcag gtgacgaggt ggcacaaaat agcgatgtga cgctttacga
    17821 gcaggtgtct tatgtcaata aacaggcttc ggcctggcag attgcaggca aaaatcccta
    17881 cacccgtcac aacggttatc aggaggcggg gattggcatc aatagcggtt gttcgataat
    17941 cgacaatacg ctggacctta agctcaatct gtatggaata aacgaatatg cgcttaaacc
    18001 cgcagggaaa ttcgaaacgg atgatagccg cacgcgtgcg ttaattaacc gcctcagtct
    18061 ggtctatagc gcctcggaca gcgttcaatt tgaagcaggc aaatttgccg cgccgtcagg
    18121 aatgttcttt ttgcgttcgc cttctgattt gcaaacgcat tattacaccg gattccagtc
    18181 gacgcgtttg cacgatccca aaatgacatc ggcgtatcag gcgtcatcgt ggggcgcaaa
    18241 aatgagcgtg gatacacgcg attatgcgtt ttcggcgagt gtcataccga agttagccac
    18301 catcgacaaa cgctatgtga cgtctggcaa ctggtcggct aatcagcagg gaaacagcga
    18361 cgaagcctat ctgctgagct tcagcgatca tcgctttggt gagcacacgc cgacagtcaa
    18421 tgtgcggctc ggaccatcgc cgtcactcgc gctgtctgac agttatcact acaccccgca
    18481 gcttacgctg aatgtcgacg cagcctacca ccgctcccag caatggcggc atctttccca
    18541 ccgtgagacg gcgcaggtcg aggaatatca gtttccggat tcgctctatg aaacgaaaga
    18601 tgaaagcggc gtagagctgg cgctgggtgg gcagtacacc agtgataatt tcagcgtgtt
    18661 tggcgttgag tattactttc agagcgaggg ttattcgcgc gctgaacagc gtcagcagcg
    18721 cgagttaatt gacttcctca ataccacaac cggctatgcg ccactggatc aggcatttga
    18781 ttcttacaaa tacctgatgg cttcagaaat cagcaatacc gccaatcagg ggatgctgca
    18841 gggtaagcac tatctgaacg cctgggccag tctgccgctg gcgggagagt cgacgctgca
    18901 gccttcgctg gtggtgaatc tcgttgatgg cagcacgttg ctgggcctgc attattccac
    18961 gccgctcagc gctataagta accagcttga agcctatgcc ggtggctata gcgcgctggg
    19021 aagtcggtac tccgaatttg cgctttttgg cgacacgtta ggcctttatt tgggctttaa
    19081 atactatcta taaggataaa gagatgatga ataatgcttc acaaccgaca gtactgcttt
    19141 ttgccggtca gggtaatccg gttattggta tgggcagcga tctttgggat ctcaacgcca
    19201 gtacaaaaca tatctgggat tgtgccagcg atatttccgg actgaacctg cgtcgcctgt
    19261 gtcagaaagg gccgatgaat cgccttattc aaacgacggt acaacaactg gcggtgaccg
    19321 caattaatgt aacgctctat accctgtgcc gcgagcgtct tgagaattta acggttgtgg
    19381 gctcctgcgg acacagcgtt ggtgaataca gtgcgctcta cgccgctgga gctatttctc
    19441 ttgagacgct atttggcatg attcacttca gagccagcct gatgcatgac gaaagccaga
    19501 gaaataaagg cgcaatgctg gcggttaaag gcatcaaccg cgaacaactg cagacgatga
    19561 ttacggaatc aggcattgcg ctggatatta gctgtgataa cacgccgcgc cagcaggtta
    19621 ttgggggaac gcaggctgcg ttgaatgaat ttgccacgct gctgatggcc gctggatacg
    19681 aaccggttaa actcggtgtg agcggtgcct ggcacacgcg tttgatggaa gacggcgttc
    19741 aggcaatgcg cgattatctt gccggactgg atatcgcttc gccagagcat caggttctga
    19801 tgaacgtaac agcaaaaagc gaggtcgctc cgtcaattat caaagaaaat ctctcattac
    19861 acttaactca tacggtgaaa tggactgaat ctctcgatac atttctgaat atgccaacct
    19921 cggtagcttt ccttgaaata agtaataagc cctatttggg gaatatgttc aacgatttcg
    19981 ctggcgtgga tcagcaacga gttatgcatt gtcgtaaagc atttagtgat gcgaaggtat
    20041 tcaaatgatt cctgattatt taacttttat tcgctttcag gataaacgaa gtctgatata
    20101 catttatgct attggactta ttctgatagg cttttattgg aaaaatgcag gatttacttt
    20161 tccatcagag gatattggtg tcgttagtgg gattctggcc ctggtgctgt ataattttat
    20221 ttttgatctc aaggcgtact gggcttataa atgcgtcacg aagaatatcg atttttcgtg
    20281 gtttaagaaa aagcagaacc acaaaataga attatttctt acgcaacctc tggtggcagg
    20341 atttctgtcg ttaatcatgt tgagtgcaat gagttggggg ctataccagc gtctaccctc
    20401 gttatatgcg ctgttcctga tttcgttact tgggccgttg gttatctttc tgctgtttcg
    20461 gatgatccgc accagttatg tcaagcaggt cgctatttca gtagcgaaaa aagtaaaata
    20521 taaaagtctg actcgctatg tgctgctttc ggtgtgcatc tcaacggttg ttaacctgct
    20581 tactatcagc ccgttgcgta acagtgattc ttttgtgata gaggggcagt ggttaacgtt
    20641 taaatcgata attgcattgc tcattctttg tggcgtagtg ttggcgatta atctgttttt
    20701 tctgcgcttc tccaggcggt acgctttttt gggcaggctt tttttgcagg aaatcgatct
    20761 ctttttctcc agtgaaaatg cattgtcgac cttttttgcc aagccgcttt ggcttcggtt
    20821 attcatattg ctggttattg aagtgatgtg gattacgctg gtgtcggtat tggcaacgct
    20881 tgtggaatgg cggatttggt ttgaagccta ttttttactc tgctatgtac cgtgcttaat
    20941 ttactatttt ttctattgtc gattcctctg gcataacgat tttatgatgg catgtgacat
    21001 gtatttccgt tgggggcatt ttaataagtg aggaagtgat aggaagtgac cagataatac
    21061 atatatgttc tgtactctct tgcgcatttt gattgttgac tgagcaacca gacagttgat
    21121 gtgcacgatt tcccctcgcc caaacagacg tgggcggggg cacccggtgt gtgcaatata
    21181 cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa atatggcggt gagggggacc
    21241 gcctgaaata aatctgactt aatccagggg tgttatataa tttcattata aacaatgtat
    21301 tacacttttt gttgtccgat attatctaat gtattcttag tgaagcgaga gactaatggc
    21361 atactggatg gcataccaga gtgacgggaa ttagcgtatg gcagtattga cggatacgaa
    21421 agcaagacat atcaaacctg atgacaaacc attgccccat gggggaatta ccggactgac
    21481 ccttcatcct tcttcagtaa aggggcgggg gaaatgggtt tttcgttatg taagtccggt
    21541 gacacaaaaa agacgtaatg ctggattggg aacttaccca gaggtcagta ttgctgaagc
    21601 tgcacgtact gcccggataa tgcgagagca acttgctgca ggtgatgatc ctctggagat
    21661 taaaaaggct gaatctgaga aagtcgctat cccaacattt gccgatgcag ccaggcgtgt
    21721 acatgcagaa ctgtctcctg gatgggaaaa tccaaagcat gtaaggcagt ggttatcgac
    21781 gcttgagaat tacgcgtttc ctcaactggg agcaaaaacg ctggattcga ttacggctgc
    21841 ggacgtggca gaaacactgc gtccagtctg gttaaccttg tcagaaacgg caagccgggt
    21901 taaacagcgc attcatgttg ttatgcagtg gggatgggcg cacggttttt gtgtagcaaa
    21961 tcctgttgat gtggttgacc atttgcttcc tcagcagaca agaggacgtg atgaacacca
    22021 acccgcaatg ccctggaggc agttaccgct ttttgtggcg accagtgtgt ataccgatga
    22081 accttataat gttacccgcg cactgttatt aatggtgata cttacagcaa ctcgctcggg
    22141 cgaagccagg ggaatgcgct gggctgaaat tgattttcat aagcgggtat ggactatacc
    22201 tgcagaaaga atgaaagcca ggatacagca tcgtgttcct ttatcccggc aggctattta
    22261 cattctggaa aatatacgtg gcctgcatga tgaactggtg ttcccttcac ccagaaagca
    22321 gcagatcctt tccgatatgg tgttgacaag ttttctgcgt aaaaagaaag ccgtcagtga
    22381 cattccgggg cgagttgcca cggcacatgg ttttcgctca acattcaggg actggtgtag
    22441 cgaacagggg tattcgcggg atctggcgga aagggcgctc gctcatacgc tgaaaaataa
    22501 ggttgaggcg gcatatcatc gtactgatct actggagcag cgtgtaccga tgatgcaggc
    22561 atgggcggat tatgtgatgt ctcaaattgt gaataaataa tagtgaaaat gtatattgta
    22621 cgatcatgat ttgatatgtg atcgtagcag tagtaattaa gaaaccgctt ttatgtcaaa
    22681 tgtattgagc tgataattat cagatcgtca ggaaagcgta ttttgattaa caggggatta
    22741 cttaacaatc ccctttttat aaaaaataat ttgcctgttt aactcttcac tcctgctgag
    22801 actgaattat tcctagtgcg tcataaatca gtcttctgcg tagtttattg tt
//
LOCUS       DAZGXS010000071        22720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGXS010000071 DAZGXS010000000
VERSION     DAZGXS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(510..1601)
                     /gene="ychF"
                     /locus_tag="ACHKG5_002848"
     CDS             complement(510..1601)
                     /gene="ychF"
                     /locus_tag="ACHKG5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0868918.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1718..2302)
                     /gene="pth"
                     /locus_tag="ACHKG5_002849"
     CDS             complement(1718..2302)
                     /gene="pth"
                     /locus_tag="ACHKG5_002849"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0868919.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2580..2858
                     /gene="ychH"
                     /locus_tag="ACHKG5_002850"
     CDS             2580..2858
                     /gene="ychH"
                     /locus_tag="ACHKG5_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0868920.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2913..4592)
                     /gene="dauA"
                     /locus_tag="ACHKG5_002851"
     CDS             complement(2913..4592)
                     /gene="dauA"
                     /locus_tag="ACHKG5_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0868921.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4717..5664)
                     /gene="prs"
                     /locus_tag="ACHKG5_002852"
     CDS             complement(4717..5664)
                     /gene="prs"
                     /locus_tag="ACHKG5_002852"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0868922.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5815..6666)
                     /gene="ispE"
                     /locus_tag="ACHKG5_002853"
     CDS             complement(5815..6666)
                     /gene="ispE"
                     /locus_tag="ACHKG5_002853"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0868923.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6666..7289)
                     /gene="lolB"
                     /locus_tag="ACHKG5_002854"
     CDS             complement(6666..7289)
                     /gene="lolB"
                     /locus_tag="ACHKG5_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0868924.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7503..8759
                     /gene="hemA"
                     /locus_tag="ACHKG5_002855"
     CDS             7503..8759
                     /gene="hemA"
                     /locus_tag="ACHKG5_002855"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0868925.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8801..9883
                     /gene="prfA"
                     /locus_tag="ACHKG5_002856"
     CDS             8801..9883
                     /gene="prfA"
                     /locus_tag="ACHKG5_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0868926.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9883..10716
                     /gene="prmC"
                     /locus_tag="ACHKG5_002857"
     CDS             9883..10716
                     /gene="prmC"
                     /locus_tag="ACHKG5_002857"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0868927.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10713..11105
                     /gene="sirB2"
                     /locus_tag="ACHKG5_002858"
     CDS             10713..11105
                     /gene="sirB2"
                     /locus_tag="ACHKG5_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0868928.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            11109..11918
                     /gene="sirB1"
                     /locus_tag="ACHKG5_002859"
     CDS             11109..11918
                     /gene="sirB1"
                     /locus_tag="ACHKG5_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0868929.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11954..12808
                     /gene="kdsA"
                     /locus_tag="ACHKG5_002860"
     CDS             11954..12808
                     /gene="kdsA"
                     /locus_tag="ACHKG5_002860"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0868930.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12956..13063)
                     /locus_tag="ACHKG5_002861"
     CDS             complement(12956..13063)
                     /locus_tag="ACHKG5_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0868931.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13492..13599)
                     /locus_tag="ACHKG5_002862"
     CDS             complement(13492..13599)
                     /locus_tag="ACHKG5_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0868932.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13998..15098)
                     /gene="chaA"
                     /locus_tag="ACHKG5_002863"
     CDS             complement(13998..15098)
                     /gene="chaA"
                     /locus_tag="ACHKG5_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0868933.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15368..15598
                     /gene="chaB"
                     /locus_tag="ACHKG5_002864"
     CDS             15368..15598
                     /gene="chaB"
                     /locus_tag="ACHKG5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0868934.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15759..16454
                     /gene="chaC"
                     /locus_tag="ACHKG5_002865"
     CDS             15759..16454
                     /gene="chaC"
                     /locus_tag="ACHKG5_002865"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0868935.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16498..16851)
                     /gene="ychN"
                     /locus_tag="ACHKG5_002866"
     CDS             complement(16498..16851)
                     /gene="ychN"
                     /locus_tag="ACHKG5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0868936.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17037..18431
                     /gene="ychO"
                     /locus_tag="ACHKG5_002867"
                     /gene_synonym="iatA"
     CDS             17037..18431
                     /gene="ychO"
                     /locus_tag="ACHKG5_002867"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0868937.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18432..19082)
                     /gene="narL"
                     /locus_tag="ACHKG5_002868"
     CDS             complement(18432..19082)
                     /gene="narL"
                     /locus_tag="ACHKG5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0868938.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19075..20871)
                     /gene="narX"
                     /locus_tag="ACHKG5_002869"
     CDS             complement(19075..20871)
                     /gene="narX"
                     /locus_tag="ACHKG5_002869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP0868939.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21210..22601
                     /gene="narK"
                     /locus_tag="ACHKG5_002870"
     CDS             21210..22601
                     /gene="narK"
                     /locus_tag="ACHKG5_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0868940.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5580 a         5660 c         5879 g         5601 t
ORIGIN      
        1 ttttcgcttg atgtatctgc ataacaaaaa caatatgcgc catttttgca ttactaataa
       61 gaaaaagcaa aaaacatcga tattcatttt tggtcaatgg gttttctggt ctatcgttgt
      121 tattgtgcta ctggttatca ttttcatctt gccaggatcg cacgtaatca cgttattcgg
      181 ggactttatc tgcgggatat tacccttcag gtaagtcctt ttctcgccag catattggag
      241 cgtggtcaat attgatcggc tgtgaagggg gctgatgtgt agtggaacaa aaactgacgt
      301 tatatcactg ctaatgagat atccgtcatc tgaacactga tgtctttaat tattctcgtg
      361 gttcactgta ggcaataagc acaaaagtgt aggatgttac aagaatgatt aggactcggt
      421 gaaatgaaaa atccacgcaa ttgcgtggat tttatatact tttgcgctct tcatgagatt
      481 tagcgaaacc tcatgagaca ataaattaat tagacgttga aaaggaagtt catcacatcg
      541 ccatctttca cgatgtaatc tttaccttct gcacgcattt tgcctgcttc tttcgcgcct
      601 tgttcacctt tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg gataaagcct
      661 ttttcaaaat cagtatggat tttgcccgct gcctgcggcg cggttgctcc aaccggaatg
      721 gtccatgcac gcacttcttt caccccagcg gtgaagtaag tttgcaggtt cagcagctta
      781 taaccggcac ggatcacacg gttcagaccc ggctcttcca gcccaagctc ctgcataaac
      841 tcgtcacgtt cttcgtcgtc cagttcggca atgtctgctt caacagcagc acaaaccgga
      901 accacaacag aaccttcttt cgccgcgatt tcacgcacct ggtcaagata tgggttgttt
      961 tcaaaaccgt cttcgttgac gttggcgatg tacattgttg gtttcagcgt caggaagctc
     1021 aggtaacgaa tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag catacctgcg
     1081 ttttccagct ggggcaggca tttttccagg accgccagct cagctttcgc gtctttatcg
     1141 ccacctttgg ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc
     1201 agcgccagtt cggtgttgat aacttcaata tcgtcagccg ggttaacttt gccggaaacg
     1261 tgaatgatgt tgtcattttc aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg
     1321 atgttggtca ggaactggtt acccagacct tcgcctttcg atgcgccttt taccagaccg
     1381 gcgatatcga caaattccat ggtcgtggga agcgtacgtt gcggttttac gatttcagcc
     1441 agttgatcca ggcgaggatc aggcattggt acgacgcctg tgttcggctc aatggtgcag
     1501 aatggaaagt tggccgcttc aataccggct ttggtcagcg cgttgaacag ggtagatttc
     1561 ccgacgttgg gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata
     1621 tcttaataat caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt
     1681 atacacggca ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca
     1741 atcggttcgt tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt
     1801 cgtcgatggc ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac
     1861 caacaacttt gtttttatcg cccggatgac cgattccgat gcgtaaacgg tgaaagttag
     1921 ggttattacc caatttactg atgatgtctt tcagtccatt gtgaccacca tggccaccgc
     1981 ccaacttaaa tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt
     2041 cgtccggatt aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca
     2101 taaatgtagt cgggactaac aggcggacat cttcgcctcc aagggtgact cgcgaagtat
     2161 aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa
     2221 cgaaccaggc accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc
     2281 cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag
     2341 tttactggtt gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa
     2401 ttgcctgagt ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt
     2461 gaaataaggg ttgtaattgt gatcacgccc gcacataacc cacagggtgt tgtctatact
     2521 ttacacataa ggaagagggg tattccctgt tacaacccag aaagttccgg aggtgacata
     2581 tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa
     2641 tggtggtcgg cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt
     2701 atttcgcaca tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg
     2761 cccgtgttgg cgggcatgaa caggtgtgcg accgttactg gtgggttcgc cactatgaca
     2821 aacgttgccg ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc
     2881 agtcagtttg cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga
     2941 ttcgggaaga acgcgaggcg tcccgggatc ggttgaatgc ctgcgcgagc catagtgcgc
     3001 agtggctgga attccacgtt gcacacgcgc agttcacatc cttcgggcag acgcttcaca
     3061 aaacgctgga acgcatcaag accaccagca tcaagtaccg gaacggcatc ccacttcaga
     3121 atcacaatcc gtttgccttc aagacgtgac tccaggtccg tgaataagcc ttcagcagca
     3181 gcaaaaaaca gcgggccaat aacgcgcaga accaggacat cgtctggaac atctacgact
     3241 accggtgcca ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg
     3301 atccccacgc tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc
     3361 atgacaatga tgtcatcttt cggtgcatga cgcagcaagt cgaccacttt atgcgcttca
     3421 ctcatgttcc acgccaccat caacagcagg gctgccatag cggaaagcgg cagccaggag
     3481 agcagcggtg ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgccgag
     3541 ataggggaag ttgccccggc acggacgtta gcggcagaac gcgcgatggc agcggtagcg
     3601 gtaataccac caaagaacgg agcgatgata ttccccagcc cctgtccaac cagttcgcta
     3661 ttcgccttgt gcttcgtccc ggtcatacca tccagcacca cggcgcagag cagagattcg
     3721 attgcgccga gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag
     3781 gttagcgtga attctgaatt aggcagatcc cacggcagca ccagttgcgg tagcagttgc
     3841 ggaataccgt taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta
     3901 gcaacatgtc cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc
     3961 ggaaggtgac cgggtaaacg aatgcccaga cgcggccaga aaacaagaat acctagcgtc
     4021 acaatgccaa tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg
     4081 actttttgta gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc
     4141 tgcatggtac cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga
     4201 atatactcaa tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa gatccccgac
     4261 agcaaggtcg caaccagcag tcctgccagc ccaaactgtt gcgaaacggg atagagaatt
     4321 accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg
     4381 acaatccccg caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata
     4441 gccaacgcca tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca
     4501 cgggtaaacc gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa
     4561 ggcatcacat gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat
     4621 ctgtcaacta aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa
     4681 aaaacccgcc gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagagat
     4741 cgattcttcg ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt
     4801 acgcacgttc ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac
     4861 ttcatcaatt acagagttac gcaggttgtt cgccgcgttg ccagagaaga tcgggtgagt
     4921 cgcgtacgca aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag
     4981 cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc
     5041 gatgatatgc atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat
     5101 atcggtatcg ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg
     5161 agaaaccaca attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct
     5221 accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc
     5281 cactgtcagc acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt
     5341 gattggtaca cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat
     5401 aacagcggtg atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc
     5461 catcaggttg tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg
     5521 tacattttca ttaatttgta cgctgacttc gccatcgcta aagcgaccta cagcggcgtc
     5581 gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc
     5641 agcaaaaagc ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag
     5701 aatctgccga aaactgtgcg ggccaggcat gatcctttcc aggcggtgta ttaaagagcg
     5761 cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag
     5821 catggctctg tgcaatgggg aaagattagc gcctttcgcc acaaagccat tgagccattc
     5881 cggggcttgc tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca
     5941 ggcccctgtc ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc
     6001 aacctcgcga aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag
     6061 caacgtttct attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac
     6121 cggagtcgga atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac
     6181 cggggttagt atttcaccaa cgccttcggc aaacgcggca tgcccccgaa caaagacagg
     6241 aacatctgcg cccagcgtca gccccatttc cgccagctca tccatgctta gcccgcattg
     6301 ccagagatga tttaatgcca ccaggaccgt cgcggcattg gatgaaccac cgccgagacc
     6361 gccgcccatc ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcggaagacg
     6421 cccgctgtct gccgcagttt tcatcaacaa ccgcgctgcg cgaacgatca ggttatcttc
     6481 atgttccacg ccttcaacgg gcgttaacag acggatatcc ccatcgtcac gaagctcaat
     6541 gctgatggtg tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc
     6601 cgcacgctga ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt
     6661 ccgcattatt tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg
     6721 agttccatat tggctggcat cgcaggttgc gttttggtgt cataaccacc ataaacaacc
     6781 ttccagtttt tgccattctg gctgtaggta atttcgctca ggcggtactg gtcgtccagt
     6841 ttgtagtcgg ttgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga
     6901 attggcattc cagtcaattt gccaatcatc tcttcggcgt catcggcggt ataacgctga
     6961 cctttattgt cgactaactg cacgttaccc ggttgagcat tcagctccag ttccgtgctg
     7021 cccaatgggt tagtgagcag cagacggtag cgatcctggc cggtttgctg ccagaaaaag
     7081 cgggcgtaca ctttttgttg gtcagaaata taagcgaacg cgccgcgagt ctgatactga
     7141 ttaagattgc gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg gcttttgcca
     7201 ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa gagcagccag cggtagcagg
     7261 cggataagac gaaaatcggg caggggcata gtgatgacaa gtccttgaga tacgttgcag
     7321 ttataaccct taatgctagc gtcaccatta gtcatcgtct atgttcaagt tgtcttaatt
     7381 gtagaaatct aacgggttcg ggcaattact ccaaaagggg gcgctccctt ttattgatct
     7441 cgcgcatacg gtatgatgcg ccgagactaa ccttatcaac gttggtatta tttcccgcag
     7501 acatgaccct tttagcgctc ggtattaacc ataaaacggc acctgtatcg ctgcgagaac
     7561 gtgtatcgtt ttcgccggac aaacttgatc aggcgctgga cagtctgctc gcacagccga
     7621 tggtgcaggg cggggtggtg ctgtcgacgt gtaaccgcac tgagctttat cttagcgtcg
     7681 aagagcagga caacttgcaa gaggcgttaa tccgctggct ttgcgattat cacaatctta
     7741 atgaagaaga tctgcgtaaa agcctctact ggcatcagga taacgacgcg gttagccatt
     7801 taatgcgtgt tgccagcggc ctggattcac tggttcttgg ggagccgcag atcctcggtc
     7861 aggttaaaaa agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc gaactggaac
     7921 gcatgttcca gaaatctttc tctgtagcga aacgcgtacg cactgaaaca gatatcggtg
     7981 ccagcgctgt gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc
     8041 tctctacggt cacagtgttg ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc
     8101 atctgcgcga acataaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc
     8161 aaattctggc agatgaagtc ggcgcggaag tgattgccct gagtgatatc gacgaacgtc
     8221 tgcgcgaagc cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag
     8281 gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg
     8341 ccgttccgcg cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagcg
     8401 ttgatgatct gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc gcagcggttg
     8461 aggcggaaac tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgagcacaaa
     8521 gcgccagcga aaccattcgc gagtatcgca gccaggcaga gcaagttcgc gatgagttaa
     8581 ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc gcaagccatt atgcaggatc
     8641 tggcatggaa actgactaac cgcttgatcc atgcgccaac gaaatcactt caacaggccg
     8701 cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc
     8761 agtacatcat tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc
     8821 caaactggaa gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca
     8881 aactatcgcc gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga
     8941 tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat
     9001 gatgctcgat gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga
     9061 aaaaagcgag caactggaac agcaattaca ggttctgtta ctgccaaaag atcctgatga
     9121 cgaacgtaac gccttcctcg aagtccgagc cggaaccggc ggcgacgaag cagcgctgtt
     9181 cgcaggcgat ctgttccgta tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga
     9241 aatcatgagc gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag
     9301 cggtgatggt gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg tgcagcgtgt
     9361 tcctgctacg gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc
     9421 agaactgcct gacgcagaac tgccggacat caacccagca gatttacgca ttgatacttt
     9481 ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac
     9541 tcacttgccg accgggattg ttgttgaatg tcaggacgaa cgttcacaac ataaaaacaa
     9601 agctaaagca ctttctgtac tcggtgctcg catccacgct gctgaaatgg caaaacggca
     9661 acaggccgaa gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa
     9721 ccgtacttac aacttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta
     9781 ccgcctggat gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga
     9841 acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg
     9901 ttacgtgaag caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc
     9961 ctgctggagc atgttaccgg cagagggcgt acttttattc tcgcctttgg tgaaacgcag
    10021 ctgactgacg aacaatgtca gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa
    10081 cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca
    10141 gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg agcaggcact ggcgcggttg
    10201 cctgaacaac cttgccgtat tctcgatctc gggacgggta ccggggcgat tgcgcttgcg
    10261 ctggctcgcg agcgcccgga ctgcgaaatt accgctgtag atcgtatgcc cgatgctgtc
    10321 gccctggcac aacgtaatgc ccagcatctg gcgatcaaaa atatccacat tctgcaaagc
    10381 gactggttta gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat
    10441 attgacgagc aggaccctca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg
    10501 ctggttgcgg cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac
    10561 gcgctggtat ccggcggctt tctgcttctg gaacatggct ggcagcaggg cgaagcggtg
    10621 cgacaggcat ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat
    10681 aacgagcgcg taacgctcgg ccgctattat caatgacaag tttttctaca ctgcttagtg
    10741 ttcatcttat tagtatcgcg ctttctgttg ggctattaac cttacgtttc tggctacgtt
    10801 atcagaagca tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata
    10861 cggtgttatt gttaagcggc attgcgttga tggctaaagc gcacatcctg ccattttccg
    10921 ggcaggcaca gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt
    10981 ttattgcact cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc
    11041 tgacgttggt ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg
    11101 ggtaagtcat gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca
    11161 tgatcctggc ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac
    11221 tggagcgtct cgttagtctg gcgaaggaag aaatcagcca gcttctgcct ttagaagagc
    11281 agctggaaaa actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg
    11341 tttatcgtct ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg
    11401 cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc
    11461 cggtgatttt ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc
    11521 tgattaatcc ttttaacggt gaatcgttaa gcgaacatat gctggacgta tggttaaagg
    11581 gaaatatcag cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg
    11641 aggtaatccg caaattgctg gatacactca aagcctcgtt gatggaagaa aatcagatgg
    11701 agctggcgtt acgcaccagc gaagctttat tacaattcaa cccggaagat ccctatgaaa
    11761 ttcgcgatcg cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt
    11821 taagttattt cgttgaacag tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa
    11881 taaataacat cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaagg
    11941 ttaaggcgat cctatgaaac aaaaagtggt tagcattggc gacatcaacg tagcaaatga
    12001 cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg
    12061 catttgcgag cactacgtaa ccgtgaccca gaaactgggt atcccttacg tgttcaaagc
    12121 ctcttttgac aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga
    12181 agggatgaaa atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt
    12241 tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc
    12301 gtttcttgct cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa
    12361 cgtcaagaaa ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa
    12421 agaaggcggc aacgaaaaag tgattctttg cgatcgtggt gctaacttcg gctatgacaa
    12481 cctggttgtc gatatgctgg gcttcagcat tatgaagaaa gtgtctggta actcgccggt
    12541 gattttcgac gtgacccacg cattgcaatg ccgcgatcct tttggcgcag cttccggcgg
    12601 tcgtcgtgct caggtggctg agctggcacg agccggtatg gcggtaggtc tggcggggct
    12661 gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc
    12721 gctggctaaa ctggaaccgt tcctcaggca gatgaaagcg attgatgatc tggtgaaagg
    12781 tttcgaagaa ctggatacca gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt
    12841 tctgcgttaa gacttttttc atgggtgccg gatacaaaaa aggccgcagg ctgttaccct
    12901 gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt tcgccttact
    12961 tccggttacg ccaccagccg acaatcgctg cggtaataat tcccgccagg atcggtgctg
    13021 ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg ccttgttgta
    13081 atgacaacat tttgcggcta ttcttgaatt gttgatggtt caagattagc ccccgttctg
    13141 ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
    13201 gggataaagc ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta
    13261 cgactcaatt catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt
    13321 tttcaacagc tgttacagtc gtttcatgag tgctctggat gatgcttcca gctcgggttg
    13381 ccaatattta cttgtggaag tgataaagac aaaaatggcc gcaggctgtt acccctgcgg
    13441 ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg
    13501 gttacgccac cagccgacaa tcgctgcggt aataattccc gccaggatcg gtgccgccag
    13561 gtcgtgccag aaagtcatgg caaactgcgc gagcgtcata tagccgcctt gttgtaatga
    13621 caacattttg cggctattct tgaagtgtct ggtttcaaga ttagccctgt tgtcaggttt
    13681 tacctctcaa cgtgcggggg ttttctcttt ccagcaacca atgccaccag ggataaaacc
    13741 cccgcaacat tgcgcctcac cggataacgc cggcttggtg tggatactac gtctcaattc
    13801 atcttcactt catccctgaa atgtttgcaa taaagagtac attccggctt ttcaacagct
    13861 gttgcagtgg tttcgtgcag gttgtggtac aggctcgcaa ttctgattaa cgacctgtcc
    13921 aggtagtatg aagaaataca ataagcagga atttatatta tctcccccgg tttccgtcat
    13981 cggaggagat ataactgtca ggcaaatatc gtcatcaaat aggcggcaaa cagtgccaga
    14041 tgcgctgcgc cattgagcac gttagtacgt ccggtggaga aggagatatg gcacagcact
    14101 aaagaggcca ccatcaccac catttctggc gcaccaagtg caaactgcaa ttcgttaccc
    14161 gtcataaagg caattagcgt gacgacaggt acggtaagcg aaattgttgc taacactgaa
    14221 ccaaagaaca gattcatcgc gcgctggacc tggttgttca acactgcttt taatgcacct
    14281 aaaccttccg gcgacagaat caacagtgcc accaggaagc cagtaaaggc gacaggggca
    14341 ttcatgctgt cgagcaaagt ctccagcggg ctggcgttca ttttggtgac tgcaataacg
    14401 gcaatcaggt ggataatcaa ccagatagca tgccacaggc tgctatgggc agacggttta
    14461 ccgtggtgcg ggtcgtcgtc atcactgtca tcttcgtgct cgtagacaaa caaactttga
    14521 tgcgttttgg tctggatcag caaaaataca ccatacattg ccgcagaaat taatgctacc
    14581 agtaacgcct gaccggttga aaaattcgcc gcaggcagag ccattggaaa taccagtacg
    14641 attatcgcca gggggaacag cgcaattaaa tactgcttaa taccaaacag attcatatat
    14701 tgggtggcaa acttacgacc gcccaacaat aatgaaaagc cgaccagccc accggtaaca
    14761 atcataatga tcgaatagag cgtatcacgc attagcgttg gcgcggcgtc gccggttgcc
    14821 attaaagctg aaatcaaact aacttcaaga ataaccactg aaaggctaag aataagcgaa
    14881 ccgtaaggtt ctcccaggcg atgggctaat acgtccgcat gacggacaac actaaaggcg
    14941 ctacttaaaa taccaataag cgcaagaaga ttgatggcaa tgaccactgg tagtgtctgg
    15001 ctgcttcccc acaggaacag cactaccagc gccagaaccg ggaaaataag cgaagtctcc
    15061 ttgtggcggg tttttaccgc ctcttgagca tttgacatta tggttatccc tttgcagatg
    15121 aatttatcga aaatgtaaaa aataggtagg aaaaataaca gaaagtgtct ggatatcggt
    15181 aacattttac gaatttttac cccgttgtcg attttttact cattggggca taaaaataag
    15241 tattacgttt aggcaatgtt tgttttagcg tttcttaaga acagtctgac atgaaaattc
    15301 ttatgttttt gcaagagtag atattgttga ccacacttaa tgttcaactt tgtaaaagga
    15361 gtcaacgatg ccgtataaaa cgaaaagcga cctgccggaa agcgtaaagc acgttctacc
    15421 gtctcatgcc caggatatct ataaagaagc gttcaacagc gcatgggatc aatataaaga
    15481 taaagaagat cggcgtgatg acgccagtcg cgaagaaacc gcgcataaag tggcctgggc
    15541 tgctgtgaag catgaatatg ccaaagggga tgatgataaa tggcataaaa aatcgtaaaa
    15601 ccggtagctt agttaaagct attcgtgcgg tgttgccttg caagtggtcc gcggattgca
    15661 tattgtcccg ttagtggttt caaaatgagc agtaaaaatg tccggaagac accaaaaagt
    15721 tgtcgcaggg aagcatgcag tggcggaggt gtaagaaggt gataacgcgt gatttcttga
    15781 tgaatgccga ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag
    15841 aacaacgggc ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag agaccggtgt
    15901 ggatcttcgg ttatggatcg ttgatgtgga atccggcact ggagtttacc gagtcgtgca
    15961 ccggtacact ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga
    16021 ctgcgcacca gccgggacgg atgcttgcac taaaagaggg cggacgtacc acaggcgtcg
    16081 cctatcgact gccagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga
    16141 tgattaccgg ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag
    16201 taaacgccat tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg
    16261 ctcaggtcat cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aacgcacaat
    16321 acctgttttc actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg
    16381 atttgctggt atcggtaaaa aaactgctgg cggagaatta tccggatggt gtgttgcggc
    16441 cgggattcgc ctgagtaaac ttccctcatc gtggggcgtc agacgccccc tcaaacatta
    16501 aaatgtgagc actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat
    16561 ttccacccca tcaatcagag gaagtgtact aatcccgcgc ccgtcggtac aggttttgca
    16621 caatttcacc ggtacattct gagcggtaag gatctccagc atttgctgta tgttgtagcc
    16681 ttcccctggt ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg
    16741 cagatccaga ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa
    16801 ggattcgctc ccgtaaggtg cgccattggc aacgatcacg attttttgca ttatttactc
    16861 ctgtattcag ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa
    16921 tcggtcacat ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt
    16981 aatcgttatt ctttaaggct atgttttttc attttttacc ggaagttacc gacgttttga
    17041 gccgtttcgt tcctcgcatt attccgtttt atttactctt gctggcggca ggcggtacag
    17101 ctaacgcaca atctaccttc gagcaaaaag cggcaaatcc ctttgataat aacaatgatg
    17161 gtctgccgga tttaggcatg gcgcctgaaa atcatgatgg ggaaaaacac tttgctgaaa
    17221 ttgtgaaaga tttcggcgaa accagtatga atgataacgg gctggatact ggcgagcagg
    17281 caaaagcttt cgcattggga aaagtccgcg acgcgcttag tcaacaggtt aatcagcacg
    17341 tagagtcgtg gctatcaccg tggggaaatg ccagtgttga cgtcaaagtg gataacgaag
    17401 gtcattttac cggcagtcgt ggaagctggt ttgtgccgtt acaagataat gatcgttatc
    17461 tcacctggag ccagcttggt cttactcagc aggatgatgg gctggtgagc aatgtgggcg
    17521 ttgggcaacg ctgggcgcgc ggcaactggc tggtgggtta taacactttt tatgacaact
    17581 tgctggatga aaatcttcag cgagcgggct ttggtgccga agcgtggggc gaatatttgc
    17641 gactatcggc aaacttttat cagccgtttg ctgcatggca tgaacagaca gccacgcagg
    17701 aacaacggat ggcgcgcggg tacgacctga cagcccggat gcgcatgccg ttctatcaac
    17761 acctcaatac cagtgtcagc gtagaacagt attttggtga tcgtgttgat ttgtttaact
    17821 ctggtacggg ttatcacaat cccgtcgcgt tgagtctggg attaaattac acccctgtgc
    17881 cattagtcac tgtgacggcc cagcataaac agggtgaaag tggcgagaat caaaataacc
    17941 tcgggctgaa tcttaattac cgctttggtg taccgctcaa aaaacaactt tctgcgggcg
    18001 aggttgccga aagtcagtcg ttacgtggta gtcgctatga taatccgcag cgaaataatc
    18061 taccgactct tgagtaccga cagcgaaaaa cgttaacggt gtttcttgcg acaccgccgt
    18121 gggatctaaa acctggcgaa acagtgccgc tgaaattaca aatccgcagt cgttacggta
    18181 ttcggcaact gatttggcag ggcgatacgc agatattaag tttgacgccg ggcgcacaag
    18241 ccaacagcgc ggagggctgg acgctgatca tgcctgactg gcagaacggg gaaggggcga
    18301 gcaatcactg gcgattgtcg gtggtggtgg aagataacca ggggcagcgt gtctcctcca
    18361 atgagatcac gctaacgctt gtcgaaccgt tcgacgcatt gtcaaacgac gaactgcgct
    18421 gggaaccgta atcagaaaat gcgctcctga tgcacccata ccgctgcttc cacgcgagac
    18481 ttgagcttca ttttcttcag catgtgcttg acgtgcactt ttactgtgct ttcggtgatg
    18541 tccaggcggc gggcaatcat cttgttcggc aaaccctggg caatcagctt gagaatatcg
    18601 cgctcgcgtg gggttaactg gttaacatcg cgctcagtcg tggcacggtt agcgcgcaag
    18661 ctggcggcca gaacaggcgt taatgcttcg cttaatacca tttcgccagc agctgcctga
    18721 tgcaatgctt tcagcagatc ttccggttcc atatctttta acagatagcc atccgcgccg
    18781 cgtttcagtg cggtgaccac atcttcttca tggttagaga cgctgaatac cacaatgcgc
    18841 cctgagaggg acttttcgcg cagtttatcc agcgtttcca gaccgttcat gccgggcata
    18901 ttgagatcta acaggatcag atcgggatca agagattccg ccagttcaat accctgttcg
    18961 ccattactcg cttcgccaac cacggtgata tctggtgcca tactgataag ctgttttacg
    19021 ccagttcgca gcatcgggtg atcgtcaatc agcaggatag tagccggttc ctgattactc
    19081 atgggtatct ccttggacgt ctgtgaaagt tttttcggga ataaaggtga ccaccacttc
    19141 ggtgccacct gattcacgac ggcggacgcg gcaatcgcct cgtaaacttt gcgcacggtc
    19201 gcgcattatt atcatgccgt agtgattgct gcggatggca ttttcaggca cgccgcagcc
    19261 gttatcctgg acggtcagtt tgacctgatt atcgttttgc gccaccgtca ccacgacctc
    19321 actcgcttgc gaatgtttga gggcgttact taatgcctca cgggcaattt gcaacaagtg
    19381 gattgcctga tgcgaaggca ccagacgagg cggcaattga taatccagct tcaccgggaa
    19441 gccaaatttg gcgctgtact cttcgcaact cgcctccagc gccggacgta atccaggctc
    19501 ggtgagctgc aagcggaatg tggtgagcaa ttcacgcaac tgcgcccagg atgcattcag
    19561 ttcgttacgg atctgactta acagttcgcg gctgctttct ggcagcgcat cgccctgcat
    19621 ctgtaaacaa ctcacctgca tcttcatgca agagagagat tgggcaatag aatcatgcag
    19681 ttcgcgcgca atggtggcac gctcttccat cacgatcaac tgttgctgac gttcctgatg
    19741 ccgatccagc gccagcgtgg cggtgagttg ttcaaccagg gtatccacca gttgttgttg
    19801 atcatggcta agatgacgcc cctgcggtag ggtcgccagc aaaataccgt actgcgtatg
    19861 agagtcagcc agccgccact tcagggtcgt gccgcgatca ccaacgggta atacgccgcg
    19921 cgggcagagc tggcagcctt tatcatcaca agtcatatct ggctggcagg taaactcctg
    19981 atgattctct tcatcatcag tgtcatacac ccgcagttcg atatcacgta gcagggttaa
    20041 attctgtaag ccgttgagta caggtgacag gcgttcacac agcggggcgc gggaatgcaa
    20101 acggcggtta gcctgccata aaaaagagag gatctgattt ttatgctcca gcccggcggt
    20161 tttctcctga acccgctgct caagtacggc ataactttcg gccagttctg cagacatatt
    20221 gttcaacgca gttccaagca tcgccatttc gttgcgcccg ctgatgtttg cgcgttgggt
    20281 aaaatcgcga tggctgacgg cactcgccat tgccagcagt tgccgccacg gttgtagcag
    20341 tcgcgcccgc aaccagataa tagtgaacac cagtaaaagt gccataaata ccgccattac
    20401 ccgatggacc agtaccactg tctcaatgcg catttccgtg gtgcggtcaa aaccagatac
    20461 cagctgatca agcccggcaa caaactggct gacatccgct gacaccgttt cgcggttttg
    20521 tgcacgcatc agtgcaggga tgagttcatt acgccagtaa tcttgtaaac cctgtaattg
    20581 cgccagttgt ccgtctcgtt ctgctgctcg agtcaactcg gcgctaaatg ccgtttgttc
    20641 catctcttta attaagggct tgtctttctc gcttaatggc actgctgcca acagacggta
    20701 actttgcatg cgcagcgatc ccgctttgtt gatcgcatgg gcgctgccct gaacgccttg
    20761 caccagccag ccagaaaccg ccatccctgc cagtccaata gcagtagaaa gcaacacaat
    20821 aagcgcaacc tgattaacca gggtgagcgg agagagacaa cgtttaagca tgtaaacctc
    20881 ttccttcagg ctttaaatga gcaataacct taatgaatgt gacgatacat tctggaatgg
    20941 cagtattctc ggctattggc tgaagtatac ccatacccgg aaagagttac tccttatttg
    21001 ccgtgtggtt agtagcttta catcggtaag ggtagggatt ttacagcacc gtgaaaaatc
    21061 tcataatttt tatgaaatca ctgtactcac tatgggtaat gataaatatc aatgatagat
    21121 aaagttatct tatcgtttga tttacatcaa attgccttta gctacagaca ctaaggtggc
    21181 agacatcgaa acgagtatca gaggtgtcta tgagtcactc atccgccccc gaaagggcta
    21241 ctggagctgt cattacagat tggcgaccgg aagatcctgc gttctggcaa caacgcggtc
    21301 aacgtattgc cagccgcaac ctgtggattt ccgttccctg tctgctgctg gcgttttgcg
    21361 tatggatgtt gttcagcgct gttgcggtga acttaccgaa agtcggtttt aattttacga
    21421 ccgatcagct atttatgttg actgcgttgc cttcggtttc tggcgcgtta ttacgtgttc
    21481 catactcctt tatggttcct atcttcggtg gtcgtcgctg gacggcgttc agcaccggta
    21541 ttctgattat tccttgtgtc tggctgggtt ttgccgtgca ggatacctcc acgccttata
    21601 gcgtcttcat catcatctct ctgctgtgcg gctttgctgg cgcgaacttc gcatccagta
    21661 tggcaaacat cagcttcttc tttccgaaac agaagcaggg tggcgcgctg ggcctgaatg
    21721 gtggtctggg caacatgggc gttagcgtta tgcagttggt tgctccgctg gtggtatcac
    21781 tgtcgatttt cgcagtattt ggtagccagg gcgtcaaaca gccggatggg actgagctgt
    21841 atctggcgaa tgcgtcctgg atatgggtgc cgttccttgc catcttcacc attgcggcgt
    21901 ggtttggcat gaacgatctt gctacctcga aagcctccat caaggagcag ttgccggtac
    21961 tcaaacgggg tcatctgtgg attatgagcc tgctgtatct ggcaaccttc ggttccttca
    22021 tcggcttctc cgcgggcttt gcgatgctgt caaaaacgca gttcccggat gttcagattc
    22081 tgcaatacgc tttcttcggg ccgtttattg gtgcgctggc gcgttctgca ggtggtgcat
    22141 tatctgaccg tctgggcgga actcgtgtca cgctggtgaa ctttattctg atggcgattt
    22201 tcagcggcct gctgttcctg accttaccga ctgacgggca gggcggaagc ttcatggcgt
    22261 tcttcgcggt cttcctggcg ctgttcctga cagctgggct gggtagtggt tccactttcc
    22321 agatgatttc cgtgatcttc cgtaaactga caatggatcg cgtgaaagca gaagggggtt
    22381 ctgacgaacg tgcgatgcgt gaagcggcaa ccgacacggc ggcggcgttg gggttcatct
    22441 ctgcgattgg cgcgattggt ggcttcttta tcccgaaagc gttcggtagc tcgctggcat
    22501 taacgggttc gccagtcggc gcaatgaaag tatttttgat tttctatatc gcctgcgtag
    22561 tgattacctg ggcggtatat ggtcggcatt ctaaaaaata aatcttaata tttgattatc
    22621 ctttgcgcgg catgatgtcg cgcttttttt atgcgtcatt tagttacaac atactaatgt
    22681 tatatggttt atttcgccgg atttcattaa gaaccattaa
//
LOCUS       DAZGXS010000072        22251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGXS010000072 DAZGXS010000000
VERSION     DAZGXS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            123..791
                     /locus_tag="ACHKG5_002871"
     CDS             123..791
                     /locus_tag="ACHKG5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0868941.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPSQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            835..5601
                     /gene="ehaG"
                     /locus_tag="ACHKG5_002872"
     CDS             835..5601
                     /gene="ehaG"
                     /locus_tag="ACHKG5_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP0868942.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5969..7624
                     /gene="lldP"
                     /locus_tag="ACHKG5_002873"
     CDS             5969..7624
                     /gene="lldP"
                     /locus_tag="ACHKG5_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0868943.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7624..8400
                     /gene="lldR"
                     /locus_tag="ACHKG5_002874"
     CDS             7624..8400
                     /gene="lldR"
                     /locus_tag="ACHKG5_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0868944.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8397..9587
                     /gene="lldD"
                     /locus_tag="ACHKG5_002875"
     CDS             8397..9587
                     /gene="lldD"
                     /locus_tag="ACHKG5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0868945.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9773..10246
                     /gene="trmL"
                     /locus_tag="ACHKG5_002876"
     CDS             9773..10246
                     /gene="trmL"
                     /locus_tag="ACHKG5_002876"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0868946.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPIGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMAPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10299..11120)
                     /gene="cysE"
                     /locus_tag="ACHKG5_002877"
     CDS             complement(10299..11120)
                     /gene="cysE"
                     /locus_tag="ACHKG5_002877"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0868947.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11200..12219)
                     /gene="gpsA"
                     /locus_tag="ACHKG5_002878"
     CDS             complement(11200..12219)
                     /gene="gpsA"
                     /locus_tag="ACHKG5_002878"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0868948.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12219..12686)
                     /gene="secB"
                     /locus_tag="ACHKG5_002879"
     CDS             complement(12219..12686)
                     /gene="secB"
                     /locus_tag="ACHKG5_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0868949.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12749..13000)
                     /gene="grxC"
                     /locus_tag="ACHKG5_002880"
     CDS             complement(12749..13000)
                     /gene="grxC"
                     /locus_tag="ACHKG5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0868950.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13142..13573)
                     /gene="yibN"
                     /locus_tag="ACHKG5_002881"
     CDS             complement(13142..13573)
                     /gene="yibN"
                     /locus_tag="ACHKG5_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0868951.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13818..15362
                     /gene="gpmM"
                     /locus_tag="ACHKG5_002882"
                     /gene_synonym="pgmI"
     CDS             13818..15362
                     /gene="gpmM"
                     /locus_tag="ACHKG5_002882"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0868952.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15396..16655
                     /gene="envC"
                     /locus_tag="ACHKG5_002883"
     CDS             15396..16655
                     /gene="envC"
                     /locus_tag="ACHKG5_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0868953.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16659..17618
                     /gene="yibQ"
                     /locus_tag="ACHKG5_002884"
     CDS             16659..17618
                     /gene="yibQ"
                     /locus_tag="ACHKG5_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0868954.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17605..18640)
                     /gene="waaH"
                     /locus_tag="ACHKG5_002885"
                     /pseudo
     CDS             complement(17605..18640)
                     /gene="waaH"
                     /locus_tag="ACHKG5_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(18879..19904)
                     /gene="tdh"
                     /locus_tag="ACHKG5_002886"
     CDS             complement(18879..19904)
                     /gene="tdh"
                     /locus_tag="ACHKG5_002886"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0868955.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(19914..21110)
                     /gene="kbl"
                     /locus_tag="ACHKG5_002887"
     CDS             complement(19914..21110)
                     /gene="kbl"
                     /locus_tag="ACHKG5_002887"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0868956.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21385..22242)
                     /gene="yibB"
                     /locus_tag="ACHKG5_002888"
     CDS             complement(21385..22242)
                     /gene="yibB"
                     /locus_tag="ACHKG5_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP0868957.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5674 a         5741 c         5617 g         5219 t
ORIGIN      
        1 atcgacacac atcactgtca ttaatgtaaa gcaacataga tcaccaataa tacaatggac
       61 aactctaccg gcattgatcg ctgggcggag gaaataagga tttttacatg aactagaaga
      121 agattttttt cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca
      181 ttgaagaagg caagccagaa agtagtttat tgaaacaaca agaagaacat aacaataaaa
      241 ttgatctact tgaaaaacaa caagcacagc taaagagtca actggaaact attcaaaaac
      301 aacaaactgg cataataagc agtacaaaga ctttaactca cgtaattaaa tccgttaaag
      361 atcaacaaaa cacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc
      421 tgaacaatgg ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg
      481 gcagtgttat tcatatttca ctggtcaact tattaagtac acctatctca aatattggtt
      541 ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac
      601 aattactttt caacacatca atgaaatcca cattgaaatt attaccaagt caatggcaag
      661 acattaattt gaccctgaag ggtgtatcgc ccaataacct gggatatctg aagttagcca
      721 tcaacatgga aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg cagaaaagaa
      781 gcaaaaaata aatgcagtta gtacgcttat aacaaggaat agcgagcatc attaatgaac
      841 aaaatattta aagttatctg gaaccctgcg acagggaatt atactgttac cagcgaaacg
      901 gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt taatttctgc gctggttgcg
      961 ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa cggtcagggt
     1021 gttgattacg gtagtggatc agctggcgac ggctgggttg ctataggcaa aggggcgaaa
     1081 gcaaatactt ttatgaacac cagtggttcc agtactgctg tgggttatga cgctatagct
     1141 gaaggccaat atagctctgc catcgggtca aaaacccatg cgattggtgg tgcatcaatg
     1201 gcctttgggg ttagtgcaat atcagaaggc gatagaagta tagcactggg tgcctcttcg
     1261 tattcattgg gccaatactc aatggccctc ggccgttatt caaaagcatt gggtaaattg
     1321 tctattgcta tgggggactc ttccaaagcg gaaggagcaa acgccattgc cctgggaaat
     1381 gccactaaag ctactgagat tatgagtatt gctcttggcg acaccgccaa tgcgtcaaaa
     1441 gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc tattgcgata
     1501 ggtgctgaga ccgaagccgc tgaaaatgca actgctattg gcaataatgc gaaggcaaaa
     1561 gggactaata gcatggcaat ggggttcgga agccttgccg ataaagtcaa tactatcgca
     1621 ttaggaaatg gcagccaggc tctggcagat aatgcaatcg ccataggcca gggcaacaaa
     1681 gctgatggcg tggatgccat cgctctgggt aatggtagcc agtcgagagg cttaaacacc
     1741 attgccttag gcacagccag taatgcaact ggtgataaga gtcttgcgct tggtagtaat
     1801 agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag attccattgc ggatttagac
     1861 aataccgtct ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt gaaaaatggc
     1921 gcgatcaagt ctgacagtta cgatgccatt aatggttcac agctttatgc cattagcgac
     1981 tcggtagcaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca
     2041 gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta
     2101 cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt
     2161 actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc
     2221 agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac
     2281 accgccaata tcgctactaa taccagcaac attgccacga atacggcaaa tattgccacc
     2341 aataccacca atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg
     2401 ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa
     2461 atcaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct
     2521 cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact
     2581 tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt gactaatctt
     2641 ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc
     2701 gagaccacca gcaaaatcac caacgttaaa gatggcgacc tgacgactgg cagcaccgat
     2761 gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat
     2821 atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc
     2941 gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt
     3001 aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct
     3061 tctgtgaata ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat
     3121 tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat
     3181 gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac
     3241 gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta
     3301 aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc
     3361 gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac
     3421 gataatgtcg gtgatgccct gaatgctatc gataccactc ttgacgacgc tctgctctgg
     3481 gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc
     3541 agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac
     3601 ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa
     3661 gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac
     3721 aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga tgagactgcc
     3781 aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat catcactaat
     3841 gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat
     3901 gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc
     3961 gacgcaacct atatccaaga aaacggtgcg ggtattaact atgtgcgtac taacgacgac
     4021 ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg ctacagctat aggttataac
     4081 tctgtcgcca aaggcgatag cagcgtagct attggtcagg gcagctacag cgacgttgat
     4141 acgggtatcg ccctgggtag cagctctgtt tccagccgag tgattgccaa aggctcccgt
     4201 gacaccagca taacggaaaa tggcgttgtt attggttacg acaccacgga tggcgaactg
     4261 ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc aaatcatcaa cgtagccgat
     4321 ggttccgaag cccatgacgc cgttacggtt cgtcaattgc agaatgcgat tggtgcggtc
     4381 gcaaccacgc cgactaaata cttccacgct aattcaacgg aagaagattc actggcagtg
     4441 ggaactgact cgctggcaat gggtgcgaaa accatcgtga atggcgataa aggtattggt
     4501 atcggttatg gtgcctacgt ggacgcgaat gcacttaacg gcattgccat tggtagcaat
     4561 gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg gttctacgac cactcgtggc
     4621 gctcaaacca attataccgc ctacaacatg gacgcaccgc agaactctgt cggtgaattc
     4681 tcagtcggta gtgcggatgg tcaacgtcag atcactaacg tcgcagcagg ttcggctgat
     4741 accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg cgcaggtttc ccagaatacc
     4801 cagagcatta ctaacctgga taatcgggta acgaatcttg attcacgcgt caccaatatc
     4861 gaaaacggta ttggcgatat cgtcaccacc ggtagcacca agtacttcaa gaccaatacc
     4921 gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg cgattggttc cggctccatt
     4981 gctgccgctg acaacagcgt cgctctgggt acagggtctg tggcaaccga agaaaatacg
     5041 atctctgtag gttcctctac taaccaacgt cgtatcacca acgtagctgc aggtaaaaat
     5101 gctaccgatg ctgttaacgt ggcacagttg aagtcttccg aagctggcgg tgtacgttac
     5161 gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg cggcaacggc
     5221 ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgt ggtgaattac
     5281 gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg aatggttgag
     5341 atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg
     5401 gcaatgaccg gtctgccgca ggcttacact ccaggtgcca gcatggcctc tattggtggc
     5461 ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt
     5521 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc
     5581 ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa
     5641 ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg
     5701 caattctctg atgaggattg cccttttctt taccagacat ctccccccac aagaattggc
     5761 cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc
     5821 aatattgcca atacataaca tttagttaac cattcattgt cattatccct acacaacaca
     5881 attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac
     5941 gatgtgcgtg gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
     6001 cgggaatatc tggctttcca gtttgatagc atcgcttccc atcctgtttt tcttctttgc
     6061 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct
     6121 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta
     6181 tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta
     6241 taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac
     6301 ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
     6361 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt
     6421 taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg
     6481 cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat
     6541 tggccagatg gtggggcggc agctaccgtt tatgaccatt atcgtgctgt tctggatcat
     6601 ggcgattatg gacggctggc gcggtatcaa ggagacgtgg cctgcggtcg tggttgcggg
     6661 cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga
     6721 cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcagcc
     6781 agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag gttgatatga cgctagccca
     6841 caccggttac actgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgacagc
     6901 taccgtaaca ctgtggagta tcccgccgtt taaagccctg ttcgcatcgg gtggcgcgct
     6961 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc
     7021 gccagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc
     7081 caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc
     7141 gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc
     7201 catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact
     7261 ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
     7321 gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca
     7381 agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac
     7441 cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
     7501 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
     7561 cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc
     7621 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
     7681 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg
     7741 caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt gagcgaaggc
     7801 gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
     7861 gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc
     7921 gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc
     7981 gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
     8041 ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
     8101 aacatcgtgc tgctgcaaac catacgcggt ttcttcgatg tcctgcaatc ctcagtgaag
     8161 catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat
     8221 caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg
     8281 gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac
     8341 gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga
     8401 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
     8461 tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt
     8521 tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtctgactta agcctggaaa
     8581 cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg
     8641 gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc
     8701 cgtttactct ctcaacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc
     8761 gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg
     8821 agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg
     8881 gagcgcgtta tcgtgatgcg cattctggga tgagcggccc aaatgcggca atgcgccgct
     8941 acttgcaagc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccgc
     9001 atgatttagg taatatttcg gcttacctcg gcaaaccgac cggactggaa gattacatcg
     9061 gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
     9121 atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg
     9181 cagtacgttt tggtgctgat gggattgtag tttctaacca cggtggccgc cagctggacg
     9241 gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg
     9301 ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
     9361 gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg
     9421 cgggtgtagc taacctgcta aatctgatcg agaaagagat gaaagtggca atgacgctga
     9481 ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag
     9541 agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgagcccc
     9601 ctcaccctaa ccctctccct cagggagtgg ggaccgttcg gcgctgtatg tactccctca
     9661 ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggtga ggggaaaagg
     9721 ccgcactgct caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa
     9781 catcgtactt tacgaaccag aaattccgcc aaatactggc aatatcatcc gtctttgcgc
     9841 taataccggc tttcgtctgc atatcatcga accgatagga tttgcctggg acgataagcg
     9901 cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgactt
     9961 tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac
    10021 gcctgctcac agcgccgtaa actatcagga tggcgattat ctgatgttcg gcccggaaac
    10081 acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc
    10141 gatggcgccg gacagccgca gcatgaacct gtccaatgcg gtgtcagttg tggtgtatga
    10201 agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg
    10261 atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca
    10321 aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct
    10381 ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc
    10441 agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt
    10501 ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat
    10561 ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc
    10621 gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct
    10681 gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat
    10741 gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa
    10801 cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc
    10861 gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttcc
    10921 accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg
    10981 tagctcagtg cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc
    11041 agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt
    11101 tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg
    11161 cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtggctgc tgcgctcgtc
    11221 cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa
    11281 tacttgataa atttcctcgg ttattggcat ttctacgccg aagcgatgcg ccagttcgcg
    11341 gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg
    11401 tacatccatg ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt
    11461 acaagtaagc acaagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc
    11521 cagcgccgcg ccaagacgcg acatttcagc cagcccacgg gtgatcagtg ccgtacgcgc
    11581 attcgcacca aaaccgatac cgtcggacat ccccgcgcca atggcaatga cgtttttcac
    11641 cgcaccgcca agctgcacgc caatgaaatc cggattgctg taaacgcgga aacttttgcc
    11701 gcagtgcagc agctgctgga gatcatcggc aaaggtctga tcggtcgagg ccagcgaaat
    11761 agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag
    11821 cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc
    11881 ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg
    11941 gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac ggctggctgc
    12001 cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag
    12061 aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca
    12121 gaggacaacc tcgtggccat ttcttgccag ggtgatggca agagcggtgc cgtacgagcc
    12181 ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca
    12241 gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc
    12301 gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca ctcacgagca
    12361 tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg
    12421 atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg
    12481 cccagggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg
    12541 gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc
    12601 ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc
    12661 atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac
    12721 tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg
    12781 catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg
    12841 gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga
    12901 tcggcaactc ctgaaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat
    12961 acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt
    13021 accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac
    13081 aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa
    13141 attatttgcc gcgcaccaaa ggcaagtttt cgccagccca gccagcgacg ccttctttca
    13201 gcacgaatac ttgcgcaaaa ccagctttcg tcagtgcgtt tgcaggctcc tggcactgca
    13261 tgccagaacc gtctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat
    13321 tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga
    13381 agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag
    13441 cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa
    13501 ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca
    13561 taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga
    13621 gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg
    13681 gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg
    13741 atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg
    13801 agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg
    13861 gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa
    13921 tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg
    13981 gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc
    14041 gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg
    14101 ctaatccagt gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca
    14161 ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg
    14221 tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc
    14281 gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg
    14341 cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata
    14401 accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagttcc
    14461 aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt
    14521 tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg
    14581 atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg cgaaatcact cgtgcattcg
    14641 taaacgcaga tttcgacggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc
    14701 tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta
    14761 acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg
    14821 aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg
    14881 aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga
    14941 gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca
    15001 ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg
    15061 ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg
    15121 tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg
    15181 caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg
    15241 ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt
    15301 cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat
    15361 aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca
    15421 ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg
    15481 ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg
    15541 caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga
    15601 aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc
    15661 tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc
    15721 aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg
    15781 agcataccgg tattcagctg attctcagcg gtgaagaaag ccagcgtgga cagcgtttac
    15841 aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa
    15901 cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga gaaacagagc gagcaacaaa
    15961 cgctgttata tgagcagcgc gcccaacagg cgaaactgac tcaggcgctg aacgagcgta
    16021 aaaagacgct ggcagggctg gagtcttcca tccagcaagg tcagcaacag ttgagcgagc
    16081 tgcgcgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag
    16141 cgcgtgcaga acgagaagca cgtgaggccc aggcggttcg cgaccgccag aaagaagcga
    16201 cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg
    16261 gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc
    16321 gctatggtga acagctacag ggcgaactac gctggaaagg aatggttatc ggtgcctctg
    16381 aaggtactga agttaaagcg attgccgatg gtcgggtgat tctggctgac tggctgcaag
    16441 gttacggtct ggtggtggtg gttgagcacg gtaaaggcga catgagtctt tacggctata
    16501 atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg
    16561 tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccaaggtc
    16621 aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg
    16681 ttcttgcatt tgccgctctg ttggcgctct cctcccccgt acttgctggc aaacttgcca
    16741 tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgccct
    16801 ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag
    16861 cgcataacag cgggcacgaa gtgttgattc atctcccgat ggcaccactg agtaaacagc
    16921 cgctggagaa aaatacgctg cgcccggaga tgagcagcga cgaaattgag cgcattattc
    16981 gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg ggtagcaaga
    17041 tgacctctaa cctgtttggt atgcagaaag tgatgcaggc actggagcgt tacaatcttt
    17101 acttcctcga cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggtactg
    17161 gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc
    17221 gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg
    17281 ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag
    17341 acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acttctacac
    17401 cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca
    17461 aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa
    17521 gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac
    17581 agcccgaagc ggcgaagttt aacgctagcg caaattaaag gcgctacgcg taatgcgatg
    17641 gtgcgaccgc gtttttttcg cgccattgcc ataagcggaa agaccacagt aacgcctggt
    17701 aaccgacctt cacgctgcgc acattggtaa tcaggcgctt atacataccg gaagtaaata
    17761 tctcggcaat catccgttgg cgggtaagaa tatccggctc tttgcgcacc gcatggcaaa
    17821 cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg ataaatcata attttgtcgg
    17881 cataatttcg atttaatttc tccagcaggc gggtaatctt aatatagtga cgttgataat
    17941 taaggttttt attcccttgt ctatgcaacc gactcactga cgtattatgc agataatatt
    18001 tatataatga ttgctcggta tatcgcgcac gcagcgcgtt aaacatgaat tctgttgtcc
    18061 agacaatatc ctgatgatgt aatccggcaa taaatttaat gttattttta acaataacat
    18121 cacggcgata aacccccatc cagacaacgt gagtccaacg gcgcgaagaa agccccatcc
    18181 gcagccagtc cgggccggtt aatacaccgg ttgagcgaag gcgatcgctg gggatggatt
    18241 gccaggtttc tcccgtttca cgaaaagacc agtcagcgtt gcactgcgcc acgtcgaggt
    18301 cgtcctctaa cgccatggtc atcagcgttt cgtacatggt gggatagacc tcatcgtcag
    18361 catcgacaaa agcgacatat ttgcccgtcg ccacctcgat cccacgatta cgcgctaccg
    18421 atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg atagttttct gcgtaatgct
    18481 ttgctatttc aacagaatta tccgttgaac catcgttaat aataatgatt tccagagcag
    18541 tccaggtttg cgtaattaaa gattccatac aagtgcggaa atcatcgccc gcattatata
    18601 acggaataat aacactaagt ttattggtgc tgttcatcat aaattaccga cgacctctga
    18661 agttatcctg acgttttaca ggctttgtat taagaaacta ttaagcctgt gagatattat
    18721 ttttacaggt gagagagtca cttcagggta gagagataat gagaacagct atataaaaga
    18781 tcagcgcaat tacgaactct ttattacact gacaggattt tacatattta acagattatc
    18841 ctcaggcaaa agggctggaa taccagccct ttttcgtgtt aatcccagct cagaataact
    18901 ttcccggact ggcccgaaca catagcgtca aagcccttct ggaaatcatc gatagagaaa
    18961 cgatgggtaa tgatcggcga gagatcgagg ccagactgaa tcagcgccgc catcttgtac
    19021 caggtttcaa acatctcacg accgtaaatg cctttaatga acaagccttt aaagatcact
    19081 ttggtccagt cgatagacat gtcagacggt ggaataccca gcatcgcaat acgaccgccg
    19141 tggttcatgg tatcgagcat ggtacgaaac gctggcggcg cgccggacat ttccagaccg
    19201 acatcaaagc cttcggtcat gcccagttca gccatcacat cattaaggtt ttctttcgcg
    19261 acgttaaccg cacgggtgat acccattttg cgcgccagct caaggcggta ttcgttaaca
    19321 tcggtgatca ccacattgcg tgcaccaacg tgtttcgcca ccgccgcagc cataatacct
    19381 atcggacctg caccagaaac cagcacatct tcgcccacca gatcgaacga cagcgcggta
    19441 tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt cgtcggagat attgtcgggg
    19501 attttgaaag cgttgaacgc cggaatcacc agatattcgg cgaagcagcc cggacggtta
    19561 acaccgacac caatggtgtt gcggcacaga tgggtacgac cgccacgaca gttgcgacag
    19621 tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc cgattttgaa gcctttcact
    19681 tcctgaccaa tacctaccac ttcaccgaca tattcatggc ctacgaccat cggcaccggg
    19741 atggtttttt gcgaccactc atcccagtta tagatgtgaa cgtcagtccc gcagatggct
    19801 gttttacgga ttttaatcag cagatcgtta tgcccgagtt ccggtacagg aacgtcggtc
    19861 atccagatgc cctcttccgc tttcagtttg gataacgctt tcatctcaca tcctcaggcg
    19921 ataacgccca gttgtttacc aatacgcgtg aatgcttcta ctgcacgcgt aatttgctca
    19981 ggggtatgcg ccgcagacat ctgggtacga atacgcgcct gacctttcgg aacgaccgga
    20041 tagaagaaac cggtgacgta aatgccctct ttttgcagct cacgggcaaa tttctgcgcc
    20101 actactgcat cgccaagcat gaccggaata atggcatgat cggctcccgc cagggtaaag
    20161 cccgccgccg acatttgctc acggaactgg cgcgcgttcg cccacagacg gtcacgcagt
    20221 tcgctgcccg cttcgaccat ctccagtact ttgatggacg cggcaacaat ggccggtgcc
    20281 agcgagttgg agaacaggta cggacgagaa cgctggcgca gccactcaac cacctctttg
    20341 cgcgccgcgg tataaccacc agaagccccg cccagcgctt taccaagcgt accggtgata
    20401 atatcgaccc ggcccatcac atcgcagtat tcatgggaac cacgaccatt ttcaccgaca
    20461 aaaccgaccg cgtgggagtc atccaccatc accagggcat cgtatttatc tgccagatcg
    20521 caaacgcctt tcaggttggc aatcacgccg tccattgaga acacaccatc ggtggcaatc
    20581 agcacatgac gcgcaccggc ttcacgcgct tctttcagac gtgcttccag ctcctgcata
    20641 tcgttgttgg catagcgata gcgtttagct ttgcacagac gcacaccatc aataatagac
    20701 gcgtggttca gtgcgtcgga gataatggcg tcttccgcac ccagcagcgt ttcaaacagt
    20761 ccaccgttag catcaaagca ggaagagtag agaatcgcat cttccatccc caggaatgcc
    20821 gccagttttt gttcaagctc tttatggctg tcctgagtgc cgcaaataaa acgcaccgaa
    20881 gccatgccga aaccgtgaga atccattccc gcctttgctg ccgcaatcag atccggatga
    20941 ttcgccagcc cgagatagtt gttggcacaa aagttaatga cgtggcttcc atcagccaca
    21001 gtgatatctg cttgctgcgc agacgtaata atgcgctctt ctttaaacaa cccttccgcc
    21061 cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt ctccacgcat tgcgattctc
    21121 cagacagggc aaattccagc acatattacc caaacttata ggtcgcgacg agatgacgcg
    21181 ttaactcttc tgcaaaattc aggataaatg acgtgtaact tgatgtctct caacacctac
    21241 agatagtaga aaaactgtac agttttggct gggcataatg atgattgatt ttactgcatt
    21301 gatataaaaa gaaaaaatga tgccaaagca tcatttttcc tatctcagtt ggtatcaaaa
    21361 cgctacccac taatggaggg aaacctattt tcttgaaagg aaaattctca gcgcctgcat
    21421 ttttgcccct aaagtgttac tcctgaagca gcgaaacaaa tcgaaccatt ttcctctccc
    21481 cagatagttt aggttgaaga gatcggggcg tttgtaatag cacatgacaa aaatgccctg
    21541 gtcatcatcc acaatgttat tatttagtgt tattttttga ctttccagaa ctaatttata
    21601 gaattctttc catttatgct gtgaaccaac aattgcgccc ccaataatat atacatgatt
    21661 gccaatcatg aaatcaaaaa cctgttgttg agatgtgaca gtaagccctt tttttatagt
    21721 gaaaagatgc atcttacttt catcaaatgg gaaatcccaa attttcaatc ctcgagttac
    21781 atttggctta cggcaatagc caaaatctat ccacgcaaca agaggcgttt tcactaagcc
    21841 catgttgata gctttattta caaaataggc cttaaggtta cataccaaaa catactctgg
    21901 tgaccagtac tctgggtttt ttaattgtcg aggctcaagt ctatttgtaa acgactcatc
    21961 tttttgaatt ttttcgatac gacttctgat atatctaaat ttctttttta tatcgataac
    22021 gataaccgtt gttggttttc cgttgcgaat cgcttcaacc cttggtttca aatcgggaga
    22081 agtgaaaata atcatctcat tttcaagtgc ggccagacgc tcaaagtaac taaaataaac
    22141 atcaactgaa cgagctagtt tttcacggaa ccctttatta gcagtccaat cccctctacc
    22201 aatgtcaaaa tatgcagtga taattgtggt ggatgatttc attactaata a
//
LOCUS       DAZGXS010000073        22235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGXS010000073 DAZGXS010000000
VERSION     DAZGXS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            19..573
                     /gene="ybeT"
                     /locus_tag="ACHKG5_002889"
     CDS             19..573
                     /gene="ybeT"
                     /locus_tag="ACHKG5_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0868958.1"
                     /translation="MNKKLMYIFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /gene="djlB"
                     /locus_tag="ACHKG5_002890"
     CDS             complement(583..2010)
                     /gene="djlB"
                     /locus_tag="ACHKG5_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP0868959.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /gene="ybeR"
                     /locus_tag="ACHKG5_002891"
     CDS             complement(2007..2714)
                     /gene="ybeR"
                     /locus_tag="ACHKG5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0868960.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2879..3856
                     /gene="ybeQ"
                     /locus_tag="ACHKG5_002892"
                     /pseudo
     CDS             2879..3856
                     /gene="ybeQ"
                     /locus_tag="ACHKG5_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3926..4408)
                     /gene="ybeL"
                     /locus_tag="ACHKG5_002893"
     CDS             complement(3926..4408)
                     /gene="ybeL"
                     /locus_tag="ACHKG5_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0868961.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4644..7226
                     /gene="leuS"
                     /locus_tag="ACHKG5_002894"
     CDS             4644..7226
                     /gene="leuS"
                     /locus_tag="ACHKG5_002894"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0868962.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7241..7822
                     /gene="lptE"
                     /locus_tag="ACHKG5_002895"
     CDS             7241..7822
                     /gene="lptE"
                     /locus_tag="ACHKG5_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0868963.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7822..8853
                     /gene="holA"
                     /locus_tag="ACHKG5_002896"
     CDS             7822..8853
                     /gene="holA"
                     /locus_tag="ACHKG5_002896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0868964.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8855..9496
                     /gene="nadD"
                     /locus_tag="ACHKG5_002897"
     CDS             8855..9496
                     /gene="nadD"
                     /locus_tag="ACHKG5_002897"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0868965.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            9520..10131
                     /gene="cobC"
                     /locus_tag="ACHKG5_002898"
     CDS             9520..10131
                     /gene="cobC"
                     /locus_tag="ACHKG5_002898"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0868966.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10391..10708
                     /gene="rsfS"
                     /locus_tag="ACHKG5_002899"
     CDS             10391..10708
                     /gene="rsfS"
                     /locus_tag="ACHKG5_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0868967.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10712..11179
                     /gene="rlmH"
                     /locus_tag="ACHKG5_002900"
     CDS             10712..11179
                     /gene="rlmH"
                     /locus_tag="ACHKG5_002900"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0868968.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11210..13111
                     /gene="mrdA"
                     /locus_tag="ACHKG5_002901"
     CDS             11210..13111
                     /gene="mrdA"
                     /locus_tag="ACHKG5_002901"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0868969.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13114..14226
                     /gene="mrdB"
                     /locus_tag="ACHKG5_002902"
                     /gene_synonym="rodA"
     CDS             13114..14226
                     /gene="mrdB"
                     /locus_tag="ACHKG5_002902"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0868970.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14237..15325
                     /gene="rlpA"
                     /locus_tag="ACHKG5_002903"
     CDS             14237..15325
                     /gene="rlpA"
                     /locus_tag="ACHKG5_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0868971.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15465..16676
                     /gene="dacA"
                     /locus_tag="ACHKG5_002904"
     CDS             15465..16676
                     /gene="dacA"
                     /locus_tag="ACHKG5_002904"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0868972.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16786..17049
                     /gene="ybeD"
                     /locus_tag="ACHKG5_002905"
     CDS             16786..17049
                     /gene="ybeD"
                     /locus_tag="ACHKG5_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0868973.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17150..17791
                     /gene="lipB"
                     /locus_tag="ACHKG5_002906"
     CDS             17150..17791
                     /gene="lipB"
                     /locus_tag="ACHKG5_002906"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0868974.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18051..19004
                     /gene="ybeF"
                     /locus_tag="ACHKG5_002907"
     CDS             18051..19004
                     /gene="ybeF"
                     /locus_tag="ACHKG5_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP0868975.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19213..20178
                     /gene="lipA"
                     /locus_tag="ACHKG5_002908"
     CDS             19213..20178
                     /gene="lipA"
                     /locus_tag="ACHKG5_002908"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0868976.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20279..20482)
                     /gene="tatE"
                     /locus_tag="ACHKG5_002909"
     CDS             complement(20279..20482)
                     /gene="tatE"
                     /locus_tag="ACHKG5_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0868977.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20611..21399)
                     /locus_tag="ACHKG5_002910"
     CDS             complement(20611..21399)
                     /locus_tag="ACHKG5_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0868978.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21492..21875
                     /gene="crcB"
                     /locus_tag="ACHKG5_002911"
     CDS             21492..21875
                     /gene="crcB"
                     /locus_tag="ACHKG5_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0868979.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21929..22138)
                     /gene="cspE"
                     /locus_tag="ACHKG5_002912"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21929..22138)
                     /gene="cspE"
                     /locus_tag="ACHKG5_002912"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0868980.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         5680 a         5578 c         5526 g         5451 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcacaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcggaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccgggct tcccgcacat taacgtcgac tgtcacccga acagatggag
      601 tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag
      661 acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt
      721 ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga ttttctgcat caagacggct tacgatcggc attaatgatt cttctgtagg
      841 ttggctggcc tgcactaatt gtttggcaac tagatataat ggccataagg tatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttaca tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccaga gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctgg tcgttaacca gttcacgagc
     1081 ttcaatcatc atcaccacta atgggtaata atcgactcga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctgag tggagggata ctcgtgccag tagtcacaaa gttctgccag
     1201 caggctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag
     1321 gcgacctgca ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 ggcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1501 gatctggaag ttaaaaacat ttcccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggctatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcattcatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcactat cagaggcgag taacgcacga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 gagcgcttcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacatcag tcgtttcctc
     1981 tatgttgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     2041 catgtggggt agtcagccag tggttgccag ggtaaggtcg cgtaatatgc atcgtcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcacgtaata aaggcagatt atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2281 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2341 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca
     2461 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgcgcggca
     2521 tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2581 caggaggaag aaaggtcaaa aaagcgtgta cctaaataca ttttgggtga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gactccatgt ccatatgatt tatccttaaa tattcattga atgaatatta gccgcattgc
     2761 gacgctcgtt tatttatact tttctgacgc gacataatac gcataaaata aggcttgcgc
     2821 aaaaaatgaa acacataaaa attcaatata ttttatctac aatcataagg aaatgataat
     2881 gattttcacg tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga
     2941 aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat
     3001 tgactctccg gacgacgaaa aggcctttta ctagctgaag ctggccgctg agcaaggtca
     3061 ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga
     3121 taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc
     3181 caacgccctt ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttgc
     3241 ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc ttgggtggat
     3301 gtacagaaat ggcaacggag ccgcacaaga ctatacgctg gcatttttct ggtacaaaca
     3361 agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt atgaagacgg
     3421 aaaaggcgtt gctcaaaacg agacactcgc cgcattctgg tatttgaaaa gcgcacagca
     3481 gggtaatcgg catgctcagt ttcaaattgc atgggattat aacgctggcg aaggggtgga
     3541 ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg ggagcgtcgg
     3601 cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca
     3661 ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa
     3721 cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga
     3781 cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac
     3841 tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc ccatattctc
     3901 tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat
     3961 gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac
     4021 atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat
     4081 gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg atgtctgcca
     4141 gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca
     4201 ggctctcttc atagctcatg gcgaactctt ccaagtcacg tctgacagct cgcgtcagct
     4261 catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca
     4321 ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac gcaaccagtt
     4381 cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata
     4441 ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg
     4501 tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac
     4561 gtttgggcta tgctatgcgg atctgaaaaa ccacctctaa cgctactttt gtagccgtaa
     4621 tgaaaacagg accactggct gccatgcaag agcaataccg cccggaagag atagaatcca
     4681 aagtacagct tcattgggat gagaagcgca catttgaagt aaccgaagac gagagcaaag
     4741 agaagtatta ctgcctgtct atgcttccct atccttctgg tcgactacac atgggccacg
     4801 tacgtaacta caccatcggt gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg
     4861 ttctgcagcc gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa
     4921 acaacaccgc tccggcaccg tggacgtacg acaacatcgc gtatatgaaa aaccagctca
     4981 aaatgctggg ctttggttat gactggagcc gcgagctggc aacctgtacg ccggaatact
     5041 accgttggga acagaaattc ttcaccgagc tgtataaaaa aggcctggta tataagaaga
     5101 cttctgcggt caactggtgc ccgaacgacc agaccgtact ggcgaacgaa caagttatcg
     5161 acggctgctg ctggcgctgc gataccaaag ttgaacgtaa agagatcccg cagtggttta
     5221 tcaaaatcac tgcttacgct gacgagctgc tcaacgattt ggataaactg gatcactggc
     5281 cagacaccgt taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca
     5341 ccttcaacgt taacgactat gacaacacgc tgaccgttta cactacccgc ccggacacct
     5401 ttatgggttg tacctacctg gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg
     5461 aaaataatcc tgaactggcg gcctttattg acgaatgccg taacaccaaa gttgctgaag
     5521 ctgaaatggc gacgatggag aaaaaaggcg tcaatactgg ctttaaagct gttcacccat
     5581 taacgggcga agaaattccc gtttgggcag caaacttcgt attgatggag tacggcacgg
     5641 gcgcagtaat ggcggttccg gggcacgacc aacgcgacta cgagtttgcc tctaaatacg
     5701 gcctgaacat caaaccggtt atcctggcgg ctgacggctc tgagccagat ctttctcagc
     5761 aagccctgac tgaaaaaggc gtgctgttca actctggcga gttcaacggt cttgaccatg
     5821 aagcggcctt caacgccatc gccgataaac tgactgcgat gggcgttggc gagcgtaaag
     5881 tgaactaccg cctgcgtgac tggggtgttt ctcgtcagcg ttactggggc gcgccgattc
     5941 cgatggtgac gctggaagac ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga
     6001 tcctgccgga agatgtggtg atggacggca ttaccagccc gattaaagca gatccacagt
     6061 gggcgaaaac taccgttaac ggtatgccag cgctgcgtga aaccgacact ttcgacacct
     6121 ttatggagtc ctcctggtac tatgcgcgct acacttgccc agagtacaaa gaaggtatgc
     6181 tggattccga agcggctaac tactggctgc cggtggatat ctacattggt ggtatcgaac
     6241 acgccattat gcacctgctc tacttccgct tcttccacaa actgatgcgt gatgcaggca
     6301 tggtgaactc tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg
     6361 ccttctacta tgttggcgaa aacggcgaac gtaactgggt ttccccggtt gatgctatcg
     6421 ttgaacgtga cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg
     6481 tttataccgg catgagcaaa atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga
     6541 tggttgaacg ttacggcgcg gacaccgttc gtctgtttat gatgtttgct tctccggctg
     6601 atatgactct cgaatggcag gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg
     6661 tctggaaact ggtgtacgag catactgcga aaggtgatgt tgcggcactg aacgttgatg
     6721 cactgactga agatcagaaa gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga
     6781 ccgatgatat cggccgtcgt cagaccttca acaccgcaat tgcggcgatt atggagctga
     6841 tgaacaaact ggcgaaagca ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag
     6901 cactgctggc cgttgtccgt atgcttaacc cgttcacccc gcacatctgc ttcacgctgt
     6961 ggcaggaact gaaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa
     7021 aagcgatggt ggaagactcc acgctggtcg tggtgcaggt taacggtaaa gtccgtgcta
     7081 aaatcaccgt tccggtggac gcaacggaag aacaggttcg cgaacgtgct ggccaggaac
     7141 atctggtagc aaaatatctt gatggcgtta ctgtacgtaa agtgatttac gtaccaggta
     7201 aactcctcaa tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt
     7261 gttgttatct ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc gtgacaccac
     7321 gcaggttcct tccactatga aggtcatgat cctggactca ggcgatccga acgggccatt
     7381 aagccgtgcg gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg ataaagaaac
     7441 cacgcgtaag gacgttccat ccctgcgttt gggtgctgta tcaatctcac aggataccgc
     7501 gtcggttttc cgtaatggtc aaactgcgga atatcagatg gtgatgactg ttagcgcgtc
     7561 ggtattgatt cctggacgag atatctaccc gattagcacc aaagtcttcc gttcattctt
     7621 cgataacccg caaatggcgt tagcgaaaga taacgagcag gaaatgatca ttaaagagat
     7681 gtacgaccgt gctgccgaac agctgattcg taagctgcca agcatccgtg ctgcggatat
     7741 tcgttccgac gaagaacaga cgtcgactac aacggatact ccggcaacgc ctgcacgcgt
     7801 ctccaccacg ctgggtaact gatgattcgg ttgtacccgg aacaactccg cgcgcagctc
     7861 aatgaagggc tgcgcgcggc gtatctttta cttggtaacg atcctctgtt attgcaggaa
     7921 agccaggacg ctgttcgtca ggtagctgcg gcacaaggat tcgaagaaca ccacactttt
     7981 tccattgatc ccaacactga ctggaatgcg atcttttcgt tatgccaggc catgagtctg
     8041 tttgccagtc gacaaacgct attgctgttg ttaccagaaa acggaccgaa cgcggcgatc
     8101 aatgaacaac ttctcacact caccggactt ctgcatgacg acctgctgtt gatcgtccgc
     8161 ggtaataaat taagcaaagc gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc
     8221 agcgtgcagg tgacctgtca gacaccggag caggctcagc ttccccgctg ggttgctgcg
     8281 cgcgcaaaac agctcaactt agaactggat gacgcggcga atcaggtgct ctgctactgt
     8341 tatgaaggta acctgctggc gctggctcag gcactggagc gtttatcgct gctctggcca
     8401 gacggcaaac tgacattacc gcgcgttgaa caggcggtga atgatgccgc gcatttcact
     8461 ccttttcatt gggttgatgc tttgttgatg ggaaaaagta agcgcgcatt gcatattctt
     8521 cagcaactgc gtctggaagg cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa
     8581 ctcttgttac tggttaacct gaaacgccag tctgcccata cgccactgcg tgcgttgttt
     8641 gataagcatc gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta
     8701 agtcagccgc agttacgtca ggccgtgcaa ctcctgacac gaacggaact caccctcaaa
     8761 caagattaca gtcagtcagt gtgggcagag ttggaagggt tatctcttct gttgtgccat
     8821 aaacccctgg cggacgtatt tatcgacggt tgatatgaaa tctttacagg ctctgtttgg
     8881 cggcaccttt gatccggtgc actatggtca tctaaaaccc gtggaaacgc tggcgaattt
     8941 gattggtctg acgcgagtca caatcatccc taataatgtt cctccgcatc gtccccagcc
     9001 ggaagcgaac agcgtacagc gtaaacacat gcttgaactg gcgattgccg acaagccatt
     9061 atttactctt gatgaacgcg agctaaagcg caatgccccc tcttacactg cgcaaacact
     9121 gaaagagtgg cggcaggaac aagggccgga cgtgccgctg gcgtttatta ttggtcagga
     9181 ttcattgctg acctttccga cctggtacga atacgaaacg atactcgaca atgcacattt
     9241 gatcgtctgt cggcgtccag gttacccact tgaaatggcg caaccgcaat accagcaatg
     9301 gctggaagat catttgacac ataacccgga agatcttcac cttcagcctg ccggtaaaat
     9361 ttatctggct gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg aacgtttgca
     9421 aaacggtgaa tcatgtgatg atttattgcc ggaaccggta ctgacttaca ttaaccaaca
     9481 aggcttgtat cgctgatacc tgtcgttacg gagggcatga tgcgactgtg gttaattcgt
     9541 catggcgaaa cacaagcgaa tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg
     9601 accgcacgcg gtattgagca agcgcaaaat ctgcatacac tgctagatga tgtttccttt
     9661 gacctggttt tatgcagtga actggaacgg gcacagcata ccgcgcgact ggttctcagt
     9721 gaccgccagc tccccgttca tatcatccct gaactcaacg aaatgttttt tggcgactgg
     9781 gagatgcgac atcatcgcga cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc
     9841 aatgactggc agcatgctat ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt
     9901 gtggaacgct ttatcgcaag gcttagtgaa tatcagcact atcagaatat tttaatcgtc
     9961 agccatcagg gcgtacttag tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct
    10021 atgtggcatt ttcgcgttga ccaggggtgt tggagcgcca ttgatataaa ccaaaaattc
    10081 gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg acttttctgt
    10141 ttttttacgg gtaagccgca acgaccattg acagccccgg gcaggctgat attctccgca
    10201 gccagacttt ttccaccaga cacgactttg tagaaattgt tttacaaaaa tggcgatgca
    10261 atctgcggcg cggggtggga tgatagccca ctttcgaaag ccgattcggc gacaattgtc
    10321 ccgaaatcgc ctctggttca ggtatactga cagaccattt ttatctattt gattcaccca
    10381 gggggaaaac ttgcagggta aagcactcca ggattttgtt atcgacaaaa ttgatgacct
    10441 caaaggtcag gacatcatcg ccttagacgt tcagggcaaa tccagcatca ctgactgcat
    10501 gatcatctgt acgggtacgt ccagccgtca tgttatgtcc attgctgacc acgttgtgca
    10561 ggagtctcgc gcagcgggcc tgttaccgct cggagtagaa ggtgaaaaca gcgccgactg
    10621 gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg caggaagaga gccgtcgcct
    10681 gtatgaactg gaaaaactct ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac
    10741 gaaaatgccg gactgggtac aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga
    10801 tatgcccttc gagctgattg aaattccggc cggaaaacgc ggcaagaatg cggacatcaa
    10861 gcgcatactc gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa accgcattgt
    10921 caccctcgat attccaggca agccctggga tacgccgcag ttagccgctg agctggaacg
    10981 ctggaagctg gatggtcgcg acgtcagtct actgattggc gggcctgaag ggttgtcgcc
    11041 tgcctgtaaa gcggcggcgg agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc
    11101 gctggttcgc gtgctggtcg cagagagtct gtaccgggcg tggagcatca ccaccaacca
    11161 tccttatcac cgtgagtgat aagggagctt tgagtagaaa acgcagcgga tgaaactaca
    11221 gaactctttt cgcgactata cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc
    11281 ctttttgggg attttgctgc tgaccggcgt gcttatcgcc aacctgtata atctgcaaat
    11341 tgttcgcttt accgactacc agacccgctc taatgaaaac cgcattaagc tggtgcctat
    11401 cgcgcccagc cgcggcatta tctacgaccg taacggtatc cctctggccc tcaaccgcac
    11461 tatctaccag atagaaatga tgccggagaa agtcgataac gtgcagcaaa cgctggacgc
    11521 tttgcgcagc gtggttgatc tgaccgatga cgatattgct gcattccgaa aagagcgcgc
    11581 acgttcacac cgtttcacct ccattccggt gaaaactaac ctgaccgaag tacaagtagc
    11641 tcgctttgcc gtcaatcagt accgttttcc gggtgttgaa gttaaaggct ataaacgtcg
    11701 ttactatcct tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga
    11761 taaagacgtc gaacgcctga ataatgacgg caaactggcc aactatgcgg caacgcatga
    11821 tatcggtaag ctgggcattg agcgttacta tgaagacgtg ctgcacggtc agaccggtta
    11881 tgaagaggtt gaagttaaca accgtgggcg tgttatccgc cagttaaaag aagtcccacc
    11941 gcaagccgga cacgatattt acctgacgct ggatctcaaa ctccagcaat atattgaaac
    12001 gctgctggcg ggtagccgcg cagctgtggt agtcaccgat ccgcgtacag gtggggtgct
    12061 ggcgctggtt tccacgccta gttatgaccc aaacttgttt gttgacggta tctccagcaa
    12121 agattattcc gccttgttga acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg
    12181 ggtttatcct cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg
    12241 ggtgatcacg cgcaatacga cgctgtttga ccctggctgg tggcaactgc caggttcgga
    12301 aaaacgttat cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct
    12361 ggaagaatct gcggatacct tcttctatca ggtggcctac gatatgggga tcgatcgcct
    12421 ctccgaatgg atgggtaaat tcggttatgg tcattatacc ggtatcgacc tggcggaaga
    12481 acgttccggc aatatgccta cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta
    12541 tcagggtgac accattccgg ttggtatcgg tcagggttac tggacagcga ccccaatcca
    12601 gatgagtaag gcactgatga tcctgattaa tgacggtatc gtgaaggttc cgcatttgct
    12661 gatgagcacc gccgaagacg gcaaacaggt gccatgggta caaccgcatg aaccgcccgt
    12721 tggcgatatt cattccggtt actgggagct ggcgaaagat ggtatgtacg gtgttgctaa
    12781 ccgccctaac ggtacggcgc ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa
    12841 atctggtacc gctcaggtct tcggtctgaa agcgaacgaa acctataatg cgcacaaaat
    12901 tgccgagcgt ttacgtgacc acaaactgat gaccgccttt gcgccataca acaatccgca
    12961 agtggctgtc gccatgattc tggagaacgg tggcgcgggt ccggcggttg gtacgctgat
    13021 gcgccagatc ctcgaccaca ttatgctggg tgataacaac accgatctgc ctgcggaaaa
    13081 tcctgcggtt gccgcagcgg aggaccatta atcatgacgg ataatccgaa taaaaaaaca
    13141 ttctgggata aagtccatct cgatcccaca atgctgctga ttttactggc attgctggtt
    13201 tacagcgccc tggttatctg gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa
    13261 atcggccaaa tcgcgatggg tctggtcatc atggtggtga tggcgcaaat tcctccacgc
    13321 gtttatgaag gctgggcccc ctatctctat atcatctgta ttattttgct ggtggcggta
    13381 gatgctttcg gtgccatctc taaaggcgct caacgctggc tggacctcgg tattgttcgt
    13441 tttcagccgt cggaaattgc caaaatagcc gtaccactga tggttgcgcg ctttatcaac
    13501 cgcgacgttt gcccgccatc gttgaagaac actggcatcg cgctggtgct gatatttatg
    13561 cccacgctgc tggtggctgc acagcctgac ctgggaacat caatcctcgt tgcgctttcc
    13621 ggtctgtttg tactgttcct ctctggcctt agctggcgtc tgattggcgt cgcagtagtg
    13681 ctggtagcgg cgttcattcc gattctgtgg ttcttcctga tgcatgatta ccagcgccag
    13741 cgcgtaatga tgctcctgga cccggaatca gacccactcg gcgcgggcta tcacattatt
    13801 cagtctaaaa ttgctattgg ctccggcgga ttacgcggca aaggctggct gcacggcact
    13861 cagtcacagc ttgaatttct ccccgaacgc catactgact ttatcttcgc ggtactggcg
    13921 gaagagctgg gattagtggg cattttgatt ctgctcgctc tctacattct gctgatcatg
    13981 cgcgggctgt ggatagccgc cagagcgcaa accacctttg gtcgcgtcat ggctggcggc
    14041 ttaatgctga tattattcgt ttatgtcttc gtaaatattg gtatggtaag cggtattctg
    14101 ccggttgtag gggttccgct cccactggtc agttatggag gatcggcgct aattgtgctg
    14161 atggctgggt tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc
    14221 gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct
    14281 cgcggcatgt acaagcgatg atggtcagca acagacagta agtgtaccgc agcctgcggt
    14341 atgtaacggc cccatagttg aaattagcgg ggcggacccg cgtttcgaac cactgaacgc
    14401 gacggcaaat caggattacc agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc
    14461 tcgatttatc caggcgggac tggcggcaat ctatgatgcc gaaccaggca gtaacctgac
    14521 ggcctctggc gaagctttcg atccgacaca gctgacggcg gcccatccaa cgcttccgat
    14581 ccccagctac gccagaatca ctaacctggc taacgggcga atgatcgtgg tgcgcattaa
    14641 tgatcgcggt ccttacggca acgaccgcgt tatttcgctt tctcgcgcag cagctgaccg
    14701 tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg attattgtcg cccaggatgg
    14761 ttcgctttct ggtcctggta tggcttgtac cacagtcgcc aaacagactt acgccctgcc
    14821 tgcacctccc gatttaagcg gtggcgcggg aacaagttca gtgtctggcc cgcagggtga
    14881 cattcttccg gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac
    14941 cagcagcggt ttcctcggcg caccaacgac cttagcgcca ggtgtactgg aaggcagcga
    15001 accgacgcct gctccacagc ccgttgttac agctccgtcg acaacgcctg caacctcgcc
    15061 tgcaatggtg acaccgcaag ccgcctcgca aagcgccagc ggcaacttta tggtgcaggt
    15121 cggggccgta agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt
    15181 cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt
    15241 cgccaacaaa gccgaagcca gtaccttgca gcaacgtttg caaaccgaag cccaattaca
    15301 gtcatttatt accaccgcgc agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa
    15361 atgacgttaa caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt
    15421 agggcacttt ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt
    15481 ccgctcgtat catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg
    15541 ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg
    15601 cggagtccta catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag
    15661 atgtccgccg cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg
    15721 caatgaaagc cggtaaattt aaagaaactg atttagtcac tattggcaac gacgcatggg
    15781 ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg
    15841 ttccggtttc tcagctgatc cgcggtataa acctgcaatc gggtaacgat gcttgtgtcg
    15901 ccatggccga ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg
    15961 ttaacgccct gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg
    16021 gtcagtacag ctccgcgcgc gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac
    16081 cgaatgaata ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga
    16141 accgtaacgg cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca
    16201 ctgacaaagc aggttacaac cttgttgctt ctgcgactga aggccagatg cgcttgattt
    16261 ctgcggtgat gggcggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa
    16321 cctggggctt ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct
    16381 ctgaaccggt ttggtttggt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt
    16441 acctgaccat tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca
    16501 gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg
    16561 atggcaaaac gatcgaacaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact
    16621 tcttcggcaa aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat
    16681 taaacacttg aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaactcccgc
    16741 cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt
    16801 aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct
    16861 gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca
    16921 acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact
    16981 catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg
    17041 gttctgtaac tcgcttctcc gttacccggc ccctggtcgg gtaactcccc tctcctcgct
    17101 gtgatatact tttctcccac ttttactcat tctccacgga gatgccgttt tgtatcagga
    17161 taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc aggctatgca
    17221 tgaattcacc gatacccgcg atgatagtac ccttgatgaa atctggctgg tcgagcacta
    17281 tccggtattc acccaagggc aggcaggaaa ggcggagcac attttaatgc ccggtgatat
    17341 tccggtgatt cagagcgatc gcggtgggca ggtgacttat cacgggccgg ggcaacaggt
    17401 gatgtatgta ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt
    17461 gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc
    17521 tgacgcgcca ggtgtctatg ttggggaaaa gaaaatttgc tcactgggtt tacgtattcg
    17581 acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt
    17641 acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc
    17701 cgaagcgacg actaataata ttgcaccacg tttactggaa aatattttag cgctactaaa
    17761 caatcctgac ttcgaatata ttaccgctta atttcatacc tcaatggccc aatttacact
    17821 gggtcattac tcataacacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct
    17881 ttatcacttg cagaaatgcg atgctacgcc ttgctccgta actcacccca ttgattaact
    17941 tattgaaata gttgattatg atgagcaaaa gcgttccgtt ccaattagac ataagattat
    18001 tgtaattttt ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta
    18061 ataatcaaat tgaatcctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta
    18121 caaccttacg aaatattgat cttaatcttc tgactatttt tgaagctgta tatgtacata
    18181 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
    18241 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
    18301 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
    18361 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
    18421 ccccctcggt cggagctctg gtccttcctg tcatttatcg ggcgattaaa actcactatc
    18481 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
    18541 aaaccgatct catcatagat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
    18601 tgttcaccga caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag
    18661 atgacagaga gactatcgac aacgctgcac atgtactcct gttaccggaa ggacaaaatt
    18721 tcagcggtct gcggcagaga attcaagaga tgtttccgga ccggcagatc agcttcacca
    18781 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
    18841 ccagccgttt ttataacctg tttagccgtt gctggccgct ggaaaaactg ccttttccgt
    18901 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc
    18961 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
    19021 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
    19081 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
    19141 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
    19201 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
    19261 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
    19321 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
    19381 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
    19441 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tgggcgctat ttgtacccgc
    19501 cgttgtccgt tctgtgacgt tgcccacggt cgcccggtag ctcctgatgc caatgagcca
    19561 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
    19621 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
    19681 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgtatg
    19741 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
    19801 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
    19861 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
    19921 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
    19981 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
    20041 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
    20101 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
    20161 gggatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
    20221 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
    20281 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
    20341 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
    20401 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
    20461 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
    20521 cttaaaaacc aaagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
    20581 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
    20641 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
    20701 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
    20761 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
    20821 atccagcgca cgagcggcaa gcaacgttga ccagtggtgc tctttgagcg gcccgcgaac
    20881 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
    20941 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
    21001 cggagcaatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
    21061 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
    21121 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
    21181 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
    21241 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
    21301 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
    21361 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
    21421 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
    21481 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
    21541 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
    21601 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
    21661 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
    21721 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
    21781 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
    21841 cattctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
    21901 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
    21961 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
    22021 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
    22081 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
    22141 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg
    22201 tggtacgctt tgtccagtgc aactttacta aaaag
//
LOCUS       DAZGXS010000074        21700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGXS010000074 DAZGXS010000000
VERSION     DAZGXS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            410..690
                     /gene="drpB"
                     /locus_tag="ACHKG5_002913"
                     /pseudo
     CDS             410..690
                     /gene="drpB"
                     /locus_tag="ACHKG5_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
     gene            730..1425
                     /gene="yedJ"
                     /locus_tag="ACHKG5_002914"
     CDS             730..1425
                     /gene="yedJ"
                     /locus_tag="ACHKG5_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0868981.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1492..2910
                     /gene="dcm"
                     /locus_tag="ACHKG5_002915"
     CDS             1492..2910
                     /gene="dcm"
                     /locus_tag="ACHKG5_002915"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0868982.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2891..3361
                     /gene="vsr"
                     /locus_tag="ACHKG5_002916"
     CDS             2891..3361
                     /gene="vsr"
                     /locus_tag="ACHKG5_002916"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0868983.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3350..4270)
                     /gene="yedA"
                     /locus_tag="ACHKG5_002917"
     CDS             complement(3350..4270)
                     /gene="yedA"
                     /locus_tag="ACHKG5_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0868984.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4443..5360
                     /gene="yedI"
                     /locus_tag="ACHKG5_002918"
     CDS             4443..5360
                     /gene="yedI"
                     /locus_tag="ACHKG5_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0868985.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5439..5621
                     /gene="yodC"
                     /locus_tag="ACHKG5_002919"
     CDS             5439..5621
                     /gene="yodC"
                     /locus_tag="ACHKG5_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0868986.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5792..7486
                     /gene="dgcQ"
                     /locus_tag="ACHKG5_002920"
     CDS             5792..7486
                     /gene="dgcQ"
                     /locus_tag="ACHKG5_002920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0868987.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7483..8298)
                     /gene="yedP"
                     /locus_tag="ACHKG5_002921"
     CDS             complement(7483..8298)
                     /gene="yedP"
                     /locus_tag="ACHKG5_002921"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0868988.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8596..8823)
                     /gene="yodD"
                     /locus_tag="ACHKG5_002922"
     CDS             complement(8596..8823)
                     /gene="yodD"
                     /locus_tag="ACHKG5_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0868989.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8986..9174
                     /gene="dsrB"
                     /locus_tag="ACHKG5_002923"
     CDS             8986..9174
                     /gene="dsrB"
                     /locus_tag="ACHKG5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0868990.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9218..9841)
                     /gene="rcsA"
                     /locus_tag="ACHKG5_002924"
     CDS             complement(9218..9841)
                     /gene="rcsA"
                     /locus_tag="ACHKG5_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0868991.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10131..10916)
                     /gene="fliR"
                     /locus_tag="ACHKG5_002925"
     CDS             complement(10131..10916)
                     /gene="fliR"
                     /locus_tag="ACHKG5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0868992.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10924..11193)
                     /gene="fliQ"
                     /locus_tag="ACHKG5_002926"
     CDS             complement(10924..11193)
                     /gene="fliQ"
                     /locus_tag="ACHKG5_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0868993.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11203..11940)
                     /gene="fliP"
                     /locus_tag="ACHKG5_002927"
     CDS             complement(11203..11940)
                     /gene="fliP"
                     /locus_tag="ACHKG5_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0868994.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11940..12305)
                     /gene="fliO"
                     /locus_tag="ACHKG5_002928"
     CDS             complement(11940..12305)
                     /gene="fliO"
                     /locus_tag="ACHKG5_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0868995.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12308..12721)
                     /gene="fliN"
                     /locus_tag="ACHKG5_002929"
     CDS             complement(12308..12721)
                     /gene="fliN"
                     /locus_tag="ACHKG5_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0868996.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12718..13722)
                     /gene="fliM"
                     /locus_tag="ACHKG5_002930"
     CDS             complement(12718..13722)
                     /gene="fliM"
                     /locus_tag="ACHKG5_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0868997.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13727..14191)
                     /gene="fliL"
                     /locus_tag="ACHKG5_002931"
     CDS             complement(13727..14191)
                     /gene="fliL"
                     /locus_tag="ACHKG5_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0868998.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14296..15423)
                     /gene="fliK"
                     /locus_tag="ACHKG5_002932"
     CDS             complement(14296..15423)
                     /gene="fliK"
                     /locus_tag="ACHKG5_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0868999.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15420..15863)
                     /gene="fliJ"
                     /locus_tag="ACHKG5_002933"
     CDS             complement(15420..15863)
                     /gene="fliJ"
                     /locus_tag="ACHKG5_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0869000.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15882..17255)
                     /gene="fliI"
                     /locus_tag="ACHKG5_002934"
     CDS             complement(15882..17255)
                     /gene="fliI"
                     /locus_tag="ACHKG5_002934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0869001.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17317..18003)
                     /gene="fliH"
                     /locus_tag="ACHKG5_002935"
     CDS             complement(17317..18003)
                     /gene="fliH"
                     /locus_tag="ACHKG5_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0869002.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(17996..18991)
                     /gene="fliG"
                     /locus_tag="ACHKG5_002936"
     CDS             complement(17996..18991)
                     /gene="fliG"
                     /locus_tag="ACHKG5_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0869003.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18984..20642)
                     /gene="fliF"
                     /locus_tag="ACHKG5_002937"
     CDS             complement(18984..20642)
                     /gene="fliF"
                     /locus_tag="ACHKG5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0869004.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20857..21171
                     /gene="fliE"
                     /locus_tag="ACHKG5_002938"
     CDS             20857..21171
                     /gene="fliE"
                     /locus_tag="ACHKG5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0869005.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(21196..21336)
                     /locus_tag="ACHKG5_002939"
                     /pseudo
     CDS             complement(21196..21336)
                     /locus_tag="ACHKG5_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            21520..>21700
                     /locus_tag="ACHKG5_002940"
     CDS             21520..>21700
                     /locus_tag="ACHKG5_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFP0869006.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSE"
BASE COUNT         5176 a         5544 c         5730 g         5250 t
ORIGIN      
        1 aaagatgggg ggtatcaatg attgttgcgc taaacggctg gtgtgatcac gcgcacatta
       61 atgaaggcat aaaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa
      121 aaggctaata acggaagcat catgacacag aataataaaa aggtataatt gtaatcagtg
      181 ctaatatatt gattgaatga attaataaag ccgatctcaa agataaataa aatcgctcaa
      241 aacgattttt cctgtattca ctgccgttgc gcaaaaatta tctatttgtt caaaaaatga
      301 gtgagtcttg actggctcat ccaatgtgga aaaatgtgac ttttatcacg taatagtact
      361 aagtctgaat tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag
      421 actctctcgc agccctggcg gcaaactggc tttgtgggct ttttatacat ggtgtggcta
      481 tttcgtctgg gcaatggcgc gttacatctg ggtgatgagc cggatccccg atgcccccgt
      541 gtcgggattt gaaagcgatt taggctctac cgcgggaaag tggttagggc actggtcgga
      601 tttttattta tggctctggt cggggcattg ttaggcagca ttgcctggta cacacgacct
      661 cgtcccgcac gtagcagacg ctatgaataa aaactgctaa ggtggcgact ctttggcaac
      721 ggagcgcgta tggacttaca acactggcag gcacagttcg aaaactggtt gaaaaaccat
      781 catcagcacc aggacgcggc ccatgacgtg tgtcattttc gccgcgtctg ggctacggca
      841 cagaagctgg cggcagatga tgacgtcgat atgctggtga ttttaaccgc ctgttatttc
      901 cacgatattg tgagcctggc gaaaaatcat ccgcaacggc agagttcttc aatcctggcg
      961 gcagaagaga cgcgtcgcct gctccgtgaa gagttcgtgc aatttccggc ggagaaaatc
     1021 gaggccgttt gtcatgccat tgccgctcac agtttcagcg cgcaaatcgc ccccttaaca
     1081 acggaagcta aaatcgtcca ggatgcggat cggctggaag ccctgggggc gattggcctg
     1141 gcgcgcgtgt ttgccgtttc aggggcattg ggcgtggcgc tgtttgatgc cgaagatccg
     1201 ctcgcacagc atcgcccgct tgatgataaa cgctacgcgc tggatcattt ccagactaag
     1261 ttgttgaagc taccgcaaat catgcaaacc gtaagaggca agcagctggc gcagcacaac
     1321 gcgcattttt tagtcgagtt tatggcgaag ctcagtgccg aactggcggg ggagaatgaa
     1381 ggtgtcgatc acaaggtgat agatgcgttt tcacccgcgg gctgagcgcg tggccctaaa
     1441 tggctgtaat tatgttaacc tgtcgaccat ctcagatggc cggttaaatc tatgcaggaa
     1501 aatatatcag taaccgattc atacagcacc gggaatgccg cacaggcaat gctggagaaa
     1561 ctgctgcaaa tttatgatgt taaaacgctg gtggctcagc ttaatggtgt gggtgagaat
     1621 cactggagcg cggcaatttt aaaacgtgcg ctggcgaatg actcggcatg gcaccgttta
     1681 agtgagaaag agttcgccca tctgcaaacg ttgttaccca aaccaccggc acatcatccg
     1741 cattatgcgt ttcgctttat cgatctattt gccggaattg gcggcatccg tcgcggtttt
     1801 gaatcgattg gcggacagtg cgtgtttacc agcgaatgga acaaacatgc ggtacgcact
     1861 tataaagcca atcattattg cgacccggcg acgcatcatt ttaatgaaga tatccgcgac
     1921 atcaccctca gccataaaga aggcgtgagt gatgaggctg cggcggaaca tattcgtcaa
     1981 cacattcctg aacacgatgt tttactggcc ggtttccctt gtcagccatt ttcgctggct
     2041 ggcgtatcga aaaagaactc gctcgggcgg gcgcacggtt ttgcctgcga tactcagggc
     2101 acgctgttct ttgatgtggt gcgcattatc gacgcgcgtc gtccggcgat gtttgtgctc
     2161 gaaaacgtca aaaacctgaa aagccacgac cagggtaaaa cgttccgcat catcatgcag
     2221 acgctggacg aactgggcta tgacgtggct gatgcagaag ataacgggcc ggacgatccg
     2281 aaaatcatcg atggcaaaca ttttctgccg cagcaccgtg aacgcatcgt gctggtgggt
     2341 tttcgtcgcg atcttaatct gaaagccgat tttactctgc gtgatatcag cgaatgtttc
     2401 cctgcacagc gagtgacgct ggcgcagctg ttggacccga tggtcgaggc gaaatatatc
     2461 ctgacgccgg tgctgtggaa gtacctctat cgatatgcga aaaaacatca ggcgcgcgga
     2521 aacggcttcg gttatggaat ggtttatccg aacaatccgc aaagcgtcac ccgtacgctg
     2581 tctgcgcgtt attacaaaga tggcgcggaa attttaatcg atcgcggctg ggatatggcc
     2641 acgggtgaga aagactttga cgatccgctg aatcagcaac atcgtccacg tcggttaacg
     2701 cctcgggaat gcgcgcgctt aatgggtttt gaagcgccgg gagaagcgaa attccgcatt
     2761 ccggtttcgg acactcaggc ctatcgccag ttcggtaact cggtggtcgt gccggtcttt
     2821 gccgcggtgg caaaactgct tgagccaaaa atcaaacagg cggtggcgtt gcgtcagcaa
     2881 gaggcacaac atggccgacg ttcacgataa ggccactcgc agcaaaaata tgcgcgcgat
     2941 tgccacgcgt gatacggcga ttgagaagcg cctcgccagt ctgttaaccg ggcagggcct
     3001 ggcgtttcgc gttcaggacg ccagtctgcc cggacgcccg gattttgtcg ttgatgaata
     3061 tcgctgcgtg atatttaccc atggctgctt ctggcatcat catcactgct atctgtttaa
     3121 agtgcctgcg actcgaaccg agttctggct ggagaagata ggtaaaaatg ttgagcgcga
     3181 tcgccgcgat atcagtcgct tgcaggaact cggctggcgc gtattgattg tctgggagtg
     3241 cgcgttacgt gggcgcgaga agctgaagga tgaagcgctt accgagcgtc tggaagagtg
     3301 gatctgcggc gaaggagcca gcgcgcagat cgacacgcag gggatacatt tactcgcttg
     3361 atgcgtcctg aataactgga gcaactacgg gttttgccgg aaagagatat tttcccaacg
     3421 tgaccagtac caccgcgaag acaattacgc cgagcgccag ccattcaatc ttcgacagtg
     3481 tttctccacc cagtcccgta cccagcaaga ccgcgaccac cgggttaacg taagcgtagc
     3541 tggtggcgag agccggactg acattacgga ttaaatacat ataagcgttg atggcgataa
     3601 tcgaaccaaa cagcgccaga tagccgaccg caaggaagcc tgaaagggaa gggagcgccg
     3661 tcagtttttc acccgcaatc atcgacgcga tcattaacac cacgcctgcc gccagcatct
     3721 caatcgcacc cgccatcatc cctacaggta aggtaatgcg cgagccataa actgagccaa
     3781 acgcccagct aatcgagccg attaaaatca gaatcgcgcc ccacggattg ccgcttaaat
     3841 ttccaccgct attgagcatg atgattccgg caagcccaat ggcaataccc acccattcca
     3901 gtttgcgcgt tttaatgcca aacaggcggc tgaagcacag ggtaaagagg ggcacggttg
     3961 caaccactac ggcggcgatg ccggaaggaa cattttgatg ttcggcaacc gtcaccatgc
     4021 cattaccgac agccagcaat aacaggccaa tcagcgcggc attgagcagc ggacgtagcg
     4081 gggggagttt gtgtccgcgc agtagtaaaa atgccagcaa taaaataccg gctgccagga
     4141 atcgaacacc cgccatcatt aacggaggcc agctttccac gccaatccga atgacaaaat
     4201 aggttgagcc ccaaatgata tacaacgcaa acagcgcgcc aaaaagcggt aacaactggc
     4261 ggaaacgcat aatccctcac ggtggaaata aaaaggtggt tcatagtaaa cgtgaaaatc
     4321 attctgctgg cgagagatat aattgcactt gattgttaaa aaaatgttga cctgtgaagc
     4381 aagtatcaga gctgccgttt ttgcttcata cttacaccct tcaacaataa aaatgagagg
     4441 gaatgctttt ggccgggagt agtttactga cgttgctcga tgatatcgcc acactgctgg
     4501 acgatatctc cgtgatgggc aaactggcgg cgaagaaaac cgccggtgta ttaggggatg
     4561 acttatcgct caatgcgcaa caagtttcag gcgtgcgggc caaccgggaa cttcccgtgg
     4621 tctggggcgt ggcgaaagga tcgctgatta ataaagtgat tctggtgcca ctggcgctga
     4681 tcatcagtgc gtttatcccg tgggcgatta cgccattgtt gatgatcggt ggcgcgtttc
     4741 tctgctttga aggggtagag aaagtgctgc atatgctgga ggcgcgtaaa cataaagaag
     4801 atccggcgca gagccagcag cgtctggaga agctggcggc gcaggatccg ctgaagtttg
     4861 aaaaggacaa aataaaaggg gcgattcgta ccgattttat attgtctgcg gaaatcgtcg
     4921 ccatcacgct ggggattgtg gccgaagcgc cgttgcttaa tcaggtgctg gtgctttcag
     4981 gcatcgcgct ggtagtgacc gtgggcgtct acggtctggt aggggttatc gttaagattg
     5041 atgacctggg gtattggctg gcggaaaaat ccagcgcgct gatgcaggca ttaggtaaag
     5101 gattattgat tatcgcgccc tggctgatga aagcgttatc gattgtcggc acgctggcga
     5161 tgttcctcgt cggcggcggg attgtggtac atggtattgc gccgctgcat cacgccattg
     5221 aacatttcgc cgggcagcaa agtgcagtgg tggcgatgat attaccgact gttttaaatc
     5281 tgattcttgg atttatcatc ggcggcatcg tggtgctggg agtgaaagcc gtagcgaaaa
     5341 tgcgcggtca ggtacattaa tactcgcaca ggctattatg caaaattcgc catcttcaac
     5401 taaggtgaac aagtttcact cgaaaaaagg aggcgagtat gagctttatg gttagtgagg
     5461 aagttacggt taaagagggc ggcccgcgga tgattgtcac tggatactcc agcggtatgg
     5521 ttgagtgtcg ttggtatgac ggttacgggg tcaagcggga agcttttcat gaaaccgagc
     5581 ttgttccagg ggaggggagt cgttctgcgg aagaagtttg aaagggtatc attaagacca
     5641 ggccggatca catccggcct ggtgtgccaa tgcctgcaat gaatgatagt cgcgtcttat
     5701 catgccacca ttataaaatt atcgctgttt tgcggtacgc tatcgccagg ttatgccaga
     5761 atcataaaaa agcaggtggg gagtcgtcag ggtgcagcac gagacaaaaa tggaaaacca
     5821 gagctggttg aaaaaactcg cacgccgcct agggcctggt catatcgtta atctctgctt
     5881 tatcgtggta ttgctttttt ccaccttgct gacctggcgt gaagtagtgg tgctggaaga
     5941 tgcctatatc tccagccagc gtaatcatct ggaaaacgta gccaacgcgc tcgataagca
     6001 tttgcagtat aacgtcgaca aactgatctt tttgcgtaat ggcatgcgcg aagctctcgt
     6061 agcgccactg gatttcacct ctctgcgtga tgctgtaacc gagttcgaac agcatcgcga
     6121 cgagcacgcc tggcaaatcg aactcaaccg acgacgcacc ctgtcagtca atggcgtatc
     6181 ggatgcatta gtcagcgagg ggaatctcct gtctcgcgaa aatgaaagcc tcgacaatga
     6241 aattaccgct gcactggaag ttggttactt gctgcgactg gcacacaact cctcgtcgat
     6301 ggtagaacag gcgatgtatg tctcgcgtgc cggattttac gtttcgacgc agccgacctt
     6361 gtttacgcgc aatgtcccga cgcgttatta cggctatgtc acccaaccct ggtttatcgg
     6421 ccattcacaa cgagaaaatc gtcaccgcgc ggtacgctgg ttcacttcgc aaccggaaca
     6481 cgccagcaat actgaaccgc aggttaccgt cagtgttccg gtagacagta ataactactg
     6541 gtatggcgtg ctggggatga gtattcccgt gcgtactatg cagcaatttt taagaaacgc
     6601 catcgataaa aacctcgatg gtgagtatca gctctatgac agtaagctga gatttttgac
     6661 ctcttccaat cctgaccatc caacagggaa tatttttgat cctcgtgaac tggccttgct
     6721 ggcgcaggca atggaacatg acacgcgggg cggcattcgt atggacagtc gctatgttag
     6781 ctgggaacgt ctggaccatt tcgacggtgt gctggtgcgc gtccatacgt taagcgaagg
     6841 cgtgcgcggc gatttcggca gtatcagcat tgcattaacc ctgctgtggg cgctctttac
     6901 caccatgtta ctcatctcct ggtatgtgat tcgccggatg gtcagcaaca tgtatgttct
     6961 gcaaagctcg ttgcagtggc aggcgtggca cgacacctta acgcgtttat ataaccgtgg
     7021 cgcactgttc gaaaaagccc gtccgctcgc gaaattgtgt cagacgcacc aacatccttt
     7081 ttctgtcatt caggtcgatc ttgaccattt taaagcgatt aatgaccgct ttggtcatca
     7141 ggcgggcgac cgtgttcttt ctcatgctgc cggattaatt agcagttcct tgcgtgcgca
     7201 ggacgttgcc gggcgggtcg gtggtgagga gttttgtgtg attctgccag gcgcgagtct
     7261 gacggaggct gcggaagtcg cagaacgtat tcgcctgaag ttaaatgaaa aagagatgtt
     7321 gattgctaag agtacgacga tacgcatcag tgcctcgttg ggggtaagta gcagcgagga
     7381 aaccggtgat tatgattttg aacaactcca gtcactggcc gaccgtcggc tttatctcgc
     7441 taaacaggct gggcgcaatc gggtattcgc gagcgataac gcttaacggg cggagaaaaa
     7501 atggtccagc ccttcacgcc atccttccgg tccttcacgc tgcgttcgcc agacgcgggc
     7561 cggatcctca tcatgcagat gcaccccttc acggtttagc cctttcacaa tcaccgcgta
     7621 atccattacc tccagtaagg gcgcatcgtt tggcccatcg cccaggccaa gtgtggttgg
     7681 gcgtttgcct gacaattgtt gataggtcgc gataatccag ttggcagcct gatcttttcc
     7741 ggcagaggca tccaggacgt gccagaagcg cgcaccttgc ataaactgta agcccagttc
     7801 gttcagacga gcggtaaatt gtgccatacg ctcgtcactg tcgcgccaga ttagcgttac
     7861 cgacgcctca tgaagctgcg tcagcgccgc ctggctacgg cttaatcccg tccattcggc
     7921 gatggttgca tcgtcgacat catcaaaagt cgtgaattta aaatgttctt tctcgcgtag
     7981 cgtatttaaa acctggctga tttcgccatg gctaatacct gagatgatgc gtggaaaacc
     8041 gtctatctcc tgccattgtt cagcaagctg gatcactgcg ccattctctg caatcagcgg
     8101 taaaccttgt agccccaaca ttttttgcaa gtacagcatt tccgctgatg ttttactgct
     8161 acagagaatg acgggaacat ttgcttcgcg taaacggctg agccaggggg ctgccggttg
     8221 ccagtcataa ctatgactgt ccagcagggt gccatcaaga tcgctaaaaa ccagtagtgg
     8281 ttgttgaatt gaaaacatag tcgcgcagta ctccccttac caggatgatt ctcaaaacag
     8341 cacaaattat caaagatgat tttttcggga gcgatcccgc tgacgccaga gccaccaagg
     8401 ggtgacgtgc gtcacatttc tattcataag tagcgttaat cattcatata gcgaatattt
     8461 tcttgtcagc gaaaaaaatt gcggataagg tgatgaacac atcagatttc ctggtgtaac
     8521 gaatttttta agtgcttctt gcttaagcaa gtttcatccc gaccccctca gggtcgggat
     8581 ttttttattg tgcattcaac gattcacttc agagacgctg aagtcatgaa tatccagttc
     8641 gaaggcatcc gccaattcac gccatgtatg atattcacgt ccatcgacgc taacgtgttc
     8701 agaatcgttc tggctgcgat gaacttcgct ttcgctaatt tcattgatcg ccgccagcag
     8761 ggcatcgaca tcgacgctga cctcacgttt tgcggtatcg ctgtactctt ttgcggtttt
     8821 catcatcttt cctcgcaacc gttactctgt tgttgccgcc agtcgttaag tccgcctggc
     8881 atctcttaag tatagaccgt cgagaaaatc agcactcgcg cgcgcctggc gctgccagaa
     8941 aaacgaaatt gttctacact ggcacaaagc cacaggagga aaacgatgaa ggtgaatgat
     9001 cgggtaacag tcaaaacgga tggcggtccg cgtcgtcctg gcgtggtact ggcagttgag
     9061 gagtttagtg aaggcacaat gtacctggtt tcgctggaag actacccgct cggcatctgg
     9121 ttctttaatg aagcagggca tcaggacggt atctttgtgg agaaagcaga gtaatctaat
     9181 tcagcctgac tggtgggaaa ccaccagtca gaatgtgtta gcgcatgttg acaaaaatac
     9241 cattagtcac attatccgtc agtcggacga catggtagat aacctgttta ttatgcgttt
     9301 tgatcttacg tttaatatta cctttatgcg atgaaacggt cttggctttg atattcattt
     9361 ggtcggagat ttgaatggtt ccctgacctg ccatccacat tcgcaacata ctcgattcgg
     9421 ttcggctcaa tgataacgtc ggcatattta aaaacgaggt tatcgttgtc tcttttttca
     9481 gaatatcgcc aaggatatcg tcgagagatt ccggtttaat cgatttagaa ctgatcaata
     9541 aattttttct gaccaataga tattcatcaa aatgaacatt ggcaattgcc ataaaaacga
     9601 taaataacgt attgggatgt tgattaatga tgagcttgat acgctgactg ttagaagcat
     9661 cgtggatgaa acagtcctca ttaataaaca ccactgaagg gcgctgtgaa tcacaagcta
     9721 tggcaaggtc atcaacggtt tcaatgtcgt tgatttctct ttttttaacc cctctactca
     9781 acagataccc ggttaaacct agtcgggtgt aactacataa atccataata atcgttgaca
     9841 tggcataccc tcactcaatg cgtaacgata attcccctta cctgaatatt tcatcatgac
     9901 taaacggaac aacatgggtc acctaatgcg ccactctcgc gatttttcag gcggacttac
     9961 tatcccgtaa agtgttgtat aatttgcctg gaattgtctt aaagtaaagt aaatgttgcg
    10021 atatgtgagt gagcttaaaa caaatatttc gctgcaggag tatcctggaa gatgttcgta
    10081 ggtattattt ttcggataat ccttaggata acatgataaa cgttacggaa ttatattaat
    10141 ggcaattcac taataatatc agccagcaaa ttaaaaattt cactgaataa atgttcgcaa
    10201 aaaggcgcaa ttaacggcat taatgccgcc attaaagaga tgccgacagt cagagttaat
    10261 ggaaatccaa taacaaaaat ggataattgc ggggccatac gattaagtaa acctaatgcc
    10321 agattcagtg tcagcagcag agtaatgagc ggtaacgcca gcatcagccc gttaaggaaa
    10381 atcaaactcc ctgctttggt gagtgccaga aacgcattgc tgttcaacgg ttcgccacca
    10441 atcggcaggg tgtgaaaggt atcgaccagc agtgaaatca accataaatg accgttaaat
    10501 gtcaggaaca gcagtaacgc cagcatatcc atgatacgcg ctaaaacggg catattaaga
    10561 tggctggccg gatcgacaaa cgtcgcaaat gacagcccca tttgcagacc gataatttcg
    10621 ccagcggttc gcacagcggc aaaggcaaat tgcatggtaa aaccaagcgc aatgccgatc
    10681 aggatctgct gcacggccag ccacagagca aagaacgaaa aaacaggaac atcgttggca
    10741 ggtaatgatg gggcaatggc gaacgtgatc atcattgcca gacccagttt tacccgtttc
    10801 ggtacgctgc gttcgctcag aatcggcgcg gtggagatca gcgccagcac gcgcagtaac
    10861 ggccagaagt acaggcttaa ccaggatagc cattgttcgc ttgtcacctg caacatagta
    10921 cggctacccg atgatatacg gcaggttagt gaacaaggtg cggacgtaat ccagcaacag
    10981 attgagcatc cacggtccgg caataataat ggcgataaat acggcgatga ttttcggaat
    11041 aaacgacagc gtcatttcgt taatctgcgt ggcggcctgc aaaatactga tgataaggcc
    11101 cgtgaccaac gctaccaaca atagcggggc agccagtgcc agcgcgactt tcatcgcttc
    11161 agtccccatc atcatgaccg attcaggtgt cattttgcct ctctagctgt aaaagctctg
    11221 cgccagcgaa ccgaccagca attgccagcc atccaccagt acaaacagca tcagtttaaa
    11281 gggcagagca atggtggcgg ggggaaccat catcatcccc aatgccatca acacgctggc
    11341 tatcaccagg tcgataatca aaaaagggat gaaaatcgtg aagcctatct gaaatgcggt
    11401 tttcaactcg ctggtcacgt aggccgggag caaaatgcgc atcggcacgg cttcaggtcc
    11461 ctgcaacggg ccggtattcg ccagtctggc aaacaaccct aaatctgcct cacgggtctg
    11521 acgcagcata aactcacgca gcggctgcgc ccctttttcc agcgcctcct gcattgatat
    11581 tttctcttcg ctgaatggct ggtacgcatc gacataaatt ttgtcgatca ccggtgacat
    11641 aataaaaaag gtcaaaaaca gtgccagccc cagcaatacc tggttaggtg gcgcggaggg
    11701 tgttcccagc gcgttacgca ataaaccaaa aacaatgatg atgcgggtga aactggtcat
    11761 catcagtaaa attgccggaa taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg
    11821 gagcgaccag ctttgtccac cgccaggcag cggctggctg gtgatacccg gcagttgcgc
    11881 gaaggcgagg ggcgtaatca gccagagaag gacaggtgcg acagacaata aacgacgcat
    11941 caggatctcc cgctacgctt aagcaaattt ttcatgaccg actgaaaatc ggtctgcggt
    12001 atctcttctg ttggtgctga agggggaagt ttatgcagca gattgatttg ccctgcggta
    12061 acgccgagca ccagccgtgc atcttccaca tcgaccacca caacccgttc acgcgcgccc
    12121 agcgaagcac tggcgctaat tttcagaccg ttaacgccag tgcgtttcgg ggcaaatccc
    12181 aaccgtttta ccagccaggc agcagcgagg atcagggcaa taatggcgat gagtgcgccg
    12241 ctcacctgca gcagtggcgc agcagaaacc ggcgcggaag attgcacagt agcgtggtta
    12301 ttcatcacta acggctcagg cggcgcattc gctcagacgg agtaatgata tcggtgatcc
    12361 gcacgccata tttatcggca acgaccacca cttcgccctg ggcgattaaa taaccgttga
    12421 tcagaatatc cagtggttcg cccgccagac cgtccagcgc cacgacggac ccttgcgtca
    12481 gacgcaacag ctctttgatg gtcattcgtg tgcggcccag ctcgacggtc agcttgaccg
    12541 gaatatccat aatcaggtcg atatcctgca acgttccgct gacatcacca ccgccaaatt
    12601 gctggaacac cgcgtcggca gcgcttttgc tgctggttga tttttgttcg ctcaacgctt
    12661 cagcccacag atcgtccatt gcgccgttgt tgtcatcggc cggattattc atgtcactca
    12721 tttgggctgt tcctcgttca gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa
    12781 cgcatactga ccgttgagag tgccgtactg actggtcagc accgggacgc cgtcaacatg
    12841 ggcgatgatg cggtcgggtt tttctatcgg caggacgtca ccgggtttca gttttaaaat
    12901 ctgcgacagg cgtagcgaga tatcggcaaa gttggcgacc agctccagct gtgaatgctg
    12961 cacctggcgc accaggttat cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg
    13021 cgggttaacc aacaattccc gtagcggctc gatcatgctg aatggcaggc agatattaaa
    13081 ttcgccggtc aggttgccaa tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg
    13141 cgaggtggtg atattggtaa atttcacctg catttccgaa cgcacgtact caacttccag
    13201 cggattaatc gccttccatg cgtcgctata gccttcaagc gccagtttca acatgcggtt
    13261 gatgacgcgc tgttcggtat gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc
    13321 atcgccgcca aacaggttat ccacggcgat aaacaccaga ctcggtgaga acaccaccag
    13381 cccagtgccg cgcagcggtt ttagatggat aaggttcagg ttggtcggca ccggcaggtt
    13441 gcgggcaaat tcatggtacg gctgaatgcg gatggccccg acggttatat ccgggctacg
    13501 acgcagcagg ttgaacagcc ccatacgaaa atggcgggca aagcgctcat taatgatttc
    13561 cagcgcctgc aaacgttcgc gcacaacccg tcgttgggta ttcggatcgt acggacgaat
    13621 gtcactttcg ccgctaacac tggctgtcgg ttcgtctttg acttcgctgt caccattcaa
    13681 cagcgcatca atttcagctt gagaaagaat actatcgccc atgtcgttat cgcagaataa
    13741 aagcggtata cagcacgtcg gtgacatcct gtttcggttg cccggcaaca agcggggtgg
    13801 aaagtgtggt tttaatctcg gcaatcaggt ttttcttgcc ttcttctgtc gccagtacgg
    13861 cagcatcctg acgcgaaaac agtaacagca aacggctacg gacttccggc aaatactcac
    13921 tcagccgcga gcgggtagct tcatctttca ggcgcagggt tatgccgata taaagcacgc
    13981 gatccgcatc gcccagattg accgtaaagg tatccagcgc atagaagacc ggggagggca
    14041 cgacgcgttg ctgcgctttg tcgtcagcag caacctgatg cgaatgccag tagctgtaac
    14101 ctgcgctggc acaggccgcg agggtaatga ataccagaat cgggatccaa agcgatcgct
    14161 tgcttttctt gcttatcgcg taatcagtca tgtgttgcgg tcttcctgtg tcgctactgc
    14221 ttatcggcta attatcctgc gtcttgagcg caacaaagcg gggaaaagac gcggattacg
    14281 gtgctacctc tgacgttagg cgaaaatatc aacgccgctg ttgcctgtta cgcgcccttg
    14341 taaagagacg ggaaccggaa gcgtatcgtc gtcttccccc gccagaggtt catggtttac
    14401 tgtgcgttgg ctttgctgtt gttgggaagc ggcctgctgc tgaccactaa agctttcgcc
    14461 actgatgttg ctttgcccta actgaatgcc actttcggcc agctgcgtac gcagtaccgg
    14521 cagcgctgct tccagggcgg cgcgtacatg ctgatgcgat gaaaccatct ggatttgcgc
    14581 ctggttatca tccactttga gggagatttg cacttcacct aaatcctgcg ggtgcagacg
    14641 caactctgca ctttgttgcc cctggcgggt gaacagcgaa atatgctggc ttaatgattg
    14701 ttgccattcg tgagaaccca gcggtgcact caaaacaggc gcggcgacgg tgggcagtgg
    14761 ctgtgtctgg tgtggagtga ttagcgggct ggcggcagca gtcaccggcg aaggcgtgct
    14821 gatgacttcc gctttactct gggcttctgc tacctgcggt gttaatggct gagctggtgt
    14881 gccgggggca tcatcaggct gtgctgttgt gagttgcgca gaagtcagtt ttgtgaagag
    14941 cgttggtttc tctgccggta acacggttga cggcacatca gtcactttgg gcgtattgtc
    15001 aaaacccggc aacatcgcaa aaagggcgct caggcttgcg gtgacgtctt cattcagatc
    15061 atccgctttt tcgtcttttg tcgtattttt gtcagccacc gcagccaacg ccatcgtctg
    15121 agcggtggtt aacggtgttg atgtggattg ttcgtcgttg atgacaggcg gtgtttcatc
    15181 cacagggatc agtaaattag cttgttgcgc gtcggaaaca atatcgctga tcagcggctc
    15241 gccttttgtc gtgggcttat ctgttgccac cagcaactgg ggggccgctt tgtcggtagt
    15301 tgtctcgcct gctaatgctt cgctcaacaa cgcgagaaaa tcttgtgcag catcgctggc
    15361 tttgccgcca ggcaatgtgg tggtgtcaac gtcggcggta atcaagggcg ctaagcgaat
    15421 cattcaggtt tcctcatggc ggcgcgctgg gcgaactcat ccatcttttt ctgatcgagg
    15481 cggttttctg caagcagtgc cgccgtggat tgccgttcct gcagtgtctg ccaggcctgc
    15541 aaacgttgtt tcttttctcg ccagctgttc agggcaatgt caactttctg cgtccactga
    15601 ttaagttgct ggcgatgctg agtaatggct ttttccagcg tctggataaa ctgctgatag
    15661 ttgatccagc ggttgctggt catcccggca ctcatatcgc tgttgaggtt attgcgatat
    15721 tcattctgat aatcgatcag cattttgagc tgttcttccg cctgctgaca cccgcgacgc
    15781 atttcaccca gcaggcgcgc ggcatcctct acctcttttt ctgccagatc tttcagggtc
    15841 gccagcgcgc catgttctgc catctgccgt tatctcctgg gttatgacac tgtcgggaaa
    15901 atacgctcca gcccctggag agacgcttcc cagtccgcgc gttcaaaaat gccttgttgc
    15961 aaatagccct ccagctgcgg ccacagggcg atggctttgt cgagcatcgg atcgctgcct
    16021 ttggcatacg cgccgacgct aaccagatcg cggttacgct gaaaactcga caacagctgt
    16081 ttgaaggtgc gcactcgcgc gtaatgttgc tcactgatca acgccgtcat tgcgcggctg
    16141 atcgacgctt caatatcgat agccggatag tgcccggctt ccgccagtcg gcgagacagc
    16201 acaatgtgac cgtcgaggat cgcccgcgcg gagtctgcaa tggggtcctg ctggtcatcg
    16261 ccttcagtaa gcacggtata aaacgcggta atcgagccgc cgccgctaat gccatttccg
    16321 gcacgctcga ccagtgccgg taatttggca aacaccgacg gtggataacc tttggtggcg
    16381 ggtggttcgc caatcgccag cgcaatttca cgctgggcca tcgcgtagcg ggtgagggag
    16441 tccataatca gcaacacatg ctgaccacga tcgcgaaaat cttcggcaat gcgcgtggca
    16501 taggcggcac cttgcattcg caggagcgga gacacatccg ccggagcggc aatcaccact
    16561 gatcgtgcac gcccttcggc accgaggata ttctcaataa aatcttttac ttcgcgccca
    16621 cgttcaccaa tcaaacccac gacaatgaca tcggcgcggg tgtaacgtgc catcatcccc
    16681 agcagcacac ttttaccaac gccggacccg gcaaacagcc ccatacgctg cccacgccca
    16741 acggtaagca gggcattgat tgggcgcacg ccggtgtcca gcacatgttc aatcggtgta
    16801 cgttgcaacg ggttaaatgg cggggtaatc agcgcaccgg tttccgtcgt atcgggggag
    16861 ggcaggccat cgagcggttt accgctgccg tccagaacgc gacctaataa cgccggaccg
    16921 agcggcaact gcttgccgct ttgcagtcct tcagccgaaa tgtttttggc ataaacccgc
    16981 gcgccgggca ggacaccttc cacttcctcc agcggcatta aaaacagccg ttgaccgtta
    17041 aagccaacga cttcgctttc tacttcgtgc gtttcgctgc cgttctggcg ttcaatgaca
    17101 caggttgcgc cgagcggcaa ttgtaatccg gtggcttcca gcaccagccc ggtagcgcgg
    17161 gttaatcgcc cgtagcgacg taccgcaggc aactgcgcca ttttggcttc aaagttatcc
    17221 agcgtggtta gccagcgagt caggcgcgtg gtcattacac cactcctggt gctgccagac
    17281 ggcagagttc ttgccagcga gtcaggcgcg tggtcattac accactcctg gtgctgccag
    17341 acggcagagt tcttgccagc gagtggcgac actggcgtcg agatcgcctt catcggcgga
    17401 gactttacag ccgccaggat ggagggtggg atcgccccgc aagcgccagc catgcaaact
    17461 taaggtagcg ccgagcatat catccacacg ttgcagatca tccgggtgca cgcgcagctg
    17521 tggtttaccg ctgaataacg gttcttgctg caacaactgt tggatctgtt tgatcagtgc
    17581 cgagttatcc accgttggcg tctgaccgat gacctgacgt gccgcctcca gcgccatctg
    17641 catcaggcgc gacgctatca cactatcaag tgcatcaagg gtagtttgaa attcgctgac
    17701 cagttgctgc atccgggcat gaattggcgc ttgttgagac ttcgcctctg ccagaccttg
    17761 ctccagcccc tgggccagtc cttcctgata gccctgctta tgaccttgct ggcgaccttc
    17821 ggcaataccc gcctgataac cttgctcatg ggcctgcatt tgcagttgcg ccagttgctg
    17881 ctcaaggctg ggttcagcct cttcaatgat ggtttcttcc gactcgacca tgggcacaaa
    17941 ctctgcctgt ggtggcgcga gatcgtccgg cgtccaggtt ttccagggca gattatcaga
    18001 cataggtatc ctcgccgctg ccaattacca tctcgccagt ttcggcaagg cggcgcacaa
    18061 tcagcagaat cgctttctgt tcgttttcca cctgcgacag acgcaccgga ccacggttgg
    18121 cgagatcgtc gcgcagaata tcggcggcac gctgcgacat attgcgcagg aatttctcgc
    18181 gcagtggctg ctcggcccct ttcagcgcga tcaacagcga ttcggaatcc acttcctgca
    18241 acagacgctg aatgctgcga tcgtcgacat ccaccagatt ttcgaacagg aacatctcgt
    18301 cgatgatttt ctgcgccagc tcgccgtcga attcacgcac ggcggtaata acggcttctt
    18361 cctgctgagt tttcatcagg ttgataattt cggctgccgt tctcacgccg cccattttgc
    18421 tgcgcttgag attctgaccg tcgagcaagc cattcagtac ttcggtcagt tccgccagcg
    18481 cggctggctg cacgccgcca aaggtggcga tacgcaacat cacgtcgtgg cgcagacgtt
    18541 catcgaacaa cgccagaata tcggcggcct gggcgcgctt cagatgcacc agaatggtgg
    18601 cgataatttg cggatgctca tcacgaatca gatcggcggc gctctgcggc tccataaagt
    18661 tgagcgtttc aataccgctg gcggtatcgc gagtttcgag aatatcttcc agcaggctgg
    18721 cggcacgttc ctcacccaga gctttgacca ataccgaacg cagataatcg ttggcgttga
    18781 tattcagtgc ggcaaactgt tcagcttctt gctcaaactc cgccagcaca tcggttagct
    18841 gcttgttgga gatctgcgtg acgttcgcca ttgcagcgct cagggtttgc acctcgcgct
    18901 gggagaggtg cttgaacact tctgccgccc ggtcttcgcc aatggtcatc agcaggatga
    18961 cgcttttatc ggtgcctgtc aggttactca tgatcgttat ttatccactg gcgaatgacc
    19021 agcgccacca cgcgcggatc gttatcagac atttcacgga tacgctggct catgacttct
    19081 gcccccagac gttggttagc gcgccgttgt tggagttgtt cgtctttgct caggcggact
    19141 tccaccgcat cttccacttc ctcgcgggcc tgcgcctgtt gctgtacagc tttcatcgcc
    19201 tcagcgcgac gtgttagctg cgggcgtacc gctttacgcc acagcagcca cgccaccagc
    19261 agtaccagca accagcgacc ggcagccagt aactgatcga taaacgcttg ctgttgccag
    19321 aacggcagtt ctccgccgct ttcgtcacta ctattgaacg gcgagttaac gacattgagc
    19381 gagtcaccgc gtttttcaga aaagcccatc gcctcgcggg tcagatcttc aatttgtttc
    19441 atctgttcgt tgctgagagg caatggttta ccgtctggca aggttttgta attcaccact
    19501 accgcgactg acagacgttg cacatcgccc acgttcattt tggtatgacg aatagtgcga
    19561 tcgacttcgt agttactggt ttcattccgc tgtgtgctac gcggcccact attgctggtg
    19621 gtgctcgcct gctgctggcg gttattttga tttgccggag gcgtgctgat tggcgcgtta
    19681 ttcgcaggag ccggttgatt cgacaacgcc cccggtacgc cgcccggata accggaaccg
    19741 ctttgctcgc tctcattaag ctggcgtgaa cgaagcgccg cctgagattc atcaccatta
    19801 gggcgatact gttcttccgt ttgttcttta ctggcgaagt ccagttgcgc cgtgacctgg
    19861 gcgtgaatat taccgttacc aacaataggc gacaggattg cttcaatacg ccgctgaata
    19921 cggccttcga catcgctggc atatttcaat tgagcgtcat taagatcgcg cccgctggta
    19981 ttggactggg ttaacagatg tccgccctga tccaccagcg tgacgtttcc cggcggcaga
    20041 ccagcaacgg cgctggaaac cagatgcaca atggcgctaa tttgcccttc atcgagtgcg
    20101 cggccgggta acagatttac cgtcaccgat gcagaagggg atttttgttc acggacgaat
    20161 aaagacggtt tcggcattgc cagatgtacg cgcgcccctt ttaccgggcc gatagtttcg
    20221 atggtacgag aaagctcgcc ttccagcgcc cgctgataat tcacctgttc gctgaactgg
    20281 ctgataccaa acttttcctg atcgagtagt tcgaaaccga ccgcgccgcc tttgggcaaa
    20341 ccttgttgcg ccaggcgcag gcgcagctcg tgaactttat ctgccggaac ttcaatagcg
    20401 ccgctggctt cgctgaagcg gtaaggaata ttcatttgcg tcagttggct gacaattgcg
    20461 ccaccatcct gatcggaaag attgctgaat aatgtgcggt agtcgggggc tttcgcccac
    20521 aggatcagtg cgaccatgac cgccactgcc gcggaaccgg caacaatcaa tggaattttc
    20581 ggattcgcac gcaggcgatt aagccactca agagattttg tctgggctgc agtcgcattc
    20641 atcgcgcacc tcgtggctga ttatggacgg cgttattggc aaagtggaac aagactcact
    20701 cccgggtcag caaactcgaa aaattgacgc tcccattatt tgtgctttcg tttttttcta
    20761 tccgccaata aacccgtttt tttgttgcta tttagcgcct ttgtcttatt gacttactgg
    20821 taggctttgc taccagaaat tatccaggag atgaaaatgt cagcgataca ggggattgaa
    20881 ggggttatca gccagttaca ggctacggcg atgagtgcgc gtgcgcagga atcactgccg
    20941 caaccgacca ttagttttgc cgggcagctg cacgccgcgc tcgatcgcat aagcgataca
    21001 caaacagccg cccgcacgca ggcagaaaaa ttcactctcg gtgaacccgg cgtggcgtta
    21061 aacgatgtga tgaccgatat gcaaaaagcc tcagtttcta tgcaaatggg gattcaggtg
    21121 cgtaataagc tggtggcggc atatcaggaa gtaatgagca tgcaggtata ggttgtgtaa
    21181 cctgttgtta atgacatccg atctcaccgc gtggggcatg gatggggcaa acttactcat
    21241 tatctggttt gaaatggata cctgatcctg aaagttttct gccatccacg atccgtatac
    21301 acgataaacc ccctgaacat cagagtgtct catttgacac atcaccacgt cggatgttgt
    21361 tacgaccaca gacgcaacag taacctctgg ataattttat ctttgttgat gtttttattc
    21421 atgttccggg ctatggatat gagcgatttt tgatagtggt aattttattg tgttttagaa
    21481 tgcgccctgg gcaatataat ttattaaaca ggagtgtaaa tgtttccatt aaatgatctg
    21541 tcgttaaaaa ctcagtcggt tcaattaaat aaaatcacat cgaatactga gtctacgata
    21601 aaacaacacg agttagtatc tgatgatgca atcataaatg aattatcaag tgagttagtc
    21661 agttgtcttg gaaatgataa gtttacacca gttagtgaag
//
LOCUS       DAZGXS010000075        21348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGXS010000075 DAZGXS010000000
VERSION     DAZGXS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..620
                     /locus_tag="ACHKG5_002941"
     CDS             <1..620
                     /locus_tag="ACHKG5_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869007.1"
                     /translation="RYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKRSS
                     RSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGPTL
                     VTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQTMSD
                     GKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            743..1369
                     /locus_tag="ACHKG5_002942"
     CDS             743..1369
                     /locus_tag="ACHKG5_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869008.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1408..1653)
                     /gene="zapB"
                     /locus_tag="ACHKG5_002943"
     CDS             complement(1408..1653)
                     /gene="zapB"
                     /locus_tag="ACHKG5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0869009.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2079..2924
                     /gene="glpF"
                     /locus_tag="ACHKG5_002944"
     CDS             2079..2924
                     /gene="glpF"
                     /locus_tag="ACHKG5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0869010.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2947..4455
                     /gene="glpK"
                     /locus_tag="ACHKG5_002945"
     CDS             2947..4455
                     /gene="glpK"
                     /locus_tag="ACHKG5_002945"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP0869011.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4685..5695
                     /gene="glpX"
                     /locus_tag="ACHKG5_002946"
     CDS             4685..5695
                     /gene="glpX"
                     /locus_tag="ACHKG5_002946"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0869012.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5792..6538
                     /gene="fpr"
                     /locus_tag="ACHKG5_002947"
     CDS             5792..6538
                     /gene="fpr"
                     /locus_tag="ACHKG5_002947"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0869013.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6543..6971)
                     /gene="uspD"
                     /locus_tag="ACHKG5_002948"
     CDS             complement(6543..6971)
                     /gene="uspD"
                     /locus_tag="ACHKG5_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0869014.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6998..7297)
                     /gene="yiiS"
                     /locus_tag="ACHKG5_002949"
     CDS             complement(6998..7297)
                     /gene="yiiS"
                     /locus_tag="ACHKG5_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0869015.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7509..7949)
                     /gene="yiiR"
                     /locus_tag="ACHKG5_002950"
     CDS             complement(7509..7949)
                     /gene="yiiR"
                     /locus_tag="ACHKG5_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0869016.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8050..8649
                     /gene="yiiQ"
                     /locus_tag="ACHKG5_002951"
     CDS             8050..8649
                     /gene="yiiQ"
                     /locus_tag="ACHKG5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0869017.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8757..9524
                     /gene="tpiA"
                     /locus_tag="ACHKG5_002952"
     CDS             8757..9524
                     /gene="tpiA"
                     /locus_tag="ACHKG5_002952"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0869018.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9579..10334)
                     /gene="cdh"
                     /locus_tag="ACHKG5_002953"
     CDS             complement(9579..10334)
                     /gene="cdh"
                     /locus_tag="ACHKG5_002953"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0869019.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10441..11430)
                     /gene="sbp"
                     /locus_tag="ACHKG5_002954"
     CDS             complement(10441..11430)
                     /gene="sbp"
                     /locus_tag="ACHKG5_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0869020.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11749..12711)
                     /gene="pfkA"
                     /locus_tag="ACHKG5_002955"
     CDS             complement(11749..12711)
                     /gene="pfkA"
                     /locus_tag="ACHKG5_002955"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0869021.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12892..13794)
                     /gene="fieF"
                     /locus_tag="ACHKG5_002956"
     CDS             complement(12892..13794)
                     /gene="fieF"
                     /locus_tag="ACHKG5_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0869022.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(14031..14249)
                     /gene="ogrK"
                     /locus_tag="ACHKG5_002957"
     CDS             complement(14031..14249)
                     /gene="ogrK"
                     /locus_tag="ACHKG5_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFP0869023.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(14331..15494)
                     /locus_tag="ACHKG5_002958"
     CDS             complement(14331..15494)
                     /locus_tag="ACHKG5_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0869024.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIATRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGRGVTASGKKIPQVIITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDEE"
     gene            complement(15494..15973)
                     /locus_tag="ACHKG5_002959"
     CDS             complement(15494..15973)
                     /locus_tag="ACHKG5_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0869025.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(15988..18435)
                     /locus_tag="ACHKG5_002960"
     CDS             complement(15988..18435)
                     /locus_tag="ACHKG5_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0869026.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRDTQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGQELKKARQEAAALAVQFKNTERPTNAQAKAMEAARK
                     NASELQAKYNSLRLSVQRQRQELSQAGINTRNLAHDERGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVAKTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALTGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(18428..18583)
                     /locus_tag="ACHKG5_002961"
     CDS             complement(18428..18583)
                     /locus_tag="ACHKG5_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0869027.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(18580..18855)
                     /locus_tag="ACHKG5_002962"
     CDS             complement(18580..18855)
                     /locus_tag="ACHKG5_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0869028.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(18912..19430)
                     /locus_tag="ACHKG5_002963"
     CDS             complement(18912..19430)
                     /locus_tag="ACHKG5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0869029.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(19443..20633)
                     /locus_tag="ACHKG5_002964"
     CDS             complement(19443..20633)
                     /locus_tag="ACHKG5_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP0869030.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(20693..21286)
                     /locus_tag="ACHKG5_002965"
     CDS             complement(20693..21286)
                     /locus_tag="ACHKG5_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0869031.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTASEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
BASE COUNT         5101 a         5719 c         5249 g         5279 t
ORIGIN      
        1 tccgctacaa atttctttta tctggcaacg cgggtgaaat gattttatat tttgctaacg
       61 tattttttaa cccacttgcc ttatcattta ttatcgccac aatcatctgt ataacgataa
      121 aaaaaagaag cagccgcagc tttattcgcg gcacttgttg gttaatggga ttatttctta
      181 tctatagcag ctatacggtg tgggaacgac acagcatatg ggattatact ttcccggagc
      241 caggtatcac cgtcacggtt ccgagcaaac agtgggttgc caatatagta aaacaaggac
      301 caaccctggt gacccgggat gcccatgcta ttttagctat tgttgccttc tcacaaaatg
      361 aactcggtgt gcattcaatc gaggaactta ccgcgactca aaacggaact gcagaaatgc
      421 atgtttgtga tgttcaggga tttgcctgcg cttatcagga aggagtgcaa acaatgagtg
      481 atggcaaagt aagacatgct attttcatga ctctactgga taacactaat ttgatacaac
      541 tggtcgcagc tatcgaccca gattatttgg acgaatatca agaagaagta atgaaaatga
      601 tgctttctgc tcgtcaatga tctgatgtgc atgttaataa taatggattg atatgagata
      661 cgttatctta ttaatactat ggataataac tgtttttctt agtcatcttc agcacagtaa
      721 cttgcttact ggagatccag ggatggttgg cgagtatatc ggttcagtac ttcttggacc
      781 gttattactt cccgtgcttg tgtcaggtat tctgtgcaca ttcactaaaa aaacgcgtaa
      841 cttcgccagt tttactcgcg ggtgctgttg ggtattggga gtcctgcttt tatccaacgt
      901 cggcaatacg tttcgtctgt tcaccccgtg gcactatacg tttgagaagg cagctatttc
      961 cgtgactgtc ccaaaccgac actggaatac ggtttctatt agtactgata aaaccattga
     1021 tataagaagt gaagataata gcgtctttat ttctgctttc cgcctgcccg caggacgtag
     1081 cgccgatgac tcgctggaag agttaaagaa gatgcagcga gacaatctca aggatcaata
     1141 caatgaagaa accttccagt ttcatgattg taatgccaaa catttcacct gtaagtatca
     1201 ggatgtgttg ataaattttg atggtcagca gaaaagaacc atcagtgttt atcttgaaga
     1261 cacccccagg gctgtgggga tcattgcatt aatggagcca gatacggcag ataaatatcg
     1321 ccaacaggcg atggagatta tgttgtctgc taaaaataca gtgaagtaaa aaataaggcg
     1381 ccttgcggcg cctctgcgtg gaagagatta aacctcttcc atgcgaccca gcagggcctg
     1441 cagacgttcc tgccagccgt tctgctgttc tttcagatgg ttgttctcac gctccagctc
     1501 ttcgcgctga tgctgggcat tttgaacttc ctgcgacaat gagttgtttt tttctttcag
     1561 ctcttcgatt tccatctgca acagagtgat ggtatcaatc gcctgctgta cttttgcttc
     1621 cagtttctca aacacttcta atgacattgt catacctctc ctgaattgca aggcgttgat
     1681 ggataaaaaa tcctcgtccc gattaccggt gacgccttaa taaatacgag cgcactttag
     1741 ttagctccga ttgtatgaag ccgcgccatc gctgtccagc ggcacgcctt gcagattacg
     1801 gtttgccaca cttttcatcc ttctcctggt gacataatcc acaccaatcg aaaatgttaa
     1861 taaatttgtt gcgcgaatgt tctaacaaac atgcatcatg tacaatcaga tggaataaat
     1921 ggcgcgataa cgctcatttt atgacgaagc acacacattt taagttcgat atttctcgtt
     1981 tttgctcgtt aacgataagt ttacagcatg cctacaagca tcgtggaggt ccgtgacttt
     2041 cacgcataca acaaacatta actcttcagg atccgattat gagtcaaaca tcaaccttga
     2101 aaggccagtg cattgctgaa ttcctcggta ccgggttgtt gatttttttc ggtgtgggtt
     2161 gcgttgcagc actaaaagtc gctggtgcgt cttttggtca gtgggaaatc agtgtcattt
     2221 ggggactggg ggtggcaatg gccatctacc tgaccgcagg ggtttccggc gcgcatctta
     2281 atcccgctgt taccattgca ttgtggctgt ttgcctgttt cgacaagcgc aaagttattc
     2341 cttttatcgt ttcacaagtt gccggcgctt tctgtgctgc ggctttagtt tacgggcttt
     2401 actacaattt atttttcgac ttcgagcaga ctcatcacat tgttcgcggc agcgttgaaa
     2461 gtgttgatct ggcaggcact ttctctactt accctaatcc tcatatcaat tttgtgcagg
     2521 ctttcgcagt tgagatggtg attaccgcta ttctgatggg gctgatcctg gcgttaacgg
     2581 acgatggcaa cggtgtacca cgcggccctt tggctccctt gctgattggt ctactgattg
     2641 cggtcattgg cgcatctatg ggcccattga cgggttttgc catgaaccca gcgcgtgact
     2701 tcggtccgaa agtctttgcc tggctggcgg gctggggcaa tgtcgccttt accggcggca
     2761 gagacattcc ttacttcctg gtgccgcttt ttggccctat cgttggcgcg attgtaggtg
     2821 catttgccta ccgcaaactg attggtcgcc atttgccttg cgatatctgt gttgtggaag
     2881 aaaaggagac cacaactcct tcagaacaaa aagcttcgct gtaatatgac tacgggacaa
     2941 ttaaacatga ctgaaaaaaa atatatcgtt gcgctcgacc agggcaccac cagctcccgc
     3001 gcggtcgtaa tggatcacga tgccaatatc attagcgtgt cgcagcgcga atttgagcaa
     3061 atctacccaa aaccaggctg ggtagaacac gacccaatgg aaatctgggc cacccaaagc
     3121 tccacgctgg tagaagtgct ggcgaaagcc gatatcagtt ccgatcaaat tgcagctatc
     3181 ggtattacga accagcgtga aaccactatt gtctgggaaa aagaaaccgg caagcctatc
     3241 tataacgcca ttgtctggca gtgccgtcgt accgcagaaa tctgcgagca tttaaaacgt
     3301 gacggtttag aagattatat ccgcagcaat accggtctgg tgattgaccc gtacttttct
     3361 ggcaccaaag tgaagtggat cctcgaccat gtggaaggct ctcgcgagcg tgcgcgtcgt
     3421 ggtgaattgc tgtttggtac ggttgatacg tggcttatct ggaaaatgac tcagggccgt
     3481 gtccatgtga ccgattacac caacgcctct cgtaccatgt tgttcaacat ccataccctg
     3541 gactgggacg acaaaatgct ggaagtgctg gatattccgc gcgagatgct gccagaagtg
     3601 cgtcgttctt ccgaagtgta cggtcagact aacattggcg gcaaaggcgg cacgcgtatt
     3661 ccaatctccg ggatcgccgg tgaccagcag gccgcgctgt ttggtcagtt gtgcgtgaaa
     3721 gaagggatgg cgaagaacac ctatggcact ggctgcttta tgctgatgaa cactggcgag
     3781 aaagcggtga aatcagaaaa cggcctactg accaccatcg cctgcggccc gactggcgaa
     3841 gtgaactatg cgttggaagg tgcggtgttt atggcaggcg catccattca gtggctgcgc
     3901 gatgagatga agttgattaa cgacgcctac gattccgaat atttcgccac caaagtgcaa
     3961 aacaccaatg gtgtgtatgt ggttccggca tttaccgggc tgggtgcgcc gtactgggac
     4021 ccgtatgcgc gcggggcgat tttcggtctg actcgtgggg tgaacgctaa ccacattata
     4081 cgcgcgacgc tggagtctat tgcttatcag acgcgtgacg tgctggaagc gatgcaggcc
     4141 gactctggta tccgtctgca cgccctgcgc gtggatggtg gcgcagtagc aaacaatttc
     4201 ctgatgcagt tccagtctga tattctcggc acccgcgttg agcgcccgga agttcgcgaa
     4261 gtcaccgcat tgggtgcggc ctatctcgca ggcctggcgg ttggcttctg gcagaacctc
     4321 gacgagctgc aagagaaagc ggtgattgag cgcgagttcc gtccaggcat cgaaaccact
     4381 gagcgtaatt accgttacgc aggctggaaa aaagcggtca aacgcgcgat ggcgtgggaa
     4441 gaacacgacg agtaatgtca ggcacctgaa tcgattgcct gatgctgatc gcctgatgcg
     4501 acgcttacgc gtcttatcag gcctacgagc caatgccatt ttgtgtaggc gggataaggc
     4561 gttcacgccg catccggcat cacgcagact ctgaaaccgt gcgggggcaa ccccgcacac
     4621 atcaataatc ccacccttcc cctgtgctac acttcgcgcc attccttact gcttagagtt
     4681 tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc
     4741 tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc
     4801 catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg gtgaaggtga
     4861 aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc
     4921 ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg gtcaggctaa
     4981 ctcgctggcg gtgctggcag taggcgataa aggctgtttc ctcaacgcgc cagatatgta
     5041 tatggagaag ctgatcgtcg gaccgggagc caaaggcacc attgatctga acctgccgct
     5101 ggcggataac ctgcgcaatg tcgcggcggc gctcggcaaa ccgttgagcg aactgacggt
     5161 aacgattctg gctaaaccac gccacgatgc cgttatcgct gaaatgcagc aactcggcgt
     5221 acgcgtattt gctattccgg atggcgacgt tgcggcctca attctcacct gtatgccaga
     5281 cagcgaagtt gacgtgctgt acggaattgg tggcgcgccg gaaggcgtgg tttctgcggc
     5341 ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa
     5401 aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat
     5461 gggcatcgaa tccggtaaag tattgcgtct gggcgatatg gcgcgcagcg ataacgtcat
     5521 cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa
     5581 tatcgcgact accgaaacgc tgctgatccg cggcaaatca cgcaccattc gccgcattca
     5641 gtctatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat
     5701 ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg agaacgaaga
     5761 taaggcaatt caatcaaaac aggagaaaaa catggctgat tgggtaacag gcaaagtcac
     5821 taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc ctgtgcatcc
     5881 gtttaccgcc gggcaattta ccaagcttgg ccttgaaatc gacggcgaac gcgtccaacg
     5941 cgcctactcc tatgttaact cgcccgataa tcccgatctg gagttttacc tggtcaccgt
     6001 ccccgatggc aaattaagcc cacgattggc ggcactgaaa ccgggcgatg aagtgcaggt
     6061 ggttagcgaa gcagctggct tctttgttct ggatgaagta ccagattgcg aaacgctatg
     6121 gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac taggtaaaga
     6181 tttagatcgc ttcaaaaatc tggtcctggt tcacgccgca cgttatgccg ccgacttaag
     6241 ctatttgcca ctgatgcaag aactggaaaa acgctacgaa ggcaaactgc gcattcagac
     6301 ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga cggataccgg cattaattga
     6361 aagtggtgaa ctggaaagcg cgattggcct gccgatgaat aaagaaacca gccatgtgat
     6421 gctgtgcggc aatccacaga tggtgcgcga tacgcaacag ttgctgaaag agacccggca
     6481 gatgacgaaa catttacgtc gccgaccggg tcacatgaca gcggagcatt actggtaagc
     6541 ggttacttat cgataaacgg cacgatgagc aaatccgcac tcaacttatt gatcatcccg
     6601 cgatatgccg gcatcaaacg gttgataaat gagtggtgat gaccacagac aaggaggtcg
     6661 cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg ttcaatacgc
     6721 agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt atccgactta
     6781 ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg atacaactcg
     6841 cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct ggcgagctcc
     6901 aaggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac gccaatgtgt
     6961 ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa cgcagtgata
     7021 actcaaaaat aatcatctcg gcctggcatg agaaggtgaa ggccgcatca agctcaactt
     7081 gcccctccag atccttaatc ttataggcca cgctggcccc ttcatctgca gaggatgcag
     7141 cggcggcaag ctctttcagt ttggtcataa aggactctgc ttcttcgcgg gtggcaaaaa
     7201 agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac
     7261 atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt atattcgcct
     7321 ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac aagtaatgat
     7381 cttatggatt accatttttt ttgatattgc gtaaggtgaa gagttcagaa ggattttccg
     7441 ggtattgtca tttgcatcgt ggaaataaca atcccggaaa aaaatagcag taagagatat
     7501 attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg agtatcttta
     7561 ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag catcatcacc
     7621 agaatcaacg tcgggacaaa acgccctacc gcccattgcc agacacccgg taaaatcgcc
     7681 cagttacccg ccagtagcat ccacgccaca atcatcagaa atgcccatgc gccggaacgc
     7741 ccgcgatcat gcaagcgctt aacggttact gccgctgttg gccagagcaa gcacacaagg
     7801 caaaacgccg cggtctgaat atcgagtaaa tttttacccg ccagtgagaa aagcaccagc
     7861 atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat acgcccttta
     7921 aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc ttttccagga
     7981 ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc ataacaaaag
     8041 gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc attaaccgtt
     8101 acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta tctgctggct
     8161 ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa cagccagaat
     8221 cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa agccaatctc
     8281 actcgtgctg ccagtaaaat taacgagaac ttgtatgctt ctacagcgct ggagcgcggt
     8341 accttaaaaa tcaaaagcat tcaaatgaca tggctaccca tccaggggcc agagcaaaaa
     8401 gccgcgaaag cgaaagctca ggaatacatg gcagcggtga tccgcacact caccccatta
     8461 atgaccaaaa cacaaagcca gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa
     8521 cgttattaca ccgagacaga aggtgcactg cgctatgttg tcgcggacaa cggcgaaaag
     8581 gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct tgaaggtttg
     8641 aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt tcacagacct
     8701 gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga attaaaatgc
     8761 gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg gttcacgagc
     8821 tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac
     8881 caccggaaat gtacatcgat atggcgaagc gtgaagctga aggcagccac atcatgctgg
     8941 gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc tctgctgcta
     9001 tgctgaaaga catcggcgca cagtacatca tcatcggtca ctctgaacgt cgtacttacc
     9061 acaaagaatc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag cagggcctga
     9121 ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag
     9181 tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca ttcgaaggtg
     9241 cggttatcgc ttacgaacct gtatgggcaa tcggtactgg caaatctgca actccggctc
     9301 aggcacaggc tgttcacaaa ttcattcgtg accacatcgc taaagttgac gctaacatcg
     9361 ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct gcagaactgt
     9421 ttgctcagcc agacatcgac ggcgcgctgg ttggcggtgc gtctctgaaa gctgacgctt
     9481 tcgcagtaat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg
     9541 atttcatatc cggcacttac tttccttaac tcttcgcctt aacgcaaaat ctcacactga
     9601 tgatcctgaa tttcctcggc tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc
     9661 agcaatacaa atgagttatc actctgccgt accatcgcca gcccgtagct tcccatatgt
     9721 tcccgcgcct caggtacttc ttctgccagc atcataaatg ggctgcgttg taccagttcg
     9781 ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg cagtggcaac
     9841 cagcggctgc tgatgttcgc cagattgtta tccagctgtt cgcgcacatc aggacgaata
     9901 caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa
     9961 gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc acgcgcctgc
    10021 caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact ttcagtaccg
    10081 ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc ttttaaaacc
    10141 acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt ttgctgctga
    10201 ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc accggttaat
    10261 ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa aaaaagaaga
    10321 cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc
    10381 gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat cctggggaaa
    10441 tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt tttgcgcttt
    10501 cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa acgcattttc
    10561 gtactttttc gccacctcag cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg
    10621 accttctggc gagtagagat atttcaggta ggcttccgcc acctctttag tacctttttt
    10681 ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact cactcggcgt
    10741 gacgatttcg aatttatctt tccccagttc attcgctgcc agcagagctt cgttttccca
    10801 ggaaatcagt acatcgccaa ttccgcgctc gacaaaagtg ttagtggagc cgcgcgcgcc
    10861 agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg cttttgcctg
    10921 atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc agcgcgcgcc
    10981 accagagctt ttcggattag gcgtgatcac cgaaacaccc agtttaatca gatcgttcca
    11041 gtcatggatc tgcttcggat ttcccttacg taccaggaaa acaatggtgg aagtgtacgg
    11101 tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc gttccgcaat
    11161 cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac cgttgattac
    11221 cgacgttgct tgtttgcctg aaccaccgtg tgactgacga atcaccacgt tatcgccagt
    11281 ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg
    11341 atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg ccgccagcaa
    11401 aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg tgttgtgatg
    11461 agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt ggctataact
    11521 tgcgggtata tgttgggggt taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag
    11581 tgcgctttat cgatgccgga tgcggcgcga acgccttatc cggcctacaa aagtttgtaa
    11641 attcaataaa ttgtagaatt cacgtaggcc tgataagcga agcgcatcag gcatttttgc
    11701 ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt agtacagttt
    11761 tttcgcgcag tccagccagt cgcctttgaa cggacgcttc atgttttcga tagcgtcgat
    11821 gatgtcgtgg tgaaccagct gttcgttctg tatacctacg caacgaccgc cgtaacctgc
    11881 cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt cgtaaggcac
    11941 cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac gaccggtttc
    12001 tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa tcgccacgat
    12061 cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt cttcacggct
    12121 gaattcaact tccggaacca caacgaattc acagccaccg gcaatggccg cagccagcgt
    12181 cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt gagaagaaga
    12241 ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga agaaaccgat
    12301 agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcaggccga tgcacgggaa
    12361 gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga taaccaccag
    12421 cgcatcgatc ccacgttttt tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg
    12481 gaattccggg aaacgcgcag aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac
    12541 gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc cgtcataaat
    12601 gcccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt
    12661 catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt ttcttaatca tgactacctc
    12721 tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat
    12781 ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca ggtcaggcca
    12841 aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg attatgaaag
    12901 catagaccgt ttaccctccc taggtacgac ggaacagggg tcctgatgga taatcacatc
    12961 cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca tatgtgcctg
    13021 aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg tcggccctga
    13081 ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca cgatatcaat
    13141 aatttcttgc cgttcctcat caggtaatgc gcgatccagt aatgactgta ccgcctcata
    13201 tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa acagagcatc
    13261 ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc agaattgcgc cgttcatcat
    13321 aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac
    13381 cacccaacgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa ttgtcacgat
    13441 aaccccgacg cctggatctg tcatcggtgt tggagatatc agatgttgaa tgcctgtcaa
    13501 aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg cgagggactc
    13561 agctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat atcgcaccac
    13621 caataaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg cggcgagaat
    13681 actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca gtgaagccat
    13741 cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat tcataaatac
    13801 tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc attaagcagc
    13861 aggcaaattg aggataaaaa aaacccccac atcatgtggg ggaagacagg gatggtgtca
    13921 caaaaaagca cctaacctga tgatataaat ggatttatat caagcactgt ccacatagcg
    13981 tccacatcga ccataaataa agcccctcaa ctgaggggct atttttgtga tcacatccac
    14041 ataatttgct gccctgacgg caacgggtgc ggcctcacgg cgtggacttc tcccggcttc
    14101 acgatgtatc gctgtaccga ctcataagtg atgaacgtgg cgctgcaatt cacgttctga
    14161 cactggtgat aacgctcttt tgtcgtgtca gtgatatagc gacttgtacg cgcatgtgcg
    14221 gcatgctggc ataaaggaca atgaaacatc gcgagcacct cttccggttt tgttgatggt
    14281 gccattttag ttaatttatt cttacaaaac aaacagataa ataaaacaca tcattcttca
    14341 tcttctgttt cgtactccac atcagaaagc ctgacctcaa gctccaagga cgtcgtgaag
    14401 ccgctattat tcagaaaatg tgtcacctta gtgattgtcc agtcctgctc gtctatgacg
    14461 cgcttaaagc ctgacacttt gaccggcgtt tccgtgtaaa tatctgcacg accggtagcc
    14521 aggctgatgg agaactccgc aacgccccgt tgcagcttat cccacttcgc ctgagcggcg
    14581 cgcatggcct gcgctttcgt ggcataaacc gtggtcaggg caaaaacgtt gtcagcctca
    14641 ccggccatgt attcaccttc gcgcgcttcc ggtacttttg gcgctttctt ctgcgtgacc
    14701 ggtttcgctt tggggtgctc cagtgcgcgc aggtgttttt ctttcttttt gcgtttcagt
    14761 tttaccttct gcttttgagg cttcgggtct ttagtgtgta gccattttgc cgttacaccg
    14821 gtgtaggctc cacggtcagc aatcgcaaaa tgatgacggt cgccgtcgct gcgggtgatg
    14881 atgacctgcg ggattttctt accgctggcc gtcacccccc gccccgcctt aagaaataac
    14941 agttttccca tttttaccga cacctcaccg ccgttgcgtt cagcaaggcg ggtcaggaat
    15001 tttgcatcag actcctgcga ctggtcgatg tgcgggattt taattccggc cagcgacgga
    15061 gcgacactgg cttccagcct gttacgggtg gctatcgcct caacaatcgc accaagcgtg
    15121 gtgtcatgcc aggagccttc ccggcgggaa ttgagcgtcc cgcgaaaatc tgcactccgg
    15181 gcgcggatgg tgaccacatc cggcgcgccc cggtgttcaa cctcatcaac ggtaaatttc
    15241 cctttgcata ccagggcaaa acctttccag ccgatataca ccgtcaggac agcgccacga
    15301 accggcagtc cgacctgccc gtcggcatcg ttcagttcaa tatcaagctg gtcagcctca
    15361 aagccccggt tatccgtcag agtcatgctc atcagacggt cgctgatatt gccggtaata
    15421 tccctgctgt cgagcatcag catgtaatcc ggcgtcagcg tactgcctgc atcaaatgtc
    15481 agcgcatcca gcattatccc gcccccgtca tacccgtgaa tttagtcgcc atactgccag
    15541 ccttaccgat gagcgattcc gcctgtttac cgatatcgcc ataaagcgcg gccagtgatt
    15601 catcaacgcg ggtgagcgac agcgtaaaat caatttttcg gggtgtgccg tctgcaaaga
    15661 aaatactccc tgtttcactc accttgctga tgacatacat gccgtaaatc atgccggtac
    15721 catccagcaa cggccacgcc cggccttcct ctgccatcag tctgagcgtg gtcatcgtca
    15781 gcttgccgcc ggtcagttcg ggataaagca caccggcaag cgtcatgttt tcctcaccaa
    15841 cgccgagaaa ctggtaggca tcccgtttac cgatacggga atttgacggc cagcgataat
    15901 ctgattcacg ctgcatggtc tggtgtggca gcgtctggcg cataaaaaca aacataccta
    15961 acgcgagcat catttttcgt cacctcctta accgtcatgc atcatgctgg cacgggcgcg
    16021 cgcacgttta tcccgctcgt atttttcgag cgcatcctgt aactggcggt caagctgtgt
    16081 ccccggtgca gtaccacccg tcaggctgat gtgatattcg tttttactct ggtccacata
    16141 agagcggcca gccggtgccg tgaccggctg ataagcctga tagcctgcat aagagctggt
    16201 cgccggaata taaccaccgg tgccatacgt ggcggcatga gtccttgcgg cggtctggtc
    16261 aagtgtgtct gactctttgt tgataacccc gagtttttcc agtacccagt ctataccgct
    16321 gcgcagtttg ttgaacgcat taagcggcag catcagcgcg tcagccagtg cctgcccgaa
    16381 catgacgccc gtgtcacggc aacggttcag ggtgtcctgg gtggctttga ccggggcaat
    16441 caggttttta aaccactgcc acgcggcctg taacttttcg cccagccagt caaacaccgg
    16501 cttaagtggc gtgaacagtt ctcccaccgg cgcaaatgcc gctttcagcc cttccaccac
    16561 accgccaaag aatgcgctga caggctccca gtatttacgg ataagcaacg ccccggcgac
    16621 aattgcagcc accacggcca caaccggcca gctaatcgcc ccgatggccg tcataacggc
    16681 actaccaacc gtcgtgaaga ttgcccccat tgcgcctgct gccgcgatga tggcattgat
    16741 gccggtgata accggccagg ctacgaggcc aatggcaccg atgacaccag tcagcgccag
    16801 tgcaccaccg acaatgaggc cgatggttga cgccagtgat ttgtttttct ggatccagcc
    16861 gtcgagtttt aacacatact ttgtggccgt ctgcgtgaga ttacgcagtg cgccttcctg
    16921 ctggtcaaac aggtcagtcc ccaccgcctc ataagcggac tgaaactcct taaagtcacc
    16981 gccgaggtta tcctgcatga ttttaaccag ctcttccgtt ttaccgtccg aggctttcag
    17041 cgtggcggtc agcttatcca gttttccgct tgctgccgct gccagtaaaa cgttcgctga
    17101 tttcagggct tcctcaccaa aaatggtttt aaggtattcc cccttctgag acgttcccag
    17161 cttgtgttta tcaaagctgg cctgaatctc tttcagaatg gtgaacaacg gacgcatatt
    17221 tccctttttg tccgaggttt taacgccaag ctctttgagt gcatcccatg cttttccagt
    17281 cggtgcctgt aatcgggtga caacggcgct gctacccgtc cccgccattg accccctgat
    17341 gttattgtca tgcagcacac ctgtcatggc tgctgcctgt tcaagactta cacctgccgt
    17401 cttcgcaacc gggccgaggt aagtcagtgc atcactgagt ccctgaaaat cagccgccga
    17461 cttattcatc gttgccgaca acacgtcgcc cacatggctg acatcatcat ttgacagttg
    17521 aaacgatgcc ttagtcccca gcaacagttg cgcgttttct tccatcgacc gctgattcgc
    17581 cagtgccata ttcagcgtga ccggcgttgc cgcctgaata gccgcagcat ctccacccgc
    17641 tttcgcaata ataatctgcg caccggccgc atcatccgcc gaggcggcgg tattgtcgcc
    17701 gagctggcgc gcctgtttgc gtaatgcggc catttcggtg gagtcttttg ccacacctag
    17761 cacggcctgt aattctgagt ttttctgcgc aaactcataa ccgggcatca gcagcttaac
    17821 tccggccatc gttcccgccg ccgcaatccc cacaccggca gcgcctactg aggccatatt
    17881 tccggccagt tcctttcctg cctgataacg ctgtttgact gcgttaagtt ttgcctgttg
    17941 tgcactgaca cgcgccagcg cgtcacgctg acggttaagc tgggcggtgg tttcactgat
    18001 acggtttttc agccctcgtt catcatgcgc cagattacgg gtattaatac ccgcctgact
    18061 caattcctga cgctggcgct gtaccgacaa tctcaggctg ttatatttcg cctgtaactc
    18121 agacgcattt ttacgcgcgg cttccattgc ctttgcctgt gcatttgtcg gtcgttcagt
    18181 atttttaaac tggacagcca gagctgcggc ttcctgtctg gcttttttca gttcctgacc
    18241 agtcacggcg agctgtgcac tggtcttgcg aaatccctca atacgggatg cctgaccgtt
    18301 cagctcgcgc agtgattttt gtgtgtcccg gatatccccc gacagcgact tgctcgctgt
    18361 acggatggat ttaaacgggc gggatgcctg gtcaacagcc ctgagcaata cctgtaattt
    18421 tacattgtta ctcattcgtg tttccgcttc gccggagcgc cttttcgcgc catgtgatga
    18481 gttcggtcag gctcatggga tacagttctg atggcggcca gtgaaatatc actgccacat
    18541 ccgccatcag gtcatcgacc gacagatttt tcggaaacgt cactgcaccg agttcggcga
    18601 caaaaaaccg accaccttac cggccagcgc cacaaggtca ggcagttcca gcgcggcgac
    18661 ttcctgctcg gtcagcatcg gtgccgtcat gcgcggcagc accttaatca gtgcatcgac
    18721 ttcggagttc gcgaccgcag ccagactgac accgcgcagc gtcccggcat taggtttcat
    18781 cagcgtgacc tgttcgataa cctgctcacc acgtttaacc ggattgtcca gggtaatgac
    18841 attttctttg ttcatggttt tctcacttct gaatcagggt taaccggtca gccaggctga
    18901 ccggatgaaa atcacaggcc gatattgcgg cggtgttgct ccagccggtc gacgccgttc
    18961 accttctcaa tcatgttgat ggtgtcgatt tcgaccagct ccttaccgtc catcgtcagc
    19021 cggaaatagg tgcagaccac ggagattttc gactcggtgt cttctccctg tttaccctcg
    19081 ccggtgtcga tttctttctg acgtccacgc atgaccacct cgacggccac cgtttcgccg
    19141 gtatcgtcgc gctggtaaga gcctgcaaaa cgaatcggta cggcatccac accggttgcg
    19201 gcgtaaagct cccagataac cgaatcaggg aagccaccga gcgaccactc cattgacagc
    19261 gcatcgtcat caaggccgag gtctaccggt gcgctgccat tcatccccgc accgcgatag
    19321 ttttcgagct tacgggtcag ttttggcagc gtgacggact ttgcaacgcc ctgatagctg
    19381 tagccgttca gaaagacgtt cattaacttg agtttgcgcg gcattgccat cggtcaggct
    19441 ccttaattgc tgttaaccga ggtgaccaga tttgccaggt atttatcggt gatacgctgg
    19501 cgcagggtca ggttttcaag aggaggcacc ggtgtatagt cgtagtcgat atacagtttt
    19561 ccggccttga gggtttccgc atcgttggat tcttcgctga accagcaggt cgcatccacg
    19621 atatagccgt ttgttttcag ctcacggaat ttggcattga tgccgtcaac gatgtcacga
    19681 atcagcgttg cggtgatggg cttgtccacc gcccacatgt gcgcctcagc catcgtgtcg
    19741 gccagcacct gcgcggtgcg ggtgtagttt tcaaagagga acagcgggtc atcagagcag
    19801 gtacggttac cccagaagcg gaaaccgtcg cggcgaatca gcgtagtgac gcctgactcg
    19861 ttaagcaggt cagcatcggt gccggactcc tgcaaatccc agaagacgga tgcgctgatg
    19921 ccggtaacac cgttcacccc gacattggac agcgttttat gccagccctg ctcctggtcg
    19981 attttagcgc gcagacccag cgcacgggcg gtggcatacg cggtggcggt ggtactggcg
    20041 accgtatccc atgcgaggaa atccggccag atgaccatca gctcacgctg gctgaaattc
    20101 tggcggtagg ctttcacctc ggaaatggtc ttacatcccc atgcgctgat atacccgaaa
    20161 gcacgcagct tctgacagac tgatgccagt gcaacagcca cctctttggt gtccagtccc
    20221 ggcacgccga gaatacgcgg tttaacacca gttaccgact ccgccgccag cagggctttc
    20281 agtccggtgt actgaccgtt ttcgtcggtg gtgccgatga tattggaaac ggtctgcgcg
    20341 agttttgttt cctcgtcgtc gccggtgccg tcttccacac gcacgacaac ggtgaccggt
    20401 tttgactggt cagcgatggc ctgcaacgac gccgccagcg tgcctttttt accggccttt
    20461 gcaattgcgc tttgcacatt ggtaatcagc accggtttat tgagggggaa ggtttccgca
    20521 tccgcatcgc tggccgtgca gaccatgccg acaatggcag tggatacggt ggaaatgacg
    20581 cgggtgccgt cgttaatctc cagcacctgc acgccgtgat gatagtcact catccgttta
    20641 actccgtggt taatgggtga gtggtatttt cagttgtgcc ggagatgtca ggctatttgt
    20701 cccggttggc taagggatga cacaatttat tctttgtcgc tgatgagggg aattttttat
    20761 agagcgtgga caggccaata tcaaaaatca gcgccacacg ttgacgagat tcccccgcag
    20821 ccagcaaccg tccgacctgc tcccactcac tcgcggtgag tttcggtcgt ctgccaccaa
    20881 tacgaccttt ggctctggcc gcttccagcc cggcccgagt ccgctcgaca atgagttctc
    20941 gttccatttc agccagggca cccatcacat gaaagaaaaa acgccccatc ggcgtgctgg
    21001 tatcaatagc atctgtcagg ctgcgaaaat taacgccgcg ttcgcgcagt tcctcaacca
    21061 gaatgaccag atgccgcata ctacgcccca gcctgtccag cttccagact accagagtgt
    21121 cacctgctga taatgtcctg agcagttttt tcagtcccgg cctgtcggac ttcgtgccgc
    21181 tgattttgtc ctcaaaaatc agctcacatc ctgcgcactc cagcgcatta cgctgcaatt
    21241 ccgtgttctg gtcatttgtt gacacgcgta catagccaat aagcatgagc atccccctga
    21301 ataaaaaccg gagatgatgc cagttacccg ttatctctgc attttcat
//
LOCUS       DAZGXS010000076        20873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGXS010000076 DAZGXS010000000
VERSION     DAZGXS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(16..516)
                     /locus_tag="ACHKG5_002966"
                     /pseudo
     CDS             complement(16..516)
                     /locus_tag="ACHKG5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1175..1357)
                     /locus_tag="ACHKG5_002967"
     CDS             complement(1175..1357)
                     /locus_tag="ACHKG5_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869032.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1296..3386
                     /gene="iha"
                     /locus_tag="ACHKG5_002968"
     CDS             1296..3386
                     /gene="iha"
                     /locus_tag="ACHKG5_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0869033.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3604..3763)
                     /locus_tag="ACHKG5_002969"
                     /pseudo
     CDS             complement(3604..3763)
                     /locus_tag="ACHKG5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3966..4286)
                     /locus_tag="ACHKG5_002970"
                     /pseudo
     CDS             complement(3966..4286)
                     /locus_tag="ACHKG5_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4709..5284)
                     /locus_tag="ACHKG5_002971"
     CDS             complement(4709..5284)
                     /locus_tag="ACHKG5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0869034.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5353..5931)
                     /gene="terD"
                     /locus_tag="ACHKG5_002972"
     CDS             complement(5353..5931)
                     /gene="terD"
                     /locus_tag="ACHKG5_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP0869035.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(5980..7020)
                     /gene="terC"
                     /locus_tag="ACHKG5_002973"
     CDS             complement(5980..7020)
                     /gene="terC"
                     /locus_tag="ACHKG5_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP0869036.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7043..7498)
                     /locus_tag="ACHKG5_002974"
     CDS             complement(7043..7498)
                     /locus_tag="ACHKG5_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0869037.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7521..8678)
                     /locus_tag="ACHKG5_002975"
     CDS             complement(7521..8678)
                     /locus_tag="ACHKG5_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0869038.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8678..9259)
                     /locus_tag="ACHKG5_002976"
     CDS             complement(8678..9259)
                     /locus_tag="ACHKG5_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0869039.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            9582..10640
                     /locus_tag="ACHKG5_002977"
     CDS             9582..10640
                     /locus_tag="ACHKG5_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP0869040.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            10650..11792
                     /locus_tag="ACHKG5_002978"
     CDS             10650..11792
                     /locus_tag="ACHKG5_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP0869041.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            11785..12558
                     /locus_tag="ACHKG5_002979"
     CDS             11785..12558
                     /locus_tag="ACHKG5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869042.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            12560..13639
                     /locus_tag="ACHKG5_002980"
     CDS             12560..13639
                     /locus_tag="ACHKG5_002980"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP0869043.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            13639..14595
                     /locus_tag="ACHKG5_002981"
     CDS             13639..14595
                     /locus_tag="ACHKG5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP0869044.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPSLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            14606..15814
                     /locus_tag="ACHKG5_002982"
     CDS             14606..15814
                     /locus_tag="ACHKG5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP0869045.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMAQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            15832..16299
                     /gene="terW"
                     /locus_tag="ACHKG5_002983"
     CDS             15832..16299
                     /gene="terW"
                     /locus_tag="ACHKG5_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP0869046.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            16560..16889
                     /locus_tag="ACHKG5_002984"
     CDS             16560..16889
                     /locus_tag="ACHKG5_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0869047.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            16876..17217
                     /gene="tnpB"
                     /locus_tag="ACHKG5_002985"
     CDS             16876..17217
                     /gene="tnpB"
                     /locus_tag="ACHKG5_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0869048.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            17299..18129
                     /locus_tag="ACHKG5_002986"
                     /pseudo
     CDS             17299..18129
                     /locus_tag="ACHKG5_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18160..19773)
                     /locus_tag="ACHKG5_002987"
     CDS             complement(18160..19773)
                     /locus_tag="ACHKG5_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP0869049.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(19804..20154)
                     /gene="tnpB"
                     /locus_tag="ACHKG5_002988"
     CDS             complement(19804..20154)
                     /gene="tnpB"
                     /locus_tag="ACHKG5_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0869050.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20151..20576)
                     /locus_tag="ACHKG5_002989"
     CDS             complement(20151..20576)
                     /locus_tag="ACHKG5_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0869051.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            20630..20873
                     /locus_tag="ACHKG5_002990"
                     /pseudo
     CDS             20630..20873
                     /locus_tag="ACHKG5_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5163 a         5342 c         5289 g         5079 t
ORIGIN      
        1 tggataaaga ttctaacaaa gcatttcaac aatatatact tgttaaaaat ccatatcgaa
       61 atgccgttgc agcattaata tatgcctcat tcataaaatg taaaagagca agctcgtacc
      121 agtagggggg aaattcattg acatgtcctg tcaatacgta cagagccctg ccatgcttgc
      181 cagcacgtaa caattccgcc cccagtaacc agcgtgttcc ctgattattc tcagggttat
      241 atgttagtat tttatcaatg agtataacgg catcctgatg acgctgtaaa tgaacattcg
      301 ccagaattgc agcataaaga gcgcaaatgc atatgccaga tgagcgtgaa tatcaatgat
      361 gtcaggagca tgtcaggcaa gacgcctgag tttgttgacg taatttgttt cgtttatttc
      421 atcactatcg tatgcgtcaa gagttttttc aaactcatcc cgaaatgggc caagcctggt
      481 ttcaggaaat aagaaatacc cttggttatt gttcacttaa aagtataatt tgtaattcaa
      541 atcctgagcc tgtaaagcgg ggagtaacat attctattct gaagagaata aaagtcgtga
      601 tgcgatgtat caagcccgga ttgtaatccc agattaacat agatcacaaa acaacttatt
      661 cttcactaac gtcaagataa atatcgttgt atgccttatc acgactacga ataccagcaa
      721 gatacaactg acatacggaa agataccacg ttttttactc ccgaaaataa cgctaaaaag
      781 ctacttcccc atcgtttgtc cttagtattg ccagcgccaa caatgtgggc tgacatgata
      841 aagctgtcta ggaaattgtt cgcctcctca gcggacaatc caaatggtga ttgtctctgt
      901 taaacgttta ttttgaaggt cgactgaata aggtgatgac gctgtagaat ttttcacgtg
      961 ccacagaatt ttgaacgctt tctcttacaa tatttcaatg tttctatcag tattcgccgg
     1021 aagaagtcat cgaccaaatc atcccagtcg tctcgcatca ctgaccattc atggttgaca
     1081 tgtggtggaa atccgcttct acagtaacca ttttttattc gcaaaaccga acacgccata
     1141 cggatagctg ttaactggca tgccgaggca gtcgttattt atatttggtt ttgtcaataa
     1201 tctttatttt ttgtaaaagg caaatataaa ttattctcat tattgtttgt atttgtgtat
     1261 tgtcttgccg gttaacatga tcggagatta gtaatatgcg aataaccact ctggcttccg
     1321 tagtcattcc ctgtctcgga ttttcagcca gcagcatagc tgctgcagag gatgtgatga
     1381 ttgtctcggc atccggctat gagaaaaagc tgactaacgc agccgccagt gtttctgtga
     1441 ttagccagga ggaattgcag tccagccagt accacgatct ggcggaggct ctgagatcag
     1501 tagagggtgt ggatgttgaa agtggtacgg gtaaaaccgg agggctggaa atcagcatcc
     1561 gaggaatgcc agccagttac acgctgatac tgattgatgg tgttcgtcag ggcggaagca
     1621 gtgacgtgac tcccaacggt ttttctgcca tgaataccgg gttcatgccc cctctggccg
     1681 ccattgagcg tattgaggtt atcagggggc cgatgtccac actgtatggc tctgatgcga
     1741 tgggcggtgt ggtgaatatc attaccagaa agaatgcaga caaatggctc tcttccgtca
     1801 atgcagggct gaatctgcag gaaagcaaca aatggggtaa cagcagccag tttaatttct
     1861 ggagcagtgg tccccttgtg gatgattctg tcagcctgca ggtacgcggt agcacacaac
     1921 agcgtcaggg ttcatcggtc acatcactga gcgatacagc agccacgcgt attccttatc
     1981 ccacggagtc acagaattat aatcttggtg cacgtcttga ctggaaggcg tcggagcagg
     2041 atgtgctctg gtttgatatg gataccaccc ggcagcgtta tgataaccgg gatgggcaac
     2101 tggggagtct gacgggggga tatgaccgga ccctgcgcta tgagcgaaac aaaatttcag
     2161 ctggctatga tcatactttc accttcggaa catggaaatc gtatctgaac tggaacgaga
     2221 cagaaaataa aggtcgtgag cttgtacgca gtgtactgaa gcgcgacaaa tgggggcttg
     2281 ccggtcagcc gcgggagctt aaggaatcga accttatcct gaattcatta ctgcttaccc
     2341 ctctgggaga atctcatctg gttacggtgg ggggcgagtt tcagagctcg tccatgaaag
     2401 acggagttgt ccttgccagc acaggtgaaa ctttccggca gaaaagctgg tcggtatttg
     2461 ctgaggatga gtggcatctc acggatgcac ttgcgctgac tgcgggcagc cgctatgaac
     2521 atcatgagca attcggggga cacttcagtc cgcgtgcata tctggtctgg gatgtggcag
     2581 atgcctggac gctgaaaggc ggtgtgacca cgggatataa ggcacccaga atggggcagc
     2641 tacataaagg gattagtggt gtgtccgggc agggaaaaac aaatctactt ggtaaccccg
     2701 acctgaagcc ggaagagagc gtcagttatg aggctggggt gtattacgat aaccccgccg
     2761 gtctgaatgc caatgtcaca ggttttatga ctgacttctc caacaagatt gtctcttatt
     2821 ccataaatga taacaccaat agctatgtaa acagcggaaa ggcccggttg cacggtgtgg
     2881 aatttgccgg cacattgccg ctgtggtcag aggatgtcac gctgtcactg aattacacct
     2941 ggacccgaag tgaacaacgt gatggtgata acaaaggtgc gccgctgagt tatacccctg
     3001 aacacatggt gaatgcgaaa ctgaactggc agatcaccga agaggtggca tcatggctgg
     3061 gtgcccgtta tcgcgggaaa acaccacgtt tcacccagaa ttattcgtca ctgagcgctg
     3121 tacagaagaa agtgtatgat gagaaaggag aatacctgaa agcctggacg gtggtggatg
     3181 caggtctgtc gtggaagatg acggatgccc tgacgctgaa tgctgcggtg aataacctgc
     3241 tcaacaagga ttacagtgac gtgagcctgt acagtgccgg taagagtacg ctgtatgccg
     3301 gtgattactt ccagacggga tcatcaacaa caggatatgt gatacctgag cgaaattact
     3361 ggatgtcgct gaactatcag ttctgataat aacaaaacgc tatcactgac ggtagaatac
     3421 gttgccactg caactcctgg cggaacagtg gcaacgtatt aggttaagtg catttccgat
     3481 ccgctaatga gatttcgtta ccaacaacta atatcgtcac aggaaatgca cggattattt
     3541 ttaacttatc atttacatac ttgtccagag tgtaagcgca ccgtgaagga cgtggggtaa
     3601 aaattagttt acagagagag tgacgttcca gggcaacaac tctttcatgc ggttggcagg
     3661 ccaggtgttg attacactga tcacgtggcg tagccacgtt tccggctcga ttccgttaag
     3721 ttttgcagct accgatcagg ctgtacatca ctgccgcact atcgctcgtc atctcaaagt
     3781 ccggtctcgt cagcaggaag gtatcattct ctcccgccat ttttccaggg gcccggtcag
     3841 ataagtccct ttgtctatcg ctgactcctg actcataacc cggttagcag aatgcagggt
     3901 caccacctcg ccacgaccaa atccaaataa gtcaattgca ccttctcaat cgccattttg
     3961 tcagtaagcg tacagcctga actgatggta tcttcaccat caatgacaac ggtgatcgca
     4021 attttactga cgttcgccgg aacacgatcc agtgctatct caatgctggc ctgctgcgaa
     4081 ccggtaacga gcctgactgc cccctcagga gaagacaaat tattataaaa gataaagtca
     4141 gaatcgccac tgacctttcc ctgagcatta agcatgaaca ggcaggtatc gggttcgcct
     4201 ttaaagccgg atttggcagg gtactgaaga ttcagcctga tcgtagattg ctgaaggggt
     4261 atgttttgtc cggattgtaa attcatatta actctcctga tttttgatta ttattaatgc
     4321 gcagcgttta tatatgttcc ctaggcttag ttctggacgc tggatattcg gtgaggcgta
     4381 aatatggtat gacaccattt ttcataacgc tgaagtttct atacctgttg aatttgaatt
     4441 ttcattgacc gggtatctta ttttccaggg cccgttcctt cataagtcgc aaaagtaaca
     4501 tatatccgaa gggcatgctg ttgatatcag acacggaata ctggctttaa ccagcaacca
     4561 cagataaacc cggggtctgc ataaaaggtt catgccagaa ttaacatagc tctgctttct
     4621 gccccccccc tttcccatat tcacccgttg atagcggatc gatacccaaa aaaaaccccg
     4681 gcattgccgg ggtcagactc agcgtctttt agacattgac gccgtgctgg gaagcaagcg
     4741 ctgacagacc accggcaaaa ccctggccaa cagctttaaa cttccactca gtaccatggc
     4801 gatacagttc accgaagacc attgcggttt cggttgaggc gtcttcagac agatcgaaac
     4861 gggcaatttc cgtcccgttg tcgttgttgt aaacgcgcat gaagctgttg ctcaccatgc
     4921 cgaagttttg tttacgcgct tctgcatcat agatggtaac ggcaaatacc agttttttga
     4981 tgtctgctga gactttggtc agatcgattt tgacctgctc atcgtcgccg tcgccttcac
     5041 cggtacggtt gtcgccctgg tgctctactg cgccatcagg gctggtttta ttattgaaga
     5101 aaatgaaatg ggcatctgac agtactttac cgtcttcacc tactgcgaat acggaagcgt
     5161 ccagatcaaa accctgacca tcggttacac gggcatccca gcccaggcca accatagcga
     5221 cattcatggt tggtgcttct ttggtcagag atacgttgcc gccttttacg agagaaactg
     5281 ccatttttag ctcctgcaaa cagttgaatg aggctgaatt aacagcccca gatatgaaaa
     5341 gttacttttc gatcaggacg cgttaatgcc gtactgagca catacagatg ccagaccacc
     5401 agcataaccc tggcctactg cgcggaattt ccactcacca ttgtggcgat acagctcgcc
     5461 gaacagcatg gcagtctcag tggacgcatc ttcggtcaga tcgtagcgag cgacttcagt
     5521 ctggttatcg tcattaacca gacgaataaa cgcaccggat acctgaccaa agctctggcg
     5581 acgagcctga gcatcgtgga tggtcacaac gaagatgatc ttgtcaactt cagacgggac
     5641 ggcgtccagt ttaattttca gcgattcatc atcaccatcg ccctcaccgg tgcggttatc
     5701 gccggtgtgc gttacggaac cgtcggatga cgtcaggttg ttatagaaga tgaaatctga
     5761 atcgccgcgc actttgccgt ttgaggccag caggaatgct gaagcatcca ggtcaaagtc
     5821 ctgaccgtct gttgaacgcg catcccagcc aaggcccacc aggacatttt tcattgacgg
     5881 agctgcttta ctcagggaga cgttcccgcc tttggaaaga gaaacactca taaaataacc
     5941 tcttcgatta gtaattgttc aggttaacac ttaaggggat taactcccct tttcctcaga
     6001 ttcaggtttg cccgggaaca tgacgcttgc gagaatgccc agcgccagta cacccagtac
     6061 aacatacagg ctggttgttg ccgcgatgct gtaaccatga tgccagatgt gatcggtcgc
     6121 attcaggccg agttttgccg cgatgaagaa cagcagcacg ataacggcct tctccagatg
     6181 aaccaggtac tgtttcagtg cctcaaggac aaaatacaga gtacgcagac ccaggatagc
     6241 aaacatcatg gcactataga cgataagcgg ttcacgactg acggcaatga ttgccggtac
     6301 cgagtcaaac gcgaacatta cgtccgagag ttcaaccaca gccacacaca ggaacagcgg
     6361 ggtcgcatac agcgccgctt ttttaccacg gccaatggtg acatcgctgt tttctggttt
     6421 cgccagttca gcatccactt ccttctggtt aagtaggaag gcatgccctc tgagcttcgg
     6481 ccagataggg aagaagcgtt taaccatacg gtaagccaga tgctgggagt aatcctcaat
     6541 ttcatcatcg tcatcaccgc ttttaagcat catgaccgcc gtccaggcaa cgataatagc
     6601 gaagacaact tcaacgtacg gccccagact cagcaggctc gtaccgatgg cgacaaagat
     6661 gcccctgaag acgatggcac caatgatccc ccagtagaga acacggtggc gataacgatc
     6721 cggaacggcg aaccaggaga agattgccat catgacgaac aggttatcga ccgacagcac
     6781 tttctccagc gcataacccg tgacaaacag actggcaacc tcagcaccgt ggtggatata
     6841 gaggaaaccg gcaaatgcca tcgcaaccac aacccagaat acggaccaga gcgcagcact
     6901 cttcagcgag attggcttgt catcacggtg cataaacagg tcaataaaga tggcaccgac
     6961 tgaaagcgca ataaaaacaa tgaccgtttc agtcgggaag ccgatgtgtg tggaaaccat
     7021 attttaccct tagggatgtg gatcagaggc caaattcagc cgggtcgaag cccaacgcga
     7081 tagcgatctc gcgggaggcc agtttctcgt cctgatcgaa gttaccgtca cttttcgcaa
     7141 cggcaatacc aacacgtaag gccagctgag cggcctcagg ctgatctttc agcgccagga
     7201 tgtatttcat ggtttcgccc ttgccaattt caacatcgaa atcgaagctt gaaaccagtt
     7261 tattgaagaa ctcaatcacc tcattggtat cgaagacctt cagctcttct gaagagcgca
     7321 gaaagcccat cattttctgc ttttcttccg aacttacgcc gtcactcgat acggcaatac
     7381 gggcacatac agcaacggtg ccctgcatga attttttgtt tttgaaacgg ccaacctggc
     7441 gggtcagttc gtcacggcct gagttaatgg cacctttaac tttgtcgaaa aagctcatac
     7501 ctgtttcctt tgttgctgtg ttatttagaa ccggcactcc agcgaaatcc ccagccaaat
     7561 gcccggtcca tttcgtcctg gcctttgaag tactggttaa ttcgctcgac tttaattgcg
     7621 ccgttttcgt ttacgagtct ggcaatggcg cacaatgtgc gacggttctc accttcggtc
     7681 aggcgggtct caatgggcgg ttgatccggc acatgaatgg tgactacacc atcagtcttg
     7741 tcccagctgg gaacaccttc ataaataaag gcgtaaatca gcacttcgcg gatatgcttc
     7801 cattcacgtc cattgatatg cagccactcg ccgtctgata catcacccgt ccggtcatcg
     7861 cccttgagct gaacatacgg ttcatcgcga taatcaccga atgcattacc gagcgcctga
     7921 atgaccgatt tatacccgtc ctgaagctca acaaaggccc ccagatccag gtcgattcct
     7981 ttggagccga acatacctgc aaagcctgat ttgctactgc cccggtgcca gttcaggttg
     8041 atccggattt caccgaagtt atcccgtttg gtcaggctga ttgcaggctt ctcttttgtc
     8101 agcgatacct tgctcaggct gatttttgtc tcaactggcg gaggggttac aaccggagga
     8161 gttggggctg cgggagccgg ttcgtcggca atatttacgc cgaaatgctc tgccagcggt
     8221 ttaagtccac cgttgaaccc ctgcgcaacg aagcggaatt tccagtcatt attacgacgg
     8281 taaaattccc ccagaattaa ggctgcttcc tgacggccgc tcagttcgac actgccgcta
     8341 accaggccgg tagccccttg ctccacgtcg atggaaagat tacgaagacc ggacaccgtc
     8401 tgcccaccgt cacaggtcac ggtgaacgca attttctgga catcaggttt cagccggttg
     8461 agcgcgacag taaaggttga gtactggcct tcactgacca ggctgacggt accatcatcg
     8521 ttacgtggct gaccataaaa caccatgtcc gcatcaccct gcaccttccc gtcggcatac
     8581 aggcgaaacg ccgaagcatc cacctggccg cccgaggtga tccggacccg caactcctgg
     8641 gctggaacgg gggcattgcc ccccggcgtc aggttcatta gcgaataacc gctgaaacga
     8701 tatcggagtg catatcgtca attgtgcggc cgcgacaggc atggccaagg gcggtaaagt
     8761 cccagttgcc gttattacgg cgcagcgaag aaataacgat cccggtatgt gagccctgct
     8821 cagtcagctt gtagcgagcc agctctttgc cggtttgatc gacaacgcgg cagaaagcgt
     8881 tttcgacgtc attgaacgac tgaccacgga agctgtttac cgtaaacgcc aggtattcaa
     8941 cgtttgcagg caggcgggag aggttgacct taatcacttc atcgtcaccg tcaccctcac
     9001 cggtcaggtt atcgccactg tgcacaacag cgccgcaggt ggatttcagt ttgcggaacc
     9061 agatggtgtc aattgtttta cctgtgctgt ccattaatac acagccagca tcgagatcga
     9121 ttgagtcgtt gcctccaaac aatcctccaa gaagaccttt tttcttaacc ggatcccaac
     9181 cgagaccgaa atgaagctgg cttaatgcag atgattcttt gctgagtgat accgtctggt
     9241 tcttaactaa tgaaaccatg ttttattcct taaaatggta atgaccgaga caatcaaagg
     9301 tatatttagt aactgacgaa gaaaatcata tcatgatgaa gttctctgac aagggaaatt
     9361 tttgtggttt tctgtaaaaa cacaaaaaga aatccttttc agggtgttag tttttctatt
     9421 tttaatttaa atatcaatgt gttataagga atccaaaaaa cactgacgca ctccataacc
     9481 acaggagtta ttaatcatat tatgattgac atgtagctta gacactcata gactcacttc
     9541 tcaatcataa atttgcatgg cgttccggat aattaaaaaa aatgaacaag aaaatttggt
     9601 ttatggaagg tttatcctcc cagcgagata ttattcaggg ggtaaaatca tttgcacaaa
     9661 aaaataattt tgccattacc gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt
     9721 ccgttgccga ttattctttg actgaacctg aagatcctca aaaacgtctt cagtttatcc
     9781 aggaaaccat tcagacctac ggcatccacc atattcatac tggccgtaac agccagtggt
     9841 ttgaagaaca ccgttcagcc attgaaccga ccggtgccac ccttactacc ggtgcaacgg
     9901 gcgtcgactg gttaactctg gctgacgaaa aagttacttt tgctcagttt atggagcaaa
     9961 agggtctccc ggtcgtacca tcctggcggg tgaatacgct ggcagaatta aagacacacc
    10021 tcgcggcccc gccgttcact gacagcccgg tatgcgtgaa gccggtgacg ggtatctatg
    10081 gcatgggatt ctggcgcttt gatgacagtg cttcgcctat ggccgtcttt aatcatcccg
    10141 aacatcgtct ggtcagtccg caacagtata ttgcagcagc atcagctgct gagtcgttta
    10201 aaccccttgt tttgatgccg tacctgccag gcccggaatt ttccgtcgat atcctcgcgg
    10261 ataagggcga aatactcgca gccgtgggac gccgtaagga aggggctatc cagtatctgg
    10321 taaacgaagg aagcgcctgg gaactggcgt gtgactgcgc ccgtgttatg aaggccgacg
    10381 ggctggtgaa tgttcagacg cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca
    10441 acatgcgtcc gtcagggggg gtgggttata cccttcacag cggtgtgaac cttcctgggt
    10501 tatttgctgc ctttaagctc ggtctgatgt ctgaagatat ggtacgccag agcgctaaaa
    10561 acaccttttc tccggttgcg gtgagatcca ttacggatgt aattgcatac ccggaatcac
    10621 tctctaacct tctgaattaa ggcgtattta tgtctgatat cctcgaagat atgatttatc
    10681 gccgtaccct ctcatgcggt acgattcagg taacccgtga ccagggtgaa gtttcgctcg
    10741 atgacctgtt tgacatcgct gaaagacgta acccgaaacg cgcctttctg tttgtcagca
    10801 aagtactcgg caggcacatt cccgtgccac cctcagtcat gcggcaggcc tacagacagc
    10861 ttgccagcca gttcccctcg acactgacag gacccgtact gtttatcggt atggctgaaa
    10921 ccgccgttgg gcttggggcc ggtgtatttg atgaagtgcg ccaccagcat cgtgaatctg
    10981 tctatctgac ctctacccgt cacccggttg atggcacgtt gctttgcgag ttcaaggaag
    11041 aacacagcca cgccaccgat catctgatct atctgccaga tgatgaagag aaaagacgtc
    11101 gtgttaccaa cgcacgaacg ctggttttga ttgatgacga ggcaaccacc ggtaatacct
    11161 ttattaacct gctttctgcc ctgcgtaata ccggcaagct tcaacatatt gaacaggtta
    11221 tcgccgtaac gctgaccgac tggagtggca atgccctgtc ggagcgcacc cccttgcccg
    11281 tcacttcggt ttcgcttgta agcggtcagt ggggatggac cccattacct gatgcacctg
    11341 tcccggacat gccgaaagtc aacgtaactt cacgaggaga atgggacatc caggggaaac
    11401 agtcatgggg ccgactgggg atgctcgcac ctgcggccga tctcggccat gaggtctccg
    11461 tccacaaggg ggaacgtatt ctggttctcg ggaccgggga attcgtctgg gagccgttcc
    11521 tgcttgctga acggctcgaa gctgccggag cacaggcatt ttatggatcg accacccgct
    11581 cccctgtcgc cgttggttat gccattgagt ccgccatttc ctttacggat aactacgggc
    11641 tgggcatccc caattttgtc tataacgtcg cccaccagca gtttgaccgc attcttgtgt
    11701 gtactgagac acccgcagaa agtattgaca cgcagcttct taaagcgctg gctgaggttg
    11761 cgcccgtcgt ggagattgtt acctatgaat aaacccgttg ttctgagcga ccttgacgac
    11821 acgctctttc agacccgtcg taaaatggtg gacgagctgg ccctggagcc attccgtacc
    11881 ggtgccgttg atcgcaccct gaatccacga agctttatga cggaagaaca gtccatgttg
    11941 gtggactggc tcctggagca ggctgaactc atacccgtta ccgcacgggg aactgaagaa
    12001 atcagccgcg tacggatccc tttccactcc tgggcaatca caacgcacgg ggccgtcatt
    12061 ctcacgcctg aaggcaaacc cgacgaggag tggaaggccc atatgctcgg ccagctggct
    12121 ccctaccagg aaaagctgac atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa
    12181 ggaatcaatg cctgggcaag gctgaacttc gaatacggtg aaacggcggt ttatatggtg
    12241 atgaaacacc gcgacagcac ccgccttgac gagctcaatg ccattgcaga tgagatagaa
    12301 acggtgtttc cgaccgaggg cttctacatc caccgcaaca gtaataacgt ggcctggctt
    12361 cccaccccgg ttgagaaagg gctggcagtc agctggctgc ttgaaaaact tcgggctgaa
    12421 cgaggagttt tccccgtaat tggtttgggc gacagcctga gcgatcatcg ttttatgaaa
    12481 ctgtgcagct ggtttggcat cccgcgtcag agtcagtttg cagatgccat ttcacagcga
    12541 atttttggag aaaattaaaa tgcagccaca tgaaacattt accggctcat accagcccgg
    12601 tgacgtggaa tttctgctaa agccggttgt cattgagatg acgccggttg agaaaaaaga
    12661 agaactgatt cagtccggga agaaacatta ttcggacatg ctcagccagg agccagcgcc
    12721 tacacaatgg catcttgatt tgtttcaccg ggcgctggat cggggcgcag agaggcttgc
    12781 aaaagaagtc acacagctcg ctattgctct ggccgaacgc ttcggtgatg agcccattgt
    12841 actggccagt cttgtcaggg ctggcgttcc gctcggcgtt atgctgcacc aggccctgcg
    12901 tgacatgggg aaaacctcat ggcattacgg tatcagcatt atccgggatc gtggaattga
    12961 cggtgcagcc ctcgacgtca ttgaagagcg gcatggtacc agcggtattg tctttgttga
    13021 cggatggaca ggtaaaggcg caattaccgg agagcttgta cgcgcactga aagatcgccc
    13081 gggctatcct gagcagccgc gactggttgt cctggccgat ccctgtggct gctcctggct
    13141 tgcagccagt gatgatgact ggctcattcc ttttggcatc atgggggcgc cggtatcagg
    13201 cctgatctcc cggtcagtat ggtcctctga aggattacac gggtgcatgg tttgcgagca
    13261 tctcagtgaa ttcgaatgca gccggatgct tgccgatacc gtcgctcatt tccgtaagaa
    13321 gttaacaccg tcatccctcg ctcccctgag ctggaatacg gaatcagccc gggtcttatg
    13381 gcagacaagt cgcgacgtta tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa
    13441 tcgtattaaa cccggtattg ctgaagcaac ccgggctgta ttacgtcggg taccggacca
    13501 tgtatttgtg cgttctattg acgacccgga cgttgccttg cttgtagggc ttgctcgtga
    13561 aaagggaata gttgttacag aaatgggggg aaccctcggc cagtatcggg ctgtaaccat
    13621 tatcaagaag gtactctgat gaaaaatcgt ctttctccct ggaatctggg agccacgcta
    13681 tacatgcctg caacacggga agatattgct gatgccgtcc tgcacgggaa aatcccgagc
    13741 ttgcgctctc tggtgatttg cctggaggat gctgtcagtg aagcagacat ccccgtcgcc
    13801 ctcaaaaatc tggagcacct gctgcacgag ctcagtaaca gcatgagtag cctgggtaaa
    13861 aatgactggc ctctggtgtt cattcgcccc cggcatgccg aaatgggcag atggttaaaa
    13921 gcgcattatg atctttccgc tgtcgacggg tttgtgctgc ctaaatttac cctcagttcg
    13981 ttagctgaat ggtgggatat catggtcggg actcacctgt gcatgatgcc aacgctggaa
    14041 acagaagatg tctttgacgt ggttcagatg cgcgagctgg ctacccgtct ggaggaacat
    14101 ccctgccacg accgcattat tgcgcttcga atcggcggca acgatcttat gaacgttgtg
    14161 tcgcttcgcc gtccccggga cctgacgctg tatgacagcc cgatgggcta cgtcatcaaa
    14221 atgctcgttt cggtcttcgg cccgcgtgat tttgccctga ccgcgcctgt atgtgagcat
    14281 attgacgatc atgccgttat ggccagagag ctggctcttg atatggcaca cgggcttgtt
    14341 gggaaaacgg ccattcaccc gggtcagata gaggtcattc aaaacgcgct gatggtcact
    14401 cagggtgagc attctgacgc tctgcggatc ctgaactcta cccaggccgt gtttaagtcg
    14461 cagggagcaa tgtgtgaacc cgccacacat cgccgctggg cggctggcat tctggacagg
    14521 gctcgttttt atgggttaca gaacgagcaa agcgctgatg gaatcagatt acttaccgtg
    14581 acccagcatc attaaggaaa tcataatggg gttcaggttc agaaagtcga tcaacattat
    14641 tcctggcgtt cgcctcaacc tgagtaacgg tgcaccgagc ctgagtgtcg ggccgagagg
    14701 tgcttccgtt tcttttggta gccgggggac ctatgccaat ctgggcttgc ccggtaccgg
    14761 gctgagttac cgtacccggc ttgaccgggc cgcgcgttcc agaggtgaaa accggacggc
    14821 aaccgacccg gggctcagac aggcgcttga gcagaaagcc gctgaactca tgtcagcggt
    14881 aaccgcaatc cgtaatattc acgagctgac gccggatcca aaaacaggca tcagctgggc
    14941 agagctggaa gcagtatacc tgcataacag aacgtcgcct tttcaggttc cggcaccggt
    15001 gcgtccagaa aagccagact accttgtatt gccggaaaag cctgccgaga gcgaaggcat
    15061 tagttttttg ggtaaatggt ttgaatcgga atcagctaaa gctgagcgcc acgccgaaaa
    15121 tcttcgccgg tggcagcagg agctgattga tgtggagcgt gagaataccc ttcgacagca
    15181 ccggtaccag caacaacgga cggcctgggc cgaacagtat gcaaactgga agtttgaagc
    15241 tgaagaacat gaaaaacggc tcgccacggc tcaggcagat gcccggcagc agttccggac
    15301 agacgccgcg tttttcgaat catacctggc gggtgtgctg gcagaaactg aatggccgcg
    15361 tgaaacgctt gttgcatttg aagtaaagcc ggagctatca gcagtcctgc tggacgttga
    15421 tttagctgag attgaagatt tccctgataa gatttacggc gttaatgccc ggggaacgga
    15481 gctgacggaa aaagccatgg cgcaaaaagc cgtacgcgaa aactatgccc accacgtcca
    15541 tggctgcttg ttccgcctgg tcggtatcgt tttacatacg ctacctttcg acaacgtgat
    15601 tgtgtcaggc tttacgcaac gggtcagtaa gcggaccggc tatctggagg atgagtatat
    15661 cctgtcctgc aaatgcactc gcagccagat gtcgtcagta aattttgcag gcataaaaca
    15721 cattgatccg gttgaagcgt taggcgatca accggttatt cgaaagatga gcagtacctt
    15781 catttttcag cctattgaac cactaaccct ttaagcaggc taaatcttac gatgcaatta
    15841 aataccagac aggcccggat ttttaagctt gccaacttac tgggcaccgg caagcccgtt
    15901 tccgctgccg acattataac cagtctggaa tgctctgagc cgacattaac ccgggccctc
    15961 aaagagctac gcgagtcata ttctgcggag atcaaataca gcaaggcagg tcattcctat
    16021 catctcgtta atccgggcca gttggataaa aagaccctcc gccggatgaa tgaagcgctc
    16081 gcacagaatg ctgaactgaa aaccggtgag tccacaggga aggtcgtact cgataaggat
    16141 aaaaaaacag ccgtgtcatt atcgctacgc atgcggattc taagaaaaat tgaccgtctg
    16201 gctgcgctga gtgactcgac ccgaagtgaa gcggtagaga agctggctct gcactccgtt
    16261 gatgaactga taaaagaata cagcgctaaa aagtcctgac gctaaatctc agaccggggt
    16321 gaaaactccg gttttgtcat atttcacctc tgtattggcg tgatgcaaaa tggcatctga
    16381 atcgatcatg gtttggcatc catttccgat cactgtttgg catcacaatc gatcacgatt
    16441 tggcatgctt ccgctcattg attggcatcc tggtgtcgtt acggggaagt catcttcaca
    16501 gggatcttca gtcagttagc attaccgaca tccattcacc aacttaccgg agataatcaa
    16561 tgtcaaaacc ccgatggacg cttgatcaga aaaaacatca cgttgccgca tggcgcgcca
    16621 gcggccttac ccgtgaacag tactgtgaac tttacgacat tccgtttaaa tccctgcgcc
    16681 agtggccgca ggatgtcgca aaagccgaaa aacgagccag agcaccagaa attattcctg
    16741 tttcagtatc tggctcttcc ggaatgactg atggcagacc attgtcggat gagcctgtca
    16801 ctctcttcct gcccggtggt atccggatgt gctgccagcc ttctcagtta accgatgttt
    16861 tccgggctct tcgccatgct gacgcctgat aacgtattcc tggtcgtaaa acccgtcgat
    16921 atgcgccggg gtatcgacac actgacacag tacgtccaga acgaacttaa cgctgcatgg
    16981 catgacggtg ctgcttttgt cttcaccaat aaagtccgct cccgcataaa agttctccgg
    17041 tgggacaaac atggcgtatg gctgtgtacc cgacgactgc accggggaag cttccgctgg
    17101 ccccggaaag gtgatgccac atggcatctc acacaggatg agtttcactg gctggtcttt
    17161 ggggttgact ggcaacaggt gaaagggcat gacctggcaa aatgggtata ccagtaagaa
    17221 cccgaagatg ctcagagcaa cgcaataact caatgaaata taatgaatat ccatgcgtga
    17281 tgtcatataa tccaccgcat ggatatctcg cttctcagta ccaccagtga ccctgaacag
    17341 cttcgcgctc tggcaattgc catggtgcaa aaagtcatgg ctgagaatgc agaacttcag
    17401 aaccgtatcc gcatcctgga agagcagatg aagctcgcac gccagcagcg tttcggtaaa
    17461 aaatgcgaat cgctggccgg gatgcagcgt tccctgttcg aagaggatgt tgacgcggat
    17521 atcgcagaaa tctcggcgca tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa
    17581 acaacaacac gtcctgttcg taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt
    17641 atcccgccag ctgaggaacg ttgtcctgac tgtgatgccc ccctgcactt cattcgcgat
    17701 gaagtcagcg agaagctgga atatattccg gcacaggtcg tggtcaaccg ctatatccgc
    17761 ccgcagtaca gctgtccctg ctgtgagaaa gtgttcagcg gaaaaatgcc ggcacatatc
    17821 cttccgaaaa gtgctgttga gccatcggtc atcgcacagg ttgtcatcag caaatatacc
    17881 gaccatctgc ccctgtatcg ccagcagcac attttctccc gaatgggggt ggagctcccc
    17941 gtcagtacaa tggcggatat ggtcggtgtt gctggcgctg cactggcccc gctggcaaaa
    18001 ttgttacgcc acgagcttct gacacgggat gttattcatg cagatgaaac gtccctgagg
    18061 ctccttgata cccggaaagg cggtaaatcc tgctccggct ggctgtgggc ttacgtcagc
    18121 ggagaaagag taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagtactac
    18181 gttccatggc aggagttcat caactcggtt ggaaggccat tctggcagta cgctcaggat
    18241 atggcgcaga tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta
    18301 caacagtgct ccacgctcgc cgccgtgatc gctgccaaag aacataaagt ttttctttcc
    18361 gagacagact gcacgaagcg ctctttccgc agcattatta tccgcctccg ccagaccgtc
    18421 atcactgtaa taacagaggg catcccactg attcagtaca tagctgcacg cttcgcccag
    18481 tctggatttt ttcgacagcg taccattctt ctccaccatc cattcatgca gtgacgtcag
    18541 taacactttg cttcgctgct gcctgacggc aagacgctct gactccggta atccccgtat
    18601 ttcatcctcg atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcact
    18661 tttgctgctg atgtatacat cgtggatttt tcgccgggca tgggcccagc acgcaacttc
    18721 tgtcagtgca ccaccttcac gttctgcact gaacagccga tcgtaaccgc tgaacgcatc
    18781 cgcctgcagg ataccccgga agggacgaag gtgttgtacc ggatgttttc cctgcctgtc
    18841 tggtgagtag gcgaaccaga ccgccggtgg ctctgatgag cccgcattcc ggtcatcccg
    18901 gacatacgtc cagatgcgtc ctgtttttgc cttttttctg cccggtgcca gcacttttac
    18961 tggtgtgtcg tcagtgtgaa ccttgcgggt gttcatcacg taacggtaca gggtatcatt
    19021 cagcagagtc attaactgac aacatgcgtc aacccagttg gagagtaatg cccggctcag
    19081 ttcgatgccc tggcgggcaa agatttcact ctgacgatac agtggcaggt gttcgcagta
    19141 ttttcccgtt aacacgcggg caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg
    19201 ggacggcgct ggcgcttcaa ctatacagtc acattttgta caggcttttt ttacccgaac
    19261 agtgcggatc actttcaggg cgctactcac cagttccagc tgctcagcac tgacttcccc
    19321 cagataatcc agctcactgc cacactccgg gcaacaactt tcttcaggct ccagacggtg
    19381 tatttcacgg ggaagatgtg ccggtaacgg acgacgatga cgtgactgtc gcaactggcg
    19441 gggaacctgc gggtcatcct cacgcccact gtaacgatcg ctttcctgtt cgcgttgttt
    19501 cagttgggcc tcagcctgtt caacctcacg ctgcagtttt tcagaacggg taccgaacaa
    19561 catccggcgc agtttttcta tctgggccct cagatgttct atttcccgct cctcctcttc
    19621 gatcttttcc tcggcacgtg ccagtgcaga gcgcaggaag gcctccgtct cttcaaccag
    19681 actcagttgc tgatctttct gacggagggc ttcagcctgc tcagagagca gcctttccag
    19741 ctcagtgata cgaatgaggt atttccgact catgaccgtt tttataatcc ggccatgaca
    19801 tttttacaac attgtcagtg cattaaggcg ggatgttttg ggctgacgcc agtccagttt
    19861 atcgaggagc attgccagct gcgagcgggt aatggatacc ttaccgtcac gcaccgcagg
    19921 ccagataaac tggccttcct ccagacgttt ggtgaacagg cacagaccat cagcatcagc
    19981 ccacagaatt ttaatcgtgt caccccgtcg gccgcgaaag ataaacaggt gaccggagaa
    20041 ggggttctca tccagcacat gttgtacctg ttctcccaga ccgttgaagg atttacgcat
    20101 atcagtaacg ccggcaacca gccagattcg agtgcctgat gggagcgaga tcatcgtccc
    20161 ctcccggtca gttcacggat caacaccgtg agcagctctg gtgaaggatt ttccagcgtc
    20221 atgttaccgt ggcggaactc aactttacag gaactggcac tgactgtgct ttgtgaagga
    20281 gtggataaaa gcggagtaag agccgccata ggctctttct gctcatcagg cgttatctca
    20341 acaggtaata attcaacgcc agcgccagaa gaggttgtta ccggaagacg ccgcgatata
    20401 cgcctttcgt tctgccagag cctgagccat ttgaacagga ggttatcatt gatatcgtgt
    20461 tcccgggcaa tacgggcaac agaggctcct ggttgtgaag ccagtttaac catttgaagt
    20521 ttaaactcat ttgaaaatga tctgcggggt tctgaggata atattttctg ttccataaca
    20581 ggtgtccact agttgaaaag gtgggcacct acgttaccaa tactggctta atggctacat
    20641 acggcggtca gtttacgctt acgagaaaga agcggtccgc cggtcgtctg ttttgactcg
    20701 cagaccggac gggccctcag atatcctgag gcctggctgc agggctggtg tggcggtacg
    20761 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatgaccc cggtattaca
    20821 tctgcctgtt gctggagcca cgcaaggcgc ggtttcgcaa atctgtataa ggc
//
LOCUS       DAZGXS010000077        20345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGXS010000077 DAZGXS010000000
VERSION     DAZGXS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(205..951)
                     /gene="cutC"
                     /locus_tag="ACHKG5_002991"
     CDS             complement(205..951)
                     /gene="cutC"
                     /locus_tag="ACHKG5_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0869052.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(965..1531)
                     /gene="yecM"
                     /locus_tag="ACHKG5_002992"
     CDS             complement(965..1531)
                     /gene="yecM"
                     /locus_tag="ACHKG5_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0869053.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1747..3480
                     /gene="argS"
                     /locus_tag="ACHKG5_002993"
     CDS             1747..3480
                     /gene="argS"
                     /locus_tag="ACHKG5_002993"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0869054.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3522..3566)
                     /locus_tag="ACHKG5_002994"
     CDS             complement(3522..3566)
                     /locus_tag="ACHKG5_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869055.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3657..4145
                     /gene="yecT"
                     /locus_tag="ACHKG5_002995"
     CDS             3657..4145
                     /gene="yecT"
                     /locus_tag="ACHKG5_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP0869056.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4265..4657)
                     /gene="flhe"
                     /locus_tag="ACHKG5_002996"
     CDS             complement(4265..4657)
                     /gene="flhe"
                     /locus_tag="ACHKG5_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0869057.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4657..6735)
                     /gene="flhA"
                     /locus_tag="ACHKG5_002997"
     CDS             complement(4657..6735)
                     /gene="flhA"
                     /locus_tag="ACHKG5_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0869058.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6728..7876)
                     /gene="flhB"
                     /locus_tag="ACHKG5_002998"
     CDS             complement(6728..7876)
                     /gene="flhB"
                     /locus_tag="ACHKG5_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0869059.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8078..8722)
                     /gene="cheZ"
                     /locus_tag="ACHKG5_002999"
     CDS             complement(8078..8722)
                     /gene="cheZ"
                     /locus_tag="ACHKG5_002999"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0869060.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8733..9122)
                     /gene="cheY"
                     /locus_tag="ACHKG5_003000"
     CDS             complement(8733..9122)
                     /gene="cheY"
                     /locus_tag="ACHKG5_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0869061.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9137..10186)
                     /gene="cheB"
                     /locus_tag="ACHKG5_003001"
     CDS             complement(9137..10186)
                     /gene="cheB"
                     /locus_tag="ACHKG5_003001"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0869062.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10189..11049)
                     /gene="cheR"
                     /locus_tag="ACHKG5_003002"
     CDS             complement(10189..11049)
                     /gene="cheR"
                     /locus_tag="ACHKG5_003002"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0869063.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11068..12669)
                     /gene="tap"
                     /locus_tag="ACHKG5_003003"
     CDS             complement(11068..12669)
                     /gene="tap"
                     /locus_tag="ACHKG5_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0869064.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12715..14376)
                     /gene="tar"
                     /locus_tag="ACHKG5_003004"
     CDS             complement(12715..14376)
                     /gene="tar"
                     /locus_tag="ACHKG5_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0869065.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14519..15022)
                     /gene="cheW"
                     /locus_tag="ACHKG5_003005"
     CDS             complement(14519..15022)
                     /gene="cheW"
                     /locus_tag="ACHKG5_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0869066.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15043..17007)
                     /gene="cheA"
                     /locus_tag="ACHKG5_003006"
     CDS             complement(15043..17007)
                     /gene="cheA"
                     /locus_tag="ACHKG5_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP0869067.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17012..17938)
                     /gene="motB"
                     /locus_tag="ACHKG5_003007"
     CDS             complement(17012..17938)
                     /gene="motB"
                     /locus_tag="ACHKG5_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0869068.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRVLRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17935..18822)
                     /gene="motA"
                     /locus_tag="ACHKG5_003008"
     CDS             complement(17935..18822)
                     /gene="motA"
                     /locus_tag="ACHKG5_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0869069.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18949..19527)
                     /gene="flhC"
                     /locus_tag="ACHKG5_003009"
     CDS             complement(18949..19527)
                     /gene="flhC"
                     /locus_tag="ACHKG5_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0869070.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19530..19880)
                     /gene="flhD"
                     /locus_tag="ACHKG5_003010"
     CDS             complement(19530..19880)
                     /gene="flhD"
                     /locus_tag="ACHKG5_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0869071.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4902 a         5577 c         4936 g         4930 t
ORIGIN      
        1 agttttagca atttaaatca cataattaat gtgaatatat aaaatatttt tagcacaacg
       61 ctatgtgcac tgtcacccgc tcaacagatc gcggtaatcg ttcatttttt atactattca
      121 gggataaaaa aaggcccctg ttgaaattgc aggggcctgg tacgagcaag catcatattg
      181 ggcgacatga tgcaacggta aaaatcattt ggcctgatgg cgttcaatga ttcctttcat
      241 ttcagcaacc gccgccccgt ctacgacata acgcgaatac tcgtccgcgt gtgcatctga
      301 tgacatggac aatccttgat tacgataacg catcggtgag gcttgccacg ctcccgcgga
      361 gctatggact tccagcactc cggcatcgag gaagtggtgc aggttttctg cacggactcc
      421 tgctccggcc ataatgattg gagcatcacg atgggcaata agttccataa tttttgataa
      481 accttgcagt gcgtctgatt tttgccctga tgtcagtacc cgggcaatgc ccaattccgc
      541 aagattattg agtgtattta gagggttagc gcacatatca aaggcgcgat gaaaagtcac
      601 tgccagcgga ccggcagcag ccattatttt ttccattcgt gacatatcga cattaccgtc
      661 aacatcgagc acgcccgtca cgagtccagg aaaacctaat tcgcggaccg tgcgcacatc
      721 ctcaagaatg gcggcaaact caccgtcgct gtagcaaaaa tcaccaccgc gtgggcgaat
      781 tatcggatgc acagggatcg tcacccgctg gcgcacggat ttcagtacac ccaacgacgg
      841 cgttaagccc ccctcttttg gggctgcgca taattcaact ctgtctgcgc cgttttgctg
      901 cgccgttagc gcgcattcca tgctgtaaca gcaaatttcc agtaatgcca tttttgctcc
      961 ttaattacgc cgactgctcg ctggcaacaa tctcttcaat ggaccacgga tgaaatttaa
     1021 tggtcgtttt gccatcggtg accgccagcg tcggattcgg taatcgttca tgctcacctt
     1081 ttggcgaact ggttttaact gagatccccg gtaggctgag tccttcatca gaaagcaatg
     1141 ccagcgcacg ggcattcagc gtttctggat cacccggcaa aacgatttca atatgttccc
     1201 aaccttcgtg cgggtaacgt ttttccctag gccacggtag ctccacaata gaaaactgcc
     1261 agtgtgcaac ctgtaccggt tcatgcaatt taaacagaca aatcggtctg ccattgatca
     1321 tattttctga caaaagctcg ccacactgtt caaacccgcg acgccagcgc tcagcagtgg
     1381 cgttttgatg gcaacgcaaa gaaatgtgat cggcagtcag cggagtgata ttcaacccca
     1441 gacggcggga aagttcatct aatgcgtggg taaatcgcgg taaatccgat gcaatatcct
     1501 gcagctcgtc gatagattgc cagttcgcca taatcactct tcgtctttca gtaaaagcgt
     1561 taatttaccc tgttgccctg cgccaaccaa ccgctgattt cacgccgctt ctgatgcaat
     1621 agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa aacagccatt tgcagtatac
     1681 tcccgcccta atttctttaa ctggtgcggg caatttttgc tcgcttcatc aatgtaaggt
     1741 attccggtga atattcaggc tcttctctca gaaaaagtcc gtcaggccat gattgcggca
     1801 ggcgcgcctg cggattgcga accgcaggtt cgtcagtcag caaaagttca gttcggcgac
     1861 tatcaggcta acggcatgat ggcagttgct aaaaaactgg gtatggcacc gcgacaatta
     1921 gcagagcagg tgctgactca tctggatctt aacggtatcg ccagcaaagt tgagatcgcc
     1981 ggtccaggct ttatcaacat tttccttgat ccggcattcc tggctgaaca tgttcagcag
     2041 gcgctggcgt ccgatcgtct cggtgttgct acgccagaaa aacagaccat tgtggttgac
     2101 tactctgcgc caaacgtggc gaaagagatg cacgtcggtc acctgcgctc taccattatt
     2161 ggtgacgcag cagtgcgtac tctggagttc ctcggtcaca aagtgattcg cgcaaaccac
     2221 gtcggcgact ggggcactca gttcggtatg ctgattgcat ggctggaaaa gcaacagcag
     2281 gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag
     2341 cattacgacg aagatgaaga gtttgccgag cgtgcacgta actacgtggt aaaactgcaa
     2401 agcggtgacg aatatttccg cgagatgtgg cgcaaactgg tcgacatcac catgacgcag
     2461 aaccagatca cttacgatcg tctcaacgtg acgctgaccc atgatgacgt gatgggcgaa
     2521 agcctctaca acccaatgct gccaggaatt gtggcggatc tcaaagccaa aggtctggca
     2581 gtagaaagcg aaggggcgac cgtcgtattc cttgatgagt ttaaaaacaa ggaaggcgaa
     2641 ccgatgggcg tgatcattca gaagaaagat ggcggctatc tctacaccac cactgatatc
     2701 gcctgtgcga aatatcgtta tgaaacactg catgccgatc gcgtgctgta ttacatcgac
     2761 tcccgtcagc atcaacacct gatgcaggca tgggctatcg tccgtaaagc aggctatgta
     2821 ccggaatccg taccgctgga acaccacatg ttcggcatga tgctgggtaa agacggcaaa
     2881 ccgttcaaaa cccgcgcggg tggtacagtg aaactggccg atctgctgga tgaagccctg
     2941 gaacgtgcac gccgtctggt ggcagaaaag aacccggata tgccagccga cgagctggaa
     3001 aaactggcta acgcggttgg tattggtgcg gtgaaatatg cggatctctc caaaaaccgc
     3061 accacggact acatcttcga ctgggacaac atgctggcgt ttgagggtaa taccgcgcca
     3121 tacatgcagt atgcatacac gcgtgtattg tccgtgttcc gtaaagcaga aattgacgaa
     3181 gagcaactgg ctgcagctcc ggttatcatc cgtgaagatc gtgaagcgca actggcagct
     3241 cgcctgctgc agtttgaaga aaccctcacc gtggttgccc gtgaaggcac gccgcatgta
     3301 atgtgtgctt acctgtacga tctggccggt ctgttctctg gcttctacga gcactgcccg
     3361 atcctcagcg cagaaaacga agaagtgcgt aacagccgtc tgaaactggc acaactgaca
     3421 gcgaagacgc tgaagctggg tctggatacg ctgggtattg agactgtaga gcgtatgtaa
     3481 tcgatttttc gtgagagtga agcctgatca ggtttagccg atcaggcttt tttattacca
     3541 tctaaatgta ttctgaaaat aggcatgcca ttgttttctc gctgttgagt aagaggccag
     3601 aagcgtaata tccagctcca gggaaacggt aacggttcaa tttaaggaat accgcagtgt
     3661 ttaaatttct tgtattaaca ttaggcatta tctcttgcca ggtttacgca gaagatacgc
     3721 ttatagtaaa tgatcatgac atttcagcca tcaaagattg ttggcaaaaa aattcaggtg
     3781 atgatactga cattaacgtg atcaaatcat gtctgcgaca agaatacaat ctcgtcgatg
     3841 cgcaattaaa taaagcttat ggtgaagctt atcgttatat agaacaagtg ccacgcacag
     3901 gtgcaaaaaa acctgatact gaacaactta acttgcttaa aaaatcacaa cgagcctggc
     3961 tggattttag ggacaaagaa tgtgaattaa tcctttcaaa tgaggacgtt caggatttaa
     4021 gtaaccctta ttctgaatca gaatggctat catgtatgat catacagacc aatacgcgaa
     4081 ctcgccagtt gcagctatac cgtaactctg aagattttta tccaagccct ttgacaagag
     4141 gataattcac atatttttgg catgttttgt tgcaagctat tcctgataaa tgattgcaac
     4201 aaaacatcca gcctttttca ctgagttatt aaacatactc gcgagcgcgt aatttttttg
     4261 tccttcagcg ataattcaca atcacttcat tacgttgtac tttcagcgac ggaattaacc
     4321 gcccaccgcc aggcacttcc cagataaagc gcaacggttc tgctgccgct ataccggaaa
     4381 aggccacggt ggttccgctc tgcccatcta attcgacaca acgagattgc gaacacaagc
     4441 gaacccgcag tcctgcaggt gtcgggccga taagctgata ccgccacgcc accagcgtca
     4501 tcaatcctga agcaggttgt cgcgtagaaa gaggtgcaga cgacatcgac tcaccgcgat
     4561 gatttagcgt aattccgaca ctacttgcct gccacatccc ctccccggcg gcttgcacca
     4621 gcagcggaaa caataatatt gctaataagg ttctcatcat ttaccgccaa ttgtcgccgt
     4681 catgcggata tgtcggttat cagacagttc cagattcgac agcaccacta actgcggcaa
     4741 gctgcggcgc agaaagcgag acaataatgg tcgcagcgcg tggttcacca ataaaactgg
     4801 cggcgcaccc aacatctcct gacgggatag cgcttcctga gtttgcgcca gtaaacgatc
     4861 cgccagccct ggctccagtc cgcccccgcc ctgcaacgcc tgtagcaaca aacgttccag
     4921 cggtgtatcg aggccaataa catggacttc atctttgcca ggaaaccact gctgggtaat
     4981 cgcccgtccc aacgccacgc gcacgacggc ggttaattca tgtggatcgc tttggatggg
     5041 cgcatgttcc gccagcgttt cgagaatagt gcgcatatcg cgaatcggta ctttttcatc
     5101 gaggagattt tgcagcactt tatgcagtgt ggtgagcgtg acgacgccag ggacgagatc
     5161 ttccgtcagc tttggcatct cctgggcgac gcgatccagc agctgttgcg cctcctgacg
     5221 accaaacagc tctgcggcat gctggctaat gaggtggtta agatgcgttg ctaccaccgt
     5281 gctggcctca accactgtgt acccctgaat ctgcgcctgt tcttttagcg cactttcaat
     5341 ccagatagca ttcaggccaa atgccggatc gacggtcgcc tcaccaggta acgtcccggc
     5401 agcggttcca gggttaatag ccagccagcg ccccggataa gcatcaccac tgcctatctc
     5461 cacgcctttc atcaaaatgc gatagcgggc aggttgcaga tccatattgt cgcgaatgtg
     5521 caccactggc ggcagaaatc ccatctcctg ggcaaatttc ttgcggatac tgcgtatacg
     5581 gcccaacaac tcaccatcct gctggaaatc gaccatcggg atcagtcgat aacccacttc
     5641 cattcccaga gaatcttcca gttgtacatc gttccacgtc gcttcgacaa cggcattatt
     5701 ctctgccatt tttaccggtt ttggttcggc aggcgctttt tgttcgcgtc cacggatcca
     5761 ccaggccagc cccagcaatc cggcagtgaa cagcaaaaat accaggttcg gcattccagg
     5821 caccaggccg agtaaaccga gcacggcagc gcttaacaac ataacgcttg ggttactgaa
     5881 aagctgattc accatctgct cgccaacatc ctgatcggtg ctgacacgcg taacaatgac
     5941 ccccgcggcg gtagaaatca ccagagccgg gatttgtgcc accagaccgt caccaatggt
     6001 caagagcgta taactttccg ccgcgtgtcc catgctcatg ccatgttgca gcacgccgac
     6061 cagcaacccg ccgacaacgt taatgaccat gatgaggatc ccggcgatgg catcgccgcg
     6121 aacaaactta cttgccccgt ccattgagcc gtaaaaatcg gcttcctgag tgacttcgga
     6181 gcggcgtttt ttcgcctcat cttcaccaat caatccggcg ttaaggtcgg cgtcaatcgc
     6241 catctgctta cccggcatac catcgagaac aaagcgcgca cccacttctg cgatacgccc
     6301 ggcacctttg gtaatgacca taaagttgat gatcacgaga atgacaaaca ccacgatacc
     6361 gatagcgaaa ttgccaccaa cgaggaagtg accgaacgct tcaaccacct tccctgccgc
     6421 cgctgcgccg gtatgccctt ccattaaaat gatacgggtt gaagccacgt taagtgccag
     6481 acgcaacagc gtggtaaaca acaaaatggt cggaaacgca gcaaactcaa gcgtgcgctg
     6541 ggtaaacatc gccaccagca acaccatgat cgacaaggca atattgaagg taaacaacag
     6601 gtcgagtatg aatgcgggca gtggcagcac catcatcgac aagatcaaca ggatcaaaat
     6661 cggtccggca aggatctgcc attgtgtcga tttcaggttt gtgggcaggc gcagcatcgc
     6721 ggccagatta ctcatgggtc ggtttctcgt taataaaatc cagggcttcc ggcaccggaa
     6781 gatgagtagg ttgtacaggg cgctgtccac cagccagacg ccagcgtttc agttgccaga
     6841 cccaggccag cacttccgcc accgcggcgt acagttgacc cgggatttgt tgaccaatct
     6901 ccacatgtcg atacagcgct cgcgccagcg gcggcgcttc aagcgtcggg acgttatttt
     6961 cagcgccaat ttcacgaatg cgcagcgcga ccagccctgc acctttagcg accactttcg
     7021 gtgcgctcat tttgttttcg tcatactgca acgctaccga atagtgggtc gggttattga
     7081 caatgacatc cgctttcggc acatcggcca tcatccgacg ccgtgcggca gctcgctgca
     7141 tctgacggat ccgcccttta acatgggggt cgccttcgct ttgtttgaac tcatcacgaa
     7201 tatcctgccg cgacattcgc agctttttca ggtggctgaa gatttggaaa aagacgtcaa
     7261 atcccaccat tggaatgaca ccaagcacca ccagcagtgc gcatagccct accaaatcca
     7321 tcgcattacc catggcggta atcggagact cggccattaa gcgcatcata tgcggccagt
     7381 gatgccagag aaaaaacccc gtcacgctgc caaccaggat ggttttcaaa attgctttaa
     7441 gcaactccgc gccagtctgt gccgagaaca tccgttttat gcccggtagc gggttgagtt
     7501 tggaaaactt cggctgcaag gattttccgc taaataccag cccccccagc ataaccggag
     7561 aaatgagcgc caccagcacc acgccgctaa tcagcggtag cagcgccagc atggcttctc
     7621 tgatcagcag aataatttgc ccgaggatca gattcgggtc tttgataata ctgtgatcaa
     7681 aatgcagccc agcggagagc atgcccgaca atcgacgggc cagcgacaca ccgccaaacc
     7741 agataacact aacgcccacc agcaaaatca gcagtgaggt cagttcacgg gaacgcggga
     7801 tttgcccctc ttcccgcgct ttttctagtc ggtgaggtgt gggggcttct gttttgtcgt
     7861 cgctctcgtc agacacgtcg ccaatcctgg aaagagtatt aagcgtaaat gatgccagag
     7921 cgcaaagcgt tcaatggttt gagtaagggg caaaacaggc gggatttagg gcttttgctg
     7981 ccacacatca agcatagtgt gcgtttgtcg gatgcggcgt catcgcctta tcagaccgcc
     8041 tgatatgacg tggtcacgcc acatcaggca atacaaatca aaatccaaga ctatccaaca
     8101 aatcgtccac ctgatcctga ctggctacca caccggcttt gctggtatcg acctgaggtc
     8161 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
     8221 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaatga
     8281 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
     8341 taaagctggt atgcgcgggt acatctgcca gaaatttccg cgtatctgtt accagttcac
     8401 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
     8461 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
     8521 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
     8581 tggcttccgc cgcttcggca atggcctgat ccagtcccag ttcccgcaaa ctgtcgcgca
     8641 gcatacgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     8701 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     8761 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     8821 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     8881 aatgccgaca tcgcgccatc cgcacgaatc gttttcagca attccaggcc atccatgttg
     8941 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     9001 tcgacgccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     9061 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     9121 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattttt gccaacattt
     9181 gctggcttac ctggttaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     9241 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gccccagcct
     9301 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     9361 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     9421 gacggttaac cgccgggcca tcgtgaattt tgatttggta atttgcgcca ctacgcgcca
     9481 gctccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaaaaca cgttccccgt
     9541 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     9601 tgaaaccggg cggcatatgc tgggtaatta acagtgccgg gctggaaagc ggcaacggtt
     9661 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     9721 cagaactcaa caacggccct gccttcagcg ttgtcggtgc cgacaatggc ttatgtgctg
     9781 caaggctcgc ctttgctgct gtacgcacct tttcagcaat catttcgctg tacgccagca
     9841 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     9901 cgcgcagcgt gacttctgat cctttgccgg tcagggaaga aaccatcaca acgggcattg
     9961 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
    10021 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcgacc agcggatcag
    10081 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
    10141 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
    10201 tagcgcatac accgtttgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
    10261 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
    10321 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
    10381 cggccccggc acggtgtact gtttcgccag tagattcagc gaggcaaaat caacatagtt
    10441 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
    10501 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
    10561 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcgc
    10621 ggtgcccaat gtgtcagcca gcgtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
    10681 cgccgcgctc catacgcgat actcgccaga acggcgacgt gcgtgatccg cgagcagagg
    10741 gaaatgatgt gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
    10801 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
    10861 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
    10921 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
    10981 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
    11041 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
    11101 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
    11161 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgaggc
    11221 gttctgctgt gtcacctgat ccatctggct gacagcctgc gcaacctgct caatcccccg
    11281 ccgttgttct tccgaggccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
    11341 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
    11401 acgattgact gactcttcga tcagcccttt gatctctttt gccgcctggg cgcttcggct
    11461 ggcaagattg cgcacttctc ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
    11521 agccgcttct actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
    11581 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
    11641 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgcaagttc
    11701 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
    11761 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
    11821 gcctgcgaca atctccgcga taccaatgtg catctcctgg cttcccttac gcacatcact
    11881 taccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
    11941 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
    12001 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
    12061 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
    12121 acgctggctt tgcgcactgg cggcttccag cacatggatg atctccagct gccaggcctc
    12181 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
    12241 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
    12301 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
    12361 acttttaaac agcgtggtgg attgcgtcag actggcacgc gccgtcgtca tcaacgtttt
    12421 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgtggtact
    12481 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
    12541 gacctgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
    12601 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
    12661 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaata ctcgtcaaaa
    12721 tgtttcccag tttggatctt gttcagcaat tcgcagtcgt ggctgtgccg gtggttgctc
    12781 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacgaaa
    12841 cgcggaaacc gcttgcgtta aacgactagc ctgttcttcc agcgcagctg cagcggcagc
    12901 tgattcctgc accagcgatg cgttctgttg cgtgacgtga tccatttccg aaaccgccaa
    12961 tgcgacttga tcaatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
    13021 gtcagtcacg cgagtgacag catcgacgat attgttcatt gtttccccgg cgctttcgac
    13081 cagcaccgaa ccggtatcaa cgcgcgagac ggagtcttca atgagagctt tgatctcttt
    13141 tgccgcctgg gcgctacgac tggcaagatt acgcacttcg cccgccacca cggcaaaacc
    13201 acggccctgt tcacccgctc gcgcggcttc aacagcggca ttcagcgcga ggatattagt
    13261 ctggaaggca ataccgtcga ttacgctgat aatgtcggcg attttcttcg aactatcggc
    13321 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
    13381 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
    13441 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttccgt
    13501 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
    13561 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
    13621 ctgcgccagg tcgcccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
    13681 accggcgatt tcgcgaatgt gagcaataat ttttgccagc ggtgtaagca acatacggcg
    13741 aatgccatac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
    13801 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
    13861 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
    13921 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
    13981 tgtgtaatag tttttatatt tttcatcaat attacgactg gtagcgacca tttcaggtaa
    14041 cggtgccatg cttttgaatt ttttataatg agtcgcggcc tgggccaatg ttttcctggc
    14101 gctatcgagc aattcaactt tggcattact ttgttgattg gaggaatcca tcatcattcg
    14161 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
    14221 ctcgccctgc tgttctcgta attgattgga aaccacaaag ctcttctggc tatggtgaag
    14281 ggaagaaaaa aacagactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
    14341 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
    14401 gatctcgtta tcggcaagga agggggaaac tttattgctg atgccacccg ccgcgaaatt
    14461 gaaataaaac ccgatgcgca gatcatcggg ttcatttcaa ttgaggaaat caggagaatt
    14521 acgccacttc tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc agcagttttt
    14581 cgatgttcac cagaatcaac atacggtcgc ccagtgcgcc cagtccagtg agatattctg
    14641 ttgaaagcgt cacggcaaac tctggtgccg gacgaatttg ctccgccgtc aatgaaagca
    14701 cgtctgaaac gccgtcaacc acgatgccga ccacccgctg tccgagattc aggacgataa
    14761 ctaccgtgtt gtcgttatag tccacatcca cctggctgaa cttaattcgt aagtcaacaa
    14821 tcggcacaat aacgccgcgc agattcgtga cgcctttgat aaacgctggc gtgttcgcaa
    14881 tccgtgttac ctgatcgtag ccacggatct cctgcacttt caggatatca ataccgtact
    14941 cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg gccagctttg
    15001 ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg cggtgttcgc
    15061 catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct
    15121 gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt tactttcaag
    15181 gtttttaact acaacctgat gttgaccaat taattgatcc accagcaagg cgtagcggcg
    15241 accgccactt tgtaagatca ccacaattcc ctgggtagct tcggttttcg cgcccgcgac
    15301 gttgaacact ttccacagtt cgacgatggg cagatattca ccccgcactt ccagcacccg
    15361 ctcgccgccg gccagtggat gcagatcggc ttcacggggt tgcagtgatt ccataacagc
    15421 attcagcggc agaatgaata cttcatccgc aacgcgtacg gacatgccgt cgaggatggc
    15481 cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta ccctgcttcg actggatttc
    15541 aacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga cgccgcgccc
    15601 ggagacgtcg gtgacctgct ctgccgtgga gaagcctggt gcaaatatca gcatcgcgac
    15661 ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc gaggccgctt ttgccagaat
    15721 tcgctcacgg tttagccccg ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg
    15781 atgttcggca gacagaatta aatttccgac gctgttttta cctgcggcga gccgtttttc
    15841 tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat
    15901 gcgttctatc aggctcttgt cgagctcggt ggaactgccc accagcgtca gttctacctg
    15961 cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa agacatattc
    16021 catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa
    16081 ctgccccatg ctggtaatca aatcaccatg attaaccggg tccagttcgc tggaacgctg
    16141 ggcaagcatg gactgggtga taaccagctc gccgacgagg ttaattaatt gatcaacctt
    16201 ttctaccgct acacggatgc tggtggattc actgctacgc gtcgtttttt cccgctccac
    16261 gcgaccggtt ggcgcttgtt cggctgccag tttaagcact ggtggggtgg atatttttgg
    16321 cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac agagtaccgc
    16381 tgtgatgtca tcttcggcga tatcgcccgg taatattgcc gagagagaat ccgccccttt
    16441 caccacgtca gttaacgttg tcagatgccc cagctcttct tccagcaggt cgacttcgct
    16501 ggccttcagg cgcgaaagga taattcgtcg cggcaactga ctgcgactct gctcatcttg
    16561 cggttcactt ttggcaacca cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc
    16621 ttctaatgcc agttgacgca aggcctggca gatataatcg aagctggcgg catccggctc
    16681 ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag
    16741 attgataatg tcggtgttga gttgcatctc acctcgtctg gcttcatcga gcaggttttc
    16801 catcagatgc gtggtttcct gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat
    16861 cgagtgggca gcccgaaaga tggcattcaa ttgttcggca tctggcgctt ccggctgcaa
    16921 aaccagcaga tgctgctcca tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg
    16981 ataaaaatcg cttatatcca tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg
    17041 gaacactgac ctgtggtgca acctcaggtt tttccagggc gcttactggc tcattctggc
    17101 tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc agcaggctga
    17161 tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc
    17221 cgaccacacg taacactttg ccgctatcca accctccgac catcagttcg cggcgggatg
    17281 cattggcccg atcggcagaa agctcccagt tgctgtaacc tttctcacca ctggcgtagg
    17341 ggaaatcatc ggtatgaccc gaaaggctaa tacggttggg aataccattc agaacgggcg
    17401 cgatggcacg cagaatgtcg cgcatatagg gttcgacgtc ggcactgcca gttctaaaca
    17461 tcgggcgatt ctggctatcg atgatctgaa tacgtagacc ttcctggacc agatcgattt
    17521 tgagatgtgg acgtaacacc cgcagtttcg gatcggactc tatcaattgg tcgagatcgc
    17581 cacgcaattt ccgcaatcga ctttgctcca tgcgtttttt caactcttcg atgttcggct
    17641 gcttattcac ttccccctgg ctttgggtgt aatcatcgcc accgccggga attgggcttt
    17701 cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc cagtggagtc cggaagtact
    17761 ccgcaatctg aatcaactct tttgggctgg agatggagat cagccacatc accagaaaaa
    17821 aggccatcat cgcggtcata aagtcggcat aagcaatctt ccacgatcca tgtgctgccc
    17881 cgtggctttt ggctttgcgt cgtttgacga caataatcgg atgcgcttga ttcttcatgc
    17941 ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct cttccagttc
    18001 aataaacgac ggacgttcgc tggaatagag cgttttgcga ccaaactcaa cggcgatagg
    18061 cggtgcgtaa ccgttcagat tagaaagcag tgtgactttg acgcactgca tcattttgct
    18121 ggtttcggcg cttttctgac gtaaaacact cgctaatggg gaaataaatc cgtaagccaa
    18181 taaaatgccg aggaaagtcc ccaccatcgc atgtgcgata agcgccccca gctcggcggc
    18241 aggacgatcg gctgaaccta acgcgtgaac gacccccatt acagccgcga caataccaaa
    18301 cgccggaagt gagtccccga ccagcgccag actgttcgcc gggacttctg cctcgctttc
    18361 gtgcgtctca atctcttcat ccatcagggc ttcgatttcg aaggtgttca tgtgaccgct
    18421 gataatcagg cgcagataat cgacgataaa atcaagcatg acgctatccg cgatgatgcg
    18481 tgggtagctg gcgaagatct cgctctcacg gggattttca atatcacgtt ccagcgaaaa
    18541 catccccatc tgccgcgatt tcgccatcaa ccgataaagc agagccagca gatccatata
    18601 cattgctttg gtgtatttgg agcgacgaaa cagcaacggc agcgctttca gcgtgccttt
    18661 aatcgctttt ccattattgc cgacaataaa cgacccaatc cctgcaccgg caataatcac
    18721 cagttcagcg ggttgataga gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa
    18781 aactgtaccg agaacaacca ggtaacctaa taagataagc acgacatcat ccttccactg
    18841 ttgaccatga caggatgttc agtcgtcagg cgttaacgcg cgattggggc aaaaaaaagc
    18901 agcggtacgt cgttaccgct gctggaatgt tgcgccacac cgtatcagtt aaacagcctg
    18961 tactctctgt tcatccagca gttgtgggat aatatcggca ggattctggg aaagtttacg
    19021 tctttttact gcccgggatg gcggttgaca taagctgcag gcaaagctgc caacaggctg
    19081 gtgagcgtgg gtaataaaat tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc
    19141 actttcaaca aaccgcacca atgtccaggc acgggttaat gccagcagcg gtccttcttc
    19201 tgcttgcggg cactgttcaa ggtataaacg gtaggctttg atcaccgcat cgacgccatt
    19261 acacaaaccg gttttcagta aaaactgcca tgcattacag aacatcgaag catgaacgtt
    19321 ttgttcccaa gtcataaacc agtcggttga gaatggcagc atgcctttcg gcggtgggct
    19381 tccgcgcagt tctttataaa gttttatcag gcgtccgcga cttaactgtg tttcgctttc
    19441 cagcatctgc aaacgagcgc ccagggtgat caattccatt gccagctgaa tatcccgcgc
    19501 ttcctgaaca atgctttttt cactcatgat caggcccttt tcttgcgcag cgcttcttca
    19561 ggctgattaa catcattcag caagcgtgtt gagagcatga tgccggtatg aatttgctgg
    19621 agatcgtcaa cgcgggaatc ttgcgtcaac agagtaatcg tctggtggct gtcaaaacgg
    19681 aagtgacaaa ccagctgatt ggtttctgcc agcttaacca tttgcggaag agtcagtgcc
    19741 gctaatgttg tcgccatttc ttcatttatg ccgagacgaa acatagcgga cgctttgtcc
    19801 tgaacaatca gacgctgtgc aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc
    19861 agcaactcgg aggtatgcat tattcccacc cagaataacc aactttattt ttatgcggtt
    19921 tcaccgcacc ccgtgatgtc gccgggaagc cccggtaaaa aataattagc attagaatag
    19981 ttgcgataag ctgcaataag cagagccacc tttttggttt aataagtcct tacaaataga
    20041 agggggtctt tacacttatc taagattttt cctaaatcga cgcaactgta ctcgtcacta
    20101 cacgcacata caacggaggg gggctgcgat tttcaataat gtgtgatgca gatcacacaa
    20161 aacactcaat tacttaacat aaatgtataa gtcatacttt tgttttgggt gtatttcaat
    20221 ctgttaaaaa gtttttcgct acgctagcaa gcaaaaatga aacaggaata atcgaaatgg
    20281 gatgttgcgc acagttaaaa taactcaccg taaataatca tctgctataa ataatcactt
    20341 tcatg
//
LOCUS       DAZGXS010000078        20327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGXS010000078 DAZGXS010000000
VERSION     DAZGXS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            99..641
                     /gene="ecpR"
                     /locus_tag="ACHKG5_003011"
                     /gene_synonym="matA"
     CDS             99..641
                     /gene="ecpR"
                     /locus_tag="ACHKG5_003011"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP0869072.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            716..1303
                     /gene="ecpA"
                     /locus_tag="ACHKG5_003012"
                     /gene_synonym="matB"
     CDS             716..1303
                     /gene="ecpA"
                     /locus_tag="ACHKG5_003012"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP0869073.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1361..2029
                     /gene="ecpB"
                     /locus_tag="ACHKG5_003013"
                     /gene_synonym="yagY"
     CDS             1361..2029
                     /gene="ecpB"
                     /locus_tag="ACHKG5_003013"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP0869074.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2055..4580
                     /gene="ecpC"
                     /locus_tag="ACHKG5_003014"
                     /gene_synonym="yagX"
     CDS             2055..4580
                     /gene="ecpC"
                     /locus_tag="ACHKG5_003014"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP0869075.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4570..6213
                     /gene="ecpD"
                     /locus_tag="ACHKG5_003015"
     CDS             4570..6213
                     /gene="ecpD"
                     /locus_tag="ACHKG5_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP0869076.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6182..6892
                     /gene="ecpE"
                     /locus_tag="ACHKG5_003016"
     CDS             6182..6892
                     /gene="ecpE"
                     /locus_tag="ACHKG5_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP0869077.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7205..7534
                     /gene="ykgJ"
                     /locus_tag="ACHKG5_003017"
     CDS             7205..7534
                     /gene="ykgJ"
                     /locus_tag="ACHKG5_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0869078.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7782..8396)
                     /gene="yagU"
                     /locus_tag="ACHKG5_003018"
     CDS             complement(7782..8396)
                     /gene="yagU"
                     /locus_tag="ACHKG5_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0869079.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8814..9503
                     /gene="paoA"
                     /locus_tag="ACHKG5_003019"
     CDS             8814..9503
                     /gene="paoA"
                     /locus_tag="ACHKG5_003019"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP0869080.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9500..10456
                     /gene="paoB"
                     /locus_tag="ACHKG5_003020"
     CDS             9500..10456
                     /gene="paoB"
                     /locus_tag="ACHKG5_003020"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP0869081.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10453..12651
                     /gene="paoC"
                     /locus_tag="ACHKG5_003021"
     CDS             10453..12651
                     /gene="paoC"
                     /locus_tag="ACHKG5_003021"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0869082.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12661..13617
                     /gene="paoD"
                     /locus_tag="ACHKG5_003022"
     CDS             12661..13617
                     /gene="paoD"
                     /locus_tag="ACHKG5_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP0869083.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13796..14923)
                     /locus_tag="ACHKG5_003023"
     CDS             complement(13796..14923)
                     /locus_tag="ACHKG5_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0869084.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15065..16123)
                     /locus_tag="ACHKG5_003024"
     CDS             complement(15065..16123)
                     /locus_tag="ACHKG5_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0869085.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16369..17271
                     /locus_tag="ACHKG5_003025"
     CDS             16369..17271
                     /locus_tag="ACHKG5_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0869086.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            17508..17570
                     /locus_tag="ACHKG5_003026"
     CDS             17508..17570
                     /locus_tag="ACHKG5_003026"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869087.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(17974..18252)
                     /locus_tag="ACHKG5_003027"
     CDS             complement(17974..18252)
                     /locus_tag="ACHKG5_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869088.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(18419..19141)
                     /locus_tag="ACHKG5_003028"
     CDS             complement(18419..19141)
                     /locus_tag="ACHKG5_003028"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0869089.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            19240..20139
                     /locus_tag="ACHKG5_003029"
     CDS             19240..20139
                     /locus_tag="ACHKG5_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0869090.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
BASE COUNT         5133 a         5357 c         5241 g         4596 t
ORIGIN      
        1 tttactaaag aaactgaatg tacctgtaaa aattacaggt ttggaaagta gtgacatggc
       61 aaaatgatta cagcagggac tatgaggttg aaaaccatat ggaatgtcaa aaccgttctg
      121 ataaatacat ctggtctccc catgacgcct acttctataa aggactatct gaactgattg
      181 tggatatcga cagattaatt tatctatcgc tggagaaaat cagaaaagat ttcgtgttta
      241 tcaatctcaa tacggattct ttaactgaat ttataaaccg tgataatgag tggttatccg
      301 cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc ttagcaaatt
      361 attggtatta caacagcaat attaggggcg tggtatacgc tggactgagt cgtgatatta
      421 gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt aagaaagata
      481 aaatcactga ccgggaaatg gaaattatcc gcatgacggc tcagggaatg ctgcctaaat
      541 cgattgccag aattgaaaat tgtagtgtaa agacagtgta tacccatcgg cgtaatgcag
      601 aggccaagct gtactcaaaa ttatataagt tggttcagta aactccaggc aagttagttt
      661 taaaaaatga ctcactggga catcacgtcc tcaattcaac tcgggaagaa atacaatgaa
      721 aaaaaaggtt ctggcaatag ctctggtaac ggtgtttacc ggcatgggtg tggcgcaggc
      781 tgctgacgta acagctcagg ctgtagcgac ctggtcggca acagccaaaa aagacaccac
      841 cagtaagctg gttgtgacgc cactcggtag cctggcgttc cagtatgccg aaggcattaa
      901 aggttttaac tcacagaaag gtctatttga cgtggctatc gagggtgact caacggctac
      961 cgcctttaaa ctgacctcac gtcttatcac caacacatta acccagttgg atacctcagg
     1021 ttccacactg aatgtgggcg tggattataa cggcgcggca gtcgaaaaaa ctggcgatac
     1081 cgtgatgatc gataccgcca acggcgtact gggcggcaac cttagcccgc tggcaaacgg
     1141 ttacaatgcc agcaatcgta ccaccgcaca ggatggtttc actttctcca tcatcagcgg
     1201 caccaccaat ggtaccaccg cagtaaccga ttacagcact ctaccggaag gcatctggag
     1261 cggcgacgtt agcgtacagt tcgacgcgac ctggaccagt taatctctct gatgtaccag
     1321 caggggtagc cccccagctt tattcctgga cggaattgtt atgaaaaagc accttctgct
     1381 tctcgctctg ctgttatccg gaatatctcc ggcccaggcg ctggatgtcg gcgatatatc
     1441 atcgtttatg aacagtgaca gcagcacgct gagcaaaacg atcaaaaaca gtaccgacag
     1501 tggtcgcctt atcaatatcc gtctcgaacg gctctcttca ccgcttgacg acgggcaggt
     1561 tatctcaatg gacaagccgg acgagttgct actcactccc gccagcttgc tgctacccgc
     1621 ccaagccagc gaagtgatcc gcttcttcta taagggacca gcagatgaaa aagagcgcta
     1681 ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata atgccaaccg
     1741 cagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg ccccccgcca
     1801 ggcaaactac cactttcagt acgccaacgg caccctgaca aatacaggaa atgcgacgct
     1861 gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaagagt gtaaagagaa
     1921 ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg cggataacaa
     1981 aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga tagctgtgca
     2041 gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc cagtttgcct
     2101 tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata agtgcgcagc
     2161 aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac ggcatgagcg
     2221 tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag cgaatcggca
     2281 gcgcttttat ctggttggat gatggacagc tacgcatccg gaaaatacag ctggaagaga
     2341 gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatggct ctggcgaacg
     2401 ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagctg gatctcagct
     2461 tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcact gtactacgct
     2521 cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat ctgagctata
     2581 acttcggcat ctataacaac cagttgcgta acggcgggag caacacatcc agctatctgt
     2641 cgctgaataa cgttactgca ctgcgcgaac atcatgtggt gctcgacggc tcgctgtacg
     2701 ggatcggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa cgcgattttg
     2761 ccggtcaccg atttgccggt ggaacgctcg acacctggaa cttgcagtcc ttagggccga
     2821 tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag gccagctcca
     2881 ccatcttcga cagcagccag tcagccacgc cagtgatcgc ctttttaccg gcggcgggtg
     2941 aagtacatct cacccgtgat gggcggttac taagcgttca gaacttcacc atgggcaatc
     3001 atgaagtgga tacccggggt ctaccatacg gtatttacga tgtggaagtt gaggtgatcg
     3061 ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt agccgggggc
     3121 gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg tagctttcat atggatcgct
     3181 ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag ttggctggca ggtgcctcga
     3241 cctccggctc actgagtacg cttagctggg cggcaacggg atatggatac gataatcagg
     3301 cggtgggtga aacccgtctg acgctgccgc ttgggggagc gatcaacgtt aacctgcaaa
     3361 atatgctggc cagtgacagc tcatggagca gcatcggcag catcagcgcc actctaccgg
     3421 gaggctttag ttcgctgtgg gttaatcagg aaaaaacccg cattggcaat caattgcgac
     3481 gtagcgatgc cgacaaccgt gctatcggcg gcacactcaa cctgaactca ctgtggtcga
     3541 agctgggcac attcagcatc agctacaatg atgaccgccg ttacaacagc cattattaca
     3601 cggcagatta ctatcaaaat gtctacagcg gtacctttgg ttcgcttggc ctgcgggccg
     3661 gtattcagcg ctataacaac ggcgacagca acgccaatac agggaaatat atcgctctcg
     3721 atctctcgct accactgggc aactggttta gcgcagggat gactcatcaa aacggctaca
     3781 ccatggcaaa cctgtcagca cgcaagcagt ttgatgaagg gaccattcgc actgttggtg
     3841 ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaactctc agtggtgggg
     3901 cgtatgcaca gttcgacgct cgctacgcca gcggaacgct gaacgtcaat agcgcggcgg
     3961 acggctacgt caataccaat ttaaccgcca atggcagcgt cggctggcag ggtaaaaaca
     4021 ttgctgccag cgggcggact gatggcaacg ctggggtgat attcaacacc gggctggagg
     4081 acgacggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac ggcaagcgta
     4141 actatctccc gctctctccc tatggaagat atgaggtgga gttacagaac agcaaaaact
     4201 cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc tatccaggca
     4261 atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt cgtatccgtg
     4321 cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc cgaacccgaa
     4381 ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact atcgattttc
     4441 gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg cgcggtgccg
     4501 tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg acgcagacag
     4561 gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct aatctggcca
     4621 gtaactgcgc tcagagcggc agtgagcaaa acaacctggg cggatgcacc ggcacgcgag
     4681 tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg cggggcgctg
     4741 gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg ttcaggaacc
     4801 atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct ttataccaac
     4861 tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac tggcttgcgc
     4921 tgcatcaact ggtacgctgg gtgtaatatg accaccagtc ttatcctgcc gcaaaccacc
     4981 gacaccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg gatgcacggc
     5041 atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag cagctttaca
     5101 atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg cgcacgctgt
     5161 aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa agcagcaaat
     5221 ctacggttga taaataccca ctcgctggcg gaagtattta tcaacagcga cggagtaccg
     5281 actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggcagccg ttcaggatta
     5341 agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac ctcaatccat
     5401 atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta cgatatgcag
     5461 ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta ctacaccttc
     5521 aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt ctttaagcag
     5581 atggtgaacc agggaatcag cgatatcaac accaaagatc tattcaactt tcgctttcag
     5641 aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac gctgattatc
     5701 aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac accgtcgcgg
     5761 gagggatatg tcggcagcgg cgagtcggca ctcgatttcg gctatatcgt aaccaccagc
     5821 ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca ggtgattggc
     5881 gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc gttcccggcg
     5941 acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg gtgcgatgat
     6001 agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga tatctctggc
     6061 gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga taacgccatt
     6121 tctctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc aggagaaatt
     6181 catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagcgg tctgtatgct
     6241 ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac tgccgtccga
     6301 gactgacttt gtcagcaaac gtgtagtgaa taacaacaaa agcgcgcgga tataccgtat
     6361 tgccatcagt gctatcgata gcccgggcag cagtgaatta cgcacccgac cggtggatgg
     6421 tgaactgctt ttcgcccccc gccagctggc gttgcaggct ggtgagagcg agtattttaa
     6481 attttactat catgggccac aggataaccg cgagcgctac taccgggtct catttcgcga
     6541 ggtccccact cgtaacctga caaaacgcag ccctaccggc ggtgaggtta gcacggagcc
     6601 ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt ttaaatggtc
     6661 cttcgatcag gtgaccggaa cggtaagtaa caccggcaac acgtggttta agctactgat
     6721 taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac gtccggaaga
     6781 cgtggttcat cagcctgagt tacgtcagcc ggggaatcac tatctggtct ataacgacaa
     6841 attcattaag attagcgatt cctgtccggc taagccccct tcggcggact gatacttatc
     6901 catcggtcga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat gatgtggaag
     6961 cgatcgtcaa tatcacctgt aacgataggc tttctcatat gcacaccaca gtgttcagga
     7021 gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt cttcactaat taggacgtaa
     7081 aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagtacac cagccaaccg
     7141 cgatacgtcc ctgcatacga cgtaccgcag ttctctttac gatttatagc tgtactggtg
     7201 aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt
     7261 ctctttctac tgggccgaag ccgacgatgc tggcggcact attcccgcca ggctcactga
     7321 acaaatatcc ccttttcacc gatgcatgag cggaactaat cagaaaaacc cccgatgtat
     7381 tgcccttgca ggaaccccgg gcaaaaatgc ctgctgcacg atatataaaa atcgatcgtc
     7441 cacatgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg cttgcaatcg
     7501 tgcaagggct aaatacgggc tgacaccgtt ataaatatac aataattatt tgcactgccc
     7561 cgccagctgt aatagcgggg cttcgttttt caggggtaac aaaccccgat acttcttcta
     7621 tttgccagca acaatgcctc tcttctgtag cgttcttcgg atctacatca tcctgaagta
     7681 gcgcgatata ctcactgact atctgaatca gaatataaaa agcaatgttt ttaacctata
     7741 aaaatggcgc tgtatttgcg ccatttttat cattcaatgc attatctgtt tgagcctaaa
     7801 gggatctcag ggtctggctc atgagtaatt ctgtttcgta aatctctgcg aataatttca
     7861 atggaccaga accagactaa atgtccaaaa atttcagaaa cattctcata ccacgggaga
     7921 tcaaacagag gtggtgtcag tcccatgaga gggaatgaaa tcatatgaac aaaaagttgg
     7981 gctaaagcac ctgccagtaa gccctgccag agtttaattt ttggaaatac ttctgcgacc
     8041 acacaataac cgacagcaaa cactatcgaa aagataatgt gcgtaacacc aacccagtta
     8101 aagacatgcc cggcaaaggt ataaacagcc gcattgggat ctgtcagccc cagccagtct
     8161 cgaagaaaaa tatacggagg attgagaaaa ttacgcgagc aatcaatttg cccagcagcc
     8221 ctgattaatg attccgggcc acacgctgca ttaaacatat ccaccgggct acgtggcggc
     8281 aatggaactt cagcccccca cttcacaaat gcggaaacaa cgccagcaat cagcccaatg
     8341 aatgcagcaa gaccataacg tctgcggttc ggtggagttt gttcaaatat attcatatct
     8401 accctgcttg taccattatg ttatacacct ctttaggagt attcataaaa caaggtaaat
     8461 gtaaagaact gtattgtttt gtataacaag atggttgtct aatcaccaaa gaatataagc
     8521 accatcattt ctccctattt ttatattaga agtgacatag atttgcaggg tgatgcagag
     8581 ctgacaatca caggtttcct tatcggtttt tgcatcgtac aactaaagca ataaaccagc
     8641 tcccatcatc atcaacatcc cggcgaacat gaatttatta gccgggctca tagctgcatt
     8701 cgcgttttaa gaataatcct cctgctgtcg ccgactatgc ttaacgttta aaaaagcatc
     8761 agcactctcg caacgcactc ttattttccc ctttagaata ccggaggcct ggtatgagca
     8821 accaaggcga ataccccgaa gataatcggg ttgggaagca tgagccgcac gatttaagtt
     8881 tgacccgtcg cgatctgatt aaagtgagcg ccgcaacggc ggcggccgcc gtggtttatc
     8941 ctcattctac gcttgcggca agcgttccgg cagctacacc cgcgcctgag ataatgcccc
     9001 tgacacttaa ggtgaacggc aaaaccgagc agcttgaggt ggatacccga accacgctac
     9061 tggacgcttt gcgtgaaaat ctgcatttga tcggtaccaa gaaaggttgc gatcacggac
     9121 agtgcggagc ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc ctgacgcttg
     9181 cagtcatgca tcagggggcc gagatcacca ccattgaagg cctgggttca ccagataatc
     9241 ttcaccccat gcaggcggcc tttatcaagc atgatggctt ccagtgcggc tactgcacct
     9301 ccgggcaaat ttgctcatca gtagcggtgc taaaagagat tcaggacggc attcccagtc
     9361 acgtcacggt cgatttggtt tcccctccag aaagaactgc cgatgagatc cgtgaacgta
     9421 tgagcggcaa catctgtcgc tgtggtgcat acgctaacat ccttgccgcc attgaagacg
     9481 ctgcggggga gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca cccccgccga
     9541 ggcggcactt agcgctcagc gcgtacccgg cgcaaaattt atcgcgggcg ggaccaatct
     9601 gctggacctg atgaagctgg aaattgaaac gcccacccac cttatcgatg tgaacgggct
     9661 cgggctcgat aagatcgaag tgaccgacgc gggtgggctg cgcatcggcg cactggtacg
     9721 gaacaccgac ctggtggctc acgagcgcgt gcgtcgtgat tacgcggtac tctcccgcgc
     9781 cctgctcgct ggcgcgtctg gtcagttacg caatcaggca accacagcag gtaatctgct
     9841 ccagcgcacg cgctgcccct atttttacga caccaatcag ccctgcaata agcgcctgcc
     9901 cgggagcggc tgcgcggcgc ttgaaggctt tagccgtcag cacgcggtgg taggcgtaag
     9961 cgaagcctgc attgccaccc atccgagcga tatggcggtc gcaatgcggt tgctggatgc
    10021 ggtggtggaa accatcacgc cggagggaaa gactcgcagt atcacactgg ctgattttta
    10081 tcacccaccg gggaaaacgc cgcacattga aaccgccctg cttcccggtg agcttatcgt
    10141 tgcggtgacg ttacctccgc cgctcggcgg aaaacatatc taccgtaagg tgcgcgatcg
    10201 cgcctcctac gcctttgccc tggtatcggt cgcggcgatt attcagcctg acggcagcgg
    10261 gcgcgtcgcg ctgggcggag tagcacataa gccctggcgc attgaggctg cggatgctca
    10321 gctatcccag ggggcgcagg ccgtatatga cgcgctgttc gccagcgccc atcccaccgc
    10381 tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac tggctgaagc
    10441 gagggcacaa gcatgaaatt tgataaaccc gcaggggaaa acccgatcga tcagctgaag
    10501 gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg cacggcacgc
    10561 tacgcctacg aatggcatga agaatccccc aacgccgcct atggctatat cgtcggttcc
    10621 gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa agcgccgggc
    10681 gtactgcctg tcattaccgc cagtaacgcc ggggcactca gtaaaggcga caaaaacacc
    10741 gccaggctgt taggcggccc cactattgag cactatcatc aggccattgc tctggtagtg
    10801 gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcgca ctatcgccgt
    10861 aataaaggag cttactccct ggcggacgaa aaacaggccg tcagtcagcc gccggaagac
    10921 acgcccgaca agaacgtcgg tgactttgac ggggctttct cctccgctgc ggtgaagatt
    10981 gatgctacct acacgacccc ggaccagagc catatggcga tggagccgca tgcctcgatg
    11041 gccgtctggg atggaaataa gcttactctc tggacctcaa atcagatgat tgactggtgc
    11101 cgcaccgatc tggcaaaaac gctgaaagtg cccgtggaga atgtgcgtat tatctccccg
    11161 tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct gctggcggcc
    11221 ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc ctctattccc
    11281 aataacacca cgcaccgccc cgccaccctt cagcacttgc gtatcggtgc cgaccagagc
    11341 gggaaaatca ccgctatctc acatgaaagc tggtctggaa acctgcccgg cggcacgccg
    11401 gaaacggcgg tacagcaaag cgaattactc tacgccgggg caaaccgtca taccggcctg
    11461 cggctcgcca cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc
    11521 ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg catcgatccc
    11581 gtcgagtttc gcatcctgaa tgacactcag attgaccccg ccgacccgac gcgccgcttc
    11641 tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg ttggaagcag
    11701 cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc tagtcggcca cggcgtcgcg
    11761 gcgggctttc gcaataatct gctggaaaaa tcgggggctc gggttcacct cgaaccaaac
    11821 ggcaccgtta ccgtggaaac ggacatgacc gacattggca ccggcagcta caccattctg
    11881 gcccagacgg cagcggaaat gcttggcgta ccgctggagc aggttgcggt tcacctcggc
    11941 gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa tacctccacc
    12001 tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc agtcgggttt
    12061 gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg aagcgccata
    12121 ctacatgagg ccaccgcagg cggcagactg acagcggaag agagcattga attcggaaca
    12181 ctgagcaagg agtaccagca gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat
    12241 agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc
    12301 ctgaatccga aaactgcacg cagccaagtc attggcgcaa tgactatggg catgggcgcg
    12361 gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa tcacgatatg
    12421 gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaagtgat tttcctggat
    12481 gataccgacc ccatatcctc cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc
    12541 ggcgtgagcg cggctatcgc caacgcggtg tataacgcca ccggtattcg ggtacgcgat
    12601 tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta aggaggaaca
    12661 atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca ggcgtttctc
    12721 accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga
    12781 gcggtgctgg tgacgctggt ggagatacgc ggcggcgcgg cgcgcccgct cggggcgcag
    12841 atggtggtgc gcgaagatgg tcgttactgc ggttttgtct ctggcggctg cgtggaggcc
    12901 gctgccgctt ttgaagcgct ggagatgatg ggctcaggct gcgatcgcga aattcgctat
    12961 ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat cacgctgacg
    13021 ctccataaac tacgctcggc acagcctctg ctcgccgtgc tgaaccgcct ggaacagaga
    13081 aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct gcccacgcaa
    13141 acccgaacgg gctggaatct caatggcttt gaggtggggt tcaggccatg cgtcaggctg
    13201 atgatttacg gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc
    13261 tatgacagcc atatcttcga tctttttccg gcctcagcca gcgctcagat cgataccgat
    13321 acggcggtca ttttgctgtg ccatgatctc aaccgggagc tgccagtgtt gcaggccgcg
    13381 cgagaagcaa aaccctttta tctcggcgca ttgggcagct atcgaaccca cacgttacgt
    13441 ctgcaaaagc tccacgagct gggatggtcc agggaggaga cagcgcaaat ccgggcaccc
    13501 gtcgggatat tccccaaagc ccggaatgcg catacactgg ccctctccgt gctggcagaa
    13561 gtcgcctctg tacgtctcca tcaggaggag gattcatgcc tgcccccgtc gtcctgatcc
    13621 ttgcggccgg gcgtggaaag cgctttctca cctccggggg aaataccata aattaagaga
    13681 aaaatttgca gtagccgttt tagaaaatca cctaaatcgt ttaaaagagc gccctaccca
    13741 tagtggttgc tcatcgcctt gtgtaacttt ttcagcaacg cgcgccctcg ccggactaaa
    13801 gcgcaccggc cttctggcgt gctgtaacaa aggtcagtac gcccgcgcaa aggagcagca
    13861 cactactcat ggcaaacgta ctctgccagc caaggcggtc aaacacgatg cctcctgccg
    13921 ttgatccaag ggcgattgag agctgaatca ccgcgaccat gagcccgcct ccggcttcgg
    13981 cgttgtcagg caatgtacgc gcaatccatg tccaccatcc ggttggcgcg gcggtggcca
    14041 gcatgcccca gagtcctaac agcagcgaga ccatccagac gtgatgcccg gtaagcatca
    14101 gaccgatggc aatgacggcc atcagtccgg gtatggccat cagcgtgggg tagaattttc
    14161 tgttgagaaa tgtcgaaaca accagcgtgc caataaaacc ggccacgccg ataatcagca
    14221 aaatcaacga caagcctaag gaattaaccc gtgtcaccga ctccagaaat gggcgcacat
    14281 aggtgaatag cgcaaactgc cccatgaaaa acaggccaca agctatcatg ccgaccacaa
    14341 ccatgcggcg gctaaataag cggaataccg cgccacatga cgtgtggctt ttattcgcat
    14401 ccatgctggg cagactaatg cactgccaga taaaggcgac aaccgccatg ggcaccaagc
    14461 acaagaaagc gcctcgccat ccgaccgtag cccccagata gcttcccagc ggcgcggcca
    14521 cgaccgtcgc cagcgcatta ccggcgttga aaatcgccag cgcacgcgtg acctgatgct
    14581 gcggcaccag acgaatcgcc gttgccgctg acatcgacca gaatccgcca atggccacac
    14641 caatcatcgc acgaccgacc atatacatca gataactggt agccagcgca ataataagcc
    14701 ccgatacggc catcagaacc gtcatcccca gcagcaggaa cttacgattc atctttccag
    14761 ctagcgttga aagcgtcagg ctggtcagaa ccgccagcgc accggagata gcaatccccc
    14821 gccctgccag cccctccgtc acgcccaggt cacgggcaat cggcgtgagc aggctgacgg
    14881 gcataaattc agaagcaatc agcacaaaca cgcacagggt catggcaaaa ataccgcccc
    14941 agtatgcgtg ctgatgatga ggtgatggtg tttggcttag tgtcgacata gtgaaatttc
    15001 acaaagatca ggataaaagc caccggccat agaccggtgg ctttgcatca attcagggga
    15061 ggttttattt cagtttggtc tggaaaaatt gttcgaactt cgcgaaaggt atttttccgg
    15121 ccacgttatc gtacaggtcc acgtgatttg cccctggtac caccaccaac tctttgtctt
    15181 tactgccaac cgccttgtaa gcatcttcag caaaatagcg tgaatgtgct ttctcaccgg
    15241 tcacaatcag cgtcggaatg gtgatttcac tggcgtagct caacagcggc atattcataa
    15301 atgacagcgg catcgttgcc gtccacgccc cggtagagtt aacggaacgt tcgtggaagc
    15361 cgcgcggcat gcgatagtag tcaaagaact ctttcagtac cggatgagga tttgccggca
    15421 gcgtttctgg cagaatgcgc tcgcccgcac tgaccttgcc gttgctatcg acattaatat
    15481 catgaccacc gtgagcgaac gttccgctct gcgcatcctt ccagcgctgt tcattcaaat
    15541 attgcagaac agcacgacgg tcggcggtag aataacggtc ttcgccatcc cccacaccat
    15601 ggcccatcgc ccggctcatg tcatacatca cgctggtagc aaccgcttta acgcgggtat
    15661 ccatcgccgc gtcgttcagc gccatgccgc cccagccaca aataccgagc agcccgatac
    15721 ggttgcgatc tacctctttt tgcagcccta agaaatccac cgccgcgctg aaatcttcag
    15781 tgttgatatc cggtgaggcg acatttcgcg gatagccgcc gctttccccg gtgtaagaag
    15841 gatcgaacgc cagggtaaca aaccctcgtt ccgccagcgt ctgcgcatac aagccgctgg
    15901 attgctcttt caccgcgcca aatggcccac tgaccgcaat cgccgccagc ttgcgatcgc
    15961 cacggtcttt aggcaggtaa agatcgccca ctaaggtgat gccatatcgg ttcgggaacg
    16021 agactttacg gtgatcgact ttctggctct cggcgaacgt tttatcccat ttatcggtca
    16081 tggacacagg ggcattagga ttggttgaat cagcataact cattgtcgtg actccactta
    16141 atgatgcgaa aagcagcatg accggcatcg cggtttttag cgttcgggtg aaggctttca
    16201 taaaaatccc cataaaaaac aacagagaga tacaggtttc cgacggacaa cgctgcggtg
    16261 ctttactttg atggggtcat tatagtgagc agaattagtg ctgattaggg ggcgattaat
    16321 gctaagatta ataccatatt gttataaatt aatgctgaga ttgccggcat gaaggggaat
    16381 gtttcaatgg caaaaaggga gaactacaac gagctatacc tgtttatgca ggtggtgcgg
    16441 gaaggcagct ttaccgcagc ggctaagcgg ctgggtcttg ctcagtcagg agtcagccgt
    16501 tctgttcgcg agcttgaaga aaggctgggc gtccagctgc tggtgcgcac cacgcgtaaa
    16561 ctgtcgctga cgcaagcagg cgagcagctc taccagaaga cggcatctgg atttgaaatg
    16621 ttagatcttg ggcttgccac gctggcgcac tatcgagaga cgccctccgg cacggtgcgt
    16681 atcaatgcca gccagcacgc gattgataaa tgcctgctgc caaagctggc ggtatttaaa
    16741 cagcgatatc ctgatatcca gcttgaactg ataaatgaga gccggttcgt cgatatcatc
    16801 gaggaaagat tcgatgcagg tgtccgcctg gggcctgaag tcagccaggg catggtcgcg
    16861 gtacgcatca cgcccgatat ggagatggca attgtgggga ctccggaaca ttttcgtcgc
    16921 tatggttttc cgcaaacccc ggcggattta aaggcgcatc cctgtatcgc ctatcagttt
    16981 gccgacggca gcgtatacca gtgggaactc aatcaggatg ataaaaaaat cactcatcaa
    17041 cccgaaggac aatgggcctt ttccgacagc tatatggaag cagaagccgc ccggctgggc
    17101 ctcgggttgg cctatgttcc tgttgaactg gtcgcggatg atttagaaca cggtaagctt
    17161 atcagagtgt tgcagcgtta cagcctgcga atggagggat tgtttctcta ttaccctcat
    17221 cgcaacgtct cccccgctct gcggatggtc attgatacat tgaaaatctg aacgttaaca
    17281 acgaccgact cttcactgtg caatattgtg tcaggtagtc actctgcgtt caattccagg
    17341 ggccgctttg agcgagaaag agcggactta tctgtacaag gggactatcc agcaatggag
    17401 atcatccaaa agaaaaaacc acctggctcc cctcgacaac cttaatccgg gcatttagcc
    17461 tcaatcaaca ttttcatatc tgtcaggtta cttcattatg aaaaccgatc aataccgttg
    17521 tttttgttgc aacagtagaa tacgcgtatt tatccctcca tttctattaa taacggcaat
    17581 aaactgttca tttcactgat atttaaaata tgcatccttt tcaagctgtt cagcggcgta
    17641 agaaaacgta ttaaatatga attcgacgat ttaaccattt ctgtctgatt ggtaatcatc
    17701 tgtgattaat gctggtctat tgattactcc agcattaacc gcttagggat gtattcttcc
    17761 tgttgatata cagccctcga aaaagagaga aaataaaact gtgtccaata ttactctacc
    17821 actccagacc gaagcatcgg tagaattagc agaacgactg gtacaataga ttgggatagc
    17881 gtcatttgtc aggtattcca ttgcctgaca aatgacaatt ttgagtcaat agacgtgctt
    17941 cttgatcatg tcttaaaaat tataccatga gccttatgaa gcatcatttt tactgactgg
    18001 tgttaacttt gctatcatat aaactctgtt cttaaaaaac gcgcccagaa ttttatatga
    18061 tttcccttgt ttttctgcct gtaaagaaat ttctttttca gtgctgttaa tggttgaacc
    18121 gacggcagta atcgttttgg cgacgctact gaatgataat aaagacagtg caaccacaac
    18181 aattaaagaa cagatagtat tcacgctaat ttccctgaca attgagagga tgagttttat
    18241 atataaagac attaaacatg agtcatgttt acttcctgtg aaaaataatc tttctgtaat
    18301 taaaccttca tctccttcgg ggtgagcaat acataatgta tcttgctatg aaaaacacag
    18361 ggatatataa atattccttc tgacctattt aataaaacgt gaatgtaaaa gttattcttc
    18421 agtttgcaga tgcagtgggt ctgatggtaa tttcggttgt atcaacgctt tcgggagatt
    18481 caataatatg ccttacagca cgggcaatat ccgcgggttg aagtgcaaca gaacggtagg
    18541 cgtccattgc cttcatggtt tccacatggg taatggttga agccagttca ctttcgacaa
    18601 cgccaggatt tacgcaggtc acacggattt tcgagctttc ctgccgtaag ccatcagaaa
    18661 tagcccgtac tgcaaatttt gatgcacaat agactgcgcc tgtgggcaca acagaaaggg
    18721 caccaataga accaagattg attatctgac cggaaccctg tgcttccatc accggaagta
    18781 cagcgccaat tccccacagt acacctttga tattcacgtc aatagtgagc gcccattcat
    18841 cttgttttcc tgctgcaagc ggtgaaagcg gcataacgcc cgcattattg ataagaacat
    18901 caactcgccc ccagctatcc agcgctgctt gcacgaaatc ggccatggac tgtcggtctg
    18961 tgacatccaa ctcccgcgct ttagcaattc ctcctgcgcg gcagatttcg gttgcaatgg
    19021 cttcgatacg ctcaactctg cgtgctccca gtaaaacctt tgcacctgtc attccaagct
    19081 ctctggcaat accttcccca ataccacttg atgcgccggt aattaatatg accttatcca
    19141 ttacgttctc cttcaaatgt gttcggagag agaatagaac tttatgattg gttccagtat
    19201 cctgctttca atgtactcag tggttagcgg cattaaacaa tgaaaatcga tcttaatctt
    19261 ttacccattt ttatcgcggt agcagaagag agtaatttta gtaaagccgc cgcacggctg
    19321 ggcgttaccc gttcagcggt cagtcaggga ataagacgcc ttgaagatgc attcgggaca
    19381 atgcttgtaa tgcggaccac gcgctcagtt aatttgacag aagcgggaga acggctgcat
    19441 aaatcgctgt cattaccttt atccggtatt gaggcagcgt tcgaagagat ggtttctgac
    19501 catgtgccac gcggacttct cagaatcgcg gtgacgtcaa tagctgaaga atttctttca
    19561 ggtccgctaa tcgcttcgtt tgcagcagcc aatccagccg ttacgcttga tattgttgtc
    19621 acggatgagg aattcgatat cgtggccgca ggttacgatg ctggcgtaag gctcggagag
    19681 gtcattgaaa aagacatgat tgctgtgccc cttaccggca ggcagcgtga aatggtcgtt
    19741 gcctctccct cctatctcgc agccaacagc acacctgttc atccgcgtga acttgtgaat
    19801 cataagtgta tcggctggcg tcagtccccg gaggttgcgc cttatcgctg gccatttgaa
    19861 gaaaacggga gagctttcga ccttgcgatt gaaccgcaga ttacgactaa tgatctgcgt
    19921 ctgatgttga ggctggctct tgccggcgga ggaataacaa ttgccactca ggaaactttc
    19981 aggccgtata ttgaaagcgg taagcttgta tctctgcttg atgactttct tccacaattt
    20041 ccgggcttct atctgtattt cccacagcgt cgcaatattg caccaaagct ccgcgctctg
    20101 attgaccacg ttaaggaatg gcggcagcaa ttggcttaaa tgtctgcacc tgcattgcct
    20161 gatgtcagaa cagtattttg attaattgcc agggttacaa tggcacaaat gcggcacagg
    20221 aggaaacgtg gtgtatttaa atatggggta actacttgat ttaaatggtg cgataatagg
    20281 aaccaataat cgccaccaac acaatgattt taatacataa attaaaa
//
LOCUS       DAZGXS010000079        20022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGXS010000079 DAZGXS010000000
VERSION     DAZGXS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(688..1119)
                     /locus_tag="ACHKG5_003030"
     CDS             complement(688..1119)
                     /locus_tag="ACHKG5_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP0869091.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(1187..3208)
                     /locus_tag="ACHKG5_003031"
                     /pseudo
     CDS             complement(1187..3208)
                     /locus_tag="ACHKG5_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3342..3467)
                     /locus_tag="ACHKG5_003032"
     CDS             complement(3342..3467)
                     /locus_tag="ACHKG5_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP0869092.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVVAFMAYESGK"
     gene            complement(3409..3558)
                     /locus_tag="ACHKG5_003033"
                     /pseudo
     CDS             complement(3409..3558)
                     /locus_tag="ACHKG5_003033"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3732..4493)
                     /locus_tag="ACHKG5_003034"
     CDS             complement(3732..4493)
                     /locus_tag="ACHKG5_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0869093.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(4490..4924)
                     /gene="psiB"
                     /locus_tag="ACHKG5_003035"
     CDS             complement(4490..4924)
                     /gene="psiB"
                     /locus_tag="ACHKG5_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0869094.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4979..6937)
                     /locus_tag="ACHKG5_003036"
     CDS             complement(4979..6937)
                     /locus_tag="ACHKG5_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0869095.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(7003..7236)
                     /locus_tag="ACHKG5_003037"
     CDS             complement(7003..7236)
                     /locus_tag="ACHKG5_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0869096.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(7293..7745)
                     /locus_tag="ACHKG5_003038"
     CDS             complement(7293..7745)
                     /locus_tag="ACHKG5_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0869097.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7771..7977)
                     /locus_tag="ACHKG5_003039"
     CDS             complement(7771..7977)
                     /locus_tag="ACHKG5_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0869098.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            8140..8520
                     /locus_tag="ACHKG5_003040"
     CDS             8140..8520
                     /locus_tag="ACHKG5_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HFP0869099.1"
                     /translation="MPGCRERRPRRDRGGATGTVAASPGFASGMEARQGGDSSAGSVH
                     DSPPRQGDARKRHKQENNKTDSNQSSRKKKNSSSSSSSSSSSSSSSSSSSSMNNNIIS
                     NRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(8606..9169)
                     /locus_tag="ACHKG5_003041"
     CDS             complement(8606..9169)
                     /locus_tag="ACHKG5_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP0869100.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(9216..10577)
                     /locus_tag="ACHKG5_003042"
     CDS             complement(9216..10577)
                     /locus_tag="ACHKG5_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP0869101.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10629..10859)
                     /locus_tag="ACHKG5_003043"
     CDS             complement(10629..10859)
                     /locus_tag="ACHKG5_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869102.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            11376..11885
                     /locus_tag="ACHKG5_003044"
     CDS             11376..11885
                     /locus_tag="ACHKG5_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869103.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(11855..12046)
                     /locus_tag="ACHKG5_003045"
     CDS             complement(11855..12046)
                     /locus_tag="ACHKG5_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869104.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(12043..12465)
                     /locus_tag="ACHKG5_003046"
     CDS             complement(12043..12465)
                     /locus_tag="ACHKG5_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0869105.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12512..12937)
                     /locus_tag="ACHKG5_003047"
     CDS             complement(12512..12937)
                     /locus_tag="ACHKG5_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0869106.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(12903..13147)
                     /locus_tag="ACHKG5_003048"
                     /pseudo
     CDS             complement(12903..13147)
                     /locus_tag="ACHKG5_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315569.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13122..13898)
                     /locus_tag="ACHKG5_003049"
     CDS             complement(13122..13898)
                     /locus_tag="ACHKG5_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869107.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(13944..14498)
                     /locus_tag="ACHKG5_003050"
     CDS             complement(13944..14498)
                     /locus_tag="ACHKG5_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0869108.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(14392..14613)
                     /locus_tag="ACHKG5_003051"
     CDS             complement(14392..14613)
                     /locus_tag="ACHKG5_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869109.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(14614..15297)
                     /locus_tag="ACHKG5_003052"
     CDS             complement(14614..15297)
                     /locus_tag="ACHKG5_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP0869110.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15374..15679)
                     /locus_tag="ACHKG5_003053"
     CDS             complement(15374..15679)
                     /locus_tag="ACHKG5_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869111.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(15683..16585)
                     /locus_tag="ACHKG5_003054"
     CDS             complement(15683..16585)
                     /locus_tag="ACHKG5_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0869112.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(17280..18251)
                     /locus_tag="ACHKG5_003055"
     CDS             complement(17280..18251)
                     /locus_tag="ACHKG5_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFP0869113.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(18251..19417)
                     /gene="sopA"
                     /locus_tag="ACHKG5_003056"
     CDS             complement(18251..19417)
                     /gene="sopA"
                     /locus_tag="ACHKG5_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFP0869114.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
BASE COUNT         4562 a         5889 c         5006 g         4565 t
ORIGIN      
        1 tatttacgta cggatgaagg ctggccgtat ctggctgtgg tcacgtgcca ttattggctg
       61 atcaatgtcg tcacgcatga cggcacagga gcccccggaa cgttatgcta ccgcatgtca
      121 tttgcaaaaa tgacagatag atatacttca atttgaatct taacaattag catgggaatg
      181 ttgtcaatcc tctgtatgga gccaccacat attgcaattt ttgttctctt ttaaaatata
      241 ttccacgagc gtcttgtggg accttttgct gttatagggg ggcagtttgg atatcgaaaa
      301 ttattgtacg ttaaggctga atttcaatcg caaagcataa ccttaccttt ctgcttactt
      361 atgcagttca aagagtacca actctgcaat tttcgttgcc acagatgcaa tatcgttact
      421 ttccagtcac catgccgctg aaagcccaca ccatgcgata atccacatca agagccgctt
      481 tctgtccaga cccgctgttg tgaggtgtga ggtgtgaggt gtgaggtagc ctgagtttaa
      541 cggacaggcc gatgaaggac gccgttaccg ggataatgta agaatctttt tttgagtatg
      601 tcaacataac ctgaggcaaa gtacctcagg ttccagtaat cagccgacct gacagtcttc
      661 tgttagccct acagatatgt cgtgtagcta accgcgcatg tcaaagccgg atttttgaaa
      721 atctctataa acttccggat tgttgaaggc cgggaattta gctttatggg ctgctgactt
      781 tatcgcttcg caataggctt tatcgccatt actggttgat atttttaacg ccgtgccatc
      841 ccgggagaat tccatatgca acctgcattt tttccttttc catttttgcg gatcatcaag
      901 tttagcatta attgcatccc tgattccccg tgcctgctcc ccccattcat cctgatcatc
      961 ccaacgtcct gaactgcaac tacccagggc tgtagtttta tggcaatttg aaggatgtaa
     1021 tggcgcacat cccgccacaa agctgactaa aatagccagt ataacgattt tctttacccc
     1081 catatattgc tcctcaaccc atatagatct cagtaccata gctctgtcag agcaaagaaa
     1141 atcttaacta atacaagtta agatttttaa tatccactcc tcatccctac attccaggtg
     1201 atggtggcgt ccactcatta tcctgaactg gagcataaga tgcgccatgc cccccggata
     1261 tgcttttctg ttgcctgata tattcgtggt agtactgttc actacgctga cgatgtaaat
     1321 ccacatccgg gcggctgatt acaaagtgtc catttccggt tattccagct gcattatgcc
     1381 aggccaccat ttccgcaaat acatcattaa actgttgata tgagccttcc ggttcaaaac
     1441 ccattcctgg ctcacgatca aacaatacag gagcaatcag attcagctta tccaccagtt
     1501 catttctgtc ctgatgatgg tagaacacca cacggtcccc ataatccaca cagactaact
     1561 cgccattcct ccgatcacga aaacgcacac tgccatcttt ctgcagacgg gcttcgagaa
     1621 cgccagtgtc ttcataaagt ggagttcctc ccggttcaca gagaatcacg cagcggtcgg
     1681 cattcgttgt tcgtcgcttg tctttgcgac gatcacgata atcccagcca cgtaactgtg
     1741 ataatgcagc tctgtctccc tgtaccgcct gttgctccac ccactgccgg taggacaaag
     1801 gataccactt accctcctca atcagtgaca accgctcttc cttcacactc tccttcagcc
     1861 tgatcagcgc ctgcatacgc tgcacttccg caatatggta atgcagctta cgcaattgtg
     1921 gatcccggaa ctgcacacgg atatacgcct tgcgccgacg gcaggcagca tgaatttccc
     1981 gtaaccgttc tccgtaacgt aagtcgggtt tacgccagtg ctccttccat gcaagataac
     2041 gcgcgcgcaa atcttcccgc gcagctgcac gggcttcacg gcgttcacga cgcagtacag
     2101 gatcgcgccg gaagccgggc tccacttcat cgctggcagc aagttccgga ttgtagcgac
     2161 attcaggctt cacccgttca aatatatctg cacctgcaat ctcaaatggc cctgcctgcg
     2221 gctcagccct ggataacgtc aaatccgggt ggatactgct ggccttcact ggtgtgagct
     2281 catgattaaa cgcatccaca atcacaagac catgatgctg tttctgcaac ataagccctt
     2341 tacgcgcaaa catctcatgg cacgcctgcc agctgaaatt cccgcccgcg atcatatctt
     2401 caatcaacga ctcacactga acagttgtgt acccccacag actttcctta tcagaaaaca
     2461 gttcgcgctt tattgctccc tgtggacctg tccccatccg catacgatac cattcgtaat
     2521 cactgacagc ctgagtacca agattacgtc cccggaaccg ttctccgggt ataacctgag
     2581 taaaaatatc cttcggtaca ggctgccatc caccatccag atcctgcata taatccagcg
     2641 tcattaacgg ccacacactg tctgcacgta ctggtgtctg ttgtttatca aatccatcat
     2701 gcagtaccag atgcccgtgc tggatcccca tccataatcc agccgttgcc agagttctgt
     2761 gcacatccag gcaactttcc agctcaccat ttctcgccat ttccggtaag cgttcggcaa
     2821 aatgacggca ggcgtactgt ttcagactct ccgtttctgc cactttttcc ggccggatat
     2881 caacgtttat ggcttccgga tcataacgac cgggtgtccc cacctgactg aaaatatctg
     2941 tcggtacagg ctgatattcc cccagtttcc gccccagttt ttcagctgtc cataacggtc
     3001 cgaacgaact gagtgcaaca ccttcccgcg ctctgtccca gccatccttc accaccagtt
     3061 ccccttcctg catcgtgata tacagaccat cactggccag ccgtttatgc aaagataacc
     3121 agtcctgtgc gggctcttca cgcagcccgg caatcgacat ctgagcaata tattccctga
     3181 acgtctgctt tttcccgcgt cgccaggctg ttcatgcccg tacaatgcca gcaaggagaa
     3241 aggggccacc ggcgaaccag cagccccttt ataaaggcgc ttcagtgcag tcagaccagc
     3301 atcagccctg aaaaggcggg cctgcgcccg cctccaggtt gctacttacc ggattcgtaa
     3361 gccatgaaag ccaccacctc cctgtatccg tctctgtaac gaatctcgca cagcgatttt
     3421 cgtgtcagat aagtgaatat caacagtgtg agacacacta tcaacacaca ccagacaaga
     3481 gagctgcgtg gtagtttcat ggccttcttc tccttgcgca aagcgcggta agaggctatc
     3541 ctgatgttgt ctaagcatgc aggggcctcg tgggttaatg aaaaattaac tacggggctt
     3601 ttgtccttct gccacacaac acggtaacaa accaccttca cgtcatgagg cagaaagcct
     3661 caagcgccgg gcacatcata gcccatatac cagcacgctg accacattca cttttcctga
     3721 aaataacccg ctcattcagg ctgtttacgg gcaatccgtg tgattgttgc cgcatcacgc
     3781 acctcccgga gtttgttcgg caccatccac cgctccatct cacggacagc atgaccggcc
     3841 tcctttgctg ccagactggc ttccgcgata acctgccaga atggcatatc ctgccactgc
     3901 cagcgttcca ttccggccag tgacggaaaa cgctcgcccc actgccagaa cagggtttcc
     3961 aggtcatgct ctgccacccg atcactccag cgtgcatacc agcttccgac tgtttccggt
     4021 gaatggaacg ccagttcgat ttcagcctgg gcgaccagcg cctggtacgc acgggctttc
     4081 tgctctgcct ccctcgcctg ctggcggctg tacttctgca tgaccagcgc ggatttatgg
     4141 cgactgcgtt ccctgacaca aaaccgcacc gacgggaaca agcgttctgc tgcatcaccg
     4201 ggaaggggaa ggaggcagac ctcgccctgt gtttcaatca gcacatcgat ggcatacaac
     4261 cactgaagac ggtttccgtg cagttcttct gcggtcaggg acggcatgaa aaatcgcagg
     4321 tcagacagcg atatcctgcg gcttccgttc agacagcgga aaaacgcccc cgcatacgga
     4381 tattccgcca gcgtgtttcc ctgattgcgg cgtttttccg tctctgccac tgcccgccat
     4441 gcggcctgtt gctctgtgct gaggggaacc agtgcctgtg aacgtgcgct catacctgac
     4501 ctccttccgc catcaggata tggatgatgt cgttaacgct gtatccctgc gtatccagtg
     4561 acgccgccag tgccagtgaa tggctgacgg cttccggtgc aaaccgccgc tgtacctgaa
     4621 ccaccgcaaa cggctcacca ccggcattca ccagaaccag cacccagacc tgggagacat
     4681 cgcccggtga acataacgcc aggtgaaaac gttcgtgggc aggcgtgaaa cggacctgga
     4741 cggggaaaaa gccgccaaac tcgctgccgt actcgccgtt catcgtccag ttgtccggcg
     4801 catccagctc ccgcatcacg gcgtgtgtca gctcacggcg tgcatcactt ccggcagccc
     4861 ggatatcttc atattcctgt gcgttcatgg tatgtaatac attcagagtc agttcagttt
     4921 tcatcacggt atctcccttg ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc
     4981 aggcggcatc agccaggtta tcggtgcgtt cagtggcatc cgtctgtggg cgtggtgcac
     5041 acatccagcc aggcacccag cggttgtctt tcatatggtg ctctgcatgt tcagccgcat
     5101 cgcctttctt catcttctcc gcgtcccgtg cggcaccgga tagtcctgcc tcattcaggg
     5161 ctgcgataat ctgcggcttt ttcaggtgtc cgaaaaagtt tgctttcgtc ggctgccacc
     5221 agtcgcgcat gtgaaagccg atggcgctct ccagcgtgtc cagcggactg cgtgacgtgt
     5281 gaccatactc acgggtctgg acaccgtgga tactgcacgc ggtgcagaaa ctgagcagtg
     5341 ataacagcac ctcctgactg agtgacagga acgtcgtcat atcccgtgcc catccatccg
     5401 gcagcagggc ggcaagacgg gctttttctg ccatcatcgc catgaatgcg gcaccttcct
     5461 tccctgacgg cgcatcgctg gtcagcgaat aatgctcaca ttccaggcgg attctggcgg
     5521 gattgctgta cgctccactg ccaaatacat tcagacagag tgtccatgcc agaagtgcca
     5581 gggatttgtc tgattgctgc atcagtgccg cctggactgc cagcgtgcgc tctgaggaca
     5641 ttttcgtcag cagcggcaga ctgatttcct ccactgaggc tttctcctgc acctgttccg
     5701 tgcggtaatc gttatcagcc acatcatcct cactgcgcaa ctgcacacca cgctggacac
     5761 acacgttgcc ataacgccag gacaccacca cgccacttcc ggcacgcatc tccggcgtcc
     5821 acgccctgac cttcgccatg cagcgcatca gcttcatttc ggcttccagc aggtcggatt
     5881 cctcacactg gttttccagc gcatcgtaac gtgccatcag ttcgttcagg cgttcttctt
     5941 ctgcctccgt cagcaccgct tccggctccg gcagactgcg gtatgttccg gcatcctcac
     6001 ggcagaaacc aacaggctcc atacgtccgg cgcaccattc ccagccttcg gcttcccgaa
     6061 ggtgttcggc gacagcctgg agtttttcca gcagggcggc atcgagcgcc acgcagtcca
     6121 catagccacc ctcgtcgtca ctgaacaaat cggtgcgcag ttcatctggc gagaaggtat
     6181 cagcccccac gaagcggaat ttactgttcc ctgccaccgc cacttcactt tcggtcacca
     6241 gacgacgaat ggtctgtact tccggtttac cgccccatcc cgactgacag gcggcttcaa
     6301 acacctgcac ctgacgcgcg gtgtcgttct ccagcgccag cgcctgacag tgctcggtgg
     6361 tgatgcggtc ttctgccagc gcatcgagga tgacaggcgc aaggtctgcc agtttcagca
     6421 ttcgctgaac gtggcggggc gaataaccca gcaaatcacc gatttgtgca ggtgttttgc
     6481 cttcctgcgc cattgcacgg aatccggcaa tctgttcggc agggtgcata tcccgacgat
     6541 gaccgttctc ggtcatcgat gcggcagtcg ccagctcctg cggaatgacc ttcacacgga
     6601 caggccagtc agccggaatg atgtcacgct ctgccagcat gttgagtgct gccagtcggc
     6661 gaccacctgc ggcgacaccg taacggtcac ctggcagggc atgaacaacc agattctgta
     6721 acaggccgac acctttaatg gagtccgcca gttcgctgac ggactccgca gaatacggca
     6781 ccgtgcgcac attcagcggt gacttaatca gcgaggccag cggcacgctc acttcctccg
     6841 tctgcgccag cagggccgac aggactgttt cctgcgtttt tgcaggttta cggctggatt
     6901 tacgctccgt ttttgcctta gactctgtaa ctgacatagt tagtacctcc caaaacggta
     6961 ataatgattg tctgccggac gcgttcccgt ccggcacctc tcttacttaa taatcccgaa
     7021 atcacggatg acatggttca tccagtaccc ggcaccgtcc tcaaaattcc aggtacgcca
     7081 gaccattgca ccgttattac ggacaaccag gcgaaactgt tcgccgtggt cattctcgat
     7141 aaagacgttc cggtactgtg cggcaacggc ttcggcatgt ttgcgggtaa aaggaccgtc
     7201 aggcagtccc tgaagtattc tgaaatattc gctcatcctc atctctccgg tgatgccccg
     7261 cacaggacgg ggcggttgtc acagtcagtc gttcagaacg ggatatcgtc tgaaaacccg
     7321 taatcgtcac cctccggcgg ttgcggctga ggctgaccgc tgaactgttg cccctcttcc
     7381 ggctgagtct gagcacctgc ggcacgtacc agcatctgca tggtgcccgt ggtcttaaca
     7441 agaatttcgg tgacgtaacg ggtgataccg ttatcctccc agctacgggt gcgaagctga
     7501 ccttcgatgt agacctgcgc gcccttgcgc agatattcac ctgccacttc cgcgagcttg
     7561 ccgaacagca ccacgcgatg ccattctgtc tgctcccgca tctcccccgt ctgtttgtca
     7621 cgccagcttt ctgacgtggc cacctgcagg tttgccactg cgcccccgtt ggggatgtaa
     7681 cggacttccg gatcttttcc caggcgtcct acgaggatga ccttgttaat gccacgaact
     7741 gccatgatgt gtctccttct gttgatgttt tcagccccca tcatcttttg agtttcggga
     7801 gccgcggttt tgacgttttg cggggacctg ccttttgttc cctccggtat tcaccatcac
     7861 ctttttgaga accttcgccg caaggggcat cactgccacg gccggaaacg actgtccggg
     7921 ggcagaccgc aaaacttttt gcctgctttt gggcaaaaag ttttgcggga tgtgcatttc
     7981 acccctggac tggcggagac ggttgtggct gtctttgccc ccggcgacag gggcttaaac
     8041 aggtgaatgg caccggacac aacaacgggc aggcacccgc cgcaacgacg tgaagcgcgg
     8101 tttgcgaaac ggcgttgccc cttacatccc ggcggccttt tgccgggatg ccgggagcgc
     8161 aggccacggc gcgacagagg cggcgcgacc ggaacggtcg ctgcaagccc gggtttcgcg
     8221 tcagggatgg aagcccgcca gggcggagac agctccgctg gctccgttca cgacagcccg
     8281 ccccgccagg gggacgcccg gaaaaggcat aagcaggaga acaacaaaac agacagtaat
     8341 cagagcagca gaaaaaagaa gaacagtagc agtagcagta gcagtagcag tagcagtagc
     8401 agtagcagta gcagtagcag tagcatgaac aacaacatca tcagtaacag acaaatgaca
     8461 ggtctgaaaa gacagaagcc cacagcagaa tgtccgcgta atgtaagggg cggcgcgtga
     8521 gcgcggcccc tttcagcgcc accggcgcgc ggtcttttcc cggcgcgggc agacgggcat
     8581 ttatcctccc gcctgcccgg agtcatcagg cacgcagacg aataatcatc gtcggaacat
     8641 cggtataagc aaacgtgccg cgtggcagct cctcgcggac gtcagaaaac ggtaacagct
     8701 tctcctgctg gcgcggcccg ttcagacaga cggcgaccag cacgccaccc ggacgcaaca
     8761 gggaaaaggc ccgcaggata tgccggatat cctgcccgtg gctgaacggc ggattaatga
     8821 taacccggct gtaatactgc accggctgcc attccatgaa gtcgccacac tgaaccctga
     8881 caccgttaaa attttcccgc agataccgga ccagcccgct gttgatttcc accgcatcac
     8941 acatggcctc cggtgcggta tcccgaatcg cccgcagtat ggccccggtg ccggcagagg
     9001 gttccagaat gtgatcacgg ttgctgatct ccgccagcgt caccaggcga tcacagaccg
     9061 gggcaggagt cacaaaaagc tgagattcag cccggatttc agcaaccggc gcggcgttat
     9121 ccagccttgc gcgcatcagc cggatacgtt cttttaactg actgtgcatg tcattatctc
     9181 cgtcatcccc cggggatttc ccggggggat ataccttatt tctgtgggat ttccgtaatt
     9241 ttcatgtcgg tgatatacac attcaccaga cggaaattat tgtccattgt gcggcggtag
     9301 cggtatgccc cgtggtcttc tgcttctgcc acactgcgca cggccttaca gtcccggggc
     9361 agggcggccc actgtgcctt tgtcatttcc cggaaacctt cccccggata gttgactacc
     9421 ggcggacgct tcgcggcctg gctggcgacg gcagcctctt ctgccgatgg tgctttgtag
     9481 tccgtgatgc gatcgggcgt cactttcatc gtgccgctgt acccgagaaa actgtaattc
     9541 ggcgttgtga ctgaactcac cgccccgttg cttttgttca cgcggatgat ggtcagccac
     9601 tcgccccggc tgaaaacctg tccgcccggc tcaaaatcct gtgtccgggt aaccacgccg
     9661 ccgctttcgt ccagcatggc acgctcatag ttcaggcggt tctgatagtg gttaacccag
     9721 cgttgctgat gctgaatctg ccgttcatga cagcggatcg caatttcgcg ggcctgctcc
     9781 gtggtgatga tgtcatcatc cagggccgac cataacgacc ggctcccttc atactggctt
     9841 ttttctgccg ggcgcggata cgtatccagc gggaaacagg cgctgatatg gtcatgactg
     9901 ctgatgagtt ttgccatatt cagatccagc gattcagccc gccacatcgt cagatatttc
     9961 tgcgactgcg cgatggtctt ttcagccttg cgcaaatcag cttcgatttt tttgatacga
    10021 cgccagcgaa cgtccggacg ttctttatac ttcgcgtgaa gcagtgctga ccgcccccgt
    10081 tcttcccagt attccgcacg ttcaaagagc atcacggcac gtttcatgcc gttttcaata
    10141 cgctgcgcat cacgacgggc gcggcgttcg ctgtgatgcc ccacaagaat gggctgaccg
    10201 ggcgggatca tcgcggccag tctttccact tcatcaagtg cctgtgcgga ttcactggcc
    10261 cgctttccgc tgtatccggt aaaccgctcc gcccgtgctt cctgacgttc agcgagcgtg
    10321 ctgtcttcat cctcaatctc tccggcaagt gagaggagca cgtcttcccg gcccggtgtc
    10381 caggccggcg cgacaaacag cgcctgtttt ggtgcccagc gaaaaccggc atcatgcact
    10441 tttttatacg tctccgggtc aagacgtgac acggcataca ggcgcagttt gttgtcatcc
    10501 ggtgagtagg tcgcgcggta catctgcaca gggctggcgg cttccggggc ggtcatggtg
    10561 attgctgctg atgtcataaa atgttctcct tccgtctgtt gccgcccctc gcggggcggc
    10621 tcacctcatc agttaaaagc gcgtttcagt tcgagtgtgc ggagcacctg cccgaaagcc
    10681 gtgtttttca tggcggggat gtgtacacca cgcccccggt gaaaactccg ggctatgtgt
    10741 cgcccgaagg ctttcagcac ggggtcaatt tccggagcgg ggatgtcttc gcgtctgaaa
    10801 cggggatgac ggcggggctt tacagccgct gcggaaggtg aaacaatgct tacagtcata
    10861 aatggattcc ttatgacaaa ttggttaaca atgccgtcaa aattggtcgc acaattctta
    10921 cggcgtggat taagcaggtc cctgacctgc ttttttcata tctgcatctg acgctttccg
    10981 gtgttttcct ccttctgttc ctgcgttctc attacctgtc agccggatga ctgacagaac
    11041 acataaatgc gtattgccgg agcctgaaat aaatcctgat ttttaaagaa catcacccgt
    11101 tgagtttcgg gagccgcggt tttgacgttt cgcgggaacc tgctgctgta ttccctgtgg
    11161 tatgaacttc acccgtttga gaaccttcgc cgcaaggggc atcactgcca cggcctgaca
    11221 cacctgtcct gggcagaacg caaaattttt taccgggttc tgggtaaaaa attttgtggg
    11281 ctgttcattt accctggaca gtcagtgcgg caggttgtgg ctgtatcagc tcccggcgac
    11341 agggactcat acgggtgtgt ggcacccggc aggcaatgag cagggacccg ccgcaacgac
    11401 gtgaagcgcg gtttgcgaaa cggcgttgcc ccttacatcc cggcggcctt ttgccgggat
    11461 gccgggagcg caggccacgg cgcgacagag gctgcgcgac cggaacggtc gctgcaagcc
    11521 agggtttcgc gtcagggatg gaagcccgcc agggcggaga cagcttcgct ggctccgttc
    11581 acgacagccc gccccgccag ggggacgccc ggaaaagcca taagcaggag aggaacggaa
    11641 agaagaggag gaagaggaag aggaaaaaga agaagaacag taacaggcac ggtaaccaca
    11701 gaaaaataac atcagaaaaa aacattccgg acacatcaca aacaggaagg gtaacggacg
    11761 acgcgtcagc gtggcccgct tcccttccgg caggcgtcag cctgccaggg gttttcgcgc
    11821 gggctgacag gcggcctcca ccgccagccc tgtatcacct gtttctgctg ccctgtaaga
    11881 tgtagtgacc gcccaggaaa acagcctgct gattcgccac aaaatcgcag atttcatcaa
    11941 caatctgcgc ggtggtgagc ggtttctgcc ccatttcacg acgtcgcgca ttaatcacgg
    12001 ctttgtaatt gcgcagaatt tcgcgggttt ctgtgctgat attcatgaca tgtgctcccc
    12061 gtccagcgcc tgtaacacaa cgtccattgc ttcgcgttcc tccctgacga acgcatcacc
    12121 gtcgcccccg ttctcactcc cgacggcata caggcgcttc atttcactgc ctgccagcgc
    12181 catcactttt tccagcatca ccgccggaac tgtcacctga cggctggcac gcttttcttc
    12241 catcaactca cgaacaacgt cgtgaacaat gctgacatcc gcaccatatt caaaggcatc
    12301 gtccagccgc tgcatgacgg tttccagctc atcgtctgac aggttccagt cctgggctat
    12361 gtggcgcaca tccccacggg tcagcaccac ggcaaaaacg cattcactgt ccggcacgtc
    12421 tgtatccagt acatcgcgga tgatttcttt aacagtacag tacatgttct cgtctccttt
    12481 acaggggcgg cattgccgcc ccgtccgtta atcagtccag tgccgcgtaa atcgcgttgc
    12541 attcagggtg aaattcgatg tgacgccaca actgcgcatc gcgcatccgg taaagctggg
    12601 tcagtcctgc atcaccgctg tcgtggtaca gccacaactg gcggttaatc acaagggaag
    12661 tcagaatgat gccgcaggca tcagcactca cggtacggtc aaaccagttt gcaccgttca
    12721 ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc accgtccggc aggcggatga
    12781 agtgccagaa gcctccctga tattccggca tcattttttg cgccagcgcg tacacatacg
    12841 cctcggcata cagaaaatcg ttgccaaaca ggtaaggcag aaaggagaga cggtcgcact
    12901 cttcaacatt cagagtgaca ggtttcgcat attgcatgat gttttcctcc gttgttcaca
    12961 tattccgcgg caccgcccgc acacgacgct gaggccacat aactgaaaaa ccacaaggcc
    13021 atgccctgca tggccgggta aagcggagcc agcggcgact tcctttgtgc tctgtccggg
    13081 gaccggcagg aaaaaggcgg ggatttaccc cgccaacgtc atcatttccg gatttttgcc
    13141 agggcatccg ccatgctgcg gatttcgttc cgggcttccc ggaccacctc ccgcagaaca
    13201 ccacggtcaa accaccggcg gggcggttca tccggtatac gcagcacatc gcaggccagc
    13261 ccactggaca ggatatgttc aatacaatcg ctgaccagat acatttccac cgccgaaaaa
    13321 cgtgcgcccc ggttcaggat ggcatcgagc accacgggca ggttttcgtc gtcctcatcg
    13381 agccagcagg ggtgatcgct ttccttcacg accaggcgaa agcggcgcgt ctggcgggtc
    13441 agtaacgtct gaccggtggt ggtcagcgcc gcccggatac gccgcgtatc ttctgccacc
    13501 cggcaactga cactgcgtac cgcatcgcgc accgccgcaa gtacagccct gatttcgtct
    13561 tcggtcagcc attcaccggc atacggaaaa gacacctgcg ttccgtctgc cggaagcacc
    13621 ggcagcgact gccactgact gccggacaga acaagttccg ctccggttcc ggtcagtttc
    13681 tgaatggcgg aggccaggtg cggcggcagt acgccgccgt ggcggttaac gagtaacgtc
    13741 gccagccggg gcgcatccag cagaacataa atgctgatcc agcccggata cttcgggtta
    13801 cgctggtcga catccgtctc ttccggccag ccctgcgcca gcagcaggtt acgggcgtga
    13861 cgacaaatca gtgcgttcag cgttgtgttt tcgttcatga ttaatcgttc tccctgtatc
    13921 gccgcccgca ggcggcgata acatcagccg ttttcgtgtg tgttgttttt cagcaggatg
    13981 cgggcctgat taaccaccgc ctgacggcgg gtaacgcatt ccgggacggc gagaccgtaa
    14041 tcacgggcgg cccattcacg tttccacagt gcctgttcgg cggtctgaat cagggacgcc
    14101 agcagttcag ccgggacggt cacctggcgg ttttcgctca cgttgctgat aatctccatc
    14161 gccgcggcag aagaaatacc ggactcaatt cgctggtctt ccgggatgtc ctccaggcgc
    14221 gccaggacgg tcctgatttc aggatcggac agggaaatat ccattccgtc agcgagggcc
    14281 tgaatctctt ccggggacca gataaccagc atcagcggtg tatatcccgg atactttgct
    14341 gccagctccc ggcatagcgt ttcgcatgtt ccgtacattc tcgtctcctt ctcaatcttt
    14401 aaagcacgca tccagttcca gaatttcggt atacgcctcc atgaacaggc gattggcgcg
    14461 atacagcgtc tggcgtgcca ggcgttcaac aagcacatcg gaatcaaagc gacagcgatg
    14521 ctccatatcg ttcagcctgg cccgaaggtc acacatggtg ttggccacct ccgccacgtc
    14581 ggcgcgaagt ttttcatgtc cggcatagtt catttacgca gcctccggca taaaccagtc
    14641 atcatttgcg gccccctgct gcatggcccg ctgtacggca gccagacgtt gctgtccggc
    14701 acggtgatac tgttcaagca gctcgatacc gatataccgg cgtccggact gaagggcggc
    14761 cacacaggtt gagccgctgc ctgcaaacgg gtccagcaca atcgcgttcg ggtgtgtgaa
    14821 gctctcaatc agtggttgca ggctggtaac aggcttttcc gtcgggtgat gacgattgcc
    14881 tgaatatttc cagcccagca cgtcgggcaa tggcttctga ggcagcgccg gacgcccttt
    14941 tgccaggatg taagcgcatt cgtggcgata gcccacatat gcggccttcg aagtgtaggt
    15001 tttggtaaac accaggtggc caaccacgct gaatcccgca tttttccagg cggccataaa
    15061 gcgatcgacg cggttccagc catagaagct caccatcagc gcatcttttt tcagcacgcg
    15121 gtacatttca ttacaggccg gttgcagcca ttcatcggtt ttatcgccgg cgatggtacg
    15181 cccctgacgg tcacggaaac cgacgagata tggcgggtcg gtgaggatga aatcgacacc
    15241 attgcccgga atcgtggcca taacacgcac gcagtcaccc tggataaaac gggacataac
    15301 agcactcctt ctcttacaga gggcaccggg agggcgcccc tgtggcgccc gttccccgcg
    15361 gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc gaccgtggaa tatcgcagcc
    15421 gtcgtctttt tgacggcgag aatatgccgc gaaagcggct tgaagggcgt tcatcagaaa
    15481 agggcgacgg gctggcgtac actgtcaccg atgccagccg ggagcggtaa aagcgcccgt
    15541 cccgtgcccg gccggacgcg ggacactgac cacaggcagc gttcctgccg cgccgcgtca
    15601 gccggcgcag taaaaagagg agcccggcga catcctttac cggaaaagaa acgggcggtt
    15661 gcccgcccgt ctccgtcata cttcatccac cgtaatgcgc caccttgtcg actatccact
    15721 ccggcgcggt gacttccggc agctcatcat catctgtcgg ggaagaccat tccagttccc
    15781 cccacagcac atcaacaagc tctccacgct catatcgctg ccagccacag aaattgcagc
    15841 cctgctcggc gtaccagtgc tccagcatgc aactgaaacg gcggcttaat actgcaataa
    15901 cgtcgctttc cggctgacac cacggcgtgt cgaaatcaac ctgaatgaag ttgtctccct
    15961 gactgctgat attcacctca tacccacaag gccacttcac gccatactgt tccgtatacc
    16021 agtgatatgc agaaggaaca ccgttcagaa caccaccgtt aaagccattc acttcaacat
    16081 ccaggcgagt ggacagaacc gtcagcaggt caaacggcat ggttttttcc ggcaggacgt
    16141 tgtcacacag acggttccac cacacagata catcctcgtt actccagatg tcgcaccagt
    16201 cacctctttt tggggtgaaa agagcggtaa tgctttccct ggcatcatcc ggtaattctt
    16261 cccagcgacg ccggccagta ccgctctgca gccagagttc atgcagcatc aggcagtttt
    16321 gctcatccag aagtacaccg tcctgcaggt gtgtcagcca gcgggtgaac gcgatattct
    16381 ccggcgatac aacgccacgc ccggcagccg tcagtccggg gcatggttca aaccgcacat
    16441 cttcagtggt ctgcagaagt ccggcacttc cggccagaaa cagctgaata ccttcatttg
    16501 tggcgcggcg ataaagcggt gtgactgcac cgctggccag tcgtttaatc tcagcgatct
    16561 gtgccggttc accagaaaaa tacatacgat tcgagcacca gtttggcatg ggaattctcc
    16621 tctcacatgc ggcctccatc aggccgtgcc gcacctgtaa ccgggcgacc gcatacgaat
    16681 gcaggtgcgg ccccgaagcc gcagcgcgtg gaggaggaac gacgaaacgt gaccgggtga
    16741 ggaggccggc gacagcgaca cacttgcatc ggatgcagcc cggttaacgt gccggcacgg
    16801 cctgggtaac caggtatttt gtccacataa ccgtgcgcaa aatgttgtgg ataagcagga
    16861 cacagcagca atccacagca ggcatacaac cgcacaccga ggttactccg ttctacaggt
    16921 tacgacgaca tgtcaatact tgcccttgac aggcattgat agaatcgtag tctcacgctg
    16981 atagtctgat cgacaataca agtgggaccg tggtcccaga ccgataatca gaccgacaac
    17041 acgagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg ggaccgtggt
    17101 cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc tgattatcag
    17161 accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt tatgctttct
    17221 ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac gtggtcgcat
    17281 cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat acactcagtt
    17341 ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa tactgtcgct
    17401 ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga cgttttaagc
    17461 acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc ttttttctgc
    17521 tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa ggctttttga
    17581 agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc aacaactgat
    17641 ttaggcaatt tggctgtgtt gatacagcgg gtaataatct tacgtgaaat attttccgca
    17701 tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc ataacgctga
    17761 ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa tgcagccatc
    17821 tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag tgcagcagct
    17881 ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg accgaacgcc
    17941 ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc gtcctcagta
    18001 agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc aacactatca
    18061 ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa agtaatggca
    18121 ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg ggcagctggt
    18181 gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg aataacaggc
    18241 gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa atttcattgc
    18301 agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag cgttgatcaa
    18361 tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca tccgtttcac
    18421 gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc tccatccacg
    18481 gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga
    18541 accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg
    18601 aggtgtagtc aaacaactca gcaggagtgg gaacaatcag cacatcagca gcacatacga
    18661 cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca tcatagtcat
    18721 gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc agtttacctt
    18781 catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag gaaggaataa
    18841 tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc ttttccccaa
    18901 gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc catccgtgat
    18961 acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt agccccttca
    19021 gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg
    19081 cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca
    19141 catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca acccgtcctc
    19201 gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg atagcctgag
    19261 aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt attttcctcg
    19321 cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag
    19381 cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt taatcattgc
    19441 tttgcatttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc
    19501 atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag ataagaagag
    19561 cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac cgagcatagc
    19621 gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt acgctcagcg
    19681 caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc ggatagccaa
    19741 ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta acccccgata
    19801 tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg ccctcaaatt
    19861 tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa
    19921 cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga accaccctca
    19981 aatctgtgac agataaccct cagactatcc tgtcgtcatg ga
//
LOCUS       DAZGXS010000080        19972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGXS010000080 DAZGXS010000000
VERSION     DAZGXS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            58..582
                     /gene="lpfA1"
                     /locus_tag="ACHKG5_003057"
     CDS             58..582
                     /gene="lpfA1"
                     /locus_tag="ACHKG5_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFP0869115.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            647..1333
                     /gene="lpfB"
                     /locus_tag="ACHKG5_003058"
     CDS             647..1333
                     /gene="lpfB"
                     /locus_tag="ACHKG5_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP0869116.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1362..3935
                     /gene="lpfC"
                     /locus_tag="ACHKG5_003059"
                     /pseudo
     CDS             1362..3935
                     /gene="lpfC"
                     /locus_tag="ACHKG5_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3950..5005
                     /locus_tag="ACHKG5_003060"
     CDS             3950..5005
                     /locus_tag="ACHKG5_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0869117.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5010..5540
                     /gene="lpfE"
                     /locus_tag="ACHKG5_003061"
     CDS             5010..5540
                     /gene="lpfE"
                     /locus_tag="ACHKG5_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP0869118.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5795..7486
                     /gene="eptB"
                     /locus_tag="ACHKG5_003062"
     CDS             5795..7486
                     /gene="eptB"
                     /locus_tag="ACHKG5_003062"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0869119.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7578..7654
                     /locus_tag="ACHKG5_003063"
     tRNA            7578..7654
                     /locus_tag="ACHKG5_003063"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7612..7614,aa:Pro,seq:cgg)
     gene            8399..10006
                     /gene="dppA"
                     /locus_tag="ACHKG5_003064"
     CDS             8399..10006
                     /gene="dppA"
                     /locus_tag="ACHKG5_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0869120.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10157..11176
                     /gene="dppB"
                     /locus_tag="ACHKG5_003065"
     CDS             10157..11176
                     /gene="dppB"
                     /locus_tag="ACHKG5_003065"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0869121.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11186..12088
                     /gene="dppC"
                     /locus_tag="ACHKG5_003066"
     CDS             11186..12088
                     /gene="dppC"
                     /locus_tag="ACHKG5_003066"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0869122.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12099..13082
                     /gene="dppD"
                     /locus_tag="ACHKG5_003067"
     CDS             12099..13082
                     /gene="dppD"
                     /locus_tag="ACHKG5_003067"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0869123.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13079..14083
                     /gene="dppF"
                     /locus_tag="ACHKG5_003068"
     CDS             13079..14083
                     /gene="dppF"
                     /locus_tag="ACHKG5_003068"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0869124.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14113..15384)
                     /locus_tag="ACHKG5_003069"
     CDS             complement(14113..15384)
                     /locus_tag="ACHKG5_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0869125.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15860..15967
                     /locus_tag="ACHKG5_003070"
     CDS             15860..15967
                     /locus_tag="ACHKG5_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0869126.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16054..17733)
                     /gene="bcsG"
                     /locus_tag="ACHKG5_003071"
     CDS             complement(16054..17733)
                     /gene="bcsG"
                     /locus_tag="ACHKG5_003071"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0869127.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17730..17921)
                     /gene="bcsF"
                     /locus_tag="ACHKG5_003072"
     CDS             complement(17730..17921)
                     /gene="bcsF"
                     /locus_tag="ACHKG5_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0869128.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17918..19489)
                     /gene="bcsE"
                     /locus_tag="ACHKG5_003073"
     CDS             complement(17918..19489)
                     /gene="bcsE"
                     /locus_tag="ACHKG5_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0869129.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19762..19950
                     /gene="bcsR"
                     /locus_tag="ACHKG5_003074"
     CDS             19762..19950
                     /gene="bcsR"
                     /locus_tag="ACHKG5_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0869130.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         4999 a         4859 c         5267 g         4847 t
ORIGIN      
        1 tcgattttaa tacatcaaga ttttcttttt aatgtaattt tttaaatgga gtttttcatg
       61 aaaaaggtcg tttttgcctt aaccgcactg gcgttaactt ctggcactgt attcgcagca
      121 gagtctggtg acggcacggt taaatttaca ggcgagatcg tggattcacc ttgcgtactg
      181 tccgttgact ctcagaacca ggaagttgtg ttgggtcagg tacaaaaatc tgtatttgct
      241 gcggttggtg acaaatcccc ggcaaaacct ttcgaaatca aactggaaga ttgcgatacc
      301 accacgatga aaaaagccaa tgtgagcttt agtggtgttg gtgatgcaga taaatctgat
      361 ctgatttctg ttagcactga agcgggcgcg gccaaaggtg ttggtatcgg tatttatgat
      421 aacagcaata ccctggtcgc tcttaacggt ggtaaagcaa gtgttgatct gagtaaaggc
      481 cagacggttc tctactttac cgctaactac gtttctactc tggcaacggt aacgaccggt
      541 tacggcaatg cgcaggtcga tttcaacctg tcttacgagt aatcgtccgt tttacaaatg
      601 gcggcgtaat tgccgccatt agtcgtcaaa gttcatggat cgaatgatga aaagtaaatt
      661 tgttgcgttg gctctctctc tgttcctttc acagtcagtt ctggcgggtg gcgtcggact
      721 tagctctact cgcgtaattt atgatggcag taagaaagaa gcctcgctga ccgtacagaa
      781 taaaagtaag acggaagagt ttcttatcca atcctgggtg gatgatgccg cgggcagcaa
      841 aaaaacaccg tttattatta cgcccccact attcaagctt gatccagaaa agaacaatat
      901 tttgcgtatt gtgaatatta cgcccggcct gcctcaggat cgcgaatcgg tctactgggt
      961 taatgtgaaa gccattccat ctaaaagtga cgacagcgaa aacaaaaatg ttttgcagat
     1021 tgccgttcgc acccgaatca aactttttta tcgcccggca ggtttaaagg gtgatgtaaa
     1081 aaccgcgcca aatgaactgc gatttacgcg taacggtaat cagctccgcg ttgataatcc
     1141 gacagttttt aatattacct ttaatcaatt ttttgcgaat gacaaagaaa ttgaaaaagc
     1201 aggcatggta cccgctaaag gggcattaaa tatcacatta cccgccgggg taggtagcgt
     1261 ttctaaaatt aaatataaca ccatcaatga ctttggatcg tcagcggaaa tgatcactaa
     1321 aaatgttgat tgattaatat cttttcagga gaaatagctc aatgtcacgg aagaccgttt
     1381 cacgtacttt ttcttccttt tctatctctg tggtggcggt tgctgttgcc agcacgtttt
     1441 ctgcacatgc cggaaagttt aaccccaaat tcctggagga cgttcagggt gtgggccagc
     1501 atgtcgattt aacgatgttc gaaaaaggcc aggaacagca gttacccggc atctatcgtg
     1561 tctctgttta cgtcaatgaa cagagaatgg aaacccgcac actggaattt aaagaggcca
     1621 cagaagcaca gcgcaaagcg atgggggaat cccttgtgcc ttgccttagc cggacgcaac
     1681 tggcagagat gggcgtgcgc gttgaaagct ttccggccct caatctggtg tctgccgaag
     1741 cctgcgtacc gtttgacgaa atcattcccc tggcctcaag ccatttcgac ttcagtgaac
     1801 aaaagctggt attaagtttc cctcaggcgg cgatgcacca ggtggcgcgc ggcacggtgc
     1861 cggaatcttt atgggacgaa ggtatccctg cgttgctgct ggattacagt tttagcggga
     1921 gcaacagtga atatgactcg acggggtctt ccagcagtta tgtcgatgat aatggcactg
     1981 ttcatcatga tgatggtaaa gatacgctaa agagcgacag ctactacctc aacttacgca
     2041 gcgggctgaa ccttggcgct tggcggttac ggaattacag tacctggagc cattccggtg
     2101 gcaaagcgca gtgggataat atcggtacgt ctttgagtag ggccatcatc cctttcaaag
     2161 cgcaattaac aatgggggat acggcgaccg cgggggacat ttttgacagc gttcagatgc
     2221 gtggcgcgat gttagcctct gatgaagaga tgctgcccga cagccagcga ggctttgcac
     2281 ccatcgttcg agggatcgcc aaaagcaacg ccgaagtcag tattgaacaa aacggctacg
     2341 ttatttatcg cacctatgtg cagcctggtg cctttgaaat aaacgatctt taccccactg
     2401 caaacagcgg tgatctcacg gtcattatta aagaagcgga tggcagtgaa caacgcttta
     2461 tttagccatt ctcatcggtg ccaattttcc agcgtgaagg ccatctcaaa tatagctttg
     2521 ccgcaggtga atatcaggcc gggaattatg acagcgcctc gccgcgtttc gggcagcttg
     2581 atctgatcta cggtttaccg tgggggatga cggcctacgg cggcgtatta atctctaata
     2641 attacaatgc atttacatta gggataggga aaaactttgg ttatatcggg gcgatttcca
     2701 ttgatgtgac gcaggctaaa agcgaactga ataacgatcg cgatagccag ggacaatctt
     2761 atcgtttctt atattccaag agcttcgaaa gcggcaccga tttccgcctt gcgggctatc
     2821 ggtactctac cagcggtttc tataccttcc aggaagccac cgatgtgcgc agtgacgctg
     2881 acagcgacta taaccgttat cacaagcgca gcgaaataca gggtaacctg acgcagcaat
     2941 taggggccta tggctctgtt tatttaaatt taacgcagca ggattactgg aacgacgcag
     3001 gtaaacagaa cacggtatcg gcgggttaca acggacgtat tggcaaggtc agttacagta
     3061 ttgcatatag ctggaataaa agccctgaat gggatgaaag cgatcgcttg tggtctttca
     3121 atatttccgt tccactaggc cgggcctgga gtaactatcg cgtcacgacc gaccaggatg
     3181 gtcgtaccaa tcaacaggtt ggggtcagcg gaacgctgct tgaggatcgc aacctgagct
     3241 acagtgtcca ggaaggctac gccagcaacg gtgtgggtaa cagcggtaac gctaacgttg
     3301 gctatcaggg tgggtccggt aatgtcaacg taggctatag ctacgggaaa gattaccggc
     3361 agctcaacta cagcgttcgc ggcggcgtga tagttcatag cgaaggcgtg acgctttccc
     3421 aaccgctagg cgaaaccatg acgctcatct ccgtacccgg tgcgcgcaat gcccgcgtgg
     3481 tgaataacgg cggcgttcag gttgactgga tgggtaacgc gatcgtgcct tatgccatgc
     3541 cgtatcgtga aaacgaaatc tcactgcgta gcgattcgtt gggtgacgat gttgacgttg
     3601 aaaatgcgtt ccagaaagtg gtgccaacgc gtggagcgat tgtcagagcg cgttttgata
     3661 cccgcgttgg ttaccgcgta ttaatgacgc tgcttcgttc cgcgggcagc ccggtgccct
     3721 ttggagcaac ggcaacgcta atcaccgata aacaaaacga ggtgagcagt atcgttggtg
     3781 aagaaggaca gctctatatt agcggaatgc cagaggaagg acgggtattg attaaatggg
     3841 gtaatgacgc gtcgcagcaa tgcgtggcgc cttataaatt atccctggaa ttaaaacagg
     3901 gcggaattat tcctgtttcg gccaattgcc agtaagcaag gagagcgaaa tgaaagcggc
     3961 aatagcatta tccttattag gatgcgtatt cggattttct ggcaaggcat ttgccggaga
     4021 tgcgtggggg ccatgtaccc ccgcggatgg cacgacttat cactataatg tcgatgtgga
     4081 tgttggtata cctgatgcgg caaaaaacgt cgcaggaacg gttttaccgg atgtccttaa
     4141 ctggtctaat ggacaaaacg tatcgctaat ttgtgaatgt cctgattctt ataagaatga
     4201 aaaagatact ctagtgcagg gggtgagtat gcttccccct tctggccgta cggttgacag
     4261 tatgaaatat tatactttga cggaggaact ggaggttgcg acgaacattc gcatcagtac
     4321 cagtgtgtat gggtttgttc ctttcaaaaa ccagcaggcg ctgcaaacaa ccggatgcaa
     4381 caaagtcatt actacgccct atatgggcgg cgcaggtcta ctttcctttg ctattactaa
     4441 accctttatc ggcgattccg tcatccctct gacgttaatt gccgaactgt atgcctcgaa
     4501 aacaaataaa gattacggaa caatacccat atcttccgta tctattcagg ggcgtgtcac
     4561 cgtaacccag gattgcgaaa ttaaacccgg cacggtgctg gatgtgccat ttggtgaatt
     4621 cccctcctcg gcgtttaaaa acaggcaagg gcaaatgcct gaaggtgcga cagagcaaga
     4681 aattaacctc agcttcgatt gcaacaatat ctctgatggt atcaaggttg cgttgcgcct
     4741 tgagggggca accaatgctg acgatccccg agcggtggat atgggcaacc ccgatattgg
     4801 cgtactggtg aaagattcca gcggcaaaat tttggtaccg aatgattcaa gcagcaccac
     4861 gttattaaat ttgagcagcc tggattctaa aactcaccgc aatgccgcga ttcgactgct
     4921 agctttaccg attagcacga cgggtaaagc gcctaaaggc gggacgtttg aaggcgtaac
     4981 gaccatatat cttgaaatgg agtgatgcca tgaagttcaa acgattgctg catagcggca
     5041 tcgccagttt gagtctggtt gcctgcgggg tgaatgcggc gacggatctt ggcccggcag
     5101 gggatattca tttctccatc actatcacca ctaaagcttg cgagatggaa aaaagcgatc
     5161 tcgaagtcga tatgggaaca atgacgctgc aaaaacctgc ggcagtcggt acggtgttga
     5221 gcaagaaaga tttcaccatt gaactcaaag agtgcgatgg gatatccaaa gcgaccgttg
     5281 agatggacag tcagtcggac agcgatgatg attccatgtt tgcccttgag gctggtggcg
     5341 caacgggtgt tgcgttgaag atagaggacg ataaaggaac gcagcaagtt cccaaaggct
     5401 ccagcggaac gccgattgaa tgggcgattg atggcgaaac cacgtcgctt cactaccagg
     5461 cgagttatgt ggtcgtcaac actcaggcca ctggtggcac agcgaatgcc cttgtcaatt
     5521 tttccatcac ctatgagtaa gttactgggc cagcatcctg gcccaaccta tctctgaata
     5581 gtcgttaatc cgttaaaatg gtctattccg gacttgcact tttgtaaatt tgtgcttcat
     5641 gcacactctt tccccacact ttttcccttt gctgtggtct acttattcgc gcgtgtagat
     5701 tttacttatc tgactacctc cgcacttttc ccctgctggg cctgaaaagc cgctaagcag
     5761 ggtgttatca cctgtttgtc cagggtttgt ttgcatgaga tacatcaaat cgattacaca
     5821 gcagaagctg agctttttgc ttgcaatcta tattggcctt tttatgaatg gcgcggtttt
     5881 ttaccgccgc ttcggcagct atgcgcacga ttttaccgtc tggaaaggca tttctgctgt
     5941 tgttgaactg gccgccaccg tactggtgac cttcttttta ctacgtcttc tttcgctgtt
     6001 tggccgccgc agctggcgta ttctggcatc gctggttgtg ctcttttccg caggtgccag
     6061 ctattacatg accttcctta atgtggtcat tggttatggc atcatcgctt ccgtcatgac
     6121 caccgatatc gacctgtcaa aagaagttgt tggtctgaac tttattctct ggttaatcgc
     6181 cgttagcgca ttgcctctta tccttatctg gaataaccgc tgtcgctaca ccttgctccg
     6241 acaactgcga accccagggc agcgtattcg cagcctggcg gtcgtcgtac tggcgggaat
     6301 tatggtttgg gcaccgattc gtttgctgga tatccagcag aagaaagtgg agagggcgac
     6361 cggcgttgat ttgccgagtt atggcggtgt cgtagcgaac tcttatctgc catcaaactg
     6421 gctttctgcg ttggggctgt acgcctgggc gcgggtcgat gaatcttccg ataataattc
     6481 attgcttaac ccggcgaaga aattcactta tcaggcaccg caaaatgtta atgatactta
     6541 tgtcgtgttt atcatcggtg aaaccacgcg ttgggaccat atgggcattt tcggctatga
     6601 gcgtaatacc acgccgaagc tggcccagga gaaaaatctg gcggcgttcc gtggttactc
     6661 ctgtgatacc gcaaccaaac tctctctgcg ttgcatgttt gtacgtcagg ggggcgcgga
     6721 agataatccg cagcgcacat taaaagaaca gaacattttc gcggtactga agcagttagg
     6781 attcagttct gacctctacg ctatgcagag cgaaatgtgg ttctacagca atacgatggc
     6841 ggacaatatt gcttaccgtg aacagattgg tgcggagcca cgtaaccgtg gcaagccggt
     6901 agatgatatg ttgctggtag acgaaatgca gcaatcgctg gggcgcaacc cggatggtaa
     6961 gcatctaatc attctgcata ccaaaggttc gcactttaac tacacccagc gttacccgcg
     7021 cagctttgcg cagtggaagc cggaatgtat tggtgttgat agcggatgta ccaaagcgca
     7081 gatgatcaac tcctatgaca actcggtgac ctatgtggat cactttatct ccagtgtgat
     7141 tgatcaggtt cgcgataaga aagcgattgt gttctacgca gctgaccacg gcgagtcaat
     7201 taatgaacgc gagcacctgc acggcacgcc gcgtgaactg gcaccgccgg agcagttccg
     7261 cgtaccgatg atggtctgga tgtcagataa atatctggaa aatccggtca atgcgcaggc
     7321 gtttgcgcag ctgaaaaaag cagccgacat gaaagtgcca cgccgtcacg tagagctgta
     7381 cgacaccatc atgggttgtc ttggctatac ttcaccggat ggtggaatta acgaaaacaa
     7441 caactggtgt cacatcccgc agacaaaaga ggcagcggct aactaagagg cctgctgact
     7501 ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt
     7561 aagatgcgcc ccgcattcgg tgattggcgc agcctggtag cgcacttcgt tcgggacgaa
     7621 ggggtcggag gttcgaatcc tctatcaccg accaaattcg aaaagcctgc tcaacgagca
     7681 ggcttttttg catctgcagc ccatgaggaa gagaacctcc gggggcaggg aggttcgact
     7741 cgacgtaggc ctgataagac gcgtcagcgt cgcatcaggc aacggcaccg cacttattgt
     7801 cggatgcggc gtgaacgcct tattcgacct acacgccaca catttccctg ccaaatttgc
     7861 gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct ttttttatat
     7921 ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg ctgtgcgttt
     7981 tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt tttcactaaa
     8041 tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct ggtcatgttg
     8101 gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat ctttgtttgt
     8161 ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac attctgttga
     8221 cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac tgatgttaat
     8281 tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa aaaaggtcag
     8341 gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga gcagaataat
     8401 gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg tggctatgac
     8461 cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat ctccggaagg
     8521 gtttaacccg cagctgttta cctccggtac cacctatgac gcctcttccg taccgcttta
     8581 taaccgccta gttgaattta aaatcggcac caccgaagtg attccgggcc tcgctgaaaa
     8641 gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag gtgtgaagtg
     8701 gcacgacaat aaagaattca aaccgacgcg cgaaatgaat gccgatgacg tggtgttctc
     8761 gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg gcagctacga
     8821 atacttcgaa ggcatgggct tgccggagct gatcagcgaa gtgaaaaagg tggacgacaa
     8881 caccgttcag tttgtgctga ctcgcccgga agcgccgttc ctcgctgacc tggcaatgga
     8941 cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg gtacaccgga
     9001 aaaactggac ctcaacccaa tcggcaccgg tccgttccag ttacagcagt atcaaaaaga
     9061 ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc agatcgatac
     9121 gctggttttc tctattaccc ctgacgcttc cgtgcgttac gctaaattgc agaaaaatga
     9181 atgccaggtg atgccgtacc cgaacccggc agatatcgcc cgcatgaagc aggataaatc
     9241 tatcaacctg atggaaatgc cggggctgaa cgtcggttat ctctcgtata acgtgcagaa
     9301 aaaaccactc gatgacgtga aagttcgcca ggctctgacc tacgcggtga acaaagacgc
     9361 gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga tcccgccaac
     9421 catgtggggc tataacgacg acgttcagga ctacacctac gatcctgaaa aagcgaaagc
     9481 cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg cgatgccggt
     9541 acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg cagactgggc
     9601 gaaagtcggc gtgcaggcca aaattgtcac ctacgaatgg ggtgagtacc tcaagcgtgc
     9661 gaaagatggc gagcaccaga cggtaatgat gggctggact ggcgataacg gggatccgga
     9721 taacttcttc gccactctgt tcagctgcgc cgcctctgaa caaggctcca actactcaaa
     9781 atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg acgaccataa
     9841 caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg ctccggcact
     9901 gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagttaaag gctatgtggt
     9961 tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa aagccatacc
    10021 cggtggtgct gtgcctctgc gtgcagcgcc atccggcagc aatacttcat tccctgcccg
    10081 acctgggcgg gaattgattt gtgagcaata cagacacgca gttccaggct gcgggtcact
    10141 acagagaatc cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac
    10201 gtttatcggt attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatcctgt
    10261 gatgatcatg gcgggcgaac gtgggatctc cccagagcgt cacgcgcagt tgctggctga
    10321 actcggctta gataaaccga tgtggcagca gtatctccat tacatttggg gcgttatgca
    10381 tggcgatcta ggcatttcaa tgaaaagccg aattccggta tgggaagagt tcgtgccgcg
    10441 ctttcaggcc acgctggaac ttggcgtctg cgcgatgatt tttgcgaccg cggtcggcat
    10501 tccggttggt gtgctggccg cggttaaacg cggttccatt ttcgatcaca cagcggttgg
    10561 cctggcgctg acaggttatt caatgcctat cttctggtgg ggcatgatgc tgatcatgct
    10621 ggtttcggtg cactggaacc tgacacccgt ctccggtcgc gtgagcgata tggtgttcct
    10681 cgatgattcc aatccgttaa ccggttttat gctgatcgac accgccatct ggggtgaaga
    10741 cggtaacttt atcgatgccg tcgcccatat gatcttgccc gccattgtgc tgggtactat
    10801 tccgctggcg gtcattgtgc gtatgacacg ctcctcgatg ctggaagtgc tgggtgaaga
    10861 ttacatccgc accgcgcgcg ccaaagggtt gacccgcatg cgggtgatta tcgtccatgc
    10921 gctgcgtaac gcgatgctgc cggtagtgac cgttatcggc ctccaggtgg gaacattgct
    10981 ggcgggggcg attctgaccg aaaccatctt ctcgtggccc ggtctggggc gctggttgat
    11041 tgacgcactg caacggcgcg attatccggt ggtgcagggc ggcgtattgc tggtggcgac
    11101 gatgattatc ctcgtcaacc tgctggtcga tctgctgtat ggcgtggtga acccgcgtat
    11161 tcgtcataag aagtaagggg tcatcatgtc acaggttact gaaaataaag tgattagcgc
    11221 gccggtaccg atgacccctt tacaggagtt ctggcactat tttaaacgca acaaaggcgc
    11281 ggtcgtcggg ctggtttacg tcgtcatcgt gctgtttatc gcgatctttg ccaactggat
    11341 tgctccctat aacccggcgg aacagttccg cgatgcactg ctcgccccgc cagcctggca
    11401 ggaaggcggc agcatggcgc acttgctggg taccgatgat gtaggccgtg atgtgctgtc
    11461 gcggctgatg tacggcgcgc gcttgtcgct gctggttggc tgtctggtgg tggtgttatc
    11521 gctgattatg ggcgttattc tcggcctgat cgccggttac ttcggcggcc tggtcgataa
    11581 catcattatg cgcgtggtcg atatcatgct ggcgctgcca agtctgctgt tggcactggt
    11641 gctggtggcg attttcggcc cgtcgattgg taacgccgcg ctggcgctga cctttgttgc
    11701 cttgccgcac tatgtgcgct taacccgcgc cgccgtgctg gtggaggtga atcgcgatta
    11761 cgtcaccgct tctcgcgtgg caggagccgg ggcgatgcgc cagatgttta ttaacatctt
    11821 cccgaactgc cttgcaccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct
    11881 cgatatggct gctcttggtt tcctcggcat gggggcgcag ccgccaacgc cggagtgggg
    11941 caccatgctc tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc
    12001 gggtctggcg atcctgctga cggtgctggc atttaacctg atgggtgacg gtctgcgtga
    12061 cgcgctcgat cccaaactga agcagtaaga ggttcgagat ggcgttatta aatgtagata
    12121 aattatcggt gcatttcggc gacgaaagcg caccgttccg cgccgtagac cgcattagct
    12181 acagcgtaaa acagggcgaa gtagtcggga ttgtgggtga gtctggctcc ggtaagtcgg
    12241 tcagttcgct ggcgattatg gggctgattg attatccggg ccgcgtgatg gcggaaaagc
    12301 tggagtttaa cggtcaggat ttgcagcgta tctcggaaaa agagcgccgc aacctggtgg
    12361 gtgccgaagt ggcgatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg
    12421 tgggtttcca gattatggaa gcgattaagg tgcatcaggg cggtaataag agcactcgtc
    12481 gtcagcgggc gattgacttg ttgaatcagg tcggtattcc cgatccggct tcgcgtctgg
    12541 atgtttaccc gcatcagctt tccggcggca tgagccagcg cgtgatgatt gcgatggcca
    12601 ttgcctgtcg gccaaaactg ctgattgccg atgaaccaac cactgcgctg gacgtgacca
    12661 ttcaggcgca aatcattgaa ctactgctgg agctacagca gaaagagaac atggcgctgg
    12721 tgttaattac ccatgacctg gcgctggtgg cggaagcggc acataaaatc atcgtgatgt
    12781 atgccggtca ggtggtggaa acgggcgatg cgcacgccat cttccatgcg ccgcgtcacc
    12841 cgtatactca ggcattgctg cgtgcgctgc cggaatttgc tcaggacaaa gaacgtctgg
    12901 catcgttgcc tggcgttgtt cccggcaagt acgaccgccc gaacggctgc ttgcttaacc
    12961 cgcgctgccc ctatgccact gacagatgtc gcgctgaaga accggcgctg aatatgctcg
    13021 ctgacgggcg tcagtccaaa tgccattacc cacttgatga tgccgggagg ccgacactat
    13081 gagtacgcaa gaggccacct cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca
    13141 ttatccggtg aagaaaggta tgttcgctcc ggaacgtctg gtgaaggcgc tggacggcgt
    13201 ttcgtttaac cttgaacgtg gcaaaacgct ggcggtagta ggtgaatctg gctgcggtaa
    13261 atcgaccctc ggtcggttgc tgacgatgat tgaaacgccc accggcggcg agctgtatta
    13321 ccaggggcag gatctgctta agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat
    13381 ccagattgtc ttccagaacc cttacggttc gctgaatccg cgtaaaaaaa tcgggcaaat
    13441 tcttgaagag ccgctgctga tcaacaccag cttaagcaaa gaacagcgtc gggaaaaagc
    13501 cctgtcgatg atggcgaaag tcggcctgaa aaccgagcac tatgaccgct atccgcatat
    13561 gttctccggc ggtcagcgtc agcgtatcgc cattgcccga ggtctgatgc tcgacccgga
    13621 cgtggtgatt gccgatgaac cggtctccgc gctggacgtc tcggtgcgtg cacaggtgct
    13681 caacctgatg atggatttgc agcaggaact ggggctttct tatgtcttca tctcccacga
    13741 cctgtcggtg gtggagcaca ttgctgatga agtgatggtg atgtacctgg gccgctgcgt
    13801 ggagaaggga acgaaagacc aaatcttcaa taacccgcgc catccgtaca ctcaggcgct
    13861 actttccgcg acgccgcgcc tgaatccgga cgatcgccgc gagcgcatca aactcaccgg
    13921 tgaactgcca agcccgctga atccaccgcc gggctgcgcg tttaacgccc gctgtcgtcg
    13981 gcgcttcggc ccctgcaccc agttgcagcc gcagctaaaa gactacggcg gtcaactggt
    14041 agcttgtttt gctgttgatc aggatgaaaa tccgcagcgt taactcacaa aaccggggcc
    14101 atgcgctccg gtttactcaa tcagcttcaa cagtggcgac aagcatagca aaatgccgta
    14161 aagcagaatc agccaggttt taaatccgcg aagtttttcc agttgtgcga ctttatagat
    14221 caggaaaaac ggaatgaggc acgacacaat gccatataac gggcttccca actgaaagaa
    14281 caccagcacc gatacacgaa acgaaaccca aatcgtcaac gtaatgacga taaaagcgca
    14341 gatcgccaga gtcagcacgc gtgagttaat tttcttggta tcaataattc ggcttaacag
    14401 attgagaata atgcctttaa tggcctcgtg gaaaccgaga taaatgccaa agaatgcggt
    14461 cagtacggca aagatattca gcaccgtaga ggtgatatga atgatatgcc cagggatcac
    14521 ctgcgcggcc agtgccagtg ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg
    14581 gctaattgag aaggtaaacg aaaaggcaaa aaacagtatc accgcgatga gcgtgatata
    14641 actaattcgg tgggtacgca gcgcgagccg ggttgccagt actttatccg cttcccgttt
    14701 acgataggca atattcattg ggttaagtac ctgaataaat actgcagaaa agaagcaaaa
    14761 tggaatggta agcaggacat cgcgaaaaaa gacggaggct tgcgggaagg cggttatatt
    14821 ggcgaaattc cagtgcggga tcatcgtaaa accgaacacg acaataatcc ctactttgac
    14881 caccaccatt ggcccagaaa tcttaaacag taatcgttca ccaccagacg caatcgctac
    14941 cagtacggcg aaaatagcga ctttataaag tagagattgt gaaagatcgg catcggttaa
    15001 accgaaggtt ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt agataaaaat
    15061 cccgtggata atcatcaaaa agtagataac cccgaggaaa attccccagt tcttccccag
    15121 gtaatgactg ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa
    15181 ataaatgtcc tgcactaccc aggtggcagg ataagcaatg atcgctgcgg taataaatac
    15241 ccaaatgccc ttcaagccaa tttgtactgg catcagcacg gttccggcac caatcgccat
    15301 gcctatgcat aataaaaccc agccgaagtc gtagcgtgta aacggcaatt tctgatcgtg
    15361 tttcggtagt gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa
    15421 tgaataaacc cagtttctct tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa
    15481 aatgaggcaa cagattcact ggggaggtcg cgaggaggcg caattctaca aaattgcgca
    15541 tttcactacc agtctggagt cagcccggat atgaactgca actattgcaa attaagaaat
    15601 taagctggaa ggcatcacgc aatgaggaag attgccgagt gctgacagta aaaagcaatc
    15661 gtaaaattag cggcaagccg gaagggttcc ggtgaggcgc aatgttgcgg gggcttaatc
    15721 cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac
    15781 aacaccacgg gggctaatct tgaacctaga ccactcaaga atagccgcga aacgttgtca
    15841 ttacaacaca ggcggctata tgacgctcgc agagctgggc atggccttct ggcatgattt
    15901 agcggctccg gtcattgctg gcattcttgc cagtatgatc gtgaactggc tgaacaagcg
    15961 gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccagac
    16021 cgcagggggg aaactctgcg gcctttttcg tgcttactgc gggtaaggca cccagtcgcc
    16081 gccgttcagg cgaacgtacg gtttatcctg gtattgaata actactgcat ttgagttctc
    16141 tgagaccggt gctgtttgtg gcaacccact ggtgagtttt ttccagtcaa cattgtcttc
    16201 ggtgaaaatc ttgccatcaa gaacgcgaac caccagatcg gagatagcca ggaagctgct
    16261 cggttggtcg atgacaatcg gtgccccctg atgtggtgcc ttcatgccga agaatttcac
    16321 cccaacgggg acgtcggtga tagacgggct agggatatca cgtaggccag atacctgcat
    16381 tctgtcgccc ttcagcgcgc cgccgtgttc cggcaccacg accaccatca ctttacgacc
    16441 cgatttctcc agttcagtaa agaaggcgtc cagttcatca aagaatttct gcgcccgcgc
    16501 tttgtaatcc gctgttttgc tgacgcccgg ataatggttg ccgtcatgca gtggaagcgt
    16561 gttgtagaac gtggcactac ggctgttttt atctttttcg gtaacgtcca gccagcggtt
    16621 aagcacggcg gtatcgtcat aaaccggcga accatcaaag cccagcaaaa taaccggcag
    16681 atttgtttga tccatcaatt cagtctgcat gccgccattt tcgcgaactt ctttcaaaaa
    16741 accgccgaac tggccgttat gtcccatcat caggtgctgg gtaaagccca gtttcgacag
    16801 gttatcaaac agatagcagt cgttatttgc cggttgatac agattagtgt gcgaagtctg
    16861 cccgcagctg gcgcgcagta aacggatcgc cgccgggcca ctgtaggagg tggcggagtt
    16921 aaagttcttg aactcaatat cgaaatgcga ccacagtgga tgcgacatca acccagcggc
    16981 ttctatatcc gaccaggaaa gcgaacagat gttaatcacc agtagttcaa atggctgagc
    17041 atcagcgggc agcgaagacg ggaaggtcga tttacgtttc gcctccgcgt tatagaaatt
    17101 attcagccag gcgttaaggt tcgccgttgt tggcggtgca gtttgtgcgg gcatatcacc
    17161 cactaccggt gcgccacccg tcgccgcaac ggttgccgct gcgttaccac ccgtcgttgt
    17221 tacagtggtc gtcggttgtc cggctggcca caaggagaaa cttggtcccg ccagggtaag
    17281 tacgttcagc catagcagta tggcaaccac aaaaacggta atgcgaatcc attgtgacag
    17341 gaataaccag gccactaata aaacaaaaat ggccccaatc atctgccagt taataaagcg
    17401 tgtgacaagg tcgattaaat aatcggtact gaaccccgcc acctgcgaac cctggctcat
    17461 tatgctttcc gggccaggca accaggtgtc atgccagaac aaagcaaagc cgatcggcag
    17521 ggcaatccag tggcgcaagc gatgcaggct gtagcgcgga atgggcatca gcagaaacgc
    17581 ggcaaacacc aaattgagga gcggatggaa gttaagatat cccgcccaca acaggccgaa
    17641 cttaaccaga aaatagaagt tccagccgga aaggccgcgc cagtattgcc agagggaaga
    17701 aggcatggcg gtattttgcg taaattgagt cattttttgg ttgccctggc tttttccgtg
    17761 cggcgtaacg tcccagccgg tttaacataa cgaggtttag caaagaacaa acgtaaggtg
    17821 tcgcgaatgc gtcgcaaaga gtgccgcgcc agatagccca gcgggaaaaa tatcagtgcg
    17881 caaacgacaa taatttcaat gatatcgctg atggtcatca tgatgagcgc tccacagcat
    17941 catctaacag tcgcatgggt tctggtattc gtcgccagtg gcgaccatcg tgctcggcat
    18001 tgatgaccgg tttagaactt tgcgttaaag gcagcggcat gccccattgt tctggggcaa
    18061 gcaggcgcat ctgcaccagc tcggcactga tttgatcatc ttcaaaccag accatacggt
    18121 ttgagaaaat gtcgccagta ggcaatggga aaatatgatt caacgcggta tccagatcgt
    18181 taatccgaca gaatgagaga aacagcacca gccgattacc gccaatggtc atgatatcgc
    18241 cggtacggtt agggcgacac agcgtcaggg cttgttcaac gcggataccc ggtaccggac
    18301 gtagggcaac cagaacgcct ttaccgtggg caggtaatag agggttattc atcatgttgt
    18361 tgacggcatt acagaacaca tcccacttct ggaaaccacg cagtttgagc ggctgggtca
    18421 ttgacagcaa ggtagtgata tcttccggca catagcgact aaacttctgc ccttgcacgc
    18481 tttcgatcat cgtcagacaa cgggagagtg gcgcattcca cggaataacc atatttgcac
    18541 cgcaggccaa taacaaacgt tcatcggtgg cgcgcaggct agcggtattt tcccgcacga
    18601 ggattttcat cgcactaccg cgctggcgac gcagggtatg aatgctgcgg gccagtggct
    18661 cgatttgcgc attttgctgt aaagaaaaga ccaccgtcgc cgcctgagcg gtacgggctt
    18721 cattgaacag gacttcgtta ttgttgaaca gttgccagtg ttccgatagc ggcggcgcac
    18781 cttccagtac agcaacatta ctcagaatgc gtttttcgtc gctgcgtggt tggatctccg
    18841 cctcttcgct ttgaactaat gtccagatac cattttgttg ctgaacgcta agctgctgac
    18901 gggcgctgac ccctttttcg ttgcaccaga aggcaatatc cagcaaatgt tggtcgccct
    18961 gaaaacgcaa actggcaaga ccaaaaagtg aacggtattc ctcaagcaac aatgaaaatt
    19021 gtttatcgtt attatttccg ggattaatta ccaaaagcga acaatggttt aacctgctcc
    19081 atttattcat tttatccaac catgagcgca gccgctcggc aggaatgttt tgccatgcgt
    19141 tatttgcgca aacaagaata aaaaggtaat tatggggatc aatagaacac tgcaaatcac
    19201 gggccaaata gtatagacct ttttcatgat ttagcattga aaataatttt attttttccg
    19261 gcccttgaga atcatctaat tgaaagattt tcgccggatc gctgtccatg ctaatgaccg
    19321 cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt cgccagactg atagcatctt
    19381 catggcgatc gacgttaaac caccagacgc cgcctgctgg catatgtcgc agctcatccc
    19441 ataatgatga gataccgata gagaatacag ggtccacaat gtccctcatg tcaccatttt
    19501 gtctgattat cagtttacta gcgaaagcta agaaataaac ctaccattga aattaaaact
    19561 tatcagattt agcatgtgaa tcaatttagc aggtcacaag acgtgtgatt ttagtctttt
    19621 cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa gtgagcaaat tcccgtctcg
    19681 ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt gtctcatgaa cggtacggtt
    19741 atttcatagg gatcaagcaa aatgaataac aatgaaccag atactctgcc tgatcccgcg
    19801 ataggctata tcttccagaa tgatattttg gcgttaaagc aggcattttc actgcctggt
    19861 attgattatg ccgatatttc ccaacgcgaa cagttggccg cggcattaaa acgctggccg
    19921 ttgctggcag agtttgcgca acaaaagtag gggattggtg aatggccgta ct
//
LOCUS       DAZGXS010000081        19799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGXS010000081 DAZGXS010000000
VERSION     DAZGXS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            285..785
                     /gene="tssB"
                     /locus_tag="ACHKG5_003075"
     CDS             285..785
                     /gene="tssB"
                     /locus_tag="ACHKG5_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0869131.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            820..1044
                     /locus_tag="ACHKG5_003076"
     CDS             820..1044
                     /locus_tag="ACHKG5_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869132.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1095..2486
                     /gene="tssC"
                     /locus_tag="ACHKG5_003077"
     CDS             1095..2486
                     /gene="tssC"
                     /locus_tag="ACHKG5_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0869133.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLDAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2577..2990
                     /gene="tssE"
                     /locus_tag="ACHKG5_003078"
     CDS             2577..2990
                     /gene="tssE"
                     /locus_tag="ACHKG5_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0869134.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2994..4757
                     /gene="tssF"
                     /locus_tag="ACHKG5_003079"
     CDS             2994..4757
                     /gene="tssF"
                     /locus_tag="ACHKG5_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0869135.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDNRRADYPLVPSRHYPQHYDVFSVNSVVSQVQDMFRKKDLG
                     RPVSTQAARQWPAFESFSHQMEYSRKREVVYWHHRTKTSLFHRGFDHTLAFIHADGSY
                     PSDESLLSNEVVSVSLTCTNRELPSQIRSGDITGTTGKNAAVASFRNITRPTQPLWPV
                     IDGSLHWSLLSAMNLNYLSLLDTDALKQVIANFDRHAIHHPQTARLSQQKLDAIERLE
                     TRPVDRLFTGIPVRGLASTLYLHPEPFVCEGEMYLLGTVLSHFLSLYASVNSFHMLTV
                     VNTESQETWKWTERIGQHPLI"
     gene            4721..5803
                     /gene="tssG"
                     /locus_tag="ACHKG5_003080"
     CDS             4721..5803
                     /gene="tssG"
                     /locus_tag="ACHKG5_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0869136.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5828..7108
                     /gene="tagH"
                     /locus_tag="ACHKG5_003081"
     CDS             5828..7108
                     /gene="tagH"
                     /locus_tag="ACHKG5_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0869137.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7105..7629
                     /gene="tssJ"
                     /locus_tag="ACHKG5_003082"
     CDS             7105..7629
                     /gene="tssJ"
                     /locus_tag="ACHKG5_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0869138.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7632..8963
                     /gene="tssK"
                     /locus_tag="ACHKG5_003083"
     CDS             7632..8963
                     /gene="tssK"
                     /locus_tag="ACHKG5_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0869139.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8968..9729
                     /gene="icmH"
                     /locus_tag="ACHKG5_003084"
     CDS             8968..9729
                     /gene="icmH"
                     /locus_tag="ACHKG5_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP0869140.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9738..12509
                     /gene="tssH"
                     /locus_tag="ACHKG5_003085"
                     /gene_synonym="clpV"
     CDS             9738..12509
                     /gene="tssH"
                     /locus_tag="ACHKG5_003085"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0869141.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12506..13249
                     /gene="vasI"
                     /locus_tag="ACHKG5_003086"
     CDS             12506..13249
                     /gene="vasI"
                     /locus_tag="ACHKG5_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0869142.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13254..14666
                     /gene="tssA"
                     /locus_tag="ACHKG5_003087"
     CDS             13254..14666
                     /gene="tssA"
                     /locus_tag="ACHKG5_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0869143.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14803..18237
                     /gene="tssM"
                     /locus_tag="ACHKG5_003088"
     CDS             14803..18237
                     /gene="tssM"
                     /locus_tag="ACHKG5_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP0869144.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQHLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18248..19657
                     /locus_tag="ACHKG5_003089"
     CDS             18248..19657
                     /locus_tag="ACHKG5_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP0869145.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19623..>19799
                     /gene="tssD"
                     /locus_tag="ACHKG5_003090"
     CDS             19623..>19799
                     /gene="tssD"
                     /locus_tag="ACHKG5_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP0869146.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHL"
BASE COUNT         4657 a         5447 c         5476 g         4219 t
ORIGIN      
        1 tataattcaa taagatacat aaaaaactct atatttaaat tattgccaca atacacctga
       61 cgcgaattgc acaggttcat aaaatctatt taacgcttgc agaatacccc tctttacgat
      121 cgataaaaag tccttttctt atttattttg aagcgtaaaa tgcggggtga tgaaggagtg
      181 gtgattttat tttcaccgcc atcaagacgg catttccgat attttttgcg gtacgaatat
      241 gccgtgttca tggcctgtac gagataacat gacggatcac ttttatgagc aaaaaatttg
      301 aaggcagcgt cgcgcctcgc gaacgtatta atatatcgta tgtcccaaaa acagatggcc
      361 agacggctga agtggaactt ccgcttaaca tgctcgttgt cggcgatacc ggaaatacgc
      421 aggaaacatc ctcactggat gaacgtcagg cagtttccgt taacaagcac aatttcggtg
      481 cagtgatggc tgaagcagcc attggcctca attttaccgt ccccgccacg ctgaagggaa
      541 gcacaacgga cgatgaaatg aatgtggcgc tgaatatcaa gtcactggat gatttttcac
      601 cagatagcgt ggcccggcag gttccggaag tgaataaatt gctggaactg cgtgaagcac
      661 tgactgcact gaaggggccg atgggtaacc ttccggcatt ccgtacccag ctccaggcat
      721 tgctggaaaa tgaagagtcc cgcgagcagt tgctcaaaga aatcggccag gtgagcaata
      781 aataaccacc actatttcat taaacagggt tatccaggca tgacggcaca caaaaatatc
      841 tccgggacat accacctctc tgttgcagac attctgcagg tggtttacca ggtatgtttc
      901 agcccgtccg ttgaaatcaa ccaggatggt gtggctgcgc tgattacaac actggatcgc
      961 cgtatcagtg atctattgga tgagattatc catttttgtg agtttcagca gagcgcgtcg
     1021 cactggcagc gcgtacttca ctgacggcga aaagccggtt tcagaaacac agctaaaaca
     1081 gaaggatttt cagtatgtct ttacaggaag aggaactcgt ttccagtcat gccgggcagc
     1141 ctgagcagga atcgtcgctg cttgatcaaa tcatggcaca gacccgtatt cagcccggtt
     1201 cggaaggtta tgacgttgcc cgccagggtg tgacagcctt tattgccagc atcctgcaat
     1261 ccactgcatc tgccgaaccg gtcaataaac tggccgtgga cagcatgatt gcagatattg
     1321 atgaacgcat cagccgccag atggatgtca tcatccatgc gcctgccttt cagcaggtcg
     1381 agtcgttctg gcgctcactg aaaaccatgg tggatcgcgt tgatttccgt gaaaacatca
     1441 aggtcaacgt gcttcatgtc accaagcagg aacttctgga agactttgag tttgcgccag
     1501 aaattattca gtccggattt tataagcacg tttattcgtc cggtttcgga cagtttggcg
     1561 gtgaacccat cgctgccgtg ctggacgctt atgagttcaa aaataccgcg cctgacatga
     1621 aactgctgca atacgtcagt gccgtgggcg caatggcgca cgcaccgttc ctgtcctccg
     1681 tttccccgga gtttatgggg ctgaactcgt ggaccgaact gcctaatatc aaagatcttt
     1741 atgccatctt tgaaggtccg gcctacacca aatggcgcgc cctgcgtgac tcggaagatt
     1801 cgcgctattt agggcttacg gcaccccgct tcctgcttcg ccagccctat tcaccaacag
     1861 ataaccctgt taagaacttt aactattacg aagatgtcag ccagaaccac gaagattatc
     1921 tgtggggtaa tacggcctgg atgctggcat gcaatatcgc agacagtttt gccaaatacc
     1981 gctggtgtcc aaacattatc ggtccgcaaa gcggcggcgc agtgaaagat ctgccggtgc
     2041 atctgttcga aacgatgggg cagattcagg ccaaaatccc aaccgaggtg ctggtcaccg
     2101 atcgccgcga atttgaactg gctgaagagg gttttatcac ccttaccatg cgtaaagact
     2161 ctgataatgc agcctttttc tctgcaaact cggtacaaaa accgaagcac tttccgggaa
     2221 aagatgcaga aaccaactat aagctgggta cgcagcttcc ttatctcttc atcatcaatc
     2281 ggttagcgca ctacatcaag gtgttgcagc gtgaacaact ggggtcatgg aaagaaagga
     2341 gcgacttaga aagagagctt aatacctgga tccgtcagta tgttgccgac caggaaaacc
     2401 cgcctgcaga cgtgcgcagc cgcaaacctc tgcgtgctgc caaagttgag gttatggatg
     2461 tagaaggcga acccggctgg tactaggttg cgctaagcgt gagacctcat tttaaattta
     2521 tgggggcaaa ttttgagctt tccctggttg gccggttaga cagggagtaa cacaccatga
     2581 tactggatcg ggaaagaacc acaggttctc tttttgagcg tatggaagcc tcttcggcca
     2641 gaaaccggca gggaggcagt attcattcgc tcaggcaatc cattcgccag aacctaagaa
     2701 acattcttaa tacccggtcc ggtagttgcc ggggtgcgcc tgagctgggc attgatgaac
     2761 cggaaggggc tgaaaacttc cgcgaatcca tgagtcgggc catcgagcaa tgtatagaac
     2821 gctatgaacc ccggatctca cacgctgaag tgcaggcagt ggtttcatcc gcctccagcc
     2881 cgctggatat gacgtttcat attacagcct gggtgacatt taacgaaacg catgaggtgc
     2941 ttgaatttga tatggcaccg aatggcagcc agcattaccg ggtggactga caaatggaat
     3001 ttgaagaacg ctattttcgg gaagaactcg attacctgcg ccagcttagc aagctgctgg
     3061 caacggaaaa accccatctg gcccgcttcc tggccgaaaa agatgcggat ccggatattg
     3121 aacgcctgct ggaaggggtg gcttttctta ccggcaatct ccgccagaaa attgaggatg
     3181 aattcccaga actgacgcac gggcttatta agatgctatg gcctaattac ctgcgtccgg
     3241 ttccggcaat gacccttatt gaatatacgc cggatatgga taagtcttct gtaccggtgt
     3301 taatcccccg taatgagcag tttacaacca acgccgggga aatcagagtt gatgaagtgc
     3361 tgccctctga tgctaaaaag gaggagccgc ctccctgtac cttcaccctc tgccgggata
     3421 tctggctgct gcccgttcgc ctggagcaga ttgaaaaccg cagtacgacc cgtaatggtg
     3481 ttatcaacat caccttttcg gtcgcaccgg gaacggactt ccgcacgctg gatctgaaca
     3541 aacttcgctt ctggctcggc aatgacgaca actatacccg tgaccagctt tatttatggt
     3601 tctgcgaata cttgcagggt gccgacctga ctgtgggtga acagcatatt cgcctgcctg
     3661 agtttatgct aaaagctgtc ggttttgagc cgcaggatgc catgctgccc tggccgaaaa
     3721 acgtccacag cggctaccgg atccttcagg agtatttctg ttaccccgat gcgtttctct
     3781 tttttgatct ttgtggttgt ccggctttgc ctgacggatt gcaggcggaa ttctttaccc
     3841 tgcaactgcg tttttcgcgc cctttgcccg tggacaaccg gcgggcagac tatccgctgg
     3901 ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt gtggtgagcc
     3961 aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg caggccgcgc
     4021 gccagtggcc agcctttgaa agtttcagcc atcagatgga atacagccgg aagcgggagg
     4081 tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt gatcataccc
     4141 ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc agtaatgaag
     4201 tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt cgttccggcg
     4261 atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc ctttcgcaac attacccgcc
     4321 cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggtccctg ctctccgcca
     4381 tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc atcgccaact
     4441 ttgatcgcca cgcaatccac catccgcaga cggcgcggct gtcacaacaa aagctggatg
     4501 ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt cccgtccggg
     4561 gactggcctc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg gaaatgtatc
     4621 tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac tcattccaca
     4681 tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa agaatcgggc
     4741 agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga atgatccgct
     4801 ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg agctgttgca
     4861 ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgcgacg atccggcaca
     4921 ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg atatcagtgc
     4981 gctgaaacgg gatacgtcag gggcattcag gatgaccact acctttatgg ggttacaggg
     5041 gagccagtcg cctctccccg gttattacct cgatcacctg gcctggaaag cggtacatga
     5101 acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc agtttgtctg
     5161 gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg tggatgcgtt
     5221 ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg ataagctggc
     5281 gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg gacgatcccc
     5341 ggaaattatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga cgctgcaaaa
     5401 ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aacagcctgg ggagctattc
     5461 tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg ttctgggtac
     5521 acgagtaccc gatctcagcg gaaaattcca gctcagtatt accagcctga cccgaaaaca
     5581 gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgacgt tcgtgtcctt
     5641 catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg agcaggtggg
     5701 cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct tcctcggcac
     5761 accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga taatcacgga
     5821 gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta acggcagtga
     5881 gctggaaagc gggcgggcgg cgcgctgtct gttcacacag cagggaaatg tcggccatgc
     5941 ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc aggcttttac
     6001 cgttatcctg cacgatggca cattttgtct acgcccgcag acggcacaac tgtggctgaa
     6061 tcaggcaaaa gtcacagcaa catcagacct gatacagttg cgccagggcg atgagatcca
     6121 gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc attacgatga
     6181 ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg ataccctgct
     6241 atcaacggag ggtgcgccac actatccggg aatgactcac cggcaccagc ttgcagacac
     6301 cgtggtaaat ggtttttctg ccgatccact ccaggcactt cagtccgaaa gcctgattac
     6361 cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc tgtccgatcc
     6421 ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt atgccagccc
     6481 tggcgatcat aatgatgaca tctctgcggc agaaatggcg caacgccacc ttgcggtcac
     6541 ccccttactg cgcggtcttg gcggctcgct taccgtgagc aattccgacg atgcggatga
     6601 ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc tcgatttgca
     6661 gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata acccgctgcg
     6721 cctgaacatg gattacgcca ccgcgctcga cgtgatgttt gccgaaggta aaagcccggt
     6781 acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc accacgaaga
     6841 agctaaccgg gcggcgattg tggagtcgct tcgtgtcctg ctggatgctt tctcaccaca
     6901 aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc agccgctgga
     6961 tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct ccgatcgcca
     7021 gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc gggtgcttcg
     7081 tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg cgtgctgatg
     7141 tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gcgaggtgat caaaaacccg
     7201 gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac cctgctgact
     7261 gaaccggaca ccaataccaa tgcggagggt gaatcagccg ccgtggatgt gcaactggtg
     7321 tacctgactg acgactcaaa attgcaggca gccgactatg accagatagc cagtacgccg
     7381 ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct gttgccggac
     7441 accatcaaaa ccctgccgcc gataaagctg gatgagaaaa cgcagtttat tggtgtcgtg
     7501 gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt ggaaggaacc
     7561 ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat gaaaaaagag
     7621 gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg atgctgatgc
     7681 gcccacacca tttccagcaa cagcagcgtt acaacgacta cctggataac cagcgtttcc
     7741 gggccatgaa tgatttatcc tggggattta ccgaactcac cctcaacaat gaactgctgg
     7801 cgcagggtaa gatcatgatt gacagcgcgt caggcacact gcctgacggc accgtctttt
     7861 ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaaaatttt ccggacgaaa
     7921 gaagccgcaa tatttacctc gctctgccgg tcgccagtga tgtgagaaat gaaatcagcg
     7981 acgggcggcg aatcgggcgt taccggctga attatgccga tgtccgggat ctgcattcag
     8041 aagaaggcga cgcgcgaaca ctgacgctgg gacaactgac gccgcgcatt atgagcggtg
     8101 cagaagatat gagtgcctat attacgctcc cgctttgccg tatcagcgat cgccatgccg
     8161 acggctccct gacgctggat gacgatttta tcccctcctg ccagaatatt caggtcagta
     8221 agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg gcaagcgatc
     8281 tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg gaatttatga
     8341 tgttgcagtt gcttaaccgt aaccagaccc ggtttaccca tcgcgcccgc cgatcccagc
     8401 tccacccgga agatttctac cttgatcttg ccgagttgct gggtgaactg atgaccttta
     8461 cagagccgtc acgcctgccc tgcccgcttg atgtgtatga tcatcatgac ctgaccaaaa
     8521 catttaaaac actgttaccg gaagtcaaac gggcgctgca taccgtactg tcgccaagag
     8581 cggtcaatct gccgctgcat ctgcgtgacg gcatctggca ggccgatgtt catgactcgg
     8641 aactgctaca gtctgccacc tttgtactgg ccgtggcagc aaatatgcct gtcgatcaga
     8701 tccagcgtca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc cgcaatatgg
     8761 tcagtgtgca gatcccgggt attcctctgc gcgcactgat ggtggccccc cgccagcttc
     8821 cctaccattc cgggttcagc tatttcgaac tcgacaagag cggccaggcc tggacagaaa
     8881 tggctgccgc cggggccgtt gcactgcatg tatccggcag tttcccggat ctgaacatgc
     8941 aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct gccaccattt
     9001 accggatacg acgaaaaaag cctgcgcgac taccatctcg cgcttcatgg caacagcctg
     9061 aatccgatga tcgacgcggc gacgccgcta ctcggtatgg taatgcgcct ttccaccatg
     9121 aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt tcaggccgtg
     9181 gagcaactgc ttcaggaaca gggctacgaa ccgggcgtga ttatctcgtt tcgctacatc
     9241 ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcaaacaa gaacgagtgg
     9301 atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga gaaagttttc
     9361 atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct ggaatttctc
     9421 tggctctgtt tttctctggg atttcgcggg cgctacaaag tggcagtaca ggatcagggg
     9481 gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg cggcgatgcc
     9541 cctttcccgc tgttacacca ggacaaaaaa acccaaggcg ggcgttatca gctcatcagt
     9601 cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt gttttacctg
     9661 ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct aaacaaatta
     9721 cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa cggctgaacc
     9781 tgttctcccg ccaggcgctg gagatggccg cctcagaatg tatgagccag caggcagcgg
     9841 aaattaccgt cagccatgtg cttattcaga tgctcgccat gccacgcagt gacctgcggg
     9901 ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgttg acggtagaga
     9961 actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg ctggttgagt
    10021 ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca gcacagcgaa ctgcgcggcg
    10081 gcgtgttgct gctggccttg ctgcattcgc cgctgcgtta tataccgcct gctgccgccc
    10141 ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag tggacacagg
    10201 agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca ctgacggacg
    10261 cctctgacac cctgcttgcc cgctacgcca aaaacatgac agaagacgcc cgtaacggca
    10321 ggctagcccc agtactgtgc cgcgaccacg aaatcgacct gatgatcgac attctctgcc
    10381 gccgccgtaa aaacaacccg gtggtggtgg gcgaagcagg cgtgggtaaa agcgcgctga
    10441 ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaaactg aaaaacaccg
    10501 atatcatgac ccttgacctg ggcgcattgc aggccggggc atcggtgaag ggtgagtttg
    10561 aaaaacgctt caaggggctg atggcggagg tcatttcctc tccggtgccg gtcattctgt
    10621 ttatcgacga agcgcatacc ctgattggcg cgggcaacca gcagggcggg ctggatatct
    10681 ccaacctgct gaaaccggcg ctggcgcgtg gcgaactgaa aaccatcgcc gccaccacct
    10741 ggagcgagta caaaaaatac ttcgagaaag acgccgccct gtcgcgccgc ttccagttag
    10801 tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgtggt ctgtcggcgg
    10861 tctatgaacg gtctcacggc gtgctgattg acgatgatgc cttgcaggcc gcagcaacgc
    10921 tgagcgaacg ttatctctcc gggcgtcagt taccggacaa agcgattgat gtgctggata
    10981 ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc tcggcgctga
    11041 ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag cttcgcatcg
    11101 gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat gaaacgctga
    11161 cggcgctgga tgaactggaa gcggcttggc accagcagca gacgctggtc cgggagatta
    11221 tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg ttgccggacg
    11281 cagatactgt ggaggatacg cagccagagt cagagtcaga gtcagaacag gataataccg
    11341 gtgccgtacc ggctgatgag accgacagag aacagccgga agagaccgct gaaacagttt
    11401 ccccggtaca gcggctggcc cagctcactg ccgaactgga cgccctgcat aacgaccggt
    11461 tgctggtctc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc gaatggaccg
    11521 gcgtgccgct caaccgcctg tcgcagaatg aaatgtcggt catcaccgac ctgccgaaat
    11581 ggctgggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcat aagcatctac
    11641 tgaccgcacg cgccgacctg cgtcgtccgg gacgcccgct cggtgcgttc ctgctcgctg
    11701 gccccagcgg cgtgggtaaa accgaaaccg tcctgcaact ggcagagctg ctctacggcg
    11761 gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacac accgtttcgc
    11821 ggctgattgg ctcccctccg ggctatgtcg gctatggcga aggcggcgtg ctgactgaag
    11881 ccatccgcca gaaaccctac tcggtggtgc tgctcgatga agtggaaaaa gcgcacccgg
    11941 atgtcctcaa cctgttctac caggcgtttg acaagggcga gatggccgac ggcgaaggcc
    12001 gcctgattga ctgtaaaaac atcgtcttct tcctcacctc caacctcggt taccaggtga
    12061 ttgtcgaaca cgcggatgac ccggaaacca tgcaggaagc cctctatccg gtgctggcgg
    12121 acttctttaa acctgccctg ctggcgcgaa tggaggtggt gccgtacctg ccgctgtcga
    12181 aagagacgct cgccaccatt atcgccggga aactggcccg tctggataac gtgctgcgca
    12241 gtcgctttgg tgcagaagtg gtcattgaac cggaagtgac ggacgaaatc atgagccgcg
    12301 tcacccgcgc ggaaaacggc gcgaggatgc tggaatctgt catcgatggc gacatgctac
    12361 cgccgctctc gctgctgctg ttgcagaaaa tggcggctaa cacggcgatt gcccggattc
    12421 ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag aacgacgagt
    12481 ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc agacaggatg
    12541 gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc
    12601 aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga cagccacgtt
    12661 acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg actgctatga
    12721 ccacctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca aagccggttt
    12781 tgtgggcgag gcctggaccc gcgccacaga gcaggaaaaa cgccgcgagg gcaacaccac
    12841 agaactgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa ccaccccggc
    12901 tatcggccat gttccgccgc gcccggtgct gatgttcagt tgcgtggaca atatcacccg
    12961 gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca ctctgaatgc
    13021 cgacagccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc tgctggagtc
    13081 cagccggggg ctgtccggga ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac
    13141 agtggatacc ggcgcagata acgccgcagg aaagctgacc tttaacattg acgggctggc
    13201 aagggcgatt gcccccctgc gcgatgcctg ccactgggcg ggagaataaa aatatggcaa
    13261 tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg ccggaagaaa
    13321 aactggcatc ggcgctgaaa gatgacaacc cggactggga gtatatcgac ggcgaaatcg
    13381 tcaagctggg gtctctggcc cacgctcagc ttgatattcc cgaactacaa cgcagggggc
    13441 tacagcttct ggcttctgaa agcaaagact tcaggctact ggcacacctg ctcagaaccc
    13501 tgcaacatgc cggtgatcca ctgctggcac tgcacctgct aacgctatac gtggaacatt
    13561 actggactgt ggccgcgccg cagaatatgg cgcataaaaa acgctttgcc agccagatca
    13621 ttaaacgttt tgaaacgggt attgaaggct tttcacaaaa cgctgccaca acgcagcgcg
    13681 atactctgct gggtgagctg gcgaaactgg cgcagtgctg gcagtcacat aacgtcccgg
    13741 aactggcaca ggctaccgat gatctttttg ccctgtacca gcgtacgttt aatcgtgcgg
    13801 ctcctgctcc ggtccccact ccggcggcct ccggtagttc accacaaacc accgtcacgt
    13861 ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca aatcgccatc gacagtcacg
    13921 acgacaaagc ctggcgcgac acgctgttaa aagtggcggc tattttatgt gaacgccagc
    13981 cggactcgcc gcagggctat cgcctgcgcc gccatgccct gtggcaatcc atcaccagta
    14041 caccccaggc ggaaagcgat ggacgtaccc cactggctgc ggtctctgcc gatatggtgg
    14101 cggattacca gtcccggctt gccagcgcgg atatggcgct gtggcaacag gttgagaaaa
    14161 gcgtattgct ggctccttac tggctggacg gtcactgtct ttctgcacag acggcactgc
    14221 gtctgggtta caaacaggtg gcagacacca tccgcgatga ggtcatccgc ttccttgagc
    14281 gtctgcccca gcttaccggg ctgctgttta atgaccgcac accgtttctc agtgagcaga
    14341 cgaaacaatg gctggctgct tcgcccgacg gcaaagttgc accggttgcg caaatcggtg
    14401 aggaatcgca ggcagccaga gcctgttttg ctgggcaggg tctggaggcg gcgctgcgat
    14461 atctggacat gctacccgaa ggcgatcccc gcgatcagtt tcaccgccag taccttgccg
    14521 cacagttgac ggaggaggcg gggctgatac agcttgcgca gcaacagtac cggatgttgt
    14581 tgatgatagg gagtcagatg atggtgtctg actgggagcc atcattactt acgcagcttg
    14641 aacaaaaatt cacggcagaa caataaaaga gaacaggagt ttttgtgttc agattaccaa
    14701 caccccgact actcagcgga ctcaaatcag ccctgcgacc ggcgatgccc cggtttaaag
    14761 tctccgcttt ctggctgctg atactggcgt ggatttttct gcttgctgat actggcgtgg
    14821 atttttctgc tggtatggat ctggtggaaa ggcccgacgt ggacgctgta tgaagaacag
    14881 tggctcaaac cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg
    14941 gtgtggctta ccgtccgggt aatgaagcgc ctgcaactgc ttgaaaaaca gcaaaaacag
    15001 cagcgcgaag aggccattga tccgctcagt gtggagctga acgcccagca gcgttacctt
    15061 gaccgctggc tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagtta
    15121 ccgtggtata tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg
    15181 tttccgtcag acattattta tgccccggag ggcgcgcgcg gcacagaaca acacctgtac
    15241 cttacgcccc atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct
    15301 cccgctgatg cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa
    15361 gaaaagcgcg cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg
    15421 ctgaccgcag acaaacgcca ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag
    15481 gatatacgcc agcatcttca ctgccagtta ccggtttacg tggtactgac ccggcttgat
    15541 ttattacagg gttttgccgc cctgttccag tccctgaaca gacaggatcg cgatgcgatt
    15601 ctgggcgtca cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat
    15661 gctttctggc agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcacc
    15721 cagactcaca cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa
    15781 ccgctggtgt cactgcttga gggtctgctt gacggtgaaa atatgaacgt gatgctgcgt
    15841 ggtgtctatc tcacctcttc gcttcagcgc gggcagatgg atgatatatt cacccagtct
    15901 gccgcccgcc agtaccgact gggcaataac ccactggcgt cctggcctct ggtggacacc
    15961 gcgccttatt tcacccgcag cttgttcccg caggcattac tcgcagagcc taatctggca
    16021 acggagagcc gcgcctggct gatgcgttcc cgtcgccgcc tgacggtttt ctctgccacc
    16081 ggcggcgtgg cggcactgct gctcatcacc ggctggcatc actattacaa cggtaactat
    16141 cagtccggca tcaccgtgct taagcaggcc aaagccttta tggatgtgcc gcctccacag
    16201 ggggaagatg actacggcaa cctgcaactg ccgctgctga acccggtacg cgatgccaca
    16261 ctggcctatg gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga
    16321 cgacgtatcg gaccttatgt ggaacagacc tatctgcaac tgctggagca acgttacctg
    16381 ccctcgctgt ttaacgggct ggttaaagcg atgaatgccg cgccgccgga gagtgaagaa
    16441 aaactcgcgg tgctgcgcgt gatgcgaatg ctggaggaca aaagcggacg taacaatgag
    16501 gtggtgaagc agtatatggc aaaacgctgg agcgaaaagt tccacggcca gcgcgatatc
    16561 caggcacaac tgatgtccca tcttgactac gcgctggctc atactgactg gcacgcagag
    16621 cgtcaggcgg gcgacggtga cgccatcagc cgctggacgc catatgacaa gcccgtggta
    16681 tcagcacaga aagaactgag caaactgcct gtctaccagc gggtttacca gagcctgaaa
    16741 acgcgggcgc tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt agggccaacc
    16801 tttgaccagg tgtttacatc tgccgatgac aacaaactgg ttgttccaca gtttcttacc
    16861 cgttacggcc tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga actgacggcg
    16921 atggattcct gggtacttaa ccttacccgc agcgtgaaat acagtgacgc cgaccgcgcg
    16981 gaaatccagc gccagctcac cgagcagtat atcagcgact acaccgccac ctggcgggcc
    17041 gggatggaca atctgaatat ccgcaatttt gagtccatcg gacaactgac cggggcgctg
    17101 gagcaggtta tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct gcgtgacaac
    17161 acacagccag gcgtcttttc tgaaaaactc tctgccaaag aacgggatga ggccctggca
    17221 gagccggact accagttact cacccgtctc gggcatgagt tcgccccgga aaacattacc
    17281 ctggcagtac agaaagacaa agaaagcacg atgcaggccg tgtatcagca actcaccgag
    17341 ttgcaccgct acctgctggc aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa
    17401 gccgtgcagt tacggcttga tcagaacagc agcgatccga tattcgccac ccgccagatg
    17461 gcaaaaacgc tgcctgctcc gctcaaccgc tgggttggca gactgactga ccaggcctgg
    17521 catgtggtga tggtggaggc tgtacattat atggaagtgg actggcgcga cagcgtggtg
    17581 aaaccgttta acgagcaact ggcaaataac tatccgttta atccgcgttc tgcacaggat
    17641 gcctcactgg atgccttcga acgcttcttt aaaccggatg gcatactgga taccttctac
    17701 cagcagaacc tgaagctgtt tatcgataat gacctgagtc tggaggatgg cgataacaac
    17761 gtcattattc gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc
    17821 ttcttcagca aacagaacgg tctgggaaca tcctttgccg tggaaacggt atcgctttca
    17881 ggcaataaac gccgcagtgt actgaacctt gacggtcagt tagtcgatta cagccagggc
    17941 cgtaactata ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag
    18001 ctgacgctca tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg
    18061 gcgcagttcc gcctgttcgg ggccggacaa ctgaccggag tacaggatgg caactttacc
    18121 gtgcgcttta gcgtggacgg tggcgcgatg acctaccgtg tgcataccga cacggaagat
    18181 aacccgttca gcggtgggtt gttcagccag tttggtctgt cagacacact gtactgagga
    18241 ggcagggatg aatagtaacg tactgacaca aactatcgtt accggcagtg acccgcgcgg
    18301 gctgccggaa ttcagcgcca tccgcgagga aataaacaaa gccagccacc cgtcacagcc
    18361 tgagctgaac tggaaactgg tggagtcgct ggcgctggcg atttttaaag ccaacggtgt
    18421 ggatttacac accgccacct actatacgct tgcccggaca cggacacagg gactggcggg
    18481 attctgcgaa ggtgcggaac tgctggcggc aatggtaagc cacgactggg ataagttctg
    18541 gccgcagggc ggcccggcgc gtactgaaat gctggactgg tttaactccc gaaccggcaa
    18601 tattctgcgt cagcaaatct cctttgcgga atccgaccta ccactgatct accgcacaga
    18661 gcgggcattg cagcttatct gcgataagct ccagcaggtg gaactgaagc gcgttccgcg
    18721 cgtggagaat ctgctctatt ttatgcagaa cacacgtaaa cggcttgaac cccagctgaa
    18781 gagtaacact gagaacgccg cacagaccac ggtcagaacg ctgatttatg ccccggaaac
    18841 acaggcatct tccacaccag aagcggtagt gcctcccctg cccggcctgc ctgagatgaa
    18901 agtggaagtg cgcagtctga cagagaatcc cccacaggcc agtgtgataa agcaaggcag
    18961 tacggtaaga gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg
    19021 gtggtggcag gtctatccgg tgcagcagca actgttacag gttaacgaca ccgctcaggg
    19081 cgcagcaacg gtgtggatgg cctcacctga actcgaaaac tatgagcgca ggctgcaaca
    19141 acttcttgat acctccccgg tacagccgct ggaaaccggg atgcagatga tgcgtgttgc
    19201 cgacagtcgc tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc
    19261 actcaaaacc cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga cccgccagga
    19321 cttacatgct tttgcagatc tagtgatgca gcgcgagaaa gagggactaa ccctttccta
    19381 tatcaaaaat gtcatctggc aggcggagcg gggactgggg caggaaacac ccgttgagtc
    19441 tctgttgacg cagtaccagg atgcccgtgc gcagaagcag aatacagatg cgctggaaaa
    19501 acaaattaat gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga agaataatac
    19561 gataccgacg ataaaaaaag cattgaattt caataacatt catgaataca aaggagtttt
    19621 aaatggcgaa tttaatttat ttaacactaa atggtgataa tcaagggctc atttcaagtg
    19681 gttgttcatc acaaccatcc attggaaata aagctcaaac agcacacatt gatcaaatta
    19741 tgatttatga attcatgcat ggattaaatc gagatcaaaa cgtcaaccat catcatttg
//
LOCUS       DAZGXS010000082        19787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGXS010000082 DAZGXS010000000
VERSION     DAZGXS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(101..307)
                     /gene="ybfA"
                     /locus_tag="ACHKG5_003091"
     CDS             complement(101..307)
                     /gene="ybfA"
                     /locus_tag="ACHKG5_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0869147.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            620..709
                     /gene="kdpF"
                     /locus_tag="ACHKG5_003092"
     CDS             620..709
                     /gene="kdpF"
                     /locus_tag="ACHKG5_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0869148.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            709..2382
                     /gene="kdpA"
                     /locus_tag="ACHKG5_003093"
     CDS             709..2382
                     /gene="kdpA"
                     /locus_tag="ACHKG5_003093"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0869149.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2405..4453
                     /gene="kdpB"
                     /locus_tag="ACHKG5_003094"
     CDS             2405..4453
                     /gene="kdpB"
                     /locus_tag="ACHKG5_003094"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0869150.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4462..5034
                     /gene="kdpC"
                     /locus_tag="ACHKG5_003095"
     CDS             4462..5034
                     /gene="kdpC"
                     /locus_tag="ACHKG5_003095"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0869151.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5027..7711
                     /gene="kdpD"
                     /locus_tag="ACHKG5_003096"
     CDS             5027..7711
                     /gene="kdpD"
                     /locus_tag="ACHKG5_003096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0869152.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7708..8385
                     /gene="kdpE"
                     /locus_tag="ACHKG5_003097"
     CDS             7708..8385
                     /gene="kdpE"
                     /locus_tag="ACHKG5_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0869153.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
     gene            8547..8681
                     /locus_tag="ACHKG5_003098"
     CDS             8547..8681
                     /locus_tag="ACHKG5_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0869154.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8660..8764
                     /gene="speFL"
                     /locus_tag="ACHKG5_003099"
     CDS             8660..8764
                     /gene="speFL"
                     /locus_tag="ACHKG5_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP0869155.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9075..11273
                     /gene="speF"
                     /locus_tag="ACHKG5_003100"
     CDS             9075..11273
                     /gene="speF"
                     /locus_tag="ACHKG5_003100"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0869156.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDKSNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11270..12589
                     /gene="potE"
                     /locus_tag="ACHKG5_003101"
     CDS             11270..12589
                     /gene="potE"
                     /locus_tag="ACHKG5_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0869157.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12646..14286)
                     /gene="pgm"
                     /locus_tag="ACHKG5_003102"
     CDS             complement(12646..14286)
                     /gene="pgm"
                     /locus_tag="ACHKG5_003102"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0869158.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14312..14857)
                     /gene="seqA"
                     /locus_tag="ACHKG5_003103"
     CDS             complement(14312..14857)
                     /gene="seqA"
                     /locus_tag="ACHKG5_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0869159.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15042..15806
                     /gene="ybfF"
                     /locus_tag="ACHKG5_003104"
     CDS             15042..15806
                     /gene="ybfF"
                     /locus_tag="ACHKG5_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0869160.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15946..16239
                     /gene="ybfE"
                     /locus_tag="ACHKG5_003105"
     CDS             15946..16239
                     /gene="ybfE"
                     /locus_tag="ACHKG5_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0869161.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16379..16909
                     /gene="fldA"
                     /locus_tag="ACHKG5_003106"
     CDS             16379..16909
                     /gene="fldA"
                     /locus_tag="ACHKG5_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0869162.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17119..17205
                     /gene="uof"
                     /locus_tag="ACHKG5_003107"
     CDS             17119..17205
                     /gene="uof"
                     /locus_tag="ACHKG5_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0869163.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17198..17644
                     /gene="fur"
                     /locus_tag="ACHKG5_003108"
     CDS             17198..17644
                     /gene="fur"
                     /locus_tag="ACHKG5_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0869164.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17728..18054)
                     /gene="chiQ"
                     /locus_tag="ACHKG5_003109"
                     /pseudo
     CDS             complement(17728..18054)
                     /gene="chiQ"
                     /locus_tag="ACHKG5_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
     gene            complement(18104..19509)
                     /gene="chiP"
                     /locus_tag="ACHKG5_003110"
                     /pseudo
     CDS             complement(18104..19509)
                     /gene="chiP"
                     /locus_tag="ACHKG5_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         4555 a         4982 c         5260 g         4990 t
ORIGIN      
        1 ttggtataac ggtgtctaaa tattcctctg aacatcttcc tgatacaaaa aaccgccagc
       61 gtttccgctg gcggttttca atgtattcaa aactcagcaa tcagtaaaaa tcaccggttg
      121 ccttttcggc ctgatccatc caaaccggtt tatcgctggt tttcgaccag acgcgatgca
      181 ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc
      241 ccgctgcaac cgcataagta cggcgtaaaa agataagcca tgcaggatat tctctgtaga
      301 gttccatact tttctccccc ttttgtatct acccggtgaa tagcaccgga aaaatgaatt
      361 tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt
      421 atttttgctt attgatggtt tatttacatt catcctgtaa ttaagttaca caaaagttaa
      481 attaatacta aacattagtt aaatcatggc ttttgccatt tttatacttt ttttacaccc
      541 cgtccgcaga tttttgcgaa atctttgcag ccagaattct acccttccgg tatcactttt
      601 aggccactgg aggtgcactg tgagtgcagg cgtgatagcc ggcgtgttgc tggtgttttt
      661 attactgggt tatttggttt atgccctgat ccatgcggag gcattctgat ggctgcgcaa
      721 gggttcttac tgattgccac gtttttactg gtattaatgg tgctggcgcg tcccttaggt
      781 agcgggctag cgtggctgat taatgatatt cctcttcctg gtacgacggg cgttgaacgc
      841 gtactcttta gcgcgcttgg cgtctctaac catgagatga actggaagca gtatctctgt
      901 gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt gctcggtcag
      961 cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct ggcgctgaat
     1021 accgccgtca gctttgtcac gaataccaac tggcaatctt atagcggtga aaccacgttg
     1081 agctatttca gccagatggc gggcttaacg gtgcaaaact ttctttctgc cgccagcggg
     1141 attgcggtga tttttgccct cattcgtgca tttacccgcc agagcatgag cacactcggg
     1201 aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tcgtccctgt ggcgttggtg
     1261 atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttctgcccta tcaggcagtg
     1321 aataccgttg aaggagcgca acagctgtta ccaatgggtc cggtggcttc tcaggaagcg
     1381 atcaagatgc tcggcactaa cggcggtggt ttctttaatg ccaactcgtc gcatccgttt
     1441 gaaaacccaa ccgcactgac caacttcgtg cagatgctgg cgatcttctt gatcccaaca
     1501 gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc gtcaggggcg catgttgctg
     1561 tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg tgatgtgggc agaagttcag
     1621 ggtaatcctc atctgctggc gctcggcgca gacagcagca tcaatatgga aggtaaagag
     1681 agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc ggcgtcctgt
     1741 ggcgcggtga ttgcaatgca tgattcgttt accgctctcg gtggcatggt gccgatgtgg
     1801 ctgatgcaaa ttggtgaagt ggtattcggc ggtgtcggtt ctggtcttta cggcatgatg
     1861 ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac accggaatat
     1921 ctgggtaaaa aaatcgacgt acgtgagatg aaactgactg cactggcgat tctggtcacc
     1981 ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga ccgacgccgg acgtagcgcc
     2041 atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc atccgccgct
     2101 aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg gaactgttta
     2161 ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat ggcaattgcc
     2221 ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacgctgcc aacgcacggc
     2281 ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact gacctttatc
     2341 cctgccctgg cgcttggtcc ggtggcggaa tatctctcct gatgatattg agtgagcact
     2401 gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa
     2461 agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga tgtttatcgt
     2521 ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg gcgcgatgcc
     2581 tggcaatgcg ctgtttagcg tggccattag cggctggctg tgggtcaccg tactgttcgc
     2641 taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg caggccaaca gcctgaaagg
     2701 ggtgaaaaaa acggcctttg cccgcaagct gcgtgagccg aaatatggcg ctgcggcgga
     2761 caaagttccc gccgatcaac tgcgtaaagg cgatatcgta ctggtagaag caggcgatat
     2821 tatcccctgc gatggtgaag ttattgaagg cggcgcatcg gtcgatgaaa gcgccatcac
     2881 cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg tcaccggcgg
     2941 cacgcgtatt ctttctgact ggctggtgat tgagtgtagc gttaaccccg gcgagacatt
     3001 tctggatcgg atgatcgcga tggtggaagg cgcacagcga cgcaaaacgc cgaacgagat
     3061 tgctctgacc atcctgctga ttgccctgac tatcgtcttt ttactggcaa ccgccacgct
     3121 gtggccgttt tccgcgtggg gcggtaatgc agtcagcgta acggtactgg tggcgctgct
     3181 ggtctgtctg atcccaacca ctattggcgg cctgttgtca gcgatcggcg tcgccgggat
     3241 gagtcggatg ctgggcgcga atgtggttgc caccagcgga cgtgcagttg aagcggcagg
     3301 tgacgttgac gttctgctgc tggataaaac cggcaccatc acactcggta accgtcaggc
     3361 atcggagttt atccctgcgc agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact
     3421 ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg ccaagcagcg
     3481 ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac cgtttactgc
     3541 gcaaagccgg atgagcggga tcaatatcga caaccgcatg atccgtaaag gttctgtcga
     3601 tgccattcgc cgccatgttg aggctaacgg tggtcacttc cctgccgatg ttgatcaaaa
     3661 agtcgatcag gttgcgcgtc agggggccac accgctggta gtagtagaag gttctcgtgt
     3721 gctgggcgtt attgcgttga aagatatcgt caaaggcggc attaaagaac gcttcgccca
     3781 actgcgcaaa atgggtatta aaacggtgat gattaccggc gataaccgtc tgactgccgc
     3841 cgcgatagct gcggaagcgg gcgtcgatga ttttctcgcc gaagcgacac cggaggccaa
     3901 gctggcattg atccgtcagt atcaggcgga aggccgtttg gtagcaatga ccggcgacgg
     3961 caccaacgat gctccggcgc tggcgcaggc agatgtcgcg gtggcgatga actccggtac
     4021 tcaggcggcg aaagaagcgg gcaatatggt ggatctcgac tctaacccga ccaagttgat
     4081 cgaggtggta catattggca aacagatgct gatgacccgt ggctcgctga ccaccttcag
     4141 cattgccaac gatgtggcga aatacttcgc cattattccg gcggcattcg cggtaacgta
     4201 tccacagtta aatgcgctga acatcatgcg cctgcattcg cccgactccg ccattctcag
     4261 tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt cccctggcgt taaaaggcgt
     4321 gagttataaa ccgcttaccg cttctgccat gttgcgccgt aacttatgga tttacggtct
     4381 gggtgggctg ctggtgccgt ttattggtat caaagtcatt gatttactac tgaccgtttg
     4441 cggtctggtg tgaggtttac catgcgtgga ttacgtccgg cattatcgac atttatcttt
     4501 ctgttattga ttactggcgg tgtttacccg ctgctgacca ccgcgctggg gcaatggtgg
     4561 tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg atacggtgcg gggttcggca
     4621 ttaatcgggc agaattttac cgacaacggc tattttcatg gtcgcccgtc ggcaacggca
     4681 gaaatgcctt ataatccaca ggcttctggc gggagcaatc tggcggtcag taaccctgag
     4741 ctggataaac taatagccgc acgcgttgct gcattacggg ccgctaaccc gaatgccagc
     4801 acgagcgttc cggttgaact ggtgacggcg tcggcaagcg ggctggacaa taacatcacc
     4861 ccgcaagcgg cggcctggca gatcccacgc gtagcgaaag cgcgtaatct cagcgttgaa
     4921 cagctcacgc aactgatcgc aaaatacagc caacaaccac tggtgaaata tatcggccag
     4981 ccggtcgtca acattgtcga actcaatctg gcactggata aacttgatga ataacgaacc
     5041 cttacgtccc gaccccgatc gtctgctgga acaaactgcc gcgccgcatc gggggaagct
     5101 gaaagttttc ttcggtgcct gcgcaggcgt cgggaagacc tgggcgatgc tggcagaagc
     5161 ccagcgactg cgggcgcaag ggctggatat tgtggttggc gtggtagaaa cccacgggcg
     5221 gaaagatacc gccgccatgc tggaagggct ggctgttctg ccgccaaaac gcctggcgta
     5281 ccgtgggcgg cacatcagcg agtttgatct cgatgccgcc ctcgcccgcc gtccggcgct
     5341 gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt tctcgtcatc ctaaacgctg
     5401 gcaggatatc gaagaactgc tggaagctgg cattgatgtt ttcacaaccg tcaacgttca
     5461 gcatctggaa agtctgaatg atgtggtcag cggcgtcacc ggaattcagg tacgggaaac
     5521 cgtgcccgat ccttttttcg atgccgccga cgacgtggtg ctggtggact tgcccccgga
     5581 cgatctgcgc cagcggctga aagaaggcaa agtctatatt gccgggcagg cggagcgcgc
     5641 cattgaacat tttttccgca aaggcaatct gatcgccctg cgcaaactgg cactgcgccg
     5701 tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg gggcatcctg gcgaagagaa
     5761 agtctggcac acgcgcgacg cgatcctttt atgcatcggc cataacaccg gcagcgaaaa
     5821 actggtccgc gcggcggcgc ggctggcgtc acggctgggg agcgtctggc acgcggtgta
     5881 tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc
     5941 cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc cagcggaaga
     6001 gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc tcggtcgccc
     6061 agcctcgcgc cgctggtggc gtcgggaaac gtttgctgac cgactggcgc gcatcgcccc
     6121 cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc
     6181 gccggatagc cgctctttta aagacaagtg gcgggtacag attcagggat gcgtggttgc
     6241 cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg cgtttgatgc
     6301 cgccaacctg gtgatgctgt atctacttgg cgtggtggtg gtggcgctat tttatggacg
     6361 ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc
     6421 cccacgcggc acgctcgccg tctctgatgt gcaatatctg ctgaccttcg cggtgatgtt
     6481 aaccgtcggg ctggtgatcg ggaaccttac tgcgggtgtg cgttatcagg cgcgggtagc
     6541 ccgttaccgc gagcaacgca cacggcactt atatgaaatg tcgaaagctc tggcggtggg
     6601 ccgcagtccg caggacatcg ccgccaccag cgaacaattt attgcctcca cgtttcatgc
     6661 ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg cagccgttaa cacatccgca
     6721 aggaatgacg ccgtgggacg atgccatcgc gcagtggagt tttgataaag gcctgcctgc
     6781 gggtgcgggc accgacacgt tacccggcgt gccgtaccag attttgccgt taaaaagcgg
     6841 tgagaaaacc tacgggctgg tggtggtgga accggggaat ctgcgtcagt tgatgatccc
     6901 ggaacagcag cgcctgctgg agacgtttac gctgttagtc gccaatgcgc ttgagcggct
     6961 gacgctgacc gccagcgaag aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa
     7021 cgccctgctg gcggcgcttt cgcatgattt acgcactccg cttacggtgc tgtttggtca
     7081 ggcagaaatc ttaacgctcg atctggcaag cgaaggatca ccccacgccc gccaggccag
     7141 cgagattcgt cagcatgtgc tgaacactac ccgactggtg aataatctac tggatatggc
     7201 gcgaattcag tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg aagaagtagt
     7261 cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc tttctctgcc
     7321 agaaccgctg accttaatcc acgttgacgg gccactcttt gaacgggtgc tgattaatct
     7381 gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa attggtatcg atgcccacgt
     7441 tgagggcgaa aatctacaac tggatgtctg ggataacggc cccggtcttc cgccaggcca
     7501 ggagcagacg atatttgata agtttgctcg cgggaataaa gagtcggcag taccgggggt
     7561 agggcttgga ctggcaattt gtcgggcgat agtggatgta cacgggggca ctattaccgc
     7621 gttcaaccgg ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc aaactgcccc
     7681 tgaacttgaa gaatttcatg aggatatgtg acaaacgttc tgattgttga agatgaacag
     7741 gctattcgtc gctttctgcg cacggcgctg gagggcgacg ggatgcgcgt ctatgaggcc
     7801 gaaacgctgc aacgcggctt gctggaagcg gctacccgta agccagattt gattattctc
     7861 gatctcggcc tgcccgatgg tgatgggatt gagtttatcc gcgacctgcg ccagtggagc
     7921 gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga gcgacaaaat cgccgcgctg
     7981 gatgccggag cggatgatta tctaagtaag ccgtttggca ttggcgaatt gcaggcccgt
     8041 ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc ccgatccgct ggtgaaattt
     8101 tccgatgtta ccgtcgattt agccgcccgc gtgattcacc ggggtgagga agaggtgcat
     8161 ctcaccccga ttgagtttcg cctgctggca gtgctgctca acaacgccgg aaaagtgctc
     8221 acccagcgcc aactccttaa ccaggtgtgg gggccaaacg cggtcgaaca cagtcattat
     8281 ttgcgtattt atatgggaca tctgcgacaa aaactggaac aggatcccgc tcgccccagc
     8341 catttcatta ctgaaaccgg tattggttat cggtttatgc tttgaatttt tattcaataa
     8401 attcgcctaa atagtgatta atttattatt aaacctgcgc caaatattat tatttggcag
     8461 gtttaatttc ttaacgcaaa tataaaacac gaaattacaa catttaagta acaaaacagc
     8521 atctagtatg gcgctattca taattaatga tttattatct ggggtatgat cgttttttgt
     8581 tgatcttctt cacagattat agccatttca tggatagaat aactctacct tcaactgaca
     8641 cagcaagagg taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac
     8701 aacccatatc atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg
     8761 ctagtcttct gactaaaggg caccccagcg tacaggtctc cctgacttta agcattacag
     8821 gttaatacct gtatccctcg gtgctcatat actgctaacc cttttaaact ctacataatt
     8881 cgagtcgcag catatgcaac ttgaagtatg acgagtatag ccagttaccg ggctggtctg
     8941 ggttattgca tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt
     9001 tttttccggc gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata
     9061 aagagatgaa caaaatgtca gaattaaaaa ttgccgttag tcgttcttgc ccggattgtt
     9121 tttccactca tcgtgcatgc gtgaatatag acaaaagtaa ttatattgac gttgccgcca
     9181 ttattttatc agtcagtgat gttgaacgtg gaaaactcga tgaaatagac gctactggct
     9241 atgacattcc tgtttttatt gcaacggaaa atgaagaacg tataccagca gaatatcttt
     9301 cacgtatttc tggtgtcttc gaacatggag aagcacgcaa agaattttat ggtcgtcagt
     9361 tagaaaccgc tgccagccat tatgaaactc aactgcgccc acctttcttt cgcgcactgg
     9421 tcgattatgt caatcagggc aacagcgcat ttgactgccc cggtcatcag ggcggcgaat
     9481 ttttccgtcg ccatccggcg gggaatcagt ttgtggaata ctttggtgag gcgctgttcc
     9541 gtgccgactt gtgcaacgcc gacgtagcga tgggcgatct gctgattcac gaaggcgcgc
     9601 catgcattgc acagcaacat gcagcaaaag tgtttaatgc cgataaaacc tacttcgttt
     9661 taaatggcac ttcatcttct aacaaagtgg ttttaaacgc cctgctgaca ccgggtgatc
     9721 tggtgctatt tgaccgcaat aaccataagt ctaaccacca cggggccttg ctacaggctg
     9781 gtgcaacacc ggtttatctg gaaacagcac gtaacccgta tggttttatc ggtggtattg
     9841 atgcacactg cttcgaagaa agttatctgc gtgaactgat tacagaagtt gcaccgcaac
     9901 gggcaaaaga ggcgcgtccg ttccgcctcg ctgtgattca gctaggcacc tacgacggta
     9961 cgatttataa cgcccgccag gtggtggata aaattggtca tctgtgtgac tacatcctgt
    10021 ttgattccgc atgggtcggc tatgaacagt ttattccgat gatggctgac tgttcgccgc
    10081 tgttgctgga tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac
    10141 agcaggctgg tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc
    10201 aacagcgtta tgtaccgcac aaacgcatga acaacgcctt tatgatgcat gcctccacca
    10261 gcccgttcta tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca
    10321 gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc
    10381 tcgataactg tcagcatatt cgtccgttca tacctgaact ggtggatggt aaaccctggc
    10441 agtcgtatga aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccaggtg
    10501 agcactggca ttcttttgaa ggctatgcag agaatcaata ctttgttgat ccgtgcaaac
    10561 tgttgctgac aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac
    10621 ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc
    10681 ttaactccat cctcttcctg ctgactccgg cagaagatat ggccaaactt cagcaacttg
    10741 ttgccctgct ggtacgcttc gaaaaactgc tggaatctga tgcgccgctg gcggaagtgc
    10801 taccttccat ctacaaacag catgaagagc gctacgccgg ttataccctt cgtcagttgt
    10861 gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt
    10921 tccgtaagga acacttccca cgcgtcagca tgaatccgca agaagctaac tacgcctatt
    10981 tacgcggtga agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag
    11041 gtgcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg
    11101 ctgttttgcg ttacttcagc gctctggaag aagggatcaa cctgctgcca ggttttgcac
    11161 cggagctgca gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg
    11221 tcatcaagcc tcgtgatgcg caaagcaccc tgctgaaagg ggaaaaatta tgagtcaggc
    11281 taaatcgaac aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg
    11341 ctccggtatc atcatgctgc cgacaaagct tgccgaagtt gggacaatct caattatctc
    11401 ctggctggtg acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat
    11461 gttcagccgt aaatcaggcg gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg
    11521 taactttatg gcgaactata cctacggcgt ctcactgctg attgctaacg tcgcgattgc
    11581 tatttcggcg gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat
    11641 tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg
    11701 cattaccggg caaatcagta gcattactgt gtggggagtc attattccgg tcgtcggtct
    11761 gtgcattatt ggctggttct ggtttagccc gacgctgtac gttgattcct ggaatccgca
    11821 tcatgcaccg ttcttcagtg cggtaggttc ttccatcgct atgacgctgt gggcttttct
    11881 tggtctggag tctgcgtgtg caaatactga tgtagtggaa aacccggaac gtaatgtgcc
    11941 aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt
    12001 gattgccggg attgtgccaa atatggagct ggcaaattca acggcaccat ttggtctggc
    12061 cttcgcgcag atgttcacgc cggaagtggg taaagtcatt atggcgctga tggtgatgtc
    12121 ctgctgcggt tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc
    12181 tgatgaaggc tacttcccta aaattttctc ccatgtaacc aaagtggatg caccggtgca
    12241 gggaatgttg accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc
    12301 gctaaacagt cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta
    12361 tattctgtca atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa
    12421 agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg tacagctttt atgcgctgta
    12481 ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact
    12541 gtatggtctg gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt
    12601 aaaaaagggc gatcttgcga ccgccctttt tcttatttaa tgtgtttacg cgtttttcag
    12661 aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccgag
    12721 gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca
    12781 gccgttgtca gtcatcactt tcagaccgcc aatagaagca ccgttgcccg gagcagcagt
    12841 caggcgcgcg gtgatcgggt caccagccag ggtgctggcg ctcaccattt ccggagacag
    12901 cttagacagc gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg
    12961 cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc gggttcttac cggtgacagc
    13021 ggtgatttcc gccgccagca gacacatgat gatgccgtct ttgtcggtgg accacggcgt
    13081 gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc
    13141 gtcgaacaga ccatcaacaa accatttgaa acctaccggg acttctacca gtttacggcc
    13201 caagtcgttg accacacggt cgatcatcgc agatgaaacc agcgttttac cgacggcaac
    13261 agctttgccc cactgcggac gatgctggaa caggtagttg attgccaccg ccaggtagtg
    13321 gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt
    13381 agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga
    13441 ggagcagtcc atacggatcg cgccgtcttt atcaaggtgc ataaagcgga aggtttgatc
    13501 gacctgatcg ttaacgatgg tcaggttgag gttgtaatac tcgccaatac gcttccagta
    13561 ttcgataccg gaaccgccca gcggatcaac gccaagcgtc aggcccgctt tctgaatcgc
    13621 cgccatatca acgatatcgg ccagcccttc cacaaacggc tgcaccagat cctgctcttt
    13681 cacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc
    13741 ggccatcagt gcgttggctc tgtcttccac cactttagtg acgttggtat cagccgggcc
    13801 accatttggc ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat
    13861 aacgataccg tctgccagcg ggccaccttt tttattgtga accaggatgg cattggaaac
    13921 ggcaggcgtc ggggtgaagc cattgttttc ctgcacaata acatctacgc cgttcgctgc
    13981 cagcacttcc agaacggaaa taaatgcagg ttcggacagg gcgtgagtat ctttacccac
    14041 atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc
    14101 cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc
    14161 gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt
    14221 cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat
    14281 tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca
    14341 attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg
    14401 tgttggtgtt ggtgatcacc caatacggcg tgcctggcac atgtttcggc ttggtctgat
    14461 taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca
    14521 acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca
    14581 gcataaagcg attgaccgct cgcttttgct ctgcatattc atccgaaagc agaagttcac
    14641 gcattgcgcg aaccttgtct ttaatcgttt tgatcggctt cgcttcgacg atagcaggtg
    14701 acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca
    14761 acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat
    14821 agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag
    14881 ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg
    14941 gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac
    15001 ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg
    15061 cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg
    15121 gcagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc
    15181 aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga
    15241 tggcgcagga tcttgttgat actctggatg cacagcagat cgacaaagca acatttatcg
    15301 ctcactccat gggcggtaaa gcggtaatgg cacttactgc actggccccc gatcgcatcg
    15361 ataaactggt ggcgatcgat atcgcaccgg tcgactatca cgtacgccgt catgatgaga
    15421 ttttctcggc tatcaacgcg gtcagtgaat cggacgcaca aactcgccag caagcagcag
    15481 caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg
    15541 ttgacgggga gtggcgcttt aacgtgccgg tattgtggga tcagtatccg catattgtgg
    15601 gttgggagaa aatcccggca tgggatcacc ctgccctgtt tatccctggt ggtaattctc
    15661 cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag gcacgggcgc
    15721 atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg
    15781 ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct
    15841 ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc
    15901 cgggcagagg cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa
    15961 acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaaca
    16021 aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccaact aaaacgcgac
    16081 aaagtacgtg gcctaaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg
    16141 aacgagctgg cggaatcgcg caatatgagc cgtagcgaac tgatcgaaga gatgttaatg
    16201 cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac
    16261 agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct
    16321 ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta tttcactcat
    16381 ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat
    16441 gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa
    16501 agaagatctg gaagcttatg acattctgct gctgggcatc ccaacctggt attacggcga
    16561 agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt tcaatggcaa
    16621 actggttgcg ctgtttggtt gtggtgacca ggaagattac gccgaatatt tctgcgacgc
    16681 attgggcacc atccgcgaca tcattgaacc gcgcggtgca accatcgttg gtcactggcc
    16741 aactgcgggc tatcatttcg aagcatcaaa aggtctggca gatgacgacc actttgtcgg
    16801 tctggctatc gacgaagacc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt
    16861 taaacagatt tctgaagagt tgcatctcga cgaaattctc aatgcctgat gtgatgcggc
    16921 gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt
    16981 tgtaactttt gccgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta
    17041 aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt
    17101 aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt cacttcttct
    17161 aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca ataccgccct
    17221 aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga
    17281 gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga
    17341 agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt
    17401 cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca
    17461 cgatcacctg atctgcctcg actgcggcaa ggttatcgaa tttagtgatg attccatcga
    17521 agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta
    17581 tctttacggt cactgcgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa
    17641 ataagccagt ctgaacgaga aaagccaacc tgcgggttgg cttttttatg caagggaaat
    17701 aagaaaagcg agagttacag ctctcactta tttgttatta ctgcgaattt cctgccagac
    17761 cttatcacaa tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagacactg
    17821 ctgataatcc agtacgcgga cactttccta gtcggcaaac tgctgatgtt gtttctcttt
    17881 cttcagcacg tttaacacgc tctggcaggc ttcaattttt tccggcgaac cttgtgcggt
    17941 gttgatacag gcgctgtatg cctctttcag acggctgtct tcctgtggcg cggttgattg
    18001 cgcacaagct accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa
    18061 ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg
    18121 gtgcgattac cataaatttt acgtcacgct catcctggaa gatgttgccg taaccgccgc
    18181 cccagcttgg gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc
    18241 ctttggcgcg accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa
    18301 tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat
    18361 cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt
    18421 catacatcgc accgaagaag accgcttttt cgccgttggc gttgaagtca gaacggttat
    18481 cccaccagat atccaggcga ccgttcgagg aagcgtaggt tggagtcata cgttgcagga
    18541 agtatccctg ctgaccgtca gctttaaccc aggtgccttc gaggcgcaaa tccactacgt
    18601 cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaagatcgt
    18661 tgacgctgcg atcgtcaact ttatcgcgcg taccgtagaa ctggtagctg gtggttaacg
    18721 ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa gtattgatcg atataccctt
    18781 ccgcctgacc aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa
    18841 gggagtgcag ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca
    18901 gatgccacgg tgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat
    18961 cgccgtaatc aaaattcgcc ccggcctccg caccctgata agtacccggc ataaagctcc
    19021 agtgcggcgc taacagcgtt tgaccagttg gctgaatata acctgccctc gccacaccgg
    19081 accgtattta aatttggccg ctgctttata caggcttata ccgcttttat cgccggacca
    19141 gtcttcgtca taggctttat tactttttga aaacgcgatt tcgttcgggt gagagctgtc
    19201 gccgttttcc gccatttcaa tcgccgtaaa cgcggcaata tcaaggccga acatatcagc
    19261 agcataaccg gactgaaaat cgaggttggc gttccaggtg gagtgagaaa ggttggtttt
    19321 gtatttatcg ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatagatacc
    19381 gccggttaag gttgaatcgt caatgaatcc ttcggcgcga gcctccggca ttgccataaa
    19441 gcccgacatt gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa
    19501 cgtacgcatg ggttaatcct ctttgacgta taaattgctg caccacaggt gaacagcgaa
    19561 aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg ctgactacag gaacagacta
    19621 tttttcgcga cgcgaattat caatctcttt ctgtaaccag aatgtaagaa catgagcaac
    19681 cgcacatatt ttgatgattt ttgttacaag gcttaagttt gcggttgaaa ggcttgcaga
    19741 agaataaaag cgtgaaattc cagcaagatg aaagcggtta catttta
//
LOCUS       DAZGXS010000083        19653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGXS010000083 DAZGXS010000000
VERSION     DAZGXS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..161
                     /locus_tag="ACHKG5_003111"
     CDS             <1..161
                     /locus_tag="ACHKG5_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869165.1"
                     /translation="ELFSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTRENYTY
                     NYDLLNAI"
     gene            complement(268..644)
                     /gene="rnpB"
                     /locus_tag="ACHKG5_003112"
     ncRNA           complement(268..644)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKG5_003112"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(677..1822)
                     /gene="garK"
                     /locus_tag="ACHKG5_003113"
     CDS             complement(677..1822)
                     /gene="garK"
                     /locus_tag="ACHKG5_003113"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0869166.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFRDIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1919..2809)
                     /gene="garR"
                     /locus_tag="ACHKG5_003114"
     CDS             complement(1919..2809)
                     /gene="garR"
                     /locus_tag="ACHKG5_003114"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0869167.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2839..3609)
                     /gene="garL"
                     /locus_tag="ACHKG5_003115"
     CDS             complement(2839..3609)
                     /gene="garL"
                     /locus_tag="ACHKG5_003115"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0869168.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3625..4959)
                     /gene="garP"
                     /locus_tag="ACHKG5_003116"
     CDS             complement(3625..4959)
                     /gene="garP"
                     /locus_tag="ACHKG5_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0869169.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5334..6905
                     /gene="garD"
                     /locus_tag="ACHKG5_003117"
     CDS             5334..6905
                     /gene="garD"
                     /locus_tag="ACHKG5_003117"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0869170.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7054..7389
                     /gene="prlF"
                     /locus_tag="ACHKG5_003118"
     CDS             7054..7389
                     /gene="prlF"
                     /locus_tag="ACHKG5_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0869171.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7389..7853
                     /gene="yhaV"
                     /locus_tag="ACHKG5_003119"
     CDS             7389..7853
                     /gene="yhaV"
                     /locus_tag="ACHKG5_003119"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0869172.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7908..8717)
                     /gene="agaR"
                     /locus_tag="ACHKG5_003120"
     CDS             complement(7908..8717)
                     /gene="agaR"
                     /locus_tag="ACHKG5_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0869173.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8966..10246
                     /gene="kbaZ"
                     /locus_tag="ACHKG5_003121"
     CDS             8966..10246
                     /gene="kbaZ"
                     /locus_tag="ACHKG5_003121"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0869174.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10269..10742
                     /gene="agaV"
                     /locus_tag="ACHKG5_003122"
     CDS             10269..10742
                     /gene="agaV"
                     /locus_tag="ACHKG5_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0869175.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            10753..11532
                     /gene="agaW"
                     /locus_tag="ACHKG5_003123"
     CDS             10753..11532
                     /gene="agaW"
                     /locus_tag="ACHKG5_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0869176.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11522..12400
                     /gene="agaE"
                     /locus_tag="ACHKG5_003124"
     CDS             11522..12400
                     /gene="agaE"
                     /locus_tag="ACHKG5_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0869177.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12418..12852
                     /gene="agaF"
                     /locus_tag="ACHKG5_003125"
     CDS             12418..12852
                     /gene="agaF"
                     /locus_tag="ACHKG5_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0869178.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12849..13982
                     /gene="nagA"
                     /locus_tag="ACHKG5_003126"
     CDS             12849..13982
                     /gene="nagA"
                     /locus_tag="ACHKG5_003126"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0869179.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14333..15487
                     /gene="agaS"
                     /locus_tag="ACHKG5_003127"
     CDS             14333..15487
                     /gene="agaS"
                     /locus_tag="ACHKG5_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0869180.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15500..16360
                     /gene="kbaY"
                     /locus_tag="ACHKG5_003128"
     CDS             15500..16360
                     /gene="kbaY"
                     /locus_tag="ACHKG5_003128"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0869181.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16527..17003
                     /gene="agaB"
                     /locus_tag="ACHKG5_003129"
     CDS             16527..17003
                     /gene="agaB"
                     /locus_tag="ACHKG5_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0869182.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITPETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17075..17845
                     /gene="agaC"
                     /locus_tag="ACHKG5_003130"
     CDS             17075..17845
                     /gene="agaC"
                     /locus_tag="ACHKG5_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0869183.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17835..18626
                     /gene="agaD"
                     /locus_tag="ACHKG5_003131"
     CDS             17835..18626
                     /gene="agaD"
                     /locus_tag="ACHKG5_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0869184.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18627..19382
                     /gene="agaI"
                     /locus_tag="ACHKG5_003132"
                     /pseudo
     CDS             18627..19382
                     /gene="agaI"
                     /locus_tag="ACHKG5_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         4796 a         5084 c         4978 g         4795 t
ORIGIN      
        1 aagagttatt ttcagataac accctcgaga aagacgaaaa ttctccggac aatggttttg
       61 aggtcgggga atataacaca tatgaagcat ataactcaga gaatcaatat tttaccagag
      121 agaactatac gtataattac gaccttttaa atgcaatata gacttaataa cttcttacaa
      181 ggtctattat taataccgta gatatttatt catcttaccc tgtgcaccaa aagcaaaaac
      241 ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg ggttctgtcg
      301 tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc
      361 agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc
      421 cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc
      481 ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc
      541 aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc
      601 cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag
      661 ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc gcggcgatat
      721 tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta
      781 tgctggtcaa tacgctgaag accgcatcaa tgccatgctg atgtacaacg ccaacatcat
      841 cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcga
      901 caccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca
      961 ccagcgtaca atcgtgaata tgttcctcca gattcagcgc cgtagtgacg atttcaatac
     1021 cacttttcag ttccgcacca agaaacgcca ttagcgccgc gcccatacca cccgcagctc
     1081 ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgacc tcggcatagt
     1141 gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa
     1201 agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa
     1261 tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa
     1321 gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg
     1381 cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg ataataatgt
     1441 ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca
     1501 cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa
     1561 taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg
     1621 gccctgtaac ccaggcgtga cgttcggaac cctgggtggc tgcaatcatc gcttccaccg
     1681 ttccttcgcc accgtcggca acaggaacag aaacgtactg tgcatcagga aaaatgtccc
     1741 gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt
     1801 ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc
     1861 tcttattcgg cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt
     1921 aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatccg
     1981 ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca gctgtgagcg
     2041 gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc ttaatatgca
     2101 gatcaatacg gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca
     2161 gcacggtact gcccgccagt ccaccgcgaa ttgcctgata aaccaggtcc gggttaacgc
     2221 ccgctttagt tgccagcgtt aacgcttctg acatcgcggc aatattcagc gccacaatga
     2281 cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc accacggaac
     2341 ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcctacca
     2401 tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca
     2461 tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag
     2521 aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca
     2581 gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt
     2641 cagcgatcgc tttagccgta gaagctgttt ctgcacctgc agcaatcacg tcagcaatag
     2701 cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca
     2761 ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct
     2821 ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag tggcagagcg
     2881 gaagacgccg agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg
     2941 cgcatcggct tcgaccggcg cgaggatacc gctgggtttg ccgtgcgcgc tggcacggtt
     3001 aaaaatgtgc tgaattgctt tttgtacatc cgggtgtgat gcattgccga gatggcctaa
     3061 tgccgcggcc agatcgctgg ggccgacgaa gatgccgtct acgccttcgg tagcggcaat
     3121 agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag tgatgttctt
     3181 gttcgactga gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga
     3241 gacgccgcga atgccttctg gcggataacg cgtcgatgcc accgcctgct ctgcttcctc
     3301 ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat
     3361 aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc ctttcagggc
     3421 cattaactgc ggaataaacg ttgaaatatc gtttggcgca tgttcgccat ccagcaccag
     3481 ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca
     3541 gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatc
     3601 gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac
     3661 tacgaagagg tagcagacca tcgccattag cgctgaacat cccacgaaaa ccagtgctgc
     3721 attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc
     3781 aacattgcca aagacgttaa agacgccgcc gcagaggcca acaatctctt taggcgcggt
     3841 gtcagaaatc accggccagc ccagcgcacc aaatccttta ccaaagaaag ccagcgccat
     3901 cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag
     3961 caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat
     4021 cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgctggaat
     4081 cgaggcgacc agacccactt tcagaatcga catacctttt tcctgcacca gataaatcgg
     4141 gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc
     4201 caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggtc cgcttgctgc
     4261 cgcactgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt
     4321 cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaatt tgatccacag
     4381 cgccgtcagc acaaaaccaa tcacccccat aacggtaaag acgtgctccc agccccaggc
     4441 gaaagtcagc cagccaagca gaggcgaaaa gagcgccagc gagaaatatt gcgccgagtt
     4501 aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc
     4561 gttcgccggg aatgatggcg cttccgagaa gccgagcata aagcgcataa agaacatgga
     4621 gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg tgaatagcga
     4681 ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcagccagcc
     4741 gccggggatt tgcatcagca agtaggccca gccaaaagca gaaaagatgt aacccatcga
     4801 aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg
     4861 atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg
     4921 cacgcctttc tttttttcgt caacggtgtc cagaatcatt ttatttacct cgggtactta
     4981 tgctgatttt tattattatg gggaagatgt tatttatgag tttcatttat gccgtaacga
     5041 caatgaactc gggaattagt ataagcagca cgagaataat aatcattgtg caaatgctaa
     5101 tttaattaat actatttaaa tattattttg agcatatgca cataaggttg cgcgctaaag
     5161 cacagatttg cgctttacct tgccgggcgg cattgcaatc cctgaaatga ttgacattga
     5221 tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt catttatttt
     5281 taaatgagac caggtcctca ttttaataac ctctggctgg agaatattgc acaatggcca
     5341 acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata
     5401 atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc
     5461 tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctcgac attccggcta
     5521 atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg
     5581 gaagctggat cgacgaatca atggttgtac tgccgaaagc gccgccgtta cacacgctgc
     5641 cactggcaac caaagtcccg gaacccttac cgccgctgga aggatacacc tttgagggct
     5701 atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc
     5761 actgtgtggc aggcgtggtg gactacgtag taaaaatcat tgaacgcgat ctgctaccga
     5821 aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggttgt ggcgtggcga
     5881 ttaacgcacc ggcggcagtt gtacctatcc gtaccattca caatatttcg ctgaatccta
     5941 actttggcgg cgaagtaatg gtgattggcc tgggttgtga aaagttgcag cctgagcgcc
     6001 tgctgactgg aacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt
     6061 tgcaggatga aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg
     6121 aacgccatct acaaaaactg aatcaacgtc agcgagaaac ctgtccggct tcagaactgg
     6181 ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg cgtaacggca aacccggcgg
     6241 ttgggtatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttc tcagaagtca
     6301 cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca
     6361 aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc
     6421 gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaatgta gtggagaaag
     6481 cgctcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg tcgcccggtc
     6541 aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg
     6601 gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc
     6661 cgtacggcct gatggcggta cccgtcatta aaatggcgac ccgcaccgag ctggcgaacc
     6721 gctggtttga tttaatggat attaacgcgg gcactatcgc caccggcgaa gaaaccattg
     6781 aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa
     6841 ccttctcgga tcaatgggga ctgcataacc agctggcggt gtttaaccca gcaccggtga
     6901 cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt ttttattgtt
     6961 taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc ttataatttg
     7021 agcagaaaac aggctgtaaa aggacagtga atcatgcccg ctaatgctcg ctctcacgct
     7081 gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc cgcgccagtg
     7141 cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat tctgcctggt
     7201 gggcaagtat ttatgtgccg actgggagat gagcaggagg atcatactat gaatgcattt
     7261 ttgcgttttc tggatacaga tatccagaac aacccgcaaa aaactcgtcc attcaacatt
     7321 caacaaggaa agaaacttgt cgctggcatg gacgtcaaca ttgatgatga gattggcgac
     7381 gacgaataat ggattttcca caaagggtta atggttgggc gctatatgct catccctgtt
     7441 ttcaggaaac ctacgacgct ttagttgccg aagtcgagac attaaaggga aaagatcctg
     7501 aaaattatca gagaaaagct gccacaaagt tattggcggt ggtccataaa gtgattgagg
     7561 agcatatcac ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg ttaggctctg
     7621 ggaaaaataa agactggtca cgggtaaaat ttggtgctgg tcgctatcgt ctcttctttc
     7681 gttacagtga aaaagagaaa gtcatcattc tgggatggat gaacgatgaa aacacgctgc
     7741 gcacctacgg taaaaaaaca gatgcctata ccgtattcag caaaatgtta aaaagaggac
     7801 atcctcctgc cgactgggaa accctcaccc gagagacaga agaaacccat tgatggtgtt
     7861 cacaatgccc gccgaacgca ttcgtgttga ccagcgggca ccctttctca ctccccgacc
     7921 agaatcactt caaccccagc ctttcgcagc ccttccaggc tatccgcagg aatgccttca
     7981 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     8041 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccggcggttt
     8101 aaacgggctt catcttcatt atgcgtgctg acgccgcgct ccagatcgat cgcatctaca
     8161 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     8221 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     8281 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtgcttg
     8341 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     8401 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcattgcggt gttcagcgcg
     8461 cttttatctt ccactgatgg ctcgactgac ggcgttgtgc tatcgcagat caacgcgcca
     8521 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcgtt gcggatcgtc
     8581 acggtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     8641 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     8701 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     8761 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattgacgc aagctactct
     8821 cgtttcagtg actttcatta tgtttctttt gtgaatcaga tcagaaaact attatctttc
     8881 gttttatttt tatctcacca tgacgcagta tcaactgaaa caaaacgaaa gattaatatc
     8941 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     9001 agcgggcaaa acaaatggaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     9061 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     9121 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     9181 tcagctcgcc gactcgttga atttcccgca ggatgcgttg attctgggtg gtgaccatct
     9241 ggggccaaac cgctggcaaa acctgccggc cgctcaggca atggccaatg ccgatgattt
     9301 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     9361 tcaggacgat ccgattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     9421 gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa gccgatctgg agtatgtcat
     9481 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     9541 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     9601 tttgaatgcc atctggccac gcatcattgc cctggtggtt caacccggcg tcgaattcga
     9661 tcacaccaac gttattgatt atcagcccgc caaagcgacc gccttaagcc agatggtcga
     9721 aagctacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatcgct
     9781 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     9841 cctgcgtgaa gctctgttct ctctggcagc gattgaagaa gaactggtgc cagcaaaagc
     9901 ctgttctggt ctgcgtcagg tgctggaaaa cgtgatgctc aaccgcccgg aatactggca
     9961 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttata gctactcgga
    10021 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggtacg
    10081 taatctggcg gattcaccaa ttccgctgcc gctgatcagc cagtatctgc cgctgcagta
    10141 cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg gaactcatta tcaaccatat
    10201 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
    10261 aacacgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
    10321 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtcgcc aacgatgagg
    10381 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
    10441 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
    10501 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
    10561 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaagcaa caaatcgcca
    10621 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
    10681 tggaatgctt cgttcagggc atcccgacag agcctgctgt ggacctcttt aaattacttt
    10741 gagggattca tcatggaaat cagtctgttg caggcattcg cgttgggcat tatcgccttt
    10801 atcgctggcc tggatatgtt taacggctta acccatatgc accgcccagt ggtcctcggc
    10861 ccgttagtcg ggctggtact tggcgatctg cataccggta ttttaaccgg cggtacgctg
    10921 gaactggtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa tgtgattatc
    10981 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcagtc
    11041 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
    11101 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
    11161 gaacgcgtaa actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    11221 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    11281 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    11341 ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca tcctgggctt tgttgccgca
    11401 gcctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    11461 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    11521 gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa aacgaagaaa cattgctgac
    11581 tggcgtcaat gaaaacgtgt atgaagatca gagcattggc gcggagctga cgaaaaaaga
    11641 tatcaatcgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    11701 gcaggcttcc ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    11761 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    11821 ggtgacattt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    11881 tattcagagc accaaaattg ccgtcggtgc gccgctcggc gggattggcg atgcaatgtt
    11941 ctggctaacg ctactgccga tttgtggcgg gattggagcc agtctggcat tgcaaggctc
    12001 cattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtccacctgg ggctgcgttt
    12061 tggtctggcg cattatgctt accgcatggg cgtggcggcg attccactca ttaaagctaa
    12121 taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg acggtaatcg gtgcgctggt
    12181 ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca gcgggcgacg cagtggttaa
    12241 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgttgg tctacaccct
    12301 gaccatgttt tggttggtac gccgcggctg gagtccgctg cgtctgattg cagttaccgt
    12361 ggttctcggc atcgttggta aattctgcca tttcctttaa atacaatgag gtattagatg
    12421 ttaagtatta ttttgacagg gcatggcggg tttgccagcg gcatggaaaa agcaatgaag
    12481 caaattctcg gtgagcaatc gcagtttatc gccatcgatt ttccggaaac ctcaagcacc
    12541 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    12601 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    12661 caaaaaccgg gctgtgaagt gatcaccggc accaatttgc aactgctact ggagatggtg
    12721 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacat
    12781 cgtgggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    12841 gaagggatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggctcg
    12901 atgaccatca gttgcgtatt gctgacggtg tcatcgcagc aatcgaaccg attccagtga
    12961 gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg
    13021 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    13081 acaaggcacg cgaaggtgtc ggcagttggt tgccgactac cgtaaccgcg ccgcttagta
    13141 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    13201 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    13261 cgccggagtt gtttcgcgag cttgaaattg ccgagctgga tcaattgatt gccgtttctc
    13321 agcacacctt acgcgtggta gcgctggcac cggaaaaaga gggggcattg caggccatcc
    13381 gccatcttaa acagcaaaat gtacgagtga tgctggggca tagcgcggcg acctggcaac
    13441 aaactcgcgc cgcgtttgat gctggtgccg acggcctggt gcattgctat aacgggatga
    13501 caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac aagcgcgcct
    13561 ggctggaact gatagccgat ggtcatcatg tgcatccggc ggcaatgtcg ctgtgttgtt
    13621 gctgtgcgaa agagagaatc gtactgatca ccgacgcgat gcaggcagcc gggatgccgg
    13681 atggtcgcta tacgttatgt ggcgaagaag tgcagatgca cggtggcgtt gtccgtaccg
    13741 cgtccggtgg gctggcgggc agtacgctgt ctgttgatgc ggcagtgcgc aacatggtcg
    13801 agttgacggg cgtaacgcct gcggaagcca ttcatatggc gtcgctgcat ccggcgcgaa
    13861 tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg caaacgcgcc agcgtcgttg
    13921 cgctggatag cgggctgcat gtgcaacaaa tctggattca gggtcaatta gcttcgtttt
    13981 gatggtttgc tccattattg ggccttcact tcccccgtaa ggcctttctt tttctttcgt
    14041 tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt cttatccgac ctacagttgg
    14101 tgaccgcaag gccggataaa gcgtttgcgc cgcatccagc aatccctttt gcttccttta
    14161 tcttttcttt caacgatcac ggatttcgtt ttatttcctt tttctccatt gaactttcgg
    14221 tttcttttct atagatttta atcaacgaaa gacatcacca agtgaaatga aacgaaaggc
    14281 aagtgaaagc gacaacgccc gacgtcaagt tcatcagact aaggattgag ttatgccaga
    14341 aaattacacc cctgctgccg ccgcaaccgg tacatggact gaagaagaga tccgccatca
    14401 gcctcgcgca tggatccgtt cgctcaccaa catcgacgcg ctacattccg cgcttaataa
    14461 cttccttgaa ccgttactgc gcaaagagaa tctgcggatc atcctgaccg gagccggaac
    14521 ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt
    14581 cagcgccgta ccgaccaccg atctggtcac taatccgatg gactacctga acccagccca
    14641 tccgctgctg ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg tcgcagccgt
    14701 ggaactggca aatcaatttg taccggaatg ctatcacctg ccgatcacct gcaacgaagc
    14761 gggcgctctt taccaaaacg cgatcaacag cgataacgcg tttgccgtgc tgatgcccgc
    14821 agaaacgcac gatcgcggct ttgcgatgac cagcagcatt accaccatga tggccagctg
    14881 cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg tggcggatcg
    14941 ttgccaggcg atcctgacct cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc
    15001 gtggaaacgg atcgtttatc tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc
    15061 gctgaaagtg ctggaactga cggcgggtaa actggcggcc ttttatgatt ctccaaccgg
    15121 attccgtcat ggaccaaaat cgctggtcga taacgaaaca ctggtggtgg tatttgtctc
    15181 cagccaccct tacacccgtc agtatgatct tgatctgctg gctgaacttc accgtgacaa
    15241 ccaggcaatg cgtgtaatcg ccatcgccgc ggaaagcagc gacatcgtcg ctgccggtcc
    15301 acatatcatc ctgccaccgt cacgtcactt tatcgacgtt gagcaggcat tttgcttcct
    15361 gatgtacgcc cagacgtttg cactgatgca gtcgctgcac atgggcaata cgccggatac
    15421 cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca
    15481 ggcataagag gatcgcatta tgagcattat ctccaccaaa tatctgttac aggacgccca
    15541 ggccaatggc tacgcggtgc ctgcttttaa cattcataac gccgagacga tccaggcgat
    15601 cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc gccggaacgc cggggacctt
    15661 taaacatatc gcgctggaag agatctacgc cctgtgcagc gcctattcca caacctacaa
    15721 catgccactg gcgctgcatc tcgaccacca cgaatcgctg gatgatattc gccgtaaagt
    15781 ccacgcaggt gtgcgcagtg cgatgatcga cggcagccac ttcccgtttg ccgagaacgt
    15841 aaagctggtg aaatcggttg tcgacttctg ccactcccag gattgcagcg tggaagcaga
    15901 actgggccgc ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa gtgcattcct
    15961 gaccgatcca caagaagcta aacgctttgt cgaactgact ggcgtcgaca gcttggcggt
    16021 agcgattggt acggcgcacg gcttatacag caaaacgccg aagattgatt tccagcggct
    16081 ggcggaaatt cgtgaagtgg tggatgttcc tctggtgcta catggtgcca gcgatgttcc
    16141 ggatgaattt gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg ttgccacaga
    16201 attaaaaata gccttcgccg gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa
    16261 tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa
    16321 aattaatgtc tgtggttcag cgaatcgaat ttcagcataa tcattagttt tttatgattt
    16381 atcccaatgt acttcccgat taatatcggg gagtgcctta atggaaaagg agatgactaa
    16441 accttaataa atagatcagt acaatatcgc aacaataata tatttaaaaa aattatatta
    16501 ttcaacttta tggtgaggat tacacaatga gcagtccaaa tattctctta acccgtattg
    16561 ataaccgtct ggttcacggt caggttggcg tgacctggac atccaccatc ggtgcgaatc
    16621 tgctggtagt cgtggatgat gttgtcgcta acgatgatat tcaacagaaa ttaatgggta
    16681 ttaccccaga aacctacggc tttggcattc gtttctttac tatcgaaaaa accattaacg
    16741 tcatcggcaa agctgcaccg catcagaaga tcttcctgat ttgccgtacg ccacaaacgg
    16801 tacgtaaatt ggtagaaggt ggcattgacc tgaaagatgt caacgtcggc aatatgcatt
    16861 tctcggaagg gaaaaagcaa atcagcagta aagtttatgt cgatgaccag gatctcacgg
    16921 acttacgttt tattaaacaa cgtggcgtga atgttttcat tcaggacgtc cctggcgatc
    16981 aaaaagaaca aatccctgac taaatctaaa atcgccttaa tattggtttg aggtaataaa
    17041 aatgcatgaa ataaccctac tttagggatt atccctggcg gcgttagttt tcgttctggg
    17101 gattgatttt tggctggaag ccttattttt attccgcccg ataatcgttt gtaccctaac
    17161 tggcgctatt ctcggtgata ttcagactgg cttaattacc ggtggtctga cagagttggc
    17221 tttcgccgga ttaacccctg caggtggtgt tcagccgccc aacccgatta tggcgggtct
    17281 gatgaccacc gtcattgcat ggtctacggg cgttgatgcc aaaacggcaa ttggtcttgg
    17341 cctgccgttt agtttgttaa tgcagtacgt cattctgttc ttctattctg ctttctcatt
    17401 atttatgacc aaagccgata aatgcgcgaa agaggcggat acggcagcgt tttcccgact
    17461 taactggaca acgatgctca tcgtcgcttc agcgtatgcg gtgattgctt tcctctgtac
    17521 ttacctggca caaggggcga tgcaggcgct ggtgaaagcg atgcccgctt ggctgaccca
    17581 cggctttgaa gtggctggcg gtattctgcc tgccgttggt tttggcttgc tgctgcgcgt
    17641 gatgttcaaa gcacaatata tcccttacct gatcgccggc ttcctgtttg tttgctacat
    17701 ccaggtcagc aacctgttgc cggttgccgt actgggcgca ggctttgcgg tgtatgagtt
    17761 tttcaatgcg aaatcccggc agcaagcgca accgcagccc gttgccagta aaaatgaaga
    17821 agaggactac agcaatggga tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc
    17881 gttcgtcgct gctgcaagcg agctttaact acgaaaggat gcaagcgggc ggttttacct
    17941 gggcgatgtt gccgatcctg aaaaagattt ataaggatga caaaccgggc ctaagcgcag
    18001 cgatgaaaga taacctcgaa tttattaata cccacccgaa tctggtcgga ttcctgatgg
    18061 ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga caccattaaa ggcctcaaag
    18121 tggcactgtt tggcccaatc gccgggattg gcgatgcgat tttctggttt actttgttgc
    18181 cgattatggc gggaatttgc tcatcatttg ccagccaggg aaacctgctg gggccgattc
    18241 tatttttcgc cgtttacctg cttatctttt tcctgcgcgt cggctggacc cacgtcggtt
    18301 attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa ctcgcagatg attgctcgct
    18361 cggcaaccat cctcgggatc acggtaatcg gcgggctgat cgcttcgtat gtgcatatta
    18421 acgtggtgac atcgtttgcc atcgacagta cccacagcgt cgcgctgcaa caggatttct
    18481 tcgataaagt cttcccgaac attttaccga tggcctacac cctgctgatg tattacttcc
    18541 tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt gacttttgtg ctctctattg
    18601 tttgttccgc attcggcatt ttgtaaatgg aacgaggcac tgcgtctggc ggtgcctctt
    18661 tacttaagga atttcatcct gtgcaaaccc ttcagcaagt tgaaaactat acggcgttaa
    18721 gtgaacgtgc cagcgaatat ttattggccg tgatccgtag caaaccggat gccgtgattt
    18781 gcctggcgac cggagccacg ccattactga cgtatcatta tctggtagaa aaaatccact
    18841 agcagcaggt tgatgtcagc cagctcacct tcgtgaagct cgacgaatgg gtggatctgc
    18901 cattaacgat gcctggtacc tgcgaaactt tcctgcaaca gcatatcgtg cagccgctgg
    18961 ggctacgtga agaccagctc atcagctttc gctccgaaga gataaatgag acagagtgcg
    19021 aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga tttatgcgtt ctcggattgg
    19081 ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaaa cctgcaaccg gcctgccata
    19141 tcagtcaact tgatgccaga acacagcaac atgagatgtt aaaaaccgcg ggtcgccccg
    19201 tgactcgtgg gatcacctta ggcctgaagg atattctcaa tgcccgcgag gttttgttac
    19261 tggtgactgg cgaaggaaag caggatgcga cagaacgttt tctcacggct aaagtctcta
    19321 cctctatccc ggcttcgttt ttgtggctgc acagtaactt tatttgttta attaatacct
    19381 aaccagatga aaaatttgct caaaatgttt aaattaactt atgcaacagt cacgcattat
    19441 attaaataac atttgactgg gttgaacata acgccaatag caaaaggagt tatgttcaac
    19501 aatttaattt tagatataca tataacttgt tctattaata aaccatctcg tattctgact
    19561 cgccccctgg cgcagcaaag tcatgcactt atactctttt gaatacatag aaaattgata
    19621 tcaatataat gaaaatatca aatatttgca tat
//
LOCUS       DAZGXS010000084        19606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGXS010000084 DAZGXS010000000
VERSION     DAZGXS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..242
                     /gene="yahH"
                     /locus_tag="ACHKG5_003133"
                     /pseudo
     CDS             1..242
                     /gene="yahH"
                     /locus_tag="ACHKG5_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            292..1242
                     /gene="yahI"
                     /locus_tag="ACHKG5_003134"
     CDS             292..1242
                     /gene="yahI"
                     /locus_tag="ACHKG5_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0869185.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1252..2634
                     /gene="yahJ"
                     /locus_tag="ACHKG5_003135"
     CDS             1252..2634
                     /gene="yahJ"
                     /locus_tag="ACHKG5_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0869186.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2933..3343
                     /locus_tag="ACHKG5_003136"
     CDS             2933..3343
                     /locus_tag="ACHKG5_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0869187.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3597..4580
                     /locus_tag="ACHKG5_003137"
     CDS             3597..4580
                     /locus_tag="ACHKG5_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0869188.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4629..6113
                     /locus_tag="ACHKG5_003138"
     CDS             4629..6113
                     /locus_tag="ACHKG5_003138"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0869189.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSPPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6106..7077
                     /locus_tag="ACHKG5_003139"
     CDS             6106..7077
                     /locus_tag="ACHKG5_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0869190.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7074..8030
                     /locus_tag="ACHKG5_003140"
     CDS             7074..8030
                     /locus_tag="ACHKG5_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0869191.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8117..9166
                     /gene="yahK"
                     /locus_tag="ACHKG5_003141"
     CDS             8117..9166
                     /gene="yahK"
                     /locus_tag="ACHKG5_003141"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0869192.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9409..10224
                     /gene="yahL"
                     /locus_tag="ACHKG5_003142"
     CDS             9409..10224
                     /gene="yahL"
                     /locus_tag="ACHKG5_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP0869193.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10676..10885
                     /locus_tag="ACHKG5_003143"
     CDS             10676..10885
                     /locus_tag="ACHKG5_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869194.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10902..11573)
                     /gene="yahN"
                     /locus_tag="ACHKG5_003144"
     CDS             complement(10902..11573)
                     /gene="yahN"
                     /locus_tag="ACHKG5_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0869195.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11720..11995
                     /gene="yahO"
                     /locus_tag="ACHKG5_003145"
     CDS             11720..11995
                     /gene="yahO"
                     /locus_tag="ACHKG5_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0869196.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12093..13679)
                     /gene="prpR"
                     /locus_tag="ACHKG5_003146"
     CDS             complement(12093..13679)
                     /gene="prpR"
                     /locus_tag="ACHKG5_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0869197.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13918..14808
                     /gene="prpB"
                     /locus_tag="ACHKG5_003147"
     CDS             13918..14808
                     /gene="prpB"
                     /locus_tag="ACHKG5_003147"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0869198.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14964..16133
                     /gene="prpC"
                     /locus_tag="ACHKG5_003148"
     CDS             14964..16133
                     /gene="prpC"
                     /locus_tag="ACHKG5_003148"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0869199.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16167..17618
                     /gene="prpD"
                     /locus_tag="ACHKG5_003149"
     CDS             16167..17618
                     /gene="prpD"
                     /locus_tag="ACHKG5_003149"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0869200.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17658..19544
                     /gene="prpE"
                     /locus_tag="ACHKG5_003150"
     CDS             17658..19544
                     /gene="prpE"
                     /locus_tag="ACHKG5_003150"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0869201.1"
                     /translation="MSFSEFYQRSINEAEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         4756 a         4797 c         5315 g         4738 t
ORIGIN      
        1 tccggcctac gaatggcgca agaatctgta ggcctgataa gcgtagcgca tcaggcattt
       61 gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc tacgaatggc acgagaattg
      121 taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc ggcgtgaacg
      181 ccttatccgg cctacgaatg gcacgagaat ttgtaggcct gataagcgta gcgcatcagg
      241 cagtctggcg ttggtcataa ccccatcacc ctctgtagcg gacataacaa catgaaagaa
      301 cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag ccagtcgatt
      361 gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat gctggcttcc
      421 gattacgaca ttgtgctgac ccacggtaac gggccgcagg tcgggctgga tttacgccgt
      481 gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa ctgtgtggca
      541 gatacgcagg gcggcattgg ctacctgatc caacaggcgc tgaacaaccg gctggcgcgt
      601 cacggcgaga aaaaagccgt caccgtggtg actcaggtgg aagtggataa aaacgatcca
      661 ggttttgccc atcccaccaa gcccattggt gcattcttca gtgaaagcca gcgtgacgaa
      721 ttacaaaagg caaaccctga ctggcgtttt gttgaagatg ctggacgggg ctatcgccgc
      781 gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ccgccattaa ggcgctgatc
      841 caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa ttcctgtggt gcgtactgac
      901 gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctctac cgcgctgctg
      961 gcccgtgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa agtctgtatc
     1021 cactttggta aacccgagaa gcaggcgctg gatcgggtgg atattgccac catgactcgc
     1081 tatatgcagg aaggacattt cccgcccggc agcatgttgc caaaaatcat cgccagcctg
     1141 acgttcctgg aacagggcgg taaagaagtg attatcacca cgccggaatg cctgccagcg
     1201 gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt aaggacgtaa gatgaaagaa
     1261 aataatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc cgccgcgctg
     1321 tttggtacct ctgtgccgct cgcccatgcg gcggtctctg gcaccacaaa ctgcgaagcg
     1381 aacaacacca tgaaaatcac tgacccgcat tactatctcg acaacgtgct gctggaaacc
     1441 ggttttgact acgaaaatgg cgtggcggtg cagacccgca cggcgcgcca gaccgtggag
     1501 attcagaacg gcaaaattgt cgcgctgcgc gagaataagc agcacccgga tgccacgctg
     1561 ccgcactatg acgctggcga taagctgatg ctgcccacca cccgcgacat gcatattcat
     1621 ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca atcgcccggc aggcaccacc
     1681 atccaggaca tgatcaaact cgagcagaaa atgctgccgg agctgcaacc gtacactcag
     1741 gagcgggcag aaaaactgat tgatttattg cagtcgaaag gcaccaccat tgcccgcagc
     1801 cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg
     1861 gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct tcccgcagca cggtttgctg
     1921 ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg cgggtgcgca ttacgtcggc
     1981 gggctggacc cgaccagtgt cgatggcgcg atggaaaaat ccctcgacac catgttccag
     2041 attgcgctgg actacgacaa aggcgtcgat attcacctgc acgaaaccac tccgtcgggc
     2101 gtggcagcca tcaattatat ggttgaaacg gtagagaaaa cgcctcaact gaaaggtaag
     2161 ctgaccatca gccacgcctt tgcgctggct acgctcaacg agcaacaggt agatgaactg
     2221 gcgcatcgca tggcggcgca gcaaatttct atcgcctcga cggtgccgat tggcacgctg
     2281 catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag tgatgactgg cactgacagc
     2341 gttatcgacc actggtcgcc ttatggtctg ggcgacatgc tggaaaaagc caatctctac
     2401 gcgcagctct atattcgtcc taacgaacag aacctctccc gttcgctgtt tctagccact
     2461 ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat ggccaaaagc gcaggatgac
     2521 gccagctttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg tatctcgccg
     2581 agaaccgcaa cgttccataa agggcaactg gtgtggggga gtgtggcagg ttgatgcgca
     2641 tttgaaacgc ctgcaacggt gaatagtaag agatttaagc cccagggagt ggggcgggaa
     2701 tggtttgcta atgaatgatc ctgaacttta taatctcccg gcataatttc caggggatta
     2761 tttgagagaa atacacaaaa gtgagtttta acaaaggtat ttatcaggcc ataaatgtga
     2821 cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat gatactcttg ctgatggtta
     2881 ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc aaaaatattg ccatgaccat
     2941 aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt tgtacttttg gcgattcagc
     3001 cgcgttggcc gatcacctcg caacgttgat tgcgactggt gttaaaacag cttcctgtgg
     3061 ttcgctggcg ggatgtattg aagacaacgc atttccgatg attggtgagt ataagattgt
     3121 agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg attggcctgc atttactgcg
     3181 cttttctgat gtcaccgcgg agcttgcccg taaggaaggt gaaggtgacc tcagtcttga
     3241 atattggcgt aatgaacacc gtcgtttttt ccaggcagaa ggaagctatt caccagaaat
     3301 ggacgttatc tttgaagaat atgcccttat tgacgttgtg tagataacta tttatctaca
     3361 tgaaataagc tcacaaacat aaaatatcgc taattttgtt tattttgctg ttttttacgt
     3421 gatgaatgtc acacttatta ttgctgcccg ttttgtccat atttataacc atatttttaa
     3481 tagctgcctg aaatagagct gtttattctg cataaaaata ttcgaaaatt cttattctct
     3541 ccagcgcgct aatttgggcg aacacttcct gactaccctg caatgaggct gaaatgatga
     3601 ataaacgttt tgttatcaat atggtgtctt cgctgttatt aggcgctgcg ctaatttctg
     3661 cgccgctcca ggcagcggaa aaagtcgtgg tgaatatttc taaagtcgat ggtatgccgt
     3721 ggtttaatcg catgggcgaa ggtgtggttg aggcgggtaa agcctttggt gttaatgcct
     3781 cacaggtggg gccttcaagt actgatgctc cgcaacaagt taaaattatt gaggatttaa
     3841 tcgcccgtaa ggttaatgca attactatcg tgccaaacga tgccaacgtg ctggaaccgg
     3901 tattcaaaaa agcgcgagat gccggaattg tggttctgac caacgaatcc cccggtcagc
     3961 caagtgctaa ctgggatatc gaaatcattg ataacgaaaa atttgccgct gaatatgttg
     4021 agcatatggc gaaacgcatg ggtggcaagg gcggctatgt gatttacgtg ggtagtttga
     4081 ccgtgccgca gcataactta tgggctgact tgctggtgaa ataccagaaa gagcattacc
     4141 cggatatgca tgaagttacc cgccgcatgc cggttgccga gagtgtggat gtctcacgtc
     4201 gtactacact ggacctgatg aaaacctatc ctgacttgaa agcggttgtc tcctttggtt
     4261 caaacggtcc gattggcgcg ggacgtgcgg tgaaagagaa acgggcgaaa aataaagtcg
     4321 cagtctatgg catgatgatc ccttcacagg cggcatcact gattaaaagt ggtgatatca
     4381 ccgaagggat tacttacgat ccggcctctg caggttacgc actcgcagcc gtcgccagca
     4441 cgttgctgaa gggtgaagag atcaagcctg gattagaaat gcagaatctg ggtaaggccg
     4501 acgtagatat ggataagcga attatccgtt tccataaggt attactggtc aacaaagaca
     4561 atatcgactc actttattaa tccatttctt cggggcgaaa gccccgattc ttattgccag
     4621 gggatgttat ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt catgccacgc
     4681 gagcgctgcg cgatgtttct ctcgacttta tgtcgggtga agtgcactgc cttgccgggc
     4741 aaaacggctg tggtaaatcg acgttgatta aaattatgtc cggtgtttat cgcccggatg
     4801 agggagcgga aattacgctt ggtgggaaaa actggtcaaa gctgacaccc gccgcttcgg
     4861 tggcgcaggg gattcaggtg atttatcagg acctctcttt atttcctaac ctgagtgtct
     4921 gggagaatat cgccgtgaat cactatcacc acggtctgtt tgttaaccgc cgtcgtctgc
     4981 gtgaggtggc gcaggcggca atgactagta ttaacgtcac attgccactg gatacgctcg
     5041 tttctgagct ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca ctggcgcagg
     5101 acgcacggtt gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag gaagtgcaag
     5161 ggttattaca ggtggtgcat caattgcgtg aacgcgggat ctgcgtggtc tttgtcagtc
     5221 atcgtctgga agaagtgatg gaagtttccg accgtatttc agtgctgaaa gatggtgagc
     5281 tggtcgggac ttttccggct gcagagatga ccacaaaaca actcggtttc ctgatgaccg
     5341 gccaggagtt tgaatatcag gtgcgagagt tgtggcaggg aaaatccagc cccccggtgc
     5401 tggaggtgcg taatttaagt cgtcatgggg aatatctaaa tattaacttg cgggtggaag
     5461 cgggcgaagt ggtatcgatt gttggcctgc ttggtgcggg gcgcacggaa ttatgtctga
     5521 gtctgtttgg tatgacccga ccagatgctg gcgagatcct catcaacggc cagctggtta
     5581 cactgcatag taaccaggac gccatccgcc acggtattgg ttatgtttcg gaagatcgca
     5641 tgtcgcgcgg tttggtgatg gcgcaatcta ttgaagacaa catcattagc actgtttttc
     5701 acaaggtaaa agatcgcttc ggctttctga gtgaaactaa agtgcgtgac ctggtggaca
     5761 ggctaatcaa ggcattgacc atcaaagtct ctgatcctca tcttccggtg aatacgcttt
     5821 ctggtggtaa cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg ccacgactgt
     5881 tgattctcga tagcccgaca gtcggtgttg atatcgccaa caaagcagga atatacggca
     5941 ttatcagcga tctggcggct catggcattg cggtattgat gatctgcgat gaaattgaag
     6001 aagcgtggta tcaaagccac cggattttgg taatgcaaaa aggtcagatc acccatagct
     6061 tcctgcctga cagcagttct caggccagga ttgcggaggt ggtaaatggc tgaactgaaa
     6121 aaacgccatg aattctggct ggcattactg attgtggtgc tgtttgttgg ccttgcctgg
     6181 cgcagcgacg agtttctgac attcggtaat ttgtacgatc tcgccaataa ctatgccatg
     6241 ttgactattc tcgcctgtgg tttgtttgtg gtgttgattt ccggtggaat tgatatttcg
     6301 tttccagcaa tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca aaaaattggt
     6361 ggcaacttcg ctgtcgcgtt tgcactggca ggctgcatcg gcattttact tggcttaatt
     6421 aacgccttac tggttaatcg cctacgggtg ccttctatca tcatcactat ctcgacgctg
     6481 aatattttct atggcctgct gttatggttg agtaaaggtg tgtggctgta cgactttccg
     6541 ccgtggtttg agcagggggt tatgttgttc aagtacaccg atgctgatgg ctatgactat
     6601 ggccttggtc tgccgctgat cgccatgatt acggtggtgc tgctgacagc gtttatcatg
     6661 aatttcacca gtgtagggcg caaaatttat gcccttggcg ggaaccgcga atcagccagt
     6721 cgcatcggct ttagcgtgct gaaactgcaa cttttcgtct acggctatat gggattgatg
     6781 tctggcgctg cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc cgattctctt
     6841 ctgggttatg agctgacagt actggctgcg gtggtgcttg gcggcactag tttgctcggc
     6901 gggcgcggca cgttaactgg tactttgctc ggcgtggtgt tgttggcagt gatgcaaaac
     6961 gggctaaatt tattgggagt ctcgtcttac tggcaaacat tgatcaccgg catcatcatc
     7021 gttgccagca ttagcgccac ggcgtggagt cagcatcaga accggagcct gctatgaaaa
     7081 aatcgtggcg taataacgtt gagttttact tgatcggcct gctggtgcta actgttgccg
     7141 cgttcagcat tactatgccg gagattttct ggtcgataag taatttccag tccgtcgctt
     7201 cgcaaatgcc cgtgctgggc attctggcgt tggctatggc ggtgaccatg ctttgcggtg
     7261 gcattaactt gtcaattata gccactgcta acgcctgttc actggtgatg gcgtgggtgg
     7321 cgactcagta tccaccgggt attgctacgg ttgtcgctac gttactggcg ggcgctggcg
     7381 cggcggtgat aatcggttta tgtaacggcg tattgattgc tggtattcga gtgttgccaa
     7441 ttcttgcaac gcttgggatg atgacgttgc tcaaagggat aaatattctg gtgacaggtg
     7501 gtagcgccat tgccaattat ccctcatggg tgctgtggtt gaatcatgcg caatggttcg
     7561 gtattccgct accgatgtgg ttgtttgccg cagtggcact ggggctgtgg atattgctgg
     7621 agaaaacgcc gctcggtaaa gccatttacc tgattgggtc taacgaacga gcaacacttt
     7681 actccggcat taacactcgt cgggtgctga tttgggttta tgtcatttcc gccttgcttt
     7741 gtgcggtagc ggcatttttg atgatgtcga aactcaactc ggctaaggcc tcttatggcg
     7801 aatcgtactt gctggtgtca atcctcgcgg cagtactcgg cggggttaat ccggacggcg
     7861 gcagcgggcg gataattggt atggtgctgg cgctgtttct tctgcaaatt attgaaagcg
     7921 gcttcaatat tctcggtata agcccctacc tgacgatggc cttatgggga acgttgctgc
     7981 tctgtttcat tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa atattggcta
     8041 tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata cacatagcta
     8101 atcaggagta aacacaatga agatcaaagc tgttggttca tattccgcta aacaaccact
     8161 tgaaccgatg gatatcaccc ggcgtgaacc gggaccgaat gatgtcaaaa tcgaaatcgc
     8221 ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg cggggacggt
     8281 ttacccctgc gtgccgggtc atgaaattgt ggggcgtgtg gtagccgttg gtgatcaggt
     8341 agaaaaacat gcgccgggcg atttggtcgg tgtcggctgc attgtcgaca gttgtaaaca
     8401 ttgcgaagag tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg gcacctataa
     8461 ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac agatcgtcgt
     8521 tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg cggtggctcc
     8581 tttgttgtgt gcagggatca ccacgtattc gccgctacgt cactggcagg ccgggccggg
     8641 taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta agctggccca
     8701 cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg aagcggcaaa
     8761 agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg cggctcatct
     8821 gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg acgattttac
     8881 caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga caccgcataa
     8941 atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccggtt ctatgattgg
     9001 cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca tcgtggctga
     9061 tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc tgcgtggtga
     9121 tgtgaaatat cgttttgtta tcgataatcg cacactaaca gactgaaaaa attaataaat
     9181 acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta aggagagcga
     9241 aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa attaactccc
     9301 tccagtacct aatacttcac acgttagtta tgagcgattt ctgatagtgc ctggtttaat
     9361 cagagcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat gatatcatta
     9421 aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa tacggttaaa
     9481 aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga tatattcaag
     9541 gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt agtcgatgac
     9601 gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga cgataacgca
     9661 ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat ggattaccct
     9721 gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac agtttgggta
     9781 tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg tgcaggctgg acgctttacc
     9841 gggcttaaga aattagtgca gtttatcaat tcagtgcgaa cagattgcaa atgtattctt
     9901 cttgaacacc atatgccttt gctgagactt ttccctaaag ggaaagagtg tatgcatgtg
     9961 gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat taagcaggcg
    10021 cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc tgaatcactg
    10081 gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc acgattaaat
    10141 gaagggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt aaataagcac
    10201 gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt gttattccac
    10261 ctgagagtga atcgcaatgc aggtaatcat ttcatctgta tatctgtatg ccaactggca
    10321 ttccatctct tttttcgccg atattttgtt gtgatcggcg acacttcgct taaaaaagca
    10381 ccagtagtgg tttcgcagcc atgcggtgta taaaaaaaaa tgatctcatg cagatgtttt
    10441 gtgaatgtgt gggttgacat tcatatgaaa aaaatcataa ttccatcatg tttgtgtggg
    10501 gtcttttctg tatcttacgc atcgcactca agcctgacag aaaatatgct gtaaggctca
    10561 tatcaaaaac cgccattagc tcatcaggaa gagcagacga caaccaggat tgttgtatgg
    10621 tacggggttc gaggccccga tggcggtcca acttttcaaa actcttctga attagatccc
    10681 cttattatca tccctgcaaa gtggaacatt gccattgttt ggctattcag gacgggtcag
    10741 accaatgaaa aaagcccaca caggggagag tgggctgaaa tgggaagcta aagactcaag
    10801 taaacttatc ggaaataagg accacgcatt acgggggcta tcatcgccga tggtcgggat
    10861 tcggcaaatc aggttgatta cgtagagagt aaattatctg ctcaccgctg cgtcacccct
    10921 tcgtaaatca ggcgtagcgc gaatacacca ataattgcac caataacccg actggcaacg
    10981 cgttgcatac gcccataagc acgacgcaca gcgggcaaag aaaacgcctg actaagaaaa
    11041 actcgccaga taattgatgc gagcacaatc cccgcccagg ccattaaacg tgcccatgtt
    11101 ggtgtttcgg catttaatgt tactgagaaa atactaataa aaaataaaac ggtttgtggg
    11161 ttagagagat cggtaattaa tccacggcga aaaaagacat accagggggc gttaatcggt
    11221 tgttgtagtg tgctcatttg cggtgttgac tggcggcgca tgctgcacca cgcaaaccat
    11281 aagagataag cgccgccgac gattctgata agcgaaaaaa tctcctcaca ctgcgtaatt
    11341 agcgttgcaa gaccaaacaa acccaacccg gaataaaatg catcgcccag cgccacgccc
    11401 agtccggtca gcactcctgc gcgtcgaccg gaagccaggc tggtttgtac taccacaaaa
    11461 agattggctc ccggattaaa aaaagtaatc acgaacagtc ctacggtcag gtaaacggca
    11521 tgcaaaggat ccatagtgat ttcatccata aataagtgaa ctaactgcat catatttcta
    11581 ccaaaaataa tcgggtgcga gagagatcac aaagtgtctt atttccggtt actggcgttt
    11641 atgccctgac tgaactaatt attaatcaac ccaataatgt cggtgggtga tagtgtgata
    11701 acaactctgg agccgtaata tgaaaataat ctctaaaatg ttagtcggtg cgttagcgtt
    11761 tgccgttacc aatgtctatg ccgctgaatt gatgaccaaa gcggaatttg aaaaagttga
    11821 atcgcagtat gaaaaaatag gtgatatttc aaccagtaat gaaatgtcga ctgcagatgc
    11881 aaaagaagat ttaatcaaaa aagccgatga aaaaggggct gatgtgttgg tactgacctc
    11941 cggtcaaact gacaataaga tccacggcac ggcggatatt tataagaaga agtaattctg
    12001 aatcctatgt aaacatcccc gatgcgtaag tttatcggtg atcatctatt gaaatttatg
    12061 ccggataaag cgttcgcgct gcattcggca gttcagcttt tcagccgccg ccagaacgtc
    12121 gtccggctga tgcctaaata attcgccgct gctgttttat cgccattaaa tttctccagt
    12181 gcctgttgtg gtgtcagcaa gcgtggagcg ggaattttcg ccgactcgcg cgccagttcc
    12241 ggcagtagca gctgcaaaaa ctgcggcgtt aaatccggcg tcggttccac acttaaaaat
    12301 agcgccagtc gctccatcat attgcgcagt tcacgaatat tacctggcca gtcgtagtgc
    12361 accagcacgg tttcgcttgc ctgtaatccc tggcgtaatg cggcagaaaa tggggcggag
    12421 agcgccgcca gagacacttt caaaaagctt tccgccagcg gaagaatatc tgccacccgc
    12481 tcgcgcagtg gtggcaattg cagacgcaaa atactcagcc gataaaacag atcacggcga
    12541 aactgccctt gccgcatatc ttcttccaga ttgcagtgag tggcgctaat gacccgtaca
    12601 tctaccggaa caggctgatg cccgccgacg cgggtgacct ctttttcttc cagcacccgt
    12661 aacaggcgag tctgcaaagg tagcggcatt tcgccaatct catccagaaa cagcgtgcca
    12721 ccgtgggcaa tttcgaacag cccggcgcga cctccgcgtc gcgagccggt aaacgcccct
    12781 tcctcatagc caaacagctc cgcttccagc agcgattcgg caatcgcccc gcagttgacg
    12841 gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt ggcgggcaaa atattcccga
    12901 tgaattgcct gggccgccag ctctttgccc gtccccgttt ccccctcaat caacaccgcc
    12961 gcactggagc gggcatacag caaaatagtc tgccgcacct gctccatctg tggtgattga
    13021 ccgagcatat cgcccagcac gtaacgagta cgcagggcgt tgcgggtggc atcgtgagtg
    13081 ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc tgaacgcctg gcgcacggtg
    13141 gcggcggaat agataaaaat tccggtcatt ccggcttctt ctgccagatc ggtaatcagc
    13201 cccgcgccga ccaccgcttc ggtgccgtta gcttttagct cgttaatctg cccgcgtgcg
    13261 tcttcttcgg taatgtagct acgttgatcg aggcgcaaat taaaggtttt ttgaaacgcc
    13321 accagtgccg gaatagtttc ctgataagtg acaacgccga tagaagaggt gagttttccg
    13381 gcttttgcca gtgcctgtaa cacatcgtag ccgctcggtt taatcaaaat aactggcact
    13441 gacaggcggc ttttcaggta cgcgccgtta gagccagcgg cgatgatggc gtcacagcgt
    13501 tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt caaagccaag ctgaataggg
    13561 gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc gaaacagctc gaataagcgc
    13621 gttacagata ccgtccagat aaccggtttg tcgtcattca gccgtggtgg atgtgccata
    13681 gcgcaccgca aagttaagat accgaatatt gggtttagtc ttgtttcata attgttgcaa
    13741 tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac gcatatttgc ggattagttc
    13801 atgactttat ctctaacaaa ttgaaattaa agatttaatt ttattaaggc aattgtggca
    13861 caccccttgc tttgtcgtta tcaacgaaaa taacaagttg ataacaaagg atgggctatg
    13921 tctctacact ctccaggtaa agcgtttcgc gctgcactga ctaaagaaaa tccattgcag
    13981 attgttggca ccattaacgc caaccatgcg ctgttggcgc agcgtgccgg atatcaggca
    14041 atttatctct ccggcggtgg cgtggcggca gggtcgctgg ggctgcccga tctcggtatt
    14101 tctactcttg atgacgtgtt gacagatatt cgccgtatca ccgacgtttg ttcgctgccg
    14161 ctgctggtgg atgcggatat cggttttggt tcttctgctt ttaacgttgc gcgtacggtg
    14221 aaatcgatga ttaaagccgg tgcggcggga ttgcatattg aagatcaggt tggtgcaaaa
    14281 cgctgtggtc atcgcccgaa caaagcgatc gtctcgaaag aggagatggt ggatcggatc
    14341 cgcgcggcgg tggatgcgaa aaccgatcct gattttgtga tcatggcgcg caccgatgcg
    14401 ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc aggcctatgt tgaagcgggt
    14461 gccgagatgt tgttcccgga ggcgattacc gaactcgcca tgtaccgcca gtttgccgat
    14521 gcggtgcagg tgccaatcct cgccaacatt accgaatttg gcgccacgcc gctgtttacc
    14581 accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt acccgctttc agcgtttcgc
    14641 gccatgaacc gcgccgccga acatgtctac aacgtcctgc gccaggaagg cacgcagaaa
    14701 agcgtcatcg acaccatgca gacccgcaac gagctgtacg aaagcatcaa ctactaccac
    14761 tacgaagaga agctcgacga cctgtttgtc cgtagccagg cgaaataaaa aacgcccgtt
    14821 ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc gacttatcag gcctacgggg
    14881 tgaactgtag gtcggataag acgcatggcg tcgcatccga caacaatctc gaccctacaa
    14941 atgataacaa tgacgaggac aatatgagcg acacaacgat cctgcaaaac agtacccatg
    15001 tcattaaacc gaaaaaatct gtggcgcttt ctggcgttcc ggcgggcaat acggcgctct
    15061 gcaccgtggg taaaagtggc aatgacttgc attaccgtgg ctacgatatt cttgatctgg
    15121 cggaacattg cgaatttgaa gaagtggcgc atctgctgat ccacggcaaa ctgccgaccc
    15181 gtgacgaact cgccgcctac aaaacgaaac tgaaagccct gcgcggttta ccggctaacg
    15241 tgcgtaccgt gctggaagcc ttaccggcgg cgtcacaccc gatggatgtt atgcgcaccg
    15301 gcgtttccgc gctcggctgc acgctgccag aaaaagaggg gcataccgtc tctggcgcgc
    15361 gggatattgc cgacaaactg ctggcgtcac ttagctcgat tcttctctac tggtatcact
    15421 acagccacaa cggcgaacgc atccagccgg aaaccgatga cgactccatc ggtggtcact
    15481 tcctgcatct gctgcacggc gaaaagccgt cgcaaagctg ggaaaaggcg atgcatattt
    15541 cgctggtgct gtacgccgaa cacgagttta acgcctccac ctttaccagc cgggtgattg
    15601 cgggcactgg ctctgatatg tattccgcga ttattggcgc gattggtgca ctgcgcgggc
    15661 cgaagcacgg cggggcgaat gaagtgtcgc tggagatcca gcaacgctac gaaacgccgg
    15721 acgaagccga agccgatatc cgcaagcggg tggaaagcaa agaagtggtc attggttttg
    15781 gtcatccggt ttataccatc gccgacccgc gccaccaggt gattaaacgt gtggcgaagc
    15841 agctctcgca ggaaggcggc tcgctgaaga tgtacaacat cgccgatcgc ctggaaacgg
    15901 tgatgtggga gagcaaaaag atgttcccca atctcgactg gttctccgct gtttcctaca
    15961 acatgatggg cgttcccacc gagatgttca caccactgtt tgttatcgcc cgcgtcaccg
    16021 gctgggcggc gcacattatc gaacaacgtc aggacaacaa aattatccgt ccttccgcca
    16081 attatgttgg accggaagac cgccagtttg tcgcgctgga taagcgccag taaacctcta
    16141 cgaataacaa taaggaaacg tacccaatgt cagctcaaat caacaacatc cgcccggaat
    16201 ttgatcgtga aatcgttgat atcgtcgatt acgtcatgaa ctacgaaatc agctccaaag
    16261 tagcctacga caccgcacat tactgtctgc tcgacacgct cggctgcggt cttgaagctc
    16321 tcgaataccc agcctgtaaa aaactgctgg ggccaattgt ccccggcacc gtcgtaccta
    16381 acggcgtgcg cgtccccgga actcagttcc agctcgaccc cgtccaggcg gcatttaaca
    16441 tcggcgcgat gatccgctgg ctcgatttca acgatacctg gcttgcggca gagtggggcc
    16501 atccttccga caatctcggc ggcattctgg caacggcgga ctggctttca cgcaacgcgg
    16561 tcgccagcgg caaagcgccg ttgaccatga aacaggtgct gaccggaatg attaaagccc
    16621 atgaaattca gggctgcatc gcgctggaaa actcctttaa ccgcgtcggc ctcgaccacg
    16681 ttctgttagt gaaagtggct tccaccgccg tggtcgccga aatgcttggc ctgacccgcg
    16741 aggaaattct caacgctgtt tcgctggcgt gggtggacgg tcagtcgctg cgcacctatc
    16801 gccatgcgcc gaacaccggc acgcgtaaat cctgggcggc gggcgatgcc acttcccgcg
    16861 cggtacgtct ggcactgatg gcgaaaacgg gcgaaatggg ttacccgtca gccctgactg
    16921 cgcctgtgtg gggcttctac gacgtctcct ttaaaggtga atcgttccgc ttccagcgtc
    16981 cgtacggttc ttacgtgatg gaaaatgtgc tgttcaaaat ctccttcccg gcggagttcc
    17041 actcccagac ggcagttgaa gcagcgatga cgctctatga acagatgcag gcagcaggca
    17101 aaacggcggc agatatcgaa aaagtgtcca tccgcaccca cgaagcctgt attcgcatca
    17161 tcgacaaaaa ggggccgctc aataacccgg cagaccgcga ccactgcatt cagtacatgg
    17221 tggcgatccc actgctattc gggcgcttaa cggcggcaga ttacgaggac aacgttgcgc
    17281 aagataaacg cattgacgcc ctgcgcgaga agatcaattg ctttgaagat ccggtattta
    17341 ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa tgccattacc cttgagttca
    17401 ccgacggcac acgatttgaa gaagtggtgg tggagtaccc cattggtcat gctcgccgcc
    17461 gtcaggatgg tattccgaaa ctggtcgata aattcaaaat caatctcgcg cgccagttcc
    17521 cgactcgcca acagcagcgc attctggagg tttctctcga cagagctcgc ctggaacaga
    17581 tgccggtcaa tgagtatctc gacctgtacg tcatttaagt aaacggcggt aaggcgtaag
    17641 ttcaacagga gagcattatg tcttttagcg aattttatca gcgttcgatt aacgaagcgg
    17701 agcagttctg ggccgagcag gcccggcgta ttgactggca gacgcccttt acgcaaacgc
    17761 tcgatcacag caatccgccg tttgcccgtt ggttttgtga aggccgaacc aacttgtgcc
    17821 acaacgctat cgaccgctgg ctggagaaac aaccagaggc gctggcgctg attgccgtct
    17881 cttcggaaac agaagaagag cgcaccttta cctttcgcca gcttcatgac gaagtaaacg
    17941 cggtggcgtc aatgctgcgc tcgctgggcg tgcagcgtgg cgatcgggtg ctggtgtata
    18001 tgccgatgat tgccgaagcg catattactc tgctggcctg cgcgcgcatt ggcgctattc
    18061 actcggtggt gtttggggga tttgcctcgc acagcgtggc ggcgcgaatt gatgatgcta
    18121 aaccggtgct gattgtctcg gctgatgccg gagcgcgcgg tggcaaaatc attccctata
    18181 aaaaattgct cgacgatgcg ataagtcagg cgcagcacca gccacgccat gttttgctgg
    18241 tggatcgcgg gctggcgaaa atggcacgcg tcagcgggcg ggatgtcgat ttcgcgtcgt
    18301 tgcgccatca acacatcggc gcgcgggtac cggtggcgtg gctggaatcc aacgaaacct
    18361 cctgcattct ctacacctcc ggcacgaccg gcaaacctaa aggcgtgcag cgtgacgttg
    18421 gcggatatgc ggtggcgctg gcgacctcga tggacaccat ttttggcggc aaagcgggcg
    18481 gtgtgttctt ttgtgcttcg gatatcggct gggtggtggg gcattcgtat atcgtctacg
    18541 cgccgctgct ggcggggatg gcgactatcg tttacgaagg attaccgacc tggccggact
    18601 gcggcgtgtg gtggaaaatc gtcgagaaat atcaggttag ccggatgttc tcagcgccga
    18661 ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat tcgcaaacac gatctctcgt
    18721 cgctggaagt gctctatctg gctggagaac cgctggacga gccgaccgcc agttgggtga
    18781 gcaatacgct ggatgtgccg gtcatcgaca actactggca gaccgaatcc ggctggccga
    18841 ttatggcgat tgctcgcggt ctggacgaca ggccgacgcg tctgggaagc cccggcgtgc
    18901 cgatgtatgg ctataacgtg cagttgctta atgaagtcac cggcgaaccg tgtggcgtca
    18961 acgagaaagg gatgctggtg gtggaagggc cgctgccgcc ggggtgtatt cagaccatct
    19021 ggggcgacga cgaccgcttt gtgaagacgt actggtcgct gttttcccgc ccggtgtacg
    19081 ccacctttga ctggggcatc cgtgacgctg acggttatca ctttattctc gggcgcactg
    19141 acgatgtgat taacgttgcc gggcatcggc tggggacgcg cgagattgaa gagagtatct
    19201 ccagtcatcc gggcgttgcc gaagtggcgg tggttggggt gaaagatgcg ctgaaagggc
    19261 aggtggcggt ggcgtttgtc attccgaaag agagcgacag tctggaagat cgtgatgtgg
    19321 cgcattcgca agagaaggcg attatggcgc tggtggacag ccagattggc aactttggcc
    19381 gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac gcgatccgga aaaatgctgc
    19441 gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg ggatctgacg accattgatg
    19501 atcctgcgtc gttgaatcag atccgccagg cgatggaaga gtaaggtatt gtcggatgcg
    19561 tcgcgtggcg catccgacag ttgtgcgcag atgcctgatg cgacgc
//
LOCUS       DAZGXS010000085        19363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGXS010000085 DAZGXS010000000
VERSION     DAZGXS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(35..595)
                     /gene="pagP"
                     /locus_tag="ACHKG5_003151"
     CDS             complement(35..595)
                     /gene="pagP"
                     /locus_tag="ACHKG5_003151"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP0869202.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            1184..2569
                     /gene="dcuC"
                     /locus_tag="ACHKG5_003152"
     CDS             1184..2569
                     /gene="dcuC"
                     /locus_tag="ACHKG5_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0869203.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(2610..3290)
                     /gene="dpiA"
                     /locus_tag="ACHKG5_003153"
                     /gene_synonym="citB"
     CDS             complement(2610..3290)
                     /gene="dpiA"
                     /locus_tag="ACHKG5_003153"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0869204.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(3259..4917)
                     /gene="dpiB"
                     /locus_tag="ACHKG5_003154"
     CDS             complement(3259..4917)
                     /gene="dpiB"
                     /locus_tag="ACHKG5_003154"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0869205.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            5297..6355
                     /gene="citC"
                     /locus_tag="ACHKG5_003155"
     CDS             5297..6355
                     /gene="citC"
                     /locus_tag="ACHKG5_003155"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0869206.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            6370..6666
                     /gene="citD"
                     /locus_tag="ACHKG5_003156"
     CDS             6370..6666
                     /gene="citD"
                     /locus_tag="ACHKG5_003156"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0869207.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            6663..7571
                     /gene="citE"
                     /locus_tag="ACHKG5_003157"
     CDS             6663..7571
                     /gene="citE"
                     /locus_tag="ACHKG5_003157"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0869208.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            7582..9114
                     /gene="citF"
                     /locus_tag="ACHKG5_003158"
     CDS             7582..9114
                     /gene="citF"
                     /locus_tag="ACHKG5_003158"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0869209.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            9118..9669
                     /gene="citX"
                     /locus_tag="ACHKG5_003159"
     CDS             9118..9669
                     /gene="citX"
                     /locus_tag="ACHKG5_003159"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0869210.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            9644..10522
                     /gene="citG"
                     /locus_tag="ACHKG5_003160"
     CDS             9644..10522
                     /gene="citG"
                     /locus_tag="ACHKG5_003160"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP0869211.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            10573..12036
                     /gene="citT"
                     /locus_tag="ACHKG5_003161"
     CDS             10573..12036
                     /gene="citT"
                     /locus_tag="ACHKG5_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0869212.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            12150..12956
                     /gene="rna"
                     /locus_tag="ACHKG5_003162"
     CDS             12150..12956
                     /gene="rna"
                     /locus_tag="ACHKG5_003162"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0869213.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            13186..13596
                     /gene="rnk"
                     /locus_tag="ACHKG5_003163"
     CDS             13186..13596
                     /gene="rnk"
                     /locus_tag="ACHKG5_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0869214.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            13734..13823
                     /gene="yldA"
                     /locus_tag="ACHKG5_003164"
     CDS             13734..13823
                     /gene="yldA"
                     /locus_tag="ACHKG5_003164"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0869215.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(13827..15065)
                     /gene="ybdR"
                     /locus_tag="ACHKG5_003165"
     CDS             complement(13827..15065)
                     /gene="ybdR"
                     /locus_tag="ACHKG5_003165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP0869216.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQEGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            15286..15714
                     /gene="uspG"
                     /locus_tag="ACHKG5_003166"
     CDS             15286..15714
                     /gene="uspG"
                     /locus_tag="ACHKG5_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0869217.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(15835..17400)
                     /gene="ahpF"
                     /locus_tag="ACHKG5_003167"
     CDS             complement(15835..17400)
                     /gene="ahpF"
                     /locus_tag="ACHKG5_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0869218.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(17529..18092)
                     /gene="ahpC"
                     /locus_tag="ACHKG5_003168"
     CDS             complement(17529..18092)
                     /gene="ahpC"
                     /locus_tag="ACHKG5_003168"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0869219.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            18464..19210
                     /gene="dsbG"
                     /locus_tag="ACHKG5_003169"
     CDS             18464..19210
                     /gene="dsbG"
                     /locus_tag="ACHKG5_003169"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0869220.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         4710 a         5029 c         4692 g         4932 t
ORIGIN      
        1 tgattttaaa gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa
       61 agtacacatt gccattgttg taggtacccg gaatgtaggt catctgaaaa gtaactgggc
      121 cataacccac ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc
      181 gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc
      241 gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa
      301 atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac
      361 cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg
      421 catgccaggt gatggcagga atatataaat cataatgttc aggctgttgc caggtttgta
      481 caatattttc tctaaacgtt gtcatccact catctgcgtt agcaaaaact ttaccaacgc
      541 tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg
      601 accataaaac atttatcaaa aatctactac tagcatagca aagctacaat taacataacc
      661 ttaacagccc caacataaag aataatctga atagctgctc tatttatttc cgcttgttca
      721 agttaaatat ttccgcgcat cgatttaaaa tgacacatcg ttttctgcga tgggaatagc
      781 cataaaagaa aaagctagtt gtttctgaca acaaaatgca accaagaaaa aatgacttat
      841 tttttaataa gataccaaaa atcatcttaa cagggaatat attatatatc gtcaacccac
      901 gcccattgca gattattttt tgagcacaca accgcagcta acaccattgc aattaacaaa
      961 tttgcatcaa tcctccatca atttacacac attattatgt gataattgcc aaccgctaaa
     1021 tatgcgtttt gttatctacg tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc
     1081 ttgattttaa tcagcattca tcgccaattt attgggcata ttttttcctt aagctttagg
     1141 aattttttat tatttacttt ggggcctgga gacaggaaaa attatgctga cattcattga
     1201 actccttatt ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc
     1261 cgccactggt gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg
     1321 gcacaaagtg ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga
     1381 atacgttaaa atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct
     1441 gtgtggattt gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc
     1501 gtcaaaacca ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt
     1561 cgcctgtcta atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc
     1621 aaccctattt ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg
     1681 tgcctccccg gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca
     1741 agcttccgaa atgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc
     1801 tgcaattatc ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga
     1861 gcacatctct catgaaatgt tagatgtcag tgaaatcacc accactgccc ctgcgtttta
     1921 tgccattttg ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc
     1981 gcaattacac atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt
     2041 cctccgcagc tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat
     2101 ggcagatgcg tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg
     2161 gcttagcacc atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc
     2221 gagtatcatc ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg
     2281 ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc
     2341 cggcattaac ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggccgtac
     2401 cctttcgccc gtttctggcg tagtcgttgc ggttgccggg atggcgaaga tctcgccgtt
     2461 tgaagtcgta aaacgcacct cggtaccggt gcttgttggt ttggtgattg ttatcgttgc
     2521 tacagagctg atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc
     2581 ggaggcaagt ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgcggtctg
     2641 ccaactttgc cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga
     2701 taacgcctgg cagtggtgcg actgatggtc agtgcctgcg ccaccgtttc tgccgtatgt
     2761 tgcacaccag gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg
     2821 gtcggcagtt cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc
     2881 ttctggctgg cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc
     2941 gtttgcccca gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt
     3001 acggcttcag acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga
     3061 taatgcgcct gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta
     3121 tcgagcaaga ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccaggttt
     3181 cccgccagta atatctgact gaatccggga atgtgacgaa tatattcagc atgcatctct
     3241 gccagcggcg tttcgtcctc aacgatcaat agggttaatg gagctgtcat ttggtttcac
     3301 tttcggaata tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac
     3361 accaccgcag cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg
     3421 ctcgtcagca cgcgtactga ccccctgctc aaatattttg tctcgtagag actctggaac
     3481 gccgcagccc tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa
     3541 ttcaacgatc ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa
     3601 attgcccaca atggctgcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg
     3661 gctaccgggg acaatgatca tttttagccc cagttcccgg gcgcgctgca ctttaccaaa
     3721 aagcagcccc gccacctggc gatcggcaaa cgcctcgcgc aggctgtcaa taagctgttg
     3781 ctgggcctga gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa
     3841 cagaccattg agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc
     3901 aacgtattgt tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactgcg
     3961 aaagctgata atggccccca gcaaatcatc acctgaacga atagcttccc gattggcaat
     4021 aacgctcaga ccgttaaagt tagccaccac atcctgacgt ttttcatcaa tctgttcggt
     4081 aaagaaatcg gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg
     4141 agagctcagc cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg
     4201 atccaccgca atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac
     4261 gcgtgcgatt tgctttggct ccatgcccat catttgccga cggatatgcg ctgccaggaa
     4321 ccacgacagc aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa
     4381 aaactcagcc cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat
     4441 gacttttcca tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgaccc
     4501 tttcccggta atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat
     4561 aggataacca attttctccg gattaggatg gtaaaggcgg atcgagtgcc ggtccccaat
     4621 caccacataa tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt
     4681 gtagtcacgc gttttcacct cagagatgac actgtcattg gaggcaataa ttttcgcctg
     4741 attcattgcc atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata
     4801 ttgcgccagg gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag
     4861 cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt
     4921 tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat
     4981 acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa
     5041 acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta aataaataaa
     5101 aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc cttaggatgt
     5161 atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa
     5221 aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt
     5281 caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa
     5341 taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac
     5401 agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc
     5461 cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct
     5521 gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc atctgtttat
     5581 ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag
     5641 cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga
     5701 atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgcga tgaacgccaa
     5761 tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt gcgactggtt
     5821 gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggctggatct
     5881 ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat
     5941 catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg
     6001 ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac
     6061 tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat
     6121 gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg ttgaaattga
     6181 gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa
     6241 aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa
     6301 cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac
     6361 aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg
     6421 atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa tagcagcgtt
     6481 gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac
     6541 gtgcgcggcg tacagctgaa tgtcgatgat aaaggcgcac tggactgcat tttacgtgca
     6601 cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc
     6661 caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc atgttgtttg
     6721 tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct gatgccctga
     6781 tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc cgcatggttt
     6841 atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt gtaaacgcgc
     6901 tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg
     6961 tacgtctgcc gaaaaccgat accgctcagg atgttctgga tatcgaaaaa gagatcctgc
     7021 gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg gcgattgaat
     7081 ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta
     7141 tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa
     7201 ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg ggtattcagg
     7261 cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc
     7321 acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc
     7381 tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag
     7441 ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg
     7501 acggtccggt tatcgatcgt gcccgtctgg tgctctcccg tgcagaactt tccggcatcc
     7561 gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg
     7621 gtagcggcct ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcaccaaag
     7681 caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg
     7741 attcgtcgct ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc
     7801 ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg
     7861 atcctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca cattcgccag
     7921 ggcgtggtta cccgcattta tacctccggc ctgcgtggtc cactggcgga agagatctcc
     7981 cgtggtctgc tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt gcatctggta
     8041 cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg tgatgaattc
     8101 ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt
     8161 gatgccgaca acgcaaaaca ggttgtgatg cttaccgaag aactgctgcc ttatccgcat
     8221 aatccggcaa gcattgagca agatcaggtt gatttaatcg tcaaagttga tcgcgttggc
     8281 gatgctgcaa aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg cgaactgctt
     8341 attgcccgta gcgctgcgga tgtgattgtc aactcaggct acttcaaaga aggtttctcc
     8401 atgcaaaccg gcaccggcgg cgcatcgctg gcggtaaccc ttttcctgga agacaaaatg
     8461 cgtagccgcg atattcgcgc cgacttcgcc ctcggtggta ttaccgcgac gatggttgat
     8521 ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtac agagctttga cagccacgct
     8581 gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac
     8641 tggggttcga aaggtgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa
     8701 attgacaccc agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct
     8761 tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccgctg
     8821 gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac cccgggctcc
     8881 agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg tccggaactg
     8941 gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgcgaacgt
     9001 gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt
     9061 gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg
     9121 cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc
     9181 cgggatgaaa ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt tccactggtc
     9241 tcctttaccg tggttgcgcc tgggccgatt aaagacagtg aggtcacacg ccgaattttt
     9301 aatcatggcg tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag
     9361 caggctgcac tggtttccgc cagcgggccg gagggtatgt tgagcattgc cgccccggct
     9421 cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg
     9481 gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg
     9541 cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat
     9601 caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac
     9661 gttaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg
     9721 gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggtctcgtgg atcgcattaa
     9781 ctgcggtgcg cataaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca
     9841 gggctggcta ccccgtttca ttgaatttgg tgcctgtagt gcggaaatgg caccagaagc
     9901 ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac
     9961 cgcgggcgta aacacgcata agggcagcat tttttcttta gggctactgt gtgcggcaat
    10021 tggccgtttg cttcagctca accagttgct aacgccaaca accgtttgtt ctacggcggc
    10081 aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac aactgacggc
    10141 aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg
    10201 ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt
    10261 agatcctgaa ctggcattgc tcgatacctt gctcctactg atggcgatca acggcgatac
    10321 caacgttgct tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac
    10381 attattgcaa aaagggggca ttcgaacccc cgcagatctc gattatctcc ggcagttcga
    10441 cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac
    10501 ctggttttta gcacagattt aattatttaa gaacttgata aatttggaaa tattaatttt
    10561 cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg
    10621 gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca ggcatggcat
    10681 tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca
    10741 gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat
    10801 gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggcctg
    10861 gctggttttt ccagcactac ggtatggctg gtatttggcg catttatttt tgcattaggg
    10921 tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catgggcaaa
    10981 cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca
    11041 ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg
    11101 ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg
    11161 atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac
    11221 gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg
    11281 ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac
    11341 gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa
    11401 ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc
    11461 agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg
    11521 gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga aagacattac ccgctataac
    11581 agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa cggcctgact
    11641 cgttctggtt ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca
    11701 ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc
    11761 agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt
    11821 ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc
    11881 tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa
    11941 tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg
    12001 ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa
    12061 tcatcacgcc cgccaggtta gcccacctgg cgggcttttt tatgatttaa tagatagtat
    12121 tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc
    12181 tcgcggtttc tctgcttccc ttctcttctg ccaacgccgt agcgttgcag gcaaaacagt
    12241 atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc
    12301 aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca
    12361 aagctgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc
    12421 gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc
    12481 taccagaagc gcgcgccagc cggatgtgtt catcgccgga aaccggatta tcactggaaa
    12541 cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct
    12601 acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga
    12661 tggtacgcct gaatcaagag ataaaagaga gcgaagccgg aaaattcctt gcggataatt
    12721 acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag
    12781 agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc
    12841 aggtctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac
    12901 aaccacaccc aggtaactgt ggcaaaacct ttgtgattga taaagcgggt tattaatcct
    12961 ctgatttaca aaacacgcta attcgtcttt cataaaccat gacgattaag aattatacat
    13021 aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac
    13081 agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg
    13141 tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact
    13201 atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat
    13261 gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg
    13321 ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt
    13381 agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat
    13441 actcagcttt ccgttatggc tccggtaggt gccgcactgc tgggactgcg cgttggcgat
    13501 tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa
    13561 taccagccag aagctgctgg cgactacctg ctttaatccc atctgtacaa gcgataactc
    13621 gctttgccca gaggatgcac tcgcatcctc tttatgcaaa atcagcaatt tttcctggtt
    13681 ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag
    13741 aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc
    13801 tttacctcga aaaccactcc tgacgctcat attgttcccc ccggcatcgc attcaccaga
    13861 cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag aatcacctta
    13921 cggcactcct cttcacgctt ttcaaagatc tcatatcccc gggcggcctc ttcaaacggc
    13981 atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat
    14041 tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag
    14101 gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg
    14161 ccgccacgcc tgaccgccgc aatacactga cgcaacgctt taccgctgct gccctccagt
    14221 ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct
    14281 attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg
    14341 ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa acgcaagcgg
    14401 tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt
    14461 gtcaacaatc ccacaggacc ggcaccatag acagcaacgc ttgaaccttc ttggatctgc
    14521 gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca
    14581 tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac acggacatac
    14641 tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa tgccgcggga
    14701 gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg
    14761 gcatattgct gcagtcgaca gaaaaaacag tcgccacaag caatgacgaa cggaattacc
    14821 acccggtcgc ctttttgcaa attttttaca tcctttccgg tttcaactac ttcccccata
    14881 aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg ataaagatgg
    14941 aggtcagagc cacagatcgc cgttgccgtt atacgcagaa taatatcatc tgcctgttca
    15001 atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct
    15061 ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag
    15121 cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt gagaatatat
    15181 tcatcacaga aacagttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat
    15241 aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc
    15301 attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa
    15361 ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc
    15421 ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa
    15481 gcagaagaac gtttgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa
    15541 caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttagcaga agaactgggg
    15601 gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct gttaggttct
    15661 aacgcctcga gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg ctaacagata
    15721 aaagtattta tgccgccctg cctggaacat cccggcaggg ctttttaatg ccataaaaaa
    15781 gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc
    15841 agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt
    15901 ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga acacgccttt
    15961 cacgttggtt tcgcatttcg catcaatgat aatctcgccc atgcggttac gttcgactgc
    16021 gccttcgagc cagttggtgt tcggcagcag accaatctgc acgaaaatac cggccagttc
    16081 gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc
    16141 gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg
    16201 cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac
    16261 gtgctcaacg atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat
    16321 aaccgctacg cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc
    16381 tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc
    16441 cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc
    16501 accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc
    16561 gtattcatca acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac
    16621 agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat
    16681 cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc
    16741 ggaaccgacg attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt
    16801 ttccgcgccc gtatcaattt tggcaacgat ttcagtcaaa gtcatgcggc cctgaccaaa
    16861 ctctttcccg ttcacgaaca ctgccggaac gcccatcacg ttacgatcag tgatctcgtt
    16921 ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata cgctcatcag
    16981 gttcagcgcc tgtaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa
    17041 ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg
    17101 atgaccaccg gtccacagta acgccagtac cagcgaggtg aactcgtggc ccagcgggga
    17161 gcctgcaaaa cgtggcccct ggttagaacc tgggttggtg atcaggaaag acggcttacg
    17221 caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc
    17281 caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac
    17341 aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat
    17401 gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca
    17461 gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgatta attacgcgat
    17521 tgcggaactt agattttacc aaccaggtcc agagacggag ccagagttgc ttcaccttct
    17581 ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc tgctttgatt
    17641 ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc gattgcctgg
    17701 atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc atcttcacgc
    17761 atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt
    17821 ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt atcggtagat
    17881 actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc aacgtcaccc
    17941 agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac gctccagcgg
    18001 ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc ctggttttta
    18061 aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt ttcgatgaga
    18121 tgtaaggtaa ccaattttta ccgattcggc taatgcgttt ccattatcaa atcgataagt
    18181 tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc ggcgataaaa
    18241 agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag
    18301 cgccagtctg caggctgcgt tgttgctctt acaaatgcaa caacgacatg gattacaaca
    18361 ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacaa tgataggtta
    18421 tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa aaaagatact
    18481 tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgctc cagtaaaagc
    18541 gattgaaaaa cagggcatta caatcatcaa aacgttcgat gcccccggag gaatgaaagg
    18601 ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag atggtaagca
    18661 cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca cacttatcga
    18721 aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac aatcccactg
    18781 gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg atccgttctg
    18841 cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg gcaaagtaca
    18901 gttgagaaca ttgttggttg gggttatcaa accagaaagt ccggcgacag ccgcggcaat
    18961 tcttgcctcc aaagatcccg caaaaacctg gcaagaatat gaagcctctg gtggcaagct
    19021 taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa gcgacaacga
    19081 gaaactcatg gacgatctgg gggcaaatgt cactccggct atctattaca tgagtaagga
    19141 aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata tcattatggg
    19201 aaataaataa aaggtaattc atggatggtt gaactatatc cctgcttttc aaccatccta
    19261 aaaaagcatg aattgctcgc tctgccctaa gtggctgtgc tattttagaa aaacttaatc
    19321 ctgagttaag caaaaaaaac ataatcaata aaaatatata cgc
//
LOCUS       DAZGXS010000086        19244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGXS010000086 DAZGXS010000000
VERSION     DAZGXS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            12..530
                     /gene="yfgH"
                     /locus_tag="ACHKG5_003170"
     CDS             12..530
                     /gene="yfgH"
                     /locus_tag="ACHKG5_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0869221.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /gene="yfgI"
                     /locus_tag="ACHKG5_003171"
     CDS             546..1085
                     /gene="yfgI"
                     /locus_tag="ACHKG5_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0869222.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="ACHKG5_003172"
     CDS             complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="ACHKG5_003172"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0869223.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="ACHKG5_003173"
     CDS             complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="ACHKG5_003173"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0869224.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4452..5822
                     /gene="xseA"
                     /locus_tag="ACHKG5_003174"
     CDS             4452..5822
                     /gene="xseA"
                     /locus_tag="ACHKG5_003174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0869225.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5819..6034)
                     /gene="yfgJ"
                     /locus_tag="ACHKG5_003175"
     CDS             complement(5819..6034)
                     /gene="yfgJ"
                     /locus_tag="ACHKG5_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0869226.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6103..7575)
                     /gene="der"
                     /locus_tag="ACHKG5_003176"
     CDS             complement(6103..7575)
                     /gene="der"
                     /locus_tag="ACHKG5_003176"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0869227.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="ACHKG5_003177"
     CDS             complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="ACHKG5_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0869228.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="ACHKG5_003178"
     CDS             complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="ACHKG5_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0869229.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="ACHKG5_003179"
     CDS             complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="ACHKG5_003179"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0869230.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="ACHKG5_003180"
                     /gene_synonym="gcpE"
     CDS             complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="ACHKG5_003180"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0869231.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="ACHKG5_003181"
     CDS             complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="ACHKG5_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0869232.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13348..14502)
                     /gene="rlmN"
                     /locus_tag="ACHKG5_003182"
     CDS             complement(13348..14502)
                     /gene="rlmN"
                     /locus_tag="ACHKG5_003182"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0869233.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="ACHKG5_003183"
     CDS             complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="ACHKG5_003183"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0869234.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15224..16078)
                     /locus_tag="ACHKG5_003184"
     CDS             complement(15224..16078)
                     /locus_tag="ACHKG5_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0869235.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16078..16899)
                     /locus_tag="ACHKG5_003185"
     CDS             complement(16078..16899)
                     /locus_tag="ACHKG5_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFP0869236.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16892..17521)
                     /locus_tag="ACHKG5_003186"
     CDS             complement(16892..17521)
                     /locus_tag="ACHKG5_003186"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFP0869237.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17518..>19244)
                     /locus_tag="ACHKG5_003187"
     CDS             complement(17518..>19244)
                     /locus_tag="ACHKG5_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFP0869238.1"
                     /translation="TLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRY
                     SDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVP
                     ANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRH
                     ICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNW
                     MQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIE
                     FIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDY
                     DWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKS
                     APHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLT
                     AKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDR
                     GVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQT
                     MLVEVEKA"
BASE COUNT         4754 a         5231 c         4687 g         4572 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat tccggaacga
      301 cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacgt ataaggaagg caccaaagtg tatacctcta
      421 ctcaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacgacgt
      481 ataacgaaac gcgtattcag ccaaatacca aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggctgccg ctaacaaaag agcggcaacg gcagcggcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcaa
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggagctg aagcacaaag
      961 ccgcgcaaac tgatgtcgtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa taaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaagcctat aattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca atggtagctg
     1201 gtggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc
     1261 ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt
     1321 cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta
     1381 cgccaacgga acgtactggc aggaagacgg tgaacgcctg gctgactttg tcgtacaggt
     1441 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact
     1501 ctttcttcac ttcacccaga acacgaacgc caaggcctgg tcccgggaac gggtgacggt
     1561 acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
     1621 gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt
     1681 ggtgagattt gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg ataacgtcag
     1741 ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
     1801 atacttcaac gaaaacgcga ccgatgattt tacgttttgc ttccggatcg ttttcgccag
     1861 ccagcgctga caggaagcga tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat
     1921 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
     1981 atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat
     2041 ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag
     2101 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
     2161 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
     2221 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
     2281 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
     2341 catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc
     2401 tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt
     2461 gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc
     2521 ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc
     2581 cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc
     2641 atgcccacag ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt
     2701 actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
     2761 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
     2821 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
     2881 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
     2941 gatagtacca cagccggtca aacccataca ggagcgcagg ccgcccatct gctggtgaat
     3001 gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt
     3061 gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag
     3121 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
     3181 agattcttca gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagcggcgat
     3241 agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt
     3301 gccttccagg gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt
     3361 aacgatacga gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc
     3421 agcttctgcc agagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
     3481 atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg
     3541 ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc
     3601 gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca
     3661 ggcgttcggt ttacgttccg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg
     3721 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
     3781 cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac
     3841 gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatccccac
     3901 cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc
     3961 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga
     4021 ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
     4081 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
     4141 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
     4201 gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa
     4261 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
     4321 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
     4381 gcttgctatc cccgaagggc gggttactat cgactgaata acctgctgat ttagaatttg
     4441 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc
     4501 aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct
     4561 ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc
     4621 aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgc ccacagcatg
     4681 ggcaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga
     4741 taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc
     4801 tcaaagcgaa gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct
     4861 cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt
     4921 tgcatgtgtt aaaacgtcgc gatccttctc tgccggtgat catctacccc accgccgttc
     4981 agggcgatga cgcgccgggg caaattgttc gcgccattga actggcgaat cagtgcaatg
     5041 agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta
     5101 acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg
     5161 ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgca ccaacaccgt
     5221 cggctgccgc cgaagtggtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga
     5281 cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgcaca cgtcgcttta
     5341 cgcaaattca tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa
     5401 ccatgcttga acgcctgcaa aaacggatga gctttgcgct ggaaaatcaa cttaagcgtg
     5461 ccgggcaaca gcagcagcga ttaacacagc ggctgaatca gcaaaatcca cagccgaaga
     5521 ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg
     5581 cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa
     5641 gcccactgtc aacgctggcg cgtggataca gcgttactac tgctactgac ggcaaggtac
     5701 tgaaaaaagt gaagcaggtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct
     5761 gggtagaaag tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaagtgcatt
     5821 aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt
     5881 aatctaccgc accgcaggct tttaacacct gcaacggttg atggcagtcc gggcaaagag
     5941 ctttcatttc aataactttc ccgcaggatg ggcaatacgc atggccgtta tcctgatcaa
     6001 gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta gccacccctc
     6061 tacattcata gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat
     6121 gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt
     6181 cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag
     6241 cgatttgcgg aagtagttca tcaagtaacg cttgtaggaa tcaggcaggt ctttcacctg
     6301 attaccgtga atcaccacaa tcggcggatt ataaccaccg gcgtgtgcat atttcagctt
     6361 cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg
     6421 cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc
     6481 aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc
     6541 gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt cctgactcag
     6601 gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag
     6661 cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt
     6721 ggcgtcttca atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat
     6781 tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc
     6841 cattggaatg tagatactgt cacgcgtcgt accaggcatg tcataaacaa caacgcgctc
     6901 ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc
     6961 cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc
     7021 ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag
     7081 atcttccatc cacggcagta gcacatgctc cagcagactt aatacgccac gaccgtgaga
     7141 cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc
     7201 gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag
     7261 atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag
     7321 tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc
     7381 gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc
     7441 cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg
     7501 ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc
     7561 gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata
     7621 ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa
     7681 cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag
     7741 tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc
     7801 aacgaaacga ccatcttcga cgttaatcca gtgcaaataa ccttcactgt caccgaccac
     7861 caggttgcca ttatacagca ccggagaagt cagcaggcga tgcagcagat cgctttgtgt
     7921 ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag
     7981 atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat
     8041 aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac
     8101 aacgccgtta acaacggcgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga
     8161 accggtcgct tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc
     8221 gctgacgcga ccattatcgc cccccacgac ggccgcacca aaagccgttg ccggcgcaga
     8281 ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc
     8341 agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct
     8401 gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc
     8461 gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gtccaccaga
     8521 cacggtcaca ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc
     8581 caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc
     8641 gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt
     8701 gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac
     8761 ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct
     8821 taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct
     8881 ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat
     8941 cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca
     9001 atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa
     9061 gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat
     9121 ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg
     9181 cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg
     9241 caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt
     9301 tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat
     9361 caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc
     9421 caacagccag tgctttgcca ttttcagcaa aaaagcgttt aatcgcttct acctggtcgt
     9481 tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa
     9541 atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac
     9601 tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca
     9661 tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt
     9721 cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc
     9781 agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac
     9841 ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc
     9901 acggccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg
     9961 ggagccgaga ctgttagtca cccactcgaa aacggtacgg ttgtagtaat ccagaccacg
    10021 caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc
    10081 ggcaaaatgc tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag
    10141 aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg
    10201 gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc
    10261 gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc
    10321 ggaaataccc agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc
    10381 cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt
    10441 ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta
    10501 cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt
    10561 caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac
    10621 ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact
    10681 gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt
    10741 ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat
    10801 tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagccatgc
    10861 gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc
    10921 tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcctcc
    10981 agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagaga
    11041 ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acccgggcca
    11101 ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc
    11161 tccagcgcgt taactgtacc gataacatca aattcctgac gcgaacaggt cggacaggcg
    11221 atgaagttga tacctcgcga acggatacga agcgatttca aaatatcgaa accgactttg
    11281 atctcttcga ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac
    11341 agcagcagac ctaaaccaat ggcggatttt actgccccgc tgcgcgcgcc acccgcttcg
    11401 gtgatcccca gatgcagcgg ctgatcgatt tgttttgcca gcaaacgata agactcaaca
    11461 gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg
    11521 agatgatcaa catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac
    11581 ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt
    11641 ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg
    11701 ttaatacgca gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag
    11761 tggatgtcag ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc
    11821 gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg
    11881 atttgattga ccgttgcttc gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg
    11941 atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt
    12001 tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg
    12061 gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca
    12121 gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt
    12181 tgtacggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca
    12241 attttttacc ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca
    12301 gcgcattcgg atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag
    12361 caccatctgg cgttgttgct gccgttggtg ccggggtcgc ggcggtatca acatttgcct
    12421 gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacga
    12481 caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag
    12541 ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc
    12601 tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt
    12661 cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg
    12721 tcatcagcca gccgtcgcgt tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg
    12781 caacttttgc agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg
    12841 gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat
    12901 cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct
    12961 cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg
    13021 tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt
    13081 aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta
    13141 agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca
    13201 ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg
    13261 ttaatattga ccagacaatt gcaacttaat acttcattca taaaacatta cgccacggta
    13321 cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg
    13381 catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca
    13441 ggcagcatcg atatcatcac cacgcgtttt acgcacaata gtggtgaaac cgtagctcat
    13501 cagcactttt gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcagccgg
    13561 gaacgggttc cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag
    13621 ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac
    13681 tcggccctga ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc
    13741 gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg
    13801 cagggaaatt gccagcgcaa catcgatcat atcgcccagt ttatccagcg ctggaactac
    13861 accggaagtg gaaagcgtca cgcgacgttt agacagacca aaaccgaaat catcaagcat
    13921 gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat
    13981 cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc
    14041 acgccacacc tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc
    14101 ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtagc
    14161 gcggtcgtct tccgggatgt acaccgtttc gacacgctgg tcacccacgg caatcgccca
    14221 tttaatggtg ccgtcagatg aacgctgttc ttcaaccact tccggtgcgc ggatctccgc
    14281 cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaaattgtc
    14341 gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa
    14401 atctttaaaa aactcccgca tctgctgacg attgagatcc agcaggttga tttttccatc
    14461 tttggtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt
    14521 tattacacgt tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc
    14581 gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat
    14641 ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt
    14701 tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca
    14761 gcgtaatcag cgcgcagagt accggccagt gcgtttgccg gattggtcgc gcccagcaga
    14821 tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag
    14881 gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca
    14941 cgtgcctgtt caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct
    15001 tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgataatg
    15061 gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc
    15121 agcgtacgag atggcgcgga ttataatgag caacagagcc gttgactatt gatgaaggta
    15181 acattttttt aaaataaaat gagttttagc aacaactcga cttttatggt gttctcatcc
    15241 cataacgatg acgttggcaa taaagacaat tttgcgcatt ttgctgaaaa gtaagaaagg
    15301 atttttgaca ggttttacag actttgcgat tagcatgaag attcacaatt tttgccggta
    15361 gaatcgtcaa ttcaacggtc attgcctgat gaaaacaaac agcaatacaa ttttggcatc
    15421 cgttacacaa ggcatcattt atttgtaaat ggccttcgtt aagcgaaaag acctgttgct
    15481 cgcaggcccg ccagcatgca ccgcaaagct gacacttttg aggatccatc atcgggcgag
    15541 catcactaat ctggggccat aaataatgta accgacgtaa tcctggttgt atttgtgtca
    15601 tctgaacatc cgcacgctgc acatggaaca aacgacgccg ggaaacattc acctcactgg
    15661 tatgtggaga taccacagac cagccttcct cgccatattg ccgtaagcat tcgtttaaca
    15721 gcaataatgc gcgctcccat gccttcgcct gactcagatc aatcgcgatc gctgtgatcc
    15781 cctgctcttt atgccatagc aaaagttctt ccagcgtcgg cgcaatagtg gtgaaaggtg
    15841 cgaccagggt gtgattgcaa aaataacgta aaggggaatt aacgccacga atagcctctt
    15901 gcggacaaat aaactggcaa accgcacaat caatacattg ttccgcgtcg accgctatgc
    15961 cctcttctgt cagcgttagc gcctgtgccg ggcaagcctc gacacaggca tgacatgttg
    16021 caaaacgaaa gcgttggcgc gcgcattccg cgccaatcgt tatgttatga cttaacatta
    16081 gccgatacta aaaaagacaa aacgcagcat aacttcactc cccactagca ttacgctacc
    16141 gaataacgcc gccgggatat tctttttaca accaccgaca gcaaagaaca tcatcccaat
    16201 gacggaaatt ccccagctta gtgtcagtat gggttttaac tgtcccagca tcgctaatgg
    16261 cgcatgtggc aatgtggcta tctgcatcgc atttgccgtt aaatccccta tccacaacgg
    16321 ctgaaccaac aaacgcagca gaaccaggag agcgatcacc aacacgcatc cattacgcag
    16381 ggcatcaatc tgcggcaaaa ttccgctaat acgaaacact gctaccagcg ctgaaccgag
    16441 aattcccact gtacctaaga acataatcag cgtgttgtaa tgctgccagg tgataatcga
    16501 cgtatggatg taaacttgcg ccatgcagaa aacatcaaca atcccgacca ctgccgctag
    16561 cataagcagg agttgccagc cgcctttgcg taacatgacc agtaatgtgg ataaacccaa
    16621 tagcgccaga tacagcgcac caaagataat ttcccgactc agccatgaac tggaaaagtg
    16681 gctcagcgca tgcatcgcat taagtggata acccaggtgt aaggtagaaa tcagtaaccc
    16741 tacggcacct gccaaaaaag ccaacagtaa tggcggccgc ataaccgggc gggcgctgga
    16801 ggtgattaaa atatgacgat gaagaaacgc cagccacaac gtcataccga ccgatgcctg
    16861 taacaacagc gtaaaagcca ttaacggtaa ttcatgcatg atgtgacgcc tccttttccg
    16921 cgccctgatg cggtttaatc accagatttg gctgcgtaat cgacgaatca ggtaaacctt
    16981 tcacatcact gacatcgcca tacttttcac gcaactcatc aatagggccg aacttaatcg
    17041 cccccaacgg gcacgttgcc acgcaaatgg gttgttctcc tttagcttgc agatcaatac
    17101 agaaatcgca tttcgacatc tgcccggtct gctcattcat ttgtggtgcg ccgtaaggac
    17161 aggaccaggc gcagtaaccg caaccgacgc acttatcagt attcacgcgc acaataccgt
    17221 ctccgggacg cttatgcatc gccatcgtcg ggcaattttt ggtacagatc ggatccgcgc
    17281 aatggttaca ggaaacagaa agggtataag caaagacatt atgactcaca ccgccctctc
    17341 cagtggcgat gaagccacct ccgttcactt catacacgcg gcgaaaacgt cgcccaacct
    17401 caaggttgtt tttgtcttta caggccacct gacacgcttt acagcccgaa caacgcgaag
    17461 agtcaataaa gaaccctaat tgtttgtcgc tgacagatgc aaattcttta aattggctca
    17521 tgctttctca acctccacca gcattgtttg atgcgaattc cctttcgcca gcgccgtaat
    17581 gcgggtcgaa ctcagtacat tggcacagcc tccgcgatcg ataccttgtg catccggttg
    17641 ccaccatgcc cccgcctgca tagcaactac gccagggatg atacgggggg ttatttccac
    17701 cgggataatg ctcacaccac gatcgttata aatttttacg ctatctcctt cgctaatccc
    17761 gcgctgtttt gcatcttgcg gattcagcca gagtttttgc gtctgaactt cttgcagcca
    17821 cgggttagca tactgggtgg aattagcacg gtttttccct ttccaggtga tgagctgcaa
    17881 cgggtatttc gccgtcagtt tatcttctgg cccttcaaag gcagggacat agtgcgaaag
    17941 cgcgggaatt tctggatctt gcatatcaaa aagacgctta gagaagatct caattttgcc
    18001 ggaaggcgtc gggaaaggat tattctgcgg atcgcgaata tttgcttcga aagcgatgtg
    18061 cggcgcgctt ttgaaaagat gtcgacgtgt tttttgcagg gttgcgaagt ccggcaaatt
    18121 ttcatccggc atcgccagac gagtttgctc ccagatatgc tcgatccatt gcttctcatc
    18181 acgtccttga ctgaactcgg cttcgacacc cagttttttc gccacgtcac gcaaccagtc
    18241 ataatcggtg cgacgttcaa aatccggctc aatcagtttt tctgacagaa taagatagct
    18301 cgcagttccc catgtttcgc cgatattcca gcgttccata aagctggttt ccggcagcaa
    18361 cacatcggca tatttcgcgc tcggagtcat gaacagatcg ctgagtacga tgaattctat
    18421 tttagattcg tcttcaagga gcttcgcggc ctggtggacg tccgggttct ggttcgccag
    18481 ataattacct gccagtgaga acagcaggcg gatattactg tcgagtttat cgacaccttt
    18541 aagcccatct tgcggcgtaa ctttagaagc atcatccgcc gcctgcatcc agttcataat
    18601 cgagattttc gcttgtaccg gattttctgg catatctggt ccaacgcaga atttacgatt
    18661 agaactaccg ccgtaacctg ccgcccagcc acctttgatg ccgacgttac cggtaatcga
    18721 ggccagtaaa gttgaacctc gggcggtgcg ttcgccgcaa atatgtcgct gtggccccca
    18781 tccctgaatc aacgctgcgg gttttgtagt ggcgtaatcg cgtgccagtt gacgaatgga
    18841 ttgtgctgga acgtgggtaa ttttttctgc ccattccggc gttttgtgta tcccatcttt
    18901 tgcgccaaaa aggtaggcaa ccaacgattc gttggctggt acgccttccg gcatggagtt
    18961 ttcatcaaaa ccgagagtat atgtgtcgat aaatgtctta tcgtggagat tctcgctgat
    19021 aatgacgtac atcatcgcgt ccatcaacgc attgtccgtg gtcggcaaca gcgggatcca
    19081 ctgatcggca agtgaagata cggtgtcgga atagcgcgga tccaccacga taaaacgtgt
    19141 accgttttgc ttcatttttt gaaaatagta attggtgtga ccgaatatgg tttccgttgg
    19201 gttatgcccc cagagaatca ccagtttggt atccagaaga gtat
//
LOCUS       DAZGXS010000087        19214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGXS010000087 DAZGXS010000000
VERSION     DAZGXS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(300..1949)
                     /gene="pgi"
                     /locus_tag="ACHKG5_003188"
     CDS             complement(300..1949)
                     /gene="pgi"
                     /locus_tag="ACHKG5_003188"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0869239.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2186..2382
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2474..3823
                     /gene="lysC"
                     /locus_tag="ACHKG5_003189"
     CDS             2474..3823
                     /gene="lysC"
                     /locus_tag="ACHKG5_003189"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0869240.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4076..4348
                     /locus_tag="ACHKG5_003190"
     CDS             4076..4348
                     /locus_tag="ACHKG5_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0869241.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4349..5221)
                     /gene="rluF"
                     /locus_tag="ACHKG5_003191"
     CDS             complement(4349..5221)
                     /gene="rluF"
                     /locus_tag="ACHKG5_003191"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0869242.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5569..6516
                     /locus_tag="ACHKG5_003192"
     CDS             5569..6516
                     /locus_tag="ACHKG5_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP0869243.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6586..7392
                     /locus_tag="ACHKG5_003193"
     CDS             6586..7392
                     /locus_tag="ACHKG5_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0869244.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRTDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7402..7809
                     /locus_tag="ACHKG5_003194"
     CDS             7402..7809
                     /locus_tag="ACHKG5_003194"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP0869245.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7809..8303
                     /locus_tag="ACHKG5_003195"
     CDS             7809..8303
                     /locus_tag="ACHKG5_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP0869246.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8369..9166
                     /locus_tag="ACHKG5_003196"
     CDS             8369..9166
                     /locus_tag="ACHKG5_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP0869247.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9177..10001
                     /locus_tag="ACHKG5_003197"
     CDS             9177..10001
                     /locus_tag="ACHKG5_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP0869248.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10053..11282
                     /gene="sorE"
                     /locus_tag="ACHKG5_003198"
     CDS             10053..11282
                     /gene="sorE"
                     /locus_tag="ACHKG5_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP0869249.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11665..12354
                     /gene="pepE"
                     /locus_tag="ACHKG5_003199"
     CDS             11665..12354
                     /gene="pepE"
                     /locus_tag="ACHKG5_003199"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0869250.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12445..14076)
                     /locus_tag="ACHKG5_003200"
     CDS             complement(12445..14076)
                     /locus_tag="ACHKG5_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0869251.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14296..17979)
                     /gene="metH"
                     /locus_tag="ACHKG5_003201"
     CDS             complement(14296..17979)
                     /gene="metH"
                     /locus_tag="ACHKG5_003201"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0869252.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18179..19003
                     /gene="iclR"
                     /locus_tag="ACHKG5_003202"
     CDS             18179..19003
                     /gene="iclR"
                     /locus_tag="ACHKG5_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0869253.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4538 a         4999 c         5040 g         4637 t
ORIGIN      
        1 gtattttccg aaatcaatca cgattaaaaa tctattttag gattaacctc acggtatgat
       61 ttccgttaaa tcacagacaa ggggatttat ctgataaaac aatgccgact tatagtcggc
      121 attgttttta aggttattta attaatcgtg ctatgcatta attgccgaat acgatgtaca
      181 tatcggcatc gacctgtagg cctgataaga cgcgtaaagc gtcgcatcag gcaccagttg
      241 ccggatgcgg cgtaaacgcc ttatccggcc tacatagcgg catcaggcat gaacgatgat
      301 taaccgcgcc acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg
      361 atttctttat catctttcag ctctggcaga atacggttcg ccagctgttt acccagttcc
      421 acgccccact ggtcgaaggt gaagatgttc aggatcacgc cctgagtaaa gattttgtgc
      481 tcatacagcg caatcaacgc acccaggctg aacggagtga tttcgcgcaa caggatagag
      541 ttggtcgggc ggttaccttc gaacactttg aacggcacca cgtagtcaag cgttgccgga
      601 tctttaccct gatcgcgata ttcctgctca accacttcgc gggatttacc aaacgccagc
      661 gcttcggtct gggcgaagaa gttagacagc agtttctggt ggtgatcaga gagcgggtta
      721 tgggtgatag ccggagcgat gaaatcgcac ggtaccattt tggttccctg gtggatcagc
      781 tggtagaacg cgtgctgacc gttagtgcct ggttcacccc agataatcgg gccagtctgg
      841 taatccacaa cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc
      901 tggaagtacg ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc
      961 gcaccaaaga aattgttgta ccagatgcca atcagcgcca acagtacagg caggtttttc
     1021 tcggcaggcg tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg
     1081 aagttatcaa agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa
     1141 cgaccgccga cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca
     1201 acggctttgg cattggttga gagcgccgca aagtgtttcg caacgtgttt ttcatcacct
     1261 gccgctttca ggaaccagtc acgcgcgcta tgggcgttgg tcatggtttc ctgagtggtg
     1321 aaggttttag atgctaccag gaacagcgtg gtttccgggt ttactttttt cagcacttca
     1381 gcgatgtgag tcccatcgac gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga
     1441 cgcagtgctt cggtcaccat gtacgggccg aggtcagaac cgccgatccc gatgttcact
     1501 acgtcagtga ttgctttgcc ggtataaccc ttccactcac cggagataat cgcttccgag
     1561 aaggttttca tcttctccag caccgcgttg acttctggca taacatcttt gccatcaacc
     1621 agaatcgggg tattgctacg attacgcagc gctacgtgca gcacggcgcg gttttcagtg
     1681 cggttgatct tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc
     1741 gccagatctt gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga gtaatccacc
     1801 agcatctgat cgtcgaaggt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa
     1861 agatcggcga tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca
     1921 gcggtctgcg ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg
     1981 actttggaag attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt
     2041 aatgttgagt gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca
     2101 ggagtggatg aaaaatccgc atgaccccgt cgttgacaac cgccccgctc accctttatt
     2161 tataaatgta ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg
     2221 ttggaggagc cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg
     2281 ctggccacgt tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt
     2341 cgcagtcggg cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc
     2401 gctctgcgcc cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg
     2461 acacgaggta gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga
     2521 ttttgacgcc atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt
     2581 tgtcctctcg gcttctgctg gtatcactaa tctgctggtc gctttagcgg aaggactgga
     2641 acctggcgag cgattcgaaa aactcgacgc tattcgcaac atccagtttg ccattctgga
     2701 acgtctgcgt tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac
     2761 tgttctggca gaagcggcgg cgctggcaac gtctccggcg ctgacagatg aactggtcag
     2821 ccacggcgag ctgatgtcga ccctgctgtt tgtcgagatc ctgcgcgaac gcgatgttca
     2881 ggcacagtgg tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga
     2941 gccagatgta gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gtctcaatga
     3001 aggcttagtg atcacccagg gatttatcgg tagcgaaaat aaaggtcgta caacgacgct
     3061 tggccgtgga ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg
     3121 tgttgatatc tggaccgacg tcccgggcat ctacaccacc gatccacgcg tagtttccgc
     3181 agcaaaacgc attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc
     3241 aaaagtactg catccggcaa cgttgctacc cgcagtacgc agcgatatcc cagtctttgt
     3301 cggctccagc aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc
     3361 gccgctgttc cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt tgcacagcct
     3421 gaatatgctg cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa
     3481 tatttcggta gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac
     3541 cggttcaacc tccactggcg atacgttgct gacgcaatct ctgctgatgg agctttccgc
     3601 actgtgccgg gtggaggtgg aagaaggtct ggcgctggtc gcgttgattg gcaatgacct
     3661 gtcaaaagcc tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg
     3721 catgatttgt tacggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc
     3781 cgagcaggtg gtgcaaaaac tgcatagtaa tttgtttgag taaatactgt atggcctgga
     3841 agctatattt cgggccgtat tgattttctt gtcactatgc tcatcaataa acgagcctgt
     3901 actctgttaa ccagcgtctt tatcggagaa taattgcctt taattttttt atctgcatct
     3961 ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata ttatcagttc
     4021 tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca ccatcatggc
     4081 actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac ttaaaacact
     4141 gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa ccaacagcat
     4201 taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga aaaaagcccg
     4261 ccagttaaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta gctggtcagc
     4321 aaatacgtcg acgcgcggca ggcgctgatt agcgcccctt cttttttcgc cccggcgagg
     4381 taaaacgctt accgttggac gccgggcgac cgcctttttc cgccgttttt tccatcttaa
     4441 cgactggacg tttgatgccc gctgttttcg gtttggcctt cgctttaggt tttgcctcgg
     4501 aagaggaatt ttcgatgagc ttaaagaggt cgattaactc atcgtcggtt aaatcgcgcc
     4561 actcccccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt tccagctttt
     4621 tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcagc ccctgcacca
     4681 gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt gcacttttta gtcaccgtcc
     4741 cgaggatcgg cacccccgcc cccataccac gaataaattc atcggtaatc ggtttatcga
     4801 ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta ttcaccagat
     4861 cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg ccaatcggga
     4921 acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca tcttcggtgg
     4981 tgctgacaat gcctacgggc ttgttcaggg cgataagtac caaatcttcg gcttcccgag
     5041 gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg tttcacctga tcgccaatgg
     5101 tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc gcttcgcggc
     5161 gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag tcgggcagca
     5221 taaattctcc tgtaaaacgc ggaatatacc gtacctctta gcggggaaaa acagggttat
     5281 gcggcaacgt cgaaattaaa tgtgatctgc cacgcctttt atcacaaaag tgtctccgga
     5341 agtacaatgc aaatttgcgt ggcaaaataa ggataatcaa catattacga atcgctgcac
     5401 acctgtgctg ataactactg cgcagagaaa caaatgtgca agagaaagcg ctatttctcc
     5461 ccatgcaaag agtgaaaaat cgctttgaaa ggccatcatg atggggttat atcagatcaa
     5521 gcggtgagaa agcgtatttg cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt
     5581 gacgatatcc gtttgattgt gaagattgcc caactctatt acgaacagga tatgacgcag
     5641 gcgcaaatcg cgcgcgaact gggtatttac cgcaccaaca tcagccgctt gcttaaacga
     5701 ggccgcgatc agggaattgt caccatcgcc atcaactatg actacaacga aaatctctgg
     5761 ctggagcagc aactgaagca aaagtttggc ctgaaagacg ttgtggtggt gtcgggaaat
     5821 gatgaggatg aagagactca actggcgatg atggggttac acggcgcgca actgctggat
     5881 cgcttgctgg agcctggcga tattgtcggt ttttcctggg gtcgcgcggt gagcgcactg
     5941 gttgaaaact tgccgcaggc ggggcaatcg cggcagttaa tctgcgtacc aattattggc
     6001 ggaccgtccg gtaaactcga aagccgctat cacgtaaaca cattaaccta cagcgcggca
     6061 gcgaagctga aaggggaatc gcatctcgcg gattttccgg ctctactgga taacccatta
     6121 attcgtaatg ggatcatgca gtctcagcac tttaaaacca tctctgccta ctgggataat
     6181 ctggatgtcg ccctggtggg aattggctca ccggccattc gcgacggcgc taactggcat
     6241 gcgttttatg gtggtgaaga gagtgacgac ctgaatgccc gccaggttgc tggcgatatt
     6301 tgctcgcgct tttttgatat tcacggcgaa atggttgaaa cgaatatgag cgaaaaaaca
     6361 ctctctatcg aaatgaataa attaaagcag gcacgatatt ccattggcat tgccatgagt
     6421 gaagaaaaat acagcggaat tattggtgca ctgcgtggaa aatatattaa ttgtctggta
     6481 acgaatagca gcacagctga actattactg aaataaaaat cttcccgatg tttatcgtaa
     6541 taaaaattac ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta
     6601 aatttgcagg ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt
     6661 gtagaggaat tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat
     6721 ggacaatatg aaggtcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa
     6781 gaggtaaatc atacggtagc ggaaattatc cagcgttttg gtcgcaccga cggtctggtc
     6841 aataacgccg gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag
     6901 tatgaactca acgaagctgc attcgaaaaa atggtcaata tcaaccagaa aggcgttttt
     6961 ctgatgtcgc aggcggtggc gcgacagatg gtcaaacaac atgatggcgt gattgtgaat
     7021 gtttcctcag aaagtgggct ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa
     7081 gccgcgctca atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt
     7141 gtggtcggta tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa
     7201 gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa
     7261 aacgccattc ctattgggcg cgccggaaga ttagcagaaa tcgctgattt tgtttgttat
     7321 ctgctgtctg aacgcgccag ctatatcacc ggagtaacca ctaacattgc gggcggcaaa
     7381 acgcgcggct aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct
     7441 tgcgccatgc tggaatcggt tcagatggtt tacggcaacg ccaatgttga agccgttgaa
     7501 tttgtgcccg gtgaaaacgc cggagacatc gtagcaaagc tggaaaaatt agtaagcatt
     7561 cacaatcaaa atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg
     7621 gcagcgatgc ttgcgatgcg caatcctcgt ctgcgggtaa tcagcggact ttctctgccg
     7681 ctggcgctcg aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac
     7741 ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac aactggcaac gacagaggag
     7801 gatttctgat gaacattacg ctcgcccgca ttgatgaccg cctgatccac ggtcaggtca
     7861 ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt
     7921 ataacgatga agttcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta
     7981 acgtggtcaa tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat caggacgaaa
     8041 ccgtttttta tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa
     8101 aaataggcac cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca
     8161 aagcggtttc attagatgat gatgatatta acgcatttca tgagttaaat aatctaggcg
     8221 tcatactgga tttacgcgtc gtagcgtcag atccctcaat caatattatc tacaaaataa
     8281 acgaacagtt aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg
     8341 gatgacgaca cttaaaaggt gacaaatcat ggaaataagt accctacaaa tcattgctat
     8401 atttcttttt tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg
     8461 cccgttaatt gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat
     8521 actcggtggt acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc
     8581 tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag
     8641 catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg tgctgacggt
     8701 gtttgcccgt accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaagc
     8761 acggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt
     8821 cgccattccg gcactgattg tctcgctgtt cgtcagcgcc gatatggtga gcaatatgtt
     8881 aaacgccatt cccgaatttg tgacccgagg tctgcaaatt gctggcggtt ttatcgtggt
     8941 agtcggttac gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt
     9001 cctcggtttc ctcgcaggtg gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg
     9061 cgtgatcatg gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca
     9121 actcctggcc cccgcttcca ccgcccttga ccaacttgac gattaacgga gccatcatgg
     9181 aacagagaaa aattacacgc ggcgatctgg tgagcatgtt tctgcgctcc aacctgcaac
     9241 aggcgtcctt taactttgaa cgtattcacg ggctgggttt ttgctacgac atgatccccg
     9301 ccatcaagcg actttaccca gtaaaagagg atcaggttgc ggcgctcagg cgacacctgg
     9361 tgttctttaa taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg
     9421 aagaggcgcg ggccaacggc gcggaaattg atgacggcac cattaacggc atcaaagtcg
     9481 gtctgatggg accattggca ggggttggcg atccactggt ctggggaacg ctgcgcccga
     9541 ttaccgccgc gctcggcgca tcgctggcgc tgtctggcaa cattctcggc ccgctgctgt
     9601 tcttctttat tttcaacgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct
     9661 ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg
     9721 aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa
     9781 tcaacgtacc gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcaccatga
     9841 ccgtgcagaa cattctcgat caactttgcc ctggtttgct ggcgctcggt ctgacgctac
     9901 tgatggttcg tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg
     9961 gcttagggat tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac
    10021 tgccggggcc atcgttcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg
    10081 gtaaacgtga tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc
    10141 tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg
    10201 aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc ggccacgagt
    10261 tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc
    10321 gttatgtgat tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc ggctactcct
    10381 tcccgtgggt aggcggcgag gccacgcatg tggttattcc caacgaggtc atggaacaag
    10441 attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt
    10501 cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca
    10561 cgatggggat tcgcccgcaa gggcgcatgc tgatcctcgg cggcaccgga ccaatgggac
    10621 tgttggcgat tgattatgcg ctacatggac ccgttaaccc gtcgctgctc gtcattaccg
    10681 ataccgacaa cgataaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac
    10741 tgattcatta tctcaatgcc gccgatgcag catttgatac gctaatggcg ctgagtggcg
    10801 gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct
    10861 cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt
    10921 tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt
    10981 caggcggcaa taccgacgac atgcgcgcag cggtcaaatt gattgaagag aaaaaagtgc
    11041 aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg
    11101 aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga
    11161 cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt catcagggga
    11221 tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt tcccatgatt
    11281 aatcgcgcta cttaactctg catgtcgctg gctggtcacc gcggcaactt cgcgacctga
    11341 tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc
    11401 cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaataaa
    11461 gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct ggtaggcctg
    11521 gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata
    11581 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgccttccgt
    11641 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
    11701 ggtaaagcct ggctggaaca tgcactgccg ctgattgctg aacagttgca gggtcgccgc
    11761 tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta cacagcgaaa
    11821 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
    11881 cccgttgccg cgattgaaaa tgcggaaatt gtgattgtcg gcggcggtaa tactttccag
    11941 ttgctgaaac agtgccgcga gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt
    12001 ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
    12061 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg
    12121 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
    12181 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
    12241 ggtaactgga tcacagtgag taaaggtcac gcaacgctgg gtggcccgaa taccacctat
    12301 gtgtttaagg ccggtgaaga agcggttccg ctggaagctg gtcaccgttt ttaagtgaat
    12361 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
    12421 atcaggccta caggattcac aatattaata ctcgtcccgt ccctcatctt catcaggctg
    12481 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag
    12541 taagcctaaa tgtagcgaac tggtttcaat gctttgcacg ttttgctgat gcaggcgatc
    12601 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa
    12661 acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact
    12721 gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa atgctcgcct
    12781 tgcagccagc gatttgtcgg caatttcact actcatgcgc tcgacgatat cggaggcctg
    12841 ttcaaggttg agcgacattt cgatgatctc cgcccagcgg cgcgactctt cttctgccag
    12901 ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa cgttgatatc
    12961 atctgccagc ttacgcagct ctttctcctg ccttggctcg ccgtgcatca ctttattcag
    13021 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
    13081 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
    13141 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
    13201 tggcagcatg gccaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
    13261 cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat
    13321 aatcaggcta cccaccagct taaacagcag actacccagc gcgacacggc gggctgcggc
    13381 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac
    13441 cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag
    13501 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
    13561 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
    13621 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
    13681 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
    13741 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
    13801 cccgacatct gcccccagca caatgaccag agccggtgcg agggctacca gatcctgagc
    13861 gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc
    13921 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
    13981 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
    14041 ggcgacggca gaaagcaggt taagcagcgt taacacgagt tcaggttctc cttatcgtta
    14101 tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac
    14161 tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt
    14221 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
    14281 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
    14341 atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg
    14401 cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc
    14461 gggccacatg gcgaaagatt ctgtgagttt catgccggta tgtttttcca cttccagcag
    14521 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg
    14581 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
    14641 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg
    14701 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
    14761 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
    14821 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
    14881 ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta
    14941 gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc cacgcggatt
    15001 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
    15061 cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca
    15121 ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac
    15181 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa
    15241 gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg
    15301 ctgaatacgt acggtttcgt actccttgcg ggtacgagcg acaaaatcat cgcgctgagt
    15361 atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt
    15421 cgggccgctg tagttctgct cgattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc
    15481 accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat
    15541 ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt
    15601 agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt
    15661 gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac
    15721 ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg
    15781 ttcgaggtag gccactgcct gcttcatcac acgcgccgat ttgaccacct gtggcaggaa
    15841 cattttccct tcgccgaaca gatcgccgac cacgttcatg ccgtccatta gcgggccttc
    15901 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa
    15961 ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca
    16021 ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag
    16081 ctccagtaaa cgctcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc
    16141 gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat
    16201 atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg
    16261 atcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg
    16321 cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc
    16381 ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa
    16441 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcaattt tccgtgcgcg
    16501 ggtatcggcc tgcccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg
    16561 caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac
    16621 aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc
    16681 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
    16741 catcgccgct tcggcatcga gcatcccttc atccatgttg atatcgataa tctgcgcgcc
    16801 gttttccacc tgctgacgcg ctacatccag cgcctcgctg tatttctctt ctttgatcag
    16861 gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct
    16921 atcgtcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg gaatttcagg
    16981 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt
    17041 ggtgccacag cagccgccga cgatattgag aaaacccgct tgcgcccatt cacgtatctg
    17101 ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt
    17161 cgggtgcgcg gtgacgtagc attccgcaat ccgtgatagt tcctgcacgt actggcgcag
    17221 ttcatctggc cccagcgcgc agttgaggcc aaaagtcagg gcttcggcat ggcgcaggga
    17281 gttgtaaaat gcttcggtgg tctgtccgga gagcgtgcgc ccggaggcgt cggtgatggt
    17341 gccggagatc ataatcggca gctcaacgcc taacgcttcg aactccgttt tcaccgcaaa
    17401 gaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcagaatca gatccgcacc
    17461 gccttccact aatgctttgg tggattcgcg ataggccgcc accagctggt caaaagtgat
    17521 gttgcggaat gccggatcgt tcacgtccgg agaaatggac gccgtgcggt tggttgggcc
    17581 gagaacgccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca
    17641 ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
    17701 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
    17761 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttgcttagca ccagcagatc
    17821 gttattgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
    17881 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc
    17941 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa
    18001 cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg
    18061 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
    18121 gcattagcta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat
    18181 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
    18241 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
    18301 tggcagtgtg gcactcacgg agctggcgca acaagccggg ttacccaatt ccacgaccca
    18361 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca
    18421 ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
    18481 gttagcaatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa
    18541 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg tacagtgtac
    18601 gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc
    18661 gggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
    18721 agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct
    18781 cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcgc tggggctacg
    18841 ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc
    18901 cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa
    18961 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
    19021 aggcaatatt ctgcccgtca tacctgagtg gtaatagaat aagggtgtct gttaatcgca
    19081 ttgacgtcaa aataacttat tgtcatacac ttcatacact tttattaact cagcattatt
    19141 tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt
    19201 ttacggctgc atta
//
LOCUS       DAZGXS010000088        19022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGXS010000088 DAZGXS010000000
VERSION     DAZGXS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..256)
                     /locus_tag="ACHKG5_003203"
     CDS             complement(<1..256)
                     /locus_tag="ACHKG5_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869254.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYIL
                     SIIYPIKLNVGDDEFKFIFYIMSQKKFLRQQTISDFVRSIN"
     gene            complement(402..4304)
                     /gene="hrpA"
                     /locus_tag="ACHKG5_003204"
     CDS             complement(402..4304)
                     /gene="hrpA"
                     /locus_tag="ACHKG5_003204"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0869255.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4505..5110
                     /gene="azoR"
                     /locus_tag="ACHKG5_003205"
     CDS             4505..5110
                     /gene="azoR"
                     /locus_tag="ACHKG5_003205"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0869256.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5161..6453)
                     /gene="ynbD"
                     /locus_tag="ACHKG5_003206"
     CDS             complement(5161..6453)
                     /gene="ynbD"
                     /locus_tag="ACHKG5_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP0869257.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6467..8114)
                     /gene="ynbC"
                     /locus_tag="ACHKG5_003207"
                     /pseudo
     CDS             complement(6467..8114)
                     /gene="ynbC"
                     /locus_tag="ACHKG5_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            complement(8130..9026)
                     /gene="ynbB"
                     /locus_tag="ACHKG5_003208"
     CDS             complement(8130..9026)
                     /gene="ynbB"
                     /locus_tag="ACHKG5_003208"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0869258.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9026..9631)
                     /gene="ynbA"
                     /locus_tag="ACHKG5_003209"
     CDS             complement(9026..9631)
                     /gene="ynbA"
                     /locus_tag="ACHKG5_003209"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0869259.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9802..12108)
                     /gene="ydbD"
                     /locus_tag="ACHKG5_003210"
     CDS             complement(9802..12108)
                     /gene="ydbD"
                     /locus_tag="ACHKG5_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP0869260.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYEIS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12172..13032)
                     /gene="pdxI"
                     /locus_tag="ACHKG5_003211"
     CDS             complement(12172..13032)
                     /gene="pdxI"
                     /locus_tag="ACHKG5_003211"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0869261.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13252..15711)
                     /locus_tag="ACHKG5_003212"
     CDS             complement(13252..15711)
                     /locus_tag="ACHKG5_003212"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0869262.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15931..18909)
                     /locus_tag="ACHKG5_003213"
     CDS             complement(15931..18909)
                     /locus_tag="ACHKG5_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFP0869263.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPYFFNPSTGVNISGDNND
                     VTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVGEKNVMD
                     DGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGAELEIGA
                     DATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDSTVINNG
                     SIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLSDGTSFN
                     NKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLDTLWVDE
                     NDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAGAGMAAY
                     GNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININAENGQAF
                     YNDGTGLIINYGTICTFGVC"
BASE COUNT         4810 a         5094 c         4287 g         4831 t
ORIGIN      
        1 catttattga gcgtacaaaa tcgctaatgg tctgttggcg tagaaatttt ttctgtgaca
       61 tgatgtaaaa aataaactta aattcgtcgt caccgacgtt aagtttaatc ggataaatta
      121 ttgataaaat atacagcgcg cgtcggtaat tttcctctgg aagtttttca tagaaagata
      181 ttaaagcctg accaacgcga tcaccctcta ttatccgttc aaattgcgac ctcagtaaaa
      241 tatcatgtct actcattgca atacgtcctg ttcgcccaag gcgaagtatg aatgaaactt
      301 atgtaaaata gtaccgccct tgctatattt cagaattcat aaagaaaaaa cccggcgcta
      361 aatgcttacc gggttttaga tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      421 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
      481 taactaacgc gcagctcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
      541 cgtgcaggcg ggagtttgtt gaaccattgc tgccacgcct gctggacgtt ttcgactttc
      601 agcatctgtg cacgatcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
      661 gcctgcaaat aacgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
      721 cgatatacca acccgcccat ctgcgcttta atgtcagaaa gccccagcgc catggtcata
      781 tccacccgcc ctttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
      841 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
      901 gcaaagcctt cttccgtcca gaccgggcct ccattggcgt cgatcagttg atccacaccg
      961 catgagatac agtcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1021 agcttggctt tgtttggtaa cttctcatgt aaatacttga ttggtgaggg aatattcagc
     1081 agcagtagac ggcgaagacc gttccacatt gcctgttttt gctccagcgg gttatcaaac
     1141 agtttgatcg ccacactgtc gcgctcatcc accagcgccg gccacgcctt cactttgtag
     1201 ttaccacgct tctgttcgta gctttccggc aactgaccaa aactccagat atgtaagccg
     1261 ctctgctcga taccgtcatc cgccaccgca gatagcgttt cctgcacttt gcctttcagc
     1321 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1381 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1441 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     1501 agcggtgtga cgcggcctaa aaacgcttcg gcatagtttg gcgcaggtac aaaattacgg
     1561 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca gttcgcggcg cagaccaggg
     1621 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     1681 gtcacaccgt cagcatccgc gccgggctca aactgatagc tcaaacgcag cttgagattg
     1741 ccctgatgcc agaagttcgg gtaatccagc ttgctgatct tttccgcccc ttctttgatc
     1801 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     1861 ctgtcgaagt gacgagcgga gattacatcg tggccgatgc gctggtcgta gaactcaaac
     1921 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttcaag ctcttccact
     1981 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2041 accagcgcgt ggcgaataaa gagttcacga cataacgccg gatcgatctg gctgtagttg
     2101 accttgcgcg cggcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2161 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtac gtttaatcaa atgctgagcg
     2221 acaggctcca cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2281 tctaccagtt ccgccaccat tacccatttc ggcggttttt tgaataaacc agaaccgggg
     2341 aagatggaga aacgcgcgtt acgtgcgccg gtatattctt gtttatcggc atctttcatg
     2401 ccgatatggg aaagcaaccc cgtcagcaac gcaatgtgaa tttcacgata ctccgccggt
     2461 tcgctgttca ccggaatgcc gagttctttc accacctggc gcaactgggt gtagatatcc
     2521 tgccattcgc gcacacgcag atagttgaga taatcggtac gacacaggcg acggaaggcg
     2581 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagattcac aaacgcgagg
     2641 aagtcagact ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     2701 atcggacgtt cgcgcggatc ctgaatggag agcgcagacg tgataatcat cgcctcacgc
     2761 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     2821 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     2881 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     2941 tcgacaaacg ggaacgcagc gatatcgcct agccccagcg cggtcatctg caaaataacc
     3001 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3061 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3121 tgattggcag acgcctgcga aatgggctca atcggcaaac gctgcacttt ggtgcgatag
     3181 ctgtagcggc tgatacgcgc cgtaccgggg tcgataacgt atttaatccc cggtacagtc
     3241 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgtc gcccgctgtg cgactggaac
     3301 acgcggttct gttcgctatt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3361 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcgcc gctcataaag
     3421 atcagaatgt cgccggggct ttcctggctt agttcgtcca cggcatcgaa aattgcctgc
     3481 aactggtcgc gctcagtgtc gtcggcttct tcaacaatcg ggcgatagcg cacttccacc
     3541 ggataggtcc gaccggagac ttcaataatc ggcgcattat taaagtggcg cgaaaagcgt
     3601 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     3661 tctttcaaat aaccgagcag aaaatcgata ttcaggctac gttcgtgcgc ttcgtcaata
     3721 atgatagtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     3781 tcggtcatta gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     3841 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc taccgttctt
     3901 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     3961 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gactatcacc
     4021 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggcaaattg
     4081 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4141 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4201 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4261 cgctgctgca aggccgtaaa agtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4321 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc gcggctttct
     4381 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4441 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaatatc tataaggaaa
     4501 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     4561 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     4621 cgttcgcgac ctggctgcaa atccaattcc ggtactggat ggcgaactgg ttggcgctct
     4681 gcgtccgagc gatgcgccgc tgactacgcg tcagcaggaa gctctggcac tttccgatga
     4741 gctgattgcc gagctgaaag cccatgacgt tatcgttatt gcggcaccga tgtataactt
     4801 caacatctcg actcagctga aaaactattt tgacctggtt gcacgtgcag gcgttacttt
     4861 ccgctatacc gagaatggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     4921 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     4981 gttcctgggc tttatcggca ttaccgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5041 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagtattgt
     5101 tgctgcataa ccggacacga tcttgatgtg gactgtagtt cagtccacat ctcaatccac
     5161 ttacctgttt tcccataatt tcaacatcgc tttgtgctct tccttaagca caatatgcga
     5221 gcgtctggct cgaataaagc taatcgcttc atcaactgtt ttacagtgtc cgtaacataa
     5281 cagccatgcc gccactacca gcgcactgcg cgataatccc aatgcgcaat ggaccagaac
     5341 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5401 ttccggaacc accagatcca gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     5461 tccgcgaggg aattcaaagg tgacatccag aaccgcattc tgtgccggaa tatgtcgtgg
     5521 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     5581 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     5641 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     5701 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     5761 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     5821 tattttgatt cgacgttgat caggtttctg ataattccaa cgacggtcga cgggtatcat
     5881 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     5941 ggttgtcagc atcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6001 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaacaa tatgcagcga
     6061 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcctgtcac
     6121 cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa aaccgcagca
     6181 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgagct
     6241 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggga caatagtcca
     6301 gggaagaaaa gggatcgccg tttcccagcc gaatacctga ctggggatat catggctgtt
     6361 aaagtcctga accgcggtga actgatttag agatccatag gtgaagaaga aaaacggggc
     6421 cagcaacaat aaccagccag cgccttgtag caatacgttc cggcgttcag ttatcacgac
     6481 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     6541 atccggcatc acgcacgagt gaatccatct ccccttgcga acgcacgcgc attacccacg
     6601 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     6661 gctgcccggt gtagaggaga atgccgcccg gttcgatggc attggcaaga cctgcgagtg
     6721 agttttttac ctgctcattt ccgggaaaaa gctcatacag tccagagaca atcgccagcg
     6781 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     6841 gcacccgccc agacattccc cgttgagcaa tcatctcttg cccctgtgca acatttaact
     6901 cgctgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc gcatccagta
     6961 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttggcgtgga
     7021 gatcggcaac ggcttgttta atcagtattt gtaaatgggt tttgcgctgg cgaataccgc
     7081 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactacctt
     7141 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagccg gtgctcattc
     7201 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7261 acgccaaatc aaccggcgat aatgctttgc attttttcaa atgcctgcgc cctgttctct
     7321 tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     7381 aatctatgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc
     7441 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     7501 taaagatcga gcaagatatt aacggcaatc gcccgtgtga tcagcggatc attattgaaa
     7561 ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga attaataaaa
     7621 aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     7681 ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga
     7741 acccatgtgg caaccagcac tgcgccgacg ctctgcgcga tcactaccac ctcttccagc
     7801 ccgacctggc tgtcgctggc agcaaaacgg acaaactcat cgacatcccg cactgaacgc
     7861 gcaagagatg ggctataacc acggggcccc gaagtttgtc catgccctcg ggcatcccat
     7921 gcataaaaag cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa
     7981 tgttcatgac cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc
     8041 cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     8101 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     8161 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctggt
     8221 aatagttttc cgctatcttt aacacccaca tctcgtttga cggctgacat cactacatcg
     8281 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     8341 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccacc
     8401 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     8461 ttcccccata aatactgtgc aatatcgttt gactctgtta acgccaaaag aaataacacc
     8521 agtaacgccc cgccctgggt atttgtggtt gtcaacatta ataaccaggc ggcatgactc
     8581 aaggcgaaaa cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg
     8641 gaggtatcac ctactaaaac tcgccaggtg gttaatatca gaaacccagc caaaggtata
     8701 aataacagaa acagctcaaa gcaactaaaa ccgataagca aatagttaag aggaataacc
     8761 caatataacc aacgcggaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     8821 aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat caccagtgaa
     8881 aaacaaatga cgatacccca ccaggtacga atacgtagtg taacttcatc cccttttctc
     8941 tccggtaacc gccatacccg aaaacggttt atcagcgttg ctaaaattaa caacgcgaaa
     9001 agtgttgcca gagatttttc cagcattatc tttcagcact catcaggagc acactccgac
     9061 aacgattaat cgcagtccag agaagcagaa ttgaagcaat gctccataaa agattattcc
     9121 attgcatcca ctgtggataa atggcaacgg ccagccccca caacccaaat attaacgcgc
     9181 gatcgctttt gccaaaagga ccgacataac tacgaatacc gttaatcgtc tgcgcgagta
     9241 aaccgcaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt gatgcgttac
     9301 tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca tcgccagttt
     9361 catttaaaat cgcccccaga cgtgtttgct gattgcactc acgcgccaac atgccatcca
     9421 gcgcattgag tgccatacgg ataaaaagca cgatgggcaa tagcaaaaag aggatgggtt
     9481 gtgcgaccaa catcaacagc aatccggtaa taagagaaag cgccagtgca gcaagagtga
     9541 tgtgattcgc tgtaacatgg tgcttataaa gccaaaacat cgtcggcctt aacagcgact
     9601 gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc ggtttgcgct
     9661 atggtgcatc aattcccctg acggagatat cgcaacacga cgcttcatta aaaattcaga
     9721 atatatcaag gtattgggga ctcactgtca ggaatgatta tccccaataa atcaagtggt
     9781 taaaaattat tacgctgtta ttcaatacaa ctcatcaagt tttctcatcg cttgttcccg
     9841 tattgcgcgc gtcttaccgt taagcgccag ctggcgaact aaatccagtt cagggttaac
     9901 aagctttaac gccagcaaag gatcacgttt cagcctcgcc tttaatacat caactggaaa
     9961 acggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg ccagtactgc
    10021 aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat tgcttttcgc
    10081 cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt gtgctaacgc
    10141 atcggggtct agtgcataat aaacacgtaa cgcttcgtta acctgcgcac gggaactgtt
    10201 ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtgtttt gctgtaattc
    10261 cttgagaaca acagcgggta aattatccca actaattaat gcactctcta aaaagtccca
    10321 gtcgtccgaa cgtatcagag aggtggcatt taatagcatc tggtcatcaa cttcaccgta
    10381 gctgataagc tcagacaaaa tcatatcagc gctgatttca tatgcatctg aatctgcttt
    10441 gggattattt ttccgatgcg ataaagcacg ttgccaaatt ttttgtttca tcgacgtgct
    10501 cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac gtgtcaataa
    10561 ccgttgtaaa gcagcgtcag agtgaattat ctgcatatcc cgacagcaaa agtaggcagg
    10621 ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca acgctaaaaa
    10681 taacgcacct attagatcat cgtcattctc gaatttttgg tataaatata ctgctatttt
    10741 gtcactctct tgtggcgtca tccgcaacaa ttgaccatgg tgtttcatct ctgccagctt
    10801 taacgctaag cttgtgacaa catcgtttga tgaatcgttc gccaactgca ttgcctgatt
    10861 gtaggtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac tgcggatata
    10921 gccatcaata atggattcgg gcaataacca cgcatcttta taaagctcct gaatgatcat
    10981 atcccaatct cttttcgcca gagtcatgac gatatccaga gggagatttt cacgtagcgc
    11041 aagattcaac ggaccgacac catcaggtag atgactcatt cgcgtcatta tttcgacaga
    11101 gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt gctgatcggt
    11161 caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg tttgtccatc
    11221 attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc ttgggagagt
    11281 tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag caacgttgcc
    11341 ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttaaggc gaaacagcgc
    11401 ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc gcttaaaaat
    11461 ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag gatttattat
    11521 catcagcgct tttttgacat tttcagtgtc atttttgtca ctcgcacgga ccatgaattc
    11581 actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata tcccacgcgc
    11641 attaatactc cccttaatcg tagacggatt atcgtaatat gagtcgataa ataccagtcc
    11701 acttgcattt atcgaaccgg taattaacag tggtgaatcc gtaactatat tcgttgccgt
    11761 cacgttaccg agaataaccc atggattaga aagagagaaa gtggaaatat tacctttgac
    11821 tgtcaaattg ccaagaacaa tcaatttttg attactctct atattacctt ctaccactaa
    11881 atcgccacga tgcactttcc agtccgatgc agataaaccg aaggggacaa gattcacgct
    11941 actgttgata gcttgttcaa cttgttgttt atcttcagcg gtagccgcag aaaaaaaagg
    12001 ttcaatatca actgcatttg caacgcatcg aagttccgcc tgtactggaa gactcagcat
    12061 aaacagggga agtaaaccta tccagctgca aagcctgggc ttaaacatat gcactccatg
    12121 ccattaaaaa agaaacaaaa gggaaatgca tctttccctt ttgtatattc gttattctcg
    12181 cgaaatacca tccaacgtag acaacacttt ctcagaaaga tgcaactttt cagcagccat
    12241 attctcccgt aaatgcgcaa cagaagacgt ccctgggatc agcaaaatat tcggtgaacg
    12301 ttgtaacagc cacgccagcg ccacctgcat tggtgttgca cccaagctcg cagcaacatc
    12361 ggaaagcgtg gaagattgca gcggcgtaaa gcccccgagc ggaaagaacg gcacgtaggc
    12421 aatgccgtca cgagccaaag cttcaatcat tgcgtcatca gcacgatgag caatgttgta
    12481 ttcgttctgc acacagacga tttcggcaat cttgcgcgcc tctgcaacct gcgtcggtgt
    12541 gacgttgctt aggccaatat gttttaccag gccttgttgt tgcatctctg ccagcacggt
    12601 caggctggct tcaatcgatc cttccgccgg gccatgacca tcccccatca tcacgcgcag
    12661 gttaaccaca tccagtacgt ccaggccgag attacgtaga ttatcgtgca ccgctttttg
    12721 cagctctgcc ggagaaaatg cgggcaacca ggatgcgtcc tctccacgcc gcgcaccaat
    12781 tttagtgaca attgtcagat cgtcagagta aggataaagc gcttcgcgga taatctgatt
    12841 ggtgacgtgc ggaccataaa agtcgctggt atcaatgtga ttgacgccca atgccagcgc
    12901 ctcacgcagc acggttatag cgacgtgacg atctcttggg gggccaaaaa ccccaggacc
    12961 tgccagttgc atcgcgccat aaccaagacg gttaacggat tttgtaccga gagtaaatgt
    13021 attgctgctc atcgcaccct gccctgtaat aaaacggatt aagaaaaatc agtatagaaa
    13081 cgaaaagaga taaataatgc gaaggcgtaa acaagatttc tggatgagta gcactattgt
    13141 cctgaaagac accaaaacat tagacagcaa aagataagtc aagcgtcgca tccggcaata
    13201 aaaaaccgca acctgtatca gcgttgcggt ttgtgtatca catgctaaaa attagaacgt
    13261 tttcttaaag ttcagcatca cgcctttgtc gctgatgccg tcctctttcc agtgatacgc
    13321 atccagattc agcgaacttt ctttgctact gtacttcacg ccgacgctcg ccaggctgtt
    13381 gataccgccg cccttctgac cgtcatcgac gttaaagtgc tggctgcctg cccctgcaag
    13441 gcttgccgtg cgctggctct tgctgtagct caggttcggc ccgccttcca gcgtcgcatt
    13501 cgcgctccag ccgcctttcc ctgcgtagtc cagtttcagc ccgacgatgc tgtccaccgc
    13561 cgtttcgctg ccgctgttca tgctcaggtt aaagtctccg gcattgcgct cctgatagcc
    13621 gccttccagc gagtgacgca gtttcactcc ggcgtacggg gtgattttca gcccttcgcg
    13681 cggctcaaag gttttcgccc cttcgctacg caactccagg tactgctgtt tcacatccgt
    13741 atccgccgtt ttgctgacgt cgccgtaagc gaccgagcgg ctgctgtcga gattatgcac
    13801 gtcataacgc agcgcgttgt tccaacgcat accattgtcg aaggacatct ggtgcttcag
    13861 gccaaagaac tggctgtagc cgccggttac gccattgtcg cccgctttct gcgcaccgtc
    13921 gccatccaga cgcgcgatac cgtattccag gctcatgctc tggctttcgc tcaggtcaac
    13981 ggttttacgc agtgccagca tgtcatactc ggtgttattt ccgagttccg cacgcgggtc
    14041 acctttcgcc accacgttaa aggccaggcc attgcccacc ttcggcgcgg catccgccag
    14101 catgctgaag cggttgctta acacacgcgc ttcacggaat accgtggtcg cctggctacc
    14161 gctcacctgc ttcagggcgc tattcagttc agcagtggtg cccacgttca ggctggtata
    14221 cagctcgtta ttggtgtaac ccgcatccag tgccttcgcc acatcgttca ccgaagcatc
    14281 ggtcgccaca tcggtgtagg cgtttttgct catgatgacg tcaacgttgc cgctggcatc
    14341 ggtgctgcct ttggcggtcc ataccacgga cgttgaggtg atggcatcgg cgtcggtcag
    14401 gttgatgcct tccaccacgt tgtcgaaact gaccgtggtg tctgccgtac cggcggcgaa
    14461 accggtgttg atcccgacac cattcatgct ggcgttgttg accttcagtt taccggcgct
    14521 gccgtcaacg ttggtaccga cgatatagcc gttgaggttg ttactgctgc catcgctact
    14581 accgctatca gtcgtttcac tgaaaggtga agtggtgtaa gtgtttggca tgtccggcaa
    14641 cgtgtagtcg gtgtaatgca cttcggtatt gttaccgctg ttgccgttca ccccttccac
    14701 attcacgctg tcgccctgct taatcaaccc cgaaccagtc acgccgtcgg caatcaccac
    14761 cgtaccgttg ttaacgatat gaccttctgt gcccagtacg ctgaacgcaa acgagtcgtt
    14821 agcaaaaata ttgatcgtgc cgttgttctc aattaccgca tcggcggtgg cattggcatc
    14881 cagttgcata ccagtcaatc cggtatcggt ggtgccttcg gtaccgagat tgataacgcc
    14941 gttgttaacc agctgtgcgt ttttatcgcc ttccatcgcc acggcgttgt tgccgctgat
    15001 gttcattaca ccatcgttga gagcaacata gttgttgtgg gtgaatttaa ccgcgctggc
    15061 gttgtcttta tcgaagttaa ttaccccgcc atcctggttc cacaagaagg catcgctttc
    15121 tgtcgcaccg gcagtactta ccgccacggt gactttcgca tccaccacgc ccttgttaat
    15181 aaactcgctg gcgccatcgt cgtagtgaat agcgcccgca cctaatgcgg catccagcgt
    15241 catttcaccg gtgttatata acgtggcgtt agcattgctg ccctggaagc gaatggcgtt
    15301 tttgctgtta tcaaggatga gtttgccgct gttgttgaac gttgacttat cgtcaatatc
    15361 cagcgtcccg gcattgttgt tttcatcctg ataacccatt ttccaggtac ccgcgttatt
    15421 cactaccgca gagttataga gaatcgcgcc tttacgcgcc tcgccgcctg caaggttatt
    15481 gaataccgca tcgccctgag cgttaacgcg cgccgacgca ataccgctgt tattcaccgt
    15541 accatcgtga agttgtaaag ttgacaggga accggtgtta ttaattaccg cgttgctgtt
    15601 tttctcaatt tttacgacgt cattaagcgt tccgttgtta ttgaaggttc ctccttccag
    15661 agtcaccgtg ttcaatacac cttcattggt taacgcaccg ccttcattca cgacgatggc
    15721 tgagctaatg gttccgctcg actcgttaac cagcgcacca ttttcgagta tcctgatcgt
    15781 tccattttta acagcacccg cactggtgac atccccgttg ataatcgcac ctgacgataa
    15841 taatacggtc tcacccgcag aacttaatac agcgccatca tcatataaat cagcaatata
    15901 attatccgtt ggattatatt catcggcccc ctagcaaacg ccaaaggtac aaattgtgcc
    15961 atagttgata ataagcccag tgccatcgtt atagaatgcc tgaccatttt ccgcgttaat
    16021 gttgattacg cctgtctggt cattaatggc ggtgccttcg tgataaaccg ccatgccgac
    16081 gagtttattc acaccgagag aatcgttgta attttcattt ttctccaggt tgatggttcc
    16141 ctggttaatg accgtattag tattcccata cgccgccatc cccgcaccgg cgttgtagac
    16201 agtaatcaca ccattctcgt ggttggttgc cgtggcttta gttaatgccc cattataggc
    16261 atcggtacct gaagccattc ccacaccgaa atctttcgcc gtgctatttg caacattatt
    16321 acgcagcgat gtttcatcat tttcatcaac ccacagcgta tccagggtga tttgcccttc
    16381 gttatccgcg gttgatttat cgatggctag catccctaca ctgccacggc catacatatc
    16441 aataataccc gtttcgccgt tcagaatgga tgacgagtag ctggcaagca tacctgtcag
    16501 ccctaatgcc ttgttattga aagaggtacc atcagacaag ttccactctg ccggataatt
    16561 cagcacactg tgctgattca tcattttggt ggtcatggta ccattgttaa cgccaatccc
    16621 accgtattca acatcaaaga cttttggtgc cgggtcggta ccaaaatcgt acatcgccag
    16681 gccaatggat ccgttattga ttaccgttga atctttattc caggcagccg caaaaccgat
    16741 atttttcagt tccacttcac cgttgtttgt cgccttcgca ccagaatcaa tactaatggt
    16801 ggataacgct acacgataat aataagtgaa agaatccaca tcactcatat caaacgttgc
    16861 gtcagcgcca atctccagct ccgcgccatg gctcaactga atgagatttt cgaaccccag
    16921 cgggaactcg ccgctgatgg ttgactcacc attcaggtag accggggaat gcccatcaac
    16981 gttaataagc ggtgtgtctc cgataccacg tcgagaagct gatggttttg agccatcatc
    17041 catgacattt ttttcaccga caaaatggac accgccatta agcgtaaagg tattgtcatc
    17101 gccagcaata acaatacctg caatgtgttc ctgagcaccg tcgtaactcg cataggaacg
    17161 cgacgtggtt ttactatcag caacaacggt taactgacca tccagcgtga cgtcgttgtt
    17221 gtcaccgctg atatttaccc cggttgaggg attaaagaag taaggctgcg cattatcggc
    17281 tttctgatct ttatcaacca gaatactgcc cgctatttcg atagtattac catcacccga
    17341 aacattaaca cccgtcgctt tttgccctgt cacattcagc tcataagtcg ccagcgtcat
    17401 gttgttatta ttgccagaga catcaagacc ggtggagaaa tccccaacgt ctaacccacc
    17461 cgcgagtgaa atattagctt tatccccagc aacgctgaca ccggttccgg cattggtcgc
    17521 ggaaatatca ccaacgttaa caaatgtcgc attatcaccg tcgatctgaa tgccaatggc
    17581 attttcaccc gctgcggcct gaccaacatt gaggtcgccc atgttgatca cgctgacgcc
    17641 atcaccatca agcagcatgc caatagagtt atcttcgatg accgtaatat tacctttgtt
    17701 gctaacgaac gtatcattgc cgtcaacgtc aataccgtgc gcaccaccgc ttacgtataa
    17761 gtcgccttcc tgtttgatgg tggcaccgtc accgacaatt ttcgagccag tggaattctc
    17821 tccgtcaacg ctcatggtac cttgattatc gacccgcgca ttatcgccat tgataaggct
    17881 acccgtacca ccattggtga tggtcgagtc gcccgcagtg tttagcttgg tattatcgcc
    17941 attgatgatt accgcggtac cgccatcaac ttccgtctcg ccattattgt taatggtgat
    18001 attgtcgcca gtgagtatac cgacaaccga gccttctccg atggccgtcg ttttgccgtc
    18061 gttgttgagg gagatgttgt caccatcaac cgtcatgaca gtaccgccat tcagcgccgt
    18121 gttgccgccc tgatcggtga tgatcgcgtt accaaactca atcgcattcc cggttgtgcc
    18181 gtcaacaacg acgccggcct ctttagtgat gtggatgagt ccatcatcgt cgtaactcca
    18241 ggtgatgcca cctgaggtat ttacgccatc aaagacaacg gtgttgctgt cgtcatggac
    18301 tgtccaggac ttaactatcg tctccttgcc ctcaggcgcc gttaaggtgt agctcccatc
    18361 ggcattttgg ctgtaggtaa aggtagtttc gcgaattttg acagtttttg catcctgatc
    18421 ccaggttacg ccatttttat agacgacagg attgtgttta accggggtat cgccaccatc
    18481 gtctggcgga actggcgtat caccgccgtc atctggcgga accggcgtat caccgccgtc
    18541 atcaggcgga actggcgtat caccgccgtc gtctggcgga acaggcgtat caccgccatc
    18601 atccggaact ggggagttat tatggtggtg attgtcatcg tcacttaagg tatatatagc
    18661 aattcccgta gctactgcag caagacctgc agctgcccat ggtaaaactg cactatcttt
    18721 attttctctg cactccgcgg ataaattttc tagctgctct ttgctcaggg aatgaatatt
    18781 tgccggacat gttatcttac tggacttacg tgttttttcg gttgagtcgg gtattgaagt
    18841 atccgcagcc catgtctggc tacttaacgc catggcaaca caaacagaca ataatttttt
    18901 cctttgcata taaaattcct tagatctaga ccgtgcgtaa caagccatgc agaatttgaa
    18961 gataaatcgt agtaaatatt catagtgaaa taagcagtaa agaaatgctt atataattca
    19021 ca
//
LOCUS       DAZGXS010000089        18684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGXS010000089 DAZGXS010000000
VERSION     DAZGXS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1646)
                     /gene="actP"
                     /locus_tag="ACHKG5_003214"
     CDS             complement(<1..1646)
                     /gene="actP"
                     /locus_tag="ACHKG5_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0869264.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRA"
     gene            complement(1643..1957)
                     /gene="yjcH"
                     /locus_tag="ACHKG5_003215"
     CDS             complement(1643..1957)
                     /gene="yjcH"
                     /locus_tag="ACHKG5_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0869265.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2158..4116)
                     /gene="acs"
                     /locus_tag="ACHKG5_003216"
     CDS             complement(2158..4116)
                     /gene="acs"
                     /locus_tag="ACHKG5_003216"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0869266.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4509..5945
                     /gene="nrfA"
                     /locus_tag="ACHKG5_003217"
     CDS             4509..5945
                     /gene="nrfA"
                     /locus_tag="ACHKG5_003217"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0869267.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDAQAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5990..6556
                     /gene="nrfB"
                     /locus_tag="ACHKG5_003218"
     CDS             5990..6556
                     /gene="nrfB"
                     /locus_tag="ACHKG5_003218"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0869268.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6553..7224
                     /gene="nrfC"
                     /locus_tag="ACHKG5_003219"
     CDS             6553..7224
                     /gene="nrfC"
                     /locus_tag="ACHKG5_003219"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0869269.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7221..8177
                     /gene="nrfD"
                     /locus_tag="ACHKG5_003220"
     CDS             7221..8177
                     /gene="nrfD"
                     /locus_tag="ACHKG5_003220"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0869270.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8197..9915
                     /gene="nrfE"
                     /locus_tag="ACHKG5_003221"
     CDS             8197..9915
                     /gene="nrfE"
                     /locus_tag="ACHKG5_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0869271.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9908..10291
                     /gene="nrfF"
                     /locus_tag="ACHKG5_003222"
     CDS             9908..10291
                     /gene="nrfF"
                     /locus_tag="ACHKG5_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0869272.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10288..10884
                     /gene="nrfG"
                     /locus_tag="ACHKG5_003223"
     CDS             10288..10884
                     /gene="nrfG"
                     /locus_tag="ACHKG5_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0869273.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11226..12539
                     /gene="gltP"
                     /locus_tag="ACHKG5_003224"
     CDS             11226..12539
                     /gene="gltP"
                     /locus_tag="ACHKG5_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0869274.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12717..13406)
                     /gene="yjcO"
                     /locus_tag="ACHKG5_003225"
     CDS             complement(12717..13406)
                     /gene="yjcO"
                     /locus_tag="ACHKG5_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0869275.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13500..15647)
                     /gene="fdhF"
                     /locus_tag="ACHKG5_003226"
     CDS             complement(13500..15647)
                     /gene="fdhF"
                     /locus_tag="ACHKG5_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15228..15230),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP0869276.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15845..17311)
                     /gene="mdtP"
                     /locus_tag="ACHKG5_003227"
     CDS             complement(15845..17311)
                     /gene="mdtP"
                     /locus_tag="ACHKG5_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0869277.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLDYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17308..>18684)
                     /gene="mdtO"
                     /locus_tag="ACHKG5_003228"
     CDS             complement(17308..>18684)
                     /gene="mdtO"
                     /locus_tag="ACHKG5_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0869278.1"
                     /translation="FCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAI
                     IEFRQKLASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDP
                     NTPPTPAAKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVI
                     VANPNVGSSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWI
                     ATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVW
                     PESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRV
                     ALERQLDSEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADAL
                     EKYAAGLATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAT
                     EQAQGATQ"
BASE COUNT         4135 a         4958 c         5117 g         4474 t
ORIGIN      
        1 tacgcgcggc cttgctcaac gccaaagccg gtctgggagc ggataaactg cgcgcggaac
       61 agttcacgct cacgcgcgcc ttccgctgag ttatcggttg ccgagaagaa ccagatgccg
      121 aggaatgcca cggtgataga gaacagcgcc gggtattcat acgggaagat ggctttttcg
      181 tgaccgagga tctgtaccca aatcgtcggg ccgaggatca tcagtaccac tgcggtaatc
      241 agccccagcc agccacccat catcgcgcca cgcgtggtca gtttcgacca gtacatagaa
      301 agcagaatga tcgggaagtt acagctcgcc gcgatggcaa acgccagccc caccataaag
      361 gcgatgttct gattctcaaa cagcacgccg aggataatcg caatcacacc caaaatcagg
      421 acggtgattt tcgatacccg cagctcttca cgttcggtcg cgcctttttt gaagacgtta
      481 gcgtacaggt catgcgaaac cgctgatgcg cccgccagcg tcagacccgc aaccaccgcg
      541 aggatagtgg cgaaagcaac cgctgaaata aagccgagga acaggttgcc gcccaccgca
      601 ttggccaggt gaactgctgc catgttgtta ccaccaatca gatggcccgc cgcgtcttta
      661 tattccggat tcgcaccgac cagcatgatc gcgccgaagc cgataataaa ggtcaggata
      721 tagaagtagc ccataaatcc ggtagcgtag aacacgcttt tacgcgcttc gcgggcatcg
      781 ctgaccgtaa agaagcgcat cagaatgtgc ggcaagccag ccgtaccaaa catcagtcct
      841 agccccagag agagtgcgga gatcggatct ttcaccagtc cgcccggttt catgatgtcg
      901 acacctttcg ggtgtaccgc catcgcttcg ctgaacagat tgttgaagct aaagccgacg
      961 tgtttcatca ccataaaggc cataaagctg gcaccgaaca gcaacagcac agctttgata
     1021 atctgtaccc aggtggtcgc cagcatgccg ccgaacagga cgtacatcat catcagcacg
     1081 ccgaccagca ccactgcaat gtgatagtta aggccaaaca gcagctcgat cagtttacct
     1141 gcacccacca tctgggcgat aaggtaaagc gccaccacca ccagagaacc acaggccgaa
     1201 agaatacgaa tcggcccttg tttcagacga taagaggcta catcggcaaa ggtgtaacgc
     1261 cccaggttac gcagacgttc ggcaatcagg aacaaaatga tcggccagcc caccaggaag
     1321 cccagcgagt aaattaagcc gtcatagccg gaggtaaaca ccagtgcgga gatccccaaa
     1381 aatgaggcgg cggacatata gtcaccggca atcgccagcc cgttctgaaa accggtgata
     1441 ttgccgcctg cggtgtaata gtcattacgc gaacgtacgc gttttgacgc ccagtaggta
     1501 atgccgagcg taaacacgac gaaaatcagg aacataataa tcgcctgcca gttcgttggc
     1561 tggcgctcta cggccccgct aatagcatcc gcggcgttag ctgcgaaagg gagtgtggcg
     1621 gcaagcgccg tcagaactct tttcatgatg cttgtacctc atgcaggact tcattattaa
     1681 gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc agtgagaaca aaggagatca
     1741 ctattacccc aacaccgact ggaataccgc gtgtgacgct ggtgttcgga ttcagcgggg
     1801 tgcccagcca gccgggcgcg aaggcgatca gtaaaataaa gccgatataa actgccagca
     1861 taataatcga caggatggtg gcaaaccgtt gccgtttttc gactaactcc ctgaaatgcg
     1921 cattgtcttc tatccgctga taaatagtgc cattcatcac agattctcca gaggtaatgt
     1981 agggattgtt ttaattcccg ctcccttatg ggagaaggtt aacgctcggg taacccttgc
     2041 cgaatgtagg ccggataagg cgttatacgc cgcatccggc aatcaatgcc tgatgcgacg
     2101 ctgtcgcgtc ttatcaggcc tacaaaccgt taccgactcg catccggcaa ttgtgggtta
     2161 cgatggcatc gcgatagcct gcttctcttc aagcagcttc tcgactacgc caggatcggc
     2221 aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc gcagaatacg
     2281 gcgcataatt ttgccggagc gggttttagg cagggagtcg gtccagtgca gcacgtctgg
     2341 cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt acagttctgg
     2401 tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac ctttaatatt
     2461 gtgcggaata ccgacgacag cggcttcggc gattttcgga tgcgccacca gcgccgactc
     2521 aatctccgcc gttcccagac ggtgaccgga gacgttcagc acatcgtcca cacgcccggt
     2581 gatccagtaa tagctatctt catcacgacg cgcgccgtcg ccgctgaaat acatattttt
     2641 gaaggtggaa aaataggtct gctcaaaacg ttcgtgatcg ccaaacagcg tacgcgcctg
     2701 acccggccag gagtcggtga tcaccaggct accttcggta gccccttcca gcgggttacc
     2761 ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg aaccggcttt
     2821 cagctcggta gcgccaggca gcggggtgat catgaaaccg ccggtttcgg tctgccacca
     2881 ggtatcgacc accggacatt tctcgttgcc gatttttttc cagtaccact cccacgcttc
     2941 cgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt cggtgccttc
     3001 gatcgcttta tcgccttccg ccatcagcgc gcgaatcgcc gtgggcgcgg tatagagaat
     3061 attgacctga tgcttgtcca ccacctgtgc catacgggca ggcgtcggcc agttcggtac
     3121 gccttcaaac atcagcgtgg tcgcgccgca ggccagcggg ccgtacagca aataactgtg
     3181 tccggtcacc cagcccacat cggcggtgca ccagtagata tcgcccggat gataatcaaa
     3241 gacatattta aaggtcagcg ccgcgtacac cagataaccg ccggtagtgt gcagtacgcc
     3301 ttttggtttt ccggtagaac cggaggtata gagaataaac agcggatctt cggcgttcat
     3361 ctcttccgcc tggtgctgat cgctggcttg ctcaacctgg tcgtgccacc acaggtcgcg
     3421 cccttcctgc cagtcaattt tcccgccagt acgcttcagt accaccacat gctctacgct
     3481 ggtgacgttc gggtttttta gtgcgtcatc aacgtttttc ttcagcggaa tactacgccc
     3541 ggcgcgcacg ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcga taatgcgccc
     3601 ggcaaccgct tccggcgaga agccaccgaa aattaccgaa tgcaccgcgc caatacgggc
     3661 gcaggccagc atcgcaaccg cggcttccgg caccatcggc atataaatcg ccaccacatc
     3721 acctttttta atgcccagct tgagcagggt attggcgaag cggcagacgt cgcggtgcag
     3781 ctctttatag ctgatatgtt tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt
     3841 acgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat tcagcgtgcc
     3901 gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtgtttt tcaccttctg
     3961 gtacggtttg atccagtcga gaatttttcc ctgttcgccc cagaaggtat caggtgcgtt
     4021 aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac ggtctgcgat
     4081 gttggcagga atggtgtgtt tgtgaatttg actcatgctt ttgttctcct tgtaggatgt
     4141 taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt tagtatttgg
     4201 gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt tttgaaggtc
     4261 agcaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgctg ttgaaaaaga
     4321 ggaagatact gactaactct aaagtggtat tttacatgca cttacaattg attaaagaca
     4381 acattcacag tgtggttatt tgttacactt aggggcgagc aatgtcatga cagtgtaggt
     4441 gcggttactg tcgtgaaaaa caataaaaac cgccattgca acaatggcgc aattcggatg
     4501 aagcccctat gacaaggata aaaataaacg cacgccgtat cttcagctta ttgattcctt
     4561 ttttcttttt cacttctgtt cacgctgaac aaacggctgc tcccgcaaaa cctgtaactg
     4621 tggaagcgaa gaatgaaacc tttgccccgc agcatcccga tcaatatctc tcctggaaag
     4681 ccacctcgga acagtcagag cgtgttgacg cccaggcgga agatccacgg ctggtgatcc
     4741 tgtgggcggg gtatcccttc tcgcgcgatt acaacaagcc gcgtggacat gcctttgctg
     4801 tgaccgatgt gcgtgaaacc ctgcgtaccg gtgcgccgaa aaacgctgaa gatggtccgc
     4861 taccgatggc gtgctggagt tgtaaaagcc cggatgtggc gcgtctgatc cagaaagacg
     4921 gcgaagatgg ctacttccac ggtaaatggg cgcgcggcgg cccggaaatc gtcaacaact
     4981 taggttgtgc cgattgccat aacaccgcct ctccagagtt cgccaaaggc aaaccggagt
     5041 taaccctttc ccgtccgtat gcggctcgcg cgatggaagc cattggtaaa ccttttgaga
     5101 aagccggacg tttcgaccag caatcgatgg tttgcggtca gtgccatgtg gagtattact
     5161 tcgacggcaa aaacaaagcg gttaaattcc cgtgggatga cggcatgaaa gtcgaaaata
     5221 tggagcagta ttacgacaaa attgccttct ctgactggac taactccctg tcgaaaacgc
     5281 caatgctgaa agcgcagcac ccggaatatg aaacctggac agcgggcatt cacggtaaaa
     5341 acaacgtgac ctgtatcgac tgccatatgc caaaagtgca gaacgccgaa ggcaaactct
     5401 acaccgacca taaaattggt aatccgtttg ataacttcgc ccagacttgt gcgaactgcc
     5461 atacccagga caaagctgcc ttgcaaaaag tggtcgcgga acgtaagcag tcgattaacg
     5521 acctgaaaat caaggttgaa gatcaactgg ttcacgctca cttcgaagcg aaagcggcgc
     5581 tggacgcagg tgcgacggaa gccgaaatga agccgattca ggacgatatc cgtcatgccc
     5641 agtggcgttg ggatctggcg atcgcttccc acggcattca tatgcacgca ccggaagaag
     5701 gtctgaggat gctcggtacg gcgatggata aagcggcgga tgcacgcacc aaactggcgc
     5761 gcctgctggc gactaaaggc atcacccatg aaatccagat cccggatatc tcgaccaaag
     5821 agaaagccca gcaggccatt ggcctgaaca tggaacaaat caaggccgag aagcaggact
     5881 tcatcaaaac ggtgatcccg cagtgggaag agcaggcacg taaaaacggt ctgttaagcc
     5941 aataaccccg ttccgcctcg caaggggcgg aaaacacaat ggagtgaata tgagcgtatt
     6001 acgttcgtta ttaactgccg gggtgctggc gtcgggcctg ttgtggagcc tgaacggaat
     6061 taccgctacc cctgcggcgc aggcatctga tgatcgctac gaagttaccc agcagcgtaa
     6121 cccggatgcc gcctgtctgg actgtcataa gccagatacc gaaggtatgc atggcaaaca
     6181 tgcttccgtt atcaacccga ataacaaact gccggtcacc tgcaccaact gtcacggcca
     6241 gccatcaccg caacaccgcg aaggggtgaa agatgtgatg cgctttaacg agccgatgta
     6301 caaggttgga gagcagaaca gcgtctgtat gtcctgccat ctgccggaac agttgcaaaa
     6361 agcgttctgg ccgcacgatg tccacgtaac caaagtggcg tgcgccagct gccattccct
     6421 gcatccacag caagatacga tgcagacgtt aagtgacaaa ggacggatta agatttgcgt
     6481 cgattgccac agcgatcagc gcaccaatcc gaactttaac ccggcgtctg ttccgttgct
     6541 taaggagcag ccatgacctg gtctcgtcgc cagtttctca ccggcgtcgg cgtgctggca
     6601 gccgtcagcg gcaccgcagg gcgcgtggtg gcgaaaacgt tgaatatcaa tggggtgcgt
     6661 tacggcatgg tgcatgacga gtcgttatgc atcggctgta ccgcctgtat ggatgcttgt
     6721 cgggaagtga acaaggtgcc ggaaggcgtc tcgcgcctga cgattattcg tagcgagccg
     6781 cagggcgaat ttcctgatgt gaaatatcgc ttcttccgta agtcttgcca gcactgcgat
     6841 catgcgccgt gcgttgacgt ctgcccgacc ggtgcatctt ttcgcgatgc tgccagtggg
     6901 attgtcgatg ttaatccgga tctctgcgtc ggttgtcagt actgcatcgc cgcctgtccg
     6961 taccgcgtgc gctttattca tccggtcacg aagacagcgg acaaatgcga tttctgccgt
     7021 aagaccaatt tgcaggccgg taagcttccc gcgtgcgttg aggcttgccc gacgaaggcg
     7081 ctgacgtttg gcaatctgga cgatcccaac agtgagattt cgcaactgct gcgccagaag
     7141 cccacttacc gctacaagct ggcgctggga accaaaccga agctgtaccg cgtaccgttt
     7201 aaatacgggg aggtgagcca atgacgcaga cttccgcatt tcattttgaa tcgctggtgt
     7261 gggactggcc gattgccatc tacctgtttt tgattggtat ttctgccggt ctggtgacgc
     7321 tggccgtgct gttacgtcgc ttctacccgc aggcgggcgg tgcagacagc acgttgctgc
     7381 gcaccacgct gattgtcgga cctggcgcgg tgatcctcgg tctgttgagc ctcgtcttcc
     7441 acctgacaag accgtggacc ttctggaagc tgatgttcca ctacagtttt acttcggtga
     7501 tgtcgatggg ggtgatgctg tttcagcttt acatggtggt gctggtgctg tggctggcga
     7561 aaatctttga acatgatttg cttgccctgc aacaacgctg gttgccgaag ctggggatcg
     7621 tgcaaaaggt tctgagcctg ctgacgcccg ttcatcgcgg actggaaaca ttgatgctgg
     7681 tgctggcggt gttgttgggg gcttataccg gttttctgct gtcggcgctg aaatcgtatc
     7741 cgttccttaa taacccgatc ctgccggtgc tgttcctctt ctccggcatc tcgtccggtg
     7801 cggcggtggc actgatcgcc atggcgatac gccaacgcag taacccgcat tccacggaag
     7861 cgcagtttgt acaccgtatg gaaatccccg tggtatgggg tgaaatcttc ctgctggcgg
     7921 cgttttttgt cggtctggcg ctgggcgatg acggtaaagt gcgtgcgctg gtggcggcat
     7981 tgggcggcgg tttctggacg tggtggttct ggcttggtgt cgccgggctg gggctgattg
     8041 tgccaatgtt gttcaaaccg tgggtcaatc gcagttccgg cattcctgcc gtgctggcgg
     8101 cgtgtggggc cagtctggtc ggcgtgttga tgttgcgatt tttcattctc tacgccgggc
     8161 agttaacggt ggcgtaagcc agaacagagg tggtttctgg acgtattcct tcctgaagtc
     8221 ggttttctgg cgctgttgtt aagtctcggg gtcaacgtgt tgaccccgtt gacggccttt
     8281 gcgggagtgc ggttgcgctg gcctgccatg atgcgactca cttgcatcgg cattctggca
     8341 cagttcgcgc tcctgctgct cgcctttggc gtactgacgt attgttttct catcagcgat
     8401 ttctcggtca tttatgtcgc ccaacatagc tacagcttgc tgtcgtggaa actcaaactg
     8461 gcggcggtgt ggggcggtca tgaaggttcg ctgctgcttt gggtgctgct gctttccgcc
     8521 tggagcgcgc tgtttgcctg gcaatatcgg cagcaaaccg atccgctatt tccgctgacg
     8581 ctagccgttt tatctctcat gctcgccgca ctgctactgt ttgtagtgct gtggtccgat
     8641 cccttcgtgc ggatatttcc accagcaatc gaaggccgcg atctcaatcc gatgctgcaa
     8701 catcccggtc ttatctttca tccaccgctg ctttaccttg gctatggcgg tttgatggta
     8761 gcggcgagcg tggcgctggc gagtctactg cgcggcgagt ttgatggtgc ctgcgcccga
     8821 atttgctggc gctgggcgtt acctggctgg agtgcattaa ctgcggggat catcctcggt
     8881 tcctggtggg cctactgcga actcggctgg ggcggctggt ggttctggga tccggtggaa
     8941 aacgcctctt tattaccctg gctttctgcc actgcgctgc tgcacagttt atccctgaca
     9001 cgccagcggg ggatttttcg ccactggtcg ctgttgttgg cgatagttac tctgatgctg
     9061 tcgctgctgg gcaccttaat tgtccgttct ggcattctgg tttcggttca tgcgttcgct
     9121 ctggataacg ttcgcgctgt gccgttgttc agcctgtttg cactgattag ccttgcgtct
     9181 ctggctctgt atggctggcg agcgcgggac ggtggcccgg cggtgcgttt ttcggggtta
     9241 tcgcgggaaa tgttaatcct cgctacgctg ttgctgtttt gcgcagtgct actgatcgtg
     9301 ctggtgggaa cgctttatcc gatgatttac ggcctgctgg gctggggacg tctctccgtt
     9361 ggcgcaccgt attttaaccg cgcgacgtta ccgtttggtc tgttgatgtt ggtggtgatt
     9421 gtgctggcga cgtttgtctc tggcaaacgc gcgcagcttc cggcgctgct ggcgcatgcg
     9481 ggcatactgt tatttgccgc agggatcgtt gtctccagcg tcagccgtca ggagatcagc
     9541 ctgaatttac agccgggtca gcaggtgacg ctggcaggat acaccttccg ttttgagcgc
     9601 ctcgatctgc aagccaaagg caattacacc agcgaaaaag cgatagtggc gctgtttgac
     9661 catcagcaac gcattggtga attaacgccg gagcggcgtt tttacgaagc tcgtcgtcag
     9721 caaatgatgg aaccgtcaat tcgctggaac ggcatccatg actggtatgc ggtcatgggg
     9781 gagaaaactg gagcggatcg ttacgctttt cgcttgtatg tacaaagcgg tgtgcgctgg
     9841 atctgggggg gaggattgtt gatgattgcg ggcgcattgt taagcggatg gcgggggaag
     9901 aagcgcgatg tataaagggc ttctcacgct actgctgtta tttacctgtt ttgcgcgcgc
     9961 tcaggttgta gacacctggc aattcgccaa tccgcaacaa cagcaacagg cgttaaatat
    10021 tgccagccag ttacgttgtc cgcagtgcca gaatcaaaac ttactggaat ccaacgcgcc
    10081 ggtggcagtc agtatgcgcc atcaggttta cagcatggtg gcggagggga aaagtgaagt
    10141 cgaaatcatt ggctggatga ccgaacgcta cggagatttt gttcgctata acccaccgtt
    10201 aacgggtcag acgctggtgt tgtgggcgct gccagtggtg ttgttactgc tgatggcact
    10261 gatcctctgg cgagtgaggg cgaagcgatg aaacagcctc aaataccggt gaaaatgctg
    10321 acaacactca cgatattgat ggtattcctc tgtatcggta gttatctgtt aagcccaaaa
    10381 tggcaggcgg tacgtgcgga gtatcagcgt cagcgcgatc cgctacatca gtttgccagc
    10441 cagcaaaacc cggaagcgca gcttcaggca ttgcaggata aaatccgcgc taatccacaa
    10501 aacagcgaac agtgggcgtt actgggcgag tactatctgt ggcaaaacga ttacagcaat
    10561 tcgctgctgg cgtaccgtca ggcgttgcaa ctacgtggtg agaacgctga actgtatgcg
    10621 gcgctggcga cggtgcttta ttaccaggcc agccagcata tgaccgccca gactcgcgca
    10681 atgatcgaca aagccctcgc gctggactct aacgaaatca ccgctctgat gctgctggct
    10741 tccgatgcgt ttatgcaggc gaattacgcg caagccatcg aactatggca aaaagtgatg
    10801 gatctcaact caccccggat caaccggaca cagctggtcg agtcgattaa tatggcgaaa
    10861 ttgttgcagc ggagatcgga ttaatcatct ggtgatgaag attatgttat tgagctgcat
    10921 aaaaaataat cgaatgaaca tatgccaaaa ataatcacta atcagtatta ttgcagatta
    10981 aacaaataaa aatctttcca taacaaatgg ttattcatta atcctgccat aaactcgcgt
    11041 tattatgcat taatgcagcg aaaagctctg ttgttaaagg gttgcgcaac ataccgcgca
    11101 aatgatactg atcataagcg ttaaaaaaat ctacaaacca acgcaacaca attcatgccc
    11161 tggcagtatg ccacgttctc gcgtttctga acggggaacg gcgctccatt gaggaagtca
    11221 ttcatatgaa aaatataaaa ttcagcctgg cctggcagat tctgtttgct atggtgctgg
    11281 gcattctcct gggaagctac ctgcactacc atagcgacag ccgcgactgg ctggtcgtca
    11341 atttgctctc tccggcgggt gatatcttca tccatctgat caaaatgatt gttgtgccga
    11401 ttgtgatctc cacgctggtg gtgggtatcg cgggtgttgg tgatgccaaa cagctcgggc
    11461 gtattggcgc gaaaaccatt atctacttcg aggtgatcac caccgttgcc atcattttgg
    11521 ggatcactct ggcgaacgtc ttccagcccg gtgccggggt ggatatgtcg cagctggcga
    11581 ccgtcgatat ctcgaaatat cagagcacta cggaagcggt acaaagcagt tcccacggca
    11641 ttatgggcac gattttgtcg ctggtgccga cgaatattgt ggcgtcgatg gcgaaaggcg
    11701 aaatgttgcc gatcatcttc ttctcggtgc tgtttggtct ggggctttcc tccctgcccg
    11761 cgacgcatcg tgaaccgctg gtgaccgtgt tccgctccat ctctgaaacc atgtttaaag
    11821 tgactcacat ggtgatgcgt tatgcgccgg tgggtgtgtt tgcgctgatt gcagtgacgg
    11881 tggctaactt tggtttctct tctctgtggc cactggcgaa actggtgctg ctggtgcatt
    11941 tcgccattct gttcttcgcg ctggtagtgc tgggaattgt ggcgcgccta tgcgggttaa
    12001 gcgtctggat cctgattcgt attctgaaag atgagctgat tctggcgtac tccactgcca
    12061 gctctgaaag cgtactgccg cgaattattg agaagatgga agcctacgga gcaccggcgt
    12121 cgatcaccag cttcgtggtg ccgaccggtt actcttttaa ccttgatggt tcgacgctgt
    12181 atcaaagtat tgccgctatt ttcatcgctc agctgtatgg cattgacctg tccatctggc
    12241 aggaaatcat tctggtgctg acgctgatgg tgacctcgaa agggattgct ggcgtgccgg
    12301 gcgtgtcgtt tgtggtgttg ctggcaacgc tgggtagcgt aggtattccg ctggaaggtc
    12361 tggcgtttat tgctggtgtt gaccgtatcc tcgacatggc gcgtactgca ctgaacgtgg
    12421 tgggtaatgc gctggcggtg ctggtgattg ccaagtggga acacaaattt gaccgcaaaa
    12481 aggcgctggc ttatgagcgt gaagtgctgg gcaaatttga taaaactgcg gatcaataat
    12541 tgaagattgc cggggatatc ccccggcaat gtgtggatgc ctgatgcgac gctggtgcgt
    12601 cttatcaggc ctacgtcaga cagcgctagc gtgattttgt aggtctgata aggcgtttac
    12661 gctgcatccg acatcaacgc ctgatgcgac gcttaacgcg tcttatcagg cctaccttaa
    12721 ccgttagtta atttttcaaa ctcttcacac ccggtatcaa acccttccat acagctcagg
    12781 ttcaaccagt gcagcgcctt ttgcttgttc ttctcgataa aaccttcttc accgtttaag
    12841 aacatcattc ccgcccagta ctcggaataa ccggttcggg aaattgcaga gctgcgtttg
    12901 aaataccagg ttgccttgtc atcgtcggcc ttaatgccca cgccgttggc gtaaatcaaa
    12961 ccaagcagca tttgggcatc gacggcagag tcgttctcca gatcttccga ggcgttttct
    13021 aacagcgaaa tcgcctttgg ataatccggt ttacccgctt gagtatttac cagaatatgc
    13081 gccagcgtta cttcaccttc tttactgccc gcttgcgccg ctttctctgc aagaactttt
    13141 gcttgtggat agtccagact gaccggattg gtgattttga tctgcgccaa cagcgcgcag
    13201 gcatcagcat caccgctgtc ggcggctttc tgtgcccaat attcggcttt gctcaaatcg
    13261 ccggagctaa accagctgtc ggcaagaaaa tattgtgcgc gtcggtcccc ggcctctgct
    13321 gcctttaaat actggctgcc aggctcggag tcgttggcgt gggcaaagaa tgtcaaaaac
    13381 aacatcaatg cgataatttt tttcattttg ataatacgat tgggtaggga gtaaccagta
    13441 taaagagttc gaatggataa aaaaacagcc tccgaaagga ggctgtagaa aggactgtat
    13501 tacgccagtg ccgcttcgcg caggcgagtt ttcaacttgt tgtactcgtc aatcacgtac
    13561 tgctcggcgg cgcgctgatc ggcgatcggc tcgacgcgca cggcgcagta tttgtattcc
    13621 ggcgttttcg taatcgggct taagttttcg gtaaccagct cgttacaggc accaatccac
    13681 cactggtagg tcatgtaaat cgcccctttg ttcggacgat cgctgacctg cgcacgggtg
    13741 ataattttgc ctttacgcga gtgcacccaa accaatgcct catcttcaat actcagacgt
    13801 ttggcgtctt cggtattgat ttgtgcgtag ccaggttcat cagccagcgc cgccagtgcc
    13861 gcacagttac cggtcatcga acggcaagag tagtgaccaa cttcacgcac cgttgacagt
    13921 accatcgggt actcgtcggt gagtttgtcg attggcgcta cccagtcgca ggtgaagaac
    13981 tgcgccagac cgttcggggt gtcaaacttc tctttaaaca gataagaagt cccctgatcg
    14041 gcatctgaag tatcgcggca aggccactga atgaagccca gttcgcccat tttctcgtaa
    14101 gtcgcaccgt agaaatccgg gcacagatga cgcaactcat cccagatctc ctgggtgttg
    14161 ttgtagtgca tcggataacc catacgggtg gcgatttcac tgatgatttg ccagtccgtt
    14221 ttcagatccc atttcggttc aactgctttg aagaaacgct ggaagccacg gtcagccgca
    14281 gtaaacacgc cttcatgctc gccccacgac gttgacggta aaataacatc cgccgccgac
    14341 gcggttttgg tcataaagat gtcctgaacg ataaccagtt ccagatcttc aaaggcttta
    14401 cgtactgccg acagctccgc gtcagtttgt agcggatctt cgcccataat gtacgcggca
    14461 cgcacttcgc catgcgctgc gcggtgcggc agctcgctga tgcgataacc ggtatgagca
    14521 ggcaggcttt ccacgcccca ggctttggcg aatttctcgc ggttagccgg atcttttacg
    14581 tactggtatc ccggatacgt atccggcagc gcgcccatat cgcaggcacc ctgcacgttg
    14641 ttctgaccac gaaccgggtt aacacccgca tgtggcttac cgaggttacc ggtcagcatc
    14701 gcgaggctgg tcagagaacg cacggtttcc acgccctggt agaactgggt tacacccatg
    14761 ccccacagga tggcggcgct tttcgcctgg gcatacatcc gtgccgcctg acgaatctca
    14821 ctggcgctga cgccggtgat atcttcaacc gactccggcg tgtagccttc aacgatttta
    14881 cgatactctt caaagccttc tgtacgggaa gcgacgaatg ctttgtcgta cagattttct
    14941 tcaataatga catggcccat cgcattcaac agcgcgatgt tcgagccgtt tttcagtgca
    15001 atgtgcatgt cagcaatgcg cgcggtttca attttgcgcg gatcgcagac gataattttc
    15061 gccccgttac gtttagcgtt aattacgtga ttcgccacga ttgggtggga atccgccggg
    15121 ttgtacccga aaacgaacac taaatcggta ttatcaattt cgttaatagc attgctcatt
    15181 gcgccattac cgaccgattg gtgcagacct gcaaccgatg ggccgtgtca gacacgagcg
    15241 cagcagtcaa cgttattggt accaataacg gcgcgcgcaa atttttgcat tacatagttg
    15301 gtttcgttac ccgtaccacg cgaggagccg gtcgtctgga tggcatccgg accgtacttc
    15361 tctttgatgg cgctcaggcg ctcggcaacg taattcagtg cctcatccca ggaaacaggt
    15421 tcgagtttgc cgccacgctg gcgacggatc atgggggttt tcaggcgcgg ggtcaggatc
    15481 tgggtatcgt taatgaagtc ccagccataa taacccttca gacacagggt accctggttg
    15541 gttttcccct gcgctgcctc cgcccggacg attttgccgt tatcgacgac caggttgatt
    15601 ttgcaacctg atgcgcaata ggggcaaacc gtgacgactt ttttcatcgg tctcgctcca
    15661 gttaatcaaa tcacgcatac gcgctctcga ctacagtatg catcttttat gccacatttt
    15721 atttggggtc attccctgat attacgggca ctatttattc aaaactctga cgaaaaacag
    15781 gctgtcgtca gttttgacgt gacgaaacga aataccgcgt gacagccatc acacggcaga
    15841 cattttattt tttctcgacg acgggagccg cctgataccc gctgcccagc gatttcatca
    15901 actgaatgct ttggatcacc cggcggctgt ccagcatcag taatgacatc tcttcggcaa
    15961 gcaccggcaa ccgggcttcg gtagcctgta agcggctggt taagccgcgc tgataggcgg
    16021 cctcggcagc gcgctgggtg aagcgcgttg cttccacgcg ttcagcctgc atttctcgct
    16081 cgtcgttgag cgtttgcaga cgcgtgccgt tgacggcaac gtcacgcacc gcgttcagta
    16141 ctgactggtt gtaacgttca atcatcatgt tgctggcggc gcgagtgcct tcgagattgg
    16201 cattcaaccg tccaccgtca aacagcggca atttcagacc cgggatgaag ttgaactggc
    16261 gactggtttt tttgaataag gtatccaggt ggatggagtc cagaccgaaa aacgctttga
    16321 tatcaaagct tgggtagaac agcgcccgcg cggaatccac ctgatctaat gacgcctgaa
    16381 cataccagcg catggcttgc agatccgggc gtctggcgag caactcataa gagagtgtcg
    16441 ccggaatacc ggtctggact cgtggtaatg ccactggttt gatctccggc atatcgctgg
    16501 ctcccgcgcc aatcaatgca cgcagagatt ctcgcgtttc ggtgatttgc cctttaacgg
    16561 cagcaatttg tttatcgacc gccagaatct gtgcccgcgc gccgtggaaa ggcacttgcg
    16621 cttccagacc gtgcgccact ttactttggt gcgctttcac cgcgtaatca atcacatcgc
    16681 gagtttgttc taacagatcg agcatctgat agctggcctg catactgtaa tcaagctgcg
    16741 ctacgcccgt ggtcagcgat agctctactg ctgcggtttc tgccagcgcg gcattatgcg
    16801 cgccaatggc ggcggtaacc gctgagcgat gcacacccca caaatcaaga tccagtccgg
    16861 caaacaaacc gactgtggct tccgtatagt acggcccgtc catacccagt gctggcgcat
    16921 ccatcgcata agggcttaaa aagccgttcg ccgagacgcg ttggcggttg agcatcccta
    16981 acgctgcgac ctgtaattgt gaaccggcat ctaacaaatc ggcctgggac tgcgcttttt
    17041 cttcccgcag tttcgcttcg gcgagggtgt gtgaaccact tagcgtccgt tggatcagcg
    17101 catccagctg cgggtcattg agttgtttcc accactgcgc ctgcggccag ccggagctgg
    17161 caagatgaat atcatcggcc agtttgattt gttccggttt gagctgttga tgaggcgcag
    17221 aatccttgcg taccagggca cagccggaaa tcagcgtcgt gctgccgaga atgctgcaca
    17281 acagcagacg tgaaagttga cgattgatca ttgcgtggct ccttgtgcct gttccgtggc
    17341 gggcgttaat gccggggctg tccagtctgg caagcgggcg aaaagctggc agacgtgttg
    17401 ctcctgtttt aataaggtgg gcaggatggc ctgcgaggcg ggtgtctctt caagcgttat
    17461 ttgaggagaa cggctgagtg cggttgccag accggcagcg tatttttcca gggcgtcggc
    17521 aaactgacct gctttgtccg gctgtagtgc gttatccagc gcctgcgccg agttccaggt
    17581 ggcatcccag gcgtgaagaa gatcgcggcc ctgatgaata accgtttgcg aacgttcaat
    17641 cagtaaggcg cgttcttcgc tgtccagttg acgctccagc gccacgcgtt ggcacatctc
    17701 ttcacaggca ttaaacgccg catgcagacc tatacgaatt tgcagataag tgcgcagagc
    17761 cgtgacttcc tgctggcgtg ggatccgcat tactttactt agcataccca gcgcgccagc
    17821 cagtttttgc ggcagcgtgc gcgcttcact ttcaggccag acaaaggtgt aaatcaccgc
    17881 agacaccacg gtaccaatga tgatacccag ggcgcgatcg cgaatttcca ccaggtcgta
    17941 caccggacca aaaacgtttt cgagcgtggc gagcgcgaag gtgaccacca tctgtgtgcc
    18001 gatataagaa gagcgttcag agctggtggc aatccatgcg cccaacagga aaatcggtgc
    18061 cagcacaaac agcaattcga caatattgtc cagccagggc atgaccagta gcgtgaataa
    18121 cagcgccaga atcgcgccgc aaaaggcccc gccaaaacgc agcaccatct tctggtacga
    18181 tgaaccgaca ttcggattag cgacgatcac gcaggtcagc atacaggtgt gaatgccttc
    18241 ccagtccacg ccgctgtaga aggtgtaaca gatcaaacag gcgagcagcg tttttaccgc
    18301 gtagcgcata tagtctggat tggtaaaagc atcagcgacc attgatggcg gtttggcggc
    18361 gggcgttggc ggcgtattcg ggtccatctg acccagttgt aacaacgtct ggcagatatt
    18421 ctccaggtta cattcccgtg ccgccatcgc ttcactttca ctgatccgcc agtcgctttg
    18481 ccagcactga ccttcggtaa tggcatgctg cagcttgttg atttctgaag ctaatttttg
    18541 tcggaattca ataattgcct gagaatcagc aaaagaggtg ggatcgtagc gattcagcgt
    18601 cgagtaaatg taggttaccg ttgccacgca gctttgccac catgcgcttt gagttcgcca
    18661 gttggcatcg tccgcgaggc aaaa
//
LOCUS       DAZGXS010000090        18435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGXS010000090 DAZGXS010000000
VERSION     DAZGXS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            183..1403
                     /locus_tag="ACHKG5_003229"
     CDS             183..1403
                     /locus_tag="ACHKG5_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0869279.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1376..2005
                     /locus_tag="ACHKG5_003230"
     CDS             1376..2005
                     /locus_tag="ACHKG5_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0869280.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2006..3166)
                     /gene="ybdL"
                     /locus_tag="ACHKG5_003231"
     CDS             complement(2006..3166)
                     /gene="ybdL"
                     /locus_tag="ACHKG5_003231"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0869281.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3275..4363
                     /gene="hcxA"
                     /locus_tag="ACHKG5_003232"
     CDS             3275..4363
                     /gene="hcxA"
                     /locus_tag="ACHKG5_003232"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0869282.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4373..4570)
                     /locus_tag="ACHKG5_003233"
     CDS             complement(4373..4570)
                     /locus_tag="ACHKG5_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0869283.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4752..6857)
                     /gene="cstA"
                     /locus_tag="ACHKG5_003234"
     CDS             complement(4752..6857)
                     /gene="cstA"
                     /locus_tag="ACHKG5_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0869284.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7038..7451)
                     /gene="entH"
                     /locus_tag="ACHKG5_003235"
     CDS             complement(7038..7451)
                     /gene="entH"
                     /locus_tag="ACHKG5_003235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0869285.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7454..8200)
                     /gene="entA"
                     /locus_tag="ACHKG5_003236"
     CDS             complement(7454..8200)
                     /gene="entA"
                     /locus_tag="ACHKG5_003236"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0869286.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8200..9057)
                     /gene="entB"
                     /locus_tag="ACHKG5_003237"
     CDS             complement(8200..9057)
                     /gene="entB"
                     /locus_tag="ACHKG5_003237"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0869287.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9071..10681)
                     /gene="entE"
                     /locus_tag="ACHKG5_003238"
     CDS             complement(9071..10681)
                     /gene="entE"
                     /locus_tag="ACHKG5_003238"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0869288.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10691..11866)
                     /gene="entC"
                     /locus_tag="ACHKG5_003239"
     CDS             complement(10691..11866)
                     /gene="entC"
                     /locus_tag="ACHKG5_003239"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0869289.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12055..13011
                     /gene="fepB"
                     /locus_tag="ACHKG5_003240"
     CDS             12055..13011
                     /gene="fepB"
                     /locus_tag="ACHKG5_003240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0869290.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13015..14265)
                     /gene="entS"
                     /locus_tag="ACHKG5_003241"
     CDS             complement(13015..14265)
                     /gene="entS"
                     /locus_tag="ACHKG5_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0869291.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14376..15380
                     /gene="fepD"
                     /locus_tag="ACHKG5_003242"
     CDS             14376..15380
                     /gene="fepD"
                     /locus_tag="ACHKG5_003242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0869292.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15377..16369
                     /gene="fepG"
                     /locus_tag="ACHKG5_003243"
     CDS             15377..16369
                     /gene="fepG"
                     /locus_tag="ACHKG5_003243"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0869293.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16366..17181
                     /gene="fepC"
                     /locus_tag="ACHKG5_003244"
     CDS             16366..17181
                     /gene="fepC"
                     /locus_tag="ACHKG5_003244"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0869294.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17178..18311)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKG5_003245"
     CDS             complement(17178..18311)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKG5_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0869295.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4355 a         5216 c         4731 g         4133 t
ORIGIN      
        1 tcacgattta ttgaccgtct aaatgagtgt ttttgatata taacctcaga cagctactat
       61 aactttcacc tatttattca cagcgcgaat gaaaaataaa acactctgct tttcagtgtt
      121 tactttataa ttaacagtct tatctcactg atcaccctgt aacgttcaga gagcgtcttc
      181 agatgtctat ttataaaatt cctcttccgc tcaatattct ggaagctgca cgagagcgta
      241 ttacctggac actcaacacc ctaccccgcg tttgtgtctc attttctggc ggcaaagact
      301 ctggtttaat gctgcatctg acagccgaac tcgcccgaca aatgggcaaa aaaatctgcg
      361 ttttgtttat cgactgggag gcgcaattct cttgcaccat taactatgtt cagtccctgc
      421 gcgagttgta caccgatgtc atcgaagagt tttactgggt tgcgctcccg cttacgacgc
      481 aaaattccct ttcacaatac caacccgaat ggcagtgctg ggaacctgat gtcgaatggg
      541 tacgtcagcc cccgcaagat gcgataaccg atcctgactt tttctgcttt taccagccag
      601 gcatgacctt cgaacaattt gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg
      661 cggcgatgat gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca
      721 gtttaaataa acaacgtttt gccgacgata aaccctggac tacggccgca ccaggcggtc
      781 atagctggta catttacccc atttacgact ggaaagtggc tgatatctgg acctggtatg
      841 caaatcatca aagtctctgc aacccactgt ataacctaat gtatcaggca ggcgttcctc
      901 tgcgtcatat gcgaatttgc gaaccttttg gcccggagca acgacaagga ttatggctct
      961 atcacgttat cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa
     1021 gtggcggcat ttacgccgga catgataatc atttctatgg gcatcggaaa atcctcaagc
     1081 cagaacattt agactggcaa gaatatgcat tattgctgct caatagcatg ccggaaaaaa
     1141 cagctgagca ttaccgcaat aaaatcgcca tttatttgca ctggtatcag aaaaaaggca
     1201 tcgaggttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc tcctggcggc
     1261 ggatatgcaa agttttactc aataacgatt actggtgtcg ggcattatca tttagtccta
     1321 cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaaaacgt caggaatggg
     1381 ggatactatg caacaacgat taacgcagga tttaacgcaa tttctcgcca gcttgccgga
     1441 agatgatcgc atcaacgcga ttaatgaaat ccgcatggcg atccatcagg ttagcccctt
     1501 tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac agccaactta tgcctaatga
     1561 ttacaaccca aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat ccattgaaat
     1621 agatggtttt acccaaccca ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt
     1681 ggatggtttt caccgccatg aaattggtaa aggctcatca tcattaaaat tacgcttaaa
     1741 aggttatttg ccggtgacct gtctggaagg tacgcgtaat caacgcatcg ccgcgactat
     1801 tcgtcataat cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga
     1861 actcagtcag ttgggatggg acgataataa aattggcaaa gagctgggaa tggacagcga
     1921 cgaagtgctg cgcctgaagc aaattaacgg tctgcaagag ctgtttgccg accgtcaata
     1981 ttcccgcgcc tggactgtca aataactaca gctggcgcag tcgttcagct gctgccagca
     2041 acgtcgattc cttcttggca aaacagagac gaatcagttt atgtgggaag ggatcggcgc
     2101 aaaacaccga tagcggaatc gccgccacgc cgtgctcccg cgtcagccac tggcaaaact
     2161 caacatcatc cagggtagaa accgcgctgt aatccaccag caaaaagtat gtgccttcgc
     2221 acggtaaaat ctccagccga ctttcattta aggcatttac cagaatatcg cgcttctggc
     2281 gataaaagtc cggtaacgca agataatgct caggttctgc acgtagcata tccgcaattg
     2341 ccagctgtgc cggggtattc accgaaaagg tcagatactg atgtaccttg cgaatttcgg
     2401 cgctgatggg cgctggcgca acacaataac ccactttcca gccggtcata tgataggtct
     2461 tgccaaacga tgacaccgcc accgcccgct cacgcagctg cggatgcgcc agcacactgg
     2521 catggccctg ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg acaaaaatct
     2581 cgtgcccggc gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag acagttgcac
     2641 tgggattatg cggagtgttg aggatcacca gtctggtgcg ctcgcttaac aatgcggcaa
     2701 attcctgcca gtcaacgcga aaatgcggtg gttgcagtgc catacgcttc actattcccc
     2761 cagaaagcgc gatggcgggg gcgtaactgt catagctggg atcaaaacaa atcacttcat
     2821 cgccattgcg caccagtgcg gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta
     2881 cggtgatatc gctatcggca tctggttgat agccatacaa acgttccgtt ttctgagcaa
     2941 tcgcctcgcg caaggcctgc acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa
     3001 cgtggtacgc cagccgctcc tgtaaatagc gcggaccatc aaaatcagga aagccttgcg
     3061 acaggttaat cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc
     3121 caagttgtgg aagtttgctt tgtggaatca gagggttatt tgtcattttt attgtacctg
     3181 acgaagttgc gtgttgaagg cactataaca cgatgctagt atttggcaat caagacgttt
     3241 agatgtctaa ataaaacaat aaggacaaca caacatgcct cacaatccta tccgcgtggt
     3301 cgtcggcccg gctaactact tttcacatcc aggaagtttc aatcacctgc acgatttttt
     3361 cactgatgaa caactttctc gcgcggtgtg gatctacggc gtacgcgcca ttgctgcggc
     3421 gcaaaccaaa cttccgccag cgtttggact tccaggggca aagcatattt tgtttcgtgg
     3481 tcattgcagc gaaagcgatg tacagcaact ggcggctgag tccggtgacg atcgcagcgt
     3541 ggtgattggc gtcggcggcg gcgcactact cgacaccgcg aaagccctcg cccgccgtct
     3601 cggtctgcca tttgttgccg ctccaacgat cgccgccacc tgcgccgcct ggacaccgct
     3661 ctccgtctgg tataacgatg ccggacaggc gctgcattat gagattttcg acgacgccaa
     3721 ttttatggtg ctggtggaac cggagattat cctcaatgcg ccgcaacaat atctgctggc
     3781 ggggatcggt gatacgctgg cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga
     3841 aacgttgccg ctaaccgtgc gactggggat caataatgct caggccattc gcgacgtctt
     3901 gttaaacagt agcgaacagg cgcttgccga tcagcaaaat cagcagttaa cgcaatcatt
     3961 ttgcgatgtg gtggatgcca ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg
     4021 ttttacgcgt gtagcggcgg cccatgccgt acataacggt ctgactgtcc tgccacaaac
     4081 cgagaagttt ctccacggca ccaaagtcgc ctacggaatt ctggtgcaaa gcgccttgct
     4141 gggtcaggat gatgtgctgg cgcaattaac cggggcgtat cagcgttttc atctgccgac
     4201 tacgctggcg gagctggaag tggatatcaa taatcaggcg gagatcgata aagtgattgc
     4261 ccacaccttg cgcccggtgg agtccattca ttacctgcca gtcacgctga caccagatac
     4321 gttgcgtgca gcgttcgaaa aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc
     4381 agcgcgcgcc gccttttcca ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt
     4441 aggtcatcgg cgtttgatcg ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat
     4501 caggcatacc aatcatcagc ttcgccgcct gacctaaata ttttccggct tttgccagtg
     4561 aatcaaacat cttggcctcc gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa
     4621 cgccttatcc ggcctacaaa tgctatcaac gcgctcggtc agccccctcg ccccatcggg
     4681 gagagggtta gggtgagggg gaacccacgg cgccatgcca acattcgcca acatcccccc
     4741 tcactctgac tttagtgcgc gccttttgcc tgcaccacga tctcctcgac attttccggc
     4801 attggctcat acggcgtttc tttcgccgtt ggcttcggct ctttcaatgc cgcaagtgcc
     4861 gtcttaatcg agaacagtgc cagaaccacc acgaccacca taaagaagat ggttaacccg
     4921 gcatccagac ggttgttgaa caccagttgc gccagctgtg actcagtata ctgcgatgga
     4981 atattaccgc tgtcgatcat tgcctggaac ttattagcaa tggccaggaa gccgactttc
     5041 gcatccgggc taaacgcttt ctgccagcct gcggtcaggg tacaaatcag cagccaggcc
     5101 gttggtacca gcgccaccca ggcgtaacgt tgacgtttca tcttgaacaa caccacggca
     5161 cagagcatca gcgccatccc tgccagcatc tggttggcaa taccaaacag cggccacaga
     5221 gtgttaatac cgcccagcgg atcgaccaca ccctgatgca ggaagtagcc ccacgccagc
     5281 acgcacagcg ccgttgccag caggttagca ggcagtgaat cggtccgttt caggcccgga
     5341 gaaaccacgc ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc tgcatccacc
     5401 gccgtcagaa taaacagagc ttcaaacaga atggcgaagt gataccagaa cgccacatcc
     5461 atcatgccgc ccagcgcgcc atgcagaatg taggccatcc ccaccgccaa cgtcggcgca
     5521 ccgcccgcac gggaaatgat cgactgttct cccacttcgc tggcaatctg gttgagcgta
     5581 tccggagtaa tcgcaaagcc ccagctactc accacttgcg cggcagaagc gaccacatcc
     5641 gccgtccctg ccggagccag caccgccatc gggctgttca tggcgaagta cacgcccgga
     5701 tcgatgatac aggcggaaac cagtgccata atcgccacga aggattccat taacattcca
     5761 ccgtagccga taaaacaagc ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa
     5821 gagatcagcg catggaagcc agacaccgcg ccacaggcga tggtgataaa caggaacggg
     5881 aacaggttac cggtccatac cgggccagtg ccgtcaacaa atttggtcaa cgcaggcatg
     5941 gtcagcgtcg ggcgcataat caaaatgcct accgccagac caacgatcgt cccgattttc
     6001 aggaaggtag agaggtagtc acgcggagcc agcagcaacc ataccggcag caccgccgcc
     6061 acaaaaccgt aacccaccag catccaggtc agctgcacgc cggtaaagtc aaagtacggt
     6121 gcccaggtcg gactttctgc cacccagccg ccagaaataa tggcgaaaat caggaatacc
     6181 agaccgatga ccgacacttc accaatacgc cccggacgca gatagcgcag gtagatcccc
     6241 atgaacagcg ccagcggaat ggtgaacgca acggtatacg ttccccacgg gctatgagtc
     6301 agggctttca ccacgatcat cgccagtact gcaaggataa tgaccatgat cataaagcag
     6361 gccaccagcg cgatcacccc ggcggttggc cccatctctt ctttgaccag ctcacccagc
     6421 gaacgaccgt cacggcgcgt agaaacaaac agcaccatga aatcctgcac cgcaccggcg
     6481 agaaccaccc cggcgagtag ccagatcatc cctggcaggt aacccatttg cgccgccagt
     6541 accggcccaa ccaacggacc tgctccggca atggccgcaa aatggtgacc gaacagcact
     6601 ttcttgtccg tcggcacata gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc
     6661 gtcgggtcaa ccgccagcac atttttggcg atatacagac cataaaaacg gtaagcgatc
     6721 agatagatac agaccgacgc caccacaatc cacagcgcgt tgatctgttc cccacgattt
     6781 aaagcaatat atcccagagc aaatgctccc attacagaga gcactgtcca gacgaggtat
     6841 ttccctgatt tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt tacattttgt
     6901 ttctataaca tctttgtacg atttaacaac acagcaacca tgtaaatcgg agttgaaacc
     6961 aatatttaac ttaacaatgt taactcgatc actccgttaa ccgagttttc ctgcaatctc
     7021 atataattct gtcgggttca tcccaaaact gccgtaccca gccgacaagt gcagcaacgc
     7081 cgcccctgtt catcgaaaac gacgatttcc cagctctgat tttgccgccc aagatgcagc
     7141 ggctggcaga cgccgcgtac cttgccttca gacaccgggc gatgatgtgt ggcgttaagt
     7201 tccgtgccca ccacacactg cccgtcgcgg gtcatcataa atccggccat cgatcccagc
     7261 gtttcagcca gcgccgccga cgcgccgcca tgcagcaggc caaacggctg atgagtacgg
     7321 atatcaaccg gcatttcggc ttccagcaca tcatcgccca gacgggtata cacaattccc
     7381 agatgcgcca ccattgtgtt atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt
     7441 ttccagatca tgcttatgcc cccagcgttg agccgccatc gaccacaata tcctgtaggg
     7501 tgatatggct ggcgaggtca gaggcgagga acaaaatcgt gttggcgatc tcttgtggac
     7561 gggcgatttt ccccagcgga atgccgagtt taaactgctc gccaaagccg cgaatacgct
     7621 gttcttcggc gtcatcgctc acccacagcg tgcgttgcat atcggtatcg gtggaacccg
     7681 gcgaaaccac attacagcgc acgccgctac ccgccagttc cagcccgacg ctcaacgcca
     7741 ggcttttcag cgccgctttc gatgcgccat aagcactcat accaatacgc ggagtgtgtg
     7801 cggcgtcgga cgccacagtg acaatcgccc cgccccgctg acggcgaaac tggttcatgg
     7861 tttgctggaa caggttaaac gcaccgccga cattaaccgc aaaagtctgc tgccagtcct
     7921 ccttactgag ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg accagcacgt
     7981 ccagtcgctc cgtttcagct aacagtcgct gacatacctg cgccacctgc gcagcatcgg
     8041 caacatccat tacttcggtc gcaaagggat attgctcctg agcgaacgct tgatcaaaac
     8101 ctgtaacttt cgctcccgct tcaacaaacg ccagcgccgt ggcgtagccg atacctttcc
     8161 ccgcgccggt tacccagaca tttttaccgc tgaaatccat tatttcacct cgcgggagag
     8221 tagcttccac caggcgtcga tggtcgggtt tttcgccaac atgacaaagt cgatatcgcc
     8281 atgcactttg cgccagcgcg ccgccagcgc catcatgcgc accgaatcca gaccgtagtc
     8341 gatcaggttg tcgtcatcga acggttcatc ggactcgtcc agcaacggca ggatcacctc
     8401 acgcagcgcc gctttgctgg cggggatagg tgctggcagt aattcttcgg tcatcaccac
     8461 ccggccagaa cgtccggcca catatttcag cgacatcaaa tgctcgtcac ggctgaaatc
     8521 ggccagcgca tccgccacca taaacggttt aatatcgcgc ataaatgcgt cggttgcggt
     8581 ggtcatacag ccaatgtggg catatacccc ggtaataatc agctggttac gtccactctc
     8641 tttgagcatt tgctccagcg gagaacgatg aaacgcgctg tagcgccact tcaccagcac
     8701 cgtgtcgtcg gcatctggcg tcagtcgatc caccaccttt tgctgttccg gcgaacgggt
     8761 caggcccggc ccccacatat cattcaacag cgcccgatct tcatcgctct gctctttcgg
     8821 ctgggcggtg taataaaccg ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat
     8881 attcgcgatc acctgctcca tcatcgggca gttctcgccc cagaagctga caaaatagtc
     8941 ctgcatatca tggattaaca acgcggcacg ttgcggttca aaggcccagt caactttatt
     9001 ctgcggaata tcgtgagact ccggcagtgc gtaagcctgt aattttggaa tagccatcgt
     9061 gttctctcct tcaggctgat gcgcgtgacg ccagccactg acgtaattgt tttttatcga
     9121 ctttcccgac cgccgtcagc ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa
     9181 attcggcaat accctgttca cgcaggaaac gacgcacctg caccgcgcga agcggctctt
     9241 ttaccaccag ataagcacag cttttttcgc ccatcagctc atcttccata ctgaccagtg
     9301 cggcgtagat caccgccgga tggcgcagta acaggttttc gatctcttcg gcagcgatct
     9361 tctcgccgcc acggttgatc tgatctttct cgcgcccctg cacggtgatg taaccctctg
     9421 gatcaataga gatcagatcg ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat
     9481 tgtgctgcgg gcttttgtaa tagccacgga aggtatacgg cccgcgcgtc atcagtcgtc
     9541 cgacttcccc ttgcggcagt ggatttcctt cggcatcggc aacccatact tcgtcatccg
     9601 gacacattgg gtaaccctgg gtatggataa ttttctccgc gctatcatca agacgggtat
     9661 agttcaccag cccttccgcc ataccaaaca cctgctgcaa ctggcaacca atctcagcgg
     9721 gaatacgcgc cgcaagcgtg gcagaaagac gtgcgccacc gacctgtaac agtttcagcg
     9781 aggcaagctg cgcccggctt tcgccttcgg tcagcgcctg caaccacagg ctgactgccg
     9841 gcggcaccag cgcggtaacg ttaatctgat gtttttcaat caatgggaag caaagcgtgg
     9901 cgctgggatc ggccgccaga acaaccgttc ctccggcaag aaagacgccc agcgatcccg
     9961 gcgaactcat ggcgtagtta tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg
    10021 tgaactgaca aatctcgacg ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg
    10081 ggatcagctt cggtgtaccg gtggtgccgc cggaaagctg gaaatagacc acttcatcag
    10141 caggtgacgg cgtagcagta aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat
    10201 gctcaccgct gtcgttgagt agctgcacca cgcgaatgga agaatgctct gcaacaaatg
    10261 tgttgaggaa atcatccccg ctaaacagcg catgttggcg atcggcaatc agcaatgcgg
    10321 gttcaatctg gctggcatag gcgttcagtt cactacgctg atggctgaac aacgccagca
    10381 ccggcgcaac gcccagtttc agcagcgcga aaaaggtaat gtaaagttca gcgacgttac
    10441 ccagttgtac cagcgcggtt tcaccaggtt taatgccctg acggcgtaaa ctacacgcga
    10501 ggttatccgc cgcctgattc agctcccggt aactcaactg tcgctcgccg tcgataaccg
    10561 cgatgctgtc actcgcagcg tggcgagtca gaatgtcagt cagtggcaaa tcctgccagt
    10621 agcctttttc ccgatagcga cgggcaaact cttccggcca gcgggtgaat ggaatgctca
    10681 tcctcgctcc ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg acgcctgttt
    10741 cgcgccactc acccaacggt gacgacgcag gcacaatccc cgctccggca aacagacgca
    10801 cctgattttc ccgcagcttc gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc
    10861 tgtcacacca acccacaatg ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa
    10921 taacctgggt cgcggcctga tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac
    10981 aggccagagt cagtgcgttt tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat
    11041 gccacagcgt cggcgtggta atcaattgtg gagaggaagg aacgtgtaac tcactactgc
    11101 gttcgcgcag tacctctttc atcgcctgag tcaccagttc atgttcatgg cgatcttttt
    11161 ctgacgccag cagacgatta cccgcttcgc gatcgagcac ttcatccggc tgacgacgtg
    11221 cggaaccggc taacggaatg gagctaaaac gctcaccgtc tttacgtagc agcagttccg
    11281 ggctggcccc cagcagaacg ccaccatctg ccagcgggac atggaagttg taactaaccg
    11341 ggttttgcgc aatcaaccgt tccagcaata cgccactatc aatggcggcg tcagtggtga
    11401 tatcaatcaa ccgtgacaac accactttgt cgacctgcgg cgtggcggta agtgcggcgg
    11461 cgcgggcaac catctgttca aacgtggttt gctccggaat tgcctggcgt tccaccacgt
    11521 tcagcgactg gctgcgggtg aaacggcgtg cggaagcttg tttttcctga cgggagaacg
    11581 actgccagga ttcaggaata tacagcgacg aaggctgacg tggatcgaaa ggaatcgccc
    11641 cgaccatcac cggatttttg atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt
    11701 tttgctggaa gggactgtcg ggcgaatccc cgttcacagc cggttcatcg aagcgggcga
    11761 aacatcctga cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa
    11821 gtgttgccat ggtctgctgt acttcctcag ccagtgacgt atccatatca tcctccacaa
    11881 aatgataaag gctttaataa tgattatcat ttatattttc ggtcgctaac ctaaaagcaa
    11941 acgcgcacta tgtcaactct tgaggtaacg caatctgcaa ctcggggttg catctggttg
    12001 tggttataat gaaaatgaga agcattaatt tcattaataa caggaggctg atttgtgaga
    12061 ctcgccccgc tctaccgcaa tgcccttcta ttaacaggac ttttgttttc aggaatagcc
    12121 gcagttcagg ccgccgactg gccgcgtcag attactgaca gccgtggcac tcataccctg
    12181 gaaagccagc cgcagcgtat tgtttccacc agcgtcaccc tgaccggctc actgctggcg
    12241 attgatgctc cggtgatcgc cagcggcgcg accacgccga ataaccgcgt cgcggatgac
    12301 cagggctttt tacgccagtg gagcaaggtg gcgaaagaac gcaaactgca acggctctat
    12361 atcggcgaac cgagcaccga agccgttgcc gcgcaaatgc cggatctgat tttaattagc
    12421 gcaaccggcg gggattcggc gctggcgctg tatgatcagc tttccaccat cgccccgaca
    12481 ttaatcatca attacgacga caaaagctgg caggcgctgt taacgcaact tggcgaaatt
    12541 accgggcatg agaaacaagc ggcagagcgg attgcgcagt ttgataagca actggcggcg
    12601 gcgaaagagc aaatcaaatt accgccgcag ccggtcactg ccattgtcta tactgccgct
    12661 gcacacagtg ccaatctctg gacgccggaa tcagcacaag ggcagatgct ggaacaactc
    12721 ggctttacgc tggcgaagtt acccgcaggg ttaaatgcca gccaaagcca gggtaaacgc
    12781 catgacatca ttcagcttgg tggggaaaat ctggctgcag ggttaaatgg cgagtcacta
    12841 ttcctgttcg ccggtgatca gaaagatgcc gatgctattt atgctaatcc gctgctcgcg
    12901 cacctgcctg cagtacaaaa caagcaggtt tatgcgctgg gaaccgagac gttccgtctg
    12961 gattactaca gcgccatgca agtgctggaa cgacttaagg cgctgtttta agcattaacc
    13021 gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag
    13081 caataacaac acgccgataa tcaataaacc aaaaccgctc gcgcttgcgg aagcaaccgg
    13141 tgtcatcatc gctcccaaac cacccagcag cgccgcgcct atggcatcgc ccgtcacgtt
    13201 ttgtgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt gcgtttgcag
    13261 cattgtgtat tgcagcaacg agctgaccgc actcaaccag ccgaacagcg ccagacaaac
    13321 cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga acgatcccag
    13381 cgtggagagc agcatcaata accctggtcg cgcgctatgc gccagcttcc cgctggttaa
    13441 cgcgccaata gccgcgccga gcgggatcgc cgcatagaga aaaccaatct gtgccgccga
    13501 catctgccag ttgtcagcca gcgccggata cagtacccgc accgcactcg ccatcgtcaa
    13561 taaaccaccc agcagcgcaa tccctcccac cagcgggctg gcgagtagaa aacgaaatcc
    13621 tgccagtaat gatttcaacg gatgctcacg cggctgcggt ggcggtggca gtgccggaag
    13681 gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt agttccaggc
    13741 cacaccgcca gtcgccagca gtaaaccgcc aatcatgggc gaaatcaccg accccagacg
    13801 cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta ccagtgctgg
    13861 tgtcgccgcc aatagcgccg taacgccaag cgatgcgaaa aaaccatccc ataaaccaag
    13921 taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca gtccaatgaa
    13981 gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat agcgatccgc
    14041 cagcacaccg ccgaccatca ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag
    14101 ccccacctgc caggtggaat gtgtcatcat ctggatctgc accggcaccg cgacgccgag
    14161 caaacccaga gacacaattg agatgaaacg agcgaggaat actgcgcgaa acgccgggtg
    14221 cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa tgccttgcca
    14281 tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa ataagatcga
    14341 taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt gccgtgacac
    14401 gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca ttaagcctgc
    14461 tcattggggc aaaatcactc cccgcttccg tagtgctgga ggccttctcc ggcacctgcc
    14521 agagcgccga ctgcaccatc gtgctcgacg cgcggctgcc gcgtaccctt gccggtttac
    14581 tggcaggcgg cgcgcttggc cttgccgggg cgttaatgca aaccctcacc cgaaacccac
    14641 ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt gtgctgggtg
    14701 cggcgctgtt tggttactct tccgcacagg aacaactggc gatggccttc gccggggcgc
    14761 tggtggcctc gttgattgtt gcctttaccg gcagccaggg cggtgggcag ttaagtccgg
    14821 tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg accagcggca
    14881 tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg
    14941 atattcgcaa tctacatacc ttaaaagtgg tgctgatccc agtgctgatc gccggagcaa
    15001 ctgcgctatt actgagtcgc gcgctgaaca gtttaagcct tggcagcgac accgcgacgg
    15061 cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt accgtgcttt
    15121 gtggtagtgc gacggcggta gttggcccta ttgcctttat tggcctgatg atgccgcata
    15181 tggcgcgttg gctggtgggt gccgatcatc gctggtcgct gcccgtcacg ctacttgcta
    15241 cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc ggtgaactgc
    15301 gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc gtgcgacgta
    15361 aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct gtttgctgct
    15421 gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg tcacgctgga
    15481 aatctcgcag gtattcgccg cgctgatggg cgatgcgccg cgcagtatga cgatggtggt
    15541 caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg cactgggcgt
    15601 cagcggtgcg atttttcagt cgctgatgcg taacccactc ggcagccctg acgtaatggg
    15661 ctttaacacc ggggcgtgga gcggcgtgtt ggtggcgatg gtgctgtttg gtcaggatct
    15721 gacggctatc gcgctggcag caatggtggg cggcattatc acttcgctgt tggtctggct
    15781 gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggta tcggcgttcg
    15841 cgccatgctg gtggccttta atacctggct gttgctgaaa gcgtctttag aaacggcgct
    15901 aacagcaggt ttgtggaatg ccggatcgct caacggcctg acgtgggcaa aaacctcgcc
    15961 ttccgcaccc atcattatat tgatgctaat tgccgccgcc ttactggtac gccggatgcg
    16021 cttgctggaa atgggcgatg ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg
    16081 tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc
    16141 gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg gcaccgctcg
    16201 ctgggggcta acccaggcgg cgctatgcgg ggcgctgtta ctgctggcgg ccgatctctg
    16261 cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg tcagcctcgg
    16321 cggtatttac cttatcgttt tgttaattca ggagtctcgc aaaaaatgac cgaatcagta
    16381 gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt tgcggaaaat
    16441 ctgactgtag aaatacctga tggtcacttc acggcaatta tcgggccaaa tggctgcggt
    16501 aaatccacgt tgctgcgtac cttaagccgc ctgatgacgc ctgctcatgg gcatgtctgg
    16561 ctggatggcg agcacattca acattacgcc agtaaagaag ttgcccggcg gattggtctg
    16621 ttggcgcaaa atgccaccac gctgggagat atcaccgtgc aggagctggt ggcgcgtggg
    16681 cgttatccac atcaaccgct gtttacccgt tggcgcaaag aagatgaaga ggcggtaacg
    16741 aaagcgatgc aggccacggg aataactcat cttgcagatc aaagcgtgga taccctttcc
    16801 ggtgggcaac gccagcgagc gtggatcgcg atggtgctgg cccaggaaac ggcaattatg
    16861 ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgattt gctggaactg
    16921 ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca cgatcttaat
    16981 caggcctgtc gttacgccag ccatttgatt gcattgcgag aagggaaaat tgttgctcag
    17041 ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg tctccgctgc
    17101 atgatcattg acgatccggt agccggaacg ccgctcgtgg tgccgctcgg acgaacggca
    17161 ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg ttttctggat
    17221 gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat cagcgcggaa
    17281 aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga cgggcttaac
    17341 tgatatttaa acggcgtaaa attcacatcg ttgatatttg cttttgttaa ctgctcaaca
    17401 agatactgac gattacgtaa ttcaccgttc agttccgcaa cgtcagtgac cgctttttca
    17461 atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc ggggtcatct
    17521 ttaacggcct gaccattact gtaaacgggc tttttaattc ctgccgcgtt ggcaatgtcg
    17581 agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcatttt aatgcggtcc
    17641 tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg gacgttttct
    17701 atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa aacggtctgc
    17761 gcctcttcac tggttggcgc ggtaaaactt agcgtccagg aggtatacag tgacggttca
    17821 tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt
    17881 gcgcgatgca aatccagttc gtcgattttc gcctctttta attggtccat cacataaggt
    17941 gatgaacgca ggtactcttc cagcaagcta accgactgaa acttcttgat aaacaggtta
    18001 aatgcttctg tacgatcaat tttgatatcc agatccagca cacgaagctt agtgaatgtt
    18061 ttctccaact cttgccactg aacaggttct ggaggcgtga caaccgccgc gctggtccat
    18121 ttttgcggca ggatgaaaga gatcagcaag cctgcacagg caaacgcaaa aacgaccgcc
    18181 atgaccgttt ttttggcccg ccataaaact gagattagat taagtaaatc aatttcatta
    18241 ttactgggcg acgccagagg ataatcggga aaatgagcgt cacttccctg tttaatattc
    18301 agtgatgaca tggggtataa cctgaaaatg agtccaatag ggggaaatct ccacggcgct
    18361 cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcat tccactgaat
    18421 gcaacattca ctcta
//
LOCUS       DAZGXS010000091        18429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGXS010000091 DAZGXS010000000
VERSION     DAZGXS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(83..730)
                     /gene="ychE"
                     /locus_tag="ACHKG5_003246"
     CDS             complement(83..730)
                     /gene="ychE"
                     /locus_tag="ACHKG5_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0869296.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(761..814)
                     /gene="ychE"
                     /locus_tag="ACHKG5_003247"
     CDS             complement(761..814)
                     /gene="ychE"
                     /locus_tag="ACHKG5_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0869297.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1207..3882
                     /gene="adhE"
                     /locus_tag="ACHKG5_003248"
                     /gene_synonym="adhC"
     CDS             1207..3882
                     /gene="adhE"
                     /locus_tag="ACHKG5_003248"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0869298.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4183..4800)
                     /gene="tdk"
                     /locus_tag="ACHKG5_003249"
     CDS             complement(4183..4800)
                     /gene="tdk"
                     /locus_tag="ACHKG5_003249"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0869299.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5404..5817
                     /gene="hns"
                     /locus_tag="ACHKG5_003250"
     CDS             5404..5817
                     /gene="hns"
                     /locus_tag="ACHKG5_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0869300.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5961..6869)
                     /gene="galU"
                     /locus_tag="ACHKG5_003251"
     CDS             complement(5961..6869)
                     /gene="galU"
                     /locus_tag="ACHKG5_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0869301.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7071..8084)
                     /gene="rssB"
                     /locus_tag="ACHKG5_003252"
     CDS             complement(7071..8084)
                     /gene="rssB"
                     /locus_tag="ACHKG5_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0869302.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8176..9081)
                     /gene="rssA"
                     /locus_tag="ACHKG5_003253"
     CDS             complement(8176..9081)
                     /gene="rssA"
                     /locus_tag="ACHKG5_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0869303.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9194..9652
                     /gene="ychJ"
                     /locus_tag="ACHKG5_003254"
     CDS             9194..9652
                     /gene="ychJ"
                     /locus_tag="ACHKG5_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0869304.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9702..10544
                     /gene="purU"
                     /locus_tag="ACHKG5_003255"
     CDS             9702..10544
                     /gene="purU"
                     /locus_tag="ACHKG5_003255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0869305.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10704..10788
                     /locus_tag="ACHKG5_003256"
     tRNA            10704..10788
                     /locus_tag="ACHKG5_003256"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10738..10740,aa:Tyr,seq:gta)
     gene            10833..10963
                     /locus_tag="ACHKG5_003257"
     ncRNA           10833..10963
                     /ncRNA_class="other"
                     /locus_tag="ACHKG5_003257"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10998..11082
                     /locus_tag="ACHKG5_003258"
     tRNA            10998..11082
                     /locus_tag="ACHKG5_003258"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11032..11034,aa:Tyr,seq:gta)
     gene            11131..11262
                     /locus_tag="ACHKG5_003259"
     ncRNA           11131..11262
                     /ncRNA_class="other"
                     /locus_tag="ACHKG5_003259"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11309..11440
                     /locus_tag="ACHKG5_003260"
     ncRNA           11309..11440
                     /ncRNA_class="other"
                     /locus_tag="ACHKG5_003260"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11624..12301)
                     /gene="narI"
                     /locus_tag="ACHKG5_003261"
     CDS             complement(11624..12301)
                     /gene="narI"
                     /locus_tag="ACHKG5_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0869306.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12301..13011)
                     /gene="narJ"
                     /locus_tag="ACHKG5_003262"
     CDS             complement(12301..13011)
                     /gene="narJ"
                     /locus_tag="ACHKG5_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0869307.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13008..14546)
                     /gene="narH"
                     /locus_tag="ACHKG5_003263"
     CDS             complement(13008..14546)
                     /gene="narH"
                     /locus_tag="ACHKG5_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0869308.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14543..18286)
                     /gene="narG"
                     /locus_tag="ACHKG5_003264"
     CDS             complement(14543..18286)
                     /gene="narG"
                     /locus_tag="ACHKG5_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0869309.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4652 a         4834 c         4423 g         4520 t
ORIGIN      
        1 gcttttgata actgcgcggg aacaaagtaa aaataatgac gaagtaaacg gagcatggca
       61 gctccgtttc atttgaaagg aattaattaa gcaggccggg gaaaatcccc ttaataccag
      121 taacgataaa ttcaatcccc aatgccatca gcaatagccc cataatacgc gtaatcacgt
      181 tgatgccggt ctggcgtaaa acccgtacca gccacggtgc catgcggaac aatccccaac
      241 aacataaagc gaacaatgca atagccacaa agaaaccaaa cagatagcta atgctgtgat
      301 aacgcgtacc ccagacgatg gtagaactga tcgcccctgg ccccgccatc aacggcaacg
      361 ccaatggcac cacaccaata ctttcacgta ccgcggtttc tgatttttct tgcttgttct
      421 gtttatcctc gccaagcttg ccgctgatca tcgacatcgc tatcgtcacc accaggatgc
      481 ccccggcgat acggaacgaa tcaattgata taccaaaaag ttgcagaatc gtgtcgccga
      541 gaaaaagcga gatccacaag ataatggcca cagacaggtt ggctgtaagg ttagttttgt
      601 ttcgcgctgc cgctgtctga taactggtca tgctgataaa gacgggaata atccctaccg
      661 ggttgaccag cgcaaataac ccgatgaaaa atttgaagta aacgggaaaa tcaaaaaggg
      721 tctgaatcac ggttagctcc gaagcaaaag ccggataatg ttagccataa ataaggttga
      781 aaagacgcgc tgacaatacg ccttttgaca gcatttttca cctcctaact acttaaaatt
      841 gctatcattc gttattgtta tctagttgtg caaaacatgc taatgtagcc accaaatcat
      901 actacaattt attaactgtt atctataatg gcgaaaagcg atgctgaaag gtgtcagctt
      961 tgcaaaaatt tgatttggat cacgtaatca gtacccagaa gtgagtaatc ttgcttacgc
     1021 cacttggaag tgacgcatta gagataataa ctctaatgtt taaactcttt tagtaaatca
     1081 cagtgagtgt gtgcgcgagt aagcttttga ttttcatcgg ttaagcaaat catcaccgca
     1141 ctgactatac tctcgtattc gagcagatga tttactaaaa aagtttaaca ttatcaggag
     1201 agcattatgg ctgttactaa tgtcgctgaa cttaacgcac tcgtagagcg tgtaaaaaaa
     1261 gcccagcgtg aatatgccag tttcactcaa gagcaagtag acaaaatctt ccgcgccgcc
     1321 gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa tggccgttgc cgaatccggc
     1381 atgggtatcg tcgaagataa agtgatcaaa aaccactttg cttctgaata tatctacaac
     1441 gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag acgacacttt tggtaccatc
     1501 actatcgctg aaccaatcgg tattatttgc ggtatcgttc cgaccactaa cccgacttca
     1561 acagctatct tcaaatcgct gatcagtctg aagacccgta acgccattat cttctccccg
     1621 cacccgcgtg caaaagatgc gaccaacaaa gcggctgata tcgttctgca ggctgctatc
     1681 gctgccggtg ctccgaaaga tctgatcggc tggatcgatc aaccttctgt tgaactgtct
     1741 aacgcactga tgcaccaccc agacatcaac ctgatcctcg cgactggtgg tccgggcatg
     1801 gttaaagccg catacagctc cggtaaacca gctatcggcg taggcgcggg caacactccg
     1861 gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg catctgtact gatgtccaaa
     1921 accttcgaca acggcgtaat ctgtgcttct gaacagtctg ttgttgtcgt tgactctgtt
     1981 tatgacgcag tacgtgaacg tttcgcaacc cacggcggct atctgttgca gggtaaagag
     2041 ctgaaagctg ttcaggacgt tatcctgaaa aacggtgcgc tgaacgcggc tatcgttggt
     2101 cagccagcct ataaaattgc tgaactggca ggcttctctg taccagaaaa caccaagatt
     2161 ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt tcgcacatga aaaactgtcc
     2221 ccgactctgg caatgtaccg cgctaaagat ttcgaagacg cggtagaaaa agcagagaaa
     2281 ctggttgcta tgggtggtat cggtcatacc tcttgcctgt acactgacca ggataaccaa
     2341 ccggctcgcg tttcttactt cggtcagaaa atgaaaacgg ctcgtatcct gattaacacc
     2401 ccggcttctc agggtggtat cggtgacctg tataacttca aactcgcacc ttccctgact
     2461 ctgggttgtg gttcctgggg tggtaactcc atctctgaaa acgttggtcc gaaacacctg
     2521 atcaacaaga aaaccgttgc taagcgagct gaaaacatgt tgtggcacaa acttccgaaa
     2581 tctatctact tccgccgtgg ctccctgcca atcgcgctgg atgaagtgat tactgatggc
     2641 cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca acaatggtta tgctgatcag
     2701 atcacttccg tactgaaagc agcaggcgtt gaaactgaag tcttcttcga agtagaagcg
     2761 gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg caaactcctt caaaccagac
     2821 gtgattatcg cgctgggtgg tggttccccg atggacgccg cgaagatcat gtgggttatg
     2881 tacgaacatc cggaaactca cttcgaagag ctggcgctgc gctttatgga tatccgtaaa
     2941 cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa tgattgctgt caccaccact
     3001 tctggtacag gttctgaagt cactccgttt gcggttgtaa ctgacgacgc tactggtcag
     3061 aaatacccgc tggcggacta tgcgctgacc ccggatatgg cgattgtcga cgccaacctg
     3121 gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc tggacgcagt aactcacgcc
     3181 atggaagctt atgtttctgt actggcatct gagttctctg atggtcaggc tctgcaggca
     3241 ctgaaactgc tgaaagaata tctgccagcg tcctaccacg aagggtctaa aaatccggta
     3301 gcgcgtgaac gtgttcacag tgcagcgact atcgcgggta tcgcgtttgc gaacgccttc
     3361 ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc agttccatat tccgcacggt
     3421 ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca atgcgaacga caacccgacc
     3481 aagcagactg cattcagcca gtatgaccgt ccgcaggctc gccgtcgtta tgctgaaatt
     3541 gccgaccacc tgggtctgag cgcaccgggc gaccgtactg ctgctaagat cgagaaactg
     3601 ctggcatggc tggaaacgct gaaggctgaa ctgggtattc cgaaatctat ccgtgaagct
     3661 ggcgttcagg aagcagactt cctggcgaac gtggataaac tgtctgaaga tgcattcgat
     3721 gaccagtgca ctggcgctaa cccgcgttac ccgctgatct ccgagctgaa acagattctg
     3781 ctggatacct actacggtcg tgattatgta gaaggtgaaa ctgcagcgaa aaaagaagcc
     3841 gctccggcta aagctgagaa aaaagcgaaa aaatccgctt aatcagtagc gctgtctggc
     3901 aacataaacg gccccttctg gggccgtttt tttgtttacc caaagcaact tttccataaa
     3961 ccgacagcat tagccttcat catatttgcg acgatgtata acgcctaaac acagggatat
     4021 tgtactttac aggtcacaag tcaacgtcgg tgcttaagag ccctgtgagg cgtatagcgg
     4081 cgttaaaaac tgccgagaag ggtgtatagc ccggaagaag tgcgtaaaac gaactgacag
     4141 gataaaagtg ccctgctcac cctgtcagta aagaaattct tattaatcgt ggcgatgcct
     4201 ttcctgaata gccgttaatg agccgacttg taacgcctct ttatagtgtt tacggcatac
     4261 agaaacgtat cgttcattac caccaataac cacctgctca ccttcgttat aaggtctgcc
     4321 tgcttgatca agacgcagca ccatgcttgc tttacggcca caaaaacaga tggtttttaa
     4381 ttcaaccagt ttgtcggacc atgccagtaa gtattggctg ccaataaata attcacctcg
     4441 aaaatcggta cgtaaaccat aacaaagtac gggtatatcg agttgatcga caacctccga
     4501 taattcatat acttgttgtc tggttaaaaa ctggcattca tcaaccagta cgcaatgaat
     4561 tgcctgctgc tcatgttccg cacgaatctc atcaaataat gatgaatttt ggttaaataa
     4621 ttttgcaggc gatgacaaac ctatacgcga actgactttc ccggcaccaa agcgatcatc
     4681 aatttctgcc gtatatacga cagtgcgcat gccgcgttcc tggtaattgt atgaagattg
     4741 caacaatgct gtagacttac ccgcattcat tgcggaatag tagaaatata gctgtgccat
     4801 cagccacagg ccctcaatga tatgaaataa agttggctgg agtttatcat aattcgtcag
     4861 tttttcagct caacagtatg cacagaagtg gtatatgcgt tctcccttac gaagccttgc
     4921 ataatccttc tgagctatca ttacaactgc ctcgcttgtt ataagcgggt aaatgactgc
     4981 tggtaactat tcacaatctt taacctgttg cgcaagtaat agccctctgt tgacctccag
     5041 gagatagtgc aatactaagt ccctgctctt attgcgactg ttctactttt catcattcgc
     5101 ttaataggga attctcgtaa acacgactaa tacagaagac tgaaaggtcg tcagcctacg
     5161 ataatctccc cataaaatgt gacatgaatc aggaagtttt aacctcacgt gctgcgaaat
     5221 catcggtaca aatagggcta tatgccgcgt cttttctggc taattttatg aaaagatatt
     5281 tattggcggc acaaaataaa gaacaatttt gaattcctta cattcctggc tattgcacaa
     5341 ctgaatttaa ggctctatta ttacctcaac aaaccacccc aatataagtt tgagattact
     5401 acaatgagcg aagcacttaa aattctgaac aacatccgta ctcttcgtgc gcaggcaaga
     5461 gaatgtacac ttgaaacgct ggaagaaatg ctggaaaaat tagaagttgt tgttaacgaa
     5521 cgtcgcgaag aagaaagcgc ggctgctgct gaagttgaag agcgcactcg taaactgcag
     5581 caatatcgcg aaatgctgat cgctgacggt attgacccga acgagctgct gaatagcctt
     5641 gccgccgtta aatctggcac caaagctaaa cgtgctcagc gtccggcaaa atatagctac
     5701 gttgacgaaa acggcgaaac taaaacctgg actggccagg gccgtactcc agctgtaatc
     5761 aaaaaagcaa tggatgagca aggtaaatcc ctcgacgatt tcctgatcaa gcaataatct
     5821 tttgtagatt gcacttgctt aaaatcccgc catcggcggg attttttatt gtccggttta
     5881 agacaattta ataagaataa gctataaaaa aacggcgtcg attgctcaac gccgtttcgt
     5941 ggataacacc gatacggatg ttacttctta atgcccatct cttcttcaag ccaggcttta
     6001 aattccgtgc caagggtgtt atgacgaata ccgtattcaa cgaaggcctg catgtaacct
     6061 aatttattac cgcagtcatg gctcttccct ttcatatgat aggcttcaac cgtttctttt
     6121 tcgattagca tatcaattgc gtcggtgagc tgaatttcat caccagctcc cggaggggtt
     6181 tttgccagca acggccaaat atccgcacta agtacgtaac gacccacaat agcgagatta
     6241 gacggcgcaa catccgcttt tggtttttca accacgccaa ccatcggtac gctttcaccc
     6301 ggcgctaatt caacgccttt gcaatccaca acgccatatg cggtcacatc agcaaccggt
     6361 tcaaccatga tctggctatg acccgtttca tcgaagcggc ggatcatctc tgccaggtta
     6421 tcctgtgaca aatcggattc atattcatcc agaataacgt caggcaaaat aacagccaca
     6481 ggttcatcac ccactaccgg gtgagcacac aataccgcgt gtcccaggcc tttcgccagc
     6541 ccctgacgaa cttgcataat agttacgtgc ggcggacaaa tagactgcac ttcatcaagc
     6601 agttgacgtt ttacacgttt ttccagcatt gcttccagtt caaaactggt atcaaagtgg
     6661 ttttcaatag agtttttaga tgagtgtgta accagcacaa tttcagtaat gccagccgca
     6721 atacattcat tcacgacgta ttgaattaat ggcttatcga caagtggcag catctctttc
     6781 gggatggctt tcgtcgccgg caacatcctg gttcctaatc ccgcaacggg gataacggct
     6841 tttttgactt tcgtattaat ggcagccatt taaattctcc tggactgttc gtgttttgaa
     6901 cgtgttcata tttctgtatc gcatcccagt atatcagcac cccggagggg ctaagttctg
     6961 aaaaggatgc gagttagcat aattaagaca attacttata cgaatggcga cgatagtacc
     7021 accagccgac attagctggt aatgcaaatt tagcccgcgt tatcgtttgc tcattctgca
     7081 gacaacatca agcgcagtcg accaccggtt ccccatattt ggcattgcca ggcatcgcat
     7141 cgctggctca attgattcaa ataagcgtta cctaaagtgc ctaacggaac accattactg
     7201 atttgcacct ggtgttcgcc ggtatttaac gtcgcattca gacccgcaga aaccagaatg
     7261 agatttttca gttcgcgatg ataatagcca actaatagcg gaaactgccc cggcagattg
     7321 gcctgacgga gtaaatggtt tacctgcttc aataacgcgc ccaactctgg caaccgttga
     7381 ttttggtgtg caagctgttc ctgtaataat ccgttaaaca atgcgcgtaa taacaaggca
     7441 gcaagtacgc cattatgtcc agctcgggtt acatcaaggc aataaaatgc cagatcgttt
     7501 tccgaaagtg cggcaatatc aagcaccagg ccgggtttgt ccgcggcaac caattgacgg
     7561 taattaaccc ggcaatggga aatcacctgc tgaaccggcg gttgtagttc ctgtaataat
     7621 ttcgccgctg cggcagggtt atcaaccatt gcatcccagt cgcgaaaaag cctttcctct
     7681 tcctcaacgc gcgaattaaa catgctggga tagagacagg caaaaaccat ctcgcgcaag
     7741 cgattcagat ctttaactgg tttcagcaaa acatcttcaa cgcccagacg taacgctttg
     7801 gcaatatctg ccatattttc agtggcagat atcaccagaa ctggggtctg gtcgcctctg
     7861 ttacgtatat gctccagcag tttaagcccg ttcattcgtg gcatcgcgat atcacatatc
     7921 atcaggtctg gagtgaaacc tcccagcaac tcaagggcat ccaccccatc agccgccagt
     7981 accgttgtcg ctcccaatga ggaaaaccac gaatccagaa gcgagcgaaa tacctgctca
     8041 tcttcaacaa tgagaatctg ttttccgacc aatggctgcg tcatgttctc tcccctgact
     8101 ggctttactc aatagtggca tgctattgcc acctgcgcct gtcagaatta acttaagtgt
     8161 agataaaaat tctggtcaaa tgttggtgcg taccaacggc aaaagttcgt ccattttctt
     8221 ttccactgcc agctgcccgg ccgcaatggc agcgtgcgcg cgatggaaat caagcgtaga
     8281 tatttgcggg caaacaggtt gaatcagaat atcgggcgga tcacctgcca tgcggttcct
     8341 tttaaggcgg ttctccagca cctggataga agtggtcata atctctgttg ccgttggtgc
     8401 tgtcaccgcc cgacgtgtcg ttatactgcc taacctttct ttcagacgcg catgccacgg
     8461 cagagaatca ccattctcgc tattttcttc actgacatta aaggagagca aatcttgttg
     8521 catcaaatga gcatcgtgct gcaggtcgac cgctatcaca atatcagcac ccaatgcacg
     8581 cgtgagcgaa ataggaattg ggttaacgac tgccccatca accagccagt agccgttatg
     8641 cgcaacgggt gccattagtc ctggaatact gcatgatgcg cgaatagcaa gatggagatc
     8701 gccttcagta aaccataatt cacgcccagt acttaaattg gtggcaacag ccgcgaagcg
     8761 acgggaacaa ttttcgatct ctgtttccgg cattatttcg cggtattgat tgaagacacg
     8821 ctcgccgcgc agtaacccac cgcgctgcca ggagagatcc atcagtcgta aaacatccca
     8881 ataactgaaa gaggtcaccc aatcttccag cgcagataat cgatcgcatg cataggccgc
     8941 gcccaccagc gaaccaattg aacatcctgc aacgatatca atttcaatac ccactttttt
     9001 tagcgcatta ataacgccaa tatgcgacca acctctcgcc gcgccagatc ccagcgccag
     9061 ccctattttt atctttctca ttattcccgc aagattcccc tggaatgcaa tcgtagccac
     9121 atcgctactg ctcggttaac atagtgctac tcaggcggtt aatacgccgt tattgtttcc
     9181 agggagatta tttgtgtctc agctttgtcc ctgtggtagt gctgtcgagt atagcctatg
     9241 ttgccaccct tatgtgtctg gtgaaaaggt tgcacctgat ccagaacatc tcatgcgttc
     9301 gcgttactgc gcttttgtga tgcaagacgc agattattta ataaagacct ggcatccatc
     9361 ttgtggagca gcggcgttac gtgccgaatt gattgccgga tttgcgcata ctgaatggtt
     9421 gggattgacc gtatttgaac attgttggca ggacgccgac aatatcggat ttgtcagttt
     9481 tgttgctcga tttaccgaag gtggcaaaac aggtgcaata attgaacgtt cgcgattttt
     9541 gaaagaaaac ggtcaatggt actatattga cggtacacgt ccgcagtttg gccgcaacga
     9601 tccctgccct tgtggttcag gtaaaaaatt taaaaagtgc tgcggccaat aatggttgac
     9661 ggtacggttt agcaaacact ctcaacaagg tttttccagc aatgcattca ctccaacgta
     9721 aagttctgcg tactatttgt ccggaccaaa aaggtctgat cgcacgtatt accaatattt
     9781 gctacaagca cgagttaaat atcgtacaga acaatgaatt tgttgatcac cgtaccgggc
     9841 gcttttttat gcgcacggaa ctggaaggga tttttaatga ttccaccctg ctggcggatc
     9901 tcgatagcgc attgccagaa ggctccgtgc gtgagctgaa tcctgccggt cgtcgccgga
     9961 tagtgattct ggtcactaaa gaagcgcatt gccttggcga tttgttgatg aaagccaatt
    10021 atggcggcct ggatgtcgaa atcgcggcag tgattggtaa ccacgatact ttacgttctc
    10081 tggttgagcg ttttgatata ccgtttgagc tggtaagcca tgaagggtta agccgcaacg
    10141 agcacgatca aaagatggcg gatgccattg atgcttatca acctgactac gtggtgctgg
    10201 cgaagtatat gcgggtatta acaccggaat ttgtgtcacg cttcccgaat aagatcatca
    10261 atattcacca ttccttcctg ccagcgttta tcggcgcacg tccttatcac caggcctatg
    10321 aacgtggcgt gaagattatt ggcgcaaccg ctcactatgt gaatgacaat ctggacgaag
    10381 gcccaatcat catgcaggac gttattcatg tcgatcatac ctacacagct gaagatatga
    10441 tgcgcgcagg tcgtgacgtc gagaaaaacg tcttaagtcg cgcgctctac aaagtactgg
    10501 cgcagcgcgt ctttgtttac ggtaatcgga cgattattct ttaatcgcca gcaaaaataa
    10561 ctggttacct ttaatccgtt acggatgaaa attacgcaac cagttcattt ttctcaacgt
    10621 aacactttac agcggcgcgt catttgatat gatgcgcccc gcttcccgat aagggagcag
    10681 gccagtaaaa agcattaccc cgtggtgggg ttcccgagcg gccaaaggga gcagactgta
    10741 aatctgccgt catcgacttc gaaggttcga atccttcccc caccaccatg attcaccaca
    10801 gcgatgtggt aacttcggtg agattgtgaa agtttgcact catcggggaa gggtgagaac
    10861 cttcgactaa ggttcgactc gagcgaaagc gagagaacgt tttcacaggc aacggcccga
    10921 agggtgagga gcgaagcgac gaataatcct ttccccaccg ccatctcaag cattacctca
    10981 aaatttgaat tacccctggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg
    11041 ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttt tcaaaagtcc
    11101 ctgaactctc aagcgaatcc gtaaataaat attctgccca agcggagaag gatgagaagc
    11161 ttcgactaag gttcgactcg agcgccagcg agagagcgtt gccgcaggca acgacctgaa
    11221 gggcgaagcg cgcagcgctg agtaatcctt cccccaccac catcactttc aaaagtccct
    11281 gaactctcaa acgaatccgt aattaaatat tctgcccaag cggggaagga tgagaagctt
    11341 cgaccaaggt tcgacttgag cgccagcgag agagcgttgc cgcaggcaac gacccgaagg
    11401 gcgaagcgcg cagcgctgag taatccttcc cccaccacca tcgcttcctg aaataatcaa
    11461 aacctgttac tccgagctaa ttttgcccaa tggcaccacg tttaccggac ctacaatatc
    11521 ctcccttgta ggtcggataa gcgaagcgca tccgacaact caccccgcac cgtgcccata
    11581 aaaaaacccc gccgaagcag ggtctatgtg aactaaaatt cgcttagtga cgagcgcgca
    11641 ccagctggta cttacgtgtc agatactcca ccggtacgct ccagatgtgt accagacgcg
    11701 agaacgggaa cagcaggaat aacgtcatcc ccagcaccag gtgtagacgg aagataaacg
    11761 ccacaccatc gaggtgttga gaagcgccac cgtggaaggt cactaccgac tgcgcccagc
    11821 caaccagttt catcatctcg ctaccgtcca tatgttgggc ggagaacgga atggtcaaca
    11881 ggcccagcgc gcactggata acgagcagcg acaggatcag tatatccgct ccggtagtcg
    11941 ttgcacgcac gcgtgggctg aacagacgac gtttaagcaa aagcacgccg ccaatcagac
    12001 acagcacgcc gctggcacca ccagcaaaca tcgccatttt ctgtttcact tcaatcggca
    12061 gccaggcttc atacatccag tgcggcgtca acataccgaa gaagtgaccg acaaaaatcc
    12121 ccagaatacc gatatggaac aggttcgacg ccaggttcat ccctttgcga tccagcatct
    12181 ggctggacgc cgcgcgccag gtgtactgcc cgtagtcata acgcagccag ctaccaatca
    12241 ggaagaccgc cccggctatg tacgggtaga tatcaaagaa gaacatattc aggaattgca
    12301 ttagtgctgt cctccggtgg tgatattcag atattgcggc gcgacggcac cggcaaagcg
    12361 acgctgatga gcagtgattg ctgaatcacc gcagcctttg tcagcaaaga atttaacctg
    12421 ctcttcttcc caaacagcat ccagcgcctg cggcgtatca tcgcgcgctt cgtcggcaat
    12481 tttttccgcc actttgtcgc tgtcgatagc ggtattggcc aatttcagca gcagatcaaa
    12541 cagcacggca taacggcttt cacgctgttg cagacgcgca ctcagcaatg ccagaatcgg
    12601 cgcgatatct ttcaaacctt ccacggcttc gctttgcggc agctgcgcca ggtactccag
    12661 atacagcggc agatgatccg gcaattcgcg gctgtttaac tgcaagccgt gctgctcgta
    12721 ctgcgccagc aggtccacca tcgcctggcc gcggtcgcgg gattcgccgt gcacatgttc
    12781 aaacagcaac agtgacgtgg cgcggccacg gtcgaacagt tcgctgtact gcgcctgggc
    12841 atcgagcgga tccatcgtcg ttaaatcgcg caggaaaatg cccagcgcat gggcatcctc
    12901 ttttggcaga tttttcgacg cggcaatcgc ctcaaacatc tcttgttgat gctgccataa
    12961 ggcagcatcc ggatattcaa ggagacgcga tacaatcacg agttcgatca tggatgcggc
    13021 tccgttttgc tggtcacatc gatggcatcg atacgacggc tgttgaacag attgaatttg
    13081 gtatctgaac cgtggcagcc atcaccaaag gtaaagccgc agccattttt ctccgggaag
    13141 gcttcccgtg ccagttcacg atgactactc ggcaccacaa agcgatcttc gtagttagca
    13201 atcgccagat aacggtacat ctcctgtgcc tgggcttcgg tcagaccgac ctcttccagc
    13261 gcacgggtat caactttacc gtcaacggtt tcagcacgtt tgtaatgacg catcgccagc
    13321 atacgtttca gcgcgcgcag taccggtttg gtatcaccgg cggtcagcag attcgccaga
    13381 tactgtaccg gaatacgcag gctttcgacg tctggcagaa tgccgttgct acccagctca
    13441 cccgcgtctg ctgcagactg aatcggagag agaggcggca cgtaccagac catcggcagt
    13501 gtgcgatatt ctggatgcag cggcagcgcc agtttccatt ccattgccat tttataaacc
    13561 ggcgactgct gtgcagcttc aataacgctc agcggaatac cgtctttaat cgcctgctcg
    13621 atgactttcg gatcgttcgg atcgaggaac acctccagct gacgctggta gagatctttc
    13681 tcgttctcgg tgctggctgc acgttcaatc gcgtcggcat cgtacagcag cacgccaaga
    13741 taacggatac gaccgacaca ggtttctgag cacacggtcg gctgacccgc ttcaatacgc
    13801 ggatagcaga agatgcactt ctcagactta ccgctcttcc agttgaagta gatttttttg
    13861 tacgggcatc cggtgatgca catacgccag ccacggcatt tatcctggtc gatcaggacg
    13921 atgccatctt cttcacgctt gtaaatcgca ccgctcgggc aggtcgccac acatgccggg
    13981 ttcaggcagt gttcgcacag gcgcggcaaa tacatcatga aggtgttttc gaactggcta
    14041 tacatcgcct tctggatgtt gtcgaagttc ttgtctttcg ccagtttgtc aaactcaccg
    14101 cccaggtcat cttcccagtt cggccctttt tcgattttcg ccatccgttc cccggtaatc
    14161 agcgaacgcg gacgggcaat cggctgcgat ttgctgcctt ccggcgcggt atgcaggttc
    14221 tgatagtcaa aatcgaacgg ctcgtaataa tcgtcgatcc ccggcagatg cgggttagcg
    14281 aagattttac ccagcagcat ggcacggtta cccatgcgcg gttgcagttt gccgttgatt
    14341 ttacggatcc agccgccttt gtatttttcc tggttttccc agtcagtcgg gaagccctgg
    14401 cccggcttgg tttccacgtt gttgaaccac gcgtattcca cgccttcacg gctggtccag
    14461 acgtttttac aggtaactga acaggtgtgg cagccgatgc acttatcgag attcagcacc
    14521 atgccgactt gtgaacgaat tttcatttta cgctctcctg tacctgatca ttgccttcgc
    14581 catctaacca gtcaatgttc ttcatcttac gcactacaac aaactcatcg cggttagaac
    14641 ctacggtgcc gtagtagtta aagccgtatg ccagatgggc atagccgccg atcatatgcg
    14701 tcggtttcgg cgtgatacgg gtgaccgagt tatggatacc accacgctgt tgggtaattt
    14761 ccgaaccagg caggttaacg atacgttcct gcgcgtggta catcatggtc atccctgccg
    14821 gaacacgctg gctgacaacc gcacgggcag tcagagcacc gttgctgttg aatacttcaa
    14881 tccagtcgtt atcggcgata cccagatctt tggcatcggc ttcactcaac cagaccaccg
    14941 gaccaccgcg acctaaagtc agcatcagca ggttgtcgct gtaggtggag tggatacccc
    15001 acttctggtg cggcgtcagg aagttgagcg ctttttccgg gttgccgttg gatttctggc
    15061 ctatcacttc tttcaccgaa cgggtgtcga tcggcggacg ataaaccagc aggctttcac
    15121 cgaaatcacg catccactgg tgatcctgat acagttgctg acgaccagag agcgtacgcc
    15181 atgggatcag ctcgtgaacg ttggtgtaac cggcgttgta agaaacgtgt tcatcttcca
    15241 gaccagacca ggtcgggctg gagataattt tgcgcggctg tgcctgaata tcgcggaagc
    15301 ggatcttctc gtcttcttta ttcagcgcca gatgcgtatg gtcacgaccg gtaaattcgc
    15361 tcagggcagc ccaggctttc acggctacct gaccgttggt ttccggtgcc agtgtcagga
    15421 tcatctctgc cgcatcaatt gcggtgttca gcatcggctg gcctttcgcc ggaccttccg
    15481 ctttggtgta gttgagctta cgcagcagat ccatctcgct ctgggtgttc caggcaatcc
    15541 ctttaccgcc gttaccgatt ttctccatca gcgggccgat agaggtaaag cgttcgtaag
    15601 ttgccggata atcgcgctct acgaccataa tgtgcggtgc agttttgcct gggatcaggt
    15661 cgcattcgcc ttttttccag tctttcacat ccagcggctg cgccagttca gcggcagagt
    15721 cgtgctggat aggcagcgtg acgatgtcgg tttctttacc cagatggccg acgcacactt
    15781 cggagaattt cttcgcgatg gctttgtaga tttcccagtc gcttttcgct tcccaggccg
    15841 gatcgaccgc cgcagacagc gggtgaataa acggatgcat atccgaagta ttcatgtcgt
    15901 ctttttcgta ccaggtcgcc gtcggcaaaa tgatgtcgga atagagacag gtgctcgaca
    15961 gacggaagtc cagcgtaacc accagatcca gcttgccttc cagaccattg tcctgccagt
    16021 ccacttcttc cggcttcacg ccgccctgct gccccagatc tttaccctgg ataccgtgct
    16081 ccgtccccag caggtacttg agcataaact catgaccttt accggaagaa ccgagcaggt
    16141 tagaacgcca gataaacagg ttacgcgggt ggtttttgcc gttttccggc tgttccgcag
    16201 cgaaacggat agaaccctct ttcagggatt tcaccgtata gtccaccgga ttcatcccgg
    16261 ctttttctgc ttcgccagcg atagtcagcg ggttagtgcc taactgcggc gcagacggca
    16321 gccagcccat gcgttctgca cggacgttaa agtcgatcag atgtccggta tagcgggatt
    16381 tatccgccat cggcgacagc agttcttccg cagtcacggt ttcataacgc cactggctgg
    16441 agtggttata gaaataagag gtgctgttca tgtgacgcgc cggacgctgc cagtcgaggg
    16501 caaacgccag cggctgccag ccggtttgcg gacgcagttt ttcctgacct acatagtgcg
    16561 cccagccgcc cccgctctga ccgacacagc cgcagaaaat cagcatgttg atcagaccac
    16621 gatagttcat atcgaggtga taccagtggt tcagccccgc accgacgata atcatcgaac
    16681 gaccgtgcgt tttatcagcg ttatcggcaa attcacgggc gatgcgaata atctggctgc
    16741 gagaaacgcc ggtaatctgc tcggcccagg ccggggtata agctttcaca tcgtcatagc
    16801 tggttgcaca gttaacgtcg ttcaggccac gttccagacc gtagtttgcc agcgtcagat
    16861 cataaacggt ggtcaccagg gcggtgctgc catcagccag ttgcaggcgt ttcaccggca
    16921 gtttgtgcag cagcacgttt tccagttcca ctttgttgaa gtgttccgtg ccgtcgccac
    16981 caaagtacgg gaagcctacc tcagcgatct catcctggct acccagcagg ctcagttgca
    17041 gctcggtttc ttcgccagtt ttgccgtcgc gctgctcaag attccatttg cccttctcgc
    17101 cccagcggaa gccaatagaa ccgttcggcg caaccatttc gccattggta ttaaaggcga
    17161 cagttttcca ttccggattg ttttcctggc ccagcgcatc aaccagatca gcagcgcgca
    17221 gcatacgacc tgcagcgtag taaccgtcgc gttcttccag catcaccagc atcggcatgt
    17281 cggtgtagcg acgcacatag tcggtgaaat actggcttgg gttgtcgaga tggaattcac
    17341 gcagcattac gtggcccatc gccagcgcca ttgccgcatc ggtgccctgt ttcggtgcca
    17401 gccacagatc gcacagtttg gcgatttcag cgtagtctgg tgtgacggca acagttttgg
    17461 tgcctttgta acgcacttca gtaaagaagt gagcatctgg ggtacgcgtc tgcggcacgt
    17521 ttgaacccca ggcgatgatg tagctggagt tgtaccagtc agcagattcc ggtacgtcag
    17581 tttgctcgcc ccaggtttgc ggagacgcag gaggcaggtc gcagtaccag tcgtagaagc
    17641 ttaagcaagt accgccaatc agcgagagat agcgtgcacc cgatgcgtaa gaaaccatcg
    17701 acattgccgg aattggcgag aaaccagcaa cacggtccgg gccgtagttt ttgatggtgt
    17761 aaacgttaga tgcggcgatc agttcgttca cctcctgcca ggaagaacga acaaaaccac
    17821 cgcgtccacg cgcctgctta aagcttttcg ctttatcggc gtcttcaatg atagaagccc
    17881 atgcctcaac cggatcgcta tgcagcgcct tcgcttcacg ccacattttc atcaggcgtt
    17941 tgcgcatcat cgggtatttc aggcggttgg cactgtaaag ataccaggag tagctggcac
    18001 cgcgcgggca gccgcgaggt tcatggtttg gcagatccgg acgggtacgc ggatagtcag
    18061 tctgctgggt ttcccaggtg accagaccgt ttttgacgta gattttccag ctgcaggagc
    18121 cggtgcagtt taccccgtgg gtagagcgga cgattttgtc atgctgccaa cgctggcgat
    18181 atccatcctc ccagtcacgg ttggtattga gaagctggcc atgcccatcg gcaaaggttt
    18241 cacccttctg tttgaagtag cgaaaccggt ccaggaattt actcatcggt tttctcctgt
    18301 gggagcctgt cggccctctg ataaatcgac attgctaatg tttaagggca aggtaacgct
    18361 ctgaaacagc gtgggaattg ataacgatca agagtgatgg ggatgaaaaa taaagtaaat
    18421 tcctaagat
//
LOCUS       DAZGXS010000092        18419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGXS010000092 DAZGXS010000000
VERSION     DAZGXS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..621
                     /locus_tag="ACHKG5_003265"
                     /pseudo
     CDS             1..621
                     /locus_tag="ACHKG5_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            724..1227
                     /locus_tag="ACHKG5_003266"
     CDS             724..1227
                     /locus_tag="ACHKG5_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0869310.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1294..2751)
                     /gene="nuoN"
                     /locus_tag="ACHKG5_003267"
     CDS             complement(1294..2751)
                     /gene="nuoN"
                     /locus_tag="ACHKG5_003267"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0869311.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2758..4287)
                     /gene="nuoM"
                     /locus_tag="ACHKG5_003268"
     CDS             complement(2758..4287)
                     /gene="nuoM"
                     /locus_tag="ACHKG5_003268"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0869312.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4518..6359)
                     /gene="nuoL"
                     /locus_tag="ACHKG5_003269"
     CDS             complement(4518..6359)
                     /gene="nuoL"
                     /locus_tag="ACHKG5_003269"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0869313.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6356..6658)
                     /gene="nuoK"
                     /locus_tag="ACHKG5_003270"
     CDS             complement(6356..6658)
                     /gene="nuoK"
                     /locus_tag="ACHKG5_003270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0869314.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6655..7209)
                     /gene="nuoJ"
                     /locus_tag="ACHKG5_003271"
     CDS             complement(6655..7209)
                     /gene="nuoJ"
                     /locus_tag="ACHKG5_003271"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0869315.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7221..7763)
                     /gene="nuoI"
                     /locus_tag="ACHKG5_003272"
     CDS             complement(7221..7763)
                     /gene="nuoI"
                     /locus_tag="ACHKG5_003272"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0869316.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7778..8755)
                     /gene="nuoH"
                     /locus_tag="ACHKG5_003273"
     CDS             complement(7778..8755)
                     /gene="nuoH"
                     /locus_tag="ACHKG5_003273"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0869317.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8752..11478)
                     /gene="nuoG"
                     /locus_tag="ACHKG5_003274"
     CDS             complement(8752..11478)
                     /gene="nuoG"
                     /locus_tag="ACHKG5_003274"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0869318.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11531..12868)
                     /gene="nuoF"
                     /locus_tag="ACHKG5_003275"
     CDS             complement(11531..12868)
                     /gene="nuoF"
                     /locus_tag="ACHKG5_003275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0869319.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12865..13365)
                     /gene="nuoE"
                     /locus_tag="ACHKG5_003276"
     CDS             complement(12865..13365)
                     /gene="nuoE"
                     /locus_tag="ACHKG5_003276"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0869320.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13368..15170)
                     /gene="nuoC"
                     /locus_tag="ACHKG5_003277"
     CDS             complement(13368..15170)
                     /gene="nuoC"
                     /locus_tag="ACHKG5_003277"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0869321.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15264..15926)
                     /gene="nuoB"
                     /locus_tag="ACHKG5_003278"
     CDS             complement(15264..15926)
                     /gene="nuoB"
                     /locus_tag="ACHKG5_003278"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0869322.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15942..16385)
                     /gene="nuoA"
                     /locus_tag="ACHKG5_003279"
     CDS             complement(15942..16385)
                     /gene="nuoA"
                     /locus_tag="ACHKG5_003279"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0869323.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17016..17954)
                     /gene="lrhA"
                     /locus_tag="ACHKG5_003280"
     CDS             complement(17016..17954)
                     /gene="lrhA"
                     /locus_tag="ACHKG5_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0869324.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4589 a         5125 c         4738 g         3967 t
ORIGIN      
        1 gttatattat ttctgttttc actccctctg attatttttt accaaccctg ggtcactgca
       61 ttatcgccaa cgccccgaca cgtaattcct gagcaattag aaaaaacagt tcgttatctt
      121 acacaaactg tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag
      181 tatataaaaa aaggtctttg tcagtagcgg tgccagagtt accgcgcagg gcgtccccaa
      241 ttggctatta cgactctgcg cctggtagcc aggattaccc ttacccgcaa tgtcctggct
      301 ttatcccgat cggggggact ttattgcctg gtcggcagaa tacaggatat caacgccgtt
      361 cgtcaggtaa aaacggcatt gttatcatcc cgggatttat ctgtttattc tatgaatgcc
      421 ccatggttta ttcacggtat tgatttctct gaccacctta attattggca acacgatatt
      481 ctcgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt
      541 gatacagcag acagattgaa ttatcagaaa atggctcaga tggtggatgg tgttataact
      601 ttgttacaca acagtaaata ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt
      661 acattttatt ttccctcgtc tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa
      721 taaatgggaa tgattggtta ttttgcagaa attgactcag aaaagattaa ccaactgctt
      781 gagtctactg aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga
      841 ctggatattg ataaacgctg ggatttttta cactttgggc tgacaggcac ctctgcgttc
      901 gatccagcga agaatgatcc gctgagtcgt gccgtgcttg gtgaacacag cctcgaagat
      961 ggtatagatg gtttcctggg attgacatgg aatcaggagt tagccgcgac aatcgaccga
     1021 ctggaatcac tggatcgaag tgagctgcgt aaaaagtttt ccattaagcg actcaatgaa
     1081 atggagattt atccgggagt gaccttttca gaagagttag aggggcaact ctttgcttcg
     1141 atcatgcttg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat
     1201 catgcgctga cggttattgt tgggtgatgg tgtcagggca ccaaaaaaaa ccgccgaaca
     1261 tgtcggcggc tttctgactt acaaactgat agcttacatc agcggcattg ctaaccgcac
     1321 aatactaatc agcggttgtg gccatacacc cagcaccagt accaacagtg cagaaatcag
     1381 caccacgata ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc
     1441 cggtgcgtgc aggtacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga
     1501 accgacaacc acggcaccca ccagccacca caggtgtgcc tggacaccaa ccgccagcac
     1561 gtagaactta ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac
     1621 cgtcatcacc gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga
     1681 atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag
     1741 gctgctgaac agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccagt
     1801 ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag
     1861 cagacgtttg atattggtct ggctcagcgc catcaggtta ccgaagatga tggaggcaaa
     1921 ggcgataatc gccagcacca cccgaatcgc ttcgctgtca cccaccggtg cgtagaggaa
     1981 cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac
     2041 cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag
     2101 tttgaagccg aggccaacaa tcatcaggcc gaaacctgcc agcaacagcg gctcgtttag
     2161 cataccgtcg ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata
     2221 caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt
     2281 gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg
     2341 caaagagatc agttcaatac cgaggaacag agacgccaga tggtttgcat tcgccagcag
     2401 gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata
     2461 gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc
     2521 ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc
     2581 ctggccaaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc
     2641 gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc
     2701 gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta
     2761 cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg
     2821 cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa
     2881 caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct
     2941 tttcgcttta ccgaagtaag cgcgatgtaa catcgccagc gaataaacag atgcaaagac
     3001 cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat
     3061 cataaattcg ccgacgaagt taccggtgcc tggcatcccg agcgttgcca ccgcaaagaa
     3121 cagtgacaag gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc
     3181 gcgggtatgg atacgttcat aaagctgacc acaaagaata aacagacccg ccgctgacaa
     3241 gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat
     3301 agcaatcagt acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc
     3361 ggtctgggcg aaggccatcc acgcaccgta gaagatgccg ataacaccca gccacatggc
     3421 gattggcgcg aactctgccg acgcgtttgg gaacagcggt agggagaaac gcagcagacc
     3481 gtaagcggcg gttttcagca agatccccgc gaggtccacg gaaccggcgg tcggtgcctg
     3541 tgagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttca ccgcaaacgc
     3601 gatgaagaag cccagcatca acagatattc cacaccactg gacattggcg tattcagcag
     3661 ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag
     3721 gatggcgatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccgc
     3781 cgtgatacgc gttttaccat cagaggcttt atgcccccac agtgcgatca ggaagtacat
     3841 cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac
     3901 gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc
     3961 gatctctttc cacgaacaga gtaccgccag cacaccgagc agaccggtca gcacgaccat
     4021 cagcagcgac agcccgtcaa tggcgagatg gatagagata ccaaaacgcg ggatccacgg
     4081 catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc
     4141 ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca
     4201 gcgcggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc cgataaaggg
     4261 gattagtatt aaccagggta ataacatagc taagtatgtc ccttatattt actcctgctc
     4321 aggctatctc actcgccagc ccgaatcccg gcagtgctcg caatcctcac gtacttaagt
     4381 acgctccggt tgctgtgcgc tggcggtatc cggtctgact tcgccgatga cgcctgtact
     4441 tcaggaacag ttacctaaaa tttattctta gttcacagac ccggcgcaac gccgggtata
     4501 ttcactcagc caacaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     4561 atgctcatgg atgccacata ccagcgcaga taaccgttct cgcttaacag caggccttta
     4621 cctgcaaagc gggaaaggac agccgggatg ttcatcattg agttcagcgg atcgcgtttc
     4681 agcaaccagg caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag
     4741 ccccaggcgt tgtaccacca ggtgcccagc agacggcccg gcgcactgtt ggcgatggag
     4801 gtcaccagag tacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg
     4861 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4921 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4981 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     5041 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     5101 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     5161 tccttactga agaagcccgc agtgactagc ggcagtgccg acagtgctgc gccgcccacc
     5221 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     5281 tgttcgtgat ggcaggcgag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     5341 aacgcgtggg tcatcaagtg aaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     5401 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     5461 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc caacaatgcc caccagatgc
     5521 agaacttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     5581 gttaccatcg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     5641 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     5701 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     5761 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagcgc gaaagcgagg
     5821 aacacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5881 tcggtgtaat agaacccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5941 tacatcagca gcaggttgtc ggcaagcacc agaaccacca tgctggcgat aaacaggttg
     6001 gtgtaagcga agaagcgaga gtagccctct tcaccgcgca tataccagga ggcgtacatg
     6061 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     6121 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     6181 tatgcctgct cgccgttagc gaagaagtca acgccaataa aggcagttac cagcgccgcc
     6241 aggcccacag agccaacgcc gacgatcgcc gagacgtttt cagaccagcg cccacgggag
     6301 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     6361 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     6421 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     6481 cctgaccgtc ggtctggccc cagtaacttc cggcgaccac aaaggccagc gcggaagcgt
     6541 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     6601 ttaagccaag aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     6661 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     6721 acgctcttca cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc
     6781 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6841 tggcgtaccg tcgatacctt gatcgttaac accgagaatg gcgtaaacaa tcaccaccaa
     6901 catgatggct gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6961 ttgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     7021 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ctagtgagaa
     7081 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     7141 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     7201 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     7261 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     7321 tactccgggt atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc
     7381 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     7441 gcttcttcgc acaaaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     7501 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     7561 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     7621 atacgaccac gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg
     7681 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     7741 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7801 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7861 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7921 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7981 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     8041 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     8101 gctcggcttc cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa
     8161 aggtaataaa accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga
     8221 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     8281 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc accgagcagc gagtatttgt
     8341 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagaccggcc atcatcagga
     8401 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     8461 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     8521 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     8581 ggagcgaacc accccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     8641 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     8701 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     8761 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc
     8821 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagagtgac
     8881 cgtgttgcca tcgtaactaa aggagacgcg tgtacctgcg tttacaccca acttcgcggc
     8941 atccgctggg ttgagtttga tgtacggctg cggcatacga cactggaaga ccggagcacg
     9001 ctgtgacaat tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc
     9061 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     9121 aaacagacgc acacccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     9181 gttccacgcc tgcggggagt tccagcccgg agcccaggca aacggcactt gcgaacggtg
     9241 cgcggtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     9301 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     9361 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatctggcg cagcatcttt
     9421 gatacccgcc agttccggga ttttcgccac aacagcgtca attacatggt cgagctgcgt
     9481 ccagtccact tcacggctca gcaaggtgct gtgcagcgag tgcagccagc gccaactttc
     9541 cagcatgaca gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc
     9601 gcgaccttca ttattaatta ccgtaccgtc gctttcagca aagctggcag cggaaagcac
     9661 cagatgggcg ttttccatga tcgctgtgcg ttgatggtca accaccatca ccagcggcgc
     9721 tttagccagc gcagcattca cgcgggtagc agaagcgtga cgatgcagat cgttttccag
     9781 caccaccacc gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgatcc
     9841 accgcccata atgcccaacc ccatgctgtt gacggaacgg gcaatcatgg tgataccgac
     9901 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctaa
     9961 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacccgcca gtgcctgcac
    10021 gatgacgtcg attttgcttt gcagctcagg ttcaataccg tcaaccgctg gcgcagagtt
    10081 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
    10141 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
    10201 cttagcacgt tgaccgatat tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
    10261 tgccgccatt tcgcgcgctt tacctttcac cgcctggcgc actgccagcg cgacgcgcgc
    10321 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
    10381 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
    10441 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
    10501 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
    10561 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
    10621 acgacgctgt actggctgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg
    10681 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
    10741 cagttcgcca taacgctcgc cggggctgat gttacagccg atggaacatt gctggcagat
    10801 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10861 aaatacgccg gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc
    10921 gtcttccggg cgaccgaagt agacgttgtc atgcgcgcca taaacgccca gatctgtacc
    10981 gtcagcgtaa tctttgtagt aacgcacgca gcggtagcag gcgatgcagc ggttcatttc
    11041 gtgagagatg aacggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    11101 acggaagctg tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc
    11161 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    11221 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    11281 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    11341 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    11401 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    11461 tacatgaatt gtagccatta gcatgcttcc agttttctca gtcagagact gagcgttaat
    11521 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    11581 tattgctgaa cggctgtttg atccccgcct caaattcttc gcggaaatat ttgatggcgc
    11641 tctgtaacgg ctccacagca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    11701 atcgacacag ttgctcaagt gtttcgatat cgcccggctg accttcgcca cgctccagcg
    11761 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11821 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atttcatggt
    11881 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11941 ccatcggcag gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgccg cctggctgcc
    12001 aggctttgaa tttcagaccg tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    12061 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    12121 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    12181 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    12241 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    12301 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    12361 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaatccc gcttcagtcg
    12421 cttcggcaat ggcgcggcgc agatttactg ccgcttcaat atattcgcca cgcaggaaga
    12481 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    12541 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    12601 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaagc
    12661 cagtcgaaaa gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    12721 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12781 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12841 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12901 cggggtcaga ttcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12961 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    13021 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccattga tatgacagac
    13081 cacgctgtcg caataacgga tcacatggcg gccaaccggc tggcggaaga tctggctgta
    13141 gaacgttgcc acgccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    13201 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    13261 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctcg atcgcttcac gctctgccgc
    13321 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    13381 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    13441 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    13501 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccttt ggtcgcctca
    13561 atcatctgga aggattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    13621 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    13681 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    13741 ctctggcgca gctcttccac tttaagcatt acgcgggtgt agcagtcaga aacaccacca
    13801 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13861 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13921 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13981 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    14041 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    14101 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    14161 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatatacag caggtggctg
    14221 ttgatgcgga acagttcgga gagcattacg cgaatgacgt taacgcgagc cggcacggtg
    14281 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    14341 tattcgatac ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc
    14401 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    14461 agctgcaaaa cgatacggaa agcaccgtgc gccgacgggt ggttcggacc gaggttgagg
    14521 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    14581 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    14641 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    14701 cggcgcaggt tcgggtgacc gtcgaatgta atgccaaaca gatcccaggt ttcacgctca
    14761 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14821 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14881 gaaaaatccg cggcaggtaa cccttcgcgg tgcgtgcgca gacgttcgtc catgccgtgt
    14941 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    15001 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    15061 gcatccggcc caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg
    15121 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    15181 ccgtggtgac tgtcggcgca gcaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    15241 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    15301 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    15361 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    15421 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    15481 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    15541 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    15601 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    15661 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    15721 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15781 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15841 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15901 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15961 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    16021 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    16081 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    16141 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    16201 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    16261 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacatag gccaatggca
    16321 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    16381 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    16441 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    16501 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    16561 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca
    16621 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    16681 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    16741 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16801 cgtatttttt aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgtacgg
    16861 ggcgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac gatgtgaaga
    16921 aggttcttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta
    16981 cactattgtc tcaggaatta tctatcgtcc gtcgattact cgatatccct ttcaatcaac
    17041 aatgaatcat ccccttccgg agcagatatt gggctgtatt gccacggatt atggtagctc
    17101 tccatcgcct gatagatcac ctgggccagc tcattattac tggacgggtc atagcacagc
    17161 aaatattcgg tatcaggtag cggcggtaag ccatctactc cactcagcac acgcaagtcc
    17221 ggactcatca tctcgaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga
    17281 acggccggaa gcgtcgaggc gacataagcc agtcgccagg gaatatctgc tttattaagc
    17341 gtcgccagca ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc
    17401 tcaccttttt gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg
    17461 ttcaatgcct taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat
    17521 tccagcattt ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga
    17581 taaaccgaac tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa
    17641 gcaccgatag ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca
    17701 ttaaaacgca ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt
    17761 ttgttgcgac cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt
    17821 tgctgactta cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc
    17881 agatcggcaa cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga
    17941 tttgcactta tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg
    18001 cacacacaaa caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc
    18061 ggacgaaagt aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat
    18121 aatgcctgaa gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca
    18181 ctcttttcta tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca
    18241 ctctcgtgca gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaggcggca
    18301 tcaagagcag ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa
    18361 gggtcattgc gaattatctg atgcaaagtt atgttatgtt aggcaaagta atcttctat
//
LOCUS       DAZGXS010000093        18261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGXS010000093 DAZGXS010000000
VERSION     DAZGXS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            40..1179
                     /gene="arnB"
                     /locus_tag="ACHKG5_003281"
     CDS             40..1179
                     /gene="arnB"
                     /locus_tag="ACHKG5_003281"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0869325.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1183..2151
                     /gene="arnC"
                     /locus_tag="ACHKG5_003282"
     CDS             1183..2151
                     /gene="arnC"
                     /locus_tag="ACHKG5_003282"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0869326.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2151..4133
                     /gene="arnA"
                     /locus_tag="ACHKG5_003283"
     CDS             2151..4133
                     /gene="arnA"
                     /locus_tag="ACHKG5_003283"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP0869327.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4130..5020
                     /gene="arnD"
                     /locus_tag="ACHKG5_003284"
     CDS             4130..5020
                     /gene="arnD"
                     /locus_tag="ACHKG5_003284"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0869328.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5020..6672
                     /gene="arnT"
                     /locus_tag="ACHKG5_003285"
     CDS             5020..6672
                     /gene="arnT"
                     /locus_tag="ACHKG5_003285"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0869329.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6669..7004
                     /gene="arnE"
                     /locus_tag="ACHKG5_003286"
     CDS             6669..7004
                     /gene="arnE"
                     /locus_tag="ACHKG5_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0869330.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7004..7390
                     /gene="arnF"
                     /locus_tag="ACHKG5_003287"
     CDS             7004..7390
                     /gene="arnF"
                     /locus_tag="ACHKG5_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0869331.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7384..7650)
                     /gene="pmrD"
                     /locus_tag="ACHKG5_003288"
     CDS             complement(7384..7650)
                     /gene="pmrD"
                     /locus_tag="ACHKG5_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0869332.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7760..9115)
                     /gene="menE"
                     /locus_tag="ACHKG5_003289"
     CDS             complement(7760..9115)
                     /gene="menE"
                     /locus_tag="ACHKG5_003289"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0869333.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9112..10074)
                     /gene="menC"
                     /locus_tag="ACHKG5_003290"
     CDS             complement(9112..10074)
                     /gene="menC"
                     /locus_tag="ACHKG5_003290"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0869334.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10074..10931)
                     /gene="menB"
                     /locus_tag="ACHKG5_003291"
     CDS             complement(10074..10931)
                     /gene="menB"
                     /locus_tag="ACHKG5_003291"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0869335.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10946..11704)
                     /gene="menH"
                     /locus_tag="ACHKG5_003292"
     CDS             complement(10946..11704)
                     /gene="menH"
                     /locus_tag="ACHKG5_003292"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0869336.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11701..13371)
                     /gene="menD"
                     /locus_tag="ACHKG5_003293"
     CDS             complement(11701..13371)
                     /gene="menD"
                     /locus_tag="ACHKG5_003293"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0869337.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13460..14755)
                     /gene="menF"
                     /locus_tag="ACHKG5_003294"
     CDS             complement(13460..14755)
                     /gene="menF"
                     /locus_tag="ACHKG5_003294"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0869338.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14834..15139)
                     /gene="elaB"
                     /locus_tag="ACHKG5_003295"
     CDS             complement(14834..15139)
                     /gene="elaB"
                     /locus_tag="ACHKG5_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0869339.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15194..15655)
                     /locus_tag="ACHKG5_003296"
     CDS             complement(15194..15655)
                     /locus_tag="ACHKG5_003296"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0869340.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15720..16637
                     /gene="rbn"
                     /locus_tag="ACHKG5_003297"
     CDS             15720..16637
                     /gene="rbn"
                     /locus_tag="ACHKG5_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0869341.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16825..18048
                     /locus_tag="ACHKG5_003298"
     CDS             16825..18048
                     /locus_tag="ACHKG5_003298"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFP0869342.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEI
                     KNSHPKNRASSKRVS"
BASE COUNT         4412 a         4862 c         4546 g         4441 t
ORIGIN      
        1 atataagtgg acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt
       61 tcgagaccag caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt
      121 tggatcacaa ccggtccgaa gaatcacgcg cttgaacaag ccttttgcca gttgacggga
      181 aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg
      241 ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc
      301 aatatgattt ccttgcaggg tgcaacgccg gtaatggtgg atgtcgatcg cgatacgctg
      361 atggtcacgc ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg
      421 gtgcattatg ccggtgcgcc agcagatatt aacgccattc gcgccattgg cgaacgttac
      481 ggcatcgcag ttatcgaaga tgctgcccat gccgtaggta cgtattacaa agggcgacat
      541 attggcgcaa aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgcg
      601 gaaggaggcc tgattgttac tgataatgaa aatattgccc gccagctacg gatgctgaaa
      661 tttcacggcc tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct
      721 gaagtcttaa cgccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg
      781 acacagttag ccaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat
      841 cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt
      901 cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat
      961 gcgttgatgg aagcgttaaa agaaagaggt attggtaccg ggttacattt ccgcgccgct
     1021 cacacacaaa aatattatcg cgagcgtttt ccctcgctgt cgttaccgaa taccgaatgg
     1081 aatagcgaac gcatctgttc tttgccgctg ttcccggata tgactaccgc cgatgccgac
     1141 cgtgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct
     1201 gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta
     1261 atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt
     1321 gatgacggca gtagcgataa ttccgcgcat atactggtcg aagcctcaca agcggagaac
     1381 agccatattg tatctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg
     1441 ggattcagtc acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg
     1501 ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact
     1561 gtacgccaga accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg
     1621 cttattcagc gcaccaccgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat
     1681 cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt
     1741 ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcatcatgc cgagcgtgag
     1801 tatggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctggtgacc
     1861 tgccttacca caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga
     1921 ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg
     1981 gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc
     2041 ggcatgggat tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc
     2101 cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg
     2161 tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg gctgccggtt
     2221 acgaaattag cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt
     2281 cggtggctcg tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc
     2341 atccgctgtg ggtggaacgc attgcccaac tctcaccaga ggtgattttc tctttttatt
     2401 atcgccatct tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc
     2461 acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg
     2521 gtgaaacaga aactggcgtt acattgcacc gaatggtaaa acgtgccgat gccggggcca
     2581 ttgtggcgca actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata
     2641 aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta
     2701 atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg
     2761 atgacagttt cctcgaatgg cataagccgg catccgtact gcacaacatg gtacgtgccg
     2821 ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt
     2881 cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc
     2941 cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt caccggacaa gcgggcgacg
     3001 gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct
     3061 tgaatagcca gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga
     3121 atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt
     3181 acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact
     3241 ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg
     3301 atgtcgtctt gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc
     3361 gcgtatttga actcgatttt gaagagaatc tgcgcattat ccgctactgc gtgaagtacc
     3421 gtaagcgaat catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact
     3481 tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt
     3541 cggtatcaaa acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac
     3601 agttcaccct cttccgcccg tttaactgga tgggaccacg actggataac cttaatgcgg
     3661 cacgaatcgg cagctcccgc gcgattacgc aactcattct caatctggta gaaggttcac
     3721 cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt cgcgatggta
     3781 tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca
     3841 acattggcaa tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga
     3901 gcttcgaaaa acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg
     3961 aaagtagcag ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc
     4021 gcaatgccca ccactgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg
     4081 aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt
     4141 aggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct
     4201 ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa
     4261 tatgggccgc catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc
     4321 aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa
     4381 agagattggt catgccaatg ccgatatcat tcgtgaagcg gctaaatatc acgaagtcgg
     4441 cctgcacgcc tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac
     4501 aatgatcgac gatattgcac gcggccttcg cactctggaa gagattatcg gtcaaccggt
     4561 aacctgttct gccgctgcgg gctggcgtgc cgaccagcag gtgatcgaag caaaagaagc
     4621 gttccatttg cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga
     4681 atcaggaaac cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat
     4741 tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga
     4801 taaaggcacg ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa
     4861 ttttgtggat ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga
     4921 actgttatca gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga
     4981 aggctggctg ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt
     5041 atcggcctct tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgcta
     5101 tggcaacccg atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac
     5161 tggattgtgc cccatctgtt agggctacgt tattttgaaa aacccattgc cggatactgg
     5221 attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt
     5281 atctttgcga ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc
     5341 gataaacgtc tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc
     5401 atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc
     5461 agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg
     5521 ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg
     5581 gtattaagcg tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac
     5641 ggctggctgg cggtcatcag ttgcgtactg acggttctcc cctggggact gacgatagcg
     5701 cagcgggagc ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca
     5761 ctggatgatg cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt
     5821 agtctgccgt ggctgggatt actccccggt gcactataca caggctggaa aaaccgcaag
     5881 cattccgcaa ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtt
     5941 gctaagggta aattgcccac ctatattctt tcctgctttg cacctttggc aatgctgatg
     6001 gcgcattacg ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg
     6061 attaacatcg cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga
     6121 ccaatgaaca cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg
     6181 attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg
     6241 tcttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac
     6301 agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag
     6361 ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagtctg
     6421 caacgcgatg acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat
     6481 ccggatgcga aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat
     6541 cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt
     6601 ctcgccattc cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat
     6661 cgtcccaaat gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt
     6721 tgtgtcagaa acaggcaacc tgctttgtgg cgataagcaa acggcgcaaa catatcgtgc
     6781 tgtggctggg actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct
     6841 tgcagaacgt accggtaggt attgcctacc cgatgttaag tctgaatttt gtctgggtga
     6901 cgctggctgc agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg
     6961 cgttcattat tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg
     7021 attattcagc gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcgagtca
     7081 tctgccgccg atgacgcact tatgggattt tatcgcggcg ctgttagctt ttgggctgga
     7141 tgcccggata ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa
     7201 aacactacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct
     7261 ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact
     7321 actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca
     7381 acgttactga gttttccctg ccactttaca ctgccgttcc cactcatcgg ggctataaca
     7441 gcttgcagag aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt
     7501 ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat
     7561 catttttatc gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt
     7621 gcagcacgat tttttgacca gccattccat tgtattatcc tgtttgctaa gagttttcat
     7681 atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg cagaactgag
     7741 aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg
     7801 aaattttaat accgccgttt ttcagctccg gcggcaaagt cagccaacgt accggttgct
     7861 gaaaacgggc cagcttatct ttcacccatt cactaagatc aacgctctcg tggtcatact
     7921 ccatcaccgc caccggtcga tgaccaaact ccttgtcggc aacggggacg ataaacacct
     7981 gcaaaaccgc aggatgtgca gcaattacgc gctcgacttc ttccgtctga ataccctctc
     8041 cgccactgaa gaatagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
     8101 caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt
     8161 tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt
     8221 tcacttcccg acccggcagc ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac
     8281 acaccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc
     8341 gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg
     8401 aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag
     8461 tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag
     8521 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
     8581 accagtcatc gtgatcgcca aacggaatca gcgataacac cccttcggca ctggcaagat
     8641 gggcctgata agtatggaca gcggctttcg gcaacccggt agaacccgag gtcaacgtca
     8701 ttgagcacag acgcgtcggc tgccacgtag cgacatgtgc gccttcaacc cgctgaatgt
     8761 gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg
     8821 tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca
     8881 cccgcgcccc gcattgcagt aacgccagcc aagcgagcag cgtttgcggc gtattccacg
     8941 cccgcaacat cacgccgctg tcctcaacca caccctgcac cgcaaatccg gaggctaatt
     9001 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
     9061 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa
     9121 ccgctccagt gcatcaactt ccacgacagg cagcgtgcta cccggccagc gacgtacctg
     9181 ctgcgcctgc attagatcca gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc
     9241 agcaatccgc gccagttgcg ttaagcctaa gctcgattca atggaagaac tgatcactgc
     9301 cgtcagcccc agcgcgtgcg ccgcctgtac ctgctcgcga actttctcca gactgcccgt
     9361 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg
     9421 ctcgcgcaag ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga
     9481 atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatccgggtt
     9541 aacgtatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg
     9601 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc
     9661 ttcgtacaat cccactttga ccttcgccac tttctcgcct ggcatatctg caagtttgag
     9721 gatcagatcg tccggatcgc cattacacag tggtgccgta cggtaattgg ccgcctgcgg
     9781 gagcgtatct gccagctccg ccaatgcaca acttacgcca aaggcaacgg aaggcatctg
     9841 cggtaattcg caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc
     9901 ctcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg
     9961 ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    10021 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    10081 ttccgtttga atttgctgaa gtcaggctga cgtttctggt tgaaggcgtt gcgaccttcc
    10141 tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagacct
    10201 gcctgaccgt cacagtcggc gttcagtgca gctttcaggc agcgcagcgc catcggactg
    10261 ttttgcagca tttcacggca ccagcgaacg gtttcttttt ccagatccgc cagcggtacc
    10321 acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac
    10381 cagatttcac gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg
    10441 ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgccgcgata
    10501 gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggaatagcc agccaccatc
    10561 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    10621 tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg
    10681 gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgacgtt atcgtcataa
    10741 cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    10801 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    10861 tcctcaaagc cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca
    10921 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    10981 cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    11041 cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    11101 acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg
    11161 cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcacc accagctccc
    11221 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg
    11281 cggttaatgg ttcggaacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    11341 cgcgttgttc agcattttgc agccccggat gaccgccttc gacaacaacc ccacaaagcc
    11401 ccgccagccc ctggcaagcg gccatcatcg ccacccgtcc gccaagcgag taccccacca
    11461 gccagaagct aaggatgttg taactaacca gggttttacg cagtaagtcg gtgacatcat
    11521 caaatccatc gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc
    11581 gtgagtagtc ggcaaacgcc tcgccgactt cctgccattc gaggcaatcg ccggaaaaac
    11641 cgtgcaaaaa caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga
    11701 tcataaatgg cttacctgcg ccagcagctg ctggagcgtt tgcgcgccgt cggtgtcgtt
    11761 aaccaccatt tcaattaccg tggtggtcgg agtacgccag gcgtcggcaa gtaccgtttc
    11821 aagttcctgc cagttttgcg gacgatgata tttcagctca aacatcgcgg cagcgtgctc
    11881 aaaatggacg ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg gcgttggcag
    11941 cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac
    12001 ctggcgcaat aacgccagcg cattcaggtc gtaaagtgcg gagagatcgc ccacaatcgc
    12061 cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat
    12121 accgctggcc ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    12181 aatcagacgt accaccaggc tgttaccgac aaacaattgc ccctgttcag gcagatagtc
    12241 gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacaggcaa taaccgcctg
    12301 cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    12361 atgcagttcc agccagtcgg caatattggc aattaagcgg cgtccacgat ggtgtgccgg
    12421 atcaagccgc ccttcgatgt catccacaat ccagtactct tcgggttcac agcttgcctg
    12481 ccattgcagg agccgtttgc ccgtcaggct gcttcccagt tgcaccacaa tttgcgcctg
    12541 ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg
    12601 tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagggc
    12661 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgccg
    12721 ccagaagaac cagtcgcgct gtttttcgct ctcaagacga ggcgcttcac gcagccacgg
    12781 cttatcatcc tgccaccagt cacccagacg ctgttgccag ctaagcccag tgtcgtccat
    12841 ctcgccatac agcggttcag caaacgggca gttgatatgg accccaccag catgaagcgt
    12901 accgagagca tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    12961 cggcagtgaa atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg
    13021 attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaacagga tcagtttttc
    13081 tccggttaac ccagcttcaa tcagtgccgg atagagattt gctaccgccg tgccggaggt
    13141 cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag
    13201 tccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    13261 taacggtgta gaacgcgagc caggggcgat gcagatgtgt cttacgccgt gacgcgttaa
    13321 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    13381 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    13441 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    13501 tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat
    13561 atagccgcac gacattgccg ctgatttttg ctgagcgcag ggaaacgcag aattcgcttt
    13621 gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgat
    13681 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    13741 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    13801 gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat
    13861 cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat
    13921 catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta
    13981 ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    14041 gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattga
    14101 gattcagtcg acgactggca gccatcatcg ccgccgcatt gaccggactt gcgaaatgca
    14161 ggtcagttgc ccgagcaagc accactttgt cgagctcacc ttcggcgatg gttttcgttg
    14221 ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtagtgg
    14281 ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tctcttgcct
    14341 taatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac
    14401 cgccacagcg tcgccattcc aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg
    14461 cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    14521 cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat
    14581 tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat
    14641 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    14701 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    14761 cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg
    14821 gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcac
    14881 ccacaccaat tccctgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct
    14941 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    15001 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    15061 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat
    15121 caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga
    15181 caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat
    15241 cttcttcata gatctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat
    15301 gcgcctgcgc cccgaggtag acaggcttat caggccagtg atgcgtgcaa gtgtcgaggg
    15361 ttttactcat cagttgctgc cccacttttt cgccacgcaa cgcttcactg acaatcaccc
    15421 gaccaataac gaccggctca agatcatcat cacttttcag aatcctcgca tatgccacca
    15481 gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga
    15541 tatcctgata agggcaattt tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg
    15601 cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc
    15661 tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca
    15721 tggaattaat ttttttaggt acttcggccg gtgtgccaac ccgcacgcgc aatgtcacgg
    15781 caatattgct aaacctgcaa catccgaccc agagcggact ttggttgttt gactgcggtg
    15841 aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt
    15901 ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt
    15961 ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg
    16021 tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa
    16081 ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt gaccgcttat ccaatggaac
    16141 acccactgga atgctatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg
    16201 cccaggcatt aaaagctgct ggcgtaccgc ctagcccact gtttcaggaa ttaaaagcgg
    16261 gcaaaacaat catgctggaa gatggaaggc agattaacgg cgcagattac ttagctgctc
    16321 cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg
    16381 acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag
    16441 ccaaagccaa tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg
    16501 ctggagtcgg taagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc
    16561 agcacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactagcg aatgatttca
    16621 ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc
    16681 gcaacccgcg ctacatatat ccactcacac gggcatattg agcgattttt gatatttatt
    16741 catctcctta cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac
    16801 aacctttgca actaaaagga gcatatgatg gttacagttg tcagcaatta ttgtcaatta
    16861 tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg
    16921 cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gcattgagtt actgcataat
    16981 attgcgttag gttatgatga atttgggaaa aaagctgaag atattcttta ccatattgtt
    17041 agaaacccaa caaatgatac cctatcgatt atcaagctta taaaaaatgc ctgtttaaaa
    17101 ttatataatt tggcacatac cgcaaccaaa caccccctca aatcacatga ttcagataat
    17161 ctcctgttca aaaaactatt ctccccttcg aaattaatgg caattatcgg tgaggacatt
    17221 cctctcatat cagaaaaaca gtcactttca aaggtacttt taaatgataa gaataatgaa
    17281 ctgagcgatg ggacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagct
    17341 tgctatctta aaaagatcgc cgccaatgca aaaaatactc aagtcaatta tcctactgat
    17401 ttctacctcc ccaattccaa cagcacttac ctggaagtcg ctctcaatga taatattaag
    17461 agcgatccat catggccgaa agaagtccag ttattcccca taaatactgg cggacactgg
    17521 atattagttt cgctacagaa aatagttaat gaaaaaaaca acacacaaca aataaaatgc
    17581 atcatattca attcattacg tgcactaggc catgaaaaag aaaattcact taagcgtatc
    17641 attaacagtt tcaatagttt caattgtgac cccacgagag aaacgccgaa taataagaat
    17701 ataacagatc atttaactga accagagata atatttttac atgccgatct tcagcaatac
    17761 ttaagccaaa gttgcggtgc atttgtgtgc atggcagccc aggaagtgat tgaacaaatg
    17821 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
    17881 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgtctgga aaacagaaat
    17941 tgttatttag ataaatatgg cgatgcaaat atcaatcatt attatagaaa cttagaaata
    18001 aaaaactcac accccaaaaa tagagcatca agcaaaagag taagttaaca tcattgttaa
    18061 ttaattcgac cagtcagcaa atatgctgac tggtcgaaca tagtttgcta aaaagtcatc
    18121 actgactgat gtcagtcaca ccacccgcca gttcaatctt cgggtatttc cagcgtatct
    18181 cggcacgcga ttgctggtct gttgatgtag tggattgttg ttgctgactt ttcttaccct
    18241 cagatttctg ccccctgtca a
//
LOCUS       DAZGXS010000094        18058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGXS010000094 DAZGXS010000000
VERSION     DAZGXS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            47..841
                     /gene="hyxR"
                     /locus_tag="ACHKG5_003299"
                     /pseudo
     CDS             47..841
                     /gene="hyxR"
                     /locus_tag="ACHKG5_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            995..1756
                     /gene="hyxA"
                     /locus_tag="ACHKG5_003300"
     CDS             995..1756
                     /gene="hyxA"
                     /locus_tag="ACHKG5_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP0869343.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTSSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1798..2880
                     /locus_tag="ACHKG5_003301"
                     /pseudo
     CDS             1798..2880
                     /locus_tag="ACHKG5_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2870..3058
                     /locus_tag="ACHKG5_003302"
     CDS             2870..3058
                     /locus_tag="ACHKG5_003302"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0869344.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            3240..3908
                     /gene="ykgH"
                     /locus_tag="ACHKG5_003303"
                     /pseudo
     CDS             3240..3908
                     /gene="ykgH"
                     /locus_tag="ACHKG5_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(4151..4846)
                     /gene="ykgG"
                     /locus_tag="ACHKG5_003304"
     CDS             complement(4151..4846)
                     /gene="ykgG"
                     /locus_tag="ACHKG5_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0869345.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(4839..6266)
                     /gene="ykgF"
                     /locus_tag="ACHKG5_003305"
     CDS             complement(4839..6266)
                     /gene="ykgF"
                     /locus_tag="ACHKG5_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0869346.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(6277..6996)
                     /gene="ykgE"
                     /locus_tag="ACHKG5_003306"
     CDS             complement(6277..6996)
                     /gene="ykgE"
                     /locus_tag="ACHKG5_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0869347.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(7523..8377)
                     /gene="rclR"
                     /locus_tag="ACHKG5_003307"
     CDS             complement(7523..8377)
                     /gene="rclR"
                     /locus_tag="ACHKG5_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0869348.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            8603..9928
                     /gene="rclA"
                     /locus_tag="ACHKG5_003308"
     CDS             8603..9928
                     /gene="rclA"
                     /locus_tag="ACHKG5_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0869349.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            10037..10273
                     /gene="rclB"
                     /locus_tag="ACHKG5_003309"
     CDS             10037..10273
                     /gene="rclB"
                     /locus_tag="ACHKG5_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP0869350.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            10285..10878
                     /gene="rclC"
                     /locus_tag="ACHKG5_003310"
     CDS             10285..10878
                     /gene="rclC"
                     /locus_tag="ACHKG5_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0869351.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(11038..11907)
                     /locus_tag="ACHKG5_003311"
     CDS             complement(11038..11907)
                     /locus_tag="ACHKG5_003311"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0869352.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVYKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            12156..13013
                     /locus_tag="ACHKG5_003312"
     CDS             12156..13013
                     /locus_tag="ACHKG5_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0869353.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(13134..17387)
                     /gene="fdeC"
                     /locus_tag="ACHKG5_003313"
     CDS             complement(13134..17387)
                     /gene="fdeC"
                     /locus_tag="ACHKG5_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP0869354.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            17953..>18058
                     /locus_tag="ACHKG5_003314"
     CDS             17953..>18058
                     /locus_tag="ACHKG5_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0869355.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDA"
BASE COUNT         4776 a         4370 c         4040 g         4872 t
ORIGIN      
        1 tgaatcaaaa tgtcaggttt tgtagctatg gcgtggacga gtctgaatga agaagcctct
       61 ggttattatc agtgcctgtc agtttaccag gctggcactg gagtctctca tcccagcgga
      121 caggtatatc gtgcgggtgt acagcaacgt cactacagag gttgaattcg ttttaaagac
      181 ctcctgtggc tatctgttgg ctgatatccc ttagcttcct tcgggggagt tagttcttct
      241 ggcttgtctt gtccgcttgc ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga
      301 ctccgactgg ttcgataatg ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg
      361 caccatgctg acagcgagtc gttatcagaa tcaaattatc cgctggttac tacgtccttt
      421 gcccggcgat atgagcgatt tctggctcct gactgcacgg gagttgagcg tgcttaagat
      481 tctgatgcag ggcatgagtt tcagtgagat tgcccgttat gaacaacgca gttcaaagac
      541 cctgcatgcg attgccacac gggcattaat gaaacttgga ctgggaactc tgagcgattt
      601 caggctgctc tatacgggat gcggtgacag cagagtgaaa cggaatatac ggctacatgc
      661 aagatatgtc ggacaacatt ccgctcatcg actcactcag tatgtgcaga acatggtatc
      721 cggaattctt tatccagaga aagttgccgt tggtgaatca ataaattctg acgccatttc
      781 cggaaaagta cttcccggac aaagaaaaaa tatttctaaa aaaagaccag gattgttatg
      841 acacaacagc gttgttacta ccccccactg cggcgcgtac ggactcgcta cagtgtaggg
      901 gccaaaatat ttctactgct ctgggctgca ggattgggag gcacgctgta cagcctgtac
      961 agcgtgctgg cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt tctgtccctc
     1021 aggaattgta ccagcgcagt tcgcagctct atcaaccagt tctgtcggca atgttacctg
     1081 catcggaaca gaagaggagt tacgcaataa attgcggcac aggccacaga gtgtcgtgat
     1141 atcggcaggg cgacctgccg aatgcgctga aatgtggttc cgattttatc gggatcactc
     1201 attcgtggtg gtgttgtgcg tcgcgccatt ttttctgcca ccggatgtga gtatttctgg
     1261 cgttctcaaa aacctgaggt tactgaaacc aggaatgtca gtagagcatg tcatcagtat
     1321 tgcaaattcc tctggtgggt tatcagggct gaaacatgca gaaaatttgc cagttatgga
     1381 cagttactct gtctttatga aagaggtgaa taaccgcaca aagacaatcg tcatgtcaga
     1441 acgcttcccg gaaaagcaaa agaaggtgtt gtcgttgctt cttgccggac acagctggga
     1501 atacagtgcg cagtttctca aaacggggat aagacagata tggcttgccg agcagtcgct
     1561 aaaaaagcgt tggggaatac cggacagcat gtcattgcgg gaagccctgc tgctgagcag
     1621 caataaattt cacggtgatg gaaatgcatt ggaaaaaacg aatgccatga cattgcgtga
     1681 aaacggaaat accaacaggt attccgtggt ggtaaatgca gggacgcagg ttttatctct
     1741 gcataaatat aaataagtgg gaaattgaaa ttagttacca gaatggagaa tttcttcatg
     1801 aaaaacagta aggcatttta ccgcagcgca ttagcgacag ctattgttat ggctctttct
     1861 gcaccagcat tcgctgctga taacgcggta tcaactgatt cggttacgct gaataaagat
     1921 aagacgactc tggatcaaga tgttgttatt agcaatacag cagacaaaca gattacagcc
     1981 gtaacaatta atgcggcaga tgaagacctt aatgttgctt ttgctggtca tgatattact
     2041 gctgaatcaa cggcagacaa aaaattcgtt gaaggtgtaa aagttagtgg taacaaaaat
     2101 gttgtgatta atgctacaga ctccaccatc acagctcaag gtgaaggcac ctatgtccgg
     2161 actgcaatgg tcattgattc aactggcgat gttgttgtta atggcggtaa tttcgttgca
     2221 aaaaatgaaa aaggtagtgc gacagggata tctctggaag gggccaaggg aaataatgtc
     2281 acgcttaatg gcacaaccat aaatgctcaa ggtaataaga gttccagcaa cgcctccacg
     2341 gcaatttttg ctcaaaaggg tagcttattg aatagttttg aaggtgatgc aaccgacaac
     2401 attacccttg ctggctcaaa tattattaat ggtcggattg aaacaatagt tactgccggg
     2461 aataagacgg gaattcatac agtcaacctg aatattaagg atggctcagt aattggggcg
     2521 gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcacagg ttcagcaacg
     2581 caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct
     2641 gaaagcgaga attcagccgc taccacaaca aatgtaaaca tgaacgttgc ccgctcttac
     2701 tgggaaggta atgcttatac tttcaatagc ggcgataaag cgggtagtaa tctggatata
     2761 aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaaa tgccagtgta
     2821 tctctgcaaa acgagtctgt ctggaatgtt acgggatcct caactgttga tgctctggct
     2881 ccgtctgggt gatgagtctg tcaatgccag cattgacggt gcagcattcc gcgtgggtgc
     2941 aggtgtacag gctgatatca ccaaaaacat gggagcatat gcaagccttg actacaccaa
     3001 aggtgacgac attgagaatc cgctacaggg tgtagttggt atcaatgtga cctggtaaga
     3061 ataaagcagt cggttcccct gtcgactgtt ataccccctg caggcagcag tatgctgcct
     3121 gcaggctata aatatccagt attcatgatg ctggcttttt gctgatattt aacccgaaca
     3181 ttcctgatat agactattaa ttcatttatc tgcggcagat tttattttct ggatatatta
     3241 tgagtgaaca aatcaaacag gatatcgatc tgattgagat tttattttat ctgaagaaaa
     3301 agattcttgt tatccttttt attatagcta tctgtatggc aatggtgctt ttatttctgt
     3361 atatcaataa agataatata aaagtgagtt acagcctaaa aataaatcag acaacacccg
     3421 gcatacttgt taactgtgat agcaataata actttgcctg tcagacaaca atgactgaag
     3481 atgttattca gcgaattact acattttttc acaccagccc agatgtcaag aacagagaaa
     3541 taaagctgga atggtcagga gataagagag atttaccaac tgctgaagcg gaaatatctc
     3601 gcgtgcaggc gtctattatc aaatggtatg cgtcagaata ccataatggc aggtaagttc
     3661 tcgatgagat acaaacgcct tcagcaatta acagtgagct ttatacaaaa atgatatacc
     3721 tgaccaggaa ctggtcattg tatccgaacg gtgatggctg tgtaactata agctcaccag
     3781 aaataaaaaa taaataccct gctgccattt gcctggctct gggatttttt ctaagcattg
     3841 taatttctgt aatgttttgt cttgtcaaaa aaatggtaga tgaataccaa caaaactctg
     3901 ggcagtaata aacagcattc tgatagagag tgcctgtata aagctgaagt gacaatgacc
     3961 gggaagggaa cccgttttca ggaatggatg cctgtctttt tcactgtaaa tatcctggca
     4021 ttagtaatgc caggatattt acagaagagg agcataacca tgacggaacg gggttaagtc
     4081 aggttgtcct taacgctatt ttcaacctcg tatgccttct tcaggtttat gtccagactt
     4141 catacctctc tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc
     4201 cgacgacttt gataagctca atgtccgccg ttgagctggg accactgatg atgttaatac
     4261 atgacggcat gcgttcaccg gcctgcgctt tctgatgcaa tctttctgcg agttgcgcta
     4321 cacgcggcag gatagtgctt ttacgcagga taaaaagaga tgattccggg agcaggctca
     4381 atgaacgccc acgctcggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat
     4441 attcagcata cacgacaccc actttagcct gctctgcctg cgagatattc ttggcacctt
     4501 tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta atccctaatt
     4561 cctccagcct cgtgtcaccg ctaatcacga ccgactgatc tcccagctct ttacacagac
     4621 gtattgcagc ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc aacataacat
     4681 cgctggcaaa ctgaataaac gcgtcacagc gctgctgttg gttaagttgg gtaagccgct
     4741 cgttagcata gttattaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac
     4801 ccagtgcctg agcaacgtta ttcaaaaatt cgcctcgatt atccattctt tttctcctgc
     4861 gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt
     4921 gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac
     4981 cagcttgccg catgagcacc ggccatcatc ccgactttcc acaatcctgg atgactattg
     5041 gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc
     5101 atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgttg
     5161 tcacaagctg tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca
     5221 agtagcggag aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg
     5281 ccaatatggc gatatgccgg acaagtattc atacaagccc cgcagcgaat acagcgcagc
     5341 acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga
     5401 aactcttcag gaccatcaac gtgccccgct tcgcgaggtc ctgtcagcca ggtgttgtat
     5461 cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg
     5521 gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg
     5581 gtacacattc gcgcattacc ttcattggtc accaggcata ccgaacctgt ctctgccacc
     5641 gcgaaattac agcccgtaat acctatttca gcactgagga aatcttcgcg gattttttgc
     5701 cggatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt
     5761 agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagagggc
     5821 ggatcttgat ccagctggag aatatattca cccaaatcgg tttcaatcac ctgaatgcca
     5881 gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc
     5941 accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg
     6001 gtttttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga
     6061 taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc
     6121 tcccagagcc ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg
     6181 tttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa
     6241 tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca acacttcagc
     6301 aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga tgttcagcag
     6361 gcaactcacg tcagcaccaa ttaaatactc agggcggact tccatcaggt gcgcaacctt
     6421 ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc
     6481 gcagcaggta tcctgttcag caaaggtcaa cagctccagt ccacgcacat ttttcagcag
     6541 cgtaagtggc tcgtccttca cccccagctt acgggccagg ctacaagatg ggtgatacac
     6601 cgctctccct tgcaaactgg cacctacatc gactacccct aatttattaa caataaaaga
     6661 ggtgagatcc tgcatacgcg cggcaacctt ttcggctcgt gatgcccatt caggttcatc
     6721 cgccagatac gtcgggtaac tttttacggc ataggtgcaa gagccagccg gtgaaataat
     6781 gggatcgtcg ttatcctcca gtgcggcgat cagatttttc atccctggaa tcgcttcttt
     6841 gatataacca ctattgatcg caggctgacc gcagcatccc tgtttctccg ggaaatttac
     6901 gcgacagccg agtttttcca gtagcagcac ggagtctcgt gccattcttg atttcagggc
     6961 gtcaccaata caggtgacaa agaaattgac attcacaact aatactccat tacttcatgc
     7021 ccatttattc ccgatgtttc cccccacccg gggatcaatc atcaataaat atggcgttct
     7081 tttttaattg gctagcaaca taccacccct tatttgtatg acaaatgata gtctttcatc
     7141 caaacttcaa atgcaataac taattttatt tctttcggca tcgctcacat tttatgggga
     7201 aatcgacgat catttttggc ataagaaatc catttaaaaa tggctacatc ccttatacct
     7261 attcagagct acttattcag gggttattga gatatacact gatggtatac aaaaaaatta
     7321 ccgacaataa tcacgtatca agtaatagtc gggattttat ttaattgaag caatttagtt
     7381 tgcattttta tttaaaaact gagctagagt ccatcagaaa aaaatatata aagcccaaac
     7441 caccgttgga aatagaaata gttgaaaggc tcacaactcc cggaatgcgg attatctttg
     7501 ccctgatttc tggcggtttt attcagggtg caagctgtct gaccctttcc cgatattctc
     7561 ccggagtaca accaaactcg cggacaaacg ccttgtgaaa agatgattca ctggcatagc
     7621 ctactgactc agcgatcacc acaacaggga gcatttcccg ggaaaacatc tgggccgcta
     7681 tttgtagacg caactttgtt aatacagcca gcggcgtggt tccggaaaca tcacggaaaa
     7741 gctgggcaaa acttgcccgg gacatgtggg cgatgctggc cagcgattcg acgctccagg
     7801 cgtgtcctgg catttccagc atttgctgta ttaccgcacc aagacgtgga tgcagaagca
     7861 aactgagaat gtttttctca gtattaacct gtgcaatcca ctcacgcacc gcgagggtaa
     7921 agaatgtagc gcagatctgg ctacacagtg catccactcc cggcattgcc gatctggatt
     7981 cctgttgtaa aaacgggatc gcctccctca gccagttata ttccacgctg tggttaaccg
     8041 gtgctaaaaa cagcgtttcc ggcaaagacg ttaaaaaata acgtgccgaa tgttgcagcc
     8101 gaagagtacc acagacaata caggtcgatt cattatcgac atgactcagg cgatgagctg
     8161 aattttgtgg tagcaggacc acatttcccg ggcgtaatgt aaaaatctct cccgtcggca
     8221 tttccagctt cgccgctcct tgcgttaacg catgccaacg aataactgat aattccccgg
     8281 caccatgtgg aagctgccag tcacttccta acacgcaatt cttatcgatc gttccttgtg
     8341 gagcgttaag catcaacaaa cggctaaggg catccatatc agactcctga gtggtttttg
     8401 attacaaaca ccaacaatct tagtaaacaa ctaatctaat accatcaaat agttaatcat
     8461 gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa gacaaaatat
     8521 caagcgttgt gaagaaatgt tatttgctct tttgcgtcta tagtcatgat gtcaaatgaa
     8581 cgcgtttcga caggaaatca tcatgaataa atatcaggca gtgattattg gttttggcaa
     8641 ggctggaaaa acattagccg tcacgctggc aaaagcaggt tggcgagtgg ctctcatcga
     8701 acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgcatcc caaccaaaac
     8761 attggttcat gacgcacagc agcacacaga ttttgtccga gccatacagc gtaaaaatga
     8821 agtggttaat tttttacgta ataagaattt tcataatctt gcagatatgc ccaatatcga
     8881 cgtgatcaac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc atcggcctga
     8941 gggaaatctg gaaattcatg gcgagaaaat ttttattaat accggtgcac aaaccgtggt
     9001 tccgccaatt cctggaatta ccaccacgcc aggagtatat gacagcaccg gattacttaa
     9061 tctaaaagaa ttgcctgggc atttaggtat tttgggcggc ggatatattg gcgttgagtt
     9121 cgcctctatg ttcgctaatt ttggcagcaa ggtaaccatt ttagaagcag cttcgctgtt
     9181 tttgcctcgg gaagaacggg atattgctga taatatcgcg acgattttac gcgatcaggg
     9241 cgtcgatatt atcctcaatg cccatgtgga gcgaatcagt caccatgaaa atcaagtgca
     9301 agtgcatagc gagcacgccc aactggcggt ggatgcactg ttaatagctt ccggtcgtca
     9361 accggctacc gcttcgttac atccagaaaa tgccggtatc gcagtaaacg agcgcggggc
     9421 aattgtcgtt gacaagcgat tacataccac cgcagacaat atttgggcga tgggagatgt
     9481 taccggcggg ctgcaattta cttacatatc actggatgat taccgcattg tacgtgatga
     9541 gttactgggt gaaggcaaac gtagtactga tgatcggaaa aatgtgcctt attccgtatt
     9601 tatgacaccg cccctgtcca gggttggtat gacagaagaa caagccagag agagtggtgc
     9661 tgatattcag gtggtgacat tgcctgtagc tgcaattccg cgcgccagag tgatgaacga
     9721 tacccgtggg gtattaaaag cgattgttga taataaaacc caacgtatat taggggcatc
     9781 actgctgtgt gttgactccc acgagatgat taatatagtg aaaatggtga tggatgccgg
     9841 gctgccttat agcatattac gcgatcagat atttactcat ccgtcgatga gcgaatcact
     9901 caatgatcta ttttcattag tcaaataaac tcaaaatcag acgccagaac aaatattctg
     9961 gcgtctcaga gaaaagaatc ttattaattc cctgttactc tataacttct taatcacttc
    10021 attgatggta ttttatatgt ttaaaaaatc cgttttattt gcaacactat tatctggcgt
    10081 tatggcattt tccaccaatg cagatgataa aataattctg aaacatatca gcgtctcgtc
    10141 agtatcagca tcaccgacag ttctggagga tgccattgct gatatagcca gaaaatataa
    10201 tgcttcatcc tggaaagtca catcgatgcg aattgataat aattcaaccg caacagcagt
    10261 attgtataaa taaggatatt cacaatggaa aaatacctgc acctgttaag tcggggagat
    10321 aaaattggcc tggcattgat tcgtctgagt attgcgattg tttttatgtg gattgggtta
    10381 ttaaagtttg tcccttacga ggcagacagc attacaccat tcgtcgcaaa cagtccacta
    10441 atgtcgttct tttatgaaca cccggaagac tataaacagt atctgactca cgaaggcgaa
    10501 tacaaaccag aagcaagggc atggcaatcg gccaataata cctatggttt ttccaacggt
    10561 cttggcgtcg tggaggtgat tattgctctg ctggttttgg ctaatcctgt caatcgctgg
    10621 ttaggtttat tgggagggct gatggcattt accacaccgc tggtaacact ctcattttta
    10681 atcaccaccc cggaggcatg ggtacccgca ttgggtgacg ctcatcatgg tttcccttat
    10741 ttatccggtg ctggtcgtct ggtattgaaa gatactctga tgctggcagg tgctgtcatg
    10801 ataatggcag attcggcgcg ggaaattctt aaacaaggca gtaatgaatc cagttcaacg
    10861 ttaaaaactg aatattgata atccgaacac tgtctgttgt tcactttttc aggcgtcagg
    10921 ccccctttct gatgatgaag cctgacgcca tattaccgga gatatgacag agtgaagatc
    10981 aatatcactc ccctgttaaa agattcttgt cgtctcaggt aaaatacatg attaatatca
    11041 atcatgtatt tttacagcaa gacaaccacg aacaaattca ggattaaaat gttttaccga
    11101 ctccccaaca taacccaaat caagcgaact aatttgtgcc atttctttct ctgtcagtga
    11161 gaaatcccag atagcaaaat tttcttcgat acgctgttga tgtgtcgatt tcggaataac
    11221 gaccactccc cgttgaatat tccaacgcag aatgacttga gaaatcgatt tttgatgggc
    11281 atctgcaata ctctgaagca gattattttc aaagggttta tgtcgtccac cacctaacgg
    11341 agcccatgct tcaggctgca cgttataata cttcatggtt gccagcgcct ctggttgggc
    11401 aaaataggga tgcaactcga cctggttgac catcggttta actcttaccg tttcacaaaa
    11461 gttcgccaga acatgaggat agaaattgga aaccccaatc gcttttaatt tgccctcttc
    11521 ataggcatct tcgagtgcac gccacgcact gaaataatcg cgcatagcct gatgcaataa
    11581 atagaggtcg aagtattcta gtcccgattt ttttaatgat gcttcaatac ctgctgctgc
    11641 cgtatcctga ttcaacatat cctgcaccca caatttagac gttataaata actcttctcg
    11701 ggtacataaa ccttcggata tagcttcacg aacggcttca ccgacggcat cttcattacc
    11761 gtataccgct gcagtgtcga tgagtcgata ccccgtgcgg atagcgttca acactgactg
    11821 tttgtacacg tttttatcgg taacctgaaa aacaccaaat cccatcatcg gcattttcag
    11881 attgttactt aatacggaaa attccactgt cagtccctcc attaggcatg aacaatgagt
    11941 ctacgttaaa acgtaacctc aaattagtat gtggattttg atatcactta tgcaaaaaat
    12001 tcattaataa tgtaggactg aaacctctct attttcgggg acaacgaagc agacgctacc
    12061 agtgcttttg ccttcgccct tgctgttttt gatacactta gggcccaggg cataacgaaa
    12121 atgtgcgata tgacaaagaa ttaacggaga atgtgatgat caggcagaag attctacagc
    12181 agctcctgga gtggattgag tgcaatcttg agcaccctat ttcaatcgaa gatatcgcac
    12241 agaaatctgg ctacagcaga cgcaacatcc agcttctgtt ccgaaatttc atgcatgtgc
    12301 ctttgggaga atacattcgc aaacgaaggc tttgtcgtgc cgccattctt gtccggctca
    12361 ccgcgaaatc tatgcttgat attgcaatct ctttgcattt tgattcacag cagtcattca
    12421 gccgcgaatt taaaaagtta ttcggctgct ctccccgtgt ataccgccac cgtgattatt
    12481 gggatctcgc aaatatcttc ccttcttttt taatacgtca acagcaaaag acggagtgta
    12541 gattagtcaa ctttcctgag acacctattt ttggcaattc atttaaatat gatattgaag
    12601 tgtcgaataa atcaccggat gaagaagtca aactgcgacg tcatcattta gccagatgta
    12661 tgaagaattt taagacggat atctatttcg tctccacgtt tgaaccgtca acaaaatcgg
    12721 tcgatttact cacagttgaa acttttgccg gtacggtatg caaacaaacc gattcagaga
    12781 tgccgaaaga gtggacaatt aaccgaggac tgtatgcctc tttccgctat gaaggtgagt
    12841 gggagcacta ccctgaatgg gcgcgaaacc tctatctgat ggaattaccc gccagggggt
    12901 tagccagagt gaacggcagt gatattgagc ggttttatta caatgaagat ttcgtagaaa
    12961 aggatagcaa tgatgttgtt tgcgaaatat ttatccctgt tcgtccggtt tagctgtcgc
    13021 tatatctaaa tgaacttata acgagactac tgtcaggcaa ttcaataaat aatggcgttg
    13081 aagatgtcaa cgccattatt gtttattaga atgttacttc catattctta atattagttc
    13141 atcgcctcct cgccctttgc cgtatcggca tccaccgcgg taatggtcac cgtcactggc
    13201 ttcatttcgt acccgccata cgtcagggcg ttaaatgtga acgtatagac accaggcttg
    13261 ctggtactaa atgtacgcgt gatttttccg ctcacgaaat ggtcattttt cgacggcagg
    13321 aactgataat ccttctccgt caccccttct ggcgtcttaa tgtcgaccca catattcccc
    13381 ccaacggggt tatttgccga catcagtgtc acctccagct tcgcagtcga tatcccatcc
    13441 gccaccagag aagtcacatc agatgatgcg gtgagtactg catttttatc atccgcgacg
    13501 aatttagatt cggcagtttt cgtcgattcc tgaccgtcgg cctgactcac tttcgccgtg
    13561 agtgtatatt tcgctgcgac agtggtcgtg gcggtgaaaa tagcctgtcc ttgctcatta
    13621 gttttcgccg tcccattggg agtcagaact aaatttgccg ggctggcagt cagcgtcact
    13681 tcgctatctt tcaacaggtt gttattggca tcagtcaccg taactttata cgtctgtttg
    13741 gcattgccgt ccgccagtgc atagttgcca atcacctcaa taccggagac ggttgcggta
    13801 gccttatcgg ctacaaacgt tatagtctgc gtattcgaag gctggctatt ccctgccgat
    13861 gccgtgatct catacgttcc ggcagtcact gaaaccacct gcaactctgc tttcccctca
    13921 tcgttggctt tcacccagac attatcgcct gtaagcggct tgacaccccg gggcagatta
    13981 aaggtcacca ggctccctgg aacagggtta ccatttttat cctcaacgtt cgccgtcagc
    14041 gtaaaggcat ctttgccgtt tgccactttt tgagcagcgg cgtctacctg caggtttgcc
    14101 tgaccggtgc tggcatccgc gttgaatttc accgtgaccg tcttctgagc accattcacc
    14161 gaagcggaaa tattgtgttc tcccgcaacc gttgacataa gctcaatgtc cgctttacct
    14221 gccgcattag ttgttactgt attccccgtc ttgctggtca caccctgcgg taaagtcagc
    14281 gtaacttcct gcccctgcaa cgggttacca taggtgtcca caacggtcag ggttatctgg
    14341 ttagcagact gtccattagc cagttggtta ttaaccttca ctgtcatatc acgaatctca
    14401 gccttagatg cgtcacctgc aacgttcagc accagtggct gagcaacggc attttggccg
    14461 ttcactcgcg gcatcacaga caactgaccc gcggcagatc ccagcgttaa ggtcgacacg
    14521 tagaccccat tacctttctc tgtccagttt cctgctgaag gacgctcact ccccgtgctg
    14581 gcggcaccgc taaacactgg tgcatctggc ttcagcccgg tgaccgggtt cccgtacgca
    14641 tccttcacgg tgaaggtgag ttccgtcgtc gttttgacgg tcggagcctt attgtccgca
    14701 acaagcgtag agttcgctga tgacatctct ccggcgatga ccgtcaactg cgcggcttcg
    14761 gtggctgctg gctggccgtt gaagagaggc atgacgcgaa gctcgcccgt ctttccacct
    14821 gtagtcaacg tagcaacata ggaaccgtta cctttttcgg tccaactgga aacggtcgcc
    14881 ccttcagagg cggtccccgt caaacttgcc gacaacgcaa gaccactgat agcgttgcca
    14941 tgcgcatctt tcgccaccag cgtcacggtt gtgctttcgc cggcttttac gtgatcttct
    15001 gcgacagaga ctttcgactg gtttgaagat aacgcatcag ccaccacggt tacttttgcc
    15061 gcatttgctg ccgcattctg tccatttagc ttcggcataa cttctaattc acccgccgta
    15121 gagccgagag taatctgcgc agtccacgtc ccatcaccat tatttgtcca gcccgatgcc
    15181 gtagaacctt cagcagcggc acccgctaat gacggcgctt ccggcgtgag gctggtcaca
    15241 gggttgtcat aggcatcttt taccgtccag attgccgtaa ctgtcccccc aaccaccggt
    15301 ttatcaggat tcagggtgat ggacgaatgt gctgcatcaa gcggcccggc aacaaacttc
    15361 agcgtttgtt gtaatgtgcc cagctgatac tgctcgctga aagcacgcac aacaacgttt
    15421 ccggcacggg tcgaagaaac cgcggcgctg taaacgcctg gttttatttc cgaaatggca
    15481 ccaacggtta caccattagt gtcttgcgga acaaatcgca agcggctggc ttctcccgtc
    15541 accggattac cctcggagtc caccgcagtc aacgtcaacg tataggcttg ctgaccatca
    15601 gcaaccacgt cacctgacgg ctcgttagcg ctcagggtgg agtttgccac atccatcatc
    15661 gtggcccgca gttctgcagt gacggttttg ctcatgccat caacgctaac agtaatcgtt
    15721 gcctcacctg actgcgttcc ggtagtaaag acagactgat acacccctgc ttcagtttcg
    15781 gtgaactcac ccagtgttgg ctttgcctgt gatttagtgg ccttcaggga acgagtcaca
    15841 atatttccag ccggtttgaa agttagttca gtcttgatct gatctttcat gcccgtgact
    15901 ggctggccct cggcgtcgcg cagagacagc accagcggtt tttgctcatt accgttagca
    15961 agcatttgaa tacggctctg accgtcaagc gttaacgccg tgcgatcggc gctcatacca
    16021 gctccggtaa tgaccacctc tgtctgcacg cgttttgagg tattgccttt gttatcgtag
    16081 gcaactgctg aaatcgcata ataattgtct ttgcctggac gataagccgg gagcgttact
    16141 tgccactgac taccctgacc ggtaattttg ccaccttcag ccagtaatga cggcgcttcc
    16201 cactgcacat ttttcagtcc gtgagttgct ttgctgacca caagccccag ggaaagtgtc
    16261 tgaccgccct taccttcaat acgctcaggc agagcaatac ggatcacttc agatttgcgg
    16321 tactcaagaa cgatgttgtt attacgctca accaggtcat agcggctgcc tgccagtacc
    16381 cgacgctcgc gaatgctatc cgtatcgagt tgtttcgcca aaggttcgcc aattcggtag
    16441 ttaacttcca ggccaaagcg agtgtcattc tcaccgctct tgccctgctt atgcccggcg
    16501 ctcagtgtca gaagaggcac tggcgtatag gtcacctcgg cagaaatagc atgcgggtct
    16561 ttctggcgct tatctttacc aaacagcccg acttcatcgc cataatactg ttcatacatc
    16621 aggcttgcgc caagctgcgg ccaggcaggt aaatagccct ctgcgcggat atcccaacca
    16681 ttcgccgggc gttcctgata atcctcaata tccggcgatt ttttccagcc agaagcccga
    16741 atataaccat tggcgctcag tttcagataa tcgcgccagt attccgcacc aacaccaatg
    16801 cgggtatgac tacgggataa atcatggtcg ataaaggtgt tcacccccgc catccagtca
    16861 tttcctgaaa aatgacgcca gccaaaacca atatttgact gagtacgatc gtctgtacga
    16921 tgtattgccc cctgagtgaa caacatattt gtcggcgtat cataaatcgg ataaagcatt
    16981 tccagcgaag aatccttcag cgagaaatct ttatcgacat tcagtttgac gcgcgctgta
    17041 ccatatttcc cgagccactc ctgtatttcc tggttagctt tagcggtggc cattccggta
    17101 ataaaattac gtgtcgcatc gctatctggc tgactgctta aaaatgtccc ggcatttgcg
    17161 gcaaacgacg cgacattttt ctccacgtta ttatcagcag ttaccgtagt atttcccatg
    17221 ctcaaccgtg gctgaaccgc atgctgcgca cgtgccgcca ttactggggt aaaggtgaca
    17281 gcgagtggaa aaagaacctg aacagagata tttgcccacg ccacgcagcg ggccagaact
    17341 gaataacgaa atcgtggttg tttatgacct gttttataac gtgacattga gataatttcc
    17401 tgatgaacga attaacgaac ataggcagac acaaataatg gcgtctgctt gtcagaaaaa
    17461 ccggggattt cccggtttcc ttttccgcag aaaaggaaat acctttatgt aagaaagtac
    17521 accgccacct aatatatcca gtgcctgtag ccactcatca ggtggttggg cgcgggatta
    17581 tatttttatt tatgggtgaa aatttagctt tttgtgcaag tcattaaata gatacttaag
    17641 ttatatttct tactctgcgg acagtgaata attcattatt cagagtgaat aataatgacc
    17701 tgcctttaat tagcaactat tggaaatacg tcgaagtagg tatatacgcc aggataaacc
    17761 gatatatttt ttatggatat tcattttcct tcatcggact gtaaattgcg gagatatcga
    17821 acttgcacta cccatcgcaa cactcaaaca gatttatgaa tttcgttcat aacccctgac
    17881 aaatcctgcc gtctaatcga gctcttctct ttacgttatg cttctcatga acgataacgg
    17941 aaggataaca ccatgcaaaa agtaaaactg aataacggta ttgaaatgcc cctgctgggc
    18001 tttggtgtat ttcagatgac cgatgccgcc gaatgcgaaa gagcggtcat tgacgcta
//
LOCUS       DAZGXS010000095        17713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGXS010000095 DAZGXS010000000
VERSION     DAZGXS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            271..1614
                     /locus_tag="ACHKG5_003315"
     CDS             271..1614
                     /locus_tag="ACHKG5_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869356.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1763..3271)
                     /gene="putP"
                     /locus_tag="ACHKG5_003316"
     CDS             complement(1763..3271)
                     /gene="putP"
                     /locus_tag="ACHKG5_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP0869357.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3430..3639)
                     /locus_tag="ACHKG5_003317"
     CDS             complement(3430..3639)
                     /locus_tag="ACHKG5_003317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869358.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3694..7656
                     /gene="putA"
                     /locus_tag="ACHKG5_003318"
     CDS             3694..7656
                     /gene="putA"
                     /locus_tag="ACHKG5_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0869359.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7696..8334)
                     /gene="rutR"
                     /locus_tag="ACHKG5_003319"
     CDS             complement(7696..8334)
                     /gene="rutR"
                     /locus_tag="ACHKG5_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0869360.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8622..9713
                     /gene="rutA"
                     /locus_tag="ACHKG5_003320"
     CDS             8622..9713
                     /gene="rutA"
                     /locus_tag="ACHKG5_003320"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0869361.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9713..10405
                     /gene="rutB"
                     /locus_tag="ACHKG5_003321"
     CDS             9713..10405
                     /gene="rutB"
                     /locus_tag="ACHKG5_003321"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP0869362.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10417..10803
                     /gene="rutC"
                     /locus_tag="ACHKG5_003322"
     CDS             10417..10803
                     /gene="rutC"
                     /locus_tag="ACHKG5_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0869363.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10811..11611
                     /gene="rutD"
                     /locus_tag="ACHKG5_003323"
     CDS             10811..11611
                     /gene="rutD"
                     /locus_tag="ACHKG5_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0869364.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11621..12211
                     /gene="rutE"
                     /locus_tag="ACHKG5_003324"
     CDS             11621..12211
                     /gene="rutE"
                     /locus_tag="ACHKG5_003324"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0869365.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12222..12716
                     /gene="rutF"
                     /locus_tag="ACHKG5_003325"
     CDS             12222..12716
                     /gene="rutF"
                     /locus_tag="ACHKG5_003325"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0869366.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12737..14065
                     /gene="rutG"
                     /locus_tag="ACHKG5_003326"
     CDS             12737..14065
                     /gene="rutG"
                     /locus_tag="ACHKG5_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP0869367.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14148..14255)
                     /locus_tag="ACHKG5_003327"
     CDS             complement(14148..14255)
                     /locus_tag="ACHKG5_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFP0869368.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14627..15223
                     /gene="wrbA"
                     /locus_tag="ACHKG5_003328"
     CDS             14627..15223
                     /gene="wrbA"
                     /locus_tag="ACHKG5_003328"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0869369.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15244..15471
                     /gene="yccJ"
                     /locus_tag="ACHKG5_003329"
     CDS             15244..15471
                     /gene="yccJ"
                     /locus_tag="ACHKG5_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0869370.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15509..16750)
                     /gene="agp"
                     /locus_tag="ACHKG5_003330"
     CDS             complement(15509..16750)
                     /gene="agp"
                     /locus_tag="ACHKG5_003330"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0869371.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(17041..17713)
                     /locus_tag="ACHKG5_003331"
                     /pseudo
     CDS             complement(17041..17713)
                     /locus_tag="ACHKG5_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4250 a         4686 c         4618 g         4159 t
ORIGIN      
        1 tattatttaa cgcgatgata aaaggcgtaa aaaaaataat aatcttcatg aaactcaccc
       61 cgatggcagc attgtaaatt cgccaataac tggcattcct cctgcttttt acgggtgttt
      121 ccattaacac cagaatgagc ggattgttta ctacatcaag aacgcatccg aagtgtacat
      181 cctggtgcgc cgactgaatc gcccggagcc atgcggatgg ctacgactta atacgaatca
      241 ttaaccctgt aaggatacgg agagcattac atgagaccaa tatccaacct caatacgagc
      301 gcagcaacct atattccacc gcaacaactc ccgagtagtt gtctggaaac tttgacgctt
      361 ctggtgcaga tgaataatta tatcaaaagg gatgcggact acagcacggg catggctgta
      421 gctccactgg ttcctgaaga ggttcacttg ctggctgctg cgatgacaac cgaactgcat
      481 cgacatcagt tccaaccggt ggtaagtgca aatgatctgc aacttcctga accgttcacc
      541 ttcgatgttg ccggtttcac catcaccttc acgaagacgc aagagcatga caacacgggc
      601 aatctcatga aaattgcagt cagcaagagc ggtgtgtgca ccagtacaaa tattactctt
      661 gagctgtttc actccattgt cacaaccctg atgtcacgca gtcaatatgg aacctttgat
      721 ctctggtccg tacggccaat actcacggat gaaagtcaaa acagggtgca tgaagcagct
      781 cgctactccc cggcccagca atacggaaga gaagaaacac actttaccaa caatggcatg
      841 cgagaatttg gaaatatgtc tccgctggcg tggcgtaacg atgtggaatt acagcaatcg
      901 tgtaatacaa gcaacatccc accaaatgct gcaaatgacg acataaacaa caccaggcag
      961 accatcgagg accagccaga ggcggacgag ccacagcaat atgttaaatt gactgtagac
     1021 gacatgcgaa aatgggcggc tatggaccag caggcgcgaa acgctctaca gggagtttca
     1081 ggatggtgta cgcgcaatca ttttaatatc aaaaatgcca gaaattacct gaccgatcac
     1141 ggcctgaact acgcgggaca ggtaaaggta aacaggccgc atgaatatgc caaattcacc
     1201 cttgaacata tccgccagtg ggcagcctta aacaagcatg tgcgaaaacc ggttggttat
     1261 ttagaaaagt ggtgtaagga aagaaacctc gcgccaacta ctgccaggaa ttacctgaag
     1321 aacgacgggc ttaccgccct gggtgagttg aagctgacag ggccgcagaa atgggtcacg
     1381 ttcaccttcg gagacatcgt acaatgggcg aatatgaccc aggaggagcg taacagcgca
     1441 gggggagccg aaaagtggag taaaaaacgc ggcttccagt ggtctactgc cagatcctac
     1501 ctgaaatcga gcggagtgac caagcaaggt gcgagaaaac tggcgtggtt aaaaaacagc
     1561 gggaatatgt cgaatccatt ctatctcgcc caaccaacca gccgcaggac ataagctgaa
     1621 ggtaccgata gcggcaattt tgttcatctg tgccgggtgg gcaaatagca attgcccccg
     1681 aacactccct gccaggcaac ttataccgca tggcagggaa cattcgggtt aaaaagcaaa
     1741 agataactaa ttactcttca gcttagcctt cctgcaaccg tgacggcgga gccgaatgat
     1801 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggtgct ttacccagca
     1861 aactaaacac tacaatccca atactgccaa agataaagcc cggaataatt tcgtacagtc
     1921 ccagccagcc gaactgtttc cagacgatca ccgtcagcgc accgatgatc atccccgcca
     1981 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgaaaa cagcaccacc ggaccaaacg
     2041 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggttcg
     2101 ctgccaacgc aatcgccacc agcgccacca ccagcaccat cacacgccct acccacacca
     2161 gttctttctg gctggcatgt ttacgcagaa acgctttgta caaatcttcg gtaatcgcac
     2221 tggaacatac cagcagctgg caacttaacg ttgacattac cgccgccaga atcgccgaca
     2281 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgct
     2341 cggcgttctg gtttaccgca ccagctaccg acgggtgctc gttaaagtaa gcgataccaa
     2401 agaagccgac agccaccgcc cctgccaggc agaggatcat ccaggtcata ctgatacgac
     2461 gcgcatggac gatgctgtgg tgagaatccg ccgccataaa acgcgccagg atgtgcggtt
     2521 gcccgaagta acccagcccc caccccatca gcgagataat ggcgacaaag ttcagccctt
     2581 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
     2641 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     2701 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     2761 tcgccgcagc tcccgcccac agagccgttt cgtagctcat gccaaaggta ctttcaaaca
     2821 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaatcacca
     2881 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     2941 gtaatgttaa ggcgttattg ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     3001 tccagttaat ccacgcgcct aatgtcaggc caatggcgat ccaactttcg gaaatcccgg
     3061 aaagaaaaac cgcgcccggc aaccccatta acagccagcc gctcatatcc gatgcacccg
     3121 ccgataatgc cgtcacgaat ggcccaaggc tacgtccgcc cagaatatag tcgtcaaagt
     3181 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     3241 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     3301 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     3361 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     3421 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     3481 tgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgag ttattttttt
     3541 aatccctgtc atatcgattt cttttattaa catttcattc atttttaagc ttgctacgca
     3601 ggtcacattt aacatggttg cacaaagttg caacatcatg gatatttcac gataacgtta
     3661 agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat gggggttaag
     3721 ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga tcgcacgcca
     3781 cactggttaa ttaagcaggc gattttttct tatctcgaac aactggaaaa cagcgatact
     3841 ctgccagagc tacctgcgct gctttctggc gctgccaatg agagcgatga agcaccgact
     3901 ccggcagagg aaccacacca gccattcctc gactttgccg agcaaatttt gccccagtcg
     3961 gtttcccgcg ctgcgatcac cgcggcctat cgccgcccgg aaaccgaagc ggtttctatg
     4021 ctgctggaac aagcccgcct gccgcaacca gttgctgaac aggcacacaa actggcgtat
     4081 caactggccg ataaactgcg taatcaaaaa aatgccagtg gccgcgcagg tatggtccag
     4141 gggttattgc aggagttttc gctgtcatcg caggaaggcg tggcgctgat gtgtctggca
     4201 gaagcgttgt tgcgtattcc cgacaaagcc acccgtgatg cgttaattcg cgacaaaatc
     4261 agcaacggta actggcagtc acacattggt cgtagcccgt cactgtttgt taatgccgct
     4321 acctgggggc tgttgtttac tggcaaactg gtttccaccc ataacgaagc cagcctctcc
     4381 cgctcgctga accgcattat cggtaagagc ggtgaaccgc tgatccgcaa aggtgtggat
     4441 atggcaatgc gcctgatggg cgagcagttc gtcacgggcg aaaccatcgc ggaagcgtta
     4501 gccaatgccc gcaagctgga agagaaaggt ttccgttact cttacgatat gctcggcgaa
     4561 gctgcactga ctgccgcaga cgcacaggcg tacatggttt cctatcagca ggcgattcac
     4621 gccattggta aagcgtctaa cggtcgtggc atctatgaag gtccgggcat ttcaatcaag
     4681 ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat ggaagagctt
     4741 tacccgcgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg tatcaacatt
     4801 gacgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa actctgtttc
     4861 gagccagagc tggcaggctg gaacggcatc ggttttgtta ttcaggctta tcaaaaacgc
     4921 tgcccgctgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg ccgtcttatg
     4981 attcgcctgg tgaaaggcgc gtactgggat agtgaaatca agcgcgcgca gatggacggc
     5041 cttgaaggtt atccggttta tacccgcaag gtatataccg acgtttctta tctcgcctgt
     5101 gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcaac gcacaacgcc
     5161 cacacgctgg cggcgattta tcaactggca ggtcagaact actatccggg tcagtacgag
     5221 ttccagtgcc tgcatggtat gggcgagccg ctgtatgagc aggtcaccgg gaaagttgcc
     5281 gacggcaaac ttaaccgtcc gtgtcgtatt tatgccccgg tcggcacaca tgaaacgctg
     5341 ctggcgtatc tggtgcgccg cctgctggaa aacggtgcta acacctcgtt tgttaaccgt
     5401 attgccgata cctctttgcc actggatgaa ctggtcgccg atccggtcac tgctgtagaa
     5461 aaactggcac aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc
     5521 gatctttacg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa cgaacaccgt
     5581 ctggcatccc tctcttctgc cctgctcaat agtgcactgc aaaaatggca ggccttgcca
     5641 atgctggaac aaccggtagc ggcaggtgag atgtcgcccg ttattaaccc ggcggaaccg
     5701 aaagatattg tgggctatgt acgtgaagcc acgccgcgtg aagtagagca ggcgctggaa
     5761 agtgcggtta ataacgcgcc aatctggttt gccactcctc cggctgaacg cgcggcgatt
     5821 ttgcatcgcg ctgccgtgct gatggaaagc cagatgcagc aactgattgg tattctggtg
     5881 cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgtgaagc ggtcgatttt
     5941 ctccactact acgccggaca ggtgcgggat gatttcgcta acgaaaccca tcgtccatta
     6001 gggcctgtgg tatgtatcag cccgtggaac ttcccgctgg cgattttcac cgggcagatc
     6061 gctgccgcac tggcggcagg taacagcgta ctggcaaaac cggcagaaca aacgccactg
     6121 attgccgcgc aagggatcgc cattttgctg gaagcgggcg taccgccagg cgtggtgcaa
     6181 ttgctgccag gtcggggtga aaccgtgggc gcgcaactga cgggtgatga tcgcgtacgc
     6241 ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa tatcgccagc
     6301 cgcctcgacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg catgaacgcg
     6361 atgattgtcg attcttcagc actgaccgaa caagtcgtaa tagatgtgct ggcctcggcg
     6421 ttcgacagtg cgggtcagcg ttgttcggcg ctgcgcgtgc tgtgcctgca agatgagatt
     6481 gccgaccaca cgttgaaaat gctgcgcggc gcaatggccg aatgccggat gggtaatccg
     6541 ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagc caatattgag
     6601 cgccatattc agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt gcgggaaaac
     6661 agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct gatcgaactg
     6721 gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt ggtgcgttat
     6781 aaccgtaatc agctgccgga gctgatcgag cagattaacg cttccggcta tggtctgacg
     6841 cttggcgttc atacgcgtat tgatgaaacc atcgcccaag tcactggctc agcccatgtt
     6901 ggtaacctgt atgttaaccg taatatggtg ggcgcagtgg ttggggtgca gccattcggc
     6961 ggcgaagggt tgtccggtac cgggccgaaa gcaggcggtc cgctctatct ctaccgtctg
     7021 ctggcgaatc gcccggaaag tgcgctggcg gtgacactcg cgcgtcagga tgcagagtat
     7081 ccggtcgatg cgcagttgaa agccgcattg actcagccgc taaatgcact gcgggaatgg
     7141 gcagcaaatc gtccagaatt gcaggcgtta tgtacgcaat atggcgagct ggcgcaggca
     7201 ggaacacaac gattgctgcc agggccgacg ggtgagcgca acacctggac gctgctgccg
     7261 cgtgagcgcg tattgtgtat cgcggatgat gaacaggatg cgctgactca gctcgccgcc
     7321 gtgctggcgg tgggcagcca ggtgctgtgg ccagatgacg cgctgcatcg tcagttagtg
     7381 aaggcattgc catcgactgt cagcgagcgt attcaactgg cgaaagcgga aaatattacc
     7441 gctcaaccgt ttgatgcggt gatcttccac ggtgattcgg atcagcttcg cgcattgtgt
     7501 gaagcagttg ccgcgcggga tggcgcaatt gtttcggtgc agggttttgc ccgtggcgaa
     7561 agcaatatcc tgctggaacg gctgtatatc gagcgttcgc tgagtgtgaa taccgctgcc
     7621 gctggcggta acgccagctt aatgacgatc ggttaaacag gtagccggag gatgttacac
     7681 cctcctccgg catcattagc gtggtcgaat cccctcaata ataatccgct gcacgttttc
     7741 aaccgtttga ttgaaaaata cctcatcgcg caacgttgcg cctgtcaccg cctccacctg
     7801 aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc ataaaaatca aatgctgcgg
     7861 atcaatcggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg atttctcatc
     7921 aattaatgac ttcaaatcgc ccgtcagttc atccattaac agcggcgcgc ctgccagcat
     7981 ctccatacag aacaggcgcg aagcctgcgg ataatcgcgt gagacttcca gcttcagacg
     8041 gatgtactct ttgatcgccg ccagcggggc gaaatcttca cgaaacgctt ttaacggtgc
     8101 cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt ttgacggaaa
     8161 gtaatacagc agattggttt ttgaaacacc cgccaactct gcaatttgct ccagccgtgt
     8221 gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt
     8281 cgcgcttact gcgcgcgaac gtttacccgt tgttttcact gcgccttgcg tcatgcgctc
     8341 tcccctcttg tagattggca acaggatagc aaaacccccg cccggcctcg accttctgca
     8401 ctctcatcgc gcacagatgc atgttttatg tgcaactgtt ttgaccgttt agtccacttt
     8461 ttaccagaca ttttagataa cgcgatgtta acacattaat aacactccct ttttaaaaac
     8521 tggcatccgc tttgcaaaca agccaatacg cggctgaaaa ggatatgcag aatgcagcgc
     8581 cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt ggcgtattcg
     8641 tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac atgccgacct
     8701 ttgaactgaa taaagccatc gtgcaaaaag cggagcacta ccatttcgat ttcgccctgt
     8761 cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac aaccttgagt
     8821 cgttcacctt gatggcgggg ctggcggccg tgacctcgcg cattcagatt tacgccaccg
     8881 ccgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc atcgactcca
     8941 tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagccc gagtatgagc
     9001 agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat ctcaccgagt
     9061 atgttcaggt gctgcgcgac ctgtggggca cgggaaaaag cgattttaaa ggcgattttt
     9121 tcaccatgaa tgattgtcgc gtcagtccgc aaccaagtgt ccccatgaaa gtgatctgcg
     9181 ccgggcaaag cgacgctggc atggcgttct ccgctcagta tgccgatttc aacttctgtt
     9241 tcggcaaagg cgtaaataca cccacggctt tcgccccgac cgctgcgcgg atgaaacagg
     9301 ccgcagagca aaccgggcgc gacgttggct cttatgtatt gtttatggtg attgccgatg
     9361 aaaccgacga tgccgctcgc gccaaatggg aacactacaa agcgggcgcg gatgaagagg
     9421 cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccggtact gacaccaacg
     9481 tccgtcagat ggccgatccc acttcggcgg taaacatcaa tatggggacg ttagtcggtt
     9541 cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt gccgaaggcg
     9601 tgctgttaac cttcgacgat tttctgtcgg gaatcgaaac cttcggcgag cgcattcaac
     9661 cactgatgca gtgccgcgcc catctccctg tgctgactca ggaggtggca tgatgacgac
     9721 attaaccgct cgaccggaag ccattacctt cgatccgcag caaagtgcgc tgatcgtggt
     9781 ggatatgcaa aacgcctatg ccacgccagg cggctactta gatctcgccg ggtttgatgt
     9841 ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcag cgcgagcggc
     9901 agggatgttg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg aggctggcgg
     9961 ccctggctca ccgaattttc ataaatcgaa cgccctcaaa accatgcgta aacagccgca
    10021 gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg atgaactggt
    10081 gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct tcaatacgcc
    10141 gctggacagc attttgcgca gccgcggaat acgccatctg gttttcacca gcatcgctac
    10201 caacgtctgc gtcgaatcga cgctacgcga tggctttttt ctggagtatt tcggcgtggt
    10261 gctggaagac gcaacacacc aggcggggcc ggaatttgta cagaaagccg cgttgttcaa
    10321 tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg cgctttctcc
    10381 cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt aattattccc
    10441 gctggcagca gcgcaccgct ggcccccttc gttcccggca cgctggctga tggcgtggtg
    10501 tatgtctccg gtacgctggc ttttgatcaa cacaataacg tgctgtttgc cgatgaccca
    10561 aaggcgcaaa cccgccacgt tctggaaact atccgcaagg taatcgagac agcgggtggc
    10621 acgatggcgg atgtgacctt caacagcatc tttattaccg actggaaaaa ttacgccgcg
    10681 attaacgaaa cctacgccga gttttttcca ggtgataaac cggcgcgatt ctgcattcag
    10741 tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca tatcgccaag
    10801 tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc ccgtagtggt
    10861 gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg cggtgctgga
    10921 gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc ccgacacgct
    10981 ggcagaagat tacagtatca cccagatggc agcggaactg catcaggcgc tggtagccgc
    11041 agggattgag cattacgcag tggtcggcca tgcgctcggt gcgctggtgg gaatgcagct
    11101 ggcgctggat catcccgcgt cggtaactgt gctggtctgc gttaacggct ggctacgaat
    11161 aaacgcccat acgcgccgct gttttcaggt tcgcgaacga ttactgtata gcggtggcgc
    11221 gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga tggcggcccg
    11281 cgcacctcgc cttgaggcag aagacgcgct ggcactggcg cattttcagg gcaaaaataa
    11341 tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg cggatcgcat
    11401 ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccat cagcatgttc
    11461 cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgcgct atggcggaca
    11521 cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg ggcttgccag
    11581 cctgttacat caccgtgaag ccgccctgta aggaattgct atgaacgaag ccgttagccc
    11641 aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac aacggctggc gggagacacc
    11701 cgtcagcgat gagacgttac gggagcttta tgccctgatg aaatgggggc cgacatcagc
    11761 taactgttct ccggcacgta tcgtgtttat ccgcactgca gagggaaaag aacgtctgcg
    11821 cccggcgctt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca ccgctatcgt
    11881 cgcatgggac agtgaatttt atgaacggtt accgcaactg tttcctcacg gcgatgcccg
    11941 cagttggttt acctccagcc cacaacttgc cgaagaaaca gcgtttcgca acagttccat
    12001 gcaggcggct tatctgatca tcgcctgccg tgcgctgggg ctggataccg gcccgatgtc
    12061 gggttttgat cgtcaacacg tagacgacgc tttttttgca ggcagcacgc tgaagagcaa
    12121 tctgctgatt aatatcggct atggcgatag cagcaaactt tttgcgcgcc tgccacgtct
    12181 gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac tatgaacatt gtcgatcaac
    12241 aaacttttcg cgatgcgatg tcctgcatgg gcgcggcggt caatatcatc accacggacg
    12301 gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg cagcgtgacc gacacgcctc
    12361 ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc aatgaaaatc
    12421 gaacgctgtg tgtaaataca cttagcgccg ggcaggagcc gctttcaaac ctttttggcg
    12481 gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagactggt gtgaccggat
    12541 gcccgcaact ggaagaggcg ctggtttcgt ttgactgccg tatcagccag gtggtgagcg
    12601 tcggcaccca cgacattttg ttttgcgcca tcgaagcgat tcatcgtcac gccaccccct
    12661 acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct tgttaacctc
    12721 cttaaggaga cagctgatgg caatgttcgg tcttcctcac tggcagttaa aatcgacctc
    12781 tacagagagc ggcgtggtcg cgccggatga acgattgccg tttgctcaga cggcagtaat
    12841 gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccga ttctgatggg
    12901 gctggatccc aatctttcca ttttaatgtc agggatcggc acgttactgt ttttctttat
    12961 taccggcggg cgcgtgccca gttatctcgg ctccagcgcg gcttttgtcg gcgtggtgat
    13021 cgccgcaacg ggctttaacg gtcagggcat taacccgaat atcagcattg cgctgggcgg
    13081 gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga aaatcggtac
    13141 gcgctggatt gaacggctaa tgccgccggt ggtgacgggt gcggtggtga tggcgattgg
    13201 cctgaactta gcgcctatcg cggtaaaaag tgtatcggct tcaggctttg atagctggat
    13261 ggcggtaatg acggtgctgt gtattgggct ggtggcggtg tttacccgtg gcatgatcca
    13321 gcggttattg attcttgtag gattaattgt cgcctgtctg ctgtatggcg tgatgaccaa
    13381 cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg cctggttcgg
    13441 tctgccacat ttttccactc ctgcttttaa tggtcaggca atgatgctga ttgcgcccgt
    13501 ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag gcggtcgccg ggatgaccgg
    13561 acgcaatatg gacccgtata tggggcgagc attcgtaggc gatggtctgg caacgatgct
    13621 ttctggttct gtcggcagta gcggcgtcac cacctatgct gaaaatatcg gtgtgatggc
    13681 cgtgacaaaa gtctactcga cgctggtgtt tgttgctgct gccgtcatcg ccatgctgtt
    13741 gggcttttca ccgaaatttg gcgcgctgat tcataccatt ccggccgcgg tgattggtgg
    13801 tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcaaggatat gggtacaaaa
    13861 ccgtgtcgat ttaagccaga acggtaattt gattatggtc gcagtaacct tagtgctggg
    13921 cgcgggtgat tttgctctca cgctgggcgg ttttacgttg ggaggaatcg gtacagcaac
    13981 ctttggcgcg attttactca atgcgttatt aagcagaagg ttggttgacg ttccaccgcc
    14041 agaagttgtt catcaggagc cataaacaga aaaccgccgg ggttaccggc ggtgatggct
    14101 ttttgacgag cttaagaaaa aaatgcattc gtcattgatt agcccggcta gttgtcgctt
    14161 ttgccgtgac tgctcttgcc acctttttta cctgcttctg atgctctttc gcggtcttct
    14221 gcaaaattgc cggaaccgcc tcgatggttt gccattactg acctccgtct gggtatagac
    14281 atcatattgc tagaattaac gcggcgtatg gcggctgcca tattttcgct acgtgggact
    14341 aagcttagtg aactgacgtg tttctacccg cgtttggtaa tatcctgcaa cagcttctta
    14401 gcgagaatag atatcatctc caataattat ccataagccg ctcttatgat taaaaaaacg
    14461 cgatgagata aaaatgtgtc attttgcgac aaaattgcgt gcttgtgaaa gttgttataa
    14521 atcaaataag tggttgtgaa atttgcactc tgaaaaggac gtcttatctt taaataagtg
    14581 gtagcgaatc gctacggaat agagataaca cgaggagtgg ttagaaatgg ctaaagttct
    14641 ggtgctttat tattccatgt acggacatat tgaaacgatg gcacgcgcag tcgctgaggg
    14701 tgcaagcaaa gtggatggcg ctgaagttgt cgttaagcgt gtaccggaaa ccatgtcgcc
    14761 gcaattattt gaaaaagcag gcggtaaaac gcaaactgca ccggttgcaa ccccgcaaga
    14821 actggccaat tacgacgcca ttatttttgg tacacctacc cgctttggca acatgtccgg
    14881 tcaaatgcgt accttcctcg accagacggg cggcctgtgg gcttccggcg cactatacgg
    14941 aaaactggcg agcgtcttta gttccaccgg tactggcggc ggtcaggaac aaaccatcac
    15001 atccacctgg acgacccttg cgcatcacgg catggtgatt gtccccattg gctacgcagc
    15061 gcaggaatta tttgacgttt cacaggttcg cggcggtacg ccgtacggcg caaccaccat
    15121 cgcaggcggt gacggttcac gccagcccag ccaggaagaa ctgtctattg ctcgttatca
    15181 aggggaatat gtcgcaggtc tggcagttaa acttaatggc taatcttcaa caggaggata
    15241 cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg aatgggcatc tctgcgcaat
    15301 acgtcgccgg aaatagccga ggccattttc gaagtcgccg ggtacgacga aaaaatggcg
    15361 gaaaagattt gggaagaagg tagcgatgaa gtgttagtca aagcctttgc taagaccgac
    15421 aaagattcgc ttttttgggg cgaacagacc atcgaacgta aaaacgttta accagcgact
    15481 cccccgtagc gaacggggga gttttctgtt atttcaccgc ttcattcaac acgctatcaa
    15541 acttatccat cgggcagaaa ccatcagcgt ctatcgggca accgcttaat tccagcgtca
    15601 cgcgctgcgc aggtgcctgc agggttaacg catcggcatt acgtaactgt tccgcactct
    15661 ggtacacata ttcaattttc atcaaatcgc gattggcttt gctgtcacgc caacgctgga
    15721 aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc atgcaactga tacggtttga
    15781 aatccagcgc cgttaacaga gaggcaatgt tggagtcatg cccaactaac accgtgattt
    15841 ttggagcact ggtgcgatcg gtgaccaggg ctttgtcgat ataactgacc agcggtttcg
    15901 caacattgcg cgccacttcc ggtgaggtaa acaggctgtc ctggtaaccg tttttcagct
    15961 tcgacaacac cttccactgc tggtcagatt tgatttctcc ccaggccacc tgatccatcg
    16021 gaaaaccttc gtagtattgc aaagtaaacg catctaccag cgagttgccg actttcagcg
    16081 gcccggaaac acctggttct tgttgatact tggcgctaaa ggtatttttg ccatccacca
    16141 gcgaacactg ttgtttctct ttacaggcag gggaatcttt atagttaacg attttttcca
    16201 gtagctggta gctgtcggta agctggagtt tgctgagctc tttctccatt gccgccaccg
    16261 cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg gttaaaggtt gggtccatag
    16321 tgcccatttt ttcctggtga tgcacaggaa tatcacaccc cgggaatgcg ccggtaataa
    16381 agaactgtgc ggtcgcaacg gtacgttgca gactattggc ataggcataa acggtgtccg
    16441 gcggcgggca ttcccccgat tttaccatcc cctgctctgc cagccattca cgcatgtaat
    16501 ggcccatata cacttcgagt acgccacctt tggtggtgag ttgcccaccg gggacgtccc
    16561 attcgggcca tttattcggc gtcgactgct ccagcacact gccattgttc gccagcggcg
    16621 cacgtaagtt atggcggctc atcatgagca cttgctgtag ctgatagcct tccggtacgg
    16681 tttgtgcctg agcgtttgaa gcgagtaaaa ctacccctgc cacagctgcg gcgattagcg
    16741 ttttgttcat tcctggcacc tcttttgtta tcaatcacta aagagtgtga cagaaatatg
    16801 acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa cggctgtttt tatattcatc
    16861 tttaaaaaca ggcaaggttt gacgcaaact atgcctgatt agcccctgcc tcgcgtacgc
    16921 cgctgatgat tgcacctgtg gctaaacaga atatcggcgg cgttaccaat acagaggcaa
    16981 tatttaccaa taaaaaagtg tgagactaat aacaatggtt tttagcatat gcctctgtca
    17041 ttaaattgca taacgagtta aaaaaccaat ttgcgcattc ctttccttat tgtttaactc
    17101 gtaagcctga gcaaaaagtt caggagggag gtaaacgtca taatgattgc cagtgtttat
    17161 taaataacca tactcaagat cttcatctgt tgaaaaggat tcattcccta aggagattga
    17221 tgccacatta ctattaacaa taaataatga gcaaatgcta tctgaaagag cagtgaggaa
    17281 atttttcacc tcattaatcc tcttctctgg gtcggtgtct aaaacaaggc gcgccttgtt
    17341 atcattaaat ataaactcgg gtaaatgcag tatgcatttt ttgtcattca agcaactatc
    17401 aataaattct ttttttaaaa aacagaaagt tccatgacat aagactaccc tgcccctgaa
    17461 ttttgttgtc agattgtaga tcaacgccaa ataacgtctt tacatcttcc ctgagattat
    17521 agaatgccgt atcaacaaga gcctcgatcg tttgtttatc aacaatttta ataaactctt
    17581 cgtatacttg cttttgcgtc ttatggctgc ccagcgccca tgaatactca tgttctttta
    17641 aaaccagtat ggcccttaac agacaatagc catcgccatc tactggagcg tattttaatc
    17701 cataagtttt att
//
LOCUS       DAZGXS010000096        17625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGXS010000096 DAZGXS010000000
VERSION     DAZGXS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            132..320
                     /locus_tag="ACHKG5_003332"
     CDS             132..320
                     /locus_tag="ACHKG5_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0869372.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            378..932
                     /locus_tag="ACHKG5_003333"
                     /pseudo
     CDS             378..932
                     /locus_tag="ACHKG5_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            1125..1322
                     /locus_tag="ACHKG5_003334"
     CDS             1125..1322
                     /locus_tag="ACHKG5_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0869373.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            1315..1527
                     /locus_tag="ACHKG5_003335"
     CDS             1315..1527
                     /locus_tag="ACHKG5_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869374.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            1517..1981
                     /locus_tag="ACHKG5_003336"
     CDS             1517..1981
                     /locus_tag="ACHKG5_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869375.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1974..2207
                     /locus_tag="ACHKG5_003337"
     CDS             1974..2207
                     /locus_tag="ACHKG5_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869376.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            2213..2512
                     /locus_tag="ACHKG5_003338"
     CDS             2213..2512
                     /locus_tag="ACHKG5_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0869377.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            2509..3909
                     /locus_tag="ACHKG5_003339"
     CDS             2509..3909
                     /locus_tag="ACHKG5_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP0869378.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            4110..4361
                     /locus_tag="ACHKG5_003340"
     CDS             4110..4361
                     /locus_tag="ACHKG5_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869379.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            4358..4768
                     /locus_tag="ACHKG5_003341"
     CDS             4358..4768
                     /locus_tag="ACHKG5_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0869380.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            4779..5051
                     /locus_tag="ACHKG5_003342"
     CDS             4779..5051
                     /locus_tag="ACHKG5_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0869381.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            5008..5136
                     /locus_tag="ACHKG5_003343"
     CDS             5008..5136
                     /locus_tag="ACHKG5_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0869382.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            5178..5402
                     /locus_tag="ACHKG5_003344"
     CDS             5178..5402
                     /locus_tag="ACHKG5_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869383.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            5654..5860
                     /locus_tag="ACHKG5_003345"
     CDS             5654..5860
                     /locus_tag="ACHKG5_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869384.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            5860..6915
                     /locus_tag="ACHKG5_003346"
     CDS             5860..6915
                     /locus_tag="ACHKG5_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0869385.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            6928..7263
                     /locus_tag="ACHKG5_003347"
     CDS             6928..7263
                     /locus_tag="ACHKG5_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0869386.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            7276..7689
                     /locus_tag="ACHKG5_003348"
     CDS             7276..7689
                     /locus_tag="ACHKG5_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP0869387.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            7895..8437
                     /locus_tag="ACHKG5_003349"
     CDS             7895..8437
                     /locus_tag="ACHKG5_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0869388.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGRDAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            8693..8974
                     /locus_tag="ACHKG5_003350"
     CDS             8693..8974
                     /locus_tag="ACHKG5_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869389.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9575..11035)
                     /gene="phoQ"
                     /locus_tag="ACHKG5_003351"
     CDS             complement(9575..11035)
                     /gene="phoQ"
                     /locus_tag="ACHKG5_003351"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0869390.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(11035..11706)
                     /gene="phoP"
                     /locus_tag="ACHKG5_003352"
     CDS             complement(11035..11706)
                     /gene="phoP"
                     /locus_tag="ACHKG5_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0869391.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(11875..13245)
                     /gene="purB"
                     /locus_tag="ACHKG5_003353"
     CDS             complement(11875..13245)
                     /gene="purB"
                     /locus_tag="ACHKG5_003353"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0869392.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(13249..13890)
                     /gene="hflD"
                     /locus_tag="ACHKG5_003354"
     CDS             complement(13249..13890)
                     /gene="hflD"
                     /locus_tag="ACHKG5_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0869393.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(13926..15032)
                     /gene="mnmA"
                     /locus_tag="ACHKG5_003355"
                     /gene_synonym="trmU"
     CDS             complement(13926..15032)
                     /gene="mnmA"
                     /locus_tag="ACHKG5_003355"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0869394.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(15086..15547)
                     /gene="nudJ"
                     /locus_tag="ACHKG5_003356"
     CDS             complement(15086..15547)
                     /gene="nudJ"
                     /locus_tag="ACHKG5_003356"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0869395.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(15557..16210)
                     /gene="rluE"
                     /locus_tag="ACHKG5_003357"
     CDS             complement(15557..16210)
                     /gene="rluE"
                     /locus_tag="ACHKG5_003357"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0869396.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            16382..>17625
                     /gene="icd"
                     /locus_tag="ACHKG5_003358"
     CDS             16382..>17625
                     /gene="icd"
                     /locus_tag="ACHKG5_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0869397.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIK"
BASE COUNT         4639 a         4227 c         4603 g         4156 t
ORIGIN      
        1 aagattttat tcttactttt tcattttctg taaatcatac atcctcagta tttgccattc
       61 atcactctag cgggaacaat acgacacaat aagacacctg atgactcttt aagaaacgaa
      121 agggggcaat agtgttaagc actgatcggt ttatacgtga aaaagaatgc gaaaaactaa
      181 ccggccttag ccgtacgtgt cgctaccgcc tggaaaaggc cggacaattc ccatcacgtc
      241 gtaaacttgg cggtcgttcc gttggctggt ctttatccga ggttctggcc tggaaggata
      301 gctgcaaggc agttcattaa tcacgctggc ggcacacagc cgccacacat caatcatctg
      361 aacacagagc tataaccatg aagattgaat atacgccaga acgtgggcgg ggattcgttc
      421 gccctggtga gactggaaaa ccacaaaatt ggggtttttc aggcataaaa aaagcggccc
      481 cgaaatggag ccgcctttct gaacagataa cccgctgcgc cttatgtgta tatgatccca
      541 aacataagca cggggatgat agccgctatc aggctggtgg gcaatgctat cagtctggtt
      601 cagttcgttg ccatacctgc aatgggcgcg tttccctgta ctctttaagg aattgctcaa
      661 gggcaaaagc acatggcgcg aatctttctg attcatgctc tatctttctg cgccgtcttt
      721 tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt cattgtgttg tcctgcatag
      781 caatgcgccg taatacctca caccacggcg ctgatagttt ttatcctttg ggttctatgc
      841 cgcgttgctg taattctttg cggagaacgc gtttaatcca agtggctaaa gatgtgtcgc
      901 catcggcctt agcagcttct tctagctgcg ctctgaagtc ttccggtaat cgcatttggt
      961 atggaggcga acgtttttct aatggtgttg acatggtaat taccctttat tgctatacct
     1021 acatggtaat taccattgta cttgccatta cacaaaaatc aacgccccgc agtgctggga
     1081 acacatacgg ggcgtctaac caacaacgta aactaggagc cgttatggtt gccgtaaatc
     1141 atataccaca ccttgtacac acacaaacgg cctttgtgtg gcgttttctg gcactgagtg
     1201 ccggagaatc tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg cgtaaccgtt
     1261 gcccgtccgg ttgtgttgct gtatttgccg cccgtattcg tcaggaggtg cgccatgatt
     1321 aatctgtcac ttactgacct taaccgcatt cagtttcgtg agaaattcac agggcagcta
     1381 ctggtcaatg tggataaagg tcgcgtggtg tgtaattacc acttaccaga tgaagcaatt
     1441 gtcgccacaa gggaatcatt acaggaaatc acaaaacgtg ccggaatgat taacacgaat
     1501 attcagggga tgctttatgc acaataaaac cacaccggac gcagccgccg aagccatcaa
     1561 aacgctgatg cacgcgctga ttgatatttc tgttatcgcg gacagggcac ataagcacgc
     1621 caccagcgaa acagaatatg ccggtgcttt cgttcctcac tcactggctg tcatgcaatt
     1681 tagtgctgat acggcgctga atgaggccaa agctatcctg attgctgatt gtgaaaatgg
     1741 gggggttatg cgtgatgatc gttttaattc cctgaaacag gaattttccg gcgttcctga
     1801 tgatgcggct gatgcgcttt cgtcaatgcc agaacttatt agagcggctt ttttcttact
     1861 ttccacgaga gaatataaat caacggggct tgatgtactg aatatcgccg ccgattatgc
     1921 ggaatatgtg gcagaggcgc gttatagaag aaaatttcct gaggatgtaa gccatgcgtg
     1981 atatttacca ccaaacaata gaccgcgcat ttcttgcact ttctcacagt gaaaacatga
     2041 tggaaatatt gcgcatatgg cttgaaacac ttggcgacaa tgaacgcgac aaacaaaaat
     2101 caagaattgc cacggcatta ataacgcttc ttgagcctgt aataatggaa ctgcaagaaa
     2161 tagatctatt gcacgacaga tataaagaac agcacaccgg agaataaaaa taatgaaact
     2221 taaatattct ggcttaactg ccagtggcaa cactcaccct aaatttacgc gcggtgatat
     2281 ttaccgcgac cagtacggcg gcacggtaat gattaagggc gtggaagaac ggcgcgtaac
     2341 ctaccgccgt gaaggttacg aatatgattg cgtgatgcct gtttatcagt tccggcgtga
     2401 tttttctctg gtacagaccg cgccgcataa cgtgcccacc agcaacgcca gggcacgggc
     2461 aaacatccag aagctgaaaa ccatggttaa cggattcagg ggcaagaaat gaaaagcgca
     2521 ccgaacttaa aaaaacagcc ttacgacaag atgaccgaag tcattatttt tgcgggtagt
     2581 gatgcctggg cacatgcgaa acagtggcag gaacaggacg ggcgactggc tggcgataat
     2641 gtgcctcccg ttgtgctggc tgatgatcaa ctggatgaac tggcagaact gagaatcatc
     2701 gacgaggggc gctattgtgt ccggctgtac aaggcaggcc acatcaggcc atcaaatatt
     2761 aatgccattg cgcacaagct ggcggcggcg ggtgtaactg atgcgaatta ttaccccgaa
     2821 gggatgcaca gccatatgcg ggagaactgg cgcgaatacc tggaacgggt gcgcgggaaa
     2881 gagccggcgg aagaaaaaaa ccaccagcga aaaaccacgc taccgatgag cgttggatct
     2941 accggatacg acacgcaact ggattacgtg gttaagggga ttattccggc ggtatcgcta
     3001 tgcagcatat acggggctag cgggtcctat aaatcattcc ttgccggatc gtgggcgtgc
     3061 catgttgcca ctggtcgcca gtggggaggc cgcagggttg cacatggtgc ggttctctat
     3121 gtggttggtg aaggcggtat aggtgttccg cgtcgtgtaa aagcctggga ggttgtgcac
     3181 gatgagcagg tgaaaaatct gtatctggta aaccgcccca tctttccggc tgccccgctt
     3241 gatgttgatg aaatggttat cgctgcccgt caggtggagc gggaaacggg taaacctgta
     3301 cgcatgatta ttctggatac gctggcgcgt tgctttggtg ggaatgatga aaatgattcc
     3361 cgtgatatgg gggcgtttat ccgtggttgt gacgaactga aacgacgcac aggggccacg
     3421 gtgctggtgg ttcaccattc cggcaaggat gagacgaaag gcgcgcgcgg ttccagtgca
     3481 tttcgtgctt cgctggatgc tgaataccgg atacgcaggg aggacgcagg aagcgaagcg
     3541 ctggttatct catgcaccaa aatgaaggac gcggaggaac tcaaagaagc cgcatatgac
     3601 ttacgcgtgg tggagctttt taccgacgct gacggtgaat taatcacgtc gctggtggtg
     3661 gtggatgatc cgcgccctcc tgttgaactg gagcgcatcg aggaggcagg gaacaagacg
     3721 gaaaaccata ccgcgctatg ggggtgcatc cgttcacgca cacagaacgg cgacaagtgc
     3781 acgatcccgc tgttacgtga tgacatgaaa aagctggggt atgaaatgaa aaacttccgg
     3841 cgctggctgt acaagctgga aaaagatggg gttattcgta tcgatgggga tgatgtagcg
     3901 ccgctataaa agtgaggagc aaaagcgagg gggatagaaa gagggccaaa attagcccgc
     3961 tctccctcac ttttcgacct gtatacatcc tcaaaagtga ggggtaaaaa aatacttatg
     4021 aaacacacac atagaaaaac cgaaaatccc aactgcgacg aagtgagacg cttgaaaaag
     4081 tgaggcgaaa aagtgagagg ttgcgagaaa tgacccaaaa acgcagagac agaacagagc
     4141 caaaatataa agcgttagac atgactgagc acaccttaaa ggtggcaatc agaacgatag
     4201 accgccacac gcgggaagga tacgcgaagg aacatcccga cctgataagc gcattcatga
     4261 ccacggcggc ggcaaacttt gccacgctga cagaacggga gattgccgaa gcggaacagg
     4321 taacaaccat caacgttaaa accggagagg tggaattatg acggcacaga tagccgctta
     4381 cgggcggctg gtggacgacc cgcaggtaaa acagaccagc aagggcacac cgatgacgct
     4441 ggcgcgtatg gcggtatctt tgccatgcag ccaggcacag gacggacagg cgacgttatg
     4501 gttatcggtc atcgcatttg gcaaacaggc cggcttcctg gctaaacatc aaaaaggcga
     4561 tgttgccagc gtatccggca cgatgcaggt aagccagtgg accggacaga acggagaaac
     4621 gcggcagggc tggcaggtta tcgcagacag cgtaatcagt gcccgcgcgg cacgtccagg
     4681 cgggaacaga cgcaaaacca caggcacaca gggtaatcag ccaccagcgg gaggcgatga
     4741 cccttacggt gatgatattt cgttctgagg gggtgacgat ggtacatgac cgcatagcgg
     4801 aggaactgga ggcgaaaggc ttttaccgga gggcggcggc gcgatggggt gaagtcatgc
     4861 agctggtgga gacagacaag gaacggcatc acatcacgat gcgacggctg gaatgttcaa
     4921 ggaaggcaca gagggcaccg gagccgccgg ataatttcgg agacctgaaa aaggcagtcg
     4981 atcgcactta tgccgaaatg ggtatagatg gtgctggtga tgaaatatgg cgcaattacc
     5041 aggacagcta atcaaacagc cggagaaatc cggctttttt tgcaccagtt gaaacggtat
     5101 ggcgcattac cgggttttcg tcacggtcag gcatagttac tatctgaaac aaacagacac
     5161 aacagaggaa aaaaacaatg ccgatgaaat ttgatgagat attaaaacag cgtgataaat
     5221 accatgctga caacatggag acgatgagca tcaatgatta tcgcgcattc ctggagacgg
     5281 gcgcactgat tgaaaaggat cagcatggtt ttgtgagatg tgctctatcc ggtgaaatgc
     5341 tggcggtaaa tcctgaacag atagatgcat tgatagaatt tctgaaagag atcagagact
     5401 gagccagcac acagcacaca tagccggagc aatccggctt ttttgcgccc aaaaaaagcc
     5461 cgataaggtc agagggttct tatcgggctt ttgcatatga ggtttttgga tgcactggcg
     5521 ttcgtgatcg ggataatcat ttcataattt gcaacataac tcaatatcat tgcataaaat
     5581 gcaattctga ttataatcag gactggataa acatccagtt atgatttttt tagtcgaaga
     5641 ggaatttctt actatggctg aagagaaaaa aagcggtgtt tcggtgtaca taagccccga
     5701 aatcgtggag gtgctcaagc agcgccacaa aaaaaactat gaagctggcg ttgcggctgg
     5761 actggatccg ctgatgacgc cggagccgtc gataggttca cttgtacgct cttatttact
     5821 tgcggcgctt gggatgcata aaaattatgg gggtgaataa tggcaggcaa agcaacggca
     5881 cttaacacta accagctttt catgtacctg aatcgcgggg atattgcgga ttttaaattc
     5941 agccctctgt ttaccacgct gtttttcccg aacgtggcga cattcagcac gcaaaacatc
     6001 atgctggata ccctggacat tgaagaagtc accatgtcgg cgttttgttc gcctatggtg
     6061 ggtagccagg ttcagcgcga taaagggtac gaaaccagca caatcaaacc tggctacatg
     6121 aagccaaagc acgaaatcga tccaacgaaa accatcatgc gcatggctgg agaagatccg
     6181 gcacagctta acgaccctac ctatcgccgt atgcgcctga ttactggcaa catgcgccgc
     6241 cagataaacg ccattaaggc acgcgtggaa tggctggcgg tgaatgcggt aacgaccgga
     6301 aaaaacatca ttgagggcga aggcatagaa cgctatgaaa tcgactggaa aataccggaa
     6361 aactgcatca tagagcaggc caagggtaaa aaatggtccg agcaggataa agacatgcac
     6421 gacccaatct atgacatcga gctttatgct gatcaggctg gttgccccgc aaacgtcatg
     6481 attatgggcg ctgaggtatg gcgcacatta cgcagcttta aaaaattccg tgagctgtac
     6541 gatctttccc gtggttcaga atccgccgcc gaactggcct gtaaaaacct gggcgaagtg
     6601 gtgagcttta aaggctatct tggtgatctg gcccttatcg tctattccgg caaatacact
     6661 gacagcgatg gtaccgaaaa atatttcctt gagcctgatt tgctggtcct gggtaacacc
     6721 aacaataaag ggctggtggc ctatggtgcg ataatggatc aggaagcggt aagaacgggc
     6781 gcaacacaaa acatgtttta cccgaaaaac tggattgagg acggcgatcc ggcgattgag
     6841 tacgtgcaga cacacagtgc accgcagccg gtaccggcag atattcgcaa atttgttacc
     6901 gtcaaaattg gttaacgggg ggattctatg gacactccat acattgagtt atttgcaggc
     6961 agtcagcagg tatccacgac gttggtacat tttgccgctg atgctggcgt tattcaggaa
     7021 tttaccccgc tgatgctggc agacaatggc gagtttaagc cgtgggatgg tcaggaatct
     7081 ggcaaggctg tttatctgac ctcgtaccct gtggacacgt caaagcagaa atcagcacag
     7141 tgttacaaga cggggatatt caatattgcc gccgttaact ggcctgagag cgtcgacacc
     7201 gatgcgaaaa aatgcgccgc ctttgcgggt tccggcgtat ccgttcagcc gctggcccga
     7261 taagcagggg gaacgatggc aacgaatgaa agcatcatga cgctaccgct ggcgagtaaa
     7321 tttaaagtcg aagcgcgggc aatggctgac agaggtttat caacctatga ggccgtatat
     7381 caactaaaca aactggaaga gcaggacaag ccgtgcgctg atgcgattat ggcgcttcat
     7441 gagcataaag actatcagcc gctgttgcgt gcaatggcaa acgtgccttg tatcgatgtt
     7501 ggtacggcta aaagcatcct tagcatgacc atagagcagg aacgtccgaa ggttgcgccg
     7561 gaacttaccg cagcctttga aaactttatg gacatgcaca gcccgcaagc cgtatcagct
     7621 ggcatggcat acgatggcag aaaccagggc gatgacggcg acatcgatcg catactgaaa
     7681 accatctgag acaaggccgg agaaatccgg cttttttacg ggtccttcct ggaattatgg
     7741 cccgttacgg ggcggcgacc tcgcggattt tcgctattta tgagcctttt cagggtggtg
     7801 gtggtggttt tgttgtttga tctatcttta tgaatgaaaa gggaaagatg caagcaatac
     7861 accaacctga agcagtaatt aagttggtgt attaatgaaa tctcatctga tgaacaaaaa
     7921 aaacatggcg aaaagctgcc gtgtaagtgc gacagcgttc gacaagtggg gagtaactcc
     7981 cgttgagcgt aaaggccgtg atgcgtttta tgatgttgcc agcgtgatag aaaatagggt
     8041 taacaatgca attagccagc ttataaacga caaaggcgag attgacgatg atgaactttt
     8101 acgagtcagg atcagattac tgacagcaca ggcagaggcg caggaactta aaaacgatcg
     8161 cgatcgcggt gacgtgattg atactgagtt ttgcctgtac gcgctttcaa agctggcgag
     8221 tcagatttca tcaatcatgg acagcctccc gcttactatg caaaggagct tcccacagat
     8281 tacccccgcc atgctggaca ggcttaaaag ggaagtggtt aaagcctgta atgccagtgc
     8341 cagagttgcc gacaacctcc cacagatact ggctgattac ttgaaagaaa caaccggaaa
     8401 cgtaccggaa aagttgctac agaagaaagg cgagtaacag acgcgcaatt attgaacaaa
     8461 aatgagaaag gatctgataa tggcattttc tggctaaaat gcctcgtgtc agatcctttc
     8521 tttttttaat attttcattg ttaaacagtc tgttacagag aagaagatcg gatctgactt
     8581 ttgccggaaa attctcataa acagataaaa accgcgaggt cgccgccccg taacgggcca
     8641 taattccagg aaggacccga cgacaccaga ctatcagagc gatgggggca caatgacaga
     8701 agccgaacta ctgggattaa tccgccgcgt cgccggaatc agccagcagc atgacgaaca
     8761 ggccacacag ccggacagcg tgaccgctga aaattatgtg cgtgttgtag cggaagtgat
     8821 gcgccgtgat ggtatagaac ttaatggcgt tgatatgcgc aacatacgga accgcgttct
     8881 tgaaatgctg gcctacaatc gccgcgttga actgtatcgg gagaaagaaa aaataacgta
     8941 ccactggaag aagccggaac ggctgcggcg gtaacttgct gatattcccg ataacgcaaa
     9001 attgcgctgg ctacatcctc acatctgagg actgttaatc agaaggccgg aatatattca
     9061 caaactacga aattttcgta gtttaattaa tggtcagatt aatcgctaac ccgctgatat
     9121 tttcggaaac ctcaatttga ggaagtcgga agcgtcaccc ttcatattct gaatggtcat
     9181 aatgaccacg cagaccatca aggcaaccac aacgaacatc gttacggttg gagcttttac
     9241 cccactgggg gaaaggtatt acgataatca taacacctac cgaacaggta aaacccacca
     9301 gcctgattaa cagttaaccg gaaaaaaaat ccggtagccc cgttatgggg atccccatat
     9361 cgacattaac gcccctcatg aattggtgcg cttccccctg gaaagattac ccgcctgtat
     9421 atttcttgtg tctatttgtt ccacgttgtt tcatacagtg caccgaacgg tgtagttaat
     9481 ggtgtagtca atttgctgtt tttagcactt tttgcagtgt gggttgttta attaaatcaa
     9541 tgggttgcct tgctgtggtg ataaaaggac atatttattc atctttcggc gtagaatgct
     9601 ggcgaccaaa aatcacctcc atccgcgcac cgccaagcat gctctctccg gcgacgattt
     9661 taccctcata ttgcttggtg atttcgcggg ctaccgccag ccccacacct tgcccagggc
     9721 gtaaagtatc aacccgttga ccacggtcga aaatgacctc tcgcttgctt aatggaatac
     9781 cggggccatc atcctcgacc acaatataga gatgctcgtc ggtttgcctt gcagaaattt
     9841 cgacaaactc gaggcaatat ttacaggcat tatccagtac attgcccatc acctcgacaa
     9901 aatcgttctg ctcaccgaca aagctgatct ctggcgaaat atcgagagag atattgaccc
     9961 ctttgcgttg atacactttg ttcagcgccg aggtgagatt gtccagcagt ggggcgaccg
    10021 gatgcagctc gcggctgagc aatgtcccgc cgtgcatact ggcacgatgc aggtagtagc
    10081 caatttgctg tgaaatgcgg ctgatttgct ccagcattac cggttcagca tcactgacgc
    10141 tcatcttttc actacgtaga gaacgcagcg tactttgcag caccgccagt ggcgttttca
    10201 gactatgggt caggtcggtg agcgttgtac gatatttgtc gtaacgttcg cgttcacttt
    10261 ttaacaatcg gttcaggttt cgtaccagac tggtcagttc tcgcgttgtg gctggattga
    10321 gcaattcgcg gttatgttct tccagttcgc ggacttcttt tgccagggct tcgatggggc
    10381 gtaaactcca ccaggcggcg acccacagca gcgggatcac taacagcaga ttggctgaga
    10441 gcacatagat aaaccagctc cagaccatat aggaactttt tagctccacc ggaatggtat
    10501 ccaccaccac aatggttaac tttggcatcc gcgatgttgc cgggtagacg tttaccgcca
    10561 ccgagtgggt catctccgcg tcgtcatcat cttcccgcac ttcctgcaac tgttgctgta
    10621 tcgaatgatc tccactcagc aagaggctgg tatcgttaac atccgcttca atttcatgaa
    10681 aaccattcga tttcagccag tcaggctgga tcatcttcat cagccagggc acgtcacgtt
    10741 gcgcccataa aagctgcccg ttctcatcat aaattagcgt catggtgggg ctttgcttgt
    10801 cgatattttc gggtaactcg acatgcaact tattgttttc ccacttcgca agggtataga
    10861 acagattgct ctcgccacgt aacagccgaa acgtagtttt atcgaaactg acgctataac
    10921 cgatcagcgc gaccattccg taggcaagcg aaagcaccag tactaccgct gccgttgcca
    10981 acagaaaacg tacccgcagc gagagcggga aaaaaagacg cagtaatttt ttcatcagcg
    11041 caattcgaac aaatagccct ggccgcgaac ggtagtaatc acttcttgcg gatattgtgc
    11101 ctgaattttt ttgcgcagac gtcccatcag tacatcaatg gtatggcttt cccgcagctc
    11161 cgcatccgga tagagttgta gcattaacga atctttgctg accactttgc cattattgcg
    11221 tatcaacgtt tccataatgg tgtattcgaa cgcggtcagt ttgatcactt cgtcattaat
    11281 agataattca cggcgagaga gatcaacctg aaacgggggg agcgaaatga cctgtgaagc
    11341 caggccgcta ttacgccgca ttaatgcctg cattcgcgcc atcacctctt caatatgaaa
    11401 cggtttagtc acgtaatcat cagcaccggc acttaatact tcgactttgt cctgccagct
    11461 ttcacgggcg gttaatacca gaatcggcag tgaaacatca ttgctacgcc agcggcaaat
    11521 cagtgacaga ccgtcctcgt ctggcaatcc gagatcgaca atcgcaatat ccggtaaatg
    11581 ttcattgaga taataatcgg cttctttggc atcttctgca tcatcgacct gatgaccagc
    11641 atcctgaatc tgaactttaa ggtggtgacg taacaacgca ttgtcttcaa caaccagtac
    11701 gcgcattttt atttctccct gtcttaatta ttaaaatagt gtaacgcgat tatgtggtta
    11761 tgggggtaaa cattaaataa accagcgggg gaggggaggt aaagtgaaaa aataaaaagc
    11821 ggataatctt aataagcagg ccggacagca tcgccatccg gcactggtac gagtttattt
    11881 cagctcatca accatggtga tggcgcgacc aatgtagtta gccggcgtca tcgctttcag
    11941 gcgggctttc tcttcttctg gcaacgccag accgtcgata aactgcttca tgccttcggc
    12001 gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg tacggttttt cgatgccata
    12061 gcgacgcata actgtctgga ttggttctgc cagcacttcc cagttgtgat ccagctcatc
    12121 cagcagatgg tcacggttca cttccagttt gctcacgcct ttcagggtgg attgatacgc
    12181 aatcagcgca taaccgatac ccacgccgag gttacgcagc acggtggagt cggtcaggtc
    12241 acgctgccag cgggaaaccg gcagtttgct tgccagatgc tgcaataccg cgttggaaag
    12301 gcccagattc ccttcggagt tttcgaagtc gatcgggtta actttatgcg gcatggtgga
    12361 agaaccaatc tcaccagcaa tggttttctg tttgaagtgg ttaagggcga tataacccca
    12421 gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat caaacagttc
    12481 ggcaatgtag tcgtgcggtt caatctgggt agtgtacggg ttccattgaa tacccagcga
    12541 ggtgacgaac tcttcgctga actgatgcca gtcaacttcc gggtaagcgg cgatgtgggc
    12601 gttatagtta ccgaccgcgc cgttgatttt gccgaggatc tccacctggt taagctggcg
    12661 gtactggcgc tccatacggt aggcgacgtt tgccatctct ttaccgatgg ttgacggcgt
    12721 ggctggctga ccgtgggtac gggacagcag cgggatatcg cgatactgaa cggcgagatc
    12781 tttaatgcca tcaattagtt gacgccagta cggcaggatc acttcatcac gcgcggtttt
    12841 cagcattaat gcgtgggaga ggttattgat atcttccgaa gtacaggcaa agtggatgaa
    12901 ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt tctttcagga aatactcaac
    12961 cgctttaacg tcgtggttag tagtacgctc gatggttttg atgcgtgcgg catcttcttc
    13021 gctgaaattg gcgacaattg catcaaggta accgattgcg tcggcagcaa aagcaggaac
    13081 ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa cttgtacacg
    13141 gaatttcagc aaaccatatt cgctgaaaat cccgcgcagc gcgctgactt tatcgccgta
    13201 gcgtccatcg acaggggaaa cggcggtcag tgaggataat tccatagatc acaactccgg
    13261 ggttaaatga gcaagaattt gttttgcctg agtggtcagg cgattacgag aaaacatcag
    13321 ttgcagacgt ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag
    13381 ggttgcgcga actttcgcct gcacttgtgg gctttgcagt acagcagggg aaccggtgac
    13441 ctgaatgcgc gggccaagcg ggctgatcac atcaacatag atagcagcca tcgcgctcat
    13501 cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt
    13561 gcccagagtg tcgagcgcac ctttcgctga ggagagtttg cgcttaagca ccatcaggct
    13621 gagtgtgtag cgggttaatt cggcgtttaa gccctggcga ctgctggcat tgagcacgcc
    13681 gagcagggtt tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt
    13741 cgagctgggg ttcatatcaa taatactgtt gagtgagacg tgtagcgcat cggcatcaca
    13801 atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac cggccagggc
    13861 gagggtgatg tcatagtaat tctttgccac gttcactgct tccttgtttt aagtaaagat
    13921 aataatcaga tcggcagcgg cagacgctgc tcaataatac cgccaccgag gcacacttca
    13981 ccgttataga agacggcaga ctggcccggc gtcacggcgg caacgggctc atcgaaaatc
    14041 acttcaatgc gatcatcgtc cagcgccttg acggtgcagg ggatatcggt ctggcgatag
    14101 cgggttttta ccgtgcaacg catagtaccg gtgaatggtt cacgatcgac ccagtgcagc
    14161 tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt catggccctg cgcaacaatc
    14221 agaatgttgt tttcgacgtc tttatcgacc acataccacg gttcttcggt gccttctttg
    14281 gtgccaccga tacccagacc tttgcgctga ccgagggtgt gatacatcag cccctgatgc
    14341 tcaccaattt catcgccatc gactgtaatg attttgcccg gttgcgccgg gagataacgg
    14401 ccaaggaact cgcggaattt acgttcgcca atgaagcaaa tgccggtaga gtcttttttc
    14461 ttcgcggtga ccagaccaag atcttcagca atcttacgca cctgcggttt ttccagttcg
    14521 ccgaccggga acaggctttg cgcaatctgc tcatggctga gcgtataaag gaagtagctc
    14581 tggtctttat tgctgtccag accacgcagc aggcggctct tgccatcgac atctgcccga
    14641 cgaacgtaat gaccggtagc gatataatcg gcacctaaat cttcggcggc aaattcgagg
    14701 aaggctttaa atttgatctc tttgttgcac agaatatccg gattcggcgt gcgaccggct
    14761 ttatattcgg caaggaacag ttcgaagacg ttgtcccagt actcggcagc aaagttaacg
    14821 gtgtgcagtt caatgccgag cttgtcgcag acagcctggg catcagccag atccgctgcc
    14881 gctgtgcaat attcctcacc gtcgtcttct tcccagttct tcataaacag gccttcgacc
    14941 tgatatccct gttgttgcaa cagccaggca gaaacggagg aatcgacacc gccggacatg
    15001 ccgacgatta ctttttttgc ggtttcagac attggatcac tcacgacatt gaacttcaag
    15061 gcggcgtatt ctatcacgca gccttttaga tgacaccctt tgtaaaaggc cagttaaaat
    15121 cgccaatcat ctccagcgga tagcgttgcc cgctttgata acaacggata ctttccgcca
    15181 ccagcggcga acgaagattt gacgcctgta aaatttcttc ggcgctgacc caacgacagc
    15241 agtcgatatc gctgtcatga ggctgggtcg ggcatatttg ctcaagctca atggcaaaga
    15301 ggaaacgcaa aaacggcgtt ttatctggcg caatccactg atgcatacga ataaagtgtt
    15361 gcggctgcgc gctgatgccg gtttcttccc acagctcacg ggcggctgct tccactaagg
    15421 tttcatcggc ttccagatgc ccggcaggtt ggttccataa ggctttacca ttaatcgtct
    15481 cttcaacgac taaaaatttc ccctctgcgt gcactacgca agcaacggta acgtgcggtt
    15541 taaacattgt gcatccttat tctgttgctt ctcgccattc gccattggca aggttatcca
    15601 aagagtaatc acccatcgca tagcgaatca gtcgcagcgt ggggaaacca acatgggcgg
    15661 tcatgcggcg cacctggcga ttacgtcctt catataaggt gatcttcagc cagctggtgg
    15721 gaatactttt gcgttcacga attggtggat tccgcggcca taaccacgcg ggttcgtcaa
    15781 ccagctccgc gcctgcgggc agggtagggc catcatttaa ggttacgcca ttgcgcaagg
    15841 cttcaagtgc gtcttgtgtg ggaatacctt ccacctgcac ataatagatt tttccggtgc
    15901 gtttgcccgg ctgggttaaa cgcgcctgca acgcgccgtt attggtcagc accagtaacc
    15961 cttcgctatc gcggtcaaga cgacctgctg cataaacgcc ctgaaccggg atgaattctt
    16021 ttaatgtttt gcgtccggct tcatcggtga actgcggaag aacatcgtag ggtttattga
    16081 acaggatcac acgcgtgggc tggttttcag gtttacgcct ggtagaacgt tgtgagctga
    16141 atcgcttaac ctggtgattt ctaaaagaag ttttttgcat ggtattttca gagattatga
    16201 attgccgcat tatagcctaa taacgcgcat ctttcatgac ggcaaacaat agggtagtat
    16261 tgacaagcca attacaaatc attaacaaaa aattgctcta aagcatccgt atcgcaggac
    16321 gcaaacgcat atgcaacgtg gtggcagacg agcaaaccag tagcgctcga aggagaggtg
    16381 aatggaaagt aaagtagttg ttccggcaca aggcaagaag atcaccctgc aaaacggcaa
    16441 actcaacgtt cctgaaaatc cgattatccc ttacattgaa ggtgatggaa tcggtgtaga
    16501 tgtaacccca gccatgctga aagtggtcga cgctgcagtc gagaaagcct ataaaggcga
    16561 gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa tccacacagg tttatggtca
    16621 ggacgtctgg ctgcctgctg aaacccttga tctgattcgt gaatatcgcg ttgccattaa
    16681 aggcccgctg accactccgg ttggtggcgg tattcgctct ctgaacgttg ccctgcgcca
    16741 ggaactggat ctctacatct gcctgcgtcc ggtacgttac tatcagggca ctccaagccc
    16801 ggttaaacac cctgaactga ccgatatggt tatcttccgt gaaaactcgg aagacattta
    16861 tgcgggtatc gaatggaaag ctgactctgc cgacgccgag aaagtgatta aattcctgcg
    16921 tgaagagatg ggcgtgaaga aaattcgctt cccggaacat tgcggtatcg gtattaagcc
    16981 gtgttctgaa gaaggcacca aacgtctggt tcgtgcagcg atcgaatacg caattgctaa
    17041 cgatcgtgac tctgtgactc tggtgcacaa aggcaacatc atgaagttca ccgaaggcgc
    17101 gtttaaagac tggggctacg agctggcgcg tgaagagttt ggcggtgaac tgatcgacgg
    17161 cggcccgtgg ctgaaagtta aaaacccgaa caccggcaaa gagatcgtca ttaaagacgt
    17221 gattgctgat gcattcctgc aacagatcct gctgcgtccg gctgaatatg atgttatcgc
    17281 ctgtatgaac ctgaacggtg actacatttc tgacgccctg gcagcgcagg ttggcggtat
    17341 cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg
    17401 tactgcgccg aaatatgccg gtcaggacaa agttaaccca ggttctatta ttctctctgc
    17461 tgagatgatg ttacgccata tgggttggac tgaagcggct gacctgattg ttaaaggtat
    17521 ggaaggcgca atcaatgcca agaccgtaac ctatgacttc gaacgtctga tggaaggcgc
    17581 taaactgctg aaatgttcag agtttggtga cgcgatcatc aagaa
//
LOCUS       DAZGXS010000097        17556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGXS010000097 DAZGXS010000000
VERSION     DAZGXS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            74..1123
                     /gene="allD"
                     /locus_tag="ACHKG5_003359"
                     /pseudo
     CDS             74..1123
                     /gene="allD"
                     /locus_tag="ACHKG5_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
     gene            1145..2380
                     /gene="allC"
                     /locus_tag="ACHKG5_003360"
     CDS             1145..2380
                     /gene="allC"
                     /locus_tag="ACHKG5_003360"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0869398.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2391..3176
                     /gene="allE"
                     /locus_tag="ACHKG5_003361"
     CDS             2391..3176
                     /gene="allE"
                     /locus_tag="ACHKG5_003361"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0869399.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3404..4549)
                     /gene="glxK"
                     /locus_tag="ACHKG5_003362"
     CDS             complement(3404..4549)
                     /gene="glxK"
                     /locus_tag="ACHKG5_003362"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0869400.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4571..5872)
                     /gene="ybbY"
                     /locus_tag="ACHKG5_003363"
     CDS             complement(4571..5872)
                     /gene="ybbY"
                     /locus_tag="ACHKG5_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0869401.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5929..7290)
                     /gene="allB"
                     /locus_tag="ACHKG5_003364"
                     /pseudo
     CDS             complement(5929..7290)
                     /gene="allB"
                     /locus_tag="ACHKG5_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(7327..8718)
                     /gene="ybbW"
                     /locus_tag="ACHKG5_003365"
     CDS             complement(7327..8718)
                     /gene="ybbW"
                     /locus_tag="ACHKG5_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0869402.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(8887..9765)
                     /gene="glxR"
                     /locus_tag="ACHKG5_003366"
     CDS             complement(8887..9765)
                     /gene="glxR"
                     /locus_tag="ACHKG5_003366"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0869403.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9866..10642)
                     /gene="hyi"
                     /locus_tag="ACHKG5_003367"
     CDS             complement(9866..10642)
                     /gene="hyi"
                     /locus_tag="ACHKG5_003367"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP0869404.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10655..12436)
                     /gene="gcl"
                     /locus_tag="ACHKG5_003368"
     CDS             complement(10655..12436)
                     /gene="gcl"
                     /locus_tag="ACHKG5_003368"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP0869405.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(12526..13341)
                     /gene="allR"
                     /locus_tag="ACHKG5_003369"
     CDS             complement(12526..13341)
                     /gene="allR"
                     /locus_tag="ACHKG5_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0869406.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13419..13901)
                     /gene="allA"
                     /locus_tag="ACHKG5_003370"
     CDS             complement(13419..13901)
                     /gene="allA"
                     /locus_tag="ACHKG5_003370"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0869407.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14131..15057
                     /gene="allS"
                     /locus_tag="ACHKG5_003371"
     CDS             14131..15057
                     /gene="allS"
                     /locus_tag="ACHKG5_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP0869408.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15126..16220
                     /gene="mnmH"
                     /locus_tag="ACHKG5_003372"
     CDS             15126..16220
                     /gene="mnmH"
                     /locus_tag="ACHKG5_003372"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0869409.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            16336..16701
                     /gene="ylbG"
                     /locus_tag="ACHKG5_003373"
                     /pseudo
     CDS             16336..16701
                     /gene="ylbG"
                     /locus_tag="ACHKG5_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17451..>17556)
                     /locus_tag="ACHKG5_003374"
     CDS             complement(17451..>17556)
                     /locus_tag="ACHKG5_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869410.1"
                     /translation="DGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP"
BASE COUNT         4605 a         4604 c         4178 g         4169 t
ORIGIN      
        1 tcacataatc cagatatttt ccctgtatgt taattgcagt catgcttcac accgtcgtta
       61 aaaaggaaga cagatgaaaa tcagtcggga aacactccac cagctaattg agaataaact
      121 ctgccaggct gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat tggtttatgc
      181 cgatgccaga gggatccact ctcatggcgc ggtgcgcgtg gaatactacg cggaacgcat
      241 ttcaaaaggc ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg ggccgtgctc
      301 ggcaatttta catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca
      361 tgccatcaaa accgcccagc aaaatggcgt tgcggtggtc ggtatcagcc ggatgggtca
      421 cagcggcgca atctcttatt ttgtgcagca ggcagcccgc gccggattaa ttggcatttc
      481 gatgtgccag tccgatccga tggtggtgcc gtttggcggc gcggaaattt actacggtac
      541 taacccactg gcctttgccg cgccgggaga aggcgacgag atccttacct ttgatatggc
      601 gactaccgta caggcatggg gaaaagtcct cgacgcccgc tcgcgtaata tgtctatccc
      661 ggatacctag gcggtcgata aaaacggtgc accaacaacc gatccgttcg cggtacatgc
      721 tctgctcccc gcagccgggc cgaaagggta tggcctgatg atgatgattg acgtcctctc
      781 aggcgtctta ctcggcttac cgttcgggcg acaggttagt tcgatgtatg acgatttaca
      841 cgccgggcgt aatttggggc aattacatgt agttattaac ccgaactttt tctcctccag
      901 cgaattattc cgtcaacatc ttagccagac catgcgcgaa ttaaatgcca ttacccccgc
      961 gcccggtttt aatcaggttt attatcccgg acaggatcag gatattaaac aacgccaagc
     1021 cgccgtcgaa ggcatcgaaa ttgttgatga tatttaccag tatttaattt ccgacgcgct
     1081 ttataacacg tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga gacaggactt
     1141 tcttatgatt acacattttc gtcaaaccat agaagaagcg ctgccctggc tttcctcttt
     1201 tggcgctgac ccagcgggtg ggatgacccg tttactttat tcgccggaat ggctggaaac
     1261 ccagcagcaa ttaaaaaaaa gaatggccgc aagtgggctg gaaacacgtt tcgatgaagt
     1321 ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtga ttctgagcgg
     1381 ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg gcgcgctggc
     1441 ggcgcggctg gcaattgact ggctgaaaac tcaatacggc gcaccgctgc gtacggttga
     1501 agtggtggcg atggcggaag aagaaggtag ccgcttcccg tatgttttct ggggcagtaa
     1561 aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa
     1621 tagttttgtc gatgcgatga aggcttgcgg atttactctg ccggacgccc cgctaactcc
     1681 gcgtcaggat attaaagcct ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag
     1741 taatgggcaa tcaattggcg tggtgaatgc aattgtcggg caacgtcgct atacggtgac
     1801 gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt
     1861 ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga tgggcgatcc
     1921 gctggttctg acctttggga aagtagagcc gcgcccgaat acggtgaatg tggtgccggg
     1981 taaaaccacg ttcaccattg attgtcgtca taccgacgcc gccgtgctgc gtgatttcac
     2041 ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattc gtattgatat
     2101 cgatttatgg atggacgaag aacccgtgcc gatgaataag gacctggtcg ccaccctgac
     2161 agaattgtgt gaaagtgaaa aactgaatta ccgggtgatg cacagtggtg ccgggcacga
     2221 cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca gcatcaacgg
     2281 gatcagccat aacccggcgg aacgcaccaa tattaccgac cttgccgaag gggtcaaaac
     2341 gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa atgggatatt
     2401 taaataacgt caccggttac cgcgatgatt tactggctaa ccgtgcgatt gtgaaacacg
     2461 gtaatttcgc actgttaaca ccagacggtc tggtaaaaaa tattattccg ggctttgaaa
     2521 attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt gattatctgg
     2581 tcacactgca tcaaaacggc ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt
     2641 tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca ttttccttaa
     2701 gcgaaggtgg ctatctttat tgcccgcctg gctccttaat gacgtttgtt aacgcccagg
     2761 ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa ggctatgcac
     2821 cgtggctggt ttctggcaat gccagcgaac tggaacgcat tcactatgaa ggtatggatg
     2881 atgtgatcct gctggatttc ctgcccaaag agttaggttt tgatatgaac atgcatatcc
     2941 tttcttttgc accaggtgcc agccacggtt atatcgaaac acatgttcag gaacacggtg
     3001 cctatattct ttccggtcag ggggtttata acctcgacaa taactggatc ccggtgaaaa
     3061 aaggcgatta catctttatg ggcgcttatt ctttacaggc tggttatggc gtagggcgtg
     3121 gtgaagcgtt cagctatatt tactcgaaag attgtaaccg cgacgtagag atttaagtca
     3181 taccactcgc ctgattattt ttagctatgt tggattttgc cacggtttat accgtggctt
     3241 tttttgcaac atagcctgat gcgcttcgct tatcaggcct acatgatctc tgcaatatat
     3301 agaatttgcg cgattttcag gaaggatatg gtactcatgc cgcatccggc atgagtactg
     3361 cactcttatg cgatataaat cgcatccgct ttaaaggtaa gggtcagttt ttaattccct
     3421 gacctatttt aatggcgcag gcaatatttc gcgcgctgtt gaagagattg gtttcaccgc
     3481 tggcgagcac ttcggctaaa ggtgccagac gtggcaaaat gctgaatacc gcatcaatgc
     3541 cgtactggtg caccacttcc acgccatcac ccaatacgcc agcaatccca atcaccggta
     3601 cattaaactg cttcgccacc gacgcaacgc ccagcggcgc tttaccgcct gccgtttgcg
     3661 agtcaatgcg cccttccccg gtgatcacca gtgctgcccc ctgcaccgcc tgctccaggt
     3721 tgaccgcccg cagtacaatc tcaatacccg gtttaatatc cgcattgagg aataccgccg
     3781 ccgcaatccc cataccgccc gcagcgccac cgcccgccat ctggcagaca ttaatttcag
     3841 tttgctgttg taaaacacgg gcgtaatttt gcagcccctg ttcaagttct tcgaccatct
     3901 ccggcgttgc ccctttttgc gggccaaaca ccgccgccgc gccgcgtgcc cctaccagcg
     3961 ggttatcaac gtcacatgct acttcaatat ggcagttcgc cagacgcgga tcgcattcct
     4021 ccatctcaat gcttgccacg cgcgctaaat tgccgccgtt agccaccagc acctgaccgt
     4081 cggcatcaag gaaacgcaca ccgagcgcct gcgccatgcc cataccgccg tcgaccgtcg
     4141 cactgccgcc aatgccgaga ataatatggc gaatgccgtt atccagcgca tgacgaatta
     4201 actcccccgt accaaaactg gaggccagca acggattacg cttttcaggc gcgaccagca
     4261 tcaggccact tgctgccgcc atctcaatca ccgccgtttt cccgtcgccg gtaatgccat
     4321 aaaaagcatt cactttttcg cccatcggcc cgcagacttc aagcgtcacg atgttgccgc
     4381 ccgtcgcggc gaccatcgca tccaccgtcc cttcgccgcc atccgctatc ggcaaacaga
     4441 tatagttcgc atcgggaaag agggtcgaaa acccggcttt aattgcctga caacattttt
     4501 ctgcactcaa gctctcttta aaagagtctg gcgcaatgac aatcttcatt atcaatcctt
     4561 tttcaacaaa ttattcgatg cgttcccacg gaataaggtt atccataaga acagccagta
     4621 aaatcccgac caataagccg ttactgagca gaggacgaag cgttaatgga aggtcttgca
     4681 gatacacagg cggtaatgcc attaaaaata tgccgacaaa taacggcaat gcgagtcgat
     4741 aaatattgcg agcggtaaac gttatttgct ggctaaacac cagcgcggaa aagagtaaag
     4801 gtagataaga aaccagcatg accgcactac tcacgggtaa agggatactg caaaacagtc
     4861 gcgtgagtgc cggaaccagc gccaccagca ggcaaataac gctgccataa ataaacgaac
     4921 gccgcgtgta atcgccagtc tgggttaata aaccaatgga tgaaacaaac ggtgaaaatg
     4981 gaattactgc cagcggcacg gttatcagtg tcataaatcc ggtcgccaca aagctacgac
     5041 gataacgcgt attccccgcg ccctgctgcg gataaaaaac atccgtgccc cgaatcgcac
     5101 cgtaggtatt gctgatattc accagacctg taatcaccgc cgtcagaata atccccggcg
     5161 acaaagcacc gccactgccg agcgggaacc actgccagtg caactcaccg gagagcgaat
     5221 gcgaagaagg aaagcaaaag taccacaaca accagccggt tatggtgccg accagcaggc
     5281 cataacgggc aaaacgttgc ggcagaaaga taatcatcgc cagtaccagg cacatcaccg
     5341 ccaccgagag cgcgaacggc ggtaactgaa ttttaaaatt cgggtcggct atgccaaacg
     5401 gcagcccgag catacctttg aaaaagatag tggtcagctg cgcgcccagc atcaacataa
     5461 acaagaccat caccgacggc gtaaataacc gcgccaggcg atgacctaat ccgctaaaac
     5521 caatcaacat cgtcagcacg ccggagagtg caatccccac cgccaggctg gtagcgatat
     5581 cgttgatcgg tgtcccgcgc gatgcttcgc caagggtgat agttaggatg gttccccacc
     5641 acaggccccc cggcccttcc ataatagcgc gacgatgacc gcaaaacgcc tgagcgaagc
     5701 aggccagtgc ggtagcaaga aaagcatatt gcgtgagcgt aagcaggcta ctttgcggca
     5761 actgaaaagc agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa ataaagaaaa
     5821 accactgaaa tcctgataac aggctttcgc ggctaactgc aaaattgaac ataaacacac
     5881 cttaaccgga gaatgcccgc cgcaaggacg ggcattgcag gggccagatt actgctgatg
     5941 tttaaggata aattgacctt tcggcgcaac agggaagccc tgttcgatgt cgtaaatcac
     6001 atcaccacgt aagatggttt tcgtgatacg cgcgccaatg gtacggccaa catacgggct
     6061 gactttgtgg cgatattcca gatcgtcatt ggtaagaaca tagctgctat tcggctgaat
     6121 gaagacgaag tcggcatctt ttcctggggc gatacggcct ttttgctgca gaccaaaaat
     6181 atctgctgcg ttagtcgcca ttaatttgcc gaacattggc agagacattc cgcgtttctg
     6241 taccgcttca tcgaacatca cgtccataca gttttgcaga ccggcaatac cgccccatgc
     6301 ttccatgatg ttgccggctt tcatttccgg agggcatggt gagtggtcgg aaaccaggcg
     6361 gtcgatttca ccgttaaaca gtttttccca catgcctttc tagttttcca gatcgcggat
     6421 cggcggtgaa cacttcgcca gagtaccgat ttcttcgaac tgatcggtat ccagtacgaa
     6481 gtaatgcggg caggattcac aagtaacgtc ctgaccttcc tgacgtgcac gagtcacttc
     6541 ttcaacgcct tccgggctgc tgatatggca aacgtgcaga cggcaaccgg caactttcgc
     6601 cagatacagc acgcggcgaa tcgcttccac ttcggtaaat accggacgcg aagccacata
     6661 gtcatgggca gttacgcgac cttcacgttt cgcttcttcg cccagtgcgt cacagatcag
     6721 cgcgttttcg cagtgcacca gcaccggctg ccccagttcg cccagcttct gcgcaccttt
     6781 gaagaactgc cagtcgttta catcacggaa gtcgttgtcg ataccgcgat cgccacaggt
     6841 cgcaacgaag catttgaagc cgacaacgcc cacttcatcc agctcatgca gacggtcgat
     6901 gttgtaagac accaggccac cgagttgtgc cgcatcgata gtcagcttgc ctttagcggc
     6961 atcgaacttc agttcaattg aagcgcggtc aaccgttgca ggcagctggt tgagcggcat
     7021 ttcgatcatg gtggtgatac caccttttgc cgctgcgcga gtaccggttt cataaccttc
     7081 ccagtggcta cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc ccggcgaaac
     7141 caccagacca gacgcatcca taacttcttt tgcatcgccc agatcctgac cgatagcagc
     7201 aattttgccg cctttaacgg cgatatctac aacgcgagct tcgttttcta aaataacggt
     7261 gccgttttta atgattaaat caaaagacat aacaaactcc ttgtaaaata cagattggtt
     7321 tttaactcaa gcaaaggcga cgatgacgcc gacaaaccat gaaacacgcg ataacggttc
     7381 cattaaggga ataaacttac cgccaagaga taaaataacg gccaccagag ttactgaaaa
     7441 cgcggtgagg ttaaaaccgt tatcgtaata tttataatcg ccaggtgcgg tgtacagttc
     7501 atcaagatta atttgcccac gcatcaccac aaaataatgc gccatcatga caccaattac
     7561 cggaccaagc attccgccga taatatcgag gaacagataa atactgtcct gattttccat
     7621 taatttccac gggcagatca gcaggctgat aatactggca atcagtacgc cgtttttata
     7681 ggtcagtttt gtcggtgcaa tggcagcaat ctgataacca gccggaataa tattaccagt
     7741 ggcgttagtg gagatagtcg tcatcagaat aaccagtacc gcaaagaacg aggcaaacag
     7801 gctgtcccaa cgctgaacaa tatccagcac gttccaggta tcagcgccgt aatgaatact
     7861 ggctccggca ataatacata cccccgcgac cgcaaacaga atataggcca caactaaacc
     7921 cagcgtttgc cccagcgctt gctcacgaaa cgagtgggcg ttttgcgtaa agtcggatgc
     7981 gctcaccgcc ggtgccgccc agaccgcaac taccgcgtta atcaccacca ggaacaagaa
     8041 tccactgttt tctgctttct gaataccgct cggaatgtag tcaaagattg gaccgagccc
     8101 gaccagcgaa atcgcccaaa tcgccatacc gccgaaaacg atatagatgc acgggttaag
     8161 aatggcagtg aatttattta aaactttgcc accgccaaaa cctataccaa cgttgaccag
     8221 ccagaagagt aagaaagtaa ttaagcccgg tagagaaagg cctaacagag tgaaatcacc
     8281 accgagagtc aaaaatcccg gccagatttt gccaatcaga atcaagcagg ccagtgaccc
     8341 cgcgtaacat tgcaggccaa accacataat ggcagcaata ccgcctctta ataatccggg
     8401 aaacagtgcg ccacgtacgc cgtaagaagc acgcaggatc atggcaaacg gtacgccgta
     8461 tttactgccc gcagcgccgt ttaataccat taccgcggca atgaaaaagg cgctgaggat
     8521 aattgccagc ataatactaa aggtagacaa gccgagaata aaaaagccgc cgaccatcac
     8581 ataattggga acgttatgaa ccgaacccat ccataaggta aaatagttaa atgttttcca
     8641 ggtccgctgg ctttgcgttt tcggcaatag atcttcgcta tagccgcgtt gctggaatag
     8701 ttttctctga tgttccataa atccctctta atttcacgta tcgaataacg ttgttttaat
     8761 ctagctatga ggaaaaaata aaataaaaat attattttta atactgaagc aacatctttg
     8821 gctgctttct tttttaatat tgctggccga ttcatttttt gatatcggcc atttttattg
     8881 cgggtatcag gccagtttat ggttagccat taattccagc gcctgcacta acgcagagtg
     8941 atccaactgg ctgccaccgt ttgccgcgca ggtattaaat aactcctggc aggtcgcagt
     9001 gtttggcagg ttcagagcca gtgccttcgc actttgcagt gccaggttga gatctttctg
     9061 gtgcagcgca attttgaaac ctggattaaa ggtgcgttta atcatgcgct caccgtgaac
     9121 ttccagaata cgtgaggaag caaagccgcc catcagcgcc tggcgcacac gtaccgggtc
     9181 tgcaccggct tttgaagcaa acagcagagc ttcagagacc gcttcaatat tgagcgctac
     9241 gataatctga tttgccactt tgcaggtttg accgtcgccg ttaccgccca caagggtgat
     9301 atttttaccg agcagttcaa acagcggttt aacacgttca aataccgctt cgtcaccgcc
     9361 aaccataatt gacaacgtcc cttcacgcgc gccgatttcg ccgccggaga ctggcgcatc
     9421 gagataatca ccacccagtt cattcacctg acgagcgaaa cgtttagttt caatcggtga
     9481 aatggagctc atatcaacaa tggttttgcc cttcagcgag gctttggtac aaccattttc
     9541 accaaacaga acttcttcaa cctgaggtgt gtccggcacc ataataaaaa tgatgtccgc
     9601 tgcttccgtt acctggcgcg cggtgtcgac attaacagca ccgagtgaca gtaattcatc
     9661 agcaaccggt ccaatggtcg tgacatgtaa ttgatgaccg gcacgcgcca gattaatggc
     9721 catcggagta cccataatgc ctaagccaat aaatcccagt ttcataatta acctcttttt
     9781 aaatgtcgct attcctgaat tcagacaaca cgatcccctg ccaacttagc agcctaaaaa
     9841 agcattgtct gaatagcgtt acgttttaac ggtacggatc catccagcgt aaaccggctt
     9901 ccgtggtggt ttggggttta tattcacacc caacccaacc gttgtagtca gaattttcga
     9961 ttaccttaaa gagataatca taattaattt ctccggtccc cggttcgccg cgatgcggat
    10021 tatcggcaat ttgcaggtga ccaattttat cagcccattg cgtcatagtg ttggttaatt
    10081 caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg cagcaaccaa
    10141 catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca ggaatatcaa
    10201 aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata ttcgcggcat
    10261 aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca gccggcgttt
    10321 taccgaccag acagttaata tttttattac ccagcgcacg ggcataacga atcgctgctg
    10381 ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg cgctcccccg
    10441 ccgcccagtc acccgccggt aaattgtgca gcgtatgttc gagtttatta ctcgccagca
    10501 cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg ccgcgaaaac
    10561 cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat aacatcgata
    10621 aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg aagcaggttt
    10681 cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa ttccatgacg ttatccagtt
    10741 cgctgcccat cgaaatattt gtcacacgct cgagaataac ttccacgact accggtaccc
    10801 gatattgcgc cattaaggct ttcgcctgtt caaaggccgg tgcaatatct tccggtttga
    10861 agacgcgaat agctttacaa cctaaacctt ccgctacttt tacgtggtcg acgccgtaac
    10921 cgttcacttc gctggagttg atattctcga aagcgagttg cacgcagtag tccatgtcaa
    10981 aggcgcgttg tgactgacga atcaggccga gataggcgtt attcaccagc acatggatgt
    11041 acggaatgtt gaactgcgcg ccaacggcta actcttcaat caggaactgg aagtcgaagt
    11101 caccggaaat cgccaccaca ttgcgtttcg gatcagcggc acaaaccccc agcgccgccg
    11161 gaatcgtcca gcctaacgga ccagcctgac cacagttgat ccagtggcgg tctttaaaga
    11221 catgcagcat ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca taacaaacat
    11281 cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc ggcacgttgt
    11341 cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcaatcggcg acccattctt
    11401 tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc agcagtgtca
    11461 gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcgg ccaatttgcg
    11521 tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct accgaaccgg
    11581 tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc agcaacgttg
    11641 cgttaccgta acgatgcgcg gtttgcagac cgaccatccc ggccatcagt tcatgatcgt
    11701 ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc agttcagcaa
    11761 actgttgtaa cagtgcagct gcgtcagcat tgattacccc gcccccggca acaatcaccg
    11821 gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg ctggcagcag
    11881 gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa
    11941 cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc aggtgaaatg
    12001 cctgttgcag cacgcgaggc accagcgccg cttcacgaac tgtaaccgcc attttgctga
    12061 ccggtttagc aattgcttca atatctacgg cctgaaaatc ttctttatgc agacgggcgc
    12121 gcggtgcctg gccggtaatg cacagaatag gaatggaatc agcagaagcg gaatagagcg
    12181 cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg atattccctg
    12241 ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga cgcgccagaa
    12301 tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt gcagctcccg
    12361 gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg
    12421 ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc caactttcaa
    12481 attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt
    12541 cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac
    12601 aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaacgct
    12661 acctacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat gctcttcttt
    12721 atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaggg tgggcatatc
    12781 cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc
    12841 ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca gtggcagacg
    12901 actgcccagt ggcgcgcaca tcctgaccat cgatttacac tctaactgac caattaatac
    12961 cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg
    13021 gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg ccgcaccgac
    13081 gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat agacaaaatc
    13141 cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag
    13201 agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat
    13261 tccccgctct aatgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc
    13321 gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat
    13381 gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt
    13441 tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg
    13501 gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta attcactccc
    13561 tgttcgccgt tggtaataaa cgcccgcagg gttgacaggt ctggtttgtc gtcacctaac
    13621 gctacgacca ccacaaacac ctcacctttc atcgggataa aggcctgagt acccagcgga
    13681 tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgctcg gttaatgctg
    13741 ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctccacc
    13801 aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc gccataagca
    13861 ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttgtaacat
    13921 aattccgttc aaccagcgcc gaccggtcag cttggtttca ataacaggca atctaaaacc
    13981 ttaagagaac aacatcaaca caaaacttgg aatatttttc caaaactaga tttagtccac
    14041 aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca
    14101 acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc
    14161 attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg taaaaccacg
    14221 gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtggc gctgtttttt
    14281 cgcacgactc gtagcgtaac gttgacagcg gctggcgagc atctactttc ccaggccaga
    14341 gactggctga gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg
    14401 gaacgccagg tgaatattgt catcaacaac ctgctctaca acccccaggc cgtcgcccag
    14461 ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc ccgacaaatc
    14521 tatatgggcg tctgggactc gctattgtac gaaggttttt cactggctat cggcgtcacg
    14581 ggaaccgaag cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcga
    14641 tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcgcag
    14701 ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac caaacgcgtc
    14761 gcctggcgat tgccagggca aaaagagatt attgttcctg atatggaaac gaaaatcgcc
    14821 gcccatctgg cgggcgttgg cattggtttt ttgccaaaat cgctttgcca gtcaatgatc
    14881 gataatcaac aactggtcag ccgggtaatc ccaacgatgc gccctccttc gccattgagt
    14941 ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac cttgtttacc
    15001 cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg cagttaaaaa
    15061 tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt
    15121 taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc
    15181 ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc gccgctatca
    15241 atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat aaacagcaag
    15301 gctcagacgc agcgctggcg ctgggacata aactggttgc gggtgaaatt cgtcagcagc
    15361 gcatggacgc ctggcgggca gcctgcctgc aaaatccgca aggtattctc tgctgcgccc
    15421 gtggcggtca gcgctcacat attgtgcaaa gctggttgca tgcagcgggg attgattatc
    15481 cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg actattgaac
    15541 tggcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa acgctgttag
    15601 tgcagcaaca gccgaacggt gttgatctgg aagggttggc gcgtcatcgc ggttcggcgt
    15661 ttggtcgcac gttacaacca caacttagcc aggcgagttt tgaaaacctg ctggctgccg
    15721 aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa gacgaaagcc
    15781 ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact caggcggcga
    15841 ttgcggtggt agaagatccg tttgagatcc gtcttgagcg cctgaacgaa gagtatttct
    15901 tgcgtatgca tcatgatttt acccacgcgt acggcgacga acagggctgg caggaatatt
    15961 gcgaatacct gcatcacgga ctttcggcta ttaagcgtcg gctggggcta cagcgatata
    16021 acgaactggc tgcaaggctg gatgcagcac tgacaacgca actcaccacc ggcagcaccg
    16081 acggtcatct ggcctggctg gtgccgttac ttgaagaata ttacgacccg atgtatcgct
    16141 atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg
    16201 aatgggttaa ggcgcagtaa tgagggacgt atttgtatta gtcgcgactt gatctgacac
    16261 atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc ttatccaaaa
    16321 caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa agccggtttg
    16381 ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat gggcgtttcg
    16441 cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga tgcgcagatt
    16501 aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac tgaacaggct
    16561 gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag caacgaactg
    16621 cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt actccataac
    16681 cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc cgtaaaaagt
    16741 tgtatggagt tctcgatgca gtcggcgcga ctataagtac ccccgctgaa ccctggacta
    16801 tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg ctaatggttt
    16861 atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat ttattttcag
    16921 atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat atgtattaca
    16981 caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga ctgttcattt
    17041 tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg agtagatatt
    17101 catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca gtcaggaatt
    17161 ttacttttcc tcactacgaa gttgacgact tcgatatggg atagactctt aattcaagca
    17221 attactctgc acaagcatca ggcaatccag ctccaatatt tgttataaaa taatgcgtaa
    17281 cattaatttc accaacaaaa aaaggtttac accatcccct tgccctggta tcttaccata
    17341 tttacaccac actttttttg tatctggatc attctttgat acgattttcg ccacttgttc
    17401 cctggccctc actgtcaatg ttttcttaaa cctcccccca tcatcatttg ttagggaaaa
    17461 ataagaaaaa aataatcatc ttctacattt gtgtatattg atatatccac cgctacgtgc
    17521 ggaaaaaaat ctgggagcca tgtaaaaact ccatca
//
LOCUS       DAZGXS010000098        17050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGXS010000098 DAZGXS010000000
VERSION     DAZGXS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..802
                     /gene="espX2"
                     /locus_tag="ACHKG5_003375"
     CDS             <1..802
                     /gene="espX2"
                     /locus_tag="ACHKG5_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP0869411.1"
                     /translation="DNKCYILLSSDNSGRVMRLSHQALISMLEPEVKKKTIWNNYSIY
                     PSLQDTHEVVRDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSA
                     IMRKTDFVLECNNQPTELNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESD
                     KSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTY
                     KTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1086..3236
                     /gene="dinG"
                     /locus_tag="ACHKG5_003376"
     CDS             1086..3236
                     /gene="dinG"
                     /locus_tag="ACHKG5_003376"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0869412.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3264..4226
                     /gene="ybiB"
                     /locus_tag="ACHKG5_003377"
     CDS             3264..4226
                     /gene="ybiB"
                     /locus_tag="ACHKG5_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0869413.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4367..5452
                     /gene="hcxB"
                     /locus_tag="ACHKG5_003378"
     CDS             4367..5452
                     /gene="hcxB"
                     /locus_tag="ACHKG5_003378"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0869414.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5519..5578
                     /gene="ybiE"
                     /locus_tag="ACHKG5_003379"
     CDS             5519..5578
                     /gene="ybiE"
                     /locus_tag="ACHKG5_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP0869415.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5681..5941)
                     /gene="ybiJ"
                     /locus_tag="ACHKG5_003380"
     CDS             complement(5681..5941)
                     /gene="ybiJ"
                     /locus_tag="ACHKG5_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0869416.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6206..6472)
                     /gene="ybiI"
                     /locus_tag="ACHKG5_003381"
     CDS             complement(6206..6472)
                     /gene="ybiI"
                     /locus_tag="ACHKG5_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0869417.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6546..7232)
                     /gene="ybiX"
                     /locus_tag="ACHKG5_003382"
     CDS             complement(6546..7232)
                     /gene="ybiX"
                     /locus_tag="ACHKG5_003382"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0869418.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7274..9556)
                     /gene="fiu"
                     /locus_tag="ACHKG5_003383"
     CDS             complement(7274..9556)
                     /gene="fiu"
                     /locus_tag="ACHKG5_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0869419.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9821..10081)
                     /gene="mcbA"
                     /locus_tag="ACHKG5_003384"
     CDS             complement(9821..10081)
                     /gene="mcbA"
                     /locus_tag="ACHKG5_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0869420.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10357..11283
                     /gene="rlmF"
                     /locus_tag="ACHKG5_003385"
     CDS             10357..11283
                     /gene="rlmF"
                     /locus_tag="ACHKG5_003385"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0869421.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11280..13505)
                     /gene="ybiO"
                     /locus_tag="ACHKG5_003386"
     CDS             complement(11280..13505)
                     /gene="ybiO"
                     /locus_tag="ACHKG5_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0869422.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVAHYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13622..14344)
                     /gene="glnQ"
                     /locus_tag="ACHKG5_003387"
     CDS             complement(13622..14344)
                     /gene="glnQ"
                     /locus_tag="ACHKG5_003387"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0869423.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14341..15000)
                     /gene="glnP"
                     /locus_tag="ACHKG5_003388"
     CDS             complement(14341..15000)
                     /gene="glnP"
                     /locus_tag="ACHKG5_003388"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0869424.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15138..15884)
                     /gene="glnH"
                     /locus_tag="ACHKG5_003389"
     CDS             complement(15138..15884)
                     /gene="glnH"
                     /locus_tag="ACHKG5_003389"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0869425.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16288..16791)
                     /gene="dps"
                     /locus_tag="ACHKG5_003390"
                     /gene_synonym="pexB"
     CDS             complement(16288..16791)
                     /gene="dps"
                     /locus_tag="ACHKG5_003390"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0869426.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         4186 a         4213 c         4490 g         4161 t
ORIGIN      
        1 agataataaa tgttatatcc tgttatcatc cgataactct gggcgagtaa tgcgcttatc
       61 gcaccaggcg ttaatctcaa tgcttgagcc agaggttaag aaaaagacta tctggaataa
      121 ttactctatt tatccatctt tgcaggatac ccatgaagtt gtcagagatg atccagaaac
      181 gatttgtatg cgtgcatttc ctttatttgc aaaaggatgg gaatatgcgc agaaaaataa
      241 gaaacatcaa ttgatcctca atgctcttgg attcaaaggt tatattcgtg atatatttat
      301 gtcagcaata atgcgaaaaa cagattttgt gctcgaatgc aataatcaac cgacagaact
      361 taattcatca ttttcctctt tgatgaacga ttcagatcaa tggcaacagc atactctgaa
      421 agataaacat tacgctaatc tattaacgat gctggatctc aatgatgcca gtgaaagtga
      481 taagtctaaa atatttttct gcctgtctgc cgtatttgca aatatttctc acagtaatgt
      541 ctttaacggt attcctgatg cgtcaaaaac tttaaaacgt tatgcatttg ctttattggc
      601 gaaagctcat tctttagatg aaagtatgat aagcaatcaa acttttaata catacaaaac
      661 ggtattgctt gatttcaata atctgtctaa tgaagaggcc aaccagttgc gcataagttc
      721 gttatatcgt gatatggtcc gctatgcaca atatcgtttt tctaaagttt tatccgagtg
      781 gacgccagac gcatggctgt aaagatcgca gcagaataat ggcattgatg gccctggctc
      841 aggccagggt cggcaatacc ggttcctgcg aagcgatgac ggcggcaatt gtaaccacga
      901 aatgcggtat cacttgctgt tttctgccct gaagactggc gctcactgtg ccgtcgaatt
      961 ttcaatcaaa tatattgcga gagggcgtgt ttgcgagccg ctttccagaa acagaaaaac
     1021 cattacccct gaaaaccgaa aaatgccaca atattggctg tttatacagt atttcaggtt
     1081 ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc
     1141 aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt gcggacgtcg
     1201 ccaaaacgct ggccggagaa gaagggcggc atctggcgat tgaagccccc actggcgttg
     1261 ggaaaacgct ctcctatttg attcccggca tcgccattgc ccgcgaagag caaaaaacgc
     1321 tggtggtgag taccgccaac gtggcattgc aggatcagat ctacagcaaa gatttgccac
     1381 tgctgaaaaa gatcattccc gatcttaaat tcactgccgc ttttgggcgt gggcgctacg
     1441 tttgcccgcg taatctgacg gcgcttgcca gcactgagcc cacgcaacag gatctgctgg
     1501 cgtttcttga cgacgaactg acgccaaata atcaggaaga gcaaaaacgt tgtgcgaagc
     1561 tgaagggcga tctcgacact tataaatggg atggtctgcg cgatcatacg gatatcgcta
     1621 tagatgacga tctctggcgt cgtttaagta ccgacaaagc cagctgcctc aaccgcaact
     1681 gttactacta tcgcgaatgc ccgttttttg tcactcgtcg ggaaattcag gaagcggaag
     1741 tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc gtattgcctg
     1801 acccgaaaaa tttactgctg gtgctggacg aaggccatca cctgccagat gtggcgcggg
     1861 atgcgctgga gatgagtgcc gaaatcaccg cgccatggta tcggctacag ctggacttgt
     1921 tcacgaaact ggtcgctacc tgcatggagc agtttcgccc gaagaccatc ccgccgttgg
     1981 cgatccctga acgtttgaat gcgcattgtg aagagctgta tgagcttatc gcctcattaa
     2041 acaacattct caatctctac atgcctgccg ggcaggaagc agaacaccgt tttgcgatgg
     2101 gcgaactgcc tgatgaagtg ctggagatct gccagcggct ggcaaaactc accgagatgc
     2161 tgcgtggtct ggcggagtta tttcttaacg atttaagtga gaaaaccggc agccatgaca
     2221 ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc gttggggatg ttcgaggcgc
     2281 aaagcaaact ctggcggctg gcttcgctgg cgcaatcttc cggtgcaccg gtgaccaaat
     2341 gggcgacgcg ggaagagcgc gaagggcagc tacacctctg gtttcactgc gtgggaatac
     2401 gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt attgtcacct
     2461 ccgcaacctt gcgttcgctg aacagttttt cgcgtttgca ggagatgagc gggctgaaag
     2521 agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc gaacagggca
     2581 aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagaa cagcatattg
     2641 ctgaaatggc ggcctttttc cgtgagcagg tggagagcaa aaaatatctc ggtatgttgg
     2701 tgctgtttgc cagcgggcgt gcgatgcagc gctttctcga ctatgtgacg gatttacgtc
     2761 tgatgttgct ggtgcagggc gatcagccgc gttaccgttt agttgaactg caccgcaaac
     2821 gcgtcgccaa cggtgagcgt agtgtgctgg tgggcttaca gtcatttgcc gaagggcttg
     2881 atttgaaagg tgatatgctc agccaggtgc atatccacaa aatcgctttt ccgcctatcg
     2941 acagcccggt ggtgatcact gaaggggaat ggctgaaaag cctcaaccgc tatccatttg
     3001 aggtgcaaag cctgccgagc gcctcgttta acctgattca gcaggttggg cgactgattc
     3061 gaagtcacgg ttgctggggc gaagtggtga tttacgataa acgcttgctg accaaaaatt
     3121 atggcaagcg actactggat gcattaccgg tatttccgat agagcaaccg gaagtccctg
     3181 aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat
     3241 gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag atcgggcgcg
     3301 ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat gcccatatgc
     3361 tcaatggtga agtccctgac cttgagttgg gcggtgtatt gattgcgctg cgtatcaaag
     3421 gggaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac accatcaagc
     3481 tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta
     3541 aacaggccaa cctgacgccg ttgttggcga ttctcctgca caaactcggt tttcctgtgg
     3601 tggttcacgg ggttagcgaa gatccaactc gcgtgctgac cgaaaccatt ttcgaactga
     3661 tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac gaacatcaac
     3721 cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct
     3781 ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg ccatttgccg
     3841 aaggtgaggc actgcgtctt tccagcgttt cgcatccgga atacatcggg cgagtcgcga
     3901 agttctttag cgatatcggc gggcgggcgt tactaatgca tggtactgaa ggcgaagtgt
     3961 atgctaatcc gcagcgctgc ccacagatca atctcattga tcgtgaagag atgcgggtgc
     4021 tgtatgaaaa acaggaccct gctggaagcg agttactgcc acaagcaaaa gatccggaaa
     4081 ccacggcgca gtggattcag cgttgccttg ctggccgcga accgattccc gaatcgctga
     4141 aaatccagat ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc agcgatggcc
     4201 tggcgcgcgt taatcaggca ttttaattct tttctcagcc ggatgagcca tgtcatccgg
     4261 cttttacccc accgcaatat gaaattcctg catctttatt gaccttcccg cgcccggcgt
     4321 gcagcataaa aaaacaacaa acacataaca taaacaggag ttaaccatgg aaagtggtca
     4381 tcgctttgat gctcagacgc tgcacagctt tattcaggct gtatttcgtc agatgggtag
     4441 cgaggaacaa gaagcgaaat tagttgccga tcatttaatc gcggcaaacc tggcagggca
     4501 tgattcacat ggtattggca tgatcccaag ctatgtacgc tcctggagtc aggggcacct
     4561 gcaaattaac catcatgcca aaatcgttaa agaggcgggg gcggcggtca cgctcgatgg
     4621 cgatcgcgca tttggtcagg tcgcggcaca tgaagcgatg gcgctgggga ttgagaaagc
     4681 gcatcagcac ggtattgctg ccgtggcgct acataactcg catcatatcg gccgtatcgg
     4741 ttactgggcg gagcagtgtg cagcggcggg gtttgtctct atccactttg ttagcgtggt
     4801 cggtattcca atggtcgcac cgttccacgg tcgcgacagc cgctttggca cgaatccgtt
     4861 ctgtgtggtt ttcccacgta aagatgattt tccgctgttg cttgattacg ccaccagcgc
     4921 cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg
     4981 cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg agtcgccgtt
     5041 gggttcgctg ttgacctttg ccgaacataa aggctacgcc ctggcggcaa tgtgtgaaat
     5101 tcttggcggg gcgctttctg gcggtaaaac gacgcatcag gaaacgttac aaaccagtcc
     5161 cgatgccatt cttaactgca tgaccactat catcatcaac ccggaactgt tcggcgcgcc
     5221 ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga
     5281 cgataagccg attttgctcc cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagga
     5341 gcaggggatt ccattggatg cgggaagctg gcaggccatt tgtgatgcgg cgcggcagat
     5401 tggtatgccg gaagagacgt tgcaggcttt ctgtcagcag ttagccagct aaaaaaaagc
     5461 ccgtccagtg gcggacgggc aaacaagggt aacataggat caatgagggt tagagcatat
     5521 gcgtctgtcg gcaaacagac agggaaatac ttgtgcagga cgtagcgtaa acgcctgatc
     5581 cgtccaacgg ttcggatttt gtaggcctga taagacgcgg tagcgtcgca tcaggcatct
     5641 gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa attaccgcag
     5701 taccgcttaa tttattgttg ttggtggcgg aagtgatgct atatccactg gcaccggctg
     5761 ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg ccatcggcag
     5821 aaaccacgcc gattttattc atgttctgtg cctgggatgc cgttaccggt tccgcggcaa
     5881 acacgccaaa tgacagagct gaaagagcca tagcagcaac aacagtattg atagttttca
     5941 taattaatct ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga tgtgatgagt
     6001 atcacgtttt tttacgagag ataaaatcga agagaattga ccgccttgtt caaataaatt
     6061 gattgatatc taacatactg ataaaccatt tatttgttgt acttatggac ttgttttatg
     6121 cagcgtggca gtcacgacag cgttaaaggc acggaatgcc acgcaaaata caaattatac
     6181 tcgctataca tctcgccagt tgtagttaac gtaactggct ttctttcgaa cctctgcgat
     6241 tatatcctgt ataagctggt ttttgtaaat ctttctcctg ctgacaatga atacataagc
     6301 gcacgccagg aatggcttcc cgacgggctt gcgggatggg ggcaccgcac tcttcacatt
     6361 catacaggct ttcgccacgc ggaatttcac cccgagcgcg ggcaatcgca tcttcaattg
     6421 tactgttgat ctgttcgttg acggcgtcat cgttagccca accggatgcc atatcgtcct
     6481 ccccatatca atgcttgtat agttaagtgt agctaatata gggacgaacc tgggcagttc
     6541 aagcgtcaga tctccgacca ttcccgcagc agattatgat aaagattgag cagcgacagg
     6601 atctcttcat tttcaccata gcggcttttc agcgactgaa tattctgaat atttttgtcc
     6661 agttcaaaca gcatggcgcg ctttttatca tcgcggatca tcgactggat ccatataaat
     6721 gatgccactc gtacgccgcg ggttacgggt gtcacgcaat gcaggctgct ggagggatac
     6781 aacaccagat cacctgccgg gagttttacc cgatgttgtc cgaaggtgtc attaacgacc
     6841 agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagtg
     6901 cgcatccagc cgttttgtgg atggctgcgt actgcgccat ccacatgaaa accataggtt
     6961 tcattgttct gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggccgcggca
     7021 aagaacaaag catgttggtt aaccgcgttc agcacctcat tttgcagggc ggcgtataac
     7081 gtgctgcggg tgtctacctg ttgattgttc ttaacttgcg caccttgtgc gccggtggtg
     7141 acgcgtccat ccacccattc ggcttgttcc agttgttcgc gaaaacgggc gacgtcctgt
     7201 ggcgataaca cgccgggaat gtggtacatc atacaagttc tccaaaaagt ggggcctgcg
     7261 ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg
     7321 ggtgataacg gtagccgctc ttattgattg aggcgacgta atcggtatca aacaggttgt
     7381 agacgtttag ctggaagtcg agattgcgat taaccctata ccccagtttg gcatcggcga
     7441 cccagtaacc ttcggtaaac gctggcgttc ccaccgcgcc gtctgaacct ttatgcatac
     7501 tgccgatata gcgtgcgccc gcgccaacag agatatcatc ggttgcctga tattggctcc
     7561 ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc tgggcaacat
     7621 ctttgccgtt tttgatggtt gctttttgct gggtatagcc gccaatcacc tgccacgcgg
     7681 gagtgatatt ccctgccacg gatatctcat agccttcgac gcgtttctta ccgtattgcg
     7741 agtaagtccc gtcatcattt tgctcaactt cattttcgat atcagtacgg aacagcgcgg
     7801 cggtgagcaa caggcgttta tccagaacct gccatttggt gccaatctcg ctggtgttgg
     7861 ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc gctgccagac tgcgcaaggg
     7921 cgaagttgtt gccgcccgga ggctgctggg aaacggcata gttaatatag acattgccgt
     7981 tttccgtcag gtgatacagc gccccggctt tccagttcac cagattgccc gacttggcgg
     8041 tgtcgacggt ggtgaccgga gaaccttttg ccacaccagc tgggcaggtg atggcaccgc
     8101 gtccgctgcc gccgcaggcg gtggcactgt catattcagt atgataatta tccagacgga
     8161 tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt atcgaatgcg taaattgcga
     8221 aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg ggatgaatgc
     8281 tgctgtcagg atgataaata tttaccgcag gtaacgtcac cggattaacg ccgtagttag
     8341 tctgcgtttc acgggtaaat tccacgccgg tactgacatc atgaccgata gaagcggtat
     8401 agaacgtcga ggtcaggttg gtctggttgg tgagaatttt attactcaca tctttggtat
     8461 tcgccgtgcg tgaccacgtc cagctattca catcgctggt gggctgggta atattcgacg
     8521 ccccacccat aatcgccgtc atcaggtaat cctgcttcac gcgcgaccaa cgggtagtgt
     8581 tgcgaatggt ggtgttatcg ttgatatcgt gctcaaaacg catggtggcg gtgtcggtgg
     8641 tcgaatcgtc gtaatcggaa tccgtgccgt aaaagttatg cgtatcaact tttccggaat
     8701 gattcagggt cgccgttcct gcagatgggg cagaatagcc cggcaaaccg atggttggaa
     8761 tgccgccgtc tggcgtgttg tgctgggtga catgcagata attaagatac aaacgattcg
     8821 ctgtaccaag gccaaaagcg atagaagggg cgacgccgta acgctcattt ttgactttgt
     8881 cgcgtccggc atcgtgcgtt ttttcgccca tcacattcag gcgcaccgca gtggtatcac
     8941 caatgacctg attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca atactggcgg
     9001 aggcgtcaat gccggaatca ttgcgcggct gcttgctgat catattgatc gagcccgtcg
     9061 gcgcgctgcg cccgtagtcg gtgccggaag gccctttaat cacttcgacc tgctcagtat
     9121 tgaaggtgtc gcgcgagacg ctgccgatat cgcgaatgcc atcaatataa atactgttag
     9181 aggtatcagc accgcgcata taaatggcgt cgccagtggt ggagttaccg ttctcacccg
     9241 caaaaaacgc acccacgccg gggacgtttt tgagcgcatc ggtaaggttg gttgcgccct
     9301 gatctttaat cacttgttca gaaatcaccg tcatcgtgcg ggtagtatcc gctaccggac
     9361 gcgagaattt cggatcggca gattgttgtg gcgcataaag cgaaggcgtt gatgcttcga
     9421 caaccagcgt gtcatccgcg ttagtttgcc cttcggcggc gagtgcctga gccacaggcg
     9481 tgatgccaat acaaagaccg gcaaagaacg tgagcgaatg aaattgtctg gcagggaaat
     9541 tgcgattgtt ttccattttg caggtgactt tttcttatat gttgaaattt tcactgccac
     9601 gagaaagcct ctctgcggat gcgatgccac tgttaatctc gtgatagtgt tcgtgcaagt
     9661 gataataatt ttgataagca ttatcacttg gcgcgatttt ctatcaccca ttgcgcgaaa
     9721 aagaaataca tttatttaca ttgcgttcat atttgatgtt ttttgattac aaaacaggcg
     9781 aaaactgcat ccagaggcga atgcagttac gttggtgggg ttatttgtag atggttgcag
     9841 taccgaacat ctggtcattt ccgcctgcgg aattaatgac ataacctttc gcgccttgct
     9901 cgcgcgcttt ttctgccagt ttatcctcca gatcgcttag gtttgatgcg ccagttgccg
     9961 aaacggtacc ggcgggccgt agctgaccgc tgtccaggtt actcatcggt tgcgcggcat
    10021 aagccgtgag agagattccg ctcaggacgg tggcaatcag tagtgtgagg cactttttca
    10081 taatgacatc ctcataaata caacgggtag tgcctgataa gtgtaggtcg cctgcgcgat
    10141 gcaaatgacg caaaaaattg acagagatgt gcttattttt tgaacgatat atttttacaa
    10201 aataagacaa atcaatgaca tgcataatgc tgatttttat gatgaattgc cataaaccat
    10261 agccatgtta aggttttggg gaatgataaa cgcagccaga tatttaccct gtcgcgttat
    10321 gcgggtatct tacgccgctg cttaaaggag aatgctatgt ccgcccagaa accggggttg
    10381 catccgcgca accgtcatca cagccgctac gatctcgcca cgctttgtca ggtcaatcct
    10441 gaactcaagc aattcctcac gcttacaccc gccggggagc aaagcgtaga ctttgccaat
    10501 ccgctggcgg tgaaggcgct caataaggcg ttgctggccc atttttacgc cgtagcgaac
    10561 tgggatatcc cggacggttt tctctgcccg ccagtaccgg gccgggcgga ttatattcat
    10621 caccttgccg atttactggc tgaagcaagc ggaacaattc cagctaatgc cagcattctt
    10681 gatatcggcg ttggtgcgaa ctgtatttat ccgctgattg gcgtacatga atatggctgg
    10741 cgttttaccg gtagtgaaac cagcagccag gcgttaagca gcgcgcaggc gatcatcagt
    10801 gctaatccgg ggcttaatcg cgccattcgt ctgcgtcggc aaaaagagag tggggcgatt
    10861 tttaacggca tcatccataa aaacgagcaa tacgacgcga ccttgtgtaa cccgccattc
    10921 cacgattccg ccgctgcggc gcgggcaggt agtgagcgta aacgccgtaa cctggggctg
    10981 aacaaagacg atgcactgaa ctttggcggc cagcaacagg agttgtggtg tgaaggcggt
    11041 gaggtcacct ttatcaaaaa gatgattgaa gagagcaaag gcttcgcgaa gcaggtgatg
    11101 tggtttactt cactggtatc tcgtggtgaa aacttaccgc cgttgtatcg tgccctgacg
    11161 gacgtgggcg cggtgaaggt ggttaaaaaa gagatggccc aggggcaaaa gcagagtcgc
    11221 tttattgcct ggacctttat gaacgacgag cagcgccgcc gttttgtcaa tcgccagcgt
    11281 taaagcgttg gctcccccgg cggtaacggt tcagccgggg tcgcgcccgg agcaggcagc
    11341 acctgataag tctgcaccgg cgcgcgaacg cccgccaggt cgaaatgttt tttcacctgg
    11401 ctgtcgaggg caaagcgtac cgtccactgt ttgagtggca gcgtggtgaa cgaaacacgc
    11461 agtgtaaacg cggtattgct taagccaaca atcccggcaa agttcggttc accaataatc
    11521 agcccacgaa tttcttcgtt ttccattaat tccgctaccg catctttcag tgcctgattg
    11581 gctttatcag catcttcatg gcgatcaaca tcgtagtgtg ccactaccga accaatgccg
    11641 cggacaaagt tggcaaaggt ggtaatcgaa gaccacggaa tgatgtggta cgccccggta
    11701 tcctggcgta cgcccacgga gcgaatcgac atccgttcca cagtgccggt caacggccca
    11761 atagtcacca aatctccagt gttcatgccg ttttcaaact gaataaatac cccggtgata
    11821 atatctttca ccagcgtttg cgaaccaaac gagatagcca gacctaatgc cccggcacct
    11881 gccagcaatg gcgcgatatt gacgccgatt tccgacaaca caatcatgat ggtgatggta
    11941 ctgataatca ccgccagcgc gttacgaaac agcgtaagca gggtacgcgt gcgggcgctg
    12001 ggtagcgggc ggccatgaat atccgaagcc agccggtttt cgatcaaact ggcgagcacc
    12061 gtccagccaa ccgccgagaa gaaaagaatg agtgcgatac ggatcaggat atctacggtt
    12121 ttctgccccg cgccgttttg cagccagttc cagaaatcga acaatcccca tgcgctcaac
    12181 agcagcatta ccgccacgca gacggtcaga atacgcgccg ttttcagcgc cgccgaaagc
    12241 cagccattca gccgtttttg caactccgga tagttacgct gagtatgtgg cgagagagtg
    12301 atggtttttg ccagccagcg ggaaaacata ccggaaacaa atgctgcgat accaataatc
    12361 gccaggctgc gcactgttgc acccatcata aatttcaggc tgttgcccgg atcgaacaac
    12421 gaaaagaaaa acagcacgat aaaataggcg cttgccagcc agtgccacac cagcgcaaag
    12481 gcgcggataa acaggctgaa aaaggccagc gaatgctccg caaagttgag caaatgctgg
    12541 gtaatctctt ttttattacg aaagatcagg tacaacgccc agacggtcat gcacagcata
    12601 atgatgacgt tcgccagcgc acctatctgt acattcacct gattagagat aatcggcacg
    12661 gccacaatca ggccataacc tatcaaactg cctaaccagc tcaggcgacg actccagtaa
    12721 cgggcgctct cgtcctgaat cgtgaacggg cgcagttccg ccacgtttgg gcaaaaaatc
    12781 aggcgtagta cggctttgaa aaattcaatc agggcaaagg cgttgagaaa caaactttgt
    12841 tggaaagcga tggtgcgact gcctgcattc aggttgtcgc ttaatatctg gccgacaaat
    12901 aatgtcagtg ccagtaacag caggtcgata ataaacgccc cgataatcat cgccggaagc
    12961 tgcagccagt tactgcgctc acgatttttt tgccgcgccc actgacccat tttgcgatac
    13021 agcggcagtg cgcacaggcg aatcagccag taaaaaccaa acactaaaac cgctaacatt
    13081 gaaaaatggg tcagcgcatt gctgaaggtt tgtggattaa acggcttatg cggggagccg
    13141 gtgatattgc gataaagctg cccgaagcgg gcggaaaggg cttcaccata atggcggctg
    13201 aattcggtga ccttttgcag cacggtttgc tcttcaacca gcgtcggcgg cacgatcttc
    13261 gggaccggtt cagcaggtgg cgtcgcggcg acggtgcgca actggtcgat caactcttta
    13321 cgcgaggtgt cattatccag cacatccgcc agtgcgccat aagccgcttt tttttgttcg
    13381 atatccggtt ccggggccgg ttcagtcgtt gagtccgttg tcgttgtggt tgtaacgccg
    13441 ggtatggata ccgcgtgggc aggtgcgccc agcaggcaga agaggatgaa caggatccac
    13501 cgcatgactc ctccagtgag aaaacagagc aaaaagataa gtatagatgc tgggggaggg
    13561 gtgattttaa atgagaggaa tctggtgtac ctccctttcg ggtgaaaggg aggcagagaa
    13621 gttaagagac gtgctgtaaa aattcctgca agcgctggct cggcggattt ttaatcaaca
    13681 cttgtggatc gccatcttcc gcaatccggc ctttgtcgat aaagatcagg cgcgaagcta
    13741 ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct tcttcagcca
    13801 gatcttgcat aaccttcagc acttcatggc gcagttccgg gtcaagagcg gaagtcggtt
    13861 catcaaacag catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc acacgctgct
    13921 gttgaccacc agaaagttcg gaagggtagt gatgtgcacg ttctgccaga ccgactttcg
    13981 ccagcagctc acgtgccagt ttttccgcct cttctttgtt cgcgccacgc acgcgtagcg
    14041 ggccaaacat gacgttttcc agcgccgtca gatgcgggaa gaggtaaaac tgctggaaca
    14101 ccatacctgc ttcctggcga atcaggcgct cgtcaacttt cggatcgtta accttcaggc
    14161 catcgacaat cagatcgccg gaggtgattt cttccagttt gttgatgcag cgcagcaggg
    14221 tcgatttacc ggaaccggac ggcccgataa tcaccacgac ttcgccctgg gcaatgttca
    14281 aatcgatatt gtgcagcacc tgggttgggc caaagtgctt ggagacgttt ttaaattcaa
    14341 tcacaggatt ttcatccttc tttccagacg acgcagaata aagctcagca ccagggtaat
    14401 aatcagatag aacaccgcca cggcgctcca gatctcaagg gcgcggaagt taccggcaat
    14461 aatttcttgc ccctgacggg tcagttccgc cacgccgatc acaataaaca gcgaggtgtc
    14521 tttaatgctg atgatccact ggttacccag cggcggcagc atacgacgca gtgccagcgg
    14581 taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc ctgcttcacg
    14641 aaaacctttg tggatagaca gcaccgcacc acgcgtgatt tccgcaatgt acgcgccgga
    14701 gttgatcatg atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt cattaaacgc
    14761 catcggcagg gcaaaataga taaacatcac ctggacgacg ataggtgtgc cgcggatcac
    14821 ttcaataaaa accagcgcga cgtggttggc tatccaacca ccgaaggtgc gtgcaaaacc
    14881 tgccagcaat ccgatcacca ggccgccagc cagaccgagg accgatatcc acagggtcat
    14941 tttggcacct tcaatcagaa gcggaatggc aggccagata gcactccagt caaactgcat
    15001 atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat ggccccggag
    15061 cgggtttgcg ccctcaccct aatcctctcc ccagagggga gagggaactg ccagtgcgct
    15121 ttacaggtgt agcgttatta tttcggttca gtaccgaacc attttttgta gatttcgttg
    15181 taagttccgt tctcgcgcag ggttttcaac gcgccgttga ctttgtcacg cagctcgtcg
    15241 ctacctttcg ggaacgcaat accgtattgc tgcgcttcca gagagtcgcc taccgctttg
    15301 aactgaccgt taccggcggt tttgatgaag tacagaatgt ttggcgtatc gtgcagaacg
    15361 gcgtctgcgc ggttggtgcc cagttccata taggcgttat cgatgttcgg gaactgacgc
    15421 agatctttag ttttgatgtt tgctttcgcg taatcaacgg agccagtgcc gctcttcacc
    15481 gcaaccactt tcccgtcgag atctttcacg cttttcacat cgttattgtt agctttcacc
    15541 atcactaaca ggccgctttt gtagtagccg tcagagaaat cgatcgcttt tttacgctcg
    15601 tcggtgatgg taatgcccgc cagcgccaga tcgacgtttt tggtttgcag tgccggaatg
    15661 atcccactga aatccatcgg cttcagttcg taatccagct tcagctcttt agcgatggca
    15721 gcccacagat caacgtcaaa gcccacatat ttatcgccct gtttaaattc aaacggaacg
    15781 aaggcggtat ccgtcgcgac aactaatttt ttatccgcgg catgagaaga aaccgcaaaa
    15841 gccagggtca gtgcagccag tgaaactttt aatacagact tcatagcatt tccttttttc
    15901 tatccacggg acgatcccct gcgtgacatt cacatatatg aaaaaatcgt gccagttttg
    15961 caactcattg atttttcgag atgcggcaaa aacgtgatgc acgatttata gggcaacacc
    16021 ctgaagatgc accattctgg ggcaccaaac tggtgcgcta aaattgtgca ctcaacacag
    16081 tgattattta gcgtaaaacc tgatgaaaaa acaatcttta tgtaacgatt gtgtgatgat
    16141 gtggatacaa aaaatttact taatcagctg gggatagcag atggatgcac taaataagtg
    16201 cgttgaggcg ggctgcaaaa caaaacggcc tcctgtcagg aagccgcttt tatcgggtac
    16261 taaaattctg caccatcagc gatggattta ttcgatgtta gactcgataa accacaggaa
    16321 tttatccagg tcgcgagacg cggcggtcag gatatctgcg gtgtcgtcat ctttcgcttc
    16381 gccaatcgct ttgcgtacgt cattagcgac gattgcgtaa cggtcagcca gttctttcag
    16441 gtgatcctga acgttgtgga tgtccagcgg gtaacttttc agcggggttt tgctgttgat
    16501 aacttgagtg gtccccagag ctacaccgcc cagctgcact gcacgttctg ccatggtatc
    16561 cagatgatcg atcagtgcag tgcggaagcc atccagcatt tcatgtacgg caatgaagtt
    16621 agcgccgcgc atgttccagt gcgcttgttt ggtaatcaaa gaaagatcaa taaactggat
    16681 aacctggcga ttcagcaact ctactgttgc ttttttctcg ctgtcagaga catcgttgcg
    16741 ggtataaagc agattggtcg cttttgattt aactaattta gcggtactca taatttcata
    16801 tcctcttgat gttatgtccc agtaattaac gagactaagt atagcaccgg ctatgtgttc
    16861 cgctattctg gctgttccta tcacactaat agtggtaaca agcgtgaaaa acaaaactaa
    16921 taaagattca atgagttaga tatattgata agaacaattc ttatttattt gctcgccagg
    16981 aattttcgga ataaatatgc ttttgcataa ttcgatatat aaagacggtg tagaggaaaa
    17041 gtagcgagaa
//
LOCUS       DAZGXS010000099        16912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGXS010000099 DAZGXS010000000
VERSION     DAZGXS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            105..2801
                     /gene="stcE"
                     /locus_tag="ACHKG5_003391"
     CDS             105..2801
                     /gene="stcE"
                     /locus_tag="ACHKG5_003391"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFP0869427.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2888..3763
                     /gene="gspC"
                     /locus_tag="ACHKG5_003392"
     CDS             2888..3763
                     /gene="gspC"
                     /locus_tag="ACHKG5_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP0869428.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3764..5731
                     /gene="etpD"
                     /locus_tag="ACHKG5_003393"
     CDS             3764..5731
                     /gene="etpD"
                     /locus_tag="ACHKG5_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFP0869429.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5731..7236
                     /gene="gspE"
                     /locus_tag="ACHKG5_003394"
     CDS             5731..7236
                     /gene="gspE"
                     /locus_tag="ACHKG5_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP0869430.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7238..8461
                     /gene="gspF"
                     /locus_tag="ACHKG5_003395"
     CDS             7238..8461
                     /gene="gspF"
                     /locus_tag="ACHKG5_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP0869431.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8492..8926
                     /gene="gspG"
                     /locus_tag="ACHKG5_003396"
     CDS             8492..8926
                     /gene="gspG"
                     /locus_tag="ACHKG5_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP0869432.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8923..9477
                     /gene="gspH"
                     /locus_tag="ACHKG5_003397"
     CDS             8923..9477
                     /gene="gspH"
                     /locus_tag="ACHKG5_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP0869433.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9474..9839
                     /gene="gspI"
                     /locus_tag="ACHKG5_003398"
     CDS             9474..9839
                     /gene="gspI"
                     /locus_tag="ACHKG5_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP0869434.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9836..10435
                     /gene="gspJ"
                     /locus_tag="ACHKG5_003399"
     CDS             9836..10435
                     /gene="gspJ"
                     /locus_tag="ACHKG5_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP0869435.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10432..11409
                     /gene="gspK"
                     /locus_tag="ACHKG5_003400"
     CDS             10432..11409
                     /gene="gspK"
                     /locus_tag="ACHKG5_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP0869436.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11343..12620
                     /gene="gspL"
                     /locus_tag="ACHKG5_003401"
     CDS             11343..12620
                     /gene="gspL"
                     /locus_tag="ACHKG5_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP0869437.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12607..13119
                     /gene="gspM"
                     /locus_tag="ACHKG5_003402"
     CDS             12607..13119
                     /gene="gspM"
                     /locus_tag="ACHKG5_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP0869438.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13240..14010
                     /locus_tag="ACHKG5_003403"
     CDS             13240..14010
                     /locus_tag="ACHKG5_003403"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0869439.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14102..14503
                     /gene="eptO"
                     /locus_tag="ACHKG5_003404"
                     /gene_synonym="gspS"
     CDS             14102..14503
                     /gene="eptO"
                     /locus_tag="ACHKG5_003404"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFP0869440.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14682..15853
                     /locus_tag="ACHKG5_003405"
     CDS             join(14682..14931,14931..15853)
                     /locus_tag="ACHKG5_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0869441.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            16394..16909
                     /gene="ehxC"
                     /locus_tag="ACHKG5_003406"
                     /gene_synonym="hlyC"
     CDS             16394..16909
                     /gene="ehxC"
                     /locus_tag="ACHKG5_003406"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP0869442.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
BASE COUNT         4232 a         3668 c         4749 g         4263 t
ORIGIN      
        1 atatttacga aacaggtgta aatatgttat aaaaataacc aacgactagt gaataagtcg
       61 ctcctgaaaa aataaaatat agaaatactg ttatatccgg ctgcatgaac actaaaatga
      121 atgagagatg gagaacaccg atgaaattaa agtatctgtc atgtacgatc cttgcccctc
      181 tggcgattgg ggtattttct gcaacagctg ctgataataa ttcagccatt tatttcaata
      241 cctcccagcc tataaatgat ctgcagggtt cgttggccgc agaggtgaaa tttgcacaaa
      301 gccagatttt acccgcccat cctaaagaag gggatagtca accacatctg accagcctgc
      361 ggaaaagtct gctgcttgtc cgtccggtga aagctgatga taaaacacct gttcaggtgg
      421 aagcccgcga tgataataat aaaattctcg gtacgttaac cctttatcct ccttcatcac
      481 taccggatac aatctaccat ctggatggtg ttccggaagg tggtatcgat ttcacacctc
      541 ataatggaac gaaaaagatc attaatacgg tggctgaagt aaacaaactc agtgatgcca
      601 gcgggagttc tattcatagc catctaacaa ataatgcact ggtggagatc catactgcaa
      661 atggtcgttg ggtaagagac atttatctgc cgcagggacc cgaccttgaa ggtaagatgg
      721 ttcgctttgt ttcgtctgca ggctatagtt caacggtttt ttatggtgat cgaaaagtca
      781 cactctcggt gggtaacact cttctgttca aatatgtaaa tggtcagtgg ttccgctccg
      841 gtgaactgga gaataatcga atcacttatg ctcagcatat ttggagtgct gaactgcctg
      901 cgcactggat cgtgcctggt ttaaacttgg tgattaaaca gggcaatctg agcggtcgcc
      961 taaatgatat caagattgga gcaccgggtg agctgttgtt gcatacaatt gatatcggga
     1021 tgttgaccac tccccgggat cgctttgatt ttgccaaaga caaagaagca catagggaat
     1081 atttccagac cattcctgta agtcgtatga ttgttaataa ttatgcgcct ctacacctaa
     1141 aggaagttat gttaccaacc ggagagttat tgacagatat ggatccagga aatggtgggt
     1201 ggcatagtgg tacaatgcgt caaagaatag gtaaagaatt ggtttcgcat ggcattgata
     1261 atgctaacta tggtttaaat agtaccgcag gcttagggga gaatagtcat ccatatgtag
     1321 ttgcgcaatt agcggcacat aatagccgcg gtaattatgc taatggcatc caggttcatg
     1381 gtggctccgg aggtggggga attgttactt tagattccac attggggaat gagttcagtc
     1441 atgaagttgg tcataattat ggtcttggtc attatgtaga tggtttcaag ggttctgtac
     1501 atcgtagtgc agaaaataac aactcaactt ggggatggga tggtgataaa aaacggttta
     1561 ttcctaactt ttatccgtct caaacaaatg aaaagagttg tctgaataat cagtgtcaag
     1621 aaccgtttga tggacacaaa tttggttttg acgccatggc gggaggcagc cctttctctg
     1681 ctgcaaaccg tttcacaatg tatactccga attcatcggc tatcatccag cgtttttttg
     1741 aaaataaagc tgtgttcgat agccgttcct ccaccggctt cagcaagtgg aatgcagata
     1801 cgcaggaaat ggaaccgtat gaacacacca ttgaccgtgc ggagcagatt acggcttcag
     1861 tcaatgagct aagtgaaagc aaaatggctg agctgatggc agagtacgct gtcgtcaaag
     1921 tgcatatgtg gaacggtaac tggacaagaa acatctatat ccctacagcc tccgcagata
     1981 atagaggcag tatcctgacc atcaaccatg aggccggtta taatagttat ctgtttataa
     2041 atggtgacga aaaggtcgtt tcccaggggt ataaaaagag ctttgtttcc gatggtcagt
     2101 tctggaaaga acgtgatgtg gttgatactc gtgaagcgcg taagccagag cagtttggtg
     2161 ttcctgtgac gaccctggtg gggtattacg atccggaagg cacgctgtca agctacatct
     2221 atcctgcgat gtatggtgcc tatggcttca cttattccga tgatagtcag aatctatccg
     2281 ataacgactg ccagctgcag gtggatacga aagaagggca gttgcgattc agactggcta
     2341 atcaccgggc taacaacact gtaatgaata agttccatat taacgtgcca acagaaagtc
     2401 agcccacaca ggccacattg gtttgcaata acaagatact ggataccaaa tcgctcacac
     2461 ctgcgccaga aggacttacc tatactgtaa atgggcaggc acttccagca aaagaaaacg
     2521 agggatgcat cgtgtccgtg aattcaggta aacgttactg tttgccggtt ggtcaacggt
     2581 caggatatag ccttcctgac tggattgttg ggcaggaagt ctatgtcgac agcggggcta
     2641 aagcgaaagt gctgctttct gactgggata acctgtccta taacaggatt ggtgagtttg
     2701 taggtaatgt gaacccagct gatatgaaaa aagttaaagc ctggaacgga cagtatttgg
     2761 acttcagtaa acctaggtca atgagggttg tatataaata atcctgagac ggtgcggcca
     2821 cttcggccgc attagtaaca cattcgtccc gttaatcaaa acataaagta gcgtccagcg
     2881 ttttattatg ttgttctttc tatcattccg gggtgaccgg ggcttattta taaaagatat
     2941 cgtacttaaa atgctgacgc caaaccggct attgtgcgta attttactta ttgccggata
     3001 tcagctggtg tcggttatcc atcatttctg gctgactcag gcagcatcgg tgcccggctt
     3061 atcccgtgtt agcgcgccgg aaacagcggt aactggtgat caaactgaag aacgttttgt
     3121 ttttacgtta ttcggcaggg catccccact atcatcggag gggagagcgc aggaaacaat
     3181 gccttccctg tcagatgatc tgctttcagg ggaggatctt gacgtgagag gtatacttta
     3241 tagttcggtt gcagagcatt ccgttgccat atttgcacat aataacagac agttcagtct
     3301 gagcgtcggc gaaaaagtac ccagctacga tgctacgatt agtgctattt ttagcgatca
     3361 tattgttatt aactatcagg gaaaaactgt atcactgcct ctgcgatatg ataataccga
     3421 aaaaaagaat gcatatgaca ataataattt aacagttgga gacgtgataa cccaagataa
     3481 ttttcgggta gaaagtgttt ttgatattat gagcttttca gccgttacgg ttaataatac
     3541 attaagcggt tatcgcttga ttccgggtaa gcacagttcg ttattttata atgctgggtt
     3601 gcatgataac gatctggccg tatcggttaa tggttcagaa ttgcgtgata ccagacaggc
     3661 gcagcagata atgaagcaat tgccagaact taaagaaata aaaataaccg tcgagcgtga
     3721 tggtcagtta tatgatgcat ttattgctgt aggagaaaac tgattgttca ggaaatggtt
     3781 gaatgggcgt ttgccggtac ttgtgttcac tacagtaatt ttgggggcca ttccagggtg
     3841 gggggctgaa ttttcggcca actttaaaga tacggatatt caggagttca taaatactgt
     3901 cagtaaaaat ttacacaaaa cggtaataat taatcctgac gtgcagggaa ccatcactgt
     3961 acgcagctac gatatgctga acgaggaaca atattatcag ttctttctca gtgtgctgga
     4021 cgtttatggt tttgctgtgg tcgatatgca caacggtata ctgaaagtag tgcgctcaaa
     4081 agatgccaaa acgtcggcgg tgccggtagc tagtgatgtc agtcccggga ctggtgatga
     4141 ggttgttacc cgggtggtcc ccgtaagtaa cgtggcagcc agagatctgg cgcctttgct
     4201 gcgtcagctc aatgataatg ctggcgcagg aagcgtggtg cattatgaac cttctaatgt
     4261 tttgttgatg accggacgtg ctgcagtgat gaaacggttg atggagattg ttgaacgtgt
     4321 ggataaggtg ggtaatcgca gcgttgccac ggtcccgctc acctacgcgt ccgcaacaga
     4381 cgtagccaga cttgttacgg aactgactaa agaaacagat aagacagcta tacctgcttg
     4441 gatgacggcg aaactggttg cagacgagag gacaaactca gtgctcgtca gcggagagcc
     4501 aatctcccaa cagcgtatca tctccataat taagcaactg gatcgtcagg aggatgttca
     4561 gggtaatact aaggtgattt acctgaaata tgcgaaggcg aaggatttag tggaagtcct
     4621 gacaggtatc agcagcagta ttgaaaacga ctctaaaaag agtccgtcaa cggaagcctt
     4681 gcgcaaagga gtgacgatta aatcccacga acaaaccaat gccctgatcc tgacgggggc
     4741 ccctgacgtc atccgcgacc ttgaaaatgt gatttcgcag ttggatattc gtcgtcctca
     4801 ggtcctggtg gaggccatca ttgctgaaat acaggatgct gacgggctga accttgggat
     4861 ccagtgggtg aataaacatg ccggtgtggc gcagttcacc agtaccggtt tacctattac
     4921 cacgatggtt cagactcgtc agaacgaaat cttagacagc gatcagagca atgccctgag
     4981 catgtttaac ggaattgcag cggggtttta tcagggaaac tgggcgatgc tgttgacggc
     5041 gctctccaca agtagcaaga atgatatctt ggcgaccccc agtattgtca cgctggacaa
     5101 tatggaggcc actttcaatg ttggtcagga ggtcccggta ctttcgggct cacagacaac
     5161 ctctggggac aatattttta acacggtcga gcgcaaaacg gtggggatca aactcagggt
     5221 aaaaccccag atcaacgagg gtgattccgt gttactggag atagaacagg aggtgtccgg
     5281 tgtggcggac actgcagtag ccaccactac tgacttggga gcaaccttca acacccgaac
     5341 agtgaccaat gccatgctgg tcgggaatgg cgaaacggtg gtggtcggag gattactgga
     5401 taagtcgatc agggggagtg agagtaaagt gccactgctg ggggatatcc cggtactggg
     5461 gcatcttttt cgcgcaaaaa gcgaacagac agctaagcgt aatctgatgc tgttcattcg
     5521 gccaactatt attcgtgagc gcgacggatt tcgtcatgct tcggccgaaa aataccagtc
     5581 gtttaatcag gaacaggtgc agtcgcgtgg caaagaaaca acggcgctga cgctgaatga
     5641 ggaacagctc aggctgtccc ccgatcaaga cgatacggct ttccggaagg tgaaagcggc
     5701 gattgctgcg ttttatgcgc aggagatgta atgagcagag tagtacagaa tgtcagtgaa
     5761 tcacgtcccc ttctgccttt ttccttttcc cgtacgcaga gaattttgtt gctgcgtgaa
     5821 caggagggga atcgagtgtt ctgcatggag gacacacctg ccagtgccct gttggaggtg
     5881 cgcagggtgg ctgaaggtcc tctgaatgtc acaacagtgt cggcggaagc gtttgaaaaa
     5941 cagctggtga gcagctatca acgtgattct gatgaagcgc gacaaatgat ggctgagatt
     6001 ggtaatgaaa tggattttta taccgtcgca ggggagttgc ctgaccgtga ggacttactg
     6061 gatgcgaatg acgatgcgcc aattatccgc ctgattaacg caatgctgac agaggcaatt
     6121 aaagagaaag cctcagatat tcatattgaa acttacgaac gccatctgca ggttcgcttt
     6181 cgcattgatg gcgttctgcg ggagatcctc cgtttacata ggaatctggc ttcgttactg
     6241 atttcacgca ttaaagtcat ggcccggttg gatattgccg aaaaacgcgt tccccaagat
     6301 ggccgcatgg tactgcgtat cggtggtcgg gcggtggatg tgcgtgtttc aacgttgcct
     6361 tcaaatcatg gtgaacgcat cgtgctgcgt ttactagaca aaaatagcgt tagtctcgat
     6421 cttgctgcac tcggcatgtc gcagcagaat cagcgacaca ttgatgcact gatccgccgt
     6481 cctcacggca ttattttggt gaccgggcct acagggtcgg gtaaaagcac gacgctttat
     6541 gccgcgttaa gcctgctgaa tccccgagac cgcaacatta tgacggtcga ggatcctgtt
     6601 gaatatgaac tggacggtat cagtcagacc caggtcaacc cgaaggtgga catgaccttt
     6661 gcccggagtc tgcgcgctat tttacgtcag gatccggacg tggtgcttgt gggggagatc
     6721 cgtgacggtg aaacagccca gattgctgtg caggcctcgc tgacagggca tttggtgctg
     6781 tcaacgttac atacgaatag tgcagcaggc gcgctgtcgc gtctgcagga tatgggcatc
     6841 cctccttttc tgctttccac ctcattactg gctgttctgg cccagcgact ggttcgcacg
     6901 ttgtgtccgc gctgtcgtca gccctgtcag gtcagtactg agttagcgat ggacatggac
     6961 attccccctg aaaccacgat ctggcagcct gccgggtgtc agcactgcag tttcacgggc
     7021 tatcacgggc gcaccgggat ccacgagctg ttgctgattg acgatcgcat tcggacggca
     7081 atctatcagg gggaggggga gctgggtatt acccgcttgg cagggagtcg ctatctgacg
     7141 ctgcgtggcg acgggcgaca aaaggtacta gccggtgaga ccagttggga ggaggtggtt
     7201 cgcgttactg aaagcagatt gcaggaagag gaatgatatg gcgttgtttc attatcaggc
     7261 atcagatatt cacggtagaa aacgcagtgg cattctggag gcggattcgg cccgacatgc
     7321 ccgtcagttg ctgcgtgagc aggcgcttat tccagtcagg ttggatgaga aacaggtcca
     7381 tcacaagcac tcactgcgga gtatcctgag gtttcgtccg cgcgggggga gcagcgccga
     7441 actcgcgtta ctgacacggc agttagccac actagtggcc gcatctctgc cgcttgaaga
     7501 ggcgctggat gcgttgttga ggcagagtga aaaaccgcgt cagcggaact taatcgctgc
     7561 cgtgcgcacc aaggttctcg agggacattc cttggccgcg gcgatgggca tgtttccagg
     7621 tacgtttgag cgtctttatt gtgcgatggt ggcggcgggg gaaacatccg gtcgtttgga
     7681 cgtggtgctc agtcgtctgg ctgactatac cgagcagcgc cagatcatgc gaaaccgtct
     7741 actccaggca ttgctctatc cttgtgttct gacgttggtg gcggttgggg ttattgccat
     7801 tctgcttact gcggttgtgc cgaaagtggt tgagcagttt attcatatga aacagaccct
     7861 tcccttatct acccgcgtac tgatgggggc tgctgaggtg agccagactt ggggcccgtg
     7921 gctgttactg gccgcagcgc tgggcgggat agcggggcgc atgatattac atcagccctc
     7981 ccagcgtctg gcttttcatc atctgctgtt gcggctgccg gtcgtggggc gcatatcgcg
     8041 cggtctgaat accgcacgtt atgcccgcac gctgagcatt cttaatgcca gtgcggtacc
     8101 gctgctccag gcgatgcaca tcagtggtga cgtactgagt aatgactggg cccgtcatca
     8161 gttagctacc gcggccgagt tggttaggga aggggtcagc ctgcatcagg cactggagca
     8221 gacttcgctg tttccgccta tgatgcggca catgattgcc agcggtgaaa atagtggcga
     8281 actcgacagc atgttggaac gggccgccga caatcaggat cgtgagttca gcacacagat
     8341 gcaactggcg ctgggattgt ttgaaccgct gttggtggtg ggtatggccg gggtcgtttt
     8401 gtttattgtt ctggcaattc tgcagccgct cctgcagctc aacaacatga tgaatatgtg
     8461 aatccttctt taacagtcaa cggagaaaaa catgcgaaaa cagcatcagc gtggttttac
     8521 cttgctggaa ataatggtgg taattgtgat cttgggagtt ttggccagtc tggtggtccc
     8581 gaatctgatg ggaaataagg ataaggccga tcggcagaaa gtgatgagcg acttggttgc
     8641 gctggaaagc acgctcgata tgtaccggtt agataataac cggtatccga caacggagca
     8701 gggactgcgg gcactggtca gtaaacccac cgtgcaacct gaaccccgta actaccggca
     8761 ggatggttat atccgccgtt tgccccagga tccttggggg ggggattatc agctgttaaa
     8821 tcctggtcag tacagcgaca ttgatatctt ttctcctggc ccggatggtg tgccaaacac
     8881 tgaagatgat attggtaact ggacgctcgg taacgcgcag ccatgagtca gcgcggtttc
     8941 acaatactgg agatgatgct gatccttttg ttgatgggca tcactgcagg gctggtacta
     9001 atgtcatttc ctgactctgc tcagaatcat ctgcaacaac agcgggagcg tctgcaggcg
     9061 cagctggatt atgccttgga tagaagccag caggacggct tgctgatggg gattcaggtt
     9121 cggtttgacg gatggaaatt taaggttctg cagcgcggaa cagcggaaag ccccccgaca
     9181 ttagctgagg gaggggatat ttggcagggg tatgtctggc aaacttggca accgcgacgt
     9241 gcagccatgg ggggcaaact tccggatgat gtgcgtcttg agctgcagta tctcagaggt
     9301 ttgcagtgga tgtctgagca tgatgacggg gctgaacccg atattttgct gcttcccgga
     9361 ggtgaggtaa cgccgttcag acttcttttc aggcaggtcg gagaggaggc cgttgtcggg
     9421 ctgcaggttg atgagaacgg tttgatgact ctttttgaag gtgaggtctc attgtgaagg
     9481 ggcaagaagg aatgacatta ctggaagtca tagtagctct ggttgtgttt gcgctggcag
     9541 gaatggcgct gatgcaggcc tcgacccagc aggctgcggg gatcggacgg atggaagaga
     9601 aggtgctggc aggctggttg gccgataacc agatggtgca attgcaactc gagaaaactt
     9661 ggccggaaaa tggctggggc gagaagacga tcagcttcgc tggcaccgaa tggtatttac
     9721 gctggggcgg gccggacagc gacgtgccgc agccgcgctc cttagaggta gaggtacggc
     9781 gcactaaaga ggagacgtcc gccttggttt ccttgcgtag cagtgtggtt cgtgaatgag
     9841 tcagcaacgg gtaaaaggtt ttacgctact ggaaatgctg ttggcactgg ctgtttttgc
     9901 agcgttgagc atcagcgcat ttcaggtgct gcagggtgga atacgggcgc acgagctgtc
     9961 ccgggataaa gtacagcgtc tggctgagct gcagagaggg atcagccaga tggagcgaga
    10021 tcttacgcag atgctcccaa ggcacagccg ggggaatgaa ggcctgctgc tcgctgcacc
    10081 tcatctgcta aaaagtgatg actgggggat ttcttttacg cgaaacagct ggctgaatcc
    10141 cgccgggatg ctgccgcgcc cggagttaca gtgggtaggt tatcggttgc gacagcaaaa
    10201 gttagaacgc ctgagctact ttcatgtcga tcacccttcc ggtgtttctc cggatgtcag
    10261 ggtgatgctg gacggcgttc acgcatttcg tctgcgtttt tttgttaacg gtgattggca
    10321 ggcgcgctgg gacagtaccg gcatcctgcc gcaggctgtt gaagtgacgc tggttatgga
    10381 tgattttgct gaactgcccc gtctgtttct tgtgagcaag gagaccgctg aatgaaattg
    10441 cgggaacagg gcgtggcgct tctggtggta ttgctgatcc tgtcgttaat ggtaacgata
    10501 gcggcggtga ttgcggagcg caacggccgg acatttttgc gtacagtagc gcagttggat
    10561 cagctgcagg caaagtggga tggctatacg gcagagacga tagcaaaaca aatcttacag
    10621 aggagcaggc aggagtcgcc ccggaaaacg catctggcac aaaattgggc gcagtcagaa
    10681 cgacagtttg agaccagagg gggagatgtg cgggggcaga tcgtggatgc gcaggcctgt
    10741 tttaatctta atgctattaa ttatggagtg gtggatctga ccagtatacc ttatgccgcc
    10801 agaatttttc agcagctatt aatcaacttg caggtagagc ttttacaggc ccggcaagtg
    10861 acggcagccc tacgtgactg gatcgatcgt gatgataagc cggtaagggg cggtgctgaa
    10921 gacgaggtgt atatggggat ggaacctccc tatctggcag ccaaccagcc gatgcaggac
    10981 gtcagtgagt tacgccttat ccggggaata gatgccaggc tttaccgcaa acttttgccg
    11041 tatgtttgtg tactgcctac gtcagatctg tctgtgaatg tgaatactct gctggattca
    11101 caggcgccgc tgctggcggc gctctttctg acgaaaccgg acagtcttcc ggtgacggaa
    11161 ttactgcagc ggcgaccccg tactggttgg gagagcgtgg cggcgtttct tgacgcgcca
    11221 ccgcttaagg acattgacac cagtgcggcc atgccggtat tggccgtcag cagcaactat
    11281 tttctggttc ggttacatgt gcgctccggg gaacacctgt tcagtcagca gactttaatg
    11341 cagtggaggg aggagcgttt ccggatcatt cagcgacagt atggattaac gatgagggag
    11401 gttccctgaa aaaaaataac aaacatgtcg aggctggtcg gctttttctg cgactgccgg
    11461 aggatgactc ggagccttta ttgtggaggc tgattgcgcc agaggagaca ggagccggca
    11521 gtgtggcagt cggagaaacg gatccgcagt tagtcggttt gctgtcccgt tatcccgcct
    11581 gggtgctggt tccgtcaggc agaattgcct tccatcaggt cacgctgcca caccgcacgt
    11641 ggcgtcagcg tttgcaggtg ctgccctttc ttctggaaga gcagttggcg acagacattg
    11701 agcagctgca cttcgccatt cttcatcagt cgggtgacag gtgtgatgtg gcggtggtcg
    11761 aaaaagcggt tatgcatcgc tggttagcgt actgtggaca acttggggtt cgggaggtga
    11821 ggttgctgcc tgacgtactg atgctgccgt tggcaactga aggatggagt gccgtaaagc
    11881 ttgccgggca gtggctgttt cgccgggatc gttacgccgg gatgacggta gagttatcgt
    11941 ggctgacaca tttcttgtct cttactgcgc cgccggtgat tgagtcctac tccgtgccgc
    12001 cagtaccgtg cccgggtccg aagaccactg agtggcgaaa tcagcccgga cgcgatctgc
    12061 tgcagttggt ggccgaaggt gacgggtatg atggcgcaga tctgcggcag ggagaatttg
    12121 cccgccaggg aacatggtat gcacgatttc gcccatggag gtatgtcgca ggggctctgc
    12181 tggcctgtgt ttttctggcc gggagcaacg cggggctggc acattatcgg ctctggcagc
    12241 aggctcaatt ctggcggtat gagagcgtac gtgtctatca gcagctgttt ccgagtgaaa
    12301 aggcggtgaa ggatccccgc cggcagatgc tgagacatct gcaacagtcg acgggtaaca
    12361 atataccgga actcgggagc gtgatgcgcc agcttcagca actgctgtca gaaacctcaa
    12421 cgatacgctt acaggcactc gcctgggaca gcagcagcca gacactgaaa attgatttgc
    12481 aggcggcttc ttttcaggcg ctggaacact ttcagcagat cgccggagaa aaatacctca
    12541 tccagcctgg tgagatacga cagcagccgc agggggtaga aagtcgtctg atcctgagag
    12601 tgaacaatga acgagcttaa aaaacgtttg cagtatgtta gcccccgtga gcgcaggctg
    12661 cttatcggat gtgttgcctt actggttgtt gtttttgtct attacgtcct ctggcagccc
    12721 tggctgatac gagaggataa gtggcggact gtagttaccc gtgaaaaaaa cacagtggag
    12781 tggatgaaac aacaggtacc caacataaag agacgggagg caagtatgcc tgaacagggg
    12841 gagccgttag gtttatctgc ggccgtgaca cgtacttcag ccgcatacgg tgtatcggtc
    12901 acccggttgc agcctcaagg ggagcgtctg gccgtcacct tagccccggt tgaatttaat
    12961 gcgctgatgc agtggcttac gcaactggag cgtcggcacc gtattcgtac catggtattt
    13021 gatgtggccg cgcagggcaa cccacccgga caggtaacgg ttaatcggct ggttttgtcc
    13081 caagatgacg taaacaagga cgcttcgctt agtcgctgat gtcgtaattt acagattaat
    13141 gcttgatgta aaaattggac ttataaagga ggatgtgtga gtagtttgcc ggaatttgcg
    13201 gttgattatc ccttgttgtg gctcatctgc accggagggc tggggggcgt taccggcagt
    13261 tttcttaacg tcgtgattca tcgtctgccg ataatgttgg agaggcggtg gcggcgtgaa
    13321 gcgctgacgc tattggcaca ttctgtgcct gagtccgggc cggcctttaa cctgatgata
    13381 ccgcagtctc attgtccctg ctgttttcat ccgctcggca tcagggataa cattcccctg
    13441 ttaagctacc tatttctgaa ggggaaatca cgctgttgtg gcgagcgtat tcccgtttac
    13501 tacccgctag tcgaaataac gaactcagtg ttattcattc tggccgcgag caggtttcca
    13561 ccagggctga cattagcggc tgcctggctt tttatttcca tgctgttggt tctggcggtt
    13621 attgactgcc atacggcgct gctgccggat gtgctgacgt tgccattgct ctggcttggc
    13681 ctgttattca gtttacagag aggcgttgtc accttagagg aggcggtagt gggggcggta
    13741 tctgggtatc tctgtctgtg gggactgtac tggctttttc gtttcgctac cgggagggaa
    13801 gggttaggtt acggtgattt taagctagct gcggcattgg gagcatggat gggttggcag
    13861 gcattaccct caatactttt gtttgcctca gtcagtgggt taatcgtaac agtactgttg
    13921 cgtattgtga cagctacgga ctttacccgc ccgctgcctt ttgggccgtg gctcgctttg
    13981 agcggagtgt gccatttttt gttgatgtag cgttcggtta ccggggctag taataaaaac
    14041 ggctcagcgg ataaaagaac tgttgtaaac cggtatcagg ttaaacgata cctagtaaaa
    14101 aatgatgggt aatattttga aaaaactgaa ttgtatcgcg tcattactag tcttagtgac
    14161 tatctctggt tgtcatcagt caccctccat ccataagcag gccactgttc ctccgagcga
    14221 acaactggaa caaatggcgt cgattgtctc tgcgacacgg tatctgaaaa tgagatgtaa
    14281 ccgtagtgat ttaccagatg aacagtcgat cctaaatgtc gctaaccgga tcgctatcgg
    14341 gaaagggtgg cagtcattaa ctcaggagga tatacgtaaa cacagcgatg atatctatgt
    14401 gcgtctgact agggacagta cgcctgagta tataaaatgc agagaattca atcggcggct
    14461 ggtcccattt atcggtgaat tattggcaag gggtcgtgga taatatgaac cgccccggaa
    14521 atcctggaga ctaaactccc tgagaaagag gtaaacagca tgaataaaaa tgggtcttcc
    14581 cttgtagtgg tggctgaagg catgatgatt gtagtggcac actgaatttg gccacctgaa
    14641 cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa agaaatttca
    14701 gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaactac acggtggcag
    14761 atgccgccaa agctatggat atcggccttt ccacaatgac aagatgggtc aaacaactgc
    14821 gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa caaatcgaaa
    14881 tacgtgagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg
    14941 ctacaaccgc gctcttgatg tcagactccc tgaacagttc tcgataatcg ggaaactcag
    15001 agcgcattat cctgtggtca cactctgcca tgtgttcggg gttcatcgca gcagctacag
    15061 atactggaaa aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagtcaggt
    15121 acttgagttg cataacatca gccatggttc tgccggggca agaagcatcg ccacaatggc
    15181 aaccctgaga ggcttcagaa tggggcgctg gcttgccggc aggctcatga aagaactggg
    15241 actggtcagt tgccagcagc ctgcgcaccg ttataaacga ggtggtcgtg aacatgtcac
    15301 tatcccgaat caccttgggc ggcagttcgc agtgacagag ccaaatcagg tatggtgcgg
    15361 cgacgtgacg tacatctgga cggggaaacg ttgggcatac cttgccgttg ttctcgacct
    15421 gtttgcaagg aaaccggtag gttgggcaat gtcgttctct ccggacagca gactgaccat
    15481 caaagcgctg aaaatggcct gggaaatccg cagtaaacca gccggggtaa tgttccacag
    15541 cgatcagggc agccactata caagcaggca gttccggcag ttactgtggc gttaccagat
    15601 caaacagagt ctgagtcgac gaggaaattg ctgggataac agcccgatgg agcgcttctt
    15661 caggagtctg aaaaacgagt ggataccggt gacgggttac atgaacttca gcgatgctgc
    15721 ccatgaaata acggactata tcgttgggta ttacaacgcg ctcaggccgc acgaatataa
    15781 cggtgggttg ccaccaaatg aatcggaaaa ccgatactgg aaaaactcta aagcggtggc
    15841 cagtttttgt tgaccactac atcccgtcag ccgatttacg ggccgtaagt aacgaagttg
    15901 gatgcaaatg tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg
    15961 cgcatttgaa aaaactccag ctataccgga agagatttat tattaacagg agaggtttgc
    16021 ttttctgtgt aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat
    16081 ttcggtaact gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc
    16141 acgtggctat tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata
    16201 atataactta gctgtgatat gtgtaagaat gtttaggcaa tatattatta tcgctaatgt
    16261 tgttttggtt gggagagatt ctccttgcgg tagctttctg tttttagatg cttcttgctt
    16321 aaaaggatat aatttctgtt cttttatata gggttcttta catttactga gttttcttat
    16381 ttatttaaac attatgaaat ctaatgcttt tgacgtcatg gggaaggttg catggctatg
    16441 ggcctgttct cctctgcata aaaaatggcc attatctgtt tttgctatta atgtcatacc
    16501 tgctattcag acaaatcaat ttgctctctt aataaaggat gagcttcctg tagctttttg
    16561 tagctgggcc agtttagatc tggaatgtga ggtaaaatat ataaatgatg taacatcttt
    16621 gtatgcaaaa gactggatgt cgggagaaag aaagtggttt attgactgga tagctccatt
    16681 tggacacaac atggagttat ataaatacat gaggaaaaag tatccttatg agttattcag
    16741 agcaataagg ttagatgaga gcagtaaaac aggaaaaata gctgaatttc atggaggggg
    16801 gatagataaa aaactggcat caaaaatatt caggcaatac catcatgaac ttatgtctga
    16861 agtaaaaaac agacaagatt ttaattttaa tattgagaaa gaaaactaat at
//
LOCUS       DAZGXS010000100        16605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGXS010000100 DAZGXS010000000
VERSION     DAZGXS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..113)
                     /locus_tag="ACHKG5_003407"
     CDS             complement(<1..113)
                     /locus_tag="ACHKG5_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869443.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSST"
     gene            complement(260..1543)
                     /gene="yeaH"
                     /locus_tag="ACHKG5_003408"
     CDS             complement(260..1543)
                     /gene="yeaH"
                     /locus_tag="ACHKG5_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0869444.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1656..3590)
                     /gene="yeaG"
                     /locus_tag="ACHKG5_003409"
     CDS             complement(1656..3590)
                     /gene="yeaG"
                     /locus_tag="ACHKG5_003409"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0869445.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4026..4772
                     /gene="mipA"
                     /locus_tag="ACHKG5_003410"
     CDS             4026..4772
                     /gene="mipA"
                     /locus_tag="ACHKG5_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0869446.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4862..5716
                     /gene="yeaE"
                     /locus_tag="ACHKG5_003411"
     CDS             4862..5716
                     /gene="yeaE"
                     /locus_tag="ACHKG5_003411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0869447.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5767..6651)
                     /gene="yeaD"
                     /locus_tag="ACHKG5_003412"
     CDS             complement(5767..6651)
                     /gene="yeaD"
                     /locus_tag="ACHKG5_003412"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0869448.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6735..7730)
                     /gene="gapA"
                     /locus_tag="ACHKG5_003413"
     CDS             complement(6735..7730)
                     /gene="gapA"
                     /locus_tag="ACHKG5_003413"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0869449.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8072..8485
                     /gene="msrB"
                     /locus_tag="ACHKG5_003414"
     CDS             8072..8485
                     /gene="msrB"
                     /locus_tag="ACHKG5_003414"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0869450.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8527..8799
                     /gene="yeaC"
                     /locus_tag="ACHKG5_003415"
     CDS             8527..8799
                     /gene="yeaC"
                     /locus_tag="ACHKG5_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0869451.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9169..10245
                     /gene="ydjL"
                     /locus_tag="ACHKG5_003416"
     CDS             9169..10245
                     /gene="ydjL"
                     /locus_tag="ACHKG5_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0869452.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10272..11651
                     /gene="ydjK"
                     /locus_tag="ACHKG5_003417"
     CDS             10272..11651
                     /gene="ydjK"
                     /locus_tag="ACHKG5_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0869453.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11668..12711
                     /gene="ydjJ"
                     /locus_tag="ACHKG5_003418"
     CDS             11668..12711
                     /gene="ydjJ"
                     /locus_tag="ACHKG5_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP0869454.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12732..13568
                     /gene="ydjI"
                     /locus_tag="ACHKG5_003419"
     CDS             12732..13568
                     /gene="ydjI"
                     /locus_tag="ACHKG5_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP0869455.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13573..14505
                     /gene="ydjH"
                     /locus_tag="ACHKG5_003420"
     CDS             13573..14505
                     /gene="ydjH"
                     /locus_tag="ACHKG5_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0869456.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14515..15495
                     /gene="ydjG"
                     /locus_tag="ACHKG5_003421"
     CDS             14515..15495
                     /gene="ydjG"
                     /locus_tag="ACHKG5_003421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP0869457.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15632..16390
                     /gene="ydjF"
                     /locus_tag="ACHKG5_003422"
     CDS             15632..16390
                     /gene="ydjF"
                     /locus_tag="ACHKG5_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0869458.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16508..>16605
                     /locus_tag="ACHKG5_003423"
     CDS             16508..>16605
                     /locus_tag="ACHKG5_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869459.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLL"
BASE COUNT         4320 a         3724 c         4186 g         4375 t
ORIGIN      
        1 gtgctggatt ttattgatgg cagagatata tagttttgac caggatcaat tttcgttaac
       61 gataaaaaat atttaaagaa taaccccagg gaaatacgtg ttgacagaat aatttttccg
      121 aatgattgtt ttctggaact aaattgtgta aaggttatgc tgtcatattt aaagcgattg
      181 taagctaatg tatgtaataa atgagataac ttacagtgag ctaaaaatca gccaggcgat
      241 aatgacccgg ctgatagttt tagtctttag ctgttgcatt ttgcttatga aacagttcac
      301 ggaacaccgg ataaatatca tcctggtcgc ggatatgctg catcgcaaag ttgtcgaaag
      361 tagattgcag atgctcatat tctcgccaca gtgtctgatg cgcgcgacgg gtaatttcga
      421 tatagctgta ataacgaaca acgggtaata attttttcgc cagaatttca tggcaaagcg
      481 gagagtcatc ggcccagtta tcgccgtccg atgcttgtgc agcgtaaata ttccactgtg
      541 ccgggttata acgctctttc actacctcat ccatcagttt cagggcgctg gaaacaatgg
      601 tgccgccagt ttcctgcgag tagaaaaact catgttcatc gacttctttc gcctgggtat
      661 gatggcggat gtatacgact tccacgttct tatacgttct gctgaggaac agatacagca
      721 gaatataaaa acgcttagcc atatctttag tggattgatc cattgaaccg gaaacgtcca
      781 tcaggcaaaa catcactgcc tggctggagg gatcgggccg cttctcgtag ttcttgtaac
      841 gtaaatcgaa ggtgtcaata aaagggacgc gttcaatttt ggcacgtaat tctgcaattt
      901 ctttgcgcag acgttcctct tccagcagct gcgcaggttc actgttgctg atgatggcca
      961 aattctcttc cagtgcatga agttcccgcc gcttgcctgc cgtcatggct gtgcgtcgcg
     1021 ccagtgagtt ctgcaaggaa cgcacaacgc tgatattggc cggaacgccg ttagcggtat
     1081 aacccgcccg atgcgtttta tattcggtca gctggcgttg ttggttttgt ttcagattcg
     1141 gtaaggccaa atcttcaaag agcagatcaa gatactcatc tttcgaaatc tgaaagacaa
     1201 attcatcctg accttcacca tcctggctgg cctggccctg accactgccg gaacctccgc
     1261 cgccaccctg gggacgttca attcggtcgt tctgaacgaa atggtcattg cccggatgca
     1321 cgcggtggcg cagaccgcca cgcccctgat gaaacattgg ttcgctaata tcttccgtgg
     1381 gaatggatac ggactcgccg ctgtcgacgt cagtcaccga acgcttatta atggcctcgg
     1441 agatcgactg tttaatttgc gctttataac ggcgtaaaaa acgctggcga ttcaccatgc
     1501 ttttgttttt gccgttcaga cgccggtcaa taaaccaggt catatgcccc ccgtactaca
     1561 tttgccaact gtatgctacg cctgtgttgc catttctctg gcccacggtt cgctgtcgtt
     1621 gtaggcccgg tagcatgtgt accgggccag ggttgttaag acgatttacg tacgcgcaaa
     1681 taccattcgc acagtaaacg cacctgttta cgggtgtagc ctttttccat catacggtcg
     1741 acaaagtcgt cgtgtttctt ctgctcgtcg gttgacgttt tggcgttaaa cgagataacc
     1801 ggcaacagct cctcggtatt ggagaacatt ttcttctcga taaccgtgcg cagtttttca
     1861 tagctggtcc agttcggatt gcgtccgctg ttattggctc tggcgcgcag tacgaagtta
     1921 acaatctcgt tgcggaaatc ttttggatta ctgatccccg ccggtttctc gattttctcc
     1981 agctcggcgt taagagactc gcggtcaaac agctgcccgg tatccggatc gcgatactcc
     2041 tgatcctgaa tccagaaatc cgcgtaggta acataacggt cgaaaatgtt ttgcccatat
     2101 tcggaatagg attcaaggta ggccgtctgg atctctttgc caataaactc ggcatatttc
     2161 gggatcagat aacctttcag gaactccaga tagcgttctg cctgctcttg tgggaactgc
     2221 tcgcgctcga tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct
     2281 acatgatcga agttgaacac gcgggagagg atcttaaacg caaaacgcgt cgacagaccg
     2341 ttcatacctt catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttggga
     2401 tcggtgtctt tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt
     2461 tctggctctt tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag agtgccaggg
     2521 gcgcatgggg cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct
     2581 tcggaaatgc gcaagcaata cggcaccttc acgatgtaaa cacgatcgag gaaggcttcg
     2641 ttgtttttgt tattacggaa agtgacccat tcggactcgt tcgagtgggc aagaataatc
     2701 ccgttgaacg gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg
     2761 gttaacaagg gatgcagcac ttttatcggt gctttaaaca tctcgacaaa ttccatgatc
     2821 ccttggttgg cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg
     2881 taatgttcga gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc
     2941 tcatcaccgg gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc
     3001 actacccgga acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccacggc
     3061 gacatgatgg tgccgagata acggcgagga atgccatact ctttttccag aatctgcgca
     3121 tcttcctgcg gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg
     3181 gcgctcaata cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat
     3241 gatttacccc cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc
     3301 tgagccgcgt gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac
     3361 tcttcaaacg ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggt
     3421 tcctgggctg tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg
     3481 ttggcataag cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct
     3541 tcgtccttgg cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt
     3601 cctttcgttt tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag
     3661 aggtaaactg aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg
     3721 atgagaacaa cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa
     3781 cttgcctaca cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc
     3841 ataattaatg taaagaccag gctcgccagt aacgctaaat tcatttggct gtaagcgcgg
     3901 tgtcatccgc gtcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg
     3961 ggtttcgatg tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat
     4021 taattgtgac caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg
     4081 tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gaacacccat
     4141 ataaagatta cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact
     4201 tctggttccg tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt
     4261 caattaccgc ttactggtcg ccgctttact tcaaagctaa agacagtggc gatcaccaaa
     4321 tgcgtcacct ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta
     4381 cccagtacgg ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca
     4441 tcgtctggga tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg
     4501 gtattggtgt gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca
     4561 aagagtccgc tcgcagcggt ctgcgtggct ataactcgaa cgacagctgg agcccttacc
     4621 tggagctgag cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct
     4681 acacccgtct gtctgatgaa gttactgaca gcccgattgt ggataaatcc tggactggcc
     4741 tgatttctac cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc
     4801 ataaaaacag ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac
     4861 aatgcaacaa aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg
     4921 aacatggtat atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc
     4981 gggcattgaa ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc
     5041 cgaaaaggtg gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa
     5101 agtctatccg tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg
     5161 ccgtctcaat actgattatc tcgatctcta cttattacac tggtctggca gtttcgcttt
     5221 tgaagagact gttgcagtga tggaaaaatt gatcgcccag ggaaaaatcc gccgctgggg
     5281 cgtttcgaac cttgattatg ctgatatgca ggaactctgg cagctgccgg ggggaaatca
     5341 gtgtgccact aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact
     5401 cccctggtgc cagcaacagc agatgccggt gatggcttac agtccgttag cccaggccgg
     5461 gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgctcacaa
     5521 tatcagcgcg gcgcaagtat tgttggcgtg ggtaatcagc catcagggtg tgatggcgat
     5581 tccaaaagcg gccacgattg cccatgtcca acagaatgcg gctgtgcttg aggtcgaact
     5641 ttcgtcagcg gaattagcca tgctggacaa agcgtatccg gcaccaaaag gaaaaactgc
     5701 gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac acaacattga
     5761 cgcaaattaa cgtttggcaa cgcgaatgga ttgcgccaga tgtgcaggtt tttctttggt
     5821 cactttttgc atttctgagg cgtaagctgt ttctacacaa acaaaggtct tgtagccatc
     5881 gtccggcatg tcgcccatgc taactgaaag cgccggtccc gggttccagc ctacaacgtt
     5941 cagatgatgc tggtggccta cggcgataat acgattcagc gcttcatcat taatcacgct
     6001 gcaatcttgc ggattcagat acacgcgatc ggtacggtca gggaaggtct gaataccgtc
     6061 ggtcagtaca tcttctttcg cgtcatttac tttatcaatg aagcgatcgc ccagcccact
     6121 gacgcttacc ttagcgatat cacccacgtt aaagtaggta tgcagggcag aggtggtttc
     6181 aaattcgccg tgtgcttcaa gatcgatttc acagttttta tccacgcgga aatgcgctaa
     6241 cagagtaaag tcgtgcagcc agaatttttt cgtctcttcg ctttgtgtca attcaaaagt
     6301 cagcgctacg ccattagcat cttcacgatg tgatttcagc gtccacggca ggttgcgggc
     6361 aaaaccgtgc gcaggcaggc cttgttgtgc cgccggacca aaccacggcc agcaaaccgg
     6421 tacgccaccg cgaatagcaa cgccattttt gaacggtgtg ttgttgctca accacagaac
     6481 ttcttcttca cctgcaggtt tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc
     6541 ttttacctgg ggatgatcga ctacaatgag gtccagttca tccagtttac gacgggagag
     6601 gacaggggag atttgttcga tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc
     6661 ctttaacttc attttatcag gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac
     6721 agtgtcatct caacttattt agagatgtga gcgatcaggt ccagaacttt gttggagtaa
     6781 ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag agcgatacca
     6841 gctttagcat cgaacacgga agtgcaaact tcgccgttga aatcggtaga tactacgtca
     6901 tcttcggtgt agcccagaac gcctttcatt tcgccttcag cagcagcttt aacggcagct
     6961 ttgatctgct cgtaagttgc agctttttcc agacgaacgg tcaggtcaac tacggatacg
     7021 ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc cattcagttc tggcagtact
     7081 ttacctacag ctttagcagc accggtagag gacgggatga tgttctggga agcgccgcgg
     7141 ccgccgcgcc agtctttgtg agacgggcca tcaacggttt tctgagtagc ggtagtagcg
     7201 tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat cgttgataac tttagccagc
     7261 ggagccaggc agttggtggt gcaggaagcg ttggaaacga tgtcctggcc agcatatttg
     7321 tcgaagttag cgcctttaac gaacatcgga gtgttgtctt tagacggacc agtcataacc
     7381 actttcttcg caccagcggt gatgtgttta cgagcagttt cgtcagtcag gaacagacca
     7441 gttgcttcag cgacaacgtc aacaccaact tcgtcccatt tcaggttagc cggatcacgt
     7501 tcagcggtaa cacggatttt tttaccgtta acgatcagat gaccgtcttt cacttcaacg
     7561 gtaccgtcga aacggccgtg agtggagtca tatttcagca tgtatgccat gtaatcagcg
     7621 tctaacaggt cgttgattgc aacgatctcg atgtcagaac gtttctgagc agcacggaaa
     7681 acaatgcgac cgatacggcc aaaaccgttg atacctactt tgatagtcat atattccacc
     7741 agctatttgt tagtgaataa aaggttgcct gtaaaattac aaaaacctta cgcagcgtca
     7801 agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt acgcgactga
     7861 ctcgcctcac tcttcctttg ggcttgagac cacatggggt cggcgccccg aattttaaag
     7921 ggcaattacg ataaaaatgt gattttcatc acgatttagg tgcagccatt tcgtcgagat
     7981 caagtttagg acaaaatttt gccagttggt gttaatgttt tgttagaatc agtcacgtaa
     8041 tgtgagcacg attaaagtga gatgtgagca aatggctaat aaaccttcgg cagaagaact
     8101 gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag aatcatggga cagagccgcc
     8161 atttacgggt cgtttactgc ataacaagcg tgacggcgta tatcactgtt tgatctgcga
     8221 tgccccgctg tttcattccc aaaccaagta tgattccggc tgtggctggc ccagtttcta
     8281 cgaaccggtg agtgaagaat ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg
     8341 catagaaatt cgttgcggta actgtgatgc ccatctgggg catgtcttcc ctgacgggcc
     8401 gcagccaacg ggcgaacgtt attgtgttaa ctctgcttct ttacgcttca ctgatggtga
     8461 aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca acttattcca caggagcgga
     8521 aaaattatga atcttgatga cattatcaac agcatgacgc ctgaggtata ccagcgtttg
     8581 tcgaccgccg ttgaactggg gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa
     8641 gagaactgcc tgcaactggt gatgctgtgg caagcccgca ataataccga agcacagcat
     8701 atgacaattg acacaaatgg tcagatggtc atgaagagca aacagcagtt aaaagaagat
     8761 tttggtatct cagcaaagcc tattgccatg tttaagtaag tggttcatcc cctttttgcc
     8821 gcggctatga aggggatagc caacaatgac cccacgactc atctcaatta tctcgccatg
     8881 acctggctaa gtcccagtcc gatctcttgc cgtcgcgttc acgcctgaat gacttctcct
     8941 gaacttaagt ctcagaccta tttggccggt aatccctctc aaatttgctc cttttgcgtt
     9001 ccgccttaca tcaacctgta acagaaccag ccattttctg cgtaattgtg atctgagttg
     9061 taaaacgcag attattatct gtgttttaca tgtttaatgt tgacctcaaa aatcatgtgg
     9121 gtaagtatga tgaccatcta agcaaaacaa aacacagagg atgacgcaat gaaagcactg
     9181 gctcggtttg gcaaagcctt tggcggctac aagatgatcg atgtgccaca acccatttgt
     9241 ggcccggaag atgtagtgat tgaaattaaa gccgcggcaa tctgcggcgc agacatgaag
     9301 cactacaatg tcgatagcgg ttctgatgag tttaactcta tccgcggcca tgagttcgca
     9361 ggttgtattg cgcaggttgg tgaaaaagtc aaagactgga aagtggggca acgcgtcgtg
     9421 tcggataaca gcggtcacgt ctgcggcgtt tgtccggcct gtgaacaagg tgattttctg
     9481 tgttgtacag aaaaagtaaa ccttggtctg gataacaata cctggggcgg tggtttttcc
     9541 aaatattgtc tggttcctgg tgaaattctc aaaattcatc gtcatgcgtt gtgggaaatc
     9601 cctgatggtg ttgattatga ggacgcagcc gtacttgacc ctatctgtaa tgcctacaaa
     9661 tccatcgcgc agcaatcgaa attcctccct ggtcaggatg tggtcgtcat cggcactggc
     9721 ccactcgggc tgttctccgt acaaatggcg cgaattatgg gggcggtaaa tatcgtcgtc
     9781 gttggtctgc aagaagatgt ggcggtccgc ttcccggttg caaaagaact gggtgcgacg
     9841 gcagtagtaa atggttctac cgaagatgtg gtggcgcgct gccagcaaat ttgtggcaaa
     9901 gacaatctgg gactggtgat tgaatgctcc ggtgccaata tcgcactgaa acaagccatc
     9961 gaaatgctcc gtccgaacgg ggaagtggta cgcgttggaa tgggcttcaa acctcttgat
    10021 ttctcgatta atgacattac cgcctggaac aaaagcatca ttgggcatat ggcctatgac
    10081 tccacctcat ggcgtaacgc tatcaggcta ttagccagcg gcgctatcaa agtcaaaccg
    10141 atgatcacgc atcgtatcgg cctgtcgcaa tggcgcgaag ggtttgatgc gatggtcgat
    10201 aaaaccgcaa tcaaagtgat catgacttac gactttgatg aataaccact ctcctgaagg
    10261 aacgttatcg catggatcag ataacaaaac cgcattgtgg tgcacggctg gatcgcttac
    10321 cggattgccg ctggcattca tcaatgtttg ccatcgtcgc gtttggcttg cttgtctgct
    10381 ggagtaatgc cgttggtggc ttgatcctcg cgcagctgaa agcgttgggc tggacagata
    10441 attccaccac agccacattc tcagcaatca cgaccgccgg aatgtttctc ggtgcgctgg
    10501 tgggcggcat cattggcgac aaaaccggtc gcagaaatgc gttcatcctc tatgaggcca
    10561 ttcatattgc ctcgatggtt gtcggtgctt tctcaccgaa tatggatttc cttattgcct
    10621 gccgttttgt gatgggagtt gggctgggag ctttactggt tacgctgttt gctggtttca
    10681 ccgaatatat gcccggtaga aatcgtggaa cgtggtcaag tcgggtttcc tttattggca
    10741 actggtcata tccgctctgt tcattgatag cgatgggact cacgccgctg attagtgcag
    10801 agtggaactg gcgggtacaa ctgcttatcc ctgcaatatt gtcgcttatc gctacggcgc
    10861 tggcctggcg ctactttcct gaatccccgc gctggctgga atcgcgcgga cggtatcagg
    10921 aagccgagaa agtcatgcgg agtatagaag aaggcgtcat acgccataca ggtaaacctt
    10981 tgccgcccgt ggttattgct gatgacggta aagcaccaca agcggtgcca tattcagcct
    11041 tactgacagg agtattactg aaacgcgtga tattaggctc ttgtgtgctg attgccatga
    11101 acgttgtgca gtacacacta attaactggt tgccaacgat attcatgacc caggggatta
    11161 atttaaaaga ctcgattgtt ttaaatacca tgagtatgtt tggcacgcca tttggcattt
    11221 ttattgccat gctggtgatg gataaaattc cgcgtaaaac aatgggtgtg gggctattaa
    11281 tcctgattgc ggtgctcgga tatatctatt cactgcaaac cagcatgttg ctcataacgt
    11341 tgattggttt cttcctgatt actttcgtct atatgtacgt ttgctatgcc tcggcagtgt
    11401 atgtccctga gatctggccg acagaggcca aactccgtgg ctccggtctg gcgaatgcgg
    11461 tagggcgaat cagtggtatt gccgcacctt atgccgttgc agtgctgctc agtagttatg
    11521 gcgttacggg agtctttatt cttctggggg cggtttcaat tattgtcgca attgctatcg
    11581 ccaccattgg aatcgaaacc aaaggtgtct ccgttgaaag tttaagtatt gatgcagtag
    11641 ccaataaata atacgggtgg attcaaaatg aaaaattcaa aagcaatatt gcaggtgccg
    11701 ggcacaatga aaattatttc agcagaaata ccagtgccta aagaagatga agttttgatt
    11761 aaagtagaat atgtcggtat ttgtggttca gatgtacatg gttttgaatc aggtccgttt
    11821 attccgccta aagacccaaa tcaagaaatt ggcctgggtc atgaatgcgc cgggacggtt
    11881 gtggctgtgg gaagccgtgt gcgcaaattt aaaccggggg atcgggtaaa tatcgaacct
    11941 ggcgttcctt gcggtcactg tcgttactgt ctggaaggca aatataacat ctgcccggac
    12001 gttgatttta tggcgacaca acccaactac cgcggcgcat taacgcacta tctgtgtcat
    12061 ccggagagct ttacttacaa actgcccgac aatatggaca cgatggaagg gacgctggtg
    12121 gagcctgccg cagtcgggat gcatgccgcg atgctggcag atgttaaacc gggtaagaag
    12181 ataattattc tgggagcagg ttgtattggt ttgatgacgt tgcaagcgtg caaatgcctg
    12241 ggagcaacgg aaattgccgt cgttgatgtg ctggaaaaac gtctggcaat ggcggaacag
    12301 cttggtgcga cagtggttat taacggcgca aaagaagaca ctattgcacg ctgtcagcaa
    12361 tttaccgaag acatgggcgc agatattgtt ttcgaaacag cgggttctgc ggtcaccgtt
    12421 aaacaggcac cttatctggt aatgcgtggc ggtaaaatta tgattgttgg tactgtaccc
    12481 ggcgcttcgg caatcaattt cctcaaaatc aatcgcgaag tcactatcca gacggtattc
    12541 cgctatgcca atcgttatcc ggtcacgatt gaagcaattt cttcagggcg attcgatgtg
    12601 aaatcgatgg tgacgcatat ttacgattat cgggatgtac aacaggcatt tgaagagtca
    12661 gttaacaaca aacgcgacat tattaaaggc gttattaaga ttagcgatta attaatacgg
    12721 gagaacagaa catgctcgca gatatcagat attgggaaaa cgatgccaca aataaacgtt
    12781 atgctattgc ccattttaat gtatggaatg cagaaatgtt gatgggcgtt atcgacgcag
    12841 ccgaagaagc gaaatcaccg gttattattt cttttggcac aggttttgtc ggcaacacct
    12901 catttgaaga tttctctcac atgatggtat ctatggcaca aaaagcaacg gtgccggtaa
    12961 taactcattg ggatcatggt cggagtatgg agattattca taacgcctgg actcatggta
    13021 tgaattcatt aatgcgtgat gcttccgcat ttgatttcga agaaaatatt cgtttaacca
    13081 aagaggctgt tgatttcttc catccgctgg gtattccggt agaggcggaa ttagggcatg
    13141 tcggtaatga aaccgtttat gaggaagcgc tggcgggcta tcactatacc gatcctgacc
    13201 aggcggctga atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc
    13261 agcatggggt ttatacgtca gagccacaat tgaactttga ggtcgtcaaa cgcgtacgcg
    13321 atgcagtgtc tgttccgctg gttttgcatg gcgcatcggg gatcagtgat gccgacatta
    13381 aaactgcaat ttcgttgggt atcgcgaaaa tcaacatcca tacggagctc tgtcaggccg
    13441 caatggtcgc agtgaaagaa aaccaggatc agcctttcct gcatttagaa cgtgaagtac
    13501 gtaaagctgt aaaagaacgg gcattggaaa aaattaaact gttcggttct gatggcaaag
    13561 cggaataatg atatggataa tctcgacgtt atttgtatag gtgccgctat tgttgatatt
    13621 ccattgcaac cggtcagtaa aaatatcttt gatgtggatt cttatcctct tgaaagaatc
    13681 gcaatgacca ccgggggtga tgcaataaat gaagcaacaa ttatttctcg tctgggccat
    13741 cgtacagcat taatgagccg tattggtaaa gatgccgcag ggcagtttat tctcgaccat
    13801 tgccgcaaag agaatattga tattcaaagc ctgaagcagg acgtcaacat agatacctct
    13861 attaatgttg gactggtgac ggaggatggc gagcggacgt ttgtcacaaa ccgtaacggc
    13921 agtctgtgga aattgaatat tgacgatgtt gattttgcgc ggttttccca ggcgaaatta
    13981 ttatcactgg ccagtatttt caacagtcca ctattggatg gtaaagcgct aacagaaatt
    14041 tttacgcaag ctaaagcccg gcagatgaat atctgtgccg atatgattaa accgcggttg
    14101 aatgaaacgc tggatgatat ttgcgaagca ttgagttatg tcaattatgc cgaggcaaaa
    14161 ttactcaccg ggaaagagac actggatgaa attgctgaca gctttcttgc gtgcggcgta
    14221 aaaacggtgg tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg
    14281 atgaaggtgc cggcggtcgc aggaataacc gccatcgaca ccattggcgc gggcgataac
    14341 tttgcttcag gttttattgc ggcactgtta gaaggaaaaa atctgcgtga atgcgcacgc
    14401 tttgccaatg caacggcggc tatctcggtt ctaagcgtcg gtgccaccac cggcgtaaaa
    14461 aacagaaagc tggtcgaaca attgctggaa gaatacgaag gataatgaag gcaaatgaaa
    14521 aagatacctt taggcacaac ggatattacg ctttcgcgaa tggggttggg gacatgggcc
    14581 attggcggcg gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat tgatacgatt
    14641 cttgaagccc atcgttgcgg cattaacctg attgatactg caccaggata taactttggc
    14701 aatagtgaag ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta
    14761 gaaaccaaat gcggcattgt ctgggaacga aaaggaagtt tattcaacaa agttggcgat
    14821 cggcagttgt ataaaaacct ttccccggaa tctatccgcg aagaggtaga agccagcttg
    14881 caacgtctgg gttttgatta catcgatatc tacatgacgc actggcagtc ggtgccgcca
    14941 ttttatacgc cgatcgctga aactgtcgca gtgcttaatg agttaaaggc tgaagggaaa
    15001 attcgcgcta taggcgctgc taacgtcgat gctgaccata tccgcgagta tctgcaatat
    15061 ggtgaactgg atattattca ggcgaaatac agtatcctcg accgggcaat ggaaaacgaa
    15121 ctgctgccgc tatgtcgtga taatggcatt gtggttcagg tttattcccc gctagagcag
    15181 ggattgttga ccggcaccat cactcgtgat tacgttccgg gcggcgctcg ggcaaataaa
    15241 gtctggttcc agcgtgaaaa catgctgaaa gtgattgata tgcttgaaca gtggcagcca
    15301 ctttgtgctc gttatcagtg cacaattccc actctggcac tggcgtggat attaaaacag
    15361 agtgatttaa tctccattct tagtggggct actgcaccgg aacaggtacg cgaaaatgtc
    15421 gcggcactga atatcaactt atcggatgca gacgcaacat tgatgaggga aatggcagag
    15481 gtcttggggc gttaaatact caacacgatt cacctctgtt tctgtgaaaa ctgtgatgaa
    15541 aatctgtgat tcaaacaggt tattttgaaa gtaaacatcg gttatgcgat aatcgcgcta
    15601 atgtgatgta aaaagataca ggggtgtaat cgtggcggca aaagacagga ttcaggcaat
    15661 taagcaaatg gttgccaacg ataaaaaggt gacagtctca aatttgagtg ggatttttca
    15721 ggtaaccgaa gaaaccattc gccgcgatct tgagaagctg gaagatgaag gctttttgac
    15781 cagaacctat ggtggtgctg ttttaaatac agcgatgttg acggagaata tccattttta
    15841 taagcgcgct tcatcgtttt atgaagagaa gcagcttatt gcacgcaagg cactaccctt
    15901 tatcgacaat aaaaccacca tggcagccga ttccagtagt accgtaatgg aattgctcaa
    15961 attgttacag gaccgtagtg acctgacgtt gctaaccaac tcggcggaag cgattcatgt
    16021 tctggctcag tcagaaatta aagtcgtttc aacgggtggg gaactaaaca aaaatacact
    16081 ttcactgcaa ggaagaatta ctaaagagat catcagccgc tatcatgtcg atatcatggt
    16141 aatgagttgt aaaggtcttg atattaacag cggcgcgctg gactctaatg aagcggaagc
    16201 tgaaatcaaa aagacaatga tccgtcaggc gacagaagtt gcgttattgg ttgatcactc
    16261 taagtttgat cgcaaagctt ttgtccagtt agccgacttt agtcatatta attacataat
    16321 aactgataaa tcaccgggtg cagagtggat agcattttgc aaagacaata atatccagct
    16381 ggtgtggtaa tttaagcacg cgttgtcaat attatttgtt cctgcgcctg gaaataaaat
    16441 gatgataaag atgcggcaag tgaatttgcc gcaattgcta ccttttttag caaatccgag
    16501 gcacccaatg gaacaatatg atcaaattgg cgcaagactg gaccgcttgc ctttggcccg
    16561 gtttcattat cgtatatttg gtattataag ctttagtctg ttatt
//
LOCUS       DAZGXS010000101        16573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGXS010000101 DAZGXS010000000
VERSION     DAZGXS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(284..709)
                     /gene="arsC"
                     /locus_tag="ACHKG5_003424"
     CDS             complement(284..709)
                     /gene="arsC"
                     /locus_tag="ACHKG5_003424"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0869460.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(722..2011)
                     /gene="arsB"
                     /locus_tag="ACHKG5_003425"
     CDS             complement(722..2011)
                     /gene="arsB"
                     /locus_tag="ACHKG5_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0869461.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2065..2418)
                     /gene="arsR"
                     /locus_tag="ACHKG5_003426"
     CDS             complement(2065..2418)
                     /gene="arsR"
                     /locus_tag="ACHKG5_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0869462.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3162..3245
                     /gene="dinQ"
                     /locus_tag="ACHKG5_003427"
     CDS             3162..3245
                     /gene="dinQ"
                     /locus_tag="ACHKG5_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0869463.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3299..4651)
                     /gene="gorA"
                     /locus_tag="ACHKG5_003428"
     CDS             complement(3299..4651)
                     /gene="gorA"
                     /locus_tag="ACHKG5_003428"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0869464.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4723..5565)
                     /gene="rlmJ"
                     /locus_tag="ACHKG5_003429"
     CDS             complement(4723..5565)
                     /gene="rlmJ"
                     /locus_tag="ACHKG5_003429"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0869465.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5768..7810
                     /gene="prlC"
                     /locus_tag="ACHKG5_003430"
     CDS             5768..7810
                     /gene="prlC"
                     /locus_tag="ACHKG5_003430"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0869466.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7818..8570
                     /gene="rsmJ"
                     /locus_tag="ACHKG5_003431"
     CDS             7818..8570
                     /gene="rsmJ"
                     /locus_tag="ACHKG5_003431"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0869467.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8619..10088)
                     /gene="dtpB"
                     /locus_tag="ACHKG5_003432"
     CDS             complement(8619..10088)
                     /gene="dtpB"
                     /locus_tag="ACHKG5_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0869468.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10254..10307
                     /locus_tag="ACHKG5_003433"
     CDS             10254..10307
                     /locus_tag="ACHKG5_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869469.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10285..10338
                     /locus_tag="ACHKG5_003434"
     CDS             10285..10338
                     /locus_tag="ACHKG5_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869470.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10405..10839)
                     /gene="uspA"
                     /locus_tag="ACHKG5_003435"
     CDS             complement(10405..10839)
                     /gene="uspA"
                     /locus_tag="ACHKG5_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0869471.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11230..11565
                     /gene="uspB"
                     /locus_tag="ACHKG5_003436"
     CDS             11230..11565
                     /gene="uspB"
                     /locus_tag="ACHKG5_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0869472.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11636..13135)
                     /gene="pitA"
                     /locus_tag="ACHKG5_003437"
     CDS             complement(11636..13135)
                     /gene="pitA"
                     /locus_tag="ACHKG5_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0869473.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13367..14569
                     /gene="yhiN"
                     /locus_tag="ACHKG5_003438"
     CDS             13367..14569
                     /gene="yhiN"
                     /locus_tag="ACHKG5_003438"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0869474.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14887..15939)
                     /gene="yhiM"
                     /locus_tag="ACHKG5_003439"
     CDS             complement(14887..15939)
                     /gene="yhiM"
                     /locus_tag="ACHKG5_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP0869475.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            16323..>16573
                     /locus_tag="ACHKG5_003440"
     CDS             16323..>16573
                     /locus_tag="ACHKG5_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869476.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWF"
BASE COUNT         4311 a         4154 c         4263 g         3845 t
ORIGIN      
        1 actgataaat cataatccag tacgttttta aactgctgca ctgtatcgct gatcactaag
       61 cagctcactg atacaccatg ctggacgcga taattggaat ctcacgcagg gagtaatgtg
      121 taaatagcaa aatcgcctga tgcgctacgc ctgtcaggcc tacaaataac atacaattta
      181 ttgaattttc atgactttgt agatcggata agacgcgtta gcgtcgcatc cgacataaat
      241 aaagcgcact tttctaacaa cctgtggggg ggatatagcc gctttatttc aggcgcttac
      301 ccgcttcatc aaccactttc tcgccatctt ccttggtgaa tgcgcctttt tgcgcatctg
      361 gcagaatttc cagcaccact tctgaagggc ggcacaggcg agttcccagc gtcgtcacca
      421 caatcgggcg attaatcaga atcgggtgct gaagcataaa gtcgattaac cgatcgtcag
      481 taaatttatc ttccgcaagg cccagctcct catacggttc gacgttttta cgcagcagcg
      541 cgcgtacgga aatccccata tcggcaatga gtttgaccag ttcatcgcgc gttggcggag
      601 tttccagata atggataatg gtcggttctg tgccgctgtt gcggatcatc tccagcgtat
      661 tacgcgacgt gccgcaggcc gggttgtgat aaatggtaat gttgctcata tcagtatctc
      721 attacaaagt gaaagagaga cgtagcgcca gcgcagccag cgtcacaaac agcacaggca
      781 gggtcatgat aatccctgta cggaagtaat atccccagct gatagtcata ttcttctgcg
      841 aaagtacgtg cagccagagc agcgttgcca ggctaccaat cggggtgatt ttcggcccca
      901 aatcgcagcc aatcacgttg gcataaacca tcgcttcttt gataacgcca gatgccgtgc
      961 tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat attgttcata atagaagaga
     1021 ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca caggccgtta tccgccagca
     1081 cgttgagtac gccagaaaga tattccgtta atccggcatt gcgcaggcca taaaccacca
     1141 gatacatgcc gagcgagaag atgacaatct gccagggcgc accgcgcagc actttacccg
     1201 tattaatcgc atgaccgcgt ttagcgacga caaataatat caacgcgccc actgccgcaa
     1261 tggcgctgac cggaatgccg agcggttcca ggacgaaaaa tcccaccagc agaagcagta
     1321 aaacaaccca gccagttttg aacgtagcag gatctttaat cgcttctgcg ggagatttca
     1381 gcagcgccat gtcgtagttc tgcggaatat ctttgcggaa atagagatgc aacatcacca
     1441 gcgtggcaac aatcgcggcg atatccaccg gcaccatcac cgaggcatat tcgcgaaagc
     1501 cgaggccaaa gaaatctgcg gaaacgatat tcaccaggtt ggagacaata agcggcaggc
     1561 tggcggtatc ggcaatgaat ccggccgcca tcacaaacgc cagcgtagtg cctttactga
     1621 accctaaagc cagcagcatg gcgatgacaa tcggtgtcaa aataagcgcc gcgccatcat
     1681 tggcaaacag ggcggcaacg gcagcaccga gcaggacaat ccaggtaaac agcaagcgac
     1741 cgcgtccatt gccccagcgt gaaacgtgca gtgccgccca ttcaaaaaag ccggactcat
     1801 ccagcagcag gctgatgata ataacggcaa taaacgcagc cgtcgcgttc cagacgatat
     1861 tccacaccac cggaatatcg cccggatgta ctacgcccgt aactaacgcc agtactgcgc
     1921 cgagcgttgc actccagccg atgcctaaac ctttcggctg ccagataacc aatacaatgg
     1981 tcaggacaaa gatagcgcct gccagtaaca taatgcctcc cggaaaaaca catgtgcaaa
     2041 tccatatgtg tttagataaa ttttttaact acaagtgttc ttactgtcta cggaacaatt
     2101 ttgtctggcc aggttgcgga caatcacctg aaccttttcc tgttcgcatc gccaggcctg
     2161 ctcaataatt ttcgcggccc atgatggaat atgcggtgat aagcggtaat gaacccactt
     2221 accctgcttg cgatccagca acaaaccgct ttcacgaaga agcgccagat ggcgggagat
     2281 cttgggctgc gactggtcga gagcagtgca gagatcacag acgcataact ctcccagctc
     2341 gctgagcagt aaaacgatgc ccagacgggt ttcatcggca agaattttga acagttggat
     2401 gggtaacaga aatgacatat tgcgctcctg attgttgcag gtagtgtctc tcttcgaagc
     2461 ggataagtca aaaacatata tggtttaacg aatgtgtatg tgcagaggaa ggtaataggt
     2521 gtgaattaag cgttggctat tcatttgaaa ggaggtctga atcaggacgt aaaaaaaccg
     2581 actttgcgtc ggttttttac tttccagccc agagttggtg gctctggctg gagtgagaat
     2641 gcccacatct aacggttcaa tactattagc cagtggctaa cattgcaagc gttagcgatt
     2701 tcttcagctg gagaagggac agtttgctat acatgttggt gaaagaggat ttatatactt
     2761 tcatcgggtc gctgccgtac caggctttgt ccgaatccgg atgtaaaaaa accgacttcg
     2821 cgtcggtttt tttactcttc agccctgagt tggtggctct ggctggagtg agaatgccat
     2881 catctaacgc atcaatacta ataccatact gaaactattg caaggacatg ctggttttat
     2941 aacctgaata tactgtatga ttatccagtt agctctgagg cattttcact ctggcaatgc
     3001 gcataaacgc tttcaaagtc ctgttcagtg gtacgggtgg tgccgttaac tgatgctctg
     3061 gccggagtga gaataccatt atctaacaat agggcatgcg ccgtgacagg cgatggatga
     3121 gtaagcggat gcattctcac tccatcgtat ggagaacacg ggtgattgat aaagcaatca
     3181 tcgttcttgg ggcgttaatt gcgctgctgg aactgatccg ctttctgctt cagcttctga
     3241 actgatagcg gaaacgtaat taagggcaaa gagcacacca ctctttgccc tttaacattt
     3301 aacgcatggt cacgaactct tctgccgccg ttgggtgaat ggcgacggta ttatcgaagt
     3361 cttttttggt tgcgcccatc ttcagtgcca ccgcgaagcc ctgcaacatt tcgtccatac
     3421 caaaaccaat gccgtgaata ccgacaatct tctcttctgg cccaacgcac accagcttca
     3481 tgcggcacgg ctggcggtga gtggtgacgg cggtatacat cgcggtgaaa gaggatttat
     3541 acactttcac ctgatcgtcg ccgtactgct cgcgcgcctg cggttccgtt aaaccaacag
     3601 taccaatcgg cggatggctg aagaccacgg tcggaatgtt gctgtaatcc agatgctcat
     3661 ccggcttgtt attaaacagg cgttcagaga gacggcgacc cgctgcaact gccaccggtg
     3721 tcagttctac tgcacccgtg ttatcgccca ccgcgtaaat accttcaacg ttggtgtttt
     3781 gatatttatc gacgacgata tagccttttt cgttggtttt aacgccagcg gcttcgaggt
     3841 tgatgttgtc attggcaggc tcgcggccaa tcgcccaaat caggcaatcc accgtttcac
     3901 tgcgaccatc ttccagctcc agcgtcaggc taccatcggc atttttcact accgctttcg
     3961 ggatggcgtt ggtgtgcaac tgcgggcctt cggcgttcat cacttcgacc agcgtttcag
     4021 agatcatcgg gtcgaagctg cgcagcggcg catgtttacg cacaaacaga tgcgttttcg
     4081 cgccgaggcc gttaatcacg ccagccagct caacggcgat gtaacccgcg ccaacaaccg
     4141 ccacacgctc tggcaaagca ggaagggcaa agaaaccatc agaatcaata ccgtattcca
     4201 cgcccggaat atccgggtgg ctcggacgac cgccggtggc gatcagaata tgatcggctg
     4261 tgatggtttc accgttaact tccagcgttt tggcatcaac gaagcgggca aagcctttga
     4321 ttacatcaac gttattttta ccgagcacgt tttcatagga agtatgaata cggtcgatat
     4381 aggcggtacg gctggcgatc aacgtttccc agttgaattt attgatagtg gtatcaaaac
     4441 cataatccgg gccgtacata tggatcgctt cacggatttg cgccgcgtgc cacatcactt
     4501 tcttcggtac gcagccgacg ttcacgcagg tgccgcccag ctctttggct tcaatcagcg
     4561 cgcatttctg accgtacata gccgcgcggt tgatggaggc gataccgccg ctgccgccgc
     4621 cgatggcgat gtaatcatag tgtttagtca tgacagagtg tccttatcgt tgattaccgc
     4681 gattgtagcg cgagacgtaa taggtgccag caatggctgc aattactctg gcacgatcca
     4741 gcttacggtg gcgtgcccgg tgccagctgg aaccagtttg ctgtgcagcc acggcagcac
     4801 gttattcatc tgctgttcca gtttccacgg cgggttaatc acaatcatgc cggaagcggt
     4861 catgccacgg cgatcgctgt ctggcagtac cgccagttca atttgcagaa ttttgcgaat
     4921 gccggtcgct tccaggtcgt ggatcatgcg cttaatttgc tggcgcagca ccaccggata
     4981 ccacaatgcg taagtgccag tggcgaaacg tttgtaacct tctgctatcc cgctgactac
     5041 cgcctgatag tcggttttca tttcatacgg cggatcgata aggattaaac cacggcgaga
     5101 aaccggcggc agtttggcct taagctgctg gaaaccgtcg gatttttcga cgcgcgcacg
     5161 gctgtctttc tgaaattcag aacgcagcaa cgggtaatcg ctcgggtgca gttcggtcag
     5221 ttgcaggctg tcctgttcac gcagtaactg acgggcaatc aacggtgaac cggggtagta
     5281 acgcaactga ccgctacggt tgaagtgttt taccacattg atgtacggct ccagctccgc
     5341 aggcaaatcg tcctgctgcc agatacgggc gataccttcg agatattcgc cggtacgctc
     5401 ggcatgttcg ctgcctaact gataacgccc ggcccctgcg tgggtgtcga gatagagaaa
     5461 cggtttatct ttctctttca gcgactcgat gatcaggctc tgaacggtat gtttaaggac
     5521 gtcggcgtgg ttgccagcgt gaaagctgtg gcgataactg agcatgggta aaggtgttcc
     5581 ggtaagtaaa aatcggcccg tattcggggc gcacaaaagc ctatcaggac agtataccga
     5641 aaagaggccg ccgccgcgaa agcgtaacgt ttctcattga aattcactac acttaacccc
     5701 atgctacaaa cattatgtaa agcgcctgtt gagcgcttcc ttaacctctt taaccaggac
     5761 tgcgctaatg acgaatccgt tactgactcc ctttgaattg cctccgtttt ctaaaattct
     5821 cccggaacat gtcgttccag ccgtgactaa ggcattgaat gactgccgcg aaaatgtgga
     5881 gcgcgtagta gcgcaaggag caccgtacac ctgggaaaat ctctgccagc cgttggcgga
     5941 agtggacgat gtgttggggc gtattttctc cccggtcagc cacctgaact cggtgaaaaa
     6001 tagcccggaa ttgcgcgaag cgtacgaaca aactctgccg ctgctgtcgg aatacagcac
     6061 ctgggtaggg caacatgaag ggctgtataa agcgtaccgc gacctgcgcg atggcgatca
     6121 ttacgccacg ctgaacacgg ctcagaaaaa agcggttgat aacgcactgc gcgattttga
     6181 actctcaggc atcggtctgc cgaaagagaa acaacagcgt tacggcgaaa ttgccactcg
     6241 tctttctgaa ctgggcaacc agtacagcaa caacgtcctt gatgcgacga tgggctggac
     6301 taaactcgtt accgacgaag cggaactggc ggggatgcca gaaagcgcgc tggctgcggc
     6361 aaaagcccag gccgaagcga aagagctgga aggctacctg ttgacgctgg atatcccaag
     6421 ctacttgccg gtaatgacct actgcgacaa ccaggtcttg cgtgaagaga tgtatcgtgc
     6481 ttacagcacc cgcgcttcag atcaaggtcc aaacgccggt aaatgggata acagtaaggt
     6541 gatggaagag atcctcgcgc tgcgtcacga actggcgcaa ctgctgggct ttgaaaacta
     6601 cgcgtttaaa tcactggcta ccaaaatggc ggaaaacccg cagcaggtgc ttgatttctt
     6661 aaccgatctg gcaaaacgcg cgcgtccgca gggcgaaaaa gagctggcgc aactgcgtgc
     6721 cttcgccaaa gccgaatttg gcgtggatga gttgcaaccg tgggatatcg catactacag
     6781 cgaaaaacaa aaacagcacc tctacagcat cagcgacgaa caactgcgtc cgtacttccc
     6841 ggaaaacaaa gcggttaacg gcttgtttga agtggtaaaa cgtatttacg gcatcaccgc
     6901 taaagagcgt aaagatgttg atgtctggca cccggatgta cgtttcttcg aactgtatga
     6961 cgagaacaac gaactgcgcg gcagtttcta cctcgacctg tatgcccgtg aaaacaagcg
     7021 cggcggggcg tggatggatg actgcgtagg ccagatgcgt aaagccgatg gttcgctgca
     7081 aaaaccggtc gcgtatctga cctgtaactt caaccgcccg gtgaatggta aaccggcgct
     7141 gttcactcac gacgaagtga tcactctgtt ccacgagttc ggtcacggcc tgcaccatat
     7201 gctgacccgc atcgaaaccg ccggtgtttc cggtatcagc ggtgtgccgt gggatgcggt
     7261 cgaactgccg agtcagttta tggaaaactg gtgctgggag ccggaggcgc tggcgtttat
     7321 ctctggtcac tacgaaaccg gcgaaccgct gccgaaagag ttgctggata aaatgctggc
     7381 ggcgaagaac taccaggcgg cgctgtttat tctgcgccag ctggagttcg gcctgttcga
     7441 tttccgcctc catgccgagt tccgcccgga tcagggagcg aaaatcctcg aaactctggc
     7501 agaaatcaag aaactggttg ccgtagtacc gtctccatcc tggggccgtt tcccgcacgc
     7561 tttcagccat attttcgccg gtggttatgc cgcaggttac tacagctacc tgtgggctga
     7621 cgtactggcg gcagatgctt tctcgcgctt tgaggaagag ggcattttca accgtgaaac
     7681 cgggcagtcg ttcctcgaca acattctgag ccgtggcggt tcagaagagc cgatggatct
     7741 gttcaaacgc ttccgtggtc gtgaaccgca gctggatgcg atgctggagc attacggcat
     7801 taagggctga tcattcagtg aaaatctgct taattgatga aacaggcacc ggagacggtg
     7861 ccttatctgt tctggcggcc cgctgggggc tggagcacga tgaagacaac ctgatggcgc
     7921 tggtgttaac gccggaacat ctggaattgc gcaagcgtga tgagccaaaa cttggcggca
     7981 tctttgttga ttttgttggc ggagcgatgg cgcaccgacg caaattcggc ggtggtcgcg
     8041 gtgaggcggt ggcgaaagcg gtgggcatta aaggcgatta tttgccggat gtggtggacg
     8101 ctaccgcagg gctggggcgc gatgcctttg tgctggcttc ggtcggctgc cgcgtgcgga
     8161 tgctggagcg taatccagtc gttgccgcgc tactcgacga cggcctggcg cgtggatatg
     8221 cggatgcgga aatcggcggc tggttgcagg agcgtttgca gttaattcac gcctccagcc
     8281 tgacggcgct gaccgatatt accccgcgtc cgcaggtggt ctatctcgac ccgatgttcc
     8341 cgcataagca gaaaagcgcg ctggtgaaaa aagagatgcg cgtctttcag tcgctggtgg
     8401 gaccggatct tgatgccgat ggattactgg agcctgcacg cttgctggcg accaaacgcg
     8461 tggtggtaaa gcgcccggac tacgcgccac cgctagcgaa tgtcgccacg ccaaacgcgg
     8521 ttgtgactaa agggcatcgc tttgatatct atgcaggtac gccagtgtaa aagtaaagca
     8581 gggtagcgat aacgctaccc tgctttctgc aagaataatt aatggctttc cggcgtcgca
     8641 atcatgcgtt tcagccacgg caccatcaac agcatcacta ctgcaacgcc cagcgtgacc
     8701 agaccaatct taccaaacac gttggtatag acaggcaacg tctcaagcgg atcggtaatg
     8761 ttgtctggta ctgcggtaaa tgttgccaca tagccgccca gcaagaacgc ggcagcctgt
     8821 gtcaggaacc acatcccgag aataaagccc atcaaatgct gcggcaccag agcagcaatc
     8881 atcgccaggc caagggcgct aataaacagt tcacctaagc tctggaataa gtacaccagc
     8941 acgataaacc atggcgatgt cagcccttgt gcatccgcaa accacattcc cgcagctgcc
     9001 gccgtcaaaa agcccagtga gcacataaac atgccgagag taaatttcat cggcatcgag
     9061 aggtctttgc ctttgttacc cagatgcgtg taaatgcctg ccagtattgg gctggcgagt
     9121 accacccaga acgggttaag cgcctggaag ctgaccgggt tgatggaaaa accgagaatt
     9181 tcatgatgca cgttgttgat ggcaaagaag ttcagcgatg ttggcatctg ggcgtagaga
     9241 atgtaaaaca ccaccgcttc gagcatcagg acaaaggcga caaacatttt attgcgcccg
     9301 gttttatcca gcttgaatgc ctgacgaaag aagatgatgg tgacgacgat ggagagaaca
     9361 atcagcacca gattggcgac ttctacgttg tgcatcagcc aggcgcagac gaagatcatc
     9421 accacgctgc caagtaatac gtacaacagt ttgctgaagc tcattggccg gaagtcgggc
     9481 tcagaaccaa tgtctttcac cattccacga caggcgatgt aaaccagtaa tgcgataatt
     9541 aaccccgcac cgcacaggtt gtaggtgact gaatagccga atttatcagc gatcacaggg
     9601 gccagcgata acgctatcaa cgagccgata ttgatcgaca tatagaacag ggtgaatgtg
     9661 ccatcaaggc gcggatcttt cggcggatag cacttcgaaa gcaagctggc tgggttagct
     9721 ttaaacaggc cgttaccgac agcgatagtc cccagggcga tgaaaatcag gtcaggctta
     9781 agtagcgaca tgccggtcat gaagtagcca atcgccagca caagtgctcc aagaacgatg
     9841 gtgcgtttgg tccccagcag gtggtcgccg acatagccgc caatggaaat gaggccatag
     9901 accagtgcag caaaagcacc aaaagtgaca aaagcctgct cttgcgagaa tccaagctgt
     9961 ttaacgaaga aaaccgccag tacgccctgc acgccgtagt agccgaatcg ctcccataac
    10021 tcgacaaaaa agatcatgaa aaatgggcga ggttgctgca gcatccccat gggtgttgtt
    10081 gtattcatat ttattaacct tccaatacca tcctgaaaag tatgaacgcg taagggataa
    10141 cgcgcgaaaa agtttacatc tgtcagagat tcttaatatt gatgcggtga atagattaat
    10201 acactattac aacagaaaat aaccagctga tcacactaat gatggaaaat atgatggtgt
    10261 taaatattgt attggctggg gattttgatt gcgaacgcag accgtaggcc agataaggcg
    10321 tttacgctga tcaggtaaca aggcgcgaca aaaagcaaaa cgcccgcata tgcgggcgtt
    10381 caggaacacg tcgtggaggg aagattattc ttcttcgtcg cgcagcggaa caatcagcat
    10441 atcaacgtga acggtgttga tcagctgacg tgcggaagac atcagtttgc tccagaagtc
    10501 ctggtggtga ccacaaacca ctagatccat atcgtatttc ttgattgcat cgaccagaac
    10561 ctggcccagg tcgccgctgc cgctcagggt ttcagtgatt gggtagcctg cattagtgga
    10621 aagctcggtc agcgcatgat gtgtctcttc agagatgcgt ttctgcatat caccaagatt
    10681 cacatcaata agcccggtat ataggtcaga gtagtttaca tctacgtgga tcagagaaac
    10741 tttcgcattg tatgggcgag ccatagagac tgctttctct accagaactt tgctttccgg
    10801 ggagagatcg accgcgatga gaatgtgttt ataagccata gtgttactcc ttccataaag
    10861 ttgtcgatga ctggccagct agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac
    10921 tacgtgcgcg gggctaaccc agccgtaaag attcagtgtt aagaccatcc ttaccttata
    10981 gcgacccgga tgatccgtca atccgccttg cttacccgtt agataaacaa attttcgaca
    11041 tgaatatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
    11101 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
    11161 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
    11221 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
    11281 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
    11341 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
    11401 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
    11461 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgaccag cgcattgtgt
    11521 ggtctggtgg tggtcagcct gattgccttg atgatttggc attaagcact tcagcaataa
    11581 taacgcataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tactattaca
    11641 ggaactgcaa ggagagccag taaagcccgc cggaaagcag taccgcagcc ggaagggtaa
    11701 acacccaggc catcagaatg ctggtcacgg ttttacgctg taagccgcca ccatctacca
    11761 ccatcgtccc cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc atcccggtat
    11821 aactcgccag gccgatagac actgccgcag tcatctgggc agacatcccc tgagcgtagg
    11881 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
    11941 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggt gcatactcga
    12001 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc
    12061 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
    12121 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
    12181 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
    12241 ttgcgccagc ttccggagcc ggtactaact gatcagcacc agtagcttgt ttcagcagcg
    12301 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
    12361 cgtagccagt ggcattcatg ttcaccacga agcctgctgg tgcgacacca atcaatacca
    12421 acataaccag accaatgcct ttctgaccat cgtttgcgcc gtgcgaaaac gccacgccga
    12481 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    12541 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    12601 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    12661 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    12721 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    12781 atgcaggcaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    12841 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    12901 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    12961 ccgctgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    13021 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    13081 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    13141 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    13201 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    13261 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    13321 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    13381 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggc
    13441 aggacgccgg gttctgctga tcgataatgg taaaaaacca gggcgcaaaa tccttatgtc
    13501 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccgggcgctt atctgagcca
    13561 gaatccgcat ttttgtaagt ctgcactcgc gcgttttacc cagtgggatt tcattgatct
    13621 ggtcaataaa cacggcatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    13681 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtgaccgt
    13741 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgat acaggcttca cgcttgaact
    13801 gaacggcatg actgtcggtt gtgaaaagct ggtcatcgcg accggcgggc tgtcaatgcc
    13861 gggcctgggc gcgtcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    13921 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    13981 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    14041 tgagaactta ctcttcaccc atcgcggctt gtctggaccg gcggtgttgc aaatttccag
    14101 ctactggcaa ccgggggaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    14161 cttcctgaat gagcagcgta acgcacatcc gaatcagagc ctgaaaaaca cactggcggt
    14221 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    14281 gctaaaacag ctcaacgtgc gtgaccaaca ggcactgatt agcacattga ccgactggcg
    14341 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    14401 caccaacgaa ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtactttat
    14461 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    14521 gagtgcgtgg gcttgtgcgc aggatttgat tgcagcgaag tcgtgctgag ctatttttcc
    14581 taaagatgaa aattattaga gtcgcattaa aaatgagcta attttgatag tggctatctt
    14641 gtgattattt tctaatgagc ccgtgaactg aaccccccat gcttaatata aggtggaggg
    14701 aaaggtgatt gaaaactcac ccagtgggct gatctttata aaatatgaac aggttgaaaa
    14761 aaaaacagta actttgctgt tttttatact taaccactat gcattaatgc tgcgttattt
    14821 cattctacct aagaaaaacc agcgttacgc aaatggttaa cgctggtttt atccggtacg
    14881 ttgcaattat tttttagcag aacccgcttc taatattgaa acgattgaga acaacgtaaa
    14941 gcataccgct cccaaaccga ccagaacccg cgaaggaata aaatatccca tatcggtctg
    15001 cgccatttcc gcaagaaacg atgctaggaa aaggcaaaac aggcaggtga agacgggaat
    15061 catcggtatt ctgttggcta acgaacaggt acgtctccag accagtgcca gtagccagac
    15121 ttttgagaat atgctgtaac agatcattcc caagcaaatg aggataatac cgggagccag
    15181 tcgggcgctt gcatcagaac tgactaagac gtatatgccc tgcagcaccg tgattgagcc
    15241 gagaaaaata acccaataac accacagcca gtgctctttc gttgaaaacg tattacgtgt
    15301 ttgatggacg attgtggcaa caaggccaat tagacaggca cagattgcgg ttaacccgag
    15361 caatacgtga ccggcgacat aatgcggagt tatgtcggca ctacgtaaca gcgtaataga
    15421 ccaaatgaaa ccgagcaggg tgagtaacac gggaacggca atgaggcaat taccgattaa
    15481 tgaagaataa gcctgtaccg gtgttccgtc gctcttgctc cccgctgcgt ttttaggaat
    15541 gagcagaaag tgaccggatg acgctgccac cgtcgataca caggcggcaa tcataccaac
    15601 gccgaaaata acatggccgg cgacaaactc gtctgccatc acatcattac ctgctaacag
    15661 tgcccaaccc catatcatgg tgataattga ccccgcatag ccaataatgg ggaacaatgt
    15721 attgtaaaac gtattaacgc cgcgcgtgag ctgcgaaatg ataataaatg cggtagtgaa
    15781 taatgccagg catatggctg ccagagaaat gagcacatga cccgcgacga aatactcgct
    15841 ttgccctgaa ctttccagca caaagccacc taacgcgata caaatcaagc ccataattaa
    15901 agggattaat ttgaaaagcc acccgatata tatgttcact ttaaactcct caggtatccc
    15961 tgctaatatt gaaatgtgca ttaatgataa tttcagcctg ttaatacaat gacgagatat
    16021 cattaaagac aaatgcatag cataactata gcggggttta ctttgcagag cgctactttt
    16081 cagcgttcaa aaggccatga taaataaata tttatcctaa gattaattct tattctgttg
    16141 ttttttacaa ttatcttcca gggagggatt ctttttcatt atttggtaaa agaccggatg
    16201 gtctcggtgc ttaaaatcag cgagtatata cgcatcattg ctttatatcg tcaaaaaata
    16261 tgtctttcag acatgtatca tttattttgt tactaacaag tcaaaaatat cacgggttat
    16321 ttatgaaagt atttcacaat atatttaagt acatctcttc aaaccatcaa gataagcatt
    16381 cagataaagt taataatcat caacaccatg gcaaggttga taaaaaacat cgttcagaga
    16441 tagttgaaat tgataagctg gataaacatt cgcaaataga taacgatttt ggcctgcata
    16501 tcatctattt cctgcaacat ggctgctgga acgtaactga acatagtcct cagatagaga
    16561 aaatatggtt tta
//
LOCUS       DAZGXS010000102        16229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGXS010000102 DAZGXS010000000
VERSION     DAZGXS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            264..1163
                     /gene="yegS"
                     /locus_tag="ACHKG5_003441"
     CDS             264..1163
                     /gene="yegS"
                     /locus_tag="ACHKG5_003441"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0869477.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1245..2018)
                     /locus_tag="ACHKG5_003442"
     CDS             complement(1245..2018)
                     /locus_tag="ACHKG5_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0869478.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(2124..3164)
                     /gene="gatD"
                     /locus_tag="ACHKG5_003443"
     CDS             complement(2124..3164)
                     /gene="gatD"
                     /locus_tag="ACHKG5_003443"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0869479.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3212..4567)
                     /locus_tag="ACHKG5_003444"
     CDS             complement(3212..4567)
                     /locus_tag="ACHKG5_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0869480.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4571..4855)
                     /gene="gatB"
                     /locus_tag="ACHKG5_003445"
     CDS             complement(4571..4855)
                     /gene="gatB"
                     /locus_tag="ACHKG5_003445"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0869481.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4886..5338)
                     /gene="gatA"
                     /locus_tag="ACHKG5_003446"
     CDS             complement(4886..5338)
                     /gene="gatA"
                     /locus_tag="ACHKG5_003446"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0869482.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5348..6610)
                     /gene="gatZ"
                     /locus_tag="ACHKG5_003447"
     CDS             complement(5348..6610)
                     /gene="gatZ"
                     /locus_tag="ACHKG5_003447"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0869483.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6639..7493)
                     /gene="gatY"
                     /locus_tag="ACHKG5_003448"
     CDS             complement(6639..7493)
                     /gene="gatY"
                     /locus_tag="ACHKG5_003448"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0869484.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7792..8844)
                     /gene="fbaB"
                     /locus_tag="ACHKG5_003449"
     CDS             complement(7792..8844)
                     /gene="fbaB"
                     /locus_tag="ACHKG5_003449"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0869485.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9101..10378
                     /locus_tag="ACHKG5_003450"
     CDS             9101..10378
                     /locus_tag="ACHKG5_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0869486.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10375..11379
                     /locus_tag="ACHKG5_003451"
     CDS             10375..11379
                     /locus_tag="ACHKG5_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0869487.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            11376..12341
                     /locus_tag="ACHKG5_003452"
     CDS             11376..12341
                     /locus_tag="ACHKG5_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0869488.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12315..13061)
                     /locus_tag="ACHKG5_003453"
     CDS             complement(12315..13061)
                     /locus_tag="ACHKG5_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0869489.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13113..13931)
                     /locus_tag="ACHKG5_003454"
     CDS             complement(13113..13931)
                     /locus_tag="ACHKG5_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP0869490.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(13996..14796)
                     /gene="thiD"
                     /locus_tag="ACHKG5_003455"
     CDS             complement(13996..14796)
                     /gene="thiD"
                     /locus_tag="ACHKG5_003455"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0869491.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14793..15581)
                     /gene="thiM"
                     /locus_tag="ACHKG5_003456"
     CDS             complement(14793..15581)
                     /gene="thiM"
                     /locus_tag="ACHKG5_003456"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0869492.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15616..15712)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15915..16154
                     /locus_tag="ACHKG5_003457"
     CDS             15915..16154
                     /locus_tag="ACHKG5_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFP0869493.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         4034 a         4219 c         3917 g         4059 t
ORIGIN      
        1 ttccgggtaa ctccatggag gctttaatat attatattga agaagccact gtttagttga
       61 atattaggta tatgctcttt tttgaaattt atcggtggct gccgttagta ttcgctgtcc
      121 ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga ctatttatcc
      181 tccgactttc atcctgttct gactccacct tttcttttct gctctacact atctatagac
      241 caatcataaa ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg
      301 gcaaaagtac tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa
      361 tgacgatcca tgtgcgggtc acctgggaga aaggcgatgc cgcacgatat gtagaggagg
      421 cccggaagtt gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag
      481 tttctacggc gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat
      541 taggaaccgc caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc
      601 tgaaactggc aattgccggt aacgccattg cgatagatat ggcgcaggtc aacaaacaaa
      661 cctgttttat taatatggcg acaggcggat ttgggacgcg tattaccaca gaaacgccgg
      721 aaaaattaaa agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg
      781 atactctgca accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg
      841 ccctggtcat tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga
      901 acgcgttaat taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc
      961 cggctctcgt atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt
     1021 cgtcgtggtt tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt
     1081 tgagtgggca aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac
     1141 cagattgtcc attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa
     1201 tgccggatgc ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat
     1261 tttacccgtg tcttctgcaa tgccagttta tccacatctg acacggcatc atcggtaata
     1321 ataatgtcaa ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat
     1381 gaatcgagaa cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa
     1441 ccttcattaa aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt
     1501 aaatcggcat taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt
     1561 ttatggcgga ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca
     1621 aacgctgccg ggagactgtt ggtaatcacc gtgatatttt tggcggtcat taatccttcg
     1681 gcgataagca ttgtcgtact tccgctgtcg agaataacgg tcatcccttc gtggatcatt
     1741 ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg caagctgaaa tcgctcttta
     1801 aacccgactt cctgggtctc ggtttcacta ttaccagaca ttattttcgc cgcaccgcca
     1861 tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct
     1921 tccgaggcag caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact
     1981 aattggatga tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt
     2041 gtggctagtg taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc
     2101 cagcatcacg ctggcccgcg gtttcaggga atgagcaaca ctttgcccgg catagcatta
     2161 cgagcgatgt cacgcaccgc ctgggcgaag ctttcaaagc ttccacggtg agcgattaat
     2221 ggctccaggc ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc
     2281 ggccaagggc tggagtagtt catccaactg ccgataaccg tcagctcttt acgcaatatt
     2341 ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagtgccagt
     2401 tgggcatgag gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc
     2461 tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc
     2521 atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct
     2581 gagctaatgt cgatagccgt cacactcttt gctcccagcg cgacagcgca ctgaatagcc
     2641 agcaggccaa tggttccggc accaataata ataacgtttt tattctcaca accttgcgct
     2701 aaatgaaaag catgcaggcc aacggtaatc ggctcaataa aagccccatc ctcaataggc
     2761 atatccgtgg gtagagcaaa gacatttttt cgcttaacga caatatattc ggcaaatcca
     2821 ccatcacgcc gtgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaaa
     2881 cactctggac aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccagggtgt
     2941 aaatcatcaa caccggatcc taccgcatca atatagccgc taaattcatg gcctaacgtt
     3001 attggataat aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc
     3061 gagctggcaa tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgctttct
     3121 gcaacgcgca cgataccatc agtatcattc accactgatt tcataaaaac tcctaattgt
     3181 taagggggat aacctccccc tcaggtaaaa attattctgc gagaacgact ttctcttgtt
     3241 taataaagcc acgcgctcta cgccaggtca tgaaaatacc ggtaagataa attgcgccga
     3301 taataatgaa accgggaata ttttgcgggg agaaaacctg aatcagtaac caggtaatgg
     3361 gagaaccgcc ctgatccatt gaagccacca tacccccggc ttttaacgcc ccagcattag
     3421 ccgccagttg ggtgtgcaaa ccaatagttt gcgtcgcgat ccacagggtg atgctcataa
     3481 tgatgacacc cgagattaag gtgcggaaca gatttccacg atgcaccgcg accgccatcg
     3541 ccacgaagaa gccgatagtg gcaagatcgc caaacggcag tacctgatta ccaggaacac
     3601 aaacagcaat taaaatggtg agcggaataa aaatcaggct tgccgatacc accgccgtat
     3661 gccccagcag taatgcggga tccaggccaa tcaggaactc ctgaccgccg aacttcgcct
     3721 gtaaacgact acgggcctgc ttagcgatgg gcgttaaacc atccatgatg ggtttaatca
     3781 cccgtggcat tagcagcatc accgccgccg tttttaccgc cagctgcaat acacctttga
     3841 catcgtaacc cgcgaggatg ccgataatca gccccatcac aaaacccacg gtgacaggct
     3901 cgccaaacgg gccaaatttg cgctgaatat cgtcggcgct aaatttaata cggttaacgc
     3961 ctgggatttt ctcgatgata gcatcgacca gcaccgcaat cggccccata tacgccgacg
     4021 taccgtgcgg aatagcaatg ccttccagct caaagaaatt tcgggtatcg cgggcaaacc
     4081 agtcgccgag cttataaaca aacgccgcgt gaattaccac acctgccatc cctatcatcc
     4141 atgaaccggt tgccagatgc agcaacgcgc cggtgaaggt catatgccag atattccaga
     4201 tatcaacatt taccacccgc gtcatacggg tcagtaacat cgccacgtta accagaatcg
     4261 caatcggaat cgccaccagc gcaatttgcg acgcccaggt cattggtgaa gagcccggcc
     4321 agccgacatc gaccacatgc agattcaggt cgaaattttc cgccatcgct ttcgccgccg
     4381 ggccaatgga atccagcatt aagccaatca caaggccaat gccaacaaag ccaatcccga
     4441 tatgcagacc cgctttaaag caatcgcctg ccttcatgcc taaaatttta gaaaaaataa
     4501 tgatgacaat cggcaacatc accgtagggc cgagatcgag aatataacgc atgacttctg
     4561 aaaacatagg tcacccctgt aagatagtca gaattttatt ttgtaatgct tcgataccga
     4621 caccagaaac aaaaggcatg ccgtgaacta acggaatatc gccaaaacta cgatccaccc
     4681 tggcagtggt gcatatcaaa tgcacgccat ccatataggt ttctatttca ttaacccgac
     4741 actggattaa ttcaacagga atattatgac tctgacacaa ctctttaatt tcttccgccg
     4801 ccatcgtaga ggtcgcaacc gcgcctccgc aagcgacaat aatcttgcgt ttcataaata
     4861 gtccttttta ttgttacaga gggatttatt cgtctgaatc taaaacatac tttgtgaagt
     4921 attcctttaa ctgagtttca ggaagagtga ttagtgtctc aacgatatcg ggctgttgta
     4981 acttgccaaa taaacagcgt aaaagtttca attgctgctg cggattttcc acaatcaacg
     5041 caataaccaa cgataccgcc acgtcattat catcatccgc ttgctgaaaa tgaactttat
     5101 ttgttggcct taacagataa atggctgacg acttagcatg aatcgcctca caatgcggta
     5161 ttgcaatggc gtgctgctca agcattatcc cggtagggaa ttctgcttct ctggcaatta
     5221 atgcctgtgg ccaggtatca tgaaccacac ctttggcgag catctcatta ccgatatggg
     5281 ttaaaacttc gctacgatcg acaaaagaaa ttccgctacg aacaaacagg ttagtcatat
     5341 accgtcctta ttccgcacag ccgtagcgat aggcgcgcaa aacatcataa attttatcca
     5401 taatcagctg atgcggtatt gctgataatt ccccggactg aatgcgttca aattgtttgg
     5461 gaagatactg actaatcatg cccagtggga tgtccacgcc ttgcagattc accatcatcg
     5521 tttcaacgct atttttaatc cgactatgcg gccagtaata acgaatacga tccgacaggc
     5581 tgtaacgaat atccagtaat gaatcgttaa aacccgtgcg ataatatttt ttccagtatt
     5641 gcggttcgtc gagcatcact tcttcaatta ccgccaggca accgctgcga ttttcggggg
     5701 cgataagttc ctgctcaatt tgcgccagcg caaatatcgc ctcacgtaaa gcaaaggtta
     5761 atgcgggacc gactttcaat attgcaaagt gatcgcggac taattcccaa taagccgtcc
     5821 gggtctggta atcggtagaa tgtgcttcat aaaccattcg ggtattttct atccattgcg
     5881 ccagcgcctg cgcttcctgc ggctgataat ggataatatt gctgtgatca aattccacac
     5941 ccggctgcac cacgatggca atcacgcgtg ttaatgcctc tgtcagccca cgggcaataa
     6001 aggccttttg atgcgtacgt aaagtattgg cggcatcttc aacatgggtg atgtgtactg
     6061 actgaatggc gctggcctca ccgcccggaa ccggaacttc ggtgccaatg acatagctca
     6121 gttgctcacg ctggcaatct gtcgccacac tttccgcagc aaagcaaagc acagcagctc
     6181 gttccgcaac cgtttctggt gctaacggta tgggatcccc cgcgcaggac attgacgcat
     6241 caagatgaat tttactgaag ccagcacgaa catatgcctt taccagctcg acggattttt
     6301 ccatcgccgc atccacattt tcttgctgcc agcagtttgg ccccagatgg tcaccgccga
     6361 gaataatacg ctcgcgtgca aatccgactt tatcggcaat cgcaaaaaca aattcgcgaa
     6421 agtctgccgg tgtcattccg gtataaccgc caaattgatt gacctggttt gacgttgctt
     6481 caatcagcac tttgcgcgtg ctgttgcgat caaatgccag cgccgcttcg ataaccaacg
     6541 gatgggcaga acagactgaa catatgccga tatgttcacc agctttatgc cgggcaatta
     6601 acgttttcat gttttttcct tgttaaatgg caggtgcgtt atgccctgcc ctcgcagcca
     6661 caatcggcaa tcactttgct caccacatcg cgcattgcgg atttagccga ctgcaaataa
     6721 tcccggggat cggtcgcttc ggggtgctcg gtcaggtaat tttttaacgc ctgcgagaag
     6781 gcatttttca gctccgttgc aacgttgatt ttgcatatcc ccagtttgat ggtttgctga
     6841 atatccttag tcgataaccc tgacgcgcca tgcagcacca gcggtaagtt cacccactgg
     6901 cgaatgttct ccagtcgaga aaaatcaagc gccggtgcgc tggcatacat accatgagcc
     6961 gtgccgatgg cgaccgccag ggaatcaatt ccggttgcct cggcaaattc acgcgcctga
     7021 gcggggttgg tataaaaagc atcggcttca ttgacttgca catcatcttc ctggccgcca
     7081 agttgcccca gctccgcttc gacgctgaca tcaaagcgat ggcaaaaatc caccacctct
     7141 ttgacccgtg aaatattttg cgcaaaaggc aaatgcgagg cgtcaatcat gactgagcgc
     7201 acgccagaac gaaccttctg agcgatatcg tcaaatttcg tgtgatggtc gagatgaatc
     7261 gccagtggat ggtgataatg cttcgccatc gcgctgacca gcgccaacag attttctgta
     7321 ccagcatgag taaatgtgcc aggcgttccg gcgatgatga ccggcgcatg caggttggca
     7381 gcggtttcta ccaccacttg catcgtttcg agattgtgaa tattgaatgc cggaaccgcg
     7441 taaccgccgc gctgcgcgtt gttcagcatc tgctttgtcg ataccacgta cattttcaca
     7501 tcctgtcgat tgctttcgat ttcaaaacat aatgaaatca tttgttttta aatgtcgaga
     7561 taacgatcac aaaaacgaca atatgaaaat taatagagga atgaaaggca aaaaaaacgg
     7621 cctcccggag ggaagccgca acaaagtgcg cgtggtttta tgcaggatgc ggcgtaaaca
     7681 tatgtggcag cgctgcgtag gcatgataag acgcttcagc gtcgcatcag gcataggttg
     7741 ccggatgcgg cgtaaacgcc ttatccgtcc tacggggtag tgccgttcgt atcaggcgat
     7801 agtaattttg ctatcgagat aaacgtcctg cacggcgtta atcagtttca cgccgtcagc
     7861 catcgatttc ttgaacgcct tacgtccaag aatcagcccc attccgccag cgcgtttgtt
     7921 gataaccgca gtacgcactg catcactgag gtcagtttca ccgcctgcag caccaccgga
     7981 gtttatcaac ccggcccggc ccatatagca gttagctaac tgataacgca ccagatcaat
     8041 cgggttttcg ctggttaact tgctgtacac gcgatcgtcg gtataaccgt aattaattgc
     8101 tttatagccg ccgttatttt ccgccatttt ttgtttgacg atatctgcac ctatggttgc
     8161 cgccagatgg ttcgcctgac cagtcaggtc ggcggagaca tggtaatcaa cgccatcttt
     8221 cttaaaggcg gagttacgca aataggccca cagcactgtc accatgccca gctcgtgcgc
     8281 acgttcaaaa gccgcagaaa tttcttcaat ctggcgacgt gactcttccg agccaaaata
     8341 gatagtcgca ccaaccgcca ccgcgcccat gttgaaggcc tgctccacgc tggcatacag
     8401 cgtttgatcg taggtattcg ggtaacttag cgtctcgttg tgattaagtt ttacgagaaa
     8461 tggaatgcga tgcgcatagc gccgcgatac cgacgccaac acgccgtaag ttgatgccac
     8521 acagttacag cccgcttcga tcgccagttc aacaatgttt ttcgggtcaa agtagagcgg
     8581 gttagcagca aatgaagctc cggcagagtg ctcaacgccc tggtcaaccg gcagaataga
     8641 aagatatcct gtgccagcca gacgcccggt gttgtacaac gtctgcatat tacgtaacac
     8701 cgctggcggg cgattattgt caatcatcac gcggtctacg tagtcatgtc cggggagata
     8761 aagctggtca gaaggaatag tcatacaacg gtgctgtaaa aggttgtcgg cgtctttgcc
     8821 aagcaactgc gcaatatctg tcattactat gctcccgtaa attccgattg gatatcggct
     8881 atggattgtc ctggcccgcc ttttgcgggc aatcataatc ctggtcgtta cgggcgagtt
     8941 tttccatctt ttggatgttt tttcagcgtt ttctgttggc tcgattcatc agaaaaaatg
     9001 ttagcgcggt caagttttca ccgcaaaggt atttaaaagg tattattaag tggtattgtc
     9061 atcgcgtacc ttacattacc tgtcatgaag gaattaaaag atgaaaacaa caacaaagct
     9121 gtcgttcatg atgtttgttg aatggtttat ctggggcgcg tggtttgtgc cattgtggct
     9181 gtggttaagt aaaagcggtt ttagtgccgg agaaattggc tggtcgtatg cctgcacagc
     9241 cattgcggcg atcctgtcgc caattctggt tggctccatc actgaccgct ttttctcggc
     9301 gcagaaagtg ctggcggtat tgatgttcac tggtgcggcg ctgatgtatt tcgctgcgca
     9361 acagaccact tttgccgggt tcttcccgtt actgctggcc tactcgctaa cctatatgcc
     9421 gaccattgcg ctgactaaca gcatcgcttt tgccaacgtg ccggatgtgg agcgtgattt
     9481 cccgcgcatt cgtgtgatgg gcactatcgg ctggattgcc tctggtctgg catgtggttt
     9541 cttgccgcaa atgctggggt atgccgatat ctcaccgact aacatcccgc tgctgattac
     9601 cgccggaagt tctgctctgc tcggtgtgtt tgcgtttttc ctgcccgaca cgccgccaaa
     9661 aagcaccggc aaaatggaca ttaaagtcat gctcggcctg gatgcgctga tcctgctgcg
     9721 cgataaaaac ttcctcgtct ttttcttctg ttcattcctg tttgcgatgc cactggcgtt
     9781 ctattacatc tttgccaacg gttatctgac cgaagttggc atgaaaaacg ccaccggctg
     9841 gatgacgctc ggccagttct ctgaaatatt ctttatgctg gcattgccgt ttttcaccaa
     9901 acgctttggt atcaaaaagg tattattgct tggtctggtc accgctgcga tccgctatgg
     9961 cttctttatt tacggtagtg cggatgaata tttcacctac gcgttactgt tcctcggcat
    10021 tttgctgcac ggcgtaagtt acgattttta ctacgttacc gcttacatct atgtcgataa
    10081 aaaagccccc gtgcatatgc gtaccgctgc gcagggactg atcacgctct gctgccaggg
    10141 cttcggcagt ttgctcggct atcgtcttgg cggtgtgatg atggaaaaga tgttcgctta
    10201 tcaggaaccg gtaaacggac tgactttcaa ctggtccggg atgtggactt ttggcgcggt
    10261 gatgattgcc attatcgccg tgctgttcat gatctttttc cgcgaatccg acaacgaaat
    10321 tacggctatc aaggtcgatg atcgcgatat tgcgttgaca caaggggaag ttaaatgaaa
    10381 acagaacgta ttctcggtgc tctttatggg caggcgttag gggatgcgat ggggatgccc
    10441 tccgagcttt ggccacgcag ccgcgttaaa gcacactttg gctggattga ccgttttctg
    10501 cctggaccaa aggagaacaa cgcggcctgt tattttaacc gcgccgaatt caccgacgat
    10561 acctcgatgg cgctgtgtct ggcggatgcg ttactggaac gtgaaggcaa gatcgacccg
    10621 aatctgattg ggcgtaatat tctcgactgg gcgctgcgtt tcgacgcctt taacaaaaac
    10681 gtactaggtc cgacctcgaa gattgcgctt aacgccattc gcgacggtaa acccgttgct
    10741 gaactggaaa acaacggcgt gaccaacggc gcagcgatgc gcgtctcgcc attaggttgt
    10801 ttgcttccgg cgcgtgatgt tgattccttt attaatgatg tggcgctggc gtcgagcccg
    10861 acccataaat ccgatctggc ggttgcgggc gcggtagtca tcgcatgggc gatttctcgt
    10921 gctattgacg gagaaagctg gtcagcgatt gttgattcat tgccttcaat tgcgcgacat
    10981 gcacagcaaa aacgcaccac cacgttcagc gcctcactgg cagcgcggct ggagattgcg
    11041 ctgaaaattg tgcgcaatgc cgacggcact gaatccgcca gcgaacagct ttaccaggtc
    11101 gttggcgcag gtaccagcac tattgagtcc gttccgtgcg ccattgcgct ggttgaactg
    11161 gcacaaaccg acccgaatcg ctgcgccgtc ctgtgcgcta accttggcgg cgatacagac
    11221 accatcggtg ctatggcgac ggcaatctgc ggcgcgttgc atggcattaa cgctatcgat
    11281 cctgcgttaa aggcggagtt ggatgcggta aatcagcttg atttcaaccg ctatgccaca
    11341 gcgctggcga aatatcgtca acaacgggag gcggcatgag cggcgctcga ttacacacgc
    11401 tgctgcctga attaaccacg cgtcagccgg ttatggtgat tggcgcggca gtcattgatg
    11461 tgatcgccga cgcttatgcc ctcccctggc gtgggtgcga tatcgaactg aaacagcaga
    11521 gcgttaacgt tggcggctgc gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg
    11581 aagcgggtaa cgccttgccg ctcggtcagg gcgtgtgggc ggagattatt cgcaaccgga
    11641 tggcaaaaga gggcttaatc agcctgatcg ataacgccga aggtgataac ggctggtgtc
    11701 tggcgctggt tgagccggat ggcgaacgca cttttatgtc attcagcggt gtggaaaatc
    11761 agtggaatcg ccagtggctg gcgcgattaa ccgttgcgcc tggcagcctg ctctattttt
    11821 ccggttatca actggcctcg ccctgcggcg aattattagt ggaatggctg gaaaagctgc
    11881 aagacgtgac gccgtttatc gattttggcc cacgtattgg cgatatcccg gaggcattac
    11941 tggcgcggat catggcctgt cgacctttag tgtcgctcaa tcgtcaggag gctgagattg
    12001 ccgccgaacg ttttgcttta tccgcagaga taacaacact tggcaagcaa tggcaggaga
    12061 aatttgccac gccgttgatc attcgtctcg ataaagaggg cgcatggtat ttcagcaacg
    12121 acgcttctgg ctgcattccg gcatttccca cgcaagttgt agacaccatt ggggcgggcg
    12181 acagtcatgc aggtggcgta cttgccgggt tggcctctgg tctgccactg gcggatgccg
    12241 tattactggg caatgcagtg gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc
    12301 caacgcgcga ggaactactc ctcgcacaca aaaacgtata gatcgctgcg acagtggcta
    12361 atgctgtact caataggccg ttgctgttgg tcaagcgcca cttgcttgat caccagcacc
    12421 ggtattttgc tatctaactg aatatgtgac tgaaactcgg catctggcat ccgggcgcta
    12481 acgcgggaac gtgtacgttg tgggtaaatg tgctggctgc ggaaatagtc ataaagagaa
    12541 attccgatgg catcaacatc atgaattaaa tgcgccggaa cccacgattc ctcaatcgat
    12601 accgcttctt catcgacata gcgaatacgc ttaagcaaaa agacatcact tcccgcttcg
    12661 accgccagct gctgcgcgac ttcttcggga catttcacga cacgcttgtt aacccatagc
    12721 gtatcgggct ttttcccgcg caataccacc tgctgagaaa aacctcgcgc ttctttcaac
    12781 gagtattcga agatgttgtt gatctgcgtg ccgtaaccgc gcgaacgcgt caccacaccc
    12841 tcttcttcca gcgcctgcat cgccttgcgc accgtaatgc gtgaaacgcc ggttaactga
    12901 cttaaatcac gctcaccggg caaaatattg ccatgctcca gcaccccgct gcgcacggca
    12961 ttttttaccg tttcggcaaa cttaatataa agcggcgtgt tatccgccgc taaaatacgc
    13021 tcgttcagtt gggcaatcag ctgggtatgc gcttgttcca tttgtctttt tcctgccagg
    13081 gagtctggtg ggagtatatc actgcttctt atttaaggcg tttctttaat cccatcaacg
    13141 aatgcctgca actcctcctc tgtgccatta aacacattaa aatccaccga accattaatg
    13201 ccatctacct gcccacggtc ggaatgctgc cagaaacgcc aggccatgcc gtcattgtcc
    13261 gggcgacgtt gatagtagtg agccacccac cacggatatt cattgaaata gcccgccaga
    13321 ttggtgtgat aaaaaacggc tcctgagtaa ataaccggct tttttcccgt acttttttcg
    13381 accattttta gccactgact tacccgcttg cgtaattctt ttgccgataa ttttccccgt
    13441 tcttctacgt ccagcacggc agggaaatcg ccttgtgaga aatccaccgt ttgcagaaat
    13501 aatcgcgcct gaacaggcgc tgctaccgac ggggagaaat aatgatacgc cccgcgcagc
    13561 aggccatttt cgcggcttag ttgccagtta cgcgaaaaat aggggtccac cagcttttcg
    13621 ccttccgtcg ccttgataaa ggcaaattgt aagcggatac cgttatcgcg catttttgcc
    13681 acccgctgcc agtcgatccg ctcctgccag cgcgaaacat ctattccgtg aatggtgtaa
    13741 ctggcaggaa tacgaatagc gaaagattta accgggcgat aaccgtagaa gtggatgtag
    13801 tctgtaatct gaactgctgt cgaatagaaa aaattcaccg tctgacgcgg ataaattgca
    13861 acgatggaaa ttagcccaag agcgcataat agagcggtga gtttttttcg actggtaatt
    13921 cttagctgca ttggtttcat ccctgaatgt cagtgccaaa ggctgacaat aaccaaagca
    13981 gctatagtac ggtgctcacc accaggcgtg gaagtggtga accggaccaa taccgtgacc
    14041 aacttccagc gtgtcggcct gggctaacgc cgatgaaagc cagctttttg cctcctgtac
    14101 ggtgtcagcc cagtttgtat ggcgcgggcg tagtgcagcc aacgccgcag agagtgtaca
    14161 accagtgccg tgggtgtttt tggtcataat gcgcggtgcg gtaaaccgtt gttcaccctc
    14221 gcgggtaaac agccagtccg ggctttgctc atcatccaga tggccacctt tcattagcac
    14281 tgcgccacag cccatcgcca acagcgctcg cccttgttcc agcatttcct gttcggtgcg
    14341 cgcgtgtggc gcgtcgagca aggcagcagc ttcgggcaag tttggcgtta ttaatgaaac
    14401 ctgtggcaat aatcgactgc gtagcgtggc aactgccgaa ggtgaaagca gtgggtcgcc
    14461 actttttgcc agcataacgg tgtcgagtac cacgttttgg atctgataac gttgcaaccg
    14521 ttctgccacc gcttcaacaa tatcggtttc cgccaacata ccgattttag tggtatcgat
    14581 tcgcacatcg ctgaacaccg aatcgagctg ggcggcgaca aaatcaggct caatgcgata
    14641 caccgactgt acgccacggg tattttgcgc caccagtgca gtaataactg agcaaccata
    14701 agcgccaagt gccgagaagg ttttaagatc ggcctgaatc cccgcaccac cactcggatc
    14761 agtaccggca atcgtcagag cgttaattcg tttcatgcct gcacctcctg cgtcaattgc
    14821 cagagcgcat caaggaaatg tggaacaaaa ctgcctggcc cctcgcttct ggcgactgcg
    14881 cgttctccgg cttgtttcat ccagtgacag gcagatgcga cattttccag catatcgcct
    14941 ggtaacgcac aacaggcagc gacaaccgcc gataatgcac agccagttcc taccactttg
    15001 gtcattaacg gatcgccacc gtgaatgcca acggcgcgat ggccatcggt aacataatcc
    15061 acctcgccag tgaccacgac gattgcgcca gtttcccgtg ccagtgtttg tgcagcgggt
    15121 atcgcgttaa ctgcggcgtc agtggtatcc actccccgtc caccattagc aacgccagct
    15181 aatgccatga tttccgaagc attaccacgt atcgctgccg gtttaaaaga taaaagttca
    15241 tgacaaaaat ggcggcgata atcgagcgca ccaaccgcta ctggatcaag cgtccagggt
    15301 gtttgagagc tttttgcttg ctcaacggca gcacgcatcg cctgtgcgcg tggctgcgtc
    15361 agtgtgccaa cgttaatcaa caaggcactg gcgatagccg caaactgact ggcctcttcg
    15421 gtttcgataa ccatcgctgg cgatgcaccg agcgccagca aggtattggc agtaaaggtt
    15481 tgcaccacat cgttggtcat gcagtgcaca agaggggaat gttggtgaaa aaggtgtaac
    15541 gtgtgcgcag attgcgcgga actcagcagg tcgacttgca tagtttgctc ctgccataac
    15601 gtgaagaagc aatgacctgg tggtccgtga cttccctacg ctggcattat ccagatcagg
    15661 tgatacgggt atttctcagc cttcacgcag aagggcaccc cgagtcgttt ggttgaatct
    15721 ctatattttg tgccagctta ggggaagagg cttgcagatg caagctcgcc tccattcatt
    15781 acgtcttcac tgtgtacccc atatcagtaa tgaaaaagaa acaattcaca cccatcattc
    15841 aggtggtaaa aactgtagac tatcttaatt agctaattta tattatttat ctaaatttga
    15901 taggtgagat aaaaatgaca gaaacccaga tctacgagaa cattaagcaa gccattagca
    15961 gcgcaccacg aaatagccaa acgatggaaa tgcacctcca gatgatcaaa tacgctgacc
    16021 atctgaaaaa cgtcacggca aaagagtttt gcgaaggtgt aggacttaaa gccagctttg
    16081 ctacagagtt cagcaaaatg agaaatctta ccgagcgact caaagccgcc ggacttgata
    16141 ccacgaagtt ataacaaacg aaatcatcaa tatattactt tcgttttcca gggttccctg
    16201 tatatgtacc tatgatcata aaaaccagt
//
LOCUS       DAZGXS010000103        16083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGXS010000103 DAZGXS010000000
VERSION     DAZGXS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..815
                     /locus_tag="ACHKG5_003458"
     CDS             <1..815
                     /locus_tag="ACHKG5_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0869494.1"
                     /translation="NVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIYLLPLAR
                     GNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHKG5_003459"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHKG5_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0869495.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /locus_tag="ACHKG5_003460"
     CDS             complement(1944..3404)
                     /locus_tag="ACHKG5_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0869496.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHKG5_003461"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHKG5_003461"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0869497.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKYIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHKG5_003462"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHKG5_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0869498.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6526..7428)
                     /gene="fimH"
                     /locus_tag="ACHKG5_003463"
     CDS             complement(6526..7428)
                     /gene="fimH"
                     /locus_tag="ACHKG5_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0869499.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7448..7951)
                     /gene="fimG"
                     /locus_tag="ACHKG5_003464"
     CDS             complement(7448..7951)
                     /gene="fimG"
                     /locus_tag="ACHKG5_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP0869500.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7964..8494)
                     /gene="fimF"
                     /locus_tag="ACHKG5_003465"
     CDS             complement(7964..8494)
                     /gene="fimF"
                     /locus_tag="ACHKG5_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0869501.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8504..11140)
                     /gene="fimD"
                     /locus_tag="ACHKG5_003466"
     CDS             complement(8504..11140)
                     /gene="fimD"
                     /locus_tag="ACHKG5_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP0869502.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11207..11932)
                     /gene="fimC"
                     /locus_tag="ACHKG5_003467"
     CDS             complement(11207..11932)
                     /gene="fimC"
                     /locus_tag="ACHKG5_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0869503.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11969..12508)
                     /locus_tag="ACHKG5_003468"
     CDS             complement(11969..12508)
                     /locus_tag="ACHKG5_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0869504.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12573..13121)
                     /gene="fimA"
                     /locus_tag="ACHKG5_003469"
     CDS             complement(12573..13121)
                     /gene="fimA"
                     /locus_tag="ACHKG5_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0869505.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13586..14182)
                     /gene="fimE"
                     /locus_tag="ACHKG5_003470"
     CDS             complement(13586..14182)
                     /gene="fimE"
                     /locus_tag="ACHKG5_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP0869506.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(14660..15262)
                     /gene="fimB"
                     /locus_tag="ACHKG5_003471"
     CDS             complement(14660..15262)
                     /gene="fimB"
                     /locus_tag="ACHKG5_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0869507.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT         4103 a         4003 c         3722 g         4255 t
ORIGIN      
        1 gcaatgtcct tcagagtcaa attattacag ataatcactt ccttcatcat ccaaaagtcg
       61 aaagtgagtt aacacgtaaa tatgaacgtg cacgcctgga tacggaaaat atctatcttc
      121 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 aacttgatat tagtaaagaa tcctggccat ttaattatgt gacagaagca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgctacagag ttagcagaag
      361 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttccaggtta cgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaagaattg
      541 atatctatat tgatcagata ataactgtct caggggtagc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatccagg taccgcggag atccatgcaa aaatgggata taaagttatc cccggtgatg
      721 aagatgcgcc actcaaacgc atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaatggaat tatattggca aataaccact gagaagaatg agtactaaca
      841 ttcaggttat atagatattt aaacaatacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
      961 cgtcgacttt atccagcggc catgaatcaa acagatagcc gtcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attgggttgt
     1201 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1261 cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc gcactggaag
     1321 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcagggc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     1441 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
     1501 agctatcaag aacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg tagccgttca ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     1861 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccgc agtcaccgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     2161 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     2281 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     2461 gcaatagttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     2521 agcatacgca gtttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2581 ttatcccagt ccggacgacc attaacgaag ttatcttcaa taacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     2761 tcaatccatg ccgctagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     2821 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     2881 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     3001 tcagcgcagt agcctttttc cgtgaccgtc agggagacaa tcgccgtttg cggacgcgcc
     3061 atcgcgttga gaataccttc gcagccgtcg atttccgggt gcagcgcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctttccgcg cctttttccg ccacggtgta cagcagttgc
     3181 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 ataccccagt cgctgtcggt gctttccagc agatgatggg tgtacagcgc ctggtgcgca
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaga gagtgacggc aaggaagaag gagccggcac ggcgcgcagc catgccggtg
     3481 ggtattaatg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3541 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac caaatcgttg tcagcacgca
     3841 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaacagcc atacggacgc caacttcttc ggcaaccgga ataatcgctt
     4021 tcaggaagac agcaaagttt tcgcgcagtt tggctttgtc gatatatttg tacagctcca
     4081 ggtgcttacg gaactggtcg agggtgtacc cttcttccgc acctggcaga ccagcgatga
     4141 tgttacgggt cagacgcgct ttgtcttcat cgctcatagt ggcgaagcgt tcagctgcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     4501 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa cccgaattct gaaattggtt aaccacatca caagaatttc
     4861 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4981 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5041 cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5161 caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cgcatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     5341 tggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgtcat
     5401 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5521 cgcagatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaacaa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5761 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg cgatgtttat
     5821 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ctgaagcaaa ctatcatcga caccggcatt ggcgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
     6061 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatccttgat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtgctggc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaacgat gcatctttct ggttgttcaa
     6241 aggttacttt gacctgtcgg taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
     6361 cagagcctgc caatggcagg ctcagttcgg attgtcggca aagtgcgctt tgtttatgcc
     6421 agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gtttttagct
     6481 tcaggtaata ttgcgtacct gcattagcaa tgccctgtga tttctttatt gataaacaaa
     6541 agtcacgcca ataatcgatt gcacattccc tgcagtcacc tgcccgccgg tacgtgcgta
     6601 atttgccgtt aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt
     6661 attcgctgga ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga
     6721 aaacgacgcg gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta
     6781 ataccccagg ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg
     6841 gtagtccggc agagtaacgg tgacatcacg agcagaaaca tcgcagccgc cagtaggcac
     6901 taccacatca ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt
     6961 gttggtctgt cgcaaaataa gcacggcaat taatgagcca gctttaatcg ccaccccgcc
     7021 cgcactgctc acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt
     7081 ataaacaacc cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt
     7141 tacggtcccg gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac
     7201 atagtctgta atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac
     7261 gaccaggttt tgccccacat tcacggcagg cgcaaggttt acataaacat tagcgctgcc
     7321 accgccaata gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac
     7381 cgaccagccc atcagcagta cagcaaacag ggtaataact cgtttcatta caattatctc
     7441 ttcgggttca gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg
     7501 ctccgccatt taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat
     7561 catccacctg aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta
     7621 gctctaactg gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg
     7681 ccccgctgaa gctggcagtg acccttgacg ttcccaccgg acaattagtc aactcaagcg
     7741 caacatcatg ccaggccgat gccgccccgg cagacatcag actgaaagaa taaagatcgc
     7801 cgagatcaac cgtggcattg gtggtggaaa ctgtgcacgg tttggcgacg accttaccgt
     7861 tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg
     7921 ccaataaata cccacgtttg cgccatttca tgagcatctc cagttactga tattcaagag
     7981 tgaaggtagc cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc
     8041 gggcgtaaaa attcaacgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcgg
     8101 acgatggagc attaaggggt atctgatttt gctgctcatt cagaagctgt attcccagtc
     8161 ccgaagccgc tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa
     8221 cgccggtaaa cccaactttt acggcagaaa cggcgttacc acagggtgac agcaaaatac
     8281 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
     8341 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
     8401 agccgcggat agtaatcgtg ctatccgcag ccaaagcgtg actcactgcc agccacaaaa
     8461 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
     8521 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacaat
     8581 gagcattttc ctcttctccc catttcacct gaacttttcc cgctagaggc attccgctga
     8641 ggtaaacctg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
     8701 tcgccccaaa cggcagcggc ttattattgt gggttagcgt catgagcagt tttatcccaa
     8761 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
     8821 cgttatctaa atcgacgtta tcagccaggg tattggtatc cagcgccact ctattttccc
     8881 gatattcagt ggcataaggc agcacggcat aaccgcgcca gtcggtacgc acccccgtct
     8941 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
     9001 acggctgccc cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat
     9061 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
     9121 gataattcag cgtggcgtag cctgtgcttc cgctattacc atcgcctccc ccggcatagc
     9181 cggtttgcac gctatagctg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
     9241 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
     9301 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttacgcgcta
     9361 acatctgatc acgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
     9421 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
     9481 cccaataagt ttgatggcta ccactcaaat acagtgttga tgagcgcccg agttgctgag
     9541 taacggtgag ttgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
     9601 tcggcttaac ctgaataact ccgtcctgtg tttcgatgtt gtagccattc attcgactgt
     9661 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
     9721 tattcgtgcc tgactcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
     9781 gctgactgtc atcgggaagt gtggaattag cctgagtcat atccacagac agagcgccca
     9841 gtgcccccat atttttcccg ataccaaaat taaaagcacg ataacgatct gccagttgcg
     9901 ttccaccata tattgtccag ccagctggaa ggccgtggag taatgtactt tggaaaaagc
     9961 ggggtttttc ctgttgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag
    10021 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
    10081 tgccgtcagc ctctttaatc gttacctgca agtcaccact attacctgcg gcatagatat
    10141 cgttgatggt aaaaggcccc ggcggcaccg tactattata aatgtcatac ccattttgtt
    10201 taatagtgac ctgtgcagta ccacgagcaa taccgtggat caccggggca aatcctcttt
    10261 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
    10321 cgaaaatatc accctgagta taaccatcac ccagcgtcag ccgggaacgt aacggtatta
    10381 tgtctcgctc aagccaggta ttgatatgct gccatttatt tttgctacct gatgatctgt
    10441 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
    10501 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat
    10561 ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat aactcaggag
    10621 gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct
    10681 gctgaccaac atctaaatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg
    10741 catcatccgc cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga
    10801 gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga
    10861 catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg
    10921 gtaattcttg cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta
    10981 aaaagcgcgg attaaaatag agttcggcag atgacaaagg tgcctgtacg gcaaaagcac
    11041 aggcgacaaa gagccggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt
    11101 gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc
    11161 acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc
    11221 ccgtcatttt gggggtaagt gcaccataat catttattgt tcggtaagta atattgcttc
    11281 ccgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaag gcattttcaa
    11341 gaacccgggc tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttaatca
    11401 gcgtcagaga attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca
    11461 acgctaattt agccggacga tagtacagtt taatgcggct gataattgcg agctgtagcg
    11521 tattctcagt caattttgat ttatccattg acggaatcgc tttaacgttc atccagaata
    11581 aactttcccg gtcctgtggc aattggttat ttgttgcatc aagaatacgt aaggtattct
    11641 ctttttttcc cttcatcgca aacagaggag gcgtcacgat aaaacgacca tcctttacac
    11701 catcggcatt ttccacccat gattgaatta aataggtact attttcatca ttatttgtca
    11761 cggcaagttg cacttgtttt tgccctgccg gataaattat gcgagtcgca cctaaggcca
    11821 ctcccgcttc agcgcgtccg gcaaccatca ttgccatgaa catcaggata cctgccagca
    11881 agcagaatgt tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt
    11941 tcctgttatc acattatctg ctgaacaatt actgataggt taaagagaac caggcctgtg
    12001 cattggcgat gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta
    12061 gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg aggacgatta atcggtacga
    12121 ggtttccttc atcatcaaac aacgctacgc caatattggt ggctatccct ggcccctctc
    12181 ccacggaaag cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacccacac
    12241 gttcactcac caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgcgctat
    12301 cttcccctac cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt
    12361 tatcaccggc ttcaatcagg caagtttccg caataatgac gccctgaaat tgcatatttc
    12421 cgccgggcgg cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg
    12481 ctaatagaaa taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc
    12541 tgcccgtaat gacgtccctg aacctgggta ggttattgat actgaacctt gaaggtcgca
    12601 tccgcattag cagcacccgg ggttgcctcg ccgattgcat aataacgcgc ctggaacgga
    12661 atggtgttag taccgttatt cagggttgtt tgctcactga atgtcgcacc atccagcgtc
    12721 agcgcagcac ccgttctgtc caggatctgc acaccaacgt ttgttgcgct acccgcagct
    12781 gaactctgca gagccagaac gttggtatga cccgcatcaa tcgccgtacc taaaaaggca
    12841 acagcggctt tagatgcaac attggtatcg caatcattca gctgaatgtt aaaaccgaca
    12901 gcagaactgg ttgctccgtc ctgtgccagc gatgcggtac gaacctgtcc taactgaacg
    12961 gtttgatcaa cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag
    13021 tgaacggtcc caccattaac cgtcgtggca gcggccagag ccgctgtaga actgagggac
    13081 agagccgaca gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttcaaaaa
    13141 actatttcta aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga
    13201 ctgccaacac tgcacagttt tcccccaaaa gatgaaacat ttggggccat tttgactcat
    13261 agaggaaagc atcgcgcaca aactttttca gtttatttgc tggattaata ttctattgtt
    13321 atctttattt atagattttt atattgcatg aggtggtttt tggagagaag aatgaggaag
    13381 atgcgtcgag ccacagaaac gttagcttta catatagcgg aggtgatgtg aaattaattt
    13441 acaagagaaa taatttacat atcaaacagt tagtttaaaa tttttatgct tgagaaaaaa
    13501 tacgtaactt atttatgata tggacagttt ggccccaatt gtcttgtatt tatttgtttt
    13561 ttaactttat tatcaataag ttaaatcaaa cttcttctct ttttaatttt tcgtttatga
    13621 gattatttct ttcccataat ccggcaaaac gagcagcatt actggcggta taacgtacag
    13681 tatggcgaat atttcgatgc ccgagataat cctgaattaa acgagtatct gcaccacgct
    13741 ccgccagttc ataaccgcaa gcatgcctta acatatgagg atgagtctgc gttacggttc
    13801 cagcttcaat accggcatca cgaataatgc gataggcctg ctggcgagaa agccgactcc
    13861 cgcggcgaga aataaatatg gcgtcggtcc ggtcagcgcc tttccagtta gcacgttcta
    13921 gggtccagcg ttccacagct tcacgctcat caaaacgtaa cgggtgaacg gtagaaaatc
    13981 cgttcttcag tcggcgaata tttattcggc cttcattaag gtcaagatcc tgataatgca
    14041 gatcaagcag ttcactaata cgcatcccat gccgatatgc caacagaata agacaataat
    14101 ctctggctcc cgttgccccg taacaaaccg cctgcatcat ggcctgaact tctttaccgg
    14161 taagataacg acgtttactc acaatagtag tactcctaac tgagatatat ttgaatgaat
    14221 acctatagga aacctcaatc ggtcaaaatt agcccaatag ataaaaagat aaccacacac
    14281 gaagtgatgt ggctatcagt caattacatt aaatatcata caaaaataaa atatcactga
    14341 tgtgataaca tctattttca ttgatttgat tcaatataac aatagattgc agtggattaa
    14401 cttccagttg cttaaaaaat aaaggaaaaa gtgcagaaac aggagagttt aagtgatgtg
    14461 taaatttatg aatgatttta tttatttatt ttgaagaaca taaaccattg tattttgtat
    14521 ggatatggtt aacttaatgt ttattatgat aattaagaat catacttaca ataacatcaa
    14581 ttaataaatg gttaatcatt tttgatatat cgtaagaata atgtagtttt taacaccatc
    14641 cctggtatct caactatctc tataaaacag cgtgacgctg tcgtcctctg gctctatccc
    14701 agatgccgta aaaacgccct gcattgctgg cagtatacca gacagtatga cgaatattgc
    14761 gatgcccaag ataatcctgg ataagtcgcg tatctattcc catattcgcc aaagcaaaac
    14821 cacacgaatg gcgtaacatg tgcggatgaa tctccagtga caacccggca ttaccaccgg
    14881 aagtcgagat aatatggtaa aattgttgcc gagaaagcgg attcccctta cgcgataaaa
    14941 atacccactc gctctcagca tgcggataag aagtacggat actcaaccag ttttttaaag
    15001 cctgaacttc tttgttcaat agcgggtgcg ttgttgaaaa gccttttttt aatcgatgga
    15061 tatatataca ctttgcctta agatcaatat ccgaaatcct caatcgacaa atttcactcg
    15121 cccggaaacc atgaataaaa caaagcaaag tcagacaata attacgtgct gcatgaggcc
    15181 cggtatttgc tgctttaagg agtgattcga tttcactatg ggtcaggaag ttcctttttt
    15241 tgttatcagc cttattcttc atcgtttttc ccttataatt acagacgcgc actagctgtg
    15301 ctaggttagc aataatccaa catttttatc ctgattatgt ttataaaagc gaacgtttgc
    15361 ttaataacta acgtaagtgg accagttctt ctgagtgaac ttaaatggag tagcaacagt
    15421 taattataat aacgttgcca tacaaacgct atgctcttgc atgctatgta cctttatata
    15481 tttatcaatc ggatacgaat ctaataataa ccctcatcat taatgcaact aatcttatct
    15541 atatatcatg tgatatgttc tgtaacaagt aaaatcaaca taaaaaattc agcaaaattt
    15601 aatattcgtt caactagccg tctcacatat gacattatta gtcaactcca tttgccacaa
    15661 aatggctatt tcatggaaca cagtaatttc ttagtggcta ttatcaagct aattcttagg
    15721 tccctagcga ttattcctgg ctgattcgtc agcttacgcc atgagattgt tacattcctt
    15781 tttatcctgg agatatgggg gaacgcagga gatgattttt tcatctacat tcgttggtaa
    15841 atatagcttt ggtcactacg acttaaacct tcctcaatca cagccattcg ccgggcgata
    15901 cggcaaacaa actaagtgaa acatcatcct gaaaacacca acatcaataa gcctctccag
    15961 atcgacttca gaagtgacca gttacaagcc acaaaccagg aaacatattt catgatgaga
    16021 attatgctca gcctgatggc gggtaaacac tgatttacac caagctcact gagttagctt
    16081 gca
//
LOCUS       DAZGXS010000104        15701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGXS010000104 DAZGXS010000000
VERSION     DAZGXS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..133
                     /gene="yecU"
                     /locus_tag="ACHKG5_003472"
     CDS             <1..133
                     /gene="yecU"
                     /locus_tag="ACHKG5_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0869508.1"
                     /translation="TVDGTLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            120..344
                     /gene="yecF"
                     /locus_tag="ACHKG5_003473"
     CDS             120..344
                     /gene="yecF"
                     /locus_tag="ACHKG5_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0869509.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(412..1134)
                     /gene="sdiA"
                     /locus_tag="ACHKG5_003474"
     CDS             complement(412..1134)
                     /gene="sdiA"
                     /locus_tag="ACHKG5_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0869510.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1364..2116)
                     /gene="tcyN"
                     /locus_tag="ACHKG5_003475"
     CDS             complement(1364..2116)
                     /gene="tcyN"
                     /locus_tag="ACHKG5_003475"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0869511.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2113..2781)
                     /gene="tcyL"
                     /locus_tag="ACHKG5_003476"
     CDS             complement(2113..2781)
                     /gene="tcyL"
                     /locus_tag="ACHKG5_003476"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0869512.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2796..3782)
                     /gene="dcyD"
                     /locus_tag="ACHKG5_003477"
     CDS             complement(2796..3782)
                     /gene="dcyD"
                     /locus_tag="ACHKG5_003477"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0869513.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3887..4687)
                     /gene="tcyJ"
                     /locus_tag="ACHKG5_003478"
     CDS             complement(3887..4687)
                     /gene="tcyJ"
                     /locus_tag="ACHKG5_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0869514.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4775..5326)
                     /gene="fliZ"
                     /locus_tag="ACHKG5_003479"
     CDS             complement(4775..5326)
                     /gene="fliZ"
                     /locus_tag="ACHKG5_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0869515.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5372..6091)
                     /gene="fliA"
                     /locus_tag="ACHKG5_003480"
     CDS             complement(5372..6091)
                     /gene="fliA"
                     /locus_tag="ACHKG5_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0869516.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6411..8168)
                     /locus_tag="ACHKG5_003481"
     CDS             complement(6411..8168)
                     /locus_tag="ACHKG5_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP0869517.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            8434..9831
                     /gene="fliD"
                     /locus_tag="ACHKG5_003482"
     CDS             8434..9831
                     /gene="fliD"
                     /locus_tag="ACHKG5_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0869518.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            9856..10266
                     /gene="fliS"
                     /locus_tag="ACHKG5_003483"
     CDS             9856..10266
                     /gene="fliS"
                     /locus_tag="ACHKG5_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0869519.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            10266..10631
                     /gene="fliT"
                     /locus_tag="ACHKG5_003484"
     CDS             10266..10631
                     /gene="fliT"
                     /locus_tag="ACHKG5_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0869520.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10709..12196
                     /gene="amyA"
                     /locus_tag="ACHKG5_003485"
     CDS             10709..12196
                     /gene="amyA"
                     /locus_tag="ACHKG5_003485"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0869521.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(12230..12643)
                     /gene="yedD"
                     /locus_tag="ACHKG5_003486"
     CDS             complement(12230..12643)
                     /gene="yedD"
                     /locus_tag="ACHKG5_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0869522.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12830..14035
                     /gene="yedE"
                     /locus_tag="ACHKG5_003487"
     CDS             12830..14035
                     /gene="yedE"
                     /locus_tag="ACHKG5_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0869523.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            14032..14265
                     /gene="yedF"
                     /locus_tag="ACHKG5_003488"
     CDS             14032..14265
                     /gene="yedF"
                     /locus_tag="ACHKG5_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0869524.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14374..15042
                     /gene="yedK"
                     /locus_tag="ACHKG5_003489"
     CDS             14374..15042
                     /gene="yedK"
                     /locus_tag="ACHKG5_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0869525.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            15153..15632
                     /gene="yedL"
                     /locus_tag="ACHKG5_003490"
                     /pseudo
     CDS             15153..15632
                     /gene="yedL"
                     /locus_tag="ACHKG5_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         3875 a         3995 c         3871 g         3960 t
ORIGIN      
        1 caccgttgac ggcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata
       61 ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa
      121 tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct
      181 gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg
      241 tgatgttaaa gatggaacaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat
      301 tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgat
      361 agcgtgcctt tcagccggtt tttgcatctg gcacgcagga cagaaaagag atcaaattaa
      421 gccagtagcg gccgcgtaac aggcaacctg ggtcttattt ggtgcattaa tttttttctg
      481 catgtttttc tggtggaaat tgaccgtatt ctcagagatt gacaaaatca tcgctatctc
      541 tgctgatgtc ttcccttccg ccgtccacct cagaatttct ttttcgcgct tgctgaaatt
      601 catctctggc gtcatcacta tttcatcatt taaacgcatc agagccatca gactttcgcg
      661 caccagtaac tgcattttta attgcagttc atcactaaga atgggtattt cgcgcgcgct
      721 gcaacgggaa aaggacaaaa agcccagcgc ccggtttggc agcattaaat actgagtgac
      781 accgcggcgt aaaccatgtg cgcgcgcggc ttcccataac ggctgtgctt cgttgaataa
      841 gtcatcattc cacattagat ggccctgact aaagttttca gggttcagca ccggatcaat
      901 tgcgagaaag ttttttgcct gataataact aacccacgcc tcagggtaat tggtgtagaa
      961 agccacttta ggtcgagtga atggtaccgg gtggcggaca cataacgaat agtaatcgta
     1021 ctccagctgc tgagcctgaa gctcaatttc atggtagacc tcttctgcgg tctccatcct
     1081 ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa
     1141 ccgcaacgcc cctgagagtg agtatcattt ataatgataa ttcaaactat cacataaata
     1201 agatttatat ataatttata ttattcaggc aatgaattac ttttgcaagc catcgcattc
     1261 tcttatgtta ttaatgagtt atgctgattt tttaagcagt tttatcaggc ttgaaatggc
     1321 gttccgcccc gactgtcgaa tcgtcggggc tgattttttc ttattattgc agcagaaact
     1381 tctcgaggaa ctggcgggtg cgaggctgct cgggatcggc aaataacgct tttgcggccc
     1441 cctgctcgac tatccgcccc tggtccataa aaatcgcccg gtccgcaaca tcccgggcaa
     1501 agctcatttc gtgtgtcaca atcaccatcg tgcgcttttc ctgcgccagc tgacggatgg
     1561 tgttcaggac ttcgcccacc agctctggat ccagcgctga cgttggctcg tcaaacaaaa
     1621 tcacctcagg acgcattgcc agcgcacgcg caatcgcaac acgctgctgt tgaccgccag
     1681 acaaacgacg tggatagctg gtttctttac cggccagccc aactttcgcc aacagcttgc
     1741 gagcgcgcgc cgtggcctct tctttcggtt cgcctttgac gatcaccggc ccttcaataa
     1801 tgttctccag caccgtacga tgcggaaaca aattaaagtt ctggaagaca aacccgacgt
     1861 gctgacgcaa ctggcgaatc agcgattttt gctgacttaa tgaacgtgca gtatcaatag
     1921 tgatatcgcc aacggtgatc gttcccgctt ccggttgttc cagcagattt atgctacgta
     1981 gcaatgtggt tttgccggaa ccactcggac cgataattgc caccacttcg ccaggcttta
     2041 cctcgaggtc gataccgtgc agcaccgtct gaccgtggaa ttttttcact aggttcttaa
     2101 cttcaatggc actcatttgg gttctctctc ctggcgatta agttgattct caaaatggtt
     2161 ctgcaacgtc gataacacag tcgccatgat ccagtagatg agcgaagccg ccagatacat
     2221 ggtgaatacc tccagcgtac gcgaggtaat caactgcgcc tgacggaaca gctccggcac
     2281 ctggattgtc gcggccagcg acgtatcttt taccagactg ataaagctgt tcgagagcgg
     2341 cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga cgcatagtct gccatggcgt
     2401 cataccaata ctggcagctg cttcccactg acctttatca atagaagaaa tggcggcgcg
     2461 aagcgtttcg gcagtatagg cggcagtgtt gagcgacaga ccaatcattg ctgacggaat
     2521 gggatctaat tcgataccaa actgcggcag gccgtaatag atcataaaca gttgtgcgat
     2581 gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc agccagcgca cgggccagat
     2641 tggcgacagg cgcatcagcg cgagaataaa ccctagcagt aaaccaaaaa acatgccgcc
     2701 gatactgagt tgcagcgtat acccagcccc tttcagcagg aacggcaagg aatcaataac
     2761 cagttgtata ctttcttgca tgagcgttcc cggagctaaa cgtggggatg ataggcgaac
     2821 agcgcaggcg cgccgccggt atgaataaac agaatcggcc cttcatcttt gaagcgtttc
     2881 tgactgatac cgtcaatcag acccgccatc gcttttccgg tatacacagg atcaagcaga
     2941 atgccttcca gccgcgccag caatttcact gcttccatgc cttcgtcgtt cggcacgccg
     3001 tagccaggtg caaaatagtc atcccagagt aaaatttcca ctgatgcggt cagctccagt
     3061 tctttcgcaa tcgcctgttg taggttaacc actttcggca attgatcggc aacggaacgc
     3121 gacacggtca cgccaatcag ttcgctttca ggcatcaggt gttccagccc aacagccagt
     3181 ccggcgtgag ttccggcact gcccgatgcg actaccaccg atgaaatatt aaccgcccct
     3241 tcacactgtt gcgcgatttc cagcgcactc tccacataac ctagcgcgcc aagagcatta
     3301 gaaccgccaa ccggaatgac atacgggcga aagccttgtg cttcgactcg cgtcgccagc
     3361 tcttccagtt gggcattggg atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac
     3421 agatccagca acaagcgatt gccgttggtt aaatagtttt ctgcggttgt gccaatagga
     3481 ttttccagca gcgccacgca gtgcagaccg agtttggcag cgactgcggc agtctgccgc
     3541 acatggttag actggatcgc cccggcagta atcagcgtat cggcaccttc acgcagagca
     3601 tctgccgcga gaaattctag cttacgtaat ttattgccgc ccattgccat gggggtgacg
     3661 tcatcccgtt tgatgaaaat ttcccgtcct agataatcag aaaagcgcgg cagatattcg
     3721 agcggcgttg gcgcgccgat aaactccagc cgtggaaaac gggttaaatt atgcagtggc
     3781 ataacagcct ccgatgtgtg ttgttgtgat tttctcatta tgcacgctga aaacgcgtaa
     3841 ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta tgctgattat ttggtcacat
     3901 cagcaccaaa ccatttttcg gaaagggctt gcaaagtgcc gtctttttgc atttccgcaa
     3961 ttgcatcatt cactgctttc agcaggtctt catttccttt acgcagcgcc acgccagact
     4021 cctgacggga gaatgcttca ccggttactg ccagcgtatc gttggttttc ttcaccagat
     4081 ccagcgccgc cagacgatca acgaggatcg catcgatacg ccctacgcgc agatcctgat
     4141 atttggtcgg gtcatcatca taggtacgca catcgacgcc ctgaacattc tgccgcagcc
     4201 actcttcata gttggtgccc agaccgaccc ccactttttt gcctttcaga tcatcggctg
     4261 ttttaatggt gccttcgtta ccttttttca ccagcgcctg aataccagaa atggtgtacg
     4321 gggttgagaa atcgtatttt ttcttgcgct catcagaaat ggtgacctga ttaatcacca
     4381 catcaatacg tttagagtcc agcgacgcca gcataccgtc ccatttggtc ggttttagtg
     4441 acgcctcaac gccaagatgt tttgccagct gttgggcaaa ttccacttca aaaccggtta
     4501 atttgccgtc atccccctga aaactgaacg gcggataagt accttccagc cctaccagca
     4561 gcgtgccgcg ctctttaact ttattaagca gaccttcatc tgcaaaactt ttaacgctca
     4621 tgcccgcaat cagcgctacg gccatcacac ccatcaatgc ctgacgtccc agatgtgcta
     4681 atttcatatt caccccgaat gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa
     4741 acacaaaaac aactccgcta catcttattc ttatttaata tatatcagaa gcaggcaggc
     4801 tggaggattt ctgcacaagt ccggtacagg tttggcgctg atagtgttga tacttccgta
     4861 acgcgatgcg gtaattgttg gtgctggtgg tgggcagcca cggggcgagg atttcatcaa
     4921 ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc gtgtagatga ttccccaaac
     4981 ggcgcagacg aacgacatat tcacgcaccg taccagggct catttcagtt tgctcaaaaa
     5041 gaaattgctt aaagccgata atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac
     5101 cgcaaaatcg gcacaatgcc gcccatgatt tttgttccgt ttgccagcca ttttcatcaa
     5161 gcagcgtgtc caggcgggaa atctcgattt tattcactat tttgccgtcg cgaactaagg
     5221 taatgcgatc gagcaattta cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt
     5281 taagatagcg gcttaatggc cgtcttttca ggtgctgcac catcattaag aactcctggt
     5341 agtcaaagtt aaagtgcggc atttactgac gttataactt acccagttta gtgcgtaacc
     5401 gtttaatagc ctggctgtgt aactgactga cccgcgattc cccgacctcc agcactgcac
     5461 caatctcttt gagattcagc tcttcctggt aatagagggt taataccagt ttttcgcgct
     5521 ccggcaacgt ttcgatagct tccatcaccc gctggcgcag attactgtcc agtagttgtt
     5581 gtagcgggtt ttctcgttga tgatcatcag taaccaattc gatgctatcg ccgtgctctt
     5641 cgcgccactc atcgtaggag aagagctggc tgttattggt gtcgagcaac atttggcgat
     5701 aatcggcaat atcgatccct aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa
     5761 gttcctgctc cagttgccct attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc
     5821 gcggcaccca gtcacggctg cgaagttcat ccagcatagc gccacggata cgctgcactg
     5881 cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg ttcgacggca ttaagtaacc
     5941 caatgccgcc cgcctgtagc agatcgtcaa gttccacgct cgcgggcagt cgaacctgca
     6001 ggcgcaatgc ttcgtgacgc accagcggga cataacgctg ccacagcgag tgtttatcca
     6061 ttacaccttc agcggtatag agtgaattca cgataaacag ccctgcgtta tatgagttat
     6121 cggcatgatt atccgtttct gcagggtttt taatcggacg attagtgggt gaaatgaggg
     6181 gttatttggg ggttacaggt aaattccagg cagaaaaaac cccgccggtg gcggggaagc
     6241 acgttgctga caaattgcgc tttatgttgc cggatgcggc gtaaacgcct tatccggcct
     6301 acaagaatgt gcaaattcaa taaattgcaa ttcaacttgt aggcctgata agcgcagcgc
     6361 atcaggcaat ttggcgttgc cgtcagtctc agttaatcag gttacaacga ttaaccctgc
     6421 agcagagaca gaacctgctg cggtacctgg ttagcttttg ccagcacgga gttaccggcc
     6481 tgctgaatga tctgcgcttt cgacatgttg gacacttcgg tcgcatagtc ggcgtcctga
     6541 atacgggact gcgcttcgga caggttggta gtggtgttgt tcaggttggt gactgcggaa
     6601 tccagacggt tctggatagc acccagggaa gaacggaatt tgtcgatgga gctgattgcg
     6661 tcgtccaggg cagcaagcgg gttggtcgtt gcagaaccag cactggtagt ctcagtagtg
     6721 attttacccg cggagttcac atttacagca gtaccaattg ctggagcata atctttattg
     6781 gtatcagtcg ctgaagcata cccggcaaca gtcacagagc cgttatcctt gttaacgctg
     6841 taagagactg tatagtcggc aacgttagtg ataccacctt tatcatccac ataagacttc
     6901 gcagcatcac tggattcacc cgcggtaaac ccaatagttt tgctcagtac accggaatta
     6961 aaggtaattg aagatgtcgc ggcagccgct ttggtttcgc tcaatactac atctttactc
     7021 actgtagcct gataagtaat cccaccaggg attaacggag ctactggagt ggttgtcgca
     7081 ggagttgctt ttgcgatggt atattctgtg ccattgaatg tcagagaggc accgtcacta
     7141 ccttcgctcg ctgctttgag cagcgctttc atatcagctt tagctgcgct accagcgttg
     7201 ttagtcgtca agttgcctgc atcgtctacg tatgcctggc ttccaccgat ggtgatatta
     7261 cctgctgaat ccgtttcgaa agaaacagta ccatcttttg tggtgtaaga accattaaca
     7321 gatttaccca catcagcatg aagggtggca gctaacgcat cacgttttga agctgaatca
     7381 gcagcctgcg ccgcggcgtc tacacccgta ccagcagtag atttagtggt agtaaaatca
     7441 ccagatttag cgttgtaagt ataatctacg ccgccaactg tgactttatc gccattccct
     7501 aatttatcga atgcagcatc ggtagttaac aaggtatttt cggttttcag atcataaaga
     7561 cctgtcgtgg tgtttaactt cgcgccagca gaagttaaat cacttaccgt tgcagctttg
     7621 ttggtaatag tacctttacc atttacgtta aagccattca gacccagagt atcagaatcg
     7681 atttttttca ggtcgatcgt gatggtttcg ccgtcattcg caccaacctg aattttcatt
     7741 gaaccgtctt tcgccagcac gttcacgccg ttgaactggg tctggccgga tacgcggtca
     7801 atttcatcaa gacgagattt gatttcgtcc tggatggagt ccaggtcaga atcggagtta
     7861 gtccctgtag tggcctgaac cgtcagttca cgaatacgct gtaagttgtt gttgatttcg
     7921 gacagcgcgc cttcggtggt ctgcgcaaca gaaataccgt cgttggcgtt acgggccgcc
     7981 tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc tgcggcgtca
     8041 tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact cgacagcgca
     8101 gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt attaatgact
     8161 tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgtattggg tttccacccg
     8221 tcggctcaat cgccgtcaaa cctgttatcg tctgtcgtaa aacaaccttt agaatttttt
     8281 tcacaaacag ccattttttg ttagtcgccg aaatactctt ttctctgccc cttattcccg
     8341 ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa ccccggtatt
     8401 cgttttacgt gtcgaatgat aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc
     8461 gggtcaggtc tggatttaag ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg
     8521 ctaaccccca tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg
     8581 ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa agccgatctt
     8641 ttttccgcca ccagcagcac caccgcgttc agtgccacca ccgcgggtaa cgccatcgcc
     8701 gggaaataca ccatcagcgt cacccatctg gcgcaggcgc aaaccctgac cacgcgcacc
     8761 accagagacg atacgaaaac ggcgatcgcc accagcgaca gtaaactcac cattcaacaa
     8821 ggcggcgaca aagatccgat taccattgat atcagcgcgg ctaactcgtc gttaagcggg
     8881 atccgtgatg ccatcaacaa cgcaaaagca ggtgttagcg cgagcattat taacgtgggt
     8941 aacggtgatt atcgtctgtc agtcacatca aatgacaccg gccttgacaa tgcgatgacg
     9001 ctctcggtta gcggtgatga tgcgctacaa agttttatgg gctatgacgc cagtgccagc
     9061 agcaacggca tggaggtctc ggtcgccgcc cagaatgcgc agctgacggt caacaacgtc
     9121 gccattgaga acagcagcaa caccatcagc gacgcgctgg aaaacatcac cctgaacctg
     9181 aacgatgtca ccacgggcaa ccagacgcta accatcactc aggacacctc caaagcgcaa
     9241 acggcgatta aagattgggt gaatgcctat aactcgctaa tagatacctt cagcagcctg
     9301 accaaataca ccgccgtaga tgcgggagct gatagccaga gttctagcaa tggcgcactg
     9361 ctcggcgact ccacgctgcg gacgattcag acgcagttga agtcgatgct gagtaatacc
     9421 gtcagttctt ccagctataa aacgttggcg cagattggta tcacgaccga tcccagcgat
     9481 ggcaaactgg aactggatgc cgacaaactc accgctgcac tgaaaaaaga tgccagcggc
     9541 gtaggtgcat tgattgttgg cgatggtaaa aaaaccggca tcacgaccac catcggcagc
     9601 aacctgacca gttggctttc gacaacgggc attattaaag ccgctaccga tggcgttagt
     9661 aagaccctga ataaattaac taaagactac aacgccgcca gcgatcgcat tgacgcgcag
     9721 gtcgcgcgct acaaagaaca atttacccaa ctggacgttt taatgacctc gttaaacagc
     9781 acaagcagct acttaacgca gcagttcgaa aacaacagta attccaagta atcattattc
     9841 atcgggagac aggtcatgta cgcggcaaaa ggcacccaag cctatgcaca aattggcgtc
     9901 gaaagcgccg taatgagcgc cagccagcag cagctggtca ccatgctatt tgatggagtg
     9961 ctgagcgcac tggttagagc gcgcctgttt atgcaggaca acaatcagca aggcaaaggc
    10021 gtctctttgt caaaagcgat caacatcatt gagaacggac tgcgggtgag tcttgatgaa
    10081 gagagcaaag acgaactaac ccaaaacttg attgctcttt atagctatat ggtcaggcgt
    10141 ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg aagaagtgga aacattaatg
    10201 cgcaatattg ccgatgcctg gaaagagtcg ttactctccc cttctttgat tcaggaccca
    10261 gtctgatgaa ccatgcaccg catttatatt tcgcctggca acaactcgtc gaaaaaagcc
    10321 agctcatgtt acgcctggca acggaagaac aatgggacga actcatcgcc agcgaaatgg
    10381 cgtatgttaa tgcggtgcag gagattgcac atttgactga agaggttgac ccgtccacca
    10441 cgatgcagga gcagctccgc ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa
    10501 agcagttatt acagattcgg atggatgaac tggcgaaact ggtcggtcag tcatcggtgc
    10561 aaaaatcggt gttgagtgcc tatggcgatc agggcggctt tgtgctggct ccgcaggata
    10621 acctcttttg aatctgaatg agtcgatggc tcgcgaataa tccgattacg gctacgcttc
    10681 taatgttccc cttgaatgga gtcgaagaat gcgtaatccc acgctgttac aatgttttca
    10741 ctggtattac ccggaaggcg gtaagctctg gcctgaattg gccgagcgcg ccgacggttt
    10801 taatgatatt ggtatcaata tggtctggtt gccgcccgcc tataaaggcg catcgggcgg
    10861 gtattcggtc ggctacgact cctatgattt atttgattta ggtgagtttg atcagaaagg
    10921 cagcatccct actaaatatg gcgataaagt acaactgctg gccgccattg acgccctgaa
    10981 acgtaatgac attgcggtgc tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga
    11041 aaaagaagct attcgcgtgc agcgcgttaa tgctgatgac cgtacgcaaa ttgatgaaga
    11101 aatcattgaa tgcgaaggct ggacgcgtta caccttcccc gctcgtgccg ggcaatactc
    11161 acagtttatc tgggatttca aatgttttag cggtatcgac catatcgaaa accccaatga
    11221 agatggcatt tttaaaattg ttaacgacta caccggcgaa ggctggaacg atcaggttga
    11281 tgatgaactc ggtaatttcg attatctgat gggcgagaat atcgattttc gcaatcatgc
    11341 cgtgacggaa gagattaaat actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg
    11401 ttttcgtctt gatgcggtca aacatattcc agcctggttt tataaagagt ggatcgaaca
    11461 cgtacaggaa gttgcgccaa agccgctgtt tattgtggcg gagtactggt cgcatgaagt
    11521 tgataagctg caaacgtata ttgatcaggt ggaaggcaaa accatgctgt ttgatgcgcc
    11581 gctgcagatg aaattccatg aagcatcgcg catgggacgc aactacgaca tgacgcagat
    11641 tttcactggg acgctggtgg aagccgatcc ttttcacgcc gtaaccctcg ttgccaacca
    11701 cgacacccaa ccgttgcaag ccctcgaagc accggtcgaa ccgtggttta aaccgctggc
    11761 gtatgctcta attttgttgc gggaaaatgg cgttccttcg gtgttctatc cggacctcta
    11821 cggtgcgcat tacgaagatg tcggtggtga cgggcaaacc tatccgatag atatgccaat
    11881 aatcgaacag cttgatgagt taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac
    11941 gttatttttc gaccatccga actgcattgc ctttagccgc agtggcaccg acgaatatcc
    12001 cggctgcgtg gtggtcatgt cgaacggaga tgatggcgaa aaaaccattc atttaggaga
    12061 gaattacggc aataaaacct ggcgtgattt tcttgggaac cggcaagaga gtgtagtaac
    12121 cgacgaaaac ggcgaagcga ccttcttttg caacggcggc agcgttagcg tgtgggttat
    12181 cgaagaggtg ctttaaattc atccccggcg gcaagccggg gagatttcat tacggcaatg
    12241 gcgtcggtaa cggcgcttta tccagcgcag cggcacactc tgccgtcggg cgatccacac
    12301 gctccatcgt cataccatcg tactcaatgg tgttaccgtc acgctcaacc tcgtacagct
    12361 cacgttttac ggtaacgtta gtgagatcat cagacatcaa tgtcaacttg cccggcacag
    12421 caatcacccg ctgccactga cggcaatcta gggtatcgcc ctctttagtg acaatcaggc
    12481 tgcctatcgc ttccgggctg accagcgcac gttgcggccc tttggtctgc cagtagcccg
    12541 ccagccagtc cggcgcgggg gttttcacga cattgttata gttttcgact tcggcacagc
    12601 ccgccagcaa catgagcgcg cctgcaattg cgagtttttt catcattcgt cctgcatgag
    12661 aagaaaagag gattgtggca ttaaagccct gatgtcgcca gcctttccag gttcagagag
    12721 aaatttgatc taactattat ttttgtgaat tattaccccg aaaataaatt tgcatggtgt
    12781 atgattcgcg actcttgttt caaaccttct gagttcagag gctacattca tgtcatggca
    12841 gcaattcaaa cacgcctggt tgattaaatt ctgggcgccc atccccgcgg tcatcgcggc
    12901 gggtattctc tccacttact attttggcat cactggcacc ttttgggctg tcacgggtga
    12961 atttacccgt tggggcggtc agctcctgca gctgttcggc gtccatgctg aagagtgggg
    13021 ttattttaaa attatccatc tggaaggatc gccattaacc cgcatcgacg ggatgatgat
    13081 cctcggtatg tttggcggct gctttgccgc agcgctgtgg gccaacaatg tcaaactacg
    13141 catgccgcgt agccgtatcc gcattatgca ggccattatt ggcggcatta tcgccggttt
    13201 tggcgcgcgt ctggcaatgg gctgtaacct ggcggcattc tttaccggta ttcctcagtt
    13261 ctcgctgcat gcctggttct ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg
    13321 ctttaccctg ctgcccatct tccgtattcc cgtgaaaatg cagaaagttg ctgccgcctc
    13381 accgctgacg caaaaaccgg atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt
    13441 tttcggcatg ctgggctggg cgctgctcac agcgatgaac caacccaaac tggggctggc
    13501 aatgctgttt ggcgtcggct ttggtttact gattgaacgt gcgcaaatct gctttacttc
    13561 ggcgttccgc gatatgtgga tcaccggacg tacccatatg gcgaaagcaa tcattattgg
    13621 tatggcggtg agtgccatcg ggatcttcag ttacgtacag ttaggcgttg aacccaaaat
    13681 catgtgggcg ggaccaaacg cggtaattgg tggtttactg tttggttttg gcatcgtgct
    13741 ggctggcggc tgcgaaaccg gctggatgta ccgcgcggta gaaggccagg tgcactactg
    13801 gtgggtcggt ctgggcaatg tgatcggctc aacgattctg gcgtattact gggatgattt
    13861 cgctccggcg ctggccaccg actgggacaa aatcaacttg ctgaaaacct ttggtccgat
    13921 ggggggcctg ctggtgacat atttgctgtt gtttgctgcg ctgatgttga ttatcggctg
    13981 ggaaaaacgc ttcttccgcc gtgcggcacc gcagactgct aaggagatcg catgaaaaat
    14041 atcgttcctg attaccgcct tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca
    14101 acccttgagg cgatgccgca gttaaaaaaa ggggaaatcc tggaagtggt gagcgactgt
    14161 ccgcagtcga tcaataatat tccactggat gcgcgtaatc acggctatac ggtgctggat
    14221 attcagcaag acgggccgac cattcgttat ttaattcaga agtaatccct cattcccgct
    14281 gtgacccaca gcgggaacat cgttaccttt gtatcacata gattttaccc gccccctctc
    14341 ctattctttt cagtatgatc tcaggaggtt tctatgtgtg gacgctttgc ccaatcccaa
    14401 acgcgtgaag attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctatgat
    14461 cccgaaccca ttggcagata caacgtcgcg ccgggaacca aagtcctact gctcagtgaa
    14521 cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg atggtgggat
    14581 aaaccgccgc tgattaacgc ccgcgtagaa actgcggcaa ccagtcgtat gtttaaaccg
    14641 ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa
    14701 ggcgacaaaa agcagcctta ttttatctat cgtgctgatg gacaacctgt ttttatggca
    14761 gcgataggca gcacaccgtt cgagcgcggt gacgaagccg aaggattttt gattgtcact
    14821 gcagcagcag accagggtct ggtagatatt catgaccgcc gcccgctggt tctgtcgcca
    14881 gaagctgcgc gggaatggat gcggcaagag attagcggta aagaagcctc agaaatcgcg
    14941 gccagtggct gtgttccagc aaaccagttc tcctggcacc cggtatcgcg cgcggtgggt
    15001 aatgttaaaa accagggtgc ggagttaatt caacctgttt gataagcctg gagattattg
    15061 atcaacaata ctgcgtcata agtaatctct attagacaaa gatttcatta cctgttggca
    15121 tattgcaaaa ataacaccaa tacggaatcg tcatgttcac gattaaaaca gatgatctca
    15181 cccatccagc agtgcaagca ttagtggctt accatatttc cggcatgctg cagcagtctc
    15241 cccctgaaag cagtcatgct ttagacgtgc aaaaattacg taacccgaca gtgacattct
    15301 agtcagtatg ggaaggcgaa caactcgcag gaattggtgc gctgaagttg ctggatgata
    15361 aacatggcga actgaaatca atgcggaccg cgccaaatta tttacgtcgg ggtgtcgcca
    15421 gtctgatttt acgccacatt ttgcaggtcg cccatgacag atgccttcat cgcctgagtt
    15481 tagaaacggg tacacaggct ggatttacgg cctgccatca actttatttg aagcatggtt
    15541 tcgttgattg cgaaccgttt gccgattatc aacttgatcc acacagtcga tttttgtcat
    15601 tgacgctatg cgaagataat gagttgcttt gagccagacg cagcacattc ttgcattcga
    15661 cgtgctgcgt ctttatttat caccaacagg aaacgccttg t
//
LOCUS       DAZGXS010000105        15572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGXS010000105 DAZGXS010000000
VERSION     DAZGXS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            81..329
                     /locus_tag="ACHKG5_003491"
     CDS             81..329
                     /locus_tag="ACHKG5_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0869526.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKG5_003492"
     CDS             307..1437
                     /locus_tag="ACHKG5_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0869527.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKG5_003493"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKG5_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0869528.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKG5_003494"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKG5_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKG5_003495"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKG5_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0869529.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKG5_003496"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKG5_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0869530.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKG5_003497"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKG5_003497"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0869531.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKG5_003498"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKG5_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0869532.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKG5_003499"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKG5_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0869533.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5873..7126
                     /gene="kch"
                     /locus_tag="ACHKG5_003500"
     CDS             5873..7126
                     /gene="kch"
                     /locus_tag="ACHKG5_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0869534.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHKG5_003501"
     CDS             complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHKG5_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0869535.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7497..8957
                     /gene="cls"
                     /locus_tag="ACHKG5_003502"
     CDS             7497..8957
                     /gene="cls"
                     /locus_tag="ACHKG5_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0869536.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8992..9321
                     /gene="yciU"
                     /locus_tag="ACHKG5_003503"
     CDS             8992..9321
                     /gene="yciU"
                     /locus_tag="ACHKG5_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0869537.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHKG5_003504"
     CDS             complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHKG5_003504"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0869538.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHKG5_003505"
     CDS             complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHKG5_003505"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0869539.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHKG5_003506"
     CDS             complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHKG5_003506"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0869540.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHKG5_003507"
     CDS             complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHKG5_003507"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0869541.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHKG5_003508"
     CDS             complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHKG5_003508"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0869542.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3978 a         3790 c         3679 g         4125 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata cctgccccaa cataaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat
     2281 caatttttaa atattttcgc tacgcaaata ttaactcgtt cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgccaaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccatattact ggccctggca ccggtgatgc tggtacagga caaaatgggc gtttttgcct
     3001 cgatccgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caaaatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctgaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtcctttgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg caaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagac gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ctgtacttga tgtcgggcgt gccggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt ttttgctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 cggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gagtagagat
     5581 ccaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttataggtc
     5641 ggtcattgca ttcaccacca caagcagata ataactttat ctgtatttct ttatatgatg
     5701 tgagcatgtt caggaaaaat gccctttcct gacgcagagt tgctcactca aaacctttga
     5761 ttactgcgga taaagtcatc tactattgtt taacacattc acgtgtcgca tgtatgttca
     5821 gaaaatgtgc acgttaagat aattaaacaa aattgtggtt acagaggtat cagtgagtca
     5881 ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc acgacattct
     5941 cgcgctggcc gtctttttaa atgggttgct tatttttaaa acaatctatg gtatgtcggt
     6001 caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct ccttgctggc
     6061 aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg gcttactgtt
     6121 ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc taatttacac
     6181 cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta cgctggtgtt
     6241 tttactgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga caatttttgc
     6301 atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt atttaagcga
     6361 aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga tagaaaccat
     6421 gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat tgttcactat
     6481 ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa tttttggtcc
     6541 gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa tgcatcgtaa
     6601 agatcatttt attgtttgcg gacattcgat tctcgccatt aatacgatcc tgcaactgaa
     6661 tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata tcaagcaact
     6721 tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg acagttctgt
     6781 attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg ataacgatgc
     6841 agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca aaacagttct
     6901 cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg atatcattct
     6961 ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa
     7021 taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat ggtattttca gcgataacga
     7081 tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa aaatagagtt taaagacgga
     7141 agagtaaact tattgggcac cgcaatggtg cccagtacat ttagatattg tagatcgcaa
     7201 acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg atacgcatat
     7261 ttcggcgcga ctgcccctca agccaacgcg atttacgtcg gctagcctga cgctgcatgc
     7321 gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc aataacagaa
     7381 tggtcattat acccccgccc ccgacgctga ccagtgcttt tcccgcgttt ttatttgcca
     7441 gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc taatctatga
     7501 caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg ctcattgcag
     7561 gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg gcctggctgt
     7621 tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt ggcgagctcc
     7681 atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc gcaaaatggc
     7741 ttaacgacct taaagcctgt aagcatatct ttgccgaaga aaatagcagt gtcgctgcgc
     7801 cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg aatcagctac
     7861 aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc cagctcgcgc
     7921 gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg gaccaggtgg
     7981 ctgaatcatt aatggcggct gcgcgacgcg gcattcattg ccgattgatg ctcgactccg
     8041 ccgggagtgt agcttttttc cacagcccgt ggcccgagct aatgcgtaat gccggtattg
     8101 aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt atggacctgc
     8161 gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc agcatgaata
     8221 tggtcgaccc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt gatctgatgg
     8281 cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc gactgggaga
     8341 ttgaaacagg gaaacgtatt ctaccgccac cacctgatgt caatattatg ccgtttgagc
     8401 aggccagcgg tcataccatt cacacaattg cttctggccc cggctttccg gaagatctca
     8461 ttcaccaggc attattgact gcggcttatt cggcgcgtga atatttgatc atgaccacgc
     8521 cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg cagcgcgggg
     8581 tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc tgggccagtc
     8641 gcgcattctt tacggaactg cttgctgctg gggttaaagt ttatcagttt gaaggcgggt
     8701 tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc accgttaacc
     8761 ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac gataaaggtt
     8821 ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt ctgctcgatg
     8881 cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg ttttacttct
     8941 tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac tatggatatg
     9001 gatctaaaca atcgcctgac tgaagatgaa acgcttgagc aggcctacga tatttttctc
     9061 gaactggctg ccgacaatct cgatccagct gatgttctgc tgttcaatct tcagtttgaa
     9121 gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca tgttgatttc
     9181 gatttgaacc cagacttttt tgccgaagtt gtgattggtc tggcggacag tgaagatggt
     9241 gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg aaaaagatca caaactttgc
     9301 catatcatct ggcgggaata aataatcact ttcggcgtga aaaagggctg acagctgtca
     9361 gccctttttg tgtttaaagc ggatcgactt tcaggcaaga aacggcgtgt ctgaaactcc
     9421 cctccagcac aggacgtgtt ttggcacact ccggaccggc aatcgggcaa cgggtacgga
     9481 aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt aactggatgg
     9541 ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat gccctggtgt
     9601 aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctacc gcatggccga
     9661 gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca tgagcgataa
     9721 aaattaatga caatcccatc tcacgttgca attgctggag caggttgacc acctgtgcct
     9781 gaattgacac gtccagcgcc gacaccggct catcacagat aatcagcttc ggttcaagaa
     9841 taagagcacg cgcaatcccg atacgctggc actgcccacc agagaactca tgcggatagc
     9901 ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg cgctcgcgaa
     9961 cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg atctcgccga
    10021 tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga atatcactgc
    10081 gaacggcacg ccattcatcg ggtttcatgc ccagcaactc tttacctaac caggcaacat
    10141 gaccgtcggt cgctttaacc aggccaatga tggcgcgagc aaaggtggac ttaccgcatc
    10201 ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagagtt acaccatcta
    10261 cggctttgag cgttttcggc ggttgccaga accactgttt gccatctttg atttcaaagt
    10321 gcactttcag atcggcgatt tcgaggagga cttttcttcc ttcggttaca gcattcataa
    10381 cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact cttccagcgg
    10441 cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt ggctggaatg ggcaaccttt
    10501 cggtaatcgc agcaggtttg gcggattacc cggaatagtc aacattgttt caccttccgc
    10561 atcgagacga ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg gttgataaaa
    10621 gacatcgcgc gcgttgccat attccatcgt gcgcccggcg tacattacca gcactttgtc
    10681 gcagatcccc gccaccacgc caagatcgtg ggtgatcata atgatggcgg tattaaattc
    10741 ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt acggtgacgt ccagcgcagt
    10801 agtgggttca tccgcaatca gcagcttagg tcgacatagc aatgccatcg caatcataac
    10861 tcgctgacgc atgccgccag aaaattcgtg cgggtacatt ttcatgcgtt tacgtgcttc
    10921 cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag ctttgctcat
    10981 gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat agggattcaa
    11041 cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt tattgagttc
    11101 acgctctggc aaattgagga tttcacgccc attgaaggtt gccgatccgc caatgcgtcc
    11161 gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg aaccagactc
    11221 acctacaatg cccagcgttt ctccggcacg tagggaaaaa tttaaatcat tgaccgccgt
    11281 gacgtcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca gcagtgcgtc
    11341 agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg ctgcactcct
    11401 taacgatctt tcgggtcgag ggcatcacgc aagccatcgc cgataaagtt gaaacaaaac
    11461 agcgtcacca cgaggaatcc cgctgggaac aacagtaacc atggagagac ttccattgag
    11521 ttcgcgccat cactcagcaa tgccccccag ctgcttaacg gctcttgcgt acccaacccc
    11581 aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagtagcga tgcgtagacc
    11641 accaccacac cgagtacgtt cggcacaatg tggcgaataa caatgcccga cgtcgataca
    11701 ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt ttgcccacgc
    11761 acaatacgag ccatatccag ccaggaaacc atgcctatcg ccacgaaaat cagcaggatg
    11821 ttttgaccga aaaaggtcac cagcaaaatg acgaagaaca tgaatgggaa ggagttgagg
    11881 atttccagca aacgcatcat taccgaatcc actttaccgc ccagataacc ggaaagcgaa
    11941 ccataaagtg tccccacgac cactgccacc agtgccgcag caacaccgac catgagtgag
    12001 atacgtccgc caatcgcaac gcgcacaagc aggtcgcgac cggatgagtc agtaccaaag
    12061 tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt atcgtcatag
    12121 gcaaactgcg aaagcatcgg tgccaggatt acaaataacg cgatcagcac cagcactatc
    12181 agacttgcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg ccacaagctg
    12241 cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt tttcttactt
    12301 aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc gcatatagca
    12361 catcgacaat ggcattaaac aaaatggtta acgcaccaac caggatggtc aggcttaaca
    12421 ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgcccaatc cccggcaaac
    12481 cataaatggt ttcgataacc atagaaccgg taataatgcc gacaaatgcg gggcccatat
    12541 aggagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc cgccgcatag
    12601 gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca atcatagagc
    12661 cacgggtaat acgcgcaata ctggcgatat aggcgagtga caacgccacc atcggcaata
    12721 tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc aaaatgatcg
    12781 caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc ccggtcattg
    12841 ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcggca ataacgccag
    12901 cactaacacc cagtattacc gcaaggaaaa atgctgcggc tcccaatttg gcagaaacgg
    12961 gaaaactgga tgcaaccagg tcattgaccg aataatcttt atatttaaac gatggaccga
    13021 aatcgccatg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc ggatcattaa
    13081 gatgatattt cgcttcgata ttggccatta cttccggcgg taaagtacgt tcgccggtaa
    13141 aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga ataaatagcg
    13201 tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta ccttctggcc
    13261 tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc ctgccccacg
    13321 tattgccatc agtgcttcac aatgtacata ttccgggtgt aggtattatc cagcggatct
    13381 ttgccggtat aaccaccaac ccacggtttc accagacgcg cattcacgta gtaataaaca
    13441 ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag agctgtgcgc
    13501 tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc cgggctctta
    13561 taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga agttggttcg
    13621 ttgtagtcag cacaccagcc tgcacgggcc acatcaaaag taccctggtg acgggtgtcg
    13681 aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt tttcttccac
    13741 aatgaagagg cagcaatcgc cagcttttta tgcagatcgg aggtgttata caacaggttg
    13801 atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt cgcttcttcg
    13861 ttacgttttt cctggctcca gccaaaccat tccggctgag tcaattttgc gccatcagta
    13921 tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt attaacaatg
    13981 atatcgcggt ccatacccag tttcagtgcg gtacgcacac gcacatcgtt gaatggcggt
    14041 ttctggttgt taatttcgta ataataagtg cacaggtatg gatcaacgtg aacttcgtcc
    14101 gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata agtcatgtcg
    14161 atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatggg caaataggtt
    14221 acctggttaa taacggtttt cgcgttgttc cagtaggttg ggctgcgttc aagaacgatt
    14281 cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat attaccaggc
    14341 tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga agttgatggg
    14401 tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc taatgtgtga
    14461 tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag aatttcatca
    14521 ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcggt gttcggatca
    14581 acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgcc atcagaccat
    14641 ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc ccaggattca
    14701 gcgacgccag gtgccggatg gccgtcaaga tcgctgacca gtaagccttc aaacaggtct
    14761 cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga ctgaacttct
    14821 gaaccattgt tacgtaccag cgtttgtttt tccgccagtg tgacgcctgc gggtacatca
    14881 gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc tactaaactt
    14941 ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact ggtattaacc
    15001 agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg ctgtcaggtt
    15061 actttatcgt tacggattgc tatcaccgac tttatttatt ctggtgattc agacgatcac
    15121 cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc gcgacgctct
    15181 ttaattgtta attattctca ttaccaataa agaatcgcct gacggttgcc cggaaagtac
    15241 caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcaatt ctcttttatt
    15301 gaccgtatca gaatctatag cttgcatcca tgtcttaaca aacatcttta aattcaatca
    15361 aatagagatt ggttaagcat gtcgatcttt tctgattaca gttccagtag tgaaatgcac
    15421 aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag tggaattact
    15481 aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga
    15541 tgatgaggag aagcagttaa ggaaatcctt at
//
LOCUS       DAZGXS010000106        15506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGXS010000106 DAZGXS010000000
VERSION     DAZGXS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(129..410)
                     /locus_tag="ACHKG5_003509"
     CDS             complement(129..410)
                     /locus_tag="ACHKG5_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFP0869543.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(672..1781)
                     /gene="apbC"
                     /locus_tag="ACHKG5_003510"
     CDS             complement(672..1781)
                     /gene="apbC"
                     /locus_tag="ACHKG5_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0869544.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1913..3946
                     /gene="metG"
                     /locus_tag="ACHKG5_003511"
     CDS             1913..3946
                     /gene="metG"
                     /locus_tag="ACHKG5_003511"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0869545.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4087..4815
                     /locus_tag="ACHKG5_003512"
     CDS             4087..4815
                     /locus_tag="ACHKG5_003512"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFP0869546.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6772..7896
                     /locus_tag="ACHKG5_003513"
                     /pseudo
     CDS             6772..7896
                     /locus_tag="ACHKG5_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7909..10894
                     /locus_tag="ACHKG5_003514"
                     /pseudo
     CDS             7909..10894
                     /locus_tag="ACHKG5_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10904..14533
                     /gene="yehI"
                     /locus_tag="ACHKG5_003515"
     CDS             10904..14533
                     /gene="yehI"
                     /locus_tag="ACHKG5_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP0869547.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14595..14912
                     /gene="yehK"
                     /locus_tag="ACHKG5_003516"
     CDS             14595..14912
                     /gene="yehK"
                     /locus_tag="ACHKG5_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP0869548.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4155 a         3848 c         3742 g         3761 t
ORIGIN      
        1 aacatttttg aaactgatta attaccgtaa attaccgtaa ataacattta tgggatgaat
       61 aaaaaagcca aaatcattca taggaatgat tttggctttt taaatatgct taaaacggtg
      121 ttggcaaatt aatcgtagcg atttaacacg attttgcgtt tgtcgttatt acccacagct
      181 accacttctg tcgtaacgcc ttttgcagta gaagaagcac gatagttggt ttcaactcgt
      241 tgtaacaact gcccacaacg ttgctgaaga tgattattaa caacatccat actgcatgta
      301 ggggaactaa ttctgccatt gatggttaat gttgtagtat cagaagagaa cgcgggtgat
      361 gctagtccat aagcaagaaa aacagctcct gacaaccaat acttattcat tgaacgttat
      421 ccctgtagta aaggttatgc cgggtagatt gagtgcataa cacactatag ctgtacatcc
      481 gctacacagc cacgagggat gataaagaga cagtggttgc acaatcaatc gcctttgtac
      541 agtttcggac taagatccac tgaatattcg cagtattaaa taagtgttca ttaatgcggc
      601 aaaaacaaat acaccgcatc aatataacat ctctgcggtg tatgaagaat aaccagaatg
      661 gttaataggc gttagaccgc gcggaaggaa atctcgcctg gaatgacttc accctgccag
      721 tagagctgag ctgcaacgcg gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc
      781 ggacggctga taaccgttgg cgtgccttta tcgagatcct cacgtaagga gatgtgtaac
      841 ggcatctgcc ccagcagctg tgtatggtat ttttcagcca gtttctctgc accaccggta
      901 ccgaaaatcg gttcatggtg accacagtta ctgcaaatat gcacactcat gttctcgaca
      961 ataccgagca ccggaacctc gactttctcg aacatcacaa tgcctttttt cgcatcgatc
     1021 aacgcgatgt cctgcggggt ggtgaccacg actgcaccag ttactggaat gttttgcgcc
     1081 agcgtcaatt gaatgtcacc ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc
     1141 ggccacaagg tttcctgcaa catctgcatt aacgccttgc tcgccatcgg tccacgccac
     1201 accatcgcat tgtcgtcagt gaccagataa ccaattgaat tggttgccag gccatgagac
     1261 ataatcggtg ccatatgggt accgtccggc gaagttggac gctgattttc tgcgcccagc
     1321 atggtgggaa ttgatgggcc gtagatatcg gcgtccagaa tgccgacttt cgccccttcc
     1381 gccgccagtg ccagcgccag gtttaccgcc gtggaggatt tacccacgcc gcctttaccg
     1441 gaactgaccg caatgatgtt cttcacgcca ttaatacccg gctgattctt cacgcgtttt
     1501 aaggtggcga tattgtgcga cagtttccag tcgatagctt tcgccccggt gatacgcagc
     1561 agttcggcgc tacattgctc ttttaactct tcaaacgcgc tatgccagac gaatggcata
     1621 accagttcca catgcaacgt gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg
     1681 gtgaggttat gcttcagggt tgggtgctga aaattggcca gtgtcccggc gaccattgcg
     1741 cgcaaggctt ctggtgattt ggcctgggat tgttcgttca tcccgactcc ttttttgtat
     1801 agataaacca tcagctgata gtttacctga agaatataga gaagtactta cttaacattt
     1861 tcccatttgg tactatctaa ccccttttca ctattaagaa gtaatgccta ctatgactca
     1921 agtcgcgaag aaaattctgg tgacgtgcgc actgccgtac gctaacggct caatccacct
     1981 cggccatatg ctggagcaca tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg
     2041 ccacgaggtc aacttcatct gcgccgacga tgcccacggt acaccgatca tgctgaaagc
     2101 tcagcagctt ggtatcaccc cggagcagat gattggcgaa atgagtcagg agcatcagac
     2161 tgatttcgca ggctttaaca tcagctatga caactatcac tcgacgcaca gcgaagagaa
     2221 ccgccagttg tctgagctta tctatactcg cttgaaagaa aacggtttta ttaaaaaccg
     2281 caccatctct cagctgtacg atccggaaaa aggcatgttc ctgccggacc gttttgtgaa
     2341 aggcacctgc ccgaaatgta aatccccgga tcaatacggc gataactgcg aagtctgcgg
     2401 cgcgacctac agcccgactg aactgatcga gccgaaatcg gtggtttctg gcgctacccc
     2461 ggtaatgcgt gattctgaac acttcttctt tgatctgccc tctttcagcg aaatgttgca
     2521 ggcatggacc cgcagcggtg cgttgcagga gcaggtggca aataaaatgc aggagtggtt
     2581 tgaatccggt ctgcaacagt gggatatctc ccgcgacgcg ccgtacttcg gttttgaaat
     2641 tccgaacgcg ccgggtaaat atttctacgt ctggctggat gcgccgattg gctatatggg
     2701 ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta agcttcgatg aatactggaa
     2761 gaaagactcc accgccgagc tgtaccactt catcggtaaa gatattgttt acttccacag
     2821 tctgttctgg ccagcaatgc tggaaggcag caacttccgc aagccgacca acctgtttgt
     2881 tcatggctat gtgacggtga acggcgcgaa gatgtctaag tctcgcggta cctttattaa
     2941 agccagcacc tggctgaatc attttgacgc cgacagcctg cgttactact acactgcgaa
     3001 actctcttcg cgcattgatg atatcgatct caacctggaa gatttcgttc agcgcgtgaa
     3061 tgccgatatc gttaacaaag tggttaacct ggcctctcgt aatgcgggct ttatcaacaa
     3121 gcgttttgac ggcgtgctgg caagcgaact ggctgacccg cagttgtaca aaaccttcac
     3181 tgatgccgct gaagtgattg gtgaagcgtg ggaaagccgc gaatttggta aagcagtgcg
     3241 cgaaatcatg gcgctggctg atctggctaa ccgctatgtc gatgaacagg ctccgtgggt
     3301 ggtggcaaaa caggaaggcc gcgatgctga cctgcaggcg atttgctcaa tgggcatcaa
     3361 cctgttccgc gtgctgatga cttacctgaa gccggtactg ccgaaactga ccgagcgcgc
     3421 agaagcattt ctcaatacag aactgacctg ggatggtatc cagcaaccgc tgctgggcca
     3481 taaagtgaat ccgttcaagg cgctgtataa ccgcatcgat atgaagcagg ttgaagcact
     3541 ggtggaagcg tctaaagaag aagtgaaagc agctgccgcg ccagttactg gcccactggc
     3601 agatgatccg attcaggaaa ccatcacctt tgacgacttc gccaaagttg acctgcgcgt
     3661 ggcgctgatt gaaaacgcag agtttgttga aggttctgac aaactgctac gcctgacgct
     3721 ggatctcggc ggtgaaaaac gcaatgtctt ctccggcatt cgttctgctt acccagatcc
     3781 gcaggcactg attggtcgtc acaccattat ggtggctaac ctggcaccac gtaaaatgcg
     3841 cttcggtatc tctgaaggca tggtgatggc tgccggtcct ggcgggaaag atattttcct
     3901 gctaagcccg gatgccggtg ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc
     3961 gctgcatcga cagcgccttt tctttataaa ttcctaaagt tgttttctta cgattttgtc
     4021 tctctctaac ccgtataaat actggtagca tctgcattca actggataaa attacaggga
     4081 tgcagaatga gacactttat ctatcaggac gaaaaatcac ataaattctg ggcggttgag
     4141 cagcagggaa acgagctgca tatcaactgg ggcaaagtcg gcactaacgg gcaaagccag
     4201 ataaaaagtt ttgcggatgt tgcagcagcg gaaaaagcga aactgaagct tatcgcggag
     4261 aaaacgaaaa aaggctatgt ggaaaatgct tcagcaaacg tgcatatccc ccccatcacc
     4321 aaagccactc ctgaagttga aacttcccct gagagtaaaa accaacgccc gtggctggct
     4381 gatgatgcag ttattccggt aactgacgat attaaccgat ttgcttttcc tcatcgctct
     4441 cgccccagag agatcaatta tctgcgcaaa gacggtgaga tatggaagcg tattgcagat
     4501 aacaccaggg catatgatcc cgacaacaat taccgctctt atcctgaaaa ctggcagcag
     4561 gcttttgctg agttacaaag gcgaattctg gataatcaac agacagggag tgcgcaatct
     4621 gatgcagcat tgctctggag tttttggaac tcttactctg cagatgaact ggtcgatgac
     4681 ttagtcattc gctgtggtct ggaaagcgcc gttgaaatcg tcctttttgc ccttcaactc
     4741 aaatataaac cagtaaaagg cgcagtaacc accatcattc cacctaatca cgaagcagaa
     4801 tcgctaccta gttagcatca acgtctatgt catcatcttt cactcgcttc agaagatgag
     4861 tggcaacgct gtgtagataa agtactcacc gccattccga cgctatcccc cgcacggcaa
     4921 ccttttgcgg cgttactgct ccctgaacgc ccggatatcg cccatgctat agatctacgc
     4981 tatgcagacc aaaacgtccc ggcgatgacc tggttaagac tggtgataac cgatgatact
     5041 gctctgagta cagtggaaaa atacgacttc ccaccgttgt atcgtgattt tcgtaatttt
     5101 cgcgcttatc tggcaatgtt gttggcaaat aatggggtgc gcggtgtaag ccggatactt
     5161 cttgagttta cggaagacca ttctgacaat cccacttatc tcttcgaacg catcagcgag
     5221 actgaaaatc ttgtcaaatg gctatggaaa acgaatcacc cggatgcgat tcaaattctg
     5281 atcctcggtg taattggtaa gaaaaagcac ctggaatact taagcaaggc cagtcaaaag
     5341 catcccgctg cggctattgc ggcttatgct actttgctgg caatacatga agataaagag
     5401 tggcgtaaag cgcttgtcaa actgattacc gccacgccag agttagtatg cgacgtcatt
     5461 ccctgggtta atgctaaagc ggcaggcatc ttatctgaat gtcgtccgca gtcagtcgct
     5521 gaagaatgtg aatatgccac tgtggatatg ttgccggagc tgcttgtttc gccgccctgg
     5581 atgaccaaag agaaaaagaa aaatacgcct gtgtttgatc ttccagtgct tccagttcct
     5641 tctgtttctg atgtcactcc agagattacc aaaaaactga cccgtactta ccttgtcacc
     5701 cacttccagc aaatagccca acagcaggcg acaaaacaaa cattgtttac cgacctgccg
     5761 ccgatcaaaa aagcaagctg ggagaaacac ttaataccgc ttacaccgga gcaacaaata
     5821 ctgtggcatc ttggttttga aaaatggcgg gagagcggag aaaaaatata cgaaaaaatt
     5881 ccggcgccac aaagcgccgt tgatgccctg ctacgcttcg attttccagc actcaatgct
     5941 gaatttgtgc attatcataa caatgcatac aaaagctgga atctcattgc cctctgttac
     6001 ctgcctggtc aacaggccat ttcttttctt aaccagatag tcaaagaaga taactactct
     6061 ggtgagggaa atattctggc aatttttggt agcgccgcca tacctgcatt tatggcctgc
     6121 ctgcaacgtg atccacgacg tctgtgcttc ttcccctttt tcctcggtgt cagtgaactg
     6181 gccttaccga tggcgcaaca attacagaaa aaaatgtctt atgaagatgc gcgaaactgg
     6241 ctaactgatt atccacgcca tgccgccgcc gggctgcttc ctgtggccct gggcaaaaaa
     6301 ggaaaagatc gtgattgtgc ccgccaggcg cttcgtctgc tggtgaattt aaaccagcga
     6361 gaaacgattg aggaaatcgc gcaaggatat aatcaacctg atgttttagc tgcgctggca
     6421 acattgttcg atagcgaccc actggaagaa tatcccgcta aaatcgcccc actgcccggc
     6481 ttttatcagt tcaccttgtg gcgcagacca cggcttaaaa gtaacaacct gcctctgtca
     6541 gatgacgcta tgcgccacct cggcactatg ctgagctttc ctcgcgacat taccgcctac
     6601 gctgggctgg atatcattag agaaatcttc acccgcgaat cactggctga atttggctgg
     6661 gatctgtatc ccgcctggac agaagctggc gcacccgcaa aagaaaactg ggcatttact
     6721 tcgcttggga ttttaggcaa tgacgacacc gcgcgtaaat taaccccgct tatccgcgcc
     6781 tggcctgggg aatcccagca taaacgggcg gtgtccgggc tggatgtatt agctgatatt
     6841 ggcagcgatg ttgcgctaat gctgcttaat ggcatcgcgc gaaaaattaa attcaaagca
     6901 ttacaggaac atgcccgcga gaaaatcaac atagttgctg aaaaccgtgg gctgactatg
     6961 gctgaactgg aagaccgcct ggctccagat ttagggcttg atagcagtgg ctcgctgata
     7021 ctggatttcg gcccccgcaa attcaccgtt ggttttgacg aaaccttaaa acctgtagtg
     7081 tgcgatgcaa acggcaaagt cctgaaagat ttacctaagc caaaccagag cgatgaaaaa
     7141 actcaggcaa ctgacgcggt taatctcttc aaacagttga aaaaagatgt acgcgccata
     7201 gccagccagc agattgatcg tctggaacag gctatgtgcc agcgcagacg ctggacggca
     7261 gagcagttcc gcctgtttct ggtggagcat ccgctggtac gtcacttaac ccgacggctg
     7321 ttatgggggg tttataacga tgaaaacgcc cttatcacct gttttcgcgt ggcagaagac
     7381 agcacttaca gcgatgcgca ggatgagtta ttcacgctgc cagcaggaaa catcggtatt
     7441 ccgcatgtgc tggaaatatc ccctgaatcc gccgctgcat tcaggcaaat ttacgctgat
     7501 tacgaactgc ttcccccttt ccaacagctc gagcgaggta gctatcacct tgctgataat
     7561 gaacgtaata ctcacgaact gacacgctgg caaggacggc tttgccaggc cggacgcatt
     7621 gtcgggctgg aacgcagagg ctggcaacgt ctggaagaaa gcggcagcgt ttatgcgatg
     7681 cgcaaaacaa cccctcatgg cgatctcgaa cttgaaacgg aacctttttc attaatttat
     7741 ggtgaaacgg ggtatggcga ccagcacccg gttgaaagcg taaaaatcac ctcgccagat
     7801 gatcgctacg gtaaacaatc ctcactcact ttctccatgc tcgacgacat caccgccagc
     7861 gagctgatta acgacattga atcactgttt gattaaaagg atttaacaat gaaaacctat
     7921 atttatcagg acgaaaaatc acataaattc tgggcggtgg agcagcaggg aaacgagctg
     7981 catattaact ggggtaaagt cggtaccaac gggcaaagtc aggtaaaaag ctttgcagat
     8041 actgcggcgg cggaaaaagc agcactgaag ctgattgcgg agaaggtgaa gaaggggtat
     8101 gtggagcaag tagacgctaa cgctcactca catgatgcct cagcaaagaa agtcctggtc
     8161 acagaggaaa aagcctcaat aaatcgccag gcagcaacta atggtttacc ctggctggca
     8221 gacaacgatc ccattattct tcccccgaat attgcccgcc atgccctcag ccaccgcctt
     8281 tggccgggag agccagtaaa aaaaccaacc aaacccgaga aacaaaattt aatttcccga
     8341 ttggcaaatc gtacttatca ttattataaa aatctgactt tcgattacag ctcatgctct
     8401 gatgaatggg ctgatgcgat tagccaggca atcagcttaa ttaaggccca aaagactgag
     8461 atacccgcgc gtactatggc ggtacttact tttctggaaa tatgtttttg tcagggctgg
     8521 aatgatgaaa cctcatccga gttgctggat gaaattgtaa gcaccttcgg tatcgaatac
     8581 gcaactgaaa ctgtcatctt ctggtggcaa attgatttcg attttgacta taaagatgaa
     8641 catttgactt tctttattaa ttatgcagat agcagcaagg acagttatag aaggaataac
     8701 gacaaattta gtttacgtct gcgtaaacat ttatcactag cagaagaaga agtatggcaa
     8761 aattgcattg caaaattact cgttgcgtta ccagatatat cactctatcg ccaaccgtta
     8821 attgccattt taatgccaga aatacccgaa gtggcccatg agatcgtaca tcgtttacac
     8881 caggtcgcag atgttccaca acttgaaatg ttgaaactgg tagcgacaga tccattcacg
     8941 ctcgaaatac tggagaacta tcaatacatt gatgtattta acacctatgg tgccagttgg
     9001 tcagctacgg tattacggga gcaaggtata gcggcactgg ctcgcctgac tccctatgct
     9061 gaagaagata attgcggtga cgtactgaaa tgtattaatc atccgttggc cattacgaat
     9121 aaaaagtgcc gaaaattttc cttctgcaac gatagtggca ttagccgaag cattggtaaa
     9181 aaaagatgat aaacgcaaac atacattgct ggtttcatta cttaatgcaa atcctttatt
     9241 tgtcgaaaaa ttagtcatcg gattatcgtc gcaagcggca aatttattgc aacaatgtca
     9301 gcaattgctg aatagtcgca atgaagattt tgcaaactca gaaattctac caatggtact
     9361 agtctcgccg ccctgggaaa ctcaaaataa aaataacgac aacagtcctg tgggtaaata
     9421 cccggcgaaa atcccccctt taccgttgtt ttatcaacct ccattatgga ctcgcccgaa
     9481 actcaaaaat agcagcaagg tactgccaga tagcgcactc cagcacctcg gtgaaatgct
     9541 ccgcttccct caggaagagg ccctgtatcc gggattattg caggtgaaag atgcctgtac
     9601 cgccgactca ctggctgaat ttgcctggga tctgtttacc gcctggctga ctgcgggcgc
     9661 accgtcgaaa gagagttggg cgttcactgc gttaggcgtt cttggtaacg atgacaccgc
     9721 ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata aacgcgccac
     9781 cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc agcttaacgg
     9841 tattgcccag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa aaattgccgg
     9901 cattgccgaa agccgcgaac tcacggtggc agagcttgaa gatcggttag caccggatct
     9961 cggtctggat gataacggtt cgttgctgct ggatttcggc tcacgccagt tcaccgtcag
    10021 ctttgatgaa accttaaaac cgtttgtgcg tgatgtttct ggcagccgtc tgaaagacct
    10081 gcccaaacca aacaaaagcg atgatgaatc acaggcgaac gatgctgtta accgctacaa
    10141 actgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggt tggaatccgc
    10201 catgtgcctg cgtcgccgct ggtcgccaga aaactttcag ctcttcctgg ttgaacatcc
    10261 actggtacgc cacttaaccc gccgtctgat ttggggcgtt tatagcaccg aaaaccagct
    10321 actgacgtgc ttccgcgtgg cggaagataa cagctacagc accgctgacg atgatctttt
    10381 caccctgccg gaaggcgata tctctgttgg cattcctcac gttctggaaa tatcaccgac
    10441 ggatgctacc gcctttggtc agctttttgc cgactatgaa ctgctaccac cgttccgtca
    10501 gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg aactgacccg
    10561 ctgggcaggc agaaaatgcc cgagcggtcg ggtcatgggg ctggcgaata aaggctggat
    10621 aaagggcacc ccgcaggatg gaggctggat cggctggatg atcaaacctc tgggccgctg
    10681 gtcgttaatc atggaaatcg atgaaggttt tgcggtaggc atgtcgccag ccgaactgag
    10741 tgccgagcaa atcttaagca agctgtggct atgggaaggc aacgcagaaa gctatggctg
    10801 ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca ccgccagcga
    10861 gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca aggaattacc
    10921 gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa cgccgctcag
    10981 tcatcgtcgc tggccgggcg aaccagtgtc cgttatcact ggaagtctca tccagacatt
    11041 gggagatgaa ttactacaac aagcaggcca gaaggaaaat atcacctgga attatgataa
    11101 atgctcactg gagtggcagt ccgccatcca gcaggcaatc aatttgactg gcgagcataa
    11161 accgtcaatc ccagccctta ccatggcggc tctgatctgt atcgcgcaaa atgacagcca
    11221 gcagttactt gatgaaatcg tgcaacaaga ggggctggaa tatgcgacgg atgtagtgat
    11281 atcgcgtcag tgcattgcgc ggcgttatga gagtgactca ctggtcgtca ctttgcagta
    11341 tcaggacgag gactatggct acggttatgg ctccgcgacc tataacgatt tcgatctccg
    11401 tttgcgtaag catctgtcgc tggcagagga aagttgctgg cagcgttgtg ccgataaact
    11461 cattgccgca ctgccaggaa tacccaaaat tcgccgtcct tttattgcgc tgatcctccc
    11521 ggaaaaacct gaaatagcca atgaactcgc cagtcttgaa agctcccggt caagtctcca
    11581 ttcaaaggag tggttaaagg tcgttgctac tgataatacg gcggtaaaaa aactcgaacg
    11641 atactggggc ctggacgtat ttagcgatcg cgaagccagc tacatgtcac aagaaaaccg
    11701 cttcggctac gcggcctgcg cctccctttt gcgcgaacaa ggactggcag ccgttccgcg
    11761 cctggcaatg tatgctcata aagaagattg cggcagtctg ctggtacaaa ttaaccatcc
    11821 gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc tgcaacgtgt
    11881 ggctaaatac agtaaaaact tcccccatgc gacgctcgcc gcactggcag aactgctggc
    11941 gttaaaagaa ccaccagccc gccctggtta tccaatcatc gaagacaaaa agctgcctgc
    12001 acagcaaaaa gctcgagaag aatactggcg tacgctgtta caaacgctga tggcatcgca
    12061 gccacaactg gcagcagaag tgatgtcgtg gttaagtact caagccaggg cagtgctgaa
    12121 gagttattta tcggcactgc caaaaccggt tattgatggc accgataaca gcaatctgcc
    12181 tgaaatcctc gtttcaccac cgtggcgtag taagaaaaaa atgacagctc cacgtcttga
    12241 tttggcacgg ctcgaattaa ctccgcaagt ttactggcaa ccaggcgaac gagagaggct
    12301 tgccgccact gagtctgccc gttatttcag cacggaatct cttgcgcaac gcatggaaca
    12361 aaaaagtggg cgagttgtat tacaggaact gggttttggg gatgatgtat ggctgttttt
    12421 gaattatata ctccctggaa aactggatgc tgcacgcaat tcactcattg ttcagtggca
    12481 ttactaccag gggcgggttg aagagatcct gaatggctgg aactccccgg aagcacaatt
    12541 agcagaacag gcgctccgca gcggtcacat agaagcgtta attaacatat gggaaaatga
    12601 caactactca cgttatcgtc cggaaaagag tgtctggaac ctgtatttat tggcacagtt
    12661 gccgcgtgag atggctttga ctttctggct gcgtatcaat gagaaaaagc atctgttcgc
    12721 gggagaggac tattttctca gtatcctcgg attggatgcg ctaccaggtc tgctgttggc
    12781 tttttcacat cgtccaaaag aaacatttcc gttaatttta aatttcggcg caacagaact
    12841 ggccctgccc gttgcccgcg tctggcgacg tttcgcggcg cagcgtgatc tggctcgcca
    12901 gtggatttta caatggccgg aacatacggc tactgcactt atccctcttg tcttcaccaa
    12961 atctagcgat aaaagcgaag ctgcattact tgccctgcgt ttactttacg aacatgggca
    13021 tggcgaattg ctacaaactg tagccaatcg ttggcagcgt acagatgtat ggcctgccct
    13081 ggagcattta ctgaaacagg gtcccatgga aatttacccg gcacgcattc caaaagcccc
    13141 tgatttctgg catccgcaaa tgtggtccag gccgcgcctt atcactaata atcaacctgt
    13201 taccgatgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg ggggacgttt
    13261 ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc
    13321 ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc gcaaaagaca actgggcatt
    13381 tctggcgtta agtctctttg gtgacgaaag cacggcacgg gatctaacaa cacagatcct
    13441 cgcctggcca caaggcaaat ctgcccgtgc tgtcagcggc ctgaacatcc ttaccctgat
    13501 gaataatgat atggcgctga tacagctgca tcatatatcg caacgggcta aatcccgccc
    13561 cttacgtgat aacgcggcgg aatttcttca ggtagtcgca gaaaatcgcg ggctaagcca
    13621 ggaagagcta gcggacagat tagtcccaac cctgggcctt gatgatccgc aggcgttgag
    13681 ttttgatttt ggtccccggc agtttaccgt tcgcttcgat gaaaacctca acccggttat
    13741 ctttgatcag caaaacgttc gccagaaaag cgttccccgg ttgcgcgccg atgacgatca
    13801 actgaaagcg cccgaggcac tggcccgact aaaagggcta aaaaaagatg ctactcaggt
    13861 gagcaaaaac ctgctcccgc gtcttgaagc tgccctacgt accacccgac gctggtcgct
    13921 ggcagatttt cattctctgt ttgttaatca tccctttacc cgtctggtta cccagcgatt
    13981 aatatggggg gtttatccgg caaatgaacc gcgtcgttta ctcaacgcct ttcgtgtggc
    14041 cgcagagggg gggttctgca atgcgcaaga tgagccaatt gacctgcctg cggacgctct
    14101 gattggcatt gcccacccgt tagaaatgac agcagaaatg cgcagtgaat ttgcacagct
    14161 ttttgccgat tacgaaatta tgccgccttt tcgccagttg gcgcgccgca cggtgctgct
    14221 cacacctgat gagtcaacca gtaacagcct gactcgctgg gaaggtaaat ccgctaccgt
    14281 tgggcaactt atgggaatgc gatacaaagg ctgggagtca ggttatgagg acgcatttgt
    14341 ctatgacctg ggtgagtacc ggctggtcct taagttttca cccggtttta accactacaa
    14401 tgttgatagc aaagcgttaa tgagcttccg ttctcttcga gtgtaccgtg acaataaatc
    14461 cgtcactttt gccgaacttg atgtgtttaa tttgagtgag gcgttaagcg cacctgacgt
    14521 cattttccat taaaaacaca gcgggcagtg atgcaactgc ccgttatcaa cacgacttac
    14581 cagcggggaa agcgatgatc gtgcaaaaag agctggttgc tatttacgat tatgaggtcc
    14641 ctgtacctga agatccgttt tccttcagac ttgagatcca taaatgctct gaattattta
    14701 caggttccgt ctatcgactg gagcgattcc ggctacgttc aacatttcat caacgtgatc
    14761 gagaagatgc tgacccgcta ataaatgatg cgttgattta tataagagat gagtgtattg
    14821 atgagcggaa attacgaggt gaatcacctg aaactgtaat agcaattttt aatcgtgaac
    14881 tacagaatat attcaaccaa gaaatagaat aatatactct agataattaa agtcgcagca
    14941 catgcaactt gaagtatgac gagtatagct ttcttaaagg gggaaacggt attccccctt
    15001 agatgcgttt caatttgccc gttacttcaa caaatcaatc aaccgtaaca gagccactaa
    15061 taactccagt acggcaatga gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc
    15121 ttattaagga gcgcaacacc agcaccttac ccttacactg cctgtcgata ctggtttacg
    15181 tatgttggta cgaaacgttg ctctccggat cagggcaccg cctgcggcac cacccgtccg
    15241 gcagccagga cttaaaattg gtccgatccg gcacaacctc ccaacgggca ggattataaa
    15301 tctcactcac tgaattgcaa tggagtgtgg atatggttat aatttattcg ttggtacgaa
    15361 atgttgcaca atcagcaagt tagcgtcact gtagtaaata aaagaacctg cctcaccagc
    15421 aggttttttt attttctgcg atctactttc cagatatttt tttgccaata gcccaccata
    15481 aatattcaca tcccggtcaa atggtg
//
LOCUS       DAZGXS010000107        15288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGXS010000107 DAZGXS010000000
VERSION     DAZGXS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(178..243)
                     /locus_tag="ACHKG5_003517"
                     /pseudo
     CDS             complement(178..243)
                     /locus_tag="ACHKG5_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(254..359)
                     /locus_tag="ACHKG5_003518"
                     /pseudo
     CDS             complement(254..359)
                     /locus_tag="ACHKG5_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            539..712
                     /locus_tag="ACHKG5_003519"
                     /pseudo
     CDS             539..712
                     /locus_tag="ACHKG5_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            823..1746
                     /gene="yicL"
                     /locus_tag="ACHKG5_003520"
     CDS             823..1746
                     /gene="yicL"
                     /locus_tag="ACHKG5_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0869549.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1750..2568)
                     /gene="nlpA"
                     /locus_tag="ACHKG5_003521"
     CDS             complement(1750..2568)
                     /gene="nlpA"
                     /locus_tag="ACHKG5_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0869550.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2790..3083
                     /gene="yicS"
                     /locus_tag="ACHKG5_003522"
     CDS             2790..3083
                     /gene="yicS"
                     /locus_tag="ACHKG5_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0869551.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3124..4314)
                     /gene="nepI"
                     /locus_tag="ACHKG5_003523"
     CDS             complement(3124..4314)
                     /gene="nepI"
                     /locus_tag="ACHKG5_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0869552.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            4548..4901
                     /locus_tag="ACHKG5_003524"
     CDS             4548..4901
                     /locus_tag="ACHKG5_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0869553.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4885..5208
                     /locus_tag="ACHKG5_003525"
     CDS             4885..5208
                     /locus_tag="ACHKG5_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0869554.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(5324..5776)
                     /gene="yicN"
                     /locus_tag="ACHKG5_003526"
     CDS             complement(5324..5776)
                     /gene="yicN"
                     /locus_tag="ACHKG5_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0869555.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5829..7162)
                     /gene="adeQ"
                     /locus_tag="ACHKG5_003527"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             complement(5829..7162)
                     /gene="adeQ"
                     /locus_tag="ACHKG5_003527"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            7337..9103
                     /gene="adeD"
                     /locus_tag="ACHKG5_003528"
     CDS             7337..9103
                     /gene="adeD"
                     /locus_tag="ACHKG5_003528"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP0869556.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(9149..10540)
                     /gene="uhpT"
                     /locus_tag="ACHKG5_003529"
     CDS             complement(9149..10540)
                     /gene="uhpT"
                     /locus_tag="ACHKG5_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0869557.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10678..11997)
                     /gene="uhpC"
                     /locus_tag="ACHKG5_003530"
     CDS             complement(10678..11997)
                     /gene="uhpC"
                     /locus_tag="ACHKG5_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0869558.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(12007..13509)
                     /gene="uhpB"
                     /locus_tag="ACHKG5_003531"
     CDS             complement(12007..13509)
                     /gene="uhpB"
                     /locus_tag="ACHKG5_003531"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0869559.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(13509..14099)
                     /gene="uhpA"
                     /locus_tag="ACHKG5_003532"
     CDS             complement(13509..14099)
                     /gene="uhpA"
                     /locus_tag="ACHKG5_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0869560.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14261..15288)
                     /locus_tag="ACHKG5_003533"
                     /pseudo
     CDS             complement(14261..15288)
                     /locus_tag="ACHKG5_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4151 a         4014 c         3585 g         3538 t
ORIGIN      
        1 aatagagtgt tcacaccaca tctggtatag aaaagcacag gctagcaaaa acctgaataa
       61 ccccgtactt acggagttat tcaggttaaa aatatccata acatcacttc tgatttttat
      121 tgcttacgac ggatataatt tctctggtca gctactaata tcatcatgct cacgcgttta
      181 atcaaactcg tgtttctcaa acggacatcg cgggacaatc ttctgcttct cccggcgcat
      241 tttttaccac cttcgctacc tgcattttga gcggaagatc cgcttttttc tggcccccgc
      301 acatatgcaa gtccagtttt aaatttatcc tttatttcat tctgaaacaa actgctcata
      361 attttctcca gtggaacctg catctcaggg agacatcact acagaatact tccccttatg
      421 cactgattta attttggtca tttagaataa cggcctgtaa agtctcaatg caacagatgg
      481 gtaacgctat tatgaggatc ctcgtcagta aattactggc aaggcactcc gataaataag
      541 aataggttcc gcaacaacgt tatatgcaaa tacctcacgc ttcggctaga ttatcgccgg
      601 ctctgttgac gaaattatgg ttgaaatctg gagctaccac acgttgttct ggctggcgat
      661 agggatgttg ggtattgcaa tgatttgctt gctgtttatt aaagatattt agctcaccag
      721 tctgggctaa gaaaaatcgg cgtggcgcaa actgagccat cctcgccatt tttgctatca
      781 tgcctgcata cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag
      841 gggatgctga acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgcgcgcaa
      901 tacatcatgg agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc
      961 gccggtttga ttctgctgac gctatcattt gttcatggtg ataaaatctt ttctattatt
     1021 aacaatcaca aagatgccat tagcctactg attttttccg tggttggcgc gctaaccgta
     1081 cagctcactt ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa
     1141 ttcctctcac cgactattat cgtcgcctgg ttctcactgg tgcgtaaatc ccgcccgggc
     1201 attctggttt tctgcgctat tttgacatcg ctgatcggga cttttttatt ggtgacacac
     1261 ggtaatccga cgtcattatc gatctctcct gccgcgttat tctggggcat tgcctcggca
     1321 tttactgctg cattctatac cacctatccc tcaacgctga ttgcccgcta tggcacgtta
     1381 ccggtcgtcg gctggagtat gctcattggc ggtctgatcc tgctgccctt ttatgccgga
     1441 caagggacaa actttatcgt taacggaagt ttgattctgg cgttttttta tttggtggtc
     1501 attggtacgt ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca
     1561 aaagccagca ttttaagctg tgctgaacca ttaagtagcg cgctgctctc tttgctgttg
     1621 ctggggatca ccttcacatt gccggactgg ctgggaacgc tgctgattct gtcatcggtg
     1681 attttgattt caatggattc ccgccgccgc gccagaaaaa taaatcgtcc ggcgcggcat
     1741 gagtgagaat taccagccag gcaccgcacc accgttaaag atggtttctg ccgctttagc
     1801 gacttcgggt gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc
     1861 ttcccgtgcc accagaatat tcacatacgg cgaattttta tcttcaataa atacgctgtc
     1921 gttaaccgga gaaagtcctg tttgctcgat gtaagtggtg ctgataatcg ctacatccac
     1981 tttcgggtcg tccagtacac gtggcaactg tgccccttcc agctccatta tctgcaaatg
     2041 gcgtggatta tcggttatat ccaacgcggt aggtaataac ccttttccct ctttcagagt
     2101 aattagcttc tctttttgca gcagtaaaag cgcgcgacca aggttagtcg ggtcgttggg
     2161 gatcgccact gtcgcacctt cttttatttg cgccacggtt ttgatttttt tggaatagcc
     2221 cgccataggg aagacaaacg tatttcccac cgctaccagt ttataaccat gcgtctgatt
     2281 atcttgttca agaaagggtc gatgctggaa gacgttcgca tcaagctcgc cattatttgt
     2341 cgcatcgttc ggtagcaacg aaccgctaaa tcctaccagt tcaacatcga ggccatattt
     2401 ctcttttgct acctttttag cgacttctgc gacatcttgt tctgcgccat taataacgcc
     2461 aactttaata tgcttttcat cgctgctgct ctggtcgcaa cctgccagca gaactcctgc
     2521 cagtaataat gcggcccccg cccgtagatg atgtgttgtc agtttcattt ttttatcctt
     2581 ttattaatga ttaccggact atagcggtaa ggtcgctgca gtttaaaaaa caaaacgcta
     2641 tcgataagaa taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac
     2701 gcaaacacct ttcgtacagc atgatgagag cggcaaaagc aaactacact tatgcttcat
     2761 tcgatgcaca tttgcagaag gttgccgcta tgaagccaac gatgctactc atgattaccg
     2821 tattcctgat ttttccagcc ctttctcagg ccgagtcgcc attcagttct ctacagtcgg
     2881 caaaagagaa aaccacggta ttgcaagatt tacgtaaaat ttgcacgcca caggcgtcat
     2941 tatcagatga agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac
     3001 gcgaggccat cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta
     3061 aggtagaatg cccggatatg taagaacggc aacaaccact gaccaacgct tctttacctg
     3121 atatcaggat ttcttcattt tcacctttgc agtcaccaac aatgcagtca gcaacatcaa
     3181 tgtgccggac aacatcagcg gcgaagtcag accaatgtta tccaacgcat aaccgccgat
     3241 tgccgcaccg caggtattgg caagctgaat aacggcaacc tgaatagatc cagccttttc
     3301 tgcctgatcg gccaatgagc gagtgatcca cgttgaccaa ccaacgggaa ttaaagcaaa
     3361 ggtcagcccc cagataatcg ccacaccagt agcaacaatt ttatcgcttc cccacagcgt
     3421 cagtaccagc gcgctcaggg cgagcacaaa cggtgcgccc gctaaggcca gtttcaccga
     3481 acgtttgaga ataaacgacg aaagcgatgt cccgacaaag ctggcgatac caaaactcag
     3541 cagcaccagg gttaagccat caacaccgaa tcccgccaga gtcatataca ctgggcgaat
     3601 ataagtgaaa aaagcaaact gtccggcaaa ggacataaag atggcgatca tcccggccat
     3661 cactcccgga cgttgtaata agcggaaagt attttgtttc tgatgcgaag gttcacctgg
     3721 cagcgaaggc agtgatttga taatccagaa aatacacagc acacccatcg ccgccgccgc
     3781 attaaagaca ttgcgccaac cgataagctc gcctaaaaaa cagcccaacg gcgcggcaat
     3841 caccagcgca atagaaaccg cgccgaagat caccgacagc gccttcggca ccgtacgcgg
     3901 cggcaccaga cgcatggtca gcgacgccga catcgcccag aacccgccca gcgccagccc
     3961 cagacaggca cgaccgatta aaagcaaact gaatgagtta gcaaaggaaa ccagcaagca
     4021 ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag cggcggtcag tggcctgaat
     4081 tgtctgggta ataaacaaac tggcaaacat tgccacaaag gcggtcacgg tcaccgattg
     4141 cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt ggcgtcaaca aactgacagg
     4201 caaaaactca acgataatca gacaggcgac gcaaaacgcc accgagaaaa cggctgacca
     4261 gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca ataaattcac tcatggtgtt
     4321 acccgtggca tgtttcaggg gaaaagccga ccactttaac attgaaatgt gtgacgcatt
     4381 taacgttttt gcaactttta cggtgttgtg ggaaaagctc gaggctgctc gaagaaataa
     4441 aaatcatagg acaaggtgat atgtattttc gcgattgtgt tgggaacaag ggataaagta
     4501 ttgcacttgg tgatatactt taataacagg acctcaacga tatacgcgtg tttaaaacca
     4561 aatggtttgc aagggaagcc aggtctcatg caataacaga tgaagagttg tgccgggcca
     4621 ttttagaaac agaacaaggt aaagctgatg cacttggcgg tggcgttttt aaaaagcgcc
     4681 ttcaccaaaa tcgtgagcgt gcaattattc tggcgaaagg ggtaagcaat tggttttaca
     4741 cgtttttata tgcaaaacag gatatgtcga acatcaatag tcaggaactg gctgggtttc
     4801 gtgaaattgc aaagcattat gccttcctga cgaaagccca actaacagca atgattaaca
     4861 ctaaagaact gacagagatc tgctatgact gcaaaaacta aatttaaaag tcctgcattt
     4921 gaggctattc acagcgcagc ggctggattg tccagcgtca atgctatttc tgctgaaacg
     4981 atgcgtactt ttgacaaagc ctgcctgacc agcgtgcaag acttacaacc tgttgagata
     5041 aaagcactgc gggaaaaatt aaaagtgagt cagcccgtct tcgctcacta tctcaatacc
     5101 agcgtttcaa ctgtacaaaa atgggaaagt ggcgctaaac gccccaatgg catttcatta
     5161 aaactgctca gcattgtgca aaaacacggg ttggaggtgc tgctgtaaat agaacgtaga
     5221 taatgcgttc acctactgtc agttccgata ccggatgcgg cgtgaacgcc ttatccaccc
     5281 tacaggtcag gcgcggtctg taggctgata agacgaagcg gttttaatgc agccagtgaa
     5341 cgccctcggc aggctgaaag aacgcgttat agcgcatctg aaaagcatta atatcctgca
     5401 tttcaggttc ggtttcgcgc agaaaaccca gcgccagcct gacctgagca tcagtaatgg
     5461 gagcggcaag gcgggctttt tgtaaaatcc gatgccattg cagcaggttc tgttcgctac
     5521 catcgacaat gacaatttgc cagatgagca acacctgcgc ggcgttttgc aggtgcgact
     5581 catccggacg atgcaaataa cgtaaaaatt cgtcaccggc tgactttccc atttgatcga
     5641 ccatcgactc cacgggtacg acatcgatat tcagccgatc gctgagacgg cgaatcgctt
     5701 ttctggcccc ggatgtcagc acccgaaaac caaccacaaa cactaccgcc agcgtggcga
     5761 gcattatcca gatcatgagt tctcctgcgt aatcagaact catgatatct ggaaaccctc
     5821 gccagttact aatccaccag aataatcttc agtgcaaaca gagctgcgac caccaccaca
     5881 cacaggttca gatcgcgcca gcgcccggtg cacactttca tgatgcagta cgacataaag
     5941 ccaagtgcaa tcccttcggt gatcgagaaa gtaaagggca tcatcaccgt ggtaacaaac
     6001 gcaggcaccg attcggtaaa atcatcccag ttaacgcgcg ccagacttga ggtcatcaac
     6061 acgccaacaa agattaacgc tccggcggtt gcgtaaggag gaaccatcgc caccagcggt
     6121 gagaagaaca taaccaacag gaacataacg ccgaccacaa ccgcagtcag ccccgtgcga
     6181 ccaccgactg ccacaccaga agtactttca atataggcgg taacagacga ggtgccgata
     6241 aacgcgcccg ccaccgaact acgctatcaa catatagcgc cttattcata ttggggaatt
     6301 taccgttact atctattaag ccagctttat cagttacacc aattaatgtt cctgatgaat
     6361 caaataggtt gatcagcata aaggagaaaa tgataccggc gagttcaagt gttaacgcgc
     6421 cgctcaaatc tacttcacca atgacgccgc taatatcagg cggaatggaa tagacgccgc
     6481 taaaatgaac atcaccgaaa aataatccac agcaagacgt caccacaata gaaacaagca
     6541 ccgcggcatg aaaatgacgt gatgacaaca cggttataat aaaaaaccct aaaataccta
     6601 acaacacgcc gtgagaactt aaatcgccaa tcatcaccag agtgtcttta ttggcgacaa
     6661 taacgccagt attttttaat cccattaagg cgataaataa tccgattccg ctggtgatac
     6721 caatacgtaa acttaatgga atgttggaga tcatccagta ccggatacga aatagcgtga
     6781 gcaaaaatag cccaattgcg ccccagaata tcgcgcccat cccggtctgc caggagatac
     6841 ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag ccccattgcc ggagccagcg
     6901 ccacgggtaa gttagcaaat atccccatcg caatactgcc gataccggca atcaaacagg
     6961 tggtaacaaa caccactttc gggtccattt gtgccgcgcc gaggatttgc gggttcacaa
     7021 aaacgatgta caccatggtt aaaaaagtgg tcatccccgc aatcaattct gtgcggacgg
     7081 tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt ccctgattca ttactcacgt
     7141 aatcggtatt gtcattattc atttttttgt ccattggagg agatttaatc ccttgctttt
     7201 gtaaaacaaa tgacaacaac ggttcagtga taattatcac atttcaattg cacattaatg
     7261 gatattcttt aataatcttg cgacatttct ttatgataaa taacaatcaa attaataaaa
     7321 tcaaaatgag aaaaatatga ataattccat taaccataaa tttcatcaca ttagccgggc
     7381 ggaataccag gaattgttag ccgtttcccg tggcgacgct gttgccgatt atattattga
     7441 taatgtctct attctcgacc tgattaatgg cggagaaatt tccggcccaa ttgtgattaa
     7501 agggcgttac attgccggtg ttggcgcaga atacactgat gctccggctt tgcagcggat
     7561 tgatgcccac ggcgcaacgg cggtgccagg gtttattgat gctcacctgc atattgaatc
     7621 cagcatgatg acgccggtca cctttgaaac cgccaccctg cctcgcggcc tgacaactgt
     7681 tatttgcgat cctcatgaaa tcgtcaacgt gatgggcgaa gccggtttcg cctggtttgc
     7741 ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtacccgc
     7801 tctggaaggc tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg
     7861 ggaccatccg caggttaccg gccttgcaga aatgatggac taccctggcg taattagcgg
     7921 gcagaatgcg ctgctcgata aactggatgc atttcgccac ctgacgctgg acggtcactg
     7981 cccgggtttg ggtggtaaag aacttaacgc ctatattgct gcgggtattg aaaactgcca
     8041 cgaaagttat cagctggaag aaggacgccg gaaattacaa ctcggcatgt cgttgatgat
     8101 ccgcgaaggg tccgctgccc gcaatctcaa cgcactggca ccgttgatca acgaatttaa
     8161 cagcccgcaa tgcatgctct gtactgatga ccgtaacccg tgggagatcg cccatgaagg
     8221 acacatcgat gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc
     8281 atatcgcgtc gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact
     8341 ggcacctggt aagcaggctg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca
     8401 gcaggtactg gtgaaaggcg aaccgattga cgcgcaaacc ttacaggcgg aagagtcggc
     8461 gaaactggca caatccgctc cgccatatgg caacaccatt gcccgccagc cagtttccgc
     8521 cagcgacttt gccctgcaat ttacgcccgg aaaacgctat cgggtcattg acgtcatcca
     8581 taacgaattg attacgcact cccactccag cgtctacagc gaaaatggtt ttgagcgcaa
     8641 tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa cgtctggctc cggcttgtgg
     8701 tttgctcggc ggctttggtc tgaatgaagg cgcgctggct gcgacggtca gccatgacag
     8761 ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg gcgctagcgg tcaatcaggt
     8821 gattcaggat ggcggcgggt tgtgcgtggt acgtaacggc caggtccaaa gccatctgcc
     8881 gttacccatt gccgggctga tgagcaccga cacggcgcag tcgctggcgg agcaaattga
     8941 cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt ttattcagat
     9001 ggcgtttctt tccctgccag tgatccccgc gctgaaacta accagccagg ggctgtttga
     9061 tggcgagaag tttgccttca ccacgctgga agtcactgaa taataaaaaa agcccggcgt
     9121 catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga atttttttct
     9181 cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag atacaaccaa
     9241 tcgcggcgat atccagcgca gcgaaggtgc ctgcccagcc ggtaaggccg aatactggcg
     9301 tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc aggtaagcga
     9361 aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag ccaacagcag
     9421 ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag agagaagcca
     9481 gatagatata ttcgttgctg gcatgttggt acacaccgag cgtggcgata atcagcgcca
     9541 gcgcgatgca ggccaccagg ccacggcgac cgttcgctag gtcagagagc cagccccaca
     9601 gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt
     9661 tagagagttt cagttcctgg aacgcgtaca cagttgacca ctggtcgata ccaatacgca
     9721 ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt ttcagcacat
     9781 actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg tcctcttcgc
     9841 tgatctcctc gccgaacagc tcttcagctt tgccgaggcc ataagattcc ggggagtcgc
     9901 tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg aagataaaca
     9961 tgccgatgac atggccatcg aacaggtaat ttgcgccgaa cagtgccaca ccagctgcgc
    10021 ctgcaccgcc aaggttgtga gaaatgttcc agaaaccgag gaacgtcccg cgtttacgac
    10081 gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc cccggtactc tggaaaaagc
    10141 cacttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg cccatactgg
    10201 cgctaaagcc cagcatacaa atggcagaca ggatcagcat gaacggcagg aattgtttgg
    10261 tgtttttgcc gtcggcgtag taggaaacca gcgttttacc cacgccataa gtgatggaga
    10321 aacccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgaa atcatatcgt
    10381 tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg ataaagacca
    10441 ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg agcggaaggt
    10501 ccagggtcgg cttgcggacc tggtttaaga aagccagcat gggttactcc tgaaatgaat
    10561 acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag
    10621 gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg agatgcatca
    10681 cgcttcgcgc ggtgtctggg cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc
    10741 ggcaatggcg ataaccacaa aaaatccgct ccagtgccag gtatcgagca ctttcgccag
    10801 cggccaacca gcaagcgacg cccccagata agcaaacaag ccgacaaacc ccgtcgccgc
    10861 ccctgccgcc tctttgtggg aacattctgc cgccgccatg ccgattaaca tctgcgggcc
    10921 aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg
    10981 catcagccac agcgagccga ctgaaagcaa aattccggca gcgaaaatca aattcatcgg
    11041 cccgcggttg ccgttaaaca gtttgtccga gccccaaccc gctaccagcg caccgataaa
    11101 tccgcccagt tcaaacatcg tcactgccgt attcgccgtg accagatcga cgcccagcgt
    11161 ctcggacata tacaaattgc cccagtcgtt gatcgccgcc cgaaccacat agaccagcac
    11221 atagcaaaac gaaagcagcc agatatacgg attcaacaac acatacttgg tgaggatctc
    11281 tttacgcgtc agccctgccc cttcttgttg ttgagcaatc tccagcgcgt cgtgtcgcca
    11341 ttcaccgacc gctggtaaac ctaacgcctg cgggcgatcg cgcagccgcc agcagagaaa
    11401 aatccccacg acgatcgcca tacaaccagc aatcatcatc ccggcacgcc agccgtaatg
    11461 cagcgcagct gctgccatca caatgggaat gagcgcaccg ccaacgttat gcgccgtgtt
    11521 ccataatgcc caccaaccac cgcgttcggt acgagaatac caggccgtta acagacgcgc
    11581 acacaccggt gaaccccagc cctggaaaaa ggcgttcagc acccagagca cggcaaacgc
    11641 ccatagcgac gtcgagaagc caaacagaat gttgatgatg cccgtggcga taagccctat
    11701 ccccataaaa taacgggcat ttgagcggtc gctgacaatg ccggagacaa acttcgacac
    11761 gccgtaggta atgtaaaaca gggtcgctaa caggccgata tcgctacggc tgagcacgcc
    11821 attagcaagg atttctggta cggcggcgtt aaaactttta cgcgtgaagt aaaacagcgc
    11881 gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgataac gggcatcaat
    11941 ttcatgttta tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat
    12001 caaaccttag acatagcgtt gaggtagaga aacgctgaca cgcgtgccgt gcagacagga
    12061 aatggtcaag gtgccgccca gcgccgttac gcgctcgcgc attccggtga ggccaaaacc
    12121 ttgttgcccg gaacccggcg gtaaaccgct gccatcgtct tcaatcacca gcatcaaccg
    12181 ttcatcctgc agccagcctt gcagggtgac cgcgctggca tcagcatgtt tcacaatgtt
    12241 attcagccct tcctggcaga cacgaaacag cgtcacgcgc tggttttcgc ttaacgctga
    12301 ctcttcgatt cgccattcga gatggctgac aatgccgcgc ccttccagct ccatttcccg
    12361 catcagtgag cggatggcct gctctagggt gagatcatcc aactggcgcg gacgtaaccg
    12421 ccctaacaaa cggcgcaccg cgtcgtacac gcccagcgac agttgttcga tgagctgccc
    12481 gctctgcttc acactggcgt tatctgccgc cagtcgctgg acaatgcccg cttgagtacg
    12541 aatggcagtg atggtctggc cgatatcatc atgcagctca cgcgccacat cacggcgcac
    12601 gctctcttca gtttctaaca aacgttcagc gagatgctga ttgcgcgcca gttccttttg
    12661 cagcgactgg ttaagttcac gcaaccgctg gatgccagcg cccagtaaca accctgtcag
    12721 actttgcacc agcagcgaga gcaataaatc cacaggatga tcgcgccagg tttgactggc
    12781 gatcagcgcg atggcgttca tcaatgtcgc tatcagcgcc ccttgccagc cgtagtgcca
    12841 ggccagcgcg ataatcggca gcgccaggca gaatggcgta aagcgcgaca gttcgtccgg
    12901 caagcccaac tggagccaga gactgataac aaacagcagc aagtaccaga ccagatgccg
    12961 cccgcgccag ttgatcggtt gagaaactaa cgacggcccg agcggcagcc aggtgttatt
    13021 ggcgagataa tgccagaaca ccagacatat cggggccagc gtcaggccgc cagttaaagt
    13081 taacagcagc gcattccacg actctttgcc gtgccaaagc cagggcagcg actgcaacaa
    13141 cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg cgccagtcac gttgatggcg
    13201 atagcgcgag atcagcgcca cgggcagtaa cgtcagtaaa ctaccgatca tcaataagga
    13261 gaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc agcagccact ccgctcccag
    13321 cagcaccggc cagtatccgc gcgggcattg cagcattagc cctagccgca gaccaaacgg
    13381 aaataacagc accgccatat cagggcgctc aaccaggtgc aggctgatac tccacaggca
    13441 aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc gggagaacaa
    13501 cgtcttcatc accagccatc aaacatacgg cgcgccagtt caacgtcgtt actgacgccc
    13561 agtttttcca tcagattggc gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg
    13621 gcggcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg ccgttcacgt
    13681 ttggttagtg ggtcctggcg accggatgcc agtttaatgg caatatccgg cgtcagataa
    13741 cagccgcctg tggcaaccgt atgcaccgca gcaatcagtt cgtcagggct acaacgcttg
    13801 gagagaaagc cacgcgcccc cgcgttaagc gcctgctcaa ccagcgccgg actgtcatga
    13861 acggagagca taattgtcgc catacctttc ggcagctggc ttagcagctc cagaccggag
    13921 atatcgggca tggagatatc gcaaatacac acctgcacac cgcgccccgg cagccccgcc
    13981 agcgcctcgc gccccgaacc aaactcggca actacttgca aatcaggttc cagccccagc
    14041 aactgcgcaa agccggagcg gacgatgagg tgatcgtcta taagggcaac ggtgatcatg
    14101 attgggtcct ggcggataaa aaacgcgcat accttaacga taagcgcgat gttgttcaag
    14161 ttggatatgg ggtatgtatt ggcttaatga tgttgagctt atcgtcaaaa cgcttacctg
    14221 agaggtaagc gtttggtgcg tgttattgag aaaaagcgaa ttatttgtag gttgcaatcg
    14281 taatattgcc tgagaaattt ctccttagat tgcggatatc attatccctt gcccaataat
    14341 catctttgtg tctataactc tcatcggaat gtacataggg agaatcatct tcagcgtcgt
    14401 tatggataat attttcgcct ttagtggtat agatataatt agcaacatca aaggtgtcat
    14461 tcgcgttatt tacgactgcg cctaagacgc aaagggtttg agtgaatccg tattctgaga
    14521 aatcgacatg ctgatggtaa tttctaccat tcaaagcaac aaaactcgga caaacatcta
    14581 ttgtatcgcc aacagacgta gcggcgcgac gcacatcaag tgcagcgtta tcgttagcaa
    14641 catgaaatgc ttgtgaacag gcataaaaca aatttctaat cccggtacga ctggataaac
    14701 aagaaataat ttttgctatt ttttctggct ttataccatc ctgtactaaa gaatctctca
    14761 ccagatcaca ttcaccaggt gtgaaaacaa ctttcttgaa tatttcctca taaacatcat
    14821 aagaaaaata ttcagagaga gtgaaagatt cggtgggatg tgttaaatct gtaatttttt
    14881 gctaagtaat tcacttatag tgctgtttat tttttcaccg caacataaaa caaatgaact
    14941 tccctccaga acttgccttt ctgcatctac aaggagtgag ctaaattcat cctcatgaag
    15001 cttaagtttt tttatacctg attgatgtaa atatttttct ggaattaaat ctttttgttc
    15061 ccttataagc tctatcattc ggcgttcggt ttgcgtatca aaagagtatt tcatgttata
    15121 gttattttta gcaacttcca gcttaagagc atttgcagca actgttaatt ctgagatttg
    15181 atctttttca aattgatcgt gatctgataa taaccttata aactctttta catttcgacc
    15241 actaaaatcc atatccctac actttttatc ataacacttt gtttcgcc
//
LOCUS       DAZGXS010000108        14929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGXS010000108 DAZGXS010000000
VERSION     DAZGXS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            78..347
                     /locus_tag="ACHKG5_003534"
     CDS             78..347
                     /locus_tag="ACHKG5_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0869561.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHKG5_003535"
     CDS             316..1434
                     /locus_tag="ACHKG5_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0869562.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHKG5_003536"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHKG5_003536"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0869563.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHKG5_003537"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHKG5_003537"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0869564.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHKG5_003538"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHKG5_003538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0869565.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHKG5_003539"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHKG5_003539"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0869566.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHKG5_003540"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHKG5_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0869567.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHKG5_003541"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHKG5_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0869568.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHKG5_003542"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHKG5_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0869569.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHKG5_003543"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHKG5_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0869570.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHKG5_003544"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHKG5_003544"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0869571.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGDAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHKG5_003545"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHKG5_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0869572.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHKG5_003546"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHKG5_003546"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0869573.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3730 a         3877 c         3809 g         3513 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aatctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgacctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtgatgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctac cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgata
//
LOCUS       DAZGXS010000109        14919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGXS010000109 DAZGXS010000000
VERSION     DAZGXS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            139..381
                     /gene="yjbE"
                     /locus_tag="ACHKG5_003547"
     CDS             139..381
                     /gene="yjbE"
                     /locus_tag="ACHKG5_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP0869574.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            495..1133
                     /locus_tag="ACHKG5_003548"
     CDS             495..1133
                     /locus_tag="ACHKG5_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0869575.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1130..1867
                     /locus_tag="ACHKG5_003549"
     CDS             1130..1867
                     /locus_tag="ACHKG5_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0869576.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEIPDALNADILQTLTQRIPQ"
     gene            1867..3963
                     /locus_tag="ACHKG5_003550"
     CDS             1867..3963
                     /locus_tag="ACHKG5_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0869577.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4010..4288)
                     /locus_tag="ACHKG5_003551"
     CDS             complement(4010..4288)
                     /locus_tag="ACHKG5_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869578.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4289..4432
                     /locus_tag="ACHKG5_003552"
     CDS             4289..4432
                     /locus_tag="ACHKG5_003552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869579.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4558..4968
                     /gene="psiE"
                     /locus_tag="ACHKG5_003553"
     CDS             4558..4968
                     /gene="psiE"
                     /locus_tag="ACHKG5_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0869580.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHKG5_003554"
     CDS             complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHKG5_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP0869581.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHKG5_003555"
     CDS             complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHKG5_003555"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0869582.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHKG5_003556"
     CDS             complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHKG5_003556"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0869583.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHKG5_003557"
     CDS             complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHKG5_003557"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0869584.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11017..12132
                     /gene="malK"
                     /locus_tag="ACHKG5_003558"
     CDS             11017..12132
                     /gene="malK"
                     /locus_tag="ACHKG5_003558"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0869585.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12204..13544
                     /gene="lamB"
                     /locus_tag="ACHKG5_003559"
     CDS             12204..13544
                     /gene="lamB"
                     /locus_tag="ACHKG5_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0869586.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13695..14615
                     /gene="malM"
                     /locus_tag="ACHKG5_003560"
     CDS             13695..14615
                     /gene="malM"
                     /locus_tag="ACHKG5_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0869587.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3681 a         3838 c         3933 g         3467 t
ORIGIN      
        1 ttgatattca ttccaattat cctaaaaccc catctctaat tccccgcgcc gtaattcgca
       61 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
      121 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
      181 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca
      241 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
      301 ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
      361 acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc
      421 tgtggctaga agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc
      481 tctggagaag agtcgtgaag cgacctgcat tcattcttat ctgcctgcta ttacaggcct
      541 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
      601 gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc
      661 ttaatggtgg gccgcagttg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
      721 gggtcactca ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc
      781 ttggcggcga caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg
      841 cacaaatcat tgatggcgca acctggatgc gcacgatggg ctggaccgag taccagcagg
      901 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccggtacc gttaaggtcg
      961 gcagcgatga aaccgcggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     1021 ggcataaccg ctattggatc gacagcggag ggcaaattcg ccagtcggaa cagtatctcg
     1081 gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     1141 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     1201 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     1261 cgatctggta ggccaaccgc ggctggcaaa cagctggtgg cctggtgcgg tgattagcga
     1321 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     1381 acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac gccagcaaat
     1441 tcaggcgttg aaggtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     1501 tgctgaacgc gctaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccacc
     1561 gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aatcagccat tcactcccgg
     1621 tcgcgacgtg gcgagctatc tctccgggca aagtctgctt agcggtgcgg atcgtagcta
     1681 cgcgtgggtg gtttacccgg acggacgcac acaaaaagcg ccggtggctt actggaacaa
     1741 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg
     1801 gagtgagata cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     1861 tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     1921 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     1981 ttattacaaa cgcccacggc tcgcatggcg cgggaagggg agttgagtct gaactatcgc
     2041 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     2101 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     2161 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     2221 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     2281 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgcttgg cctgggctgg
     2341 ggatatttgg gcacaagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat
     2401 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     2461 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     2521 aaactggagt atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa
     2581 agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     2641 tatgaacgtg gcaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg
     2701 cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     2761 ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc
     2821 gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     2881 cgtgattcgc gcgaagggat cgttcgtgct aatcggatcg tgatgaatga tctgccggat
     2941 gggatcaaga cgatccgcgt tacggaaaat cgccttaaca tgccgcaggt gacgacggaa
     3001 acggatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag
     3061 ctggcgcaaa aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc
     3121 gacaaatcgc gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     3181 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     3241 catctgctga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     3301 tacaccaatc cgccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat
     3361 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     3421 ggcttctacg gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca
     3481 gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt
     3541 aaacagcgcg actggcgtag tgcaaaggat atgatgaaat tcaccgacta cagcgtgaaa
     3601 accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc
     3661 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt
     3721 gacagcggcg tggtggtggg cggctatgcc acgatcacca acgtttcgaa agaggagtac
     3781 ggcgaagggg acttcaccaa aggcgtgtat gtctctgtac cgttggatct cttctcgtct
     3841 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag
     3901 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc
     3961 taagtcatgg gaaaggtgcc agttttcgca atcactggca cctttattct taatggcctg
     4021 ttgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac
     4081 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     4141 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     4201 cagcataagc aggaagagca gagactgata acatcaaagc agcaaaataa ggcttcattt
     4261 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     4321 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     4381 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     4441 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     4501 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4561 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4621 cttggcctgc tgtgcctcgg ccttattttg gttgtcttcc tcggcaaaga aacggtgcat
     4681 ctggctgatg tgctgttcgc gccagaacaa gccagcaaat atgagctggt agaaggactg
     4741 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4801 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     4861 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4921 gtggtcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     4981 ccgaactggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5041 tcgtttccgg ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga
     5101 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     5161 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5221 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttacctcgaa
     5281 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5341 aggcggcaac atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa
     5401 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5461 gcttacgacc aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa
     5521 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5581 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     5641 taacgccgat gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5701 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5761 ttttgcgcag gataccttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac
     5821 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5881 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgc gcaataaaat
     5941 agtttacgca gtaaactaaa agctgcccga aaataatcgc aaactggtta aatgaaacca
     6001 gtttcccgcg aatatgagca ggagcaagtt ccgcaatata cattggtgaa agcattgagg
     6061 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
     6121 ccagataaac aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6181 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac
     6241 cgaagcggtt actgcaataa ccaccgaggg cacctccgat gatgcaacca atcagagcgc
     6301 tggccacgca aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6361 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6421 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6481 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6541 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6601 gatcttaatt gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga
     6661 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6721 atggaaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6781 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6841 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6901 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     6961 aaagtcaccc cacaggtagt tttgcggatt gaggtattgc tgcatcccca cggccagggt
     7021 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7081 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7141 gaaggcctgc cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat
     7201 ggtttcgaaa tagcctttga tggtccagac gtgcagcgca atcccaccca gatacgcgaa
     7261 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa
     7321 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7381 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7441 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7501 cagcagcacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc
     7561 taatgccagt ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa
     7621 gttcccctgg cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat
     7681 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     7741 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7801 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag
     7861 tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta
     7921 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     7981 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8041 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8101 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8161 tacggatagc ccagccaggt attgacgata atcagcatcg ttcgggcggt ggtcggatcg
     8221 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag
     8281 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8341 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     8401 gccagaacca tgccgactgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8461 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgagtaaag
     8521 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8581 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8641 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8701 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8761 agcgcctgac gattctgggt aatcacgcgt agatttgcgc gttcgccttc gggctgggcg
     8821 gtcgtttctt tcagttgcag tttttgctct ctgccaaatt taaaagcgtc ggagaggtaa
     8881 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga
     8941 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt
     9001 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9061 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9121 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9181 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9241 agcagaccta acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9301 acatccatgg ggttcttcct cattccagga cggataaggc tttcatgcct tatccgacaa
     9361 caactgcctg atgcgacgct gacgcgtctt atcaggccta catacgtttc ggttttgtag
     9421 gccggacaag gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     9481 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
     9541 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
     9601 tgggcgtttt ccatggtggc tgcaatacgt ggatctttcg ccaactcttc ctcgtaagac
     9661 ttcagcgcta cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc
     9721 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     9781 ccggcgctca gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc
     9841 gttacaccat aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgata
     9901 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
     9961 ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg
    10021 ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc
    10081 ccgtcagcag caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg
    10141 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc
    10201 gggttcggta gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa
    10261 gcaatcagct tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg
    10321 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
    10381 tcgtgcgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc
    10441 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
    10501 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
    10561 ccttcttcga ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat
    10621 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
    10681 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
    10741 gcccctacgc cccaccgttg ctttgtgtga tctctgttac agaattggca gtaatgtgga
    10801 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
    10861 ctcggtttag ttcacagaag ccgcgttctc atcctcccgc ctcctccccc ataaaaaagc
    10921 tagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
    10981 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
    11041 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
    11101 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
    11161 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
    11221 gacactccgc cagcagaacg tggcgttggt atggtgtttc agtcctacgc gctctatccc
    11281 cacctgtcag tagcagaaaa catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag
    11341 gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctac aactggcgca tttgctggat
    11401 cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg
    11461 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
    11521 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
    11581 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
    11641 gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt
    11701 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc
    11761 accgcaatcg atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg
    11821 ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa
    11881 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    11941 caactcggca acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg
    12001 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    12061 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    12121 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    12181 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    12241 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    12301 gttccggtat tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg
    12361 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    12421 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    12481 tcgcgcaaca gaatgactgg gaagctaccg acccggcctt ccgtgaagca aacgtgcagg
    12541 gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg ggcaggtaag cgcttctacc
    12601 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    12661 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg
    12721 aagcaggtgg ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg
    12781 cgaacgacgt tttcgatgtg cgtttagcgc agatggaagt caacccgggc ggcacattag
    12841 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    12901 catcgaaaga cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta
    12961 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    13021 agggttctgg cgttgcattt gataacgaaa aatttgccta caatatcaac aacaacggtc
    13081 acatgctgcg tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    13141 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    13201 tcggtattcg cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct
    13261 acgacaacgt cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg
    13321 cacaacaatg gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa
    13381 cctacgccaa gtgggatgag aaatggggtt acgactacaa cggcgatagc aaggttaacc
    13441 cgaactacgg caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca
    13501 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    13561 ggataaggcg tttacgccgc atccggcata aaacaggttg tcattatctg aaaggggcga
    13621 aagcccctct gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt
    13681 ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt
    13741 tactggcaag cgcgcctgga atcagccttg ccgatgttaa ctacgtaccg caaaacacca
    13801 gcgacgcgcc agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc
    13861 aatctaaaac ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca
    13921 tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc
    13981 tgaccagcga agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc
    14041 agaacatgac cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg
    14101 tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa
    14161 aactttatgt tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg
    14221 acccggctaa agcctatgcc aaaggcgttg gtaactcgat cccggatatt cccgatccgg
    14281 ttgctcgtca taccaccgat ggcttactga aactgaaagt gaaaacgaac tccagctcca
    14341 gcgtgttggt cgggccttta tttggttctt ccgctccagc tccggttacg gtaggcaaca
    14401 cggcggcacc agctgtggct gcacccgctc cggtgccggt gaagaaaagc gagccgatgc
    14461 tcaacgacac ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg
    14521 ttgataaggc gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt
    14581 ccacctttat cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc
    14641 aacaactggt gcgtctcctg gcgcaccttt ttttatgctt cattccaggg atatgagcga
    14701 ttttttatag taactcactt cttcttcact aagaatatcc attatctcaa tgcattatca
    14761 gagattcttt tcctttcgcc ggtagtgtct ggacgctcag gctacttttt ccagttattt
    14821 catttctgta atgcagaggt tttatgttag gtcatatctc aaagtttgac ggcaataact
    14881 ctttgataaa acatggtgtg gtgcaaggaa ataatatag
//
LOCUS       DAZGXS010000110        14915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGXS010000110 DAZGXS010000000
VERSION     DAZGXS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..134)
                     /locus_tag="ACHKG5_003561"
     CDS             complement(<1..134)
                     /locus_tag="ACHKG5_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869588.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     L"
     gene            complement(365..1339)
                     /gene="yajO"
                     /locus_tag="ACHKG5_003562"
     CDS             complement(365..1339)
                     /gene="yajO"
                     /locus_tag="ACHKG5_003562"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0869589.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1394..3256)
                     /gene="dxs"
                     /locus_tag="ACHKG5_003563"
     CDS             complement(1394..3256)
                     /gene="dxs"
                     /locus_tag="ACHKG5_003563"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0869590.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3281..4180)
                     /gene="ispA"
                     /locus_tag="ACHKG5_003564"
     CDS             complement(3281..4180)
                     /gene="ispA"
                     /locus_tag="ACHKG5_003564"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0869591.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(4180..4422)
                     /gene="xseB"
                     /locus_tag="ACHKG5_003565"
     CDS             complement(4180..4422)
                     /gene="xseB"
                     /locus_tag="ACHKG5_003565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0869592.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4628..6076
                     /gene="thiI"
                     /locus_tag="ACHKG5_003566"
     CDS             4628..6076
                     /gene="thiI"
                     /locus_tag="ACHKG5_003566"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP0869593.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(6130..6720)
                     /gene="yajL"
                     /locus_tag="ACHKG5_003567"
     CDS             complement(6130..6720)
                     /gene="yajL"
                     /locus_tag="ACHKG5_003567"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0869594.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6683..7594)
                     /gene="panE"
                     /locus_tag="ACHKG5_003568"
     CDS             complement(6683..7594)
                     /gene="panE"
                     /locus_tag="ACHKG5_003568"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0869595.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7762..8253
                     /gene="yajQ"
                     /locus_tag="ACHKG5_003569"
     CDS             7762..8253
                     /gene="yajQ"
                     /locus_tag="ACHKG5_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0869596.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8381..9745)
                     /gene="yajR"
                     /locus_tag="ACHKG5_003570"
     CDS             complement(8381..9745)
                     /gene="yajR"
                     /locus_tag="ACHKG5_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0869597.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(9894..10784)
                     /gene="cyoE"
                     /locus_tag="ACHKG5_003571"
     CDS             complement(9894..10784)
                     /gene="cyoE"
                     /locus_tag="ACHKG5_003571"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0869598.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(10796..11125)
                     /gene="cyoD"
                     /locus_tag="ACHKG5_003572"
     CDS             complement(10796..11125)
                     /gene="cyoD"
                     /locus_tag="ACHKG5_003572"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0869599.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11125..11739)
                     /gene="cyoC"
                     /locus_tag="ACHKG5_003573"
     CDS             complement(11125..11739)
                     /gene="cyoC"
                     /locus_tag="ACHKG5_003573"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0869600.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11729..13720)
                     /gene="cyoB"
                     /locus_tag="ACHKG5_003574"
     CDS             complement(11729..13720)
                     /gene="cyoB"
                     /locus_tag="ACHKG5_003574"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0869601.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13742..14689)
                     /gene="cyoA"
                     /locus_tag="ACHKG5_003575"
     CDS             complement(13742..14689)
                     /gene="cyoA"
                     /locus_tag="ACHKG5_003575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0869602.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3715 a         3931 c         3822 g         3447 t
ORIGIN      
        1 agaaaaaaat cacgaatttt gttccacagc ctttcaataa gattttgatt taaatcttgc
       61 ttcattgagt ttcttaaatc tgcaaccggt acatcagtaa acaggatcgg ttgtttggtg
      121 atggttttca tcatgattgt aatcctctta cgttgactaa ataagacagt tttaggggat
      181 attacctgga gaaactatca aaaatcgctc aaagggatga agacgatgaa tagctgcaaa
      241 ctcattcacg aagaatttct cttgcagagg tggattatgg ttacagaagg tgatgggagg
      301 tacgttgtcc ccatcatcgc agacgatggg gaattcagca gataaaatgg gtgaaaagcg
      361 aaatttactt aaatcctacg accggatgcg gtttatacgg tgtttccagt tcagcaatct
      421 gttccggctt caaagtgata tccaccgcgt tcagcagctc atcaagctgt tcttcccgcg
      481 aagttccgat aatcggtgcg gcaatgcccg gtttactcaa caaccaggcc agcgcaactt
      541 gtgctcgtgt tgcaccgagt tcttcactga cgcccgttaa ccgctctgcg atctgcgcgt
      601 cattttcatc gctttcttta tagagatttt tccccacctc atcagacacc agtcgtgcgg
      661 tagtttctcc ccacggacgc gtcagtcgcc cccgcgccag cggactccac ggaatgaccg
      721 ccacgccttc ctgataacac agcggcagca tctcgcgctc ttcttcgcga taaatcagat
      781 tgtagtgatc ctgcatactg acaaactgcg cccagccgtg ctgtttttgt agttccagtg
      841 cctgagcaaa ctgcgaagcg tgcatggatg acgcgccgat ataacgcgct ttcccggctt
      901 ttaccacgtc gttgagggct tccagcgtct cttcgatcgg cgtgttgtaa tcccagcgat
      961 gaatttgcag gatatcgaca taatccatgc cgagacgtcg caggctgtcg tcgatagagc
     1021 gcaaaatttg tgcacgggat aatccttccg gtaaatcacc aacgcgatgg aacactttgg
     1081 tggcaacgac cacgtcttca cgacgggaga aatcccgcag tgcgcgaccg acgatctctt
     1141 cgctgctgcc atcggaatag ctattggcgg tatcaaagaa atttatgccg ccttccagtg
     1201 cgcgtttaat gatgggacgg ctgctctctt ccggcagtgt ccatgcgtga ttaccacgat
     1261 ctggctcgcc aaaggtcata cagccgaggc aaagtcggga aacgcgaagg tcggtttttc
     1321 ctaaggggtt gtattgcatg ctgccactcc tgccatgaat aatttcttaa gcatagcagg
     1381 agtggagtag ggattatgcc agccaggcct tgattttggc ttccataccg gcggcatcga
     1441 ggccgagttc ggcgcgcatt tcttcctgag ttccttgcgg aataaagaag tccggcaggc
     1501 caatgttcag cacgggtact ggtttacgat gggccatcag cacttcgttc actccgctgc
     1561 ctgcaccgcc cataatggcg ttttcttcta cggtaaccag tgcttcatga ctggcggcca
     1621 tttccagaat taacgtttca tcaagcggtt tcacaaaacg catatcgacc agcgtggcgt
     1681 tcagcgattc ggcgactttc gccgcttctg gcatcagcgt accaaagtta aggatcgcca
     1741 gtttctcgcc acgacgcttc acaatgcctt tgccaattgg tagtttttcc agcggcgtca
     1801 gttccacgcc gaccgcgttg ccacgcgggt agcgcaccgc tgacgggcca tcgttatagt
     1861 gatagccggt atagagcatc tggcgacatt cgttttcatc gctcggggtc ataatgacca
     1921 tttccggtat gcagcgcagg taagagagat caaaagcgcc ctggtgggtt tgaccgtcag
     1981 caccaacaat gcccgcgcgg tcgatggcga acaggaccgg gagtttttga attgccacgt
     2041 catgcagcac ctgatcatag gcgcgttgca ggaaggtgga gtaaattgcc acgatcggtt
     2101 tgtagccacc aatcgccaga ccggcggcaa aggtcaccgc gtgttgctcg gcaatcgcta
     2161 catcaaaata gcgatccggg aatttacgtg aaaactcgac catgccggaa ccttcacgca
     2221 tcgccggagt aatcgccatc agtttgttat ctttcgctgc cgtttcgcac aaccagtcgc
     2281 caaagatttt tgaatagctt ggtaaaccac cgctactttt cggcaaacaa ccgctggagg
     2341 gatcaaattt aggcacggcg tggaaggtga ttgggtcttt ttctgccggt tcataaccac
     2401 gacctttttt ggtcatgata tgcaggaact gcgggccttt caggtcgcgc atgttcttca
     2461 gcgtggtgat aagccccagc acatcgtgac cgtcaaccgg gccgatgtag ttaaagccca
     2521 gctcttcaaa caacgtgcca ggcaccacca tgcctttaat atgttcttcg gtacgtttga
     2581 gcagctcttt aattggcgga acgccagaga aaactttttt cccgccttcg cgcagtgaag
     2641 agtaaagctt accggaaagc agctgcgcca gatggttgtt aagcgcgcca acattttcgg
     2701 aaatcgacat ttcattgtcg ttgaggacca ccagcatatc aggacgaata tcgcccgcgt
     2761 gattcatcgc ttcaaacgcc atgcctgcgg taatcgcgcc gtcgccaatc acacagacgg
     2821 tgcggcgatt tttgccttct ttctcggcag caaccgcaat accaattccg gcactgatgg
     2881 aggttgatga atgcccgacg cttaatacgt catattcgct ttcgccgcgc cacgggaacg
     2941 ggtgtagacc gcctttctga cggatggtgc cgattttgtc gcggcgtccg gtcaaaattt
     3001 tatgcggata agcctgatgc cccacatccc aaatcaattg gtcaaacggg gtgttataga
     3061 catagtgcag cgccacggtc agttcgaccg tgcccagccc ggaggcgaag tgcccgctgg
     3121 aacggctcac gctgtcgagt aaatagcggc gcagttcgtc gcagagtttc ggtaaactct
     3181 cttttggcaa cagtcgtagc tcctgggtgg agtcgaccag tgccagggtc gggtatttgg
     3241 caatatcaaa actcatcagg ggcctattaa cacttattgt ttatttatta cgctggatga
     3301 tgtagtccgc tagcgcttcc agtgccgaag tatcgatcga ctgttcagcc agttgtttca
     3361 gcgactggcg ggcatcgtcg atcagatcct gggctttctt ccgggcttgc tcaagaccca
     3421 ggagtgcagg gtaggtactt ttgccaagct gctggtcggc accctgacgt tttcccaacg
     3481 ttgcagtatc tcccaccaca tccaggatgt catcctgaac ctggaaggca aggccgatgc
     3541 tctctgcgta cttgtcgagt actggtagag cacgacgtcc tttatctccg gcgcttaatg
     3601 caccaaggcg aacggcggcg cgaattaatg cgcctgtttt atgacgatga atacgctcaa
     3661 gcgcgtccag aggtacgtgt ttgccttccg cgtctaaatc caacgcctga ccaccacaca
     3721 ttccggcaat accgctggcg ctcgccagtt cagaaatcat cgaaattctg tcgcgatccg
     3781 acacttctgg catatcagca tcgcttaaaa tcgagaacgc cagcgtttgt aaagcgtcgc
     3841 cagcgagaat cgcgttcgct tcgccaaact tcacatggca ggtcggcaag ccgcgacgca
     3901 gatcgtcatc atccatcgcc ggtaaatcat catgaattaa tgagtaagcg tggatacact
     3961 ctacggcggc agcgggtgcg tccagcgtgt ttgtgctaac gccgaacata tgaccggtgg
     4021 cataaaccag gaaaggtcgc aggcgcttac cacctaataa tgcgccatac tgcatggttt
     4081 cgaccacggg agtgttctga aagggcagtg gggcgataaa acggctcagc gcctggttgg
     4141 cctgcttaac gcaggcttcg agttgctgcg gaaagtccat tactcattgt ccggtgtaaa
     4201 aggggttaga gaggcgtctt cgttgtcaga cagcagaatt tgtacgcgct gttcggcttg
     4261 ttgtaatttg gcctgcccct gacgtgccag ctgcacgccg cgttcgaact cgttcagcgc
     4321 ctcttccagc ggcaggtcgc cactttccag acgggttaca atctgttcca gctcgctcag
     4381 cgccttttca aagctggcgg gcgcctcatt tttcttcggc ataatgaatg tctgactctc
     4441 aatatttttc gccccgtcat ggtaacggac tcagggcaaa tagcaaataa cgcgcaatgg
     4501 taaggtgatg tgcgcagcaa agcgatgtta gtggtatact tccgcgcctg gatgcagccg
     4561 caggtgtggg ctgctgtatt tttccctata caagtcgctt aaggcttgcc aacgaaccat
     4621 tgccgccatg aagtttatca ttaaattgtt cccggaaatc accatcaaaa gccaatctgt
     4681 gcgcttgcgc tttataaaaa tccttaccgg gaacattcgt aacgttttaa agcactatga
     4741 tgagacgctc gctgtcgtcc gccactggga taacatcgaa gttcgcgcga aagatgaaaa
     4801 ccagcgtctg actattcgcg acgccctgac ccgtattcct ggtatccacc atattctcga
     4861 agtcgaagac gtgccgttta ccgacatgca cgatattttc gagaaagcgt tggttcagta
     4921 tcgcgatcag ctggatggca aaaccttctg cgtacgcgtg aagcgccgtg gcaaacatga
     4981 ttttagctcg attgatgtgg aacgttacgt cggcggcggt ttaaatcagc atattgaatc
     5041 cgcgcgcgtg aagctgacca atccggatgt gactgtccat ctggaagtgg aagacgatcg
     5101 tctcctgctg attaaaggcc gctacgaagg tattggcggt ttcccgatcg gcacccagga
     5161 agatgtgctg tcgctcattt ccggtggttt cgactccggt gtttccagtt atatgttgat
     5221 gcgtcgcggc tgccgcgtgc attactgctt ctttaacctc ggcggcgcag cgcatgaaat
     5281 tggcgtgcgt caggtggcac attatctgtg gaaccgcttt ggcagctccc accgcgtgcg
     5341 ttttgtcgct attaatttcg agccggtagt cggggaaatt ctcgagaaaa tcgacgacgg
     5401 tcagatgggc gttatcctca aacgtatgat ggtgcgtgcc gcgtctaaag tggctgaacg
     5461 ttacggcgta caggcgctgg tcaccggcga agcgctcggc caggtgtcca gtcagacgct
     5521 gaccaacctg cgcctgattg ataacgtctc tgatacgctg atcctgcgtc cgctgatctc
     5581 ttacgacaaa gagcacatca tcaacctggc tcgccagatt ggcaccgaag actttgcccg
     5641 tactatgccg gaatactgcg gtgtgatctc caaaagcccg acggtgaaag cggttaagtc
     5701 gaagattgaa gcggaagaag agaaattcga cttcagcatt ctcgataaag tggttgaaga
     5761 agcgaataac gttgatatcc gcgaaatcgc ccagcagacc gggcaggaag tggtggaagt
     5821 ggaaaccgtc aatgacttcg gcccgaacga cgtgattctc gatatccgtt ctgtcgatga
     5881 acaggaagat aagccactga aagtcgaagg gattgatgtg gtttcactgc cgttctacaa
     5941 gttgagcacc aaatttggcg atctcgacca gaacagaacc tggctactgt ggtgtgagcg
     6001 cggggtaatg agccgcctgc aggcgctcta tctacgcgaa cagggcttta agaatgtgaa
     6061 ggtgtatcgt ccgtaatttg tggtttttac gtcgcatctg gtcagatgcg acgtttgccg
     6121 catccgacac tactcgtaat aattataaat ccctgccgcc atcaccagtt gtgatgccac
     6181 ttcatgggct ttctcgcgcc caaccagcag gtcgataatt ttcagaccaa agtcgatagc
     6241 tgtacccggc ccctggctgg tcagcaattt tacccgtgca tcccagacga cgcgcttgtc
     6301 ctgccattgt tcagcgggaa ttttgtcttt cagcgtcggg aagccggtca tattgccaat
     6361 cggaaagata tcgtgcggca ccagcacggt ggctggcgcg gcgcaaatag ccgcgacgat
     6421 acgcccggaa cggtggaact gtttaacggt ttcaaccagc agggtactat cgcgaaaaca
     6481 ctccgcgcct ttaatgccac caggcagcac gatcacgtca tattcgccat cagccacttc
     6541 gaccagcggc gcatccgcca gcagcttcac gccgcgcgag caggtaatcg ccaggttgcc
     6601 atcgctggct acgctggcag tggtgacttt gataccgccg cgaatcaaga ggtcgatcgt
     6661 ggtgacggct tcagtctctt cactaccagg ggcgaggcaa accagtgccg atgcgctcat
     6721 attcactctc ctttcttttt accatttcaa acaggcgggt gttttccggt acggcaatcc
     6781 catgcgcgcg ggcgcggcgt aagagaaaac cattgatata gtcgatttcg gtgtggcgca
     6841 gcgtgcggat atcctgcaac atcgacgaga tattttccgc tgtggcatca atcacctgca
     6901 tcacgtaatc acgtaaatct tccgctgaag tatgatgccc ttcgcgttcg atcactgccg
     6961 cgacttcttc gcatatttgc ataatttctt gcggatgatg acgtaattca ccgttcgggc
     7021 aattccagat ggcagtcagt ggattaatca cacagttgac tgccagcttg cgccacagct
     7081 cggcgcgaat attgttatgc caggcaacgt caggcaacac ggtttgcaat atatccgcca
     7141 gataactgta atcgccgtcc tgttgccgtg ctggaccaat atgcgtgata ccgtttgcca
     7201 catgaataat gacattgccg tcgcggcagg cggcatgggt ggtggtgccc atcagtaatg
     7261 gctgctgaat gttttgcaat tcttcgatgg tgcccatgcc gttgtgaatt aacagtattg
     7321 gcgtagttac aggcagtgtg gacgcgaggc ttttgacggc atcggaaacc tgccatgctt
     7381 tcagcgtcac caggagcaga tcgctggtgg caagaaaatc gggatcgttg gcggtcagcg
     7441 attcgttaaa tatcgaacca tcagtctcaa ccagattcac actacaataa ggttgcggta
     7501 cgcgcagcca gccctgaact tcgtgaccct gtttacaaag tgctgtaagc cataattgcc
     7561 ctaaggcacc gcatcccaat acggtaattt tcattgttcc tcctcacccg caaccactcc
     7621 gggtgttcaa taaggctatc ccttaattgt gcatgctgtt gcgactatgc acaattaagg
     7681 gatacgtcct ggtgcaggac tgttggttat ttaactttgc aggtattatg cttcgcatca
     7741 aaaatgaagg gagaggaaaa gatgccatct ttcgatattg tctctgaagt tgatcttcag
     7801 gaagcacgta acgcggtcga taacgcgagc cgcgaagtgg agtcccgttt tgacttccgt
     7861 aacgttgaag cctcatttga gctgaacgac gccagcaaaa ccatcaaagt gttgagcgag
     7921 tccgacttcc aggtcaatca gttgctggat attctgcgtg ccaagctgct gaagcgcggc
     7981 attgaaggca gttcactgga tgtaccggaa aatatcgttc atagcggtaa aacctggttt
     8041 gtggaagcga aactgaaaca gggcattgag agcgccactc agaaaaaaat cgtcaagatg
     8101 atcaaagaca gcaaactgaa agtgcaggcg caaattcagg gcgatgagat ccgtgtgacg
     8161 ggcaaatctc gtgatgattt acaggctgtc atggcgatgg tgcgcggtgg cgatctcggt
     8221 cagccgttcc agttcaaaaa cttccgcgat taatcgcgac aacctgcgct ttgttcatgt
     8281 cagatgcggc atgaacgcct ggttcggcct acaaagtttt gtaaatcagg aacttagtag
     8341 gcctgataag cgtagcgcag caggcaattt cgcctcgctt ttatgcctgg cgaattgcct
     8401 gttcaacatc aaagcgattc gtcactttgc tgtcgatttt cacataagct gaatgttctt
     8461 cttctgcaat caacacttct ttgatgcctt cagtttccag caaacgcact tttaacgcct
     8521 cgtttgcggc aatgttcgcc gggatttcaa tgcgcaaact gctgacatac ggcggttctt
     8581 tcatggtact ggcgactgcc agccacactg cggccagcat tgcgccagcg agaaataccc
     8641 cctgaccgtc aaacatgccg tcaatccagc cgcccagcga accgccaatc gccacgccaa
     8701 gaaactggct ggtggagtaa acacccatcg ccgtaccttt gtaacctgct ggcgactctt
     8761 tactgataag tgagggcagg agggcttcca tcaaattaaa cgccacaaag aaaagctgca
     8821 cgccgaccac cagttgccag aactgcgttt gcgcgttcct caacacaatt tccgcaacca
     8881 cgatcaaccc gacgcagaag acaaagactt gcttcatttt gcgcttaact tcagcgtaga
     8941 taatgaaagg cacgaccgag ccaaaggcga ttagcattgt cgccagatag accttccagt
     9001 gttcagccgc cgggaacccc gcatcagcca gttgtccggg cagggcaaca aacgtcgaca
     9061 tcagcaaaat atgcagacac ataatgccaa agttgagttt cagcagccgc ggttccgcca
     9121 gcaccttact gaaactgcct ttcaccattc cggactcacg attaagtacg tgagtgctac
     9181 tgttgggcac aacccaaatg gtcaacgcaa tgccggtcgt tgccagaata gcgatcatcc
     9241 agaacagcgc gtgcagccca agtttgtgag tgatgatcgg gccaagcacc atcgcaatgg
     9301 caaaggtaat gccaaagctc acgccgataa acgccattgc tttggtgcgg ttttgttcgc
     9361 gcgtgagatc ggaaagcagc gccataacgg cagcggcaat cgcaccggag ccttgtagcg
     9421 cccgacccag aataattccc cagatggagt cagagagcgc agcgataacg ctaccggcgg
     9481 caaacactgc cagcccaccg acaattaatg gtttgcgacc aatgcggtcg gaaagcaggc
     9541 caaacggaat ctgaaaaacg gcctgagtca gaccataaat accaatggca ataccgatta
     9601 atgcttcgct ggcaccttgc agagccatgc cgtacgtggt cagaaccggc agaaccatga
     9661 acatgcccag catgcgcaac gagaatacgg tccctaaacc ccaggtcgcg cgccgctcac
     9721 ctggcgtcat tttataatcg ttcattacca cctctgtttt aaattcgcga ctagtgtaaa
     9781 gcggcaaacg aatggggtaa acatgcggat tttttagtaa atatttcgag agggatatgt
     9841 ttctaatgct aagaaaaaag gtgccgtagc acctttttaa tagagaggtt gtgttaccac
     9901 acagcagcca gcagcgtatg cgagtccggc accataaaat caacggacat catcaccgag
     9961 agggcagtga tggcgatgat agagaagccg aacagcttgc gcgcccagat tctgtcatca
    10021 gcaactttat aaccgcgcag agccatacct aaccaccaga cgctaaccgc cgcggcgacc
    10081 accagatatt tatatccagc gtaaccgcca agagagagca tcagcgtggc aacggcaaag
    10141 gcgatgatat acagcgtgat gtgattcttc gccaccgaaa tgccttttac cactggcaat
    10201 accggaatgt ttgccgcctg gtaatcctta aagcggaaaa tggcgatggc ataggagtga
    10261 ggcatctgcc acaggctgaa gatagccagc aggatcgctg cgccgctatc gaactcgccg
    10321 gttactgcac agtagccaat caccggcggc gcagcgccgg agagcgaacc aatcaatgtg
    10381 ccgtagacgg agtggcgttt catgtacagg ctataaacgc cgacataaac cacaaagccc
    10441 atcaccccca gccagcaggc cagcggattc gcgccaaacc acagcagcat aaagccagca
    10501 atacccagca aggtggcgta caccagcgag acagcaggag agatcaggcc tttaaccagc
    10561 acccgattct tcgtcctttc catctttctg tcgatatccc tgtcgatgta gttgttaaac
    10621 acacaacccg acgccacaac cagtgacacc ccaaccagcg tgtagataaa caggggataa
    10681 tcaatgctgc cctttgaggc cagcaggaat cccccaatca ccgagatcag gttgccaaag
    10741 atgatgcctg gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc cgctcttagt
    10801 gcatcatcat gttgtagttg aggttccaca taatccagat ggagcctaca actaggatag
    10861 cgatgattag cacggtgaag acaaacgccg tcatgttcca gccttcatct gatttggtat
    10921 tcatgtgcag gaagcacacc agatgcacca gaacctgtac cactgccatt gccaggattg
    10981 ttcccagaat tacggccgga gaggcagccc ctgtcatcac catccagaac ggaatcaccg
    11041 tcagaatgat cgacaggata aagcctgtca tgtaggtttt tacgctgcca tgggacgcgc
    11101 cgctgtgatc ggtagaatga ctcattacat cgcccccatc agataaacaa cagtgaacac
    11161 acagatccaa accacatcca ggaagtgcca gaacaggctc aggcacatga tgcgggtacg
    11221 gttagtgctg gtcaggccgc gacgagcgat ttgtaccatc agcaccgcca tccagataag
    11281 accggaagtg acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg acaggaagcc
    11341 gctgcgatcc ggacccatgc cgttaacaat caggtgatgg aattcgtaga tttccatccc
    11401 gataaatccg gcgccaaaca accaggtcaa cgccagccag gagataacct ggcttttgtt
    11461 gtttttgtac atggcgatag ccgccatgcc gtaggtgatg gagctgaaca acagcaagaa
    11521 agtttcaacc agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc cgcctgcggt
    11581 gccgttcacc agaacggcat aggtagcaaa caagatagag aacagaatgc agtcgctcat
    11641 caggtagatc caaaatccga agattttggt tccgcctgca tcgtggtgcc cgtgttcgtg
    11701 cgcgtggaca gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct gccttagtaa
    11761 tctcatcgaa atgctggttt tccagttttt cgatttctgc caccggcacg tagtaatcca
    11821 cgtcctcgtc gaagcttttc acgatccagg tgatgatcat gcctgcgaag ccaacaatcg
    11881 ccagccacca gatatgccag atcatggcga aaccgaagat ggtggagaaa gccgcaatga
    11941 cgatacctgc accgctgttt ttcggcatat gaatttcttc atagtggtca ggctttttat
    12001 acgcttcgcc tttctctttc atttcccaga atgcatcacg ctcgtgaacg tgcggcacaa
    12061 cggcaaagtt atagaacgga ggcggggaag aggttgccca ctccagcgta cggccacccc
    12121 acgggtcgcc agtcaggtca cggttctggt cgcggtcacg aatagaaacg tacatctgaa
    12181 taacgaggca gagaatacct agcgcaatca gtactgcacc gctggctgca atcatcagca
    12241 tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc atgaagccca
    12301 gcgcgtacag tggcataaag gcaacgaaga agccgatgat ccagaaccaa aacgcgcgtt
    12361 taccccaggt ttcgttcagt ttgaaaccga acgctttagg ccaccagtag gtcatccctg
    12421 cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca atcaggaaca
    12481 ggctgttatg tagaacgaag tccgcgcccg gtactgccag cagcacgcca gtcataccgc
    12541 ccaccgagaa ggtgacgata aaaccgatag tccacagcat cgcagaatgg aacacgatgc
    12601 ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg atggcgataa
    12661 tcattgtggt gataccaaag aaggcgttta cgttcgcgcc cgcacccatc gtaaagaagt
    12721 ggtgcagcca aacgatgaac gacagcacgg tgatacagac ggttgcccat accagcgagg
    12781 tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac acaccgaaaa
    12841 caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg ttgatgtaca
    12901 tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag cgatccaggg
    12961 tcaacaacgc gacggtaacc gtcagaattg ggaaggaagc aataatcagt acgttcgcgc
    13021 acagtgatgc ccaggtaaat actggcatct tgaacatggt catgcccggt gcgcgcatct
    13081 tcagaatggt aacgaagaag ttgataccgg taagcgtcgt acctataccg gatagctgga
    13141 gactccatat ccagtaatcg acaccgactc ccggactgta ctctattccc gatagcggtg
    13201 gataggccag ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg ttaaccagaa
    13261 tcacaccaac aacggtaaac cagaagctta agttgttgag gaacgggaac gcaacgtcac
    13321 gcgcgccgat ctgcagcgga accaccaagt tcatcagacc gataacgaaa ggcatcgcca
    13381 cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg tgaggtggca
    13441 ggaagcccgc ttcgcccgcc gaggcaagag cctgctggct acgcatcata atggcgtcag
    13501 caaaaccacg cagcaacatc acaatcgcca cgatgatata catgataccg aggcgtttat
    13561 ggtcgacgga ggtcagccac tctttccaca ggtaggtcca cttaccgaag taagtgatca
    13621 ggccaacgag cgccagacct cccaaaataa tgccagcaat cgtaaccatg acgataggtt
    13681 catggaacgg gactgcatca agtgataatt ttccgaacat ctttattctt cctcaacccc
    13741 tttaatgggc ggattccgcg tggctcatgt ccatgccttc cataccttcg tgtgcgctgt
    13801 gctcaccttc tggctgggtc atgtccatgc tcttaccgtg agccataaac ttgttaatta
    13861 catcggcaaa caagtttggt ttcacgttgg agaaatattc cacctggttg tattcgctag
    13921 gcgcggccag tttttcgaac gctgccatgt cagacatgga gttcggcgac tgtttcgctt
    13981 ttgcgaccca ctggtcgaat gcggcgcgat ccggtgttgc aatagctttg aacttcatgc
    14041 ctgagaagcc cgggccgctg tagctggcgg agataccgtc ataagtgccg ggttcgttgg
    14101 cgatcagatg caggcgagtc tgcataccgg ccatggcata aatctggcta cccagacgcg
    14161 gaatgaagaa ggagttcatc acggagttgg aggtcacttt gaagtacacc ggagtgttcg
    14221 ccgggaaagc gatttcattc acggtagcaa agccctgttc cgggtagatg aagaaccatt
    14281 tccagtccat ggaaaccact tcgatggtaa tgggcttctc gtcgtgtgcc agcggcttgc
    14341 taggctcaag agcgtgagtg gttttccagg tcagtaccgc aaggaagatg atgattaaga
    14401 taggtaccgt ccagaccaca gcttccactt tattggagtg tgaccagttc gggctgtact
    14461 tagcatcttt attgctcgca cggtacttcc aggcgaaacc aacagccatc aagattgcgg
    14521 gaataacgac aatcaacatc aggccaaatg ccgtcagtat cagtgaacgt tgctccagac
    14581 caatctgtcc tttgggatct aacagcgcag aattacagcc actgagcaat acagtgcctg
    14641 caaataatga caaccatccc aaacttttat tgtatttcct gagtctcatt taacgacctc
    14701 aattccacgg gatctggtgg cgtttaaaat gtgtgggcat tttacgggaa ggttacatta
    14761 ctgtaaacat gattaaattc ctgttacttg gatttggctg tcaggtcaca tatgttgcaa
    14821 aacatatcag gttgatttta agaagctgtt gcaaaagggc gttataacaa aaggggagtg
    14881 cagaagaagt ctggacgggc aattggtata atcaa
//
LOCUS       DAZGXS010000111        14807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGXS010000111 DAZGXS010000000
VERSION     DAZGXS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..582
                     /gene="espM1"
                     /locus_tag="ACHKG5_003576"
     CDS             <1..582
                     /gene="espM1"
                     /locus_tag="ACHKG5_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP0869603.1"
                     /translation="NATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFN
                     VNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKM
                     VFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANV
                     LADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(801..1857)
                     /locus_tag="ACHKG5_003577"
                     /pseudo
     CDS             complement(801..1857)
                     /locus_tag="ACHKG5_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2089..2739
                     /locus_tag="ACHKG5_003578"
     CDS             2089..2739
                     /locus_tag="ACHKG5_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0869604.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            2898..3133
                     /locus_tag="ACHKG5_003579"
                     /pseudo
     CDS             2898..3133
                     /locus_tag="ACHKG5_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3543..3644)
                     /locus_tag="ACHKG5_003580"
                     /pseudo
     CDS             complement(3543..3644)
                     /locus_tag="ACHKG5_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3868..3993
                     /locus_tag="ACHKG5_003581"
                     /pseudo
     CDS             3868..3993
                     /locus_tag="ACHKG5_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(4053..4559)
                     /gene="yciE"
                     /locus_tag="ACHKG5_003582"
     CDS             complement(4053..4559)
                     /gene="yciE"
                     /locus_tag="ACHKG5_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0869605.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4605..5105)
                     /gene="yciF"
                     /locus_tag="ACHKG5_003583"
     CDS             complement(4605..5105)
                     /gene="yciF"
                     /locus_tag="ACHKG5_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0869606.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5191..5370)
                     /gene="yciG"
                     /locus_tag="ACHKG5_003584"
     CDS             complement(5191..5370)
                     /gene="yciG"
                     /locus_tag="ACHKG5_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0869607.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5751..6557)
                     /gene="trpA"
                     /locus_tag="ACHKG5_003585"
     CDS             complement(5751..6557)
                     /gene="trpA"
                     /locus_tag="ACHKG5_003585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0869608.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(6557..7750)
                     /gene="trpB"
                     /locus_tag="ACHKG5_003586"
     CDS             complement(6557..7750)
                     /gene="trpB"
                     /locus_tag="ACHKG5_003586"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0869609.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7762..9123)
                     /gene="trpCF"
                     /locus_tag="ACHKG5_003587"
     CDS             complement(7762..9123)
                     /gene="trpCF"
                     /locus_tag="ACHKG5_003587"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0869610.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9124..10719)
                     /gene="trpD"
                     /locus_tag="ACHKG5_003588"
     CDS             complement(9124..10719)
                     /gene="trpD"
                     /locus_tag="ACHKG5_003588"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0869611.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10719..12281)
                     /gene="trpE"
                     /locus_tag="ACHKG5_003589"
     CDS             complement(10719..12281)
                     /gene="trpE"
                     /locus_tag="ACHKG5_003589"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0869612.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12303..12396)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12373..12417)
                     /gene="trpL"
                     /locus_tag="ACHKG5_003590"
     CDS             complement(12373..12417)
                     /gene="trpL"
                     /locus_tag="ACHKG5_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0869613.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12555..13436
                     /gene="rnm"
                     /locus_tag="ACHKG5_003591"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12555..13436
                     /gene="rnm"
                     /locus_tag="ACHKG5_003591"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0869614.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13433..14053
                     /gene="yciO"
                     /locus_tag="ACHKG5_003592"
     CDS             13433..14053
                     /gene="yciO"
                     /locus_tag="ACHKG5_003592"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0869615.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14081..>14807
                     /locus_tag="ACHKG5_003593"
     CDS             14081..>14807
                     /locus_tag="ACHKG5_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP0869616.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLL"
BASE COUNT         3709 a         3502 c         3687 g         3909 t
ORIGIN      
        1 aatgcgacag gtgtgtcctt cagctctttt ggtatcagtt atcataaaga taattctttc
       61 cggggcacca tccgtgggaa gaatgatgag gtcgtgaagt gttcaatggg agagcgctct
      121 atccgcttta atgttaacaa atttagcggc tgtatactgg agacggtaag caggcagagt
      181 actaaagata ttcatggatg ggtaagtgat gaacggacag tatatccgtc aagggttatc
      241 aaccaagaga ttgataattg ctgccttcaa aaaaatgcaa aaatttcttc tgaagagagg
      301 aaaatggtct tttctcttgt aagtaaggag tttgaactaa ctcttgatgt taaagcggca
      361 caaagttcta ttaatcatat aataatagga aatgcttctt ttggcaaaaa aatggatgct
      421 ctttgcgatg gtatgagccg agctgtaaaa aacagtacaa cagattacat agcaaacgtg
      481 cttgcagaca agttttatca gaaacatatc gccccgggtg ttgatattgt aaaactacga
      541 aatgaaatcc caggttatat gagtcgtgtt atacaggggt aaggttggag gaggctatgc
      601 gccagtatca ttggttttca tcctgtaaaa agatatgagt aaaatataca acaggcgtta
      661 cactatatgt atgtatccaa ataactcata gttcacctgt acagttataa atcgtaaaga
      721 aaattgcagc gcgtcgtacg cagaaacgtg ctgtgattga ctatttatag ctttgtcaca
      781 acgacaagat tcagtagtgt ttacacgggc gggagaaatg acggaataac taaatagctg
      841 cgcctaatat cgcacatttt tgccagccca tgtttgcctt cggggaatgg actgacggat
      901 tccataaaat ggcgaacttt ttcaatagtt gccacattga gcggcactga tgattacgcg
      961 ttattgtggc gtgaagtatc tgccatagct attcaaaatg attcacccat ggagattaaa
     1021 ccggctgata aatgactctg cacttcggtt tatccttcag ccagcgctgg gtttcccggc
     1081 ctttgtggat aatgtagttg tcctcgagca gcgtgatggt tttcgtccga cagtatgtag
     1141 ctttaagccg cttcagcagt ctgatgaaca gcgccgaact tttgctgttg gcgcccacac
     1201 agctgactta acctgtcccg ctgtgcagcg ctacgtccag agaatatttt tcattttgtt
     1261 ccggcgtgac ccctcgtttt tgcttcccga gcagtttcca gtccgcaccg attttgggat
     1321 taagatggat aaatacttca tcttcataaa agaccggatg ctctgtgctg cattcgtcca
     1381 gtgcgttatg gattgttgcc atctttttat ctttaggcgg ggcacggata cgtagagttg
     1441 gcgcagccct tcgccacaca agccccacag acgacaacca gcggcgaatg gttccagcat
     1501 gtaacaggca gccggttatc tcattgattt ttattgtcag cagttctgta ctccagcgtg
     1561 aataattgta gccaaagtcg cgagtgaata ttttacctgt tcacgtaata gtgtgtagat
     1621 atgcgcaaac ggccagcggc gggctcgctc ggcaggtaat gatttcagtc cctcaacacc
     1681 cgactgtgtg aactagttaa tccagcgccc aacagaggaa cgggcgcagc atagtgtgcg
     1741 ggcaacgtcg ctgacacggt cgccccggtg cagcatcagc atggctgtca gccttttatg
     1801 gatggctttc tgtattaggc gtcgttcgtc acgggaaatt ggtggtatga tcgacattgc
     1861 tcagtccggt tggtgatttg tattgatttg gcgattgatc agatcgcaca atcgggttga
     1921 gttccctcta aatgatctac tattctgcgc agctatttag tgagtatata ttttatgctt
     1981 ataaattaga ttaaaataat cctacaatta ttctgatgga gtattgctgt ggaggatggc
     2041 tttttgttaa ttatcgaaaa gtaagatata tgctttaggg ataaaattat gccattttca
     2101 tttaatttgt cttctggaaa ttacttgtca acacaagatg ttgaagtttt acaacgtgcg
     2161 accagagatc atcaaatgga aagactcaca ataggagaaa gatcgttttc tgttcgctat
     2221 cagagtgcta tggacgcttt tattgtagat cctgttcaag gggaactata ttcgggttta
     2281 agccatacag aactagccga tatcattaga ttggctgatt ctgttgaaaa tcaattgaat
     2341 ggaggcaatt catttcttga tgtattcagt acatatatgg ggcaggttat ttctgaattt
     2401 atgcatagta atgataacag aattgaattg ttacagcggc gattacattc atgttcattt
     2461 ttagttaata ttgaagaaat gtcttacata gatgaagcat tacagtgccc gattacgctg
     2521 gcaattcctc aacgaggtgt ttttttaaga aatgctgaag gttccagagt atgtagttta
     2581 tatgatgaaa tggctctttc tcgtataatt aatgatggga tgcatcaccc actaagcaga
     2641 gagccaataa cattatcaat gcttgtggcc agagagcagt gtgagtttga ttgcagtatc
     2701 ggtcacttta cggtgaggag tgattgttat tcagtgtaga ctcggtacac gattttgtgt
     2761 gaagagactc ttattaaaag ttagcatcaa agcggtcatc aaacttgata ataaagtgac
     2821 tcattgctat tcgctggtcc ttaagggatg tatcgtgaac tgaatagcaa gtagccaccg
     2881 tcctgatttc aacgcattat caggcgcttc tgaagcggca tacctgcatg acaggatgag
     2941 tgcaaagggg cgctgttacg ataatgctag ggcggaaagt ttcttccact cactaaaagt
     3001 cgaatgcatt tacggctaat attttatgag tcgggaaata atgcagacgg ctgtgtttaa
     3061 ttagatctag tgtgattaca atcggtggtg ccgacacagt acttgtggct gtctcagcac
     3121 ggaactgttt tgaaaaacag aaccttgatt agggctgtgt ccacattacg taggtaggat
     3181 caccccacag gcaccacatg tcatgcatgg attaggattg aatgataaat cgtaaagaaa
     3241 actgcaacac ctcgcgtgta gcacctggtt agcgaacttt cgaaagtacg agtattgtat
     3301 gatttccagc agttatcaat tttacgtatt aatcagtgaa tcaacaactc gtcagcagac
     3361 tctctttgct gttgaatttt ttggaacgtg tatagggcag aaatttttgt tttgggacat
     3421 ggttgggacg ccgtagtaca tacgaactct gccgaataac gtatgatatt attttttaat
     3481 ctcttggaat acattataga aacagaatgc tcttgggcga tttttagtga ttgtttaaat
     3541 agccctgtca tgcagttaaa gtggcgggca tcctcttcaa ggatggtgat cctcaaccgc
     3601 acccatttag gatgcgacta ttgtggtaga acaatgtaaa ccataattaa tctgcgtgcg
     3661 gatacatgct tcatagatag gatattctgg tttgggttgt gaatgtgttg gtcatcgagt
     3721 ggacaataag gtaagaggag taactcctgc aaaacaaaaa aagagttcaa caaaatcaat
     3781 cagttaaaaa cacgagagat ctgtataaaa aagcatcgta aattctgtat ttttccttta
     3841 aatataatat gttattgagt tttgtaaaat ctaccagtgt ggtacatgga tatcgatacc
     3901 accgccaatt ataaactggg cggtgcacag caacatgata gcgtacgcct cgatccgtgg
     3961 gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac cccgtaatat
     4021 acggggtcaa taaggacatg gcataaaggg tattatttct tcgcttctac gccatcagtt
     4081 tcagagcgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc
     4141 atttgctttt cctcatttaa aatcgcttcg atgatcggaa ttgaggctgt atcaccggca
     4201 ttttttgctg ctgctaatag agaggtgtaa caggcgattt caaattgctc gaagacatat
     4261 ccgctaatag agcctttgac tatttcatca gaagggaata taccaccgat tgactgccca
     4321 agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc attacgatca
     4381 agaatagttt ccagttgaac aatctggttt ttggtttcac taagatgttg ttcaatacga
     4441 gcacgtagtt caggataatt atctatacgg ctggccatgg attcaagcat agattcggct
     4501 tgcttttcca ttgcatgggc gtcacgtaac cagtcatgat aatgttcaat acgattcatg
     4561 aaaatgtcct ctttataaaa tgaccaaaag ttaaagttca tatttcaggc tttattttcg
     4621 gctttcttat ttacgttatt aagggccaga tcagtcagtt tgatgtcggt ggccttttct
     4681 tcttccaggg tctctttcag aagcttcgct gctttacggt aacctaattg ttcagccagc
     4741 gtcgctaatg tcccgtaact ggcaatctca taatgctcga ctttctgtgc tgcggcaatc
     4801 agtgcggcat cacgcacttc gtttttctcg gtactttcga tgacctcatt agcttcttca
     4861 ataagacctt ccattgccac acatttcatg cgcttaattt tcagattcga ttctgattcc
     4921 acaacttgat caatacgttc aatctgtcca tgtgtttcct cgaggtgcgc atgaaaagcc
     4981 tgacttaatt tttcgtttga tgttgctctt gcgagttttg ccagtgcccg ggttaattgt
     5041 ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata catcttcaat ggtcttcata
     5101 ttcatatttt tctccagtga aatcactgcg agccatacgg cccgcagatg caattatctt
     5161 tatcagctga tattaataat taacggtgaa tcaggatttt cctgatttat taccaccgct
     5221 ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc
     5281 gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc ttctcacggt cttcggcgaa
     5341 atttcctgaa ccaccacgat gttcggccat gttatttctc ccgttgcgtt gcattgtttc
     5401 attaatatga gtgttgtgtg tcgatactca ttaaaattag tcgctaatga ggattagtca
     5461 aattaagcgt aacgagaaga aagtaggaaa atatattatg acgaacgttc ttgatatatt
     5521 aacaattgcc tgcggaataa gtgacttaga gaaaattatt gattttactg gtgttatgtt
     5581 gcgggattaa attgtttgat tatattactg ttgggcggaa aaatgacgta agttgacgtt
     5641 cgacaggggt aagcgaaacg gtaaaaagat aaatattaaa tgaatttagg atttttccgg
     5701 cttcgttaaa gaaagttaaa atgccgccag cggaactggc ggctgtggga ttaactgcgc
     5761 gtcgccgctt tcatcggttg tacaaaagct ttcagtgccg ccagcatttt ctttggctca
     5821 ttaatatgtt gctcgatgat tttaacaatc gcagaaccag aaatcgcgcc cgcagctcct
     5881 gcatcaatcg ctgcttttac ctgatccggg gcggaaatac caaatccctg caatggaggt
     5941 gccgcgttgt actctttcag cttcgcaacc agatgattga ggggtaacgc ggcgcggttt
     6001 tctgcgccgg tcacgcccgc tcgtgacagc aaatatgtgt aaccacgacc gtaagaggct
     6061 atctggcgca gcaggtcgtc atcggcattt ggcgggcaga tgaagatagg tgcgacatta
     6121 tgacgcaacg cggcctggcg gaagggcgcg gactcttcaa ctggcacatc ggcaaccagc
     6181 accgaatcga cgccgacttt ctcgcactgg gcataaaact catcaatgcc tttgttaaac
     6241 accaggttgg catacatcag caggccaatg ggaatggtcg ggtgtttctg gcgaatgagt
     6301 gccagcatct caaagcactg cgccggggtc acacctgccg caaaggcacg cagtgtggcg
     6361 ttttgaatcg tcgggccatc tgccagtggg tcggagaagg ggatgcctaa ctccagcgtg
     6421 tcagcaccgg cttcaattag cgtatcgata attttcagcg actgctcaat gcccggatca
     6481 ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct ccttcaactg ggcaaacaga
     6541 gattcgtagc gttccatcag atttcccctc gtgctttcaa aatatcgtga acggtgaaga
     6601 tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag ctgctctttt tccgggtttt
     6661 cgcgcatcat tttcagcgca tgggccaggg cgtgggagga ttccagcgcc gggataatcc
     6721 cttcgtgcag gcacagcgtt ttgaaggctt ccagtgcttc atcatcggta attgacacat
     6781 aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg gccgacggac ggaaaatcga
     6841 gtccggcgga gatggagtaa gattcttcaa tctgcccgtc ttcggtttgc atcatcggcg
     6901 ctttcatacc gaaatagata cccacgcgac catgttttag cggtgcgccg tgctcgccag
     6961 tttcgatacc gtgaccacca ggctccacac caatcaggcc gacgttggtt tcgttgatga
     7021 aatctgcaaa catgccgatg gcattcgaac cgccgccaac acaggcgata acggcatccg
     7081 gcaggcgacc ttctctttcc agaatctgtg ctttggtttc ttcgccaatc atccgctgaa
     7141 actcacggac aatggtcgga taaggatgtg ggccagctgc ggtgcccagc atatagtgcg
     7201 cggtttcata actgccggac cagtcgcgca gcgcctcgtt acaggcatct ttcagcgtcg
     7261 cggaaccgct atgcaccggg atcacttccg cacccattaa acgcatacga aaaacgttag
     7321 gcgactggcg ttcaacgtct ttggccccca tataaatacg gcatttcagg ccgagcaggg
     7381 cgctggcaag ggccgacgcc acgccatgct gaccggcccc ggtttcggcg atgatttcgg
     7441 ttttacccat ccgcttcgcc agtaaagcct gaccgagcac ctggttagtt ttatgcgcgc
     7501 cgccgtgcag caaatcttcg cgcttcagat acagcgtggt gttcgtgcca gccgtaatgt
     7561 tctggcattt ggtcagcgcg gttggacgcc cggcatagtt tttcagcagg tcgttgaact
     7621 gagcctgaaa ttcaggatct ttttgcgcgc tgacaaaagc ttcttccagc tggcgcagag
     7681 caggcatcag gatttgtggc acgtacatgc cgccaaactc accaaaatag gggttaagta
     7741 atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc tggaaaaccg aggccaaaag
     7801 acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa ttaaaatcaa gtccggcgca
     7861 gccggtttgt gccgcttcca cgcagttatc tgcgcctaag ccccccgcca gcagaacgtt
     7921 gccaagcgat tgaccattta atagtgacca gtcgaaacgt tgcccgcttc caccctggcc
     7981 gttgtctaaa acatatttat caacgtgctg aagctcgcgg gcgggcaggg tttcaccgac
     8041 gcttaatgct ttccagatgg caacgtgtgc tggcagagct tcacgcagcg tatcgatata
     8101 cagctgatct tcattaccat gcagttgcac tgccaccagt gataacacct tagctttgtc
     8161 cagcacatcg gcaatatcgt gattgcggaa cacgccaaca tactgcaacg gtgccgcagc
     8221 catcacttcc tgcgcctgtt caacgttgac gcaacgcggt gatgtcgcaa caaaaatcaa
     8281 cccaccgtaa atcgcgcccg cgtcgtacgc tgctttagca tcttgcccac gcgtcaggcc
     8341 acatacttta ttctcaccca gcaacacccg acgcacggcg gcgtgcaaat cgtcatgggc
     8401 catcaacgcc gaaccaatta gaaaaccgtc agcgaagtgg cttaactcgc gcacctgagc
     8461 gtaagtattg atgcctgatt cgctgattac cgtcacgttg tgccccagtt tcggcgcaag
     8521 ctcgcgggta cggttgagat caatcgacaa atcacgcaga tcgcggttgt tgatgccaac
     8581 gacctttgcc cccaatgcaa tggcgcgctc cagttcctct tcattactga cttcggtcag
     8641 cacacccatc ttcagactgt gggcgacggc ggcaagctgg cgatattgtt cgtcatccag
     8701 tactgaaagc attaataagc aggcatcggc ctggtaatag cgcgccagat agatctggta
     8761 agggtcgata atgaagtctt tacataaaat cggctgcggg gcgatttggc tgacgatggg
     8821 gaggaaatca aagctcccct gaaaatattt ctcatcagtc agcaccgaaa ttgccgaagc
     8881 gtaatgttta taaatggcgg caatgcgtgc tggatcgaaa tcatcacaga tcacgccttt
     8941 tgacggcgac gctttcttgc actccagaat aaacgccgtg cgcgcaccct gtagcgcatc
     9001 ataaaaatgt cgcgtgctcg gctgaacctc attctgaaaa ctggccagcg gttgctgctg
     9061 tttgcgggct tctacccaaa tcgccttgtc tgcgacgatt ttcgctaaaa cggtttgcat
     9121 catttaccct cgtgccgcca gtgcggtgac tctgtcgtaa gcggaaccac tgcgcagtac
     9181 ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg ccatgcaggc gcattaacat
     9241 ggcgacattc gccgcgacgg ctgcttcatg ggcggcgtcg cctttacctt gtaacaagcg
     9301 tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc agttgctcct ggtggtaggg
     9361 agtcaggcca aaatcttcag cggtcaattg atagctctta atttcgccgt catgcagctc
     9421 ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc ccgccgctgt gcaccaccgc
     9481 cgcgcgttga taccccagca cgcgtaaggt ttcggcaatc ggcagcacca gttccggact
     9541 ataaacacca attaacgcca gcggcggatg cgccgggtta atcaatggcc ccagcacatt
     9601 gaacagggtg cgggttttta gttgctggcg aaccggcatt gcatggcgaa atccggtgtg
     9661 atatttcggt gcaaagagga aacatacacc taactcatcc agcgcctggc gcgatttatc
     9721 ggcgttcata tcaagattaa taccgaacgc cgccagcaga tccgacgagc cagatttact
     9781 ggagacgcta cggttgccgt gtttcgccac tttcagccca caggccgcgg cgacaaacgc
     9841 actggcggtg gaaatattga tgctgttgct gccgtcaccg ccggtgccga cgatatcggc
     9901 aaacagataa tccggacgcg ggaacggcgc ggcgttttcc agtagcgcgg ttgctgctcc
     9961 ggcgatctcg ttcgggtgct cgccgcgaat tttcatgctc accagcgccg ccgccaggtg
    10021 ttccggtttc agttcaccac gtaccaccgc tgaaaatagc tgatgacttt cctgctggct
    10081 aagggtctgc gcctgataca gtttttccag aatcggttgc agcgtgttgg ttggctctag
    10141 tttctgctgc gcccaggcca gcgtttgttc cagcaggcga gcgccctggg tggtgagaat
    10201 ggattccggg tggaactgga atccgcaaac gcgatctgca tcgtgacgca ccgccatcac
    10261 catgccatta aaatgggcgt tgatggttaa accggccgga atattactgc caaccagcga
    10321 gtgataacgc gccaccggca gcgggtttgt taatccggca aacatcgcct gaccgtcatg
    10381 ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc tgaccgacat agcccccgta
    10441 agcttcgaca atcgcctgat gaccaaggca aatgccaata ataggcagct taccgcgcag
    10501 gcgggtaagc agttcaggca tacaaccagc ttcgctcggc acaccgggtc caggagaaag
    10561 catcagcacc ggattgctca tcgtcgcaag gcgttcaatt aaggtctgcg ccggaatatg
    10621 gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac tgatctgcca ggttgtaagt
    10681 aaaagagtcg atattatcga gcagcagaat gtcagccatt agaacgtctc ctgtgcatga
    10741 tgcgcggtgg caatagcgcg cagtacagcg cgggctttat tacgggtttc gtcggcttcc
    10801 gactgcggaa cagaatcaag gactacgcca gcaccggctt gcacggtagc gataccgttt
    10861 tccaccagcg ccgagcggat cacaatgcag gtgtcgagat cgccgtgcgc ggtgaaataa
    10921 cctaccgcgc cgccgtagct gccgcggcga cgaccttcag cctcggcaat taactgcata
    10981 gcgcgtactt tcggcgcacc gcttaacgtc cccatattca tacaggcgcg gtaagcgtgc
    11041 agggcgtcga gatcgtggcg cagctcaccg accacgcggg agaccaggtg catcacgtaa
    11101 gagtaacggt caactttggt gaggtcggcg acgtagcggc tgccgggggt gcaaatgcgt
    11161 gccaggtcat tacgggcgag atccaccaac atcagatgtt cagaaagctc tttatgatcg
    11221 gtacgcattt ccagttcaat acggctttca aggtccctgt cgagcgagcc atcggcgcga
    11281 cgaccgcgtg gacgtgttcc ggcaatcggg tagatctcaa tctggcggct ggtggcgtca
    11341 tacttgagcg aactttccgg cgacgcgcca aacagggtga aatcattatc ctgcataaaa
    11401 aacatgtacg ggctgggatt actctttttc agcacgtaat aggccgccag cggtgacggg
    11461 cagggcagag agaaacggcg agatggcacc acctggaaaa tttctccggc gcgaatcgct
    11521 ttttgcaaca aacgcactac gccaccgaac tcttcatcgc tctggttaca ttcacaacgc
    11581 atatgcggca cggaaaccac cggcagcggt ggcgcggttt cggtcagttg ctggcgaaga
    11641 tcgttcaggc gagcagtgag acgttgtttt tcttcttcat tcggagcaaa caggctggcc
    11701 tgaatgcggg tgcttttttt ctgatggtca atcaccatca gagtttcagc gagataaaaa
    11761 cagaaatcag ggcagttatt ttccgctgac agttgcggta aatcttcaaa ccccgccaca
    11821 aggtcataag agaacaggcc gccgaagaac atggcttctc gttcttcctt cggtacattc
    11881 aacagattct gcaataagcg gaaagcgtca aaaaccgaaa gggagcataa gcgagcgtct
    11941 tcatccagca gtggactaac aggggggaag cgcagcacac ggcagtttgg taattgttca
    12001 ttttccacac ccgcaggcag ggcgttatcc agtagtgtca gcagggcttc gccgttgccg
    12061 gaaagggact ggattgtgac agtgtcacct aaagctgtaa tgcgcagcgc actgtctacc
    12121 agcagcaggc tttttaaatc atctttgctg tcgatatctg cggattccag cagcagcgtt
    12181 gccggacgat ccccacacaa ttggtgaaaa agcgcggtgg gattgtcgcg ataagcgcct
    12241 tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca ttgttattct ctaattttgt
    12301 tcaaaaaaaa gcccgctcat taggcgggct gggtatctga ttgctttacg catggtgaat
    12361 acactgcccg tttcaggaag tgcgccacca acctttcagt acgaaaattg ctttcattgt
    12421 cgataccctt tttacgtgaa cttgcgtact agttaactag tttgatgatt gattgtcaac
    12481 agctcatttc agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag
    12541 aacgggagtg cgccttgagc gacacgaatt acgcagtgat ttacgacctg cacagccata
    12601 ccacagcttc cgatggctgc ctgacgccag aagcattggt gcaccgtgca gtcgagatgc
    12661 gcgtcggcac cctggcgatc accgaccatg acaccacagc tgcaatcgcg ccagcaagag
    12721 aggaaatttc acgttcggga ctggcgctga atcttattcc cggcgtggaa atttccacgg
    12781 tctgggaaaa tcatgaaatt catattgtcg ggctgaatat tgatattact catccgctaa
    12841 tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca gcgggcgcag caaattgccg
    12901 aaagacttga aaaagcgcat atacctgggg cgctggaagg cgcacaacga ctggcgcagg
    12961 gcggcgcagt gacgcgcggt cattttgcgc gtttccttgt tgagtgcggc aaagccagtt
    13021 caatggcgga tgtctttaaa aagtatctgg cgcgcgggaa aaccggatac gttccgccac
    13081 agtggtgtac aatagaacaa gctattgatg tcattcatca ttctggcggt aaggcggtgc
    13141 tagctcatcc tgggcggtac aatcttagtg ctaaatggct gaaaagattg gttgcgcatt
    13201 ttgccgaaca cgacggcgac gcgatggaag tcgcgcagtg ccagcaatcg cccaatgaac
    13261 gtacccagct ggcgacgctt gcccgccagc accatttatg ggcatcacaa ggatctgatt
    13321 ttcatcagcc atgcccgtgg atcgaactgg gtcgtaaact ctggctgccc gcaggcgttg
    13381 aaggcgtctg gcagctatgg gaacagccgc agaacaccac agagagggaa ttatgagcca
    13441 gtttttttat attcatcctg ataacccgca gcaacgtcta atcaaccagg cggtggagat
    13501 cgtgcgtaaa ggcggggtga ttgtttatcc aactgattcc ggctatgcgc tcggctgtaa
    13561 aattgaagat aaaaacgcca tggagcgtat ttgtcgtatt cgccagctgc cggacggtca
    13621 caactttacc ctgatgtgtc gcgatctttc tgaactctcg acctattcat ttgtcgataa
    13681 cgttgcgttt cgtttaatga aaaacaacac gccgggcaac tataccttca tcctgaaggg
    13741 gacgaaggag gtgccacgcc gtctgttgca ggaaaaacgc aaaaccatcg gtatgcgtgt
    13801 gccttctaac ccgattgcgc aggcgttgct tgaagcactg ggcgaaccga tgctttccac
    13861 ttcgctaatg ctgccaggca gcgaatttac cgaatccgat cccgaagaaa tcaaagaccg
    13921 tctggaaaaa caggtcgatt tgattattca cggcggctat ctcgggcaga aaccaacaac
    13981 ggttattgat cttaccgatg atacgccggt cgtggtgcgt gaaggcgtag gtgatgtgaa
    14041 gcctttctta taacaaattg cgttccacgg atggaagact atggcaggga agtttcgctg
    14101 cattttgctg ttgatagttg ggcttttttt ctcttcgtta agttatgcga aaaacacgga
    14161 gatgccttct tatgaagaag ggatctcgct ctttgatgtt gaagccactc tgcaaccgga
    14221 tggggtgctc gacatcaaag aaaatattca ttttcaggcg cgaaatcagc agattaagca
    14281 cggattttat cgtgatttac cacgactatg gatgcagcct gatggggacg ctgcactgct
    14341 gaactatcat attgttggcg tcacccgtga tggtattcct gaaccctggc atcttgactg
    14401 gcatatcggg ttaatgagta ttgtcgtggg cgataaacaa cgtttcttgc ctcaaggcga
    14461 ctatcattat caaattcatt atcaggttaa aaatgctttc ctgcgtgagg gagattcaga
    14521 tctgttaatc tggaacgtga ctggtaacca ctggccgttt gaaatctata agacccgatt
    14581 ttcactcaag ttccctgata tcgcgggtaa tccatttagc gaaatcgatc tctttactgg
    14641 agaagagggc gacacatatc gaaatggccg catccttgag gacggaagaa ttgaatccag
    14701 cgatccgttt tatcgtgaag atttcacggt actctaccgt tggcctcact cgttacttag
    14761 caatgccccg gctccacaaa cgacgaatat tttcagccat cttcttt
//
LOCUS       DAZGXS010000112        14487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGXS010000112 DAZGXS010000000
VERSION     DAZGXS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..438
                     /locus_tag="ACHKG5_003594"
     CDS             <1..438
                     /locus_tag="ACHKG5_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFP0869617.1"
                     /translation="NNNFFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEI
                     EDNLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKS
                     RHEMLPVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            661..1158
                     /gene="ubiC"
                     /locus_tag="ACHKG5_003595"
     CDS             661..1158
                     /gene="ubiC"
                     /locus_tag="ACHKG5_003595"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0869618.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1171..2043
                     /gene="ubiA"
                     /locus_tag="ACHKG5_003596"
     CDS             1171..2043
                     /gene="ubiA"
                     /locus_tag="ACHKG5_003596"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0869619.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2198..4621)
                     /gene="plsB"
                     /locus_tag="ACHKG5_003597"
     CDS             complement(2198..4621)
                     /gene="plsB"
                     /locus_tag="ACHKG5_003597"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0869620.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4792..5160
                     /gene="dgkA"
                     /locus_tag="ACHKG5_003598"
     CDS             4792..5160
                     /gene="dgkA"
                     /locus_tag="ACHKG5_003598"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0869621.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5270..5878
                     /gene="lexA"
                     /locus_tag="ACHKG5_003599"
     CDS             5270..5878
                     /gene="lexA"
                     /locus_tag="ACHKG5_003599"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0869622.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5951..7276
                     /gene="dinF"
                     /locus_tag="ACHKG5_003600"
     CDS             5951..7276
                     /gene="dinF"
                     /locus_tag="ACHKG5_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0869623.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7392..7601
                     /gene="yjbJ"
                     /locus_tag="ACHKG5_003601"
     CDS             7392..7601
                     /gene="yjbJ"
                     /locus_tag="ACHKG5_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0869624.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7643..8158)
                     /gene="zur"
                     /locus_tag="ACHKG5_003602"
     CDS             complement(7643..8158)
                     /gene="zur"
                     /locus_tag="ACHKG5_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0869625.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8477..9433
                     /locus_tag="ACHKG5_003603"
     CDS             8477..9433
                     /locus_tag="ACHKG5_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP0869626.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSTM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYN
                     DNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKM
                     RDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
     gene            9795..9899
                     /locus_tag="ACHKG5_003604"
                     /pseudo
     CDS             9795..9899
                     /locus_tag="ACHKG5_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            10046..10579
                     /locus_tag="ACHKG5_003605"
     CDS             10046..10579
                     /locus_tag="ACHKG5_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869627.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            10776..10949
                     /locus_tag="ACHKG5_003606"
     CDS             10776..10949
                     /locus_tag="ACHKG5_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869628.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(10997..11278)
                     /locus_tag="ACHKG5_003607"
     CDS             complement(10997..11278)
                     /locus_tag="ACHKG5_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFP0869629.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(11315..11665)
                     /locus_tag="ACHKG5_003608"
                     /pseudo
     CDS             complement(11315..11665)
                     /locus_tag="ACHKG5_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(11623..11820)
                     /locus_tag="ACHKG5_003609"
     CDS             complement(11623..11820)
                     /locus_tag="ACHKG5_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869630.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            complement(11967..12440)
                     /locus_tag="ACHKG5_003610"
                     /pseudo
     CDS             complement(11967..12440)
                     /locus_tag="ACHKG5_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(12437..12787)
                     /locus_tag="ACHKG5_003611"
     CDS             complement(12437..12787)
                     /locus_tag="ACHKG5_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFP0869631.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            complement(12778..13314)
                     /gene="yfdR"
                     /locus_tag="ACHKG5_003612"
     CDS             complement(12778..13314)
                     /gene="yfdR"
                     /locus_tag="ACHKG5_003612"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0869632.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            complement(13442..13858)
                     /locus_tag="ACHKG5_003613"
                     /pseudo
     CDS             complement(13442..13858)
                     /locus_tag="ACHKG5_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            13846..14487
                     /locus_tag="ACHKG5_003614"
                     /pseudo
     CDS             13846..14487
                     /locus_tag="ACHKG5_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
BASE COUNT         3614 a         3312 c         3622 g         3939 t
ORIGIN      
        1 aataacaatt tttttatcca acttatcgat caggctctac gatcaacgaa tgatacgatc
       61 aaagaaaatg cctggcatct ttataaagag tggattcgta gtgatgatgt ttcaccttta
      121 tttatagaaa ttgaagataa tttaagaacc tttaacacga atgaattaac acgaaacgat
      181 aatatcttta tcttgttctc ctctgtcgat gatgggccag ttatggtggt aagctcccag
      241 cgcttacatg atatgttgaa tcctacaaaa gataccaatt ggaattccac gtatatctat
      301 aaatccagac atgagatgtt gcctgttaat cttactccgg aaacactttt cggctccaaa
      361 tcttatgata aacatgcgct tttccccatt tttactgcga gttggcgagc taatcgtata
      421 aagaataaag gtatttaagt aaaggaaaac atcaccgctc ttggcatcat gggcggtgat
      481 gcccctatgg ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt
      541 taccacttca tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca
      601 tctcccgtaa acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc
      661 atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct attgtaaaga gatccctgcc
      721 ctggagccgc aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa
      781 cagcagggaa aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa
      841 atccccgaag aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg
      901 ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta
      961 agcgggccgg agctggcgtt acaaaaattg ggtaaaacgc cgttaggacg ctatctgttc
     1021 acatcatcga cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg
     1081 cgacgttccc gcttgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg
     1141 gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg
     1201 ctggcgtttc atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg
     1261 ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg
     1321 gtgtttgtcg cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct
     1381 gaccgcaagt ttgatggtca tgttaagcgc acggcgaacc gaccacttcc cagcggcgcg
     1441 gtaacagaga aagaggcgcg cgcgctgttt gtagtgctgg tactgatttc gtttttactg
     1501 gtgctgacgc tgaatacgat gaccattctg ttgtcgattg ccgcgctagc gctggcgtgg
     1561 gtgtacccgt ttatgaagcg gtatacccat ctaccgcaag tggtgctggg cgcggcgttt
     1621 ggctggtcga ttccaatggc ttttgctgct gtgagtgagt cggtgccatt gagttgctgg
     1681 ttaatgttcc tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcgatggtt
     1741 gaccgtgatg atgatgtgaa gattggcatt aaatccaccg caatcctgtt cggccaatac
     1801 gataaattga ttattggtat tttgcagatt ggcgtactgg cgctgatggc gattatcggt
     1861 gagttaaatg gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt
     1921 gtttatcaac aaaaactgat tgccaaccgc gagcgcgaag cctgctttaa agcatttatg
     1981 aataataact atgttggtct ggtactgttt ttagggctgg caatgagtta ctggcatttc
     2041 tgatgatgta aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc
     2101 gcttctcagg cctacacaac agatcgcaat ttattgaatt tgcagattat ggaaggccgg
     2161 ataaggcgtt tgcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac
     2221 tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca
     2281 tcgtttctgc cggttcggca tcgccgctat cgctgatata cccttcatca cgcagcgtaa
     2341 gcaccagaga actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca
     2401 cggagagacg ttgcgcgacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg
     2461 ggttggcgct caacagccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg
     2521 cggcgagcag ctgtagcgtg cgagaatgca ccgggttgat atgcaactca tcatcttgca
     2581 gggtaatcag cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact
     2641 cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt
     2701 gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg
     2761 aaggtagcac caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct
     2821 cgcgcggcag aatgatgatg tcgccgatag tgtctttctc aacttcaaac ttgttcattt
     2881 gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt
     2941 aaggcacgtt gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg
     3001 tgagtgagcg ctgacgtgat gccagtagcg cagtacagca caggttcatg gcgtttgccg
     3061 cgcctgcgtt gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta
     3121 accatgccgg acgcactgct tcgatgggat cgatagattc acgccagtct ggtacgtgct
     3181 ggttaaggta ggtcatcaac ggcattggtt caccgaaatt gacgtaaccc tggccgagat
     3241 tacgcagctt gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc
     3301 cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg
     3361 gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg
     3421 taccggtttt cggatccagc aaacgccccg tacgggaacg accgccttct acgaagtact
     3481 cgacggaata accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataga
     3541 gcttattgcc tttaaaagta cggcgaataa agaacgcacc cagacggcgg aaaatcggcc
     3601 cggcaggcca gaaattcagg ttgatcccgg cggcgatatg cggcggcact agcccctggt
     3661 ggtacagcac gtaagaaagc agcaggtagt ccatgtgact gcggtggcaa ggcacatata
     3721 ccagctcatg accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc
     3781 cctggtaaag gcggttccag gtaaagccca gaatacggtc agtcaggcgg atcatctcgt
     3841 aagagaaatt cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat
     3901 gggagatttt tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga
     3961 gcagcttatt aaacagatcc tgacgagcag gcagacgtgg tcctacggca gccagacgtt
     4021 gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg cgcgatagtt ttatccgtgc
     4081 cgtgttcatc cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc
     4141 gaccgagcca cagtacagcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt
     4201 tcacttcgcc tttttcacgc cccggcgcgc gaccaaacat caccgacact ggcaccatct
     4261 gcacatccag atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag
     4321 actcttcttt cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc
     4381 gcggcagtag cgtgccgtcg atttccagcg gctctaacgg gtcaggcaag tcatgtgcca
     4441 gacactgggc gcgcaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa
     4501 ttggacgaga ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt
     4561 ttaccaggat gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca
     4621 taaacgatgt aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca
     4681 tgggaaattc tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg
     4741 gatttaacgg tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat
     4801 aataccactg gattcacccg aataatcaaa gctgctggct attcctggaa aggtttacgc
     4861 gctgcatgga tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg
     4921 gtcatcgcct gctggctgga tgtggacgcg attacccgcg tgctgcttat cagctccgtg
     4981 atgctggtga tgattgtgga aatcctcaat agcgccatcg aagcagtggt tgaccgaatt
     5041 ggctctgaat accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg
     5101 attgcgatta tcgtcgccgt gattacctgg tgcatcctgt tatggtcgca ttttggataa
     5161 cccttccaga attcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct
     5221 tttgctgtat atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt
     5281 aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat
     5341 gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga
     5401 agaacatctg aaggcgctgg cacgcaaagg cgtaattgaa attgtttccg gcgcatcacg
     5461 cgggattcgt ctgttgcagg aagaggaaga agggttgcca ctggtaggtc gtgtggctgc
     5521 cggtgaaccg cttctggcgc aacagcatat tgaaggtcat tatcaggtcg atccttcctt
     5581 gttcaagccg aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg
     5641 cattatggat ggcgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt
     5701 cgttgtcgca cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggcaataa
     5761 agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca
     5821 gagcttcacc attgaagggc tggcggttgg cgttattcgc aacggcgact ggctgtaaca
     5881 tatctctgag accgcgatgc cgcttggcgt cgcggtttgt ttttcatctc tcttcatcag
     5941 gcttgtctgc atggcattcc tcacttcatc tgataaagca ctttggcatc tcgccttacc
     6001 catgattttc tccaatatca ctgttccgtt gctgggactg gtcgatacgg cggttattgg
     6061 tcatcttgat agtccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt
     6121 tctctttatg ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta
     6181 tggtgccaaa aatcctcagg cattagcccg tgcgctggtg caacctttgc tgttggcgtt
     6241 gggggctggg gcgttaattg cgctgctgcg tacgccgatt atcgatctgg cgctgcatat
     6301 tgttggcggt agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt
     6361 aagcgcaccg gcgtcgctgg cgaatctggt attacttggt tggttactcg gcgtgcaata
     6421 tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt
     6481 ctggctggtg atgggactgc atatgaacgt gcggggcgcg gcgctggcga cggttattgc
     6541 ggaatatgca acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg
     6601 aatctccggc gaaatgctga aaacttcctg gcgaggaaac ttccgtcgct tgctggcact
     6661 taaccgcgat atcatgctgc gctcgctgtt gttgcaactc tgtttcggcg cgatcaccgt
     6721 acttggcgcg cgactgggga gtgacattat cgctgttaac gcggttctga tgacgctact
     6781 cacctttacc gcctatgcgc tggacggttt tgcctacgcg gttgaagcac attccggtca
     6841 ggcatacggt gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag catgccgcca
     6901 gtcggggatc gtagcgttac tgttttcggt ggtttatttg ctggcagggg aacacatcat
     6961 tgcgttactg gcgtcgttaa cccagattca gcagctggcc gaccgctatc ttatctggca
     7021 ggtgattttg ccgttggtcg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc
     7081 aacgcgtgct gctgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac
     7141 gctccttacg ctgccgtggc tgggtaatca tggtttgtgg ttggcgttaa ccgtctttct
     7201 ggcgttgcgc gggctttctc tggcagctat ctggcggcgt cactggcgca acggtacctg
     7261 gtttgccgca acgtgacggt taaaaattct gaataaataa tcctaagcca aattgctgac
     7321 tacacttaat ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg
     7381 aggtcttgat tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag
     7441 tgaaagagca atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg
     7501 atcaactggt cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag
     7561 aggtcgtgga ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac
     7621 gtgtacaagg atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat
     7681 gctggcactg ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat
     7741 gtgcttcaat cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcatga
     7801 tgtcttccac gccttcagca cattcttctt tcaccgcgcc gcagcgatcg caaataaaca
     7861 tagctgacgt atgggtgggc tggtcgaaca gatggcagag cacataactg ttggtggatt
     7921 ccaccttatg cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg
     7981 gcttggcttg cggttcagct tcgcgcagca aatcaagcag atcataagcg ctgatagcgc
     8041 catcctgcag actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac
     8101 gctgcgcgca gattttgtca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg
     8161 ggcacctcaa agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg
     8221 agaaccctta ttgttgccgt aatgttgctt ttctgttttg taggtaagat gttgtgttgc
     8281 ctggcagtac cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc
     8341 aagttaactc gctatttatt taaccgtggc taatcgtata cccgaatttc agaaaattat
     8401 gagcgatttt tgatagttac cggttccaaa aacacttata aggaggacac ggtacgacaa
     8461 aaaaggtaga tgaatcatgt taaagataat acccggtgca acaggatact ttaataaaac
     8521 actcaattca aatcaattcg ataatgaaga tgcaatcaaa ggtaaagtaa atagtatata
     8581 tggtaaatct attgattatt caacgatgcg tcatcgggat ataattattg caaaaataga
     8641 tttatttatt caaagaatca ctcataattt atggactgca cgtgaaaaaa atgtgacgtt
     8701 aattaagcaa ataaacgatc tcaaaatgtg tgtcaatgag tatattggag attgtactga
     8761 tgaggaatta aacgatcgtg aatttattgc atcagttgtt gacagggcta tttttcattt
     8821 cgcgattaat agtatatgta atcccgagga taataaagat gcgacactca tagaacgatg
     8881 tacttttgat gtagaaacca agaatggtct tccctccacg gttcagctat tttatgagga
     8941 atctaaagat aatgaacctt tagcgaatat acatttgcaa gcaataggtt ctggtttctt
     9001 aacgtttgtt aatgcctgcc aggaacatga tgacaacagc ttaaaattat ttgcttcgct
     9061 gttaatttcc ctttcatatt ctagtgtcta ctcagatttt gcaggaagag tgaatattaa
     9121 tgaatataat gataactacc tgaaagctca gtttgaagaa ttatctcaac gtgatatgaa
     9181 gaagtacctg ggagagatga agcgtctggc agatggggga gaaatgaatt ttgatggcta
     9241 tctggataag atgtcacatc tggtgaatga aggaacgctc gcccctgata ttttaagcaa
     9301 aatgcgagat gctgcaccga aattaattga ctttgctaag tcgtttgacc caaactcaaa
     9361 ggaaaagatt aaaatatata caggcacttc taaattaatt tatgatttat tcggggttaa
     9421 atcggagaaa taatatgtga agttcttcga tggtatgaaa ggtattacat aaaagaaccc
     9481 aatgtttatt gggttctttt tttctctatc agtgctatta gcagggagag atatcaccgg
     9541 agtttaatgt gtgattttta tttatcgtcg aatctgtatt gtttatcatt gcttttcaca
     9601 acgctaacat ctaataagat tagatattca cttcgtgaga gcttcatgcc ttgagaggat
     9661 ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt tatggtatag taccccgtta
     9721 ttgagcctcc tgaacagtga tgctgaataa cataaccaca tgatatatcg ataaaataat
     9781 ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa accagcgcaa
     9841 tgcctgaaaa aactgacgtt cactggagtg gtcggtttag cgttgcacca atgctcgata
     9901 ggatgtaccg tttttgaaaa acaaatagtt atatactttg tgggagccta ttgggaaccc
     9961 ggcgttttca tttcaaggtg taatccatac gcggcttaag aatgagatat aatgcgactt
    10021 ttagtgttcc gcttgagagg ccatgatgct taccctggac gagataggtc aatcagtacg
    10081 taacaatatc cagttgatta ttgatcatgt cggcttacct cttgctgttg gtccgctcag
    10141 tgatgatgat tacaagattc tgtgtggtgg ctatggtgag cttgaatggg actatgcgtt
    10201 aagtacctat ggcaactcca gagaaaagta tgagttctgc ataaaacttg ttcagcaagg
    10261 tcgggttcag ggaataccat caggagcagc aatttgtgtt tatggggttg aagaaaacat
    10321 ctttcgtatc catatgatcg aaaggttttc tagagaagat gaatctcacc cattgaaagg
    10381 gcgcatggtt ttactcactc ttatgagtgc ttttatattt tgtaaagctg ttgaatgtaa
    10441 agttgtccac attgtagagc cagtaccaga actggtgcag tattacgagt cttttggttt
    10501 ccgcatggaa cagtgcggtt atgtgatgtc ggcagtcatt gatgagctgc aggatatctt
    10561 tcttaaattt gctcagtagg tatagacgag aagggtctac aaattgtagg atacccgtcc
    10621 agattacctt aaaggtacat ctatggcagt cgttttgtgc ttaaactact aagaaacgat
    10681 gtcaccaatc gacatgatcg attggcataa gttagcgaaa caagctagct ttaaagaaag
    10741 ggttagagac gcctttactg tctcgggagt tttctatgaa agatcaaaaa gcaaccaagc
    10801 cacaggttaa gttcgacaca atgaaagcat tcgcaggtat gggtgctgct gttgaagttc
    10861 tgatgaaggc tgctcctaat gcgttcactc acgctactgt ctctggtaaa gagcagcagg
    10921 gtaagcttcg tcgtcgcaaa gcagcatgat catagctggt gctttttgaa aacccgcctt
    10981 caggcgggtt ttttctttag tgatgttctt tgtccttctg tttgactgtt ctgacctgtt
    11041 cccactcgat acgtccttct tctcgccttt tgtctatgta ttccgcaaga tcttgaatat
    11101 tgatgcaacg ttttgctttt tgtgatgtgc cgatgcgata tgttggaacg ggcaacttac
    11161 aagcgtttgc ttttgcttct gccgtggctg gactcatacc aaagtacttt tggctaactg
    11221 ctgagagttc aatgtttggg gtattgaatt cagccatcag taaaaacaag gtgttcataa
    11281 ttttctccat caaaaccggc tgcacccggg aaaatcataa ttctgtgctg gtggcaggaa
    11341 ttaatttctg ccagatagcg gaaacatatt ttgcctgatg acgggcatca gccagggcgt
    11401 tgtgccgttc gccatcgaaa ggcatgtcca ttttggggtc gaatccgatg gaacgcccaa
    11461 gcgtaacgat cgtgcgtaca tcgtggtcat tccagtacgc ccacgggcag atttgtcctg
    11521 ctcgctcgta agctccacgt aaaattacgt tgtcgaaggt ggccccgtta ccccagactt
    11581 ttaaatattt cgtattgtct gcgtgccggt taatgaaatg gtttagttct gagagagcat
    11641 cgctgatcga caaagtatca tcaatacttc cagcgatacc agcgcgactt taaatgcggt
    11701 aagtacgttt ttaaccacat cgattttgaa tactatttca tcacatacaa acgatttatc
    11761 gtctactacc gtttcaattc cggtaatcgt gttttgtagc catttggtta attcagccat
    11821 ttttcattac cgccctttcg ggcggtctcc tgatgttctg agggtgcagg aatccctccg
    11881 gttaaggatt aaattttatt tacagaactg aatttaatta ttcagatata cgtatctgta
    11941 accttacgaa cctactcact ggatgcctat ttcataaaaa taatccagtg ggttttatcg
    12001 ttttttcctg ttcgttgacc gataacaggt tttctgtcgg tcagtaccaa tatctggcga
    12061 acaggtattt gcgtttcatt ccatttaaaa atcagaacgc cggatggacg caacacacga
    12121 aaggcttctt taaatccctg ccgcaaatca tcacgctagg tatctttatt cagccgtcca
    12181 tatttctttc ccatccaggc gttatcacca acacgctcaa gatgcggagg gtcgaatata
    12241 accatcgaaa aagatgcgtc tgcaaatggt agtgcacgaa aatcagctat cagatcagga
    12301 ctgataatca ggcgtcgtcc atcacacaat gtgtgctctt cctttctgat atcgctaaat
    12361 atcgcccggt cgtcattctt atcgaaccag aacatgcgac tgccacagca catgtcgagg
    12421 attgctgcat gtccagtcac tggctgcctc ctttgcgaag ccgttccgcc cattcttcaa
    12481 gggatttctc tgcatattca ccggacaggc catcaatcgg atgcgattca ttagctaact
    12541 cttcttttgc tgacagaatc atgcgtgtaa cgtcgaaaac ttcagccaaa ggcttattga
    12601 taaatccgtg attgaaagca gcagcaagac ggcttgcggt atagttaata ccctcgttgc
    12661 gagcctcagc acgtacttca tcgaatttac gcaccagata ctcagcattt gtttcattca
    12721 ctttcagatc tcgcggtaca catttcccgc gaagaaaccc ttccatttcg aaaacattca
    12781 tgcgcatttg tgtaactccg ataactcgtt aaaacgttcc ataaacatcc cgtaggcatg
    12841 gctaggtgcc agtggaatca cgttgaacat ctctgttgcc gggatgcctt ccagtacagg
    12901 ccagaaagag ccatcatcaa gcccgagatc gcggcgttcg gttgccagca tgataagatc
    12961 ggcatatttc acgggcgtac tcataactgg gggtaacccg tatttctcac ggattacggc
    13021 gtcaattttt tcttccatcc gtttatagtc aggaagaagg cgtttcagtg gagcggggat
    13081 gtcctggcaa tacgcttctg ttgcatcatg cattaatgct tcaaaagcaa attcctgcgg
    13141 taccagctgg ctgcaaagaa ccgcatgttg ggcgacgctg tagaagtgcg aaagatgacc
    13201 ggcaaagcga cagatatttg aaagggaaac cgcgatatcg ttaatatcga tgtcgtcttt
    13261 atttatcctg tcataataaa aatgcttccc ggaaaaagtt ttaataaatg acattttgtt
    13321 ctccacgtat atgcgctgca ccgcgctgaa ttctggtaaa aagaatccct caccatccgg
    13381 cgattattga gtaaattacg tttccataaa tgccccctca ggggcatttg cagtaatgaa
    13441 atcaggcggt gaaagtacca ataaaggttt ctactttgct gtccttgaat ttctcaacaa
    13501 gcagatcacg aaattcgtta gccatttctt cctgcaccgc ctccagctga ataatgcgca
    13561 gaaccagtac cggacgatcg ccagtgataa tgctgaggcg taatttaaac ggacgttctt
    13621 tcagaccttc aaacggaatg catttaaatt caaatgccac tggcataatg tctttggtct
    13681 tcgcttcgac agactccatc agggagcgtt tgccgctgaa gtcattatct tcaaaatcag
    13741 cggtctggtt tgcttcaatc gtgattttac ggaccgccgc agccgctttt gttgcctgaa
    13801 tggtgtcacc attagcatca aagcccacaa ggtagtcggc ccagtcttca atccattctg
    13861 gttgccgcca atcgtatcgc gtttattgac ccggcaaacg ggaatgaaac gccgatgttt
    13921 gtggcgcagg gcaaccagat attcatgaac gacgtgttcc tgaagcgcct gacggccccc
    13981 accattacca gtggtggaaa tccaccggca ttttccctga caccggatgg aaagctgact
    14041 gctaaaaatg cggatatcag tggtaacgtg aatgcgaact ccgggacgct caacaacgtc
    14101 acgattaacg agaactgtcg ggttctggga aaactgtccg cgaaccagat tgaaggcgat
    14161 ctcgttaaaa cagtgggcaa agctttcccc cgggactccc gtgcaccgga acggtggcca
    14221 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgaccggca gattgttatt
    14281 ccggcggtgg cattcagtgg cgctaagcat gagagagagc atactgatat ttactcctca
    14341 tgccgtctga tagtgcggaa aaacggtgct gaaatttata accgtaccgc gctggataat
    14401 acgctgattt acagtggcgt tattgatatg cctgccggtc acggtcacat gacgctggag
    14461 ttttcggtgt cagcatggct ggtaaat
//
LOCUS       DAZGXS010000113        14387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGXS010000113 DAZGXS010000000
VERSION     DAZGXS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            115..633
                     /gene="yfiR"
                     /locus_tag="ACHKG5_003615"
     CDS             115..633
                     /gene="yfiR"
                     /locus_tag="ACHKG5_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0869633.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            623..1849
                     /gene="dgcN"
                     /locus_tag="ACHKG5_003616"
     CDS             623..1849
                     /gene="dgcN"
                     /locus_tag="ACHKG5_003616"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0869634.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1865..2347
                     /gene="yfiB"
                     /locus_tag="ACHKG5_003617"
     CDS             1865..2347
                     /gene="yfiB"
                     /locus_tag="ACHKG5_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0869635.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2424..2771)
                     /gene="rplS"
                     /locus_tag="ACHKG5_003618"
     CDS             complement(2424..2771)
                     /gene="rplS"
                     /locus_tag="ACHKG5_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0869636.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2813..3580)
                     /gene="trmD"
                     /locus_tag="ACHKG5_003619"
     CDS             complement(2813..3580)
                     /gene="trmD"
                     /locus_tag="ACHKG5_003619"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0869637.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3611..4159)
                     /gene="rimM"
                     /locus_tag="ACHKG5_003620"
     CDS             complement(3611..4159)
                     /gene="rimM"
                     /locus_tag="ACHKG5_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0869638.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4178..4426)
                     /gene="rpsP"
                     /locus_tag="ACHKG5_003621"
     CDS             complement(4178..4426)
                     /gene="rpsP"
                     /locus_tag="ACHKG5_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0869639.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4563..5924)
                     /gene="ffh"
                     /locus_tag="ACHKG5_003622"
     CDS             complement(4563..5924)
                     /gene="ffh"
                     /locus_tag="ACHKG5_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0869640.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6091..6882
                     /gene="ypjD"
                     /locus_tag="ACHKG5_003623"
     CDS             6091..6882
                     /gene="ypjD"
                     /locus_tag="ACHKG5_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0869641.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6904..8190
                     /gene="yfjD"
                     /locus_tag="ACHKG5_003624"
     CDS             6904..8190
                     /gene="yfjD"
                     /locus_tag="ACHKG5_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0869642.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8245..8838)
                     /gene="grpE"
                     /locus_tag="ACHKG5_003625"
     CDS             complement(8245..8838)
                     /gene="grpE"
                     /locus_tag="ACHKG5_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0869643.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8961..9839
                     /gene="nadK"
                     /locus_tag="ACHKG5_003626"
     CDS             8961..9839
                     /gene="nadK"
                     /locus_tag="ACHKG5_003626"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0869644.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9925..11586
                     /gene="recN"
                     /locus_tag="ACHKG5_003627"
     CDS             9925..11586
                     /gene="recN"
                     /locus_tag="ACHKG5_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0869645.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11735..12076
                     /gene="bamE"
                     /locus_tag="ACHKG5_003628"
     CDS             11735..12076
                     /gene="bamE"
                     /locus_tag="ACHKG5_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0869646.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12138..12428)
                     /gene="yfjF"
                     /locus_tag="ACHKG5_003629"
     CDS             complement(12138..12428)
                     /gene="yfjF"
                     /locus_tag="ACHKG5_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0869647.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12418..12855)
                     /gene="ratA"
                     /locus_tag="ACHKG5_003630"
     CDS             complement(12418..12855)
                     /gene="ratA"
                     /locus_tag="ACHKG5_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0869648.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13026..13508
                     /gene="smpB"
                     /locus_tag="ACHKG5_003631"
     CDS             13026..13508
                     /gene="smpB"
                     /locus_tag="ACHKG5_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0869649.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13723..14085
                     /gene="ssrA"
                     /locus_tag="ACHKG5_003632"
     tmRNA           13723..14085
                     /gene="ssrA"
                     /locus_tag="ACHKG5_003632"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3646 a         3788 c         3381 g         3572 t
ORIGIN      
        1 actcattatg ttttatttct cacaatgatg acgaccgcat gaaataatga tattttaatg
       61 agcattctga aaaaatatcg atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt
      121 ttttctcacc gactgttctt actgctgatt ctcctgctga cgggagcacc aatactcgct
      181 caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac
      241 acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacgtttt
      301 agtaccgccc tacaagagaa tgccgcaaca tctttgcctt atctgcctgt catcattcac
      361 actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa cgagtcaccg
      421 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa
      481 caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa taatgacgtc
      541 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
      601 ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac
      661 gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta tgatgctgat
      721 ctggttgctg ctttccgtga cctcggtgct gcccctgaaa cagtacgcgc aaaaaaacct
      781 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
      841 ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt caactgcaga
      901 agtacgtgat aagcagcaaa atattctggc atcctggcat tacacccgta aggatccagg
      961 cgatactttc agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat
     1021 tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag
     1081 ccattttatc tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggaat
     1141 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac
     1201 cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg
     1261 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
     1321 gtggcagctt cgtttacagg ccaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc
     1381 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa
     1441 ttccgctgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat
     1501 caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatcg caaaacggtt
     1561 agctgaattt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat
     1621 ggtgctctat ggtgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca
     1681 aatctttaat ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat
     1741 tggttacgca atgaccattg cgcacgcttc tgcggaaaaa ttacaagagc tggccgatca
     1801 caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat
     1861 cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg
     1921 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
     1981 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
     2041 tgactacaaa ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc
     2101 ctcgacaggg ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag
     2161 ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctatggg
     2221 tggacaaatt ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc
     2281 cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattactac
     2341 cccttaaaac acgagcaaac agataaaaaa agggccagcc aattggccag cccttcttaa
     2401 caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca
     2461 gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa
     2521 atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa
     2581 attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc
     2641 tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta
     2701 tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata
     2761 atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg gcttacgcca
     2821 tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg
     2881 cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc
     2941 ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg
     3001 gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc
     3061 cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc
     3121 gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc
     3181 cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc
     3241 gaccgcacac cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa
     3301 gcttgcgtcc ctgtggtgaa agataaatca cctttgcgcc ttcacccgcc gcggcttttg
     3361 ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt
     3421 aaggacgatc gtccacggta cggtgccggt catgcgtgaa atcgcgagga ctccagctct
     3481 ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc
     3541 ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt
     3601 atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga
     3661 ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat
     3721 ctttcaggtt tgccttaatg acgagaacgt cattggatcc ggtttccatc atatcgacga
     3781 ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt
     3841 agtagtcacc ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg
     3901 tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat
     3961 tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc
     4021 agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc
     4081 cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg
     4141 cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt
     4201 tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc
     4261 gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa
     4321 cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga
     4381 acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg
     4441 tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa
     4501 aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc
     4561 atttagcgac caggaaatcc cggcggcatc atccccttca tgcttctcat catcttcgcc
     4621 attccgccct tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg tttcagaaga
     4681 cggttaacgt cctgcacctg cataccgcaa ccggcagcaa tacggcgttt acgcgaacct
     4741 ttgatgattt ctggcttagc gcgctctttc atcgtcatcg agttgatgat ggcttccata
     4801 cgcaccagca ctttatcgtc catctgtgac ttgacgttat ccgggatctg ccccatgccc
     4861 ggcagtttgc ccatcaggct ggccatgccg cccatatttt tcatctggcg cagttgctca
     4921 aggaagtcgt tgagatcgaa gccgtcacct tttttcagct tgctggctaa tttctctgcc
     4981 tgcgcgcggt caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg
     5041 agaatacgcg acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca
     5101 acaccgagga acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg
     5161 cgggcatcgc cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat
     5221 gcttttgccg tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca
     5281 accgggttaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
     5341 agacgaccag cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
     5401 gccgcgttaa caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc
     5461 acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa
     5521 accaccagca ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg
     5581 gttgttttac cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc
     5641 agattcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg
     5701 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
     5761 acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg
     5821 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
     5881 ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg
     5941 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
     6001 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
     6061 tttcttattg ctcaaactgt cgacatcact atgcccgttt ttgctctgct cgcgcttgtc
     6121 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
     6181 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
     6241 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
     6301 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
     6361 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc aaccttcatg
     6421 cccaatgaat acatcaccca tctggaagct acgcctggga tgctggtgca cattggctta
     6481 tcgctctttt cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg
     6541 attgattacc aactgaagaa caagaagctg gcgtttaacc aggaaatgcc gccattgatg
     6601 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg
     6661 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggct
     6721 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tatggggaca ttatcatgaa
     6781 ggatggcgtg gacgtcgcgt cgtctggttt aacgttgcgg gtgcagtcat tctgacactg
     6841 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
     6901 cccctggaac acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt
     6961 tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtctgcga
     7021 catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca
     7081 gaccgcctga taagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg
     7141 ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt
     7201 gtgctgactt ttgtcgtact ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg
     7261 tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctcccctgca aattttgatg
     7321 atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc
     7381 aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcac
     7441 gaatcgcgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg
     7501 gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg tattgatatc
     7561 aacgatgact ggaaatctat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg
     7621 ctctaccgtg attcgctgga cgacgccatc agtatgctgc gagtacgtga agcttggcgg
     7681 ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc ggacgagatc
     7741 tattttgtgc cggaaggtac gccgctcagc acgcagttgg tgaagtttca gcgcaacaaa
     7801 aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa
     7861 gatattctgg aagagattgt cggcgatttc actacgtcga tgtcgccaac acttgccgaa
     7921 gaagtcacgc cgcaaaacga cggttcggtg attattgatg gcactgccaa cgtgcgggaa
     7981 atcaacaaag cctttaactg gcatctaccg gaagatgatg cccgcacggt taatggcgtc
     8041 attcttgagg cactggaaga gatccctgtc gtaggcaccc gcgtgcgtat tggcgagtac
     8101 gatatcgata ttctcgacgt acaggacaat atgattaagc aggtaaaagt ttttcctgtg
     8161 aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc ccggcattcg catgcagggc
     8221 cgtgaattat tacgaaaaca gaaattacgc ttttgctttc gctacagtta ccatcgccgc
     8281 acgaatcgta cgaccattca gcgtataacc cttctgcata atgcccagta cgttacctgg
     8341 cgcaacgtca tcagattcca ccattgcgat agcctgatgc acattcgggt ccagcggcac
     8401 gttagtttcg gcgatcactt caacaccaaa cttacgcaca acatccagca tcgacttcag
     8461 cgtcagctca atgccttcaa ccatcgcaga catatccggg ttagctttat cagccacttc
     8521 cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt tctccagcgc
     8581 gaatttgtgg gctttttcaa tatccagttc agtacgacga cgcaggtttt ccatttcggc
     8641 ttttacacgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct gagcttcgag
     8701 attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcttctg gctcaactgc
     8761 ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct caggcgtttt
     8821 ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc gctaacttcg
     8881 cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc atcaatcttc
     8941 gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt gtgggacacc
     9001 cacggcaccc tactgcactg acaacacatg aaatgctcta ccgctggctg tgcacaaaag
     9061 gttacgaggt catcgttgag caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa
     9121 ctggcacgct cgcggagatt gggcaactgg ctgatctcgc ggtagtcgtt ggtggcgacg
     9181 gtaatatgct gggcgcgacg cgcacgctcg cccgttacga tattaaagtt attggaatca
     9241 accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag caacaattag
     9301 ccgatgtgct ggaaggtcac tacatcagcg agaaacgttt tttgctggaa gcgcaagtct
     9361 gtcagcaaga ttgccaaaag cgcatcagca ccgcgataaa tgaagtggta cttcatccag
     9421 gcaaagtggc gcatatgata gagttcgaag tgtatatcga cgagatcttt gcgttttctc
     9481 agcgatctga tgggctaatt atttcgacgc caacaggctc caccgcctat tccctctctg
     9541 cgggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc atgttcccgc
     9601 atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt ctgcgttttt
     9661 cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcacta ccgattcagg
     9721 aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt catccgaaag
     9781 attacagtta tttcaacaca ttaagcacca agctcggctg gtcaaaaaaa ttattctaat
     9841 tttacgccag cctctttact gtatataaaa ccagtttata ctgtacacaa taacagtaat
     9901 ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc
     9961 gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg cgaaaccggt
    10021 gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc
    10081 gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat
    10141 acgccagcgg ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg
    10201 cttcgtcgcg tgatcagcag cgatggtcgc tcccgtggtt tcatcaacgg tacagctgtt
    10261 cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct
    10321 catcagttac tcaccaaacc cgagcaccaa aaattcctgc ttgatggcta tgccaatgaa
    10381 acctctctac tccaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac
    10441 ctcgcgcatc atcaacagtt aagtcaggaa cgcgctgccc gtgcggaact gctgcaatac
    10501 caattaaaag aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa
    10561 gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca
    10621 ttaatggccg acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg
    10681 gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa
    10741 gctaccatcc agattgttga agccagcgac gaactgcgcc actactgcga tcgtctggat
    10801 ctcgatccca accgactgtt tgaacttgaa cagcgcatct caaaacagat ttcgctggca
    10861 cgtaaacatc acgtcagccc tgagacattg ccacagtatt accagtcgct actggaagaa
    10921 cagcagcaac tggacgatca ggccgactca caagaaacgc tcgcactggc ggtaacgaaa
    10981 catcatcagc aggcactgga aacggcgcgc gcattacacc agcaacgcca gcattatgca
    11041 gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag
    11101 tttacgattg atgtcaaatt tgacgagcat cacctgggcg ctgacggcgc cgatcgtatt
    11161 gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc
    11221 ggtggtgaat tgtcccgcat cgcattggca attcaggtca tcacggcgcg taaaatggaa
    11281 accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt
    11341 gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg
    11401 ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg
    11461 atgacagaaa cgcatatgca atccctgaat aaaaaaacgc ggttacaaga gctggcgcgc
    11521 ctgcttggtg gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgcg
    11581 gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa
    11641 agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac
    11701 gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc
    11761 agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag tggtttaccg
    11821 tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg
    11881 catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg
    11941 tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca
    12001 aacgctgacg ctaaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc
    12061 gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag
    12121 cacctttttt ctgtccgtta tttattcgct gatttttctg ctcgttgcct gcgaagctct
    12181 ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg
    12241 cttaattttg ccggacggct gtaaatgccg actttgtttt cagttaaatc gatatcggta
    12301 cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc
    12361 gtcacgcgct gcaggtactg cttcttaggt agcgcataag ccacctcaac ggcaatttta
    12421 cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagacgccag
    12481 ctctttaaac acgcgaccaa aagcgagttc aatcaactta ttggtaaact caaagtcgag
    12541 atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc caccaatcaa
    12601 tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt
    12661 gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg
    12721 cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact gaggataaga
    12781 ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg
    12841 gctaatctga ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc
    12901 tgcagtaaaa aaaacgctat cccggcgctg ggtaacatcc ggttcatgct aagatagagc
    12961 cttgtccccc gcaggattga tatggggtat tttcgatttc agattaccga tgattcacga
    13021 cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaacaagc
    13081 gcgcccgtca cgaatacttt attgaagaag agttcgaagc gggacttgcc ctgcaaggct
    13141 gggaagttaa atccctgcgc gcaggaaaag ccaatattag cgacagctat gtccttctgc
    13201 gcgacggaga agcgttcctg tttggcgcaa acatcacgcc aatggctgtg gcttctacgc
    13261 atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc gaacttgatt
    13321 cgttgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga
    13381 aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taagaaacag cacgataaac
    13441 gttcagatat caaagaacgc gaatggcagg tggataaagc gcgtatcatg aaaaacgccc
    13501 accgttaaac ctgcactcca attcttgacc tgttcctcac cgcgcctccc tctccggcgg
    13561 cgcgaatgaa catcttattg gctatcacat ccgacaaaaa tgttgccatc ccattgctta
    13621 atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg
    13681 gtcatagcgc tcataaatct ggtatacttg ccgttacaca ttggggctga ttctggattc
    13741 gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc
    13801 aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc
    13861 ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat
    13921 cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg
    13981 gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg
    14041 acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccacttta agaaggacta
    14101 caaccggaca gtagcaataa atacagccac ttacggacac aagccagaca gtagtcggac
    14161 tgaaaaaaga caaaaatata cacgtgaaat gcacgtgcac ttctaaaaaa ccccagttat
    14221 caccgactgg ggtttttcaa tttgtaacta agggtaacaa ccccccgttt ttttcatctc
    14281 actcaccttg atactgttta tttgtacagt taaaaataat actgtataca aacacagtat
    14341 agagggactt ttatgcgtat tgagatctgc atagccaaag aaaaaat
//
LOCUS       DAZGXS010000114        14236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGXS010000114 DAZGXS010000000
VERSION     DAZGXS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            106..1219
                     /gene="ompN"
                     /locus_tag="ACHKG5_003633"
                     /pseudo
     CDS             106..1219
                     /gene="ompN"
                     /locus_tag="ACHKG5_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1360..1794
                     /gene="uspF"
                     /locus_tag="ACHKG5_003634"
     CDS             1360..1794
                     /gene="uspF"
                     /locus_tag="ACHKG5_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0869650.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1970..2905
                     /gene="ttcA"
                     /locus_tag="ACHKG5_003635"
     CDS             1970..2905
                     /gene="ttcA"
                     /locus_tag="ACHKG5_003635"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0869651.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3034..4407)
                     /gene="dbpA"
                     /locus_tag="ACHKG5_003636"
     CDS             complement(3034..4407)
                     /gene="dbpA"
                     /locus_tag="ACHKG5_003636"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP0869652.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4440..4610)
                     /gene="ynaL"
                     /locus_tag="ACHKG5_003637"
     CDS             complement(4440..4610)
                     /gene="ynaL"
                     /locus_tag="ACHKG5_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0869653.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4885..5868)
                     /gene="zntB"
                     /locus_tag="ACHKG5_003638"
     CDS             complement(4885..5868)
                     /gene="zntB"
                     /locus_tag="ACHKG5_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0869654.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5882..5929)
                     /gene="ydaN"
                     /locus_tag="ACHKG5_003639"
     CDS             complement(5882..5929)
                     /gene="ydaN"
                     /locus_tag="ACHKG5_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0869655.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6123..7355
                     /gene="dgcM"
                     /locus_tag="ACHKG5_003640"
     CDS             6123..7355
                     /gene="dgcM"
                     /locus_tag="ACHKG5_003640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0869656.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7376..7939)
                     /gene="smrA"
                     /locus_tag="ACHKG5_003641"
     CDS             complement(7376..7939)
                     /gene="smrA"
                     /locus_tag="ACHKG5_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0869657.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8269..9177)
                     /gene="abgR"
                     /locus_tag="ACHKG5_003642"
     CDS             complement(8269..9177)
                     /gene="abgR"
                     /locus_tag="ACHKG5_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0869658.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9353..10663
                     /gene="abgA"
                     /locus_tag="ACHKG5_003643"
     CDS             9353..10663
                     /gene="abgA"
                     /locus_tag="ACHKG5_003643"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP0869659.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10663..12108
                     /gene="abgB"
                     /locus_tag="ACHKG5_003644"
     CDS             10663..12108
                     /gene="abgB"
                     /locus_tag="ACHKG5_003644"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP0869660.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSPLK"
     gene            12145..13671
                     /gene="abgT"
                     /locus_tag="ACHKG5_003645"
     CDS             12145..13671
                     /gene="abgT"
                     /locus_tag="ACHKG5_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP0869661.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13682..14197
                     /gene="ogt"
                     /locus_tag="ACHKG5_003646"
     CDS             13682..14197
                     /gene="ogt"
                     /locus_tag="ACHKG5_003646"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0869662.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3584 a         3570 c         3493 g         3589 t
ORIGIN      
        1 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
       61 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagcaaagta
      121 ctggcacttt taattcctgc cctgctcggc gcaggtgctg cacatgcagc cgaagtttat
      181 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
      241 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
      301 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
      361 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaattttca
      421 gattacggtt ctttcgatta cggacgtaat tatgggctgg accgatatgc tgcctgaatt
      481 tggcggtgac tcttatacca atgcagacaa ctttatgact ggtcgtgcca atggcgtcgc
      541 gacttatcgt aatactgatt tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta
      601 tcaaggtaac aacgaaggtg ccagtaatgg tcaggaaggc accaacaacg gacgtgatgt
      661 tcgccatgaa aacggtgacg gctggggtct ttccacaaca tatgatttag gcatgggctt
      721 tagcgctggt gcggcataca cctcttctga ccgcaccaat gaccaggtta accatactgc
      781 ggcgggtggt gataaagcag acgcgtggac tgctgggcta aaatacgatg ctaacaatat
      841 ttacctggca accatgtatt cagaaacgcg taatatgacc ccgtttggcg acagcgatta
      901 tgctgttgca aacaaaaccc agaactttga agtcactgca cagtaccagt ttgattttgg
      961 tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt gacctgcacg ctgccggtgg
     1021 tgcagacaac ccggcaggtg ttgatgataa agatctggtt aaatacgccg atgttggcgc
     1081 gacttactat ttcaataaaa acatgtccac ttatgttgac tataaaatca acctgttgga
     1141 tgaagatgac agtttctacg ctgccaacgg catctctacc gatgatattg tcgctttagg
     1201 tctggtttat cagttctaaa tcctcctgcc cgctgttatg gcgggctttt tctgcttatt
     1261 cttcctcctt tgatctaaat taaaaatgtg aactccgtca ttacacaaaa agtgtcatct
     1321 ggcgttacac tttatgcgga tactaaaaca ggaggtttta tgaacagaac gattcttgtc
     1381 cctatcgata tttccgattc agaattaact caacgcgtga ttagccacgt tgaggcagag
     1441 gcaaagattg atgatgcaga ggttcatttc ctgacggtaa taccttcact gccctactat
     1501 gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa tggatgacct gaaagcggaa
     1561 gccaaatcgc aactggaaga gatcattaaa aaatttaaac tgccaaccga cagagtgcat
     1621 gtccatgttg aggaaggctc gcccaaagac cgcattctgg aattggcgaa gaagatcccc
     1681 gctcatatga tcatcattgc ttcccatcga ccggatatca ccacttatct gctcggttcc
     1741 aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg ctgacactaa
     1801 cgcccgcaca ttgctgcggg ctttttgatt catttcgcaa atgtgctgac attttcccct
     1861 ctaatccgta ccatacacgc cacagttttt atatcagatt tcttatgctg ggcgttccgg
     1921 catgacgcat actcttctga tgccatataa cgaattgagt cgcttttaaa tgtcgcaaaa
     1981 tcaagaaatt agcaagaaag aacaatacaa cctgaacaaa ttacaaaaac gcctgcgtcg
     2041 taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc gcatcatggt
     2101 ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca atttgcagca
     2161 aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc aaccgggctt
     2221 cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca agattgttga
     2281 agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca cttgctcact
     2341 gtgttctcgg ctacgtcgcg gtatccttta tcgcaccgca acggaactgg gggcgacgaa
     2401 gatcgcgttg ggccaccatc gtgacgatat cctgcaaacg ttgttcttaa atatgttcta
     2461 cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca aacatatcgt
     2521 tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg atgcaaaagc
     2581 gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac gtcaggtgat
     2641 tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga cgatgttcag
     2701 cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg atttcaaagg
     2761 catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc gcgaagagat
     2821 cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg atgagttacg
     2881 gctgaatgtg gttgaagtga aataactcgg atagcgcccg atgcgcaaac gtatcgggct
     2941 actctaatgg aggctgaata agacgcggtc agcgtcgcat ccgacaatcc cgacgaagat
     3001 gtttactttt gcatcaggca attcaacatt ttattatttt aataaccgca cccggcacgt
     3061 ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa cagcctgacg
     3121 gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg cgccatcaag
     3181 cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg cttttttccc
     3241 gccatcgata cacaacgttg ccatttctgc ttccagcgtc acaatggaac tattagctgg
     3301 tggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg cccgctgtgc
     3361 ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag ctgtacgacc
     3421 gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca ccagctcaag
     3481 cgatttaata tccagaccac gcgcagcaac atcagtcgcg accagtacac gggcgctacc
     3541 gttagcaaaa cgtaccaggg tctgatcacg atcgcgttgc tccagatcgc cgtgtaatga
     3601 caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat cttttttggt
     3661 attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt gcaacagagg
     3721 aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca aagcatctgt
     3781 tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg cggcgatggc
     3841 ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa aacggatgac
     3901 atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat ccatcaccaa
     3961 agtattcagc gcatccagtg ataccgtgcc tttttgcagg tgatccagca aacggcccgg
     4021 cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac cgaacggttg
     4081 accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc
     4141 acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct gggtttgaaa
     4201 tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc
     4261 agttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg
     4321 caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg cgggaggcaa
     4381 aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagtcat
     4441 caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg ttcttcatcg
     4501 ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg aacaggatcg
     4561 ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc
     4621 ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga
     4681 ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt
     4741 aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca agagagatat
     4801 tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt ttttgtatac
     4861 agacagccgg aaaaattgct tttgttacaa ccatttacta cgatgcaacc ataaagcaac
     4921 accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt gccacccgcc
     4981 gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaaggtac tgggtaaaaa
     5041 gaccattgcc atcaatgaca ttgtataggt acgacgagct aaattttcct gcatcacctg
     5101 agcgatttca tccgccatca caccagtccg tgctatacag gcgtcgattt cgtcaaggcc
     5161 gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg
     5221 caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat
     5281 cacaattaat tgtttgcgca gcagagccag gaatccacgc ggcggaattt gctgatcaag
     5341 gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt cgctggaatg
     5401 atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg gacccgtgcc
     5461 ctcttccaga tcactcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac
     5521 aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt
     5581 gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc tgactcgggg
     5641 ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc
     5701 cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc
     5761 atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccaggc
     5821 aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag tgcctccatc
     5881 atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc
     5941 attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt
     6001 tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc
     6061 atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat aatgggatct
     6121 caatgattac gcacaacttc aatactctgg acttactcac cagtcctgtc tggatcgttt
     6181 cgccctttga ggaacagtta atttatgcca acagcgcggc gcgactgttg atgcaagacc
     6241 tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa gaactgccga
     6301 aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact gttcagcgta
     6361 aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca gaaacagaac
     6421 cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa gccagtcgct
     6481 cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg actaactctg
     6541 caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct aacctcgccg
     6601 cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc tgggaaataa
     6661 atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ctcgcattta cccggtggtc
     6721 ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat gtgcagacct
     6781 atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta
     6841 ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac gcgatgaccg
     6901 gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg cagcacctcg
     6961 ccatcgctca ggattacagc ttattgctca tcgacacaga tcgttttaaa cacattaacg
     7021 atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc actctcgaaa
     7081 gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtcttattac
     7141 taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc gtaagcgtgg
     7201 caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg gcgcatcacg
     7261 aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgggcga
     7321 aaaatgatgg acgcaaccgc gtgctggcgg cataagccac ggatgcgtct cgagatcaac
     7381 gactgcgctt agcgtggcgc tcccagtttt cttgctttgc ctgtgccgtt ttacgtagtg
     7441 cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtacaatat gcctggacat
     7501 catcaaattc ggtcaaccag cgcgccacat agctgcggac aatattggca tgcgatttat
     7561 catcccgccc tttaccatga ataatcagca cgttacgcaa accatccgcc atggcttgtt
     7621 gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc
     7681 tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt tgcaaccctt
     7741 cccgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg gtggtgagga
     7801 aattatcaag ttgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc
     7861 agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg
     7921 atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata tgataaccag
     7981 accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca aatattgccg
     8041 atattttaag caaaatgctt atgcatgatt attcattcac gatattaata atgtaactta
     8101 tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa ccgttataac
     8161 acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac tgctgtgtgt
     8221 actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt aactttgcag
     8281 atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg tctggcgact
     8341 atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa ccggcaacat
     8401 caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta tgctgagaaa
     8461 atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta caccgacctg
     8521 tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtagc tgccgtgtgg
     8581 cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac caatggcggg
     8641 gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa aagtaaattc
     8701 gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc cctgacgcaa
     8761 ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct gcggatgctg
     8821 ctgatgaaaa cgagatatta cagctggcat cagactgcgg gaaatactgg cccccatgcc
     8881 gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt gggctgcgcg
     8941 cagttcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat cagttaacgt
     9001 cacgccttta ctacggcgaa aaaagagttg cgccgctaac ccttcttcca gctcctgaat
     9061 agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc gaatgctgcc
     9121 ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa aagccatgac
     9181 gccaccgata accgttattt atcagaccaa agaaactggc atctacttta atgcagatga
     9241 ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt gataagtaaa
     9301 aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg ttatggagtc
     9361 tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac gtgattttca
     9421 tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg cggaagaatt
     9481 gcagcagctc ggctactcac tggcgctggg ccgcgaagta gttaatgaaa atagccggat
     9541 gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc aacagggtgc
     9601 gctagcacaa tggattgcgg cttttgaagg tggtttcacg ggtatcgtcg ccaccctgga
     9661 taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc tggatctcag
     9721 tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat gtaacgccgg
     9781 aatgatgcat gcctgtggtc atgatgggca taccaccatt gggcttgggc tggcgcatac
     9841 ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc agcctgcaga
     9901 ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg atgttgatta
     9961 ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt gcggcagtga
    10021 taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg ctcacgcagg
    10081 cgcaaaacca gaagacggtt acaatgcctt gttggcggca gcacaagcca ctcttgcact
    10141 gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg ttatgcaggc
    10201 aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa cacgcggggc
    10261 cagcgacgtc attaatcaat atgtttttga ccgtgctcag caagcgattc agggcgcagc
    10321 aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg ccagttctcc
    10381 ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg gggtcaatca
    10441 ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa tgatggcccg
    10501 cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc tggcggcagg
    10561 tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg tcgaaacgct
    10621 ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga aatctatcgt
    10681 tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc cgatcaaatc
    10741 tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct ggcttcggcg
    10801 ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa tgcctttatt
    10861 gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga cgccctggca
    10921 ggtttaagtc agcaagcagg ttgcgcgcaa cctacatccg tgacgcccgg tgaaaatggt
    10981 cacggttgcg gacacaattt gctgggaacc gccgcctttg ccgctgcaat agccatcaag
    11041 aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg tcctggcgaa
    11101 gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga tgtggatgcg
    11161 gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgtac gctggcaaac
    11221 attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc ccctcatttg
    11281 ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt cctcaacgaa
    11341 catattattg aaaaagcgcg cgtacactat gccatcacaa atagtggcgg gatctcgccc
    11401 aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga aatgaccgac
    11461 gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt gatgaccgaa
    11521 accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa tcgcacctta
    11581 gaaaatgcca tgtaccaggc cctatcccat tttggtaccc cggaatggaa ctccgaagaa
    11641 ctggcttttg cgaaacaaat tcaggctacg cttacctcca acgatcggca aaacagtctg
    11701 aataatatcg ccgcaaccgg tggcgaaaac ggcaaggttt ttgcactacg tcatcgtgaa
    11761 acggtactgg cgaatgaagt cgctccatat gccgccaccg ataacgtgct tgcggcatcg
    11821 actgatgtcg gcgacgtcag ttggaaactg cctgttgccc agtgtttcag cccctgtttt
    11881 gccgtcggta cacccctaca tacgtggcaa ctggttagcc aggggcgaac atctattgct
    11941 cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacactcaa tctcttcatt
    12001 gattcagggc tattgcaaga atgccaacaa gagcatcagc aagttacgga cacgcaaccg
    12061 tatcactgcc ctatcccgaa aaacgtgaca ctgtcacctt taaaataaca acaacaacgc
    12121 aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc ctcccaatcc
    12181 gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc ttttttgctc
    12241 tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc ctttggcgtc
    12301 agtgcgaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct cagtgtggaa
    12361 ggattacact ggtttttacc caatgttatt aaaaacttta gcggttttgc tccacttggt
    12421 gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt actgccagca
    12481 ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat ggtgctgttt
    12541 attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc accgatgggt
    12601 gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc tgccattgca
    12661 ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt gttgctgtcg
    12721 gggatcagca cggaagcggc agctgcgttc aatccgcaaa tgcacgtcag tgtaattgat
    12781 aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg cctgataacc
    12841 gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga gaaactgcag
    12901 acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc gctacttttt
    12961 attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga tccgattaat
    13021 cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat tctttttttc
    13081 tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca ggcggattta
    13141 ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat ggtttttccc
    13201 ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat cgccgtgggg
    13261 ctgaccgata tcctggaaag ttcagggctt agcggcatcc cggcgtttgt cggtctggcg
    13321 ttgctttcct ctttcttatg catgtttatc gccagcggtt ccgcaatctg gtcgattctg
    13381 gcccccattt tcgtaccaat gtttatgcta cttggctttc acccggcatt tgcgcaaatc
    13441 ctctttcgta ttgccgactc atccgtattg cctttagcgc cagtatctcc ttttgttcca
    13501 ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac ttactattcg
    13561 ttagtcttgc cctatccgct tatctttttg gtggtatggc tgctgatgtt gctggcgtgg
    13621 tatcttgtgg gcctgccgat aggtccgggt atttacccac gtttgtctta agagagaacg
    13681 gatgctgaga ttacttgaag aaaaaattgc cacaccactg ggtccactgt gggtgatttg
    13741 cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac gcatggtgca
    13801 gctgctggac atccattatc gcaaagaagg ctatgagcgc atttctgcca ccaatccagg
    13861 cggtttaagc gacaagcttc gtgaatattt tgccggtaat cttagcatta ttgatacgct
    13921 tcccactgct acggggggga cgccatttca gcgcgaagtc tggaaaacac tacgcactat
    13981 cccctgcggg caggtaatgc attacggcga actggctgag caattgggcc gtcctggcgc
    14041 ggcgcgtgcc gttggtgcgg caaacggatc gaatcccatc agcatcgtcg taccttgcca
    14101 tcgggttatt ggccgaaacg gcaccatgac cggatatgca ggcggagttc agcgaaaaga
    14161 gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt tgtgccagct
    14221 tgttcacact tttatg
//
LOCUS       DAZGXS010000115        14168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGXS010000115 DAZGXS010000000
VERSION     DAZGXS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(246..761)
                     /gene="pgpA"
                     /locus_tag="ACHKG5_003647"
     CDS             complement(246..761)
                     /gene="pgpA"
                     /locus_tag="ACHKG5_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0869663.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(739..1716)
                     /gene="thiL"
                     /locus_tag="ACHKG5_003648"
     CDS             complement(739..1716)
                     /gene="thiL"
                     /locus_tag="ACHKG5_003648"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0869664.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1794..2213)
                     /gene="nusB"
                     /locus_tag="ACHKG5_003649"
     CDS             complement(1794..2213)
                     /gene="nusB"
                     /locus_tag="ACHKG5_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0869665.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2233..2703)
                     /gene="ribE"
                     /locus_tag="ACHKG5_003650"
     CDS             complement(2233..2703)
                     /gene="ribE"
                     /locus_tag="ACHKG5_003650"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0869666.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2792..3895)
                     /gene="ribD"
                     /locus_tag="ACHKG5_003651"
     CDS             complement(2792..3895)
                     /gene="ribD"
                     /locus_tag="ACHKG5_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0869667.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3899..4348)
                     /gene="nrdR"
                     /locus_tag="ACHKG5_003652"
     CDS             complement(3899..4348)
                     /gene="nrdR"
                     /locus_tag="ACHKG5_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0869668.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4499..5038
                     /locus_tag="ACHKG5_003653"
     CDS             4499..5038
                     /locus_tag="ACHKG5_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0869669.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5337..6221
                     /gene="tsx"
                     /locus_tag="ACHKG5_003654"
     CDS             5337..6221
                     /gene="tsx"
                     /locus_tag="ACHKG5_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0869670.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6398..6745)
                     /gene="yajD"
                     /locus_tag="ACHKG5_003655"
     CDS             complement(6398..6745)
                     /gene="yajD"
                     /locus_tag="ACHKG5_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0869671.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6833..7114)
                     /locus_tag="ACHKG5_003656"
     CDS             complement(6833..7114)
                     /locus_tag="ACHKG5_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0869672.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7107..7349)
                     /locus_tag="ACHKG5_003657"
     CDS             complement(7107..7349)
                     /locus_tag="ACHKG5_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0869673.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7501..8472)
                     /gene="secF"
                     /locus_tag="ACHKG5_003658"
     CDS             complement(7501..8472)
                     /gene="secF"
                     /locus_tag="ACHKG5_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0869674.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8483..10330)
                     /gene="secD"
                     /locus_tag="ACHKG5_003659"
     CDS             complement(8483..10330)
                     /gene="secD"
                     /locus_tag="ACHKG5_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0869675.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10358..10690)
                     /gene="yajC"
                     /locus_tag="ACHKG5_003660"
     CDS             complement(10358..10690)
                     /gene="yajC"
                     /locus_tag="ACHKG5_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0869676.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10713..11840)
                     /gene="tgt"
                     /locus_tag="ACHKG5_003661"
     CDS             complement(10713..11840)
                     /gene="tgt"
                     /locus_tag="ACHKG5_003661"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0869677.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11895..12965)
                     /gene="queA"
                     /locus_tag="ACHKG5_003662"
     CDS             complement(11895..12965)
                     /gene="queA"
                     /locus_tag="ACHKG5_003662"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0869678.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13058..13639
                     /gene="acpH"
                     /locus_tag="ACHKG5_003663"
     CDS             13058..13639
                     /gene="acpH"
                     /locus_tag="ACHKG5_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0869679.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13660..>14168)
                     /locus_tag="ACHKG5_003664"
     CDS             complement(13660..>14168)
                     /locus_tag="ACHKG5_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869680.1"
                     /translation="QTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCL
                     FDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVKS
                     EPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAA
                     PLDANAKK"
BASE COUNT         3529 a         3801 c         3404 g         3434 t
ORIGIN      
        1 ataattttga tgatattttt cgtcaacagt cagagaatat gagcaattat tatgagttcg
       61 tcgactgacg atatttcagt gtcattgaat tatgagtgac aaagatacac cttgaaatat
      121 cgccgcatag tatattcggc gatgtcgggt tttactttaa cagagtgccc caaaattggg
      181 gcactgttca tttataaatc aaaccgggtg gtctggaatc gcctacacct cccggtcaat
      241 ggcatttaaa gaatacccag cggccagtga tgaccgataa agtacaggat gcctgcggaa
      301 atcaccccgg cgacaatatc atcgatcatg atccccatgc cgccatgtac attgcgatca
      361 aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac aaacccggcg
      421 gcgacccact gccagtcatt ggtcggcagc gccatcaggg tgatccacat gccaataaac
      481 tcatcccata cgatgctacc gtgatcgtgc acccccatgt ctttcgccgt ttgatgacaa
      541 agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg ccagggcaaa
      601 aaggtcatca gataccagaa cggaatcgcc gccagcgatc ccatcgtgcc aggaacgatt
      661 gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact catcttcagg
      721 cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc agtctaacgt
      781 gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat
      841 acaggtaaac ggtacgccca gatgaccgag agccacatcc agcgcgccac ggttcagttc
      901 cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg
      961 ttccggtaca acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg
     1021 tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag
     1081 atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg
     1141 acggagatga cgtttgatca agtagtccgc atctttagca tcggcaacct gcaaacggtt
     1201 ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat agatccagtc
     1261 acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc
     1321 caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata
     1381 attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg
     1441 taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag
     1501 gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga ggaaatggtt
     1561 gcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag
     1621 agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa
     1681 ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa
     1741 agaaaaggcc ggcgatcgcg gaacagtctt ccgtgaatct accggcctgg atattacttt
     1801 ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg
     1861 tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc
     1921 acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt ttctacctgt
     1981 cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag gtatgcggta
     2041 ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc
     2101 tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag
     2161 tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc
     2221 cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag
     2281 tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat
     2341 gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc
     2401 caggccgttg cttgcaccac cagcgacata ttcaaaatgg gcagtgccac cacgaataac
     2461 cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag
     2521 cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt ttacctgacc
     2581 gatacgtttc agtgcatcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa
     2641 acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt
     2701 cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt
     2761 tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat
     2821 ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcagct aatttctcaa
     2881 gccctggcag tgtgcataat ccacgggcgt cgctgcctaa tagtttaggt gcgatataga
     2941 caatcagctc atcgactaac cccgcctgca gcaatgcgcc agcgagcgtt ggccccgctt
     3001 ccacccagat gctgttaatc tgctgtttac ccagttgcat catcagtaca accagatcca
     3061 gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat
     3121 cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgtga tgttccggcg
     3181 tcacgcgatt ttggctatca atcacaatac gtaccggctg gcggagatga tgttgcgggt
     3241 agagcgcctg agtttgtaca tccagttcag accaacgcac cgttaaggca ggatcatccg
     3301 ccagcaccgt agcgctgctg gttaaaatgg catgactttg tgcgcgcagt cgttgtacgt
     3361 cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc gccgtgcggc
     3421 catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga
     3481 gaaagccttt attcaattgc tcggcttcac tcatcatcag gccgtggctg acgtcaatgc
     3541 cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttcggg tcctgcatcg
     3601 cagcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt ggcgtacgac
     3661 catgatggct acagggttcg agtgtgacat aggcggtcgc acctttggct ttttcacccg
     3721 ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg taaccttcac
     3781 cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg tgggtggtaa
     3841 aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt
     3901 agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga
     3961 cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat
     4021 tgccaatcat cttgctcggc acttcgcgct caccggtggc gcgcagctgc gatttaatat
     4081 gattgattgc catttcgacg tcatcggaac tcaccggacg tttttccagc gcccgcagca
     4141 ttccgctacg taatttttct tcattaaacg gttcacgcac gtcgttgctt tttacaacac
     4201 gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact
     4261 gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt accttagtgt
     4321 ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacggaaatc
     4381 tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat
     4441 gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat
     4501 gaatattaac gtttttcgac tgatcctgct gggaagccta ttcagcctta gcgcctgtgt
     4561 gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac
     4621 gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg caaaatccag
     4681 cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa gccagatcgg
     4741 tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca ccctgacact
     4801 acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg ttgaatatgg
     4861 gcaaattcag ggaacaatag acaactttca ggaaatcaat gtacagaatc aattgattaa
     4921 tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacagt taaaggggat
     4981 ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac
     5041 ttatagcggg gcgattttgt gcgtcccgca acatccttcc ccatcatttt gttactccgc
     5101 ttacataacc cagattgata gtaaaagttt gcaacaaggg cgaaagtcag tacaatcccc
     5161 gcccgaatgt gtgcaaacgt gaacgcaatc gattacgtaa atgatagaac tgtgaaacga
     5221 aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga aatatttaga
     5281 aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc atacatatga
     5341 aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt actgtcaacg
     5401 cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc gttaacgttg
     5461 tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac cttgagtacg
     5521 aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg ccggtattct
     5581 tcggcggtaa ctccgatgca aaaggtatct ggaaccacgg ttctcctctg tttatggaaa
     5641 tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc ggtccgttca
     5701 aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa gatggtcgcc
     5761 agagcacctg gtacatgggt ctgggtaccg atatcgacac tggcctgccg atgagcctgt
     5821 ccatgaacgt ctatgcgaaa taccagtggc agaactatgg cgcagcgaac gaaaacgagt
     5881 gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg tggggcggtc
     5941 agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg gatgacagcg
     6001 gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc agccatattc
     6061 tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg cacgacggtg
     6121 gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac gttcgctcta
     6181 ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa tgccgggatt
     6241 tattcccggc atttcagatt gttaacaacg tacgctttgt tcatgccgga tgcggcgtaa
     6301 acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat aacgtaggcc
     6361 tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg ttcatcatcg
     6421 ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc gcatcttccc
     6481 ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc tcatgatcgt
     6541 ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg gtatggtcgt
     6601 gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg cgggaacagc
     6661 gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt tctaaccgcg
     6721 catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag ggttacgcgc
     6781 agtttcccaa gatgcgggga ttttcgcaat cctgagtcaa gaaattctca cattaccagt
     6841 gacggcgagg ttccatcaac tgatgaagaa tgcgcgcaat gagaatgtca tcgactctta
     6901 taacgtaata aacagtgtga aacctaaatg cgatcttctt tacctctgga atcgcatcat
     6961 agtcttgccc tatctcgggc atttgagaga gtgtatccag aaaagtgccg agagagtcgg
     7021 tatagcgatc cgcctgctca tcaccaaact ttcccagcgt gtagtcatag atccctctga
     7081 aatcttcaac agcttttccg ctaagcttat acacgatgtt tttgcttcct ttctgctgcg
     7141 atatcacgaa gcgtcatcga actttcaccg ctctcaagac cttcaataac agcatttctg
     7201 acagcttcat ccctggattc ttgttgttcc aatagacgaa gtgccgaacg cattacttca
     7261 ctggccgagc cataacgacc agactggacc atccgggtaa taaaactatc taattgttcg
     7321 ccaacgctta ctgaggtaat tttgcgcata agcgatctcc acgtattaac tattaataca
     7381 aagtatatgt cagaacgctt tctacaacca aaccgtattc agttgcctgt gaaacaggca
     7441 aaaaaaaacc gcagcgcaaa tacgccacgg tttttttaac agaacggatt taaaacacca
     7501 ttacggcaga attgacggct gatccgcccc ttctttttcc actttctgct gcaacatgtg
     7561 ttcgcgcttc atacccagtt tcagagccaa cgcagatgcc acatagatgg aagatgcagt
     7621 accgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg gaccaccgaa
     7681 gagatacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg tacggtgcag
     7741 cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa
     7801 gttttcacga attcggtccg ataccacgat actgtcgtta agcgagtaac cgataaccga
     7861 catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca aaatacccag
     7921 cgtaatgata acgtcgtgcg ccagcgcaat aaccacccct gccgccagtc gccactcaaa
     7981 gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca tcgcaccggt
     8041 ttgcgcaagg tctgccccca cgctcggacc gacgaactca atacgcttca ctgctgcatt
     8101 ctgattggtg gattcgttaa tcaccttcag aacctggctg cccagcacct gaccgccggt
     8161 ttcgccttca gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa agttttgcag
     8221 catcggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg
     8281 tttttcgagc gtaatttcga taaccgtacc accggtgaaa tccagccccc agttaaagcc
     8341 gcgcacgccc ataataacga tagcagcgat taacagcaga ccagagatgc caaaagccca
     8401 gtagtcccag cgcataaagt catagacttt acggccgtgg tttagttgtt caacagtata
     8461 ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg ccgccatata
     8521 gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga
     8581 taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac aggatgatga
     8641 ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca cgataacctt
     8701 catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata cgttcgttga
     8761 tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac gatacccgcg atacctggca
     8821 tactcagtgt tgcgcctggc aacagcgaca taataccgac gatcaagatc aagttggcaa
     8881 tcagagcact ggtcgcaatc agaccaaact tcttatagaa gatgatcatg aacagaatag
     8941 aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc atacccaggg
     9001 tcgggccaat tgtacgttct tcaacaatct gaatcggcgc gatcaacgca ccggcacgca
     9061 gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata cggaagctgt
     9121 tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc accagaaccg
     9181 cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg gttgccatcg
     9241 gtttgccgat gttgtccttg gtgaagttag acataatgtt gccaccagcg ctatcgagcg
     9301 agatgttaac ctgcggctgg ttgtattcgt cctggctgga agtggagtcg gtgatatggt
     9361 caccggtaag aattacgcgt ttgtacagca caaccggctg accttcgcgg gtctgtttca
     9421 cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctgatcaacg ttggtgttta
     9481 ccagacggaa ttccagcgtt gccgtcgcac ccagaatctc tttcgcacgc gcagtgtcct
     9541 gaatacctgg cagttcaaca acgatacggt cagcaccctg acgctgaacc accggctccg
     9601 ccacgccaag ttggtttaca cggttacgca ggatattgat gttctgctgc accgcatatt
     9661 cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg ctgccctgac
     9721 tgctaatcac caggtccgga tgacgcttgc tcagatacgc aatggcttca tcacgagctt
     9781 tagcatcgcg gaaagtgatg ctcaggccgt agttattttc tttacgaacg gtggtatacg
     9841 ggatgccttt ttcgcgcagg tcgctgcgca ggctatcgat attttgttcc tggagtttgc
     9901 caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc aggtcaaggc
     9961 cgagcttcat cggctcagcg tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt
    10021 taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga gcgcgcagct
    10081 gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc acagacttag
    10141 cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc tcactggcgg
    10201 cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg ttgggaagcg
    10261 catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac aaaggataac
    10321 ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc atggtgcctt
    10381 tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg tcattcagcg
    10441 cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg ccaccgttcg
    10501 tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct ttggtgcgct
    10561 tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc agcatcaaaa
    10621 tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca tcagaaataa
    10681 aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg tggtacttct
    10741 cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc ttcaatagcc
    10801 ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt attgagtcgc
    10861 gcgcctaata tttcgttgca acggtcaaga tgatgcaagt aagcgcgtga ataattgcga
    10921 caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata cttcgcattg
    10981 cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc
    11041 attacgcagt caaacatatc gataccacga cgtacgcctt caaccaggtc ttctggttta
    11101 ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcgggcatac atgctccaga
    11161 atgcggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc gtagccatca
    11221 aaaccgatat ctaccagacc tttaacagaa atatcacgta aatcttcgta aacgctgccc
    11281 tgaatgatac caaacagcgc atttttgttt ccgagactgt caaaacgctc acggctacgc
    11341 ttcgcccaac gcagagacat ctccatggag cgttttgcgt aatcccagtc agcaggatac
    11401 ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg aatctccatc
    11461 gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg ggttacggaa gtgcacgccc
    11521 tgttcggtga ttttacgaat atcgccaagg ctgaagactt ggaagccgcc ggagtcggtg
    11581 aggatcggtc ccttccactg cataaaatcg tgcagatcgc cgtgcagttt catgatttcc
    11641 tggcccgggc gcagccacag gtggaaggtg ttgccgagga taatttgcgc gccagtggct
    11701 tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg cataaaacaa
    11761 ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg
    11821 gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa
    11881 ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt gtacgtgata
    11941 aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgccgc tttataggca
    12001 ttcattgtgt gttgataacc ggcaaaggcc gaaaccagca taatcagcgt cgattccgga
    12061 aggtggaagt ttgtcaccag cgcatcgacc actttgtact ggaagcccgg atagataaag
    12121 atttgggtat cgtcaaagaa cggttcgatg agatcgtttt tcgccgcctg agccgcgctt
    12181 tccagcgaac gtactgaggt ggtgccaacc gcaatcaccc ggttaccgcg cgctttcgcc
    12241 gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg catgatgtga
    12301 tcttcaatgg tgtcgacgcg taccggctgg aaggtgcccg caccaacgtg cagggtgacg
    12361 aacgccatct ccacgccttt ggcgcgtaat ttttccagca aaggttcgtc aaaatgcaga
    12421 ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg ataaagttcg
    12481 cggtcagcgt cttcgtccgg acggtcgata tacggcggca gcggcatatg gccgatgctg
    12541 ttgagaatat ccagcaccga gcgttcatca ttaaattcga cttcaaacag tgcgccgtgg
    12601 cgcgcggtca ttgttgcgtt aatactttcg tcatcgccca gcagcagttc tgcgccaggt
    12661 tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag catccgttca
    12721 accagcactt caatcttgcc gccgctggct ttacgcccaa acaggcgcgc cgggatcacc
    12781 cgggtattat taaaaaccag aagatcgccg gggttgagct tatcgagtaa atcggtgaaa
    12841 gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt
    12901 tcaggcatcg gatagtgggc aatcagggat tcgggcaatt caaaggagaa atcggtaacg
    12961 cgcatgacac tgactcagac taaaataaga ggcgggtagt ctagtgccgg ggcgctctcc
    13021 ctgcaacctt tacccccctt tggatagaat ataatcgatg aattttttag ctcacctgca
    13081 tttagcccat ctcgcggaaa gctcgctttc cggcaatttg ctggctgatt tcgtacgcgg
    13141 aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc atcgacgtat
    13201 cgacgtattg actgacaatc tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga
    13261 aactcgccgc gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgtca
    13321 ctggtcgcag ctgtcgccgg actttccgct acaggaattt gtttgttatg cccgcgagca
    13381 agtgatgaca attttgccgg actcaccgcc acgctttatc aacctgaaca actacttatg
    13441 gtcagagcag tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg
    13501 catggcaagc cgtcgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc
    13561 ccattatgcc gccctcgaaa cccgcttctg gcagttttat ccacggatga tggcgcaggc
    13621 gtcacgcaag gcgctctgaa acttcaaact ccggcgtcac tattttttag cattcgcatc
    13681 cagcggggcg gcataacaac ggtaagaaaa acattcgctg gaatgattgg cgatatcttt
    13741 aaagttgctt tcagcaaccc cctcgaacat gagtaccgtt ctggacaact ctccgttttc
    13801 atttatatgt ggaaaatcaa attggctact gaatcgccat acatctgtct tttctggctc
    13861 gctcttcaca gagatatgtc gatggaggac ccctcctgta aacgtgcgcc agaacaattg
    13921 ttgatcaaaa tcttgtaata tcgcagactt ggatgctatt tgacgtagta aacctgcata
    13981 ccgtggactg ccattacgag caacattatg caaaccctga ctaaaagcat caaacaaaca
    14041 atagatgtga tcactagtga aaaaaggtct taattttttt tgttcttcat cgctgtaatc
    14101 caggagcatc tcattgattt cttccggtga gaattttaag acaaaattat cctcagaatt
    14161 tgtttgtt
//
LOCUS       DAZGXS010000116        13137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGXS010000116 DAZGXS010000000
VERSION     DAZGXS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            161..244
                     /gene="idlP"
                     /locus_tag="ACHKG5_003665"
     CDS             161..244
                     /gene="idlP"
                     /locus_tag="ACHKG5_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP0869681.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            241..633
                     /gene="iraD"
                     /locus_tag="ACHKG5_003666"
     CDS             241..633
                     /gene="iraD"
                     /locus_tag="ACHKG5_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0869682.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(626..1480)
                     /gene="hypT"
                     /locus_tag="ACHKG5_003667"
                     /gene_synonym="qseD"
     CDS             complement(626..1480)
                     /gene="hypT"
                     /locus_tag="ACHKG5_003667"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0869683.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(1602..2774)
                     /gene="iadA"
                     /locus_tag="ACHKG5_003668"
     CDS             complement(1602..2774)
                     /gene="iadA"
                     /locus_tag="ACHKG5_003668"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0869684.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2787..3248)
                     /gene="yjiG"
                     /locus_tag="ACHKG5_003669"
     CDS             complement(2787..3248)
                     /gene="yjiG"
                     /locus_tag="ACHKG5_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0869685.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(3245..3928)
                     /gene="yjiH"
                     /locus_tag="ACHKG5_003670"
     CDS             complement(3245..3928)
                     /gene="yjiH"
                     /locus_tag="ACHKG5_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0869686.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4178..4729
                     /gene="kptA"
                     /locus_tag="ACHKG5_003671"
     CDS             4178..4729
                     /gene="kptA"
                     /locus_tag="ACHKG5_003671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0869687.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWANIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLE"
     gene            complement(4886..10127)
                     /gene="iatD"
                     /locus_tag="ACHKG5_003672"
                     /pseudo
     CDS             complement(4886..10127)
                     /gene="iatD"
                     /locus_tag="ACHKG5_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(10338..11516)
                     /gene="yjiJ"
                     /locus_tag="ACHKG5_003673"
     CDS             complement(10338..11516)
                     /gene="yjiJ"
                     /locus_tag="ACHKG5_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0869688.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            complement(11584..12426)
                     /locus_tag="ACHKG5_003674"
     CDS             complement(11584..12426)
                     /locus_tag="ACHKG5_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0869689.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            complement(12692..>13137)
                     /locus_tag="ACHKG5_003675"
     CDS             complement(12692..>13137)
                     /locus_tag="ACHKG5_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP0869690.1"
                     /translation="CSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNND
                     NIVNQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDST
                     DKQAKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
BASE COUNT         3238 a         3180 c         3281 g         3438 t
ORIGIN      
        1 actagcggtt tttttttgag tttgatgatt tttatcaaat catgactttt ttcctgaaaa
       61 gtcagtagat attcataggc aagtaaggtt ttatactttg ctgacaggat tcaggcctgt
      121 ctcagactga catggatgta atgaacaaaa gggaatggct atggaaaatg agcatcaata
      181 cagtggtgcc cggtgttcag ggcaagccgc atatgttgct aaacgtcagg agtgcgcaaa
      241 atgatgcgcc aatcacttca ggctgtttta cctgaaattt caggcaataa aacgtcattg
      301 ctgcgtaaat cggtatgcag cgatctcctc actcttttta attctcctca ttcgacatta
      361 ccgtcattac ttgtttcagg catgcccgag tggcaagtgc ataacccgag cgacaaacat
      421 ctccagagct ggtattgccg tcagctgcgt tctgcactgt tatttcatga gccacggatt
      481 gcggcgttgc aggttaattt gaaagaagcg tactgccaca ctctggcgat cagccttgag
      541 ataatgctgt accacgacga tgaaccgcta acgtttgatc tggtctggga taatggcggt
      601 tggcgcagtg cgacgcttga gaatgtcagc taagcacaat ctccagctcg cgcagttcac
      661 gccagaagcg ttcggcaact ggattcatgc gggtattcat ccggtatgcg taggcctgaa
      721 tcgggatcac cagttcgtcc cgattcagca caacgagctg cccgctgcga atttcttgtt
      781 ggatggcata ctccggcagc caggcaatcc cacagccgtc gagggcaacc tgctttaaaa
      841 gctcgctcat cgaagagaca aaaaaggtgc tgaaacttaa ctcactgtgg cgcgtcaggg
      901 tgcgattaat caatcgcccc atgtaggagt tgcggctgta attcagtaac ggaaagtgtg
      961 gctgcacgag gtcaaaaagt gcttctccat gttcgtcact ggcgcagaca gggaacaatt
     1021 gagattcaaa taagcgaatg tggtcaaacg gcgcttccag caaatcttcg tcgtgaaagg
     1081 aaaaaataca gtcactttgt ccttcacgca gtttatcgac cgcttcatcg acatcaatgg
     1141 cttcaatcgc ccaggtaaag agcggcggca tctggctgat aatggacggt aacagaccga
     1201 gggaaagaga gtgtgcagcg gctatcttga ttttacgttg cgcgtaatcg ctgccgccac
     1261 gcagctctgc aaggttgctt tctaactgtt gcaacagatg gcggatctgc gaatggaaga
     1321 ttttcccttg ttctgagagt tgcagcggcg tcacctggcg gttaaacaat tcaacgccaa
     1381 tcgcctgttc cagcgcgcgg atgcggcggc tgaatgccgg ttgcgagacg ttacgactga
     1441 ctgccgcctg ggaaaaattg cggcattttt ccagggtcag aaaatcttaa agccatttgg
     1501 tttcaatatt atgcaaaacc gcaccacagt catccattac gttttactct gtcagccgcc
     1561 cttgggggca ttctacgtcc attagggcgg ctgacaaccg tttaagccgt ttcaaacgtt
     1621 cctttcacgc aggctttgcc gtctttgacc atcagtttgc cgcgagcgta tacctgctca
     1681 atgcgcaatt ctggcgtcat gaccagtaag tctgcgtcat tgcctggcag aatttcgcct
     1741 ttcccggtca ggttaaggaa accggctacg ctactggtga gcgggcgcag ggcatcgctg
     1801 atactgaaat catagtcttt aaccagcacc tgcacggttt ccagcaacgt ttcaaaacct
     1861 gcaacgccga tatgggttaa attcccttca tcatcgaaga acggctggct accgttgccg
     1921 tcggagctga gggtgacgcg tgccagcgga atacccgcct gaacggcgcg gacaatacct
     1981 tcggcagggg cgaccggttc gtcaatgctg ctggtgatat cgatggtgcc gcctttgcgc
     2041 gcgaactcca gcgcctgctc aaacaacggt acgttgcggt taacgtgggt cggcagcagc
     2101 ttgctgatcg gcacatcgca gttttccagc aggtcataaa taggctgtaa tgccttttta
     2161 ctgtcgccca tgtggaacac ggtgacgcca ggtttaccgc cgagcaaacc gccaacgcgg
     2221 gattctgccg ccatattagc caggtgataa acgtccggtg cggcagaacg gtgatcagag
     2281 atggcacatt tcacgccaat cacacgatcg ataatcgcca cgtctttttc cacggaaccc
     2341 gtaatggtgc gggaagggac atgataagcg ccggtcagca tccaggcgct gatgccttct
     2401 tcattgagcg cacgggtctt ggcgagcagg gattccgggt ggcgagagat agagtcggtg
     2461 cccagcagac caaccactga cgtgacgccc gcttccgtca ggcgacttag cgccacttcc
     2521 ggcgtgcgcg tcgtgggacc tgcttcgccg ccaccgccaa tcaaatggac gtgttgatca
     2581 ataaaacctg ggcagaggat ctgcccgctg agatcgacaa ccgtgcagtc cggtacaatg
     2641 tcagaaggga tattactggc aacggcgata attttgccgt tagcgacgag gacatcgcaa
     2701 attccccgat cttccggcgc atacaaatgc gctccctgca gcagggtaaa accggctgcg
     2761 gtataatcaa tcatggtaac tccttgttaa acaataagct gcataaccca gatcgacagc
     2821 aatgcgttaa tcacgcagac cgtaatgata tgcgggtaat atttggcatt cacttcggct
     2881 gtccccaggc agcgaccgac attttgcacc gggttgccca ttaaatacat cgcgggcagt
     2941 aaaacggtga catcgtgacc ggttaatgcg ccagcggttg ccagactggc ggcaacaccg
     3001 actgcgccgc ccatactcat taatgcggct aaaaggacgg ttgccgcttc accgggtaat
     3061 ccccacagcg ccattacggg ttcacagata tgccccaccc agtcgagcag gccggtaatt
     3121 ttcagcgcct gaatgatgac aaacgccatc accacgtttg gcagcaggtt ggtcgtggcg
     3181 atagtaaagc cacggcgcgc gccatcgata aacatatcca tgacgttttt gcggacctga
     3241 gttgtcatgc ttgcgctcct tgcgttgggt tgcgacgttc ttcgaagtta agccagacgc
     3301 gcaaaatgtt ggctccgaca aatttaaaca ccaggatgac ggctaaaggt acgattacgg
     3361 acgtgcccag aaaggcgaac accgcgacgc cggaagagaa gtagttggtg atgatggcgc
     3421 taccgctggt ttgatatgcg gcgaaaatca ctttatcgcg ttcggtaatt tcaccttcct
     3481 gcgccagctc tttggtcata ccggcggcgg catcagtgtt ttgcaagtta gcgatcagcg
     3541 ccagcgagca gatccctgga atgcccaaca acggttttag cacaggtgtc attaactgct
     3601 gcgcggcgcg taatccacca agaccatcgg taattgaaat aatccccaga gagagaatga
     3661 ctgacggtgc cagctccagc gcaaagagaa agccatcttt agcccccgcg ccacccgcgc
     3721 cgcgaaatga ggtggtggct ccattcgcac caggcagttg cccaaacgat ccgttcagaa
     3781 cggaaaaatc aaaaacgcgc caccagctgt ctgttccgga aaaaacaccg gaaaaaaaga
     3841 ttatggtgag aaaaaaagcc agataccctt taatgcccac tttttctgtc gccagctccc
     3901 cggctacagc atccccttgt tgtgtcataa caatccccat tgtttttttg tgtttgtgtg
     3961 ttgcttgagc caggaaaatt cctggaactt aaaaacctta tcagccatgt gggtatgggg
     4021 aaaatgcaag tatgtttctg gctatgcgtt ttttgcatag gggaaaaatt acgctgtgtg
     4081 gaaaaggttc tgcgcaggat tgagttgcag aaaattcata aacccagtga caatgacatt
     4141 ggtaaataaa aacagaaaat ccagggacga aaacgcgatg gcaaagtaca acgaaaaaga
     4201 gctggcggat acgagcaagt ttttaagttt tgtcctccga cacaaaccgg aggcgattgg
     4261 tatcgtactg gaccgtgaag gatgggcgaa tatcgataag ctcatacttt gtgcccaaaa
     4321 agcgggcaag cgattaaccc gtgcattgct ggataccgtg gtcgcaacca gcgataaaaa
     4381 gcgttttagt tattccagtg atggtaggtg catccgcgcg gttcaggggc attcgacttc
     4441 gcaggtcgct atttcctttg ccgaaaaaac accgccacag tttctctacc acggtacggc
     4501 aagccgtttc cttgacgaga taaaaaaaca ggggctgatt gcaggggatc gtcattacgt
     4561 gcatctctct gctgatgaag caacggcacg caaagtggga gcaagacacg gatcgccggt
     4621 tgtcttaacc gtcaaagcac aggaaatggc gaaacgaggc atccctttct ggcaagcgga
     4681 aaacggggtc tggctgacat caacggttgc tgttgagttt cttgagtagt cgtttatgcc
     4741 ggctggggta caaaaacaat aaattcaata tattgcaggg gttatatagg cctgataagc
     4801 gcagcacatc cggcaatgta gcgtttgtta tcagtctgaa aggataccct cgtaaaaggg
     4861 tatcctttaa taacaactgc tgaaattaaa gtgatttacg gcaggcggtc agttgggggc
     4921 tggaatcagc gtcgcttccg gtcataccgg ttagcatctg cacatagtag tgccgactgt
     4981 aatcatcaga ttcacttgtc cagtaagctc ccggtgtaaa ttgcgaatta ctggaaaacg
     5041 ctccaacgtt cccccattca ccatacagtg atccctgcgt gcgtttgttt tcgccagacg
     5101 tcaaatgtgt gatgccagga agattatatc catcattttt agcatgacag agcgcattgg
     5161 cagctagcca gatattggta tgatttccat tattcaccca ccagcctttc agcttaatta
     5221 aggtctggta ggttgtacct ccttgtcggg gaacagcgct tattgtgact gacgtttgat
     5281 cgccgatatt tgcgaaagaa actttacccg aagcatcgac agacacccaa ccctgtgaac
     5341 ttgaaaacat ataatcatca acggttttac ccgcttcaaa gttatcattg ttgagtgtgt
     5401 aatacgctcc tgcaaagcct tcgctcggga atttagccgt ggagaaggta tggttgtttg
     5461 cagtgacatg accggacatt tcttttatcg attggatgag attcagcgat atgcttaagt
     5521 ttgcctgatg aaccccgttc agcatgggaa tgagtgtcgc tgttccctct tttccgccag
     5581 tgacagtcac ggtgtactcg ccataatttc tttggctgta atcaatatcg ctcaacttga
     5641 gaaagttcac gaaggggcct gactggacaa attccagatg atcagacgtc actatcgggt
     5701 taccgtactt gtcttgcaat gttagcgtaa gttttgcgct ctgctggtag tcagccttca
     5761 gagtgtcaac atcggatttc aaaattgact tactttcatc ggccgtgccg gcaaccaatg
     5821 taatggttgc ggattggttg gctgtgccag gaacttgcac attgactgtt aaggtttctg
     5881 ccaatgtact ggttattgtc gccgtgactt gcccggactg atcggtacgc gtagttggaa
     5941 tattaactac cgggctgcct ttggtgtcta agccatgact aatatcgaca ccttcaaccg
     6001 ggaatccgta atcatcttct actcgtgaag tgatggtgct gctatcgatg ccgtcggcgg
     6061 tgatggtgtc aggagaagcc acgacgctca caacatgtgc cgttttagca tctgccttaa
     6121 ccgcaaccgt tacctggctc tgaacgggaa catctttgcc cattatggcc gtaacggtat
     6181 agacattcac tttgtcactg gtcagcgtca ctttggcctg acctgactgg tcagtaagga
     6241 cttcccctgt attgcttagc gttgcacctt caggcgttac gctaaaggtg ataatttgcc
     6301 ctggcatcaa ttcaccccgc gcatttctcg ccgttgcggt cagcgtaata ccctcactat
     6361 tatggaccgt agtttctggt ttcgatgatg ttaactcgag tttgccattt gccacattca
     6421 cgtcaacctt actactactg ttattgtcca gcgttgccgt cacctgataa accccaggtt
     6481 gggtgctgag aaggttaacg tacgcataac cgttattatc ggtgtaagcc gtatcgccta
     6541 tcgtaatgtt agttgttggc tgcgcactta atctgacgcg ttgacccgcg attacattgt
     6601 taaacgcgtc ctggacgagt atccgtagcg tgatgttttc gcccaccgtt atcgtttttt
     6661 cggctccggt tatacttatt accttcgccg aactttcatc cggtagcact gataaactaa
     6721 acgttttatt atcggaagct tgcgctttcc ggctcaccac gacgttatgt tgtccggcaa
     6781 tattgctggt cattgttacc gtcgcctgac ccagcgtatt ggtggtgact tctgtttcgc
     6841 ttagagtgac atcgttactc tggaaagtga ccaactggtc tttaagcgga ttttggtaag
     6901 cgtccaggat cgttgccgtc agggtactcc ccgtactgtt acccgcaaca atggaattta
     6961 atgaggcagt cagcggctgg agctgcgcac tgtttgtatc cccaataaaa gtgacttgct
     7021 gataagcaac ggtattattt ctggtcagcg tcgccgtaac tgtaaactca cccgcttttg
     7081 tgcctcgcaa tgtgaccacg gcatcaccgt ttgcatcggt gcgggcggtt tcactactta
     7141 gcgtgaagtt cccggtcatg gaggctggca aggtaaattt cacatcctca tccggcaacg
     7201 ggtgtccata aacatctttc acactggcag tcagggtaac gatatttgta ttgttagcga
     7261 tcgcactgcc cgacccggca ttcaaggtca ttaccgattg ttccctgttt gggaccacgg
     7321 tgatcgtgac cgactggtga atatttttat ccacctcaag cgaggcatcg acggaatagc
     7381 taccagcttt tgaactggtc aggtgcaccc gcacaatacc ggactggtct gttgtggcgg
     7441 tcgtgttgtc cagttgcgag ccaccaggtg ccacaaaatt cactttcata tcttttaacg
     7501 ggttgttatg aaagtctgtg atgatggcgg tcagagtact gccctggctg ttgccagcaa
     7561 taatggtatc tggatcggca accagttgtt tgaattttgg cgtactcgaa tcgctaacga
     7621 atttcgcaat tatccgttgt gaaaaaccat ttggcagcgt ggcgctaatc gtattttctt
     7681 ccgcaacctg acttaccatg tttattgtgg caacaccata tgcatccgta ttttgctgcg
     7741 gcggttcgac aaactccgcg cttccttttt cattcttaaa ggtgacctga tgatttgtca
     7801 ggggattatc actctcatca gtgacgttga tcgtgagggt attaaatgtc tttttatcgg
     7861 cgtaaacgtc attatttgtc gctgataatg tggcaacgcg agccttattc tggtttgctt
     7921 caatgttata aatatgcgat tgcagtggag cattccagtt gtgaaggcta agttgtgccc
     7981 ttagtgcagt cccttgctta taggccggat aattcgcggc ataaaccccc ggctgctctt
     8041 cgtgccagac aatcgtggcg ccaggtttgc tgttttcgac agtaacggct tttaccaatt
     8101 cctctttttg atatgcaacc ggttgattgc tatcgtcttt cagttccacc ctaaccttga
     8161 tgtcgtctcc ggcataatac gatacgttat caattgttat tgatgaatgg tcgcgggatg
     8221 agacaacagg gacgatatta acgacgatgg attcttttac cgcactctca ccgttaattt
     8281 gcggcgtcag tgttactgaa cctgatgtcg ttccggcggt cagtatctgt gtgtaactac
     8341 catcaccgtt atctgtccag tcagacaggg tgatatcctg aacgccttca ctgcgggttt
     8401 gcagcgccag ccccggcacc ggagttccgt cggaatcgtg cgcagtaacg gtcaatgtgg
     8461 tcgtcgattt tttatctgca gccacggtta gcggattgac ggataacaga gaatctttct
     8521 ggcttaatgt cggagcctga ataactacca tgctttgttc atgccgtgac aggttacctt
     8581 ttacatcctc ggcggtaacg tctatcgacc atgtattatc ggtttctggg gtgttagtga
     8641 agcggtaacc tggcagcgtg accgtgatat cttttccaga cgtgctgact ttacctccgg
     8701 cagcttccag cgcagcggct tcgatgttat agcctttaag ggcatatttg gtctgtagcg
     8761 aggaaaccag cggttttatt tctccagact tccctttcac cggatccagc agactcaggc
     8821 gaatcagctc tttcttgcgg tattccagaa cgatgttgtt gttgcgatca atcaggtcat
     8881 aacgactacc ggccagactg cgccgtccgg ccacttcgtc cggattaagc tgtttctgca
     8941 ttgaactgct gggttgccag gtcagatcaa cggcaaaacg tgtgtcattt tcaccttgct
     9001 tcccctgacg ctgttccgca ctgagagtca gaagcgggaa gggggtatag ttgaggcctg
     9061 cggtaatagc atgggggtta ctttgacgat cattcttgtc aaacagcgcc acttcatcgc
     9121 catagtattg ttcatagacc agttttcccc ccagttgtgg ccaggcaggt aaccagcctt
     9181 ccgcgcgtaa atcccagccg ttggccgggc gggcttcgaa gtcgttatcc aattctggtg
     9241 cgctacgcca gccggtcagg ccgatataag cgttgctgct caacttcaga taatcacgcc
     9301 agtattctgc gccaagccct gcgcgggagt gatagcggct caggtcgtgg tcaaaaaaaa
     9361 ggttgatgcc tgacatccag ctggaggtga aatgacgcca gcccaaaccg gtgttgatct
     9421 gggtacgatc gtctgttcgg tgaagggtat gctggctgaa gagcagataa tcaggtgtgt
     9481 cataccacgg atgcaggaag tcgaattgcg aatttttcag gctaaaatct tcatccacac
     9541 ccagagagat tctcgcagta ccaaagttat ttaaccaatc agtcatcgcg cctgaggctt
     9601 ctgaagacgc ccaaccacgc gccatgccgc tggcctgctc actattcata tcttgtgaaa
     9661 gcagagtgcc aactcgctgc gaggtgctgg ctatttgatt ctccagcgtg ttttcgccat
     9721 tcgcaggcgg tacggcattt tgttgctcat ggcttttctg cgaggttgtt gccggaacat
     9781 ccagttcttc accctggcgc acgttatcaa agccgcgagc aaaagtacgg aactgattaa
     9841 gacgacgaag ctcctccagt gaaataccga agcgattagc aacgctttgc gctgattcca
     9901 gcgcaccaag cgtgtaaggc acagtattat tttctagtag aggggggggc agatgaaact
     9961 gtcgtctgag gtttagcatg taccacattt tgtgccgtcg ccatgacggg gaaaaaaaat
    10021 tgtgtaaata aacaaactcc ggcggtcagg cggcgtattt tgtcacccag accacataaa
    10081 gagttttgat cattattttt tatttgatgc gcatttgtcg aagccatagt gaccttccgt
    10141 gaaaaaacag aattaaactt gttttttcta gcacggagat ataatgcgat cctgactata
    10201 atatgaccag aaataatgaa ttagccgtgg aattgctaaa ccaggaataa taccggcggt
    10261 ggctgagtac ctccaaagaa aaaggatatt ttaattattt tacgtgatgt gctgaaaagt
    10321 taaattagtt aaattactta agaataattc gtatgcaatt tgaatatttg catcccgcag
    10381 attaatgctg cgataaatag cgccgctgcg ccgcagagtg tggcgctggc gagcgccgac
    10441 gttccgttgc cgagcatact ggtcagcgcc gggccaagaa tttgcccgat accataaatg
    10501 agtgtcacaa agcccaaaag gttaagattt cccggcacgc taagctggcg ggcgatggtc
    10561 atcaccagcg aggtcgttcc cataaaggtg ccgccaaaac caatactgct gatgatgagt
    10621 aataaaggag agctgctggc gagggttaac agcacgcaga tcgcctgcac cagcaaattc
    10681 gcggtcaggc aaggtaatgc tccccaccgt ttggctgccc acagccagcc aaagcaacct
    10741 gggacaatcg acaagccaac cagtgtccag aggtgagccg tcaacacagg ctggcccgcg
    10801 tctttcgcca tgagcggcag gtaggtggcg acgatgatat aaccaaaacc cgccagacca
    10861 tacagaatcg ccagtaacca ccagctcatg ggttgttgcg cgatttttgc caatggcgct
    10921 ggcgggataa cgtgtttatt cgacgggatg agcagcgcca gagcaagcaa tataatggca
    10981 gaaagtgctc cggcaccttg ccacaacgtt tgtgaagaga gggcaaaatg caggcctgcc
    11041 agcacatatt cattacccag agcgatgccg acgccaacac cagaaaatag cgccgcaagg
    11101 acaaagggat ggcgagtatg ttgcatgatg agtgttgagc cgaaaatcaa catcccggcg
    11161 ctggcgactc ccgccagaaa gcgaatgatg aaaaccagaa gaaacggcgg cagccacgcc
    11221 atcgcgagga ttaataatcc ggttgcgagg gcggaagcta acaggaacgg gcgcaggcgt
    11281 gagggtaaat gaaacgcgcc gaatgaaaac agcaggctcc ccgccagata cccggcatag
    11341 ttaccactgg cgatccatga gagttcgcta aatgaaaact cgccttccgc cagcatgact
    11401 ggcagcatcg gcgtatagag aaagcggcct aaacccatac ctaaggtcag aaccagcatc
    11461 ccgaataacg cggtgctgaa agaaaaacgt tctacgggat gcgatgtcga attcatgttc
    11521 tgcctggttt gtattatttt gagttaacaa gttaataaca aaaaaggaga acctgccaga
    11581 ctgttatcgt gattttgtcg acgtaaagcg ataaaaaaga ttcggttcgc caacgatata
    11641 gatgttgccg ctatcatcca tcgctatccc ttccgcctgc ttgatgttgt gtgaaaggcc
    11701 atacttacct ttggttaacg acatttcgcc gatcacgtca cctctcacgg tcacttcctg
    11761 cagggcgcgt gactcatgtg acaacaccag caacgtattt ttctgtgggt taaattccgc
    11821 gccagaaaca tcgtctaaag taaactgacg ctgcaacgtt ttatctttgc tgatatgcaa
    11881 ctcatcgcta cgcaatagcc cggttacttt gtagacctct atcggatttt tctctttaaa
    11941 aaaccagaag gtatggtcct gagaggagta agccagccct tcaaagccac agttagttgg
    12001 tgtttcctgc aatgggattt taattttttt gaggatgctc acttccgagt cagcgttaag
    12061 tgaaatcaca taaatggcgt agtcacgttc atcactgatg acaaatttat tatcaccaat
    12121 gtattcaatc gtctcgagat ccttaacaaa atcgagcgga atggtgcgga tcaagtcgcc
    12181 ctcggtcgtc atttcaacga tggttgcagg tttgttgata gtgctaaata aggtattgct
    12241 ctgcgctgac caggtcagtg aggagatatt tttagttact cctgtaatct ctttaccatc
    12301 gatagtggcc tgataattct gaaaccctgc gacatgctgg cttttacgga cgcaggattg
    12361 cacaaaaaac aggcttgccg ccacgatggc gcaaagaaaa acgataaagg tgatgcgctt
    12421 tttcaactta ttgctcctta ttattgcttt tttctcgtca aattcagtgc gacagataag
    12481 gagtatacgg atgaagctta atgaattctt aacgcgggcg atgaaatagt aaacgccgca
    12541 gggggcggcg gttgtctctg gatggcagaa cattttcagc ttacatcgac tgataaatgc
    12601 gcctcatcta tgaatgatag atgaggcggt taacgtttaa atgttaatct atatcgtctg
    12661 ccatacccac tctgtttggg tgattaatga actattcttc aactatatat gttggctgat
    12721 aagacgttaa acccggaatg gaaaatctcc tattcacttc tttctttaaa cgggcttgat
    12781 taaaactgtt actgtcctgt tgctcggtac tgttcttggc ttgtttatcg gtactgtcat
    12841 tgacctgttt atcgatattg tcattggctt gtttatcggt actgtcattg gcttgttgca
    12901 ccgtactgtc aatggattgt tgcaccgtac tgtcattggc ctgtttatcg gtactgtcaa
    12961 tggattgttt ctcggtactg ttattggctt gattcacgat attatcattg ttcagatatg
    13021 ctccctcaag atagacattt ttcgctctga cattgtaaaa acaggtattt ttaaaaagcg
    13081 tttttataaa tattgtattg tcaaaccatg cgtagtcaaa taggatattc gaacaat
//
LOCUS       DAZGXS010000117        13132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGXS010000117 DAZGXS010000000
VERSION     DAZGXS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1067)
                     /locus_tag="ACHKG5_003676"
     CDS             complement(<1..1067)
                     /locus_tag="ACHKG5_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFP0869691.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECG"
     gene            complement(1158..1242)
                     /locus_tag="ACHKG5_003677"
     tRNA            complement(1158..1242)
                     /locus_tag="ACHKG5_003677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Leu,seq:caa)
     gene            1438..2457
                     /gene="ahr"
                     /locus_tag="ACHKG5_003678"
     CDS             1438..2457
                     /gene="ahr"
                     /locus_tag="ACHKG5_003678"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0869692.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2587..4089
                     /gene="yjgR"
                     /locus_tag="ACHKG5_003679"
     CDS             2587..4089
                     /gene="yjgR"
                     /locus_tag="ACHKG5_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0869693.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4250..5332)
                     /gene="lptG"
                     /locus_tag="ACHKG5_003680"
     CDS             complement(4250..5332)
                     /gene="lptG"
                     /locus_tag="ACHKG5_003680"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0869694.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5332..6432)
                     /gene="lptF"
                     /locus_tag="ACHKG5_003681"
     CDS             complement(5332..6432)
                     /gene="lptF"
                     /locus_tag="ACHKG5_003681"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0869695.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6495..6545)
                     /gene="ytgA"
                     /locus_tag="ACHKG5_003682"
     CDS             complement(6495..6545)
                     /gene="ytgA"
                     /locus_tag="ACHKG5_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0869696.1"
                     /translation="MSIIILATISTTQENK"
     gene            6699..8210
                     /gene="pepA"
                     /locus_tag="ACHKG5_003683"
     CDS             6699..8210
                     /gene="pepA"
                     /locus_tag="ACHKG5_003683"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0869697.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8564..9007
                     /gene="holC"
                     /locus_tag="ACHKG5_003684"
     CDS             8564..9007
                     /gene="holC"
                     /locus_tag="ACHKG5_003684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0869698.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9007..11862
                     /gene="valS"
                     /locus_tag="ACHKG5_003685"
     CDS             9007..11862
                     /gene="valS"
                     /locus_tag="ACHKG5_003685"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0869699.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11917..13113)
                     /gene="yjgN"
                     /locus_tag="ACHKG5_003686"
     CDS             complement(11917..13113)
                     /gene="yjgN"
                     /locus_tag="ACHKG5_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP0869700.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         3379 a         3360 c         3446 g         2947 t
ORIGIN      
        1 tcgccacatt ctgccgcttt ggcgattaac tcatccagcg tcttaacccc gtgcttacct
       61 ttcatcaaat tacggttagc aggaattgtg cgcaacttgc tctcataagc ccggaggaaa
      121 tcacggtcga aatcttccaa ctgaatatcc cccatcactt caatcatcaa atcgaagtaa
      181 tagcgaatat cgcggagcgc cgcttctgat accttgccct tacgtcgatg tttcaaatac
      241 tcttcacgaa gccatgacaa gttttcattg taatgtttgc ggtgaacata ttcattagcg
      301 ataacaggag cgacaagagg cacggcaggg tgaatgctaa ctgctggtgg cgtggtctta
      361 accagccatg taagtcgaag gccttcagca tgaactttgc taatcaacag gctatcagca
      421 gttatatcta cgccgggtaa atcgaagaac caacctccac tttggttatc accactagtt
      481 cgaaatgcag cctctccttc ggatactcca gcgctcaaca gacaatctaa agtatgccgg
      541 ggattgaagg ggcggagata accatttgcg aagctctcga tacccagctc aaacgcgcta
      601 ttgagaatga tgacttcttt aggttgatcc ttttcatcat cagactgttt ttctcgtttt
      661 gcacccggtt cccgctctac tgcattaaat tcatcaacat agaaaccgaa ctgaccagtc
      721 gccagccaga ataccgggag gttacgatta accacctctc gaatcaattg cgatataggc
      781 aactcgattg actgagcaaa gttagaaagc gagatcatac cctcaccagc aagcagtggg
      841 gcgcagtcac ttaaagttcg ataatccaat tgttcatact cttgtaaggt ctgataccag
      901 accgccaaca ggagcgcgga cttctgcttt gcttcatgaa gatcgtgagt gcctgttgaa
      961 gtgtgaatct cacactgtcc tgcgtatcga cgaaaacgtt ggggaacgct aatgcggaca
     1021 acataaatgc cgctggttcg acgatagagt ttatatcgct gagacatcgc ctcgtatacc
     1081 cttttgtaag aggggctacg cgatttcaag caagattatg aattaaattg ttttaaaaca
     1141 atagtttaga tgagtcttgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt
     1201 tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga
     1261 aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc
     1321 gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt cacactccag
     1381 gcaccccgcc ctgccatgct ctacacttcc caaacaacac cagagaagga ccaaaaaatg
     1441 tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac
     1501 gatcccggtg agctgaggcc acaagatgtt gaagtgcagg tggattattg cgggatctgc
     1561 cattccgatc tatcgatgat cgacaatgaa tggggatttt cacaatatcc gctggttgcc
     1621 gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga taaaggtttg
     1681 caggtcggcc agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc
     1741 tgtattagcg gtaatcagat caactgcgag caaggtgctg tgccgacgat tatgaatcgc
     1801 ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat
     1861 attgatatcg agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg
     1921 ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg
     1981 catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat
     2041 ccggcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat
     2101 ccgcaggcac tgaaagccct gtcggggcag tttgatctca ttatcaatac tgtgaacgtc
     2161 agcctcgact ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc
     2221 ggtgcggttc tcacgccgtt gccggttccg gcctttacgc tgattgcggg cgatcgcagt
     2281 gtctctggct ctgctaccgg tacaccttat gagttgcgta agctgatgcg ctttgccgcc
     2341 cgcagcaagg ttgcgccgac taccgaattg ttcccgatgt cgaaaattaa cgatgccatc
     2401 aagcatgtgc gcgacggtaa ggcgcgttac cgcgtggttc tgaaagctga cttctgatct
     2461 ctttgccggg tgcctgtcgc ccggcactta aatctctttg gaaggcgtct cgcaatctgc
     2521 tgaatttagc ggtgaagagt gcgcagccgt tgcctatact ccaggcagga aactggagga
     2581 tatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact atttttactg
     2641 ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg taaaactgtc
     2701 acgctgcaaa aactggcgga gtcattgtcg gaaatcggcg tgccggtgtt tatggctgat
     2761 gtgaaaggcg atctgaccgg tatcgcgcag gcaggaacgg cgtcggaaaa actgctcgca
     2821 aggcttaaaa atatcgacgt caatgactgg caaccgcatg ctaatccggt ggtggtgtgg
     2881 gatatctttg gcgagaaagg ccattcggta cgggcgacgg tttcagacct ggggccactg
     2941 ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa tatcatcttc
     3001 cgcattgctg acgatcaggg gctgttactg ctcgacttta aagatctgcg ggcgattacc
     3061 cagtacatcg gcgataacgc caaatctttc cagaatcagt acggaaatat cagtagcgca
     3121 tcggttggtg ccatccagcg cgggctgttg tcgctggaac agcaaggcgc agcacacttc
     3181 tttggtgagc cgatgctgga tatcaaagac tggatgcgca ccgatgccaa cggtaaaggc
     3241 gttatcaata tcctcagcgc cgagaaactt tatcagatgc cgaaactgta cgccgccagc
     3301 ctgctgtgga tgctctcaga gttgtatgaa caattgccgg aagcgggcga tctggagaaa
     3361 ccaaaactgg tgtttttctt cgacgaagca catctgctgt ttaacgatgc accgcaggta
     3421 ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt gggcgtctgg
     3481 ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcggccagct cggtaatcgc
     3541 gttcagcacg ctttgcgggc ttttacgccc aaagatcaga aagcagtaaa agctgcggcg
     3601 caaaccatgc gggccaatcc ggcgtttgat accgaaaagg cgattcagga actgggcacc
     3661 ggcgaggcgt tgatctcttt tctggatgcg aaaggaagcc cttctgtggt ggagcgtgcg
     3721 atggtgatcg cgccttgttc gcggatgggg ccggtgacgg aagatgagcg taatggcttg
     3781 attaatcact ctccggtgta tggcaaatat gaggatgagg tggaccggga atccgcctat
     3841 gagatgttgc aaaaaggctt tcaggccagt accgagcagc aaaataatcc ccccgcgaaa
     3901 gggaaagagg tagcggtgga tgacggcatt cttggtggat tgaaggatat tttgttcggc
     3961 actaccggac cacgcggcgg gaagaaagat ggtgtggtgc aaacaatggc caaaagcgcc
     4021 gctcgccaag tgacgaatca gattgtacgc gggatgttgg ggagtttgct gggggggaga
     4081 agaaggtaag tcaggcgaga tttgtctttg cctgatgcga cgctggcgcg tcttatcatg
     4141 cccaccccac tgcaatatat tgaattttaa ttatttttca ggaatgaacg aagcacactt
     4201 tgctaacaat aataaaggga gctttcgctc cctttattcg ttcattcggt tacgattttc
     4261 tcattaacag ccacaggctg attaagaaga agctggcgct tggcaacagt gcgccgatga
     4321 tcggcgggat gccataaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga
     4381 caaaaccgaa actgataccg gtgaccacac gcacgcccat cggtacgcta cgcagtgggc
     4441 caaagatgaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt
     4501 tgctccacat gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca
     4561 catagttgtg caaaccgctg atagagagtg catccgggtc cagcgccacc acgcccagtt
     4621 tgtccggcgt gaggttggtt ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt
     4681 tcggattggt cagatcagat tcatcaacct gcgacaaacg ccagacttta tgttccgggt
     4741 caaacttcgc agtagcggca tagcgtacgg attgcagacg acgattctcg ttaaaggcat
     4801 aaatgctgat gccacctaac tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt
     4861 tgccatcttt cgcccataag ccttgctggg tagagagcaa cgagccgccg tacatcgcct
     4921 gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc gacccattcg ccaatcgcca
     4981 tcgtcagcaa gaccagcgga atggcggttt tcatcaccga cagcgccacc tgcatacggg
     5041 taaaaccaga agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa
     5101 gcaacgcccc aagcagagcc gccatcggga agaatatttg cacatctttc ggcacgctca
     5161 gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcaact
     5221 gatcgacaaa cttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg
     5281 tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc
     5341 tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag
     5401 aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg tcgggtccag
     5461 cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag
     5521 cagcatggct ggcagcatcg acagtacgcg tccctgacgt gggttaacca cgctcagcgg
     5581 tacgaccata agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc
     5641 tgcgcgagca cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc
     5701 gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaaatccgt
     5761 aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac
     5821 gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg
     5881 acgtgcatta ccttttggtc gaatttgcgc gaggaacaca tctttgaaat cgctgccgtc
     5941 aacgctttcg atgaacagca ccgaactgcc attagtcgct tgctggaatt gcccttgcgc
     6001 cagcgccgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga
     6061 tgaccacggt cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag
     6121 gatcattgcc gctttcacca gaaccgcttt gctcaggccg caggcatgca ttaccgtaat
     6181 ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg
     6241 caggataagc tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc
     6301 cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat
     6361 caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct
     6421 tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt
     6481 tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata
     6541 ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga
     6601 ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta
     6661 gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg
     6721 gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc
     6781 tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac
     6841 gtcggggcga actggaagga aaaccggggc agacattgtt gctgcaccat gttccgaatg
     6901 tactttccga gcgaattctc cttattggtt gcggcaaaga acgtgagctg gatgagcgtc
     6961 agtacaagca ggttattcag aaaaccatta atacgctgaa tgatactggc tcaatggaag
     7021 cggtctgctt tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc
     7081 aggctgtcga gacggcaaaa gagacgctct acagtttcga tcagctgaaa acgaacaaga
     7141 gcgaaccgcg tcgtccgctg cgtaagatgg tgttcaacgt gccgacccgc cgtgaactga
     7201 ccagcggtga gcgcgcgatc cagcacggtc tggcgattgc cgccgggatt aaagccgcga
     7261 aagatctcgg taatatgccg ccgaatatct gtaacgccgc ttacctcgct tcacaagcgc
     7321 gccagctggc tgacagctac agcaagaatg ttatcacccg cgttatcggc gaacagcaga
     7381 tgaaagagct ggggatgcat tcctatctgg cggtcggtca gggttcgcaa aacgaatcgc
     7441 tgatgtcggt gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg
     7501 tgggtaaagg tttaaccttc gactccggcg gtatctcgat caagccttca gaaggcatgg
     7561 atgagatgaa gtacgatatg tgcggtgcgg cagcggttta cggcgtgatg cggatggtcg
     7621 cggagctaca actgccgatt aacgttatcg gcgtgttggc aggctgcgaa aacatgcctg
     7681 gcggacgagc ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag
     7741 tgctgaatac cgacgctgaa ggccgcctgg tactgtgcga cgtgttaact tacgttgagc
     7801 gttttgagcc ggaagcggtg attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc
     7861 tgggtcatca cattaccggt ctgatggcga accataatcc gctggcccat gaactgattg
     7921 ccgcgtctga acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg
     7981 aacaactgga gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga
     8041 ttaccgcagg ttgcttcctg tcacgcttta cccgtaagta caactgggcg cacctggata
     8101 ttgcaggaac cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt
     8161 tgctggcaca gttcctgttg aaccgcgctg ggtttaacgg cgaagagtaa ttgcgtcagg
     8221 caaggctgtt attgccggat gcggcgtgaa cgccttatcc gacctacaca gcactgaact
     8281 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaat gctgttattg tcggatgcgg
     8341 catgaacgcc ttatccgacc tacacagcac cgaactcgta ggcctgataa gacgcggcag
     8401 cgtcgcatca ggcaatgctg ttattgtcgg atgcggcgtg agcgccttat ccgacctaca
     8461 cacagcactg aactcgtagg cctgataaga cacaacagcg tcgcatcagg cgctgcggtg
     8521 tatacctgat gcgtatttaa atccaccaca agaagcccca tatatgaaaa acgcgacgtt
     8581 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc aactggtgtg
     8641 tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgtg ctcatcgcct gtgaagatga
     8701 aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc
     8761 gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg cctggccgca
     8821 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     8881 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     8941 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     9001 gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta cgagcactgg
     9061 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     9121 atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
     9181 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
     9241 actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
     9301 ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg ggaatggaaa
     9361 gcggaatccg gcggcacgat tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg
     9421 gaacgtgaac gcttcacgat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
     9481 cgtctatata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg ggacccgaaa
     9541 ctgcgcaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
     9601 cacatccgct atccgctggc tgacggtgcg aaaaccgcag acggtaaaga ttatctggtg
     9661 gtcgcgacta cccgtccgga aaccctgctg ggcgatactg gcgtagccgt taacccggaa
     9721 gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt taaccgtcgt
     9781 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaaaatc
     9841 actccggcgc acgactttaa cgactatgaa gtgggtaaac gtcacgcgct gccgatgatc
     9901 aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga taccaaaggt
     9961 aacgaatctg acgtttattc cagcgaaatc ccggcagagt tccagaaact cgagcgtttt
    10021 gctgcacgta aagcggtcgt tgctgcggtt gacgcactcg gcctgctgga agaaattaaa
    10081 ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tagttatcga accaatgctg
    10141 accgaccagt ggtacgtacg tgccgatgta ctggcgaagc cggcagttga agcggttgag
    10201 aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc ctggatgcgc
    10261 gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat cccggcatgg
    10321 tacgacgaag cgggtaacgt ttatgttggc cgcaacgaag aagaagtgcg caaggaaaat
    10381 aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac ctggttctct
    10441 tctgcgctgt ggaccttctc taccctaggc tggccggaaa acaccgacgc actgcgccag
    10501 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
    10561 atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
    10621 cacaccgttt acatgaccgg tctgattcgt gacgacgaag gccagaagat gtccaaatcc
    10681 aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    10741 gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    10801 gagaagcagt tcccgaacgg tattgagccg cacggcaccg atgccctgcg cttcaccctg
    10861 gcggcactgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct ggaaggctac
    10921 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacggaaggt
    10981 caggattgcg gcttcaacgg cggcgaaatg acgctgtcgc tggcggaccg ctggattctg
    11041 gcggagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    11101 atcgccgcag gcattctgta tgagtttacc tggaaccagt tctgtgactg gtatctcgag
    11161 ctgaccaagc cggtaatgaa cggtggcacc gaagcagaac tgcgcggtac tcgccatacg
    11221 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    11281 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    11341 cagccgttcc cgcagtacga tgcatctcag gttgatgaag ccgcactggc cgacaccgaa
    11401 tggctgaaac aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa catcgcgccg
    11461 ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat
    11521 gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    11581 gatgacaaag gtccggtttc cgttacgaag atcatcgacg gcgcagagct gctgatcccg
    11641 atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga agtggcgaag
    11701 atcgaaggcg aaatcagccg tatcgagaac aagctggcga atgaaggctt tgtcgcccgc
    11761 gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc ggaagcgaaa
    11821 gcgaagctga ttgaacagca ggctgttatc gccgcgctgt aatcaaaaca cagtgatgaa
    11881 aacgaaggcc ggagcatgct ccggcctttt tatctcttac agaaatggta aagaaggcat
    11941 aattccccgt gaaatactgg ctaagaagcc tttatctggt tgttcttctt tattgattaa
    12001 aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt caatcatcca
    12061 tattttcagc agtggataag ccagaccgcc cgtaatcccg gatatcacca ccaacgcaca
    12121 catgcgataa agcataccgt ggtatgttaa agttgagcga aaacgaatac gcccatcatt
    12181 cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac tggtgctaac
    12241 agcaatccca aaataataga ttaactgcgc gattatcatt ttacgttgca tttccataaa
    12301 ttgctgtaaa ttctcaatat catcatttgc atacacagaa ctatcatacg catttaatat
    12361 ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta aaaatgcgag
    12421 gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat gtataccgaa
    12481 actggtattg ctccagagaa aactcattac caggctatat aaagtaccat taaaaatacc
    12541 aatggaaaca ataccaacta ttgccatcag aaccacagat acaataacac tacttgaact
    12601 attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa tggccattaa
    12661 aatcggcaag aaaaaggtta cccaccagac ccctttcata gagcaattaa aactaaatct
    12721 tacgccgttg agactggtca tcaaggcctg ataacgcagt ccttttgctg ccataaatat
    12781 aagcaaaacc aacagtgaca aagtcaaaac acagccaata agcggcatat ctgctgacac
    12841 tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaaaaa aaacattccc
    12901 accagtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat agagataacg
    12961 cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg ttaacaacac
    13021 attcaccaca caaataagaa aatatctctc acctgtacca tgaaaaacaa acgaatggga
    13081 cggaacatcc atttcattaa taacttgagc cataaaaatt ccctgaatta aa
//
LOCUS       DAZGXS010000118        12783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGXS010000118 DAZGXS010000000
VERSION     DAZGXS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            98..502
                     /gene="yaaI"
                     /locus_tag="ACHKG5_003687"
     CDS             98..502
                     /gene="yaaI"
                     /locus_tag="ACHKG5_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0869701.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            528..1241
                     /gene="msyB"
                     /locus_tag="ACHKG5_003688"
     CDS             528..1241
                     /gene="msyB"
                     /locus_tag="ACHKG5_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0869702.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1390..1956
                     /gene="satP"
                     /locus_tag="ACHKG5_003689"
     CDS             1390..1956
                     /gene="satP"
                     /locus_tag="ACHKG5_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0869703.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1991..2578)
                     /gene="mog"
                     /locus_tag="ACHKG5_003690"
     CDS             complement(1991..2578)
                     /gene="mog"
                     /locus_tag="ACHKG5_003690"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0869704.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2693..3646)
                     /gene="tal"
                     /locus_tag="ACHKG5_003691"
     CDS             complement(2693..3646)
                     /gene="tal"
                     /locus_tag="ACHKG5_003691"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0869705.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3925..5355
                     /gene="yaaJ"
                     /locus_tag="ACHKG5_003692"
     CDS             3925..5355
                     /gene="yaaJ"
                     /locus_tag="ACHKG5_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0869706.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            5425..6201
                     /gene="yaaA"
                     /locus_tag="ACHKG5_003693"
     CDS             5425..6201
                     /gene="yaaA"
                     /locus_tag="ACHKG5_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0869707.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6354..6650)
                     /gene="yaaX"
                     /locus_tag="ACHKG5_003694"
     CDS             complement(6354..6650)
                     /gene="yaaX"
                     /locus_tag="ACHKG5_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0869708.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(6864..8150)
                     /gene="thrC"
                     /locus_tag="ACHKG5_003695"
     CDS             complement(6864..8150)
                     /gene="thrC"
                     /locus_tag="ACHKG5_003695"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0869709.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(8151..9083)
                     /gene="thrB"
                     /locus_tag="ACHKG5_003696"
     CDS             complement(8151..9083)
                     /gene="thrB"
                     /locus_tag="ACHKG5_003696"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0869710.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9085..11547)
                     /gene="thrA"
                     /locus_tag="ACHKG5_003697"
     CDS             complement(9085..11547)
                     /gene="thrA"
                     /locus_tag="ACHKG5_003697"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0869711.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11573..11686)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11628..11711)
                     /locus_tag="ACHKG5_003698"
     CDS             complement(11628..11711)
                     /locus_tag="ACHKG5_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0869712.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(11925..12611)
                     /gene="yjtD"
                     /locus_tag="ACHKG5_003699"
     CDS             complement(11925..12611)
                     /gene="yjtD"
                     /locus_tag="ACHKG5_003699"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0869713.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3019 a         3384 c         3167 g         3213 t
ORIGIN      
        1 taataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa actagtgggt
       61 attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc
      121 cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact
      181 cctcggcgtc attgcaatgc cgcgtaacga aaccaacgct ctggcgctga aacttcctgt
      241 ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagcgg
      301 tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata ttccttcaga
      361 aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg acaataaacg
      421 ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct
      481 gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg aatgttaatt
      541 acctgaatga ttcagatctg gattttctcc agcattgtag tgaggaacag ttagcaaatt
      601 tcgcccggct gctcacccat aatgaaaaag gcaaaactcg cctctccagc gtactgatgc
      661 gcaacgaact gtttaaatcg atggaaggac atcccgagca acatcgccgc aactggcagc
      721 tgattgctgg agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac
      781 acggtaaatt gtatcgggcc attttgctcg atgtttcaaa acgattgaag ctgaaagccg
      841 acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt ctgcgtaata
      901 cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg
      961 tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg
     1021 tgtcgcatct gctttccagc caactgaccc gcattttacg cacccacgca gcaatgagcg
     1081 tacttgggca tggtttgctg cgcggcgcgg ggctgggagg ccctgtaggc gcggcactaa
     1141 atggggttaa agcggtcagc ggcagctcct atcgcgtgac gattccagcc gtactgcaaa
     1201 tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa
     1261 ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg
     1321 ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga tgatgatttt
     1381 tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc
     1441 ggcatgacca ccattctgct taacctgcac aacgtaggtt atttcgctct ggacggtatt
     1501 attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg tctgctggag
     1561 tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggctg
     1621 acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag
     1681 ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcacg
     1741 ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg
     1801 ctggcgatcg gtaacattgc cggtaacgcc gcaatcatcc actttgccgg ctggattggt
     1861 ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtactgaa cgagcagttt
     1921 ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc ccccgctcgg
     1981 ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca
     2041 cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
     2101 caaaaatacc gtgtaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
     2161 taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcacaccca
     2221 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
     2281 caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
     2341 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
     2401 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
     2461 acggcgtggt tagcgccgat gtcagccatt cttccagcgc agggatacct ttatcctgat
     2521 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
     2581 agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag
     2641 acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca
     2701 gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg
     2761 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
     2821 tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt
     2881 cgctctcagc cagctctttc agcagtgccg gtgcgatggt cagacggtcg cagcctgcca
     2941 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt
     3001 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     3061 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     3121 agatcaggaa cacgcccgct tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     3181 tcaggttaca gttgatacct tctttttcca gctgttccgc agcacggata ccctgccagg
     3241 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
     3301 tcaggcgttt tgctttcgca attgacgctt ctgtgtcata ggaaagacgc gcatcaactt
     3361 cagttgagat acggcccgga accagtttca ggatttccag accaatattt actgccagtt
     3421 tgtcagtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
     3481 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
     3541 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
     3601 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
     3661 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
     3721 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
     3781 cgttgatatc gcccgccatt tttaaacaaa acctcatgta tgctacgcag aagttatcaa
     3841 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
     3901 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
     3961 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
     4021 ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat
     4081 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg
     4141 ggtagcggca acctggccgg cgttgcgctg gctattaccg ccggtggacc tggagccgtc
     4201 ttttggatgt gggttgccgc gtttatcggc atggcgacct cgtttgccga atgttccctt
     4261 gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat
     4321 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
     4381 gcctacggca taattttcag cggagttcag gcaaacgctg ttgcccgagc tctgagtttt
     4441 tcttttgatt ttcccccgct ggtgacaggc attattcttg ctgtctttgc tctgctggca
     4501 atcattcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg
     4561 ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac
     4621 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg
     4681 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
     4741 gcgggaatgg ggtcgacgcc aaacgcggca gcggcagcgg cgtcctggcc tccgcatccg
     4801 gcagcgcaag gaattgtcca gatgattggc atttttatcg acaccctggt catctgtacg
     4861 gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt
     4921 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc tgaatttgtt
     4981 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     5041 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc
     5101 accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg gcaactggca
     5161 gatatcataa tggcctgcat ggctattacc aatttgactg ccatattact gctctcgcct
     5221 gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg
     5281 tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctcgggacgc gtgggatgat
     5341 gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc
     5401 ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat
     5461 taccaaagcc cgttgaccac cacgcgatat acactgccgg agctgttaga caattcccag
     5521 cagttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgtatc
     5581 agcgacaaac tggcaggtat caacgccgct cgctttcatg actggcagcc agatttcacg
     5641 ccggagaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac cggcttgcag
     5701 gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc
     5761 ggcttgtatg gcgtactccg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg
     5821 atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc
     5881 aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca
     5941 gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg
     6001 ttcctcgatg agaagaacgg caaatttaag ataatcagct tctacgctaa gaaagcacgc
     6061 ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt
     6121 tttaatagcg aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt
     6181 aaacgctacg agcagcgtta agtttgtagt caatgccgga tgcggcgtga acgccttatc
     6241 cggcctacaa aagcatgcag attcaatata ttgcagaatt cacgtaggcc tgataagacg
     6301 cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg
     6361 tgatgtttgc ctggaccatg accgccgtga tgatcatgat gagctttctt atggtgacgc
     6421 ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc
     6481 caccagccgt ggtcgcgcca gtgaccgcca tcccagtaat aaccgcgatt atcacgatcg
     6541 cctatctgta attttactga cgggactaac gtaatttccg cagcctgcgt tgccatggga
     6601 gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc
     6661 ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagag cggcggttgt
     6721 tattctccgc aatcctaatc tctaagttac cgcatttatt gagaatttct cctttttccc
     6781 tgtcgtttct ctccatattt tcttcacaaa aaagccgggc tgcataaaag caaacccggc
     6841 ctgattgaga taatgaatag atgttactga tgattcatca tcaatttacg caacgcagca
     6901 aaatcggcgg gcagattatg cgaaagcaag ggtaaatcgg cacgttctgc cagctctttt
     6961 ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc
     7021 gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag cgcacgatac
     7081 gctacggcag cgtgcggctc cgaagtgtag cccagttctt ttaactcacg cattgtctgt
     7141 tgcgtggttt catcatccac ggcggcataa cccagctctt tcagttgcca gattttgcgg
     7201 cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat
     7261 aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcat gcaggaaacg tggcacggta
     7321 tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgagtga cttcgccagc
     7381 agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc
     7441 gcttcctgcg gcagctgcgc aactgcttca aagtagtagc aaatctgcgc cagcaaccgg
     7501 ctaatgttaa tcgagttagc tgagtttaac cccagcgcca ctttcagctc ttcatcatca
     7561 aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg
     7621 atattgccgc ccaatgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga
     7681 taaaggataa ccactttcac attcggtaaa ccgtagaaag catgagccac tgccgctccg
     7741 gtatcaccgg aggtcgcggt cagaatggtc actggcttat cgcccgcaat atgggtcagc
     7801 atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat
     7861 tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg
     7921 cgcacgcgct cttccaggat ttcctgcggg atttcatcac caataaacgc cgagaggatc
     7981 ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt caggctgaat
     8041 tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct
     8101 tgcgcaaagc tgacctgctc attgtgatct ttaagattgt agagtttcat ttagttttcc
     8161 agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc
     8221 aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacacaga
     8281 gcaaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc
     8341 gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc tgcgataaca
     8401 tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcct
     8461 gccaagtgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc
     8521 cgggcttctg ccgtcgagac tttaatcccc ggatacgcca gcacccacag ccactcatca
     8581 aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca
     8641 agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agattcgtcc ttccagctcg
     8701 cccatcaaag ccagcaaacg ggtgtcatta agcggcttgc cgcagtgttc attcatcgcc
     8761 atcagagccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat cggcatattc
     8821 ttttccagag tcatcgccac tggaatttgc ttgcccagct cctggcaaaa acgctcccag
     8881 cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagg
     8941 ttgttgagac tgaatgtctc tgccgactca accgtgacta catctccgag caatgcacca
     9001 tcaacgggtg tcaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa
     9061 gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag
     9121 atcggcaaag acaccggcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa
     9181 cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt
     9241 gaacagcgga tcattaccat ccacttcggc aatcttcacg cggcagacgc catcttcatc
     9301 aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc
     9361 aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcagcgtt
     9421 aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt cacgtcccgt
     9481 ttcacgagcg agaattaata gtttacgcgc tacatccata ccagaaagat catctcgcgg
     9541 atccggttcg gtataaccca tttcccgcgc cagcgtagtc gcctcggaga aactcatgcc
     9601 ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcat
     9661 caattcatca ccagcattga gcagattttg caggttctca ataaccggta atccagcccc
     9721 aacgttggtg tcatagagga atttacgccg cgatttttca gccgcatgac gcaacagatg
     9781 gtagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc
     9841 gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac
     9901 cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc
     9961 tttggcttgc gccagttctt cctgccagtt ttccagattt aggccatgca cattggtgag
    10021 cagagccttc gagttagcaa caccgcagac acgtaagtcg atatgtttat tcttcaacca
    10081 gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac
    10141 aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt
    10201 ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat
    10261 agcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg
    10321 caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg tcaccgccag
    10381 cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc
    10441 tcgcacacag tcgctttgcg gaacgcagaa actgatactg tattcggaag atgattgcgt
    10501 aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg ccgccatgcc
    10561 gaccattcct ttcatccccg ggccggaaac gctgaacatt gccatattat tcagattgga
    10621 aatgcccttg accggtaatt cgtcttcatc acgactggca ccaatgagcg tacctggagc
    10681 ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat
    10741 ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga
    10801 catcgatttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat ataccccgtc
    10861 aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga
    10921 gtagtcggaa ccgttgcgtc caagtaccac cagttcgcct ttttcattac cggcggtgaa
    10981 acctgccatc agcaccatgt gatcagccgg aatacgactt gccgcaatac ggcgggtgga
    11041 ctctgcaata tcgacagtag attcgaggta atgccccact gccagcagtt tttcgaccgg
    11101 atcgataacg gtaacgttgt gaccgcgcgc ttctaatacg ccggccataa tggcgatcga
    11161 cattttctcg ccacggcaaa tcagcgcagc gttaatgcta tccgggcact gccctaacaa
    11221 actaatgcca tgcaggacat gttttatttg ggcaaattcc tggtcgacga aagttttcaa
    11281 ttgcgccagc gggaatcccg gctgggcggc ggcgagtccc gtcagaagtt cggcaaaaat
    11341 acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat
    11401 cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg
    11461 cctggcattg ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat ttgccactga
    11521 tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa
    11581 agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg
    11641 ttacctgtgg taatggtaat ggtgatggtg gtggtgatgg tggtggtaat ggtggtgcta
    11701 atgcgtttca tggatgttgt gtactctgta atttttatct gtctgtgcgc tatgcctata
    11761 ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac ggcaggtaac
    11821 cagttcagaa gctgctgtca gagactcttt ttttaatcca cacagagaca tattgcccgt
    11881 tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt ggtgatattt
    11941 ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc taaaagcccc
    12001 aggcgttgtt gtaaccagtc gaccagtttt atgtcatctg ccactgccag agtcgtcagc
    12061 aatgccatgg ctcgttcgcg taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt
    12121 ttcgtcggtt gttgtattaa tattgctaat tgatagcaat agaccatcac cgcctgcccc
    12181 agattgagcg aaggataatc cgccaccatc ggcacaccag taagaacgtc agccaacgct
    12241 aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc
    12301 catgaagatt tttcctctaa cagcggcacc agttctactg gcgtagcgta gtaatgatat
    12361 ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc
    12421 agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc gacccagcgg
    12481 gtggctggct ccaggtgtgc ctgactatcg acaatccgca gttcgctaaa ccccatcgtt
    12541 ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac cagaataatc
    12601 gttatacgca tattgccact cttcttgacc aaatgaccgc gaacagggtg atcactgtca
    12661 acttattacg cggtgcgaat ttacaaattc ttaacgtaag tcgcagaaaa agccctttac
    12721 ttagcttaaa aaaggctaaa ctatttcctg actgtactaa cggtttaatt gttaaaaaat
    12781 gct
//
LOCUS       DAZGXS010000119        12468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGXS010000119 DAZGXS010000000
VERSION     DAZGXS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            159..2300
                     /locus_tag="ACHKG5_003700"
     CDS             159..2300
                     /locus_tag="ACHKG5_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP0869714.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKG5_003701"
     CDS             complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKG5_003701"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0869715.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKG5_003702"
     CDS             complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKG5_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0869716.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKG5_003703"
     CDS             complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKG5_003703"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0869717.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5718..6140)
                     /locus_tag="ACHKG5_003704"
     CDS             complement(5718..6140)
                     /locus_tag="ACHKG5_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0869718.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKG5_003705"
     CDS             complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKG5_003705"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0869719.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKG5_003706"
     CDS             6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKG5_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0869720.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8604..9275
                     /locus_tag="ACHKG5_003707"
     CDS             8604..9275
                     /locus_tag="ACHKG5_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0869721.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9281..10441
                     /locus_tag="ACHKG5_003708"
     CDS             9281..10441
                     /locus_tag="ACHKG5_003708"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0869722.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKG5_003709"
     CDS             complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKG5_003709"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0869723.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKG5_003710"
     CDS             11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKG5_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0869724.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKG5_003711"
     CDS             complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKG5_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0869725.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         3122 a         3314 c         3068 g         2964 t
ORIGIN      
        1 attttttatt atttctcatt tactactaca ttaccgcatg attgaatgat tctcaattac
       61 atatccgatg gtaaaataac aaacggatag tgcttagtga gcaacaagaa acaattttca
      121 ctcacgacaa tccgtgagtt tttgggataa cagactaaat ggctaagttc acaccttcat
      181 tctcaggaat caaaggtcgg gcgctctttt cactgctctt tgcagcacca atgattcatg
      241 caaccgacac tgcaacgacc aaagatggcg aaacaatcac tgttacagcg gatgcaaata
      301 ccgcaactga ggcgaccgat ggttatcaac ctctgagcac ttccacggcg acattaaccg
      361 atatgccgat gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa
      421 atcagaatgc aacgacgctg gatgaagcgc tttataacgt cagtaacgtg gtacagacca
      481 atacattagg cggaactcag gacgcctttg tacgtcgtgg ttttggtgct aaccgggatg
      541 gctccatcat gaccaacggc ctgcgaactg tacttcctcg cagtttcaac gccgccacag
      601 aacgtgtgga agtgctaaaa ggtccggcct ccacgctgta tggcattctc gatcctggtg
      661 gattgattaa cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttct gtctcagcca
      721 cgtcctccag ttttggcgga ggcactgggc aacttgatat cacaggtccc attgaaggca
      781 ctcagctggc ataccgcctg acgggggaag tgcaggatga agattactgg cgaaacttcg
      841 gtaaagagcg cagtacattt attgccccgt cactcacctg gtttggtgat aatgcaacag
      901 taaccatgct ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg
      961 accttacgac gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta
     1021 atattacaga tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc
     1081 agtggacagc gcgctttgat tacagctaca gccaggataa atacagcgac aatcaggctc
     1141 gcgttaccgc gtatgatgca acgacaggaa cgctgacacg gcgtgttgat gcaactcagg
     1201 gatctaccca gcgtatgcat tctactcgtg cggatctgca agggaatgtt gatattgctg
     1261 ggttctataa tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgcacag
     1321 atatgattcg ctgtaaaaac gctaaagatt tcaatatcta caaccccgtt tatggcaata
     1381 ccagcaaatg cacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct
     1441 actcagctta tgcacaggat gcgctctatc tgaccgataa ctggattgcc gtcgccggga
     1501 tccgctatca gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg
     1561 acagccgcga tgaacaatgg acgcccaaac tggggttagt ctacaaactg acgccatcgg
     1621 tatccttatt tgctaattat tcgcaaacat ttatgccgca atcgtcaatt gccagctaca
     1681 tcggagatct tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt
     1741 tcgatggtat aaccgcagat attgcgctgt ttgatatcca taaacgtaac gtgttgtata
     1801 ccgaaagtat tggtgatgaa accatcgcca aaacggcagg ccgcgttcgt tcaagagggg
     1861 tagaagtcga ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct
     1921 ataccgatgc taaggttctg gaagatcctg attatgcagg gaaaccattg ccgaatgttc
     1981 ctcgtcatac cggttcgcta ttcctgacct atgacattca taacatgcca ggcaataaca
     2041 cactgacgtt tggcggtggt ggacatggcg taagccgtcg ttcggcaacc aatggggctg
     2101 actattatct gccaggctat ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc
     2161 agtatccggt cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct
     2221 cttccatcgc cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca
     2281 cggtgaaaat ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt
     2341 ttattcttct ggcgggagga ttatcagacc tcaatctccg ccatgtcgcc tttctcttgc
     2401 aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca
     2461 gtacgctgat cgtcttcatc atcgatagtc agctgcacca gacggcgagt gttcggatca
     2521 agcgtggttt cgcgcaattg catcgggttc atttccccca gacctttaaa acgctggacg
     2581 ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc
     2641 agcgcgtaat aaacctcttt cccgagatca atacggtaga gcggtggcag tgcgacgtaa
     2701 acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga acaaagcgca gagcagtgta
     2761 gcaatgtgca aaccatcaga gtccgcatcc gcgaggatac agattttgcc ataacgaagc
     2821 tggctcagat cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc atgcacttcc
     2881 tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt taaggatctt acctttcagc
     2941 ggcatgatcg cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca
     3001 ccttccacga ggaacagttc ggtacggtta aggtcctgcg cggtacaatc agccagtttg
     3061 ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca cttttttagc cgcacgcata
     3121 cgacgctggg cgctggaaat cgccatctct gccagcaact ccgccgcctg aacgttctgg
     3181 ttcagccaca ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc acactgacgc
     3241 gaagagagac gctctttggt ctgcccggca aactgtggat cctgcatttt tactgacagc
     3301 acataggcgc agcgatccca gatatcttcc gccgacaact ttacaccgcg cggcaaaatg
     3361 ttgcggtatt cacagaactc acgcatcgcg tccagcaggc cctgacgcag gccattaaca
     3421 tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg
     3481 ccttccggca gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg
     3541 ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc
     3601 agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc
     3661 tcaacgccgg ggcacagtac tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa
     3721 aaacgcgggc tatcgaagaa ggtttcatcc ggccagaaat ggacgctggt gccggtattg
     3781 cgtttaccgc aattgccgac aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg
     3841 atgttataaa cctgaccatc gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta
     3901 accaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt gttagagaat
     3961 ttaccgcctg catgcagacg gcaaagaatc agttcaaccg ccggtacacc ctcttccggg
     4021 tgaatatcca ccggcatccc gcgcccatcg tcaataactt ctaacgactg gtcagcatgt
     4081 aaaataacgt ccacgcgttt tgcgtgaccc gccagtgctt catccacact gttatcaatg
     4141 acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga
     4201 accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt
     4261 taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg
     4321 gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg
     4381 gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg
     4441 cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt taagatcgta
     4501 aatatggcgt gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag
     4561 atagtccgtc agcagttcaa acgggcgcac cgccgggttt accaccactg cgggcagcat
     4621 aaaacattgt gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag
     4681 cgaatcgccg ccatgttcca ggacaatgga ttccagcagc tctgccgcgt cggaaggata
     4741 cggcggcaac tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt
     4801 taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt
     4861 agacatcagt agccttctga agcggtatca ggttggaaac gtgtgtccgc caggcgatgc
     4921 acctcggtgg tcagcgtgcc atcagcatgt aactcgagag tacgccagcc gggcgcgata
     4981 gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga cggtgttgcc
     5041 agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag
     5101 tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg
     5161 tgttgatcaa gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta
     5221 tggcgctctg gcgcatcagc cagtttacgt tccagccact caagctgaaa ctcgcttagc
     5281 tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca
     5341 ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcgctgta catcgcgggc
     5401 tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc gatgccttca
     5461 gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc
     5521 aggtcgaatt cgtgctggtg tggacgaatc gcctccagca ccgcctggta gctttcccag
     5581 gtgtttaccc ctaagagggc ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt
     5641 aaaatcctga ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt
     5701 ccttaggttt cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat
     5761 cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt
     5821 ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca
     5881 gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc
     5941 gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc
     6001 agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc
     6061 ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag
     6121 tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc
     6181 agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg
     6241 tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg
     6301 tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg
     6361 gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagcccc
     6421 gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatt
     6481 ccggcaacca tctccagtag ccaaggggtt tcgctagtgt cgtatgcggc aattcgaatc
     6541 tgctcaatca gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga
     6601 ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga
     6661 cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt
     6721 tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc
     6781 ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt
     6841 catgtgttaa attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta
     6901 gcggcttctg ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac
     6961 cacaaggaat gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctttctgggt
     7021 tcagttcgtt gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta
     7081 acccggaatt gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag
     7141 cgcgcagtcc attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct
     7201 accgcgacgc gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat
     7261 ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gcagggattc
     7321 aggacgtcac gtatcagacc gatcagcaaa ccttgatcct caacaccgcg accgcttatt
     7381 tcaacgtgtt gaatgctatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct
     7441 accgtcaatt agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcaccgacg
     7501 tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata
     7561 accttgataa cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg
     7621 cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac gcgctgctga
     7681 aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag gacctggcgc
     7741 gcgagcaaat tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta
     7801 gcgggatttc tgacacctct tatagcggtt cgaaaacccg tggtgccgct ggtacccagt
     7861 atgacgatag caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc
     7921 agggcggaat ggttaactcg caggtgaaac aggcacagta caactttgtt ggtgccagcg
     7981 agcaactgga aagcgcgcat cgtagcgtcg tgcagaccgt acgttcctct ttcaacaaca
     8041 ttaatgcttc tatcagtagt attaacgcct acaaacaagc cgtagtttcc gctcaaagct
     8101 cattagacgc gatggaagcg ggctactcgg tcggtacgcg taccattgtt gatgtgttgg
     8161 atgcgaccac cacgctgtac aacgccaagc aagagctggc gaatgcgcgt tataactacc
     8221 tgattaatca gctgaatatt aagtcagccc tgggtacgtt gaacgagcag gatctgctgg
     8281 cactgaacaa tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa
     8341 cgccggaaca gaatgctatt gctgatggtt atgcgcctga tagcccggca cccgtcgttc
     8401 agcaaacatc cgcacgcact accaccagta acggtcataa ccctttccgt aactgatgac
     8461 gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc
     8521 gcattcttcc cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt
     8581 accacaggtc ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc
     8641 gcaaaaactg gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta
     8701 tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact
     8761 gttcagctgc aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga
     8821 aagaagccga acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg
     8881 gtgaaggtca gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc
     8941 aggcccagca atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggtc
     9001 gtctgatggg cggcggtgcg ggatttgcac agcagccgct gttctcctcg aaaaacccag
     9061 ccagcccggc ttacggtaaa tataccgacg cgacgggtaa aaactatggc gcagcccagc
     9121 caggccgcac catgaccgta ccgaagacag ccatggcacc aaaaccggcg accaccacta
     9181 ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg
     9241 caaccggtac ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac
     9301 cgagcgcccg gactggcgtg agaaagccca cgaatacggt ttcaattttc acaccatgta
     9361 cggcgagccg tactggtgtg aagatgctta ctacaagttg accctcgccc aggttgaaaa
     9421 gctggaagaa gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat
     9481 cgccagcgat gagctgatga ccaaattccg cattccaaaa cacacctgga gttttgtgcg
     9541 ccagtcatgg ctgacgcacc agccatcgct ttattcgcgt cttgatctgg cgtgggatgg
     9601 cactggcgaa cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tatacgaggc
     9661 ggcgttcttt cagtggatct ggctggaaga tcagcttaac gccggtaact tgccggaggg
     9721 cagcgaccag tttaacagtc tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga
     9781 acagtatggc ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg
     9841 taccattcag tatttgcagg actgcgcgac ggaagctgaa atcgctaccg agttcctcta
     9901 cattgacgat atcgggttag gtgaaaaagg tcagttcacg gatttacagg atcaggtaat
     9961 ttccaacctg ttcaagctgt atccgtggga atttatgttg cgtgagatgt tttccaccaa
    10021 gctggaggat gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa
    10081 ggcacttcta ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcccgctta
    10141 ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg
    10201 tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc
    10261 gtatggcgaa gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag
    10321 ctatatgctg attggtagct ggctggtgaa cgatcaaccc gccggaattg gcattcgtga
    10381 agaccgtgca ttgatcaccc aggatatgtc tcggttttat ccacatattt ttgttgaata
    10441 atcaatgatg ccggatggca ttcgccatcc ggtaattgtt aacctatctg caccgacagc
    10501 atacttaaac tgcccatttc tataccatcg accggaatgg taattggctc ctgcccatcc
    10561 cacgcaccta agacatacag caacggcaga tagtgctctg gcgttgggtt cgataacgcg
    10621 ccaccttcat ggtcgagata attcaccaga ggatgctgct ctaccggccc ttgccacgtc
    10681 agattcgctt tcacatactc attaaacgac atcgcccaag gatacggtga actatcaccg
    10741 tgccacttca ctgtacgcag gttatgcacc acgttgccgc tggcgaccaa cattattcct
    10801 tcatcacgca gcgccgcgag ttttcgcccc atttcgaaat gccaggcggc aggtttgcta
    10861 ctgtcgatac tcaactgcac catcgggata tccgcgtcag gatacatctt aatcagcacg
    10921 ccccaggaac cgtggtcaaa gccccaggct tctttatcca gcgtcaccgg gatcggcgct
    10981 aacagctcaa ccagacgctg tgccagcgca ggcgaacccg gagcaggata atgcgtatcg
    11041 tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc
    11101 actcctgttc cacgggtaaa ccagtgagcc gaaactacca caatcgcttg cgggcgtggc
    11161 aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat tatcttccag cacgttcatc
    11221 ggactaccgt gacctaaaaa caatgctggc atacgtgttg aagacatgat gatatcctta
    11281 actaaaggtg tcattttgat atcctcacga tacgcttgtt cggcggagta agaacccgga
    11341 taacaatgat gatgatcatc agttattttg acgatctgcc tgaaggtgaa gatttataag
    11401 gagttgtcga tgtcagtacc tctcattctg accatactgg cgggggcagc cacgtttatt
    11461 ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta
    11521 ggatttgcgg cggggatcat gttgctcatc tcattaatgg aaatgcttcc tgccgcacta
    11581 gcagctgaag gaatgtcgcc tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc
    11641 tattttggcc tggaccgcat gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg
    11701 gtgcagccgt tgccaaaatc gatcaagcgc acagccattc tgctcactct cggcatcagt
    11761 ctgcataact tcccggaagg gattgccacc tttgtcacgg cgagcagcaa cctggagctg
    11821 ggatttggca tcgcactggc cgtcgcgttg cacaatattc ctgaaggtct ggcggttgca
    11881 ggcccggttt atgcagcaac gggttctaaa cgcacggcaa ttctgtgggc ggggatttct
    11941 ggactggcag aaattcttgg tggtgtgctg gcgtggttaa tcctcggtag catgatttcc
    12001 ccggtggtca tggcagcaat catggcggcg gtcgcaggaa ttatggtggc gctctcggtt
    12061 gatgaattaa tgccgctcgc caaagagatt gaccctaata ataaccccag ctatggcgta
    12121 ctatgtggaa tgtcagtgat gggattcagt ttggtgctgc tacaaacggc gggaattggt
    12181 taaaagcaac atcagaagtt gtccagcgca gggacaactt ctgctagttt cactaaagcc
    12241 ttattctacc ttttcggctg tctcttctct cgtactgttt aagtatttgt tccgcgcttc
    12301 aagcagtgcg tctctgtcct ggcgaaatgc ccgacaataa cgtttcaaat gcaggaaata
    12361 acccaccaca accagaacaa tcaatacaat ccagtaccag gcgataaaca tccttttacc
    12421 cttaatattt aacaatacta aacatttagc gcataaattt cacatatc
//
LOCUS       DAZGXS010000120        12207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGXS010000120 DAZGXS010000000
VERSION     DAZGXS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            154..582
                     /gene="uspC"
                     /locus_tag="ACHKG5_003712"
     CDS             154..582
                     /gene="uspC"
                     /locus_tag="ACHKG5_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0869726.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(589..2013)
                     /gene="otsA"
                     /locus_tag="ACHKG5_003713"
     CDS             complement(589..2013)
                     /gene="otsA"
                     /locus_tag="ACHKG5_003713"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0869727.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1988..2788)
                     /gene="otsB"
                     /locus_tag="ACHKG5_003714"
     CDS             complement(1988..2788)
                     /gene="otsB"
                     /locus_tag="ACHKG5_003714"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0869728.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2955..3941)
                     /gene="araH"
                     /locus_tag="ACHKG5_003715"
     CDS             complement(2955..3941)
                     /gene="araH"
                     /locus_tag="ACHKG5_003715"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0869729.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKALGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3956..5470)
                     /gene="araG"
                     /locus_tag="ACHKG5_003716"
     CDS             complement(3956..5470)
                     /gene="araG"
                     /locus_tag="ACHKG5_003716"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0869730.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5540..6529)
                     /gene="araF"
                     /locus_tag="ACHKG5_003717"
     CDS             complement(5540..6529)
                     /gene="araF"
                     /locus_tag="ACHKG5_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0869731.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7326..7829
                     /gene="ftnB"
                     /locus_tag="ACHKG5_003718"
     CDS             7326..7829
                     /gene="ftnB"
                     /locus_tag="ACHKG5_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0869732.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7909..8160)
                     /gene="yecJ"
                     /locus_tag="ACHKG5_003719"
     CDS             complement(7909..8160)
                     /gene="yecJ"
                     /locus_tag="ACHKG5_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP0869733.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8275..8361)
                     /gene="azuC"
                     /locus_tag="ACHKG5_003720"
     CDS             complement(8275..8361)
                     /gene="azuC"
                     /locus_tag="ACHKG5_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0869734.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8624..8947
                     /gene="yecR"
                     /locus_tag="ACHKG5_003721"
     CDS             8624..8947
                     /gene="yecR"
                     /locus_tag="ACHKG5_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0869735.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNKEFTLSYQCHHSA
                     FPVFL"
     gene            9118..9615
                     /gene="ftnA"
                     /locus_tag="ACHKG5_003722"
     CDS             9118..9615
                     /gene="ftnA"
                     /locus_tag="ACHKG5_003722"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0869736.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9652..9891)
                     /gene="yecH"
                     /locus_tag="ACHKG5_003723"
     CDS             complement(9652..9891)
                     /gene="yecH"
                     /locus_tag="ACHKG5_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0869737.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10083..11294
                     /gene="tyrP"
                     /locus_tag="ACHKG5_003724"
     CDS             10083..11294
                     /gene="tyrP"
                     /locus_tag="ACHKG5_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0869738.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11356..12021)
                     /gene="yecA"
                     /locus_tag="ACHKG5_003725"
     CDS             complement(11356..12021)
                     /gene="yecA"
                     /locus_tag="ACHKG5_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0869739.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT         3089 a         3062 c         2810 g         3246 t
ORIGIN      
        1 tgaaatcata tgatcaccga gagtgattga ttaattgtct ttactaataa tcagactaat
       61 atttacctgt ttgactgagt tgggattgcg tcgtttctcc attaggagta aagctttaat
      121 gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat tcttgtcgct
      181 gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat cgccaggcca
      241 gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta caatcaatta
      301 gctgcgccga tgcttgaaga tttacgcagc gtgatgcagg aagaaacgca aagctttctt
      361 gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag
      421 ttaagcgaac atattctgga agtttgtcgc aaatatcatt tcgatttggt gatttgcggt
      481 aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca
      541 agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct acgcaagctt
      601 aggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt
      661 taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag
      721 catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc
      781 agctgcaact tcgtcacgat cgtagggatt aacaattaac gccgacgtta actcgtttgc
      841 cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcggcaac
      901 atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga
      961 gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg
     1021 cgtccagcct aattgcccgt atttaccatt aattcgtccg gcttcatttt cgagctgatg
     1081 acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct gggtataacg
     1141 aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga gaaaacgctc
     1201 tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta cgtttttcag
     1261 ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt tggctatttc
     1321 ttttggctca atgccaatcg ggtaaacttc tgttcgaaac gctttgcccc aggctgtatg
     1381 gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa aggcaatcca ggaacgccag
     1441 acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt caagcaaggt
     1501 gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat gcagaaagaa
     1561 accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaatggca acaggtgata
     1621 atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa
     1681 cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgaggcg
     1741 ataatgaaaa gcaggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc
     1801 ctgttcgctg aggttaaaag aggcccacgt aatgttaccc tttttcacct tttttagcgg
     1861 ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag
     1921 tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcat ctggtggtgc
     1981 aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt
     2041 tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggt acgcccgcca
     2101 gtcgccatga tgcctgagtt gcgcctgtgc caatttttac tgacattccg cccagtcggt
     2161 taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc
     2221 cgataaaggg agcttcctgc ataaaggcag caatcgcctc acctttactg gtacctctcg
     2281 gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa
     2341 tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg
     2401 caaaagccat ccctttcgtc tccagctccg caccgggata ctgggcgatg accgtatgca
     2461 gttgcacgtt aatatcacgt gcaatcgcat ccggcagatg aacgatatgc gttttaccat
     2521 tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca
     2581 gtgcgtccag ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg
     2641 ttgccagtag ctgaagtcct tgcagaatat tgtcaggtac gacgacctga tcgggatgcg
     2701 gtttgatttc cgccagcgtt ccatcgagat caaaaaacca ggcatatttc gcggatagtt
     2761 caggggtttc ggttaacggt tctgtcaccc ggttctcctc cttctctttt cttattgtgt
     2821 tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg tcgccatttg
     2881 caacgaagaa cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac gttaaattgt
     2941 tgtggaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacggtc gaagatcacc
     3001 gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg
     3061 ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt
     3121 ccgatgccac ctttcagaga aacgccacct aaaacgcagg cggagataac aataagctca
     3181 taaccaatcg acgtcattgg ctgcccactg gtcatacgtg aagccagaat aattccggct
     3241 atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgagc aaccggtaca
     3301 cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtattacg accaaaggta
     3361 gttttattca gcagcaaacc aaagataatc agacacgcga cggtgagcca gattggcgca
     3421 ggcagaccga accagttggc gtaaccaagg gcaaagaagc tttcatcttc gataccgagc
     3481 gctttaccgt cggaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtc
     3541 gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga
     3601 atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag attgataacc
     3661 accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg
     3721 gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc
     3781 aaccctttca tattaataaa ggtggcaaag ttcgggacaa aaatggcaca ggcgataaaa
     3841 agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac
     3901 gacttaggtg cgccagaacc cgatgtagaa acagaagaca tcatactctc cttactcagg
     3961 caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct
     4021 cgtgtaacaa ttcaccggcg atttcacctt cgcgcatcac cacaatccgg tcggcaacgc
     4081 cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc gccgccagcg
     4141 cataaatcac gttatatatt tcgtgcttag caccaacatc aatgccgcgc gtaggttcat
     4201 ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat
     4261 ttccacctga gagattcata atcagttgct cagcgcccgg cgttttgatg ttgagcgaac
     4321 gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa ccgccgagca
     4381 catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcaca ggaataatgc
     4441 cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt
     4501 tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc
     4561 caaacatgcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac agcccaacaa
     4621 tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct ttcacagcat
     4681 caagacgcag gcgctcctcg ccataactac gcggttgcca gccgtagata tcgccaatgt
     4741 cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg
     4801 ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt
     4861 ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc
     4921 ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa
     4981 ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga ccaatggaga
     5041 gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg
     5101 cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat
     5161 agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca
     5221 cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta
     5281 cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg
     5341 cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg
     5401 tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag
     5461 actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaaa
     5521 ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa
     5581 agttgtcacg cgtgatcagc accacgtcgg taacttcggt gaatttggtc ggttcaacac
     5641 cttttgctac ccagttgtaa agcatttcgc tggacttata accatgtacg tccgggcttg
     5701 gcaacaggga accgtagaag ccggttgcct gtgccttaga cagttcgctc accgcatcca
     5761 caccgttaat accgataccg atgatatcgg ccgctttaaa gccctgacct tccgtcgcgc
     5821 gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg
     5881 gatgttgaac cagcattgag ttggcagcgt caaatgcccc ggggatgtcg ttagatttgg
     5941 taggtacctg ataaatttgt ttttccggga atcctgcagc tttcagcgca tccatagaac
     6001 ccgtagtacg gcgacgagcc gtatccagtt cgttggaggt aatcgccatc accgcgcttt
     6061 ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctggccc tgacgttcgc
     6121 caattttggt cgccgccatc atcaccagag gaacggtatc cattggctta cctttggcgt
     6181 taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct ttcgccacga
     6241 tggcagagcc gagtttcggg tccggagtac aaataacgaa accttttgcg ccactggcgg
     6301 ccaggctatc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct
     6361 caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct
     6421 cttccggttg cttcaccagg aaaccgagct tcaggttctc cgccatagcg gattgtgaca
     6481 taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca
     6541 gctttagtgt cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt aatcggaaaa
     6601 caaagcatta ccttttaact aaaagataag tgactgtgtt gacatagttt tagcgagaaa
     6661 ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac gtgcataaat
     6721 ttagcgggaa aagacataag gaaaagccaa tttgtcagac aaattgttga atgcacagca
     6781 gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat gcgggatgtc
     6841 ttctttttca ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat
     6901 aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgtcattaa ttatcactgt
     6961 gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt
     7021 tataaaataa tcacttcatc atgaattcaa attcattgat taatatcaac aagatacaaa
     7081 aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa tgtaaaaata
     7141 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
     7201 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
     7261 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
     7321 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
     7381 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
     7441 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
     7501 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
     7561 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
     7621 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
     7681 tcctgcgcga tctggaaaaa gaacagcagc atgatggcct gttgctgcaa accattcttg
     7741 atgaagtgcg cagtgcgaaa cttgcgggta tgtgcccagt gcagaccgac caacatgttc
     7801 tgaatgtcgt gtcgcatcag ctgcattgat catacatcga cgctaatgca ttgcaccgat
     7861 gaagtttttg agaaaccgct gcctcatctt ttttgaagca gcggtttttt aatgggattc
     7921 gccttgtggg gtaaacttga gctcaatcag cgcgatggct ttttggattg cccgcatggt
     7981 gaccgggtct gcggcagcgg gatgatgagt aaagtcgata ttcttcagct gactggacat
     8041 tttttcacga acttcaacgg gcgcgattac atcgagaaca tccagaattt gtttgataac
     8101 caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg tctgggacat
     8161 cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcaacgat
     8221 agtcacatcc accctcatca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg
     8281 gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga acatactttt
     8341 caggattttg cgcagtttca tcgcggcact ccgaccattt gttatacagg tgttattgtc
     8401 tttgcgctta taatatgaca accatcacaa aaatcaatct ttatgtgata caaatcacat
     8461 aaatacccct ttaatgttat aaaaatgata atcaaagaac agccccccta tttctgacac
     8521 ctacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg
     8581 taacgcgtaa aatagtgctt tctcttactc ttctggctgg accatgagac ttctgattct
     8641 gactctttca ttaataacgc tggcggggtg tacggttact cgccaggccc atgtgagcga
     8701 agttgacgcg gcaacaggta ttgtacggtt ggtttatgac caggcttttt tgcagcatgc
     8761 ccataccgat cgttatgtca gtcgcgggat ctccgatcgc gcatgccaac aggaaggcta
     8821 tacccacgcg atcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt
     8881 atgtttgaat aaggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt
     8941 tctctaaata ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg
     9001 ttttattccc attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata
     9061 aaataacaaa ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg
     9121 ctgaaaccag aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca
     9181 ctgctttatc agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg
     9241 ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg
     9301 actgataccg gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc
     9361 tcacttgatg aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt
     9421 aacgaactgg ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag
     9481 tggtatgttt ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta
     9541 agcctggcag gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc
     9601 gacacacaaa actaatgctc tccggcggca ggtttctgcc gccatttcaa atcagtgacg
     9661 gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa ggtataaatt tgccttttgc
     9721 tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcaag tgtgaaaacg
     9781 tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc
     9841 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc
     9901 tcaaaaaaaa gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact
     9961 gaatgctttt tattgtacat ttatgtttac accatatgta acgtcggttt gacgaagcag
    10021 ccgttatgcc ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa
    10081 gcgtgaaaaa cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag
    10141 gcatgctggc aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt
    10201 tgattgggct ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc
    10261 atgttccggc agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc
    10321 aatggctgac gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca
    10381 gcggtgccgg tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg
    10441 caaccgctgg cgtgttgttg ttcacttttg ttgccggtgg cgtggtttgt gtcgggacat
    10501 cgctggtcga tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga
    10561 tgctggtatt gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac
    10621 aggggctggc actgtctgca atcccggtga tttttacgtc gtttggtttt cacggtagcg
    10681 tgccgagtat tgtcagctat atggatggca acattcgtaa gctacgctgg gtgtttataa
    10741 ccggtagtgc gatccccctg gtggcatata ttttctggca ggtggcgacg cttggcagca
    10801 ttgattcaac aacctttatg ggattgctgg ctaatcatgc cggattaaac gggctgttac
    10861 aggcgttacg cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg
    10921 atttagccct tgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggcag
    10981 atttgtttca gcgttcaaat accgttggtg gacgattgca aactggagca attacctttc
    11041 tgccaccgtt ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg
    11101 ccggtgtggc gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca
    11161 gaaagcacaa tcctcaagcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg
    11221 tgtttctctg tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac
    11281 cagaagtggg gtgattagat agcctcaaat tccttattgg gtgccagaat taacgctgac
    11341 acccaatttg acttcttaat gcaggcagca ctgcttaaat tttttaccac taccgcacgg
    11401 gcaaggatcg ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc
    11461 ctgcggatgc gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac
    11521 gctctcttca aacgcttctg gcgacatctt ctctacccgc tcgaagtttt cctcagtacc
    11581 gtgcagcgct atcgcctcca gcgctggttt taacgaatcg ggcaatgttg accagtcaga
    11641 aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc
    11701 atcaacttct cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc
    11761 tgcggtatcg gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga
    11821 cgcccagcgc ggcacatagt cagccccacc ccacacggca accagccact gtgccggttc
    11881 aatctcttgc ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag
    11941 gatggcgtgg tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc
    12001 gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc
    12061 ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatca
    12121 aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca
    12181 ctgcttattg ctttgatttt attctta
//
LOCUS       DAZGXS010000121        12163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGXS010000121 DAZGXS010000000
VERSION     DAZGXS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            227..997
                     /gene="yjjP"
                     /locus_tag="ACHKG5_003726"
     CDS             227..997
                     /gene="yjjP"
                     /locus_tag="ACHKG5_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0869740.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            988..1461
                     /gene="yjjB"
                     /locus_tag="ACHKG5_003727"
     CDS             988..1461
                     /gene="yjjB"
                     /locus_tag="ACHKG5_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0869741.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1568..2107
                     /gene="dnaT"
                     /locus_tag="ACHKG5_003728"
     CDS             1568..2107
                     /gene="dnaT"
                     /locus_tag="ACHKG5_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0869742.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQAVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2110..2847
                     /gene="dnaC"
                     /locus_tag="ACHKG5_003729"
     CDS             2110..2847
                     /gene="dnaC"
                     /locus_tag="ACHKG5_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0869743.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2896..3390
                     /gene="yjjA"
                     /locus_tag="ACHKG5_003730"
     CDS             2896..3390
                     /gene="yjjA"
                     /locus_tag="ACHKG5_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0869744.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3644..5935
                     /gene="opgB"
                     /locus_tag="ACHKG5_003731"
     CDS             3644..5935
                     /gene="opgB"
                     /locus_tag="ACHKG5_003731"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0869745.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6072..7094)
                     /gene="lgoD"
                     /locus_tag="ACHKG5_003732"
     CDS             complement(6072..7094)
                     /gene="lgoD"
                     /locus_tag="ACHKG5_003732"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0869746.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7233..8147
                     /gene="lgoR"
                     /locus_tag="ACHKG5_003733"
     CDS             7233..8147
                     /gene="lgoR"
                     /locus_tag="ACHKG5_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0869747.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8362..9723
                     /gene="lgoT"
                     /locus_tag="ACHKG5_003734"
     CDS             8362..9723
                     /gene="lgoT"
                     /locus_tag="ACHKG5_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0869748.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9772..11436)
                     /gene="tsr"
                     /locus_tag="ACHKG5_003735"
     CDS             complement(9772..11436)
                     /gene="tsr"
                     /locus_tag="ACHKG5_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0869749.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11665..>12163
                     /locus_tag="ACHKG5_003736"
     CDS             11665..>12163
                     /locus_tag="ACHKG5_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869750.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVL"
BASE COUNT         3006 a         3168 c         2922 g         3067 t
ORIGIN      
        1 gacccgtaaa ggctaaaaca ataaattcat tatagtgata atattcatgt gatagtttgt
       61 ttgcggcgag agataattcg ctttttatca ccgagcttgt cccgataacc aggattaatc
      121 agcgcaattt tgatcttaac ctaccgcaaa acaaccttgc ttcatgatac aaatggattc
      181 tcattatctg aacaatacgc agcacgtcta tgacaaaggg cgagttatgc aaactgagca
      241 acagcgagcc gtaacacggc tttgtatcca gtgtggatta tttcttttgc aacatggtgc
      301 ggaaagcgcg ttggttgatg agctttcctc acgactgggt cgggcgctgg gaatggacag
      361 cgtcgaaagt tctatctctt cgaacgccat agtgctgaca actattaaag atgggcaatg
      421 cctgacatcg acacgtaaaa atcacgatcg cggcattaat atgcatgtgg tgactgaagt
      481 ccagcacatt gtgattcttg cggagcatca tctgctggat tacaaaggcg tagagaaacg
      541 atttagccaa attcagccat tacgttaccc aagatggctg gtagccttaa tggttggcct
      601 ttcttgcgcc tgtttctgta aactcaataa cggtggctgg gatggtgccg tcatcacctt
      661 ctttgccagt acgaccgcga tgtatatccg ccagctgctg gcacaacgtc atcttcatcc
      721 acagatcaac ttttgcctta ccgctttcgc cgccaccacc atttccggat tgcttttgca
      781 acaccccact ttcagcaata cccccaccat tgcgatggcc gccagcgttc tgctgctagt
      841 gccgggcttt ccgttgatta atgccgtcgc cgatatgttt aaaggccaca tcaataccgg
      901 actggcacgc tgggcgatcg ccagtctgct gacactggct acctgcgtcg gcgtagtgat
      961 ggcactgacg atttgggggc tacgcggatg ggtgtgatcg aatttctgtt agcgttggcg
     1021 caggatatga tcctcgccgc cattcctgcg gtcggctttg cgatggtgtt caacgttccc
     1081 gtgcgggcgt tacgctggtg tgcgctgctt ggctcgatag gtcatggttc ccgaatgatc
     1141 ttgatgacca gcgggttgaa tattgagtgg tcaaccttta tggcctccat gctggtcggc
     1201 accattggta ttcaatggtc gcgctggtat ctggcgcatc cgaaagtgtt taccgtggcg
     1261 gccgttatcc ctatgttccc gggcatatcg gcttataccg caatgatttc ggcggtaaaa
     1321 atcagccagt taggttacag cgaaccgttg atgattaccc tgttaaccaa ctttcttaca
     1381 gcttcatcga ttgttggtgc gttatccatc ggtctttcca ttcctggatt atggttgtac
     1441 cgcaagcgcc ctcgcgtata aaattgcctc gcttaagcac acggatgaga gacagcctcc
     1501 tctcctccgt gtgttactat aaaagttatc tcccttctcg ttcatcgttc catatttgag
     1561 aaacagtatg tcttccagag ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca
     1621 cgatcaccaa gccgttctgg caaaagctga aggcggtgtg gttgccgtat ttgctaacaa
     1681 tgccccggcg ttttatgccg tcacgcctgc acgcctggct gaactgctgg cgctggaaga
     1741 aaagctggcg cgtccgggaa gcgatgtcga tctggacgat caactctatc aggaaccgca
     1801 agccgctccc gttgctgtac ccatggggaa attcgccatg tatccggact ggcaacccga
     1861 tgccgacttt atccgcctgg cggctctatg gggcgtggcg ctaagagagc cggtaaccac
     1921 cgaagaactg gcctcattca ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt
     1981 gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca acggcggact
     2041 gccgaaacga gatgtgaata cggtcagtga acctgacagc caaattccac caggattcag
     2101 agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc
     2161 catatcaaac ctgcattcaa aacgggtgaa gagctactgg cctggcagaa agaacaaggg
     2221 gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt
     2281 aaccgctccg gtattcgtcc actgcatcag aactgctcct ttgagaacta tcgcgttgag
     2341 tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt atgtcgaaga gtttgacggc
     2401 aacatcgcca gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg
     2461 gcaatctgca acgagctgct gctacgcggt aaatccgtat tgatcatcac cgtggccgat
     2521 attatgtcgg cgatgaaaga taccttcagg aatagcggta ccagcgaaga acaactgctt
     2581 aacgatctga gcaacgttga tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg
     2641 aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc gctcttcttc caaacgccca
     2701 accgggatgc tgaccaacag taatatggaa gagatgacca agttgctggg cgaacgcgtg
     2761 atggaccgta tgcgcctggg taacagtttg tgggtgatct tcaactggga tagctaccgt
     2821 agccgtgtaa caggtaaaga gtattaatcc ccgtttctgg cggtatactg cgacacaatt
     2881 ccaccttgag taatgatgat gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc
     2941 agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc
     3001 gccagcgaac ttggcaacca aaacagcacg acacaggaag gcggttggtc gctcgcgtca
     3061 ttaactaact tgcttagcag cggaaaccag gccttaagcg cagataacat gaacaatgcc
     3121 gcaggcattc tgcaatactg cgcgaagcaa aagctggctt cggtaaccga tgtcgaaaac
     3181 atcaagaacc aggtgctgga aaagctgggc ctgaacagtg aagagcaaaa agaagacacc
     3241 aactatctgg acggcattca gggtttgctg aaaaccaaag atggtcagca actcaatctg
     3301 gataacatcg gaacaactcc gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg
     3361 ttaaagcagg ggctgaactt catttcctga accgcgaaac gccgcgtttt agctgttacc
     3421 cggctgacgc ggctctctcc tgcccttttc cctgctcctg gacggttttc cccctgttac
     3481 acgggaagcg accgcgatca aaaaagtctg tttataaact aattcctaac aacatcgcat
     3541 ttttttgaca ctagaattgc agcaatatgg cgacgccatt acattgtgct tctcaaaaag
     3601 cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc ccgttgtcag aactactctc
     3661 tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg
     3721 gtggtttgca gccacattaa cggtgctggg gctatttgtc gttttaaata tcaccctgtt
     3781 tgccagcgac tattttactg gcgatggtat taacgacgcg gttctctata ccttaaccaa
     3841 cagcctgacc ggtgctggcg tcagcaaata cattttgccg ggtatcggca ttgtgctggg
     3901 gctgacagcg gtgttcggtg cgctgggctg gatcctgcgc cgtcgtcgcc atcatccgca
     3961 ccattttggt tacagcctgc tggcgctctt actggcgctg ggttcggtgg acgccagccc
     4021 ggcatttcgt cagataacgg aactggtgaa atcccagtca cgcgacggcg acccggactt
     4081 tgcggcttat tataaagagc cgtcgaaaac tatccctgac ccgaaactca acctggttta
     4141 tatctacggc gaaagtctcg agcggaccta ttttgataac gaggctttcc cggacctcac
     4201 gcctgaactg ggcgcgttga aaaatgaagg cctggatttc agccacacgc agcagctgcc
     4261 gggaacagat tacacgattg cgggcatggt ggcttctcag tgcggcatac cgctgtttgc
     4321 cccctttgaa ggcaacgcct ccgcttctgt ttccagcttc ttcccgcaga acatctgtct
     4381 gggcgatatc ctgaaaaact cgggttatca gaactatttc gtgcagggcg cgaatctgcg
     4441 ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc gaccacttat acggctcaga
     4501 agagctgaaa agcgtggtag ccgacccgca ctatcgcaac gactggggat tctacgacga
     4561 taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt
     4621 ctcactgttt accctgacag tcgataccca tcacccggat ggttttatct ctcgtacctg
     4681 taaccgcaaa aaatatgatt ttgacggtaa accgaatcag tcattcagcg cggtaagttg
     4741 cagccaggag aatatcgcga cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga
     4801 tactgtcatc gtcgtctctt ctgaccattt agcgatgaac aacacggcgt ggaaatacct
     4861 caataagcag gatcgcaata acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga
     4921 gacgctggca gtgaagcgta acacgatgga taacggagcg acagtgctgg acattctcgg
     4981 tggcgataac tatctcggac ttggtcgtag cagtttatcc gggcagtcaa tgtcggaaat
     5041 cttcctcaat atcaaagaga aaacattggc gtggaagccg gatatcatcc gtctgtggaa
     5101 attccctaaa gagatgaaag agttcaccat cgaccagcag aaaaacatga ttgccttctc
     5161 gggtagccat ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct
     5221 gccagaaagc gaatactcag caccgctgcg tttccagctg gccgatttcg ctccacgcga
     5281 caatttcgtc tgggttgacc gttgctacaa gatggcacaa ctctgggctc cggaactggc
     5341 actttccacc gactggtgtg tctcgcaggg gcaacttggc ggtcagcaaa ttgttcagca
     5401 tgttgacaaa acaacatgga agagcaagac ggcatttaaa gatacggtca tcgacatggc
     5461 gcgttacaaa ggcaatgtcg atacgctgaa gattgttgat aacgatattc gctacaaagc
     5521 cgacagtttc atctttaacg tcgccggtgc gccagaagag gtgaaacagt ttagcgggat
     5581 ttcccgtccg gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat
     5641 cgagtacaag catccgctgc cgaagaaatt tgacctggtg attaccgcca aagcatacgg
     5701 caataacgcc agccgtccta ttccggtacg cgtaggcaat gaagaacaaa ctcttgtgct
     5761 gggcaatgaa gtgaccacca ccacactgca tttcgataac ccaaccgatg ccgacacact
     5821 ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg aatatcctcg gacactcgcc
     5881 gcgtaagctc gggatcggca tggtggaaat taaagtggta gaacgtgaag ggtaattcct
     5941 gactgatgtg taggtcgcat agggtgcgac ctacacacct tctccggctt acaacacatt
     6001 attaagaaac aaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat
     6061 aatctcgcct ttcagaaagt aattacgcct ttaattaact cacgattgtt aatcacatcg
     6121 cgctcgtagg tttctgccag cgtggcgaac ggatagcgat gggttaacat catgccagca
     6181 gtgatttttc cttccgccat cagtcgaccg actttagcaa aatcttccgg cgtggcgttg
     6241 cggctgccca tcatcgtcgt ttctttttta tggaattccg gatcggagaa ctgcaactca
     6301 cctttaaaca ggccgacaaa taccaccgtg ccgccgtgac gaatcagatt cacggtgtta
     6361 ttcatcgcat gttgattacc tgtcgcgtcg atcactttct gcgccagcga accaccaaac
     6421 tgcgcccgca gctgcgcgtc aaaatcttcg gctgacgggt ccagcacggg caactccaga
     6481 cgcgttgcca catgttcacg gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca
     6541 tcggctttag cgattgccgc cgcgcccaga ccgattggcc cggccccgac caccagcacc
     6601 tgctcgccgg gagcaatggc tgcgcgacgc accgcatgag cgctaatagc gaaaggttca
     6661 atcaatgcgg ccgcctgcgg gtcaataccg tctgcgggca aaatgttcgc caccggcacg
     6721 ctcaaatact cactaaaacc gccatcctga tgcacgccaa tgactgaaat tttttcacag
     6781 caattggtac gcccgctttt acacgccggg cattgctgac aggcaacata agggatcaca
     6841 gcaacttgct gaccattttt aagatcagca atatttttac ccagcccaac aatctcccca
     6901 catatttcat ggcctaaaac ccgtggataa ctaaaaaatg gttgatttcc accccaggca
     6961 tgaatatcgg taccgcaaat cccgacagac ttaattttta ttaatgcttc attgtcaccc
     7021 ggaataggta tctcgcgttg tttccagact aattctttcg gctgctggca aattaaaaca
     7081 ttcatcgtag acatgattcg gctctccatt ttttgttgcc ttagatatca atgaaaatga
     7141 aaaatggcag cggaacggaa aatctgtttt gtgaaaacac acgcataaaa atgttttaaa
     7201 tcgggtttta atggactcac aaggaggaaa atatgagtcg ttcacaaaat ttacgccaca
     7261 atgtgattaa ccaggtcatt gatgatatgg cgcggggtca tatcccttcc ccacttccat
     7321 cgcaaagtgc gctggcggaa atgtacaaca tcagccgcac tacagtgcgt catattctca
     7381 gccacttacg cgaatgcggc gtcttaacgc aggttggaaa cgactatgtt attgcgcgta
     7441 aacctgacca tgatgacggt ttcgcctgta ctaccgcttc aatgagtgaa caaaacaaag
     7501 tgtttgagca ggcttttttc acgatgataa accagcgcca gttacgccca ggggaaactt
     7561 tttctgaact gcaactggct cgggccgcgg gtgtcagccc ggtggttgta agagaatatc
     7621 ttttaaaatt cggccgttat aatcttattc aaagcgaaaa gcgcggccag tggagcatga
     7681 agcaattcga tcaatcctac gctgagcagc tgtttgagct acgcgaaatg ctcgaaacac
     7741 attcacttca gcactttctc aacctgcctg atcatgatcc ccgctggctg caggcgaaaa
     7801 ccatgctcga acgtcatcgg ctactgcgtg acaacatcgg caatagcttt cgcatgttct
     7861 cgcaacttga cagagatttt cactccttac tgctttcagc tgccgacaat atcttttttg
     7921 atcaatcgct tgagatcatc tcagtcatct ttcattttca ctatcaatgg gacgaaagcg
     7981 atcttaaaca gcgcaatatc attgccgtcg atgagcatat gaccattctc agcgctctga
     8041 tttgccgcag tgaccttgat gccacccttg cgctccgtaa ccatttgaat tcagcgaaac
     8101 aatcaatgat tcgatccatt aatgagaaca cgcgttatgc tcattaagta cggattaaaa
     8161 acagcaaact attagcgggt gcacggtttt atcaaaagat aaataccggg aaattcccgc
     8221 ttacctatgc tcacaatcag tcgactaaaa atgtttttgt gagcggtagt aaagtcctaa
     8281 aactttaacc tgccgttaat gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa
     8341 ttataaatac aggagtccgg cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca
     8401 tttactccat cttcgtcggc agatattccc gtgccaccag atggattagt tcaacgcagt
     8461 acccgaatta aacgcattca aaccaccgcc atgttgttat tattttttgc ggcggtaatc
     8521 aattatctcg accgcagttc gctgtcggta gcaaatttaa cgattcgtga agaattggga
     8581 ttaagtgcca ccgaaatcgg cgctttgctc tccgtgtttt cactcgctta cggcattgcg
     8641 caacttcctt gcggcccact attggatcgt aaaggcccac gcctgatgct gggactgggg
     8701 atgttcttct ggtcactgtt ccaggccatg tctggcatgg tgcacagctt tacgcagttc
     8761 gtgttggtgc gtatcggtat ggggattggt gaagcgccga tgaacccatg cggtgtaaaa
     8821 gtcattaacg actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca
     8881 gcttctacca ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg
     8941 ggctggcgcg ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg
     9001 tatatgctct atcgcaaccg cgagcacgta gaactgactg ccgttgaaca agcttatctc
     9061 aatgcaggta gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg
     9121 ttccgtaacc gcacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg
     9181 tggctgtatc tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa
     9241 agcacagggt tgatggcggc tatccctttc ctgtttggtg ctgccgggat gctggtcaac
     9301 ggttacgtta ctgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc
     9361 tgcattattg ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca
     9421 acatccatga tggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga
     9481 acatcctgct ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc
     9541 agtatccaga actttgccag cttcatctgc gcctctttcg cgccgatcat tactggtttt
     9601 attgttgata caacccactc attccgtctg gcactaatca tttgcggttg cgtcaccgca
     9661 gcgggagcac tggcgtacat cttcctggtt cgtcagccga tcaacgaccc gcgtaaagat
     9721 taaactccct atgcccgata acattttgct tatcgggcat ttttatggcg attaaaacgt
     9781 ttcccagttc tccccgctat ctgccactgc cattttacgc ggcgtagctg gcgtcacggt
     9841 ttttaccaca gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc
     9901 cacagcttcg gtcaggcgac tggcctgctc ttccaacgcg gcggcggcag cggcagactc
     9961 ttccaccagc gcagcgttct gttgagttac ccggtccatc tcagcaaccg ctaagccaac
    10021 ctgatcgata ccacggctct gctcatcaga agcagaagct atttcgccca taatgtccgt
    10081 cacgcgggtc acagcactga caatctccgc cattgtttcc ccggcgcttt cgaccagcgt
    10141 agagccaaca tccactttcc ccaccgagtc ttcaatcagg cttttaattt cacgagccgc
    10201 ctgggcgcta cgctgggcca gattacgcac ttctcccgca accaccgcaa aaccacgtcc
    10261 ttgctcaccc gcacgcgctg cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa
    10321 ggcaatgccg tcaattacgc tgataatatc ggcgattttc tgcgaactgg tggagatatc
    10381 acgcatagtc tgcaccacgt tatctaccac tttgccgccg cgttgcgccg tttcagaagc
    10441 acttaacgcc aggtggctgg cctggcgcgc attctcggcg ttctgtttca ccgttgcggt
    10501 gagttgctcc atgctggctg ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga
    10561 agagagatcg ttattgccgg tagcgatttc gctggcaccg ctatagatgg cattggcccc
    10621 gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc atatggcgca aactctctgc
    10681 cagttgcccc atctcattag agccatccac ctcaatcggt ttcaccagat cgccgcctgc
    10741 aatatgacga atgctgtcaa tcaggcgatt cattggcgct accagcgagg ctttaatacc
    10801 gaaccagacg gcgaagatga ccgccagtac gacgatcatc acgcccacca gaatccacat
    10861 cgcctggctg taggaggcat tgttatcgct gacggcgata tcatagagcc gatcgttttg
    10921 ctccatgtaa gccacatact gcttctcgaa accgtcctga tatccctggg tcggctgatc
    10981 aaagaactcg ttgattttgc ctgcacctaa cagttggatc agctccgcca gcgcattgtg
    11041 ataaatatcg taattacgtt tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg
    11101 cggcaacgct tcgtaatctg cccagttttt ttccgcctgt ttcaacgaaa tactggcact
    11161 ctgcatcagc tcagcaacgg ttgaaccgct accaatatta ttctgatcca tcatgtagcg
    11221 gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc tgccattcag
    11281 cgtggattgc tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt cattctttaa
    11341 ggcattaaag aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag
    11401 cagtaagctg gtcacgatct tgatacgttt taacatggtt tctctttcct gtggaccgat
    11461 gcaaaatttt cggcctgaaa tgagaaaact ttataggtag cgacttgaaa ctggttaatt
    11521 ttacctcact ttttttaatt tttgttcaaa aatacaataa ccacttattg atacattttc
    11581 ttttcttatt tttttcacac gaattgtatc tattttgaac gaaatatttt atagagtgtg
    11641 gaattttaag aaaggacatc tgaaatgtta aaccgtgact tcgatcatct ctcaacgcta
    11701 ttacatctct tttttgaacg tgaggatatt tgccaaaagc ttaatattat tgaccaaaac
    11761 aatatcaccg gttgggaagt gtggttccag gtagagtttg ctaatatgct ctgctcaaca
    11821 gaccatgaat ggtggcgtga acaagcactt tcctgtgata tgcgcaaaaa acctgagcgc
    11881 ccgacactac gcacagattt cttactacgt aaaaaaggct gggcacaaga tagctatatc
    11941 gcgcttgaaa taaaacaaaa cagagacgcg accagttgtg ttaaaaatat gatcgcagac
    12001 ttagaaaaaa gcgcaaaaat aaaacgttcc gaactcgatt tacgcagttt ctggaccttg
    12061 ggtatcaccc ctatgataga cagggaaagg ctggactctc tcatcgattg ctatttagat
    12121 gagaaatttt atcttactaa atctcgtaaa aaacacgttt tac
//
LOCUS       DAZGXS010000122        12071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGXS010000122 DAZGXS010000000
VERSION     DAZGXS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..617
                     /locus_tag="ACHKG5_003737"
     CDS             <1..617
                     /locus_tag="ACHKG5_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0869751.1"
                     /translation="LSGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELMVSIRPMQVN
                     GYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEMLENMDLLSN
                     YQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMNSNVCMLYDK
                     MALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            648..782
                     /locus_tag="ACHKG5_003738"
     CDS             648..782
                     /locus_tag="ACHKG5_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFP0869752.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLVGELSIVLVLDDFQPLPILRAN
                     "
     gene            complement(779..1027)
                     /locus_tag="ACHKG5_003739"
     CDS             complement(779..1027)
                     /locus_tag="ACHKG5_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0869753.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(1255..1320)
                     /locus_tag="ACHKG5_003740"
                     /pseudo
     CDS             complement(1255..1320)
                     /locus_tag="ACHKG5_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1542..3227
                     /gene="btsS"
                     /locus_tag="ACHKG5_003741"
     CDS             1542..3227
                     /gene="btsS"
                     /locus_tag="ACHKG5_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0869754.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3224..3943
                     /gene="btsR"
                     /locus_tag="ACHKG5_003742"
                     /gene_synonym="yehT"
     CDS             3224..3943
                     /gene="btsR"
                     /locus_tag="ACHKG5_003742"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0869755.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3990..4460
                     /gene="yehS"
                     /locus_tag="ACHKG5_003743"
     CDS             3990..4460
                     /gene="yehS"
                     /locus_tag="ACHKG5_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0869756.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4502..4963)
                     /gene="yehR"
                     /locus_tag="ACHKG5_003744"
     CDS             complement(4502..4963)
                     /gene="yehR"
                     /locus_tag="ACHKG5_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0869757.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5088..7091)
                     /gene="yehQ"
                     /locus_tag="ACHKG5_003745"
     CDS             complement(5088..7091)
                     /gene="yehQ"
                     /locus_tag="ACHKG5_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP0869758.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANDQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(7088..8224)
                     /gene="yehP"
                     /locus_tag="ACHKG5_003746"
     CDS             complement(7088..8224)
                     /gene="yehP"
                     /locus_tag="ACHKG5_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0869759.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(8217..10496)
                     /gene="yehM"
                     /locus_tag="ACHKG5_003747"
                     /pseudo
     CDS             complement(8217..10496)
                     /gene="yehM"
                     /locus_tag="ACHKG5_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(10507..11595)
                     /gene="yehL"
                     /locus_tag="ACHKG5_003748"
     CDS             complement(10507..11595)
                     /gene="yehL"
                     /locus_tag="ACHKG5_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0869760.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2918 a         3010 c         3154 g         2989 t
ORIGIN      
        1 ttttatctgg ggttagtttt ttgtctgaca ttcctcaaga aaccctgtct gagatacgta
       61 atcagactat tcgtggagaa gctcaaataa gactgggtga gttgatggtg tcaatacgac
      121 ctatgcaggt aaatggatat tttatgggaa gtcttaacca ggatggttta tcgaatgata
      181 atatccagat tggccttcaa tatatagaac atattgaacg tacacttaat catggtagtt
      241 tgacaagccg tgaagttaca gtactgcgtg aaattgagat gctcgaaaat atggatttgc
      301 tttctaacta ccagttagag gagttgttag ataaaattga agtatgtgca tttaatgtgg
      361 agcatgcaca attgcaagtg ccagagagct tacgaacatg ccctgttaca ttatgtgaac
      421 cagaagatgg ggtatttatg aggaattcaa tgaattcaaa tgtttgtatg ttgtatgata
      481 aaatggcatt aatacatctt gttaaaacaa gggcggctca tcctttgagc agggaatcaa
      541 tcgcagtttc aatgattgta ggaagagata attgtgcttt tgaccctgac agaggtaact
      601 tcgttttaaa aaattaaggt agcaatggcg ggttaacttt tcgtaaaatg aaattaattc
      661 tcggtacggc atgtaaaacg cggcacctcg tatgcaagaa cgtactgcgg ttggttggtg
      721 aactttcgat agtgctagtc ttggatgatt tccagccgtt accgatttta cgtgctaatt
      781 agtgaacaaa ccactcgtca gcagacttcc aggtatcttt cagagtctcc tgaacaaaag
      841 ttttagctga atctttatcg gcggtgcgcg taacagaaag gccatcgttg ctggtggctt
      901 ttacgatcac ctctacatcg tcataacgtt tactgatgcg tcgggttaat tcttccttta
      961 acgcatccac agcaccgttt ggcattttag tgattttttc tttagcgata cagatctcaa
     1021 cacgcataac accctcctgt aactgtagtt atgtacaggt gttattttta tctgtatgga
     1081 taaccagtgt caattcctga tattgtttat ggggcatcaa tggggcatgt atgggacact
     1141 ttttatcggc gaaattcgtc gaagttcatc gacatggtaa acgaatcatc tatccaaccc
     1201 ttgaaaaacg gcgctcctgg acgatcttcg tcgattttta aaaatgttgc gtcacgcgcg
     1261 taacgtgaca gggttaatat cacaaagcaa cgccacttca ccaattgtgt aaagcgccat
     1321 cgtctcaccc ttgctcgcga ggtcccggtt taactttaga cgcagttttg cgaaccaggt
     1381 agttttgccc gttttttgtg catttatagg gtgattttat ttttgccagg cgattttgag
     1441 tgatcgtact cacgaattct catttttctg caagagttca aagaaagtta aacgcaggca
     1501 atgtatgtta cgcgttttaa agggatgtgt ggtttgcggg tatgtacgat tttaatctgg
     1561 tgttgctgct gcttcagcag atgtgcgttt ttttagtcat tgcgtggtta atgagtaaaa
     1621 cgccattatt cataccgtta atgcaggtca cggttcgtct gccgcataaa tttctctgct
     1681 acatcgtctt ttccatcttc tgcatcatgg gcacctggtt tgggttgcac attgacgatt
     1741 ctattgccaa tacccgtgcg ataggcgcgg taatgggcgg cttactcggc ggtccggtcg
     1801 tcggtgggct ggttggcctg accggcgggt tacatcgata ttcgatgggg ggcatgaccg
     1861 cgctaagttg catgatctcg accatcgttg aaggattgct cggcggcctg gtacacagca
     1921 tcctgatccg tcgcgggcgc actgataaag tctttaaccc cattaccgct ggtgccgtca
     1981 cgttcgtcgc tgaaatggtg caaatgctga tcatcctggc gatcgcccga ccttatgaag
     2041 atgcggtgcg tctggtgagt aatattgctg cgccaatgat ggtcaccaat accgtcggcg
     2101 cggcgctgtt tatgcgtata ttgctcgata aacgcgcgat gtttgaaaaa tacacttcgg
     2161 ctttttctgc cactgcgctg aaagtggcag cctcgacgga aggcattttg cgccaggggt
     2221 ttaacgaagt gaacagcatg aaagtggctc aggtgctgta tctggagctg gatattggtg
     2281 cagtcgcgat taccgatcga gagaaattgc tggcctttac cggaattggt gacgaccacc
     2341 atttacccgg caaaccgatt tcttcaactt atactttaaa agcgattgaa accggtgaag
     2401 tggtctacgc tgatggcaac gaagtacctt atcgttgctc tttgcatccg caatgcaaac
     2461 tggggtcgac gctggtaatt ccgttgcgtg gtgaaaatca gcgggtgatg ggcaccatca
     2521 aattgtatga agccaaaaac cgtttattta gttcaatcaa ccgcacgctg ggcgagggga
     2581 ttgcgcaact gctttcggcg cagatcctcg ccgggcaata tgagcggcaa aaagcgatgc
     2641 tcacccagtc agagatcaaa ctgcttcacg cccaggtgaa cccccatttt ttgtttaatg
     2701 cgcttaacac cattaaagcg gtgatccgtc gcgacagcga acaggccagc cagctggtgc
     2761 agtatctttc cacttttttc cgcaaaaact taaagcggcc ttcggagttt gttactctcg
     2821 ccgacgaaat tgaacatgtg aatgcttatc tgcaaattga aaaggcgcgc ttccagtcgc
     2881 ggttgcaggt caacattgct attccgcaag aattatccca gcagcaattg cccgcgttta
     2941 ccctgcaacc gatagtggaa aacgccatta aacatgggac atcacaactg ctggatacag
     3001 ggcgagtggc aatcagcgtc cgacgtgagg ggcaacattt gatgctggag atcgaagaca
     3061 atgccggttt gtatcaaccg gtaaccaatg ccagtgggct ggggatgaat ctggtggata
     3121 agcgtttacg tgaacggttt ggcgatgact atgggataag cgtcgcctgt gagcctgata
     3181 gttacacccg aataacgtta cgactaccat ggagggacga ggcatgatta aagtcttaat
     3241 tgtcgatgat gaaccgttag cacgggagaa cctgcgcgta tttttgcagg agcagagcga
     3301 tattgaaatc gttggtgagt gttcaaacgc cgtggaaggg atcggcgcgg tgcataaact
     3361 gcgcccggat gtgctgtttc tcgatatcca gatgccgcgc atcagtggtc tggaaatggt
     3421 ggggatgctc gacccggaac atcgtccgta tattgttttt ctcaccgcgt ttgacgaata
     3481 cgctattaaa gcctttgaag aacatgcctt tgactatctg ctgaagccaa ttgatgaagc
     3541 gcgactggag aaaacgctgg cgcgattgcg tcaggagcgc agcaagcagg atgtttcgct
     3601 gttaccggaa aatcaacagg cgctgaaatt tatcccttgt acggggcata gtcggattta
     3661 tttgctgcaa atgaaagatg tggcatttgt cagcagtcgg atgagcggtg tctacgttac
     3721 cagccacgaa gggaaagagg gctttaccga attgacatta cgtaccctgg aaagtcgtac
     3781 accactactg cgctgccatc gtcagtatct ggttaacctc gcgcatttac aggagattcg
     3841 tctggaagat aacggtcagg ccgagttgat tttgcgtaat ggcttaaccg tgccggtcag
     3901 ccgccgttat ctgaaaagct taaaagaggc gattggcctg taaaagactg ctaaaatggc
     3961 tttttgcctc atcaacacct gaaggcctca tgctaagtaa cgatattctg cgcagcgtgc
     4021 gctacatttt gaaagccaat aataatgacc tggtgcgtat actggcgctg ggtaatgtcg
     4081 aagccaccgc ggaacagatc gccgtctggc tacgtaaaga agacgaagag ggttttcagc
     4141 gttgtccgga cattgttttg tcgtcattcc tcaatggcct gatttatgaa aaacgcggca
     4201 aggatgagtc tgctccggca ctggagccgg aacgtcgcat taataacaac atcgtgctga
     4261 aaaaattacg catcgcgttt tcgctgaaaa ccgatgacat tctggcgatc ctcaccgaac
     4321 agcagttccg cgtttcgatg ccggaaatta cggcgatgat gcgtgcaccg gatcataaaa
     4381 acttccgcga atgcggcgat caatttttac gttattttct gcgtggactg gcagcgcgcc
     4441 agcatgtgaa gaaaagctaa gacgggtatg gcggccatgc gcaaacatgg ccgccgacag
     4501 attatttcac ttctttaaaa ccagcggctt tcatcaccag ttccatttgc gccatagtga
     4561 tacctttttt ggcatcttca gcagaaacgt tgattcctga aataccctgc agggctttaa
     4621 aatccacttt ttccatatcg atagtcacgt tttcctgcgc gtaggtatcg gtataggtta
     4681 atttttcttc aacacccgcg atgtttttgt atttggcgct taacggctca agtgtcttgg
     4741 cagcatcttc tttggtggta gcaccaatgg aggcaaattg aattttggtt tcagaagatt
     4801 gcttaagcac cttgtcacct ttgtagacat aggtaatggc aatttcagtg ccgttcagat
     4861 tggcgctgaa tttcttcgat tcttctttgt caccgcagcc agcaagagaa aaaaccagaa
     4921 cagatgcaac aacaagggaa aacagcttat tgaaagcctt catgtaaaac tccattttat
     4981 ttaatcaagg aactggtgac tctcaccagg ggctatatag gatatgccta ataccgtggc
     5041 gtgagcagtc cggaactgga gtagaactct tagtaaaaag cactatttca tccttgttgc
     5101 tgaagcaggg ggaataattg ttcgcacagc aaaacaccgt tattcattgc ttctacccgt
     5161 gcctcgcttt ctgtattacg aaattgtccc aatacatgtg ccagccgata aaaacccacc
     5221 gcggtgaggt cattcgccag caactctgcc tgaccaatag cactctgttc ctgatagcgc
     5281 cagccgttat ggagcagttg aataagtaat gcctggcagc gcatcagcaa ctgatgagca
     5341 gtagacggca caggcagaac gctggcagaa ggtagtgatg ccaccacagg cgcagtttct
     5401 gcgtccagcg cccaggcacg agtctttgtc atcattacct gtggttccag tgtcaattgc
     5461 ccatcaacaa aactgacaaa gccagaaacc agacacacgg ggtcgtctgt ttgttgcaaa
     5521 agcgccgcca tgcgttcaac ggcataaggc gcgctggctg aggctggcag tgataacgtc
     5581 agcagattat cttcaccttc gccgctgatt acctgcgcat ccagcgtctg gcggctgcta
     5641 tcccaaccga gcgaaataca ctcagcgacc ggcagaataa ataagttatc gacctgatta
     5701 agaggccgta tgcaggaggg gggacgctgg cgtaaatatt cccgcaaagc cacaatgccc
     5761 ggctggcgta acggcgcgct caacattcgc caggcatcag gcgacagcgg cacaacgctg
     5821 cttaagcggt tgcgggtagc taacagcagc tcgccatcgg cactgcgttt tgctgcttgt
     5881 gaaacaattt gcccacccgc cagtgcgcca gcctgaaaac taaacagccg acgcgtagct
     5941 gccggtgagt tttcctgttc acttcgcggc caactgcgcg aaaggtgcaa aatactgccg
     6001 gtgtcgggat cggtaaacca gatgcgtaaa ccataatgct caatatcctg ccagcaacgc
     6061 atacctaaag acaccagccg cagatgatca agctttgctt ctccggcaat gccagagcca
     6121 acgaccgtgc gccacggcac aggaggaact tcaccaacac tgtcgcgccg ggccatctct
     6181 tgtgcgcaat ttaatcgact gtttaatgcc gcaagctgac gtaagcattc tcctgcatga
     6241 tagtggctgg cgcgggcgtg gaaggcatca acgctggcgc gcagttgccg tagcgattca
     6301 ctcacccagc gccagttgca gcgttccgcc gcttgctgcg cgcggctgaa agcggcctcg
     6361 tagtgaataa gcggctggct gatgccgcca agccataatg cctggcttaa ttgctgaaca
     6421 tattgacgac acgcgttgcc ttcttcgctg gcaaacgggt cgtcagatga tgtgacgtgt
     6481 tcgctgcgca tctgccagat taaatgagta aattccgctt gctgagtttt ggcctcgacg
     6541 aaggcctgca cagccagtac gacatgttcg caaagtgtgc cttcaataca atcacagcgg
     6601 gcgaagcgaa tgctgctgcg ggaataaaaa cgcacatcac tcatcggtaa gcgggctgag
     6661 ggaatttcgc caggcgcaca gaacaactca atggtgatgc ctttagcgac cagcacctgt
     6721 gcacgtttgc gggtggcatc gggcagggta gccagttctt ccagccagat tgcaggctgc
     6781 cactcttctt ctttttccgt gggccgggtg gtggcacaaa gtcgctgata acttaacacc
     6841 agcatcacgc gatgacggca catgccgcta gccccgcagg tgcactgagc ctctttcagt
     6901 gcctggtcgt tcgccagctg ggtacggaca ccgtcactga aggtggcgat taaagcgccg
     6961 ttctcatggc tgatttccgg gacgttgcca ttttccagtt ccttaaggct gcgcttaaca
     7021 aaaccggcat tgcttaacgc cgtcagggcc tgcggcgtga gttctaataa ttccggacgt
     7081 agtgaattca tgactgcaaa ttctccgcaa gccaggtagc cagttcaccc ggtgtcattg
     7141 cggctatttg tgcgccaaca ttaaccagcg cctgggccat atcgcggtca tagcaaggcg
     7201 ttgcggtgct gtcgagcgct gccagcccca gcactttgat gccgctctgg acacactttt
     7261 tcacctgatg cgtcagcaat gatgatgaac ccccttcata aaaatcactc acgaggataa
     7321 tgacgctttt tgctggttgt tcaataagtt gccgcccata ctccacggca ctggcgatat
     7381 tggtcccgcc gcccaactgt actttcatta ataactctac cggatcggca acgtctgccg
     7441 tgagatcaac gacactggtg tcaaacgcca ccagatgggt acgaatgccg ggtaactgcc
     7501 acaaacaggc cgccattacc gcagagtgga tcaccgaatc gaccatcgat ccgcttagat
     7561 caaccagtaa gaccagttgc cattgttcac tgtggcgctt aattcggctg ttaaagcggg
     7621 gggattcgat atacaacttg ccgtgttgcg ggtgccagtg ttgcagattg gcgcgaagag
     7681 tacttttgaa atcaaagtct cgcgccagtg gaatagatga gcggcgacgg cgatcgcgga
     7741 caccagaaaa agcctgacga acttcctttg ccagtcgagc cataatttct tcaacaacct
     7801 ggcgcactat ccggcgggcg gcagccagta cttcggggtt catcagatgt ttggtgtgca
     7861 aaacggcgcg tagcaggctt tcagaaggct gcatacgttc cagcacgtcg agattcgtca
     7921 ccacatcttc aatgccgtag cgcagtacgg catcgctttc cagccgctca atcacctgtt
     7981 gcggaaacag cgtgtgaata ctgttgatcc actcaggggt ggtgagattt gagccaccta
     8041 atccaccgga gcgttcacca cgctggagcc gttcaggatc gcgcccatac agccactcca
     8101 gcgcgtggtc tatctgccgg gcgttgtcat ccagcccaca aagcgtcgtt tctgccgctt
     8161 cgccaagaat taatcgccag cgttgtagct cacgggtggt cagaagatcg ttcagttcag
     8221 acatggaaaa ctccccagtt ttgcagcgat gccagtgcct gctgttcgag ttgttgatga
     8281 tgtgcaatgg cttgtggtgg gcaatgcaac ggcatttgca gcgccgaaac gggaagttgc
     8341 gccagttgat aatgttcaag cacctgatgc gccagtgtcc cgcgttctcg tggcggtagc
     8401 caggccatcg ccgcgcgtaa atcgggcagg gcattgataa aatcggcatc acttggttga
     8461 tttaaatgac tgctgaaacc ggcgataaat gccggctgac aggccagttg atggcgggcc
     8521 agcgccagca aaccgtgcag cgcttcgccg gattgtgccg gggataactg cgccagcatc
     8581 gtcagcgccg cttcggcact agcgttggga tgttccagac gcatcagtgc gccaagagcc
     8641 gcgccgcgat ccagcgccgg ggcatggatt gcctgactgc gtcgctccag taaggctacc
     8701 gccgcagaaa gagaaacgcc gggtaaatta acgccgctat gtagatcgcg cagaatatgg
     8761 caaagcgctt gccagctatg caggtgagcg tgaaactcct tttcatccgg tctgccgtta
     8821 gattcacaca gccacagcgt gcgatcgata gccgcgcata acgtcgtttg tgatatctgc
     8881 gcaccttgca taccgctaat ttcatccagc cgccataagg catatagcac ttccagcgcc
     8941 gggcccattt cggcaaattg attttcctag gcgattaata atgtgagttg ctccagtaat
     9001 tgttgactga aggacgccag acccgctaac gccgcctggc taagacatgt ggtgatactg
     9061 ccgattccac ccgcgtccag catatcggct tctaatttat ggcgtgcggc ttcctggagt
     9121 gtggcaccaa agcaggcggc ctcaatcaat gccgcatgct ggctaagcgg gcgggttaat
     9181 ttccagcgtt cttcaccgtt gcctgcaagt gtcagtgtgc ttccctgctg gcgtacaatc
     9241 cctgggatct ccagtattgc cagccgatgt aacacctgac tttgcgctag tccattgggg
     9301 ttaaagcgat ttaaggtaag ctccgccgaa agagagattg cggtacgttc cagttcgcag
     9361 gtgacatctt ttggcagagg cggttgtggc gtagaagggg caagtttacc gaatccgtca
     9421 cccgctaatg tgtctatcaa cgttagcaga atcggatcgg tatcgggatg aataacgccg
     9481 cgatagctcc acggcaacgg cgcgttcagg gcttctttaa tcagcgagcc tgctatggca
     9541 tccagccagt cagtgcgtaa cggtagttta tatccgcgca actgtgccag cgccatcgca
     9601 tgcagatgag cggcagccat atccgcggtc gaagcaggca agtggtgttg gcgcaaacgg
     9661 gtaagaaccg tttttagcag ttgttctccc gcctgctgta agccccactg ccagcaccag
     9721 ctttgccaga ccggggcagg cattcctgaa aggtatcctg ccagcacatc aaggcgcttt
     9781 tcactgtagg gtgtgagata acaacctgta acggcatctg ccagcgaggg caattctggc
     9841 ttgttaatgt cctgcgggca ttcgcgccac atttttgcca gtgccggagc gtgccagcca
     9901 ccgcagacga ctaacacgtc gccattattt tgctgcactg cccatgcaat ccagcgagcc
     9961 ataaaggctt cgcgctgacg attgagtgca tcgccgggga aatcgccccg cagttgggca
    10021 aaatagtgcg ccagtgcaga gggtaatgca gtttgctggc tttcatcttc gaacaagtga
    10081 tcccacaggg tatcgctgtt atccatgcgg gtggcacgca gcagcaaggc ctggctttcc
    10141 tcttgcgtat caggcgaatc gtcctcttct tcactctgcg cccagcaagg caaatcgata
    10201 aagtaagttt gtgcctgaat atgacgtgcg gcttgtagcg cctgccactc cggcgaaaat
    10261 tcggcaaatg gcgtccaggc gccgcgccca ggagcagcac cgtcctgata ctggcaataa
    10321 ctgtaaatag ccaccggaag ctggtgggct aaaaacagtt catctacccg gtcattaaaa
    10381 tcagccgggc cttcaatcaa cacgtatcgt ggtcgctggc tttcgattaa cgatttcacc
    10441 agtcgggcgc aggccggact atgatgccgg atgccgacaa ttaacggctc gctcatgcat
    10501 ctttcctcac ggcaggcggt ggcgggcttg ataataagcc tgccagtgag cttctttatg
    10561 tgtggcgaca cgctgttcga agtaacggcg cagacgagcg cgatcttctt cgttatcttt
    10621 gacgatggtt ccggcaatac agtccaccag gtcggcaggc tcccccgcgc gattcgctaa
    10681 aaaccaggcg cggacgccca cagcgtgggc cacgttcacg gcttcagcgg tggacataat
    10741 cgctgttagt gtatccattg acgttttctt ttcgccgttg gcacgcagat cgcggaaggt
    10801 gcggaccagt aactccagta ccgcgtcagg cactttatgc ggaataccgc tatgggccag
    10861 caaacgcgcc gaagcactgg cgacaagctc cagctcctgg gcgaaatcca taatcggaaa
    10921 gacagtttca aaatcgaagc gacgctttag cgcagcactc atttcattga cgccgcgatc
    10981 gcgggtattg gcagtggcaa taatattaaa gccttctcgc gcatagagct gactggcttc
    11041 accagtgagt tccggcaccg tcatcaccct gtcagaaagc atacctaaca gacagtcttg
    11101 cacttccagc ggtgtacgcg ttatctcttc aaaacggacg attttgccgt cgcgcattcc
    11161 ctggtaaagc ggggcaggga ccagcgcttc cgttgatgga ccatgattga taagcagggc
    11221 gtagttccag ccgtacttga tttgatcttc ggtagtagat gcgccgccct ggatggttaa
    11281 cccggcatcg ccgctaatag aggttgccag taattcagac aggagagatt ttgcggtgcc
    11341 aggttccccc accaacatca aaccgcgtcc ggtggcaaga gtgactaaca tacgttcgac
    11401 agcggaggga ctaatcacca ctttacggct aatattttgc gcgctatcgc caaggataaa
    11461 agcacgggcc gcaggcaaac ttaactgcca accgggcggg caaggtgcgt tatcattttg
    11521 ttttaatttt gccagttcat cggcgtataa cacagcagca ggcggacgct gcagatgatt
    11581 attctgcggt gacatatatt catccctgaa atgtattaat gaaataaacg atattttacg
    11641 cgcaataaat gtttggcgca ttagttgttt gagcgaaaaa tatctgggaa gcagatcaca
    11701 gtaaataaaa aaacctgctg gtgaggcagg ttcttttatt tactattttc aggcctaaca
    11761 ccacagcctg tgcaacatcg tgttccaacg aacgaattat aaccacggct ggaccccgtt
    11821 gcaattaaga taatgagatt tataatcctg actgttggga cgttgcgccg gatcgaacct
    11881 atttcaagtc ctggctaccg gacggggggg gctgcaggtg gtgccctgat ccggagtgca
    11941 acatcgtggt ccaacatacg taaaccagta tcgacaggca gtgtaagggt aaggtgctgg
    12001 tgttgcgctc cttaataagg agagcggatg gactcgctga cacaaaagtt aaccgtgctc
    12061 attgctgtac t
//
LOCUS       DAZGXS010000123        11889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGXS010000123 DAZGXS010000000
VERSION     DAZGXS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            174..824
                     /gene="csgD"
                     /locus_tag="ACHKG5_003749"
     CDS             174..824
                     /gene="csgD"
                     /locus_tag="ACHKG5_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0869761.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            829..1218
                     /gene="csgE"
                     /locus_tag="ACHKG5_003750"
     CDS             829..1218
                     /gene="csgE"
                     /locus_tag="ACHKG5_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0869762.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHSVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1243..1659
                     /gene="csgF"
                     /locus_tag="ACHKG5_003751"
     CDS             1243..1659
                     /gene="csgF"
                     /locus_tag="ACHKG5_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0869763.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1686..2519
                     /gene="csgG"
                     /locus_tag="ACHKG5_003752"
     CDS             1686..2519
                     /gene="csgG"
                     /locus_tag="ACHKG5_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0869764.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2583..3074)
                     /gene="ycdZ"
                     /locus_tag="ACHKG5_003753"
     CDS             complement(2583..3074)
                     /gene="ycdZ"
                     /locus_tag="ACHKG5_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0869765.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3176..3730)
                     /gene="ycdY"
                     /locus_tag="ACHKG5_003754"
     CDS             complement(3176..3730)
                     /gene="ycdY"
                     /locus_tag="ACHKG5_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0869766.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3754..4491)
                     /gene="ycdX"
                     /locus_tag="ACHKG5_003755"
     CDS             complement(3754..4491)
                     /gene="ycdX"
                     /locus_tag="ACHKG5_003755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0869767.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4546..5484)
                     /gene="ghrA"
                     /locus_tag="ACHKG5_003756"
     CDS             complement(4546..5484)
                     /gene="ghrA"
                     /locus_tag="ACHKG5_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0869768.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5718..5805
                     /locus_tag="ACHKG5_003757"
     tRNA            5718..5805
                     /locus_tag="ACHKG5_003757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5752..5754,aa:Ser,seq:gga)
     gene            complement(5955..6797)
                     /locus_tag="ACHKG5_003758"
     CDS             complement(5955..6797)
                     /locus_tag="ACHKG5_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0869769.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6882..7079)
                     /locus_tag="ACHKG5_003759"
     CDS             complement(6882..7079)
                     /locus_tag="ACHKG5_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0869770.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7091..7582)
                     /locus_tag="ACHKG5_003760"
     CDS             complement(7091..7582)
                     /locus_tag="ACHKG5_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0869771.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7579..7953)
                     /locus_tag="ACHKG5_003761"
     CDS             complement(7579..7953)
                     /locus_tag="ACHKG5_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0869772.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8000..8374)
                     /locus_tag="ACHKG5_003762"
     CDS             complement(8000..8374)
                     /locus_tag="ACHKG5_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0869773.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8537..8758)
                     /locus_tag="ACHKG5_003763"
     CDS             complement(8537..8758)
                     /locus_tag="ACHKG5_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0869774.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8821..9297)
                     /locus_tag="ACHKG5_003764"
     CDS             complement(8821..9297)
                     /locus_tag="ACHKG5_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0869775.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9313..9798)
                     /locus_tag="ACHKG5_003765"
     CDS             complement(9313..9798)
                     /locus_tag="ACHKG5_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0869776.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9889..10707)
                     /locus_tag="ACHKG5_003766"
     CDS             complement(9889..10707)
                     /locus_tag="ACHKG5_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0869777.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11047..>11889)
                     /locus_tag="ACHKG5_003767"
     CDS             complement(11047..>11889)
                     /locus_tag="ACHKG5_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP0869778.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT         2987 a         2998 c         2952 g         2952 t
ORIGIN      
        1 agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt
       61 tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct
      121 attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc atcatgttta
      181 atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaacct tctttgcagg
      241 cgacagctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa ttacataata
      301 ttcaacgttc tctggacgat atctcttcag gctctattat tcttctggat atgatggaag
      361 cggataaaaa gcttatccat tattggcagg atactttgag caggaaaaac aacaatatca
      421 aaatattgtt gctaaatacg cctgaagatt acccgtaccg cgacattgaa aactggcctc
      481 atatcaacgg cgttttttat gccatggagg atcaagaacg cgttgtcaat ggattgcaag
      541 gcgtcctgcg cggcgaatgc tactttacgc aaaagcttgc cagttacctg attacacatt
      601 ctggtaacta tcgttataac agcacggaat cagccctcct tactcatcgg gaaaaagaga
      661 tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca
      721 gcgaaaatac ggttaaaacg catctttata atcttttcaa gaagatagcc gtaaaaaacc
      781 ggacacaagc ggtttcctgg gcaaacgata acctcaggcg ataaagccat gaaacgttat
      841 ttacgctgga ttgtggcggc agaatttctg ttcgccgcag ggaatcttca cgccgttgag
      901 gtagaagtcc cgggattgtt aactgaccat tctgtttcat ctattggcca tgatttttac
      961 cgagccttta gtgataaatg ggaaagtgac tatacgggta acttaacgat taatgaaagg
     1021 cccagtgcac gatggggaag ctggatcaca ataacggtca atcaggacgt tattttccag
     1081 acttttttat tcccgttgaa aagagacttc gaaaaaactg tcgtctttgc actgattcaa
     1141 actgaagaag cactaaatcg tcgccagata aatcaggcgt tattaagtac gggcgatttg
     1201 gcgcatgatg aattctaaat aaaaaattgt tcggaggctg caatgcgtgt caaacatgca
     1261 gtagttctac tcatgcttat ttcgccatta agttgggctg gaaccatgac tttccagttc
     1321 cgtaatccaa actttggtgg taacccaaat aatggcgctt ttttattaaa tagcgctcag
     1381 gcccaaaact cttataaaga tccgagctat aacgatgact ttggtattga aacaccctca
     1441 gcgttagata actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat
     1501 attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac
     1561 cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc
     1621 caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa
     1681 taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg
     1741 ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc tacaaagatt
     1801 tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg
     1861 aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg
     1921 ccacggcgat gctggtcacg gcattgaaag attctcgctg gtttataccg ctggagcgcc
     1981 agggcttaca aaacctgctt aatgagcgca agattattcg tgcggcacaa gaaaacggca
     2041 cggttgccat taataaccga atcccgctgc aatctttaac tgcggcaaat atcatggttg
     2101 aaggttcgat tatcggttat gaaagcaatg tcaaatctgg cggggttggg gcaagatatt
     2161 ttgggatcgg tgccgacacg caataccagc tcgatcagat tgccgtgaac ctgcgcgtcg
     2221 tcaatgtgag taccggtgag atcctctcct cggtgaacac cagtaagacg atactttcct
     2281 atgaagttca ggccggggtt ttccgcttta ttgactacca gcgcttgctg gaaggggaag
     2341 tgggttacac ctcgaacgaa cccgttatgc tgtgcctgat gtcggctatc gaaacagggg
     2401 tcattttcct gattaatgat ggtattgacc gtggtctgtg ggatttgcag aacaaagcag
     2461 aacggcagaa tgacattctg gtgaaatacc gccatatgtc ggttccaccg gaatcctgac
     2521 agataaaaat gccggaatta ataatccggc atttactgtt taagcgaaag cgtgagtgcc
     2581 cctcacgctt tatttttgat ttcctgctca cggagagccg tctttgcact acgcgccgcc
     2641 agccacaggc cgctgttttt cattgcgtaa ccaaatatca gcccaagcgc cagcgaaggt
     2701 aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc
     2761 ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc gacaataccg
     2821 gttatgacat aactgagaat ttccagatgt ggcgccagtg cactaccgta aataatgacc
     2881 atcgcccaca ccacgccact aagcagagtt gcagcggaga tcgccagccc tttcagccca
     2941 ccttgcgggc aggcaaagta ggccgtacag cccaggaagc ccgcccagct tagtaagccc
     3001 agggaaacag ccacccatcc ccagataccg gagagaatgc ctgttgtgat tgcaatagag
     3061 agaagtatgt tcatgccgcg catgatagca gaaaacgcgg ccatgaatga gatcgagcac
     3121 acattttatg caacagcaaa ttatctgttg cagttatgag tgacgcatat cacatttact
     3181 cttcagaatc ttcttccagc tcgtcccaca ttgcactaat ggcatcgcgg gttagcggtg
     3241 ccatggtgcg ccagaaaggc gtggttgcat gggcctccac tttgccaagg aacgcaccac
     3301 accagggtaa cagatactca ctgaacagtg tttccagtgc ttcgctctca tcttccgttg
     3361 actgatcttc cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg gcaggcgtat
     3421 ccgctaatgg catccctcgt tcagaaagaa aagcgcgcac ttccgcttcc gttgcgtcct
     3481 caacccatgc gctacgatat ggcggcacgg cacattcatc gccgataaac aacgcattgt
     3541 aatcggcaga gacttgcgtc atatcacagc ttttctgcaa acgtgtcagc aactcatcct
     3601 gctccagtgg ccagttcgca gccagtttcc cctcacgaat cagggtaaac agcggcacca
     3661 gtaaaggatc ttgcggttgg cggtaataga gcgaacccag cacacgacag aggatagaaa
     3721 actcgttcat taatatattt ccattactag taattaaagg tctgcaaatt ccgcaatcgg
     3781 tgccataccg cgagattcaa ggaagttcag taagcggcgc ggagaaacat tcaaaatgcg
     3841 ctctggcgga aaatctaccg cgtcgaggat tttaagacac tcttcaaatt cccccatggt
     3901 aaacgcggtg tgagaatccg agcctaatgc cacccaacct ccagcatcac gtaccgctgc
     3961 ggctaccgca cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg aggaattatt
     4021 gatttccagc gccacctgat gtttcgcggc tgcttcagca acggctttaa catctatctc
     4081 atatttcgga tttccgggat ggcttattat atgcacattg ccgctggcga tagtggcgat
     4141 catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa accggctcat gaaaaccggc
     4201 aataatcaga tccagcgagt caaacatttt accgctgcaa tcaatttcac catcaacatt
     4261 tttaatgtta gcttcgatgc cgcgcaggat ccctacccca tcaaccactc gcggccagat
     4321 acgcatgtta atgaagtgcc agtgatgcgg cgcatcttcc atgtccgggc catgatcggt
     4381 aatcgcaaaa agtttaatgc ccttttgttt ggcctgggca atgtaatcac tcaatgtgct
     4441 atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg acgggataca taactctctc
     4501 ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg ctttattagt agccgcgtgc
     4561 gcggtcgact tgcccgcaga ccctctcccc tttttcgagc tgggcaatag tgcgagaaat
     4621 gtactccaca gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg
     4681 tggatgttgc cagagcggac tttcaggcgg taagggttca cgattaaaaa catccaacat
     4741 tgcgccttta actttgccgc tatccagcgc cgcgagcagg tcatcttcca cgacatgaac
     4801 accacgcgcc aggttgagga gatacgcgcc atccggtaat ttttcgagta attgttgatt
     4861 aataatgccg acggtttcag gggtattcgg taacaaatta atcaataccc gacattggct
     4921 cagaaatgca gacagttctt cccatccggc aaagctttgc acgccaggcc acgatttacg
     4981 ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc agactctgag caactttact
     5041 gcccagtacg cctgcgccca aaatgccgat ggtaaaatct tcccgatgat attcaggcag
     5101 cggttgccaa tgcgaactat tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg
     5161 cagcacctga ctgacagcat attcctgcat ttgctcgccc ataccggtat cttccaggcg
     5221 aaaaagtgga acagaaggct tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga
     5281 atcaacaccg gccccgagtg cgaacaccgc tttaagatcg cgccctgcca gcatttcaac
     5341 aggaggatgc caggctaaag cataatcagc agagtcatta tctccgcttt tccatgctct
     5401 gactcttgcc tgaggaatag ctttgcgcag tgcctctatc caccattggg tatcgaacgt
     5461 tgggtgataa aagatgatat ccatactgac tcccgaaaag cgttgtgcga aatttattcg
     5521 cacttatcgt tatgatctac aaagaccacc agcataacaa atccgtggtc ggtggccaaa
     5581 aaagcagatt ttgcttatta aaactgcata ttgattgaag tttgaataaa cgcgcgattt
     5641 tttaaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa
     5701 gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt
     5761 gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta taagcgcatg
     5821 ttattgttta acatgcgctt ttttattttc ccttccagac agtatgccat cgggtatgcc
     5881 atctgaaacc ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga
     5941 gcatactggc aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc
     6001 agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat
     6061 ctgatgctga acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca
     6121 tccagacgaa gggtgatgtt tccccggttg tcggggattg gtttaccgct gaacaggtgc
     6181 aacattcttt ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatccc
     6241 caccggtccc atctcaccag attgttgacg ataattttac tgccaaattt acaggggcaa
     6301 ttggttttgt aattccagtt cagccctctg aagacgttga tcacccctcg ctcaaacacc
     6361 tcatccttat tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc
     6421 ggaaaattat cgtactccag tgaccggtac catgtatcac gggcctgcgt atccattaat
     6481 gacagcatcc cggaacgttg catcaggtca cgccagattt cgcgatcaag attccgggta
     6541 atggctttca ttgctgtttc gggtttctcc atcagccagc agccgcagcg aaaatcctgt
     6601 ttcatggccc agtccggggc agttttacct ccgatgcttt ccgtcagcac cgagatttca
     6661 gccagttgtc ggataagtgt gtcgatttgc aacagtgtac tctttcgtac agccagaatg
     6721 tggcgaatgc tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgggag
     6781 attgtgatat ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg
     6841 tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt ctggtgcaga
     6901 agctgcagat gacgcagggt acggacatcc tcccgggcct gttcgataca gggcaacagt
     6961 gctgctgaat ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc gcagtcgata
     7021 ttgtcctgca gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg
     7081 ttgttcctgg ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca
     7141 gatgaatgag actgacgcca taacgccgga tgagtgtgac gaccagacga cggaacgttt
     7201 ttgacagccc caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca
     7261 gatatccgct acctgtaaaa tgaatccatt ctgattcacc gaagtcactg gtctggtgtg
     7321 acagcgagta caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc
     7381 cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat tcatcaacaa
     7441 cacgaaggga gtagccgtta tcgcagacca tgttaatcag ttcagcgagc tcaataccat
     7501 cgacgtcaac gacaatgcgc cccatgttca gtgcctgtac gttaacgctg tcggcttcca
     7561 gtgtcagggc cagtttcatc atttcgcctc cggatactta cccagggtaa tgttatttac
     7621 cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga ggatatcaat
     7681 actgtttatt aactgagagc ggggacaggt gctgaatccc ggctggtcgg tacgcaccag
     7741 cgcgtatttt tccacgagaa aattaactgc atcacacagt gaaatgcctg cctcaatatg
     7801 ctgctcaatc acacgttcat cggcaaacgg tgtgtcattc agtgtgaggc cataatgctg
     7861 gtccagcagt cggatgagca gtgtctgcca gatggtgacg ggagacgggc agcgagatac
     7921 ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac tggtagtgct
     7981 aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc tggtgtcgga taaacagcca
     8041 gataaacgta accacaggag ccgagggtgt cggcttcgca ggtcagccct tttgcattca
     8101 gcgtgacggt atgctgatat cggggattca gttcaccgct ggtgagcatg agttccagtt
     8161 gtttcatcag cagcggaaag gcctggtcca gatggtatgc atctgcgtcg ctgaagcggc
     8221 ctctgatacc ggcgcggtcg gcaaggtaat gcaggcggtt accctcttgc accagacgag
     8281 cgccaaaaca gggcctcact gtgcagggaa gcccccacca ggggcggtcg tgattgtcat
     8341 cgggatgagt tatcccggag agtttgtctg acacgataaa atcctctctg aaaagtggtt
     8401 aagaatggtt taacgatgac aggtcagccg gcgcgctgac cggtttcggt gaagatgtat
     8461 gcattgatga gaatggcctc atccacagcg gcgtctgagg tcatctggtc gtactcgttt
     8521 cccgggtgac ggaacatcag ttcagggtga cactcatcag ccggacatac gggccaaaac
     8581 tgtccttacg gcgttctgca aacacggcaa gaacgccggg aatatcctgc acttcacgac
     8641 cagtatacgc ttcagcactg ccgtgccagc gatacttacc ggtacagaac ggaaaaagac
     8701 gggatgcagg atgctgttgg tgaatacgca tggcttcacc acgggtgatg attctcatga
     8761 tgtgatacct ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg ttgtgacggg
     8821 ttaaagcaga ccatgttcgg caaaggagaa aacctggttg ccaccgacta tcagatggtc
     8881 cggcacccgg atatccacca ggcccagtgc ctgtaccaga cgttcggtga taagccggtc
     8941 tgccttactg ggtgtgactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     9001 attgtggtac agggcgcgtt taatcacttc ccggggatga acttccgtac ggttgatggt
     9061 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     9121 gaactcttcc cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcacgggt
     9181 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggtttgca gggcccgcag
     9241 aatgagactg cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct gctgcattgt
     9301 ccctgcctcc attcagtcaa tgatgcgcat aatggcgctg cattccggat gctgcagggc
     9361 gtaatcccgc agccggtaat agtggaccgt catggcataa cactccgtac gacaggcatg
     9421 atgactgtac gtcatcagac aggcagcaat accggcggct tccgggctca tttcagcgcg
     9481 gttgccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg
     9541 tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc
     9601 ttcgcaaaga cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat
     9661 gaggccaaaa tgctgcggcc agaagctgat gcgctgttca tcagggacgg gagtggcaac
     9721 cagctgaggt gggttattcc ctgatgcagt ggttacggaa acagaaggtg tggtggaatt
     9781 atgcgaaacg gttgtcatga ggttattcct tataaaaagt aaatgaatgg aagaagcccc
     9841 ggggcaggaa cagatgtgag tcaggactgc actttcagtg aaacggcatc agcgcatgct
     9901 ctccagcagc gtttctgcca tcacccacaa tgcgcgattg agcttaatgt cggtatcaat
     9961 gctgtgaatg gcccgggtat ggatacgttt tccttttgca ctgcgaccgg aaatcccgcc
    10021 tttcagcata ttctcctgga tggtctgata agcactccac aggtccttac cgtaatcctc
    10081 ccggcgtcgt ggtgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt caccataacg
    10141 gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg
    10201 catggcatca cgcttctcct ctatccggtc aaaaacgccc accacctcgt aagccccttc
    10261 aatgactctg tccaccacat ttccccggtg tggaacacgc aattctccca gagactgacc
    10321 gcagaccagt ccattggtac aaatggcacg aaaatatccg ggtaacatct ggtagctgga
    10381 tgaaccgtca tgggagttga gcagaataat ttcagggaca tgctgaccgg ttatctgtcc
    10441 ggcccgccgc agacgcagca tgtgttttgt gtattcccgg cggctctggt cgcgcacgcg
    10501 ggtctggcag gcaaagaacg gctgaaagcc ttcctgctgc agattttcca ggacggtgat
    10561 agtgggaatg tacgcatagt gttcactgcg agaggtatgt cggttttccc caaaaatact
    10621 cggtacatgg cgcatcagtt cttcgtgtgt cagcggacga tcacgacgta tctggttcgc
    10681 ataaccaaaa cgactggcta atctcatgat ttgctcctta tgaatgatta agcgatataa
    10741 atgaaaaagc cgcgtctccc ggagaagacg cggcctgatg ggtgaaatga atgaggttta
    10801 ttgtctgaga agcccttcac tggcgaacca cgtattaagc tgtattccgg catcgccagt
    10861 gcaactgacc ttcagcattg cggataaccc gccggaaata tgttcactgg ttgtttttgt
    10921 atcttccccc aaaattaaat aaattcatct taaccatcac ccagataata tatatctgaa
    10981 aatattttca ataacaccag acaggaaaaa caccaccaca ggcggtggcg tttctttggg
    11041 gcgtctttat ttaatccggg ccatcgcgtt tctcagtgcc aaatctgcct cttccgttat
    11101 atccaggctt ttctgcaata ccgcctctga ccgggtcagt gcctgacgtg ctgccagcgg
    11161 tttccggata tgctccatcg ccagcatcgt gtcttcatag ccctgctgac gccaggcgtc
    11221 ggtatgtgcc gatgtaaaac tgagcagacc gccgctgttg ccaccatcac ctgcatattt
    11281 cagccttttc cggggacgga tctcaaggat tgtggtgtcc ggaaaagcct ggcgatccca
    11341 taaagaaccg tcactcagat gcgtcacaat caccatattg catccggcat cgaccagagg
    11401 cgtcacaggg gtatttccct gcatatttcg ccatcctccc ataccaccat caccgaacat
    11461 tgtcccctga acctcacggg gacggaacag caggggcagc gcggctgacg caagcagagc
    11521 ctctttctgc tgtccgcggg gcaggctctg gatatgctga aaaacggcgt ttttcgtggt
    11581 tccgacaccc agttcagcac gaatgcagtc aataatatcc tgcatgcccc cttctgtggg
    11641 gtacagcgac acatacagcg gtagcccgtc tgccagagca tcagtatcaa gataatgatc
    11701 catcagcgct gtcaggggct catctgacaa caacggctga gccatcagat tgtcaaaccc
    11761 gttgattgtc gatatatggc gaagaagcgt cgtaagcagt gctcctgcac gtccggggat
    11821 ctggcagagg ttcattgcct ggaacaattt tttcagcaat gaaaagtaaa ccaatctgtt
    11881 taccgacag
//
LOCUS       DAZGXS010000124        11869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGXS010000124 DAZGXS010000000
VERSION     DAZGXS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            53..655
                     /gene="lpfA2"
                     /locus_tag="ACHKG5_003768"
     CDS             53..655
                     /gene="lpfA2"
                     /locus_tag="ACHKG5_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFP0869779.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            816..1142
                     /locus_tag="ACHKG5_003769"
     CDS             816..1142
                     /locus_tag="ACHKG5_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0869780.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1295..1444
                     /locus_tag="ACHKG5_003770"
     CDS             1295..1444
                     /locus_tag="ACHKG5_003770"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0869781.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1466..4000
                     /locus_tag="ACHKG5_003771"
     CDS             1466..4000
                     /locus_tag="ACHKG5_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0869782.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4012..5082
                     /locus_tag="ACHKG5_003772"
     CDS             4012..5082
                     /locus_tag="ACHKG5_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0869783.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5110..6192
                     /locus_tag="ACHKG5_003773"
     CDS             5110..6192
                     /locus_tag="ACHKG5_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0869784.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6404..7444
                     /gene="pstS"
                     /locus_tag="ACHKG5_003774"
     CDS             6404..7444
                     /gene="pstS"
                     /locus_tag="ACHKG5_003774"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0869785.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7531..8490
                     /gene="pstC"
                     /locus_tag="ACHKG5_003775"
     CDS             7531..8490
                     /gene="pstC"
                     /locus_tag="ACHKG5_003775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0869786.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8490..9380
                     /gene="pstA"
                     /locus_tag="ACHKG5_003776"
     CDS             8490..9380
                     /gene="pstA"
                     /locus_tag="ACHKG5_003776"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0869787.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9563..10336
                     /gene="pstB"
                     /locus_tag="ACHKG5_003777"
     CDS             9563..10336
                     /gene="pstB"
                     /locus_tag="ACHKG5_003777"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0869788.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10351..11076
                     /gene="phoU"
                     /locus_tag="ACHKG5_003778"
     CDS             10351..11076
                     /gene="phoU"
                     /locus_tag="ACHKG5_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0869789.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11501..11635
                     /locus_tag="ACHKG5_003779"
     CDS             11501..11635
                     /locus_tag="ACHKG5_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869790.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQKNFVCLLVMRRSIQ
                     "
     gene            11602..>11869
                     /locus_tag="ACHKG5_003780"
     CDS             11602..>11869
                     /locus_tag="ACHKG5_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFP0869791.1"
                     /translation="MSVGNEEKYSITHKDITNLIAGDRQSFLKLTLWEFILDLFPMYH
                     IKEAKETIVEFVTKTEDKVKRFERIKSLAIPEEKWRFSTTTEFTV"
BASE COUNT         3128 a         2732 c         2927 g         3082 t
ORIGIN      
        1 ttaataaatg gataacacac aggaataata tcaaattaat ggagttatat ttatgaaacc
       61 aaatatgatt gtaggagcat tagcgttaac ttctgtgttt atggcaggtc acctacaggc
      121 ggctgatgga acagtccatt tccgtggtga aattattgac agtacttgcg aagtcactcc
      181 tgaaactaaa gatcaggtcg ttgatttagg caaagtaaac cgtacagcct ttagtggcgt
      241 cgatgatgtg gctgccccga cggctttttc tatcgatctg actcaatgcc cggaaacctt
      301 taagtccgcc gcaattcgtt tcgatggtaa tgaagatgct catggtaatg gcaacctggc
      361 aattggtacc ccgctggata actctaacga tgctgccgct ggtattagcc cgagtgataa
      421 cagtggggat tatactggtg cgggtgccgt tagtgcagcg aaaggcgtag ctattcgttt
      481 atataaccgt gcagataaca ctcaggtcaa gttatatgaa aattctgcat caactccgat
      541 ttctaatggt aatgcatcca tgaagttcat ggctcgttat attgctacgg aaacgactat
      601 tgaccctggt acagctaacg ccgactcgca gtttacagtt gaatatataa aataaatcat
      661 tcttaacata ataccttaaa taaaatggcg ctcgccatta tggttctctt ttacttaaat
      721 gaagggcgtc attattatat tttttatgag atacatttat gcctatgtat ttacttagat
      781 tgttttttgc tgttactttg attacgcaga tgtcgatggc tacggctggt attgttgttg
      841 ggggaactcg cgttattttt tatggtgaca aacctgatac cacaatctct atttttaata
      901 aagaaacgaa ccttccttat ctattgcaga catgggttga tccatttaat aaagaggata
      961 aaagcaaacc gccattttct gtgatacctc ccgtttctcg cattgaacca cagcaagaga
     1021 aaatattacg cattttaaaa acggctggcg agttaccaaa agatcgggag tccgtattct
     1081 ggttgaacgt gaaaaacatc cctccttcaa ttgataaaaa aataccaatg ttcttgagat
     1141 agccataaaa acaagaatta agttattctg gcgaccagcc gcggtaaaga taatccctga
     1201 gcaggctatt aaaaatgtta aatggtcgcg gcagggtaat aatctcatcg tcgaaaatcc
     1261 aaatccgatc catattaatg taatgagtgt gacggtggat gataaagacg tgtcgctaaa
     1321 tatgatccgc ccttttgaaa tattgacatt accaattccg gctggcgtgg caggaaaatc
     1381 gctcgtttgg cgctttatta atgattacgg tgcaattagt caaccactaa agaaaaacct
     1441 ttaagcgccg gataaggata tatcgatggc ctcttcgcat cttttcatta cgcttgcatc
     1501 gggcatatgt ctgctctgtt ccatatctgc ttttgcccgg gatagcttgt tcaaccccag
     1561 attactggaa ctggatcatc ctgcggataa tattgatatt caccagttca accgttcgaa
     1621 taccttacct gcgggaacat acaaagttga tgtgatgatc aacggcatgc tcttcgaacg
     1681 ccaggaagtt aaattcgccc aggataaccc tgatgctgaa ctccacccat gctacgtggc
     1741 gataaaaaac gtgctggcga cctatggtat aaaagttgat gcgataaaat ctctggcgaa
     1801 tgttgatgac aaaacatgcg taaatccagt tccgctgatc gacggggcta cctggttact
     1861 ggacgccagt aaacttgcat tgaatattac tattccgcaa atttatctca acaatgcagt
     1921 taatggttat atcagccctt cccgttggga tcaggggatc aatgccatga tgatgaatta
     1981 tgatttttcg gcatcgcata ccatccggtc aaattatgac gacgacgatg acagttatta
     2041 tctgaatttg cgtaatggta ttaatttagg cgcatggcgt tttcgtaatt acagcaccct
     2101 gaattcttat gacggtaatg tggactacca ttccgtcagt aattacattc agcgcgacat
     2161 catggcatta cgtagccaga ttatgattgg cgatacctgg acggcaagcg atgtatttga
     2221 tagtacacag gtgcgtggcg tgcggctgta taccgatgac gatatgttgc cctccagcca
     2281 gaacggcttt gcgccagtgg tacatgggat tgcgaaaact aacgccacgg tgatcatcaa
     2341 acaaaacggc tacgttattt atcaatcagc cgtaccacag ggcgcatttg ccctcaccga
     2401 cttaaacacg accagtagcg gcggcgatct cgatgtcact atcaaagaag aagatggcag
     2461 cgagcagcac tttattcagc catttacttc actggccatt ctcaagcgtg aaggtcagac
     2521 cgatgtagac cttagcattg gagaagtgcg cgacgaaagc ggctttacgc ctgaggtctt
     2581 gcagttacaa gcaatgcacg gtttcccttt gggaataact ttgtatggcg gaacacaatt
     2641 ggcaaatgat tacgcttctg ccgcgctggg tattggtaaa gatatggggg cgctgggcgc
     2701 gatttctttt gacgtgactc atgcccgctc gcagtttgac tacgacgata atgagagtgg
     2761 tcaatcgtat cgttttctct attccaaacg ttttgaagac accaatacca cctttcgtct
     2821 ggtgggttat cgctactcta tggaggggtt ctacaccctc aatgaatggg tgtcgcgaca
     2881 ggataatgat tctgatttct gggtaacggg caaccgtcgc agccgcttcg aaggcacctg
     2941 gacgcaatct ttcacgccag gctggggcaa tatttattta acattcagtc gacaggaata
     3001 ctggcagacc gatgaggtcg aacgtttatt acagttcggc tataacaaca actggcgaaa
     3061 catctcctgg aacgtttcct ggaactatac ggactcgatc aagcgctcat tgggcaacca
     3121 tcatgatgat aacaatgatg atttcggcaa agaacagatt ttcatgttct caatgtcgat
     3181 accgctatcg tgctggatgg aagacagcta cgtcaactat tcgttaacgc aaaacaacca
     3241 ccatgaaagc acgatgcagg tcggtctgaa cggaacgatg ctggaagggc gtaacctgtc
     3301 ttataacgta caggaatcgt ggatgcactc tcctgatgac tcctacagcg gcaatgccgg
     3361 aatgacctat gacgggactt atggctcggt caatggtagc tattcctgga gccgtgactc
     3421 ccaacatttt gattatggcg ccagaggcgg cgtgctggtg catagtgacg gagtgacctt
     3481 ctcgcaggaa ctgggcgaaa cggtggcatt ggtcaaagcg ccgggcgcag aaggcctgtc
     3541 cattgaaaac gccaccggga tttctaccga ctggcgtggt tataccgtaa aaacgcagct
     3601 tagcccgtat gacgaaaacc gcgtggcatt gaacagcgac tatttctcca aagccaatat
     3661 tgaactggaa aacaccgtca tcaacctggt accaacgcgc ggtgcggtgg tgaaagccga
     3721 atttgtcacc catgtcggtt atcgcgtgct atttaacgtc cgccaggtca acggtaaacc
     3781 aataatgttt ggcgcgatgg caaccgcctc tctcgaaacg ggcacagtca ccgggattgt
     3841 cggtgataac ggcgaactgt atctctccgg gatgcctgaa aaaggcgagt ttttattgag
     3901 ttggggacaa gctgcggatg aaaaatgtaa ggcggcctat cacatcaccc ataaacctga
     3961 tgataccagc ctggttcaaa tggatgcgat ttgtcgctaa aggaagcatg aatgaaaaaa
     4021 atattatcag ggttgattct gctgctttgc tgtccttatg gtttcgccgc taatggtgat
     4081 ggcgcaacgc acatgtcaaa tttatcattt ggtccgctga cggtggcagc ggcgaataac
     4141 cactccggat acaatatttt cgaggcactg agcaacacga ctggaacata cccggtgcgc
     4201 tgtcactgtg atgacacgca tggcggaccg ggccaacaaa cagcattttt tcctatcttc
     4261 tacacgggag atgccgcacc ggggcttgtg cttgagcgca ctcttaatgg gttaaattac
     4321 tatgctctga atgattattt atcggtcggc gtgacgattt ttattattaa taaccaatat
     4381 gcggccattc cttttgaaca cttatccaac caatccacct caccgcaaca tacctgtgga
     4441 gcaggtaata atgggagcac tgtaaatctg gattcagggc gctcggcaaa attatctttc
     4501 tatgttcggc attctattac tggcacggtg acaataccca caacggaagt cgcctggttg
     4561 tacgcgggca tgtccgatca ttttcccaaa acgacccccg tttctaaagt gacaattcgc
     4621 ggacaactaa cggccccgca gaactgtgag ttaacgccaa atcagagcat cgatgtcgat
     4681 tttcaaaaaa ttaatagcgc tgagttctcc tcaacggcgg gttcaattat tgcagaaaga
     4741 aagattaaaa ccgaagtcac agtatcctgt accgggatgg aagacgtaag gtccacggag
     4801 gtggtgagtg cgtcgatgat tgcggcaaac agaagtgccg atgccaccat gatcgtgacg
     4861 agtaatccgg atgtgggaat taagattttt gataagaacg accgtccagt gaatgtggat
     4921 gggggcaact tacccgctga tatgggtgct attagtcgat taggaaaaac cgatggtagc
     4981 gtaacgtttt attcagcgcc cgccagtctg acgggcgcaa aaccagcgcc tgataatgga
     5041 tttaccgcta cagccacgct ggttattgaa tttactaact aatcttgtgt tagtaaaagg
     5101 aataatttca tgaaaaaatg gcattatata ttttgcataa ttctctttca tttagggtta
     5161 ccgtgcgggt atgcggcaaa tgatggcacg tgtgcaacaa gaggcggcac acatacatta
     5221 agccttaatt ttcctctgac aacggtcagt gcagcaaaca atgtgcctgg aaatacatta
     5281 atagatattg ctaatgcaac atcttctgaa aattatagcg ttctgtgtaa ctgtgattca
     5341 aaacatagca atggcgctta tcacgaaata tattataccg cagaccctgc tcccggtatg
     5401 gtttatagca ccaccgcaag tggtcttgct ttttactatc ttaacgaata tgtcgatgtg
     5461 ggaacaaaaa tatctgtgct aaatgcgggg tatacggcag ttccttttga acatgtttcc
     5521 aaccaggcaa ctacaacaga tcacacttgt cagggaaaca aaactacagc ggttggcgtg
     5581 agcctgaaaa ctggagcaga tgcgaagatt tcatttcgta ttaaacgttc aataaatgga
     5641 acggtagtaa tacctatcac cgatattgca ttgctgtatg ccaacatatc cagcaccacg
     5701 acccgtggtg aggcgattgc aaaagttcga atttcaggca gtttgaccgc accacagtct
     5761 tgtcagataa atgcaggaca ggtgatttat tttgattttg atactattcc tgcgtccgaa
     5821 ttttcatcta ccgccgggca agccattact tcacgaaaaa tcactaaaac agtgagtatt
     5881 gagtgtacgg ggatggggta tgagcgtacg cagaaagtcg atgcttcttt tacggggacg
     5941 aaccgaagca gtgacgatac gatggtggcg acagacaatg ctgatgtcgg gatcaaaatt
     6001 tacaataaat cgaatgctga agttagcgtc aacaacggca agttacccgc agacatgggc
     6061 aacacgacca tttttggtcg taaaaatggt tcggtaactt tttcggcagc acctgccagc
     6121 tttaccggtg cccggcctca gcccggcgtt tttaacgcta ccgcgacctt aaccattgaa
     6181 tttgtaaact aaccttttgc ggctgtttta tgacagccgt tcatatttcc tctcctttca
     6241 tgaaaaaatg ctgatatttt tccccttcaa tatcaataag taataacttt cctttgggtg
     6301 tcatcaaact gtcacattcc ttacatataa ctgtcacctg tttgtcctat tttgcttctc
     6361 gtagccaaca aacaatgctt tatgaatcct cccaggagac attatgaaag ttatgcgtac
     6421 caccgtcgca actgttgtcg ccgcgacctt atcgatgagt gctttctctg tgtttgcaga
     6481 agcaagcctg acaggtgcag gtgcaacctt ccctgcgccg gtgtatgcca aatgggctga
     6541 cacttaccag aaagaaaccg gtaataaagt taattaccag ggtatcggtt cttccggtgg
     6601 cgtaaaacag attatcgcca ataccgttga ttttggtgct tctgacgcgc cgctgtctga
     6661 cgaaaaactg gctcaggaag gtctgttcca gttcccgacc gtgattggcg gcgtggtgct
     6721 ggcggttaac attccaggtc tgaagtctgg tgagctggtg ctggatggta aaaccctcgg
     6781 cgatatctac ctgggcaaaa tcaagaagtg ggatgatgaa gccatcgcca aattgaatcc
     6841 gggtctgaaa ctgccttcac agaacattgc tgtagtgcgc cgcgcagatg gctccgggac
     6901 ttccttcgtc ttcaccagct acctggcgaa agtgaatgaa gagtggaaaa acaacgttgg
     6961 tactggctct accgtaaaat ggccgatcgg tctgggtggt aaaggtaacg acggtatcgc
     7021 tgcgttcgtt cagcgtctgc cgggtgcaat tggttacgtt gaatatgctt atgcgaagca
     7081 aaacaacctg gcgtacacca aactgatctc cgctgatggt aaacctgttt tgccgaccga
     7141 agaaaacttc gctaatgcag caaaaggtgc agactggagc aaaaccttcg ctcaggatct
     7201 gaccaaccag aaaggcgaag atgcgtggcc tattacctct accacgttca ttctgatcca
     7261 caaagatcag aagaaaccag aacaaggcac agaagtgctg aaattcttcg actgggcgta
     7321 caaaaccggg gctaaacagg cgaacgacct ggattacgcc agcctgccgg atagcgtagt
     7381 tgaacaggtt cgcgctgcgt ggaagaccaa tattaaagac agtagcggca agccgctgta
     7441 ctaataaaac tccaggccgg ataaggcatt aaacggctta tccggcatta caaaatgact
     7501 ttgtaaacgc gtttaactga agagtaactt atggctgcaa ccaagcctgc ttttaaccca
     7561 ccgggtaaaa agggcgacat aattttcagc gtgctggtaa aactggcggc gctgattgtg
     7621 ctattgatgt tgggtggcat tattgtctct ctgatcatct cctcatggcc gagcattcag
     7681 aaatttggtc tggctttcct gtggaccaaa gagtgggatg cgccgaacga tatctacggg
     7741 gcgctggtgc cgatctacgg tacgttggtg acttcgttta tcgcgctgct gatcgccgtc
     7801 ccggtgagtt tcggtatcgc cctgttcctg actgagcttg cgcctggctg gctgaaacgc
     7861 ccgctgggta tcgccattga gctgctggca gccattccaa gtatcgttta cggcatgtgg
     7921 ggcctgttta tctttgcgcc gctgttcgcc gtttacttcc aggagccggt cggcaatatc
     7981 atgtcgaata tcccgattgt cggcgcgctg ttctctggcc cggcatttgg tatcggtatc
     8041 ctcgcggcag gcgtgatcct cgccatcatg attattccgt acattgcggc ggtaatgcgc
     8101 gatgtgttcg aacaaacccc ggtgatgatg aaagagtcgg cctacggtat tggctgcacc
     8161 acctgggagg ttatctggcg tatcgttctt ccgttcacca aaaatggtgt tatcggcggc
     8221 attatgctgg ggctgggccg cgcgcttggt gaaaccatgg cggtgacctt tattatcggt
     8281 aacacctacc agctcgacag cgcctcgctg tacatgccgg gcaacagtat cacctctgcg
     8341 ctggcgaacg aatttgccga agcggaatcc ggtctgcacg ttgccgcact gatggaactg
     8401 ggcctgatcc tgtttgtgat taccttcatc gtcctcgccg catcgaagtt tatgattatg
     8461 cgcctggcta agaatgaggg ggctcgctaa tggctatggt tgaaatgcaa accactgcgg
     8521 cgctggcgga atcccgccgc aaaatgcagg cgcgtcgccg cctcaaaaac cgtattgcac
     8581 tgacgctctc aatggcgacg atggccttcg gtctgttctg gctgatctgg attttaatgt
     8641 ccaccatcac ccgcggtatc gacggtatgt cgctggcgct gttcactgaa atgacgccgc
     8701 cgcccaatac ggaaggtggt ggtctggcga acgctctggc aggtagcggg ctgttaattt
     8761 tgtgggccac ggtattcggt acgccgctgg gcattatggc ggggatttat ctggctgaat
     8821 atggtcgtaa atcctggctg gcagaagtga ttcgcttcat taacgacatt ctgctctctg
     8881 cgccgtcgat tgtggttggt ctgtttgttt acaccatcgt ggtggcgcag atggagcact
     8941 tctccggttg ggcgggtgtg attgcgctgg cgttgttgca ggtgccgatt gttatccgca
     9001 ccaccgagaa catgctgaaa ctggtgccgg acagcctgcg tgaagcggct tatgcgctgg
     9061 gtacaccgaa gtggaagatg atctccgcga ttacgctgaa agcgtcggtg tccgggatca
     9121 tgaccggtat cctgctggcg attgcccgta ttgctggtga aaccgcgccg ctgctgttta
     9181 ccgcgctctc caaccagttc tggagcacgg acatgatgca gccgatcgcc aacctgccgg
     9241 tgacgatctt taagtttgcg atgagcccgt ttgcggaatg gcagcaactg gcctgggccg
     9301 gggtattgat cattaccctg tgcgtactgc tgctgaacat tctggcgcgc gttgttttcg
     9361 cgaagaataa acacggttga tattgctgac acggttttcc cctcacccta accctctccc
     9421 cagaggggcg aggggaccga ccgagcgcct ttttgactct gtacacggtt aacactttgc
     9481 cggatgcggc gtgaacgcct gatccggcct acggtaagcc tgattcgcga agtgcatcag
     9541 gcacgatgag gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt
     9601 tcgtaatttg aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat
     9661 cgccaaaaac caggtaacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct
     9721 gcgtaccttc aacaaaatgt ttgaactgta cccggaacag cgtgcggaag gtgaaattct
     9781 gctcgatggc gacaacatcc tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt
     9841 gggcatggtg ttccagaaac cgacgccgtt cccgatgtcc atctacgaca acatcgcctt
     9901 tggcgttcgt ctgtttgaga agctctctcg tgccgacatg gacgagcgcg tgcagtgggc
     9961 attgaccaaa gccgcattgt ggaacgaaac caaagataaa ttgcatcaga gcggttactc
    10021 tctctctggt ggtcagcaac agcgtctgtg cattgcgcgt ggtatcgcca ttcgcccgga
    10081 agtgctgctg ctcgacgaac catgttcggc gctcgacccc atctccaccg ggcgtattga
    10141 agagctgatc accgaactga agcaggatta caccgtggtg atcgtcaccc acaacatgca
    10201 gcaggctgcg cgttgttccg accacacggc gtttatgtac ctgggcgaat tgattgagtt
    10261 cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac
    10321 cggtcgttac ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt
    10381 tccggccagt tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggcggc
    10441 atggtggagc agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg
    10501 gcgaagcgcg tgatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa
    10561 gcctgcgtgc gcattattgc caaacgccag ccgacggcga gcgacctgcg attggttatg
    10621 gtgatcagta aaaccattgc cgaactggag cgtattggcg acgtggcgga caaaatctgc
    10681 cgtactgcgc tggagaaatt ctcccagcag catcagccgt tgctggtaag tttggagtcg
    10741 ctgggccgtc atactatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac
    10801 attgacgaag cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga gtacgaaggt
    10861 attgttcgtc aactgatgac ctacatgatg gaagattcgc gcaccattcc gagtgtactt
    10921 accgcgctgt tctgtgcgcg ttctatcgaa cgtattggcg accgctgcca gaatatttgt
    10981 gagtttatct tctactacgt gaagggtcag gatttccgtc atgtaggcgg tgatgagctg
    11041 gataaactgc tggcgggaaa agaccccaaa gagtagtgct aactgtaggc cagataaagc
    11101 tttttgccgc atctggcaat cagcccggtc atacatagtt accgggctga tacactttct
    11161 cactgaaata ttgccacaca aactcacctc gaaactctcc ccagtacatt gaatatacat
    11221 tatttcattt gaattatttt acttttaatg tagtgcctcg ctataaaaac atatttgtta
    11281 ttcttttcat ttatatcgct acctgcacag agaatggaaa agaaaacaaa aaacagggta
    11341 aaagccctat atcgagacat aaaaaatgag cgatttttta cagtattcat aggtggttta
    11401 gtcataatcg tagcaaacca acacccaggt atgcttttta agacaaatcg gcaatgtatt
    11461 atgctcgggc gaataaatat tgtgttcagg agaaatgatt atggctatat catcatatag
    11521 tacagttaca gataaatttg ctaatctgac taaaatgcca gatgaaaata atggattgca
    11581 aaaaaatcag aaaaattttg tttgtctgtt ggtaatgagg agaagtattc aataacccat
    11641 aaagatataa caaaccttat agctggtgat agacaatctt ttcttaaact aactctttgg
    11701 gaatttatac tcgatctctt tcccatgtat catattaagg aggctaaaga aacaattgta
    11761 gaatttgtaa cgaaaactga ggacaaagtt aagagatttg agagaattaa atctctcgct
    11821 attccagaag aaaaatggcg ctttagtacg acgacagaat ttacagtag
//
LOCUS       DAZGXS010000125        11818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGXS010000125 DAZGXS010000000
VERSION     DAZGXS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..476
                     /locus_tag="ACHKG5_003781"
     CDS             <1..476
                     /locus_tag="ACHKG5_003781"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0869792.1"
                     /translation="GYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTV
                     WCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQ
                     LRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQSAQQTQLHLADALTDAAVN"
     gene            697..1038
                     /gene="symE"
                     /locus_tag="ACHKG5_003782"
     CDS             697..1038
                     /gene="symE"
                     /locus_tag="ACHKG5_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP0869793.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(1085..2248)
                     /locus_tag="ACHKG5_003783"
     CDS             complement(1085..2248)
                     /locus_tag="ACHKG5_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP0869794.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2296..3177)
                     /locus_tag="ACHKG5_003784"
     CDS             complement(2296..3177)
                     /locus_tag="ACHKG5_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP0869795.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(3274..3438)
                     /locus_tag="ACHKG5_003785"
     CDS             complement(3274..3438)
                     /locus_tag="ACHKG5_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0869796.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3615..5027
                     /locus_tag="ACHKG5_003786"
     CDS             3615..5027
                     /locus_tag="ACHKG5_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0869797.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(5270..6227)
                     /locus_tag="ACHKG5_003787"
                     /pseudo
     CDS             complement(5270..6227)
                     /locus_tag="ACHKG5_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            6694..7926
                     /gene="mdtM"
                     /locus_tag="ACHKG5_003788"
     CDS             6694..7926
                     /gene="mdtM"
                     /locus_tag="ACHKG5_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP0869798.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            7967..9238
                     /locus_tag="ACHKG5_003789"
     CDS             7967..9238
                     /locus_tag="ACHKG5_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0869799.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9354..10505
                     /locus_tag="ACHKG5_003790"
     CDS             9354..10505
                     /locus_tag="ACHKG5_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0869800.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAEESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10515..11282
                     /gene="yjiL"
                     /locus_tag="ACHKG5_003791"
     CDS             10515..11282
                     /gene="yjiL"
                     /locus_tag="ACHKG5_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0869801.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            11279..11545
                     /locus_tag="ACHKG5_003792"
     CDS             11279..11545
                     /locus_tag="ACHKG5_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0869802.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         2876 a         2951 c         2897 g         3094 t
ORIGIN      
        1 gcggatatct tgatcttacg gaaattaaac agattgaaat ccctattgag gaaaaagaaa
       61 aatatcaagt agtcaaggga gatttattga taacagaagg cggcgactgg gatacagtcg
      121 ggagaactac agtatggtgt catgactggt atatagcaaa tcaaaaccat gtattcaaag
      181 gacgaaatat agggcaagat gttgatccat attggttaga aacatatatg aatagcccat
      241 tctcaagaca atattttgct aacgcaagta agcaaaccac taatttagct tctattaata
      301 aaacccagct cagaggttgt cctgttgcta ttcctcctag ctcagaagca aaaaaaataa
      361 tgagtaaact acatattttt tataaactat gtgaagaatt aaagaatcat atccaatccg
      421 cccagcaaac ccaactacac cttgcagatg cactcactga cgcggcggta aactaattta
      481 cctgccctcg aaaaaccaat aaagcggcta aatagccgct ttattcacat caccaaaatt
      541 tatatttcac gctaattttc atcttatttt cattaatttt tagctggact ttccccatat
      601 ttactgatga tatatccagg tatttagcgc ggtgcggatg tgcgccaaca cacccgcacc
      661 gctaatcaca atcactattt atagggaata gcagttatga ctgatacgca ttctattgca
      721 caaccgttcg aagcagaagt ctccccggca aataaccgtc aattaaccgt gagttatgcg
      781 agtcgttacc cggattacag ccgtattccc gccatcaccc tgaaaggtca gtggctggaa
      841 gccgccggtt ttgccaccgg cacggtggta gatgtcaaag tgatggaagg ctgtattgtc
      901 ctcaccgccc aaccacccgc cgccgccgag agcgaactga tgcagtcgct gcgccaggtg
      961 tgcaagctgt cggcgcgtaa acaaaggcag gtgcaggagt ttattggggt gattgcgggt
     1021 aaacagaaag ttgcctgata cattgacccg gtagcgccag gactaccggg catttttcgc
     1081 tgcgttatac caacgcgctc tccatctgtt tttcaaatgc ctgacgaatt aaccccataa
     1141 tgtaaggaag ttgtgcgagg tcactgaaac caatttccac atcaccattg ccccatcgcc
     1201 caacatttgt cacatctttg gcaatacctt tcggatcgac cagttcatga aactgcatat
     1261 taagcgacaa tcgcagtcgg cttttttgcg gcacaacatc aacaaaattc gtttccgctt
     1321 taaacgcaat atagagcttc aatacttcct gcgtgatccc tgcgtccagg cgcataattt
     1381 catcgcgaag atgatcgaat aacacccggc tatgcgaacc atcagcaaga aaaggataat
     1441 cactcaggct gtaactggtt ttgttctcag gctgtccccg ataaacagct aaaacctctt
     1501 caggaagaga aggcgcttgc cagacctgaa ccgcaagctc cgccagacga tcggctcggg
     1561 cgtgaatggc agcttcatcc catttttcgc actgtccaag accgatattc aaatagagcg
     1621 ggctatgctt aaagccgtct tcaatatcgc gtttttctgc gaaaggtctg tcactgtaac
     1681 gggagttata gcgcgttaaa gtgagattcc ccaacgtatg caacagttct ttatgaatac
     1741 gctgccagtc acttcccagc tcttcgcgcc atttagccga caaattttcg ttctgcggca
     1801 taatatgttc aatcgtaaac tcttccacgc gctctctgcg tttatcgttt tccagtcgtc
     1861 gtaaccagta gctacgacta cggaagttat agagatcgcg aacttttaat tcccgtttaa
     1921 attcatcatc gttggggaaa cgacgatatg aaggtagatt caaaaaatgt acctgaatac
     1981 tttccagata tttttcttta ttaatgacct tataaaaagt agcaaacgtc ttgttaagag
     2041 aatttgtcgg aatcgcacat actgcacggc ggaaaacata agattcaatt aaacgaatta
     2101 tgctcaggaa atcttcgtga gacaaatcgt catttttata gtcatgataa agcgccagta
     2161 agaaaggata cgccacatca acctttaact cgcgcaaatc ctgaaaagcc gtagcaagcg
     2221 atttgtcact ttcttttccc aatgccattg cacagtaata ctccgcgtag atatgaatat
     2281 cttcaaccag ccgatctacg cctttttcag caacactctg gctgcgggca tggagtttaa
     2341 atgcctcata gacttcatct gtccgaggga tctccccgtt tttaccgtca gataatgacg
     2401 cataaaactg tcaaaatatt cgctgtaacc ttgctgacca caagcgactt ccattggacg
     2461 ccagtgatct tcatacaacc gggtttgatg ctctggttcg aggcccatca gaataaaatt
     2521 gcgcaccaga tcggcctgac ttaacgcctt accggtggag ttcatacttt caaaaatcag
     2581 ttgcggatta tcctgaccac gattaagcgc cacatcgaca attaataact ttgctaaccc
     2641 acgacataag gggatcaagt catcaccgag tttggcaatt tgttcatcaa agaaagtaaa
     2701 gttttccatt attcgatggg aatagttttc tggcattggt ctttgtttta ttaacgccag
     2761 taaactgtct ttatcagtct cggtcagcag taatttaaaa cctttctcgc cggactcata
     2821 aggattgagc aaataataat tacgcaattt cattgccgaa aagccatcaa aaacttcgtc
     2881 ttcgccaaga tggcgcgata aagcctcaat cagcaacatt gcggtcgtca gccgctgctg
     2941 accatcaatg accagcaacg gagaaatacc agaaacctga tacaatccct gctctatata
     3001 aacaaccgaa ccgataaaat gcgctgatat atcgtcacgc tttcctgcac gaatgatgtc
     3061 gtcccaaagt tgccgacatt gttgttctgt ccatgaataa gtccgttgat aaatgggaat
     3121 aacaaactgc tgcgaacgtt ttaaaaaatc gagtaggcga gcttctgtag ctttcattct
     3181 gacgtctttc cgtgaaacga tgatttcgca ataataacca cttccttatg gaggttatag
     3241 agaaatcctg tttatccgcg acgaacatac ccctcactcc acatcctccc tgcgtaaccc
     3301 aatatccctc aaccgttcat cactcatctg ctgtaacacc cgtcgggcct gcatttgccg
     3361 ccgccaacgc ctcactgcct gccagagttg cacaagtctg ataaacggcg ttttggcttt
     3421 gttttcgtga aattccatct gctgcctcct catatttcag aggcttcatc atccgtcttc
     3481 tggcagttca caatacagat gcaaatatta gttttgttta acatacagac aggttaaaac
     3541 agcatctgca tccggttttt tagcctcatc tgtaccggtt tttctttctg tatggtccaa
     3601 ataaggaata cagcatgacg cgttatcaac atctggcgac tctacttgcc gagcggattg
     3661 agcaagggct gtatcgtcac ggggagaaat tgccgtcggt gcgcagctta agtcaggagc
     3721 acggcgtcag catcagcacc gtgcagcagg cgtaccagac gctggagacg atgaagctca
     3781 tcactccgca gccgcgttcg ggttattttg tcgcacaacg taaagcccag ccgccagtgc
     3841 cgccgatgac gcgtccggtg cagcgcccag tggaaattac ccagtgggat ctggtgctgg
     3901 atatgctggt ggcgcatagc gacagttcca ttgttccgtt aagcaaaagc acgccggatg
     3961 tcgaaacgcc cagcctgaaa ccgctgtggc gggagctaag ccgggtggtg cagcataatc
     4021 tgcaaaccgt tctcggttat gacttgctag ccggtcagcg ggtattgcgc gagcaggttg
     4081 cccgcctgat gctcgacagc ggctcggtgg tcactgccga tgacatcatc atcaccagcg
     4141 gctgccataa ctcgatgtcg ctagcgttaa tggcggtgtg taaaccgggc gatattgtcg
     4201 cggtcgaatc cccctgttat tacggttcaa tgcagatgct gcgcggcatg ggcgtgaaag
     4261 tgattgaaat cccaaccgat ccagaaacag gcatcagcgt tgaagcgctg gaactggcgc
     4321 tggaacagtg gccgattaaa ggcatcattc tggtgccaaa ctgtaataat ccgctgggat
     4381 ttattatgcc ggacgcgcgc aaacgggccg ttctctctct cgctcagcgt catgatattg
     4441 tgatttttga agatgatgtc tacggcgaac tggcaacgga gtatccgcgc ccgcggacca
     4501 ttcattcctg ggatatcgac gggcgagtgc tgttgtgcag ctcgttcagt aaaagtattg
     4561 ctccaggcct gcgcgtgggt tgggtcgcac cggggcgtta tcacgataaa ctgatgcata
     4621 tgaaatacgc catcagcagc tttaatgtgc cgtccacgca aatggcggcg gcaacgtttg
     4681 tgctggaagg tcactatcat cgccatatcc ggcggatgcg gcagatctat cagcgcaatt
     4741 tggcgcttta tacctgctgg atacgggaat attttccctg cgaaatctgt attacgcgcc
     4801 cgaaaggcgg atttttactg tggatagaat tgcctgaaca ggtcgatatg gtctgcgtca
     4861 cgcggcagct gtgccgcatg aaaatccagg tggcggcagg ctcgattttc tcagcttccg
     4921 gcaaataccg taactgcctg cgcatcaact gtgctttgcc actcagcgaa acttatcgcg
     4981 aagcgctgaa gcaaattggc gaggccgtgt atcgggcaat ggaataaatt agaattagtg
     5041 gcgaagcacc tgatttattc tcacgaatcg cctgtgactc gttttatctt tgcaggatca
     5101 tagccacgtt ccggcatggc ttaggtaaag tgcgctttgt tcatgccgga tgccatgtga
     5161 acgccttacc aggtctacaa aatctttaaa gttcaatata ttgaatgggc tttgtagacc
     5221 tgataagcgt agcacatcag gctgctttgc gtttgtcatc cgtctctggt tagctcttga
     5281 gccatgaata gcgctcacgc tcaggcaagg gctgccttaa tgcctgcatt tgctctgccg
     5341 aaagtccggt aatcttctgt atccattcag gatctgcccc attctgcaac aacaatcgaa
     5401 gaatgcgctg ttccccttct tcccttccca acagccatcc atcattatga attcgctctg
     5461 caatcgtcat tatgaattcg ctctgcaatc gtcattattc gctctctgtg ttgaggcatt
     5521 cgactggtta gttcactgat aaactcatta aaacgctctt catcgccagt cagtaaaatg
     5581 taatttaaca gcgcagttat ctggctgtca ttagcacact ctgtaaccag taatactacc
     5641 agttgatcga taagccccat cagatcgcgc tggcgaatat gcttttggat caactccaac
     5701 agggcgactc tgcggtgctg cacaatctcg tcgtccggca cgacagtgat atccaccagc
     5761 gggaacgcgg cgttataaag cttccgtgca gtagtcgggt cggcaaattc gtccaaccag
     5821 cacaaagacc agggataagg actacggcta ccgtgataaa acagcatcgg gatgaccagc
     5881 ggtagtggcc ggcgtttatc atgctctata tggcgctgca tcactgccat ggaatagcgc
     5941 atcaggcgaa aggccatatg aatatcctca cggctctgat gttcaatcac cacatagata
     6001 tagccatcgc cttcccgggt ctttaccgac cacagaatat cggagtgtag cgcccgcaat
     6061 ttttcatcga caaagctggc ggattccagt tttaagctgt cgagatcgca cagttcacgt
     6121 aaatctttgg gtaagtggat ctccataaaa tcccgcgcgg tgtcagggtg catgagaaaa
     6181 gatttaaata atgcgtcatg cggcgtgctg gtcgtgaagt ttgtcatggc gatccgtcac
     6241 ctgttgataa gatggcccga ctttagcgac taacaaaacc attctcaccc ggcaatttct
     6301 cgtttcccga ggcgctttcc aggctatttt tgttcgtctg caaacgatac atttttccgg
     6361 aaactgactc gcgttttaca tgtcctgtac ccggcagata ttcccccttc caccgcgctc
     6421 ttttgattaa tcgcaaattt taattacact taaggtgtat attttctatg caacccatca
     6481 attcaagagg tgtaatgtgc tgatgactat ttgtaatcgt tatacattct gacccgaagt
     6541 cagaaggtat ttctctgtct gtgtgttcac aggcagtgtg gttgattaca tgaaattcag
     6601 tacatttgtc cttctcgcct acctttcgtc attaccgacg gtattgaatt tcgttttccc
     6661 cgttggggtt ctccggacaa ggagttgttt gttatgccac gtttttttgc ccgccatgcc
     6721 gccacgctgt ttttcccgat ggcgttgatt ttgtatgact ttgccgccta tctttcgacg
     6781 gacctgatcc agcctgggat cattaatgtg gtccgtgatt ttaatgccga tgtcagtctg
     6841 gctccggctg ccgtcagtct ctatctcgct ggaggcatgg cgttgcagtg gctgctgggg
     6901 ccgctttccg acaggattgg ccgcaagcca gtgctgatta ccggggcgct aatatttacc
     6961 cttgcctgcg ctgcgacaat gttcacaacg tctatgacac agtttcttat cgcgcgtgca
     7021 attcagggca ccagtatctg ttttattgcc accgttggtt atgtcacggt gcaggaggcg
     7081 ttcggccaga caaaagggat caagttgatg gcgattatca cctccatcgt actgattgcg
     7141 ccgattatcg gcccgctttc cggcgcagct ctgatgcact ttgtgcactg gaaagtcctt
     7201 tttgccatca ttgcggttat gggttttatc tcatttgttg gcttactgct ggcgatgcca
     7261 gagacggtga agcgcggcgc ggttccattt agcgccaaaa gcgtcttgcg cgattttcgt
     7321 aatgtctttt gcaatcggct gttcctcttt ggcgcagcaa ccatctcttt aagctatatc
     7381 cccatgatga gttgggtggc tgtctcgccg gtgatcctga ttgacgcagg cggcttaacg
     7441 acctcgcagt tcgcctggac acaggttccg gtgttcggcg cggtgattgt cgcgaatgcc
     7501 atcgtggcgc gtttcgttaa agatccgacc gaaccgcgct ttatctggcg tgccgtaccc
     7561 attcaactgg tcggcctcgc gctgttgatt atcggcaatc tgctgtcacc gcacgtctgg
     7621 ctgtggtcgg tgctgggcac cagtctgtat gctttcggga ttggtttgat cttcccgacc
     7681 ttattccgct ttacgctgtt ttccaataac ttaccgaaag ggaccgtctc cgcatcgctg
     7741 aatatggtga tcctgatggt gatgtctgtc tcggtcgaaa ttggccgctg gctgtggttt
     7801 aacggcggtc gcttgccgtt tcatctgtta gccgttgtgg cgggcgttat cgtcgttttc
     7861 accctggccg gattgcttaa tcgcgtgcgc caacatcagg ctgccgagct ggcagaggag
     7921 cagtgatttt tgcgcgatcc tgccgtcagg ctctattctt aacgttatga ataaactcat
     7981 tgaactcaga cgcgccaaaa tgttggcgct ctctttactg cttatcgccg ctgctacctt
     8041 tgtcgttacg ctgtttttgc cgcccaattt ttgggtgagc ggcgtgaagg cgattgctga
     8101 agcggcgatg gtcggcgcgc tggcggactg gtttgcggtg gtggcgctgt ttcgccgcgt
     8161 gccgattccg atcatttctc gtcatacggc gattatcccg cgtaataaag accggattgg
     8221 cgaaaatctc ggtcagttcg tgcagaaaaa atttctcgat acccaatcgc tggtggcatt
     8281 gattcgacgt cacgaaccgg cgttgctgat tggcaactgg tttagccagc cagaaaacgc
     8341 ccgccgcgtt ggtcagcatc tgttgcagat catgagtggt tttcttgaac tgaccgatga
     8401 tgcacgtatt cagcgcctgc ttaagcgcgc ggtccatcgg gcgattgata aggtcgatct
     8461 ttccggcacc agtgcgttga tgctggagag tatgaccaaa aacgatcgtc atcaggtgct
     8521 gctggatacg ctgatcgcac agttgatcgc ccttctccag cgcgataaat cgcgcaagtt
     8581 tatcgcccag cagattgttc gctggctgga gagtgagcat ccactgaaag ccaaaattct
     8641 ccccaccgaa tggctgggcg aacatagcgc ggagttggtt tctgacgcgg tgaattcttt
     8701 gcttgatgat atcagccgcg atcgtgcgca tcagatccgc catgcatttg atcgcgccac
     8761 ttttgccctg atcgacaagt tgaaaaacga tcctgaaatg acagcgcgag ctgatgccgt
     8821 aaaaagctat ctgaaagagg atgaagcctt tctcagtgaa ttgtgggggg atttacggga
     8881 atggctgaaa gcggatatca acagtgaaga ttctcgtgtg aaagaacgta tcgcgcgggc
     8941 aggtcaatgg tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg
     9001 tcacctggaa caagccgcgc accgcgtcgc gcctgagttt tctgcattcc tgacgcgcca
     9061 tatcagcgat acggtaaaaa gctgggatgc ccgggatatg tcgcggcaaa tagagttaaa
     9121 tatcggcaaa gatctgcagt ttatccgtgt caacggtacg ctggttggcg gttgtattgg
     9181 gctaatttta tatttgctgt cacagctccc ggccttgttc cccctcggca atttttagaa
     9241 atccatgaaa aactaagtgg ctaaccgcga gagagatcaa ataaaccata ctaaaagaat
     9301 ggtaataatg tggcctcttt tcattatctc ccgtggtacg gggaaggaaa atcatgtcac
     9361 ttgtcaccga tctacccgcc attttcgatc agttctctga agctcgccag aaaggctttc
     9421 tcaccgtcat ggatctcaag gtgcgcggca ttccgctggt tggcacttac tgcaccttta
     9481 tgccgcaaga gatcccgatg gcagccggtg cggttgtggt ttcgctctgt tccacctctg
     9541 atgaaaccat tgaagaagcg gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa
     9601 gtagctacgg cttcggcaaa accgataaat gcccctactt ctacttttcg gatctggtgg
     9661 tcggtgaaac cacctgcgac ggcaaaaaga aaatgtatga atacatggcg gagtttaagc
     9721 ccgttcatgt gatgcagctg ccaaacagcg ttaaagacga tgcctcgcgt gcgttatgga
     9781 aagccgagat gctgcgctta caaaaagcgg tggaagaacg ttttgggcac gaaattagcg
     9841 aagatgctct gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca ctggccaatt
     9901 tttatcatct tgggcagttc aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg
     9961 tttacggcgc aaccttccgg ttcgataaag aggcgttgat caatgaactg gacgcgatga
    10021 ccgcccgcat tcgtcagcag tgggaagaag gccagcgact ggacccgcgt ccgcgcattt
    10081 taatcaccgg ctgcccgatt ggcggcgcag cagagaaagt ggtgcgcgcg attgaagaga
    10141 atggcggctg ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg
    10201 tggcagaaac gggcgatgtc tacgacgcgc tggcggataa atatctggcg attggctgct
    10261 cctgtgtttc gccgaacgat cagcgcctga aaatgctcag ccagatggtg gaagaatatc
    10321 aggtcgatgg cgtagttgat gtgattttgc aggcgtgcca tacctacgcg gaggaatcgc
    10381 tggcaattaa acgtcatgtg cgtcagcagc acaacattcc ttatatcgct attgaaacag
    10441 actactccac ctcggatgtt gggcagctca gtacccgtgt cgcggccttt attgagatgc
    10501 tgtaaggagt agcagtgaca tattcgattg gcattgattc tggttctacc gccaccaaag
    10561 ggatcttact cgcagacggc gtgattacgc gccgtttcct cgttccaaca ccctttcgac
    10621 cggcaacagc aattactgaa gcctgggaaa ctctgcgcga agggttagag acaacgccgt
    10681 ttctgacgct caccggctac gggcggcaac tggtggattt tgccgataaa caagtgacgg
    10741 aaatctcctg tcacgggctg ggcgcacggt ttcttgcgcc agcaacgcgc gcggtaatcg
    10801 acatcggtgg tcaggacagc aaagtgattc agcttgatga tgacggtaac ctgtgcgatt
    10861 tcctgatgaa tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg atctcgcgca
    10921 cgcttggcac cagcgtcgag caactcgact gcattaccga aaatgtcacg ccgcacgcca
    10981 tcacgagtat gtgcacagtg tttgctgaat cagaagtgat cagcctgcgc tcagcgggcg
    11041 tcgcgccaga agcgattctc gccggagtga ttaacgcgat ggcgcggcgg agtgccaatt
    11101 tcattgctcg tctctcctgt gaagcgccga ttctgtttac tggtggcgtt agtcattgcc
    11161 agacgtttgc ccggatgctg gaatctcacc tgcgaatgcc ggtaaatacc catcctgatg
    11221 cgcaatttgc tggcacaatt ggcgcggcgg taattggtca acgagtgagg acacgccgat
    11281 gaaagagttt ttatttttat ttcactccac ggtcggcgtc atacaaaccc gcaaggcgtt
    11341 gcaggcagcg ggcatgactt ttcgcgtcag cgatattccc cgtgatttac gcggcggctg
    11401 cgggttatgc atttggctga cctgtccccc cggcgaggaa atacaatggg tgatccctgg
    11461 gcaaaccgaa gcgatatatt gccagcaggg cagcgactgg cagtgcgtgg tgcattacga
    11521 tgcagaagcg tcaaccaggc aataggatct ttaaatatac caaccggata attggctgcg
    11581 cataatcctg catgaaggaa gttgtagtaa atatctcgcc tgccgtgaaa tatttcccat
    11641 ccacaaaatg agcgattttt gatagcacct tcctattgcg cgtctaaaat acaatataca
    11701 ttgaattata cttaaaccca taatcatgca tagagaaata aaatgattac cgggtgttgt
    11761 aatgttaaaa gataagtttc tgtgcaaagc ttatctttcc ccaccccctg attatttt
//
LOCUS       DAZGXS010000126        11786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGXS010000126 DAZGXS010000000
VERSION     DAZGXS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            95..1000
                     /locus_tag="ACHKG5_003793"
     CDS             95..1000
                     /locus_tag="ACHKG5_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0869803.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1034..1636)
                     /gene="cobO"
                     /locus_tag="ACHKG5_003794"
     CDS             complement(1034..1636)
                     /gene="cobO"
                     /locus_tag="ACHKG5_003794"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0869804.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1646..3298)
                     /locus_tag="ACHKG5_003795"
     CDS             complement(1646..3298)
                     /locus_tag="ACHKG5_003795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0869805.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGGLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3406..4686)
                     /locus_tag="ACHKG5_003796"
     CDS             complement(3406..4686)
                     /locus_tag="ACHKG5_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFP0869806.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4847..5164)
                     /locus_tag="ACHKG5_003797"
     CDS             complement(4847..5164)
                     /locus_tag="ACHKG5_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFP0869807.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5161..6531)
                     /locus_tag="ACHKG5_003798"
     CDS             complement(5161..6531)
                     /locus_tag="ACHKG5_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFP0869808.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6535..7776)
                     /locus_tag="ACHKG5_003799"
     CDS             complement(6535..7776)
                     /locus_tag="ACHKG5_003799"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFP0869809.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7776..9221)
                     /locus_tag="ACHKG5_003800"
     CDS             complement(7776..9221)
                     /locus_tag="ACHKG5_003800"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFP0869810.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9240..10628)
                     /gene="glmL"
                     /locus_tag="ACHKG5_003801"
     CDS             complement(9240..10628)
                     /gene="glmL"
                     /locus_tag="ACHKG5_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP0869811.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10628..11077)
                     /gene="glmS"
                     /locus_tag="ACHKG5_003802"
     CDS             complement(10628..11077)
                     /gene="glmS"
                     /locus_tag="ACHKG5_003802"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP0869812.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(11625..>11786)
                     /locus_tag="ACHKG5_003803"
     CDS             complement(11625..>11786)
                     /locus_tag="ACHKG5_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869813.1"
                     /translation="FGQQREGLIEINSDTVIFSILSSLIDSAISKFQHQIPVNGSVNR
                     ELLYEDYTD"
BASE COUNT         2843 a         2893 c         2925 g         3125 t
ORIGIN      
        1 ttcttataaa ttatttattg ctgctttggt taccattact tcgtaataag taatgtattt
       61 ttgatggttt aaactatttt taagggacgg cattatgcga gggaaaatac ctaaaacgga
      121 actgctggtg acttttgagg tcgtcgcacg tcatgaaagt tataccagag cggcagagga
      181 gttagcgtta acccaaagtg cagtattcag acaagtaagt gcgctggagg aatttttacg
      241 tactccgtta ttttcacact ctaaaaaacg catttttctt aatgatacag ggaagtatta
      301 cctggggata gtaaaagaaa cattaaataa gctggaacgc gataccaata ccattatgac
      361 ctggcagcct acggttcagg ttattgaact tgccgttaat cctactttta gtacccactg
      421 gttaatccca aatttacatg aattcacaaa gttacatccc gatattattg ttaatattca
      481 ttctttagct aataacggcg attttcttaa tcgggagtac gatgctgtca ttatgcgcga
      541 aaatttttgc gcgccctggg cggaagtaga atatctcttt gaagaggaga ttttgccggt
      601 atgcagcggc agtttgcttg caatgtccga tcaaaaactg tcagtggcag aactgcttac
      661 tgagttacca cttctgcatc aaagcacccg tattactggc tgggaagagt ggtttgcttt
      721 atctggcgtt agcagtccgc tggtcaacaa cggaccacgt ttcgatttgc tttcaatgct
      781 gattgccgcc gtgcgctcta atctcggcgt ggcgctgtta ccacgtttcg ctattcaaca
      841 tgatttggat agtggcgata tggtgattcc ctgtgatgtg cctattcgta ccggtaatcg
      901 ttttattatg acgtggcagg aagagaagtc tgactcaccg cacttgcagc aatttcgtga
      961 atggttgctg gcgaaatcgg tcgtaccgca ggagatgtaa aacctgcgcc tggcgggatg
     1021 ccaggcgcag ttattaccag tcgatacccg gctgggcctg aatgccgtta tcaaaagcat
     1081 gtttgacggg gcggatttcg ctgaccgtat ctgccatttt taatatctga ctgtggcaac
     1141 cgcgtccggt gacaatgaca ctttgttgtg ccgggcggtt ttgcagagaa gcgatcactt
     1201 cttcggtatc cagataatgg taagccagca tataggtcag ctcatcaagc accactaagt
     1261 cgtaacgctt gtcagccagc atccgtttgc tttccgacca gacctctttc gccgcgtcga
     1321 tatccgcttg ccggttctgt gtctcccagg taaaaccggt tcccataata tgaaactcaa
     1381 cccccagcgg ctggagaagg ttgtattcgc cgttgtccca ttgccccttg atgtactgcg
     1441 caacccccac cgttttgcca tgacccaccg cgcgcgtcac agtaccaaac gcggcagtgg
     1501 atttcccttt gccgttgccg gtgaaaacaa tcaagatccc ttttttctct atcgccgccg
     1561 cgacgcgcgt atcgacctgt tccttcaatt tctgttggcg ctgcacatgc cgttcagttg
     1621 aaattcgcgc ttccattcat tttccttaat gtcccgcagg gcaactggcg cagcggtgtt
     1681 gctgctcaaa ctgactaaag aaattattgc ctttatcatc taccaggatg aatgcgggca
     1741 gattttccac ttccatcatc cacaccgctt ccatacccag ctccgggtat tccaggcaac
     1801 gcagactttt gacatattct tgcgccagta acgcagctgc accgccaata cttcccagat
     1861 tgaagccgcc atgtttgtga caggcatcgg tgacctgctg gctgcggtta cctttcgata
     1921 acatcactag actgccgcca gccgcctgga aggtatcgat ataaccgtcc attcgaccgc
     1981 ccgtggttgg acccagtgaa ccgcaggcca tgttctccgg tgttttcgcc gggccagcgt
     2041 aatagacaat atgatgcttc aggtactccg gcatcggttc gccgctatcc agccgcgctt
     2101 ttattttggc gtgggcgata tcgcgcgcca ctacgatggg accacttaac gacacgcggg
     2161 tgcctactgg taatctggcg agatcctgca tcacgtcacg caacggacgg tttagatcca
     2221 gttgtacatg ttgcgcgtga ttttcttccc gcagtgaggc tggaatatat tgccccggat
     2281 tgtgttcgag tttttccagc cagataccgt gtttgttaat ttttgctttg atattgcgat
     2341 cggcagaaca ggagagcgcc atagctatcg ggcaagagcc accgtgacgc ggcagacgaa
     2401 tgacgcgaat atcatgagcg aaatatttac ccccaaattg cgcaccaata ccaaattgct
     2461 gactggcctc gagcaacact ttttccagct caatatcacg gaatgcttgt ccctgctcgt
     2521 ttccgctggt gggcaaattg tcgtaatatt tcgttgaggc cagcttggca atttttaagg
     2581 tttgatcggc tgaaagccct ccgacgacaa aggcgatgtg gtaaggcggg caggcagcgg
     2641 tgcccagcga tttcattttt tcgattaaaa atgcggtcag tttttctggt tgcaataagg
     2701 atttggtttc ctgataaagc gcggctttat tggcggagcc gcccccttta ttaacacaga
     2761 gaaaatggta ctcatcacca gcgacagcgc tgatatcaat ctgcgccggg agattggttt
     2821 gcgtattgac ttcggtatac atatccagcg gcgcattttg tgaaaagcgc agattatttt
     2881 cctggaaagt actgtaaatg cctttactta aggcttcggc gtcgttacct ccggtccata
     2941 tctgttgccc tttactggca acaatagttg cggtgccggt atcctgacag ttgggtaatc
     3001 ccccttttgc tgagacttca gcattacgta ataactgcaa cgcaacgtat ttatcattac
     3061 tgctggcctg ctgatcgtta agaatgctgg cgacctgttg caaatgagcg gaacgtaaga
     3121 aaaaagaggc ttcataaaat gcctgccggg caagcagcgt taatgcttca ggagcgattt
     3181 ttatcacttc ttcgccgtct aattccgtta ctgtaatatg ttgatcgcta attaatgtat
     3241 attctgtacc gtctttattt tgtagaaacg gttcttgcca gataaatggt ttggacatat
     3301 ttcacttttc accttaaacg ttagtgttac ttaattgccg tctaacttca tgatccggct
     3361 tataaaaatg cgtagtcatt ggcttctgat tgcttaagat ccccctcagg gcagagcaac
     3421 gttcgattca ttgggtttta atgctttacc ccgacagccc tcgccgaaat gttcgccttc
     3481 ccaccgactc accatcgcag cgcccattgc gttgctcatg acgtttgtcg cggaacgccc
     3541 catatcaagg aacggatcaa cgcccatcag caagatcagc cccgcttccg gaatattgaa
     3601 ctggttgagc gtggcggcga taaccaccat tgaggcgcgt ggtacgccag ccattccttt
     3661 cgaggtcaac atcaggatca acagcatggt gatttgctca ccgatggata aatggatatt
     3721 gcaggcctgg gcgatgaaaa ctgtggcgaa ggagcagtag gccattgatc caacgagatt
     3781 aaatgagtag ccaatgggta agacaaagct ggcaattttg ggggaaacgc caaattgctc
     3841 cagtttttca agcgttcccg gaaaagccgc ttcagaactg gatgtggtaa atgccagcag
     3901 ggcgggttcc gaaagggcgc gggtcaggcg tctgatgcag gggccgacat aaacgatggc
     3961 cagaccgata agcagtaccc aaagtaataa catggtgaaa taaaattcac ccataaagat
     4021 cccggcgctc accataactg ccagtcctcg ttcagcaatc aatgctgaaa tagcagcgaa
     4081 tacggtcagg ggagcgaaga gcatgacgta gccagtgagc tttaacatgg catgtgccag
     4141 cgaatctaag gcgtgaacga tggcgctgcc tttctcgcca atcgccgtca ggctacagcc
     4201 gaggaaaatt gagaacacca cgatttgcaa aatttcattg tgcgccatgg catcgacaat
     4261 gctggtgggg aaagcatgcg aaataaatac ttttagcgta aagggttctg ccgcgaccac
     4321 tccggtggtt tcggcaccgt gtgcaacaaa attaattcct gtacctggca tgaagaaatt
     4381 taccgttatc aagcctaagg caattgacag caatgaggcg caaataaata aaaagagtgt
     4441 tttagaaaaa atacgaccaa gggctttggc atcgcccatt ttagcaatac ctaccaccag
     4501 ggtagagacc actaacggag cgataatcat ttttatcagt cgtaagaaaa tcgtcgtgaa
     4561 tatcgatatt tcttgcgcat agagttttgc ggtttccggt gaagcagtgt tattgagcac
     4621 tacgccgata atcattccaa gtaccagcgc caaaagaatc atcgtggtta aacttatttt
     4681 cttcattttt tcctccatca ggtgcacggc gataaatcag aatcaggaga agtagatgcc
     4741 tgaatagaga ttcaggcatc tacctgctat tacaacctta caaatagctg aagtgttgaa
     4801 ctgcaataag tgacgggatg tcagttggct aaaagtattc ggatttttac aaatcgaaac
     4861 tcatatttaa taaaggtgca tgttgctgtg caccatagac atcattgtca cctacattcc
     4921 cggagataat atctcgtggc atgacgattt ttactgcgtt agaaggatca aaccagacaa
     4981 tacgatgaat aaagtcaggt tcaacatgat ataagcgtgc aatcagttct ggggttaatt
     5041 gccctgatga tttaactcgc tcatagtttt ctttcgtttt aaataaaata tctaaaacca
     5101 gttcgtaagg tccggcattt ttcgaacgaa tgacttgcgc cagtgagaca attgactgtt
     5161 tcatgcctga actccttctg gcgtcacctg ttcaatatgg aaatcaaaac gcagagcgtc
     5221 gttggcttca atcaggtgat atatggaaaa ttcgtatacc ggcccgccct ggatatcaga
     5281 aggggagaac gggaacgcca gattacctgc ggtagcgatg cggttttcat agccgtagtg
     5341 cagcatggta gagcgcacca gcgagcaaac gctgttggca atttcctggg tcggtgcaac
     5401 tacatcaagg acaatcccca gctcatgccc ggcagtttgc atcggttcat ggtctcccat
     5461 caccccgttt ttgccgtaca ggtggaaatt gatgcgaatg ctgtcatcgt cgagcgacag
     5521 gttacgcgaa acgctggttt tgacttcatc gatgatttta tcgatcccgg cgatcataat
     5581 cgggtcgcgc gttccggcga tggtcagaca gcggaagccc acccgtcgtg caccttccag
     5641 tttcagggca tacggcgttt cttcatgctt agaaccgctg acgtagactt cgccgtcatt
     5701 tactgcttta aatgtgcagc ctttcaggtt caacacgccg ccaggtcctg gcaggaagta
     5761 gggatcggat ttctcataca gtgtgtgtgc agctgctgac gtttcggtaa atttacgctt
     5821 cggattaaat gttttcagcg taaagccgtt gtcatcaatg atgcccatcg cacagtcaga
     5881 gcctgagccg ggcgttgcgg cgattgccgc gcattcaagg attttcccac aatgcagcgc
     5941 cagaccttca tcaaagcctt gcatgatcgg gagcgcagca aagcaggccg ggtcgtaagc
     6001 gcgcccgccc agcaccactt gcgcgcgttc tttcagcgcc cgctggaagg gttcgatgcc
     6061 catttgcgcg acgatatacg tactttcgtc aatcgcgtcg tgggttaacg gcgggacaaa
     6121 atccagcgcc gtgattttgc cgttatccag cgcctggtgg acgatggctt tgtcaacgtc
     6181 tgccgggatc aacgccatcg agaacgagag gtgctcttcc cgggcaatct catgaattat
     6241 ttctcggcac cactccaggt gtggtgcggc accagaaccg ccagccgttc cgatcaccac
     6301 cggtatatta ttttgcacgc ctgcggtgat catatagcgc agatcgcgct tgacccctgc
     6361 gcgatcggta aacggtttgc ctgccccgag gtagtggggg cctggatcgg aagagcctgc
     6421 atcaacggcg atcagatcgg gcgattctgc catcgctttg cggaagctct cttccgggaa
     6481 tccatacccg aggattgccg ttggcgataa gactttaaaa gaacgagcca tttgttaatc
     6541 cttagcctgc aacagcgcga tagtacgatt catttcgttg aagacgatat cgaggccttc
     6601 gtcaaagccc atccctggtt ttaccagcat acgcatgggg cgagcggcaa gggcgacgtg
     6661 gacacaggtg cgggcactga catcagtttc attgcaggtg ccgccctggt acgcttccat
     6721 gctgtggctg ttgcagtaaa gaaccgcatc gacgatgttg tgaatactgc ccagatccgg
     6781 ggttttgatt tgcaccatgt gacaactggc agcatcagtg aaatcaacaa tatcctggta
     6841 ggtgttacac cattcatcgg ccacaatttt cacgccggaa ccgaggcgcg tcagctcttt
     6901 ggtaatcgcg gtcagcaggc gaatttgatc gggcttgtta ccggcatcga ccggcccttc
     6961 gatgtagagc ggcaggcctt gcgcttcttt ttccaggctg gcgatgtatt gcgcacagcg
     7021 aagcggatcc atatcgaaga tcagaccgat agtgccgtat acatcgatgt gcagggtagg
     7081 gtggtagcgt gcgctggtgc gcttgcttag aatgcgatcc gacaaccagc ggacatattc
     7141 gcgcagtttt tcacctttaa agcccagctt ctcttcgacg ttattaatca gcgcatgggg
     7201 cagcacgtcg atgcctttaa ggatcatctt atcgacggcg atatatcgat cgtcgccgct
     7261 ctgaccaaat aatggaatgg attccgccac gcgtggcaac tgccattcat cacagaccac
     7321 ttcagttttc agacggccgg ttgccagcgc ggtagcatca agcagcgcct gtgataatcc
     7381 gtagcgcacg gcggtatgca gcaagttgcc gtcaatacgc aatttgtcga agaaacgggc
     7441 attcggcagg aaagcatcca catcgcggcc taccagtaat ggcttgatat ggtcgttgag
     7501 gaacggaata aagtgctctg cgaggaacag tggatcgcga ccgcctgccc cggaatactg
     7561 tacggcagta cagtcaccta cggcgaccgc gccgttttcc agaatcaact gtaccgatac
     7621 gcactccccg gcctgacgta cggcgttaaa cccctgtgtt actggctccc cggtataaaa
     7681 aaagccgtca tgacccgctc cgttttttat cgcctgctgg tcatcgaaat agaatgagga
     7741 gtagccagcg gtgaacagag cctgttttat tttcattatg gtcttcctat taatttactg
     7801 tgggatacag cattgatgtc atctacaacc atctggaatg tcggttggcg tccttcacag
     7861 cgtgcgcgtt ccgcaacgta atcgtggtgg aggtcgagaa tatcttttgg caatggaacg
     7921 gctccggctt ccagtacgcg tatcgcgccg gtgttgtcac ggaccggaag aattttgccc
     7981 gcgttgcagg cggctggcgc aaacggcaca tccagtacgc ccgcttcaaa tgccagcacc
     8041 gttccgcgcg caatatcacc attacccagt tcgaacactt tattcagtac cgcgcgaacc
     8101 tcgctcttaa tcagttcaat ttccagttca accgcagggc acggcgggaa tttctgctca
     8161 ttgaccatgt tgagcatttg gcgggaggct ttcagcccct gaatgttggc agcggcagtc
     8221 gggatacccc aggcttcgtg cggacttttg gtgatgactt tggtggcacc agacatcccc
     8281 gcgacggctg cgccccagga gataatagcg aacgctttcg attcgtcttc cggaaagccg
     8341 cccatccact ggtgaaacac ggtactcaat tcatagtcgg tatagccata gctctgaaaa
     8401 tattcgtgtg caagttcacg cagagactgg atcgccgcaa catcctgcgt caggctgccg
     8461 acctggccat aaccaacggt gatagatttc acaccctgtt ccagcgccag caagccttcg
     8521 attatcgcga tagagtgcga gataaacggc gggatcagcg tacccgttag tgggccaaac
     8581 ggttcgcggt tgatgcggat cccgtgttct tcatacatcc ccattaaacg gtcgcagtac
     8641 tgccagtcgc ggatcgattt ttccagcgtt acgcgtttgg catacggaat gttgtaggaa
     8701 atgccaccgc cttcataact ggtaaagccg ctggccattg agatttcagc cagcagacgc
     8761 gcatctggcg tgccgtggcg gatctgtagc ggtttttgca gggtttcggt gaggcgacgg
     8821 caagctgcga caccatggtt aactaccggt aaaccgttca gtttagaggt tccggcttcg
     8881 atggattttt taatccctac agccgcttct tcgtaccgat tcagacgggt atatgcatcg
     8941 atggtgcttg gcagcagatc gcactcttct tgtagtgttt tcagcagttc gatgtgttcg
     9001 tccatcagcg ccacacctgc gcgcggttgg ctaagggttt taccttcctt gtctgctttc
     9061 agcagcgcaa gagagaaacg tttatgctca gggatggttt gttggtattt aacgccatct
     9121 tcaaagtttt caacatcctt gccggtttcc caggttttca gtacctgttg ccgctcggtc
     9181 atgaattcgt catgggttaa tttcttattt cgaagttcca ttgattgcag ccttaattgt
     9241 tatagggtta agcattgaat actggtgcgc gcggcggcca gcggatcgac tctggcgacg
     9301 tttgccagca gtggtaatag cccccgggaa tcgcggtaat attcaaattc ggtcggtaag
     9361 agaatgcgtt taccgtcgtc gtttaactcg cggtatttca accagtgatg catatcgaac
     9421 tgactggcac gcgagagcca tccgccggat ccgacgactt tacgcacggt tgtcaggtca
     9481 cgtcccatct gtaaatcgac attgccgacg caggtgcaga cctctttttt ggtgcccgca
     9541 tgtctttcgg tggcataacc cacacacagt ccggccagga cggtatcgaa atatttctct
     9601 tctgcatttg cgggtaagta gtcgggatga gcgactagat gacggaggta atgataaaaa
     9661 gcgtccagct gtgcgggttg ctgagcaaaa ttcacattga ctaactcttt cgcactttca
     9721 ccaaccacca ctgccgagac gcgcattccc agatcgcctt ccaccgtccg ttttacgaag
     9781 ggttccggta cgccgtgtaa aacggtgtcg ggcgagagcg tgttggcaca ggcggaataa
     9841 acatcggtgg ttgcgccgcc catgtcgatc agcatgaact ctttccagct gtgatcgacg
     9901 ttactgattg ccttgaccag ttcaaagacc gtccatggtg tcggcattgg ttcttcgcct
     9961 gttttatcaa caatgacatc cagccctttc cctttaacga tgcgtttaag gaatatgtcg
    10021 caaatagcct ggcgtgcagc tacagggttc ggatggtcaa gatcgggcag aacgttgtcg
    10081 acaatggtca ggtctttatg cccgaggatc tcctgcacct cgtcctgaat atcccggttt
    10141 ccggcataaa taatggcgca gtcgagtttg gattctgcca gcacacgggc gttattcagt
    10201 ccgtagcttt cttctccgcc gtcggtgccg ccggtgaaca agagaatatc gggctgcgtt
    10261 tcttccagtg cctggatatc acggcggttc agcttgtagg cgtaatattg cgcaatttta
    10321 gcacccgcag agtgagcggt tactttcgcg gtttccaggg tgatagaagg caccaatccc
    10381 attgcggcaa ccgccagccc acctttggca gaggaggaat acttcaatgc aacctcaccg
    10441 ctgtttaaca gggggagggc attatctacg tttaacacct ggttcaggct ggagaaaaac
    10501 cccttcgcca gatgatgggt ggtggttggg gtcagtacgt ggttaaccaa cgttaacgcg
    10561 tccccctccc gggtaaagag ggctgctttg gtccacgtcg atccaatatc gacagagacg
    10621 atttgcatca gatagcctct tcaagaatac gagtatcaac gtcatgacgt tggttgatat
    10681 cgtgggccat cagctggcaa acatcttcta agtcatgact tggggcaaag acgcgatcaa
    10741 atcccatctc tttgaatttg gtttccacgt cagcgaagtc atgttttccg accaccaggt
    10801 taccgcccac atagagcagg atgtccccca gtccacgctc gatgcagcgt tcgcgcatgc
    10861 ccagacagtc gatatcgcca tgcccataga tagaggagac gacaatcgcg tcagcgccag
    10921 tttcaatggc agcatcaata tattcgtcct ggctgaccat tacgcccaga ttaatgactc
    10981 tgaagtcgtg gttgctaaaa acgcggtcca gaactttatt gcctactgca tggcagtccg
    11041 cgccaataac gccaatcaca agtgttgctt tcttcatggg taatcctttg taagacagag
    11101 agtaaaataa gttacacacg tcgtatttat ggccgttcat aacggcacag gtttaatatc
    11161 acaggagtgt ctttatctga ccgtaatgtt gcaattgatc tattttcaaa atgtctttcg
    11221 ggaaatgatt gtttgaagtg tgtttttaaa tttccatgtt aattcagtgt gttatgtcaa
    11281 ctaatgtcat gagtgttaaa tttttaaaat taacttgatt taatgtattg agataattgt
    11341 gatctacttc taatttgcat ttttgtgacg tggttttagc catgagtaaa tgcatttcat
    11401 aagatttaag tcgaatttca ctgcaattca tctctgctta atgtaaaaat aatgttgaaa
    11461 ataacaaatg gttaatgatt catgcgaatt atgaccttag ttagctcttt taatttttcg
    11521 taaaaaagag aggtacatga aggtgctatc agtatgagta acgttaatca aaggttttat
    11581 tacgtctggc aataacgcag gccatttttc gcgacagggt ggatttaatc agtataatcc
    11641 tcatacaata gttcacgatt aactgaaccg ttaacgggga tttggtgttg aaatttagaa
    11701 atggcggagt caattaatga agacaatatc gaaaaaatga ctgtgtcaga attaatttca
    11761 attaatcctt ctcgttgttg accaaa
//
LOCUS       DAZGXS010000127        11757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGXS010000127 DAZGXS010000000
VERSION     DAZGXS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            238..4221
                     /gene="ehaA"
                     /locus_tag="ACHKG5_003804"
     CDS             238..4221
                     /gene="ehaA"
                     /locus_tag="ACHKG5_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP0869814.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4363..5451
                     /locus_tag="ACHKG5_003805"
     CDS             4363..5451
                     /locus_tag="ACHKG5_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0869815.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5493..6424)
                     /locus_tag="ACHKG5_003806"
                     /pseudo
     CDS             complement(5493..6424)
                     /locus_tag="ACHKG5_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6516..7023)
                     /locus_tag="ACHKG5_003807"
                     /pseudo
     CDS             complement(6516..7023)
                     /locus_tag="ACHKG5_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7281..7886
                     /locus_tag="ACHKG5_003808"
     CDS             7281..7886
                     /locus_tag="ACHKG5_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0869816.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7926..8789
                     /locus_tag="ACHKG5_003809"
     CDS             7926..8789
                     /locus_tag="ACHKG5_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0869817.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8779..10326
                     /gene="fdrA"
                     /locus_tag="ACHKG5_003810"
     CDS             8779..10326
                     /gene="fdrA"
                     /locus_tag="ACHKG5_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0869818.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGTDNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10326..11744
                     /locus_tag="ACHKG5_003811"
     CDS             10326..11744
                     /locus_tag="ACHKG5_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0869819.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3109 a         2726 c         3179 g         2743 t
ORIGIN      
        1 ggtattagag actggggggg aatgtaatga taaagttaag agtgttaact taaaataagt
       61 ttttagtagt aacttgctat tttattaccc tctgaactac atactcagat atgtggttgc
      121 caatgctatg caaaattttt gaatttttat atgcgatgaa cagtaaccgg aatggcattt
      181 aatgcgttgc tgtttatgca atcttggttt tatttggatg tattgctgga gattgtgatg
      241 aataaaatat atcggctaaa gtggaacagg tcccgtaact gttggagcgt ctgctcggag
      301 ctggggagca gagtaaaagg aaaaaagtcc cgggctgttt taattagcgc gataagttta
      361 tattcatctc tggtattcgc cgatgatgtc atcgtaaacc aggataaaac tattgatttt
      421 ggcaaagaga accagagcat cgattaccgt attacggtga cagacaatgc caatctggta
      481 atcaatgcga cagatacttc ccgtccgcgt ctgactctcg cttctggtgg tgggttggat
      541 attaccggag gaaaggtaac aatcaatggc ccacttaact ttttgctgaa aggtacgggg
      601 ttcctgaatg tctccaatgc tggcagcgag ttatatgctg atgatttgta tgaatcaaac
      661 tcaggcatga gacacgatcg cggctatttt aatgtctcca acggcggcaa aatccatgtt
      721 aagggcacca gccgtctgac ctatttgcag ggaaatgtca gtggtgaagg tagccaggta
      781 aattccgaaa ccttctttat gggcgtttac ggcagttacg gtggtaatca gtacctgtca
      841 gttaataacg gcggtgaagt taatgccagg aagcaaatta gcctgggcta ttatgatcaa
      901 gtctccgata caacacttgc tgtttcggaa ggtggtaaaa tttctgcgcc tactattagt
      961 ttaagcacca actctgagtt agcgttaggg gcacaggaag gaagcgcagc gaaggcagca
     1021 gggattattg atgccgaaaa aattgagttt gtgtgggcaa agacatccga gaagaaaatc
     1081 accttaaacc acacggataa agacgcgact atttccgcgg atattgtcag tggcagcgag
     1141 ggcctgggct atatcaatgc gctcaatggc acgacttact taaccggtga taactctgcc
     1201 tttagtggta aagtcaaaat tgagcaaaat ggcgctttag ggatcaccca aaatataggt
     1261 acagcagaga tcaacaaccg cgggaaatta cacctgaagg ctgacgatag catgaccttt
     1321 gccaataaga tctctggcaa cggtacaata agtatcgaca gtgggacggt ggagttgacc
     1381 ggcaataact atgcattcag cggatatatt gatgttgctt ctggtgctgt cgctgttatt
     1441 tctgaagaca agaatatcgg tcgtgcagag ctggatgtcg atggcaaatt gcaaattaat
     1501 gccaacaaag attgggtatt tgataacgat cttgaaggta gaggcattgt tgaaataaac
     1561 atggggaatc acgaattctc cttcgatgag tttgcttata cagactggtt ccagggttca
     1621 ctggcgttcc agaacacgac atttaatctg gaaaagaatg ctgagtttct gcagaaaggc
     1681 gggatcactg cgggtcaggg aagcctggta acagtgggta agggcgctca ctccattagc
     1741 actttgggat tctccggcgg aaccgttgat tttggtgccc tgacagcagg tgcacagatg
     1801 acagaaggga cggtcaacgt tagtaaaacg ctggatttgc gcggcgaggg tgtgattcag
     1861 gtttctgaca gtgacgttgt ccgctcagta tctcgtgata ttgactctgc gttatcgctc
     1921 actgaagtcg atgatggtaa cagcaccatt aagttggttg atgcgcaagg tgcggaagtt
     1981 ctgggcgatg cgggcaatct gcaattgcag gataaaaatg ggcaaatcct ctccagcagc
     2041 gcccaacgtg atattcagca gaatgggcaa aaagcggccg tcggcactta cgactatcgt
     2101 ctgacgagtg gggtaaacaa tgacggtctg tatattggtt acggcctgac ccagcttgat
     2161 ttacacgcta ccgacagcga tgctctggtg ctgagctcta acggtaaaag cgagaatgcc
     2221 gccgatctca gcgcaaagat taccggcagt ggtgacctgg cattcagcag ccagaagggt
     2281 cagaccgtat cgctttctaa caaagacaac gactataccg gtgttaccga tctgcgcagt
     2341 gggacgcttt tgttgaataa cgataacgtg ttgggtaata cccatgaact gcgtctggcg
     2401 gcagagactg aactggacat gaatggtcac agccagaccg tgggcacgct caatggcagc
     2461 gccgattcac tgctgagctt aaatggcggc agtctgacgg ttaccaacgg gggcacttca
     2521 accggttcgt taacggggag cggagagctg aatattcagg gcggcacgct ggacatcgcg
     2581 ggcgataaca gcaacctgac ggcgaatgtg aacattgcta attcggctaa tgtcctggta
     2641 agtcatgcgc agggattggg tagcgcaaac gttgagaaca acggtaccct ggcgttgaat
     2701 aatagcgctg aaaaaagagc ggctgcgtct gtgaattacg ccctgggcgg caatctgacc
     2761 aacaacggta cgctgatgac cggaatgtca ggacagcaag ctggcaatgt gttagtggtg
     2821 aaggggaact accacggtaa taacggtcaa ctagtaatga atacggtact gaatggcgat
     2881 gactcagtaa ccgataaatt ggttgtcgag ggcgatacta gcggcacgac tgccgttacg
     2941 gtgaataacg ctggcggtac aggtgcgaaa acccttaacg gtatcgaact tatccatgta
     3001 gacggtaagt ctgagggcga atttgttcag gctgggcgta tcgttgcggg ggcgtatgac
     3061 tacactctcg cgcgtggaca aggggcaaat agtggtaact ggtatctgac cagcggcagt
     3121 gattctcctg aactgcagcc ggagccagac ccgatgccga atccagagcc aaacccgaat
     3181 ccagagccga accctaaccc gacacctacg ccgggtccgg atctgaatgt ggataatgac
     3241 ctgcgaccgg aggcgggtag ctacattgcg aaccttgcag cagcgaatac catgttcacc
     3301 acgcgtctgc atgagcgtct gggtaatacg tactataccg acatggtgac gggtgagcag
     3361 aaacaaacca ctatgtggat gcgccatgaa ggtggtcata ataaatggcg tgatggcagc
     3421 ggccagctga aaacccaaag caatcgctat gttctgcaac tgggaggcga tgtcgcgcag
     3481 tggagccaaa acggcagcga ccgctggcat gttggggtca tggcgggata tggcaacagc
     3541 gacagcaaaa ccatttcctc gcgaaccggt tatcgtgcaa aagcgagtgt gaacggatat
     3601 agcacaggcc tctatgccac ctggtatgcc gatgacgagt cgcgtaatgg cgcgtatctc
     3661 gacagttggg cgcagtacag ctggtttgat aacacagtga aaggggatga cttacaaagt
     3721 gaatcctata aatcaaaagg atttaccgct tcactggaag ctggatacaa acacaaatta
     3781 gctgaattta atggcagcca gggaacgcgt aatgaatggt atgttcagcc gcaagcacag
     3841 gttacctgga tgggagtcaa agccgataag caccgcgaaa gcaacggaac cctcgttcat
     3901 agcaacggtg atggcaatgt tcaaacccga cttggcgtaa aaacctggct gaagagccac
     3961 cataaaatgg atgacggtaa atcccgcgag ttccagccgt ttgtagaagt gaactggcta
     4021 cataacagta aggatttcag caccagtatg gatggcgtgt ctgtcactca ggatggagcc
     4081 cgaaatattg ctgagataaa aaccggggtg gaaggacagc taaatgccaa cctgaatgtc
     4141 tgggggaatg tgggcgttca ggttgccgat aggggatata atgacacctc tgcaatggtt
     4201 ggcattaagt ggcaattctg atctgaataa tgagtaaaag gagagagagc cgaacgggag
     4261 ataattcggc tctctgtgtc attttcttgc agccagcttt atccgtcaga gtgaagactc
     4321 gcagatttag ctggcgcaca agatttaatc aaggtgtgtt caatggcttc gcatgatttg
     4381 agtgtttttt tggaagagtt cggggcaacg gtgaatttga cgttgcctgg tatcgtgtcc
     4441 gaaaaagaac ggcttttact caagctgcta atggagggaa tgtcggttac agaaatctca
     4501 cagtacagaa atcgcagtgc aaagacaatt tcacatcaaa agaagcagct gtatgaaaaa
     4561 ctaggcatcc agagtgatat taccttctgg cgggatatct tttttcaata tcatccgcaa
     4621 gtcattagtg gcacgggaaa taaaaataat ttttatatcc ctgataaccg ttgtcaccat
     4681 atcgtcacgc ctgaagccat cagtctggcg ttggaaaacc atgaatttaa accgtggatc
     4741 caaccggttt tctgcgcgca gactggggta ctgacgggct gtgaagtgct tgtccgctgg
     4801 gaacatccac aaacgggaat catcccgccg gatcagttta ttcctctggc tgagtcatcc
     4861 ggtcttattg tcataatgac ccgccaactg atgaaacaga ctgcggatat tctgatgccg
     4921 gtaaaacatt tgctgccgga taatttccat attggtatca acgtctcggc gggttgtttt
     4981 ttggccgcgg gatttgaaaa agaatgcctg aacctggtca agaaattagg aaacgataaa
     5041 atcaaactgg ttctcgagct aacggaacgt aaccctattc cggtaacgcc agaagccaga
     5101 gcgatatttg acagccttca tcagcataac attacctttg cgctggatga ctttggtacg
     5161 ggttatgcga cctatcgtta tctgcaggcg tttccggtcg attttattaa gatcgataag
     5221 tcatttgtgc aaatggcggg tgtcgacgaa atatccggtc atattgtgga caatattgtc
     5281 gaactggcgc gtaagcctgg tctgagtatc gtggcggaag gggtagaaac ccaggagcag
     5341 gcggatttaa tgatcggtaa aggcgttcac tttttgcagg gctatttgta ctctccgcca
     5401 gtaccgggta ataaatttat ctctgaatgg gtaatgaaag caggtggttg atgtaaaccg
     5461 ctattcacag cgcatcggga ggttggcagc gattaattct cccgatgcag taaatcctga
     5521 tagatccccg ttaatatgcc atttggcgca aattgttttt taatccactg cgtcacctga
     5581 cccgtagcag aatgctgagt cgccagtaac atgcgtgaat cctggcgcgg gttatggatt
     5641 tgtcgggtaa ccagcaggga ttgcgccatc gcctcacgga ccatgtaatc tggcaaaaaa
     5701 acaatccctt cacctaaaat ctggcactga catttggtgt tgaaatctgg caccagaatt
     5761 gactcctgcc cgtgcagcaa ccagcccact tttttattaa tcgtatgcgc ggtgtcctcc
     5821 accataatat tagggtagag acgcagttgg ctttcggcga tggttccggc acaaaggcta
     5881 acgggtgatc cggggcgata gcaaaagccc agcgaatcgc gccaatttca gtgtaatcaa
     5941 taccgccgcc gtccagcagc gtgtcgggtg cgccgatagc gatatttgcc tgattattaa
     6001 taatcgcatc ccagacgccg ttatacactt cggtggtgac ggtaatctgg caggtaggaa
     6061 actgcttttt cagcacctgt aataagcgtg cggtgtgttt gggcgtataa agcaactgat
     6121 taatacaaat gcgcacacgc gcttctatgc cctgcgaaat agtatcgatg ctgcgtttga
     6181 tggcgtgaaa atcattcagc aggtcggtag ctttgcggaa aaaataacgc ccggactccg
     6241 ttaactcaat gctgcgggta ctgcgagtga acagcaccac atccagccct gtttccattc
     6301 gcttgatggt gtagctaatg gcggaagtgg ttaaacccaa ctcttctgcg gctttactga
     6361 aactgccaaa acgcgcagcg gtagtaaatg ccaacagatt ctcttcggta aaaattgagt
     6421 tcattaaacc attcccgcca ttatctaata tgaatttctc caagcataac tgtagaggta
     6481 tcaattagag atgggtaatc atttttgaat aacttttaat acccgtcgcg tttagtcgtc
     6541 gcttcgctta atttttgtcc gccatattct gaaattatcc ccacgcagtt acccgccaga
     6601 aaacccagta acgtcaccgt ccagttcatc ccgctggcaa aaaacagcgt catgccgaga
     6661 aagccaccgg gaataaatga cagtaaccag aaacgccctt gccagaccac caccgccgag
     6721 aagggtacgg tagtaattac gctcgcccag aacgcggaca gtccacttac agaggccagc
     6781 caaccggcag ccagcgcggc gaaaaacgcc cacaccatct cggaataatt aaccgacaga
     6841 cttctgataa accccgattt accgccgcct gcggcataaa aactgcaaaa ggcgacgaag
     6901 ccaatggttc ctaataattc ctaataattc ccatccctga gagagcccga catgtgaaga
     6961 cactaactgc caaagccctg cacaaatacc gacggtgaca cccgttgccg tgagtccgtt
     7021 catcttgttc ccctctggtt agacatggac gaaagctact cttacatgtg cagactatag
     7081 agaaataaaa cctgattgag tgaaagggta tcgggtcaaa gaaacaaata ttgcactacc
     7141 gcacactgcg aaaagattgt tgaacatcat tcaacaaaaa ggcgaaatac ggcgaattaa
     7201 atttaagcgg gttcttttat cagattatcg ttgctattct caattcatcg aaaacagcca
     7261 caaaaaccag gggaatgtga atgagtataa aaaatctacc tgccgactat ttattggctg
     7321 cacaacaggg tgatatcgat aaagtaaaaa cctgccttgc gctgggtgtc gatattaata
     7381 cctgcgatcg tcaggggaaa acggcaatta cgctggcaag tttatatcag caatatgctt
     7441 gcgttcaggc attaattgat gccggagcgg atattaataa gcaagatcat acctgtttaa
     7501 atcctttttt aattagctgt ctgaacggtg atttaacgct gctacgaatt attttaccgg
     7561 ctaaaccaga ccttaattgc gtcacccgtt ttggcggtgt cggcctgacg cctgcctgtg
     7621 aaaaaggcca tttaagtatt gtaaaagagc ttttggcgca tacggaaatt aacgttaacc
     7681 agaccaacca tgttggctgg acgccactac tggaagcgat tgtgcttaac gatggcggga
     7741 ttaaacagca ggcgattgtg cagttattgc tggaacacgg tgccagcccg catctgaccg
     7801 ataaatatgg caaaacgcca ctggaactgg cgcgggaacg gggctttgaa gagattgcgc
     7861 agttactgat tgccgcaggt gcataaaccg ggaggcttgc tatcaacaca ccagaaaaac
     7921 ggtgtgtgtg ggcgctaact gcggatgcgg attttctggc gcagcgaggg caaggacagg
     7981 ttgaacaggt ctttgccaga gcggtaaata tcgcactccc ggctcgccag cagttgctga
     8041 cgctgctttg tgaagagtac gacaatgcgc caaacagttg tcggttggca ctcactcact
     8101 ttgatgatct gttccggcat ggtgataagg ttcagtttga cgatcaaggt attacggttg
     8161 gtcaacatct tcatatagag atgagtcgtt gtcggcgttg gctgtcccca accttgcaaa
     8221 tgaccgctgt gaattttcgc cttatcgcct ggcaacagtg gcacgacatc attcatcagc
     8281 acctggggga aaatgaaacc ctgtttaatt atcgcggcga taatccgttt tatcaggcgt
     8341 taaataaaga attacatatt aaacgacggg cagttataca ggcagtaatc gataaacaaa
     8401 atatcgcctc agcggtcgcc agtatgatgg gcttagggat tggccttacg ccatcagccg
     8461 atgattattt aacaggtctg gcacttattt tatttatttc cgggcatccg gcgggaaaat
     8521 acaaagagga attttatctc ggcttgcaac gcggcagaaa taataccacg ttattaagtg
     8581 ccataacgct ggaagccgca ttacaacaac gctgccggga aaatattcat cgttttattc
     8641 acaacattat ttatgacatc cctgggaacg caactcaggc aatagaaaaa attaaacata
     8701 ttggctccag ttccggctgc gacatgctgt atggcatggc cgatggttgt gcgctgagcc
     8761 aaacctacgg agggaattat gtcagttaaa atagtcatta aaccgaatac ctattttgat
     8821 tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac tcgacggcgt cgagcaggca
     8881 tttgtggcga tggcgaccga aatgaacaaa ggtgtgctga aaaatttagg actgctgacg
     8941 ccggagctgg agcaggcgaa aaacggcgac ctgatgattg tcatcaatgg taaatcgggg
     9001 acggacaacg agcagttgct ggtggagatt gaagaactgt tcaacaccaa agcgcaaagc
     9061 ggctcgcacg aggcgcgtta cgccactatt gccagcgcca aaaagcatat cccggaaagt
     9121 aacctggcgg tgatttcggt caacggtctg tttgccgctc gcgaagcgcg tcaggcgctg
     9181 caaaacgatc tcaacgtgat gctgttttcc gataacgtct cagttgaaga tgaactggcg
     9241 ctcaagcaac tggcccacga aaaagggctg ctgatgatgg ggccagactg cggcacggcg
     9301 attatcaacg gtgcggcgct ctgctttggt aacgccgtgc gtcgcggcaa catcggtatt
     9361 gttggcgcat ccggcaccgg cagtcaggag ttgagcgtcc gcattcatga atttggcggc
     9421 ggcgtttcgc aactgattgg caccggcggg cgcgacctga gcgagaaaat cggcggcctg
     9481 atgatgctcg acgccatcgg gatgctggaa aacgatccgc aaactgaaat cattgcgctt
     9541 atctccaaac cgcctgcgcc tgcggtggcc cgcaaagtgc tggaacgtgc gcgcgcctgc
     9601 cgcaagccgg tggtcgtttg cttcctcggt cgtggcgaaa cgccagtgga tgagcagggg
     9661 ctacagtttg cccgcggcac caaagaggca gcgctaaaag cggtgatgct ctccggcgtg
     9721 aaacaagaac atctcgacct gcataccctg aaccagccgt tgattactga cgtgcgcgct
     9781 cgcctgaagc cgcagcagaa atacattcgt ggcctgttct gcggtggcac gctgtgcgat
     9841 gaaacgatgt tcgcggtgat ggaaaaacac agcgatgtct acagcaacat ccagcccgat
     9901 ccggaattcc gcctgaagga tatcaaccgc agcatcaaac acaccttcct cgactttggc
     9961 gatgacgact tcaccaacgg caagccgcac ccgatgattg accctaccaa ccgcatcagc
    10021 cgcttgatcg aagaggcgcg cgatccagaa gtggcggtga tcgtgatgga ttttgtgctc
    10081 ggatttggat cgcatgaaga tccggtcggt tccaccatcg aggcgatcaa agaagcgaaa
    10141 gcgatcgccg ctgccgaagg acgcgagttg atcattctcg cctatgtgct gggtaccgat
    10201 cttgatacgc catcgttaga acaacaaagc cagatgctgc ttgatgccgg cgtgattctg
    10261 gcgagcagca gcaccaatac cggattgctg gcgcgtgaat ttatctgcaa aggggaggaa
    10321 gcctgatgag ccagtcactg tttagccaac cattgaacgt tattaacgtc ggcatcgcca
    10381 tgtttagcga tgacctgaaa aagcagcatg tagaagtgac tcaattcgac tggacgccgc
    10441 cggggcaggg caatatgcag gtggtgcagg cgctggataa cattgccgat tcgccgctgg
    10501 cagacaaaat cgccgccgct aaccagcagg cgctggagcg cattatccag tcgcatccgg
    10561 tgctgattgg ttttgatcag gcgattaacg tggtgccggg catgacgccg aaaaccattc
    10621 ttcacgccgg gccgccgatc acctgggaaa aaatgtgcgg cgcgatgaaa ggcgcggtca
    10681 ccggagcgct ggtgttcgaa ggactggcga aagatctcga cgaggcggct gaactggcgg
    10741 cttccgggga gatcaccttc tcgccgtgtc acgagcacga ctgcgtggga tcgatggcgg
    10801 gtgtgacctc ggcctcgatg tttatgcaca tcgtaaaaaa caaaacctac ggcaacatcg
    10861 cttataccaa catgagcgag cagatggcga agattttgcg tatgggcgct aacgaccaga
    10921 gcgtgattga ccgcctgaac tggatgcgtg atgtgcaggg accaatactg cgcgacgcga
    10981 tgaaaattat cggcgaaatc gatctgcgct taatgctggc gcaggcgctg catatgggcg
    11041 atgagtgcca taaccgcaat aacgccggga cgacgctgct gattcaggcg ctgacgccgg
    11101 ggattatcca ggcgggctat tccgtcgcgc aacagcgcga agtgtttgag tttgttgcca
    11161 gtagcgatta cttctccggc ccgacgtgga tggcgatgtg taaagcggcg atggatgcgg
    11221 cgcacggcat cgaatacagc accgtcgtta ccaccatggc gcgtaacggc gtcgagttcg
    11281 gcctgcgggt gagcggcctg ccggggcagt ggtttaccgg cccagcgcag caggtcatcg
    11341 gcccgatgtt tgctggttat aagccggaag attcggggct ggatatcggc gacagcgcca
    11401 tcacagaaac ctacggtatt ggcgggtttg ccatggcgac cgcgcctgca atcgtcgcgc
    11461 tggtgggggg cacggtggaa gaggctatcg atttctcccg tcagatgcgc gaaatcaccc
    11521 ttggcgaaaa ccccaacgtc accattccgc tgctcggttt tatgggcgtg ccgtcggcag
    11581 tcgacatcac ccgcgtgggt agcagcggca ttctgccggt gatcaacacc gccatcgccc
    11641 ataaagatgc gggcgtcggc atgattggcg cgggcattgt gcatccacca ttcgcctgct
    11701 tcgagaaagc cattcttggc tggtgcgaac gttacggcgt ttgacctacc tgttcat
//
LOCUS       DAZGXS010000128        11703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGXS010000128 DAZGXS010000000
VERSION     DAZGXS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            132..425
                     /locus_tag="ACHKG5_003812"
                     /pseudo
     CDS             132..425
                     /locus_tag="ACHKG5_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(483..914)
                     /gene="tnpA"
                     /locus_tag="ACHKG5_003813"
     CDS             complement(483..914)
                     /gene="tnpA"
                     /locus_tag="ACHKG5_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFP0869820.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            922..2130
                     /locus_tag="ACHKG5_003814"
     CDS             922..2130
                     /locus_tag="ACHKG5_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0869821.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2174..2281
                     /locus_tag="ACHKG5_003815"
                     /pseudo
     CDS             2174..2281
                     /locus_tag="ACHKG5_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2265..2903)
                     /gene="ytfB"
                     /locus_tag="ACHKG5_003816"
     CDS             complement(2265..2903)
                     /gene="ytfB"
                     /locus_tag="ACHKG5_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0869822.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3121..3741
                     /gene="fklB"
                     /locus_tag="ACHKG5_003817"
     CDS             3121..3741
                     /gene="fklB"
                     /locus_tag="ACHKG5_003817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0869823.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4050..5462
                     /gene="cycA"
                     /locus_tag="ACHKG5_003818"
                     /gene_synonym="dagA"
     CDS             4050..5462
                     /gene="cycA"
                     /locus_tag="ACHKG5_003818"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0869824.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5507..6169)
                     /gene="ytfE"
                     /locus_tag="ACHKG5_003819"
     CDS             complement(5507..6169)
                     /gene="ytfE"
                     /locus_tag="ACHKG5_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0869825.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6277..7242)
                     /gene="ytfF"
                     /locus_tag="ACHKG5_003820"
     CDS             complement(6277..7242)
                     /gene="ytfF"
                     /locus_tag="ACHKG5_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0869826.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7350..8210)
                     /gene="qorB"
                     /locus_tag="ACHKG5_003821"
     CDS             complement(7350..8210)
                     /gene="qorB"
                     /locus_tag="ACHKG5_003821"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0869827.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8299..8679
                     /gene="ytfH"
                     /locus_tag="ACHKG5_003822"
     CDS             8299..8679
                     /gene="ytfH"
                     /locus_tag="ACHKG5_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0869828.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8797..10740)
                     /gene="cpdB"
                     /locus_tag="ACHKG5_003823"
     CDS             complement(8797..10740)
                     /gene="cpdB"
                     /locus_tag="ACHKG5_003823"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0869829.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10930..11670
                     /gene="cysQ"
                     /locus_tag="ACHKG5_003824"
     CDS             10930..11670
                     /gene="cysQ"
                     /locus_tag="ACHKG5_003824"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0869830.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2755 a         2932 c         3058 g         2958 t
ORIGIN      
        1 cattatggat ataacgatgc acccggggag aaaatgactt ccacgcctta caccgtacat
       61 tcttgttgtg cggtatgtaa ttattctacg ctgtcggtat cggtgtattc gttaatcagc
      121 aggttaacca gccacaaaaa gccattgaga aaattattga ttttacatgg gattattata
      181 ttgctaaccc ctggttttta aaaattgtgc atagtgaaaa tcaaagtaaa ggtgtgcatt
      241 atgcaaaatc acagcgtctc ttagaaatca accatgcgca cttgcagtta atggaatcct
      301 tattggatga agggaagaaa tacaatattt ttaagccaga tatcgaccca ttgcaggtga
      361 atattaatat tgctgcgctt gggggatatt atttgatcaa ccagcatacg cttggcctgg
      421 tttatccggt gcgccgtaaa accccgtcct tcagggcggg gatatacggc gcggttttcc
      481 acctaaccag gtgtttgctg ttgttcaatg tattgacgga tggcggatat tggcgcacct
      541 ccgcagctac tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact
      601 gcaatatctg gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc
      661 agactggata tcgccagttt gggaggatag ttaattaaca aatggacgtg atctggttcg
      721 ccatccattt caaccagttc agcttcaaaa tcagcacata cctttgaaaa gtaagtgcgt
      781 agtttttctg tcgcgtcatg gtcaaaaatc tggcgtcggt atctggtgac aaagaccagg
      841 tgaacatgca tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca
      901 gaccaaagta tcatttggcc tgtgaaacga ctacaggcat ttaaattcca gttaagaccc
      961 ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
     1021 cgtgctctgg cgcttcagaa tgagaatcat gaggccggta ataaatacat cccttacggg
     1081 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
     1141 gccccgtcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
     1201 ttccggaagc gggctgcttt tccccgattc aaaaagcggg gacagaatga tgcattccgc
     1261 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaacttggc
     1321 tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc
     1381 cagtcctgcg gtaagtggta catcagtatt cagactgaaa atgaagtatc aactccggtt
     1441 cacccttcag cattaatggt cggactggat gctggcgtgg ctaaactcgc cacgctgtca
     1501 gatggcacag tctttggacc tgtaaacagt tttcagaaaa accagaagac gctggcgaga
     1561 cttcagcgac agttaagccg caaggtcaaa ttcagcaaca actggcagaa gcagaaacgc
     1621 aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct tcacaaagtc
     1681 acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa ggtcagcaac
     1741 atgtcaaagt cagcagcggg tacggtcagc cagccggggc gcaatgtccg ggcaaaatca
     1801 ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgccgcca gcttgagtac
     1861 aagcagctct ggcgtggcgg tcaggtgctt gctgttccgc cagcgtacac aagccagcgt
     1921 tgcgcgtgct gtggtcatac agcgaaagaa aatcgcctgt cacaaagtaa attcagatgc
     1981 caggcatgtg gatatacagc gaacgctgat gtaaatggcg ctcgtaacat tttagcggcg
     2041 gggcacgccg ttcttgcctg tggggagatg gtgcagtcag gccgcccgtt gaagcaggaa
     2101 cccaccgaaa tgattcaggc gacagcctga acgtagcagg gatccacgtc cttcagggct
     2161 tggaggatgt caacacatca gtatggtttc accccaggca ctggaagcca gacgtaaggt
     2221 catcaaagag acaatcctta gctggctttt ggttgaccct tcatctaccg cgcacgaata
     2281 aaactgccat ccggctggcg ggtgaacagg acctgttgat tattccccgt atcaatggtt
     2341 aagcccgtca ccacgccgct ggcgttctgg cggattttca ccatctgccc gttttgcaaa
     2401 ttactcagcg gcttacccgc gccttctacc tgcgccattg catagacatc ggtagcgggc
     2461 agtccgtggt cgcggaacag ctgcgccatc gttttaccgg gttcgacacg gtaagagcgc
     2521 cattgattat caattccgct atctggctgg aatggttgcg tttgggtagt tttcggctgt
     2581 tcttctggct ggccttcctg tataggttct ggagcgaccg gagccacctg atcaggataa
     2641 ttttgtgggg tgactaattg cgcccgcagc tgttcttctg tcggcggttg tgattgcgac
     2701 tgaatatcta actgcgcttc acgcgtcacc accggcgcgt tgggcgtatc atcagatgga
     2761 agcagaaatc cgaccaccaa tacaatggcg gcaataatga tcccccgacg atgcatcggc
     2821 ggcagcgggt ccatgaagcg aaaattgtcc ggtgcgtgcc agactttctc cagggttggt
     2881 tttagttcaa agcgcccggg catggtttcc tcctgctccg cgtcttgttc ctcaatcata
     2941 gcctatgaat aagctaacgc tatgatgtcc gtggtaaacc cgcctttatt atattcatac
     3001 gggatattgc tattgtttct ttttccctgg gatttgtcat cattcccgcg acaaagtttt
     3061 acccaaagaa gtgtggctga tatgctgccc gctactttat accctaagaa aggaaatacg
     3121 atgaccaccc caacttttga caccatcgaa gcgcaagcaa gctacggcat tggtttgcag
     3181 gtagggcaac aactgagtga atctggcctg gaagggctgc tgccagaagc actggttgca
     3241 ggtattgccg atgcgctgga aggcaaacat ccggctgttc cggttgatgt ggtgcatcgc
     3301 gcgctgcgtg aaatccacga gcgcgccgat gccgttcgtc gtcagcgttt ccaggcgatg
     3361 gctgctgaag gtgtgaaata cctggaagaa aacgccaaaa aagaaggtgt gaatagcacc
     3421 gaatctggcc tgcaattccg cgtgatcaac cagggtgaag gcgcaattcc ggcacgtacc
     3481 gaccgcgttc gtgttcatta caccggtaaa ctgatcgacg gcaccgtgtt tgacagctcc
     3541 gttgctcgtg gtgaacccgc tgaatttccg gttaatggcg tgatccctgg ctggattgaa
     3601 gcactgactc tgatgccggt aggttctaaa tgggaactga ctatcccgca ggaactggca
     3661 tatggcgagc gcggcgcagg cgcatccatc cctccgttca gcaccctggt gtttgaagtc
     3721 gaactgctgg aaatcctcta agcagcgcat tctgttcccc tcgaaccaga ggggagcagg
     3781 cattcagcaa taaacccttc agtttgccaa acggcgctat tttgtgttgc aaagaccccg
     3841 taagcgtgta tttttgtgag ctgtttcgcg ttatcaccgt gatatgacac tcactttaaa
     3901 cataaaatta acattagatc taaatcttag tattcatccc gcgtattgtt acctaatatc
     3961 gatgagtccc gatacagatt cgtcgtatca tagactgact aaaggccgta gagcctgaac
     4021 aacacagaca ggtacaggaa gaaaaaaaca tggtagatca ggtaaaagtc gttgccgatg
     4081 atcaggctcc ggctgaacag tcgctacggc gcaatctcac aaaccgacat attcagctta
     4141 ttgccattgg tggtgccatt ggtacggggt tgtttatggg gtctggcaaa acgattagcc
     4201 ttgccgggcc gtcgatcatt ttcgtttata tgatcattgg ttttatgctc tttttcgtga
     4261 tgcgggcaat gggggaattg ctgctttcga atctggaata caaatctttt agtgacttcg
     4321 cttccgattt actcgggccg tgggcaggat atttcaccgg ctggacttac tggttctgct
     4381 gggttgtaac cggtatggca gacgtggtgg cgatcacggc ttatgctcag ttctggttcc
     4441 ccgatctctc cgactgggtc gcctcgctgg cggtgatagt gctgctgctg acgcttaacc
     4501 tcgccactgt gaaaatgttc ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg
     4561 ccattgtgtc gctgattgtc gtcggcctgg tcatggtggc gatgcacttt cagtcaccga
     4621 ctggtgtgga agcgtcattc gcgcatttgt ggaatgacgg cggctggttc ccgaaaggtt
     4681 taagtggctt ctttgccgga ttccagatag cggttttcgc tttcgtgggg attgagctgg
     4741 taggtacaac agctgcggaa accaaagatc cagagaaatc actgccacgc gcgattaact
     4801 ccattccgat ccgtatcatt atgttctacg tcttcgcgct gattgttatt atgtccgtga
     4861 cgccgtggag ttcggtagtc ccggagaaaa gcccgtttgt tgaactgttt gtgttggtag
     4921 gtttgcctgc ggctgccagc gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg
     4981 ctaacagtgg cgtcttctct accagccgta tgctgtttgg tctggcgcag gaaggtgtgg
     5041 caccgaaagc gttcgctaaa ctttctaagc gcgcagtacc cgcgaaaggg ttgactttct
     5101 cgtgtatctg tctgctcggc ggcgtggtga tgttgtatgt gaatcctagc gtgattggcg
     5161 cgttcacgat gattacaacc gtttccgcga ttctgtttat gttcgtctgg acgattatcc
     5221 tttgctcgta ccttgtgtat cgcaaacagc gtcctcatct acatgagaag tcgatctaca
     5281 agatgccgct cggcaagctg atgtgctggg tatgtatggc gttctttgtg ttcgtggtcg
     5341 tgttgctgac actggaagat gacactcgcc aggcgctgct ggttaccccg ctgtggttta
     5401 tcgcgctggg gctgggctgg ttgtttattg gtaagaagcg ggctgctgaa ctgcggaaat
     5461 aaccgcatta tcatgctgga tggcgcaatg ccatccagct tttagatcac tcacccgcca
     5521 gcgcccttgg gaacagtaca ttgttttcca ggctgatgtg atccatcagg tcatcaatca
     5581 gttcattaat gccgttatac atcgctttcc aggtggtgca ggcttccggc ggcggtgtga
     5641 cgttattggt attgtgtttg atcacttcca gcagttcacc cgcttcatcg tgctcgcttt
     5701 ccattacgct gattggcccc attgcctggc tgcccatgcc ttgtttgatc atcgggaaga
     5761 ggatctgctc ttctttcatc atgtggctgg aaagctcttc atgcagcatg gtcaggtatt
     5821 ttgtcagccc ttttggcacg ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt
     5881 gcagaatcag ctccggcagt tgctcgcggt gacgagcgtg gtagcgcacg atgatatggt
     5941 cgatgatttc tgccagcggg gcgctacgcc agtctttctc aatcggttgt tcagcaagct
     6001 ttgccagctc agcttcaatg acctcaacat ccagttcttt acgtgccgcc gcacgcgcca
     6061 gcgtttgctt accgccgcag cagtaatcca tatcatattt acgaaacaga gccgaagcgc
     6121 gaggaataga gagcgccagt tcacctaaag gttggtcgcg ataagccata gctgatacct
     6181 cattcttaat aagataagat gtattttaaa tgcaccttta aggcaaaagc tataaccctt
     6241 acgtagcaga ggcttattta actcactgcc agcgtgtcaa ctttctgaga cagattcagt
     6301 taaaggtttt tctggttttg ctctaaccgc gatgaccacg ccaatcacta acagcgcgat
     6361 accgctcagc gtcattagcg gcggcatttg ctggcggagt aaaaaggtgt acagcaaacc
     6421 tgccagcgtt tcgaaaacaa tcagcggccc gagaatcact gtcggtaatc gctggctggc
     6481 gacgttccag cagagtgcgc caacccatga gcaaagcacg gctatcgcaa tcatcagact
     6541 aataaacacc agcggacggg ggccaaaggg tagggagaag tccggcgttt gcgtattcag
     6601 ccagtaacaa gcgacgagat agccgataag tgaaaccggc agtgtgacca gcgcctgcgc
     6661 tgttgcccac atcatcggat gtttgtcggg attttcccgc agccagcggg cgttgcgcag
     6721 ggcataccac gcccagcaga ccacggaaac taacgccagc acgatgccag aggtataacg
     6781 tgcccagtca aaattgggga gtccgtggtt taactcagca atattcacac acgccaggcc
     6841 gatgccaata caaatcagtg ccggggcgag ttttccccac gcgagtttgc cgtcgcgctg
     6901 gctataaagc agattggcga agacgggaat caccaccggc agggtgccga taatcatcgt
     6961 ggaaacaggc gcaccagtac gttgaatggc actggcaagg cagaagtaat agatgaggtt
     7021 gcccatcata gtgagcatca aggcggtaag ccagtcgcga cgcgccaact gacgcagacg
     7081 cacgcgtccc agccaggcaa tgggcagcgc aattaaccct aacgccagat aacgccccat
     7141 cgactgcaac atcgccgggt attccggcac gatcaacggc ccgacaaaaa taagccccca
     7201 catcaaccct gctaacaggg cgtacagcac gccgctaatc attactggca tccattgatc
     7261 tgtcagaaga aagttcagga agcagacatg attgctcccg gcatcccgtc attatcagag
     7321 aggatgcggg aggatgccac tttaattaac tagttattaa cattaaaaag atggcttacg
     7381 ctttcggcta acgttgtcgt tgggtggcca atcaatttgc taagtgtttt gctgtcatca
     7441 aacagaccgc ctttcgatgc gccaacgtca gaatccgcca gcatatccgc cagtccgtcg
     7501 ggcagtccga cgcttttcag tgccgcggcg aaatcggctt cgctcagatt ttgataggta
     7561 acctgtttgc cgctctgttt ggtcagctct gccgctaact gtgtcaacgt ccaggcacta
     7621 tcgcccgcca gttcgtaaac cttgccttcg tgaccggctt cgctaatcac gcgtgccgca
     7681 gctgccgcat aatctgcccg cgttgctgag gcgattttgc catcgcccgc cgcaccgata
     7741 aatacgccgt gttccagtgc tgccggggcg ctggcgaggt agttttcggt gtaccagccg
     7801 ttgcgcagta gggtgtaaac gatgccagaa tcagccagca ttttctccgt ctcgatgtgc
     7861 tcatcggcga ggccgagcgg agatttatcc gcgtgaagca ggctggtata agcgataaat
     7921 ttcacgccag ccgtctttgc ggcattaata acattacgat gctgcggggc acgttgaccc
     7981 acttcgctgg aagagatcag cagtagtttt tccactccct gaagtgcaga tgtcagtgcg
     8041 gcttcatcgc cgtagtcagc ctgacgcacg gtaatgcctt gtgctgtcag tgcctgggct
     8101 tttgccggat tacgaacgat agccactatt tggctggcag gaaccgtttt catcaaggat
     8161 tcaataacat actgaccaag ttggccagtg gcaccagtaa tagcgatcat gggaagtctc
     8221 catcgttttg tcttgtgttt aaatacgcta acacctaaac ttacttttag taagtacgca
     8281 caaaaaggta agtatgaaat gagtcaggtt agcctgtcgc aacaactgaa agagggtaat
     8341 ctctttgcgg aacagtgccc gtcgcgcgag gtgttgaaac acgtcaccag ccgttggggg
     8401 gtgttgattc tggtggcgct acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa
     8461 atcggcgggg tgagcgaaaa gatgcttgcg cagtcgttac aggcgttaga acaggatggt
     8521 tttcttaacc gtatcgcgta tccggtggtg ccgccgcatg ttgaatacag cctcacgccg
     8581 ctgggcgagc aggtgagcga aaaggttgct gcactggcgg actggattga gttgaatttg
     8641 cccgaggtgt tggcggtgcg ggatgaacgt gcggcataac tgccggatgc gcggcctacg
     8701 gtgtatgact tgtagggcgg ataagatgcg ccagcatcgc atccgccaat aaatgccgga
     8761 tgcgattgat gatttatagg ccgaaaaaga agtgttttac ttactcaaat ccacctgata
     8821 aatcgcaaac ccgatatcat cggtcgcgac tttattcatc ggatactgcc ctttctcttt
     8881 aataaacgct gcggctttat ctgacgggga agtttcgaaa cggatatcca gtttcttatc
     8941 ggcagctatc ggtgctaaac gccagttgtt atctgccgcc gggtgaattt cccccgcacg
     9001 tttcgactca tcagcaatcc acgctgccag caccgagcgg ttctcatccg gtgaagcaaa
     9061 agcgatatgg ctgtcgcccg tcccggcaaa tttgccgccg taagcgcgat agttattggt
     9121 ggcaacgagg aacatagcgt tcggatcaat cggcttgcca ttaaaggtca ggttcttaat
     9181 cctttccgca ttggcattaa tcatctggca ctcgccgtca taacgggcgg gctgggtaac
     9241 atcaatctga taattcacac catcaataac atcaaagtta taagtgcgga aaccatccca
     9301 gttgatgagt gactgtggtt tcgtgctgtt gggatcaatc tggttaaact gtccggcgga
     9361 gcactccagc cactctttca cctctttacc gctggctttc accacaatca gcgtattggg
     9421 atagagataa agatcggcgg cattacggaa ggtcaactgg cctttttcca cctccacata
     9481 gcttgccggg tcatttttgc gaccaccgac tttaaacggt gcggcagctg aaagcaccgg
     9541 cagttttgcc agatccggat cgccctgaat gtaatgctcg acatacgctt tttgcgcgtt
     9601 gttcactact tgcacggtcg gatcgtcctg caccagcgcc agatagctat acatattgtc
     9661 ggcagattta ccgattggct tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag
     9721 tgtttctacc agcttgctgt cttccgccgc gagggatttt ttattggcga tgtcgtaaat
     9781 cggccgtgct tccgctttcg cctgcgtcac ctgccattta ccgctgtcat tactgagttg
     9841 taagtcgacc accccaagat gatcgcccca catgcccggc attaccgccg gaacaccatt
     9901 cagcgtgcct ttggtgatat cagccccttc gatatcagca aaatctttac ccgggaaaac
     9961 ggcgtgagca tggccaaaca taatggcgtt aacgcccgga atttcactga ggtaataaac
    10021 tgagttttcc gccattactt tatacggatc ggcagacagc ccggaatgcg ccagaacgac
    10081 aacgacatcg gcacctttct cgcgcatttc aggctcgtat ttgcgcacgg tttcggtaat
    10141 atcattcacc gtcacttttc cggataaatt agctttatcc cagcccatga tttgcggcgg
    10201 cacgacgcca atatagccaa tcttcagcgt ctgttttttt ccgtctttat cgaccacttc
    10261 ggtgtcttta attaaatacg gtgtaaacat tggctgtttg gttctggcgt caatgacgtt
    10321 ggcatttaca taagggaatt tcgctcctgc cagcgcattt ttcaggtaat ccagaccgta
    10381 gttaaactcg tggttgccaa gcgttccgac ggtatagtcc agcgtattta atgccttata
    10441 gaccgggtga atatcacctg cttttaatcc tttcgccgac atgtaatcgg ccagcggact
    10501 cccctgaatc aaatcgccgt tatcgaccag tacgctgttt ttcacttcat tgcgggcatt
    10561 gttaatcagg cttgccgtac gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata
    10621 atcgaaatcc atcatgttgc tatgcagatc agtggtttcc atgatacgca gatcgaccgt
    10681 cgctgcattc acactggcgg caatcagcgt ggccaggagc gttgcgctaa acttaatcat
    10741 cagggacatc cttttatcat cgggaatacg aaagaaaagg gagaataaac gtcttactta
    10801 tagaacagtg aagaatgcca caattttacg ctttgaaaat gatgacacta tcacagttgg
    10861 cgcattcatt aatgataggg tataagtaaa acaataagtt aacaccgctc acagagacga
    10921 ggtggagaaa tgttagatca agtatgccag cttgcacgga atgcaggcga tgccattatg
    10981 caggtctacg acggggcgaa accgatggac gtcgtcagca aagcggacaa ttctccggta
    11041 acggcagcgg atattgccgc tcacaccgtt atcatggacg gtttacgtac gctgacaccg
    11101 gatattccgg tcctttctga agaagatcct cccggttggg aagtccgtca gcactggcag
    11161 cgttactggc tggtagaccc gctggatggt acgaaagagt ttattaaacg taatggcgaa
    11221 ttcaccgtta acattgcgct cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg
    11281 ccggtaatga acgtaatgta cagcgcggca gaaggcaagg cctggaaaga agagtgcggc
    11341 gtgcgcaagc agattcaggt ccgcgatgcg cgcccgccgc tggtggtgat cagccgctct
    11401 catgcggatg cggagctgaa agagtatctg caacagcttg gcgaacatca gaccacgtcc
    11461 atcggctctt cgctgaagtt ctgcctggtg gcggaaggac aggcgcagct gtacccgcgt
    11521 ttcggaccaa cgaatatttg ggacaccgcc gctggacatg ctgtggctgc agctgccgga
    11581 gcgcacgttc acgactggca gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg
    11641 aatccggggt tcagagtgtc tatttactaa attcagatgg cagaaacagt gtatttcctg
    11701 att
//
LOCUS       DAZGXS010000129        11664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGXS010000129 DAZGXS010000000
VERSION     DAZGXS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..238
                     /locus_tag="ACHKG5_003825"
     CDS             <1..238
                     /locus_tag="ACHKG5_003825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869831.1"
                     /translation="RSAAFHQDFRKIRNHASEFTDLIILILIYFEKGIGIYAVFLKIL
                     IIGMDFRARKGFYMDTLLIISSSHHQQFTSLFRV"
     gene            331..2754
                     /gene="pgaA"
                     /locus_tag="ACHKG5_003826"
     CDS             331..2754
                     /gene="pgaA"
                     /locus_tag="ACHKG5_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP0869832.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2763..4781
                     /gene="pgaB"
                     /locus_tag="ACHKG5_003827"
     CDS             2763..4781
                     /gene="pgaB"
                     /locus_tag="ACHKG5_003827"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP0869833.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4774..6099
                     /gene="pgaC"
                     /locus_tag="ACHKG5_003828"
     CDS             4774..6099
                     /gene="pgaC"
                     /locus_tag="ACHKG5_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP0869834.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6101..6514
                     /gene="pgaD"
                     /locus_tag="ACHKG5_003829"
     CDS             6101..6514
                     /gene="pgaD"
                     /locus_tag="ACHKG5_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP0869835.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6564..7352)
                     /gene="phoH"
                     /locus_tag="ACHKG5_003830"
     CDS             complement(6564..7352)
                     /gene="phoH"
                     /locus_tag="ACHKG5_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0869836.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7972..9243)
                     /gene="efeB"
                     /locus_tag="ACHKG5_003831"
     CDS             complement(7972..9243)
                     /gene="efeB"
                     /locus_tag="ACHKG5_003831"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0869837.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9249..10376)
                     /gene="efeO"
                     /locus_tag="ACHKG5_003832"
     CDS             complement(9249..10376)
                     /gene="efeO"
                     /locus_tag="ACHKG5_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0869838.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10434..11264)
                     /gene="efeU"
                     /locus_tag="ACHKG5_003833"
     CDS             complement(10434..11264)
                     /gene="efeU"
                     /locus_tag="ACHKG5_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0869839.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3074 a         2858 c         2731 g         3001 t
ORIGIN      
        1 ccgtagcgca gcctttcatc aggactttcg aaaaatccga aatcatgcat cggaatttac
       61 tgatttaatt attttaatcc taatttattt tgaaaaaggc atcgggattt atgccgtatt
      121 cctgaagatc ctcatcattg gaatggattt tcgggcgaga aaaggatttt atatggacac
      181 tctgctcatc atttcttctt ctcatcatca acaattcacg tctctcttcc gcgtttaata
      241 acggattatg aggtgcaaaa atatctttct tttcagttac ctgtaattag atacagagag
      301 agattttggc aatacatgga gtaatacagg atgtattcaa gtagcagaaa aaggtgcccg
      361 aaaaccaaat gggctttgaa acttcttact gccgcatttt tagcagcgag tcccgcggcg
      421 aagagtgctg ttaataacgc ctatgatgca ttgattattg aagctcgcaa gggtaatact
      481 cagccagctt tgtcatggtt tgcactaaaa tcagcactca gcaataacca aattgctgac
      541 tggttacaga ttgccttatg ggccgggcaa gataaacagg ttattaccgt ttacaaccgc
      601 taccgtcatc agcaattacc agcgcgtggt tatgcagctg tcgccgtcgc ttatcgtaac
      661 ctgcaacaat ggcaaaactc gcttacactg tggcaaaagg cgctctctct ggagccgcaa
      721 aataaggatt atcaacgggg acaaatttta accctggcag atgctggtca ctatgatact
      781 gcgctggtta aacttaagca gcttaactct ggagcaccgg acaaagccaa tttactcgca
      841 gaagcctata tctataaact ggcggggcgt catcaggatg aattacgggc gatgacagag
      901 tcattacctg aaaatgcatc tacgcaacaa tatcccacag aatacgtgca ggcattacgt
      961 aataatcaac ttgctgccgc gattgacgat gccaatttaa cgccagatat tcgcgctgat
     1021 attcatgccg aactggtcag actgtcgttt atgcctacgc gcagtgaaag tgaacgttat
     1081 gccattgccg atcgcgccct cgcccaatac gctgcattag aaattctgtg gcacgataac
     1141 ccagaccgta ctgcccagta ccagcgtatt caggttgatc atcttggcgc gttattaact
     1201 cgcgatcgtt ataaagacgt tatttctcac tatcagcgat taaaaaagac ggggcaaatt
     1261 attccgccct gggggcaata ttgggttgca tcggcttatc tcaaagatca tcagccgaaa
     1321 aaagcacagt caataatgac cgagctcttt tatcacaagg agaccattgc cccggattta
     1381 tccgatgaag aacttgcgga tctcttttac agccacctgg agagtgaaaa ttatccgggc
     1441 gcgctaactg tcacccaaca taccattaat acttcgccgc ctttccttcg gttaatgggc
     1501 acgcctacga gcatcccgaa tgatacctgg ttacaggggc attcgtttct ctcaaccgta
     1561 gcaaaatata gtaatgatct tcctcaggct gaaatgacag ccagagagct tgcttataac
     1621 gcaccaggaa atcagggact gcgcattgat tacgcgagtg tgttacaagc ccgcggttgg
     1681 cctcgtgcag cagaaaatga attaaaaaaa gcagaagtga tcgagccacg taatattaat
     1741 ctggaggttg aacaagcctg gacagcatta acgttacaag aatggcagca ggcagctgtc
     1801 ttaacgcacg atgttgtcga acgtgaaccg caagatcccg gcgttgtacg attaaaacgt
     1861 gcggttgatg tacataatct tgcagagctt cgtatcgctg gctcaacagg aattgatgcc
     1921 gaaggcccgg atagtggtaa acatgatgtc gacttaacca ccatcgttta ttcaccaccg
     1981 ctgaaggata actggcgcgg ttttgctgga ttcggttatg ccgatggaca atttagcgaa
     2041 ggaaaaggga ttgttcgcga ctggcttgcg ggtgttgagt ggcggtcacg taatatctgg
     2101 ctcgaggcag agtacgctga acgcgttttc aatcatgagc ataaacccgg cgcgcgcctg
     2161 tctggctggt atgattttaa tgataactgg cgtattggtt cgcaactgga acgcctctct
     2221 caccgcgttc cattacgggc aatgaaaaat ggtgttacag gcaacagtgc tcaggcttat
     2281 gttcgctggt atcaaaatga gcggcgtaag tacggtgtct cctgggcttt cactgatttt
     2341 tccgacagta accagcgtca tgaagtctca cttgagggtc aggaacgcat ctggtcttca
     2401 ccatatttga ttgtcgattt cctacccagt ctgtattacg aacaaaatac agaacacgat
     2461 accccatact acaaccctat aaaaacgttc gatattgttc cggcatttga ggcaagccat
     2521 ttgttatggc gaagctatga aaatagctgg gagcaaatat tcagcgcagg tgttggtgcc
     2581 tcctggcaaa aacattatgg cacggatgtc gtcacccaac tcggctacgg gcaacgcatt
     2641 agctggaatg acgtgattga tgctggcgca acgctacgct gggaaaaacg accttatgac
     2701 ggtgacagag aacacaactt atacgttgaa ttcgatatga cattcagatt ttaaggataa
     2761 atatgttacg taatggaaat aaatatctcc tgatgctggt gagtataatt atgctcaccg
     2821 cgtgcattag ccagtcaaga acatcattta taccgccaca ggatcgcaaa tctttactcg
     2881 ccgagcaacc gtggccgcat aatggttttg tagcgatttc atggcataac gttgaagacg
     2941 aagctgccga ccagcgtttt atgtcagtgc ggacatcagc actgcgtgaa caatttgcct
     3001 ggctgcgcga gaacggttat caaccggtca gtattgctca aattcgtgaa gcacatcgag
     3061 gaggaaaacc gctaccggaa aaagctgtag tgctgacttt tgatgacggc taccagagtt
     3121 tttatacccg cgtcttccca attcttcagg ccttccagtg gcctgctgta tgggcccccg
     3181 tcggcagttg ggtcgatacg ccagcggata aacaagtaaa atttggcgat gagttggtcg
     3241 atcgagaata ttttgccacg tggcaacaag tgcgagaagt tgcgcgttcc cggctcgttg
     3301 agctcgcttc tcatacatgg aattctcact acggtattca ggctaatgcc accggcagct
     3361 tattgcctgt atatgtaaat cgtgcatatt ttactgacca cgcacggtat gaaaccgcag
     3421 cagaataccg ggaaagaatt cgtctggatg ctgtaaaaat gacggaatac ctgcgtacaa
     3481 aggttgaggt aaatccacac gtttttattt ggccttatgg cgaagcgaat ggcatagcga
     3541 tagaggaatt aaaaaaactc ggttatgaca tgttcttcac ccttgaatca ggtttggcaa
     3601 atgcgtcgca attggattcc attccgcggg tattaatcgc caataatccc tcattaaaag
     3661 agtttgccca gcaaattatt accgtacagg aaaaatcacc acaacggata atgcatatcg
     3721 atcttgatta cgtttatgac gaaaacctcc agcaaatgga tcgcaatatt gatgtgctaa
     3781 ttcagcgggt gaaagatatg caaatatcaa ccgtgtattt gcaggcattt gctgatcccg
     3841 atggtgatgg gctggtcaaa gaggtctggt ttccaaatcg tttgctacca atgaaagcag
     3901 atatttttag tcgggttgcc tggcaattac gtacccgctc aggtgtaaac atctatgcgt
     3961 ggatgccggt attaagctgg gatttagatc ccacattaac gcgagtaaaa tacttaccaa
     4021 caggggagaa aaaagcacaa attcatcctg aacaatatca ccgtctctct cctttcgatg
     4081 acagagtcag agcacaagtt ggcatgttat atgaagatct tgccggacat gctgcttttg
     4141 atggcatatt gttccacgat gatgctttgc tttcagatta tgaagatgcc agtgcaccgg
     4201 ctatcacggc ttatcagcaa gcaggcttta gcgggagtct gagcgaaatt cgacaaaacc
     4261 cggagcaatt taaacagtgg gcccgcttta aaagtcgtgc gttaactgac ttcactttag
     4321 aacttagtgc gcgcgtaaaa gccattcgcg gtccacatat taaaactgca cgaaatattt
     4381 ttgcacttcc ggtaatacaa cctgaaagtg aagcctggtt tgcacagaat tatgctgatt
     4441 tcctaaaaag ctatgactgg accgctatta tggctatgcc ttatctggaa ggtgtcgcag
     4501 aaaaatcggc tgaccaatgg ttaatacaat tgaccaatca aattaaaaac atccctcagg
     4561 ctaaagacaa atctatttta gaattacagg cacaaaactg gcagaaaaat ggtcagcatc
     4621 aggctatttc ttcgcaacaa ctcgctcact ggatgagcct attacaactg aatggagtga
     4681 aaaactatgg ttattatccc gacaattttc tgcataacca acctgaaata gaccttattc
     4741 gtcctgagtt ttcaacagcc tggtatccga aaaatgatta atcgcatcgt atcgtttttt
     4801 atattatgtc tggtgttatg cataccccta tgcgtagcgt actttcactc tggtgaactg
     4861 atgatgaggt tcgttttctt ctggccgttt tttatgtcca ttatgtggat tgttggcggc
     4921 gtctatttct gggtctatcg tgaacgccgc tggccgtggg gagaaaacgc accagctccc
     4981 cagttgaaag ataatccgtc tatctccatt atcattccct gttttaatga ggagaaaaac
     5041 gttgaggaaa ccatacacgc cgctttagca cagcgttatg agaacattga agttattgcc
     5101 gtaaatgacg gttcaacaga taaaacccgt gccatcctgg atcgcatggc tgcacaaatt
     5161 ccccatttgc gggtcattca tctggcgcaa aaccagggga aagccattgc gcttaaaacc
     5221 ggagctgccg cggcgaaaag tgaatatctg gtgtgcattg atggcgatgc gttattagac
     5281 cgcgatgcgg cggcatatat tgtggaaccg atgttgtaca acccgcgtgt gggtgccgta
     5341 accggtaatc ctcgtattcg aacacgttct accctggtgg gtaaaattca ggttggcgag
     5401 tattcctcaa ttattggttt gatcaagcga acccagcgta tctatggaaa cgtatttacc
     5461 gtttccggtg ttattgccgc atttcgtcgc agcgccctgg cagaagtggg ttactggagt
     5521 gacgatatga tcaccgaaga tattgatatt agctggaagc tgcagttgaa tcagtggacg
     5581 attttttacg agccacgggc actgtgctgg atattaatgc ctgaaacgtt aaaagggctg
     5641 tggaaacagc gcctgcgctg ggctcagggc ggtgcagaag tattcctcaa aaatatgaca
     5701 aggttgtggc gcaaagaaaa ctttcgaatg tggccgctgt tttttgaata cagcctgacg
     5761 acaatatggg ccttcacctg cctggtcggt ttcattattt acgcagtcca acttgccggt
     5821 gtaccgttaa atattgaatt gacacatatc gctgcgacac atactgccgg aatattattg
     5881 tgtacgttat gtttactgca atttattgtc agcctgatga tcgagaatcg ctatgagcat
     5941 aatctgactt catcgctttt ctggattatt tggttcccgg ttattttctg gatgctgagc
     6001 ctggcaacga cattggtatc atttacacga gtcatgttga tgcctaaaaa gcaacgcgcc
     6061 cgttgggtaa gtcccgatcg cgggattctg agaggttaat atgaacaatt taattattac
     6121 gacccgacaa tcaccagtac gtttactggt tgattatgtt gccacaacca tcttgtggac
     6181 attatttgcg ttgttcatat tcttattcgc catggatctg ctgacgggtt attactggca
     6241 aagcgaggcc agaagccgac ttcagttcta ttttttgctg gcagtggcga atgccgtcgt
     6301 gttaattgtc tgggcgctgt acaataagct gcgttttcaa aaacagcagc atcatgcagc
     6361 ctaccaatat acgccgcaag aatatgcaga gagcttagca atacctgatg agctctatca
     6421 gcaactacaa aaaagccaca ggatgagcgt acacttcacc agccaggggc aaataaaaat
     6481 ggttgtttca gaaaaagcgc tagtcagggc ataaacaccc aaaacaaagc ccggttcgcc
     6541 cgggctctgc accgataata cacttaactg tatgcatgca gcgtacgttg gcaaagtgcc
     6601 gaacgtacgc aatcctcttt tccgaaccgg acgatcccaa ccatttcatc ttcttcgaaa
     6661 cgttccagcg cgtcacttaa tccggagcac acgccgcgag gcaaatcgca ttgcgtgata
     6721 tcaccgttga cgataaccgt cacgttctcc ccgaggcggg ttaaaaacat tttcatttgc
     6781 gcggcagtca cattctgcgc ctcgtcaaga atgacgactg cattttcaaa ggtacgtcca
     6841 cgcatatagg cgaacggcgc aatttccacc ttccctattt ccggtcgcag gcagtactgc
     6901 ataaaggaag cccctaagcg ccggaccagc acgtcgtaga ccgggcgaaa atagggagca
     6961 aacttttctg cgatatctcc aggtaagaag ccaagatctt catcggcttg cagaactgga
     7021 cgggtgacga taatcctgtc gacatcctta tgtatcaggg cctctgccgc ttttgctgcg
     7081 ctgatccagg tttttccgca cccggcttcg cccgtggcga atatcagctg cttactctca
     7141 atagccttca gatagtgcaa ttgcgcttca tttcgcgcga ggatgggcga agtatcgcga
     7201 ctgtcgcggg ccataccaat ggcttctacg ccgcccatct gcacaagcga ggtgaccgat
     7261 tcttcttcac gctgcttatg gctgcgcgaa tcccgtctca gcacacgttt tgcctcgcga
     7321 cgagctttga tcactgcttt ttgtcttccc atggagagca ccttgagttg tttgtattca
     7381 tcacacgcgc cgttggcagc gcgattatgc gcacgaacat cagagggttg gcttccttgt
     7441 aagccatagt ttgcttttgg ataaaatgcc gaaaaacggc tacgcgcacc gtttacggcg
     7501 tcggtaacac atgaaaagaa aggatgaggt tgaaaatgca aagtgacgag atgactaccg
     7561 gaggagaaaa ctccgcgagt ggtggcgcgt tgattatcta aaacatgtcc agtacaggac
     7621 gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact
     7681 taataagtac aacagttctc gcatttattg caacaatata tttacttata tttaactata
     7741 aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg
     7801 aaaaaagctg tgtgctttca gcaggatttg catcaggcat cagtgcacga tcgccgtatg
     7861 cggcgtgaac gccttatccg cctaccgaac ccgcaccgat tgtaggcata agaagacgcg
     7921 caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctaaaaac attaaacccg
     7981 caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata
     8041 accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac
     8101 tgtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag
     8161 ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga
     8221 ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcccctt
     8281 cccttccggg tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc
     8341 accggtttgc ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca
     8401 aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc
     8461 aggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct
     8521 atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt
     8581 actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc
     8641 cggcgtatgt ttgatgatat cgcgcagtgc atggatcacc gtgtcctggg tgttggcgca
     8701 aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt tgggaaaacg
     8761 cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa
     8821 tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat
     8881 gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt
     8941 cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg ctttatcgct
     9001 ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc
     9061 ctgatgctca ccataaaacg gctgtttctc attgcgtgca accggtgaaa gcgtacccgg
     9121 cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc
     9181 aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat cttcatactg
     9241 cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca
     9301 gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa
     9361 ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact
     9421 tttgccaaca gttccgggtt agctttttgc agttgtggac gcagcagatc gacaattttc
     9481 tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct
     9541 tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc gccgaccact
     9601 tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa
     9661 agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa tgctttttcc
     9721 agacggtgga aaccggtgaa ttttggatca gcggcttttt gctcgtaatc atcttcacgg
     9781 gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag
     9841 tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg
     9901 tcggtaaagg ctttggtgtc ggtcaccagc tgtgtggttt ccgccatgac atacgcttta
     9961 tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt
    10021 gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg
    10081 tattcgccag gctgtaaatt cgccgtcatt ttctggctaa agccaggggc gatattttcc
    10141 cgctcttcca ccaccatcac gcctttgagg atctcccatt ccagcgcctt ctggctgtgg
    10201 ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc
    10261 ttatccgtca cggtcacttt gacctgcggc acatcagcgg cgttagccat aaaagcagaa
    10321 gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg
    10381 agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg
    10441 gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg
    10501 agataaataa accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaatc
    10561 ccttccatca gcgtgccaaa cagcgagtga gttgagagca ccgcactcat atcgaaggcg
    10621 atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc accagctgcc
    10681 agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg
    10741 aggcgaatac cgccccagta gagcaggaag cctagcacca cggcagtagc aagaccgagc
    10801 attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag
    10861 accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca
    10921 tgatgatttc cacgctgcaa tgcgctatcg acagcctgct ccagttgtac tttgacgttg
    10981 cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc
    11041 acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg
    11101 aagatcccca agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca
    11161 atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg
    11221 gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa
    11281 cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta
    11341 cgctaaagaa aaatacaagc ggaatattta taaaatgatg ttggaatgta aaaatgagga
    11401 gatcaaatcc ggagaaaaaa gcgattattt taaggtattt agctgacgtt tcgcccggca
    11461 gatgaagctt gtacgaatgt tggtggcgac aaactcgctg cagcgtgccc gaagggttga
    11521 attttcagtt agtcgtttca caaaagaacg ttgttttttg cttattaata gatagtgata
    11581 aggttgtgaa tgaaattata cctcatctcc tcatcgaatt ttttgcccac aacctaaaca
    11641 ggagaaaacg tgtatttaag tctg
//
LOCUS       DAZGXS010000130        11638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGXS010000130 DAZGXS010000000
VERSION     DAZGXS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..253
                     /locus_tag="ACHKG5_003834"
     CDS             <1..253
                     /locus_tag="ACHKG5_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0869840.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="ACHKG5_003835"
     CDS             265..843
                     /locus_tag="ACHKG5_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0869841.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSVSQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="ACHKG5_003836"
     CDS             840..1235
                     /locus_tag="ACHKG5_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0869842.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="ACHKG5_003837"
     CDS             1243..1983
                     /locus_tag="ACHKG5_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0869843.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="ACHKG5_003838"
     CDS             1999..2421
                     /locus_tag="ACHKG5_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0869844.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="ACHKG5_003839"
     CDS             2403..2837
                     /locus_tag="ACHKG5_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0869845.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="ACHKG5_003840"
     CDS             2830..5379
                     /locus_tag="ACHKG5_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0869846.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="ACHKG5_003841"
     CDS             5376..5705
                     /locus_tag="ACHKG5_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0869847.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..6403
                     /locus_tag="ACHKG5_003842"
     CDS             5705..6403
                     /locus_tag="ACHKG5_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0869848.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            6409..7152
                     /locus_tag="ACHKG5_003843"
     CDS             6409..7152
                     /locus_tag="ACHKG5_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0869849.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            7149..7721
                     /locus_tag="ACHKG5_003844"
     CDS             7149..7721
                     /locus_tag="ACHKG5_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0869850.1"
                     /translation="MKTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGI
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7782..11180
                     /locus_tag="ACHKG5_003845"
     CDS             7782..11180
                     /locus_tag="ACHKG5_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0869851.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQDNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            11247..>11638
                     /locus_tag="ACHKG5_003846"
     CDS             11247..>11638
                     /locus_tag="ACHKG5_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0869852.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGD"
BASE COUNT         2664 a         2901 c         3716 g         2357 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggtgtca caggttgccc gtgagacaag ggtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcctg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac agtgacgccg atccggagca ggaggtgatc agccgctggc gcattgagca
     6121 gtgcagcgaa ctgagcgcgg tgagtgcctc ttttgtactg tccacgccga cggaaacgga
     6181 cggcgctgtt tttccgggac gtatcatgct ggccaacacc tgcacctgga cctatcgcgg
     6241 cgatgagtgc ggttatcacg gtccggcggt cgcggatgaa tatgaccagc caacgtccga
     6301 tatcacgaag gataaatgca gcaaatgcct gagcggttgt aagttccgca ataacgtcgg
     6361 caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat gacacagaca
     6421 gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg cggcttcgtg
     6481 gtaagcacgc cggaggggga aagatatttc ccctgcgtga atatctccgg tgagccggag
     6541 gcgtatttcc gtatgtcgcc ggaagactgg ctgcaggcag aaatgcaggg tgagattgtg
     6601 gcgctggtcc acagccaccc cggtggtctg ccctggctga gtgaggccga ccggcggctg
     6661 caggtgcaga gtgatttgcc gtggtggctg gtctgccggg ggacgattca taagttccgc
     6721 tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg ttacacgctg
     6781 ttccgggatg cttatcatct ggcggggatt gagatgccgg attttcatcg cgaggatgac
     6841 tggtggcgtc acggtcagaa tctctatctg gataatctgg aggccacagg gctgtatcag
     6901 gtgccgttgt catcagcaca accgggcgat gtgctgctgt gctgttttgg ttcatcggtg
     6961 ccgaatcatg ccgccattta ctgtggtgat ggcgagctgc tgcaccatat tcctgaacaa
     7021 ctgagcaaac gagagaggta taccgacaaa tggcagcgac gcacacactc cctctggcgt
     7081 caccgggcat ggcgcgcatc tgcctttacg gggatttgca acgatttggc cgccgcatcg
     7141 accttcgtgt gaaaacgggg gctgaagcca tccgcgcact ggccacacag ctcccggtgt
     7201 ttcgtcagaa gctgagcgac ggctggtatc aggtacggat taccgggcgg gacgtcagca
     7261 cgtccgggtt aacggcgcag ttacatgaga ctctgcctga tggcgctgtg attcatattg
     7321 ttcccagagt cgccggggcc aagtcaggtg gcgtattcca gattgtcctg ggggctgccg
     7381 ccattgccgg atcattcttt accgccggag ccacccttgc agcatggggg gcagccattg
     7441 gggccggtgg tataaccggc atcctgtttt ctctcggtgc cagtatggtg ctcggtggtg
     7501 tggcgcagat gctggcaccg aaagccagaa ctccccgtac acagacaacg gataacggca
     7561 aacagaacac ctatttctcc tcactggata acatggttgc ccagggcaat gttctgccgg
     7621 ttctgtacgg tgaaatgcgc gtggggtcac gcgtggtttc tcaagagatc agcacggcag
     7681 acgaagggga cggtggtcag gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga
     7741 aaccgcctcc gggcggtttt atcgtttatg gagcatgacg aatgggtaaa ggcagcagta
     7801 aggggcatac cccgcgcgaa gcgaaggaca acctgaagtc cacgcagctg ctgagtgtga
     7861 tcgatgccat cagcgaaggg ccggttgaag gtccggtgga tggattaaaa agcgtgctgc
     7921 tgaacagtac gccggtgctg gacagtgagg ggaataccaa tatatccggc gtcacggtgg
     7981 tgttccgggc cggtgagcag gagcagacac cgccggaggg atttgaatcc tccggctccg
     8041 agacggtgct cggtacagaa gtgaaatatg acacgccgat cacccggacc atcacgtcgg
     8101 caaacattga ccgtctgcgt tttactttcg gcgtgcaggc actggtggaa accacctcaa
     8161 agggggacag gaatccatcg gaagtccgcc tgctggttca gatacaacgt aacggtggct
     8221 gggtgacgga aaaagacatc accattaagg gtaaaaccac ttcacagtat ctggcctcgg
     8281 tggtggtgga taacctgccg ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg
     8341 acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcatacacc gaaattatcg
     8401 atgtgaaaca gtgctacccg aacacggcac tggtcggcgt gcaggtggac tcggagcagt
     8461 tcggcagcca gaaggtgagc cgtaattatc atctgcgcgg gcgtattctg caggtgccgt
     8521 cgaattataa cccgcagacg cggcaataca gcggtatctg ggacggaacg tttaaaccgg
     8581 catacagcaa caacatggcc tggtgtctgt gggatatgct gacccatccg cgctacggca
     8641 tggggaaacg tcttggtgcg gcggatgtgg ataaatgggc gctgtatgtc atcggccagt
     8701 actgcgacca gtcagtgccg gacggctttg gcggcacgga gccgcgcatc acctgtaatg
     8761 cctacctgac cacacagcgt aaggcgtggg atgttctcag cgatttctgc tcggcgatgc
     8821 gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga ccgtcggata
     8881 aggtgtggac ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg tcgttccgct
     8941 acagcttcag cgccctcaag gaccgccata atgccgttga ggtgaactgg attgacccgg
     9001 ataacggctg ggagacggcg acagagcttg tggaggacac gcaggccatt ctccgttacg
     9061 gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag ccgggggcag gcacaccgcg
     9121 ccgggctgtg gctgattaaa acggagctgc tggagacgca gaccgtggat ttcagcgtgg
     9181 gcgcagaagg gcttcgccat gtaccgggcg atgtcattga aatctgtgat gatgactatg
     9241 cggggatcag catcggtggg cgtgtgctgg cggtgaacag ccagacccgg acgctgacgc
     9301 tcgaccgtga aatcacgctg ccatcctccg gtaccacgct gataagcctg gttgacggaa
     9361 gtggcaatcc ggtcagcgtg gaggtccagt ccgtcaccga cggcgtgaag gtaaaagtga
     9421 gccgtgttcc tgacggcgtt gccggataca gcgtatgggg gctgaagttg ccgacgttgc
     9481 gccagcgcct gttccgctgc gtgagtatcc gtgagaacga cgacggcacg tatgccatca
     9541 ccgccgtgca gcatgtaccc gaaaaagaag ccatcgtgga taacggggcg cactttgacg
     9601 gcgacctgag cggcacggtg aatggcgtca cgccgcccgc ggtgcagcac ctgactgccg
     9661 aagtcaccgc agacagcggg gaatatcagg tgctggcgcg ctgggacacg ccgaaggtgg
     9721 tgaagggggt gagcttcctg cttcgcctga ccgtggcagc ggacgatggc agtgagcggc
     9781 tggtcagtac ggccaggacg acggaaacca cataccgctt cacgcaactg gcgctgggga
     9841 actacaggct gactgtccgg gcggtaaatg cgtggggaca gcagggcgat ccggcatcgg
     9901 tatcgttccg gattgccgca ccggcagcgc cgtctcggat tgagctgaca ccaggctatt
     9961 ttcagataac cgccacgccg catcttgcgg tttatgatcc gacggtacag tttgagttct
    10021 ggttctcgga aacgcggatt gcggatatca ggcaggttga aaccagcgcg cgttatcttg
    10081 gtacggcgct gtactggata gccgccagta tcaatatcaa accgggccat gattattact
    10141 tttatatccg cagtgtgaac accgttggca aatcggcatt cgtggaggcc gtcggtcggg
    10201 cgagcgatga tgcggaaggt tatctggatt ttttcaaagg caagataacc gaatctcatc
    10261 ttggtaaaga gctactggaa aaagttgacc tgacggagga taacgccagc agactggatg
    10321 agttttcgaa agagtggaag gatgctaacg ataagtggaa tgccatgtgg ggcgtcaaaa
    10381 ttgagcagac caaagacggc aaacattatg tcgcgggtat tggcctcagc atggaggaca
    10441 cggaggaagg caagctgagc cagtttctgg ttgccgccaa tcgtatcgcg tttattgacc
    10501 cggcaaacgg gaatgaaacg ccgatgtttg tggcgcagga caaccagata ttcatgaacg
    10561 acgtgttcct gaagcgcctg acggccccca ccattaccag cggtggaaat ccaccggcat
    10621 tttccctgac accggacgga aagctgactg ctaaaaatgc ggatatcagt ggcagtgtga
    10681 atgcgaactc cgggacgctc aacaacgtca cgattaatga gaactgtcag attaagggga
    10741 aactgtcagc caatcagatt gaaggcgata ttgtcaaaac ggtcagcaag tctttccccc
    10801 gcacgagcac ttatgccagt ggcaccatca cggtaagaat cagtgatgat cagaaatttg
    10861 accggcaggt catgataccg ccagtgttat tccgcggtgg taagcatgag aatttcaaca
    10921 gtaataacca acagtcatac tggtattcaa cctgccggtt aagagtgacc cgcaatggcc
    10981 aggagatttt taatcagtcc acgacggatg ctcagggcgt attttcctca gttatagata
    11041 tgcctgccgg acaggggacg ctgacactga cattcaccgt atcttcatca ggagcgaata
    11101 actggacacc aacaaccagt atcagcgatc tgctggttgt ggtgatgaaa aaatccacag
    11161 caggtatcag tatcagctga attttataac ccagaacggg cgtcagaaat gacgcctttt
    11221 ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgccgccatt ttgtccgcag
    11281 ccatttgtct ggccgtatcc ggtgcgcctg catgggcatc tgaacatcag tccacgctga
    11341 gcgcggggta tcttcatgcc cggaccaacg ttcccggcag tgatgatctg aacgggatta
    11401 acgtgaaata ccgttatgag tttacggata cgctggggct ggtgacgtca ttcagctatg
    11461 caggagacaa gaatcgccag ctgacccgtt acagcgatac ccgctggcat gaagattccg
    11521 tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca
    11581 gcgcgtatgc gatggcgggt atggcttaca gccgtgtttc gactttttcc ggggatta
//
LOCUS       DAZGXS010000131        11515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGXS010000131 DAZGXS010000000
VERSION     DAZGXS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            347..1213
                     /gene="folD"
                     /locus_tag="ACHKG5_003847"
     CDS             347..1213
                     /gene="folD"
                     /locus_tag="ACHKG5_003847"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0869853.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1215..1427
                     /gene="ybcJ"
                     /locus_tag="ACHKG5_003848"
     CDS             1215..1427
                     /gene="ybcJ"
                     /locus_tag="ACHKG5_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0869854.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1535..2056
                     /locus_tag="ACHKG5_003849"
     CDS             1535..2056
                     /locus_tag="ACHKG5_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0869855.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2092..3477)
                     /gene="cysS"
                     /locus_tag="ACHKG5_003850"
     CDS             complement(2092..3477)
                     /gene="cysS"
                     /locus_tag="ACHKG5_003850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0869856.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3651..4145
                     /gene="ppiB"
                     /locus_tag="ACHKG5_003851"
     CDS             3651..4145
                     /gene="ppiB"
                     /locus_tag="ACHKG5_003851"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0869857.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4148..4870
                     /gene="lpxH"
                     /locus_tag="ACHKG5_003852"
     CDS             4148..4870
                     /gene="lpxH"
                     /locus_tag="ACHKG5_003852"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0869858.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4988..5497
                     /gene="purE"
                     /locus_tag="ACHKG5_003853"
     CDS             4988..5497
                     /gene="purE"
                     /locus_tag="ACHKG5_003853"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0869859.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5494..6561
                     /gene="purK"
                     /locus_tag="ACHKG5_003854"
     CDS             5494..6561
                     /gene="purK"
                     /locus_tag="ACHKG5_003854"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0869860.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6700..7593)
                     /locus_tag="ACHKG5_003855"
     CDS             complement(6700..7593)
                     /locus_tag="ACHKG5_003855"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0869861.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7590..8405)
                     /locus_tag="ACHKG5_003856"
     CDS             complement(7590..8405)
                     /locus_tag="ACHKG5_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0869862.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8416..9675)
                     /locus_tag="ACHKG5_003857"
     CDS             complement(8416..9675)
                     /locus_tag="ACHKG5_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0869863.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9685..11352)
                     /locus_tag="ACHKG5_003858"
     CDS             complement(9685..11352)
                     /locus_tag="ACHKG5_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0869864.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2592 a         3267 c         3021 g         2635 t
ORIGIN      
        1 aaaatagaga gccccgttag atgaatactt ccgcgcaaaa tatattcaac acaaatatag
       61 acctgaagcg gtaaattacc aggctgaaaa ttctttttat attgtcaggt atttcttaaa
      121 ttatcttaat ccttaaacaa ggaaataaat cagttccaga tttacaacgc catcatggac
      181 gaaaaatgaa gctttcaatc tcagcgacct cagcgacagt gcgcctaacc ttcgcaagag
      241 gtcgcttcac gcgataaatc tgaaacgaaa cctgacagcg cgccccgctt ctgacaaaat
      301 aggcgcatcc ccttcgacct acgtaacaga tggaatcctc tctctgatgg cagcaaagat
      361 tattgacggt aaaacgattg cgcagcaggt gcgctctgaa gttgctcaaa aagttcaggc
      421 gcgtattgca gccggactgc gggcaccagg actggccgtt gtgctggtgg gtagtaaccc
      481 tgcatcgcaa atttatgtcg caagcaaacg taaggcttgt gaagaagtcg ggttcgtctc
      541 ccgctcttat gacctcccgg aaaccaccag cgaagcggag ctgctggagc ttatcgatgc
      601 gctgaatgcc gacaacacca tcgatggcat tctggttcaa ctgccgttac cggcgggtat
      661 tgataacgtc aaagtgctgg aacgtataca cccggacaaa gacgtagacg gtttccatcc
      721 ttataacgtc ggtcgtctgt gccagcgcgc gccgcgtctg cgtccctgca ccccgcgcgg
      781 tattgtcacg ctgcttgagc gttacaacat tgataccttc ggcctcaacg ccgtggtgat
      841 tggcgcatcg aatatcgttg gccgcccgat gagcatggaa ctgttgctcg caggttgcac
      901 caccaccgtg actcaccgtt tcactaaaaa tctgcgtcac cacgtagaaa atgccgatct
      961 gttgatcgtt gccgtcggca agccgggctt tattcctggt gactggatca aagaaggcgc
     1021 aattgtgatt gatgtcggca tcaaccgtct ggaaaatggc aaagttgtgg gcgacgtcgt
     1081 gtttgaagac gcggctaaac gcgcctcata cattacgcct gttccgggcg gcgttggccc
     1141 gatgacggtt gccacgctga ttgaaaacac gctacaggcg tgcgttgaat atcatgatcc
     1201 acagggtgag taacatggcg acattttctt taggtaaaca tccgcacgtt gagctgtgcg
     1261 acttgctgaa actggaaggc tggagcgaaa gcggcgcgca ggcgaaaatc gcgattgccg
     1321 aaggccaggt gaaagtcgac ggcgcggttg aaacgcgcaa acgctgcaaa atcgtcgccg
     1381 ggcagacagt gagttttgca ggtcacagcg tacaggttgt tgcctgatcc ctttcccggc
     1441 ctgtatcaac aggccggttc tcttttcaga cttttgccct ctacgccccg ttcatccatg
     1501 ctgatagcat cgacattttg ccgaagcgac catcatgcca accgttatta cgcacgccgc
     1561 cgttccccta tgcattggtt tagggttggg ttcgaaagtc atccctccgc gcctgttatt
     1621 tgccggaatc atcctggcga tgctgcccga tgccgacgtc ttgtcgttta attttggcgt
     1681 tgcttacggc aatgtttttg gtcatcgcgg gtttacccat tcgctggtgt ttgcgtttgt
     1741 tgtcccgctg ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     1801 ctggctgttt ttaaccgtct cgttgctatc gcacagcttg ctggattcgg taaccactgg
     1861 cggtaaaggg gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     1921 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     1981 gattatttct gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     2041 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caatagcgca attacttacg
     2101 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcat taagacggtc
     2161 acgcgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     2221 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     2281 ttccggttct tgctccagca ggcccaatac ggcggaaagt ttacgcaggt gagatgccat
     2341 tgcattcgcc gctgccatat cttctacttt cagacggttt acttcacgcg ccatatcaaa
     2401 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     2461 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     2521 gagacgctcc agcgccgcac gcgcctgctt caggttctct tcgctatagt tcagctggct
     2581 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     2641 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     2701 catccccgag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     2761 gatttcgttt tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     2821 gttacccagc tgcttgcagt tcattgccga acattcaatg tgccagccag gacgacccgc
     2881 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     2941 cattgggttg cgtttgtcgt cgaccacgtc aacgcgcgcg cctgcctgca gctggtcgag
     3001 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     3061 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     3121 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca acgcatcaaa
     3181 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     3241 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagctt
     3301 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     3361 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     3421 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     3481 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     3541 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     3601 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     3661 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     3721 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     3781 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     3841 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     3901 caatggcccg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     3961 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     4021 ctgaagtggt tgaaggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     4081 gcggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     4141 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     4201 caccgccggt tttctgcgtt ttttagcggg agaagcccgc aaggccgacg cgctgtatat
     4261 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     4321 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     4381 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agtggcatga cgttattgcc
     4441 ggaagaaaaa gtgctcgaac tttatggtcg tcgggtgctg attatgcatg gcgacacgct
     4501 gtgcaccgat gacgcgggtt atcaggcttt tcgcaccaaa gtccacaaac cctggctgca
     4561 aacgctattc ctcgctctgc cgttgtttgt gcgcaaacgc attgccgtgc gaatgcgcgc
     4621 gaacagtaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     4681 ggtagtcagt gcgatggaaa aacatcaggt gcaatggctg atccacgggc atacccatcg
     4741 cccggcggtg catgaactta tcgccaatca gcaacctgct tttcgcgtgg tactgggtgc
     4801 ctggcatacg gaaggttcga tggtgaaagt cacggcggat gacgttgagc tgattcattt
     4861 tccgttttaa aaaacccgca actttgctga tttcacagcc acgcaaccgt tttccttgct
     4921 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     4981 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     5041 cgactgggct accatgcagt tcgccgccga aatcttcgaa atcctgaatg tcccgcacca
     5101 cgttgaagtg gtttctgctc accgcacccc cgataaacta ttcagcttcg ccgaaagtgc
     5161 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     5221 catgattgcc gccaaaacgc tggtgccagt gctgggcgtg ccagtacaga gcgccgcact
     5281 gagcggtgtc gatagcctct actccatcgt acaaatgcca cgcggcattc cggtgggtac
     5341 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattcttgc
     5401 gactcatgat aaagaactgc accagcgtct gaatgactgg cgcaaagcac aaactgatga
     5461 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     5521 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     5581 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     5641 atcgaacgct ggccggaaac agcattaacc cgcgagctgg cgcgccatcc ggcctttgtg
     5701 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaagcagct tttcgataag
     5761 ctccacctgc cgactgcacc gtggcagtta cttgccgaac gcagcgagtg gcctgcggtg
     5821 tttgagcgtt taggtgagct ggcgattgtt aagcgtcgca ctggtggcta tgacggacgc
     5881 ggtcaatggc gtttacgcgc cgatgaaacc gagcagttac cggctgaatg ctacggcgaa
     5941 tgtattgtcg agcagggcat aaacttctct ggtgaagtgt cgctggttgg cgcgcgctgc
     6001 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     6061 cgcaccagcg tcgcttttcc gcaggccaat gcgcagcagc aagcgcaagc cgaagagatg
     6121 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     6181 accccgcaag gtctgctgat caacgaactg gcaccgcgtg tgcataacag cggtcactgg
     6241 acacaaaacg gtgccagcat cagccagttt gagctacatc tgcgggcgat taccgatctg
     6301 ccgttaccgc aaccagtggt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     6361 gtgaattatg actggctgaa actgccgctg gtgcatctgc actggtacga caaagaagtc
     6421 cgtccgggcc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgtcc
     6481 gcgacgctgg aagccttgat cccgctgctg ccgccggaat atgccagcgg cgtgatgtgg
     6541 gcgcagagta agttctgtta acctggtgct catttgattg ccggatgcgg cgtgaacgcc
     6601 ttatccggcc tacaccgatc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg
     6661 catcaggcat ggtgcgccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc
     6721 cgcttcgccc gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttacc
     6781 acggcttctg acataaccac tcaccgccgt taccttcggc cccatcgaac catcggcttt
     6841 ggcaaatggc gctaactcat ccggtgtggc atggcgaatg gcacgttgct gcggcattcc
     6901 ccagttttca tataccgcat cagcatcggt gaggatcacc agtccatcgg cgttaatttg
     6961 ctcggcgagc aacgcagcgg cgagatcttt atcaatcact gcttcactcc ctgctccgtc
     7021 atccgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
     7081 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
     7141 caaatattta ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg cctcttgttc
     7201 ttctggctga taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg
     7261 cgaaacttca atgcgcgtcc gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa
     7321 actctgcgcc agcatatagc caatcatccc ctgactttct gccaccagca catccagcgg
     7381 atacggttct acctctttcc acgccagatt ctgcaatgcc agcagcccca cctgcggccc
     7441 gttgccgtga acaatcgcca gccgataaga acgggccagg cgcgccagcg cgggtacagc
     7501 actggcgata ttgcgatatt gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc
     7561 gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa ttgttggcca
     7621 agccagaacc ccagcagtgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca
     7681 atcgcaacag cggtacgttt cgggcaactc agagcatgaa caaagtgcag gagtggcgag
     7741 gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt
     7801 tgcagattgt cggaacaggc aaaaaacgga cgaccggagc gcgcatctaa agcaccataa
     7861 taccaggccg ccagcagcat tccgctcagc gtgtcatcat gacttggcgt taatccgggg
     7921 cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
     7981 tgacgaaact ggcgtaactc agcaggcagc ggatcgcttg ccgccaacgc cagtggcccg
     8041 aaaagcccgg tttcctccgc gcgttgcatc catgcagctg caagtggttg aggatgcgca
     8101 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg
     8161 cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
     8221 tcgaattgcg cacggcgaag cacccatcct ccggggccga aaccactacc ctgacgatgc
     8281 agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca cactcccgct
     8341 aaccgccagg actggcgata attcggtgcg ctactactgg caagcagagg atggatgatc
     8401 gtcattacgc gttcctcaac caatccccat gctttcagcc agtgcttcca gcgcctgttc
     8461 aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc ccgcctcttt
     8521 atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc gacgaatgtc
     8581 cagtcccagg cacgcgccct gaaaatccca gcttgggatc tgcaactgca tattgcgttc
     8641 aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc cactcgcacc
     8701 gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac caaaggtttc
     8761 ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa
     8821 cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg
     8881 aatcccgaac atattgccgt tgcgggtcat tgccgtgacg atgctgcctg cgcggatcat
     8941 cgcgccagca tccatcgccg ccttgcagta agccatcgcg aggttgagga agaactgatc
     9001 ggtcacgctg aggaaatcca tcacttccgc gatgtgctgt ttatcatgat cgaggcgagc
     9061 aatttgtggg gccagcgcac gcattaacag tgctgaggaa gcaatattgc gttgatggaa
     9121 ctcatcgccc atcgtaatgc cctgcgccat catcgcagta agatcgatac cgcgctccat
     9181 gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc gatggcgagt
     9241 caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt cgttgaggtt
     9301 gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca tactggcaga
     9361 agtaataccg cccattggcc cgacggcatt cacatggtga caaggaatga agttcacttc
     9421 cccctgctcc agtattgcca gcgcctgcgc ttcatctttc gcccaacctt cgaacagaca
     9481 tgcgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc
     9541 ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaagaag ccggttgcac
     9601 atccagccag tgtggacgag cgcgacgaat ttgttcgata accgcagcat tggcttgcgc
     9661 cactgatgta aacatgagaa ccccttattg caaacgttct aataaacgag ccagtttttt
     9721 attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg cgctttgtag
     9781 ctccagcgca aaactgcgta atccgatgtt aatcacggcg acgttttcca gtaatgacgg
     9841 tgtgtgttgc tgtgcggcag gcgagagcgg atgaattaac gctgccgcca gcaaggtggc
     9901 ttccggtagc gaactcacga ccgcaatccc cgcatcttcc agcgtggcga tttgctgcga
     9961 gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca gtggttgatt
    10021 gtctgaacgc gcagcacagg ctttttgcca ggcgcttacc agcgaggcgg caggatcggc
    10081 ggtcgcaccg aagccaatca cgacatcaag cagcagcaca cgcacttgcg gtttagcgcc
    10141 gagatcggta attaactggt tgcgtaaggt tgggtcgatc atcggatggg gacgcccgac
    10201 ggtgtagaaa tcatcgccga ggtcaataat ctggtggcca tcggcgtcca gcatcatgcc
    10261 gtgatgatgg gcatcatcgg cttccacgcc aaggtgtccg gcaagtaatc ccgccgcttc
    10321 ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctgg caggcattat
    10381 cgcgttacgt cgcgccgtaa cgcgtgaaag caggcaagcc aggcgtgcgg cctcatccag
    10441 cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag
    10501 cgccaccgtc ggtttgccgg ttgctttcat ggcattaaca attttcagac gcaccgcttc
    10561 ggcaggtggt tttgaaacaa atgccagcac ttcgcttttc tcgtctgcac tgagcatttc
    10621 cagcgccgtc agcgcactga tgccgcccac ttcacggctg aggtcgcggc cgccaaggcc
    10681 aatcgcgtga gtaattccct cccctgccag cgcaatctgc gagcacagct cctgaatccc
    10741 ggtaccggaa gcgccaatga cgccaatgtt gccttccggc atcacgttag caaaagccag
    10801 cggcgtgccg gcaatcatcg acgtaccgca gtccggcccc atcaccagca agcctttttc
    10861 ccgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg acgttatcgg agaacatcat
    10921 cacgttgagg ttgcgatcca gcgcctgatt cgccagctcc gccgcatact cgccagccac
    10981 tgaaatcagc gccagattgg catcgggtaa tttctgacag gcactgtccc agcgacgcac
    11041 ctgcgtcaac gcctggctgc tgcctgaccc ctgcgccagt tgttttagcg cctcttcaag
    11101 ctgctgcata atcgcctgcg cgatccccgc atccgccgct tcgctacgaa ttgccacgca
    11161 aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag aaacctgtgg tatctaataa
    11221 cgctttattg gcgggcgtac ccatcattac ggaaacatca tcaacatttt ctgattcgct
    11281 gagttttcgt gaaataatca ttaaactgac cgaatcctga aaacaccctt ttttaataaa
    11341 ggcgtggatc ataacaactc cttaaaaatt gcacccatgg caattgcacg agcagtgtta
    11401 atgtggcgat tacggtatcg atgacaacga gtaaataagg tgatgaatat cacacaggga
    11461 ttattaacaa aagaattcaa ataaccgcca tgctattacg tgttattttt taata
//
LOCUS       DAZGXS010000132        11477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGXS010000132 DAZGXS010000000
VERSION     DAZGXS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..78
                     /locus_tag="ACHKG5_003859"
     CDS             <1..78
                     /locus_tag="ACHKG5_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869865.1"
                     /translation="NEAIREEPIDFLDRSFSSSSWPFYH"
     gene            384..1772
                     /gene="narU"
                     /locus_tag="ACHKG5_003860"
     CDS             384..1772
                     /gene="narU"
                     /locus_tag="ACHKG5_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0869866.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1854..5594
                     /gene="narZ"
                     /locus_tag="ACHKG5_003861"
     CDS             1854..5594
                     /gene="narZ"
                     /locus_tag="ACHKG5_003861"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0869867.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5591..7135
                     /gene="narH"
                     /locus_tag="ACHKG5_003862"
     CDS             5591..7135
                     /gene="narH"
                     /locus_tag="ACHKG5_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0869868.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7135..7830
                     /gene="narW"
                     /locus_tag="ACHKG5_003863"
     CDS             7135..7830
                     /gene="narW"
                     /locus_tag="ACHKG5_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0869869.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7827..8507
                     /gene="narI"
                     /locus_tag="ACHKG5_003864"
     CDS             7827..8507
                     /gene="narI"
                     /locus_tag="ACHKG5_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0869870.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8586..9479
                     /gene="yddE"
                     /locus_tag="ACHKG5_003865"
     CDS             8586..9479
                     /gene="yddE"
                     /locus_tag="ACHKG5_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0869871.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9575..10419)
                     /gene="nhoA"
                     /locus_tag="ACHKG5_003866"
                     /pseudo
     CDS             complement(9575..10419)
                     /gene="nhoA"
                     /locus_tag="ACHKG5_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10718..11161
                     /locus_tag="ACHKG5_003867"
     CDS             10718..11161
                     /locus_tag="ACHKG5_003867"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0869872.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11165..11392)
                     /gene="pptA"
                     /locus_tag="ACHKG5_003868"
     CDS             complement(11165..11392)
                     /gene="pptA"
                     /locus_tag="ACHKG5_003868"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP0869873.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2708 a         2975 c         3022 g         2772 t
ORIGIN      
        1 aatgaggcaa taagagagga accgatagac tttctggaca gatcgttttc ctccagctca
       61 tggccttttt atcactaatt ctataagtgt ctggtcttgt taaaaatata gttaatatac
      121 aaccctttgg attcatcatc tgaagggttg aatcatgaca tgcgtacata gcttaacccc
      181 gtttaatggt tagcttgtta gttacattta gtaacacata actggctgct taatccccat
      241 tctgcccccg cggttacgct ttcttatcta ttctttcaat cagcacttca cccatttcat
      301 ccttaattct aataactggc tattgcgcct tacctcctgt tttgtctttt tttcattacc
      361 aatgtgtaca tgtgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt
      421 tacgcgactg gaaaccagaa aatccggcct tctggaaaaa taaaggaaaa catattgctc
      481 gaagaaacct ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat
      541 ttagcgcagt taccgttaat ctcaataaaa tcggttttaa tttcactacc gatcaactct
      601 ttttattaac cgcattaccc tccgtttctg gcgcattatt acgtgtcccc tactccttta
      661 tggtgcctat attcggtgga cgtcgatgga cggtttttag tactgcaatc ctgattattc
      721 cttgcgtctg gctcggaatt gccgtgcaga atccgaatac tccttttggg atatttatcg
      781 ttatcgcttt gctatgcggt tttgcaggtg cgaactttgc ttcgagcatg ggcaatatca
      841 gtttcttctt tccaaaagcc aggcaaggta gcgctcttgg gattaatggc ggattaggaa
      901 acttaggtgt aagtgtaatg cagctggttg caccgctggt catttttgta cccgtatttg
      961 cctttctcgg cgtcaatgga gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg
     1021 ccgcatggat ttgggtaccg ctactagcga ttgccacgat cgccgcctgg tcagggatga
     1081 atgatatcgc cagttcacgc gcgtcaattg ccgaccagct ccctgtctta caacgcctgc
     1141 atctctggct gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg
     1201 cgggtttcgc catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt
     1261 tctttggccc atttatcggt gccatcgcgc ggtcggtggg tggtgctatt tccgataaat
     1321 tcggcggcgt gcgggtgacg ttgatcaact ttatttttat ggcgattttc agcgccctgc
     1381 tgttccttac cttaccgggc acaggctccg gtaacttcat cgccttttac gccgtattta
     1441 tggggctgtt tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca
     1501 tctttcgcca gataaccatt tatcggatga agatgaaagg cggtagtgat gagcaagctc
     1561 agagagaagc cgtcaccgaa acggcggcgg ctctgggctt tatctcagcc atcggcgcag
     1621 tgggcggctt ttttattccg caggcatttg gcatgtcact caatatgacc ggctctccag
     1681 tgggcgcgat gaaagtgttt ttaatcttct acatcgtttg tgtgctactg acctggctgg
     1741 tttatggtcg gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccgtgt
     1801 cgcatccgac aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta
     1861 aacttttgga tcgctttcgc tacttcaaac aaaagggcga tacctttgcc gatggtcacg
     1921 gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg
     1981 acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc tggaaaatct
     2041 acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc
     2101 ctgacctgcc caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc
     2161 tttacagcgc taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc
     2221 gcgaagccct caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc
     2281 cgcaaaagag cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact
     2341 ggccggaact aaaccagctg attgccgccg ctaacgtctg gactatcaaa acctacggcc
     2401 cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg
     2461 gaacacgtta tctgtcacta ttaggtggca cctgtttaag cttctacgac tggtattgcg
     2521 acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg
     2581 actggtataa ctccagttac atcatcgcgt gggggtctaa cgtaccgcag acacgtacgc
     2641 cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc
     2701 ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccaaaa caaggcactg
     2761 atagcgccct ggcgatggca atgggccatg tgattttaaa agagtttcat ctcgataatc
     2821 ccagcgacta ctttatcaac tactgccgcc gctatagcga catgccgatg ctggtaatgc
     2881 tggaacctcg cgacgatggt agctacgtac ccgggcgcat gatccgcgcg tctgacctgg
     2941 tagatggact aggcgaaagc aataatccgc agtggaaaac cgtggcggtt aataccgcag
     3001 gtgaattggt agtgccgaat ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga
     3061 atctggaatc cattgccgcc ggtaaggaaa ccgaattgtc gttaaccctg ctcggtcaac
     3121 atgacgccgt tgcaggcgtg gctttcccct actttggcgg catcgaaaat ccgcattttc
     3181 gcagcgtcaa acacaatcct gtcctggtgc gtcaattacc cgttaaaaac ctgacattag
     3241 ccggtggcag cacctgtcca gtggtcagcg tttatgattt ggtactggcg aattacggcc
     3301 tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca
     3361 ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa accatcgctc
     3421 gtgaatttgc cgatactgcc cataaaacgc atgggcgctc gatgattatc ctcggcgcag
     3481 gtgttaacca ctggtatcac atggacatga actaccgggg aatgatcaat atgctgatct
     3541 tctgcggttg tgtcggacaa agcggtggcg gctgggcaca ctatgtcggt caggaaaaac
     3601 tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac cgaccaccgc
     3661 gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag
     3721 tctctgcgca ggagttactt tcaccgcttg ccgatgccag taagtacagc ggtcatctga
     3781 ttgattttaa cgttcgcgcc gaacgcatgg gctggctacc ttctgcgccg cagttggggc
     3841 gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta
     3901 ccgcccaggc gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca
     3961 gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc tccggcaaag
     4021 gccacgagta tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac
     4081 tcggtgccag cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag
     4141 gcaagctcga cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg
     4201 atatcgttct gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc
     4261 atccatttat tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact
     4321 gggaaatcta caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca
     4381 aagaaaccga cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc
     4441 cgtgtgaagt gctcgactgg cgcaaaggcg aatgcgatct gagcccgggt aaaaccgcgc
     4501 cgaatattgt ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg
     4561 ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat acgcaggatg
     4621 aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccagcg caggggcgtc
     4681 cgctgattga caccgccatt gacgcttcag aagtgattct ggcactggca ccagaaacca
     4741 acggtcatgt tgcagttaaa gcgtggcagg cgctgggcga gatcaccgga cgcgaacata
     4801 cccatctggc gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc
     4861 cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac gtctcctata
     4921 atgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc
     4981 agctctatca ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcatatcgcc
     5041 cgcctatcga cacccgtagc gtcagtgaga tgcgccagat cccgccaaac ggcttcccgg
     5101 aaaaagcact taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg
     5161 aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaaccg
     5221 atgcccgtga actaaccatt gtcgataacg actgggtgga agtgtttaac gccaatggcg
     5281 cgctgacggc ccgcgcggtg gtcagccaac gtgtaccgcc gggtatgacc atgatgtatc
     5341 acgctcagga acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggta
     5401 ttcataactc ggtcacccgc gtttgcccga aaccaacgca tatgattggc ggttacgcgc
     5461 agctggcctg gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca
     5521 tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg
     5581 aggcgaaaaa atgaaaatcc gttcgcaagt cggcatggtg cttaatctcg ataaatgtat
     5641 cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg aaggcatgga
     5701 gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga
     5761 agatcaggaa gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tacgcccgcg
     5821 tctgggcagc aagatgggtg taataaccaa aatcttcgcc aacccggtgg tgccgcagat
     5881 tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg caccggaagg
     5941 caaacatatc cctactgctc gcccgcgttc gctgattgac ggtaagcgga tggacaaagt
     6001 gatctggggg ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga
     6061 ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat
     6121 gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag
     6181 cggcgctatc tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg
     6241 tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag
     6301 cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtccggtc aaccgaccgt
     6361 gtgctcagaa acctgcgtgg gtcgcatccg atatctgggc gtgctgcttt acgacgctga
     6421 ccgcatcgag gaagcggcga gcaccgagcg cgaggttgac ctctatgaac gccagtgcga
     6481 agtgttcctc gatccgcacg atccctcagt gatcgaggaa gccctgaaac aaggtattcc
     6541 acaaaatgtg attgacgctg cccagcgttc gccagtctac aaaatggcga tggactggaa
     6601 actggcacta ccgctgcacc ctgaataccg caccctgccg atggtctggt acgttcctcc
     6661 gctgtcaccg attcagtcct acgcagatgc gggcggtttg ccgaaaagcg aaggcgtgct
     6721 gcccgccatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt tgagtgccgg
     6781 cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc actatatgcg
     6841 ttcacaaacc gtggaaggcg ttactgatac tcgtgccatc gacgaagtag gcctgagcat
     6901 cgcccaggtc gaagaaatgt atcgctatct cgccattgcc aactatgaag atcgttttgt
     6961 tatcccgacc agccatcggg aaatggcggg cgatgccttc gcagaacgca atggctgcgg
     7021 ttttaccttt ggtgacggtt gtcacggctc ggacagcaaa ttcaacctgt tcaacagtag
     7081 ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg aataatgcag
     7141 atcctcaaag tgatcggcct gttgatggag tatccggacg agatgttgtg ggaatgcaag
     7201 gaggacgcgc tggcgttgat ccgccgcgat gcgccgatgc ttacggattt cacccgcaac
     7261 ctgcttaacg cgccgctgct ggataaacag gccgaatggt gcgaagtgtt tgaccgcggg
     7321 cgcaccacgt cgctgctgct gttcgaacat gttcatgccg agtcccgcga tcgcggccag
     7381 gcaatggtgg acctgctggc ggagtatgaa aaggtcggcc tgcagctgga ttgtcgggaa
     7441 ctgcccgatt atctaccgct gtatctggag tatttaagcg tactgcctga cgatcaggcg
     7501 aaagaaggat tgctcaacgt tgcgccgatc ctcgccctgc ttggcggtcg cttaaaacaa
     7561 cgcgaggcac cgtggtacgc gttgtttgat gctctgctgc aactggcggg aagtacactt
     7621 tcaagtgaca gtgtcacaaa acaagtaaac agcgaagagc gcgatgacac ccgccaggcg
     7681 cttgatgcag tgtgggaaga ggaacaggtg aagtttattg aagataacgc cacggcgtgt
     7741 gacagctcgc cgcttaatca atatcagcga cgctttagtc aggatgtcgc gccgcaatat
     7801 gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt ttttacgata
     7861 tctacccgta catttgtgcg acggtgtttt tcctcggcag ctggctgcgc tacgactacg
     7921 ggcagtacac ctggcgcgcc tcctcaagtc agatgctcga taaacgcggg atggtgatat
     7981 ggtcgaatct gttccatatc ggcattttgg ggatcttctt cgggcacctg ttcggcatgt
     8041 taacgccaca ctggatgtac gcgtggtttt tgccagtggc agcgaaacaa ctaatggcga
     8101 tggtgctcgg tggtatttgc ggtgtgttga cgttgattgg cggcgcagga ctgctgtggc
     8161 gcaggctgac aaatcagcgc gtgcgtgcca cttccactac gccggatatc atcatcatga
     8221 gcattttgct gatccagtgt ctgttggggc taagtaccat accgttctca gcacagtatc
     8281 ctgatggtag cgaaatgatg aagctggtgg gttgggcgca aagcattgtc actttccgtg
     8341 gtggatcgtc agagatgctt aacggtgtag cgttcgtctt ccgcctgcat ctggtgttgg
     8401 gaatgaccat cttcttgctc ttcccattca cccgactggt gcatgtgtgg agcgccccgt
     8461 ttgagtactt tactcgtcgg tatcagattg tgcgctcgcg gcgataattt caccctggcc
     8521 ggatagccct tcgctctccg gccacttgca ggctatgata gttctccagt cacaggagaa
     8581 cgttaatgaa accgcaggtt taccacgtcg atgcttttac ctctcaacca tttcgcggca
     8641 attctgccgg tgttgttttc cccgccgata atcttagcga agcgcaaatg cagcttatcg
     8701 cccgcgaatt aggtcattcg gaaaccgctt ttctgctgca cagcgacgac agcgatgtgc
     8761 gcatccgcta ctttacgcca acagttgaag tgcctatttg cggtcacgcg acagtagctg
     8821 cgcactatgt acgtgcgaag gtgttaggtt taggaaattg cacggtctgg caaacatcgc
     8881 tggcaggaaa acatcgcgtg actatcgaaa agcaccacga tggttatcgt atttcgctgg
     8941 aacaaggtac gccaggcttt gagccaccgc tggaaggtga aacacgtgcg gcgattatca
     9001 acgcgcttca tctcactgag gacgatattc tgccaggctt gccaattcag gtggcaacca
     9061 caggacactc aaaagtgatg atcccactga aaccggaagt ggatatcgac gccctttcgc
     9121 ctgaccttaa tgcgctgacc gctatcagca aacagattgg ctgcaatggt ttcttcccgt
     9181 tccagatccg cccaggcaaa aacgaaaccg atggtcgcat gttctcgcct gcgattggta
     9241 ttgtggaaga tccggtgact ggcaatgcca atggcccgat gggtgcatgg ttggtacatc
     9301 acaacgtatt gccccacgat ggcaacgtgt tgcgtgttaa aggccatcag gggcgcgcat
     9361 tggggcgtga cggtatgatt gaagtgacgg tgacaattcg tgataaccaa ccggaaaaag
     9421 tcaccatttc tggcgcggcg gtgattttgt ttcatgccga atgggcaatt aaactctgaa
     9481 accgttgcct gatgcgcttc gcctgtcagg tctacaaccg ggctaatgta tgccggataa
     9541 ggcgtttacg ccgcatccgg caacctgaca taaattattt tcccgcctcc gggtgcgtat
     9601 caaacgccgc catcaccagc gctaactcat ccacggtaaa gccatgtttc gcatcatcca
     9661 cgcccagacc aaactgttct tgcatcacag catataacga cgccacatcc ggtagatttc
     9721 gctgctccac cgcgtgccca ttttcataat gggaaaatga aaattggtca gtgtcagctt
     9781 gccgccgtcc ggcaaatggc ggcacatcag caaatgatgg cgaaaatgcg actgcggcca
     9841 gtgcgccgac cagaaattac ccatcacata atcgctttgt tgctgctcgc agagatcaaa
     9901 acggtacatc gactgccagt gctgatgatg attaaactgc aacacccaat catcaccctc
     9961 ctgcaacaac cgatactctc cgtgtggcgt ggtctgcaca agatcgggaa ctaaacgaat
    10021 cggcgcggtt agcgtctgcc caccgaaacc gacatcagca atccattttt cctcttccag
    10081 ttccaccaac agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg ataacactac
    10141 ccgccctaac aagctgcgaa cgttaaaccc cagctcgcgt aacacccgct caaaaacgcc
    10201 attctgctca aaacagtaac cgccacgacg ggctatcacc agtttctctt ccagcgattg
    10261 atcatcaagc tgcatttccc tcggcagcaa aacgtcgagg ttttcaaacg gaatggtgca
    10321 attgtgtttc aggtgcaatg cacgaagcgt atcaatattg accgcagcag ctcccgacca
    10381 gttaatacgg gcaaaatagt gattcagaat gggcgtcatc aggcgtttcc cttagtggtt
    10441 ttctcaactt ataacctgga atagacgccg caccggctat tttgtgcgat cgcggacatg
    10501 catcacgttc gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt acggactacg
    10561 cttaaaaaaa ccttcttcgc gaggcgatac catgagccga ttcatcccca tagaattaca
    10621 ccatgccagc cggctgttaa atcatggccc aactgtcatg atcaccagtt tcgatgagca
    10681 atcccagcgg cgtaacatta tggctgcagc ctgatcaatg ccggtggagt ttgaacctcc
    10741 acgcgtgacg attgtggtag ataaatcgac atggaccagg gagttgattg aacgtaacgg
    10801 taaatttggc atcgttatcc cgggcgttgc agcaactaac tggacgtggg cagtgggaag
    10861 tgtgtcgggt cgtgaagaag ataaatttaa ttgctatggc attccggttg tgagaggccc
    10921 ggtatttggt ttgcctctgg tcgaggaaaa atgtctggcg tggatggagt gtcgattgct
    10981 acctgcgact tctgcgcaag aagaatacga cacgctgttt ggcgaagtag tatcagcagc
    11041 ggcagacgca cgggtatttg tcgaaggccg ctggcagttt gatgatgata agctcaatac
    11101 gttgcatcat ttaggtgctg ggacgtttgt taccagcggc aagcgtgtta cggcgggtta
    11161 agcattacat gctgtaaccg ggtttcttta tcaaagcctc catttggggc gcgatttcgg
    11221 catcccagat agcttgccaa gattctggtt gaatctgttg tagagcaatg cttatcgaac
    11281 tgtctttact gttcagatgg cgaataataa cgtcggtaat atctgcagca agtgctgctt
    11341 tttgttgttc gtccagttca cgcggaaaac atttaatgtc tatgtgcggc atgattacgc
    11401 tccttttgaa agatgtcagt aaaattaaca cacattgtga atgtcgtgtg tccggtaaag
    11461 cgtttttacg acaataa
//
LOCUS       DAZGXS010000133        11417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGXS010000133 DAZGXS010000000
VERSION     DAZGXS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            242..541
                     /gene="yoaC"
                     /locus_tag="ACHKG5_003869"
     CDS             242..541
                     /gene="yoaC"
                     /locus_tag="ACHKG5_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0869874.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHKG5_003870"
     CDS             complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHKG5_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0869875.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            914..2275
                     /gene="pabB"
                     /locus_tag="ACHKG5_003871"
     CDS             914..2275
                     /gene="pabB"
                     /locus_tag="ACHKG5_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0869876.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2279..2857
                     /gene="nudL"
                     /locus_tag="ACHKG5_003872"
     CDS             2279..2857
                     /gene="nudL"
                     /locus_tag="ACHKG5_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0869877.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHKG5_003873"
     CDS             3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHKG5_003873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0869878.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHKG5_003874"
     CDS             4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHKG5_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0869879.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHKG5_003875"
     CDS             complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHKG5_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0869880.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHKG5_003876"
     CDS             complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHKG5_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0869881.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8157..9128
                     /gene="manX"
                     /locus_tag="ACHKG5_003877"
     CDS             8157..9128
                     /gene="manX"
                     /locus_tag="ACHKG5_003877"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0869882.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9191..9991
                     /gene="manY"
                     /locus_tag="ACHKG5_003878"
     CDS             9191..9991
                     /gene="manY"
                     /locus_tag="ACHKG5_003878"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0869883.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10004..10855
                     /gene="manZ"
                     /locus_tag="ACHKG5_003879"
     CDS             10004..10855
                     /gene="manZ"
                     /locus_tag="ACHKG5_003879"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0869884.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10910..11368
                     /gene="yobD"
                     /locus_tag="ACHKG5_003880"
     CDS             10910..11368
                     /gene="yobD"
                     /locus_tag="ACHKG5_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0869885.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2805 a         2875 c         2865 g         2872 t
ORIGIN      
        1 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
       61 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatatcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      541 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      601 ctttttctca caatcaaata atcatcagct tctataacga aatcgatacg gattagtgtg
      661 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      721 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      781 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      841 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      901 agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga
      961 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1021 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1081 tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1141 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1201 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1261 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1321 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1381 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga
     1441 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1501 atttcgccgg gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1561 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1621 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1681 gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga ttaaaggcac
     1741 gctaccacgc ctgcccgctc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1801 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1861 tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc
     1921 cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga
     1981 tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat
     2041 ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca gcattggcta
     2101 tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga ctgccattaa
     2161 cggacaaata tactgctctg cgggaggtgg aattgtcgcc gatagccagg aagaagcgga
     2221 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2281 ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca
     2341 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg
     2401 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2461 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2521 gcgcgaagct gaagaagagg ttgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2581 gccgcccgtc gatagcgtaa ctggctacca ggtaacccca gtggtcggca ttatcccgcc
     2641 cgatctgccg tatcgcgcca gtgaagatga agtttccgct gtgtttgaaa tgccgctcgc
     2701 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgcg gcgattcaca
     2761 tcgggtatgg ctatcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2821 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2881 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     2941 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3001 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3061 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa
     3121 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt
     3181 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3241 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3301 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt
     3361 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3421 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3481 cggcggtttt atcgtcgatg aagaacactt tggtcaggat actgccaacg aagtgagcgt
     3541 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3601 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3661 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3721 aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgct
     3781 ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3841 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3901 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     3961 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4021 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4081 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4141 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4201 ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc
     4261 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac
     4321 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4381 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4441 atttatcccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4501 ggcgtttgtc gccatacgct tacagagtgg cccgcatgca aaaagcacaa cggatcatta
     4561 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4621 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4681 tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg
     4741 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4801 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt
     4861 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4921 caccgcaacc acttttacgc ctgacgaccg accgtgtcct gattaaaggc agtccggttt
     4981 tgattcaatg gacgcctgca gcgagcagta gcaatgctgg ggtcatggag atgattaaca
     5041 tcgacttact gacggcaatg ctgcttgagc cacaactgcc gcaaatcagt agcgccagcc
     5101 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccacaac
     5161 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt ttcttttacc attaacgtta
     5221 atggtccggg ggctccggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5281 tactgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cgaatgagct
     5341 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5401 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc
     5461 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5521 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5581 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5641 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5701 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc
     5761 gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc
     5821 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5881 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     5941 ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg
     6001 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6061 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6121 tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6181 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6241 atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6301 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6361 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6421 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac caagccttgt
     6481 accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc
     6541 agcaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc
     6601 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg
     6661 atgatttcat ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc
     6721 tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6781 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6841 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6901 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     6961 cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact
     7021 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7081 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7141 accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7201 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta
     7261 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gaccacccag
     7321 aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc
     7381 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7441 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7501 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag caaacgcgct
     7561 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7621 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7681 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7741 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt
     7801 taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat
     7861 actccatgat tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7921 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     7981 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8041 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8101 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8161 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8221 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8281 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8341 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8401 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8461 taatggctcg tgatgatgac ccaagctttg atgaactggt tgcgctggca gtagaaacag
     8521 gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg ccagcacccg
     8581 ctgccgcagc acccaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8641 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8701 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8761 ctgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8821 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc
     8881 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     8941 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaagtgaat aacgcggttt
     9001 cggttgatga aaaagatatc gaggcgttca aaaaactgaa tgcacgcggt attgagctgg
     9061 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9121 ataagtaacg tattgtgttg attatcagtc agttttcaca ctttagtcat acgtaaacag
     9181 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9241 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9301 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9361 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9421 atctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtattgc
     9481 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9541 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9601 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9661 cgttgcgctg tctgtaggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9721 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9781 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9841 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9901 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg
     9961 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10021 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggagtcttcc tgcgttctaa
    10081 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10141 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10201 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10261 cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10321 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10381 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10441 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10501 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10561 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10621 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10681 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10741 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10801 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10861 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10921 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    10981 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11041 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11101 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11161 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11221 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11281 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaagaat
    11341 ttataaactt ctcgtttcaa ctcaatgagt tattaattta gccaaagcta tgtttagtgt
    11401 atttttaata atcagac
//
LOCUS       DAZGXS010000134        10970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGXS010000134 DAZGXS010000000
VERSION     DAZGXS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(185..2452)
                     /gene="adiA"
                     /locus_tag="ACHKG5_003881"
     CDS             complement(185..2452)
                     /gene="adiA"
                     /locus_tag="ACHKG5_003881"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0869886.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2651..3559)
                     /gene="melR"
                     /locus_tag="ACHKG5_003882"
     CDS             complement(2651..3559)
                     /gene="melR"
                     /locus_tag="ACHKG5_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0869887.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3842..5197
                     /gene="melA"
                     /locus_tag="ACHKG5_003883"
     CDS             3842..5197
                     /gene="melA"
                     /locus_tag="ACHKG5_003883"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0869888.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5300..6721
                     /gene="melB"
                     /locus_tag="ACHKG5_003884"
     CDS             5300..6721
                     /gene="melB"
                     /locus_tag="ACHKG5_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP0869889.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6860..7489)
                     /gene="yjdF"
                     /locus_tag="ACHKG5_003885"
     CDS             complement(6860..7489)
                     /gene="yjdF"
                     /locus_tag="ACHKG5_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0869890.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7612..9258)
                     /gene="fumB"
                     /locus_tag="ACHKG5_003886"
     CDS             complement(7612..9258)
                     /gene="fumB"
                     /locus_tag="ACHKG5_003886"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0869891.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(9336..10676)
                     /gene="dcuB"
                     /locus_tag="ACHKG5_003887"
     CDS             complement(9336..10676)
                     /gene="dcuB"
                     /locus_tag="ACHKG5_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0869892.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2568 a         2682 c         2908 g         2812 t
ORIGIN      
        1 tttctcatgt acatattttt ttacagggta aataacaggc aaaaaaaagc gcggggtggg
       61 gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact
      121 caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata
      181 gtggttacgc tttcacacac ataacgtggt aaataccgtc aataatttca gtcccttcag
      241 tttcgtgttc aaatccaggg aaatgatggt cccaggattg cagcgagcgt aaataactta
      301 cttgcggact gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg
      361 gatacgggat aactgagttt gccgcgatac gtcctggcag attttcaatg gataccagtt
      421 cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca
      481 ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca
      541 tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa
      601 gttccggcat cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa
      661 gggtgttaac cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa
      721 tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg accaagccag gcagtgacca
      781 gcgccgccgg aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga
      841 tgctgacttt aatcgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc
      901 agctttcgcc cggatgcatt acccagcagt cctgaacggt ggtcagcagt ttggttggtg
      961 cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg
     1021 gtttgaagaa ccagctaccg tcagcggcga actctttata cagccgcgcc atcgcctgac
     1081 ggaaatcaac cgcttcgtcg atcacttcct gcgtcagtga caggccgctg ttgccgtcca
     1141 tcatcgacac cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat
     1201 gcatcatgta ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat
     1261 gaatataaga ggcctgtgaa agcgcattca gcagtttgtg ggtggagtgg gtggcgaaaa
     1321 cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg
     1381 ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt
     1441 ccagcagatc ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtca
     1501 ccacgcagta agacggtttt tgcccggctt tgtctttagt cagcgggctt tcactgattt
     1561 tcttctgcaa ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc
     1621 ggttgcggct tggcaccatg tagaccggtt tcgcgcctgt cagcatcaaa ccttgttcga
     1681 tggatttatg gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca
     1741 tgatggtgcg gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa
     1801 atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac
     1861 cgagggaagt tcgttcgatg cccatgtcgg tgcggaacag attttcaccg tagtagtcat
     1921 ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg
     1981 cccaggaata ttcatggatg tcgctatatt tcatcagcgc gctgaacagt ggcggcaaca
     2041 gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggcggtat
     2101 cttccagaat ccaggcgaat tcatcgacaa gctccagcag atcccggtcc attgcggcga
     2161 gggctttttc ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct
     2221 taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca
     2281 tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg
     2341 atttaatcac ggtaacattt tgctggctta aagcatctgc cagacgctca acagcgttac
     2401 cgacccaggt gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt
     2461 acccggttat gaaggaatct tcgttgcggg caagtatctt ccggcctttt cgggctgtaa
     2521 agcgcgtgaa aaacaaacat ttttgaaata tttgttttta taaacaatat gttatgtgat
     2581 tattttaaat tattttcact gcttattatc gacggctaaa ctattttttt ggctgatact
     2641 gatatcgtct ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt
     2701 gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc
     2761 ctgccgtcag ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt
     2821 ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata
     2881 tccccattgc atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg
     2941 cctggtcata gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc
     3001 gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagccag
     3061 agaggctaaa tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct
     3121 cattcggact gtttaattcc tgctgccagc ggcgcacttc aaacgggcta agttgctgtg
     3181 tagcaagtga tttgatcacc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg
     3241 gccaggagag aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg
     3301 tatctgttag ttggtgcggt atacaggccc agaacagcgt gatatgtccc tgattgatat
     3361 tcactttttc attgttgatc aggtattcca catcgccatc gaaaggcaca ttcacttcga
     3421 cctgaccatg ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc
     3481 gctggtattc cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca
     3541 taaacgtatc tgtattcatg gatggctctc tttcctggaa tatcagaatt atggcaggag
     3601 tgagggagga tgactgcgag tgggagcacg gttttcaccc tcttcccaga ggggcgaggg
     3661 gagtgtccga gcatcatgag gccgaaaact ctgcttttca ggtaaattat tcccataaac
     3721 tcagatttac tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc
     3781 ggaggttttc tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg
     3841 catgatgtct gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa
     3901 tattcttggt gatgtgttcc atcgcgaggc gctgaaaacg gcgcatattg ccctgatgga
     3961 cattgacccc acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc
     4021 aggggccagc ggcaaaatca cctgccacac ccaacagaaa gaagccttac aggatgccga
     4081 ttttgtcgtg gtggcatttc agattggcgg ttatgcacct tgcacggtga ctgatttcga
     4141 ggtctgtaag cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat
     4201 tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt tgcgaggaca tgacggaagt
     4261 ctgccccgat gccaccatgc tcaactatgt taacccaatg gcgatgaata cctgggcgat
     4321 gtatgcccgc tatccgcata tcaaacaggt cgggctgtgc cattcggtgc agggaacggc
     4381 ggaagagctg gcgcgtgacc tcaatatcga cccggctacg ctgcgttacc gttgcgcagg
     4441 tatcaaccat atggcgtttt acctggagct ggagcgcaaa accgccgacg gcagttacgt
     4501 gaatctctac ccggaactgc tggcggctta tgacgcaggg caggcaccga agccgaatat
     4561 tcacggcaat actcgctgcc agaatattgt gcgctatgaa atgttcaaaa agctgggcta
     4621 tttcgtcacg gaatcgtcag aacattttgc tgagtacaca ccgtggttta ttaagccagg
     4681 tcgtgaggat ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt
     4741 cgagcagctg gcgaactggc ataaagagct ggaggagtat aaaaacgcct cccggattga
     4801 tattaaaccg tcacgggaat atgccagcac aatcatgaac gctatctgga cgggcgagcc
     4861 gagtgtgatt tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg
     4921 ttgcgtggaa gtggcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac
     4981 gctaccttcg catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac
     5041 cgaagctatt cttacggaaa atcgcgaccg tgtttaccac gctgcgatga tggacccgca
     5101 tactgccgcc gtgctgggca ttgacgaaat atatgctctt gttgacgacc tgattgccgc
     5161 ccacggcgac tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc
     5221 cgggggattt attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt
     5281 aacagcgacc cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg
     5341 agcgttcggg aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac
     5401 cgatgtcgtc gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg
     5461 ggatgctatt aacgatccga ttatgggatg gattgtaaat gctacgcgat cgcgatgggg
     5521 taagttcaaa ccctggatcc tgatcggtac gttggcaaac tctgtaatct tatttctcct
     5581 ctttagtgcg catctgtttg aaggtactac tcagattgcc tttgtttgcg tgacctacat
     5641 cctctggggc atgacttaca ccattatgga tattcccttc tggtcgctgg ttccaaccat
     5701 cacgctcgat aaacgtgagc gtgaacaact ggttccttat ccgcgttttt ttgccagtct
     5761 ggcgggcttt gttacggcag gtgtgacgct accatttgtt aattatgtcg gcggtggcga
     5821 tcggggattt ggctttcaga tgttcacact ggtactgatc gcctttttta ttgtttcaac
     5881 catcatcact ctgcgcaatg tgcatgaagt cttttcgtca gacaatcaac cgtctgctga
     5941 aggaagccat ctgacactta aagccatcgt tgcgcttatt tataaaaacg atcagctttc
     6001 atgcctcttg ggtatggctc ttgcttataa tgtagccagc aacattatta ccggctttgc
     6061 tatctattat ttctcatatg ttatcggtga tgcggatttg ttcccctatt atctgtcgta
     6121 tgcgggagct gctaacctgg tgacgttagt attcttccca cgcttagtta aatcattatc
     6181 ccgacgcatt ttatgggccg gagcatctat tcttccggtg ttaagctgtg gtgttctcct
     6241 gttaatggca ttaatgagct atcacaacgt cgtcctcatt gtgattgcgg gtattttgct
     6301 gaatgtggga acggcgcttt tctgggtatt acaggtcatc atggtggcag ataccgttga
     6361 ttacggtgaa tataaactgc acgtacgctg tgaaagcatc gcttactccg tgcagactat
     6421 ggtggtgaag ggcggttcag cctttgcggc ttttttcatt gcggttgtgt tagggatgat
     6481 tggctatgta ccgaatgttg aacagtctac gcaagcccta ttaggtatgc agtttattat
     6541 gattgctcta ccgactctgt ttttcatggt aacgctgatt ctctacttcc gtttctatcg
     6601 cctcaatggc gacacgctgc gcaggatcca gattcatctg ctggataaat atcgcaaaat
     6661 accgcccgag cctgttcatg ctgatattcc ggtcggtgca gtgagtgatg tgaaagcctg
     6721 acgtggacaa tagctaacga aaatggcctg atgcgatatg tttatcaggc cacgtcttaa
     6781 tggaatgctt tggatttgca aattttgtag accggataag gaattcacgc cgcatccggc
     6841 atcaacaaag tgcacgttgt tatccggtta tcaagccaaa gcgccgtagc tgacggcaat
     6901 gaaagcgagc gaggaatatc accgtggtta atgcgccaag cagcgcgcag aacatatcgg
     6961 attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc atccgctccc tggcccattg
     7021 ccagcgccgc ccaccactcg attaattcat acatcgcgct tatcgccagc gccacgcaac
     7081 agaccagaaa cgccaccatt ttacgtccgc gaacgtacat cccgcgcaca aggatctctc
     7141 gtgccaccag tgcaggcacc agcccctgga aaaaatgccc cagcttgtca tacggattac
     7201 ggctcaaccc cagccattcc tgcacctcaa aaccgatggg gactttcgcg taggtgtatt
     7261 gcccgccgac catcaggatg atggcgtgaa agaaaatgag cgtatagagc agcggcgtta
     7321 acggataacg tttagcggtg gcaagcagta gcggcacgac aataatcacc ggtgtcactt
     7381 ccatcagcca ggtgagtttg tcatgggccg aaatgccggt ataaatcagg attaacgtta
     7441 gcgccagtgc gccggtgtta agaattaacg gcttgagtgt gcgggtcatg gtgagttcgc
     7501 aagtcaggga aaatactgac tattcaccag caagcgtgaa attacaatcg caaaagaggt
     7561 ggcccagggg gatcacctgg cagcatgctg ccaggcgctg ggccgaagag gttacttagt
     7621 gcagttcgcg cactgtttgt tgatgatttg ctggaagaag tcgttacctt tgtcatcgac
     7681 caggataaac gccgggaaat cttctacttc gattttccag atagcttcca tacccagctc
     7741 cggataagcg acgcattcca gatgcttgat gctctgctgc gccagtaccg ccgccggacc
     7801 gccgatgcta ccgaggtaga agccgccgtg tttatgacac gcgtcggtaa cctgctgact
     7861 gcggttacct ttcgctagca tgatcatgct gccgccgtgg gattgcagca gatccacata
     7921 ggagtccata cgacctgcgg tggttgggcc aagtgagcct gatggataac cggcaggggt
     7981 tttcgccgga cccgcgtagt agatcgggtg atctttgatg tactgcggaa gttctttacc
     8041 ggcgtcaatc agctctttca gcttggcgtg tgcaatatct cggcccacga taatggtgcc
     8101 ggtgagcgac aaacgagtgg aaaccggata ttgcgaaagc tgggcgagga tctctttcat
     8161 cgggcggtta aggtcgacct tcaccgcttc gccttcaccg gcctggcgca gttcttgtgg
     8221 aatgtactga cctggatggt gttccagttt ttcgatccag ataccttcgc ggttgatttt
     8281 cgctttaatg ttacggtcag cggagcagga gacgcccatg cctaccgggc aggatgcgcc
     8341 gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag tatttaccgc caaactgcgc
     8401 gccaagaccg agtttctggg cctcttccag cagttcctgt tccagctgga catcgcggaa
     8461 cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg taatagtgag cgcttgctaa
     8521 cttaacggtt ttcaggttgg tttccacaga cgtaccgcca atcacaaacg cgatatggta
     8581 cggcgggcag gctgcagtac cgagggtacg cattttctcg acgaggaagt ttttcagttt
     8641 gccgggagtc agcagggctt tggtttcctg gtagagatag gttttgttgg cagagccgcc
     8701 gcctttcgca acgcaaagga atttgtactc atcgccatct accgcgtaca ggtcgatttg
     8761 cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg tccagcgccg cgttttgcga
     8821 gtagcgcagg ttatcttcga tataggtgtt atagacgcct ttcgacagcg cttcttcatc
     8881 accgccaccg gtccacacgc gctggccttt tttaccgacg atgatcgcgg tgccggtatc
     8941 ctggcaggtc ggcagcacgc ctttggcggc gatttcggag tttcttaaga attgcagtgc
     9001 cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa cctgtttctg
     9061 gtgtgccgga cggagcatga aagaagcgtc gtggaaggct tgctgcgcca gcagggtcag
     9121 ggcttctggt tccactttca ggatggtttc gccgtcgaag tcggcaacgc taacgtaatc
     9181 ggaagtgagt agatagtatt cggtattgtc tttccccatc gggaaaggtg cctggtagat
     9241 aaagggtttg tttgacatag cttccagcct gtaactctgt atttgttatt cgaaaatggc
     9301 gtgccgcgta gtaacgggcg gcacgcaaag tgcatttata agaacccgta catcgcggcg
     9361 aagatccagc cgaagacgca cgatacgctc acaccaatca accccggcag aataaagctg
     9421 tggttgatga cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg aatcgctgcc
     9481 agatcgctcg gataagtcgg caggatgtaa taaccgtagc aagccggtgc tgaagccacg
     9541 atgtatgccg gatcaacgcc gatcgctagc gcgaccggaa caatcgccgc cagcgccgca
     9601 gcctgagagt ttacaaactt ggaaaccagc agcagaacaa tggcataggc ccacggatac
     9661 tctttcacca tttcacccag tacgccctga atttcagaca tatgcgcacc gaacatggtt
     9721 tctgccatcc atgcgatacc gtacaccgcc acgatggcga tcataccgga acggaagact
     9781 tcgttttttg agatagacgc gggattggtt ttggtcagga taataatcag cgccccggtc
     9841 agcagcataa acatctgaat aaccagtacc atcgacagcg gtttgccgcc gaaggatgga
     9901 cgcaggtccg aatcagcacc aagaagtgcg actacagcga ttgccccgag gaaaatccac
     9961 attgccagcc agttgctttt cggcagtttt ttatccagca gcgtcgcggt atcaccgtaa
    10021 acatactcac ggttttccgg tacggagatg aatttctgga actcttcgtc tttatccaga
    10081 tctttaccgc ggaaccagct gaagataccg atcgccagga taccgattaa cgtcgatgga
    10141 atggtgattg ccagcaggtc gaggaactca agatggcgac catcaaaggt gacattaccc
    10201 agcattgcaa ccagagacac gaccgcaacc gacaccggac tggcgataat ccccatctgt
    10261 gcaccgatag aacttgccgc catcggacgt tccggacgga tgttgttctt aatggcgacg
    10321 tcgtagatga tcggcagaat ggtgtaaacc acatgacccg taccgcaaag aatggtcagc
    10381 gtacaggtca caaacggcgc gacaattgag acatatttcg ggttgcggcg cagcagcttc
    10441 tcggcaattt gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc cgatgccgcc
    10501 accaccgcaa tgataaccag catgacatca actggtggtt tacctggctg aaggtggaag
    10561 acgaagacca gaatgaccag accgataccg cctaataaac ccagcgcgat accacccttt
    10621 ctggcaccat aaaacagaca tatcagtatt atgataagtt ggatagtaaa taacatgtgt
    10681 gaaccctcgc gataatccta tttaaatttt tgctgaatag atcacagtca cgttctgttt
    10741 tgtatgaact gttttcagag cctgactaaa tatccgtttg gtcgttaccg gctttagcaa
    10801 atacctcaca gtgaatattg gctgattaaa gcagcggcaa atttatgcac tgtgataagc
    10861 ggctttttca gagagaaaaa ctctctggcg atgggtttaa agtttgtact gcgcagatgt
    10921 taatgttttg tttgctgtta tcttattgat atttatcggt ctattttttg
//
LOCUS       DAZGXS010000135        10897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGXS010000135 DAZGXS010000000
VERSION     DAZGXS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..2308)
                     /gene="rhsC"
                     /locus_tag="ACHKG5_003888"
     CDS             complement(<1..2308)
                     /gene="rhsC"
                     /locus_tag="ACHKG5_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFP0869893.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQH"
     gene            complement(2468..2680)
                     /gene="rpmE"
                     /locus_tag="ACHKG5_003889"
     CDS             complement(2468..2680)
                     /gene="rpmE"
                     /locus_tag="ACHKG5_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0869894.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            2883..5081
                     /gene="priA"
                     /locus_tag="ACHKG5_003890"
     CDS             2883..5081
                     /gene="priA"
                     /locus_tag="ACHKG5_003890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0869895.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            5237..6262
                     /gene="cytR"
                     /locus_tag="ACHKG5_003891"
     CDS             5237..6262
                     /gene="cytR"
                     /locus_tag="ACHKG5_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0869896.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6354..7313
                     /gene="ftsN"
                     /locus_tag="ACHKG5_003892"
     CDS             6354..7313
                     /gene="ftsN"
                     /locus_tag="ACHKG5_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0869897.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7406..7936
                     /gene="hslV"
                     /locus_tag="ACHKG5_003893"
     CDS             7406..7936
                     /gene="hslV"
                     /locus_tag="ACHKG5_003893"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0869898.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            7946..9277
                     /gene="hslU"
                     /locus_tag="ACHKG5_003894"
     CDS             7946..9277
                     /gene="hslU"
                     /locus_tag="ACHKG5_003894"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0869899.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQLQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            9344..10270
                     /gene="menA"
                     /locus_tag="ACHKG5_003895"
     CDS             9344..10270
                     /gene="menA"
                     /locus_tag="ACHKG5_003895"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0869900.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            10363..10848
                     /gene="rraA"
                     /locus_tag="ACHKG5_003896"
     CDS             10363..10848
                     /gene="rraA"
                     /locus_tag="ACHKG5_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0869901.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2462 a         2968 c         3036 g         2431 t
ORIGIN      
        1 catgctgcca gagcagtgct tccgtctgcg ggtgatgcac cgtctgacgc tcaccggtca
       61 gccggccttt ctcatcatac ccgtaatgca ccgtcacccg gtgcccttcg ctgatatggc
      121 tgatgtctgt cagccagccg cgttcgtcat actgccagcg ctctgcggtt tcacccttca
      181 cggtgcggtg cgtgaggcgg tctgcttcgt catagtgcca gtgggtgacc agcccctcat
      241 cctcgctgcg gataagtttg ccggtcaggt cgtggtgata acgctgtgtg cggccgtcaa
      301 agccggtttc ctgtatcagc cggtcgagta catcgtaacg gaaggtggtg tggctgccgt
      361 tttcactggt caggcggatg acccgtccgg cagcatcgta ttccatactg cgcgtcagac
      421 cgccctgcgt ggtacagatg gcttttcccc acgcatcgta ctgtgtcccg tttctgctgc
      481 cgtccggggc aatgacggtg gtcaggtcac cggcggcgtt gtattcatac cgcgtttcat
      541 ggccctgcgt gtctttcacg gcaattaact gtccacggct gtcgtatgcg cggtactgac
      601 tcagcccttc ctcgcggtgc accgccgtca tctgcccgaa gcggtcatgg tcataacggg
      661 ttacataacc ggaacagtcg gtgaagctca gcaactgacc gtaacggctc cacgtcatgg
      721 ttttccggct gccggtggca tcttccgttg cgcagggtaa gtcactgtgt ggattatcat
      781 aacggtagcg ggtgatatcg ccgtcagggg cagtttcctg aatcagacgg ccccattcat
      841 catattcccg gcgtatttcc agcccgtcag gcccggtggc tgacgttaac tggctgtggt
      901 ggttatagta aaacgccgat gccctgccat ccggcgtggt gatgcgcgtg atgaggcccg
      961 tcaccacatc cgggctgtat tctgttgtcc tgcctgcggc atccgtctgt gccctgagcc
     1021 tgcccaccgc gtcaaactga ctctgcgtga cgctgccgtc cgcgtgttcc tttttcacca
     1081 cccgcttcag cccgccttca ccctgcgtgt gcaggacttc acggcggttc aggctgtcgg
     1141 tgatggtgat gcggtctttc tcatactgat acgtgtagct taagcctgcc gggtttagct
     1201 gttctgtcac ccgcccgtcg ctgtcgtaac ggtaacggat ttccggccgg cccgtgtgac
     1261 ggtgcgccac catccggccc cggtatttat catcgtaagt aaagctgcgc acctgtttgc
     1321 cactacggtc atacaccacc gccagttcgc cgcgcggcgt ccagccatag cgcaccagcg
     1381 gcgcggcagg taaattctcc gggtattccg ggtcgtgcgt cagccacacg gcagacagac
     1441 ggatgccgtt gtcccggcca tattcggtgt aacccggcag ggtatcagga aaagcggacg
     1501 gttccgtccc gccggaaatg gcctgctgcc gggcttcttc tgcccgctgc gcctgcgtgg
     1561 tcagtaccag ccggaagtga cgcccggcac catccgtcac gccggtgatt tcgccgctga
     1621 attcaccggc ggcttcgcgg tggaacgtct gtgtgcgccc gaagcggtcc accagcccgg
     1681 tcagtacccg gtacggcggc agcggcgcag gcagcacctc atccgcttcc ggcacccgct
     1741 cacaccagcc gagcagccac cacggcccct gcggactgtt tgtcgccaga taacgatgcg
     1801 gacttaagcg gagttcttcc ggcagcgcct gccagagtgc ggccagccgg tgaccttcat
     1861 ccagtttcgc cacgccgccg cgcaccagcc agagtgactc gctgcggctg taaccgtcct
     1921 caccgggaaa caggtgctca aaatacaggc ttctgccgcc gttatcactg agtatcagtg
     1981 tgttatcgcg cagctgtaag cggatatccg caggcatttt ccagccgggg ccgaggctcc
     2041 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
     2101 gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat
     2161 tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg ccggtggggg
     2221 caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc
     2281 cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag
     2341 aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag
     2401 aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa
     2461 tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac
     2521 cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga
     2581 ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag
     2641 cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg
     2701 ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa
     2761 tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg
     2821 atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg
     2881 ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctcc
     2941 tgccagaagg catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc
     3001 aggagcgcat cggggttgtg gtgtcagtta gcgacgtcag tgagctgccg cttaacgagc
     3061 taaaagcggt agtcgaagtg ctggatagtg agccggtgtt tactcactcc gtctggcgat
     3121 tgctgctctg ggcggcggat tactatcatc atccgattgg cgatgtgctg tttcatgcct
     3181 tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg
     3241 ccactgaaca aggccatgcg gtggatctga acagcctgaa acgttcgcca aagcaacaac
     3301 aggcactggc ggcattgcgg caaggcaaaa tctggcgcga ccaggttgcc acgcttgaat
     3361 ttaatgatgc cgcgttacag gcgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa
     3421 caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgaacgg ttgcgattga
     3481 ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcggatacc ttctctgctt
     3541 ggctgctggc gggcgttaca ggttccggta aaacagaggt ttatttgagc gtactggaaa
     3601 acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc
     3661 aaactatcgc ccgttttcgt gaacgtttta atgcccccgt agaagttctg cattccggcc
     3721 tgaacgacag cgagcgtctt tcggcgtggt tgaaagcgaa aaatggtgag gcggcgattg
     3781 tcatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggtgtg attgtcattg
     3841 atgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc
     3901 tggcggttta tcgtgcgcac agcgagcaaa tcccgattat tcttggctcc gcaacacccg
     3961 cactggaaac gttatgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgtc
     4021 gggcggggaa tgcgcgtccg gcaattcaac atgtgctgga tttaaaaggt cagaaggtgc
     4081 aggcggggct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccgacaacc
     4141 aggttatcct ctttcttaac cgccgtggct ttgcgcctgc cttgctgtgc cacgactgcg
     4201 gctggattgc cgaatgtcca cgttgcgatc actactacac gctgcatcag gcgcaacagc
     4261 atttgcgctg tcaccactgt gacagccaac gcccggtgcc gcgccagtgc ccttcctgcg
     4321 gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgctcgcgc
     4381 cgttattccc tggtgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaaggag
     4441 cgctggaaca acaactggcg gaagtgcatc gcggcggcgc gaggatattg attggcacgc
     4501 aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtagcgtta ctggatgtgg
     4561 acggcgcgct gttttctgcc gattttcgct cggcagagcg ttttgcccag ctatacaccc
     4621 aggtagcagg ccgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcatc
     4681 atccggaaca cccgctattg caaacgttgc tctataaagg ctacgacgct tttgccgaac
     4741 aggcactggc tgagcggcga atgatgcaac tgccgccgtg gaccagccat gtgattgtgc
     4801 gtgcggaaga tcataacaat cagcacgcgc cattgttcct gcaacaactg cgtaatctga
     4861 tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg
     4921 cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc
     4981 gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta
     5041 aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc
     5101 ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc
     5161 gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc
     5221 caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt
     5281 tgccctcaag gcaaaagtct ctacagcgac cgtctcccga gcattaatga atcccgataa
     5341 agtctcccaa gccacccgta atcgggttga aaaagcggcc cgtgaagtgg gttatttacc
     5401 gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc
     5461 ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa
     5521 tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt
     5581 tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc
     5641 gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt
     5701 tgcaccggaa ctggagctgc ctaccgttca tatcgacaat ctgaccgccg catttgatgc
     5761 agtaaattat ttatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga
     5821 gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat
     5881 tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc
     5941 gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt
     6001 gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct
     6061 ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac
     6121 catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat
     6181 gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg
     6241 atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc
     6301 gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac
     6361 aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac
     6421 ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg
     6481 ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg
     6541 agacgctgca aagccagaaa gtgaccggaa acggactacc accaaaacca gaagaacgct
     6601 ggcgctacat caaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt
     6661 ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg
     6721 aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg
     6781 aacagacgcc ggaacagcgt cagcaaacgc tacagcgcca acgtcaggcg cagcaattag
     6841 cggaacagca acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga
     6901 cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta
     6961 ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc
     7021 cgcagcaagc tgcgccggtt actcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     7081 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     7141 cggtacgtgc tcagctggcg ttcgaaggct ttgactcaaa aattaccacc aacaatggct
     7201 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     7261 ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     7321 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     7381 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     7441 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     7501 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     7561 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     7621 ggtcaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgt tgcgcaaact
     7681 tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga
     7741 cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc
     7801 tgcggcgcgt gcactgttag aaaatactga acttagtgct cgtgaaattg ctgaaaaggc
     7861 gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt
     7921 aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga
     7981 actggataag cacatcatcg gccaggacaa cgccaagcgt tccgtggcga ttgctctgcg
     8041 taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa
     8101 tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa
     8161 gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctacgt
     8221 cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg
     8281 cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct
     8341 cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc tgcaggaacc
     8401 gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga
     8461 aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat
     8521 ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggtc agaagcaaaa
     8581 agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa
     8641 actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat
     8701 cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt
     8761 ttctcgtgaa ggcgttcagc gagacctgct gccgctggta gaaggttgca ccgtttccac
     8821 caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat
     8881 tgcgaaaccg tctgacctga tcccggaact gcaaggtcga ctgccaatcc gcgttgaact
     8941 gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac
     9001 cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg
     9061 tattaaacgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc
     9121 tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga
     9181 tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt
     9241 ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat
     9301 tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat
     9361 tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc
     9421 tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cattttgatc cgctggtcgc
     9481 cctgctggca ctgattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta
     9541 cggcgatgcg gtaaaaggca gcgataaacc tgaccgtatc ggaccgctac gcggcatgca
     9601 aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat
     9661 ctgtctctcc gggctggcac tggttgcagt ggcgtgccat acgctggccg attttgtcgg
     9721 tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg
     9781 tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttaag
     9841 cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc
     9901 gaccgcatgc ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa
     9961 tagcgaccgc gaaaacggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg
    10021 tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt
    10081 ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccgttgc tggtgaagca
    10141 agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccgatgc tggaacgcac
    10201 tgtcaaggga gcgttactga ctaacctgct gtttgttgta gggatattcc taagccagtg
    10261 ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag accacatagc
    10321 gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc
    10381 gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt
    10441 ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg
    10501 ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt
    10561 ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa
    10621 tgggaaggtc tggtcattta cggcgcggtg cgtcaggtag atgacctgga agagttggat
    10681 atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa
    10741 agcgatgtcc gcgtcaattt tggcggtgtt accttcttct ccggcgacca tctttatgcc
    10801 gacaataccg ggattattct ttcagaagat ccgctggata ttgaataagt atctgccatg
    10861 cgttaaaagg ggaattaaat tccccttttt tatacat
//
LOCUS       DAZGXS010000136        10861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGXS010000136 DAZGXS010000000
VERSION     DAZGXS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="ACHKG5_003897"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="ACHKG5_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0869902.1"
                     /translation="MRICSDQSCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="ACHKG5_003898"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="ACHKG5_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0869903.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="ACHKG5_003899"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="ACHKG5_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0869904.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="ACHKG5_003900"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="ACHKG5_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0869905.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHKG5_003901"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="ACHKG5_003901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0869906.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHKG5_003902"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACHKG5_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0869907.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHKG5_003903"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACHKG5_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0869908.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHKG5_003904"
     CDS             complement(7779..8657)
                     /gene="rdcB"
                     /locus_tag="ACHKG5_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP0869909.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8654..>10861)
                     /gene="crfC"
                     /locus_tag="ACHKG5_003905"
     CDS             complement(8654..>10861)
                     /gene="crfC"
                     /locus_tag="ACHKG5_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP0869910.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     IEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQDDSEPLSE
                     IRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2713 a         2854 c         2680 g         2614 t
ORIGIN      
        1 tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaatc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gttaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaac
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccattct caccctgcca
      361 tgcctgacac cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact
      421 gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacaccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccaccg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gtgtgcaggc
     1441 cgttgccacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gtttcgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc
     1561 aattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caacgtccct atagccacca ttggtggggt
     1681 attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgccggg gccatcgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacattg ctggcgggtc aaacggcgaa agcggctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag aattcggtct
     2041 ggtttcttcc gtgtcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actgctgctc ggacacggtc actttggtaa agcacgcccg gcatatctgg cggttactac
     2161 cattgccttc ctctactgca tctgggccgt ggtggggtcc ggggcgaaag aggttatgtg
     2221 gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca accggctgca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca
     2341 aagataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga
     2461 atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct
     2521 tttttaaaca ggtgttgcag gcgctgccgc tggactcgct gcataacgta ctggttttct
     2581 tgtcgatgcc ggtcgtagcc ttcagcgtga ttaatattgt cctgacacta agctctttct
     2641 tatggcttaa tcgaccgctg gcctgcctgt ttattctggt tggcgcggct gcacaatatt
     2701 tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca
     2761 ctccggcaga aagctatgcg ctgatgacac cgcaaatgtt attaacgctg ggattcagcg
     2821 gcgtgcttgc tgcgctgatt gcctgctgga ttaaaatcaa acctgccacc tcgcgtctgc
     2881 gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt ttgctggtcg
     2941 ccgcactatt ttataaagac tacgcctcgt tgttccgcaa caacaaagag ctggtgaagt
     3001 ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg
     3061 caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccatta atgcagaacg
     3121 aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct
     3181 ccctcaacgg ctacccgcgt gaaactaacc cgcggttggc gaaagataac gtggtctatt
     3241 tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg
     3301 atatgccgcg agagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg
     3421 cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg tcggaggatt
     3901 atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg caggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaatata ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgacgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta catgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa catgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gcttgtcgcg aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccatcgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttat ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggccgt tgaagatgaa ggaccgggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtt acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgttg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaacca ccgccactac
     6241 catcaggtaa taggctggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc
     6841 cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagcgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acaccaaatt tacagagaag
     7801 atttcctcga gcattgcccc cgccagacgc tgcgctgaac agagtcgtgg catccccagc
     7861 gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg cgggcttccc
     8101 agacgacagg catcgccaaa tgccccccaa cgcaactctt cgagaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgttcacccg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagtgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcagacg cagactaagc
     8461 ggcgcattta acatcccgcc gttcgtccat gtctgaagct gcacgcgcgc aagaagttcc
     8521 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcggggataa accgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagccgtgag tcatcacagt ctgctgcaat tgctgttgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat ttctcatttc
     9001 cccgtcccca gtcaggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaatc
     9061 gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg gttaacaccc
     9241 ggcaaagggt ggattcaagc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct
     9301 gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc tccgaatcat
     9361 cctgaaacaa ggcggctaac tgcaccttca gcgcatcttg ctgttgacgc agaaagtggt
     9421 tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat
     9481 tgagttgcaa tagctgcaaa ctttcttcga tctggtggat attttgccgc aattgttcac
     9541 aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat
     9601 tcaacagttt atgcgcggca gatcgcagag catacaacga ggcgttagcg taagcggcat
     9661 gaagcaacat ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat
     9721 gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat
     9781 gggcaaaatc ttccacccag cgttgttgct ctggcggtgg taacttaccg ttgttggcta
     9841 actcatggcg cgcccgattt gccaggtagc cccacatcga cgacaccgga aatatctgct
     9901 gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt
     9961 cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt
    10021 gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat
    10081 aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca
    10141 gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaat tgaccgggat
    10201 aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg
    10261 cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc accaaatcat
    10321 tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt tcgaaagcga
    10381 cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg
    10441 tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa tttttgaatt aaacaatcga
    10501 ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg gccgggcgta tggcgaataa
    10561 gcgtcggcag cgcagtcatt gggcgattgc gattaggcag tacctccgta ccaacaatgg
    10621 cattaatggt ggttgatttc cctgctttca tggtaccgac aatcgccagc accatttcca
    10681 gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgctcggcg atattaggct
    10741 gactccaggg taaagccagt tgtggcgcat cgtctccggg tacagagaga ggcatttttt
    10801 ccagtaactg caactgttgg cgagaaagct gtaacagccg ttcagcctcc tgacttaact
    10861 c
//
LOCUS       DAZGXS010000137        10850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGXS010000137 DAZGXS010000000
VERSION     DAZGXS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            39..653
                     /gene="evgA"
                     /locus_tag="ACHKG5_003906"
     CDS             39..653
                     /gene="evgA"
                     /locus_tag="ACHKG5_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0869911.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            658..4251
                     /gene="evgS"
                     /locus_tag="ACHKG5_003907"
     CDS             658..4251
                     /gene="evgS"
                     /locus_tag="ACHKG5_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0869912.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4307..5452)
                     /gene="yfdE"
                     /locus_tag="ACHKG5_003908"
     CDS             complement(4307..5452)
                     /gene="yfdE"
                     /locus_tag="ACHKG5_003908"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0869913.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGLSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQTILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5526..6470)
                     /gene="yfdV"
                     /locus_tag="ACHKG5_003909"
     CDS             complement(5526..6470)
                     /gene="yfdV"
                     /locus_tag="ACHKG5_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0869914.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGIACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6540..8234)
                     /gene="oxc"
                     /locus_tag="ACHKG5_003910"
     CDS             complement(6540..8234)
                     /gene="oxc"
                     /locus_tag="ACHKG5_003910"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0869915.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8288..9538)
                     /gene="frc"
                     /locus_tag="ACHKG5_003911"
     CDS             complement(8288..9538)
                     /gene="frc"
                     /locus_tag="ACHKG5_003911"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0869916.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10051..10686)
                     /locus_tag="ACHKG5_003912"
     CDS             complement(10051..10686)
                     /locus_tag="ACHKG5_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0869917.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3167 a         2457 c         2244 g         2982 t
ORIGIN      
        1 gggagaaggg agatgcttca ttgcaaaggg aataatctat gaacgcaata attattgatg
       61 accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat attgaaatct
      121 tagcagagtt gactgaaggc ggaagtgccg ttcagcgggt ggaaacactt aagcctgata
      181 tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta gaaacgctga
      241 ggaagcgcca atatagcgga attattatta tcgtctccgc taaaaatgac catttttacg
      301 ggaaacattg tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca
      361 atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc ttctctctca
      421 accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta tcgaaacaag
      481 aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt gctgaaaaaa
      541 tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa aaattagaat
      601 gtaaatcact gatggatctt tacacattcg cacaacgtaa caaaatcggc taaccacatg
      661 aagtttttac cctatatttt tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca
      721 gacgaagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac acttgatcca
      781 ctacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct tgtgattgca
      841 gtacacaagt cccaaacggc tacgttgttg cataccgatt cgcagcaacg ggttcgtggt
      901 attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt aacactccgg
      961 gaatacgcag atcatcaaaa agcaatggac gcgctggaag aaggtgaagt cgatatagtg
     1021 ttatcacatt tagttgcttc gccgcctctt aatgatgaca ttgctgcaac caaaccactg
     1081 ataattacct ttccggcgct ggtcaccacc cttcacgatt caatgcgacc gcttacctca
     1141 tcaaaaccag taaatattgc tcgagtagca aattatcccc ctgacgaggt aattcatcaa
     1201 tcatttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt agcatccgtc
     1261 tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag tatgatttcc
     1321 cgctatttca ctcactcctt aaatgtagtg aaatattata actcaccgcg tcaatataac
     1381 ttcttcttaa ccagaaaaga atctgtcatt cttaatgaag tactcaatcg atttgttgat
     1441 gctttaacaa atgaagttcg ctatgaagta tcacaaaatt ggcttgatac aggaaacctg
     1501 gcctttctga acaagccatt agaactcact gaacatgaaa aacagtggat taagcagcat
     1561 cccgatttaa aggtgctgga aaatccttac tcgcccccct attctatgac ggatgaaaat
     1621 ggctcggttc ggggcgttat gggggacatt cttaatatta ttaccttgca aacaggttta
     1681 aatttttctc cgatcaccgt ttcacacaat atccatgctg gaacacagct gaatcccggc
     1741 ggatgggata tattacccgg tgctatttat agtgaagata gagaaaataa tgttttattt
     1801 gctgaagcct tcataacaac gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa
     1861 caaacattaa aaaaaggaat gaaagttgcc attccatatt attatgagct tcattcgcaa
     1921 ttaaaagaga tgtatccgga ggtagagtgg ataaaagtcg ataacgccag cgctgcattt
     1981 cacaaggtca aggaaggcga acttgatgct ctggtcgcga cacagttaaa ttcacgttac
     2041 atgatcgacc attactatcc taatgaactg tatcattttc ttattcccgg cgttccgaat
     2101 gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat tattaataaa
     2161 gcactgaatg caatcccccc aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg
     2221 cccaatgtga ccattgacac atgggacctc tatagcgagc aattttatat tgttacgaca
     2281 ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt acgctcagtt
     2341 cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa tatcattccg gaaagcgctc
     2401 tcggactcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt cattagtcac
     2461 aatagtgcat ttgaacatta tttcactgct gattactaca aaaatgcaat gttgccatta
     2521 gaaaacagtg aatcaccctt taaagatgtt ttttctaata cgcatgaagt cacagcagag
     2581 acgaaagaaa accgaacaat atacacacag gtatttgaaa ttgataatgg catcgagaaa
     2641 agatgcatta atcactggca tacattatgt aatctgccag ccagcgaaca tgctgtttat
     2701 atttgtggtt ggcaagatat taccgagacg cgtgatttaa ttcatgcact cgaagtagag
     2761 agaaataaag cgatcaatgc aactgtcgca aaaagtcagt ttctggcaac aatgagtcac
     2821 gaaataagaa caccaataag ctccattatg ggcttcctgg aacttctgtc gggttctggt
     2881 cttagcaagg agcaacgggt ggaggcgatt tcactagcct acgccaccgg acaatcactc
     2941 ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt
     3001 caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc tttcggtgcg
     3061 attgctgcaa gcaaatcgat cgcattaagt tgcagcagta catttcctga tcattacctg
     3121 gttaagatcg accctcaggc gtttaagcag gtcttatcaa atttactgag taatgctctc
     3181 aaatttacca ccgagggggc agtaaaaatt acgacctccc tggttcacat tgatgacaac
     3241 cacgctgtaa tcaaaatgac gattatggat tctggaagtg gattatcaca ggaagaacaa
     3301 caacaactgt ttaaacgtta tagccaaaca agtgcaggtc gtcagcaaac aggttctggt
     3361 ttaggcttaa tgatctgcaa agaattaatt aaaaacatgc agggtgattt gtcattagaa
     3421 agtcatccag gcataggaac aacatttacg atcacaatcc cggtagaaat tatccaacaa
     3481 gtggcggctg tcgaggcaaa agcagaacaa cccatcacac tacctgaaaa gttgagcata
     3541 ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct aaatctatta
     3601 ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa
     3661 cattatgatc tgcttattac tgacgttaat atgccgaatg tggatggttt tgagttgact
     3721 cgcaaactcc gtgagcaaaa ttcgtcctta cccatctggg ggcttacagc caacgcacag
     3781 gctaacgaac gtgaaaaagg gttaaattgc ggcatgaact tatgtttgtt caaaccgttg
     3841 accctggatg tactgaaaac acatttaagt cagttacacc aggttgcgca tattgtacct
     3901 cagtatcgcc accttgatat cgaggccctg aaaaataata cggcgaacga tctacaactg
     3961 atgcaggaga ttctcatgac tttccagcat gaaacacata aagatttacc cgctgcgttt
     4021 catgcactag aagctggcga taatagaact ttccatcagt gtattcatcg catccacggt
     4081 gcggctaaca tcctgaattt gcaaaagttg attaatatta gccatcagtt agaaataaca
     4141 cctgtttcag atgacagtaa gcctgaaatt cttcagttgc taaactctgt aaaagaacac
     4201 attgcagagc tagaccagga aattgctgtt ttctgtcaac aaaataacta aatagtggct
     4261 cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat gatgagaact
     4321 cctggcgaat ttgctcccca tgctggtcga gcgttgccgc tcccggcata acatgcgggt
     4381 ccgcgcagcc actgattttt atcggattac ccggcatcat tattcccccg gcttcaatca
     4441 acatatttct cgcctgagtt tgtggcaaat taatggcctc agccacactt aataacggcg
     4501 cgacgggcac accaacttca tgtattctgg ctaaccaaac ttcagctgcc tgtgttttta
     4561 acgtccgctc aatatattgt ttaagaatag tctggttttg tacgcgtaaa atattgctgc
     4621 taaatctggg atcattaacc agttccgtaa gctccagtgc ctggcataac gcagagaaaa
     4681 gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag
     4741 gtgccatgta ggggtggcga tttcccaggc gttgtggtga cttccctgtc gcaatatatg
     4801 ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata tcgacatgcg
     4861 cccctctttg gctcttttcg cggccataaa gtgcactcac tattccgctg aataaataaa
     4921 caccgccgca tagatccgca agagaggtac caacacgcac tggcggagca tcagggtacc
     4981 ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca ggagcatctt
     5041 ttagcggacc ggtatgtccg aaacctgacg atgaagcata tatgaggcgc gggttgattt
     5101 cttgtaacct ttcccatgaa agccccagtt tttccattgt acctgggcga aaattctcag
     5161 ctaatacatc agcttgttta agcatattta taaatatact tttatcgtga tcattcttta
     5221 aatcaagaac cacactctct ttaccatgat taataaaact gtaatagagt gactgtccat
     5281 ccacataggg accaaatgtg cgggtatcat caccatgacc cggcggctca actttaatta
     5341 cccttgcgcc catattacaa agaagttgag ttccgaaagg tccattaagg acatgtgtca
     5401 tatcgataac taataagcct tcaaacggcc ctttgctttc attatttgtc atttgcttat
     5461 gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat caataacatg
     5521 attaattatg aaaccagtcg actgacataa atccacaagg gagccgtgac gacaaaaccc
     5581 agtacgctca cagccaatga cgctgtacca gtgcgggtgt agacattaaa ccggctggca
     5641 ataatgattc cggagaatgc cggcggtaat gcgcctgcca gtaccatcat ctgcagatgt
     5701 tcgctgttca aatgacatgc aataccaaca agaagcagcg ccagtggcat cagaatcagc
     5761 ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc agccagagtc
     5821 aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt aaaggttggg
     5881 tcccatgctg ccggaatttt taccccaacc aacaccagga tcgttgccag aacaggtgcc
     5941 catacaactg gctcctttgc cgcagaaatt aatgcgctca gattactatt cttcttacca
     6001 tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat tgcgttaaca
     6061 ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata aataggatcg
     6121 agaactgcaa accccaggaa tccaatggta ggtgaacctg caattaatgc acatacagct
     6181 gcttctgcat gggtacgttt aaaaaatttg tagcaaccaa accaggagaa aaagaaacat
     6241 ccgacaataa ccacgagtga tacaagggtc agacgagtgt ccgcaaaaat catttccctg
     6301 tttgcccggg taatagaaac aaatagggct gcaggaagcg cgtagtttaa taccagttta
     6361 ttaaatgccc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac
     6421 agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat aatagtattt
     6481 ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga tgtcagtatt
     6541 taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc agttcctgct
     6601 gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc ggtggttaaa
     6661 gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt
     6721 aatttgtcat accttgcatg gtgcaacaga tctgttggtg atggtgcacc agcgccactg
     6781 agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat cgttaccggc
     6841 aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc actatcacct
     6901 tcaatggcga caaccggaga accagaggtc acgctagcac cgatggcata gcccataccg
     6961 atgcccatga caccccaggt accacaatcc agacgacgac gtggtttata catatcaata
     7021 atatttcgtg cattatccag ggtatttgca ccttcattaa ctaaataaat atcctggttc
     7081 tcacgcaata catcgcgcac agcacttaat gcattaaagt aatttaatgg ttgggtatct
     7141 gtacttaatt tttcatgcat tttttgtgca ttttgctgct tgtggatatt taaaatatcg
     7201 cgccatacca gtggagtcgt aaatgtgttt tgtttcagct ctgccagcat accttgcata
     7261 ctggatgcaa tatcgccaac gactggcaca gcaatggggc ggttgctgtc aatttcctgc
     7321 ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt accgtgtgcc
     7381 agtaaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc cagggcaaac
     7441 gaacgcgcag ctgccgcaga aagtggatgc gtatcttcga ggatcccttt cgccatagac
     7501 attggcagga atggaatctg ggcactttca ataaattcac gaagctgttc gtcagcttgt
     7561 gaatacgccg cgcctttgcc aaggataatt aatggccgtt cagcttttgc taaaagcgaa
     7621 attgcgctag tgactgactt cgggcatggc aataatgctg gcgacggatt ttcaactttt
     7681 acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa
     7741 tcaagataaa ctccgccagg gcgacccgat actgagaccc ggatagcgcg tgccaatgca
     7801 atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg ttttgccgca
     7861 ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc gcggtcgctg
     7921 gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa
     7981 ccattgagga atcctggcgc agacactgtc aggcagatcc ctggtttttg ggtaagaaaa
     8041 ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat ataacgaatg
     8101 ccttccgcct gggcatggcg cgccatatcc gtcacaggaa tacctacaac accataaata
     8161 gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc tgtcatttga
     8221 agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc agaagccccc
     8281 gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca
     8341 gctcctgcaa taccgcagcg gtatgttcac ctaatagagg cgcagcttta atatccggcg
     8401 taaaggcaga gaatttcatt ggacaaccaa ctgtcagata ttttccacgc aatggttgtt
     8461 ccacttcgac aacactgccg ctttggcgta aagagggatc aagtgaaatt tctttcatac
     8521 ttaaaaccgg tgcacaagga atatcaaact gagtcaaata agccactgct tcatgtttat
     8581 caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg
     8641 catgggctgt actgtatgcc ggatcggtaa tccaatctgg tttgccgatg gctttacagg
     8701 tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga tcagtttccc
     8761 agcctttaca tttcaggatc cagccaggct gaccgccacc acccgcatta cctccgcggg
     8821 gaactgcatc accaaatgtg ccattcggat actgcgggta ttcttccaga taacccaatt
     8881 tatcgagacg ctgctggtcg cgtaatttca cgcggcaaag gttcaatacg gcatcctgca
     8941 ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta
     9001 aaccgatcag taaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg
     9061 gcggaccgtc ccaaaaaccc gtagtggatg ccgcgccacc cgctgcctga gcaacgtttt
     9121 cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa
     9181 tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatgtga tcaatggccc
     9241 ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc attacctctt
     9301 tgccttccgc tgttttggta tttaactcaa tagaacgttt gttactgtta agcatggtga
     9361 agtaaagcgc atcgatatca ggaatatctc gcagctggtg acgcgttacg tcaccaacgc
     9421 cggggcgttc aattttaatg acgtcagcgc caaaccaggc cagcatttga gtacaagatg
     9481 ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga gttgacatag
     9541 caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg cataacgccc
     9601 tctataaata atccgcaaaa tatcgtcata gcggcactcg atatttttaa attcctgtat
     9661 caagtgctga aaatcatttc atcgctactt ccaattcctt tctgcatggt aagtagtgag
     9721 gacgaaaaga agaatatgat aaatattggt tcgtggtaat cagagagagt cgcgacattt
     9781 gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaagg gtatgattac
     9841 gctttttaga gcgtaccata aaatataaca tattaaatga tttgtgatga cacgggatta
     9901 ttgcggttta ctttgaggaa tataaaatgc gagtttatag aacagcgagc gccaaataat
     9961 tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga gggagttaaa
    10021 tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt cgctatcgac
    10081 gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt gctttttatc
    10141 aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca tcagatattg
    10201 gttttccatt acaccaacac cggccaggcg aagctcttcc attgcgccct ttttatcccc
    10261 tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggtg tcgctacata
    10321 actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat tgacattggt
    10381 ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca acaaagctga
    10441 agcttcattg gtcagttctt ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc
    10501 tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg ctgcagctgc
    10561 cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg gagcattatc
    10621 agcagcccat acagttgaag atgccagaat agctgtgacc atcgtagcca taattaaacg
    10681 tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc
    10741 atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac agttgtagtt
    10801 attatcgcaa gaagtatttt agtcttacaa ttgtaggaat atgcttcctg
//
LOCUS       DAZGXS010000138        10804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGXS010000138 DAZGXS010000000
VERSION     DAZGXS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            54..737
                     /locus_tag="ACHKG5_003913"
     CDS             54..737
                     /locus_tag="ACHKG5_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869918.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            753..1163
                     /locus_tag="ACHKG5_003914"
     CDS             753..1163
                     /locus_tag="ACHKG5_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869919.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1384..2205
                     /locus_tag="ACHKG5_003915"
     CDS             1384..2205
                     /locus_tag="ACHKG5_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0869920.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGHSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2287..2766
                     /locus_tag="ACHKG5_003916"
     CDS             2287..2766
                     /locus_tag="ACHKG5_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0869921.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2781..3257
                     /gene="yeeS"
                     /locus_tag="ACHKG5_003917"
     CDS             2781..3257
                     /gene="yeeS"
                     /locus_tag="ACHKG5_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0869922.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3320..3541
                     /gene="yeeT"
                     /locus_tag="ACHKG5_003918"
     CDS             3320..3541
                     /gene="yeeT"
                     /locus_tag="ACHKG5_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0869923.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3615..3983
                     /gene="cbeA"
                     /locus_tag="ACHKG5_003919"
     CDS             3615..3983
                     /gene="cbeA"
                     /locus_tag="ACHKG5_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP0869924.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4072..4445
                     /gene="cbtA"
                     /locus_tag="ACHKG5_003920"
                     /pseudo
     CDS             4072..4445
                     /gene="cbtA"
                     /locus_tag="ACHKG5_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4442..4636
                     /gene="yeeW"
                     /locus_tag="ACHKG5_003921"
     CDS             4442..4636
                     /gene="yeeW"
                     /locus_tag="ACHKG5_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0869925.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4682..4762
                     /locus_tag="ACHKG5_003922"
                     /pseudo
     CDS             4682..4762
                     /locus_tag="ACHKG5_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5051..5197)
                     /locus_tag="ACHKG5_003923"
                     /pseudo
     CDS             complement(5051..5197)
                     /locus_tag="ACHKG5_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5251..5433
                     /locus_tag="ACHKG5_003924"
     CDS             5251..5433
                     /locus_tag="ACHKG5_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP0869926.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ACHKG5_003925"
     CDS             complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ACHKG5_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0869927.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ACHKG5_003926"
     CDS             complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ACHKG5_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0869928.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHKG5_003927"
     CDS             complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHKG5_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0869929.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGDWDIEMYVAVQPKHH"
     gene            complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHKG5_003928"
     CDS             complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHKG5_003928"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0869930.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            complement(9889..10046)
                     /locus_tag="ACHKG5_003929"
                     /pseudo
     CDS             complement(9889..10046)
                     /locus_tag="ACHKG5_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            10374..>10804
                     /gene="nleG7"
                     /locus_tag="ACHKG5_003930"
     CDS             10374..>10804
                     /gene="nleG7"
                     /locus_tag="ACHKG5_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFP0869931.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVP"
BASE COUNT         2893 a         2712 c         2471 g         2728 t
ORIGIN      
        1 ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta attatgaaat
       61 tacctgtaaa gttattaatg agccttatat ctctggtccg cgttattgca cgtgccgggg
      121 aatataaaaa ttacagccgg gatgaaataa aatactggcg atatacatca tacaaggggg
      181 ggaaacttcc ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa
      241 tatttacaat gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct
      301 ttaactttaa cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct
      361 gggctgaaca acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct
      421 acaaagccgc tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag
      481 gcggctatat ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct
      541 ggttccaggg cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct
      601 ataacccaaa aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct
      661 acctggtttg gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg
      721 tagctgcggt taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg
      781 ttgctgtaac tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata
      841 acatcaaata cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt
      901 gccagacaaa gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg
      961 gtggtgcctg gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac
     1021 aaatgattga atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa
     1081 tcaaaaatgg cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcggtgtag
     1141 cccaggacaa acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat
     1201 caaaggtaaa tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca
     1261 gtgaaaggcc tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc
     1321 gggagacgcg gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     1381 agtatgcgat tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     1441 ctgacacatg aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc
     1501 tcccgcagtg aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg
     1561 gaaggctttc agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     1621 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     1681 gaaattattc tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac
     1741 ttcaggttcg tctgccagaa cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt
     1801 ccacaccggg gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg
     1861 tttgaccgga tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gcaaccggca
     1921 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     1981 accaccgccg acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc
     2041 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtca cagtgcaaaa
     2101 ggaaaacgta tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc
     2161 gcattgtggg tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag
     2221 tgagggaatc attcagcttt cgttcccgaa aaacacacat ttatttgtta tttaaggagt
     2281 tatctcatga aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag
     2341 aataatccgc ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg
     2401 ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg agccccgtgt cttcggctgg
     2461 atggaccgtc tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     2521 ggcggggcat ttatggcccc cgaaccggat gatgatgatg acgaaacatg ggtactgttc
     2581 aatgccatga acggtaaccg cgctgaaatg agcccggaag ccgccggtat tgccgcctgt
     2641 ctgatgacgt acagccatca tgcctgtcgt acggagaatt atgccatgac ggtccattat
     2701 taccggttgc gggattacgc cctgcagcat ccggaaggca gcgccattat gcgcatcatt
     2761 gactgaaagg ggccggaata atgcaacaga tttcctttct gcccggagaa atgacgcccg
     2821 gcgagcgcag cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg
     2881 gtgtggcctt cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac
     2941 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg
     3001 aaaccctctt caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac
     3061 gcgccctgta ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag
     3121 tcacacccag taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg
     3181 tagatatccg ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg
     3241 aacatggtct gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg
     3301 tcttcagagg tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca
     3361 acaacatccg acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag
     3421 tgctgaagcg tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc
     3481 tgaacgccgt aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg
     3541 agtgggaatt ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct
     3601 gtaaggattt tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg
     3661 accgcccctg gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc
     3721 aggagggtaa ccggttgcat taccttgccg accgcgccgg tatcagaggc ctgttcagcg
     3781 atgcagatgc gtaccacccg gaccaggcct ttccgctgct gatgaaacaa ctggaactca
     3841 tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaag
     3901 ggctgacctg caaagccgat accctcagca gttgtggtta cgtttatctg gctgtttatc
     3961 cgacgcccga aatgaaaaat taactctcca gaatagcctt ctgctacggc ctggtgtttt
     4021 caccacgcca cttttccatt tttatatctg catatcagga aaatcttcag tatgaaaaca
     4081 ttacctgtat tacccgggca ggcggccagt tctcgcccgt ctcctgttga aatctggcag
     4141 atactgctgt cccgactgct ggaccagcac tatggcctca cactgaatga cacacctttt
     4201 gccgatgaac gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcggtg
     4261 aactttctcg tggaaaaata cgcgctggtg ctaccgacca gccgggattc agcgcctgta
     4321 cccgctctca gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga
     4381 tgacccgcga caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga
     4441 aatgatgaca ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt
     4501 cgttgacatt gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta
     4561 ttcactccgc gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc
     4621 tcaccagttg ctgtaagaca atgcagtgat gcagggtata gtgccattaa ccacagaaac
     4681 agcactgttg tcctgtatta agcaggcaag agaagatgcc cttaccctgc gccatctgca
     4741 gcttctgcac cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc
     4801 tgaatacagc tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga
     4861 cgccccgacc atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt
     4921 atggccgttt taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg
     4981 ggtaggaaca gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc
     5041 gtattgacgc ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt
     5101 tggagggcca ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggatcgatac
     5161 cgttcagacg gcaggtgcct gcttttccgg caacaagcag gaatctcagc gctgaagcag
     5221 gaattagagg taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc
     5281 aaagcagatt tagccagcat ggaacaaata tctttggtga aatgttggtt acgggaagct
     5341 ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt
     5401 gccttgctgc atacagaaga aggctgccgc taatttcgat aggttctcag atgagaatga
     5461 tgacggtgat acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg
     5521 aataaaacag gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt
     5581 tggagatatc gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc
     5641 gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc
     5701 ctggcttgat gtaatcgctc aggttgtcca gcaacaggac gcgcttctgg ttgtcacgga
     5761 tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa
     5821 caaattccag tacgtcctgg aatgaaggct tggtagtttc catttttata cccctgataa
     5881 tgtgagagtc ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct
     5941 gccgcagatg acaatgcttt tatcccttat cactatcata ccctttatcc ttgctgaatc
     6001 gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca
     6061 gactcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga
     6121 ttggtgtttc cacaaccttc aggtcatggt ggttattcag caactgacgc agctcttcca
     6181 cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg
     6241 catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg
     6301 cgttcaataa cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca
     6361 gcatacaaac cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac
     6421 gagtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt
     6481 tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat
     6541 agacccgatt atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc
     6601 gttgtggcca gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc
     6661 ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca
     6721 ccccaatcaa taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca
     6781 tcgccgccgc gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga
     6841 taagacctaa aatcgacccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa
     6901 cgttccccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc
     6961 cttccgggat agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc
     7021 gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact
     7081 tattggcacg cacgcagaaa ctccgttaat gaagattaaa agcgctcata acacatctgt
     7141 cggtcagaag gcgcgcctct gaattatccg acggacaaac cgggaacagt gtaatttccc
     7201 ggcaggagcg aaaaaattga aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg
     7261 tcttcgcgtc aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat
     7321 acatttcgat atcccagtcc ccatcttccg cgccattgtt caagtaaacc tcgaagcagg
     7381 gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg
     7441 gtttagcaaa atcatcaccg actacacgag ccaccgccac cgcatattga ccacctgaaa
     7501 tttctgtcag aatgacgccc tcactgtttt cggggagcgt aaagttattc ggtaccgtca
     7561 cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc cggattgtcg taatagacag
     7621 caacccactc cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaaac
     7681 ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttat
     7741 cttcctgctt aatctcgtag ttcatgacgc ctccattatt actgttttta tatacagtat
     7801 agttgcaata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
     7861 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
     7921 aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta
     7981 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagc
     8041 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc
     8101 atccagaact cttgttccgt tcccagggcg atattttctt tatcgccata ccagatgcgt
     8161 tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
     8221 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc
     8281 actgcaatga gacgacgctg cccatctaca gccgaagcaa tgagattaaa cccggcacca
     8341 gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taacccgtta
     8401 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
     8461 tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc
     8521 tgacctggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
     8581 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
     8641 agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
     8701 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac tggattatct
     8761 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
     8821 atagcgcgat ccacgacata gcccgtcatc agctttgtca ggctggcggg attgcgctgt
     8881 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggag
     8941 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
     9001 gataagttaa aaatgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
     9061 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
     9121 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgt
     9181 caaattgtcg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
     9241 aacaacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
     9301 gattacgcgc aacacccctg agagccgcca tgaacaaggg tttcataatt tgtgatttta
     9361 gtttggtacg caatttggta cacaacacaa ttttcacttc agtgggtagt catcaaactc
     9421 accataacga gcatcgttga tgatgtatgt tataaccaca aacatcacat ctggactagt
     9481 tccgttttca tgagcgggta tttgtttacg cctccctttc tgcaaatcct aaagatgaga
     9541 ttttggggtt ttgggatatc ttttatcctc agttctctat ccattatgca aaccagtaat
     9601 gaaccatcac agtgtgatag tgaggcgtct gccacgataa gcgcagagaa atgatgcaat
     9661 ggtgggcgga ctggcttgat gaaaaggtag agtgagcgac cttaaactat cgaatagaac
     9721 aaagtcttgc aatccagtgc aaagctttgt gcgcctcagt tttgtctaag cgttctactg
     9781 aaaacatagt aaaatcggta acggctggaa atcattcaat actcgcacta tcgaaagttc
     9841 gccagccagc cgtggcacgt tcttgcatac gacgtgccgc ggatcctatt attctagtat
     9901 gaaatcccta accgatttca aattctccag actgctcaga ttctctttta tcccacgctg
     9961 gcgctgataa atctcgtcat tgcaatcgac ctgcgctatc gctgccgcag ttcttccagt
    10021 ttctgcatcg ataactccat cttctgagca ataaacctca cctatgcaat attttatgtc
    10081 aattatttca attgcattaa tatcttgaat ggattacata gagttaatgt atccagtact
    10141 ccctattctc ttgcatataa tacatgttgc aacttacatc tcagcgctat gaaaaaacac
    10201 cacctctctc tttatgaaat tctggattta ccaagcgcta atttatcatt tcaatctacc
    10261 tttaagcatt gcatttatct ccctacgagg tcatacttta ggaagttaaa tatgaatgat
    10321 aatataccta cagcgcgaaa tcacaaacaa tcgacttgta ttacagaaaa aacatgccta
    10381 tctttttaaa cttcacagca ggcagtatct tacctgagaa tgagctagca tctttacgtt
    10441 atattgtgca gcaaaatcaa aatgatactg taatcataaa agaacgttat aaaatggata
    10501 tccgttatat cgaatcagtc aatggtttta cagtaaatcc tgtatgcagt aatcatttct
    10561 ccatatttat ggcgagacaa aacactattg ctcgcaacct ggaacagcag atcaacaacg
    10621 gacgaagttt tgcacaaata tctcaggatt ttatgcttca attatcttca aatataggat
    10681 ggaaaaaagg ggccgaaaac gcccttaaaa ataaaatcca ttctcattca tttgttgtaa
    10741 atcctgatga attctcttgc gacacacaat ttcttaagtg cccaataaca ttatgcgttc
    10801 caga
//
LOCUS       DAZGXS010000139        10604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGXS010000139 DAZGXS010000000
VERSION     DAZGXS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            110..1084
                     /gene="cysB"
                     /locus_tag="ACHKG5_003931"
     CDS             110..1084
                     /gene="cysB"
                     /locus_tag="ACHKG5_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0869932.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1403..1543
                     /gene="ymiA"
                     /locus_tag="ACHKG5_003932"
     CDS             1403..1543
                     /gene="ymiA"
                     /locus_tag="ACHKG5_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0869933.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1546..1713
                     /gene="yciX"
                     /locus_tag="ACHKG5_003933"
     CDS             1546..1713
                     /gene="yciX"
                     /locus_tag="ACHKG5_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0869934.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1827..1922
                     /gene="ymiC"
                     /locus_tag="ACHKG5_003934"
     CDS             1827..1922
                     /gene="ymiC"
                     /locus_tag="ACHKG5_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0869935.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2086..4761
                     /gene="acnA"
                     /locus_tag="ACHKG5_003935"
     CDS             2086..4761
                     /gene="acnA"
                     /locus_tag="ACHKG5_003935"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0869936.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4825..5415)
                     /gene="ribA"
                     /locus_tag="ACHKG5_003936"
     CDS             complement(4825..5415)
                     /gene="ribA"
                     /locus_tag="ACHKG5_003936"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0869937.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5585..6349
                     /gene="pgpB"
                     /locus_tag="ACHKG5_003937"
     CDS             5585..6349
                     /gene="pgpB"
                     /locus_tag="ACHKG5_003937"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0869938.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIQQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6498..6806
                     /gene="lapA"
                     /locus_tag="ACHKG5_003938"
     CDS             6498..6806
                     /gene="lapA"
                     /locus_tag="ACHKG5_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0869939.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            6813..7982
                     /gene="lapB"
                     /locus_tag="ACHKG5_003939"
     CDS             6813..7982
                     /gene="lapB"
                     /locus_tag="ACHKG5_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0869940.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8175..8912
                     /gene="pyrF"
                     /locus_tag="ACHKG5_003940"
     CDS             8175..8912
                     /gene="pyrF"
                     /locus_tag="ACHKG5_003940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0869941.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8912..9238
                     /gene="yciH"
                     /locus_tag="ACHKG5_003941"
     CDS             8912..9238
                     /gene="yciH"
                     /locus_tag="ACHKG5_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0869942.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9364..9582)
                     /gene="osmB"
                     /locus_tag="ACHKG5_003942"
     CDS             complement(9364..9582)
                     /gene="osmB"
                     /locus_tag="ACHKG5_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0869943.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9851..10600)
                     /gene="yciT"
                     /locus_tag="ACHKG5_003943"
     CDS             complement(9851..10600)
                     /gene="yciT"
                     /locus_tag="ACHKG5_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0869944.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2639 a         2471 c         2741 g         2753 t
ORIGIN      
        1 tgatatagtg gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag
       61 gtgtgacgaa aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca
      121 acaacttcgc tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga
      181 aggactttac acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct
      241 aggcattcaa attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcaggaca
      301 agaaataatt cgtatcgctc gcgaagtcct gtcgaaagtt gatgccataa aatcggttgc
      361 cggtgagcac acctggccgg ataaaggctc gctgtatatc gccaccacgc atacccaggc
      421 acgctacgcg ttaccaaacg tcatcaaagg ctttattgag cgttatcccc gcgtttcttt
      481 gcatatgcac cagggttcgc cgacacaaat tgctgatgcc gtctctaaag gtaatgcaga
      541 tttcgctatc gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta
      601 ccactggaat cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat
      661 taccattgaa gaactggcgc aatatccgtt ggtgacatat accttcggct ttactggacg
      721 ttcagaactg gatactgcct ttaatcgcgc agggttaacg ccgcgtatcg ttttcacggc
      781 aacggatgct gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc
      841 cagcatggcg gtggatccgg tcgccgatcc ggaccttgtg cgtgttgatg ctcacgatat
      901 cttcagccac agtacaacca aaattggttt tcgccgtagt acttttttgc gcagttatat
      961 gtatgatttc attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt
     1021 cgcattgcgc tccaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa
     1081 ataatttctt gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc
     1141 ttttgttact tatttgtata ccgtgcacaa tatttttatc cctcatattt atagggtaaa
     1201 ttacctgtaa agtagtgtct aattacccgc ctcgcaaatt ccagttttca aatatctatt
     1261 tcaggtcaaa agattgaatt attcactgga gacgattcgt aaattcgctg gatttttgac
     1321 taaagtttct ttaggatttg tctcatcgta tgattaatta caccaatcgt tcaacgtctt
     1381 gctgataagt gatggcgatt atatgaggtt agcaatgcct tctggaaatc aggaaccccg
     1441 ccgcgatcct gaattaaaac gtaaagcctg gctggcggtt tttcttggtt ctgcactttt
     1501 ctgggtggtt gtcgcactgc tgatttggaa agtgtgggga taactatggt tggtcaggag
     1561 caactggagt cgtcaccatt atgccagcat agtgacaatg aacccgaaac gaaacgggaa
     1621 tgttccgtcg ttattccaga cgactggcaa ctaacatcgc agcagcaagc ctttatagaa
     1681 ctgtttgctg aagatgatca gccgaaacaa taattatcat cattattatt acccattttt
     1741 aatgaattaa agggctttta atacaccgca gcaataacag gttgagttat ctcaacacaa
     1801 agtaataacc gttaagggtg tagcctatga tcaacacaaa tatgaaatat tggtcctgga
     1861 tgggtgcatt ttctctgtcg atgctcttct gggccgaact cctctggatc attactcact
     1921 gatccttgac cccgctgcgg cggggttgtc atttgctttg ccacaaggtt tctcctcttt
     1981 tatcaatttg ggttgttatc aaatcgttac gcgatgtttg tgttatcttt aatattcacc
     2041 ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc gtcaacccta
     2101 cgagaagcca gtaaggacgc gttgcaggcc aaagataaaa cttaccacta ctacagcctg
     2161 ccgcttgctg ccaaatcact gggcgatatc acccgtctac ccaagtcact caaagttttg
     2221 ctcgaaaatc tgctgcgctg gcaggatggt aactcggtta ccgaagagga tatccacgcg
     2281 ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg
     2341 gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc aatgcgcgaa
     2401 gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc ggtcgacctg
     2461 gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac
     2521 gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg gggaaagcaa
     2581 gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca tttgccatca ggttaacctc
     2641 gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat tgcttatccg
     2701 gatacactcg ttggtactga ctcgcacacc actatgatca acggccttgg cgtgctgggg
     2761 tggggcgtgg gtggcatcga agcagaagcc gcaatgttag gccagccggt ttccatgctt
     2821 atcccggacg tagtgggctt taaacttacc ggaaaattac gtgaaggtat taccgccaca
     2881 gacctggttc tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg gaaattcgtc
     2941 gaattttatg gtgatggtct ggattcacta ccgttggcgg atcgcgccac cattgccaat
     3001 atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgctgt aaccctcgat
     3061 tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa atatgccaaa
     3121 gcgcagggca tgtggcgtaa cccgggcgat gaaccgattt ttaccagtac gttagatctg
     3181 gatatgaatg acgttgaagc gagcctggca gggcctaaac gcccacagga tcgcgttgca
     3241 ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tggaagtgaa tgccacgcat
     3301 aaagatcgcc agccggtcga ttatgttatg aacggacatc agtatcagtt acctgatggc
     3361 gctgtggtca ttgctgcgat aacctcgtgc accaacactt ctaacccaag tgtgctgatg
     3421 gccgcaggct tgctggcgaa aaaagccgta actcttggcc tcaagcggca accgtgggtc
     3481 aaagcgtcgc tggcaccggg ttcgaaagtc gtttctgact atctggcaaa agcgaaactg
     3541 acaccgtatc tcgacgaact ggggtttaac cttgtgggat acggttgtac cacctgtatt
     3601 ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa tcaaaaaagg cgatttaacc
     3661 gtcggtgcag tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc gctggttaaa
     3721 actaactggc tggcctcgcc gccgctggtg gttgcctatg cgctggcggg aaatatgaat
     3781 atcaacctgg cttctgagcc tattggtcat gatcgtaaag gcgatccaat atatctgaaa
     3841 gatatctggc catcggcaca agaaattgcc cgtgcggtag aacaagtctc cacagaaatg
     3901 ttccgcaaag agtacgcaga agtctttgaa ggcactgcag agtggaaaga aattaacgtc
     3961 gcacgatccg atacctacgg atggcaggag gattcaacct atattcgttt atcgcctttc
     4021 tttgatgaaa tgcaggcaac gccagcacct gtggaagata ttcacggtgc gcggatcctc
     4081 gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag tattaagccc
     4141 gacagcccag cgggtcgata cctacaaggt cggggtgttg agcgaaaaga ctttaactcc
     4201 tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc caatattcgc
     4261 atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt acctgacagc
     4321 gacgtagtct ctatttatga tgctgcgatg cgctataagc aggagcaaac gccgctggcg
     4381 gtgattgccg ggaaagagta tggatcaggc tccagccgtg actgggcggc aaaaggtccg
     4441 cgtctgcttg gtattcgtgt ggtgattgcc gaatcgtttg aacgaattca ccgttcgaat
     4501 ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtaacgcg taaaacgtta
     4561 gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaatctaca acccggcgcg
     4621 acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc ctgccgttgt
     4681 cgtatcgaca ccgcgacgga gttgacttac taccagaacg acggcatttt gcattatgtc
     4741 attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt ccggcaagca
     4801 aattaattac acaatgcaag agggttattt gttcagcaaa tggcccattt tctcggcttt
     4861 ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta cgcgttcaac
     4921 aatattaatc cccgcttcgg tcagaatttc gacttttttc gggttattgg ttaacaagcg
     4981 gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt cgcgctcatc
     5041 agcggcgaag cctaactggt ggttagcctc tacagtatcg taaccttgat cctgcagtgc
     5101 gtaagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt gatacagcaa
     5161 aataccacgg ccttcctcgg caatttgcgt caatgccgct tcgagctgga agccacaatc
     5221 gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc gcgcaagtac
     5281 cggggtatgc ccggaaatat cgccatagac tagcgcgaca tgatcgtgtc cggttgccag
     5341 ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg cttctgccac
     5401 acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct tattttgcca
     5461 taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg gcacaaatct
     5521 gtcaattttt cctggaactg gcattttcag ttatgattgt gggacttatc aaaaaggaga
     5581 ggccatgcgt tcgattgcaa gacgtaccgc agtgggagct gcactattgc ttgttatgcc
     5641 agtagccgta tggatttctg gctggcgttg gcaacctgga gaacaaagtt ggctactaaa
     5701 agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta cacatttgat
     5761 tttattcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct ttgtattatt
     5821 tgccattctg gcggccgcaa tccttgtggg acaaggcgtt aaatcctgga tcaaagacaa
     5881 agtccaggaa ccacgacctt ttgttatctg gctggaaaaa acacatcata ttccggttga
     5941 tgagttctac actttaaagc gagcagaacg cggaaatcta gtgaaagaac agttggctga
     6001 agagaaaaat attcaacaat atttgcgttc acactggcag aaagagacgg ggtttgcctt
     6061 tccttccggt cacacgatgt ttgctgccag ttgggcactg ctggccgttg gtttgctgtg
     6121 gccgcgtcgg cgaacgttaa ccattgctat cttgctggtc tgggcaacgg gagtcatggg
     6181 aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta cgttgatttc
     6241 gtgggcactg gtggcggtgg caacgtggct tgcgcaacga atttgcgggc cattaacacc
     6301 acctgcggaa gaaaatcgcg aaatagcgca acgagaacaa gaaagttaac gctggttgat
     6361 tttccgaatt tagcccttaa atcatcaaca atgcgcgtgg atgccatttc gcagacggcg
     6421 cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt cgctctaacc
     6481 acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta gcgatcttcg
     6541 tgatttcggt cacgttgggt gcgcagaacg atcaacaggt gacgtttaat tatctgttag
     6601 cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgcg ggttttgcta
     6661 tcggttggtt gatttgtggc ctgttctggc tgcgagttcg tgtttccctg gtgcgcgctg
     6721 aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg gctgtagtgc
     6781 agcactcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt ctgcttttgc
     6841 ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa aacaagcaag
     6901 atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg cttagtaatc
     6961 aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggataca ggcaccgttg
     7021 aagcccacct tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat cgcgctattc
     7081 gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt ctgttggcga
     7141 ttcaacaact ggggcgtgat tacatggccg ccgggttata tgaccgcgcg gaagacatgt
     7201 tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag ttgctacaaa
     7261 tctaccaggc taccagtgag tggcagaaag caattgatgt tgccgaacgc ctggtgaagc
     7321 tgggtaaaga taaacagcgc gtcgaaattg cccatttcta ctgtgagtta gccctgcagc
     7381 atatggccag cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg gcggcggcag
     7441 ataaaaacag cgcccgcgta tccatcatga tgggacgcgt gtttatggcg aaaggagaat
     7501 acgccaaagc cgtcgaaagt ttgcaacgcg tgatatccca ggacagagaa ctggtcagcg
     7561 aaacgctgga aatgctgcaa acctgctatc agcagttggg taaaactgcc gaatgggcag
     7621 aattcctgca gcgcgcggtg gaagagaaca ccggtgccga tgctgaattg atgcttgctg
     7681 atatcatcga agcgcgcgac ggtagtgagg ccgcacaggt ctatattacg cgccagcttc
     7741 agcgtcatcc gaccatgcgt gtgttccata agttaatgga ttaccactta aatgaagcgg
     7801 aagaagggcg tgccaaagag agcctgatgg tgctgcgtga catggttggc gagaaggtac
     7861 gtagtaagcc tcgttatcgc tgccagaaat gtggttttac cgcatacacc ctctactggc
     7921 attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat tcgcggtctt gatggcctgt
     7981 aattttttaa aaaaaccgac tttagttaca acatactagt tatttatgtc ccattgtgct
     8041 ccggcaacgg cggcgcagaa aaagcctgcc aggggagaaa tcgcaactgt taatttttta
     8101 tttccacggg tagaatgctc gccgtttacc tgtttcgcgc cacttccggt gcccatcatc
     8161 aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt acgaattctc
     8221 ctgtggttgt tgcccttgat tatcataatc gtgatgccgc gctggccttt gtcgacaaga
     8281 tcgacccacg cgattgtcgt ctgaaggtcg gcaaagagat gtttacattg tttgggccac
     8341 agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg aaattccacg
     8401 atatccccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc gtgtggatgg
     8461 tgaatgttca tgcctctggt ggggcgcgta tgatgaccgc agcgcgtgag gcactggttc
     8521 cgtttggcaa agatgcaccg cttttgattg ctgtgacagt gttgaccagc atggaagcca
     8581 gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa cgtctggcag
     8641 cactgacgca aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa gctgtgcgct
     8701 ttaaacaggt attcggtcag gagttcaaac tggttacgcc gggcattcgt ccgcagggga
     8761 gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg gctggtgttg
     8821 attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcag acgctgaaag
     8881 cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc gtctggtcta
     8941 ctcaacagaa accgggcgta ttgatgagcc caaagcggcc ccagtacgct ctaaaggcga
     9001 tggtgtggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg tttgcctgat
     9061 taccggtatc gatctcgatg atgccgaact gacaaaactt gcagcggaac tgaagaaaaa
     9121 atgcggctgc ggcggagcag ttaaagatgg agttattgaa atccagggcg ataagcgtga
     9181 tttattaaaa tcactacttg aagcgaaagg gatgaaggta aaactcgcag gcggttaaca
     9241 taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat tattcagtta
     9301 tttcgtactg aacagaatca agcgtattat aattacgagt cgataatcga catttattta
     9361 cgattattta ccgacctggt gaccaataac accaccgaca gctgcgccac ctaatgtacc
     9421 caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg cgccgattgc
     9481 ggtgttgcgg tcccgtttag accagttaga acaggcactc agagacattg ccagggtaat
     9541 tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct ctcctgaatt
     9601 tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc ctggtgaaat
     9661 tattccgtga aatccgctcg cgcgcagggt tatatcacgc aggtgataat gacttcctgt
     9721 tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa tagtcttaat
     9781 tatcaaccag gaatcatctt agagcggatg atttgtcaaa ctgcaaatca tccgtagaga
     9841 agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt actgtgctca agatgcatca
     9901 gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca acgggaccga
     9961 tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc gcttcgcact
    10021 ctttttccag cacggcattc accacatcgg tacgcatcat atcgcgaccg gtgaatccag
    10081 tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc tgttggatgc
    10141 actggcgtgt caacgggcca accatacttt cgcttttttt ctggtacacg ccaccgagca
    10201 aaataacttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg accgtgatga
    10261 tagtgacatt tttcttctgc tcccccagag tccgggcgag cagggcattg ctgctgccat
    10321 tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg gcgagttcac
    10381 gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca agcgaaactg
    10441 caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga tcctggcgaa
    10501 tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc tgaccctggt
    10561 caatgaccat ctgtagaata gtttgttgtc gggaattcat cgct
//
LOCUS       DAZGXS010000140        10594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGXS010000140 DAZGXS010000000
VERSION     DAZGXS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(106..1107)
                     /locus_tag="ACHKG5_003944"
     CDS             complement(106..1107)
                     /locus_tag="ACHKG5_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0869945.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ACHKG5_003945"
     CDS             complement(1113..1460)
                     /locus_tag="ACHKG5_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFP0869946.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ACHKG5_003946"
     CDS             complement(1490..2140)
                     /locus_tag="ACHKG5_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869947.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ACHKG5_003947"
     CDS             complement(2156..2560)
                     /locus_tag="ACHKG5_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0869948.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2650..2787)
                     /locus_tag="ACHKG5_003948"
     CDS             complement(2650..2787)
                     /locus_tag="ACHKG5_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869949.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2859..3062
                     /locus_tag="ACHKG5_003949"
     CDS             2859..3062
                     /locus_tag="ACHKG5_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0869950.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3084..3434
                     /locus_tag="ACHKG5_003950"
     CDS             3084..3434
                     /locus_tag="ACHKG5_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0869951.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            3445..3723
                     /locus_tag="ACHKG5_003951"
     CDS             3445..3723
                     /locus_tag="ACHKG5_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869952.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            3735..3977
                     /locus_tag="ACHKG5_003952"
     CDS             3735..3977
                     /locus_tag="ACHKG5_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0869953.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3974..4087
                     /locus_tag="ACHKG5_003953"
     CDS             3974..4087
                     /locus_tag="ACHKG5_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869954.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            4180..4596
                     /locus_tag="ACHKG5_003954"
     CDS             4180..4596
                     /locus_tag="ACHKG5_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869955.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            4620..4823
                     /locus_tag="ACHKG5_003955"
     CDS             4620..4823
                     /locus_tag="ACHKG5_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869956.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            4820..5086
                     /locus_tag="ACHKG5_003956"
     CDS             4820..5086
                     /locus_tag="ACHKG5_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0869957.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            5083..5382
                     /locus_tag="ACHKG5_003957"
     CDS             5083..5382
                     /locus_tag="ACHKG5_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869958.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            5705..5935
                     /locus_tag="ACHKG5_003958"
     CDS             5705..5935
                     /locus_tag="ACHKG5_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0869959.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            6008..6373
                     /locus_tag="ACHKG5_003959"
     CDS             6008..6373
                     /locus_tag="ACHKG5_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869960.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            6380..9202
                     /locus_tag="ACHKG5_003960"
     CDS             6380..9202
                     /locus_tag="ACHKG5_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0869961.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            9279..10238
                     /locus_tag="ACHKG5_003961"
     CDS             9279..10238
                     /locus_tag="ACHKG5_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0869962.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            10243..10557
                     /locus_tag="ACHKG5_003962"
     CDS             10243..10557
                     /locus_tag="ACHKG5_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0869963.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2953 a         2837 c         2434 g         2370 t
ORIGIN      
        1 actatcaacc accatttata aatcataacc ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttgaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccaccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt ttaggaagca tagcttgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttctgcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtagcaaaca tatttttgtc aaactgagaa gcaaatggat ctcccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcac aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agccaaacct
     1261 gtaagtgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcagt attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacgccct agaaatgaca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg gacttctggt aaggtcgaac
     1621 tctctatgtg gcttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gccttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacggtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccaatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaattcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc cgaccaatat catttttcat acaaaaacct tgacaggtat
     2581 cttttttgat accaaaatga tttcacgagc tattagatga tcacaatata ccactatgaa
     2641 acaagttctt cacgatacca gatcacgcat tccgcgtaac accgccacag gtccaagact
     2701 ggcacttcgg ctgtccctcg aggagcgagc cgtcattgat gaaatggcag ctaaagaaca
     2761 acgctcatcc tctaacatgg cgcgcatgat ctcccttcgc ggcctagagc taacccagaa
     2821 agaacaaaac aaatcttcct gatcaggagg ctagtgggat gtcaggtata accatcaata
     2881 tcaatgtgaa tgccccctat gtatccctgc agaaatatgc agagataaca ggtatccctc
     2941 ttaatacatg caaaaagatg ttggctgacg gtcgaattat tatccgcccc aaacgcgcca
     3001 aaatggaaaa gcctgaagta aaccttgtgg cgatgttaaa agacgctttg gctaacagct
     3061 aaaacaatga acagagcacc atcatgaaaa aaaacgctaa taatccatac tccaaatttc
     3121 gtaatggcgt agaacgccat gtacaccacg tcgctaccag tgcatcacgt agtaacagtc
     3181 gctataacct gaacgagacg catgcaacac cggatggcca cgctgtaaaa caaatcggcg
     3241 aacatgcctg gctgattgag aaagctggaa tcgtggtcca caaatgccca cgcaatccgt
     3301 ttaccgggaa ccgcattttt gcattgaact gcggcgacaa tcacttcggg caggatttca
     3361 cattatacga agcacttcgc acggttgatc gtctgcttcg cgggcaaagt tttattaaac
     3421 aggctgattt ataacaggtg ctttatgacc aaagagcatg cacaaggtgt atttatccgt
     3481 tttattgatt ttcgcggtga actgttatta cgcgcatcag ccattgacgg aattgttcca
     3541 tccgaaaaaa acgcagctac ttacgtttat ctgaacggta cgcgcctgac tgtggagctt
     3601 ccgtaccaga ccgtacgaga aatcattagc gaagctgaaa aggctcgtca ggttaatggc
     3661 gatgaaccct atatcgaaat tatttgtatg gattcagaag ctgaaattca gaaagcagat
     3721 taaagggcgt tgcgatgggc aaagaatata aaactctcat taacaaagca cctgaacgat
     3781 tttattttcg cttaagtgca tcaggcgctc atgctgaacg tgcagcccgt gactcattga
     3841 ccagggcaat ccggagtttg tatgacgttg ctttttacgc tgatgatctg gatgcactta
     3901 acgaactttc cgagctgatc tgtgccgcag aatgcgggga acacattgaa ccatataagc
     3961 tggggaatat cgcatgagta tatttatctc atggcttgtt ctgattattt cggtggcctg
     4021 cgccattggg attatgcgaa ttattcattc agtaaaaaag attgaacgct ttttcactga
     4081 caaataacag cttaaacaga acaccagatt aaacccgaaa acctgaaaac tatccgcatt
     4141 cgcggaggta ttcgcacacg taaataacgg agatataaaa tgaacgcaaa agaaaaaaat
     4201 attatcaata cattaaaaat agtgtcagca gagcaagaca agctgtccag ggcagcccag
     4261 aaagataatc aacatatggc agcgctttac gcactgacca tcgcaatagc cacccctgaa
     4321 gcagccaaag ttattgagga acagagcaaa gaaatcgaca ctcttaaaac acagtcaaca
     4381 gttgcagcca tgaatccgtc cagcattgga cgctgcattt atattcttgg ttcagcaatg
     4441 atgctgcaat acaccattat tgccgaactg cacggcaaat acctgataac gccttaccac
     4501 acaaaagagt cagagcttct gacaaatctc cgcctgatag aacgctctca agctgtattc
     4561 attgatgacg cgcaacgtgc cgtatttaac gcatagggtt actggacaaa gggggcgcaa
     4621 tggcaattaa gcattttccc gtcgttcgct ttacctccag agggcgcgaa tacgaggtcg
     4681 acgaacgcct gattaccact atcgacaaac atcgttcgga aaaggatgca caccacatct
     4741 acctcactga cggcacttac ttctgcgcca ccaacgtggc gcgagtaaat cttatccgac
     4801 aggtacagga gccacgcaga tgaccattct ggactacatc gctactcatc cggggtgtag
     4861 cggcggagag atcgccgcag cactgaatac cccaaccaca gccattaatg ctgagttacg
     4921 ccgactttgg cgcggcggct tagtcatcag aacaaaccgc agcacaggtg gtcgcgctcg
     4981 caaaacagga ggccaggctt cttaccacgt aaacccgatg ccgttcgggt gtagcaatcc
     5041 acttactcac atgtttaacc agctactgaa ggaagccaga gcatgagcac catcaaccac
     5101 cagaagctac gcgaactggc atttgccctg caacgaatgg caacgcctca aaaattactg
     5161 gcatttcgcg caatgctctc gccgtctgct gtgctggcac tgctggatca gctggagcac
     5221 gccagaacca cggctcctgc cattcgcctc acgctccatc atgaaatcgc tgatttctgc
     5281 gcaacgctgg gggcacctgg cgaaccggaa acgccggaag caatgcagca agagctgctg
     5341 caacgcatcg ataacgtttt tgattttttt ctgaaccagt aagaaaccag aacatgcaca
     5401 cacaaaaaaa ccgcttgcca tgccgcaatc ggtcaggtta catttccgct gcacctcata
     5461 aaacgggtgc cgggattctc aacccgatac agagcaaagc gcataaccgc gctagcggtt
     5521 tttttgtgcg tactgtattg ccacgtcttt ttcgcgtcag aattatggcg gggcgtacgg
     5581 ggccgacttc ggtcgggccg gattctttgc tctccggtgt tgagaaccct gtacgtctcg
     5641 ccaccccgag attctcaact ctggatggtg agttatttct atcaccgagc aaagaggcca
     5701 caccatggca gaccgcaaac agcaccgcgc tatcgcggag cgtcgtcaca tccagactga
     5761 aatcaaccgc agactttccc gcgcatcacg cgtcgcgcaa atcatgcaca tcaatatgct
     5821 gcatgagcgc agccacgcac tatcaaacat ttattccgcc tctgttttca gctatctggc
     5881 ggatgatctg cacgagtttc aacagctcat ccagcagcaa aacaaactcc attaattcct
     5941 gttccgggcc tttcctgcac cttgcggcgg gaggccttcg cacatctgta acaagaggat
     6001 tgccgcaatg attctcgcca acgactttct tgaatacctg ctcaacacag aacgtgatct
     6061 tgccgctcgc gtgcgtgatc gttatgacat gtacctgaaa tccctgcctg taccgcagct
     6121 cgctgacgga aagattgtta ttgatggtcg ctacatgatt gacagccacg agggaaatta
     6181 caggctttac cgcattgaag gtggcacccc gtccgttatt ggcatttacc agcgcccatc
     6241 ctctgcaatc gtcgatgtga ttgccgacag catccgcatc acacatcgcc atgccgacac
     6301 agaagacacc gtgctggaaa ttcagcggct ggctacagcc tgccgcgaca ccctgaatgg
     6361 catgacgaag taaatcacta tgacggcaga gtacatcagg gactggcaac aaccgcgcca
     6421 cgcagtgggg cgtgaaggaa cggggatccc cgctcctgaa tccgcgcttt cctcctggct
     6481 ggatgcctac cgggtagaga acgagcgccg ccaggaaatg gctgatgcgg cgttctccgc
     6541 aacgccgctg ggcaacctga ttaataaaag cctggacgca caggaaaaac aggacaaaac
     6601 catcacactg gcaggagacg ccagaaaaca ggcacgcggt gcggtggatg aagccatggc
     6661 ctcgctgcgc ctgctgccgt cctatctgcg cgatccgctt attcgccacc tctccttcct
     6721 gcgcaaaaaa caggaagccg atcgtcagaa aggcaaaaag agctggcagg ctgaacgcta
     6781 cgcgcgcgga aacctgcgca aaatattcga acgtctggag cgcaccgatc accgctggct
     6841 gacacagggt tatcgctccc ttgccggacg cgaacgcctg gacgatttgc tttacctgcc
     6901 gcagctcaac aaacaccaga tacagacgct ggccaccatg acggcggcga tgttcagcag
     6961 caccttcgaa aaactctgcg atggctttgg cgcgaccgat ggcgaactga ccatggatgt
     7021 aacgctgaag gcgtatcaga tgctggcccg catggcgtta cacctgcacg ccatgcctcc
     7081 acattatgac gcactgacaa cagacaaaga ccggaggaac gaaccggaca cggagctgct
     7141 gccgggcgca atccttcgcc tgacctgtgc ggaatggtgg aaacgcaaac tgtggctgtt
     7201 acgttgcgag tggagagaag aacaactccg cgccgcctgt ctggtttcca gaaaaacatc
     7261 gccctatctg agccaggacg cgttaagcga gtttcgcgca cagcgcgaga aaacacgcga
     7321 tttcctgaaa agtttcatgc tggaaaacga agacgggttc acgattgatc tcgagacagt
     7381 gtattacgcg ggagtaagta acccggttca ccgtaaggca gaaatgatgg ccaccatgaa
     7441 ggggctggaa cttctggccg aagcccgtgg cgacaaagcg gtgtttctga ctgtcacctg
     7501 cccgtcaaaa taccacgcta caacagagaa cggtcatccg aatcccaaat ggaacggggc
     7561 caccatgcgc gactccagcg attacctggt taacacgttt tttgcggcgg tccgcaagaa
     7621 actgaaccgc gacggcctgc gctggtatgg catccgcacg gtggagcctc accatgacgg
     7681 caccgtgcac tggcatatga tggtctttgc tcatccggaa gaaatcgaca ccattgtgtc
     7741 ccacacccgc gatattgcca ttcaggaaga tcgtcacgag ctgggcgatg atattactcc
     7801 gcgctttaag gcggagtatg tcgacggctc aaaaggcacg ccaaccagct acatcgccac
     7861 ctacatcgga aaaaacctgg acagccgcgc cgtggatggc atcgacccga aaacaggcaa
     7921 accacgcgtt gaccacgaaa ccggaaaatc aatgaccgag agcgtggaac gcgccattgg
     7981 ctgggcgcgc cttcaccggg tccgccagtt ccagttcttt ggcatcccct cccgtcaggt
     8041 gtggcgtgaa ctgcgtcgcc ttgccagcca gatggcacgc aacccggaag gcccgcaacg
     8101 gctgaaggat gacgcaatgg atgcggttct tgctgccgct gatgccggat gttttaccac
     8161 ctacattgag aaacagggag gcgtacttgt tccacgcaag gactacctga ttcgcaccgc
     8221 ctacgacctc gcagatgagc tgaacgatta cggcgaacag agcgtacaga tttacgggat
     8281 ctggtcacca ctcatcgggg aatcctcccg tgtgtgcacg cacccggata actggaagct
     8341 ggtaagacgt aaaccgggag tagaagacag cgcccgcgaa aatggttttg accttcaggg
     8401 cggccctgcc gccccttgga ctcgtggcaa taactgtccc cgtgtacagg aaacggacaa
     8461 caacgggaca gaacagccgg aagaacggcc agcaccgtgg ccgcagcttc ctgacggcgt
     8521 tgacgtgaac gaatggatgc gctcactgaa acggcacgaa cgccgggcgc tgatgcgttc
     8581 gcttcgtgac aaacaggcaa aaaacagcag tgatgaaatg cagagctgga cacagagccg
     8641 caaacagcag cggcctttgc ctgataacca cgaattactc gctaaagaat ggcgggagtc
     8701 tgctgaatct ctcggcctgc atatcggtga acaacagatg cagcacctgt tacggggcgg
     8761 cagtctgtac gttgacggca gcatcattgc accgcaggga tttgaaattg tacgcaaacc
     8821 ggatacccgc ccggacagcc gaatcacgca gctctggcag cgcctgagcc gtaatcatgg
     8881 cgtaagcagc acggagatcc gccataaccc ggtcgccagc tatctggcac agctgggggc
     8941 atcagaccct gaagccgccg cacgcctggc atccacactt cagcaggacc agaacaccat
     9001 gaaaacaccc gttaccgtgc tttctgacat gctgcgcgcc atccgcgacg cagagcacgc
     9061 acagagaatc agtgaaacca ctgaacgcgc cagccgcaaa gcagacctgc tgcggggtgg
     9121 cctgaccagt ggaaacaaaa aacagacaga aacgggactc acaaatcccg taaatgagca
     9181 aaaaacgcgc cgcgatatat gaagcgcgca caaaacaggc aaaagcggga tttaaaaatc
     9241 ctgtaaccga ttaattaatc aacataagga aaagcgacat gaaaatttgt atcgacgacg
     9301 gctccaccaa catcaagctg gcatggactg agaacggcga acgccgcaac gccatcagcc
     9361 cgaacagctt caagtcggaa tggtctgcgc cattcggtgg cacgcagccc gcgaactaca
     9421 tgcttgatgg cgtgcgctat ggttttgatc cggtcagcga tcgctttgtc cagacgaccg
     9481 acacgcaata ccagtacagc gatgtgaatg taattgccat tcatcacgcg ctggtcaaat
     9541 caggcatcac accacaggaa gtggatgtgg ttgtcaccct gccactgagc gagtatttcg
     9601 atacaaacgc acagccggac atggccaaca tcaaccgcaa aaaagcgaac gtcatgcgcc
     9661 cggtggagta ccagaacggc gaagcattca ctatccgtaa cgtacgggtt atgcctgaat
     9721 ccattcctgc tggctttaag gcactggctg acatgagtcc gtttgaatcc ctgctgattg
     9781 tggatttggg cggaaccacg ctggatgtgg caaaggttca ggggcaactg gcaggtatca
     9841 gccaggtgtt ttgcgatcca cacgtaggcg tttctctgat ggccgatgcc gtactgtcgg
     9901 tgatggccac taacggtatg cgcaccagtc accacatcgc caataccatt atcgaacatc
     9961 gccatgatga agcctggctg cgccagcaca tccacaatga cgcgcattac gccagcctga
    10021 tggcggttat tcgtgaaaag gaagaaacac tgaaacaacg cgtgatccgc gcgctggcgg
    10081 ttttttcggg ttacgggcgg gtgatggttg tcggtggcgg ggcggagatt gtggcaaccg
    10141 ctatccgcga agcctgcgga gttaatgcga ctttcatcgc ggacggggtg ccacagtttg
    10201 ctctggttaa tgggttgtac gcaatgaaca aggagtaaac caatgacgac accaaccaga
    10261 cggataagtt tctatctgaa gcccgccgcc gtcaagagtg aacaggaggc gtgcaattac
    10321 ctcgatagcc tgccagcctc cgaacgcagc cgcgcgcaac gcgcggcctt tctggctggg
    10381 ctagcactca taaagcgcaa cccagcattt gcctactgga tggccgaatg gccagaggac
    10441 aacctcccct tcaataccgt tagcatcaag gaaacatacc aggctaatca gcctagcgga
    10501 gttgaatgca atttttataa aataaaaaag aatattcaga ccttattccc agaataatct
    10561 gataattgta tgtgaactaa taaaatacat acca
//
LOCUS       DAZGXS010000141        10579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGXS010000141 DAZGXS010000000
VERSION     DAZGXS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            31..1083
                     /locus_tag="ACHKG5_003963"
     CDS             31..1083
                     /locus_tag="ACHKG5_003963"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP0869964.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1159..2592
                     /locus_tag="ACHKG5_003964"
     CDS             1159..2592
                     /locus_tag="ACHKG5_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0869965.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2775..5036
                     /locus_tag="ACHKG5_003965"
     CDS             2775..5036
                     /locus_tag="ACHKG5_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0869966.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5177..6460)
                     /locus_tag="ACHKG5_003966"
     CDS             complement(5177..6460)
                     /locus_tag="ACHKG5_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0869967.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRKLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6595..7665)
                     /locus_tag="ACHKG5_003967"
     CDS             complement(6595..7665)
                     /locus_tag="ACHKG5_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0869968.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7643..7861)
                     /locus_tag="ACHKG5_003968"
     CDS             complement(7643..7861)
                     /locus_tag="ACHKG5_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0869969.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(7901..8068)
                     /locus_tag="ACHKG5_003969"
     CDS             complement(7901..8068)
                     /locus_tag="ACHKG5_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869970.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            8311..8913
                     /locus_tag="ACHKG5_003970"
     CDS             8311..8913
                     /locus_tag="ACHKG5_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869971.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(9124..9345)
                     /locus_tag="ACHKG5_003971"
     CDS             complement(9124..9345)
                     /locus_tag="ACHKG5_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0869972.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(9444..9659)
                     /locus_tag="ACHKG5_003972"
     CDS             complement(9444..9659)
                     /locus_tag="ACHKG5_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0869973.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(9736..9927)
                     /locus_tag="ACHKG5_003973"
     CDS             complement(9736..9927)
                     /locus_tag="ACHKG5_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0869974.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(9900..10082)
                     /locus_tag="ACHKG5_003974"
     CDS             complement(9900..10082)
                     /locus_tag="ACHKG5_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP0869975.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(10079..>10579)
                     /locus_tag="ACHKG5_003975"
     CDS             complement(10079..>10579)
                     /locus_tag="ACHKG5_003975"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0869976.1"
                     /translation="LLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSM
                     WVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIGFV
                     FGEQWR"
BASE COUNT         2526 a         2507 c         2594 g         2952 t
ORIGIN      
        1 aagttatgtt tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga
       61 ggtggaacct cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag
      121 cgcgataaaa tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt
      181 ggcggcggta atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt
      241 gctgatgttg attatctgtt tgctcaagtt atcgtccatg agaaacgtgt cgataccacg
      301 cctaactgcg gcaatatgct gtctggcgtt ggggcattcg ccattgaaaa tggtttgatt
      361 gcagcgactt cgccagttac ccgcgtacgt atccgcaacg tcaatacggg tacgttcatc
      421 gaagctgatg tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac
      481 ggcgtaccgg gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa
      541 accggaaaag ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc
      601 tgtatcgata tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggc
      661 tatgaattac cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc
      721 cgtctacaag cgggtaaagc aatgggctta ggcgatgtca gtaatatggt tatccctaaa
      781 cctgtgctta tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg
      841 cattcttgcc atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca
      901 ttggaaggta ccgtcacccg acaaatcgtc ccttctgtag gatacggcaa tatcaatatt
      961 gaacacccca gtggtgggct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg
     1021 ttacgcgcat ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc
     1081 tgaaaagatt cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca
     1141 gaaagaacag gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg
     1201 atcccatgta ttattggctt tatgccagct ccggcaggat taagcgaact ggcgtgggtg
     1261 ctttttggta tttacctggc ggccattgtg gggctggtta tcaagccttt cccggaacct
     1321 gtcgtactgt taattgccgt tgctgcctcc atggtggtgg tcggtaactt atccgacggt
     1381 gcgtttaaga ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc
     1441 tcggcgttta ccttaagcgc cgcatttgtg accaccggtt taggtaaacg tattgcctat
     1501 ctgctgattg gtaaaatcgg taacaccacg ctgggtctgg gttacgttac ggtattcctc
     1561 gatctggtat tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta
     1621 ccgatcatca acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt
     1681 gtcggacatt acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc
     1741 tttaccgcaa tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg
     1801 caaattagct ggggtggatg ggcgctggcc gccggattgc cgggcatcat tatgctgctg
     1861 gtcaccccgc tggtgattta caccatgtat ccaccagaaa ttaagaaggt ggataacaaa
     1921 accatcgcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg
     1981 ctcggtgtct ttgtgctggc gctgctgggc tggattttca gtaagtctct gggggttgat
     2041 gaatcttcag tggctattgt tgttatggcg accatgctgc tgctgggtat cgttacctgg
     2101 gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc
     2161 ggcttaagct ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa
     2221 aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc
     2281 atcatcgtgc gttatttctt cgcttccggt agtgcctata tcgttgctat gttaccggta
     2341 tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg
     2401 ttctccaact cctatggcgg catggttact cactatggcg gcgcggcagg tccggtcatc
     2461 tttggcgtgg gttataacga tattaaatcc tggtggttgg tcggtgcggt actgacgata
     2521 ttaaccttcc tggtgcatat caccctcggc gtgtggtggt ggaatatgct gatcggctgg
     2581 aacatgctgt aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc
     2641 accccagtca cttacttatg taagctcctg gggattcact cgcttgtcgc cttcctgcaa
     2701 ctcgaattat ttagagtata tccatttatt ttcttgctgc gcacttcacg gtgcgcagat
     2761 atctggagca tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc agtaataacg
     2821 aaataattgc cgaagaacat ttcaccggcg aaattaaaaa agaagaagca aaaaaaggca
     2881 ctattgcctg gtctattctc tcttctcata atacgtccgg aaatatggat aaacttaaaa
     2941 ttaagtttga ttcattagcc tctcacgata ttacctttgt tggtattgta cagaccgcta
     3001 aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct gaccaactgc cataactcac
     3061 tctgcgccgt cggcggcacc attaacggtg atgaccatgt ttttggttta tcggcagctc
     3121 agcgttatgg cggtattttt gtgcctccgc atattgcggt catccatcaa tatatgcgtg
     3181 agatgatggc aggtggcggc aaaatgatcc tcgggtcaga cagtcacacc cgttacggtg
     3241 cattagggac aatggcagtc ggtgagggcg gcggcgagtt ggtaaaacaa ttgcttaatg
     3301 acacctggga tatcgactat ccgggagttg ttgcggtgca tctgaccgga aaaccagcgc
     3361 cgtatgtggg gccgcaggat gtggcgttgg ctatcatcgg tgccgtgttc aaaaacggct
     3421 acgtcaaaaa caaagtgatg gaattcgtag gtcccggtgt tgctgcgctc tctaccgatt
     3481 tccgtaacag cgttgacgtt atgaccactg aaacgacctg tttaagttct gtctggcaaa
     3541 ccgatgaaga agtccataac tggctggcgc tgcacggtcg cggccaggat tactgccagc
     3601 ttaaccctca accgatggcg tactacgatg gctgcatcag cgttgattta agcgccatca
     3661 aaccaatgat tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac acactgaacc
     3721 agaacttgac cgacattctg cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta
     3781 aagccaaact ctcgctgctg gacaaagtgg aaaatggtcg cctgaaagtg cagcagggga
     3841 ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc
     3901 gcggtcaatc ctgtggcaat gacaccttct cgctggcggt ttacccgtca tcacagccgg
     3961 tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt gattggcgca ggcgcaatca
     4021 tcagaaccgc gttctgcggc ccatgctttg gcgcgggcga tacgccaatc aacaacggtt
     4081 tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga aggctctaag ccagctaatg
     4141 ggcagatgtc agcggtggcg ttgatggacg ctcgttctat cgctgcgact gcggcaaacg
     4201 gtggctattt aacctctgcc agcgaactcg attgctggga caacgtgccg gagtacgcct
     4261 tcgatgtaac gccgtataaa aatcgcgtct atcaaggctt tgtgaaaggg gcggcccagc
     4321 aaccgctgat ttacggaccg aacattaaag actggccgga attgggtgcg ctgactgaca
     4381 atatcgtcct caaagtgtgc tcgaagatcc tcgacgaagt gaccaccacc gacgaattga
     4441 ttccttccgg tgaaacctct tcttatcgtt caaatccgat tggtctggcg gagtttaccc
     4501 tgtcacgccg cgatccaggt tatgttggca gaagtaaagc gactgctgag ctggaaaatc
     4561 agcgtctggc ggggaatgtc agcgagctga cagaggtgtt tgcgcgcatt aagcagattg
     4621 ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg cagcatggtc tatgcggtga
     4681 aaccaggcga tggttctgcg cgtgaacagg cggcgagttg tcagcgtgtg attggcggtc
     4741 tggcgaatat tgccgaggag tacgcgacta aacgctatcg ttctaacgtc atcaactggg
     4801 ggatgttacc gctgcagatg gtggaagcgc caacctttga agtgggggat tacatttaca
     4861 tccctggcat taaagcggcg ctggataatc cgggtacgac gtttaaaggt tatgtgatcc
     4921 atgaagatgc gccggtaacg gaaattacgc tctatatgga aagtctgact gctgaagagc
     4981 gcgagattat caaggcgggt agtttgatta acttcaataa aaaccgtcag atgtaaaaag
     5041 cgccatgtga atgtaggtcg cattcggcac ttattgtcgg atgcgatgct ggcgcatctt
     5101 atccgaccta caaatcgcat cgaaccgtag gccggataag gcgtttacgc cgcatccggc
     5161 aaatagttaa tagctcttac ttcttcgcct ctgcaaccac tttgctaccc acgccgcggt
     5221 tattgtattc ccacatgcgg ttgtagttag tgtcattcag cttgcgctgt atttcgtcgc
     5281 tatcgtcaac gctgccggtg ttacccgcaa acggacgatt agagatcacc gcatcgcccc
     5341 acggtttagc cgtgttaaaa ccttcgttga tggcgctatc acggatcacc acctgaccgt
     5401 tggtattggc atcaacatcc agcgagcggc ccagttgtgc cacgccatca ccggaagcat
     5461 tgaaacggct gtttacggcg aggaaaccgt agtaaatgtt ggacagcgta gccggtgcaa
     5521 acacatacgc ttcttgctga gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca
     5581 ccactgcgcc gcgaccagaa acgatatcca catccccttc aatgtagctg ttggtcacca
     5641 gcgtacgcgg ctgacgattc gtttccagac ggttctgcac accgctgttg gtgacaaaga
     5701 aggtgttctg acgaccgaga atgttaacgt tgttaatctg tacctggtca ccgtcagtac
     5761 gcagtgccac cgccggatgg ttacccgcat ctacgctatc acccagcgtg ttttcgatgg
     5821 tcaggttttg cagttgcagg ccattgtttt gtgaccagaa gaccgcagaa cacagaacac
     5881 cgatactgtc gctgcgtttg ctctggcagc tatcgtacat ataccacgct ggtttacctg
     5941 gcatatattt gccgcgcggg ttgacgtcgt gacgccagtc ggcagggctc atgcccccat
     6001 caagggaaag cccaatcttc acatcaatcg gtttttcacc tgtaccgtac agagtaattc
     6061 cacctggagc agcagggaca tacaccgttc cctgatactc accaggcatc acggcaatat
     6121 actggcgctt gttggtgcgc ttgataattg ccgcatctac cgccgcctga atcgtggtat
     6181 gcgttacacc ttgagtaccc gccgggccga caacaaagtc aggttgcgca ggcagggtta
     6241 tcggggacgg attccacgct gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat
     6301 cgaaattctg cgcttctttt gccgacagaa tcgggcgaga agaggtacca ggcgcagttt
     6361 gatcagaagg acgttgatcg ggtggtgttg agctacaggc ggtcagcgtc acgccaaaag
     6421 ccaatgccag cgccagacgg gaaactgaaa atgtgttcac aggttgctcc gggctatgaa
     6481 atagaaaaat gaatccgttg aagtctgctt ttttatacta agttggcatt ataaaaaagc
     6541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     6601 gatttcaatt ttgtcccact ccctgcctct gtcatcacga tactgtgatg ccatggtgtc
     6661 cgacttatgc ccgagaagat gttgagcaaa cttatcgctt atctgcttct catagagtct
     6721 tgcagacaaa ctgcgcaact cgtgaaaggt aggcggatcc ccttcgaagg aaagacctga
     6781 tgcttttcgt gcgcgcataa aataccttga tactgtgccg gatgaaagcg gttcacgacg
     6841 agtagatgca attatggttt ctccgccaag aatctctttg catttatcaa gtgtttcctt
     6901 cattgatatc ccgagagcat caacatgcaa tgttgtaggg atggcaattt ttacgcctgt
     6961 tttgctttgt tcgacataaa gatatccatc tacgatatca gaccacttca tttcgcataa
     7021 atcaccaact cgctgcccgg taacaacagc cagttccatt gcaagtctga gccaacatgg
     7081 tgatgattct gctgcttgat aaattttcag gtattcgtca gccgtaagtc ttgatctcct
     7141 tacctctgat tttgctgcgc gagtggcagc gaccgggttt gttgttatat ggccttcagc
     7201 tattgcctct cggaatgcat cgctcagtgt tgatctgatt aacttggctg acgccgcctt
     7261 gccctcgtct atgtatccat tgagcattgc cgcaatttct tttgtggtga tgtcttcaag
     7321 tggagcatca ggcagccccc tccttattgc tttaattttg ctcatgtaat ttatgagtgt
     7381 cttctgcttg attcctctgc tggcgaggat tttttcgtag cgatcaagcc atgaatgtaa
     7441 cgtaacagaa ttatcactgt tgattctcgc tgtcagaggc ttgtgtttgt gtcctgaaaa
     7501 taactcaata ttggcctgta ttgcttcagt gattgctatc ctcctgtctc ggcctaaacc
     7561 aaactcttta cccgtccttg ggtccctgta gcagtaatat ccattgtttc ttatataaag
     7621 gttagggggt aaatcccggc gctcatgact tcgccttctt cccatttctg atcctcttca
     7681 aaaggctacc tgttactggt cgatttaagt caacctttac cgctgattcg tggaacagat
     7741 actctcttcc atccttaacc ggaggaggga atatcctgca ttcgcgcacc catcgacgaa
     7801 ctgtttcaag gcttcttggg cgtcgctggc gtgcgttcca ctcctgaagt gtcaagtaca
     7861 tcgcaaagtc tccgcaatta cacgcaagaa aaagccgcca tcaggcggct tggtgttctt
     7921 tcagttcttc aattcgaata ttggttatgt ctgcatgtgc tatctgcgcc catatcatcc
     7981 agtggtcata gcagtcattg atgttctctg cttcgataac tctgttgaat ggttctccat
     8041 tccattcacc tgtgactcgg aagtgcattt atcatctcca taaaacaaaa cccgccgtag
     8101 cgagttcaga taaaagaaat cctcgtcagt gcgaggatgc tgttcattgc tgctatacac
     8161 ttttttgctc tcaacgtaag cggtagctca ttctgttggg ttggtgcagt tgcttttagg
     8221 aaatgctatt taccccttaa acgtcggctg aaagagctaa aatccatgca aaaaatttac
     8281 gcaattttgt gtattattgt gcagtaagta atgagctatt ttctgcgcaa aaaatggatg
     8341 gtaaatttgt ccggggcagg aaaaatttta tgggcgctaa acatgaaaaa agattcgtat
     8401 ccttatttga tttgcatgac agttttaggc ctgatcttta ttttcctttt cttctggtgg
     8461 cgggcagata tttacagggt cacgtttctt aatcagagta tatcccacta ttacattctg
     8521 tttagcatgg gaatagcttt tctgttatct ctgttttggg ttaagaaggg gatagtaaaa
     8581 caaagcggct ggaagagtct gtcagcatac cttaaggttt atgcagggat gtgcatattt
     8641 gctggatttt ttctgattat accccttaca acactaactt attttttgcc tggagagaca
     8701 tcgtcttatg ttgcaccgta tcggtatact tccggtagtt caaaaagttg ttctggagct
     8761 gaggtggatg accccgatct acatgagaat attcgcattt gctatccgta tggcaattat
     8821 gagtacgata atattatcta tgttgaaaag aaaattaata cattaggtgc ggtagtaaca
     8881 tatgcacaga ccgcgcgtga tgatactgaa tgagatagta tatagcgggc aagttttagt
     8941 taatttatcg aggtaatata atttacctcg actcgtttat tctggtatta atatttcgct
     9001 ttacgatcga tttttatctg atgatatcat gcggttttca tatactgact tactgtcttt
     9061 tctccgttag cgattttctc ctgctcagcg atgattttat ctttggcttc tagttaattt
     9121 cgctcacttc gaacctctct gtttactgat aagttccaga tcctcctggc aacttgcaca
     9181 agtccgacaa ccctgaacgg ccaggcgtct tcgctcatct atgggatcgc cacactcaca
     9241 acaatgagtg gcagatatag cctggtggtt caggcggcgc atttttattg ctgtgttgcg
     9301 ctgtaattct tcaatttctg atgctgaatc aatgatgtct gccatcttcc attaatccct
     9361 gaattgttgg ttaatacgct tgagggtgaa tgcgaataat aaaaaaggag cctgtagctc
     9421 cctgatgatt ttgcttttca tgttcaccgt tccttaaaga cgccgtttaa catgccgatt
     9481 gccagactta agtgagtcgg tgtgaatccc atcagcgtta ccgtttcgcg gtgcttcttc
     9541 agtacgctac ggcaaatgtc atcgacgttt ttatccggaa acggctgtct ggcttttttg
     9601 atttcagaat tagcctgacg ggcaatactg cgaagggcgt tttcttgctg aggtgtcatt
     9661 gaacaagtcc catgtcggca agcataagca cacagaatat gaagcccgct gccagaaaaa
     9721 tgcattcagt ggttgtcata ccaggtctct ctcatctgct tctgctttcg ccaccatcat
     9781 ttccagcttt tgcgaaaggg atgtggctaa cgtatgaaat tcttcgtctg tttctactgg
     9841 tattggcaca aacctgactc caatttgagc aaggctatgt gccatctcaa tactcgttct
     9901 taactcaaca ggagatgctt tgtgcatatc gcctcccgtt tattatttat ctcctcagcc
     9961 agccgctggg ctttcagcgg atttcggata acagaaaggc cgggaaatac ccagcctcgc
    10021 ttcgtaacgg agtagacgaa agtgatcgtg cctacgcgga tattatcgtg aggatgcttc
    10081 atcgccattg ctccccaaat acaaaaccaa tttcagccag tgcctcgtcc attttttcga
    10141 tgaactccgg caccatctcg tcaaaacccg ccatgtactt ttcatcccgc tcaaccacga
    10201 cataatgcag gccttcacgc ttcatacgcg ggtcatagtt ggcaaagtac caggcatctt
    10261 ttcgcgtcac ccacatgctg tactgcacct gggccatgta agccgacttt atggcctcga
    10321 aaccaccgag cctgaacttc atgaaatccc gggaggtaaa cgggcatttc agctcaaggc
    10381 cgttgccgtc actgcataaa ccatcgggag agcaggcggt gcgcatactt tcgtcgcgat
    10441 agatgatcgg ggattcagta acattcacgc cggaagtgaa ttcaaacagg gttctggcgt
    10501 cgttctcgta ctgttttccc caggccagcg ccttagcatt aacttccgga gccacaccgg
    10561 tgcaaacctc agccagcag
//
LOCUS       DAZGXS010000142        10565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGXS010000142 DAZGXS010000000
VERSION     DAZGXS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            159..1515
                     /gene="dcuC"
                     /locus_tag="ACHKG5_003976"
                     /pseudo
     CDS             159..1515
                     /gene="dcuC"
                     /locus_tag="ACHKG5_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1558..2055)
                     /gene="sspB"
                     /locus_tag="ACHKG5_003977"
     CDS             complement(1558..2055)
                     /gene="sspB"
                     /locus_tag="ACHKG5_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0869977.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2061..2699)
                     /gene="sspA"
                     /locus_tag="ACHKG5_003978"
     CDS             complement(2061..2699)
                     /gene="sspA"
                     /locus_tag="ACHKG5_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0869978.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3094..3486)
                     /gene="rpsI"
                     /locus_tag="ACHKG5_003979"
     CDS             complement(3094..3486)
                     /gene="rpsI"
                     /locus_tag="ACHKG5_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0869979.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="ACHKG5_003980"
     CDS             complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="ACHKG5_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0869980.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="ACHKG5_003981"
     CDS             complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="ACHKG5_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0869981.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5470..5868
                     /gene="zapG"
                     /locus_tag="ACHKG5_003982"
     CDS             5470..5868
                     /gene="zapG"
                     /locus_tag="ACHKG5_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0869982.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6022..7389
                     /gene="degQ"
                     /locus_tag="ACHKG5_003983"
     CDS             6022..7389
                     /gene="degQ"
                     /locus_tag="ACHKG5_003983"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0869983.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7479..8546
                     /gene="degS"
                     /locus_tag="ACHKG5_003984"
     CDS             7479..8546
                     /gene="degS"
                     /locus_tag="ACHKG5_003984"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0869984.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8610..9548)
                     /gene="mdh"
                     /locus_tag="ACHKG5_003985"
     CDS             complement(8610..9548)
                     /gene="mdh"
                     /locus_tag="ACHKG5_003985"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0869985.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9983..10453
                     /gene="argR"
                     /locus_tag="ACHKG5_003986"
     CDS             9983..10453
                     /gene="argR"
                     /locus_tag="ACHKG5_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0869986.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2605 a         2688 c         2628 g         2644 t
ORIGIN      
        1 aataatcata agcgcatatt ttttaatgaa aaatcacctc acctacaatt aaaaacacga
       61 catccgcacc ataaatagac ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt
      121 atgggattga cccttttctt ttgacacgga gttcaacaat gttcggcata attatatctg
      181 tcatcgtatt aattacgatg ggctatttga tcctgaaaaa ctacaaacct caggtggtgc
      241 tggctgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttaggt ggtgtactcg
      301 atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc ctgcgcatgc
      361 tgtccaaccg cattgccgga ttggggctgt cgattatggc ggtgggcggt tatgcccgct
      421 acatggagcg cacaggagcc agtcgcgcga tggtgagctt gttaagccgc ccgttaaaac
      481 tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggccaa atcatggcgc
      541 agtttattac cagcgcctcc ggcctgggta tgttgctgat ggtcacctta tttccgacac
      601 tggtgagtct gggagtaagc cgtctctctg cggtggcggt tatcgcaacc acaatgtcca
      661 ttgagtgggg gattctggaa acgaactcca tttttgcagc ccaggtagcg ggaatgaaaa
      721 ttgccactta cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg
      781 cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg
      841 aacaggcaga gcaaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg
      901 tgatgccgtt aatcctgatg ctcggctcgc tattccttgc ccacatcggg ctgatgcagt
      961 cagaactgca tctggtggtg gtgatgttac tgagtttgac gatgtttgtt gagttcttcc
     1021 gcaagcataa cttgcgcgaa acaatggacg atgtgcaggc gttttttgac ggcatgggta
     1081 cgcagtttgc caacgtggta acgctggtgg tcgcgggtga aatatttgcg aaaggcttaa
     1141 cgacgattgg cactgtcgat gcggttatca ggggtgcgga gcattctggt ctgggcggta
     1201 ttggcgtgat gattattatg gcgctggtca ttgccatttg tgccattgtg atgggctctg
     1261 gcaatgcgcc gtttatgtca tttgccagtc ttattccgaa tatcgcagcc ggactacatg
     1321 taccagcggt tgtaatgatt atgccgatgc attttgccac gacgctagcg cgcggtttcg
     1381 ccgattactg cggtggtggt cgttacgtca ggaattgcag gcgtttcgcc ttttgcggtg
     1441 gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat tgccacaatc
     1501 acgctatttt attaagtcat taaaagacaa aacaggccgc cagggcctgt ttttgtatta
     1561 cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc tgcggtggtt catcgtcagg
     1621 atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa cggtttcgtt
     1681 gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg cagcttcagg
     1741 ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg cagccagcgg
     1801 cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct catcattcgc
     1861 cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc cgtcacgcgc
     1921 atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt gcggcgtgag
     1981 ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac gtggtgttag
     2041 ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc tgcttcagtt
     2101 aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt cagctctttc
     2161 gcacccgggc cgctgaactc gatacccagt tgcggcagac gccacagcag cggagcaagg
     2221 tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc gaagaccggc
     2281 gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc agaagctgaa
     2341 ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg catgtacaaa
     2401 cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg gaaacgctca
     2461 tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc caccagggtc
     2521 ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc cttttccacg
     2581 tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg atggctatag
     2641 atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc gacagccatg
     2701 aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg ccagtcagaa
     2761 gttgtgttac ccagtaagga acgactctct ttgttcgaaa atcaaacaaa aaatgagcaa
     2821 tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga cctttgtggt
     2881 gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag ttgacttagt
     2941 cccttattgg cgatgtggtt tttgttttgc ctgtctgtca gacggcagca aaaagcaact
     3001 ttccattttt tacgctgatt cagaatttaa ccataaaaaa acccgccgaa gcgggttttt
     3061 tcgcaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact gcggacgacg
     3121 acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac gagtaacgaa
     3181 gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag cgcgggtgat
     3241 accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg tgatgtacag
     3301 gtccagtttc tcaaccatat cgaccagttc cagcggctga cgaactacca tgcgagcagt
     3361 ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac cgttgcccgg
     3421 tttgatgaaa acgcgagctg cggaactttt gcggcgacca gtgccgtagt attgattttc
     3481 agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg ccgcgtggtt
     3541 gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca gcgggccttt
     3601 tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag caatcatctc
     3661 ttcaaaggtc gcttgtttga taccaccgat gtggccggtg tggtgatagt acactttgtc
     3721 agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga tgtaatcacc
     3781 ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac gagccagttc
     3841 agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt cgcgttttac
     3901 ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat agttacacgt
     3961 tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc ggcaaaccta
     4021 ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact ttcttgtaac
     4081 gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt tgtgatttgt
     4141 ggcaggtttt aacccgctaa atgctcgcgc ttcagatact cttcgctttg catctcttgc
     4201 agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata aatttcatac
     4261 agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc atccaccagc
     4321 gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac atcaaacaac
     4381 atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg ggcgtcgacg
     4441 cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt cgctaatggc
     4501 cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag cgccaaccat
     4561 agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag atgcgcctga
     4621 gtgagtgtac gcagacgata atcaacacca gcgtccacgt tcattacatc acaatgctgt
     4681 ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg ataaagttcg
     4741 tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacag ggctttcatc
     4801 aggccgccaa gtagcatggc atcggtaata tcagagacaa aaaattcgtc aaaacagagc
     4861 acgtcagttt ctgctttaaa acgatcagca ataatttcca gcggatcggt ctgcccctgt
     4921 aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag gcgctgtttc
     4981 cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc gcgtcctaca
     5041 ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcccg tttaccccac
     5101 agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt gctattgatg
     5161 agttcctgat aaataatttc caagcggctg acggcctctt tttgaacgtc gtcgggttga
     5221 tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gagtaacgct ttgcatgatc
     5281 ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg
     5341 ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg gttatagtgg
     5401 cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa aagacaacgg
     5461 gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat cattattggt
     5521 gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa
     5581 ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag ccactttgcc
     5641 cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta tcagcacatg
     5701 gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat
     5761 cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc tcgcgactat
     5821 tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt tatttttcgg
     5881 gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat ctggtgtctc
     5941 attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt ttcttatcat
     6001 tcaggtacga gagcaggaat aatgaaaaaa caaacccagc tgttgagtgc attagcgtta
     6061 agtgtcgggt taactctctc ggcgtcattt caggccgttg cgtcgatccc aggccaggtt
     6121 gccgatcagg cccccctccc cagtctggct ccaatgctgg aaaaagtgct tccggcagtg
     6181 gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga agaattcaaa
     6241 aagttttttg gtgatgattt accggatcaa cctgcacaac ccttcgaagg tttaggctcc
     6301 ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt gattaatcag
     6361 gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa actgattggt
     6421 agcgatgacc agagcgatat cgccctgttg caaattcaaa acccgagcaa attaacgcaa
     6481 atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtggc ggtcggtaac
     6541 ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg acgcagcggg
     6601 ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa ccgcggtaac
     6661 tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac tgcaatcctt
     6721 gcgcctggcg gcgggagcgt cggaattgga tttgccatcc ccagtaatat ggcgcgaaca
     6781 ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg catcaaaggc
     6841 accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt
     6901 gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaaac gggcgatatt
     6961 attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc tcgtatcgcg
     7021 accaccgagc cgggcacgaa agtgaagctt ggcctgctgc gtaacggcaa accactggaa
     7081 gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat gatcacgcca
     7141 gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa aggtattaaa
     7201 atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa agacgatgtg
     7261 atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg
     7321 gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat ctatctgctg
     7381 atgcgttaat gtcgtaaacc gggcatcagg ctgacgtgtg atgtccggtt aactcgtggt
     7441 atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag ctcttacgtt
     7501 ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca
     7561 gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct gccagttata
     7621 atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt ggtttgaaca
     7681 ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc atggatcaac
     7741 gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct
     7801 tacaggatgg acgtgtattt gaagcattgc tggtgggatc tgactctcta accgatctgg
     7861 cggtacttaa aattaatgcc actggcggtt tacctaccat tccaattaat gcacgtcgcg
     7921 taccgcacat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc gggcagacca
     7981 ttacccaggg gattattagt gctacgggtc gaatcggtct gaacccgacc gggcggcaaa
     8041 acttcctaca aaccgatgct tccattaacc acggtaactc tggcggcgcg ctggtgaact
     8101 cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac gatggcgaaa
     8161 cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt atggataagc
     8221 tgatccgcga tggtcgcgtg atccgcggct acattggtat cggcggacgc gagatcgcac
     8281 cactgcacgc gcagggcggt ggtatagatc aactgcaagg gatcgtggtt aatgaagtgt
     8341 cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt atttcggtgg
     8401 ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcagaa attcgccctg
     8461 gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg caggtcacca
     8521 ttcaggaata tccggcaacc aattaaggcc agcgtgaaag acaaaaaaac cggagtctgt
     8581 gctccggttt tttattatcc gctaatcaat tacttattaa cgaactcttc gcccagggcg
     8641 atatctttct tgagcgtatc cagcatacct tccagcgcgt tctgttcaaa tgcgctcagg
     8701 gtaccgatag atttacgctc ttccacgccg tttttaccca gcagaagcgg ctgagagaag
     8761 aaacgggcgt actgaccgtc gccttcaacg taggcacatt cgacaacgcc ttgttcgccc
     8821 tgcagtgcgc gaaccagaga caggccaaaa cgtgcagcag cctggcccat agacagggtt
     8881 gccgacccgc caccggcttt cgcttcaacc acctcagtac ccgcgttctg gatacgtttg
     8941 gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga caacagcggc
     9001 agaatggtaa caccagagtg accgccaata accggcactt caacttcgcc tggctgtttg
     9061 cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat ccagcgtggt aacgccgaac
     9121 agtttgtttt tgtcataaac accggctttt ttcagcactt cagcagcaat cgcaactgtg
     9181 gtgttaaccg ggttagtgat aataccaatg cacgctttcg ggcaggtttt cgcaacttgc
     9241 tgtaccaggt ttttcacgat gccggcgtta acattaaaca ggtcggaacg atccataccc
     9301 ggtttacgcg ctacacctgc agagataaga acgacatctg cgccttccag cgccggagtc
     9361 gcatcttcac cagaaaaacc tttgattttc acagcagtag ggatatggct cagatcgacg
     9421 gccacaccgg gagtcactgg agcgatatca tacagagaga gttctgaacc tgaaggcagt
     9481 tgggttttta acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg
     9541 actttcatcc taaactcctt attatattga taaactaaga tatgttgctc cgctgccgcg
     9601 accttaatcc acaaaattgc cgtttacaat gaccacatct caagaatgtg tagtcacgca
     9661 agtttagcgt ttatgcattt aattgccgta atcaggaacc taagttacgt aattaacaat
     9721 cgcttatgca ttagcgcaac attccaacag gtggtgacaa tataccctac cgttcagcca
     9781 aaacaacatc aatttgataa caattaattt acttttaagc agaatttgca tgccgtgacg
     9841 caggcatatt tctcaataac gaaatttgat aaaatcccgc tctttcataa cattatttca
     9901 gccttcttca gggctgactg tttgcataaa aattcatctg tatgcacaat aatgttgtat
     9961 caaccaccat atcgggtgac ttatgcgaag ctcggctaag caagaagaac tagttaaagc
    10021 atttaaagca ttacttaaag aagagaaatt tagctcccag ggcgaaatcg tcgccgcgtt
    10081 gcaggagcaa ggctttgaca atattaatca gtctaaagtc tcgcggatgt tgaccaagtt
    10141 tggtgctgta cgtacacgca atgccaaaat ggaaatggtt tactgcctgc cagctgaact
    10201 gggtgtacca accacctcca gtccattgaa aaatctggtg ctggatatcg actacaacga
    10261 tgcagttgtc gtgattcata ccagccctgg cgcggcgcag ttaattgctc gcctgctgga
    10321 ctcactgggc aaagcagaag gtattctggg caccatcgct ggcgatgaca ccatctttac
    10381 cacccctgct aacggtttca ccgtcaaaga cctgtatgaa gcgattttag agctgttcga
    10441 ccaggagctt taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt gcttatccct
    10501 ctcaaccccc tgctttcccc tgcgattaat ttaacgaata gtgcgtttta ctgcgacatg
    10561 tcatt
//
LOCUS       DAZGXS010000143        10374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGXS010000143 DAZGXS010000000
VERSION     DAZGXS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            133..708
                     /gene="ssuE"
                     /locus_tag="ACHKG5_003987"
     CDS             133..708
                     /gene="ssuE"
                     /locus_tag="ACHKG5_003987"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0869987.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            701..1660
                     /gene="ssuA"
                     /locus_tag="ACHKG5_003988"
     CDS             701..1660
                     /gene="ssuA"
                     /locus_tag="ACHKG5_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0869988.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1657..2802
                     /gene="ssuD"
                     /locus_tag="ACHKG5_003989"
     CDS             1657..2802
                     /gene="ssuD"
                     /locus_tag="ACHKG5_003989"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0869989.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2814..3605
                     /gene="ssuC"
                     /locus_tag="ACHKG5_003990"
     CDS             2814..3605
                     /gene="ssuC"
                     /locus_tag="ACHKG5_003990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0869990.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3602..4369
                     /gene="ssuB"
                     /locus_tag="ACHKG5_003991"
     CDS             3602..4369
                     /gene="ssuB"
                     /locus_tag="ACHKG5_003991"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0869991.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4412..7024)
                     /gene="pepN"
                     /locus_tag="ACHKG5_003992"
     CDS             complement(4412..7024)
                     /gene="pepN"
                     /locus_tag="ACHKG5_003992"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0869992.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7290..8492
                     /gene="pncB"
                     /locus_tag="ACHKG5_003993"
     CDS             7290..8492
                     /gene="pncB"
                     /locus_tag="ACHKG5_003993"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0869993.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8661..10061
                     /gene="asnS"
                     /locus_tag="ACHKG5_003994"
     CDS             8661..10061
                     /gene="asnS"
                     /locus_tag="ACHKG5_003994"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0869994.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2336 a         2647 c         2798 g         2593 t
ORIGIN      
        1 accctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg gcattttctt
       61 gtctctccga cagactgagc gaattgttat caatgttaac aaaacaagaa caattggtta
      121 taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc
      181 agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgttga ggtttatcac
      241 tggaatctgc aaaacttcgc cgcggaagat ctgctttatg ctcgtttcga tagtccggca
      301 ctccatacct tcaccgaaca gttgcaacag gctgacgggc tgattgtcgc cacgcctgtg
      361 tataaagccg cttattccgg tgcgctgaaa accctgctcg acctgctgcc agaacgtgcg
      421 ttgcaaggca aagtggtgct accgctggcg acgggcggta cggtggccca tctgctggcg
      481 gtcgattatg cccttaagcc agttttaagt gcgctgaaag cccaggagat cctgcacggc
      541 gtgtttgccg atgactcaca agtaattgat taccatcaca aaccccagtt cacgccgaat
      601 ttacaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat
      661 gttcaggttc ctgatcttca ttctctgcga ggtaatgccc atgcgtaaca tcattaaact
      721 ggcgctggtg ggattgctta gcgtctctac gattgtggtt gctgcagaat cctcgcctga
      781 agcgttacgt ataggctatc agaaaggcag tattggtatg gtattggcaa aaagccacca
      841 gttactggaa aaacgctatc cgcaatcaaa aatctcgtgg gtggagttcc ccgctggccc
      901 gcaaatgctt gaggcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc
      961 gccaatcttc gcccaggctg ccggggctga tttgctgtac gtgggcgtcg agccgccgaa
     1021 gccaaaagcc gaggtgattc tggtggcaga aaacagcccg atcaaaaccg cagccgatct
     1081 taaaggccac aaagttgcgt tccagaaagg ttccagttca cacaatcttt tactgcgcgc
     1141 actacgccag gccggactta agtttactga catccagccc acttatctga cgccagctga
     1201 tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta
     1261 ctccgctgca ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg atctcaatca
     1321 aaccggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg cttttattca
     1381 gggtgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag
     1441 tatcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cttacttaga
     1501 tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct tacagcagca
     1561 aacggcagat ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata ttcgccaacg
     1621 catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat gttctggttt
     1681 ttaccgaccc atggtgacgg gcattatctg ggaacggaag aaggttcacg cccggttgat
     1741 cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc ttggctatac cggtgtgctg
     1801 atcccaacgg ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat gatcccggtg
     1861 acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc taccgttgcc
     1921 gcccgccagg ccgccacgct tgaccgtctc tcaaatggac gtgcgttgtt taacctggtc
     1981 acaggcagcg atccacaaga actggcaggc gacggcgtgt tccttgatca tagtgagcgc
     2041 tacgaagcct cggcggaatt tacccaggtc tggcggcgtt tattgctggg ggaaaccgtc
     2101 gatttcaacg gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc gccgattcaa
     2161 cagccgtatc ctccgcttta ctttggcgga tcgtcagacg tcgcccagga gctggcggca
     2221 gaacaggttg atctctacct cacctggggc gaaccgccgg aactggttaa agagaaaatc
     2281 gaacaagtgc gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg cattcgtctg
     2341 catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg gttaatctcg
     2401 catcttgatg atgagactat cgccaaagca caagccgcat tcgcccgcac ggattccgtg
     2461 gggcaacagc gaatggcagc gctacataac ggcaagcgcg acaatctgga gatcagcccc
     2521 aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct ggtgggcgat
     2581 ggtcctacgg tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga cagctttgtg
     2641 ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct gttcccacat
     2701 ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca aggcgaagcg
     2761 gtggagaatg attttatccc tcgcagagtc gcgcaaagct aaggaagaag ataatggcaa
     2821 cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtga
     2881 cggtgtggca actggcctcc tcggttggct ggttgtcgac gcgtattttg ccctcaccgg
     2941 aaggggtggt gatggcgttc tggacgctct cagccagcgg cgaattgtgg cagcatctgg
     3001 cgatcagctc ctggcgggcg ctgattggtt tttcaattgg cggctcgctg gggttgattt
     3061 tgggactgat tagcggactg tcgcgctggg gagaaaggct gctggatacc tcaattcaga
     3121 tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg tttggcattg
     3181 atgaatccgc aaaaatcttt ctggtggcgc tcggtacgct attccccatt tatatcaaca
     3241 cctggcatgg catccgtaat atcgatcgcg ggttggtgga gatggcgcgt agctatggat
     3301 tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc tcaattatgg
     3361 tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc gaaaccattt
     3421 ctgccaattc aggcattggt tatctggcga tgaatgcgcg ggagtttttg caaacggacg
     3481 tggtggtagt cgctattatt ctttacgccc tgctcggcaa acttgctgac gtcagcgcgc
     3541 agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag gaggccacgg
     3601 tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag taagcaaaca
     3661 ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag gtcagtttgt
     3721 ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct
     3781 ggaaacgcca accgctggcg atgtgttagc gggcaccaca ccgctggctg aaattcagga
     3841 agacacgcga atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg tgattgataa
     3901 cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt tggctgcggt
     3961 aggactggag aatcgcgccg gggaatggcc tgccgcactt tctggcgggc agaaacagcg
     4021 agtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg atgaaccgct
     4081 cggtgcgctg gatgccttaa cgcgactcga gatgcaggat ttgattgtgt cactttggca
     4141 ggagcacggt tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc
     4201 tgatcgggtg ttgttaattg aagagggaaa aattggtctg gatttgacgg tggatattcc
     4261 ccgtccgcgc cggttagggt cggtgcgatt agcggagctg gaagcggaag tgttgcaacg
     4321 ggtgatgcag cgtggtgaat ctgaaactcg cctgagaaaa caggggtaaa atccccgaat
     4381 ggcggcgcta ttgccgccat tcggggattt attaagccag tgctttggtg atcttctcgt
     4441 acagatcgcc agatagattt tccagccctt tcaactgttc cagcgccgcg cgcattttct
     4501 cctgacgttt ggcatcataa cgtttcaggc gaatcagcgg ttcaatcagg cgtgaagcta
     4561 cctgcgggtt acggctgttc aggtcggtaa gcatttccac caggaactgg taaccgctcc
     4621 catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc aacgaacgaa
     4681 tgcggttcgg gttgctcatg gtaaatgagc gatgctgcaa caggccgcgc accgtctcca
     4741 gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc accagaccat
     4801 cctgatgcca cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc agctgtgcag
     4861 caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca tggtattgct
     4921 tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc aggcaggcat
     4981 tacgcagagt gcgttttgca atatcttcat gctcaacacg gtactcgctc tggtaattcg
     5041 cgttgtaaat agccagtagc tcatccgcca gttcagtcgc cagagtacga gtgagtgctt
     5101 cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc atttcattga
     5161 cagaaggcag cgtcaggatt tctgccgcca gcgccggatc aatcttctca tcgagcagca
     5221 ccgcgcggaa agcgtcagcc acatgtaccg gcagagacag cggctgccct tgctgatgac
     5281 gggcgacgtt cagcttgatg taggttgcca gcaaactttg cgccgcatcc caacgggaaa
     5341 aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac ttatattcca
     5401 gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag tagacattat
     5461 caaaaacaaa ggtctgttcc gcctgggtga cgttcagcac ggaattcacc ggatgaccgc
     5521 ctttctgcaa cgggatcact ttgccttcgt tgtcatacaa ttcgatggca aacggaatat
     5581 gcagcggctg tttttctgct tgatccggcg tggctggcgt gcgctggctg atggtcaggg
     5641 tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt cacaaccggc gtaccggact
     5701 ggctgtacca acggcggaaa tgggagagat cgacattcga cgcatcttcc atcgcctgaa
     5761 cgaaatcgtc gcaggtcgct gcactgccat catggcgctc gaaatagagc tgcatccctt
     5821 tctggaagtt ttcttcgcca agcagagtgt ggatcatacg aatcacttcc gcgcccttct
     5881 cgtaaacggt cagggtgtag aaattgttca tctcaatgac catgtccggg cggattgggt
     5941 gcgccatcgg gctggcgtct tccgcaaact gcaatccgcg catggtgcgc acattattga
     6001 tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg aagacggtta
     6061 aaccttcttt caggctgagc tggaaccagt cgcggcaggt cactcggtta ccggtccagt
     6121 tatggaaata ttcatggccg ataacgcgtt caatatcgag gtaatctttg tcggtggcgg
     6181 tatcggtgcg ggccagcaca tatttggagt taaagatatt cagaccctta ttctccattg
     6241 cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca tactccaggc
     6301 caaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc cacggcgcgc
     6361 gatcaaggtt gccgcgatcg acgtacagct ccagtgctac ttcgcgacca gaacgcgtgg
     6421 taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg tagcacggtt
     6481 tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt ttccagttcg ccttgcgcaa
     6541 cgcggttacc gttggaaagc aggaagggat atttggtttt atcggcaata attttggtgg
     6601 taaaacgcgc cagcacgtcc gggcggtcga gataatacgt aatatggcgg aaaccttcgg
     6661 cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc gccgtattcg
     6721 ccgccgggct tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa ttactgatga
     6781 ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc atcattaata tgaacagaaa
     6841 ccagtttgag gtcttcgccg ttgagacaaa gcggagcatc tgatgcacca tgacggacag
     6901 cctggctgac cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc aagtcaatat
     6961 cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt ggctgttgag
     7021 tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc ctgttgcgta
     7081 aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg ctacattttt
     7141 ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg atcggggttc
     7201 aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt gtactaaacg
     7261 ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct gttctgcact
     7321 cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt catcattatt
     7381 acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg ggtatttatg
     7441 ccgatgctat tcgtgaacag gttcaggcga tgcagcacct gcgcctgcag gatgatgaat
     7501 atcagtggct ttctgccctg cctttcttta aggccgacta tcttaactgg ttacgcgagt
     7561 tccgctttaa cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg gatattcgtt
     7621 taagcggccc gtggcgtgaa gtcatcctct gggaggttcc tttgctggcg gttatcagtg
     7681 aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc gacacgctgg
     7741 aaagcaaatt agtcgacttc tcggcgttaa ccgctggtct tgatatgtcg cgcttccatc
     7801 tgatggattt tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc atcgttaagc
     7861 gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg cgtcggcttt
     7921 ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcacatcag caaatcagcc
     7981 cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagag tatcccgacc
     8041 aacttggcat tgcattaacc gactgcatca ctatggatgc tttcctgcgt gatttcggtg
     8101 tcgagttcgc tagtcggtat cagggcctgc gtcatgactc tggcgacccg gttgaatggg
     8161 gtgaaaaagc cattgcacat tatgaaaagc tggaaattga tccacagagt aaaacgctgg
     8221 ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac ttctcttccc
     8281 gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc caggtaaaac
     8341 ccctgaatat tgtgattaag ttggtagagt gtaacggtaa accggtggcg aaactttctg
     8401 acagccctgg caaaactatc tgccatgata aagcgtttgt tcgggcgctg cgcaaagcgt
     8461 tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat cggctccctt
     8521 tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg tctgattgca
     8581 gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta ttttttccga
     8641 ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc cagggccgcg
     8701 tagccgttga cagcgaagtc accgtacgcg gatgggtacg tacccgccga gattcaaaag
     8761 ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta caggctgtca
     8821 tcaataattc tctgcccaat tacaatgaag acgtcctgcg tctgaccacc ggctgctcgg
     8881 tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt gaaattcaga
     8941 ccagcaaggt tgaagttgct ggttgggttg aagatccaga cacttacccg atggcggcaa
     9001 aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga
     9061 ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg
     9121 agcagggatt cttctgggtt tcaacgccac tgattactgc gtctgatacc gaaggggctg
     9181 gcgaaatgtt ccgcgtttct acgctggatc tggaaaatct gccgcgtaac gatcagggca
     9241 aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta tctggccagt
     9301 tgaacggcga aacctacgct tgcgcattat ccaaaattta taccttcggc ccgactttcc
     9361 gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg gagccggaag
     9421 tggcgtttgc taacctgaac gatatcgcgg gtctggctga agccatgctg aaatatgtct
     9481 tcaaagcggt tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa cgcgtagata
     9541 aagatgccgt ttcacgtctg gaacgcttta ttgaggccga tttcgcgcag gtggattaca
     9601 ccgaagcagt aaccattctc gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg
     9661 gcgtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt aaagcaccgg
     9721 tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctc aacgaagacg
     9781 gtaaaaccgt tgcggctatg gatgttctgg ctccgggcat cggtgagatc attggtggct
     9841 cccagcgtga agagcgtctg gacgtgctgg acgagcgtat gctggaaatg ggcctgaaca
     9901 aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg cattccggtt
     9961 tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg tgtgcaaaac gtgcgtgatg
    10021 tgattccgtt cccacgaact ccgcgtaacg ccagcttcta atttagcgtc tacaagagcc
    10081 agcacccgct ggctcttttt tatcctttct gtcaagttat ctgtttgtta agtcaatcaa
    10141 tctatttgca accccgccat aacgatcatc ctgttacgga atattacatt gcaacattta
    10201 cgcacaaaaa ataacccgca ttcttattgc ggattagttt tttcttagct gatagcacaa
    10261 ttttcatact atttttttgg cattctggat gtctgaaaga ggattttggg ccaggtcgat
    10321 aaagtttcca tcagaaacaa aatttccgtt tagttaattt aaatataagg aaat
//
LOCUS       DAZGXS010000144        10329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGXS010000144 DAZGXS010000000
VERSION     DAZGXS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            158..1840
                     /gene="ydeN"
                     /locus_tag="ACHKG5_003995"
     CDS             158..1840
                     /gene="ydeN"
                     /locus_tag="ACHKG5_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0869995.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1892..3049
                     /gene="ydeM"
                     /locus_tag="ACHKG5_003996"
     CDS             1892..3049
                     /gene="ydeM"
                     /locus_tag="ACHKG5_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0869996.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3015..3137)
                     /locus_tag="ACHKG5_003997"
     CDS             complement(3015..3137)
                     /locus_tag="ACHKG5_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0869997.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3340..5025
                     /gene="yddA"
                     /locus_tag="ACHKG5_003998"
     CDS             3340..5025
                     /gene="yddA"
                     /locus_tag="ACHKG5_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0869998.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5063..7435
                     /gene="yddB"
                     /locus_tag="ACHKG5_003999"
     CDS             5063..7435
                     /gene="yddB"
                     /locus_tag="ACHKG5_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP0869999.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7492..10275
                     /gene="pqqL"
                     /locus_tag="ACHKG5_004000"
     CDS             7492..10275
                     /gene="pqqL"
                     /locus_tag="ACHKG5_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0870000.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3024 a         2291 c         2266 g         2748 t
ORIGIN      
        1 tagtagaaca aaaagtaatg tcaacgcttt atggactgtt atttcccttt ttttagctaa
       61 atctgctatc tctttatgtg actaacttca cttacatcca cttatttctc ttcgtaaaat
      121 tactttggaa ttaagtacaa taagaagagg aacatttatg aagtctgcat taaagaaaag
      181 tgtcgtaagt acctcgatat ctttgatact ggcatctggt atggctgcat ttgctgctca
      241 tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac gttgctttct cagactttac
      301 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactgacca tggatgatct
      361 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      421 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cgcaaaaatc
      481 aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg gctatgtggc
      541 gcacggtgtt tccggcccct cccgcgccgc aataatgacc ggtcgagcgc ccgcccgctt
      601 tggtgtctat tccaatactg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      661 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      721 aatcagtaat gtgccggtac cggaagataa acaaacacgt gactatcatg acaacttcac
      781 cacattttct gcggaagaat ggcaaccgca aaaccgtggc tttgattact ttatgggatt
      841 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaagaatc gtgaacgtgt
      901 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      961 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctgcc
     1021 aaatgataat cctgcaccgg agcaatatca gaagcaattt aataccggta gtcaaacagc
     1081 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
     1141 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
     1201 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
     1261 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1321 ttatgacaag ctgatttccg caatggattt ctacccgaca gctcttgatg cagccgatat
     1381 cagcattcca aaagacctta agctggatgg cgtttccttg ctgccctggt tgcaagataa
     1441 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1501 tgaggaaaat attccattct gggataacta ccacaaattt gtccgtcatc agtcagacga
     1561 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1621 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1681 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1741 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1801 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1861 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1921 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1981 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgcagcgtc
     2041 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     2101 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     2161 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     2221 gtttctggtt ggtatctcga tcgatggacc ccaggagcta catgaccgtt acagacgcag
     2281 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2341 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga
     2401 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctcga
     2461 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2521 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2581 taaaaacgat gtgggtgaaa ttttcatccg tcagtttgaa agctttgtca gccgtttttt
     2641 ggggaatggg cataccagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2701 aagtaatgga gatatttacg aatgcgatca ttttgtctat ccacagtaca aacttggaaa
     2761 cattaataaa tctgaactta aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2821 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgccctaa
     2881 gcatcgtatt actaaagtaa acgatgagac tgtttcctat ttttgcgaag gttataaaat
     2941 ccttttttca accatggtac cttatatgaa cgccatggtg gagttagcca agaacagagt
     3001 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     3061 tttagctcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     3121 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ttggcttacg tggtatgcgt
     3181 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     3241 atttctatat atttcatatt gttaaatgat acttaaataa atttcatcag taaatcattg
     3301 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     3361 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     3421 aataaaactt cagtgttgtt aatcattatt atcctcgcca tgatccttgg cgtggttaaa
     3481 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     3541 accgataagc tttggcagct tgtcctttgg ttccctgccc tgctggggat ctttgtatta
     3601 atatccgtaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3661 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3721 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3781 atcagcaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3841 tttaccgtta ttctttggca aagcgcgggt atgctctcat tcactgttgg tggaacagaa
     3901 tggaatatcc aggggtatat ggtctatacc gttgtgctca ttgtgatcgg tggaacttta
     3961 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     4021 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     4081 aatgctgagt ctctacaacg ccaggagttg agcgacaatt ttcataccat taaagagaac
     4141 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcagaatat ttattcgcgc
     4201 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     4261 ctgggcggac tgatgaaatc gcgccaggca tttatgctgg tatcgaacaa tctaagctgg
     4321 tttatttata aatatgatga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     4381 ttccatcaac tcaccgaaca gcgccctacg aataagccta aaaattgcca acatgcggta
     4441 caagtggctg acgcgagtat tcgtactcct gataataaga tcatattaga gaacctgaac
     4501 tttcatgttt cgccaggcaa atggctatta ctgaaaggtt actctggtgc gggaaaaacc
     4561 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     4621 gccgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa ccggcttact gaaagagatt
     4681 atttgtaaag cacttcccct gcccgtagac gataaatcat tgagcgaagt actgcatcag
     4741 gttggtcttg ggaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4801 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4861 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatccg cttactgcgt
     4921 ctagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4981 tggaacctgg ccgatgatat ttgtgacatt agcgcggttt tataaaaaca ataaaagccc
     5041 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     5101 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgcattttt ttgaagagat
     5161 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     5221 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     5281 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gcccggagaa
     5341 aatctctatt cacggtgcgt cgccctatca gaatgcctat ttgattgacg gtattagtgc
     5401 cactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     5461 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     5521 ttttgtgccg gttgaattcg gtcgcttcaa tggcggggta attgatgcaa agttcaaacg
     5581 cttcaacgct gatgatagca gcgtgaaact gggttatcgc actacgcgtt cggactggtt
     5641 aacatcgcat atcaatgaga ataacaagag cgcatttaat caaggctctt caggaagtac
     5701 ttattactcc ccagatttta aaaagaactt ttataccttg tcgtttaatc aggaactcgc
     5761 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5821 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5881 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5941 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     6001 gggtaataaa tcctatggta tggcatggga tatggatacg cagctcgcat gggccaaact
     6061 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     6121 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgcaccc gtggcggatt
     6181 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     6241 attcgccgtg ggtaatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     6301 tgcgtggact gaacgccata accagtctga atcctatgtg attaatgctg ccggaaagaa
     6361 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacact
     6421 gtatatggcg gatcgcatta gctggcgtaa tgtgtcatta atgcccggcg tgcggtatga
     6481 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     6541 ttttgctgat caaacctcaa tgattacagc aggttataac cgttactatg gcgggaatat
     6601 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6661 aaccctgacg cgttatcagg atttgaaaac gccttataac gatgaactgg caatgggatt
     6721 gcagcaaaaa atcggtaaga acgttattgc gcgcgcaaac tatgtttacc gtgaagcgca
     6781 tgatcaaatc agcaaaagca gtcgtaccga cagcgcgact aaaaccacca ttactgaata
     6841 taacaacgac ggcaaaacca aaacgcattc gttcaacctc agttttgaac tggccgaacc
     6901 cctgcatatc agccaggtag atattaaccc gcaaattgtc tttagctata tcaagagcaa
     6961 gggcaacttg tcgttaaaca atggttatga ggagagcaat accggtgata accaggtggt
     7021 ttataacggt aatcttgtct cttacgatag cgttccagtg gcagatttta ataacccatt
     7081 aaagatctcc ttaaacatgg atttcacgca tcaaccgagc gggttggtgt gggcgaatac
     7141 gctggcctgg caagaagcgc gtaaagctcg cattatcctg ggtaagacaa atgcgcaata
     7201 catcagcgaa tattcagatt acaagcagta tgttgacgaa aaactggata gcagcctgac
     7261 ctgggacacc cgcttgtcct ggacgccaca atttctgaaa caacaaaacc tgacgatcag
     7321 tgccgatatt ctcaatgtac tggatagcaa aaccgcggtt gatacaacga ataccggtgt
     7381 ggcgacctac gccagtggcc gtactttctg gcttgatgtc agcatgaaat tttaatagtt
     7441 cggattttac ttaaccgggt aaattcgccc ggttttcgca tggagataat tatgagaaac
     7501 ctctgtttct tactgacgtt agtggcaact ctgttgctcc ccgggcggct gattgccgcc
     7561 gccttaccgc aggatgaaaa gttaattacc gggcaactgg acaatggctt gcgatatatg
     7621 atttatccgc atgctcatcc aaaggatcag gtaaatttat ggctgcaaat tcataccggt
     7681 tcattgcagg aagaagacaa tgagcgcggc gtggctcatt ttgtagaaca tatgatgttt
     7741 aacggcacaa aaacatggcc gggtaataaa gtcatcgaaa catttgagtc aatgggcctg
     7801 cgttttggtc gcgatgttaa tgcctatacc agctatgacg aaacggtgta tcaggtgagt
     7861 ttgccaacta cgcagaaaca aaatctgcaa caagtgatgg caatcttcag tgaatggagt
     7921 aatgccgcaa cctttgaaaa actcgaagta gacgctgaac gcggcgtaat tactgaggaa
     7981 tggcgtgccc atcaggatgc gaaatggcgc acctctcagg cgcgccgccc tttcctgctg
     8041 gcaaataccc gtaatttaga ccgtgaacct atcggcctga tggatactgt cgccacggtc
     8101 acaccggcac aattgctcca attttatcaa cgctggtatc aaccaaataa tatgaccttt
     8161 atcgtggttg gcgatatcga cagtaaagaa gcgctggcgc tgataaagga taatttaagt
     8221 aagcttccgg ctaacaaagc agctgaaaat cgcgtctggc cgacaaaagc cgaaaaccac
     8281 ctgcgcttta atatcattaa tgataaagaa aaccgggtga acggcatcgc actctattat
     8341 cgcctgccaa tggtacaagt gaacgatgag caaagcttta tcgaacaagc tgaatggagc
     8401 atgttagttc agctgttcaa tcaacgtctg caggaacgta tacagtcggg cgagttgaag
     8461 actatttctg gcggcactgc gcgcagcgtt aaaattgcac ccgattatca gtcgctgttt
     8521 ttccgtgtaa atgcacgaga cgataatatg caggatgctg cgaatgcatt aatggaagag
     8581 ttggcaacca ttgatcagca tggtttttct gctgaagaac tcgatgatgt caaatctacc
     8641 cgcctcacct ggctgaaaaa tgcggttgat cagcaagctg aacgtgattt acgtatgctg
     8701 accagtcgcc tggcatccag ctcattaaat aatacgccgt tcttgtcgcc ggaagagaca
     8761 tatcaacttt cgaaacgtct gtggcagcaa attaccgtgc aaagtctggc ggaaaaatgg
     8821 cagcagttaa gaaagaacca ggacgcattt tgggagcaaa tggtaaacaa tgaggttgcc
     8881 gccaaaaaag cattgtctcc tgcggctatc ctggcgctgg aaaaggagta cgccaacaaa
     8941 aagctggcgg cttacatctt cccaggcaga aatttatcgt taacagtaga cgctgaccca
     9001 caggcggaaa ttagcagcaa agaaacgctg gctgagaatc tgacatcatt aacactttcc
     9061 aatggtgcaa gggttattct ggcaaaatcc gcgggtgaag agcaaaagct acaaattatt
     9121 gccgtatcga ataaaggcga tttaagtttc cctgcgcagc aaaaatcact tatcgcgctg
     9181 gcaaataaag cagttagcgg aagcggcgtt ggcgaactct cctcttccag cctgaaacgc
     9241 tggagtgcgg aaaactcggt aaccatgagc agtaaagtca gtggcatgaa tacgttgctc
     9301 tccgttagcg cgcggactaa taaccctgaa cctggttttc agttgattaa ccagcgaatc
     9361 acccacagca cgattaacga caatatttgg gcatcgctac aaaatgctca aattcaggca
     9421 ttgaaaacgc tcgaccagcg tccagcggag aaattcgccc agcagatgta tgagacgcgc
     9481 tatgctgatg accgcacgaa attactgcaa gaaaatcaga ttgcacagtt tactgccgca
     9541 gatgcgctgg ctgccgatcg ccagttgttt tcatctccag cggatatcac gtttgtcatt
     9601 gtcggtaatg tcgcagaaga caaactcgtg gcgttaatta cgcgttactt aggatcaatc
     9661 aaacactctg attcgccatt agccgcaggt aaaccattaa ctcgcgcgac ggacaacgca
     9721 tcggttactg taaaggaaca aaatgaacct gtggcacagg tttcacagtg gaagcgttat
     9781 gattcccgga cacctgttaa tctggcgacg cgtatggcgc tcgatgcttt taacgtcgca
     9841 ctggcaaaag atctacgcgt taatattcgt gaacaggcat ctggggcata cagcgtttct
     9901 tctcgcctct cggttgatcc tcaggctaaa gatatcagtc atttgctggc ttttacttgt
     9961 caaccagaac gacatgatga actgttaacg ttagcgaatg aagtgatggt taagcgtttg
    10021 actaaaggga tcagtgagca agaactgaac gaataccagc aaaacgttca gcgcagcctc
    10081 gatatccaac agcgtagcgt tcaacaatta gcgaacacta ttgtaaatag tcttattcaa
    10141 tatgacgatc ctgctgcatg gactgagcag gagcaattgt tgaaacaaat gacggtagag
    10201 aatgttaaca ctgccgttaa acaatatctt tctcatccgg taaatactta taccggagta
    10261 ttattgccaa aataataaca gccccgtcaa cacatcgttg gcggggattt tagcaatatt
    10321 cgctatttt
//
LOCUS       DAZGXS010000145        10218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGXS010000145 DAZGXS010000000
VERSION     DAZGXS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            43..936
                     /locus_tag="ACHKG5_004001"
     CDS             43..936
                     /locus_tag="ACHKG5_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870001.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            943..1857
                     /locus_tag="ACHKG5_004002"
     CDS             943..1857
                     /locus_tag="ACHKG5_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0870002.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLCFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2551..2769
                     /gene="yiiE"
                     /locus_tag="ACHKG5_004003"
     CDS             2551..2769
                     /gene="yiiE"
                     /locus_tag="ACHKG5_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0870003.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2986..3228
                     /locus_tag="ACHKG5_004004"
     CDS             2986..3228
                     /locus_tag="ACHKG5_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0870004.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3558..4487)
                     /gene="fdhE"
                     /locus_tag="ACHKG5_004005"
     CDS             complement(3558..4487)
                     /gene="fdhE"
                     /locus_tag="ACHKG5_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0870005.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4484..5119)
                     /gene="fdoI"
                     /locus_tag="ACHKG5_004006"
     CDS             complement(4484..5119)
                     /gene="fdoI"
                     /locus_tag="ACHKG5_004006"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0870006.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5116..6018)
                     /gene="fdoH"
                     /locus_tag="ACHKG5_004007"
     CDS             complement(5116..6018)
                     /gene="fdoH"
                     /locus_tag="ACHKG5_004007"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0870007.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6031..9081)
                     /gene="fdnG"
                     /locus_tag="ACHKG5_004008"
     CDS             complement(6031..9081)
                     /gene="fdnG"
                     /locus_tag="ACHKG5_004008"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8494..8496),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0870008.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQPEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9275..10108
                     /gene="fdhD"
                     /locus_tag="ACHKG5_004009"
     CDS             9275..10108
                     /gene="fdhD"
                     /locus_tag="ACHKG5_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0870009.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2589 a         2439 c         2597 g         2593 t
ORIGIN      
        1 tatgtagcat gtttgctctt aaaccgtggg aaggaggctg taatggttgc caatattaat
       61 ctcattaaac aagaaagtta ttctgttgtt aatttagaaa aacaactaat gagtaatgaa
      121 tcaccgaagc aatcgctatt aaatgatttt tcacatcgcc ttaaaatgga aggtgaggaa
      181 caactgttcc attgtgttaa cgaactgaag tatggtttgt cgagtaataa tgaatatgac
      241 ctgtttaaaa atagagaaac ctcactgtgg aaacgcaaag taccagaaag cgcagcgctg
      301 gcaatgacgg gaacagtata taaattattt ggtagtcgct tactcaacgc ccgcaatcag
      361 ttagataaaa atctcctcga aatggctatg caaatggttt atggcaaaga tatggtgaca
      421 aagtccgggg atatattgat tgatatacaa ttaaataagg atggcagcct ccagagtaag
      481 cactacacat ttgatgtagg tgaagttata aacagttata atattgacct ctttgtgaat
      541 gcttcgagta ttaatcaaac ttacttacat gataaaaatc ctggtgatgt tattagcctg
      601 tatggcactt tagataacat tcctcttgta gttaaggaat gcacgggtaa aaatcatcaa
      661 attaacgtta aagccaaatt gccacaagaa cgaagtgaaa aagttgagga tatgtgtgaa
      721 agaatgagag gtggcatctc tatgtttaat cacaccacaa aaacagctgg taatatagag
      781 cataatttgc agctaagttt tctcgtggat tcacggccgt cgatcaccaa taaatactca
      841 gccaaagatg tagcgccaga catacttgta ttgcccgtca acgtatatca tgcggatttt
      901 aaaattaaaa tcaataatgc agtagagaag acttagtcaa ttgtgttatg tgctgtgcaa
      961 aaaaatctcc atgcagcaag gaaggttctt agcgggataa taggtaatgt accaaaaaat
     1021 aataaaccaa aaacaatcat ttttgattta tgttttctta gtgcaattaa gcagaaagtt
     1081 tttttgggca gtgaacaacc gttgtttgta aagcataccg caatgatgag ggaggcatgt
     1141 aaggaattac cgcagctagt ggagtatgtt cctttagcct gtgaagcgca tagccatcgc
     1201 agcgtcgccc ttgccaaaac cgtggctgca cccatcatgg cgacaggtgt gggcgcggct
     1261 ttaatgtatg caacatcatc aggaccgtat tatgcgcaaa gtctttctgg ttcgccggat
     1321 atcgagatga taaaaaaacg catggctgaa ttgtttaacc agtcggatac ttctgtgcgc
     1381 aacaaatatc gcccggcttt tgataaatgg aataaactgg tagataaatt aaatcacgaa
     1441 agaaatacag ttccgtttgc gtgtttgacc accattatag cgacagcgat aaatgaaact
     1501 cctgaggggg atacgaatgt tgccgttgtg atggggtgta aatctgcgaa agatcgcacg
     1561 atatccattg tccttggcaa ctcgatgttg caaacactct ttgaaaaacg cctcgccgat
     1621 gatggagaaa tagaaaacct ttttgatcaa cagggatatt ttagttgcga ttcattaaca
     1681 gccgaagaag taatgatgtt aaaagacctt tttgatattc gagttcttca tctctctaac
     1741 aaatttaatg ttgggttgca aggtaatatc aacacggatg ttttacaaga ttcgtttttt
     1801 aaaaatgtcg atttcattca tgaatctaac tcgtataccg tagggatggg agtctgaaga
     1861 gtaaaactca ttaggattat cagaattcgg ccaatgcatt gcaaaaatga aatacaaaga
     1921 gagatgtgtg caggcgctta gccgcgaggg gcatggcgaa gccatacaga aagcaccggc
     1981 attcagaact cctgtgaaag cggcatagtg ccgctgctca ggagttttgt tatgacccgt
     2041 atcgtgctga acgttgccac cctatgatgc gattaaaaaa actcaccgtc gagccgatga
     2101 ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg aaggaattcc ttgtttcccg
     2161 aactgttacc aacaaaatta taacgatgag caagtaaaga ccggttggtg gcagtactga
     2221 ccggtctgtg atggaagatt aatttcacct taaatcctgg tgctgttagg atgctgacgt
     2281 ttttcgggta caccattcag attatgcaaa taaagattca atgaaaaaat aaatatccct
     2341 gaatataata tattcgtagc acccataact catcctgggc gataactaaa ccatttttga
     2401 gtaacaaatg aaacaactgc aacgctctta ttatttctgg aaaacgtctc gcaaaaacaa
     2461 aacaacatgc aaaaatccgc tttcgttaat taatttttcg gcgtaatttt cgcacataca
     2521 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
     2581 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
     2641 gcaattttac gcgaagcggt agagcaatat ctggaacgtc agcaattccc ggtgcgtaaa
     2701 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
     2761 aaggaatgac aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
     2821 taatagaagg ctgttttact tgaacttata ataactgcaa ctgttacatc atatctggaa
     2881 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcgacaagg
     2941 gtacttttct ctccatagat atcaaatgat attggtcaaa tatttatgaa ctctctggca
     3001 ggtattgata tgggcagaat tatactcgat ttatcgaatg aggtgattaa gcaacttgat
     3061 gatcttgagg tgcagcgtaa tcttcctcgt acggagctat tacgggaagc ggtagatcaa
     3121 tacttggtaa atcaatcgca aacagtaaga accagtgctt ttggcatatg gcaagggtgt
     3181 gaggaagatg gtgtcgaata ccagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg
     3241 atatcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct
     3301 tacccggcct acaaagtcgt ataaattcaa tgtattgcag tttattggta ggcctaataa
     3361 gcgcggcgca tcaggcaatc ttacgcttat catcagtctc atacccataa ccttattgat
     3421 gtgggggtgg gtaaattgag gcaaaacagg cactggcggc atagcttgag gtggtaacgc
     3481 ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga ccagcgccac
     3541 ccggaaaacg tcagagatta ctccccttca cccggaaaca ggaacgggtt gatggaactg
     3601 cgggcatagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag gtcatctgcc
     3661 acggcttcaa ctttcggttc tttttcctga tagagaattt tcaggtaagt gccgcagtca
     3721 tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg
     3781 ccgctctgct cgcagttgct gcattttacg cgcacaacgt gccattcagt ttcacacagg
     3841 ttgcagtgca ggtaacgtag cccctgagtg gtgccaattt gcaccatgct ggacaccggc
     3901 atagaaccac aaaccgggca atattgacgt tgctcgccgt attcagcgcg ggctttgccg
     3961 gggatcagat tggccatctg cgcccagtag agcgacagtg cagcccagat aaacggcgct
     4021 ttatcgctgc tgacggacga gaaatcagag gcaaacagtg cgctggccat atcttccagc
     4081 tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc gctcatttca
     4141 ggtttcagct cagcaatcag cgccatcagc agcttttgcc agtgcttatc acgcggcaga
     4201 acgtgaatat ccagcggggg cttgccttgt gcgctggctt ctttaatgcg cgcagtcaga
     4261 tccatctcca gcggatggtc gtacagcacc acttcctggg cgtgggcgat aagcgcagca
     4321 aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg ctcggcgcgg
     4381 cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc cgccgtacgt
     4441 ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca gctttctttt
     4501 ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc caagcgctgg
     4561 taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat
     4621 gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt aacgcgaatc
     4681 ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc acgccgctca
     4741 ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga ccgaagttat
     4801 aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc ttcgcccaaa
     4861 agatatcatc ccgattgatt aggttgtgat gccagtaacg gaaaaacatg atgatgaacg
     4921 aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc ggtgtgccca
     4981 tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc accgccgcca
     5041 ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg tagcgcacga
     5101 tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat tattctcttc
     5161 ctcatccgca tggttcggac cgacacccac gtagtggaag atactggctg cgaaggtagc
     5221 cgcaaagcca acagctgcga gcggtttcca gatgcctttc cagaatttca cggtttcgct
     5281 gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat ggtgcagcac
     5341 gtacataacg tgtgtaccac cgacgcctgc cggatcgtac aggcccgcat tgtcgtaacc
     5401 gcgggttttc agctccgcca cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc
     5461 gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc caaccaccac
     5521 gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga ggcgcggaat
     5581 gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg actggaagtc
     5641 gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt tcagacagcc
     5701 tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt tgtcgttctg
     5761 ctccacttcc gagaagcgca tcaccgtcca cgatttggcg cttaaatcat tggggttgtc
     5821 gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg aacacgccac
     5881 ctgacaggct ttacagccga tacaggtggt gacgtcgatg agtttcgcca cttcttcctg
     5941 gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac gaatgatatc
     6001 ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca caaggaagga
     6061 cttaaactcc ggcgtctgcg tgttcgcatc accgacgaat ggcgtcaacg tattggcaat
     6121 aaagcctttt ttcgcaacac cttcatagcc ccagtgaata ggaataccga tggtatcgat
     6181 atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt tggctttgat
     6241 atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa gtttattcgc
     6301 cagcgactcc ccgatttcca caaactgctc tggttgcaaa atcgcgttca acagcgcgtg
     6361 tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat acgggaactt
     6421 atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat
     6481 aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt agtgttccgg
     6541 gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt ccggctgcat
     6601 gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa tatcccagcc
     6661 ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg ggttaccctg
     6721 cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg cccatgccca
     6781 acccagcgtg ttaccgaggc cagacggatc ggcgttatca cggcgtgcca tctggttgcc
     6841 ttccggcgtc cagctaccgg cgaaaatcca gcaaccacag gaagttgtac cgtcatcgcg
     6901 cagttgggca aacgagctaa gttgttggcc tttcttgacg ataaccgcac cggttgccgg
     6961 atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt ccgacttcgg
     7021 ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tggtccgggt tcgcgccacc
     7081 ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct cgccatcagt
     7141 cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt
     7201 aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca cttcggtctg
     7261 gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt tagaggtttc
     7321 agtgttcagc gggtcgatgg tgacgaggaa cttcagtttc gacagacaac tgatcacttt
     7381 gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc cattgacctt
     7441 gccttctttc atcatctcga agtattgcag gacgtcgtag cctttatccc acttcggcaa
     7501 ccagtcaaag ccccagctat tttctgccgt cgctttatca ccaaagaagg ccttcatcat
     7561 agagacgaag aatttcgggt agttgcccca gtagtttccc tggccttcca gcagcggttt
     7621 tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat
     7681 gtaacctggc aggctctgcg acagcagccc caggtccgtc agcccctgaa tattggagtg
     7741 accgcgcagg gcgttaacgc cgccgccagc catccccatg ttaccgagca gcagctggat
     7801 catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc cgagggcata
     7861 caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc agactttcag
     7921 gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac
     7981 gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat cgcgtttggc
     8041 gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc gtttttccgc
     8101 gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga tcaggctggc
     8161 gttggtatag gcttcagtgt attcgcggtt gaatttttca ttgttcagca ggtacagcaa
     8221 tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt agtcagccac
     8281 cgcagccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccat tgtgaatttt
     8341 ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac cgcccatcac
     8401 cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa
     8461 tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc
     8521 gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt tactcgacgc
     8581 ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt cggcgttttg
     8641 cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat caaacgcctc
     8701 ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg gaaacttcag
     8761 acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg
     8821 gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg cgttttttgc
     8881 tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg tgcaggtatt
     8941 acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg ctacgctggg
     9001 tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa
     9061 ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac
     9121 attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa
     9181 ggtccagaat tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat
     9241 gttggtgtgt gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat
     9301 cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc gtgatgattt
     9361 acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg tctacaacgg
     9421 catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt
     9481 ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc
     9541 ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta tggggttgaa
     9601 ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg tggagcaact
     9661 taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg atctcaacaa
     9721 actggatgat gcattacgtc atctcaacga ttttcagcca gtgggacaac tgaccggttg
     9781 tactcacgcc gccgcctgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt
     9841 gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg
     9901 gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc aaaagtcggc
     9961 gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt
    10021 ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg caacggttta
    10081 tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata tattaaattg
    10141 caggacttat tcatttcgtg aattttatta ttttatttat aaacaaaatg cgtgtagtgg
    10201 cgttttcttt tttcattt
//
LOCUS       DAZGXS010000146        10052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGXS010000146 DAZGXS010000000
VERSION     DAZGXS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1068..1598)
                     /gene="yodB"
                     /locus_tag="ACHKG5_004010"
     CDS             complement(1068..1598)
                     /gene="yodB"
                     /locus_tag="ACHKG5_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0870010.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1941..2591)
                     /gene="zinT"
                     /locus_tag="ACHKG5_004011"
     CDS             complement(1941..2591)
                     /gene="zinT"
                     /locus_tag="ACHKG5_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP0870011.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2848..3483)
                     /gene="msrQ"
                     /locus_tag="ACHKG5_004012"
     CDS             complement(2848..3483)
                     /gene="msrQ"
                     /locus_tag="ACHKG5_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0870012.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(3484..4488)
                     /gene="msrP"
                     /locus_tag="ACHKG5_004013"
     CDS             complement(3484..4488)
                     /gene="msrP"
                     /locus_tag="ACHKG5_004013"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0870013.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4596..5009)
                     /gene="hiuH"
                     /locus_tag="ACHKG5_004014"
     CDS             complement(4596..5009)
                     /gene="hiuH"
                     /locus_tag="ACHKG5_004014"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0870014.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5142..5813
                     /gene="hprR"
                     /locus_tag="ACHKG5_004015"
     CDS             5142..5813
                     /gene="hprR"
                     /locus_tag="ACHKG5_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP0870015.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5813..7171
                     /gene="hprS"
                     /locus_tag="ACHKG5_004016"
     CDS             5813..7171
                     /gene="hprS"
                     /locus_tag="ACHKG5_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0870016.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7279..8130)
                     /gene="hchA"
                     /locus_tag="ACHKG5_004017"
     CDS             complement(7279..8130)
                     /gene="hchA"
                     /locus_tag="ACHKG5_004017"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0870017.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8722..9908)
                     /gene="ompC"
                     /locus_tag="ACHKG5_004018"
                     /pseudo
     CDS             complement(8722..9908)
                     /gene="ompC"
                     /locus_tag="ACHKG5_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2796 a         2058 c         2298 g         2900 t
ORIGIN      
        1 attacaatta tcatgagtgt ttgtcaagtg attatatgag aaagacttgg tttattgttg
       61 attaatggta tgacctccga ttttcattga tagcttatgt gaaattcata ttgtgcttga
      121 ctttcgaagt gattgagctc agtgacgaga tttattggaa ttatagttaa acagaagaga
      181 ggttaaatac cgtggagagc tcgagttata caaggcgtta acgtgagcaa taaataggat
      241 gcgaagtaat tttggtggct cggaaaaggc gttttctgtc gttatcaatt gttcgggtgc
      301 agttttattt ttaacctgtt tgcttggaat attctgaggt tgtttgatgc taaccatctg
      361 atttttcaat cctaaatata gaatatttat tccaaataaa acacataata tattgatttt
      421 attaacccat agcataggtc tcgaaaaccg gagtaggggc aactctaccg ggggttcaaa
      481 tccccctctc tccgccactt tatcaatgac ttatctcccg acttcccgcc ttgcttttcc
      541 taaacagaac aatcgtagaa tattcttgaa gggttagatc gtcactgttt tctgttcgat
      601 actgtgacat tcagcacttg attcgctatg gatctgacag gaagctttcg agcgaaaatg
      661 tgcagttatt cagtcgtttt cttatcgctc accattattc ttttagacat tcatcctatg
      721 aaactgccgc aaagttggtg gtgggaactg aagttgcgta gagaaggggt caagaattga
      781 gtcaaaaaag gaacgcggcc gtcagattaa agccgcggga caaagtccat aatttccaag
      841 agttgttgaa tttcccagct tttttgaaag cctttgtatc atactaacaa gtaaaaaagg
      901 tttaaatacc aaaattaacc tgtatttttg ataagtctta ccattgtacg atttacacat
      961 tttctgctcc tccttgttga tagtggaaag agtgcggtat gcacttgcgt aaacatttat
     1021 gatgcgttga tgtccatggt tcacatttcc tttgtcagag gatcgactta tttgcggcga
     1081 ggcatcattc gtaacagagt attatctttc tgaatgtagt gatgaaagag tgctgcgcca
     1141 gcgtgagcta cgattaaaaa gtagcctata tttgcccagg tttcgtgaat attttttatc
     1201 agtgctttaa tttcgctgtt tggggtaaca aagggagaca cattgaaacc aaggaaactc
     1261 cacgattttc cactgtaagc catcaaagca atccccagca gaggaagcgc aaggaaggtt
     1321 atataaagca tgatgtgcat tagcgaagcc gctttcattt gccaggcagg tggcggtggc
     1381 acaatagaag gatcactata acggtgtttt ataatcaggc gtgaaaacat taacacccaa
     1441 acgaatatac ccgcattgta atgtgtttct cgcatcagca gataagtact actaccttta
     1501 ggaaaccagc cacggagttc catcgcggca taggttattg caacgaaaag cagcgttatc
     1561 caatgtaaat aaatttgagt ttttgagaat cggttcattt ttgtagctcc tgtaattttc
     1621 acagtcaagc tatcccacga accttaaaaa ttccttatac agatgtggtt ttaagaattt
     1681 gttatttaaa aaattgatag tgcacattgt aaccagccaa tataagcaat ggcgtgagca
     1741 caggtatctt tctctgttgg ccgtattgtg tatggaatcc gttattggtt aactcaatgc
     1801 gggttagctt attaatatac agacacgtca tagtgatgag cgtgtctgta gctagcttgc
     1861 gttcagtggc gtttgtggat ggaccagtgg cagttatgat gacatactaa agcggcatcg
     1921 aggcgttatc atgagaatac tcaatgagac atcatttcct cgaccacttc ttcgctactc
     1981 aactgatatg gataatacgt tggccagttt tccatttcat ttaataaaga ttgctgtgaa
     2041 tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat atgatcgcta
     2101 aattgtatat atttaggggc tttgctttca ggatctttac attcgaataa gtaacgaacg
     2161 cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata tttacaggat
     2221 gttgtttcat tatttctatg gaattcaaca atgccgtcct caatgccaat catctcgata
     2281 tctgttgcat agcctttgtg ataataatct ttaatttcag caaatgtttt agttttatct
     2341 gcatccgctt tcttctgaaa gacgggggca agtttgccac tttgcagtaa aggataaacg
     2401 gattgccaga ctccatccca gtcactgagc gttcggtttt gtacattggc atcatcaaaa
     2461 acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga gtgatgacca
     2521 tgcgaaaagg caggagcgct aacaataaag acacctaaag caacagccag tttgtgaaga
     2581 cgaatcgcca aaacagtttc ctccagagtc attgtatatg atgtgtaatg ttatattgta
     2641 acatatgaca aaatgcaaca actcttagga gatgagagca tagttttctc aggtgtactt
     2701 atctatctta ttgaaataaa atgaattatg ttgataattt gaggttgatg acaaacgaaa
     2761 cataacctga tgtgatctgc gtaggccgga tagggcgctc gcgccacatc cggcagggat
     2821 aacgcgtact ttgtcggcaa tatgaagtta tacagataat ttgttgtgaa cctgcttgcg
     2881 taaccggtta aaaagggaac gcgacttctt ataccgtaag gctaaaagca gtacagccag
     2941 cccagcgtag atgagcggct gcggtgaaat aatcttcact gaccacagat aatgtattgg
     3001 ggccaggatc gcgacaagat agacgaaatt atgcaactgt tgccagtgct tgccgagttt
     3061 tcgctgcatc gcctgggttg aagtaaacgc taaagcaagc aaaataatcc agctgataat
     3121 tcctaacgtt aaataaggcc gggtaattaa ctccttgcct aataacgcca gattattcac
     3181 gcccagctcc agcaatgcgt aactggttaa atgcagtgtc gcccaggcaa agcaccataa
     3241 gcctaacagg cggcgtgtgc gtatcaataa cggctgtttt gcgtagcgtg ccagaggtgt
     3301 gattaacaag gtcgccagca aaaatttcag tgcagtgcga ccggtaaaat gctgaatatc
     3361 tttcaccgga tcggcaccca gtccaccgtg attgatcgcc cagaccagcc agagaaacgg
     3421 caacaatccg gcaagatgca ggcaaacttt cagccatgtc acctgttttg ctgtcagacg
     3481 cattcagaaa ttctcccgca aatccaggcc acgatacagc gatgccacct ggtcggcgta
     3541 accattaaac agtagcgttg gctggcgctg tacatcgagg atgccgcctg aaccaataaa
     3601 tcgttcggta gcctgtgacc agcgtgggtg atcaacatgc ggattaacgt tggcgtaaaa
     3661 accgtattcg tcaggcgctg ccagattcca ggtggttggc ggacgctcgc gggtcagctt
     3721 aatactgacg atcgatttaa tccctttaaa gccatatttc cacggcacaa tcagtcgcac
     3781 cggcgcgcca ttttgtggcg gtaacgcctt gccataaaca cctacggtca tcagtgtgag
     3841 cggatgcatt gcttcgtcga gacgcaatcc ttcgacataa ggatatttca gcccgccgcc
     3901 gataaagcgg tcctgctggc ctggcatctg ttccggtgca taaattgttt cgaaagcgac
     3961 atacttcgca ttgctggtgg gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat
     4021 ccacggcacc accatcgacc acgcttccac gcagcgcata cgataaatac gctcttccag
     4081 cgggaagcga cgggttaaat catcgtgatc gagggtcaat ggttttgcca cttcgccgct
     4141 gattttcagt gtccatggat cggttttcag gctaccagca ttagcggcgg gatcggcttt
     4201 atccagcccg aattcataga agttgttata accggagact ttatctgctg gcgtcagtgg
     4261 caggttattt tgccaggcgg caggcttgct gaactccagc gcttttccgg cgggggcggg
     4321 tgggcgatcg ttccctttaa accagctaag cagatcggca tgcgcagcgt gaggcaaaga
     4381 aagtgcagct gcgctgatgc ccagtgcttt taacacctgc cgacgcttca taaagaatac
     4441 cgactcggcc gtaacatctg attcttttaa aaattgattc tttttcataa gccatcacac
     4501 tttttgtcgt tagccagcaa gagccgttta ctgttaatct gtcagggagt gtcctcccta
     4561 acagtagcag agatctgctt ttacatattt atgcattaac tgccacgata ggttgaatac
     4621 ccatattggc taagtaataa aggcacatga taatgctcgt tcactttatt aatatgaaat
     4681 tcaacgggga tctcagggaa gaaactttca agattttgtt tcttgaaata gtccccggtt
     4741 ttaaatacga cacggtaatc gcccgtagtt gcagtttgct cgggccacag tgccttaatt
     4801 cgtccatcct tatctgtttt ggcggtatta agttgtaacc agccgttgtc cgctttcttt
     4861 tcaagagtga ctgtcacgtc ggcagcgggt tttcctgttt gctggttcaa aatgtgcacg
     4921 ctaagaatgt tttgttgtgc agcatagacc aaagaaggta atgaaaatgc agccgttact
     4981 acggagagta ctaaataacg ctttaacatg tttatatcct tgtcatgtga atgagttttg
     5041 aggatagcga aaaagcgtcc agggtaacct tacagcagca ttacaatttt gtaatgaacc
     5101 aggctgtttc tatgacatat gatttatggc atattatttt catgaagatt ttacttattg
     5161 aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca
     5221 ttgatgccgt ttctgatggc agagatgggc tttatcttgc gctgaaggat gattatgcat
     5281 tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta caaacgttaa
     5341 gaacggcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc gatgacagag
     5401 tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca ttttctgagt
     5461 tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca acattagaaa
     5521 tcagcggctt aagaatggac tctgttagtc aaagtgtgag cagggacaat atcagtatta
     5581 cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct ggcgaaatta
     5641 tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt gataccaata
     5701 cggtggatgt cgccattcgc aggctccgcg caaaagttga tgatcctttt cctgaaaagc
     5761 taattgccac aatccgtggg atgggatatt cgttcgtagc ggtaaaaaaa taatgaaaag
     5821 attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg ttgctggcgc
     5881 cggaattgtc tggactctct ataatggcct cgcaagtgag ttgaaatggc gcgatgatac
     5941 aacactcatt aaccggacag cgcagatcaa gcagttatta attgatgggg taaatccaga
     6001 tacgttacct gtatacttta accggatgat ggatgttagt caggatatct tgatcattca
     6061 tggtgatggc atcaataaaa ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa
     6121 catacctgct agtgagacaa tcagcgcagc tggcatttac agaagcatta ttaatgatac
     6181 agagatagat gctttacgaa ttaatattga tgaagtttcg ccatcattaa cggttactgt
     6241 ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatca atagcattat
     6301 aatttgcatt gtcgccatta tactgtgctc agtattaagt ccgctgttaa tccgaacggg
     6361 gttacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata acgatagccg
     6421 ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
     6481 taaaatgcat catgctttag tcaaagattt tgagcgtcta agtcagtttg ctgacgatct
     6541 cgctcatgaa cttagaacgc caattaatgc attactgggt cagaatcagg ttacgctcag
     6601 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg aagagctgga
     6661 aaatatttcg cggttaacag agaacatact gtttcttgcc agggcagata aaaacaatgt
     6721 tttggtgaaa ctggactcgc tttctctcaa taaggaagtc gaaaatttgt tggactatct
     6781 tgaatatctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc agcaaatctt
     6841 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaacg ctattcgata
     6901 ttcgccagaa aaatcgcgta ttcatataac cagttttctt gataccaaca gctatcttaa
     6961 tattgatatc gccagccctg gagcgaaaat taatgagcct gaaaaactct tccgtagatt
     7021 ttggcgggga gataattcgc gtcattccgt aggtcaggga cttggtcttt ctttagtcaa
     7081 agcgattgcc gaattgcatg ggggaagtgc tacgtatcac tatctcaata agcataatgt
     7141 gttccggatt acgttgccgc aaagaaatta acatagggct tcagtacgcc ataaattaac
     7201 cgtgaaaaaa cgcagccacg tcgtgagtac taacgcggct gcgattgatt atgcgcttac
     7261 attcaaacgt aacagggatt aacccgcgta agctgccagc atttcctgcg ccgccagttt
     7321 acccaacgca ttcgctgcaa aaggactgtc gccggtgaga agtttacggt ccttatgtac
     7381 tcgcccggtg atgtcgtcat taatgatgtt catgcccatt ttcttcagtt cttcgccgaa
     7441 gtaccaggtg agatgacccg gcatatagcc aatctctggc gtttgtttgt ctgcggcgtc
     7501 tgggaatgcg caaatggaat aaccattcag tggattatcg ccgtggcgaa gcgccagaaa
     7561 agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgtaa
     7621 agcggcagct acgtcctggc tttcaggtaa accaataagt gcgccatgac caccaggaac
     7681 aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttctt
     7741 cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt catctttctg
     7801 cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc
     7861 gaaacctgca gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt
     7921 cgagaacagt tttccgttat cggtcggcaa ataacgttcg tccgcggcga tcaccagaat
     7981 tttatgttta ccgcgataag gttttggata gtccacgcca tcaagatcag agacaggact
     8041 ggtatattgg ctaagcgaat attctgaagg gaagaatgca ttatcttcag caatatcgac
     8101 ctgcggattt ttactggttt gaacagtcat agtgtattcc ttattgttgc ttagttaggg
     8161 tagtcactat atttgcgact gagctggtcg ctatttttaa ccaggctgga aaattgagtc
     8221 aatatatcga aagcggtagt cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt
     8281 tgaatattat tcacgccaaa ttttccttat tgagcaactt cgtcgcaagt ggcctcaatg
     8341 attttagacc attatcagag gtacggggcg gcggggagag atttagtgct gtggaatatt
     8401 gtggtgctga tgaatattta tatcgcggat tggtagagaa aaagcttatt tcatgcacta
     8461 tttttgcaaa tatttctata tgggaagcgt gtttgttacg gattaattca taaagaaatg
     8521 catttgggtg ttggcttgaa gagtcgctgg ttgatgttat tgtgccagga ggggcagggt
     8581 aagggggtgt atcgataagc tttggcaatt gcgtgtgccg cagatggaga tgaaatgcca
     8641 ataaaaaagc ctgagttcga tgagggacga actcaggcta gtgttcagtt ttacaataac
     8701 acgccgcggc gcgttggctg attagaactg gtagactaag cccacagcca cgatatcatc
     8761 ggtagcgatg ccgttttctt tgtagaagtc gtcgtcgttg tccagcaggt tgattttgta
     8821 atcaacataa gtggacatgt ttttgttgaa gtagtaggtc ataccaatat caacatattt
     8881 aaccagatct ttgttggtgt aatgatgatt accctggctg tcgaagttat cactatttac
     8941 accgcctaaa tccttacctt tagattgcag ataagcgatg gacgggcgca gaccgaagtc
     9001 gaactgatac tgagcaacaa cttcaaagtt ttgggttttg ttagcgatgg catcagcatc
     9061 accgtaacca gtcatgttac ggggttcagc atacatagct gctaagtaaa cgttattagc
     9121 gtcgtattta gcaccaacgg tccatgcttc tgctgtttca ccgccagcag ttgcatcgaa
     9181 gcgatctcca taacgagtgt tatgagtacc tttgtgaacc tgattgtcgg tacggtcaga
     9241 gttagagtaa gcagcgccca gacttaaacc aaagtcaaag tcataggaag tggacatact
     9301 gaaccgtcgc cgttctcttt agcaagttta cgtccattac cgttacctga accttcttga
     9361 tcaaataatc cgcttttctc gttgttaccc tgatactgga gtgcaaagtt cagaccatca
     9421 acttgaccaa agaaaccatt gttacgatag gttgctacgc tgttagcacg accgttcatg
     9481 aagttgtcag cgccagcata ggtatctcca ccaaattctg gcagcatatc ggtccatgct
     9541 tctacgtcat atacgacacc gtagttacga ccatagtcga aagtaccatt ctgaccaaag
     9601 ccgaaacctg caaatgccag acgcgttgcg gtattaccct tgtcgctttc aggaccgttt
     9661 gcgccaatgt taaattccca ctgaccataa ccagtgaatt ggtcagcgat ctgagtttca
     9721 cctttgaaac cgatacgggc atatgacatg tcgccatcgc tgctagcatc atcagagaag
     9781 tagtgcaggc ccgctacttt tccgtacaaa tccagtttat tgccatcttt attataaatt
     9841 tcagccgcat ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt
     9901 cttttcatta tgtattccct tgtgattata atcttcatga atatatcaat aagtgctgtt
     9961 atccaaaaaa aaagcacatt tggatactat tctatgaagt tcattttatt ttaaagatta
    10021 catgcaacaa atatatttaa aagatttcaa aa
//
LOCUS       DAZGXS010000147         9937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGXS010000147 DAZGXS010000000
VERSION     DAZGXS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            296..1273
                     /gene="glaH"
                     /locus_tag="ACHKG5_004019"
     CDS             296..1273
                     /gene="glaH"
                     /locus_tag="ACHKG5_004019"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0870018.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1293..2561
                     /gene="lhgO"
                     /locus_tag="ACHKG5_004020"
     CDS             1293..2561
                     /gene="lhgO"
                     /locus_tag="ACHKG5_004020"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0870019.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2584..4032
                     /gene="gabD"
                     /locus_tag="ACHKG5_004021"
     CDS             2584..4032
                     /gene="gabD"
                     /locus_tag="ACHKG5_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0870020.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4046..5326
                     /gene="gabT"
                     /locus_tag="ACHKG5_004022"
     CDS             4046..5326
                     /gene="gabT"
                     /locus_tag="ACHKG5_004022"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0870021.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5564..6964
                     /gene="gabP"
                     /locus_tag="ACHKG5_004023"
     CDS             5564..6964
                     /gene="gabP"
                     /locus_tag="ACHKG5_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0870022.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6985..7647
                     /gene="csiR"
                     /locus_tag="ACHKG5_004024"
     CDS             6985..7647
                     /gene="csiR"
                     /locus_tag="ACHKG5_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0870023.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7648..8097)
                     /gene="kbp"
                     /locus_tag="ACHKG5_004025"
     CDS             complement(7648..8097)
                     /gene="kbp"
                     /locus_tag="ACHKG5_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0870024.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8181..8339)
                     /gene="yqaE"
                     /locus_tag="ACHKG5_004026"
     CDS             complement(8181..8339)
                     /gene="yqaE"
                     /locus_tag="ACHKG5_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0870025.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8522..8821
                     /gene="ygaV"
                     /locus_tag="ACHKG5_004027"
     CDS             8522..8821
                     /gene="ygaV"
                     /locus_tag="ACHKG5_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0870026.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8831..9349
                     /gene="ygaP"
                     /locus_tag="ACHKG5_004028"
     CDS             8831..9349
                     /gene="ygaP"
                     /locus_tag="ACHKG5_004028"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0870027.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9402..9806)
                     /gene="stpA"
                     /locus_tag="ACHKG5_004029"
     CDS             complement(9402..9806)
                     /gene="stpA"
                     /locus_tag="ACHKG5_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0870028.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2268 a         2546 c         2715 g         2408 t
ORIGIN      
        1 tatttagttt actagcggtc attttgcagt gaagccattt accgtttttt atcgaccaga
       61 taatctgttc tctgatggta atccccccct aacctgttgc tttagttatt catttcctgt
      121 ctcactttgc cttaataccc tacgttaaat gttaccaatt tgttgctttt gatcacaata
      181 agaaaacaat atggcgcatt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
      241 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
      301 tgcactgacc gccgtacaaa ataacgctgt cgattcagac caggactata gcggattcac
      361 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
      421 caaacagttt ctcgaacagg ttgccgaatg gcccgtgcag gcgctggagt acaaatcgtt
      481 tctgcgtttt cgggtaggta aaattcttga cgatctgtgt gcgaaccagc tgcaaccatt
      541 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtgt
      601 cgatgatgtg aagcaagcgg atgagatggt gaagctggcg acggcggtgg cgcatctgat
      661 tggccgttcc aatttcgatg cgatgagcgg tcagtattac gcgcgttttg tagtgaaaaa
      721 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtaatggagc tgcacaacga
      781 cggcacctac gtcgaagaga tcactgacta tgtgctgatg atgaaaatcg acgagcagaa
      841 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
      901 tttccgccat ccaatggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
      961 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     1021 ctatatcgac cagttcgtcc agccaaaaga cttcgaagaa ggcgtgtggt tgagcgagct
     1081 ttcggacgcc attgaaatca gtaaaggcat tctttctgta cccgttcccg ttggcaaatt
     1141 cctgttgatt aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     1201 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactatca
     1261 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     1321 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ctatccggat gcccgcattg
     1381 cgttgctgga aaaagagtcc ggcccggcct gtcatcagac gggccacaac agcggcgtga
     1441 tccatgccgg ggtttattac acgcccggta gcctgaaggc gcagttttgc ctggcgggaa
     1501 accgcgccac taaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     1561 tgctggtcgc cacctctgaa ctcgaaatgg aacggatgcg cgcgttgtgg gaacgcacgg
     1621 cggcaaacgg tatcgagcgc gagtggttaa acgccgatga actgcgcgag cgcgaaccga
     1681 atatcaccgg gcttggcggt atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     1741 tcacggcggc gatggcaaaa attttccagg ccagaggcgg cgagattatc tataacgccg
     1801 aagtcagcgg ccttagtgag cataaaagcg gcgtggtgat acgtacccgt cagggcagcg
     1861 actatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     1921 tgctcggcct cgaaccgggc tttattatct gcccgttccg cggcgagtat ttccgcctgg
     1981 cgccggagca taaccagatt gttaaccatc tgatttaccc cattcccgac ccggcaatgc
     2041 cgtttttggg cgtgcatctc acccgcatga tcgacggcag cgtcaccgtc gggccaaacg
     2101 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agcgacacgc
     2161 tggagatttt gggctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     2221 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct gcggctggtg caaaagtatt
     2281 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcgg
     2341 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccaccccg cgcacgatcc
     2401 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     2461 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     2521 cacgtagtgt ggatgcctta cacgccgcgt ttaatcaata acctttgaaa acaggatgta
     2581 gcgatgaaac tgaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     2641 ctggacgcca acaatggtga agtcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     2701 ggtagcgtgc cgaaaatggg cgctgatgaa actcgcgccg ctatcgacgc cgccaaccgc
     2761 gccctgcccg tctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     2821 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggt
     2881 aaaccgctgg ctgaagcgaa aggcgaaatc agctacgccg cctcttttat tgagtggttt
     2941 gccgaagaag gcaaacgcat ttatggcgac accattcccg gtcatcaggc cgataagcgc
     3001 ctgattgtga tcaagcagcc gattggcgtt acagcggcca tcacgccgtg gaactttccg
     3061 gcggcaatga ttacccgcaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     3121 aagcccgcca gtcagacgcc gttctctgcg ctggcgctgg cggagctggc gatccgcgcg
     3181 ggcattccgg ctggcgtgtt taacgtggtc accggttcgg cgggcgcggt gggtaatgaa
     3241 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gctcgaccga aattggccgc
     3301 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     3361 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcactggcc
     3421 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     3481 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcatatcggc
     3541 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     3601 gtggaagagc atattgccga tgcgctggaa aaaggcgcgc gcgtagtttg cggcggtaaa
     3661 gcacatgaac gcggcggcaa cttcttccag cccaccattc tggtggacgt gccggccaac
     3721 gccaaagtgt cgaaagaaga gaccttcggc cccctcgccc cgctgttccg ttttaaagat
     3781 gaagccgatg tgatcgcgca agccaatgac accgagtttg gccttgccgc ctatttctat
     3841 gcccgtgatt taagtcgcgt cttccgcgtg ggcgaggcgc tggagtacgg catcgtcggc
     3901 atcaataccg ggattatttc caatgaagtg gccccgttcg gcggcattaa agcctcgggt
     3961 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     4021 atcggtcttt aactggagaa cgcgaatgag cagcaataaa gagttaatgc agcgccgcag
     4081 tcaggcaatt cctcgtggcg ttgggcaaat tcaccccatt ttcgctgatc gcgcggaaaa
     4141 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg gcattgcggt
     4201 gctcaatacc gggcacctgc atccgaaagt ggttgccgcg gtggaagcgc agttgaaaaa
     4261 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     4321 tatgaatcag aaggtgccgg gcgattttgc caagaaaacg ctgctggtta cgaccggttc
     4381 cgaagcggtg gaaaacgcgg tgaaaatcgc ccgcgctgcc accaaacgta gcggcaccat
     4441 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     4501 gaatccgtac tctgcgggca tgggcctgat gccagggcac gtttatcgcg cgctttatcc
     4561 ttgcccactg cacggcatca gtgaagatga tgctatcgcc agtatccacc gaatttttaa
     4621 aaatgatgct gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     4681 cggtttctac gccgcgacgc ctgcgtttat gcagcgttta cgcgcgctgt gtgacgagca
     4741 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     4801 tgcgatggag caaatgggcg tggcaccaga tctcaccacc tttgcgaaat cgatcgcagg
     4861 cggcttccca ctggcgggcg tcaccgggcg cgccgaagtg atggatgccg tcgctccagg
     4921 cgggctgggt ggcacctatg ccggtaatcc gattgcctgc gtggcggcgc tggaagtgtt
     4981 gaaggtgttc gagcaggaaa atctgctgca gaaagccaac gatctggggc agaagttgaa
     5041 agatggattg ttggcgatcg ccgaaaaaca ccctgagatc ggcgacgtac gcgggctggg
     5101 ggcgatgatc gccatcgagc tgtttgaaga cggcgatcac aacaagccgg acgccaaact
     5161 caccgccgag atcgtggctc gcgcccgcga taaaggcctg attcttctct cctgcggccc
     5221 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     5281 gggtctggag atcatcagcc agtgttttgc tgaggcaaag cagtagcgcc gctcctatgc
     5341 cggaggcgac gctgcgcgtc ttgtccggcc tacggggatc caggtcggat aaggcgttta
     5401 cgccgcatcc gacaatctgt acgtgaacag gaataaatct atgttggccg ggtaaggcgg
     5461 agccgctctc cggcaaaaag aatcaataac aattatacgc gtgacccgac gcgggaaatg
     5521 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
     5581 accacatgag ttaggcggcg ggctgaagtc acgccatgtc accatgttgt ctattgccgg
     5641 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
     5701 ggtattactg gcctatctgt tcgccgggct actggtggtt atgattatgc ggatgttggc
     5761 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagctat
     5821 tggtcgttgg gcgggttata ccatcggctg gttgtactgg tggttttggg tactggttat
     5881 cccgctggaa gccaacatcg ccgccatgat cctgcactca tgggttccag gcattcccat
     5941 ctggttattt tccctcgtca ttacccttgc cttaactggc agtaatttat taagcgttaa
     6001 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
     6061 tattttcctt ggtgcagtcg caattagcgg tttttacccg tatgccgacg tgagcgggat
     6121 ctcaagattg tgggatagcg gcggctttat gcccaacggc ttcggtgcgg tattaagcgc
     6181 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
     6241 atccgacacg ccggaaaaac atattgtccg cgctaccaac tcggttatct ggcgtatttc
     6301 tatcttctat ttgtgctcta tttttgtcgt agtggcgtta ataccgtgga atatgccggg
     6361 gctgaaagcc gttggatctt atcgctcggt tctggaattg ctcaatatac cccatgcgaa
     6421 attaatcatg gactgcgtga tattactttc cgtaaccagt tgcctgaact cggcgctgta
     6481 taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgcgcccg cggtaatggg
     6541 caaaatcaac cgcagtaaaa ccccgtatgt ggcggtgtta ctctccaccg gagcggcatt
     6601 tttaacggtc gtggtgaact attacgcacc tgcgaaagtg tttaaattcc tgatcgacag
     6661 ctccggtgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     6721 taaaattctg cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt acccgtggct
     6781 cacctggctg gtaatcggct ttattacctt tgtgttggtg gtgatgttat tccgcccggc
     6841 gcaacagtta gaagtgatct ctaccggctt attagcgata gggattatct gtaccgtgcc
     6901 gattatggcg cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg
     6961 ctgataatga tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg
     7021 aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacgaat gagtttgctg
     7081 acatcgcgtt atgcacttgg cgttgggccg ttacgggaag ctctttcgca actggtggcg
     7141 gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag
     7201 gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgttagt gagtctggcg
     7261 attgcccgcg gtggcgatga gtgggaggca gacgttctcg caaaagcgca tctgctgagt
     7321 aagcttgagg cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag
     7381 gcgtttcata ctgcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa
     7441 cggttgtttg atctggcggc gcgttatcga tttatctggc tgcggcgaac ggtgctttcg
     7501 gtggaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg
     7561 cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt
     7621 atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca cttgccccgg
     7681 ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag
     7741 attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt
     7801 ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat
     7861 accggaaata ttccccaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc
     7921 accagtgacc gtcgctttgc catcggcaat ttgaatgttc actttatcgg catcaggtat
     7981 accggttttg ctcagatgct cctgcacctt cttcgcctga tcatctttat cgtgctgacc
     8041 tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc
     8101 tacctcattc gttaacggaa acactcagaa agtgtagtag aggaacgcgg cgcgttaacg
     8161 ccgcggggat gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg
     8221 gggatatacc caagcagtgt caacagaata ttaataatga acgcccaacc gaacccttta
     8281 ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca gaaacccata
     8341 tgtactccct ataagaaaat tacttattgt ttaaaaagag attttatctc ttaagcgtag
     8401 taattttagc ggaggctgcc agtttcagct tacgctttta ctctcattta cgctgctgag
     8461 gctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata cttaaataac
     8521 tatgaccgaa ctcacgcaat tacaggccag tgctgaacag gcagcggcct tattgaaagc
     8581 aatgagccac cctaaacggt tgctgattct gtgcatgctt agcggttccc ccggcaccag
     8641 cgcgggagag ctgacgcgca ttaccggact gagcgcctct gcgacatcac agcatctcgc
     8701 tcgtatgcgg gacgaagggc ttatcaacag ccaacgggat gcccaacgca ttctatattc
     8761 cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct attgtccgta
     8821 aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat taatcgcacg
     8881 cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat cttcgtgaac atattcctga
     8941 agcagatctg gctccattat ccgtgctgga acagtcaggt ctcccggcta aattacgtcg
     9001 cgatcaaatt atattccact gccaggcagg taaacgcacc agtaataacg ccgataaatt
     9061 agcagcgatt gccgcccccg cagaaatctt tttactcgaa gatgggattg atggctggaa
     9121 aaaagcagga ttaccagtag cggtaaataa atctcaaccc ttgccgttaa tgcgccaggt
     9181 gcagatcgct gcgggtggtt taatattaat cggcgttata ctgggttata ccgtaaatag
     9241 cggtttcttc ttattaagcg gctttgttgg tgccgggtta ctgtttgcag gaattagcgg
     9301 tttttgtggg atggcaaggt tgttagataa gatgccgtgg aaccaatgag cttgagaagc
     9361 gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag gaaatcgtcg
     9421 agagatttac cttctgccag cgcctgagcg attggcttcg gtgtacggcc ctgaccggtc
     9481 caggttttag tttcaccgtt aacatcggtg aatttatatt tcgccggacg cggctggcgt
     9541 tttttaccag cacgtggtgc agcagcagag ctattaccca ataactcttc cgggttaatt
     9601 ccgtcagctt tcatcagctc cagccaggtg ctaatttttt cctggcgctc tgccagttca
     9661 cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa tttttcgagc
     9721 atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagggt gcgaatgtta
     9781 tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca aaacaaaaaa
     9841 gtatttcgtc gcgtagtgta agcgatcact tattatattt ccacaaaatt taatctattt
     9901 acagtcctgc gcgagattat ttcagctgtt attttct
//
LOCUS       DAZGXS010000148         9864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGXS010000148 DAZGXS010000000
VERSION     DAZGXS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            101..175
                     /gene="tnaC"
                     /locus_tag="ACHKG5_004030"
     CDS             101..175
                     /gene="tnaC"
                     /locus_tag="ACHKG5_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP0870029.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            396..1811
                     /gene="tnaA"
                     /locus_tag="ACHKG5_004031"
     CDS             396..1811
                     /gene="tnaA"
                     /locus_tag="ACHKG5_004031"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0870030.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1902..3149
                     /gene="tnaB"
                     /locus_tag="ACHKG5_004032"
     CDS             1902..3149
                     /gene="tnaB"
                     /locus_tag="ACHKG5_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0870031.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3281..4456
                     /gene="mdtL"
                     /locus_tag="ACHKG5_004033"
     CDS             3281..4456
                     /gene="mdtL"
                     /locus_tag="ACHKG5_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP0870032.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4431..5390
                     /gene="yidZ"
                     /locus_tag="ACHKG5_004034"
     CDS             4431..5390
                     /gene="yidZ"
                     /locus_tag="ACHKG5_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0870033.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5547..6296
                     /gene="yieE"
                     /locus_tag="ACHKG5_004035"
     CDS             5547..6296
                     /gene="yieE"
                     /locus_tag="ACHKG5_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0870034.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6318..6884
                     /gene="yieF"
                     /locus_tag="ACHKG5_004036"
                     /gene_synonym="chrR"
     CDS             6318..6884
                     /gene="yieF"
                     /locus_tag="ACHKG5_004036"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0870035.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6938..8275)
                     /gene="adeP"
                     /locus_tag="ACHKG5_004037"
     CDS             complement(6938..8275)
                     /gene="adeP"
                     /locus_tag="ACHKG5_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0870036.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8441..9106
                     /gene="yieH"
                     /locus_tag="ACHKG5_004038"
     CDS             8441..9106
                     /gene="yieH"
                     /locus_tag="ACHKG5_004038"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0870037.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9243..>9864)
                     /gene="espY4"
                     /locus_tag="ACHKG5_004039"
     CDS             complement(9243..>9864)
                     /gene="espY4"
                     /locus_tag="ACHKG5_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HFP0870038.1"
                     /translation="PPDGTILTENPKEYVQKQIKESHKESATNVTFDYKELAPIFEGV
                     EEKQIKALSNQLEHIKSFKTDYDSRLNKYARDFYYFSSAFAINWEDLLKNYQEIRASI
                     KDYDDLLREIKELIISRNKSLEDKRTLFDNKRDNWNSIEVQDEVNALNAKIVDCDDKI
                     RSKLTIVRNNRLENQYKDDKNISDAMRNILDWFERYPDIVQNITQA"
BASE COUNT         2446 a         2417 c         2394 g         2607 t
ORIGIN      
        1 ataactaaat gtgattcgat tcacatttaa acaatttcat aatagacaaa tttcttgagt
       61 attatactgt agcttcgtgt ctttcgagga taagtgcatt atgaatatct tacatatatg
      121 tgtgacctca aaatggttca atattgacaa caagattgtc gatcaccgcc cttgatttgc
      181 ccttctgtag ccatcaccag agccaaaccg attagattca atgtgatcta tttgtttgct
      241 atatcttaaa tttgcctttt gcaaaggcca tctctcgttt atttacttgt gttagtaaat
      301 gatggtgctt gcatatatac atggcgaatt aatggatatt gcagatgcaa tattcacagg
      361 gatcactgta attaaaataa atgaaggatt atgtaatgga aaactttaaa catctccctg
      421 aaccgttccg cattcgtgtt attgagccag taaaacgtac cactcgcgct tatcgtgaag
      481 aggcaattat taaatccggt atgaacccgt tcctgctgga tagcgaagat gtgtttatcg
      541 atttactgac cgacagcggc accggggcgg tgacgcagag tatgcaggcc gcgatgatgc
      601 gcggcgacga agcttacagc ggcagccgca gctactatgc gttagccgag tcagtgaaaa
      661 atatctttgg ttatcaatac accattccga ctcaccaggg ccgtggtgca gagcaaatct
      721 atattccagt actgattaaa aagcgcgagc aggaaaaagg cctggatcgc agcaaaatgg
      781 tggcgttctc taactatttc tttgatacca cgcagggcca tagccagatt aacggctgta
      841 ccgtgcgtaa cgtctatatc aaagaagcct tcgatacggg cgtgcgttac gactttaaag
      901 gcaactttga ccttgaggga ttagaacgcg gtattgaaga agttggtccg aataacgtgc
      961 cgtatatcgt tgcaaccatc accagtaact ccgcaggcgg tcagccggtt tcactggcaa
     1021 acttaaaagc gatgtacagc atcgcgaaga aatatgatat tccggtggta atggactccg
     1081 cgcgctttgc tgaaaacgcc tatttcatca agcagcgtga agcagaatac aaagactgga
     1141 ccatcgagca gatcacccgc gaaacctaca aatatgccga tatgctggcg atgtccgcca
     1201 agaaagatgc gatggtgccg atgggcggct tgctgtgcat gaaagacgac agtttctttg
     1261 atgtgtacac cgagtgcaga accctttgcg tagttcagga aggcttcccg acgtatggcg
     1321 gcctggaagg cggcgcgatg gagcgtctgg ccgtaggtct gtatgacggc atgaatctgg
     1381 actggctggc ttatcgtatt gcgcaggtgc agtatctggt cgatggtctg gaagagattg
     1441 gcgttgtctg ccagcaggcg ggcggtcacg cggcattcgt tgatgccggt aaactgcttc
     1501 cgcatatccc ggcagatcag ttcccggcac aggcgctggc ctgcgagctg tataaagtcg
     1561 ccggtatccg cgcggtagaa attggctctt tcctgttagg ccgcgatccg aaaaccggta
     1621 aacaactgcc atgcccggct gaactgctgc gtttaaccat tccgcgcgca acatatactc
     1681 aaacacatat ggacttcatt attgaagcct tcaaacatgt gaaagagaac gcgtcgaata
     1741 ttaaagggtt aacctttacc tacgaaccaa aagtattgcg tcacttcacc gcaaaactga
     1801 aagaagttta attaatacta ctgagtggct ataaggatgt tagccactct cttaccctac
     1861 atcctcaata acaaaaatag ccttcctcta aaggtggcat catgactgat caagctgaaa
     1921 aaaagcactc tgcattttgg ggtgttatgg ttatagcagg tacagtaatc ggtggaggta
     1981 tgtttgcttt acctgtggat cttgccggtg cctggttttt ctggggtgcc tttatcctta
     2041 tcattgcctg gttttcaatg cttcattccg ggttattgtt attagaagca aatttaaatt
     2101 atcccgtcgg ctccagtttt aacaccatca ccaaagattt aatcggtaac acctggaaca
     2161 ttatcagcgg tattaccgtt gccttcgttc tctatatcct cacttatgcc tatatctctg
     2221 ctaatggtgc gatcattagt gaaacgatat caatgaattt gggttatcac gctaatccac
     2281 gtattgtcgg gatctgcaca gccattttcg ttgccagcgt attgtggata agctcgttag
     2341 ccgccagtcg tattacctca ttgttcctcg ggctgaagat tatctccttt gtgatcgtgt
     2401 ttggttcttt cttcttcctg gtcgattact ccattctgcg cgatgccacc agctccactg
     2461 cgggaacgtc ttacttcccg tatatcttta tggctttgcc ggtgtgtctg gcgtcatttg
     2521 gtttccacgg caatattccc agcctgatta tttgctatgg caaacgcaaa gataagctaa
     2581 tcaaaagcgt ggtcttcggt tcgctgctgg cgctggtgat ttatctcttc tggctctatt
     2641 gcaccatggg gaatattccg cgagaaagct ttaaggcgat tatttcctca ggcggcaacg
     2701 ttgattcact ggtgaaatcg ttcctcggca ccaaacagca cggcattatc gagttttgcc
     2761 tgctggtgtt ctccaactta gctgtcgcca gctcgttttt tggtgtcacg ctgggattgt
     2821 tcgattatct ggcggacctg tttaagattg ataactccca cggcgggcgt ttcaaaaccg
     2881 tgctgttaac cttcctgcca cccgcgttgt tgtatctgat cttcccgaac ggctttattt
     2941 acgggatcgg cggtgccgga ctgtgcgcca ctatttgggc ggtcattatt cccgcagtgc
     3001 tggcaatcaa agctcgcaaa aagtttccca atcagatgtt cacggtctgg ggcggcaatc
     3061 ttattccggc gattgtcatt ctctttggta taaccgtaat tttgtgctgg ttcggcaacg
     3121 tctttaacgt gttacctaaa tttggctaaa tccttcaaga agccagtcat tcgctggctt
     3181 cttgcctctc aggaaatcac ttatgtccaa atggcaactc gcctgatcct ccttcaccac
     3241 gtatgctttg cgtcacctta ctatcaggac gctttagccc atgtcccgct ttttgatttg
     3301 tagttttgcc ctggttttac tttatcccgc tgggattgat atgtacctcg ttggtttacc
     3361 gcgcatcgcc gccgatctca atgccagcga agcgcagtta catattgcgt tctccgttta
     3421 tctggcaggt atggcagctg cgatgttatt tgccggtaaa gtggccgatc gttcagggag
     3481 aaaaccggtc gccatccccg gcgcggcgct atttattatc gcttcggtgt tctgttcact
     3541 ggctgaaacc agcacgttat ttcttgcagg gcgctttcta caggggttgg gcgcgggctg
     3601 ttgttacgtg gtggcattcg ccattttgcg cgacacgctg gatgatcgac gtcgggcaaa
     3661 agtgctgtcg ttactcaacg gcattacctg catcattccg gtgttagcgc cggtgctcgg
     3721 tcatctgatt atgcttaaat tcccgtggca gagtctgttc tggacgatgg caataatggg
     3781 catcgcggta ctgatgttgt ctttgtttat tttaaaagaa acgcgccctg ccgcccccgc
     3841 agcttcggac aaatcacgag aaaatagcga gtcgctgctt aatcggtttt tcctcagccg
     3901 tgttgttatc accaccctca gcgtttcggt gatcctcacc ttcgtcaata catcgccggt
     3961 attgctgatg gaaatcatgg gttttgagcg cggagaatac gccaccatta tggcgttgac
     4021 cgctggcgtc agcatgaccg tttcattttc cacgccattt gcgctgggaa tttttaagcc
     4081 acgtacgttg atgatcacct cacaggtgtt attcctggcg gcgggcatca ctcttaccgt
     4141 ttcaccttcc catgcggttt ctctgtttgg tatcacgctg atttgcgccg gtttctcggt
     4201 aggttttggt gtggcgatga gtcaggcgtt agggccgttt tcattacgcg cgggcgtagc
     4261 cagctcgacc ttaggtattg cgcaggtttg cggttcgtca ctgtggattt ggctggcagc
     4321 ggtggttggt atcagcgcat ggaatatgct gatcgggatt ctgattgcct gtagcatagt
     4381 gagcctgttg ctgattatgt tcgtcgcgcc tggacgcccg gttaccgctc atgaagaaat
     4441 ccatcaccac gcttgatctc aatctgctgc tctgtctgca gctgctgatg caggagcgca
     4501 gcgtaaccaa agcggcgaag cggatgaacg tgacaccttc ggcggtgagt aagtcgctgg
     4561 caaagttaag agcgtggttt gacgacccgc tctttgtgaa ctcaccgttg gggctgtcgc
     4621 ccacaccgct aatggtcagc atggagcaaa atctggcgga gtggatgcaa atgagcaacc
     4681 tgctgctgga taaaccgcac caccagacac cgcgcggcct gaagtttgag ctggcggcag
     4741 aatcaccgct aatgatgatc atgcttaatg cgctgtcgaa acagatctac caacgttacc
     4801 cgcaggcgac catcaaatta cgtaactggg attacgattc cttagatgcc attactcgtg
     4861 gtgaagtgga tatcggtttt tccggtcgcg aaagtcatcc ccgttcgcgg gagctgttaa
     4921 gctcgctacc gttagccatt gattatgaag tgttgtttag tgatgtgccc tgcgtctggt
     4981 tacgcaaaga tcatccggca ctgcatgaaa agtgggatct cgataccttt ttacgttatc
     5041 cgcatatcag catttgctgg gaacagagcg atacctgggc gctggacaat gtgttacagg
     5101 agctggggcg cgaacgtacg attgcaatga gcctgccgga attcgagcag tcactgttta
     5161 tggcagcgca gcccgacaat ctgctcctgg cgaccgcgcc gcgctactgt cagtactaca
     5221 atcaactcca ccaactgccg ttggttgctc ttcctctccc gtttgacgaa agccagcaaa
     5281 aaaagctgga agtccctttt accctgctgt ggcataaacg gaacagccat aatccgaaga
     5341 tcgtctggtt acgggatacc attaaaaatc tttacgcgtc gatggcataa ccgaatcgta
     5401 tgaaacgcga cccgatttca caataaaatg taaaaaagtt gtaataagct tgtctgaatc
     5461 gaacttttag ccgctttagt ctgtccatca ttccagtaaa tgattactct tgtattcata
     5521 atggaccatt aagcatggag cgaaaaatgg cgactcactt tgcccgaggg attttaacgg
     5581 aaggacatct gatttctgtt cgtctcccct cccagtgtca tcaagaagcc cgaaacattc
     5641 cccctcatcg tcaaagccgt tttctggcgt ccagaggttt actcgcagaa ctgatgttca
     5701 tgctgtatgg cattggcgaa ttgccggaaa tcgtcaccct gccgaaaggt aaaccggttt
     5761 tcagtgataa aaatttgcct tcgttttcta tttcctatgc cgggaatatg gttggcgtgg
     5821 cgttaacaac cgaaggtgaa tgtggcctcg atatggaact gcagcgtgcg acgcgcgggt
     5881 ttcatagccc acacgcgccc gataaccaca cattttccag caatgaatcg ctatgggtca
     5941 gtaaacaaaa tgatccaaac gaagcgcggg cgcagctcat cacgctgcgc cgaagcgtgc
     6001 taaaactaac cggtgatgtt ttgaatgacg atccgcgcga tctgcaactg ctgcccattg
     6061 ccggacgcct gaaatgtgct catgtaaatc atgtagaagc gttatgcgac gcggaggacg
     6121 tgctggtgtg gtccgtggcg gtcacgccca cgattgaaaa gctcagtgtc tgggagttag
     6181 atggcaaaca cggctggaaa agcctgccgg atattcacag ccgcgccaac aatcctacca
     6241 gccggatgat gcgttttgcc cagctctcta ccatgaaggc tttttcgcca aattgataga
     6301 caaccacagg agtcattatg tctgaaaaat tgcaggtggt tacgttactg gggagcctgc
     6361 gcaaaggctc atttaatggc atggttgcac gtaccctacc gaaaattgct ccggcgagca
     6421 tggaagtcaa tgcgttacct tccattgccg acattcccct gtatgacgct gacgtacagc
     6481 aggaagaagg ttttccagca acggttgaag ctctggcgga acagatccgt caggctgacg
     6541 gtgtggtgat cgtcacgccg gaatataact attcggtacc gggtgggctg aaaaatgcca
     6601 tcgactggct ttcccgcctg ccggatcaac cgctggccgg taaaccggtg ttgattcaga
     6661 ccagctcaat gggcgtgatt ggcggcgcgc gctgtcagta tcacctgcgc cagattctgg
     6721 ttttcctcga tgcaatggtg atgaacaagc cggaatttat gggcggcgtg attcagaaca
     6781 aagttgatcc gcaaaccgga gaagtgattg atcagggtac gctggaccac ctgaccgggc
     6841 aattgaccgc atttggcgag tttattcagc gagttaagat ctaaataaaa aacccgccag
     6901 caatcatgca tggcgggttt ttaacgcgct atcgatttta gtgagcgtcg ataaatacaa
     6961 tcttcaggat aaacagcagc gcaacgatga ttacgcacgg gctaaggtca cgaatacgcc
     7021 cggtaccaat cttcatcacg cagtaggaga taaagcccag cgcaatacct tcggtaatcg
     7081 agaagctgaa cggcatcatc acagcggtaa taaacgccgg aacagattca gtaagatcct
     7141 gccagttcac gcgagcaaga cttgaggtca tcaacacgcc aacgtaaatc agcgcgccag
     7201 ctgcagcgta gcctggcacc attcccgcca gcggcgacag aaagataacc agcaggaaca
     7261 gcagaccaac aaccactgcc gtcagaccgg tacgaccgcc aaccgatacg ccggaagagg
     7321 actcaatata agccgtaacg gaagaagtac cgataaacga accggtcacg gaagagatac
     7381 tgtcgacata cagcgcctgc ttcatgcgcg ggaatttccc cttctcatct gccagaccgg
     7441 ctttatcggt cacgccaatc aacgtgccgg aggagtcaaa caggttgacc agcatgaaag
     7501 agaaaatcac ccctgccagc ccgaggttaa acgacccggc taaatctaca tgacccacga
     7561 cagtcattac gctcggcggc gcagaaacga tgccattgta gtggacatca cccagcatcc
     7621 agcccagcag cgttgtcacc acgatagaaa ccagcaccgc tgcgtgaatg ttgcgtgagg
     7681 ccagaatagc aatgatgaag aagccgagga tccccagcag cacgctgtga gaagtcagat
     7741 taccgatgct gaccagcgtt tccgggttag cgacaatcac acctgcgttt ttcagcccca
     7801 tcatgccaat gaacagaccg ataccgctgg taatgcccac acgcagactc accggaatgt
     7861 tggcaatcat ccagtagcga acgcggaaaa tcgtcagtaa cagcagacca atcgcgcccc
     7921 agaagattgc gcccatcccg acttgccacg gcaagcccat cgcctgcaca acgacaaaag
     7981 cgaagaacgc attcaggccc atagcgggtg ccagtgcaac tggcaggtta gcaaacagcc
     8041 ccatcataat actgccgaat gcagcaatca gacaggtagt gacgaagacg gcgctggtat
     8101 ccatgccagc aacgccaaga atttgcgggt taacaaaaac gatgtaaacc atcgtcagga
     8161 aggtggtaaa accggcgatc acttcggtcc gtgccgtcgt gccatgttcg cgcagtttaa
     8221 acacgcgttc cagcatcccc tggccagaag cctgggttgt gtgttgttga ctcattatct
     8281 atttccgaac aaggagggaa aatccgtcgc tatcgtatac caaaatgaga caatatggac
     8341 ggctgaagat aacttttttc attgtatttg cttatacggc aacgattgcg tcatgcgaat
     8401 tggcatgacg aaaacgttaa actaacgata aaggaaagac atgtcccaga tagaagcggt
     8461 atttttcgac tgcgacggta cgctggtcga tagcgaggtc atttgctctc gcgcctatgt
     8521 cacgatgttc agggaatttg gcattcacgt tgacctgaaa gagattttta cccgttttaa
     8581 aggcgtaaaa ctctacgaaa tcatcgatat catcagtaaa gaacagggcg tgacgatggt
     8641 ccaggccgat gccgagcacg tttaccgcgc agaagtagca cgtctgtttg ataccgaact
     8701 tgaggacatc gcaggagcga acgaattgct ggcgagcatt aacgtgccga tgtgcgtcgt
     8761 ctctaacggt ccggtcagca aaatgcagca ttcgcttggt aagctcaaca tgctgcacta
     8821 cttcccggaa aaactgttca gcggatatga cattcagcgt tggaagcccg atccggcatt
     8881 aatgttccat gcggcaaaag cgatgaatgt gaacgttgaa aactgcattc tggtcgatga
     8941 ctccagcgct ggcgctcaat cagggataga tgccggaatg gaagtgtttt acttctgcgc
     9001 tgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccg agctgtcgca
     9061 gctgcctgcg ctctggaagg cacgcggctg gaatataacg cgataatcgc ctccttactt
     9121 caacacttac acatcagtca cgctggtttg agatacagaa caccaggtgt ctgatgagta
     9181 agcgtctatt ccctatgaat aatttatgtc gttattcgtc ctgtcatcca ccctctgtat
     9241 ggttaagcct gggttatatt ttgtacaata tcgggatacc gttcgaacca atccagaata
     9301 ttacgcatcg cgtcactgat gtttttatcg tctttatatt gattctcaag tctattattt
     9361 cgaacaatag taagcttaga acgtatttta tcatcacaat cgacaatttt cgcattcaat
     9421 gcatttactt catcctgcac ttctatacta ttccaattgt cccttttgtt atcaaataaa
     9481 gtccttttat cctctaatga tttattacga cttatgatta actctttaat ttctcgcaat
     9541 aagtcatcgt aatctttaat ggaagctctt atttcctgat agttttttag taaatcctcc
     9601 cagttaattg caaatgcact ggaaaaatag taaaaatcgc gagcatactt attgagcctt
     9661 gagtcgtaat ctgtcttaaa cgattttata tgttccagtt ggtttgatag tgctttaatt
     9721 tgtttttctt caacaccttc aaaaatgggt gcaagttcct tataatcaaa tgttacattt
     9781 gtagcagatt ctttatgaga ctcctttatt tgcttttgga catattcttt tggattttca
     9841 gtaagtattg ttccatctgg cggc
//
LOCUS       DAZGXS010000149         9859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGXS010000149 DAZGXS010000000
VERSION     DAZGXS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..156)
                     /locus_tag="ACHKG5_004040"
     CDS             complement(<1..156)
                     /locus_tag="ACHKG5_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870039.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYK"
     gene            234..1328
                     /gene="ddlA"
                     /locus_tag="ACHKG5_004041"
     CDS             234..1328
                     /gene="ddlA"
                     /locus_tag="ACHKG5_004041"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0870040.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1352..1564)
                     /gene="yaiZ"
                     /locus_tag="ACHKG5_004042"
     CDS             complement(1352..1564)
                     /gene="yaiZ"
                     /locus_tag="ACHKG5_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0870041.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1824..2132
                     /gene="yaiY"
                     /locus_tag="ACHKG5_004043"
     CDS             1824..2132
                     /gene="yaiY"
                     /locus_tag="ACHKG5_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0870042.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2191..3285)
                     /gene="yaiW"
                     /locus_tag="ACHKG5_004044"
     CDS             complement(2191..3285)
                     /gene="yaiW"
                     /locus_tag="ACHKG5_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0870043.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3298..4518)
                     /gene="sbmA"
                     /locus_tag="ACHKG5_004045"
     CDS             complement(3298..4518)
                     /gene="sbmA"
                     /locus_tag="ACHKG5_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP0870044.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4870..6027
                     /gene="ampH"
                     /locus_tag="ACHKG5_004046"
     CDS             4870..6027
                     /gene="ampH"
                     /locus_tag="ACHKG5_004046"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP0870045.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6028..6651)
                     /gene="iprA"
                     /locus_tag="ACHKG5_004047"
     CDS             complement(6028..6651)
                     /gene="iprA"
                     /locus_tag="ACHKG5_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0870046.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6739..9681)
                     /gene="ehaB"
                     /locus_tag="ACHKG5_004048"
     CDS             complement(6739..9681)
                     /gene="ehaB"
                     /locus_tag="ACHKG5_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP0870047.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2541 a         2443 c         2339 g         2536 t
ORIGIN      
        1 cttataatta agagaaaaaa actcaccgca tggccattta agccaggaat aaattagctt
       61 cccgttcatc atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag
      121 tacgttgcac gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc
      181 cgttttgtcg tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa
      241 aactgcgggt aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat
      301 cggcaaaaaa cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca
      361 ttgataaaca agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg
      421 atcctgccca tattgcgttg cgcccttcgg cgaccagcct cgcccaggtg ccaggtaaac
      481 atgagcatca gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt
      541 tcccgattgt ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg
      601 ccaatttacc gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg
      661 tcaccaaacg tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc
      721 gcgctaatcg tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt
      781 ttgtaaaacc ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac
      841 aatacgcaat tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag
      901 ggatcaaagg tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca
      961 cctgtggcga gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg
     1021 aagatggcgc gaaagtggta gttccggcag ccattgcgcc agaaatcaac gataagatcc
     1081 gggcgattgc cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg
     1141 tgtttttaac cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca
     1201 ccaacatcag tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga
     1261 tcacacgttt gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca
     1321 caatgtaata ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc
     1381 gacgacgaat aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg
     1441 cataggtctg ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga
     1501 atataaggcc aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat
     1561 tcatggctgc tccgttaacg aaaaacaaca tccttgcata ttctgcaaca accgtctgtg
     1621 gtgtagacaa cattttcgca attgttaata aatttaactt aaagttgcta ttttgtgact
     1681 cgcaacgaag attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat
     1741 tgacaatcta aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa
     1801 gcagatgact tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg
     1861 gaaaatatcg caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt
     1921 tttgcttttg ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg
     1981 agcgtttaat gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg
     2041 gcaccatttt cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc
     2101 tatggctgca ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa
     2161 cgccttatct ggcatgacat tcctgtttgt tcactgtttc atacaacgcg cccgccgttc
     2221 gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag
     2281 ttgaatgcca ggcaacattt cgcgggggag tgatttaccc gttttcgctt cggcaagttg
     2341 ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctagctg
     2401 acgacgaatt tcgctgtcgc tcatccccag ttttgccgcc agtttgcgcg ttgccagttc
     2461 ggttttcccg ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac
     2521 gccgctggcc ttactgaccg cgttctgaaa cgcggcatta cggctggcgt accagccagc
     2581 gttaaaatca gcaaaacggt atatcggtgc gctataactg gcgggataat tcagtaaatg
     2641 gtaagtacca aaccacaacc cgccgcggcg actaaagact tcctgacgga ctgtaccgtc
     2701 cattttccac ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg
     2761 cccaccggtg cgcaccggat taagcgaacc aaacaacgtc tgccccattg gcaccatgct
     2821 gatcagatcg tcaaaaatcc cgctcaattg cttttctgta cgcaccgaat ctaaccgttc
     2881 gctataactt ttgccgtttg gcgatttgat ttttagcgca gtatgaacca gaaaagcagg
     2941 aatgtgcatc cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg
     3001 aacggccgga tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac
     3061 gttttccagc gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca
     3121 ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc accacgctag ccacatctat
     3181 cgctttttcg ccctttttca acggctgtgg cgcctgactg ctacaccccg ctaaaaccag
     3241 aaccgccaaa aatgaaagcg aagacagatg attgactcgc gacattactt ctccttttta
     3301 gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg
     3361 cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg
     3421 gaaagcaccg cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat
     3481 cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa
     3541 atagagaatg cgagcgatgt tgaaatacat atagtgaaaa tagaggcgga aatagttttt
     3601 ccgtacggcg ctaaacagct cgcgtaccgt tggcggcgtc gcgcgcgtgg catcgtcttc
     3661 accataaacc agctctttac ggtaggcagc ctctacacgc tggtttttaa actccagccc
     3721 cggcagtttg atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc
     3781 ggcaatcacc agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga
     3841 gagcgttacc aacaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac
     3901 acccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc
     3961 gccttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg
     4021 ccagcggaac acgtagtgac tgacaaagaa gttattcagc acactgatca ctaccgcgat
     4081 cagcgcaatc cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg
     4141 cggcgaactt agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc
     4201 aaccccaact tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga
     4261 ccagtattgc caacggtgcg ggctgtagat aaaccagaac aatgcaaaaa gtcctacgca
     4321 aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat
     4381 cgggatctgc ccggaagcgc cggtgatacg cgccacccag tcccccccac cggcttgcca
     4441 gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg
     4501 gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg
     4561 tattaaccgc atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgactcata
     4621 gcaaggcgct tagttcgcca gcctggccat aagaattgtt tcgacggatt tcacaaacca
     4681 aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc
     4741 aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc
     4801 cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt caatttatgg
     4861 acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc
     4921 tccgtccatg cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt
     4981 tatgccgatc atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac
     5041 ggtaaccagc gcgtctttcg cagttatggc gaaacgcgac ctggtaataa cgttcgcccg
     5101 cagctggatt cggtcgtgcg tattgcttcc ctcaccaagc taatgaccag tgagatgctg
     5161 gtgaaattgc tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg
     5221 ccaggcgcac gcgtgccaac ctacaatggg acgcccatta cgctggtcaa tctggcaacc
     5281 cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc
     5341 tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca
     5401 ggcagtcagg cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat
     5461 gcgtccggca agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg
     5521 aaagacacca cctacactcc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc
     5581 ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg
     5641 cctggcgata tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt
     5701 aaccaggcag atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt
     5761 ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg
     5821 aaagagggtc gtccggggat tattcagaag acaggtggtg gtggcggttt cattacctat
     5881 atggcgatga tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg
     5941 acgcgcttta aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat
     6001 aaaccgttgg ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac
     6061 cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc
     6121 gacgacagaa cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga
     6181 tcgcaattct tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga
     6241 attatgccca atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca
     6301 gtaaaaagcg tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg
     6361 tagatgatat cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat
     6421 taaaccatca gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc
     6481 ttctctacgc agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc
     6541 actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa
     6601 acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc
     6661 ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt
     6721 tcttcggagt gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg
     6781 atctacgtca ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa
     6841 gctgaactga gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga
     6901 atcaccgttc acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta
     6961 cggagtcaga gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc
     7021 ataacgcatg ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc
     7081 atcaccagac tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc
     7141 cagtttgaag tcgtaaccgg ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc
     7201 gtcaacgtaa gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct
     7261 caagctacca tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt
     7321 ctggctgtct tgatccacct gaccagaacg gtcattcatg tcacctttag cgaagcctgc
     7381 agccgcaccg acgatccact tagcgttgtt accgtcaatt ttggtatcaa caccgaccat
     7441 gatgccgtta acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc
     7501 gaagtagctt acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcaaacg
     7561 agtaccaacg gtgtcttgtt ccaggttcca gatattggtg ttcgcagatg ggatgctcag
     7621 cgccatgtta gcgtagtcgg tcaactccat ctgttgcaga acaacggtgt taccgcgctg
     7681 ttcagcctga taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt
     7741 gctgtttttg tcgttgacgt agatcagctc gttgccgttg tagtcagcga tagaaccagc
     7801 accagtagcg ttgtcgatac gaactttgta gttaccggtc gcggctgcaa catggtcacc
     7861 gttgttatca acttcgttct gaccagcttc gatagaagca gtgttgtcgt tatcgccgtt
     7921 accgttaata gtcaggtgac catcagagtt cattgcgatt aagccataac cgtagtttga
     7981 cttggtagta tcgttggtac gatcgttgac cagatcggta tccattacgt aatcactgct
     8041 atggatatcg aatacaccag cgtgaatgtt gccgttagta tcagtggtac tggtcagatt
     8101 cagggtatcg gcctggaacg ggttagcatc gaactgatca acgttaatat ccaacagacc
     8161 gtcgttagtt acggtgatgg tatttgcgta cagtgcagca tcttctaaac cccagcccgc
     8221 agaaacatca tcagacattt taacggtact attgtcgatg gtcagggtat tagtcgctac
     8281 atgaccgtct tcaccgatgt tcaggttaga accgccagtc agagagacag tgtcagagat
     8341 cagacgagag tctgcaacat taacttggga accgctgtta atacttaaag tatcaatcag
     8401 agaagatttt gttgtatccc actctgaacc accgttcaaa gtcacattaa acaaaccgct
     8461 ctggtaaact tcgtttccta cgatatggcc attttcgtca tattgagtat tgatgttatc
     8521 aacaccgaca gttgatgaag gccacaggct gttagctgca atatcgagca gagttgcagt
     8581 tgcttcagta ccaacagcat atccgtcgta agaaccatca ccatcttcat caaccatatg
     8641 aacagacatt gcagcgccaa cccacttgct gccgttgttc agagtcacat ccatacggtc
     8701 tgtgccatcc caaccgttgg tatctacatc accatcagca tcgcgatagc tgttagcacc
     8761 ttgcgggaag aagttttcat cgaaattact ggagaaaaca acatcaccca tcagtgttga
     8821 gttgtctaaa gttactgtag tctgcatcgc attatcagca tacggatttg cgattgcggc
     8881 aattgcaaca tcgtctgcag tcagcgtgtt gctatagttg ctggcattac cagtatggcc
     8941 aaaccaacca gtagtacctt catcagtcca tgaaccagag gtcacagtag aatctttaac
     9001 agtaatagtg ttattaaaca cttcgctgct gtttacgcca gtgctgactg tgttgtcgtc
     9061 aatatcttcc cactcataac cctgagtcag cgtaatacct gctacatgag agttattagt
     9121 gatggacaga tcaacttcct gatccagtgt aatagcagta cccatatcgt aaacatctac
     9181 aacatgagtg tcagcggcat tattataggt accgttgtaa gtataatgct cgtagttgtc
     9241 atcgatcgtt gaattatcaa cgctcagtgt cagacgatca taaacataac cagtagcacg
     9301 gtcatcagca caatcagtag tcatgcactc agaggtaatc ataccgtgaa tagtgctgtt
     9361 tttgattgtc agactattgg cgttggtatt ggtagaaata ccgtcgtcca gatagtaggt
     9421 agaaatcacg ccgttaacgg tagcgttatt aatgaccggg tagatatcgc cgttgtaata
     9481 gctatcagcg gcatagttgt tccagcctac ataaccatca tagtaaacgt catcagcata
     9541 ggtggcgtcg ttgtagtgat ggaaagtatt gtaagtttga ccagaaatat cgttggtcgc
     9601 tgcattagcc tgagaggtga tagccagagt gcaagccaat gctaattgtg atactacaag
     9661 tttctttttc caggagtgca tttgtcatcc ctcctcaggg acgtaataag ttgatccatc
     9721 aattatcaat gcatagaaaa catcttatgt ttcgcgttga cgattatgcg gcgttataga
     9781 ggaatggttc aattattctt tcccctaagt ccgattgcgg acatatataa aaatatctat
     9841 acgcacaaag aataagttg
//
LOCUS       DAZGXS010000150         9812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGXS010000150 DAZGXS010000000
VERSION     DAZGXS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            178..687
                     /locus_tag="ACHKG5_004049"
     CDS             178..687
                     /locus_tag="ACHKG5_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0870048.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            659..2588
                     /locus_tag="ACHKG5_004050"
                     /pseudo
     CDS             659..2588
                     /locus_tag="ACHKG5_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2572..2778
                     /locus_tag="ACHKG5_004051"
     CDS             2572..2778
                     /locus_tag="ACHKG5_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0870049.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2775..4367
                     /locus_tag="ACHKG5_004052"
     CDS             2775..4367
                     /locus_tag="ACHKG5_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870050.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4357..5862
                     /locus_tag="ACHKG5_004053"
     CDS             4357..5862
                     /locus_tag="ACHKG5_004053"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0870051.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5899..6246
                     /locus_tag="ACHKG5_004054"
     CDS             5899..6246
                     /locus_tag="ACHKG5_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0870052.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            6304..6555
                     /locus_tag="ACHKG5_004055"
                     /pseudo
     CDS             6304..6555
                     /locus_tag="ACHKG5_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6552..7292
                     /locus_tag="ACHKG5_004056"
     CDS             6552..7292
                     /locus_tag="ACHKG5_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870053.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            7306..7737
                     /locus_tag="ACHKG5_004057"
     CDS             7306..7737
                     /locus_tag="ACHKG5_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0870054.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7764..8177
                     /locus_tag="ACHKG5_004058"
     CDS             7764..8177
                     /locus_tag="ACHKG5_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0870055.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            8158..>9812
                     /locus_tag="ACHKG5_004059"
     CDS             8158..>9812
                     /locus_tag="ACHKG5_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0870056.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELG"
BASE COUNT         2294 a         2369 c         3078 g         2071 t
ORIGIN      
        1 ttttcgctat ttatgaaaat tttccgggat ccatgtccgg tttctcttca agttaactat
       61 atgaaaaata taaaaacagg tcttctgtga accggacatg aacaaaaaac agacatgtaa
      121 accggacatg accggttttg ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg
      181 gctacgcaga ctgaagttgc caggcattta agtctgaccg atcgccagct tcgcagattg
      241 cagaaattgc cgggtgcccc gatatcgaat aagcgagggc aactggatct ggatgcctgg
      301 cgcgattttt acatatcgta tctgaggaga agtaaaaacg atgtgcctga tggcgatagc
      361 gaagacgact atgaggagaa attgcttatt gccagatggg aactgacagc agaacaggct
      421 gttacacagc agttaaaaaa tgaggtgtca aaaggaaaac tgattgacac cgggttctgt
      481 atttttgccc tcagtaagct ggcaatggcg ttatccagta cgcttgattc catcccttta
      541 tccatgcagc gacagtttcc tgatttaaca ccgcgccatc ttgaccatct gaaaaccctt
      601 attgctaagg gggcaaatca gtgtgcgcgg gcaggggata aattaccgga tttacttgat
      661 gaatatatca gagcaacaac tgaataatat gatgagcgct gtcacaactg cattacagcc
      721 cctgataagg gcattgccgg tgacgccagt tgaatgggct gatcaaaatt attatctgcc
      781 taaagaatct tcatatggtg agggcgaatg gaaaacgctg ccattccaga tcgccatcat
      841 gaacagtatg gggaatgatc agatccgcac tgttaatctg attaaatctg cccgtgttgg
      901 ctatacaaag atgttgctgg gaggggggcg ggtattttat tgagcataaa tcccgaaaca
      961 gtctgctttt tcagcccacg gattctgccg ctgaagattt tatgaagtct cacgtggagg
     1021 cgacgattcg gaacgtgcca tgcctgaaag acctttcccc atggctgggt cgtaaacatc
     1081 gtgacaatac tctcacgctg aaacgctttt catcgggcgt cggtttctgg tgcctgggcg
     1141 gcgctgccgc caaaaactac cgtgaaaaat ccgttgacgt ggtctgctat gacgaacttt
     1201 cctcgttcga gccggatgtc gaaaaagagg gctcgccaac cctgctgggg gataagcgta
     1261 ttgaggggtc ggtgtggcca aaatccattc gcggctcgac gcctaaaatc aaaggcacct
     1321 gccagatcga aaaagccgct aacgagtcgg cgcattttat gcgtttttat gtgccctgcc
     1381 cgcactgtgg ggaggcgcag tatctgaaat ttggcgatga gtccacgcct tttgggctta
     1441 aatgggagaa ggacagccct gaaagtgttt tctacctctg tgaacatcat ggctgcgtga
     1501 tccatcagtc tgaactggac cagagcaacg ggcggtggat ctgtgaaaac acgggcatgt
     1561 ggacccgtga cggtctgacg tttttcagcg cccggggtga tgaaattccg ccgccgcgct
     1621 ccatcatgtt ccatatctgg acggcgtaca gtccgttcac cacctgggta cagattgtct
     1681 atgactggct ggatgcactg aaagatccca acggcctgaa aacctttgtg aacaccacgc
     1741 tgggcgagac ctgggaagag gccgtgggcg aaaaactcga tcaccaggta ctgatggata
     1801 aggtggtgcg ttacacggcg gcggtgcctg cccgggtggt ttatctgacg gcgggcattg
     1861 actcgcagcg aaaccgtttt gagatgtatg tctggggatg ggctccggga gaggaagcct
     1921 ttctggtgga taaaatcatc attatggggc gtcctgatga ggaagagacg ctgttacgtg
     1981 tggatgcggc gatcaacaaa aaataccgcc atgcggatgg caccgaaatg actatttccc
     2041 gtgtctgctg ggacaccggg gggatcgatg gtgaaattgt ttatcagaga tcaaaaaaac
     2101 acggtgtttt ccgggtgctg ccggtaaaag gcgcatctgt ctatggcaag ccggtgatca
     2161 ccatgccaaa aacccgcaat cagcggggcg tgtatctgtg tgaagtggga acggacaccg
     2221 caaaagaaat tctctatgcc cgtatgaaag ccgatccctc gcctgcggat gaagccacgt
     2281 cgtatgccat ccgttttcct gatgatccgg agattttttc gcagacagag gcgcagcaac
     2341 tggtggcgga agagctggtg gagaagtggg aaaaaggaaa gatgcgtctg ctgtgggata
     2401 acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg gcattacgtg
     2461 tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg gaagaagaga
     2521 cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg aatggttaca
     2581 gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg taaacgggtg
     2641 gtgtctgtcc agaaagatgg tcgcagaatt gaatatacgg cggcttctct ggatgagctt
     2701 aaccgggcga tcaatgatgc ggagtcggta ctggggacaa cccggcgtcg ccgtcgtccg
     2761 ctgggagtga ggttatgaaa cgaacgcctg tcctgattga tgtgaacggc gttccgcttc
     2821 gtgagagtct cagctacaac gggggcggtg caggatttgg cgggcaaatg gcggagtggt
     2881 tgccaccggc gcagagtgcc gatgcggccc tgctgcccgc gttgcgtctg gggaatgccc
     2941 gggcagatga tctggtgcgc aataacggaa tagcggccaa tgcggtggca ctgcataagg
     3001 atcacattgt cgggcatatg tttcttatca gctaccgtcc gaactggcgc tggctgggga
     3061 tgcgggagac cgcagcaaaa agctttgtcg atgaggtgga ggcggcctgg tcggaatacg
     3121 ccgaagggat gtctggcgag atcgacgtgg aaggaaaacg cacgttcacg gaatttatcc
     3181 gtgaaggtgt gggcgttcat gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata
     3241 cggaaaccac gcagttattc cgtacgcgtt ttaaagccgt gagtccgaaa cgggtggaca
     3301 cgccaggaca cggtatgggg aaccgttttc tgcgggccgg ggtggaggtc gatcgatatg
     3361 gccgtgccgt tgcgtaccat atctgtgagg atgattttcc tcgctccggg agtggacgat
     3421 gggaacggat cccgcgtgaa cttcccaccg ggcgtccggc catgctgcat attttcgagc
     3481 cggtggagga cgggcagacc cgtggggcca accagtttta cagcgtcatg gaacggctga
     3541 agatgctcga ttccctgcag gcaacacagc ttcagtcggc cattgtgaaa gccatgtatg
     3601 cagcgacgat tgaaagtgac cttgataccg aaaaggcctt tgaatatatc gccggtgcgc
     3661 cgcaggggca gaaggataat ccgcttatta atattctgga gaagttctcc agctggtatg
     3721 acacgaataa cgtgacgctg ggtggtgtca aaattccgca ccttttcccc ggggatgatc
     3781 tgaaactaca gactgcgcag gattcagaca atggattttc ggcgcttgaa caggcgctgc
     3841 tgcggtatat cgccgccggt cttggcgttt cctacgaaca gttgtcccgt gattactcga
     3901 aggtcagtta ttcaagtgcc agggcctctg ccaatgagtc gtggcgctat tttatggggc
     3961 ggcgaaaatt tattgcggcc cggctggcca cgcagatgtt ttcctactgg ctggaagagg
     4021 cacttcttcg ggggattatc cgtccgccac gggcgcgttt tgatttttat caggcgcgat
     4081 cagcctggtc acgggcagag tggattggtg ccggaagaat ggccattgac gggctcaagg
     4141 aggttcagga atcggtgatg cgcattgagg ccggactgag cacgtatgag aaagagccgg
     4201 cgctgatggg cgaggattat caggacattt tccgccagca ggtcagggaa tctgcagagc
     4261 ggcaaaaagc cggactctca cgtccggtgt ggatagcgca ggcgtatcag cagcagatag
     4321 cggagagtcg caggccggaa gaggagacaa caccccgtga gacgtaatct ttcacacatt
     4381 attgccgcag cattcaatga accgctgctt ctggagcccg cctatgcgcg ggttttcttt
     4441 tgcgcgctcg ggcgcgagat gggggcagca agtctttcgg taccacagca gcaggtacag
     4501 cttgatgctc ccggaatgct ggctgaaacg gacgagtaca tggccggagg taaacgaccg
     4561 gcccgtgttt accgggtggt gaacggtatt gctgtactgc cggtgaccgg cacgctggtg
     4621 caccggctgg ggggtatgcg gccattttcc ggaatgacag gctatgacgg cattgtcgcc
     4681 tgtcttcagc aggcaatggc ggatagccag gtgcggggcg tactgctgga cattgacagt
     4741 ccgggcgggc aggccgccgg cgcgtttgac tgcgctgaca tgatttaccg cctccgtcag
     4801 cagaagccgg tctgggcact gtgcaatgac acggcctgtt ctgcagccat gctgctggcg
     4861 tcggcctgct cccgacggct ggttacccag acatcccgta tcggctccat tggcgtgatg
     4921 atgagccatg tcagctatgc cggtcatctg gcgcaggccg gtgtggatat cacgctgatt
     4981 tactcagggg cgcacaaggt ggatggcaat cagtttgaag ccttaccggc agaggttcgc
     5041 cagaacatgc agcagcgcat tgatgcggcg cgccggatgt ttgccgaaaa agtggccatg
     5101 tttaccggtc tgtctgttga tgccgtcacg ggaacagagg ccgccgtttt tgaaggtcag
     5161 tccggcattg atgccgggct ggcggatgaa ttagtcaatg cgtcggatgc catcagtgtg
     5221 atggccacgg cgctgaacag taatgtcaga ggaggcacta tgccgcaatt aactgcaacg
     5281 gaagccgccg cgcaggagaa ccagcgagtg atggggatcc tgacatgcca ggaagcgaaa
     5341 ggacgtgaac agcttgccac gatgctggca ggacaacagg gcatgagcgt tgaacaggcc
     5401 cgggcgattc tggccgcggc ggcaccgcag cagccggtgg catccacgca gagtgaagcc
     5461 gatcgcatta tggcgtgtga agaagcgaac ggtcgtgaac aactggcggc aacgctggcg
     5521 gcgatgccgg agatgacggt ggaaaaagcc cgcccgatcc tggctgcttc accgcaggcg
     5581 gatgccggac cctcactccg tgatcagatc atggcactgg atgaggcaaa aggggctgag
     5641 gcgcaggctg aacagctggc tgcctgcccg ggaatgactg tggagagcgc ccgggctgtg
     5701 ctggctgcgg gatcaggtaa ggcagaaccg gtctctgcat ccacaaccgc cctgtttgaa
     5761 cgcatcatgg cgaaccattc accggctgcg gtacagggtg gcgtgccaca gacgtcagca
     5821 gacggtgatg cggacgtgaa aatgctcatg gccatgccat gaagtcagtg ctgaccatca
     5881 acaggaggtt tttacaatat ggtaacgaaa aacatcactg aacagcgtgc ggaagtacgt
     5941 atttttgccg gtaatgatcc ggctcatacc gccacaggca gcagcgggat ttcctcggca
     6001 acaccggcac tgacgcccct gatgctggat gaagccagcg ggaaactggt ggtctgggac
     6061 ggacagaaag ccggtagtgc agttggcata ctggtactgc cgcttgaagg cacagagacg
     6121 gtactgacct attacaagtc ggggaccttt gcgacggagg caatccgctg gcctgaaagt
     6181 gtggatgaac acaaaaaggc aaatgccttt gccggcagtg ccctgagtca cgcggcgctg
     6241 ccgtaacacg ttatcaggcc accgcggtgg cctgactgat ttctgaatga aaggaactga
     6301 tttatgggat tgtttacgac ccgccagtta ctcggttata ccgaacaaaa agtgaaattt
     6361 cgtgcgctgt ttctggagct gtttttccgc cgtacggtga atttccatac cgaagaggtg
     6421 atgctggaca aaattaccgg aaaaacgccg gtggcggcct atgtctcccc ggttgttgaa
     6481 ggaaaagtgc tgcgtcatcg cggtggtgaa acccgcgtgt tacgtccggg ctacgtcaag
     6541 ccgaaacacg aattcccctg gagccggtaa aaggagccgg taccaccctg tgggtttata
     6601 acggtcaggg tgacgcctat gcaaacccgt tgtcagacga tgactggcag cgactggcta
     6661 aggtgaagga tctgacgccg ggcgagatga cggcagaatc ctacgatgat aactacctgg
     6721 atgatgaaga cgcggactgg accgcgaccg ggcaggggca gaaatctgca ggtgatacca
     6781 gttttacgct ggcctggaaa ccgggagagg aaggccagaa agggcttata ggctggtttg
     6841 aaagcggcga tgtccgggcc tataaaatcc gttttccgaa tggcacggtg gatgtgtttc
     6901 gtggctgggt cagcagtatc ggtaaggccg tgacggcgaa agaagtgatc acccgcacgg
     6961 tgaaagtcac taacgtgggt aaaccttctg tagcggaaga acgcagcaaa attacgccgg
     7021 tcagtgcgat taaggtgacg ccgacatccg gtacggtggc aaaagggaaa acaaccaccc
     7081 tgacggtttc ttttgagccg gaaagtgcaa cagacaagac gttcagagcg gtttccgccg
     7141 atccgtcgaa agccaccatt agtgtgaaag atatgacaat tacggtaaac ggcgtggcga
     7201 caggtaaggt gcagatccct gtggtgagcg gaaatggtca gttcgccgca gtggctgaag
     7261 tcaccgttac tgaagcgggc gctgcagggt aaacggaggt catacatgtt tctgaaaaca
     7321 gaacaatttg aatataacgg tgtgtctgtc acgctttccg aattgtctgc gctgcagcgt
     7381 atcgagcatc ttgccctcct gaaacggcgt gcagaacagg cagaatccag cggcaacctg
     7441 caggtaagcg tggaagatct cgtcagaacc ggcgcgtttc tggtggcgat gtccctgtgg
     7501 cataaccatc cacagaaaac gcagtcaccg tcaatgaatg aggccgtgat gaagatagag
     7561 caggaagtgc tcaccacctg gcctgccgat gccattgccc gggcggaaga cgtggtgttg
     7621 tgcctgtccg ggatgagcgg ggctgttcgt ccggatactg atattactga agtggcgaaa
     7681 aataacacgc tgactgatga tgatttttct gcgggaaagt cttcgacggc gagctgaact
     7741 ttgccctcag actggcgcgt gagatgggga gacccgactg gcgcgccatg cttgccggga
     7801 tgacatccac cgaatatgcc gactggcacc gtttttaccg cacgcattat tttcaggata
     7861 cccagctgga tatgcatttt tccgggctga cgtacgctgt actcagcctg tttttttgcg
     7921 atccggatat gcatccctct gatttcagtc tgcttgtccc ccggcatgag gaagagcagg
     7981 tggagaggcc ggatgaggac aaaatgctga tgcagaaagc ggcaggactt gccggaggcg
     8041 tccggttcgg tggggacgga gggcgcgata ttttatcgtc tgcggatgtg gcggatgtca
     8101 tggtggatga tgccgcatta atgatggctt cagcggggat tccgggaggt gtgagatatg
     8161 tcccagccgg ttggtgatct tgttattgac ctgagtctgg atgctgtccg tttcgatgag
     8221 cagatgagcc gggtaaggcg tcatttttca ggtctggata ccgacgtcag aaaaaccgcc
     8281 agtgctgttg aacagggcct gagccgccag gcgctggctg cacaaaaagc cgggatttcc
     8341 gtcgggcagt ataaagcggc catgcgaacc ctgcccgcac agtttacgga tatcgccacg
     8401 cagcttgccg gtggtcagaa tccctggctg atcctgctgc aacagggcgg tcaggtgaag
     8461 gactccttcg gcgggatgat ccccatgttc agggggcttg ccggtgcgat caccctgccg
     8521 atggtcgggg tcacctcgct ggcggtggcg acaggtgcgc tggtgtacgc ctggtaccag
     8581 ggagattcca cgctttcagc gtttaataaa accctggttc tttccggtaa tcagtccgga
     8641 ctgactgccg atcgcatgct gacgctctca agagccgggc aggcagcagg gctgacgttt
     8701 aaccaggcga gagagtcact ggcagccctg gtgaatgccg gtgtgcgtgg tggtgaacag
     8761 tttgatgcca tcaaccagag tgtcgcgcgt tttgcttctg catccggtgt ggaggtggac
     8821 aaggttgcag aggctttcgg aaaactgacc accgacccta cgtcggggct gattgcgatg
     8881 gtgcgccagt tccgtaacgt gacggcagag cagattgcgt atgttgcgca gctgcagcgt
     8941 tccggtgatg aggccggggc cttacaggcg gcgaacgata tcgccacgaa aggctttgat
     9001 gagcagaccc gtcgcctgaa agaaaacatg gggacactgg agacctgggc ggataaaacc
     9061 gggaaggcat tcaaatcgat gtgggatgcc attctggata tcggtcgtcc tgagtcctca
     9121 gcggatatgc tcgccagtgc acagaaggca tttgatgagg cggataaaaa atggcagtgg
     9181 taccagagcc ggagccagcg ccggggaaaa accgcctctt tccgggccaa ccttcagggc
     9241 gcatggaatg accgggaaaa tgcccgtctg gggctggcag cggccacgct gcagtcggat
     9301 atggaaaaag ccggtgaact ggccgccagg gaccgggccg aacgggacgc atcacagctg
     9361 aagtataccg gagaggcgca gaaggcgtat gagcgtctgc tgacgccgct ggagaaatat
     9421 accgcccgtc aggaagaact gaataaggcc ctgaaagacg ggaaaatcct gcgggcggat
     9481 tacaacacgc tgatggcggc ggcgaaaaag gattatgaat cgacgctgaa aaagccgaag
     9541 tcgtcaggag tcaaagtgtc agccggtgag cgtcaggaag accaggcgca tgctgccctg
     9601 ctggcgcttg aaaccgagct caggacgctg gaaaaacaca gcggtgcgaa tgagaaaatc
     9661 agccagcagc gtcgcgattt atggaaagcg gaaaatcagt atgcggtcct gaaagaggct
     9721 gccacgaaac ggcagttatc tgagcaggaa aaatccctgc tggcgcataa agacgagacg
     9781 ctggagtaca aacgccagct ggctgagctg gg
//
LOCUS       DAZGXS010000151         9726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGXS010000151 DAZGXS010000000
VERSION     DAZGXS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..772
                     /locus_tag="ACHKG5_004060"
     CDS             <1..772
                     /locus_tag="ACHKG5_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0870057.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            798..1592
                     /gene="yfaU"
                     /locus_tag="ACHKG5_004061"
     CDS             798..1592
                     /gene="yfaU"
                     /locus_tag="ACHKG5_004061"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP0870058.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1633..2559)
                     /gene="rpnE"
                     /locus_tag="ACHKG5_004062"
     CDS             complement(1633..2559)
                     /gene="rpnE"
                     /locus_tag="ACHKG5_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0870059.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2752..3942)
                     /gene="glpC"
                     /locus_tag="ACHKG5_004063"
     CDS             complement(2752..3942)
                     /gene="glpC"
                     /locus_tag="ACHKG5_004063"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0870060.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3939..5198)
                     /gene="glpB"
                     /locus_tag="ACHKG5_004064"
     CDS             complement(3939..5198)
                     /gene="glpB"
                     /locus_tag="ACHKG5_004064"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0870061.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5188..6816)
                     /gene="glpA"
                     /locus_tag="ACHKG5_004065"
     CDS             complement(5188..6816)
                     /gene="glpA"
                     /locus_tag="ACHKG5_004065"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0870062.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7089..8447
                     /gene="glpT"
                     /locus_tag="ACHKG5_004066"
     CDS             7089..8447
                     /gene="glpT"
                     /locus_tag="ACHKG5_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0870063.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8452..9528
                     /gene="glpQ"
                     /locus_tag="ACHKG5_004067"
     CDS             8452..9528
                     /gene="glpQ"
                     /locus_tag="ACHKG5_004067"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0870064.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2224 a         2404 c         2686 g         2412 t
ORIGIN      
        1 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggcgctg gggtattcac
       61 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac tagaaaaaac
      121 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
      181 tgcgctgcgt aatgggcgag tctggcaact ggcgattatc tacctgacca ttcaggtggc
      241 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
      301 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
      361 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
      421 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtac tggcgatcgt
      481 agcgctgtgt gttgcagcca tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
      541 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgcttgg
      601 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
      661 cgatgcggcg ggattactga cgctggcagc ggtggcggtc atcggttcgc tgattatttt
      721 cactctgcgt gtaaatcgca ccgttgcgca gaccgacgtg gcacatcatt aaataggtta
      781 aggaacacga tgaacgcatt attaaccatc cctttaaaga acgtttacgc aagggcgaag
      841 tgcaaattgg tctgtggtta agctcaacga ctgcctatat ggcagaaatt gccgctactt
      901 ccggttatga ctggttgctg attgacgggg agcatgcgcc aaacaccatt caggatcttt
      961 atcaccagct acaggcggta gccccctatg ccagccaacc cgtgatccgt ccggtggaag
     1021 gcagtaaacc gctgattaaa caagtcctgg atattggcgc gcaaactcta ctgatcccga
     1081 tggtcgatac tgccgatcag gcacgtcagg tggtgtctgc cacgcgctat cctccctacg
     1141 gtgaacgtgg tgttggggcc agtgtagcac gggctgcgcg ctggggacgc attgagaatt
     1201 acatggcgca agtcaacgat tcgctttgtc tgttagtgca ggtggaaagt aaaacggcac
     1261 tggataacct ggacgagatc ctcgacgtcg aagggattga tggcgtgttt attggacctg
     1321 cggatctttc tgcgtcgttg ggctacccgg ataacgccgg gcacccggaa gtgcagcgaa
     1381 ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa agcggctggt tttctggctg
     1441 tggctcctga tatggcgcag caatgcctgg cgtggggagc gaactttgtc gctgttggcg
     1501 ttgacacgat gctctacagc gatgccctgg atcaacgact ggcgatgttt aaatcaggca
     1561 aaaatgggcc acgcataaaa ggtagttatt gatatcaaag gcccatgggg atcggctgtg
     1621 ggactgtgtt aattagtggt tattcgctgc cagatcggct tcgcttagct gggtggccgc
     1681 gagcaccagg tcacgatcaa agccctgttc cagcatgcgc aaggcaattt taatagcttg
     1741 ttcatgcatg ccttcctgca aaccttctaa tttaccttcc tgccaaccaa tttgatgtcc
     1801 ttcctgccgt agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc
     1861 ggcaatctcc tgaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt
     1921 aaacagtgtt tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct
     1981 gtcgaccata ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt caagcagagc
     2041 tatacgccga tgttgcatga tctcatcgtc aggtacgatg gtaatatcca ccaacggaaa
     2101 agcttcggtg tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttgag
     2161 tgagtacggg tagggcgagg tttcgccatg ataaaacagc aacggcacca ccagcgggac
     2221 tctgtcatag cctttgtcca ggtgacgctg catggcggca gtggcatagc gcattagccg
     2281 aaaagccata ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccgtc
     2341 accgtcgctg gtttccacgg accacaaaac atccgagtag taagcgcgta aacttttttc
     2401 aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc
     2461 tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt gtgaacataa aggttttaaa
     2521 tactgcatca tgcggggagg aggttgttga ttcggtcatg gttgtccgtc acctgatatt
     2581 tatgatgacg cgacattacg gttatcccag gcctttgaca tctgccagtt tcagcctttg
     2641 cgagacgttt tcagaggaat tttcactgcg ttgcaacgca tacattgggc acggaatcgt
     2701 gtacgcaaaa atgcccggta accacatgct taccgggcat cagaaaggag tttaagccag
     2761 cgcctgggcc agtagcgtaa tcggatgttc gcagcgaaga cttgtggaca tctcaatctg
     2821 ccatttacag gtttcgcagt cggtgaccac cagatctgcg ccgctttctt ctatctggcg
     2881 gaacagtggt gcgccgatgg cttgtgaggt ggggtagttc tcttttttga aaccgtaagt
     2941 acccgcaata ccgcagcact gggaatccag caccgttaac tcaagccccg ggatgttacg
     3001 caacagctcc agggtgtaga gcgtccagcc cattttttcc atatggcacg gagtgtgata
     3061 aaccactttc agcggcagcg gtttcagcgg taacgttttg ccttcgtcca gcttgcgcca
     3121 cagccagcgg gtcgccagtt cgatatgatc gcgcaagcct ttgttgtcga cattcagcac
     3181 ttccgggtat tcgtcgcgca gggcaaatgt acaggttgag gaggtggcaa tcaccggaat
     3241 gccttttact cccacagctt cgcggatcga ctctacattc gtaattgcct gtttgcgtgc
     3301 tttatcggta aagccgttgg cgatcagcgg tacgccgcag catttttctt tgctgagcag
     3361 ttgtacaccg gtacccattg cgttgagcac tttaattaaa tctttaccta actgcggatg
     3421 gttgtagtta acgaagcagc cgtgaaagaa agcgacctgg tctttatact gcgcttgctg
     3481 agccgccacg ctgcgatacc agcgacgaaa cgtgccgaag gagtatttcg gtagcgtgcg
     3541 gcgatgatcg atttttaacg ccgcatcaag caactgccgc accggtttca gcgaggtagc
     3601 ggtgttgacg attggtgcga acggcgtgga aacgctaccc atcaggtcgg tatggcttaa
     3661 aacaaaattg cgcagcgacg ggcgcgtggt gtcatatttc gcccgtgcgc gctggatgat
     3721 atcgccaatc ttcacatcgg acgggcaggc gacttcgcaa cgtttgcagt tgatgcaata
     3781 tttcagtgcc tcgtcataca gtgcgccatc ttttaaacgc agacgctcgc catccggccc
     3841 ggcttgtttt ggccctggat aaccgggatt cacccggctc actgggcagg cggtggtgca
     3901 gacggtgcac ttaatgcagt tttcgaagct ggtgtcattc attgttggcc tcctgcgcgt
     3961 tgggcaatct gttgagcggc atgtaaagca ctgacggcac aaacaccgcc gccgcatccc
     4021 tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg taaacaggtt ttcgatggtt
     4081 tgccctgcct gcgacgggcg tagcgtctga tcagtggtta caccgaactg ctgccacggt
     4141 tgcggcgcaa aaaaatctcc cttataccat tcaccccgcg tggcggtttg tagcacatca
     4201 aggccgagaa tcggctctcg aatgccgtta cgttccgcta ccagtccgcc actaaagaaa
     4261 ctgccgctgg cgagaaccgc gaaacgtgga cgcagcggga tatcggcgtg attgcgggtc
     4321 cagatttcat tcactacgcc atttttacag gtcacttttt tcacttcatc gcccggcatc
     4381 cacacgccac cctgacgcac aaactggcgc tgtaactggt tttgcagacg aatgcccagc
     4441 acggaaggcg gcagcgttgg caaaagcatc agtgaacaag gtagtttttc attcaaccaa
     4501 cgccacagtt tgtcatcagc caggccgaag caggcgggca tcaggatcat ttcgcaggta
     4561 ttggcgacag gaataagcgc atcaagtaac agcggccagt tttcttcatt atcaaggaaa
     4621 cgggcgatat tcaccgcgcg gaactcggtg gcgttattgc gcaggacatc cagttccggc
     4681 agctctattt ctgcggtttc aacggcaagg tcgagttcac gcaacgaagc tgccgcaagg
     4741 tgcgcctgaa aatccatcag tccgctaatt cccactacac atattttctt cgcgggcagc
     4801 ggccagacgg ggacttctgg cgaacttagc caggtagagc gcagagtgcc gagcggcgta
     4861 atccgctggt gaggcagttc tacgctgccc tgcaattgcg caccgctttc agcgattaat
     4921 gcctgcgcct ggcaagcgag atcgagcacg cgttgtggcc cgagaagaga gtaaggatgt
     4981 gcaggtgcct gctgacgcaa agattccagt ccactgtgaa tgtctgtcac agcttgacca
     5041 tctggcagat ggctcagcaa atccagcgaa ccggatgaga aatgcagcgc gctttgacca
     5101 cgcgtgacaa tggcacagcg caggccgtgt ttttgcagct gcaggccaca gagtaatccg
     5161 gcgaggccgc cgcccataat gatagtatca aagcgcatct ttctgctcct tctccagacc
     5221 acacagtccc tgataaaccc agcgggtaaa ttcgctttcg cgcagtgcat ctccccaggc
     5281 gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg gaaagttgct cgatagattg
     5341 cgcggacgtc gtgacgttaa aacgttgcag cagtccagca gcgcggcagg cgcagagttc
     5401 gccctggcag gtacccatcc ccacacgggt acgacggcgt aaatccagca ggctattaac
     5461 gtttaaattt tctaccgcgt actgcacttc acccgcagtt accgcttcgc actcacatac
     5521 caggctacgg tgcagacggc cttcgctcag ccaggcaggc gtgcgatcgc catgacgata
     5581 aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact ttacgcaagg taacttcagc
     5641 gggttcctgt gaacccggaa gtgccagatc ggcggtcgta caggggcgcg tgttgcccag
     5701 tttgcggcat accgcgtcgg tagcccattc agccatcagc cgataggtca tcagtttgcc
     5761 accggtgatg gtgataaatc cgtccagacc atcgcgttca gcatggtcga gcagcacgat
     5821 gccacggctg acgttacgtc cgctcgggtc gtcatcgctg gcaaccagcg ggcgcacgcc
     5881 agaataggcc cgcaaaatgc gcgttttcgc catcacgggg gccagttttt ccccttcacg
     5941 cagcagaata tcaacctctt ctgccgtcac tcgattatcg tcaatctcgt tgtaatcaat
     6001 acgtaaagag gtggtgccaa tcagcgaaat ggtatcgcca ggcaccagaa tatcggcgtc
     6061 ggaaggttta cggcagcggt tgatcacatg ctgattaatg cggtgatcca tgatcagcag
     6121 cgatcctttc gccgggaaca tgcgaatgcg cagatcggca tattcggcaa tgtgttgccc
     6181 ccagatccca gcggcattaa ccacgacagg tgcatgaagg gcctgagttt cgccggtgag
     6241 atggttacgt acacgaacac cgcacaccgt cgcgccttca cgaatcagcc ccgtgacttc
     6301 atgagcggta aggataacag caccgtgttc tttggcatcc agcatgtttg ctgcggtcag
     6361 acgaaatgga tcaacggtgc catccggaac tttcaccgcg ccaatcagtg ccgggttaac
     6421 ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct tctgcgctga tccctgcttc
     6481 ttcgcaggcg cgaataaaag tggcctggaa ggagaggtca tcttccggca gggtgataaa
     6541 caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt ttcaggatct ggttttcact
     6601 aatgcattcg cgggccgatt ccgcatcggt taccgcatag cgcgcaccgc tgtgcagcag
     6661 gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg cgctcaacca aaatcacgcg
     6721 cagcccgcgc agggcacagt cgcgggcaat tccggctccc gttgcgccgc cgccaatgat
     6781 aatcacgtca cttgattgcg agtcgcgagt tttcattgtt atccctctga agttcgtttt
     6841 ttaccattta gccatagtaa aaacatgagt tgtttgattt cgcgcatatt cgctcataat
     6901 tcgaaagtga gacgtgattt catgcgtcat tttgaacatt ttgtaaatct tatttaataa
     6961 tgtgtgcggc aattcacatt taatttatga atgttttctt aacatcgcgg caactcaaga
     7021 aacggcaggt tctctcactg aatcaggctg ttaatcataa ataagaccac gggccacgga
     7081 ggctttcaat gttgagtatt tttaaaccag cgccacacaa agcgcgctta cctgccgcgg
     7141 agatcgatcc gacttatcgt cgattgcgct ggcaaatttt cctggggata ttctttggct
     7201 atgctgctta ctatttggtt cgtaagaact ttgcgctcgc tatgccttac ctggtggagc
     7261 agggattctc acgcggtgat ttaggttttg cgctttctgg gatctcgatc gcctatggat
     7321 tttcgaaatt catcatgggt tcggtatcgg atcgctcgaa tccgcgcgtt ttcctgcccg
     7381 caggtttgat tctggcggcg gcagtgatgt tgtttatggg ctttgtgcca tgggcgacgt
     7441 cgagcattgc ggtgatgttt gtactgttgt tcctctgcgg ttggttccag gggatggggt
     7501 ggccgccatg cggtcgtact atggtgcact ggtggtcgca gaaagaacgt ggcggcattg
     7561 tgtcagtgtg gaactgtgcg cacaacgtcg gtggtggtat tccgccgctg ctgttcctgc
     7621 tggggatggc ctggttcaat gactggcacg cggcgctcta tatgcccgct ttctgcgcca
     7681 ttctggtggc attattcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcttgc
     7741 cgccgatcga agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg
     7801 agctgaccgc gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ttgtggtata
     7861 tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga
     7921 cttatctgaa agaggttaag catttcgcgc tggataaatc ctcctgggcc tacttcttct
     7981 atgagtatgc gggtattccg ggcaccctgc tgtgcggctg gatgtcggat aaagtcttcc
     8041 gtggcaaccg tggggcaacc ggcgttttct ttatgacact ggtgaccatc gcgactatcg
     8101 tttactggat gaacccggca ggtaacccaa ccgtcgatat gatttgtatg attgttatcg
     8161 gcttcctgat ctacggccct gtgatgctga tcggtctgca tgcgctggaa ctggcaccga
     8221 aaaaagcggc aggaacggca gcgggcttta ccggtctgtt tggttacctg ggcggttcgg
     8281 tggcggcgag cgcgattgtt ggctacaccg tggacttctt cggctgggat ggcggcttta
     8341 tggtaatgat tggcggcagc attctagcgg ttatcttgtt gattgttgtg atgattggcg
     8401 aaaaacgtcg ccatcaggaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg
     8461 acgctgaaaa accttagcat ggcgatcatg atgagcacta tagtcatggg aagcagtgca
     8521 atggcggcgg acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg
     8581 ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa
     8641 caggatttgg tgatgaccaa agacgaccat ctggttgttc tgcatgacca ttacctcgat
     8701 cgtgttactg atgttgccga tcgtttcccg gatcgggcgc gcaaagacgg tcgttactac
     8761 gcgatagatt tcacgctgga tgaaattaag tcgttgaaat ttaccgaagg tttcgatatt
     8821 gaaaacggta aaaaagtgca gacttatcca gggcgtttcc caatgggtaa gtccgacttc
     8881 cgggtgcaca cctttgaaga agagattgaa tttgttcagg ggttaaatca ctctaccggg
     8941 aaaaatatcg gtatctatcc agaaatcaaa gcgccgtggt tccatcatca ggaagggaag
     9001 gatattgcgg caaaaacgct ggaagtgctg aagaaatatg gttacaccgg taaagacgat
     9061 aaagtttatt tgcaatgttt tgatgctgat gagctgaagc gtattaagaa tgagctggaa
     9121 cccaaaatgg gcatggatct caatctggta cagctgattg cctataccga ctggaatgaa
     9181 acgcagcaga aacagccgga cggaagctgg gttaattaca actacgactg gatgtttaag
     9241 ccgggtgcca tgaaacaggt ggcggaatat gcagatggta ttggtccgga ttaccatatg
     9301 ttgattgagg agacatcgca gccgggtaat atcaaactca ctggcatggt gcaagatgct
     9361 cagcagaaca agctggtagt gcatccttat accgtgcggt cagataaact gcctgaatac
     9421 acaactgatg tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggttg
     9481 tttactgatt tccctgataa agcggttaaa ttccttaata aagagtaatg ctgctactgc
     9541 aggatggcgt tcatgcgcca tcctctgcta aagcgaaata atgctactac cggttattct
     9601 tttcattaac cggtgcgtcc tgtaaaaaag atgccaacat aattttcgta aaactcattc
     9661 gttaaaaaag ctaacactgg gcattgttat tctttaaaca gtatgaatag gtctcaataa
     9721 taaatt
//
LOCUS       DAZGXS010000152         9720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGXS010000152 DAZGXS010000000
VERSION     DAZGXS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHKG5_004068"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHKG5_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0870065.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHKG5_004069"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHKG5_004069"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0870066.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHKG5_004070"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHKG5_004070"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0870067.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="ACHKG5_004071"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="ACHKG5_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0870068.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="ACHKG5_004072"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="ACHKG5_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0870069.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="ACHKG5_004073"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="ACHKG5_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0870070.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="ACHKG5_004074"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="ACHKG5_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0870071.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHKG5_004075"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHKG5_004075"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0870072.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHKG5_004076"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHKG5_004076"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0870073.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2462 a         2596 c         2291 g         2371 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
//
LOCUS       DAZGXS010000153         9587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGXS010000153 DAZGXS010000000
VERSION     DAZGXS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(110..322)
                     /gene="cspA"
                     /locus_tag="ACHKG5_004077"
     CDS             complement(110..322)
                     /gene="cspA"
                     /locus_tag="ACHKG5_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0870074.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(603..893)
                     /locus_tag="ACHKG5_004078"
     CDS             complement(603..893)
                     /locus_tag="ACHKG5_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0870075.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1327..2037
                     /locus_tag="ACHKG5_004079"
     CDS             1327..2037
                     /locus_tag="ACHKG5_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0870076.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2087..3061)
                     /gene="ghrB"
                     /locus_tag="ACHKG5_004080"
     CDS             complement(2087..3061)
                     /gene="ghrB"
                     /locus_tag="ACHKG5_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0870077.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3165..3824)
                     /locus_tag="ACHKG5_004081"
     CDS             complement(3165..3824)
                     /locus_tag="ACHKG5_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0870078.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3977..6310
                     /gene="bisC"
                     /locus_tag="ACHKG5_004082"
     CDS             3977..6310
                     /gene="bisC"
                     /locus_tag="ACHKG5_004082"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0870079.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6279..6719)
                     /locus_tag="ACHKG5_004083"
     CDS             complement(6279..6719)
                     /locus_tag="ACHKG5_004083"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0870080.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6716..7279)
                     /gene="tag"
                     /locus_tag="ACHKG5_004084"
     CDS             complement(6716..7279)
                     /gene="tag"
                     /locus_tag="ACHKG5_004084"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0870081.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7437..8135
                     /locus_tag="ACHKG5_004085"
     CDS             7437..8135
                     /locus_tag="ACHKG5_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0870082.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8364..9566
                     /locus_tag="ACHKG5_004086"
     CDS             8364..9566
                     /locus_tag="ACHKG5_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0870083.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2266 a         2355 c         2482 g         2484 t
ORIGIN      
        1 aaataatact agattttatt acgcgatcga ttatttattt cctgaaaaac aaataaaaaa
       61 atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat tacaggctgg
      121 ttacgttgcc agctgccggg cctttagcgc cgctttcgat ggtgaaggac actttctgac
      181 cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg aacacgtctt
      241 tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac cattttacga
      301 taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg cctttcggcg
      361 atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga ggggtatcaa
      421 cgataactct tgaagggact tgccttacta cactgcttta atggtctgta cgtcaaaccg
      481 ttgatgtgca ttaagccacg cattggcggg tgatgcaaca attatttttc atatttatga
      541 ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa gtggataaaa
      601 gtctattcca tcaactgctt acttaatgcc gggttggctt gaatcaaacg catcaatttt
      661 agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat ggcgactgat
      721 acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttcg taactgctca
      781 atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc atctttaaaa
      841 acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc cattgaaaac
      901 tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa tagtcgggaa
      961 aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat tcagccagtc
     1021 atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc tctaaccact
     1081 cctggtttgt aaagatttcg aaaatctaac cttctgcgaa ggcgtaaatc tctggctaca
     1141 aaaaaggcaa cggcaatatc attttttaac aatgaaacaa caaattaacc ttgtttcatt
     1201 ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc ctgtcctgga
     1261 ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg tgaaaggagt
     1321 aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc ggcgttatta
     1381 atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa agcgtttatc
     1441 gacttcctgc agaatacagt gatgcgtagc ggtgaacgtc taccaactct gactgccgat
     1501 cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg ttattctcag
     1561 caggtgaatc aggcgatgga ttccggtctg cgcccggttg tagacagcgt taacgcaatt
     1621 cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa tggttcgctg
     1681 ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc gcactctgcg
     1741 ctaaaacaga gtgatgacct gaaaccggtc tttgatcagg cattcaccaa agtggtaacg
     1801 acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac gcaacaactg
     1861 gtgatggttg gagactacat tgcccaacaa ggtactcagg tgagctttgt agcaaatggc
     1921 atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc gccattaccg
     1981 gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac ccaataaaga
     2041 gtaaaagccc gagcgattat cgggctttac tccacgcagt cgcggcttag tccgcgacgt
     2101 gcggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa ttatccacgg
     2161 cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc ggtactgcga
     2221 cgacgttggc cattgagagc aacggcgaat ctacggaaag tggctcttgt tcgaagacat
     2281 ccagcccggc ggcgtgaatt tcccctttct gcaatgctgc gatcagtgca ttttcgtcaa
     2341 ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg gcgaattgtt
     2401 ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa acgaaatctg
     2461 actcttgtaa cagtgtatcc aaatcgcagt agcgggcgtt gaagcgttct tctgcttctt
     2521 tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc gcacgttgtg
     2581 ccagcgccat accgatccgt cccatcccga caatgcccag tgttttatgg tgaacgtcag
     2641 tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc cgttctgcta
     2701 cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg gcgacggttt
     2761 ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc gcatcgacat
     2821 caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc attttttcca
     2881 gcaatgcggc atcaacattc tcgtttgaac ccagtaaacc ttcagcttcg gcaaaaattg
     2941 ctgcattttg ttcgacggtt tgtgggctga ggtttgccac ctggtgaacg gtgaaatgct
     3001 cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg gacggcttca
     3061 tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat gaaggaaaac
     3121 tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag cgggcttaag
     3181 gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct ggcgattggg
     3241 ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg ggtgatcaac
     3301 gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcagatc gtgaccaccc
     3361 gtgctgtcag tataaccaat cacgttaacc gccgttttcg gatactcttt cagcaccatt
     3421 gccacgccgg tcagggtgtt agcgcccgcc ggtttcaggg ttgcgctgct gctgtcgaag
     3481 gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac gctaacacca
     3541 gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata acccacgccg
     3601 ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg atctttcttc
     3661 gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc gccgatagca
     3721 gatttacctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga taccgccaga
     3781 gcgccactca ctacggcggc aataagataa acacgtttct tcattgttaa tccttaataa
     3841 cctttttatt ctttgccacg ggttccgtgg cgggagatta tgccgcgtga acatgaagat
     3901 tattcctggg aatactcgga aatttgtaag taatatttaa ctgctcaata catctaacct
     3961 ttcaggagtc ttcggtttga ccaactcatc ctcacgatat tccgttctga ctgccgccca
     4021 ttgggggccc atgctggttg aaaccgacgg cgaaaccgtg tttagctcgc gtggcgcgtt
     4081 agccacagga atggaaaact ccttgcagag cgcggttcgt gaccaggttc acagcaatac
     4141 gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg tcaccggaaa acccgcaagg
     4201 cattcgtggg caggatgaat ttgttcgcgt gagttgggat gaggcgctgg atcttattca
     4261 ccaacaacat aaacgcattc gtgaggctta tggtccggca tcgatttttg ctggttccta
     4321 cggctggcgt tcaaacggcg tgctgcataa ggcctcgaca ttattacaac gctatatggc
     4381 gctggcaggc ggttataccg ggcatctggg ggattattcg accggcgcgg cacaggcgat
     4441 catgccgtat gtcgtgggtg gtagtgaagt ttatcaacag cagaccagtt ggccgctggt
     4501 gctggaacat agcgatgtcg tggtgctgtg gagtgctaac ccactcaata cgctgaaaat
     4561 tgcgtggaat gcatccgatg agcaggggct ttcttacttt tctgcactgc gtgacagcgg
     4621 gaaaaagctg atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa
     4681 aatggagtgg gtggcaccgc acatgggcac tgatgttgcg ctgatgctgg ggatcgccca
     4741 tacgctggtg gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccaccggtta
     4801 tgccgtcttc gcctcttatt tgctgggcga gagtgacgga atagcgaaaa ccgccgaatg
     4861 ggcagcagag atttgtggtg ttaacgcaga gaaaatccgc gagctggcgg ctattttcca
     4921 ccaaaatacc accatgctga tggctggctg gggaatgcag cgccaacagt ttggtgagca
     4981 aaaacactgg atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg
     5041 cggttttggt ctttcttacc attttgccaa tggtggtaac cccacgcggc gcgctgcggt
     5101 actctcttcc atgcagggca gcttgccggg tggctgcgat gcggtagata aaatccctgt
     5161 cgcccgcatt gttgaagcac tggaaaaccc cggtggcgca tatcaacaca acggtatgga
     5221 ccgacatttc ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca
     5281 tcaggatacc aatcgcctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga
     5341 atgcttctgg acggcggcgg caaaacacgc ggatatcgtt ctgcctgcga ctacctcttt
     5401 tgagcgtaat gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa
     5461 gcaagtggtg ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttaag
     5521 tgaacgctgg gagaagggcg gttatgcacg ttttacggaa ggaaaaagtg agctgcaatg
     5581 gctggaaacg ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc
     5641 gccatttact gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag
     5701 cgagcggttt attcgcttcg ctgatttttg ccgcgatccg caggcgcatc cattaaaaac
     5761 cgccagcggt aagattgaaa tcttctcgca gcgtattgcc gattacgctt atccggattg
     5821 cccggggcat ccaatgtggc tggtgccgga cgaatggcag ggcaatgccg aaccggaaca
     5881 gttacaggta ctttctgctc atccggcgca tcgtctgcac agccagctga attacagttc
     5941 tctgcgcgaa ttgtacgcgg tggcaaaccg tgagcctgtc accattcatc ctgacgatgc
     6001 ccaggcgcgc ggcatacaag atggcgatac tgttcggctg tggaacgcac gcgggcaaat
     6061 tcttgccgga gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg
     6121 ggcatggccg gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt
     6181 gctgaccaaa gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacggcgct
     6241 ggcatggcta gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc
     6301 cagctcataa tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga
     6361 aaaccctgcg cctggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc
     6421 agatggggat agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg
     6481 gccttcggtg cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg
     6541 agaagcttac cgtcttcttc ccagacccag tttttcgcat tggcaagata cgcatcccgc
     6601 accagcggaa tgcactcgcg ccagtaattc acttttataa agggatgccc ccaggttgta
     6661 ctttccagcc acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg
     6721 gtttatttcc cggatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct
     6781 gcataaagga gtaacagatt gtggtaccga caaacttaaa accacgtttt ttcagtgcct
     6841 tagatagggc gtcggaggcg ggcgtagatg tggggatttc gctcaacgtt gtggcttgtg
     6901 tcacctgtgg ctgatgattt acaaacgacc agacaaagtc ggcaaacggt tcgccgttct
     6961 gttccatttg caggtacgcc cgcgcattac caataattgc ctgaattttc ccgcgatggc
     7021 ggataatccc ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gcggcaacct
     7081 tcaccggatc gaactgatga aagcaggcgc gatagttttc gcgttttttg aggacggtga
     7141 tccacgataa cccagcctgc tgcccttcca ggcagatcat ttcgaacagt tttttcctgt
     7201 cagtttcagg cacgccccac tcattatcat ggtaggcaat ataaagcggg tcctgactca
     7261 cccagccgca acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg
     7321 cttttcttga cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat
     7381 ccttcataca atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga
     7441 tcataaaaaa aagcggtggt cgctggcagc taagcctgct ggcgagtgtg gtaatcagtg
     7501 ccttttttct caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg
     7561 ggcattccac agagcgttac acctgggata gtgatcatca acctgattac aacgatattt
     7621 tgtcgcaacg tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag
     7681 aaacccctgt ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac
     7741 aggttacaac tggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata
     7801 acgagtttgg cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta
     7861 ccttaggctg gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct
     7921 ataaccagca atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg
     7981 cgacaaacca gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc
     8041 aaaatattgc cgcctatgcc gcgctatctc aggcagaaaa taccactaac aatagcgatt
     8101 atctgtatac gatgggggtt agtgccagat tttaacgtaa caatcacaat tgaaaccatt
     8161 gaataacaat agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat
     8221 aattgtcgcg tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat
     8281 gccgtttttc cccaggcata aagtgcactt cgttatggtt gtcggcagag atttttcctt
     8341 tttattactg caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggttga
     8401 cactcatcgg tactatcatt acccagtttg cgctggggtc ggtttatacc tggagcctgt
     8461 ttaacggcgc gctttccgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg
     8521 gcttgttaag tctggggctg gcaatatcgt cttctgttgc gggcaaattg caggaacgtt
     8581 ttggtgttaa acgcgtcacc atcgcttccg gcattttgct gggattaggg ttcttcctga
     8641 cagcgcattc tgacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg
     8701 cggatggcgc gggttacctg ctgacgctct ctaactgcgt gaaatggttc ccggagcgta
     8761 aaggtctgat ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat
     8821 ttatcgacac gcagctgctg gaaacggtcg gtctggaaaa aacctttgtg atttggggag
     8881 cgattgcgct ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg
     8941 aagtgaaaac cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta
     9001 aaccgcagta ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg
     9061 tgattggggt agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttctgcag
     9121 ccaatgcagt cactgttatt tccatcgcca acctttcagg tcgtctggtg ctgggcattc
     9181 tgtctgacaa aatcgcccgt atccgtgtta tcaccattgg tcaggtaatt tcgctggtgg
     9241 gtatggcggc cctgctgttt gcaccattga atgcagtgac gttctttgca gcgattgcct
     9301 gcgtggcatt taactttggc ggcactatta ccgtctttcc gtcactggtc agtgagttct
     9361 tcggcctcaa taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta
     9421 gcatttgtgg ttcgattatc gcctcactgt ttggcggctt ctatgtgacg ttctacgtca
     9481 tctttgctct gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga
     9541 aagtattgcg tgaggcgcat atttagccag aaactacagg ttttgaa
//
LOCUS       DAZGXS010000154         9406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGXS010000154 DAZGXS010000000
VERSION     DAZGXS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            71..1459
                     /locus_tag="ACHKG5_004087"
                     /pseudo
     CDS             71..1459
                     /locus_tag="ACHKG5_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            1479..2225
                     /gene="traX"
                     /locus_tag="ACHKG5_004088"
     CDS             1479..2225
                     /gene="traX"
                     /locus_tag="ACHKG5_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0870084.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2284..3144
                     /locus_tag="ACHKG5_004089"
     CDS             2284..3144
                     /locus_tag="ACHKG5_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0870085.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3247..3807
                     /locus_tag="ACHKG5_004090"
     CDS             3247..3807
                     /locus_tag="ACHKG5_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0870086.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3940..4152
                     /locus_tag="ACHKG5_004091"
     CDS             3940..4152
                     /locus_tag="ACHKG5_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0870087.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4397..4858
                     /locus_tag="ACHKG5_004092"
     CDS             4397..4858
                     /locus_tag="ACHKG5_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0870088.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4904..5113
                     /gene="hha"
                     /locus_tag="ACHKG5_004093"
     CDS             4904..5113
                     /gene="hha"
                     /locus_tag="ACHKG5_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0870089.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5151..5489
                     /locus_tag="ACHKG5_004094"
     CDS             5151..5489
                     /locus_tag="ACHKG5_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870090.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5729..5983
                     /locus_tag="ACHKG5_004095"
     CDS             5729..5983
                     /locus_tag="ACHKG5_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0870091.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6219..6293
                     /gene="tap"
                     /locus_tag="ACHKG5_004096"
     CDS             6219..6293
                     /gene="tap"
                     /locus_tag="ACHKG5_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0870092.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6286..7143
                     /gene="repA"
                     /locus_tag="ACHKG5_004097"
     CDS             6286..7143
                     /gene="repA"
                     /locus_tag="ACHKG5_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0870093.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            8056..8340
                     /locus_tag="ACHKG5_004098"
     CDS             8056..8340
                     /locus_tag="ACHKG5_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP0870094.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            8340..8615
                     /locus_tag="ACHKG5_004099"
     CDS             8340..8615
                     /locus_tag="ACHKG5_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0870095.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            8710..8916
                     /locus_tag="ACHKG5_004100"
     CDS             8710..8916
                     /locus_tag="ACHKG5_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870096.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(9016..9231)
                     /locus_tag="ACHKG5_004101"
                     /pseudo
     CDS             complement(9016..9231)
                     /locus_tag="ACHKG5_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9275..>9406)
                     /locus_tag="ACHKG5_004102"
     CDS             complement(9275..>9406)
                     /locus_tag="ACHKG5_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0870097.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         2431 a         2207 c         2613 g         2155 t
ORIGIN      
        1 gtatttttag tcatcctgtt tacctctttc tcagggagtt tagtctccag gatttccggg
       61 gcggttcaat caccggattg ccggtatcag cagggatgac gggctgataa ctctgcagga
      121 tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag gcgtcacact
      181 gtaccccccg gacaccatca gggtggggac cggtgaccgg atgcgcttca cgaagagtga
      241 ccgggggcgt ggttatgtgg ccaacagcgt ctggacggtg acagcggttt ccggtgacag
      301 tgtcacgctg tcgggcggac agcagacccg ggtgattcgc cccgcccagg agcgggcaga
      361 gcaacatatt gacctggcct atgccatcac cgctcacggt gcgcaggggg caagtgaaac
      421 ctttgccatc gcgcttgaag gtacggaagg cagccggaaa ctgatggccg gctttgagtc
      481 agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtacaccg ataaccgtca
      541 gggctggacg gatgccatta acaatgccgt acagaaagga acagcccacg atgtgtttga
      601 accgaaaccg gaccgggagg tcatgaatgc agagcggctg ttcagtacgg cgcgggagct
      661 gcgggacgtg gcggcagggc gtgccgttct tcgtcaggcg gggcttgccg ggggagacag
      721 tcctgcacgg tttattgctc cgggacgtaa atatccacag ccgtatgtgg cactgccggc
      781 gtttgaccgt aacggcaagt ctgccggtat ctggctgaac ccgctgacca cggatgacgg
      841 aaacgggctg cggggattca gtggtgaagg acgggtgaaa ggcagcgggg atgcgcagtt
      901 cgtggccctg cagggcagcc gtaacggaga gagcctgctg gctgataata tgcaggatgg
      961 tgtccggatt gcccgtgata atcctgacag cggtgtggtg gtgagaatcg ccggtgaagg
     1021 tcgtccgtgg aatcccggtg ccatcaccgg tggtcgcgtg tggggggata tcccggacaa
     1081 cagcgtccag ccgggagccg gaaatggcga gccggtcacg gcagaggtac tggcacagcg
     1141 gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgc gcagatgaaa ttgtccgtaa
     1201 aatggcagag aacaaacctg acctgccgga cggcagaaca gagcaggctg tcagggagat
     1261 tgccgggcag gagcgtgaac gggctgtcac ttctgaacgg gaagccgcgc tgccggagag
     1321 tgtactgcgt gaaccacaac gggagcggga ggcggtccgt gaggttgtcc gggaaaacct
     1381 gctgcaggag cgactgcagc agatggagcg ggatatggtt cgtgacctgc agaaagagaa
     1441 aaccctgggc ggagactgat acaggaagac aaacactgat gacaacggat aacacgaaca
     1501 cgacacgtaa cgattcactg gctgcccgga ccgatacctg gttgcagtcg ttgctggtct
     1561 ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg gtactggacc
     1621 acgcaaaccg cattctgcat ctggaccaga cgtggatgtt tctggccggg cgtggagcct
     1681 ttccgttgtt tgctctggtg tgggggctga atctgtcccg ccatgcgcat atcaggcagt
     1741 cggccattaa ccggctgtgg ggatggggaa ttattgccca gttcgcgtat tatctggcag
     1801 gctttccgtg gtatgagggg aatatcctgt ttgcctttgc agtggcggca caggtgctga
     1861 cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc cattctcctg atggcactgt
     1921 gggggccttt gtccggcacc agttacggca ttgccgggct gctgatgctg gcagtcagcc
     1981 accggctgta ccgggcggaa gacagaacgg aacgtctggt gctggttgca tgcctgctgg
     2041 ccgtgattcc ggcacttaac cttgcctcca gtgatgcggc ggctgtagct ggtcttgtga
     2101 tgacggtgct gaccgttggt ctggtgtcct gtgcagggaa atcattaccc cgtttctggc
     2161 ccggagattt tttcccgacg ttctatggct gtcatctggc tgtgctgggc attctggcgt
     2221 tgtgactggt atgatactga cgctgattct gcttattaat aaacaacata acacaggata
     2281 tttatgaaaa ttacggatca caaactctct gaaggtattg ccctgacatt tcgtgttcct
     2341 gaaggaaata taaaacatcc tttgattatc ttatgccatg ggttttgcgg tatcaggaat
     2401 gttctgctcc cttgctttgc gaatgccttc acagaagcag gattcgcaac gattactttt
     2461 gattatcgag gattcggtga gagtgatgga gaacgagggc gtctggttcc tgcaatgcag
     2521 accgaagata ttatttcagt tatcaattgg gcagaaaagc aggaatgtat tgataatcag
     2581 cgtattgggt tatggggaac atcgctggga ggggggcatg tcttcagtgc agctgcgcag
     2641 gaccaacgcg taaaatgtat tgtcagccag ctggcttttg ctgacggtga tgttctggtt
     2701 accggagaga tgaatgaatc ggagagagcg tcttttctat ccacgctgaa taaaatggca
     2761 gagaagaaga aaaacaccgg gaaagagatg tttgtggggg ttacacgggt actaagtgat
     2821 gatgaatcga aggtattctt cgagaaaatc aaagcccggc atcccgaaat ggatataaaa
     2881 atacctttcc ttacggtcat ggaaacgttg caatataaac cggcagagag tgcagccagc
     2941 gtccagtgcc cagttttggt tgttattgcc ggccaggata gcgttaatcc accggaacag
     3001 ggaagagcat tatatgatgc tgttgcttct ggtactaagg aattatacga ggaagctgat
     3061 gcatgccatt atgatattta tgagggcgcg ttttttgaac gtgtagtagc tgtgcagaca
     3121 caatggttta aacaatatct ttaattgtca gtatttatac gttaaaggtc gttgaaatta
     3181 acttcctgtt acgatgagcc gtttcaacgg cctgcagggc atattttcac gaaggtcaga
     3241 gtaagcatgg cagagcaaaa acgaccggta ctgacactga agcggaaaac ggaaggagag
     3301 acgcctgtcc gcagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg
     3361 aaaaagcaga agctggccga gaaagcagcc cgggaagcgg agctggcggc aaaaaaagcg
     3421 caggccagac aggcgctgtc catttatctg aacctgccca cgcaggatga ggccgtgaac
     3481 accctgaaac cctggtggcc gggattattt gacggtgaca cgccccggct tctggcctgc
     3541 ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa
     3601 aagctgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa
     3661 gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc tcaggaggag
     3721 gaagcgtatg cggcagcgcg tctggataaa atccgccgcc agaaccggat aaaggcagaa
     3781 cttcaggccg tgcttgatga gaaataaaaa aagcctccct cagagagagg aggcagggaa
     3841 ataaggctta aaaggaaata acttccgtac ccgaaaggaa gccaattcag tagattattt
     3901 aacaccataa tactgacggg ttaaagagga gtgataaaga tgaatggatt cagaaacagt
     3961 tcacggaacg gtcaggtctg gcgttaccag cgtgccggaa gccgggccgt tattctggaa
     4021 gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgccgg tgttgcaccc
     4081 cggactgact ggcagcagtt tgcccgaaaa agggctgaac actgccaccg gcgctgccga
     4141 ggaagggggt aacgaaaaaa gcagccttca ttactccagg gattcagaag gctgaataat
     4201 gcatgagatt tgcttttttt aatatccggt cgggcagtat gaccggccag ttctgagtag
     4261 cacagagttt tttccgtatt aagtcgtgat tttatatttt gtgattaatt tcacaaaata
     4321 aggtgttatt aagtgtgtgc tgcaatattc aggatgcctg aaatacaggt gctatttatc
     4381 tgatacggag aataacatga ggaaatatat tccactggta ttatttatct tttcatggcc
     4441 ggtattgtgt gcagatattc atggacgggt tgttcgtgta ctggacggtg ataccatcga
     4501 ggtcatggac tcacggaagg cggtgcggat caggctggta aatattgatg cgccggaaaa
     4561 gaaacaggat tatggccggt ggtcgacaga catgatgaaa tcactggttg caggaaaaac
     4621 ggttaccgtc acatattttc agcgagatcg ttatggtcgt atgctcgggc aggtttatgc
     4681 accagacgga atgaatgtta atcagtttat ggtccgggcc ggggccgcct gggtatatga
     4741 gcagtacaac acagatcccg tgctgcctgt ccttcagaac gaagccagac aacaaaaacg
     4801 tgggctctgg tcagatgctg atccggttcc cccctggata tggagacacc gtaaataaag
     4861 cgatggcaat cttatcattc atctgttctc agcgggaata aaaatggaaa aaacaaaaca
     4921 ggaatggctg tatcaattac ggcgatgttc atcagtcaat acgctggaaa aaattattca
     4981 taaaaacaga gacagtttat cgaccagtga acgtgaatca tttaattcag cagcagacca
     5041 ccggttagca gaattaatca ccggaaagtt atatgacagg ataccaaaag aaatatggaa
     5101 atatgtaagg taatagttca gcataattaa ttaccaggag cagataaaca atgaagctga
     5161 ttatttttat tctcattgtt ctgattattg cagcgttact tatccggatt atcctcagaa
     5221 gtgtaaatca gcattctcct ttgctgatgc aattgcatgc ggcaggtata cggaccggtg
     5281 atgcagagcg aatactgtcc ggcggtgaat atgatgccag ccatttacga cctgaacgca
     5341 gacgccggga tattcttctg gaagaggttc tgaggcaggc tggtattccg ttgctcagaa
     5401 gccacgatgc cagaaaactg ctgcagatga ccggagaatg gctgaataca acaggggctg
     5461 ctcagcagtc accggaacat cgtagctgac gccttcgcgt tgctcagttg tccaaccccg
     5521 gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc cataactgaa aaccatacta
     5581 tttcacagtt taaatcacat taaacgacag taatccccgt tgatttgtgc gccaacacag
     5641 atcttcgtca caattctcaa gtcgctgatt tcaaaaaact gtagtatcct ctgcgaaacg
     5701 atccctgttt aagtattgag gaggcgagat gtcgcagaca gaaaatgcag tgacttcctc
     5761 atctggcgca aaacgagcat acagaaaggg gaatccgctt tctgatgcag agaaacaaag
     5821 attatcagtg gcccgtaaaa gagcttcgtt caaggaagta aaagtatttc ttgaaccaaa
     5881 gtataaggcc atgctcatgc aaatgtgtca tgaagatggc ctgactcagg ctgaagttct
     5941 gaccgcactg ataaaaagtg aagcgcaaaa acgatgtgtg tgatgatggg cttacattct
     6001 tgagtgttca gaagattagt gctagattac tgatcgttta aggaattttg tggctggcca
     6061 cgccgtaagg tggcagggaa ctggttctga tgtggattta caggagccag aaaagcaaaa
     6121 accccgataa tcttcagaaa gtttggcgac tgagaagatt accggggccc acttaaaccg
     6181 tatagccaac aattcagcta tgcggggagt atagttatat gctcggaaaa gttcaagact
     6241 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     6301 caaacgtatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     6361 ggaacgctga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     6421 gatttcgcca ttcatgtggc acatgcccgt tcgaagggac tgcgtcggcg catgccaccg
     6481 gtactgcgtc gccgggctat tgatgcgctg ctgcaggggc tgtgttttca ctatgacccg
     6541 ctggccaacc gtgtccagtg ctccatcacc acactggcca ttgagtgcgg actggcgacg
     6601 gagtctgctg ccggaaaact ctccatcacc cgtgccaccc gggccctgac gttcctgtca
     6661 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     6721 accgacatca cgttcacacc ggctctgttt gctgcccttg atgtgtctga ggatgcagtg
     6781 gcagctgcgc gccgcagccg tgttgaatgg gaaaacaggc tgcgcaaaaa gcaggggctg
     6841 gataccctgg gtatggatga actgatagcg aaagcctggc gttttgtgcg tgagcgtttc
     6901 cgcagttacc agacagagct taagtcacgg ggaataaagc gtgcccgtgc gcgtcgtgat
     6961 gcgggcaggg aacgtcagga tatcgtcacc ctggtgaaac ggcagctgac gcgtgaaatc
     7021 tcggaagggc gcttcactgc taatcgtgag gcggtaaaac gcgaagtgga gcgtcgtgtg
     7081 aaggagcgca tgattctgtc acgtaaccgc aattacagcc ggctggccac agcttccccc
     7141 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     7201 cgccggtgca caaaaaatca gcaccacatc ccaaaaacaa cctcatcatc taccttctgg
     7261 tgcatccggt cccccctgtt ttgatacaaa acacgtctca cagacgggca attttgttta
     7321 tccacattaa actgcaaggg atttctctgt aagattacaa ccgttcatgt cataaagcac
     7381 cagccgccag gcttacaggg tgcaatgtat cttttaaaca cccgtttata tctcctttaa
     7441 actacttaat tacattcatt taaaaaaaag acttattcac tgcctgtcct gtggacagac
     7501 agatatgcac ctcccaccgc aagcggcggg ccccaaccgg agccacttta gttacaacac
     7561 acaaaaacaa cctccagaaa aaccccggtc ccgcgcagaa ctgaaaccac aaagcccctc
     7621 cctcataact gaaaaacggc cccgccccgg cccaaagggc cggaacagag tcgcttttaa
     7681 ttatgaatgt tgtaactaag catcatcatc gctgtcagtc ttctggctga acgttctcag
     7741 tacacgctcg taagcggccc tgagggctcg ctaacgcgga gatacgcccc gacttcgggt
     7801 aaaccctcgt cgggaccact ccgaccgcgc acataaactc tctcatggct gaaagcgggt
     7861 atggtctggc agggctgggg ggggggtaag gtgaaatcca tcaatcagta ccggcttacg
     7921 ccgggcttcg gcggttttac acctgtgcta catgtaacct ccgtataccg ccttacatgc
     7981 gctgacgcgc attatttgcc ttgttccgtc tgaaagtaat cactatgatt aaatatgatt
     8041 aacgactaat cggatatgca aatgaaaaac aataccgcac aagcaacaaa agtcattacc
     8101 gcgcatgtgc cattacctat ggctgataaa gtcgaccaga tggccgccag actggaacgc
     8161 tcccggggtt gggttatcaa acaggcgctt tctgcatggc ttgcccagga ggaggagcgt
     8221 aatcgcctga cgctggaagc cctggacgat gtgacatccg gacaggttat tgaccatcag
     8281 gctgtacagg cctgggcgga cagcctcagt actgacaatc cgttaccggt gccacgctga
     8341 tggaactgaa gtggagcagt aaggcgcttt ctgatttggc gcggttatat gattttctgg
     8401 tgctagccag taaacctgct gccgccagaa cggtacagtc cctgacacag gcaccggtca
     8461 ttctgttaac tcatccacgt atgggggaac agttatttca gtttgaaccc agggaggtca
     8521 gacggatttt tgctggcgag tacgaaatcc gttacgaact taatggccag actatttatg
     8581 tattgcgcct gtggcacaca cgagaaaaca ggtagattgt gtgtggggac aacttaacat
     8641 agcctgcaga tattaattct ggaacattat tatcaggcca gatatttaaa taatcttgta
     8701 ggagataaaa tgaattacga tgaaataacg aaaattaccg ctgaacgtat tagcgactac
     8761 atgactgaag cagttaatac agactctata gccgttgcgg aaatgtttca taacgcagca
     8821 tggggagtgc gaacattgtg gtttgaactc gtcacaaaaa tagatataga tatacataaa
     8881 aagaacagat atgcgagtta tgatcttgat cgataggaat ttaaatccca aaaagattaa
     8941 aaaaacaccc aaaaacggat gtttattcaa cactgcctgc gtgccatgag tgcaccgccg
     9001 tcaattaaat ggatctattt catcctgacc agcatagtgc tccagacggc tcatcggcac
     9061 cggcggtttt ttcaggtaag agccgaagta caccgccaca tgtgtggcgt tatccatcac
     9121 ccgggatacg ttgacattcc agccccgccg gtaatgactg tccaggaagc gattccagtc
     9181 ccgtttactg cttccttctg ctgccagcgc atccggtatc accaggtccg gtgaggtgta
     9241 ctggcaatag cggacactac catttgttct ttttttaagc agccatctga tgatattttt
     9301 ccctgaaggc tgccggggag atattcccca gacgagagtg acgacgctga cgattgtaga
     9361 aaatctcaat gtattcccgt attactgaga tggcttcatc ccggtt
//
LOCUS       DAZGXS010000155         9265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGXS010000155 DAZGXS010000000
VERSION     DAZGXS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..2346)
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004103"
     CDS             complement(<1..2346)
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP0870098.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGELTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGTLDVREKGSATGILQSTQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQSSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLAGTEARDST
                     VGNGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLMTPRDNVTSVKLEGAVRITDSATLTLGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTVAPATTNGIYNTLTTNELSGSGNFY
                     LHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGNTG
                     GFVDLGTYEYVLKSDGNSNWNLTNNVNPNPNPNPNPNPNPNP"
     gene            complement(2411..2530)
                     /locus_tag="ACHKG5_004104"
     CDS             complement(2411..2530)
                     /locus_tag="ACHKG5_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0870099.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2491..2676)
                     /gene="ymgJ"
                     /locus_tag="ACHKG5_004105"
     CDS             complement(2491..2676)
                     /gene="ymgJ"
                     /locus_tag="ACHKG5_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP0870100.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            2777..2950
                     /gene="ymgI"
                     /locus_tag="ACHKG5_004106"
     CDS             2777..2950
                     /gene="ymgI"
                     /locus_tag="ACHKG5_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP0870101.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            2952..3296
                     /locus_tag="ACHKG5_004107"
     CDS             2952..3296
                     /locus_tag="ACHKG5_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0870102.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3300..3635
                     /locus_tag="ACHKG5_004108"
     CDS             3300..3635
                     /locus_tag="ACHKG5_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0870103.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3691..6333)
                     /locus_tag="ACHKG5_004109"
                     /pseudo
     CDS             complement(3691..6333)
                     /locus_tag="ACHKG5_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
     gene            complement(6733..6951)
                     /gene="ymgF"
                     /locus_tag="ACHKG5_004110"
     CDS             complement(6733..6951)
                     /gene="ymgF"
                     /locus_tag="ACHKG5_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0870104.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(7083..8606)
                     /locus_tag="ACHKG5_004111"
     CDS             complement(7083..8606)
                     /locus_tag="ACHKG5_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0870105.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIVCAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(8990..9238)
                     /gene="ymgC"
                     /locus_tag="ACHKG5_004112"
     CDS             complement(8990..9238)
                     /gene="ymgC"
                     /locus_tag="ACHKG5_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0870106.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         2479 a         2231 c         2000 g         2555 t
ORIGIN      
        1 cggatttggg tttgggttcg gatttgggtt tgggttcgga tttgggttga cattattggt
       61 caggttccag ttgctgttgc catcgctttt caggacatac tcataggtcc caagatcaac
      121 gaaaccgcca gtattgccca gcgtaaacga agcatcccct ccccctgttt tcaccagcgt
      181 catcgcatcg tcagactgag ggctgacgcc ggtatcctga acaaagattt taaaattacc
      241 agtggcgttg ttgttgacga ccagttgatc gccccgggag cctgcaacgt tggtatgcag
      301 gtagaaatta ccgctaccgg aaagttcatt ggttgtcagc gtattgtata taccgttagt
      361 tgtggcaggc gctactgttt gtgctgataa ataaacatta ccgtcgttaa gtagcaaact
      421 gtttactcta tactcgcagt tgctggtgcc tgcacaggaa ttattgctgt taagccagac
      481 actgccccgg ctggcagccg tcaggtcggc aagcgttgta tcgacgccat tacccagagt
      541 caatgtcgcg ctatcggtaa tccggaccgc cccttcgagt ttaactgacg taacattatc
      601 ccgtggcgtc attaacgaca ggcttcctgt cgcgccactg accgatgcat ccgccagcgt
      661 acctgcgtag acgattgccg tcccgccagc gacctggaga tcttctgccc gggccagagg
      721 ctgaaactca tcttttgacc gcccaagggt atattgcccg ccagagttaa cctttgtggc
      781 ggagtcctga cccaggtttt gcattgcccc accgttgcca accgtggagt cacgggcttc
      841 ggtacctgct aataccaata aattaccgcc attttccagc aacatattgg tcgctaaatt
      901 gccggcaatg gaaaaggtac cgtactggtg agtaccgctg atttcaatac cgttagccgt
      961 gctcgtctgg agagcggcac cgctgctttg tacgatatct gtcgctttgc cattatcgtt
     1021 gactgtcagc gtaccgcctt cattgatcgt tgtttttatt gcctcaccgt tagccgaaac
     1081 tgtttgtatt ccgccgtcgt taatcgttgt ctcattcgcc acaccctcga cgatttgttc
     1141 gccaccggtg agcgtcgtgc ctgtcgcagt ggcttttgtt ttgacgatct cccgtccgcc
     1201 cgtattgacc tgtgttttgg cggaagtggt gtctgactcc acggttaaaa cgccgccatt
     1261 tgccagcagg atattgttcg ccgcaccctg ctcgatgctg aacgcgacgc catcagcgcg
     1321 tgtccctgtg acacgcgtcg ccctggtggt tgcaaccaac gcgccctgag tactctgcag
     1381 tatccccgtt gcgctgcctt tctcccgcac atcgagtgtg ccgccattgt taagtctggt
     1441 ggtatatgct acggcctcct cgtggacaat cagcttaccg ccagcatcaa cgatggtatc
     1501 taccgccgag gtttgagcta cgaccgtcag ttcgccggta ttttccagca caacatgctc
     1561 agccctgcct ccggtaatag tgaagtgaga tgacctctgg tatccgtcaa tatcagtccc
     1621 cgcgcccgtg ttggcaacta aagcactccc cgtgtccagg taaaccccat gtgcgatacc
     1681 gccggtatcg acaatcagcg tgcctccggc gcgaatattg ctgccagttg ccgtacctcc
     1741 tttctcaaca acctgcttgc tcccggaaga gatatttgtt gtgtcagctt tcccgccgct
     1801 cttgatattt tgcgtgccac tgttgatatt ggttcccgta gcaataccat gattataaat
     1861 attctgtgtg ccgccattaa ttatggtatt agtcgcgttc ccggcaacat ccatggcccc
     1921 gccgttatct attcgggtcg catcagcttt agcattcgtt aaaactgaca ttgttccttt
     1981 atcgttaatt atcgtccctg tagaaattcc tccctcacta attgtctggc tgccaccgtt
     2041 aatgactgta ttattagcct gcccctgata agagccatga ccagatacct cttgatgtcc
     2101 accagtatca ataacacttc cattagaaat cccgccatat acctcctgat tgccgtgatt
     2161 gatgatatga gcattatttg ttgtacctcg ttcatctact ctttggctgc cgtctacagt
     2221 ctcatcattt acaacgccaa taacattaga agtgaaggcc gtcatcccag gcggggcata
     2281 tatcatggca gctatcaata aggaaagtac tgagcggtga aaataatagc gactgattct
     2341 gttcataaat ttcatcctct gaaaaatgaa tactgagtag cgtctaagta acctaagctc
     2401 ggctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcag
     2461 gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat cccgcataag
     2521 aaagaggcat tttttaaacg cagtgcgctg aagtgtggtt ggataaaaaa tcactctttt
     2581 cagataatac tgtcgtacgc ttatctttcg acaatgaggc cgtcggcaaa ataaaatgac
     2641 taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca ataatattga
     2701 taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac ttcttgaata
     2761 aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc acgtaaaaaa
     2821 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
     2881 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
     2941 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
     3001 ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
     3061 ttgggttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gtaggcgctg
     3121 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
     3181 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
     3241 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
     3301 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
     3361 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
     3421 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
     3481 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
     3541 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
     3601 ttgatgacac catgcgtgct atcggcaaaa aataatattt aatatgttac cgcgtcgatg
     3661 aattcgacgc ggtattttag ctaacgggga tcagaactta tatttcaggc ccaccatcac
     3721 tgcggtgtca ttgtagccat tatcacccaa ctgcacgccc acattacccc agacactggc
     3781 cgccggattc agattgccat ttacgccaag tttaatttcc cccagattac gcgccccgtc
     3841 ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt
     3901 catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg
     3961 aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg
     4021 tgagccattg gcttccgtat gcgcattatg atcgacgccc atccagatag cctgtggctg
     4081 gaagatccac gtatatttcg ccgcttccag accaaaggat ttattcaggg caaagccgta
     4141 acccgcttcc agcgagacgg ttgcgccttt cgcactatag gattcatagg ctaattcatc
     4201 ccctttcacc gagtttttaa accagctata ttgcgcccag gcgtcaatgt atgcgccttt
     4261 tttactgatg tcatcggcaa accaggtggc atacagccct gcgctatagc ctctgacact
     4321 ccctttcgaa cgataatccg acacgctgga atgagttgag ttgtagtcgc gggcataacc
     4381 agccatcact cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta acagatcggc
     4441 ccctaactgc gagacgtagc gatgcgaagt tgttctcaac tgtccgttgc tgtcacgcca
     4501 ggcattatgc ccgccaattt gacgtagcca aagtcggctt gaacgctcct gttcagttac
     4561 aggatcgatg taacgcgttt cacctgcacg atcgttcaga cgcataacaa aaagattgtt
     4621 agcggctgcc aggttggcaa cgtaacttcc agcttcgggg cgcaggacgg gggtgggcgc
     4681 aacagtaggc gctgaacttc ctccggagtt tgtttcaccg ccgctcgttg cctggaagtt
     4741 agtcagatac cagtttttat tggtcgcatc agtattacct cgggttaatg aatattcgta
     4801 ggcaccggcg aaaatcctcg aatccttaat aaattctcca tttgattccc cctcaacgct
     4861 gataatctct atcccattta acgtgtaagc cccgctgccg ccagcattgg taactttaac
     4921 tcgagttgtt ccagaggtat ttccattaat aacgacttta tccgtagctg aattatcatc
     4981 acccaattca gttctgagat gcaatacgcc attattacca acgtaatttt cagttattgt
     5041 taatcttgtt ggttcgaaat cccttccatc tgcccgggaa atatagaccg tactattatc
     5101 aaccgtgagg ttgctcaccg ttgagtcagc tttaatatcc caggtactat tatctgaaag
     5161 cgacagatag gtatgagtgc tatcatcttt tgaaagattg gctgaacccg ttatttttga
     5221 attagtggca tcgatggcca aatgaccttt aatattattg ataaaaacat ctgcagccga
     5281 tgatacctca ctatccgcga tggtcacgtt actcgtctca tttttattgt agaggatttc
     5341 atttgctgac cgtagttagc taccagataa aatatctact gttgcttcct gaccatttat
     5401 ggaagactcc agcacaactc cagccccctg atgcgcgact gcagatgtat ttttcagcgt
     5461 caccgtgccg ccattataaa ttttagcaat cgcggcgtca ggacccgttg tataaatttc
     5521 tccctggtta aatgaaactg tcccctggcg agcagcgatg gcatgagcat tgtccccatg
     5581 cgtggtgata tcaacattgg ttaatgtaac gctggcattg gtataatcaa gcttgtcacc
     5641 agacttgacg acagtgccat tagcgtaagc ggcataactt tctgttccgt gggttgttat
     5701 ctcccctttt gccatatcca gtattgcttt ggcgctattt tcattacttc ctgtatcggc
     5761 agtactttct tcatcaagag aagatacaca cgcacgcgcg cttccgggag atgcacacgc
     5821 aagaacgccg actgcgtgat tacccgcagt aacgataaaa gtattacctc cgtcagaaaa
     5881 agagggggta gccgaaccgt cagggttgac tcttattatg gtatcagtat tcaggtttcc
     5941 attcgtttga cttagcaaac cgattgagta atcccccgtt gtgacaatcg tattattacc
     6001 ccgaaaagtg acagcaccgc catattcact tacgactgcg ctagctgtat atgctttgct
     6061 tagagaacta ttgtcagaag gatataaacc gcccgttgtt acaatgacgc cattgttaat
     6121 ggttaacttg ctaccctctc ctgcaacaga taccccgtaa cttccattac ccgttgttat
     6181 aactagcgca ttattaatat tttctgtatt tgcactatct attttcagcg catgttcctt
     6241 atcttcatgc tgaataaacg gtccagcata tgcattaccc accgccagag caataagtcc
     6301 taaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     6361 caaacattcc tggtcaaata acctatgagt tcagattacc aaaacggagg gtaaagacaa
     6421 aaacaaaagc acaaaaaatg taaatagata aatagcgaca atatataaat gataaataag
     6481 tcggcattat catttttcac cttattttca tgacattgat cactttgagg tgacgatttt
     6541 gtttaacaaa taactcttcg acatcacaat cattgttagt tgccatagaa attatttcaa
     6601 tgatatataa aaacaactgt gataacacca gggttgacaa ctatttgcgc aataattcct
     6661 ttccttcaat gatccaggcg ttcactgtca gcaccccatt cagccgcata ctgacagtga
     6721 gaaactgaaa gattattgct gatccctgtg gctgacgagt ctttccctta atcgattaac
     6781 cagattgatc gccagccaaa caagggcatt aagcaaattt atcgtcacaa gcacttctcc
     6841 cagcccggta ctgggttttg caataaggat gaccaggaga gtgatcagca taaatgcaat
     6901 ggaaaagatg atatagagag tgaaataatc cagattatta ctattgttca tgctactaat
     6961 ggcaatcctg tcaaaacttt caaacaatta tagcaaacaa aataatgaac aatagtaaca
     7021 agaggcaacg agctaattag ctgtctataa ttacaccgca ctatggcctt actgatcgat
     7081 tttcactcaa ccacaacctt caccttcggt ttagaaagga taattttgac caggtcgatg
     7141 tatgtaacag gtttatagaa ataataaccc tgctgaaatg tgatattatt ttggttcaga
     7201 taatcaagtt gttctttcgt ttcgacacct tcagcgacga tactgatcga aagtttacgc
     7261 gccagatcca atacacaatc cagaattcgg gttgattctg ggtctgcgtt gacgcggcca
     7321 acgaaactgt gatcaatttt gatataatca atatgcaggt catgcagata agaaagccct
     7381 gagtagccag taccgaaatc atccagcgcg atgacaaaac cattttcatg cagaatgttc
     7441 aaccgctgaa ccagactttc atcaacattt aatggctcac gctcggtaac ttccagaacg
     7501 aggtttaaat cgcggcgggt aaaactgtca cggtaattta agcactcgtc gacaaacgtc
     7561 ggcgaaataa tatgcgaggc gctaaaatta attccaatat gaaaaccttc cggcaattta
     7621 ctcgcgatag cgttcatctg tctggcaacc tgattcatca ggctttgcgt aagcggaacg
     7681 attaatccgg atttttcagc aagtggaata aatgccgcgg gtgaaatata tccaccgtga
     7741 ggttgtttcc agcgggctaa cacctcaact ccccgcaatg tcccttcccg accattcacc
     7801 actggttggt aaaaagggac gatctcccca ttatttatag cccgccgcag gatctcttca
     7861 ggcgttgcgc ttttattgaa atacctatct agcaggaagg cagcggcgca gacgattaac
     7921 agaataaaga tcaaaatccc aaagccttta tcgataagtc tgtagaagct aaagagtggc
     7981 ggcgagttaa agataatagt gaaatgatat tttgtggagt gaaccttctc ggtataggga
     8041 taatgtccat taagcgtcat cacatcaccc gtaggtccaa taatgtcatc cgccacgcgt
     8101 aatacatacc ttaccccttt caagggtaca ttaagcgccc cgcgaatatg ctgatcactt
     8161 atcgtaacca aaatgcgcgt atctgcaaat tggctctgat agagcaggat aggtaatcta
     8221 ttaacggtgt cgattgccgg agccaacagt aaattactgt ccgggaaaac aggaatacga
     8281 gacagcagga cccgattccc cggtaaggat gtacaccaga ccgttccctg tttgagaatg
     8341 atgattgtgc gcaggtgagg tttcagagct gcttcagtgc caagctgata ttgctcctcg
     8401 aggtcgcatt ccttcccggc aatgttaacc gctgtttgag tcgcctgtga cgcttcttca
     8461 agtatatgat tgatattgtt ggcggcatat cttgctcccg ccaggtactc tgcttttgcg
     8521 gaataccaga gctggatgtt caaaatagcg acaccggtta taaacaagca tatcgcaacg
     8581 aggatgggta tgagtgtatt gcgcattcgc ttgtcccgaa caaagggttt aatacatgaa
     8641 aatgacgttc attcgctcgc gctggcgtct acagagagca tggtagtgag ttcacagata
     8701 aaacactctc caggaaaccc ggggcggttc atagagagca taagaaatat ccacgccgaa
     8761 gataaaattt tctccatctt catctaacct attaattatt gagcacaatg taggcagaat
     8821 taaacaaatt aggtgggata tcaggcgtca agaatggaaa acgagcgctc tccattcctg
     8881 tttgccccct gatattgcgg atatattaag aaagcataac gcctgaaatg ctcactttgc
     8941 accagcatga tgatacagtt gatgtttatt ctataacctt actcaagttc taagagagca
     9001 cggattccct gtcattaata atgatatccg ttagtgtagt cttatgcatt tcaatgtact
     9061 gcaagatttg cagatagaga tcagaaagaa tcacactggt ttcatctttt aaacgcaagc
     9121 gtaaagcctg atcaataata tgactatcaa catacccatg cgtcatgaac acctccctct
     9181 cgagattttt aaacaaagca cattgaaaaa taaatctctc tgggattgaa ttattcatgt
     9241 cagcctcgta gaaatattaa cccaa
//
LOCUS       DAZGXS010000156         9222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGXS010000156 DAZGXS010000000
VERSION     DAZGXS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            65..208
                     /locus_tag="ACHKG5_004113"
     CDS             65..208
                     /locus_tag="ACHKG5_004113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870107.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(427..1965)
                     /gene="aldB"
                     /locus_tag="ACHKG5_004114"
     CDS             complement(427..1965)
                     /gene="aldB"
                     /locus_tag="ACHKG5_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0870108.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2073..3368)
                     /locus_tag="ACHKG5_004115"
     CDS             complement(2073..3368)
                     /locus_tag="ACHKG5_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP0870109.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3498..4649)
                     /gene="yiaY"
                     /locus_tag="ACHKG5_004116"
     CDS             complement(3498..4649)
                     /gene="yiaY"
                     /locus_tag="ACHKG5_004116"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0870110.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(4838..6682)
                     /gene="selB"
                     /locus_tag="ACHKG5_004117"
     CDS             complement(4838..6682)
                     /gene="selB"
                     /locus_tag="ACHKG5_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0870111.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(6679..8070)
                     /gene="selA"
                     /locus_tag="ACHKG5_004118"
     CDS             complement(6679..8070)
                     /gene="selA"
                     /locus_tag="ACHKG5_004118"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0870112.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8168..8776)
                     /locus_tag="ACHKG5_004119"
     CDS             complement(8168..8776)
                     /locus_tag="ACHKG5_004119"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0870113.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            9005..>9222
                     /locus_tag="ACHKG5_004120"
     CDS             9005..>9222
                     /locus_tag="ACHKG5_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870114.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPF"
BASE COUNT         2165 a         2545 c         2294 g         2218 t
ORIGIN      
        1 ctgcgtggct ttttaacagg tcgaggctta tcatattgaa ataatggaac tatcagccaa
       61 actaatgaat aacttcagta aaacatccac aatgcagatt aagcgtatta aaccttcaat
      121 aatctaccgt ttattgctga ttgtcctcgg aataccaatg gtgatttacg gtctggtttg
      181 cccgctcacc atcgaaacgc gagattaaac tcctggcaaa acacgcccca gaaacgcact
      241 tttgttggcg tagtctgggt tattgcacac gtaggtttaa aacgtaaatt gtaacaacgt
      301 gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt caggtcccca
      361 taggcatgat aagacgcgta agcgtcgcat caggcaatta atacccgatg cgaccagctt
      421 cttacatcag aacagcccca acggtttatc tgagtagctc actagcaggc acttggtttg
      481 ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt gtttgtagcc
      541 accaaacgcc gcatgtgccg ggtaagcatg ataacagttg gtccacacgc gcccagcctg
      601 tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc ccgcgcccag
      661 gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga aggtagtcac
      721 cgccagcacc gggccaaaaa tctcctcctg gaacacgcgc atattgttct gaccaaacag
      781 aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct tgcgccgccc
      841 gccggtgagc acatcagcgc cctctttttt accgatatca atgtagttga ggatggtttc
      901 cagttgcccg tgagaaacct gcgcacccat ttgcgtcacg ctgtcgagtg ggttaccgct
      961 gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga ttgactcctg
     1021 cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa acagtgcaaa
     1081 gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag caaagaagat
     1141 atttggcgac ttaccgccca gctccagcgt caccggaata atgttttgcg tggcgtattg
     1201 cataatttgt tgacccactt ccgttgagcc ggtaaacgcc actttggcga tgcgtttcga
     1261 ggtcgccaga tattcgccaa tttccccacc tgcaccgttg accacgttca ccacgcccgg
     1321 cggcagtaaa tcaccgacga tttccattag cagcagtaca gaaagcgggg taagacgtgc
     1381 gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc agctcgccat
     1441 cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt catggaaatg
     1501 ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc gaatacacga
     1561 ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg tttcgcgaat
     1621 gggtttgccg ttatcccagg cttcagctgt cgctaacagc tcgaggtttt gttccattcg
     1681 atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg cccatttatc
     1741 tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg aagacgccac
     1801 ttcgcacagc agctgcccgg tcaccggcgt caggttctgg taatactcac catcggcagg
     1861 ggctacccat tcgccgccaa taaagttgtc atagcggttt tttaacttga gggggaaacc
     1921 atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg tctccttgcg
     1981 gtgtgaggta taacaagggt agacgtggct ggcgaaatct tcgccagtcg gtaacagctt
     2041 ttcgacggct atcacgaatt tacgggcaag ggttacagcg gataatgcgc gaagtacacg
     2101 gcttgctggt cattaatttt cttctcttgc gcaaaccctt ctttaacaat tgtccgtaaa
     2161 tcagctcgaa ccgtattatc agagacgccc aggctcgcgc taatttcagc aactgtgtac
     2221 tctttccccg gattggcgag catcacattc aggagcgtta cctggcgcgg attcaggcgt
     2281 ttaagcacac cagagctaaa cagcacacta tcgagttttg cgcttcgttg tgtaatttca
     2341 tcgatatgct gctgccagtt ttgcagagca cgtttaatga cacccgcctg atattcaagg
     2401 aaataagtca gatccatacc atctgattca gtgtactggt agctggcggc atatttgacc
     2461 ggtgcagcat gaagcaaacg gctgatggaa atatatttaa agacatcgta gcccgatttc
     2521 agcatatacc agtaaaacag cgcccgactg gtgcgcccgt tgccatcccg aaacggatgt
     2581 tcatgggcca gcataaaatg cagaatgcag gcacggacca acggatgaat atacccttca
     2641 tggctgttta accactggca aactttttcc agccgttccg gtaacaatgc cgcagcgggc
     2701 ggttgatgca ctatattgcc atcatagtcg gcgatcacaa catcatctgt ttcacgaaat
     2761 tctcccggac gatatttcgc gtcattgatt ccacccatac caatggcatg taactggcga
     2821 ataagcgcag gtgtcagcgg ttctgcgagt aagtgcggaa tttccgccat cagccgggca
     2881 tttcccgcaa tcatatgctc gtcttcagtg cgtggcgacc gtcctgtttc cagcattttt
     2941 ctcgccacca gcgttgtagt attcgcacct tccagctggg agctggtgat cggctcttcc
     3001 aggcgtaact ggcttaactc ggctccggcc ccgcgtaacc gttcagtctg atctttcagc
     3061 gccagcgcgg tagcacgttt gtcgacaagc tcgcacgctt ctgcaatcac cggggtaatg
     3121 ttgaatccgg cctgttcacc cgcttcattg caataattaa ttcgttggat cgccgcgttg
     3181 cgcgccagac gggtaagcgt ccacgccaca tcgacgtttt ctcctttttt gatgcggtac
     3241 tgcaactcat caaaaggcag ataacgtcct ttttcatcgg taacccggtt aaagcgcagg
     3301 tgggcctgga gttcctcacc cgataagtgt gataaaggaa tcagcgtggg aggtgatttg
     3361 atagccattt gcttatctca aaaaagcgca atattgagat agtgtatagc aggaaagaga
     3421 aactatctca aaaaaagcaa gttttgagat agttgaaaaa atggctgaca atttgccagc
     3481 cgttgtggaa atgatgatta catcgctgcg cgataaatcg ccacaatttc ttcgtgagtt
     3541 gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagccgc cagaaccgcg
     3601 aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac tttcttcgcc
     3661 agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt acctgtcacg
     3721 ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt gctgttgaat
     3781 acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt gtagaaaccg
     3841 cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa cgccataccg
     3901 gcgaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc atcttcaacg
     3961 gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc gtcagtgatc
     4021 ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc catacccgtt
     4081 gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac agaaagcagc
     4141 ggagtgacat gtttatcaac aatcgccatt ttgatatgac gcgcttcgtc agtgatgatg
     4201 cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat catcggcagc
     4261 tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc attggctgcc
     4321 accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga gatcacgcta
     4381 tcgcaattat tctctttaag taatttcaaa cctgcggcga cgttttccgt ggtggggtta
     4441 ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc tttttgcaca
     4501 tcgcccgcca tacctaattt cgttaacata ctgtcagtga caattaaggt acgggtaaat
     4561 ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat gacattcaca
     4621 gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa aaaagggtgg
     4681 gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta tcaaaattaa
     4741 tctaataaaa agcacattgt attcattaaa caatgtgctt ttttaaaatc ataagaaatt
     4801 tgtattaatt tattttaaaa attaattaat catttcctta tttttccgga aataataatg
     4861 cgtcgcgcaa taaatgatca tttccacgac gacgcgtaaa gccaatgcgg tcaaaatatt
     4921 ccagaatctg aattgccagc tttcggccta cgcctaagcg atcgcggaaa tccgccgcgc
     4981 aggttgaacc acactcctga tcgagatcgc ggatcatatt ggcaaattcg acaatccgat
     5041 cgttacggta ataacgatct ttgacgatcg cggtaattat tccttgctgc gccgcctggc
     5101 gtagcgtcag gcgcattgcc tgctcgtcgg tacccgtctc ttttgccaga tcacgcaccc
     5161 accacggctc gtcaccaaac agcggctctg ctttttgcca gatggcctgc tgctcttcgc
     5221 tgaagcccgc tttgtgatcc ggcagatgca gccagccgtg atgactgagg atgtcgccgc
     5281 tttcgcgcat cttttcaatc agcaacagta ctagcgcttc atcttccatt ggcaacgcca
     5341 tacgtcgcag acgttcgcgc ccaggcccag gttcatcgcg atgttgctca tgataagtcg
     5401 ctaatgtgtc gagaattttc cgttgccagc gagcggcaac cggcgcattc aacaagctat
     5461 aaccagcctg aatatagcca ggctgttgca gcagttcgcg catcccttcg ccgttgagct
     5521 ggcgcgccca ggcgaaatca ggaagattaa ccgcgccgcg ttgcaggtga acggctaacg
     5581 cttcgctatc attctgtgcc ctggcgaggg aagccaggca ttgcagatac tctggtttac
     5641 gtttaccgcg acgcggcggg ttaagcatca cgacgcgcgc tccggccagc gtgttgcggg
     5701 cagagatatc gcgcaatacc aggcggtcgt tatctgccag ccataacggg gggtcgaaga
     5761 ccagttcagc aaggttatct tccagcagtg aaacgcgtcc cgtgacgtgg ctggcggcgt
     5821 ggtgaatatg cagcggctgc cactgggtca gcggtgtatg ggtttgaagc tcgacaatca
     5881 cccgtgtgaa cggctctggc ggcacatcgg caagcagcca gtcgccacgg ttaatctgct
     5941 ctttttccgc atcacccgcg atgttaagcg cgatacgctg cccggcattg gcggtttctg
     6001 ttggctggtt ttgcgcatgc agcgcacgta cacgcatcgg tttatttaca ccagtcagcc
     6061 agagtgaatc gcctaccttc acttccccgc ttaacgccgt accggtgacg accagcccgg
     6121 caccttttac ggtaaatgcg cggtcaatcg cgaggcggaa actatgttgg ctggcgtgct
     6181 cgcgttccgg caactgaagc agatgctcgc gcagggcatc aattccccga ccttcggttg
     6241 ctgcggtgat aaacagtttt gcctcagcaa aaccgtattc ccgcagaacc tcctttacct
     6301 ggcgttcaac ctcatcaaca cgcgcttcgt ctacgcgatc ggctttggtc agcgccactg
     6361 tcagcatcgg gttaccggtc agctgcaaaa tcgccagatg ctcacgggtc tgtgccatca
     6421 cgccgtcatc gcacgccacc accaacagcg cgtgatcgat accaccaacg cccgccagca
     6481 tgttggaaag aaacttttca tgaccgggta cgtcgataaa accaggcacg cgaccatccg
     6541 gctgcggcca gtaggcatag ccgagatcga tggtcatgcc gcgctttttt tcttccggca
     6601 gacggtcagc atttacgcca gtaatcgcct gcaataaggt tgttttgccg tggtcaacgt
     6661 gtccggcagt cgcaataatc atttcaacaa catctccaaa aaccgttgct catcttcaag
     6721 gcagcgtaaa tccagccaca atcgtccgtc ataaatacga ccaatcaccg gcactggcaa
     6781 ttcacgccag cgggcggcta atgactcaag gtggctaccg cgtccatcat ggggtgtaaa
     6841 cgttaatgcc gcgctcggca ggcgatcaac cggcagcgaa ccactgccaa tctgcgaaag
     6901 acatggcata acctgtaccg caaactccgc gccgtaatgt gcggcaaggg gagcctgtaa
     6961 acgttgtgcc tggatttgaa tgacctctgc gctgcgggta agcaggcgca gggtcggtaa
     7021 tttttcactc agagcttcag ggtgtaaata aagacgcaac gtggcttcca gcgccgcgag
     7081 ggtcatttta tccgcgcgta atgcacgctt cagcgggtgg ctttgcaggc gggcgatcat
     7141 ctctttttta ccaacgataa tccctgcctg cggcccgcct aacaacttgt cgccggagaa
     7201 gctcaccaga ctgacgcccg ccgcaatcaa ctcctgcggc attggctctt tcggcaaacc
     7261 gtactggcta agatcgacca gcgagccact gcctaaatca gtcactacgg gaacatccag
     7321 ctctttgccg agcgccacca gttccgcttc atctatcgct ttggtgaacc cctgaatgct
     7381 gtagttactg gtatgtactt tcatcaacag tgcggtattt tcattcaccg cctgacgata
     7441 atcattcgcg tgcgtgcggt tggtggtccc tacttcgtgt agggtgcagc ctgcctgacg
     7501 cataacatcg ggaatacgaa acgcgccgcc aatctccacc agttcgccgc gagataccac
     7561 cacctctttt ccgctggcag tggccgccaa catcaataac accgccgccg cattgttatt
     7621 gacgatacag gcatcttccg cccccgtaat acggcacagc agctgcgcca gcgcccgatc
     7681 gcgatgtccg cgtccggcgt cgtccagatc gtactcgagg gtcactggcg aacgcatagc
     7741 ctgcgcaacg gcttccaccg cggcttccgc ctgtaaagct cgcccaaggt tggtatgcag
     7801 cacggttccc gtcaggttga tcaccggacg cagcgcgctc tgcgcttctt tcgtcaaccg
     7861 ggcatcgact tcttgcgccc agttttcaca ccacgcaggc agcgtctggc tgccacgaat
     7921 cacttctcgc gcttcgtcga gcatctgacg caacaattcc accacgcggg tgtgaccata
     7981 agtatcacgc aaagaaagga aggagctatc gcgcaataag cgatcaatag ccggaagttg
     8041 actatagagg gaacgcgttt cggttgtcat aggaaacctg gctgatcaag gccctctcac
     8101 acggagaagg gcgtttaaca taaccacgga ttgtaacgtg agatgggtca ggaggacata
     8161 tcgcgcatca agcctttggc ggttcggtgc gagcaaaact ttcgcggcta aacaggtttt
     8221 cgaccagttt gactaagtgt gggcggtcga cacaccaacc tggcgcaacg cggcggaaat
     8281 tgagatatcc gacggcacag gcaatggcga tagtcgccag attgaccgta tcggttttga
     8341 gtgtgccatc gaccagatat ccttccagca catccagact gcggttgatt ttctcccgct
     8401 ggcgtaacaa ttcatcttca gactgctgcg ccgctggacg cgcctgttca cgcaccgata
     8461 ccagcccggc atccataatg ccatccgcca gtgcctcaat tttgcgcacc cgcaacgact
     8521 ccagcggatc gcgcggcaac atcgccggag cgacattcat taattcaata tattcagcga
     8581 tgatcggcga atcaaaccag cattcgccct cttcggtcac cagcaccggc acttttccta
     8641 acgggttaaa ttgcgtcacg ccgttgtccg cgttataggg cagttcatta ataaattcga
     8701 aagttatgcc cttttctaac aacagaatag aaagtttgcg tacaaacggg ctggtgtagc
     8761 taccgacgag tttcatgccg agtcctttgt gcgaggaaaa atatcagtat ggcctggtga
     8821 tgggaaaaag ggcagaaaat gttgatggtt tgttacttcc aaataaatca catatttatc
     8881 atggtgatat aaatattttc ctaattgttt ctatctgatg gatatctcac ttaaggcttt
     8941 cttataaatc tgtagggttt tgcctggaag cagacaaata acccgataaa acaaggatga
     9001 gcagatgagc ggaaaaccgg cagcgcgtca gggcgacatg acgcagtatg gcggtagcat
     9061 tgttcagggt tcagccgggg tgcgcattgg tgcccccacc ggcgtggcct gttcggtgtg
     9121 ccccggcgga gtgacgtccg gccatccggt caatccgctg ctcggtgcaa aggtccttcc
     9181 cggtgaaacc gacatcgccc tgcccgcccc gctgccgttc at
//
LOCUS       DAZGXS010000157         9196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGXS010000157 DAZGXS010000000
VERSION     DAZGXS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(88..411)
                     /locus_tag="ACHKG5_004121"
     CDS             complement(88..411)
                     /locus_tag="ACHKG5_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0870115.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(419..604)
                     /locus_tag="ACHKG5_004122"
     CDS             complement(419..604)
                     /locus_tag="ACHKG5_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0870116.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(601..3240)
                     /locus_tag="ACHKG5_004123"
     CDS             complement(601..3240)
                     /locus_tag="ACHKG5_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0870117.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3448..4437
                     /gene="ldhA"
                     /locus_tag="ACHKG5_004124"
     CDS             3448..4437
                     /gene="ldhA"
                     /locus_tag="ACHKG5_004124"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0870118.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4548..4970
                     /gene="hslJ"
                     /locus_tag="ACHKG5_004125"
     CDS             4548..4970
                     /gene="hslJ"
                     /locus_tag="ACHKG5_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0870119.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4967..5233)
                     /locus_tag="ACHKG5_004126"
     CDS             complement(4967..5233)
                     /locus_tag="ACHKG5_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0870120.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5507..9031
                     /gene="nifJ"
                     /locus_tag="ACHKG5_004127"
     CDS             5507..9031
                     /gene="nifJ"
                     /locus_tag="ACHKG5_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0870121.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2292 a         2413 c         2259 g         2232 t
ORIGIN      
        1 acccacggta attaagcatg ctaacgtcag tttaaatact gtaaaagatc attacattaa
       61 tagatacatt ttgtagccaa ctataattta tttgcgcaac catttaccat taactccctg
      121 aacatattgc cccgacttcg cgcgagcaac aagcttttgt ccagccagtt tggctatctc
      181 tttagtggat acattatttt gctttgccag ttgttggtaa ctatggttac gagcttcatt
      241 aatatctttt actaatgcct gtgtttcggc atccgtttgt agcgccacca ggtaaccgtt
      301 aagcgtttca cctacccgcc cctgacttct ggcttcgttc agggttaaag ccattgcact
      361 gtcgctcatc aggccaatgg cccatacagc tatgagtaat gttcttttca tcatcacctc
      421 aaaatagatc gctacgggtt ttgagcaggt cttcaacgtc cttatccgct ttaatgatga
      481 tttcatgctc aattttaacg ttcatattaa tggtgattgg ctctttcggc gcagccactt
      541 caatgcgagg cgtgcaacca accaacataa aagaggacgc aaacgcggct atcactattt
      601 tcattgtttt tcctcacact cttttcctga tgggcagcca gtttccggta gctgggcatt
      661 ctgctccagc catacctgga gattatcgcc aaagcgtaaa ctgcgccata acgtaaaaat
      721 attctcttca tggtgataat taaggtttac cgtaccgctt ttaccatcga cgcgactggt
      781 gcccgaaatg ttggcctgca tggttaacac accgagatta tctaaattaa tccttgttga
      841 tgagcgactg atttccatat aacgcaacca gttgatggca gttccagcgg tcatattgtc
      901 tttggccacc gcatctgcag tgtctttatc cagacgcagc gtcatcggcc ccgcgtttgc
      961 caaccagcca tctttgacaa tccatttttc attgttcagc catagtggca acgccccact
     1021 aaatgcccca gacatagcaa actgtttcgg attaaccgcg ctaaccagtt cgctggatga
     1081 gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag
     1141 cacgttaccg cctaacacat cgacgctaac atcagtcaac agcaatggtt cttcttcagt
     1201 ccacggataa cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac
     1261 ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa
     1321 acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc
     1381 gcctttcaac acgccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc
     1441 aacctgagca tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg
     1501 ctggaataca gtcagtgatt gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc
     1561 ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag
     1621 gaaatacgtc ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact
     1681 gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg
     1741 ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt
     1801 catcgtaccg tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct
     1861 gtcatgccac tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactccc
     1921 ttttccccag tagcgccact gccagcggcc agcgtcaggg agaaaatcat tcgccagccc
     1981 atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa
     2041 acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc
     2101 gatgtccagc gaatcgatga ctcttccctt tgaacgaagt aacgcgcctg gctcaaaggt
     2161 cagcgttggg tcagacagac ttccacttag ctgcgcaggt aaacgggcat ataaaatgag
     2221 gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc tggctgttat ccatgcttaa
     2281 ttttcctggg ccaaaattaa gcaccgcgtt gcccttaccc gcttgcccct gggtcagcac
     2341 actcagtcgt ccgctgacca gagcgttctc aagccctgcc tgccagttgt cgactttgac
     2401 acccagtcgg ccactcaaag gaaaacctgc atacggccag ctccagcgac catcgcttac
     2461 ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg
     2521 tgccagcacg atcaattgcc cgctattttc ctgccagtcc agctcggcat ccaccagtga
     2581 cggttcctgc ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg
     2641 catgagcggc atagtaaatt cccccaccag tttaaccgac ggctgatttt caaacgcgac
     2701 gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt
     2761 ttcgccctga taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg
     2821 ccattcctgc cagggagaaa aaatcagttt atcaatattg atccaggtgt taggcagcat
     2881 ggactgccat tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt
     2941 cgccagacaa gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg
     3001 tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc
     3061 gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc
     3121 gagcggtagc cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcagcagcgt
     3181 caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat
     3241 gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg
     3301 tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa
     3361 tatcgccata gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt aacttaaatg
     3421 tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac
     3481 gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac
     3541 tttctgctga cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc
     3601 gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat
     3661 atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg
     3721 ctgaaagtag tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt
     3781 atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac
     3841 ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt
     3901 accggtaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg
     3961 gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg
     4021 ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac
     4081 taccatctgt tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat
     4141 accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa
     4201 attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa
     4261 tccaacgacg tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg
     4321 tttaccgggc accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg
     4381 ctgcaaaact taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact ggtttaatct
     4441 tgccgctccc ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct
     4501 ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag
     4561 ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg
     4621 ttacgccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg
     4681 tgacctgcga taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca
     4741 gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag
     4801 ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca
     4861 ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg
     4921 cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa tagctgccac
     4981 agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc
     5041 caatcgccgt accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat
     5101 ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg
     5161 cctgctgaac aggctcacta ctacacgccg acaacaataa agcggcacac cctacccaaa
     5221 acgctgctcg catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat
     5281 aggcagcaca gagggcgacg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt
     5341 ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac
     5401 acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaagattac tactgggcgc
     5461 gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga
     5521 cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc
     5581 tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa
     5641 gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc
     5701 taccgtgcat ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg
     5761 tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct
     5821 gcatgtagcg gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga
     5881 cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc
     5941 gcaagacttt gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca
     6001 tttctttgat ggtttccgta cgtcccacga aatcaataaa attgtcccgc tggccgatga
     6061 cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa
     6121 cccggaacat cccgtgatcc gcggtacgtc agccaatcct gacacttatt tccagtctcg
     6181 cgaagccacc aacccatggt acaacgcggt ctatgaccat gttgaacagg cgatgaatga
     6241 tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc
     6301 ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga
     6361 attgctaacc cgtggcgaaa aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc
     6421 cgccaaacac ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag
     6481 aaccaaagaa cccggtgccc aggcagaacc gctctatctg gatgtaatga ccgcactggc
     6541 agaagccttt aataatggcg agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg
     6601 tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc
     6661 ggctaaaccg aaagcgcgct ttacggttgg tatttacgat gatgtgacca atctgtcact
     6721 gccgttgccg gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct
     6781 tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac
     6841 gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt
     6901 ttctcacctt cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga
     6961 ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa
     7021 acctggcggc attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt
     7081 gccgcaggaa gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc
     7141 ggcgaaaatc gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc
     7201 ttttttccat ctgacacaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc
     7261 gattgccaaa agttacagta gcaaaggtca ggatctggtg gaacgcaact ggcaggctct
     7321 ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc
     7381 caatcgaccg ccagtggttt ccgatgccgc ccctgctttc gtgaaaaccg taaccgctgc
     7441 gatgctcgcc gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg
     7501 gccgatgggc accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa
     7561 agaggaactc tgtacccaat gtaaccactg cgtcgccgct tgcccacact cagctattcg
     7621 cgcaaaagtg gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga
     7681 tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggctc cggaagattg
     7741 caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat
     7801 taaagccatc aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga
     7861 tttcttcctc aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgcac
     7921 atcgcagctg attacaccgc tgtttgaata ttccggtgct tgctccggtt gtggcgagac
     7981 gccgtatatt aaattgctga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac
     8041 cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa
     8101 cggtcgtggg ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg
     8161 tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc
     8221 cgataaaatc ccggcggaat tactgacggc gttgaaatca gacgccacgc cagaggttcg
     8281 tcgtgaacag gttgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact
     8341 gctgcgtgac gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg
     8401 ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa
     8461 cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac
     8521 accgctgggt gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct
     8581 tggcgtcagt atgatgatgt acggtcatgt ttatgtggct cagatttctc tcggcgcgca
     8641 gctgaaccag acggtgaaag cgattcagga agcggaagcg tatccgggac catcgctgat
     8701 cattgcttat agcccgtgtg aagagcatgg ttacgatctg gcactcagcc atgaccagat
     8761 gcgccaactc acagctaccg gcttctggcc gctatatcgc ttcgatccgc gtcgtgccga
     8821 tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac
     8881 gttacttcac gagcaacgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca
     8941 gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc
     9001 cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta
     9061 aaaaaaagcc cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa
     9121 tgcaatggcc caacagaaaa taaaatctgt gacaataaag gcatataacc cgcgcagaat
     9181 aacgtataga aaatta
//
LOCUS       DAZGXS010000158         9160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGXS010000158 DAZGXS010000000
VERSION     DAZGXS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            127..489
                     /locus_tag="ACHKG5_004128"
     CDS             127..489
                     /locus_tag="ACHKG5_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870122.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(668..1126)
                     /gene="ybbJ"
                     /locus_tag="ACHKG5_004129"
     CDS             complement(668..1126)
                     /gene="ybbJ"
                     /locus_tag="ACHKG5_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0870123.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1123..2040)
                     /gene="qmcA"
                     /locus_tag="ACHKG5_004130"
     CDS             complement(1123..2040)
                     /gene="qmcA"
                     /locus_tag="ACHKG5_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0870124.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2186..2863
                     /gene="fetA"
                     /locus_tag="ACHKG5_004131"
     CDS             2186..2863
                     /gene="fetA"
                     /locus_tag="ACHKG5_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0870125.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2850..3629
                     /gene="fetB"
                     /locus_tag="ACHKG5_004132"
     CDS             2850..3629
                     /gene="fetB"
                     /locus_tag="ACHKG5_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0870126.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3692..4546)
                     /gene="cnoX"
                     /locus_tag="ACHKG5_004133"
     CDS             complement(3692..4546)
                     /gene="cnoX"
                     /locus_tag="ACHKG5_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0870127.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4607..5416)
                     /gene="ybbO"
                     /locus_tag="ACHKG5_004134"
     CDS             complement(4607..5416)
                     /gene="ybbO"
                     /locus_tag="ACHKG5_004134"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0870128.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5406..6029)
                     /gene="tesA"
                     /locus_tag="ACHKG5_004135"
     CDS             complement(5406..6029)
                     /gene="tesA"
                     /locus_tag="ACHKG5_004135"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0870129.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6000..6686
                     /gene="ybbA"
                     /locus_tag="ACHKG5_004136"
     CDS             6000..6686
                     /gene="ybbA"
                     /locus_tag="ACHKG5_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0870130.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6683..9097
                     /gene="ybbP"
                     /locus_tag="ACHKG5_004137"
     CDS             6683..9097
                     /gene="ybbP"
                     /locus_tag="ACHKG5_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0870131.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2082 a         2364 c         2528 g         2186 t
ORIGIN      
        1 tgttttgata agcgaaataa attctgtttc tggactaaac gtgaagcggt tcctgatgtg
       61 tgctaaaatg cgtttctttg ataaatctca atttttaaga ggtttacgcc aggcaggaaa
      121 atttaaatga atgaatttga gaagattttt aatgaaatga atctggaccg ggcattgcta
      181 ccgatccttt ttcgttcgaa ccggtcgaca gtgtggaagt atttaagcgg cgatagtacg
      241 gctcctgcct ctgccatgtc gctgattatg ctgttgcagt tgatacagaa acgaaacccc
      301 gatctgttag ccgaatggtt aacgctttct gatttcacca ttccgccaga agtttacctg
      361 gaccagcctg attactggaa agggtgggtg tatacgcaac acaaggtcaa taaaaacgtc
      421 ctggaatatc taaaaaaaca ctatccggat gaagaccaaa aaagtatgag taaaggccgg
      481 gaagagtaaa ttgccaatgc cagttaacat ttaacatggc ggaggcggcc tacggttttg
      541 gcgtgatatg acgcgcccgc gtcgcatcag gcgtcggtgc acgactgccg gatgcggcgt
      601 aaacgcctta tccggcctac gattcgggcg cagacttccg ggcatgataa gacgcgtcag
      661 cgtcgcatca ggcgatgacc gcacggatga tcagcgttat cccttctatc gcaatgactt
      721 caacatgcgt acctgcgccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga
      781 cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca
      841 gctgctgccc gcgctggttt aaatgactgt cgctgtgctt ttgttcgcgc acccgccgcg
      901 acaaccattt ccaccacagc caggcggcga gcagcgtcag gacggcaaac atcacccctt
      961 gccactccca acccagcggc accagccaga ccaccaggcc agtaatcact gctgccacgc
     1021 cactccacaa caaataacca tttccgccca gcatctcggc tgccagcagc aaaccgccga
     1081 gactgagcca gaaaatatgt ggatgaacga ctattaactc catcatggct gagtccgctt
     1141 gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc
     1201 ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc
     1261 ggtgtatttc tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatggcttc
     1321 agacaccatt ttggtggcgc gggcttctgc ttcagcggaa cgttcacgcg cttcagcctg
     1381 taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc
     1441 ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt
     1501 acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc
     1561 gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcctcat cgacaatacg
     1621 cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc
     1681 caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac
     1741 ttcataagcc gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac
     1801 gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat
     1861 attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa
     1921 cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt
     1981 gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat
     2041 cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat
     2101 tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag
     2161 taaaaatcag aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa
     2221 cgtaggatat ctggcgggtg atgcgaagat tcttaataac atcaattttt cgctgcgtgc
     2281 tggcgaattt aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat
     2341 agttgcttca ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag
     2401 cacactaaag ccagaaatct accgtcagca agtctcttac tgcgcccaga cgccgacgct
     2461 gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgta accagcagcc
     2521 tgacccggcc atttttctcg attttcttga acgcttcgcc ttgccggaca gcattttgac
     2581 gaagaatatc gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct
     2641 gcaatttatg ccgaaggttt tattgctgga tgaaatcacc agtgcgctgg atgaaagtaa
     2701 taaacataac gttaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct
     2761 gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc
     2821 gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat
     2881 tagcactggc attaatgctg gtagtggtgg caatcttaat tagccataaa gaaaaactgg
     2941 cgctggagaa agatattctc tggagcgtcg ggcgcgcgat aattcagctg attattgttg
     3001 gctatgtgct gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt
     3061 tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag
     3121 cttttatctc atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga
     3181 ttctctcagg gtcgattgaa tttatcccga tgcaggtgat ccctatcgcc gggatgattg
     3241 ccggtaacgc catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta
     3301 gcgaacagca acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag
     3361 cgatattgat tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa
     3421 cggttggctt agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc
     3481 cggtgaaggc gattaaatat cagattatgg tgacctttat gctgctctca accgccagct
     3541 tgtcgaccat aattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt
     3601 tggtggtgac gcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg
     3661 cgttgtggtt catgtgccga tcaacggaat atcaatacaa caatgcatac agctggcggc
     3721 gatacttcga cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt
     3781 tacgcgtctg accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca
     3841 gcgcctcttc attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg
     3901 catcttctgg attctccgcc acctgctgtt gcaactgttg aatttccggc gtatcagccg
     3961 cctgcttcag cagttcgatt tgcgccacca gcccctggta gcgggtgtcc tgatcctgca
     4021 acggaatggt tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt
     4081 ctgccagcag caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca
     4141 atggcagggc atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca
     4201 gctcttcttc gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg
     4261 gcccctggaa gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc
     4321 gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa
     4381 attgcccgtt gtactgcgcc gcaaggcttt ccagaattgg ggttaactgc aaacagtgct
     4441 ggctacgttc agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct
     4501 gttgcaggtt agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg
     4561 ttgtcgatat tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata
     4621 ttttgtccat cacgcgcccc ggcagcaggc gcttaagcac cattaccgcc caggtcacca
     4681 gcgttaccgg atagcgcatc ttcggcttct cgctaataaa agcatggcgt actttgtcca
     4741 ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat
     4801 cactttgcgt ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca
     4861 ggctgacttt aattccgctg cggcgcagct ccatgcgcag cgcgtctgac cacgcctcca
     4921 gcgcatattt actggccgcg taagcgccac gacccggtgt ggagattaat cccatcaccg
     4981 atgaggtcat cacaatacgc ccttcaccgt gcggcaacat cgcgggtaac aggcgcatgg
     5041 tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga
     5101 tggtggaaag ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat
     5161 tatcggtcag ggcgatcacc tcgtcggctg cgcgatcaac actttctggt gaatccagat
     5221 cgatcaacac gccggtaaat cccatgctgt tcatgcgctc aacatcatcc ggtttccggc
     5281 aacctgccag cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc
     5341 cactggaaca tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg
     5401 ttgctttatg agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata
     5461 aacggctggg cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg
     5521 tagacctctt ccattaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg
     5581 taaatggcgc taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc
     5641 attaacaatg gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc
     5701 tcggtttgct gtggctgaaa accacgcaaa ccgtcattgc cgcccagttc aaccagcacc
     5761 caacgcggct gatgctgttt cagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg
     5821 tcgccgctga tgctggcatt gactaccgac gttttactct gccacttatc attcaacaag
     5881 gcaggccagg ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc
     5941 aataacgtgt ccgctgcggc agcacggaag gttaacagga ccagaaacag gaagggcaaa
     6001 tgccagcgga aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc
     6061 atgaactctc catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac
     6121 tggtgggcga gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggctggatg
     6181 acggcagcag tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag
     6241 cgcgggcaaa gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc
     6301 ctacccttaa cgcgctggaa aacgtcgagc ttccggcgct gctgcgcggt gagagtagcg
     6361 cggaaagtcg taacggagcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg
     6421 atcatcttcc ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct
     6481 ttaacggtcg acctgatgtg ctgtttgccg acgaacccac cggtaacctt gaccgccaga
     6541 cgggcgataa aattgccgac ctgctgtttt ccctcaaccg tgagcatggc accacgttga
     6601 ttatggtgac ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga
     6661 acgggcagtt gcaggaggaa gcatgattgc acgctggttc tggcgcgaat ggcgctcgcc
     6721 gtcgctatta attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg
     6781 caatatcagc gatcgcatgg agaagggctt aagccaacaa agccgggagt ttatggcggg
     6841 cgatcgggcg ttgcgcagtt cgcgcgaagt gccgcaagcg tggctggagg aagcgcaaaa
     6901 gcgcggcctg aaagtcggca agcagctgac tttcgccacc atgacatttg caggcgacac
     6961 accgcagctg gcgaacgtta aagcggtgga tgatatctac ccgatgtatg gcgatctgca
     7021 aaccaatccc cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat
     7081 ggcactgctt aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat
     7141 tgccggagaa gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg
     7201 tctgatgatg aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt
     7261 cacctggcgc tataaattcg gcggcagcga gaaccagctc gacagctatg agaaatggtt
     7321 gttacctcag cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct
     7381 ggggcgatcg atggaacgct cgcaacagtt cctgctgctt tcggcacttc tgaccttgct
     7441 gctggcagtg gcagcagtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct
     7501 ggtggcgatc ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg
     7561 tcagtggttg atggtgctga cgctttcagc cgttaccggt ggggcgatag gcctgttgtt
     7621 cgaaaacgtg ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag
     7681 cctctggcca tggctatggg cgctgggcac catgacggtc atctcgctgc tggtggggct
     7741 acgaccatac cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt
     7801 tgcgaacgtc tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct
     7861 gctcgccgga ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt
     7921 actggctttg ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac
     7981 gctgaaatcg ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac
     8041 gttaagccag ctttcggcat tttcgctctc ctttatgctg ctggcactgc tactggtgct
     8101 gcgtggcgat ctgctcgacc gctggcagca gcagttaccg ccagaaagcc cgaactactt
     8161 tttaatcaac atcgccacag aacaggttac gccgctaaaa gcgttcctcg cggaacatca
     8221 gatagtcccg gaatcgtttt atccggtggt gcgagcgcgg ctgacggcga ttaacggcaa
     8281 gccgacagaa ggcaatgaag atgaggcgct caaccgcgaa ctcaatctta cctggcaaaa
     8341 tacgcggccc gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ctgatgaagt
     8401 ttcgatggaa gaggggctgg cgaaacgctt aaacgttacc ctcggcgata ccgtgacttt
     8461 tatgggcgat acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga
     8521 aagtctgcgg cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca
     8581 gagctggctt accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg
     8641 ccagttccct accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt
     8701 gctggagcag gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat
     8761 gttgctgttg ctggcacagg tgcaggtggg aatgcgtcag cgtcatcagg agctggtggt
     8821 gtggcgcaca ctcggtgcgg ggaaaaaact gctgcgtacc acgttgtggt gtgagttcgc
     8881 catgcttggg tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt
     8941 gttgcaggcg aaagtgtttg attttccgtg ggagccagac tggcgattgt ggattgttct
     9001 gccgtgcagc ggtgcgctgc tgctgtcgct tttcggcggc tggctgggcg cgagactggt
     9061 taagggtaag gcgctgttca ggcagtttgc ggggtgatga gagtaaataa ggggcgttct
     9121 ggttgaatgg aacgccttgt ttaaattaat tctacaatat
//
LOCUS       DAZGXS010000159         8892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGXS010000159 DAZGXS010000000
VERSION     DAZGXS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..4229
                     /locus_tag="ACHKG5_004138"
     CDS             <1..4229
                     /locus_tag="ACHKG5_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0870132.1"
                     /translation="KAEIIGNGVDETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQ
                     SKSNTNDSGVAEVTFKGTVLGVHTAEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQ
                     VVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQANGEAHV
                     TLKGKKAGTHTVTATLSNNNTSDSQPVTFVADKTSALVVLQISKNEITGNGVDSATLT
                     ATVKDQFDNEVNNLPVTFSTASSGLTLTPGESNTNESGIAQATLAGVAFGEQTVTASL
                     ANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVK
                     GVTVNFTSNAATAEMTNGGQAVTNEQGKATVTYTNTRSSIESGARPDTVEASLENGSS
                     TLSTSINVNADASTAHLTLLQALFDTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSP
                     SEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQVTATLENGDSMQQTVTYVPNVANAE
                     ISLAASKDPVIANNNDLTTLTATVADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVV
                     TDTEGKAKVTLKGTKAGAHTVTASMAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIAN
                     NVGMTRLQATVTDGNGNPLANEAVTFTLPADVSASFTLGQGGSAITDINGKAEVTLSG
                     TKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLASLTSVYSFVVSTTEGATMTASVT
                     DANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEILVTSTEVGLKTVSASLADKPTE
                     VISRLLNAKADINSATITSLEIPEGQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEP
                     PEHMTISQNIVSTDTHGIAEVTMTPERNGSYMVKASLANGSSYEKDLVVIDQKLTLSA
                     SSPLIGVNSPTGATLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPV
                     VLTSNKVGTYTVTASFHNGVTIQTQTIVKVTGNSSTAHVASFIADPSTIAATNSDLST
                     LKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVRGAITGSVTVSA
                     VTTAGGMQTVDITLVAGPADASQSVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVS
                     EGLEFVQSGTNAPYVQVSAIDYSKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIQFTRAEDKIMSGTVLVNGANLPTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSS
                     SASWVDVDATGKVTFKNVGSKWERITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSL
                     AENFCSSNGYTLPLGDHLNHSRSRGIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANS
                     NEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(4491..5543)
                     /locus_tag="ACHKG5_004139"
     CDS             complement(4491..5543)
                     /locus_tag="ACHKG5_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP0870133.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            5857..7173
                     /gene="shiA"
                     /locus_tag="ACHKG5_004140"
     CDS             5857..7173
                     /gene="shiA"
                     /locus_tag="ACHKG5_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP0870134.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            7275..8729
                     /gene="amn"
                     /locus_tag="ACHKG5_004141"
     CDS             7275..8729
                     /gene="amn"
                     /locus_tag="ACHKG5_004141"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0870135.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTQFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2374 a         2125 c         2108 g         2285 t
ORIGIN      
        1 cgaaagcgga aatcattggg aatggcgtgg atgagacgac tctgacggca acagtgaaag
       61 atccttttga taacgcagta aaagatctac aggtcacctt cagtaccaac cccgcagata
      121 ctcaacttag tcagagcaaa agcaatacta acgacagtgg tgtggccgaa gttaccttta
      181 agggcacggt tttgggtgtt catacagccg aagccacact gcctaacggc aacaacgata
      241 cgaagatagt caatattgcg cccgatgcca gcaacgcgca ggttacgctg aacatccctg
      301 ctcaacaggt ggtgacgaat aacagcgaca gcgtgcagct gacggcgacg gtgaaagatc
      361 cgtcgaatca tccggtggcg ggaataacgg tgaacttcac catgccacag gacgtggcgg
      421 caaactttac cctcgaaaat aacggtattg ccatcaccca ggccaatggg gaagcgcatg
      481 tcacgctcaa aggtaaaaaa gcgggtacgc atacggttac cgcaacgctg agtaataaca
      541 ataccagtga ttcacagccg gtaacgtttg tggcggacaa aacctcggct ctggttgttc
      601 ttcagatatc aaaaaatgag atcacaggta atggcgtcga tagcgcaacg ctaactgcaa
      661 cggtcaaaga tcagttcgac aatgaggtga acaatcttcc ggtaacattc agcacagctt
      721 cttcaggcct caccctgacc ccaggggaaa gtaataccaa tgagtctggc atcgcgcagg
      781 ccactctcgc aggcgttgcc tttggtgagc agacggtcac tgcatcactg gctaataatg
      841 gtgccagcga caacaaaact gtgcatttta ttggcgacac agcggcggca aaaattatcg
      901 agttgacgcc tgtcccagac agcataatcg caggtacccc gcagaacagc tccggcagcg
      961 tcatcaccgc cacagtcgtt gataataatg gctttccggt gaaaggtgtg actgtgaact
     1021 tcaccagcaa cgcagcgaca gccgaaatga cgaatggcgg tcaagccgtg acgaacgaac
     1081 agggtaaggc taccgtcact tataccaata cccgctcctc gatagaatca ggagcgagac
     1141 cggataccgt tgaggccagt ctggaaaatg gtagctccac gcttagcaca tcaattaatg
     1201 tcaacgctga tgcgtctacg gcacatctca ccttgctaca ggcacttttt gatacagtct
     1261 ccgcaggcga cactaccaat ctgtatattg aggtgaagga taattacggc aacggagtac
     1321 cccagcagga ggtaaccctc agcgtttcac caagtgaagg tgtgaccccc agtaataacg
     1381 ctatatatac gaccaatcac gacggcaatt tttacgcaag ctttaccgct acaaaagccg
     1441 gggtatacca agtgacggca accctcgaaa atggcgattc gatgcaacaa acagtgacct
     1501 atgtgccgaa cgtagcgaat gctgaaatct cgctggcagc ctcgaaggat ccggtaattg
     1561 ccaacaataa cgatctcacg acactaacag caacagtcgc tgatacagag ggcaatgcga
     1621 tagccaacag tgaggtaaca tttactctgc cggaagatgt gagggcgaac ttcacgctgg
     1681 gcgatggcgg taaagtggtt actgatactg aaggcaaagc gaaagtcacg ctgaaaggta
     1741 caaaagcagg cgctcatact gttacagcat cgatggctgg cggtaagagt gagcagttgg
     1801 tggtgaactt tattgcggat acactcactg cgcaggttaa tcttaacgtt accgaggaca
     1861 attttatcgc taataacgtc gggatgacca ggctgcaggc aacagtgact gatggaaacg
     1921 gcaacccgtt agccaatgag gcggtgacat tcacgctacc ggcagatgtg agcgcaagct
     1981 ttactctcgg acaaggcggt tccgccatta ctgacatcaa cggcaaggct gaagttacac
     2041 tgagcggtac aaaatccggc acctaccccg tgacagttag cgtgaacaat tatggtgtca
     2101 gtgatacgaa acaggtgact ttgattgccg atgctggtac cgcaaaacta gcctccttaa
     2161 cctctgtata ctcattcgtc gtcagcacga ccgagggcgc gaccatgact gcaagcgtca
     2221 ctgacgctaa cggcaacccg gtagaaggta taaaagttaa tttccgcgga acttccgtca
     2281 cgctaagcag caccagcgtt gaaacggatg atcggggttt cgctgaaatt cttgtgacaa
     2341 gcaccgaggt cggactgaaa acagtttcag cctctctggc agataaacct actgaagtca
     2401 tctcgcgatt actgaatgca aaagcagata ttaattctgc aacgattacc agtctggaga
     2461 tacctgaagg tcaggtcatg gtcgcacaag acgtagcagt taaagctcac gtcaacgacc
     2521 agtttggcaa tccgattctt aatgaatctg taacattcag tgcagaacca ccagagcaca
     2581 tgaccatcag ccaaaatatt gtctctactg atacgcatgg tatagccgag gtcactatga
     2641 cgcccgaaag aaacggttcg tatatggtga aagcatccct ggcgaatgga tcctcttatg
     2701 agaaggatct ggtggtaatc gatcaaaaac tgacactctc ggcgtccagc ccgcttatcg
     2761 gtgtcaattc cccaacaggt gcaactctga cggcaacgct aacttctgca aatggcactc
     2821 cagtggaggg tcaggtcatc aactttagcg taacgccaga aggtgcgacg ttaagtggcg
     2881 gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt tctgaccagc aataaagtcg
     2941 gtacatatac ggtgactgca tcgttccata acggcgtaac aatacagaca cagacaatcg
     3001 tgaaagtcac tggcaactca agcaccgccc atgttgctag ctttatcgct gatccatcga
     3061 ctatagccgc caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg
     3121 gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctct gccacattaa
     3181 cgtcattaac agcggtgaca gatcaaaacg gaatcgcgac aacaagcgtg agaggagcga
     3241 taacggggag cgtcacggta agcgcagtca cgaccgctgg tggaatgcaa acagtagata
     3301 taacgctggt ggcaggcccg gcagacgcct cgcagtccgt ccttaagaac aatcggtcat
     3361 cattgaaagg agactttacc gatagtgctg agctacatct tgttctgcac gatatatcag
     3421 gcaatccgat caaagtttct gaagggctgg aatttgtgca gtcaggtacc aacgcgccct
     3481 atgtgcaagt tagtgcaatt gactacagta aaaatttctc aggcgagtac aaagccactg
     3541 ttacaggcgg cggagagggt atcgcaacgc tgatccctgt attgaatggt gttcatcaag
     3601 cgggtctgag taccacaata caattcactc gcgcagaaga caaaataatg agcggtacag
     3661 tgttagtcaa tggtgctaac ctaccgacaa ctacattccc ttcgcagggg ttcactgggg
     3721 cgtattatca gttgaataat gacaactttg ccccaggaaa aacggcggct gattatgagt
     3781 tttcaagctc tgcctcctgg gttgatgttg atgctaccgg taaagtgaca tttaaaaatg
     3841 tcggcagcaa atgggagagg attacggcga cgccaaaaac aggcggccct agctatatat
     3901 acgaaatccg agtgaagagt tggtgggtga acgccggcga tgctttcatg atatacagcc
     3961 ttgctgaaaa tttttgcagt agcaatggct acacacttcc ccttggagac catttaaacc
     4021 atagtcgttc ccgaggcatc gggtcactgt acagtgaatg gggagatatg gggcattaca
     4081 cgactgaagc tggttttcat tcaaatatgt attggtcatc gagtcccgca aactcaaacg
     4141 aacaatacgt agtttccctg gcaacaggtg atcaaagcgt atttgaaaag cttgggtttg
     4201 cttatgcgac atgttataaa aacctctgat tttccgttat aatttcttaa agagtgctcc
     4261 attcggggca ctctttttta cattccctct atagagcttg cccttctctc acagaaaatg
     4321 aataaataaa aatgcgatac cgcaagaatc gcgttcgatg tgactatgat cgtatccggt
     4381 gagtacagct tttatccaaa ggctggtttt ctgtgatgat tgaccagcct cagagtattg
     4441 atgacaccac cgttcataac agatcggcat tactatgcat aagtactgtc tcatattctc
     4501 gccaaaacac tttttaatgc ctttgcgaat tcatccagac ttatattgtc aattttttgc
     4561 ccaacgacga ctgactccga aacatcggcc attgcgggcc atcgcgtttt gtaatcagca
     4621 taaattgcaa caacgggggt attataggca cttgccatat gtaatattga ggtgtcagga
     4681 gttattgcga tataagcatc ttttatgatt gctgctgaac gtttaatcga gggtgataag
     4741 gataaacggt agacattatt ataacaatcc actaataccc tggctttatc ttcccctttt
     4801 ggtccatgaa cgatcactat tggtatatct gtttcacttt gaattttttc tattagattt
     4861 tctgcaatcg atagtgaaaa tgtacgatcc tgcgagctac cttctaaatt aagcgcaatg
     4921 taagatccta acgatcgcat ttcctcgcgc acctcatcga gtacatcctc acttagtggc
     4981 aattcatata ttggcctgac tgctggaaaa cccgcctcac gcatcataaa tgcccatgtc
     5041 atgggtacgg gagcccggag tttctgatcc atccgggacg cgttcttaca cacaggggaa
     5101 tagcacttca tggttaaacc aacaacctga aaattcgttt ttgctttcaa ctgactgata
     5161 aataacatcg ttttcaggtt ctttttacgc atcgcctcta tacaaagatc cggcgtaccg
     5221 tattgctgtg ttatgttctt cgctaaatct tttatttctt ttaatgttgc gtgatcctgc
     5281 atagtcattg tgactaatgt taatttagtc tgttcaagtt tgagcgcatt aaagacttct
     5341 aaattaattg tcgacgtaac aattaataga tgcttaattt tatgcaattc aagcgcccga
     5401 ataacaggaa agatggccat agcatcgcca atctgatcgg gaatatggat gacaacaaag
     5461 tctgtttttt caatattgaa attataagct ttataatcgt agtaactaaa tgcaatacgt
     5521 ctcaacaatg atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc
     5581 agtctatcag aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc
     5641 tcgaacctgt ggggcctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat
     5701 cttccagcga atttttcatg ttgccgttaa tcagccataa aatcatttgc agatgcgctc
     5761 tgtcgaggta gtctcataag gttcgtttat agatcgacgc aatgtgagtt accttttcca
     5821 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
     5881 cgtcccgatg aaaggacggt ttcgttaagt cgcgcccgac gagctgcgtt aggcagtttc
     5941 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc cgcactggtg
     6001 tttaatcgcg agtttttccc gcaagtaagc ccggctatgg gaacgctcgc cgcatttgcc
     6061 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
     6121 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
     6181 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttactg
     6241 gtgacgctgc gtgccattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
     6301 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
     6361 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
     6421 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
     6481 gtactgggag cattgtgggt gcgcaatggc atggaagagt ccgcggaatt tgaacaacag
     6541 caatataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
     6601 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg agttgctgac aatgtacatc
     6661 gttaccgcct ttgcacttaa ttattcaact cagaatatgg ggttaccgcg cgaacttttc
     6721 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
     6781 cttgccgatc gttttggtcg ccgcagggtt tatatcacag gcgcgttgat cggaacgttg
     6841 agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc
     6901 tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt
     6961 accgaaatgt ttggtgccag ttatcgctat agtggtgctg gagtcggtta tcaggttgcc
     7021 agtgtggttg gcggtggatt tacacctttt attgccgctg cgctcatcac ttactttgcc
     7081 gggaactggc atagcgtcgc catttatttg ctggctggat gtctgatttc cgcaatgacc
     7141 gctttgttga tgaaagacaa tcaacgcgct tgatagtctg gcgaagatca tccgatcttc
     7201 gccttacact tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg
     7261 aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag gcactggata
     7321 aactcgacgc gctgtatgag caatctgtag tcgcattacg caacgccatt ggcaactata
     7381 ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat
     7441 ctctgaccgt aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc
     7501 gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt
     7561 atcttaatga acaacttacg ttgctgtatc aggattatgg cgcgcatatc tcggtgcaac
     7621 cctcacagca tgaaatccct tatccttatg tcatcgatgg ctctgaatta acacttgatc
     7681 gctcaatgag cgctgggtta actcgctatt tcccgacaac agaactggcg caaattggcg
     7741 atgaaactgc agacggcatt tatcatccaa ctcaattctc cccgctatcg cattttgatg
     7801 cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta taccggtacg ccagttgaac
     7861 attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt
     7921 ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg
     7981 ggaactggat caccgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga
     8041 aaaaacacca gatgccagca tggcatttaa ttaccgccga tggtcagggt attactctgg
     8101 tgaatattgg cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg gcagtgctac
     8161 gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagt caggccattg
     8221 gcgattatgt acttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc
     8281 cacccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat gacgccacca
     8341 agctggtgag cggcaggccc ggtgaggaag tcaaacagcg gctacgtact ggtactgtgg
     8401 taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact
     8461 taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt
     8521 tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat ggcgagatta
     8581 aacttcccgg tcaggctaac cgtttttatg aaggcgctat ttccgaacac ctacaaattg
     8641 gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac
     8701 gaacctttaa tgagccgccg ttccgataat aagttctgaa ccttcttatc aaaaatagat
     8761 gtgaaggatt aagtaagaca tctggcaaat agcccgcaat aaacgcgggc tgtttgcttc
     8821 cttgggcgga tacgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat
     8881 gctttcaaaa ca
//
LOCUS       DAZGXS010000160         8841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGXS010000160 DAZGXS010000000
VERSION     DAZGXS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1022)
                     /locus_tag="ACHKG5_004142"
     CDS             complement(<1..1022)
                     /locus_tag="ACHKG5_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0870136.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGL"
     gene            complement(1171..1737)
                     /locus_tag="ACHKG5_004143"
     CDS             complement(1171..1737)
                     /locus_tag="ACHKG5_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0870137.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            2215..3003
                     /gene="fabI"
                     /locus_tag="ACHKG5_004144"
     CDS             2215..3003
                     /gene="fabI"
                     /locus_tag="ACHKG5_004144"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0870138.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            3147..4274
                     /gene="yciW"
                     /locus_tag="ACHKG5_004145"
     CDS             3147..4274
                     /gene="yciW"
                     /locus_tag="ACHKG5_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0870139.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            4342..6276
                     /gene="rnb"
                     /locus_tag="ACHKG5_004146"
     CDS             4342..6276
                     /gene="rnb"
                     /locus_tag="ACHKG5_004146"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0870140.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(6395..6457)
                     /locus_tag="ACHKG5_004147"
                     /pseudo
     CDS             complement(6395..6457)
                     /locus_tag="ACHKG5_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6511..8496
                     /gene="pdeR"
                     /locus_tag="ACHKG5_004148"
     CDS             6511..8496
                     /gene="pdeR"
                     /locus_tag="ACHKG5_004148"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0870141.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(8478..8606)
                     /gene="ymiD"
                     /locus_tag="ACHKG5_004149"
     CDS             complement(8478..8606)
                     /gene="ymiD"
                     /locus_tag="ACHKG5_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0870142.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            8644..8817
                     /gene="yciZ"
                     /locus_tag="ACHKG5_004150"
     CDS             8644..8817
                     /gene="yciZ"
                     /locus_tag="ACHKG5_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0870143.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         2163 a         2236 c         2123 g         2319 t
ORIGIN      
        1 aggccgttta gcaccagcca tttatcacca tatgtttctc ccgtttcgag cgttcgctgc
       61 tctactttat tgtctttatt caccaccagc gcagttgcat tgcctttagc atcgcgcgtg
      121 acgccctgtt gcggcgcgag aatagcgtct tccatcacgc cttcatcgac acgagcgcga
      181 acaaacattc ccggtaataa ttgctgttgt ggattgggga aaatcgcccg taatgtcacc
      241 gaaccggtag actcatctac agcgacttcg gtgagttcca gacgcccttt ttcgctgtag
      301 gttgtgccat cttccagaat aagtgagacg ctcatggtgt cactgttggt cgccagtgac
      361 tgtttacgca gacgtaataa atcgacactg gagcgagtga ggtcgacata cattgtatcc
      421 agaccacgaa tagtcgttaa cgctgtatct tgcgacgcgg tcaccagtgc gccaggggtt
      481 accgacgaaa tgccaatgcg ccctgaaatc ggtgcggtta ccgtggtcca gtcaagatta
      541 atgcgcgcag tttccagtgc ggcttttttc gccgctacac tggctttatc ttgtgcacag
      601 gtagactgag catcatcagc atcctgttgt gaaacaccgt tctctttcac cagtcgggca
      661 taacgctgcg ctttctggca atcggctttt accagtgcct gtgcttgttg taatgctgcc
      721 cgagcttcat tccatgcagc ctgataactg gccgcatcaa tctggtagag cggctgtcca
      781 gccttgacca gatcaccttc cttaaataag cgtttctgga taattccccc aacctgcgga
      841 cgtacttcgg cactgagcgc agcactggtg cgtccggtta attcactgac taccgagacc
      901 ggttgagatt tgactgtaac aacgccgact tctgtttctg acggggatga attgttactt
      961 tgcgtattat cacaaccaga taagagcagc attgccacta cagatgttgc tatatacttc
     1021 attattttct ccagggtgag aaataccctc ctcattaaaa ttaatgagga tatgtcaggg
     1081 atattcagtt ataacgaacc cgcatcgcga aatgaatttc gcaatgaaat aagggggatt
     1141 aatatccccc gctattggtt aattctgttc ttatttattt atcaacatac taacaatatc
     1201 ctgatatatt tcctgcagat gttgtggatc gcttttttgc ggtgttaaac gacgataaac
     1261 cgtaccttcc atcatggtag caaacacttc aacgcagcaa cgaatgtgct cctcgctaag
     1321 atggggatgc attcttttca tatgtgcaca accgttagcg aacattctgg cgtcagcttc
     1381 ttcaagcatt gccataaccc gcgggttgcg tgtggcttct gcagccactt ccagcatcag
     1441 cgcgtcgtcg tcctcattta aggatttacg caaggcaagg acttccggga agtgatcggt
     1501 acgcgcgtca atgtccattt gggcaatacg aaagtcgatg atacggcgaa ccatctcttc
     1561 aataatggcg tctttgttgg cgaagtaacg atatatctga cccacgctaa gctgagcttc
     1621 gctggcaatt tgcgacatac tggcggcatg aaacccgcag cgacgaaaac accgacgggc
     1681 ggcgttaatg atttcatcct gacgctgttt gtggcgtatt tccagcttgc tggtcatttc
     1741 tgtctcctgg cctatcactt ggtgagaatg atcgttctca tttttgctga ttatacgttt
     1801 ctccaaagta atggaaagta tttttatctt attcatggtg catattctga taatgaagag
     1861 attgtggaga ttcagtgaag atagttgaca atattaagtt ggggtcaatt catcagaaag
     1921 gctgctactt gttgtcgcca gttctgtttt catcatcaaa cttatcggag ctaatacgaa
     1981 tagtttcata gtcagattct ataagatatt tccccaacct accccttgca ggcgtcaaga
     2041 gtagataacg caaaatgctc tgtaccgcag cttctctccg gtacagaaag cgcaactata
     2101 gccacccaca gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac
     2161 gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt
     2221 tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac
     2281 ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac
     2341 aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcaa
     2401 tgcgatgttg ccgaagatgc cagcatcgac accatgttcg ctgaactggg gaaagtttgg
     2461 ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt
     2521 gactatgtta acgccgttac tcgtgaaggc ttcaaaattg cccacgacat cagctcctac
     2581 agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg
     2641 accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca
     2701 aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg
     2761 cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg tatcaaagac
     2821 ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt
     2881 gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg tatctccggt
     2941 gaagtggtcc acgttgacgg cggtttcagc attgctgcaa tgaacgaact cgaactgaaa
     3001 taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct gttatacctt
     3061 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
     3121 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
     3181 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
     3241 ccgcaaaggt cagcgatccc tttctactcg acgtgatcct tgaaaaagaa acgctggccc
     3301 ccttcctttc atggctggac cctgcgcgtg ttcttgcagt ggagttgttc cctgaccagc
     3361 ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtcgacg gccctgacgg
     3421 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
     3481 tccccgggcc tgattccacc agggaaagta atcatcggtt ggcacaaatc acgcaatatg
     3541 cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg
     3601 acgtcggtct tactgcctgg gactgtgtga tcattaacca aatcattggt tttattggct
     3661 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
     3721 ccgggctgga gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcattacgct
     3781 ggcgaagcag ctatgaggtg gaaaaactac ctgaagaaca cacaaaaagt tcaactgcag
     3841 aactttgcca actggccgaa atactctctc tccaccctat ttcactttcc cttctcgaaa
     3901 ggttgttaaa cagcacacgg gttaatacac agccggataa tcagcttgcg gcgttgttat
     3961 gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg tatggattca tcaaatgaat
     4021 ataaaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc
     4081 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
     4141 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
     4201 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
     4261 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
     4321 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
     4381 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggtttt
     4441 ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
     4501 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
     4561 gagccagaag aactggttga accgtttctg actcgtttcg tgggtaaagt tcagggcaaa
     4621 aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat tccttgccgc
     4681 gcagcccgtg gtctgaacca cgagtttaaa gaaggcgact gggcagttgc cgaaatgcgc
     4741 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
     4801 ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
     4861 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtaa agatctgacc
     4921 gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga cgcccttttc
     4981 gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
     5041 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
     5101 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
     5161 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
     5221 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
     5281 gaccaggttt ctgactggct ggagaatacc ggtgactggc agcctgaaag tgaagcaatt
     5341 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
     5401 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
     5461 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
     5521 attgccgcta acatttgtgc agctcgcgta ctgcgcgata agctcggttt tggtatctat
     5581 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
     5641 cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg caaactgcgt
     5701 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
     5761 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc
     5821 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
     5881 gttatcaaag gcgaaactgc gacgcgtcca caggatgaaa tcactgtcca aatggccgag
     5941 cgtcgccgtc tcaatcggat ggcagaacgt gatgttggtg actggttata cgcacgcttc
     6001 ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg aaattgtcga tatcagccgt
     6061 ggcggcatgc gtgttcgttt ggttgataac ggcgctatcg cctttattcc tgcacctttc
     6121 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcaccgt acaaattaaa
     6181 ggtgaaacgg tttataaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
     6241 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctta
     6301 tgaatgggcc gtttatttcc ccgctctacc ttcatcatat cccggcagtt ttttaactga
     6361 ctttcgtttg aaaactggcg tttttccaaa actagattac aaataaatat ataaaaaaaa
     6421 caatgtgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta tttaaccgcg
     6481 gactccgctg ttaaccggag gatatgcatc atgaaaaccg ttagggagtc cacaacgttg
     6541 tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag cgatgttttg
     6601 cgcttttcta ccaccgaaac cacagaacct gaacgtacgt tgcagttatc tgccgaacag
     6661 gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag tctgaccgtc
     6721 gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg ggaatgggct
     6781 ggcaacgcgt ctgcatggca tgacacacca gcagttgctc gtgatttatc acacggactt
     6841 tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct cgacagccgg
     6901 gggaatatcc aacgcttcaa tcggttatgt gaagattaca cagggttgaa cgaacacgac
     6961 gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc ggcatccagg
     7021 cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact gtggatacca
     7081 acacgtaaag gccagcggct gtttctgttt cgcaataaat ttgtccacag cggcagtggc
     7141 aaaaacgaga tttttttaat ctgttccggc accgacatta ccgaagagcg ccgcgctcag
     7201 gaacgactgc gtattctggc aaataccgac agtatcaccg gactgccgaa tcgtaacgca
     7261 atgcaggagt taatcgatca cgctattaat caggcagata acaataaagt tggggtcgtg
     7321 tatcttgatt tggataattt taaaaaggtc aacgacgcct atgggcattt atttggtgac
     7381 cagttattac gcgacgtgtc attggctatt ttaagctgtc tcgaacatga ccaggtgttg
     7441 gcgcgtccag gtggcgatga gtttctggta ctggcatcca acacctcaca aagcgcgctg
     7501 gaagcaatgg catcacgaat tttgacccgc ttacggctcc cctttcgcat tggtttaatt
     7561 gaagtttata ccagctgttc agtaggtatt gcactctctc ccgaacatgg ttcggacagc
     7621 gcggctatta ttcgtcacgc cgacacagca atgtacacag caaaggaagg cggacgagga
     7681 caattttgcg tttttacccc agaaatgaat caacgggtat ttgaatatct ctggctggat
     7741 accaacttgc gtaaagcact ggaaaacgat cagttggtta ttcactatca acccaaaatc
     7801 acctggcgtg gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt
     7861 gggttgattc caccgttgga cttcatttcc tacgcagaag agtcagggct aattgtgcct
     7921 ttaggccgtt gggtgattct cgatgtcgta cgccaggtgg cgaagtggcg ggataaaggc
     7981 attaacctgc gagtggcggt aaacatttct gcacgtcagc tcgccgatca aaccattttc
     8041 accgccctga aacaggttct ccaggaactc aattttgaat actgccctat tgatgttgaa
     8101 ttgacagaga gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt
     8161 agccaactag gtgcgcaagt gcatctggat gattttggta ccgcctactc ttcactttcg
     8221 caactggcgc gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt
     8281 cacaaacaac ctgtctcgca gtcactggtt cgggcaatcg tcgctgtggc ccaggcattg
     8341 aatcttcagg tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag
     8401 aatgggatca atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc
     8461 gaacgctggt ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg
     8521 ctatattccg tcattatata tcgtattccc ggatgataat tagccgttgc tgttaaacac
     8581 ttctctttta gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc
     8641 ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc
     8701 cttgtggcag acgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc
     8761 ttgcgccagg ttgctgaatc tacgccagac atcccgaagg caccgtggga gatttaacaa
     8821 tcgtgccttc aggttttcct a
//
LOCUS       DAZGXS010000161         8796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGXS010000161 DAZGXS010000000
VERSION     DAZGXS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..819
                     /locus_tag="ACHKG5_004151"
     CDS             <1..819
                     /locus_tag="ACHKG5_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP0870144.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIILKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(869..1111)
                     /locus_tag="ACHKG5_004152"
     CDS             complement(869..1111)
                     /locus_tag="ACHKG5_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0870145.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            1243..2526
                     /gene="ydfJ"
                     /locus_tag="ACHKG5_004153"
                     /pseudo
     CDS             1243..2526
                     /gene="ydfJ"
                     /locus_tag="ACHKG5_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2615..4075
                     /gene="ydfI"
                     /locus_tag="ACHKG5_004154"
     CDS             2615..4075
                     /gene="ydfI"
                     /locus_tag="ACHKG5_004154"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0870146.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(4111..4314)
                     /gene="ydfZ"
                     /locus_tag="ACHKG5_004155"
     CDS             complement(4111..4314)
                     /gene="ydfZ"
                     /locus_tag="ACHKG5_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0870147.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4491..5177)
                     /gene="rspR"
                     /locus_tag="ACHKG5_004156"
                     /gene_synonym="ydfH"
     CDS             complement(4491..5177)
                     /gene="rspR"
                     /locus_tag="ACHKG5_004156"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0870148.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(5266..6012)
                     /gene="ydfG"
                     /locus_tag="ACHKG5_004157"
     CDS             complement(5266..6012)
                     /gene="ydfG"
                     /locus_tag="ACHKG5_004157"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0870149.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            6149..8194
                     /gene="dcp"
                     /locus_tag="ACHKG5_004158"
     CDS             6149..8194
                     /gene="dcp"
                     /locus_tag="ACHKG5_004158"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0870150.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(8239..8757)
                     /gene="ydeJ"
                     /locus_tag="ACHKG5_004159"
     CDS             complement(8239..8757)
                     /gene="ydeJ"
                     /locus_tag="ACHKG5_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP0870151.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2203 a         2085 c         2245 g         2263 t
ORIGIN      
        1 gatgtctcac cggcgctggt atcgaaggtc accgatgccg ttatggagca ggtagtcgaa
       61 tggcaaaacc ggccactgga tgcggtctat cccattgttt atcttgactg tattatcctg
      121 aaggttcggc aggacagtcg cgtaatcaat aaatccgtat tcctcgcact gggtatcaat
      181 atcgaaggcc agaaagagtt actgggtatg tggctggccg aaaacgaggg agcgaagttc
      241 tggctcaatg tgctgactga actaaaaaac cgcggcctga acgatattct catagcctgt
      301 gtcgacgggc tgaaaggctt cccggacgct atcaacacgg tgtatccgga agcccgcatc
      361 cagctgtgca tcgtgcatat ggtgcgcaac agcctgcggt tcgtttcctg gaaggactac
      421 aaagccgtca cccgcgacct gaaagccatc taccaggccc ccacagaaga agcaggccag
      481 caggcgctgg aagcgttcgc cagcgcctgg gacagtcgct acccgcagat aagccggagc
      541 tggcaggcaa actggaccaa cctggcgatg ttcttcgctt acccggcaga tatccgcaaa
      601 gtcatctaca caaccaacgc catcgagtcg ctgaacagcg taatccggca tgccatcaaa
      661 aaacgcaagg tgttcccgac ggatgactca gtgaaaaagg tcgtgtggct ggcaatccag
      721 gcggcttcac agaaatggac gatgccactg cgggactggc gtatggcaat gagccgcttt
      781 attatcgagt tcggtgaccg cctggacggt cacttctgag aaaaggcatt tacacagaat
      841 cgtgtacagg gtcgatttta ctcgttaatt agcgaacaaa ccactcgtca gcagactccc
      901 aggtatcttt cagcgtctcc tgaacaaaag tttttgcaga atctttatcg gcggtgcgag
      961 taactgaaag gccatcgttg ctggtggatt tgatgatcac ttctacatcg tcataacgtt
     1021 tactgacacg tcgcagcatt tcttgttgca gggcaggaac agaacctttt ggcattttgt
     1081 tgattttggc tgcggggtat ctgtggggca tatatgggac ataaaaaggc ctcaaataaa
     1141 cagcaaaatc gagattaggg atttttagaa aaaatacaac catctgaaaa aacttagaaa
     1201 acacccaaaa acccacagaa tagtaaaaaa catctatgaa ttatggattt ccagttatat
     1261 tcgctcggcg cagcgttagt gtttcatgaa atattttttc ctgaatcatc aacggcaatg
     1321 gcgttaattc tggcaatggg aacctacggt gcaggttatg tggcgcgtat tgtcggagca
     1381 tttattttcg gcaaaatggg cgacagaata gggcgtaaaa aagtgctctt tattaccatc
     1441 accatgatgg ggatctgtac caccttaatt ggagtgttac cgacctatgc acagattggt
     1501 gtttttgccc ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg
     1561 gaaatttccg gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt
     1621 atctcctcat ttgtggctat gggaactaac tgcggaacct tgagcgcaac ggcaatctgg
     1681 gcctttatgt tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg
     1741 ttcctggcga gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa
     1801 agcccggtct ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct
     1861 ggtagcatat tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggca
     1921 ggtaactccg ggttaattca gactttcctt gcaggctatt tagtacagac gttattgttt
     1981 aacaaagcaa ttccaacaga tgcattgatg atcagttcga ttcccggctt tatgaccatt
     2041 ccgttccttg gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat
     2101 acctccgcga ttgtgctggc atggccaatg ctttctatca tcgtagataa aagctatgcc
     2161 ccgagcacca ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt
     2221 gctctggaaa acattaccat ggcagaaatg ttcggctgta aaaaccgctt tacccggatg
     2281 gctatttcta aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt
     2341 attttctgca ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca
     2401 gtgattggtt taatctctgc gctgaaaatg ccagaagtga aagaccgtga tttaagtgcg
     2461 ctggaagacg ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt
     2521 ctgtaaatcc tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca
     2581 tgtagcttgc catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa
     2641 agcgacactc cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt
     2701 tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt
     2761 cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt
     2821 acatcagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg
     2881 cgtcgttggc gtcgttaaaa aagccttgca cgtacagata gatggcttag aaaccgtgtt
     2941 ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta
     3001 tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg ctgccgacgt
     3061 gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg
     3121 taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg
     3181 tcatgttatg cgtgacgttg tcacttccta cgcacaagcc gttgatgtaa aactagcaca
     3241 atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt
     3301 gacagaggat acgctggcga aaatcgaaca gcttaccggt gtgcgcgatc ctgctggcgt
     3361 tgcctgtgaa cctttccgcc agtgggtaat cgaagataac tttgttgccg gacgtccgga
     3421 atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt
     3481 gcgcatgctc aacggcagtc attcattcct ggcgtatctg ggttatcttg ccggatatca
     3541 gcacattaat gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg ccttgatgtt
     3601 gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg
     3661 attaattgca cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga
     3721 tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga
     3781 cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt
     3841 tgatgaacag ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc
     3901 tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcgat
     3961 ttttggtggt gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt
     4021 gtctttatta gcgcatggtg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa
     4081 gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa tcaggtccag
     4141 tggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc
     4201 agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac
     4261 cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt
     4321 tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt
     4381 cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc
     4441 ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa ttactcttcg
     4501 ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa
     4561 tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaatata
     4621 tcaagatgct ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc
     4681 acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca
     4741 atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg
     4801 cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg
     4861 gtaatcatac tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg
     4921 ccgttgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt
     4981 tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca
     5041 ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg
     5101 acaatgtcgc gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt
     5161 tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact
     5221 tttttgtgac aagcaataac ccggcagtta caccgggtat aaaaattact gacggtggac
     5281 attcagcccg gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg
     5341 agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg ttaatgcaac
     5401 ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa
     5461 ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag
     5521 gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt
     5581 accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat gaccatgatt
     5641 acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt tgttgttggt
     5701 atcaatcatc gtttcccagt cttcaatgct ggctttatgc gcaggctcca tgcccaacgc
     5761 cagaccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa gcgatgccag
     5821 catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatgtaca gattatctcc
     5881 cagttcgtct tttaactcct gcaaccgttc ctggcgacgg ccagtggcga taactttatg
     5941 cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac
     6001 taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg
     6061 cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta
     6121 tggaacacct cgcgttcagg agaagaaaat gacaacaatt aatcctttcc ttgtgcaaag
     6181 cacactgccg tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc
     6241 attcgatgag ggaatacagc aaaagcgggc agaaattgct gccatcgcgc ttaacccgca
     6301 aacacctgat ttcaacaata ctattctggc actggaacaa agcggagaat tacttacccg
     6361 cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct
     6421 tgacgagcag ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga
     6481 attattcgcg cgggtagatg ctgtctggca acgccgtgaa tctctggggc ttgatagtga
     6541 atccatccgc ctggtggagg tgattcatca acgttttgtc ctagccggag ccaaacttgc
     6601 acaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgacac tgaccagtca
     6661 gtttaaccag cggttactgg cagcaaataa atccggcggt ctggttgtga acgatatcgc
     6721 gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa
     6781 aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc
     6841 cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga
     6901 aaaaaatgat gccaatgata cccgcgctat cattcaacgt ctggtggaga tccgtgcaca
     6961 acaggctaca ttactcggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc
     7021 aaaaacacct gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg
     7081 tgcgagcgat gaattagcct ccatacaggc ggttattgat aagcagcagg gcgggtttag
     7141 cgcgcagccg tgggactggg cattttatgc cgaacaggtc cggcgagaga aatttgatct
     7201 tgatgaggcg cagctcaagc catattttga attaaacacg gtgttgaatg aaggtgtatt
     7261 ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta
     7321 ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc tggcgttatt
     7381 ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt
     7441 tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa
     7501 acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtcataacct tattccatga
     7561 atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tctccggcac
     7621 caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca
     7681 tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga
     7741 actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact
     7801 gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca
     7861 ggatgtcgat gattttgaat tgcgggcgct ggtggcagaa aatatggatc ttcctgctat
     7921 accgccacgc tatcgcagca gttattttgc ccatattttt ggtggcggat atgccgcagg
     7981 ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga
     8041 gcagggcgga ttaacgcgtg aaaatgggct gcgttttcgc gaggcgatcc tttccagagg
     8101 taacagcgaa gatctggaac gcctgtatcg acaatggcgc ggtaaggctc ctcagattat
     8161 gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg tcctggagat
     8221 atcgccaggc cagcctggtt atagcagtaa ctgcagcagc tgggcgaggg caaacctcac
     8281 cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg
     8341 gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc
     8401 cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc
     8461 ggttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagattgctg
     8521 gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta
     8581 aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga
     8641 ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag
     8701 gttttcaata gtgtcggtat ctgcaagttg tacaatttta tttctattaa tgttcactgt
     8761 tttttgtcca cgcttttata tgtaaaggtg atttgc
//
LOCUS       DAZGXS010000162         8699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGXS010000162 DAZGXS010000000
VERSION     DAZGXS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..298)
                     /gene="gadE"
                     /locus_tag="ACHKG5_004160"
     CDS             complement(<1..298)
                     /gene="gadE"
                     /locus_tag="ACHKG5_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0870152.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLL"
     gene            complement(1097..1669)
                     /gene="hdeD"
                     /locus_tag="ACHKG5_004161"
     CDS             complement(1097..1669)
                     /gene="hdeD"
                     /locus_tag="ACHKG5_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0870153.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1924..2256
                     /gene="hdeA"
                     /locus_tag="ACHKG5_004162"
     CDS             1924..2256
                     /gene="hdeA"
                     /locus_tag="ACHKG5_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0870154.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            2372..2698
                     /gene="hdeB"
                     /locus_tag="ACHKG5_004163"
     CDS             2372..2698
                     /gene="hdeB"
                     /locus_tag="ACHKG5_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0870155.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2762..3409
                     /gene="yhiD"
                     /locus_tag="ACHKG5_004164"
     CDS             2762..3409
                     /gene="yhiD"
                     /locus_tag="ACHKG5_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0870156.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(3461..4231)
                     /locus_tag="ACHKG5_004165"
     CDS             complement(3461..4231)
                     /locus_tag="ACHKG5_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP0870157.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(4228..5220)
                     /gene="shuU"
                     /locus_tag="ACHKG5_004166"
                     /gene_synonym="chuU"
     CDS             complement(4228..5220)
                     /gene="shuU"
                     /locus_tag="ACHKG5_004166"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP0870158.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(5269..5892)
                     /gene="chuY"
                     /locus_tag="ACHKG5_004167"
     CDS             complement(5269..5892)
                     /gene="chuY"
                     /locus_tag="ACHKG5_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP0870159.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5892..6386)
                     /gene="hutX"
                     /locus_tag="ACHKG5_004168"
     CDS             complement(5892..6386)
                     /gene="hutX"
                     /locus_tag="ACHKG5_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP0870160.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(6399..7736)
                     /gene="hutW"
                     /locus_tag="ACHKG5_004169"
     CDS             complement(6399..7736)
                     /gene="hutW"
                     /locus_tag="ACHKG5_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP0870161.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(7756..8670)
                     /gene="shuT"
                     /locus_tag="ACHKG5_004170"
     CDS             complement(7756..8670)
                     /gene="shuT"
                     /locus_tag="ACHKG5_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP0870162.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         2344 a         2053 c         2057 g         2245 t
ORIGIN      
        1 caagaagatt tttgatactt tctttgcggc taacaaaata aataggcgct ttagctttta
       61 gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat ttctccagaa
      121 atttaatcgc ttcttcatca aggatatgat tgtgataggt atcaatgata accttgaagg
      181 gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt tcgtgattat
      241 ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa
      301 cttgctcctt agccgttatc gtttcgaaca tgtcatcctt ttcattattt acatccttgt
      361 ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tgcccttcgt gaactaatac
      421 gtctattaac actaatcaat acttcagatt tatccatcac gcactccata tattgatcct
      481 aagtaagatt cctggttgtt atcagcttgt aaatagtaaa caaaaagtta acgtttgcgg
      541 attactgccc aagaataagt tatcaataac cagtttgtga tctctgaaga atattactaa
      601 agttaaaaag taatccgatt taaatatcga gtctccttgt ttcgacttaa gctggcaatt
      661 ggattgccag ctttctttta acgcattgaa attacctgag taaataacgc attcatgtct
      721 tcaggttttt atatagcgtc tggagacatg gatatttatg caatgtaatc aacgccagtt
      781 gcaaaaaaat gaacccggat tacttattta accgcccata gcatcaacgc ttacggaact
      841 ctttgttcac tttcccacag cacttttaaa acattaaacc tgactacgga aaatatcagc
      901 catgataatg tttgaaatgg ctaatttgcc atggagtgaa aaaaattaga tgaaattcag
      961 taggttgaaa taatcactag caggtaatta tttcaatgat agtgcgcaat tgatctacaa
     1021 cactgcgtag cggagagagt attaatcgga tcatagtcac atcaagtgac tatgatccgg
     1081 gtgacaaccg ggataattat tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg
     1141 cggcgctaaa tatcagttca attcccacca gtgtggaaac cagcgttaca gacaccatcg
     1201 gcgttgcacc aaggaatatc caggcaatga cgatatccag cacaccaata acgagctgta
     1261 gccagctgcc tttcattgaa cgctgacgat accaactcat caggcgaata acccctgcaa
     1321 cacagaacaa accggcaata aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga
     1381 tgaagaaata gccgatcaat aaataggcga ctgcgacgag gaaaccggat aataccggcc
     1441 agaaattatg actgcggttg ctgaataatc caacaataag cgcaatgccc gaacagatta
     1501 ataatgcacc tactactgtg cttaaaatat cgccagaaac gaacgggaaa ctgatacaca
     1561 gcaacccgac gataaacagc agcacggcaa taaactggat tgctctgcga tgctttttca
     1621 gcatctccag atcaaacttc aaaattgttg ccttatctat atataacata gaaccaccct
     1681 ataaaattaa gaagaaaatc ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgc
     1741 agtcgattta ataaaaattt cctaattgca gtatctgatg catctgtaac tcattgtatt
     1801 gaaataaaaa tatctgattt tgatattttc catcaacatg acatatacag aaaaccaggt
     1861 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
     1921 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
     1981 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
     2041 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
     2101 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
     2161 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
     2221 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
     2281 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
     2341 tatataatct ctatattgaa tgggttacaa aataaatatt tcatctctcc gtaaagcgtt
     2401 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
     2461 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
     2521 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgatacagt
     2581 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
     2641 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
     2701 aggtacaagt aaaaaggagt ggcaagttga gccatcttgc tgctcctttt tgcattttta
     2761 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
     2821 catggaaagg caaatgcgcg gcaaaggagc agggttacgc actcatgtat taattggcat
     2881 gggaagcgca ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     2941 tcacgtcgga ctggacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     3001 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtttgacga cggcagcgga
     3061 tatctgggtg accgccgcca taggtatggt tattggcagt ggtatgtacg aactgggtat
     3121 ttatggttca gtgatgacct tgctggtgct ggaagtcttc catcaattaa ccttccgcct
     3181 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     3241 cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg aaaatgaaga
     3301 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagatctgtt
     3361 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtatagtt
     3421 atttattcgc aggagtacca gtgcggtact cctgttgctt ttaatgatcg aggacgatca
     3481 gtggtgaatg gttggcagga tctttaagta cggtaatatc cgcgccgtag agcattgtca
     3541 gcgcctgctg tgtcaatacg tcctgtggtt taccatttgc gataaccttt cctttttgca
     3601 ttaatacaac acggtctgca taatgcgcgg caagatttag atcgtgcaat atgcagcaga
     3661 cattgaactg ccgctcgtga actaactggc gtaataaacg aaacaagtgc tgctgatggt
     3721 gaatatccag cgccgaagtt ggttcatcaa gaaataacca tttcggtgag ggagttggtt
     3781 cccagagttg caccagcagg cgcgctaatt gcacgcgctg ttgttcacca ccggataatt
     3841 gccgatagtc gcggtttgct aacgcctgac agtcacataa cgccattatt tgtgctgttt
     3901 cattatcctg attaccagta cgatgcggat gccgacccat ctggataact tcttgtacgc
     3961 tgaatggaaa cgccatgtgg ctattttgac gcatcacggc ccggtgtttc gccagttcag
     4021 taatcgacca ttcatttaat ggcttattaa acaaccgaca ttcgccgcta tcaggttgaa
     4081 gatagccggt taactggcga agcagcgtag attttcccgc accattaggg ccgagaatgg
     4141 caacaatttc cccaccggga aaagtcagcg agacattatc agtcagacgc cgcccctgaa
     4201 ggctataaac cagattctgg gcgctgatca tgtgatcctc cgatttcgca gaatcaacca
     4261 cataaaataa gggccaccaa gcaggctggt taataatccc acgggcattt ctgctggctg
     4321 taccagcgtg cgggcaagcg tgtctgccat cagcaataaa caggctccgg cgagggcgga
     4381 acaagggatg agccagcggt gatttgctcc ggtagtcatg cgaatcagat gcgggatcac
     4441 caggccaata aagccgataa tgccgcttac cgatacggca gcaccaacga gcagtgagct
     4501 cactaatagt aattgctggc gtttacgctt aacgttcacg ccgaggtaat gggcttcttc
     4561 atcaccgagc tgcagtaaat tcagcgttcc ggcgagacaa gttgttgcga taatggccgg
     4621 aaggatgaat gaactggcaa ccaataacgt cggccattgc gcctgtccaa gattgcccat
     4681 catccacaat gtgagttgtc gtagctgctg ctcatcgcca atatagctca ggatgccgat
     4741 cgctgcgccg cacagggcat tgatggcgat accggcgagt aataattgca tcatgctgcc
     4801 atcgcgatgg cgctgcgtga tgagaaaaat gatggcgcag actactaaac ttccggcaat
     4861 ggcaaacacc atttgctcat agagcaccag caccactggg aaggagaagg gcagcacaat
     4921 tgcaacgcca accattaatg cagaaccgct actgacgccg agtaatcctg ggtcagccat
     4981 tgggttgcga aaaagcccct gcataattac ccctgcggtt gccaacgctg cacctaccag
     5041 caccgccagc aaaacccgtg gtagacgaat attgagccag atatcgcggt attcctcatc
     5101 aaaaagggca tcaaaactga tctttaacgc tccctgacta gcaccgaaca gcaccagggc
     5161 aagcaataat agtgatagcc ccataaagac cctggcggat gaaaatgaat ccttgagcac
     5221 gcaaaactcc gttacgacat tacggcgcgg acatgccgcg ccggggtatc acgccggttt
     5281 caggtccggt tcaatcagac tgtagacctg ctggttaagt gctggcgcat ttgccagttc
     5341 atggatatgt gcggcgacat cggcacggcg gacaaaacca tgacactcct gattttgtat
     5401 ccgttgtgct ttgcctgttg ctgcgccgtc aagcagacca ccagggcgga gaatggcgta
     5461 gtctagctgg ctggtttgca gccagctttc cgccagcgtt ttttcccgta ccgcctgacc
     5521 aaacgcagcc tttgcccgct ccgataaaaa cggccagcta tcgccgcatc ccaaagaggt
     5581 caccagaatc atgcgactga tgccggcctt ttcggcctca tcaatcactg ttctgtgcgc
     5641 cagataatcc tgagctcctc ccattgttga gatgataagc gcatccgggc cagctgcgcg
     5701 gcaagcagcc gcgactacgc tggcatcaca ggcgtcgcct gtaaaaacct gtacgccttg
     5761 ttgcgccagt ttcgtggcgg catcggcatg acgaatgaca gcaacgaccg gacgttgttc
     5821 cgccagcgcc agttcaagcg ttctggcacc cacacctttt ccgcctgcgc caaagagtag
     5881 ccacggcgtc atcaggcgtg ttcctttaac gatgccgcca gagtatggaa ggcgctgact
     5941 tgttcgctca ggagctgacg atggtcatcc ctgccgagga agattttcag catggcgctg
     6001 ccttctttgt tgaaaaagag aatggacgcg gtgtccatgc ccataaattt acgttcgatt
     6061 aaggcaatat gcgtgcagtt ttctgctttg atatgcccgg acatgccgtg ctttccgcgt
     6121 aggttgaaat agccgtggcg atggaaaccc gaaggcagtt cgccgctaaa ttcgaggatt
     6181 acatcggcag tatgtactaa cgtggtgacg ttgccccatt cgcagacggt atcccagaca
     6241 gtatcgaatt tatcgcctgg cactacggta gaggagggta gatttctcac tacctccagc
     6301 agtgtggtgt tgtattgttc ggctaccacc tccagcgtac cgtccggctc cgttttcaaa
     6361 aattcttgta acgagacatg gctcataaat cactcctgtt atggtttttc acgcataatc
     6421 gctggcgcat taagtacctg aattagttca ttaagagact gcaaaatatt gcttgcccag
     6481 aaacgacctt cattagtcag ccgcaggcaa gttgaagcat cgcgacttaa gcctttttgg
     6541 tgccattgag ccagtaacgg cgcgagtttt tcggcatgtg gcgtaagttc atccagcggt
     6601 acgcgcgctg tttcaacacc tgactgcaag gtatgacgcc attgcgcatt acgttcggcg
     6661 ttacgcatga tcaacatcag cggttttttg cctgccgcga cggattcatg ccaggtctga
     6721 agattgcgtt cgttcatcca ggagtaacca ttaatcgaac cgccggctcc ggaaccaaag
     6781 gccagacaat cggcaccttg tttaatcagc aggttataga gattgcgttc gcgtgtggta
     6841 cggccccagt ggctgttact gatgcagcgc caaccggcat cgtccataaa atcacatcct
     6901 tgcagataaa gatcgcgacg ttctgccggt gagggtacgg tagtacgccc attttccacg
     6961 gccttgccca gcggtgtatt ggacaggaca ttgagcgcat agagatcgac gccgtcgaga
     7021 ccgatatcgc gggcaatagc cagatcttct ccccaggttt gcgcatcctg acccggcagg
     7081 ccaaacagca ggtcacagac caccgcagca cggtcacgtt tgaccaggct ttccataaac
     7141 gcaatagcgg ttgggccatc cgaggtgcgg gccattttct tgcggatttt gctgttaaac
     7201 gactggatac caattgagaa gcggtttgct cctgcatcaa ggcaagcatc gattcgctca
     7261 gcgtcaaaat tcaatacccg tccttcgata gtgatttcac aatcaggggc cagtggcagc
     7321 ttttctcgta atgtggtgat gattctggcc aaatcgtgtg ccgaaagtgc ggaaggcgta
     7381 ccgccaccga aatagaccgc gtggatgggg gcagactgat gcaatacgct gtccgcttcc
     7441 atttctattt cacgaatcag ggcgtcggta taatgagcac acgcatcttc attaaaacgg
     7501 ttctgataaa aaccacaaaa cgtgcagtgt gtcgcacaaa aaggaatgtg taggtaaacc
     7561 agtcgcttac gaggtgatgc cgtttgattg atcatctctt gccaggtctg tgccagttgc
     7621 tctttggcaa ccggaatggc tccacgaaac ggcatcatgg cgcgcctgtc tttaaatggc
     7681 tgatcaccgt ctaaagcaaa atgtggcgtg agatccaggg tgttatttgc gttcattcgt
     7741 ctgatttaaa tccatttatt gcggccaaag ctgctggtgt aaagactcaa cgacatcagc
     7801 aatacgcgga cccattccca gaatcaggtt ttgatcaacg gtgataatgc gctggttttt
     7861 ccatgcggca gtgtgggtaa ttccagcaat agaacgtagc cgattaatat cgccatcgac
     7921 catttgtgag gttacgacaa tcacttctgg attagccgca atcagtgatt ctgcactgta
     7981 gcttttgtac tgctggtggg tagcgacgtt ttctgctccg gcaagcgaaa gaatggcgtc
     8041 cgcgacgctg cctttaccgg caacttgtgg cgcacttccg cctgcggaga gaataaacat
     8101 ggctttaacc ggggcttttt tggtcgccac gttttgctgt actcgctcca ggcgttggct
     8161 gatttgtgtc accagcgcct cgccttgttc aggaacctgt agcgtttttg ccagttggcg
     8221 aatgttggcg tacatctgct gaagggtggc aggcacacgc ggcagggtga cgacgttgac
     8281 tttctgcgct cgtagttggt cgagcacaat ttgtggcccg gcatcctgcc aggtaattac
     8341 gctatccggg cgaagtgaca aaatgccttc gctgctgagt tgtttccagt agccaatatg
     8401 cggcagtttg gcggtttctg gtggataaga tgtcgtttca tcgacaccaa ccacgcgttc
     8461 gccagcgccc atcgcgtaga ttagctccgt cagcgatcct cctgcgacca cgatacgttc
     8521 ggcagccgtt acgctaaaag cccagctaag agccagtact gccgtaaaca attttttacg
     8581 ttttactgcg gtcttcgcgg cagaggccac ggctgaaata cataacgtca gtggggaaaa
     8641 aagaaacggc ctggtgatga tccttggcat aatatgagaa ctcttatcaa attgagaat
//
LOCUS       DAZGXS010000163         8696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGXS010000163 DAZGXS010000000
VERSION     DAZGXS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(342..605)
                     /gene="rpsT"
                     /locus_tag="ACHKG5_004171"
     CDS             complement(342..605)
                     /gene="rpsT"
                     /locus_tag="ACHKG5_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0870163.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            708..932
                     /locus_tag="ACHKG5_004172"
     CDS             708..932
                     /locus_tag="ACHKG5_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP0870164.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            940..1881
                     /gene="ribF"
                     /locus_tag="ACHKG5_004173"
     CDS             940..1881
                     /gene="ribF"
                     /locus_tag="ACHKG5_004173"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0870165.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1924..4740
                     /gene="ileS"
                     /locus_tag="ACHKG5_004174"
     CDS             1924..4740
                     /gene="ileS"
                     /locus_tag="ACHKG5_004174"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0870166.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4740..5234
                     /gene="lspA"
                     /locus_tag="ACHKG5_004175"
     CDS             4740..5234
                     /gene="lspA"
                     /locus_tag="ACHKG5_004175"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0870167.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5322..5771
                     /gene="fkpB"
                     /locus_tag="ACHKG5_004176"
     CDS             5322..5771
                     /gene="fkpB"
                     /locus_tag="ACHKG5_004176"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0870168.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5773..6723
                     /gene="ispH"
                     /locus_tag="ACHKG5_004177"
                     /gene_synonym="lytB"
     CDS             5773..6723
                     /gene="ispH"
                     /locus_tag="ACHKG5_004177"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0870169.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6789..7703
                     /gene="rihC"
                     /locus_tag="ACHKG5_004178"
     CDS             6789..7703
                     /gene="rihC"
                     /locus_tag="ACHKG5_004178"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0870170.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7870..8691
                     /gene="dapB"
                     /locus_tag="ACHKG5_004179"
     CDS             7870..8691
                     /gene="dapB"
                     /locus_tag="ACHKG5_004179"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0870171.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1994 a         2170 c         2459 g         2073 t
ORIGIN      
        1 taataattta caaggtctaa tgaggagata tttatgaatg gttaaagtat ataggaattt
       61 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
      121 ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      181 ttctacgatt cttaagccac gaagagttca gatagtgcaa cggcatgtct cttttgacta
      241 tctggcaacc ggcagtgtgt tctctcacgc atcacaaaag cagcaggcat aaaaaaaccc
      301 gcttgcgcgg gctttttcac aaagcttcag caaattggca attaagccag tttgttgatc
      361 tgtgcagtca ggttagcctt atgacgtgca gctttgtttt tgtggatcag acctttagca
      421 gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt tctgtgcagc agctttgtcg
      481 ccagcttcga tagctgcgta tactttcttg atgaaagtac gcatcataga gcgacggctt
      541 gcgttgtgct tacgagcctt ttcagactga atggcgcgct tcttagctga tttgatatta
      601 gccaaggtcc aactcccaaa tgtgttctat atggacaatt caaaggccga ggaatatgcc
      661 cttttagcct tcttttgtca atggatttgt gcaaataagc gccgttaatg tgccggcact
      721 cgttacgtag tgatggcgca ggattctacc agcttgcggg gtgtgaatac agcttttccg
      781 cgataaaaat tgcagcaggc agtcagtttc ttcccgtgat ttgcgccatg accatgacaa
      841 tgaaaagcca cttctttctg atttcggtac tcaatcgccg gttaaccttg accgctgtac
      901 aaggtatact cggacgattt tcactgtttt gagccagaca tgaagctgat acgcggcata
      961 cataatctca gccaggcccc gcaagaaggg tgtgtgctga ctattggtaa tttcgacggc
     1021 gtgcatcgcg gtcatcgcgc gctgttacag ggcttgcagg aagaagggcg caagcgcaac
     1081 ttaccggtga tggtgatgct ttttgaacct caaccactgg aactgtttgc taccgataaa
     1141 gccccggcac ggctgacccg actgcgggaa aaactacgtt accttgcaga gtgtggcgtt
     1201 gattacgtgc tgtgcgtacg tttcgacagg cgtttcgcgg cgttaaccgc gcaaaatttc
     1261 atcagcgatc ttctggtgaa gcatttgcgg gtaaaattcc tcgccgtggg ggatgatttc
     1321 cgctttggcg ctggtcggga aggcgatttc ttgttattac agaaagctgg catggaatac
     1381 ggcttcgata tcaccagtac gcaaactttt tgcgaaggtg gcgtgcgtat cagcagcacc
     1441 gccgtgcgtc aggcccttgc ggatgacaat ttggctctgg cagaaagttt actggggcac
     1501 ccgtttgcca tctccgggcg tgtagtccac ggtgatgaat tagggcgcac tataggtttc
     1561 ccgacggcga atgtaccgct acgccgtcag gtttccccgg tgaaaggggt ttatgcggta
     1621 gaagtgctgg gcctcggtga aaagccgtta cccggcgtgg caaacatcgg aacacgccca
     1681 acggttgccg gtattcgcca gcagctggaa gtgcatttgt tagatgttgc aatggacctt
     1741 tacggtcgcc atatacaagt agtgctgcgt aaaaaaatac gcaatgagca gcgatttgct
     1801 tcgctggacg aactgaaagc gcagattgcg cgtgatgaat taaccgcccg cgaatttttt
     1861 gggctaacaa aaccggctta agcctgttat gtaatcaaac cgaaacacgg aaccgagaat
     1921 ctgatgagtg actataaatc aaccctgaat ttgccggaaa cagggttccc gatgcgtggc
     1981 gatctcgcca agcgcgaacc gggaatgctg gcgcgttgga ctgatgatga tctgtacggc
     2041 atcattcgtg cggctaaaaa aggcaaaaaa accttcattc tgcatgatgg ccctccttat
     2101 gcgaatggca gcattcatat tggtcactcg gttaacaaga ttctgaaaga cattatcgtg
     2161 aagtccaaag ggctttccgg ttatgactcg ccgtatgtgc ctggctggga ctgccacggt
     2221 ctgccgatcg agctgaaagt agagcaagaa tacggtaagc cggatgagaa attcaccgcc
     2281 gccgagttcc gcgccaagtg ccgcgaatac gcggcgaccc aggttgacgg tcaacgcaaa
     2341 gactttatcc gtctgggcgt gctgggcgac tggtcgcacc cgtacctgac catggacttc
     2401 aaaactgaag ccaatatcat ccgcgcgctg ggcaaaatca tcggcaatgg tcacctgcac
     2461 aaaggcgcga agccggtgca ctggtgcgta gactgccgtt ctgcactggc agaagcggaa
     2521 gttgagtatt acgacaaaac ttctccgtcc attgacgtcg ctttccaggc ggtcgatcag
     2581 gatgcgctga aagcgaaatt tggcgtaagc aacgttaacg gcccaatctc gctggtgatc
     2641 tggaccacta cgccgtggac tctgcctgcg aaccgcgcaa tctctattgc acctgatttc
     2701 gactatgcgc tggtgcagat cgacggtcag gccgtgattc tggcgaaaga tctggttgaa
     2761 agcgtaatgc agcgtatcgg cgtgaccgat tacaccattc tcggcacggt aaaaggtgcg
     2821 gagcttgagc tgctgcgctt tgcccatccg tttatgggct tcgacgtccc ggcaatcctc
     2881 ggcgatcacg ttaccctgga tgccggtacc ggtgccgttc acaccgcgcc tggccacggc
     2941 ccggacgact atgtgatcgg tcagaaatac ggcctggaaa ccgctaaccc ggttggcccg
     3001 gacggcactt atctgccggg cacttatccg acgctggatg gcgtgaacgt cttcaaagcg
     3061 aacgacatcg tcgttgcgct gctgcaggaa aaaggcgcgc tgctgcacgt tgagaaaatg
     3121 cagcacagct atccgtgctg ctggcgtcac aaaacgccga tcatcttccg cgcaacgccg
     3181 cagtggttcg tcagtatgga tcagaaaggt ctgcgcgcgc agtctctgaa agagatcaaa
     3241 ggtgtgcagt ggatcccgga ctggggccag gcgcgtatcg agtcgatggt cgctaaccgt
     3301 cctgactggt gtatctcccg tcagcgtacc tggggcgtac cgatgtctct gttcgtgcac
     3361 aaagacacgg aagagctgca tccgcgtacc ctcgaactga tggaagaagt ggctaaacgc
     3421 gttgaagttg atggcatcca ggcgtggtgg gatcttgatg cgaaagagat cctcggcgat
     3481 gaagctgatc agtatgtgaa agtgccggat acgctggatg tatggtttga ctccggctct
     3541 actcactctt ctgttgttga cgtgcgcccg gaatttgccg gtcacgctgc ggacatgtat
     3601 ctggaaggtt ctgaccaaca ccgtggctgg ttcatgtctt ctctgatgat ctccaccgcg
     3661 atgaagggca aagcgccgta tcgtcaggtg ctgacccacg gctttaccgt agatggtcag
     3721 ggccgcaaga tgtctaaatc catcggcaac accgtgtcgc cgcaggatgt gatgaacaaa
     3781 ctgggcgcgg atattctgcg tctgtgggtg gcatcaaccg actacaccgg tgaaatggcc
     3841 gtttctgacg agatcctgaa acgtgctgcc gacagctatc gtcgtatccg taacaccgcg
     3901 cgcttcctgc tggcaaacct gaacggtttt gatccggcaa aagatatggt gaaaccggaa
     3961 gagatggtag tactggatcg ctgggccgta ggttgtgcga aagcggcaca ggaagacatc
     4021 ctcaaggcat acgaagcata cgatttccac gaagtggtac agcgtctgat gcgcttctgc
     4081 tccgttgaga tgggttcctt ctacctcgac atcatcaaag accgtcagta caccgccaaa
     4141 gcggacagcg tggcgcgtcg tagctgccag actgcgctgt atcacatcgc agaagcgctg
     4201 gttcgctgga tggcaccaat cctctccttc accgctgatg aagtgtgggg ctacctgccg
     4261 ggcgaacgtg aaaaatacgt cttcaccggc gagtggtacg aaggtctgtt tggtctggca
     4321 gacagtgaag caatgaacga tgcgttctgg gacgagctgt tgaaagtgcg tggcgaagtg
     4381 aacaaagtca ttgagcaggc gcgtgccgac aagaaagtgg gtggctcgct ggaagcggca
     4441 gtaaccttgt atacagaacc ggaactggcg gcgaaactga ccgcattggg cgatgaatta
     4501 cgatttgtcc tgttgacctc cggcgctacc gttgcagact ataacgacgc acctgctgat
     4561 gctcagcaga gcgaagtgct caaagggctg aaagtcgcgt tgagtaaagc cgaaggtgag
     4621 aagtgcccac gctgctggca ctacacccag gatgtcggca aggtggcgga acacgcagaa
     4681 atctgcggcc gctgtgtcag caacgtcgcc ggtgacggtg aaaaacgtaa gtttgcctga
     4741 tgagtcaatc gatctgttca acagggctac gctggctgtg gctggtggta gtcgtgctga
     4801 ttatcgatct gggcagcaaa tacctgatcc tccagaactt tgctctgggg gatacggtcc
     4861 cgctgttccc gtcgcttaat ctgcattatg cgcgtaacta tggcgcggcg tttagtttcc
     4921 ttgccgatag cggcggctgg cagcgttggt tctttgccgg tattgcgatt ggtattagcg
     4981 ttaccctggt tgtgatgatg tatcgctcga aggccacgca gaagctaaac aatatcgcct
     5041 acgcgctgat tattggcggc gcgctgggca acctgttcga ccgcctgtgg cacggcttcg
     5101 ttgtcgatat gatcgacttc tacgtcggcg actggcactt cgccaccttc aacctcgccg
     5161 atactgccat ctgtgttggt gcggcactga ttgtgctgga aggttttttg ccttctaaag
     5221 cgaaaaaaca ataataaacc ctgccggata aggcctacag attgctgcga aatcgtaggc
     5281 cgcatccggc aaaaatcctc aaaatataag agcaaacctg catgtctgaa tctgtacaga
     5341 gcaatagcgc cgtcctggtg cacttcacgc taaaactcga cgatggcacc accgccgagt
     5401 ctacccgcaa caacggtaaa ccggcgctgt tccgcctggg cgatgcttct ctttccgaag
     5461 ggctggagca acacctgctg gggctgaaag tgggcgataa aaccactttc tcgctggagc
     5521 cagatgcggc gtttggcgtg ccgtcaccgg acctgattca gtacttctcc cgccgtgagt
     5581 ttatggatgc aggcgagcca gaaattggcg caataatgct ttttaccgca atggatggca
     5641 gtgagatgcc tggcgtgatc cgcgaaatta acggcgactc cattaccgtc gatttcaacc
     5701 atccgctggc cgggcagacc gttcattttg atattgaagt gctggaaatc gatccggcac
     5761 tggaggcgta acatgcagat cctgttggcc aacccacgtg gtttttgtgc cggggtagac
     5821 cgcgctatca gcattgttga aaacgcgctt gccatttacg gcgcaccgat atatgtccgt
     5881 cacgaagtgg tgcataaccg ctacgtggtc gatagcctgc gcgagcgtgg agctatcttt
     5941 attgagcaga tcagcgaagt gccggacggc gcgatcctga tcttctccgc acatggtgtt
     6001 tctcaggcgg tacgtaacga agcgaaaagc cgtgatttga cggtattcga cgccacctgt
     6061 ccgctggtga ccaaagtgca tatggaagtc gcccgcgcca gccgtcgtgg cgaagagtct
     6121 attctcatcg gtcacgccgg gcacccggaa gtggaaggga cgatggggca gtacagcaac
     6181 cctgaagggg gaatgtatct ggtcgaatcg cctgacgatg tgtggaaact gacggtcaaa
     6241 aacgaagaga agctctcctt tatgacccaa accacgctgt cggtagatga cacgtctgat
     6301 gtgatcgacg cgctgcgtaa acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc
     6361 tgctacgcca cgactaaccg tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt
     6421 gtgttggtgg tcggttcgaa aaactcctcc aactccaacc gtctggcgga gctggcccag
     6481 cgtatgggca aacgcgcgtt tttgattgac gatgcgaaag atatccagga agagtgggtg
     6541 aaagaggtta aatgcgtcgg cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag
     6601 aatgtggtgg cacgtttgca gcagctgggt ggtggtgaag ccattccgct ggaaggccgt
     6661 gaagaaaata ttgttttcga agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat
     6721 taagtcatta gcagcctaag ttatgcgaaa atgccggtct tgttatcggc attttttatg
     6781 gagaaaacat gcgcttacct atcttcctcg atactgaccc cggcattgac gatgccgtcg
     6841 ccattgccgc cgcgattttt gcccccgaac tcgacctgca actgatgacc accgtcgcgg
     6901 gtaatgtctc ggttgagaaa actacccgta acgccttgca actgctgcat ttctggaatg
     6961 cggagattcc gctcgcccag ggagccgctg tgccgctggt acgcgcaccg cgtgatgcag
     7021 catctgtgca cggcgaatcg ggaatggctg gctacgactt tgttgagcac aaccgaaagc
     7081 cactcgggat accggcgttt ctggcgattc gggatgccct gatgcgtgca ccagagcccg
     7141 ttaccctggt ggccatcggc ccgttaacca atattgcgct gttactttca caatgcccgg
     7201 aatgtaaacc gtatattcgc cgtctggtga tcatgggtgg ttctgccgga cgcggcaact
     7261 gtacgccaaa cgccgagttt aatattgctg ccgatccaga agctgctgcc tgtgtcttcc
     7321 gcagtggtat tgaaatcgtc atgtgcggtt tggatgtcac caatcaggca atattaactc
     7381 ctgactatct ctctacactg ccgcagttaa accgtaccgg gaaaatgctt cacgccctgt
     7441 ttagccacta ccgtagcggc agtatgcaaa gcggcttgcg aatgcacgat ctctgcgcca
     7501 tcgcctggct ggtgcgcccg gacctgttca ctctcaaacc ctgttttgtg gcagtggaaa
     7561 ctcaaggcga atttacctca ggcacgacgg tggttgatat cgacggttgc ctgggcaaac
     7621 cagccaatgt acaggtggca ttggatctga atgtaaaagg cttccaacag tgggtggctg
     7681 aggtgctggc tctggtgccg taacctgtca catgttatcg gcatgcagtc attcatcgac
     7741 tcatgccttt cactgatatc cctccctgtt tatcattaat ttctaattat cagcgttttt
     7801 ggctggcggc gtagcgatgc gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag
     7861 agaatagcta tgcatgatgc aaacatccgc gttgccatcg cgggagccgg ggggcgtatg
     7921 ggccgccagt tgattcaggc ggcactggca ttagaaggcg tgcagctggg cgcggcgctg
     7981 gagcgcgaag gatcttcttt actgggcagc gacgccggtg agctggctgg tgcagggaaa
     8041 acaggcgtta ccgtgcaaag cagtcttgat gcgataaaag atgattttga tgtgtttatc
     8101 gattttaccc gtccggaagg tacgctgaac catctcgctt tttgtcgcca gcatggcaaa
     8161 gggatggtga tcggcactac ggggtttgac gaagccggaa aacaggcaat tcgtgacgcc
     8221 gccgccgata ttgcgattgt ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt
     8281 aagctgctgg agaaagcagc caaagtgatg ggtgactaca ccgatattga aattattgaa
     8341 gcacatcata gacataaagt tgatgcgccg tcaggcaccg cactggcaat gggagaggcg
     8401 atcgcccacg cccttgataa agatctgaaa gattgcgcgg tctacagtcg tgaaggccat
     8461 accggtgaac gtgtgcctgg taccattggt tttgccaccg tgcgtgcagg tgacatcgtt
     8521 ggcgaacata ccgcgatgtt tgccgatatt ggcgagcgtc tggagatcac ccataaggcg
     8581 tccagccgca tgacatttgc taacggtgcg gtaagatcgg ctttgtggtt gagtggtaag
     8641 gaaaatggtc tttttgatat gcgagatgtg cttgatctca ataatttgta accaca
//
LOCUS       DAZGXS010000164         8671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGXS010000164 DAZGXS010000000
VERSION     DAZGXS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..359
                     /locus_tag="ACHKG5_004180"
     CDS             <1..359
                     /locus_tag="ACHKG5_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870172.1"
                     /translation="KQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIINTDLV
                     IGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIK
                     DNHNTPWLLNNENVQE"
     gene            complement(1230..1370)
                     /locus_tag="ACHKG5_004181"
     CDS             complement(1230..1370)
                     /locus_tag="ACHKG5_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0870173.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1672..1935)
                     /locus_tag="ACHKG5_004182"
     CDS             complement(1672..1935)
                     /locus_tag="ACHKG5_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0870174.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2360..2485
                     /locus_tag="ACHKG5_004183"
                     /pseudo
     CDS             2360..2485
                     /locus_tag="ACHKG5_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            2550..2810
                     /locus_tag="ACHKG5_004184"
                     /pseudo
     CDS             2550..2810
                     /locus_tag="ACHKG5_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2860..3099
                     /locus_tag="ACHKG5_004185"
                     /pseudo
     CDS             2860..3099
                     /locus_tag="ACHKG5_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3147..3764)
                     /gene="ureG"
                     /locus_tag="ACHKG5_004186"
     CDS             complement(3147..3764)
                     /gene="ureG"
                     /locus_tag="ACHKG5_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP0870175.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3776..4450)
                     /locus_tag="ACHKG5_004187"
     CDS             complement(3776..4450)
                     /locus_tag="ACHKG5_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP0870176.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(4451..4915)
                     /gene="ureE"
                     /locus_tag="ACHKG5_004188"
     CDS             complement(4451..4915)
                     /gene="ureE"
                     /locus_tag="ACHKG5_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP0870177.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4925..6628)
                     /gene="ureC"
                     /locus_tag="ACHKG5_004189"
     CDS             complement(4925..6628)
                     /gene="ureC"
                     /locus_tag="ACHKG5_004189"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP0870178.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(6621..6941)
                     /locus_tag="ACHKG5_004190"
     CDS             complement(6621..6941)
                     /locus_tag="ACHKG5_004190"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP0870179.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6950..7252)
                     /locus_tag="ACHKG5_004191"
     CDS             complement(6950..7252)
                     /locus_tag="ACHKG5_004191"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP0870180.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(7343..8041)
                     /locus_tag="ACHKG5_004192"
     CDS             complement(7343..8041)
                     /locus_tag="ACHKG5_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP0870181.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            8422..>8671
                     /locus_tag="ACHKG5_004193"
     CDS             8422..>8671
                     /locus_tag="ACHKG5_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870182.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGII"
BASE COUNT         2193 a         2179 c         2109 g         2190 t
ORIGIN      
        1 taaaacagac gtctgtgcct gattttattt attattcaat agaaaaacac aacaaatatc
       61 ccatatatgt attacacatg ccatcaaaca atataattga aataggctac aatataataa
      121 acaccgatct ggtcattggc gaaaaaattc atttcaggac actaagcaat cgttctttat
      181 attttaacct cacccaaccc ccattaatcg ccacaataaa agatgatgtg ttttgcactc
      241 ttcatgatta ctataaccac aataatgaaa cgaaatccac tattgacaat tacatatcaa
      301 aaataaagga taatcataat accccatggt tattaaacaa cgaaaatgtt caggaataaa
      361 aaaaagagac aattcccgag ataaaacaaa agagcataaa catatgaacc ggaaattcta
      421 tttcaaaaat atcctgtggg gaatgtgccc cattataata tccatattgc atctgatata
      481 tgacacacca acagaagcat cgataaaagt tgacttattt ctgatactca gtggcctggc
      541 ccttccttgc tcaatatacc tgacaaaaac aatcacacta aaatacacat ccatatcatt
      601 ctggaaaaaa cctcttttct ggtcttatgg tatatatcat tttcatcttg tttatataat
      661 gatatgtata gttttagcaa taccttctgc aattatcgtt ttattctttc acttttatca
      721 aaacatcacc agaaactgga agtgacaacc gcaataatga acataaaaag gcaaatcaga
      781 atggcagtaa atcaataaag taccatttca tatgaatcat cagaaaaacg gtggacgcga
      841 catccagcac gaggcaaaac acagcaacaa aataagccgg atatcgcgcc ctgtactttt
      901 ctaactcaac tcccggcatt gtgcaagttt taatcgcagg tggattcaga gccacgcccc
      961 cattgattca gcctgaagac acgtatatcc ttggagaaag caggaatctc attgctgtga
     1021 aattccttac agaatcttca gccccatttt atggggcttt ttttggtgcc cccacgctgt
     1081 taccacactt tcgggatgcg aataaaaaag cccacagttt ttacactgcg gacttttgtt
     1141 ttttctttgt gatttgtgtg caagggtgcg actttcgacc gaacctttgg caaccggata
     1201 gcggggactg ttgtccccgc tgcggtttct tacggtttac actgcaagaa cgccgcaatt
     1261 tccgaccaca tgaagcttac cctgaattca cacggtgatg cgtgtaacac ccagacggtc
     1321 agcaacatgg tcaggcataa catgcccaca atgatcgttt tttgcggcat agcacttgct
     1381 ccttttacag agaggcgcta accttccact tgctgaaggt agatggtcag ggcctcgggt
     1441 taacgtttaa gttgactcgg ggcctttcca catccggcct tcaggtaatc cttccagccg
     1501 tcagccgaaa ggcacccgcg cgtaatgtac tggttttcag agaggtccgc aatcttccgg
     1561 tgacgagatc atctgcacac gccccggtgg gacgggctaa cggtatattc aggtgcagga
     1621 tctctctgtc tggtgcgttt ttaaattgtc aaacgcaccc tggcggcaaa atcagccacc
     1681 ttttttcgca tcaacaaaac gcccaaaacg ctgggtaaag cgtgcaacat taccttctga
     1741 agcgacagtt ctcagtttac ctgtataaaa cggatgcgac ttagaagaaa cgtcaattgt
     1801 aacatatggg taagttacac catccagttc aattacccgg tcagttttga tcgtcgaacc
     1861 gatcctgaag tactcatcaa tactggtatc gtgaaatact actgtacggt attccggatg
     1921 gatattcggt ttcacaacag ttttccccct ttaagtgaaa attccgcata aaggtacata
     1981 ttcagacggt cagattcaac tgtctgttct tataacctca ctctctttca gtattacaat
     2041 atgttatata attataaact ggacttcccg aaaaaatcca ttaaataaga agcgagccgt
     2101 tgcggaggct tgctctctcc tttaacctca tgtgcaatat ccgtaacgcg ctcacataaa
     2161 tcatcatctg ataatataac aacacccatt attttattct gacctaaaaa cttcttatga
     2221 aataaacgtt tacccgacgt atctgatccc gctgttttta cctgtatcct ttaacatact
     2281 tgagaagccc gctgaaaaaa tataccgtaa aggcggtttc tctgtcttcc ataaacctgt
     2341 cggataagaa gggcattaaa tgaaaaccaa aaaagttatg atgattgcac tggtcagctc
     2401 tgcgctggct ctttcaggat gtggagccat gggcacagcg atcaaaaaac gaaatctgga
     2461 agtaaaaacc cagatgagtg aaacctgagg tagcctgagt ttaacggaca ctccttcctg
     2521 aaatagaatg gcatcagaag gagctaataa tgagcagaaa aacccaacgt tactctaaag
     2581 agttcaaagc cgaagctgtc agaacggttc ttgaaaatca actttcgatc agtgaaggcg
     2641 cttcccgatt atctcttcct gaaggcactt taggacaatg ggttaccgcc gccagaaaag
     2701 ggctcggtac tcctggttcc cgcacggtgg ctgaactgga atctgaaatt ctgcaactgc
     2761 gtaaggcgtt aaatgaagct cgccttgagc gagatatatt aaaaaaagca gctgtagatt
     2821 caatctgtca atgcaacacc cctttcaatt atctctttca ctgcccgcga cggtatcggt
     2881 tatcagtttg acctgctggc taacggtgaa accgtactgc gtgatgcatc cggcgaaaaa
     2941 cgcatcaagg gtgaaaaaga cggtcgtatg ctcatgaatg ttggcctgaa tgcggaaatt
     3001 cgcgacaaca tccgcttcgg tctggagttt gaaaaatcgg catctggtaa gtacaacgtg
     3061 gataacgcgg tgaacgccaa cttccgttat tccttctgat aaaaacaagg ggccgcctgc
     3121 cggccctgat acagaggatg ttgtgattac ttacccagca tgccctgctc ttcgataaag
     3181 gcgatgatgt tttgcagacc ttcaccactt ttcaggttag tgaaccccca tgggcgttct
     3241 ccgcgcatac gtaaagtatc acgctccatt acctcgaggg aggcgcccac gtatggtgca
     3301 aggtcagttt tattgatcac gaggaagtcg gatttggtga tccccggacc gcctttacgc
     3361 gggatctttt ctccttctgc cacatctatg acatatatgg taaggtccgc cagttcgggg
     3421 ctgaaggtcg cactcaggtt gtcgccgccg ctttccacaa agatcagctc gagattacca
     3481 aatttctcac tcagtgcttc cacggctgcc agattcattg atgcgtcttc acgaatggct
     3541 gtatgagggc agccccccgt ttcgacaccg acaatgcgtt ccgcttccag tgctccggcc
     3601 tcagtgagaa tgcgttgatc ttctctggtg tagatgtcgt tggtgactac ggctaattgc
     3661 caggtgtcac gcatggattt gcagagggct tccagaagcg cggttttacc tgagccgacc
     3721 ggaccgccga cgccgacacg cagcggttgc ttgattatgt tcattttatt ttctcctaag
     3781 aacggaataa tcgggaatac tgggtttcat gtcgcgcgga agcgatagat gcaagtggcg
     3841 ttgctgaacc aattgcgtga tccggggttg caagggcact gtccatgttc gccgcatagc
     3901 gggcacacag gcgcataatt aactgctggg cagccagctg gccgtagggt accagcctga
     3961 cgcccgccat cactgcgctt tcaatccagc tgtagccaag gctcagggcc aggtctgaaa
     4021 cggatatttg ccagcgtacg ccgagccatg ccataccggt taactggctt tgctggcata
     4081 gtttgcgcca ttcggccggg cagtcgggct gccagtcgac cagaagtctg gtgaaggctg
     4141 caccgcggtt acgttcttca tcgcgtagct cacgggtttc cctgcaggcc agcagataag
     4201 cggtccagcg cccggcactg tcaggatcgc ccgcttcaca ggcccggtat agccgggcaa
     4261 gcagtggcag gtcgacggca aaaaaactct gttccatctg caactgctgc cagtgttcaa
     4321 aggcggcgct atcttctacc cagcctgctt ccactgccca ctccagtccc tgggaccagc
     4381 tgtagcctcc aacagggagg ctgttactgg caagctgcat cagccgcaga cgtttttccg
     4441 gagtcggcat tcagtggctg tgctcctgat cgtgattatg ggcgtgcgac ttgctggcgt
     4501 aagcgccggc ttccggctca aacggaagat gtgcaaaatc aacatccagc ccaaactggc
     4561 gcagcatatc atcgaggacg tgatcgtgat ggtagcgcag ctctccgggc attatttgca
     4621 gcggaacgtg gcgattgccg agatgccagc aggctttcgc gagcataaaa ggatctgcgc
     4681 agcgcactac agaaacggct tcatcagcgg cgatcacctt aatgaattca tcaccgtttt
     4741 cgttactgag aatatcacca tcgcgcagca gcagaccgcg tggcagcaac agtccagcct
     4801 gacgtccatc gttaagggtg actttaatac ggctttttac ccgcatgtca acagggaggg
     4861 taacgctggc agttgtctga gccggggtct ccacccgccg ggtcaaataa agcatttgtg
     4921 ctccttaaaa caggaaataa cgttgcgcca ttggcagaat gtccgccggt tcactggtta
     4981 tcagttcgcc gttgatacgc acttcgtagg tttgtgaatc cacggttata tcaggcaaca
     5041 ggctgttgtg gcgcatatcg gctttttgta ctgtgcggca gccttttacc acagcagttg
     5101 tgctgtgcag gttaagctgt tcagcgacgc catttgctgc tgctgcctgc gacaggaaag
     5161 tcacacgaca gcggtgacgg gcactgccca atgcagcgaa cattgggcga tagtgcaccg
     5221 gctgcggtgt ggggatagag ccgttgatat cacccatcgg cgccatggct atcattccgc
     5281 ctttgacgat agtcgccggt tttacgccaa agaacgccgg ggaccagagc accagatccg
     5341 ccagttttcc cacttcaatc gagccgactt catgagcaat accgtgggtt aatgccggat
     5401 taatggtgta tttagcgata tagcgcttca cgcggacgtt gtcgttatca ccactttctt
     5461 ccggtaacgg gccgcgctga actttcatcc ggtgtgccac ctgccaggtt cgtaacacta
     5521 cttctccgac gcgtcccatg gcctgcgaat cggacgaggt gagggagaac gcgccaaggt
     5581 cgtgcaggac gtcttccgcg gcaatggttt cctggcgaat gcgcgattcg gcaaaggcta
     5641 cgtcctcggc gatatccggg tcaagatgat ggcaaaccat cagcatgtcc agatgctcat
     5701 caatagtgtt gacggtatag ggtagcgtcg gattggttga ggaaggcaga atattggggt
     5761 gcgcgcaggc ggtgataata tccggagcat gaccaccacc tgcaccttct gtatggaagg
     5821 tatggatagt gcgcccgccg atggcagtca gagtatcctc aacgaatcct gattcgttaa
     5881 gcgtatcact gtgtagcgca acctgaacgt ccatttcgtc ggctacagtc agtgcgcagt
     5941 tgattaccgc aggtgtagct ccccagtctt cgtgaatttt gaggccgata accccggccg
     6001 cgacctgctc acgaagcgca tccggattgg agcaattgcc tttacccagc aacccgatat
     6061 tgaccggcag gctgtctgca gcctgcagca tttgataaat gtaccatggt cctggggtac
     6121 aggttgtggc gttagaaccc gctgtcgggc cagtaccgcc accgatcatg gtggtaatgc
     6181 cggatgtcag cgcttcttca gcctgctgtg ggcagatcca gtgaatatgc gtatcgacgc
     6241 cacctgcggt aacgatcctg ccttctgcgg caataatttc cgtggatacg ccgattggga
     6301 tagtgacgtt gggttgtata tcaggattac cggctttgcc gatagcaaat atccttccat
     6361 ctttgacgcc gatatcggct ttaacgatcc cccagtaatc gatgatcagg gcattggtca
     6421 gcaccagatc agcgcagccg gcggagagca tttgcccctg tcccataccg tcgcggatta
     6481 ctttaccgcc gccgaatttg acctcttcgc cgtaggtagt taaatcatct tcgacctcga
     6541 tccacagctc agtgtctgcc agacgaattt tatcaccggt ggtagggccg aacatgtcag
     6601 cataggcctg gcgtgaaata ttactcatca tttgcctcca gctcacccat tatatctccg
     6661 cggaaaccgt gcacgatacg ggcaccgctc agcgccacca gttcaacctc acgcttttgg
     6721 cctggttcga agcgcactgc agttccggcg gcgatattga gacgatagcc tcttgccttc
     6781 tggcgatcga acttcagtgc cgggttaacc tccgcgaagt gataatggga accgacctga
     6841 attggtcggt ctccgtgatt ttcaacgata atactgcagg tagcccggcc gatgttaagc
     6901 tctatatagc caggtttaac cttatattct ccagggatca tctgttacct cagattatcg
     6961 gattatggac ggtaaccagc ttggagccgt ccggaaaggt ggcttcgacc tggatatcgg
     7021 ggatcatttc tggtatgcct tccatgacct gctcgcgact caggacatgc cgtccttctt
     7081 ccatcagcgc agcgacgctt ttgccgtcgc gagcgccctc cattataaaa gcgctaatca
     7141 gggccacgga ttcgggatag ttaagtttca ggccgcgggc cagacgacgc tctgccagca
     7201 gtgcagcggt aaacagtaat agtttgtctt tttctcttgg agtcagttcc atagcgttct
     7261 cttaagtcag ccagatccgg ggaagtaccg gtgatttgtc agtcagttgc ggccgcataa
     7321 attgccagat atcacgcatt acctactggc aacgcagatt atcatcactt aaaaagcgaa
     7381 ctgtcagtag accatcggtg agcgtggctc cggcgtagtt ttccagtggt gtgagttttt
     7441 cgcgtatgtc ttccagttgc gtttcattcc ccaggtagaa cagcatcgtt cccacccatg
     7501 gtttccgggc aacgcaggta agttctccat tggccacgtg cagacgctca atcaacagcg
     7561 gagaaccgtc gacccatatc tccagacggt tagcgagtgt tccgtggctg aaggtttctc
     7621 cgatgaccgg acgtccaagg cacagaagat cccaggccag cagtgtactg gaggacgaca
     7681 gatgaaacac ggtatgcaac gtggcgtgag cgccgggaaa aaaaatcgaa tcctgtggca
     7741 accactccag cgttgcgtta ttgcacagcg tcaggttttg ctgaaggtgc gcctgcgcgc
     7801 cgctgctgcg gtaaaattta ctggcaccag gcatggtgat cagcgtatgg caatcagagt
     7861 caattattgc gttgatgatg agttcgtcac cgccgacgat accaccgggt ggatgaagca
     7921 gacagaggtg gcaggtttct tcttcgggat aaaaaggacg ctgaacagtg agcagaccga
     7981 catgatgacg ggtggtgagc gtggttttcc cgtgcagacg ctgaaaacgg agatccagca
     8041 tggcctgcca gcctttttta ttttgtggta acgcggtact gagcaatctt acgcccctga
     8101 taaataaaga taaaacgaca gtaaagagcc ggatttcctt gttatataaa agatgatgcc
     8161 tttccagtga gttttcgtct gaataatacg aatgtttgtt ttcgtcatga agcgttccgg
     8221 ctcccggggc tatacatcgc cagaaaagca atgcatattg tcgggattat ccagagaatc
     8281 aggcagaaag atccgacaat gggctgtgat tatgccatct ggctcagaat gatatcaagg
     8341 tgttatgggg ataacttgct gatacaatag cagtatgaca gattcattaa tgcggaaact
     8401 gctcactgaa acagagtgtt catgactgag cactggtcgg gtcatgcaga tgataatttc
     8461 cccttttctt ggggaagcga cagtattttc atgcatgcct gtcttcagtc atacggggag
     8521 agcatctgtg atacagtcca tacatttgtt gcggacgtcg ctcctgagtc actgactggt
     8581 ctgcaaacag tttatctgta cacggcaggg cttctgtgtg atatcgtttt tcatatcgag
     8641 ggggggcctc cagagatgaa tggaataata a
//
LOCUS       DAZGXS010000165         8656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGXS010000165 DAZGXS010000000
VERSION     DAZGXS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(374..1069)
                     /locus_tag="ACHKG5_004194"
     CDS             complement(374..1069)
                     /locus_tag="ACHKG5_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0870183.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            1144..1359
                     /locus_tag="ACHKG5_004195"
     CDS             1144..1359
                     /locus_tag="ACHKG5_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870184.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            1501..1797
                     /locus_tag="ACHKG5_004196"
     CDS             1501..1797
                     /locus_tag="ACHKG5_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP0870185.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            1830..1991
                     /locus_tag="ACHKG5_004197"
     CDS             1830..1991
                     /locus_tag="ACHKG5_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870186.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            1978..2799
                     /locus_tag="ACHKG5_004198"
     CDS             1978..2799
                     /locus_tag="ACHKG5_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0870187.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNAKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            2796..4172
                     /locus_tag="ACHKG5_004199"
     CDS             2796..4172
                     /locus_tag="ACHKG5_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0870188.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            4243..4521
                     /locus_tag="ACHKG5_004200"
     CDS             4243..4521
                     /locus_tag="ACHKG5_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870189.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            4654..4869
                     /locus_tag="ACHKG5_004201"
     CDS             4654..4869
                     /locus_tag="ACHKG5_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870190.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDFK"
     gene            4880..5116
                     /locus_tag="ACHKG5_004202"
     CDS             4880..5116
                     /locus_tag="ACHKG5_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFP0870191.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            5073..5519
                     /locus_tag="ACHKG5_004203"
     CDS             5073..5519
                     /locus_tag="ACHKG5_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP0870192.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            5516..6043
                     /locus_tag="ACHKG5_004204"
     CDS             5516..6043
                     /locus_tag="ACHKG5_004204"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0870193.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            6040..6222
                     /locus_tag="ACHKG5_004205"
     CDS             6040..6222
                     /locus_tag="ACHKG5_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0870194.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            6497..7201
                     /locus_tag="ACHKG5_004206"
     CDS             6497..7201
                     /locus_tag="ACHKG5_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0870195.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGAEVKNIQNNYARN
                     AERFIEGKHFFKVAGDALKNLRVALNYSQNLQPSLRGLQISPKARSLILWTERGAARH
                     AKMLETDQAWEVFEKLEDCYFSQCKKNTGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WVRLKNKELPPSLHRY"
     gene            7276..7999
                     /locus_tag="ACHKG5_004207"
                     /pseudo
     CDS             7276..7999
                     /locus_tag="ACHKG5_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
     gene            7999..8604
                     /locus_tag="ACHKG5_004208"
     CDS             7999..8604
                     /locus_tag="ACHKG5_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0870196.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTAEECKTIKAEYQQKLKKLRNSRSEAA"
BASE COUNT         2485 a         1975 c         2201 g         1995 t
ORIGIN      
        1 ttcatctcct aaatctcttt tctcttacct aaagaaacag caggctgggt cagccctaac
       61 aacttcaagt gcatcggtca gagaaagctc cgtgctatac aggtgttatt tcatatcttt
      121 ttgcatccaa taaattttcc atctgtccag agagcggatg cacttgattc ttgttgatgg
      181 caggacgttt tttcacctgc ctcaccctgc catacaggac gctgctcacc gatatctatc
      241 gtccggtcat tgatgttata gacaatatcc agatcattac ggatatactc agatggcctt
      301 atgctttcaa tgaatagatg aacttctttt tgaccgcttg atactcatgg ccattaaact
      361 ccatctatcc tctttaccca aacgtctctt caggccattg gctggcgata actttcccca
      421 caacggaaca actctcattg catgggatca ttggatattg cgggtttagt ggttgtagaa
      481 acacctgacc gctatccctg atcagtttct tgaaggtaaa ttcatcacca ccaagtctgg
      541 ctatgcagaa atcgccgggc tcaacagctt gctcagggtc aaccagaatt aacatcccgt
      601 caggaaaact aggtttggaa cctgttggtg cggtcattga gttaccttca acctcaagcc
      661 agaatgcaga gtcactggct tttttggttg tgcttaccaa tctctccgca tcacctttgg
      721 taaaggttct gagttctgga gagaacatcc cagcctgaac atgagaaaaa acagggtact
      781 catattgttt tttaactggg gccgatgagt attcgccaac aggtgaaaat gtcccgtcgt
      841 ggttgaatga tatgttatca ataccaaggt atttaaacac cacaccaata tcactaagag
      901 atggatgacg agatccgcgc aaccagtgtc caattccacc ctgcgtcata cctagctctt
      961 cggctaactt ctcttgagtt atgccgagct ctttcattct ggatctagcc agttcatacc
     1021 atttcatttt catgtcctta ttattacgct ctgtactgga accatccatg cacaatgtgt
     1081 atttttactt gtatttgaga agtacatatt gtatttttta ttcgtggtta ctatggaggg
     1141 catatgagca acctacgaaa atatcgagag tcactgaata tctctcaaac aacacttgct
     1201 aaggcagttg gatgcacaca gggagctatc ggacattggg aatctggtcg tcgcttccca
     1261 gaccttaaaa catgccgtgc tcttgttgcg tgtctaaaca agttaggcgc aaaagtaagt
     1321 cttgatgacg tgttcccgcc ggagcacaaa gccgcttaat aagcggagcc gctctttgta
     1381 acaacggaca ttcgtcctac gtcgctgaaa agcgagtccc aagatatctg accaactaag
     1441 gccatatgcg tttccacgca tacctttcaa ctaactattc actattggaa atcttaagaa
     1501 atggaacgaa caagttacag caaactatca cagcgcgacg ttgatcgcgc agaaacagat
     1561 ttactcatca acctgtcaac gcttacacag cgcggtctgg caaagatgat tggctgtcat
     1621 gaatcgaaga taagcagaac ggactggcgg tttattgctt cggtcttgtg tgctttcgga
     1681 atggcatcag acatcagtcc gattagcagg gcttttaagt atgcgcttga tgaaatcaca
     1741 aagaaaaaat ccccggctgc caccgaggat tttaagcaaa ttgatatgca attctgaggg
     1801 aattactgga tcaatccaca ggagtaatta tgacaaaacg tcgtaagaaa taccaggaaa
     1861 aagaagagat tcgacaccct gattcacctg agggattagt ggtagccgca gcaaataaca
     1921 gggcgttcgc agagcgcctt gttggtgttt acagactagc caaagcagga gtgaaacatg
     1981 ggcgtcgtta agttagctga ttacaggcct caactggagg tcgtggagca tcgcgtggca
     2041 gataccgaag atggtttcat gcgcgttgct aacgagatta ccgacagtct gctgatggct
     2101 gatttaaccg tccggcagtt gaaggtgatg ctcgctatca tgcgcaagac atacggattc
     2161 aataagccga tggatcgact cacaaacacg cagatagcag ccatgacagg tattcatcac
     2221 actcatgttt gcgctgccaa gcgccagctt attgagcgta aattcctcat tgctgatggc
     2281 gtgaaaatcg gagtgaacaa ggtggtttct cagtggatta gccaggacag cttaacatta
     2341 gctaaaacag ctaataaaac attagccaag tcggctaatg ggtataagcc aagtcagcta
     2401 aacgcaaaag acaatataca aaagacaata aatacaaata cccccttacc ccctaacggg
     2461 gatggcgatg ggcaggttaa acctgaacgt cgcaaggcag aacgaatcga ctacgaatcc
     2521 ttcctgaacg cctacaacac cgaagtcggt gacagacttc cacacgctgt tgcggtcaac
     2581 gagaaacgaa aacgccgcct gaagaaaatt atcccgcaac tgaaaacgcc aaacgtggac
     2641 ggtttcagag cgtatgtcag ggcgtttgtg catcaggcca agccgtttta cttcggagac
     2701 aacgacacgg gctggacggc agattttgat tacctgctga gggaagattc gttaacggga
     2761 gttcgggaag ggaagtttgc agacaggggg attgcatgag acaggatatc gaagctagcg
     2821 ttatcggtgg cctgctgatt ggtggattaa ctccaaccgc cagtgacgtt ctggcaacgc
     2881 tggagccgga agcgttttca attccgctct accggaaagc cttcgaggtt attcgcaagc
     2941 aggcgagaaa cagaaaccta atcgatgcgc tgatggttgc cgaggcgtgc ggagaggagc
     3001 atttcacgtc aatcctgatg accagcaaaa actgcccgag tgccgcaaac ctgaagggat
     3061 atgccggaat ggtcgcggat aactatcacc gccgtctggt gctggaaatc atggatgaaa
     3121 tgcgtgaacc aatccaaagc ggaaccatcg acgcatcgag tcaggcgatg gatgaacttg
     3181 taaagcgtct ttcagccatc agaaagcccc gtgacgaggt taaacctgta cggttagggg
     3241 aaatcatcac tgactacact gacacgcttg acaggcgtct gaggaacgga gaagagtcag
     3301 ataccctgaa gaccggaatc gaagagcttg acgctatcac tggagggatg aacgcagaag
     3361 acctggtgat tatcgccgct cgtcctggta tggggaaaac cgaactggcg ctgaagattg
     3421 ccgaaggtgt ggcaagccgc gttattcctg gttctgacgt ccggcgcggg gtattgattt
     3481 tctcaatgga aatgagcgca ttgcagattg cagagcgaag cattgccaac gccgggagga
     3541 tgtcggttag cgtactgcga aatcctgcat cgatggatga cgagggctgg gcacgtgttg
     3601 ctaacggcat gagtcagctt gcagatttgg atgtatgggt agtcgatgct tcgcggttat
     3661 cggtcgaaga aatacgctca atcgcagaac ggcataagca ggaaaatcca aacctgtcac
     3721 tcatcatggc ggattatctt ggcctgattg agaagccgaa agcagaccgc aacgacctcg
     3781 caattgctca catctccgga agcctgaagg cgatggcgaa agacctgaaa acaccggtta
     3841 tctccctgag tcagctttcg cgcgatgttg agaagcgacc aaacaaacgc ccgacaaacg
     3901 cagatttgcg tgattcagga agcattgaac aggacgcaga ctcaatcatc atgctctatc
     3961 gggaagcggt atatgatgag aacagtagcg ccgcgccatt tgctgaaatc atcgtgacga
     4021 aaaaccgttt tggctcgctt ggtacagttt accagcggtt ctgtaacgga cactttgttg
     4081 catgtgacca ggatgaagcc agacagattt gcacagcatc aaatgcacct gctgcgcgtg
     4141 gcagacgata tgcacaaggg gctgacgtat gaccatctac atcactgagt taataacagg
     4201 cctgctggta atcgcaggcc tttttatttg ggggagaggg aagtgaacga tagctaccga
     4261 cagtttgaaa actggtggtc aaaagacaaa agccagttca cgggagacga tgaattaaaa
     4321 gagtttgcct gggtgatatg gcaggcatcg cgctctgcta ttgaactgga tatcgactgg
     4381 cccgaatcga atgacgacct ttggaaagat ggtgaagaag gtgcttatgc gatgggttat
     4441 gaggatgggc gtgacaaaac ggtaattgca gtaatgaaag ccatcagggc cgcaggaatc
     4501 aaagaaaaga atttcgatta agcaaatatc acttcaataa atcgctttta aggcatcaca
     4561 atcgctctgt ggtgaggtaa gcacgtgcaa ggtataccga taagcagcga gaatgaaaaa
     4621 tgcgtcagaa tgcgtttgag gaggttttaa gaaatgagta cgatagctga gcttgtcagg
     4681 gctaattttc gtgaagagtt ggtgcgttgg tatcggtatc gttcatcgtc cagtttgccg
     4741 cttgatgagt tgtatgagca ttcacctgcc gcacgacgct atccgcgtga ccgtgttctt
     4801 cgacggttgt tcaaactcaa caatgagttt cagcgcaaca gaattatccg gagtctggat
     4861 tttaagtgaa ggagtgagca tgagcgacct atcattaacc cagccaaagc taaaagaatg
     4921 tccgttttgc ggcggtaatg ctcgtctgtg ggttgaggcc ggaataaata ttgatgtgtg
     4981 gggctatgca gaatgtgacc tctgtgaagc cagggtggca tgggcaccat cagttgctgc
     5041 ggctactgaa aaatggaacc ggagagcagg agatgaagca aacctttctg cttcgcaacg
     5101 aagcaatcag aaataacgcc atagacgcca ttctctcact acccatcgac gacaagtcac
     5161 cccacgaagt ccacgttaaa gaacccaagc gcagcaaagc gcagaatgac cgcatgtggc
     5221 cgatgctgaa cgatgtttcg cgtcaggtgc tatggcatgg tcaacggctg gcgccggaag
     5281 actggaaaga cctgttcact gccctgtggc ttaagaccaa aaaactggag caaagaagtg
     5341 cgcctggtat cgacggtggc gttgtcatgc ttggcgtgcg taccagcaaa atgcgaaagg
     5401 ccagcatgac tgagcttatc gaaatcatgt tctggttcgg ctcagagcgc aacgtgcggt
     5461 ggagtgatga ctcccggcga gagtatgaat ggtcacaacg aaaagggaag gctgcatgac
     5521 tatcaaatca aatacgccag cacacgacaa ggactgctgg caaacgccgc tctggctttt
     5581 tgatgcgctg gatattgagt ttggattctg gctggattca gctgcgagcg acaaaaacgc
     5641 tctgtgcgct cactggctaa ctgaggctga cgacgcgcta aattctgagt ggataagcca
     5701 cggtgcaatc tggaataacc caccgtacag caatatcagg ccgtgggtgg aaaaagccgc
     5761 tgagcagtgc atacaacagc gacagacggt agtgatgctt gtgccagagg atatgtctgt
     5821 cggatggttc agcaaggctc tggagagtgt tgacgaagtt cgcatcatca ctgatggacg
     5881 gattaatttt atcgaaccat cgacagggct ggagaagaag ggaaacagca aaggctcaat
     5941 gttgctgatt tggcgaccgt tcatcagtcc tcgacggatg tttactaccg tatccaaagc
     6001 ggcattgatg gcgatcgggc tgggcgtcag gagggctgca tgaggcgaca gcgacgaagt
     6061 atcaccgaca tcatctgcga aaactgcaaa taccttccaa cgaaacgctc cagaaataaa
     6121 cgcaagccaa tcccaaaaga atctgacgta aaaaccttca attacacggc tcacctgtgg
     6181 gatatccggt ggctaagaca tcgtgcgagg aaaacaaggt gattgacgtg atgatttatt
     6241 cggggctata ttcctcacgc gccagcaaaa tctggcgtcg ggattggcgt cctggataga
     6301 gaccgcgaca gatacacgcc gcgagcgtgt tttttattgt cgtatgcacg cgcacatctg
     6361 aattatggtg ggctgtgtgg gggcggagag atccgcgccg gtcggtttcc cggttacgcc
     6421 aaccctgcac agttcaccac cagaagattg gcgtcgtcgg tggtgagtta ttaagaaacc
     6481 actcgagggc gtcattatga caactcaaat ctctgttgaa actctctccc cgatcaccca
     6541 taaccagatt cctgttatta ccaccgaact tttggcgcag ttgtacggtg ctgaagttaa
     6601 gaacattcaa aataactacg ccagaaatgc tgagcgcttt attgaaggaa agcatttttt
     6661 caaagtggct ggcgatgccc tgaaaaattt gcgggtagct ttaaactact cacaaaattt
     6721 gcaaccatct ttaagagggt tacaaatttc cccgaaagcc cgctccctca tcctctggac
     6781 agaacgaggc gcagcccgtc atgcaaaaat gctcgaaacc gatcaggcgt gggaggtgtt
     6841 cgaaaaactg gaagactgct atttcagcca gtgcaagaaa aatactggca aacaagagaa
     6901 gagcaccaac gagctttccg caaaagaagc aaacagcctt gtatggttat gggattatgc
     6961 taaccgctca caggcattat tccgcgaact gtatccggcg ctaaaacaaa ttcaatcgaa
     7021 ctattccggc agatgctacg actacggtca tgagttttcg tatgttatcg gaatggcgag
     7081 agatgtttta atcaaccaca cacgagatgt tgatattaat gagccagacg gaccaacgaa
     7141 cctttccgca tgggtaaggc ttaagaacaa agagttgccg ccttcactgc atcgctactg
     7201 acaattgaca acttaacaaa cccagcttcg gctgggtttt ttattactga attttcaatg
     7261 tgagaggaca tgacaatgaa tgagctgata aatagcagta tcatcaaaat gacaagcatt
     7321 gaaatcgctg agttagtggg aagtcgtcca gataacgtaa aaatatcaat agagcgcctg
     7381 gctaaatctg gagttattca acttcctgca ttgcaggttt tcgaaaaaaa tcaataactt
     7441 agggctgcgc cgtagtgtcg aggcttacgt cttcgaaggc gaacaaggta agcgcgacag
     7501 cattattgtc gttgcccagt tgtcgccgga attcaccgct cgtcttgttg accgttggcg
     7561 agaactcgaa ggggcaaccg caaagatccc acaaaccttt tctgaggcat tgcgccttgc
     7621 agctgacctt gaagacaaga aggctgaact ggagaaacag cttgctctcg cagcacctaa
     7681 agttgagttt gccgatcgcg ttggcgaggc cagcggaatt ttgattggaa actatgcaaa
     7741 ggttgttggt attggtccaa acaaactgtt tgcgtggatg cgcgatcaca aaatccttat
     7801 tgcttcaggt gcccggcgca atgtgccaat gcaggaatat atggatcgcg gctatttcac
     7861 agtgaaagaa acagcggtca atacaaatca cggaatacag atatcgttca ccacaaaaat
     7921 caccggtcgt ggtcaacagt ggctgacaag aaagctgcta gataacggaa tgcttaaagt
     7981 aacaggggag gctgcttaat ggctaaacca gcgcgaagga aatgcaaaat ctgtaaggaa
     8041 tggtttcacc cggcattctc aaatcagtgg tggtgcagcc cggaacacgg aactaaatta
     8101 gcgctcgaac gacgaaataa agaacgcgaa aaggcggaaa aaacagcaga gaagaaacga
     8161 cgacgagagg agcagaaaca gaaagataaa cttaagattc gaaaactcgc cttaaagccc
     8221 cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag agaaagagac
     8281 cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga tgccgggcat
     8341 taccggacaa ctgctgcggc gcctcaactc cgatttgatg aacgcaatat tcacaagcaa
     8401 tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt cgaactgatt
     8461 aaccgcatcg ggcaggaagc agtagacgaa atcgaatcaa accataaccg ccatcgctgg
     8521 actgccgaag agtgcaagac catcaaggcg gagtatcaac agaaacttaa aaaactgcga
     8581 aacagcagaa gtgaggctgc atgaatatct acgaaagaat tgatggcagc aaataccgaa
     8641 atatttgggt agttgg
//
LOCUS       DAZGXS010000166         8652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGXS010000166 DAZGXS010000000
VERSION     DAZGXS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1230
                     /gene="lacY"
                     /locus_tag="ACHKG5_004209"
     CDS             <1..1230
                     /gene="lacY"
                     /locus_tag="ACHKG5_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0870197.1"
                     /translation="FWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAI
                     SLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVG
                     GIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQF
                     VFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLS
                     LYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIIN
                     RIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFE
                     VRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTL
                     SGPGPLSLLRRQVNEVA"
     gene            1296..1907
                     /gene="lacA"
                     /locus_tag="ACHKG5_004210"
     CDS             1296..1907
                     /gene="lacA"
                     /locus_tag="ACHKG5_004210"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP0870198.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(2010..3191)
                     /gene="cynX"
                     /locus_tag="ACHKG5_004211"
     CDS             complement(2010..3191)
                     /gene="cynX"
                     /locus_tag="ACHKG5_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0870199.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(3197..3667)
                     /gene="cynS"
                     /locus_tag="ACHKG5_004212"
     CDS             complement(3197..3667)
                     /gene="cynS"
                     /locus_tag="ACHKG5_004212"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0870200.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(3698..4357)
                     /gene="cynT"
                     /locus_tag="ACHKG5_004213"
     CDS             complement(3698..4357)
                     /gene="cynT"
                     /locus_tag="ACHKG5_004213"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0870201.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4467..5366
                     /gene="cynR"
                     /locus_tag="ACHKG5_004214"
     CDS             4467..5366
                     /gene="cynR"
                     /locus_tag="ACHKG5_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0870202.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(5499..6782)
                     /gene="codA"
                     /locus_tag="ACHKG5_004215"
     CDS             complement(5499..6782)
                     /gene="codA"
                     /locus_tag="ACHKG5_004215"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0870203.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(6772..8031)
                     /gene="codB"
                     /locus_tag="ACHKG5_004216"
     CDS             complement(6772..8031)
                     /gene="codB"
                     /locus_tag="ACHKG5_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0870204.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         2103 a         2309 c         2242 g         1998 t
ORIGIN      
        1 ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggagc ctacttcccg
       61 tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt
      121 tttgctgcta tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac
      181 aaactcgggc tgcgcaaata cctgctgtgg attattaccg gcatgttagt gatgtttgcg
      241 ccgttcttta tttttatctt cgggccactg ttacaataca acattttagt aggatcgatt
      301 gttggtggta tttatcttgg cttttgtttt aacgccggtg cgcccgcagt agaggcattt
      361 atcgagaaag tcagccgtcg cagtaatttc gaatttggtc gcgcgcggat gtttggctgt
      421 gttggctggg cgctgtgtgc ctcgattgtc ggcatcatgt tcaccatcaa taatcagttc
      481 gttttctggc tgggttctgg ctgtgcactc atcctcgcca ttttactctt tttcgccaaa
      541 acggatgcgc cctcttccgc cacggttgcc aatgcggtag gtgccaacca ttcggcattt
      601 agccttaaac tggcgctgga actgttcaga cagccaaaac tgtggttttt gtcactgtat
      661 gttattggcg tttcctgcac ctacgatgtt tttgaccaac agtttgctaa tttctttact
      721 tctttctttg ccaccggtga acagggtacg cgggtatttg gctacgtaac gacaatgggc
      781 gaattactta acgcctcaat tatgttcttt gcgccactga tcattaatcg catcggtggg
      841 aaaaatgccc tgctgctggc tggcactatt atgtctgtac gtattattgg ctcatcgttc
      901 gccacctcag cgctggaagt ggttattctg aaaacgctgc atatgtttga agtaccgttc
      961 ctgctggtgg gctgctttaa atatattacc agccagtttg aagtgcgttt ttcagcgacg
     1021 atttatctgg tctgtttctg cttctttaag caactggcga tgatttttat gtctgtacta
     1081 gcgggtaata tgtatgaaag catcggtttc cagggcgctt atctggtgct gggtctggtg
     1141 gcgctgggct tcaccttaat ttccgtgttc acgcttagcg gccccggccc gctttctcta
     1201 ctgcgtcgtc aggtgaatga agtcgcttaa gcaatcaatg tcggatgcgg cgcgagcgcc
     1261 ttatccgacc aacatatcat aacggaatga tcgcaatgaa catgtcgatg acagaaagaa
     1321 taaaagcagg caagctattt accgatatgt gcgaaggctt acctgaaaaa agacttcgta
     1381 gaaaaacgtt aatgtatgag tttaatcact cgcatccatc agaagttgat aaaagagaaa
     1441 gcctgattaa agaaatgttt gccacggtag gggaaaacgc ctgggtagaa ccccccgtct
     1501 atttctctta cggttccaac atccatatag gccgcaattt ttatgcaaat ttcaatttaa
     1561 ccattgtcga tgactacacg gtaacaatcg gtgataacgt attgattgca cccaacgtta
     1621 ctctttccgt tacgggacac cctgtacacc atgaattgag aaaaaacggt gagatgtact
     1681 cttttccgat aacgattggc aataacgtct ggatcggaag tcatgtggtt attaatccag
     1741 gcgtcaccat tggggataat tctgttattg gcgcgggtag tatcgtcaca aaagacattc
     1801 caccaaacgt cgtggcggct ggcgttcctt gtcgggttat tcgcgaaata aacgaccggg
     1861 ataagcaata ttatttcaga gattacaaag ttgaatcgtc agtttaaatt ataaaaattg
     1921 cctgatacgc ttcgcttatc aggcctacaa attcagtgat ctatgtaggc cgcatccggc
     1981 gtgaacaaag cgcagaaaca agcgtcgcat catgcctctt tgacccacag ctgcggaaaa
     2041 cgtgctggtg caaaacgcag ggttataatc atcagcccta cgacgcacag cgcatgaaac
     2101 gcccagtcca tcaagtaatt gccgctgata ctacgcagca cgccagaaaa ccacggggca
     2161 agcccggcga tgataaaacc gattccctgc ataaacgcca ccagcttgcc agcaatagcc
     2221 ggttgcacag agtgatcgag cgccagcagc aaacagagcg gaaatgcgcc gcccagacct
     2281 aaaccacaca ccatcgccca caataccggc aattgcagcg gcagccagat aaagccgcag
     2341 aaacccacca gttgtaacgc cagcgccagc attaacagtt tgcgccgatc ctgatggcga
     2401 gccatagcag gcatcagcaa agctcctgcg gcttgcccaa gcgtcatcaa tgccagcaag
     2461 gaaccgctgt actgcgcgct ggcaccaatc tcaatataga aggcgggtaa ccaggcaatc
     2521 aggctggcgt aaccaccgtt aatcagaccg aagtaaacac ccagcgtcca cgcgcgagga
     2581 gtgaaaacca cgcgaaccgg aatggttgtt gtcttgtggg aagaggcgac ctcgcgggtg
     2641 ctttgccacc accaggcaaa gagcgcaaca acggcaggca gcgcccacca ggcgagtgtt
     2701 tgataccagg tttcgctatg ttgaactaac cagggtgtca gggcggcacc aagcccaccg
     2761 ccgcccatca gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc
     2821 cgtttaatca ccgaaggcat caccgcctga atgatgccga tccccacccc accgagcagt
     2881 gcgctgctaa gcagcagcgc actttgaggg taaagctcac gcatcaatgc accgacggca
     2941 atcagtaaca aactgatggc gacgctgcga cgttcgctga catgctgatg aagccagctt
     3001 ccggccagcg ccagcccgcc catggtaacc accggcagag cggtcaacag ggcagccacg
     3061 ctaaagctca ttccgcttgc ctggcgcaat tgcggtagca gtggcccgac ggaggtgagc
     3121 agtggtcgca tattaagacc aatcagcacc agaaccagca gcatcgtgaa cgtcttttga
     3181 tggttacgca tggctgtcag aacggtttgg tcggcagata tttaccatct aaggtgatga
     3241 ccgcacgttc gccaccttcc gggtccgcca ctttcttaac gtcgagtttg aagttaatcg
     3301 cgctgataat gccatcgcca aatttctcat gaaccaacgc tttcagggtt gtgccgtaca
     3361 cctgcaacat ttcatagaaa cgatacatcg ttgggtcggt tggaatacgg tcatcaatgc
     3421 agccacgcag tgggatcatc tgtaacagta gaatggcgtc ttcgtcgaga tccagcttcg
     3481 ccccgacctg gcgggcggcg tcggcaggaa gcgcctgctg acccagcaaa gccgcggtca
     3541 caaaggcttc tgccagcccg gtgccgtcgg caatctcggc aaatgagaga tcttttttag
     3601 ctttgctgag caaaatggca tcggcaagat caagacgaat attgcggtta atttgcgact
     3661 gaatcatggt gcatctcctg atggtttaaa aataaggtta cgctgcggtc ggttggcgta
     3721 gcggtatggc acaaacgcga ggattagctg ccagtggcac aaactggcgg gttgcgccgt
     3781 caaacgctgc gatgctgccg ctttcaatgt cgtagaccca gccgtgcagg gcgatccgcc
     3841 cctcttcgag cgccaggcgc accgatggat gagtttgcaa attagccaac tgagcaatga
     3901 cgttttcacg taccatcgcc gcagcttttg acggtaaatc ggaatgcggg cgcgcctcat
     3961 taacgacgcg ggctgaatcg gcataacgca gccagtggga gacggcaggc atatggtcca
     4021 tgcactgaca gctggcaatg gcggtcatcg cgccacagtt ggaatgaccg caaatcacaa
     4081 tgtcagatac ccgaagcgca gcgacggcat actccaccga agcagaaacg ccaccgggtt
     4141 ccggcccgta ggaagggacg atattgcccg cattgcgaat aacgaaaaga tcgccaggct
     4201 cacgttgcgt caccagctca gggaccagac ggctgtcgga gcaggagata aaaagtgtgc
     4261 gcgggctttg ctgtgtcgcc aactgtttaa ataaggcttc ccgcttcgga aatgcctcgc
     4321 gctggaattt aaggaatcca tcaataatct ctttcaccgt taacctctat ctgtctctgg
     4381 aatgagaggc agactactcg ccgattgtca taaggtaaaa gtctcattta tgatgagttc
     4441 tattgaattt acttataggt tggcgaatgc tctctcgaca tatcaattat tttcttgccg
     4501 tggctgaaca tggcagcttc acccgtgccg ccagtgcgtt gcacgtctct cagcctgcgc
     4561 tttcccagca gattcgccag ttagaggaga gtttaggcgt gccgctgttt gaccgtagcg
     4621 ggcgaacgat tcatctcact gatgcaggag aagtctggcg acagtacgcc agccgggcgt
     4681 tacaggaact gggagcgggt aaacgggcga ttcatgatgt tgccgatctg acgagaggat
     4741 cgctgcgtat cgccgtcacc cccaccttta cgagctactt tatcggcccc ttaatggcgg
     4801 atttctatgc gcgctacccc ggcatcacgc tccagctaca ggaaatgtcg caggagaaaa
     4861 tcgaagatct gctttgtcgc gacgagctgg acgttgggat tgccttcgcg cctgtgcatt
     4921 tgccggagct ggaggcaatt cctttactga cagaaagttt agcgttagtc gtggcgcaac
     4981 atcatccgct ggctgcctgt gaacaggtgg cgttgagtcg cttgcatgat gaaaaactgg
     5041 tcctgctcag cgcggaattt gccaccagag agcagattga ccactactgc gagaaagcgg
     5101 ggctgcatcc acaggtggtc attgaggcga actcaattag cgcggttctg gagctgattc
     5161 gccgcacttc cctttccaca ttgttaccag cagcgattgc cacacaacat gacgggctta
     5221 aagcgattcc tcttgccccg ccactactgg agagaacggc ggttttgttg cggcggaaaa
     5281 atagctggca gacagccgcc gcgaaggcat ttttgcacat ggcgttggag gaatgtgcgg
     5341 atgttggcga gaatgaatcg cggtaaccac tgacacaatt gcctgatgcg ttacgcttat
     5401 caggcctacg agttcagtgc tctacgtagg cctgataagg cgttcaagcc gcatccggca
     5461 taaactacgc tcgctttgtc aacacacagc ccagccgatc accgtttgta atcgatggct
     5521 tccggctgct ccagatatac ggtggtttgt gccggttgtg tgctggcaat caccttgccg
     5581 ccacgtaccg aataacgtac cggaacctga cggcgcagcg catcaaatcc attttcagcc
     5641 ggcaggataa tcaggttggc gctgtttccg gcggcaatgc tgtaatcctg caaattcaac
     5701 gtcctggcgc tgtggtgggt gattaaattc aggccatcgt taatctgccc atagcccatc
     5761 agctggcaaa catgcagccc catatgcagc acttgcagca tattcgccgt tcccagcgga
     5821 taccacggat cgaagacatc atcgtgacca aagcagacgt taatgccgga ctccagcatc
     5881 tctttaacgc gcgtgatgcc gcgacgtttt ggatacgtat cgaatcgtcc ttgcagatga
     5941 atattgacca gcgggttggc gacaaagtta ataccggaca ttttcagcaa gcggaacaga
     6001 cgtgaggtat acgccccgtt ataagagtgc attgccgtgg tgtggctggc ggtgactcgc
     6061 gcgcccatgc cttcacggtg cgccagggca gcaacggttt cgacaaagcg cgactgctcg
     6121 tcatcgatct catcacagtg aacgtcgatg agacggtcgt atttttgcgc cagggcgaag
     6181 gttttatgca gcgactccac gccgtattca cgggtaaatt caaaatgcgg aatcgccccc
     6241 actacatctg cccctaagcg taacgcctct tccagcaacg cttcaccgtt gggatacgac
     6301 aaaatccctt cctgagggaa ggcgacgatt tgcagatcaa tccacggcgc gacttccagc
     6361 ttcacttcca gcattgcttt cagcgcagtt agcgttgcat ccgaaacatc gacatgggta
     6421 cgcacatgct gaatgccgtt ggcaatctgc catttcagcg tttgccatgc gcgttgtttc
     6481 acatcgtcat gggttaataa cgctttgcgc tcggcccagc gttcaatgcc ttcaaacagc
     6541 gtgccggact gattccagtt cggttgtccg gcggtttgcg tggtgtccag gtgaatatgt
     6601 ggctccacaa acggcggtat aactaaacct tgttcggcat ccaggctgtt ttcagttatg
     6661 ggcatcacgc cggattgcgc atcaatggcg ctgatttttc cgtcctgcag atgaatctgc
     6721 cacagcccct ctttgcctgg caaccgggcg ttaataattg tttgtaaagc gttattcgac
     6781 actgttagcc tccacatgcg tcattgctgc tgtcgtttta cgattcaaaa tcgggttaag
     6841 gatcagatag ctcagcgcgc cacctaatac cgcgttgacc ggaacaattc ccggtaacca
     6901 gtggcctgca gcaatcccca gggcgaccgc cagaatcgcc acccaattga cactcatcat
     6961 acgcgtggtc gcaaagtgct catagcggcg acggttcatc agatagtcgg caatgatcac
     7021 gccacccact ggaggaatag ctgccgaaag gaaggtcaac cagccgacaa aattgttata
     7081 cagccataat gcacagaccg taccgataat accgttgatt accgaaaggg ttttgctcga
     7141 catcccggta atgttggcga aacctaaacc cgacgcatag agcgcgttat cgttggtggt
     7201 ccagatattt agccccagca ccacaatcgc aggcaacagc aggccctgag caatcatcac
     7261 atcagagata tccgccatcc ccagtgccgc agcgcccgct gcaccgaaaa taaacatcaa
     7321 cgagttgccg aggaaaaagg ccaccatcgc caccagcacc gccagtttgg cattgcgacc
     7381 aaagcggaca aagtcagcgg tgagcgtgcc cgcactgata aatgacccca caaccagcgc
     7441 cagcgcgaca ttgaaatcta acggttgtgc gggaacgacc gcttttaagg cgtccaggcc
     7501 gcccatgccg ttaacggcca gccacacgga ataaccgcca agacaggcga tagccggaac
     7561 cgcaatcaac gaaagaaccg tcagcgccga aatgccaaag aagacggtga cggtcatcag
     7621 taaaccggaa acggcaatca gcaaattaat atccagcccg gttgccttgc ccaccggaat
     7681 agcaaacatc gccacaccga cgccaaacca gccaacctgg gtgccgccca gcagcagtga
     7741 aggcagccat gagcctttaa caccaaacga gaagcgagca agaagatgag tggtcaggcc
     7801 ggtttttgcg ccaatgtaac cgagaaatga agtgtaaata ccgaggagaa gattaccgat
     7861 gagaactgcg aggaagaaat catgatagct aagaccggtt ccgagagtgc cgccggtcca
     7921 catactggcg gaaaagaagg ttaatcccag catgacgaac gtcaatgcca ataccccttt
     7981 ccgcgccgac tgcgggaccg gcccctggct aaagttgtta tcttgcgaca cgaaattccc
     8041 cctttttgct gtttgaaacc caaaaaatcc gccgcattct attcatctga agataaaaag
     8101 caatcgtttt cgtggggaaa tatatttttt atatgaatga gtgcaaaaag gtggggggaa
     8161 aattatcggt gtaaaaacag tgggtaggca tgataagacg cgtcagcgtc gcatcaggca
     8221 actacgcacg gtgtcggatg cggcgcgagc gccttatccg acctacggtt caggcctgta
     8281 ggcatgataa gacgcgcctg cgtcgtatca ggcatctgcg cacggtgtcg gatgcggcgc
     8341 gagcgcctta tccgacctac ggttcaggcc tgtaggcatg ataagacgca tcagcgtcgc
     8401 atcaggcact gagcacggtg tcggatgcgg tgcgagcgcc ttatccgacc tacggttcag
     8461 gcctgtaggc atgataagac gcatcagcgt cgcatcaggc atctgcgcat ggtgtcggat
     8521 gcggcgcgag cgccttatcc gacctacggt tcaggcctgt aggcatgata agacgcgcct
     8581 gcgtcgcatc aggcatcggc gcatggtgtc gaatgcggcg cgagcgcctt atccgaccta
     8641 cggttcaggc ct
//
LOCUS       DAZGXS010000167         8590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGXS010000167 DAZGXS010000000
VERSION     DAZGXS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..78
                     /locus_tag="ACHKG5_004217"
     CDS             <1..78
                     /locus_tag="ACHKG5_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870205.1"
                     /translation="RIWVPEGKLYFALMNVLQMFPMKNG"
     gene            203..580
                     /locus_tag="ACHKG5_004218"
     CDS             203..580
                     /locus_tag="ACHKG5_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870206.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            696..1496
                     /gene="ydcF"
                     /locus_tag="ACHKG5_004219"
     CDS             696..1496
                     /gene="ydcF"
                     /locus_tag="ACHKG5_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0870207.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1693..3132
                     /gene="aldA"
                     /locus_tag="ACHKG5_004220"
     CDS             1693..3132
                     /gene="aldA"
                     /locus_tag="ACHKG5_004220"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP0870208.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3174..4175)
                     /gene="gap"
                     /locus_tag="ACHKG5_004221"
     CDS             complement(3174..4175)
                     /gene="gap"
                     /locus_tag="ACHKG5_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0870209.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATSNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4364..4894
                     /gene="cybB"
                     /locus_tag="ACHKG5_004222"
     CDS             4364..4894
                     /gene="cybB"
                     /locus_tag="ACHKG5_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0870210.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5139..5312
                     /locus_tag="ACHKG5_004223"
     CDS             5139..5312
                     /locus_tag="ACHKG5_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870211.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            5338..5406
                     /gene="yncP"
                     /locus_tag="ACHKG5_004224"
     CDS             5338..5406
                     /gene="yncP"
                     /locus_tag="ACHKG5_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0870212.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(5384..5533)
                     /gene="hokB"
                     /locus_tag="ACHKG5_004225"
     CDS             complement(5384..5533)
                     /gene="hokB"
                     /locus_tag="ACHKG5_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0870213.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            5932..7572
                     /gene="trg"
                     /locus_tag="ACHKG5_004226"
     CDS             5932..7572
                     /gene="trg"
                     /locus_tag="ACHKG5_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0870214.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            complement(7610..8533)
                     /gene="ydcI"
                     /locus_tag="ACHKG5_004227"
     CDS             complement(7610..8533)
                     /gene="ydcI"
                     /locus_tag="ACHKG5_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0870215.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         2253 a         1955 c         2163 g         2219 t
ORIGIN      
        1 agaatatggg tgcctgaggg gaaattgtat ttcgcattaa tgaatgtatt gcagatgttt
       61 cccatgaaaa atggttaata actggacaag ataattttaa aattcagcaa cggttatcga
      121 ttatacatgg tacttaaaca gactcacacc atttttgtag aaggttaaat tgagtacagt
      181 ctcatatatt aagggtgtta acatggatga acgttttata aatatcacgc cgacttcgtt
      241 gtcttttaaa cacgccgatc tgtcaggttt aagctataag aatgccaggc ttgaactggc
      301 cctgatatgc ccggcgatca agtcagaagt taagatatca tttgactgga tacactcatt
      361 tcgtgttact gacgaaggtg atttgttagg tatgtcagga aagcagggaa ttaaccaaac
      421 ggggatctat agagtagcca attccactta tcttacatgg tttactatgc aaagttgtga
      481 aattcataaa aataaaatga tcgaacatta tgttattgcc acgtcaaatg atgttattga
      541 tgttttatca acagtttctc cgcaaatagt gtatgactaa aaccagtgtc tggttcgcga
      601 tggcgagata gaaaaagcat tatcttcctt tcagagtgct ccgcttctca ctattatctc
      661 acgcagtatt cttaagggaa cgataaggag gaaccatgaa cattaccccg tttccgacgc
      721 tttcgacggc aactatagat gccataaatg ttatcggaca gtggctggcg caggatgatt
      781 tctccggtga ggtgccgtat caggctgatt gcgtcatact tgcaggaaat gcggttatgc
      841 cgactatcga tgcggcatgt aagattgctc gcgatcagca aattccttta ctgattagtg
      901 gtggtatcgg tcactcgacc acttttttgt atagcgccat cgcacagcat ccgcactaca
      961 acactatccg caccactggc agagcagaag cgaccatcct ggcggatatc gctcatcagt
     1021 tctggcatat tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa
     1081 acgcacgctt tagcatcgcg ctattgaatc aggccgtaga acgagttcac acagctatcg
     1141 ttgtacagga ccccaccatg cagcggcgca cgatggcgac gttccgccgt atgaccgggg
     1201 acaatcccga tgtaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata
     1261 acgcagacag tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc
     1321 tctcactact cactggcgag ctgccgcgtt tacgcgatga tagcgatggc tacggtcccc
     1381 gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga agtcatccat gcatggcaaa
     1441 cgctgaaaca cgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat
     1501 tgcccgtttg tgaaccactt gtttgcaaac gggcataact cctgcctttt atttctgcct
     1561 cttattcctt ttacacttgt ttttatgaag cccttcacag aattgtcctt tcatgatgcc
     1621 gtctctatga tgattgatgt taattaacaa tgtatttacc aaaaacaaac atataaatca
     1681 caggagtcgc ccatgtcagt acccgttcaa catcctatgt atattgatgg acagtttgtt
     1741 acctggcgtg gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc
     1801 cgcattcccg atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca
     1861 caaccagaat gggaagcgtt gcctgcaatt gaacgcgcca gttggttgcg caaaatctcc
     1921 gccgggatcc gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag
     1981 atccagcagc tggctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg
     2041 gagtgggcac ggcgttacga gggcgagatt attcaaagcg atcgtccagg agaaaatatt
     2101 cttctgttta aacgtgcgct tggtgtgact accggcattc tgccgtggaa cttcccgttc
     2161 ttcctcattg cccgcaaaat ggctcccgct cttttgactg gtaataccat tgtcattaaa
     2221 cccagtgaat ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc
     2281 cttccgcgcg gcgtgtttaa ccttgttctg gggcgtggtg aaaccgttgg gcaagaactg
     2341 gcgggtaacc caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag
     2401 atcatggcga ctgcggcgaa aaacatcacc aaagtgtgcc tggaactggg gggtaaagca
     2461 ccagctatcg taatggacga tgccgatctt gaactggcag tcaaagccat cgttgattca
     2521 cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc
     2581 atttatgatc agttcgtcaa tcggctgggt gaagcgatgc aggcggttca atttggtaac
     2641 cccgctgaac gcaacgacat tgcgatgggg ccgttgatta acgccgcggc gctggaaagg
     2701 gtcgagcaaa aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa
     2761 gcggtagagg ggaaaggata ttattatccg ccgacattgc tgctggatgt tcgccaggaa
     2821 atgtcgatta tgcatgagga aacctttggc ccggttctgc cggtagtcgc atttgacacg
     2881 ctggaagatg ctatctcaat ggctaatgac agtgattacg gcctgacctc atcaatctat
     2941 acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg tgaaacttac
     3001 atcaaccgtg aaaacttcga agctatgcaa ggcttccacg ccggatggcg taaatccggt
     3061 attggcggcg cagatggtaa acatggcctg catgaatatc tgcagaccca ggtggtttat
     3121 ttacagtctt aatgagtgaa agaggcggag gttttttcct ccgcctgtgc gcgtcagagt
     3181 ttagcgaatt tttcgagggt gcgaataagc tgcgtgacga agccatattc gttgtcgtac
     3241 caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca
     3301 tcaaacaccg aaccgaaatg gctgccaatg atatcggaag agactatttc ttcatcggta
     3361 taaccaaatg actcgttatt ggaggttgct tgtttaagtg cgttattcac ctcttcggca
     3421 gtcacttttt ttccgagaat cgataccagt tcagtgaccg aacctgtttt caccggcacg
     3481 cgttgcgcat gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt
     3541 gccgcgcccg tagtgtgggg aatgatattt tctgccgcgg cgcgtgaagc acgtaaatct
     3601 ttaccacgcg ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc
     3661 gtgccgactt ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aagacagtta
     3721 gtagtgcatg atgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattgact
     3781 ttataaacga tagttttcat ttcacctgca ggggcggaaa tcaacacctt cttcgcgcca
     3841 gcatcaagat gcgcctgcga tttctcggcg gaggtataaa agccagtaca ttcgacaatg
     3901 atttctgcac ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg
     3961 atacttttcc catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga
     4021 ccatagtttg aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta
     4081 atagcgacaa cgtctatgtt gcttttgact tcaagtaatc gacgcaacac cagtcgaccg
     4141 atacgaccaa aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg
     4201 gatgaaaatt gatcctttcc aggcttagac caggatggcg ggatgggcaa tctccattct
     4261 tacagtgaaa cgtaacgtac tgaaaaaggg tgaacaatat ttaatgaaat tttgagaaaa
     4321 gcccgttatg ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc
     4381 aaggttacaa atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc
     4441 aatggagttt cggggtttct tcccacgtag cgatcggcca ctcatcaaca tgattcatgt
     4501 ttcctgcggc atatcaattc tcgtgctgat ggtcgttcgt cttttgttaa ggctgaaata
     4561 cccaaccccg ccgattatac ctaagccaaa accgatgatg acgggactgg cgcatttggg
     4621 acatttggtg atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta
     4681 taaccgaggc aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa
     4741 tttcgaacgg gtagatagct taaagtcgtg gcatgaaacg ctggcgaatc tgggatattt
     4801 tgtcatcggt ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac
     4861 acttctacgc atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac
     4921 taaaaccgac ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat
     4981 ttatttgtat gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta
     5041 atcaattcac ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt
     5101 caaatcgaac ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt
     5161 tgtttatggg agcgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt
     5221 cgaaaggggg gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta
     5281 ttagtgcatc gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg
     5341 aatctgctag tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg
     5401 ccatgagcgc agcaacctcc ttatcactgt cccggaaccg cagttcgtag agcgtttgtc
     5461 gggtcaggag tgtgaatgtc agaatcgtaa tgcagataat gagcagacac accaccagag
     5521 ggttgcgctt catagccttt ctccttgccg gatggcgggt aagaggctaa catctgaatt
     5581 gcttggttca ttcgttggcc tcggttgata gaaatatcgg tcggggcctt cgtctttctg
     5641 attcccggtt agcctgaaaa cagaaagtct caggcacccg tgagcatcct atgtggtttc
     5701 cttagggacg aaaataatca cctgacgaaa ttgcgtgctg ttttccagaa tttttcgtca
     5761 ttcgggttag ccagtttaac cattcgttac tctcttcatt ccaatggcat taatcttctc
     5821 tgcaataatt gttgtaaaaa tgtgacgcaa agaggctttt agtcgtaagt aattaccgtc
     5881 aaatgccgat gactttctat caggagtaaa cctggacgag agacaacggt aatgaataca
     5941 actccctcac agcgattagg ttttttgcat cacatcaggt tggttccgtt atttgcctgc
     6001 attctaggcg gtatcttagt tctattcgca ttaagttcag ccctggctgg ctatttcctc
     6061 tggcaggccg atcgcgatca gcgtgatgtt actgcggaga ttgagattcg gaccgggtta
     6121 gcgaacagtt cagatttttt gcgttcagcc cggatcaata tgattcaggc cggggctgcg
     6181 agtcgtattg cggaaatgga agcaatgaag cgaaatattg cgcaagccga atcggagatt
     6241 aaacagtcgc agcaaggtta tcgtgcttat cagaatcggc cggtgaaaac acctgctgat
     6301 gaagccctcg acactgaatt aaatcaacgc tttcaggctt atatcacggg tatgcaacct
     6361 atgttgaaat atgccaaaaa tggcatgttt gaagcgatta tcaatcatga aagtgagcag
     6421 atccgaacgc tggataatgc ttataccgat attttgaaca aagccgttaa gatacgtagc
     6481 accagagcca accaactggc ggaactggcc catcagcgca cccgcctggg cgggatgttc
     6541 atgattggtg cgtttgtgct tgccctggtc atgacgctga taacatttat ggtgctacgt
     6601 cggatcgtca ttcgtccact gcaacatgcc gcacaacgga ttgaaaaaat cgctagtggc
     6661 gatctgacga tgaaggatga accggcgggt cgtaatgaaa tcggtcgctt aagtcgtcat
     6721 ttacagcaaa tgcagcattc actggggatg accgtaggga ctgttcgaca gggtgcggaa
     6781 gagatttatc gtggcaccag cgaaatttca gctggcaatg cggacctgtc atctcgcacc
     6841 gaagaacaag cggcggctat cgaacaaact gccgccagca tggagcaact cactgcgacg
     6901 gtgaaacaga atgcggataa cgcgcattat gccagcaaac tggcgcaaga ggcttctatt
     6961 aaagccagcg atggcgggca gacggtttcc ggtgtagtaa aaacgatggg cgctatctcc
     7021 acgagttcga agaaaatttc tgagatcacc gccgtcatca acagtattgc tttccagacg
     7081 aatattctgg cactgaatgc tgccgttgaa gctgcgcgag cgggtgagca agggcgtgga
     7141 tttgccgttg tcgccagcga agtacggaca ctcgcaagcc gcagcgccca ggcggcaaaa
     7201 gaaattgaag gcttgatcag tgaatcagtc aggttaattg acctgggatc ggatgaggtg
     7261 gcaacggccg ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt cacacatatc
     7321 atgcaggaaa ttgccgccgc ctcggatgag caaagtaggg gcataacgca ggttagccag
     7381 gcgatttctg aaatggataa ggtgacgcaa cagaatgctt ctctggtaga agaggcctca
     7441 gcggcggcgg tgtcccttga agaacaggcg gcacgattaa ctgaggcggt ggacgtattc
     7501 cgtctgaaca aacaatctgt gttggcagaa cctcgcggag cgggtgaacc agttagtttc
     7561 gctccggtgt gaaaatgtta aaggagggat cgacagatcc cttcaccttt cagaacggca
     7621 ttgattttcg aatagcgtta atcatcaact ggcaaccaga agagaacgtc gcatctacgc
     7681 gggtcagtat tccaatcggt tcgcctgcac catgtcccgg aacaggcagg gccaccagcg
     7741 tggcatgacg caggtcgtct tttaccgcgc cagaagggac aaaccacacg tagtcgtatt
     7801 caaccgtaag ttgacgagat agcgaagcag acagcgtttc gatacaaccc gaaggaattt
     7861 tacatccctg gctctgtact aatgcatctg aatgctggcg tggcgcagtg ccttctggtg
     7921 atacaacgac cggccattcc agcacccggc ttagcgttac gttctcctga agtagcggat
     7981 gattagggcg gacaaccagc ttcagcgatt caagaaacag cagttcgtaa ttaagcccgg
     8041 tcatcagttc aggatctgac atccgaccaa tgccgatatc gatttccccg gttttcaaac
     8101 ccgccagaat catagggtta ctcattgtcg caacttgcaa ggtcgtctct ttttgttgct
     8161 gatgaaactg acctataacc gaaggtaata tccccagtgc cgcagtaggt agtgcaccaa
     8221 ccctgacgac atcattatta ggaccttctt tacgatgaag cgactgtccg gcagtgttga
     8281 tggcgtcaag tactctgact gcatgcgtta aaaattgttc gccgggtaag gtaagttgcg
     8341 ccccctgacg accacgctca aacaagcgag cgcccgtcag ctgctccagt tcatttaatg
     8401 tcttagagag cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc aaagttcctt
     8461 gttgtgcgac agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga ctaaacagac
     8521 tatttttttc cataagcgat gttaaaaacg aagcggtgtc gctgacaagt gaagttgttt
     8581 gattatgata
//
LOCUS       DAZGXS010000168         8556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGXS010000168 DAZGXS010000000
VERSION     DAZGXS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(182..1375)
                     /gene="tyrB"
                     /locus_tag="ACHKG5_004228"
     CDS             complement(182..1375)
                     /gene="tyrB"
                     /locus_tag="ACHKG5_004228"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0870216.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1628..2707)
                     /gene="alr"
                     /locus_tag="ACHKG5_004229"
     CDS             complement(1628..2707)
                     /gene="alr"
                     /locus_tag="ACHKG5_004229"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0870217.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2760..4175)
                     /gene="dnaB"
                     /locus_tag="ACHKG5_004230"
     CDS             complement(2760..4175)
                     /gene="dnaB"
                     /locus_tag="ACHKG5_004230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0870218.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4258..5241
                     /gene="qorA"
                     /locus_tag="ACHKG5_004231"
     CDS             4258..5241
                     /gene="qorA"
                     /locus_tag="ACHKG5_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0870219.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5407..5649)
                     /gene="pspG"
                     /locus_tag="ACHKG5_004232"
     CDS             complement(5407..5649)
                     /gene="pspG"
                     /locus_tag="ACHKG5_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0870220.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5783..6820)
                     /gene="dusA"
                     /locus_tag="ACHKG5_004233"
     CDS             complement(5783..6820)
                     /gene="dusA"
                     /locus_tag="ACHKG5_004233"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0870221.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            6909..8006
                     /locus_tag="ACHKG5_004234"
     CDS             6909..8006
                     /locus_tag="ACHKG5_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0870222.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8068..8316
                     /locus_tag="ACHKG5_004235"
     CDS             8068..8316
                     /locus_tag="ACHKG5_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0870223.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(8327..8446)
                     /locus_tag="ACHKG5_004236"
                     /pseudo
     CDS             complement(8327..8446)
                     /locus_tag="ACHKG5_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8477..>8556)
                     /locus_tag="ACHKG5_004237"
     CDS             complement(8477..>8556)
                     /locus_tag="ACHKG5_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFP0870224.1"
                     /translation="IAVSMIVGRDNCAFDLDRGNFVLKN"
BASE COUNT         2283 a         2182 c         2056 g         2035 t
ORIGIN      
        1 gaaaaaaaca gggaaaggaa ttccagataa ttgggataaa cttaacaata aaagaactaa
       61 caacgtttat tatctctcac gtagaacgat ggtatcaaaa aaatcatcta taaagaaaga
      121 gtgaaagcga cgacagttta atttcactgc aggctgggta attccagcct gctttcctgc
      181 attacatcac cgcagcaaac gcctttgcca cacgttgcac atttgccgta tttaacccgg
      241 cgacacacat gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt agtcggtcaa
      301 cctgagcggc acttaaaccg gtataactga acatgccgcg ctgattaagc agataatcga
      361 aattgcgttc tggcatctct gtgcttaata ccttcaccag ttcctgacgc attgccagaa
      421 tgcgagtacg catctcttct acttccgcca gccagctggc tttcaatgcc tcgtcattca
      481 gcactgcagc caccacctgc gcaccaaaat tcggcgggct ggagtagttg cggcgaactg
      541 ttgctttcaa ttgccccagt acgcggcctg cagcttcggc atcttcacac agaacagaaa
      601 gtccgccgac gcgctcgccg taaagggaga aaattttcga gaacgaattg ctcaccagag
      661 cgggtaatcc agcgctggca atggcgcgaa tggcgtaggc atcctcttcc ataccggcac
      721 caaatccttg ataggcaata tcaaggaatg ggataagctc gcgggctttg agaatttcaa
      781 tcaccgcatc ccactggtca ttagtgagat cggcacccgt tgggttgtgg caacatggat
      841 gcagcaacac aatactgcgg gcaggtaatg ttttcagcat cgccaacagg tcattaaagc
      901 gcacgccgtt agtcgcttcg tcataccagg ggtaagtgct tacttcgaat ccagccccgg
      961 cgaatattgc tacgtggttt tcccaggtag gatcgctgac ccagacgcct gattccggga
     1021 agtagcgttt caggaaatcc gcgcccactt tcaatgcccc tgagccgcca agggtttgaa
     1081 tggttgctac gcgctgttgt tgcagtaccg gatggtcggc accaaacagc agcggcgcaa
     1141 tggcatggcg atagctgtta agcccttcca tcggtaaata aagcgaagcg ccatgaggca
     1201 ccgcattcag gcgcgcttcc gcctccgcca cggctttcaa ttgtggaata attccgtctt
     1261 cgttgtagta cagaccgata cttaaattca ctttgtcgct gcgagggtct tctttaaaac
     1321 gctccataag cgtaagaatc gggtcgccag cgtaggcgtc aactttttga aacacgcgat
     1381 ggttctccag gtttacgggc aggtggttaa aacacaataa accggaagaa ggcgaagatc
     1441 gagtggatgt tcaggggcga acggcaatta gcaacagagt gagactcatg acaaacgtac
     1501 atccgccaga ggcacgacct ttataagagc atgaaagaat atcaacttat tgaattggta
     1561 ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat caaatattac
     1621 agaacgatta atccacgtat ttcatcgcga cccttgaagt caggcgcgta ataagttcgt
     1681 aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttca ccccacaaaa
     1741 tgaccgggtc cccggcttta tcctgtgcct gtggacctaa gtctacgcag atcatatcca
     1801 tcgccacgcg cccgacaatc ggcacttcgc gaccgttcac cagcactggc gtaccggacg
     1861 gcgcggcgcg cggataacca tcgccatagc ccatcgcaac tacaccaaga cgagtatcac
     1921 gttcgcttat ccaggttcca ccataaccga caggctctcc gactttatgc tcacgcacgg
     1981 caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca ccggtggagc
     2041 gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg
     2101 actgtggcca cagcagaatg ccacccgacg cggcaatgga acgttgacct ggtttgcctt
     2161 cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt ggttcatccg
     2221 cgcgcgcaaa atggctgacg atattcaccg gctgccgaac gtttttgcac tgggtcaggc
     2281 gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga
     2341 gtttcatcca gacggtaacc ggctcgtcca ggctagcctc ttccagcgca gccagctgtt
     2401 cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat
     2461 caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc agtcgcagag
     2521 cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa
     2581 gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg gcaggtgcca
     2641 gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg
     2701 cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata aaataattat
     2761 tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag
     2821 gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg
     2881 ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc
     2941 gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccggacg tttgtcggca
     3001 cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc
     3061 gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt atcggaaagt
     3121 gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca
     3181 cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga ggagtcatcg
     3241 atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg cgcccagtct
     3301 tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag cgacgccaga
     3361 gaacgcatca tgatctgttc tgatggcatc tccagcgaga aaataagtac cggtttatcc
     3421 tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc catcgacgga
     3481 cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatca
     3541 tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct gctgaaataa ctgctcaata
     3601 cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgttcgca
     3661 cgactttcgg caattttaaa gacgcgggat tcagccagat ccagcagatc ttcgctggta
     3721 cgcccctgcg gatcaaaacc agcttcggca atctcattcg caaccgagat catctcacgg
     3781 acaacggcac gttcacgcac gatgtccgca taggaactga tgttcgcagc acttggcgta
     3841 ttttttgaca actctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt
     3901 tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc
     3961 atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat cgtctgctac cacacgctcg
     4021 gctacatcat cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct
     4081 tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggggttca
     4141 gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac cgagataaag
     4201 aatgggtcga aactttacca tatgaagcag accctgacga tacgttctgg aggacacatg
     4261 gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc cgtagagttc
     4321 actcctgccg atccggcgga gaatgaaatc caggtcgaaa ataaagccat cggcatcaat
     4381 tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcactacc cagcggatta
     4441 ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca tattaaggca
     4501 ggcgatcgtg tagtctatgc gcagtcggcg ttaggcgctt acagctctgt gcataacatt
     4561 aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc tgcagcatct
     4621 ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat taaacccgat
     4681 gaacagttcc tgttccacgc agcagctggc ggcgttggct tgattgcctg ccagtgggca
     4741 aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc gcagagcgcg
     4801 ctaaaagcgg gcgcgtggca ggttattaac tatcgtgaag agaatctggt cgagcggtta
     4861 aaagagatca ccggtggtaa aaaagtgcgc gtagtgtacg attccgtggg cagagacacc
     4921 tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtgagttt tggcaactca
     4981 tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc agaaaggctc gttgtatgtg
     5041 acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga ggccagtaat
     5101 gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga gcagcagaaa
     5161 tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgcgc gacgcaaggt
     5221 tccagcctgt tgattccata aaagaaatag ggcttccacc tgggaagccc tttcttttta
     5281 tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa tagtgacaga
     5341 tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga tcatccgcac
     5401 aatcccttag taacgccagc ggtcataacg ctgatatttc ggcacttttg gtgctttaat
     5461 cgccttaata acccacacca ccgcaatcgc caggagtaac cacggcagca acttaatcat
     5521 caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg cggcgataat
     5581 gcccagcaac gaaacgccgg tgaccatcaa catgacaaaa aagccaatca caaaaagtag
     5641 ttccagcatg atgctctccc aaatatgaaa tctcttgcag gcattacaag aatcatgcca
     5701 aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg cgtggtgaat
     5761 ttgactactt tttggtgaaa agttaacgct tatccgccac cagtttgagc gcgtgttcca
     5821 gcacgttaat gtctgcaccc gctttatggg cattttcact taaataacgc cgccactgcc
     5881 gcgcgccagg aataccctgg aacaagccca acatatgccg ggtaatatgg ccaagatacg
     5941 tcccctggct gagttcacgc tcaatgtacg gatacatggc gcgcactacc gccaccggat
     6001 cggcatcggt atccgaggaa ccaaagatct ctcggtctac cgccgccaga atacccggat
     6061 tctgatacgc ttcgcgcccg accatcacgc catccatatg ttgcaaatgc gctttagcct
     6121 cttccagcga cttgatacca ccgttaatcg acatcgtcag atgcggaaag tcacgcttca
     6181 gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc gggcttaacc
     6241 ccgaaagcca ggctttacgt gcgtggatga tgaacatctc acactcgcct ttgccggaaa
     6301 cggtgttgat gaaatcgcag agaaattcat agctgtcctg gtcatcgatg ccaatacgcg
     6361 ttttcaccgt caccggaatc gacaccacat cgcgcatcgc tttcacgcag tcggcaacca
     6421 gctgcgcatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg tcagacgggc
     6481 agccgacatt caggttgatc tcatcatatc cacgcgcttc tgccagcttc gcacactgcg
     6541 ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac cggatgttct tcttcactgt
     6601 acgccaggta atcaccttta ccgtgaataa tcgcccctgt agtcaccatt tcggtataca
     6661 gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc gtccagtcga
     6721 gcattggtgc aacgctaaac cgagaattcc agtaaacacc agttttttca ggcatcacgc
     6781 tggtttgatt aattttttgt gcttcatgat tatcgtgcat ttttgaacat ttcaggctat
     6841 ttttctcgcg ttaggttccc gcacaggttc ccacgtttta tgggaacccg aaataacgag
     6901 gtcgtgtaat ggcgtactat aacatagaga aacgactaaa atccgatggc acaccacgct
     6961 atcgctgtaa tgtgattatc aaagaaaaag gtgttatcac ttacagggaa agcaaaacat
     7021 tccctaaaca tgctcatgcc aaaacatggg gcacacagaa agtgatggaa ttagatctat
     7081 atggcattcc atcatcaaat gcagttgacg gacttacagt ccgtgactta ctacacaaat
     7141 atttaaatga cccaaatgcc ggaggtaaag caggccgtac taaaagatat gtgctggaac
     7201 tgcttatgga tagtgacata tccgcgatca aactatctga actgacagaa aatgacgtaa
     7261 ttgaacattg caggctaaga aacaacgctg gtgcaggccc agcaacagtc agccacgatg
     7321 ttagttatct tggcagtgtt ctggatgcgg caaaacctgt atacggaatc aattacacat
     7381 caaacccggc gaaaagcgct cgtccatatc tacttaaact cggtttgatt ggtaaatcaa
     7441 accgtcgtaa tcgtagacca gcatctgatg aacttgacat gctcattgaa ggccttcaac
     7501 aacgatctac tcataaatgc tcaaaaattc cgttcgttga tatcctcaaa ttttctgtgt
     7561 ggtcctgtat gcgaatcgga gaagtatgcc ggttacgatg ggaagatctc gaccaagaac
     7621 aaaaatctat actagtaaga gataggaaag atccacgtaa aaaggaaggt aaccatatga
     7681 aagttgcctt gcttggggaa gcctgggata tcgtccagcg acaaccaaaa aaatcagaat
     7741 tcatttttcc atataacagc acttctgtta ccgcaggatt tcagagggta agaagcaaat
     7801 taggtattaa agatctgcga tatcatgatt tgcgtagaga aggggcaagt cgcttatttg
     7861 aggctggttt tagtattgag gaagtcgctc aagttacagg acatcgttca ttaaacgtgc
     7921 tatggcaggt atataccgaa ctgtatccga aatctttaca taatcgtttt gaagaactcc
     7981 aaaagagcag aaacaagacc tcttgacact gtttatttat acagataaaa ataacactgt
     8041 atacaaacac agtatagagg gatttttatg cgtattgaaa tctgcatagc caaagagaaa
     8101 atgactaaaa tgccaaacgg tgctgtggat gcgttaaagg aagaattaac tcggcgcatc
     8161 agtaagcgtt atgacgatgt agaagtgatc gtaaaagcca ccagcaatga tgggctttct
     8221 gttacacgca ctgcagataa ggattctgca aaaacatttg ttcaggagac tctgaaagat
     8281 acctgggaat cttctgacga gtggtttgtt cactaataaa cacgtaaatc ggtaacggct
     8341 ggaaatcatt caatactagc actatcgaaa gttcaccagc caaccgcagt acgttcttgc
     8401 atacgaggtg ccgcgtttta catgtcgtac cgagaattaa tttcatttta cgaaaagtta
     8461 acccgccatt gctatcttaa ttttttaaaa cgaagttacc tctgtcaagg tcaaaagcac
     8521 aattatctct tcctacaatc attgaaactg cgattg
//
LOCUS       DAZGXS010000169         8490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGXS010000169 DAZGXS010000000
VERSION     DAZGXS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..1759
                     /gene="espL4"
                     /locus_tag="ACHKG5_004238"
                     /pseudo
     CDS             1..1759
                     /gene="espL4"
                     /locus_tag="ACHKG5_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(1728..3464)
                     /gene="aceK"
                     /locus_tag="ACHKG5_004239"
     CDS             complement(1728..3464)
                     /gene="aceK"
                     /locus_tag="ACHKG5_004239"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0870225.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3648..4952)
                     /gene="aceA"
                     /locus_tag="ACHKG5_004240"
     CDS             complement(3648..4952)
                     /gene="aceA"
                     /locus_tag="ACHKG5_004240"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0870226.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4982..6583)
                     /gene="aceB"
                     /locus_tag="ACHKG5_004241"
     CDS             complement(4982..6583)
                     /gene="aceB"
                     /locus_tag="ACHKG5_004241"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0870227.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6852..7781)
                     /gene="metA"
                     /locus_tag="ACHKG5_004242"
     CDS             complement(6852..7781)
                     /gene="metA"
                     /locus_tag="ACHKG5_004242"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0870228.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7938..8381
                     /locus_tag="ACHKG5_004243"
     CDS             7938..8381
                     /locus_tag="ACHKG5_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0870229.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2158 a         2084 c         2092 g         2156 t
ORIGIN      
        1 gttattttcc ggggcgcccc caaaaccgta tagtatgccg acacattgct gcacaatata
       61 ttaatgatat ttatcagaat gttgactaca aaccccatca agatgattac tcttcagctg
      121 aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca
      181 gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt atgaaggcat
      241 attttgacaa gatggaatca aatgacctca gtgttatggc tgccatatta ttggtagata
      301 accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt atccattacg
      361 tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaatcatg agcgaaagta
      421 aagaggatat taaacactat tcactgatgg attttatgaa tgtagattat agcctcctga
      481 aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca cttccaaaag
      541 aacagctatt gatgctaaaa ggaactgtgg atgaaataac ccctccatta tcacctgcaa
      601 cgatgaattt gctaatggca attggtcaga atcaccaact taagcaactg atgattcaac
      661 tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat agtattaacc
      721 tccccggttt atatttggct ataaattatg gtaatgcgga tatcgttgag actattttta
      781 attcattgtc agagccagga tatgaaggat tactctcgaa aaaaaatctc atgcatattc
      841 tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca cgtaaggata
      901 aaaatgttgt aacctcgatt ctgaacgcct tacctaaact ggccgcaaca catcatttag
      961 ataacgaaca agtgtataaa ttcctgagtg ccaaaaatag tacgtccagc catgttttat
     1021 accatgttat ggcgaatggt gatgccgaca tgctgaaaat tgttttggac gcgttatctt
     1081 tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg aaggcaaagg
     1141 atttttatgg ttgcccagga ctatacctgg cgatgcaaaa tggacatagc gatatcgtga
     1201 aagttattct tgaagcattg cccagcctgg cccaggaaat taacatttca gcttccgata
     1261 ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt atggccatgc
     1321 agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact ctatttaata
     1381 cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata
     1441 cccaggtttg ttttcagcga tacagcataa acagcaaaac gttgtagaga tggtttatct
     1501 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
     1561 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttgtgaat tgggtcaccg
     1621 ggttattgct gaattaatct ttaatacatt aaataagatg gctgaaagct ttggctttac
     1681 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
     1741 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
     1801 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
     1861 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccaatggcg aaactcttcc
     1921 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccggg
     1981 tagcgcggcg gcgggatatc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
     2041 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgccc
     2101 gggaaaatgt tagcagcggc aagctggcga atggcattgc cgtactcttc aatggcgtcg
     2161 cgcagctgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctca
     2221 atataaagat ggcgaatcac aatttgttcg ccgagatcgg tgattttttc cgctgcttcc
     2281 tggagcagta attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca
     2341 aactcctggg tatccgccat tcggcccacg cgatcgtgct ctttcaccag ttgatagcag
     2401 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgacttta
     2461 aatacacggt caaagcccgg cagcgtgaac accaacatca ccatgccacg aatacccggc
     2521 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
     2581 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcggtggt tttacctggc
     2641 agaatttccc gtaaccactc gaccagcgct gcgggcagcg gcgcataaac cataaaataa
     2701 gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcagaca ggtatcaata
     2761 aacagttcgc cgtcatccgt ctggtggatc ggcaataaaa atggcaatgt gccggaaggc
     2821 gttattagtt tgcccaccag ccaggcggct ttattacggt aaaacagttc gttcgccacc
     2881 tgtaaatgac ttttggagag gttttctggc cccaacgttt ccgtcagatg gcgaataatg
     2941 taatggatgt cacggctttt attctgccag tgcagacgca acggcaggtc gctgataacg
     3001 cgcatcagca gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga
     3061 atggtacgaa agcggcgctc tggctgggag ctaaagataa aaagccgctc gggagtaagc
     3121 gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag
     3181 cgcgggtaat ccggcaacag ccgggtgtaa tgctctttga cgcgcagtaa aaattccgcg
     3241 tccgtgcttt ggccgttggt aatgcagcgc agttgctcca cgaccagacc aacgtgatga
     3301 tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcttgttcg
     3361 aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct
     3421 tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag
     3481 gagcagagaa ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt
     3541 gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
     3601 gcgtatatgc cttatccggc ctacagtcag caacgtttgt aggttgctta gaactgcgat
     3661 tcttcggtgg agccagtcag cgcggtaacc gacgacgtgc cgccctgaat aatggtcgtc
     3721 actttatcga agtaacctgt acccacttcc tgctggtgag atacgaaggt gtagccatct
     3781 ttcgcggcgg cgaattccgg ctgctgcact ttctcaacat agtgcttcat accctcgccc
     3841 tgggcatagg cgtttgccag atcaaacatg ttgaaccaca tgctgtggat acctgccagg
     3901 gtgatgaact ggaacttgta gcccatatcc gacagctgct gctggaagct ggcaatagtg
     3961 ttgtcgtcga ggtttttctg ccagttgaac gacggcgagc agttataagc cagcagtttg
     4021 cccggatatt tcgcgtggat agcttccgca aagcgacgcg ccagttccag atccggcgtg
     4081 gaggtttcac accataccag gtcagcatat ggcgcatacg ccaggccacg gctgatcgct
     4141 tgctcaatgc ccgcatgagt acggaagaag ccttcactgg tacgctcgcc ggtaataaat
     4201 tcgctgtcat acgggtcgca atcggaggtg atcagatccg ccgcatcagc atcggtacgt
     4261 gcaaccagca gggttggaac gcccgtcacg tcagctgcca gacgcgccgc gaccagtttc
     4321 tgaatagctt cctgagttgg caccaaaact ttgccgccca tgtgaccgca tttcttcact
     4381 gacgccagct gatcttcgaa gtgaactgcc gctgcaccgg cttcaatcat cgctttcatc
     4441 agttcaaagg cattcaggac accgccaaaa ccggcttccg cgtcggcaac gatcggcagg
     4501 aagtaatcga catagcgcgg atcgcccggc tcaatgcccg cggaccattg gatctgatcc
     4561 gcacggcgga aagtgttgtt gatccgctcc accacagctg gcaccgagtt tgccggatag
     4621 agcgactgat ccggatacat gctggccgcc aggttagcgt ccgccgctac ctgccatccc
     4681 gacagataga ccgcttcaat acccgctttc gcctgttgca gcgcctgacc gccagtcagt
     4741 gcaccgaggc tgttgatgta gccttttttc gactcgccat gcagcagacg ccacattttc
     4801 gccgcgccca gttgcgccag cgtgcattca ggattgactg aaccgcgtaa tttcaccaca
     4861 tcttccgcgc tgtatgggcg agtaattcct tcccaacgcg gttgagtcca ctctttctgt
     4921 aattcttcaa tttgttgtgt acgggttttc atgtgcagat gctccatagt tatgtggtgg
     4981 tttacgctaa caggcggtag cctggcaggg tcaggaaatc aattaactca tcggaagtgg
     5041 tgatctgttc catcaagcgt gcggcatcgt caaaacgccc ctgggagaaa cgttcttcgc
     5101 ccagttcgct ggcaatgact ttcatctctt cgcccagcat ctggcggaac aaggctttag
     5161 ttaccggttt gccattgctc aacgtttttt gatgatggat ccactgccag attgaggtac
     5221 gggaaatttc agccgtcgcc gcatcttcca tcaggccata aatcggcacg cagccgttgc
     5281 cggagatcca tgcttcgatg tactgcacag ccacgcgaat gttggcgcgc ataccttctt
     5341 cggtgcgttc accatcacaa ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt
     5401 cgcgcatcac ttcaagctga tttttacggg agccgagaat gtcgttgaat accgccattg
     5461 ccgtgtccgc aaggcctggg tgagcgatcc atgtgccatc gtgaccgtta ttggcttcca
     5521 gcgatttatc cgcttttact ttgttgagca cctggttatt gtgctcttca tctttgctcg
     5581 gaataaacgc cgccatgccg cccatcgcaa aagcaccgcg tttatggcag gttttaatca
     5641 acaggcgtga gtaagcattc aggaatggtt tatccatcgt tactgcctgt ctgtctggca
     5701 ggacgcgatc gggatagttt ttcaacgttt tgatatagct gaagatgtaa tcccaacgac
     5761 cgcagttcag accaacaata tggtcacgca gcgcgtgaag gatttcatcc atctggaaca
     5821 cggcgggcag cgtttcaatc agcaacgtcg ccttgatggt gccgcgcggc agattaaagc
     5881 gatcttctgc atagctgaag acttcgctcc accaggccgc ttcctgccag gactgggttt
     5941 tcggcagata gaaataggga ccactgccct ttgccaacag tgcctgatag ttgtggaaga
     6001 aatagagcgc aaaatcaaac aggctgccgg ggattgcctc accacgccag gtgacatgtt
     6061 tttccggcaa gtgcagaccg cgtacccgac aaatcaaaac cgctggattg ggcttgagct
     6121 ggtaaatttt gcctgcttca ttggtgtaac tgatggtgcc gttaaccgca tcacgcaggt
     6181 taatttgccc gtcgatcact ttgttccagt ctggtgccag tgaatcttcg aaatcggcca
     6241 taaagacttt cacattggcg ttgagcgcgt tgatcaccat cttgcgctct acggatccgg
     6301 taatctctac gcggcgatct tctaagtccg caggaatccc gcgaattttc cagtcagcat
     6361 cacgaatgga agctgtttcc gaaataaaat caggcaacgt tccgttatca atatcttgct
     6421 gctgctgaat gcgcgctgcc agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct
     6481 cagtcagaaa ttctaccgct tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc
     6541 ttatgaaagc cagttcatcg gttgttgttg cctgttcagt catcgtgcag ctcctcgtca
     6601 tggatccgaa aacttcccca ctgtgaacga aggatcgttg tgcgcttttc gttcagcaca
     6661 actaagacta ctcatttaaa aatcaaaatc aaaaacaatt tccattttaa ttaaaaatac
     6721 ttgataactt attgataaca atatgaataa aatttattca cctactgagg tgtgtttcgg
     6781 aaataaaaaa ggcacccgaa ggtgcctgag gtaaggtgct gaatcgctta acgatcgact
     6841 atcacagaag attaatccag cgttggattc atgtgccgta gatcgtatgg cgtgatctgg
     6901 tagacgtaat agttgagcca gttggtaaac agcaaattac cgtgactacg ccagctcgct
     6961 cgcggtttgt tttgcggatc attgtgcggg aaatagttat acggtacttc cgggtctagt
     7021 ccggcttcca catcgcggaa atattcctgc gccagcgttt gcgcatcata ttcgggatgg
     7081 cccgtcacaa aggcaatgcg cttatcttta ctggcaaaca gatatgcatc cccttcttcc
     7141 gtctctgcca gaatttccag atcggtgtaa tcacgaatca acgctgccgg aaagtcagca
     7201 tagcgcgaat gcggtgccaa gaatgaatca tcaaagccac gcgtcagaag cgcatgagga
     7261 tggagaatat gatgctcgta aacgccagag agtttgtcgg tgcgagtttg cttaggaatg
     7321 ccgtagagga tattgagcgc tgcctgtacc gcccagcaga caaacagcgt cgaggtgacg
     7381 tgatctttcg accactccag cacctgtttg atctgcggcc agtaagcgac atcattaaac
     7441 tccaccaggc ccagcggcgc accggttaca atcagaccgt caaagttctg ttcctgaata
     7501 tcttcaaagt tacagtagaa gttgttcaga tgctctgcgg gcgtgttacg cgattcacgg
     7561 gaatcgatgc gcaacagctg aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga
     7621 aactgatttt cagtttcaat cttcttcggc atcaggttaa ggatcagaac cttaagtgga
     7681 cgaatttcct gaccagacgc acgagaagtt gtcatcacaa agacgttttc ttcacgcaag
     7741 aaattgacgg cgggtagctc gtccggcaca cgaatcggca taacctgatt acctcactac
     7801 atacgcttat acgtttagac atccagatag ctgaagataa ccagaaaatt tgccaatgtc
     7861 gagcctgcat gttgaaggtg agaaagtttc aaggaaaaga ctacgttaga atataagaat
     7921 gactaaaagg agagaaaatg gttattagta ttcgccgctc acggcatgag gaaggggagg
     7981 aactcgttgc gatttggtgt cgatctgtcg atgccaccca cgatttttta tcagcagagt
     8041 atcgggccga gctggaagag ctggttcgtt cctttctgcc ggaagcaccg ttgtgggtcg
     8101 cggttaatga acgggatcag ccggttgggt ttatgttgtt aagtgggcag catatggatg
     8161 cgctgtttat cgatcctgat gtgcgcggct gcggcgtggg ccggatgctg gtgaagcatg
     8221 cgctctcaat ggccccggaa ctgacaacca atgttaatga gcaaaatgag caggcggttg
     8281 ggttctataa gaaggtgggt tttaaggtta cgggacgctc tgaggtggac gatttgggga
     8341 aaccgtatcc gttgctggat ctggcgtatg tgggggaata agctgccccc ctatcgtgcc
     8401 tgcgtaagct ggataaaacg cgccagcgtc gcatccggca tttttttgca ccaacgcaaa
     8461 aaggccatcc gtcaggatgg cctttcgctt
//
LOCUS       DAZGXS010000170         8443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGXS010000170 DAZGXS010000000
VERSION     DAZGXS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..287
                     /locus_tag="ACHKG5_004244"
     CDS             <1..287
                     /locus_tag="ACHKG5_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0870230.1"
                     /translation="ISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRGN
                     AAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            553..2790
                     /gene="nrfB"
                     /locus_tag="ACHKG5_004245"
     CDS             553..2790
                     /gene="nrfB"
                     /locus_tag="ACHKG5_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0870231.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2777..5749
                     /gene="nfrA"
                     /locus_tag="ACHKG5_004246"
     CDS             2777..5749
                     /gene="nfrA"
                     /locus_tag="ACHKG5_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0870232.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5750..6640
                     /locus_tag="ACHKG5_004247"
     CDS             5750..6640
                     /locus_tag="ACHKG5_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0870233.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6823..7584
                     /gene="envY"
                     /locus_tag="ACHKG5_004248"
     CDS             6823..7584
                     /gene="envY"
                     /locus_tag="ACHKG5_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP0870234.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7630..7706)
                     /locus_tag="ACHKG5_004249"
     tRNA            complement(7630..7706)
                     /locus_tag="ACHKG5_004249"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7670..7672),aa:Arg,seq:tct)
     gene            7949..>8443
                     /gene="fimZ"
                     /locus_tag="ACHKG5_004250"
     CDS             7949..>8443
                     /gene="fimZ"
                     /locus_tag="ACHKG5_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP0870235.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGL"
BASE COUNT         2114 a         2164 c         2157 g         2008 t
ORIGIN      
        1 atatcagttc tgctgattta accgcagaga agttcgccac agcaatccga aaccactggc
       61 acgtggagaa taagctgcac tggcgtctgg acgtggtagt gaatgaagac gactgcaaaa
      121 taagaagagg aaatgcagca gaattatttt cagggatacg gcacattgct attaatattt
      181 tgacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa gcagccatgg
      241 acagaaacta tctggcgtca gtctttgcgg ggagcgggct ttcgtaatct tgccctgcct
      301 gctcacctcc aggcacttcc actacatcat gacctgatcg gtgccaccac tcctcttcac
      361 tatgacgtta acctcccctc cttgacacca cgcttttgtc tacattctac gcaaacggca
      421 acttttataa ccagtgtaaa aataacgtgc cgcaatagtc atacctatta atggtaaaaa
      481 gctgtcacaa ttcataaaaa accttaatat acgccaccct aaacataacc agcgttaatg
      541 taaggttttt gtgtggactg gcttcttgat gtttttgcta cctggctcta tggcttaaaa
      601 gtaatcgcga taacgttagc ggtcatcatg ttcatcagcg ggctggacga tttttttatt
      661 gatgtcgtct actgggtacg ccgcattaaa cgcaagttga gtgtttatcg ccgctacccg
      721 cgaatgagtt accgcgaact gtataaacca gatgaaaaac cgttagcgat tatggttccg
      781 gcgtggaatg aaacgggcgt catcggcaat atggccgagc tggcggcgac cacgctcgac
      841 tacgaaaact atcatatctt tgttggcacc taccccaacg acccggacac tcagcgtgat
      901 gttgacgaag tgtgcgctcg cttcccgaat gtgcataagg tagtctgcgc gcgtcctggc
      961 cccaccagca aagccgactg tctgaacaac gtgctggacg ccatcaccca gtttgagcgt
     1021 agcgccaatt tcgcttttgc tggttttatt ctgcatgacg ccgaagatgt gatttcaccg
     1081 atggaattgc gtctgttcaa ctatctggtc gagcgtaaag atctgattca gatcccggtg
     1141 tatccgtttg aacgcgaatg gacgcacttc accagcatga cttacattga tgagttttca
     1201 gagctgcatg gcaaagatgt tccggtgcgt gaagccctcg ccggacaagt gcccagcgca
     1261 ggcgtcggca cctgtttcag ccgccgcgcc gtgaccgctc tgttagctga cggtgacggt
     1321 attgctttcg acgtgcagag tctgactgaa gattacgaca ttggcttccg cctgaaagaa
     1381 aaaggtatga cggaaatttt tgtccgtttt ccggtggtgg acgaagccaa agaacgcgag
     1441 cagcgtaaat ttttacagca cgcacggacg tcaaacatga tctgcgtgcg cgaatatttc
     1501 cccgatacct tttcgactgc ggttcgacaa aaatctcgct ggatcatcgg cattgttttc
     1561 cagggcttta aaacccataa atggacctcc agcctgacgc tgaactactt tctctggcgc
     1621 gaccgcaaag gggcaatcag taactttgtc agcttcctcg caatgctggt gatgatccag
     1681 cttttgctgt tgctggcgta tgaaagtttg tggcccgatg cctggcattt cctttctatt
     1741 tttagcggca gcgcatggtt aatgaccctg ctgtggctaa actttggctt gatggttaac
     1801 cgcatcgtgc agcgggtgat tttcgtcact ggctactacg gcctgacgca ggggctacta
     1861 tctgtcctgc gtcttttctg gggcaacctg attaacttta tggccaactg gcgcgcgtta
     1921 aaacaggtac ttcaacacgg cgatccacgt cgcgtcgcgt gggataaaac aacgcatgac
     1981 ttccccagcg tcactggcga tacccgctcg ttgcgcccgt taggtcaaat tctgctggaa
     2041 aatcaggtca tcactgaaga acagctcgat acagcactgc gtaatcgcgt cgaaggtcta
     2101 cgcctgggcg gttcaatgct gatgcagggg ctgattagcg ccgagcagct ggcacaggcg
     2161 ctggcagagc aaaacggcgt ggcgtgggaa tccatcgatg cctggcagat cccgtcctcg
     2221 ctgattgccg aaatgccggc ctccgtggcg ctgcattatg cggtactgcc gctgcgtctg
     2281 gaaaatgatg agttaattgt cggcagtgaa gatggtattg acccggtttc gctggcggcc
     2341 ctgacgcgta aagtcggacg caaagtgcgt tatgtcattg ttctgcgggg acaaattgtc
     2401 acggggttac gtcactggta tgcacgccga cgcggtcacg atccgcgggc aatgttgtac
     2461 aatgcggttc agcatcagtg gctcacggaa cagcaggccg gtgaaatctg gcggcaatat
     2521 gtgccgcatc agttcctgtt cgccgaaata ctgaccacgc tcggtcatat taatcgttca
     2581 gcaattaacg tgttgttatt gcgccatgaa cgcagttctc tgccgctcgg caagtttttg
     2641 gtcaccgaag gcgttatcag ccaggaaacg ttggatcgcg tcctgacaat tcaacgcgaa
     2701 ttacaagttt cgatgcaatc actattactc aaagcaggtt taaacacaga acaggttgcg
     2761 caactggagt ccgaaaatga aggagaataa ccttaatcgc gtcatcggat ggtctggttt
     2821 actgctgacg tctttattga gtaccagcgc actcgcagac aatatcggca ccagcgcaga
     2881 agagctgggg ctgagcgatt atcgccattt tgttatttat ccccgtctcg ataaggcgct
     2941 gaaggcacag aaaaataacg acgaagcaac cgccatccgc gaatttgaat atatacacca
     3001 gcaggtgccg gataatattc cgctgacttt ataccttgcg gaagcctatc gccattttgg
     3061 tcatgatgac cgggcacggc tgttgcttga ggatcaactg aaacgtcacc caggagatgc
     3121 ccgacttgag cgcagtctgg cggctattcc ggttgaagtg aaaagcatta caactgttga
     3181 agaactgctt gcccagcaaa aagcgtgcga tgctgcgccg accctgcgtt gtcgcagtga
     3241 agtcgggcag aatgccctgc ggctggcaca gttacctgtc gccagagcgc aactgaacga
     3301 cgcgacgttt gctgcatcgc cggaaggaaa aacgctgcga accgatctgc tgcaacgggc
     3361 aatctacctg aaacaatggt cccaggcaga tacgctatac aatgaagcac gccagcagaa
     3421 cacattaagc gcggcagaac gccgtcagtg gtttgacgtg cttcttgccg ggcagctgga
     3481 cgatcggatc ctggcactgc aatcacaggg gatcttcacc gatcctcagt catatattac
     3541 ttacgcgacc gcgctggctt atcgtggcga aaaagcacgc ctccagcatt atctcattga
     3601 aaataagccg ctgtttacca cggacgcaca agagaaaagt tggctctatc tgttatctaa
     3661 atacagcgcc aaccccgttc aggcattggc gaattatacg gtgcagtttg ccgataaccg
     3721 ccagtatgtt gttggcgcga cgctaccggt gctgttaaaa gaaggtcagt acgacgcagc
     3781 gcaaaaactg ctcgccaccc tccccgccaa tgaaatgctt gaggagcgtt atgctgtcag
     3841 cgtggcgacc cgtaacaagg ctgaagctct gcgtctggca cgcttgctgt atcagcaaga
     3901 atcggcaaat cttacccgcc tggatcaact aacctggcaa ctgatgcaga acgagcagtc
     3961 acgggaagct gccgatttat tgctgcaacg ctatcctttc cagggcgatg cgcgtatcag
     4021 ccagacttta atggcgcgac tggcgtctct gctggaaagt catccttacc tggcaacgcc
     4081 ggcgaaggtg gcgattttat cgaaaccctt accgctggcg gagcaacgtc agtggcaaag
     4141 tcagttgccg ggtattgcag ataattgccc ggcaatagtt cgcttgctgg gcgatatgtc
     4201 gccttcctac gatgccgccg cctggaaccg tctggcaaag tgttatcggg acacgctacc
     4261 cggtgtggcg ttgtatgcat ggcttcaggc cgaacaacga caaccgaacg cctggcaaca
     4321 tcgtgcggta gcctatcagg cgtatcaggt tgaggactac gccaccgcac tggcggccag
     4381 gcagaaaatc agtcttcacg acatgagcaa tgaggatctg cttgctgctg ccaataccgc
     4441 ccaggcggca ggaaatggtg cggctcgcga tcgctggcta caacaggcag aacaacgtgg
     4501 actgggaagc aatgccctct actggtggct gcatgcgcaa cgttacattc ctggtcagcc
     4561 ggaactcgca ctgaacgatc tcacgcgctc aatcaatatt gcgccttctg ccaacgctta
     4621 cgttgcgcgg gcgacaattt atcgccaacg tcataatgtc ccggccgcgg tgagtgattt
     4681 gcgcgccgcg ctggaactgg aaccgaataa tagcaacacc caggcagcgc ttggttacgc
     4741 cttgtgggat agcggtgata tcgcacagtc gcgggaaatg ctcgaacagg cgcataaagg
     4801 gctaccggac gatccggcac tgatccgaca actggcctat gtgaaccagc gtctggatga
     4861 catgcctgcg acgcagcact acgcccggct ggtgattgat gacattgata atcaggcgct
     4921 gataacccca ctgaccccag agcaaaatca gcaacgcttc aatttccgcc gtctgcatga
     4981 ggaggtcggt cgccgctgga cgttcagttt cgattcttcc atcggcttgc gttccggggc
     5041 aatgagtacc gctaacaata atgttggcgg cgcagcgcca gggaaaagct atcgtagcta
     5101 cgggcaactg gaagccgaat accgcatcgg acgcaatatg ctgctggaag gcgacctgct
     5161 ctcagtttat agccgcttct ttgccgatac cggagaaaac ggggtgatga tgccggtgaa
     5221 aaatccgatg tccggcaccg gtctgcgctg gaagccgctg cgcgatcaga tctttttcct
     5281 cgccgtcgaa cagcagttgc cgctgaacgg gcaaaatggc gcatccgata ccatgctgcg
     5341 cgccagcgcc tcattcttta atggcggcaa atacagcgac gaatggcacc cgaacggttc
     5401 aggctggttt gcccaaaacc tgtacctcga tgcggcgcaa tatatccgcc aggatattca
     5461 ggcgtggacg gcagattatc gcgtcagctg gcatcagaag gtagctaacg gacagactat
     5521 tgagccttac gctcacgttc aggacaacgg ctatcgtgat aaaggcactc agggcgcgca
     5581 gcttggcggt gtcggggtcc gctggaatat ctggaccggc gagacgcact acgacgcctg
     5641 gccgcacaaa gtcagtctcg gcgtcgagta tcaacatacc tttaaggcga ttaatcaacg
     5701 taacggagag cgcaacaacg cgtttctcac cattggagtg cactggtaaa tgcgtaagtt
     5761 cattttcgta ttgctgacac tgcttttggt cagccctttt tcctttgcga tgaaaggtat
     5821 tatctggcaa ccacaaaacc gtgatagtca ggttaccgat acccagtggc aggggctgat
     5881 gagtcagtta cgtttgcaag gcttcgatac cctggttttg caatggaccc gttacggcga
     5941 tgcattcacc cagccagaac agcgcgcgtt attgtttaag cgggccgcag ctgcgcaaca
     6001 ggctggcctg aagctgattg tcgggctgaa cgccgatccg gaatttttta tgcaccagaa
     6061 acagtcatcc gcggcgctgg aaagctatct taatcgcctg ctggctgccg atctccagca
     6121 agccagatta tggagtgccg tacctggcgt aacgccggat ggctggtact tcagcgctga
     6181 aattgacgac ctgaactggc gcagcgaagc cgcccgtcag cctttgctaa catggttaaa
     6241 caacgcgcag cggctgatta gcgatgtttc agcaaaaccg gtttatatca gtagtttttt
     6301 cgccggaaac atgtcgccag atggctatcg ccaactgctg gaacacgtta aagcaaccgg
     6361 cgttaatgtc tgggtacagg atggcagcgg cgtggataaa cttaccgctg aacagcgtga
     6421 acgttatcta caggccagcg ccgattgcca aagctccgcc ccagcaagcg gcattgttta
     6481 tgaactgttt gtcgccggca aaggcaaaac ctttacagcg aaaccgaaac cggacgcaga
     6541 aattgcctcg ctgttagcga aacgttcctc ttgcggtaaa gacactctct atttctctct
     6601 gcgctatttg cccgtcgcgc acggcattct cgagtattaa atctcctcca ggtaagtcgg
     6661 gtacgacctg gcttacctct ttcgctctcg tcagaattcc atcaagatta attgctaaaa
     6721 agcgtgctaa agaaagaaga tcgttatcgg cgaacgggcc gatcactcat gcaggaagca
     6781 tagatccttt gagcggagtt tctgatatct gaggagtgcg aaatgcaatt gagcagcagt
     6841 gaaccttgcg tggtgatcct gaccgaaaaa gaggtagagg taagcgtcaa taaccatgct
     6901 acgtttaccc ttccgaaaaa ctacctggcc gccttcgcgt gcaacaataa cgtcattgaa
     6961 ctctcaacgt taaatcacgt attaatcacc cacatcaacc gtaacatcat caacgattat
     7021 ctgttgtttt taaataagaa cttaacctgt gtaaagccct ggtcgcggct ggctaccccg
     7081 gttatcgctt gtcatagccg tacaccggaa gtgttccggc tagccgccaa ccacagcaag
     7141 cagcaaccca gcaaaccctg cgaagcggag ttgacgcgcg cattgctttt taccgtattg
     7201 tctaactttc ttgagcaatc gcggtttatt gccctactga tgtatatctt acgcagcagc
     7261 gtccgcgaca gcgtctgccg cattattcaa agcgatattc agcattactg gaatctgcga
     7321 attgtcgcca gttcgctatg tttaagcccc agcctgctca aaaagaaatt aaaaaacgaa
     7381 aataccagct atagccagat tgtcacagag tgtcgtatgc gttacgccgt acagatgtta
     7441 ttgatggata acaaaaatat cactcaggtg gcgcaattat gtggctatag cagcacgtcg
     7501 tactttatct ctgtttttaa ggcgttttac ggcctgacac cgttgaatta tctcgccaaa
     7561 cagcgacaaa aagtgatgtg gtgaagggca aagcggaaac ggataagacg ggcataaata
     7621 aagaagaaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta gaaggtcgtt
     7681 gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt
     7741 atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca agtgaacgac
     7801 cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacgcacac cgctgcgcgt
     7861 ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc ctaacggaaa
     7921 attgtccgct cctatgagac tggtaactat gaaaccaacg tcggtgatca ttatggatac
     7981 tcatcctatc atcagaatgt ctattgaagt tctgttgcaa aaaaacagtg aattgcagat
     8041 tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc gtcctgttga
     8101 tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc tgaaaaggat
     8161 caaacaaatc cagagcacag tgaaagtgtt atttttatca tcgaaatcag aatgctttta
     8221 tgctggcaga gcgatacaag ctggtgctaa cggttttgtc agtaaatgca atgatcagaa
     8281 tgatattttt catgccgttc agatgatcct ctccggatac acgttttttc ccagcgaaac
     8341 gcttaactat ataaaaagca ataaatgtag tacgaatagt tcaacggtca ctgtgctatc
     8401 taatcgtgaa gtgaccatat tacgttatct agttagcgga tta
//
LOCUS       DAZGXS010000171         8361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGXS010000171 DAZGXS010000000
VERSION     DAZGXS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(140..216)
                     /locus_tag="ACHKG5_004251"
     tRNA            complement(140..216)
                     /locus_tag="ACHKG5_004251"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Asp,seq:gtc)
     gene            complement(349..1080)
                     /gene="dnaQ"
                     /locus_tag="ACHKG5_004252"
     CDS             complement(349..1080)
                     /gene="dnaQ"
                     /locus_tag="ACHKG5_004252"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0870236.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1145..1612
                     /gene="rnhA"
                     /locus_tag="ACHKG5_004253"
     CDS             1145..1612
                     /gene="rnhA"
                     /locus_tag="ACHKG5_004253"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0870237.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1609..2331)
                     /locus_tag="ACHKG5_004254"
     CDS             complement(1609..2331)
                     /locus_tag="ACHKG5_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0870238.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2365..3120
                     /gene="gloB"
                     /locus_tag="ACHKG5_004255"
     CDS             2365..3120
                     /gene="gloB"
                     /locus_tag="ACHKG5_004255"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0870239.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3192..4550
                     /gene="mltD"
                     /locus_tag="ACHKG5_004256"
     CDS             3192..4550
                     /gene="mltD"
                     /locus_tag="ACHKG5_004256"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0870240.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4598..5368)
                     /locus_tag="ACHKG5_004257"
     CDS             complement(4598..5368)
                     /locus_tag="ACHKG5_004257"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0870241.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5446..6246)
                     /locus_tag="ACHKG5_004258"
     CDS             complement(5446..6246)
                     /locus_tag="ACHKG5_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0870242.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6487..7401
                     /gene="yafC"
                     /locus_tag="ACHKG5_004259"
     CDS             6487..7401
                     /gene="yafC"
                     /locus_tag="ACHKG5_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0870243.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7398..8201)
                     /gene="dkgB"
                     /locus_tag="ACHKG5_004260"
     CDS             complement(7398..8201)
                     /gene="dkgB"
                     /locus_tag="ACHKG5_004260"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0870244.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2228 a         1998 c         2043 g         2092 t
ORIGIN      
        1 gaatgagaca acaagcgaga atcacgcact tacgccagag taaaatcatt gaacagagaa
       61 atggtggaat tcaaatacaa aaaaaaccgc aaaattaaaa atcttgcggt tctctgaact
      121 cattttcatg aggtgaattt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg
      181 acaggcaggt attctaaccg actgaactac cgctccgcgt tgtgttccgt tgggaacgag
      241 gcgaatatta cggattgcct cacccttcgt caacggtttt tctcatcttt tgaatcattt
      301 gctgcaaaaa tcgcccaagt cgctattttt agcgcctttt acatgtattt atgcccgcca
      361 gaggcagctt ccgccctttt tctgcaccag atcgagacgg gcttcatgtg ctgcaagctc
      421 ttcatctgtc gcaaaaacaa cgcgtaactt acttgcctga cgtacaaggc gctggattgt
      481 tgcttcacct tgttgctgtt gtgtctctcc ttccatcgca aaagccatcg acgtttgacc
      541 accggtcatc gccagataaa cttccgcaag gatctgggca tcgagtaatg ccccgtgcag
      601 cgttcgttta ctgttatcta tttcgtagcg ggcacataac gcatcgaggc tgttgcgttt
      661 accgggaaac attttcctcg ccaccgcaag gctatcggtg accttacaga aggtattagt
      721 cttcggaata tcgcgcttaa gcaacgagaa ctcgtagtcc ataaagccga tatcgaacgc
      781 tgcgttatgg atcaccaact ccgcgccgcg gatatagtcc atgaactcat cggctacttc
      841 ggcaaacgtg ggcttatcga gcaaaaattc atcggcaata ccatgtacgc caaaggcttc
      901 cggatccacc agccgatcgg gtttgagata aacatggaag ttattgcccg tcaggcgacg
      961 gttcaccact tcaacggcac caatctcaat gatcttgtgg ccttcatagt gcgcaccaat
     1021 ctggttcata ccggtggttt cggtatcgag aacgatctgg cgtgtaattg cagtgctcat
     1081 agcggtcatt tatgtcagac ttgtcgtttt acagttcgat tcaattacag gaagtctacc
     1141 agagatgctt aaacaggtag aaattttcac cgatggttcg tgtctgggta atcctggacc
     1201 tgggggttac ggcgctattt tacgctatcg cggacgcgag aaaaccttta gcgcaggcta
     1261 cacccgcacc accaacaacc gtatggagtt gatggccgct attgtcgcgc tggaggcgtt
     1321 aaaagaacat tgcgaagtca ttttgagtac cgacagccag tatgtccgcc agggtatcac
     1381 ccagtggatc cataactgga aaaaacgtgg ctggaaaacc gcagacaaaa aaccagtaaa
     1441 aaatgtcgat ctctggcaac gtcttgatgc tgcattgggg cagcatcaaa tcaaatggga
     1501 atgggttaaa ggccatgccg gacacccgga aaacgaacgc tgtgatgaac tggctcgtgc
     1561 cgcggcgatg aatcccacac tggaagatac gggctaccaa gtcgaagttt aagcctgtgg
     1621 tttacgacat tgccgggtgg ctccaaccgc ctggcgaatt cgtggcttgt ttttactctg
     1681 tttcatcgga tttagcgtta aaggaatagt ccgtttccgg gcaacaataa gttgtaagca
     1741 accaagcgca ggaatatgcg cattcaaaag ttttcctccg tgtttgttcc acgggagaac
     1801 gtggaaacgg ctggcgtgta gcacttcaaa attcaacaaa gagagccagt ccagttgccg
     1861 catcagagta aacatccggc tgttataggg cgaggttttg cgcaataccg gcacaagttt
     1921 gcgtaatccc attaaactaa tgggattgaa gccactaatg accagccagc catcatcaat
     1981 caatacccga tcggcttcac gcaataaacg atgcggatcg gtgcaccacg gcaatgtatg
     2041 tgccagtaga caaacatcaa cggatttatc ggcaaaagga agatgaagtg ggtccgcctg
     2101 cacctggaca ggcattcctt gcgcagaaac attcacttga tgagaaaccg cgcacgcttc
     2161 gcaattgatt tctgcgctta aattgccaat cttaagcaga taaaaaccat acattttagt
     2221 gaaccacggg ttgagctggc gctccagcgc cttgcgataa agctctcccc agggcaaatc
     2281 gccccagcga tcaggagcca cgacagtttg agggactctt gccggtttca tcacaacctt
     2341 ccgtttcaca ctgagaggta atctatgaat cttaacagta ttcccgcctt tgatgacaat
     2401 tacatctggg ttttgaatga tgaagcaggt cgctgcctga ttgtcgatcc cggagacgca
     2461 gagccagtat taaacgccat tgccgccaat aactggcaac cggaggccat atttctcacc
     2521 caccatcatc acgatcacgt tggcggcgta aaagaactgg tggaaaagtt tccgcaaatt
     2581 gtggtgtatg gtccacaaga gacacaagat aagggaacaa cacaggtagt caaagatggc
     2641 gaaactgcct tcgttttggg gcatgaattt agtgtaattg ccacgccggg tcacacttta
     2701 ggacatatct gttacttcag taaaccttat ctattttgcg gcgacacgct gttttctggt
     2761 gggtgtggtc ggttgtttga agggacagca tcacaaatgt atcaatcact taaaaagtta
     2821 agtgcgttac ctgacgatac attggtatgt tgtgctcatg aatatacgtt atcaaatatg
     2881 aagtttgctt tgagtattct tccgcacgat ttgtccataa atgattatta tcgtaaagtt
     2941 aaggagttac gggcaaaaaa tcaaataaca ctacccgtaa ttctgaaaaa tgagcggcaa
     3001 attaatgttt ttttaagaac ggaagatatt gatttaatta atgtaattaa tgaagaaaca
     3061 ttattgcaac aacctgaaga gcgttttgca tggttaaggt caaagaaaga taggttctga
     3121 taaaactttc ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta
     3181 ttgacgcaca catgaaggca aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc
     3241 agagtaccgg caacgttcaa cagcacgcac agagcctttc tgcagctggt caaggggaag
     3301 cagcaaagtt tacaagtcag gcacgatgga tggacgatgg gacgtctatc gcgccagatg
     3361 gtgacttgtg ggctttcatt ggcgacgagc taaagatggg aattccggaa aatgaccgga
     3421 ttcgcgaaca gaaacagaaa tatttacgca ataagagcta tctccacgat gtaactttac
     3481 gggcagagcc gtatatgtac tggatagccg ggcaagttaa aaaacgtaac atgcctatgg
     3541 aactggtact actacccata gtggagagcg cttttgatcc tcacgcaacg tctggcgcca
     3601 atgccgcagg catctggcag atcattccga gcacggggcg caattatggt ttgaaacaga
     3661 cccgcaatta tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg ctgaatatga
     3721 tgcagcgtct gaacaaaatg tttgatggcg actggcttct gaccgtagcg gcttataaca
     3781 gcggcgaagg tcgggtcatg aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg
     3841 acttctggtc gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat
     3901 tgagtgatat tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg accgatgaaa
     3961 gccgtgctct ggcgcgtgtg cacctgagta gcccggttga aatggcgaag gttgcagata
     4021 tggcggggat ttccgtcagc aagctgaaga cattcaacgc tggcgtgaaa ggctccacgc
     4081 tgggcgcaag tggtccgcag tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg
     4141 aatctctggc ttcaggcgaa attgctgctg tacagtcgac gctggttgcc gacaatacgc
     4201 cgcttaacag ccgtgtttac accgtacgct ctggcgacac gctttcaagt atcgcttcac
     4261 gtctcggcgt aagcaccaaa gatttgcagc agtggaacaa actgcgcggc tctaagctga
     4321 aaccaggcca aagtctgacg attggcgcag gtagtagcgc acagcggttg gcaaacaaca
     4381 gcgatagcat tacgtatcgt gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc
     4441 acggcgtgaa catcaaagat gtgatgcgct ggaacagcga tactgcgaat ctgcaaccag
     4501 gcgataagct gacgttgttt gtgaaaaaca acagcatgcc agactcctga caaaccagat
     4561 aatagaaagg caccgattcc cccggtgcct tttttattta tgccgcttta tgtgcatcta
     4621 ccatgatgat atcactggtg aaagagccat cattctgcaa ggcaaaatac gttctcacct
     4681 ctgtcgatgc gctctgttgg taaatgcgaa tagcgtctac taacgcttct ggcgtacgca
     4741 ttctcgcgac ccatgaagaa aattccagcg gtaacttatc tgtaattaaa ttatcaacta
     4801 tcagattggc ttcattgatt aacctcaacc actcaccgct ggcgtagttt cgtacgtgag
     4861 aggtatcgca taatgcttct accgtctgta accagatgtc gcgcactggg tggcccggag
     4921 acattacgtc catcacaatc agcctaccgc caggtttcaa tatccggttc acttcacgca
     4981 gtgctgcacc aacatcatgc cagtgatggg cagaataacg gctgataaca atatcaaatg
     5041 cgttatcggc aaatggcaga ctttcggcat acccctggcg ggtggtgata tttttcagtt
     5101 gccggacttc ggcagcttgt gccacgacat ccagcatttg ggcagataag tcatacgcca
     5161 ccaccgtgct cacattttgc gcagcgacaa agctggcatg ccctgctccg cagcccatat
     5221 caagtacact tgcatcagga taatcagcca gacacaccgc caggagctgt aaatctcgtc
     5281 cggatgcatg tacggtactg gtcagatatt cgctggcttg agagctaaac tgcttttcta
     5341 cgtggtcatg gtgggattgt gttgtcattg cactgtcctt ttgttgtttg agtataaaag
     5401 ggcagctcca aacgttggtc tgccctgtgg cagacctgac ataccttatt tatcaggctt
     5461 gccgggactg aattcaacga gtagcggatt gtgatcggac gcgcgcgtaa ccagtacaga
     5521 agcttcgctg acgttcagac cacggtagaa aacaaaatcg agcgggcgac caaacgcccg
     5581 acggcgctga tcatcggtaa aacgcacctg acgcagcgac atttcccgcg caaaacgata
     5641 taacgcgttc atccttctac ggctccaagc attgaaatct cccgccataa tgaccgggcc
     5701 gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataaa catccacgcc
     5761 cagactgaag ttgacggcgt gtatattaac caccattaac aggcgggtgt caggtaatgg
     5821 atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt cacgtaacgg
     5881 gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat gttgtggcag
     5941 cacgaaagcg ggtacctgat cggcggcaag atagttagca gtcgcaaact gtaccaactc
     6001 tggcgttgtt tgcgcttcct gcaataacac cagatgtgcg tctttgccgt aattctttaa
     6061 taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga tccgaatacg
     6121 ctcttcggta cttaacggtt ctccgggtgg taatgcctgg ccgatgctcg caaaagaccc
     6181 cggcggtaag atcctctccg caggttgtcc ggcaacatag cgcatggcat aggtgttttt
     6241 tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg gagggttcta
     6301 ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt atgtaagcaa
     6361 gtgcttacac cggtcagaat tttgctcatt attgagaaca ttaaatcgga tgacatatca
     6421 cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcatctattt tgcaacagga
     6481 gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt cgaaagcggc
     6541 agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag ccgggcggtg
     6601 aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacgcg gcaacttagc
     6661 ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca ggagatggca
     6721 gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt gcgcatcgat
     6781 gccgcaactc cagtggtgct gcactttctg atgccgttga ttaagccttt tcgtgaacgc
     6841 tatccggaag tcactttatc gctagtctcc tccgaaacga ttattaattt gatcgaaaga
     6901 aaagtggatg tcgcgatacg cgccggtacg ttaacggatt ccagcttacg tgccaggccg
     6961 ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg ctacgggaag
     7021 ccagaaacga tcgacgattt aaagcaacat gtttgcctgg gattcactga acccgcttcc
     7081 ctcaatacct ggccggtagc ctgtagcgat ggacaattac atgaggtgaa gtacggtttg
     7141 tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg gattgcgtgt
     7201 ttgtccgact atatgatcga cagagaaatc gctcgcggag aattggtgga gttaatggca
     7261 gataaagtgt tgccagtgga aatgccattc agtgccgtct attacagcga ccgtgcggta
     7321 agtacgcgca tccgggcttt tatcgatttc cttagcgagc atgtaaaaac agctcccgga
     7381 ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct aaccaggcgg
     7441 tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg taaattttgt
     7501 gccttaagat tactctccag gttttcacgt ttagtagaag aaggaattac tgagtaacct
     7561 tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt agctgcgata
     7621 cgagcaataa cctcatcttt cagggcctta ccatacgcca gcgtcatata ggaagtaata
     7681 tggatgccgt gctgtttagc ccaggcaacc actttacggt tttgcagata aggagagagt
     7741 tcaatctggt tagtagcgat gttttcagcg ccaacagcag caatcgcctt ttccatcaat
     7801 gggatcgtga agttggaaat accgatctca cgcgtcaacc cttctttttt ggcttccagc
     7861 agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca gtggattagc
     7921 gtcagatcaa cataatcggt acgcaatttt tgcaggctct cttttagact cgggatcaat
     7981 ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc atgacgtggc
     8041 acgccacttt ctgcaatcgc cagacctact gcggcttcgt tatcatagat ttgtgcagta
     8101 tcaattgcgc gataaccaag ttcaagcgcc gttttcacag atgaaataac aacgtcgtct
     8161 ttcagacgga aagtacctaa accaaatgca gggatagcca taatatgcct cttttaatgc
     8221 tattttcgtt aactgaggtg attatgggct ttagaatgtt gcggaaaaag ggcgtaaaac
     8281 gcagaggatt tttgcgattt tagcaataat agaaatgtag acgaaaaaaa acctcgagcg
     8341 ataactcgag gcttcttaat a
//
LOCUS       DAZGXS010000172         8353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGXS010000172 DAZGXS010000000
VERSION     DAZGXS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(350..425)
                     /locus_tag="ACHKG5_004261"
     tRNA            complement(350..425)
                     /locus_tag="ACHKG5_004261"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Asn,seq:gtt)
     gene            complement(526..2013)
                     /gene="yeeO"
                     /locus_tag="ACHKG5_004262"
     CDS             complement(526..2013)
                     /gene="yeeO"
                     /locus_tag="ACHKG5_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0870245.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            2174..2249
                     /locus_tag="ACHKG5_004263"
     tRNA            2174..2249
                     /locus_tag="ACHKG5_004263"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2207..2209,aa:Asn,seq:gtt)
     gene            complement(2287..3237)
                     /gene="cbl"
                     /locus_tag="ACHKG5_004264"
     CDS             complement(2287..3237)
                     /gene="cbl"
                     /locus_tag="ACHKG5_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0870246.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3339..4256)
                     /gene="nac"
                     /locus_tag="ACHKG5_004265"
     CDS             complement(3339..4256)
                     /gene="nac"
                     /locus_tag="ACHKG5_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0870247.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4582..4657
                     /locus_tag="ACHKG5_004266"
     tRNA            4582..4657
                     /locus_tag="ACHKG5_004266"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Asn,seq:gtt)
     gene            complement(4713..5648)
                     /gene="ldtA"
                     /locus_tag="ACHKG5_004267"
     CDS             complement(4713..5648)
                     /gene="ldtA"
                     /locus_tag="ACHKG5_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0870248.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5710..6789)
                     /gene="cobT"
                     /locus_tag="ACHKG5_004268"
     CDS             complement(5710..6789)
                     /gene="cobT"
                     /locus_tag="ACHKG5_004268"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0870249.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6801..7544)
                     /gene="cobS"
                     /locus_tag="ACHKG5_004269"
     CDS             complement(6801..7544)
                     /gene="cobS"
                     /locus_tag="ACHKG5_004269"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0870250.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7541..8086)
                     /gene="cobU"
                     /locus_tag="ACHKG5_004270"
     CDS             complement(7541..8086)
                     /gene="cobU"
                     /locus_tag="ACHKG5_004270"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0870251.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8238..>8353)
                     /locus_tag="ACHKG5_004271"
     CDS             complement(8238..>8353)
                     /locus_tag="ACHKG5_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP0870252.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2313 a         2253 c         1801 g         1986 t
ORIGIN      
        1 tccccgttcc cggagaaata atatttatta aaattccagg tcttcttttt ctgattaccg
       61 aagacaaagt ggcaattacg tatagtttcc cgataaagac gcgacagcga catcccgcat
      121 aaggcatttt ttctttatct ttgtacggta cttcatggaa cagagttttt gaccttgcga
      181 atcgtgatgt ctgttggggg caatttactc actgaagcgt gagactcgat taagcgcacg
      241 aaacacagaa atcaaaaaac ccggtcactt tttacaaggc aaccgggtaa aaataatttt
      301 tattttttaa ctgccctgag actcataaag atgcctcaaa actaaaattt ggctcctctg
      361 actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca
      421 gaggaatcgt gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt
      481 ttttaaattt gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacagc
      541 tggctttttt tcacactttt gcggttcggg tcgagggtat ttccacagcc aacgtccagt
      601 aaccattcgc cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca
      661 gacaccaacc acaccccagc caagcatgat gcccagcaca taaccgacga caacccgaca
      721 accccacatg ctcaacatag aaacccacat agcgtaacgg gcgtcacgag cccccttaaa
      781 tccagcgggt agcacccatg aagcggacca aataggcata aataaagcat ttagccaaat
      841 cagaatcaca acgacatgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc
      901 ggcaaaggga gccgttagcc aggcgatggc cgttaatcca agagtggtca gccaaaacac
      961 atgccgcaac tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat
     1021 gatcgtagaa gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgaaaa
     1081 cgcgataaaa tttccggcaa taacactggt ccccattccg gcaacgaaca tttgggttaa
     1141 taaccgacca ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac
     1201 ttcccagata atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc
     1261 aggattaaaa ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat
     1321 ggttaaaccc aacccagccc cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat
     1381 caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca gtagcggtat
     1441 tttcgtattc cctgcaccac gaagtgcccc gctaccaatt agagtgatgg cagctgctgg
     1501 ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt ctgtcgtggc
     1561 atcacccgcg acgaaatcaa taatttgctc gccaaaatga tgaataagcg ttgccaacag
     1621 tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg
     1681 atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga tagcagcaaa
     1741 aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca tcgcatcttt
     1801 tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac aggcattctc
     1861 catgaagata ggaacagcaa acggggttat ctcgcgccag aacaacactt tgtagctctt
     1921 gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg agatattcaa
     1981 agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag aatcagcaaa
     2041 gctgcaaaga ttttcgccat caaattgtct gcaaatgcaa caaactgttg atagaaacgg
     2101 caaacagttg ggaaaattaa aaatcgggtt tgacaaaaga tttttcgccg ttaagatgtg
     2161 cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     2221 cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt tatagcgggc
     2281 tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct gccgcttgat
     2341 atcctcaact gaaagtcctg cattgcaaag ttccagaaag cgccagacat agttacgctg
     2401 aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat gccgcgtatc
     2461 caggcggatt aaattctttt cctcttgttc accgctggat tgttcggcaa ctaatccgat
     2521 cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac ttaatataat
     2581 atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc ccgtaatccc
     2641 ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg gtgtaatttg
     2701 cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc acgggaaggc
     2761 gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag cttcaccatt
     2821 atgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc gaacctccgg
     2881 gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct gagtatgcgt
     2941 cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa gccgacgaac
     3001 attactggct tcattgagaa tacgttctgc aatgaccagt aatgctttgc ccggttcagt
     3061 catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt catcctccag
     3121 ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg caacctctgt
     3181 caggttgtaa tcctgacgtg cagcttcgcg gattatcttt agttgttgga aattcacggt
     3241 aaactccggg cagttcagat atcccgttat tgttaaagtc taacgcccgg cataacaaat
     3301 aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa ttgccactgc
     3361 cttttttcca tcactggaga actaatcact gacattaaca actctttcac tgcctgtgcc
     3421 tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact catggaaggc
     3481 gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc ggccgattcg
     3541 ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtagc aatagactca
     3601 atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa acgcctcatc aacacgaagt
     3661 ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gtgcaatagc attaacatca
     3721 acgctttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt cagcaaagcc
     3781 tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc gagttggtga
     3841 tttatcagtt tttcgttaag cactgcaccg ctattttcat gaagatagat aacgacctcc
     3901 gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct
     3961 ggcgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
     4021 tgggcctgct cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
     4081 tctgttggcg taacgccccg ttttgtgcga atcaaaagtt gttgatttaa ctcaccttcg
     4141 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctga
     4201 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
     4261 cctccggttt ttgagaatcg gcccaagtgc cgccattact tacaaccaga ttgcaagatg
     4321 cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat aagagattat
     4381 gatagattcg tcattggctc ttttcaccag cagtcgcgtt cccctgccct ataaaaggag
     4441 ggtatgcacc acgatggttc attaccaata agattgaaat ctcatcactt tgttgaaatt
     4501 gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca ctgccattaa
     4561 tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     4621 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc ctgctttcta
     4681 gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga accagaacta
     4741 atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt cacatcaact
     4801 tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac ttttcggtca
     4861 gactcatatt cagcacgatt gcgcgacagc ggctcatgaa cttccagcca ctttgaacca
     4921 tccggttccg ttgtgtattt cactggctga tcaataattt gcacacgagt acctacagga
     4981 acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc ctgacttacc
     5041 cggagcccaa taccaaaatt ggcattggta ccgtggatgg catatagcct gccaatataa
     5101 atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg caaactctcc
     5161 cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg tgcttcttgt
     5221 ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg gccgatacca
     5281 ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg catctcagcg
     5341 acgttaacca caatcccttt acgaacagtg acgggcaaaa tcagttgctg cggaatggtg
     5401 agttgcgaac ctgacttcgg taagaagaca tcagcgcccg gattcgcttc cagcatgtta
     5461 cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt gtgatcagga
     5521 acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa tggataagtt
     5581 accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat atttacacga
     5641 cgcatcatgt ccctttccta agtcgcgaaa gctatcggtt aagtatagct tttatcagac
     5701 ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa ttttactggc
     5761 agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg gcatcgccag
     5821 agctgcgcca ctcccctcac ctaaacgcat atccatattg agaaaaggct ccagccccaa
     5881 atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa tgagatacgg
     5941 tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa gaaatccatc
     6001 cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca ttccgaccaa
     6061 atcaaatcca cccacttttg ccagaacatt aacgccatcc tgcggatttg gctgattcaa
     6121 cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg gcaggtttgc
     6181 gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta ttgctgctgc
     6241 cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac cgttttttgc
     6301 cagctcccgc gtataacata tgacgtccaa aagtaacttt tcagcctgac ggcgactcat
     6361 tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga taagcccggg
     6421 gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc ccgcttgtgc
     6481 tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa tggctgtcac
     6541 ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac acataaccag
     6601 taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg ccagttgtac
     6661 gccaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt caatatgccg
     6721 ttgtgcacgc gacatagcgg caggatcgat ggcagggatc gtattcagta aattggcaag
     6781 tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc accaagctct
     6841 atggctgctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag aagctgaccg
     6901 aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat acccggcaat
     6961 aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt
     7021 ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg atacatcaat
     7081 aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag gatcggttcg
     7141 ccacgcagag ccaactcgct taacaccaaa atctttgcca gtacaacaaa aatcaatgcc
     7201 agaccgccgt gggtacctaa acggctatca cgcatgattt ccaacatgcg atcgcggcta
     7261 cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa tcccccggtc
     7321 atcagcacca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc
     7381 agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa aggaaaagta
     7441 ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg cggtacaggc
     7501 agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt tttactccaa
     7561 tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga tttacccgcc
     7621 cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc attcccactt
     7681 cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc aacgactgaa
     7741 tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg tctttatcgc
     7801 cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc aacacaacct
     7861 cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc tccactgtgc
     7921 gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc tcatcatcaa
     7981 ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     8041 ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg actcctgatt
     8101 caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     8161 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt ctcaatccat
     8221 tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt ccgacaaaat
     8281 gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg aattaatgct
     8341 actgccggaa caa
//
LOCUS       DAZGXS010000173         8101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGXS010000173 DAZGXS010000000
VERSION     DAZGXS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            113..589
                     /locus_tag="ACHKG5_004272"
     CDS             113..589
                     /locus_tag="ACHKG5_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0870253.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            586..2709
                     /locus_tag="ACHKG5_004273"
     CDS             586..2709
                     /locus_tag="ACHKG5_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0870254.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGREAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2706..2918
                     /locus_tag="ACHKG5_004274"
     CDS             2706..2918
                     /locus_tag="ACHKG5_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870255.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2918..4420
                     /locus_tag="ACHKG5_004275"
     CDS             2918..4420
                     /locus_tag="ACHKG5_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870256.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4365..6389
                     /locus_tag="ACHKG5_004276"
     CDS             4365..6389
                     /locus_tag="ACHKG5_004276"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0870257.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6477..6803
                     /locus_tag="ACHKG5_004277"
     CDS             6477..6803
                     /locus_tag="ACHKG5_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP0870258.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6796..7077
                     /locus_tag="ACHKG5_004278"
     CDS             6796..7077
                     /locus_tag="ACHKG5_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0870259.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7080..7703
                     /locus_tag="ACHKG5_004279"
     CDS             7080..7703
                     /locus_tag="ACHKG5_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870260.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARGLAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            7716..>8101
                     /gene="gpU"
                     /locus_tag="ACHKG5_004280"
     CDS             7716..>8101
                     /gene="gpU"
                     /locus_tag="ACHKG5_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP0870261.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
BASE COUNT         1876 a         1928 c         2540 g         1757 t
ORIGIN      
        1 attaaaaaga tgatgattgt ggttgttttt tgttcgacat ctttatatgg cggcatttct
       61 ttacaaaaaa acagagtcac ttctgttctg cggtttatgt ggagggatgt aaatggacgg
      121 cgagctgaaa aatatgaagt taaatattaa tcaactggca gccctttcag gtctgcaccg
      181 gcagactgtt gccgccagaa tggcggatgt tcctcttgca ccaggcagta atgaaaagaa
      241 aaaactgtat ctcctgacgg atttgataac ttcgttgctg gaaaaaccac catcttccga
      301 agatgaggat atggatcctc acgctcgtaa ggcatggtat cagtccgagc gcgagcgtct
      361 taaatttcag catgaaactg ttcagcttgt gccagtcagt gatgtcaggc ggtccttttc
      421 tgtcgtggtg aaagcgatag ttcaggtact ggaaacctgg cctgaccggc tggagaggga
      481 cagggggtgg actgcatcac aactgaatga agtacagatt gtggttgatg agatccgcga
      541 cacactggaa aaggcagtca ttgactgttg tgatgaggcc gatatgtgaa tcaggtgaac
      601 gagagccata gccgcgcatc cgatatctgg cgcgaagtgg cctcgctgtt tcgcccaccc
      661 agccggttac cagtagcgga agccatcagg cgttatatgc gggttccacg gggagccaat
      721 acttccggtc cgtgggagtc atcgctgacg ccctatatga ttgaccccat taatacatta
      781 tcagcccgtg aatatgacgc ggtggtgttt gtgggacctg cgcgaaccgg gaaaaccgaa
      841 gggctgattg atggctggat tgtgtacggc atcatctgtg atccggcgga tatgctggtg
      901 gtgcagatga ctgagacgaa ggcgcgtgag cattccagaa cgcgtctttc caggacgttt
      961 cgccacagtc cggaggtcag caagcgcctc agtccttccc gtaatgacaa caacgtccac
     1021 gataaaatgt ttcttgacgg ctccttcctg aagattggct ggccgtcgat caccgtcttt
     1081 tcctcttcgg attaccgtcg tgtggcgctg acggattatg accgtttccc tgaaaatgtg
     1141 gacggggaag gggatgcctt cacgctggcc tcaaagcgta ccaccacctt tatgtcctcg
     1201 gggatgaccc tggtcgagag ttcaccgggg cgggatatca ccgataccaa atggcgttgt
     1261 ggtggcgcac atgaggcacc gccaacaacg gggatcctgt cactgtataa ccggggagac
     1321 cgccgccggt ggtactggcc gtgtccgcac tgcggggaat attttcagcc ggtgatggat
     1381 aacatgaccg gataccggaa taacccggat tttgtggctg ccggtcaggc tgcccgtctg
     1441 atgtgtccgc attgtcgcgg gctgattgcc cctgagcaga aacgcgaact gaataaccag
     1501 gggatctggc ttcgtgaagg tgaacgggcg gcggcggacg gcagtatcac cggaacgcca
     1561 cgaaactccc ggattgcgtc attttggatg gaggggccag ctgcggcgtt tcagacctgg
     1621 gaacaactga tttttaaact gctggcggca gaagaagagt atgagcgaac cggcagtgaa
     1681 gagaccctga aagcggtggt gaacaccgat atcggacgac cctatctgcc ccgttcagcc
     1741 acggaacagc gtaaaagtga actgcttgaa cagcgtgccg agccgtttcc ccggcgatct
     1801 gtgccggatg gtgtgcgttt tattgaggca acggttgacg tacagggcgg taaaaatcgc
     1861 cgttttgttg tgcagatcac cggatacgga gagcaggggg aacgctggat tgttgatcgc
     1921 tacaacatcc ggcattcact gcgctgcagt cccaacggtg aaagtctgcc ggttgatccg
     1981 gcggcatatc cggaggactg ggatttgttg ctgacggatg tgttccataa aacatggccg
     2041 ctggcttctg atccggatgt gcgcatgcgt ctgatggcca tggcggtgga tacgggaagg
     2101 gaagccgggg tgacagataa cgcctatcgt ttctggcgtc gttgccggag tgacggactg
     2161 ggcaacaggg tgtttctgtt caagggggat ggacttcgcc gtgacaggct gattaaccga
     2221 accttcccgg ataataccgg cagaagtgcc cgccgtgcca gagccagtgg cgatgtcgcg
     2281 ctgtggctgg ttcagacgga tgcgtttaag gatcgtgtaa ataatgccct gtggcgtgac
     2341 acaccagggc cgaactatat ccactttccc gactggctgg ggcggtggtt ttacgatgag
     2401 ctgacctatg aagagcgcgg cagtgacgga aaatggcgaa aaccgggcag gggcgctaac
     2461 gaagcgtttg acctgctggt ttatgcggat gcgcttgccg ttctgcatgg ttacgaaaag
     2521 atccgctggc cctccgcacc ggactgggca cagcgggaaa cgtggctcgt cttcccgcag
     2581 gagcgttctg gtgaaacggt atccccggaa ctgacggccg gggcagaaaa acgccgtcgc
     2641 cggaagaaaa aactgcggac ggagcgtgcg gaagataatc catggataac atcaggaggc
     2701 tggttgtgag cacagaagaa gccagagaaa tgatacagcg gtaccgtgaa gcggaaatgg
     2761 ccgtactgga gggaaagtct gtcaccttca acgggcagca actgacgctg gaaagccttt
     2821 ctcagatccg cgccggacgt caggagtggg aacgcaggct tgccgcgatg gtgagccgca
     2881 ggcggggaaa accgggattt aaactggcga ggttttaatg gcaattattg atgatgtgat
     2941 cggcgtgttt tcccccgggt ggaaagcagc cagactgcgt tcaagggcgt taatcatggc
     3001 ctatgaggcg gtgaaaccga cccggacaca taaagcccgg cgggaaaatc gctctgctga
     3061 tcagctcagt aaatacggtg cggtttccct gcgggagcag gcccgttttc tggatatcaa
     3121 tcatgacctg gtgattggtg tgtttgacaa gctggaagag cgggtgattg gtgccagggg
     3181 aattattgtg gagcctcagc cattacgaaa aaacggggaa atggcggctg agctggctgc
     3241 ggatatccgc cgtttgtggg ctgaatggtc cgtgagtccg gatgtgacag ggcagtatac
     3301 ccgtcctgtg cttgaacgtt tactgctgcg gacctggctg cgggatggtg aagtgtttgc
     3361 gcagatggtc agtggtgcgg gaaacggtct ggaacggaca gcgggagtgc cattctggct
     3421 tgaggcgatg gagccggatt ttgttcccat gcgcactgat gaatccgccg gactgaatca
     3481 gggggttttt cttgatgagt ggggaagacc gaaaaaatat ctggtttata aaaattatcc
     3541 ggtcagcggc cggcagagtg atacgaaaga aatcgctgcc ggaaaaatga tccacctgaa
     3601 gttcactcgt cgtctgcatc agacgcgagg ctcatccatg ttatcggggg tgctgatgcg
     3661 gatcagtgcc cttaaggagt atgaggatgc ggaactgaca gcggcgcgta ttgctgcggc
     3721 gctgggactg tatatccgta aaggtgacgg acaggactat gaagatccgg ggatcaaaga
     3781 gaccgagcgg gaagtccata tcaccccggg tattatttat gacgatttgc gcaagggcga
     3841 ggatatcggc atggtcaaat ctgaccgtcc caatcccaac cttgaaactt tccgcaacgg
     3901 ccagttgcgt gcagtggcag caggcagtcg tctgagtttt tccagtgcgg cgcgtaacta
     3961 taacggcacc tacagcgccc agcggcagga gctggtcgag tccacggatg gttacctgat
     4021 cctgcaggac tgttttattg gcgcggtaac ccgcccggtg taccggacat ggctgaatat
     4081 ggtggttgcg gcaggtctgc tgaaaattcc ggcggatgtg gagatgaaaa cgctatataa
     4141 cgcgacgtat tccggtccgg tgatgccgtg gatcgacccg gttaaggaag ctgaagcctg
     4201 gagaattcag atccggggtg gtgcagcgac agaatctgac tgggtgcgtg ccggtgggcg
     4261 caatccggat gaggtcaaac gtcgccgcaa ggctgaaatt gatgaaaaca gcagactggg
     4321 gctggtcttt gatactgacc ccgtcaacga caaaggaggc aacagtgccg gaactgaacg
     4381 acagtatcag cgcgacaccg aaagccagca tgaagaataa atcctggttc aggatgcaag
     4441 ctggggggcc gggtgacgcg gatatttata tttatgacga gattggtttc tggggagtta
     4501 ccgcgaagca gtttgtcagc gaactgaatg cactgggtga tatcacccac attaatctcc
     4561 atatcaattc accgggtggc gatgtctttg aaggcatcgc catttttaat gccctgaaaa
     4621 atcagggggc gaccattacc gtgtatgtgg atggcgttgc cgcctcgatg gcatctgtga
     4681 ttgcgatggc cggtgatacg gtcattatgc cggaaaatgc cttcatgatg atccataagc
     4741 catggggatt cagtggcggg gatgctgagg atatgcgcag ttatgccgat ttgctggata
     4801 aagtcgaatc ggtactgttg ccagcctatg cgcagaaaac cggaaaaacc accgatgaaa
     4861 ttgccgccat gctggcggat gaaacctgga tgtccggtgc cgaatgtctg gcacacggat
     4921 ttgctgacca ggtgacaccc gctgttgagg caatggcatg tattcagtca aaacgtacag
     4981 aggaatttaa aaagatgccg gaatccatcc gaaacatgat tactccgcca cgcaacagtg
     5041 ccccgcgtga taccacagtg acaatccctg caccggcggt aacagaacca tcaccggtac
     5101 cggcagtgtc tgatgaggcg accattcgcg cccgcgttat ggctgagcag aaagcccgca
     5161 tgtcaggcat taacgatctg tttgccatgt tcggcggtcg ctatcagacg cttcaggcac
     5221 agtgcgtggc tgatcctgac tgttcgctgg aaatggcccg tgaacgtctg ctgaatgaaa
     5281 tgggcaagga gtcctcgccg accaacaaaa atacaccggc ccatatttat gccggaaacg
     5341 gcaattttgt gggggacggg atccgccagg cgatgctggc ccgtgccgga tttgaaaatg
     5401 tcgagaagga taacgcctat aacgggatga ccctgcgtga atgggctcgc atgtcactga
     5461 cggagcgcgg tattggggtg gccagttata accccatgca gatggtcggg ctggcgctga
     5521 cgcacagcac ctctgatttt ggcaatattc tgctggatgt gtcgaacaag gggctgatcc
     5581 agggctggga ggaatcagaa gaaaccttcc agaagtggac ccgtaaggga cgcctgtcag
     5641 acttcaaaac agcgtatcgc gtggggatgg gcggttttgg ttctctgcgc caggttcgtg
     5701 agggggcgga gtataaatac atcaccacct cagatcgcaa ggagaccatt gcactggcca
     5761 cttacgggga gattttctcc atcacccgcc aggccattat caatgatgat ctgaatatgc
     5821 tggtggacgt gccgatgaag atggggcgtg cggcgaaggc aacgattggt gacctggtct
     5881 acaaggtgct gacggataac ccgaaactgt ccgacggtaa ggcgctgttc catgccgatc
     5941 acaaaaatat tgccaccggg gggatctccg tttccggact ggatgcggcc cgtcagatga
     6001 tgcgcctgca gaaagaaggc gatcgtgccc tgaatatccg tccggccttt atgctggtac
     6061 cggtggcact ggagacggtg gcgaaccaga ccatcaaatc ggccagtgtg aaaggggcgg
     6121 atgcaaacgc cggtgtcatt aaccctatcc agaactttgc tgaggtgatt gcagaagcgc
     6181 gtcttgatgc ggcagacccg aaaacctggt atctggcggc ggcacagggc actgacacca
     6241 ttgaagtggc ctggctggat ggtgtggaca cgccatacat tgatcagcag gaaggtttca
     6301 ccactgacgg cattgccaca aaaatccgta ttgatgccgg agtggcacca cttgactggc
     6361 gcgggctggt gcgttcgtcg gtggcctgat aaccgcgtta tcacaatcac tgcccgaaag
     6421 ggcttttttt atgcctgaaa aacagcccca caggggctgt ccggagaaac agcattatgg
     6481 cgaaaaattt tgtacaggac ggtaccacca ttgaactggt gaatgccgga gatcagacca
     6541 tcctgagcgg tgctgcggtg gtggtcggca gtatggtggc cgtggccatt accgatattc
     6601 ctgccggtga ggccggtgac ggttttgccg aaggcgtgtt cctgctgccc aaacagtctg
     6661 ctgacgacat tcagtccggc gcggtggttt atctgaagga cggggttgtg cagttggctg
     6721 ccgacggtgc ggtggccgcg ggggtagcct gggaaaatgc tcctgcaaac agcgccactg
     6781 tggcggtaaa aatcaatgtc tgatctgttt acgcgaatgt gttgccggat ggacgtggcg
     6841 accgttcgga tgatgggcaa acaggcggag attaacggcg tcgtgtacga cgtgatgccg
     6901 gaggaagagt ccgcggagat gggggcgctt tcgggcagcc agttgtcact ggtggtgttt
     6961 tcagcccggt accgtccggc ccgtcatgat gttgttgtgt ttgcggggcg cacactgacg
     7021 gtgacccgtt atgacacgta caacggtaaa ccccggattt ttgtcgaaca ggaatgagta
     7081 tggcaataaa aggtctggcg caggccatga aaaatctgga tgcaattgac cgccgtgccg
     7141 ttccccgggc tgccgccacg acacttaacc gtgtggcgga gtccatcatc gcgaaaacgg
     7201 cctcttcggt tgccaggggg ctggcggttc cgcgccgtct catccgtgag cgtatccgcc
     7261 tgcaacgggc cagcgcagac agggtttatg cgaaggtcat catcaacaca ggtaatctgc
     7321 ccgccataaa actggggacg gccagcgtgc ggctttcccg cagaaagcga cgaaagaaag
     7381 gcgagcgttc ggtcacgaaa ggcggtggca gtgtgctgat tgtggggaaa agacggatcc
     7441 cggatgcctt tatcacccgg ctggctaacg gacgctggca tgtgatgcag cgtatgccgt
     7501 gggcatcatc gtccaccggc gctgacagca aagggaggcc gaaacgccac cgtctgccga
     7561 tcgaagtggt gaagattccg actgccggac cgctggcaga aacctttgaa cgtgaacggg
     7621 accggatgta ccgggaaaaa ttaccggcgc agatgatgaa agccatgacg catcagttac
     7681 gcctggtgct gaaaagaaaa tgactgggag ggtgtatgaa acaccgtgaa atacgggcgg
     7741 cagttctgtc tgccctgaaa gaaaatattt ctgagagggt gagctggttt gacggtcgcc
     7801 cggtttttat tgatgaacag gaactgcctg ctgttgctgt ttacctgaca gatgcgtctg
     7861 ctgctgacga gttcgttgat gagggaacct gggaggcgac actgcatatt gaggtttttc
     7921 tcagggcaaa agaaccggac tcggcactgg atatgtggat ggaagaaaaa attcttcctg
     7981 cgctggaggc agttcccggg ctcagtgcat tactgctgaa gatgaatctt caggggtatg
     8041 actaccgccg ggatgatgag tttatgatgt ggggatcggc agatctcctg tggaaaatta
     8101 c
//
LOCUS       DAZGXS010000174         8038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGXS010000174 DAZGXS010000000
VERSION     DAZGXS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(68..259)
                     /locus_tag="ACHKG5_004281"
     CDS             complement(68..259)
                     /locus_tag="ACHKG5_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0870262.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKG5_004282"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKG5_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0870263.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ACHKG5_004283"
     CDS             1018..1203
                     /locus_tag="ACHKG5_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870264.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ACHKG5_004284"
                     /pseudo
     CDS             complement(1390..1779)
                     /locus_tag="ACHKG5_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHKG5_004285"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHKG5_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP0870265.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /locus_tag="ACHKG5_004286"
     CDS             complement(1921..2076)
                     /locus_tag="ACHKG5_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0870266.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ACHKG5_004287"
     CDS             complement(2354..2641)
                     /locus_tag="ACHKG5_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0870267.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ACHKG5_004288"
     CDS             complement(2641..2832)
                     /locus_tag="ACHKG5_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP0870268.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ACHKG5_004289"
     CDS             complement(2860..3261)
                     /locus_tag="ACHKG5_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870269.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ACHKG5_004290"
     CDS             3370..3642
                     /locus_tag="ACHKG5_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0870270.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ACHKG5_004291"
     CDS             3626..4051
                     /locus_tag="ACHKG5_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0870271.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ACHKG5_004292"
     CDS             complement(4258..4713)
                     /locus_tag="ACHKG5_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870272.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5883
                     /locus_tag="ACHKG5_004293"
     CDS             4792..5883
                     /locus_tag="ACHKG5_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870273.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5890..6636
                     /locus_tag="ACHKG5_004294"
     CDS             5890..6636
                     /locus_tag="ACHKG5_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0870274.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6658..7428
                     /locus_tag="ACHKG5_004295"
     CDS             6658..7428
                     /locus_tag="ACHKG5_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0870275.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7444..7857
                     /locus_tag="ACHKG5_004296"
     CDS             7444..7857
                     /locus_tag="ACHKG5_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0870276.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2145 a         1726 c         2096 g         2071 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataacttacc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatcg ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gttgattgca tacagttttt tattgtaggc tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggagcagg agaaagaaca ggaacaggac aaaaacacta tggttcatgg caaaaaaaac
     5341 accacgaacc aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca
     5401 ccggaagccg attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga gcaatcacac
     5461 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
     5521 gaaagcaccg ggcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     5581 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
     5641 gccagattgc gcgagggggt gccaccagag accatgctgg atggtgtgag gcgttacgcg
     5701 agatacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgagc gacgacgttt
     5761 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
     5821 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
     5881 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
     5941 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgct
     6001 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
     6061 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     6121 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     6181 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     6241 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     6301 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     6361 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     6421 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     6481 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gtacacttct tggtgagagg
     6541 attatggacc gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg
     6601 cgtccaaatg tcagcaatat gagggttgtg aagtaatttt gtccggagga aattttaatg
     6661 gaaaccgtat ctgacgcact gaaagcactg aaaaaagcct cttcacatgt ggtggcagct
     6721 cgccttggaa tcagtcgtga agaggctgtc aacgagctgt gggaactcaa aataaatggc
     6781 gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc
     6841 gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa
     6901 gttaccgcag acatgatgat tgagtttatc ggtcaggatg gggctaaaac gtgtgaggaa
     6961 ctggcgggta agttcggtgt cagtactcgc aaggttgctt ccacgctggc ggtggtaacc
     7021 gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc
     7081 gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt
     7141 cctcagccag caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa
     7201 acagaaactg tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat
     7261 gagctgattt ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat
     7321 gttcagaagt gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt
     7381 gttcgacaga ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg
     7441 cttatggcaa aagtatttac acaggaagag cgggaaaaaa ttaaagggca ggttgttgag
     7501 ctagtacgcc ggagtgggcg cgagacgtta cggcaactgg aagccaagac aggtgcgaca
     7561 agatatctga tgagcgttct tgccagagag ctggttgcca gtggcgatgt atacaactct
     7621 ggttacgggt tattcccatc tgaacaggct cgtaaggact ggcaaaacgc ccgcaaaaaa
     7681 ctatcgaggg caaaactgaa gaaaccggtt gtggttgatc ctgaccttat ctggtcatta
     7741 ccagacggag aaatacgccg ctatgacagg caattgaaca taatttgtag tgagtgccgt
     7801 aacagtgaag tgatgcagcg agttttgata ttttacacag gagtaatgat ggaatagtga
     7861 aaataacatg aaacctttgg attctggtgt ttcagtggat gggaaaagag cagatacata
     7921 gaaaatgaat agcaataatt cacagtctgg atgttgtttg tatgcttata aaatgatcct
     7981 agtatgcatt gccggagttg aatcagctcc aatgaagaaa ctgagaggag tatttaga
//
LOCUS       DAZGXS010000175         7975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGXS010000175 DAZGXS010000000
VERSION     DAZGXS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            2..205
                     /locus_tag="ACHKG5_004297"
     CDS             2..205
                     /locus_tag="ACHKG5_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YafQ family addiction module toxin"
                     /protein_id="HFP0870277.1"
                     /translation="MNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWI
                     LIYKLTDKLLRFERTGTHAALFG"
     gene            361..1101
                     /gene="dpaA"
                     /locus_tag="ACHKG5_004298"
     CDS             361..1101
                     /gene="dpaA"
                     /locus_tag="ACHKG5_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0870278.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1072..1839)
                     /locus_tag="ACHKG5_004299"
     CDS             complement(1072..1839)
                     /locus_tag="ACHKG5_004299"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0870279.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1944..2522)
                     /gene="lpcA"
                     /locus_tag="ACHKG5_004300"
     CDS             complement(1944..2522)
                     /gene="lpcA"
                     /locus_tag="ACHKG5_004300"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0870280.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2762..5206
                     /gene="fadE"
                     /locus_tag="ACHKG5_004301"
     CDS             2762..5206
                     /gene="fadE"
                     /locus_tag="ACHKG5_004301"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0870281.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5249..5695)
                     /gene="ivy"
                     /locus_tag="ACHKG5_004302"
     CDS             complement(5249..5695)
                     /gene="ivy"
                     /locus_tag="ACHKG5_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0870282.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5876..6646
                     /gene="yafV"
                     /locus_tag="ACHKG5_004303"
     CDS             5876..6646
                     /gene="yafV"
                     /locus_tag="ACHKG5_004303"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0870283.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(6764..7936)
                     /locus_tag="ACHKG5_004304"
     CDS             complement(6764..7936)
                     /locus_tag="ACHKG5_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFP0870284.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1913 a         2014 c         1972 g         2076 t
ORIGIN      
        1 tatgaataaa ttgaaatatc ttatgacgct tcttatcaat aatactttac cgcttccagc
       61 tgtttataaa gaccacccgc tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga
      121 accggactgg atcctgattt acaaacttac cgataaactt ttacgatttg agagaactgg
      181 aactcacgcg gcgctctttg ggtaaagccc gctgccgatg aaatttgtaa accctgcgcc
      241 gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttcccaatcc agactattcc
      301 gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt
      361 atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt
      421 ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca gcagttgatg
      481 ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa
      541 atgggcgagc aatatcaact gctcgacagc tataaaatct gtaaatattc cggcggctta
      601 gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag cgtccagcgt
      661 aatcagttaa aaccagacag ccgttactac aaagcgatta atattggttt ccccaatgcc
      721 tatgaccgtg cgcatggtta tgaagggaaa tacctgatga tccacggcga ttgtgtttcc
      781 atcggctgct acgcgatgac caatcagggc attgacgaga ttttccagtt cgttactggc
      841 gcactggtat ttggtcagcc gagcgtgcaa gtgagcattt acccgttccg catgaccgac
      901 gccaatatga agcgccataa atattccaac tttaaggact tctgggagca actgaagcca
      961 ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt caatggccgt
     1021 tacgtggtca gcaagccgtt aagccacgaa gttgtgcaac cacaactggc atcaaactac
     1081 acgctccccg aggcaaaata agcgccattc gcctggcata atcttttgcc aggtttcatt
     1141 gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg tctgcgagct
     1201 gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg cgcggcgggt
     1261 gatccagtgc agattggtcg agcaatacgc cattacatag cgcccatccg aaagcagcat
     1321 gttgaaaacg cccttctgcc gcagttcatc cgccagtgag gcgatatatt taaataccgc
     1381 cgccatattg cccggcgtac gcggataacg ctgcgttaat ttatacagca gccagcaaaa
     1441 ggctttttcg ctgtcggttt tacctaccgg gcggaagttg ccggtttcca gtgatttata
     1501 gcccgtcagt tgtccgttat gggcataagt ccagttacgt ccccataact cgcgggtaaa
     1561 cgggtgagta ttttccaacg ccacctcgcc ccgatttgcc tggcgaatat gagccacaac
     1621 cgaacaggat tttatcgggt agtcctgaac aagtttggca atgggggaat taaagctggg
     1681 ttgtggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc cccagccatc
     1741 tttatgtggc ccggttccac caccacgctg caccagcccg gtgaaactaa agcaaatatc
     1801 ggtagggacg ttggcgctca tcccgagcaa ttcgcacatc ggtaaccccc acaacagcgg
     1861 gagcgcatgg cccccggcga agattgactt aagaattttc aggccggatg cggcgtaaac
     1921 gcattattcg gcctacgcca gacttactta accatctctt tttcaattaa ctggatcagg
     1981 atatggatca ctttaatgtg aatttcctga atgcggtcgg cataaccaaa atgtggaacg
     2041 cgaatttcaa tatccgccgt accagccatt ttgccgccat ctttgccggt cagggtaatc
     2101 actttcattc ccttctcacg cgccgccgcg atcgctttga tcacgtttgc agagttacca
     2161 gacgtggaga tccccagcag tacatcgcct tcgcggccta ccgcttcaac atagcgggag
     2221 aaaatatcat tgaaaccaaa atcgttaccg acgcaggaaa tatggctgac gtcagagatg
     2281 gcaatcgccg ggtagcccgg acggttttca cggtagcgac cggtcagctc ttcggcaaag
     2341 tgcatggcgt cgcaatggga gccgccgttg ccgcaggaaa gcaccttgcc accggcttta
     2401 aagctgtctg ctaacaggac cgccgctcgc tgaatggcgt gaatattggc gtcatctttt
     2461 aaaaagttag ccagcgtttc cgccgcttcg ttcagttcgt tacgaataag atcctggtac
     2521 atgaggatat ccttcagcat aaatgtaata gacaaaatgc agtgtaccgg ataccgccaa
     2581 aagcgagaag tacgggcagg tgctatgacc aggacttttt gccctgaagt gtagataaaa
     2641 acagcaacaa tgtgagcttc gttgtaatta tattgtaaac acattgctaa atgtttttac
     2701 atccactaca accatatcat cacaagtggt cagacctcct acaagtaagg ggcttttcgt
     2761 tatgatgatt ttgagtattc tcgctacagt tgtcctgctc ggcgcgttgt tctatcaccg
     2821 cgtgagctta tttatcagca gtctgatttt gctcgcctgg acagctgccc tcggcgtcgc
     2881 tggtctgtgg tcggcgtggg tactggtgcc tctggccatt atcctcgtgc cgtttaactt
     2941 tgcacctatg cgtaagtcga tgatttccgc gccggtattt cgcggtttcc gtaaggtgat
     3001 gccgccgatg tcgcgcactg agaaagaagc gattgatgcg ggtaccacct ggtgggaagg
     3061 cgacttgttc cagggcaagc cggactggaa aaagctgcat aactatccgc agccgcgcct
     3121 gaccgcagaa gagcaagcgt ttctcgacgg cccggtagaa gaagcctgcc ggatggcgaa
     3181 tgatttccag atcacccatg aactggcgga tctgccgccg gagttgtggg cgtatctgaa
     3241 agagcatcgc ttcttcgcga tgatcatcaa aaaagagtac ggcggcctgg agttttcagc
     3301 ttatgcccag tctcgtgtgc tgcaaaaact ctccggcgtc agcgggatcc tggcgattac
     3361 cgtcggcgtg ccgaactcat taggccccgg cgagctgctg caacattacg gcactgacga
     3421 gcagaaagat cactatctgc cgcgtctggc gcgtggtcag gagatccctt gctttgcact
     3481 gaccagcccg gaagcgggtt ccgatgcggg cgcgattccg gacaccggga ttgtctgcat
     3541 gggcgaatgg cagggccagc aggtgctggg gatgcgtctg acctggaaca aacgctacat
     3601 tacgctggca ccgattgcga ctgtgcttgg gctggcgttt aaactctccg acccggagaa
     3661 attactcggc ggtgcagaag atttaggtat tacctgtgcg ctgatcccga ccaccacgcc
     3721 gggcgtggaa attggtcgtc gccacttccc gctgaatgtg ccgttccaga acggaccgac
     3781 gctcggtaaa gatgtcttcg taccgatcga ctacattatc ggcgggccga aaatggccgg
     3841 gcaaggctgg cggatgctgg tggagtgcct ctcggtaggc cgcggcatca ccctgccttc
     3901 caactcaacc ggcggcgtga aatcggtagc gctggcaacg ggcgcgtatg ctcacattcg
     3961 ccgtcagttc aaaatctcta tcggtaagat ggaagggatt gaagagccgc tggcgcgtat
     4021 tgccgggaac gcttatgtaa tggacgctgc ggcatcgctg attacctacg gcattatgct
     4081 cggcgaaaaa cctgccgtgc tgtcggctat cgttaagtat cactgtaccc accgcgggca
     4141 gcagtcgatt attgatgcga tggatattac cggcggtaaa ggcattatgc tcgggcaaag
     4201 caacttcctg gcgcgtgctt accagggcgc accgattgcc atcaccgttg aaggggctaa
     4261 cattctgacc cgcagcatga tgatcttcgg acaaggagcg attcgttgcc atccgtacgt
     4321 gctggaagag atggaagcgg cgaagaacaa tgacgtcaac gtgttcgata aactgttgtt
     4381 caaacatatc ggtcacgtcg gtagcaacaa agttcgcagc ttctggctgg gcctgacgcg
     4441 cggtttaacc agcagcacgc caaccggcga tgccactaaa cgctactatc agcacctgaa
     4501 ccgcctgagc gccaacctcg ccctgctttc tgatgtctcg atggcagtgc tgggcggcag
     4561 cctgaaacgt cgtgagcgta tctcggcacg tctgggtgat attttaagcc agctttacct
     4621 cgcctctgcc gttctgaagc gttatgacga cgaaggccgt aatgaagccg atctgccgct
     4681 ggtgcactgg ggcgtacagg atgcgctgta tcaggcagaa caggcgatgg acgatttact
     4741 gcaaaacttc ccgaaccgcg tggttgccgg gctgctgaat gtggtgatct tcccgaccgg
     4801 acgtcattat ctggcaccgt ctgacaagct ggatcataaa gtggcgaaga ttttacaagt
     4861 gccgaacgcc actcgctcac gtattggtcg cggtcagtac ctgacgccaa gcgagcataa
     4921 cccggttggt ttactggaag aggcgttggt ggatgtgatt gccgccgacc cgattcatca
     4981 gcggatctgt aaagagctcg gtaaaaatct gccatttacc cgtctggatg aactggcgca
     5041 caacgcgctg gcgaaggggc tgattgataa agatgaagcc gctattctgg tgaaagcgga
     5101 agaaagccgt ctgcgcagta ttaacgttga tgactttgat ccggaagagc tggcgacgaa
     5161 gccggtaaag ttgccggaga aagtgcggaa agttgaagcc gcgtaattga ataacggagc
     5221 cgaaaggctc cgtttcttta tccgctaatt atttaaaatt aaagccatcc ggatggtttt
     5281 ccaggctgcc ggtcaacgcc gcgaacagca ccgttttacc atcaatcgaa agcgcatcgt
     5341 tcacattcaa ccaggtcagt ttctcttgcg acgttttctc atcaatagtc gagaacagcc
     5401 ccgtcatctg attagatttc tcggaccaca tcacagcgat acgttgcgag ccacagtcat
     5461 gcggtttgca cgcgctcatc acctgatacg tctcatctcc caacgttacg gtttgtgcag
     5521 gggtataagt accgcctttc atcacccagg caggcagctt atgcccttgc accatctgat
     5581 taaatgcagc tttggtggtt tcgccctttg caaggctgct aatggttaaa tcatcctgcg
     5641 ccattgcact ggtggcgatg accagtgcgg cgactgtcgt tattgcctta aacatcattc
     5701 ctcccgagct tatcctgccc atgttattaa cctcctaaag cctggcgata acacgataat
     5761 ttgcaaatca gatatttcca atatcagcat ttgttatcaa tttattctgc gctaaggcaa
     5821 ctgcctgata aggtgttaaa gttataaaac ccttcgcata aaggagttgt acaccgtgcc
     5881 tggtttgaag attacgcttt tgcagcaacc actggtgtgg atggatggtc ctgccaacct
     5941 gcgtcatttt gatcgtcaac tggaaggtat taccgggcgc gatgtgatcg ttctaccgga
     6001 gatgtttacc agcggctttg ccatggaagc ggctgcttcg tcgctagcac aagatgacgt
     6061 agtgaactgg atgacagcca aagcgcagca gtgcaatgcg ctgattgcag gcagtgttgc
     6121 attacaaacg gagtctggtt cggttaaccg ctttttgctg gttgagccgg gcggcacggt
     6181 acatttttat gataagcgtc atctgttccg catggcagat gagcatctgc attataaagc
     6241 gggcaatgcg cgagtgattg tggaatggcg cggctggcgt attttgccgt tggtgtgcta
     6301 cgacttacgt tttccggtgt ggtcgcgcaa tctcaacgat tatgacctcg ccatctacgt
     6361 tgccaactgg cctgctccgc gctctctgca ctggcaggca ttgctgacgg cccgcgcgat
     6421 tgagaatcag gcgtatgtgg cgggatgcaa tcgcgtcggc agcgatggca acggctgcca
     6481 ttatcgcggt gacagccggg tgattaatcc acaaggcgag attatcgcta ctgccgacgc
     6541 gcatcaggca acgcgcattg atgcggagct gtcgatggtg gcattgcggg aatatagaga
     6601 aaagtttccg gcatggcggg atgcggatga gtttaggttg cggtgagtgg gggctgtatt
     6661 tagggcgggg aattcagaaa gggatgttta gtgtgctgag cgagagttaa ataataaaaa
     6721 actgggaaga tttaataatc cccccgcagg gcaagagcat gaattaaatc tgtgttaaat
     6781 ttttcatttt gtcagttcga aacccaagct ctgagcagga tgcaagcact tcttctatat
     6841 aacatgtgtt taacgcaacc ttctttcttt tcctcttcac cccaccctta atgtctttgc
     6901 aatctcttaa taaattcagt gccatcttct ttattccaga tattatttcg gctgcgtttc
     6961 ctcttcttat ccggctggca tcttcattca tttttacatc taacacccaa tgaagactgt
     7021 gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc tttagcatcc atatcctttg
     7081 atgaaatata atatcggatg cttacacctt ctgctgattt atcttctttc ttctgcctga
     7141 atgacaatgc tacacaaagc ttttttaatc ccttccattc gaattcaaaa tcacaaaatt
     7201 caggcgttac gttactgaca atatgcaaac gtgtttcttt tcttccatga cttatctcct
     7261 gcgtactaaa cgaatcgcct ttataattag aaaacacatt tacaggaaat ttttcctcga
     7321 atgcatgatg taatttcccc tgattgcctt ttactgccag aagataatct gcttttttat
     7381 ctttgatctt cgaagcgata tctttctgac agcccatagc atcaatggtt atcaaatttt
     7441 tctttaaatc cagtaggtta agcaactctg gaatggctgt aatctcatta cttttggctt
     7501 ccgtttttac ctgccccagt acaacaccat tttcgttcga gaatgcactc accatatgga
     7561 ttgctccttt tctttttccc ttatcaaagg agcctcttat ggtctttcca tctattgcta
     7621 taatttcacc atcagtgatt tcatggcact cctgcatcca ttcaataaat atcttttcaa
     7681 aggccaaact gtcaatgtta ctcacaacgc gtgcaatggt gtcatcgacc ggaatgccat
     7741 tatcaaaatc accatatttc tttagccatt caagtctttc atgtccaaaa tcttcaattt
     7801 cctgccactc atcggcacct gcaattactg cacatacggt gagaaacaaa atatcagata
     7861 atttatgttt aacctttcct tgttgtcgta tatcagggat cactgaaata taatcaagca
     7921 aactttgaat actcacctgg cgtccttttt atctttttag ttattccaaa ggtta
//
LOCUS       DAZGXS010000176         7914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGXS010000176 DAZGXS010000000
VERSION     DAZGXS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..854
                     /locus_tag="ACHKG5_004305"
     CDS             <1..854
                     /locus_tag="ACHKG5_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP0870285.1"
                     /translation="STSSLLIWFPCLFLVCGWLYLWKRRPQFTSVDVLRMQKFYLPRK
                     KSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLEL
                     LFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGY
                     LTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWA
                     VSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGAAG
                     GGGGGGW"
     gene            1067..1939
                     /gene="rluB"
                     /locus_tag="ACHKG5_004306"
     CDS             1067..1939
                     /gene="rluB"
                     /locus_tag="ACHKG5_004306"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0870286.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(1979..2569)
                     /gene="cobO"
                     /locus_tag="ACHKG5_004307"
     CDS             complement(1979..2569)
                     /gene="cobO"
                     /locus_tag="ACHKG5_004307"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0870287.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(2566..3324)
                     /locus_tag="ACHKG5_004308"
     CDS             complement(2566..3324)
                     /locus_tag="ACHKG5_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0870288.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            3544..4593
                     /gene="sohB"
                     /locus_tag="ACHKG5_004309"
     CDS             3544..4593
                     /gene="sohB"
                     /locus_tag="ACHKG5_004309"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0870289.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(4629..4880)
                     /locus_tag="ACHKG5_004310"
     CDS             complement(4629..4880)
                     /locus_tag="ACHKG5_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0870290.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            5260..7857
                     /gene="topA"
                     /locus_tag="ACHKG5_004311"
     CDS             5260..7857
                     /gene="topA"
                     /locus_tag="ACHKG5_004311"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0870291.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1964 a         1858 c         2146 g         1946 t
ORIGIN      
        1 cctccacgtc atcgttgtta atttggtttc cgtgtctatt cctggtttgt ggatggttat
       61 atctctggaa gcgcaggccg caatttacgt cggtagatgt tttacggatg cagaaatttt
      121 atctgccgcg taaaaagtct tcgttttacc ggcctgatac ttttttgcaa tggggtggac
      181 tggcaatatt ggcggtcatt ctttacggta acctgagtcc tgtaggctgg gcaggaatga
      241 gtctggtggg cgatatgttt attatgatct gctggcttct tcctttttta ttttgttccc
      301 ttgagctttt gtttgcccgc gatgatgaca agccttgcgt taatcgtgta atcatcactt
      361 tgtttttacc gctgatttgt tcaggcgtgg ccttttattc tctctatatc aatgtcggag
      421 atgtattctt ttactggtat atgccagcgg gttattttag cgctgttttc ctgaccggtt
      481 atctcactgg catggggtat atttttctgc caaagtttac ccaaactggg cagcaacgtt
      541 atgcccacgg tgaagctatc gttaactatc tcgcgcgtaa agaggcagca acacacagtg
      601 ggcgtcggcg gaaaggggaa acacggaaac tggattacgc gttgctaggt tgggctgtct
      661 cagcaaacct tggaagagaa tgggtagcac gtatcacccc atcactcaca gcggctgttc
      721 gcgccccgga aattgcccgt agtggcgttt tgttctcatt acagatgcac ctgagtctgg
      781 gggccaatac cagtttattg gggcgaagtt attccggtgg tgctgcgggt ggcggaggcg
      841 gtggtggctg gtaattaagc tgatgttaat cgaaacaagc tttaaccata aagatagtgt
      901 agtatgttgc gcctcaaagc caggccagtt aaaacgtcga gtcgtttact taaggcctga
      961 agagttcaaa cagactttac tatcagtgag tcagatgtaa gttctgactc actgttgcac
     1021 aaaagcatat cagacgcctg ggaaggcgac accaaaagga agctctatga gcgaaaagct
     1081 acagaaagtg ctggcgcgtg ccggccacgg ttctcgccgt gaaatcgaat ctattattga
     1141 agccggtcgt gtgagtgttg atggcaaaat tgctaaactc ggcgatcgcg ttgaagttac
     1201 ccctggattg aaaatccgta tcgatggtca cctgatttcg gtacgtgagt ccgctgaaca
     1261 aatttgtcgc gttctggcct attataaacc ggaaggtgag ttgtgtaccc gtaacgatcc
     1321 ggaagggcgt ccaacggtgt ttgaccgtct gccaaagctg cgcggtgcac gctggattgc
     1381 cgtaggtcgt ctggacgtta acacctgtgg tctgttgctg ttcaccaccg acggtgaact
     1441 ggctaaccgt ttaatgcatc caagccgtga agttgaacgt gaatatgccg tgcgtgtatt
     1501 tggtcaggtt gacgacgcga aactgcgtga tttaagccgt ggtgtgcagc tggaagatgg
     1561 tccggcagcc tttaaaacca tcaagttcag cggcggcgaa gggatcaacc agtggtacaa
     1621 cgtgactctg accgaaggcc gtaaccgtga agttcgtcgt ctgtgggaag cggttggcgt
     1681 gcaggtaagc cgcctgatcc gtgttcgtta cggtgatatc ccactgccga aaggtctgcc
     1741 acgcggcggc tggacggagc tggatcttgc ccagactaac tatctgcgcg aactggtgga
     1801 gttgccgcca gaaaccagct ctaaagtcgc tgtagaaaaa gaccgtcgtc gcatgaaggc
     1861 gaatcagatt cgtcgtgcgg tgaaacgtca cagtcaggtg agcggcggac gtcgttctgg
     1921 tgggcgtaat aacggttaat caacgtatgc cggacgtcat atccggcatt tttacagatt
     1981 agtaatcaat ccctatctgc gcttttacgc cagcatcaaa cgcatgtttc acggggcgta
     2041 actcacttac cgtatccgcc agttcgagta tatcccgatg acagccccga cccgtgataa
     2101 tcaccgtctg ctgatgtgga cgttcattta acgcctgcaa cacttcttcc agtggcaaat
     2161 agtcatacgc caccatataa gtcagctcat caagcaaaac caaatccagt gaggaatcag
     2221 caagcatccg ctttgcatgt tgccagactt cgcggcaggc ggcggtatca gactcgcggt
     2281 tttgtgtatc ccaggtaaag cccgttgcca tcacctgaaa ctcaacgcca tgtggctcca
     2341 gcagattgcg ttcgccatta ggccaggtgc ctttaataaa ctgcacgacg cccacttttt
     2401 ttccgtgacc aactgcgcgt gttgccgtac caaatgccgc ggtggttttg ccttttccat
     2461 tgccggtaaa gacgatgata ataccgcgtt catcctgggc ctgggccata cgagcatcta
     2521 ctttttcttt cactcgctgc tgacgctgtt ggtagcgttc atcactcatt gggaaattcc
     2581 tggtttacgg cccggttggg cgtcaaaggt catgccggtt ttacggcggc tgtcatcgcc
     2641 catcagccag aggtagagcg gcatgatatc agcgggtgtt ttaagtttct gtggatcttc
     2701 ggtcgggaag gcgctggcac gcattgcggt gcgcgtaccg cctgggttaa tgcagttgac
     2761 acgcaggcgc tgctgatatt catcggccag tacctgcatc atcccttcgg tggcaaattt
     2821 cgacgctgca tatgcacccc agttggctcg tccctgacgt ccaacgcttg atgaagtaaa
     2881 gaccagtgaa ccggcgtccg atttgagtaa taaaggaagt agtgcctggg tgagcataaa
     2941 ggtggcatta acgttgacct gtatgacgtc ctgccagacc tgtggatttt gttcgctcat
     3001 tggacaaaca tcgccgagta atccggcatt atgcaaaaca ccatccagac gcggataatt
     3061 aacggcaatg cgctgtgcca gttgttggca atcttcggac ctgcaggtca gcaaatcgag
     3121 aataaaccac tgtggctgac gcccagtttc ttcgtttatg tggctggcta cctgacgtaa
     3181 tttttcttca ttacggccca acagaatcac tgtcgcacca tagcgtgcat acgtcatcgc
     3241 ggcttcacga ccaataccat cactggctcc cgtcaccagg ataatgcgat cattgagtaa
     3301 atcttgtttt ggctggtaat gcatggctac tcctcaacga cgttgtctgt cgcgctgcca
     3361 gtgtacttta tgactttggg gctttatgcc tgaaacagaa gggcatttca atcagcctcg
     3421 ggcgataaca ccgcaaaatg gatactttgt catactttcg ctgcaataac atctctgcga
     3481 gacggcttaa catgcctgtt gtaaactgtg agccaaagcg ttgtttaacc aaggtgggga
     3541 ctcgtggaat tgttgtctga atatggtttg tttttggcga aaatcgttac cgttgtgcta
     3601 gcgattgcgg cgattgccgc cattattgtc aatgttgctc aacgtaataa acgccagcgt
     3661 ggcgagttac gggtcaacaa tctcagcgaa cagtataagg agatgaaaga agaactggcc
     3721 gcggcgctga tggactcaca tcagcaaaaa cagtggcaca aagcgcagaa gaaaaagcac
     3781 aagcaagaag cgaaagcagc aaaagcgaaa gccaaactgg gcgaggtgtc aactgacagt
     3841 aaaccccgcg tctgggtgct ggactttaaa ggcagcatgg acgcccatga agtgaactcg
     3901 ctacgtgaag agataacggc ggtacttgca gcattcaaac cgcaggatca ggttgtgctc
     3961 cgtctggaaa gccctggtgg catggtgcat ggttacgggt tggcggcttc gcagctgcag
     4021 cgtctgcgtg ataaaaacat tccattaact gttacggtag acaaagttgc tgccagcggt
     4081 ggttacatga tggcctgtgt ggcggacaaa attgtttccg caccgtttgc tattgtgggt
     4141 tccattgggg tggtggcgca aatgcccaac tttaaccgct tcctgaaaag caaagatatt
     4201 gatatcgaac tgcacaccgc cgggcagtat aagcgtacgc tgaccttgct gggtgaaaat
     4261 accgaagaag ggcgggagaa attccgcgaa gagctgaacg aaacgcatca gttatttaaa
     4321 gattttgtga agcgtatgcg tccgtctctg gatattgaac aggtggcaac gggtgaacac
     4381 tggtacggac aacaggcggt agagaagggc ctggttgatg aaatcaacac cagtgatgaa
     4441 gttattctta gcctgatgga aggccgagaa gtggtcaatg tacgctatat gcagcgtaaa
     4501 cgactcattg accgattcac cggcagcgcg gcagagagcg ccgatcgatt attgctacgc
     4561 tggtggcagc gtgggcaaaa gccattgatg taaaagacaa acgcgagggt aagacctcgc
     4621 gttttgcttt aatcaaccag atgatatttt tctgaaagca catgggccag gtgtttgaac
     4681 atattaaaca ccgcggtgct tttggctgtt ggcaatcctt gttcatctaa aaagtagtcg
     4741 ccggtaaata ccagcacgcc attacgctgt gtgacgccgg tggcttcaat ccctgcgagc
     4801 gtatcctcat gctcacgaat gattttgttg gcctctttca acagcgtttc gcggtcgatg
     4861 ggttgtgtct ctttgttcat tatttactcc ttaaacaagg acattagtct acgccaggca
     4921 tggcttgcag acaaatatac cacgctggtg gcaagagcgc cttactggca actttggatt
     4981 ttgcatgcta ataaagttgc gtatcggatt ttatcaggta cagtgtgacg ctttcgtcaa
     5041 tctggcaata gatttgcttg acattcgacc aaaatttcgt cgtgctatag cgcctgtagg
     5101 ccaagacctg ttaactcagt cacctgaatt ttcgtgaaca gagtcacgac aaggggttga
     5161 tatccgcaga gagcgagtcc atatcagtaa ctcgttgcca gtggaaggtt tatcaacgtg
     5221 cgacgcattc ctggaagaat caaattaggt aaggtgaata tgggtaaagc tcttgtcatc
     5281 gttgagtccc cggcaaaagc caaaacgatc aacaagtatc tgggtagtga ctacgtggtg
     5341 aaatccagcg tcggtcacat ccgcgatttg ccgaccagtg gctcagctgc caaaaagagt
     5401 gccgactcta cctccaccaa gacggctaaa aagcctaaaa aggatgaacg tggcgctctc
     5461 gtcaaccgta tgggggttga cccgtggcac aattgggagg cgcactatga agtgttgcct
     5521 ggtaaagaga aggtcgtctc tgaactgaaa caactggctg aaaaagccga ccacatctat
     5581 ctcgcaaccg accttgaccg cgaaggggaa gccattgcat ggcacctgcg ggaagtgatt
     5641 ggtggtgatg atgcgcgcta tagccgagtg gtgtttaacg aaattactaa aaacgcgatc
     5701 cgccaggcat ttaacaaacc gggtgagctg aatattgatc gtgttaatgc ccagcaggcg
     5761 cgtcgcttta tggaccgcgt ggtggggtat atggtttcgc cgctgctatg gaaaaagatc
     5821 gctcgtggtc tgtctgccgg tcgtgtgcag tcggtggcag tccgcctggt ggtcgagcgt
     5881 gagcgtgaaa ttaaagcgtt cgtgccggaa gagttctggg aagtcgatgc cagcacgacc
     5941 acgccatctg gtgaagcgtt ggcgttgcag gtgactcatc agaacgacaa accgttccgt
     6001 ccggtcaaca aagaacaaac tcaggctgcg gtaagtctgc tggaaaaagc gcgctacagc
     6061 gtgctggaac gtgaagacaa accgacaacc agtaaacctg gcgctccttt tattacctct
     6121 acgctgcaac aagctgccag cacccgtctt ggatttggcg tgaaaaaaac catgatgatg
     6181 gcgcagcgtt tgtatgaagc aggctatatc acttacatgc gtaccgactc cactaacctg
     6241 agtcaggacg cggtaaatat ggttcgcggt tatatcagcg ataattttgg taagaaatat
     6301 ctgccggaaa gtccgaatca gtacgccagc aaagaaaact cacaggaagc gcacgaagcg
     6361 attcgtcctt ctgacgtcaa tgtgatggcg gaatcgctga aggatatgga agcagatgcg
     6421 cagaaactgt accagttaat ctggcgtcag ttcgttgcct gccagatgac cccagcgaaa
     6481 tatgactcca cgacgctgac cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt
     6541 attttgcgct ttgatggctg gacgaaagtg atgcctgcac tgcgtaaagg cgatgaagat
     6601 cgtatcttac ctgcagtcga taaaggcgat gctctgacgc tcgttgaact gacaccagcc
     6661 cagcacttta ccaagccgcc agcccgtttc agtgaagcat cgctggttaa agaactggaa
     6721 aaacgtggta tcggtcgtcc gtctacctat gcgtcgatca tttcgaccat tcaggatcgt
     6781 ggctatgtgc gagtagaaaa tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc
     6841 gatcgcctgg aagagaattt ccgcgagtta atgaactacg acttcaccgc gcagatggaa
     6901 aacagccttg accaggtggc aaatcacgaa gcagagtgga aagctgtact ggatcacttc
     6961 ttctcggatt tcactcagca gttagataaa gctgaaaaag atccggaaga ggggggtatg
     7021 cgtccgaacc agatggttct gaccagcatc gactgcccga cctgtggtcg caaaatgggg
     7081 attcgcacag cgagcaccgg ggtattcctt ggctgttctg gctatgcgct gccgccgaaa
     7141 gagcgttgca aaacaaccat taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa
     7201 ggcgaagacg ctgaaaccaa cgcgctgcgc gcaaaacgtc gttgccccaa atgcggcacg
     7261 gcgatggaca gctatctcat cgatccgaaa cgtaagttgc atgtctgtgg taataaccca
     7321 acctgcgacg gttacgagat cgaagagggc gaattccgca ttaaaggtta tgacggcccg
     7381 atcgttgagt gtgaaaaatg tggttctgaa atgcacctga aaatggggcg tttcggtaaa
     7441 tatatggcct gcaccaacga agagtgtaaa aacacgcgta agattttacg taacggcgaa
     7501 gtggctccac cgaaagaaga tccggtacca ttaccggagc tgccgtgcga aaaatcagat
     7561 gcctatttcg tgctgcgtga cggtgctgcc ggtgtgttcc tggcggccaa taccttcccg
     7621 aaatcgcgtg aaacgcgtgc gccgctggtg gaagagctgt atcgcttccg cgatcgtctg
     7681 ccggaaaaac tgcgttatct ggccgatgcg ccgcagcagg atccggaagg taataagact
     7741 atggttcgct ttagccgtaa aaccaaacag caatatgtct cttcggaaaa agacggaaag
     7801 gcgactggct ggtcagcatt ttatgttgat ggcaaatggg ttgaagggaa aaaataacct
     7861 ttaattctgt caggttttta taaacaaagg gtcgcgaaag cggccctttt ttat
//
LOCUS       DAZGXS010000177         7872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGXS010000177 DAZGXS010000000
VERSION     DAZGXS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..625)
                     /locus_tag="ACHKG5_004312"
     CDS             complement(<1..625)
                     /locus_tag="ACHKG5_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP0870292.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALA"
     gene            841..2274
                     /gene="cscA"
                     /locus_tag="ACHKG5_004313"
     CDS             841..2274
                     /gene="cscA"
                     /locus_tag="ACHKG5_004313"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0870293.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(2282..3277)
                     /locus_tag="ACHKG5_004314"
     CDS             complement(2282..3277)
                     /locus_tag="ACHKG5_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0870294.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            3520..3738
                     /locus_tag="ACHKG5_004315"
                     /pseudo
     CDS             3520..3738
                     /locus_tag="ACHKG5_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3756..5084
                     /gene="dsdA"
                     /locus_tag="ACHKG5_004316"
     CDS             3756..5084
                     /gene="dsdA"
                     /locus_tag="ACHKG5_004316"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP0870295.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(5192..6730)
                     /gene="emrY"
                     /locus_tag="ACHKG5_004317"
     CDS             complement(5192..6730)
                     /gene="emrY"
                     /locus_tag="ACHKG5_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0870296.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6730..>7872)
                     /gene="emrK"
                     /locus_tag="ACHKG5_004318"
     CDS             complement(6730..>7872)
                     /gene="emrK"
                     /locus_tag="ACHKG5_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0870297.1"
                     /translation="KKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVT
                     GNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTN
                     KLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAAL
                     NAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQ
                     VGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVT
                     GINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTK
                     NEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2155 a         1904 c         1826 g         1987 t
ORIGIN      
        1 ttgccagggc gcatatatcc cgatcgttct gtgtttttcc actgataagt cgccattctt
       61 cttccgagag cttgacgaca tccgccagtt gtagcgcctg ccgcaaacac aagcggagca
      121 aatgctcgtc ttgccataga tcttcacgaa tattgggatc gaagctgaca aaacctccgg
      181 catgccggat ctccgtcatc gcagtaaatg cgctggtacg cgaaggctcg gcagacaacg
      241 caattgaaca gagatgtaac cattcgccat gtcgccagca gggcaagtct gtcgtctcta
      301 aaaaaagatc ggcactgggg cggaccataa acgtaaatga acgttcccct tgatcgttca
      361 gatcgacaag caccgtggat gtccggtgcc attcatcttg cttcagatac gtgatatcga
      421 ctccctcagt tagcagcgtt ctttgcatta atgcaccaaa aggatcatcc cccacccgac
      481 ctataaaccc acttgttccg cctaatctgg cgattcccac cgcaacgtta gctggcgcgc
      541 cgccaggaca aggcagtagc cgcccgtctg attctggcaa gagatctacg accgcatccc
      601 ctaaaaccca tactttggct gacatttttt tcccttaaat tcatctgact tacgcatagt
      661 gataaacctc tttttcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca
      721 aaatgtcata gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact
      781 ttatttttat aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgataaaa
      841 atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca cgagcgccga
      901 ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccgggtggat gaacgatcca
      961 aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccaatgagt
     1021 gaacactggg ggccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag
     1081 catgagccta ttgcgctagc gccaggagac gagaatgaca aagatgggtg tttttcaggt
     1141 agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat
     1201 ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt
     1261 attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc
     1321 gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagaccca
     1381 ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tacgtgaatg gactttcgat
     1441 cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc
     1501 agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac
     1561 agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt
     1621 tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa
     1681 agctttgtag cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg
     1741 ccaatgccct caaaacgtga aggctgggca ggctgcatga cgctggcgcg cgagctatca
     1801 gagagcaatg gcaaacttct acaacgcccg gtacacgaag ctgagtcgtt acgccagcag
     1861 catcaatcta tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa
     1921 gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta
     1981 cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg
     2041 cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac
     2101 acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg
     2161 gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat
     2221 gcctcccacg gagtggctgt ggtgcaacat ggagcactct ggcaactggg ttaacataat
     2281 atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcacactt cccccttcac
     2341 gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg
     2401 tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg
     2461 cgacatcctg cgggatacgt acccccttag ccaggagaac ctgataagcc acaaaggctg
     2521 cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg aagtggtcat
     2581 tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca
     2641 cctcagccag atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc
     2701 ctgttgccaa cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga
     2761 gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg
     2821 ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga gactcaggta
     2881 gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca
     2941 gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc
     3001 agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtag
     3061 tagcagtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct
     3121 tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac
     3181 gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca
     3241 ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac
     3301 gggcactgta gcagaaacag acgtcactgg cgagatccaa cgaaattctc gacgccctat
     3361 cacccgacac ggcaatacaa taaaaaataa caataattgc cggataattg tacccaattc
     3421 cgcctctgtt ctcgccaacg agtgaccata taggcaagta agaaaaacat gccaactggc
     3481 atcgccaacc tggagaagaa aatataaata tggatcattg tgctgccgct gtatcccgac
     3541 atcagcccgg aaattattgc gattgctatc ggttcaggcg cgattggctg cacgatcgtt
     3601 acggactcgc ttttctggct ggtgaagcaa tattgcggcg ctacgctcaa tgaaacattt
     3661 aaatactata cgacagcgac atttatcgct tcagtcgtcg ctctggcggg cacattcctg
     3721 ctgtcattta tcatctaagc gcaaagagac gtactatgga aaacgctaaa atgaactcgc
     3781 tcatcgccca gtatccgttg gtaaaggatc tggttgctct taaagaaacc acctggttta
     3841 atcctggcac gacctcattg gctgaaggtt taccttatgt tggcctgacc gaacaggatg
     3901 ttcaggacgc ccatgcgcgc ttatcccgtt ttgcacccta tctggcaaaa gcatttcctg
     3961 aaactgctgc cactgggggg attattgaat cagaactggt tgccattccg gctatgcaaa
     4021 aacggctgga aaaggaatat caacaaccga tcagcgggca actgttactg aaaaaagata
     4081 gccatttgcc catttccggc tccataaaag cacgcggcgg gatttatgaa gtcctggcac
     4141 atgcagaaaa actggctctg gaagcggggt tactgacgct tgaagatgac tacagcaaac
     4201 tgctttctcc ggagtttaaa cagttcttta gccagtacag cattgctgtg ggctcaaccg
     4261 gaaatctggg gttatcaatc ggcattatga gcgcccgcat tggctttaag gtgacagtgc
     4321 atatgtctgc tgatgcccgg gcatggaaaa aagcgaaact gcgcagccat ggcgttacgg
     4381 tcgtggaata tgagcaagat tatggtgttg ccgtcgagga aggacgtaaa gcagcgcagt
     4441 ctgacccgaa ctgtttcttt attgatgacg agaattcccg cacgttgttc cttgggtatt
     4501 ccgtcgcagg ccagcgtctt aaagcgcaat ttgccgagca aggtcgtatt gtcgatgctg
     4561 ataaccctct gtttgtctat ctgccgtgtg gtgttggcgg tggtcctggt ggcgtcgcat
     4621 tcggacttaa gctggcgttt ggcgatcatg ttcactgctt ttttgccgaa ccaacgcact
     4681 ccccttgtat gttgttaggc gtccatacag gattacacga tcagatttct gttcaggata
     4741 ttggtatcga caaccttacc gcagcggatg gccttgcagt tggtcgcgca tcaggctttg
     4801 tcgggcgggc aatggagcgt ctgctggatg gcttctatac ccttagcgat caaaccatgt
     4861 atgacatgct tggctggctg gcgcaggaag aaggtattcg tcttgaacct tcggcactgg
     4921 cgggtatggc cggacctcag cgcgtgtgtg catcagtaag ttaccaacag atgcacggtt
     4981 tcagcgcaga acaactgcgt aatgccactc atctggtgtg ggcgacggga ggtggaatgg
     5041 tgccggaaga agagatggag caatatctgg caaaaggccg ttaataacgt ttcaacgcag
     5101 catcgcaatc ctttccctgg gtgagcgatg ctgccgatgg cgcagactta agatccccag
     5161 tcttacccgc tataaccccc tgcttttacc atcacccaac gcctttcgct gtaaacggcg
     5221 gtttcgcaaa ccaaaccaaa accgtaagta agataaaagc aatagccgcc atacggaaaa
     5281 tctcatttgc agaaatagaa agtgactgct gggttatttc attattaatt tcattaagaa
     5341 ctcctgaaag cgaaccatag tatttatcca taatttgtga cgatgaatta aacacggggt
     5401 taaattgatc gatggtttct gtcaactgac tatggtgtaa cgattcgcgt cgtccccaca
     5461 gcgtcattgt caacgacgta ccaactgatc ctgacaaggt acgaaaaaaa ttactcatac
     5521 tcgaggcatt ggcaaattta ttatctggca agcctgaaaa cgaaatcgtt gttaagggta
     5581 aaaagaaaca ggcaacggcg aatccctgaa aaaactgcgg caaaatgata cctgtaaaat
     5641 caatcgttgg cataaatgtc acagaacgcc agtaatagca aaccgcatac atcaaaaaac
     5701 tgaatgtcac caacacccgc atgtctattt tgttgccata acgtcctatc aaaggtgaaa
     5761 ttaatagtgg catgatgccg atgggcgcat aagcaagtcc ggcccatatc gcattatacc
     5821 ccatcgtttc ctggagtaac tgcggcataa ggacgatcgc tccagagtaa aataaatacg
     5881 cgcatgtgat actcacaata ccaatggtga agttacggga cttaaacaaa ctgagatcaa
     5941 gaatcgggtt ctctgaggtc gactcccaaa tgactaaaga gatcagaaaa ataactgata
     6001 ctactgttaa tattattatt gtactcgagt tgaaccaatc cagatcgcgc cctttatcaa
     6061 gcataatttg caagccacca acaccgagca ctaacagggt cagtcctggt agattcattt
     6121 tgaccggtga agtctcagtt tctcttcctt taagtaaggt taagcataat gtcaggacga
     6181 taatccccat agggacattg attaaaaata tccaacccca gctaaagtta tcacaaatat
     6241 aaccgcccaa tatcggccca catatcggag cgataatcac ggtcattgac cataatgcca
     6301 gagcaaatgt tcttttttct ggtggataat tccttaataa taaactctgt gacagtggaa
     6361 ttaacggccc tgccattaac ccctgaacga ctctaaaaaa tatcagcaca tcaagattgg
     6421 tcgataaact acacattaag gaagacagcg aaaaaaaagt gactgaaagt aaaaataacc
     6481 ttaattcgcc tattctttgt gccagcctgc cggtaacagg gatcgcaatg gcatttgcta
     6541 cgccaaacga ggtgataacc caggtgcctt cgtctgttga tgctcccaga aagccagata
     6601 ttgtcggtat tgcgacgtta gaaatagtgg aatccaacat ttgcataaac gtcgctaatg
     6661 acaatgcaat agtgacgcac cataacgtcc caccggttaa tggtgccgga gttgatttag
     6721 tgattgccat taaagttgtc cattatgcga aataatgttg ctaatttctt tttctatcgg
     6781 actggtatcg ataactaaag ccttactggt ataagccggc atggacgtca cggttgaagc
     6841 cagctcaggc atctcagcaa tgtcttcgtt cttcgtatca atagttgcag tcatcgataa
     6901 accaatgcgc aaggggtgtt ccatgagttc ttttggatca agagaaactt caaccggtac
     6961 acgctgaacg attttgatcc agttccctgt cgcattttgt gcaggtaata aggagaacgc
     7021 attgccggtt cccatattga tccctgtcac ccggccatga aacacaacat tctcaccata
     7081 aagatcgctg ataatattga ccgattgacc aatccgtaca tccgtgagtt gtgtttcttt
     7141 aaagttagca ttaacccaca tttgacgtgc cggtacgaca gccattaacg attgtccggg
     7201 actcactgtt tcgccgacct gaacacttct ctgggcaata tagccggtaa ccggactctt
     7261 aatatccgta cgtttaagcg ccaaccaggc ttctttggtt gcatccgcag cttcaacgac
     7321 ttgtggctga cggtttaatg gtgtgttcat tactaaagct ttattcgctt tataagcctg
     7381 gatagcggca ttcaatgccg ctttgctact tattaacgta tctttggtat gctccagcgt
     7441 ttcttttgaa ataaccccct gcttcgctaa aggcacacga cggttataat cttctaaaga
     7501 ttgttgatac tgaatacgtg ctgaagcgac ttcggcactg tattgtttat cctgtaagta
     7561 cagtttattc gtttgccgaa caatatttgc cagattattt ttagctttat tgagtgcgat
     7621 agtggcatca gttttatcca gtgaaactaa aatgtcaccc tgtcgaacgt agttcgtatc
     7681 tttatgatta acgacagtga cactacctga gacttgtgca gaaattggat ctgcattccc
     7741 cgtgacatag gcgtcatctg tactaatcat gtcttctaat tccattgacc aataggcata
     7801 agcacctgaa aacgcaataa ataaaactat cgccaataaa gaaaagtatt ttcttctgtt
     7861 agaatgtttt tt
//
LOCUS       DAZGXS010000178         7789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGXS010000178 DAZGXS010000000
VERSION     DAZGXS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(204..347)
                     /gene="ypaB"
                     /locus_tag="ACHKG5_004319"
     CDS             complement(204..347)
                     /gene="ypaB"
                     /locus_tag="ACHKG5_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0870298.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            475..2760
                     /gene="nrdA"
                     /locus_tag="ACHKG5_004320"
     CDS             475..2760
                     /gene="nrdA"
                     /locus_tag="ACHKG5_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0870299.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2987..4117
                     /gene="nrdB"
                     /locus_tag="ACHKG5_004321"
     CDS             2987..4117
                     /gene="nrdB"
                     /locus_tag="ACHKG5_004321"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0870300.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4117..4371
                     /gene="yfaE"
                     /locus_tag="ACHKG5_004322"
     CDS             4117..4371
                     /gene="yfaE"
                     /locus_tag="ACHKG5_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0870301.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4425..5075)
                     /gene="inaA"
                     /locus_tag="ACHKG5_004323"
     CDS             complement(4425..5075)
                     /gene="inaA"
                     /locus_tag="ACHKG5_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0870302.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5156..6346)
                     /locus_tag="ACHKG5_004324"
     CDS             complement(5156..6346)
                     /locus_tag="ACHKG5_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0870303.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6498..7376
                     /locus_tag="ACHKG5_004325"
     CDS             6498..7376
                     /locus_tag="ACHKG5_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0870304.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2095 a         2009 c         1830 g         1855 t
ORIGIN      
        1 aattaacaaa atggcttcgc atttaacaat aaccgaatag aaaacaacca tttcgacatc
       61 aacaatcgct tacattcgct tatatattga ccacaactga tacatcagat tatgtgatga
      121 ctcgtgctta gatcaatttt tgcaatcatt agcaaaaaga ttaataaacc atctatatca
      181 atttatccaa cctattctgc cgttcaagaa atcgccgaac agttatttat aacaaatttt
      241 tctcttccca ttgactttcc cggacacctt gtctgaccta aggtgcgcga aagccacttt
      301 ttccttcctg agttatccac aaagttatgc acttgcaaga gggccatttt cacactatct
      361 tgcagtgaat cccaaacata ccccctatat atagtgttct aagcagcttc ccgtactaca
      421 ggtagtctgc acgaaactat tgcggaaaga attccaaaaa caggtacgac atacatgaat
      481 cagaatctgc tggtgacaaa gcgcgacggt agcacagagc gcatcaatct cgacaaaatc
      541 catcgcgttc tggattgggc ggcagaagga ctgcataacg tttcgatttc ccaggtcgag
      601 ctgcgctccc acattcagtt ttatgacggt atcaagacct ctgacatcca cgaaaccatt
      661 atcaaggctg ccgcagacct gatctcccgt gatgcgccgg attatcagta tctcgccgcg
      721 cgcctggcga tcttccacct gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg
      781 tacgaccacg tggtgaaaat ggtcgagatg ggcaaatacg ataatcatct gctggaagac
      841 tacacggaag aagagttcaa gcagatggac acgtttatcg atcacgaccg tgatatgacc
      901 ttctcttatg ctgccgttaa gcaactggaa ggcaaatatc tggtacagaa ccgtgtgacc
      961 ggcgaaatct acgaaagcgc ccagttcctt tatattctgg ttgctgcgtg cctgttctcg
     1021 aactacccgc gtgaaacgcg cctgcaatat gtgaagcgtt tttacgacgc ggtttccaca
     1081 tttaaaattt cgctgccgac gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc
     1141 agctcctgcg tactgatcga gtgcggcgac agcctggatt ccatcaacgc cacctccagc
     1201 gcgattgtga aatatgtttc ccagcgtgcc gggatcggca tcaacgccgg gcgtattcgt
     1261 gcgctgggta gcccgattcg cggtggcgaa gcgttccata ccggctgcat tccgttctac
     1321 aaacatttcc agactgcggt gaaatcctgc tctcagggcg gcgtgcgcgg cggtgcggca
     1381 acgctgttct acccgatgtg gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac
     1441 cgtggtgtgg aaggcaaccg cgtgcgtcat atggactacg gggtacaaat caacaaactg
     1501 atgtataccc gcctgctgaa aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg
     1561 gggctgtacg acgcattctt cgccgatcag gaagagtttg aacgcctgta taccaaatat
     1621 gagaaagacg acagcatccg caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg
     1681 atgcaggaac gtgcgtctac cggtcgtatc tatattcaga acgttgacca ctgcaatacc
     1741 catagcccgt ttgatccggc catcgcgcca gtgcgtcagt ctaacctgtg cctggagata
     1801 gccctgccga ccaaaccgct gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt
     1861 acgctgtctg ctttcaacct gggcgcaatt aatagcctgg atgaactgga agagctggca
     1921 attctggcgg ttcgtgcact tgacgcgctg ctggattatc aggattaccc gatcccggcc
     1981 gccaaacgtg gagcgatggg tcgtcgtacg ctgggtattg gtgtgatcaa cttcgcttac
     2041 tacctggcga agcatggtaa acgctactcc gacggcagcg ccaacaacct gacgcataaa
     2101 accttcgaag cgattcagta ttacctgctg aaagcgtcta atgagctggc gaaagagcaa
     2161 ggcgcgtgcc cgtggtttaa cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc
     2221 tataagaaag atctggatgc catcgctaat gaaccgctgc attacgactg ggaagctctg
     2281 cgtgagtcaa tcaaaacaca cggcctgcgt aactccacgc tttctgctct gatgccgtcc
     2341 gagacttctt cgcagatctc taacgccact aacggtattg aaccgccgcg cggttacgtc
     2401 agcatcaaag cgtcgaaaga cggtattctg cgccaggtgg tgccggacta cgagcatctg
     2461 cacgacgcct atgagctgct gtgggaaatg ccgggtaacg atggctacct gcaactggtg
     2521 ggtatcatgc agaaatttat cgatcagtcg atctctgcta acaccaacta cgatccgtca
     2581 cgcttcccgt caggaaaagt gccgatgcag cagttgctga aagacctgct caccgcctac
     2641 aaattcgggg tcaaaacgct gtattatcag aacacccgtg acggcgctga agacgcgcag
     2701 gacgatctgg tgccgtcaat ccaggacgat ggctgcgaaa gcggcgcatg taagatctga
     2761 tattgagatg ccggatgcgg cgtaaacgcc ttatccggcc tacggctcgg tttgtaggcc
     2821 tgataagacg cgccagcgtc gcatcaggca caggatgcgg cgtgaacgcc taatccggcc
     2881 tacggctcgg atttgtaggc ctgataagac gcgccagcgt cgcatcaggc acaggatgcg
     2941 gcgtaaaatg ccttatccgg cattaaactc ccaacaggac acactcatgg catataccac
     3001 cttttcacag acgaaaaatg atcagctcaa agaaccgatg ttctttggtc agccggtcaa
     3061 cgtggcccgt tacgatcagc aaaaatatga catcttcgaa aagctgatcg aaaagcagct
     3121 ctctttcttc tggcgtccgg aagaagttga cgtttctcgt gaccgtatcg attaccaggc
     3181 gctgcctgaa cacgaaaaac acatctttat cagcaacctg aaatatcaga cgctgctgga
     3241 ctccattcag ggtcgtagcc cgaacgtggc actgctgccg ctgatctcga tcccggaact
     3301 ggaaacctgg gtagaaacct gggcgttctc agaaacgatt cattcccgtt cctatactca
     3361 tatcattcgt aatatcgtta acgatccgtc tgttgtgttt gacgatatcg tcaccaacga
     3421 gcagatccag aaacgtgcgg aagggatctc cagctactac gatgagctga tcgaaatgac
     3481 cagctactgg cacctgctgg gcgaaggcac ccacactgtt aacggtaaaa ccgtgaccgt
     3541 tagcctgcgc gagctgaaga aaaaactgta tctctgcctg atgagcgtta acgcgctgga
     3601 agcgatccgc ttctacgtca gctttgcttg ttcctttgcc tttgcagaac gcgaactgat
     3661 ggaaggtaac gccaaaatta ttcgcctgat tgcccgcgac gaagccctgc acctgaccgg
     3721 tactcagcat atgctgaatc tgctgcgcag cggtgcggat gatccggaga tggcggaaat
     3781 tgccgaagaa tgtaagcagg agtgctatga cctgtttgtc caggcggcac agcaagagaa
     3841 agactgggcg gattatctgt tccgtgacgg ttcgatgatt ggtctgaata aagacattct
     3901 ctgccagtac gttgaataca tcaccaatat ccgcatgcag gcagtcggtt tggatctgcc
     3961 gttccagacg cgctccaacc cgatcccgtg gatcaacacc tggctggtgt ctgataacgt
     4021 gcaggttgct ccgcaggaag tggaagtcag ttcttatctg gtcgggcaga ttgactcgga
     4081 agtggacacc gacgatttga gtaacttcca gctctgatgg ctcgcgttac cctgcgcatc
     4141 actggcacac aactgctgtg ccaggatgaa cacccttccc ttctggcggc gctggaatcc
     4201 cacaatgtgg cggttgagta ccagtgccgc gaaggttact gcggctcctg tcgcacacgc
     4261 ctggttgcag gccaggtgga ctggattgcc gaaccgttag cctttattca gccgggggaa
     4321 attttgccct gttgttgccg agcaaaaggc gatattgaaa tcgagttgtg atcgggtact
     4381 agcgccagat acgacgctta cgcgtattat ctggcctaca acgattacat ggcgtagtaa
     4441 tacgctttta cctgctccca gtccgctttg gggatcggct ccagatattt ttccagctgg
     4501 cggaagtcat gattaatcgc tttatcgcga cgcaagcggc gacgactttt ctccagatca
     4561 agaaaaccag cttctgcctt accttctgtt ttcacataga tatggcgaac ataacaacag
     4621 ccatgctgac gattaacgct atgcattttc ttaaacgcca gcgccactgc tttcaacatg
     4681 gcttgccgta cttcgtcaga atagggcgtt actgcatgct gggcatacca gtcagcaatg
     4741 ctgatgaacc ccgccatatc ttcagtcacc agcaacgcgc gccattcccc ctcaattttc
     4801 accgcttcac caaaaacgat cttcggcacg atgacaccgg cccgttccag ttctttaatt
     4861 accgcaacct cacggacaat cgttggtcgg ccgaaaggat aacgtacgga atgaaacaga
     4921 tgatgcgtca tgcgctttac atatagcttt ttgccgttgc gctcgacgca ttgcaccccg
     4981 ctcataccat tacggcgata gttaggctct tcaacccagt cgccctctgt tgcccaccag
     5041 tgattaaatt cgtcgtgctt tgctgaaact gccataccct atcgcctgtc attttttatt
     5101 aacgacaatg actataggtg gttacctgcg aaaaatctta atgaaacgtg tcgtattaat
     5161 gaatttcttt tgccactact agcgcgacac cgctttttcc cttcatttgc agcgcataga
     5221 ctccaccagc cactaccact aatagtgcgc ccagcagata aatcattcgg tagccagtat
     5281 gtgcaaccat catccccatc acacaggctc ccagcgccag gcctgcatcc atcgcattga
     5341 agaagaggga gtttgcgaca ccgattttat gcggttccac tgaactgata atctgcgtct
     5401 gaaaaactgg cgtcacggaa ccatacccga taccaatcaa cgcgccagaa acgaccatca
     5461 ttacgctgcc ctgggtgtag ccgagaacca ccagcccaac cgtaaagaac agcaggcagg
     5521 ggtaaaccac atatttcggg ccttttttgt cgcaaacatt gccggtgaag gtacggcaga
     5581 tcatcaggaa gatggcgtag cagagcagga aattactggc cgccgacatc agattaagtt
     5641 cgcgggcgta aagcgccaga aaggcagaaa ccccggcata cgaaaaggtc ataaagaatg
     5701 taaccatggc aaacggcaat gccgcgcgat cgaacatggc ggcgaagccc atctttggct
     5761 ttgaaccgtc ggcatggctg ataaccggcg gcacggtcat aatcagcgac aggataatcc
     5821 ccaccgccgc aacgccagta cataaccaga aagccatatt aaaggcctcc cagcgcgcca
     5881 tattcaggcc aatccacggc ccgaccacca ttgccagccc cattgccaga gagaaaaaac
     5941 tgatgccctc cccacgtcgg gaggccggga tcagccgcgc ggaaatcgtt ccctttaccg
     6001 ttgtgataac cccgaaggta ataccatgaa gcaccctaat aaaaagcagt gattctattg
     6061 agtggcaaaa aggatacagc gcagtgacca ccaaaaaggc cagagaagag agcaccagaa
     6121 tagttttatt cgaatattta cccacccatt gcccggcaaa gggtcgaata acaatcgccg
     6181 caattaaaaa taaagtcacc aacaaacctg ctttatcagg agaggcatgc aagcggtcag
     6241 agatataaat tggcaacaat gttagtaaaa cataaaagac gaagaaaata ataaagctga
     6301 tgatggttat tgcccaaaaa tccttcgtcc ataacttctc tttcatactc taccgcctgt
     6361 caggatgacg cacgcagaca acactcccgt tactgcggtt gcgtcaaatt gtgatgtact
     6421 ttgcagaaca gaagtataaa tggcggcatg ctattagaga aattaatcgt tttaatatta
     6481 ttgattagca aactctaatg actcttaccc aaataaattc tttactcgcc gtgctggatt
     6541 acggtggctt tactgaagcc agcaagcgat tgttcatgac gcagtcggcg gtaagtcagg
     6601 caattgctgc cctcgaacaa gaacttggtg tgaatatact gttacgcgat cgccgtaaag
     6661 ctatacaact gaccacagca ggtcaccgca ttgtgcaaca tctgcgcgcc attaatcggg
     6721 aagttaacgc agtaaaagag atagccgagc aggaaaagca gaaccctcaa agaacgctga
     6781 gacttggttg tttccccagc gtctgcgcct gtatcttacc ggttgtgatt cgctactttg
     6841 aaattcatca tccgaatata aaaattattc cttttgagga aaatagcaca gcgattattg
     6901 attcgcttca gaatgaatcc attgatgccg gttttgttca ttttccggtg agcggaatgt
     6961 actccgtgcc catttatcag gataaattca ccgtagtgtt accgcccaac catgctctgg
     7021 caaaaaacag cactattaca gtggaagaat taatgggtga accattaatc attagtaaag
     7081 gacgctatga attaagcatt atggcactat ttaaagaaaa gaatattacc ccacagataa
     7141 aatatgaatt taatcacccg gatacagcga tcagttttat tcgacaggga ttaggcatcg
     7201 ccttattacc agagttaacg ctgaaaacca ttgcagatga attgtgttcc gttcctctcg
     7261 aaccaacctt ctatcgacag atttcgttgc tggctaaaga aaagccggta gaaggcagtc
     7321 cactgttttt actacaaatg tgtacggaac aactagtggt gagtggaaaa atttgatgta
     7381 agacatgaga ttgatgacaa atgcaagact gcctgattcg ccacacttag ttgtactata
     7441 gcctttacca ggcgtaataa ttcgttttca cctgattttg gcccactttg tgaattggcg
     7501 atatatttat ccagttggga gaagtgacaa tgactccagg tagaaaccgg tatgaagcgc
     7561 ccgactcact gtcaaaaccg attgttgaga gtaatgacac tccggcgaac agtacacagt
     7621 ttactctgga gccattctca atcttcaagt gggataccta ataacttgtc agggaagtta
     7681 tcaatatctg tttgttactt attaccgtat ttattaacaa taatgatact ttctaacatg
     7741 cgtaaaggtc ttcgtaataa aatgagctaa atttgatagt gaatatatt
//
LOCUS       DAZGXS010000179         7632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGXS010000179 DAZGXS010000000
VERSION     DAZGXS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            128..1525
                     /gene="ulaA"
                     /locus_tag="ACHKG5_004326"
     CDS             128..1525
                     /gene="ulaA"
                     /locus_tag="ACHKG5_004326"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0870305.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1541..1846
                     /gene="ulaB"
                     /locus_tag="ACHKG5_004327"
     CDS             1541..1846
                     /gene="ulaB"
                     /locus_tag="ACHKG5_004327"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0870306.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1856..2320
                     /gene="ulaC"
                     /locus_tag="ACHKG5_004328"
     CDS             1856..2320
                     /gene="ulaC"
                     /locus_tag="ACHKG5_004328"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0870307.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2334..2984
                     /gene="ulaD"
                     /locus_tag="ACHKG5_004329"
     CDS             2334..2984
                     /gene="ulaD"
                     /locus_tag="ACHKG5_004329"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0870308.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2994..3848
                     /gene="ulaE"
                     /locus_tag="ACHKG5_004330"
     CDS             2994..3848
                     /gene="ulaE"
                     /locus_tag="ACHKG5_004330"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0870309.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3848..4534
                     /gene="ulaF"
                     /locus_tag="ACHKG5_004331"
     CDS             3848..4534
                     /gene="ulaF"
                     /locus_tag="ACHKG5_004331"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0870310.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4663..4938)
                     /gene="yjfY"
                     /locus_tag="ACHKG5_004332"
     CDS             complement(4663..4938)
                     /gene="yjfY"
                     /locus_tag="ACHKG5_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0870311.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5265..5660
                     /gene="rpsF"
                     /locus_tag="ACHKG5_004333"
     CDS             5265..5660
                     /gene="rpsF"
                     /locus_tag="ACHKG5_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0870312.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5667..5981
                     /gene="priB"
                     /locus_tag="ACHKG5_004334"
     CDS             5667..5981
                     /gene="priB"
                     /locus_tag="ACHKG5_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0870313.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5986..6213
                     /gene="rpsR"
                     /locus_tag="ACHKG5_004335"
     CDS             5986..6213
                     /gene="rpsR"
                     /locus_tag="ACHKG5_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0870314.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6255..6704
                     /gene="rplI"
                     /locus_tag="ACHKG5_004336"
     CDS             6255..6704
                     /gene="rplI"
                     /locus_tag="ACHKG5_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0870315.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6775..7569)
                     /locus_tag="ACHKG5_004337"
     CDS             complement(6775..7569)
                     /locus_tag="ACHKG5_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0870316.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPSYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1836 a         1853 c         2069 g         1874 t
ORIGIN      
        1 attatttgcg ggtcgcgtca catttaatca taaataatct tgttgtgatt acttttgaaa
       61 attagagtga gtgcacaaca ttccgggtgt gtggaatacc cggttacctc ttcttcagga
      121 gatcgttatg gagatcctct acaacatctt taccgtattt tttaaccagg tcatgaccaa
      181 tgccccgttg ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt
      241 cagcgttatt attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg
      301 gtccggcatc ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg
      361 cattaacggc gcaatttccg atacctacgc ttcaatgatg gcaaccatcg accgcatggg
      421 cgatgcctat agctgggtgg gttacgccgt attgttagcg ctggcactga acatctgtta
      481 cgtgctgttg cgtcgcatta ccggcattcg cacaatcatg ttgaccggcc acatcatgtt
      541 ccagcaggcc gggttgattg ccgttacgct gtttatcttc ggctactcca tgtggaccac
      601 cattatctgt accgcgattc tggtttcgct ctactggggc atcacttcca acatgatgta
      661 caagccgact caggaagtga cggatggctg tggtttctcc atcggtcacc agcagcagtt
      721 tgcatcatgg attgcctata aagtcgcgcc gttcctcggc aaaaaagagg agagcgttga
      781 agacctcaaa ctgccgggct ggctgaacat tttccacgac aacatcgtct ccacggcgat
      841 tgtgatgacc atcttctttg gcgccattct gctctccttc ggtatcgaca ccgtgcaggc
      901 gatggcgggc aaagtgcact ggacggtcta catcctgcaa accggtttct ccttcgcggt
      961 ggcgatcttc atcatcactc agggcgtgcg catgtttgtg gcggaactct ctgaagcatt
     1021 taacggtatc tcccagcgcc tgatcccagg tgcggttctg gcgattgact gtgcggctat
     1081 ctatagcttc gcgccgaacg ccgtggtctg gggctttatg tggggcacca tcggtcagct
     1141 gattgcagtt ggcatcctgg tcgcctgcgg ctcttccatt ctgattattc caggctttat
     1201 cccgatgttc ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg
     1261 tgcagcgctg aagatttgtc tggtgatggg aatgatcgaa atctttggtt gcgtctgggc
     1321 ggtgaaactc accggtatga gtgcctggat gggcatggcg gactggtcga ttctggcacc
     1381 gccgatgatg caaggcttct tctccatcgg tatcgccttt atggccgtca tcattgtaat
     1441 tgcactggct tatatgttct tcgctggccg cgcgctgcgc gcagaagaag atgcagaaaa
     1501 acaactggca gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc
     1561 tgtgtgtggc aacggacaag gcagttccat gatcatgaag atgaaagtgg accagttttt
     1621 aacccaatca aacattgacc atacggtaaa cagctgcgcg gttggcgagt acaaaagcga
     1681 gttgagtggc gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac
     1741 cggcaacaaa tacgtggttg gcgtgcgcaa catgctctct cctgccgact ttggcccgaa
     1801 actgctggaa gtgatcaaag agcatttccc gcaggacgtg aagtaaggac gcgccatgaa
     1861 attacgtgat tcgctggctg aaaataaatc catccgtctg caggctgaag cagagacatg
     1921 gcaggaagcg gtgaaaatcg gcgttgacct gctggtggcg gcagatgtgg tagagccgcg
     1981 ttactaccag gcgattctgg atggcgttga acagttcggt ccttatttcg ttatcgctcc
     2041 gggcctggca atgccgcacg ggcgtccgga agagggcgtt aagaaaaccg gtttctctct
     2101 ggtaacgctg aaaaagccgc tggagttcaa ccacgatgac aacgatccgg tggatatcct
     2161 catcaccatg gcggcggtcg atgccaatac tcaccaggaa gtgggcatca tgcagatcgt
     2221 caacctgttt gaagatgaag agaattttga ccgtttacgc gcctgccgta ccgagcagga
     2281 agtactggat ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aacatgtcat
     2341 taccgatgtt gcaagtcgcg ctggacaacc agactatgga tagcgcctac gaaaccactc
     2401 gcctgattgc cgaagaagtc gacattatcg aagtgggcac cattctgtgc gtgggcgaag
     2461 gcgtgcgtgc ggttcgtgac ctgaaagcgc tctacccgca caaaatcgta ctggcagacg
     2521 ccaaaattgc cgatgcaggc aaaatccttt cgcgtatgtg cttcgaagcc aacgctgact
     2581 gggtgacggt aatttgctgt gcggatatca acaccgccaa aggcgcgctg gacgtggcaa
     2641 aagagtttaa cggcgacgtg cagatcgaac tgaccggtta ctggacctgg gaacaggcgc
     2701 aacagtggcg cgacgcaggt attcagcagg tggtttatca ccgcagccgt gacgcgcagg
     2761 ccgcaggcgt ggcgtggggc gaagcggaca tcaccgcgat caaacgtctt tccgatatgg
     2821 gcttcaaagt caccgtcact ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca
     2881 ttccgattca cgtctttatc gcgggccgta gtatccgtga tgccgcttct ccggtggaag
     2941 ccgcacgtca gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg gatatgttgt
     3001 ccaaacaaat cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg
     3061 aacgcctgca actggcaaaa acgttgggct tcgattttgt cgagatgtcg gtagatgaaa
     3121 cggacgagcg cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg ctggtgaatg
     3181 cgattgttga aaccggcgtg cgcgtgccgt ccatgtgcct ttctgctcat cgtcgtttcc
     3241 cgctgggcag tgaagatgac gcggtgcggg cgcaggggct ggagattatg cgtaaagcta
     3301 tccagttcgc ccaggacgtt ggtattcgcg tgatccaact ggcgggctat gacgtttact
     3361 atcaggaagc caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg
     3421 agatggcgag ccgcgcgcag gtgacgctgg cgatggagat catggattat ccgttgatga
     3481 actccatcag caaggcgctg ggctacgcgc actatctcaa caatccgtgg ttccagctct
     3541 acccggatat cggcaacctg tcggcgtggg acaacgatgt gcagatggag ttgcaggccg
     3601 gaatcgggca tatcgtcgcg gtacatgtga aagacaccaa accgggcgtc ttcaaaaacg
     3661 tgccatttgg cgaaggtgtg gtggatttcg aacgttgctt cgaaacgctc aaacagagtg
     3721 gctattgcgg gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag
     3781 aagtggcgaa agcgcgtgat tgggtgaaag cgcgcatggc gaaagcgggc atggtggagg
     3841 cggcataatg caaaagctaa aacagcaggt gtttgaagcc aacatggatc tgccgcgcta
     3901 tgggctggtg acctttacct ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt
     3961 ggtgatcaag cccagcggcg tcgcctacga aaccatgaaa gcagacgata tggtggtggt
     4021 cgatatgagc ggtaaggtgg tggaagggga atatcgtccg tcttccgaca ccgcgacgca
     4081 tctcgaactc taccgtcgtt atccgtcgct tggcggcatt gtccataccc actccactca
     4141 tgctaccgca tgggcgcagg cggggctggc gatcccggcg ttaggcacca cgcacgctga
     4201 ctacttcttt ggtgatatcc cgtgtacgcg tggattaagc gaagaagagg tgcagggtga
     4261 gtatgaactg aacactggca aagtgattat cgaaacgctg ggcaacgccg agccgctgca
     4321 tacgccggga attgtggtgt atcagcacgg accgttcgcc tgggggaaag atgcccacga
     4381 cgcggtgcat aacgcggtag tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag
     4441 tattaatcca caactcaatc acatcgacag cttcctgatg aataaacact tcatgcgtaa
     4501 gcacggcccc aacgcttatt acgggcagaa gtagaacacg cgctgcggaa atttccttcc
     4561 tcgggagata actggtctaa ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg
     4621 gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc
     4681 gctggcgtgc atattgccgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
     4741 tgcgccttgc gcatctgttt cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa
     4801 attatgattg atataaatca cgcctaagct ttgcacatcg tccatatttc tggcctggtg
     4861 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
     4921 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
     4981 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
     5041 cagttgttcg aaaaaacacc atgatgaagt gtgatgaact tcaaatcagc gtgttagtcc
     5101 actttggtga aagatgagat ttatttgtcg gctggccttg agtttagcgg ggtatgtcag
     5161 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
     5221 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
     5281 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
     5341 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
     5401 agctggctta cccgatcaac aaactgcaca aagctcacta cgttctgatg aacgttgaag
     5461 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
     5521 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
     5581 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
     5641 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
     5701 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
     5761 agcatcgttc tgtgcaggag gaagccggct ttcaccggca ggcatggtgt caaatgcccg
     5821 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
     5881 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
     5941 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
     6001 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
     6061 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
     6121 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
     6181 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
     6241 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
     6301 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtacca cagggtaaag
     6361 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
     6421 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
     6481 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
     6541 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
     6601 gtctgccgaa cggcgttctg cgtaccactg gcgaacacga agtgagcttc caggttcaca
     6661 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
     6721 acgtaaaaac gccgaccact ggtcggcgtt ttgctttcta tttttcgtca ggtattagtt
     6781 tcgcaagtag atccattcac cattctcttg tgtccataag tcaggatggt ttgctggcgt
     6841 tagatacata cgagcgcaac tgatgtctcg ctcctgcggc actcgctcga aacccaaacc
     6901 cgagtagatt gcggctgaac ttgataagga acgcagacca attttcaaat catggggtaa
     6961 atcagaatac gcgtttagca atgcagcaat gatgaatccg agggcattag aaacgcctgt
     7021 aacagtgcga agttggtcga tataaagatc gattgttggt agttcagatg accagcaatt
     7081 aggcatttgg gcaggaaggg ttacaacgta tcctatcgtt tggccggtat agtctggagt
     7141 accataacta ggtcccaaaa ccacttcgtg tattgtgtat ttactgttgg gttcgaagga
     7201 ttcgacatct tgcaataaat atttcagttc tgcggttaac ctcgcacccg cgtaagcttt
     7261 attgtatttc tgggcgtgaa tattatctaa tgtagcttca tggttccatc caaaagaatt
     7321 atcaccattc attgttactc catcgtaagc attacacttc gttgctaact tattgaatgc
     7381 ttgccagtct gagagatcta gtttgtggat ctcaacagta cgtctgccat cgcaattatg
     7441 atctcttcca cgatttgaaa gtaatgtagc gcgtgcgtat tgatgaaatt ttaattttac
     7501 tggtttgtcg gtggacgaaa aacgagataa atatgcagga aatgaataaa tagtggttgg
     7561 taatgtcatt gtaatgtgac ctgtttaatg tttttataaa tatcgcagct gaatgttcac
     7621 agaaaaaaat cg
//
LOCUS       DAZGXS010000180         7505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGXS010000180 DAZGXS010000000
VERSION     DAZGXS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..91)
                     /locus_tag="ACHKG5_004338"
     CDS             complement(<1..91)
                     /locus_tag="ACHKG5_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870317.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDD"
     gene            complement(228..2330)
                     /locus_tag="ACHKG5_004339"
     CDS             complement(228..2330)
                     /locus_tag="ACHKG5_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0870318.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEATPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(2330..2821)
                     /locus_tag="ACHKG5_004340"
     CDS             complement(2330..2821)
                     /locus_tag="ACHKG5_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0870319.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(3496..3648)
                     /locus_tag="ACHKG5_004341"
     CDS             complement(3496..3648)
                     /locus_tag="ACHKG5_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870320.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(3636..4103)
                     /locus_tag="ACHKG5_004342"
     CDS             complement(3636..4103)
                     /locus_tag="ACHKG5_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0870321.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(4100..4597)
                     /gene="rrrD"
                     /locus_tag="ACHKG5_004343"
     CDS             complement(4100..4597)
                     /gene="rrrD"
                     /locus_tag="ACHKG5_004343"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0870322.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(4597..4812)
                     /locus_tag="ACHKG5_004344"
     CDS             complement(4597..4812)
                     /locus_tag="ACHKG5_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0870323.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            4955..5353
                     /locus_tag="ACHKG5_004345"
     CDS             4955..5353
                     /locus_tag="ACHKG5_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870324.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(5434..5592)
                     /locus_tag="ACHKG5_004346"
     CDS             complement(5434..5592)
                     /locus_tag="ACHKG5_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0870325.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(5678..6421)
                     /locus_tag="ACHKG5_004347"
     CDS             complement(5678..6421)
                     /locus_tag="ACHKG5_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870326.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(6605..7294)
                     /locus_tag="ACHKG5_004348"
     CDS             complement(6605..7294)
                     /locus_tag="ACHKG5_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0870327.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(7309..7431)
                     /locus_tag="ACHKG5_004349"
     CDS             complement(7309..7431)
                     /locus_tag="ACHKG5_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0870328.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT         2001 a         2019 c         1606 g         1879 t
ORIGIN      
        1 catcatcaag aattgccatc agaacctcgc cagccggtac ccgggatgcc cccgtcgtcg
       61 tgtaatcaga gccgcaaggc ggcgctccca ctcctgccgt ccctgccgga tctcagataa
      121 gttttccatg gtcatctgct gaccattaaa ggtgacggat tttccgtcca gcaccgccat
      181 ttcagcttcc gtataacgct gaatcatggc ttcgatatca ttctggttca taaccatcct
      241 ccggaagtca gccaggggtt aacatcgtca gttactgttt tcttccgttt ttgtttttta
      301 acaggcgtgg ataccggttc cggtgagggt gacggttcgg tactgtccgg gacactctcc
      361 agccaggttt cccggctcgc ccaccccggt gcatccggcc agcggatctt ttcgtatcca
      421 tgcagaatga ccagagcctc ggcatacacc atcaggtcaa aagcttcgtt ggcaccgcga
      481 cccggcttac tccatttccc gtcactactc cgctcttcat acgtcagttc gtcgtaaaac
      541 cagctcccca gccagtcagg gaaatgcaca tagccgggac ctggagagtc acgccataac
      601 gcgttattca cccggtcttt cagtgcatcc gtctgaagaa gccagagcgg cacatcacct
      661 gcggcctgcg cccgtcggcc cgttcgtccg gtgttatcag ggaatgtacg ggtgatcagt
      721 tttgcgcgcc ggatgctgtc gcccttaaac aggtaaatac gtttaccaag gccatcacga
      781 cggcaacgac gccagaattt ataggcatta tcagtgaccc cgtcttcacc gccggagtcc
      841 accgccattg ccatcagtcg catttgttga gaaggatcgg aggccagcgg ccagctttta
      901 tgaaaaacat ccgtcagcag gacatcccag tcttccggat agctggccgg atcaattcgc
      961 tggctctcac cgtcgctgtc accgcgcaat gactgcgtga tgttgtaacg atcaataatc
     1021 cagcgttcgc cacggctgcc atagcccgtt acctgaacca caaaacggcg atgacgtccc
     1081 gcctgcacat ccactgtcgc cacaaggaaa ttaacgccat ccggcacact gctggaagga
     1141 actggctctg cccgctgctc aagcagttca ctttttcgtt gctccatgct ggcgcgggga
     1201 agataaggta atccccagtc ggtattgata accgtcttga gtgtttcttc acttccggtt
     1261 gtctcgtatt cctgttctgc agtaagcagt ttgtaaacga gttgcgagag tgtctggtaa
     1321 gcagctgccg gaccctccat ccagaatgac gcaatacgtg agcgtcgggg atcaccataa
     1381 cgactaccat ccgcattgat ggattcacca tcccgcaacc agaccccacg tccgttcagc
     1441 tcacgttttt gttcaggcat aatccgtcct gaacaggaag gacactgaat ataagccgcc
     1501 tcacttgcca gcacgggatc ggcaatatca cggaaaccag caaccacatc gccgcagggc
     1561 tgaaaatact caccacagtg tggacagggc cagtaccagc gacggcgatc gccacggtta
     1621 tagagcgaca gtatccccgt ggttggtgta gcctcatgcg gtgaagtccg tcgccatttc
     1681 acatccttca catccctgcc gggggaactc tccaccagcg tcataccact ggacataaat
     1741 gtggtggtac gttttgaggc aagagagaag gcatccccct cgccatcaat atcttccgga
     1801 aaacggtcat aatccgtcag cgccacgcat ttataatctg atgaggacat gatattgact
     1861 gacggccagc cgattttcag gtagttgcca gcaaggaatg ttctgtcata aacgttgttg
     1921 tcatttttgt tcggactcag gcgactgacc acttccgggc tgacgcgaaa cgttctggcg
     1981 agtcgttttt tggagtgttc gcgggctttt tcctccgtca tctgaatgat cagcatatcc
     2041 gcaggatcgc aaatcacgtt gtaaatcacc cagccgtcaa tcaggccgat agtcttgccg
     2101 gttcgtgccg ggccaacaaa tatcactgcg tcgtattcac gcgaggccag gcagttcatc
     2161 ggctcaataa catacggtgc caccagcgga tcccacggga ctgagttccc ggcccccatg
     2221 ggcacccgca tatactgagc aacggcatca gcaacccgca ttcgtctcgg tgcgcgaagg
     2281 atataacctg aatcggttcg tgctgccttt gcggtttcct gattcagcat tactcctcct
     2341 gctgtaattc ctcctcatca tccgcacctg cttcggtcac ccgcagggct atctgatcgc
     2401 gcagatcatc aataatggac tgaacacggc tcacagcggc aggctgcaga ccacagtcac
     2461 gttccagaat atccggtaat gtctccagca cctgcacgac cgcttttgcc cagatggcaa
     2521 actcccgtct gacatcactg gccggaatga gttgtgccgt ttcctgttcg aacttaagac
     2581 gctcacgttc agactgatac caggctttgc gttcatgtgg atccatttcg ccctcagcaa
     2641 ccggcggtgg taaccccata aattcagtca gaatatcggt caaccggtat agcttgagtt
     2701 tgtcatgtcc accagcggga cgaatgtttt tcagtcttgc cacgacagtc tggcggtgca
     2761 gaccagataa agccgccagt tgattaatat tcagcaccag gtttttcaac tcatgatcca
     2821 tatttcctcc ggagagcttt aaacatgcat cgtgcgaaca actttaagaa aacgcgttcg
     2881 atgtcgaaca aaaaacactc aattcgacat acaaaaaaca aataaccatt aataatcaat
     2941 aagatgcaaa gatgatggtg gccgataaaa atgcaaaaac tagccttttt ccgcgacgct
     3001 cccgccccgt ggcaggccac cccaccggga ggagccgtca gcctgaaagc catgacgaac
     3061 gtctgataca gcccttgcat gaatggcatc gggataatcc agaaaggaat agcatcgacc
     3121 cacaagaatc tgtgtgagtg tcctgtttct tccacccccg cacaggactg gcgagcatga
     3181 gggacaaacc cgcgaatcat tagcgcggta aaaacccggt gtgcatcgtt tttgattatt
     3241 cccgcacact cgcgcagaag gagttccccg tcgggctacg gtcatggtta atgcgggaat
     3301 acagcgacga tacagcgcat gatgtgtcag gcttgaatac ctttatccgt taaaagggat
     3361 atcagttaag ttatcccgtg tagggtataa gccattatca aagccactct gtagggagtg
     3421 gcttttgtaa tggcaataaa aaagccccgc gaatgcgagg ctaaatccag gtatttttaa
     3481 tgactggctc ttatctcaac gcagcccctt accgcgcgcc agatgctcaa tatcaagcat
     3541 cagcaatgag atgtttaacc atgattcact ccagaagtga gcaccaccct gtctacagag
     3601 ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg cgtcctgatg
     3661 tattcctgaa gcgttctcag tgctgtttgg tcgcggataa ttccgtcccg gataccgaga
     3721 acgtttcgtc cagcaactgg agagagttcg acggtggcat cattgcccat gccggaggcg
     3781 ctggaggttt cgtctgagga tggcacaggg cattttcctt tgacgaacac ccgaccacca
     3841 ttatcaagct tgcgccgaag agcatcattt ttagctttcg catcagctaa ctccttcgtg
     3901 tatttagcat cgagtgcatc agcagcacgc tggcgctgct gcatgtcagt aatggtggcg
     3961 gtcgcctgct tcagctcact gactttttta tcacgctgtt ctttataggc gatggcgtta
     4021 tcacggtaat gattaaccgc ccatgactgg cagacgatga tgcagataac cagagcggag
     4081 ataatcgcgg ttactctgct cattgttgcc cccacaaaca gacttcacgc tcaatctcgc
     4141 ggcgagtcat cagccctttc cattgcttac cgccagcgta tgtccagcgc cgtagctgat
     4201 cacatgcgcc tttgatatca ccctggttta ttttgcgaag aagcgtcgat gttctgaaat
     4261 taccagcgcc cacgttgtaa acgaacgagt aaagagcgcc gcgcgttgtt tccggtatat
     4321 cgactttgat gtacgggtta atttgtctgg cgaccgtggc aaggtcttta ttcaggaggg
     4381 ctttgcattc tgcttcggta tacgttttac cgggaatgat gtcttttccg gtgtgtccgt
     4441 gacatacagt ccatacgcca acgatatctt cgtatggtat gtagctgaca ccttccaggc
     4501 catcgtcacc actcggacca gtgatgagca cagacgctat ggcagcagcc ccaccaccaa
     4561 tagcagcagc aacagccttg cgtaatgatg gcgacattat tcacctctcg cagccttacg
     4621 cttatcttct ttaatcttga aataaaggtt tgtcagatac gtcagcaagc caaacaccaa
     4681 gctacccaat acgcctattg ccacccactg ggatggggag actttgtcca gcaactgcag
     4741 tagccagtat cccgtcccca ccgctgacgt ggtgtatgac acacctgttg ttattttttc
     4801 catctggtac ataccccgtc tcccgttatc cggaagctga caacaataaa aagccaccag
     4861 ttaattcctg atggccctga tgcataaacg tcataatacc tgactgttat gattgacaat
     4921 aatgataatg tttatataga aaggttcccg atgtgtgtta catatcattt ctccacgggg
     4981 aatatcccca cgccagcgca gactctttta cccgttctct tctgcgctgg ctctttttta
     5041 ttatgctgct gcatttacct ctggcaccat gctttctatc tcaacacaat acgtggtact
     5101 tcttgtaacc aatatcataa cgattaatcg acatagaatt tctcccgtgt acaggaacag
     5161 agttaaaaag ccggaaccgg aatcaaatca caggatgacc atctgccagt ggcaggtcat
     5221 aaaaaaaagg ccgcgccatg cgcagccaga actcacaagg aaaatgatag aaggaaataa
     5281 cattagtgat gtacgcatgg cgcctcccgc taagttctgc aatgatcaaa cagaactcgc
     5341 tacgtgccct taaaactcga tcatttagcc cctccaagga ggattcacca tgcggttgat
     5401 tttttaataa acagtaaaca aaaaagtcaa gaattattca ttctgttctt tcatcatcgg
     5461 ccacagcaat accacaatgc cgcagaccag agcgccatca gtcagtacca acattatcct
     5521 gctggtgaaa tccatcatca ccatcactaa aagcaggatc acaacagcaa gcagacacag
     5581 tttataaaac aatgttcagg aaacgcattc agcatgccta aggttctatt cctacgaata
     5641 gccaacttgc aacttaaaat attatttatg cagccaatta aattctggtc cttacaatat
     5701 caacctgaag attcttatct tgtgctgatt gataaatgac aaacctttta ctacctgcat
     5761 tgaaagaagt agacaaaacc agacaattat cataacgagc aagaacataa taccaaccat
     5821 cattataatt aatcatttca tattctttct taaactgtgg tttgtaatat cctgtcagaa
     5881 atgaaaaaag ccagaaatat gccacaaaag caatcatcac aatctcaaaa aaatgttttt
     5941 ttataaatgg cttatcatag aagcatgata ccgataaaaa tcgcccataa gatcttatcg
     6001 aaattgtaac cgccagcgca atcgctgctg acagtagcaa aagaggtacc tgaatcttct
     6061 gtctcaatat agaaaactca ataattgccg gcacaaacaa taattccaca gcaaaataaa
     6121 ggcgaaatac atttagctct tgcatagaat gttttctttt cactgcgaaa aagaatacaa
     6181 caccaatacc ccaaccgata agaaatatag caatgacgat aactgcaaaa aataaacttc
     6241 tggcaacatc atcaacacct gcacctacaa tccaccatgg gaagccgtag taaaaagaag
     6301 taccccatcc atagaaataa gcactccccc atccaaggca tcccatgtag gcaataaaaa
     6361 gtgaagaact cctgagcagc gcaccatcct tcataaccac cccaatacaa gatgataaca
     6421 ttggcttaca actcataaca aaagcaattc aatgccgtca agaggttaca ggctaaaaaa
     6481 actctattac atagcagcca gcatgtttac cgtacaagta caactcaggg cataaaaaaa
     6541 cccactcggc agcgggttta tacatttttt acaacatacc aaatttgcat gaagtatatg
     6601 gcttttaatc cagttttgca atattttgct gtaaaaatgc tgccttttgt tttgaacgtg
     6661 ttctcgtcac aagcaataaa gcatcactat caagctgtag aaaaatgtgc ttcattgcaa
     6721 cccagcgttc agtaaatgtc tcggaccagt tttttgttgt cactcccacc aatgatgcca
     6781 gtgtctggta ttcataggcc tcacgccctg caagttcgct cttcacatcc tgtgcagcca
     6841 gccagattaa cgtcttcagg cgatccagtg tcttacctgc aatttttctg ttacttaaca
     6901 aatctttaaa ctcgctccat gcccattgcg ttatggtgac ctgatgcccc catcgaacgc
     6961 tttcgctgta acaccaaagc aaccatgctt tctgatgttc atcgagagac aaaaccgcgc
     7021 ggcgccatga agaggttgag aattcaaccg ggctgaccaa agcaatggat gaaccttttg
     7081 cgtacgactg cttaccggaa gtcggcgtat tatccagcgt aatcatcttg ccagttacca
     7141 catccagaat gcgcggcttc tttcgtttgt atgtaccagt atcaaattgt gcatgctcct
     7201 gccaggcttc gagctggcct ttcgttgctc cgttcaagtc agcagtagct gccataagtt
     7261 gctcacgaac atactgtaaa tattgggtat tcatgcagta aatcctttct atattttggc
     7321 ataattcttc aacattcggt aatcgttcaa aaccgaatcg gggaaacgac ataagcacag
     7381 gagcccccag cgacagcgaa ggagttctga tatataagac tcagacatca ttcattcccc
     7441 ggttctccaa tatctgtttc actcatcatc cataactacc tgtaattgcc cccccttttt
     7501 ttgta
//
LOCUS       DAZGXS010000181         7442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGXS010000181 DAZGXS010000000
VERSION     DAZGXS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..681)
                     /locus_tag="ACHKG5_004350"
     CDS             complement(<1..681)
                     /locus_tag="ACHKG5_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0870329.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
     gene            complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHKG5_004351"
     CDS             complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHKG5_004351"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0870330.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHKG5_004352"
     CDS             complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHKG5_004352"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0870331.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHKG5_004353"
     CDS             complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHKG5_004353"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0870332.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHKG5_004354"
                     /gene_synonym="priS"
     CDS             complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHKG5_004354"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0870333.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHKG5_004355"
     CDS             complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHKG5_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0870334.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1790 a         2014 c         1965 g         1673 t
ORIGIN      
        1 accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcacct ctggtccggc
       61 ggcttcgaag atgcggtgtt cgttggctgg atggtctaac agcgccacca gatagtgcag
      121 caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc cagcgtggcg gcgttaacac
      181 tggcaggttg tagaccatat cgcgcatgac ttcgaacgct gctgaacctg caccaacgat
      241 tattccggca cgcaattcgg tcactggtac attcgcttca cgaagaatgt ccgccgtagc
      301 ctgacgcgcg cgcagatggt ccgactgctc atgtggcggg gcctgcaacg aactgagaaa
      361 gattaattgc ttaactggta cttcacgtag cgcatcgcgg aagttgagag ccacctggcg
      421 ctcctgagcg ataaaatcgc cgccttcgcc catgctatgc accagaaaat agaccgtatc
      481 gatatcctgt aacagggccg gaaggttatc cggccagctg agatcgactt tatggcaact
      541 gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg ccaggatctg
      601 atgcccttgc tggctgagtg tgcgcaccag atgctgacca atgtagccac tggcaccaag
      661 aactaaaatg cgttgcggca cgtctccctc cttaacgcgc caggaatgcg cgccagtggg
      721 cggcgacttc tgccagttgt tcgcgcgaga cgtcaagatg ggtcaccagg cggacaatcg
      781 gcgaggcgtt aatcagtacg tttctcgctt tcatgtattc gcctaacgcg gcagcatttt
      841 cttcgccgac gcgaacaaac agcatattag tgtcctgacg catcacatcc gcgcctgctt
      901 cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc ttcctgcaac cgtgcgacgt
      961 tatttttcag cgcatacatc ccggcggcag ccagaatgcc ggactggcgc atcccgccac
     1021 ctgtcatttt ccgccagcga atggcacgtt taatgtaatc acgattaccg acgagtaatg
     1081 aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca caatattgcg
     1141 tgatctcttt cagttcgctg ccgtaagcca ccacggcatt aaagatgcgc gcaccgtcaa
     1201 catgcagcgc cagattgcgc tcgcgggtaa attcccatgc atctttaagg tattcacgcg
     1261 gcaacacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg gcgaaatgga
     1321 tatcgtcggg tttgattttc atcgccactt tatccagcgg tagcgtgccg tcggcagccg
     1381 cgtcgatggg ttgcggttga atactgccca gcaccgccgc gccaccggct tcaaacagat
     1441 agttatgcgc ggcctgaccg acaatatact cttcgccgcg ttcgcagtga ctgagcagag
     1501 cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc ggcttcttta ccggaaagct
     1561 ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg tcgtccccaa
     1621 ccggggcggc catcatcgct tcgagcatgg cgcggctcgg tcgggttacg gtatcactgc
     1681 gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt taccttagcc
     1741 agttcgtttt cgccagttcg atcacttcat caccgcgtcc gctgatgatt gcgcgcagca
     1801 tatacaggct gaaacctttg gcctgttcga gtttgatctg cggtggaatg gctaactctt
     1861 ctttggcgac caccacatcc accaataccg gaccgtcgat ggagaaggcg cgttgcaggg
     1921 cttcatcgat ttcagaggct ttttctacac gaatacccgt aattccgcac gcttcggcaa
     1981 tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt caggtagcct ccggctttca
     2041 tctccatcgc cacaaagccc agcacgctgt tattgaagac gataattttc accggcagtt
     2101 tcatctgcac taccgagagg aaatcgccca tcaacatgct aaaaccgcca tcgccgcaca
     2161 tggcgaccac ctgacgttcc ggctcggttg cctgcgcgcc cagcgcctgc ggcatggcgt
     2221 tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc attttcagat
     2281 aacgcgccgc ccacaccgtt ggcgtgccga cgtcacaggt aaagatggcg tcatcggcgg
     2341 caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg ctcggtttag
     2401 ctaaatcgtc cagccctttg cgggcatcgc ggtaatcttc cagcgcttta tccagaaact
     2461 tgcgatcggc tttttcttcc accaatggca gcaatgcacg cagggtcgac ttgatatcgc
     2521 cgaccagcgc catatccacc ttgctgtgtg cgccgatgct ggctgggttg atatcaatct
     2581 gaataatttt ggcatcggtc gggtagaacg cacggtaggg gaactgagta ccgagtagca
     2641 ctaacgtgtc ggcgttcatc atggtatgga aacctgacga gaatccgatt aacccggtca
     2701 ttccaacatc atacggatta tcgtattcga catgctcttt accgcgtagg gcatgaacaa
     2761 taggtgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc gcgcagccgc
     2821 tgccacacat cagagcgata ttgctggaat aacgcagcag ttgcgccagt ttgcgtaact
     2881 cttcttcttc cggcgtcacg actggctgtg gcgcatgata ccagtgggta gttgcccctt
     2941 ctggcgcagg ttttaacgcc acgtcgcccg gtaacacgac aacggaaacg ccacggttaa
     3001 gcaccgcttt gcgcatggca atcgccagca cttgtgggat ctgctccggg ctggaaacca
     3061 gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc tggaaatagc
     3121 cgctgccaat ttcgctggaa ggaatatgag cggcaatcgc cagtaccgga acgtgattgc
     3181 ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc ggggccgcac gatccagcac
     3241 agaccgccag ttctcctgaa agttgtgctt cagcgccagc ggcaaacgcc gccacttctt
     3301 catggcgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtcg ctaagaccgt
     3361 tcagagagtc gcccgtgact ccccagatgc gtttcacgcc tgccgattcg agtgttttgg
     3421 cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg ataacggtaa
     3481 caagtttagt tcatctgacg gagggggaag ggatgggaga gaaaggaggc actaacggtt
     3541 aaatagcccg atgaaaggaa tatcatcggg cataaggcga ttatgcgaga accaaatccc
     3601 cctgcggatg gcaggagcag gccagtacgt aaccttcagc gatttcggcg tcggtcagcg
     3661 tcattgtact gctcaccgta tattcaccgg aaaccacttt cgtcttacag cagccgcaaa
     3721 cacccgcgcg gcaggcggcg acaaccggaa cgttattgct ttccagcgcc tccagcagcg
     3781 tggtgccaac aggagcgtaa aattcttgcg ccggttgcag tttggtgaat ttcagaccgc
     3841 tggtcgccgc ttccgctact ggggtgaaga atttctcttt aaagaaacgc gtcacgccga
     3901 gtgctttcac ttcctgctct acccaatcca tatacggagc cgggccgcag gtcatcacgg
     3961 tacgtgaagc taagtcagga acgcgagtca ggagttcgcg agtgagacga ccagcgataa
     4021 agccttcggt aacgttattt tccgccacca gcgttaccgg atagttacgc cactcatcgg
     4081 cgaaaataac atcctgcggc gtacgcacgt tgtagatcac ccgcacatcg gcctgtggac
     4141 ggttcttcgc cagccagcga cgcatcgaca taatcggcgt gacgccgcag cctgcagcca
     4201 gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa ttcccccatc gcgtctgaaa
     4261 gccagagata atcaccgcgt tttacatcgc gggtcagcca ctgggagccg acaccgtcat
     4321 caatccgccg cacggtcagg gtgatatatt cactcacgcc tggcgtggag gaaatggtgt
     4381 aagcacgcag cgtttccgct gagttacgca cgctgaccag tgcatattgc ccggcgcggt
     4441 atgggtagta atcgtggcaa atcagggaaa tcgtccacac atccggcgtt tcttgcttaa
     4501 tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg catcgtcatc gacaaactcc
     4561 ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac gcaggccgaa
     4621 tttctcgttc agcaccgcca gcagatctgg tgtcaggaaa ccaggtgcag tcgggccggt
     4681 gacgatattt ttcacgccca gagaaagcag cgtcagcaga atgacaatcg ctttctgttc
     4741 aaaccaggag agcaccagcg acagcggcag atcgttgaca ccgcagccca gtttttctgc
     4801 cagcgtaacc gccagaataa ttgctgagta agcatcgtta cactgaccgg catccaccag
     4861 acgaggcaga ccttcgatat cgccaaactc aagtttgtta aagcgatatt taccacaggc
     4921 gagagtcagg atcaggcagt catctggcac gctggtggcg aaatcggtga agtagtgacg
     4981 ttcgccgcgt gcgccgtcac agccaccaag caggaagata tgacgcagtt tttcacggct
     5041 caccagatca attagcgtat ccgctgcgcc aagcagcgtc tggcggccaa agcccacggt
     5101 gatcaggtgc ggaatttcgc tgtatgggaa gcctgccatt tgttgcgcct gggcaatgac
     5161 cgcagagaaa tcttcgccat ccagatgacg cacgccaggc cagccaacga tgctgcgggt
     5221 ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg aggtcatcac
     5281 gatggggcca gggaaacgag cgaactccac ttgctgattc tgccagccgc tgccgtagtt
     5341 accgaccaga tgcttgaatt tacgcagctc cgggtagcca tgcgcaggca gcatttcgcc
     5401 gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc agcaggttgt agagatcttt
     5461 gagatcgtga ccggaaatca ggatgcattt acccgccgtc gctttgacgt tgacctgggt
     5521 tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc agaatgctca tcactttgaa
     5581 gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg caggccaggt
     5641 ccccagccac gccatgattt tatgatactg ggcataaata tcgttgtcgt attgaccgag
     5701 aacgtgtgcg tgttccatat aggccgccgc acctttcagg ccatacaggc acagcagacg
     5761 caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa attctgctgc
     5821 ctgacgttgc agttcgccga gatcgtcgct caccagttgc aggtcagcca tcgggttatc
     5881 gacgcgggcg ttggcatcta cagccaggca ttgtgctttc agcgcctcgc gcaaggcaat
     5941 cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg tcagggttga
     6001 gaaaaaggca cgtggcgcga agctgtcgac atcgtggttg atgatgccgt attcacgcgc
     6061 ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg agtaaatcct gaaggtcaga
     6121 agtttccgcc gttttaccac acatcccctg cgcgtatgag cagccgtttc ctgccggagt
     6181 acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag ttatatttaa
     6241 tatacatgtt taaggttaag acgcttaacg cggggataaa agggattttt catgcaactt
     6301 taagggagat tgatttagcg caattttggc ggcagggatc taccgccaga gaggtattac
     6361 gcggagaaaa aggcgatgag gatcggcact aacaggctaa gaataaaacc gtgaacaatt
     6421 gccgccggga ccatatccag cccgccagta cgttgaagaa cgggcagggt gaaatccatc
     6481 gacgtcgcac cgcataagcc cagtgcggta gagcggctgc ggcgaatcag cccagggatc
     6541 aacataattg caatcagctc acgggccaga tcattaaaaa acgccgcgct cccgattacc
     6601 ggaccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa accagaggcc
     6661 attgccagcg cggtattgat gggtagatca agaataaagg cgttaattaa accaccaatt
     6721 aatgaactgg caaccaccac cacggcgaca atcattcccc ggcgattaag gacaatctgc
     6781 tttaaggtca tgccattatt gcgcaactga ataccgacga ggaaaagtag cagaattaac
     6841 gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca gtccactcag gccaatggca
     6901 aaaccaatca ctactacgcc gcacagtttt agcgactcaa gcgccatcgc aatacgcgac
     6961 gggagttttt cttgctgatg gtggttgcgc cacggcagac cgcgctccag ccacataagg
     7021 gcggcaatat tacacagtaa aataacggta atactgacgg cagaataatg cagaatcgcc
     7081 aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa aaagagaata
     7141 aggtaaacca tccagcttaa tagctgatta ataactttta acgcagcttg ttggcgaagc
     7201 ggaatgaggt aacccacaat caggggaacc agaatgatta acaacccaga aaacatgaaa
     7261 aaccagccct tgcaaagaaa gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
     7321 gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca gagggggatt tttaattcta
     7381 tatgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa gccactggcg
     7441 ca
//
LOCUS       DAZGXS010000182         7355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGXS010000182 DAZGXS010000000
VERSION     DAZGXS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..467)
                     /locus_tag="ACHKG5_004356"
                     /pseudo
     CDS             complement(1..467)
                     /locus_tag="ACHKG5_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1172..1306
                     /locus_tag="ACHKG5_004357"
     CDS             1172..1306
                     /locus_tag="ACHKG5_004357"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870335.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1908..2729
                     /locus_tag="ACHKG5_004358"
     CDS             1908..2729
                     /locus_tag="ACHKG5_004358"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0870336.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2729..3835
                     /locus_tag="ACHKG5_004359"
     CDS             2729..3835
                     /locus_tag="ACHKG5_004359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0870337.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            3925..5646
                     /gene="cptA"
                     /locus_tag="ACHKG5_004360"
     CDS             3925..5646
                     /gene="cptA"
                     /locus_tag="ACHKG5_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0870338.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5720..6718
                     /gene="lpxM"
                     /locus_tag="ACHKG5_004361"
                     /gene_synonym="msbB"
     CDS             5720..6718
                     /gene="lpxM"
                     /locus_tag="ACHKG5_004361"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0870339.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            6771..6959
                     /locus_tag="ACHKG5_004362"
                     /pseudo
     CDS             6771..6959
                     /locus_tag="ACHKG5_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7063..7149)
                     /locus_tag="ACHKG5_004363"
                     /pseudo
     CDS             complement(7063..7149)
                     /locus_tag="ACHKG5_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1924 a         1577 c         1796 g         2058 t
ORIGIN      
        1 accgatggta aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta
       61 ggccagaacg ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt
      121 actgccgata aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc
      181 gaccggggat gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta
      241 atgccgattt tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca
      301 gtgatttggt cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact
      361 cgatgacagt cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa
      421 acctgaacgg actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa
      481 aagtgactat tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc
      541 gccttgtgga ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc
      601 aataaataaa tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag
      661 agattgatac gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta
      721 ataccgctac actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga
      781 ctggtgtatg gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt
      841 cggagaaaag gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt
      901 ttcattttct tctccattat ttataaggga ggtgtattat tatgcaatat tattggtttt
      961 atccattgtt tttagttcat gaatgtttgc aaaaaaataa atgaacaata attttatgta
     1021 tttaaaataa attgatttta cttctgtaat ggtgtcatga ttagcagctt tttaaactaa
     1081 tatcgacttt caataaataa ggacaggtaa tggacaacaa aaatatctga acccgggtga
     1141 gattcataag ttgcacagtg ctgtcttcaa agtgccacac ccggaacgta atcactgcct
     1201 gctccttatg gggtatcttc atggtgtcca agcatcagag ttattaggta taaaactgtc
     1261 tgatattgat ttgcaggccg gtaacctgaa tatccggcgg ctctaaacgg tttgtcaact
     1321 cagcccctcg gctgaaacag gaggtacagt ccattcgtcg ctggctgaaa cttcgggatg
     1381 aatgggtaga agagggatat gaatggttat ttaacctcta tggcaggtct gctacaggta
     1441 ttactgttgt tctggggggc actggtgtgg ttaatatcta cgatgcaaga gattctcacg
     1501 atggcccggt ccatcagtgc atgcggcgcg atattgtgcg agtatttttc agggggagtg
     1561 gtatccggat gcagaaaatt ccgctactcg actggtttta tgcaataaat caggatgata
     1621 atcttctgat tttttactga ggcggatatc tctcgctgtg tttactgaaa ttgcggtatt
     1681 ctgttttttc tgaaaaacag aaaatctgat taaaaagagc atatgcgtta agggaaattt
     1741 aagttctggt tgattaactg ataaaaatat taaaaaatcc ctgttgtcgg taatctccgg
     1801 accattccgg gattacacaa taatatcacc atgtgtgata tctatggatc ctcactgttg
     1861 tttacgcgag gatattttca gaatgcttaa cagacgatta tttttttatg ttgaatgcaa
     1921 ggcatcttcc cgtactgatg tatcatcatg tcagtcgttg tccggggctt gtgacactct
     1981 cacctgaaac attttgtgaa cagatgaagt ggctcgcaga taataactgg cggacggtaa
     2041 catctgctga gcttgaatat ttttatcagg ggggaacgct tccacgaaag agtgtcatgc
     2101 tcacattcga tgatggttat cttgataact ggctcagagc atggccggtc ctgaaggaat
     2161 ataaccttca tgcacatatt tttctcatta cggggctgat tggtaaggga aacgtacgta
     2221 gcaggcagga gcaggaatat tctcaccgcg actgtgaacg gttgattgcc gataaccgtt
     2281 cagatgaggt catgcttcgc tggtctgaag tccgggaaat gcgtgattcc ggactggttg
     2341 aatttcatgt gcacacccat tcacataaac gctgggacag gttgtctgta tcacgtgctg
     2401 aacagtgccg gctcatgaaa gaagatattc tggtgggaaa acaatgtctg acggaaaagc
     2461 tggggttctg tagttcccat ctgtgctggc ctgaaggata ttataatcgg gattatatta
     2521 atctcgccgg taagcttggt ttctcttatt tatatacaac cgaaagaaga atgaactgtc
     2581 ctgaaaatgg ttcattacgc atagggcgta tcagcaccaa agagcgggaa cattccggct
     2641 ggctgaaacg acgccttttt tattatacaa cgccactttt ttcttcagta ctggctcttc
     2701 ataaggggcc aagattacca gataattaat gatgaaaatt ttattcactg aatcttcgtc
     2761 agatattggg ggccaggagc tgcaggcact ggcccagatg actgctcttc aaaagcaggg
     2821 acattccgtt ttacttgcct gcagagaaaa aagtaaaatt gctccggaag ccagaaaaag
     2881 agggcatgat gtcactttta ttcctttcag gaacagcctt caccttcctt ccatcctcag
     2941 acttcgacgg attatcgggg aatttaaacc tgatctggtc atctgccata gtggccatga
     3001 cagtaatata gcaggactga gccgtctgat atgctgtcac cgcttcagta ttgtcagaca
     3061 aaaaacctac atcacccgta agaccagaac gttttccctg aattatctgt gtgatttcat
     3121 tgttgtgcct agctcagcca tgatggcgca tctgatggcg gaaggtgtca ggacaccggt
     3181 aacggttatt ccaccgggtt tcgactggcc cgcattgcat aatgaagcca tgcgaccgtt
     3241 acccctgcat atccacgcct gggctgcatc agcagacaat gttccgctta tcgttcaggt
     3301 ggggatgcta cgtcccgaaa aaggacatga atttatgctg cgtgtattgt atcagctgaa
     3361 gatggaaggg aaatcatttc gctggctcgt ggtgggcgcc gggcgtgaag aatacgaagc
     3421 acgtctgcgt cagcagacag aacacctggg aatgagcgga gatgtgttaa tggcaggtgc
     3481 ccttttccca gcgttaccgg tctaccggat tgccagtgtg gtggtcatgc cttcggaaaa
     3541 tgaagcgttt ggtatggtac tggctgaagc gtcagtctcc ggcgtgcccg ttatcgccag
     3601 tgagacgggc gggatccctg atgtcattca gaagaatgtg acagggacgc tgttacccgt
     3661 gggggatgtg tctgcatgga ccggcgcact gcgcgatttt ttatcccgac cggaacgctt
     3721 ccggatgatg gcagccagcg ccagagaaga tattgagtac cggtttgata ttaacagaac
     3781 ggcacagatc attgtgtctc tggctagtca ggctaagggg aaatgcaaca gataacggcg
     3841 gatcctcttt tcagcccgaa ttttgttcag cggttttccg gtaacggagt aatttatcac
     3901 attgtttaaa tacggggacg gtcaatgcat ttaaataccg gacagaacag gcctactttc
     3961 agctggagcg cgctgggatg ggcaattttt tattttggct ttttttccac tctcctgcag
     4021 gtcatcatct tcagcagtgg gtacagcgga acgaatggaa tacgggactc actgttattc
     4081 agttgtctgt ggttgatccc ggtgtttctc tatcctgatc ggataaaaat aattgcagct
     4141 gttgtcggtt tcattctctg gggcacgtcg ctggcagcac tttgttatta ttttctctat
     4201 ggtcatgaat tctctcaaag tgttcttttc gttatgtttg aaacgaatgc cagagaggct
     4261 ggtgaatatt tcagccagta tttcagcctt aaactgttgc ttatctcgct ggtatatact
     4321 gcggtgtccg tttttctgtg gacacgtctg cgtcctgtat atattccttt gccatggcgg
     4381 agaattgtct ctttcctgct gctttatgct ctgcttctgc atccggttgt tctgaaatcg
     4441 ttaatcagac aggagccgct gaatgatact ctaggcaaac tggcatcccg aatggagcct
     4501 gctgccccct ggcagtttgt atccagttat tatcagtacc atcagcaact gaatgcactg
     4561 acaaccttcc tgaatgaaaa tagcgcactg ccaccactgg gtaatctcag ggatgaatca
     4621 ggggagagac cacgcacact ggtcctggtg attggtgagt cgacacagcg cgaacgcatg
     4681 agcttatacg ggtatctacg tgaaacgacg ccggagctgg atgcactgcg taaaaccgat
     4741 ccgggtctta ctgtgtttaa taatgtggtg gcatcgcgtc cgtacaccat tgaagcattg
     4801 caacaggccc ttactttcgc caacgaaaag aatcctgatc tgtatctgac gcagccgtcg
     4861 ctgatgaaca tgatgaagca ggcaggctat aaaacattct ggattaccaa ccagcagaca
     4921 atcacagccc gtaacaccat gctcactgta ttttcgcgcc agacggacag gcagtactac
     4981 atgaatcagc agcgaacaca aagtgcgcgt gaatatgaca ctaacgtgct gaagccgttc
     5041 cgggaagtgc tgaatgaccc tgcaccaaag aaactgatca tcgtgcatct gctgggtacg
     5101 cacattaagt ataaataccg ttatccggaa ggtcaggggc ggtttgatgg cattacaggg
     5161 catattccca ctggattaaa tgcgaaagag ctggaagtgt ataacgatta cgataatgcc
     5221 aatctgttta acgatcatgt ggtggccagt ctgataaagg acttcagagc aactgcgccg
     5281 gacggctttc tgctttattt ttcagaccat ggtgaagaag tatatgatac tccgccatat
     5341 aaaacgcagg gacggaatga agataacccc acacgtccca tgtacactgt tccgttcctg
     5401 ctgtggacct cggaaaagtg gcatgctgca catccgcgag atttttcgca gtatgttgac
     5461 cgcaaataca gtctggctga actgatccac acctggtcag atttggcggg actgacatac
     5521 gatggttacg atccgacccg ttctctggtg aatccgcaat tcagggaaac cacccgctgg
     5581 attggaaatc cgtataaaaa gaatgggctc actgatttcg acactcttcc gtatggtgag
     5641 ccatagtatt ttcttatctt cggttccgtt ctgaaggcag gaggatgtgt tttcctctgc
     5701 tgtccggtgg cctccaggga tggtaccacc ttctgctgta ctctgttatc ataatgagat
     5761 aagcaggcag ataccggtaa atatgaaaaa tatcagaact gagtttattc cccgctttaa
     5821 ccttacatta tgcttccccc gttactggat gacgtggaca ggtatcggta ttatctgtgt
     5881 gtttgcaatg gtcccccccg cactgagaga tccattgttg ggaaaattgg gcatgctggt
     5941 tgggcgtctg gggaaaagcg cacgtcaacg ggccttgatt aatttatcgc tttgttttcc
     6001 ggagtacagt gataaagaaa aggagaatat cgttgatgcc atgtttgcaa cagcctccat
     6061 ggccgttgtg cttatggctg aattagcact gtccgggcct gataaaattt cgcatcgtat
     6121 acgctggaat ggtctggaga ttgtggagaa gatggcacag aacaatgaaa aggtcatttt
     6181 tcttgtgcca catgcctggg gagtggatat tcccgcaatg ctgatggctg cgtcgggcag
     6241 aaaaatggca gcgatgtttc ataaccagag aaatccggtg gtggattatg tctggaacag
     6301 tgttcgtcgt cgtttcggag gaaaactgca cgcacgtaat gacggtattg cctcctttgt
     6361 ccgctcagtc cggcagggat actggggata ttatcttcct gatcaggatc atggcccgga
     6421 attcagtgag tttgcagatt tttttgcgac gtataaagca actttaccgg ttatcggacg
     6481 gctcagcagg atcagtggtg cacggataat accgcttttc cctgtttatg acggaaagac
     6541 ccatcatctg actattcatg tcagcccgcc acttgctata cgacagaaga gtgatgctca
     6601 tattgcgcga cagataaacg aagtggttga aaatttcgtt cgtccacatc ctgagcagta
     6661 tacgtggatc ctcaagttgc tgaaaacgcg taaagaaggg gaagaggatc cttactgagg
     6721 cagctttttc aggataatga gcttacccag caatagtgga cacacgctaa gtgagtaaac
     6781 tctcagtcag aggtggctca tatgacaaaa aaacagtacc aaccagtaaa aaaccccgta
     6841 aatagcattc gcctgaattt cgcagtgaag ccctgaagct tgctgaacgc atcggtgttg
     6901 ctgtcgcagc tcgtgtgctc agtctgtatg aatcacagct tgtaagcggc tcgtcagaac
     6961 tgtattgata tttactgagc ggtcagttca acgtaagcga gtgttatgcg ccatggaagc
     7021 ttacccagga atatgttatc aggcctcagc atggcgaagt gttgcaacgc tggaagcaga
     7081 atgaccgcgg tcaacaactt gttttaccgc ttcaatttta aaatcttcgg gataacgctt
     7141 accgctcatg ggcacctctc tttaagccat ttaaaatgac tctgaggtgt ctgttaaacc
     7201 cgtggcgatt caatctggat atagtggcta agaatacctt tatttttttg gtttttattt
     7261 tttattgagt tttgttttgt atattaaata tttgttgttg attcattatc acacaaaaga
     7321 atgtatcata atgcaattgt ttgatgtgtt aaacg
//
LOCUS       DAZGXS010000183         7252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGXS010000183 DAZGXS010000000
VERSION     DAZGXS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..744
                     /locus_tag="ACHKG5_004364"
     CDS             <1..744
                     /locus_tag="ACHKG5_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0870340.1"
                     /translation="AGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDR
                     GGHGSVDSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLG
                     GHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSY
                     NMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSST
                     LSGHLGVGYSNGAGMESPWNAVAGVNWSF"
     gene            complement(1116..1382)
                     /gene="minE"
                     /locus_tag="ACHKG5_004365"
     CDS             complement(1116..1382)
                     /gene="minE"
                     /locus_tag="ACHKG5_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0870341.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1386..2198)
                     /gene="minD"
                     /locus_tag="ACHKG5_004366"
     CDS             complement(1386..2198)
                     /gene="minD"
                     /locus_tag="ACHKG5_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0870342.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2222..2917)
                     /gene="minC"
                     /locus_tag="ACHKG5_004367"
     CDS             complement(2222..2917)
                     /gene="minC"
                     /locus_tag="ACHKG5_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0870343.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3437..3805
                     /gene="ycgJ"
                     /locus_tag="ACHKG5_004368"
     CDS             3437..3805
                     /gene="ycgJ"
                     /locus_tag="ACHKG5_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0870344.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3908..4309)
                     /gene="pliG"
                     /locus_tag="ACHKG5_004369"
     CDS             complement(3908..4309)
                     /gene="pliG"
                     /locus_tag="ACHKG5_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0870345.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4380..4538
                     /locus_tag="ACHKG5_004370"
     CDS             4380..4538
                     /locus_tag="ACHKG5_004370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870346.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4550..4843
                     /locus_tag="ACHKG5_004371"
     CDS             4550..4843
                     /locus_tag="ACHKG5_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0870347.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4915..5574
                     /gene="ycgM"
                     /locus_tag="ACHKG5_004372"
     CDS             4915..5574
                     /gene="ycgM"
                     /locus_tag="ACHKG5_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0870348.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5666..6112
                     /gene="ycgN"
                     /locus_tag="ACHKG5_004373"
     CDS             5666..6112
                     /gene="ycgN"
                     /locus_tag="ACHKG5_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0870349.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6319..7230)
                     /gene="hlyE"
                     /locus_tag="ACHKG5_004374"
     CDS             complement(6319..7230)
                     /gene="hlyE"
                     /locus_tag="ACHKG5_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFP0870350.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1859 a         1602 c         1749 g         2042 t
ORIGIN      
        1 gcgggtgccg ggtttgagca gacgctgacc ggaatgacag tggggatcga cagccgtaat
       61 gatattcctg aggggattgc gacgctgggc gcttttatgg gttattccca ttcacatatc
      121 ggttttgatc gtggaggaca tggcagtgtg gacagttatt ctctgggcgg ctatgccagt
      181 tgggaacatg aaagtggttt ctatctggac ggtgtcgtga agctgaaccg ttttgaaagt
      241 aacgtagccg gtaaaatgag cagcggtgga gccgccaatg gcagttacca tagcaacggg
      301 ctgggcggtc acattgaaac cgggatgcga tttaccgatg gtaactggaa cctgacgccg
      361 tatgcctcgt taacggggtt caccgctgat aaccccgaat atcatttatc caatggcatg
      421 gaatcgaaat cagtcgatac ccgcagtata tatcgtgaac tgggtgcaac gctgagttac
      481 aacatgcgtc tggggaacgg tatggaagtt gagccgtggc tgaaggcggc tgtgcgcaaa
      541 gaatttgtcg atgataaccg ggtgaaagtg aatagtgacg gtaatttcgt caatgatttg
      601 tcgggcagac gtggaatata ccaggcaggt attaaagcct cattcagcag tacgttaagc
      661 gggcatcttg gggtggggta tagcaacggt gctggtatgg aatccccgtg gaacgcggtg
      721 gctggtgtga actggtcgtt ctgaccatca acgattaaac tgcgcttcgg cgcagttttc
      781 gtttacagga tgttaaaaga gaaaattctg gggtaaaaaa agcccgccag ttacggcggg
      841 aaacctcatc ctatgggaga acaatgaata atgaaattgc ggggttatca tctcccagta
      901 tatcaacact aacaataagg ttatttactc aaccaggcat aaatattttg ttttgtgcgt
      961 gggaacagcc ttaaggtgta aagggggagg tggaaatagc aatgaggagt atcagcaaga
     1021 atactcgccg ctttaccaca acgtggatga gagggatgaa aaactcaagg cagagataac
     1081 tctgccttga agataaatgc gcttttacag cgggattatt tcagctcttc tgcttccggt
     1141 aaggtcacgt tcagctcaag aatagaaata tcgccatctt tttgctcaag ctgtacggtt
     1201 accatctcag gatcaatttg tacgtattta caaatgacct caagaatatc tttacgcaac
     1261 tgcggcagat aatgcggttc tgcatcgctg cgacggcgtt cagcaacaat aatctgcagc
     1321 cgttcttttg caatgttggc tgtatttttc ttccgcgaga gaaagaaatc gagtaatgcc
     1381 ataacttatc ctccgaacaa gcgtttgagg aagcctttct tctcttcttc aatgaagcgg
     1441 aaaggacgtt cttctcccaa cagacgttct acggtatctg cgtaggcttt acccgcatcg
     1501 gcgttaatgt cgagaatgac cggttcaccc tggttagagg cgcgcaatac tgattgatcc
     1561 tctgggatca cgccgacgag tttgatgcgc aggatctcca gcacatcttc catgctcagc
     1621 atgtcacctc tgcttacgcg gcctgggtta tagcgcgtta acagcaggtg ctctttaata
     1681 ggctcttcgc cattttctgc gcggcgtgat tttgacgcca gaatgcctaa aatacggtca
     1741 gagtcgcgta ctgaggagac ttccgggttg gtggtaataa tggcttcgtc tgcaaaatag
     1801 agtgccatta acgcaccggt ttcaatccct gccggggagt cacaaacgat aaattcaaaa
     1861 tccatcgctt tcagatcatc aagaacttta gcgacccctt cacgggtgag ggcatcttta
     1921 tcgcgtgttt gcgatgccgg cagaatatag agattttcag tacgcttatc tttaattaac
     1981 gcctgattta gcgttgcatc gccctgaatg acgttgacga aatcgtaaac gacccggcgt
     2041 tcacaaccca taatcaggtc gagattacgc aggccgatat caaaatctat cacgacagtt
     2101 ttctttccct tctgggccaa accagtggcg atggccgcgc tggaggttgt cttaccaaca
     2161 ccccctttgc ccgaagtaac aacaataatg cgtgccatag aaattccttg ttaaaaaggg
     2221 atcaatttaa cggttgaacg gtcaaagcgt tttcgactaa ctgcagtcgc gccgctttgc
     2281 cataaaattc tgctgggatt tgatcactca gccagtattc acctgcgatg gacaccaatt
     2341 ccgccatcag gttcgtacaa aatatttgcg tttcccggtc accacttgcc cctgccagcg
     2401 cacgaccgcg catcatgcca tagacatgaa tgttcccatc ggcaatcaat tcggccccag
     2461 cgctaacgtg gcttgtaaca atcagatcac attgtggagc ataaatacgc tgaccggaac
     2521 gcaccggggt atctattaaa cgcgtttttg tgaccggcgt tgtattttgc gctggagcct
     2581 gcggtgcggg agctggacgt ggcgcttttt cctttccttc cgtcaggata ggcagcccca
     2641 ttttttcaat ttcggcttta agttgcgcat ctttgcagcc acttacgcca ataacccgca
     2701 aaccggttgc cgaaaccgcc ttatgcatcg ctgaccagtt taccgggtct tccagtgcac
     2761 tgacgttgag tacaacgggg gcatgtttta aaaatgcggg ggcctgagcg attttgtctt
     2821 ccagcgcctg atggataacc ttaggttctg cctcatgcag atgaaccaca gataaagtga
     2881 agctactgcc tttaagctcg attggcgtgt ttgacatcct ggccttactc aattagctat
     2941 taatcatcgc cagcgcgcga tgatgttccg aagactataa ggcatgttat agtctggatt
     3001 atattgaggc aagtcaccct cccatttatt cagagtaaaa gtctattctg tgataaatgg
     3061 cgctgattca tagcttaaaa aatacccttg tcaatcaacc cattgccgtc gtacttttga
     3121 ttcttcttat ttacgcttcc tttttccgca ccctaatcgt gacagcatct ccagacctgt
     3181 ttaaatatcc agtattgagg caagtgtatt ttttcaccac gccaggcttt cttaactttc
     3241 cattttttgc agcatcatta aagaacagat acccgttatc ttaatatccg ccacagaatt
     3301 gctctatcat agaaaccgca gaagacgtct gatacttctg tcaggtcatc gtaactcgtc
     3361 caggtcagcg gttttgatta cggattgatg atgaaatgag cataagtgtt gtacggttgg
     3421 attatcagga tgtgctatga atatgatgcg tattttttat atcggattgt caggtgtggg
     3481 gatgatgttc tcatcaatgg cgtctggaca tgacgctggc ggacttcaat ctccggcgtg
     3541 cggcgttgtt tgcgacccgt atatttgtgt gaactcagat ggcatttccc cagagttaac
     3601 aaggaaatat ctcagcgaaa aagccgctga aaacttacaa tcattacaag gctacgatcc
     3661 cagcgaattt acattcgcta acggtgtatt ttgcgatgtt aaagaaaaat tatgccgtga
     3721 tgatcgttat tttggtgtag atggtaagcg gagtggaaaa atcaatcaaa ccaccacaaa
     3781 gatgttattt atgtgtcgtg agtgatgtct tgtttacatt atgttctatt cgtcattaac
     3841 ctcttgagcg acagagagga gtggggcgtc aacgtttatg acggggagac tccccgtcgg
     3901 ttgacattta cttaatttga atatcgacgt tatatttttt agttttgttt ttacgggcgt
     3961 cgtttcgggt ttgcagcact cgcagctcat atttgccaga agcagggagt gagtattgtc
     4021 cgtgactatc gagttcaggg gaatatctgg ataaatcaac agaatcatcg attcctggcc
     4081 cgaagaggta agtatcagcg ccttcattag agatactcac atgcactttc tgaccttttt
     4141 tggcatagaa ggtatatgta tcgtaatcgt atccctttat ttcgccggag tactgagcgc
     4201 tgctatggcc ttttctgaac tcaacattga cattcttacc cgcagcaaag ctcgtggagg
     4261 ttagcagtgc aagcagcaat acagccttac tgatgctctt gattttcatt ggtagtcttc
     4321 tgttcagaat gagtacgatg aactgttata atataacaat cccttacggg agaattccag
     4381 tggctttcct gcgttcggta aaatcccgtc gcggcatttt tgccgggggg attacttata
     4441 aaggggcatg gactcaacaa accgtagaaa tttacagctg gctggcttgt ggcgcgggtt
     4501 tcattgatgt tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt
     4561 gatttatcga agcagcaagc gtgaccagac ctatttatat gtcgaaaaaa aagacgattt
     4621 ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct
     4681 gccgctggat gggcggaaga aactggtcaa tgccgatatt gaaaaggtta aacaggcatt
     4741 aaccgagcaa ggttactatt tgcagttacc gccaccaccc gaagatttgc tgaagcaaca
     4801 tctttccgtc atggggcaga aaacagacga cactaacaaa taaccgatat ccggcggtgg
     4861 cattatcttt gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat
     4921 caacatcaca actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtttgtgtt
     4981 ggcagtaact atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg
     5041 ctgtttatta aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc
     5101 gatttcggtt cagttcatca tgaagtcgaa ctagcggtgt tgattggcgc gacgctgcgt
     5161 caggctacgg aagagcatgt ccgcaaagcc attgccggtt acggcgtggc gctcgatctg
     5221 acgttgcgtg atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggctaaa
     5281 gcgtttgata actcttgtcc gctttccggg tttattcccg cggcggaatt caccggcgat
     5341 ccgcaaaata caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact
     5401 gcggacatga tccataaaat cgttccgctg atcgcttata tgagcaagtt ttttaccctc
     5461 aaggccggtg acgttgtgct gacaggcacg cctgatggcg tcggcccgtt gcaaagcggt
     5521 gatgagctga cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt
     5581 ttgccgcctg aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg
     5641 ttttaacgta atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct
     5701 ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct
     5761 gcataaactg atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca
     5821 gctcaatatt aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacccga
     5881 ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa tggctaccaa tgacctgcgc
     5941 ttatcgtttg ctggcggaag gtaaagattt acctgcgtgg catccgctgc ttactggttc
     6001 gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga
     6061 agtgattgac tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta
     6121 atagatgttg atgggggctt tcttaatata cgtctggatg ctcgccattt cgtagccctg
     6181 tttttgaaag cgctctgtca taatgagagt tacccgatac cttattatca acaggtaact
     6241 ctcaatcttt aaaaaacgtc tgtattttta aatgtgataa actttatacc ttgagtacat
     6301 ggagagaata attgctgatc agacttcagg tacctcaaag agtgtcttct taccgtgtct
     6361 tttctgatac tcattacagg tgttaatcat tttattggcc gcttctttta gcaaagaaag
     6421 cattaaatca tcataatcaa catagaatct ggttgtttca gtttccgttt ttatctcccc
     6481 gatggcggct atttcggtgg ttaatttcaa tttggcggca tcgatatctt tattcgcttg
     6541 tttaaccgtg ttagacaggg tggtaaagaa actctgcaca gattttaact tgttcttcaa
     6601 ttctggaatc agtttccctt caactacgcc cgcagcaata gaataggaaa tgattaaacc
     6661 aaatggaccg gcgacgacac cggctgcggc accggcatac gcttccttcc tgattttatc
     6721 tacctgtgac tggaaatagc tgcttttttc tgaaaaatca ttggttaact ggctatctaa
     6781 cgccagcagt ttcccggaag cgttgttgaa actttgtgag cttaccagca gggatttttg
     6841 cgcttcattc agcttcgtga tgccgtcatc cagtacctta atgagaatgt ctttctgggc
     6901 ggatgctttc ttctcattat actcatcaaa tagcaaaata tacgctgcga gcaattgcgt
     6961 cgcaacacca caccattcat acactgtttg ggttgcttca aaatacttat cctggctatc
     7021 cattagtaag gttttaatat tgccaactaa aacggaggct gcctgtgaat actcctgttt
     7081 aaagcgactt aactctttta tggtttcatc aaaggtctgc caggggatga cctgatcgag
     7141 atatttatta taaagatcca atgctccatc tgcggtttcg attgcgtttt taactacctc
     7201 taccgtttta tctgcaacga tttcagtcat aatcattcgc ctctttaaat at
//
LOCUS       DAZGXS010000184         7142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGXS010000184 DAZGXS010000000
VERSION     DAZGXS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(486..1268)
                     /locus_tag="ACHKG5_004375"
     CDS             complement(486..1268)
                     /locus_tag="ACHKG5_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0870351.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMLKQLS"
     gene            complement(1269..1574)
                     /gene="ccdB"
                     /locus_tag="ACHKG5_004376"
     CDS             complement(1269..1574)
                     /gene="ccdB"
                     /locus_tag="ACHKG5_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP0870352.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1576..1794)
                     /gene="ccdA"
                     /locus_tag="ACHKG5_004377"
     CDS             complement(1576..1794)
                     /gene="ccdA"
                     /locus_tag="ACHKG5_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP0870353.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2337..2936
                     /locus_tag="ACHKG5_004378"
     CDS             2337..2936
                     /locus_tag="ACHKG5_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870354.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            2933..3553
                     /locus_tag="ACHKG5_004379"
     CDS             2933..3553
                     /locus_tag="ACHKG5_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870355.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3550..3750
                     /locus_tag="ACHKG5_004380"
     CDS             3550..3750
                     /locus_tag="ACHKG5_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870356.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4158..5135
                     /locus_tag="ACHKG5_004381"
     CDS             4158..5135
                     /locus_tag="ACHKG5_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP0870357.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5420..6160)
                     /locus_tag="ACHKG5_004382"
     CDS             complement(5420..6160)
                     /locus_tag="ACHKG5_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0870358.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6281..6511)
                     /locus_tag="ACHKG5_004383"
     CDS             complement(6281..6511)
                     /locus_tag="ACHKG5_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP0870359.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
     gene            complement(6680..6964)
                     /locus_tag="ACHKG5_004384"
     CDS             complement(6680..6964)
                     /locus_tag="ACHKG5_004384"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0870360.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGATLQMMKSKHLK"
     gene            complement(6994..7134)
                     /locus_tag="ACHKG5_004385"
     CDS             complement(6994..7134)
                     /locus_tag="ACHKG5_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0870361.1"
                     /translation="MDSIKLLDKVIIYQQQNEQLYPAQEHLLLQLCMRVTKKLTDILM
                     PP"
BASE COUNT         1790 a         1839 c         1785 g         1728 t
ORIGIN      
        1 ataagtggat aaccccaagg gaagtttttt tcaggcatcg tgtgtaagca gaatatataa
       61 gtgctgttcc ctggtgcttc ctcgctcact cgagggcttc gccctgtcgc tcgactgcgg
      121 cgagcactac tggctgtaaa aggacagacc aaacatcatg gttctgtgtt cattaggttg
      181 ttctgtccat tgctgacata atccgctcca cttcaacgta acaccgcacg aagatttcta
      241 ttgttcctga aggcatattc aaatcgtttt cgttaccgct tgcaggcatc atgacagaac
      301 actacttcct ataaacgcta cacaggctcc tgagattaat aatgcggatc tctacgataa
      361 tgggagattt tcccgactgt ctcgttcgct tctcagtgga taacagccag cttctctgtt
      421 taacagacaa aaacagcata tccactcagt tccacatttc catataaagg ccaaagcatt
      481 tattctcagg ataattgttt cagcatcgca aactgcaccc ggtgccgggc agccacatcc
      541 agcgcaaaaa ccttcgtgta gacttccttt gaactgatgg acttatgtcc catcaggctt
      601 tgcagaactt tcagcggtat accagcatac agcatgtgca tcgcatagga atggcggaac
      661 gtatgtggtg tgaccggaac agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc
      721 tccccaatcc aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc
      781 cttcctgtgc gacggttacg ccgctccatg ggtattttca gtgttgccac catcgtctgc
      841 agctggctga cgtaccagga gtcagagagc ggaaccagcc ggtgagtctg ctgaccggcg
      901 ggcattctcc ccgccgtcct ggcagctttt tcggtccgtt gtttcagggt cgcaagctgc
      961 acaaacggat acggaggcgc aagcgaaaaa tccccccgcg tcagcgccag tgcttcatta
     1021 atgcgtgctc cggtgttcca cagtgtggcc agcagcatct tgcggtgcag attcgggacg
     1081 taatggagca gggcactcac ttccggagcc agcagatatt ttggcagttc atcatggacc
     1141 atcgacatct ggcgaagtgc cagagctgcc ggataatcaa tggcaacagg cagcgatgca
     1201 ggctgtccgg cagaatacac tgccggtacc atgactgcag actggctgtg tataacggag
     1261 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
     1321 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg
     1381 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact
     1441 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
     1501 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
     1561 accttaaact gcatttcacc agtccctgtt ctcgtcagca aaagagccgt tcatttcaat
     1621 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
     1681 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatgc
     1741 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcaca
     1801 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatgcc gcaaaaatca
     1861 gcgcgcaaat acgcatacta ttatctggct tttagtaagc cttatgtatt ttacctttcg
     1921 ttatgttaac caataaaaat taaaatctgc cttataaaaa caaagcgtaa ttaccgcatt
     1981 cccgtttcgt atggatccga ctttatttga ataaacgact ttccggaagt gtaccacctg
     2041 agaaaatcat catatcgctg ttttccgccc ctgcgcggcg catcacagtc cagagataca
     2101 acacaacata tagtgccatg ggcaactgaa tgaggcactg catgctgtgc tgacgggatg
     2161 taacgctgtg cggagggaga ggaagatccg taaccaccag aaaccggatc tgaccaggaa
     2221 aagagaaaga gtgtatgtaa gttctgtact gtatgtatga attgtattgt atgtattgtg
     2281 tatttttcat accgggcata cgatggcgcc attccctgag aaaccggagc cagaccatga
     2341 catccctctc cccggacacc gtccgccgca ttgaagacgc cgccgccgcc ctgatcgccg
     2401 ccggcacccc gaacccgacc aacgagcagg tccgtcagca cctcggcggc ggctccctgt
     2461 cccacatctc cccggtgatg cgcgcgttcc gcgcccgcca gcgcgagcag gccacgcccc
     2521 tgccgccgga gctggcgcag ctgctgaccg gccagctggg gctgctgtgg caggcggccg
     2581 tgaaacaggc tgaagccggg gccctggcgg cgcgcgagca ggccgacgac gacattgccc
     2641 gggccgacaa ggagcgggat gaggcgctgg cgaacgtggc cgcgctggaa agcgagctgg
     2701 cggtgctgcg cgaggtggtg gccgaacgtg accgcctgct gcaggaggta cgcgagctac
     2761 gcgcggaggc gctgccgctg cgcgagcagg tggcccgcct gaccgccacc ggcgaacacc
     2821 tggcggcgca gctgcaggac acgaaggcgg agctgaagga agcccgggag gacggccgcc
     2881 agcttcagac cgaactgctg gctctggccc gccaggacgg gaaggtgaag aaatgagaca
     2941 ggaaagagta ttacgtataa aggcatttcg acgggcactg gagcttggtg gcggtgaacg
     3001 ggctgacggg tgtcgtcatt ttccccgctg gaaaacggaa ctgaggaatt tcccgcacag
     3061 cagctgcgat ttagccagca atactctggc gtactacctc aaagaacgag agagatgcca
     3121 tccgtgcatc atttttatgg aagggaacgc tggctttcat gaagcggaaa actcaacagt
     3181 tcacgggcat gtcatcgtat tgctggacgg agagtacatc gatttgacgc tggatcaatt
     3241 cgacgaatat ccggactaca ttccttacga acctgtcgaa tctgacgggc agattggtaa
     3301 gctgttgcgt aatatcatga agcatgagga cccggtaaaa acccgccaga ttgatctcga
     3361 tggtggcgaa gagctgtatg cctggttacg cgataccgcc gacgaagtgc tggcagcaga
     3421 tccggaatgg caggcccggg aacggtcgat agaagaagca agggaagccg ctattacggt
     3481 gttcccgttt ctgtctgacg tgcaaaaaac tgagtgcgaa cagggcccgg acagtcagga
     3541 ggctgccaga tgaagattta tcattacact gacctgaacg gcctgaaggg catcatcgaa
     3601 tcaggctcac tgtgggcgac gcacttttcc tttctcaatg acagtaatga gctgactcac
     3661 ggcatgaact gccttgagaa tgccctgcag tacctgaggg acgactttaa cccgaaaacc
     3721 ctaaagttca tcgaacaggc acccttataa gcggttgtgt atgctgtttc cctggaatga
     3781 tttatccaca tatccggagg ctcgatccag taaacagatc catgaatgat caacaaagga
     3841 tccattaaag atccccatac cgctgcaaac cttgtcactc atgggccggg accacgatca
     3901 cataagcagt ggcatgttac tgataaactg taacatgcta atgataagct gtattcagta
     3961 atccatatac tgaagtaagt taatgacata aactatggtc agtacgccag actcagctgt
     4021 taaatacagg ctgcaggttt ttcttcagtc agttagcggg gctctgacac acgatttgct
     4081 gtttattctt ttactgtcca caggcaggag gctttctgga aaacgaaaat tcagacatca
     4141 aaaaactgtt cggcgaggtg gataagtcgt ccggtgagct ggtgacactg acaccaaaca
     4201 ataacaacac cgtacaacct gtggcgctga tgcgtctggg cgtttttgta ccgaccctta
     4261 aatcactgaa gaacagtaaa aaaaatacac tgtcacgtac tgatgccacg gaagagctga
     4321 cacgtctttc cctggcccgt gctgagggat tcgataaggt tgagatcacc ggcccccgcc
     4381 tggatatgga taacgatttc aagacctggg tggggatcat tcattccttt gcccgccata
     4441 acgtaattgg tgacaaagtt gaactgcctt ttgtcgagtt tgcaaaactg tgtggtatac
     4501 cttcaagcca gtcatcacgc aggctgcgtg agcgcatcag cccttccctg aaacgcattg
     4561 ccggtaccgt gatctccttt tcccgcaccg atgagaagca cacccgggaa tacatcaccc
     4621 atctggtaca gtcagcctac tacgatactg aacgggatat tgttcagtta caggctgatc
     4681 cccgcctttt tgaactgtac cagtttgaca gaaaggtcct tctccagctt aaggcgatta
     4741 atgccctgaa gcgacgggag tccgcccagg cactctacac ctttatagag agcctgcccc
     4801 gggatccggc accggtatcg ctggcgcggc tgcgtgcacg cctcaatctg aagtctcctg
     4861 tattttccca gaaccagacg gtcagacggg caatggagca gctgcgcgag attggatatc
     4921 ttgattacac ggagatccag cgggggcgga caaaactctt ctgcattcac taccggcgtc
     4981 cccggttaaa agcaccgaat gatgagagta aggaaaatcc gttgccacct tcacctgcgg
     5041 aaaaagtcag tccggagatg gcggagaagc ttgccctgct tgagaaactg ggcatcacgc
     5101 tggatgacct ggaaaaactc ttcaaatccc gctgaacata aactgtagtc agtgaagagt
     5161 gttcctttac tgactacagc ttatattatc aggtgcagtg agtggtctgc tcactgcagt
     5221 ttatattcag tttcctgcag tgctgcctgt agctgagctg tcatctgccg gtcccttacg
     5281 tgagtcaccc cgtaacctga tgctgaggca ttgctccctt cataaaacat gacttactca
     5341 ctacagctta tatacatgct ccagcttatg ttatgtctgt tctgctgacc acagcttata
     5401 taaggaagcg cgtatgccgt cacctcaggg gaggcagtgt acgcaggatc tccgcagcat
     5461 cccgcccgtc acctgtgaaa ggcactgcca gcgtggcagc catatccagc gcaaacactc
     5521 tggtataaac ctccatcgaa cgtggatccc tgtgacctgc cagggcctgg atgactttcc
     5581 ggggctggcg gtgatagagc atatgcatga tatagctgtg ccggaaggtg tgtggtgtga
     5641 ccggaatcga aaagtgtact ccgtcagctt cggcccgtct gacagcctgc ttcagccagt
     5701 tgcgcatggt ttcgtcggtc acggcccata atggttcacg acgacggggc cgggtggtga
     5761 tcatccagct ttccatctgc ctgacatagc ttatatccgt cagcggaacc aggcggactt
     5821 catcttttgg cgggcgtccg cgtcgcgcac gcactttttc ggacaggatc cgcacaaacg
     5881 gccttgctcc atccaggtca aatgattccg gcgtcagcat ccgggcttcg ccaatacgca
     5941 ttccggtatt ccagagggtg gcgaacagca tatggtgacg ctgatccggc atatagaaaa
     6001 gaagggcact cacttccggg gccagcaggt aggccggtgt ggcccctgtg gaagtggcca
     6061 ttcttctcag ggagagcgct gttgcaaaat ccacccccgg cgcaatgggt aacaggaaga
     6121 cgcgttctgg tgaattctgc aggggaatga cattgttcat gagttatttt tcacgtagca
     6181 cacgcactgt ttatggatac aggtattctg acctgaacgc ccggatcctt ccacgacaat
     6241 aaatcgcagc gtgcggatcc ttttgtttgc ttatcacgca ttattgcggg taaaaccaaa
     6301 tacgtaaagg tacgttttgc agcagggatc ccgtattaag ccagtcgtgg cgcgggctga
     6361 gggctggttt gccgtttttg ggagaattgg ggtaatgcca ggacgctaat gatctatcaa
     6421 tggatggatg taagtgacaa ttgaaaactg aaaaatgcgc tatgtagatt ctgacagatt
     6481 tatgcacctg aaatacgttc aaaatccaca tagcgtaact ctgattttcc ttaccagaaa
     6541 aaagaggggg aataaccagt atctatcccc cctacacaat tgcagaataa taaattataa
     6601 aaataagaac tcaggattta tatacatgat tacatgtaat attactgaga aatattttga
     6661 gataaaacct ttttgctgat tattttaaat gttttgattt catcatctgt aaggttgctc
     6721 catatttttt taaatagcgt ccatatatcc ttgtggtccg ctgtaaatac aaattaccgt
     6781 ctgaagtcag ataaatattc ttactacgtc gatcttcctt actttccata cgtttaataa
     6841 aaccaaattt ttccagagaa tcacaagctc tggtgatata tgttctggaa acatctaatc
     6901 tttcactgag ttcagtcgaa gataagcaga aattatcagt actggataac aaggctagaa
     6961 gcataagcgt tgtatcgttt ataccatccc ccttcaggga ggcattaata tatcagtaag
     7021 ttttttggta actcgcatac aaagttgtaa aagaagatgt tcctgtgctg gatataattg
     7081 ctcattctgt tgttgataga taataacttt atcaagtaat ttaatactat ccattccata
     7141 ac
//
LOCUS       DAZGXS010000185         7130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGXS010000185 DAZGXS010000000
VERSION     DAZGXS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..99
                     /locus_tag="ACHKG5_004386"
     CDS             <1..99
                     /locus_tag="ACHKG5_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870362.1"
                     /translation="IACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
     gene            142..702
                     /locus_tag="ACHKG5_004387"
     CDS             142..702
                     /locus_tag="ACHKG5_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870363.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            788..1489
                     /locus_tag="ACHKG5_004388"
     CDS             788..1489
                     /locus_tag="ACHKG5_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0870364.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1542..4064
                     /locus_tag="ACHKG5_004389"
     CDS             1542..4064
                     /locus_tag="ACHKG5_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0870365.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4080..5414
                     /locus_tag="ACHKG5_004390"
     CDS             4080..5414
                     /locus_tag="ACHKG5_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870366.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5411..6100
                     /locus_tag="ACHKG5_004391"
     CDS             5411..6100
                     /locus_tag="ACHKG5_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0870367.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6246..7028)
                     /locus_tag="ACHKG5_004392"
     CDS             complement(6246..7028)
                     /locus_tag="ACHKG5_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0870368.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1927 a         1739 c         1662 g         1802 t
ORIGIN      
        1 attgcttgtg atagtgatgg cttttacatt ttcaaggcac ttatacagtt aaggagattt
       61 accggtttta aaaacgtcag attgtatcaa ttgcattaat taactcgttc tatattaact
      121 ctaaatttca aggatattaa tatgttttct actttcaaaa aagcagctct gctggcagct
      181 attgcattac ctttttcaac tatggctgcg cctacagtca cttttcaggg tgaagtaacc
      241 gatcagacct gttccgtaaa tatcaacggt caaaccaatt cagtagtatt gatgccgacc
      301 gtagccatgg ctgacttcgg tgcaacttta gctgatggtc agagcgcagg ccagacgccg
      361 tttacggttt ctgtgtctaa ctgccaggct ccaactggtg cagatcaggc aatcaacacc
      421 accttcctgg gctacgacgt tgacgctagc acgggtgtta tgggaaaccg tgataccagc
      481 agcgatgcgg cgaaaggctt tggcattcag ttaatggatt ccagcacttc tggtaaccca
      541 gtaactctgg ctggcgcgac taacgtaccg ggtctgaccc tgaaagttgg cgataccgaa
      601 gccagctacg acttcggtgc gcgttacttc gttatcgata gcgctgctgc cactgccggt
      661 aaaattaccg ctgtcgcaga atacaccctg agctacctct aattaatcta ctggctggca
      721 gtaaatgcca gccagtattt cttcgcacaa ttaatagtga tttttcatgg acatggaaaa
      781 gataagaatg aacatttttg catatttact ggtacttgta ttttccatga gcatgagcag
      841 cagcgcgttt gccagcgtgg taatgaccgg aacccgtatt attttccctg gtgacgcaaa
      901 ggaaaaaacc atccagttgc gaaataccag cgatcagccc tatatcatta atatccatgt
      961 tgaggatgaa cgtggttctg acaagaatgt accgtttatg ccaaccccgc agacatttcg
     1021 catggaagct gccgcaggtc aggcgttacg cctgctctac actggtaata atttaccgca
     1081 ggatcgcgag tctgttttct ggtttagttt cagtcaacta ccttatctga ataagaatga
     1141 taaaagtcag aaccagctca tcctggccct gactaatcga gtcaaaattt tctatcgtcc
     1201 cagctcgatt gtcggtaaat ccagtgacgc acccaaaaac ctgacttacc aggtaaaaca
     1261 gaaccgcatt gaagtgacga atcccacggg ctattacgtc acaattcgcg ccgctgaact
     1321 gcttaataat ggtaaaaaag tccccctcgc gaattcggta atgattgctc ctcaaagcac
     1381 aactgaatgg acactaccct ctggcatcag tgtcgctccc ggtgcgcaga tccatttagt
     1441 gaccgtcaac gactatggcg taaatgttac gtctgagcat gccttataac ctctttctcc
     1501 ttttttgagc ggtatccttt ttcacgcacg agtacgtcga gatgaataaa tccgttgtgt
     1561 caatttctgc ggcaatgttg gttttacttt gccaaccggt catggggagc gaaatctcac
     1621 ccgcaacacc gtcagatgaa gacaactaca cctttgaccc gcaactcttc cgcggcagca
     1681 gatttagtca gtcgtcatta gcaaaactga caacacgtga gtctgttgca ccgggcaatt
     1741 ataaaatgga tatctacacc aacaataagt tgtcaggcag ttggaatgtc acgtttaaag
     1801 aagccgctga tggtcgcgtt ctgccctgcc tgacgcctga agtcgcggac gcgataggcc
     1861 tcaaaacagg ggaagataag ggggaaaaag atcctgtctg tacgtttgct aaggaactcg
     1921 ctcccggcat caccagccag acacagttgt cacaattgcg cctggactta tcggtgccac
     1981 agagtcaatt gattagtcgc cctcgcggct atgttccccc cagcgagctg gataccggag
     2041 catcgctggc gttcatgaat tatattgcca actattacaa cgttgcctat tcagggcaga
     2101 atgctcatag ccagcgttcg ctatgggcat catttaatgg tggcatcaac cttggtgcct
     2161 ggcaatatcg tcagttatcc aacatgacct gggataatga caaagggaat cagtggaaca
     2221 atattcgtag ctatttgcaa cgcccgctgc ccgccataaa tagccagtta atgatggggc
     2281 agcttatcac cagcggaaga tttttctctg gactcagtta tcacggcgtt agtctcgcga
     2341 ccgatgaacg tatgctgccg gactccatgc gcggctatgc gccgactatt cgcggcgtgg
     2401 ccgcaacaaa cgccagagtc tcggtaatgc aaaacggtca tgaaatatat cagaccaccg
     2461 tggctcctgg ccctttcgag ataaacgacc tataccccac cagctacagc ggcgatctgg
     2521 atgtcaccgt tacggaagct aacggcgcag tcagtcgttt cagtgtcccc ttttcagccg
     2581 taccagaatc gatgcgtcca ggaacttccc gttataacgt ggaagtaggt aaaacgcagg
     2641 atagtggtga tgactcgatg tttggtgacc ttacctggca gcacgggatg actaatacgc
     2701 tgacatttaa cagtggttcg cgtatcgctg atggctacca ggcgctgatg ctgggcggag
     2761 tctatggcag ttcgctgggg gcatttgggg caaacctcac ttggtcccat gcgcgtgttc
     2821 ccgaaagcga agcgcagagt ggttggatgt cgcaattaac ctggagtaaa actttccagc
     2881 ctacttcaac caccgtctcc ctggcaggtt atcgatactc taccagcggc tatcgtgatc
     2941 tggctgatgt gctgggagag cgtcatgctg ccagcaataa acagtcatgg gactccagcc
     3001 agtggcgtca acagtcgcgc ttcgatctta cgttaagtca gagccttgcg aattacggca
     3061 acctgtttgt gtcaggttca acacagaact accgtggcgg caagagccgt gatacacagc
     3121 ttcagttagg ttacagcaat agctttagcc atggcatcag tatgaacctt tccgtcggac
     3181 gccaaagaat gggcggctat aaagacaatt ctgatgatat gcagacggta acatcccttt
     3241 cattctcatt cccacttggc ggcaatggac ctcgtgtacc aagtcttagc aacagctgga
     3301 cccattcaac tgacggtagc tcgcaattac aaagctcgct aaccggaatg cttgatgaag
     3361 cacagaccac caactacagc ctgaacgtca tgcgcgatca acaatataag cagacgacgc
     3421 ttagcggaaa catgcaaaaa cgtttttcac aaactaccgt cggattgaac gcatcgaagg
     3481 gccaggatta ctggcaggct tcaggtaacg tacaaggcgc gatggctgtg catggtggcg
     3541 gcattacttt cggaccttat ctgggtgaaa cgttcgccct ggtcgaagct aaaggcgcag
     3601 aaggtgcaaa agtctataac tccagtcagc tggaaattaa tgacagtggc tatgcgcttg
     3661 ttccggcagt aacgccctat cgctacaacc gtatatctct cgatccacaa ggaatggatg
     3721 gcgatgccga gttggtcgac agtgaaagac aggtagcacc ggttgcgggt gcggcggtga
     3781 aagtaatttt ccgtacccgt cctggtaaag cgttgctgat taaatcccgc atggcagatg
     3841 gttcggaact gccaatggga gccgatgtgc tggatgagaa taatacagtc gtcggtatag
     3901 ccggtcaggg ggggcaaatt tacctccgca cagaacagac aaaaggccac ttgtcagttc
     3961 gctggggtga aggtgctaac gatagctgcc aattgccctt tgatatcagc gggaaggaca
     4021 gcaatagccc tatcatccgc ctgaatgaaa cctgtcagtc ttgatacaga gaaaacccga
     4081 tgtctaaatc gactttttta cacattctga ttagctccat catattggtg gcactaatac
     4141 aatcatccgc ttgggctaac tgtacaaaca cacagatagg tcaaactgaa gatggtcgga
     4201 cagcgcttat tgagttcgga aaaattaata tgaccgacac ctattttgcg ccagcaggtt
     4261 cactcctcgc gacaacggtc gtacctccca ctaactacac atcaggtggc gcgacaggaa
     4321 gctccgtatt gtgggaatgt gatgcaacag atttgccaaa catctatttt ctggtcgcta
     4381 ccaatggcga cgaccgcgtg gggggctttt acgatgcagg cgggcctgat ggtctgagtg
     4441 atgtctatgc cacctggttc gcttttgtcg gtctcaagca gaccatggcg ggcgtgacgc
     4501 ttggtcgtta ctggaagaaa gtgcccatca ccagttatgc cactcaggga actaaaatcc
     4561 agattcgctt acaggatatc cctcctcttc atgctgagct ttatcgcatc agtacgctac
     4621 ctgatacatc agcaacaaca agttggtgcg gtaataataa tacagatagt agtggagtcg
     4681 gattcgcaaa accttccggt acaatctata actgtgttca gcccaatgcc tatattcagc
     4741 tttccggtac cagcggcatt ttatttggtc atgatgagcc cggcgaagat agttctgttc
     4801 attgggattt ctggggtgct gataatggtt ttggttacgg aatgcgttcg gccaatcgac
     4861 tctacaacaa tgccacctgc gttgcccgca gcgccacgcc gttagtattg ctgccgacaa
     4921 ttgcagaagc acaactgaat gcgggcatgg aaagtaccgg taattttaat gtccgcgtcg
     4981 agtgtagtaa ctcggttcaa tcagggatta gcgatactca gacagcatta ggaatccagg
     5041 tgtctgaagg tgcatataca gcggcgcaaa aactggggat tatcaatagc aacggcggcg
     5101 tcagcgccct ggtctctgat aattatgacg cagcagagat ggcaaagggc gttgggatct
     5161 acatttctaa cagtgctcac cccgatacgg cgatgacgct ggttggtcaa ccgggcatcg
     5221 cgaagttaac ccccggagga aatgcagcgg ggtggtatcc tgtatttgaa ggggcaacat
     5281 tagaaggtgc gactcacccc ggatactcca gctatagtta ctcttttatc gcccggttga
     5341 agaaactgcc aaatcagaca gtcagtgcgg gaaaagtgcg ggcaacggct tatatattgg
     5401 tgaaaatgca atgaaatccg ttttgctgct cttaaccatc ttgttcgtac agtcagtgga
     5461 agccggcatc atgccggctt cttcacggat tatttatcgc gatggcgatc gggaaaagac
     5521 actgatgctg gtcaatacca atgactatcc ggtactggtg caaagttggg tagataatgg
     5581 tgaaggttcc ccagaagctg cttctccccc ctttgtggtg ctgccaccgg ttttccgcct
     5641 ggctgcgaga cgaacccagg ggttgcggat tatctataat catgacccac ttccccagga
     5701 tcgcgaatct gcattctggt taaatcttta tgaaatgccg ccagaaactc cccatcaaga
     5761 tcccacacat ttaacgctgg caatgaatac gcaacttaaa atcttttaca ggccaaaatc
     5821 tgtaactatg acgccccaag aagctatgcg taaaataagt tggcatctgg gtaacgacgg
     5881 cagcagtagc tatattgagc ttaataaccc aacaccactg aatatctctt tcacctccat
     5941 cagcattggg gctacaaagg tgcagcaaga aagcgatatg atgataaaac ccttcagtca
     6001 acgacggtac tggttagcaa gtaataacgt tagtggtgtg gttgcggcga catatctcga
     6061 tgataatggt gcacagataa aacaacaatt agcgcaataa tgctctgagt ggtatgtaaa
     6121 ctcatcgcga tggttcaacg ctaacgaata accactatac ccacctgaag taccggaagt
     6181 ggagaaatga caaaatctct ccactccatt ttccagtcaa aacacctcaa cgcagccaaa
     6241 aatcgttaac gaattgaata tccaccatca atgactaata gttgtccatt gacataatct
     6301 gaagcggcac tggcgaggaa caccattgcg cccataagat cctgtgccct tccccagcgt
     6361 tgagccggta tacgcccttt tattaactct tttattttag ggttattata aataatagcg
     6421 gtcatttcac tcacataata tcctggggcg ataccattaa tctgaatatt atattccgca
     6481 aaatcatcgg catacgcttt ggtcagcccc gctaaagcat gtttagtcgc cgcataccct
     6541 ggcgaccaac gtcccccatg aaatgacaat acggaacaca tattaataat tttccctttt
     6601 ctttgcggga caaatatatt caatgcggcc tggctcatat cgaatgcagc attcaggttg
     6661 agattaatca tcggatccca gtcgtttcga ttgaaatcga taggacgatt aatgttacac
     6721 acgcccgcgt tattgaccag aatatctaac tgaccgaaac gctccagaca cctattaata
     6781 atggtgcatg atgcatcagg ttttgttatg tctattgtca taaactcaac ttcaacgcct
     6841 tcatgagtta aatgcttcaa atatctatta tcaggctctt tgatgtcaac caacaataga
     6901 ttagcccctg ctttggccag agccgagcat aagcctctcc ccagctcacc cgcggcccca
     6961 gttacgatgg ccaccttgtt atgtaatgaa aaaaaatcca atgaaaaatc ttcaatagaa
     7021 aagctcatat cacacattgc tccttatgag taagtaatat ttaagggata atttaagtaa
     7081 tttttagatt tcaataaaaa aatataaaca ttaaaactca gcatgaataa
//
LOCUS       DAZGXS010000186         6912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGXS010000186 DAZGXS010000000
VERSION     DAZGXS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            157..1743
                     /gene="pdeC"
                     /locus_tag="ACHKG5_004393"
     CDS             157..1743
                     /gene="pdeC"
                     /locus_tag="ACHKG5_004393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP0870369.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1746..2069)
                     /gene="soxS"
                     /locus_tag="ACHKG5_004394"
     CDS             complement(1746..2069)
                     /gene="soxS"
                     /locus_tag="ACHKG5_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0870370.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2155..2619
                     /gene="soxR"
                     /locus_tag="ACHKG5_004395"
     CDS             2155..2619
                     /gene="soxR"
                     /locus_tag="ACHKG5_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0870371.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3165..4514
                     /gene="ghxP"
                     /locus_tag="ACHKG5_004396"
                     /gene_synonym="yjcD"
     CDS             3165..4514
                     /gene="ghxP"
                     /locus_tag="ACHKG5_004396"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0870372.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4665..6314
                     /locus_tag="ACHKG5_004397"
     CDS             4665..6314
                     /locus_tag="ACHKG5_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0870373.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6468..>6912)
                     /gene="espX5"
                     /locus_tag="ACHKG5_004398"
     CDS             complement(6468..>6912)
                     /gene="espX5"
                     /locus_tag="ACHKG5_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP0870374.1"
                     /translation="FKHADLSRVNLNKAILQNINLIDSKLFFTRLTNTFLEMVICTDS
                     NMANVNFNNANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASVQGMGISILRGEENIPI
                     NSDTLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQHAD"
BASE COUNT         1559 a         1601 c         1828 g         1924 t
ORIGIN      
        1 atcaatgaat tattttcatc caagtgacga ttaaaaaggt atcaatttca aatcaggcaa
       61 aagtgctatt tataccgtaa gatttatcta aagacgtcgg tagccagggt ttcaccttgc
      121 aatggccggg tataaacagg caggaaattg atagcaatga atcatcgtgc acgacaccaa
      181 ttactggcgt tgccgggcat tatcttttta gttctctttc ccatcattct ttcgctatgg
      241 attgccttcc tttgggcaaa atcagaagtg aataatcagc tccgaacctt tgctcaactg
      301 gcgttggata aatccgagct ggtcattcgc caggcagatt tagtgagcga tgcagctgaa
      361 cgctatcagg ggcaagtttg cactccagcc catcaaaagc gaatgttgaa tattattcgt
      421 ggctatcttt atattaatga attgatctat gcccgtgata atcatttttt atgctcatcg
      481 ctgatagtgc ctgtaaacgg ctatacgatt gtatcggccg attataagcg tgaacctaat
      541 gtttctatct attattaccg cgatacgcct tttttctctg gctataaaat gacctatatg
      601 cagcggggaa attatgtggc ggttatcaac cctctcttct ggagtgaagt gatgtctgat
      661 gacccgacat tgcaatgggg tgtgtatgat acggtgacga aaaccttttt ctcgttaagc
      721 aaagaggcct cagcagcaac gttttctccg ctgattcatt tgaaggattt aaccgtacca
      781 agaaatggct atttatatgc gacagtttat tcgacaaaac gcccaattgc agccattgtt
      841 gcgacttcat atcaacgtct tatagcccat ttttataatc atcttatttt tgcgttgccc
      901 gccggtattt tggggagtct tgttctgcta ttactctggc tacgtattcg acaaaactat
      961 ttgtctccca aacgcaaatt gcaacgcgcc ctcgaaaaac atcagctatg cctttattac
     1021 cagccaataa ttgatatcaa aacagaaaaa tgtatcggcg ctgaagcgtt gttacgttgg
     1081 cctggtgagc aggggcaagt aatgaatccg gcagagttta ttcctctggc agaaaaggag
     1141 gggatgatag aacaggtaac tgattatgtt attgataatg tcttccgcga tctgggcgcg
     1201 taccttgcaa cacatgctga tcgctatgtt tctattaatc tgtcggcctc cgattttcat
     1261 acgtcacggt tgatagcgcg aatcaatcag aaaacagagc aatacgcggt gcgtccgcag
     1321 caaattaaat ttgaagtgac tgagcatgca tttcttgatg tcgacaaaat gacgccgatt
     1381 attctggctt tccgccaggc tggttacgaa gtggcaattg atgattttgg tattggctac
     1441 tctaacttgc ataaccttaa atcattgaat gtcgatattt tgaaaatcga caaatcgttt
     1501 gttgagacgc tgaccaccca taaaaccagt catttgattg ctgaacatat catcgagctg
     1561 gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg aaactgagga gcaggttaac
     1621 tggctgcgca aacgcggcgt gcgctattgc cagggatggt tctttgcgaa ggcgatgccg
     1681 ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc gggagttaac gcgcgggcaa
     1741 taaaactaca ggcggtggcg ataatcgctg ggagtgcgat caaactgccg acggaaaacg
     1801 cgggagaagg tctgctgaga gacataaccc aggtccattg cgatatcaaa aatcggacgc
     1861 tcggtggtgc gcaactcaac ggcggccagt aacagacggc gttgacgaat gtaatcgcca
     1921 agcgtctgat gcgtcaccgt gcggaacatc cgttgcaggt accactttga atagcctgat
     1981 ttttttgcga cgacatcaat gttaagcggc tggtcaatat gctcgtcaat ccatgcgata
     2041 agatcctgaa taattttctg atgggacata aatctgcctc ttttcagtgt tcagttcgtt
     2101 aattcatctg ttggggagta taattcctca agttaacttg aggtaaagcg atttatggaa
     2161 aagaaattac cccgcattaa agcgctgcta acccccggcg aagtggcgaa acgcagcggt
     2221 gtggcggtat cggcgctgca tttctatgaa agcaaagggt tgattaccag tatccgtaac
     2281 agcggcaatc agcggcgtta taaacgtgat gtgttgcgat atgtcgcaat tatcaaaatt
     2341 gctcagcgta ttggcattcc tctcgcgacc attggtgaag cgtttggcgt gttgcccgaa
     2401 gggcatacgt taagtgcgaa agagtggaaa cagctttcgt cccaatggcg agaagagttg
     2461 gatcggcgca ttcatacctt agtggcgctg cgtgacgaac tggacggatg tattggttgt
     2521 ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg gcgaccgctt aggagaagaa
     2581 ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa gcgccacaag ggcgctttag
     2641 tttgttttcc ggtctttgtc tttctctcta tcccgctggt acacaggagg gtttcccccg
     2701 acgtcaacac acctcattcg agcacgtggt ggaggttccg gttggtgttg atgctttaat
     2761 tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac tttttagccg caatattttt
     2821 gtcatctcag acgatttttt atcgcaatcc tcaacggtat acggctcgat aacgctgcaa
     2881 tcttgcgcat cgacgataac gtttgcgcat caattgcctg gtttttcatc gtcaagacaa
     2941 taaaagagaa aaaagcagca aacttcggtt gaaaaagccg ctatgatcgc cggataatcg
     3001 tttgcttttt ttaccacccg ttttgtatgc gcggagctaa acgtttgctt ttttgcgacg
     3061 cagcaaattg tcgcaaacct ggagcaggaa gataacgttt cgctggcagg ggattgtccg
     3121 ccacgcatct tgacgaaaat taaactctca ggggatgttc tcttatgtct acgccatcag
     3181 cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc acaacgtgga agcactgtcc
     3241 gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat ggtctactcg gttatcgtcg
     3301 ttccaggcat gttgggtaaa gcgggcttcc cgcctgcggc agttttcgtt gcgacctgtc
     3361 tggttgccgg actcggttct atcgtgatgg gtctgtgggc taatctgccg ttggcgattg
     3421 gttgcgccat ctccctgacg gcgtttaccg cattcagcct ggtgctgggg caacatatta
     3481 gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt gctgtttacg gtaatttctg
     3541 ccacgggtat ccgtagctgg attttgcgca acttgccgca cggtgtggcg cacggcacgg
     3601 ggatcggtat cggtctgttc ctgctgctca ttgccgctaa cggtgtcggt ctggtgatta
     3661 aaaacccgct tgatggtctg cccgttgcgc tgggtgattt cgcgaccttc ccggtgatta
     3721 tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa actgaaagtc cctggtggca
     3781 ttctgctgac cattatcggt atctcaattg tcggtttgat cttcgatcct aacgtccatt
     3841 tctccggcgt tttcgccatg ccttcattga gcgatgaaaa cggcaattca ctgattggca
     3901 gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc aagcgttctg gcgctggtga
     3961 tgacggcagt atttgatgcc accggaacta tccgcgctgt cgccggccag gcgaacctgc
     4021 tggataaaga tgggcagatc atcgacggcg ggaaagctct gaccactgac tccatgagca
     4081 gcgttttctc tggcctggtg ggtgcagctc cggcagcggt atacatcgag tctgcggcgg
     4141 gtacggcggc gggcggtaaa accggtttga cggctatcac cgttggcgtg ctgttcctgc
     4201 tgattctgtt cctctctccg ctttcttacc tcgttccggg gtatgcaacg gctccggcgc
     4261 tgatgtacgt tggcctgctg atgctgagca acgtggcgaa aatcgacttt gctgattttg
     4321 ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt gctgacctgt aacatcgtaa
     4381 caggcatcat gatcggcttc gcgactctgg tgattggtcg tctggtttcc ggtgaatggc
     4441 gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct ggtgaccttc tatgcgggtg
     4501 gctgggctat ctaatctttc atcggattct gaaaacgggt ggcaatggct gcccgttttt
     4561 attttctccg cacattgtgt gagcttttgc gatattctga aaaaatgaga attcaggcat
     4621 aacgtctttc tcagatcata aaaaacatcg caaacaggga acgcatggaa atcttcttca
     4681 ccatactgat aatgaccctc gtggtctcgc tgtccggggt ggtcactcgt gtcatgccct
     4741 ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc gctactggcg tggccgacgt
     4801 ttggtttgca tgtggagttt gatcctgaac tctttttagt cttgtttatc ccgccgttgc
     4861 tgttcgctga tggctggaaa acgccgaccc gtgaatttct cgaacatggt cgagagattt
     4921 tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg cattggcttc cttatttact
     4981 gggtggtgcc gggcattccg ctgatccccg cctttgcgct ggcggcggtg ctttctccga
     5041 ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg catcccgaaa aaaatcatgg
     5101 gcattttgca gggcgaagcg ttgatgaatg acgcctccgg cctggtgtcg ttgaagtttg
     5161 ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg cggtgcgacg gtcgaattta
     5221 tgaaagtagc cattggcggt attctcgccg gttttgtggt gagctggctg tacggtcgct
     5281 cgctgcgatt cctcagccgc tggggcggtg atgaacccgc gacgcagatc gtcctgctgt
     5341 tcctgctgcc attcgcttct tatctgattg ccgaacatat tggcgtttcc ggcatcctcg
     5401 ctgcggttgc cgccgggatg accatcaccc gctccggtgt gatgcgccgt gcgccgctgg
     5461 caatgcgcct gcgtgcaaac agcacctggg cgatgctgga atttgtcttt aacggcatgg
     5521 tgttcctgct gttaggtctg cagctgccgg gtattctgga gacgtcgctg atggcagcag
     5581 aaatcgaccc taacgtcgaa atctggatgc tgtttaccga tattattctg atatatgcgg
     5641 cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa aaagttcagc aaccgcttcc
     5701 tgaagaagaa gccgatggag tttggttcgt ggaccacacg agaaatcctg atcgcgtctt
     5761 tcgccggggt gcgtggggcg atcactttgg ccggtgtgct ctctatcccg ctgctcttgc
     5821 cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt tctggcggct ggtgtcattc
     5881 tcttctcgct gtttgtcggc gtggtgatgt tgcctattct gctacaacac attgaagtcg
     5941 cggaccattc gcaacaattg aaagaagaac gtattgcgcg agcggcaacg gcagaagtgg
     6001 cgattgtggc gatccagaaa atggaggagc gtctggcggc ggataccgaa gagaatatcg
     6061 ataaccagct gcttacggag gtcagttctc gcgtcattgg taacctgcgt cgtcgcgccg
     6121 atggacgtaa cgacgttgaa agttccgtgc aggaagagaa ccttgagcgt cgcttccgtc
     6181 tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct gcgcgccacg cgggagatca
     6241 gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt gcttgaagcg ttgctaattg
     6301 aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga ggtaatctga aaaaggtgca
     6361 atcttttgca cctttaacag gttagctcgg gctaacattc atgattctaa aacaaataag
     6421 ttatctctct tttaaagtca tttcattggc tgttcttcga cttaatctca atctgcgtgt
     6481 tgcataatat ctgcagtaat cttcatagct acttcatgag tattattctc agtttgtgac
     6541 atgccagtat gagaggtaca atcttcttca aagaatttct gtagtgttac cagggtatca
     6601 ctattaatgg gaatattctc ctccccacgg agaatggaaa tgcccattcc ctggacgcta
     6661 gcctcatcga aattaacccg atagagacgg gtattaaaca tccaggcttt tgttaaaaca
     6721 gaacagttga aatggcaatt gtttaaattg gcattattaa aattaacatt agccatatta
     6781 gaatcggtgc atataaccat ttcgaggaac gtatttgtta gccgtgtaaa aaagagttta
     6841 ctatcaatta agtttatatt ctggaggatt gctttgttca gattgacgcg agacagatca
     6901 gcatgtttaa ag
//
LOCUS       DAZGXS010000187         6859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGXS010000187 DAZGXS010000000
VERSION     DAZGXS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHKG5_004399"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHKG5_004399"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0870375.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(858..1694)
                     /gene="kduI"
                     /locus_tag="ACHKG5_004400"
     CDS             complement(858..1694)
                     /gene="kduI"
                     /locus_tag="ACHKG5_004400"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP0870376.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1981..3162)
                     /gene="yqeF"
                     /locus_tag="ACHKG5_004401"
     CDS             complement(1981..3162)
                     /gene="yqeF"
                     /locus_tag="ACHKG5_004401"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0870377.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3417..4646
                     /gene="yqeG"
                     /locus_tag="ACHKG5_004402"
     CDS             3417..4646
                     /gene="yqeG"
                     /locus_tag="ACHKG5_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0870378.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5106..5738
                     /gene="yqeH"
                     /locus_tag="ACHKG5_004403"
     CDS             5106..5738
                     /gene="yqeH"
                     /locus_tag="ACHKG5_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870379.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            6072..>6859
                     /gene="yqeI"
                     /locus_tag="ACHKG5_004404"
     CDS             6072..>6859
                     /gene="yqeI"
                     /locus_tag="ACHKG5_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0870380.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLS"
BASE COUNT         1950 a         1599 c         1416 g         1894 t
ORIGIN      
        1 ttatgatgat ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg
       61 aatgaattaa cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc
      121 tgaagcgctg gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg
      181 gccagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg
      241 ggtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcattc gccatcaatc gcgtcacgcc catcacgccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
      421 attgccttgc gcgatgaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcctt ttcgctgaac tcgagagcat cttcgcggcg
      541 aatcaatccg gcgttattca ccaggatatc aatatgacca aactcagcta ccgcgcgatc
      601 cagcagtcca ggaataccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct tgatggtttc agtcggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag
      901 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
      961 tgaatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
     1021 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
     1081 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacccgga
     1141 gccagctccg tcagccccat actcaattgg caggtttcca gcacatccgg gacaaaatat
     1201 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
     1261 tccggtgtga cttttttggt aggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
     1321 ttcgccggag tgccggtatc aatactagca aagacaactt cttttgcgcc tttacccaca
     1381 tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca
     1441 ccgccaatat tgataacacc taactcgcga cgttcaagga aataacttac gccgagttgt
     1501 ttacccactt ccccgccaac ggaaaccgtt ttagttaccg gcataatgcc gccaataata
     1561 atacggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
     1621 aaaaattcat tgcgcagccc ctgggtttcc agcgtttttg cgtgcgcact gtggatgctc
     1681 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
     1741 gaaacactgt ttcgttttct ctataagggg atgatatgcc catgggatcg ggttttcaat
     1801 taaaaataaa acaatgtttc atttttaagt taaggattaa aaaaagtgca gtgtcgatca
     1861 cgaatctgtc atgagaatgg aaaaatcagg gagcccggaa aatgtatgtc gacttgccag
     1921 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
     1981 ctattcgtca cgttcaatgg tcaatgccac accctggccc ccgccgatac aaagcgttgc
     2041 cagtcctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
     2101 accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg
     2161 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
     2221 ctcgataaga tcaacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
     2281 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat
     2341 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
     2401 agctgcgcca tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa
     2461 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgtgtggct gttcatcggt
     2521 atcaacaacc aacgtctgac cgttactttg ggtcattacc gggacgatct catctttaaa
     2581 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
     2641 caattgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
     2701 atcattgaag gcatcccata acccatcatg cacaagactg tcaaccagct ggctattgcc
     2761 aagctgtgca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
     2821 gccaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
     2881 tgctttaagc ccggaaccgc agacgtcatt aatcgtgatt gcagaaacgc tattaggcag
     2941 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
     3001 accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt
     3061 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccgc gaaagcagcc
     3121 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
     3181 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
     3241 ttttatcaca aaggaaatat acctgagcag tagtcagagt cataactggc acgtaaggtt
     3301 tgcaaccact accccaccaa tagaggggta gatagggcgt taatctccca tacttaacct
     3361 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
     3421 ctaatatttg gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg
     3481 gtgcaggcac gctcttcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
     3541 ttactgctct ggtcgcctgg cctttaacat actggccaca taaagcttta tgccagttca
     3601 tcctctcatc gaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg
     3661 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
     3721 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
     3781 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaattctg aatctcattt
     3841 ttctgatggg acgtcatgcc actattcggg taatgggatt tttggtattc ccattgattg
     3901 cctatttctt atttctttcc atttaccttg taggtagttg gcaacctgat ctattaacaa
     3961 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
     4021 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
     4081 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
     4141 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg
     4201 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
     4261 cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
     4321 tgggtacgta ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca acactacagc
     4381 aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc
     4441 tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tatgcgatca
     4501 gcggcccgct cattgccatg atacttttca tcatgcctac gctatcaaca tatctcatcc
     4561 cggcgcttaa accctggcgc tccatcggaa atctgattac gctgatcgtg ggtatcctgt
     4621 gcgtatcggt aatgttcttt agctaatatc cattgtgaat ggttcaaaat tgctacaatt
     4681 ttgaaccatt ccagttttca taatgtgcat ttgttattat tccgctctat cacttctctc
     4741 gtcatatcct agtattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
     4801 gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt
     4861 tgcgatatag atattctcat accatgagat agttatctga accaccctca accaggtaag
     4921 attaaatcag tgaaacataa tcatatttaa taacgagaaa tgcattttaa aatgtcttaa
     4981 tcagaattat agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcatg
     5041 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
     5101 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
     5161 gccaaataat gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgtttttgta
     5221 tgtggaccga agaaagttca tattttaatc gtggtgtggt agaaggtatc ttaacaaaaa
     5281 accacaatgc gagattaagc ggatatattt ttgtcgattt ttcagtaagt tttttacgtc
     5341 tattccttga aaaagactgg attgactatc ttgcaagtac tgacatgggg attgttttag
     5401 tcagcgaccg caatatgcaa tcgttagcta attactggcg aaaacacaac tctgcaatat
     5461 ctgctgtcat atataatgat gatggtcttg atgttgccaa tgaaaagatc agacaactat
     5521 ttattggtcg ttatttatca tttaccagag gtaacacatt aactcagatg gaattcacca
     5581 taatggggta tatggtttct ggttataatc catatcagat tgctgaagtt ctggatatgg
     5641 atatccgtag catctacgcg tacaagcaac gaatcgaaaa gagaatgggt ggtaaaataa
     5701 acgaattatt tattcgttca cattcggtcc aacattgata ctcaacaatc tgccagaatc
     5761 atactctgtt tatacgggca tttattgaaa tgcccgtatt ttataaatag gccgtcccct
     5821 caagagtaaa caccatagta tttaaaacca tcacacgaaa aattcagaag cattacgaaa
     5881 tatgaactta caaacaatag tggcataaat gttaaccatg ttaatttaca taaaatttta
     5941 cgttgcgaca ttaaagcctc atttcaatca tcatgataaa tataaaatta atatatattt
     6001 atgccgtaaa taccaatata cttagcaaac tatgtgattt ccatttcgat tgatttagtg
     6061 tttattgacg tatgtactgg attattaacg ataatatcga gttctggcct gagcaccgaa
     6121 aattaatatc ggtacataac gccgatctta acgtcgttct gacaacgcca gccagtcgtt
     6181 gtttatcact gctacttgaa gcttttcctg atgtggttgc acaacaagat tttttcacca
     6241 gagtctggga agaagaaggt atgcgtgtgc ctactaacac gttatatcag aacatatcca
     6301 ttatcagacg cggatttcgc gctgttggtg atactaccca ctcgcttatt gcaaccgtgc
     6361 cgagaagagg attcaagatc cataatgaca ttaacataca aaatcatgta ataaactcat
     6421 caacagacgc acatacacac aatgccccac ctgccataaa agttaatgcg ggatacaaag
     6481 agagcattgg tggcgcaaag aatttcaata acaaaatcct caaacatata aaaagttatc
     6541 taattatgtt gagcgcattt gtcataggcg catattctgc atattggcta tggaataata
     6601 atcaaccgaa gccatttttc aaggattaca agactgttgc ggaaattaat ggctgccatt
     6661 ttaatgtaac agaagataca attgatgggt tgaaagagtt cgataaatat aaaacacgga
     6721 tactggattc ggggattaac tgcaaaaaat atccgtggtt atacttccct cttgcaaaat
     6781 cctcaccggg gatgattgtt atggcatgca ataaaaacta taaccaacat gaagtggcag
     6841 gctgtttaac cctgtctta
//
LOCUS       DAZGXS010000188         6795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGXS010000188 DAZGXS010000000
VERSION     DAZGXS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(179..664)
                     /gene="sixA"
                     /locus_tag="ACHKG5_004405"
     CDS             complement(179..664)
                     /gene="sixA"
                     /locus_tag="ACHKG5_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0870381.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(867..3011)
                     /gene="fadJ"
                     /locus_tag="ACHKG5_004406"
     CDS             complement(867..3011)
                     /gene="fadJ"
                     /locus_tag="ACHKG5_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0870382.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3011..4321)
                     /gene="fadI"
                     /locus_tag="ACHKG5_004407"
     CDS             complement(3011..4321)
                     /gene="fadI"
                     /locus_tag="ACHKG5_004407"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0870383.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLCSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4501..4785)
                     /locus_tag="ACHKG5_004408"
     CDS             complement(4501..4785)
                     /locus_tag="ACHKG5_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0870384.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5157..6497
                     /gene="fadL"
                     /locus_tag="ACHKG5_004409"
     CDS             5157..6497
                     /gene="fadL"
                     /locus_tag="ACHKG5_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0870385.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1702 a         1835 c         1605 g         1653 t
ORIGIN      
        1 aaaatacatt acaacttagt ctcatttcgt cagaaattaa tagggaagca gaggaaaaag
       61 tgcgatgaat tctgtatcga agcgtatcaa ctccgtgaag ggttttgagt gaatttctac
      121 tattgcaaaa agacaaatgc ccgatgtaca gcttacatcg ggcatttttt caaacgaatc
      181 agatagcttt cgccatcttc aaattacatg ggctcatctg ccagttgaac tcgccttttc
      241 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      301 ccggacataa ctcggcgacc agatacccaa ctaacggtaa gtgggagata accaacaccg
      361 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      421 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tccccaactt
      481 cctccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      541 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccgtta gtggtcagag
      601 gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      661 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      721 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      781 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacaatc cgccattcag
      841 cgcggattca tatagcttcg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
      901 ctttccccac gcgcacccat ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac
      961 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1021 cgaaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
     1081 tcacgcacgc tacggataac ctgctcatca acacaacgta cagcttcatt cagcatcaac
     1141 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1201 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1261 tagaaacctc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1321 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1381 ccggtgtcga ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc
     1441 actagcgcgg catcaatgtg cgctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1501 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1561 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg tgctgatgta
     1621 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1681 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac
     1741 gtattcgaag caaagatggt atgagcggcg caattttgct caacttccgc caccatctgt
     1801 tgtttcaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1861 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1921 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     1981 ccctgcgggt tgatatcttt aattctgacc ggaatccccg ctttacaagc agtgacataa
     2041 gcaataccgc cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc
     2101 ggcgcatcac tgccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa
     2161 gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca
     2221 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2281 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2341 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2401 ttctttgcca gctcaacagc ggcttccagc agaatggagt gcggaacaac atcatccacc
     2461 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2521 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2581 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2641 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2701 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct
     2761 tccgcttctt gcgccgtttt gcaattgccg atcatgttga tgtctgcgcc agcaatgaag
     2821 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     2881 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     2941 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
     3001 gtcatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3061 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3121 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3181 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac
     3241 gttcactacc cagtagctga atattggcca acgtctgggc ggcaaaggct tcgtgcatat
     3301 caatcaatgt cagatctgcc attgtcaaac cggcacgctc cagcgccagc ggtgttgacc
     3361 aggctggacc gagcaacatg tcctgccaga catcgatagc ggtaaatgcg tagctgcaca
     3421 gatatcccag cggtaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3481 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttacgat
     3541 caaacgccgg acgcagcttt gcgaaatcag caagcgagga attaccgcga atattgttgt
     3601 cttccgcgag cagttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3661 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3721 gttctcgggt gatgccgtag gttttcgcca tttgctctgc agtgtcgccc atccgcaagc
     3781 cggtagaata ttctgctacc gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3841 agaagagttt cagacgctgg ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg
     3901 ccagtttttt actgacgcca attggcaata ccgaagagga atctgcccca ccggcaatcc
     3961 ccgctcggat agtccccgcc atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg
     4021 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattcca gtaccgagaa
     4081 caatttcacg cgcaatgttg ggagcttcag gcatttgtac gacctgacca aagaccagtt
     4141 gttcaatcac ttcggcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4201 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4261 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4321 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4381 tttttacatt tagccaagcg gagaaaaggg aaattgggag ctatgacaca gtgaagacag
     4441 gagaaagaaa agaaaacgcc cctacctgtt gctggcagga gcgtacttat ccgagaggaa
     4501 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4561 atccaggcgc acaccatcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4621 ttccacgcta cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4681 tgcaaacacg cggctgtaag acgctgtgca gtcggagttg tccataatgg tgccaacatc
     4741 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4801 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4861 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4921 aaaacaaaat tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc
     4981 gtttcttaga tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg
     5041 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5101 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5161 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5221 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5281 attcagggga aggcgcaatt gccgatgatg caggtaacgt cagccgtaac cccgcattga
     5341 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa
     5401 atatcagcgg aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5461 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5521 ctattacctc taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg
     5581 tcgggggtac aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5641 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5701 gtttcgcagg cgatctgggg cagctggttg ctggtcagat tatgcaatct cctgccggga
     5761 agactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5821 tcgctcatct gaacggcaac cagtggggct ttggatggaa cgccggtatc ctgtatgaac
     5881 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     5941 gtaactacag cagcgatctt aatcgtgtgt ttaataacta cggtttgcca attcctaccg
     6001 ccacaggtgg cgcaacgcaa tcgggttatc tgactctgaa cctgcctgaa atgtgggaag
     6061 tgtcgggtta taaccgtgtt gatccgcagt gggcgattca ctatagcctg gcttacacca
     6121 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6181 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6241 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gcacagaatc
     6301 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcgt
     6361 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6421 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6481 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact
     6541 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
     6601 cagcctgatg gcagctagcc ctttcaaatc agtaagtggt atccacagac aaacctgaag
     6661 ggaaacgcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt
     6721 tccaggatac tcaaccccct ggctattgtg cgctcataca cgcaaattaa agataggttc
     6781 taaataaatg agcgt
//
LOCUS       DAZGXS010000189         6791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGXS010000189 DAZGXS010000000
VERSION     DAZGXS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            257..1414
                     /gene="acrE"
                     /locus_tag="ACHKG5_004410"
     CDS             257..1414
                     /gene="acrE"
                     /locus_tag="ACHKG5_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0870386.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1426..4575
                     /gene="acrF"
                     /locus_tag="ACHKG5_004411"
                     /pseudo
     CDS             1426..4575
                     /gene="acrF"
                     /locus_tag="ACHKG5_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            4828..5049
                     /locus_tag="ACHKG5_004412"
     CDS             4828..5049
                     /locus_tag="ACHKG5_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870387.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5480..6505
                     /locus_tag="ACHKG5_004413"
     CDS             5480..6505
                     /locus_tag="ACHKG5_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0870388.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6573..>6791
                     /locus_tag="ACHKG5_004414"
     CDS             6573..>6791
                     /locus_tag="ACHKG5_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0870389.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLID"
BASE COUNT         1667 a         1623 c         1721 g         1780 t
ORIGIN      
        1 aagatacatt cactacatca atatatactt caatttacga ttttttaatt ctgcctcttt
       61 caacccgcgt caaaataaaa cagtagaata ttaatctttt tttgtgttta tgtgccttga
      121 gacgcctgta ttcataacta ttccttacat cgacgaatga taatttgtag gatagcgaac
      181 tgtatttttc tttctgcgag ttaacgcgtt gcctttttgg gtaaataacg cgcttttggt
      241 tttttgagga atagtaatga cgaaacatgc caggtttttc ctcctgccct cctttattct
      301 gatctccgcg gctttaatcg ccggttgtaa cgataaggga gaagagaaag ctcacgtcgg
      361 tgaaccgcag gttaccgttc atattgtaaa aacggccccg ttagaagtta agactgaatt
      421 accaggccgc accaatgctt atcgtatagc cgaagttcgc ccacaggtta gcgggatcgt
      481 actgaatcgc aatttcactg aaggcagcga tgtgcaagca ggccagtccc tgtaccagat
      541 cgatcccgcg acctatcagg caaattatga cagcgcgaaa ggcgaactgg cgaaaagtga
      601 agccgccgcc gccatcgcgc atttgacggt aaaacgttac gttccgctcg tgggtacgaa
      661 atacatcagc cagcaggagt acgaccaggc cattgctgat gctcgccagg ccgatgccgc
      721 cgtgattgcc gcaaaagcca cagtcgaaag cgctcgcatc aatcttgctt ataccaaagt
      781 gactgcgcca attagcggac gtatcggcaa atcgactgtg accgaaggcg ctcttgtcac
      841 taatgggcaa acgactgaac tggcaactgt ccagcagctc gatcctatct atgttgatgt
      901 gacccaatcc agcaacgatt ttatgaggct gaagcagtcc gtagagcaag gaaatttgca
      961 taaggaaaac gccaccagca acgtagagtt ggtcatggaa aacggtcaaa cctatcccct
     1021 gaaaggtacg ctgcaattct ccgatgtgac cgttgatgaa agcaccggct ccataaccct
     1081 acgtgctgtc ttccctaacc cgcaacatac gcttttgccg ggtatgtttg tgcgtgcacg
     1141 gattgatgaa ggcgtccaac ctgacgccat tctcatcccg caacaaggcg ttagccgcac
     1201 accgcgtggt gatgcaaccg tgctgattgt taacgataaa agtcaggtgg aagtgcgccc
     1261 tgtcgttgcc agtcaggcga ttggcgataa atggttgatt agcgaaggtc tgaaatctgg
     1321 cgatcaagtc attgtcagcg gcctgcaaaa agcgcgtccg ggagagcagg ttaaagccac
     1381 taccgatacc cccgcagata ctgcatcgaa gtaaggtaat ctgacatggc aaactttttt
     1441 attcgacgac cgatatttgc atgggtgctg gccattattc tgatgatggc gggcgcactg
     1501 gcgatcctac aattgcccgt cgctcagtat ccaaccattg caccgcctgc ggtttctgtt
     1561 tcagcaaact atccgggcgc tgatgcgcag accgtgcagg atacggtgac gcaggttatc
     1621 gaacagaata tgaacggtat cgataacctg atgtatatgt cctccaccag cgattccgcc
     1681 ggtagcgtga caattaccct taccttccag tccgggaccg atcctgatat cgcgcaagtg
     1741 caggtgcaga acaaactcca gctcgccacg ccgttgctgc cgcaggaggt tcagcagcag
     1801 gggatcagtg ttgaaaagtc cagtagcagc tatttgatgg tggcaggctt tgtctctgat
     1861 aacccagaca ccacacagga cgatatctcg gactatgtgg cctctaacgt taaagatacg
     1921 cttagccgtc tgaatagcgt cggtgacgta cagcttttcg gcgcacagta tgcgatgcgt
     1981 atctggctgg acgccgatct gctaaacaaa tataaactga caccggttga tgtgattaac
     2041 cagttgaagg tacagaacgc tcagatcgct gccggacagt tgggcggaac gccagcgtta
     2101 ccagggcaac agttgaatgc ctcgattatt gctcagacgc ggttaaaaaa tccggaagaa
     2161 ttcggcaaag tgaccctgcg cgtaaacagt gacggctcgg tggtgcgtct gaaagatgtc
     2221 gcacgggttg aacttggcgg tgaaaactat aacgttatcg ctcgcatcaa cggaaaaccg
     2281 gcggcgggcc tgggtattaa gctggcaacc ggcgcgaatg ctctcgatac cgcgaaagcc
     2341 attaaggcaa aactggcgga attacagcca ttcttcccgc agggaatgaa ggttctctac
     2401 ccttatgaca ccacgccatt cgtccagctt tctattcacg aagtggtaaa aacactgttc
     2461 gaagctatta tgctggtgtt cctggtgatg tatctgttct tgcagaatat gcgagcaacg
     2521 ctgatcccca ccattgcggt acccgtggtg ttgttaggga cgtttgccat cctcgccgct
     2581 tttggttact ccatcaacac actaacgatg ttcgggatgg tgcttgccat cgggctgctc
     2641 gtcgatgatg cgatagtggt ggtggagaac gtcgagcgcg tgatgatgga ggataagctc
     2701 ccgccaaaag aagcgacgga aaaatcaatg tcgcaaatac agggcgcact ggtgggtatc
     2761 gcgatggtgc tgtcagcggt atttattccg atggcattct tcggcggttc tactggggca
     2821 atttatcgtc agttctctat caccatcgtt tcggcaatgg cgctttctgt tctggtggca
     2881 ttgattctta cccctgcgtt atgtgcaaca ctgctcaaac ccacctcagc tgagcatcac
     2941 gaaaataagg gtggtttctt cggctggttt aataccacct tcgatcatag cgttaaccac
     3001 tacaccaaca gcgtcggcaa aatcctcggt tccacaggac gatatttact gatctatgcg
     3061 ctgattgttg caggaatggt ggtgttgttt ttacgtcttc cgtcttcctt cttacctgaa
     3121 gaggatcagg gtgtctttct gaccatgatt cagttacccg ctggcgcgac gcaagagcgg
     3181 acgcaaaaag tgttggatca agtgacggat tactatctga agaacgagaa agcaaacgtt
     3241 gaaagtgtct ttacggttaa cggctttagc ttcagcggcc aggcacctcc gcaaccccat
     3301 gttttcacat aactgttgcg ttgaccaatt gaatctacag gtatggcctt cgtcagtctg
     3361 aaaccgtggg aagagcgtaa tgctgatgaa aacagtgcgg aagcggtaat ccatcgtgcc
     3421 aaaatggagt tgggcaagat ccgcgacggt tttgtcattc cattcaatat gccagccatt
     3481 gttgaactgg gcacggcaac gggtttcgac tttgagttaa ttgataaggc tgggctgggt
     3541 cacgatgccc taacccaggc ccgtaaccag ttgcttggta tggcggcgca acatcctgcc
     3601 agcttagtca gcgtgcgtcc caatggcctg gaagacaccg cgcagtttaa actggaagtt
     3661 gaccaggaaa aggcgcaggc attaggtgtt tcactttctg acatcaatca gaccatttca
     3721 acggcgttgg gtgggactta cgttaacgac ttcatcgacc gtggtcgcgt gaaaaaggtg
     3781 tatgttcagg cggatgccaa attccgtatg ctgccagaag atgtcgataa actttatgtc
     3841 cgcagcacca acggcgaaat ggtgccattc tctgccttta ccacttcaca ttgggtgtat
     3901 ggctctccgc gactggaacg ctacaacggt ctgccgtcaa tggagattca gggggaagcc
     3961 gcgccaggaa ccagttccgg cgatgccatg gcgttgatgg aaaacctagc gtcaaaatta
     4021 cctgcgggca ttggttatga ctggacgggt atgtcgtatc aggaacgctt atcgggaaac
     4081 caggctcccg ctctggtagc aatttccttt gtggttgttt tcctgtgcct tgctgcactc
     4141 tatgaaagct ggtcaattcc tgtctcggtt atgttggtcg tgccgttagg gattgtcggc
     4201 gtgctgctgg cggcgacact ctttaatcaa aaaaatgacg tctactttat ggtaggcttg
     4261 ctaacgacaa ttggcttgtc ggccaaaaac gctattttga ttgttgagtt cgctaaagat
     4321 ctcatggaga aagagggtaa aggtgttgtt gaagcgacac taatggcagt acgtatgcgt
     4381 ctgcgtccta tcctgatgac ctctctcgcc tttattctcg gcgtattacc gctggctatc
     4441 agtaacggtg ccggcagtgg cgcgcagaac gcagtgggta tcggggtaat gggaggaatg
     4501 gtctctgcaa cgttgctggc aatcttcttc gtaccggtgt tctttgtggt gatccgccgt
     4561 tgctttaaag gataaatctg aaacataaag gcgctttcgg gtgcctttat gttttccagt
     4621 gaaacccata aaaattaata agatattctt ttgctcactc tttaaaagct ttctatagct
     4681 cccgctccct tcactatttt tacaattcac ataatttgtc attattcatt ccgcaagatt
     4741 tataacctcc ggtgataaaa tggcattcag ctcgttaata agagagttaa cttattaagc
     4801 gttagcgttt attactgagg taacaccatg aaaagattaa ttcctgtggc attgctcacc
     4861 gcattgctgg cgggctgtgc tcacgattcg ccctgcgttc cggtttatga cgatcagggt
     4921 cgtctggttc acaccaatac ctgtatgaaa ggcacgactc aggataactg ggaaacggct
     4981 ggggctattg ctggcggggc agcggctgtt gctggcctga cgatggggat catcgctctg
     5041 tcgaaataac tgaacagggc gcggagtttc gcgctctgtt taacgataca gtatctttac
     5101 ataagataaa atattgctat attctgctgg ctaattctct gctgatttag catgttatat
     5161 cgtgttattt cgctgctttc gaaataacgc atcaatttaa ctttcgcact tcccaccaga
     5221 aatatactca cgctaacaat cattattctg cttagtaaaa cacgcgtaaa tgttttgcgc
     5281 caaaatatgg cgcatgtttc attttcgcac cattgcgggg cgctgttttt attatttcct
     5341 gtctacactc agcttattgc gacgcgaaat cgtgcgcaaa agctggcact acttttgctt
     5401 ataagaggat ggccacagac aggcaaaaga cgtttcccaa acgtcctata acgataattt
     5461 tcgcctcaca ggaagcatta tgaaaaagat gatgatagcc acactggctg ccgccagcgt
     5521 gctgcttgcc gttgcaaatc aggcgcatgc tggcgcgacg cttgatgccg ttcagaaaaa
     5581 aggttttgtg caatgcggga tcagtgatgg attacctggg ttctcttatg ccgatgcaga
     5641 cggtaagttt tcaggtattg atgttgatgt ttgtcgtggt gttgccgctg ctgtatttgg
     5701 tgacgacacg aaagtgaaat ataccccact cacagcaaaa gaacgcttca cggctttaca
     5761 gtcaggggaa gtggatttgc tctcccgtaa tacgacctgg acttcatctc gcgatgccgg
     5821 gatgggaatg gcatttactg gcgtcactta ttacgacggc attggcttcc tgacgcacga
     5881 taaagcgggg ctaaaaagcg cgaaagaact ggatggcgct accgtctgta ttcaggcggg
     5941 tactgatacc gaactcaacg tcgccgacta tttcaaggca aacaatatga agtacacacc
     6001 agtgactttc gatcgttctg acgaatcagc gaaggcactg gaatccggtc gctgcgatac
     6061 gctggcttcg gatcaatccc aactgtatgc cctgcgcatc aaattaagca acccggctga
     6121 atggattgtc ttaccggagg ttatctctaa agaaccgctt gggccggtag ttcgtcgtgg
     6181 cgatgatgaa tggttctcga ttgtacgctg gacgcttttc gccatgctaa atgctgaaga
     6241 gatgggcatc aattcccaga acgtcgatga aaaagcggct aatccagcaa cgcctgatat
     6301 ggcacatctg ctgggtaaag aaggcgatta cggcaaggat ctgaagttgg ataataaatg
     6361 ggcctataac atcatcaaac aggtgggtaa ctactcggaa attttcgagc gtaacgtagg
     6421 ttcagaaagc ccgctgaaaa ttaaacgtgg gcaaaataat ctctggaata acggcggtat
     6481 tcagtacgca ccgcccgtgc gttaagtcgg tgtgaggtaa cgagcgtcgc tacggcggcg
     6541 ctctcttcag cgttatgctt tccgaggttt ctatgtctca tcgccgctca accgttaaag
     6601 gctcactctc ttttgccaac cctacggttc gcgcctggtt attccagatc cttgccgttg
     6661 ttgctgttgt cggcattgtt ggttggttat ttcacaacac tgtaacgaat ctcaataatc
     6721 gtggcattac ttcaggtttt gcctttctgg atcgcggcgc tggcttcggt attgtccagc
     6781 atttgatcga t
//
LOCUS       DAZGXS010000190         6640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGXS010000190 DAZGXS010000000
VERSION     DAZGXS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(387..659)
                     /locus_tag="ACHKG5_004415"
     CDS             complement(387..659)
                     /locus_tag="ACHKG5_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0870390.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(665..1216)
                     /locus_tag="ACHKG5_004416"
     CDS             complement(665..1216)
                     /locus_tag="ACHKG5_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP0870391.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1213..1965)
                     /locus_tag="ACHKG5_004417"
     CDS             complement(1213..1965)
                     /locus_tag="ACHKG5_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFP0870392.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2899..3159
                     /locus_tag="ACHKG5_004418"
     CDS             2899..3159
                     /locus_tag="ACHKG5_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870393.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3156..3713
                     /locus_tag="ACHKG5_004419"
     CDS             3156..3713
                     /locus_tag="ACHKG5_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0870394.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTQPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3710..3931
                     /locus_tag="ACHKG5_004420"
     CDS             3710..3931
                     /locus_tag="ACHKG5_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870395.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3931..4254
                     /locus_tag="ACHKG5_004421"
     CDS             3931..4254
                     /locus_tag="ACHKG5_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFP0870396.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4268..6600
                     /locus_tag="ACHKG5_004422"
                     /pseudo
     CDS             4268..6600
                     /locus_tag="ACHKG5_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
BASE COUNT         1542 a         1620 c         1701 g         1777 t
ORIGIN      
        1 ttggggtgct cgaaattata tttcgtttcg agcgctcagt tcatttctaa ataatttgca
       61 aaattatttt gggtactttc gcctctacac ttccttcggg tggttccaca ataatggatc
      121 tcaccggcgt ttgatatttt ataatctaac cccttagcgc gcgcagcccc ccacgcctgc
      181 tcgcttcgct taacagactg gttttcatgc attccgcaaa tcgtctcaga agccaccaca
      241 caagggcttt cgcgtcaaaa atggtgcatg agaatcatgc gttttcatgc gctatagaca
      301 tgcactcatg cgctctcagg ccagccaggg aaaaggcgta aaaaatccca gtactggacc
      361 gggattacgt gggctttttt tgctaatcag acaggaattt gctgacggct tgacagcttt
      421 gacgcggagc catagcgatt gagagtttgc tgttgttttt ccggcatttg tgctttctct
      481 gattcctgtt cattgcgccg gggcttcatg attatcgtat ccacactttc cagtgcggta
      541 aatgtgtagg aacattcaag attctggcac tgataccatg agcgtttaac cgatggtgct
      601 tcataggcgg aggttctggc gtgtgccgtc gtaccgcatt cgggacattt aagtgccatt
      661 aatattactc ctgcatgccg ttaatgtaat ccagacgttg ctgaaagcgc agttgttgtg
      721 caggggtgta agagctctgg caatcacgaa ccatcatggg gtcaggcatc acaccggatt
      781 catccagaat catacgataa tccccggcaa tttcaggcgg tgttattgcc agctggcgaa
      841 ccagagcatt gcgtaatata cgggacgcaa cgtcaacccc tccgcgcccc ttcaggaacg
      901 gagccagtgc agatgtcagg gctgctccat tcgcctgcat aaaattgctg agagcgtctt
      961 ctgtgcaggc ctccatcagt tgatgctgcg agtaaatgca ttcgcgcgca gcacagttaa
     1021 tttgccattt cagcacatcc agatgttccc gctgtaattc gagctgtcga gcataatctg
     1081 ctggtttttc catggcagtg aggagtgatt ctatctgttg ttcaacggct tttcggctct
     1141 cactatgtcc agcccacaga atacgggcat ttctaaaact gataagcgct tggtctggag
     1201 tgactcgggc catcatactg ctttctcctt ctcagcacgt tcctgacgaa ccttcatttt
     1261 atgcatagct gctggagaga gtttattact gaaatccaga cgggccgcat aatccggggc
     1321 aactcccgcc agtttaaaca cggggtcctg ttcgggcatc gcatcgttag tcagagccgg
     1381 tctggtgata tgtttatgaa taaaatcttt cagcagtgtg tccgggtcat taactgaatg
     1441 caccacaccg acaactgcgc tggcttccct gcccattgtt gatttgagca ggcttagtgt
     1501 ctggattagt gcttttccat gcgactgcat aaaatcttcc cagatttgtt ttgcgcgaat
     1561 acccacaagg gtgtcgtgcg cgtggatata ctttccggct aattccgcag cctcctgcgg
     1621 caaaagtgca ttttcgcttt cctgagccgc cagcagctca tcaaaatcct caagcgtttc
     1681 tcgtcccagc gcaatttccg tgcgcagttt tttcatttcc tttgagacta cgccctgact
     1741 ttcacgaaat agcgtgcgcc actcctcatt cagggcgttc gtcgtggctt ccatttcagc
     1801 gcggcgctgg cggattgtcg caatattatc cgctgccgct ttttgctgac gacgtgcttc
     1861 aagccatgca gatttggtgg cattcacttt ctcaagcgcc tgttgcgtcg aaacagggat
     1921 agaaatttca acgatcgggt tattgttctg ttctgggatt gtcatgtcag cacctagtgg
     1981 tttgtcgtgg tgtcaattgt gctgtgatgc atacaaagct gccattgaac gccattgtgc
     2041 gagcgacaag acaaagactg aaccgccccg gacctctttt ggctagccag aaaaggcctc
     2101 gtttaactaa ggctgtttca gacttacctt aactattcac tactattcac ttaggtaaaa
     2161 aaaataggta atacagaagg ttataaggtg aagggttggc atggcagtat tcatcgactg
     2221 ttcataactg ttcacatggg aataaaatta tttatggtat tttttcataa tgggtggttt
     2281 ttttattcct cgtagctatt tgttttttgc tcctcaccac tattcaccct tattcaggag
     2341 tattcaacag gcacataaat agactgcatg tttttgccgg ggaataacat tttcgcctct
     2401 ggccatggtg ttgcatttca tactgatttt tataaaacta gatctgtccg gttctttatg
     2461 gattcattcg gatatatttt acgatttaag gtgatgttat ttaatatcaa ggtttttatg
     2521 caggcccaat gtttataagt tggatgcgat tcaaaaatat gcccggaaat tggggcctgc
     2581 aatgcaaggc ctcgtgtaga agttgtactg ccttgctgta ttggcggtgg ggttagcact
     2641 atcaggtgca atgacccata ggatagtaaa ccctgggtga atggtagttc ctattaatgc
     2701 tcctgtattt atagtccgca ggcatggtat ttattatgtc attttatatg gtttaatcgg
     2761 gctttaaatt ccataaaatc aatatgtgac gctaaataaa aaatgcgtgc gcaggtggtt
     2821 tatatacaca taataaggaa ctacctgaac ccggataaat tcatccggac tgttgtggaa
     2881 attaaagagg gtagataaat gcgtaatatt tctgtgtctg cgccagctcc tgctgcgcca
     2941 ttatttcctg tatcgaacca tcatgagcga tttttacgcc tgcctgaggt gatgcactta
     3001 tgcgggttgt cccgttcgac cgtttacgac ctgatcagcc gcgatgcgtt tccgaagcag
     3061 atcccgcttg gcggtaaaaa tgtcgcctgg gcacagtctg aggtcagcgc atggatggcg
     3121 gaccgcatca gcgcccgtgg gcggggatgt gatgcatgat gatacctgca tacattaaat
     3181 acctttattc tggcttgctt actgtcgtca tttccaggta tagtttttcc gctgtcgcaa
     3241 aatcggcagc cggaattggc gttccgtata acttattggc gacaattgac gccccttgcg
     3301 tcttttttta cgtcgtagct caggcacaac cattttcagg gctgtggtgt ttatgcttac
     3361 accatggttc tatcgagata atggtagtcc gggcggggca gccttcgggc tggccggttt
     3421 ccaataaggc cggttacgcc aaccccgttc gggctgccac cagtgaaatt ggcgtttccg
     3481 gtggtagcaa taaccgctac ttattggagg ctgccatcat ggctacaatc ctcaccccgt
     3541 cacacccgca atatgtcttt gtgtttgccg caatccgccg tgctgacact caaccccgca
     3601 tctgtatgct tcgcactgtc tcctgcgatg agcgttccgc gcgccgtttg ctagtccgag
     3661 attatgtact ctccctttct gcacggctgc ctgccgggga ggtgacgcta tgaaccagtt
     3721 tgagatttcc tacgacgatg ttgtaaggct gaaacattta cgcaatgtgg gtgagtatgt
     3781 aactggcatg gcagccctgc aggactgtta tgaaaagcca gcaggtgccc aatgcgaaca
     3841 actggtttcc ctcatttatc tgatgacaga gcaactggat ggagtggtgc aacgctgcca
     3901 tgacgacctg atgaatgcgg aggtggcctg atgatatgct gtgaatctct tctggcactt
     3961 cgtgccgctc tgtatcgccg tgccgttgcc tgtgcctggc tggctctaag caatcatcag
     4021 gaacgttatt ccggcctgac gctcgctgaa cttgaagatg ccatagcccg cgagctggaa
     4081 gggttctatc tgcgccagca tgggcagcaa agagggctgg aaattgcctg cgcgttgctt
     4141 tcggatctga tggagtcggg gccgcttaaa gcctgtccgg ttctctcact gctcggaatg
     4201 acggtcatgg atgaactttg ttcccgtcac ctcaacaaac cagcgctgca ctaaggaggg
     4261 ccgcacaatg tcaggaatga aagttagcca ggctgagaaa gcagctcggg gtcactggtc
     4321 aagaatttta cctgcgctgg gcgtaaatgt actgaaaaat cggcaccagc cctgcccggt
     4381 ctgtgccggg aaagaccgct ttcgatttga tgaccaggaa gggcgggaac gtggttctgt
     4441 aaccagtgcg gggcaggtga tggcctggcg cttgtaagta aagtactgga tgtaggcatt
     4501 agtgaagcgg cagacagaat aaacggcatt atcggaaacc tgctgccagt atctcaggga
     4561 atgcttgaat ctggttctcc tgaaaaagag gacgggagaa aagctgcagc agtgctggct
     4621 gcccgtttgt ttgataagtc ccgccagacc actggcaatg cctatctgac gagtaaaggg
     4681 tttcctgcac tgccttgccg ggaattaacc gctatgcata aagtcggtgg tgtggcattt
     4741 cgcgcgggag atcttgtcgt tccattgtat gcagatggag agctggtaaa tctgcagtta
     4801 atcaacgcta atgggggcaa atgcttcctt aaaggcggtc aggttaagaa tgccttttac
     4861 ctggttgaag gtactgccaa agcagccaaa cggctctgga tagcggaagg atatgccacc
     4921 gcacttacta tcaactatct gactggcgat gctgtcatgg tggccttttc gtccgtcaat
     4981 ttcctttccc tggcgagcat tgcctgcagt gagtacccaa cgcaccagat aattattgct
     5041 gctgaccgcg atctcaacgg tgcggggcaa acaaggggcg cagctgttac cggggcctgc
     5101 aattgcacaa tggcgctccc gcctgtgttt ggtgactgga acgatgcatt cacgcaaaac
     5161 ggcgaagaag ccacccggca tgcaattcat gaagtaataa aaccagctgt tgccagcccc
     5221 ttcgacacaa tgagcgaagc tgaatttacc gcgctgagcg tcagcgaaaa agcgcagagg
     5281 gtagtggatc actataaaaa ttcactggca gtagacccga acgggcagct cctttcacgc
     5341 tatgaggcgg gggcctggaa agttatctat tacgccgatt ttgcccgtga tgtcgctgcg
     5401 ctgtttcagc gcctcgacgc acctttttca tccgcgaaaa ttgcgtctct cgtggaaacc
     5461 ctcaaactga tcgttccgca acagcagaat ccggcgcggc aacttatcgg atttcgcaac
     5521 ggtgtgctcg atacccggac aggattgttc agcccgcacg ataagaagca ctggttacgt
     5581 acgctgtgcg aggtggatta cacgcagccc gttgacggtg agtcactgga aacccatgcc
     5641 ccggcattct ggcgctggct ggatcgtgcc gcaggtttta atcctgaaaa acgggacatt
     5701 attctggctg cattgtttat ggtgctggct aaccgttatg actggcagct gtttctggag
     5761 gtcactggcc ctggcggaag tggaaagagt attcttgctg aaatagcaac catgctggcg
     5821 ggtgaagata acgcgacctc ggcaaccatt gaaatgcttg agtcgccaag agaacgagct
     5881 gcgttaatag gtttttcact gattcgactt cccgaccagg aaaagtggag cggtgacggg
     5941 gccggactaa aagccatcac tggcggcgat gcggtatccg ttgatcccaa atatcagaac
     6001 gcctattcaa cccacatccc ggcggtcatc ctggctgtga acaataatcc gatgcgcttc
     6061 actgatcgta gtggtggagt ttcacgccga agggtgatcc tgcatttccc cgaccagata
     6121 gccccggagg aacgcgatac ccagctcaaa gagaaaattg ccagtgagct agcggtgatt
     6181 gttcgccagc ttatgcagcg tttcagcgac ccaatgagtg ccaggacatt gcttcagtca
     6241 cagcagaact ccgatgaagc gctcaccatc aaacgtgatg ctgattcagc ttttgatttt
     6301 tgcggctacc ttgaggtcct acctgacacc acgggcatgt ttatggggaa cgctaatatt
     6361 gttccacgtc agcctcgaac ttacctctac catgcctatc tggtctacat ggaggctaac
     6421 ggctataaaa atacgctcag tctgaccatg tttggcaagg ggctaccgtt aatgctcaag
     6481 gaatatgggc tgcagtatga gaaacgacgg accaatcaag gaatgcagac taatctggcc
     6541 ctaagagagg aaagcaatgc tgactggttg ccaaaatgcg atgagtttgc agcgaaataa
     6601 ctgacctaag accggctgtg ccggtcttaa atccgctaca
//
LOCUS       DAZGXS010000191         6627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGXS010000191 DAZGXS010000000
VERSION     DAZGXS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..379)
                     /locus_tag="ACHKG5_004423"
     CDS             complement(<1..379)
                     /locus_tag="ACHKG5_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870397.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHKG5_004424"
     CDS             complement(436..831)
                     /locus_tag="ACHKG5_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0870398.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHKG5_004425"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHKG5_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHKG5_004426"
     CDS             complement(1642..2382)
                     /locus_tag="ACHKG5_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0870399.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHKG5_004427"
     CDS             complement(2389..3351)
                     /locus_tag="ACHKG5_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870400.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHKG5_004428"
     CDS             complement(3374..3799)
                     /locus_tag="ACHKG5_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0870401.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHKG5_004429"
     CDS             complement(3796..4098)
                     /locus_tag="ACHKG5_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0870402.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHKG5_004430"
     CDS             4196..4567
                     /locus_tag="ACHKG5_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870403.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHKG5_004431"
     CDS             4588..4779
                     /locus_tag="ACHKG5_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP0870404.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHKG5_004432"
     CDS             4781..5059
                     /locus_tag="ACHKG5_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0870405.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            5412..5711
                     /locus_tag="ACHKG5_004433"
     CDS             5412..5711
                     /locus_tag="ACHKG5_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870406.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            5783..6001
                     /gene="ydfC"
                     /locus_tag="ACHKG5_004434"
     CDS             5783..6001
                     /gene="ydfC"
                     /locus_tag="ACHKG5_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP0870407.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            6465..>6627
                     /gene="dicB"
                     /locus_tag="ACHKG5_004435"
     CDS             6465..>6627
                     /gene="dicB"
                     /locus_tag="ACHKG5_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0870408.1"
                     /translation="MLWGDSLYPALIVNWLTSPGGTRHRITKFIVEDAIMETLLPNVN
                     TSEGCFEIGV"
BASE COUNT         1638 a         1772 c         1524 g         1693 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg
     5161 tttattaaac taaaaatacc aacccacccc gccccacaga acgcagggaa atacttcgag
     5221 ttacccggca gtggtcaggg gttaagtagc cagcccgagg cgtatgaaca tgacggcagg
     5281 gttcaacttt aataactatg cagcaggttt ttgttccgct accccggcgt taaggggaaa
     5341 cagagggttt ctcagtgggc gaagtcaaac atcagaatgg aaggcatccc gggatcggca
     5401 aagaagcagc aatggcgctt tatattgaca tcagcgccat tgcaggacag gtaagagtta
     5461 tcagagcggt aactaagcgg tatgcgcctt tacttcagaa agtctctggt gagtgcaccg
     5521 aagatattgt caacgatttc gtcatcgaac tgcgaggact catcttcagt tacaaggtga
     5581 ccacaatttt tgcagatggc tcccgcgaaa ctgtcagagc cctgcggctt aaaggatgtg
     5641 tcaaagactt cgccaccaca ttctgggcaa gaaaacttga ttgtattcat aaccaatttc
     5701 ctctcgagta acagacccct cagaggatac cacctcgcct gacgtggtta aaagcaggca
     5761 acgctaacca caaggagccg acatgcagaa acgagaaccc gtcatcatcg cgccagacta
     5821 taccgatgat gaactttatg agtggatgca ccagaaaatt aatgcagcgc aggatctgaa
     5881 atgggccaat gaagccaggg ctaagcaggc tgaaaatctg tccgctctgg agcaggatat
     5941 caccaatctg gaaaaagcag cggcattaag cattgccaga atgattacat acccgcgtta
     6001 atagctaacc aacgaagcta aggttggtaa ttaaggagtt ctccacgggt gaggtggagt
     6061 gcgtgcgccg gacacgggtg agcatacggc actgacagtt tactgaaagg atatttccct
     6121 gaaaagtcag accataacgc gaaagcgcac ggcgaggtag ctggttcata gatagcctgt
     6181 cgttaaattt tcgtcgaccg tgcgcttccg gttgtggcaa tccgcgaaat ggcgcggcgg
     6241 taagtatgga ggggttattc cttcccccgt tgaggacacc gggttgtcag gttgaccata
     6301 cgcttaagtg acaaccccgc tgcaacgccc tctgttatca attttctggt gacgtttggc
     6361 ggtatcagtt ttactccgtg actgctctgc cgcccttttt aaagtgaatt ttgtgatgcg
     6421 gtgaatgcgg ctgagcgcac gcggaacagt taaaaccaaa aacagtgtta tggggggatt
     6481 ctctgtatcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc aggcaccgca
     6541 tcacaaaatt cattgttgag gacgcgataa tggaaacgtt attaccaaac gttaatacgt
     6601 ctgaaggttg ttttgaaatt ggtgtca
//
LOCUS       DAZGXS010000192         6621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGXS010000192 DAZGXS010000000
VERSION     DAZGXS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            125..328
                     /locus_tag="ACHKG5_004436"
     CDS             125..328
                     /locus_tag="ACHKG5_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0870409.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHKG5_004437"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHKG5_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHKG5_004438"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHKG5_004438"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKG5_004439"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKG5_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0870410.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHKG5_004440"
     tRNA            2484..2559
                     /locus_tag="ACHKG5_004440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..>6621
                     /locus_tag="ACHKG5_004441"
     CDS             2873..>6621
                     /locus_tag="ACHKG5_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFP0870411.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVL"
BASE COUNT         1872 a         1565 c         1611 g         1573 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc a
//
LOCUS       DAZGXS010000193         6513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGXS010000193 DAZGXS010000000
VERSION     DAZGXS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(58..2091)
                     /gene="betT"
                     /locus_tag="ACHKG5_004442"
     CDS             complement(58..2091)
                     /gene="betT"
                     /locus_tag="ACHKG5_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0870412.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2220..2807
                     /gene="betI"
                     /locus_tag="ACHKG5_004443"
     CDS             2220..2807
                     /gene="betI"
                     /locus_tag="ACHKG5_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0870413.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2821..4293
                     /gene="betB"
                     /locus_tag="ACHKG5_004444"
     CDS             2821..4293
                     /gene="betB"
                     /locus_tag="ACHKG5_004444"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0870414.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4307..5995
                     /gene="betA"
                     /locus_tag="ACHKG5_004445"
     CDS             4307..5995
                     /gene="betA"
                     /locus_tag="ACHKG5_004445"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0870415.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1602 a         1796 c         1772 g         1343 t
ORIGIN      
        1 gcgagtcaat gattcccgcg ccatatgcag acatggcgcg gttttatgca ataacaatca
       61 cgcgtccggg aacatcacgc tatggcccgg cgcttcacga tggagatgaa taaagttaag
      121 gtgccgctcg tactggtcaa gaatatcggt gatcacctgc tctttgctgt agtccatcag
      181 gtcgttgccc tggctgcctt ctaacaggaa ggtttccagc cggtagtagg tcgatttccc
      241 gctacgtgcg cggtaggtaa agcccggcac cgagtattgc tgcggccaaa tctgatagac
      301 aaagttttgc tcttcgccca tatgcaccaa caaatccaga tgccccaact gttgtccctc
      361 ttccggcggc aggcttttta gctccacgta cgcgccgcgc aaccgcaact cctgcgccac
      421 ttcttccatt gccgggtaac agaccgtctc catcatctgt ttagtgtaac gcgtgcccgg
      481 ataattcatc aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa gccccagcgg
      541 tcgcggtgcg gtatcgcggt tggcactttc acggcggtaa tcttctacct tcagagattt
      601 atacaacccc gccatcacga agaagatcac aaagctgaac ggcagcccca taatcaccgt
      661 ggtgttttgc agcgcggata tcccgttggt catcagcatg ccgagcgtca gcaggccaat
      721 cgccaccgac cagaagacgc gcagccagcc gggggcgtcg ctgttgatat ctttaagctg
      781 cgaggtgaaa ttccccagca ccagcgcccc ggagtccgcc gaggtcacat aaaacagcag
      841 gccagtaatg gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg ccagcaggct
      901 gtagaagccg cgctccggat ggaccatcgc ttcctcggca aatgccgcgc cgccgtggat
      961 gatttcatac agcgcgctat tgccgaacac cgagagccat aacagcgtga aggtaaacgg
     1021 aataatcaac gtgcccagca cgaactggcg aatggtacgc ccacgcgaga tacgcgccag
     1081 gaacaagccg acaaacggcg accatgccac ccaccatgcc cagaagaaga gcgtccagtt
     1141 attcatccac tcaaccgggc ggtcgaaggc aaaactgttg agcgtcatgc ccataaagcg
     1201 attcacatag tcgccaacat tcagcaccag tgcattaagc aggaacgaag tgtcgcccat
     1261 aaacaacacg aacaggatca atcccagcgc cagcgcgaca ttaagctccg ataacacgcg
     1321 aatgccctta tcgacaccgg atgtcaccga gatagtggcg attatcaccg acaaggcgat
     1381 cagtgccgct tttgccgcca tcgaatcggg aatatcaaac agtacgctca agccatagtt
     1441 aagctgcacc acaccgatac cgagcgtagt ggcaataccg aagatagtgc cgatcaccgc
     1501 tgcaatatcc actgagtgac ctatcggccc gttaatccgt ttaccgaaga tcgggtacag
     1561 cgccgagcgg atggtgagcg gcaaattata acgatagcta aagtatccga gcgccatgcc
     1621 catcagcgca tacatcgacc agccggttaa gccgtagtga aacagcgtcc agaccatcgc
     1681 ctgacgcgcg gcctcaatcg tctgtcccgc gccttccggc ggctgcatat actgcgttac
     1741 cggttcggct acggaaaaga acatcagatc gataccgatc ccggcagcaa acagcatcgc
     1801 cgcccaactc agcaggctga attccggttt ggattgttct ggcccgagct tcaccgaacc
     1861 aaaacgcgaa caagcgatac agaccacaaa gacaatatag agcgttgccg ccagcagata
     1921 gtaccaaccg aaggttttag aaacccagtc cagcgtgcgg ccaatccaca gggccgagaa
     1981 gtcgcgaaac aggatcgttg tcagggaaaa caacaaaatc agtccggcgg aggtgtaaaa
     2041 caccaccgga ttgattttgt ccttttccct gctgtgtgaa aggtctgtca tccagtatcc
     2101 ccactgttat tgttaccatt taaaatcaaa ttcgtaacaa ttaagacaca ttttatattg
     2161 aacgtccaat caataaccgc tttaatagat aaacaacgct gatgaatgga gtggcaaaaa
     2221 tgcccaaatt ggggatgcag tcgatccggc gcagacaact gatcgacgcc acactggaag
     2281 caataaatga agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc cgtgcaggcg
     2341 tttctacggg gatcatcagc cactatttca gggacaaaaa tggtctgctg gaagcaacca
     2401 tgcgcgatat caccagtcag ctgcgtgacg cggttttgaa tcgattacat gcacttccgc
     2461 agggcagtgc agagcagcga ttacaggcga ttgttggcgg aaacttcgat gaaacgcagg
     2521 tgagcagtgc ggcgatgaaa gcctggctgg cgttctgggc cagcagtatg catcagccga
     2581 tgctctatcg tttacagcag gtcagcagcc gccgcttgct gtcgaatctg gtgagcgagt
     2641 ttcgccgcga attgccgcgc caccaggcac aggaagcggg ctacggcctt gccgcgctga
     2701 ttgacggatt atggctgcgc gcggcactga gcggcaaacc gctggataaa cctctcgcac
     2761 actcgctgac ccgtcacttt attactcagc atttacccac cgattaaccg aggagacgtg
     2821 atgtcccgaa tggcagaaca gcagctttat atacatggtg gttatacctc cgccaccagc
     2881 ggtcgcacct tcgagaccat taacccggcc aacggtaacg tgctggcgac cgtgcaggcc
     2941 gccgggcgcg aggatgtcga tcgcgccgtg aagagtgccc aacaggggca aaaaatctgg
     3001 gcggcgatga ccgctatgga acgctcgcgt attctgcgtc gggccgtcga tattctgcgc
     3061 gagcgcaatg atgaactcgc caaactggaa accctcgaca ccggaaaagc gtattcggaa
     3121 acctcaaccg tcgatatcgt taccggtgcg gacgtgctgg agtactacgc cgggctgatc
     3181 ccgtcgctgg aaggcagcca gatcccgttg cgtgagacat catttgtcta cactcgccgc
     3241 gaaccgctgg gcgtggtggc agggattggc gcatggaact acccgatcca gattgccctg
     3301 tggaaatccg ccccggcgct ggcggcgggt aatgcgatga ttttcaaacc gagcgaagtc
     3361 actccgctta ccgcgttaaa gctggctgaa atttacagcg aagcgggcct gccggacggt
     3421 gtatttaacg tgttgccggg cgtgggcgcg gagaccgggc aatatctgac cgagcatccg
     3481 ggcattgcca aagtgtcgtt taccggcggc gtcgccagcg gcaaaaaagt gatggctaac
     3541 tcggcagcct cttccctgaa agaagtgacc atggaactgg gcggtaaatc accgctgatc
     3601 gttttcgacg atgcggatct cgatctcgcc gccgatatcg ccatgatggc aaacttcttc
     3661 agctccggtc aggtgtgtac caatggcacc cgcgtcttcg ttccggcgaa atgcaacgcc
     3721 gcatttgagc agaaaattct ggcgcgcgtt gagcgcattc gcgcgggcga cgttttcgat
     3781 ccgcaaacta acttcggccc gctggtcagc ttcccgcatc gcgataacgt gctgcgctat
     3841 atcgccaaag gcaaagagga aggcgcgcgc gtactgtgcg gcggcgatgt actgaaaggc
     3901 gatggcctcg ataacggcgc atgggttgca ccgaccgtgt tcaccgattg cagcgacgag
     3961 atgaccatcg tgcgtgaaga gatcttcggg ccggtgatgt ccattctgac ctacgagtcg
     4021 gaagacgaag tcattcgccg cgccaatgat accgactacg gcctggcggc gggtatcgtg
     4081 acggcggacc tgaaccgcgc gcatcgcgtc attcatcagc tggaagcggg tatttgctgg
     4141 atcaacacct ggggcgaatc cccggcagag atgcccgttg gcggctacaa acactccggt
     4201 attggccgcg agaacggcgt gatgacgctc cagagttaca cccaggtgaa gtccatccag
     4261 gttgagatgg ctaaattcca gtccatattc taaccaggag gtttatttgc aatttgacta
     4321 catcattatt ggtgccggct cagccggcaa cgttctcgct acccgtctga ctgaagatcc
     4381 gaatacctcc gtgctgctgc ttgaagcggg cggcccggac tatcgctttg acttccgcac
     4441 ccagatgccc gctgccctgg cattcccgct acagggtaaa cgctacaact gggcttacga
     4501 gacggaacct gaaccgttta tgaataaccg tcgcatggag tgcggacgcg gtaaaggcct
     4561 gggtggatcg tcgctgatca acggcatgtg ctatatccgt ggcaacgcga tggatctcga
     4621 caactgggca aaagaacccg gtctggagaa ctggagttat ctcgattgcc tgccctacta
     4681 ccgcaaggcc gagacccgcg acgtgggcga gaacgactac cacggcggcg acggcccggt
     4741 gagcgtcacc acctccaaac ccggcgtcaa tccgctgttt gaagcgatga ttgaagcggg
     4801 catgcaggcg ggctacccgc gcacggacga tctcaacggt tatcagcagg aaggtttcgg
     4861 cccgatggat cgcaccgtca cgccgcaggg ccgccgcgcc agcaccgcgc gcggttatct
     4921 cgatcaggcc aaatcgcgcc caaacctaac cattcgtact cacgccatga ccgatcacat
     4981 tatttttgac tgtaaacgcg cggtgggcgt cgagtggctg gaaggcgaca gcaccattcc
     5041 gacccgcgcg acggcgaaca aagaagtgct gttatgtgca ggcgcgattg cctcaccgca
     5101 gatcctgcaa cgctctggcg tcggcaacgc tgaactgttg gccgagtttg atattccgct
     5161 ggtgcatgat ttacccggcg tcggtgaaaa tcttcaggat catctggaga tgtatctgca
     5221 atatgagtgc aaagaaccgg tttccctcta ccctgccctg cagtggtgga atcagccgaa
     5281 aatcggtgcg gagtggctgt ttggcggcac cggcgttggt gccagcaacc actttgaagc
     5341 aggcggattt attcgcagcc gagaggaatt tgcgtggccg aatattcagt atcacttcct
     5401 gccggtagcg attaactata acggctcgaa tgcagtgaaa gagcacggct tccagtgcca
     5461 cgtcggctcg atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc gcgacccgca
     5521 ccagcatccg gcaattctgt ttaactacat gtcgcacgaa caggactggc aggaattccg
     5581 cgacgcaatt cgcatcaccc gcgagatcat gcatcaaccc gcgctggatc agtatcgtgg
     5641 ccgcgaaatc agccccggca cggaatgtca gacggatgag cagctcgatg agtttgtgcg
     5701 taaccacgcc gaaaccgcct tccatccgtg cggtacctgc aaaatgggct acgacgagat
     5761 gtccgtggtt gacggcgaag gccgcgtgca tgggctggaa ggcctgcgcg tggtggatgc
     5821 gtcaattatg ccgcagatta tcaccgggaa tttgaacgcc acgacgatta tgattggcga
     5881 gaaaatggcg gatatgattc gcgggaagga agcgttgccg aggagcacgg cgggatattt
     5941 tgtggcaaat gggatgccag taagagcgaa aaaaatgagt cgtgatttga actgactcag
     6001 gaaccgatca gattgagagt taccgttcca ggtatgggga cagaatcgtt atataaaaca
     6061 taggggtaac tctcatgctt catagtgaca acctcgccag caaattctaa gctgactcgt
     6121 tcaatctcgc tgatgaaggt actatgaatg cactgataaa ccgcaggtga aatgccactc
     6181 agcttaagaa ttacgccgct agtgttcctg gctatggtga cttgttcact cccgctcacg
     6241 gttagcatat acgaaaaggg ttcttgtaac gaggacaaaa ataagtcaga ccaaaacaac
     6301 gtggagcttt aaacacaatt cgtataattt attagcagtg atttattttt actcttcttt
     6361 aattaatcat catgcttaaa tattttttag tacaaggtta tcgcttcgct aacattttag
     6421 tttacattga tttccaattg acaataaaaa gttgttaata agtttatgca aaaaaaaata
     6481 aaaattgttg aaatactctc cagcaaatga atc
//
LOCUS       DAZGXS010000194         6510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGXS010000194 DAZGXS010000000
VERSION     DAZGXS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            89..652
                     /gene="fimA"
                     /locus_tag="ACHKG5_004446"
     CDS             89..652
                     /gene="fimA"
                     /locus_tag="ACHKG5_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0870416.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1013..1723
                     /locus_tag="ACHKG5_004447"
     CDS             1013..1723
                     /locus_tag="ACHKG5_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0870417.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1765..4416
                     /locus_tag="ACHKG5_004448"
     CDS             1765..4416
                     /locus_tag="ACHKG5_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0870418.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            4430..4960
                     /locus_tag="ACHKG5_004449"
     CDS             4430..4960
                     /locus_tag="ACHKG5_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870419.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4973..5476
                     /locus_tag="ACHKG5_004450"
     CDS             4973..5476
                     /locus_tag="ACHKG5_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870420.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5536..6450
                     /locus_tag="ACHKG5_004451"
     CDS             5536..6450
                     /locus_tag="ACHKG5_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870421.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1851 a         1406 c         1451 g         1802 t
ORIGIN      
        1 gaatgattct tgttttataa gcaataacag aatgacttaa ttgttaactc tctttattca
       61 gctacttaaa tataaatttt ggagaattat gaaactcaaa catgttggta tgattgtcgt
      121 ttctgtgttg gcgatgtcgt ctgctgcggt aagcgcagcc gagggtgatg aatcagtaac
      181 gaccactgtt aatggcggtg ttattcattt taaaggtgaa gtggtaaatg ccgcttgtgc
      241 gattgattcc gaatcaatga accaaacggt tgagctgggt caggttcgtt cttctcgcct
      301 ggctaaagcg ggtgacctca gctccgccgt tggcttcaat atcaagctga atgattgtga
      361 taccaatgtt tccagtaatg cagctgttgc attcctgggt actactgtca ccagtaatga
      421 cgatacgtta gcgctgcaga gttcagcggc aggctctgcc caaaatgtcg gtattcaaat
      481 tttggaccgt acgggtgagg tattaatact tgatggggcc acttttagtg ctaaaaccga
      541 cttgattgat ggcacgaata tactaccatt ccaggctcgt tatattgctc tcgggcagtc
      601 cgtagctggt actgcaaacg cagatgcgac cttcaaagtt caatatctat aattcaatat
      661 taaggtggct atacggcatc tgaaattaaa acagggtgtg tgtggcatgg ctacgcacac
      721 ccgagcaatt caccaatgtg aatctcatta ttaatgatga gtggtttgta tttggagcgc
      781 tatgaaaaat cacataatat ctaaacgttc aataaacaac agtagttaaa cttactgtta
      841 ttaaaaatat ttacttttta gcaaggtttt cctattaaaa gagaaccaac ctttattaca
      901 aacgcattaa gtctggaaac ccggtgactc gccaatgtat tagcaacatt taccgattgt
      961 ctgatgtaac ggttttcccg cttatcctct taacacatgg agaatgcatc agatgcaaac
     1021 tacgcgtacc ccctactcta tttcctttat ggcgacagtg ttacttttat tgctctttgc
     1081 ttgccatagc accgttgcca acgcagctgt cgcactgggt gcgacgcggg taatttatcc
     1141 ggcaaaccag aaacaggttt tattgcctgt cacgaataat gatcctgcca gtgtctatct
     1201 cattcagtca tggatagaaa atgctggtga ccaaaaagac acccagtttg tcattacccc
     1261 acctcttttt tccatgcaag gtaaaaaaga gaatacgctg cggataatca acgccactaa
     1321 tcatcagtta cccggcgacc gtgaaagcct gttctgggtt aatgttaaag ctattcctgc
     1381 tatggaaaaa gaccagaaga atgagaatac attacagctg gcaattatta gccgcataaa
     1441 aatgttttat cggcccacga acctggcaat ggcacctgaa gaagcgcctg caatgcttcg
     1501 gtttcgtcgc agcggtagca atctcactct tattaatcca acgccctact tcattaccgt
     1561 aacaaatatg aaagcaggaa atagcaatct cccaaacacc atggtgccgc caaaaggtga
     1621 agtgtcggtg gatatcccgc atgctgccac cggggatatc agttttcaga cgatcaatga
     1681 ttacggcgca ttaactccgc gtattaaggc aaccatgcag taagccagtg acctaattaa
     1741 taataaactc aatcggaatt aataatgaca gcctttcgtg ccgcatttaa ggcatatcgt
     1801 atgcatcagg tgttactact gcctcgcttt gcacggctta ccattgccct tagcttagca
     1861 actgcggtat ttcccgttga cgcagagtat tattttaatc cacggttttt aagcaatgat
     1921 cttgctgaat ctgtcgattt atccgccttt actaagggac gcgaggcacc acctggtaca
     1981 tatcgggtcg acatttattt gaacgacgaa tttatgacta gtcgggatat tacttttatt
     2041 acagatgata ataacgcaga tctcatccca tgcctgagta cagatcttct tgtcagtctt
     2101 ggcataaaaa aatctgcgtt attagacaat aaggaacatt cagcagaaaa acatgtgccg
     2161 gacaacagcg catgcacacc acttcaggat cgtttggtcg acgcatcaac tgagtttgat
     2221 gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag aatggctcgc
     2281 ggctatgttt ctcctgatct gtgggaagaa ggaataaatg ctgggcttct aaactatagt
     2341 tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc caactatgca
     2401 tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga taactcaacg
     2461 tggagttata acagtgggag tagcaattca tctgacagca ataaatggca gcatatcaat
     2521 acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg tgatagttat
     2581 accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa ttcaacagaa
     2641 gcgatgttgc ccgatagcca acatggtttc gctccggtga ttcatggtat tgcccgtggc
     2701 accgcacaag tgagtgtaaa acagaacgga tacgatgttt atcagactac tgttccaccc
     2761 ggccctttta ctattgatga tatcaactct gcggccaatg gtggtgatct gcaagtaacc
     2821 ataaaagagg cagacggcag tattcagaca ttatatgttc cttattcgtc tgttccggtt
     2881 ctccaacgtg ctggatatac gcgttatgcg cttgccatgg gggaatatcg tagtggaaat
     2941 aacctgcaaa gctcccccag gttcatacaa ggtagcttga tgcatggact ggaaggaaac
     3001 tggacacctt atggcggaat gcaaattgca gaagattatc aggccttcaa ccttggtatt
     3061 ggtaaagatt taggactttt tggtgccttt tctttcgata tcacgcaggc caatacgaca
     3121 cttgcagatg gcacccgtca cagcgggcaa tcggttaaat ccgtctacag caaatccttc
     3181 taccagacag gaaccaatat ccaggtcgca ggatatcgct attctacgca aggtttttat
     3241 aacttatccg acagtgccta cagtcgaatg agtggttaca ccgtcaagcc tcctaccgga
     3301 gacagcaatg agcagacaca atttattgat tattttaatc tgttctacag taagcgtggt
     3361 caggaacaaa taagcatctc tcagcagctt ggaaattacg gtgcgacatt tttcagtgcc
     3421 agtcgccaaa gttactggaa cacgtcacgc agcgaccagc aaatatcatt tggattaaat
     3481 gtgccgtttg gtgatattac gacttcgctg aattacagct attccaataa tatatggcaa
     3541 aacgatcggg atcatttact cgcttttacg cttaatgttc ccttcagtca ttggatgcgt
     3601 acagacagtc agtcggcatt tcgtaattca aacgccagtt acagtatgtc aaacgatttg
     3661 aaaggcggca tgaccaatct atcgggggtt tatggcactc tgctgccgga taataacctg
     3721 aattatagcg ttcaggtcgg taacacccac ggaggtaata catcgtctgg caccagtggt
     3781 tacagtactc ttaattatcg tggagcttac ggcaatacta atgtcggtta cagtcggagt
     3841 ggtgacagca gccagattta ttacggaatg agtggtggga ttattgctca tgctgatggc
     3901 atcacctttg gacagccgct gggcgacaca atggttctgg ttaaggctcc tggcgctgat
     3961 aatgtcaaaa tagagaacca gaccggaatt cataccgact ggcgtggcta tgccatatta
     4021 ccatttgcga cagaatatag agaaaatcgt gtcgctctta acgcgaattc ccttgcagat
     4081 aatgttgaac tggatgaaac cgtggtcact gtcatcccaa ctcacggtgc tattgccaga
     4141 gcaacattta atgcacaaat cggcgggaaa gtattaatga cgttgaagta cggtaataaa
     4201 agcgttccat tcggtgcaat tgtcactcac ggagagaata aaaatggcag cattgtcgcg
     4261 gaaaacggtc aggtttatct gactggactt ccacagtcag ggaaattaca ggtttcatgg
     4321 ggcaatgata aaaactcaaa ctgtattgtc gattacaagc ttcctgaagt ctctcctgga
     4381 accttgctga accagcagac agcaatctgt cgctaaggaa caatagatta tgaaatacaa
     4441 taacattatt ttcctcggtt tatgtctggg gttaaccacc tattctgctt tatccgcaga
     4501 tagcgttatt aaaattagcg ggcgcgtcct cgattatggc tgcacagtct catcggattc
     4561 gcttaatttt accgtagatc tccaaaaaaa cagtgccaga caatttccaa cgaccggtag
     4621 cacaagtcca gccgtccctt ttcagattac gttaagtgaa tgcagcaaag ggacaacggg
     4681 ggttcgggtt gcatttaacg gtattgagga cgcagaaaat aatactctgt tgaaactgga
     4741 tgagggaagc aatacggcct ccggtttagg tatagaaata ctggacggaa atatgcgtcc
     4801 ggtgaaactg aatgaccttc atgccgggat gcagtggatc ccactggtac cagaacagaa
     4861 caatattttg ccttactccg ctcgtctgaa gtcaactcag aagtccgtca atccgggact
     4921 ggtgagggct tcggcaacct ttacccttga atttcaataa tcagagtcag atatgaaaag
     4981 acttcacaag aggttcctct tagctacgtt ttgcgcgtta ttaacagcaa ctctccaggc
     5041 cgccgatgtc actatcactg ttaatggtcg ggtagtcgct aaaccctgca ctattcaaac
     5101 caaagaagct aacgttaatc tcggggatct ttatacgcgc aatctgcaac aacctggttc
     5161 tgcatctggc tggcacaata ttactttgtc attaaccgat tgtccggctg aaacaagtgc
     5221 agtgacggca atcgtgacag gttcaactga caatacgggt tattacaaaa atgaaggtac
     5281 tgccgaaaat attcagatag agcttaggga tgaccaggat gcgacgttaa aaaatggcga
     5341 tagcaaaacg gttattgttg atgagatcac tcgtaatgca cagtttccac ttaaggcaag
     5401 agctatcacg gtgaatggaa acgcaagcca gggaacgatc gaggcgctaa tcaatgtgat
     5461 ctacacctgg caataacgtc gattcgattt tcttagcctt ctttttaaat caatttttta
     5521 accttaggat atatcatggg taaaacaatc agtataaagg tcctgttcgg tatttatctt
     5581 ctattaatgg caggcaaagt ttttgccttc agttgtaatg tcgatggcgg gtcaagtatt
     5641 ggagctggta caacctctgt ttatgttaat ctcgaccctg taatacagcc gggccagaat
     5701 ctggttgtag acttgtctca gcatataagt tgctggaatg attacggcgg ctggtacgac
     5761 actgatcata taaacctggt acaaggctca gctttcgcag gctctctaca gtcatataaa
     5821 ggatcgctgt actggaataa tgtgacctac cccttcccgt tgacgacaaa taccaacgta
     5881 ttggatattg gtgataagac accgatgcca ttaccgttaa aactctatat taccccagtc
     5941 ggggcggccg gtggagttgt cataaaagcc ggggaagtta ttgcccgtat tcatatgtat
     6001 aaaattgcca cgttaggaag cggaaatccg cgtaatttta cgtggaatat tatttcaaat
     6061 aacagtgttg ttatgccaac aggcggctgc actgttgata gtcgtaatgt caccgtgaat
     6121 ctcccggact ttcctggaag cgcagaaatc ccccttggcg tatattgctc aagcgaacaa
     6181 aagctctcat tttatctttc gggtactacc actgacagtg ctagacaagt gtttgctaac
     6241 acagcaccag atgcaacaaa agcatcggga gtgggtgtct ctctgatgcg taatggaaaa
     6301 atactggcta cgggagaaaa tgtctccctg gggacagtaa ataaaagcaa agtacctctg
     6361 ggtttaagcg caacctatgg acaaacaggg aacaaagttt cagccggaac cgtccagtca
     6421 gtcattggtg tcacttttat ctatgaataa ttgtgatttt gaccttagtc tatgcgctcc
     6481 attcacgaaa attgcgcgga taattatttt
//
LOCUS       DAZGXS010000195         6464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGXS010000195 DAZGXS010000000
VERSION     DAZGXS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(359..1495)
                     /locus_tag="ACHKG5_004452"
     CDS             complement(359..1495)
                     /locus_tag="ACHKG5_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0870422.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1498..1860)
                     /locus_tag="ACHKG5_004453"
     CDS             complement(1498..1860)
                     /locus_tag="ACHKG5_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0870423.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2397..4310
                     /gene="mtlA"
                     /locus_tag="ACHKG5_004454"
     CDS             2397..4310
                     /gene="mtlA"
                     /locus_tag="ACHKG5_004454"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0870424.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4405..5553
                     /gene="mtlD"
                     /locus_tag="ACHKG5_004455"
     CDS             4405..5553
                     /gene="mtlD"
                     /locus_tag="ACHKG5_004455"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0870425.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDSLNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5553..6140
                     /gene="mtlR"
                     /locus_tag="ACHKG5_004456"
     CDS             5553..6140
                     /gene="mtlR"
                     /locus_tag="ACHKG5_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0870426.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6152..6361)
                     /gene="yibT"
                     /locus_tag="ACHKG5_004457"
     CDS             complement(6152..6361)
                     /gene="yibT"
                     /locus_tag="ACHKG5_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0870427.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1625 a         1635 c         1648 g         1556 t
ORIGIN      
        1 agttgtatac atatattcca tgaccctaca ttgtgatcta tagattaaat attttttaaa
       61 tgtgactgtc atccttcaga taataaagcc atcatcgcaa tagatggaaa agcactcctg
      121 ctcttttttg attagagccg tcgcaggagg aacaactcat atcatcattg cgatctcgac
      181 gatgtatagt ctcatcctgc ggcagaacaa gacggataaa aaatccaatg agagcacact
      241 catccagaac ttattaacat aatgaatatt aaagaaaaat catcgcagct aatgaagtaa
      301 tatgcaaaaa taattagtcc ttacaaggag caaagttcca taatctcccc cctccccctc
      361 aatgatccaa ataaagataa tgcatccagc tggtcattct tagcagcact ttccgcatca
      421 ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg ggcgtagatg ccgtcgggta
      481 aggcatcgat atcatcgtta gggtccagtt caatggttcc cagcacaccg tccgtgccgg
      541 gcacgaccgt taatgattgc aataccccct gcgcctgata agaaccgcct ggcacgacag
      601 gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg tagcgcgtta aacaccactt
      661 ccgcatcatc gccgggtttc agacgtaaca gcgagttttg ccgaaattgg gcgacaattt
      721 gccgtttttg ctcggggatg aagaccatca ccggacgcag cggcaaggca gctgcgtatg
      781 tacctgggcg gatcagtacc tgagtaacgt agccattgct cggcgcgcga atgacagtct
      841 gctcaaggtt atattttgct tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct
      901 cgccgttaac catactgtcg agctggctct ggatctgcgc ctgctccgcc accgagcctt
      961 tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga
     1021 acggattcac cgccgcctgg ctgcctttca agtaacgttg ataattttta aacagacggt
     1081 cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc gcacgcagcg
     1141 tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt
     1201 aacgaaccgg gtcgagctta aaaagcacct cgcccttttg aataagctga ttattcttgt
     1261 cagtgacttc agtaacaatt cccgtcacct gtggcgtgat agggatcgct atcactgcct
     1321 tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa taaaatcaaa ccactcacca
     1381 gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg atttaccgga atgcggaaga
     1441 ttttaaagac cgcccacgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     1501 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     1561 ggaaattcgg tcagaaagtg aggcgatttc cgggtcagtc gctttctcct gcgacgcaac
     1621 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     1681 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     1741 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     1801 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     1861 atctgcctcg gattcacgtt tatcagtgtt gtttttgggc tggcagccag aagggagtca
     1921 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     1981 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     2041 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     2101 acaaaccatc aaaacaaaaa tgtgacacta ctcacattta aatgccattt ttagcgaaaa
     2161 tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg aacgtcacgt caattacctc
     2221 tctaccccct atatttatgt gattgatatc acacaaaagg ccgtcgactg gacagttaac
     2281 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     2341 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     2401 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     2461 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     2521 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctgccgc
     2581 tgctgatcgg ttataccggt ggtaagctgg taggcggcga acgtggcggc gtagtcggtg
     2641 ccatcaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
     2701 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
     2761 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
     2821 tcgctattct ggcattcctc ggcattggcc cgattgttga aaccctgtcc aaaatgctgg
     2881 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
     2941 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
     3001 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
     3061 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
     3121 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
     3181 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
     3241 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
     3301 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
     3361 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
     3421 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggtg
     3481 catctccgct gtctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
     3541 ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa
     3601 ttcaggatgc aggtctgtca cagatttcgg tcactaacag cgcgatcaac aacctgccgc
     3661 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
     3721 ctcaggcaca gcatatttcg ctaaccaact tcctcgacag cggcctgtac accagcctga
     3781 ctgaacgtct ggttgctgcc cagcgacata ctgaaaacga agtgaaagtg aaagacagtc
     3841 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
     3901 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
     3961 tgaaaggcgg ttacgttgag ccggagtacg ttcaggcgat gctggatcgt gaaaaactga
     4021 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
     4081 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
     4141 aagaagatga cattgctcgt ctggtgattg gtattgctgc ccgtaacaac gagcacattc
     4201 aggttatcac cagcctgacc aacgcactgg atgatgagtc cgtcatcgag cgtctggcac
     4261 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
     4321 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
     4381 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
     4441 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
     4501 aatcaggtgg tacttgattc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
     4561 gaaaccgagc aggtagatac cgtttccggc gtcgatgctg tcagcagcat tggtgatgat
     4621 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
     4681 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gtaaagaaca aggtaatgaa
     4741 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gtaccacgca gctgaaaggt
     4801 catgtgatga acgccctacc agaagacgcc aaagcgtggg tagaagaaca cgttggcttt
     4861 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
     4921 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
     4981 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
     5041 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
     5101 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
     5161 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
     5221 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
     5281 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccattgctc
     5341 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
     5401 cacttccgca gtgaagacga tccgcaggct caggaactgg cagcactgat cgctgacaaa
     5461 ggtccgcagg cggcgctggc acagatttcc gatcttgatg ccaacagcga ggttgtatcc
     5521 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
     5581 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gcctttgaaa
     5641 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
     5701 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
     5761 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
     5821 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
     5881 cggagctgct gatggcattg cgtgaagaac taaatcacga cggcaacgag tacgccttta
     5941 ccgacgacga gatccttgga ccctttgggg aactgcactg cgtggcggcg ttaccaccgc
     6001 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
     6061 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaga atcagcttaa
     6121 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
     6181 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
     6241 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
     6301 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
     6361 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
     6421 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caat
//
LOCUS       DAZGXS010000196         6370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGXS010000196 DAZGXS010000000
VERSION     DAZGXS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..360
                     /gene="ydjO"
                     /locus_tag="ACHKG5_004458"
                     /pseudo
     CDS             1..360
                     /gene="ydjO"
                     /locus_tag="ACHKG5_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(364..1755)
                     /gene="tcyP"
                     /locus_tag="ACHKG5_004459"
     CDS             complement(364..1755)
                     /gene="tcyP"
                     /locus_tag="ACHKG5_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0870428.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILGSEDDAELAHH"
     gene            complement(1888..2478)
                     /gene="ydjM"
                     /locus_tag="ACHKG5_004460"
     CDS             complement(1888..2478)
                     /gene="ydjM"
                     /locus_tag="ACHKG5_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0870429.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2641..3309)
                     /gene="hxpB"
                     /locus_tag="ACHKG5_004461"
     CDS             complement(2641..3309)
                     /gene="hxpB"
                     /locus_tag="ACHKG5_004461"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0870430.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3456..3992
                     /gene="yniB"
                     /locus_tag="ACHKG5_004462"
     CDS             3456..3992
                     /gene="yniB"
                     /locus_tag="ACHKG5_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0870431.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4033..4893)
                     /gene="yniA"
                     /locus_tag="ACHKG5_004463"
     CDS             complement(4033..4893)
                     /gene="yniA"
                     /locus_tag="ACHKG5_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0870432.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4999..5289)
                     /gene="ghoS"
                     /locus_tag="ACHKG5_004464"
     CDS             complement(4999..5289)
                     /gene="ghoS"
                     /locus_tag="ACHKG5_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0870433.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5390..6319)
                     /gene="pfkB"
                     /locus_tag="ACHKG5_004465"
     CDS             complement(5390..6319)
                     /gene="pfkB"
                     /locus_tag="ACHKG5_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0870434.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1641 a         1592 c         1565 g         1572 t
ORIGIN      
        1 acatcctgtc gggagcattt ttgctgcgat tcaaaaaaat gagagtctcc taaagtttcc
       61 ttcgataata tcaatgctta atatcaatct tctttttaat ccactaaatc ttccgggaat
      121 gggaagtggt atacttgaag atattatgag tattccagat agttcactta gaaaacgtct
      181 cgggtatgag gttttaagct tctcgctaca ggcacattca ttgagccagg aatgtattga
      241 taagttggac attttttcgc tgacgatctg tttaaatatg agtcagtatg catcgccgcg
      301 atggaacatt taaaaagtaa ggcaacggcc cctttacaaa acggaccgtt gccagcataa
      361 gaattaatgg tgtgccagtt cggcgtcgtc ttcgctaccc agaatggctt tatcggtttg
      421 cttcagccac tggctggtca gcgtgccagc tgtcatcgag ccactaacgt ttagcgccgt
      481 acggcccatg tcgataagcg gttcaacgga gattaacagc gccaccaggg ttactggcag
      541 gcccatcgca ggcagcacaa tcagtgcggc gaaagttgca ccaccaccga caccggcaac
      601 gcctgcggag ctaacggtaa caataccgac cagcgtcgca atccacatcg ggtccagcgg
      661 gttaatgcca accgtaggcg caaccatcac cgccagcatt gccggataca aaccggcgca
      721 gccgttctga ccaatggtcg caccgaaaga ggcggcgaaa ctggcgatgg attcaggaac
      781 accgagtcga cgcgtttgtg cttccacatt cagtgggata gacgcagcac tggaacggct
      841 ggtaaaggca aacgtcagca caggccatac cttacggaag tacttcagcg gactcacgcc
      901 attaatgccc agcagaatgc catgcactgc aaacataatc agcagaccga ggtaggacgc
      961 gacaacgaaa cttcccagtt tgatgatgtc ttgcaggtta gaacctgcaa ccactttggt
     1021 cattagtgcc agaacgccgt aaggggtcaa ctgcatgacc aggcgaacta gtttcatcac
     1081 ccagctttgc agggtatcga tagcggttaa gacgcgttca cctttcggcg catcatcctt
     1141 cagcagtttc agcgcagcta caccgaggaa tgcggcaaaa attaccacgc tgataattga
     1201 cgtcggattg gctccggtca gatcggcaaa cgggtttttc gggataaagg acaagaccag
     1261 ctgcggaacg ctcaggtcag agactttacc aacatagtta ctttcgatgg cgttcagacg
     1321 tgcagtttct gcaccaccct gaaccagacc ttcagccgtc aaaccaaaca ggttggttac
     1381 cagcacaccg accagcgccg caatcagcgt ggtaaacaaa agcgtaccaa tggtcagaaa
     1441 actgattttg cctaactgag atgcgttatg cagacgggca accgcgctca gaatagaggc
     1501 gaagactaac ggcataacga tcatttgcag cagttgaaca tagccgttac caacaatgtt
     1561 aaaccactgt acagaatctt taagtacctg gctgtcagaa ccataaatgg tatgcagggc
     1621 aaggccaaaa accacaccca tcaccagacc caccagcact tttttcgcca gactccactg
     1681 tttatggcgg gtctgagcca gcgcaaacag cagtacaacg aacaccacga tgttcgcaat
     1741 taatggaaag ttcatccccg ttctcctgat cttattattt ctatcactgc gtagcagtca
     1801 tagtgttggc gcaagattaa cagaactgtg agctggctct tatattcaaa tggaattgct
     1861 tattacagaa aggcgatttt tgccttttta gtattcaacc tgatgcttaa taagccgatg
     1921 aaactggatc tgcaaggtat tgatcatttg cgatgaccaa cgaacagcgc tgttttcggg
     1981 taatgaatga ggcatccata ccgaccagac aaataatgcc atgcagataa aacgttccag
     2041 ttggttaccc ttttggggaa ccaggatagg caagcggaaa cgccagcggc atggccagag
     2101 caggggaaca ccggcgggtg tcagcatatc ggcaagtatg tggcttaaat aacccagcac
     2161 cattccttgt agcgcatcag ccggaatgaa ccagctttca ggaaccttaa ggtaaaaggt
     2221 tgccagcagc gcaaataccg ccagcagact gtgggtaaaa ccacgatgcc caaaagcgcg
     2281 ggcgatcggt tttgatatcc actttaatcg ctgcccaaga aacgactttg ggtgatcgat
     2341 gtccggtaac aaacacgtca ggattgcgga agggacaata tgccaccagt caccctgtgc
     2401 cagcacgggc gtcagctcgg catttttggc aaataccgca caagcaatag aaaagagaag
     2461 gtgaccttcc gccgtcatga taaaactcac taaactgtcg atacgtacag tataggattt
     2521 ttatacagtg cacggaagtg gtgacggtgt aactctttct cttaaattga cgattaatcc
     2581 gtcagttgaa tgcaaattca gataaagaac caggggcagc gtgatcactg cccctggtca
     2641 ttaaccgaga aggtcttttg cggtgagttc tgtcagcgat gagagtttga cgtttgctaa
     2701 tacaaaacgt ggatcatttt gcgcttctgg cgcaggaacg acgatggaac gcatgcgggc
     2761 tgctttagag gcgatcatgc catttaccga atcctccagc gctacgcagg tcagtgggtc
     2821 aacgcccagt tttgctgcgc agtcgagata tacttgcgga tgcggcttgc tgtaaggcag
     2881 tttttcggcc gaggcgaggg catcgaaact gtcgcgtaag tcaaacatgg tcaacacttt
     2941 ttccagcata tgtagtggtg acgcggaggc cagtcccacc aataaacctt gttctttgca
     3001 taacgcaacg gcttcgcgca cgcctggtaa taatggacgt gtctcttcaa ccagtgaaat
     3061 ggcacgggca ataacccgtt ctactacttc ctgacggctt ggcccattcc acggttgccg
     3121 ggcgtaccaa agatcgacca ccatatcgat gcgtaaacct aaggtgtccg gcagctcgtt
     3181 acgacgggag atatccaccc ccaggcttgc catcacatcc agttcggctc gatcccataa
     3241 aggttctgag tcgataagta atccatccat atcaaaaatt gcagcaagaa tctgacgcgg
     3301 ggttgacatt acaacctctc ctttagagca ttatgtgtaa ggtattaagc cgtgcttatg
     3361 attaagcgcc tatcatatcg cctttaaaga atgggcgaaa acggatatct acaggtaaac
     3421 taagtgaaac gacgcgtctt aatcaaggag aactcatgac gtatcaacaa gctggacgca
     3481 ttgctgtttt gaaaaggatt atgggttggg tgatttttat acctgcattg atctctacat
     3541 tgatttcttt gctgaaattt atgaataccc ggcaggaaaa ccaggaaggc attaatgcgg
     3601 tcatgctcga ctttactcat gtcatgatcg atatgatgca ggcgaatacg ccttttttga
     3661 atctgttctg gtataactcc ccgacgccca attttaatgg tggcgtgaac gttatgttct
     3721 gggtgatttt catcctgatt tttgttggac tggcgttgca ggattccggt acccggatga
     3781 gtcgccaggc gcgcttcttg cgggaaggtg ttgaagatca actcattctg gaaaaagcca
     3841 aaggggaaga ggggctaacg cgtgaacaaa ttgagtcacg tattgttgtt ccgcaccata
     3901 ctattttcct gcagtttttc tcactgtata tcctgccggt tatttgtatt gctgcaggtt
     3961 atgtgttctt ttctctgctt gggtttattt aatcaaaagg ccgcacggag cggccattat
     4021 cttcaaccca tatcacgcag ctaataatct atccagtgac tgctgagcaa taaccaaatg
     4081 ctgaccgcca aataatcttg cacgatttaa cagcgtgtag agttggtaaa ccggttgacg
     4141 ttcaaggaaa tcggcaggta gcggggatac tgactgatag ccgtcataga tttgtggcgg
     4201 ctgttcagta tgcagcggta acatcgccag gtcgcactct cggtcacccc agtagcaggc
     4261 cgggtcgaaa atgtacgggc catccggacc cagcgcacag ttgccggacc ataaatcgcc
     4321 gtgcaacaga gaaggctgcg gttgatgtga ggcgagacgc tgctgaatat gctcgacgat
     4381 ggcatcgata ttgccgaaag cgatcccttt ttctgctgcc agttccaact gccagccaat
     4441 ccgttgttca gcaaaaaacg ttgaccagcg acgttgccag gtgttgggct gtggagttgt
     4501 ggagagcgag ttatcaaaat cgagtccaaa ttgtggttgg tcgctccatt gatgtaaacg
     4561 cgcaatttgc tgaccaagaa taaatgcgct atgcgcatcc agcggacgag gtgggagata
     4621 atccatcacc agaaaactgt agtcacggtc agcgccaact gcccaaacct taggcacggt
     4681 gacggtttta ctacgcgaca gtaactccag ttggtcggct tcggcggtaa aaccgggaag
     4741 cagttccctt tcatcacatt tgacgaaaaa gtcatgtcct gcatagcgca aatgccatgc
     4801 ggcatggact tctccgccag gcagttcatt acgcagttcg atttcgcctt cacctaactg
     4861 ctcgctcaaa agacgactga ttgcctgcca catgatgtct ctcccatgtt gtctgccaga
     4921 tcataaggtt agcgcaagaa tgcggtgtaa aaacacgagc taacgcacac cacagactga
     4981 ttattgccca aaaaccattt attttctgtt gctattccat tcctcccagg tcgtgatggt
     5041 gatttcagga tctttgccgg ttgcactttg ggtaagcccc gaaaccagtt ctctaatttc
     5101 ttcttcactt tgggtactga taagtccaaa agtgttagtc cccagctcat ggatatttcc
     5161 ctcatcatcc gtcatggtga gcaaaaaacc gtcgcgagtc aggtaattat tcaactcgtt
     5221 aatttcagtc aatgtatcct catgcaacat gacggttatg acataacgga caagatcgcc
     5281 actggccata gttcacccct ttgttatcac atgacttttg agcatagtcg gagaaacgcg
     5341 ttgccgacag gttggtgatg attcccccaa tgctggggga atgtttttgt tagcgggaaa
     5401 ggtaagcgta aattttttgc gtatcgtcat gggagcacag acgtgttccc tgattgagtg
     5461 tggctgcact ccccgcagct acgccaaaac gaaccatctc ttcaagagag gcattttctg
     5521 ccagtttcag tgtcatcgcg ccgaccatgc tatcaccagc gccaacggta ctctggcttt
     5581 tcaccggtgg tggcaccacc tgaatgcagt tttcactatc aacacccagc gctccttgtg
     5641 gacccaggga aacgacaacc cgtttggcct tgccgctatt aacgatttcc tgcgcggctt
     5701 tgcggacatc gtctggctgg gtgagttcgc gattcaccag cgcactgagt tctttttggt
     5761 taggcttaac caactcgatg ttaccaatcg ccagtgctgc acttaacgct tcgccagaac
     5821 tgtcgatgat gcagcggatc ccttggtttt gcgcagcgga aatcagttgg gttaattttt
     5881 ccagcttcac acctggcggc aggcttccgc ttatgaccag gatggccccg gattcaattt
     5941 ccagaacttg ctcttcaagc tggcgaaact catcttcatt taatgccgcg cctggcataa
     6001 caaaacgata ctgctcaccg cttgcttcca catgtacgtg taaattctgc cgggtccagt
     6061 ctttggcttc tacagtagcg acggggacat tttcatccgc caacagtgaa accaggtgtt
     6121 cgccggtcgc gccacccgcc gggaagatcg ctgtggcact gcctccaaga tgggcaatgg
     6181 cgcgggcgac gttgatgccg ccgcccccgg gttcgaacac cggtgcggta cagcgcagtt
     6241 ttccttcggg ataaatttgc ggggtaattg ttgcgctatc gagagagggc gcaagtgtca
     6301 acgtatagat acgtaccatc atttcctcct ataggctgat ttcagtctgg caccgaatca
     6361 gcggaaagtg
//
LOCUS       DAZGXS010000197         6331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGXS010000197 DAZGXS010000000
VERSION     DAZGXS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(26..889)
                     /gene="potB"
                     /locus_tag="ACHKG5_004466"
     CDS             complement(26..889)
                     /gene="potB"
                     /locus_tag="ACHKG5_004466"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0870435.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(873..2009)
                     /gene="potA"
                     /locus_tag="ACHKG5_004467"
     CDS             complement(873..2009)
                     /gene="potA"
                     /locus_tag="ACHKG5_004467"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0870436.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2259..3485
                     /gene="pepT"
                     /locus_tag="ACHKG5_004468"
     CDS             2259..3485
                     /gene="pepT"
                     /locus_tag="ACHKG5_004468"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0870437.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3534..4655)
                     /gene="roxA"
                     /locus_tag="ACHKG5_004469"
                     /gene_synonym="ycfD"
     CDS             complement(3534..4655)
                     /gene="roxA"
                     /locus_tag="ACHKG5_004469"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0870438.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            4904..6133
                     /locus_tag="ACHKG5_004470"
     CDS             4904..6133
                     /locus_tag="ACHKG5_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0870439.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
BASE COUNT         1668 a         1521 c         1637 g         1505 t
ORIGIN      
        1 ctattaacct tttgaatata aagcactaat tatctatttc tgttgccttc ttattcagca
       61 gacgagaagc gcgccagtaa accagcaaca tcaggcccat tacgatagtc agcgtaatgc
      121 tggtggctgc accaaacggc cagtcacgaa tattgaggaa ctgtaccttg atgacgttac
      181 cgatcagcag gtttttcgca ccgcccatca ggtcggacac atagaacagg cccatcgctg
      241 gcagcatcac cagcagacat ccggcaataa tccccggcat cgtcagtgga ataatgatgc
      301 ggataaaagt ctgtaacttg ctggcaccga gatcgcgcgc cgcctcaagc aacggcttat
      361 ccagtttttc gatactggag tacagcggca tcaccataaa cggtagcaga atgtaaacca
      421 gaccgataat caccgcactg ggcgtgaaca tgatacgaat tggtgtgtcg ataacgccca
      481 gccagagcaa aaactcgttg agatagcctt tggtgctgag gaaaattttc agcccgtaga
      541 tgcgaatcag tgagttggtc cagaagggaa caatcagcag aaacagcagc agcggacgca
      601 ccttgtgtgg cagcttcgcc agaaaccagg caaacggata gcccagcacc aggcaggcga
      661 gggtggcgat cagcgccata ttcagcgagt gcaatagcac ttcaaaatag agcggatcga
      721 gcagacgtgt gtagttatcc agcgtaaaga ccattttgac aaaactggcg tcgtcgcggg
      781 ccaaaaagct ggtgccaatg atcatcaggt tgggcagaaa gacaaacaac acaagccaac
      841 cgacaatagt gacaatcact acattctgga acttacttgt gttcttcatc agccagtacg
      901 acctcccagc tttctaccca attaatggcc attttttggt cgagagagtg gtcaaagtca
      961 ggatcgtctt cattgaagaa ttcgctgacc atcaccatct tgccattttc cagttcaaca
     1021 accgactcca gcgtcatgcc tttgtagtta cgctcgcgaa cgtaaccaat cagcccttca
     1081 gcgtggttgt cgtcgttaat ctcttcaaca cgtaagtctt ccgggcgcag cagaacatgc
     1141 agtttttgcc ccggttcaac ggcgaagtta acgtagatat tacattcgcg gccttcaacg
     1201 ttggcgcgta cgcgctgctc gtcgagacgt tcgatgacag tggcgttaaa catattgatt
     1261 tcgccaatga acccggcaac aaacaggttt ttcggctctt cgtagatttc acgcggcgtg
     1321 ccgtcttgct caatgcgacc atcgcgcatc accacaatcc tgtctgacat ggtgagtgct
     1381 tcttcctgat cgtgagtcac gaagacgaat gtaatgccaa gcttacgctg taacgctttc
     1441 agctcgttct gcatttgctt acgcagtttg taatccagcg ctgagagcga ctcatccagc
     1501 aacaacagac gaggcttgtt aaccaccgcg cgagcaatgg cgacgcgttg ctgttgacca
     1561 ccagagagct gatgcggttt acgttgagcg aaggtttcca gctgcaccat ccgcagggct
     1621 tccatcacgc ggggcgtaat ttcagcagcg ggggtttttt gcatgcgcaa cccaaaggcc
     1681 acattttcga acacggtcat gtgggggaaa agtgcgtagc tttggaaaac agtgttcaca
     1741 tagcggtttt ccgccggaac gtgggtgatg tcctcgttat ccaacatgat gcgtccggaa
     1801 tcaacagttt ccagacctgc aatcaggcga agaacggttg ttttaccgca gccagaaggg
     1861 ccaagcagcg tgaggaactc gccattgttg atagtcagat ccagctgggg aatgacctct
     1921 ttaccatcaa agcatttgcg aattcccgcc aattgcacca gcggtgaaag cgaactcggt
     1981 tgtttattca attttttact ctgtcccatg taaacgcaac ggatggctta ccgatgcggg
     2041 gtttgtggtt aaccaccttg gtgactctta atgagggcgg taattctacg gcaaaccgct
     2101 tgaatcgcca atctttgttg tgaattactg gcttagcttt atattcatta aggtaatgct
     2161 gataaatatt cccgcttgca ggggtaaaag tgacctgacg caatatttgt cttttcttgc
     2221 ttcttaataa tgttgtcaca aaaagtgagg gtgactacat ggataaacta cttgagcgat
     2281 ttttgaacta cgtgtctctg gatacccaat caaaagcagg ggtgagacag gttcccagca
     2341 cggaaggcca atggaagtta ttgcatctgc tgaaagagca gctcgaagag atggggctta
     2401 tcaatgtgac cttaagtgag aagggcactt tgatggcgac gttaccggct aacgtccctg
     2461 gcgatatccc ggcgattggc tttatttctc atgtggatac ctcaccggat tgcagcggca
     2521 aaaatgtgaa tccgcaaatt gttgaaaact atcgcggtgg cgatattgcg ctgggtatcg
     2581 gcgatgaagt tttatcaccg gttatgttcc cggtgctgca tcagctactg ggtcagacgc
     2641 tgattaccac cgatggtaaa accttgttag gtgccgatga caaagcaggt attgcagaaa
     2701 tcatgaccgc gctggcggta ttgcaacaga aaaacattcc gcatggtgat attcgcgtcg
     2761 cctttacccc ggatgaagaa gtgggcaaag gggcgaaaca ttttgatgtt gatgccttcg
     2821 atgcccgctg ggcttacact gttgatggtg gtggcgtagg cgaactggag tttgaaaact
     2881 tcaacgccgc atcggtcaat atcaaaattg tcggtaacaa tgttcatccg ggcacggcga
     2941 aaggagtgat ggtaaatgcg ctgtcgctgg cggcacgtat tcatgcggaa gttccggcgg
     3001 atgaaagccc ggaaatgaca gaaggctatg aaggtttcta tcacctggcg agcatgaaag
     3061 gcaccgttga acgggccgat atgcactaca tcatccgtga tttcgaccgt aaacagtttg
     3121 aagcgcgtaa acgtaaaatg atggagatcg ccaaaaaagt gggcaaaggg ttacatcctg
     3181 attgctacat tgaattggtg attgaagaca gttactacaa tatgcgcgag aaagtggttg
     3241 agcatccgca tattctcgat atcgcccagc aggcgatgcg tgactgcgat attgaaccgg
     3301 aactgaaacc gatccgcggc ggtaccgacg gcgcgcagtt gtcgtttatg ggattaccgt
     3361 gcccgaacct gttcactggc ggttacaact atcatggtaa gcatgagttt gtgactctgg
     3421 aaggtatgga aaaagcggtg caggtgatcg tccgtattgc cgagttaacg gcgcaacgga
     3481 agtaagcgaa aagggatgcg gcatgtgatg ccgcatccgg cttaaatcca aacttaccct
     3541 tcgaagaacc aatacccgct attgaccagc gccgcgagca tcgcgaggaa tgacggatct
     3601 tccagcgcat cgccaaaatt ctccgcagtc agcgcaatgt tgctggcgag tgcatccagt
     3661 gccggacggt gcggggaatc gatcttctca ccattggtat acacgtcgtc gccaatgcgc
     3721 aatacgcgca gaccacccag gcgcaccagc acatcacctt gtttcagcgc atcgtagatt
     3781 tcatctggct gataaggcgg ctccggcggc gcgatatcca gttcatgacg tgactgggat
     3841 ataaactcgc caaaccattg cttaaagtgt tccggctggt tgatcaattc gagcatcatc
     3901 tcacgcagtt tatccatctc ttgcggcaga acgtccgcag gatgagcgcg aggtggaaca
     3961 tcaggatcgc tgtagtagtt gccgcccagt tcgcgttgca gcacataatc ggcaaatccg
     4021 ctaattaatt cccgcgtatt tggcgcgcga aaacccacgg aatagttcat cgcattttcc
     4081 agcgcgtagc cttcatgcgg gaatcctggc ggaatataaa gaatatcgcc aggctccagc
     4141 tcttcatcga tgatggcttc gaacggatcg acctgtaaca gatccggatg tgggcagtgc
     4201 tgtttcattt gcagcttctc gcccactcgc cagcgacgac gtccggtacc ctgaatgata
     4261 aacacgtcgt actgatcgag atgcgggccg acgccgccgc caggtacaga aaaagagatc
     4321 atcagatcat caatacgcca gtccggtagt tcacggaacg gtcgcatcag cgcggcggtc
     4381 ggctcatgcc agtggttcac tgcttgcact aacaatgacc agttagtttc accgagatga
     4441 tcgtagcttt cgaacggacc gtggctgacc tgccattttc catcctggtg actgaccagt
     4501 cgactgtcga cttcgctttc catcgccaga cccgccaact cgtctggaga gatcgggtca
     4561 ataaaattat taaagccgcg ttttaacacc accgggcgtt tctgccagtg acgttcaaga
     4621 aaatcgggcc agttaagagt gagttggtat tccatgttaa gcatccacag gctggtatct
     4681 gcaaccgatt ataacggatg cttaacgtaa tgcgtgaagt atggacatat tttatcaatt
     4741 agtgtttttt gccgtttcat aatgtttcag attgttcatt atatttttga ttataaacag
     4801 atttgtgatt ttccttgttc cagcttgttt cacgttgtag ccctttgtgc aacgatcggt
     4861 gtagttaatg gtgtagttaa ttgtgatgac cggaatcaaa attatgagca gagcacttaa
     4921 caaactgagc gatacacagc tgaggaaaat caacggcaca cccgcccaaa aaacagcctt
     4981 tcttaatgac ggtggaaacc tgagcgtcag gcattcaacc agtggccttt taacctggta
     5041 tttcacttac agggccggaa cgggaagggg ggcaccaccg gaacgcatta agctgggaaa
     5101 ttatcctgat ctgagcctga aatcagccag ggaaaaagcc gcccagtgtc gcgcatggct
     5161 ggcagagggg aaaaatccac gtcatgagct taattacacc gtacaggaag cgttaaagcc
     5221 ggtaacggtt ggcgatgcgc tcacctactg gcttgagtcg tacgcaaagg aaaaccgcgt
     5281 ggattatgcc gccctgaaaa agcgccttaa taatcacgta atacagcaca ttggtgctat
     5341 gccgctggat aaatgcgagc tacggcactg gctggcctgt tttgaccagg tggcaaagcg
     5401 aacgcctgtt actgccggat tcttgctaca gacgtgcaaa caggcgctta agttctgccg
     5461 gaggcggcgc tatgcaatca gcaacgttct tgatgatatg agtgtggcgg acgttgggaa
     5521 aaaaccggat ataagcgagc gtgtcttaag caccaaagaa ctgggcgaat tattgcaggc
     5581 actggacaaa aaaatattct ccccctacta catcgcgtta atccgcctcc tgattgtgtt
     5641 cggatgccgg acggtagaac tgaggttatc ggagatcagc gagtgggatt ttaccgaaat
     5701 gctctggacc gttccgaagg agcacagcaa aacgaaggta gccatattcc ggcctatacc
     5761 ggaagcaata ctgccgttca tcacgcagct ggtggagcag aacaggcaca cgggcttatt
     5821 gctgggggaa gtgaaacagg aggccagcgt atcgcagtat ggaagattag cgcacaggag
     5881 gcttaatcac cctcactggt cactgcatga catccggcgc acctttacaa ccatgctgaa
     5941 cgatttaggc gttgatccgc atgtcgtgga gcagcttaca ggccaccaga tgccaggaat
     6001 gcagcgagtt tataatcatt cccgttatct ggatgcgaaa cgcaatgcgc tggatatgtg
     6061 gacggagcgg ttagggatac tggcgggaac acatgaaaac gtaaccacgc taccagtagc
     6121 cagaagaaaa taattttttt cgtgtttttt cagtatgcgc atactggata tgcgaacaga
     6181 tacaacgtgc aacaatgagc aacaatgtgg aacaactacg aacaagaggc gaaaaagcgt
     6241 acggatttat aagtagctga tttttaatgt gttactggtt ttttatacac tagcgaatca
     6301 ctctttaaag agcgagaaaa aaggcatgga a
//
LOCUS       DAZGXS010000198         6242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGXS010000198 DAZGXS010000000
VERSION     DAZGXS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            387..1100
                     /gene="aphA"
                     /locus_tag="ACHKG5_004471"
     CDS             387..1100
                     /gene="aphA"
                     /locus_tag="ACHKG5_004471"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0870440.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1211..1627
                     /locus_tag="ACHKG5_004472"
     CDS             1211..1627
                     /locus_tag="ACHKG5_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0870441.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1631..1987
                     /locus_tag="ACHKG5_004473"
     CDS             1631..1987
                     /locus_tag="ACHKG5_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0870442.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2022..4844)
                     /gene="uvrA"
                     /locus_tag="ACHKG5_004474"
     CDS             complement(2022..4844)
                     /gene="uvrA"
                     /locus_tag="ACHKG5_004474"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0870443.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5099..5635
                     /gene="ssb1"
                     /locus_tag="ACHKG5_004475"
     CDS             5099..5635
                     /gene="ssb1"
                     /locus_tag="ACHKG5_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0870444.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5734..6015)
                     /locus_tag="ACHKG5_004476"
     CDS             complement(5734..6015)
                     /locus_tag="ACHKG5_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0870445.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         1520 a         1616 c         1614 g         1492 t
ORIGIN      
        1 cgggagtaat aaaattaatc accattgtag ggtagggttc tggtcaatca gaaatcatct
       61 ttataaactt cgattgtttt tgtaatgctg tatcattaag ttcattaaat cgtacagcag
      121 ataaatgttc tatcaaattt cgctcatttg cagagtattc atcataataa acgtaaaatt
      181 aatgtatcct tacatcgagc attaaccatt ttttacaaaa aaacgacagg aacgtattta
      241 ctaggttaaa tataatcatc ctgcttttca tcacaaaaac cgcagataat ccttcctttc
      301 ccctgtagct ggcgttatgg tcagatagtt tttgcaacaa atctcacaat aaaaagtttc
      361 aacatactga ctatttaggg aaaaatatgc gcaagatcac acaggcaatc agtgccgttt
      421 gcttattgtt cgctctaaac agttccgctg ttgccctggc ctcatctcct tcaccgctta
      481 accctgggac taacgttgcc aggcttgctg aacaggcacc aattcattgg gtttcggtcg
      541 cacaaattga aaatagcctc gcagggcgtc cgccaatggc ggtggggttt gatatcgatg
      601 acacggtact tttttccagt ccgggcttct ggcgcggcaa aaaaaacttc tcgccagaaa
      661 gcgaagatta tctgaaaaac cctgtattct gggaaaaaat gaacaacggc tgggatgaat
      721 tcagcattcc aaaagaggtc gctcgccagc tgattgatat gcatgtacgc cgtggtgacg
      781 cgatcttctt tgtgactggt cgtagcccga cgaaaacaga aacggtttca aaaacgctgg
      841 cggataattt tcatattcct gccaccagca tgaatccggt gatctttgcg ggcgataaac
      901 cagggcaaaa tacaaaatcg caatggctgc aggataaaaa tatccgaatt ttttatggcg
      961 attccgataa cgatattacc gccgcacgcg atgtcggcgc tcgtggtatc cgcattctgc
     1021 gtgcctccaa ctctacctac aaacccttgc cacaagcggg tgcgtttggt gaagaggtga
     1081 tcgtcaattc agaatactga cagagcggga gagcgtgaca ctctcccgaa tgctgttttt
     1141 ttaatcacac ctttatcctt tcgctgtctt gctgcaaact gattaagaga gttttatcaa
     1201 ggagcagcac atgtggtatc aaaagacgct cacgcttagc gccaaatctc gtgggtttca
     1261 tctggtaacg gatgaaattc tgaatcagct ggctgatatg ccgcgcgtta acatcggctt
     1321 actgcatctg ttgctgcaac atacctccgc ctctctgaca cttaatgaga actgcgatcc
     1381 caccgtacgc cacgacatgg agcgtttttt cctccgcacc gttcccgaca acggaaatta
     1441 tgagcatgac tatgagggag cagacgatat gccttctcat atcaaatcct caatgctagg
     1501 aacatcgctt gtattgccgg tgcataaagg gcgtattcag accggcacct ggcaaggcat
     1561 ttggctgggg gaacatcgca tccacggcgg atcgcgtcgc atcatcgcga cactacaagg
     1621 ggagtaaaaa atgaccattt cggagttgct acaatattgc atggcaaaac caggcgcaga
     1681 acagagcgtg cataacgact ggaaagcgac gcagatcaaa gtggaagatg tactgtttgc
     1741 gatggtgaaa gaagtagaaa atcgcccagc tgtttcgctg aaaaccagcc cggagctggc
     1801 ggagctgcta cgtcagcagc acagcgatgt gcgtccaagc cgccatctga ataaagcgca
     1861 ctggagcacc gtgtatctcg acggttcgct gccggattcg caaatctatt atctggtgga
     1921 tgcgtcttat cagcaggcgg tgaatttact gccggaagaa aaacgtaaat tgctggtgca
     1981 actctgaaag gaaaaggccg ctcggaaggc ggccttaacg attacagcat cggcttgagg
     2041 aagcgtgccg tatgcgaagc ttcgcactcc gcgacggttt ctggcgtacc ggagacgagg
     2101 atctcgccgc cgccactgcc gccttccggc cccaggtcga caatccagtc agcggtttta
     2161 atcacgtcga gattgtgctc aatcaccaca atggtgttgc cctgatcgcg cagtttatgc
     2221 agtacgtcga gcagttgctg aatatcggcg aagtgcagac cggtggtcgg ctcgtcgaga
     2281 atatacagcg tctgcccggt gccgcgtttt gacagctcac gcgccagctt cacgcgctgg
     2341 gcttcaccac cagaaagtgt ggttgcggac tgccccaagc gaatgtacgt cagaccaacg
     2401 tccatcaacg tttgcagctt acgcgccaga gctggcaccg catcaaagaa ctcacgcgcc
     2461 tcttcgatgg tcatatccag cacttcgtgg atggttttgc ctttgtactt aatctccagc
     2521 gtttcacggt tatagcgttt acctttgcac tggtcgcacg gcacgtaaat gtccggcagg
     2581 aagtgcatct ccactttgat cacgccatcg ccctgacagg cctcgcagcg tccgccacgg
     2641 acgttaaagc tgaaacgtcc tggcgtataa ccacgcgcac gggattccgg tacgcccgca
     2701 aacagttcgc gcacaggcgt aaacacgccg gtataggtcg ccgggttaga acgcggagta
     2761 cgaccaattg ggctttggtc gatatcgatt actttgtcga aatgctccag tccctgaata
     2821 tcgcgatacg gtgccggttc ggcgatggtc gcaccattca actggcgttg ggcaatcggg
     2881 aacagtgtgt cgttaatcag cgtcgattta ccggaacctg aaacccctgt gatgcaggta
     2941 aacagaccga ctggcagcgt tagcgtcacg tctttcaggt tgttgccgcg tgcgccagtc
     3001 agcttaagta ctttttccgg attcgccgga acgcgtttct tcggcacttc aatcttgcgt
     3061 ttaccgctca tgtactgtcc ggtcaacgac tccggcaccg ccataatcgc ttccagcgga
     3121 ccttccgcga ccacttcacc gccatgtaca cctgcaccag gaccgatatc gatcacatga
     3181 tcagcggcgc gaatcgcgtc ttcgtcgtgc tccaccacaa tcacggtatt accgagatcg
     3241 cgcagatgga taagcgtacc caacaggcgc tcgttatcgc gctggtgcag gccgatagac
     3301 ggctcatcca gcacgtacat aacgccaacc aggcccgcac caatctggct cgccagacgg
     3361 atacgctggg cttcaccgcc ggaaagcgtt tctgccgagc gggaaagcgt caggtaattc
     3421 aggccgacgt taacgaggaa tttcaaacga tcgccgatct ctttaagaat tttttccgca
     3481 atcttcgccc gctgacctgc gagtttgaga ttgttgaaga attccatcgc atgaccaatg
     3541 ctcatgtcgg agatggcggg cagcggcgta ttctcgacat acacgtggcg cgcttcccga
     3601 cgcagacgcg tcccttcgca gctggcgcac gggcgattgc tgatgaactt ggctaattct
     3661 tcacgtaccg cactggattc tgtctcttta taacggcgct ccatattgtg cagcacgcct
     3721 tcgaacggat gacgacggat ggaggtatcg ccacgatcgt tcatgtattt gaattcaatg
     3781 ttttctttgc cagaaccgta caacaccact ttatgcacgt tcgcgctcag gctgccccac
     3841 ggcgcttcga cgtcgaactt atagtgatct gccagcgatt tcagcatctg gaaataatag
     3901 aagttgcggc gatcccagcc acggatcgca ccacctgcca gcgacagttc cggattctgg
     3961 atcacgcggt caggatcgaa atattgctgt acgccaaggc cgtcgcaggt cgggcaggca
     4021 cctgccgggt tgttaaacga aaacagtcgc ggctccagtt cacgcatact gtagccgcaa
     4081 attgggcagg cgaagttggc ggaaaacagc agctcttccg ctttcgggtc gtccatatcc
     4141 gccactaccg cggtaccacc ggaaagctcc agcgcggttt caaacgactc ggcaagacgt
     4201 tgggtaagat cgtcacgcac cttgaagcga tcaaccacca cttcaatagt gtgtttcttt
     4261 tgcagttcca gtttcggcgg atcggaaaga tcgcagactt cgccatcaat acgagcacgg
     4321 atgtaaccct ggcttgccag gttctccagc gttttggtgt gttcgccttt gcgctcttta
     4381 atgattggcg cgagcagcat cagacgcttg ccttccggct gcgacagcac gttatccacc
     4441 atctggctga cggtttgcgc cgccagcggg acgtcgtggt ccggacagcg cggttcgccg
     4501 acgcgggcgt acaacaaacg caaatagtcg tggatttcgg tgattgtccc caccgtagaa
     4561 cgcgggttat gagacgtcga tttctgctca attgagatgg caggagaaag cccctcaata
     4621 tgatcgacgt ccggcttttc catcagtgac agaaactgcc gcgcgtaggc ggaaagggat
     4681 tcaacgtaac ggcgctgccc ttcggcatat aaggtgtcga aagcgagcga ggatttgcca
     4741 gaacccgaaa gcccggtcac gacaatgagc ttgtcgcggg ggataacgag gttgatgttt
     4801 ttgagattat gggtgcgggc gccccgaact tcgatcttat ccattcacct ttcccggatt
     4861 aaacgctttt ttgcccggtg gcatggtgct accggcgatc acaaacggtt aattatgaca
     4921 caaattgacc tgaatgaata tacagtattg gaatgcatta cccggagtgt tgtgtaacaa
     4981 tgtctggcca ggtttgtttc ccggaaccga ggtcacaaca tagtaaaagc gctattggta
     5041 atggtacaat cgcgcgttta cacttattca gaacgatttt ttttcaggag acacgaacat
     5101 ggccagcaga ggcgtaaaca aggttattct cgttggtaat ctgggtcagg acccggaagt
     5161 acgctacatg ccaaatggtg gcgcagttgc caacattacg ctggctactt ccgaatcctg
     5221 gcgtgataaa gcgaccggcg agatgaaaga gcagactgaa tggcaccgcg ttgtgctgtt
     5281 cggcaaactg gcagaagtgg ccagcgaata tctgcgtaaa ggttctcagg tttatatcga
     5341 aggtcagctg cgtacccgta aatggaccga tcaatccggt caggatcgct acaccacaga
     5401 agtcgtggtg aacgttggcg gcaccatgca gatgctgggt ggtcgtcagg gtggtggcgc
     5461 tcctgcaggt ggcaatatcg gtggtggtca gccgcagggc ggttggggtc agcctcagca
     5521 gccgcagggt ggcaatcagt tcagcggcgg cgcgcagtct cgcccgcagc agtccgctcc
     5581 ggcagcgccg tctaacgagc cgccgatgga ctttgatgac gatattccgt tctgatttgt
     5641 cattaaaaca ataggttata ttgttttaag gtggatgatt aaagcatctg ccagccataa
     5701 aaaagaagcc tccgttatgg aggcttctac gtatcaggtc aaaatcattg gccactgtgg
     5761 cggtgtctga ttcatctctt ctatcatcac cgactcgatg ttgtgccaga tggcggtgat
     5821 gtccgccatt gtgatgccaa gcaaccccat tgcgaaccag caggcggaaa ccacgcccag
     5881 accgctgctg atcactgcaa gcccgacata atcagaccga cgaaaacgga tgttgagcgt
     5941 gctggccaga aacatcatta cggcactaag aagttgccag cgaagaagaa ccacgccagt
     6001 ggtgagggta gccatcaaac aattcctctg aaaagagccg atgccctgga cagcgcggcg
     6061 ttgttcacgg gaggcgtggt acactctggc tatcgcgggg cttgcagaac acaaaaatga
     6121 aacactctgt ttgtttcatt aattttgtga actatatcac aattgattgt ttgttagcca
     6181 gattaggtcg tgacttttat tgctgtacag attatgtggt ttttcagtgg cattaagggt
     6241 at
//
LOCUS       DAZGXS010000199         6160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGXS010000199 DAZGXS010000000
VERSION     DAZGXS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(23..556)
                     /locus_tag="ACHKG5_004477"
                     /pseudo
     CDS             complement(23..556)
                     /locus_tag="ACHKG5_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(561..725)
                     /locus_tag="ACHKG5_004478"
     CDS             complement(561..725)
                     /locus_tag="ACHKG5_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870446.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(722..1402)
                     /locus_tag="ACHKG5_004479"
     CDS             complement(722..1402)
                     /locus_tag="ACHKG5_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0870447.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1490..1951)
                     /locus_tag="ACHKG5_004480"
     CDS             complement(1490..1951)
                     /locus_tag="ACHKG5_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0870448.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1944..2984)
                     /locus_tag="ACHKG5_004481"
     CDS             complement(1944..2984)
                     /locus_tag="ACHKG5_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0870449.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2956..3507)
                     /locus_tag="ACHKG5_004482"
     CDS             complement(2956..3507)
                     /locus_tag="ACHKG5_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0870450.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3491..3718)
                     /locus_tag="ACHKG5_004483"
     CDS             complement(3491..3718)
                     /locus_tag="ACHKG5_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0870451.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3795..4202
                     /locus_tag="ACHKG5_004484"
     CDS             3795..4202
                     /locus_tag="ACHKG5_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870452.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4466..4765
                     /locus_tag="ACHKG5_004485"
     CDS             4466..4765
                     /locus_tag="ACHKG5_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870453.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4838..5056
                     /gene="ydfC"
                     /locus_tag="ACHKG5_004486"
     CDS             4838..5056
                     /gene="ydfC"
                     /locus_tag="ACHKG5_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP0870454.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5079..5486
                     /locus_tag="ACHKG5_004487"
     CDS             5079..5486
                     /locus_tag="ACHKG5_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870455.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5464..5697)
                     /locus_tag="ACHKG5_004488"
     CDS             complement(5464..5697)
                     /locus_tag="ACHKG5_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870456.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1524 a         1601 c         1366 g         1669 t
ORIGIN      
        1 cgcatattga agagtataaa ccttacatgt tgattacatt tttgtaatca acatcctgtt
       61 tggaatagcc agcctttaat ggataactat ttctgacaat gcaatgagta taatcaagtc
      121 catcttccac tgagaattag aggcggcatg ctttttccgg ctcttgccgg atatccgtaa
      181 ttgtccataa tctgcagatt tataccttct gcatgacctg tcagcgaaaa tttgtccggt
      241 gtttctacgg gaatgacatc aaaagttaca cgcactcgcg ttaccgtgta gacctacttt
      301 cctgcacttg caagatcaca gtggtgtaac cgtaacagga atttattctc tggaccggca
      361 gtaaatccct gagtggcgtg gttcccatat caatttcccg ccaggcagcc tccattgcca
      421 gcgtacaggc tggagccatg acctgccctt taaatctggc ccgaccatcc caccggacgt
      481 gttcttctcc cctgaactta ggtacagtca tctccagtgg cacaaaagtg tcagcgccat
      541 aatttttgac cgttatcgcg ctacggatat tttgttgact ggtgaaaatc accccgcaga
      601 atcaggctta ttcccttaac ctgggctttc atcctgaccg ccgcctcact acgaccaatc
      661 agactgccga tgcattttac cttcattgtt agtatcatga tttcaggcct gcaccatccg
      721 ctcattgccc ggacttccga caaatcccgg caaccatatc ccggtgcttg ttcagctccc
      781 gcagcgcggc gcagactcgc tcccacttct ggacatgatt cttcgcccta cgcagttcgc
      841 gatttgccat atgcagcgat ggcaaaatca aatcatcttc tcgcgttgca gtaaacgatg
      901 gcagcgactg cacaatgtcc gccacagttt ctgttttaat atcttcctgt gttgcatctt
      961 tctgtaccgg taacgcaaca ccggctggct gaggaaaggc tttaccatcg gtttccgcta
     1021 ccgattcaac tttcggctct gctggtaaat tatcacctgg tatgcagtaa cgaattttac
     1081 cgttctggtt tacgcgaatc agacgacctt tgctgattgc cattgccagc gttgaagcaa
     1141 ctttgcggga tgttgtaccg aacagcgtag ccagttcatc cgccgtttgt ggtccgcgtt
     1201 gttcaatcgt cgcggttaaa tcgcactctg agattttcgc ttctgttgcc gtggtggttt
     1261 cttctggcgc tggctgttcc tgctgaacgt tgttatcagc cacacgccag gtgtatacgc
     1321 ttttatcaac gaagccagcc tttttcagtt cccacagctc gttcagtact tcttcacgac
     1381 tgatatcaag tcgcgcagcc agctataccg acgtggcttt tcccatcgct ttcagtgcgt
     1441 caaaaacagt ctccataaat ttcctcccgg taaaaaatca cttctcaact caaacaaaac
     1501 cagccgcttt ccggcgttca tattcctgtt tcagcaactc aattggcgtt ggccccgacg
     1561 ggcgtttggg ggccgccagt tgtcgccgga ctggcggaac gctcaggccg ttactaacat
     1621 gctttgccca tttcgtcagt tgccgttctg caagccgttt taattcccct tcggtcatct
     1681 ggcgctcaat cccctttgaa cgcatctcga ggcaaatgtg atacagcaca ggctgagacc
     1741 acgggtactt atcacttccg tcgtatcgcc aggactcatt gcgccagcgg cggtactcct
     1801 ccatcacagc atccaccgtc agaccgaatg gattggctcc gcttttcgaa atcagtgcca
     1861 caaactcagc caggtccgga ggccatgttt cacccgcccg gcagcggtcc atgcactggc
     1921 ggcagacctg ccggatttgc tgctcagtca tcgcgccaat ctgtgcaatc cagagcttcg
     1981 aaggtgcggc cccgttcttc tggatccagc ggttcgaata aaccaccccc atgagttccc
     2041 acagcttcca ggccgtttcc gtcgctgata aatccgtttt cacgttccca ctgctcacgt
     2101 gctgcccgaa tttcctgaac tgcccgtgat gcggtgccac ctggtgctgc tgcatggttt
     2161 acccctttgc tgactggttt aacctgcgcc ctgacgtgat ttacgtgacg ggcgaatttc
     2221 tgctcccact gaatctgcgt aaacactttc ccctccgctg cccagtagtc ccggaaggcg
     2281 gcaagttcag caggtgtaaa ttctgtctcc ggcaaagcca tcccccacaa cgcagcccgt
     2341 cgtcgaaaat cccgtgacgg ataccagcta tcgctcatcg ggaattttcc gatgggttcg
     2401 ctcaggccat acaggaatac agggggggct gcctgtaacg acaaaacttc ctgctcactg
     2461 gtcggagcac tctcgcgtgc gttatgtgtg gggtttagat ctttgggttc ctttgggttc
     2521 cgtgatccgt ttttgggtgt ctttgatgga aaatttgggt gtctttggtt attttccatg
     2581 cagctaagag ttctgttttt gggtctgttt tgtgctgaaa cataaccatt ttcggtactg
     2641 tttttattaa cagcaccaat tttacccacc tttaaagact cccgtttttg ggtgtattca
     2701 ggctcggcaa cactttcttc tacaccgata agtcggtaca ccacaatttg ctttgttctg
     2761 ccttttctct caccggtatc aacaattaac ccaatctcca tcaggtgtcg taagctgtcc
     2821 tgcacagtct ttttgttcag ttccgttact tctgccagtg cagatacaga cgggtatgca
     2881 cacaaatcgg caccgcacat atcagcaagc caggtcaata ctgacttact ggatgaactg
     2941 ccggttttca cctttttagc ccatcgtagt gcatcgatac tcatacgccc ccctggcaga
     3001 catttgttta tctgcaaagt aatattggta ttgctgacga tacgcgtgct tgaaagcaat
     3061 agctttttct ataagctcgt cagtctcacg ttccacaaca actggatccg caaaaagcag
     3121 cccggactcc accacatcgc catattcttt gtttaacccg gcgatcatgt acgtaatgct
     3181 ttttccgtca gtaatttcac aatacaatct gaaatcgctg atccggatag cctccataat
     3241 tgccggaatc agcgccgtga atttgtcacg cttatctctg gtgtcgatag ctttccagcg
     3301 ttcgaatatc ttcacccggt taacgcccag cgcccgttga tcaacctcgc catcattaaa
     3361 cgtgacgcgt tgaacatcga tgttcgggcg ttctttcaga gcccagaatg cttccgtgat
     3421 taatatcgtc gcctgctcct gtgtcattcc tggtcggcat acccaggtat ccagagcctc
     3481 acaaacctgt tcagtggtga ttttcattgt tcaaccgccc cgcccgcttt gccttacgat
     3541 attcgtcata aactttgggg tcgtactgaa gttccccgcc ggatgcctct tgcaggcgca
     3601 tcgcgcgacc ttcaggaacc agttcccccc atgcagcaac gcttgccagc ctaactcctg
     3661 cgacattggc aagctttgtt ttgctgccaa aaaacgctat agcatcaatt ttcaacatat
     3721 cgaacccctt agattttcct aaggaaacta gatcgtagag aaacctaagt caagaaaaat
     3781 tagaattacc taatatgaaa aacgaaacct tcggtgctcg cctcttatac aggcgaaaaa
     3841 aattaaaact gtctcaggcc gcattaggta agctggtcaa agtggctcac gtaacaattt
     3901 ctcaatggga aagagatgaa acacagccag cggggaagag attattcgca ctgagccagg
     3961 cgcttcagtg ctcgccgact tggcttcttt ttggagatga agataagcaa ccaggcgaac
     4021 cgatcccaga taatcagcca gtcaatctga cagaagatca aaaagagttg cttcaactgt
     4081 tcgacgcact gcctgagtca gagcaaaagg ctctgttgtc agagatgcgt gctcgagttg
     4141 agaatttcaa caaacttttt gaagaactac tcaaagctcg caaaagaagc gcaaataaat
     4201 aacccccctt tttttcgcaa ctctctgtaa taaaaagcac aaactttcaa atacttgtgt
     4261 tttttacatc aagaagctta ggtttttcta cacaaaaagc ttgaccataa ctcttaggct
     4321 attctaaatt ccactcatca agacaccgca cggtgttctc agcaaacagt tccgctaccc
     4381 ggcgttaagg ggaagcagag gatttctcag tgggcgagtc aaacatcaga atggaaggcg
     4441 tccagggatc agcaaagaaa cagcgatggc gctttatatt gatatcagcg ccattgccgg
     4501 acaggtaaga gttatcagag cggtaactaa gcggtatgcg cctttacttc agaaagtctc
     4561 tggtgagtgc accgaagata ttgtcaacga tttcgtcatc gaactgcgag gactcatctt
     4621 cagttacaag gtgaccacaa tttttgcaga tggctcccgc gaaactgtca gagccctgcg
     4681 gcttaaagga tgtgtcaaag acttagccac cacattctgg gcaagaaaac ttgattgtat
     4741 tcataaccaa tttcctctcg agtaacagac ccctcagagg ataccacctc gcctgacgtg
     4801 gttaaaaagc aggcaacgct aaccacaagg agccgacatg cagaaacgag accccgtcat
     4861 catcgcgcca gactataccg atgatgaact ttatgagtgg atgcaccaga aaattaaggc
     4921 ggtgcaggac ctgaaatggg ccaatgaagc cagggctaag caggctgaaa atctgtccgc
     4981 tctggagcag gatatcacca atctggaaaa agcagcggca ttaagcattg ccagaatgat
     5041 tacataccca cgttaatagc taaccaacga ggctaataat ggaatttaaa gatttaccaa
     5101 cgccattaca ggaaatggca tcgaatatag ttcgttcaca actggctact cttgacctta
     5161 gtaccgcaga aaaagaaacc atcgataata tggttcgtaa tgtgcgcaat gctttttctg
     5221 ggctatatgg ttctgataat caaaagcagg aaagcgatgt taataaacgg gtaatttctg
     5281 tttgcgtgaa tggccatgtt ctttcatcaa tcaaaacaga aacggcgaca gtcttcgatt
     5341 gcctttgcat tgtacagagc cttgttgatg ccctgtttcg ttcagtgaat ttagaaaatg
     5401 atgcaaatct gcgagggcgc ataatagcac atccatatgc acatacttta ggctctgtgg
     5461 atatcaaaga tcccacaaat ctttaatgaa atagttaacg cgaattgtac ttgctctttc
     5521 ggttgctttc agaatacgcg ttgaaactgc tggcggtaat ttggtattcc atttattaaa
     5581 atcatgcccg ggaaagtact cttcgaaaat acttttaact gcagactcgc ctattgaaat
     5641 gctgcttacc atgcgatttt gataaaggca tttagcaata agagttgatt ttaacattca
     5701 ccctcctgag ggttggtaat taaggagttc tccacgggtg aagtggagtg cgtgcgccgg
     5761 acacgggtga acatccggca ctgacagttt actgaaagga tatttctctg aaaagtcaga
     5821 gcataacgcg aaagcgcacg gcgaggttgc tggttcatag atagcctgtc gttaaatttt
     5881 cgtcgaccgt gcgcttccgg ttgtggcact ccgcgaaatg gcgcagcggt aagtatggcg
     5941 gggttattcc ttccccgttg aggacaccgg gttgtcaggt tgaccatacg cttaagtgac
     6001 aaccccgctg caacgccctc tgttatcaat tttctggtga cgtttggcgg catctgtttg
     6061 cccatgaact gatgtccgcc ctttttaaag tgaattttgt gatgcagtga atgcggctaa
     6121 gcgcacgcgg aacagttaaa gctaaaaaca gtgttatggg
//
LOCUS       DAZGXS010000200         6046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGXS010000200 DAZGXS010000000
VERSION     DAZGXS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            211..1314
                     /gene="ompC"
                     /locus_tag="ACHKG5_004489"
     CDS             211..1314
                     /gene="ompC"
                     /locus_tag="ACHKG5_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0870457.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1426..2481
                     /gene="apbE"
                     /locus_tag="ACHKG5_004490"
     CDS             1426..2481
                     /gene="apbE"
                     /locus_tag="ACHKG5_004490"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0870458.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2555..3619
                     /gene="ada"
                     /locus_tag="ACHKG5_004491"
     CDS             2555..3619
                     /gene="ada"
                     /locus_tag="ACHKG5_004491"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0870459.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3619..4269
                     /gene="alkB"
                     /locus_tag="ACHKG5_004492"
     CDS             3619..4269
                     /gene="alkB"
                     /locus_tag="ACHKG5_004492"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0870460.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4345..5988
                     /gene="yojI"
                     /locus_tag="ACHKG5_004493"
     CDS             4345..5988
                     /gene="yojI"
                     /locus_tag="ACHKG5_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0870461.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1440 a         1528 c         1635 g         1443 t
ORIGIN      
        1 ttccgcaata atcttaaaaa gttccctcga atttacattt tgaaacatct atagcgataa
       61 atgaaacatc ttaaaagttt tagtatcata ttcgtgttgg attattctgc atttttgggg
      121 agaatggact tgccgactga ttaatgaggg ttaatcagta tgcagtggca taaaaaagca
      181 aataaaggca tataacagag ggttaataac atgaaagtta aagtactgtc cctcctggtc
      241 ccagctctgc tggtagcagg cgcagcaaac gctgctgaag tttacaacaa agacggcaac
      301 aaattagatc tgtacggtaa agtagacggc ctgcactatt tctctgacga caagtctgta
      361 gatggcgacc agacctacat gcgtcttggc ttcaaaggtg aaactcaggt tactgaccag
      421 ctgaccggtt acggccagtg ggaatatcag atccagggca acagcgctga aaacgaaaac
      481 aactcctgga cccgtgtggc attcgcaggt ctgaaattcc aggatgtagg ttctttcgac
      541 tacggtcgta actacggcgt agtttacgac gtaacttcct ggaccgacgt tctgccagaa
      601 ttcggtggtg acacctacgg ttctgacaac ttcatgcagc agcgtggtaa cggtttcgcg
      661 acctaccgta acactgactt cttcggtctg gttgacggcc tgaactttgc tgttcagtac
      721 cagggcaaaa acggtagcgt aagcggcgaa ggcatgacta acaacggtcg tgaagcactg
      781 cgtcagaacg gcgacggcgt tggcggatct atcacttatg attacgaagg cttcggtatc
      841 ggtgctgcag tttccagctc caaacgtact gatgatcaga acagcccgct gtacatcggt
      901 aacggcgacc gtgctgaaac ctacactggt ggtctgaaat acgacgctaa caacatctat
      961 ctggctgctc agtacaccca gacctacaac gcaactcgcg taggttccct gggttgggcg
     1021 aacaaagcac agaacttcga agctgttgct cagtaccagt tcgacttcgg tctgcgtccg
     1081 tccctggctt acctgcagtc taaaggtaaa aacctgggtg tcatcaatgg tcgtaactac
     1141 gacgacgaag atatcctgaa atatgttgat gttggcgcga cctactactt caacaaaaac
     1201 atgtccacct atgttgacta caaaatcaac ctgctggacg acaaccagtt cactcgtgac
     1261 gctggcatca acactgataa catcgtagct ctgggtctgg tttaccagtt ctaatctcga
     1321 ttgatatcga acaaagggcc tgcgggccct tttttcattg ttttcagcgt acaaactcag
     1381 ttttttggtg tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt
     1441 acccgcgtgg cactgctggc tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa
     1501 cccgccaaaa cccacgctac tgaagttacc gttcttgaag gcaaaactat gggtaccttc
     1561 tggcgtgcca gcatcccggg cattgacgcc aaacgcagcg ccgaacttaa agaaaagatt
     1621 cagacccagc tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg
     1681 atgcgcttta acgactcgca aagtttatcg ccgtggccgg taagtgaagc gatggccgat
     1741 atcgtcacca cctcgctacg cattggcgcg aagaccgatg gcgcgatgga tataaccgtc
     1801 gggccgctgg tgaatctgtg gggtttcggt ccggaacaac agccggttca aattccgagc
     1861 caggaacaga tcgacgcgat gaaagccaaa actggtttac agcaccttac ggtcattaat
     1921 cagtcgcatc agcaatatct gcaaaaagac ctgccggatt tatatgtcga tctctccacc
     1981 gtcggcgaag gttatgcggc ggatcacctg gcacgcttga tggagcagga agggatttcc
     2041 cgctatctgg tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgaaggcctg
     2101 ccgtggcggg tagcgattca aaaaccaacc gataaagaaa acgcggttca ggccgtggtg
     2161 gatatcaacg gtcatgggat tagcacctct ggcagctacc gtaactatta cgaactggac
     2221 ggcaaacgtc tttcccatgt tatcgatccg caaaccgggc gtcccatcga acacaatctg
     2281 gtatccgtga cggtgattgc accgacggcg ctggaagccg acgcctggga tacaggtttg
     2341 atggtactcg ggccggagaa agccaaagaa gttgttcgcc gggaagggct ggcggtttat
     2401 atgatcacca aagaaggcga tagctttaaa acctggatgt caccacagtt taaaagcttc
     2461 cttgtcagcg aaaaaaatta aagcgcaaga ttgttggttt ttgcgtgatg gtggccgggc
     2521 agactaaagg ctatccttaa ccagggagct gattatgaaa aacgccacat gcttaactga
     2581 cgatcaacgc tggcaatctg tcttagcccg cgacccgaat gccgacggcg aattcgtttt
     2641 cgccgtgcgc accacaggca tcttttgccg tccgtcttgc cgcgccagac atgctttgcg
     2701 ggaaaacgtc tccttctacg caaatgccag cgaggcactc gccgccggat ttcgcccctg
     2761 caaacgctgc caacctgata aagccaatgc ccagcaacat cggttggata aaatcaccca
     2821 cgcgtgtcga ctgctggaac aggaaacgcc tgtaacgttg gaagccttag ccgaccaggt
     2881 ggcgatgagt ccatttcatc tacatcggtt gtttaaagcg actaccggaa tgacgcctaa
     2941 agcctggcaa caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag
     3001 cgtgacgacg tctattctta acgccggatt ccccgacagc agcagttact accacaaagc
     3061 cgatgaaacg ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt
     3121 gcgttacgcg ctggctgatt gtgagctggg tcgttgcctg gtggcagaaa gcgagcgggg
     3181 gatttgcgcg atattgctgg gcgatgatga cgcgacatta atcagcgagt tgcagcagat
     3241 gtttcccgct gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt
     3301 gatcgccagc ctcaatcaac gcgatactcc gctgacgtta ccgctggata ttcgcggcac
     3361 tgcttttcag caacaagtct ggcaggcact gcgcacgata ccttgcggtg aaaccgtcag
     3421 ttatcagcaa ctggctaacg ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc
     3481 ctgtgccgcc aacaagctgg ctatcgtaat tccctgtcat cgagtggtcc gtggtgatgg
     3541 cacactttcc ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc
     3601 tgaaaatgag gagaggtaat gttggatctg tttgccgatg ctgaaccgtg gcaagagcca
     3661 ctggcggctg gtgcggtaat tttacggcgt tttgctttta acgctgcaga gcaactaatc
     3721 cgcgatatta atgacgttgc cagccagtcg ccgtttcgcc agatggtcac ccccggggga
     3781 tataccatgt cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa
     3841 ggttatctct attcgcccat tgatccgcaa acaaataaac cgtggcccgc catgccgcag
     3901 agttttcata atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca
     3961 gatgcttgtc ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa
     4021 gacgaaccgg atctgcgcgc gccaattgtt tctgtttctc tgggcttacc cgcgattttt
     4081 caatttggtg gcctgaaacg taatgatccg ctcaaacgtt tgttgttgga acatggcgat
     4141 gtggtggtat ggggcggtga atcgcggctg ttttatcacg gtattcaacc gttgaaagcg
     4201 gggtttcatc cactcaccac cgactgccgc tacaacctga cattccgtca ggcaggtaaa
     4261 aaagaataaa aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg
     4321 taattcattg tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc
     4381 tggccattta tcagtgtgat ggcgctaagc ctcgccagtg cggctttagg cattggctta
     4441 attgctttta tcaatcagcg ccttatcgaa acggcggata ccagtctgct ggtgttgccg
     4501 gagtttctgg gattattgct gctgttgatg gcagtcactc tcggatcgca actggcgctc
     4561 accactttgg ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctg
     4621 gatactcacg tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc gggattaacc
     4681 agcgatgtgc gcaatatcac cattgctttt gtgcgtctgc cggaactggt gcaggggatc
     4741 attctcacta tcggttcagc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta
     4801 acggctatct ggatggcagt caccatctgg ggcggttttg tgctggtggc gcgggtgtac
     4861 aaacatatgg cgaccctgcg tgaaaccgaa gacaagctgt acacggattt tcaaactgta
     4921 cttgaagggc gcaaagagct gactctgaac cgggaacgcg ccgagtatgt gtttaacaac
     4981 ctctacattc ctgatgcgca agagtatcgc catcatatta ttcgcgcaga caccttccat
     5041 cttagtgccg tgaactggtc aaacatcatg atgctgggcg ctatcggcct ggtgttctgg
     5101 atggcgaaca gtctcggttg ggctgatacc aacgttgccg cgacctattc gttgacgctt
     5161 ttattcctgc gtacgccgct gctttcggcg gttggcgcat tgccgacgct gctgacggcg
     5221 caggtggcgt ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg
     5281 cgcccgcagg cgtttcccag ctggcaaacg ctggagctgc gtaacgtgac gtttgcttat
     5341 caggataacg cgttttccgt tggtccgatt aatctcacca tcaaacgtgg cgagctgctg
     5401 tttctgattg gcggcaacgg tagcggaaaa tcgacgctgg cgatgttgtt gacgggcttg
     5461 tatcagccac aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg
     5521 gaagattatc gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg
     5581 ctggggccgg agggtaaacc cgctaacccg caactggttg agaagtggct ggcgcagctg
     5641 aaaatggctc ataagcttga gttaagaaac gggcgtattg ttaacctgaa gttatcaaaa
     5701 gggcagaaaa aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg
     5761 ctggatgaat gggcggcgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg
     5821 ctgccgctga tgcaggagat gggtaaaact attttcgcca tcagtcatga tgatcattac
     5881 tttatccacg ccgaccgcct gctggaaatg cgcaacgggc aacttagcga gctgacgggc
     5941 gaagagcgcg atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct
     6001 gacctggcgg actgctgccg ccaggtcaat atgctaaaaa tccctt
//
LOCUS       DAZGXS010000201         6024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGXS010000201 DAZGXS010000000
VERSION     DAZGXS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            114..842
                     /gene="gadW"
                     /locus_tag="ACHKG5_004494"
     CDS             114..842
                     /gene="gadW"
                     /locus_tag="ACHKG5_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0870462.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1205..4318)
                     /gene="mdtF"
                     /locus_tag="ACHKG5_004495"
     CDS             complement(1205..4318)
                     /gene="mdtF"
                     /locus_tag="ACHKG5_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0870463.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4343..5452)
                     /gene="mdtE"
                     /locus_tag="ACHKG5_004496"
     CDS             complement(4343..5452)
                     /gene="mdtE"
                     /locus_tag="ACHKG5_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0870464.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5560..5838
                     /locus_tag="ACHKG5_004497"
     CDS             5560..5838
                     /locus_tag="ACHKG5_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870465.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5839..>6024)
                     /gene="gadE"
                     /locus_tag="ACHKG5_004498"
     CDS             complement(5839..>6024)
                     /gene="gadE"
                     /locus_tag="ACHKG5_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0870466.1"
                     /translation="CFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKL
                     KLRRMSDIVTLGITSYF"
BASE COUNT         1482 a         1499 c         1513 g         1530 t
ORIGIN      
        1 ataaattttg gtcctaccaa atttggcagt ttttgcgcta agaaacagtc tggcatcatt
       61 tcattagtat actgaaattg aaataatcgc agtatgaaat ataagggata atcatgactc
      121 atgtctgctc ggtgatcctc attcgtcgtt cattcgatat ttatcatgaa cagcaaaaaa
      181 tatcgctgca taacgagagt atcctgctgc tggagaaaaa tttggcagac gattttgcgt
      241 tttgttcacc ggatacgcga cgactggata tcgatgagct gacagtttgc cattacttac
      301 aaaatattcg tcagctacca cgcaatttag ggttacatag caaagaccgt ctgttaatta
      361 accagtcacc ccccatgccg ctggtgacgg cgatttttga tagcttcaat gaatccgggg
      421 taaattcacc gatactgagc aatatgctct acctttcctg tttatcgatg ttttctcata
      481 agaaagaact gatcccctta cttttcaata gcatcagcac tgtttcagga aaagttgaac
      541 gccttattag ctttgatatc gccaaacgtt ggtatctgcg cgatatcgcg gaaagaatgt
      601 ataccagcga gagtctcatc aaaaaaaagt tgcaggatga aaacacctgt ttcagtaaaa
      661 tattactcgc ctccaggatg tcgatggcca gacgattact cgagttacgt caaattcctc
      721 tgcatactat tgcggaaaaa tgtggctata gcagtacgtc gtactttata aacacatttc
      781 gacaatatta tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt accttttcct
      841 gacatatttt gcatttgaat attggtcagg atctcacacc tgcttcatgt gaaactcttc
      901 cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatag
      961 gtattttcag gaggaaaaga ctcggcatgt ttgggattat taagctgaca attcatacga
     1021 ttacgggaat gtgggtcagt attgtgctgt tcaaattgat gacgaatggc tggagtggtt
     1081 tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg
     1141 gtgaatggtt agcaggaaag agtaaggctg aaccttcacg ttcaacctta ctctcattta
     1201 cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa
     1261 caaagtaaat tgccagcact gttgcggcaa acatcccgcc catcacgccg gtacctaccg
     1321 cgttttgcgc gccagaaccg gcaccatgac tgataaccag cggcagcacg ccgagaataa
     1381 aggccagaga ggtcatcagg attgggcgta aacgcatccg cgccgcttcg atgattgcct
     1441 ctatcggcgt tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg
     1501 cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtag acgtcattac
     1561 ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga acaaccaaca
     1621 tcaccgagaa cggaattgac cagctctcat aaagtgcggc gagggcgagg aacaccacga
     1681 ccagtgaaat cgcatacagc gcaggagcct gatttgagga taacgcttcc tgatacgaca
     1741 gtccggtcca tgagtagccg acgcctgccg gaagtttagc gaccaggtct gccataaatt
     1801 tcatggcgtc accggtactt ttcccggccg ccgcttcacc taaaatctcc attgacggga
     1861 tgccgttgta gcgttccagt cgcggtgaac cataggtcca ttcggtagac gagtaggcag
     1921 aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca
     1981 acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga
     2041 agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg
     2101 ccacacccat tgcctcagct ttcgcggcgt tgacgttcac tttgaacatc ggcgtatctt
     2161 ccaggccgtt cgggcgtacc ccggtgacct gattcggtga ttgcgctgcc agtgataaca
     2221 gctcgtttcg tgcctgggtt agtttttcgt gccccaggtt accgttgtcc agcagttcca
     2281 tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga
     2341 cggctttatt gatactgctt agcgcaatca ttgcccgctg aatgatcgcg gtaaccgagt
     2401 tttcctcacc gacacgttca gaccacggct tgagactgat aaacgccaga ccgttgtttt
     2461 gcccctggcc gctgaagcca aagccgccaa cggtaaacac cgactgaaca ttatctttct
     2521 cttttgtaag ataataatcc gtcacctgtt gcagcacttt cgtggtgtta accatggtgg
     2581 caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag
     2641 aggtcggcgt gcgcaggaac agcaccgcca tcccggcgca aatcagcagg tagatcacca
     2701 tgtagcgacc ggtacaacgc aacagtgagc gggtgctatc ggtatagtgc tgagttgatt
     2761 tttcaaacag cgtgttgaag cgtgcgaaca gggcattagg tttgtgaccg ccttccggcg
     2821 cggctttcag aatggtggcg cacagggcag gggtcaggct cattgccaca aatactgaaa
     2881 gcagcatgga ggagatcagc gtgatggaga actggcggta gatctccccg gttgcaccgc
     2941 tcataaaggc catcggcata aacactgcgg aaagaacaac ggcaataccg accagcgcac
     3001 gttggatctg ccccatcgat ttatgcgtcg cttccttcgg tggtagctta tcttccgcaa
     3061 tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac cctatcgcca
     3121 gcaccatccc gaacatagtc aacgtgttga tggtgaaacc gaccgccgac aagatcgcaa
     3181 acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggca cggaaattct
     3241 gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagt gttttgaaaa
     3301 cttcctgaat agaaatttcg ataaacggcg tggtgtcgta aggataaacc gtcttcagac
     3361 ttgccgggaa ataggctgat aagcggttca gttcctcttt gactgcccgc gaggtatcca
     3421 gggcgtttgc tccggtagcc agtttgatgg cgatcccggc agcaggtttg ccgttatagc
     3481 gcgccacggt ggaataatct tccgccccaa gttcgacgcg ggcgacatca cgcagcagca
     3541 cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca
     3601 gacgcgtctg cacaatgatc gaggcgttta gttgctggtc tgccgcctgt ggcatgccac
     3661 ccagttgacc accagaaatc tggttgtttt gcaccttaat ctgggaaata acatcggaag
     3721 gtaccagatt gtatttattg agtttttgcg ggtccagcca gatacgcatg gcgtactcgg
     3781 aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag
     3841 acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaacgccg
     3901 ctaccatcag gatattactg ctcgatttat cgacgctaat cccctgctgc tgcactgctt
     3961 caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt gcgatatcag
     4021 gagatgtccc agtctcgaag gtcagagtga tagaggcatt acccgccgca tcactggttg
     4081 aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg
     4141 agtcttctac cgtttgcgca tcggcacccg gataggtagc gctgacggta atggtcggtg
     4201 gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagacca cctgcaagca
     4261 tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga
     4321 aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt tcctggctgg
     4381 aggaaattgc tcgtgctttg atacccggac gaatgcgttg caaaccggaa acgatcaccc
     4441 gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct ttgctggctt
     4501 caatttcgcg tagcttcacg acatcgtctt tatccagaat gagcgccgtt gctttacctt
     4561 gggcgttgtg ggtgacgcct tcctgcggca ccagtaatac attctggcgg ctaccttcat
     4621 ccactaatgc cgtgacatac atgccaggca gcaagtcacc atttgggttg gggaaaatcg
     4681 cccgtaacgt cacggagccc gtggtttcat caaccgtcgg gtcggagaat ttcagcgtgc
     4741 cggtctggct gtaacgttta ccattttcca gattgagctg tactggcgta ctgccctgaa
     4801 cctgtttgat ttgcccactg gcgacctctt ctttcatgcg taagaaatct tgcaccgact
     4861 gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat
     4921 tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg
     4981 aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg
     5041 tgacattggc ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa
     5101 cgtagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg
     5161 tagagagcgc tttcgccagc gagcctttgg cggagtttag ctcggcctgt aaaggtgcag
     5221 gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt
     5281 taatgataat accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac
     5341 cgggcaattc gctcaacaca ttgaccgacc caggggagag tgtgacgaca ccgacctcag
     5401 gcgtcatggc ggcggtgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc
     5461 agaataacaa cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt
     5521 gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc
     5581 ggcatagtgg ggttgtcagc cagtaaggtg ggatacaggc atagtgatcg acatggtgag
     5641 gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg
     5701 aagggttcta ctggtggata cacataccag gggaataaag tcgttttagc ctctccttta
     5761 gtaatcaccg ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt
     5821 cagttgctta tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat
     5881 acgtcttagt ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga
     5941 aatattgagc gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat
     6001 tttgaactga ttatgtgaaa aaca
//
LOCUS       DAZGXS010000202         6016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGXS010000202 DAZGXS010000000
VERSION     DAZGXS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            39..1109
                     /gene="ygjJ"
                     /locus_tag="ACHKG5_004499"
     CDS             39..1109
                     /gene="ygjJ"
                     /locus_tag="ACHKG5_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP0870467.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            1126..3477
                     /gene="ygjK"
                     /locus_tag="ACHKG5_004500"
     CDS             1126..3477
                     /gene="ygjK"
                     /locus_tag="ACHKG5_004500"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0870468.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            4003..>6016
                     /gene="fadH"
                     /locus_tag="ACHKG5_004501"
     CDS             4003..>6016
                     /gene="fadH"
                     /locus_tag="ACHKG5_004501"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0870469.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         1441 a         1628 c         1692 g         1255 t
ORIGIN      
        1 aaaggctgta ctttttcttt agctcatgga ttaacacaat gaaattaatc actgcaccat
       61 gcagagcatt acttgctctg ccgttttgct acgccttttc tgcggcagga gaagaagcac
      121 gtccggcaga acatgacgac acaaaaaccc ccgcaattac ctcgacatct tctccttcat
      181 ttcgttttta cggcgaatta ggggttggtg gatatatgga tttagagggt gagaataaac
      241 ataaatacag cgacggcacc tatattgaag gtggcctgga gatgaagtac ggctcctggt
      301 tcggcctgat ttacggcgaa ggctggaccg tgcaggccga ccacgacggc aatgcctggg
      361 tgcctgacca tagctggggc ggtttcgaag gtgggattaa ccgtttctat ggcggttatc
      421 gaaccaatga tggcaccgaa atcatgctca gtctgcgtca ggattcctcg ctggatgacc
      481 tgcaatggtg gggcgacttc acacccgatc tgggctacgt cattcccaat acccgcgaca
      541 ttatgaccgc gctgaaggtg cagaacttaa gcggcaactt tcgttatagc gtcaccgcca
      601 cacctgccgg acatcatgac gaaagcaaag catggctaca ttttggcaag tacgatcgct
      661 acgacgacaa atacacctat ccggcaatga tgaacggtta catccagtat gaccttgccg
      721 aaggcatcac ctggatgaat ggtctggaaa ttaccgacgg cacaggacaa ctctatctca
      781 cgggactgct tacccctaac tttgccgctc gcgcctggca ccataccgga cgtgccgacg
      841 ggctggacgt accgggaagt gaaagcggga tgatggtgag cgccatgtat gaagcgttaa
      901 agggcgttta tctctccacc gcttacacct acgccaaaca tcgccctgac cacgctgacg
      961 atgaaaccac ctctttcatg cagtttggta tctggtacga atatggcggc ggacgtttcg
     1021 caacggcttt tgatagccgc ttctacatga aaaatgcctc taacgatccc agcgaccaaa
     1081 tcttcctgat gcaatatttc tactggtaat aaggactgta atttcatgaa aatcaaaact
     1141 attttaacgc cagtaacctg cgctctgctg ataagttttt ccgcccatgc cactaacgcc
     1201 gacaattata aaaacgtgat taaccgtact ggtgcgccgc agtacatgaa ggattacgat
     1261 tacgacgatc accagcgttt taatccgttt ttcgatctcg gagcctggca tggtcatctg
     1321 ttgccagacg gcccgaacac catgggcggc tttccgggcg ttgcgctgct gacggaagaa
     1381 tacatcaact ttatggccag caatttcgac cgcctgaccg tctggcagga cggcaagaaa
     1441 gtcaacttca cgctggaggc atacaatatt cccggcgcgc tggtgcaaaa actgacagca
     1501 aaagatgtgc aggtcgaaat gactctgcgc ttcgccacgc cgcgcacgtc actactggaa
     1561 actaaaatca tcagcaataa accgctggat ctggtgtggg atggcgaact gctggaaaaa
     1621 ctggaagcga aagaagggaa accgctttcc gataaaacca ttgctggcga ataccctgac
     1681 taccagcgca aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg caaagtgcgc
     1741 gccacctggg atctgctgac ctccggtgaa tcagaatatc aggtgcataa atccctgccg
     1801 gtgcagactg aaatcaacgg caatcgcttt accagtaagg cgcatatcaa cggttcgacg
     1861 acgctctata ccacctattc ccatctgctg acctctcagg aagttagcaa agagcaaatg
     1921 cagatccgcg atattctggc gcgtccggcg ttttatctca ccgcctcgca gcaacgctgg
     1981 gaagagtatc tgaagaaagg gttaaccaat ccggatgcga cgccggaaca gacacgcgtc
     2041 gcggtgaaag cgatcgaaac gctcaacggt aactggcgct cgcctggggg tgcggtgaaa
     2101 tataacaccg tcacgccgtc ggtgaccggg cgctggttct ccggcaatca gacctggccg
     2161 tgggatacct ggaagcaggc gtttgcgatg gcgcatttca atccggacat cgccaaagag
     2221 aatatccgcg cggtcttctc ctggcagatc cagcctggcg acagcgtgcg tccgcaggat
     2281 gtgggctttg tccccgacct gatagcgtgg aatcttagcc ccgagcgtgg tggcgatggt
     2341 ggcaactgga acgaacgaaa taccaaaccc agccttgccg cctggtcggt gatggaagtg
     2401 tacaacgtca cccaggataa aacctgggtg gcagagatgt acccgaaact ggtggcctat
     2461 cacgactggt ggttacgtaa ccgcgatcac aacggcaacg gcgtgccgga atatggcgcg
     2521 acccgcgaca aagcccacaa cactgagagc ggcgagatgc tgtttacggt gaagaaaggc
     2581 aacaaagaag agacgcagtc tgggctgaac aactacgccc gcgtggtgga gaaaggccag
     2641 tacgacagtc tggaaattcc ggcacaggtt gctgcatcgt gggaatcggg gcgtgatgat
     2701 gccgccgtct ttgggtttat cgacaaagaa cagctggata aatatgtcgc aagcggcggc
     2761 aaacgtagcg actggacagt gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg
     2821 gggtactcgc tattgcagga gtcggtggat caggccagct atatgtacag cgataaccat
     2881 tatctggcgg agatggcgac gattctcggt aaaccggaag aggctaagcg ctatcgccag
     2941 ttggcacagc agctcgcgga ctacatcaac acctgtatgt tcgacccggc tacgcagtac
     3001 ttctatgacg tgcgtattga agataaaccg ctggcgaacg gctgcgcggg caaaccgatt
     3061 gttgagcgcg gtaaagggcc ggaaggctgg tcgccgctgt ttaacggtgc ggcaacgcag
     3121 gccaatgccg acgcggtggt gaaggtgatg ctcgatccta aagagttcaa tacctttgtt
     3181 ccgctgggaa cggcggcgtt aaccaatccg gcctttggcg ctgatatcta ctggcgcggg
     3241 cgcgtatggg tggatcagtt ctggtttggt ctgaaaggga tggagcgtta cggttatcgc
     3301 gatgatgccc tgaaactggc ggatacgttc ttccggcacg ccaaagggtt aaccgccgat
     3361 ggcccaattc aggaaaatta caacccgctg acaggcgcac agcaaggcgc accaaatttc
     3421 tcctggagtg ccgcacattt ttatatgttg tataacgatt ttttccgtaa gcaataattt
     3481 gcaacggctg gcggatgcgg cgtgaacgcc ttatccgccc tacatattcg ttcccgtagg
     3541 ctggataaga cgcgacaagc gtcgcatccg gcatctgcac ctcgccaccg gcggatgctg
     3601 cgtgaacgcc ttatccgccc tacatgttcg ttcccgtagg ctggataaga cgcgacaagc
     3661 gtcgcatccg gcatctgcac ctcgccactg gcggatgcgg cgtgaacgcc ttatccgccc
     3721 tacatgttcg ttcccgtagg ctggataaga cccgacaagc gtcgcatccg gcgtctgcac
     3781 cgcgccactg gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccagg
     3841 tcggataggg cccggcaagc gtcgcatccg gcgttaccac cgggcgtatt ctttttgaat
     3901 cccatcacaa accccgcact ccccttttcc cttttctccg gcgacggcta aattagaact
     3961 catccgacca cataacaatt attttacata ctggacaatt ttatgagcta cccgtcgctg
     4021 ttcgccccgc tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat gggttcaatg
     4081 cacaccgggc tggaggaata cccggacggt gctgaacggc tggcagcgtt ttatgccgaa
     4141 cgcgcccgtc acggcgtggc gctgattgtt agcggcggca ttgcaccaga tttaacaggc
     4201 gttggcatgg aaggcggcgc aatgctcaat gacgccagcc agatcccaca ccatcgcacc
     4261 attaccgaag cggtacatca ggaaggcggc aaaatagccc tgcaaatttt gcataccggg
     4321 cgctacagct accaaccgca tctggtcgca ccgtccgcat tgcaggcccc catcaaccgt
     4381 ttcgttcctc atgaactcac ccatgaagag atcctgcaac tgatcgacga tttcgcccgc
     4441 tgcgcgcaac tggcacggga ggcaggatac gacggtgtag aggtaatggg ttccgaaggg
     4501 tatttgatca acgaatttct gacgctgcgc accaatcagc gcagtgacca gtggggcggc
     4561 gattaccaca accggatgcg gtttgccgtt gaagtggtga gtgcggtacg cgaacgcgtt
     4621 ggcaacgact tcattattat ctaccgactg tcgatgctcg acctggtcga aggcggcggg
     4681 acttttgccg aaacggtaga gctggcgcag gccattgaag cggcgggcgc gaccattatc
     4741 aacaccggca ttggctggca tgaagcgcgt attccgacca tcgccacgcc cgtgccacgc
     4801 ggcgcattta gctgggtcac gcgcaaactg aaaggtcacg tctcgctgcc gctggtgacc
     4861 accaaccgga ttaacgatcc gcaggttgcc gacgatattc tctcgcgcgg cgatgccgat
     4921 atggtatcga tggcgcgacc gtttcttgcg gatgcggaac tgctgtcaaa agcgcaatca
     4981 gggcgagccg atgagatcaa cacctgcatt ggctgcaacc aggcgtgtct cgatcagatc
     5041 ttcgttggca aggtcacctc gtgtctggtg aatcctcgcg cctgccatga aaccaaaatg
     5101 ccgatccttc ccgccatgca gaaaaaaaat ctggcggtgg tcggtgcggg acctgctggg
     5161 ctggcgtttg ccattaacgc ggcggcgcgt ggtcatcagg taacgttgtt tgacgcgcac
     5221 agtgagatcg gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga ggagttttac
     5281 gaaacgctgc gctattaccg ccggatgatc gaagtgacgg gcgtgacgct aaaactcaat
     5341 cacaccgtga cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc
     5401 gtgccgcgca ctccgcccat cgacgggatc gatcatccga aggtattgag ttatctcgat
     5461 gtactgcgcg acaaagcgcc ggtgggcaac aaagttgcca tcatcggttg tggcgggatt
     5521 ggttttgata cggcgatgta tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc
     5581 gggttctgta atgaatgggg gatcgacagc agcctacaac aggctggtgg cttaagcccg
     5641 cagggaatgc agatcccgcg tagcccacgg cagattgtga tgctccagcg caaagccagc
     5701 aaaccgggac aggggttagg caaaaccacc ggctggatcc atcgcaccac cctgctctcg
     5761 cggggtgtga aaatgatccc aggcgtaagt tatcagaaga ttgacgatga cgggctgcat
     5821 gtggtgatca acggcgaaac gcaggtatta gcagtggaca atgtggtgat ctgcgcaggg
     5881 caagagccaa accgcgcatt ggcgcagccg ctgattgata gcgggaaaac ggtgcattta
     5941 attggcggct gcgatgtggc tatggagctg gacgcacgac gggcaattgc ccagggaaca
     6001 cggctggcgc tggaga
//
LOCUS       DAZGXS010000203         6014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGXS010000203 DAZGXS010000000
VERSION     DAZGXS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            185..703
                     /locus_tag="ACHKG5_004502"
     CDS             185..703
                     /locus_tag="ACHKG5_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0870470.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            913..3054
                     /locus_tag="ACHKG5_004503"
     CDS             913..3054
                     /locus_tag="ACHKG5_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0870471.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3130..>6014
                     /locus_tag="ACHKG5_004504"
     CDS             3130..>6014
                     /locus_tag="ACHKG5_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0870472.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1378 a         1598 c         1936 g         1102 t
ORIGIN      
        1 gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct
       61 cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc
      121 ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt
      181 acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc
      241 aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat
      301 gctggtgcag gaattcctgc ataacgttac tgtcccgacc gatcctcagt ccggtcagcc
      361 tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca aagcggttcc
      421 gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac ttcactggtg
      481 gcgtacctct gtggaaggca aacaggagca ttatttcact acccgcctga ctgattcgac
      541 gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac gcgagttcac
      601 ccagttgctc gaagtttcac tggcctaccg caaaattgaa tgggaacacg tcaaatctgg
      661 cacctccggt gccgacgact ggcgcgcacc gctggaagca taagttaagc caacagcatc
      721 cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag
      781 gggttacgtt cacgggagat ggcggctgcc ggaaacttcg gcggtagcgt gcctgaacgc
      841 aagcgtagca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt ttccttaacc
      901 ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg cctgccaccg
      961 gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct tttttccctc
     1021 gatctctctc tggtcagcca gcagtttctc tcccttgaat tccagcagat cctcgacaaa
     1081 atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg
     1141 acctggtttg aactggggga gaacgacaaa aaccagaagc tgtacagcat gaaggtatgc
     1201 ccgccgctgt ggcgcacagg gctgcgccag aacttccgta tcttccagaa tgaggacatc
     1261 gcaagcatcc tcggcacgat attgcaggaa aacggggtga ccgagtggag cccgctgttc
     1321 agcgagccac atccttcccg tgagttttgt gtccagtacg gtgagactga ttacgatttc
     1381 ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg aggagcacgc gcaaaaaagt
     1441 accgaccaga gcctggtcct gtgcgatacc gtgcgttatc tgccggagtc ctttgagatc
     1501 ccctggaatc cgaacacccg taccgaggta agcaccctct gcatcagcca gtttcgctac
     1561 agcgcacaaa tccgcccttc ttccgtggtg accaaagact acacctttaa acgccccggc
     1621 tgggccggac gttttgacca ggaaggccag caccaggatt accagcgcac acagtatgaa
     1681 gtgtatgact atcccggacg tttcaagggc gcccacgggc agaactttgc ccgctggcag
     1741 atggacggct ggcgaaacaa cgcagaagtg gcgcgcggaa caagccgttc gccggagata
     1801 tggccgggac ggcgaattgt gctgacgggg catccgcagg cgaacctgaa ccgggaatgg
     1861 caggtggtgg caagtgaact gcacggtgag cagccgcagg cggtgccggg acgcaggggt
     1921 tcaggcacca cgctggataa ccattttgcg gtaataccgg cagacagaac atggcgacca
     1981 cagccgttgc tgaaaccgct ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca
     2041 ggcgaggaaa tcttctgtga tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc
     2101 tataacccgt caaaccagga cagttcatgc tggatccgtg tggcacaggc gtgggcaggc
     2161 accgggtttg gtaacctggc gataccgcgt gtgggtcagg aggtcattgt ggacttcctc
     2221 aacggcgatc cggaccagcc gatcattatg gggcgtacct accaccagga aaaccgcaca
     2281 cccggtagcc tgccggggac gaagacgcag atgaccattc gttcgaaaac ctataagggc
     2341 agcgggttta atgaactgaa gtttgacgat gcgacaggga aagaacaggt ctacatccac
     2401 gcgcagaaga acatgaacac cgaggtgctg aataaccgca ccacggatgt gataaacaac
     2461 catgccgaaa aaataggtaa caaccaggcg atcaccgtta ccaataacca gatcctgaac
     2521 attggcgtta atcagataca gacggttggt gtcaaccagg tggaaacggt aggcagtaac
     2581 cagattatca aagtgggatc aaaccaggtt gaaaaggtgg ggatcattcg tgcgctgacg
     2641 gtgggtgtgg cgtatcagac gacggtaggc ggcattatga atacctcggt ggcgttgttg
     2701 cagtcctcac aggtagggct gcataaatca ctgatggtgg gaatgggcta cagcgtcaat
     2761 gtagggaata acgtcacctt ctcggtgggc aagacgatga aggaaaacac cggacaaaca
     2821 gcagtttatt ctgccggtga gcatcttgaa ctctgctgtg gtaaggcaag gctggtgctg
     2881 acgaaggacg gaagcatatt tcttaatggt acgcacattc atctggaagg ggagtcggat
     2941 gtgaacggtg atgcgccggt gattaactgg aactgtggtg ccacacaacc tgtaccggat
     3001 gcgcctgtgc cgaaagattt accccccgga atgccggata tgcggcaatt ttgagtcagg
     3061 tgcaaaaaat caaaacggaa ctggtgcagt gaccagataa accatattaa aagcagaagg
     3121 aattgattca tgggaggaaa accggcggcg cggcagggtg acatgacccg caagggactg
     3181 gatattgtgc agggttcagc aggggtgctg ataggtgcgc cgacgggcgt ggcctgctcg
     3241 gtgtgtccgg gagggattac ctatgctaac ccggtgaacc cggtgctggg tgcgaaggtg
     3301 ctgccgggcg agacggacct tgcgctgccc ggcccgctgc cgtttattct ttcccgcgcc
     3361 tacagcagct accggaccag aacacccgcg ccggtggggg tgtttggtcc cggctggaaa
     3421 gcgcccttcg atatccgctt acagatacgc gatgaaggcc tgatactcaa cgacaacggc
     3481 ggcaggagca ttcactttga gccgctgttt cccggcgaga taagctacag ccgcagcgag
     3541 tcgttctggc tggcgcgggg cggggtggcg gagcagcaca gttcgcagcc gctaagcgcg
     3601 ctctggcagg tgctgccgga agatgttcgc ctgagtccgc atatgtacct ggcgacaaac
     3661 agccttcagg ggccgtggtg gatactcaac tggccggagc gggtgccggg ggcggacgag
     3721 gtgctgccgc cggagccgcc cgcataccgg gtgctgacgg gcgtggtgga tggcttcggg
     3781 cggacgctgg cctttcaccg ggcggcggag ggtgatgtgg cgggcgcggt gacgggggtg
     3841 acggacggcg cgggccgccg ttttcacctg gtgctgacca cacaggcaca gcgggcggaa
     3901 gtattccgta aacagcgcgc cacgtcttta tcttcccctg ccggtccccg ttctgcttcc
     3961 tcttctctgg ttttccccga cacgctgccc gccggtacag aatacggtgc cgataacggt
     4021 atccggctgg aggcggtatg gctgacacac gacccggcat acccggatga actgcccgcc
     4081 gcgccgctgg cgcgctacac gtacacggcc agcggagaac tgcgggcggt gtatgaccgc
     4141 agcgggacgc aggtgcgcgg gtttgcttat gatgcggagc acgccgggcg gatggtggcg
     4201 caccattatg cgggtaggcc ggagagccgc taccggtatg atgataccgg ccgggtgacg
     4261 gagctggtca acccggaggg gctggactac cgctttgagt acgggcagga ccgtgtgacc
     4321 atcacggaca gcctgaaccg gcgggaggtg ctgtacacgg aaggcgaggg tggcctgaaa
     4381 cgtgttgtga agaaggaaca tgcggacggg agcatcaccc gcagcgagta tgatgaggcg
     4441 gggaggctga aggcacagac ggatgcggcg ggacggcgga cggagtacag cctgcatatg
     4501 gcgtcgggtg cggtgacagc ggtgacgggg ccggacggca ggacggtgcg gtatggctat
     4561 aacagccagc ggcaggtgac gtcagtgacg tacccggacg ggctgcgcag cagccgggag
     4621 tatgatgaga agggaaggct ggcggcggag acctcgcgca gcggagagac gacgcggtac
     4681 agctatgatg acccggcgag tgagctgccg acagggatac aggacgcgac gggcagtaca
     4741 aaacagatgg catggagccg ttacggtcag ctgctgacct ttacggactg ctcggggtac
     4801 acgacgcggt atgagtatga ccggtacggt caacaaatcg ccgttcaccg ggaagaaggc
     4861 atcagcactt acagcagtta taacccgcgt ggccaactgg tcagtcagaa ggatgcgcag
     4921 gggcgtgaaa cccgttatga gtacagcgcc gcaggcgacc tcaccgctat cgttgccccg
     4981 gacggcagcc gcagtgagat acagtatgat gcgtggggaa aggccgtcag caccacgcag
     5041 ggcggtctga cgcgcagcat ggggtatgac gctgccgggc gcatcaccgt gctgaccaac
     5101 gagaacggca gccagtccac gttccggtat gacccggtgg acaggctgac tgaacagcgc
     5161 ggttttgacg gccggacgca acgttaccac tatgacctga ccggaaaact cacgcagagt
     5221 gaagacgagg ggcttatcac cctctggcac tacgatgcgt cggaccgcat cacgcaccgg
     5281 acggtgaacg gcgacccggc agagcagtgg cagtatgatg agcacgggtg gctaaccacc
     5341 ctcagccata ccagtgaagg ccaccgggtg tcggtccact acggctatga cgataaaggc
     5401 cgcctgacgg gtgaacggca gacggtggag aacccggaga cgggggagat gctgtgggag
     5461 catgagacgg ggcacgcgta cagcgaacag gggctggcga cccgtcagga gccggacggt
     5521 ctgccgccgg tagagtggct gacgtatggc agcggttatc ttgcggggat gaagctgggc
     5581 ggaacgccac tggtcgagta catgcgggac cggctgcacc gtgagacggc ccgcagcttc
     5641 ggcggggagg catatgaact tgccaccgcc tggaatacca gcggccagct ccggagcagg
     5701 cacctgaacc ttccgcagct tgaccgtgac tacgactgga acgacaacgg acagctaatc
     5761 cgcatcagcg gcccgcagga gagccgggag taccgttaca gtgacacggg aaggctgacg
     5821 ggcgtccaca ccacggcagc gaacctggat atcgatatcc cgtatgcaac ggacccggca
     5881 ggaaaccggc tgccggaccc ggaactgcat ccggacagca cgctcacggc gtggccggat
     5941 aaccgcatcg cggaagatgc gcactatatc tatcgctacg atgaatacgg caggctggcg
     6001 gagaagacgg accg
//
LOCUS       DAZGXS010000204         5939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGXS010000204 DAZGXS010000000
VERSION     DAZGXS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            136..582
                     /locus_tag="ACHKG5_004505"
     CDS             136..582
                     /locus_tag="ACHKG5_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP0870473.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            593..2032
                     /locus_tag="ACHKG5_004506"
     CDS             593..2032
                     /locus_tag="ACHKG5_004506"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP0870474.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2022..3092
                     /locus_tag="ACHKG5_004507"
     CDS             2022..3092
                     /locus_tag="ACHKG5_004507"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0870475.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3092..4840
                     /locus_tag="ACHKG5_004508"
     CDS             3092..4840
                     /locus_tag="ACHKG5_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP0870476.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4890..>5939)
                     /locus_tag="ACHKG5_004509"
     CDS             complement(4890..>5939)
                     /locus_tag="ACHKG5_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP0870477.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEA
                     KPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLK
                     DFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVAL
                     GNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQL
                     AELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKE
                     ADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRA
                     LREYNEMVDDYNSLR"
BASE COUNT         1388 a         1511 c         1497 g         1543 t
ORIGIN      
        1 aatatgtgcc ttacgccgca aaatttatcc ttcactctgc tttatattgt cacgacttca
       61 cgacatatcc gcgctcatca caaacccgcc ccgctattac aattaactga aatatttttt
      121 catggagtcc gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc
      181 aatggcgctt attccgttct gaagcagttg gcgacaatgg cgttacaaaa cggttttatc
      241 accgactcac accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctccaccggt
      301 tttggttctg gtgtcgccgt gccgcacggt aaaagcacct gcgttaaaca accgttcgtg
      361 ttattcgccc gcaaagcgca ggctattgac tggaaagcca gcgacggcga agacgtcaat
      421 tgctggatct gcctcggcgt accgcaaagt ggcgaagagg atcaggtcaa aatcatcggc
      481 acactgtgtc gcaaaattat tcaccaggaa tttattcatc aactgcaaca gggcgatacc
      541 gaccaggtgc ttgccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc
      601 atccttacgt attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt
      661 ggcggaagcc ctggaacaga aagcccgttc tctcggccat accattaaag tggaaactca
      721 agggtccagc ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt
      781 gattctcgcc accgggcgtg gcctgagcgg tgacgatcgc gcacggtttg ccgggaaaaa
      841 agtttatgag attgccatct cccaggcgct gaaaaatatc gatcagattt tcagtgaatt
      901 accgacaaat tcgcagcttt ttgccgcaga tagcggcgtg aagctaggca aacaggaagt
      961 acaaagcggc agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt
     1021 catcggcggt ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta
     1081 taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct
     1141 tggcttcacc tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa
     1201 accggctttc gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg
     1261 cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt
     1321 cgtcttctgg ttccgtaaag tccgtcttgg caaagcatta caaccgctgc tcggctccat
     1381 gcttatccca ttcgttactc tgctggtttt tggcgtgctc acttactacg ttatcgggcc
     1441 ggtgatgtcc gacctcatgg gcgggctact gcacttcctg aataccattc ctccatcaat
     1501 gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat
     1561 caataaaacc gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta
     1621 cgccatcgtc ggcgttgtcc ccgtcgcggc gggtcttgcg acgttcattg cgcccaaact
     1681 gtttacccga caggaaaaag aggctgccag tagtgccatt gtggtcggtg ccacagtcgc
     1741 aaccgaaccg gctattcctt acgctctggc agctccgttg ccgatgatta ccgccaatac
     1801 gctggcgggc ggcattaccg gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc
     1861 tggtttgggt atctttgacc cgctgatcgg cctgatgtcg ccggtaggtt cgttttatct
     1921 ggtgctggcg attggcctcg cgctgaacat ttcattcatt atcgtcctga aaggattgtg
     1981 gttacgccgc aaagcgaaag ccgcgcaaca ggagcttgtc catgaacatt gagttactgc
     2041 aacagttgtg cgaagccagc gccgtcagcg gcgatgaaca ggaagttcgc gacattctga
     2101 taaacacgct ggaaccttgc gttaatgaga tcacctttga tggtctgggc agctttgttg
     2161 cccgtaaggg aaataaaggt ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct
     2221 ttatggtcac ccacatcgac gagagcggtt ttctgcgctt taccaccatt ggcggctggt
     2281 ggaatcagtc gatgctcaac caccgggtaa caatacgcac acacaaggga gtgaaaatcc
     2341 ctggtgtgat tggttccgta gcgcctcatg cgttaacgga aaagcaaaag caacaaccgc
     2401 tgtcatttga tgagatgttc attgatattg gcgcgaacag tcgcgaagaa gcgaaaaaac
     2461 gcggtgcgga aattggcgat tttattagcc cggaagccaa ttttgcctgc tggggcgaag
     2521 ataaaatagt cggcaaggcg ctggataatc gcatcggctg cgcgatgatg gctgagctac
     2581 tacagacagt aaataaccca ggaattacgc tgtacggcgt cggcagcgtg gaagaagaag
     2641 ttgggctacg cggggcacaa acctcggctg aacacactaa accggatgtg gtgatcgtgc
     2701 tggataccgc cgtcgcgggc gatgttccgg gcattgataa cattaaatac ccgctgaaac
     2761 tgggcaacgg gccggggctg atgctgtttg acaagcgcta cttacccaac cagaaactgg
     2821 tggcggcgtt aaaaaactgt gccgcacata acggtttacc gctgcaattt tccaccatga
     2881 aaaccggagc gacggatggc gggcgctaca acgtaatggg cggagggcgt ccggttgtcg
     2941 cgctgtgtct gccaactcgt tatctgcacg ctaacagcgg tatgatttca aaagccgatt
     3001 atgatgctct gctcacgctg atacgggatt ttctgacgac cttaactgcg gagaaagtca
     3061 acgcgtttag ccagttccgt caggtggatt aatgctaaac gaacgccagt taaagattgt
     3121 cgatctgctg gagcaacagc cgcgcacgcc tggcgagctg gcgcaacaga ctggcgtttc
     3181 aggcaggacc atcctgcgtg atattgacta tctcaacttt acccttaacg gcaaagctcg
     3241 tatctctgcc agtggcagtg cgggctatca gttggaaatc ttcgagcgcc gcagcttttt
     3301 tcagttgctg caaaagcacg ataacgacga tcggctgctg gcgctgttat tactgaatac
     3361 ctttactccc cgtgcgcaac tcgcctcggc gcttaatttg ccagaaactt gggtcgcaga
     3421 gcgtctgccc cggttaaaac agcgttatga acgcacttgt tgcctggcca gtcgtcccgg
     3481 tttgggccat ttcattgatg aaacagaaga gaaacgcgtt atcttgctgg cgaacttgct
     3541 acgcaaagat ccgtttttaa tacctttggc tggcgtaaca cgagacaacc ttcagcattt
     3601 atccacggcc tgcgacaacc aacaccgctg gccgctcatg cagggggatt atctctccag
     3661 cctgattctg gcgatttacg ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc
     3721 cggtgacgag ataaaacaaa tcgttgaaca taacggtctg tttcttggtg ataacgctgt
     3781 aagaaccctg acaggtttga tagagaaaca gcatcagcaa gcgcaggtaa tttctgccga
     3841 tcatgtgctg gggttgctgc aaagggtgcc aggtctcgcg tcattgaata ttattgatac
     3901 gcagctggtt gagaacatta ccggtcattt attacgttgc cttgccgcac cagtgtggat
     3961 tgctgagcat cgccaaagca gcatgaataa cctgaaagcc gcctggcctg cggcctttga
     4021 tatgagtctg cactttatta cgctactgcg tgaacagctc gatattcccc ttttcgacag
     4081 cgatctgatc ggtttgtatt ttgcctgtgc gctggaacga catcaaaacg aacgccagcc
     4141 gatcattttg ctctcggacc agaacgcgat tgccactatt aatcagctcg ccattgagcg
     4201 cgatgtttta cattgtcggg tgattattgc ccgtagctta agcgaacttg ttgccattcg
     4261 cgaagagatt gagccgttat tgatcattaa caacagccat tatttactgg atgacgcggt
     4321 aaataattac atcaccgtaa aaaatatcat tacggctgcc ggtatcgaac aaataaaaca
     4381 ttttctggcg acggcattta ttcgccagca accggagcgt tttttctctg cccccggaag
     4441 ttttcattat tcgaatgtac gcggtgaaag ctggcaacat attacccggc aaatttgtgc
     4501 gcaattagtc gcacaacacc atattaccgc cgatgaagcg caacggatca tcgcccgcga
     4561 aggcgaaggt gaaaacctga ttgttaatcg cctcgccatc ccacattgct ggagcgaaca
     4621 ggagcgacgt tttcgtggat tttttattac cctcgcccaa ccagttgagg tgaataacga
     4681 agtcattaac catgtcttga tcgcctgcgc cgccgccgat gcgcgtcatg agctaaaaat
     4741 atttagctat ctggcaagcg tattgtgtca gcatccggca gaagttattg ccgggctaac
     4801 aggatatgag gcatttatgg agttacttca caaggggtga actgtgtcgg atgcgccgca
     4861 tccgacactg catttatcat caaaaacgtt taacgcaggc tattatagtc atcaaccatt
     4921 tcgttgtact cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct tgtgtttgca
     4981 tcctgaagat gctctttatc ccagtcagag tacgggactt tatctctgac gcggcgaaca
     5041 tattttttag tctcgagttg atactggtct gccgaattaa tgaacgagaa attcatgccg
     5101 cctttatccg cttctttggc ctgcgccacc agcgtttcca gttcagacac tttcttcatt
     5161 gccgcatcaa catcaaactt ctcttgcgat atcaggttat taatttgttt cgctgaaatc
     5221 atgactgcca gagaatagta gtggaatgtt ttcccttctc tttcttcaat atttttcagt
     5281 tccgcaagct ggcgcttatc attaatttcc tgaatcgccg catgataaga ttctgctaca
     5341 ggttcaaagg cttccagatt ttttaagaat gtctggtgca gcgttttccc tttcgcaaac
     5401 gcatcgtctt tataattttc ctgggtgtaa tatttatcca tttcgttaat ggtattaccc
     5461 aacgccacgg cagcatcgat ataactcacc gccacgccat caattggttg cagcgcaggc
     5521 gttaacgcca cagcactttt tacgcctttt tcacactcag cgaggttgga ttcactaatg
     5581 ccgtagatgc cataaacagt actctcttta ccggtaggcc cctgtttaaa atctttcagc
     5641 cagtcagcat aacgcgccag actgcgctgt accgggatct gtaacttgtt gtagcacttg
     5701 atatagacat ccattttttc gtcgaaaacc gcttgttcat cgaccagcgg ttgcgcaggt
     5761 gtttcaggtt tagcatctgc ttttgcaacg ggagcttcag taggtttcgc ttcaggagct
     5821 ggtgcggcag gttgactatt ggcaggcagg ggcgtttctg tttcggcttt tttatcatca
     5881 caacccgtca gaccgagggc catgatggcg actataatag cggaagataa taaattcct
//
LOCUS       DAZGXS010000205         5927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGXS010000205 DAZGXS010000000
VERSION     DAZGXS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..2409
                     /locus_tag="ACHKG5_004510"
     CDS             <1..2409
                     /locus_tag="ACHKG5_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0870478.1"
                     /translation="VAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKP
                     GIPRPALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDT
                     CENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKP
                     GAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAI
                     HREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRK
                     QPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLS
                     SPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDK
                     ALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPGSVKVTADEVNKIMQAANIS
                     QPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQKAEQNSPNT
                     QQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFDGENQPEVQ
                     ENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMI
                     DLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTMKWWLKQSR
                     EAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRSYERQGSPC
                     PWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPS
                     QADS"
     gene            2482..2733
                     /locus_tag="ACHKG5_004511"
     CDS             2482..2733
                     /locus_tag="ACHKG5_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0870479.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            2768..2955
                     /locus_tag="ACHKG5_004512"
                     /pseudo
     CDS             2768..2955
                     /locus_tag="ACHKG5_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(3430..3516)
                     /locus_tag="ACHKG5_004513"
                     /pseudo
     CDS             complement(3430..3516)
                     /locus_tag="ACHKG5_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3722..4048)
                     /gene="ynfA"
                     /locus_tag="ACHKG5_004514"
     CDS             complement(3722..4048)
                     /gene="ynfA"
                     /locus_tag="ACHKG5_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0870480.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            4183..4524
                     /gene="ynfB"
                     /locus_tag="ACHKG5_004515"
     CDS             4183..4524
                     /gene="ynfB"
                     /locus_tag="ACHKG5_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0870481.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4559..5119
                     /gene="speG"
                     /locus_tag="ACHKG5_004516"
     CDS             4559..5119
                     /gene="speG"
                     /locus_tag="ACHKG5_004516"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0870482.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5122..5832)
                     /gene="ynfC"
                     /locus_tag="ACHKG5_004517"
     CDS             complement(5122..5832)
                     /gene="ynfC"
                     /locus_tag="ACHKG5_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0870483.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
BASE COUNT         1779 a         1383 c         1376 g         1389 t
ORIGIN      
        1 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
       61 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc
      121 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      181 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atccgatccg
      241 atgaacgtca cttttgacaa cctggcccct gaagtacaga acgctgtcat ggttaagttc
      301 gacacatgtg aaaacatcac tgttgatatg gtgattagcg cacaggaatt gttgcaggaa
      361 gacatggcaa cattcgacgg ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      421 gctatgtatc cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct
      481 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      541 gaacgcaagg aagccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
      601 caacagcaca ctgaaaattc aacaggaaaa atcaacccgg tcattgctgc cattcatcgc
      661 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
      721 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
      781 gacaacggcc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
      841 cttcgctacg accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac
      901 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
      961 gaagatgaag gaacaaatca gagcgcaact gatactctct cgtcgccagt accagaaact
     1021 gatgcagtgg aaacggcaat tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa
     1081 ccatctgtag aacgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa
     1141 tacggtcgcg caaacaaact ttctggtctg gataaggcgc tgtctgctgg ggctactgaa
     1201 atcacgaaag aagaatattt cgcccgcaaa aacggtacat actcaggttc acaacaaaat
     1261 actggtgcat ctgacacgac cgcacaacca gggtcagtaa aagttaccgc tgacgaagta
     1321 aacaaaatta tgcaggcagc caatatcagc cagcctgacg ccgaggaact gcttgcagta
     1381 tcacgtggtg aatttgttga agggattagc gacccgaatg atccgaaatg ggttaagggg
     1441 atccagaccc gcgattctgt gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa
     1501 gcggaacaaa acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgaacca
     1561 gtagtgcaac aggaaccgga aaagatctgc accgcctgcg gtcagagcgg tggcggcaac
     1621 tgccctgatt gtggtgcggt gatgggtgac gcaacatacc aggaaatatt cgatggagag
     1681 aatcagcctg aagttcagga aaatgatccg gaggaaatgg aaggtactgc acatcagcac
     1741 aaggagaaca ctggcggcaa tcagcatcat gccagcgata gtgaaactgg cgaggcgtca
     1801 gatcccttaa ttaaggcgaa cggtcatcat aatctcacat ccaccagcag agcggggatt
     1861 catctgatga tcgatcttga aaccatggga aaaaatcccg atgccccgat tatctcaata
     1921 ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac cggaatttag taagactatc
     1981 gatctggata ctgctggcgg agtcattgat cgggacacca tgaaatggtg gcttaaacaa
     2041 tcacgcgaag cgcaatctgc cattatgacc gatgaaatcc cgttagatga tgcactgtta
     2101 caattgcggg aatttatcga cgaaaactcc ggtgaatttt ttgttcatgt ctggggaaat
     2161 ggagccaact tcgacaacac gattttgcgc cgttcatacg aacggcaggg gagcccctgc
     2221 ccgtggcgtt actacaacga tcgcgatgta cgcacaatcg ttgagctggg gaaagccata
     2281 gacttcgatg ccagaacggc tattccattc gaaggtgagc gccataatgc acttgatgac
     2341 gcccgttacc aggcaaaata cgtttcagct atctggcaaa aactgatccc gagtcaggct
     2401 gattcttaat gttcaactgt cgctggttgt gactggtatt ctgcaaccgg cgctcgtctg
     2461 atgtaagaga taaagaaatc gatgagcgaa gtaatcatga ttgtctctcc cggcaaatgg
     2521 gtatccgaag agcagttaat tgcgctgaaa ggaataaaaa aaggtacgtt aaaaaaggcc
     2581 cgggaaaaat cgtttatgga aggaagggaa tataagcatg tcgctcatga cggtatgcca
     2641 tgggataaca gtccatgctt ttacaacctg gaagaaattg atcgctggat tgagcgccag
     2701 gcatcagcaa gaccaagacg tcatcttact tgactaaaag ccacactaac taatgagaga
     2761 agttgaaatg aaatatccga caggcgtgga aaaccatgga gggaaattac gtatctggtt
     2821 tgtttataaa ggcgtaagag tcagggaaaa cctggggttc ctgacacagc aaaaaacagg
     2881 cgcgttgcag gtgagctacg cgcctctgtt tgttacgcaa taaaaactgg tgttttcgac
     2941 tatgcaaaac agtttccctc ctcacgcaat ctggaaaaat ttggtgaggc ccgacaagat
     3001 ttaaccataa aagaactggc tgaaaaattt ctggcactga aagaaactga agtcgcaaaa
     3061 acatcactca acacataccg tgccgtcatc aaaaatatcc tgagcataat cggtgaaaaa
     3121 aatcttgcct catcgattaa taaagaaaaa ttactggagg ttcgtaaaga gctactgact
     3181 ggataccaga tccccaaaag taactatatt gttacacaac cagggagatc ggctgtaact
     3241 gtaaataatt acatgacaaa tcttaacgcc gtgttccagt ttggtgttga taacggttac
     3301 ctggcagata acccgtttaa ggggatctcg ccattaaagg aatcaagaac cattccggat
     3361 cctctttcgc gggaggaatt catccgtctt atcgatgcgt gcagaaatca gcaagccaaa
     3421 aatttatggt gtaataccgt cctcagtggt gatttttaat gtgacgaaat tacgccccgg
     3481 acaggtaaca aaaacttcag cctttacgat cttcatgttc gattccttgc atcgcttgtc
     3541 gtgatgcatg aaatctacgc aactgagcta ctaccatata agtatgaaga tcgaaaaaag
     3601 ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga atggttgaca aaagatgaaa
     3661 tagaatacct tttgtcagct gacacttcct cgtatcttat tgataaaatg gatttatgtt
     3721 cctacgtgcg cccccagccc acaacaatga tcaacatgcc gcaaagcgca atcaacggcc
     3781 ccgtccagtc ataaagagtc agtttcacgc catccacaac gcgcagccac atcaacgccg
     3841 tgcagacata aacgccacca taagccgcgt aaacacgccc actcgccgca ggatgcaacg
     3901 ttaacaacca gacaaacagc gccagtgaaa tccccgccgg aagcaacagc cagatactgg
     3961 cgtttcgttt taaccacaac cagggcagaa agcatccaat aatttcacac agcgcagtag
     4021 caaaaaatag taacgttgtt ttaatcatct ttgtcactta ttgacatcat gtatagttat
     4081 agggcgacat aatatcatca atataaacac cctcctggta cgttttgcgt tcgcagtgga
     4141 tggtgtagaa tcatctttaa tcattcatac agggaatgaa ttatgaaaat cactctcagc
     4201 aaacgaatcg acctgctcgc ttttctgctg ccttgcgcac tggcattgag cacaactgtt
     4261 catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag ccgccagcac
     4321 gccgctatgg aaaaagagca atggaatgac acgggcaatc tgcgccagaa agtgaataaa
     4381 cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga taaatgcgag
     4441 caaagcgcca acatcaatgc ctactgggag ccaaatactt tgcgctgcct ggaccgtcga
     4501 actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag gacacgttat
     4561 gccaagcgcc cacagtgtta agctacgccc gctggagcgt gaagatttac gctatgtaca
     4621 tcaactcgac aataacgcca gtgtgatgcg ttactggttt gaggaaccct acgaagcctt
     4681 tgttgaactc tctgatctgt atgataagca tattcacgat cagagcgaac gacgctttgt
     4741 ggtggaatgt gacggcgaaa aagccggtct ggtggagttg gtggaaatta accatgtaca
     4801 tcgccgcgca gaatttcaga taattatctc cccggagtat caggggaaag gtctggcaac
     4861 ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt ctcaatctct ataagctgta
     4921 tctgatcgtt gataaagaga atgaaaaagc gattcacatt taccgcaagc ttggcttttc
     4981 ggttgaaggt gaattgatgc acgagttctt tattaatggt caatatcgta atgccattcg
     5041 catgtgtata ttccagcatc agtatctggc agagcacaaa acaccgggtc agactctcct
     5101 gaagccgacc gcacaatagc attaataata atcgatcgta tttttgatgg tgtaaacccg
     5161 ttcgacggcg ggttttactc cttcatcaac aatgatcagc tgacagccca ccggattggc
     5221 gtgactgtca tattcacagc tctgttttac attaccaact cgttgattat tcagtaaagt
     5281 aaccgctgtg taatctaatt ttttgatcgg gtccgttgat ggcgtggcgc tgacagataa
     5341 tgttttgtcg ttacttttcg tggttttacc cagtggataa ccctgatcat catagcgata
     5401 ttccatctgc atttgtttgc tgctggcttt aatcacgaag ccattatcat cggtttccca
     5461 actcaccccg gcagaaggta attctgctag ctggcatttt ccctgtaaac gtactctctt
     5521 ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt accagagcga ccacggtatt
     5581 attttccaga tccagtaatt cgagtgaatc aaaacagcct tcttccgaca aagtccccga
     5641 aacacgtttc gtcacttcac cttgctcatc cattaatgtc tgagtgaaat cttttaccgg
     5701 accacgcagc ggatcaaaat cgaattcatt agagaaactg gccatctcag gagtaaatga
     5761 aagtgttacc tctgtgcggt cacatcctgt gagtaatatc gcgagtaagc atggcagtaa
     5821 tttgtatttc acatcagtca ccagagagta gagatgattc tcaatcatag tagcaaatac
     5881 agtactttac acgtgaaatg ctatgcttaa agaagttatc ttcgcgt
//
LOCUS       DAZGXS010000206         5881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGXS010000206 DAZGXS010000000
VERSION     DAZGXS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            10..1053
                     /locus_tag="ACHKG5_004518"
     CDS             10..1053
                     /locus_tag="ACHKG5_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0870484.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            1046..1507
                     /locus_tag="ACHKG5_004519"
     CDS             1046..1507
                     /locus_tag="ACHKG5_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0870485.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            1541..2257
                     /locus_tag="ACHKG5_004520"
     CDS             1541..2257
                     /locus_tag="ACHKG5_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0870486.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2290..2571
                     /locus_tag="ACHKG5_004521"
     CDS             2290..2571
                     /locus_tag="ACHKG5_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870487.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2568..2795
                     /locus_tag="ACHKG5_004522"
     CDS             2568..2795
                     /locus_tag="ACHKG5_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870488.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2788..3099
                     /locus_tag="ACHKG5_004523"
     CDS             2788..3099
                     /locus_tag="ACHKG5_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870489.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            3227..3445
                     /locus_tag="ACHKG5_004524"
     CDS             3227..3445
                     /locus_tag="ACHKG5_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP0870490.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            3447..4004
                     /locus_tag="ACHKG5_004525"
     CDS             3447..4004
                     /locus_tag="ACHKG5_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0870491.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            4295..4450
                     /locus_tag="ACHKG5_004526"
     CDS             4295..4450
                     /locus_tag="ACHKG5_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0870492.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            4570..4914
                     /locus_tag="ACHKG5_004527"
     CDS             4570..4914
                     /locus_tag="ACHKG5_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP0870493.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            5036..5308
                     /locus_tag="ACHKG5_004528"
     CDS             5036..5308
                     /locus_tag="ACHKG5_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870494.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            5310..>5881
                     /locus_tag="ACHKG5_004529"
     CDS             5310..>5881
                     /locus_tag="ACHKG5_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0870495.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
BASE COUNT         1557 a         1301 c         1718 g         1305 t
ORIGIN      
        1 gaggtgagta tggctaatgc ctggctcaga ttgtggcatg acatgccaaa tgaccccaag
       61 tggcgaacga ttgccagggt atcaggacag ccaatcgcaa cagtgatggc agtgtatatc
      121 catcttctgg tgagcgcgtc acgaaatgtc acgacatgtc acggcgtgtc actacgtggt
      181 cacattgatg tcacgacgga agatttagca agtgcgcttg atgtgacgga agacgtaatt
      241 gattcaattt tgcatgcaat gcaggggcgg gttctggatg gtgaccttat ttccggatgg
      301 gaaaaacgtc aggtgctgaa agaggacaat ggtaacgttt cgcaaacggc aaaatccccg
      361 gcagagcgca agagagcgca gcgggagcgg gaaaagctgc ggaaacataa tgctgattgt
      421 cacgatgagt cacgacgtgt cacgcatctg tcacgacaag tcacgacaga taaagataca
      481 gataaagata cagatacaga attaaacccc acacataacg cgcgcgagag tattccgacc
      541 agtgagtcga atggtgcgcc gttgcagaca gccgaacctg aatacctgga cggcctgagc
      601 gaaccgatcg ggaaattttc gatgactact gtctggcagc cgtcgccgga ttttcgacaa
      661 cgggcagcag tgtggggtat ggctctgcct gagccggaat ttacacctgc tgagcttgcc
      721 gcattccggg attactggat ggcggagggg aaggttttca cgcaggttca gtgggagcag
      781 aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa aaccagtcag caagggggga
      841 agccatgcag catcaggtgg cacggcatca cgggcagttc aggaaatccg ggctgcacgc
      901 gaacagtggg aacgtgacaa cggatttatc agcaacggaa acggcctgga agctgtggga
      961 gctcatgggg gaggtgtatt cgaaccgctg gactcagaag aacggggccg caccttcgaa
     1021 gctctggatt gcccagattg gtgcgatgac tgaacagcaa atccgtctgg tgtgccgtca
     1081 gtgcatggac cgctgccggg cgggtgaaac gtggcccccg gacctggctg agtttgttgc
     1141 gctgatttcg gagagtgggg caaatccatt tggtcttacg gtggatgccg tgatggaaga
     1201 gtaccggcgc tggcgcaatg aatcctggcg atacgacggg agtgataaat acccgtggcc
     1261 acagcctgtg ctgtaccaca tctgcctcga aatgcgtacc agagggattg agcgccagat
     1321 gacgcaaggt gagttaaaac gacttgcgga acggcaactg acgaaatggg caaagcatgt
     1381 tggtaacggg atgagtgttc cgccagtgcg acgacaactg gaaggggcga aacacccgca
     1441 agggccaacg ccaattgaac ggctgaaaca ggaatacgaa cgccggaagg cagctggttt
     1501 tatttgaatc tgagaaacga ttttgtcgga ggaaatatta atggaaaccg tatttgacgc
     1561 actgaaagca ctgaaaaaag cctcttcaca ggtagtggca tcgcgccttg gaatcagccg
     1621 cgaagatgct gtcaacgaac tgtggaaact gaagcgccgt ggtgaagcgg ataacaaggg
     1681 gtcgatgtgg tggctgactc agactggtga aagtgaacca gtgtcaccgg taccgaaagt
     1741 gacagcgcaa atgctgactg aggcgattga acatcatggc ccacaaacgg cggatgagct
     1801 ggcactgatg ttcgggatta cctcccgccg ggcgaattca tcactggcca tggcaatcag
     1861 caaagggcgt ctgattcgcg tgaatcaggg cggtaaattt cggtactgca taccgggcgc
     1921 tgatttaccg gcagagccga aagccgcatc cataacggaa acggatggta aagcctttcc
     1981 tcagccagca ggtgttgcgt taccagtcgg ggaagcggaa acacaggaag aaataaaaac
     2041 ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc accagaaagc atccggatgg
     2101 tctgatttta ccatcgctgc atgtggctaa ccgcgagctg cgccgggcaa aaggtcaggt
     2161 tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagtgcc gggatattct
     2221 ccgggatatt accgccacca gagaacagca gcggtgagtg ggtggaagac gtggtgccgg
     2281 gcggaaatca tgatactccg gcagtgtgcg ggaacgatga aggtaaaaag cgttggcgca
     2341 cttatcggac gaactgaagc ggcagtgaga acgaaggcac gggagctggg catcagcatg
     2401 atgttacgtg gtgattttca cccgtcggca aaatattctc agcgtgatat tgagctggcg
     2461 cggcaactgc atcagagagg catgcaaaga agggaaattg ccagaaaatt aggcatgccg
     2521 ctgcgcatag tgaataacta cgtttatttc gacaggaggg tgtctgcgtg aaaatcctgt
     2581 atcaggatta cggcccggtg gggcaggtgg ttatcagcag tactgtaatg gagtttcgga
     2641 agcataaccg tgtggtggat gctgtgctgt taacctgtcc ggggatatcg gcgagtcgtg
     2701 caggtgtgtt tattatgaag acgaaattat atggcagtaa ggcgtggata aagaaggcgt
     2761 atcgtgtagc gttgcaggag gttaacagtg agtgaaatta aagaaatgcc ggtagttcgt
     2821 gacggatatg gctactggac acatcctgaa tatgaaaaat tctgtgatgg tcgggaatat
     2881 atttcaacgg aagagtttaa cgcctggatg gaggaaaata atcttcaata cgtcctctgc
     2941 ttcagagatg aaggatgtgc tgaccttgat gcgtgtgatg ctgatatttc tgcatgggaa
     3001 ccggaacgac cagagggcga tggctggttt attggttcca ttcatgatac ggaagatggc
     3061 ccggtttgtg tctggttgcg aaataaggct gaagcataaa ggcgataaac caactaacaa
     3121 ctaaatactg aagatttaaa tcagaaacga tttttattaa atccttaacc ggagggattc
     3181 ctgcaccctc agaacatcag gaggccgccc gaaagggcgg taagaaatga aacattattt
     3241 agaaaaaaat tacccacgaa agagcagaac aacagagttt ctgtttttca ttctgtttat
     3301 agtgttgatg ataccgatat ccccgctatt actggtctgg ataattggaa ggacatttga
     3361 accagttatt gagctatata ccgatgtgac atgggaatca ttcagcgcac tgcacaataa
     3421 aattaatccg tataaggaaa actgatatga gcactattac cagagaacgc gcggagatta
     3481 aatcatacat cacaggcttc ctgagcgact cggcgcacga taacaagtct tcagacagcc
     3541 tgctggctaa tgtgtttcgt atcgcgctgg catcactgga agcagagccg atagcaatgg
     3601 tagtgcctga tgaaatggat ttgcttacct gccatctcga cggtgtaact aaaacatatg
     3661 ctgatggctg gaacgcctgc cgcgtcgcca tgcttcaggc cggaaacttt cgggaaaata
     3721 agaattcgtc aaccaacaat tttcgggaaa tctcggaaac gtcaaccaga tctccgataa
     3781 ctctggatgg ctggataagc tgtactgagc gaatgcctga aaagagccag aacgtgctta
     3841 tttcgatgaa tatcgatagc gaggctgggc cattaatata ttccgcacgc tatctcggag
     3901 gcacgttccg gcgcggaggt atagcagtta gtccgggtaa tgatcttagg caagcaaccc
     3961 actggatgtc gctaccagaa ccgccgcagg aggtgaatca atgacctggc ctgaagcatt
     4021 cacaacggta ggaattgcga tggcggtggc gctggtggtg tattcgattt gccgctgggg
     4081 ataaaaacgg tttgcgggaa aaggatagtt aagtagaatt gctgcgggtg cttgaggcta
     4141 tctgcctcgg gcatgaacac caacggcaga tagagaaaag ccccagttaa cattacgcgt
     4201 cctgcaagac gcttaacatt aatctgaggc tcaatccatg ctgaacacat gtaggttagc
     4261 ctcttacgtg ccgaaaggca aggagaagca ggctatgaag cagcaaaagg cgatgttaat
     4321 cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga ggaaagacct
     4381 ctgcgaggtg cgaatccgaa ccggtcagac ggaggtcgct gtcttcgtag actacgaatc
     4441 tgagaagtaa gagaccaggc gggggagtaa tctcccgcca cctctgatgt gtcaggcatc
     4501 ctcaacgcac ccgcgcttta ccatactgaa aatgctgttt gaatgttcat ctctgaaaga
     4561 ggactatgaa tgaaaaaggt attgattgca gcacttattt ccggtgtgtc ttttggcgct
     4621 tttgcacagc agggtggttt ccaggggcca gaagcagagc gttcaacagt agcgcaggca
     4681 aaagaactga aggatgatgc atgggttatc cttgaaggga gcatcgttaa aaaagtgggt
     4741 gatgaacgtt atgagtttcg tgacaatagc gggacaattg tcacggatat tgatgacagc
     4801 gtatgggccg ggcagaatgt ttctccgaaa gacaaagtaa gaattgaggg tgaaattgat
     4861 aaagacctga gcagtgttga agttgatgta aaggcactga aattattaaa gtaaccgccc
     4921 ctgcttgtta agcccgtctt actgacgggt tttctgtttg tacattccgg cgtattgcct
     4981 tacaattcgc gcagtcagcc tgaacaactg acacctgctg tcaccggaga atccgatgac
     5041 acaacacata aaatcccaca attctgaagc cgacccggaa attaagcagg ggaggcgttt
     5101 tcgtgcgcct cagtatggct ggtttcacta tctgttctgt acgatcgatg aggcagatat
     5161 gcttcaagag gcgtatctgc gtcgcggtgt ccgtgtggag cggagtctga acgctgatcg
     5221 tctgacctgg accgtttctg tatatcttcc tgttcgtgca catctgccac ggacacatgc
     5281 ctgctaccgt cagcgcgtct ggaggtaatg tgcgggtatt acttcgacct gttctggttc
     5341 cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc attgccagtt tttcatcgcg
     5401 gcagggtgct ggtggagccg gaaccgaaaa acatgcgggc gctgccatct ggagcggttc
     5461 ctgctgttcg ccagccgctg gcggaagata aatcactgct gccatttttc agcgatgagc
     5521 gggtgattcg tgcagctggc ggcgctggtg cactgtctga ctggttatta cgtcacgtga
     5581 aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgttatt cacagttacg
     5641 gtgctggcgc aatggtgttg tgctggcact gcgacaacca gctgcgcgac cagacctccg
     5701 aatcacttga gcaacttact caacaaaatc tgacagcctg gatgattgac gtcatacgcc
     5761 atgtaatgaa tggcacgcag gagcgggaat tatcgctggc tgaattatcc tggtgggcag
     5821 tctgcaatca ggtggtggac gcattacctg aggcagtatc gcgtcgctct ctgggattac
     5881 c
//
LOCUS       DAZGXS010000207         5764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGXS010000207 DAZGXS010000000
VERSION     DAZGXS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            71..613
                     /gene="fimA"
                     /locus_tag="ACHKG5_004530"
     CDS             71..613
                     /gene="fimA"
                     /locus_tag="ACHKG5_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0870496.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            833..1525
                     /gene="fimC"
                     /locus_tag="ACHKG5_004531"
     CDS             833..1525
                     /gene="fimC"
                     /locus_tag="ACHKG5_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0870497.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1556..4165
                     /locus_tag="ACHKG5_004532"
     CDS             1556..4165
                     /locus_tag="ACHKG5_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0870498.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4178..5185
                     /gene="fimH"
                     /locus_tag="ACHKG5_004533"
     CDS             4178..5185
                     /gene="fimH"
                     /locus_tag="ACHKG5_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0870499.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5196..5711
                     /gene="sfmF"
                     /locus_tag="ACHKG5_004534"
     CDS             5196..5711
                     /gene="sfmF"
                     /locus_tag="ACHKG5_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP0870500.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
BASE COUNT         1515 a         1233 c         1389 g         1627 t
ORIGIN      
        1 tcagtgtctt aaataaagta atcggttata tacggatgtg gagtcgataa atgagattga
       61 aggaatatat atgaaattaa gatttatttc gtctgcgctg gctgccgcac tattcgccgc
      121 tacgggtagt tatgctgccg ttgtagatgg cggtacaatt cactttgaag gtgaactggt
      181 gaatgctgcc tgttcagtga atactgactc ggcagaccag gttgtcacac tcggtcaata
      241 tcgtaccgat attttcaatg ctgttggtaa tacctctgca ttaattccat tcaccattca
      301 gttgaacgac tgcgatcctg ttgttgccgc taatgctgcc gttgcatttt ctggtcaggc
      361 tgatgcaatc aatgataatt tattggccat tgcatccagt accaatacaa caacagcaac
      421 gggtgtcggt attgaaatac ttgataatac atccgcaatt ctcaaacctg atgggaatag
      481 cttctcaacc aaccagaact tgatccccgg gaccaacgtt cttcattttt ctgcacgtta
      541 taaaggcacc ggtacaagtg catcagcagg gcaagcaaat gctgacgcga cttttattat
      601 gagatacgaa taatcaaaac cacgttgttt tgaattatat atcacgtctt ataacaaagt
      661 aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa tcgaaatcat gttcactttg
      721 tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt gtaagaaacc aaagcaatca
      781 tttctttata tttcttattt ttgccgtcag gaatacacaa ggcgtattaa ctatgatgac
      841 taaaataaag ttattgatgc tcattatatt ttatttaatc atttcggcca gcgtccatgc
      901 tgccggaggg atcgcattag gtgccacgcg tattatttat cccgctgatg ctaaacagac
      961 tgcggtatgg attagaaata gccataccaa tgagcgtttt ctggtcaatt cgtggattga
     1021 aaacagcagc ggtgtaaaag aaaagtcatt catcattaca ccgccactat ttgttagtga
     1081 acccaaaagc gaaaatactt tgcgtattat ttacaccggt ccaccgctgg cagcagatcg
     1141 tgagtctctg ttctggatga atgttaagac gattccttcg gtagataaaa atgcattgaa
     1201 cggcaggaat gttttgcaac tggcgatttt atcgcgcatg aagttatttc tccgtccaat
     1261 tcaactacaa gaattacccg cagaagcgcc ggatacactc aagttttcgc gatccggtaa
     1321 ctatatcaat gttcataatc catcaccttt ttatgtcacc ctggttaact tacaagtggg
     1381 cagccaaaag ttggggaatg ctatggctgc acccagagtt aattcacaaa ttcccttacc
     1441 ctcaggagtg cagggaaagc tgaaatttca gaccgttaat gattatggtt cagtaacccc
     1501 ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca atatcagagc taattatgaa
     1561 aatacccact actacggata ttccgcagag gtatacctgg tgtctggccg gaatttgtta
     1621 ttcatctctt gccattttac cctccttttt aagctatgcg gaaagttatt tcaacccggc
     1681 atttttatta gagaatggca cattcgttgc tgatttatcg cgctttgaga gaggtaatca
     1741 tcaacctgcg ggcgtgtatc gggtggatct ctggcgtaat gatgagttca ttggttcaca
     1801 ggatatcgta tttgaatcga caacagaaaa tacaggtgat aaatcaggtg ggttaatgcc
     1861 ctgttttaac caggtactcc ttgaacgaat tggccttaat agcagtgcat ttcccgagtt
     1921 agcccagcag caaaacaata aatgcatcaa tttactgaaa gctgtacctg atgccacaat
     1981 taactttgat tttgcagcga tgcgcctgaa catcactatt cctcagatag cgttgttgag
     2041 tagcgctcac ggttacattc cgcctgaaga gtgggatgaa ggtattcctg ctttactcct
     2101 gaattataat ttcaccggta acagaggtaa tggtaacgat agctattttt ttagtgagct
     2161 cagcgggatt aatattggcc cgtggcgttt acgcaacaat ggttcctgga actattttcg
     2221 cggaaatgga tatcattcag aacagtggaa taatattggc acctgggtac agcgcgccat
     2281 tattccgctg aaaagtgaac tggtaatggg agacggcaat acaggaagtg atattttcga
     2341 tggtgttgga tttcgtggtg tacggcttta ttcttccgat aatatgtatc ctgatagcca
     2401 gcaagggttt gccccaacgg tacgtgggat tgcccgtacg gcggcccagc taacgattcg
     2461 gcaaaatggt tttattatct atcaaagcta tgtttccccc ggcgcttttg aaattacaga
     2521 tttgcacccg acatcttcaa atggcgatct ggacgtcacc atcgacgaac gcgatggcaa
     2581 tcagcagaat tacactattc cgtattcaac agtgccaatt ttacaacgcg aagggcgttt
     2641 caaatttgac ctgacggcgg gcgattttcg tagcggtaat agtcagcaat catcgccttt
     2701 cttttttcag ggcacggcac tcggcggttt accacaggaa tttactgcct acggcgggac
     2761 gcaattatct gccaattaca ccgccttttt attagggctg gggcgcaacc tcgggaactg
     2821 gggcgcagtg tcgctggatg tgacccatgc gcgcagtcag ttagccgacg acagtcgtca
     2881 tgagggggat tccattcgct tcctctatgc gaaatcgatg aacaccttcg gcaccaattt
     2941 tcagttaatg ggttaccgct attcgacaca aggtttttat acccttgatg atgttgcgta
     3001 tcgtcgaatg gaggggtacg aatatgatta cgattatgac ggtgagcatc gggatgaacc
     3061 gataatcgtg aattaccaca atttacgctt tagccgtaaa gaccgtttgc agttaaatat
     3121 ttcacaatca cttaatgact ttggctcgct ttatatctct ggtacccatc aaaaatactg
     3181 gaatacttcg gattcagata cctggtatca ggtggggtat accagcagct gggttggcat
     3241 cagttattca ctctcatttt cgtggaatga atctgtaggg atccccgata acgaacgtat
     3301 tgtcggactt aatgtttcag tgcctttcaa tgttctgacc aaacgtcgct acacccggga
     3361 aaatgcgctc gaccgcgctt atgcctcctt taacgccaac cgtaacagca acgggcaaaa
     3421 tagctggctg gcaggtgtag gtgggacctt actggaaggc cacaacctga gttatcacgt
     3481 aagccagggt gatacctcga ataatgggta tacgggcagc gccacggcaa actggcaggc
     3541 cgcttacggt acgctggggg tcgggtataa ctacgaccgc gatcaacatg acgttaactg
     3601 gcagctgtct ggcggtgtgg tcggacatga aaatggcata acgctgagcc agcctttagg
     3661 ggataccaat gttttgatta aagcgcctgg cgcaggcggt gtacgcattg aaaatcaaac
     3721 tggcatttta accgactggc gcggctatgc ggtgatgccg tatgccacgg tttatcggta
     3781 taaccgtatc gcgcttgata ccaatacgat ggggaattcc atcgatgttg aaaaaaatat
     3841 tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc aattttgata cccgcatagg
     3901 cgtgcgggcg ctcattaccg ttacccaggg cggaaaaccg gtgccgtttg gatcactggt
     3961 acgggaaaac agtaccggaa taaccagtat ggtgggtgat gacgggcaag tttatttaag
     4021 tggtgcgcca ttgtctggtg aattactggt tcagtgggga gacggcgcga actcacgctg
     4081 cattgcgcac tatgtattgc cgaagcaaag cttacagcaa gccgtcactg ttatttcggc
     4141 agtttgcaca catcctggct cataaaggaa attatcaatg aagataatct gcagattatt
     4201 attggcgatg gcatgtttgt gtctggcaaa catatcctgg gctactgttt gtgcaaatag
     4261 tactggcgta gcagaagatg aacactatga tctctcaaat gtctttaata gcaccaataa
     4321 ccagccaggg cagattgttg ttttaccgga aaaatccggc tgggtaggtg tctcagcaat
     4381 ttgtccaccc ggcacgctgg tgaattatac ataccgtagt tatgtcacta actttattgt
     4441 tcaggaaact atcgataatt ataaatatat gcaattaaat gattatctat taggtgcgat
     4501 gagtctggtt gatagtgtga tggatattca atttcccccg caaaattata ttcgtatggg
     4561 aacagatcct aacgtttcgc aaaaccttcc gttcggggtg atggattctc gtttgatatt
     4621 tcgtttaaag gttattcggc cctttattaa catggtggag atccccagac aggtgatgtt
     4681 taccgtgtat gtgacatcaa cgccttacga tccgttggtt acacctgttt ataccattag
     4741 ttttggtggc cgggttgaag taccgcaaaa ctgcgaatta aatgccgggc agattgttga
     4801 atttgatttt ggtgatatcg gcgcatcgtt atttagtgcg gcagggccgg gtaatcggcc
     4861 tgctggtgtc atgccgcaaa ccaagagcat tgcggtcaaa tgtacgaatg ttgctgcgca
     4921 ggcttattta acaatgcgtc tggaagccag tgccgtttct ggtcaggcga tggtgtcgga
     4981 caatcaggat ttaggtttta ttgtcgccga tcagaacgat acgccgatca cgcctaacga
     5041 tctcaatagc gttattcctt tccgtctgga tgcagctgcg gcagccaatg tcacacttcg
     5101 cgcctggcct atcagtatta ccggtcaaaa accgaccgaa gggccgttta gcgcgctggg
     5161 gtatttacgc gtcgattatc aatgaggtac ggagaatgag aagagtattc tttagctgtt
     5221 tctgcgggtt actgtggagt tccagtggat gggcagctga ccctttagga acgattaata
     5281 tcaatttgca cggtaacgtt gttgatttct cctgtaccgt aaacacagcg gatattgata
     5341 agacggtaga tttaggcaga tggcctacga cacaattact gaacgctggc gataccacgg
     5401 cactcctccc ttttagcctg cggctggagg gatgtcctcc gggttcagtt gcgattttat
     5461 ttacgggaac gccggcatct gatgccaacc tgctggctct ggatgatccc gcaatggcac
     5521 aaaccgtcgc catcgaatta cgtaatagcg atcgctcccg gctcgcactg ggggaggcga
     5581 gcccgactga ggaagtagat gcaaatggca atgtcacact aaactttttt gccaattatc
     5641 gagcgttagc cagcggtgtt cggccaggtg tggcgaaagc ggatgcgata tttatgatca
     5701 attataatta atattatatt aattcgtata atttggcgta gtcgataagc tctacaattg
     5761 aatg
//
LOCUS       DAZGXS010000208         5744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGXS010000208 DAZGXS010000000
VERSION     DAZGXS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            75..1259
                     /locus_tag="ACHKG5_004535"
     CDS             75..1259
                     /locus_tag="ACHKG5_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0870501.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            1259..1771
                     /locus_tag="ACHKG5_004536"
     CDS             1259..1771
                     /locus_tag="ACHKG5_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0870502.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            1826..2191
                     /locus_tag="ACHKG5_004537"
     CDS             1826..2191
                     /locus_tag="ACHKG5_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870503.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2200..2355
                     /locus_tag="ACHKG5_004538"
     CDS             2200..2355
                     /locus_tag="ACHKG5_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0870504.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            2342..5145
                     /locus_tag="ACHKG5_004539"
                     /pseudo
     CDS             2342..5145
                     /locus_tag="ACHKG5_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5158..5646
                     /locus_tag="ACHKG5_004540"
     CDS             5158..5646
                     /locus_tag="ACHKG5_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870505.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
BASE COUNT         1364 a         1383 c         1697 g         1300 t
ORIGIN      
        1 ctcttacggt gattacgcgc gtggtgggtg atgattgttg ccgaatcatt taacggatac
       61 aaggggctga agctatgagt gaaactcgtt ttcatggtgc ccgtgttacg gaaagtaccg
      121 acctggtaac agcgattaat gatgttgatt ccagtgttat cggtatcgtg gcaacggcag
      181 atgatgcgga cgcggagctg ttcccgctga acaagcccac actgctgacc cgcgtcaatg
      241 acgtgctggg aaaatgcggg actacgggga cgctttatcg tgcgcttaag gctatcgcag
      301 accaggtgag cacaaaagtg atcgtcgttc gcgtggctga acacaaagaa gaaggcggta
      361 agacgcagga tcaactggtt atcggtggtt ctgaatctga cggcagctat acggggatgt
      421 atgcgctgct tgttgcagag caggatgaaa gcatcggata ccgtccgcgt attctggccg
      481 cgccggagct ggacacggag gcggtaacaa aatccctgtg cgtgattgcg ggtaaactgc
      541 gcgcgtttgt gtatgccaca tgtcatggtt gtaacacgat ggctgaggcg attacctacc
      601 gccagaaatt caacgaacgt gaggtgatgc tcttatggcc tgacttcatc gcctacaacc
      661 tgaaaagtgg caaaaacgaa acgttccccg cgcctgctta tgcgtgcggc cttcgtgcgt
      721 acattgacca tgagcagggc tggcacaaat cgctgtccaa cgttccggtt aaaaatgtgc
      781 ttgggatgtc gaggcatgtg ttctggtcgt tgcaggccga agacagtgat gccaacagcc
      841 tcaacaacaa agaaatcacg accattattc gtcgcaacgg gttccgcttc tggggcaacc
      901 gcacaccgga aacgaacgcc tacatctttg aggtgtatac ccgaaccgca caggtgctgg
      961 ctgattcaat tgcggaagcg cagtttgaaa ccatcgacag tccactgaca cctgcgaacg
     1021 tgaaggatgt tatcagtgcc atcagggcaa aactggattc actggttact gccgggaaac
     1081 tgattggcgc ggagtgctgg tatgacatcg aggataacag caccacgaat ttacgtcagg
     1141 ggcgtgtgcg tattcgctac aaatatacgc ccgttcctcc gctggaagac atggagcttt
     1201 accagtcgtt tactgatgaa ttctttggtc ccgcatttgc ggtgctggga ggtgcctgat
     1261 ggctgtacca aaacatcttc gcttttttac gctgtttgtg gatggtgaaa acgaagtggg
     1321 taaggtgacg tccgtcaccc tgcctaaact gacgcgcaaa accgacagct accggggtgg
     1381 tggcatgatg ggtgcggtaa gtattgacct cggcctggac gactccgcgc ttgatgcgag
     1441 ctttgtcatg gggggcgcag ttcgtgagct gttcctgaag tatggcggca cgattgacgg
     1501 cacgctactg cgttttgcgg gggaatacta caccgatgat gaaagcgacc tgtatgaagt
     1561 cgaaatgcgc ggacgtgtga cggaaattga tatgggggaa gccaaacagg gagaagccac
     1621 atcacacact tacgccatta aaaacaccta ctacaagctg agtgttaacg atcgcccgtt
     1681 gtgggagatt gacctgctga acttcattta ccggaaggac ggcaaggaca ttgtgcccga
     1741 tcgcatccgt tccgcgcttg ggcttggctg ataagtaata tgcaggcggc gcagtgcgtc
     1801 gcctctgact gaaaggagtt tcctgatgaa agagacgaaa aacatcgata ccgaaaacac
     1861 ggtagttact gacactgtga aagaaaccag tgagcgtggt gtaaaactta cccaaccaat
     1921 tgagcgaggc ggcgaaaaaa tcacgtatgt ggagatcacc ggggctattg agcaggctgg
     1981 atctctgcga gatttgtcgc tgtctgatgt gctgaatctg aaagcggaat ccatgtttac
     2041 gctgctgtca cgcgtgacat caccgcgact ggatgaagtg acgatcaaaa aaatggcatc
     2101 ccgtgacttt attcagttat gtgtggttgc cgtaaatttt ttgagcggtg cggactctgg
     2161 cgggaagaac gaacaggcga cggaagcctg atcacggttg tgtgctttga gcacatagaa
     2221 gactttgtgg cagatattgc cgttattttt aactggtcgc ccgccgaaat cttcatgatg
     2281 acgcccggcg aagtggttag ctggcgtgag cgggcggcac ttcgcagcgg gaatgcagac
     2341 aatgaagact cttgatatcc gggtcgcttt cagcgccgtt gacaggctga cccgacctgc
     2401 cgaaaacgca cgccgcctga tggggcagtt tggtgactcc atccagcgaa cgcagggggc
     2461 gatcaaaaat ctcgagcgtc aggcgcgatc atttgagcgc gcccgcgacg ctgtcagtaa
     2521 agcggatgct ggcatcgtga aagcacgacg ccagcttaac gcccttaatc agttacaacg
     2581 cacgggtaca gtgctcagcg aaaaacaaca aaagctgatg cagcagttaa gcacccggct
     2641 tgaacgcctg aatgaatcgc gcacacggga aattcagaaa atgagggaac ttggcggaga
     2701 gctgaaacgc cacggcattt ccctgacagg cagcgataac accattcagc aggccatcag
     2761 acgcaccgaa caatacaaca accagcttga acgcgaacgg caggcgcttg cgcgtgtaac
     2821 gcaggcgcgt gagcggtatt cgcacgcgca ggaaaccgcg ggaaaactga aaacaggtgg
     2881 tgcgctggca attggtgcgg cagcggcggg cggctatgct gccgggcgtt ttttgcagcc
     2941 tgcgatcggg ttcggcaaag agatgtcccg cgttcaggca ctgacgcgaa tcgacaaaaa
     3001 cagcccgcag tttaaggcgc tgcgtgagca ggcgttaaaa cttgctgaaa cacagtttac
     3061 tgcgagtgat gccgccagtg ggcagagctt tctggcaatg gctggtttta ctccgcaggc
     3121 cattcaggcc gcattgcccg gtgttcttaa tatggcgctg gcaggtggcg tcgaactcgg
     3181 cgagacggct gatataggct ccaatatcct cacacagttc aacctgacag ccgatcaaat
     3241 ggaccgggtg ggcgatacgc tgacagcagc atttacccgg accaatactg atttacgcgc
     3301 gctgggcgaa accatgaagt ataccggtcc ggttgccgca aaacttggta tcagtcttga
     3361 agaagcggcg gccatggccg ggatgcttgc caataatggt cttcgcggaa gcgatgctgg
     3421 cacggccatg cgcgcaagtc tgtcccgcct tgcatcaccg ccaaaagctg cggctgatgc
     3481 actgaaagag ctgggggtgt cagttgctga cgccagaggc aaaatgcgcc cgatggagga
     3541 tgtgctgctt gatctctata aggcgacaca aaaatacgga caggtggacc aggtctcctt
     3601 cttcaaggac atcgccggag aagaggcgtt cgttggtttg cagacgcttg ttgcggcggc
     3661 tggttcagga gagctgcaaa aactgaccag agaattgcag ggggcaaggg gagaggccga
     3721 tcgcgtcgca aaagtaatgg ctgataatct tgatggggac ctgaaaaatc tcgacagcgc
     3781 atgggaaggt cttcgtattc gcatcagtga tctggttgac ggtccgctgc gttctgtcac
     3841 gcagtggctc acgcgggtgc ttgaaaaaat cacctcgctg gcgcaggccc atccggtact
     3901 gacgcgccag ctactgatag caggcggtgc gttgctggca atgactgcaa cgattggctc
     3961 gttgtcgctg gttattgggg tgctttacgg gaagctggcc accctgcgtc ttggttttga
     4021 cattcttacc cggtcaatga atgtcgtcag ggtgttacct gcgctatggg gaatggtgac
     4081 gggttccgtt tctttactgg gaggcgttat cggggcgttg ttcagtccgg ttggtcttat
     4141 cgtggctgcg cttgccggag ctgccgttct tatctggaaa tactgggatc ctatcagggc
     4201 tttctttgcc ggggtgttca gcgggattat ggaaaggctg accccgttgc gcgacacctt
     4261 tgaacggttt ggccctgttt ttgacgtaat cggaagcggt tcagccaggt gtttaactgg
     4321 tttaaatcgc tgctgtcacc gatggagtcc agcaaggaaa cgctggataa atgtaccagt
     4381 gctggcgagg tattcggtaa cgttcttggt ggtgcgttac agcttgttct gacgcctgca
     4441 aaaatgttgc tggatacgct ggcgtggata cttgaaaaac tcggtgtgct tccggatgaa
     4501 gcggaaaggg cgcgcaagaa aatcgaagac gcacaacgtg cggccattct tcaggacaag
     4561 gttgctctgt ttcagggaga cattgcgaaa atcaatccgc cgaagtctgc gggaaatagc
     4621 aatggcaccg gagatgataa acccaaagac aacaaacctc tcacagacag caataccggc
     4681 acgctacgca gactcagcaa aattgctgat aacacaggta agctggttga tgagacaaaa
     4741 aaacgcattg gccccggcga tattgtcttt aagaacctgc cccgcgcact tgctgttcgt
     4801 ggggagtggc aggagcggaa gattgcgcag gtcagtaagc ctgcccccgc aattaatatc
     4861 acacccgtgg tcccggctcc gctgcctccg gcgctggtcc ctgttgttgc ggccagctcc
     4921 cgcccggtgg tggaggccat acgatcgcca gtggcatcag ttcctgcaac ttcccgtaac
     4981 cgggagcctg ttgcctccgg atttggcggt gaaattcatg ttcatctgca taacgttgtt
     5041 acgcagaatc cccgcgaact ggcgaaactg gtcggcgaaa tggtcagggc agaaatggaa
     5101 cggcgcgccc gtgccaggcg tggcagtttt tacgataaag attgaggagt catggccatg
     5161 atgatgatct acggcatgtt tgtttttgag ctgcgcacgt tgccgcatca gcagttacag
     5221 caaaacaaaa gctggcgaca tgtgaaaaat gaacgcgtta accgttcagc aagctggcag
     5281 tatatcggtg caggtgatga tcgcatcgtt ctttctggtg ttctttatcc tgagattact
     5341 ggtggcgaag tgtcgctgtc gttgttgacc acgcaggcat atacaggacg tccttggcct
     5401 ttgattgatg gcgtcgggca gatttacggc atgtatgtcc tgaccggaac gaatacgacc
     5461 cgctccgagt ttgatcgcta cggtaaggcg aaaaagatag aattttcact gactcttgaa
     5521 cgctgtgatg aggatttgcg ggagcgcctg caatcctcat cgttcagcga tatgctgtcc
     5581 ggcttcaaag ataaggtgac atcatccctt aatagcgcgg ccagctcagt taaggggctg
     5641 ctctgattta acgtatgtcg ccaatttcct gatgaaggtg actggcgact tgctgttgta
     5701 tgtccttctc agaaaattgt ttttgaataa caaataacag gatt
//
LOCUS       DAZGXS010000209         5736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGXS010000209 DAZGXS010000000
VERSION     DAZGXS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..375)
                     /locus_tag="ACHKG5_004541"
     CDS             complement(<1..375)
                     /locus_tag="ACHKG5_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0870506.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKD"
     gene            complement(375..1844)
                     /locus_tag="ACHKG5_004542"
     CDS             complement(375..1844)
                     /locus_tag="ACHKG5_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0870507.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            complement(1911..4343)
                     /gene="hsdR"
                     /locus_tag="ACHKG5_004543"
     CDS             complement(1911..4343)
                     /gene="hsdR"
                     /locus_tag="ACHKG5_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP0870508.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            4621..4905
                     /locus_tag="ACHKG5_004544"
     CDS             4621..4905
                     /locus_tag="ACHKG5_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP0870509.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            4914..>5736
                     /locus_tag="ACHKG5_004545"
     CDS             4914..>5736
                     /locus_tag="ACHKG5_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP0870510.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQIKS"
BASE COUNT         1414 a         1279 c         1348 g         1695 t
ORIGIN      
        1 atctttatct ggagtttttt gtccccagtc atgtccaatt tcactaatac gcacccactc
       61 ccatccctcc ggcaattcaa acggcttctc ttcctcacta atttccggca gtggtttttg
      121 ctttttaatt ttaccttgct tcaccagctc tgctttttct gccgcaatac gcttcagcag
      181 ctccgacgcc ggttcatcgt tcgggtcctg cggcaccagt ttaccgcgca cagccagttc
      241 cagaatcagc tcacgtaatt tcttaatgcc atataaatca atttttccgg aactgccgcg
      301 cccggcggtg gaacgggttt gcaacgccga ggtccaggtt tccatatgat caacgatcag
      361 cttttccaca ctcattagtt aacctccttg ccagacagcg cagcgccaag aatatcgcgc
      421 agttgattac gcagcgtctg gatctccgcc tgctgttttg catactgcgc taacagctcg
      481 gccggatcat ggctgacggt ttccgcctga tgtgggtttt taatatccag attgaagtta
      541 cgcgcaatca cgtcatcaat gctgactttc cacgcctgct cattctctac gcggctggca
      601 aaaccatcgg cctcgttacc ccaccagtcg atctccgcct gaaactcttc aaacttcatc
      661 ggcttagttt tgctgtagtt ttttacgccc gccggatacg gatgctcata gaaccaaatc
      721 tctttggttg gctgaccttt ggtaaagaac aacaggttgg ttttaatgcc ggtatagggg
      781 ttaaacacac cattcggtaa gcgcacgatg gtatgcaggt tgcactcttc ggtaagtagc
      841 tttttgattt tggttttaac gccttcgcca aatagtgtgc catccggcaa taccaccgcc
      901 gcgcgaccat ttttcgccag tacttccaca atcagttgca ggaacaaatc cgccgtttcg
      961 cgggtttgca tctctgccgg aaagttcttc tcaataccgt cttcttccgt gccaccaaac
     1021 ggcgggttgg taacaatgac atccagttgc tcatcccggg aggaaagcgg tttgttcaga
     1081 gtgttgtcgt gacgaatttg cactggcact tcaatgccgt gtagcagcat attggtggtc
     1141 gccagcaggt gcggaagctg ttttttctca acaccgtgga tctgttgttg cagcgtctga
     1201 tgatcggcga cgctcttcac gtatttgttc tttacatgat caaatgcgca ggcaagaaaa
     1261 ccgcccgtac cgcaggccgg gtccataatg gattcgccga gtttcggatc aacgcgatcc
     1321 accataaagc gagtgacggc gcgtggagta tagaattcgc ccgcattgcc cgcagattgc
     1381 agatctttaa ggatctgttc gtaaatatca ccaaacagat ggcgttcgct ggcgctggta
     1441 aagtcaattt cgttcagctt attgatcacc tgacgcagta gcgtaccgtt tttcatatag
     1501 ttataggcat cgctgaacgc ctgcttcacc acgtagccgc gtgggttttt atcgataggc
     1561 gcagtgaggt ttttcaacgc cgggaacaga tcatcattaa cgaattccag cagggaatca
     1621 ccggtaatgc cctgcgcgtt ggcggcccaa ctgcgccata aataacgctg tgggatcgga
     1681 tattgatagt tatcctgctc cagttccagc gcctcttcct gggcatcgaa gattttcaaa
     1741 aacagcagcc aggagagctg accgagacgc tgcgcatcgc catccacacc ggcatctttg
     1801 cgcataatat cttgtaatga tttgattact gagctgatag acatgttgta ttccatgaaa
     1861 aacaaggcct tctgcaacat gatggttgca gaaggcgcaa aagagagttg ttaagcagag
     1921 cggggcggta attggtagat ttcgttttcc agctcgctga gcgcctgatt atacccgtta
     1981 cggtcgccaa atccggtttt aataatctcc ggtaaggtgc ccatgctgtc gaatggctta
     2041 agtttcagca cctgaatact ttcgatctcc tgtacgcccg catcggcgta tttatccagc
     2101 agattatcga gcacggcttg cgctgcttca gagtattttg tgaagtagtt ccgcttgcgc
     2161 acgttctcgg cgcgctcttt gcgggttaac ggcggctgac catacactac gtggcaaagc
     2221 atgtcgaacg ggtcgagatc tttaccgact tcttctgcca gtacttccca gatgatcccc
     2281 tgttgctcca gctcgtgaat gatcgcttct ttgcgatcgg cgtcctgcca cttgcgggta
     2341 aagtcatcca gcgaggcata ttctttgagc agtgttttgc gggtgtaatc tttaaaggat
     2401 tcggtaacca gtttaccgtc ggcgtcgtaa tactgaacac gctgggcaat aacgcccact
     2461 gctacaccgt tgacgtgaaa cttgcggatt ttcttctcgt cttcttccgg aagtgggccg
     2521 acatcatcgg tatctttaac ctggtatggt ggcgcaggcg gttcatcaac gcctgttacc
     2581 tgttcgtcgt catgttcgct gatttcttcg agtttctctt caaaatcaga ttctggatcg
     2641 gcgatatcct ctggtgtggt atccatgact ttttcgggaa tgccatcgaa acgctcatcg
     2701 gcaaacagtt cggtggcttt tttaaagtcg aggatggtaa accagagttt gccgtaacgt
     2761 tcgtcgatgc gtgtaccacg accgataatc tgcttgaatt tggtcatcga ctggatgttc
     2821 tggtccagta ctaccagttt gcaggtttta gcatccacac cggtggtcat cagctctgaa
     2881 gtggtcgcga taaccgggta cggttttttc gggttgatga agttatccaa ctgagctttg
     2941 ccaatttcat catcgccggt gattttcatg acgtatttgt cgttcttttt cacctgctcc
     3001 gggttgagat taaccagggc gcggcgcata cgttctgcat gatcgatgtc gttacagaag
     3061 acgatggttt tatccatcgg attggtacgc ttgaggtagt cggtaatggt tctggcaacc
     3121 agttctgtgc gttcgtcgat taccatcgtg cgatcgaaat ctttctggtt atagatacga
     3181 tcgtcgatca cttcgccgtt taagtcagtt tgccctttgg ttgggcgcca gccttgcaga
     3241 tcaacatcaa tatcaacacg gacaacttta taaggggcga ggaagccgtc ttcgatccct
     3301 tcttttagtg agtagacgta aaccggatcg ccgaagtaat ccgtgctgga gacttcatgc
     3361 gtctcttttg gcgtggcggt taagccaatt tgggtggcgg aactaaaata atcaaggatt
     3421 tctcgccagg cgctgtcttc ggatgcgctg ccgcgatggc attcgtcgat cacgatcaga
     3481 tcgaagaaat ctggtgcgac ttgtttaaac gctttttggt cttcctccgg gccagttata
     3541 gcctgataga gcgcgaggtg aatttcataa gcgggatcaa tggtgcgtcc gctgactttg
     3601 gtcattgccg taccaaatgg ctggaaatca ttatttttgg tttggtcgac cagaatattg
     3661 cgatcggcaa ggaacaaaat gcgtttttta ttttttgatt tccacaggcg ccagatgatc
     3721 tggaatgcgg tataggtttt ccctgttccg gtcgccatga ccagcagaac gcggttttgc
     3781 ccgttggaga cggcttcaat ggttttgttg attgcctgaa gctggtaata acgtggcgat
     3841 ttaccgctgc catcgtcgta atagtcctga gtaatcaccg gaagctgagc ttgtgtgtat
     3901 cctttccaga ggcagaattt ttgccagagt tcagcggggg aggggaagtc atccagcgtt
     3961 atttgctttt caaggcattc accttcggct gcggtggcat cgcggaagat aaagccatca
     4021 ccgttggtgg caaaaacaaa aggaacgtcc agcaggcgcg catattcaat gccctgttgc
     4081 atccctttgc caatttcatg tttgttggct tttgcttcaa tcacggctaa gggaatgcca
     4141 ggtttgtgat acagcacgat gtcagcagat tttaccgttc tgcgtgccgc aactttacca
     4201 cgtacgatga ctttaccgtc ccggagtttg acctcctgtc tgatttgcgt tgtgttgtcc
     4261 cagcctgcat tgagaatggc tggcataacg catttggtaa tgatgtctgc ttccgtcagg
     4321 ttacttaggt tcagttccgc catggggtat tctcgctttt atcaggacga ttctgattgt
     4381 acataaaatc aatagcctgg ctatgattta gggcggtgtt tcaacgtaaa gtacgtagca
     4441 gagatcacat tgatatctct gttatatctt gcttgaatta atctgggcat atcagagaca
     4501 gataaacaga agttacagga accatatcaa ctctaacaaa atatataaaa atcttatagt
     4561 tgcgtttctt tcgagcaaga aagataatgt tattgttgat tgtttgattg ggtatatgaa
     4621 atggaagcta aagaatgtaa ggtgcaggat atccttactg aaaacaaaaa gtttattatt
     4681 ccttcttatc agcgtcctta cagttggacg gtagataatg cagagcaact cattgatgac
     4741 atttataaaa gctctcaatc agaagagaat ggatatttta ttgggagtat gatttgtatc
     4801 aataaagggc aaaatcagta tgaagttgta gatggacaac aacgtcttac aacgttaagc
     4861 attattgtct ccgagctaaa aaaatcatcc cgattcaggg gataaaagat gatttgcaaa
     4921 aaagagttct gcctattgat gtttattctg atgaaacaga tgaaccaaga ctaattgtta
     4981 gaaaaaaaga gcatgatctt tataaatatt atatactgca ggattctaaa gattataaac
     5041 ctgaaaagcc atcggatact gagttagtat tcattagcaa cgctgaaaca attagagatt
     5101 atttgttgcg gttatctgtt gatgagttga agttgcttgc taaatatatt ttgcaaaatg
     5161 tatatatcgt atttgtgcaa acagatgatt tcgcatcatc atttagactc tttaatgtat
     5221 taaatagtcg aggtttacct ttaagtaatg cagatctttt aaaaaatgca ctgtttgaat
     5281 cagcttctac acataataaa aaatcagagc aaatagaatc agcatggtcg caaattgaag
     5341 atatggtcgg agtgagacgg cttgataaat ttcttactct tcataagctt tccgagaaaa
     5401 aagatcgtga cagggtgtta caaaaaggtt ttgaagcttt tattgaaaat ctccagcagc
     5461 aatttgatgg agatgctatt gctatgtcac tgatgttggt taattctgca aaaaattata
     5521 ctaagatttt agagaatgat tttgaacacc cttcaatacg acgaaaaata gcatcgctaa
     5581 gtaatttagg ggtagatgag tggattcctc cggttatggc attcatgaat cgaatggctc
     5641 gtacagaaga ttttaattta gatgattttt ctcaatttat taccgccttt gagaaggtat
     5701 atatgcatgg gtggttaaaa aaacaaatta aaagcc
//
LOCUS       DAZGXS010000210         5729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGXS010000210 DAZGXS010000000
VERSION     DAZGXS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..2636
                     /locus_tag="ACHKG5_004546"
     CDS             <1..2636
                     /locus_tag="ACHKG5_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0870511.1"
                     /translation="EDDVRPEALRRFELMVEEVARNASAVAQNTAAAKKSASDASTSA
                     REAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASKSAAAAESSKSA
                     AATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSARDASASKEAAKSSETSAA
                     SSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAAGSKTAAALSASAASTS
                     AGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAKTSETNAKASET
                     SAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTKATEAAGSATAA
                     AQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESLAATP
                     KAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKY
                     TAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSARKVNGKALSSD
                     ITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAG
                     ISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQW
                     DYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYA
                     LMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSAS
                     ASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNG
                     SVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVN
                     AAGNAENTVKNIAFNYIVRLA"
     gene            2636..3217
                     /locus_tag="ACHKG5_004547"
     CDS             2636..3217
                     /locus_tag="ACHKG5_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0870512.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3337..4227)
                     /locus_tag="ACHKG5_004548"
     CDS             complement(3337..4227)
                     /locus_tag="ACHKG5_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP0870513.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4246..4752)
                     /locus_tag="ACHKG5_004549"
     CDS             complement(4246..4752)
                     /locus_tag="ACHKG5_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0870514.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(4789..5289)
                     /locus_tag="ACHKG5_004550"
     CDS             complement(4789..5289)
                     /locus_tag="ACHKG5_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0870515.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(5368..5550)
                     /locus_tag="ACHKG5_004551"
     CDS             complement(5368..5550)
                     /locus_tag="ACHKG5_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0870516.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1545 a         1364 c         1373 g         1447 t
ORIGIN      
        1 ctgaggatga tgtccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg
       61 cgcgtaacgc gtccgcggtg gcacagaaca cggcagccgc gaagaagtca gccagcgatg
      121 ccagcacatc agcccgtgag gcggcaaccc atgcgactga tgctgcggac tcagcacgcg
      181 cagccagcac gtcagccgga caggccgcgt cgtcggctca gtcagcgtct tccagcgcag
      241 gaacggcatc aacaaaggct actgaagcat caaaaagtgc tgccgctgca gagtcctcaa
      301 aaagcgcggc ggctaccagt gccggtgcgg cgaaaacgtc agaaacgaat gcggcagtgt
      361 cacaacaatc agccgccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcct
      421 cctcagccag ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgagcgcag
      481 cctcgagcgc cagtagtgca gcctcctcgg caacggcggc aggcaattcc gcgaaggcgg
      541 ccaaaacgtc tgagacaaac gctaagtcct ctgaaacggc agcagaacag agtgcctccg
      601 cagcagcagg ctcaaaaaca gcggctgcat tatctgccag tgccgcgtca acaagtgccg
      661 ggcaggcctc agccagtgcc accgccgccg gaaaatcggc agaaagtgcc gcatcgtctg
      721 cttcaacagc cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgagtt
      781 ctgcttccgc agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc agcgcagaat
      841 cctcaaaaac ggctgccgca tcgtcagcca gttcggcggc gtcatcggca tcatctgcgt
      901 ctgcttcaaa agatgaggcg accagacaag cgtcagcagc gaagagcagc gccacgacgg
      961 catccacgaa ggcgacagag gcagctggta gtgcgacggc agcagctcag agcaaaagta
     1021 cggcggaatc tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat
     1081 ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg
     1141 cgactaacag cacttccgag tcactggcgg caacgccaaa agccgttaag gccgcgtatg
     1201 agctggctaa cgggaaatac accgcacagg atgcaacgac agcacagaaa gggatagttc
     1261 agcttagcaa cgcgaccaac agcacatctg aaatgctggc ggcaacgcca aagtcggtaa
     1321 aggcagccta tgaccttgct aacgggaaat atactgctca ggacgctacg acagcacaaa
     1381 aaggaattgt ccagctcagt agtgcaacca acagcgcatc tgaaacgctt gccgcgacac
     1441 cgaaagcagt gaaagcagct aatgataatg cgaatggtcg ggtaccttct gcccgtaagg
     1501 tgaatggtaa ggcgctttca tcggatataa cactgacgcc gaaagatatt ggtacgctta
     1561 actcaacaac aatgtcattc agcggtggtg ctggttggtt caaattagca acggtaacca
     1621 tgccacaggc gagttctgtt gtttcaatta cgttgattgg tggcgcggga tttaacgtgg
     1681 ggtcacctca acaggcaggt atatctgaac ttgttttgcg tgcaggtaat ggtaatccga
     1741 aggggattac tggtgcttta tggcagcgca catcgacagg gtttacaaat tttgcctggg
     1801 tcaatacatc tggtgatact tacgatattt acgttgcaat cggaaattat gcgactggtg
     1861 taaatattca atgggattat accagtaatg ccagcgtgac gattcatacg tcaccagcat
     1921 attctgctaa taagccggaa gggttaacgg acggtacagt ttattcactc tatacgccat
     1981 cagagcagtt ttatccgcct ggcgcaccaa tcccgtggcc atcagatacc gttccgtctg
     2041 gctatgccct gatgcagggg cagacttttg acaaatctgc atacccgaaa cttgcagccg
     2101 cttatccgtc aggcgtgatc cctgatatgc gtggctggac gattaagggc aaacctgcca
     2161 gtggtcgggc cgtattgtct caggaacagg acggcattaa atcgcacacc cacagcgcca
     2221 gcgcatccag tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat
     2281 ccacgaataa caccggggcg cacacgcaca gtgtgagcgg tacagccgca agtgccggaa
     2341 accatactca tagtgtcaca ggcgcatcag cagtcagcca gtggtcacaa aatgggtcag
     2401 tacataaggt agtgtctgcg gccagtgtga atacaagtgc tgcaggagcg cacactcata
     2461 gtgtcagcgg cacagctgca tctgcaggtg ctcacgcaca tactgtcggt attggtgctc
     2521 atacgcactc tgttgcgatt ggctcacatg gacacaccat caccgttaac gctgcgggta
     2581 acgcggaaaa cactgtcaaa aacatcgcat ttaactacat tgtgaggctt gcataatggc
     2641 attcagaatg agtgaacaac cacggaccat aaaaatttat aatctgctga ccggaactaa
     2701 tgaatttatt ggtgaaggtg acgcatatat tccgcctcat acaggtctgc cagcaaacag
     2761 tacctatatt gcaccgccag atattccggc tggcttcgtg gctgttttca acagtgatga
     2821 gtcatcgtgg catctcgttg aagatcatcg gggtaaaacg gtttatgacg tggcttccgg
     2881 cgacgcgtta tttatttctg aactcggctc attaccggaa aatgttacct ggttatcgcc
     2941 ggaaggggag tttcagaagt ggaacggcac agcctgggtg aaagatgcag aagcagaaaa
     3001 actgttccgg atccgggagg cggaagaaac aaaaaacagc ctgatgcagg tagccagtga
     3061 gcatattgcg ccacttcagg atgctgtaga tctggaaatc gcaacggagg aagaaacctc
     3121 attgctggaa gcctggaaaa agtatcgggt gttgctgaac cgtgttgata catcaactgc
     3181 acctgatatt gagtggcctg taatacctga agtttaaaat aaaagcccgc ttaaaatatc
     3241 gcgggcttcg aaatataaat gtattttcag aggctagagc ttaggatatc tcagcggcaa
     3301 ttaacttatg agggatagct aaataccaga agataattag tttttactat ttatctttgt
     3361 ttgtggttct ccttcagcaa gctcagcgcc agtgacagga ttgatgtctt catgggattt
     3421 caaccttgtt gccatagctt ttatcatagc ttcttgcttg gtcgaaagat ttatcgaggc
     3481 cttcccatcc ccaccgtcaa ccgctgaaac tggatcggaa acatagtcaa agttttcatc
     3541 actgttccaa ctacctctga cgtcttcacc ttctgacatg ttgtaatata cgtttttgta
     3601 tttctcaagt ggtggtaatt ttcctggtgg aaaggtgtta cgaattgaat gtaatgcttt
     3661 ttcaaatgaa agcatgtgtg ctgcttcccg ggtcattaaa aatgccagag tgtcttttac
     3721 tccaggatca tcagtaacat taatgagacg ttcgtaaatg atctttgccc gagcttcagc
     3781 tgcaatattt gaacgaagat cggccgtgac ttcgccaata gtatcaacat aagctgcagt
     3841 ccagggtact ccagctgaat tagttaatgc tgggcctcct ccgtagagga gagaagttat
     3901 atggctatca ttgccatttt gagttaagga acggtaaagc tcagcttcat tctcagttcc
     3961 ttcagcaagt gctccttttg cacctttgtt gagcatacca acaagagaac caataatttc
     4021 gagatgactt agttcttctg ttgctatgtc catcagcata tcccttcggc ctgcatcttc
     4081 atcacttaag ccttgagtga agtatcggca tgctgctgca agctcaccct gtggcccacc
     4141 gaattgttct aagagtagat tagccaagcc tgggtttggc tcacttacac gtactgtata
     4201 ttgaagtttt ttcacgtgtc taaacataat gcctcctcaa aagaattatt ttttggcttc
     4261 aactccatca atttcagatc tgaccatgaa ttgctcagtg gtgtcaggaa tatggttaag
     4321 aagccattca gccatttgtt tttcttcatt aagaattgct tcaataatgg gtactgaagc
     4381 tgtatcaccc gcatttttgg ctgctgcgag aagagatgtg taacacgcaa tttcaaattg
     4441 ttcgaacaca tagccactga tcgaaccttt gacaatttca tcagatggaa aaattccacc
     4501 aatggattgt ccaaacgcgg ccattttact catagagtct ttaatgacag agcgcgaaat
     4561 attattacga tcaagtatgc tttcaagctg tgaaagctgg ttctttgttt cgctaatgtg
     4621 ctgctcaatt ctggaacgta actcaggata gttttctatc cggctggcca tagactcaag
     4681 cattttttca gcttgttttt ccatcgcatg tgcatcacga agccagtcat ggtaatgttc
     4741 aattctattc ataatctgtt tatcctaaaa gaaattgaag tctatagttc aggagttatc
     4801 ctgagctttt ttattaacgt tattaaatgc caggtctgtt aatttgacat cagtagcttt
     4861 ttcctcttcc agtgtttctt tcaggagttt agccgctttt ttatatccta attgctcagc
     4921 gagtgttacc aaagtgccat aactggcaat ttcatagtgc tctacttttt gtgctgcagc
     4981 aatcaatgca gcatcacgta cttcattttt atcagtgctt tcaattacct cattcgcctc
     5041 ttcgataagt ccttccattg cggcacactt aattcttttc aatttaagac catcttcaga
     5101 atcaacaacc tgatcgatgc gttcaatttg accatgtgtc tcatcaagat gtgactgaaa
     5161 agcagcggtt aatttatcac tatatgcaga gcgactaagt ttagacagtg cctttgttag
     5221 ttgtttctca gcgctgtatg tatccgataa taagtggatg aagatatctt caacagattt
     5281 aatttgcata atcactccag agttttcact gcgagcccgt aggctcgcag ttagcttcag
     5341 ttattcacga atgtaggggg tgataaatta agagtcagat ttacggccgc caccatggct
     5401 attctgacca ccttttttcc ctgcttctga tgctctttgt ggatcgtttt taaaattacc
     5461 gccactatgt tggccgcctt tacgaccagc gtcagaagct ttttgtcgat cttcagcgaa
     5521 attacctgca ccgccacgat gttcggtcat atcatttctc ctctgatttt caaattgtaa
     5581 ataacatgtg ccttgtcagc acccaattaa ctctagtaac aaataagaaa gtgtcaaata
     5641 agagattggt atgcgcatct taatgtgaga aatagcaatt gtaaaaatgt gtactcagtg
     5701 agtataatct gttgatatct tggtgatta
//
LOCUS       DAZGXS010000211         5569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGXS010000211 DAZGXS010000000
VERSION     DAZGXS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1222
                     /locus_tag="ACHKG5_004552"
     CDS             <1..1222
                     /locus_tag="ACHKG5_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFP0870517.1"
                     /translation="AASETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDV
                     DENNLKVFNVHFDNNEVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSAN
                     KGEIEQLVEWSKRNEQTLFDNIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYEN
                     KIDEVSSLSESEEHPIDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRA
                     QPQDKIDSYNSALQAFSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDV
                     REIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIF
                     NTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPED
                     APIENSPVVNSPLV"
     gene            1592..1750
                     /locus_tag="ACHKG5_004553"
                     /pseudo
     CDS             1592..1750
                     /locus_tag="ACHKG5_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1974..2093
                     /locus_tag="ACHKG5_004554"
     CDS             1974..2093
                     /locus_tag="ACHKG5_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0870518.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            2158..>5569
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004555"
     CDS             2158..>5569
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP0870519.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDA"
BASE COUNT         1698 a         1089 c         1380 g         1402 t
ORIGIN      
        1 agctgcatca gagacgtatc gcggtgctct tgaaatttta caaaaaataa tgtctgaatg
       61 tatatatcaa gaaggcaacg cctttgtcat tatgggagct ggggaacaat taaaacgtat
      121 taaatatgat gttgatgaaa ataacttaaa ggtattcaac gtacactttg ataataatga
      181 agtgttagtt actgatggtg agcctgacgt agtatgttta agcaagcagg tctgggaaaa
      241 tcttctcatt aaattaaaac cggagatcaa ggaaaatgtg gcttctgaag ttcataaatc
      301 agcgaataaa ggtgagattg agcaattagt tgaatggtct aaaagaaatg aacaaaccct
      361 tttcgataat ataataaaaa gtgattttca tgttggttca cttaagccag gtagtatgaa
      421 tggtgttatt ttagaaatgc caccaaatgt ctgtatggaa ccacgtaatt catatgaaaa
      481 caaaatagat gaggtttcat ctttgtcaga gtcagaggaa caccccatag atattcaaaa
      541 aataacagat gcgtttgtga aggagttcaa ggggatatta tttgataaaa atggaagatc
      601 ttcagagctt ctgtttaatt tttatgaatg ttgctatacg tttttaccaa gagcgcagcc
      661 tcaagataaa atcgatagct ataattcagc actgcaagct ttttccatct tttgttcatc
      721 tacgttgaca cataataatg taggctttga tttcaaatta tttccagaag tcaagctgtc
      781 tggagaacat cttgaaacgg tattcaaata caaaaatggc gatgatgtcc gggagatagc
      841 caaaattaac attactctcc aaaaagaaga gggtggctta tataatctac gtggattgga
      901 ttttaaggga tgcttctttt ctggacagaa cttcagtaac tatgatattc aatatgtgaa
      961 ctggggaatg tcattgtttg atgttgatac tccgtgtatt tttaatacgc ctgctaacca
     1021 tgagagttat gaaaaatcat taaaacctgt aagcgaaaac ggtttaaatg gagtcttatc
     1081 tgatcgtaat aaaaaaataa aaatgatcac gggtgtggca ccattcgatg atattttatt
     1141 tatggatgat gactttgatg ataactcccc tgaggatgct cccattgaga atagtcctgt
     1201 tgtgaatagt cctcttgtat aaagttctaa aagcagtttt caatgataaa taaatggcaa
     1261 tttttataaa gagataataa cgtagattga aacaatatgt attattgatt gagtaaacgg
     1321 gattattctt cacactacat ccattttagt gtcactacca aagaaaaagg ggctacgttt
     1381 tcatgtaacc ccttgattta tttggtgggc tggcagtgac tgaattggtg ctgtaattgg
     1441 ttgaaatatt tcatctattg tctggttcaa ctttccttag ctgcctgaaa gttttttgat
     1501 ctcacccagc aataacagcg ttacggtgca ccacgcctga ctgaagaact gcgtgctcag
     1561 ggttaccgct tcaacgtaaa accgtggcag gaggtccgga ctgaaaaaac tgctcaggac
     1621 atcatcggca gggaaagcac tgctagtctg taaactgaac cgactggggc gcagtatgtg
     1681 gcatcttgtc gtgctgttgg aggagctgtg cgaacgtggt attaactttc gtgctctggc
     1741 ccaatctata tttgcccaac aatgggggga cgaatgctgt aaaagtaaaa caatctgcga
     1801 tctcaaagtt attgtgtgat ttttatgtga gcagaagata ttcatcagaa acgattatgt
     1861 aaatcatttt attttgccaa cggcctcatt gtcgaaagaa aagaatacgc ccgtatttcc
     1921 tgaatggatt gactttttta tccaaccaca cttcagcgca ctgcgtttaa aaaatgcctc
     1981 attcttatgc ggaatatcat catttcatca tgatgtcttt gatgagcggt gaacacaata
     2041 cacttgcgct gtctcttcag gatgaatccc ctggtctggt gcctgtgggc tgatgttgca
     2101 gcaaagctaa ggtcgcttaa acgctactca gtattcactt ttcagaggat gaaatttatg
     2161 aacaggacca gtccctatta ttgtcgtcgc tcagtacttt ccttattgat atctgccttg
     2221 atatatgccc cgcccgggat ggctgccttc actcctgatg ttattggtgt ggtaaacgat
     2281 gagactgtag atggcagcca acgagtagat gaacgaggta caacaaataa cactcatatt
     2341 atcaaccatg gccagcagaa tgtttatggc ggggtatcta atggaagtct tattgaatct
     2401 ggtggatatc aagatgtagg aaggcataac aattatgtgg ggcagtctaa taataccacc
     2461 attaacgggg gcagacagtc aattcatgac gggggtattt ccacaggtac gataatcgag
     2521 agtggcaatc aggacgttta taaagggggt atcagcaatg gaacgacaat taagggcggt
     2581 gcttcacgcg tagagggagg gagtgcgaat ggaacactca ttgatggtgg tagccagata
     2641 gtaaaagttc aagggcatgc tgatggtaca acgataaata agtctggctc tcaggacgta
     2701 gtacaaggaa gtctggcaac gaacacaacc ataaatggtg gtcgacagta tgttgaacag
     2761 agcacagtag aaacaaccac catcaaaaat ggcggtgagc aaagagtata tgagagccgt
     2821 gcgctggaca cgacgattga aggcggaact cagtctctga atagtaagtc aacggcaaaa
     2881 aatactcaga tctattctgg tggtacgcaa attattgata acaccagctc ctcggatgtt
     2941 attgaagttt attccggtgg cgtgcttgat gttagtggtg gtacggcaac aaatgttacc
     3001 cagcacgatg gtgcaatttt aaaaactaac actaacggta cgacggtgag cggtacgaat
     3061 agtgaaggtg cattctccat ccacaatcac gtggcagaca atgtgttgct ggaaaacggt
     3121 ggtcatttag acataaacgc atatggttcg gcaaacaaga cgattattaa agataaagga
     3181 acaatgtcag ttttaaccaa tgctaaagct gatgcgaccc gaatagataa tggcggggtt
     3241 atggatgttg caggaaacgc gacaaatacc ataattaatg gtggcacaca gaatattaat
     3301 aattatggca tagccacagg caccaatatc aacagcggaa cgcaaaatat caaaagcggc
     3361 gggaaagctg acacaacaat tatatcctcc gggagccggc aggttgttga gaaagatggt
     3421 acggcaattg gcagcaatat tagcgccgga ggctcgctga ttgtctatac cggcggtatt
     3481 gcacatgggg ttaaccagga gacgggcagt gctttagttg ccaacacggg tgcagggact
     3541 gatatcgaag gatacaacaa gctctctcac ttcactatta ccggagggga ggctaattat
     3601 gttgtgctgg aaaataccgg cgaactgacg gtagtggcta aaacctcggc gaaaaatact
     3661 accattgatg ctggcggtaa gctgattgtc cagaaggagg ctaaaacaga tagcaccaga
     3721 cttaataatg gcggcgttct ggaggttcag gacggtggtg aggctaagca tgttgagcaa
     3781 caatccggcg gcgcattaat tgcttccacg acctccggaa cacttatcga aggaaccaac
     3841 agttatggtg atgctttcta catcaggaat tcagaagcta aaaatgtagt gctggaaaac
     3901 gctggctcat taacagtcgt cactggttcc cgggcagttg acacgattat taatgccaac
     3961 ggcaaaatgg atgtttatgg aaaagatgtt ggcactgtac tcaatagtgc tggcacccaa
     4021 acaatatatg ccagtgccac ttctgataaa gcaaatatca aaggtggcaa gcaaacggta
     4081 tatggtttag ccactgaagc aaatatcgaa agtggtgaac aaattgttga tggtgggtca
     4141 acagagaaaa cacacatcaa tggtggcacg caaaccgttc agaattatgg taaggcgatc
     4201 aataccgata tcgtctctgg cctacaacaa attatggcaa acgggacagc ggaaggttcc
     4261 attattaatg gcggttcaca gatagttaat gagggcggtc tggctgaaaa ctcggtgctt
     4321 aatgatggcg gcacactcga tgtgcgggag aaaggcagcg caacggggat acagcagagt
     4381 agccagggcg cgttggttgc aaccaccagg gcgacgcggg tcacaggaac acgcgcggat
     4441 ggcgtcgcgt tcagcatcga gcagggtgcg gcgaacaata tcctgctggc aaatggcgga
     4501 gtgttaaccg tggagtcaga cacctcttct gacaaaacac aggtcaatac gggcggacgg
     4561 gagatcgtca aaacaaaagc cactgcgaca ggcacgacgc tcaccggcgg tgaacaaatt
     4621 gtcgagggtg tggcgaatga gacaacaatt aacgacggcg gaatacaaac agtttcagct
     4681 aacggagagg caataaaaac aacgatcaat gaaggcggta cgctgacagt caacgataat
     4741 ggcaaagcga cagatatcgt ccagaacagc ggtgccgctc tccagacgag cacggctaac
     4801 ggtattgaaa tcagcggtac tcaccagtac ggcacttttt ccatttccgg caatttagcg
     4861 accaatatgt tgctggaaaa tggcggtaat ttattggtat tagcaggtac cgaagctcgc
     4921 gactccacgg ttggcaaggg gggggcaatg caaaaccagg gtcaggactc cgccacaaag
     4981 gttaactctg gtgggcaata tacccttggg cggtcaaaag atgagtttca ggctctggcc
     5041 cgggcagaag atctccaggt tgctggcggg acagcaatcg tctacgcagg tacgctggcg
     5101 gatgcatcgg tcagtggcgc gacaggaagc ctgtcgttaa tgacgccacg ggataatgtt
     5161 acgccagtta aactcgaagg ggcgatccgg attaccgata gcgcgacatt aactatcggc
     5221 aatggcgttg atacgacgct tgccgacctg acggctgcca gccggggcag tgtctggctt
     5281 aacagcaata attcctgtgc aggcaccagc aactgcgagt atagagtaaa cagtttgcta
     5341 cttaacgacg gtaatgttta tttatcagca caaacagcag cgcctgccac aactaacggt
     5401 atatacaata cgctgacaac caatgaactt tccggtagcg gtaatttcta cctgcatacc
     5461 aacgttgcag gctctcgggg cgatcaactg gtcgtcaaca acaacgccac tggtaatttt
     5521 aaaatctttg ttcaggatac cggcgtcagt cctcagtctg acgacgcga
//
LOCUS       DAZGXS010000212         5491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGXS010000212 DAZGXS010000000
VERSION     DAZGXS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            283..474
                     /locus_tag="ACHKG5_004556"
     CDS             283..474
                     /locus_tag="ACHKG5_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0870520.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(527..760)
                     /locus_tag="ACHKG5_004557"
     CDS             complement(527..760)
                     /locus_tag="ACHKG5_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870521.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(856..1479)
                     /locus_tag="ACHKG5_004558"
     CDS             complement(856..1479)
                     /locus_tag="ACHKG5_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870522.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1568..2077)
                     /locus_tag="ACHKG5_004559"
     CDS             complement(1568..2077)
                     /locus_tag="ACHKG5_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870523.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2535..2993
                     /locus_tag="ACHKG5_004560"
     CDS             2535..2993
                     /locus_tag="ACHKG5_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870524.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            3231..3928
                     /locus_tag="ACHKG5_004561"
     CDS             join(3231..3480,3480..3928)
                     /locus_tag="ACHKG5_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFP0870525.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            3945..4205
                     /locus_tag="ACHKG5_004562"
                     /pseudo
     CDS             3945..4205
                     /locus_tag="ACHKG5_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(4347..5471)
                     /locus_tag="ACHKG5_004563"
     CDS             complement(4347..5471)
                     /locus_tag="ACHKG5_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP0870526.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT         1604 a         1288 c         1134 g         1465 t
ORIGIN      
        1 gaaaattatt ccactgaatg cttttaatac agttggttag cttaaaaaat agcaattttt
       61 gatttcagtc attgaaagtt gcaatgtttg acgattcaac agcatttaag tgagtttcag
      121 gatatgggaa ggacatgaaa ggaggagcgc aataaaatac acaggaagaa ggggaaaatg
      181 tttcgtaaaa acagaaaatt aacagcgcac atcaggaaac gcagtctcct gattcagtgc
      241 taccacatga aaatttagtt aactgttacc gtactaaaat ttatgtcaga agatgttcca
      301 ttaccgaaag tcaatcagcg atacaaagac gatcatggtg cgcttgtcac cctgaccagt
      361 gttgaggaaa cccgggtggt gtttatgcag gacggctatc cgcatccctg catgcgaccg
      421 atgtacaact ttctgggaaa attcaaaccc gaaccccggg aagaaacaga ataaaacgaa
      481 atccctgccg gcaatcacac tacctgatag cagcataatc cacttattaa ttctcatccc
      541 cggagacaag cccagaaaac cactccgggt gttcactgta tgccgtgttc agcgcatccc
      601 tggaaacgtt tatataaaag ctgtcctgca atttgcgggt aacaacttct gccaggcgat
      661 tccggttatc caccagtccg gcataacctc tctctgccag ccactcattc agttcatgtt
      721 ccagagacag attgataata tcataatcgg accttgccat tcccgtttcc ttagcacatg
      781 ttttcaggca cggggataaa gtataaagaa tgtcatactg ctgctgccag agcaatattc
      841 cccctgatgg ttgtatcaga gatggtatgc cggtactttg cgggttgttc ccgtcaggtt
      901 atcgtgcacc tccacggtcg tggtcaccac cggcattccg gcctttctca gcctcagaac
      961 atcagccgca atacgctgac tgccaaacct gaacaggccg tccagtgcag tcaccagtaa
     1021 acccgcctcc agtgcatact tcagccgttc acggggcgct ttcacttccc gggcaacctg
     1081 ctgatatggc aatgatgtgt tttcattccc aaccacgcga cgaatacggt gagacagatg
     1141 ataccggtat gtatccggca caccagaaag gctggccttc aggctgtaca cgcagccaaa
     1201 ccgtttatcg ttaaacacca catttttctg gctgatgccc cattcttcac gcagcgcggc
     1261 aatcaattgc ggtgtacggg taagcagcaa gcgaaaaggc agttcaaaac tggtgacgta
     1321 atccacattc agcagtgcaa tacgcaaccg ttcttctggc ccggcctctg tctgccggca
     1381 ctcctccaga acatcctgcc actgccggcg aagacggggg gattcgttcc gttctgtaaa
     1441 acagtattta tccgccagat agtcaattcg tgtacgcata ctgaaaggaa ttccgtataa
     1501 agattcagca gacaaaactt tatcagccag taaaaattaa cggaagcatt gatattcctc
     1561 ccgacaatca ccggatgatt gttgcaataa cctgcgacat aaaggactga acagccgttt
     1621 ttaacgccac gtattgccct gatgaaccag gccgggccac ccgaaaacat ctttccaccc
     1681 ggcattgtcc gccagcactt atcaccgacc aggtctgttg cagtaaatcc accagttgcg
     1741 cacattcttc atcagccatg acgccgactc gcggatggca aatactcgtc tggctgagca
     1801 gcaacagaca tcgtatctgt gctccttcat gttgttcccg caaccagcgt aataccaccg
     1861 gcgctgatgg gctgaccgtg tgattatgct ccgtaatacg ttcgtgtacc cgtcggtgaa
     1921 aggaactaca aaatgtctga atctgttgac catagattcc ttctgccgaa tagagcgtga
     1981 agtgaaatgc caacagattt ggccagtggt ccaccgcctg ctgaacaaaa cgccggattt
     2041 cccccggctc tgaaattaag gcttcggtta tttgcacgaa atgttccttt tcaacccctg
     2101 ttgaattttg tcaagccggt gcagacacag taaagcgcgc atcaacacct gaaaggtacg
     2161 atacaaacac aagttacaaa caataagaac ggaaggacaa ttaatcagtg aggaataata
     2221 actaaattaa ccaaaagaat gttactgaca atactaccaa tacttaaata gcaatatatt
     2281 aattaatgta agaacagatg cccggtagga catatacaca atacatccac aaacacactc
     2341 ttttcattaa atcaatcacc tgattaaacc atgaaagtgt gatgtaagtc aaaattactg
     2401 atactatcaa ttgataatga ttatctctat cattatcgtg aaattcgaga atgaaaggag
     2461 aagtagtcat tataagcctt ttataaatcg tcatcatcat agtaaagcag aacacttttt
     2521 atatgagtta ctatatgaaa atatactgga caagaaaacg tattcctgaa ctttcagcac
     2581 ttccaccttc attaagaaag aaaaatttta ctgacgcata taatgcagca tcttctcata
     2641 tcgaatactg gataggtgct ggcgtctcat ttatctcgat gatgatttta tccagagtat
     2701 atgactttct tttacccgcc caagatactt tcccgggtga tatcatcaga agtttatgtg
     2761 ttgtgtgtcc atccatatta atctggtttc agttttcggt atatgtcatg aggaaatatt
     2821 acagacatat tctggtgcga ggaaaagaaa ccgaaaccat ttcagaaagg ttgatacgtg
     2881 aggctgatac acgagaatat gaactatggc gtccggtccg tcgctttttc tccattgttt
     2941 tcctcattgt tctattaggt tgcattcatt cactgataac aactataaaa taaaagttat
     3001 ggctgttctg atacaggaaa ataaacgttc attctatggt tacctcttcc tgttatatag
     3061 aagaatttta aacgacaagt aagggaatat aaatgaaaat aacaaaaaat aaaaatgcaa
     3121 ctataaaaaa cagtcatcag tcggctgctt ttcaatgttt accttatcat tttctggtaa
     3181 tgctgccaac ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggcttcca
     3241 tttccatcag atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca
     3301 ctgccggaca tcagcgctat ctctgctctc cttgccgtaa aacatggcaa ctacagttca
     3361 cttacaccgc ctctcagccc ggtaagcacc agaaaatcat tgatatggcc atgaatggcg
     3421 tcggatgtcg cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact
     3481 taaaaaactc aggccgcagt cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt
     3541 ctgcgctgaa atggacgaac agtggggcta cgtcggtgct aaatcacgtc agcgctggct
     3601 gttttacgcg tatgacagga tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac
     3661 tctggccaca ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatggat
     3721 gacggatggc tggccgctgt atgaatcacg cctgaaggga aagctgcacg ttatcagcaa
     3781 gcgttacact cagcgcattg agcgacataa tctgaatctg agacaacatc tggcaaggct
     3841 gggacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
     3901 ttatctgaac ataaaacact atcagtaagt tggagtcatt acctgagaat aatacctcct
     3961 gggtaagtga gcagatactt tcagcgcctc cgaaagatgt aaatattcga cttggagtcg
     4021 gttctttaga aggtacaacc gtctcacatg ttcagcgatt gcatcattcg ttaatcgcag
     4081 ctggtttgga aagtaacctc actgtctata ccggtggtca cgattatgcc tggtggcgcg
     4141 gagcaattat tgatgaatta gcaaattata attgcaggaa ggtatcaaat aataactctg
     4201 tgtaaaatat cgttttatcg tcagataact ctttctgcaa tattattatg ggagactatc
     4261 tcatttattt agtgatgtcc ttcatttatg tgatgaagga cattatatta ctcataacat
     4321 ttctcgttga aaatttcagc aatacgctac aattgtttcc cgttgttttt taattttctc
     4381 atcaaaacct ctgcaatctc tgcaggactg ggttgttcag ccatttcagc ggctacctga
     4441 tctgaacaga agcgtagtga atcatcataa agaaggcgat ttaccaaatc actggtcagt
     4501 ccatgcttgt ccggaataat cccacaccca cgcttcgcaa caatttctgc attaacagag
     4561 cgatctgcac cttcgccaaa cacaatctgt ggtattccac tatacagcgc tgtcagggta
     4621 ttacctgcgc caccgtgatg aataaatcca tcagctccat taaggaatac acccataggt
     4681 atccagtcaa ccagacgtac atttgatggt agttttcgta aaccagtacg agcatttatt
     4741 gcaagttgca aaatgatatc agcatcaact tcatttgcag aatccataac ccatgaaatc
     4801 agctccagac catcaaccat tggttttaca gtgccaaggc tgataagtaa acgtttccga
     4861 tcagaatccc tgtcccacca tgtttccttt acagcacctc cgttataagg aatatatctc
     4921 attgagataa ccggttctcc agcattttta agaatgctca tacttggggg agcaacatca
     4981 atccatgcta aatcacatag tggaccgctg actccatgcc gcctgtaagc attgcttaaa
     5041 gaacggttta acatctgaat atgggcagat gtatgcgcga atccaacagc cagcatcact
     5101 gaaggaattc tatatttagc agcaaccaat gggcctgccg gatcaagagg gggatagact
     5161 attaaatctg gcctccattt tcctgcaaaa tcgatgaggt tatctgccat ttcatcgcta
     5221 aaaaaagaga aatgaccaat aatattactt tttttcctca actcttcctg atggcgatat
     5281 ccagcctctg aatctaaacc tggaactgca tcaaaaacaa ccagtccagc ttctgctgct
     5341 ttattcgcga atttgccagc gctactaatt actacatcat gtccaattac acgaaaggcc
     5401 tgagccagag aaatcaatgg gtataacaaa ccgtacagcg gtgggccaat aaaaagaata
     5461 cgttttcgca ttaaaaaagt ccgtcttctg c
//
LOCUS       DAZGXS010000213         5256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGXS010000213 DAZGXS010000000
VERSION     DAZGXS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHKG5_004564"
     CDS             complement(<1..206)
                     /locus_tag="ACHKG5_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0870527.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDA"
     gene            complement(190..432)
                     /locus_tag="ACHKG5_004565"
     CDS             complement(190..432)
                     /locus_tag="ACHKG5_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0870528.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            824..1162
                     /locus_tag="ACHKG5_004566"
     CDS             824..1162
                     /locus_tag="ACHKG5_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0870529.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1455..1607
                     /locus_tag="ACHKG5_004567"
     CDS             1455..1607
                     /locus_tag="ACHKG5_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0870530.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            1619..2257
                     /locus_tag="ACHKG5_004568"
     CDS             1619..2257
                     /locus_tag="ACHKG5_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870531.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2258..2467)
                     /locus_tag="ACHKG5_004569"
     CDS             complement(2258..2467)
                     /locus_tag="ACHKG5_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870532.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            3032..3220
                     /gene="dicB"
                     /locus_tag="ACHKG5_004570"
     CDS             3032..3220
                     /gene="dicB"
                     /locus_tag="ACHKG5_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0870533.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            3217..3405
                     /locus_tag="ACHKG5_004571"
     CDS             3217..3405
                     /locus_tag="ACHKG5_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0870534.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            3498..4736
                     /locus_tag="ACHKG5_004572"
                     /pseudo
     CDS             3498..4736
                     /locus_tag="ACHKG5_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            4724..5256
                     /locus_tag="ACHKG5_004573"
                     /pseudo
     CDS             4724..5256
                     /locus_tag="ACHKG5_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1535 a         1192 c         1202 g         1327 t
ORIGIN      
        1 gcatcagggg tgtctttctc tatccagcgg aaaattttct gggtattgcg agccagggct
       61 tccggatggc tgtcgtcgta cagttcagga aacgtcatac ccaactcaaa ataagcctgg
      121 gttattccag ctgccggaac tttttcgcca tcaggacgcg cccaggcatt catcgccatg
      181 cggatgtgtt catgcttgat tttcatgaat ccccccttgg ttagaaggcg gattatgatc
      241 agaatcggga atgacaaccg tcggtatgtg taactcatat ttgagcgccc cggcagtgac
      301 tgcctgaatt agcaacgccc atttccacgg aacgtcttcc ccccacatgc tgactgtggt
      361 ttttgacgtt cctagagcgg cggctgtttt aacaactccg ccaaaatagc ctaatacttc
      421 tgattttttc atgagtcgct ccataaaact gaacgccaaa agtttaataa tcaaaaccaa
      481 agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat
      541 atctgaacgc ataacccaac gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa
      601 tgccactggc gcatcaaaag gctccgtaag tcaatggatg aacggtggag gagcgccgtc
      661 ctcgcgttac ataagttcac tggcaaagat attgaaagta aacgaaaatt ggcttcttaa
      721 tggaggagag ttaaatacag gtgattcgct tgatctatct ttaccgccga taaaaacggt
      781 tccgctacta tcacttcagc aggcagcaag ctggagtgat tatatgaaaa attcctcaat
      841 aacctcttgt gtgcagcttg tcggagaaat cccggccaat acctttgctg ttgttctaga
      901 gagtgacagt atgtcgacat ctggtggtgg agtttccatc ccaaatggtt caacagtttt
      961 tgttgatccc gatcgaatcg tacaaccagg aaatattgtc cttgccttac ccaaagggac
     1021 cacaacgcct gtcattcgca aactggagat agaagggccg gatattcttt tagtccccac
     1081 gaatcctcgc tacccttcaa ttatgctgga tgatctatct tgcatattgg gagtatgctt
     1141 taaaattcaa caagatattt aaccaacctc atctatttga ttaactgtat gccatcgtag
     1201 tgatggcgta acagctgcct gcttaaaatg ttttgataaa aaaacattga ccttaaatgt
     1261 tcgtttttct aaacttcatt cattccctca ccccgcccca cagaatgcag ggcaatactt
     1321 cgagttacca ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
     1381 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
     1441 gaaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg
     1501 tgttccccaa ccaggacggt acgttcaccg cgatgacgta taccaaaagc aaaacgttta
     1561 aaactgaagc tggcgcgcgt cgctggttag ccagaaacac tgactgatga ggttgacgat
     1621 ggaatttaaa gatttacctc cttcaatcca ggagattgca gcacacacac ttcgtcatcg
     1681 tctgaacgaa cttgaattgg aatcggtaac gaaaaaagac actgataata tggctcgtaa
     1741 tgtgcgcgat gcgtttaccg gattgtattt ctgtgcgtct ataaataaac acgactcaga
     1801 gagtgtggca aataaaattg cagaaacgac agcgcaaaac atcaatacga aaccaacgga
     1861 agaagaaatt gatcagtttg ctcatgatgc tggtttaaaa aacaagaaag aaaaatcgcc
     1921 atatgcgggg aacatgtttg tttatgacaa tctcatcaga attcgtggcg aaattccggc
     1981 ggaatacctg gcaagagtcc atcaggcatt gcttaaaaat ttggaaacag aattatttga
     2041 tggcaacact aacggtttct tcatggtatc aggccttgag aaagactggg atgcagaaaa
     2101 acgctggaat gttgctacat ggttattcag taacagagcc gctgcactgg aagcttcggc
     2161 atgtatttgc ggcctgttct taacagacca caaatataat ctggatgtgt acagttatat
     2221 ttacgctgaa cacggtccac tctggattga ctggtaatta taaggaaaca ccagcagggc
     2281 cgcggcgacc aacagaacga ttaaaatcaa taatgccatt ataaaggaca ttatttaatt
     2341 tatcgtcgaa tgctgattct gtgagcctca actctgaatg agtttttaat aaccctgatt
     2401 gcctgagttg atttaccagg cattcaatct gtttttcaat aagcggattt ctttttttgt
     2461 ttggcatttt atcctccatt gaggttctgg gttaaaaatg gagaccaaca cgctgtcacg
     2521 tgtggtcgtg cgccggacac ggataagaat ccggtactga cagtttactg aaaggatatt
     2581 tccctgaaaa gtcagtgcat aacgcgaaag cgtacggcga agctctttcc cttagaaggc
     2641 ttgtcgttag atttcttcga acgtgcgctt ccggttgtgg cactccgcga aatggcgcgg
     2701 cggtaagtat ggcggggtta ttccttcccc attgaggaca ccgggttgtc aggttgacca
     2761 tacgcttaag tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg
     2821 gcggtatcag ttttactccg tggctgctct gccgcccttt ttaaagtgaa ttttgtgatg
     2881 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga
     2941 ttctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg
     3001 catcacaaaa ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac
     3061 gtctgaaggt tgttttgata ttggtgttct gctcagtaac cgggagttta ctgaagatgc
     3121 cattaacatg aggaaatatg agccttatct gctcaatgat aattccatac tttcccgaat
     3181 tgctcttctt gaacttggta ttttcggaga acgtcaatga cttcagcatt tgcactgatg
     3241 atgacggttt ttcttataac gggtgaatca cagaatgtga ttaccggaat ttatgccagt
     3301 aaagaatcct gcctccaggc aagagacgag caaaaaattt ctggtgaatg cctcccggta
     3361 aaaaaagtat cgctgtacct gaataacgaa acaccggctg gataaccctc cagccatatt
     3421 aacaccatac caacggatta aaaatgccag caatggcagg gatttgttca cccttaaatc
     3481 tgtaatgagg tttatcaatg agcactgata aagaagaatt tgcgctatat tgcgaagcaa
     3541 aaaatgacaa agtcagaaaa cgtctgggaa ttaaaggtgg tttttactgg actacagcaa
     3601 aaaaattatc tgttgccatc tcccgctgca ttaccgcaat ggatgacaac gattatgatg
     3661 aagacgactt taaaaaaccc gttcgcgtcc atttacccgt tgtgaatgac cttccacctg
     3721 aaggcgtgtt tgataccgaa ttctgcaacc gatacgaaaa aggcggggaa gatggcatta
     3781 caatggtatt tatcgcgccc tctccctccg tgcaggagaa accagccagt actgacaata
     3841 ccaacgtcaa cggcgaagac atgacggaga ttgaggagaa tatgctcctg ccggtttctg
     3901 gtcaggagct gcccattcgc tggcttgcgc aacacggcag cgaaaaacca gtaacgcacg
     3961 ttgcacggga agaacttcag gcattacata tagcacgggc ggaagaactg ccggctgtta
     4021 ctgccctggc catttctcac aaaacaaagc tgctcgaccc gctggagatt cgcgaccttc
     4081 acaaactggt acgcgacact gacaaagttt tccctaatcc cggtaattca gacctgggac
     4141 tgataactgc ttttttcgag gcatacctgg acgctgacta cactgatcgg ggtctgctga
     4201 caaaagagtg gatgaaagga aatcgtgttt cgcgtatcac ccgtacggct tccggtgcaa
     4261 atgccggtgg cgggaacaaa accgatcgca atccgaattt agtacacacc ttcgatacgc
     4321 tggatgtgga gattgcagca gccacacttc cgatggattt taatatttat gaaattccgg
     4381 gcagcgttta tcgtcgcgca aaagaaatcg tcctgaaaag agaaagtccg ttcaaagaat
     4441 ggtccgcagc acttcgcgca accccgggta ttctggacta ttcccgcgcc gctatttttg
     4501 cacttatccg gagcgctcac cctgagtttt atcactaccc gggacgcctt caggggtata
     4561 tcaacgccta cttaacggag actgatcacg agaaccccag caaggaaact ctcactgctg
     4621 cacgacatac accggaaaaa gatatcctgg aagaagttaa ccgcgaactg tctgctaagc
     4681 aggaaacaga agaagaaaat gatgaagaaa aaccgcaacc atcttgcgca atggcagaat
     4741 gataatcaga agaaatctgg caaaagaccg ttttacagta ccaaaaacac aggcgggaac
     4801 caatcgtgtg attcacctta ttaagcccgc aatcgacgct ctccggagtc agatggcact
     4861 aacgagactg agcaaagagc atatcatcga tgttcacctc agagaattcg gcagaacaga
     4921 gaaacaaaaa tgtacctttg tttttcaacc tgaagtgtca gcgaaagtaa aaaattatgg
     4981 cgaccatttt accgttgact caataaggca gatgtgggac gcagcggtaa agcgtgccgg
     5041 aatccgccat cgcaaatcgt atcagtcgag acacacttat gcctgctggt cgttaacagc
     5101 aggagctaac ccggcattca ttgcaaacca gatgggccat gcagatgcgc aaatggtgtt
     5161 tcaggtgtac gggaaatgga tgtcagaaaa taataatgcg caggtaacgc tgttaaatac
     5221 acagttaagc gagtttgccc caaccatgcc ccataa
//
LOCUS       DAZGXS010000214         5030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGXS010000214 DAZGXS010000000
VERSION     DAZGXS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            221..1153
                     /gene="rfaD"
                     /locus_tag="ACHKG5_004574"
     CDS             221..1153
                     /gene="rfaD"
                     /locus_tag="ACHKG5_004574"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0870535.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1163..2209
                     /gene="rfaF"
                     /locus_tag="ACHKG5_004575"
     CDS             1163..2209
                     /gene="rfaF"
                     /locus_tag="ACHKG5_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0870536.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2213..3205
                     /gene="rfaC"
                     /locus_tag="ACHKG5_004576"
     CDS             2213..3205
                     /gene="rfaC"
                     /locus_tag="ACHKG5_004576"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0870537.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3202..4410
                     /gene="rfaL"
                     /locus_tag="ACHKG5_004577"
     CDS             3202..4410
                     /gene="rfaL"
                     /locus_tag="ACHKG5_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP0870538.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4446..>5030)
                     /locus_tag="ACHKG5_004578"
     CDS             complement(4446..>5030)
                     /locus_tag="ACHKG5_004578"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0870539.1"
                     /translation="LNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVI
                     GDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQE
                     PFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPE
                     LTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1355 a         1134 c         1171 g         1370 t
ORIGIN      
        1 ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca tgcaaaacca
       61 acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta gcatccttgc
      121 acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag acattcgtgt
      181 ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg ttaccggcgg
      241 cgcgggcttt atcggcagca acatcgttaa agccctgaat gataaaggca tcaccgatat
      301 tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg atctgaatat
      361 cgcagactat atggataagg aagacttcct gatccagatt atggctggcg aagagttcgg
      421 cgatgtcgaa gcgattttcc acgaaggcgc gtgctcttcc accaccgagt gggacggcaa
      481 gtatatgatg gataacaact atcaatactc caaggagctg ctgcactact gcctggagcg
      541 tgaaatcccg ttcctgtacg cttcttccgc agccacctac ggcggacgca cctccgactt
      601 tattgaatcc cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa aattcctgtt
      661 tgatgaatat gttcgccaaa tcctgccgga agcgaactcg cagattgttg gcttccgcta
      721 tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg tcgctttcca
      781 tctcaacacc cagcttaata acggtgaatc accgaagctg ttcgaaggta gcgagaactt
      841 taaacgcgac ttcgtctatg ttggcgatgt ggcagatgta aacctgtggt tcctggaaaa
      901 tggcgtttcc ggcatcttca acctcggcac cggtcgtgcg gaatccttcc aggctgtagc
      961 tgatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt tcccggataa
     1021 gctgaaaggc cgctaccagg cgttcactca ggcagatctg acaaatctgc gcgcggcggg
     1081 ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg cctggctgaa
     1141 tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt gggttggcga
     1201 catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc aggcgataat
     1261 cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg aagttaacga
     1321 agctattcct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg
     1381 tcatagtctg cgtgaaaagc gctacgaccg cgcctacgtc ttacctaact ccttcaaatc
     1441 cgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg gcgagatgcg
     1501 ctacggttta ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc taatggtgga
     1561 acgctatgtc gcgctggcct atgacaaagg cattatgcgc acagcacaag atctgccgca
     1621 gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca
     1681 attttcgctt tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg agtttggtcc
     1741 ggcaaaacgc tggccacact accactatgc ggagctggca aagcagctga ttgatgaagg
     1801 ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa gcaggcaatg agattcttgc
     1861 cgctttgaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa cacagcttga
     1921 tcaagcggtt atcctgattg cagcctgtaa agccattgtc actaacgatt ctggcctgat
     1981 gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccga gtagcccgga
     2041 cttcacaccg ccgctatccc ataaagcgcg cgtgatccgc ctgattaccg gctatcacaa
     2101 agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca ttactcccca
     2161 gcgcgtactg aaagaactca acgcgctatt gttacaagag gaagcctgac ggatgcgggt
     2221 tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg cactcactga
     2281 tgcccagcag gcaatcccag aaattaagtt tgactgggtg gtggaagaag ggttcgcaca
     2341 gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac gtcgctggcg
     2401 taaagcctgg ttctcggccc ctataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca
     2461 agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg cggcgctggt
     2521 tacgcgtctg gcacatggcg taaagcatgg catggactgg caaaccgctc gcgaaccttt
     2581 agccagcctg ttttacaatc gtaagcatca tattgcaaaa cagcagcacg ccgtagaacg
     2641 cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc agggcgatta
     2701 tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat atgccgtatt
     2761 tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc gagaattgat
     2821 tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc cgcatgagga
     2881 agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc cgaagatgag
     2941 tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg tggatacggg
     3001 gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg gaccaaccga
     3061 tccgggatta attggtgggt atggggagaa tcaagtagag tgtcgttcta caagtatgtc
     3121 tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa taataaccaa
     3181 taagttgaca tcggagataa gatgacctca acattatttt tctctctcga gaaaaaaaac
     3241 tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta ttttttgggt
     3301 gggattacaa ggtataaaca tcttattgtt attcttatga caataacgac aatcgtctat
     3361 ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa catttctttt tggtagtgtt
     3421 gccatattaa ctattgctgc attgctgtca cttcttcaat cccctgatgc aggtgctagc
     3481 atgaaggaaa ttttcaaagc tattattgag aatactttac tatgcacaat agcaataccg
     3541 atcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt ctcatttatt
     3601 agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga ctatcaacaa
     3661 gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat ggtcttttta
     3721 ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat ttcttttgtg
     3781 gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg ggcctggctt
     3841 tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa gttactatta
     3901 gtaggagtaa tggttgccat cattgcattg tcggttattt tcacacataa ggagatgacc
     3961 gcaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc aaatggtact
     4021 caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg ttacggtaac
     4081 gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg gacctttaga
     4141 caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg tttattaggg
     4201 ctggtaagtc ttatgatgct atactgtgca atattgaaag agtgtataaa aaatggcgtc
     4261 aagaataaat atcgctcacc atataatgca tattatataa tcttactatc ttttataggt
     4321 tattttgtta tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc
     4381 ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt
     4441 tttatttatt caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa
     4501 ctgtagtgat caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc
     4561 gctaacgttt ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg
     4621 aatcccgtag tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca
     4681 ggcttaccag cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa
     4741 ggaatgacga ctaaatctgc cagtgggtaa taacaatgca tcttttcagg cggtatgctt
     4801 cctaacataa tgcaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat
     4861 gcacctttgt cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt
     4921 ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg
     4981 acgatacgcc cagcataaaa aataattttc tcttcagggg agatattaag
//
LOCUS       DAZGXS010000215         4934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGXS010000215 DAZGXS010000000
VERSION     DAZGXS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            129..865
                     /locus_tag="ACHKG5_004579"
                     /pseudo
     CDS             129..865
                     /locus_tag="ACHKG5_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1044..1340
                     /locus_tag="ACHKG5_004580"
     CDS             1044..1340
                     /locus_tag="ACHKG5_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870540.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1648..1764
                     /locus_tag="ACHKG5_004581"
     CDS             1648..1764
                     /locus_tag="ACHKG5_004581"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870541.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1983..3359
                     /locus_tag="ACHKG5_004582"
     CDS             1983..3359
                     /locus_tag="ACHKG5_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0870542.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRRRIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPEELE
                     LFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEGKTL
                     CVAPNFDL"
     gene            4180..4743
                     /locus_tag="ACHKG5_004583"
     CDS             4180..4743
                     /locus_tag="ACHKG5_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0870543.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
BASE COUNT         1461 a          930 c         1116 g         1427 t
ORIGIN      
        1 tttcattttt gaatcaatat tttctgaaca gggtaatagt acacgtatta acagattatg
       61 tcagaaggcc ccggtgatgc cttctgacga tagattaatg ttgtattcta ttggtatctg
      121 gagaccatat ggcaacagtt acagtatatt ctccccgctg taattcctat aaagtgtacc
      181 gttatgatca cagcacctca gatcatgagc gtttccagtg ctaatcctgc aaacgtgtat
      241 ttcaactgaa ttacatctac gaaacccgaa aaccaggcat taaagagcaa attgttgaaa
      301 tggttcataa cggagccggg gttcgcgata gctccagaac gctgaaggtg gatatcaaca
      361 ccgtcattct cactttaaaa aacgctcacc acgtaaagta accatatctc cagccgccag
      421 tgtcgatgtg gcacttatct gtgaacttga tgaacaatgg agttttgtcg aaaacaaagc
      481 tcgtcagcag tggcactggt acgcgtataa gaccaaagct gacggtgtgc tggcttacac
      541 ttttggtcct cgcactgatg aaacatgccg tgagttgccg gaattcctga agccattctc
      601 cgcaggcatg ataaccagag acaaccggag cagttatacc agagaaatgc cacaggataa
      661 gcatcttgtc ggcaagatat ttacccggcg cattgagcgc aacaatctga cacttcgtac
      721 tcacatcaaa cgacctgccc ggaaaacgat ttgcttttta cgctccctgg aaattcacga
      781 aaagccattg gttcatttat cgaaaacaca tgtactactg acaggagtca ttaccagggc
      841 ctcttttgct gtttttttgc catgaatgat aatgagaatc gacagcgata agggaaaagt
      901 aaaataacac taatgctgtc gttttctatc atattgattt ttaattattt attgtctttt
      961 ttctcaaccc atttttttac gcagacaaat ttacccgggg aatacaaact gtaacgacag
     1021 ttcaccacac tatgggggat aaaatgccag atcaatccgg acactgcagg actccttcac
     1081 gcatcttacg gatgcctgaa ctgagccaat tgttaggtat atctcgttcg acaatttacg
     1141 aaaaaatgaa tccgttatcg aaatactacg atgcaacctt ccccaggccg gtacgtcttg
     1201 gtagtggttc tgtgggctgg cgttcatcag ctattgacga atggcttact ttacatactg
     1261 tacctgccag atcagcagtc aaaggtgtta atgacgaagt gagacatacg ctttcttcag
     1321 atatgaccat gagcagataa gtttttgtca tcacgtgatc acctcaggag gaaaaagaag
     1381 cataagaaca atttagccag aagtttaatt gataaacaga atttccagca ttcattgttg
     1441 aaagagcagc tattgtcgct attgtagcgg taattgctgc tataaccgaa acgtattacg
     1501 aaaaaggaag catttatgcc ttacatcaaa ccggatatgt cgtattcagt atatgcatga
     1561 ccatcaatga tagggaaggc aataattaaa gctcaataca ataaacaagt acctctgact
     1621 ctggttgcaa tgtcagtatt aacagcgatg tcaattgcct gtcagaatca ggttgacgta
     1681 tgcagtcccg gaaatttacg tggggccgtt aatatttaca ctatggttct ggagaaaggg
     1741 aaaagaccgg gggaaaggta ttagtgaaat aatttccgga ggtattaaga atgagaaata
     1801 tgaatgatat ttcgtggtga ataatacagt cttcactgaa ttaaccttat cggtttatta
     1861 aacatcagat ctacaaatgc tctgtatttc tcagagcatc aggtctgaat agcagctatt
     1921 ttcgctattg tagcgggatt tgctgctatt ttactaaccc attaatgaaa agagaggctt
     1981 ttatgtgctt acttgcacca gaaaatcctt atccgatata tgcactacca ccgctggtga
     2041 gaaatgcaat aattgaaact caaaaaaata cacaggcacc tttggctatg gtggcaacat
     2101 ccgcattaac agcgatctca attgcctgtc agaatcagat tgacgtgtgc agacctggaa
     2161 atttacatgg gcctgttaat ctttactctc tgattctggc tgattccggt gaaaggaaaa
     2221 cgaccgtgga taaagtgttt atgaaagcat tttatctcag ggatgaagcc ctggcggatg
     2281 aatacgcgaa actggttgag aattacagta cagaaaagga aatatgggag caaaaacaaa
     2341 aagcgctgga atcaaaattt cataaagaga ttcgtgccgg taaagattac aaggcaacag
     2401 aatcagagct tgaaacgcat ctgaataaac ctcctgttcc gccgcagata cgtcgaacaa
     2461 tttttaatga gacaacgata gagggaatgt taaaatatta ctccgatagc aatcgctctt
     2521 ttgctcttgt atccagtgaa gggggggtaa tttttgacag ccgggccatg agtaaactgg
     2581 gaattattaa cactttgtgg gatggaggtt ctcttttcat cgacaggaaa tcatctcccg
     2641 gaattaattt gaaggagcca agactgacga tatcggtgat gattcagcct gatgtttacc
     2701 acaaaggttt ttgtacgcga aaaaaagaaa ttgtgaaaac gtcaggacat catgcaaggt
     2761 ttttgatgtg tcaaccaaca tcaacgcagg ggacaaggag gaggatcaat gagcttattg
     2821 atgaaagtct ggcgatgagt ggtgaacgac gttgccttca cttttctcct caggctgcca
     2881 gaatctggac ggattattac aatgatgtgg aatcgaagct ggggggattg gggcctttaa
     2941 gacattgccg ggaatatgct gctaaaaatg cagagtatat ggcaagactt atttaccatt
     3001 cgagcggtga agagggggag atttcccctt acactgcaga aatggcgaga gaattagcaa
     3061 tatggtacgg taatgagtat gtgcggttgt ctaatccgtt aacttttgac aactctgccc
     3121 tgaccgtacc tgtgcgactc attccagagg agcttgaact tttcaactgg ataaaaagct
     3181 attgtattga gaaggggatc ctctgtatga aaaaaaatga cattttacag cgtggtccga
     3241 accgtttccg gaaaaaggat aaaatcaact ggttactgga tttactgtat gaacaaaaca
     3301 gagttgtacc ggttattgag ggaaaaacgt tgtgtgtcgc acctaacttt gacctctgaa
     3361 cgacttaatc gttgagtaca tatagtctcc tctgctactt gcggaggaga ctaatgtctt
     3421 taccatgtca gcgttgtatt agaaaactct ggtacaggtc agtcagtaaa tataactggt
     3481 gtaaccacta tttactattg gatagagtgt tgctcaggat attgtcggct ttagtctgag
     3541 gtgaggtggg gccttcattg ttttcgtgtt caataaataa tttaggttaa ttagtattat
     3601 tagtggaagt tatgtgatct gttatgttgt cttgtacgca cttaacactg gttacgtacc
     3661 ggaacactat tagagtatgt tatggctctg ttacagttta taggtatgaa aaaatataat
     3721 ctttcctgta tgaaataata ttcttatggt gtgtggggta tggtctgtat accatcattt
     3781 ttatggtgaa gttaaatatc tgtagaggcc ttaagctggg tcgctgtttt tacttaatgg
     3841 tacagaagta ttaatagcgg tgttgcctgt ggctacccga tactggcttt tgtgaatgtc
     3901 agattacttc ctgtaagtga ttgaatggta cttccttaaa ctcaattcta tgaggtgtgt
     3961 ttttaccatt gtaattcgta ctggtcctca gtttaagtga gtcttactgt actctgaaat
     4021 attacctctt tggagtaatg aggtaatacg aaacagtttt atcaggttgt ctgtgctgct
     4081 ggttacctcg tttattaatg ccgcggtacg gcgtttctgt actctcagta tttatgtaat
     4141 tccaccttcc tgtaactttt ataaagtaag gactataaaa tgtataatgc taatcccaat
     4201 tacgaaatgg acttcatgat tctgaaagac gtgaatgaac acatggaagg tctgtttcaa
     4261 cgtttttcta aactgttacc tttcagaatt gattttgcct acaggaaaga cacgcccagt
     4321 tttggtcatt cctgtaggca cagtatgtgt attgaaatgc accgcttact tagtgaaacc
     4381 caaacgatgc tggcgggata ttactgggta atggaatata cgtcaaacaa gggacttcat
     4441 attcatttta tcggctacct ggatgggcaa cgtcacaaca aatcttatcg gatatccagg
     4501 caactgggag atatctggag gcggatcacg gaaggggagg gttattttca cctgtgcagg
     4561 gctaaagaca aatacccggt gcgtatcgat cacgtaattc actatagtga taaatctgcg
     4621 gtagacgact tacgttacgc ccttagctat ctggccaaac aggatcagaa agaacacggt
     4681 attattctga ggcgtagcag gcttcccgaa aaaagtaacc ggggccgacc acgacataac
     4741 tgaattacag ctgtattagg tattagaatt aagtttctgt aagttgttgg gaggtgcttg
     4801 agttaatagg cagaagaggg cggggcgctt tgtcatcagt gtaggggacc cgaatttctg
     4861 ccgtggtgcc tcagtggatc ggatacaggt aacgaatatg gttcataaat ctgacagtga
     4921 tgaattagct gcat
//
LOCUS       DAZGXS010000216         4826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGXS010000216 DAZGXS010000000
VERSION     DAZGXS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            123..2810
                     /locus_tag="ACHKG5_004584"
                     /pseudo
     CDS             123..2810
                     /locus_tag="ACHKG5_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2926..3990
                     /gene="hipA"
                     /locus_tag="ACHKG5_004585"
                     /pseudo
     CDS             2926..3990
                     /gene="hipA"
                     /locus_tag="ACHKG5_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            4261..4530
                     /locus_tag="ACHKG5_004586"
                     /pseudo
     CDS             4261..4530
                     /locus_tag="ACHKG5_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1299 a         1031 c         1305 g         1191 t
ORIGIN      
        1 tgacactttc ccacctccaa ctaacactca atacagacct ctgacaattt aataaatttc
       61 atttttaatg tctgacgact catctctttc gtggctgtat tgtttgtatt tggagcgttt
      121 atatgaatag aatctatcgc gtgatatgga attgcactct acaggtattt caggcctgct
      181 cggaattaac tcgcagggta ggtaaaacat caacggttaa tttgcgaaag tcctctggac
      241 tgacaacgaa attcagtaga ttgacgctgg gtgttttgct ggcactaagc ggttcagtgt
      301 ctggtgcgag tctggaagtt gataatggtc agattaccaa tattgatact gatgttgctt
      361 atgatgccta cctggttggc tggtatggca ctggagtgct taatattttg gctggcggta
      421 atgcctcctt aaccactatt actaccagcg tcattggcgg taatgaggat tcggagggta
      481 ccgttaatgt tttgggtggc acctggcgat tgtatgatag cggaaataat gcaaggcctt
      541 taaatgtggg tcaatccgga acggggacgc tgaatattaa acagaagggt cacgtcgatg
      601 gaggctattt aagattaggt actcaagctg caggcgtcgg gacggttaat gttgagggag
      661 aggactcggt tttgacgacc gaattattcg aaataaaggt gggatcagtc agggtgaact
      721 gacaggtggc ggaaacctga atgttacagg gggaacgctg gctgtcgagg ggcttaatgc
      781 acgctacaat gcgttaacca gcgttagccc aaatgcggaa gtcagcctcg ataatacgca
      841 ggggttaggc agaggaaata ttgccaatga cggtctgtta acgctaaaaa acgtgactgg
      901 cgaactgcgt aatagcataa gcgggaaggg tatcgtgagc gcaaccgcca ggacagatgt
      961 agagctggat ggcgataata gccgctttgt ggggcaattc aacattgata caggcagcgc
     1021 gctcagcgtc aacgagcaga aaaacctggg tgatgcttcc gttatcaata atggcctgct
     1081 caccatctcc actgagcgta gctgggcgat gacgcacagt atcagcggta gcggtgattt
     1141 gacaaaactg ggtaccggga tcctgactct taacaacgat tcctcggcgt atcagggtac
     1201 gacggatatc gtggggggga aattgctttc ggttccgact ctgccattaa tacggcaagt
     1261 caacacatta atatccataa cagcggtgtg atgtcgggaa atgtcaccac tgcaggtgat
     1321 gtgaacgtta tgtctggggg gacactgcgt gtcgctaaaa ccacaatcgg cgaatcggcg
     1381 gcaacctgga gaatggcggc acggttcaaa tgaacagtga aggaggaaaa ccggggaatg
     1441 tactgaccgt taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc
     1501 tgggcggcga taattcaccc accgataaga tgaacgtgaa aggcgatacc caagggaaca
     1561 ctcgcgttcg ggttgataac attggcggcg tcggtgcgca aacggtcaac ggtattgaac
     1621 tcattgaggt tggcggtaat tctgcaggta atttcgcgct gaccaccgga actgtcgaag
     1681 ctggggctta cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc
     1741 tgaccagtaa atgggacggc gtaacgccag cggatacacc cgatcccatc aataatcccc
     1801 ctgttgtgga tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca
     1861 acattgccgc agccaactcg ctgtttagcc atcgcttaca cgaccgtctg ggtgagccgc
     1921 aatatacaga ttcactgcat tctcaggatt cagcaagcag tatgtggatg cgtcatgtcg
     1981 gggggcacga acgttccagt gccggagacg gccagctaaa tactcaggct aaccgctatg
     2041 tattgcagct aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatc
     2101 ttggcgtgat ggcaggctac gccaatcagc acagtaatac tcagagtaat cgtgtgggtt
     2161 ataaatcgga tgggcgcatc agcggttaca gcgctgggct gtacgcgacc tggtatcaga
     2221 acgatgcgaa taagaccggc gcttatgttg acagctgggc gctgtataac tggtttgata
     2281 acagcgtcag ttccgataac cgttctgctg acgactatga ttctcgcggt gtgagggcct
     2341 ctgttgaggg tgggtatacc tttgaagcgg gaacatgtag cggcagcgaa gggacgctga
     2401 atacctggta cgtccagcca caggcgcaaa tcacctggat gggtgtgaaa gattctgacc
     2461 atgcccggaa agacggaacg cgcattgaaa cggaaggcga cggaaacgtg caaacgcgac
     2521 ttggggtgaa aacctacctg aatagccatc accagcgtga cgatggtaaa cagcgtgagt
     2581 tccagcctta cattgaagcg aactggatca acaatagcaa agtctacgcc gtgaagatga
     2641 atggtcaaac cgtaagccgt gatggtgcgc gaaatctcgg tgaagtacgt accggggttg
     2701 aggcgaaagt aaataacaac cttagcctgt gggggaatgt cggtgtgcaa ctaggtgata
     2761 aaggctatag cgatactcag ggcatgctgg gagtgaaata tagctggtaa acagtataag
     2821 ccgcatgtcg agatggcatg cggcttaata ttgccaactt caaaaggctt ttaaagcctc
     2881 ccttttatcc gcgatcgcgg atatcgcagc gtttatcccg tacagtttga tttattgtca
     2941 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg
     3001 cgtccgataa tggcatggga aaagcttact gaagtcagac ttgaagaagt attaacggct
     3061 tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt
     3121 gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa
     3181 ggaataacgc cgacgacgca catcattaaa ttaccgattg gggaaatcag gcagcccaat
     3241 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     3301 gaacttgggt tgaatgtccc ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta
     3361 gcggtcgaac gttttgacag gcgttggaat gctgagcgaa cggttttact tcgcttgcca
     3421 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     3481 ggcccaggca tcgcgcggat catggctttt ttgatggggt tcagcgaggc gcttcgcgat
     3541 cgttatgatt ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac ggatggccat
     3601 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     3661 gacatcattt cagcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa
     3721 ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg
     3781 cgacattttt tggcgacagc aaaggtgctg agattcccgg aagtgcagat gcatgaaatc
     3841 ctgagtgact ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg
     3901 acagattttc cagagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcacggt
     3961 cggttaagcc gagaatacgg tattaagtaa gagtaagata tggggtttgg tcgttgttaa
     4021 tgagcaggac aatcatgacc gccaacgact agattctccc ttggtttata ttggtgcgca
     4081 taatgtatat tatgttaaat cgtatctgag tgatacgaag tgattttccg gtagtcgtta
     4141 ttcgttcagg attccagaac ttaagcttct cctctttcat aattggcctg tgcatgacgg
     4201 atataggctg gctggtatgc cgatttgccg tttgccgcat cttcccagcg ttgaagactc
     4261 aagctggcaa accgcgtgca aatgagcacg ccctctttcc atcaacactt caggcagctt
     4321 gcgggaatga gtccactccg gtatcagaaa tggctacgtc tcaatgaagt caggcgaacg
     4381 atgctgaatg aacattacga tgtcaccaca gcagcctatg ctgtcggtta cgaaagctta
     4441 tcccatttcc gacgggaata tttgcggatg tttggagagt cacctaagag agatattacc
     4501 aggttaagaa agtctgtcgg tcagctttaa taaaccctgc acttatctgt ttagagcaat
     4561 tcggtgttag ttacaccagg caaacattaa ctatagctaa tgatttatag ccatataaac
     4621 cattagtagc ccttgacctt tcctgacact tcccttgttc gcaataatat tctgtttagt
     4681 cacggtttat aaataacatg cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt
     4741 tttgcaaata ctctcttcaa caagtgcagc aataaacttg ttgtgattag gtgtccttgt
     4801 gatttaaaga tattatcgtt cttcgt
//
LOCUS       DAZGXS010000217         4782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGXS010000217 DAZGXS010000000
VERSION     DAZGXS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            161..727
                     /gene="slp"
                     /locus_tag="ACHKG5_004587"
     CDS             161..727
                     /gene="slp"
                     /locus_tag="ACHKG5_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0870544.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            883..1413
                     /gene="dctR"
                     /locus_tag="ACHKG5_004588"
     CDS             883..1413
                     /gene="dctR"
                     /locus_tag="ACHKG5_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0870545.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1474..2502)
                     /gene="chuS"
                     /locus_tag="ACHKG5_004589"
     CDS             complement(1474..2502)
                     /gene="chuS"
                     /locus_tag="ACHKG5_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP0870546.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2551..4533)
                     /gene="chuA"
                     /locus_tag="ACHKG5_004590"
                     /gene_synonym="shuA"
     CDS             complement(2551..4533)
                     /gene="chuA"
                     /locus_tag="ACHKG5_004590"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP0870547.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1277 a         1169 c         1080 g         1256 t
ORIGIN      
        1 tactcatgcc acgccccaaa ataacggaat tgaactaaac agaagggata taaacatcag
       61 acaggtttac gttactatca ggcatatcgc ctcagaatca gatgaaaact ataaagaaat
      121 atctattatg gttttaatat ttgttgataa ggatagtaac atgaacatga caaaaggtgc
      181 actcatactc agcctttcat ttttgcttgc agcatgtagt tcaattccgc aaaatatcaa
      241 aggaaataac caacctgata ttcaaaaaag ttttgttgct gttcataacc agccggggtt
      301 atatgttggt caacaagcgc gctttggtgg gaaggttatc aacgttatca atggcaaaac
      361 ggatacgttg ttagaaatcg ctgtattacc gttggatagc tatgcgaagc ctgatattga
      421 agccaactat cagggccgac tgctcgccag acaaagcggc ttccttgatc cagtgaacta
      481 tcgtaatcac tttgttacca tcctcggcac cattcagggt gaacaacctg gctttatcaa
      541 taaagtcccg tataacttcc tggaagtgaa tatgcagggc atccaggtgt ggcatttgag
      601 agaagtggtt aataccacct ataacctgtg ggattacggc tatggtgcat tctggccgga
      661 accgggctgg ggtgcgcctt actacaccaa tgcggtgagt caggtaacac ctgagctggt
      721 caaataacac caccgaaaga tgcagatgct ttctcagcat ctgcatcatg cattacatca
      781 aattaataca cagtaagcta actattatta ttataagccc tgtcctgtta attacctttg
      841 gcaaactgat tataaagtta atgtccgcac caggagtcgg ttatgtttct tataattacc
      901 agggatacga tgttcttcac cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat
      961 atacagaacg aagaagagat cgacgtaatg ttgcatcaga atgccttcgt cattattgat
     1021 acattaatga ataatgtatt tcattctaat tttctcactc aaattgaacg attaaaacct
     1081 gtccatgtca ttattttctc cccctttaat attaaacgct gcctggggaa agtgccggtg
     1141 acctttgttc cgcggactat cactatcatt gattttgtcg cactcatcaa tggcagttac
     1201 tgctctgtgc ctgaagcgaa tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc
     1261 attgcgaatc aaatgacaac ggaagatatt ctggagaaac tgaaaatatc gctaaaaacg
     1321 ttctactgcc ataaacacaa tatcatgatg atcctcaatc ttaagcggat caatgagctg
     1381 gtacgccatc agcatattga ttatctggtg tgaacgattg atcaatataa agaggcccag
     1441 caataaatag ccagacctcc cgttaattat acgttatgca gcaacgcctt ccggtatcag
     1501 cgaagcaatt tgcttacgcc attgcgcttg ctcctgttcg ccttctgtac gttgaccata
     1561 aagttgcgct atctgcgtac catcatgggc aaacaattcc agactggtta cgtagccatc
     1621 gctggtcggt ttacgggtaa cccaggcttc agcaatgctc tcttctaata gatgaagagt
     1681 aaacgtcggg ttgaaaatat tcagccaacc tttcattggc accacttttt ctaccacacc
     1741 ggtgaaaatc tgtacgcagc cacggttgcc aacaaacacc atgatttcat taccatcctg
     1801 ctgtgcagat tcaagaattt gcgccaacgc actgttggat actttgcagg ccaaatcgtc
     1861 tgccaccaga ttgaacgcct gttggcgcgt caggttgtgg cgcttgagca acgtaaaaaa
     1921 ctgatgaacg tcggtcatcg cccgccactc ttgctcgacc acagtggcat cggctcgcgt
     1981 ttgaacaact ggcgcatcaa cggcttttaa ctcaagcggc gtattctcat cggtgataaa
     2041 ccgtgccaga agctcactcc atgccgccat atcggtattg tcggtggcat aaacttttag
     2101 taatgcatcg ccctgatgat caaagaactg aatactctgg cgttcgccac gagccgtgtt
     2161 ttctttgatg tggaaaacac tggcccattg attgagaaac agacgtaaat ccagcgcgcg
     2221 cggattgagg atcaatccgg catgtccgtt caaatgctgg tttgtgaacg taccaacttg
     2281 ctcatggact gcatattcat tacgacaaat acatttggtt tcgccaacac tttcgagcgc
     2341 cgccagaatt tcacggatat cgccgtgcat ccgccacgca tcgtgcgtga cgcgtgcaaa
     2401 tgccagttct gcttctctaa tattcattaa ccctgcgatg tcacgcgcgt actttccggg
     2461 attttgttct tttaactcaa gccagcgtgt gtagtggttc attgtctctc ttccttccag
     2521 ataaatgccc cgaaatatcg gggcagatga ttaccattga taactcacga aaatttttcc
     2581 gttacgacca tcctgtggga tgccttgcgg cgaccagtac tctttgtcga aagcgttacc
     2641 caacaccaaa gtagtggtca tacctttgag cgcctgttgt ccttgataac tgacgtagaa
     2701 atcattcacg ccatagcctg gttgtttgct gtaactgctg ctgatatgtg ttgagcgatc
     2761 ggcaaacgta ccaacccacc caacagagaa gccactgtga gcgatcggaa tattcagagt
     2821 gctggtaaca gtatccgggt taatgctgga gatgtattcg ccggtatcgg tgtctttgcc
     2881 gcgggtacgg ttataggcca catcaaggct aaacagatca gtggtatatt tcgtcatcac
     2941 atcccagccc cagattttgg cgttcgggac gttatacgac atagtcgtcg ccgccgcgaa
     3001 atcgacggtc gtggagatgt aatccttcgc tttggtatca aagtagctgg ctttaaattc
     3061 cagagcatca ttggacaaca tcaggtcatc aaaacgcagc ccaaaaccgt actcctgagt
     3121 ttcgttagtt tccggacgta agttcgggtt tggcacccaa tagttggtat agaagcgacc
     3181 aatcgagaag tgcttagaat cgttatacat ttcgcccatc gtcggggcgc ggaatgcctg
     3241 ggcatatgag ccaaataaca tcagccagtt agtcggattg atagtcatcc ccgcacgaga
     3301 tgaccatttg tcggcatcaa catctttgta accgtcactg ctaccgcgat aactgtcata
     3361 gcgggttccg ccaagcaggg taatcggcag atcgcgtaag gtgatctcat cctgtagcca
     3421 gccggagcta aaatcgattt ttgcttgcgg gaagcccgtc gtcgcgccgc ccggatgttg
     3481 ttcctgacga taatactcac cgccatatgt cagtaagtga gaagcgaaac tgtcggcaaa
     3541 gagagtggaa cggttctcca gcctggctcc ttttgttatc tgttcacgat actcgccgga
     3601 actccccgtg ttttgcgcat taatacggac ttccgaccaa taaatttttg catctgcatt
     3661 taaccagtcg ttgccctgcg gggcgagttt ataagaaagc tgcgcatcgc gttgaattgt
     3721 tgaacgatca accatcgggt tgctgctttc agaagcctca acggtctgcg gattttttgg
     3781 ttcacgcgcg tcgttgttgt agtaacgcac taaaccgctc agagactggg ctgaatcaat
     3841 ttgccaggtc cctttcgcca gcatgttatt aatggactcg tcattcggcg cggtttcacc
     3901 attgctctgg cgtaaatcac cccgatcgcg actggaccag gccacaatac catccagatt
     3961 ttcagttcgc ccaaacgcgc tcgcgcctaa tcccaggcta tggtcccccg tgccgccagt
     4021 accaaagaca cgaaaaccac tgctttgtcc ttcctgcaat aaatcttttg catcgaccgt
     4081 atcgtaggag atcactccac ccagcgcgcc actgccatac agtaatgctg aaggtccacg
     4141 aacaatctca acacgcttga tcagcgccgg atcgagaaaa gtgccattca ggtgtccggt
     4201 atccgttccc tgacgaacac catcgacaag aaccagcacg ccgcgatgat catagccacg
     4261 catatttaca tcctgaccgt tggttcgtcc ggtaccatcc agagtaattc caggaacatg
     4321 acgcagcaga tcggtggctg aagtagccgt ttgattttca ggagcggaag tgtcgatgac
     4381 gctgaccatc ataggcgctt cgaaggaact acgggcattc cccgttgccg taacggtcat
     4441 ggtttcagta gcaaaagcaa acgttggcaa ggtggcagaa acagctaagg ccaataaact
     4501 caaacgcaac gaggtaaatt gcggacgtga catagcgatt ctccatgagg aaaataaaaa
     4561 atgctggctg cctgggttaa taccggggtg gctggcgaaa aataaattta tttaaaattc
     4621 agagcgataa tattcgccgg gtgagtttac aagcgtttat atcccatgaa attgcctgat
     4681 aactcggcaa ttacgcaaaa gcacgccctc tttgtcaggc aaaacgatat gccccatcag
     4741 gagtgaagag agctgtgcgt tatcccttgc aaatgctatg gt
//
LOCUS       DAZGXS010000218         4768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGXS010000218 DAZGXS010000000
VERSION     DAZGXS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            131..673
                     /locus_tag="ACHKG5_004591"
     CDS             131..673
                     /locus_tag="ACHKG5_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0870548.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            773..1975
                     /locus_tag="ACHKG5_004592"
     CDS             773..1975
                     /locus_tag="ACHKG5_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0870549.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2195..2977
                     /locus_tag="ACHKG5_004593"
     CDS             2195..2977
                     /locus_tag="ACHKG5_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP0870550.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2992..4197
                     /gene="rhmD"
                     /locus_tag="ACHKG5_004594"
     CDS             2992..4197
                     /gene="rhmD"
                     /locus_tag="ACHKG5_004594"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFP0870551.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4254..>4768
                     /locus_tag="ACHKG5_004595"
     CDS             4254..>4768
                     /locus_tag="ACHKG5_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0870552.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMG"
BASE COUNT         1096 a         1170 c         1302 g         1200 t
ORIGIN      
        1 aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc gctcattgaa
       61 tcctctgcgc caggcagtac aatcccgcag ctttgggaat tttgattaat tgcttgtggg
      121 aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt tggtttctgc
      181 atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca atattggtgt
      241 cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac ataacgacga
      301 cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc cgttaatggc
      361 gaccgttggc ggaaaaggcg tttacaccaa cccgaattac ggcgatgaag gctatgccgc
      421 agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt ttggcgagta
      481 ttactactct ccggattcac tctccagcgg tattaaaagt tatgaggaag cgaatgctgg
      541 cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct acctgaatct
      601 gtcgggtaaa gacggtaacc gcgacaacgc cgtggctgac ggcccgtatg ttggggttaa
      661 cgccagtttc tgatcccttc cggcgcggca ttctgtcgcg ccattcagtt tcttactacg
      721 ccttatctcc ctttgcgtta tagtgattcg ataacaaaag caggagataa ccatgttaaa
      781 agtggaaatg ctatccaccg gggatgaagt gttacacggg caaatcgttg acactaacgc
      841 tgcctggctg gccgattttt tctttcatca ggggttgcca ttatctcgcc gcaatacggt
      901 gggggataac cttgatgact tagtcaccat tcttcgcgaa cgtagtcagc acgccgatgt
      961 gctgatcgtt aacggcgggc tgggaccgac cagcgatgat ttaagcgcac tcgccgctgc
     1021 gacagcaaaa ggtgaaggcc tggtgctgca tgaagcctgg ctcaaagaga tggaacgcta
     1081 tttccacgaa cgtggacgag taatggcacc gagcaaccgt aaacaagcgg agctgcctgc
     1141 cagtgctgaa tttatcaata acccggtagg caccgcctgt ggttttgccg tgcagcttaa
     1201 tcgttgcctg atgttcttta ctcccggcgt accgtcagaa tttaaggtga tggtcgagca
     1261 cgaaatcctg ccgcgtctgc gcgagcgttt ttctttacca cagccgccgg tttgtctgcg
     1321 tttaactacc tttggtcgtt cggaaagcga tctggcacaa agcctggaca ctctacaact
     1381 gccgccgggc gtaacaatgg gctatcgctc ctcaatgccg atcatcgaac tgaaactcac
     1441 cggaccggca agcgagcaac aggcgatgga aaaactgtgg ctggacgtta aacgtgttgc
     1501 cggacagagc gtgattttcg aaggcaccga aggactgccc gcgcagatca gtcgcgaatt
     1561 gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc accggtggtt tattggcttt
     1621 gcaactttct cgcgcaggtg ctccactgct ggcgtgtgaa gtggttcctt cacaggaaga
     1681 aaccctggcg caaactgcgc actggattac agaacggcga gccaaccatt ttgccgggct
     1741 ggcactggct gtttcgggtt tcgagaacga gcatctcaac tttgcgctag ccacgccaga
     1801 tggcacttta gctctgcgtg tgcgtttcag cactacacgc tatagcctgg ctatccgtca
     1861 ggaagtgtgc gcaatgatgg cactgaatat gctacgccgt tggttaaacg gccaggacat
     1921 tgccagtgag catggctgga ttgaggttgt tgagtccatg accttatccg tctgattccg
     1981 ccactctgtg ccggtttact gcaccggcac attccccctc cggcaaaact tgtccaacct
     2041 gttgtactca ataagaaaaa atccgtgcgc tgattcacaa atttgtgcgt aatttctcca
     2101 gtccgttgga cgaggagcgg gatttgatgc gcttcacaat tttactcccc ctgagcaacg
     2161 agactgattc cagtatctaa gactggagtc cagtatgttg gaatcaagca aagttcccgc
     2221 cctgacacgg gctattgata ttctcaattt gattgcccgc attggcccct gtaatgctgc
     2281 gacgatcatt gacacattgg ggatccctaa aagcacggcc tatttgctgc ttaatgaact
     2341 caggcgtcag cgttttctta gccttgatca ccaggaaaat ttttgtttgt ggaccaagct
     2401 ggtggagctt tccggtcatg cgttaagcaa gatggatctc cgggaactgg ctcgaccgcg
     2461 tttgacgcaa ctgatggata ccaccgggct gttatgtcac ctggggatta ttgataacgg
     2521 cagcgcttat tacattttga aagtggagtc atcagccact atcagcgtgc gttcacatga
     2581 aggaaaaagc ctttcgcttt atcgctccgg aataggtaaa tgcctgcttg cctggcaacc
     2641 tgcaactgta cagcaaagta ttatcgaagg gctggtctgg gagcaggcga cacccacaac
     2701 gattactcat ccacagcaac ttcacgaaga actggcgcgt attcgccgtc agggctggag
     2761 ctatgacaac ggcgaagatt acgccgatgt ccgttgtgtc gctgcgccgg tgtttaacgc
     2821 caataacgag cttaccgctg cgatctcggt ggtcggtacc cgcttacaaa tcaatgaaga
     2881 ataccgtgat tatctggcgg gcaaggccat tgcctgcgcc agggatattt cacgtctgtt
     2941 gggatggaaa agtccctttg acttacaagc ctcataacaa tggagaacat catgacccta
     3001 ccaaaaatta aacaggttcg cgcctggttt actggcggtg cgacagcaga aaaaggcgct
     3061 ggcggcggtg attatcacga ccagggggcg aatcactgga tagacgatca tattgccacc
     3121 ccgatgagta aataccgcga ttacgagcag ttacgccagt catttggcat taacgttctt
     3181 ggcacgttgg ttgttgaagt cgaagcagaa aacggccaga ccggattcgc tgtttcgaca
     3241 gccggtgaaa tgggctgttt tatcgtcgaa aaacacctta accgtttcat tgagggtaaa
     3301 tgtgtcagtg atatcaaact gatccatgat caaatgctca atgccaccct gtattactcc
     3361 ggctctggtg gcctggtgat gaacacgatt tcctgtgtcg atctggctct gtgggatctg
     3421 ttcggcaaag tggtcgggct tccggtttat aaacttttag gcggcgctgt tcgtgatgag
     3481 attcagttct acgccacagg tgcgcgtccg gatctggcaa aagagatggg ctttatcggt
     3541 ggcaaaatgc cgacgcactg ggggccacat gatggcgatg cggggatccg caaagatgcc
     3601 gctatggtcg cggatatgcg tgaaaaatgc ggtgaggatt tctggttaat gctcgactgc
     3661 tggatgagtc aggacgtgaa ctatgcgacc aaactggccc acgcttgcgc gccctataac
     3721 ctgaaatgga tcgaagagtg cctgccgcca cagcagtatg aaggttatcg cgaactgaaa
     3781 cgcaacgcgc cagtcggaat gatggtcacc agcggtgagc accacggcac actgcaatct
     3841 tttcgtacgc tttcagaaac cggtatcgac attatgcagc cggatgttgg ctggtgcggt
     3901 ggcttaacca cgctggtgga aattgccgca atcgccaaat ccagggggca actggtggtg
     3961 ccgcacggtt cgtctgttta ctcccaccat gcggtgatca ccttcactaa tacgccattc
     4021 agcgaattcc tgatgactag tccggattgt tcaacgatgc gtccgcagtt tgacccgatt
     4081 ctgctcaatg agccggttcc ggtgaatggt cgtattcata aatcagtgct tgataaaccc
     4141 ggttttggcg tcgaactcaa tcgtgactgc aatttgaaac gcccctacag ccactaatca
     4201 ctccgggcgt tgctgtgaca acggtaacgc ccacatattc taaggagctt tttatgagca
     4261 ccgctttgct tgacgccgtg gtgaagaaaa accgtgtgcg tttaattccg tttatgttgg
     4321 cgctgtatgt gctggcgttt ctcgaccgtt cgaatatcgg ttttgccaaa cagacctacc
     4381 agattgatac cgggttgagt aatgaagctt atgcgctggg agcaggcatt ttctttgtgg
     4441 tatatgcgtt tctgggtgtt ccggcgaatc ttttgatgcg caaactgggg gccagaacct
     4501 ggattggtac gacaacactg ctgtggggat ttctttcggc agccatggca tgggccgata
     4561 ctgaagcgaa atttctgata gttcgcactc tgcttggtgc tgcggaggcc ggatttttcc
     4621 ctggtatgat ttatctcacc tcgcaatggt ttccgcagcg taatcgcgcc agcattatgg
     4681 ggctgttcta tatgggcgca ccgctggcgt taacactggg atcaccgctt tccggcgcgc
     4741 tgctggagat gcatggattt atggggca
//
LOCUS       DAZGXS010000219         4566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGXS010000219 DAZGXS010000000
VERSION     DAZGXS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            144..569
                     /locus_tag="ACHKG5_004596"
     CDS             144..569
                     /locus_tag="ACHKG5_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0870553.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            566..640
                     /locus_tag="ACHKG5_004597"
                     /pseudo
     CDS             566..640
                     /locus_tag="ACHKG5_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(869..1051)
                     /locus_tag="ACHKG5_004598"
     CDS             complement(869..1051)
                     /locus_tag="ACHKG5_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870554.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            1379..2251
                     /locus_tag="ACHKG5_004599"
     CDS             1379..2251
                     /locus_tag="ACHKG5_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0870555.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2623..>4566
                     /locus_tag="ACHKG5_004600"
     CDS             2623..>4566
                     /locus_tag="ACHKG5_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFP0870556.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVN"
BASE COUNT         1153 a         1143 c         1218 g         1052 t
ORIGIN      
        1 acggtgtatt acctccgctt tgtaaagacc attgatgctc tccgccatcg cgttgtcagt
       61 aagcgtaaac tgaccgccgt atgtagccat cagacgaaaa ttggtaactt agacgcccat
      121 ctgatataga cggacatcta agtatggaat tacaggactg gcgaaaagaa cctcgtaaaa
      181 actattcgaa tgaattcaaa cttcgtatgg tggaactggc atcacaacca ggagcctctg
      241 ttgcccgtat tgcccgggaa cacgatatca atgataacct cctgttcaaa tggctcaggc
      301 tctggcagaa cgaagggcgt atatcacggc gtcttcaggt aacgacctct tctgacactg
      361 gcgttgaatt attacctgta gaaataacgc cggatgagca gaaagaaccg gtggcggcca
      421 ttgcgccgtc tttatccact tccactcaga ccagtgtcag tgccggctcc tgcaaggtgg
      481 agttccgtca cggtaacatg acgctggaaa atccatcgcc agagctgctc acgctgttga
      541 tccgtgaact gaccgggagg ggacgatgat ctcactcccg tcaggtaccc gcatctggct
      601 cgttgccgac gttaccgata tgcgtaaatc cttcaacgga tacgacatat gctgaaggag
      661 gagcagtaat ggattaagtg ataacaaata cccagaaata gaggttcaaa tccagcttag
      721 ttagtacata aatactattt tgatgaaaat tattatatgt ttcctcagat tttaatcagg
      781 cgcccagatt aaaccaagta ttgagcgcct gattatgttt attcattaat atctgtcatt
      841 gattgtctta ttatcagcta cccccgtatt aatcagagtt ttcatttatt tttaatgcat
      901 aatcagatgc cgcctcatca ttatcctcag tattatcata gccgaaaact tctcccggac
      961 acgggcgttc acgataatag tcatcgtcat agttttcatc gtcagataat gatatgtcgt
     1021 attctgaata tgattcacca caattataca tgaaatcatc tcctttgggg gggcaataat
     1081 tagtgagttt attcgaaaaa atgatgagcg ccagaccaat ccacccaact tttgattata
     1141 atcatgaaga tacttgtctc agtacaaatt gaatcaaact caatgcagca tgattcacct
     1201 ttatattctc tcagggaaac caaataccac tactcaacta ttaagaacaa aattcgggat
     1261 gtatgattat cagaagatac ctgcatccta aataatttta gacagatatt atcgttgtga
     1321 atcccccgcc acctttccgg tgcgcggggt tttgtctttt ttcaccggga atacatgtat
     1381 gaatccgtct gatgccattg aggcaattga aaaaccgctc tcctccctgc cttactcgct
     1441 ttcccgtcac atcctggaac atctgcgcaa actcaccagt cacgaacccg tgattggcat
     1501 tatgggtaaa agcggggccg gtaaatcctc actctgtaat gcactgtttc agggggaggt
     1561 caccccggtc agtgatgttc acgccggcac ccgggaagtg cggcgcttcc gtctgagtgg
     1621 ccatggtcac agcatggtta tcactgacct gcccggggtg ggcgagagcc gggacaggga
     1681 tgcagagtat gaagccctgt accgtgacat tctgcctgaa ctggacttgg tactgtggct
     1741 gattaaagcc gatgaccgtg ccctgtctgt ggatgagtat ttctggcgac acatactgca
     1801 ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacag agccctgcca
     1861 tgaatgggat atggcaggta ttcagccttc tcccgcacag gaacagaaca ttcgcgaaaa
     1921 aacagatgcg gtattccggc tgttccggcc cgtacatccg gttgttgccg tatcggcccg
     1981 caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc ccgaccatgc
     2041 cgccagtccc ctgatgaccc gcctgcagga cgagctgtgc acggaatctg tccgggggca
     2101 ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggtgg agagcgtctg
     2161 tgttgcctct gttgcacgca cagttctgcg tgccgtccgt gacacggtgg tctctgttgc
     2221 ccgcgcggta tggaactgga ttttcttctg aacagtctgc caggggaaat gtctgattac
     2281 attgcgttaa ccaactgaaa acatgataca aacacctcat gcccctcctg tttctcttgc
     2341 gtgactgctc tactgttaat agaataaaac gatcgataaa acaggtatcg atcgtttata
     2401 tcgatcgttt atagtggtta atggcctgcg aaaaggcagc tgaatctctt catcatgcag
     2461 aacggaattg cacacaacag actgatactt ctctgtctga tgaaggggga gagcgtttac
     2521 gcccgggacg tatcgctgtg cccgataact ctgttttgta cctgccggta tccacttttg
     2581 tgggtaccgg cttttttatt caccctctgt aaggaaaagc tgatgaaacg acatctgaac
     2641 accagctaca ggctggtatg gaatcacatt acgggcaccc tggtggtggc ctccgaactg
     2701 gcccgctcac ggggaaaacg cgccggtgtg gcggttgcgc tgtctcttgc tgctgtcaca
     2761 tcagtcccgg cactggctgc tgacaaggtt gtacaggcgg gagaaaccgt gaacgatgga
     2821 acactgacaa atcatgacaa ccagattgtc ttcggtacgg ccaacggaat gaccatcagt
     2881 accgggctgg aactggggcc ggacagtgaa gaaaacaccg gtgggcaatg gatacagaat
     2941 ggcgggatag ccggaaacac cactgtcacc acaaatggtc gtcaggtcgt gctggagggg
     3001 ggaacagcca gtgatacggt tattcgtgac ggcgggggac agagcctgaa cggactggcg
     3061 gtgaacacca cactgaataa cagaggcgag cagtgggtgc atgagggcgg ggttgccacc
     3121 ggtacaatta tcaaccgcga cggttaccag agcgttaaaa gtggcgggct ggcaacagga
     3181 accatcatca acaccggcgc agaaggcggc cctgattctg acaactcgta tacgggtcag
     3241 aaggtccagg gaacagcaga atccaccacc atcaacaaaa atggacggca gattatctta
     3301 ttttccgggc tggcccgtga cactctcatt tacgcaggtg gtgaccagtc ggtacacgga
     3361 agggccctga ataccacact gaatggcggt taccaatatg tgcacaggga cggacttgcg
     3421 ctgaacacgg taattaacga ggggggctgg caggttgtta aggcaggtgg cgctgccggt
     3481 aacaccacca taaatcagaa cggtgaactg agggtacatg ccggcgggga agccactgca
     3541 gtcacccaga acacgggcgg tgcactggtt accagtactg ctgcaactgt catcggcaca
     3601 aaccgtctgg ggaatttcac ggtggaaaac ggtaaggctg acggtgttgt tctggaatcc
     3661 ggcggtcgtc tggatgtact ggagagccat tcagcacaga ataccctagt ggatgacggc
     3721 ggtaccctgg cagtgtctgc cggcggtaag gcgacaagtg tcaccataac atccggtggt
     3781 gccctgattg cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt
     3841 attgatggca catccggtca ggccagcggc ctgctgctgg aaaatggcgg cagctttacg
     3901 gttaatgccg ggggacaggc tggcaacacc actgtcggac atcgtggaac actgacgctg
     3961 gctgccgggg gaagtctgag tggcagaaca cagctcagta aaggcgccag tatggtactg
     4021 aatggtgatg tggtcagtac cggcgatatt gttaacgcag gggagattcg ctttgataat
     4081 cagacgacac cgaatgccgc gctgagccgt gctgttgcaa aaagtaactc cccggtaacg
     4141 ttccataaac tgaccaccac gaacctcacc ggccagggcg gcaccatcaa tatgcgtgtt
     4201 cgccttgatg gcagcaatgc ctctgaccag ctggtgatta atggtggtca ggcaaccggc
     4261 aaaacctggc ttgcgtttac aaatgtcgga aacagcaacc tcggggtggc aaccaccgga
     4321 cagggtatcc gggttgtgga tgcacagaat ggcgccacca cagaagaagg tgcgtttgcc
     4381 ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc tgaaccgtga cagcgatgaa
     4441 gactggtacc tgcgcagtga aaatgcttat cgtgctgaag tccccctgta tacatccatg
     4501 ttgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt
     4561 gtaaac
//
LOCUS       DAZGXS010000220         4547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGXS010000220 DAZGXS010000000
VERSION     DAZGXS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..191)
                     /locus_tag="ACHKG5_004601"
     CDS             complement(<1..191)
                     /locus_tag="ACHKG5_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870557.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSA"
     gene            complement(730..816)
                     /locus_tag="ACHKG5_004602"
     tRNA            complement(730..816)
                     /locus_tag="ACHKG5_004602"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(780..782),aa:Leu,seq:taa)
     gene            complement(829..902)
                     /locus_tag="ACHKG5_004603"
     tRNA            complement(829..902)
                     /locus_tag="ACHKG5_004603"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(868..870),aa:Cys,seq:gca)
     gene            complement(956..1031)
                     /locus_tag="ACHKG5_004604"
     tRNA            complement(956..1031)
                     /locus_tag="ACHKG5_004604"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Gly,seq:gcc)
     gene            complement(1183..1731)
                     /gene="pgsA"
                     /locus_tag="ACHKG5_004605"
     CDS             complement(1183..1731)
                     /gene="pgsA"
                     /locus_tag="ACHKG5_004605"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0870558.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(1788..3620)
                     /gene="uvrC"
                     /locus_tag="ACHKG5_004606"
     CDS             complement(1788..3620)
                     /gene="uvrC"
                     /locus_tag="ACHKG5_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0870559.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3617..4273)
                     /gene="uvrY"
                     /locus_tag="ACHKG5_004607"
     CDS             complement(3617..4273)
                     /gene="uvrY"
                     /locus_tag="ACHKG5_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0870560.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4415..4531
                     /locus_tag="ACHKG5_004608"
     CDS             4415..4531
                     /locus_tag="ACHKG5_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0870561.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
BASE COUNT         1200 a         1182 c         1026 g         1139 t
ORIGIN      
        1 tatgcactgc gtccaccaca tctactacca ttccttgcat gattataagg acacgcacaa
       61 ttgcctggat aagattcaat ggattcttta attatttctt ttttgatctg agcatcagac
      121 tttcctgttg ctgcatatcc actaaaagat actaaacaaa ggcagattgc cattaataaa
      181 ccacatcgca taccgataca ctcaattact aaccaatcat agcccacatc ataaaatcga
      241 gatgaggagc acaatacctt taacaagaag gtgtcataac gataatatgt cgtgcaattc
      301 atcaaaaaaa agatctatat caaagagata gaactcttat cagtgcttca gacatcggca
      361 ttcgaggcag cagaaaaaca gtaaatctga cgaatttgcc ttttcccaag atgttgtcag
      421 attaaaaaga tagattagcg caggtacctg ttggtggatt gcaggaggaa aaataaagtc
      481 gagtggaaaa atcccagaaa aatttttgtt taacagaggt cctggttcac tatgcgaaat
      541 acggaggagc tgcagaagaa ttctgcttca tccttagagt ttaaacagca cgtgcactac
      601 cattgtttct gcatatcgcc acaccatcgc cactctaccg ccattgcaca aaatacaaat
      661 acgaaaaaac cacccgaagg tggtttcacg acactgctta ttgctttgat tttattctta
      721 gctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt
      781 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
      841 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
      901 ccaaattctt caggcctttc agccagacat ccgctgacgc cgatgtcttt taaactggag
      961 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
     1021 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     1081 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     1141 ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat caagcaaatc
     1201 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     1261 gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca gccatgccaa
     1321 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg
     1381 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     1441 cgttgccgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca gcaccatggc
     1501 gatagccacg agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg
     1561 gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     1621 gagcgcggcg gcaaacggcg accaagtgac aggcagataa aagaccaata caaagaatgg
     1681 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     1741 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     1801 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg
     1861 acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccggcgt
     1921 tttggcccga cgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt
     1981 ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc
     2041 agcgcgggag aatctggcgg caaactgaat ccctcacctt ccggctcaaa gaacagcgtt
     2101 tccagcccag ccttacgatc tgctcctttg gcaacaccaa gtagcagcgg atgattttta
     2161 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     2221 ccgtcgataa gtatcacatc cgggatctta ctgtcgtcga tggctttacc ataacgccga
     2281 cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgccagt aatgttatag
     2341 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     2401 tgttcaccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     2461 aacacgctgg caagtgctgt aagccgctgg tgaacggtag attgctgcga aagtttgctg
     2521 gttaaggccg tcgccgcatt ggtgcgcgcg agtttcaggt aacgcgccct atcgccacga
     2581 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     2641 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     2701 ccttgtaaat agaactggcc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca
     2761 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     2821 tggacacaag ccatacccgc atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta
     2881 ttggaaacga attgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt
     2941 gccgcttctt caaactccag attctgactg gcagtttcca tacggctaat gagttgcgta
     3001 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     3061 tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc
     3121 agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaaaatc
     3181 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     3241 tcacctttgg catgctttgc accacgatgc atcgccagac gcgggtgggt atcgccactc
     3301 aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag
     3361 agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca
     3421 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg
     3481 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     3541 ccagcatcgt acatgcgata aacgccaggc tggctggtta cggtttttaa aaacgctttt
     3601 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     3661 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     3721 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     3781 ggcccttcgt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     3841 tttctgtttt ttctggttcg atctggctta atgccatttg ttgagcgatg tcagaagcaa
     3901 tgtaacgctg cccagaatag acagaacgga tcgcactcac gacttcctgc ggagccgcgc
     3961 ctttgctgag gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg
     4021 tatggacggt aagcatgatg atttttacat cagctgtgga acgcgcgatt ttacgcgtcg
     4081 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     4141 cccggcacca cttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct
     4201 ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     4261 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     4321 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     4381 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     4441 accggatcgt ttcacagtaa gccctgcgac gaaatgtgac aaaaattacc tttattcagc
     4501 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaa
//
LOCUS       DAZGXS010000221         4476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGXS010000221 DAZGXS010000000
VERSION     DAZGXS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..887
                     /gene="sepL"
                     /locus_tag="ACHKG5_004609"
     CDS             <1..887
                     /gene="sepL"
                     /locus_tag="ACHKG5_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFP0870562.1"
                     /translation="ETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIK
                     ELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLG
                     EDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREK
                     HLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKDI
                     ILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQR
                     ENALLMIGKVIDYKEEII"
     gene            946..1524
                     /gene="espA"
                     /locus_tag="ACHKG5_004610"
     CDS             946..1524
                     /gene="espA"
                     /locus_tag="ACHKG5_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP0870563.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            1537..2661
                     /gene="espD"
                     /locus_tag="ACHKG5_004611"
     CDS             1537..2661
                     /gene="espD"
                     /locus_tag="ACHKG5_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFP0870564.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            2682..3620
                     /gene="espB"
                     /locus_tag="ACHKG5_004612"
     CDS             2682..3620
                     /gene="espB"
                     /locus_tag="ACHKG5_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP0870565.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            3627..4034
                     /gene="cesD2"
                     /locus_tag="ACHKG5_004613"
     CDS             3627..4034
                     /gene="cesD2"
                     /locus_tag="ACHKG5_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP0870566.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            4070..4291
                     /gene="escF"
                     /locus_tag="ACHKG5_004614"
     CDS             4070..4291
                     /gene="escF"
                     /locus_tag="ACHKG5_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP0870567.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            4297..>4476
                     /gene="escG"
                     /locus_tag="ACHKG5_004615"
     CDS             4297..>4476
                     /gene="escG"
                     /locus_tag="ACHKG5_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFP0870568.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKND"
BASE COUNT         1350 a          801 c         1062 g         1263 t
ORIGIN      
        1 ctgaaacgat tgaagggcta agcctgggat atcgcaaagg tagcgcaagg aaagaagagg
       61 aaggctcgac aatcgagaag ctgctcaatg atatgcaaga gctgcttact cttaccgata
      121 gtgataaaat aaaagaacta tcattaaaaa acagcgggct gctggaacaa cacgatccta
      181 ctttggcgat gtttggcaac atgccaaagg gggaaattgt tgcgctaata tcttctttat
      241 tgcaatctaa gtttgttaag attgaattaa aaaagaaata tgccaggtta ttattagatt
      301 tattaggcga agatgattgg gagctggctc tgctttcctg gttaggggtg ggtgagttaa
      361 atcaggaagg tatccagaag atcaagaagc tttatgaaaa ggctaaggat gaggattctg
      421 aaaatggcgc ctctttactt gactggttta tggagattaa ggatcttccc gagcgtgaga
      481 agcacttaaa agtcattatt agggcgctgt cgttcgatct ctcttatatg tcttcttttg
      541 aagacaaagt aaagacatct tcaattatta gtgatttatg cagggtaatc atttttttat
      601 cacttgataa ctatgcagat attatttcga tctctattaa gaaagataaa gatatcattt
      661 taaatgaagt gttatcgatt attgaacatg tctggctaac agaagactgg ttgctggaga
      721 gtccttctcg ggtatcgatt gtcgaagata aacatattta ttattttcat ttattgaaag
      781 acttttttac atcattacca gatgcttgct ttattgatag tgagcagaga gagaatgcat
      841 tattaatgat tggtaaagtt atcgactata aggaggaaat tatttgatat taattactca
      901 ataatttttt tgttttcctg agaaaaatta tcaagaggta tatagatgga tacatcaaat
      961 gcaacatccg ttgttaatgt gagtgcgagt tcttcgacat cgacgatcta tgacttaggt
     1021 aatatgtcga aggatgaggt ggttaagcta tttgaggaac tcggtgtttt tcaggctgcg
     1081 attctcatgt tttcttatat gtatcaggca caaagtaatc tgtcgattgc aaagtttgct
     1141 gatatgaatg aggcatctaa agcgtcaacc acggcacaaa agatggctaa tcttgtggat
     1201 gccaaaattg ctgatgttca gagtagcact gataagaatg cgaaagccaa acttcctcaa
     1261 gacgtgattg actatataaa cgatccacgt aatgacataa gtgtaactgg tattcgtgat
     1321 cttagtggtg atttaagcgc tggtgatctg caaacagtga aggcggctat ttcagctaaa
     1381 gcgaataacc tgacaacggt agtgaataat agccagctcg aaattcagca aatgtcgaat
     1441 acattaaatc tcttaacgag tgcacgttct gatgtgcaat ctctacaata tagaactatt
     1501 tcagcaatat cccttggtaa ataaccggag ataactatgc ttaacgtaaa taacgatacc
     1561 ctgtctgtaa cgtctggggt taataccgcc tcgggtactt ctggtattac tcaatctgaa
     1621 acgggtttat cgctggattt acaactggtt aaatccatga actcgtcagc aggctggaca
     1681 gaaagtagcc ctttaccgac gccgccggca ggtcactcat tagtgacgcc ctctgctgct
     1741 gaggatgtcc ttagtaaatt gtttggtggt attagtggtg aggttacaag tcgcactgag
     1801 gaggcagagc cacagcgcac aagctatccc tatctctctc aggtgaatac cgttgaccct
     1861 cagcaaatga tgatgatggt cactctgtta tccctggata cttccgcgca gaaagtctcg
     1921 agtctgaaaa actctaacga gatttatatg gatgggcaaa ctaaagcgct ggagaataaa
     1981 acgcaggagt ataaaaaaca gctcgaagaa caacagaaag ccgaagagaa atcacaaaaa
     2041 agtaaaattg ttggccaggt ctttggttgg ttgggcgtcg cattaacagc cgttgccgct
     2101 gtttttaacc cagcactctg ggctgttgtt gccattggtg caacagcaat ggcactgcaa
     2161 acggcagtcg atgtaatggg ggaaaatgcc cctcagggat taaagactgc agcacaggtc
     2221 tttggcggaa tatctatggc cgcaagcatt ctgacagccg gcgttggcgg ggtgtcttca
     2281 ctgttatcta aatttggtaa tgttgctaac aaaattggct caagcgttgt aaaagtcgtt
     2341 gagaaggcgg cagaagcgct ggttaaaaac gtttttgcaa aaatttcgac agtggctgag
     2401 ggcgttacga acggtattcg ttctgccggg acaactgcgt tgaataatga ggctgcgcaa
     2461 ctccaaatgt tgtctcagtt agctgctttc gcggtgcaaa acttaactcg acagagtgaa
     2521 agcttaggtg agagtgcgaa gctcgagctg gataaagcgg caagcgagtt acaaaatcag
     2581 gcgagctatt tacaaagtgt ttctcaactg atgtccgatt cagcacgggt aaatagtcgt
     2641 attgttagtg gccgaattta attattaaag agaattttat tatgaatact attgataata
     2701 ctcaagtaac gatggttaat tccgcttcgg agagtacgac cggcgcttcc agtgcagttg
     2761 ccgcatctgc tttatcaatt gattcatctc tgcttactga tggtaaggtt gatatttgta
     2821 agctgatgct ggaaattcaa aaactcctcg gcaagatggt gactctattg caggattacc
     2881 aacaaaaaca attggcgcaa agctatcaga ttcagcaggc cgtttttgag agccagaata
     2941 aagctattga ggaaaaaaaa gccgcggcaa ccgctgcttt ggttggcggg attatttcat
     3001 cagcattggg gatcttaggt tcttttgcag caatgaacaa cgcggctaaa ggggctggtg
     3061 agattgctga aaaagcaagc tctgcatctt caaaggctgc tggtgcggct tctgaggttg
     3121 caaataaagc tctggtcaag gctacggaaa gtgttgctga tgtcgcagag gaggcatcca
     3181 gtgcgatgca gaaagcgatg gccacaacaa cgaaagcagc cagccgtgca tctggcgttg
     3241 cagatgatgt tgcgaaagcc tctgactttg ctgaagatct tgcagacgcc gccgagaaga
     3301 caagcagaat caataagttg ttgaattccg tagataaact gaccaatacc acagcatttg
     3361 ttgccgtgac cagtcttgct gaaggtacga aaacgttgcc aacaacaata tctgagtccg
     3421 tcaaatcgac tcatgaggtt aatgaacaac gtgcgaagtc gctggaaaac ttccagcagg
     3481 ggaatctgga gctgtataaa caagacgttc gcagaacgca ggatgatatc acgactcgtc
     3541 tgcgtgatat aacgtccgct gtccgcgatc tccttgaggt ccagaatcgt atggggcaat
     3601 cgggtcgctt agctgggtaa ttgatcatgg tcgatacgtt taatgatgaa gtgtttaatt
     3661 actatcttga acaaaaaggg tacacaatac agaaggagtt tctttgtggc agtgcctttt
     3721 ttatcggatg gcggattgag acaccttttt tttcattagc gtacagactg gatgaacaag
     3781 aactgatttt gtgctctttt gaagcacgta accaaacagg gcttaacggc cctgttttat
     3841 cgctgactca tttgctcgaa gagttgtatc accatttttc gggtattaag aaaatcagcg
     3901 cgatgaaatc taagattggt tcagattcag aacgtcaaaa gcgcgaagag ttgtttaatt
     3961 acttcatcag aaagggtgcc gtgcagcaag aaacagaaga cggaatttgg ttcgtaatga
     4021 acgtaaatag ttaaaagttt ttattttatt aacttctgag ggaaatttaa tgaatttatc
     4081 tgaaattact caacaaatgg gtgaagtagg taaaacgctg agcgattctg tgccagagtt
     4141 acttaatagc accgatttgg ttaatgaccc tgaaaaaatg ttagagttgc agtttgcggt
     4201 tcagcaatat tctgcttatg ttaacgtaga aagtggaatg ttgaaaacga taaaagatct
     4261 ggtctcaacc atttctaacc gtagttttta attttaatgg ttaatgatat ttctgctaat
     4321 aagatactgg tgtgggcggc tgtagctgcg gcaaaccata agcttcccaa atatgcggaa
     4381 gctatcctga atgtattccc acaaattata cctgataaaa aagatatcgc acatttagaa
     4441 tttattatct tatttggatt aaatagaaaa aatgat
//
LOCUS       DAZGXS010000222         4447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGXS010000222 DAZGXS010000000
VERSION     DAZGXS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..846)
                     /locus_tag="ACHKG5_004616"
     CDS             complement(<1..846)
                     /locus_tag="ACHKG5_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP0870569.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIV"
     gene            complement(839..1984)
                     /locus_tag="ACHKG5_004617"
     CDS             complement(839..1984)
                     /locus_tag="ACHKG5_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFP0870570.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            complement(1989..3692)
                     /gene="sctC"
                     /locus_tag="ACHKG5_004618"
     CDS             complement(1989..3692)
                     /gene="sctC"
                     /locus_tag="ACHKG5_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFP0870571.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(3689..4438)
                     /locus_tag="ACHKG5_004619"
     CDS             complement(3689..4438)
                     /locus_tag="ACHKG5_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870572.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT         1322 a          958 c          738 g         1429 t
ORIGIN      
        1 aacaatgaca aaagaattac ttaataactg ggacattata ttttgcccca tattatcact
       61 atcgccattt acgcgcgtaa cgataaatcc tgcgctaata gcaattaata aagctggaat
      121 ttgagaaaca agaccatcac cgacagtaag aattgtgtag acagtcaacg ctgttgagaa
      181 atccattcca ttttggccta ctcctactga tagcccaccg ataatattaa caaagataat
      241 aataatgttt gcaatagcat cacctttgat aaatttcatc gcaccatcaa aggaaccata
      301 caactggctc tctcgttcta atacactacg acgttctttt gctaaatctg catcaatgat
      361 cccggcgcgt agatcggcgt caatgctcat ttgtttacct ggcataccgt caagcgaaaa
      421 gcgggcagca acttcagcca cacgttccga acctttagta atcacgataa actgaacaat
      481 agtaacaatg gagaaaataa caaaaccaac taccaggcta tctccaataa cgaactcacc
      541 aaaactcgtt ataatttctc cagcatccgc ctcaagaagt ataagtcgac tggtactgat
      601 cgccagcgcc aaacgaaata acgttgtgat aaggaggatt gatggaaatg aagaaaagct
      661 taagatccta tcaacataga atgatcctaa aaatacaagt attgccaatg ttaagttaag
      721 cccaatcaaa aagtcgatta aataggtggg caatggaatt atgagcatgg cgataatcat
      781 caccattaat ccaagaatta ttaattctgg atgactcctt aaccctacca aaactttgtt
      841 aaacattgaa catcaccttc ttccatggtt ttttattttg ttttttgtta cagttttgtg
      901 tatcaaagag ctctgctgca aatgttactt ttattgcctg tttacaacac tccctaatct
      961 ctaataattg ttcattatta ataaaaagtg aaaatggaag atttgtacat ccactgtaaa
     1021 tcgccttcag gagtaacgtt ttgtctttat aattagcatc tgcgggaagg gtcaccagtg
     1081 cattgttcag gagttcctct gcagtctcag gaaattttat taacgatatc agaaaaaata
     1141 tccagtaatc ttctgcgctg ataaattttt taattattgg gttattcatt aaagttttca
     1201 gaaataaagc ctctgccgac tgaagagaca atagttgtga caacttattg agaaacatac
     1261 caaattccaa tgatgaacaa ctcgcatcaa gtgcatttat atctgagaaa agcgtctctt
     1321 tgataaattt tgtcaccaac attctatttt gatagccata actaccaatc cattcgacat
     1381 aagtatctac agcatcgtct tctgccatta aaaattgccg ataactcgcc cttagtaatt
     1441 ttggatttaa tgacagcgcc tggccaaata actttgcttt taatgccgag ttaattccgc
     1501 tattaatgaa ttttttagta gtctcttgat taacttttct aagtaatgct tcagcgttta
     1561 gccggacatt ttctgatatt tgttttttac gtaaaattgc acgtaacaca actaccagat
     1621 cgctttcatc tgggaataat tttttagcaa agctatacac ttcatagtca tgggtagttg
     1681 cagccttaag cgcctccata actttgtcgg ttttttctat tggctggtct tccaggacat
     1741 attcttctcc gtccagaaaa ttggccagtt gttcttcgta aatttttttc ttcataaact
     1801 gcgttaacgc tgctgacatc tcgtcagtag cttgtaagaa ccgagcttct ttacctgcgg
     1861 gatcatccct cattttaggc atttcagctt caatgtcttc attatcttct gtgataatgt
     1921 cctctaagcg agaaacttcc ctggctcttt ctaatacacc gacatgctct acatgaatcg
     1981 ccatttcact atcctccgac gacttcacga ttcaggtaag tttttatcca tttctgcagt
     2041 aattcatcgt tgatttcttt attttttgcc atttgctgtg taatcgctct ggcatcaacc
     2101 gcagcctcat tcgcattatt catcatgcgt tcatcgatct ctcttggctc aataagaaag
     2161 acacgaacga tgttattcgc atttgttcct tcataaccaa ataatttacc aataaatgga
     2221 atgctgccta atattgggat cttgcgactt tcataggtat tcgtatcgcg tgtatatccc
     2281 ccaatcaaaa gacttttccc ttggggcacg cgtgcaatag tacttataag tgttctacca
     2341 acctgaggca gatcatcaac attggtcttg tcggaattga tttcatttcc atcttcaata
     2401 ttcaagagta attcaatttg atttcgcgca gcaaatcgtg gtagcacgct aatcatcgta
     2461 ccataggtta cttcatccaa ttccgcagta cgctcgccga ctaatttagt atagaaagtg
     2521 cggttattat caaatatagc aggaatattc tcttgtgtta aaacgacggg acgagcaaca
     2581 acggcggctc ttcttttttg tgcaagtgcc tgtattgttg caataaactg tgtcccatca
     2641 agggtactaa ttgaacctga attattaaat gatgcactta agctggatcc aatttttatc
     2701 gtgcccgacc agtcagcacc tagctgttct agatcagtct tatcaatatc tattatccat
     2761 aaagataatt caatgtgtcg ctttggaata tcaagcgtag ctactaactt ctcgataaaa
     2821 tcaacctgag atactgttcc tttaacaagt aaactgttgg tatcaggata tgcaataatt
     2881 tgtatatcgt cagtctctgg cgctcctgct gtcgatatta ttttctctac ttgtaggtca
     2941 tcatcttctt cagccccagg aaaaggaggc atacgtgata cgttttttag ttgttgacga
     3001 cttgaaattt cagaaagtac gttcacccct gtgctttgct taatgttatt atttaacaac
     3061 gtcgatagta cttttgccat gccaggaata actatctttt cgccacgtaa ctcataagtc
     3121 cggtcattaa cgaatgtgtt gaccagatga attattccta ctttatttcg accgatctca
     3181 atgccatcgc tgttttgctc cattaatttc gcagcattaa cgaccaggtc aacataaacc
     3241 ggtggccccg atacatagaa agtaccattg ccgtcccctt taatttcata acgagagtta
     3301 tacagacccg atttttttaa gaaattatta aactcattcg tcgatacctt tctcagatta
     3361 atcaatgcat tacgcatctc tgatgagtca tagatataaa tagctttgcc atcatcatac
     3421 caaataagcc ccatctgtgc cgacaggcgt tcaatcaagg catagggttc tgttaaatca
     3481 aaatttccgc tgatctgttt cttcatggca agcttactaa ctattgtagg cttacccgca
     3541 tatgatgaaa ccgtttcaaa aaaactacgt agattttctt tatttgctac gtatccattc
     3601 gctgctgcgg catatgcacc aggagtcaat aatccattaa aaatgcataa gactgaaatt
     3661 aatataatag taatgcgtaa ttttattttc ataacttttt acatagttct ctcggtgttt
     3721 ttcctaaccg gctttttact tcggctgaaa agtgtgatga ggacgagtaa ccatatttat
     3781 gcgcaatagt tgtcatatca ctgtttcctt caatgatttc caacacggct ctggcaactc
     3841 tccagccaca taattccgtt ttaactttac cacccaatgc ataactacat aaacggcgaa
     3901 aatgtgaata cgatacgccg taaagctcac caagatcata gagtgaagtc tgtatgagcg
     3961 attgcgataa taaataacga accagtttat agcattctgt ttttcttaga tgctgaaaca
     4021 ggatttcatt aatattatcg ctgctcagat aagtggctaa aaaccaggct tcacttgcaa
     4081 ctttactgaa tagcacaact tctgatgcaa agggcaaggc atcagcaaac aattcatggt
     4141 gttccctttc cacactcccc gttgattcat ctataaaagc taataattta ctcaggatat
     4201 caatgcgaag tggatgataa attactttcc ctgcagttac ctcaatttgg cttcggtcaa
     4261 tgagcagtaa tatatcttcc actaccatta atttttgatg gttattacca atcgtaataa
     4321 caagggttac atccttgttt tcatttcccc gtaagaacca catttcaggg tccagtaggg
     4381 ttaacttctg tccttgaagc aaaacattca ttggaagtaa cagcccttct tcaatcatgt
     4441 ttcatct
//
LOCUS       DAZGXS010000223         4431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGXS010000223 DAZGXS010000000
VERSION     DAZGXS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            56..899
                     /locus_tag="ACHKG5_004620"
     CDS             join(56..402,401..899)
                     /locus_tag="ACHKG5_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFP0870573.1"
                     /translation="MTRYDLPDEAWTIIKPLLPPEPATRQAGRPWAEHRKIINGMFWV
                     LCSGGPWRDLPERYGPWKTVNNRFNRWSKSGVINIIFNRLLSRLDANGFVDWSATALD
                     GSNIRALKCAAGAHKKHPDIDEDNGLGRSRGGFGTKIHLATDGSGLPLNIVLSPGQSH
                     ESQFAQRLLDGIGVQRQNGCIKRRGHVVLADKAYSGHTLRNELKNKGLKAVIPRKSNE
                     KMASVGRARLDRDAYRNSNVVERCFGHLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            complement(921..3572)
                     /locus_tag="ACHKG5_004621"
     CDS             complement(921..3572)
                     /locus_tag="ACHKG5_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrlF family AAA-like ATPase"
                     /protein_id="HFP0870574.1"
                     /translation="MSVSWTKADFFKCALQVNPAGYSKYRGKESLSEDTYNQKLLESC
                     LEAGIQIVGVADHGAVNAVDRIRQLFKEHGIVVFPGFEICSSEKIHFVCLFDENRTVQ
                     ELERCLSNLGYENPDDCISPSRLSAIQIIEKVECLGGFIFAAHSTNDNGVLKCRMNHI
                     WKERGLLAAQIPGTVDDLKGVEDDFYRKAILNKLPDYRRERPIAIINAADVAQPEEIK
                     TPGASCLIKMTRPCFSSFKQAFLDPESRVRLNADKPHCYASAIENIHFTGGYLDETNI
                     TFSDHLNAVIGGRGTGKSTLLEAIRFALGKQPFGDSAQKQHSAIIKTNLGNGTLVQLQ
                     VRSAMMQGRTFTISRKFGGKSIVTDSEGKVSSYTPDDLLPRLELYGQNEIYEMTRDIH
                     SRNRLVERFLEGDHSKYDVIIDKVLSRLEESSITILNALEQKAEIELEVARLPKLLEQ
                     AEQFKQLGLDEKLKIVPLLEKEKQLSLRHQEELSRVKDVLQKLKDSLPDLAYLDDGVI
                     NVMPHHSLFLQQRDVLQRAQEQLSTLVQHIDETLQRSFAELAPLQLELSDLIGAEEAQ
                     LEKAFKDIPASQGKSGRQIGAEYQILLRQIASIRPKATELENWQKQLDELYQQRKRHL
                     LELIQARAQRSAALAKSVKRLNRRLDEKVRLNLQPEGNRQPLLHFLEQCNLEGVGSRR
                     LAWVLEHDFSPENLVESIRLGEQALHNAGWGITPTVIQALLRLSEKQLLELEALSLPD
                     TMQIELNVMHDGGGVLWRPIDELSTGQQCTAVLHLLLLDNKDPLILDQPEDNLDNAFI
                     AERIVTELRRAKLSRQFLFATHNANIPVFGDAEWIGVLSVEEGKGRILPEQQGAIDLP
                     EIQCLAANILEGGQSAFNQRREKYGFK"
     gene            3939..4130
                     /locus_tag="ACHKG5_004622"
                     /pseudo
     CDS             3939..4130
                     /locus_tag="ACHKG5_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065554.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1213 a         1072 c          947 g         1199 t
ORIGIN      
        1 tgtcccgtaa tagtttgagc tatagtaacg gtctgtttcc acaacatatt gaaccatgac
       61 tcgctacgac ctccccgatg aagcatggac catcatcaag cccttgttgc cccctgaacc
      121 tgccacacga caggccgggc gcccatgggc agagcatcgt aaaatcatca acggcatgtt
      181 ctgggtgttg tgttccggcg gaccatggcg cgatttaccc gaacgatatg ggccatggaa
      241 aaccgttaat aaccgcttta acaggtggtc aaagtcaggc gtgattaaca tcatttttaa
      301 cagattgctt tcacgacttg atgcgaacgg ctttgttgac tggtctgcca cagcgctgga
      361 tggcagtaat atccgggcgc taaaatgcgc cgccggagct caaaaaacat cccgatatcg
      421 acgaagataa tgggctgggt cgctctcgag gcggttttgg caccaaaatc catctggcaa
      481 cagacggaag cggcctcccg ttaaatatcg tgctaagccc cgggcagtct catgaaagcc
      541 agtttgcgca acgacttctg gacggcattg gcgttcagcg tcagaacggt tgcataaaac
      601 gccgtggtca tgttgtgctg gccgacaaag cgtactccgg acacacattg cgcaacgagc
      661 tgaaaaataa aggcttaaag gcagtcatcc cccgaaaatc aaatgaaaaa atggcatcgg
      721 ttggacgagc acggcttgat cgcgatgcgt atcgcaatag caacgtcgtt gagcggtgct
      781 ttggtcacct gaaagaatac cgccgcatcg ctacacgtta cgacaaaacg gcaagaaatt
      841 atctggcgat ggtgaaactg ggctgcatcc gactctttta tcaacggtta cgtaattaag
      901 ggagacagcc taattctttg ttatttgaat ccatattttt cccggcgctg attaaaggca
      961 ctttgaccac cttcgaggat attcgccgcc agacactgga tttcaggtag atcaatcgcc
     1021 ccttgctgtt caggaaggat acgccctttc ccctcttcca cactcaacac accaatccat
     1081 tcagcatcac cgaaaactgg tatgttagcg ttatgtgtgg caaataaaaa ctgccgggac
     1141 agcttcgccc tacgcagttc cgtcactata cgctctgcaa taaatgcatt atcaaggtta
     1201 tcctcaggtt gatcgaggat cagtgggtcc ttattatcta acaacaataa atgcagtaca
     1261 gcagtacact gctgcccagt cgatagctca tcgatagggc gccagagtac gccaccaccg
     1321 tcatgcatga cattcagctc aatttgcatc gtatccggta atgataatgc ttccagttct
     1381 agaagctgct tttcgctcaa ccgcaataat gcctggatga ctgttggtgt aattccccac
     1441 ccggcattat gtaatgcttg ttcaccaagg cggatactct caaccagatt ttccggtgaa
     1501 aaatcatgct ccaataccca agcgaggcgt cgtgagccta ccccttccag attacattgc
     1561 tccagaaaat gcaacagggg ttgacggtta ccttctggct gcaaattgag tcgcactttt
     1621 tcatccagtc ttctatttaa gcgcttgacg gattttgcca atgcagcaga acgttgagca
     1681 cgagcctgaa taagttccag aaggtgtctt ttacgctgct ggtacagctc atcaagttgc
     1741 ttttgccagt tctccagctc cgttgcttta gggcgaatag aagcaatttg acgtaacaat
     1801 atttgatact ctgcgccaat ctgtcgccca ctttttccct ggctggctgg aatatcttta
     1861 aaggcttttt caagctgagc ttcttctgca ccgattagat ctgataactc aagttgtaaa
     1921 ggagcaagtt ccgcaaatga ccgctgaagg gtctcatcaa tatgttgaac tagtgttgac
     1981 aattgctcct gtgctcgttg gagtacatca cgttgctgga gaaaaagaga gtggtgaggc
     2041 atgacattta tcacgccatc gtcaagataa gcgaggtcag gcaggctgtc ttttagtttc
     2101 tgaagaacat ccttcaccct agataattct tcctgatgac gtaagctaag ctgcttctct
     2161 ttttccagta aaggaacaat tttgagtttt tcatccaagc caagctgttt aaattgctct
     2221 gcctgctcca gtagtttcgg caatcgtgct acttcaagtt ctatctctgc cttttgctca
     2281 agagcattta ggatagtaat gctactctct tccaagcgtg acaagacctt atcgattatc
     2341 acatcgtact tgctatgatc tccttccaag aatctctcaa ccaaacgatt acgactatga
     2401 atatcgcggg tcatttcata aatttcgttc tgcccataca attccaacct aggaagtaaa
     2461 tcatctgggg tataagatga aactttccct tcactgtctg ttacaatgga tttcccgcca
     2521 aatttgcgac ttatggtaaa ggtacggcct tgcatcatag cagagcgaac ctgtagttga
     2581 accagcgtac cgttccctaa gttagtttta ataattgcac tatgttgctt ttgtgctgaa
     2641 tcaccaaaag gctgtttacc cagagcaaag cgaatggcct ccagcaaagt cgatttacct
     2701 gtcccacgcc cacctataac agcattgagg tggtcagaaa aggtgatatt ggtttcgtcc
     2761 agatatccac cagtaaaatg aatattttca attgccgaag catagcaatg tggcttatct
     2821 gcgttgagac gcactcgtga ctctggatct aaaaatgcct gtttaaatga actgaagcat
     2881 ggccgagtca tttttatcag gcaggatgcg cccggagttt taatttcctc tggttgggca
     2941 acatctgccg cgttgataat ggcaatagga cgctcgcgac gatagtcagg aagtttgttc
     3001 aggatagctt tgcgataaaa atcatcctca actcctttca agtcatcgac agttcctgga
     3061 atctgcgccg ccaacaaccc acgttctttc catatatgat tcatacggca tttcagcacg
     3121 ccattatcat tcgtgctatg tgcagcgaaa ataaatcccc ccaaacattc tactttttca
     3181 ataatctgta tggcgctgag ccttgaaggg gaaatgcaat catcgggatt ttcatacccc
     3241 agattgctca agcagcgttc caattcttgt actgtccggt tttcatcaaa caaacaaacg
     3301 aaatgaattt tttcgctcga gcagatctca aatccaggaa aaacgacaat accatgttcc
     3361 ttaaagagct gacgaatcct gtctacagca ttaacagcac catggtccgc aacaccgaca
     3421 atctgtatac cagcttcgag gcaggactcc aacagcttct gattgtaggt atcctcgcta
     3481 agtgattctt tcccgcgata cttactgtaa cccgcaggat ttacctgcaa agcgcactta
     3541 aaaaaatcag ctttagtcca gctcacagac atcgtcaggc tccatatctt tccaatagat
     3601 ccagtagatt actacgcgta gcaaataata cgcttaaaat cgctgcatcg cattgataaa
     3661 tatgttattc gctggctgcc tgtcgtacaa gcagtagcaa agaggctgac ttattgccgt
     3721 taccgctggc aaacaaattg tacctgcgtc agagctagca tcttcgatgt agcaaataaa
     3781 aaactaaaag catctgcgtg gcaagatatt aatgccaaat gaatgtactg catcatgtgt
     3841 cagagtgtgc tgatacatga acgtaaatct gctttaccgc ccccaatggt cgcataatag
     3901 gaaagtaaaa agcggtgatg atctacctag catggaggga aagtagagct cgcgacgttg
     3961 agtatgcagc cctgtaccat gaattacttc cccgcctcgt tctagtgctg tgactgattt
     4021 aggctgatga acaggctctg acggcggatg agcatttcaa tttttaggta atagacgagg
     4081 cgtaccatca taaggggctg tttgttatta ttcagtcaga taagactgag ctaaataaat
     4141 ctttatcggg agccagaatc gttctggctc ttcgagatgt tcctgcaaaa acttaatgcc
     4201 tttttgcttc aaaccacggt gccaagtagt gtacttcttg taaaatttca gtattcagta
     4261 cgataaagca ccgccacgga tagccttcat cattgattta atcaatgttc catttgttca
     4321 tcacgtcgca gaccaagaca gccccactag aaatggaatc aggattgctt acatatgaca
     4381 aaaaacgata ttcattaaaa atgatattta cttacaaata gttacgcatc c
//
LOCUS       DAZGXS010000224         4398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGXS010000224 DAZGXS010000000
VERSION     DAZGXS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHKG5_004623"
     CDS             complement(<1..209)
                     /locus_tag="ACHKG5_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0870575.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKG5_004624"
     tRNA            complement(324..399)
                     /locus_tag="ACHKG5_004624"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKG5_004625"
     tRNA            complement(406..480)
                     /locus_tag="ACHKG5_004625"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKG5_004626"
     tRNA            complement(597..681)
                     /locus_tag="ACHKG5_004626"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKG5_004627"
     tRNA            complement(690..765)
                     /locus_tag="ACHKG5_004627"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKG5_004628"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKG5_004628"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0870576.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKG5_004629"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKG5_004629"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0870577.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKG5_004630"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKG5_004630"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0870578.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4231..4346)
                     /gene="rrf"
                     /locus_tag="ACHKG5_004631"
     rRNA            complement(4231..4346)
                     /gene="rrf"
                     /locus_tag="ACHKG5_004631"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1115 a         1096 c          992 g         1195 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggcctttcgc tttatttgat gcctggcagt tccctactct cgcatgggga
     4261 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
     4321 gggaccaccg cgctaaggcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
     4381 gatttaatct gtatcagg
//
LOCUS       DAZGXS010000225         4313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGXS010000225 DAZGXS010000000
VERSION     DAZGXS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            136..4038
                     /gene="espP"
                     /locus_tag="ACHKG5_004632"
     CDS             136..4038
                     /gene="espP"
                     /locus_tag="ACHKG5_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP0870579.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT         1315 a          940 c          970 g         1088 t
ORIGIN      
        1 tttgttatcc gtatgataac aaacacataa aggagtaaac atttatctgg acgatgatta
       61 caatgttcac gactgtcaga gacacacctc tgatacatta gaaaaaccaa tgtttccctt
      121 aaaaatggag cttatatgaa taaaatatac tctcttaaat acagccatat tacaggaggg
      181 ttaatcgctg tttctgaatt atccggcaga gtatcatcaa gagcaactgg taagaaaaaa
      241 cacaaacgca tacttgcatt atgtttttta ggcttattac aatcctcata ttcttttgcg
      301 tcacagatgg atatttcaaa tttctacatc cgtgactata tggattttgc acagaacaag
      361 ggcatatttc aggctggcgc aacaaatatt gaaatagtga agaaagatgg ctccaccctg
      421 aaactaccgg aagtaccatt tcctgacttc tcaccggttg caaacaaagg gtcaaccaca
      481 tctattggtg gtgcatacag tatcacagcc acacacaata cgaaaaacca ccactcagtt
      541 gcgacgcaaa actgggggaa cagcacgtac aaacaaactg actggaatac ttcacatcct
      601 gattttgcag tatcccgact tgacaagttt gttgttgaga cccgaggtgc gactgaaggc
      661 gcagatattt cgttatcaaa acagcaggca cttgaacgtt acggggttaa ttataaagga
      721 gaaaagaaac ttatcgcatt cagagccggc tctggtgtgg tatccgttaa aaaaaatgga
      781 cgcataactc catttaatga ggtttcttat aagccagaaa tgttaaatgg ctctttcgtt
      841 cacattgatg actggagtgg atggctgata ttaaccaaca accagtttga tgagtttaat
      901 aacattgcct ctcagggtga cagcggttca gcactgttcg tctatgataa ccaaaagaaa
      961 aagtgggttg tcgctggaac tgtctggggg atttataatt acgccaatgg caaaaaccac
     1021 gcagcataca gtaaatggaa ccagacaacc attgacaacc tgaagaacaa gtattcttac
     1081 aacgtggata tgtcaggggc tcaggttgca accattgaaa atggaaaact gacaggcact
     1141 ggctcagaca ccaccgatat aaaaaataag gacttaatat ttactggcgg tggagatatc
     1201 ctcctgaaat cctcttttga taatggtgct ggcggtcttg tctttaatga taaaaagacc
     1261 tatcgagtaa acggggatga tttcaccttt aaaggtgccg gtgttgatac aagaaacggc
     1321 agcaccgttg agtggaatat ccggtatgat aataaagaca accttcacaa aattggtgat
     1381 ggcacattag atgtccgaaa aacccagaac accaacctga aaacaggtga gggtcttgtc
     1441 attcttggag ctgaaaaaac attcaataat atctacataa ccagtggtga tggaactgtc
     1501 cgactgaatg cagaaaatgc actgtctggc ggtgaataca acggtatttt ctttgcgaaa
     1561 aatggcggaa ctcttgacct gaacggatat aatcagtctt tcaataaaat tgctgcaact
     1621 gattcaggtg ctgtaataac caatacgtca accaaaaaat ccattttatc cctgaataat
     1681 actgctgact atatctatca cggcaacata aacgggaatc tggacgtact tcagcatcat
     1741 gagacgaaaa aagagaaccg tcgtcttatt cttgatgggg gcgtggacac aacaaatgat
     1801 ataagcctgc gtaatacaca actgtccatg cagggacatg ccactgaaca tgccatttat
     1861 cgggatggag ctttctcttg ttcactacca gctcctatgc gctttttgtg tggcagtgat
     1921 tatgttgcag gaatgcaaaa tacagaagct gatgctgtaa aacaaaacgg aaatgcctat
     1981 aaaaccaaca atgctgtctc tgatttatcg cagccagact gggaaaccgg aacattcaga
     2041 tttggaacgc tacatcttga aaattccgat ttttctgttg gtcgtaatgc aaatgtaatc
     2101 ggggacattc aggccagtaa atcaaacatt actattggtg acactacagc atatattgat
     2161 ttgcatgctg gtaaaaatat taccggtgat ggttttggct tccgccagaa tattgtgcgt
     2221 ggaaactcac aaggagaaac gctgtttaca ggagggatca cagcagaaga cagcactatc
     2281 gttattaaag ataaagcaaa agcattattt tcaaattatg tatacctgct gaacacaaaa
     2341 gcaaccatag agaacggtgc tgatgtgaca actcaaagtg gtatgttctc cacgagcgat
     2401 atcagcatct ctggtaatct gtccatgaca ggcaatcccg acaaagacaa taaattcgag
     2461 ccctcaatat atctgaatga tgcttcttat ctactgactg acgactccgc cagactcgtt
     2521 gccaaaaata aagcatctgt ggtgggagat atacactcca ctaaaagtgc atccatcatg
     2581 tttggtcatg atgaaagcga cctctcgcag ttgtctgaca gaacctcaaa agggcttgca
     2641 cttggtcttt taggtggctt tgatgtctca tatcgcggtt cagtcaatgc cccgtcagca
     2701 tctgccacta tgaacaacac ctggtggcaa ctaaccggag attctgcgct gaaaacactg
     2761 aaaagtacaa acagcatggt ctatttcact gacagcgcaa acaataagaa attccatacg
     2821 ctgacggtcg atgagctggc aaccagcaac agcgcctatg cgatgcgtac aaacctttct
     2881 gaatcagaca aactggaggt caaaaaacac ttgtctggtg agaacaatat tttactcgtt
     2941 gatttccttc agaaaccaac gcctgaaaaa caactgaata ttgaactggt aagcgcgcca
     3001 aaagacacca atgaaaatgt ctttaaagcc agtaaacaaa ccattggttt cagtgatgta
     3061 acgccggtca ttacaaccag ggaaaccgat gacaaaataa catggtcact gacaggctat
     3121 aacacggtag caaacaagga agcaacccgg aatgccgccg ccctgttctc tgttgactat
     3181 aaagcgtttc tgaacgaggt caacaacctg aacaaacgta tgggtgacct gcgtgatatc
     3241 aacggcgaag ccggtgcatg ggcacgcatc atgagcggta ccggctctgc cagtggtggt
     3301 ttcagtgaca actacacgca cgttcaggtc ggggtcgaca aaaaacacga gctggacgga
     3361 ctggatttgt ttaccggttt cactgtcaca cacactgaca gcagtgcctc cgccgatgtt
     3421 ttcagtggta aaacgaagtc tgtgggggct ggcctgtatg cttccgccat gtttgattcc
     3481 ggtgcctata tcgacctgat tggcaagtat gttcaccatg ataatgagta cactgcaacc
     3541 tttgccggac tcggaacccg tgattacagc acgcattcat ggtatgccgg tgcagaagcg
     3601 ggctaccgct atcatgtcac tgaggatgcc tggattgagc cacaggctga gctggtttac
     3661 ggttctgtat ccggtaaaca gtttgcatgg aaggaccagg gaatgcatct gtccatgaag
     3721 gacaaggact acaatccgct gattggccga acgggtgtgg atgtgggtaa atccttctct
     3781 ggtaaggact ggaaagtgac agcccgtgcc ggtctgggct accagttcga cctgctggct
     3841 aacggcgaaa ccgtattgcg ggatgcatct ggtgaaaaac gcatcaaagg tgaaaaggac
     3901 agccgtatgc tgatgtccgt tggcctgaat gcagaaatca gggataacgt ccgctttgga
     3961 ctggagtttg agaaatccgc ctttggtaag tacaacgttg ataatgctgt caacgctaat
     4021 ttccgttact cgttctgata ctccgtattc aaaagggcct gcgggccctt tacgctcagt
     4081 gcctgccgcc ctgatatgtc tgagagacat ttccgggatg tcagtcgact ggtttactta
     4141 actgcgatga ctaaagctgg cagtaatgcc ggctttttta cggccacata cgtcttcctc
     4201 tcatccggaa ctgtgataca aatcctgcat acaggattga ccgccggttc ctgcaaatgg
     4261 cacattttca gtaagatagc gaaggttaca tttgtcactg gttaacatac cga
//
LOCUS       DAZGXS010000226         4283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGXS010000226 DAZGXS010000000
VERSION     DAZGXS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            46..630
                     /locus_tag="ACHKG5_004633"
     CDS             46..630
                     /locus_tag="ACHKG5_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870580.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            710..1405
                     /locus_tag="ACHKG5_004634"
     CDS             710..1405
                     /locus_tag="ACHKG5_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0870581.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1434..3950
                     /locus_tag="ACHKG5_004635"
     CDS             1434..3950
                     /locus_tag="ACHKG5_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0870582.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3961..>4283
                     /locus_tag="ACHKG5_004636"
     CDS             3961..>4283
                     /locus_tag="ACHKG5_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870583.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         1233 a         1003 c          974 g         1073 t
ORIGIN      
        1 cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa taaagttaca
       61 aaaacagcta ttgcgggctt acttgcgctt ttcgcaggta atgccgctgc aaccgatggt
      121 gaaatagttt ttgatggcga gattttaaaa tccgcttgtg aaatcaatga ctctgataag
      181 aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt aggcgatcgt
      241 agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg gtgggagcac
      301 gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga taatggtacc
      361 gtccctaact tccctaactt ggcaaaagtg ggctcttttg ctggcacagc agcaacaggt
      421 gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactaaa tgctatgggc
      481 aatgataaca cagtttatca gatccctgcg gactccgctg gaattgtcaa tgttgacctc
      541 atcgcttact acgtatcaac cgttgaagca tccgaaatca ccccagggga agcagacgct
      601 gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat
      661 aacgcccttt tccttcctat ttctcgttgt atcaggttga aaaataacta tgtcaaaacg
      721 aacattcgcg atgatattta ccttgttgtg tagcttctgt atcggccagg cgcttgcagg
      781 gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt
      841 acctgtcgca aacaaaggcg cagaaacacc ttatttactg caatcatggg tagataatat
      901 agatggtaca agccgtgccc catttattat aaccccaccg ctaattcgtc ttgaagctgg
      961 cgatgattca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc
     1021 gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc
     1081 taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct
     1141 gaagggacgg gtaagcgatg cgtggaaatc actggaattt aaacgtagtg atcattcact
     1201 caatatatat aacccaactg aatattacgt cgtatttgcc ggactagcag tcgataaagc
     1261 cgatctcaca agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc
     1321 agcatctggc ggaaagaacg tgaaatgggc tgcgatcaat gattatggcg gcagttccgg
     1381 gacagaaact cgtccactgc aataacaaat ataaaaacac aggtcatcag ggaatgccac
     1441 aacgacacca ccagggacat aaacgcacac cgaaacagtt ggcgctcatt atcaaacgct
     1501 gtttgccgat ggtgctcact ggcagcggca tgctttgcac taccgctaac gccgaagagt
     1561 attatttcga ccccattatg ctggaaacca caaaaagtgg tatgcaaaca accgatctgt
     1621 cacgtttttc aaaaaaatac gcacaactac caggaactta tcaggttgat atctggctga
     1681 ataaaaagaa ggtttcacag aaaaaaatta catttaccgc caatgcagag caacttctgc
     1741 agccacagtt tacggtagaa caactacgtg agctgggtat taaggtggat gaaatcccgg
     1801 cgctggctga aaaagatgac gatagcgtga tcaactcgct tgaacaaatc attcccggta
     1861 cagctgctga atttgatttc aatcatcagc gacttaattt gagcattccc caaattgcac
     1921 tgtaccgtga tgcaagaggt tacgtctccc cttctcgttg ggacgatggt ataccaacgc
     1981 tgtttaccaa ctactcgttt acaggttctg ataaccgtta ccgccagggc aatcgtagcc
     2041 aacgacagta cctaaatatg caaaatggtg ccaattttgg cccctggcga ttacgtaact
     2101 attctacgtg gacacgcaac gatcaggcgt caagctggaa cactatcagt agttatttac
     2161 aacgtgatat caaggcgttg aagtctcagt tgcttctggg agaaagcgcc accagcggca
     2221 gtattttttc cagctacaac tttactggcg tgcaactcgc ttccgacgat aatatgttgc
     2281 caaacagcca gcgcggattt gccccaacgg tacgcggtat cgcaaacagt agtgcaatcg
     2341 tgactatcag gcaaaatggt tatgtgatct atcaaagcaa cgtgccagcg ggtgcctttg
     2401 aaattaacga tctctacccc tcttccaaca gcggcgattt agaagtcacg attgaagaaa
     2461 gtgacggtac gcaacgtcgc tttatccagc cttattcttc attacccatg atgcagcgac
     2521 ctgggcatct aaaatatagc gcgaccgctg gacgctatcg cgctgatgca aacagtgata
     2581 gcaaggaacc cgaatttgct gaagccacgg caatatatgg tttgaataat acttttacgc
     2641 tgtatggcgg cctgctcggt tctgaagatt attatgcgct ggggatcggt atcggcggca
     2701 cacttggcgc actgggcgcg ttgtcgatgg atatcaacag agctgacacc caattcgata
     2761 accagcactc ttttcatggc tatcaatggc gtacgcagta catcaaagat atcccggaaa
     2821 ccaacaccaa tatcgctgtc agctactatc gctataccaa cgatggctat tttagttttg
     2881 atgaagccaa tacccgcaat tgggactata acagtcgcca aaaaagtgaa attcaattca
     2941 acatcagcca gacaatattt gatggggtaa gtctgtatgc ctccggttca cagcaagact
     3001 attggggcaa taacgagaaa aacaggaata tctctgttgg ggtttccggc cagcaatggg
     3061 gaattggtta cagcctgaat tatcaataca gccgctacac tgatcaaaat aatgaccgcg
     3121 cactctcttt gaatctcagt attccgttag aacgctggtt accgcgtagc cgggtttcct
     3181 atcagatgac cagccagaaa gatcgcccaa cccaacatga aatgcgtctt gatggctcac
     3241 tgctggatga tggtcgcctg agctatagtc tggaacaaag tctggatgac gataacaacc
     3301 ataacagtag cgtgaacgcc agttaccgtt caccttatgg aaccttcagt gccggataca
     3361 gttacggtaa tgacagtagc caatacaatt acggcgttac cggcggcgtg gttatccatc
     3421 ctcatggtgt gacgctctcg caatatctgg gcaacgcttt tgcgcttatt gatgctaacg
     3481 gggcatctgg cgtgaggata caaaactatc cggggattgc tactgatccc tttggctatg
     3541 cagtggttcc ttatctcaca acttatcagg aaaaccgtct ctcggtagat actacgcagc
     3601 tgcccgataa cgtcgatctt gaacaaacaa cacagtttgt ggtgcccaac agaggtgcaa
     3661 tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt acttgttaca gtcagcgatc
     3721 gcaacggtaa accgttgccc tttggcgctc ttgccagcaa cgatgatacg gggcaacaaa
     3781 gtatcgtcga tgagggcggc atactatatc tctctgggat atcgagtaaa tcacaaagct
     3841 ggactgtacg ctggggaaat caggcagatc aacaatgtca gtttgctttt agtacaccgg
     3901 attcagaacc aacaacctct gtattacaag gcacagcgca gtgccattaa ggataaaaaa
     3961 atgaaaagag cgcctcttat aacaggactt ttgttgatat ccacatcctg cgcttatgcc
     4021 tcctcagaag ggtgtggagc tgacagcact agcggtgcga caaattacag cagtgtggtt
     4081 gatgatgtta cggtgaacca gacagataac gtgacaggac gggagtttac ctctgcaacg
     4141 ctaagtagca ctaactggca atacgcctgt tcctgctctg cgggtaaggc agttaaactt
     4201 gtctatatgg tcagccccgt acttaccacc actggacatc agacaggata ttacaaactc
     4261 aatgacagcc tggatattaa aac
//
LOCUS       DAZGXS010000227         4242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGXS010000227 DAZGXS010000000
VERSION     DAZGXS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            11..544
                     /locus_tag="ACHKG5_004637"
     CDS             11..544
                     /locus_tag="ACHKG5_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870584.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            594..1277
                     /locus_tag="ACHKG5_004638"
     CDS             594..1277
                     /locus_tag="ACHKG5_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0870585.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPKKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1290..3740
                     /locus_tag="ACHKG5_004639"
     CDS             1290..3740
                     /locus_tag="ACHKG5_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0870586.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQNNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3753..4242
                     /locus_tag="ACHKG5_004640"
                     /pseudo
     CDS             3753..4242
                     /locus_tag="ACHKG5_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT         1378 a          839 c          836 g         1189 t
ORIGIN      
        1 aggatgattt atgaaaaagt atattattcc cttcgttgcg acaacgttga tgtttagcgc
       61 aacaaccttc gccaaggatg tcgatcaagg aacactaacc attagaggag aagttgttga
      121 tacaacgtgt tactttgtta atggtgattc gacaacagat atcgttgtcg aagatgttgt
      181 aaccagtgca atgaatcaac ttatcaataa tgaaatctat actttaaaaa ccggttcaac
      241 ctctcatgac ttagaaattc agtgtgacgg aaatacagcc ccaagtatcg aagtactagc
      301 atcagagttt gatgctaata actttacgct gaataaagga actgctgaga acattggctt
      361 tgccattttc ctcgatgatg ggactttgac cagaatgaga ccagggttaa aaacaccgct
      421 gaaagataat ggctctggtc aatattttct caatttaaat gcacaatatg cccgcacaag
      481 tggcaatcct gttacaaaag gctccgtaga gtcaaccgta acgattaaaa tcagtgcaga
      541 ttaacgtttt ttgccggtag ctctgctacc ggtattattt acggagtgat tacgtgagaa
      601 taataacagc attactgatg tcatttttta ttttacctgt tcatgccggt gtggtcatct
      661 atggtacgcg cattatttat ccgaaaaaaa acagagaagt tctcgtacag ttaatgaatc
      721 aaagtaaaaa tgcctctttg attcaggcat ggatcgatga tgggaacacc actatcgcgc
      781 cagaaaaaat tcaggtcaca tttattctca caccgccagt aagtcgtgta gcaggtggtt
      841 ctggccaaca acttaaaata agaaaaatgc caaacaattt gcctcacaat aaagaaagtt
      901 tattttatct aaatgttctg gatatccctc caaacaatcc acaaaatgca gggaaaaaca
      961 aaattaaact tgcattgcaa aaccggataa agttactttg gcgtccaagc ggaatagctc
     1021 cggtagataa aaaaagcctt tctcaattaa atatcaaaaa gaaaaataac gccatctcaa
     1081 taaacaacga aacagccaac tggataacgg ttacaactat caaagcacaa aacgtgaaag
     1141 ttaataatga aagtatctta ctgcctccct tttcgcataa cgacattaca ttaaaaaata
     1201 accacgcatc agaatatgaa ctaacggtag ttgatgatta cgggaataac atccatagta
     1261 aaatcgccgc gagataacag gaaaaagtca tgttaaggac gacacgatgg gtcgctgcta
     1321 ttattttttt gtattctttt ccgggttatg ctgaagaaac ttttgatacg cactttatga
     1381 tcggcggcat gaaaggagaa aaagtttccg aatatcattt cgataataaa caaccgcttc
     1441 caggaaacta cgaacttgat ttttatgtaa ataaccagtg gcgtggaaaa caagatatca
     1501 cgatcccaga gtcaccagtc aaaccatgtc tgcctaaggt gctactaacc aaactggggg
     1561 taaagaccgg taacctcaat acagaagata attgtatttt actggataaa gcggttcatg
     1621 gtggacaata tcagtgggat atcagtgaac accgactaaa tttaacagta ccacaggcgt
     1681 atatcaatga acttgaaaga ggttacgttc ccccggaaag ttgggatcgc ggcattgatg
     1741 ctttttatac ctcctataat ttaagtcagt atcgctctta tgacagcaat aataacagta
     1801 atacagccag ttatggccga tttaacagtg gattaaattt atttagttgg cagttacact
     1861 ctgatgccag ctatagcaaa cccgatgata tgaaaggcac atggcaaagt aatactcttt
     1921 acctggaaca tggctggtcg cagatattaa gcacagttca gataggagaa aattacacat
     1981 cctcactcat attcgactct ctgagattta gcggaatacg cttatttcgc gatatgcaaa
     2041 tgctacctga ttctatgcag agctttacac ctttagtaca aggtgttgcg caaaataatg
     2101 ccttgataac ggtttcgcaa aatggctaca tcatttacca aaaagaggtt cccccgggac
     2161 catttactat cgcagatttg caactctcgg gcagcggctc tgatctcgat gtcagcataa
     2221 aggaagcaga tggcagtgtt cgttcctttt tggtgcccta ctcttccgtg cctaacatgc
     2281 tgcaacctgg tatttcaaat ttcgatttta tcgccggacg cagtaagata tatggtgtaa
     2341 agaatcagga ggactttctc gaggcaaatt atatatatgg cctcaacaat cttttgacac
     2401 tttatggcgg tacgatttta tccgataact ataatgctat aactctggga aatggttgga
     2461 atacaccgct gggtgccatc tcttttgacg ctacacgatc gagtagtaag ttaaataatg
     2521 atataacaca cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacgg
     2581 ccactcgatt tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg
     2641 accatcttta cgaaaatgat aaaataaatc atcagagtga ctatgacgat ttttatgata
     2701 ttggcagaaa aaatagcctt tctgccaata tcatgcaacc tttatccaat aatctgggaa
     2761 atgtatcatt aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt
     2821 accaattcag ttattccaat agctggcaac gcatcagtta tactttctct gccagccaat
     2881 cctatgatga aaacgacaaa gaagaggagc gttttaatct gtttatctcc attcctttct
     2941 actgggggga tgatattgcc aaaacacgtc accaaattaa cttatcgaat tcgacctcat
     3001 tttccaaaga tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg
     3061 atcagttaaa ttatggtata tatgttaatc agcaacaaca aaataatgat acatcgcttg
     3121 gtacgaattt aagctggaga actcccatcg ccacaataga tggcagctat agccattcta
     3181 aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta
     3241 ttaatatcac taaccagcta tccgatacat ttgcaattct ggatgcgcct gggttagaag
     3301 gcgcgcatat taatggacaa aaatacaacc gaacaaacag caaaggccag gttgtttacg
     3361 acctgatgat acctcatcgt gaaaaccatc tggtacttga tacagcaaac agtgaaagtg
     3421 aaacagaatt gcagggcaat cgtcaaatta ttgcgcctta ccgtggagca gtttcttatg
     3481 tgcagtttac aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt
     3541 cgccattaac ttttggctat gacgtactgg atctccagga aaacaatatt ggagtcgttg
     3601 gccagggtag tcgccttttt attcgcgtag atgaaattcc aactggcata aaagttgctc
     3661 tcaatgatga acagaattta ttctgtacta ttacttttca acacgttatc gatgaaaaca
     3721 aaacatatat atgccagtaa aaggagttta ctatgaagtg gcttttatta atcacattga
     3781 gtctatattc ttttattgtg caaagtgctc cctgtggatt gaccaatgtc ggggaacaac
     3841 gtggaacata tatactcaaa ccactgagta tgaaaggtaa tctcaccgcc gaaaaaattg
     3901 aaaatactgg gaatatcatt gagtgtgcag acttgcaaga gacgaccaac gccattaaag
     3961 ttcatttttt aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta
     4021 atttcccctt tgaatctgac ggtaaaaaaa atagcgcttc cagggaagtc atcttataat
     4081 ttagaagaaa tgctatctgc gataaatttc accattccat actctatcgc atctgtatca
     4141 gatagttcca acaccgcaga tatttttcca ggaactcctt ttcctttgtt gttcgaaatt
     4201 gaagtgagtt catgtacagg gaataatact aattgtaata gc
//
LOCUS       DAZGXS010000228         4214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGXS010000228 DAZGXS010000000
VERSION     DAZGXS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            93..824
                     /gene="artJ"
                     /locus_tag="ACHKG5_004641"
     CDS             93..824
                     /gene="artJ"
                     /locus_tag="ACHKG5_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0870587.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(875..2356)
                     /locus_tag="ACHKG5_004642"
     CDS             complement(875..2356)
                     /locus_tag="ACHKG5_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP0870588.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2368..2688)
                     /locus_tag="ACHKG5_004643"
     CDS             complement(2368..2688)
                     /locus_tag="ACHKG5_004643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0870589.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(2710..4053)
                     /locus_tag="ACHKG5_004644"
     CDS             complement(2710..4053)
                     /locus_tag="ACHKG5_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP0870590.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
BASE COUNT         1211 a         1083 c          891 g         1029 t
ORIGIN      
        1 tatttattgc atataaattc acttgatggc attgttatcc catgccgcag acacggccaa
       61 taatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt gccgcattac
      121 tggcatcctg tgcgttcggc gcttctgccg cagagaaaat caattttggc gtatcagcga
      181 cctatccgcc gttcgagtcg atggatgcca ataaccagat cgtcggcttt gatatcgacc
      241 tagcaaaagc gttatgcaaa cagatgcagg cagaatgtac ttttaccaat cacgcgttcg
      301 acagcctgat cccgtcactg aaattcagga aatatgacgc cgtaatctcc ggtatggata
      361 tcacaccaga tcgtagcaag caggtatcgt tcaccacgcc ctattacgaa aactcagccg
      421 tcgtgattgc caaaaaagat acctacaaaa cgtttgccga cctgaaaggc aaacgcattg
      481 ggatggaaaa cggtactacg caccagaaat atattcagga tcagcacccg gaagtgaaaa
      541 cggtctccta tgacagttat caaaatgcct ttatcgatct gaaaaatagc cgtattgatg
      601 gcgtatttgg tgacactgcg gtggttaatg aatggctgaa aaccaatccg caactgggcg
      661 ttgctactga aaaagtgacc gatccgcaat actttggcac cggtctgggt atcgctgtac
      721 gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca attaaagctg
      781 acggtacata tcaacaaatc agcgatcagt ggttcccgca ataattattc atttgccgga
      841 gtgggcgaac acccactccg gcaaaatcac ttctctacgc taatcccagg cgctgaaact
      901 gctctggcgg cgcatcatta tctttcatca actgaatcag caacgcgatc atccgggact
      961 cgatcacatc atcatgaatc ggatgtagct gctgcggatc gttttgcaga tcaaacagca
     1021 aatgaccaaa ctgattcaac cccttatatt tttcctgtgc cgggatgcgt aatacctgta
     1081 accctttggt aaaacgaaac ggtggggcca gcgataaagc ccgcatctca tccaccggat
     1141 agcggctgtt cattttgcag gacatcaacg tgtagttggc aatatcatcc tcacgccccg
     1201 gcagcgctgc acgcatatac acataacgcc catcagttac gttagcatga ccactgaaaa
     1261 caccaaacat cgccgagctg cgtactggtt catcgtgact gaccgttttc gccagcggtt
     1321 gcccctgcac atcgggcggc actggctgtt gaaaaaagtc atacaacgtt ggcgcccagt
     1381 cgatcatttg taccagtgcc tgtcgccgct ctccttttac accactgcgc gggttccaga
     1441 taaacaacgg attattggca acttcgttgt aataaggcat ctggtttttc gcccaccagc
     1501 catgctcacc gagtaaaaag ccgtggtcag tgccgacaat cagcatggta tctcgccaca
     1561 gatcgtgctc atccatcagg tcgagaatgc gccccaggtt gcgatcgcac atcgacacca
     1621 gcgaacgata ccggcaacgg atgtgctcca cggcctcgtc gctttcctgc acccggccac
     1681 gcggccagtc ataatgcggt ccatcccagt tatcttcata caaggaaaga tactcttccg
     1741 ttgtgtgaaa cggctcgtgc ggatcaaacg tttcgatctg caataaccag ttatcttccg
     1801 cgtgattttt gtggataaag gcacagcctg catcaaacac tttagtttgt ggaaaatccg
     1861 cttcctgctg aatatattcc cggtttgccc agtcatggcg ccacagacca gaaacaccgc
     1921 caccggtctg cttttgcggc acccgtagca cctcgggaat aggcggttgc gcaacgcttg
     1981 catgccagtg atcgccttcc tgtccgcgca caatctccca cgaactgtag cgattatgat
     2041 aattcccgcc gccatcttcc cagtaatgca aatgatcgct tatcaggtgg gtatagatcc
     2101 ccgccttttt aagcagctcc ggcatggaat cgtcaaacgg ttctaacggc ccccactccc
     2161 gatgcaggaa gttacatcgc ccggtatgca actcccgccg ggcaggcata cacggcatac
     2221 tgccgacgta actgttttca aaggtggcgg catgagccgc cagacgttgg aaattaggcg
     2281 ctttggttaa cgcatcgcca taaggtggca gataacgttt attcagacta tcgaacagca
     2341 gaataatcgc tctcattacg ccctacctta ttgattaatt aaatgcggaa aatgttgctg
     2401 aaccacttca taaatttgct gaccaatagt ttcaccgtcc ataatattct ctatcggaat
     2461 caaatattgc ccttgtttcg ggtcaagact aaccacatcg agtaattttt tggaaatagc
     2521 aaaaatatcc gcccagtcgg taaatccgcc gacttcacct gcggcggtat gttccatttc
     2581 aactttgaaa tcatatttgg tcagcgcttt tttaacgacc gtttgtaaca taacgctact
     2641 gcctacaccg taaccacaga caaacagaat attaattgta tctttcattt ttaaaccctt
     2701 aatatttaat taacccgcgt aaggaccaag atggaatgtt gcagcgataa atttcagaac
     2761 ttcacaaata gcaggccata cggttgccca gtcaaatatt cctgtccagc cgacgccatt
     2821 ttcagcaagc ccggttaacg gaatagccca caccgtaccg aaggtctgaa taatacccag
     2881 caagaacgta cagataatta cggaacggta tccgcccatg cggtttgcta atacgccaat
     2941 tggaccgcca gagaaaaaca gaggtacaaa gccaggcaat accataattg ggctgtgaac
     3001 cagtaataag atccccatcg aaaaaatggt gccaaaagta cagaagacga agcctaacgt
     3061 tgcggcattg ggtgaaaaag gtaacagcgc tgcaacatcg acagcaggaa tagcgttagg
     3121 aataaagcga tcttgccagc ctttaaacga accgttaatt tcgcccacca tcatgcgcac
     3181 accctgcagc agaatgacca tatacatgga gaactgtaag ccaaggttga tgatatagat
     3241 gtaccagggc ttgcctttcg ccattaactg gacattatcg atacccgttg atagcaagaa
     3301 accaccgaca aacagcgtca tcacaatggc gatagccgtt acgttatggt taaaaatcgc
     3361 cagccacccg ggaagtttca ggttttcagc atcgtgcttt tcagggtcac caaatttatg
     3421 ggcgaatttg gagaaaaacc acaatcccaa catctggtta tgagcaatgg taaaaccggc
     3481 atctttggta actttggcaa tgggcttaac aatgagcgtg gtggagaacg cccagaatac
     3541 acccgttaat attccggcaa taataatgga tgtagtccag ctaaaaccca gccaaaagac
     3601 aatcaaccat aataccgcct gagaatgtga aacaccggta ttccctgtca aaaatatccc
     3661 tttggcacca gtataccgac caaataatac cagcagcagg ttggtaaaaa acgccagtac
     3721 aaatacatac ccgacataac caaaattatc ccccaatgca atttgcgttg ccgctttcat
     3781 tgcgtaggaa tccattaaat atccgtgaac accataggct ttacttacgg cttctgctac
     3841 cggtttaaat gttgcggaaa actggaaacc accaaataac accatcatta atcccaccat
     3901 ggcgctgacc atgccggtaa taatttttat tggcgatttc ttatcaaggg cataaccaat
     3961 acccaccagc aaaccaatta aaattgccgg ttcagttaaa aacgtttgaa taacatctat
     4021 gaatttggca aacatcgtcg gaacgccctc catacgcgta catttccgtt gtcaatgcat
     4081 gaaccttctg ctgacgttaa taatgtctca ggaaattttg tgtttcgcta agcaatgcct
     4141 ctgtatgtat tcggtaagtg cactgtagta atcaaaacag ttcaattaaa gatcttattt
     4201 gattatttgt gatt
//
LOCUS       DAZGXS010000229         4204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGXS010000229 DAZGXS010000000
VERSION     DAZGXS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            294..2444
                     /gene="btsT"
                     /locus_tag="ACHKG5_004645"
     CDS             294..2444
                     /gene="btsT"
                     /locus_tag="ACHKG5_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0870591.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2494..2697
                     /locus_tag="ACHKG5_004646"
     CDS             2494..2697
                     /locus_tag="ACHKG5_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0870592.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2708..3664
                     /gene="yjiA"
                     /locus_tag="ACHKG5_004647"
     CDS             2708..3664
                     /gene="yjiA"
                     /locus_tag="ACHKG5_004647"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0870593.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLCERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3733..>4204)
                     /locus_tag="ACHKG5_004648"
     CDS             complement(3733..>4204)
                     /locus_tag="ACHKG5_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFP0870594.1"
                     /translation="INQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQ
                     DESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQ
                     HYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT          952 a         1096 c         1072 g         1084 t
ORIGIN      
        1 atatttatag tatgaatata atttttaaat gagttacaca accgagggct aaaaatcgct
       61 tcggttgtga tcatcatcac ataaataacc ttcataaacc ttgctcaaaa ccgctcaacc
      121 gcaaaactga ccgcttacgt ccctaaaata accactcagt tatttacctt actttacgcg
      181 cgcgtaactc tggcagcatc attgcaggat agcggtcaat ttacctcctc aaacgcaacg
      241 caaacctaga acggcttcgg ccaactatta atcaatacat gccaggtttt actatggata
      301 cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt gcattctgcc
      361 tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc gtggtcgcct
      421 ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtacatcgcc cagaaggtga
      481 tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt ctgaactacg
      541 ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc ggtgctggtc
      601 cgctggtggg accggttctc gccgcgcaga tgggctacct gcctggcacg ctgtggctgc
      661 tggcgggggt agtgctggcc ggtgcggttc aggactttat ggtgctgttt atctcctctc
      721 gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatggggcca gtaccgggga
      781 ctatcgcgct gtttggctgt ttcttaatca tgatcatcat cctcgccgtc ctggcgctga
      841 ttgtggttaa agccctggca gaaagtccgt ggggtgtctt caccgtttgc tcaaccgtac
      901 caattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt gtgggtgaag
      961 tctctgttat tggtatcgtg ctgctggttg cctctatcta cttcggtggc gtgattgctc
     1021 acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc ttcgcgctga
     1081 ttggctatgc gtttgtttcc gcgctgctgc cggtgtggct gatcctcgca ccacgcgact
     1141 atctggcaac cttcctgaag atcggcgtta tcgtcggcct ggcgctgggt atcgtggtgc
     1201 tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact ggcccgctgt
     1261 ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct
     1321 tccacgcgct gatctcttcc ggtacgacgc cgaaactgct ggctaacgaa accgacgcgc
     1381 gtttcatcgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg gcgctggttg
     1441 ctgcatccat catcgaaccg ggtctttact tcgcgatgaa caccccgccc gcgggccttg
     1501 gcatcaccat gcctaacctg catgaaatgg gtggcgagaa cgcgccgatc atcatggcgc
     1561 agctgaaaga cgttaccgca cacgcggcag cgaccgtcag ctcttggggc ttcgtgattt
     1621 cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc ctgaaccgtg
     1681 caggtggtgc gccgacgctg gcggtaggta tcgctcatgt gttccacaaa gtgctgccga
     1741 tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg ttcatcctga
     1801 ccgcgctgga tgcgggtacc cgttctggcc gctttatgct gcaagacctg ctgggtaact
     1861 tcatcccgtt cctgaaaaaa accgactctc tggttgctgg tatcatcggt actgcgggct
     1921 gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta
     1981 agagcctgtg gccgctgttc ggtatctcca accagatgct ggcagccgta gcgctggtac
     2041 tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta actgttgttc
     2101 cggctgtatg gctgcttatc tgcaccacct gggcgctggg tctgaaactg ttcagcacca
     2161 acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag attgctaacg
     2221 gtactgacct tacggcgcag cagattgcca acatgaacca catcgttgtg aacaactaca
     2281 ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc ttctacggtt
     2341 tcaaaacctg gcttgcggta cgtaacagcg acaaacgtac tgacaaagaa acaccgtacg
     2401 ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccgtgt ttagccccgc
     2461 ttcggcgggg ctttgttctt atcagagtga actatgtttg gtaacttagg acaggcaaaa
     2521 aaatatctcg gccaggcggc gaagatgttg attggcattc cagactacga caactacgtc
     2581 gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga attcttccgc
     2641 gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg
     2701 agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg cgggcaaaac
     2761 caccctgctg cgccatattc ttaacgaaca acacggctac aagattgccg tgattgaaaa
     2821 cgaatttggc gaagtctccg ttgatgatca actgattggc gaccgcgcca cgcagatcaa
     2881 aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggaag acgcactact
     2941 cgacctgctg gataatctcg ataagggcaa tattcagttc gatcgtctgg tcattgaatg
     3001 caccggcatg gccgatcccg gcccgattat tcagaccttt ttctcccatg agattttatg
     3061 ccagcgttac ctgctggacg gcgtgattgc gctggtggat gcggtacatg ccgatgagca
     3121 gatgaaccag ttcaccatcg cccagtccca ggttggctac gccgaccgta ttctgctgac
     3181 caaaaccgac gtcgcaggcg aagcagaaaa actgtgcgaa cgcctggcgc gcatcaacgc
     3241 ccgtgcaccg gtctacaccg ttacccacgg cgacatcgac ctgggcctgc tgttcaacac
     3301 caacggtttt atgctggaag aaaacgtcgt cagcaccaaa ccgcgtttcc actttatcgc
     3361 ggataaacaa aacgatattt cgtcgattgt ggtagaactg gattacccgg tagatatcag
     3421 cgaagtttcc cgcgtgatgg aaaacctgct gctggaatcg gcggataaac tgctgcgtta
     3481 caaagggatg ctgtggattg acggcgaacc taaccgcctg ctgttccagg gcgtccagcg
     3541 cctctacagc gccgactggg accgcccgtg gggcgatgaa aaaccgcata gcacaatggt
     3601 ttttatcggg attcagttgc cggaggaaga gattcgggca gcgtttgcgg ggttgaggaa
     3661 gtaagttcgg gaaagatcaa ctttttgaga gttccaccgc aacattatta cgagtggaac
     3721 tctttagata tgtcagacca accaaagctt cttcaattgc gttttcattt tttcatgtct
     3781 cattttcagt tcagccaatc cccattctga agaattacaa acatcttttg ttaaatcaaa
     3841 agagctttta ctattaagtt tctcataaat agatttcttt ttgataaaat catagtgttg
     3901 tgcttcagaa tttttggaac cacttatgag tgttaaatta cctattttat gcagccagta
     3961 gacatgctct tgaggttgga atctattaat ccagtattca ttaactaatg cttgaggaag
     4021 aatatgctca attgtgattc tccccgtata ggttttaatc acgctctcat cttgttgctc
     4081 catatctaag cgaagcaaaa ttgcttttat taagttcact tgattcggac gaggctcata
     4141 taaatcttca tccagagcag ctataaaacc tgaattatct gcatgttggt ttatctgatt
     4201 tatc
//
LOCUS       DAZGXS010000230         4170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGXS010000230 DAZGXS010000000
VERSION     DAZGXS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..275)
                     /locus_tag="ACHKG5_004649"
     CDS             complement(<1..275)
                     /locus_tag="ACHKG5_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFP0870595.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIES"
     gene            complement(286..2766)
                     /locus_tag="ACHKG5_004650"
     CDS             complement(286..2766)
                     /locus_tag="ACHKG5_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0870596.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            complement(2782..3456)
                     /locus_tag="ACHKG5_004651"
     CDS             complement(2782..3456)
                     /locus_tag="ACHKG5_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0870597.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSVKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            complement(3544..4086)
                     /locus_tag="ACHKG5_004652"
     CDS             complement(3544..4086)
                     /locus_tag="ACHKG5_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870598.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT         1190 a          992 c          751 g         1237 t
ORIGIN      
        1 aaagactcta ttttaacctt taatttttcg ccattaccaa aaggcccaac ttcaacccaa
       61 tcactttgga tatttttata aactatttta tccgtgctcg tatcacaaga tatcccagta
      121 aacacgacac ttttactaaa ggaagtttca ttcgctaaaa tctccccaac attaatacca
      181 tctgtaatat ttaatgatgc agtagtacga cacccagccc aggtaaaatt agcccacaat
      241 ataaatatta gtaatattct atttctgaac atcatatttc acctctcacc gacagatata
      301 atttttactt tcatcaatct ctttaccgaa ggtgattgtg catgaaagcc cttgttgctt
      361 atcaattgcc acattaaccg ctggcggtat ttcattggtg cgaataaata tctgactgcc
      421 ctggccgaca acaccaatgt tatgaccatg catatcattg acttcataac caaacgttaa
      481 tggctgtcca tcggatctta atgcttttat aaaccagggc ttacgctgat cggtatcaaa
      541 atcaaccaga acgaccgcgc cgcgataagg ggcggtcatt ttacggttcc cgcgtaattc
      601 tgtttcgcta tcgctttgcg acacatccat catcaggtga ttttcccgat aaggtgtcag
      661 tccgtcgtac accaccacac cattacagtt tgttgtacga tatttttgcc cattgacata
      721 agcatctttg attcccggcg catgcatcac agcaaacgtt tcggaaagac ggttcgccag
      781 attaacgcca ccagaccagg caaccagtcc ccctgaaaca ctggctccga cctgtcgata
      841 agtactcgac tgactatagc tgccattcac tgttgcggcc ggtgcggtcc aggtcaaatt
      901 ggccccagct gtcgtttcat ttccctgatg ttgatggctc aagttgatac catagttaaa
      961 ctgatcgcgg ttcccaacgg ttcccgataa tccggtattg tttgaagcaa aaccctgatc
     1021 atcaaaggtc gttgagttag acatatatat ttgccgtcgc ggcgtcgtaa cgtcatcgcc
     1081 ccagtcaaag ggaatcgata taaaaatatt aaagcgtttc tcttcggcgt ggttctcgtc
     1141 atacgcctgg cttgccgcga gggtatagct tatccgccgc cagttattgg aataactcag
     1201 ttgataatct ttactactgc cgcttcgccc ccagtaatca cgccataaag tacttaatga
     1261 cacagatccc cagccttctg gcaatgactg actcatattg gcggaaaagc tatttttgcg
     1321 accaaaatca ttctggtaat aatcggcaat atcatagaca tcgtttttat cacgacgata
     1381 attatcttta ttatttgccc acacataatc gttaaatgtg cggtaatcac gcgacgaata
     1441 acgccaggcc gccagaccaa aacgtgttga tgtttggctc aaaaatttgt tataggcaat
     1501 ttgataactt tgtccgtcaa acacatcacc attgtcttgc ttactgtgcg atttagttgc
     1561 atcgactgaa attgcgccaa tacgcgtgtt ccaacctgtt ccgagggtaa aggcatagta
     1621 attattagca accatcgtgc cgccatacag cgtcaataaa ttattaaaac catactgata
     1681 acccgcctgg acaaaatcac tttgcttgct cgcaccttca atatgactac ggcctgccgc
     1741 aaaatcatat tttgatacac caggttgcag catattaggt actgccgcat aaggcaccag
     1801 atacgtagtc acagaaccat cggcttcctt aacactaaca tcaagatccg cccctccgcc
     1861 cgctaactgc aaatcactaa tcgaaaacgg acctggtgga acctctttct gataaacaac
     1921 aaaaccgttc tgttcaatag ttaccaacgc gttactctgg gcaatcccct gtacccgtgg
     1981 ggtaaaattt tgcttcgagt ttggcaacat ctgcatatca cgaaataacc gaaccccggt
     2041 gaagcgaacg gaatcaaata tatcggctga ggtgtacata tcaccaatac gcagcgtacc
     2101 gagaatttga gaaaatccat gctccagata cagcgtgttg cttttccact caccaggatt
     2161 attatccgtt ttgctaaaac tggcatctga atgcagctgc catcccagca aatttagccc
     2221 actgttaaaa cgcacataag tgctcttact attgcccgag gctttatagt cactgtaata
     2281 ctgacttacg taataagacg tataaaacgc attgattccc cgctcccagt tttctggcgg
     2341 aacgtaacca ttttccagct cttcaaccca ggcttgcgga acagcgagat ccaaacgaaa
     2401 aatcccaata tcccaggagt agcttccgcc ctgaacaagt tgctcaaatg ttaaacactg
     2461 attttcgcgt gcaaaattat cgctatttat acctaaccgc tttacaattt cacgcgtcag
     2521 gcaagtttca tgtgggttat ctttgacgat aatctcatat tttccacgcc attgtttatt
     2581 gacataaata tcaatgtcgt actgcccagg taaaggctga ttatcatcaa gacgcaaatt
     2641 tgttacttgc tcacctttca ttcctcccat cataaaatgg gtgtcaaagg tttcttctgc
     2701 tgcatgtgct tcaatgccga gcaataacgc gactattgct gatgcaagcg gggtcattct
     2761 caacataggc agctcctgca attaaatttt gtcactaata tagttgccgt gatcatcgat
     2821 aatggtcagt tgccagccat tcgcattatt attttttaca ctaacattct gactttcttg
     2881 tggtgcaatc atgattgttt cataattcac tttgacgtta ttagccttaa catcagaaat
     2941 cgttacccaa tttggcgagt cattttttat gactacgtta ttaccgctac gactcactcg
     3001 taatttttta aaagtcgttt tatttaccgg agcaatacca gcaggtcggt aaaacaactt
     3061 gattctgttt tgcatggcaa attttaacgc attctttcct tcatcttctg ggctattcgg
     3121 tggaatatcc agaacattaa gataaaaaat actttcctta ttactgggta atttattagg
     3181 catgatttta atttttattt gttgcccaga attggccgct actttcacta ctggtggcgt
     3241 taacatgaaa ggaacctgaa tcttttctgg cggcagagac gtgtcgccat catcaatcca
     3301 cgcctgcatc agcgaagagc ggtttccctg attcatcagt tgtaccatga cctctttctg
     3361 ctctgccggg taaatgatac gggtcccgaa aataaccatt ccggcatgca cggggaatat
     3421 catagaagag atgagtaaag ataataaccc tttcatctta atgcctctta tatttatgct
     3481 ggccgccatg aatatggcgg ccattttaac ttactgaaaa aacaagatga ttattttaaa
     3541 tatttaatcc tgggcaacac gcataaccaa tgaagagtta actgtaccag atgcaacacc
     3601 actgtcagtc aacttcagat attttgcctt aaacggtatt gcatattgtc catcagcagt
     3661 tttataactt tcaatattaa atggtgcgga aatatccagt acgtcactgc tatctcccaa
     3721 atagagatag tatccaactg tgtcattact actggatatt gccttatcat taccaagaat
     3781 tttctcgttg gtgatcaacg aaagttttac gccatcaata gtcttcggac aagtaatttt
     3841 cagcatactg ctggttacac caaccgcgcc atcataagtt gaatatttcg ctttaccatc
     3901 aaatacagtg gtgggtacag tcggcatatt aatactgact gaatcaccgc cagcgacaaa
     3961 tgtacatggt gattctgcaa tattaccttt aatggttaaa gttccggtat ctacttcagc
     4021 agcgaaaata ccagcactca taaatacaga agataagaca gcgacagcaa taattgaatg
     4081 tttcatgatg cattccttaa taagtttatt ttgttttgtt aagagtctgt atctcttgga
     4141 aacaaatttc tcacatcaaa aaggttgccg
//
LOCUS       DAZGXS010000231         4141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGXS010000231 DAZGXS010000000
VERSION     DAZGXS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            31..390
                     /locus_tag="ACHKG5_004653"
     CDS             31..390
                     /locus_tag="ACHKG5_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0870599.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHKG5_004654"
     CDS             399..929
                     /locus_tag="ACHKG5_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0870600.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHKG5_004655"
     CDS             1171..1368
                     /locus_tag="ACHKG5_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0870601.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2583
                     /locus_tag="ACHKG5_004656"
     CDS             1519..2583
                     /locus_tag="ACHKG5_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0870602.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVPGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSGSTVKAAMALGRRATGVEL
                     ETERFEQTVREVQDLIIRNG"
     gene            2624..2699
                     /locus_tag="ACHKG5_004657"
     tRNA            2624..2699
                     /locus_tag="ACHKG5_004657"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2657..2659,aa:Ile,seq:cat)
     gene            2780..2856
                     /locus_tag="ACHKG5_004658"
     tRNA            2780..2856
                     /locus_tag="ACHKG5_004658"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2814..2816,aa:Arg,seq:tct)
     gene            3380..>4141
                     /locus_tag="ACHKG5_004659"
     CDS             3380..>4141
                     /locus_tag="ACHKG5_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0870603.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QVITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAML"
BASE COUNT         1038 a          925 c         1200 g          978 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcccgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccaaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcggggtcga cagtgaaagc agcgatggcg ctgggacgtc gtgcaactgg cgttgaactg
     2521 gagactgaac gttttgagca gacggtgcgg gaagtacagg atttaatcat tcgtaacgga
     2581 tgagattgcg gagttaatca tgcgtcgtta ttattcagca atcggccctt tagctcagcg
     2641 gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa gggccaccaa
     2701 ccgccactag ctcatcagga aagaacgtca ccctgtgcga gattcggagt ccccggtggc
     2761 ggtccattat cggtattctg cgttgttagc tcagccggac agagcaattg ccttctaagc
     2821 aatcggtcac tggttcgaat ccagtacaac gcaccacacc acacttatct gccctgactc
     2881 tcttttgcgg gctttttatt acaggaaaga caccggacag tgaaatgtta aatgcctcac
     2941 aattcaggca gttgactgtt gcctgacatg ctgagcgttt gttaaaaaaa tcctgcatga
     3001 tgaatccccc tgagcggcgg ggcataatga cagatgtttg gttgcgtatt gtataggcaa
     3061 gttgcggatt ctgtctggtc attgcagaat tcaccgggag gcacccggca tcatgctgta
     3121 tacagagatt aggcatatat ccaggcttct catcgcagga gcctttttac atgcaaaaaa
     3181 aagcccgagt gggttcgggc aacagcatga gatacttgca ttgtcatttt tatcgtgtgg
     3241 attttaacca gggtttataa ggctgcgcaa ctgcgcggcc tttttcgttt tgcgggctgc
     3301 ggttctcctc ttttgattct ccttgtggcc ggaccgtggc ccgcaactgt tgaggaaaat
     3361 cccggaaagg ggaggaataa tgacatttaa acattatgat gttgtcaggg cggcgtcgcc
     3421 gtcagacctt gcggaaaagc tgacacacaa actgaaagag ggctggcagc cgtttggtag
     3481 tccggtggcc ataacccctt ataccctgat gcaggtgatt acagcagaag gtgatgtggt
     3541 ggtcagtggt gcaactgagc cggattggta ctacgtcatc gtactggccg ggcagtccaa
     3601 tgccatggct tacggtgaag ggcttccgct gccggattca tacgatgctc cggatccgcg
     3661 cattaaacag ctggcgcgcc gcagtacagt tacgccgggt ggggctgcct gcagatataa
     3721 cgatattatt ccggccgacc actgcctgca tgatgtgcag gatatgagta cgctgaatca
     3781 tccgaaggca gacctgagca aagggcagta cggctgtgtc ggccagggct tacatattgc
     3841 caaaaaactg cttccgtata tcccgaataa cgcggggatc ctgctggtac catgctgtcg
     3901 tggtggttcg gcattcaccc agggcgcgga ggggacattc agtgcggacg cgggggccag
     3961 ccaggattcg gcgcgctggg gtgtgggtaa accgttatat caggacctga ttgcgcgcac
     4021 taaagctgca ttacagaaga acccgaaaaa tgtgttgctg gcggtgtgct ggatgcaggg
     4081 agagtttgac atgagcgccg ccacccacgc acagcaacct gcgctgttta cagccatgct
     4141 g
//
LOCUS       DAZGXS010000232         4136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGXS010000232 DAZGXS010000000
VERSION     DAZGXS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(119..787)
                     /gene="artM"
                     /locus_tag="ACHKG5_004660"
     CDS             complement(119..787)
                     /gene="artM"
                     /locus_tag="ACHKG5_004660"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0870604.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(787..1503)
                     /gene="artQ"
                     /locus_tag="ACHKG5_004661"
     CDS             complement(787..1503)
                     /gene="artQ"
                     /locus_tag="ACHKG5_004661"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0870605.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1510..2241)
                     /gene="artI"
                     /locus_tag="ACHKG5_004662"
     CDS             complement(1510..2241)
                     /gene="artI"
                     /locus_tag="ACHKG5_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0870606.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2259..2987)
                     /gene="artP"
                     /locus_tag="ACHKG5_004663"
     CDS             complement(2259..2987)
                     /gene="artP"
                     /locus_tag="ACHKG5_004663"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0870607.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3205..3720)
                     /locus_tag="ACHKG5_004664"
     CDS             complement(3205..3720)
                     /locus_tag="ACHKG5_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0870608.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1058 a         1067 c         1026 g          985 t
ORIGIN      
        1 atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct gatgtctgat
       61 gcgacgctgg cgcgtcttat cagacctaca aaacccccgg cgaatgtacg cagccacatt
      121 aattccttcg ctcaaacgcc agcgctttgc gctcgatcag acgcatcatc agcgtcagca
      181 ggccgttaac gaccagataa ataatccctg ccgcgccgaa caccatgaca tcgtaggtgc
      241 gtccgtacaa caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag
      301 aggtactttt gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg
      361 catacggcag caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact
      421 gccactgacc ttccgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg
      481 cactgttcag cgacaacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca
      541 gtgccggata ctcctgcaac gtcgggaact gacccggccc gtaataaatc aggaagatct
      601 gcaccagcag cggtgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca
      661 ccggcgtttt cagcgtcagg atgatggtaa aaatcaacgc cagaatcagt gccacaatca
      721 gcgaggcaac ggttagcgtc aggctggtgt gcagcccttt catcagttcg ggtaaatact
      781 caaacattag ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat
      841 gtactgactg agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa
      901 tggttcctgg gtacgagtag cgatgctttt ggtttgcagc attaaatcat tcacactaat
      961 caaactgacc agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag
     1021 cgcatgacgc cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc
     1081 cagcgcctgg ccggattccc actgacccat cggcaccgct ttcaacgcgc cgcgcagcgt
     1141 ttgcgaggca taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacgggct
     1201 aacgtcgaag ttttcaatct gcatctgcac cgggatctct acaatcccaa gattgatagt
     1261 gaagccatcc gaaagtgtca gcagcagctg cgaggagcca aaatagataa acagcaccac
     1321 cagaatttct ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg
     1381 acgccatttc gcagactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa
     1441 tgcacaaacg gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt
     1501 catcaggaat tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt
     1561 cactttttcc agcgcagtgt tgagtttctg ctgcagctca gtgttgccct gacgtaccgc
     1621 gatgccgagg ccagtaccga agtaatcttt atcggtcact ttatcgccca ctgccgccag
     1681 tttcgggtta tctttcagcc attcagttac cactgcggtg tcaccaaata cgctgtcgat
     1741 acgaccgttt tgcagatcca gttttgcgtt ctggtagctg tcatacggca cggtggtgat
     1801 ttccgggtgc ttatccataa tgaatttctg gtgagtcgta ccattctgta cgccgacttt
     1861 tttgcctttc agctgatcaa cgctggtgta tttaccctgc tgacccacaa acagggcaga
     1921 gttgtcatag tacggcgtgg taaacagtac ctgcttttca cgttccgcgg tgatatccat
     1981 cccggccatc accgcttcca cgcgacggaa tttcaggctt gggatcaggc tgtcaaatgc
     2041 ctggttagag aaagtacagg tcgcatcaat ctctttacac agcgcctgtg ccaggtcgac
     2101 gtcaaaacca acgatctggt tgtttgcatc aatcgattca aacggaggat aggaggcttc
     2161 ggtagcaaaa cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc
     2221 ggcaatcaga acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta
     2281 aatgcttcgg tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgaccg
     2341 ttttccatat acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg
     2401 atcacctggg taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta
     2461 atttccgggt ccagtgcggc ggtcggttca tcaaacagca gtacctgcgg ttccatcatt
     2521 aacgcacggg caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga
     2581 tcgctataag gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga
     2641 tctttactca accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga
     2701 tgcggccaca ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc
     2761 gctttgtctg agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg
     2821 gagcgcggca tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg
     2881 gggccaagta acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc
     2941 tgatgcgcgc cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata
     3001 ctggcagtct gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat
     3061 atttctgctt taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac
     3121 gggggcggtg gtcggcgagc ataaatgtcg gcattcctca cgaaatgccg gacaatttac
     3181 ggggtttatt ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc
     3241 cgtggcatga acgctaccgc ctaagtagcg cacatcgtcg atcacccaac actggccttc
     3301 ctgaatcatt aacacttcat cctgccagcc ctggtcaccc tgcttgagat ccacgcgcaa
     3361 tggaatgtta cgggcatcac gattagggat agtcgatgca ctggcaacgt gggcgctgtc
     3421 tggcaaggtg gttcgactgg agaacggatc actgcttaac aattcacgat gactattatc
     3481 gcggctggca tcgctaagta gggttgccag tttgtcactc aaataggggc gcagggcggt
     3541 aatgtcattg ctgcgatgtt gaatgtggta gtcatagaat tgttgggcca cgctgtcagg
     3601 ccccccctgg acacaggaac cggtgcgggg accattatct ttataagctg gcgtgacggt
     3661 ggtacaggca ctcagcagca gtgcacatgg aataagtaga gatagtgaac ggtagcgcat
     3721 aatcatttcc ttattagctt gctgtctgcg tcacactacg accaaacgct atcagattat
     3781 ttaatacatt gtttttaata caattctgaa acaatctgaa cacctgattg ggtttgagtc
     3841 cattgctggc gacccactca aggttgctcg tagctgactt acttaaatta cccaaacaat
     3901 agacagtgta aaccatttgt gaggttgagc gtgttatatg gctgaaatga tcatttaagg
     3961 taaatatgat agttctgaaa tggtggtata aatcctcctg tagaggttaa gagcatctgc
     4021 aagaagccag tttcacattt ataacgcgtg ataaatgaac gcggttattg tctaatcatt
     4081 acaaattttt cacatgcgat aagagatcct gctattaaga tgattgccaa aattac
//
LOCUS       DAZGXS010000233         4134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGXS010000233 DAZGXS010000000
VERSION     DAZGXS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..79)
                     /locus_tag="ACHKG5_004665"
     CDS             complement(<1..79)
                     /locus_tag="ACHKG5_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0870609.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHKG5_004666"
     CDS             complement(91..489)
                     /locus_tag="ACHKG5_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0870610.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHKG5_004667"
     CDS             complement(531..1556)
                     /locus_tag="ACHKG5_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0870611.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHKG5_004668"
     CDS             complement(1612..1944)
                     /locus_tag="ACHKG5_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0870612.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1954..3273)
                     /locus_tag="ACHKG5_004669"
     CDS             complement(1954..3273)
                     /locus_tag="ACHKG5_004669"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0870613.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3254..>4134)
                     /locus_tag="ACHKG5_004670"
     CDS             complement(3254..>4134)
                     /locus_tag="ACHKG5_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870614.1"
                     /translation="DGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESE
                     LDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQT
                     AQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRK
                     FVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLK
                     EVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFES
                     GLRQSTEEEKSDSRAA"
BASE COUNT          814 a         1292 c         1125 g          903 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt tacctgcagc cagcggtgcc ggtgccccct gcatcagacg
     2041 atccagcgca gtatcactgc gcgcctgtgc actctgtggt gctgcggcca gaatgcggcg
     2101 ggccgtttcc acggtcatac cgggggtttc tgccagcacg cgggcctgtt cttcgcgtcc
     2161 gtgagcctcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgcagc
     2221 ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg ttttcgccct ccgtcgctgg
     2281 caccacgcca gtaacgtcag cctgcgaagc agtggctgaa acagttgttg attgagtctc
     2341 tttggtcatt cgccctcctg agagacggga tttacgtgca tccagtgcat cacgcataac
     2401 ggtgatcgca tcggtgctgt tgacaagttc atcagccagt ccggcatcaa tggcctcctg
     2461 accgctgtac actgcagcct cggtatccag cacagcctgc acggacaggc cggtatatgc
     2521 cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc cgggactgca gtgtttcccg
     2581 gacatcaccc ggtagatggc tgtaggggtt gccatccacc ttatggctgc cgctgtaaat
     2641 cagcgtgatt tccacgccct gtttctccag ggcagcaccg taattactgt gagccatcat
     2701 gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga cgccgggagg cggcgctggc
     2761 aagcagctga cctgcactgc agttcatgtc gttggccagc gcccataccg gttttatgtc
     2821 tcgcacacgg gcgatgatgt cagcacagtc aaatgctccc gccaccatcc cgcccggtgt
     2881 gtccatatcg agcagaatgc cgtccaccat cggatcgctg gcagcctgtt gcagacgggc
     2941 gataatgccg ttgtaaccgg tcattcccga atacggctgc agcgcccgcg tccggctgac
     3001 cagcgtaccg gacaccggca gcacggcgat gccgttcatg acctgataac tccgggcctg
     3061 tcgtggtccg tcatcatcac cggataacgc cagcgccgcg ggtgcctctc cggcagtcag
     3121 gctgtcgccg gatactgcat ccgtcaggcg gctgatccca agctggcctg caagcgcaca
     3181 aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag ccatgctggc
     3241 aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt gattgtcgca
     3301 gcccggattc aaatgccgca gccgcccagg cgggcggttt aagaccagct gcgcggcgct
     3361 ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg cgtttcgcgc
     3421 actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc tgaacttctt
     3481 tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc caggcactgc
     3541 gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg cgaacgatgg
     3601 cctcttccag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg aattttcgcc
     3661 gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag tagctcatct
     3721 gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg atataccgca
     3781 gcagtgactg ctcaaacacg gagtagccgt tatccgtgtc ctgagccgtc tgcaggttca
     3841 gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc ggtgctgcgg
     3901 cgtaatacgc ggcaatttca ccaatccagc cggtcagcct ttcccgctgc tcctgactgt
     3961 tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactctca atggtggcgg
     4021 catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg tcgagcatct
     4081 tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg tcct
//
LOCUS       DAZGXS010000234         4052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGXS010000234 DAZGXS010000000
VERSION     DAZGXS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..237)
                     /locus_tag="ACHKG5_004671"
     CDS             complement(<1..237)
                     /locus_tag="ACHKG5_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0870615.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYF"
     gene            481..804
                     /locus_tag="ACHKG5_004672"
     CDS             481..804
                     /locus_tag="ACHKG5_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0870616.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1101..1370)
                     /locus_tag="ACHKG5_004673"
     CDS             complement(1101..1370)
                     /locus_tag="ACHKG5_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP0870617.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1382..2341)
                     /locus_tag="ACHKG5_004674"
     CDS             complement(1382..2341)
                     /locus_tag="ACHKG5_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP0870618.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(2434..2510)
                     /locus_tag="ACHKG5_004675"
     tRNA            complement(2434..2510)
                     /locus_tag="ACHKG5_004675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2474..2476),aa:Arg,seq:tct)
     gene            complement(2591..2666)
                     /locus_tag="ACHKG5_004676"
     tRNA            complement(2591..2666)
                     /locus_tag="ACHKG5_004676"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2631..2633),aa:Ile,seq:cat)
     gene            complement(2980..3468)
                     /locus_tag="ACHKG5_004677"
     CDS             complement(2980..3468)
                     /locus_tag="ACHKG5_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0870619.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(3459..>4052)
                     /locus_tag="ACHKG5_004678"
     CDS             complement(3459..>4052)
                     /locus_tag="ACHKG5_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP0870620.1"
                     /translation="NLMKKLETIGFDTKKDLLISVGDLVDRGTENVECLELITFPWFR
                     AVRGNHEQMMIDGLSERGNVNHWLLNGGGWFFNLDYDKEILAKALAHKADELPLIIEL
                     VSKGKKYVICHADYPCDKYEFGKPVDHQQVIWNRERISNSQDGIVKEIKGADTFIFGH
                     TPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT         1129 a          998 c          835 g         1090 t
ORIGIN      
        1 aaagtaatac tccggggctc tggtggcact gacagccacc gccttaccct cattaccggt
       61 caccgccact ggtgtggtga catcaccttc cgccgcaatg gcctgcatca gggtataagg
      121 cgtgatggcc accggactgc caaatggctg ccagccctcc ttcagttttt gtgtcagtcg
      181 ttcagcaagg tctgacggcg atgccgccct gaccacatcg tagtgtttaa atgtcataac
      241 ctctcctgcc gccagcaaag taattatccg gaacacacaa tcatgtaaca cataaaaaac
      301 acgctataaa aaaaccatgt ctcttatata acaaaaaaac gccgacaatt taaaatgccc
      361 tcttatttta aacaaagaat aaacgacacc agaaaaacaa ctcagcctga cagattgctt
      421 tcaaaacatc acctataatt tatggcatca tttaaaacct taagaaaata aggaaagcaa
      481 atgaactgga tagtgattga cacggtaatc cagccagcat gtggcatatc attttcagcc
      541 atatggtgta acataaaatt aatattatgg tatcagtcag ataccttcct ccctccgggc
      601 tgtattttta caccaacccg gacaggcgta atactcaata ataaagagct ccccgtcacc
      661 atttacaacg tggccccatt taataaaaac gtctggaatt taattaaaaa cagccaggag
      721 tgccctgcaa acacaggaaa tatcacagat aagtgtttta atcatcgctg cattctggaa
      781 atatgcccat acgggcgaaa ataacagaat cattcagata aaaagcccct ccggagaggg
      841 gctgacactg cgtatctgta tcatcatgta catggtgccg ggtgcctccc ggtgagttca
      901 gtccggtgtc cctgaacccg cgtatctcga tccaggttgt cgtcagagat gacaccttat
      961 acaccagtcg cccctccgca cagggggatt caccatgcga atttttttaa caaatgctca
     1021 gtctgacagg caactgtcaa ctgactgaat tgtgacacag attacacttg ttacccacat
     1081 accacgaatc aggttatgcc tcagtcatta ttaaactgca cttcagcaaa tccggagcct
     1141 gattcacagg tactggattt gattgtgaca gtcattcctg tcaactgagc actttgcagt
     1201 aacggttgca gattccagcg actggtccag tactcttttc cggccacttt tactgtgaat
     1261 gtatcattct cattatactt ggaaaactca attttacctt tagcgcaatc cgccgccatt
     1321 gcattaacag aaactaatgc aaataaaacc gccataaaca tcttcttcat acttaactcc
     1381 tttatttacc cgttgtatat aaaaactgtg actttctgtt cagaaacgct gcagctgtat
     1441 tactttccca taatgtattg tttattttta taacgggcct gtcgccagtt atctgacatt
     1501 ctggttgact atcttcattc acggcgcgaa cagaacgcgc cccctgatga tgacaattca
     1561 gtataacggc cacagtgccc agtatcgccg atatattatt aaaggatatt ctccccactc
     1621 tgacaccatc ctctccccga tactccggaa gcacattgct gattcgcccc cagttcagag
     1681 tgaggtccac gtctcccggc gtcatcgtat acacaggagc agtttcagac agtgcctgac
     1741 gaaattctct ctgtatctgc ctgaagcgta aggcttctgc tgtgacagtg acaaaacgca
     1801 gaactgctct ggatgcatct ctggtcattg tattaccact gaactccatt aacgccagat
     1861 atgatgaaac cagtgagtga cgactgattt gcattccgga acgttccagc gctgcgacac
     1921 gttgcagagt ggtataactg ctgtccgttg tcatggaaac cgttgtcaca ccgggcactg
     1981 atatatgtgt aaaatctgaa aaacggtaga aagtatttgt tgccgtatta acgaacccgg
     2041 ccacatataa attattttgc tcaataatca gacgaagatg gtcaaaacgc gcctgataga
     2101 catcaagccc tcgtatatcc acagcaaaat aactgcccgg tggggtgtgg ttaataacag
     2161 acaccgatgt ggtcccctga gatatatgtt caagaggggt cgatatctct gtccgtatac
     2221 tatttaacga agatacataa ctttgttgag tcgaaaagtc tatcgtaaat tcccgggaat
     2281 aggataccga agaaaagccc agtaacaggc acagtaccca tttaaataat atacacttca
     2341 tatacaaggt gttccttttg gctgaagtaa tcagcaccag acccggcgca gatataaaaa
     2401 aggcccgcaa aagcgagcca gggaaaataa gtgtggcgcg ttgtactgga ttcgaaccag
     2461 tgaccgattg cttagaaggc aattgctctg tccggctgag ctaacaacgc agaataccga
     2521 taatggaccg ccaccgggga ctccgaatct cgcacagggt gacgttcttt cctgatgagc
     2581 tagtggcggt tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
     2641 cgctctcacc actgagctaa agggccgagc gtaggataat aacgttacga aatcaatgtt
     2701 gcaagagttc acaaattcac tgattcaaaa ttattcttgt aaaaaatctt ttaacgtcag
     2761 gcacaaagag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaatccagc
     2821 atcaaatgaa ggttaattta tggacgaaaa acagttacag gctctaatga cctttatacg
     2881 attgacccaa ataaaaaaag ccaccgttgc aacttaagag tcactgacgg cagcttatgc
     2941 gaatagtgtt gctcatttgc tcaacgatgt caacacgttc tatgctacat gcttaatttt
     3001 ctctacacgt ttccggtttt taaacgcact atccagaatc gggtaaatca taaacaacga
     3061 ggcattgagg atttcgtcaa cttcccgtcg gcaggttgct agcgatggtt tttgaatgcg
     3121 cccgccgccc cggcataaca tcttgcgagg tcttgcgacg ctatgatagt aagatgcaat
     3181 ggcgtgcttg gaagagccgt gggcgtagta gctgaggagg atgccaaagg ctttcttgtc
     3241 aatgtacatg acggaatcga cgacctgaga aatcaacatt ccatcatcat cattacacat
     3301 tggccttgtc ataactcttc ccggctccac gctctccatg aatttagcta ttacgctgct
     3361 catgcgcttt tccagacgac ctgaataaac ccatgcgccc cacagttcaa gccagccatt
     3421 cagccactca tgctgttctt tggtgaggtt tagttctctt atgcccacgc gccttctccc
     3481 tgtacctgaa tcaatgtgag gtttccgcag aacactgcgc cagtatcgat atacatctgg
     3541 ttggcaaatt tgagtggttt cactgctggc gtatgaccaa agatgaacgt gtccgcgcct
     3601 ttgatttctt tcacgatccc gtcttgtgag ttgctgattc gttcgcggtt ccagattacc
     3661 tgctgatgat caactggctt tccaaactcg tatttatcac aaggataatc ggcgtggcag
     3721 atgacatatt tttttccttt actcaccagt tcgatgatta acggaagttc atctgcttta
     3781 tgggcaagag ctttagccag aatttctttg tcgtaatcga gattaaagaa ccagccaccg
     3841 ccattaagca gccagtgatt gacgtttcca cgctctgata agccatcaat catcatttgc
     3901 tcatggtttc cacgtacagc tctgaaccag gggaatgtga ttaattccag gcattctacg
     3961 ttctctgtac cgcgatcaac caaatcgccc accgagataa gcaggtcttt tttggtgtcg
     4021 aatcctatcg tctccagttt tttcatcagg tt
//
LOCUS       DAZGXS010000235         4040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGXS010000235 DAZGXS010000000
VERSION     DAZGXS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..336
                     /gene="cesT"
                     /locus_tag="ACHKG5_004679"
     CDS             <1..336
                     /gene="cesT"
                     /locus_tag="ACHKG5_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP0870621.1"
                     /translation="DEYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAG
                     AQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSS
                     DNKHYYAGR"
     gene            396..3200
                     /gene="eae"
                     /locus_tag="ACHKG5_004680"
     CDS             396..3200
                     /gene="eae"
                     /locus_tag="ACHKG5_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFP0870622.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3464..>4040)
                     /gene="escD"
                     /locus_tag="ACHKG5_004681"
     CDS             complement(3464..>4040)
                     /gene="escD"
                     /locus_tag="ACHKG5_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP0870623.1"
                     /translation="AINDVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLL
                     LNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKI
                     LAIINAMNKNSNAKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSI
                     LKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1190 a          780 c          882 g         1188 t
ORIGIN      
        1 gacgaatata tgatgattta tggtgtctgt gggaaattcc cgacagataa ctctaacttc
       61 gctcttgaga ttttgaatgc aaacttatgg tttgcagaga atggtgggcc atatctgtgc
      121 tatgaggctg gagcacaatc gctgttgtta gcgttacgtt tccctctcga tgatgctacc
      181 cctgaaaaac tcgagaatga aatagaagtc gttgttaagt caatggaaaa cctgtatttg
      241 gtattacata atcagggaat aacattagaa aacgaacata tgaaaataga ggaaatcagt
      301 tcaagcgaca ataaacatta ttacgccgga agataaaatc cgatctatta atataattta
      361 tttctcattc taactcattg tggtggagcc ataacatgat tactcatggt tgttataccc
      421 ggacccggca caagcataag ctaaaaaaaa cattgattat gcttagtgct ggtttaggat
      481 tgttttttta tgttaatcag aattcatttg caaatggtga aaattatttt aaattgggtt
      541 cggattcaaa actgttaact catgatagct atcagaatcg ccttttttat acgttgaaaa
      601 ctggtgaaac tgttgccgat ctttctaaat cgcaagatat taatttatcg acgatttggt
      661 cgttgaataa gcatttatac agttctgaaa gcgaaatgat gaaggccgcg cctggtcagc
      721 agatcatttt gccactcaaa aaacttccct ttgaatacag tgcactacca cttttaggtt
      781 cggcacctct tgttgctgca ggtggtgttg ctggtcacac gaataaactg actaaaatgt
      841 ccccggacgt gaccaaaagc aacatgaccg atgacaaggc attaaattat gcggcacaac
      901 aggcggcgag tctcggtagc cagcttcagt cgcgatctct gaacggcgat tacgcgaaag
      961 ataccgctct tggtatcgct ggtaaccagg cttcgtcaca gttgcaggcc tggttacaac
     1021 attatggaac ggcagaggtt aatctgcaga gtggtaataa ctttgacggt agttcactgg
     1081 acttcttatt accgttctat gattccgaaa aaatgctggc atttggtcag gtcggagcgc
     1141 gttacattga ctcccgcttt acggcaaatt taggtgcggg tcagcgtttt ttccttcctg
     1201 caaacatgtt gggctataac gtcttcattg atcaggattt ttctggtgat aatacccgtt
     1261 taggtattgg tggcgaatac tggcgagact atttcaaaag tagcgttaac ggctatttcc
     1321 gcatgagcgg ctggcatgag tcatacaata agaaagacta tgatgagcgc ccagcaaatg
     1381 gcttcgatat ccgttttaat ggctatctac cgtcatatcc ggcattaggc gccaagctga
     1441 tatatgagca gtattatggt gataatgttg ctttgtttaa ttctgataag ctgcagtcga
     1501 atcctggtgc ggcgaccgtt ggtgtaaact atactccgat tcctctggtg acgatgggga
     1561 tcgattaccg tcatggtacg ggtaatgaaa atgatctcct ttactcaatg cagttccgtt
     1621 atcagtttga taaatcgtgg tctcagcaaa ttgaaccaca gtatgttaac gagttaagaa
     1681 cattatcagg cagccgttac gatctggttc agcgtaataa caatattatt ctggagtaca
     1741 agaagcagga tattctttct ctgaatattc cgcatgatat taatggtact gaacacagta
     1801 cgcagaagat tcagttgatc gttaagagca aatacggtct ggatcgtatc gtctgggatg
     1861 atagtgcatt acgcagtcag ggcggtcaga ttcagcatag cggaagccaa agcgcacaag
     1921 actaccaggc tattttgcct gcttatgtgc aaggtggcag caatatttat aaagtgacgg
     1981 ctcgcgccta tgaccgtaat ggcaatagct ctaacaatgt acagcttact attaccgttc
     2041 tgtcgaatgg tcaagttgtc gaccaggttg gggtaacgga ctttacggcg gataagactt
     2101 cggctaaagc ggataacgcc gataccatta cttataccgc gacggtgaaa aagaatgggg
     2161 tagctcaggc taatgtccct gtttcattta atattgtttc aggaactgca actcttgggg
     2221 caaatagtgc caaaacggat gctaacggta aggcaaccgt aacgttgaag tcgagtacgc
     2281 caggacaggt cgtcgtgtct gctaaaaccg cggagatgac ttcagcactt aatgccagtg
     2341 cggttatatt ttttgatcaa accaaggcca gcattactga gattaaggct gataagacaa
     2401 ctgcagtagc aaatggtaag gatgctatta aatatactgt aaaagttatg aaaaacggtc
     2461 agccagttaa taatcaatcc gttacattct caacaaactt tgggatgttc aacggtaagt
     2521 ctcaaacgca agcaaccacg ggaaatgatg gtcgtgcgac gataacacta acttccagtt
     2581 ccgccggtaa agcgactgtt agtgcgacag tcagtgatgg ggctgaggtt aaagcgactg
     2641 aggtcacttt ttttgatgaa ctgaaaattg acaacaaggt tgatattatt ggtaacaatg
     2701 tcagaggcga gttgcctaat atttggctgc aatatggtca gtttaaactg aaagcaagcg
     2761 gtggtgatgg tacatattca tggtattcag aaaataccag tatcgcgact gtcgatgcat
     2821 cagggaaagt cactttgaat ggtaaaggca gtgtcgtaat taaagccaca tctggtgata
     2881 agcaaacagt aagttacact ataaaagcac cgtcgtatat gataaaagtg gataagcaag
     2941 cctattatgc tgatgctatg tccatttgca aaaatttatt accatccaca cagacggtat
     3001 tgtcagatat ttatgactca tggggggctg caaataaata tagccattat agttctatga
     3061 actcaataac tgcttggatt aaacagacat ctagtgagca gcgttctgga gtatcaagca
     3121 cttataacct aataacacaa aaccctcttc ctggggttaa tgttaatact ccaaatgtct
     3181 atgcggtttg tgtagaataa ttccataacc accccggcta aaatatgtat tgttttagtc
     3241 ggggcataat tatttcttct taagaaataa cctcttataa tcaaatctac tactggtctt
     3301 tttatctgct taataggtct ctttcaaaga gacacattca cgttttctca tgtaggttga
     3361 tccaaccacg ctgataccaa agctgaatca catcaagcaa caactatgct cacaacatcc
     3421 acacaataaa aaaccctccg aagaggggga agaggataag aaattaatac gacagtggaa
     3481 tatgtatata ttcgtctggg cgggagatgt tgataccatc tttaggatca atcgcgtcta
     3541 tgacatagcc tcctttcaga atgctaccaa tccccaggct ggtgccatta tcaagagcaa
     3601 ttgttggatt cttcatcgta ccgctgattc tgagtatttt tccggggaaa atatctgctg
     3661 aaatgtacgg ctgaatgttc tgaaagagta tcttggcgtt actatttttg ttcattgcat
     3721 ttataatggc aaggatctta ctctcattct gttgagaaac ttcccccgca acaatgatga
     3781 catcattctt tttgaaaata ttgacatagt ttattagttt attcttttta aactctgatg
     3841 ccagttcatt gattttattg ttactgttgt tgtgtatttc ccatcctgca actcctggcg
     3901 tattcagtaa cagattttca acctcgctcc atttgggtga agggggaatg tatcccgata
     3961 acagaaagaa tccaggctta ttaccttttg taataatgat atctttgtaa ccgtattccg
     4021 ttaagacatc attaattgcg
//
LOCUS       DAZGXS010000236         3987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGXS010000236 DAZGXS010000000
VERSION     DAZGXS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            232..2148
                     /gene="dnaK"
                     /locus_tag="ACHKG5_004682"
     CDS             232..2148
                     /gene="dnaK"
                     /locus_tag="ACHKG5_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0870624.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2237..3367
                     /gene="dnaJ"
                     /locus_tag="ACHKG5_004683"
     CDS             2237..3367
                     /gene="dnaJ"
                     /locus_tag="ACHKG5_004683"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0870625.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3471..3623)
                     /gene="mokC"
                     /locus_tag="ACHKG5_004684"
     CDS             complement(3471..3623)
                     /gene="mokC"
                     /locus_tag="ACHKG5_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0870626.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1058 a          986 c         1050 g          893 t
ORIGIN      
        1 aaattgctgt caattttacg tcttgtcctg ccatatcgcg aaatttctgc gcaaaagcac
       61 aaaaaatttt tgcatctccc ccttgatgac gtgggttacg accccattta gtagtcaacc
      121 gcagtgagtg agtctgcaaa aaaatgaaat tgggcagttg aaaccagacg tttcgcccct
      181 attacagact cacaaccaca tgatgaccga atatatagtg gagacgttta gatgggtaaa
      241 ataattggta tcgacctggg tactaccaac tcttgtgtag cgattatgga tggcaccact
      301 cctcgtgtgc tggagaacgc cgaaggcgat cgcaccacgc cttctatcat tgcctatacc
      361 caggatggtg aaactctggt tggtcagccg gctaaacgtc aggcagtgac gaacccgcaa
      421 aacaccctgt ttgcgattaa acgcctgatt ggccgccgct tccaggacga agaagtacag
      481 cgtgatgttt ccatcatgcc gttcaaaatt attgctgctg ataacggcga cgcatgggtc
      541 gaagttaaag gccagaaaat ggcaccgccg cagatttctg ctgaagtgct gaaaaaaatg
      601 aagaaaaccg ctgaagatta cctgggtgaa ccggtaactg aagctgttat caccgtaccg
      661 gcatacttta acgatgctca gcgtcaggca accaaagacg caggccgtat cgctggtctg
      721 gaagtaaaac gtatcatcaa cgaaccgacc gcagctgcgc tggcttacgg tctggacaaa
      781 ggtactggca accgtactat cgcggtttat gacttgggtg gtggtacttt cgatatttct
      841 attatcgaaa tcgacgaagt tgacggcgaa aaaaccttcg aagttctggc aaccaacggt
      901 gatacccacc tgggtggtga agacttcgac agccgtctga tcaactatct ggttgaagaa
      961 ttcaagaaag atcagggcat tgacctgcgc aacgatccgc tggcaatgca gcgcctgaaa
     1021 gaagcggcag aaaaagcgaa aatcgaactg tcttccgctc agcagaccga cgttaacctg
     1081 ccgtacatca ctgcagacgc gaccggtccg aaacacatga acatcaaagt gactcgtgcg
     1141 aaactggaaa gcctggttga agatctggtt aaccgttcca tcgagccgct gaaagttgcg
     1201 ctgcaggacg ctggcctgtc cgtatctgat atcgacgacg ttatcctcgt tggtggtcag
     1261 actcgtatgc caatggttca gaagaaagtt gctgagttct ttggtaaaga gccgcgtaaa
     1321 gacgttaacc cggacgaagc tgtagcaatc ggtgctgctg ttcagggtgg tgttctgact
     1381 ggtgacgtaa aagacgtact gctgctggat gttaccccgc tgtctctggg tatcgaaacc
     1441 atgggcggtg tgatgacgac gctgatcgcg aaaaacacca ctatcccgac caagcacagc
     1501 caggtgttct ctaccgctga agacaaccag tctgcggtaa ccatccatgt gctgcagggt
     1561 gaacgtaaac gtgcggctga caacaaatct ctgggtcagt tcaacctgga tggtatcaac
     1621 ccggcaccgc gcggcatgcc gcagatcgaa gttaccttcg atatcgatgc tgacggtatc
     1681 ctgcacgttt ccgcgaaaga taaaaacagc ggtaaagagc agaagatcac catcaaggcg
     1741 tcttctggtc tgaacgaaga tgaaatccag aaaatggtac gcgacgccga agctaacgcc
     1801 gaagctgacc gtaagtttga agagctggta cagactcgca accagggcga ccatctgctg
     1861 cacagcaccc gtaagcaggt tgaagaagca ggcgacaaac tgccggctga cgacaaaact
     1921 gctatcgagt ctgcgctgac tgcactggaa actgctctga aaggtgaaga caaagccgct
     1981 atcgaagcga aaatgcagga actggcacag gtttcccaga aactgatgga aatcgcccag
     2041 cagcaacatg cccagcagca gactgccggt gctgatgctt ctgcaaacaa cgcgaaagat
     2101 gacgatgttg tcgacgctga atttgaagaa gtcaaagaca aaaaataatc gccctataaa
     2161 cgggtaatta tactgacacg ggcgaagggg aatttcctct ccgcccgtgc attcatctag
     2221 gggcaattta aaaaagatgg ctaagcaaga ttattacgag attttaggcg tttccaaaac
     2281 agcggaagag cgtgaaatca aaaaggccta caaacgcctg gccatgaaat accacccgga
     2341 ccgtaaccag ggtgacaaag aggccgaggc gaaatttaaa gagatcaagg aagcttatga
     2401 agttctgacc gactcgcaaa aacgtgcggc atacgatcag tatggtcatg ctgcgtttga
     2461 gcaaggtggc atgggcggcg gcggttttgg cggcggcgca gacttcagcg atatttttgg
     2521 tgacgttttc ggcgatattt ttggcggcgg acgtggtcgt caacgtgcgg cgcgcggtgc
     2581 tgatttacgc tataacatgg agctcaccct cgaagaagct gtacgtggcg tgaccaaaga
     2641 gatccgcatt ccgactctgg aagagtgtga cgtttgccac ggtagcggtg caaaaccagg
     2701 tacacagccg cagacctgtc cgacctgtca tggttctggt caggtgcaga tgcgccaggg
     2761 tttctttgcc gtgcagcaga cctgtccaca ctgtcagggc cgcggtacgc tgatcaaaga
     2821 tccgtgcaac aaatgtcatg gtcatggtcg tgttgagcgc agcaaaacgc tgtccgttaa
     2881 aatcccggca ggggtggaca ctggagaccg catccgtctt gcgggcgaag gtgaagcggg
     2941 tgaacacggc gcaccggcag gcgatctgta cgttcaggtt caggttaaac agcacccgat
     3001 tttcgagcgt gaaggcaaca acctgtattg cgaagtcccg atcaacttcg ctatggcggc
     3061 gctgggtggt gaaatcgaag taccgaccct tgatggtcgc gtcaaactga aagtgcctgg
     3121 cgaaacccag accggtaagc tattccgtat gcgcggtaaa ggcgtcaagt ctgtccgcgg
     3181 tggcgcacag ggtgatttgc tgtgccgcgt tgtcgtcgaa acaccggtag gcctgaacga
     3241 gaagcagaaa cagctgctgc aagagctgca agaaagcttc ggtggcccaa ccggcgagca
     3301 caacagcccg cgctcaaaga gcttctttga tggtgtgaag aagttttttg acgacctgac
     3361 ccgctaacct ccccaaaagc ctgcccgtgg gcaggcctgg gtaaaaatag ggtgcgttga
     3421 agatatgcga gcacctgtaa agtggcgggg atcactcccc gccgttgctc ttactcggat
     3481 tcgtaagccg tgaaaacagc aacctccgtc tggccagttc ggatgtgaac ctcacagagg
     3541 tcttttctcg ttaccagcgc cgccactacg gcggtgatac agatgacgat cagggcgaca
     3601 atcatcacct tatgctgctt cattgctctc ttctccttga ccttacggtc agtaagaggc
     3661 actctacatg tgttcagcat atagggggcc tcgggttgat ggtaaaatat cactcggggc
     3721 ttttctctat ctgccgttca gctaatgcct gagacagaca gcctcaagca cccgccgcta
     3781 ttatatcgct ctctttaacc cattctgttt tatcgattct aatccggaag acgcctcgca
     3841 tttttatggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tcttcatcgc
     3901 aattattgac gacaagctgg attatttttg aaatattggc ctaacaaaca tcgccgactg
     3961 acaacaaatt aattattact tttccta
//
LOCUS       DAZGXS010000237         3839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGXS010000237 DAZGXS010000000
VERSION     DAZGXS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..168
                     /locus_tag="ACHKG5_004685"
     CDS             <1..168
                     /locus_tag="ACHKG5_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870627.1"
                     /translation="ASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLHQN
                     NTHSTTLTTTV"
     gene            218..2338
                     /gene="ehxB"
                     /locus_tag="ACHKG5_004686"
                     /gene_synonym="hlyB"
     CDS             218..2338
                     /gene="ehxB"
                     /locus_tag="ACHKG5_004686"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP0870628.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            2342..3781
                     /gene="ehxD"
                     /locus_tag="ACHKG5_004687"
     CDS             2342..3781
                     /gene="ehxD"
                     /locus_tag="ACHKG5_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP0870629.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         1233 a          602 c          808 g         1196 t
ORIGIN      
        1 gcatctgaaa aaaacatggt taatatcacc agtgttgcca atgatattaa taagattatt
       61 tcttcagttt cagggttcga ttcaggtgat gaacgattag catctttata taatttatcc
      121 ttacatcaaa acaacacaca ctcaacaact ttaacgacaa ctgtctgaga ctaaaagagg
      181 aggtgatggc cattccagga ggaatggtca tcgttatatg atgagtaaat gtagttctca
      241 taatagtctg tatgcactga tattgcttgc acaatatcat aatataactg tcaatgctga
      301 aactataagg catcagtata atacccacac acaagatttt ggggtgactg aatggttact
      361 ggcagcgaaa tctattggct taaaagcaaa atatgtagaa aaacattttt ccagattgtc
      421 aataatttct ttacctgcgt tgatatggcg ggatgacggt aagcattata tattgtctcg
      481 tattactaaa gattcatcac gctatcttgt ttatgatcca gaacaacatc agtcactaac
      541 ttttagtcgg gatgagtttg aaaaactgta tcagggaaaa gtcattctgg ttacgtcaag
      601 agcaacagta gtcggagagt tagctaaatt tgatttttct tggtttatcc cctctgttgt
      661 gaaatacagg aggattttac ttgaggtgtt aactgtttct gcttttattc agtttcttgc
      721 gttaataaca cctctttttt ttcaggttgt aatggataag gttttagttc accgggggtt
      781 ttcaacgtta aatattatca caatagcatt tattatagtg atactttttg aagtgatatt
      841 aaccggagcc agaacttata ttttctctca tactacaagt cgtattgacg tcgaactggg
      901 tgctaagtta ttcagacatt tgcttgcatt gcctgtttca tattttgaaa atcgcagggt
      961 cggagagacc gttgccagag taagggaact ggagcaaatt cgtaattttt taaccggaca
     1021 agcgttgaca tcagttcttg atctattttt ttctgtaata tttttttgtg tcatgtggta
     1081 ttacagccct caattaacac tggttatatt attgtcacta ccttgttatg ttatatggtc
     1141 attgtttata tcacccttat tacgtcgacg tcttgatgat aagtttctca ggaatgcaga
     1201 aaatcaagct tttcttgtcg aaacggtaac agcaataaat acaatcaaat ccatggcagt
     1261 atcaccacag atgattgcta catgggataa acaactggcc gggtatgttg cttccagttt
     1321 cagggtaaat ctggttgcaa tgacagggca gcaggggata cagctgatac agaaaagtgt
     1381 aatggtaatt agtctatgga tgggagcaca tcttgtcata tcgggagaga taagcatcgg
     1441 gcagttaatc gcgtttaata tgcttgcagg tcaggtcatt gctcctgtta tcagactggc
     1501 tcatctttgg caggattttc agcaggttgg tatttctgtt gagcgccttg gtgatgtatt
     1561 aaatacaccg gtagagaaaa agtcaggcag aaatatactg ccggaaattc agggggatat
     1621 cgaatttaaa aatgtcagat ttcggtattc ttctgacggt aatgttattt tgaataatat
     1681 taatttatac atatcaaagg gggatgttat cggtatagtt ggccgttctg gttcaggaaa
     1741 aagtacatta acaaaactgc ttcagcgctt ttatatacca gagaccggac agattttaat
     1801 tgatgggcat gatttatcac ttgcagatcc agaatggtta cgacgccaga ttggtgttgt
     1861 attgcaggaa aatatactat taaatcgtag tattatcgat aatattacat tagcttctcc
     1921 ggctgtatct atggaacagg ctattgaggc agccagactt gcaggtgccc atgattttat
     1981 tagagaacta aaagaagggt acaatactat tgttggagaa cagggcgttg gtctttcagg
     2041 agggcagcgc caacggatcg caatagcccg ggctcttgtt acaaatcctc gaattcttat
     2101 ttttgatgaa gcaaccagtg ctcttgatta tgagtcggaa aatataataa tgaaaaatat
     2161 gtcaagaata tgtaagaaca gaaccgtaat tattattgca cacaggttgt caactgtaaa
     2221 aaatgcaaac agaataattg ttatggataa tggctttatt tctgaagatg gcacacataa
     2281 agagcttatc tccaaaaaag acagtttata tgcatattta tatcagttgc aggcataacg
     2341 tatgcgcttt tacatgaaag gtttatggga tttggtttgc cgttataaga cggttttttc
     2401 tgatgtctgg aaaattcgtc acacacttga tgctcctgtg agagaaaaag atgagtatgc
     2461 atttcttcct gctcaccttg aactcattga aacaccagta tccagacgtt ctcattttgt
     2521 tgtatggagt attttattat ttgtaattat atctcttctc ttatctgttc ttgggaaagt
     2581 tgaagtggtt tcagtagcaa atggtaagtt tactcatagt ggaagaagca aagaaataaa
     2641 accgatagag aatgcgattg tagaaaaaat aatggtaaaa gatggctctt ttgtaaaaaa
     2701 aaatgatcca ttagttgaat tgacggtgcc tggtgttgaa tctgatattt taaaatcaga
     2761 agcatctttg ttgtatgaaa aaacagaaca atatcgctac gcaattcttt ctgaatcaat
     2821 acaacggaat gagcttcctg aaataaggat aacagatttt cctggcggag aagataatgc
     2881 gggaggtgaa cattttcaga gggttagctc attaataaaa gagcaattca tgacatggca
     2941 gaacagaaaa aatcaaaagc agctcacatt aaataaaaaa atagttgaac gggatgcagc
     3001 gcttgctcgt gttagcctgt atgagcatca ggtatcacag gaaggaagaa aactcaatga
     3061 ttttaagtat ttgttgaata aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa
     3121 tagctatatt caggcaaaaa atgaacatgc agtctggctt gcacaggttt ctcaacttga
     3181 aaaagaaata gaacttgtgc gggaagaact ggcactggag acgaatatct tcagaagtga
     3241 aattatcgag aagcacagaa aatcaacaga taacattgtg ttgctggagc atgagcttga
     3301 aaaaaacagg cagagaaaag catcatcttt tattaaagct cctgtgagtg gtactgttca
     3361 ggagttaaat atacatacag aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat
     3421 tgtccctgat aatgatattc tcgaagtaac agcatctgta cttaacaagg atatcggttt
     3481 tatacaacct ggacaggagg tcgttattaa agtagatgca tatccatata cacgtcatgg
     3541 ttaccttaca gggaaagtaa aaaatataac tgcagattct gtttctgttc cggatacagg
     3601 gcttgtattt aacgtgatta tatctgttga tcggaatgat atacagggag aaagaaaaaa
     3661 aatccctgtt acagctggaa tgactgttat ggcagaaata aaaaccgggg tgcgtagtgt
     3721 tatcagctat cttcttagtc cattaaagga aactattaat gaaagtttac gtgaacgtta
     3781 gtatataaat aacatattgg ctttttatat gatatagatt tctgaaataa tcttgtaaa
//
LOCUS       DAZGXS010000238         3826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGXS010000238 DAZGXS010000000
VERSION     DAZGXS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            76..303
                     /locus_tag="ACHKG5_004688"
     CDS             76..303
                     /locus_tag="ACHKG5_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870630.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(380..622)
                     /gene="yfdY"
                     /locus_tag="ACHKG5_004689"
     CDS             complement(380..622)
                     /gene="yfdY"
                     /locus_tag="ACHKG5_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0870631.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            975..1895
                     /gene="lpxP"
                     /locus_tag="ACHKG5_004690"
     CDS             975..1895
                     /gene="lpxP"
                     /locus_tag="ACHKG5_004690"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0870632.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2251..2322
                     /gene="ypdK"
                     /locus_tag="ACHKG5_004691"
     CDS             2251..2322
                     /gene="ypdK"
                     /locus_tag="ACHKG5_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0870633.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHKG5_004692"
     CDS             complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHKG5_004692"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0870634.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          988 a          966 c          890 g          982 t
ORIGIN      
        1 agtgaaccat tttcaggata actttctatg aaagtaaact taatactttt tagcttattt
       61 ttattggtct ctattatggc atgcaatgtt tttgcatttt ctaattctgg tggtggtagt
      121 gagacgagct ataaagagcc agaaaaaacg tcagcgatga cgacaactca ctctacaaaa
      181 tttcagccat cacaggcgat tttgtttaag atgagagaag atgcaccacc attaaacctc
      241 acagaagaaa ccacgccccc ttttccgaca aaggcgaatt atcttattca tcctgtgcga
      301 tagcgtctgt cacctggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca
      361 aatgaaccat cctgacccac tatctcctgc cataccccac cataaagcga atcatcaagc
      421 cacaagccgg ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc
      481 acatggtgaa gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg
      541 ccgataccac gttcagtacc tgaccgatgt atccgcttac gcagacaata cacagggcga
      601 gaaacatcca taaataaaac atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc
      661 agtcacttta atagtaagac aatccttaac ctctggcaat ttttgctaag cgtcaatgaa
      721 aacagcatgt taaatgcaat actattgcgt gcggaaaatt ttttactttg cacgattaat
      781 aatcagtagc tgaaagcagt cagccagtga taaagtacag ataccgcatt catgctcctc
      841 gtggttatgt cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc
      901 ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc
      961 tacagaaggt aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct
     1021 actggctcac atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg
     1081 ttctctgctt tctcggcacg cgtattggcg caatggcgcg accattcctg aaacggcgtg
     1141 aatctatcgc ccgtaaaaac ctggaacttt gcttcccgca gcattctgcg gaagaacgcg
     1201 agaagatgat tgccgaaaac tttcgttcac tcggcatggc tctggtagaa accggcatgg
     1261 cgtggttctg gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ctggataacc
     1321 ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc
     1381 tggaactggg cggccgcgtg atgggacttt gtcagccaat gatggctacc tatcgtccac
     1441 ataataatca gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga
     1501 tgatcggcag aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat
     1561 ggtttgctcc cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg
     1621 tggaaaatgt cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca
     1681 tgttgaccgt aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc
     1741 cagagatgga aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta
     1801 tcgagaaaga gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa
     1861 cgcgcccggt gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc
     1921 tcaaatcggc cataataacc tcatggtttt tatggctttt ttcttaagat tttttccttc
     1981 gctgaatatc taattattac cgccagaaat taaactgtcg ccgatatgag acacctgtaa
     2041 gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag
     2101 cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacaggac
     2161 ttttaaggtc gttttgcccg gtgtcaactc actgtatcag gtgtgatgaa gtcattcagg
     2221 cgtaaccgta attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt
     2281 atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga
     2341 aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt
     2401 cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc
     2461 cacgaatcgc ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtgt
     2521 cgccgtagtc accaaagcca atccctggag agacacagac cttcgcttcg ttaaggagct
     2581 tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat
     2641 acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata
     2701 catcgcggcg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcca
     2761 gcgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga
     2821 ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg
     2881 ccatgttgta gcttttcgac agcgtaaaga actcgactgc cacatcgcgt gcacccggta
     2941 cctgcatgat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat
     3001 ggaccaccag cacatcgtag cgtttcgcca gcgccactac tttttcgaag aactccagtt
     3061 ccacgcattg cgcggttggg tttgacggga agccgaggat catcatcttc ggtttcggat
     3121 aactttcaag aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca
     3181 ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg tagcttggat
     3241 tcggcaccag caccgtgtca ccatgatcca gcgtcgccaa catcaggtgt gccagccctt
     3301 ctttcgaacc aatggtgacg atggcttctg attccggatc gatttcaacg tcgtagcgat
     3361 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac
     3421 catgcgtgtc cggacgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg
     3481 caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca
     3541 ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc
     3601 gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc
     3661 cgagcgacgc tgccacgatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
     3721 cagcgtttta gaacattttt attaatgacg ttaaatgagg aactctgctt taaaaacagg
     3781 aaatttatct gcgctaacaa gaaaattacc actcatttta ttaaca
//
LOCUS       DAZGXS010000239         3818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGXS010000239 DAZGXS010000000
VERSION     DAZGXS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            53..346
                     /gene="bor"
                     /locus_tag="ACHKG5_004693"
     CDS             53..346
                     /gene="bor"
                     /locus_tag="ACHKG5_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP0870635.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(437..619)
                     /gene="rzoD"
                     /locus_tag="ACHKG5_004694"
     CDS             complement(437..619)
                     /gene="rzoD"
                     /locus_tag="ACHKG5_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP0870636.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(836..1312)
                     /locus_tag="ACHKG5_004695"
     CDS             complement(836..1312)
                     /locus_tag="ACHKG5_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0870637.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1299..1604)
                     /locus_tag="ACHKG5_004696"
     CDS             complement(1299..1604)
                     /locus_tag="ACHKG5_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0870638.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1926..2615)
                     /locus_tag="ACHKG5_004697"
     CDS             complement(1926..2615)
                     /locus_tag="ACHKG5_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0870639.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(2612..2752)
                     /locus_tag="ACHKG5_004698"
     CDS             complement(2612..2752)
                     /locus_tag="ACHKG5_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0870640.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2749..3111)
                     /gene="rusA"
                     /locus_tag="ACHKG5_004699"
     CDS             complement(2749..3111)
                     /gene="rusA"
                     /locus_tag="ACHKG5_004699"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0870641.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3108..3398)
                     /locus_tag="ACHKG5_004700"
     CDS             complement(3108..3398)
                     /locus_tag="ACHKG5_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0870642.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3391..3561)
                     /locus_tag="ACHKG5_004701"
     CDS             complement(3391..3561)
                     /locus_tag="ACHKG5_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0870643.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3561..>3818)
                     /locus_tag="ACHKG5_004702"
     CDS             complement(3561..>3818)
                     /locus_tag="ACHKG5_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870644.1"
                     /translation="KSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTIQSLRHTSD
                     LGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
BASE COUNT          944 a          987 c          888 g          999 t
ORIGIN      
        1 attttctaca catacgattc tgcgaacttt aaaaagcatc gggaataaca ccatgaaaaa
       61 aatgctactc gctactgcgc tggccctgct tattacagga tgtgcccaac agacgtttac
      121 tgttcaaaac aaaccggcag cagtaacacc aaaggaaacc atcacccacc atttcttcgt
      181 ctctggaatt gggcagaaga aaactgtcga tgcagctaaa atttgtggcg gcgcagaaaa
      241 tgttgttaaa acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt ttattacttt
      301 aggcatttat actccgctgg aagcgcgtgt gtattgctca caataattgc atgagctgcc
      361 catcgatatg ggcagctcta tctgcactgc tcattaatat acttctgggt tccttccagt
      421 tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct
      481 gccagtcggg gggaggctgc attatccacg ccggaggcgg tggtggcttc acgcactgac
      541 tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc atgcagagca
      601 tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag cgcagcaaca
      661 tcacgctgac gcatctgcat gtcagtaatt gccacgttcg ccagcttcag ttctctggca
      721 tttttgtcgc gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt aacagcccat
      781 gacaggcaga cgatgatgca gatgagcaga gcggagataa tcgcggttat tctgctcata
      841 cctcactctc tctgaccgtt ccgccagctt ctttgaattt tgcaatcagg ctgtcagcct
      901 tatgctcgaa ctgaccataa ccagcgcccg gcagtgaagc ccagatatta ctgcaacggt
      961 cgattgcctg acggatatca ccgcgatcaa tcataggtaa agcaccacgc tctttaatct
     1021 gctgcaatgc cactgcgtcc tggcttttgg gggagaagtc tttcaaacca agctgtttac
     1081 ggtaagcatc ccaccagcgt gaaagaagct gataacgtcc ggcggctgtt gatttgagtt
     1141 tggggtgtag cgtgacaagt ttgcgagggt gatcggagta atcagtaaac agttcgccgc
     1201 caacaataac atcataaccg tggttacgtg tcggttgtcg cccgttatcc gttccttctg
     1261 accacgccaa catatcgagg aaggctttac gctgaggatt aagattttgc atttttcacc
     1321 cctgtcagtc gttcccagaa gtacgtcagt gcaaccgaac ccatcgcacc actaatcccc
     1381 gctgtcgcga gaatcatgta aatactgaat ccactttcga tgctgatcag gccaccaata
     1441 acaccggtga atcctgatac cactatttga gccagagcat ttatccaact ccacgttgct
     1501 ttactctgct tcacatctat caggtagcgg accagaccgc cccaacctgc gatgatcagc
     1561 aaaacgagcc agaacgctcc ggcaaggctc tctttttcgt gcatatgaat agccaatgtt
     1621 tcgccgccga caaaaggccg ggacgttaaa tgtcagaaat caggctcacg gggtaattta
     1681 acgacaaagc acggagttga tgctccccac aagccaggaa taaaaaagcc agcatgtagc
     1741 tggcaacaga gggctaagca atatcaactc aacagctgaa gaccccctgg ctggggtagg
     1801 ttggaaggct actcaccgtt cagaaacaga aaagcccaag gctttaaacc tcgggcttga
     1861 atttggatta ctgccagtgc gtacaacatt ggcaaaatat cagatttaca taaaatatat
     1921 gatttttaat ccagttttgc aatatcttgc tgtgaaaatg tggtcttttg ttttgaacgt
     1981 gttttcgtta aaagcaataa agcttggcta tcaagctgta gaaaaatgtg tttcattgca
     2041 acccagcgtt cagtaaatgt ctcagaccag ttttttgatg tcactcccac cagtgatgcc
     2101 agctcctggt attcataggt cttacgccct gccagctcgt tcttcacatc ctgtgccgcc
     2161 agccagatca acttcttcaa acgttccagt gtcttacctg caattttcct ggtacccaac
     2221 agagtcttaa actcgctcca tgcccactgc gttatggtga cctgatgttc ccagcgaaca
     2281 ctttcgctgt aactccacag caaccacgct ttctgatgtt cttcgagaga cagaaccgcg
     2341 cggcgccatg acgaggttga gaactcaacc tggctgacca gtgcaatgga tgaacctttt
     2401 gcgtacgact gcttaccgga aatcggcgga ttatccagcg taatcatcct gccagttacc
     2461 tcatccagaa tgcgcggctt ctttcgtttg tatgtaccag tatcaaattg tgcatgctcc
     2521 agccaggctt caagctgtcc tttcgttgct ccgctcaaat cagcggtagc cacaatgagt
     2581 tgctcgcgga catactgtaa atattgggta ttcatgcggc agctcctttc agtgttttgg
     2641 cgtaattctt cagtatccgg taatcggtca aaacagaacc aggaaaacga tataagcgca
     2701 ggcgcatcca gcggtggcga agacgttctg ccatataaga ctcaaacatc attcattccc
     2761 cagttcggtg atggtcagct ccagcttccc acctttggta acgggcatct tcacaacacg
     2821 gtaatcaacg acctgagcat catccagcca gaaacctgct ttggtgagtg cgtcaaaagc
     2881 ggctttttgc agattatcca ggtcacggcg acggcgatcc ggcatgtggc actcaatacg
     2941 gattttcact ggcatagcca ggccgatatc cagcatggag cctttgatga ttcgggcgac
     3001 gttatcacgg tatgcctgcc cttctgcgct gatgtgcgtg cgcccgcgat tatgccggta
     3061 gtagcgatta ttgctcggag gccagggtaa tgtgatgctg taggtattca cgccttaata
     3121 accccctctt tcagccagat aacctgtgtt ctcgccatac cttccagcgc gcattctttt
     3181 gcatatgcag catcgacaaa atgtgtgcgg cggtcgattt cgtcgtggca ggcagaacat
     3241 gcaatggtgg caatcaagtc tggcggtttg gtaccggtgc cgcacaatcc agtcagccgg
     3301 atatgtgcca gtacagacgt ttcagggttg ccattacata cgccagggat tcttacctgg
     3361 cattcccgac cacgcgctgc ttttctcaaa tcagccatga ttcctccttg ctgccagtcg
     3421 caaccatttt ttatcaacca ggctggcggt atatccgagc agtgttggta tttcggatgg
     3481 cttcagctca ggtttacgct tacgacgatt tggtactttg tagatgtgtc cgttcatgac
     3541 acgaataagc ggtgtagcca ttacgcctcc tgcttgtcac gcagcagctg gaactcgcag
     3601 ctctgtggaa tagtcaggtg gcagccaata ttcatcgccc aggcttcaac cttacacagg
     3661 aagacataca tctctccggt accaagatcg gaggtatggc gtaacgactg gatagtagtg
     3721 atttcgccgg ttacgacatc aaccaggtcc ttggtttcat aaccgaggta tgtgtgtttg
     3781 agagcatctt ttacccatgc tgaagtagcg aacgattt
//
LOCUS       DAZGXS010000240         3789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGXS010000240 DAZGXS010000000
VERSION     DAZGXS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..125
                     /locus_tag="ACHKG5_004703"
     CDS             <1..125
                     /locus_tag="ACHKG5_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870645.1"
                     /translation="VKGMEGAINAKTVTYDFERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(239..1381)
                     /locus_tag="ACHKG5_004704"
     CDS             complement(239..1381)
                     /locus_tag="ACHKG5_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0870646.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(1371..1607)
                     /locus_tag="ACHKG5_004705"
     CDS             complement(1371..1607)
                     /locus_tag="ACHKG5_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0870647.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            1747..2535
                     /locus_tag="ACHKG5_004706"
                     /pseudo
     CDS             1747..2535
                     /locus_tag="ACHKG5_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            2532..3233
                     /locus_tag="ACHKG5_004707"
     CDS             2532..3233
                     /locus_tag="ACHKG5_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0870648.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            3230..3532
                     /locus_tag="ACHKG5_004708"
     CDS             3230..3532
                     /locus_tag="ACHKG5_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP0870649.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            3600..>3789
                     /locus_tag="ACHKG5_004709"
     CDS             3600..>3789
                     /locus_tag="ACHKG5_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFP0870650.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIW"
BASE COUNT         1016 a          826 c          918 g         1029 t
ORIGIN      
        1 ttgttaaagg tatggaaggc gcaatcaacg ccaagaccgt aacctatgac ttcgaacgtc
       61 tgatggaagg cgctaagctg ctgaaatgtt cagaatttgg tgatgcgatc atcaagaaca
      121 tgtaatcact acatgtgtta aatattgaaa cgggcgtata acacgcccgt tgttttatga
      181 tgatttaaaa tcttccccaa aactttcccc aaaacccttc cccaaaactg gctattttct
      241 atgctgtttt gatatctacg ataatccagt ctttaccacg atcatcattg tatcggtcgg
      301 tcatttttct ggatttatga cctaacaact tttgcgtatc cagaccctgt tcccgatata
      361 accgttctga cagagatcgc tgctcatgaa atgtgggcgc agttccttgc tcccatttta
      421 tgccacattt ttccctggcc tttttaaaag ccgttgtaag agtatttgca gacacctggt
      481 ctcctctgtt tgcttgagag gtagtgtgac ggtaatggac cagatattta ctaacaacag
      541 catccctgca ctgagatata acttcacgaa gggtaatatt cagagcatcg catttcaggt
      601 taagcggaat agcaagtttt gaaccggttt tttcctgagt aatgtgcaac atgtcgtccc
      661 agatatcaga gaatttcaaa ttgcagatat cgcttaaacg ttgtccagtg acaagagcaa
      721 gtagcatccc gcattttaaa tagggctgcc gtctgcttac gctgtcaaat attgcctgcc
      781 attcgggcag tgataatctt tggcggttta ctcgatttcg cggttgtttt gttgcctgcg
      841 ctgggttaaa tcctggcgga acatgtcctg cgtgttgtgc ttctttgaag acgtcgatca
      901 acaccattct cacgacttgc gccatcctgt tatgaccttc agcctttaca gcatcaatta
      961 tttcggcaat atcaagtgcg gtaatatcct tgaggtgttg cattccacaa tgctcacgga
     1021 aaagacggat gggtttgcct ttttgccgat aggagttggg tcttagttca ttatgttgca
     1081 gcctgtcctc ctggatagaa ttatatttat caagccattc tgtcaccgta atgtctgagc
     1141 gcctgccttt cattctttcc agacgctcat tgacgcttaa tatttgtcgg gtacgttgtt
     1201 cagcaataat ggtatttgct tcagtagcaa cttgttttgc ttcattctca tcagttccta
     1261 agctatgaaa acgaccggat agtggatgtt tgtattgcca atataccttt ccggttcgct
     1321 tatctaattt gcaatataaa ttgggtatag agattttgtg agatcggggg ctagcagcca
     1381 tcagcgatta tccgttggag ttttgggttt gcgtttattg ggagttgcgg ttctgcaagc
     1441 gttcctacaa aacgggaatt tcggtcaatc atccagtagc gaccaacttt tatagcgggt
     1501 ggggccatca ttttcccttg cgcgtatttt ttcagaactc gctcacttgg tgctaagtcc
     1561 ccaaattctt ctttagccca gtcccgtaaa gtgattagtc gagacatttg tcctcctctt
     1621 agctgctgag ggagtttgtg accgatatat ctgacatgat attaagctca tggcaggtac
     1681 atctcttgac tggtcataga gataaattta atgctgagaa atgcagtatt gaatttatca
     1741 atttttcacc agcttctggt ctttctggct gtcatgcgca aaacatatgg ctttaataaa
     1801 aaactggatt gggttagcaa cgagcaactt tccgaattaa ccgggatatt gccgcacaag
     1861 tgttctgctg caaaaagtgt tctggtaaag cgtgggattt ttattcagag cgggcggaat
     1921 accggcatta ataatgtggt cagtgaatgg tcaacattac ccgaatcagg taagaaaaat
     1981 aaagtttacc tgaaagaggt aaatttacct gaatcaggta aaaaaagttt acccaaatca
     2041 ggtaaaggcg tttacccgaa tcaggtaaac acaaaagaca aactaacaaa agacaatata
     2101 aaaccttttt cgtccgagaa ttctggcgaa tcctctgacc aaccagaaaa cgatcttcct
     2161 gtggtgaaac cggatgctgc aattcagagc ggcagcaagt gggggacagc agaagacctg
     2221 accgccgcag agtggatgtt tgacatggtg aagaccatcg cgccatcagc cagaaaaccg
     2281 aattttgcag ggtgggctaa cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac
     2341 cgcgacatgt gcgtgctgtt ccgctgggca tgccaggaca acttctggtc cggtaacgtg
     2401 ctaagtccgg ccaaactccg cgacaagtgg acccagctcg aaatcaaccg taacaagcaa
     2461 caggcaggca tgatagccag caaaccaaaa ctcgacctga caaacactga ctggatttac
     2521 ggggtggatt tatgaaaaac atcgccgcac agatggttaa ctttgaccgt gagcagatgc
     2581 gtcggatcgc caacaacatg ccggaacagt acgacgaaaa gccgcaggta cagcaggtag
     2641 cgcagatcat caacggtgtg ttcagccagt tactggcaac tttcccggcg agtctggcta
     2701 accgtgacca gaacgaactg aacgaaatcc gccgccagtg ggttctggct ttccgggaaa
     2761 acgggatcac cacaatggaa caggttaacg caggaatgcg cgtagcccgt cggcagaatc
     2821 gaccatttct gccatcaccc gggcagtttg ttgcatggtg ccgggaagaa gcatccgtta
     2881 tcgccggact gccaaacgtc agcgagctgg ttgatatggt ttacgagtat tgccggaagc
     2941 gtggcctgta tccggatgca gagtcttatc cgtggaaatc gaacgcgcat tactggttgg
     3001 ttaccaactt gtaccagaac atgcgggcca atgcgctgac tgacgcggaa ttacggcgca
     3061 aggctgccga tgaactgacc tgtatgacag cgcgaattaa ccgtggtgag acgatacctg
     3121 aaccagtaaa acaacttcct gttatgggcg gtagacctct aaatcgtgca caggctctgg
     3181 cgaagatcgc agaaattaaa gctaagttcg gactgaaagg agcaagtgta tgacgggcaa
     3241 agaggcaatt attcattacc tggggacgca taagagcttc tgtgcacagg acgttgccgc
     3301 ggtaacaggt gcaaccgtaa ccagcataaa tcaggctgcg gctaaaatgg cgcgggcagg
     3361 aatcctggtc gttgatggta aggtctggcg aacggtgtat taccggttcg ctaccagaga
     3421 agaacgggaa ggaaaggtga gcacgaatct gatttttaag gagtgtcgcc agagtgccgc
     3481 gatgaaacgg gtattgaggg tatataaaag aacatcaatg ggtacacaat gatgaaacag
     3541 gtgagttgag ttcaaactgt agtacaattc tctccagttt gaacaggaaa gaatatgtta
     3601 tgaaccctta tatttatctt ggtggtgcaa tacttgcaga ggtcattggt acaaccttaa
     3661 tgaagttttc agaaggtttt acacggttat ggccatctgt tggtacaatt atttgttatt
     3721 gtgcatcatt ctggttatta gctcagacgc tggcttatat tcctacaggg attgcttatg
     3781 ctatctggt
//
LOCUS       DAZGXS010000241         3631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGXS010000241 DAZGXS010000000
VERSION     DAZGXS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(100..1257)
                     /gene="intS"
                     /locus_tag="ACHKG5_004710"
     CDS             complement(100..1257)
                     /gene="intS"
                     /locus_tag="ACHKG5_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP0870651.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1419..1493)
                     /locus_tag="ACHKG5_004711"
     tRNA            complement(1419..1493)
                     /locus_tag="ACHKG5_004711"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1458..1460),aa:Arg,seq:cct)
     gene            complement(1569..2501)
                     /gene="yfdC"
                     /locus_tag="ACHKG5_004712"
     CDS             complement(1569..2501)
                     /gene="yfdC"
                     /locus_tag="ACHKG5_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0870652.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2795..3550
                     /gene="mlaA"
                     /locus_tag="ACHKG5_004713"
     CDS             2795..3550
                     /gene="mlaA"
                     /locus_tag="ACHKG5_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0870653.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          863 a          888 c          861 g         1019 t
ORIGIN      
        1 tgttattgtt gatggtttct tggtgtaaga aatcctgaga agtgaaaagc tttgctatat
       61 gtgcaaggct tttctttttt ataagaatcg ttgtaataac tactccacct tcccatcaag
      121 ccagtccgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
      181 aatcccacgc acagatccgc cgttggcatg tgctagttgc acttcaatag cgtcagcagg
      241 ccattcgtgc tcgttcataa tcgtgctgaa ttcatgcctg aatccgtgac cgctttccag
      301 accttcatag ccgatttgtt tgatcacaag cagtacagcg ttctcacaga ttgacttctt
      361 cttatcgttg cgcccggcaa aaacaaactc tgatactggt ttagtgatgg agcttagcgt
      421 agtgagaagt tcaacaacct ggtccgacat cggaaccaca tgaatcttgc gtcccttcat
      481 cacactggcc tcgatggtga taatcctgtt ttcaaaatcg acgttcttcc attgcatgga
      541 acgaagctct ttcgttctta gtgctgtgta gcgtaaaact ttggtcgcaa tgagagatac
      601 gatacttcct gaaaatgttg caagtgcttt gttgaatgcc gggatctggt cggcaggtaa
      661 aaacgggaag ttcttcttgc ggtatccctt catggcttca gcaaggtcag gtgccgggtt
      721 atatttagcc cttccggtga caatagcgta acggaaaacc tcgccgcatc ttctgcgtgc
      781 tttgttggct cgctccattg ccccgcgatc ttcaaatctg cggattactt ccagcagttg
      841 catcggctca atatcctgaa tttcaaggcc gccgatgata ggtaaaatgt cgtcatcaaa
      901 catttttgca agttcagtcg cataggcggc tgaccagact tgcctcttat gctcgtacca
      961 ttccttgtaa atcgcactaa aggaattgtt gttagacgaa gcctttttcg cctttaccgg
     1021 atcgatgcca accgagatgt ctttcctcgc agtccatgct ttatcccttg cctcttgcaa
     1081 agtcataagc ggatattttc cgacggtcag gattttctcc ttaccgtcaa tcttgtagcg
     1141 aagctgccat acctttttcc ctgatacagg gacataaagg tacaggccat taccatcgag
     1201 taggcggtat ggtttttctt tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg
     1261 taaaaatccg gtgggtaaaa ttattttatc cactttttac ccgtcatggc gtgcggctgt
     1321 caacgatctg acgcgaacca tgacgaactg tgaatctacg gaaggcttga tattcagggg
     1381 attttgcgga ctggtacgga tggaggcgaa ctgataaatg gtgtcccctg caggaatcga
     1441 acctgcaatt agcccttagg aggggctcgt tatatccatt taactaagag gacaatgcgg
     1501 catgagtata cccgctaatg gactgcgggg taagtacgct gccgctcgat tgcttaaacc
     1561 ctcgccattt atgccgggtt tttatcattt ttcttaatgt tttccgcacg ttctgctttt
     1621 tggcgtgctt ctgctttgcg cttgttgctc atgtcgttac ggatctgtgc atgactcatt
     1681 aacgcgaaga taaaggtgcc gccgcagatg ttccccgcta aagtaggtag tgcgaagggc
     1741 cagatgaaat cgctccagtg cagcgtaccg ttaaacacca gatagaggat ttcaacagaa
     1801 ccgacaacga tgtgagtggt gtcacccagg gcaataagcc aggtcatcaa tataatcacc
     1861 acaatctttg ccgcacccgc tgcaggaaac atccatacca tagtggcgat cagccagccg
     1921 gaaatgatcg cgttggcaaa catctcgctg ggggtgttct tcatcacatc catgccgatt
     1981 ttgacaaatg catcgcgagt ttcttcattg aagattggca tatattcaaa tgcccacgcc
     2041 gcaatacctg tcccgagaat attacccagc agcacgacgc cccataaccg cataagtaag
     2101 ccaacgttgc tcattgtcgg tttttgcatg acgggtagta ccgcagtcac ggtgttttcg
     2161 gtaaataatt gctggcgggc cataatgacg ataataaaac caaaggtata accgagattc
     2221 tccagcaaga agctgcccgg cacaccttcc agttcgacat gaaatatccc ttttgccagt
     2281 aatgaagcgc ccatcgacag acccgccgca atggctgacc acagtagtgc cattgcgtcg
     2341 cgttccagct ctttttcacc atcctggcgg atatgctcat gaattgccat cgcccgggag
     2401 gggagtcgat cttcatctat ttctattttt ttgccacgct ctttttcttc gctctcaact
     2461 tcaatttcgt cgctgtgttg atcaattttg tcgttgtcca tggtctcttc tcttgaatta
     2521 gcacgtatag ctaaagcgta gcggcttttt tgctcgcaac tggcgggagt tactttgaaa
     2581 atgtagaaaa ggccgcgttt gcctttttct gtttctatag aatcaagtag cctacagggc
     2641 ggcgattacc aggctatgat caaatcagca aatcagggcg tctggacatc agttgacgtg
     2701 ctgttacaat cgcccacacc taaacaggcg gatacggtat cgttccgtca tggatggcaa
     2761 actgcataag ccataaaaaa acagggagac atttatgaag cttcgcctgt cggcgcttgc
     2821 tctgggaact acgcttctgg tggggtgtgc gagttccggt acagatcagc aagggcgttc
     2881 tgacccgtta gaagggttca accgcaccat gtacaacttt aacttcaatg tattagaccc
     2941 gtatattgtt cgaccggtcg ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa
     3001 cggtttgagc aattttaccg gcaaccttga agaacctgcg gtgatggtta actacttctt
     3061 gcagggcgac ccttatcagg ggatggtcca ctttacccgc tttttcctga acaccatttt
     3121 ggggatgggc ggctttattg atgttgcagg gatggcgaac ccgaaactgc aacggactga
     3181 acctcaccgc ttcggtagta cgcttggtca ttatggcgtg ggttatggcc cgtacgttca
     3241 attaccgttc tacggtagct tcacgctgcg tgatgacggt ggtgatatgg cggatggtct
     3301 ttacccggtt ctttcctggc tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg
     3361 tatcgaaacc cgcgcacagc tgctggattc cgacggtctg ctgcgtcagt cgtccgatcc
     3421 ttatattatg gtgcgcgaag cgtacttcca gcgtcatgat ttcatcgcta atggcggcga
     3481 actcaaaccg caggaaaatc cgaacgcgca agcgattcag gatgatttaa aagatatcga
     3541 ttctgaataa gaaacaaata aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc
     3601 cctcaattac cacaagtaaa aggagataaa c
//
LOCUS       DAZGXS010000242         3543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGXS010000242 DAZGXS010000000
VERSION     DAZGXS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..760
                     /locus_tag="ACHKG5_004714"
     CDS             <1..760
                     /locus_tag="ACHKG5_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0870654.1"
                     /translation="GKTSDTIGKSSNAQAVLPYVVKIARGTPDFTGERKSTWFISDTV
                     IANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPE
                     NTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVK
                     GSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAATSLWKTDKPGVSLNSN
                     IINIPVMASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(842..1180)
                     /gene="rcnB"
                     /locus_tag="ACHKG5_004715"
     CDS             complement(842..1180)
                     /gene="rcnB"
                     /locus_tag="ACHKG5_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0870655.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1398..2249)
                     /gene="rcnA"
                     /locus_tag="ACHKG5_004716"
     CDS             complement(1398..2249)
                     /gene="rcnA"
                     /locus_tag="ACHKG5_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0870656.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEYQD
                     AHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATL
                     VVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVH
                     GFMGIMR"
     gene            2370..2642
                     /gene="rcnR"
                     /locus_tag="ACHKG5_004717"
     CDS             2370..2642
                     /gene="rcnR"
                     /locus_tag="ACHKG5_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0870657.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2752..3066)
                     /locus_tag="ACHKG5_004718"
     CDS             complement(2752..3066)
                     /locus_tag="ACHKG5_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP0870658.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3076..3423)
                     /locus_tag="ACHKG5_004719"
     CDS             complement(3076..3423)
                     /locus_tag="ACHKG5_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870659.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1003 a          735 c          820 g          985 t
ORIGIN      
        1 aggtaaaacc agcgacacaa ttgggaaatc cagcaatgcg caggcagtat taccttatgt
       61 ggttaaaata gccagaggca cacctgattt tactggagaa agaaaatcta cctggtttat
      121 ttcagatacc gtgattgcaa atattggcgg tgagtcatcg tcatccatcg atttttggtt
      181 gggtatttgt aaggcattga agtttaactg gtgtgtgaat tatctcacca gcaaactggc
      241 gggggataca tttacgcttg ggttaaatat ttcctattat cctaaaaata cgacctgtaa
      301 gcctgaaaac accgttataa aagtagatga tatcgccttg ttccagctca gaaatcaggg
      361 aaagattgcg gcgaacagta aggaaggaac aattacgttg aaatgtgata atcttttcgg
      421 cgacaaaaaa caagcatcgc ggaatatggt tgtatatctt tctagcagtg acttagttaa
      481 aggaagtaat actattttgc gtggtaaaac agataatggt gtagggtttg tgttggatct
      541 aacagaacca ccaaaaggga ctgaggctgc cattaaaatt tcggccaacg gcgatcaggg
      601 cgcggcgaca tcattatgga aaacagataa accaggagtt tcattaaata gcaacattat
      661 taatatacca gtcatggcca gttactatgt atatgatgaa aaaaaagtta aatctggcgc
      721 actggaagca accgcattaa tcaacgtgaa atacgattaa taacaagcaa gatgagcaag
      781 tggctaataa taagaaataa taaggtgtct gcatttcccc ctctctccga gggggctttt
      841 tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc
      901 tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatcc ggcgcgggca
      961 ggttacgcaa ctgccactgc ttaatgttgt actcatcggt acggtacatc tctggtacgg
     1021 tgtcccctgg tttgtaatgc ttaaagtcag caatgaaact gcttaactca tacttagaaa
     1081 ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca
     1141 gcaaaagcgc acccagcaac atcttatttt taatggtcat aaactctcca ctttggggat
     1201 ctgtaatatc agtcgcatat ctttcctgcc atacaatata actgacaagc gatattttgt
     1261 tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgtagcaaaa gcatcctcat
     1321 tgagcgagaa gtgagcggaa taagctgatt taagaatcat cctgggctaa aaggatagcg
     1381 aagcccaggt gattgtttta tctcataata cccataaagc catgcactcc catatacacc
     1441 ccgactaaac caattaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     1501 ccgctccagc gttttgcaac ttgttgaaca ctgattgccg ctccaacgcc tactgtaacc
     1561 agcgttagtg ccagaccgat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     1621 ttcaactgaa tgcaaatcaa cagcacggta attgctgccg ggcagggaat aaggccacct
     1681 gttaagccaa ataacaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     1741 atgtcattgg catgggctcg tgcatgggca tcctgatact cttcgttgtc gccatgttca
     1801 tgatggtgat gatgttcgtg gtcgtggtcg tggtcgtggt cgtggtcgtg gtcttgatga
     1861 tgttcgtgat cgtggtgatg atgctcatgc atattctcca gccagttgcg ttcgccgcgc
     1921 caggtacgcc agaacatcca gaaagcggtg ctaataatga tcactgcgga aatcaactgg
     1981 agccacggtt ctgctgattg agcagtaaag cgcttgctga tcaccatccc gccaaaggca
     2041 atcaaccaga ccactgcggt atgcgaaatg gttgctgcca gtccgagcat caccgcctgt
     2101 ttaatggtgc ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt
     2161 tccaggccat gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct
     2221 tgctgaagaa gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc
     2281 tgattctact cccccccagt acctgatact accccccagt agattaatag tgctatgatt
     2341 tttcatgttc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat aaacagaaac
     2401 tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa atgctcgacg
     2461 agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtggc gcggtaaacg
     2521 gtctgatgcg ggaagtgatt aaaggtcatc taacggaaca tatcgttcac cagggggatg
     2581 agctaaaacg tgaagaagat ctggatgttg ttctgaaggt gctggattct tatatcaaat
     2641 gaaagtgcct aatatggccg tagttgaggc aataaataga gggacaagtg acgttaagct
     2701 gaattagagt aaatggcgtg aggacacacg ccattcgagg ttatagtttg attaattttc
     2761 gaaaaattgc ttcacagccc agccaccatg accacctgga gttatgaatg aaacaaaacc
     2821 tttgtcatct acgctctgga tttctaccgt cattgattca tctaacacgc gacatttatt
     2881 ggatgtaatg agactcagca tagcctgttg gtcattgtcc tggatgtaac tgatcatctc
     2941 gtaaaagtca gttaactttt tacagttaaa tgtaccttct ttaagtgttg cgctgtctcc
     3001 gtgacttagt gcgaatgcat ttggcatcat tgccacaaag atgacggtaa cgagtagttt
     3061 tttcatttta gcctcttata attcagaaca ttcactgatg ttgtaagtgc cttttctacc
     3121 atcatcgttt acgagatgta gcacgaagat gatgggattg gcatcaatga aagatacaga
     3181 gacgttcccg ctttcggtgc tatacatctt tgcaatctgt ttttcaccaa tatatgagag
     3241 atcccaactc actccgttca agctaaaatg catctgtttg ccgtcaatat cgatggagga
     3301 ttgtgccgta acggttttcc cattgctgcc aatttgaccc tgctgaagag catcacattt
     3361 ataggagctt gcataagtag atagaggtag tagcagaagt gcagcaaaaa ttttactctt
     3421 catattttcc ctttgcttgg atttgggtta cctattttgg ggtagtcatt ataaaactat
     3481 taattagtaa aacgaacaag ttttcatttg tttagcaatg gtattgttta ggtttgaaat
     3541 tat
//
LOCUS       DAZGXS010000243         3467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGXS010000243 DAZGXS010000000
VERSION     DAZGXS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..898)
                     /locus_tag="ACHKG5_004720"
     CDS             complement(<1..898)
                     /locus_tag="ACHKG5_004720"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP0870660.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTG"
     gene            complement(880..1389)
                     /locus_tag="ACHKG5_004721"
     CDS             complement(880..1389)
                     /locus_tag="ACHKG5_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0870661.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(1392..2357)
                     /locus_tag="ACHKG5_004722"
     CDS             complement(1392..2357)
                     /locus_tag="ACHKG5_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFP0870662.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(2360..>3467)
                     /gene="gmd"
                     /locus_tag="ACHKG5_004723"
     CDS             complement(2360..>3467)
                     /gene="gmd"
                     /locus_tag="ACHKG5_004723"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0870663.1"
                     /translation="ALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERIDHIY
                     QDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYTADVD
                     AIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVAKLYA
                     YWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYLGNMD
                     SLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSFVGKG
                     IEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWRPEIT
                     LAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
BASE COUNT         1073 a          726 c          607 g         1061 t
ORIGIN      
        1 ctcctgtttt cgattttaga ctaatgtccc ataacgattg ccaagatcca acgtcactcc
       61 aaccaatatc aacagggcat acaacacatt tttctgtttt ttccattaca gcaaaatcaa
      121 tagattcagc aggacaatct tgaaattgtt cttttgataa tcgaataaaa tctagatcaa
      181 tgtatgagga tgaggcaacc tgttcacaaa cattgtaaat gtcaggtcta aattttctca
      241 attcctcaag ataaacagat gccttaaaca tgaatattcc actattccaa tagtgattac
      301 cagaagtcat atacaattct gcggtttcac gattaggctt ttcgacaaac ttatttacat
      361 aataaaaacc agtattttca tgcccttgaa gcggtacaga tagttcacct ctctcaatat
      421 acccataacc agtttcagca tattctggta taattccaaa cgttacaatt ttaccttgat
      481 tagcgatggg agttgcattt ttaatagcat cacagaaaac actttcttta gctataacgt
      541 ggtctgccgc aagaactaga agcaatggat cttcctgagg attacgtttt aacgcatgaa
      601 acgcagatat cgctattgct ggtgcagtat ttcgcccgca tggttctaga ataatattac
      661 catttaattt atttatttcc cttaactgtt cagccacaac aaagcgatgc tgttcatttg
      721 ttatcactaa tggtttttga catgataggc ctgaaagtcg tagcaaagtc gtttgtaaca
      781 aggtgttatc accagagagt tgtaaaaact gctttggata tagttcacga gaaagtggcc
      841 aaagacgact gcctgtacca ccggccataa ctacagcaat tattggcgca tcagacatat
      901 tatatcctta ttatctaagc caatcgcatc atttgtttta ttgttaaaat atgcgcgaca
      961 attataatgc acatcgtcat catttatcag ctttgctcgc gatagccagc aataagcatt
     1021 atgttgtgac ttcggtaatt tcaaaatgtt tggttgtaat ttaagaagga atgctataac
     1081 tatataatgc gttgaaaagt ctccccccat actattgtct tcgtagaagt gctgccagat
     1141 accataaaac ttacccacag agagaggcaa acgaattcct agttcaattt ctgtcaatcg
     1201 ttcaaaggct gtctgcaatt tttcatcttt caacacccta ccaccaggaa cgaaccaata
     1261 gccctgtgcc gggcggttga ttcgttttcc tagcaaaatt tcgccaaact cgttttccac
     1321 aatcaaatct atagaaataa gaggagtaga ccttacaatt gtggcaaagt cttgggaatg
     1381 taaaaacatt attacccccg atattgaagt tggttttcaa gaaaccagtt gtatgtattt
     1441 tcaagaccct tgtgaagggt aattttatga ttccaaccta gttgatgaag aagcgttaca
     1501 tcaagtagtt ttcgaggggc tccatcgggc tttgttgtat cgaacgtaat atgcccttta
     1561 taacctacaa cttttgctat tgtttccgca agctcacaaa tcgtgcagtc aatacctgtt
     1621 ccaatattga tatgagacaa cattacttta gtattttttt gccatatatc gtatggcatc
     1681 tccatgacat aaatgcttgc agaagccata tcatctacat gtaagaattc acgctttgga
     1741 gtaccacttc cccaaacaac aacattcgga gaattgtttt ccacagcatc atgaaagcgg
     1801 cgcaaaagcg ccggaatcac atgagaatta cttggatgaa aattgtcatt tggaccataa
     1861 agattggttg gcattactga acggtaatca cgcccaaact gacggttata agattcacat
     1921 aatttaatac ctgcaatttt tgcgatagca taaggttcat ttgttggctc aagtttccct
     1981 tgtaataatt cgtcttccat aatcggttgg tgtgctaact taggataaat acacgacgaa
     2041 ccgaggaaaa gcagtttatt tacattattt ttgtgggcag catgaatgac attcgcctct
     2101 atcattatat tctcatatat aaaatcggca ggataagaac tgttagctaa aatacctccg
     2161 acttttgctg ctgccaaata aacctggtcg attttctgtg aagaaaaaaa atccaaaaca
     2221 gcgctactat ccaacaagtt caattcatcc cgagtacgta aaaccaactc aacatcatca
     2281 cgttgtttga ggcgtcgggt aatagctgat ccaaccattc cttggtgacc agcaataaaa
     2341 atacgttgct tattcatcat cattccagag ctaagcttac agaaaaacca tgcgatttta
     2401 aaagagaatg ttttttagcg gcttcaagat ctttggcaac catttcagaa atcatttcag
     2461 caagagtaat ttctggtctc caaccaagtt tgagattagc tttgctcgga tctccaagca
     2521 aagtatcaac ttcagctggt cggaaataac gaggatcaac agcaacaatg acatcacctg
     2581 gtttcacacc tggagcatcc tgtccttcaa ccgaatctac aatgcctttt tcttcgattc
     2641 ctttaccaac aaagctcatc ttaataccaa gttgtgctgc tgccatttcg acaaactgac
     2701 ggactgagta ttggactcct gttgcaatca caaaatcttc gggttgctcc tgttgtaaca
     2761 tcaaccattg cattctaaca taatcttttg catgtcccca atctcgtaac gaatccatat
     2821 tccctaaata caaacatgat tccaagcctt gtgcaatatt tgcaagtcct cgagtaattt
     2881 tccttgttac aaacgtttct ccacggcgtg gagattcatg attgaacaat ataccattac
     2941 atgcataaat accatatgac tctcgataat ttaccgtgat ccaatatgcg taaagttttg
     3001 caactgcata aggggaacga ggataaaaag gggtggattc tttttgaggg atttcctgaa
     3061 caagtccata taattctgag gttgaagctt gatagaaacg cgttttgttt tccaatccta
     3121 aaaagcgaat tgcttccagt aaacgtaatg taccaattgc atcgacatcg gctgtatatt
     3181 ctggagactc aaaagaaact gctacgtgac tcatagcagc taaattatat acttcatctg
     3241 gctgtacctc ctttagaatt ctagtgaggt tagatgaatc agtcagatct ccatagtgca
     3301 agtgaaaatt tgggttagaa ccatgtggat cttgataaat atggtctatg cgttctgtat
     3361 taaaagatga ggctcggcgt ttgataccat gaacttcata ccctttatca agcaaaaact
     3421 cagctagata agatccatct tgtccagtta cacctgtaat aagagcg
//
LOCUS       DAZGXS010000244         3418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGXS010000244 DAZGXS010000000
VERSION     DAZGXS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..198
                     /locus_tag="ACHKG5_004724"
     CDS             <1..198
                     /locus_tag="ACHKG5_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0870664.1"
                     /translation="MLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRG
                     CELRRNVGNFGGFLSINKLSQ"
     gene            complement(215..535)
                     /locus_tag="ACHKG5_004725"
     CDS             complement(215..535)
                     /locus_tag="ACHKG5_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0870665.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            643..816
                     /locus_tag="ACHKG5_004726"
     CDS             643..816
                     /locus_tag="ACHKG5_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0870666.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            887..1810
                     /locus_tag="ACHKG5_004727"
     CDS             887..1810
                     /locus_tag="ACHKG5_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0870667.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYNYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHHY"
     gene            1865..2602
                     /locus_tag="ACHKG5_004728"
     CDS             1865..2602
                     /locus_tag="ACHKG5_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0870668.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            2599..3180
                     /locus_tag="ACHKG5_004729"
     CDS             2599..3180
                     /locus_tag="ACHKG5_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0870669.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQR"
BASE COUNT          856 a          788 c          929 g          845 t
ORIGIN      
        1 atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta taacggtcct
       61 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
      241 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      301 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      361 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      421 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      481 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      541 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatggtg gcattatggc
      601 cttcatggcg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      661 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      721 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      781 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      841 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      901 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
      961 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
     1021 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
     1081 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
     1141 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1201 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1261 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1321 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1381 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1441 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taatcggcaa
     1501 acaagagaag agcaccaacg agctttccgc aaaagaagca aacagccttg tatggctatg
     1561 ggattatgcc aaccgctcac aggcattatt ccgcgaattg tatccagcgc taaagcaaat
     1621 tcaatcgaac tattccggca gatgttacaa ctacggtcat gagttctcgt atgttattgg
     1681 aatggcgaga gacgttttaa tcaatcacac acgagatgtt gatattaatg agccagacgg
     1741 accaacgaat ctttccgcat gggtaaggct taagaacaaa gaattacctc cttcactaca
     1801 tcactactaa cagattgcca acgaaatgac ccagcttcgg ctgggttttt tatcaggagt
     1861 tctcatgctc tatagcaata tattggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1921 cggcttcgtg gtgagaacgc cggaggggga gcggtatatc ccttgtgtga atatctctgc
     1981 agagccggag gcgtattttc gtattgcacc ggaagactgg ctgcgggcag agatgcaggg
     2041 ggagattgtg gcactggtcc acagtcatcc cggtggtctg ccctggctga gcgaggccga
     2101 ccggcggctg cagataaaaa gtgcactgtc ctggtggctg gtctgccggg gggacattca
     2161 taaattccgc tgtgtgccac atctgacagg acggcgcttt gagcacgggg tgacggactg
     2221 ttacacgctg ttccgggatg cataccatct ggcgggaatt gatatgccgg attttgagcg
     2281 tgaggatgac tggtggcgta acggtcagaa cctttacctg gacaatatgg cggtcaccgg
     2341 cttttaccgg gtgcccctgt cctctgcaca ggcgggcgat atcctgctgt gctgctttgg
     2401 cgcatcggtg gccaatcatg ccgccattta ctgcggcaac ggtgaactgc ttcaccatct
     2461 gcctgaacaa ctgagtaaac gggagaggta ttccgaaaaa tggcaacgac gaacgcattc
     2521 agcctggcgt caccgccact ggcacgtatc tgccttcacg gggatttaca acgatttagc
     2581 cgccgcctca gcctgtatgt gaacacggca gcggaagcca tccgggcgct gtcgttacag
     2641 gtgccgggat tccgccgtca gatgaacgaa ggctggtacc agatacgtat tcgcggtgag
     2701 gacacggcac cggaggcggt gtacgcccgt cttcacgaac ctctgggtga gggggcggtc
     2761 atccatattg tgccgcgact ggccggagcc ggaaagggcg gactgcagat tgtgctgggg
     2821 gcggcggcca tcgtggggtc gttcttcact gccggggcat caatggcgtt atggggttca
     2881 gccctggcag ccggtggttt ttctgccacc acgatgctgt tttcactggg ggccagcatg
     2941 atactgggtg gtgtggctca gatgctggcc ccgaaggcta aagtaccgga gtacaaaagc
     3001 acggataacg gcagacagaa cacgtacttt tcctcgctgg ataacatgat tgcacagggg
     3061 aacccgatgc cggtgcctta cggtgaaatg ctggttggct cacggcgaat ctcccaggac
     3121 atcagtaccc gtgatgaagg cggtgacggg aaggtggtgg ttatcgggcg gcagaggtaa
     3181 aaagaataaa aaaatcccgc agtgaccgaa ggctgcggga acagaaaatg aagattaacc
     3241 acagggagtt ttgtttttat tggcccgaaa aaactgtaac gcccgggaat gatatctgcc
     3301 acgggggcgt acagaaaatg tgaagatatt cagaattttt attcagtcat gatacaggca
     3361 tcctccggga tgcctgttgt ttttgtgcgt aacagttatc acagtaaagg gtgagaca
//
LOCUS       DAZGXS010000245         3401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGXS010000245 DAZGXS010000000
VERSION     DAZGXS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..288
                     /locus_tag="ACHKG5_004730"
     CDS             <1..288
                     /locus_tag="ACHKG5_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP0870670.1"
                     /translation="EYEIAMLLVTKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAF
                     VAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            360..1607
                     /locus_tag="ACHKG5_004731"
     CDS             360..1607
                     /locus_tag="ACHKG5_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP0870671.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1687..1854)
                     /locus_tag="ACHKG5_004732"
     CDS             complement(1687..1854)
                     /locus_tag="ACHKG5_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0870672.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(2205..2831)
                     /locus_tag="ACHKG5_004733"
     CDS             complement(2205..2831)
                     /locus_tag="ACHKG5_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0870673.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            3134..3310
                     /locus_tag="ACHKG5_004734"
     CDS             3134..3310
                     /locus_tag="ACHKG5_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870674.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
BASE COUNT          802 a          727 c          798 g         1074 t
ORIGIN      
        1 gagtatgaga tcgccatgct gttggtgact aaaggtgcag aaggggtggt ggtctgttat
       61 cgaggacaag ttcaccattt tgctggaatg tctgtggatt gtgtcgatag cacgggggcg
      121 ggagatgcgt tcgttgccgg gttactcaca ggtctgtcct ctacgggatt atctacagat
      181 gagagagaaa tgcgacgaat tatcgatctc gctcaacgtt gcggagcgct tgcagtaacg
      241 gcgaaagggg caatgacagc gctgccatgt cgacaagaac tggaatagtg agaagtaaac
      301 ggcgaagtcg ctcttatctc taaataggac gtgaattttt taacgacagg caggtaatta
      361 tggcactgaa tattccattc agaaatgcgt actatcgttt tgcatccagt tactcatttc
      421 tcttttttat ttcctggtcg ctgtggtggt cgttatacgc tatttggctg aaaggacatc
      481 tagggttgac agggacggaa ttaggtacac tttattcggt caaccagttt accagcattc
      541 tatttatgat gttctacggc atcgttcagg ataaactcgg tctgaagaaa ccgctcatct
      601 ggtgtatgag tttcatcctg gtcttgaccg gaccgtttat gatttacgtt tatgaaccgt
      661 tactgcaaag caatttttct gtaggtctaa ttctgggggc gctctttttt ggcctggggt
      721 atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga aattttcatt
      781 tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc gcgttctttg
      841 ccggcatatt ttttagtatc agtccccata tcaacttctg gttggtctcg ctatttggcg
      901 ctgtatttat gatgatcaac atgtgtttta aagataagga tcaccagtgc gtagcggcgg
      961 atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga aacttctggg
     1021 ttttcgtcat atttattgtt gggacgtggt ctttctataa catttttgat caacaacttt
     1081 ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc ctgtatggtt
     1141 atctcaactc attccaggtg gtactcgaag cgctgtgcat ggcgattatt ccgttctttg
     1201 tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg gcgttgcgta
     1261 tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag ctgttacatg
     1321 ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca aactttgata
     1381 agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg cttgggattg
     1441 tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag acagttttct
     1501 tcgcaatttc gggtattgtc tgcctgatgt tgctatttgg cattttcttc ctgagtaaaa
     1561 aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg taaacttttt
     1621 ccggttgttg tcgatatctc catatccttc aaccggaaaa taataatact aaaatgctta
     1681 gccctgctaa taatcgccta atccaaacac ctcattcatg ttctggtaca gtcgctcaaa
     1741 tgtactccgg atacacggtt cgctgatttc caggacattg tcgtcattct gcgacctgtc
     1801 ccgtgtatca cgggcctgcg aattcatcaa ggaatgcatt gcggagtgaa gtatcgagtc
     1861 acgccgtatt tcgctatcag gattctgtgt gatggttaca tcgcccggcc ctgggctgtt
     1921 tagtcatcag cgctttctga cagtgttgag atttcaacct gttgcagtaa aaatgagtag
     1981 atatagggca agtgtgctgc caaacccatc gtttacggga tgaaggtaga tttggtttga
     2041 agggtatctg gtgtccctgc aggaatcgaa aaattagcta acaatttgaa ttgtagttta
     2101 tttctctttc gattttggtg agtgccccaa taggtgcccc caagcgttgt gatcagaaat
     2161 gctggttttt acctgatgaa acaagcctga cgacctttca aattttattc tgattttacg
     2221 atcaatagct cgttttttat ccggtagtaa gtcgctcggc tgattcctgc tgccttcatg
     2281 atgagttcag gttttactcc ttgctccaac atctctctaa cgatctctcg ggcttcctga
     2341 tttttcgccc tcccaatgta tatgccttcg cttttagctc gttcaatgcc ttgctgttgg
     2401 cgtttacggc gtgtttcgta atcctgccgt gccattgtgg caaggatatc aatcaacatg
     2461 gcatttacgg ctctcaggat gccgctggtt agatcattgc ctgccatctc gaccatctgc
     2521 cagctcgttg gtaaatccat tgatacgatg attaaccctt ttcggttgag cgtatctttc
     2581 agttcgaccc atgcactatg ttctaggcga ctcagacgat ctatagcttc cactagcaac
     2641 agatcgccgg actgggcttc agacaatagc cgcataagct ctacgcgctc aagcttcgcc
     2701 ccgctagcat tttctatata ctctcctgca atctgccagc cttttgattt tgaaaattca
     2761 cgtaggctgg taagtgctcg atcagcgtgt tgcccctcag ttgatgctcg gcagtaaatt
     2821 ctaacgttca ttgcattgtc tcaaaagggt tgtgaatata agtgcaaatg atacataatg
     2881 cagtttgtgt agtcaaaatg ttcctataca accctattga gactttgtat caccttgtca
     2941 ttacggtata cccaattgag acaatgataa cggctggtgt ttaaggtgat atattgagct
     3001 agccgaaaga aacagcccga caccgttttt accgtatgag ataatttgtt gtgctggtgg
     3061 ggttgcttgc cagtacttgg aatggtacaa atctgtacca ttgttcgacg gtctattaac
     3121 aaggagtgtt aagatggaaa aagacctgaa agagttgcgg gaatacctat tactttcccc
     3181 cgtctattct gaaattaacg attgtctggc tagtctctca atggaagacg tgaccaatga
     3241 agagttcgcg gaacgtttgg gaattgctcc tgattggatg gatgctatag atactagcca
     3301 acttgcttaa gaaaggatgc gccattgctg ggcgcgtttc tcatttctcc cgtagcacta
     3361 cgcatacctt ctaataaatt tcaccatcaa aatcagtaga t
//
LOCUS       DAZGXS010000246         3386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGXS010000246 DAZGXS010000000
VERSION     DAZGXS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            241..915
                     /gene="tnpA"
                     /locus_tag="ACHKG5_004735"
     CDS             241..915
                     /gene="tnpA"
                     /locus_tag="ACHKG5_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP0870675.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            912..1259
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004736"
     CDS             912..1259
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0870676.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1279..2649
                     /locus_tag="ACHKG5_004737"
                     /pseudo
     CDS             1279..2649
                     /locus_tag="ACHKG5_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2654..3382)
                     /locus_tag="ACHKG5_004738"
                     /pseudo
     CDS             complement(2654..3382)
                     /locus_tag="ACHKG5_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001413889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
BASE COUNT          858 a          921 c          887 g          720 t
ORIGIN      
        1 gtgcaatagt catccgtcgt tgacaacagc tgatactgag ggtttccggg tcctctgcga
       61 gcgattctgg ctgttcggca gctgcatcga gagtcacagg aagtagctgg gggctctctg
      121 aagaaggtaa tagcagcttt ccctcgcgcc attgttgtgt aagcgcccca tctgcgacgt
      181 cttgtgaaaa ttgtcctgtc tggcaacaat cgcgcccatc tatatagatg gacacgaacg
      241 atgaattccc agacaaaaaa agatattccc tgcttccgtt cttatttgcc tgatgctctg
      301 cgtttaagat ttgaagataa actgaccatc cgggccatcg ctcagcgtct aggtctcagt
      361 cattccacaa tacatacgct ttttcagcga tttattgcat ccggtatcgc atggccattg
      421 cccgattcag tttcattagc tcagcttgac gccatccttt atgccaacag aaagaaggaa
      481 ttaacagagc ctcaaatcag cgaaggcaca tggcgaaaag aacgacgaac ctcctacagt
      541 cgtgaattta aaatcaggct ggtaaaacag gcattacagc ccggtgctgt tgttgcccgg
      601 atcgccagag agcacggtat caatgataac ctgctgttaa aatggaaaag ccagtacgag
      661 gacggcttac tgagcgatga tgacatacag gaatgcatgc ctgtcccggt ggctctgact
      721 gatacgccag agccgaccag accagttaca aatcccttct ggcgtaacaa gcctgatgag
      781 tgccctgaga gtgatcccgg aaacgtccca cggtgcgagc tgcatcttaa atcaggtgtg
      841 gtaaaactgt ttgaccctct cactccggaa atgttacggg cgctaatccg cgaaatgaaa
      901 ggaggtaccc gatgataacg ctgccaaccg gtaccagaat ctggatcatc gctggcatca
      961 cagatatgcg ttgtggcttc aacggcctgg cttcgaaggt gcagaacacg ctgaaagatg
     1021 ccccgttctc cgggcatatc ttcgtcttcc ggggccgcag tggcaaaatg gtgaaaatac
     1081 tgtgggccga tcgtgacggg ttatgcctgt tcgccaaacg cctggaacgg ggccgcttcg
     1141 tctggccggt aacccgggaa gggaaagtgc acctgacgcc agctcagtta tccatgctac
     1201 tggaggggat cgcatggcaa catcccaaac ggacagaacg gcctggcatc cggatataac
     1261 ccgtgataaa acaggggaat gaacaacaca ctccccgaca acatcgagca actgaaggcc
     1321 ctgctgatcg cacagcaggc tgttatcgtc cgtctgtctg gtgaaataac cggctatgcc
     1381 cgcgagatca gctcactcag agcgctggtc gctaaactgc agagaatgtt gttcggtcgc
     1441 agcagcgaga aaagccgcga gaagatagaa aagaagatcg cacgggcaga aacgcgtata
     1501 accgagctcc agaacaggct tggtgaggcg cagttgcaac tcacctcaat ggccggagag
     1561 acagcgccga aaacatcaga ctctcccgtc cgcaaagcac ttccggcaac acttccccgt
     1621 gacaggcagg ttatctcccc ggcagaaacc gaatgccccg tctgcagcgg taaactgaaa
     1681 ccgctgggag aaagcatctc tgaacaactg gatatcatca acaccgcgtt cagggtaatc
     1741 gaaacggttc gcccaaaacg ggcctgcagc cggtgcgact gtatagttca ggctccgcag
     1801 ccaccaaaac ccatcgagcg cagttacgcc agtccggctc tgctggcccg cataatcatg
     1861 gctaagttcg ccgagcatct gccgctgtac cgtcagtcgg aaatctatgc ccgccagggc
     1921 gtggagctgc accgcaatac gatggggcgc tgggttgaca tcatgggaga gcagcttcgc
     1981 ccgctgtatg atgaactgaa gcactatgtg ctgatgccgg gtaaagtgca tgccgatgac
     2041 acgccggtaa atgtactgga gccgggtcag ggtaaaaccc gtaccggacg gctgtgggtc
     2101 tatgttcgtg acgatcgcaa cgccggttcg accatgccgg cagcggtgtg gttctcatac
     2161 tctcccgacc gcaaaggcat ccacccacag caacatctgg cggactacag aggtatcctg
     2221 caggccgatg catatgcggg ttacaatgct ctttacgaaa gcggtcaggc aaccgaagcg
     2281 gcttgtatgg cacatgcccg acgcaagatc cacgatgtac atgtccgcca tccaacgaca
     2341 gtaacgggag aagcgctccg tcgtatcggg gaactgtacg ctatcgaggc tgagatccgc
     2401 ggcagtccgg cagaagagcg actggcggtc agaaaagcca gaacggtacc gctaatgcag
     2461 tcgttgtatg agtggctcca ggggcagatg aacacgctgt cgcgccactc ggatacagcg
     2521 aaagcgttca cctatctgct gaagcaatgg gacgctctga acgaatactg ccgcaatggc
     2581 tgggtggaga tcgacaataa cctgtgtgaa aacgccctcc gggtagttgc actggggcgg
     2641 cgtaactacc ggacgtggtt tctgccaggt aaaggcaacg gaacaaaaga atcatggttt
     2701 ttccggaacc ggccacacca tgaatgacac gatgcccgtc accaatattt ctggccagaa
     2761 tctcctgctg aatatccata attttgattt gtgagttctg tttcacggtc acttcaggaa
     2821 acagatgggc gcgcagaata tccctcatcc ggggtgttac ccgggttctg aaccccgtgg
     2881 taaacatccc ggcaattttt tgccggaata cctcaggtaa aacagactgc gtcatttcat
     2941 cctgacagat tgtctgtgct gaaggaaaaa tcttttccac cgcattctca ttattccctg
     3001 tcagtgcctt cagttgctga cgggtaatgc gggtaaacac cacaccatac gcccaggcca
     3061 ggttcagccg ccccttatac tgaccgtcat tctgccgtaa gcacgggtca gcaggtaagg
     3121 cattaaccgt atcacaggca taacgacgaa cctgtaccag cggatttatt tctgatttca
     3181 gaagaccgtc ggtttccagt gtaacctgct cctgatttgc tttacgtaat gtggatacag
     3241 tccagtcctt gacctccaga aataccagcc cgttatccgg gtcaataatg acaaaatccg
     3301 gatgtcggtt tttccgtccc accgggatat cgtaccagac aaagcaatca tcattcagaa
     3361 agctctccag tcgtgaagcc accctc
//
LOCUS       DAZGXS010000247         3355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGXS010000247 DAZGXS010000000
VERSION     DAZGXS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..453
                     /locus_tag="ACHKG5_004739"
                     /pseudo
     CDS             1..453
                     /locus_tag="ACHKG5_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            661..1017
                     /locus_tag="ACHKG5_004740"
     CDS             661..1017
                     /locus_tag="ACHKG5_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870677.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(1305..2231)
                     /locus_tag="ACHKG5_004741"
     CDS             complement(1305..2231)
                     /locus_tag="ACHKG5_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0870678.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKRSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            2388..3308
                     /locus_tag="ACHKG5_004742"
     CDS             2388..3308
                     /locus_tag="ACHKG5_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0870679.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          977 a          760 c          698 g          920 t
ORIGIN      
        1 aacaccggtt atcgactgat tgacaccgcc gcctcctatc aaaatgaacc ccaggtcggg
       61 aacgcactga aacaaagcgg tatcgcccgt aacgaactct ttgtcaccac gaaactctgg
      121 ttgcaggata ccagttacga aggggccaaa gcccagttcg aacgctctct gaatcgactg
      181 caacttgatt acgttgacct gtatttgatt catcagcctt acggtgatgt ccacggtgcc
      241 tggcgtgtca tggaagagct gcaacaggcc ggtaaaattc gcgctattgg cgtcagtaac
      301 ttccatcctg accgactggc tgaccttatc gcctttaata acgtcgttcc agcggtaaac
      361 caggttgaag ttaacccttt caatcagcag ctacacgccg ttccgtggat gcaaagccgt
      421 ggtattcagc cagaagcctg ggcaccgttt gcagttacag ggcggtaagc gattatgcat
      481 ctacctattt acgtcggtca ctcagtcaga gtcttgccca tattcaatga ggttcaacac
      541 cgcagccaat gaaaagtagg ccatgcgggg attaatagca ttactcatta accaatcgct
      601 cttttcattt acgtgctttt tatgtaacat attgctttta ttgatcttcc tggagtttgc
      661 atgaataaga atctcatcct cgcatttgca ttattttcct tacctgtatt tgcagaagaa
      721 gatctggggc caggcaaata tgtttgtgac attaggattt ccagcctgga taccgctact
      781 caaattctgt caaaatccgc aacagttctc gataatggaa acaatttcat cgttcaaatg
      841 ccaaatggag atcaattgta ctctccagat ctggaaaatg ttgatgatgg tataaaacaa
      901 aaagcaacaa taggtggagt gacatttatt cgtcgtccaa cttttaacga tcgcttcatc
      961 gtggaagatg gaaatacagg attcttctat aagatgcgaa actgcgagaa gaaataaagg
     1021 aagtttgata gcgatatcct cctgatttca ggctttgtac ctgttcaaga cttacaacta
     1081 caagccgcta cgtgttaacg cctcccaaaa taaccaccgt taaccgtagc ggtcatagat
     1141 actattattt taaccacatt actggtgata atgataattg atattatggc cgtatagaaa
     1201 ataagtatta attatcagaa actttattat gtaaacgcat cctaatgatt acgttattcc
     1261 tcttaccgat attcgcccag agcaaccatc aattacacat atcatcaatt ttcgagtttt
     1321 actcttttct ccaccataaa atcaataaaa gcacgaactt tagagggcat tctcacccgt
     1381 gatgaaacat aaatataaaa cccaggaaat gaaacagccc aatcctcaag aatcggctgt
     1441 aacttgcccg cattgatatg ttcagcgatt gcaaaatcca gatgttgaat gatacccgtt
     1501 ccattcagcg ccattcgtat catgctttcg tcatcattag aaatatactt acctgaaacc
     1561 tctatttcga actcttcatt accccccaca accttagaaa actcccatgc agagagtgca
     1621 ccgctacgtt tatgccgcaa ccgaaggcaa ttatggttac tcagttcctg aggcgtttca
     1681 ggaataccgt gttcttttaa ataatctggc gatgccacag taaccagttt aataagcgga
     1741 gaaacaggaa cggcagtcat atgctcatcc agtccttgct caagacgtat gcccacatca
     1801 cagccgtccg caataatgtt agataaaccg tcatccataa tgagttctat ctttgttttg
     1861 gggtaacgag taagaaactc gccaatatga ggctcaacaa gcatccgtgc tgcaacacgg
     1921 gaggaattta ttctcaacag gcctgtaggt tcaagttggg tatcaccaac atctttcaaa
     1981 gcatcatcaa tttgccccaa agccgacacc agcacttcat acagacgctg tccttcctca
     2041 gtaagcgaga tattgcgggt cgtacggtat atcagcttgg tgttaagacg ttcctcaaga
     2101 gattttaaat tctgggacaa tgcggccagc gtaataccca tttcagaagc ggctctggaa
     2161 aaactgccgg cacgaacaat cagcgcaaac caggcaagag aaggcaaaat tgaaggctct
     2221 attttcatca ttgtaaacct taacttaaca ctgtgataga tattagacca ttgatgataa
     2281 ccaatacaag tcatactatt acgccgttct cgatatgaac acaaatgcca gttgcggata
     2341 atagaaatga tatttaaatc tgtttaggca aatccccgga gcttgaaatg aaaactgttt
     2401 cgataaaaca cacttactgg gatatagctg ctgatatcta tttccctgac gattttaata
     2461 gtgaaaaaaa gtatcctgcc attatcagcg cccatccaat tggaagctgc aaagaacaga
     2521 catcgggtag tgtgtatggt gccgccctgg caaaagctgg ctttatcgtc attgcttttg
     2581 atgctagttt tcagggaagc agcggtgggg aaccgcgtta tctggaagat ccaacaatgc
     2641 gagtaaaaga cttcagcatt gtcgttgatt accttaccag cttaccttac gttgatgccg
     2701 ggcgtattgg tgtactgggt atttgtggtg gaggtggata tgccattaat gccgcaatga
     2761 cagaacgccg aattaaagct attggtactg tcacgggtgc aaactacggg cgtcttatgc
     2821 gtgagggatt tacagcgttt aatcctatcg gagcacttga agcaatggcg caacaaagaa
     2881 cagacgaagc taacggcgca aaacttcgcg tagatgattt actcccctct tctccggatg
     2941 cagctcttga agcgggacta acagaaatag acctttttga agccacagaa tattatcgtt
     3001 cgccacgagg ctatgcacct aatggggtaa atcgttcgct attctcacat cagactgttg
     3061 ccgtgggttg ggatgcattt catcttgcag aagtgctgtt aactcaacca ataatggttg
     3121 tggtgggcga tagagtgggc gcatttggtg cctaccgtga cggctgcgaa attattggcc
     3181 gcgctgcttc aaaacataaa gagctggtcg tggttgaagg ttattcacat tacgacctgt
     3241 atgataagcc agaacctgtg aaacaggcac tcgataaatt aattcctttt tataaaacac
     3301 atttgtaata attttacagg gagattcctt atctccctga catttatcct ttatt
//
LOCUS       DAZGXS010000248         3341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGXS010000248 DAZGXS010000000
VERSION     DAZGXS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..910
                     /locus_tag="ACHKG5_004743"
     CDS             <1..910
                     /locus_tag="ACHKG5_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFP0870680.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="ACHKG5_004744"
                     /pseudo
     CDS             919..1071
                     /locus_tag="ACHKG5_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="ACHKG5_004745"
     CDS             1161..1535
                     /locus_tag="ACHKG5_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0870681.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..2023
                     /locus_tag="ACHKG5_004746"
     CDS             1532..2023
                     /locus_tag="ACHKG5_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0870682.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            2035..2232
                     /locus_tag="ACHKG5_004747"
     CDS             2035..2232
                     /locus_tag="ACHKG5_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0870683.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFNNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            2317..2658
                     /locus_tag="ACHKG5_004748"
     CDS             2317..2658
                     /locus_tag="ACHKG5_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870684.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            2719..2862
                     /locus_tag="ACHKG5_004749"
     CDS             2719..2862
                     /locus_tag="ACHKG5_004749"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870685.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            2856..3275
                     /locus_tag="ACHKG5_004750"
                     /pseudo
     CDS             2856..3275
                     /locus_tag="ACHKG5_004750"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          889 a          864 c          829 g          759 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt actgccctta ccggcatacg
     1741 ctgcagtacc gcacatatca cggaaacgga caacgcctgg ctgtactcgc tgtcacacca
     1801 gaccagtgac ttcggtgaat cagaatggat tcattttacc ggtaacggct atctgttgcg
     1861 taccgatgcg tggtcgtacc cggttctgcg gcttaaacgc ctgggtctgt caaaaacgtt
     1921 ccgtcgtctg gtcgtcacac tcatccggcg ttatggtgtc agtctcattc atctggatgc
     1981 cagtgccgga tgtctgccag gtttacccac ttttaattgg taactaggaa caacatgaca
     2041 tcattaaccc cggaagcagc actggatatt ctgattgcgt ggctgcagga caatatcgac
     2101 agcgaatccg gaattatctt caacaacgat gaggataaaa cggattcggc agcattgttg
     2161 ccctgtatcg aacaggtcag ggaagatgtc cgtaccctgc gccaactgca gcttctgcaa
     2221 cagaaccggt gagtctcact caccatactt cattccactg acgccagcct gaacagggct
     2281 ggcgttttcg tttataaacc aaaaggaaaa ccgataatgt caggcatcat aatctcccgt
     2341 ccggaagtgg acaccgggca tacggacgtt atctgctcca ccagtatccg acacgttgtg
     2401 acggtacgga atgcagcact gcaacaaacc gaaacgctta tccggcaact ggcagaaatc
     2461 tcagtcctga cagcagcaga cattggcggc aaaaccgccc tggaccgggc gatgaaacag
     2521 gattttcact gcggctgctg gctgatggag aaacctgaaa ccgcaatgaa agccatcacc
     2581 cgcaacctcg actgcgaaat ctggcgtgac ctgatgcaac gctccgggat gctgagtctg
     2641 atggatgcac agggctagga tacatggtac cgctcactgg agtacgataa tattccggaa
     2701 atcagcgagg cgaatatcat gaatgcattc aaacaactgc accagagtaa ggatgaagtg
     2761 tttgagcgtg gggtgatcaa cgtcttcaga gggccgaacg gaattacaaa accaattccc
     2821 cctgtaaatt cggcagtaaa attatcgtca acaacttggt gagatgggac agatggagat
     2881 ttcatcttat cagtggaatg caggcagatc gcttagctga cctggaaaga atgttgcatc
     2941 tgctcagcgg taaaccgctc cccaacaacc gagggaatat caccattaat ctggatgacc
     3001 acctacagtc cgttcagggt aaagagcgct atgaagacaa gtaaatactt taagaagggc
     3061 ccgcacacat cacattcaga aagccagagc tggttgacag gcttaatgat attattgcca
     3121 gacactacct aggaatgtta ccttcacaaa aatctctaag aattgattca attgtgtatt
     3181 ttagcctcat tccctcatgt aaagtcattt gggggttatt atgggggtca atcatttatc
     3241 caacacgcat taatcattta aaatcaatgc attaataatc atattcgact cctgtgatct
     3301 ccgccaaact tcctcttctg aactctcccg acgtcaacaa a
//
LOCUS       DAZGXS010000249         3328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGXS010000249 DAZGXS010000000
VERSION     DAZGXS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(639..836)
                     /locus_tag="ACHKG5_004751"
     CDS             complement(639..836)
                     /locus_tag="ACHKG5_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870686.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(902..1172)
                     /locus_tag="ACHKG5_004752"
                     /pseudo
     CDS             complement(902..1172)
                     /locus_tag="ACHKG5_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1401..1673)
                     /locus_tag="ACHKG5_004753"
     CDS             complement(1401..1673)
                     /locus_tag="ACHKG5_004753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870687.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            1993..2187
                     /locus_tag="ACHKG5_004754"
     CDS             1993..2187
                     /locus_tag="ACHKG5_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870688.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2530..2658
                     /locus_tag="ACHKG5_004755"
     CDS             2530..2658
                     /locus_tag="ACHKG5_004755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870689.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2968..3303)
                     /locus_tag="ACHKG5_004756"
     CDS             complement(2968..3303)
                     /locus_tag="ACHKG5_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870690.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          984 a          760 c          732 g          852 t
ORIGIN      
        1 taaccaagta tattatcttc tggtagagat tcaggataca gtttgtagga gctttctttt
       61 ttgaacaatg atttgcagga cagttatgct aaactagcgg aatcagtatc acaacaataa
      121 atgactgatc atacctgaaa tttgaattaa caggcatcat gaatgtcaat agtctaagcg
      181 gtgactactt atatcgcttg cataaatact aaacactgac tagaaaagta ttggataacc
      241 ccgtcactaa aagtaacgct tcggttattt gctcggaatg tattaacgcc aagattgtta
      301 gccgttacgc cttatatcac tggcgctgac aaatgtttct tttctaattt actgaaccgg
      361 cgcagacacg caggaaagca cgccggcacc ggtgtaatgc cagttctaat caataagaat
      421 ggaatgacga atcagtcagg aagggataac aacccgctgc tgaacggaag gtatcagcag
      481 gaagatgaac gggaatatcg ccagcaccaa gggcagggcc gggttccaca ataccagcgc
      541 tggcattttg cacattgcca gcgtaccggc attacaacac gtttcatgct gctgcccccc
      601 aggattcctc gattcgttgc tacatccact gctctacttc acttccactg ctccacttca
      661 cttttgtacc agcggctgct gcgccccagt ttgatgggtt tagggaaatg gccttcactg
      721 atcaacttgt aaaaccattt gtcggtcata ccggtgaaac tggtgatata gttcatgtcg
      781 atcagggaat tatcggcacg cagaaatacg ctgtcgtggc ttgttgtagt caacatggtc
      841 agaatgtcct gtgagattta tgaagattgt gcgaatgcgg gaaatctact gagctgtgct
      901 ttcagaactg gcctgttacg ggataccagg gattaccggc ggggtaacgg gcttccggat
      961 catacacacc acgattatcg cggacaaaat cactgaacgc ccatatcacc tctttaagta
     1021 tgtcttcgca gtccggtaaa tgacgatcca gcgccacatc ccgggtggta cagctttgat
     1081 gcgcccggtg acacaaagcc cggattgttc cagacatcct gaatcaaacg ccccagatta
     1141 ggggcgtcga aatatgcctc tctgaccatt atattccggc gtataggtag caggtctgaa
     1201 gtgacaatgc gtcacctgac gttaaaagtc acccaagatg accccagtgg gggcaacaag
     1261 tataaaaccg ttttttgatt aagtggtcgg atatgaaaag gggaaggttt atgtgaaaaa
     1321 tacctgtgtt ttctttgaaa acacacaaca gtatcaggtt atttatgttt atacagtgcg
     1381 ttccaaactg acctgaataa ttacgcaagt tgcaacaata aaacattttt gccgtagtgt
     1441 gaggtactcc ggtaaattgc ttcccaggtg cagacaatga ctaacgaaga actacaggca
     1501 atcgcattat ccatgcttga gcgccagcgc gaaaaagtac accagcaacg agagtctttg
     1561 cttaacccat ctccattcac caggccaaaa cgattggagc ttgaagcatt cttggacaaa
     1621 tacccacggc ggcttaaatc aggcaaaacc agaccaccag gctgatagct cacagtttgt
     1681 tctccgattc aataaaacgg ataagaacca tcagcgcggt agcgtaaggc actacagcgc
     1741 attgctttac gagtaataac aatggattta tcaccagaag acgcaaagtt tttacgagaa
     1801 ctgcaacacc gtatgctcag agaagccatt atcgaacagc aaacgggcat gagcgccata
     1861 gacccattgc gatacaaacg ggcctcatct cctttagttg acgacaattc caatgcggat
     1921 aaaacacgtc caccacactg attacatttt tcattagcac agtggtgtga tgcagcatac
     1981 gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg agcaactgtt acaggcacgc
     2041 caacgggagt ttgccactaa atggggacgt gccgcgctgc cacatgaacg cctaacctat
     2101 tttccacttg aagacctcat taaaaataat ggcaaaaaaa tcaggtctga taagaaacac
     2161 ttcttagaat gcaatgaaga caaataacaa caatctgcat attgccacca accacagatg
     2221 tggcatcgtt atcagcgcac ttgattttct ttatatccac tgacgagttt gccggtcatt
     2281 ctggtatcat gcagatattt acggcgtaca agcacactag catgacattg taataaggcc
     2341 tgagcctaac gctttaatgt gtgtcataca ctcagtggcg taatcgttga caatatggtg
     2401 catgtaatcg taagcgtcca gcgaaatcca tctggtcata atctgaagca tccgacaaag
     2461 tggtgtccac caaataagtg atctgcgccc aatatcctga gccggaactt tgacaaaggc
     2521 caacgaaaaa tgggagcgga tgttacagag ttctcggtac aggggaaaag tggtacctgt
     2581 cgccagttct ccgatctgtt taaccgggaa attatctcct acagcttgtc agaaaggtcg
     2641 gtgatggagc atcgttaata cgatgctata cgatgcattc acagtgcccg gccagaggat
     2701 gccccgtcgc tgcatatgga tcagggttgg caatatcgaa ttgcaggcta taggcaaagt
     2761 taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg aataacgcag
     2821 tgatgaagaa cttcttcagc acactgctaa aacgcatagt gatcgagcgc tgattctggc
     2881 gaacaactga actaatacat cagaatctgc attatgttaa ataaatataa aaagatggtt
     2941 taaatacccc gttacttttg acttacacta tacggtatcg catcgtttaa tattcgcacc
     3001 ggccagattt ttatttctat tagttgtcac aatactgaat gcgtacgacc acagtattct
     3061 ggctcctgtg tggttatgct ttaattctgc gttccgggca gataagcagt tgcttgcagg
     3121 aatccttctt gtgttaatgt cagttcccct tttaccagtg ctgatttcca cattccgtcc
     3181 aacagagctt atagcctttc cctggattat agcattgtcc ggctgaagtt ctttttgaat
     3241 aataatagaa gcactgctgg cagatccagt ccgtttttca taacccactg tactgataac
     3301 cataatctaa tcagtagaaa ttgagtcg
//
LOCUS       DAZGXS010000250         3305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGXS010000250 DAZGXS010000000
VERSION     DAZGXS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            6..81
                     /locus_tag="ACHKG5_004757"
     tRNA            6..81
                     /locus_tag="ACHKG5_004757"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKG5_004758"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKG5_004758"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /locus_tag="ACHKG5_004759"
     CDS             complement(467..1225)
                     /locus_tag="ACHKG5_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0870691.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKG5_004760"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKG5_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0870692.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..>3305)
                     /locus_tag="ACHKG5_004761"
     CDS             complement(2346..>3305)
                     /locus_tag="ACHKG5_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0870693.1"
                     /translation="QQGDTYGRVFIVGLLNTLLVSALCIVFASVLGFFIGLARLSDNW
                     LLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIPSP
                     QLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQWL
                     FGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQH
                     EAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLFAG
                     TVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          908 a          872 c          815 g          710 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctg
//
LOCUS       DAZGXS010000251         3277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGXS010000251 DAZGXS010000000
VERSION     DAZGXS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..702
                     /locus_tag="ACHKG5_004762"
     CDS             <1..702
                     /locus_tag="ACHKG5_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0870694.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
     gene            801..1814
                     /gene="ybjS"
                     /locus_tag="ACHKG5_004763"
     CDS             801..1814
                     /gene="ybjS"
                     /locus_tag="ACHKG5_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0870695.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1811..2641)
                     /gene="amiD"
                     /locus_tag="ACHKG5_004764"
     CDS             complement(1811..2641)
                     /gene="amiD"
                     /locus_tag="ACHKG5_004764"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0870696.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2638..2961)
                     /gene="ybjQ"
                     /locus_tag="ACHKG5_004765"
     CDS             complement(2638..2961)
                     /gene="ybjQ"
                     /locus_tag="ACHKG5_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0870697.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3172..>3277)
                     /locus_tag="ACHKG5_004766"
     CDS             complement(3172..>3277)
                     /locus_tag="ACHKG5_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870698.1"
                     /translation="AAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          750 a          897 c          845 g          785 t
ORIGIN      
        1 gtgtggtttc tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag
       61 gggctgaaac acgatctgct ggcggatgac accgcgctac gtgcgctcat cccacaacgg
      121 ctgatcgctt tcgatgacgc ggtacgtcgc accctgaaag aagaagaaaa gctggtcaac
      181 tccagcgact ggggatacga cactcaggcc tttgcccgct ggcgaccaga gtatggttat
      241 tttgccaaac aggcgggatt taccgttaaa acgtccgcca gccttgcggc tttatggcag
      301 gtggtgaacc aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca
      361 cgggcgttga tggaccgtgc gatcggtcat aaattagcga aaggccgtcc ggagcgcgaa
      421 tatttgcaaa ctggcgatgc ggtggatagc tggaaagtga ttgtcgttga accggaaaaa
      481 caacttacgt tgttatttgg catgaaagca ccggggctgg gacgactgtg ttttaccctg
      541 gaagataaag gcgactatcg tactatcgat gtccgcgctt tctggcatcc gcacggtatg
      601 ccggggctgt tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg
      661 gcaaaacgaa tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta
      721 ttctttcatg tttcccattg catcaagggg gaaatcacgg aagaatgcgc acgtcatttt
      781 ctcaacaaca gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc
      841 gaaacgcggt agagttttta tgccagaaag gcatcagcgt gcgagcgacc ggtcgcaacg
      901 aggcaatggg taaattgctg gaaaaaatgg gcgcagagtt tgttccggcg gatctgaccg
      961 agctggtctc atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct
     1021 ccagctttac ctctccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca
     1081 ctcgccgcct aggtgaatgg gctgtcgcct ggggtgtacg taactttatt catatctctt
     1141 ctccctccct gtacttcgat tatcaccacc atcgcgatat taaagaagat tttcgccctc
     1201 accgcttcgc caacgagttt gcccgcagca aagcggccag cgaagaagtg atcaatatgc
     1261 tttcgcaggc gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac
     1321 cgcacgataa agtctttatc ccccgtcttg cgcatatgat gcaccactac ggcagcattc
     1381 tgttaccgca tggcggcagt gcgctggtgg atatgaccta ctatgaaaat gccgtgcacg
     1441 ccatgtggct ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca
     1501 tcaccaacgg cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga
     1561 atattgactg tcgtattcgt tccgtccctt acccgatgct ggatatgatc gcccgcagca
     1621 tggagcgttt aggccgcaag tcagcaaaag agccgccgct gacccactac ggcgtctcga
     1681 agcttaattt tgactttacg ctggatatta cgcgggcaca ggaagagtta ggttatcagc
     1741 cggtcatcac cctggatgaa ggtatcgaga aaactgccgc ctggctgcgc gaccacggaa
     1801 aactgccgcg ctaatcctgc ccgtatttct ccagcaacgc ttcggcaatc gcctgagttt
     1861 ctgcatccgc ttcgccgtta tataacgtcg ggcggaaatg catctggaac gccataatca
     1921 cgcgccgctg ctcgcgcggt gtcatatcag gtttaacgtc ataaccgtag cgcgccaaaa
     1981 gctccagcaa tgacgcggta tctaccggtg tgtgcggcgc gcgcccggca aggtaaaagt
     2041 tcacccgctg cgcatccggc caggcaccaa tcccctgctg cgccagttgc tgccagggaa
     2101 ataatggccc cggatcgtct ttgcgctgcg gggcgatatc cgcatgtgcc actacgtttt
     2161 ccggcttgat gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct
     2221 gtgccggttc aaacggggca aaatatttca cccccgccga tttttgccag ccacggtttt
     2281 ccagctcaat gccaatagag gtgtcgttaa ggcgcgttgc cccgcgccag gcgctaatcc
     2341 ccgcatgcca ggccagttct tgttccggca ccaattgcca gatgcgcggt ttaccgttgt
     2401 atcgcggtgg taccgcaggg accagataat gcgagctgac ctgcttatcg gtcagtgtcg
     2461 ccagcgagct atcaaaatca tctgcggtgt agtggatcac cagcacttta atgcgcggat
     2521 acgccgcctg cgcctggcgt cgggtatcaa gctgatatcc ctctttctcg acaatgcctt
     2581 tttcgcctgc gcaccctgcc agtaacagag cgacagcgat cagccagaag actcttctca
     2641 tcgacgcgtt ttcaccgccg taccgctgac gctaaccatc agcatactgc cgttttgccc
     2701 gaccgtttcg tagtcgatat caataccgac tacggcatcg gcccccagcg cccgcgcctg
     2761 ggagccaagc tcctcaaagg cgatctcccg cgctttacgt agctcttttt cgtaggcacc
     2821 ggagcgtccg ccaacgatat cgcgaatacc agcaaagaaa tcacggaaaa tattcgcgcc
     2881 taaaatcgct tcgccagtca ccacaccgca atattcaacg atggtctggc cttccagagt
     2941 tggagttgtg gaaaattgca tgatcgatct ccttgtttgt taagccttcg atctctaaag
     3001 cattatcgga ctgatatgcc atgttgggaa gaaggttttg atcagtttag gaaatcatct
     3061 cattgtgtca aaaacagcta cataagcctg tacaattatc tgtttcgtaa ccggatattg
     3121 gtttcattat ggagcaccgc ttacctgccc tattatgttg tgccggcaac attaatgagt
     3181 gggaagagtc aacttatatt gtaaatgatt cctaaagtct tccccatctt gcgcctgagg
     3241 tatataccgc tggattgctt tttggtccgc cgccgca
//
LOCUS       DAZGXS010000252         3222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGXS010000252 DAZGXS010000000
VERSION     DAZGXS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1721)
                     /locus_tag="ACHKG5_004767"
     CDS             complement(<1..1721)
                     /locus_tag="ACHKG5_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0870699.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQVAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEY
                     KRQLAELG"
     gene            complement(1771..2073)
                     /locus_tag="ACHKG5_004768"
                     /pseudo
     CDS             complement(1771..2073)
                     /locus_tag="ACHKG5_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(2100..2528)
                     /locus_tag="ACHKG5_004769"
     CDS             complement(2100..2528)
                     /locus_tag="ACHKG5_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0870700.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSHSLKGTLASPETEMQQIRREVMLGWPADALNQA
                     TNRVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(2542..>3222)
                     /locus_tag="ACHKG5_004770"
     CDS             complement(2542..>3222)
                     /locus_tag="ACHKG5_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870701.1"
                     /translation="DAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADWT
                     ATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSS
                     IGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVTAIKVTPTSGTVAKGKTTTLTVS
                     FEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVT
                     VTEAGAAG"
BASE COUNT          638 a         1030 c          748 g          806 t
ORIGIN      
        1 cccagctcag ccagctggcg tttgtactcc agcgtctcgt ctttatgcgc cagcagggat
       61 ttttcctgct cagataactg tcgtttcgtg gcagcctctt tcaggaccgc atactgactt
      121 tccgccttcc ataaatcacg gcgctgccgg ctgattttct cattcgcacc gctgtgtttt
      181 tccagcgtcc tgagctcagc ttcaagggca agcagagcct ctcttgcctg ctcctcttcc
      241 ctctccccgg cagagcgcgt tttcggtgat gtatgctttt tacctgtcag ctcttcagcc
      301 agacggctga cggcttcctg ctgccccgga cctttgctga cgcctgttgc acgcgagcgg
      361 ttgatgtacc ccatttcccc ctggcgtata cgcgcatccc gttccgcaat ggattttctc
      421 agcgccagtt catcgcgttt tgttttctca ataaatacgc ggttctcttc tgccagttca
      481 ccaaacaacg caccaacgcc gggcacattc tttgtcgttt cccaggctga ctgaataaat
      541 tcagccagcg ccagatcccc ctgcacaagc agcagcttca cttgttcaac ggttccggcc
      601 accacgtcag tgatcagact gagtgccccc agtgtatgat cacctatcca tgcccatgcg
      661 tcagaagtcc aggttttaac atcgtcccag attttttcca ccggcgtggc cgctttatca
      721 agttgctcca gacgtgcatt catgacatcc gcaaacaggg acatcgcctc cgtcaccgca
      781 acctgtttac ctttcgtgcg ctcaagctca tcaatatggc gtaactggga aacgctcagg
      841 aagttatact gctgattcag ggaggccagc gccttcaccg gatctgctgc aatcccttca
      901 aaggcttttt ccaccttccc ggcatcgtcc cccaccgtct gcagccatct ctgagaggtt
      961 tcccccatga tccgtagctg cccggcggta tatttcccgc tttctgccag acgggccaga
     1021 ttttctgccg cctgtctgat accaccaccg gcttcatcgc tgatcacccc ggccattttc
     1081 cacaattctg ccgttgtggt ggcagccgcc cctccggtca ggatcagtga acgcaataag
     1141 gcccggtcag cctgctctgc ctgccaggcg gcggcagcaa gcgcggccag tacggcaacc
     1201 ccgccacctg ccgccacacg ggccaccgac ataaatcgtc ccagctcacc ggcattccgg
     1261 gcattttcag ccagtgcatt tgccgtatct gacagcgact cctctgatga ttcagaggca
     1321 tccctgatcc cgagaagttc ctccttcagc agggtaagca ggctgagcgg tccaccgaat
     1381 gaatcgctga tctgcccccc ctgctgcagc atgataagga agggattctg accaccggca
     1441 agctgagtga caatatccgt gaactgtgcg ggcagtgtgc gcatggcagc cttatactgt
     1501 ccgactgata tcccggcttt ttgtgcagcc agcgcctgtc ggctcagact ctgctcagca
     1561 gcatcagcct gttttctgaa agactgactg actttcccgg acatcaaatc agcaagcgca
     1621 ctggtttcac ccagctcttt ttttacccgc gctgcctctt cagaaaaacg ggcagaatcc
     1681 agtgtcagca cggctgtcag atcggcaaaa ttacctgcca tcgtggtcac ctcctgaaat
     1741 gtcctctgat accatcaata actgtcacaa cctccttccc tcctccccga aacggactcc
     1801 accggcgagg cccgccgcct tctgcatcag tacatcattt tcgtccggcg tctccgcctg
     1861 cccttcctct gcctccggag cgaacaggct gaaatccgcc ggatgcatat ccggatcgca
     1921 aaaaaacagg ctgagtacgg cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc
     1981 ctgaaaataa tgcgtgcagt aaaaacgtcg ccagtcggca tattcggtgg atgtcatccc
     2041 ggcaagcatg gcgcgccagt cggctctccc catctcacgc gccagtttca ggacaaagtt
     2101 cagctcgcct tcgaatgctt ttttgatgtt accggctcag tcgcttctgt tttcccggtt
     2161 ggttcaggat cggcatcgtg ccggttatcc agcatacctg aaagataaag cacccggttc
     2221 gttgcctgat tcagtgcatc agcaggccat cccagcatca cttcacggcg gatctgctgc
     2281 atctctgtct ccggagaggc cagagtgcct ttgagggaat gggaatggga atgccatagc
     2341 gacatcgcca caagcaggga tgccgtttcc agatatcgct ggttaatgtg cacgacatca
     2401 tgcttcgttg tctcctgttg ttctgcgtct gaaacaaact ttatataatc aaaccgctgc
     2461 agcgcagaca attcggaaag cgtgacggac acaccgttat attcaaattg ttctgttttc
     2521 agaaacatgt atgacctccg tttaccctgc agcgcccgct tcagtaacgg tgacttcagc
     2581 cactgcggcg aactgaccat ttccgctcac cacagggatc tgcaccttac ctgtcgccac
     2641 gccgtttacc gtaattgtca tatctttcac actaatggtg gctttcgacg gatcggcgga
     2701 aaccgctctg aacgtcttgt cggttgcact ttccggctca aaagaaaccg tcagggtggt
     2761 tgttttccct tttgccaccg taccggatgt cggcgtcacc ttaatcgcag tgaccggcgt
     2821 aattttgctg cgttcttccg ctacagaagg tttgcccacg ttggtcactt tcaccgtgcg
     2881 ggtgatcact tctttcgccg tcacggcctt accgatactg ctgacccagc cacggaacac
     2941 atccaccgtg ccatttggga aacggatttt ataggcccgc acatccccgc tttcaaacca
     3001 gcctataagc cctttctgac cttcttctcc cggtttccag gccagcgtaa aactggtatc
     3061 tcctgcagac ttctgcccct gcccggtcgc ggtccagtcc gcgtcttcat catccaggta
     3121 gttatcatcg tagggttctg ccgtcatctc gcccggcgtc agatccttca ccttagccag
     3181 tcgctgccag tcatcgtctg acaacgggtt tgcataagca tc
//
LOCUS       DAZGXS010000253         3180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGXS010000253 DAZGXS010000000
VERSION     DAZGXS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..246)
                     /locus_tag="ACHKG5_004771"
     CDS             complement(<1..246)
                     /locus_tag="ACHKG5_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0870702.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATRDPEYYF"
     gene            486..629
                     /locus_tag="ACHKG5_004772"
                     /pseudo
     CDS             486..629
                     /locus_tag="ACHKG5_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(758..834)
                     /locus_tag="ACHKG5_004773"
     tRNA            complement(758..834)
                     /locus_tag="ACHKG5_004773"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Arg,seq:tct)
     gene            complement(848..924)
                     /locus_tag="ACHKG5_004774"
     tRNA            complement(848..924)
                     /locus_tag="ACHKG5_004774"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(888..890),aa:Arg,seq:tcg)
     gene            complement(934..1009)
                     /locus_tag="ACHKG5_004775"
     tRNA            complement(934..1009)
                     /locus_tag="ACHKG5_004775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Ile,seq:cat)
     gene            complement(1050..1175)
                     /locus_tag="ACHKG5_004776"
                     /pseudo
     CDS             complement(1050..1175)
                     /locus_tag="ACHKG5_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1454..1888)
                     /locus_tag="ACHKG5_004777"
     CDS             complement(1454..1888)
                     /locus_tag="ACHKG5_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0870703.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1974..2114)
                     /locus_tag="ACHKG5_004778"
     CDS             complement(1974..2114)
                     /locus_tag="ACHKG5_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0870704.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2111..2473)
                     /gene="rusA"
                     /locus_tag="ACHKG5_004779"
     CDS             complement(2111..2473)
                     /gene="rusA"
                     /locus_tag="ACHKG5_004779"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0870705.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2470..2760)
                     /locus_tag="ACHKG5_004780"
     CDS             complement(2470..2760)
                     /locus_tag="ACHKG5_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0870706.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2753..2923)
                     /locus_tag="ACHKG5_004781"
     CDS             complement(2753..2923)
                     /locus_tag="ACHKG5_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0870707.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2923..>3180)
                     /locus_tag="ACHKG5_004782"
     CDS             complement(2923..>3180)
                     /locus_tag="ACHKG5_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870708.1"
                     /translation="KTFATPDWVKDALKHTYLGYETKEPVDVVTGEITTIQSLRHTSD
                     LDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
BASE COUNT          811 a          852 c          716 g          801 t
ORIGIN      
        1 aaagtaatac tccgggtctc tggtggcgct gattactgca ccttctccac ctgtcagctt
       61 caccacaaca ggtgtgctca catcaccttc tgcgacaatc gcctgaataa gtgctgcgcc
      121 atcatccgta tacgaagaaa acggcccacc gtatggttgc catccttcac gaattttttg
      181 cgcaagtgca tcagcaaggt ctgacggcga cgccgccctg acaacatcat aatgtttaaa
      241 tgtcattatt cctcccggcc gggatagtgt attaaatcag atatggagtg ggctgtagtc
      301 cggaagcctg aatgacacac ggggactaca gcccaagaaa tgaaaaaagg ccacgcagtt
      361 gcgcagcctg ataaaccctg gttaaaatcc acacgataac aacacaacaa tatcagtatc
      421 tcatgctatt gcccgaaccc attcgggcat tttttaccca taaaaaagcc cctccggaga
      481 ggggcatgtt tgcatgcaca ttctttttct tgcatggtgc cgggtgcctc ccggtgaatt
      541 cagtatcagc acctgaatcc gcgattatca catatacctg gttgctgatt gcccctccgc
      601 acagggggat tcaccatgcg aaattttttt aacaaacgct cagcatgtca ggcaacattc
      661 aactgcctga attgtgaggc atttaacatt tcactgtccg gtgtctttcc tgtaataaaa
      721 agcccgcaaa agagagtcag ggcagataag tgtggtgtgg cgcgttgtac tggattcgga
      781 ccagtgaccg attgcttaga aggcaattgc tctgtccggc tgagctaaca acgctgaata
      841 ccgataatgg accgccatcg gggacccgaa ccccgcgcaa ccagcttcga aggctggcgc
      901 tctgtcctga tgagctaatg gcggtatgtg atatggtggc ccttgctgga tttgaaccag
      961 cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg ggcgcaggat
     1021 aataacggta cgtaactaat cctgcaatat catccgttct gactgactaa atcctgaact
     1081 tccctgaccg tctgctcaaa acgttcagtc tccagctcaa cgccaattgc acgacgcccc
     1141 agcgacattg ctgctttgac gctacggaca taaaaaagcc agccactggg ggaggctggc
     1201 aaactcgtag agcaaaatgc tgttacgcaa acttcgttac agggtcatcc tgcaatacaa
     1261 aaaatacaca atatttagaa aactaatagt gccatgtgca atttttaaga ttttgttatt
     1321 aattgtggtc gcaccttcct ttctgtgtac tttccgtata gctcacagga ttctgggtac
     1381 aaaaaaaccc gcgcatcggc gggttaataa gcagcgtggc aatgtaacca ctcttatcat
     1441 gatatgcaga tttttacgat cgtaaactat tttttcgctg ataaaataca gaggttctcc
     1501 ctcccggcaa ttcacgctca acataccgat ccatctcaag cctcactccc agcatcatca
     1561 gcatgccttc aacaatcccc tccgctttgt gaaggcgttt acctatacag gtgtcagagc
     1621 acccatgttt ccgtgccagc gccatgaacg tctcccccaa cacgtaataa tcaaccagca
     1681 agtcatgcag atcgcgattg ttccggtaaa ggcgggctat acacccgcat atcaccatcg
     1741 catcatcgtc acagcactgc gggcgtgatt ttactttttc ggggatcagt cccttaaaac
     1801 cggcggcaat ggacgaccag gtcacatctt catgattatt agccgcccac gctccccaac
     1861 gctcaagaac catctgaata tcacgcatca acttactcca caaaaatcag accagaacgc
     1921 caattacaag caaaaatcaa caaaacagta ttagttgatt gttatctctg acttcatact
     1981 cctgctcctg tcagggtttt ggcgtaattc ttcagtattc ggtaatcggt caaaacagaa
     2041 ccggggaaac gatataagcg cagacgcccc cagcggtggc gaagaagttc tgccatatta
     2101 aactcaaaca tcattcattc cccatttcgg tgatggtcag ttccagcctc ccacctttgg
     2161 taacaggcat cttcacaacg cggtaatcaa cgacctgagc atcatccagc cagaaacctg
     2221 ctttagtgag tgcgtcaaaa gcggcttttt gcagattatc caggtcacgg cgacggcgat
     2281 ccggcatgtg gcactcaatg cggattttca caggcatagc caggccgata tccagcattg
     2341 cgtttttaat gattcgggcg acgttatcgc ggtatgcctg cccctctgcg ctgatgtgcg
     2401 tgcgcccgcg attatggcgg taatagcgat tattgctcgg aggccagggt aatgtgatgc
     2461 tgtaggtatt cacgccttaa taaccccctc tttcagccag ataacctgtg ttctcgccat
     2521 accttccagc gcgcattctt ttgcatatcc agcgtcaaca aaatgcgtgc gacggtcgat
     2581 ctcatcgtga caggcagaac atgcaatggt ggcaatcagg tctggcggtt tggtaccggt
     2641 gccacacaat ccagccagcc ggatatgtgc cagtacagac gtttcagaat tgccattaca
     2701 tacgccaggg attcttacct ggcattcccg accacgcgct gcttttctca aatcagccat
     2761 gattcctcct tgctgccagt cgcaaccatt ttttatcaac caggctggcg gtatatccga
     2821 gcagagttgg tatttcggat ggcttcagct caggtttacg cttacgacga tttggtactt
     2881 tgtagatgtg tccgttcatg acacgaataa gcggtgtagc cattacgcct cctgcttgtc
     2941 gcgcagcagc tggaactcgc agctctgcgg aatagtcagg tggcagccaa tattcatcgc
     3001 ccaggcttca accttacaca ggaagacata catctctccg gtatcgagat cggaggtatg
     3061 acgtaatgac tgaatggtgg tgatttcacc ggttacgaca tcaaccggtt ctttggtttc
     3121 ataaccgaga taagtgtgtt tgagagcatc ttttacccaa tctggagtgg cgaacgtttt
//
LOCUS       DAZGXS010000254         3164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGXS010000254 DAZGXS010000000
VERSION     DAZGXS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..308)
                     /locus_tag="ACHKG5_004783"
     CDS             complement(<1..308)
                     /locus_tag="ACHKG5_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0870709.1"
                     /translation="MTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGA
                     KVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDIRLQLRDDALVL
                     "
     gene            complement(408..2516)
                     /locus_tag="ACHKG5_004784"
     CDS             complement(408..2516)
                     /locus_tag="ACHKG5_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0870710.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          721 a          895 c          769 g          779 t
ORIGIN      
        1 ttgagtacca gtgcatcatc gcgtagctgt aagcggatat cagaaggcgc tttccagccg
       61 gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt agctgctgta ggtgcgggag
      121 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
      181 cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc ccggacacac cgagcacgcc
      241 acgccggtgg gcgcgccaat tcttacacct gccgaaccct ggacaatagg accgccatac
      301 tgagtcatat ctccctaacg cgccgctggt tttccgctca tgattcaatt ccttcttctg
      361 tttattttta atgcttgttt gttactgaat accgaacagg tacgggatca gtactctctc
      421 atatccggcg tatcctgctt ttcatcaggg gtcttcggtg gggatttcga ggctgcacag
      481 ttccagttaa tcaacagtga gtcaccatta acctgctcct taccctgcaa atgaattttt
      541 gtgccgttga gaaaaatttg tccgtccttc gtcagcacca gccttgcctt accacagcag
      601 agctccagat gctcaccggc ggagtaaatc gcggtctgcc cggtatcatc ctttttcgtt
      661 ttaccaacgg tgaaggtaac gttatttccg actttgacgt cataacccag cccgaccctc
      721 aacgatttat gcaaaccgat ctgcgaggac tgcatcagag ccaccgaagt gttcataatg
      781 ccacctaccg tcgtctggta cgccacgccc acggtcagcg cacgaacaag tccaatcgtt
      841 tccacctgaa cagaacccac gttaatgatc tggttactgc ccaccgtctc tatctggtta
      901 acgcccaccg tctgtatctg attgttggta accgcaatca tctgattgtt gccaatggtc
      961 tcagcatggt tgtttatcac atccgtggtg cggttattca gcacctcggt gttcatgttc
     1021 ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat cgtcaaactt cagttcatta
     1081 aacccgctgc ccttatagtt tttcgaacga atggtcatct gcgtcttcgt ccccggcagg
     1141 ctgccgggtg tgcggttttc ctggtggtag gtacgcccca taataatcgg ctggtccgga
     1201 tcgccgttga ggaagtccac aatcacctcc tgacccacac gcggtatcgc caggttgcca
     1261 aagccggtgc ctgcccacgc ctgtgccaca cggatccagc atgaactgtc ctggtttgac
     1321 gggttatagc ggtcccagtt aaatttcacc cgcacgcgac catgttcgtc gcagaagatt
     1381 tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc catccaccag cggtttcagc
     1441 aacggctgtg gtcgccatgt ccggtctgcc ggtattaccg caaagtggtt attcagcgtg
     1501 gtacctgaac ccctgcgtcc cggcaccgcc tgtggctgct cgccgtgcag ttcacttgcc
     1561 accacctgcc attcccggtt caggttcgcc tgcggatgcc ccgtcagcac aattcgccgt
     1621 cccggccata tttccggaga acggcttgtt ccgcgcgcca cttctgcgtt gttgcgccag
     1681 ccatccatct gccagcgggc aaagttctgc ccgtgggcac ccttgaaacg tccggggtag
     1741 tcatacactt catactgtgt gcgctggtaa tcctggtact ggccttcctg atcaaaacgc
     1801 cctgcccagc cgggtcgttt aaaggtgtag tccttggtca ccacggaaga agggcggatt
     1861 tgtgcgctgt agcggaactg gctgatgcat agggtgctca cctcggtacg ggtgttcggg
     1921 ttccagggga tctcaaagga ctccggcaga taacgcacgg tgtcgcacag gaccaggctc
     1981 tggtcgatac ttttttgcgc gtgctcctca taaaagaaga tgccttcctc cgccgccatc
     2041 cggcacagga aatcgtaatc agtttcgccg tactggacac aaaactcacg ggaaggatgc
     2101 ggctcgctga acagcggact ccactcggtc acaccgtttt ctttcaggat cgtagcgagt
     2161 atgctttcga tgtcctcatt ctggaagata cggaagttct ggcgcagccc tgtgcgccac
     2221 agcggcgggc acaccttcat gctgtacagc atctggtttt tgtcgttctc ccccagttca
     2281 aaccaggtca ccacaccttt cacccggcgc tgtacgtcat cgccctgcca taccgtcagg
     2341 taggccattt tgtccagcac ctgcgcaaat tcaagggaaa gaaactgctg actgaccaga
     2401 gaaaggtcga gggaaaaaag tgaagagagt gactggttca gatgaaagga gaccaccgca
     2461 aacgcgtccg gtggcaggcc gtccacttcc agcgtgaaac gtaatccggt tgacatagca
     2521 acctccgtga aaatgaataa taatccgcac atattgcatg tacaacatgt cgggcggatg
     2581 aaataagtga gtcgacatct ggcgttaccg gggaatatca gaaaaatgtc atggttatgt
     2641 gatgatttag tctgggattt tacttcagag aaacgggtgt ctgtttagtc aacaaaataa
     2701 tcacaaacgc aacggatggg ttctcgacgt tattcacgac aggctcgtac attacagcga
     2761 gtaataaaaa acctgatatg caaagtcaaa ccaatattca taatgtcttt ttattcacaa
     2821 caataaataa tttaataaca atcattgaaa tgaatgatga tttgtataag gaatatacgc
     2881 taccgcccca ccctgataac atgatctgca cattgccatc ttaacgtcgt taaacactgg
     2941 cttaatagtg gatatttcaa attaggaatt tattaaattc acctgatgaa tatcataagc
     3001 aaatagcata gaaaataatg acaactcccc cgaggggacc atttccaaag cactgcacca
     3061 ccacctggtg cccggatgat cttgcttcag tggtaagagt acctgattca ttgttcccaa
     3121 aatggtgttt tcccctgccc ctccaataat gtgcagctct cacg
//
LOCUS       DAZGXS010000255         3163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGXS010000255 DAZGXS010000000
VERSION     DAZGXS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(6..632)
                     /gene="dsbA"
                     /locus_tag="ACHKG5_004785"
     CDS             complement(6..632)
                     /gene="dsbA"
                     /locus_tag="ACHKG5_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0870711.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(649..1635)
                     /gene="srkA"
                     /locus_tag="ACHKG5_004786"
     CDS             complement(649..1635)
                     /gene="srkA"
                     /locus_tag="ACHKG5_004786"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0870712.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1712..1981)
                     /locus_tag="ACHKG5_004787"
     CDS             complement(1712..1981)
                     /locus_tag="ACHKG5_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0870713.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2051..2635
                     /gene="mobA"
                     /locus_tag="ACHKG5_004788"
     CDS             2051..2635
                     /gene="mobA"
                     /locus_tag="ACHKG5_004788"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0870714.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2617..3144
                     /gene="mobB"
                     /locus_tag="ACHKG5_004789"
     CDS             2617..3144
                     /gene="mobB"
                     /locus_tag="ACHKG5_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0870715.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          827 a          768 c          763 g          805 t
ORIGIN      
        1 ttgaattatt ttttctcgga cagatatttc actgtatcag catactgctg aacaaaaaca
       61 tccatattgc tggtatccat accctgcgga ttcagctgat atttaccgtt aacaaacatc
      121 gccggaacac cacgcaattg cacgtcagct gcagcttttt cctgctgagc gaccagagat
      181 ttcaccacga agctgttcca cgccgcgtcg tactcttcac ctttaatacc tgcgttgata
      241 aatacatcgc ggatatcaga tgctgaacga atggtctggg ttttctgtac gccttcaaac
      301 agcggaacag tcactttgtc ttccacgccc agcgccatcg ccacagccca tgcctgagtc
      361 agatctttgc ccaggtcacc acccatgaag ttgacgtggt atttagtcat cttcacgcct
      421 tccggcagtt ttttcttcac attatcagaa atatgcagaa cttcttcaaa ctgatagcag
      481 tgcgggcaga agaaagagaa aaactccagc acttgcggcg cgccagctac cggtttttcc
      541 agggtagtgt actgtttacc atcttcatac tgcgccgccg atgcgctaaa cgctaaaact
      601 aaaccagcca gcgccagcca aatctttttc atgatctact ctctccgatt aatacatagg
      661 tgttaattgc aaagggggtt cttgtagaac ttttgcctgt tctataaaag tcgccgtctg
      721 tcgtagccag taatcttccc cggttaacca cgggaaattt ttcgggaacg cgggatcagc
      781 ccaacgccgc attagccagg caagataata aaccaaacgc atggcgcgta aaggttcaat
      841 cagtccgatt tcagcggtgt cgaactcgct aaattcttca taagcttcaa taatagtttc
      901 cagttgcatc cgctgctcgg ctttatcgcc attgagcaac atccacaaat cctgaatagc
      961 tggaccatta cgtgcatcat ccagatcaac aaacattgga ccatcgcgcc agagaatatt
     1021 cccggcgtgg cagtctccat gtagccgcag aacggtgaaa tcttcccgcc agtgtgctgt
     1081 aacggcggcg atcagctcat ctgtcgcttt caggaatgcc gctttcaacc cggaaggtat
     1141 cagtgtagcg tcctcaaaca gcttgcgtgg ctcaatgaga tattcattca gaccgatggt
     1201 cgggcgatgg ataaaaagct gtttgcgccc cgtctggtgc atacgcccta aataacgccc
     1261 aaccgcttcc atctgatcga tattatcagc ttcgaactgg cgaccaccga cgcttggaaa
     1321 aacagcgaaa taaaatccct gatgattcaa taaagtctga ccgttaaaag ccacaggtgc
     1381 tgcaaccgga acttcatcat ttaccagctg caacgcaaat tgatgttctt cgaggatttg
     1441 atcggctgtc caacgttcag gacgataaaa tttgacgaca aaacgtcgac gatcttcgtc
     1501 ctgaaattga tagacacggt tttcatagct gttaagcggg gtaagaccgg aatccacccg
     1561 gatcccttgc tcaaacagag cgtccatgat ggtatccggg tgtagtgtct ggaaagtaaa
     1621 agcgctgttg ttcatcccat catccggaaa acattacgaa tgatgaagga tatcatttca
     1681 cgatgatttt ggtggcgccg cttacaagct tttactcttt aattacgccg cgagcgcgta
     1741 acagcgccgt tttgaagtct tcttcataat ctttctgcaa acccgggatc accgcatctt
     1801 tcgcggaatc acgcattttc aggtgataga tcagaatgtc atccgtcaaa tccgccagtt
     1861 cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa ttgcaacagg ttaaggtctg
     1921 gctctttttg ccaggctggc tggaggagtt caataacttc attcagacgt ttacatttca
     1981 tagtaatgct ccttactctt gagacagaca cgttagcagg gtcaatccca caataaaaga
     2041 ggcgatatcg gtgaatctga tgacgacgat aacaggcgtt gtgctggcag gcggtaaagc
     2101 cagacgaatg ggcggcgtag ataaaggatt gctggactta aatggcaaac cgctgtggca
     2161 acatgtcgct gacgcgctta tgacgcagct ctctcacgtc gtggttaatg ctaatcgtca
     2221 tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa gattcactgg cggattaccc
     2281 aggccctctg gcagggatgc tttcagtaat gcagcaggaa gctggtgagt ggtttttgtt
     2341 ttgtccgtgc gatacgcctt acattcccca tgatttagcc gcccggctta atcatcagcg
     2401 caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc gatcacccga ctattgctat
     2461 ggtaaaccgc gctattgagc ctttattact agaatatctg caagcaggag aacgccgggt
     2521 aatggcattt atgcgtctgg ctggcggtca tgcggttgat ttcagcgatc ataaagatgc
     2581 atttgttaac gtgaatacgc cagaggagct tgcccgatgg caggaaaaac gatgataccg
     2641 ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta cgcttttgaa aaaactgatc
     2701 ccggcattat gcgccagagg gatccgtcca gggctgatta agcatacgca ccatgatatg
     2761 gatgttgata agccaggcaa agatagctat gagctgcgca aggctggcgc ggcacaaacc
     2821 atcgttgcca gccagcaacg atgggccttg atgacggaaa caccagacga agaagagctg
     2881 gatctacagt ttctcgcaag tcgaatggat acctcaaagc tggatttgat tctggtcgaa
     2941 gggtttaagc atgaagagat cgcaaagatt gtgctgtttc gcgatggagc cggacatcga
     3001 cctgaagaat tagtgataga caggcatgtt attgctgtag ccagtgatgt gccgcttaat
     3061 cttgatgtcg cattactgga tattaatgat gttgaggggc tggctgattt tgtggtggag
     3121 tggatgcaaa atcaggacgg ataagcaaga gtaggctgga gca
//
LOCUS       DAZGXS010000256         3156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGXS010000256 DAZGXS010000000
VERSION     DAZGXS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(262..1740)
                     /locus_tag="ACHKG5_004790"
     CDS             complement(262..1740)
                     /locus_tag="ACHKG5_004790"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0870716.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1767..3044)
                     /locus_tag="ACHKG5_004791"
     CDS             complement(1767..3044)
                     /locus_tag="ACHKG5_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0870717.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          844 a          810 c          712 g          790 t
ORIGIN      
        1 ccagttcata ctttaagtga aaattgataa agtgcgattc gtattgtctt ttattctaaa
       61 gacatcgagt gtagttaata ttccttgtaa aaacagggat aaaccgaact agttaaagtt
      121 tttattatgt tccctgcatc agcggggata aaccggactt gttatcattt tttaagttgt
      181 cgaacttatg ttccccgcgc cagcggggat aaccattcag gtatgttcag gccgggcagt
      241 ttcccgcccg gtcttcctca cttacgcatt cgaccagctc tggatcgaat ccgcattccc
      301 caattcccca tgcatcactt catacgattt tttcaggatc acatcagtat gtttggttag
      361 ggctttgaaa atacctttat tcatcgcgtc gtagcgtttg gcattgctct caatcggtat
      421 aaagatatct ttcacgcgaa ccattttgtc gacagccgtt gcgtaatccg ggtatagccc
      481 cagacctacc gccgtattga tcgctgcccc cagacttgca caaccgttaa tggcgttacg
      541 tcgtgccgga aggttgaaca cgtcggcaaa aatctgcata aacagatcgc tgttcgaacc
      601 gccaccagta atgatcacat gcttcgcaaa gtgattcatt tcattacaca tattgtcgta
      661 attgttcttc agcgtcagcg ccacgctttc cagtatcgaa cgatatatcc atgcgtaatc
      721 catgctggaa tcaaagccga tcataatccc gcgtttgtac ggttcccacg gattggtcag
      781 ccagtccagc accgtcatca gcccattaca gccaggtggc acacaagaag cttttttgtt
      841 gagtaaatct tccggtgaaa gatcctgcgc cctggcatcc tgaattaacg actcgcctaa
      901 catgtcgcgc agccagctca ccgtccacat gcctttgcga ataccgtaac cttcatacag
      961 caatgtttgc ggaatagaag acataatcgg ccagtacgcc accggatctt tcggcaaggc
     1021 tttgccgttc atcatcagcg cgatataagt accgagtgaa atcaccgccg tttcatcatc
     1081 cagtaatccg gctcccagag cttctaccgg tttatcactg gtggtacaaa caaccggcag
     1141 ccctgccggg aaatgtgtcg ccagcgcggc ttgtggtgtg atctgtccaa gaacagtgcc
     1201 tggcatttgc acatcaaaca gcatatgacg ggggatatta aacttctcca ttaccgcagc
     1261 atcttcgctc catgcccagc tcttataatc caccggccac tgaccaaaat agttggcgat
     1321 attgtcttta aactcgccgg ttaagcgatg cgtcagataa cccgaaaaag aggtgacata
     1381 ggccacgtca ggattagtgt gttcgtaggg acgcgttacg cgtgcatcct gccagctaat
     1441 caacggcgca gcaggcgtgc catcggcttt caataacgca cggcagcaac ggatggatcc
     1501 cagaccaata ccgacaatat cttctttatt cccggcaaac tgactcatca aatcgtgacc
     1561 ggcaaaacat aatgatgccc ataaatcgtc gtcaggatgt tctgcggtat cggcatctgg
     1621 cgtgtgcatc ggctgcaata aacctttgcc ttcgcaaacc acgttacctt ccagatcgta
     1681 catcaccact tttgtgctct gacttccgcc atcaatccct atgatgtatt tcttcgacat
     1741 gatttttctc ctgtaatttt cctggcttac ttgctgctgg ccattgcggg ggcgctatca
     1801 gcagtacgaa ttttttggaa cagcaaaaag gtaaagacaa tcaccatggc aagcgccgcc
     1861 atgcccatca gccacatatt gcggtaggct tctgctgcgg gctgggtgtc ttgccagtgg
     1921 ccgataattg ggtaaacgaa gacatccggc aggaatccaa tcaccgagca aatacctacc
     1981 gtggtgccca taatgtaaga cggtgttctc gcttcgccag ggcaggccca gtacagcccg
     2041 cgtgaggcgt aacaggtgaa tcccagcagt aaaatcaggc caatccccat cgcgaccgat
     2101 tgcgggttag agttcgtgac gagcagggca gttaacgcca gcaggccgag tacggaaagg
     2161 atttggatca cgcgggtagg ggatttcact ttactgtagg tggtgataat gccgccaagt
     2221 gggccgcaca gcgcgcggaa gattttgttg atcacaatcc ccatgtagct cgccgccacc
     2281 agcgacatgc catacatttc ggtcagatag ttggtggagt aactcagaat ggcgtagatg
     2341 gtgaagacgc caaaaatcac catgctgcaa taccaggtgg tgctgatacg caaaacggca
     2401 aggatgtcgc taagctggaa tgactgtttt tcttcgttat tggcactgcg caggttgttg
     2461 ttatcgctaa caaaaaacca gcacagaatc cccaacaaga tgtaaaccac actatagatg
     2521 ataatgaccg ttttcaggct ggtgctgtca tccggtgcaa agcgagaaaa aacccacatg
     2581 gtaaacactg ccagcgacat tacaccgacg ccgcgcagcc cttccatcca gcccataatt
     2641 ttcccttgct cgctatgatc gccaagcaac gatgcggctt taatcgacac cgaccacagc
     2701 attaaaatcg ttgttatcgc aaaggcgacc tgaatacaga gcattaccca cagcggtgga
     2761 tacgttgcca ttaacagacc cagtaatccg gtaatgatca tcgcggaagt aatcattttg
     2821 cggtgtgaaa atttatcggc aataacgccg ctgggggcat aaagaataat agccgcaata
     2881 ccaaaggtac tcattattaa acctatctcg gtattgctga atcccataaa tttcgccatg
     2941 ggaatttgat aaatataacg taaataagcc aggtcgaaac taacgccgcc gctaaaacta
     3001 attatcgcga gggttatcca acggcgatat gagttgtgtt gcatatcgta ttcccatttg
     3061 taatagacaa aaaaaagaga aaagtaagcc gccctttcag gtggattact tttctcaggt
     3121 ctctagtaat tgaaataata aataacatgg aaagca
//
LOCUS       DAZGXS010000257         3151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGXS010000257 DAZGXS010000000
VERSION     DAZGXS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..209
                     /locus_tag="ACHKG5_004792"
     CDS             <1..209
                     /locus_tag="ACHKG5_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0870718.1"
                     /translation="RGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASPK
                     CPQGPTPIELLKQEYERRKAAGFV"
     gene            243..959
                     /locus_tag="ACHKG5_004793"
     CDS             243..959
                     /locus_tag="ACHKG5_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0870719.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            956..1273
                     /locus_tag="ACHKG5_004794"
     CDS             956..1273
                     /locus_tag="ACHKG5_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870720.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1270..1572
                     /locus_tag="ACHKG5_004795"
     CDS             1270..1572
                     /locus_tag="ACHKG5_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0870721.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1562..1879
                     /locus_tag="ACHKG5_004796"
     CDS             1562..1879
                     /locus_tag="ACHKG5_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP0870722.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            1833..2150
                     /locus_tag="ACHKG5_004797"
     CDS             1833..2150
                     /locus_tag="ACHKG5_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870723.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2137..2574
                     /locus_tag="ACHKG5_004798"
     CDS             2137..2574
                     /locus_tag="ACHKG5_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0870724.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2576..2767
                     /locus_tag="ACHKG5_004799"
     CDS             2576..2767
                     /locus_tag="ACHKG5_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870725.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2770..>3151
                     /locus_tag="ACHKG5_004800"
     CDS             2770..>3151
                     /locus_tag="ACHKG5_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870726.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          886 a          655 c          929 g          681 t
ORIGIN      
        1 acagagggat tgagcggcag atgacggaag gtgagttaaa acgacttgca gaacggcaac
       61 tgacgaaatg ggcaaagcaa gttggtaacg ggatgagtat tcccccgatc cggagacaac
      121 tcgcatcgcc gaagtgcccg caagggccaa cgccaattga attactgaaa caggaatacg
      181 agcgccggaa ggcggctggt tttgtttgaa tctgagaaac gattttgtcg gaggaaattt
      241 taatggaaac cgtatctgac gcactgaaag cactgaaaag agcctcttca catgtggtgg
      301 cagctcgcct tggaattagt cgtgaagagg ctgtcaacga actgtggaaa ctgaaacgcc
      361 gtggagaagc ggataacaag ggggcgatat ggtggctgac tcaggctggt gaaagtgaac
      421 cggtgtcacc ggtaccgaaa gtgacggcgc aaatgctgac agaggcgatt gaacaacatg
      481 gcccacaaac ggcggatgaa ctggcattga tgttcggaat tacctcccgc cgggcgaatt
      541 catcgctggc aatggcaatc agcaaagggt gtctgattcg cgtgaatcag gatggtaaat
      601 ttcgttactg cataccgggc gttgatttac cggcagagcc gaaagcagca tccgtagctg
      661 aaacggaggg taaagccctt cctcagccag ctggtgttgc gttaccagtc caggaaacgg
      721 ctgcacagga agaaattaaa acagaagcgg tggaggacat tgtgaagttg cagcaatcgt
      781 tcactgaagc gaaagcagat gacctgattc taccatcgct gcatgtggct aaccgcgagc
      841 tgcgccgggc gaaaagtaat gttcagaagt gggagcgagt ctgtgctgcg ctacgggaac
      901 tgaacaaaca cagggatatt ctccgggata ttaccgccac cagagagcag cagcggtgag
      961 tggctggaag aagtggcgct gggctgaaat cctgatactc cggcagtgtg cgggaacgat
     1021 gagagtcgaa agcatcggtt atctgattgg ccgtagtgag tcagccgtca ggacgaaagc
     1081 gcgggaactg ggtatcagca tgatgttacg gggtgattat caccagtcag ccaaatgttc
     1141 acagcgtgat attgagctgg cgtggcaact gcatcagcgt ggtgtacccc gacgggaaat
     1201 tgccgaaaag tttgggatga agttgggcgc agtgaataac tacgtttatt tcgacaggag
     1261 ggttcaggag tgagggtgag ggtttatatc gccggtccga tgacgggata tgaaaatttt
     1321 aaccgcgagg catttcacaa aacggaagag gtgctgaaac gggaagggca taccgtttta
     1381 aacccggcag tacttccgga cgggctgact cagccacact acatggatat ttgcatggca
     1441 atgctccgtt gcgtggatgc ggtttacatg ctgaaaggct ggcagcagtc ggcaggtgca
     1501 ggggctgagc tggcactgga ggagaaaccg ggccatgcgg tgatttttca ggaggtgggc
     1561 agtgaatatt gacccggcga taacgattga tatggccctg aacgccggcc tggcacttct
     1621 tggttatttc tacattatgt tctgcagcgg acgatggctg tcactgttgt tcatgaaaaa
     1681 atggaataaa cgccgtaagc aggagcaacg ccagaaggca atggatgcat ttttcgaagc
     1741 cttcggaatt gacggcatgg aaccagggga tccagctcgc gcaatcagca gagggggtgt
     1801 agtaatcctt gtatatcgga gtgaagagaa aaatgacgat cacaaaacaa cgtgtagaag
     1861 aaatcatatc ccgcattgaa atgtatggac atggtgcagg gtatatcgca gacgaggtta
     1921 atgatctggc tatactggcg ctgaatttat caaatatcgc gaacctgaag cgatacgagc
     1981 ttgatatggg aggttgcgac tcgtgcggtc aggattgtgg cgctgatatg actgaagatc
     2041 ctgatggtga ttatgtcctg tttgatgacg tggttaagtt gtttgaattt gatacaacca
     2101 ctcaaaagtt agaaatcccg gcaaaggagg cagccagtgg gcaagattga ctatcaggca
     2161 ctgcgtgagg cagcacaaaa ctatcaatcg acgctggcgt ggtatcaggc taccccggac
     2221 agcccaaatg ctgaacggga ttgtgatgcg gctcttgctg cgtttaagcg tcacatccgt
     2281 catcgggaag cggatattat cgctgatttg ctggatggac tggaagaagc aaaatcacaa
     2341 ctcaacgagc agcgtgagta ttacgaaggc gttatctctg atgggagcaa gcgtattgct
     2401 gaactggaag cgcgggaagt tcaattaccg actcgctacg accttcgata tggacacccg
     2461 ataaatgcag atgagcgaca tgtcatgata cctaaagaaa atggcagttg gctttacctg
     2521 attgacctag aacacgcatt acgcgtcgct ggcattcgca tcaaaggaga gtgagatgaa
     2581 cggacaaata tcaattgttc gaccaggagc atgtgacgat cgcgaaatac gaatgattat
     2641 tcgtctggcg atggggaaaa caataactgc tctcattact ccagaaaatc tcgcattagc
     2701 attaacagga aagtcagaca tgccagtaga gctaaagctg cgaaatgttg agattaaggt
     2761 gaaatagcta tgaccactat taccaaagat cgactgctga caatccagca ttggcgcgaa
     2821 acatacggac cgggtagcaa cgttgtgctt ccagcagaag aagcggaaga gctggcacgg
     2881 attgcgctgg catcactggc agcagtatcg gatgaacgag cagcctatga attatttatg
     2941 gagaagcgtt tcggagaatc tgtagatcgc cgcagagcaa aaaatggcga tagagattac
     3001 atggtatggg atatggcgct tggctggatt atctggtgtc accgcgccgc catgcttcag
     3061 gctggaaact ttcgggaaaa taagggttcg tcaaccaata attttcggga aatctcggaa
     3121 acgtcaacca actatccggt aactccggat g
//
LOCUS       DAZGXS010000258         3082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGXS010000258 DAZGXS010000000
VERSION     DAZGXS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..184
                     /locus_tag="ACHKG5_004801"
     CDS             <1..184
                     /locus_tag="ACHKG5_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0870727.1"
                     /translation="YGWFPGTDGALITGLTFLDPKDATQVQGMFQHLQVRFGDGPWQD
                     VKGLDEVGSDTGRTGE"
     gene            186..455
                     /locus_tag="ACHKG5_004802"
     CDS             186..455
                     /locus_tag="ACHKG5_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0870728.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            457..564
                     /locus_tag="ACHKG5_004803"
                     /pseudo
     CDS             457..564
                     /locus_tag="ACHKG5_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            632..1612
                     /gene="nleB2"
                     /locus_tag="ACHKG5_004804"
     CDS             632..1612
                     /gene="nleB2"
                     /locus_tag="ACHKG5_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFP0870729.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVD
                     AHPYYDGLGKGVKKYFNFTPLHNYNHFCDFIEFNHPNIIMNTSQYTCSSW"
     gene            1646..2665
                     /gene="nleC"
                     /locus_tag="ACHKG5_004805"
     CDS             1646..2665
                     /gene="nleC"
                     /locus_tag="ACHKG5_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP0870730.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
     gene            complement(2785..2889)
                     /locus_tag="ACHKG5_004806"
                     /pseudo
     CDS             complement(2785..2889)
                     /locus_tag="ACHKG5_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          977 a          529 c          709 g          867 t
ORIGIN      
        1 ctatggctgg ttcccgggta cagatggtgc gctcatcacc ggactgacct ttcttgaccc
       61 caaagatgcc acacaggttc aggggatgtt tcagcatttg caggtcagat ttggtgacgg
      121 gccatggcag gatgttaagg gactggatga agtgggcagt gatacaggca gaactggaga
      181 atgacatgaa tattttgaga aagcttatgc agagtctgtg cggttgcgga aagcatgatg
      241 actgtgaaaa cggacggtca cttacagcac aattgcgact gggaccggca gacattctgg
      301 agtcagatga gaatggcatt attccggagc aggacagggt aatcacacag gtggtgatac
      361 tggatgcaga taaaaagcag atacagtgtg tggtaagacc gctgcaaatc ctgcgtgctg
      421 acgggacgtg ggaaaatatt ggcgggatga agtaacccga cagcttcaca aaaccggagt
      481 ccggctccgg tttttgttgt catgtccggt ggatgtttgt taggaaagca aagatggcaa
      541 aactgctgga ggttttgtgg ttgagtatgc caatataatt aatagattaa agagttagtt
      601 gtgaagaaaa tatggataaa caggacgacg aatgctttca ccgataagga caactttcca
      661 taactcagta aatatagtgc agagttcacc ctgtcaaacg gtttcttttg caggaaagga
      721 atatgagtta aaggtcattg atgaaaaaac gcctattctt tttcagtggt ttgaacctaa
      781 tcctgaacga tataagaaag atgaggttcc aatagttaat actaagcagc atccctattt
      841 agataatgtc acaaatgcgg caaggataga gagtgatcgt atgataggta tttttgttga
      901 tggcgatttt tcagtcaacc aaaagactgc tttttcaaaa ttggaacgag attttgaaaa
      961 tgtaatgata atctatcggg aagatgttga cttcagtatg tatgacagaa aactatcaga
     1021 tatttatcat gatattatat gtgaacaaag gttacgaact gaagacaaaa gagatgaata
     1081 cttgttgaat ctgttagaga aagagctgag ggaaatttca aaggcgcagg attctttgat
     1141 ttctatgtat gcaaagaaaa gaaatcatgc atggtttgat ttcttcagaa atttagcctt
     1201 attaaaagca ggagagatat tcaggtgcac atataataca aagaatcacg gtatttcatt
     1261 cggggagggg tgtatctatc ttgatatgga tatgatactt acaggtaagc ttggtacaat
     1321 atatgctcct gatggaattt caatgcatgt ggatcgtcgt aatgatagtg taaatattga
     1381 aaatagtgca ataattgtta accgtagtaa tcatcctgct ctacttgagg gactttcttt
     1441 tatgcatagt aaagtagatg ctcatccata ttatgatggt ttggggaaag gagttaagaa
     1501 atattttaat tttacaccat tacataatta taatcatttt tgtgacttta ttgagtttaa
     1561 ccaccctaat ataatcatga acacaagtca gtatacatgc agttcatggt aaatgaattt
     1621 gatatagttt attttgttgt aataaatgat ttgcagggta ttagatataa acatgaaaat
     1681 tccctcatta cagtccaact tcaacttttc cgccccggca ggatactctg ctcccattgc
     1741 tcctaatcgt gctgaaaatg cctatgcgga ttacgttttg gatataggta agcgaatacc
     1801 actttccgca gcagatttaa gcaacgtata cgaaagtgta atacgcgccg tccatgacag
     1861 ccgtagcagg cttatcgatc agcatacagt cgatatgatc ggcaacactg tacttgatgc
     1921 tttgagccga tcacagacat ttcgtgatgc cgtaagctat ggcattcata atgagaaggt
     1981 acacattggt tgcattaaat acagaaacga atacgagctt aacgaagaat cttctgtcaa
     2041 aattgatgat attcaatcac taacctgtaa cgaattatat gaatatgatg tcgggcaaga
     2101 gccaattttc cccatttgcg aagcaggaga aaacgataac gaagagcctt atgtcagttt
     2161 tagtgttgcg ccagatactg actcttatga gatgccatcg tggcaggaag gactgattca
     2221 cgagattatt catcatgtta ctggatctag cgatccatct ggagatagta atatagagtt
     2281 aggacccacc gagattctcg cacgtcgtgt cgctcaagaa ctgggatgga gtgttcccga
     2341 cttcaaagga tatgcagagc cagaacgtga agctcatctt aggctacgta acctgaatgc
     2401 ccttcgacag gctgccatga ggcatgaaga gaatgagagg gctttcttcg aaaggctggg
     2461 tacgatcagt gaccgatatg aggcgagtcc tgatttcaca gagtattccg ctgtgtctaa
     2521 cataggatac ggatttatcc agcaacatga ttttcctgga ttggctatca acgataattt
     2581 acaggatgca aatcagatcc aactgtatca tggcgcccct tatattttta catttgggga
     2641 tgtggacaaa cacaatcagc aatgattcgt ctttgcagtg acataaggtt actattcata
     2701 cattttaacg gagttgatga tgggtaatcg tgcaacattg tatgtaaagg caattcccct
     2761 aattttactg tagtaagtga acatggccgg aacgagacta tcagcatcat cggcttcggc
     2821 ccagtaaaaa gaggctcgga aaaatgcaca ataggcatca cacgtcatgc atggattcaa
     2881 attgtacata attcaacagt acagctataa atcgtaaaga aactgcagta cgttgtgcac
     2941 agaaaagtac cgtgattcac tattgtacag gtccattgca gcaacaatat tttgtgaatt
     3001 ttgcgtgaga gaaagaaaag gaataatttg tatataaaca attgattaat caattgctgg
     3061 aaaataaata aaactgataa tt
//
LOCUS       DAZGXS010000259         3053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGXS010000259 DAZGXS010000000
VERSION     DAZGXS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(432..1409)
                     /locus_tag="ACHKG5_004807"
                     /pseudo
     CDS             complement(432..1409)
                     /locus_tag="ACHKG5_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1787..2461)
                     /gene="nleE"
                     /locus_tag="ACHKG5_004808"
     CDS             complement(1787..2461)
                     /gene="nleE"
                     /locus_tag="ACHKG5_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP0870731.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
     gene            complement(2510..>3053)
                     /gene="nleB"
                     /locus_tag="ACHKG5_004809"
     CDS             complement(2510..>3053)
                     /gene="nleB"
                     /locus_tag="ACHKG5_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP0870732.1"
                     /translation="RDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAM
                     LKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKS
                     LENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDF
                     IEFKHENIIPNTSMYTCSSW"
BASE COUNT          835 a          673 c          579 g          966 t
ORIGIN      
        1 aaaaaaaata tttatctcga tgctacccaa catacgactc atttattttt ctcggtttct
       61 ccttgtatat atatgttgct ttataatttt actacgcacg tcaggcgtat ctgtaacata
      121 gctccgcatt atgcaatgcg actttgcata caaataggaa taaatctaat agctgtagat
      181 ttgctcctgt atttcctgaa ccaagcgagc cagtacatga tgcatatgtc tggaaaataa
      241 ttcagagtaa ggataagtcc agctttgttg aataaatcag gtttggaata agccccctca
      301 gttggtagct actgtcaggt aatacgcacg tattatgaca ttcgtgccct cgtcattttg
      361 tttagtacac gcactgtagt ggcacagaac ttctgcgacc tgaccatcct aaatagctgc
      421 gccgaatacc gctacacttt taacagccca tatttaccac cggggaatgg gctcacggtt
      481 tccataaaat aatgaacatt tttaagcagt taccacattg aacggcactg atgattgcgc
      541 gttatcgtat cgtgaagcgc ctgctataac cgctcaacat gattcaccca cggcgagtaa
      601 actggctgat aaatcactct gaacttcgga ttttctttca gccagcgctg tgtttcccga
      661 cttttatgga tgatgtagtt gtccactatg agcgtgatag gtttatcccg tcggtatgtc
      721 aatttcagat gtttcaacag actgataaat aaggctgagc ttttgctgtt gccacccaca
      781 tagctgactt tgcccgttcc actatgtaaa gcaccagcca gatagtattt ttcattctgc
      841 cccggtgtta ccacatgttt ttgctgtctg cgaagttgcc agtccgcacc ggttttggga
      901 ttaagataga tatacacttc atcttcataa aagaccggat gctccgcact acattcgtcc
      961 agtgctttgt gaattgctgc tttatgcggg ttacgggtac gcagggttgg cgcagccctg
     1021 cgccacacaa gcccgactaa tggcaaccag cggcgaacgg ttccggcatg taactggcac
     1081 tcggttatct cgttgatttt tattgccagc agttctgtac tccagcgtga acgctgatag
     1141 ccaaaatatc cgggagagtg ttttatcagt tcacgaaaca atgtgcagat atgctcaaac
     1201 ggccagcggc gggaacgtcc tgcgggtaat gatttcagac cttcaacact cgacagcgtg
     1261 aaccagttaa tccagcgcct gacggaggaa cgggcgcagc agagcgttct ggcaacgtca
     1321 ctgacgcgag cgctccggtg cagcatcagc atggcggtca gcctgcgggc ataattttta
     1381 tcgtgagttt tatagattgc tttttgcatc aggcgtcatt cgtcgcgggg aattggtgct
     1441 atgatcggca tcgctcagtc cggttagggg gggatttttg gcgattgatc agatcgcaca
     1501 atccgggctg agttcccttt cattgatcta ctattcggcg cagctattta gttggtgatt
     1561 ttgaacttct gctttgctac taagcagcct gcgttttcag gaaaatacgt gatgtgatcc
     1621 tgcaactcag caacagttcg gttgattcaa caaagcgttt tttaacaata attcttcatt
     1681 gtaaaaagca tcttcagttg tgatcaaata ttattattct tattggtgtt ctcatataat
     1741 gaaatagaat taattttagc ccccctacac aagtggctga gcttctctac tcaattttag
     1801 aaagtttatt atttatgtat ttcatataac tgtctatttc cccaggcatg tagctaaaga
     1861 tttctcccat ttcacgtgcg ataatcattt cagattttac tgcctttaac tctggtaact
     1921 cataatttaa ttcctgcatg atatctcttt ttatattata taattctata aatctatttg
     1981 cccgttgttc tcctagaggg ctgtcatttc tgtacacaac aactatgtat aaaaaatcat
     2041 tattttcttt agggaagatc ttacaacaaa tgtccggaag ctcacataat gcatgtaaat
     2101 caggattgta taaactatct acagaaaact gccccattgg gcgttttccg gatataactg
     2161 ttagccagct tccttccggt gtactggctt catttctgga aataggttca aacgcttctg
     2221 gacatacaga atacatagtc ttcatataag aaaaccgtct ggctttctcg ttaacaaatt
     2281 cttcctgttt tagttcagct actcctctgg ttataccgga tatatacttc ttatcatata
     2341 tattgggtga ttcattaaag taatcatgtt taatttccgg gtaaatattt ttcaccacat
     2401 gttcagcata tttagtattt ataggggaca cgccctgagt attagtaaca ggattaatca
     2461 tctgaatcta tacctaaaga acgatatttt tcaaccacaa ccggaagact taccatgaac
     2521 tgcaggtata catactggta ttcggtataa tattttcatg cttaaattca ataaaatcac
     2581 aaaaagcatt ataatcgtgt aacgatgaat agttaaaatg ccgcttgata ccctttccta
     2641 gaccatcata atatggatga gcgtcaactt tactcttcat aatatcgagg cctgcaagta
     2701 atgctggatg attactacga ttgacaacta tcgcaccatt ttcaagactt tttatctcat
     2761 cattacaatc tacatgcaca gcgataccat caggagcata caggactcct aatttatcgg
     2821 taataatcat gtcggcatca agatatatac atccgctaca gggcgatatg ttatggcatc
     2881 cagtttttcc cgtctctgta aacaaacttc cagccttcaa catggccaaa ttcctgaaaa
     2941 aatcaaacca agtatgttct cttttttctg catatgactc aatcaaagag tccttacctt
     3001 ctgggatatt ttttaactct tctcttaata agcctaacag ataattatcc ctc
//
LOCUS       DAZGXS010000260         3038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGXS010000260 DAZGXS010000000
VERSION     DAZGXS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..2321
                     /locus_tag="ACHKG5_004810"
     CDS             <1..2321
                     /locus_tag="ACHKG5_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0870733.1"
                     /translation="AENARLVSLLEAHGIEWRRKPQTPVQRVSVLSTDEKVALFRRLY
                     RGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDCAHRQLIPVSDLVIY
                     HHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCDELGVPAALEISRSR
                     QGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRLFPNQDTMPKGGFGN
                     LIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDIEPTILRATGSIHPL
                     DVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEKAQLPQVLINRLIRL
                     AAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDSVLSFLRDNNIAAEL
                     IDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFGKTVTAAAVIAKRKV
                     NTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNIDIAVVQSISRQGEVE
                     PLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRRDGLHPIIFMYCGAI
                     RHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDHDRTVAIAEEAMKAF
                     GQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAMLISGLNALPPDSPR
                     ILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREHTGKSDVRIIDFVDT
                     AYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(2483..2659)
                     /locus_tag="ACHKG5_004811"
     CDS             complement(2483..2659)
                     /locus_tag="ACHKG5_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870734.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(2797..3038)
                     /locus_tag="ACHKG5_004812"
                     /pseudo
     CDS             complement(2797..3038)
                     /locus_tag="ACHKG5_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          756 a          650 c          786 g          846 t
ORIGIN      
        1 gggcagaaaa tgcccgtctg gtctcattac ttgaagctca tgggattgaa tggcgacgta
       61 aaccgcagac tcctgtgcag cgcgtttctg tattatccac cgacgagaag gttgcattat
      121 ttcgtcggtt gtatcgtggg cgtgatgatg tatgggcact tagatgggaa agtaaaacca
      181 gcggcaaatc agggtactct cctgcctgcg ctaacgaatg gcaggcgaga atatgcggta
      241 aaccccggat aaaatgtggg gactgcgctc accgtcagtt gattcctgta tccgatctcg
      301 tcatctacca ccatctggcc ggtacccata ctgtcgggat gtatccgttg ctggaagatg
      361 attcctgtta ttttctggca gttgattttg atgaagctga atggcagaag gatgcatccg
      421 cattcatgcg atcctgtgat gagctgggtg taccggctgc gctggaaata tcccgttcac
      481 gtcagggggc gcatgtctgg atattttttg cctcacgagt ttcggcccgc gaagctcgcc
      541 gtctggggac tgctattatt agctatacgt gtagtcggac ccgacagctg cgattggggt
      601 cttatgaccg attatttcct aatcaggata ctatgccaaa agggggattt ggtaatctca
      661 tagcgttacc tctgcaaaaa agaccgcgtg aattgggggg aagcgttttt gttgatatga
      721 atctccagcc ttatcctgat cagtgggctt ttcttgtatc ggtgaccccg atgaatgtgc
      781 aggatattga accgacgata ttacgggcta cagggagtat ccatcctctg gatgtgaatt
      841 ttatcaacga agaagacctg ggtacgccgt gggaagagaa aaaatcatca ggaaacagac
      901 tgaatatttc tattgcagaa ccgctgaaaa tcacgctggc aaaccagatc tatttcgaaa
      961 aagcgcaatt acctcaggtg ctgattaacc gacttattcg gctggcagca tttccgaacc
     1021 ctgagtttta taaggctcag gcaatgcgta tgtcagtctg gaataagccc cgtgttatag
     1081 gttgtgcgga gaattacccg caacacattg cgttgccccg gggatgtctg gacagcgtat
     1141 tatctttcct tagggacaac aatattgctg cagaattaat cgataaacga tttgccggga
     1201 cggaatgtaa tgccgttttt atgggaaacc tcagagcgga gcaggaagag gccgtttcgg
     1261 cattactccg ttatgacact ggtgtgcttt gtgcgccaac ggcttttggt aagacagtta
     1321 ccgcagcggc agtgattgcc aagcggaaag tgaatacact gatactggta caccggactg
     1381 aattgctgaa gcagtggcag gagcgtctcg cggtgtttct gcaggccggt gacagtattg
     1441 gtattatcgg gggaggtaaa cataaaccct gtggcaatat tgatattgcg gtggtgcagt
     1501 ccatatccag acaaggagaa gttgaacctc tggtcaggaa ttatgggcaa atcattgtgg
     1561 atgagtgcca tcatattggc gcggtttcat tttctgcgat tctgaaggaa acgaatgcca
     1621 gatatctgct tggcctgacg gcaacaccaa tccgacggga tggtctgcat cccattattt
     1681 ttatgtactg tggtgccatt cgccatacag cggtccgccc gaaggaaagc ccacataatc
     1741 tggaggtact gatccgttcc cgttttacat ctggtcattt accatcggat gcgagaatcc
     1801 aggatatttt cagagaaatt gctctggatc atgacagaac ggtggcgata gctgaagaag
     1861 ccatgaaagc tttcgggcag gggcgaaaag ttctggtact gactgaacgt acagatcatc
     1921 tggatgagat agcatcagtg atgaattcac tgaaattgtc tccctttatt ctccatggtc
     1981 gactatcgaa aaaaaagcgt gcgatgctga tatccgggct gaatgctctt cctcccgatt
     2041 ctcctcgaat tttgttgtca acaggcagac ttattggtga gggatttgac caccctccgc
     2101 tggatacgct gattcttgcc atgcctgtgt catggaaagg gacattacag cagtatgcag
     2161 ggcgtcttca cagagagcat accggtaaaa gcgatgtcag gatcattgat tttgtggata
     2221 ccgcgtatcc tgtgctgctc agaatgtggg ataaacgtca gcggggttat aaagcgatgg
     2281 ggtacaggat tatagctgac ggcgatgaat cagtgattta attttaagga gtactttacc
     2341 ctttttaaat aatttgagct ccccgggttg ttcgggcaac aaagagtccc agtatacagg
     2401 cttcaggaat atgttgtagt atcaggcttt tcatcgcttt aaaatgacta ccggcagtga
     2461 gaacgtcatc aaaaataaca atctagggcc tgcacccttc cagtaattgt tcatccagtt
     2521 ggtagtttgc cataatcgct tctggtaagg gccggtttgt tgcggtatga gaggctattg
     2581 ttgatacgtt ttgcacaacc agttcccaga aatccagcca tggttcgtag gtttgcgcaa
     2641 ttcgcagaat ttctgtcata cggtcatcgt aagcggcatc tgtacggcat ttggatggtg
     2701 gtacgggaac aaatgtgtat tcctcaagat taccgatatt tgatatgagc atctgagcaa
     2761 catactcaat agcagccttt ttatagtggt aatcaggcta cgaaagtaca gcgttttata
     2821 aattgtctca ggtgatattc gcagcgtttt ttgacgtggt tttgttcgcc ttaaccatcc
     2881 tgatatttgc tctggagacc atttcatctc cagcttttcc agaacaagct ttcgcaatgg
     2941 taaattttga tccagtaagc acggttttgg ccttttcgcc attctgttgg ctcggttatt
     3001 agcatcaaca gctttgtaat agcgtctgcc ccgattac
//
LOCUS       DAZGXS010000261         3029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGXS010000261 DAZGXS010000000
VERSION     DAZGXS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(69..265)
                     /locus_tag="ACHKG5_004813"
                     /pseudo
     CDS             complement(69..265)
                     /locus_tag="ACHKG5_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(552..1370)
                     /locus_tag="ACHKG5_004814"
     CDS             complement(552..1370)
                     /locus_tag="ACHKG5_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0870735.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFLIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1522..1893)
                     /locus_tag="ACHKG5_004815"
     CDS             complement(1522..1893)
                     /locus_tag="ACHKG5_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0870736.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1883..2254)
                     /locus_tag="ACHKG5_004816"
     CDS             complement(1883..2254)
                     /locus_tag="ACHKG5_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0870737.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2267..>3029)
                     /locus_tag="ACHKG5_004817"
     CDS             complement(2267..>3029)
                     /locus_tag="ACHKG5_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0870738.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          926 a          775 c          580 g          748 t
ORIGIN      
        1 ataagcaaag gactgacagg tgccgctaac acccaccagc cgcccattta ccacaaataa
       61 aaaagccttc aggactgaag gcgtctgtaa caaccgaact gatagtctgc cagacccgca
      121 taaccagctg ggtcagtatt aactggcagc gctcgcgtga aaggtacgta ttctgcgcaa
      181 tctccccgac cgtcgccggt tcggtgacgc ttaattcatc aaacacaact ctggcggttt
      241 ctgtcatatc ctgctgtttc agcatgtctt tttacccttt ccggttaacg tgacacacca
      301 ataactcttg tcgaaaaagc cagcaagctg aaagacctgt attaataact accagcacat
      361 ttaacgcact gcgctacttt gcgggcacaa aaaacccgct cagaggcggg gtcaagctat
      421 gcggcgaaat aaccactctt aacagcatac ctgatttttt acgtacgtaa atgctttgcc
      481 gtgcatattt ttcatgcaaa tgtcacgtcc tgctattttt cagtcttata aatttaaaac
      541 catagaaaaa atcaattatg ttttaaaaat ggataggtaa agaataacaa gtgacacaga
      601 ttcaaaccaa aatggaaaag ggtggcaacc cacaaacgcc cactccacat ccatgacaat
      661 ccatacacaa caccagataa cgtggcaaat aatacaagta aagcaccacc tgaatagtga
      721 taaaaaccaa acaacaaagc cgccacaatt aatgcaacca atggagacgt tacttctgat
      781 agccgtgatt gaatataccc tctaaataat gattcctctg ccagagacac aaaaaacaaa
      841 ttagccaata taaactctgg caaccactca ggaaaatgaa tctctggctt taatccacca
      901 aaaaaaacag ccaaaaacag gataagagga acagagagcg acagagcccc ccacttccac
      961 aaagacactt ctgattttac ttctttttta aacaaagaag atgtacacag gactaacaaa
     1021 aatggcacca gcgctttatc aaaattaaaa tacattgtat agggagtact ttgagggcca
     1081 acagtaacag aatttagcac tacaggattg tgaaaccctg gccatagatg gaaaaataat
     1141 gctatggctg ataaaactat gccaacttca tatatagatt tagcccaggc gttatatttc
     1201 cagttgaact tcaaaataat taaaaaaacg attgtagcaa caaaaaataa caccgaccaa
     1261 tcaataatat cattaagcac agccaggaca acagacaccg tcaacaatga aaaagccact
     1321 accttatgcc aactgagagt tgacagtgac agtactaaca caatccacat aagcactcct
     1381 tttatttaat gatgaagatt gattatcaat attttcaatt caccaggcaa cattttatct
     1441 accttccaca atacatgacc atcaaaaaat aaacatgtta attataaaca cagaaaacat
     1501 aaccctcatc actatatatc cctaccgcat atccatatct aaccggacat tcagagccat
     1561 aagcattcct tcaataatac cttccgcttt ataaagcctt ttaccaataa gcccatcaga
     1621 acatctatgc ttacgtgcaa gagccataaa tgtcattcca cctacgtaat aatccaccaa
     1681 caaatcgtgc aaatactgat tattcttgtt taatcgtgcc atacaaccac aaattatcat
     1741 ggcgtcatca tcagaacact taggacgtga tttcacttta gtcgggatta atcctttaaa
     1801 accagcagct atcgagggcc atgttacatc ttcatgatta tttgctgccc atgcccccca
     1861 tcgctcaaga accatctgga tatcacgcgc catcgttacc acctctgatt tcgtaaatct
     1921 tcacgcccag ccgtccacct ggcacaggct gaccgcgcac aatattgatt tcatcaaact
     1981 gctcgtcatc aatgagcact tccgcatgcg tcagcgcatc cagcggtgct ttcagaatgt
     2041 tgtccaggtc acggcggcgc ttatccggtg gttctgcaat aatttttatt gccagccgtc
     2101 cggacaggct taatttcagc cgctgctggc gaacaataag cgccacagcc cggcgataac
     2161 gctcaccggc ttttgataca aaatatgtgc tgccacgacg tcgccagtag gtgttcaccg
     2221 tcggcgggta aggcaaaaca aactctatac gcatcagtaa cctcttttac ccgagcacgc
     2281 cggttgcaaa agcgtgatca agaaaacgaa aaattaaatc aacctgggaa ccatgctttt
     2341 cttcgaacgc cagcggatcc gcatgaagct cgttgtgatg ctcccgacac agcggtagcg
     2401 tgaaaatatc gtgggatttt gttcccattc caccctgacc atgaccaatc agatgatgag
     2461 gatcgtccgc tggcttacca caacacgcac acggctgtgt ctttacccag cgtgtgtatt
     2521 tctcatttac ccagcggcga cgtttaggtc gtttcatgaa ggatttcgga gactccggat
     2581 caacggcaat gctgaccacc gtcttttcct gtggtgggtt ctgttgctgg tgggtgtgag
     2641 gcggtagcgc aatatttttt gtgcgctgct tcagtatgct ggtggcggtc tgttctcccg
     2701 gtatgatgtc gctttcacgg tatacggagc ggattttttc cgccggtaat cccagagaac
     2761 gacgcgatac tgcctcaggt aatgcgtcca ccacctgatt gcagactgcc caccaggata
     2821 attcagccag cgataattcc cgttcctgta tgccattcat tgcgtggcgg atgacgtcaa
     2881 tcatccaggc ggccagattc tgttgagcaa gttgctcaag tgattcggat gtctggtcgc
     2941 gcagctggtt gtcgcagtgc cagcacaaca ccatcgcgcc ggtaccgtaa cgatgtatga
     3001 cagtttcgct gtgatgataa tcaccatga
//
LOCUS       DAZGXS010000262         3010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGXS010000262 DAZGXS010000000
VERSION     DAZGXS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            124..645
                     /locus_tag="ACHKG5_004818"
     CDS             124..645
                     /locus_tag="ACHKG5_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870739.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            1214..1396
                     /locus_tag="ACHKG5_004819"
     CDS             1214..1396
                     /locus_tag="ACHKG5_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870740.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            1374..1646
                     /locus_tag="ACHKG5_004820"
     CDS             1374..1646
                     /locus_tag="ACHKG5_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP0870741.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            1705..1956
                     /locus_tag="ACHKG5_004821"
     CDS             1705..1956
                     /locus_tag="ACHKG5_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870742.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            2139..2507
                     /locus_tag="ACHKG5_004822"
     CDS             2139..2507
                     /locus_tag="ACHKG5_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP0870743.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            2580..2744
                     /locus_tag="ACHKG5_004823"
     CDS             2580..2744
                     /locus_tag="ACHKG5_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP0870744.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            2713..2856
                     /gene="kil"
                     /locus_tag="ACHKG5_004824"
     CDS             2713..2856
                     /gene="kil"
                     /locus_tag="ACHKG5_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP0870745.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            2811..>3010
                     /locus_tag="ACHKG5_004825"
     CDS             2811..>3010
                     /locus_tag="ACHKG5_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0870746.1"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAY
                     YIQDRLEAQSWTRHYQQIAREE"
BASE COUNT          961 a          670 c          637 g          742 t
ORIGIN      
        1 gcagaggtgc ggcctttttt attgagagtg gatcttgaac ggaaattctc aagacttgag
       61 tcttgcatgc aaatcaattc ctggataaac tcgatctgag tcaataactt agagagagag
      121 caaatggcaa agtcaaacgt tagcgtgcag gcattcaagg acttccttga agagcttatg
      181 tcgctgaaca taatgaagga ggccaccgct cgaaatttaa aaaactcatc cgctcgcctc
      241 ttaacggtag tccaagaaga ggaaatgggt gatgttactc agcttgatgt gaatgagctt
      301 gccgagcgat acatcaacgc aactgagccg aagcctagcg acagcagcat tactgcatat
      361 aaaagccgca tggaaagtgc aatcaaaaag tttgtagctt tccagtctgg tgaagaaatc
      421 ccatacactc cgattgacaa agaatccagt gaggaaaaag atttgactgg cgaaccaaca
      481 aaagtcgaag gcaaggctaa tgcacttcat acctatgatc ttccagtagt tcttcgacct
      541 gaatcagggg ttacagtaac gattaaaggc attcctaacg atatcacaaa cgaagaagcc
      601 gaacgcatct cttcaattct gaaggtttac gttcggcctc aataatgcaa aagcattcag
      661 cacaatgtcc aactccccag tccgacattg atgctgttta accagagcct ccaaaaggag
      721 cccttgttaa ggtacacaat atttgcgatg taaccttagc gcgtctggta cattttttca
      781 agcggttgta gggctgccgc caatatgaga aaacacagat gtctacatac aatttgaatg
      841 accaattcga tcgagaagtt catgtgaatg cctatgaacg gataaggcat ggaaatcctg
      901 aacatgtgtg tgagcattat cgctcaaggc cacaccgcta atcatcaacc cggcctccat
      961 gccgggtttt cttttcctct cgcccaaaaa aacacataac caattgtatt tattggaaaa
     1021 taaatagata caactcacta aacaacgcaa ttctgatctc tccttacatc gccgaggcaa
     1081 tacacccacg ctaaaaaaca atactattaa atacaaagcg ttgtaaaaaa acgccccgtt
     1141 ttagaacaaa ttgtattgac caagtgaagt acatatcgta ctatttaacc atcagcagga
     1201 cgctggaagc caaatggaac agactggcag gctctttaaa caacgtcgac tctcgactac
     1261 gtggctgaaa agccagatca cccaaccaca taagctgtgg gatgcaatgc cgaagcaacc
     1321 gtctcaggag gagcttcgag attgcatcgc caaagtttat tcgggaggaa tctatgtcca
     1381 gaaaaacaga atttaaaggc accgcagctt ctcgccgtag agctcgtcgc gcaaacctgc
     1441 aaagtcagga ggcgattagc tccgacaaac tacacaggcc aaccccatca cgagtggtct
     1501 tacaatgcaa acgcaaacca gcaatgagag cagaagtaat aacactgaca acgttgacca
     1561 gaaaatatga aggctcaact tgtcttccaa atgtagctct ttacgcggca ggctaccgta
     1621 aatccaaaca actgacagca agatgacttg tgttggtcgc cagaaaatga aattaggcag
     1681 caaaccactt atttgaggtg atatatggaa tttcatgaaa gtgcgatttg tgattttcgc
     1741 gctaacgcaa attcagtaaa accacagcca attgcagttc tttttaaaac aatgggtgcg
     1801 tgggctgttt tatgcttcgc cgctgacgac actgacgcaa gaatggcaat aggacaagag
     1861 atggagatgg acccgacaaa cgatgaattc ataatttatg gcgctccatc taattactta
     1921 cttgatacct gcaacattta caacaaggct gcctgatggt ggcctttatt tttggcataa
     1981 acaacagaat aaacacagca ctgtgtattc attccaacga gtgaatacac ggagcaatgt
     2041 cgctcgtaac taaacaggag ccgacttgtt ctgattattg gaaatcttct ttgccctcca
     2101 atgtgagggc gattttttat ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
     2161 tcattgatta cgactggaaa gcatcaatag aaattgaaat cgaccatgac gtaatgacag
     2221 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
     2281 acggctcttt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
     2341 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
     2401 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
     2461 catcaggaat atttgattca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
     2521 gcataccaat aacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
     2581 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
     2641 gaatcacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
     2701 gagtcccaaa aaatggatca aacacttatg gctatccaga ctaaattcac tatcgccact
     2761 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
     2821 atactgaaac tgagatcaag caaaagcatt cactaacccc ctttcctgtt ttcctaatca
     2881 gcccggcatt tcgcgggcga tattttcaca gctatttcag gagttcagcc atgaacgctt
     2941 attacattca ggatcgtctt gaggctcaga gctggacgcg tcactaccag cagatcgccc
     3001 gtgaagagaa
//
LOCUS       DAZGXS010000263         2986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGXS010000263 DAZGXS010000000
VERSION     DAZGXS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            130..2340
                     /gene="katP"
                     /locus_tag="ACHKG5_004826"
     CDS             130..2340
                     /gene="katP"
                     /locus_tag="ACHKG5_004826"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP0870747.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2384..2773
                     /locus_tag="ACHKG5_004827"
     CDS             2384..2773
                     /locus_tag="ACHKG5_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0870748.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2830..2986
                     /locus_tag="ACHKG5_004828"
                     /pseudo
     CDS             2830..2986
                     /locus_tag="ACHKG5_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2886..>2986
                     /locus_tag="ACHKG5_004828"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          875 a          577 c          760 g          774 t
ORIGIN      
        1 ttgcggcttg gaacccctta gtattttttg tctgtagtat ctatcccagc aataggtata
       61 tcctgttgca tcaataaagt tgacttttgt atacaacatg cgaatttccc ttaatccgga
      121 gctattcgta tgataaaaaa aactcttcct gttctgattc ttctggcgct atcggggagc
      181 ttttctaccg ctgtagccgc tgataaaaaa gagactcaaa atttctacta tccagaaaca
      241 ctggatttaa ctcctctgag attacacagc cctgaatcaa atccctgggg ggctgatttt
      301 gattatgcca ccagatttca acagctggat atggaggctc tgaaaaaaga tatcaaagat
      361 ttgctgacaa cttcccagga ttggtggcct gcggattatg gtcattatgg tcctttcttt
      421 attcgtatgg cttggcacgg tgccggaaca tacaggacat atgatggccg gggaggcgcc
      481 agtggtggtc agcaacgttt tgaaccgctg aacagctggc cggataacgt taatctggat
      541 aaagcccgtc gattgctgtg gccagtcaag aaaaaatacg gctccagtat ttcctgggga
      601 gacctgatgg tcctgactgg taatgttgcc cttgaatcca tgggatttaa aacgctggga
      661 tttgctggcg gaagagaaga tgactgggag tcggacctgg tatactgggg gcctgacaac
      721 aagcctcttg cagataaccg ggataaaaac gggaaacttc agaaacctct tgccgccacg
      781 cagatgggac ttatttatgt caatcctgaa ggccccggtg gaaaaccaga tcctctggct
      841 tccgcgaaag atatcaggga agctttttca cgtatggcca tggatgatga ggagactgtg
      901 gccctgatcg cgggagggca tacatttggt aaagcacatg gtgcagcgtc tcctgaaaaa
      961 tgtattggcg cagggcctga tggtgcacct gtggaggagc agggactggg atggaaaaat
     1021 aaatgtggta caggaaacgg caaatatacc atcaccagtg gcctggaagg agcctggtcg
     1081 acatcgccaa cccagttcac aatgcagtat ctgaagaatt tatataaata tgaatgggag
     1141 ctgcacaaga gtcctgccgg tgcttatcag tggaagccta aaaaagcggc aaatatagtt
     1201 caggacgcgc atgatccgtc tgtcctgcat ccgttgatga tgtttacgac ggatattgct
     1261 cttaaagttg atcctgaata taagaaaata accacccgtt tcctgaatga tccaaaagct
     1321 tttgagcagg cattcgcaag agcatggttt aaactgaccc accgggatat gggaccggca
     1381 gcccgatatc ttggtaatga agttcctgca gaatcattta tctggcagga tcctcttcct
     1441 gcggcggatt atacaatgat tgatggtaaa gacattaagt cgctgaaaga gcaggttatg
     1501 gatttgggta tccctgcatc tgagctgata aagacagcct gggcttcagc ttccacattt
     1561 cgtgtgactg attatcgtgg gggaaataat ggtgcccgca tcaggttaca gcccgaaatt
     1621 aactgggaag ttaatgagcc tgaaaaactg aagaaagtac tggcatccct gacctcatta
     1681 cagcgtgaat ttaacaaaaa acagtctgac ggaaagaaag tgtcgttggc tgatttaatt
     1741 gttctttcgg gtaatgctgc aatcgaagat gcggccagaa aagccggggt ggaacttgag
     1801 attcccttta ctccgggaag aactgacgcc tctcaggagc agacggatgt tgcctcattc
     1861 agtgtactgg agccgacagc agatggattc agaaattatt actcaaaaag cagaagtcat
     1921 atatcgccgg ttgaaagcct cattgataaa gccagtcagc tggatctcac cgttcctgaa
     1981 atgacggcat tactgggtgg tctgcgggta atggatatta atacaaataa ttcttcgttg
     2041 ggagtgttta ccgatacccc tggtgttctg gataacaagt tttttgttaa tctgctggat
     2101 atgtcaacac gatggagtaa agcagataaa gaagatacat acaatggatt cgatcgtaaa
     2161 acgggagcat taaaatggaa agcatcctct gttgatttaa tcttcagttc aaatcctgaa
     2221 ttacgtgcgg tggcagaagt atatgcctcg gatgatgcga gaaataagtt tattcatgat
     2281 tttgttaaat cgtggaataa agttatgaat agcgatcggt ttgatttaaa caataaatga
     2341 taaaactgat gagggagggc acctccctca tgaaggagaa attatgcaga aaagcaataa
     2401 agttataatc agctgtggta tggcattata ttttttgtgt ggggggatta cagtggcaga
     2461 agaacttgat gaaagcatgc aagttcttgc aaggaatatg agtgtacttc agtcatcaat
     2521 ggataaaaag gagcttcttg aagctcttga cagaatggat gatgctgtag atgagtcaat
     2581 gaagcgtctt cctgagagca tttcaccggc tgatgaagaa gggaagaatg actatataaa
     2641 agaacttcaa aagcttgaaa aagagattat gctggcgaaa caaaaagtct tatcgggaca
     2701 gttatcagat atacccgatt cggtaaacaa gatgaataaa atcagagttg aggggcatga
     2761 taaattcaga taaatcagtt ggttgattcc tgcgggacga atacatacgt caggaacata
     2821 actttatggc tggaacgtga aaatcgtgaa ctgcgccgca gtaacgatat ccttcgccag
     2881 gcttccgctt attttgcgaa ggcggagttc gaccgcctct ggaaaaaata atgccactgc
     2941 tggataagct gcgtgagcag tacggggtcg gaccggtatg cagtga
//
LOCUS       DAZGXS010000264         2985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGXS010000264 DAZGXS010000000
VERSION     DAZGXS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            53..1264
                     /gene="bluF"
                     /locus_tag="ACHKG5_004829"
     CDS             53..1264
                     /gene="bluF"
                     /locus_tag="ACHKG5_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0870749.1"
                     /translation="MLTTLIYRSHIRDDEPVKKTEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGARQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1469..2200
                     /gene="bluR"
                     /locus_tag="ACHKG5_004830"
     CDS             1469..2200
                     /gene="bluR"
                     /locus_tag="ACHKG5_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0870750.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2421..2825
                     /gene="ycgX"
                     /locus_tag="ACHKG5_004831"
     CDS             2421..2825
                     /gene="ycgX"
                     /locus_tag="ACHKG5_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0870751.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKISTTKNPAKIKEAALLHICGKSTFQEYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2878..>2985
                     /locus_tag="ACHKG5_004832"
     CDS             2878..>2985
                     /locus_tag="ACHKG5_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870752.1"
                     /translation="MGIKVQRLSLWMINTALLLLPINNHQANTIKLIFEM"
BASE COUNT          878 a          625 c          640 g          842 t
ORIGIN      
        1 gatatgtctg ttaccgtctt actctcgcct ctcccattac cctggacagt taatgcttac
       61 cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa ccgaagaaat
      121 ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct tactgtttaa
      181 tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa tgatatatcg
      241 ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg attacgcgcc
      301 tgctcgccgt tttggcaaag cgggaatgga attatttgat ttacgcctgc acgagcgaga
      361 tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa cttatgatga
      421 cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa cctatttcga
      481 gattcctgct gaagactcct ggctttttat cgctgacgga tctgataaag aacttgattc
      541 ctgtaccctt tcaccaacta taaacgacca ctttgccttt catcctattg tcgatccctt
      601 atcgcggcgg ataatcgctt ttgaagccat tgtgcaaaaa aatgaagaca gcccgtcagc
      661 tatagcggtt ggtcagcgta aagacgggga aatctacaca gcggatctta aaagtaaggc
      721 gcttgcattc gcgatggcac acgcacttga gctcggtgat aaaatgatat caatcaatct
      781 attacctatg accctggtta acgagcctga cgcagtctct tttttacttg atgaaataaa
      841 ggccaatggt ctggtgcctg aacaaatcat cgttgaattt actgaaagtg aagtcatatc
      901 tcggtttgat gagtttgccg aagcggttaa atcgctaaag gctgccggta tcagtgtagc
      961 aattgatcat tttggcgcag gttttgctgg tctgttactc ctgtcacgct tccagcctga
     1021 cagaattaaa atcagtcagg aattgattac caatgttcat aaaagcggag cacggcaggc
     1081 aattattcag gcgatcataa aatgctgtac atcacttgaa attcaagtca gtgctatggg
     1141 cgtggcaaca ccagaagagt ggatgtggct tgaatccgca ggaattgaga tgtttcaggg
     1201 ggatctgttt gctaaagcta aattgaatgg tatcccttca gttgcgtggc cggagaaaaa
     1261 ataattttca acacattatt ttgcatgttt tctggaaatc atcgtgaaaa tgaatacgtt
     1321 atgttttaat ttaatccaca ttaaaaataa tgtttcatcc aatttagcta aaatcttatt
     1381 tatactttgt acaattttag cgtatattgt acaaagtatt attgggtcgt atacaggcga
     1441 cggagattta tgaccgcaag gaggaattgt ggcttattac agcattggtg atgttgctga
     1501 acgttgcggg attaatcctg tcactctccg ggcctggcaa cgccgctacg gtttgttaaa
     1561 accacagcgc agtgaaggcg gacaccgact ctttgatgaa gaagacatac aacgtatcga
     1621 agagatcaag cgttggataa gtaatggcgt ccctgtaggc aaagttaaag cattactgga
     1681 aaccaccagc caggatacgg aagatgactg gagccgcttg caagaagaga tgatgtcaat
     1741 tcttcgtatg gctaatcctg ccaaactacg cgcgagaatt atttcactgg gtcgagagta
     1801 cccagtagat caattgatta atcatgttta ccttcctgtt cgccagcgtc tcgtgcttga
     1861 tcacaacacc tcccgcatta tgagcagtat gtttgacggc gcattaattg aatatgcagc
     1921 agcctcgctt ttcgaaatgc gccgtaagcc cggtaaagaa gccattctga tggcgtggaa
     1981 tgttgaagag agagctcgat tgtggctgga agcatggcgt ttatcattgt caggatggca
     2041 tatttctgtt cttgctgatc ccattgaatc accgcgcccg gaactgttcc cgacgcaaac
     2101 attgattgtc tggacaggca tggcaccaac gagaaggcaa aatgaacttt tacaacactg
     2161 gggtgagcaa ggttataaag tcatctttca tgccccctaa tttcaccttt atcccgcccc
     2221 ttagacagaa tgttataatc tgaacccaca acagcgtttg taccggagct ggtccattac
     2281 aaaatgctca ccgggagaat tcaggtaatc cccctttcac actttgtaca tatcacagcc
     2341 aaaggacaga ccagattgaa gtggtaaaac cgatcgtgca cactacgcag tctcacaact
     2401 aacaagaggt tattatcctg atggatcagg tcgttgtttt tcaaaagatg tttgagcaag
     2461 tgagaaaaga gcaaaacttc tcctggtttt attcagaatt aaaacatcac cgtattgcac
     2521 attacattta ttatctggct acggataaca tcagaattat tactcacgat gacacggttt
     2581 tgttattaag aggaaccagg aacctgttaa aaattagtac gaccaagaac cctgctaaaa
     2641 taaaagaggc cgcattgctt catatttgcg gaaaatctac atttcaggaa tactgttcaa
     2701 cactggcagg cgcgggcgtt ttccgttggg ttactgatgt taatcataac aaacgcagtt
     2761 actacgccat tgataatacg cttttatata ttgaagatgt agaaaataat aaaccattaa
     2821 tctagctaaa gttggatgct taagaaatgc ttcataattc agtaagacat tagcataatg
     2881 ggaataaaag tacagagact atccctatgg atgataaata ctgccctttt attgctaccc
     2941 ataaataatc accaggccaa tactatcaaa ttgatatttg aaatg
//
LOCUS       DAZGXS010000265         2978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGXS010000265 DAZGXS010000000
VERSION     DAZGXS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            191..1144
                     /gene="ompT"
                     /locus_tag="ACHKG5_004833"
     CDS             191..1144
                     /gene="ompT"
                     /locus_tag="ACHKG5_004833"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0870753.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1331..2815
                     /locus_tag="ACHKG5_004834"
     CDS             1331..2815
                     /locus_tag="ACHKG5_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0870754.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          975 a          626 c          585 g          792 t
ORIGIN      
        1 aaaatttcca gcatattaaa ccacaacaat aatacaacac ttggcaagtt gcccataaag
       61 aatgaataaa acgaaacaat aattcaaact taaacaaacc ataaacagtt aaacaatata
      121 caattgactc attaagttag atataaaaaa tacatattca atcattaaaa cgattgaatg
      181 gagacctttt atgcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag
      241 ctcttttgct tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag
      301 tcttggaact ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg
      361 aaaagtcagt caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa
      421 ttgggatttg atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg
      481 aggtggcaat atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga
      541 tgaaagtaga caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa
      601 aggctggctc ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag
      661 ccgttatagc tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag
      721 agatgatatc ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa
      781 aatgccctac attggcttga ctggaagtta tcgttatgaa gattttgagc taggtggcac
      841 atttaaatac agcggctggg tggaagcatc tgataacgat gagcactatg acccaggaaa
      901 aagaatcact tatcgcagta aagtcaaaga ccaaaattac tattctgttt cagtcaatgc
      961 aggttattac gtaacaccta acgcaaaagt ttatgttgaa ggcacatgga atcgggttac
     1021 gaataaaaaa ggtaatactt cactttatga tcacaatgat aacacttcag actacagcaa
     1081 aaatggtgca ggcatagaaa actataactt catcactact gctggtctta agtacacctt
     1141 ttaacgaagt taaccagatt ttctccccgg tggtattgta taaccccggg gaatttgttt
     1201 acagttacaa gtagacaata tccactaatt ttgtaagcat ttaacctatg tcataacgtg
     1261 taatctctta ccgcaacaca agacctcaat atattcaaat tttatcttta ttccgatatt
     1321 cagagacaca atgcgaaaaa taattactca tttcaaagtt gttttaacgt tacttctacc
     1381 agtaaccgta tctgcccagc agatacagtg gcaatcctgt atggccagtc aattcaacca
     1441 ctggtttggt gaggaaaaac cgtctcctga cttactatgt ggttatttgt ctgttccatt
     1501 aaaatataca gacacaggcg gagatgcttc ttatgaaaaa aaatcacaag tcaaactagc
     1561 gttgacaaaa ttgccggcaa aaagcaagca taaaggaagt atcctgataa taagtggtgg
     1621 tcccgggtta ccaggcataa atccttatat taactttgac tggccagtca caaatcttcg
     1681 tgagtcatgg gatattattg gatttgatcc tcgaggcgtc gggcagtcca ctccgacaat
     1741 aaactgccgg caatcagata cagagactca ggaaaacata accgaaaagc aacaagtatt
     1801 aaataaaatt aatgcctgta tccataatac cggagccgaa gtcattcgcc atatcggctc
     1861 taacgaggct gtatacgata ttgatcgtat taggcaagcc ttgggggata aacaactgac
     1921 agccgtggcg tattcgtatg gaactcaaat tgcagcctta tatgcagaac gttttcccta
     1981 caacgtaaga tctatcgttc ttgatggagt cgtcgatatc gatgacctgg aggacaactt
     2041 cacatggcaa ctcaaacagg cacagagtta tcaggaaacg tttgatcgct ttgcatcctg
     2101 gtgtgcgcgt acaaaaagtt gcccgctttc ttcagacaga gataaggcaa taactcagtt
     2161 ccatgagcta ttatcaaaat tacatcacaa acctttatta gacagtaagg gagaaaatat
     2221 atcttcagat gaactcatat cattaacaac agaccttctg ctatggcgtt catcatggcc
     2281 aacccttgca actgccatac gccagttctc tcaggggatt gtcagtaatg aaattgaaac
     2341 tgcgctcagt gctccgatag cctcagaaga gtcaagtgat gcttcggggg taatcctttg
     2401 tgtagatcag ggggatgagc aattaacacc agaagagcga aaattccgaa aagaggctct
     2461 tgcgaatgcc ttcccggcta ttaactttga caatggacgt tccgattcac ctgatttttg
     2521 tgaattatgg ccaatacata gcgacctgaa caaaactcgc ctgaaaaata ctgttctgcc
     2581 ctctggttta ctgtttgtag cacacaaata cgacccaaca acgccctgga ttaatgcccg
     2641 taagatggca gagaaatttt ccagcccgtt actaacaata aatggtgatg ggcatacatt
     2701 agctctcacc ggagttaatt tatgtgtaga taaagcagtt gtacatcacc tgatcactcc
     2761 acaaaaaata gaaaatatat actgcccagg aaattctgaa gcagaaatac aataatttca
     2821 aaatattccg ctatttgctc ccaatgcaaa acatatattg cattgggagc atattcatat
     2881 ttttgttatt cttgtatgaa atcgtctcat gtacacggtc cttttttcgc cgaaaactga
     2941 tattacttta atcaaaagtt gacgtgccag attcgaag
//
LOCUS       DAZGXS010000266         2977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGXS010000266 DAZGXS010000000
VERSION     DAZGXS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..209
                     /locus_tag="ACHKG5_004835"
     CDS             <1..209
                     /locus_tag="ACHKG5_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0870755.1"
                     /translation="RRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIESCRRELAA
                     DIKYARQLTIKKNDTQYALRWLFI"
     gene            complement(672..929)
                     /locus_tag="ACHKG5_004836"
     CDS             complement(672..929)
                     /locus_tag="ACHKG5_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870756.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(926..1423)
                     /locus_tag="ACHKG5_004837"
     CDS             complement(926..1423)
                     /locus_tag="ACHKG5_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0870757.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1626..2063)
                     /locus_tag="ACHKG5_004838"
     CDS             complement(1626..2063)
                     /locus_tag="ACHKG5_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0870758.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(2060..2557)
                     /gene="rrrD"
                     /locus_tag="ACHKG5_004839"
     CDS             complement(2060..2557)
                     /gene="rrrD"
                     /locus_tag="ACHKG5_004839"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0870759.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2557..2772)
                     /locus_tag="ACHKG5_004840"
     CDS             complement(2557..2772)
                     /locus_tag="ACHKG5_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0870760.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2849..>2977)
                     /locus_tag="ACHKG5_004841"
     CDS             complement(2849..>2977)
                     /locus_tag="ACHKG5_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870761.1"
                     /translation="LLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT          726 a          784 c          678 g          789 t
ORIGIN      
        1 cccgcagaac aacaacccgc aacatccgat ttccaaacca gatgattgaa caaattaaca
       61 tcgctcttga ccagaaaggt tcagaaaatt tttctgcgtg ggtcattgaa tcttgccgcc
      121 gggagctggc agcagacata aaatatgccc gtcagttgac tataaaaaag aatgatacac
      181 agtatgctct gcgatggctg ttcatataac tatttcttta tattgctgaa tttataaaaa
      241 ctcacagaca ttagctgtat taattccgaa ttgaaataat cagccatata gaataaaaat
      301 aaagcataac aataataatc ttctacccaa tcagtacatt actgctgtga ctccaacacg
      361 gcagtttttt tattgaacag attccagttt cttccaccat cgcaccggac gggcgaccat
      421 gaggggagaa cgccgcgctc cgtttacgcg gtaaaccccg gtgtgtatcg tttttgatta
      481 tccccgcaca ctcgcgcaga aggaattccc cgtcgggcta cggtcatggt taatgcggaa
      541 atacagcgac gatacagcgc atgatgtgtc aggcttgaat acctttatcc gttaaaaggg
      601 atatcagtta agttatcccg tgtagggtat aagccattat caagcccacc agtagatggg
      661 ctttgtaatg gctacttcgc ttttgcttcc gctcgcttac gccggcgctc ttctttcctc
      721 tcggcttttg ccatgtccat gaatgcctgc atgatcgagt tccgcatcat gtagctaaca
      781 aagtgatgat tgacacagcc gttgaggcgc agctgctcgc caaactcatc caccgaggcc
      841 aatgcttcca tcataccctt ctcgcctttc atgaactctg agaagtcgcg ccccgctctg
      901 gaggcgcatt caatgacacg atcactcatc ccggaagccc ggggatcgaa atctgcaact
      961 ggttagccag ggagttaatt tcagcgatca aaccaggttt cgtgtagcgc catgccgcga
     1021 ggccttgtcc acagaagctc gccatgtctt ttttctgatc aaactcatga catttcatgt
     1081 tgagctgcgc acttaagctg ttccgatgat gaagctctcc ggtgaagtaa tcatcgagga
     1141 ctttataggc tgcatattta aatccggggt ttagccatgc tgcgtaatca taagcaacga
     1201 actttccgcc ataagttcca ccgtgcgcac cgcgttccgt aaaaaccaca gattcgtggt
     1261 ttttctccag ctcagccaag aactctttgg tctgcttgtt tcgcaggtag tggtacggag
     1321 attcagattc acttttacca ctggctttcc acatatcagt gaggcagatc atgccatctt
     1381 caccgatacg gattggttga ttgaagaggg ttaatgattt catagcgtgt acctactctt
     1441 tgaaatgaac ctttgccgca caggaaacca gcccaccgag gctcgccagc actaactggt
     1501 atcctcaaag gcccattcca aaggggcagg ttcggtgtaa aaaacatgcg ttgcggtacg
     1561 catttattgc aaaaagcccc gcatcgcgag gctcattaaa tggactttgt gatttgcaaa
     1621 aaaaattatt tcaggcattg cgtcctgatg tactcctgca ggtagttaac ctgcgcggtt
     1681 atcttgtcga ttccacctcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct
     1741 gggctttctc catcgcccat gctgctggct ccggtcgttg actttgcaca ggtggcggag
     1801 acttgcaggc gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcatta
     1861 gcatcagcaa gctcctttgt gtatctggcg tcaagttctg ctacatcacg ttgccgcttc
     1921 tgcatatcag cgatgatgga tgtggcttta tcgcgctgct ctttgtaggc gatggcgtta
     1981 tcacggtaat gattaacagc ccatgacagg caggcgataa tgcagataac cagagcggag
     2041 ataataacgg ttaccctgct cattgttgcc cccacaaaca gacttcacgc tcaatctcgc
     2101 ggcgagtcat cagccctttc cattgcttac cgccagcgta tgtccagcgc cgtagctgat
     2161 cacatgcgcc tttgatatcg ccctggttta ttttgcgaag aagcgtcgat gttctgaaat
     2221 tgccagcgcc cacgttgtaa acgaacgagt aaagagcgcc gcgcgttgtt tccggtatat
     2281 cgacgttgat gtacgggtta atttgtctgg cgaccgtggc aaggtcttta ttcaggaggg
     2341 ctttgcattc tgcttcggta tacgttttac cgggcatgat gtcttttccg gtgtgtccgt
     2401 gacatacagt ccatacgcca acgatatctt tgtatggtat gtagctgaca ccttccagac
     2461 catcgttacc acctggacca gtgatgagca cagacgctat ggcaacagcc ccaccaccaa
     2521 tagcagctgc aacagccttg cgtaatgacg gcgacattat tcacctctcg cagccttacg
     2581 cttatcttct ttaatcttga aataaagatt tgtcagatac gtcagcaggc caaacagcag
     2641 acttcccagc acacctattg ccacccactg ggacggagag actttgtcca gcagctgcag
     2701 taaccagtat cccgtcccca ccgctgacgt ggtgtatgac acacctgttg tgattttttc
     2761 catctggtac ataccccgtc tcccgttatc cggaagctca caacatgaaa aaggccagca
     2821 gctgtttact gatggccctg actccccgtt acagcatcat gaccgattcg ggttgaggtt
     2881 cagtcgcatc ggcgaccggt gattcaggct gaacttcacc gctctctgcg gtggtatctc
     2941 ccgcttcagt cggtggctct gcctgtacac caagcag
//
LOCUS       DAZGXS010000267         2897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGXS010000267 DAZGXS010000000
VERSION     DAZGXS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            30..1967
                     /locus_tag="ACHKG5_004842"
     CDS             30..1967
                     /locus_tag="ACHKG5_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0870762.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPGTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTY
                     DRPDPRIKQLARRSTVTPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLAD
                     MVGQCAGGSADGVPWICGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     ANVPTNKPEEDPDIPASGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILL
                     HAGRTAELVGGEQVVMPPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWG
                     AEGGRALVVDDAGAAGGKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDG
                     TLTANQYALALYWPVSSLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNT
                     KLGSFGAFDNEWHTLGFRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTD
                     ITSGATYPVLIESITVEVNNP"
     gene            2115..2297
                     /locus_tag="ACHKG5_004843"
     CDS             2115..2297
                     /locus_tag="ACHKG5_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0870763.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2335..2604
                     /locus_tag="ACHKG5_004844"
     CDS             2335..2604
                     /locus_tag="ACHKG5_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0870764.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2680..2895
                     /locus_tag="ACHKG5_004845"
     CDS             2680..2895
                     /locus_tag="ACHKG5_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0870765.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          719 a          677 c          870 g          631 t
ORIGIN      
        1 actctgttcc tgtacacggg agaaattcga tgtcgattaa acattatgat gttgtcaggg
       61 cggcgtcgcc gtcagatctt gcggaaaagc tgacacacaa actgaaagag ggctggcagc
      121 cgtttggtag tcctgtggcc ataacccctt ataccctgat gcaggcgatt gccgcggagg
      181 gggatgtgac cacgccagtg gttgtgcccg gcacggggga tggtggctat ccgggagtgg
      241 tcaccacgga gccagattat tactacgtta ttccactggc cgggcagtcg aacggcatgg
      301 cttacggtga ggggcttcct ctgccgcaga catatgaccg tcctgacccg cgtataaagc
      361 aactggctcg tcgcagcaca gtgacgccgg atggcgctcc ctgtaaatat aacgacatta
      421 ttccggcaga ccactgtctg catgatgtac aggacatgag ccgtcttaac catccgaaag
      481 ctgacctgtc gaaaggtcag tacggaaccg tggggcaggg gctgcatatt gccaaaaagc
      541 tgctgccgtt tataccggcg aatgcgggta ttcttctggt tccgtgctgc cgtggtggtt
      601 cagctttcac caccggggca gatggaacat acagtgacgt gaccggtgcc tcagagagtt
      661 ctacccgctg gggtgtgggc aggccgctgt ataaggatct catcggtcgt acaaaagccg
      721 cgctggcaaa gaacccgaaa aatgtgctgc ttgccgtggt gtggatgcag ggggaatttg
      781 actttgacgg aacgccagca aatcacacag cccgttttac agaagtagtg gaacaatatc
      841 gtacggacct tgcagatatg gtgggacagt gcgctggtgg ttctgctgac ggtgttccct
      901 ggatatgtgg agacacaact tatttctgga agcagaagag cgaatccact taccagacgg
      961 tgtacggcag ttacaaaaac aaaacggaaa agaatattca ctttgtgccg ttcatgaccg
     1021 atgagaacgg agcaaatgtc ccgacgaaca aaccggaaga agacccggat attccggcat
     1081 caggatatta cggtgcggcc tcccggacgt cggcaaactg gacgtcagca gaccgtgcga
     1141 gccatttcag ctcatgggca cgcaggggga ttatttctga ccgtcttgcc tcagcgattc
     1201 ttctccatgc aggacggacg gctgaactgg tgggtgggga acaggttgtg atgccgccgg
     1261 atgagaagcc gtcaccggac acaccatcaa caccgtcaac ggacgggaaa tcagtgacaa
     1321 cgctgcttta ttaccgtgca acagagtcag gtggtttact gaatccgcag ggatggggag
     1381 ctgaaggagg gcgtgcattg gtagttgatg atgcaggtgc tgcaggaggt aaggcgctga
     1441 ggtggaccaa acagacagga agttcctcgt ggtttatgca gcatgatgcc ggtaatggcg
     1501 cagacctgct ggagaagggc gggcttatca gttgtcgttt taaagttgat ggcacactga
     1561 cagctaatca gtacgcactg gcgctgtact ggccggtttc ttcactgcct cagggcgtca
     1621 cactggaagg taatgccggt cataacctgc tggcgtcgtt ttacgtacag agcgatgcca
     1681 cagaccttaa tgtgatgtac cacaagggaa atgctggtca gaacacgaag ctggggtcat
     1741 tcggcgcatt tgataacgaa tggcatacgc tgggcttccg ttttgccggt aacaacagta
     1801 ttgaggtgac gccggtcatt gatggtaagg acgggacgcc gttcatgctg tcacagtcac
     1861 cggtcggcac gtttacggca gacaaattgc gcgtgaccga tatcactagc ggtgcgacat
     1921 atccggtgct gattgaaagt ataacagtgg aagtgaataa cccgtaagca ggaaaaaaag
     1981 gccgccgggg cagggaaaac aaggagccag aaccggcggc aaatgtcgtt atatccaaag
     2041 caaaacatgc aggacacttt tttaaccaac aggtattaac gatgtcaaca ccatatcaat
     2101 aaccgggagg gataatgaga tttgtacagc ttattttatt gtatttctgc acggtggtgt
     2161 gcacgttata tctggtaagt ggcgggtata aggttatccg gaactatata cgcaaaaaga
     2221 ttgatgccgc ggcggcggaa aaaatcagcg ccagccagtc agccggaaca aaacccgaag
     2281 agcctctcat ttcgtagcaa ctttcttaac aacacctttc aacgagaaaa tcccatgtca
     2341 gaaataaaat ctctggtcac tgctgaagca gtgaaggacg tcctgcgctc tgaagaagtc
     2401 agaagcgcac tgaaacagca actccgccag aatcttgagg cgcgtcttga tgctgaagtg
     2461 gatgccattc tggatgagct gctgggggga ccggctgctc ctgagcctga agacggcgcg
     2521 ggtgacagtg ctgtttcaga tggcgttgtg tctcagcctg acggtagcag tgagcctcag
     2581 cctggcggcg aaatgatgat gtaaccatac gcaggggctg tcggtgtgag ctgatgcccc
     2641 tcctgttgtt gtgagcttcc ggattgcggg agacggggta tgtaccagat ggaaaaaatc
     2701 acaacgggtg tgtcatacac cacgtcagcg gtggggacgg gatactggtt actgcagctg
     2761 ctggacaaag tctctccgtc ccagtgggtg gcgataggtg tgctggggag tctgctgttt
     2821 ggcctgctga cgtatctgac taacctgtat ttcaaaatca gagaggaccg tcgtaaggcg
     2881 gcgcggggag agtaaag
//
LOCUS       DAZGXS010000268         2885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGXS010000268 DAZGXS010000000
VERSION     DAZGXS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..905
                     /locus_tag="ACHKG5_004846"
     CDS             <1..905
                     /locus_tag="ACHKG5_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0870766.1"
                     /translation="ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLL
                     RTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWAD
                     IVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLD
                     DGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAKHSVSELPVNW
                     WVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARIRENITLGVQA
                     GYAHSVSGSSAEGYNGQATLNMTF"
     gene            1016..>2885
                     /locus_tag="ACHKG5_004847"
     CDS             1016..>2885
                     /locus_tag="ACHKG5_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP0870767.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDE"
BASE COUNT          748 a          728 c          822 g          587 t
ORIGIN      
        1 gtgaaaataa cagcgtccgt ctcagcattc agggcggtca tctcggtcac gataacaacg
       61 gcggtattgc ccgtggagcc acgccggaaa gcagcggcag ctatggcttc gtccgtctgg
      121 agggtgacct gctcagaaca gaggttgccg gtatgtctct gacgacaggg gtgtatggtg
      181 ctgcaggcca ttcttccgtt gatgttaagg atgatgacgg ttcccgcgcc ggcacggtcc
      241 gggatgatgc cggcagtctg ggcggatacc tgaatctggt acacacatcc tccggcctgt
      301 gggctgacat tgtggcccag ggaacccgtc acagcatgaa agcgtcatcg gacaataacg
      361 acttccgcgc ccggggctgg ggctggctgg gctcactgga aaccggtctg cccttcagta
      421 tcactgacaa tctgatgctg gagccacaac tgcagtacac ctggcaggga ctctccctgg
      481 atgacggcca ggataacgcc ggttatgtga agttcgggca tggcagtgca caacatgtgc
      541 gtgccggttt ccgtctgggc agccacaacg atatgacctt tggtgaaggc acctcatccc
      601 gtgacaccct gcgcgacagt gcaaaacaca gtgtgagtga actgccggtg aactggtggg
      661 tacagccttc tgttatccgc accttcagct cccggggtga catgagcatg gggacagccg
      721 cagccggcag taacatgacg ttctcaccgt cccggaatgg cacgtcactg gacctgcagg
      781 ccggactgga agcccgtatc cgggaaaata tcaccctggg cgttcaggcc ggttatgccc
      841 acagcgtcag cggcagcagc gctgaaggct ataacggtca ggctacgctg aatatgactt
      901 tctgattata cggtatcccg gaaatacgat ttcaggaaaa ttcatgcagt aatgggaaag
      961 gacgctcatt gctgttcacc gcagttctga attattgaat ttataaaggt gaatgatgca
     1021 cgaaaagaac atcgccctgc tttgtgatga agccgaccgg cttttgcaac tgaatatcaa
     1081 tctgctccgg caaatggttg atgagccgga tgtgttatct gacagtaaga acgaaaacgg
     1141 gctgcttttt gataaacgga aagcgctgaa aagaattgag gagctggagg gcgaacaaat
     1201 caaaaccgcc cgcagggaga tggtgctggc tgtggtcggc acgatgaagg cgggcaaatc
     1261 aaccaccatc aacgccattg tggggcagga aattctgccc aaccgtaacc gccccatgac
     1321 ctcggtaccg acattgatcc gccacgttcc cggaaaaacg gaaccggtcc tccatctgga
     1381 acatattcag cccgtccgca atttattaat cacactgcag gaaaaactcg ccaccccggc
     1441 agggcagcaa gtcgcacagt ccctgcagca aaccggggat acccgcgaac tactggatat
     1501 tctggcggat gatgtctggc ttaaaaatga ataccacggg gaagacgaaa tctttaccgg
     1561 actggcatcg ttaaatgacc tggttcgtct tgctgcggca atggggactg aatttccttt
     1621 tgacgaatac gcagaagtgc agaaactacc ggtgatcgac gtggcgttca gccacctggt
     1681 gggaatggat gcatgtcagg gaacactcac actgctggat acccccggcc ctaatgaagc
     1741 cggacaacca cagatggaaa tgatgatgcg ggatcaacta cagaaagcct ctgcggtgct
     1801 ggcggtgatg gattacaccc agatgaactc gaaagccgat gaggaggttc gtaaagagct
     1861 taatgccatt gctgacgtct cagccggtcg tctgtttgtg ctggtcaata aattcgatga
     1921 aaaagatcgc aatggtgatg gtgcggatgc cgtacgccag aaggtgccag caatgctgaa
     1981 cagcgatgtg ctgcccgcct cccgcgttta tcccggctca tcacgtcagg cgtacctggc
     2041 taaccgtgcg cttcatgagt tacgaaaaaa cggtgcgctt ccggttgatg aagcctgggt
     2101 cgatgatttt atcagggaag ctttcggacc tatggtggaa gaggacgact ggaaagacag
     2161 cactaaggta aataaaaagg cagaaaaact ctggaatata tctctgatcg atcaactgat
     2221 aacggaagtc atacagagct cccattccag agcggcggca ctggcggttg actctgccgc
     2281 cgcaaaactg atgcagaatg cagaaaatgt cagcgaatat ctgtcattac gtcatcaggg
     2341 gctgcagcaa agcattcagt cactacaggc gcatattacc agcctgctgg cggatatcca
     2401 ggagattgag gaatgtcaga atcaggtgac cggtgatgtc agaatggcca tggaggacat
     2461 caataccaaa accggggaat tactgacgaa ggtctgtgcc tcactggagg aggagctgaa
     2521 cgactatttc agaagcggta aacgcaaaga acagcaaatg ctggaggaag aaaattcagc
     2581 gcagccccgg gagagaaacg cattcgcttt ctttcacgat attttcggaa ccggaaatca
     2641 gcatgacagg atgcgggact ttgatccgga cagccccgaa ataaaattca gtgatcgcag
     2701 ggcagccctt gaactgatga cgcaaatcga atcgaccgtg accagcctgc accgtgaggc
     2761 tgaagcacag ttccggcctg agctggagaa aatcgtcagc gggattgaaa caggttttcg
     2821 tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc atcaatgccc gcctggagga
     2881 tgagg
//
LOCUS       DAZGXS010000269         2870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGXS010000269 DAZGXS010000000
VERSION     DAZGXS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            152..2431
                     /gene="ydeP"
                     /locus_tag="ACHKG5_004848"
     CDS             152..2431
                     /gene="ydeP"
                     /locus_tag="ACHKG5_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0870768.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2679..>2870
                     /gene="safA"
                     /locus_tag="ACHKG5_004849"
     CDS             2679..>2870
                     /gene="safA"
                     /locus_tag="ACHKG5_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0870769.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSLQ"
BASE COUNT          774 a          658 c          691 g          747 t
ORIGIN      
        1 tttaatctat tgtttaacga taatagatta aaaatagaaa gagagtagtc ccctactctt
       61 atatatccat gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc
      121 ctaataattc atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg
      181 tgctgccggt ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga
      241 tatacgccag gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc
      301 gggttgtgca tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc
      361 aaaagcaatc gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctcagaa
      421 tacggttcaa tcattactta cctggggaga tcacgagctt gaggctgcgg gacgtctcac
      481 tcagcctttg aaatatgatg ctgtcagcga ctgttacaag ccattaagct ggcaacaagc
      541 tttcgacgaa attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta
      601 tacttcgggc cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata
      661 cgggagcaat aactttcccg actgctccaa catgtgccat gaaccgacaa gcgtgggttt
      721 ggcagcgagt atcggtgtag gtaaagggac cgtgttgctg gaagactttg agaagtgcga
      781 tttagtcatt tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt
      841 gcgcgcttta gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg
      901 cctggagcga tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca
      961 gttggccagt gcctactata acgtgcgcat tggtggtgat atggcgttgc tcaaggggat
     1021 gatgcgcctg ttaattgagc gcgatgatgc tgcaagcgcc gcaggtcggc cctcattgct
     1081 ggatgacgaa tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgtgct
     1141 caattccgag tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga
     1201 actggctgat gcatatgccg ctgccgaacg aaccattatc tgttacggaa tggggatcac
     1261 tcagcacgaa catggtaccc agaatgtaca gcaactggtc aatctgctgt tgatgaaagg
     1321 taacattggc aagcctggtg cgggtatctg cccactacgt ggccactcta atgtacaggg
     1381 cgaccgaacc gtcggtatca ccgagaaacc gtctgtagag tttctggctc gcctgggtga
     1441 gcgctatggc ttcaccccac ctcatgtacc tggacatgct gcaattgcca gcatgcaagc
     1501 aatatgtacg gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat
     1561 gccagatcgg gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc
     1621 cactaagctt aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct
     1681 gggacgtagc gagattgaca tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc
     1741 aatgtcgatg attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc
     1801 agagtgtgct gtggtcgcgg gaatcgcgca ggcaacacta ccccagagcg tggtagcctg
     1861 ggagtatctg gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga
     1921 gttcgccgac tataaccaac gcatccgtca tcccggtggt tttcacctga taaatgcagc
     1981 tgctgaaagg cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct
     2041 gttagaagat ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca
     2101 cgatcagtac aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca
     2161 acgagatgtg gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga
     2221 aagagttaat cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag
     2281 attaaaagtg gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc
     2341 gaatcacatg ctaacacttg ataaccacga tccattaagt ggcattcctg gctataaaag
     2401 tattccggtt gaattagaac catcaaatta atgcctcttc tcatttcttc tgctgtcatc
     2461 cgcacagcag aagaattcct cattgattat tatcgcgcaa tttgctcaca tggattaaat
     2521 taaactacat actataagat ataaacttct gcctacagct gtaagaaact ccgctcagta
     2581 ctgaagcacc agtcctattt cctcttttct caagcctgtt atattaagca tactgattaa
     2641 cgatttttaa cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa
     2701 acaaaatcac gcaaagagac aactataaag aaatcatgtc tgtaattgtg gttatcttat
     2761 tactgaccct tacgttgata gccatttttt cggcaattga tcagctgggt atttcagaaa
     2821 tgggtcgcat tgcaagagat cttacacatt tcattgtcaa tagtttgcaa
//
LOCUS       DAZGXS010000270         2851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGXS010000270 DAZGXS010000000
VERSION     DAZGXS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(437..613)
                     /locus_tag="ACHKG5_004850"
     CDS             complement(437..613)
                     /locus_tag="ACHKG5_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870770.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            788..991
                     /locus_tag="ACHKG5_004851"
     CDS             788..991
                     /locus_tag="ACHKG5_004851"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFP0870771.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(923..1387)
                     /locus_tag="ACHKG5_004852"
     CDS             complement(923..1387)
                     /locus_tag="ACHKG5_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870772.1"
                     /translation="MPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGTFFS
                     LVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRTVTE
                     VNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1316..2056
                     /locus_tag="ACHKG5_004853"
     CDS             1316..2056
                     /locus_tag="ACHKG5_004853"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP0870773.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2290..2851
                     /locus_tag="ACHKG5_004854"
                     /pseudo
     CDS             2290..2851
                     /locus_tag="ACHKG5_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          771 a          699 c          662 g          719 t
ORIGIN      
        1 atttgaacac actgacaaaa aaccaagcat ggtaataaac cgaactcccc acaaacttct
       61 gttgcctcag atactccccc ccctttcctc aggcacaaat gttgttccat aacgaaccag
      121 ccctgttcct ttaaactctg catcaatggc ataatgtata ctcaccagac tgagtttgct
      181 cctgcatcat tatttcgata cgttaatgca ttgttcattg catccggcat tccccatcga
      241 atatttactt tgttatatca gtcagcccat aaccaggtaa atttttaagg cttactgact
      301 gaatattcaa aatgtgttac atgttatcaa acctttaaag aataacctgc tcttcttcat
      361 tggccggatt tagctcaatc ttgtcagcaa aactaatata aagccgttca taaagagaag
      421 gatgtagctt cgttatttat cttttacatg taacagtgcc aggggatatt ccgcacatct
      481 ctgtggctat catccccacc acgtctccag tggcagcacc tgcggcactg gcacgggcgt
      541 tatttccctg taccaccgcc agtgcagcat tcaccacggc atgagctgcc acatttgcag
      601 tcttattcac cacacctgta acctgtctct tatacacaaa tccccacccg gggatttgtg
      661 atgtctgtca gtctgcttta gggggactct ttccatccgg tagtttcctt cctccccggc
      721 agcgattttt tgaacttttt cttcacccgt tgatcttctc ttatatttca ttttcttcgg
      781 gtctgttatg gtactttctg attgttattc actggctaac gagcagtctg gacatgcccg
      841 tctcggtgat ccgcgtcgta cacgacgcct tgtgtcactg acctcttctc tcgctcagca
      901 tgcaggtctt tctattgtga aatcatccca cttcactgct caggttgaag gtgcgtaccg
      961 cctgatacgt aacccgtcgg tgtccccgta agccattgcc gaagccggat ttactgccac
     1021 tgcaagggca tgtgaggcac atccgctgct cctcgcgctg gaagatacca ccaccattaa
     1081 cttcggtcac agtacggcat ttgatgacct ggggaatacc accactaacc cgaagacgca
     1141 cgggctgctg gctcactctg tcctgatgta cgcccccgac agcgccctgc cagtcgatct
     1201 tatcgaacag cagtgctgga gtcgtgccac ggacacctac ggtgttaagc accagcgaaa
     1261 agaacgtccc tgtgaagaaa aagaaagtta ccgctggcag caggcttcgg agcgcatggc
     1321 cgaacggctg agggagatac agaaacaggt aatcactgtc tgtgaccggg aagcggatat
     1381 ctggcattac ctgcattata aagtcagccg cgggaagcca cacgctgaat gtgatgcaaa
     1441 aaggcgggcg ggcagcccgc caggcccgga tgctcatcag ttacagcgaa gtcagcataa
     1501 aaaatcccga caacagcggc caggcgctct cgctcacgta tgtctgttgc ggggagcagg
     1561 agaggacgga gcctgctggt atccgctgac atcagaaaaa gtggcgagtg ccgccgatgc
     1621 gcgacgtatc gtcagccatt acgagcaacg ctggctgata gaggaatacc acaaagcgtg
     1681 gaaaagtggt ggtacatgtg tggaatcgct gcgaatgcag acccgggata accttgagcg
     1741 catggtggtt atcgcggtgc gggtgctggg cctgcggcag ggaggtatca gtgaagaaac
     1801 acaaaatgac agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa
     1861 gctggaagga aagcagcttc cggcgcaaac accgaccctg aaatgggcct gtttgaaact
     1921 ggctaaactg gggcgatggc atgacagcaa acgcacaagc agccccggtt gggtagttat
     1981 gtgggatggc tggttcagac atcaggatat ggctgagggt tacctggtga tgaagtctct
     2041 tgatcaggag atctgatcaa gagacaggtc aaagatgggg gacaacgtct ggtgctgtat
     2101 aacagcacag gcctcacact cttgacgccc gtggaatata ttagagaaca aaaatttcaa
     2161 tatgtggtgg acgcatacag aggattgaca gcattcccat actaattgtt aagattcaaa
     2221 ttgaagtata tctatctgtc attttgcaaa tgatatgcgg tagctttacc taactatcat
     2281 gaacatttcg aaaaatcatc cacaaagccc cgaagaataa agcactatcg cccgataaag
     2341 acctgtaagc tcagtggcat ggatactatt gagctcagaa tggcgagctg taccgttatt
     2401 tcattaccat gctcgacgaa cacagcaact actctctggc cgtactatcg cttaaaagtg
     2461 atatcatcag gcatttcttc tgctgtgtag cccagttgtt ccttgttggt atcaaccaga
     2521 tcatcactga tgatgggcaa agagttctcg ggaaaattcg acaaaacgct acaggaagcc
     2581 accgtcgggt tcgtatggac ttatctctat acgccgaaaa taaatgccat aagtgagcat
     2641 tttaaccgaa cactgaggga acaatttatt gagtttaatg aagaattgct ctttgaatag
     2701 ctgaatatgt tcaatctgaa actggctgag tatctggtac tgtacaacag taaaattctt
     2761 cataaatcgt ttaaactaca aaaatcagta gaatatatta tttccactgt caggcggaaa
     2821 atttatagaa aggatacaaa catatgccaa t
//
LOCUS       DAZGXS010000271         2840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGXS010000271 DAZGXS010000000
VERSION     DAZGXS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(140..784)
                     /locus_tag="ACHKG5_004855"
     CDS             complement(140..784)
                     /locus_tag="ACHKG5_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0870774.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            882..1109
                     /locus_tag="ACHKG5_004856"
     CDS             882..1109
                     /locus_tag="ACHKG5_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0870775.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            1106..1531
                     /locus_tag="ACHKG5_004857"
     CDS             1106..1531
                     /locus_tag="ACHKG5_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0870776.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            1600..2637
                     /locus_tag="ACHKG5_004858"
     CDS             1600..2637
                     /locus_tag="ACHKG5_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0870777.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAAPGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAV
                     EALDCSDWRDD"
     gene            2549..>2840
                     /locus_tag="ACHKG5_004859"
     CDS             2549..>2840
                     /locus_tag="ACHKG5_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0870778.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP"
BASE COUNT          746 a          618 c          786 g          690 t
ORIGIN      
        1 gtattgcccg acgttctgtg gggcgaggta gggtggtatg taataaatct aaaagtattt
       61 agtttttcag tcaaggtaaa tctagattaa tttaaaaaaa aggtcggcat tgccgacctc
      121 aagtaaagga cgtggcgggt tataagttga actgtacacc ttttgctacg gcaacaattt
      181 tacactctgg tgtaagtaag gatgactgat agcgtggatt gagagggctt aaatacacaa
      241 gcttcccatc aataactaat ttctttatag tcatagatgg ttcattttta agcggatccg
      301 gaactattac agcgacgatg ctgccatttt tataactttc tcctggcctt aggatcacag
      361 tagcaccaac tgggatactc ggtgatcctg atgggttatg cattgtgtca tcaggcattg
      421 aaacggcaaa atcaccttcc tcgacatcaa agaatgtggt gattctatcg acatttccca
      481 ttgttttctc tccttttaag attaggaaag aaatcgcctc accccacgaa aggtagggga
      541 tcttggtgcc tgaggtactt tgtacaaaag ataattcagg agacgatact ccatatagga
      601 gataggactc agtagttcct aatgcctggg ctaacttact tagagctttg ctacctggtt
      661 cgtttagatc tttctcccag taccctatag taaccccagt cacgcctgaa agcttaccca
      721 gttctacttg ggtcagcccc ttatcttttc taagtctctt aagcctgaag ccaaggcttt
      781 ccatcatttt ctcctgcaaa ttgaatataa attattttag attgcattga cctaaaaaaa
      841 attatcttgt aatctaaaaa tgcttagatt taggaggtaa aatgcgagtt gatgaacttg
      901 tccagttttt tggttctgtt cagagggttg ccgattttta tgggataacc cgcgaagcta
      961 tttacatgtg gcgtaagcgt cctggtgaaa tagttccgaa ggggagggct gcggaagctg
     1021 ctgcatactc caaggggaaa ttatctttga acccagaact ttacaaaaag aaggatacca
     1081 ctcagggcga aggaaagggg gattcatgaa aatcaaacat gagcacatcc gcatggcgat
     1141 caatgcctgg gcctatccgg acggtgagaa agttcctgca gctgaaatag cccggactta
     1201 tttcgagctg gggatgacgt tcccggaact gtacgacgac agccatccgg aagccctggc
     1261 tcgtaatacc cagaaaattt tccgttggct ggataaagac acccctgatg ctgttgaaaa
     1321 aattcaggct ctgttaccgg cgatcgaaaa ggcaatgccg cctctgctgg tggcccgtat
     1381 gcgcagtcac agctctgaat attaccgtga gatcgtcgaa cggagggatc ggctggtgaa
     1441 agatgtggat gattttgtcg cagcggcgat cgcctggggc accctgacta acagtggtgg
     1501 tcagcctggt aatgctgttg tcgtgcattg accaacaata ttcatgccgg atttcttccg
     1561 gatgttcgag ggtaaagttc ggtatcagat gaggtgagta tggctaatgc ctggctcaga
     1621 ttgtggcatg acatgccaaa tgatcccaaa tggcgaacca ttgccagggt ctcaggacag
     1681 ccaatcgcaa cagtgatggc ggtgtatatc caccttctgg tgagtgcgtc acgaaatgtc
     1741 acgacatgtc acggcgtgtc actacgtggt cacattgatg tcacgacgga agatttagca
     1801 agtgcgcttg atgtgacgga agacgtgatt gattcaattt tgcatgcaat gcaggggcga
     1861 gttctggatg gtgatcttat ttccggatgg gaaaaacgcc aggtgatgaa ggaggataac
     1921 ggtaatgttt cgcaaaccgc aaaatccccg gcagagcgca agagagcgca gcgggagcgg
     1981 gaaagactgc ggaagcagaa cactgattgt cacgatgagt cacgacgcgt cacgcatatg
     2041 tcacgacaaa tcacgacaga tacagataca gataaagaat taaaccccac acataacgcg
     2101 cgcatgcgcg agagtgctac gaccggtgag ttgaatgacg cgtcgttgca gacagccgaa
     2161 cctgaatacc tggacggcct gagcgaaccg atcgggaaat ttccgatgac tggagtctgg
     2221 caaccgtcgc cggattttcg acagcgggca gcagtgtggg gtatggctct gcctgagcct
     2281 gagtttacac ctgctgagct tgccgcattc cgggattact ggatggcgga ggggaaggtt
     2341 ttcacgcagg ttcagtggga gcagaaattt gcccgtcacg tgcagcacgt cagggcacag
     2401 gtaaaaccag tcagcaaggg ggtaagccat gcagcaccag gtggcaccgc atcacgggca
     2461 gttcaggaaa ttcgggcagc acgtgaacag tgggaacgtg aaaacggatt tatcagcaac
     2521 ggaaacggcc tggaagctgt gggaacttat gggggaggtg tattcgaacc gctggaccca
     2581 gaagaacggg gctgcgccgt cgaagctctg gattgctcag attggcgcga tgactgaaca
     2641 gcaaatccgg caggtctgcc gtcagtgcat ggaccgctgc cgggcgggtg aaacgtggcc
     2701 tccggatctg gctgagtttg tggcgttgat ttcggagagc ggggcaaatc catttggcct
     2761 gacggtggat gccgtgatgg aggagtaccg tcgctggagg aatgaatcct ggcggtacga
     2821 cgggagtgat aaatacccgt
//
LOCUS       DAZGXS010000272         2767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGXS010000272 DAZGXS010000000
VERSION     DAZGXS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            186..1352
                     /gene="nhaA"
                     /locus_tag="ACHKG5_004860"
     CDS             186..1352
                     /gene="nhaA"
                     /locus_tag="ACHKG5_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0870779.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1418..2317
                     /gene="nhaR"
                     /locus_tag="ACHKG5_004861"
     CDS             1418..2317
                     /gene="nhaR"
                     /locus_tag="ACHKG5_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0870780.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2355..>2767)
                     /locus_tag="ACHKG5_004862"
     CDS             complement(2355..>2767)
                     /locus_tag="ACHKG5_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP0870781.1"
                     /translation="EDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDV
                     EYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHE
                     GSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          644 a          607 c          710 g          806 t
ORIGIN      
        1 tatcagggct tagggtcacc cgtcagcgct tacagccgtc aaaaacgcat ctcaccgctg
       61 atggcgcaaa ttcttctata gatcgtaaaa aacgaattat tcctacacta taatctgatt
      121 ttaacgatga ttcgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat
      181 aaaaagtgaa acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta
      241 tcattgccgc tatcctggcg atgatgatgg ccaacagcgg cgcaaccagt ggatggtatc
      301 acgactttct ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca
      361 tgctgttatg gataaatgac gcgctgatgg cggtattttt cctgttagtc ggtctggaag
      421 ttaaacgtga actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccagtta
      481 tcgctgctat tggtgggatg attgtgccgg cattactcta tctggccttt aactatgccg
      541 atccgattac ccgcgaaggg tgggcgatcc cggcggctac tgacattgcc tttgcacttg
      601 gtgtgctggc gctattggga agtcgtgttc cgttagcgct gaagatcttt ttgatggctc
      661 tggctattat cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact
      721 tatcgatggc ctctcttggc gtcgcggctg tagcaattgc tgtactcgcg gtattgaatc
      781 tgtgtggtgt acgccgcacg ggcgtctata ttctggttgg cgtagtgttg tggactgcag
      841 tgttgaaatc gggggttcac gcaaccctgg cgggggtaat tgtcggcttc tttattcctt
      901 tgaaagagaa gcatgggcgt tctccagcga agcgactgga gcatgtgttg cacccgtggg
      961 tggcgtatct gattttacca ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggcg
     1021 tcacgctgga tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg
     1081 gcaaaccgct ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc
     1141 tgcctgaggg aacgacttat cagcaaatta tggcggtggg gatcctgtgc ggtatcggtt
     1201 ttactatgtc tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta
     1261 actgggcgaa actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct
     1321 ggttacgcgt tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac
     1381 ggctcccttt tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca
     1441 cttgtattac ttctggcatg tttataaaga aggttctgtg gttggcgcag cggaggcgct
     1501 ttatttaaca ccacaaacca ttaccggaca gattcgggcg ctggaagagc gcctgcaagg
     1561 caaattattt aagcgcaagg gacgtggtct cgagcccagc gagctggggg agctggtcta
     1621 tcgctatgcc gataaaatgt tcaccttgag ccaggaaatg ctggatatcg tgaactatcg
     1681 caaagaatcc aatttattgt ttgacgttgg cgtggctgat gtactttcca aacgtctggt
     1741 cagtagcgtg ctgaatgctg cagtagtaga aggcgagccg attcatcttc gctgcttcga
     1801 atccacccac gaaatgctgc tggagcaatt aagccagcat aaactggata tgatcatttc
     1861 cgactgtccg atagattcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg
     1921 tggcgtgagt ttctggtgta caaatccacc gccggaaaaa ccgtttccag cttgtcttga
     1981 agaacggcgg cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tacttaactg
     2041 gtttaactcc cagggattaa acgtagaaat ccttggcgag tttgatgatg ccgctttgat
     2101 gaaagctttt ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga
     2161 cttttatgcc gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta
     2221 ccatgctatt tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac
     2281 ggattattct gcgcttttta gtccagcggt gcgttaatcg gcagctcccc caaagttaag
     2341 gtgggggaga tagattagtt gtacattacc acgattttga ctcggctcat tatttgcccg
     2401 cttgagacat tgtttccata tgtacgcggg cgaataaata gaggaatctg attacttcct
     2461 tcatggggat gctgaaaaga gtagtaattg cttttaccat actggatttc attcgtacca
     2521 tcattggaga gttgaaagcc tatttgaccc gcaccgtcct cactgttttc taactcattt
     2581 tttaatatgc tggttcctga ctcctgtatc gtcactggtt caaagtaata ttcaacgtca
     2641 ctgggttcaa aaactaattc accttgctcg ccctggatgc agtcaaatct tatatgatga
     2701 aactgataat tttcattatt gatacgagac agtacaaatc gtccagtgtc gatggattga
     2761 tcttcaa
//
LOCUS       DAZGXS010000273         2757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGXS010000273 DAZGXS010000000
VERSION     DAZGXS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..747
                     /gene="paa"
                     /locus_tag="ACHKG5_004863"
     CDS             <1..747
                     /gene="paa"
                     /locus_tag="ACHKG5_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP0870782.1"
                     /translation="MAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTGIIVN
                     VHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSILLVKK
                     GNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRKNIIL
                     YAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQDA
                     NDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1083..1238
                     /gene="hokD"
                     /locus_tag="ACHKG5_004864"
     CDS             1083..1238
                     /gene="hokD"
                     /locus_tag="ACHKG5_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0870783.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1459..1716
                     /locus_tag="ACHKG5_004865"
     CDS             1459..1716
                     /locus_tag="ACHKG5_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870784.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1786..2064
                     /locus_tag="ACHKG5_004866"
     CDS             1786..2064
                     /locus_tag="ACHKG5_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870785.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2066..>2757
                     /locus_tag="ACHKG5_004867"
     CDS             2066..>2757
                     /locus_tag="ACHKG5_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0870786.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          718 a          650 c          731 g          658 t
ORIGIN      
        1 atggcaggtt ttttaatatt cctgtcttct gctgcttatg ctgatatcaa tctgtatggt
       61 cctggtggcc cgcatacagc cttgcttgat gcagccaaac tttatgccga aaaaacaggt
      121 attatagtga acgttcatta cggcccacag aacaaatgga atgaagatgc caaaaaaaat
      181 gcagatatct tgtttggcgc atcagaacaa tctgctctgg ctatcattcg ggaccataaa
      241 gacagcttca gtgaaaaaga tattcagcct ctttatctgc gaaaaagtat tttactggta
      301 aagaaaggta atcctaaaaa tatccggagt attgacgacc tgaccagacc tgggattggc
      361 gtaattgtta atgatggtgg tggtaccagt aatacatcag gcactggcgt ctgggaagat
      421 attgccggac gtaaagggaa tatagaaact gtcgccgcaa tccgaaaaaa tattatttta
      481 tatgcgccca atagcggaac tgcacgtaag gctcttgaga atcagcctgg agcagatgtc
      541 tggataacct gggctgactg ggcagccagt aatccagaaa ttggtgatgt cgtggaaata
      601 gcgccagact acgtgatatg gcgtgatatg aacattacag tacgtcagga tgcaaatgat
      661 gaaacccgtc gatttgcaga atggctacaa accgatgaag cggcgcctgc attcaaaaaa
      721 tatggctgga ccaggaaagg cacttgacat cctcgtcctt caggacgtgg attctttttc
      781 cggatgccgc gccagcggca tgtaggggca gctcacaaaa cggaaaaaat tgtacgctaa
      841 gcctcgccag gtgaactgaa ttcattccga tatgggaatt cccatatcgg gcgaaaacgg
      901 tttgctgtaa cggcagagtt aagtaggatt gctgcgggtg cttgaggcta tctgcctcgg
      961 gcatgaacac caacggcaga tagataaaag ccccacccga ctataaatcg aagtgaggcc
     1021 cctatatgct cgtcacatat agattgcctc ttacggaccg aaaggtcaag gagaagcagg
     1081 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta atcgtcatag
     1141 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     1201 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     1261 cctcgccacc tctgatgtgt caggcatcct caacgcaccc gcacttaacc cgcttcggcg
     1321 ggtttttgtt tttattttca acgcgtttga agttccggac ggcgccggaa tagaatcaaa
     1381 aatacttaag tagcgcgcag ggagaagagg gatggacccc gaacagggga gtgctattta
     1441 tctggaagga ttctgttgat gagaatcgaa gaattacgtg aaatttttag tgaagatggc
     1501 ctctatactg tgcgcgttga gaagggcgct attgtcagcc actgccgtat taaatgttta
     1561 cagtctcaac aaaggaagag tggagctgcg ttaattcatt ttgtggatgg gcttgtgacg
     1621 gatggtttta ttttgcgtgc aaatgaattt gtcacatcgt tgccgtctct gaaagaagct
     1681 gggattaagg ctggtttttc tgcttttgaa gattagtgaa ttcatctaca attcagcgca
     1741 gggctgaacc cctgttgagt aacactgtgc caccggagaa agccgatggc gcaaaattcc
     1801 agactacaca attctgataa ttcagccgtc tttgccagca ggcacgggcg gcgttctcat
     1861 gcattcaaat ctgactggtt ccggcacgcc ccatgcactg aagaacaggc cgaatggctg
     1921 attcagaact accgcagacg tgggtatgag tttaggaaag ccctcagcct cgattatcgt
     1981 cactggataa tctacgtcag gctgccgtac tccgagcgcc caccgcgtcc gtcccgcaca
     2041 ttccagcaac gcatctggag gtaacgtgcg ggtattactt cgacctgttc cggtaccgga
     2101 acttggggtg gtggtcctta aaccaggccg tgaatccatg caggtattcc ataaccctcg
     2161 agtgcttgtg gagccggagc cgaaaagcat gcgcggtctg ccgtccggca tcgtccctgc
     2221 cgttcgccag ccgctggcgg aggataaatc attactgccg tttttcagca atgagcgtgt
     2281 gattcgtgct gttggtggcg ctggtgcact gtctgactgg ctgttgcgtc atgtcaaatc
     2341 ctgccagtgg cctcatggtg actatcatca cagtgaaacc gttattcacc gttatggtac
     2401 cggcgcaatg gtgttgtgct ggcactgcga caaccagctg cgtgaccaga catccgaatc
     2461 actcgagcaa cttgctcatc aaaacctgtc agcatggatg attgacgtca tcggtcacgc
     2521 aataagcggt acgcaggagc gtgaattatc tctggctgaa ttatcctggt gggcggtccg
     2581 caatcaggtg gcggacgcgc taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc
     2641 ggaaaaaatc cgctcaatgt accgtgaaag cgacatcgta ccgggagagc agaccgccac
     2701 catcatactg aagcagcgca caaaaaatct tgcgccgctg cctcacgccc accagca
//
LOCUS       DAZGXS010000274         2742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGXS010000274 DAZGXS010000000
VERSION     DAZGXS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>2742
                     /gene="ehxA"
                     /locus_tag="ACHKG5_004868"
     CDS             <1..>2742
                     /gene="ehxA"
                     /locus_tag="ACHKG5_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP0870787.1"
                     /translation="SAFNTASSSVRSAGKKLILLIPDNYEAQGVGINELVKAADELGI
                     EIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIGGTAEN
                     VGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKSSIELINQLV
                     DTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGPLGDGLDVVSGILS
                     AVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTTAASAG
                     LITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKETGAIDA
                     ALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFEHVAEK
                     FAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQQHWDE
                     KIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLSTGNVS
                     SVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDYSKLIQ
                     FVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYDTVSYNKTDVGKLTIDATG
                     ASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVIDNL
                     HSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQGDD
                     QLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGIDLLDG
                     GEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVGNDLIM
                     NKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKIPHNNN
                     "
BASE COUNT          881 a          423 c          676 g          762 t
ORIGIN      
        1 tcagcattta atacagcatc atcaagcgta cgttccgctg gaaaaaaact catattatta
       61 atacctgata attatgaagc tcagggcgtg ggtattaatg agttggtcaa agctgctgat
      121 gagcttggaa tagaaataca ccgtactgaa cgagatgata cagcgattgc aaaccagttt
      181 tttggtgcag cagaaaaagt tgtaggatta actgaacgtg gtgttgcaat attcgcacca
      241 caacttgaca aacttctgca gaagtatcag aaagttggga gtaaaatagg aggaaccgct
      301 gaaaatgtag gtaataatct gggaaaagcc ggaacagttc tctcagcact acagaatttt
      361 acggggattg ctttatcagg catggctctt gatgaattgc tgagaaaaca acgggaagga
      421 gaggatataa gtcagaatga tattgccaaa agtagtattg aacttattaa tcagcttgta
      481 gatacagtat caagtataaa cagtaccgtt gattcatttt ctgagcagct taaccagctt
      541 ggctcatttt tatccagtaa acctcgatta agttctgttg gtgggaaatt acaaaattta
      601 ccagacctgg gccccctggg ggatgggctg gatgttgtct ccggaattct ttctgctgta
      661 tcagcaagct ttattctggg aaacagtgac gcacatacag gaacaaaagc tgcagcgggt
      721 atcgaactga caactcaggt tcttggaaat gttggtaaag ctgtttcgca atatattctg
      781 gctcagagaa tggcacaggg gttatcgaca acagctgcaa gtgcgggtct gatcacatcg
      841 gctgttatgc tggctatcag tcctctttct ttcctggctg ctgcagataa atttgagcga
      901 gctaagcagc ttgaatcata ttctgaacga tttaaaaaat tgaattatga aggggatgct
      961 ttactcgcag cctttcataa agaaaccgga gctatagatg cagccctgac aacaataaat
     1021 actgtcctga gttctgtatc tgcgggagtt agtgcagcct ccagtgcatc cctcataggg
     1081 gccccgataa gcatgctggt gagtgcatta accggtacga tatctggcat tctggaagca
     1141 tcaaaacagg ctatgtttga gcacgttgca gagaaattcg ctgctcggat caatgaatgg
     1201 gaaaaggagc atggcaaaaa ttattttgag aatggatatg acgcaagaca tgctgcgttt
     1261 ttagaagact ctctgtcttt gcttgctgat ttttctcgtc agcatgcagt agaaagagca
     1321 gtcgcaataa cccagcaaca ttgggatgag aagatcggtg aacttgcagg cataacccgt
     1381 aatgctgatc gcagtcagag tggtaaggca tatattaatt atctggaaaa tggagggctt
     1441 ttagaggctc aaccgaagga gtttacacaa caagtgtttg atcctcaaaa agggaccata
     1501 gacctttcaa caggtaatgt atcaagtgtt ttgacattta taacaccaac atttacccca
     1561 ggagaagaag ttagagaaag aaaacagagt ggtaaatatg aatatatgac atctcttatt
     1621 gtaaatggta aggatacatg gtctgtaaaa ggcataaaaa atcataaagg tgtatatgat
     1681 tattcaaaat tgattcagtt tgttgaaaag aataacaaac actatcaggc gagaataatt
     1741 tctgagctcg gagataaaga cgatgtggtt tattctggag caggctcatc agaagtattt
     1801 gctggtgaag gttatgatac cgtatcttat aataagacgg atgttggtaa actaacaatt
     1861 gatgcaacag gagcatcaaa acctggtgag tatatagttt caaaaaatat gtatggtgac
     1921 gtgaaggtat tgcaggaagt cgttaaggaa caggaggtgt cagtagggaa gcgaacagag
     1981 aaaatacaat atcgtgattt tgaattcaga accggtggaa ttccttatga tgtaatagat
     2041 aatcttcatt ctgttgaaga gctcattggc ggaaaacatg atgatgaatt caaaggcggt
     2101 aagtttaatg atatattcca tggcgcagat gggaacgatt atatcgaagg taattatggt
     2161 aatgatcgac tatacggcga tgatggggat gattatatat ccggaggaca gggagacgac
     2221 cagttatttg gtggtagtgg aaacgataaa ttgagtggag gggatggtaa taattatctg
     2281 acaggaggaa gcggtaatga tgagcttcag gcacacggag cttataatat tctgtcaggt
     2341 ggtactggtg atgataaact ttatggtggt ggtggtattg atcttctgga tggaggggaa
     2401 ggtaatgact atctgaatgg tggttttggt aatgatattt atgtttatgg gcaaaactat
     2461 ggtcatcata caattgcaga tgaaggaggt aaaggagatc gtttgcactt atctgatatt
     2521 agctttgatg atatcgcatt taagagagtt ggaaatgatc ttatcatgaa taaagccatt
     2581 aatggtgtac tttcatttaa tgagtcaaat gatgtcaatg ggataacatt taaaaactgg
     2641 tttgcgaaag atgcctcagg agcagataat catcttgttg aggttataac agataaagat
     2701 ggtcgagaga taaaagttga taagatacct cataataata at
//
LOCUS       DAZGXS010000275         2676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGXS010000275 DAZGXS010000000
VERSION     DAZGXS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..358
                     /locus_tag="ACHKG5_004869"
     CDS             <1..358
                     /locus_tag="ACHKG5_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870788.1"
                     /translation="VSILVNDIFMKNTEMINWYFPRLLKSYEDEKIYFDKLGYNFNNK
                     ESNEEIMKNQPKDVIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPPENL
                     RIAIEKFGWKKKTITA"
     gene            complement(415..1419)
                     /gene="argF"
                     /locus_tag="ACHKG5_004870"
     CDS             complement(415..1419)
                     /gene="argF"
                     /locus_tag="ACHKG5_004870"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0870789.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1581..1997
                     /gene="rraB"
                     /locus_tag="ACHKG5_004871"
     CDS             1581..1997
                     /gene="rraB"
                     /locus_tag="ACHKG5_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0870790.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(2043..2546)
                     /locus_tag="ACHKG5_004872"
     CDS             complement(2043..2546)
                     /locus_tag="ACHKG5_004872"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0870791.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          736 a          616 c          610 g          714 t
ORIGIN      
        1 tgtcagtatt ttagttaatg atatttttat gaaaaacact gagatgatta attggtactt
       61 ccctcggtta cttaagagct atgaggatga aaagatttat tttgataagt tagggtataa
      121 ttttaataat aaagagtcta atgaagagat tatgaaaaat caaccaaaag atgttattga
      181 agaaaaactt aataatgaat taaaacttag gtttagaatg atgcaaacta tcttgaaatc
      241 ggaggttaat gtatcgccat ttattgacca acagcgttta aatacactaa atcctccgga
      301 aaatttacgt atagcaatag aaaaatttgg ctggaagaaa aaaactatca ctgcataaaa
      361 taatttgacg ccgggatgtt tctgtatttc ccggcatctt tatagcgata tcaattattt
      421 actgagtgtc gcgaccatca ccgctttgat ggtgtgcatg cggttttccg cctgatcaaa
      481 aacaatgctg gcggcagatt cgaagacctc atcagtcact tccattccgc catgtaggcc
      541 aaactcttcc gccattttct tgccaagcgt cgtttggtca tcatggaacg cgggcaggca
      601 gtggaggaat ttgacctccg ggttaccggt caactgcatc atcttgctgt tcacctgata
      661 atcacgcagt aatgcaatgc gttcagccca tttttctttt gcttcaccca tcgacaccca
      721 tacatcggta tagataaagt cagcaccttc aacgccttta gctacatctt ccgtcagcgt
      781 aatattcccg ccattttgtt gtgccagggc gcggcattcc gtaaccagcg cagcttccgg
      841 ccagcatgcc tgtggcgcga ccagacgcaa atccagaccg gtaagcgccg cagcttcgag
      901 catcgaattg cccatgttgt tacgtgcgtc acctgcatag accagcgtca tttcgttgaa
      961 tggtttaccg ggtaaatgct cctgcatggt gaggagatcc gccagcaact gcgtggggtg
     1021 aaactcattg gtcaggccgt tccataccgg cacgccagcg tattccgcca gtgtttcgac
     1081 aatctcctga ccatagccgc gatactgaat accgtcatac atgcgaccca gcacgcgggc
     1141 agtgtcttta atcgactctt tatgaccaat ctgactgccg cttgggccga gataagtaac
     1201 gcgagcaccc tggtcatatg cggcaacttc gaaagagcat cgggtacggg tcgagtcttt
     1261 ttcgaagatg agcgcgatgt ttttaccagt gagtttggct tcttctttgc cgcttttctt
     1321 atcggctttc agcttcgcgg ctaactgcag caggctgttg agttcagctg gcgtgaaatc
     1381 gagtaatttc aggaaatgct tatgataaaa cccggacata gatccctcct gtggctaacg
     1441 tctcaatgaa ttaaaattca atttatatgg atgattattc atttgcaagt ctaaagcata
     1501 aatctttgtc acaaaggtgg aggcaatgcc agtggtgtgt gacaatatga gtatcggcag
     1561 gacattaaga ggaatgagcc atggcaaacc cggaacaact ggaagaacag cgtgaagaaa
     1621 cacgtttgat tattgaagaa ttactggaag atggcagcga tccggacgcg ctgtacacca
     1681 tcgaacacca tctttccgca gacgatctgg aaaccctgga aaaagcagca gttgaagcgt
     1741 ttaaactcgg ttacgaagtg accgatccag aagagctgga agttgaagat ggtgatatcg
     1801 tgatttgctg cgacatcctc agcgagtgcg cgttgaatgc cgatctgatc gatgcccagg
     1861 ttgaacaact gatgacgctg gcggagaaat ttgacgttga gtacgacggt tggggcactt
     1921 actttgaaga tcccaacggc gaagacggcg acgatgaaga ttttgtcgat gaagacgatg
     1981 acggggttcg ccactaatta attgattaaa atgcccgata gcacacacta gcgggcattt
     2041 ttttagagtt cgcgtagcat ccgcacttca caatcaacat ggcccgtgca gccaagtgca
     2101 tagtcgatat gttgaaaacc taaatgttca tacaaaccaa tcgcttcttt caaaaaagcg
     2161 gtcgtttcca gatagcagcg tttgaagccc atttctcttg cctcctccat cgcctttaag
     2221 gccagttttt ttgccagccc tttgccgcga atagccggaa gaaaatacat cttttgcagt
     2281 tcgcaaatgt ccgattcact acctgctaat ggcgcaatcc caccgccgcc gaccacttca
     2341 ccctcgtact caaccaccca atatgcatga cccggctgac tatacacttg atacagctcg
     2401 tcaagattcg gatcggcgac ggtgtagcct ttatcagcgg taagaccgta ttcggcggat
     2461 acctgacgaa tgacgcgggc gatagcagga ttatcctgca gcgtcaggcg acgcattaca
     2521 ggtgattgcg gcgcaatgtt attcatagtt taacccaata aaaataaagt aattatacat
     2581 ttgtttaata ccactctctt cttacgtgcg caagcgtctt ataaagcaat aaagtttttg
     2641 ccagcctatt actgataaga gtattgtaac gtggca
//
LOCUS       DAZGXS010000276         2669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGXS010000276 DAZGXS010000000
VERSION     DAZGXS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..1161)
                     /locus_tag="ACHKG5_004873"
                     /pseudo
     CDS             complement(1..1161)
                     /locus_tag="ACHKG5_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(1402..2448)
                     /locus_tag="ACHKG5_004874"
     CDS             complement(1402..2448)
                     /locus_tag="ACHKG5_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP0870792.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT          722 a          668 c          557 g          722 t
ORIGIN      
        1 aagtaaatag cgccagcctt ctctccggaa tcatcaatgg tttgactttc agtatcatga
       61 agtttaacaa caaatcaatt ctgtttctga atccaatccg cctgtcagaa aggtaatgaa
      121 tataagccat caggtcggtt gttttttgtg aggaaaatcg gtatttgtga gaaaaaagct
      181 cctccggtcg aagattctga ctctgccatt gctgcaggta tttatactca atattcagct
      241 cattaatttc tgcttttaac gaagcgacct ggttaatggc attcaggtgt tttttcactt
      301 gttgaagtac atcatcaatc gcatttgcag acggtgagtc aggtaagact tcctcagaac
      361 gcgaaatcga cgttgaaaaa aactgttgcc tgttctccgt actccccagt cgtgccatca
      421 catatcccag ttgctgtttg tccatctttt cataaacatt actgacagct gagttattat
      481 tggacaaaat tgccactgtt ttattctgaa ttaatatatt agcaacaata ttgagtatag
      541 tctgtgtttt acctgttcca ggtggtcctt caataactga aatctgtgag gaaaatgccc
      601 gttcgacagc cagcaactga ctttcattaa gaccaaacgg aaaaatcaaa ccccatggcg
      661 aatcacgttt ttactctgtc ctgaacagta tgcatgcaag gcggtatccg gatgaggaac
      721 tattttcttt atctgcctaa ggatattctc atcgatattt ctgttattac cacgggcata
      781 aaaaatacgt tcattagcca ccctacacaa gtaattaaac acggcatgct ctgtaaatgc
      841 tgaacactgt tgaaagtcaa ggccggtcgt tttcatgatg tagggtcgtt caccctcgcg
      901 gtaataaaca gcagcatatt tatcaccata tataataacc agttcagccc gggatgttac
      961 agcgttccct ttcctttcga ggaaaacatt tcgcaaattt acagttggcg ttatctgaca
     1021 tacagaaagc ggacgagtat attttctccc gctgcgaaaa tgacaggtca gcataacctc
     1081 tctgtcgtca tttccagccc acagcgtcca gtcggtaatt ttatgagtct tattttcacc
     1141 gtcaacacaa atcgtgacca tattcttact cagaaattat catttaactt acggaaaatt
     1201 gttaaggcac tcccgatatt agctccggag gattttccac aactccggga ctgttaacat
     1261 atttctgcct gccatatata cattgaattt agtcataaca gtcaatacca gaattacagg
     1321 aatgcgatgc cgatacgctg gttattgaat actggtagcc cctgagcact aattcatcac
     1381 gatactctgt atttactgtt ttcactgctt tttacaacca ttcccccagg taaaatgctg
     1441 ctcctccggt gatggctgtg tcagaaactg ccatcccgga atagccacgc tgaccgccag
     1501 caatgccgca cagacaccag ccacaaacgg tttcagaaac gtgggcgggg atttctgaag
     1561 ggagggtgac tgctccgcct gcactttccc cttgcgatta tcaaggctca gtgcatccag
     1621 acgcaccgcg ccatttgccg caagcagccc ttcccggtta tccacgcttc ccggcagact
     1681 cagcgaaaaa tcccctgttc ccgtctgaat cagactgcct ttacgatttg acaggctttg
     1741 tgccgatatc tgcagtgtgt cggcatttat cgtaccgcca ttactgctga actgcccggc
     1801 agtccgggcg gaaagcgtat cggcactcag ttgcgcattc tgtgtagaca gatttccact
     1861 ggttgcattc agctcagtat gttgtccctg tatccggctg ttacctaaat ccagcccctg
     1921 ctcaatgtcc cgtaaatcca gcagagaacc ttcgtgtgcc gggaatgagg aatacaactg
     1981 aatatagtca tcactgtccg gtgtcagacg cacatgtcgc acaacaccgg gaataataac
     2041 cagccggagg ataccgcttt ttaaatcctg cgaaggtgcc agaacacggg tggtgacata
     2101 accatgatcg accagacggt tctgcaatgt actcatcagc agattaattc ctttcgcccc
     2161 caggcaatgc ccgaccgccc cgttggcgat tttttgtaaa ggcagccagt gtggtaacgc
     2221 atcagccccc ttcagttccg tctgtttaat ctgaaaacag ggcgtttcaa caggaaaatt
     2281 aatcttatgg gcaaaagagc caggtgcaga aaggcgaaca tcaggggctg aaggcgtcag
     2341 ttgttgctca agggcttcct gacgctgctg ctgaatgata aatgcttcat ctccgctacg
     2401 ccggacatcc gctgcctgaa gagataaagg cgataataaa gcagacatca gtgtcagtga
     2461 cagcaaggac ggacagcgcc ggaataagct ttgttcctca gtcctctcgt tcctgcaaca
     2521 taattttcca gtcaaacgaa aaacctttta ttcatgtcat accaacaatc accatgaacc
     2581 gccccgggaa tcctggagac taaactccct gagaaagagg taaacaggat gactaaaaat
     2641 acacgttttt cccccgaggt ccgtcaacg
//
LOCUS       DAZGXS010000277         2664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGXS010000277 DAZGXS010000000
VERSION     DAZGXS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(35..241)
                     /locus_tag="ACHKG5_004875"
     CDS             complement(35..241)
                     /locus_tag="ACHKG5_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0870793.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(341..1192)
                     /locus_tag="ACHKG5_004876"
     CDS             complement(341..1192)
                     /locus_tag="ACHKG5_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP0870794.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1619..>2664)
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004877"
     CDS             complement(1619..>2664)
                     /gene="ypjA"
                     /locus_tag="ACHKG5_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP0870795.1"
                     /translation="VKPNPDPNPNPNPNPKPDPKPDPKPDPKPDPTPEPTPTPVPEKR
                     ITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVWGATYNTRNNVTTDAG
                     AGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVGSYSLGGYAS
                     WEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIETGMRFTDGNWNL
                     TPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEIEPWLKA
                     AVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSHGAGVE
                     SPWNAVAGVNWSF"
BASE COUNT          694 a          621 c          613 g          736 t
ORIGIN      
        1 taatcctcat aaataagaag actgtcattt tatttcaggc aacatgcttt cttgaatttt
       61 cgatagttaa ttgaatccat tcgtctactt cactttcaat gaaagcaaca gagcgcgcac
      121 cgattttaac gggggaagga aaacgatttt ggttgatgag gtgatagatc caagctttac
      181 cgtatccagt tcgatgcaga acctctggta aacgaataag gcgggtgttg tgctgattca
      241 tagtcgtttc ctctgtgccg ttttgatgga aatgactata caaatgaatc agcaggaaaa
      301 agaccgaagc tgggaacgcc agagctaatg gaggttggtc ttagtcagaa tcagcgattt
      361 tcatcacgtc tgaaactctg atctttttca aatggctgtt cttgttgatg aacagattaa
      421 actggcgaat aaaaggtata ctcgccgcct tcaaagcaag aggtttgtct gtatccctga
      481 tttggtggtt caggcgagtg ttgatctcat catcgtcatc tgtatacaac aggcgagaaa
      541 ggcggtcttc tgatatacgt actttatata attttgacca taccagaatg tcgatcatgg
      601 gaaggagtct gtaattaatg attttcttaa tcgttccata accaaagcga atagcttccg
      661 ttacatctgg ttctatgcca cgcactttac gccattgagg aagtgcagcc tttagggatt
      721 cagcgatctc ttcatctgtc ccgtttgcta ggtcgatttc gaccatgaca gtatccctga
      781 acaattttgg caaaacttcc gatacagggc tatcgaggaa ttctctgtta acagaatact
      841 catcgcttcc atcccaaaaa aacagtgagt tccgtaatcc aactatgctg agttgagcca
      901 gtctttcggt tgtcgtcaaa aagaagtgcg ggggctggta aagagtatcc tctcttgtga
      961 gatagccgag atgcttttct gtgatgagaa agggctttcc agtgaagatc ctgtcgatgt
     1021 agagccttac ggcttctgct tcaaattctt catgatatgg tttgaagaac aatgtccttg
     1081 ccagcaattc atggtagagg tgtataagcg gaagctcttc gaatccgcga tatgtatcta
     1141 tatccagcca cttcttcacc tctctcgtat gttcaactga ccagtttttc ataacgatct
     1201 ccaacaggca ttagacttaa tgtatgtagc aaacgtaatc gacatgttaa aggaagacca
     1261 atgaaatgag ttcagatgcc atcaggaagt tgaaccaaaa ggtttgatat tttttgatac
     1321 agttcgctaa cggactattt tcaggggttt atgccgttac gatttatggt tcctttatag
     1381 aaccaaaggt aaagtcagaa acagcagtta tctagaaaat tcaatcaaat agatatctag
     1441 ttacagggac gccagccaat catgattgga cggtgtaagg acaacaccaa caaaaacagg
     1501 aagttagaag tctcagcagg acaccgacca gacggtgagg agacataaaa ggatacgcaa
     1561 aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc gttgatggtc
     1621 agaacgacca gttcacacca gctaccgcgt tccacgggga ttccacaccg gcaccatggc
     1681 tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta atacctgcct
     1741 ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcatta ttcaccttca
     1801 cccggttatc atcgacaaat tctttgcgca cagccgcctt cagccacggc tcaatttcca
     1861 taccgttccc cagacgcatg ttgtaactca gcgttgcgcc cagttcacga tatatactgc
     1921 gggtatcgac tgatttcgat tccatgccat tggataaatg atattcgggg ttatcagcgg
     1981 tgaaccccgt taacgatgca tacggcgtca ggttccagtt accatcggta aatcgcatcc
     2041 cggtttcaat gtgaccgccc agcccgttgc tgtggtaact gccattggcg gctccaccgc
     2101 tgctcatttt accggctacg ttactttcaa aacggttcag cttcacgaca ccgtccagat
     2161 agaaaccact ttcatgttcc caactggcat agccgcccag agaataactg cccacactgc
     2221 catgtcctcc gcgatcaaaa ccgatatgtg aatgggaata acccataaaa gcgcccagcg
     2281 tcgcaatccc ctcaggaata tcattagggc tgtcgatccc cactgtcatt ccggtcagcg
     2341 tctgctcaaa cccggccccc gcatcggtgg tgacattatt acgggtgtta tacgtcgccc
     2401 cccagacatt attgttgtgt ggactcgctt tcattatgtt caaccgctcg cgaatactgt
     2461 ttagctcagc atcaaatacc aacggtaatg ttgctgccat attgagtacg gctgcggtag
     2521 aaggcgtgat gcgtttctcc ggaacgggtg tcggcgttgg ctcgggagtc gggtctggtt
     2581 tcggatccgg ttttgggtct ggttttggat ccggcttcgg atttgggttg ggatttgggt
     2641 tgggatccgg gttgggtttg acat
//
LOCUS       DAZGXS010000278         2661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGXS010000278 DAZGXS010000000
VERSION     DAZGXS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHKG5_004878"
     CDS             complement(<1..206)
                     /locus_tag="ACHKG5_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0870796.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDA"
     gene            complement(190..513)
                     /locus_tag="ACHKG5_004879"
     CDS             complement(190..513)
                     /locus_tag="ACHKG5_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870797.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            638..1114
                     /gene="racR"
                     /locus_tag="ACHKG5_004880"
                     /gene_synonym="rarC"
     CDS             638..1114
                     /gene="racR"
                     /locus_tag="ACHKG5_004880"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFP0870798.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1430..1582
                     /locus_tag="ACHKG5_004881"
     CDS             1430..1582
                     /locus_tag="ACHKG5_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0870799.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1697..2212)
                     /locus_tag="ACHKG5_004882"
     CDS             complement(1697..2212)
                     /locus_tag="ACHKG5_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870800.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCEDQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
BASE COUNT          741 a          628 c          585 g          707 t
ORIGIN      
        1 gcatcagggg tgtctttatc cagccaacgg aaaattttct gggtattacg agccagggct
       61 tccggatggc tgtcgtcgta cagttccggg aacgtcatcc ccagttcgaa ataagtccgg
      121 gctatttcag ctgcaggaac tttctcacca tcaggatatg cccaggcatt catcgccatg
      181 cggatgtgct catgtttgat tttcatgaat catttgcctc ttgatgcttc gggtatgatc
      241 gttttcgtca tttggttgct tcatcgacat attctgcgaa taacatgacg agcgtcgtaa
      301 gtatgtccaa tcaacatcag gacgaagttc ttcacacagg acaccacctt ttgttgctcg
      361 ttcaatcgca ggacatctct cagcaggcaa ctgacgtaca cctttgatcc attgatttac
      421 gcttggagga gatacaccta aaagcctagc cattgctgat tgcccaccga caacagcaca
      481 agctcgtttg aatgaatagt tatctttttt catcgaatga actccaaaaa acacacaata
      541 atattaggct tagcctaatg caattgtcaa taggctatgc ctaacgcttc gagagtaggt
      601 attgcctaat gcgatgcaca taggagacta ttaagcaatg cttagtggta aagacttagg
      661 ccgagcgata gagcaggcca ttaacaaaaa aattgcatca ggagccgtca aatcaaaggc
      721 ggaaatcgca cgtcatttca aagtccaacc accatcaatc catgactgga ttaagaaagg
      781 ttcgataagt aaagacaaac ttccagaact atggcgtttc ttttctgatg tggttggtcc
      841 agagcattgg gggcttaacg aataccccat accaacccca tccacttcag atacaaaaag
      901 tgaactttta gacataaaca gcctttatca agccgcctct gatgaaaaaa gagcaattgt
      961 ggctttcctc ttatctggaa atgctacgga gcctagttgg gttgatcatg acgttcgtgc
     1021 ctacattgcc gcaatggaaa tgaaggtagc taactatctg aaaaatcaag aatcaaaacg
     1081 gaaaagccag aacatcacca agacaggaac ttaaacttat atggtccgac gggaaattcc
     1141 tagttcccgt tagttaactc ctactacctc tcccacaaac catcacctat taggtcgcac
     1201 ccaaatcatt aggcgtagcc tattgacaag caattaggca tttcctatag ttttcccata
     1261 ccaacccatc ccgtcccaca caatacaggg caatacctag agttacccgg cagtggtcag
     1321 ggattaagta gccagcccga ggcgtaagaa catgacggca gggttcaact ttaactatgc
     1381 agcaggtttt tgttccgcta ccccggcgtt aaggggatat gaggtcagca tggatactct
     1441 tgatcttggc aacaacgaat ctctggtgtg cggcgtgttt cccaaccagg acggcacgtt
     1501 caccgcgatg acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgtcgctg
     1561 gttagcaaga aactctgact aatgaaatct ggtaattaag gaatcctcca cggggggaga
     1621 gtggtgcaca cgcgccggac acaagcaagc atccggcatg ctctttaaca atctggatat
     1681 ccataacagt aaaaatctac agattgccgt tcagttttct ggccaactct tcaatagatg
     1741 gaggtgatac gtaatccgga tttttattca taagaaactg atcttcacag tacaggcacc
     1801 tgcttttatg aaaaaattca tattctctaa ccgggaatgg ctgaagtatc gatactatct
     1861 tttgtccaaa acattttgga caaagatgca tgattatgct gccgccgttc acggttacct
     1921 ccttcgagta cacaaaagtt cccgactcaa gttggctaag gatataacct tccgtctgag
     1981 cctcaaagtt ttcaaattct gcaattttag ctttgagaga agcatttatt tcttgataag
     2041 tgcccaccag ctcaacgaga gacacgcatt cgcgctgaat agatgcaagc tttgagttca
     2101 gttcaccaat agccgcattt acttcagctt gagtttttgc ctcgttcatt agttttgcaa
     2161 tctgggcggt ttcacgaata gccgtcattg ctgccgttaa ttcagcgatc acattcaata
     2221 ctcttattgt tgttggggat atccagatta acaagatcct tgttgttggg gaataacggg
     2281 gaccacctcg cctgacgtgg taaaaagcag gcacacaaca cgaaagcaca cggcgaggtt
     2341 gctggttcat agatagccta tcgttaaatt ttcgtcgacc gtgcgcttcc ggttgtggca
     2401 gtccgcgaaa tggcgcggcg gtaagtatgg ctggggtttc ctccattgct ccagaaaatg
     2461 caccgggttg tcaggttgac catacgctta agtgacaacc ccgctacaac gccctctgtt
     2521 atcaattttc tggtgacatt tggcggtatc agttttactc cgtgactgct ctgccgccct
     2581 ttttaaagtg aattttgtga tgcggtgaat gcggctaagc gcacgcggaa cagttaaaac
     2641 caaaaacagt gttatgggtg g
//
LOCUS       DAZGXS010000279         2571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGXS010000279 DAZGXS010000000
VERSION     DAZGXS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            404..547
                     /locus_tag="ACHKG5_004883"
     CDS             404..547
                     /locus_tag="ACHKG5_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0870801.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            560..769
                     /gene="cspE"
                     /locus_tag="ACHKG5_004884"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             560..769
                     /gene="cspE"
                     /locus_tag="ACHKG5_004884"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0870802.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            935..1744
                     /gene="rlmA"
                     /locus_tag="ACHKG5_004885"
     CDS             935..1744
                     /gene="rlmA"
                     /locus_tag="ACHKG5_004885"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0870803.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1741..2307)
                     /gene="mntP"
                     /locus_tag="ACHKG5_004886"
     CDS             complement(1741..2307)
                     /gene="mntP"
                     /locus_tag="ACHKG5_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0870804.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2304..2426)
                     /locus_tag="ACHKG5_004887"
     CDS             complement(2304..2426)
                     /locus_tag="ACHKG5_004887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870805.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          685 a          605 c          617 g          664 t
ORIGIN      
        1 tttttcttat tttaaatttc caattccttt gggattgact tctctttggg gtgattgaga
       61 gccgttaact gcctgtttta tgagaaaaag tgatataact ttttattcat tgcatagcag
      121 aaaatgtgat attgcacgca ctatgtaata acttctccca ctgacctgga acaactgaac
      181 ttattgaact atgttagaaa ctacgtcagt ttaagtatct gcggaactga caaggttaag
      241 cacaatgata tatcggcgcg tattccgttg cataagtgtg caaaaaaagt ggaagacgta
      301 tcgagatttg tgcgtctgat cgagacatgt ttaaaaatgg cttgccataa ttaacgttgt
      361 atgtgataac agatttcggg ttaaacgagg tacagttctg tttatgtgtg gcattttcag
      421 taaagaagtc ctgagtaaac acgttgacgt tgaataccgc ttctctgccg agccttatat
      481 tggtgcctca tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac
      541 aatctaagga atttttcaaa tggcaaagat taaaggtcag gttaagtggt tcaacgagtc
      601 taaaggtttt ggcttcatta ctcctgctga tggcagcaaa gacgtgttcg tacacttctc
      661 cgctatccag ggtaatggct tcaaaactct ggctgaaggt cagaacgttg agttcgaaat
      721 tcaggacggc cagaaaggtc cggcagctgt taacgtaaca gctatctgat cgaatccact
      781 gatctgaagt gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt
      841 tactatgctt tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta
      901 cacttgcggc ctttagtatc ctgccggagt tgtcatgtcg ttttcctgtc cactttgcca
      961 tcagcctctt acgcgtgaaa aaaacagcta tatctgtccc caacgacatc agtttgatat
     1021 ggcgaaagaa gggtatgtca atctgctgcc cgttcagcat aaacggtctc gtgatccggg
     1081 cgacagcgcg gaaatgatgc aagcacgccg cgcattctta gatgccggac attatcagcc
     1141 gctgcgtgat gcaattgtcg cccaactgcg ggaacggctt aatgaaaagg ccacggcggt
     1201 gctggatatt ggctgtggtg aagggtatta cacacacgca tttgccgatg cgttgcccga
     1261 aatcaccacg tttggtctgg atgtttcgaa ggtagcgata aaagcggcgg cgaaacgcta
     1321 tccgcaggtc actctttgtg tcgcttccag ccaccgtttg ccgttttccg ataccagtat
     1381 ggacgccata atacgtattt acgcgccgtg taaagcagaa gaattagcac gagtagtgaa
     1441 gcccggcggc tgggtcatta ctgccacgcc gggaccgcga catttgatgg agctgaaggg
     1501 gctgatttac aatgaagtac atcttcatgc acctcatgca gaacaactgg aaggttttac
     1561 attacagaag agtgatgagt tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc
     1621 attattgcag atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc aaatactggc
     1681 agcaaaagaa gtgttcgact gccagacgga ctttaatatt cacctctggc agcgttctta
     1741 ttaaccgtgg aagtgcgtcc agaggatctg gacgccgatg ccgatcagca ccagcccgcc
     1801 gagaatttcc gcttttttcc caataattga gccgataaag cgaccaacca tcatccctaa
     1861 tgtcgacata atcaaggttg cacaaccaat ggccaatgcg gtcgcgataa tgttgacctg
     1921 aaggaaagca agaccaacac ccacagccat ggcatccagg ctggtggcaa tcgcggtggt
     1981 taccagtagc cagaaaccgt gccgacggcg cggctcttca tcttcatcat ctgcgccacg
     2041 aaaaccctca ataatcattc gcccgccgag gaatatcagc agcacaaacg caatccagtg
     2101 attccattca aggacaaacc ggctggctaa catgcccatt ccccagccga tcagcggcgt
     2161 cagggtttcg acggcaccaa aaataaggcc ggttcgcaat gcttcagaaa atttcggttt
     2221 atggagggtg gcacctttac cgattgatgc agcaaatgca tccatcgaca taccaaacgc
     2281 aagaagaaca gtagcagtga tattcatgac aacgtcctga ccggggtatc cataaaacac
     2341 atcatcgccc ccagcaaaca tgcatacatg aacaacaagc cgcgatgggt tttcgcgttc
     2401 atgcaggcat agcgattgat gtgcatatgg tctcgcctga ctgaaagtct tcagtccgta
     2461 cgtgccacgt tttgcaacga gtatgttgac acgtacattt ccggaatctg gaaattggct
     2521 actccccaaa tgagggcgca accttaacat attttgagaa tataaaacaa c
//
LOCUS       DAZGXS010000280         2569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGXS010000280 DAZGXS010000000
VERSION     DAZGXS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..335
                     /locus_tag="ACHKG5_004888"
     CDS             <1..335
                     /locus_tag="ACHKG5_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0870806.1"
                     /translation="PEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGR
                     IMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGF
                     LSINKLSQ"
     gene            complement(352..606)
                     /locus_tag="ACHKG5_004889"
     CDS             complement(352..606)
                     /locus_tag="ACHKG5_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0870807.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            716..865
                     /locus_tag="ACHKG5_004890"
     CDS             716..865
                     /locus_tag="ACHKG5_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0870808.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVMRLLDSLKR
                     EGITV"
     gene            1129..2007
                     /locus_tag="ACHKG5_004891"
     CDS             1129..2007
                     /locus_tag="ACHKG5_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFP0870809.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2061..>2569
                     /locus_tag="ACHKG5_004892"
     CDS             2061..>2569
                     /locus_tag="ACHKG5_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0870810.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
BASE COUNT          694 a          548 c          685 g          642 t
ORIGIN      
        1 atccggaagc ggacccggag caggagctga gagaccgctg ggtggtggag cagatgtcag
       61 agctgacggc catgacagcc tcgtttgtgc tggcaacacc gacggagacg gacggagcgc
      121 tgtttcccgg tcgcatcatg ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat
      181 gcggttatca cggtcctgcg gtggcggatg agttcgacaa ccccaccacg gatatccgta
      241 aggacagatg cagcaagtgc atgcgcgggt gtgagatgcg cggcatggtg gctaattttg
      301 gcggtttcct ttccattaat aaactttcgc agtaatggat tatgcccacc atcaggtggg
      361 ttttttatct tcgccgtaca tgctgctgag agtcttaaga agagcctccc taaatttttc
      421 agattcttgt tgagcaaaac cttctattga tcttggtgat ttttcttcat caatagcagc
      481 ttgcaagatc ataactattt ctgaatttaa tgagcgtccg tttttcgatg ctcttatggt
      541 caggtcgcgc ttgaggctat ctggcattct tacgctatat ggggctatgt ctctaacctt
      601 cgtcatactt acaccgtgat aatcacattg atatcacagt gtattcaaaa aaactttgac
      661 ttgatatagt cacagtgata cgtttgtgat tcataataac tcacgcggtg gcgatatgga
      721 aaaagaagtt agcagaattt tggtgagaat acctcagtcg ctaaaagatg cgattacagg
      781 aaaggccaaa gaagaatgcc ggtcgtttaa ctcggaagtg atgcgattgc tggacagcct
      841 gaagagagag gggataacgg tatgagtaaa gaatgttgtt ttttgcggca ttagcgaatc
      901 agacgctgat caaacataca tttactctaa agaaacaggt cggatgctgt gtagtgactg
      961 cgtgtttgac atcataagat acaagtatct tggatgttct gccagcatta gcaatatagg
     1021 tgaagtgtac gaagggaaag atataactga tagagcagaa agttgaagcc ccaactgctg
     1081 taacagtcag ggcttcgtta tcaacaaatc ggcttaggaa atattgacat gaaaagtata
     1141 gcaaaggcac aaaacgattt cactatcttc aaattcggcg acagtgaaat ccgcgtcatc
     1201 aacaagtgcg gtgagccgtg gtttgtagct aaagatgttt gtgatgcttt agctttgact
     1261 aactcacgca aggcgcttac tgcacttgat gacgatgaaa agggagtaac tttaagttac
     1321 acccttggtg gtgagcagaa tctaagcatt gtgagcgaat caggtatgta tacattggtt
     1381 ctgcgctgcc gcgatgcagt caataaaggt tcagtcccgc acaaattccg caagtgggta
     1441 acagcagaag ttctgccttc aattcgcaaa catggcgagt atgtaaaagg aaagaaaacc
     1501 actgttgagg aaagaacacc gctacgcgat gcagtaaaca tgctggtagg aaagaaagga
     1561 cttcgcaatg acgatgcata caatatggtt catcagcgtt ttggtattga cagcattgat
     1621 gagctttcaa ttgaacaaat cccgcttgcc gtagagtaca tccacagggt agtgcttgaa
     1681 ggtgaattca ttggcaaaca agagaagagc accaacgagc tttccgcaaa agaagcaaac
     1741 agccttgtat ggctatggga ttatgccaac cgctcacagg cattattccg cgaactgtat
     1801 ccggcgctaa aacaaattca atcgaactat tccggcagat gctacgacta cggtcatgag
     1861 ttctcgtatg ttattggaat ggcgagagac gttttaatta atcacacacg agatgttgat
     1921 atcaatgagc cagacggacc aacgaatctt tccgcatgga tgagacttaa gaataaagaa
     1981 ttacctcctt caccgcatca ctactaacag attgccaacg aaatgaccca gcttcggctg
     2041 ggtttttatc aggagttctc atgctctata gcaatatatt ggcgcacgcc cggcgatgtg
     2101 caccagcgga gtcgtgcggc ttcgtggtga gaacgccgga aggggaaaga tattttccct
     2161 gcgtgaatat ctccggtgag ccggaagatt atttccggat gtcgccggag gactggctgc
     2221 gggcagaaat gcagggggag attgtggcac tggtccacag ccaccccggt ggtctgccct
     2281 ggctgagcga ggccgaccgg cggctgcaga taaaaagtgc actgtcctgg tggctggtct
     2341 gccgggggga aattcataaa ttccgctgtg tgccacatct gacaggacgg cgctttgagc
     2401 acggggtgac ggactgttac acgctgttcc gggatgccta ccatctggcg ggaattgata
     2461 tgccggattt tgagcgtgag gatgactggt ggcgcaacgg tcagaacctt tacctggaca
     2521 atatggcggt caccggcttt taccgggtgc ccctgtcctc tgcacaggc
//
LOCUS       DAZGXS010000281         2501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGXS010000281 DAZGXS010000000
VERSION     DAZGXS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            321..866
                     /locus_tag="ACHKG5_004893"
     CDS             321..866
                     /locus_tag="ACHKG5_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP0870811.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            841..>2501
                     /locus_tag="ACHKG5_004894"
     CDS             841..>2501
                     /locus_tag="ACHKG5_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0870812.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
BASE COUNT          607 a          597 c          712 g          585 t
ORIGIN      
        1 gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg atggcgtaga
       61 tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc
      121 gacaggttac ggggcggcga cctcgcggtt tttcactatt tatgaaaatt ttccggttta
      181 aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac cccctgaaaa
      241 gaaaggaaac gacaggtgct gaaaacgggc tttttggcct ctgtcgtttc ctttctctgt
      301 ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggcg
      361 cgagtatccg taccattcag aactggcagg agcagggaat gcccgttctg cggggtggtg
      421 gcaagggtaa tgaggtactt tatgactctg ccgccgccat aaaatggtat gccgaaaggg
      481 atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgagg caggacagcg
      541 agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
      601 ccgacgcaca ggaactgaag aatgccagag actcagctga agtggtggaa accgcattct
      661 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      721 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      781 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      841 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
      901 tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgccag ttactatctc
      961 ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
     1021 atgaatgcga tgggcagtga ctacatccgc gaggtgaatg tggtgaagtc tgcccgtgtt
     1081 ggttattcca aaatgctgct gggtgtttat gcctacttca tagagcataa gcagcgcaac
     1141 acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
     1201 ccgactattc gtgatattcc gtcgctgctg tctctggccc cgtggtatgg caaaaagcac
     1261 cgggataaca cgctcactat gaagcgtttc accaatggtc gtggcttctg gtgcctgggc
     1321 ggtaaagcgg caaaaaacta ccgtgaaaag tcagtggatg tggcgggtta tgatgaactt
     1381 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg cgataagcgt
     1441 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
     1501 tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     1561 ccgcactgcg gggaggagca gtaccttaaa tttggcgata aagagacgcc gtttggcctc
     1621 aaatggacgc cggatgatcc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc
     1681 atccgtcagc aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc
     1741 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga accgcctgac
     1801 agtgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt tcagattgtc
     1861 aaagactgga tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg
     1921 ctcggtgaga catgggaagc gaagatcggc gaacgtccgg atgctgaagt gatggcagag
     1981 cggaaagagc attattcagc gtccgttcct gaccgtgtgg cttacctgac cgccggtatc
     2041 gactcccagc tggatcgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc
     2101 tggctgattg atcggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt
     2161 gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc
     2221 cgtatctgct gggatactgg cgggattgac ccgactattg tgtatgaacg ctcgaaaaag
     2281 catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc
     2341 agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc
     2401 gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc
     2461 ggtgccgttc acttcccgaa taacccggat atttttgatc t
//
LOCUS       DAZGXS010000282         2494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGXS010000282 DAZGXS010000000
VERSION     DAZGXS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            58..381
                     /locus_tag="ACHKG5_004895"
     CDS             58..381
                     /locus_tag="ACHKG5_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0870813.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            387..1523
                     /locus_tag="ACHKG5_004896"
     CDS             387..1523
                     /locus_tag="ACHKG5_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0870814.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1520..>2494)
                     /locus_tag="ACHKG5_004897"
     CDS             complement(1520..>2494)
                     /locus_tag="ACHKG5_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0870815.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          615 a          574 c          629 g          676 t
ORIGIN      
        1 atcatggcat ttcataatat gcgttaattg ttctttttta tattctctga gggaaatatg
       61 ttcctggact attttgcact gggagtgctt atttttgtat ttctggtgat attctatggg
      121 attattattt tacatgatat tccctacctg attgctaaaa aacgtaatca tcctcatgcc
      181 gacgccattc atgttgctgg ttgggtgagt ctttttacgc tgcatgttat ctggccattt
      241 ttgtggattt gggccacgct ttaccgcccg gagcggggat ggggaatgca aagccatgat
      301 tcatccgtta tgcaactgca acagcgcatt gccgggctgg agaaacagct cgccgacatc
      361 aaatcctctt ctgccgagta atacttatgg atttactgat tattttgacc tatgtggctt
      421 ttgcatgggc aatgtttaag atcttcaaaa ttcccgtaaa taaatggacc attcccacag
      481 cggccctggg tggaatattt attgtcagcg gtctaattct gttaatgaac tataaccatc
      541 cgtatacctt taaagcgcaa aaagcggtta tttctattcc tgttgtccca caggtgacag
      601 gcgtggtgat agaagtgacg gataagaaaa atacgctgat taaaaaaggt gaggtgctat
      661 ttcgactgga cccgacgcgt tatcaggcgc gggtggatcg gctgatggcg gatatcgtta
      721 ccgcagaaca taaacagcgg gcgttgggcg cagagttaga tgagatggcg gcgaatactc
      781 agaaggcaaa ggccacgcgg gataaattcg ctaaggagta ccagcgttac gcacgcggca
      841 gccaggcgaa agtaaacccg ttttcagaac gcgatattga tgtggcacgg caaaattatc
      901 tggcgcagga agcctccgta aagtcatcgg cggcggaaca aaaacagatc cagagccagc
      961 tggatagcct ggtgttgggt gaacattctc aaatcgccag cctgaaagcg cagctcgcgg
     1021 aagcaaaata taaccttgag cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc
     1081 aagtgctgat tcgtccgggt acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt
     1141 ttatacccga tcagaaacga caaatcgtgg cgcagttccg tcagaactcc ttgctgcgcc
     1201 tggctcccgg cgatgatgcg gaagtggtat ttaacgctct gccaggcaaa gtgttcagcg
     1261 gtaagctggc agccattagt ccagctgttc ccggcggagc ttatcagtcg accggcacct
     1321 tacagacgtt aaacacagcg ccgggttcag atggcgttat cgcgaccatt gaactgaatg
     1381 agcacactga tttgagcgct ttaccagacg gtatttacgc tcaggtggcg gtctactctg
     1441 atcatttcag ccatgtctcg gtgatgcgca aagtgttatt acgcatgacc agctgggtgc
     1501 attaccttta tctcgatcat taatcttcgc tttccgccgc ttgttcaggc cgcagttgct
     1561 gcgactgagc aagctggtgg cgaatttcac gcagcatttc atccagcaca tactggaccc
     1621 gccagggctg gtattcccgt ttacggaaga tagccaccag atccagccac cattcatacg
     1681 ggtgctgaaa gcagggcacc agcgtgccgt cttgcagatc gctctgcaca cttttgtctg
     1741 gcgcaaacac aatccccagg tgatttcgtg ccagctccag cgccgattgc gtgttatcgc
     1801 aaacgtaatt tcccttcacg cgataatccc gcacctcttt actcccggcg acgttgaagc
     1861 gccagatatt cgcgtcgtcg atcatcatcg agtcgatcaa aatacaggag tgatgttcga
     1921 gttcgtcagg acggctaata ggatgtttct caagatagcg ctggctggca taagcggtta
     1981 cggcatattg ggtaataaaa ctggcaacca gcgattcatc ttttggtggg gcatagctga
     2041 ttaaaacatc acaatcatca ggaaattcga cgccttcata aaaggcatta cgctcaagat
     2101 tgcaggtttt tagcgataag gtgatatcac cgatatcttt aattttatcg attacatgtt
     2161 tggataaata ggtaattatg ccggttgggg cgtagatggt gacccgacca cgtttctcat
     2221 gcttataatc tgcaataaaa ttattaagct gctcgtttct gtccagcatg tcgttgatgt
     2281 acggtaatag cgcggtgcca aaaagtgtga gcatcagttg tcgggtggtt cggtcaaaga
     2341 cttttaaacc cacttttgat tcaaaatcag caagatattt gctgacgttg gcctgtgcga
     2401 tgccaagtac agtcgcggca tggctgatat tttcactggc agcgattacc gagataattt
     2461 ttaactcccg gtacttaagt tgtaattttg tcat
//
LOCUS       DAZGXS010000283         2491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGXS010000283 DAZGXS010000000
VERSION     DAZGXS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1235)
                     /locus_tag="ACHKG5_004898"
     CDS             complement(<1..1235)
                     /locus_tag="ACHKG5_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0870816.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAII"
     gene            complement(1497..>2491)
                     /locus_tag="ACHKG5_004899"
     CDS             complement(1497..>2491)
                     /locus_tag="ACHKG5_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0870817.1"
                     /translation="NPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLL
                     KHEDMASFAYENKKLKKLFTLLDKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFS
                     TIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGD
                     TYNLIKTLDVCNYDSERHVLTSHHGIIRDEENKCYCLGALQVPFNQMKNPTDPEELAY
                     IFNKKHKQHMDDHLFHLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDP
                     RKNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
BASE COUNT          764 a          482 c          405 g          840 t
ORIGIN      
        1 ttaataattg ccgctaattc gaccggatcg agagcattgg tcatttcatc aatagagacc
       61 gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc atgatgattt
      121 gccattattt tgtatttgga gtcatagaaa catacaggat aatcttttat cccgtcgtag
      181 gtatcctcat caagtggttt catacgcgtg aagttctgca tatagttacc attgcaattc
      241 gtttcatgat ttccagctaa tacatgaacg ttatcattga tgctcattat gcgttttaac
      301 aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt ttggtcaccc
      361 aaaaacgtac atggcgtcac tgcgtcttta ttaataacta aataaggctg aagactttct
      421 aaaataatgt ctcttctgtt caggatactg attattgtat taacattagt atcaacttgc
      481 tcattaaagt tctcccttag caatacattg agaacttcat cttctatatt aagcaactgg
      541 ctaaggaaat tttctccttc agccatgatt cccaaatgtc ccgttgcaaa aagcgcgtac
      601 aaaactgcac caactgagcc atcggtatca ccaaaatagg ttggtctggt acttgtggga
      661 tcaacaggtg aacaattgga gaaatagatt ctgtcagcga actggggttt tataattgtt
      721 tcgaatttaa tatcagatac attgtattgc gatattttcc agttccagat gggtttgtac
      781 gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt aagtaaataa
      841 acaatgccgc accaattaat atcggtatta tcaagagggc aaccgtaatt tttgctatta
      901 atcgtcacaa taaactgata atggttattc ctacgtagaa tatgagcacg catgcccgat
      961 agcatactat atttttctga ataatccgtt tgtatggtaa atgaaatttt gtcatcttga
     1021 atagattgtt ggaaccgttc tatttgccaa tcatctactt tttccagaac ctcaccgagc
     1081 attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata cccaaacagt
     1141 tctttagtga agttagtaac atgctcttgc accatatcat ttgttgcgat tgtcgttgta
     1201 ctgtcgttag tacctgccac agttccaatt ttcatctttt acctcatata attcaccgcg
     1261 aggaatacat agttatcatt taatcttatg acatacacct catgttcgat ggaaaaataa
     1321 ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt atctcatagt
     1381 agagaaagtc cctaaagatt gtagaggcgt catcagtaca tgcctctaca atcgattaag
     1441 aaaaactatg aaatactggc aggattaaaa agatatacca cagcactcat agagttttaa
     1501 tgtggttgaa aacaacttag tcctgaggaa gaacctatta tttttttatt ttcatcaaac
     1561 actgccatcc tggaatttag ccccataaca ccattatcaa attgttgatt ctgatttacc
     1621 cctaacccat gatggccata tttttgtcta atgtttttgc gggggtcgcc acattcgagc
     1681 gtctgtccac atttgaagat atcttcagga tctggtctga aatcagtagt gttatttaaa
     1741 taatcagcat atacaggtgt gggtgccata gtattgcttc taattagatg gaaaagatga
     1801 tcatccatat gttgtttatg ttttttatta aatatatatg ctaattcctc aggatcagta
     1861 ggatttttca tctgattaaa tggaacctgc aaagcaccga gacaataaca tttattttct
     1921 tcatccctta ttattccgtg atgactggtt aaaacatgcc tttctgagtc ataattacaa
     1981 acatcgagcg ttttaattaa gttatatgta tcaccggaag agagtttatg atttgctagt
     2041 cgtgcactat agttaccatt aaaattgatt tcatgatttc cagccagaac aacgacattt
     2101 ttatttatac gactatcttt attaccctcc atatttcgca tagaatttag caaagtaagg
     2161 atatatttat caccaaaaat agtactgaat ctatcgcctg tatgatcccc aaggaagata
     2221 caaggggtaa tcgcatcgcc ttttaaaaaa acatgttgat gcaactcatt caagatcatg
     2281 tctctcttat ctaataaagt gaagagtttt ttcaattttt tattttcata tgcgaatgaa
     2341 gccatgtctt cgtgtttcaa taaatcacat aataaactcc accccttttc cctgatgcct
     2401 atatgccctg aaacgagcaa tgcataaagt acagcgccta ctcttccatc tgtatcgcca
     2461 aaataagccg tgttggcatt ttgtgggtta a
//
LOCUS       DAZGXS010000284         2481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGXS010000284 DAZGXS010000000
VERSION     DAZGXS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            22..2169
                     /locus_tag="ACHKG5_004900"
     CDS             22..2169
                     /locus_tag="ACHKG5_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0870818.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          722 a          546 c          553 g          660 t
ORIGIN      
        1 tttgtattaa tgagggatga aatgttatat aatatacctt gtcgaattta tatcctttcc
       61 actctgtcat tatgcatttc tgggatagtt tctactgcaa ccgcaacttc ttcagaaaca
      121 aaaatcagca acgaagagac gctcgtcgtg accacgaatc gttcggcaag caacctttgg
      181 gaaagcccgg cgactataca ggttattgac caacaaacat tgcagaactc caccaatgcc
      241 tccatagccg ataatttgca ggacatcccc ggagtagaga taacagacaa ctccttggca
      301 ggccgtaaac aaatccgcat tcgtggcgaa gcatcctccc gtgttttaat tctcattgat
      361 ggtcaggagg taacttatca gcgcgccgga gataattatg gtgtgggact gttgatagat
      421 gagtctgcgc tggagcgtgt tgaggtagtg aaaggtccat attccgtact gtacggttca
      481 caggcaattg gcggtattgt taacttcatc accaaaaagg gaggtgacaa acttgcatct
      541 ggagttgtga aagctgttta taattccgca acagcaggct gggaagaatc aatcgcggtc
      601 caggggagca tcggtggatt tgattatcgc atcaacggta gttattctga tcagggcaat
      661 cgtgatacgc cggatggacg tctgccgaat accaactatc gtaacaatag tcagggtgta
      721 tggttgggtt ataactccgg aaaccatcgt tttggcctct cgcttgatcg ctacagactc
      781 gcgacacaaa cttactatga ggatccagac ggaagttatg aggcatttag tgtcaaaata
      841 cctaaacttg aacgagagaa agttggggta ttctatgaca cagacgtgga cggtgactat
      901 ctaaaaaaaa ttcatttcga cgcgtatgag cagaccatcc agcgccaatt tgccaacgaa
      961 gtaaaaacga cacagcctgt tcccagtccg atgattcagg ctctgaccgt tcataacaag
     1021 actgacaccc atgataagca atacactcag gcggtcacat tgcagagtca cttttcgctg
     1081 cctgctaata atgaacttgt taccggtgca cagtacaaac aagacagggt cagccaaagg
     1141 tccggtggca tgacctcaag caaatctctg accggcttca ttaataagga aacacgaact
     1201 cgctcctatt atgagtcaga gcaaagtaca gtctcactat tcgcacaaaa tgactggcaa
     1261 ttcgccgatc actggacatg gacaatggga gttcgccaat actggctttc ttcaaagttg
     1321 acgcgtggtg acggagtatc atataccgca ggcattataa gcgatacctc tcttgccaga
     1381 gagtctgcga gtgatcacga aatggtaaca tctacaagcc tgcgctattc aggtttcgat
     1441 aacctggagt tacgcgctgc gttcgcgcaa ggctacgtat ttcccacact ctcccagctt
     1501 tttatgcaga catctgcggg cggcagtgtc acatacggaa atcctgatct taaggctgaa
     1561 cactccaata actttgaatt aggtgcacga tataatggta atcagtggct gattgacagc
     1621 gcagtttact actcagaagc taaagattat attgcaagtc tgatctgtga tggcagtata
     1681 gtttgcaatg gtaacaccaa ctcctcccgt agtagctact attattatga caatattgat
     1741 cgggcaaaaa catggggact ggaaataagc gcggaatata atggctgggt tttctcgcca
     1801 tatatcagtg gcaatttaat tcgtcggcaa tatgaaactt caacattaaa aacaactaat
     1861 acaggtgaac cagcgataaa cggacgtata gggctgaaac atactcttgt gatgggtcag
     1921 gccaacataa tctctgatgt ttttattcgt gctgcctcta gtgcaaaaga tgacagtaac
     1981 ggtaccgaaa caaatgttcc gggctgggcc actctcaact ttgcagtaaa tacagaattc
     2041 ggtaacgagg atcagtaccg gattaacctg gcactcaata acctgacaga caaacgctac
     2101 cgtacagcac atgaaactat tcctgcagca ggttttaatg cagctatagg ttttgtatgg
     2161 aatttctgat gacaaaaaca atcgttatgg aattctttgt cctgatctgc ccgctttgcg
     2221 ggcaggctaa cgaacgtatt gtgtttgttg aaggtttttc gacgttgttt tccatctgtg
     2281 ggtgaatatt tattcttccc gccagcatga ctctatactc attgctacga caatgactta
     2341 taaagctggt tactgaacct atacctttgt aattcaaaag tgatatcagt tcacaacgca
     2401 atactatttt tagattattt actctcaccc agatactgaa tcaatggata ataccttttg
     2461 atttatcccg acgcggaaga a
//
LOCUS       DAZGXS010000285         2457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGXS010000285 DAZGXS010000000
VERSION     DAZGXS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..242)
                     /gene="escO"
                     /locus_tag="ACHKG5_004901"
     CDS             complement(<1..242)
                     /gene="escO"
                     /locus_tag="ACHKG5_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP0870819.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKR"
     gene            complement(302..1642)
                     /gene="escN"
                     /locus_tag="ACHKG5_004902"
     CDS             complement(302..1642)
                     /gene="escN"
                     /locus_tag="ACHKG5_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP0870820.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1626..>2457)
                     /gene="escV"
                     /locus_tag="ACHKG5_004903"
     CDS             complement(1626..>2457)
                     /gene="escV"
                     /locus_tag="ACHKG5_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP0870821.1"
                     /translation="IEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIV
                     YKTNKTNQQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKY
                     NELVKELQRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVR
                     IALRRHILGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFL
                     KNIMNPTGNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA
                     "
BASE COUNT          701 a          583 c          452 g          721 t
ORIGIN      
        1 cgtttttgtg aatctagcaa tgaacgcttt tccttcgaaa gtgaagattt tttctcatac
       61 aatcgctgct tttgctcatc aaatgcattg tttttaatct gatatttata caacgacact
      121 ttatccaaat tcaccagcga tgcacaatat gcctgcttag aggcaatcga ctctttaatg
      181 gattgctcgc agtcatcgag tttaccatcc acctctttta tctgagagtt aatcttagcc
      241 attgattctc tcaatcggtt cgctctgctt ttacgaatag ataaaattct gtccaacata
      301 ctcaggcaac cactttgaat aggctttcaa tcgttttttc gtaactactg atatctttgg
      361 tgctttgttg tataaagttc tgaattaatt tcctattttt tattgcctta tctgcttcag
      421 gatcttgccc catcgtgtac tcaccaatac gaataagcag ctcaacattt ttataagttg
      481 caataagctt tttgcattct gccgccgcac gcaaatgctc cgatgtaaca acgttatgca
      541 taacacgact ggcacttaaa ccaatatcaa ttgcagggaa atggttttcc tctgcaagtt
      601 ctcgggtaag tacgatgtgc ccatcaagaa tagagcggac ttcatcgcca ataggatcat
      661 taacattatc gctttctaac aacaccgtat aaatcgcggt tattgaacct tttggcgcag
      721 gccccgctcg ctctaataat ttgggtaacg aagaaaaaac actcggaggg aatccccccc
      781 ttacatcagg ttctcccgat gcaagcccaa catcacgtgc ggctcgggca taacgagtta
      841 cagaatccat cattaataat acatttttac cttgatcgcg gaagtactct gcaatagtcg
      901 tcgccgtaaa tgcggctttc atcctttcca gcgcggggcg gtctgacgtt gtgacgacca
      961 gtacacattt agaaagcgtg gattgaggta agagcgcgag gaattcattt acttcgcgac
     1021 cacgttcgcc gataagagca aggacaataa tatctgcgga tgcgccatta caaatcatcc
     1081 ccaaaagcgt acttttacca acgcctgaac cagcaaaaat accgatacgc tgcccaatac
     1141 cgcaagttag tagcccatca attgccctga cgccaagtat aaatggctga tcaataacct
     1201 gccttaataa aggatctggt ggttcagcat acaagctgcg ttcaaacgga agataggggg
     1261 cagcaatatt actctccatt ggtctgccta tgccatcaac aagccgccca agtagtgcgt
     1321 ctccaacacg aatcttgaac tcatctccct gataacttaa ccactgacca cagtacatgc
     1381 cggaaacatg ctcaaaaggc aacaagaaaa cctcatcttc atcaatagcg atcacctcgg
     1441 ctaaacgttg actgggttct atcttataaa aggcgccaat acgcgctttt ggtaaacgcg
     1501 ccttaataat cgtcccgccg atattgataa tcttgccttc atatctggta gacgaattta
     1561 atgataatgc cggcacagtg ctattgagca ctttctgaat acgtgggtat ttttccaata
     1621 cagaatcatg ctctgaaatc atttaccgtt cctaatactt taagttcgat attattccca
     1681 acctcctgaa aagagagcac ggggactgac gggaacgaac cttcaatcat tttcttcaca
     1741 tagcgcctga tatctaaagc ggtcagaatg acgccgttcc ccgttggatt catgatattt
     1801 tttaagaagc caattatctg ctcagttcgc tccggtgaga tattcagata cgaacctgat
     1861 gacgtttgtc tgatcgactc tcgtagctca ttttcaatat cagagccaat aagccaaacg
     1921 ttcagaagtg taccgctaac gctatagcga cctaaaatat gccgacgcag ggcaatacga
     1981 acgtattcgc acaagataac cacatccttt tcttttgttg accaaaaaat aagcgtctca
     2041 aagatagttc tcaggtctct aattgagaca ttttcctcta cgagacgttg taggatgtca
     2101 acaattttgc tcaaaccaag ctggcgctgc agctctttca caagctcgtt atattttctc
     2161 tccatgatgt ccatcaaata acgcgtttct tgtacgccga tgaactcttt ggcattagac
     2221 aatacaagtt tctttagcaa aaacgttatc ttatcatttg ttgttgcata aacatccata
     2281 cctgtaaggt gagcgagctg ttgattagtc ttattcgtct tatatacgat agagttagtc
     2341 gaaagctttt cctggacaaa ttcgaatgat atctctgcat gcccagcctc aaaatagaca
     2401 gtatcatcta taagagtatc tttgtatatt gactcttgat agagcaaaaa ttctatt
//
LOCUS       DAZGXS010000286         2424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGXS010000286 DAZGXS010000000
VERSION     DAZGXS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1393
                     /gene="escC"
                     /locus_tag="ACHKG5_004904"
     CDS             <1..1393
                     /gene="escC"
                     /locus_tag="ACHKG5_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP0870822.1"
                     /translation="AANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYY
                     DENILYIYKTNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRG
                     VPECIKYITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVS
                     ILKTMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLIS
                     ELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVIS
                     NASNFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESIT
                     SGTLLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNS
                     EMTTEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLF
                     LIKATPIKSASSE"
     gene            1407..1862
                     /gene="cesD"
                     /locus_tag="ACHKG5_004905"
     CDS             1407..1862
                     /gene="cesD"
                     /locus_tag="ACHKG5_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP0870823.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2246..>2424)
                     /gene="grlA"
                     /locus_tag="ACHKG5_004906"
     CDS             complement(2246..>2424)
                     /gene="grlA"
                     /locus_tag="ACHKG5_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFP0870824.1"
                     /translation="NLHYKRLEIFIYDVNLEAVPIESPGTTGPKRKTYRVGNGIVGQS
                     NIWNEMIMRRKKES"
BASE COUNT          810 a          462 c          439 g          713 t
ORIGIN      
        1 tgctgctaac tacagcattc ctgttttcat cagtagttcc gtgaatgatg atttctctgg
       61 cgaaataaaa aatgaaaaac ctgtaaaagt actggaaaaa ctgtccaaac tatatcatct
      121 cacatggtat tatgatgaga acatcttgta tatttataaa acaaatgaaa tatcaagaag
      181 tataattaca ccaacatatc tggatattga ctcactgcta aaatatctga gtgatacaat
      241 ctcagtaaac aaaaactctt gcaatgtgag aaaaattacg acatttaact cgattgaagt
      301 tcggggtgtt cctgaatgta taaaatatat tacatcttta tcagaaagtc ttgataaaga
      361 ggcacagtct aaagccaaaa ataaagatgt tgttaaggtc tttaaactga actatgcgtc
      421 agcgacagat ataacataca aataccgtga ccaaaatgtt gtcgtcccag gtgttgtctc
      481 cattctgaag acaatggcaa gtaatggttc acttcccagt accggtaaag gtgccgtcga
      541 gcgaagcggt aacctgtttg ataacagtgt aacgatttcc gctgatccac gtcttaatgc
      601 agttgttgta aaagaccgcg aaataacgat ggatatctat cagcaattaa tttctgagct
      661 tgatattgaa caaaggcaaa ttgaaatttc tgtctcaatt attgatgttg atgcaaacga
      721 tcttcagcag ctcggcgtaa actggtccgg tacgttaaat gcaggacaag gtacgattgc
      781 ctttaatagc tctaccgcac aggctaatat cagctcttct gtgataagta atgccagtaa
      841 ttttatgatt agggtgaatg cgttacaaca gaactcgaaa gctaaaatac tctctcagcc
      901 ttcaattata accttaaata atatgcaggc gattcttgat aaaaacgtaa cgttttatac
      961 aaaagtctcc ggagaaaagg tggcgtctct ggaaagtatt acaagcggaa cattgctgag
     1021 agtgacacca cgtatacttg atgacagctc gaatagcctt accggaaaac gtcgggaacg
     1081 agttcgtttg ttacttgata ttcaggatgg caatcaatcg actaaccaaa gcaatgctca
     1141 ggatgccagc agcaccttac ctgaagtaca aaattcagaa atgacaacag aagcgacatt
     1201 aagtgcagga gaaagtttat tacttggcgg atttatccaa gataaagaaa gttcaagtaa
     1261 agatggtata ccgctgttaa gcgacattcc tgttattggt agtttattct ccagtacggt
     1321 aaaacaaaaa catagtgttg ttcgactctt tttaattaaa gcaaccccga taaaatctgc
     1381 atctagcgaa taataaatga ggctttatga gcaggaaatt tagctctcta gaggatattt
     1441 atgatttcta ccaggatggt ggcacattag cgtcattaac aaatctgaca caacaagatc
     1501 tcaatgacct tcattcttat gcctatacag catatcaatc tggtgatgtt ataaccgcaa
     1561 gaaatctatt ccatttgctc acatatctgg aacactggaa ttatgactac accttatctc
     1621 tgggcttatg tcatcagcgt ttatcaaatc atgaagatgc acaactgtgt ttcgcacgct
     1681 gtgcaacttt agttatgcaa gatcccaggg catcttatta ttctggaatt agctacttac
     1741 tcgtcggaaa taagaaaatg gccaagaaag cctttaaggc ttgtttaatg tggtgtaatg
     1801 aaaaagaaaa atacactaca tataaagaaa atattaaaaa attgttaggt aatacagagt
     1861 aaaaactacc atcagctata ccaatcccca ggctgactgg ggattttcta tcggaaataa
     1921 taaagctgct atagtgaagt gctcccaaag ttgagcaccg accactggta gatttatcta
     1981 caggtgtggg gttattccat taaccaggag tttatgccct gaactatctt ctgctgtatt
     2041 taatatcatt tccgtttatg aaacaatcag tatatcttaa cttagcatta gaaatctgat
     2101 ctttccaatc atctatagct cagagtatat ttgtcaggaa atgaccttca aactttagcc
     2161 gagaaaaagg cttaccctgg aaaacaaaac ccttaaatat agctttattt ttattcttct
     2221 ataaaatata ctcaaaaaat tacgtctaac tctccttttt ccgcctcatg atcatttcgt
     2281 tccagatatt agactgtccc acaataccat taccaactcg gtaggttttt ctttttggtc
     2341 cggttgttcc aggactttct atcggaaccg cctcaaggtt aacatcataa atgaaaatct
     2401 caaggcgctt ataatgcaaa ttac
//
LOCUS       DAZGXS010000287         2393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGXS010000287 DAZGXS010000000
VERSION     DAZGXS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..185
                     /locus_tag="ACHKG5_004907"
     CDS             <1..185
                     /locus_tag="ACHKG5_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0870825.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004908"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0870826.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHKG5_004909"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHKG5_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHKG5_004910"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHKG5_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          612 a          608 c          677 g          496 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgct gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag gga
//
LOCUS       DAZGXS010000288         2355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGXS010000288 DAZGXS010000000
VERSION     DAZGXS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..671)
                     /locus_tag="ACHKG5_004911"
     CDS             complement(<1..671)
                     /locus_tag="ACHKG5_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870827.1"
                     /translation="MWNLLRRPRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGYHIQANSTSFFRNG
                     GRPSGV"
     gene            complement(617..838)
                     /locus_tag="ACHKG5_004912"
     CDS             complement(617..838)
                     /locus_tag="ACHKG5_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870828.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(883..2355)
                     /locus_tag="ACHKG5_004913"
                     /pseudo
     CDS             complement(883..2355)
                     /locus_tag="ACHKG5_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
BASE COUNT          484 a          775 c          604 g          492 t
ORIGIN      
        1 accccggacg gcctgccgcc atttctgaaa aaagacgttg aatttgcctg aatatgatac
       61 ccctgcgtgg cagccagccc ggcagcatac accggcggca gccccacaag cggatgaaaa
      121 aaacagttaa accggtcgtg gatcacttcc cgggcaggca ccgtcaccgc ctccgtgatc
      181 ccgcagttcc ggtccggcgt gatgcgatag aacacctcgc cgtcatccgc caccagaggt
      241 tcaacccggc tccagtccag aatacgcagt tctttgatct gcccacggga gttacggatt
      301 ttcagcacca ccgtattgcc gtgacgcagt ttggcgttca gccacagttc aaaaaactgg
      361 atacgattct gctgtgcatt gggacgacga cagagacggg caatatcccc ctgccgtttt
      421 tcacggcgga tcccctgtgt atcggtctgc atcaggcgca gccgcatttt ggcgatatcc
      481 tgggatatca gcgaaatgca agaaaacacc gcatgaaagg agaggacact ttccggatcg
      541 gctttcactc cctgctgcca ggcaccggca aaaggctcag ccaccgcctg aaacaggctg
      601 gtccagccca cctcttttac atcacgtcct gatttctggt tttttcgggg tcgccgcaaa
      661 aggttccaca ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgcg
      721 ctgtgatgta ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat
      781 cacggatatc cccggcccgg gcatcatgcg tgccctgcag atactggatt tttgccatca
      841 gttactgcgg gaagctagcg cctcccgccc tcctcatcag actcagccgc cggacgcagt
      901 tccgtagttc acaccggtga tcacagccac cgccgcggta cggcgacgac gccagttgat
      961 ccagcgctcc gcacggatgg ccacgctgcc ggtctggaac atggagacca gctccaccgg
     1021 tgacggtgtg ctgctgtcgc tggtcggttc agactgcatc tccagtgacg cttcacggga
     1081 catatccacc gccacaccac cgtcatccgc cagataaata tccggcgcat tcaccagtac
     1141 cagctggtca cccacatact gggagacaat caccggcagc ccctggaagg tcccgcccag
     1201 cagggtcatg tccggatatt ccttctgccc cagcgcattt ttacgcatgg acagcgccag
     1261 ggcattcgtg ctggacatca gccagaccgc accggtgggc tgcagatttg ccgtcacaaa
     1321 ctggccaaac gcggcctcgg catccgcatc cggattaccg cttgacgccg tgcccttcac
     1381 atcatgggtg atggacgcag gggagacatc tgccaccgcc gcttttttcg ggtccacaaa
     1441 gtctgtatcc agacgcgcca ccaccgcttc cgccagcgca ttacggacca gtgcatcagc
     1501 agccggactg gaaaaacgga tcaattcttc cgtcagtacc gcaatggccg acaccttcgc
     1561 atgactgaag gtgatggatt caaaatcaaa cttcgtcagg ggtctggcct taccctcccc
     1621 cacccagccg gcagctccgc cggacacctg ggcatgcaca cggatattga acggcacctg
     1681 acgaagtgca gggatcccgc cctgaccaaa tcgcccgata atggtctgag gacgcaggta
     1741 atcaataaag tcctgtgcgt attcctgata ttcagacagg ctgcctgccc actgcggatc
     1801 cgtggtggtc cccgcgccac tgccgatttc aggacatgat gcagacgact gtcatccgga
     1861 tactgacgac gggccacttc cagggcttca gagcggacac ctttagccgc agccagcgat
     1921 ttggcaaaac gtgcgaaacc aatcccctta tccagtttct gctccacacg gatcaccggc
     1981 gcagaagcca ccgcggccac attcccgtta ccggcctgtt tcactggctg cgccgtggcg
     2041 gccttaccgg cttccagttc acgcaggcgc ttcaggtgcg catccacctg acgtatttcc
     2101 gctgcggtgt tgtcgtaatg ctcttcctcc tccacatcca gcgtgcgccc ttcctctgcg
     2161 gctttggtca tgacctcctc aagggaggct gccagtgctg cacgcttgtt ttcaaaactt
     2221 ttaatctgtt cgccaatatt cattatggtc ttttccttat gaaaaacggt tgttgactgt
     2281 gccgcagcgc cggcagaaga tgcgattttc accaccggtt tccggttgcc ggacgcggca
     2341 gaaaactggc ggtcg
//
LOCUS       DAZGXS010000289         2301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGXS010000289 DAZGXS010000000
VERSION     DAZGXS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            143..2301
                     /locus_tag="ACHKG5_004914"
                     /pseudo
     CDS             143..2301
                     /locus_tag="ACHKG5_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
BASE COUNT          718 a          536 c          566 g          481 t
ORIGIN      
        1 tcgataaagt tattcaccag gataataacg aaatcccggc aggtctttaa aacagttccg
       61 taataaacat ccgatttcat tcttatatgc cagcaatggc agggatttgt tcacccttaa
      121 atctgtaatg aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca ttccggacga
      181 acaggcaata aaggaagaag gtgcagtcgc tgtagctact gccattgaag ccggtgatga
      241 acgtcgcgcc cgcgcaaaat ttcactggca attcctggaa cattatccgg ctgctcagga
      301 ctgcgcttat aaatttattg tctgcgagga taaacctggc ataccccgcc ctgccctcga
      361 ttcatgggat gctgaatata tgcaggaaaa ccgctgggat gaggattctg cttcttttgt
      421 tccggttgag actgaatccg atccgatgaa cgtcactttt gacaagctgg cccctgaagt
      481 acagaacgct gtcatggtta agttcgacac atgtgaaaac atcaccgttg atatgattat
      541 tagcgcgcag gaattgttgc aggaagacat ggcaacattc gacggacata tcgttgaagc
      601 gttgatgaaa atgccagaag ttaacgccat gtatccggag cttaagttgc acgccattgg
      661 gtaggttaag cataaatgta ttcctggtgc taaatggccc gaaattcaga cagagatgcg
      721 catctggaaa aaacgtcgcg aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt
      781 tgatctcgcc cgcgccagaa ccaatcaaca gtacactgaa aattcaacag gaaaaatcag
      841 cccggtcatt gctgccattc atcgcgaata caagcagaca tggaaaacac tggatgacga
      901 actggcctac gctctctggc ctggtgatgt ggatgccgga aacattgacg gcagcatcca
      961 tcgctgggca aaaaaagaag ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc
     1021 atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc cagactattt ttggccttgt
     1081 ccgtgagcgt ccgatcgaca ttcacaaaga tcccgtagca ctgaacaaat atacctgcga
     1141 atacctgacg acaaagggcg cgtttgagaa tgaagaaaca gacctgggca ctgttgatgt
     1201 tctccagtca tcagaaacac aaactgatgc agtggaaact gaggtatctg atatcccaaa
     1261 aaatgaaacc gcgccggaag ctgaaccatc tgtagagcgt gaggggccgt tctatttcct
     1321 cttcgcagat aaggacggag aaaaatacgg tcgcgcaaac aaactttctg gtctggataa
     1381 ggcactggct gctggcgcca ctgaaatcac aaaagaagaa tattttgccc gaaaaaatgg
     1441 cacatacacg ggcttaccgc aaaatgtaga taccgctgaa gattcagaac aaccagagcc
     1501 gataaaagtt accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc
     1561 tgacgccgat aagttgcttg ctgcatcacg tggtgaattt gttgaaggga ttagtgaccc
     1621 gaatgatccg aaatgggtta aggggatcca gacccgcgat tctgtgaacc agaaccagca
     1681 tgaatcggaa cggaactacc aaaaagcgga acaaaacagt ccaaatgcgt tacaaaacga
     1741 gccagaaacg aaacagcctg aaccagtggc gcaacaggaa gtggaaaaag cctgcaccgc
     1801 ctgcggtcag accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac
     1861 ataccaagaa acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga
     1921 aatggaaggc gctgaacatc cacacaagga gaacactggc ggcaatcagc atcacaatag
     1981 cgataatgaa actggcgaga cggcagatca cccaattaag gtgaacggtc atcacgaaat
     2041 cacatccacc agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa
     2101 tcctgatgcc ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat
     2161 gggaccggaa tttagtaaga ctatcgatct ggaaactgct ggcggagtca ttgatcggga
     2221 caccattaaa tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga
     2281 aatcccgtta gatgatgcac t
//
LOCUS       DAZGXS010000290         2284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGXS010000290 DAZGXS010000000
VERSION     DAZGXS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..845)
                     /locus_tag="ACHKG5_004915"
     CDS             complement(<1..845)
                     /locus_tag="ACHKG5_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0870829.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHKG5_004916"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHKG5_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ACHKG5_004917"
     tRNA            complement(1335..1411)
                     /locus_tag="ACHKG5_004917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ACHKG5_004918"
     tRNA            complement(1425..1501)
                     /locus_tag="ACHKG5_004918"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ACHKG5_004919"
     tRNA            complement(1509..1584)
                     /locus_tag="ACHKG5_004919"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
     gene            complement(1613..>2284)
                     /locus_tag="ACHKG5_004920"
     CDS             complement(1613..>2284)
                     /locus_tag="ACHKG5_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0870830.1"
                     /translation="NIFLEKDTLSLKPGSVVLATKCIRALFLMHYGKVKIFDINHSIV
                     SQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLF
                     ESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGH
                     MFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSR
                     QGLDY"
BASE COUNT          581 a          608 c          551 g          544 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcca gtacgatgac gtagtaccaa
      661 tccggctcag atgaagggcc gacctgtggc tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatgaa aatcaataat
     1621 caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg tgaaatacag
     1681 aaataaagta ggaccagctt gaataaccgc atttttctgc tacagcctgt ccatatccat
     1741 gccgggaaca taacatattg acagcaacac gcatccgctc ttccagcaac aagcgactga
     1801 acatgtgccc ttcatttttc agttttatct ttaacaaact ctcactcata tgcaggcgta
     1861 acgcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc gccctgactt
     1921 tggcacttat actggataaa catccactta aaaataataa tatccgttca tctgattcaa
     1981 acagcgacag gcatgccatc ataagaaaca tatccgtggt atctccggaa aatctctggc
     2041 tggtaattaa agcctcagcc aacgcagggt tgttgggttc cagtgacaga taaagcggaa
     2101 cgtcagtcag atgagttctt gtcagcttat gctgaatttc cagatattga cttactatgg
     2161 aatggtttat atcgaaaatt ttaactttgc cataatgcat aaggaaaagc gccctgatgc
     2221 atttggtggc cagaacgact gagccgggct taagtgacaa cgtatccttt tcaagaaaaa
     2281 tatt
//
LOCUS       DAZGXS010000291         2269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGXS010000291 DAZGXS010000000
VERSION     DAZGXS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..473
                     /locus_tag="ACHKG5_004921"
     CDS             <1..473
                     /locus_tag="ACHKG5_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP0870831.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKLA
                     LSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNILKTI"
     gene            505..1797
                     /gene="sctN"
                     /locus_tag="ACHKG5_004922"
     CDS             505..1797
                     /gene="sctN"
                     /locus_tag="ACHKG5_004922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFP0870832.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1787..2245
                     /locus_tag="ACHKG5_004923"
     CDS             1787..2245
                     /locus_tag="ACHKG5_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFP0870833.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
BASE COUNT          721 a          411 c          542 g          595 t
ORIGIN      
        1 tttcagaggt aattcagcgt ctgatccagg agcgaatttc agtgcgcaat atgcggttag
       61 ttatggaggc cttagcatta tggagcccac gagagaaaga tataatcaca ctcgtcgagc
      121 atgtacgagg tgcattaggt cgctacattt gccataagtt ttcatattct ggagaaatta
      181 aagcaattgt gatttcgcct gaaatagagg acagaatcag agatggtgtc aggcctaccg
      241 ctggcggtac ttttcttaat ctcgatgcat ccgaagctga gatgattctg gataatttta
      301 aactggcact ttctggaatc aatattccta ttaaagacat tattttactg ggatctgtgg
      361 atattcgtcg ttttatcaaa aaacttattg aatctagtta tcgagacctc gaagtacttt
      421 cgtacgggga actaacggaa aatgttcccg ttaatattct gaaaactatt taggataatg
      481 tcttgcgata tggagcatgc tggtatgaaa aaattaaaat tattaaataa atacagctac
      541 ttacatagca taaacggctc tctgattgaa gcagaacttg acgacgtttc tgtgggtgaa
      601 gtatgcgaaa tttatgcgag caggcaagca aatgaacgta tagccagggc tcaggtcgtt
      661 ggctttcgca atgggaagac attattaaac cttataggca gctctgtggg gcttacacgt
      721 actgcagttt tgaaaccaac gggagagcag ttaaccatac agatcagtga tgcctttctt
      781 ggttcggttt tgaatgcctc tggtcaaatt atggaaaggt ttgtcccgga cactccaggc
      841 gataggggaa atttacgcct gattgatgaa ctgccaccgt cgtatcagga acgtcgagtt
      901 attaatacac cacttgagac acggataaga gtcattgatg gcgtactgac atgtggcatt
      961 ggtcaacgtg ttgggatttt cgcctcggct ggctgtggca aaacggtttt aatgcatatg
     1021 ctcgtaaaca atactgaagc tgatgtgttt gttattggct tgattggcga acgtggtagg
     1081 gaagttacgg aatgcgcgga atcgctcaaa aaatcagtaa atgcagcgaa atgcgtattg
     1141 gtctatgcca cctctgattt ttcgtcagta gaccggtgta atgccgccct gatggcgaca
     1201 accgtggcag agtattttcg agatcgagga aagagagttg tactttttat cgactcaatg
     1261 acgcggtatg cacgagcact acgtgatatg aaactggcag cgggtgaacc acctgccaga
     1321 cgtggttatc ctgcttctgt ttttgacagt ttgcctcgct tactggagag gccgggacca
     1381 acactaaaag gaagtattac cgagttttat accgtgttac tggaagggga agatgaatca
     1441 gacccactgg gtgacgaaat tcgctcaatc cttgatggcc atatctatct aagtcgcaaa
     1501 ctcgcgggtc aaggtcacta tccggcaata gatgttctaa aaagtgtgag tcgagtattt
     1561 ggtcaggtca ctgatgaaaa acacagagat aatgctgcac gggtaaggaa gaacctcaca
     1621 actttggaag atctacaggt gtttattgac ttaggggaat atcgcgcagg gcaaaatgct
     1681 gaaaatgatt ttgctatgaa tgcccggcct aaactcacaa attggttgaa acagtcagtt
     1741 aatgaaaaaa tgccaatgag tgaaacgcta aaagagcttg aacgaattgt taagtaaagt
     1801 taatcgactt atcagaagaa cagcgcagag cctggctgca tgtgaagcat cattacaaaa
     1861 gttaaatgca gaaaaagaaa agttagccga aaaggaacgc ctgtatgata tgcaacttaa
     1921 aaacctgcag tcactgttag atatgaagga gttactcggc gaagttgtat ttcgccagga
     1981 tatattttac tcgttacgta aagttacagt cattcagcag cagatcgctg agataaatct
     2041 tgaaaagcaa aaaatagccg agcggcgaaa aatacttaac aaggaaatag ttcaacagca
     2101 agcacaaaga aaacactggt ggctaaaagg agaaaaatac gaccgactaa aaaagcgcat
     2161 caaaaaacaa cttttaaatc agatgcttta tcaagacgag cttgaacagg aggaaaaata
     2221 taatggacga agtcaagaaa attgaacatt gtaaaatttt aaacacaga
//
LOCUS       DAZGXS010000292         2256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGXS010000292 DAZGXS010000000
VERSION     DAZGXS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..2236
                     /locus_tag="ACHKG5_004924"
     rRNA            <1..2236
                     /locus_tag="ACHKG5_004924"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          576 a          501 c          713 g          466 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt tctgtaagcc
      541 tgtgaaggtg tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta
      601 acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa
      661 tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag
      721 gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg
      781 ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca
      841 aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg
      901 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
      961 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
     1021 aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg agctgaaatc
     1081 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
     1141 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggtcagc
     1201 gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
     1261 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag
     1321 gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg
     1381 gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga
     1441 tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg
     1501 aaataccacc ctttaatgtt tgatgttcta acgtggaccc gtgatccggg ttgcggacag
     1561 tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa
     1621 ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact
     1681 gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat
     1741 ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca
     1801 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1861 gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag
     1921 aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg
     1981 ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg
     2041 gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact
     2101 tgccccgaga tgagttctcc ctgacccttt aagggtcctg aaggaacgtt gaagacgacg
     2161 acgttgatag gccgggtgtg taagcgcagc gatgcgttga gctaaccggt actaatgaac
     2221 cgtgaggctt aaccttacaa cgccgaaggt gttttg
//
LOCUS       DAZGXS010000293         2245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGXS010000293 DAZGXS010000000
VERSION     DAZGXS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..376)
                     /locus_tag="ACHKG5_004925"
     CDS             complement(<1..376)
                     /locus_tag="ACHKG5_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP0870834.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVN"
     gene            complement(1011..1472)
                     /gene="yibG"
                     /locus_tag="ACHKG5_004926"
     CDS             complement(1011..1472)
                     /gene="yibG"
                     /locus_tag="ACHKG5_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP0870835.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1484..>2245)
                     /locus_tag="ACHKG5_004927"
     CDS             complement(1484..>2245)
                     /locus_tag="ACHKG5_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0870836.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          633 a          481 c          392 g          739 t
ORIGIN      
        1 tatttactgt ttcaatgcaa tcagcggcaa gttggagtgg atttagattt ttcagatatg
       61 gtcttatgat ctttccctta cttaataaat agcatgtata ggattcggaa agtccagcat
      121 ctattttgta tctcattata ttagataagg atgtgagaaa ttccttgtta tcattcgcag
      181 ccatatatgc cagtccgagt tcagcctctg cggccttaca ggcaaaatag cttagtgaac
      241 acgcttttcg tccctcttta gtactggctg atattagtgc ccataatatt gctttgtctg
      301 ccaaatctaa ctctctctga aattgtcttg cagtaactgc actcgcagaa atagagaaaa
      361 aaatacaatt gttaataacc ttgccatatt ttttagtccg taactgttaa tgtgctatct
      421 ggctctgaaa atattaactc atttaatttt tgcatgtcat tagataaacc agttatacaa
      481 ccctcggaac agaatcgagg tcctaaagac ggaccaaaag cgtttaaaca actatgtatt
      541 gaaaatccat tgcgattata agttcctgta ttaactgacg gtactaatcg tatgccgtca
      601 ggttttctat tgcttgctcc cgggtcatta acattccaac tccataggcc ttgagatatt
      661 ggccatctat ttataaatgt gtacaacggg gattattttt acattgcata ttattaccag
      721 aagcgactgg aatactaagc acatcataaa aattatcatc acatggtata tctgaaacac
      781 acgaaagcat acctaaagtt atgctgtata aacaggtggc taatcccaag gggatcgatt
      841 ccagaaatcg ggttcaacgg atactgataa aaattccacc taccttccag cccaatcgga
      901 gcctgagtgt tataccgccc ctgctgcggt taatgttacc gataatagtt atagtacagc
      961 cctgattcat catcatacta ctgctgcggc agtctgatga actattgctt ttacggattc
     1021 tccttattct tccttctatc cattcgcttt agctttttga gctcttgaag tttgctcttt
     1081 gcatcaatat taccgttatt tacagcgaat tccatccaat aaatagctct attcaaatta
     1141 taatattctg ataatgttgg atttgtatct gataaaaaga ccccataatt aaactgcgct
     1201 gtaacattcc cctgagatgc tgatttttct aagtatcgca attgtttgta aatgttattt
     1261 ttagtaaagc agtaatattg atatagtcga aatgatgcct ctgaatcacc atgttgtgat
     1321 ttatacttca gtttcatcaa cttttcttca cttaaatcat aggtcattgc tgtagacccc
     1381 atcatacttt cgttttgttt tattttcaca tcagcaccat gcaattgaca aataaaagag
     1441 aaataaaaaa atagtaacaa gcatgctttc atacatctac aacctatctt atatcactgg
     1501 gtttttctgg cgtaccttgc atattagaaa tggggtgccc agatggccca tatgttatcc
     1561 aattaccaag gtcttttgtt gttatagcca ttagatcctt atttgaatta aggttcgttg
     1621 gtgctgttag cactgtatcc atttttaact taccattagt tcctcgcccc caccacgtcc
     1681 atagcgtgtc aggtcctatt acatttgtat gtaactcttt agctaattgg ctagcaaatg
     1741 aattttgtcc tttacctgta ttacaagaaa ataaccaaac tgtcatccca tctttataat
     1801 tcccatcaaa tttaattaga tatgctagat ctttagctgt catgatactg cgcgttgcgg
     1861 attcaataga tgtgggggta ccatgccccc cgattgtgaa aacgcctggg atattaatct
     1921 catcagctac agaatggata agatcacttt cggggtataa atttatatca accaaacctt
     1981 gcggatctac atttatgacc ggattcaacg gatactgata aaaattccat ccccccttca
     2041 gtccaatcgg atcctgagtg atatatcgcc cgtgcagcgg gtcataatag cggtggcggt
     2101 tgtaatacag gccggactcc tcatcatact gctgccccgg caggcggata agctgctgca
     2161 gctgatgcgg gttctcttca ttcagcaggt tgccccattc atcgtattct gcgcaccatt
     2221 ctgttgcccc ttccgcactg ataag
//
LOCUS       DAZGXS010000294         2232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGXS010000294 DAZGXS010000000
VERSION     DAZGXS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            9..1367
                     /gene="dgcT"
                     /locus_tag="ACHKG5_004928"
     CDS             9..1367
                     /gene="dgcT"
                     /locus_tag="ACHKG5_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0870837.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1465..>2232
                     /locus_tag="ACHKG5_004929"
     CDS             1465..>2232
                     /locus_tag="ACHKG5_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFP0870838.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPY"
BASE COUNT          655 a          363 c          476 g          738 t
ORIGIN      
        1 gtagctttat ggaaaaagac tatttgagaa ttagtagtac tgtattagtg agcttattgt
       61 ttgggcttgc tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg
      121 ttccacgatc aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat
      181 ttatttttat gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta
      241 gtttggcttt tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa
      301 ttattgaggg gcgtttgaca agcagtgttg tccaaaatga cattgcaatc tattatttgt
      361 ttcgtcagat gagtttgtgc atattaatat ttctggcatt ggtgaataaa gttagtgaaa
      421 acacaaaaca gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg
      481 tttttggggg ggcgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac
      541 acattgccga gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca
      601 taatgatttt catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct
      661 atgatatctg gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc
      721 tgttatttat gagtaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag
      781 tcagtaagtt aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa
      841 caaagaacat tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta
      901 atgaactgac agttcaatca gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg
      961 atatcgacca cttcaaaaaa gtcaacgaca cctgggggca tccggttggc gatcaggtga
     1021 taaaaacagt ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg
     1081 tcggcggcga agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt
     1141 tagcggaaag gattcgggaa aatgttgagc gtttaactgg cgataatcct gaatacgcta
     1201 taccacaaaa agtgacgatt agtattggcg ctgttgtgac tcagggaaat gcgttaaacc
     1261 cgaatgagat ctatcgactg gctgataacg cactatacga ggcgaaagag accgggcgta
     1321 ataaggtggt tgtgagggat gtggtgaatt tttgtgagtc accataaagc ggcattttag
     1381 cgggtgattt tggtaagcgc taaatagcta ttcgcagaca tgcgtaacaa ttttcagaaa
     1441 tatcatagta ctatatgatc ttaaatgctt atcattgatc agtctgctat cacccgcttt
     1501 tactggcaat tgtactataa tttcttaggt tgcatcaccc tgatgcgtgg ggaaacatgt
     1561 atggtattca acaaaaaaat gtttgtactc attataatac caggcattct gggggtgtta
     1621 ctgtcgtttg ctatgtcagt atttcaaatg aatcgagata cgacaattac cgccggtatt
     1681 ctgttaaaac agcttgataa tgttacgcaa attgtcaaac atacgacaaa gcttacttct
     1741 attctggtga tgaaaccctg caaagatatc ctggaacaat tgattgctaa cggagcattg
     1801 accccctatg ttcgaacaac gggtcttata gaaaataatt tccaaatatg ttcttcagtt
     1861 agcggtttca aaaaaatgaa tgttaacgat gtttacggta cgagctttca caataaaaat
     1921 aaagaatcac gcatagtatc aattagcggt acaagttttg taccaggtaa aacagccata
     1981 gtgttcctta tgccaatagg gaatgatatg acagctttta gtattgtaga gtcacgatat
     2041 atctatgatc tgatggatgt tcttgatgat gagaatgatg attcattttc tcttcgcttt
     2101 actgaagggc ctgcaattat tagtggtgta aataataatg acaggttata tatgttaaaa
     2161 aaagatttta attctgcgat cagtcaggct agtcttacag ttactacgcc gatgatttca
     2221 ttatatccct at
//
LOCUS       DAZGXS010000295         2226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGXS010000295 DAZGXS010000000
VERSION     DAZGXS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..259
                     /locus_tag="ACHKG5_004930"
     CDS             <1..259
                     /locus_tag="ACHKG5_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0870839.1"
                     /translation="ASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGYTGGAVAD
                     EFDNPTTDIRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            270..1013
                     /locus_tag="ACHKG5_004931"
     CDS             270..1013
                     /locus_tag="ACHKG5_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0870840.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDGWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            1010..1591
                     /locus_tag="ACHKG5_004932"
     CDS             1010..1591
                     /locus_tag="ACHKG5_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0870841.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1934..>2226
                     /locus_tag="ACHKG5_004933"
     CDS             1934..>2226
                     /locus_tag="ACHKG5_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870842.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT          493 a          542 c          709 g          482 t
ORIGIN      
        1 agcctcgttt gtgctggcaa caccgacgga gacggacgga gcgctgtttc ccggtcgcat
       61 tatgctggcg aatacctgta tgtggaccta ccgctctgat gagtgtggtt acacgggcgg
      121 ggcagtggcg gatgagttcg acaaccccac cacggatatc cgtaaggaca gatgcagcaa
      181 gtgcatgcgc gggtgtgaga tgcgcagcat ggtggctaat tttggcggtt tcctttccat
      241 taataaactt tcgcagtaaa tcccggttta tgacacagac tgaatcagcg attctggcgc
      301 atgcccggcg gtgtgcgcct gcggagtcgt gcggcttcgt gataagcacg ccggaggggg
      361 agtggtatat cccttgtgtg aatatttctg cagagccgga ggcgtatttt cgtatcgcac
      421 cggaagactg gctgcgggca gagatgcagg gggagattgt ggcactggtc cacagtcatc
      481 ccggtgggct gccctggctg agcgaggctg accggcggct gcagataaaa agcgcactgc
      541 cctggtggct ggtctgccgg ggtgacattc acaaattccg ctgtgtgcca catctgacag
      601 gacggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat gcttatcatc
      661 tggcggggac tgaaatgccg gattttcatc gcgaggatgg ctggtggcgc aacggtcaga
      721 acctttacct ggacaatatg gcggtcaccg gcttttaccg ggtgcccctg tcctctgcac
      781 aggcgggcga tattctgctg tgctgctttg gtgcttcggt accgaaccat gccgccattt
      841 actgcggcaa cggtgagctg cttcaccatc tgcctgaaca actgagtaaa cgggagaggt
      901 attccgaaaa atggcaacga cgaacgcatt ctgtctggcg tcaccgccac tggcacgcat
      961 ctgccttcac ggggatttac aacgatttgg ccgccgcctc agcctgtatg tgaacacggc
     1021 agcggaagcc atccgggcgc tgtcgttaca ggtgccgggc tttcgccgtc agatgaacga
     1081 aggctggtac cagatacgta ttgccggtta tgacacggca ccggaggcgg tgtacgcccg
     1141 tcttcacgaa cagctgggtg agggaacggt catccatatt gtgccgcgac tggccggggc
     1201 cggaaagggt ggactgcaga ttgtgctggg ggcggcagcc atcgtgggct ctttcttcac
     1261 tgccggggca tcaatggcgt tatggggttc agccctggca gccggtggtt tttctgccac
     1321 cacgatgctg ttttcacttg gagccagcat gattctgggc ggtgtggccc agatgctggc
     1381 cccgaaggca aaaacaccgg attaccgcgc aacggataac ggcagacaga acacgtactt
     1441 ttcctcgctg gataacatga ttgcccaggg gaacccgatg ccggtgcctt acggggaaat
     1501 gctggttggc tcccgccgta tatcccagga catcagcacc cgtgatgaag gcgggggcgg
     1561 aacggtcgtg gttgtcgggc gacagggata aaacataaaa aaatcccgca gtgatcgcgg
     1621 agctgcgggg acagacaaat gaagatcaat gttaaggagt tgtttttgtt actcgggcaa
     1681 aaaaacacta acgcagcgaa attataagcg ccacagtcag tgtgtgaaaa tgtgaagata
     1741 ttcagaattt ttatgccatt accggtttta accaacagga ttatcggtgg gcatgaaaga
     1801 aaaccccggt atctgctgat accggggttt ctctttagca tggcagaaat gtgtttcatg
     1861 cttttcgggc gaaggatatc cgacttctgt acggaatggc aagtggcggt taatttattc
     1921 aggggaaggc tgtatgggaa aaggtggcgg taaggcacac acgcctcgtg aggcgaagga
     1981 taatctcaaa tccacgcaga tgatgagtgt gattgatgcg attggtgagg gaccgataga
     2041 aggtccggtg aagggactgc agagtattct ggtgaacaaa accccactga cggacacgga
     2101 cggcaatccc gtgatacacg gtgtgaccgc ggtctggcgt gccggggagc aggagcagac
     2161 accaccggaa ggctttgagt cctccggagc tgaaaccgga ctgggcgtgg aagtgacgaa
     2221 ggcaaa
//
LOCUS       DAZGXS010000296         2201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGXS010000296 DAZGXS010000000
VERSION     DAZGXS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            254..469
                     /locus_tag="ACHKG5_004934"
     CDS             254..469
                     /locus_tag="ACHKG5_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0870843.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            474..818
                     /locus_tag="ACHKG5_004935"
     CDS             474..818
                     /locus_tag="ACHKG5_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP0870844.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVAPEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            869..1402
                     /gene="rrrQ"
                     /locus_tag="ACHKG5_004936"
     CDS             869..1402
                     /gene="rrrQ"
                     /locus_tag="ACHKG5_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0870845.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1558..1740
                     /locus_tag="ACHKG5_004937"
     CDS             1558..1740
                     /locus_tag="ACHKG5_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870846.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1753..1884
                     /locus_tag="ACHKG5_004938"
     CDS             1753..1884
                     /locus_tag="ACHKG5_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870847.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            1892..2131
                     /locus_tag="ACHKG5_004939"
                     /pseudo
     CDS             1892..2131
                     /locus_tag="ACHKG5_004939"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2112..>2201
                     /locus_tag="ACHKG5_004940"
     CDS             2112..>2201
                     /locus_tag="ACHKG5_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870848.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPC"
BASE COUNT          545 a          458 c          647 g          551 t
ORIGIN      
        1 tggctaagaa ggatagttgg gtttcacatg atacttatat ctggcagtac attttctgac
       61 agacagtgat gggtgttgtc aagatattgt gtcatttata acctgaatca ggggggggag
      121 ccggaatgtt atctggcatt tttagcagag cctgaatgcc ataatcacgg ctcccggcgt
      181 tggccgtcag tgggcgacac tggcggcttt ttgttttcct ttactttcat tttctgtcgg
      241 cggtgacgga gacatacatc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc
      301 agcggtgggg acgggatact ggttactgca gctgctggac aaagtctctc cgtcccagtg
      361 ggtggcaatc ggtgtgctgg ggagtctgct gtttggcctg ctgacgtatc tgactaacct
      421 gtatttcaaa atcagagagg accgtcgtaa ggcggcgcgg ggggagtaaa gcgatgaaga
      481 aaaaatacga actgggtgtt aaagggataa ataattaccc ggataagatt actgttactg
      541 tggcaccgga aattggtggg tatccgtcac tgttgttgcc agatgtggcg attagtcttg
      601 accgtactga aggtgccacg ctggagtttt acgaagctga ggcgaaaaag caggcgaagc
      661 agtttttcat ggatgttgct gccgggttat gtgaagggga tggtccgttg ccggaaaagc
      721 gccccgtaat tttagaggcg caggatgtgt tgataaccta cagaggaaaa ctaccgggaa
      781 taattacggg ttctctgaag actccaccgc tggcctgaag acttaacata tccagggatt
      841 tgaaatcgat aaaccctgat aaatatccat gaacgcaaaa atcagatacg gcctgtcggc
      901 tgccgttctg gcgctgattg gtgcaggggc gtctgcgcct gaaatcctcg accagtttct
      961 tgacgaaaaa gaaggtaacc acaccacggc ataccgtgat ggtgcgggta tctggaccat
     1021 ctgccgcggt gccatcctgg tggatggtaa acctgtcgtt ccgggcatga agttgtcgaa
     1081 ggaaaaatgc gaccaggtta acgccattga acgtgataag gcgctggagt gggtggagcg
     1141 caatattaaa gtaccgctga ccgaacccca gaaagcgggg atcgcgtcat tctgtccgta
     1201 caacattggt cccggtaagt gtttcccgtc gacgttttac agacgaatta atgcaggtga
     1261 tcgcagggga gcgtgtgagg cgattcgctg gtggattaag gacggtggca gagactgccg
     1321 tattcgctca aacaactgct acggtcaggt ttcacggcgt gaccaggaga gcgcgctggc
     1381 gtgctgggga attgacagat aagcagaata ttttgctgaa aaatgcggtt tgctcacact
     1441 ggcggataac acgaaatcct gcgaactggc aaaaactaag tgaataaaag taaaaccccg
     1501 tttgttggcc gcaaacgggg ttttgtgttt ctgaccttga gtaaggcaag ggagaacatg
     1561 ggaaagtata aacgaattct gttgagattg actatgaaaa acggccttga actgaaagcg
     1621 cctgtaactg atgacgtcag cagagcgctg gcttttgcta ttaagtgggt ggcggtcggt
     1681 attgctgtgt ctccgatgct gtatgggctg gcaaaactgg tcattgcgtt gaaatcgtga
     1741 agaggattaa gcatgtcaga caagctcata acgctggcga agatcctctg tgtaatcgtc
     1801 ggcatttcat tttcactaat gctggttgct atttgctttt ccactgcctg gcgcgtctta
     1861 actttgtcat ggctggtggg gtgaggggga tatgaaccgt gttctgtgtg tggtgattat
     1921 tgtcctgctg gtagcctgtg gtgtgcttag tctggggctg aatcattacc gcgataacgc
     1981 cattacctac aaagcgcaac gcgataaaaa agccagtgag ctgaagctgg cgaacgtgac
     2041 aattactgat atgcaggtac gccagcgtga tgtcgctgca cttgatgcca gatactcgag
     2101 ggaattagcc gatgcgagag ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg
     2161 taagcgcctg cgcatcaacg ccaactgtcc aggctccttg c
//
LOCUS       DAZGXS010000297         2148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGXS010000297 DAZGXS010000000
VERSION     DAZGXS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1679
                     /locus_tag="ACHKG5_004941"
     CDS             <1..1679
                     /locus_tag="ACHKG5_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0870849.1"
                     /translation="NSMAYGEGLPLPETYDRPDPRIKQLARRSTVTPGGVACKYNDII
                     PADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRG
                     GSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQ
                     GEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYFWKQKNE
                     ATYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSA
                     TWTSQDRASHFSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEA
                     TGITTLLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLE
                     YAAGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLA
                     AFYIQTDAKDLNVMYHNAKVATNNLKLGSFGAFDNEWHALAFRFAGNNSLQVTPVIDG
                     QDGTPFTLTQSPVSAFAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            1817..1996
                     /locus_tag="ACHKG5_004942"
     CDS             1817..1996
                     /locus_tag="ACHKG5_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0870850.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            2037..>2148
                     /locus_tag="ACHKG5_004943"
     CDS             2037..>2148
                     /locus_tag="ACHKG5_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0870851.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEAR"
BASE COUNT          549 a          516 c          646 g          437 t
ORIGIN      
        1 caaacagcat ggcatatggt gaaggccttc cgctgccgga gacatatgac cgtccggacc
       61 cgcgtattaa gcagctggcg cgccgcagta cggtgacacc gggcggtgtc gcctgtaaat
      121 ataacgacat cattccggcg gaccattgtc tgcatgatgt gcaggacatg agccgcctta
      181 accatccgaa agcggacctg tcaaaggggc agtacggaac cgtggggcag gggctgcata
      241 tcgccaaaaa attgctgccg tttataccgg cgaatgcggg cattctgctg gttccgtgct
      301 gtcgtggtgg ttcagcgttc accaccggag ctgatggcac atacagtgac gcgagtggtg
      361 cttcggagaa ttcaacccgc tggggtgtgg acaagccgct gtataaggac cttatcggtc
      421 gaacaaaagc agcactgaag aagaacccga aaaatgtgct gtttgccgtg gtgtggatgc
      481 agggggaatt tgattttggc ggtacgccgg caaatcacgc agcacagttt ggtgcgctgg
      541 ttgataaatt ccgtgcagac ctggcggata tggcaggtca gtgcgtcggt ggctctgctg
      601 acggtgttcc ctggatatgc ggggacacga cgtatttctg gaagcagaag aacgaagcca
      661 cctaccagac ggtgtacggc agctacaaaa acaaaacgga aaaaaatatc catttcgtac
      721 cgttcatgac ggatgagaac ggggtgaatg tgccgacgaa caaaccggaa gaagacccgg
      781 acattccggg tatcggatat tacggttcga aatggcgtga cagctcagcc acctggacgt
      841 cacaggacag ggcgagccat ttcagcgcct gggcacgccg tgggattatt tccgaccgtc
      901 tggcaacggc gattttgcgc catgcgggaa gagtggcgct aaacgcgggg gcatcatcga
      961 cagtatcaga ggtgcgcccg tcatcgcctt ccggtgcaga agccacaggc atcacaacac
     1021 tgctctctta ccttgccagc gagtcagagg gaagcctgaa agtacaggga tggtcagcca
     1081 gtggcggcag ggcagaagtg gtcagcgatg cggagggaac cggaggtaag gcagtgaagc
     1141 tgaccaagga agccggtaaa agcagctggg tgctggagta cgccgcgggc aacggtgcgg
     1201 ctctgttaca gaaagggggg cagattcgct gccgctttaa ggtttcggga gcgctggctg
     1261 cgaaccagta tgttatggcg ttttactggc cggtatcttc actgccacag ggcgttgccc
     1321 tgaccggaga cggggggaat aacctgctgg cagcgttcta catccagaca gatgcaaaag
     1381 acctgaatgt gatgtaccac aatgcgaaag tggcgacaaa caacctgaaa ctgggaagct
     1441 ttggcgcatt tgataacgaa tggcatgcgc tggctttccg ctttgccggg aataacagcc
     1501 ttcaggtgac gccggttatt gatggtcagg atggtacacc gttcacgctg acgcagtcac
     1561 cggtcagtgc ctttgcggcg gataaactgc atgtgacaga cattaccaga ggtgcgactt
     1621 acccggtact gatagacagc attgcggtgg aagtgaacag cacagacact gcggcatgat
     1681 aaaaaaaacc gccagcgaca ggaatggacg ctggcggtgg taatacctat ggagaaaaaa
     1741 taaaggaacg atactttcgt gctctggttt tttaaatgaa aacagttctt attgtcaaca
     1801 ataacggaaa gaaattatga catttctgaa ccagttaatg ctgtacttct gtacggtggt
     1861 ctgtgtgctg tatctccttt cgggtgggta cagggccatg cgtgacttct ggcgcagaca
     1921 gattgacaaa agggccgctg agaaaatcag cgccagtcag tcagccggaa gcaaacccga
     1981 agagccgctc atttagcggc aactttctta atcacatctt tcgacgagaa aatcccatgt
     2041 cagaaattac atccctggtc actgctgaag cagtgaagga agtcctgcgc tctgaagaag
     2101 tccggagcgc actgaaacag aaacttcgcc ataacctgga agcgcgtc
//
LOCUS       DAZGXS010000298         2136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGXS010000298 DAZGXS010000000
VERSION     DAZGXS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            138..413
                     /locus_tag="ACHKG5_004944"
     CDS             138..413
                     /locus_tag="ACHKG5_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0870852.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            432..1463
                     /gene="mdtN"
                     /locus_tag="ACHKG5_004945"
     CDS             432..1463
                     /gene="mdtN"
                     /locus_tag="ACHKG5_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0870853.1"
                     /translation="MESTPKKAHRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1463..>2136
                     /gene="mdtO"
                     /locus_tag="ACHKG5_004946"
     CDS             1463..>2136
                     /gene="mdtO"
                     /locus_tag="ACHKG5_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0870854.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNV"
BASE COUNT          463 a          560 c          554 g          559 t
ORIGIN      
        1 aataaaatat ataagttatg aatgcggtgg ctgaataaag ctatttttat tcatcaagaa
       61 aatagccata ctgacgcatt caatggatat tgatattcac tacagatatt gttcgcagca
      121 gacaggatgt atttaatatg ccaacagttc tctctcgcat ggcgatgcaa ctcaaaaaaa
      181 cagcctggat aattcccgtc ttcatggttt cgggatgctc attatctccg gcaatcccgg
      241 tgatcggcgc ttattatccc agctggtttt tctgcgccat tgccagcctt atattgacgc
      301 tcatcacgag gcgagttatt cagcgggcaa atatcaagct ggcatttgtc ggaattattt
      361 ataccgccct ttttgctctc tacgccatgc tgttctggct ggcatttttc taatctattt
      421 gagacgcgct catggaaagt acgccgaaaa aagctcatcg cagtaaattc cctgctctgt
      481 tagtggttgc gttggcgctg gttgcccttg ttttcgttat ctggcgcgta gacagtgcgc
      541 catcaactaa tgacgcttac gtgtcagcag ataccattga tgtggtgccg gaagtcagcg
      601 gccgcattgt agaactggcg gtcaccgaca accaggcagt caaacagggc gatttgctgt
      661 tccgcattga cccgcgcccg tacgaagcca atctggcgaa atctgaagcc tccctcgcgg
      721 cactggataa gcaaattatg ctcacccagc gtagcgttga cgcgcaacag tttggtgccg
      781 actcggttaa cgccacggta gaaaaagccc gtgccgccgc taaacaggcc acagatacat
      841 tacgccgcac cgagccatta ctgaaagaag gttttgtctc agcggaagac gttgaccgtg
      901 cgaggaccgc gcagcgcgct gcagaagccg atcttaatgc tgtattgttg caggcgcagt
      961 cagccgccag cgccgtcagc ggcgtggatg cattagttgc ccagcgtgcg gcggtcgaag
     1021 cggatattgc cctgaccaaa ctgcatctgg aaatggcgac cgttcgcgca ccgtttgatg
     1081 gccgggtcat ttccctcaaa acctccgtcg ggcaatttgc ttctgccatg cgccctattt
     1141 ttaccctaat cgacactcgt cactggtacg tgatcgccaa cttccgcgaa accgatctga
     1201 aaaatattcg ctcaggtaca cctgcaacga ttcgcctgat gagtgacagc ggcaaaactt
     1261 tcgagggtaa agtggattca attggctacg gcgtgctacc ggatgacggc ggcctggtgc
     1321 tgggcggcct gccgaaagtt tctcgttcta ttaactgggt ccgtgttgcc cagcgttttc
     1381 cggtcaaaat catggttgat aaacctgacc cggaaatgtt ccgcatcggc gcttcggcag
     1441 tcgctaatct tgagccgcaa taatgagcgc gctcaactcc ctgccattac cggtggtcag
     1501 gctgctggcg ttctttcatg aagagttaag cgagcggcga ccaggtcgcg tgccgcagac
     1561 cgtgcaactc tgggtaggct gcctgctggt gattctgatc tcgatgactt ttgagatccc
     1621 ttttgtggcg ttatcgctgg cagtgctgtt ttacggtatt cagtcgaacg cgttttacac
     1681 caaatttgtc gcgatcttgt ttgtggttgc cacggtgctg gagatcggca gcctgttttt
     1741 gatctacaaa tggtcatacg gcgaaccgtt gatccgcttg atcatcgccg ggccgatcct
     1801 gatgggctgc atgtttttga tgcgcaccca tcggttggga ctggtctttt tcgctgtcgc
     1861 cattgtcgcc atttatgggc aaaccttccc cgccatgctc gactatccgg aagtggtcgt
     1921 gcgcttaacg ctgtggtgta tcgttgttgg cctctacccg acgttattaa tgacgttaat
     1981 cggcgtactg tggtttccca gtcgtgccat ttcgcaaatg catcaagcgc ttaatgatcg
     2041 gcttgatgat gccattagcc acctgacgga cagcctcgca ccgctacccg aaacgcggat
     2101 tgaaagagag gcgctggcgc tgcaaaaact caatgt
//
LOCUS       DAZGXS010000299         2133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGXS010000299 DAZGXS010000000
VERSION     DAZGXS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHKG5_004947"
     CDS             complement(<1..203)
                     /locus_tag="ACHKG5_004947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870855.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
     gene            complement(361..684)
                     /locus_tag="ACHKG5_004948"
     CDS             complement(361..684)
                     /locus_tag="ACHKG5_004948"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0870856.1"
                     /translation="MIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADINAKANNG
                     FDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPMDLAILMGDKRIIFYLYGYI
                     SHVSE"
     gene            complement(700..1167)
                     /locus_tag="ACHKG5_004949"
     CDS             complement(700..1167)
                     /locus_tag="ACHKG5_004949"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0870857.1"
                     /translation="MIGENAIIYFLIFNMLPIYGGSQWGGGYSDELPDFFSKPKNYHL
                     KSKLNYYPITQAAFLGETEVVSNLIKLGVDLDARGDLGRTALNEAVSNGYFDIVKLLV
                     ESGADLTIKDEFGKTALELAEVHQNYKIVEWLSHYRDHNPIPDFSVLINIYGE"
     gene            complement(1219..>2133)
                     /locus_tag="ACHKG5_004950"
     CDS             complement(1219..>2133)
                     /locus_tag="ACHKG5_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0870858.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKKMHAN"
BASE COUNT          564 a          569 c          360 g          640 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 ttattcagaa acatgagaaa tataaccata taaataaaaa ataattctct tatcccccat
      421 caaaatagcc aaatccatag gggagtatcc catctcactc tccaaataaa tatcagcacc
      481 taatttcaac aaaagcttca tcatatcaaa atcccctatc cctatcgaat aatgcaaagg
      541 agtgaagtca aagccattat ttgccttagc atttatatcg gcaccatgtt gaataaaaca
      601 tctgacatct aattgtcttc ttttcctaac agctatatga agaaccccct cgccaagttc
      661 tgtccttgca tctgtatcta taatctctcc accaaaatat cactcaccgt agatattaat
      721 aagtacagaa aaatcgggta tgggattatg atctctataa tgtgatagcc attcaactat
      781 tttataattc tggtgtacct cagccaattc taatgccgtt ttcccaaatt catcctttat
      841 agtaagatcg gctcctgatt ctactaaaag cttaacaata tcaaaatacc cattacttac
      901 ggcctcatta agtgcggtac gccccaaatc cccacgagca tccagatcca cacccaattt
      961 aattaaatta cttacaacct cagtctcacc taaaaatgca gcctgagtta taggataata
     1021 atttaatttt gacttaaggt ggtaattttt cggcttagaa aaaaaatcag gcaattcatc
     1081 actatagcct cccccccatt ggcttcctcc ataaatcggc agcatattaa agataagaaa
     1141 atatattata gcattttcac caatcaatgg gcataattaa catcattata ttttaaggat
     1201 acctatctcg gaatgccact agttggcatg catctttttt ttaacatcaa taagtgtgtt
     1261 tttcaacgat gctttcacat tttttatttc atccgcatgt ttgccaggcc accatttatc
     1321 atcccattct tgtctcattc ttaaacaatc ttcttttctg ctcttttgaa agatcgcggc
     1381 agcacaacga cttaaattac tatcaggttt ttgctcacaa cgcttcttat atgctaaata
     1441 ttctatttga gcttgcacct cctcctcagc cgttcgcgat ggtcgttgtg tcacactacc
     1501 aagcgtcaaa ccgcccagaa tccccggttt taaaatatcg ccccctaata gcccactata
     1561 cattttcatg ttttgtgaac ccaaaccgtc aatgccatct ctcggaccta attcattaag
     1621 tattttatta tcgggaccac ttcctcctgt aaataatata tcattattac acccttcgga
     1681 atcgcactgc gtcaatccta atgggtcaat ccaacttacc ggattaagcg cataagaata
     1741 taaattccat cctcctttta tatctattgg atcctgggtg atatacctcc cctgtagcgg
     1801 gtcatagtac cggtgacggt tgtaatacag ccccgactcc tcatcatact gctgtcccgg
     1861 cagacggtac ggctgttgca ggtgctgcgc gctggtttcg cccagcaggt ttccccactc
     1921 gtcatactcc ccctgccacg ccgtttcccc ttccgggctg atgagcgcct gcggcagtcc
     1981 ccggtggtcg cagtggtaaa gatgaagttt ccgctccggg atatattccg cctccagctg
     2041 cgcggccatc tgttccgccg tcagcccgca ctgcgcaagc cactgctggc tttcttcact
     2101 cacgctgccc tgccgcagtt cccgctccag cct
//
LOCUS       DAZGXS010000300         2099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGXS010000300 DAZGXS010000000
VERSION     DAZGXS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..2099
                     /locus_tag="ACHKG5_004951"
                     /pseudo
     CDS             1..2099
                     /locus_tag="ACHKG5_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          729 a          414 c          397 g          559 t
ORIGIN      
        1 atcctcagga tttaaaagac agagaaatta tatcaacttt tcacgcagaa aaagtcatta
       61 atgatctgtt aaaaaacgat tataaaattt ccctttctcc agatacaact taccgagagc
      121 tgaataaagc tgcacagcgt agcattacag cgccagacag gataggagaa gggaaaatat
      181 gggtttatca acgagataca atggttgaaa gaggtgataa cagcggtgtt catcagtatg
      241 gtccagctga acatttcact cacattatat ctgacaaacc gtccccaaaa gataagtatg
      301 ttgcatatgc tattaacatt cctgactatg agctagcagc cgatgtatat aatattaacg
      361 tgacgtcacc ttccggacag caagaaacat ttaaaatatt aatcaatcca gaacatctac
      421 ggcaaacact tgagcgtaaa tcccttactg ctgttcagaa atcacaatgt gaaatcatca
      481 cccccaaaaa acctggcgaa gcgattcttc atgcttttaa tgccacctac cagcaaatca
      541 gggaaaatat gtctgagttt gcacgttgcc attatgggta tatacaaatc cctccagtga
      601 caactttccg cgccgacgga ccagaaactc ccgaagaaga aaaaggttac tggtttcacg
      661 cttatcaacc cgaagatctt tgtaccatcc ataatccaat gggagatttg caggatttta
      721 tcgcattggt taaagatgct aaaaaatttg gtatcgatat cattcctgat tataccttta
      781 actttatggg aatcgggggt agtggtaaaa atgacctgga ttatccctct gctgatatac
      841 gagcgaagat cagtaaagat atagaaggtg gtatccctgg ctattggcaa ggtcaggttt
      901 tgattccatt tactatagat ccagtaacaa aagaacgtaa acaaatccat ccagaagata
      961 tacatctcac agcaaaagac ttcgaagcaa gtaaagataa tatttctaag gatgaatggg
     1021 aaaacctcca tgcattaaaa gaaaagcgtt taaatggaat gcctaaaacc ccccccaaaa
     1081 gtgactaggt tattatgttg caaaatcaat acgttcgtga aatgcgaaaa tatggcgtac
     1141 gaggtttacg ttacgatgca gcaaaacact caaaacatga acaaatagaa agatcaataa
     1201 ccccaccgct taaaaattat aatgagcgat tacacaatac taacttattt aacccaaaat
     1261 atcatgaaaa agccgttatg aattacatgg aatatctggt aacttgccag ttggatgaag
     1321 aacaaatgtc atcgctgctt tatgaaagag atgatttaag cgccattgat ttttcattgc
     1381 tcatgaagac gataaaagct ttttcatttg gtggcgatct ccaaaccctt gcatcaaaac
     1441 cgagttccac aatctcaagc atcccatcaa aaagacggat attgattaac attaaccacg
     1501 attttcctaa caatggcaat cttttcaatg actttctatt taaccatcaa caagatgaac
     1561 aattagcaat ggcatatatg gccgctctcc cgttcagcag gcctttagtt tactgggatg
     1621 gccaagtatt aaaatcaacg actgaaatta aaaattatga tgggtcgacg cgtgtcggcg
     1681 gtgaggcgtg gcttaataaa ggttgctcta cctatcagca gctctacaat gaattccacg
     1741 cattatatat agataaagca ggaatatgga gcgcatttga gggtgtattt gcaactaaaa
     1801 acgttctggc ctttagtcgt ggggattcgg tgaacattaa tcactctcct catgatggac
     1861 tagttataat aaataaagga aacgaagaag ttgaaggtgc ctggcctaac aaattgcaac
     1921 ctggaaaata taaaaacatg gggagtaata gcgttaacat tattattaat aatacccgaa
     1981 aaattatccc ccctggtaaa gcatttatgc ttagaggcgg aactctaaat atcaatattc
     2041 ctgggcgtag cgctcttctt ttagggaaaa caggagaacc gctgaactat ctctatttg
//
LOCUS       DAZGXS010000301         2084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGXS010000301 DAZGXS010000000
VERSION     DAZGXS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>2084
                     /locus_tag="ACHKG5_004952"
     CDS             <1..>2084
                     /locus_tag="ACHKG5_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0870859.1"
                     /translation="TAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKPGIPR
                     PALDSWDAEYMQENRWDEGAASFVPVETESDPMNVAFDKLAPEVQNAVMVKFDTCENI
                     TVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKW
                     PEIQAEMRIWKKRREGERKETGKYTSVVDLARARVHRQHTENSAEKIPPVTAVIRREY
                     KQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQPDA
                     LRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVP
                     ETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAA
                     GATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKKIMQAANISQPDA
                     NQLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQKAEQNSPNTQQNE
                     PETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDP
                     EEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITSTSRTCDHLMIDLET
                     MGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQS
                     AIMTDEIPLDDAL"
BASE COUNT          644 a          509 c          528 g          403 t
ORIGIN      
        1 actgccattg aagccggtga tgaacgtcgc gcccgcgcaa aatttcactg gcaattcctg
       61 gagcattatc cggctgctca ggactgcgct tataaatttc ttgtttgcga ggataaaccc
      121 ggtatacccc gccctgccct cgattcctgg gatgctgaat atatgcagga aaaccgctgg
      181 gatgaggggg ctgcttcctt tgtcccggtt gagactgaat ccgatccgat gaacgtcgct
      241 tttgacaagc tggcccctga agtacagaac gctgtcatgg ttaagttcga cacatgtgaa
      301 aacatcaccg ttgatatggt gattagcgcg caggaattgt tgcaggaaga catggcaaca
      361 ttcgacggac atatcgttga agcgttgatg aaaatgccag aagttaacgc catgtatccg
      421 gagcttaagc tgcatgccat cgggtgggtt aagcataaat gtaagcctgg tgccaaatgg
      481 cccgaaattc aggcagaaat gcgcatctgg aaaaaacgtc gcgaaggtga acgcaaggaa
      541 accggaaaat acacgtctgt tgttgatctc gcccgcgcca gagtccaccg acagcacact
      601 gaaaactcag cagaaaaaat cccccctgtc actgcagtca ttcgtcgcga atataagcag
      661 acatggaaaa cactggatga cgaactggcc tacgctctct ggcctggtga tgtggatgcc
      721 ggaaacattg acggcagcat ccatcgctgg gcaaaaaatg aagttatcga caacgaccgc
      781 gaagactgga agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct tcgctacgac
      841 cgccagacta tttttggcct tgtccgtgaa cgtccgatcg acattcacaa agaccctgtg
      901 gcactgaaca aatacattac tgaatacctg actacaaagg gcgtgtttga agatgaagga
      961 acaaatcaga gcgcaactga cactctctcg tcgccagtac cagaaactga tgcagtggaa
     1021 acggcaattc cggacaacga aaaaaccgaa tgcaaagtgg aagtcgaacc atctgtagag
     1081 cgtgaggggc cgttctactt cctcttcacc gacaaggatg gcgaaaaata cggtcgcgca
     1141 aacaaacttt ctggtctgga taaggcgctg gctgccgggg ctactgaaat cacaaaagaa
     1201 gaatattttg cccgaaaaaa tggcacatac acaggcttac cgcaaaatgc aaataccgca
     1261 caaaattctg aacaaccaga accggtaaaa gttaccgctg acgaagtaaa gaaaattatg
     1321 caggcagcca atatcagcca gcctgacgcc aatcagttgc tcgccgcatc acgtggtgaa
     1381 tttgttgcag ggattagcga cccgaatgat ccgaaatggg tgaaggggat tgaaacccgc
     1441 gattctgtga accagaacca gcaagaaacg gaacagaacg accagaaagc ggaacaaaac
     1501 agcccaaata cgcaacaaaa cgagccagaa acgaaacaac ctgagccagt agcgcaacag
     1561 gaaccggaaa aagtctgcac cgcctgcggt cagaccggcg gcggcaactg ccctgattgt
     1621 ggcgcggtga tgggcgacgc aacataccag gaaacattcg atgaagagta tcaggttgaa
     1681 gttcaggaag atgatccgga ggaaatggaa ggcgctgaac atccacacaa ggagaacact
     1741 gacggcaatc agcatcacga tagcgataat gaaactggcg agacggcaga tcactcaatt
     1801 aaggtgaacg gtcatcaaga aatcacatcc accagcagga cgtgtgacca tctaatgatc
     1861 gaccttgaaa ccatgggaaa aaatcctgat gccccgatca tctcaatagg tgcaatattt
     1921 ttcgatccgc aaaccggaga tatgggaccg gaatttagta agactatcga tctggaaact
     1981 gctggcgggg tcattgatcg ggacaccatt aaatggtggc ttaagcaatc acgcgaagcg
     2041 caatctgcca ttatgaccga tgaaatcccg ttagatgatg cact
//
LOCUS       DAZGXS010000302         2083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGXS010000302 DAZGXS010000000
VERSION     DAZGXS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..147
                     /locus_tag="ACHKG5_004953"
     CDS             <1..147
                     /locus_tag="ACHKG5_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0870860.1"
                     /translation="LLQLLDKVSPSQWVAIGVLGSLLFGLLTYLTNLYFKIREDRRKV
                     ARGE"
     gene            complement(403..675)
                     /locus_tag="ACHKG5_004954"
     CDS             complement(403..675)
                     /locus_tag="ACHKG5_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870861.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            835..1368
                     /locus_tag="ACHKG5_004955"
     CDS             835..1368
                     /locus_tag="ACHKG5_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0870862.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1589..1702
                     /locus_tag="ACHKG5_004956"
     CDS             1589..1702
                     /locus_tag="ACHKG5_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870863.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1704..2021
                     /locus_tag="ACHKG5_004957"
                     /pseudo
     CDS             1704..2021
                     /locus_tag="ACHKG5_004957"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1966..>2083
                     /locus_tag="ACHKG5_004958"
     CDS             1966..>2083
                     /locus_tag="ACHKG5_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0870864.1"
                     /translation="MVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQTPLNG"
BASE COUNT          536 a          428 c          574 g          545 t
ORIGIN      
        1 ttactgcagc tgctggacaa agtctctccg tcccagtggg tggcaatagg tgtgctggga
       61 agtctgctgt ttggcctgct gacgtatctg actaacctgt atttcaaaat cagagaggac
      121 cgtcgtaagg tggcgcgggg agagtagtcg atgaataaac aatacgaact ggttgtaaaa
      181 tgaatatttc taactgaaaa aacgttccat gaggtaagaa aaggtcacag gcaatcaata
      241 acaggacgtg atgaaagacc cttgcatttg tgcgctttct ctttagatag cagcagatac
      301 tgaaaatctg agttgtcggg gagtcaggga tacagctgtg caagagttgg tcattgtgat
      361 tccattgaaa tcctgtatgc catgaagggc aggattttat ggctacctga gctttggtga
      421 tagtaagttg aaaattcgca ttttttgctg acatgcgtaa cgagaatccc ataagcaggg
      481 aggacttaat tcttcattaa cccatgcgtt gatattatgt ttcagccgtt gaagcatcag
      541 cggtgttaat gttgtggtaa taatatccag cgttttatgt gagatcttac cgtaagggtc
      601 tgcaagaatg ctgcttgttg cttcgttatt atctgccatc agaagaagta actctgattt
      661 aacgttttct gtcattagtt gtaaaaatct tctgcgcaaa ctttctttac tgttcattta
      721 tatggcttca tttgttgtaa tctgctgcgt ctcaagggat atgtttatga gagcgaccat
      781 gagtgttgga ttatatacct aacatatcaa gggattagaa atcgataaat ccccatgaac
      841 gaaaaaataa aatacggcct gtcggctgcc gttctggcgc tgattggtgc aggtgcttct
      901 gcgcctgaaa tcctcgacca gtttctggat gaaaaggaag gtaaccacac cacggcatac
      961 cgtgatggtg cggggatctg gaccatctgc cgtggtgcca ttctggtgga tggtaagcct
     1021 gttattcctg gcatgaagct gtcaaaggga aaatgcgacc gggttaatgc catcgaacgt
     1081 gacaaggcgc tggcatgggt ggagaaaaac atccgggtgc cgctgaccga accccagaaa
     1141 gcggggatcg cgtcattctg tccgtacaac attggccccg gtaagtgctt cccgtcgacg
     1201 ttttataaac gaattaatgc aggcgatcga aaaggtgcct gtgaggcgat tcgctggtgg
     1261 attaaggacg gtggcagaga ctgccgtatc cgttcaaata attgctacgg tcaggtctca
     1321 cggcgtgacc aggagagcgc gctggcgtgc tgggacatcg acagatagca gaatattttc
     1381 ctgaaaaatg acgttggcca acgcgggtgg ataacacgaa atcctgaaaa ctggtaaaac
     1441 ctaagtgaat aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt gtgttttctg
     1501 accttgagta aggcaaggga gaaattatgg gtagggaggt actttccctg tgaggaagta
     1561 taaaagattc tttctgaggt tgtccattat gaaaggcatt gaagtggaga cgccagccag
     1621 tctggattta acaagagcgg cagcttttgc cattcgtatt gtggccattg ctgttctggt
     1681 ctgggcaatc cgttggtggt gatatgaacc gtgttctgtg tgtggttatc attgtcctgc
     1741 tggtggcctg tggtgcgctt agtctggggc tgaatcatta ccgtgatcac gccatcatct
     1801 acaaagagca gcgcgataaa aaagccagtg agctggagct ggcgaacgcg acaattactg
     1861 atatgcagat acgccagcgt gatgtcgctg cacttgatgc cagatactcg agggaattag
     1921 ccgatgcgag agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc
     1981 tgcggatcaa cgccacctgc tccggtaccg tgcgtgaagc caccggcacc tccggcgtgg
     2041 ataatgcaac cggcccccga ctggcagaca ccgctgaacg gga
//
LOCUS       DAZGXS010000303         2079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGXS010000303 DAZGXS010000000
VERSION     DAZGXS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..209
                     /locus_tag="ACHKG5_004959"
     CDS             <1..209
                     /locus_tag="ACHKG5_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0870865.1"
                     /translation="KGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAPK
                     RPPGPTPIELLKQEYERRKAAGFV"
     gene            244..942
                     /locus_tag="ACHKG5_004960"
     CDS             244..942
                     /locus_tag="ACHKG5_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0870866.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            964..1188
                     /locus_tag="ACHKG5_004961"
     CDS             964..1188
                     /locus_tag="ACHKG5_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870867.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1185..1541
                     /locus_tag="ACHKG5_004962"
     CDS             1185..1541
                     /locus_tag="ACHKG5_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFP0870868.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1574..1726
                     /locus_tag="ACHKG5_004963"
     CDS             1574..1726
                     /locus_tag="ACHKG5_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870869.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1723..2034
                     /locus_tag="ACHKG5_004964"
     CDS             1723..2034
                     /locus_tag="ACHKG5_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870870.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          621 a          408 c          589 g          461 t
ORIGIN      
        1 caaaggggat tgagcgccag atgaccgaag gggagttaaa acggcttgtg gaaaggcagc
       61 tgacgaaatg ggcaaagcat gttggtaatg gcctcagtgt tccgccagtc cggcgacaac
      121 tggcagcacc caaacgcccg ccagggccaa cgccaattga attgctgaaa caggaatatg
      181 agcgccggaa ggcggccggt tttgtctgag ttgagaagta atttttaccg ggaggaaaat
      241 ttaatggaaa ccgtttttga cgcactgaaa gcactgaaaa aagcctcgtc gcacgagatt
      301 gcagcccgtc ttgaaatcag ccgtgacgat gctgttaccg aactctggaa gctgaagcgt
      361 cgtggcgaag ctgataacaa ggggtcgatg tggtggctga cgagtgaggc aactgaagcg
      421 gccccaaaaa ccactgcaga gatgctgatt aacgcgattg aacagcatgg tcctcagtcg
      481 gctgacgaac tggcgttaat gttcgggatt acctcccgcc gggcgaattc atcactggcg
      541 atggctgtca gcaaaggtcg tctgattcgc gtaaatcagg acggtaaata tcgttactgc
      601 ataccgggcg ataatttacc ggcagagccg aaagctgcat cggtaacgga aaccgatggt
      661 aaagcctttc ctcagccggc aggtgttgcg ttaccagtcc gggaagcgga aacacaggaa
      721 gaaattaaaa ctgaaagtgt ggcggtcaca gtgcagtcac agtcgtcgtt catcagaaag
      781 catccggatg gtctgatttt accatcgctg catgtggcta accgcgagct gcgccgggca
      841 aaaggtcagg ttcagaagtg ggagcgctta tgcgccgcgc tgcgggaaat taataagcat
      901 cgtgatgtca tacagaaaat aacgacagga gataacgggt aatttttaca gcagggtgat
      961 gatatgaaaa tcagatatca tgatttcggc cccgtgtcac atatgcttat ttcaagtacg
     1021 gtgctggaaa caagaaaaca taatcacata ctggatatgc tgcgtcttgc tgacccgtat
     1081 ctggtaataa acaccagtgg aattttcttt ctgagaagca ctgtgtcagg aaaaacatcg
     1141 catgtgcttc gggcatataa aacagcagtg cgggaggaag gagaatgagc gagattaact
     1201 atcaggagct gcgtgttgag ctgggagcag caaaaaaacg catagaagag ctggaggcta
     1261 atcgtgtggt gcttgaggtg gaaaatgaga ggttgaaaca cgcaatggcc gtagcccttg
     1321 agcatatatc cgtcacagat gcaggacagg caggtgtcgc tgcaatgatt atctatgatg
     1381 ccatgtacca cagtgaaaaa actgacccct acgcttttct ggcagaattg cgggcgcagg
     1441 gtgtggagat ggtgcgcgaa catccagcaa tcaaactttg ctctttgacg cacatatgtg
     1501 acgagttagt cgcagagctt cgcaaaggag gcaacaagtg agtaaaacga atccgggctg
     1561 ggcaaggcca cttatggcga aaaaacatca ttattttgct gaaggtgaaa taacaagtat
     1621 ttgcggtggg tggatgtatt ttggcaatga gcgtgagccg gatacattcg aaagtccaga
     1681 tgattgtaaa aagtgtcgta gaaaattaaa taaggggggt aaatgagcgt aattaaagaa
     1741 atgccagtgg aacgtaatga atatggctgc tggacgcatc ctgaatatga aaaattctgt
     1801 gatggtcggg aatatatttc aacggaagag tttaacgcct ggatggagaa aaataatctt
     1861 cagtgggcta tccgtagtat ggatgaagat gattttaatc tggacgcaga tggccctgat
     1921 atttcagcat gggaacctga gcgaccagaa ggtgagggat ggtttatcgg ttcgatacat
     1981 gacaccgaag atggtcctgt ttgtgtatgg ctgagaaata aggccgaagc ataaaggcta
     2041 taaaccaaca actaaacact gaaaatttaa atcagaaat
//
LOCUS       DAZGXS010000304         2078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGXS010000304 DAZGXS010000000
VERSION     DAZGXS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..506
                     /locus_tag="ACHKG5_004965"
     CDS             <1..506
                     /locus_tag="ACHKG5_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0870871.1"
                     /translation="REPRAAMALRKIAGLYRIEKFIRERPVEKIRQWRQRYSRPIVND
                     LFAWPEEQEPCCPPDGPLNKAINYILNRRDELSCFLSDGAVPLDNNICERAIRPVVMG
                     RKAWLFAGSLMAGNRAAQIMSLLETAKRNGLEPHAWLTDVLTRLPEWPEDRLEELLPL
                     EGFTFSG"
BASE COUNT          568 a          528 c          511 g          471 t
ORIGIN      
        1 gcagggagcc ccgtgcagcc atggcgctga gaaaaatcgc gggtctttat cgcattgaga
       61 agttcatccg tgaacgtcct gtggaaaaaa tacggcaatg gcgacaacgg tattcccggc
      121 cgatagtgaa tgacctgttt gcctggcctg aagagcagga gccgtgctgt ccgcccgacg
      181 gaccactaaa caaagcgatt aattatatcc tgaaccggcg cgatgaactg agctgcttcc
      241 tgagtgacgg agccgtgccc ctggacaaca atatctgcga gcgtgctatc cgcccggtgg
      301 tcatggggcg gaaagcatgg ttattcgccg gttcactgat ggccgggaac cgcgcggcac
      361 agataatgag ccttctggaa acggcaaaac gtaatggtct ggagccgcat gcctggctga
      421 cagacgttct gacgcgtcta ccggagtggc cggaagaccg actggaagag ttgctgcccc
      481 ttgagggatt taccttctcc gggtgagtga tacctgccat caggtgttcg tgcagcgggc
      541 cataacccgc agtcgggagt tgaacgcctg acgacaggaa atgagccaga gcagcgacca
      601 cgcgggctga cagtgagtca gaacctgaat cagcccggag ccattcccgg ggctgtactt
      661 catcacctgc catcagtacc cgtgtcagta cttcgcgacc tgacgcaatt acccgactca
      721 gttcttcaga acccgcctcc gataccagcc tggcggtcgc ccctgtgcaa caaaataaat
      781 caaccatcgt cagacggtga tcaatcgtat gcgctgccag tggctcgcct gaaaaatgcg
      841 tcaatatgag ttcgccggaa caatacctca gttcttcaga acccgcctcc gataccagcc
      901 tggcggtcgc cccggcgcaa cagaataaat caaccatcgt cagactgcga tcattcgtat
      961 gcatcgctga tgatgcgccc gaaaaacgcg tcaatatgag ttcgccggaa tattactgac
     1021 gacacggttt gtagttacgt tccttcggtg agtgagtacc tgtgcgtcag ggtaacgcaa
     1081 tcagttcagg tttagcgggg tgtcaagtcg gtttggctaa ccgaccgcag aattaactgt
     1141 acctttaaag gtcgcccccg atatatctgc ctgtcagaca tatcgccgat tcggtatctc
     1201 actacataaa actccttaaa aaacaatgcg attctgttgg tacgaagctt tcagtaagtc
     1261 agtccggaga agcgccagta cgatacagaa tgaggttgaa aaaaacgttc acgtctggtc
     1321 acttcttcaa atgttaaaaa acatctcgta aagagccagt ttatgcgggt tcgttgagta
     1381 gacgtgcgag cgtcactttg tcagctttaa cgctgacgaa agtgacgccc gcacagcttc
     1441 aaactccaca tgcacacgca agcgaccaaa tttcaaatgg tcttatgccg tgcgcacagt
     1501 cataagaagc gcatgcggaa agtcagaaaa tatcttaaga cgcagatttt aaaggcgttt
     1561 ttatgccagt ctgccgggaa tacacagtag tccggttgtc cggaagcacc acggtaatcc
     1621 gaaggaaaaa tcaacgtacc tcaatcaccg tagtgatatc cgttcccaga ggctaaacgg
     1681 atgtaccacc ataaaggcat cctctttcag gctacgagga tattgcaatc acccgaaggc
     1741 gtttctctca gattgctgaa acattggctg gcagtcaccc gaaggcgtcc ctctcagact
     1801 gctggaacat tggctggtcg caaaagcgac agttaatgca aatacaggtt aacaaaaagg
     1861 gaaatctgcc gtcaacccaa ccatatgaac atgttgattt cattatgaat gtgatgttct
     1921 aatataaatt aatcagtaac ttcataaaaa gaatataaga aacagataat gcatgtattt
     1981 acacacatat cagcacaatg atagattacc accccagaaa cacgataaag gtataattat
     2041 ggaattttcc gttatttttt tctgggttat cactggtc
//
LOCUS       DAZGXS010000305         2074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGXS010000305 DAZGXS010000000
VERSION     DAZGXS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..577
                     /locus_tag="ACHKG5_004966"
     CDS             <1..577
                     /locus_tag="ACHKG5_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0870872.1"
                     /translation="SLDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDL
                     QYFKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLES
                     QAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNV
                     NLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            591..830
                     /locus_tag="ACHKG5_004967"
     CDS             591..830
                     /locus_tag="ACHKG5_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFP0870873.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            850..1182
                     /gene="sctI"
                     /locus_tag="ACHKG5_004968"
     CDS             850..1182
                     /gene="sctI"
                     /locus_tag="ACHKG5_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP0870874.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1179..1913
                     /locus_tag="ACHKG5_004969"
     CDS             1179..1913
                     /locus_tag="ACHKG5_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP0870875.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            1929..>2074
                     /locus_tag="ACHKG5_004970"
     CDS             1929..>2074
                     /locus_tag="ACHKG5_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP0870876.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSV"
BASE COUNT          707 a          348 c          405 g          614 t
ORIGIN      
        1 tagtttggat gaagcagtct gggtcaaaca agcactggaa aaaaatcaac caggaaagcc
       61 agtacgggtg ataaatcctg atgatgaatc gatacgaatt ttttcatggc ttgctgataa
      121 tttccctgat ttacaatatt ttaaacttca gctattagat gccagtaatc ccagactgac
      181 cgtgagtaag caacggaatg ctatcacaca gcaactaatc gacaatctta ttaaagggtt
      241 actacaaact atgccatatg ccagcaatat aagtattgcg gtattagatg ataatgtatt
      301 ggaaagtcag gctattgaaa cattgtctgc aataggtctt tcttatgaaa aatataaaac
      361 agctaacaat gtgtatttca atatcattgg tacgttaagc gacagtgaat taaacaaaat
      421 aaataactat gttgacgaat attataaaca atggggtaaa caatatgtaa gatttaatgt
      481 gaatttgaaa aatcaggaca caaataatag ttcatttagc tacggagata accgattcga
      541 gaagtctcaa ggtagcaagt ggacatttca ggaataattg aggaggactt atggcagatt
      601 ggaatggtta tattatggac atcagcaaac aatttgatca gggcgttgat gatctgaacc
      661 agcaagttga aaaagcgttg gaggatttag caaccaatcc ttccgacccg aaattccttg
      721 ccgaatatca aagtgcatta gctgagtata cattatatcg aaatgcgcaa tctaacgttg
      781 taaaagcgta taaagatctt gattcggcaa tcatacaaaa cttccgctaa actggataag
      841 gattttatta tgtcagtaag caatatgcca cctattgata gagctgagca atcaactgct
      901 catgaaatac agcaagcgaa agtgattgat ttaaatgacc gggttctcaa tcttgacaac
      961 ccggatgata aaatgatctc cgcttttgct aactatgctg tacaaactga aaattggcag
     1021 caaaatgcgt tacaagcatt aacgtctgat aaaaaaggat taactcctga aaaattatta
     1081 gtcttgcagg atcatgtgct taattataat gtcgaggttt cattggtggg aacattagca
     1141 agaaaaattg tcgctgcagt tgagacgttg acccgctcat gaaatatata tcattattat
     1201 tgtttatcct gctgttgtgc ggctgcaagc agcaggaatt actgaaccat cttgatcaac
     1261 agcaagctaa tgatgtcctt gctgttttac aaagacataa tattaatgct gaaaagaagg
     1321 atcaaggcaa aactggattc tcaatctatg ttgaaccaac ggattttgct tctgcggtgg
     1381 attggctaaa aatctacaat ctcccgggta agcctgatat tcaaatatcg cagatgttcc
     1441 ctgcagatgc actcgtttca tctccacgag cggaaaaagc aaggctatat tctgctattg
     1501 agcagcggtt agagcaatct ctcaaaatta tggatggtat tgtttcttcg agagtacatg
     1561 ttagttacga tgttgatact ggcgatagtg gtaaaactgc tttaccaatt catatttctg
     1621 tacttgcagt atatgaaaaa gatatcaatc cagaaataaa aataaatgac ataaagagat
     1681 ttattgttaa tagttttgca agcgtacaat atgagaatat ttctgttgta ttgtcaaaac
     1741 gaagagacat tattgagcaa gcaccaacat atgaaattag cgaacctgtt tttgcttatg
     1801 ataaggctat gccggtttct atcttacttg cattaatatc cgtagcgact tgctggctgt
     1861 tatggaagta tagggccatt ctaactaatt tagtcaggtt gaaaattaag tagacatatg
     1921 agaacgttat gaatcttgcc ctaagaaaga ttatatatgc tccaattagt tatattcatc
     1981 ctcaacgagt aagcttgaat aatacaccta ttaacaatcc ggtgctgaga tctattacca
     2041 atgaaatgat tctcctgcag tataatcttt cagt
//
LOCUS       DAZGXS010000306         2068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGXS010000306 DAZGXS010000000
VERSION     DAZGXS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1443
                     /locus_tag="ACHKG5_004971"
     CDS             <1..1443
                     /locus_tag="ACHKG5_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP0870877.1"
                     /translation="KSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAETYRDEFDR
                     FIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVIVVGDRK
                     QLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCHPKIIQFC
                     NKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYI
                     APYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNP
                     ELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLL
                     YSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLL
                     ERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSIL
                     KKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            1944..>2068
                     /locus_tag="ACHKG5_004972"
     CDS             1944..>2068
                     /locus_tag="ACHKG5_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0870878.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGG"
BASE COUNT          595 a          368 c          479 g          626 t
ORIGIN      
        1 aaatctgatt ttaaaatttt actggggcgc ctgacatcat ggtctatgct ttatttaaag
       61 caacatctgc gaagaaatgt ttcaacaaga agttcattct cggcagagac atacagggat
      121 gaatttgacc gttttataaa gcgttttccg attattggta gcagtacgca ttcgattatt
      181 aactctatcg gcaagggggc ccttcttgat tatgttatta ttgatgaagc ctcgcagcag
      241 gatattgttc cgggcattct gggactggga tgtgcccgta atgtaatcgt tgtaggggat
      301 cgtaagcaac ttccacatgt tcctgtatta ctcccgaaca gcccatctcc accggcagaa
      361 tactataatt gtgagaaata tagtctactt gattcggttt gtatgttgtt caggaacatg
      421 gtgccagtaa cccttcttaa agagcattat cgttgtcatc ctaaaatcat tcaattttgt
      481 aataagcagt tttatgataa tgctctgata ccgttaacgg ttgattccgg tgaagcctca
      541 ttatctctgg tcatcacggc aaaaggaaac catacccgta atttttcaaa tctcagggag
      601 cttgaatctc tggaaggtca ttactgggat gaagaaagta gcaggggata cattgcccca
      661 tataatgcac aggttaatct ggctgaaaaa gtgctgcctg ctgattttgt gaaatcgacg
      721 gtgcataaat ttcagggacg ggagtgcgat gaaattgtgt tttccactgt gctggataag
      781 aagcgcagca gccagcacag tcggaacatc gcgtttgttg ataatcctga actggtgaat
      841 gtcgctgttt caagggcccg aaataaattt acgctggtga ctggtaatga tgtatttgaa
      901 cgccacgctg gacatattgc ggcgctgatt cgttacatca aatactacgc tgatgacgga
      961 gagattttcg agagcccggt catatcagca tttgatttgc tgtacagtga gtatgacaaa
     1021 tcactggaac gactgaatag tagattgaat tcaaatgatt cacactttaa gtcagaacag
     1081 attgtggcct gcctgttgcg ggatatcctg tcgcaggata gttatcggag tatgatgttt
     1141 cattcacaaa tagcgctgaa tcagttggtg ttgctggagc ggggggattt tacacaccgt
     1201 gaacagttat tcatgcgtaa ccgggccagt tgtgactttg ttgtttacta taaggtcggg
     1261 aagactccat taggggttat tgaagtggat ggaggttatc atctgaccag tgttcaggct
     1321 gaacgtgatg aacttaaaaa cagtatactg aagaaatgcg gcctgccatt gctaagatta
     1381 cgtactatag acagtgatat agaagggaag ttgggagcgt ttctgtcagg cctgacaggc
     1441 taaaatctga ataaaaaata ttgcagcggg aggtctcaaa cctgatttgg tcatgtgcca
     1501 cacacaattg tacgaacccc atggacacag gcttctttgt tttggttaca tcaacgtacg
     1561 tattcctgat acatataaaa ataagtgcta ttgctcaaag ctaacaggga atgtttctgt
     1621 tgaataattc cttagtgaat aaactttatc agggggtaat tctattacag tgaatacttc
     1681 ctgcaaaatg atatttgaat tgttcaccat ctgcatcata ttttcttatt tacaagttga
     1741 aaggatagta tatgttgaaa atgcatggat tttccaggtg tgttaccagt aagaagcagg
     1801 tggataaagg atgtgataac aaatccaaca accaggttaa tatctgaaga tttttgttgt
     1861 tattatttat ccccaaagcg gtgagttatt aatgaagttt gcctgaatgt ataaacagaa
     1921 ttattcgaca taaagccagg aaaatgagtg atacgctttt aaccgaaaaa atccttaccg
     1981 gagaaaatgt tttgcgtgct gcaatcgccc gcattgagtg gatttttgaa acatttccgt
     2041 cggtatgtct gtctttttca ggtggaaa
//
LOCUS       DAZGXS010000307         2034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGXS010000307 DAZGXS010000000
VERSION     DAZGXS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            83..430
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004973"
     CDS             83..430
                     /gene="tnpB"
                     /locus_tag="ACHKG5_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0870879.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            480..2018
                     /locus_tag="ACHKG5_004974"
     CDS             480..2018
                     /locus_tag="ACHKG5_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP0870880.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          453 a          565 c          613 g          403 t
ORIGIN      
        1 taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc
       61 tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt
      121 tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag tacaaacggc
      181 gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca
      241 ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg
      301 tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct
      361 ggcgatgctg ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac
      421 catgctgtaa atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta
      481 tgaacgacat ctcttctgac gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag
      541 cgctgatcca cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc
      601 aggcgcagct ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc
      661 gccgtatcgc acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga
      721 ctggtagggt gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc
      781 cgttccctga atcactaccc cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc
      841 cgaactgcgg cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga
      901 tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg
      961 atgccatcgt gcaggcacct gcaccttcgc ggcccatcga gcggggtatc gccggaccgg
     1021 ggctgctggc ccgcgtgctg acctcgaagt atgcagagca caccccgctg tatcgccagt
     1081 cagaaatata cggccggcaa ggtgtggagc tgaggcgttc actgctgtcg ggctgggtgg
     1141 atgcatgctg ccggctgctg tctccgctgg aagaggcgct tcatggctat gtcatgactg
     1201 acggcaaact ccatgccgat gataccccgg tccaggtact gctgccgggt aataagaaga
     1261 cgaagaccgg gcggttgtgg gcgtatgttc gtgatgaccg caatgcaggg tcagcgttgg
     1321 cacctgcagt gtggttcgct tacagcccgg acagaaaagg catccatccg cagactcatc
     1381 ttgcctgctt cagcggtgtg ctgcaagcgg atgcgtacgc cgggttcaac gagctgtatc
     1441 gcaatggtgg gataacggaa gctgcctgct gggctcatgc ccgccgaaag atccacgatg
     1501 tgcacgtccg catcccgtca gcactgacgg aagaagccct ggagcagatc ggtcagttgt
     1561 acgccataga ggcggatata aggggaatgc cggcagagca gcggcttgct gaacgtcagc
     1621 gaaaaacgaa accgttgttg aaatccctgg aaagctggtt gcgtgaaaag atgaagaccc
     1681 tgtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc acttaaccag tggccggcac
     1741 tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa caacatcgct gaaaatgccc
     1801 tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt cggctctgat catggtggtg
     1861 agcggggagc gctactgtac agcctgatcg ggacgtgcaa actgaatgac gtggatccag
     1921 aaagctacct tcgccatgtg cttggcgtca tagcagactg gccggtcaac cgggtcagcg
     1981 aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc aata
//
LOCUS       DAZGXS010000308         2022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGXS010000308 DAZGXS010000000
VERSION     DAZGXS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1560
                     /locus_tag="ACHKG5_004975"
     CDS             <1..1560
                     /locus_tag="ACHKG5_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0870881.1"
                     /translation="KWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEI
                     TQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     AGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGITIS"
     gene            1628..>2022
                     /locus_tag="ACHKG5_004976"
     CDS             1628..>2022
                     /locus_tag="ACHKG5_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0870882.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYL"
BASE COUNT          499 a          482 c          585 g          456 t
ORIGIN      
        1 aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagtggt
       61 aagggaacgg atgccagact ggtgaccacc gccatcaccg cagacacgga gcaccgtttc
      121 agcggcctgc cgctcgggga atacaccctg acggtgcggg cgataaacag ctatggccag
      181 cagggtgaac ctgccaccac caccttccgg attgccgcac cggcagcacc gtcgcggatt
      241 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg
      301 acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag gcaggttgaa
      361 accgcagccc gctatcttgg ctcggcgctg tactggatag ctgccagtat caatatcaaa
      421 ccgggccatg attattattt ttatatccgc agtgtgaata ctgttggcaa atcggcattc
      481 gtggaggctg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaagga
      541 gaaatcggga aaacacatct ggcccaggag ctgtggacgc agattgataa cggtcagctt
      601 gcgccggacc tggctgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcag
      661 accgtcaata aaaaactgga aaatcagagt gcggcaatcc agcagataca gaaagttcag
      721 gttgatacaa ataataacct gaacagcatg tgggccgtga aactgcagca gatgcaggac
      781 ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc aggaatgcag
      841 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca
      901 aagccgatgt ttgttggtca gggcgatcag atatttatga atgaagtgtt cctgaaatat
      961 ctgacggctc ccaccattac cagcggcggt aatcctccgg cattttccct gacaccggac
     1021 gggcggctga cggcgaaaaa tgccgatatc agcggtaacg tgaatgcgaa ctccgggacg
     1081 ctcaacaacg tcacgattaa cgagaactgt cgggttctgg gaaaattgtc cgcgaaccag
     1141 attgaaggcg atctcgttaa aacagtgggc aaagctttcc cccgggactc ccgtgcaccg
     1201 gagcggtggc catcaggaac cattaccgtc agggtttatg acgatcagcc gtttgaccgg
     1261 cagattgtta ttccggcggt ggcattcagc ggcgctaaac atgagaaaga gcatactgat
     1321 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     1381 gcgctggata atacgctgat ttacagtggt gttattgata tgcctgccgg tcacggtcac
     1441 atgacactgg agttttcggt gtcagcatgg ctggtaaata actggtatcc cacagcaagt
     1501 atcagcgatt tgctggttgt ggtgatgaag aaagccactg caggcatcac gattagctga
     1561 attttataac ccagatacgg gcgccagaaa tggcgccttt tttattgcag aaaagcgaga
     1621 ggtaattatg cgtaaattat gtgctgttat tttgtccgca gtagtctggc aggtcgccgc
     1681 tgctacgcca gcgagtgcag cagaacatca gtccacgctg agcgcggggt atctccatgc
     1741 ctcgacgaac gttcccggta gtgatgatct gaacgggatt aacgtgaaat accgttatga
     1801 gtttatggac gcgctggggc tgattacgtc cttcagttat gccaatgctg aggatgagca
     1861 aaaaacgcgc tacagcgata cccgctggca tgaagattcc gtgcgtaacc gctggttcag
     1921 cgtgatggcg gggccgtctg tacgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
     1981 tgtggcttac agccgtgtgt cgactttcta cggggattat ct
//
LOCUS       DAZGXS010000309         2021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGXS010000309 DAZGXS010000000
VERSION     DAZGXS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(505..1056)
                     /locus_tag="ACHKG5_004977"
                     /pseudo
     CDS             complement(505..1056)
                     /locus_tag="ACHKG5_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(1125..>2021)
                     /locus_tag="ACHKG5_004978"
     CDS             complement(1125..>2021)
                     /locus_tag="ACHKG5_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0870883.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          521 a          505 c          349 g          646 t
ORIGIN      
        1 ttttaaaaaa taacaaaaaa cagaacacta tcaaaacata aaaagcaaac aaaaaacaat
       61 aagcatgttg attgaatatt ttaaatacat gttcttttac aataaaaaaa tgaatggtta
      121 tcgaacaagc cagcaataat aaccttatta ttttcataag ctccatccac taaaatgctg
      181 gagatataaa aaatgaacca aacggcttct ttggcccata cttaggtttg gggtgtagaa
      241 tcttagtcat aagatctatg ccgagtcatg tgctggccac atacttaatg atacaaaaag
      301 ctatattttc atcttttctc ttgcgacttt tgatgcatct gccaagtaca tcgtaatgat
      361 aaccggcgac gaagatgcca gttttggttt caacttgcgc tttgatgagc ttgttgcagt
      421 cgtcgtaaac gtaattttgt gtgtaggtgt tgtagcgact ggtaaagttt ttctactctc
      481 catttttctg ttacctgttt aaagctactt ttgttctttc gttaattctt caattagtcg
      541 atctgcatca agcggctgtt tactcgcttt gagtttatat gcccattaca atgcttcttt
      601 tttatttccc tggtttttat aacaattcac caagaaccat attgctgaac tgtcattatt
      661 actggcagcc aatttaattt tttgtaacca ttcattagct tccttggttt caccttgttc
      721 ttgtaactct acatataacc gatactgagc tgtcgcatga ccattattag ccgctatttt
      781 taaccactga ctactttttt cttcatcata attggacaac ttgtagtaca agaacaacct
      841 aaaacccgcc tcgccatcgc ctcgttttgc ctggtgtgat aaagtattta cttcttctgg
      901 ctgcaaataa aagacgctta ctgttgattt tggttcacct tcaccttcac cttcaccttc
      961 accttcacct tcaccttcac cttcaccttc accttttcta gcagaaagct ccgccaataa
     1021 tttttccgcc tcatcagaat gttttgagtt atccatagca gccctcactc caaagttata
     1081 aagacatacc atgataatca acaaaaaagt aaatactttc attactattt gcttcctgta
     1141 ggtgtaaact tatgaaagtc ccctggttgt ttctcgtctg gcaccaattc aaacaccccg
     1201 ttgtccttac cgcctattgt tatcctagct gtttgcccca taatagcata gtttccatcc
     1261 ggccaaatcc atattatttc atcaggagca atcactgtag catcaagttc gttagctagt
     1321 ttctgtgcaa atgaattttt gccttttcct gtttcgcagg aaaacaagac tacaggcata
     1381 ccttcatgat atttaggatt cgctcttatg tcgctagcta attgttttat tgtcagatca
     1441 cttccttttt tgattctgtc atcactcata gatgtaggcg aaccatgacc tccaactgta
     1501 aaacccgttg gaaattccct tacattaaga gaacctttat atataggatc tgacggagaa
     1561 taataattca aatcaagtgc taaccccaac ggatctatat gttcaatagg attgagtggg
     1621 tactgataca ggttccatcc cccctccagc cctatcgggt cctgggtgat atatctcccc
     1681 tgcaacggat catagtaccg gttgcggttg tagtacagcc ccgactcctc atcatactgc
     1741 tgccccggca gacggagtga ctgttgaagg tgctgcgcgc tggtttcgcc cagcaggttt
     1801 ccccactcgt catactcccc ctgccacgcc gtttcccctt ccgggctgat gagcgccagc
     1861 ggcagtcccc ggtggtcgca gtggtaaaga tgaagtttcc tctccgggat gtattccgcc
     1921 tccagctgcg cggccatctg ctccgccgtc agcccgcact gcgcaagcca ctgctggctt
     1981 tcttcactca cgctgccctg ccgcagctcc cgctccagcc t
//
LOCUS       DAZGXS010000310         2012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGXS010000310 DAZGXS010000000
VERSION     DAZGXS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..863)
                     /gene="nleH1-2"
                     /locus_tag="ACHKG5_004979"
     CDS             complement(<1..863)
                     /gene="nleH1-2"
                     /locus_tag="ACHKG5_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP0870884.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVLYDRMRNEFNPIDISSYNVSD
                     ISWSEHQVMQS"
     gene            complement(970..1041)
                     /locus_tag="ACHKG5_004980"
                     /pseudo
     CDS             complement(970..1041)
                     /locus_tag="ACHKG5_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            1035..2012
                     /locus_tag="ACHKG5_004981"
                     /pseudo
     CDS             1035..2012
                     /locus_tag="ACHKG5_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          607 a          474 c          408 g          523 t
ORIGIN      
        1 taagattgca tgacttgatg ttcactccat gaaatatcag aaacattata agatgatata
       61 tctattggat taaattcatt tctcatacga tcatataaaa catttgtttc tgttgtatca
      121 acaaaaagaa ttcctttttt ctccagtctg tcaaacatat cgtaaatagc ctgttcagct
      181 tgtgctggta atgatggaat atccaaaaga gattccccat ttattttatt cattctaata
      241 ccaataacat ttccattatc attatatatt ttctctgcac tcccggaacc ataatactga
      301 ttgaaacaac gaacttcgct tgtcacctct tcatggcttt gagatatagt aaacatcttc
      361 aacacttttg ttgtatcttc catatcttca tacacgacag cattaccacc tttaccaata
      421 acattacctg gcacgggtga tttattgttg ctaaccctcg gcaactctga ttgagtataa
      481 tccgctggag gtaactccgg ctgcgcataa tccactggag gtaaatcagg tctgttcgaa
      541 tgaacagagc ctctttctat agtcgtgctc actggcgatg tattcagcat agcctcaatt
      601 tttctgctaa tctctccctt aggccatccc aacctgtgca acagattagt aaaacaacca
      661 ctatgacttt ctcttgtaac gcaaaactta ttatcagtga ccacaacacg atatgttctg
      721 ttgccaaccg ttacttgcgt cccgctatca gagtgaacag cagcatccct tacagaggat
      781 aaaacacgat tatcaggcga agtcaggttt ctggttaaag aattccatga acatcccaaa
      841 tttatagaag agggcgataa catacatttc aaccttcaaa ataaacctat ctaattattc
      901 ctaacagaca tcccccatac atgacaacaa aaaccggagc cggactccgg tttttgtgaa
      961 gctatcgggt tacttcattt cgccaatatt ttcccacttc ccgtcagcac gcaggatttg
     1021 cagcggtctt actacacact gacccgagga cgaaaaaaga gttcgggctg ggaagagaca
     1081 gaagagtagc aatttcagaa gcattcacac caacattgag cttttcagcg aaagtgggca
     1141 cgaatcattg ctggacaggg ttaaaggcac tgactcaatc actcttcata cgtggcttga
     1201 tcgatatgaa acaatcctca gctagagggg gagcaagccg aaaacattac tcgactacgc
     1261 cagcaaaatc agggcaattc gaagaaaatt gccggacaaa ccgctcactg acatatcaac
     1321 gaaagaggtg gcagcaatgc taaacaccta cgtcgcagaa ggtaaagcgg tttccgcaag
     1381 agtaatcagg tcaacccttg ttgacgtttt tcgaggggca atagccgagg ggcatgtggc
     1441 aacgaatcca gtaacaacaa cccgtgcagc aaagtcagaa gtaaggcgct caaggctgac
     1501 agctaatgag tatgtcgcta tttaccatgc tgctgagcac ctccccatct ggctgaggct
     1561 gtcaatggat ttagctgtcg ttacagggca gagagtgggc gatttgtgca gaatgaagtg
     1621 gtcagacata aacgatggtc atcttcacat tggacagagt aaaacaggag ccaaaattgc
     1681 cattccgctg gctctaacca ttgacgcact cgacatctca ctggttgata cactacagaa
     1741 atgcagggag gccagcagca gtgaaacaat aatcgcatca acctatcacg aaccactttc
     1801 tccagccaca gtatcacggt atttaacaaa ggcgcgaaat gcatccggga tctcgtttga
     1861 tggagaccca ccgacatttc atgaactacg tagtctgtcc gcgaggctat atcggaacca
     1921 gattggctac aagtttgcac aacgtcttct tggacataaa tctgattcaa tggcggcgca
     1981 ttatagggac agtcgcgggc gggagtggga ca
//
LOCUS       DAZGXS010000311         1971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGXS010000311 DAZGXS010000000
VERSION     DAZGXS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..132
                     /locus_tag="ACHKG5_004982"
     CDS             <1..132
                     /locus_tag="ACHKG5_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0870885.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..784
                     /gene="traT"
                     /locus_tag="ACHKG5_004983"
     CDS             83..784
                     /gene="traT"
                     /locus_tag="ACHKG5_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFP0870886.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(832..1083)
                     /locus_tag="ACHKG5_004984"
     CDS             complement(832..1083)
                     /locus_tag="ACHKG5_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0870887.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1107..1397)
                     /locus_tag="ACHKG5_004985"
     CDS             complement(1107..1397)
                     /locus_tag="ACHKG5_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0870888.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          596 a          464 c          415 g          496 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
      661 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
      721 gtttgaagaa gctaaacctc atctggaaga ccagctggca aaatcaatcg ccaatatact
      781 ctgatataaa tatcccggtg gccattcacc tggcctccgg ccctcttagt ctcaacgttc
      841 agacaattca gatattattt tccctgtcac accatatacc catgccggaa cagaatctaa
      901 tgacggtacc agtaaattct tcagcgttcg gtttgcctga cgcccgtagc tgtatttgtg
      961 tctgtttctt cttatacaag agcagtagta caatttctct tccagagtca gttcaacttt
     1021 aggacgtaac caataaagat ctcttccact ataaaatggt gaatcctgta caaacgattc
     1081 cataacagaa cctcctgctg ctacagttaa cacagctgct ggcatggact aataccatct
     1141 tttaatttaa ccctggcgga gacgccatta tttttggacg ttcttgaaac aaaattaatt
     1201 cctgaagaat ctttctccag tttattcagc tcaagattca gtccataatt aacgtcaaat
     1261 aactctgata aaggtatcag tttatgttca ggataatgat tatcccgacg acaacaaata
     1321 ttaaaatcct gcatattacc cgacatcagg agttcaaaac cgtccccggc atcattcata
     1381 ctggcagata aattcattac ccagtttagt gctaatcaac aggtacggct taacttacag
     1441 gaattccccg ggaggggcgg actcatcctc agttagcggc aaaatcgcat cacagccaat
     1501 cgcctgtctc gattccccat cggcgaacaa tgtatgtggc cacaaaatcc tggctggctc
     1561 agaacgaaga gcatctgagc aaagaggtca tcaatctggt atgaatatct ggcagaaaat
     1621 atcccctgaa tccacaactg ctgtgcatat ctgctcaaat cagcatcaat cataaaccag
     1681 taatcacagt gcctgtgatt gggttacagg aataagtccc ccttcataag gcatatcatc
     1741 aataccggtg aaggaaaaga aagcatcaga tggcctgata gtctgcgtca gtcaatgaca
     1801 tcagtgcttt atccggaaca atgtatccat gttggcgaca gagaaagcca ttgccatgaa
     1861 cagttttgtt tatccccgga aagtcatttc tgttacaaat ttatattgac aaacttgctt
     1921 tcgatgagaa acatactatt tcaatgaaat gcagaatgag gcaaaatttc g
//
LOCUS       DAZGXS010000312         1922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGXS010000312 DAZGXS010000000
VERSION     DAZGXS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1922
                     /locus_tag="ACHKG5_004986"
     CDS             <1..>1922
                     /locus_tag="ACHKG5_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFP0870889.1"
                     /translation="DFLGIQDNTIKVKLESDIKPISEIQQPLHSILSRQKEHVKNLLS
                     GLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGRLYRVDIDTRVI
                     GLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAIDHAGAVSDIKNIV
                     DKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTAGRYLSNVAKVIKL
                     PLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAI
                     VPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKATGIIDLSNNQV
                     LGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFACTKLSDAGEPD
                     IFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYGETLRANTEV
                     TLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLH
                     MIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSD
                     GRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTFSLGSGGGVI
                     YSGGGSNTYFVPATLQ"
BASE COUNT          651 a          288 c          388 g          595 t
ORIGIN      
        1 gattttttag gtattcagga taacacaata aaagttaagc ttgaaagtga tattaaacct
       61 ataagtgaaa tccaacagcc ccttcattcc attctttcac ggcagaaaga acacgtaaag
      121 aatttactat ctggattgct tgatgagttt tcaaataagt taagaaagca aggtctttca
      181 cttaaaacaa atgttctttc agttaataac ttcaaagaat caaagataaa tagtgataca
      241 gtggaggtta cagtaacaga tctgcaagga agactttatc gtgttgatat agatacaaga
      301 gttattggct taacttttaa agaggggata aatagtttat ccgaagcact cgaacacatg
      361 aatatcgatg cgattatgtc tgttattggc ttggttcaat atgcacgaat gataaaaatg
      421 aatgacaata ttagtgcaat agatcatgca ggagcagtat cagatataaa aaatattgta
      481 gacaaatttt taggcggaat tttgactctt actaataatc gcgtatataa tcctggcggt
      541 gtatctggtg cctcgcttga aggatttata tcttctgggt tggaggtgtg tgcttcaaga
      601 atgggtggca ccgcagggcg ttatcttagc aatgtggcaa aagtaattaa attacctttg
      661 ctagatatag gcataaatat ttggtcatta tatgattctt cattaaatca tgctaaagcc
      721 accacacaaa ttgaatatat atcaacagca atagatgtat ctttttcatc tataaataca
      781 gctttgtcaa taggcgcaat cgcatatcct cctttggcaa tagctatagt tccgataact
      841 attttttctc atgaagtaaa aaactatgct gtttatgtga atcaaattaa tgagcgccat
      901 aagttatggc tcgaagctga aaagtatctg gacaatggaa gtgcaaaggt tctaagcatt
      961 aacaaagcca caggcataat cgatttatca aataatcagg tgttaggtaa tatttattta
     1021 gatatgagag aaaatcctcc aatattacac ggtgagaaat catataatag cggaaagaat
     1081 attggtagtc atcctgatat gactgacaga gagattatgg agtctagagc atacaatttt
     1141 gcatgcacaa aactctcaga tgcaggtgaa cccgatattt tcggttgggg agataaggaa
     1201 atatgtaatt caatggaatt atcagaatca caattagcta atgggtattc taaccgccaa
     1261 tggccttctc agattccagt tattccagaa ggaatttata atacagtata tcttggatat
     1321 ggagaaacat tgcgagctaa tactgaagtt acattgagta gcactggtta cttttacgag
     1381 attgcaaggg catatactga tgatgaacta tctgagccat tattaacagt atgtaaccaa
     1441 catagtcatg ttattggtgg aaaagaacca ttaacaatta ttattcccgc tattgaacat
     1501 ggtatgcttg gctctaatct acatatgatt gaacgtttta aaaactataa tttctcgata
     1561 tcaggaggga agggagggat aaaattaatg gttggtggta ttggtgatta caacatagaa
     1621 tgtacccccg gtgttagaaa tattatatcg tttgaacaat tatcacgtga cttcaatctg
     1681 gatttggatt tatcagatgg gaggaaacaa aattttaatt ttcatcatcc ctcaggtttt
     1741 tattcaggaa aagttatgag cataacgcaa aagggtataa atgcggttgt tggtactaaa
     1801 ctgggatatg ataagattcg tggtaataat cttgataata ccttttctct tgggagtgga
     1861 ggaggtgtta tatattctgg aggagggagc aatacctatt tcgttcccgc cacactacag
     1921 ga
//
LOCUS       DAZGXS010000313         1913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGXS010000313 DAZGXS010000000
VERSION     DAZGXS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(13..378)
                     /locus_tag="ACHKG5_004987"
     CDS             complement(13..378)
                     /locus_tag="ACHKG5_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0870890.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            420..605
                     /locus_tag="ACHKG5_004988"
     CDS             420..605
                     /locus_tag="ACHKG5_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0870891.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(735..875)
                     /locus_tag="ACHKG5_004989"
     CDS             complement(735..875)
                     /locus_tag="ACHKG5_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870892.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1232..1456)
                     /locus_tag="ACHKG5_004990"
     CDS             complement(1232..1456)
                     /locus_tag="ACHKG5_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870893.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1521..1727)
                     /locus_tag="ACHKG5_004991"
     CDS             complement(1521..1727)
                     /locus_tag="ACHKG5_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0870894.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(1731..>1913)
                     /locus_tag="ACHKG5_004992"
     CDS             complement(1731..>1913)
                     /locus_tag="ACHKG5_004992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870895.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
BASE COUNT          529 a          511 c          427 g          446 t
ORIGIN      
        1 ccccgggtct ggtcacaggc cagtgattcc gtcggccctg agcgtgccat caggaagctc
       61 aggcaacgtc ggatcaggca taccactcgc cgcttcactc gctgacttct ggcgatggca
      121 ttcagtacag agggtccaga ggttcgtctc ctcattacca ccaccgaact gaagtgcaat
      181 gcggtgatcg agttcactgt cacaaaggtc aaccacgcgt ctacagagac aacagtgtcc
      241 ggcatccctc cgccagatac gacgtttgag ggaaacccgg gcactgccac tgacacgacg
      301 ctgttccccc ctcaggacat ttatccgccg ggtgttcaga gttttgattc tgcccggtaa
      361 cgtacgaagc acagccatgt aaaatcctcg ccatatagct tgtcaccaga ggaaagaaaa
      421 tgtcatcgaa aaaccggacc cgcagaacca caatccgcaa tatccgtttc cccaatcaca
      481 tgattgaaca gatcaatatc gcccttgaac ataaaggttc cggtaacttt tcagcgtggg
      541 ttattgaagc ctgcagaaga aggctgtcaa cagagcgttc gggtatgaat tacataatta
      601 agtaacatgg tgttcacaga acacacagtt accggacaca tcagttttcc attcgtcccc
      661 cggcagtaca ggcttcccgt ctgacgggat agcctgaaaa aacacagaaa attatttgtt
      721 ataattaata taacttactc aaaaaaaagc gacgagaaaa tcagcatcaa cgagcaataa
      781 gcgccaatac gtgataacaa atggcagcca tatttatctg cagtataagc aatggacagg
      841 ataaccacac cagaaaccgt cagcataaaa tccatttgaa cttccccgga caaaatcgac
      901 tcatctaaag atttacagct ctttttatta tcaatatgtt aaaagtaaaa taaacagatg
      961 ttcaataata cgaatacaaa aacgtgctga aaatcaatga atccatttct gtgttatcaa
     1021 ttaatagtga taaacatccg gcttcttcca ccatcgcacc ggaccagcga ccatgagggg
     1081 acaacgccgc gctccgttaa cgcggtaaac cccggtgtgt atcgtttttg attatccccg
     1141 cacactcgcg cagaggattc tcccggtcgg gctgcggtct ctgttaatgc aggaatacgg
     1201 cgacaatacc gcgcatgaat aataaggtcg ctcaacacac tggctgtaat gcagcggata
     1261 ccatgcggca tttagcggca ttcatcgtac actcaacggt tagctcttca ttcgtggcat
     1321 tcacctgaaa ggtcctggag tgtaattgcg tacatttacc actgaacgaa ccttcaacaa
     1381 gaacacgacc acgctgcaaa atacggaacg gaattgttcc ctgaaaaggc tttacggtta
     1441 ccaataattt cttcatgcat tctccgaata acaaaaatac tagttaatac actgagtgcg
     1501 gatatattcc tgaagcattc tcaatgctgc ctggtcgctg atgattccgt ctctgatacc
     1561 gagaacgttt cgtccagcaa ccggagagag ttcgacggcg gcatcattgc ccacgccgga
     1621 ggtgccggtg gcttcacgca cggtaccggg gcaggtggcg ttgatccgca ggcgcttacg
     1681 accagcggca acatcagcac gcagagtttc attttcagct ctcgcatcgg ctaattccct
     1741 cgagtatttt gcatcgagcg cagcaacatc acgctggcgc acctgcatgt cagtaatggt
     1801 ggcattcgcc tgttccagct ctctggcttt tttatcgcgc tgcgctttgt aggtgagcgc
     1861 gttgtcacgg taatggtttg ttgccagcca cagcgcacca cagccaaccg cca
//
LOCUS       DAZGXS010000314         1897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGXS010000314 DAZGXS010000000
VERSION     DAZGXS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..83)
                     /locus_tag="ACHKG5_004993"
     CDS             complement(<1..83)
                     /locus_tag="ACHKG5_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870896.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVD"
     gene            complement(83..619)
                     /locus_tag="ACHKG5_004994"
                     /pseudo
     CDS             complement(83..619)
                     /locus_tag="ACHKG5_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            683..1330
                     /locus_tag="ACHKG5_004995"
     CDS             683..1330
                     /locus_tag="ACHKG5_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0870897.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          606 a          399 c          444 g          448 t
ORIGIN      
        1 ctgtctacga tattccccgt gtttaccggg ttgttaagga attctgcaag tttcaccggg
       61 ttatcgatga gctttatttc catcactgct ccgcaatgat tttgatggtt gtggcagtaa
      121 acgccgcgcc attcgactga atggttaacg tgctgccatt tgtggcaaga aagccgtctt
      181 tatccacgct gaagaacgta gctaacagga tgttgtcggt tgttgttgcc gagttgcgac
      241 tgcttactag cgtgtcagga acagaaccgg agaatgttag ctgcattgac ctgttggcgg
      301 ttccgctggg ccacgtcccg acaatcgaca gcttgaagaa caaggttttg ttctcgttga
      361 acacaaccat cttgttgtta acggtgtcga agaatggtgc caacgtcccg gatgacggcg
      421 taagcgtttt cagcaggcta acaaggttgg tcggcgctgt cgggatggtt actgatacgc
      481 cagagtaaac aacctctgac ttcttgcgcg tagtggcata ctccagagca ttaatgcgcg
      541 tttcatggtc tgaaacctgc gattccagcg actgaactct ggtatcaagc gatgcaatat
      601 cgctttcatt caaggcaatc agtgggttta cgtcatggag tgggctcaac accagtcaga
      661 gccgcatgta cacgcaataa tcatggtgaa cgaaagaagc attaatgggc aagctggcaa
      721 aattgtcagc aaatgggtaa cactggcaaa caatgcaatc ccatcaaatg ttcctcccca
      781 gtgggatcta gatttgaaag gtcagcaata ttcagagcct acaaagtgta taggtaaaca
      841 gattgattac atggcaaagc ccgaaactaa gttaaatgac tttaatagag aggcgagcaa
      901 gagcctatat gattggtcag atgccaacgt ttggggttat tcgaatggct gggcgcggca
      961 tgagataaaa aaagaaacac tttctatcac tggctttcat gctgtaagac gcatactaaa
     1021 agccgttcag gcatcaagga aagacaacta caaagctaag cgacagatac agcgtacatt
     1081 gaaacgacaa gacataaatc gatcaccaat caccccgttc caagcaccgt taattagcaa
     1141 acacgattgg gatttatcag tgatagcggt caccaaagag gcagagacag agcgcaggga
     1201 attactatcc tcacagataa taaactcgaa caaagtacaa acccacccac tgttaaggat
     1261 gttaagaaga gcacgagtga gagcatccag agccaaaaga gaaagcggag gttatgacca
     1321 ttactactag tagtgacaga tcgtgacaag gcggtgcaaa aaacgaccaa taataagaga
     1381 gatatgcctg ccaaaccgcc aaaagcgcgg agcgcggcaa acgccgcgag aggcgcaaga
     1441 aggcgaacct atccattgaa acggtcagaa gcgcatcgcg ctcgacgacc gaagtcccca
     1501 ctaacaccgt taacgaaaga tcaaagtctt agaaaaaaat gacgtttctt tgatggactt
     1561 ccatccgtaa aaatcaaatt ttgagaaaaa gtacttatag aaattaatcg ctctataagg
     1621 ataagttgac gttagtttaa agtgataaaa cattaaagaa aaaacgtaaa atcaaataca
     1681 taaaataagt caccaaggga tcattttcag aaaacaacgt caacgtaacg tcaactctgt
     1741 aagaactttt ttggtcactt tttgggttga atgtacgtaa aaatccccag cagtgaaatt
     1801 tgagcgcgta agcacaattc tgaggggctt tttgtctgta ggttaggcag tgtaactaac
     1861 aatacaagaa gttagctctg actaactatt taattaa
//
LOCUS       DAZGXS010000315         1889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGXS010000315 DAZGXS010000000
VERSION     DAZGXS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..177)
                     /locus_tag="ACHKG5_004996"
     CDS             complement(<1..177)
                     /locus_tag="ACHKG5_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP0870898.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFH"
     gene            complement(174..845)
                     /gene="bet"
                     /locus_tag="ACHKG5_004997"
                     /pseudo
     CDS             complement(174..845)
                     /gene="bet"
                     /locus_tag="ACHKG5_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            875..1057
                     /locus_tag="ACHKG5_004998"
     CDS             875..1057
                     /locus_tag="ACHKG5_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0870899.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            1054..1224
                     /locus_tag="ACHKG5_004999"
     CDS             1054..1224
                     /locus_tag="ACHKG5_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP0870900.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            1217..1837
                     /locus_tag="ACHKG5_005000"
     CDS             1217..1837
                     /locus_tag="ACHKG5_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0870901.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
BASE COUNT          525 a          458 c          494 g          412 t
ORIGIN      
        1 gtggaagtag gacattttca tgtcaggcca cttctttcct gagcggggct ttgctatcac
       61 gttgtgaact tctgaagcgg tgatgacgcc gagccgtaat ttgtgccatg catcatcccc
      121 ctgttcgaca gctctcacgt cgatcccggt acgctgcagg ataatgtccg gtgtcatgct
      181 gccaccttct gctcagtggc tttctgtttc aggaatccaa gagctttcac tgcttcggcc
      241 tgtgtcagtt ctgacgatgc gcgaatgtcg cggcgaaata tctgggaaca gagcggcaat
      301 aagtcgtcat cccatgtttt atccagggcg attagcagag tgttaatctc ctgcatggtt
      361 tcatcgttaa ccggagtgat gtcgcgttcc ggctggcgtt ctgcagtgta tgcagtattt
      421 tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa ggccagacgg
      481 gcacactgaa tcatagcttt atgccgtaac atccgtttgg gatgcgactg ccacggcccc
      541 gtgatttctc tgccttcgcg ggttttgaat ggttcgcggc ggcattcatc catccattcg
      601 gtaacgcaga tcggatgatt acggtccttg cggtaaatcc ggcatgtaca ggattcattg
      661 tcctgctcaa agtccatgcc atcaaactgc tggttttcat tgatgatgcg ggaccagcca
      721 tcaacgccca ccaccggaac gatgccgttc tgcttatcag ggaaggcgta aatttctttc
      781 gtccacggat taaggccgta ctggttggcg acgatcaaca atgcgatgaa ctgcgcatcg
      841 ctggcatgaa agcgcgatgg ggagacaggg cggcatgaga cgacagcgac gaagtttcac
      901 cgacatcatc tgcgaaaact gcaaatacct tccaacgaaa cgctccagaa ataaacgcaa
      961 gccaatccca aaagaatctg acgtaaaaac cttcaactac acggctcacc tgtgggatat
     1021 ccggtggcta agacattgtg cgaggaaaac aaggtgattg accaaaatcg aagttacgaa
     1081 caagaaagcg tcgagcgggc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     1141 gaagttcacg tgtgtgagca ctgctgcgca gagctgatga gcgattcgaa tagctcgatg
     1201 cacgaggaaa aagatgatgg ctaaaccagc gcgaagacga tgtaaaaacg atgaatgtcg
     1261 ggaatggttt caccctgcat tcgctaatca gtggtggtgc tctccagagt gtggaaccaa
     1321 gatagcactc gaacgacgaa gcaaagaacg cgaaaaagcg gaaaaggcag aaaaggcagc
     1381 agagaagaaa cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcaaaaact
     1441 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagccgtac acgccttcat
     1501 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
     1561 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
     1621 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
     1681 cgtcgaactg attagccgca tcgggcagga agcagtagag gaaatcgaat caaaccataa
     1741 ccgctatcgc tggactgtcg aagagtgcag ggccatcaag gcggagtatc aacagaaact
     1801 taaaaaactg cgaaacagca gaagtgaggt tgcatgaata tctacgaaag aattgatggc
     1861 agcaaatacc gaaatatttg ggtagttgg
//
LOCUS       DAZGXS010000316         1872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGXS010000316 DAZGXS010000000
VERSION     DAZGXS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..383
                     /locus_tag="ACHKG5_005001"
     CDS             <1..383
                     /locus_tag="ACHKG5_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP0870902.1"
                     /translation="DKYYIKPDAVYLGIYEGLVLAGVHEVRNFWHSVVECHAVYDPGF
                     RGEYALQGHRLFCKWLLENSPFLNSITMVPDTTKYGRAIIRLLGATRVGHLDDAYMSN
                     GKPVGITLYQLPRSKYEELLNVST"
     gene            370..1050
                     /locus_tag="ACHKG5_005002"
     CDS             370..1050
                     /locus_tag="ACHKG5_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP0870903.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            1060..>1872
                     /locus_tag="ACHKG5_005003"
     CDS             1060..>1872
                     /locus_tag="ACHKG5_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP0870904.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSIL"
BASE COUNT          507 a          431 c          481 g          453 t
ORIGIN      
        1 gagataaata ctacatcaag cctgatgcgg tatatctcgg catctacgaa ggattagtgc
       61 tggctggagt tcatgaagtg cgtaacttct ggcatagcgt tgttgaatgc catgcggtgt
      121 atgaccccgg attccgtggc gaatatgcac tacaagggca tcgattattc tgcaaatggc
      181 ttctcgaaaa ctcaccattc cttaacagca tcaccatggt tcctgacacc accaaatacg
      241 gacgggcaat tatccgtttg cttggcgcta cccgtgttgg tcaccttgat gatgcgtaca
      301 tgagtaacgg aaaaccggta ggaatcaccc tctatcaatt acctcgttcg aaatatgagg
      361 agctattaaa tgttagtact tagcgaaagc ttcaagaata aattgcttcc catgaatggg
      421 tatatgaaag gcggcagcga ctccggatct aaagcccagg cacgcgcaac tgaaaagggc
      481 atcgaactgc agcgtgaaat gtggcagacg aacatgcaaa accttgcacc gttcacgcca
      541 ctcgctcagc agtacgtatc acagttgcag aatctttcct ctcttcaggg gcaaggtcag
      601 gcgcttaacc agtattacaa ctctcagcag tataaagacc ttgcagggca ggcgcgctat
      661 cagagtctgg cagcagcaga ggcaacgggt ggattaggct ctacagcaac aggaaaccag
      721 ttagcagcaa tcgcacctac actcggtcaa aactggctgt caggtcagat gaacaactac
      781 aacaatctgg caaatatcgg ccttggtgct cttacaggtc aggcaaacgc cggacagaac
      841 tacgctaaca atgtcagcca attgtatcaa cagcaggcgg cagcatcggc agcaaatgcg
      901 aataagcctt caggcctaca gagttttgct acaggtgcca ttggtggggc cgcatcaggt
      961 gcaatgattg gtagtgcagt tcctgttatt gggactggta ttggtgctct tgctggcggt
     1021 gttatcggtg gtcttggatc attgttttaa ggtgggaata tggctacttg gcaacaagga
     1081 atcaactcag gcggttttct tgctggtatc ggtgggcaaa actcaaatgc gccaaaggca
     1141 agtgatgtaa gtgaggcgtt ggcctatatt cgccagaaca acgaaatgga gcgttcaggt
     1201 cgcaataaca tcggccttca ggcgttgcag ggacttggta gtgtcgctca aacatctcaa
     1261 gccgcaaagc aacaggaagc ggatgctgca ttccaaaaag aatatgcggc agccatccag
     1321 tccggcgatc gacagcaggt tcgagatctg atgaccaaat atcctggtca attagagaag
     1381 attcagtctg gtatgaagtg ggcagacgaa gaccagcgca attctatcgg caccttagcg
     1441 gctggcgcac gccttgcggc ctcgtctcca gaagcaatgc aatcatggct gcaaaacaac
     1501 gccaaggaac tggcgcgcgt cggtgttgac cctaacaacg ttgctcagat gtatcagcag
     1561 aatccttcag ggtttggtga gtttgtttat caccttggga tggctgctct cggtccgatt
     1621 gactacttca atgttcagga caagatggct ggtcgtgaga ttgaccgagg caggctggca
     1681 gagacaatcc gcagcaatca ggccggagat tttgagttta agtcaaagcc gtcatattta
     1741 gttctatcat ttgctgaaat atcatctgta gtctttatcc taatgtctgt ctatatggtg
     1801 gcaaaaagtc aaaatagcaa tggattacag tatgatgcgc tttttatacc atcaatggcc
     1861 ttttcaatat ta
//
LOCUS       DAZGXS010000317         1839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGXS010000317 DAZGXS010000000
VERSION     DAZGXS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..513
                     /locus_tag="ACHKG5_005004"
     CDS             <1..513
                     /locus_tag="ACHKG5_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP0870905.1"
                     /translation="NNYIYSESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVE
                     MLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWI
                     VLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDII
                     RQKERYKHED"
     gene            517..>1839
                     /gene="cpsG"
                     /locus_tag="ACHKG5_005005"
                     /gene_synonym="manB"
     CDS             517..>1839
                     /gene="cpsG"
                     /locus_tag="ACHKG5_005005"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0870906.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVK"
BASE COUNT          486 a          439 c          489 g          425 t
ORIGIN      
        1 aataattata tctactctga gtcagcgttg gtagccgcca ttggaattga agatatggtt
       61 atcgtgcaaa ctaaagatgc cgttcttgtg tctaaaaaga gtgatgtaca gcatgtaaaa
      121 aaaatagtcg aaatgcttaa attgcagcaa cgtacagagt atattagtca tcgtgaagtt
      181 ttccgaccat ggggaaaatt tgattcgatt gaccaaggtg agcgatacaa agtcaagaaa
      241 attattgtga aacctggtga ggggctttct ttaaggatgc atcaccatcg ttctgaacat
      301 tggatcgtgc tttctggtac agcaaaagta acccttggcg ataaaactaa actagtcacc
      361 gcaaatgaat cgatatacat tccccttggc gcagcgtata gtcttgagaa tccgggcata
      421 atccctctta atcttattga agtcagttca ggggattatt tgggagagga tgatattata
      481 agacagaaag aacgttacaa acatgaagat taacatatga aatctttaac ctgctttaaa
      541 gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatattgc ctggcgcatt
      601 gggcgtgcct atggcgaatt tctcaaaccg aaaaccattg ttttaggcgg tgatgtccgc
      661 ctcaccagcg aagcgttaaa actggcgctt gcgaaaggtt tacaggatgc gggcgtcgat
      721 gtgctggata tcggtatgtc cggcaccgaa gagatctatt tcgccacgtt ccatctcgga
      781 gtggatggcg gcatcgaagt taccgccagc cataacccga tggattacaa cggcatgaag
      841 ctggtgcgcg aaggggctcg cccgatcagc ggtgataccg gactgcgcga tgtccagcgt
      901 ctggcagaag ccaatgactt ccctcctgtc gatgaaacca aacgtggtcg ctatcagcaa
      961 atcaatctgc gtgacgctta cgttgatcac ctgttcggtt atatcaacgt caaaaacctc
     1021 acgccgctca agctggtgat caactccggg aacggcgcag cgggtccggt ggtggacgcc
     1081 attgaagccc gatttaaagc cctcggcgca ccggtggaat taatcaaagt acacaacacg
     1141 ccggacggca atttccccaa cggtattcct aacccgctgc tgccggaatg ccgcgacgac
     1201 acccgtaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga tggcgatttt
     1261 gaccgctgtt tcctgtttga cgaaaaaggg cagtttatcg agggctacta cattgtcggc
     1321 ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca cgatccacgt
     1381 ctctcctgga acaccgttga tgtggtgact gccgcaggcg gcaccccggt aatgtcgaaa
     1441 accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta cggtggcgaa
     1501 atgagcgctc accattactt ccgtgatttc gcttactgcg acagcggcat gatcccgtgg
     1561 ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaaat ggtgcgcgac
     1621 cggatggcgg cgtttccggc aagcggtgag atcaacagca aactggcgca acccgttgag
     1681 gcaattaatc gcgtggaaca gcattttagc cgcgaggcgc tggcggtgga tcgcaccgat
     1741 ggcatcagca tgacctttgc cgactggcgc tttaacctgc gctcctccaa caccgaaccg
     1801 gtggtgcggt tgaatgtgga atcacgcggt gatgtaaag
//
LOCUS       DAZGXS010000318         1830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGXS010000318 DAZGXS010000000
VERSION     DAZGXS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..97)
                     /locus_tag="ACHKG5_005006"
     CDS             complement(<1..97)
                     /locus_tag="ACHKG5_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870907.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSD"
     gene            complement(81..>1830)
                     /locus_tag="ACHKG5_005007"
     CDS             complement(81..>1830)
                     /locus_tag="ACHKG5_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0870908.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          347 a          566 c          448 g          469 t
ORIGIN      
        1 tgtcagaatt tgaattgtta tcaaacccaa ggtaaacaac aaataaacaa agggtaatta
       61 aaaaaatgac aagtttgcct ctaataattt gaagcatctg aactcccttg ctttgctttt
      121 attggattat ctgcttttat accatctaac ccgaagagtt gcccaatgtt atatttttca
      181 atacatgcat cgctacattt ggcagaaaaa tcgccacaag caataccgac agtgttattt
      241 ttaagatcca tgattctttc tttagctggc tgaccttgtc tatccccagc cgcctcatga
      301 tttttaccta tcacgtcagc tattgttgat ccaaatgtct tagtcattcg acacatcaag
      361 taacaatatc tcattgcatc ggcggcccca ttatgagctc cgccaacaat agcatccata
      421 tcataaccga actggtaagc actgttagta atgctgtttg caacaatcaa tccaggtaca
      481 ccagatacgg cataaattag tccacctgat gtcatattct caatcgcatc taaccccatt
      541 gggtctatca cttgtatggg attcaacgga tactgataca tattccatcc acctctcaac
      601 ccaatcgggt ccggggtgat ataccgcccc tgcaacggat cgtagtaccg gtgacggtta
      661 tagtacagcc ccgactcctc atcatgctgc tggcccggca gacggtacgg ctggtgcagg
      721 tgatgcgggt tctcctcatt aagctggttg ccccattcat catattccgc gctccacgcc
      781 gtattgccgt cttcgctgat aagcgccagc ggcagtcccc ggtggtcaca gtggtacaga
      841 tgcagcgtgc gcgccggcgt gtattccggc tccacctgtt tttccagttg ctccaccgtc
      901 agtccgcact gcgcaagcca cgcccggctt tcactgctca cgcggtctgc ccggatttct
      961 tcctccagcc tgtccagcat cctcaccagt tctgccggga acaccacacc gtgaccgtcc
     1021 tcactccctt cctgctggag tttctccgcc aggctgcggc gctgcgcttt ctcccgctcg
     1081 ccgttctccg tttcgatgcg gatgagcggt gtgaagctcc ccggccggta taccgtctgg
     1141 atacgtgtgg tgccggtctg taccgtcgtc agcctgtcgc cgtcccaccc gtaccaggtc
     1201 acctccggtt tacgcgacag cgacatccac cccgtcaggt cacgctcccg ccgccacacc
     1261 cgtttccccg tccggcggcc cagcgggtca tagaggtagc ggctctcgac cagcggctcg
     1321 ccgtattgta tccgcgtgta gaacaccagg cggtgctggt tgtcgtaatg gtagtggtgg
     1381 gtccgctcgt cgtgcatccg gataacccct tccggaatgc ggtccgtctt ctccgtcagc
     1441 ctgccgtatt catcgtagcg gtagacatag tgcgcatctt ccgcgatgcg gttatccggc
     1501 cacgccgtga gcgtgctgtc cggatgcagt tccgggtccg gcagccggtt tcctgccggg
     1561 tccgttgcat acgggatatc gatatccagg ttcgctgccg tggtgtggac gcccgtcagc
     1621 cttcccgtgt cgctgtaacg gtactcccgg ctctcctgcg ggccgctgat gcggattagc
     1681 tgtccgttgt cgttccagtc gtagtcacag tcaagctgcg ggaggttcag gtgcctgctc
     1741 tgtagctgcc ctgtgagggt gtacgccgtg acctgttcgt accccgctgt actgcccgcc
     1801 ccgccgaagc tgcgggccgt ctcacggtgc
//
LOCUS       DAZGXS010000319         1828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGXS010000319 DAZGXS010000000
VERSION     DAZGXS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            58..357
                     /gene="espZ"
                     /locus_tag="ACHKG5_005008"
                     /gene_synonym="sepZ"
     CDS             58..357
                     /gene="espZ"
                     /locus_tag="ACHKG5_005008"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFP0870909.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
     gene            441..818
                     /gene="sctI"
                     /locus_tag="ACHKG5_005009"
     CDS             441..818
                     /gene="sctI"
                     /locus_tag="ACHKG5_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP0870910.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            821..1393
                     /gene="escJ"
                     /locus_tag="ACHKG5_005010"
     CDS             821..1393
                     /gene="escJ"
                     /locus_tag="ACHKG5_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP0870911.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1399..>1828
                     /gene="sepD"
                     /locus_tag="ACHKG5_005011"
     CDS             1399..>1828
                     /gene="sepD"
                     /locus_tag="ACHKG5_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP0870912.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHL"
BASE COUNT          613 a          360 c          360 g          495 t
ORIGIN      
        1 taatttggtt atttataacc aggtaaatgt ctaaattaga caaaaggaga aagatcaatg
       61 gaagcagcaa atttaagtcc ttctggtgca gtaatgccgc tggcgacctc actcagtgga
      121 aataactcag tggatgagaa gacaggagtg attaaaccag aaaatggaac aaatcgcacc
      181 gttagagtta tagccggatt agcacttacc actacggctc tggcagctct aggtacaggt
      241 attgcagcgg catgctcgga gacgagcagc acagaatact tagccctggg tattacttct
      301 ggcgtactag gtactcttac tgcggttggc ggtgcattag cgatgaaata tgcctaaccg
      361 gcccatcgca taaataaaag atagaggtaa tggatgtatt atgcccttgc ctctttcata
      421 aaaagaggct aacagtaaat atgaacaaca ttaatcaatc ggaaaacatt aatattcaat
      481 taaataaagc gccccaaaca aatttcgttg atgagcatac ctcgttagcc tcagccccct
      541 ctgcggcagg tgcggctcag tttcttgatc aattactccc taaaacagcg ggagtgtctt
      601 ctccagaaca agtgttgatt gaagaaatta aaaagagaca tcttgcaaca atgaacagcg
      661 atctcagttt cgatgctcta tctgcaggtg ggctctcgcc agaagacgtg ctcaccttac
      721 aaaagaatgt gctcaacgca aacgtcaacg ttgatgtcgt atctaagtta gcaagcctcc
      781 tttcaacatc ggttacaaaa ttagtttcga tgcaataaaa atgaaaaaac acattaaaaa
      841 ccttttttta ttggctgcta tttgtttgac agtggcatgt aaagagcaat tgtatacagg
      901 attaacagaa aaagaagcta atcagatgca agcactgttg ctatccaatg atgtcaatgt
      961 ttccaaagaa atggacaaaa gtggaaatat gacactcagt gtggaaaaag aggattttgt
     1021 tcgcgcaata acgattttaa ataataacgg cttcccgaaa aagaaatttg cagatatcga
     1081 ggttattttc ccacctagcc agttagtggc atcgccatca caagaaaatg caaaaatcaa
     1141 ctatctcaaa gaacaagaca tcgaaaggtt gctaagtaaa atacctggtg ttatcgattg
     1201 cagcgtttca ttaaatgtta acaataacga aagtcaaccc tcctcagcag ccgttttagt
     1261 catcagctcg ccagaggtta acttagcacc atcggtcatt cagataaaaa acctagttaa
     1321 gaatagtgtt gacgatctca aattagaaaa tattagtgtt gttatcaagt catcctcagg
     1381 acaggacggg taatatgtat gaacaataat aatggcatag caaagaatga ttgtgactgg
     1441 ctaacggccc ttgattttgt aaaggatgtc aacggttcac caacgcatct cactttttat
     1501 atctaccaaa aaaatgcttt cttgcacgat tttggaaact actgggttct atatattgag
     1561 cttagtggtg attttcgtca ggttccaaca gatacattta tcagactctg caatatcctg
     1621 gccgttagta acgaatataa acaaatgggt attttcctgt ctaataagaa gtggtatctt
     1681 tgccaaattt ttcataaaga taataatcat cgcgcaaaca tgtcaaaagc aataatgcaa
     1741 catactctcg cctctttgct cgataagcaa tttgataaac tagagcaatt atcttcatcg
     1801 gatacaatga tgccgccaac acacttgt
//
LOCUS       DAZGXS010000320         1826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGXS010000320 DAZGXS010000000
VERSION     DAZGXS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..431
                     /locus_tag="ACHKG5_005012"
     CDS             <1..431
                     /locus_tag="ACHKG5_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870913.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFDKVKYPRLATAYPSGKLP
                     DLRGEFIRGWDDGRGIDTGRALLSIQSDEVRKLALKYWGPASNSSPSKTFALSDSAGG
                     GLYTDGISQASGGIINAFQLPGGNETRPRNVAFNYIVRAA"
     gene            431..1033
                     /locus_tag="ACHKG5_005013"
     CDS             431..1033
                     /locus_tag="ACHKG5_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0870914.1"
                     /translation="MDNAVLNSEFIATKAGNITVYNYDSETREYISTSTEYLAVGVGI
                     PACSCLDAPGIHKVGYAICRSVNLNSWEYVPDHRGEIVYSTETGESKEITAPGDYPEN
                     TTTIAPLSPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYTDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1005..1448)
                     /locus_tag="ACHKG5_005014"
     CDS             complement(1005..1448)
                     /locus_tag="ACHKG5_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0870915.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVSQNIISEFM
                     AEPPQGKIRIAGGDGLPAWADIPAPTHEELIEITESERQLLINQANDYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIKWPTPPAVQAR"
     gene            complement(1469..>1826)
                     /locus_tag="ACHKG5_005015"
     CDS             complement(1469..>1826)
                     /locus_tag="ACHKG5_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870916.1"
                     /translation="AKRNVGTGENQIPDMASFASGDGWMKLPNGKILQYGRGEAMPKL
                     STQTMRITFPIPFPKKADIAILTHSGDGGAPFGAGRGFVMSVEGPTLTGFNSAYRTAS
                     TSDKVSMTYSWWAVGE"
BASE COUNT          486 a          447 c          417 g          476 t
ORIGIN      
        1 cattacctgt tggtgtccct gttccgtggc cttcagctac gccgccaaca ggctggctga
       61 aatgcaatgg cgctgccttt gataaggtta aatatccccg tcttgctaca gcatatccat
      121 cagggaaact acctgatctc cgcggtgagt ttattcgtgg ctgggatgat gggcgcggca
      181 ttgatacagg gcgtgcttta ttgagtattc agagtgatga agtcagaaag ctcgcattaa
      241 aatactgggg accagcttca aacagttcac catcaaaaac atttgcactc agcgacagtg
      301 ccggtggcgg gttgtacacg gatggaataa gtcaggcaag cggtgggatt atcaacgctt
      361 ttcagctccc tggcggtaac gaaacccgcc cacgaaatgt tgcatttaac tatatcgtga
      421 gggctgcata atggataatg ctgtattaaa tagcgagttt attgccacga aggcggggaa
      481 tattaccgtc tataactatg atagtgaaac acgggaatat atttccacat caactgaata
      541 tcttgctgtg ggggtcggta ttccagcatg ttcctgtctt gatgcacctg gcatacataa
      601 ggttggttat gcaatctgtc gttctgtgaa tttaaattca tgggaatatg tgccagacca
      661 tcgcggtgaa atcgtctata gcacagaaac aggagaatca aaagaaatca cagctccggg
      721 tgattaccct gaaaatacaa ccactatcgc ccctttatct ccatacgata aatgggatgg
      781 tgaaaaatgg gtgaccgata ctgaggcaca gcatagcgcc gcagtcgaag cggcagaagc
      841 acagcgccag tcactgattg atgctgctat ggcttccatc agtctgattc aactgaaatt
      901 gcaggccgga cggaaactga cgcaggcaga aacaacccga cttaacgccg tgctggatta
      961 cactgacgcg gtgacggcaa cagataccag caccgcgccg gatgtcatct ggcctgaact
     1021 gccggaggcg taggccattt aatatctggc gcaccggaag tatcgaccag ttccagtgcg
     1081 tccagataat ccagccacag attatattgc gccagttcct cgcctttcag acgaccaata
     1141 gccgctttac caggccattg tttactgttc atataatcgt tggcctgatt aattagtagc
     1201 tgtctttctg attcagtaat ttcaataagt tcttcatgcg taggggcagg aatatccgcc
     1261 cacgcgggca gcccatcacc tccggcaata cggatttttc cttgtggcgg ttcagccata
     1321 aattcactga taatattttg actcacctcc ttagcatctg ataaatccca tccctctgat
     1381 ttatatttat caatcatatc cacagggaaa aaagcattat gccttgcgct ataaacatat
     1441 tcgttcatat aaatcatcct gaataaaatt attcaccaac agcccaccaa ctgtaagtca
     1501 ttgacacctt gtcgctggtt gatgcagttc tgtaagcaga attaaagccg gttaatgttg
     1561 ggccttctac ggacataacg aatccacgcc ctgcaccaaa aggcgcaccg ccatcgccag
     1621 aatgggtaag aatggcgata tccgcttttt tagggaaagg gataggaaat gtaatcctca
     1681 ttgtttgcgt cgataatttc ggcatagcct cacctcgacc atattgcagg attttcccgt
     1741 tgggtaattt catccatcca tcaccactgg caaacgaggc catgtccggt atctgatttt
     1801 cccctgtccc cacattccgt tttgcc
//
LOCUS       DAZGXS010000321         1820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGXS010000321 DAZGXS010000000
VERSION     DAZGXS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(84..959)
                     /locus_tag="ACHKG5_005016"
     CDS             complement(84..959)
                     /locus_tag="ACHKG5_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP0870917.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1100..1369)
                     /locus_tag="ACHKG5_005017"
     CDS             complement(1100..1369)
                     /locus_tag="ACHKG5_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0870918.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSFTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(1371..>1820)
                     /locus_tag="ACHKG5_005018"
     CDS             complement(1371..>1820)
                     /locus_tag="ACHKG5_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0870919.1"
                     /translation="AGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAG
                     PQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGALIT
                     GLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
BASE COUNT          425 a          477 c          367 g          551 t
ORIGIN      
        1 ctcccgccga caatattaat ggccaggaaa tgtttaaaac aatgattctg tttcctaacc
       61 atcaagtgta tatgctggct caattactca atatctatgg gttgagttct acccaaagtg
      121 gctctgtagt cgcttcttct tacagtatgt tcaggtgttg caggcacagt tgtggagcgt
      181 agatgcgttg ttggtttacc atgctctggt acataaaaaa cgccagtgcc atggtaacct
      241 gatatagaaa cgttaattcc gcgacgttca aactcggcat agacgtgttc tgctaaagac
      301 ctcttttgac caaataatgc cctggccaac cagccgttat tcatatttgc ggatttttct
      361 atttcatcag gagagaagtc tttgtttttt attatgttgg ctgagttaca ggatgttaat
      421 ctgatatcat ctatttcatg aagattatgc tgaacaatgc ggtcgacaat atctgacggt
      481 gataaaaatt catctccgca cttaagaaga ggtagaccag cagaaccatg cccagacaga
      541 taaattttgt tgtattttcc tagtgttaaa tcatctggca ggatggtttt catctctgtc
      601 gctgtaatac ttatagctgc agcagcaaca acatcgctat tacttgattg tagatggctt
      661 ttatttgctc caggatatgt aaactccatt ttccttttat caaaatcttg ttttgtagca
      721 tcattcagga ataataaatc atacggtttt ttccccgttg tgactcttga aacattttgg
      781 ccaagaatat taagagtata gtcattaacg gatttcattc ctaaaaaaac aagtaatcct
      841 tctttctctt gcgctttttc gattgttctc cctgctaaag ttattgggat ggattgagat
      901 tgttttgtcg gaactgatac gctggcgcta ataggtagta gggttcttat gctaaacata
      961 caacctcttt cttaattttc gaacctgttt aggattctgt gtaaattcaa aataaacctg
     1021 tctgaacatt cctaacaaac atccaccgga catgacaaca aaaaccggag ccggactccg
     1081 gtttttgtga agctgtcggc tatttcattc cgccaatatt ttcccacctc ccgtcagcac
     1141 gcagaatttg cagcggtctt accacgcact gtatctgctt tttatccgca tccagtatca
     1201 ccacctgcgt gattaccctg tcctgctccg gaataatacc attctcatcg gactccagga
     1261 tgtctgccgg ccccagtcgc agttgtgctg taaacgactg cacgtgttca cggccatcat
     1321 gctttccgca accacacaga cgctgcataa gtttttttag tatattcatg tcattctcct
     1381 gttctgcctg tatcactgcc cacttcattc agccccttaa catcctgcca cggcccgtca
     1441 ccaaacctga cctgcaaatg ccgaaacagc ccctgaacct gtgtggcatc tttggggtca
     1501 agaaaggtca gtccggtgat gagcgcacca tctgtatccg ggaaccagcc attgctgttt
     1561 gtctcaataa tgtttcccgg ccccagacgg aaccgtattt gcgtctcccc cgggtcgccc
     1621 ttcggcccct gaggtccggt tgcccccacc gggccagccg cacctgtttc tcctttcggt
     1681 ccctgtgggc ctgccgggcc tgccgcaccg gtatctccct ttggaccctg aggacctgca
     1741 tttcccgtca gaccggtctc tccccgctct cccctgtcac ctttcggccc ctgcgggcct
     1801 gccggaccag catcacctgc
//
LOCUS       DAZGXS010000322         1814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGXS010000322 DAZGXS010000000
VERSION     DAZGXS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1419
                     /gene="espN"
                     /locus_tag="ACHKG5_005019"
     CDS             <1..1419
                     /gene="espN"
                     /locus_tag="ACHKG5_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFP0870920.1"
                     /translation="EKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLIEL
                     SIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKE
                     SSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHPSNNAN
                     FYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYI
                     ESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKS
                     EGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHS
                     IKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMA
                     QQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVE
                     PLAIIDRTFGMKENISMVNASK"
BASE COUNT          591 a          278 c          389 g          556 t
ORIGIN      
        1 gaaaagaata ataaacaaaa agaggcaata gaaaagttca aaaaagaagt atcccatatg
       61 aatggcgggc agcagtttat ttgggggttt gataaggtta ttcaagaagg cttaagtgga
      121 ttgattgagt taagtatcga tattaatgat agtacaaatc atcgtgataa gtcttctctt
      181 tctcctgatg ggagagctgt gttacatttt ttaggtacaa tttggaatat ggcgatggga
      241 gctgtacctg gttataatgc attgtctggt gtaagtagca ttttacatag tgctatagta
      301 aaagaatcta gcaatatctg tgattatatt cagggggctg tacgtattgg aatggacttt
      361 gttccaggca ctcgctctga cttacatagc cgttcgctgc agataaaata tgaggctttg
      421 aagcatatag aaaaaaacat taatgataat attatttatc atccgagtaa taatgctaat
      481 ttctattctg taattgagtc aattgatggt aatgacttta tatataacga aaaacaatct
      541 aaaatattag aaatgaaaca ggatcgtggg gggaatagat atagtgcagt agatcttaac
      601 tcttctaagt atgggtatta tgagaaagtt ggcggtggtt tttatagata tatagaatcc
      661 tttaacccca tatcttcaga gacaccaaat aaaatagtct acaaggggga atcagtagat
      721 ttaactaagg agccaaattc ggaattatat tcaggtaggt attctataaa taacaaacag
      781 gttaatgttt atttctttcg tgacgctgat ggtacatttt ataaatcaga aggtcttcat
      841 ggtgggggag ttattagata catagataaa ccgtattctc agttaagaga aggagatatt
      901 gggtatgatg aggatttgtt ggatatatac gatgattctc cggtgcttga agacacgttg
      961 cctgctttat cttctgaaat agtaccaact ccagaacata gtattaaaca aatttattcg
     1021 aaaattaagg aggggcacat agaactgtcc gattcagaca tcatattgtg tcgcggcaca
     1081 accggtattc aagctgaaaa tatcgttgaa tataaaactg ctggagggct tcctgattca
     1141 aatccaaatg taaaagcacc agatgaatat atggcacaac agcaggtacg tattggaaga
     1201 atattgcctg aatacacatc ggatcttagc gttgctgatc ggtttagtcg tgagcattat
     1261 ctaatagttg ttaaagtaaa ggcaaaatat atcacacgag gaagtgttac agagagtggt
     1321 tgggttatag ataagaccgc acctgttgaa ccacttgcga taattgatag aacttttggt
     1381 atgaaggaaa atatctcaat ggtaaatgca tcgaaatagt ttttttacaa tctatgtcct
     1441 gcctcctctg gtaaaaacga tgctacatct tggagatgtt gcacggcagt acggtgttga
     1501 ccgatatata gtgaagtaca catcggtcaa cgaattacca ttgtcagcaa tatcatcctg
     1561 ataaactcag tactcgtgag ccgcttgatg acgggcggaa tagcccaaaa ggtaaacgat
     1621 ccgcatggca gtccgtcgga aagctgctgc ttcatacgac cgcttaaacc gtcagttagt
     1681 gtcagtatcg ctgaagatca gcttcttttg ctgatttact ctgtttttac ctgccctgat
     1741 gaaatactct ttaatcataa cattgattat atcgaaaata tattttttgc tatcattaag
     1801 aatatttata tgtg
//
LOCUS       DAZGXS010000323         1811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGXS010000323 DAZGXS010000000
VERSION     DAZGXS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..352
                     /locus_tag="ACHKG5_005020"
     CDS             <1..352
                     /locus_tag="ACHKG5_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0870921.1"
                     /translation="YPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQRLKLSLSG
                     RLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQPVSGGRLG
                     VKIYPIMLEGQVKK"
     gene            349..1170
                     /locus_tag="ACHKG5_005021"
     CDS             349..1170
                     /locus_tag="ACHKG5_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0870922.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1408..1770
                     /locus_tag="ACHKG5_005022"
                     /pseudo
     CDS             1408..1770
                     /locus_tag="ACHKG5_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373978.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          465 a          368 c          537 g          441 t
ORIGIN      
        1 ttacccgccg acggtgaaca cctactggcg acgtcgtggc agcacatatt ttgtatcaaa
       61 agccggtgag cgttatcgcc gggctgtggc gcttattgtt cgccagcagc ggctgaaatt
      121 aagcctgtcc ggaaggctgg cgataaagat tattgccgag ccaccggata agcgccgccg
      181 tgacctggac aatattctga aagcgccgct ggatgcgctg acgcatgcgg ggttgctaat
      241 ggacgatgag cagtttgatg aaatcaatat cgttcgtgct cagccagtat ctggtggacg
      301 tctgggggtg aagatttacc ccataatgct tgaagggcag gtcaaaaaat gaaactggaa
      361 gatttaccga aatactactc cccaaaatcc cccggcctga ctgatgcatc ggcctcaacg
      421 tcgaaagata cgctgagtat cactgatgtg atggccgcgc agggcatgac acagaattgg
      481 gctgagatgg ggttttctgc gttccttggg aaaatgggca ttagtatgaa tgacagagag
      541 cgggcaacag aattgctgac agaatatgca ctcagtcggt gtgatcgcgt ggcggcgtta
      601 agaaaactcc cggcagaaat aaaaccggca gtgatgcgta ttatggcttc gtatgcgttt
      661 gaagattatg cccgtagcgc ggcgagcaaa aaacagtgcc cctgttgtca cggaaaaaaa
      721 tttattgaaa gcgaggtttt tacaaacaag atccagtatc cggatggtaa gccgccagtg
      781 tgggcaaagt gcacaaaagg cgtgtatccg tcttactggg aggaatggaa aaaagtcagg
      841 gaggtggtaa aagttgcctg tccggagtgt ggagggaagg gggaggtttc caccgcctgt
      901 aaagattgtc gtgggcgcgg tgttgccatt catcgtgaag agtcggtaaa acgtggtatg
      961 cctgttatca gagactgcca gcgttgtggt ggtcgtggct atgaaagatt accttcaacg
     1021 gaggcattta atgccatatg taatgtaacc gatgccatat ctcttgatac atggaaaaaa
     1081 acagttaaac gtttttacga tacgctggtg gtgcagtttg atattgaaga agcatgggca
     1141 gaacaacaac tgaaaaaggt gaccagatag ctttgttgat ttttcccgaa tctgtggtaa
     1201 atttgcccta acgatgggcg ttttatgcct gacgttagaa gattttttac acccgtcgcc
     1261 aggcgggttt ttttatgact gaaatcacgc cagtacagta aacgcgctgg tggttgtgaa
     1321 taccggtctt tctgcttgct ggctttttcg acaagagtta ttggtgtgtc acgttaaccg
     1381 gaaaaaggaa agtttgagaa acgcgatctg gcacaggcgg tgattaacgc cgcatacctg
     1441 gtggcctgtg cagatggtga atgtgaggct tcctagaaag cgaagatcga acaggtactg
     1501 cgtaatcagc cagcgctgtc cgcgtttacg tcagaaatta atgcgattag cgcaaccatt
     1561 atcggtcagc tggatacgaa ctttaaaact ggtcgtcgtg cggcgttacg tgagatcgag
     1621 gatgtgaaac acgatacgcg tgaagcggaa gatgtgctgg atgtggcggt ggccattgcg
     1681 gaggcagacg gcgaaattga gccggaaggg cgcaaggtgc tggaagagat tgccggtgtt
     1741 ctgggtcttc gtctggagaa ttacctgtga cggtaaaact gcgtctggct gtggctgcac
     1801 tcctgctgtt t
//
LOCUS       DAZGXS010000324         1796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGXS010000324 DAZGXS010000000
VERSION     DAZGXS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            266..850
                     /locus_tag="ACHKG5_005023"
     CDS             266..850
                     /locus_tag="ACHKG5_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0870923.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(875..1312)
                     /locus_tag="ACHKG5_005024"
     CDS             complement(875..1312)
                     /locus_tag="ACHKG5_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0870924.1"
                     /translation="MLKIRMSRPSEAPEIIQIWKNSVDATHDFLTAHDRQEIEKEVVS
                     FFSETPVLVATNQEDQPLGFMFLHEGHLEALFVDASARGLGVGKHLISHALAQHPDLS
                     VDVNEQNHQAVGFYQHMGFKLSGRSERDNQGRPYPLLHLYNAM"
     gene            complement(1586..>1796)
                     /locus_tag="ACHKG5_005025"
     CDS             complement(1586..>1796)
                     /locus_tag="ACHKG5_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870925.1"
                     /translation="PYGVTVYKNHEFNSLIFNDKNHFIVFLLAKINFIQPFDFIDVRF
                     YFGERFSLPFISKLQVYILLTSQYM"
BASE COUNT          557 a          339 c          366 g          534 t
ORIGIN      
        1 cgcatattag caaaaaaaat attattttag gtatttctac agtacatttg acacgatgat
       61 taacctctat gtagctaggt ttttgtaaag aaactgtgcg tcactgtatc caggactgtt
      121 atgtgccaaa taaggaagtt gaagttacca gctcttactc taaagctata acgtatagaa
      181 aaaatgcatt tgagacgtgt aaaatcattt atttaatcgc aatttatgta atggcataat
      241 caggtcggtg aaagagggga gaagaatgga taaaagagct aaaaatcaga tagtcgatag
      301 cgatattgca cgtttgttac tcaagctcag aaagtctcga aacttgactg ttacagagct
      361 tgcccaacga tctggtgtca gtcaggcgat gatcagcaaa gttgaacgag gcacttcaag
      421 ccccagtgca acaatactta gtcggttagc taatgcactg aatattacgc tatcaaagct
      481 ttttgcagag cttgagatgc aacagaattc acttgtactt ttggctgacc agcaacaaca
      541 ttggattgat gaagaaacag gcattacccg ctggtctttg tccccagcgg gtgcatgtcc
      601 ggaactaata aaagttgaaa tcccccctat ggggcaattg actatccctg cctctgcaaa
      661 tgagcatctt tctggacaaa ctctatggat gttaagcggc agccttgatt ttcgagtcaa
      721 tcatcagact catcatttac aagccggtga ttgtcttgct cttacctttg ccagcgagta
      781 ccaaatgaga aacccagata gcggccagag ttgttcttac attgtcgctt tcagccaaaa
      841 aaatccatga tgtaattctc ctaaaattat catgtcacat tgcgttataa agatgtaata
      901 gagggtaggg tctgccttga ttgtcccgct cggaacgtcc tgaaagttta aagcccatat
      961 gttgataaaa gcctacagct tgatgatttt gttcatttac atcaacgctt agatcaggat
     1021 gttgagccag cgcatgtgag atcagatgtt tcccgacgcc aagtccgcga gctgacgcat
     1081 caacaaaaag agcctctaaa tgtccctcat gcaaaaacat gaatcctagt ggctgatctt
     1141 cctggtttgt tgctaccagc acaggggttt ctgaaaaaaa gctgacaact tctttttcaa
     1201 tctcctgccg gtcatgggct gtaagaaaat cgtgtgtggc atcaacggaa tttttccaaa
     1261 tttggataat ttctggtgct tcagatggtc gcgacattct gatttttaac atgttatctc
     1321 ccttaaagtg gtcgttgctt gtgtcttgag atacaaaacg agttatcaaa tttactatag
     1381 tgaaattaaa ttcacaatag tgatttggtt ccagtgcctt aacattaccc atgaggagta
     1441 caattaaacg ggttaatcag tatctccatt tgaaagaaaa aagacttctg tactgtttga
     1501 tatcaccgct attttcttat ttgctgtgca ggtcgtagtg ggacaaaact gagacacata
     1561 aggtctagca atggcttgca aggcctcaca tgtattgaga tgttaataaa atgtagactt
     1621 gtaattttga tataaatggt agagaaaatc tttccccaaa ataaaaacga acgtcaatga
     1681 aatcaaacgg ttgaataaag ttgattttgg ctaataaaaa gacaataaaa tgatttttat
     1741 cattaaatat caatgagttg aactcatggt ttttatatac tgttacgcca tatggg
//
LOCUS       DAZGXS010000325         1790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGXS010000325 DAZGXS010000000
VERSION     DAZGXS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..160)
                     /locus_tag="ACHKG5_005026"
     CDS             complement(<1..160)
                     /locus_tag="ACHKG5_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870926.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAV"
     gene            complement(162..440)
                     /locus_tag="ACHKG5_005027"
     CDS             complement(162..440)
                     /locus_tag="ACHKG5_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870927.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(608..763)
                     /gene="hokD"
                     /locus_tag="ACHKG5_005028"
     CDS             complement(608..763)
                     /gene="hokD"
                     /locus_tag="ACHKG5_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0870928.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1007..1111)
                     /locus_tag="ACHKG5_005029"
     CDS             complement(1007..1111)
                     /locus_tag="ACHKG5_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870929.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            complement(1221..1784)
                     /locus_tag="ACHKG5_005030"
     CDS             complement(1221..1784)
                     /locus_tag="ACHKG5_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870930.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
BASE COUNT          418 a          486 c          434 g          452 t
ORIGIN      
        1 gaacggcagg aacgaccccg gacggcagat tacgcatgct tttcggttcc ggctccacca
       61 gtacccgggt attgtggaat accggcatgg attcacggcc cggcttaacg atcaccagcc
      121 cgagttccgg taccagaaca ggtcgaagta atacccgcac gttacctcca gatgcgttgc
      181 tggaatgtgc gggacggacg cggtgggcgt tcggagtaag gaagcctgac ggagattatc
      241 cagtgacgat aatcgagact gagggctttc ttaacctcgt atccgcgcct gcggtaacac
      301 tgaatcagcc attcagcctg ttcttcggtg caggggtcgt gctgatacca gtcagatttg
      361 aatgcatgag aacgccgccc gtgcctgctg gcaggggcgg cagagttatc cgaattgtaa
      421 aatttggtat cgtgcgccat ctgttttctc tgctggcgca gcaggtgcca gttgttcagg
      481 ctgacggatg gattgtaaac cagaacgacc agaaaaaaca aaacccgccg aagcgggtta
      541 agtgcgggtg cgttgaggat gcctgacaca tcagcggtgg cgagggattt ctcccccgcc
      601 tggtctctta ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc tggccggttc
      661 ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg acggttaaac
      721 agatgacgat cagggcgatt aacatcgcct tttgctgctt cataacctgc ttctccttgc
      781 ctttcggcgc gtaagaggct aacctacgtt tgtgaagcat agattgggcc tcagattaat
      841 gttaagcgtc ttgcaggacg cgtaatgtta actggggctt ttctctgtct gccttacggt
      901 ggcatgcccg aggcagacag cctcaagcac ccgcagcaat tctacttaac tctcgcttta
      961 cagcaaaccg ttttgcccga tatgggaatt cccatacgga atgaattcag ttccccaggc
     1021 gctccatcaa aaacacaacc aggcagtaaa cacccacaac agcaataaca gccagagcgc
     1081 cttccattac cagtgaaata tcatccgaca tattccctcc cttggtgtga atcccggcga
     1141 acgtttttac ccccaccgac aaataacata tactagaaaa gcaatagcta tagcaacgcc
     1201 tgcaaatgca tctggccggc tcattggttc tccccctgtg tcgcttctac tgcgatctga
     1261 ctggcgtatt cgttaatggt aacgataagt tcttgctcgg cctcatccag acaactaccg
     1321 atacctcgcc tgtccacttc agaagcatcg aaatctgcac gaagcctggc gaccttcagg
     1381 attgcggaca acacctcatc agggattgcc ggagagttgg ttgacgtttc cgagattatc
     1441 cgaaaattat tggttgacga acccttattt tcccgaaagt ttccagcctg aagcatggcg
     1501 gcgcggtgac accagataat ccagccaagc gccatatccc atgccatgta ttctctatcg
     1561 ccattttttg ctctgcggcg atctacagat tccccgaaac gcttctccat aaataattca
     1621 taggctgccc gttcatccga tactgatgcc agtgatgcca gtgcaatttt taatgcggta
     1681 agcatgttgt tttgatcttc atcgagtccg aacggtattt catcccgtga tgactcaatt
     1741 ccggtaatcg tgttctgtag ccattctttg gttaattcag tcatttttca
//
LOCUS       DAZGXS010000326         1786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGXS010000326 DAZGXS010000000
VERSION     DAZGXS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            118..270
                     /locus_tag="ACHKG5_005031"
     CDS             118..270
                     /locus_tag="ACHKG5_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0870931.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            751..939
                     /gene="dicB"
                     /locus_tag="ACHKG5_005032"
     CDS             751..939
                     /gene="dicB"
                     /locus_tag="ACHKG5_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0870932.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            936..1124
                     /locus_tag="ACHKG5_005033"
     CDS             936..1124
                     /locus_tag="ACHKG5_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0870933.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            1220..>1786
                     /locus_tag="ACHKG5_005034"
     CDS             1220..>1786
                     /locus_tag="ACHKG5_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP0870934.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQ"
BASE COUNT          504 a          401 c          435 g          446 t
ORIGIN      
        1 ggtcaggggt taagtagcca gcccgaggcg tatgaacatg acggcgggaa cactttgtat
       61 aacagcgcag caggttttta gttccgcgac ccggcgttaa gggtaaatga ggtcaacatg
      121 gatatgctca atcttggcaa caatgaatct ctggtgtgcg gagtattccc caaccacgac
      181 ggcacgttta ccgcgatgac gtataccaga agtaaaacgt ttaaaaccga agctggcgcg
      241 catcgctggt tagcaagaaa cgctaactga ttagcgccag taaaaacagg tttccacagg
      301 ttaatttacc ctgaaaagtc agggcataac acgaaagcgc acggcgaagt tagtctctct
      361 gtataggtcg tcgttaaatt taattcgacc gtgcgcttct ggttgtggca atccgcgaaa
      421 tggcgcggcg gtaagtatgg cggggttatt ccttcccccg ttgaggacac cgggttgtca
      481 ggttgaccat acgcttaagt gacaccccag ccacaacaac ctctgttatc acttttctgg
      541 tgattcggcg gaaatggata tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      601 ggctatgcgc acgcggaaca gttaaagcag taaggcggtc ttttactggc gtaacgagca
      661 tcaactaacc cgacgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      721 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
      781 tctgaaggtt gttttgatat tggtgttctg ctcagtaata aagcgtttac tgaggatgcc
      841 attaatatgc ggaaatatga gccttatctg ctcaatgata attccattct ttcccgaatt
      901 gcccttatta aacttggtat tttcggagaa cgtcaatgac ttcagcattt gcactggtga
      961 tgacggtttt tcttataacg ggtgaatcac agaatgttat taccggaatt tatgccagta
     1021 aagaatcctg cctccaggca agagacgagc aaaaaatttc tggtgaatgc ctaccggtaa
     1081 aaaaagtatc gctgtacctg aataacgaaa caccggctgg ataacccgcc agccatatta
     1141 acgccatacc aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct
     1201 gtaatgaggt taaaacacaa tgagtaaaat ctttatttgc gctgctattc ctgacgaaca
     1261 ggccataaaa gaagatagcg ctgttgcggt ggccactgcc attgaagccg gtgatgagcg
     1321 tcgcgcacgc gcaaaatttc actggcaatt tctggagcaa ttcccggcag ctcaggactg
     1381 cgcttataaa tttattgtct gcgaggataa acccggcata ccccgccctg ccctcgattc
     1441 ctgggatacc gaatatatgc aggaaaaccg ctgggatgag gagtcagctt cctttgtacc
     1501 ggtcgaacca gaatccgatc cgatgaacgt caattttgac aagctgtccc ctgaagtaca
     1561 gaacgcagtc ctggttaaat tcgacacatg cgaaaacatc accgttgata tggtgattag
     1621 cgcacaggaa ttactacagg aagacatggc aacattcggc ggacatatcg ttgaagcgtt
     1681 gatgaaaatg ccagaagtta acgccatgta tcctgaactt aaactgcatg ccatcgggtg
     1741 ggttaagcat aaatgtgagc ctggcgctaa atggcctgaa attcag
//
LOCUS       DAZGXS010000327         1736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGXS010000327 DAZGXS010000000
VERSION     DAZGXS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            101..1201
                     /gene="ompC"
                     /locus_tag="ACHKG5_005035"
     CDS             101..1201
                     /gene="ompC"
                     /locus_tag="ACHKG5_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0870935.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1364..>1736
                     /locus_tag="ACHKG5_005036"
     CDS             1364..>1736
                     /locus_tag="ACHKG5_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0870936.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKS
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     DNASLVNINLSFNKINTITFPN"
BASE COUNT          525 a          352 c          351 g          508 t
ORIGIN      
        1 atcaccattc taatttaaaa acagtatgtc atttataaat gacgccgttt aaaattcgtt
       61 taaaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa aaatagttgc
      121 ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag tctataacaa
      181 ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt ttactgatga
      241 taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat
      301 caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct ataacgatga
      361 agccaacggc tcgcgcgaca acaagacccg tctggcctat gctggtttaa aaattagtga
      421 atttggctct ctggactatg gccgtaacta cggtgtcggc tatgacattg gttcatggac
      481 tgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct tcatgacata
      541 ccgtaccacc ggtgtagcaa cctatcgcaa ctacgatttc tttggcttaa ttgaagggct
      601 gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc atctttatgg
      661 tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt atgtttatga
      721 tggctttggt atcggagcgg tgtataccaa atccgatcgg acaaatgcgc aggaaagagc
      781 cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctgtgggcta caggtataaa
      841 atatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa acatgaccac
      901 ctatggcgat ggttatattt ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca
      961 attcgacttc ggcttgcgcc cctcactcgc ttacctgaaa tcgaaaggca tagatctggg
     1021 ccgctacggc gatcaggaca tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa
     1081 caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa gcgactttac
     1141 ccgtgccgta gatattcgca ccgataacat cgtcgctacg ggcattacct atcagttcta
     1201 acatcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc tgctgtatga
     1261 ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca attaatatta
     1321 ttccataccc ccggggacat cttactataa tatttattga tgagtgatta tgaccaatat
     1381 taatacagct tgtgtaaaaa ataatgccag ttatcaattt aataacgcat tacctaacaa
     1441 agaaaccatc tccagcaatt tttgtgaacg actagaacaa tggggtaata agtcgcttaa
     1501 taatggtgaa gaaagagcaa ttgccgtgga gcgaattaaa gaagcttaca attcgaacat
     1561 ggcatctctt gatttgtcat atcttgattt gagtgagtta ccgcctatcc cctcgacagt
     1621 taatacgtta aatctggaaa acaattgtct aacttgtctt gactttactg acaatgccag
     1681 tctcgttaat atcaacctca gctttaataa aattaacacg ataacctttc caaatg
//
LOCUS       DAZGXS010000328         1735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGXS010000328 DAZGXS010000000
VERSION     DAZGXS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            154..537
                     /gene="cesF"
                     /locus_tag="ACHKG5_005037"
     CDS             154..537
                     /gene="cesF"
                     /locus_tag="ACHKG5_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFP0870937.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQINSILEMNSNRELLGMFFLSLKDDNILLNIAFPADKIDITEFAN
                     LMENGYLLKNEIIRSLSSRPTDFLP"
     gene            complement(735..1241)
                     /gene="espH"
                     /locus_tag="ACHKG5_005038"
     CDS             complement(735..1241)
                     /gene="espH"
                     /locus_tag="ACHKG5_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFP0870938.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(1272..>1735)
                     /gene="escQ"
                     /locus_tag="ACHKG5_005039"
     CDS             complement(1272..>1735)
                     /gene="escQ"
                     /locus_tag="ACHKG5_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP0870939.1"
                     /translation="NWQLVPHSNALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQ
                     ERRCWIVINTPLATVNLSDNNLSVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHM
                     LRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIVKINEDFYFTVSIVCD"
BASE COUNT          554 a          345 c          292 g          544 t
ORIGIN      
        1 tctacaggta gttgcactac atttatcata tgctatcatg aatatgcaac actattcccg
       61 taatcttata tctacgcctt attatttgta tcagaactct cggattaatt acgtttgagt
      121 atatcaacat gaaaagaatg gcaggactac aatatgaatg agaaatttcg cacagacctt
      181 gcacatacgt ttgggatagc tcttgaagag caaactgacg tgctctcttt ccacgataac
      241 gatggccatg aatggatact ggagtgtgca tctcaatctg aaattctgtt tttttattgt
      301 tatttattga atagcgaatc aatacagatt aattcaatac ttgaaatgaa tagcaatcga
      361 gaactgctcg gtatgttttt cctgtcatta aaagatgaca acattctttt aaatattgca
      421 tttcctgcag ataaaattga tattacagag tttgctaatt taatggaaaa tggttatcta
      481 ttaaagaatg agataattag atccttgtct tccagaccta cagatttttt accttgataa
      541 tttctgcgct ggatgggatc atccattccg tttaacatac tcttcatcac agggacctct
      601 gatttctggc cgctaacgtc tctgaagatt cagcctgtat tatctaaaag caatgtaata
      661 tcagttaaaa agcggaaaaa ctccagaatt ataactattc tggagttaag tcaggaagaa
      721 aactcaaatg ctaatcataa tacgctataa gaggaagctc ttgttgctat agccctatta
      781 tcaagacgtt cggtagactg tacacgacgt aacaaagaag ataatcctct ttcagctaag
      841 atcccctcca ggggagtacg aggaaaccag gttgaagaaa gtggagttat atgtctctca
      901 tttaattttc tttccaaagc cttagtcaca ccgccagaaa acacgctaaa tagcgttgag
      961 aaacatccat tttccggtgt tattctttgt actttgtaca cataatcatc ttttgggcca
     1021 tctttaataa agtgaacagc atagtctttg cctctaaaag agacaatggt ggaatcccct
     1081 atttctggat atacccgatg atggcgttgt tgcattcggg ctttaagctg aatttcatct
     1141 acgatatctt cattgggtat atgaagcctt ctgttgaacc tgttccatga cgcatgttca
     1201 cttgtcagaa atgtcttgaa taccgctcct gataacgaca tgatacatct ccctatataa
     1261 cgtattcctg attaatcaca tactatgcta acagtaaaat aaaaatcttc gttgattttt
     1321 acaatactcc cgtatgctat cgtcgtatta ttgcgtatta actgacaacc accaaaaaga
     1381 tggtatccat ctgaattaat aatgtcatct tttttaacat tacgcaacat gtgtatatca
     1441 atatggactg taccgatcgc acaataaatg cgattcatga cagtctcatt gctaagagca
     1501 gataacagat ccgcagccag aacatccgtg acagataaat tattatcact cagatttact
     1561 gttgctaaag gggtattaat aacaatccag caccttctct cttgactatc atcaacatga
     1621 tagagtctca gactatcacc aactctcaac tctgtaactg gataatcagt ccacccaggt
     1681 acgatatagc catcaaaaaa aagagcatta gaatgtggaa ctaattgcca attat
//
LOCUS       DAZGXS010000329         1732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGXS010000329 DAZGXS010000000
VERSION     DAZGXS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..354
                     /locus_tag="ACHKG5_005040"
     CDS             <1..354
                     /locus_tag="ACHKG5_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0870940.1"
                     /translation="AIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVA
                     QMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTR
                     DEGGDGKVVVIGRQA"
     gene            complement(545..1072)
                     /gene="sodC"
                     /locus_tag="ACHKG5_005041"
     CDS             complement(545..1072)
                     /gene="sodC"
                     /locus_tag="ACHKG5_005041"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0870941.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1206..>1732
                     /locus_tag="ACHKG5_005042"
     CDS             1206..>1732
                     /locus_tag="ACHKG5_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870942.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRP"
BASE COUNT          405 a          429 c          505 g          393 t
ORIGIN      
        1 gccatcgtgg ggtcgttctt cactgccggg gcatcaatgg cgttatgggg ttcagccctg
       61 gcagccggtg gtttttctgc caccacgatg ctgttttcac ttggagccag catgattctg
      121 ggcggtgtgg cccagatgct ggccccgaag gcaaaaacac cggattaccg cgcaacggat
      181 aacggcagac agaacacgta cttttcctcg ctggataaca tgattgccca ggggaacccg
      241 atgccggtgc cttacggtga aatgctggtt ggctcccggc gaatctccca ggacatcagt
      301 acccgtgatg aaggcggtga cgggaaggtg gtggttatcg ggcggcaggc ataaaagcga
      361 aaaaatcccg cagtgctcac ggacaggaac tgcgggagcg ttacgaagat tgagtgtaag
      421 gaattattct tatgtcacga caaaaacatt aactcagaga gggaggatgt gccgttcttt
      481 tcagggagaa aggatttatc gtcctgagga ataaaggtaa ggggcccgcc ccttacctga
      541 ctgattattg aatgatgccg caggccattc tcgcaccacc accgcccagg ggctccggat
      601 ggtcatgatg gttatcaccg ccagcatgaa gcatgagaga acgcccttta atctctttta
      661 atgagttcag tctcggggcc aggaccgggt agttcgcttt tccgtcatgc gtcacgaaca
      721 gcgcagggag gtcgcccagg tgtccatccg gagaccaggg gccaagatgt ttgccggtgt
      781 ttttcgggtc aaagtgaccg ccagccgata atgctgcgac cggttttccg tctttcagtg
      841 ccggggcgca atttcctttt tcgtgcacat gaaaaccatg aatgccttca gacagagagt
      901 gaagggctgg tgtgaacagc agaccgtagg gggtctcctg aatggttatt tttccaatgc
      961 tgacttcttt tccgtcagca ctgacaaggt tcattgggac ttcctgttct gctgcgtatc
     1021 cgcatgatgc tgctgtcagc atggcaatgg cagcaatgat tttacatttc ataaaaccct
     1081 cattaattcc gttaacagac tgagcttgct ggttacaggg taacaaacag cgttctgatg
     1141 atatcgcgca atagctgtgc aatatcctat cactgcgatt aataatacca attgagagga
     1201 acattatggg taaaggtggc ggcagggcgc acacgccggt tgaggcaaag gacaatctta
     1261 agtccacgca gatgatgagc gtgattgatg ccattggtga agggccgatt gaaggtccgg
     1321 tgaaggggct gcagagtatc ctggtgaaca aaaccccgct gacggacacg gacggtaatc
     1381 ctgtgataca tggtgtgaca gcggtctggc gcgccgggga gcaggagcag acaccaccgg
     1441 aaggctttga gtcttccgga gctgaaaccg cactgggcgt ggaagtgacg aaggcaaagc
     1501 cggtgacgcg caccattaca tccgcgaaca ttgaccgcct gcgggtcacc ttcggggtgc
     1561 agtcactgtt ggagaccacc tcaaagggcg accgtaatcc ctcttctgtc cgactgctga
     1621 ttcagttgca gcgtaacggt aactgggtga cggaaaagga tgtcaccatt aacggcaaga
     1681 ccacctcgca gtttctggcg tcggtgattc tggataatct gcctccccgt cc
//
LOCUS       DAZGXS010000330         1690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGXS010000330 DAZGXS010000000
VERSION     DAZGXS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1690
                     /locus_tag="ACHKG5_005043"
     CDS             <1..>1690
                     /locus_tag="ACHKG5_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0870943.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DAZGXS010000331         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGXS010000331 DAZGXS010000000
VERSION     DAZGXS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1676
                     /locus_tag="ACHKG5_005044"
     CDS             <1..>1676
                     /locus_tag="ACHKG5_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870944.1"
                     /translation="GENAGKTAILSNGAKYNPTTFSPVDAQTVEQLKMTAEIVCSVFR
                     VPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDEALETGENESTEFDVTT
                     LLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDALYLQQQNYSLEALSRRD
                     AREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRGDTEKMTERELSIIRAL
                     GEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVTVDEVRPVLQAMVDDAV
                     GAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIP
                     VPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRD
                     GRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDY
                     DPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEK
                     THGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYR"
BASE COUNT          388 a          399 c          548 g          341 t
ORIGIN      
        1 ggcgaaaatg cggggaaaac ggccattctg agcaacgggg caaaatacaa ccccacgacg
       61 ttttcaccgg tggatgcgca gacggtggaa caactgaaga tgaccgctga aattgtctgt
      121 tcggtgttcc gtgtcccggc ctacaagatt ggcgtgggac aaccgccttc cagtgacaac
      181 gtggaggcgc tggagcagca gtattattcc cagtgcctgc agacgctgat tgagtccatt
      241 gaactgttac tggatgaggc gctggaaacg ggggaaaacg agagtacaga atttgatgtc
      301 accacgctgc tgagaatgga cagtgagcgg cgcatgaaaa cgctggggga tgcggtgaaa
      361 aatacgcttc tcacgcccaa tgaggcccgt aaacgggaga acctgccgcc cctggccggc
      421 ggtgatgcac tgtatcttca gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat
      481 gcccgtgagg atccgttcgc gtctgccggg aaaacagttt catcacagct gcctgacggc
      541 gcatctgacg gtaataaggc aatcagtgaa acagagcatg atgcggtgaa agcgatgttc
      601 aggggggata ctgagaaaat gacggaacgg gaactgtcca ttattcgtgc actgggcgaa
      661 gaattttcca cagtgctggc ggatttacag cgcacatttg aggggaagat ggcctcgcag
      721 gcacaagcgt ttgaagagaa actgacttcc ctgtcggcgg tattacagaa gcatgtgacg
      781 gtggatgagg tgcgtccggt tctgcaggcg atggtggatg acgctgtggg ggccattccg
      841 gtaccgcgtg atggtcgtga ttatgatccg gatgtactgc agcaggcggt gaatgatgcg
      901 gtcgcaaata ttccgcagcc ggcggacggt aaaagtctca ccccggatga tgtgcgtccg
      961 atgcttgaac agatggtgaa ggaggctgta agccatatcc ctgttccgcg tgatggtcgt
     1021 gactacgatc cggaagtact gcagaaggcg gtgaatgatg cggtcgcaaa tattccgcag
     1081 ccggcggacg gtaaaagtct caccccggat gatgtgcgtc cgatgcttga acagatggtg
     1141 aaggaggcgg taagccatat ccctgttccg cgcgacggtc gtgactatga tccggaagta
     1201 ctgcagaagg cggtgaatga tgcggtcgca aatattccgc agccggcaga cggtaaaagt
     1261 ctcaccccgg atgatgtgcg tccgatgctt gaacagatgg tgaaggaggc ggtaagccat
     1321 attcctgttc cgcgtgatgg tcgtgactac gatccggatg ttctgcagaa ggcggttctg
     1381 gatgcggtga gtgccctgcc ggctccgcag gacgggcgtg atgccacggc tctggaaata
     1441 ctccccgcca ttgacgatca aaaatccttt ccccggggca cgtatgccac acaccagggc
     1501 ggactctggc gggcgtatga aaaaacgcac gggatgcggg gatgggaatg cctggttgac
     1561 ggggtggcgg atattgacgt cagcatgacg ggtgagcggt tgttctctgt ggtggtccgg
     1621 cagagcagtg gccagcgtac ggaaaaaaca ttttccctgc cggtgatgct ctaccg
//
LOCUS       DAZGXS010000332         1671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGXS010000332 DAZGXS010000000
VERSION     DAZGXS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..209
                     /locus_tag="ACHKG5_005045"
     CDS             <1..209
                     /locus_tag="ACHKG5_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0870945.1"
                     /translation="FGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAW
                     RHRHWHVSAFTGIYNDLAAASACM"
     gene            206..784
                     /locus_tag="ACHKG5_005046"
     CDS             206..784
                     /locus_tag="ACHKG5_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0870946.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            1025..>1671
                     /locus_tag="ACHKG5_005047"
     CDS             1025..>1671
                     /locus_tag="ACHKG5_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0870947.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTA"
BASE COUNT          403 a          419 c          523 g          326 t
ORIGIN      
        1 gctttggcgc atcggtggcc aatcatgccg ccatatactg cggcaacggt gaactgcttc
       61 accatctgcc tgaacaactg agtaaacggg agaggtattc cgaaaaatgg caacgacgaa
      121 cgcattcagc ctggcgtcac cgccactggc acgtatctgc cttcacgggg atttacaacg
      181 atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg gaagccatcc gtgccctgtc
      241 gatgcagatg ccgggattcc gccgtcagat gaacgaaggc tggtaccaga tacgtattgc
      301 cggtgatgac acggcaccgg aggcggtgta cgcccgtctt cacgaacagc tgggtgaggg
      361 aacggtcatc cacattgtgc cgcgactggc cggggccgga aagggtggac tgcagattgt
      421 gctgggggcg gcagccatcg tgggctcttt cttcacggcc ggggcatcaa tggcgttatg
      481 gggttcagcc ctggcagccg gtggtttttc tgccaccacg atgctgtttt cactgggggc
      541 cagcatgata ctgggcggtg tggcccagat gctggcaccg aaggcaaaaa caccggatta
      601 ccgcgcaacg gataacggta aacagaacac gtatttttcg tcactggata acatgattgc
      661 ccaggggaac ccgatgccgg tgccttatgg tgaaatgctg gttggttcac gacggatatc
      721 ccaggacatc agcacccgtg atgagggcgg agacgggaaa gtggtggtta tcgggcgggg
      781 atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga tgaagattaa
      841 cgttatggag ttatttttca ggcatcaaaa aagtaatgca gcgtcattat tgcggctaca
      901 ggcaattgcc ggaaatgtga agagtttcag aaattttatt ccgtcatgac acaggcaccc
      961 ttcggggtgc ctgttgtttt ctggcataaa cagattcaga catcagacag gagaggggga
     1021 cagagtgggt aaagggggcg gcaaggggca cacgccggta gaggcaaagg acaatcttaa
     1081 gtccacgcag atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt
     1141 gaaggggctg cagagtattc tggtgaacaa aaccccgctg acggacacgg acggtaatcc
     1201 cgtgatacac ggtgtgactg cggtctggcg tgccggggag caggagcaga caccaccgga
     1261 aggctttgag tcctccggag ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc
     1321 ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca
     1381 gtcactgttg gagaccacct caaagggcga ccgtaatcac tcttctgtcc gactgctgat
     1441 tcagttgcag cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac
     1501 cacctcgcag ttcctggcgt cggtgattct ggataatctg ccgccccggc cctttaacat
     1561 ccggatggtc agggagacgg cggacagcac cacggaccag ctgcagaaca gaacgctgtg
     1621 gtcgtcatac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg c
//
LOCUS       DAZGXS010000333         1670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGXS010000333 DAZGXS010000000
VERSION     DAZGXS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1660
                     /gene="tir"
                     /locus_tag="ACHKG5_005048"
     CDS             <1..1660
                     /gene="tir"
                     /locus_tag="ACHKG5_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP0870948.1"
                     /translation="GHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTP
                     VRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVF
                     RVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMV
                     TVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSS
                     LRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATRDQ
                     LTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQA
                     QKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTT
                     TTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQN
                     PYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAG
                     IQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          461 a          372 c          446 g          391 t
ORIGIN      
        1 tggtcataat cccaatgtga ataattcaat tcctcctgca cctccattac cttcacaaac
       61 cgacggtgca ggggggcgtg gtcagctcat taactctacg gggccgttgg gatctcgtgc
      121 gctatttacg cctgtaagga attctatggc tgattctggc gacaatcgtg ccagtgatgt
      181 tcctggactt cctgtaaatc cgatgcgcct ggcggcgtct gagataacac tgaatgatgg
      241 atttgaagtt cttcatgatc atggtccgct cgatactctt aacaggcaga ttggctcttc
      301 ggtatttcga gttgaaactc aggaagatgg taaacatatt gctgtcggtc agaggaatgg
      361 tgttgagacc tctgttgttt taagtgatca agagtacgct cgcttgcagt ccattgatcc
      421 tgaaggtaaa gacaaatttg tatttactgg aggccgtggt ggtgctgggc atgctatggt
      481 caccgttgct tcagatatca cggaagcccg ccaaaggata ctggagctgt tagagcccaa
      541 agggaccggg gagtccaaag gtgctgggga gtcaaaaggc gttggggagt tgagggagtc
      601 aaatagcggt gcggaaaaca ccacagaaac tcagacctca acctcaactt ccagccttcg
      661 ttcagatcct aaactttggt tggcgttggg gactgttgct acaggtctga tagggttggc
      721 ggcgacgggt attgtacagg cgcttgcatt gacgccggag ccggatagcc caaccacgac
      781 cgaccctgat gcagctgcaa gtgcaactga aactgcgaca agagatcagt taacgaaaga
      841 agcgttccag aacccagata atcaaaaagt taatatcgat gagctcggaa atgcgattcc
      901 gtcaggggta ttgaaagatg atgttgttgc gaatatagaa gagcaggcta aagcagcagg
      961 cgaagaggcc aaacagcaag ccattgaaaa taatgctcag gcgcaaaaaa aatatgatga
     1021 acaacaagct aaacgccagg aggagctgaa agtttcatcg ggggctggct acggtcttag
     1081 tggcgcattg attcttggtg ggggaattgg tgttgccgtc accgctgcgc ttcatcgaaa
     1141 aaatcagccg gtagaacaaa caacaacaac aactactaca actacaacta caagcgcacg
     1201 tacggtagag aataagcctg caaataatac acctgcacag ggcaatgtag atacccctgg
     1261 gtcagaagat accatggaga gcagacgtag ctcgatggct agcacctcgt cgactttctt
     1321 tgacacttcc agcataggga ccgtgcagaa tccgtatgct gatgttaaaa catcgctgca
     1381 tgattcgcag gtgccgactt ctaattctaa tacgtctgtt cagaatatgg ggaatacaga
     1441 ttctgttgta tatagcacca ttcaacatcc tccccgggat actactgata acggcgcacg
     1501 gttattagga aatccaagtg cggggattca aagcacttat gcgcgtctgg cgctaagtgg
     1561 tggattacgc catgacatgg gaggattaac gggggggagt aatagcgctg tgaatacttc
     1621 gaataaccca ccagcgccgg gatcccatcg tttcgtctaa atatatccat
//
LOCUS       DAZGXS010000334         1657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGXS010000334 DAZGXS010000000
VERSION     DAZGXS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..341)
                     /locus_tag="ACHKG5_005049"
     CDS             complement(<1..341)
                     /locus_tag="ACHKG5_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP0870949.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKF"
     gene            complement(350..1117)
                     /gene="sctT"
                     /locus_tag="ACHKG5_005050"
                     /pseudo
     CDS             complement(350..1117)
                     /gene="sctT"
                     /locus_tag="ACHKG5_005050"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1119..1379)
                     /locus_tag="ACHKG5_005051"
     CDS             complement(1119..1379)
                     /locus_tag="ACHKG5_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0870950.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1390..>1657)
                     /locus_tag="ACHKG5_005052"
     CDS             complement(1390..>1657)
                     /locus_tag="ACHKG5_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0870951.1"
                     /translation="DNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLT
                     LGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          580 a          338 c          278 g          461 t
ORIGIN      
        1 tcaaacttta attttacagc ttcagttgct aattgcattt tactttgaaa ccaggagaca
       61 agtattgttg tgaacatgca aaccagcaaa aaaggaatga tcgttttcaa ccaaccaatc
      121 cccatagctt taaaatagtc ccaaatatcc ggtttagcgt tatgatcaag gatatattca
      181 agaatcgaca taatgtgatg cacatcaaaa acatatccga gatataaagt acccacaagc
      241 attattacag aaacggttaa gtctctactt tttagaattt gccctttttt agaagcatcc
      301 tgcagtttct tttgtgttgg cttttcagtt ttatttgcca tgaaacactc tatttctcaa
      361 tgtatagttg caattgttct gggaaaaagg ataacggcat cactcgttca gccagtattg
      421 ggccaaaata aataattaat attaaaaatg ccagcgcact tttgacagtc agtgaaatgg
      481 caaaagcatt tagttgtgaa gcatagcgtg ccagcaatcc caggacagcc tcaccaccca
      541 gcataacggc aattacgggg cttgcgtaaa cgatagtatg agtcattata ttattaatat
      601 atgaaaataa aggctctaat ttaggaaaat taaaacttcc tgaaggccat aaattataac
      661 tttgctaaag agtttccaga ataaagttca atccaccgtt agtaagataa accgctgcag
      721 agaaaaggtt aaataatctt gctaattccg aggtatcgac acctgtagcc ggatccaaag
      781 tactacttaa cgttgcaccg cgttggttat cgataaagct acctatagca aggaaaatcc
      841 aaaatggaga tgctaataag caagcaatga acagccctat gataatttct cttgcagcta
      901 acaaagcgat atcgatttca tttaaggtcg ataattcata gtggtaatgc tgaactaatg
      961 cacatgatac cactattatt attggatgac ggactactgt tggtatattc ccactggcga
     1021 aaaatggtaa caaatagaaa gttggtgcca atcggcaaaa accgagcgct gtagcagcca
     1081 gtagactatg caactgatag agtatcacct cccccatgct atcctctcgc aaatgctaaa
     1141 ttcagcattt caataccaaa agaatataat ttttcaccat accatccgct cattaaaaaa
     1201 aagcaaatac ttacgcacaa taatttcacg ccaaatggca acgtctgttc ttgcaactgc
     1261 gttaccgttt gaaaaagccc taccaataaa ccaacaaatg tcgctactgc aataggtcct
     1321 gcagacataa ctaaaatcag atacagcgcc ctattccctg caaaaacaat gtcatccata
     1381 tttttttcct cagggattaa tggataggtc aaaatattgt aatatgagtc cttttgataa
     1441 cattgtccaa ccgtccatcg cgacaaacag aattaatttt atcggtgtag atatggttac
     1501 cggactcatc atcatcatac ctaacgttaa cagaacgctg gatataacca gatccactac
     1561 aacaaagggt aaatagatat aaaaacctat aataaatgca ctttttatct cacttaacgc
     1621 atatgccggg agtaacgaga aaattgaaat attatca
//
LOCUS       DAZGXS010000335         1647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGXS010000335 DAZGXS010000000
VERSION     DAZGXS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..589
                     /locus_tag="ACHKG5_005053"
     CDS             <1..589
                     /locus_tag="ACHKG5_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0870952.1"
                     /translation="CAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPGSDDL
                     NGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAGPSVR
                     VNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAWGAGV
                     QFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            649..>1647
                     /locus_tag="ACHKG5_005054"
     CDS             649..>1647
                     /locus_tag="ACHKG5_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0870953.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDTGATGPAGPQGPKGETGAAGPVGATGPQG"
BASE COUNT          412 a          399 c          542 g          294 t
ORIGIN      
        1 ttgtgcagca attttgtccg cagccatttg tctggccgta tccggtgtgc ctgcatgggc
       61 gtctgagcat cagtccacgc tgagcgcagg atatcttcat gccagtacgg acgctcccgg
      121 cagcgatgat ctgaacggga ttaacgtgaa ataccgttat gagtttacgg acacactggg
      181 gctgattacg tccttcagtt atgccaacgc cgaagatgag caaaaaacgc attacagcga
      241 tacccgctgg catgaggatt acgtgcgtaa ccgctggttc agcgtgatgg cagggccgtc
      301 tgtgcgtgtg aatgaatggt tcagtgcgta tgcgatggcg ggtgtggctt acagccgtgt
      361 gtcgactttt tccggggatt atttccgcgt aactgacaac aagaggaaaa cgcacgacgt
      421 gctgaccgga agtgatgacg ctcgctacag caacacatct ctggcgtggg gggctggcgt
      481 gcagtttaac ccgaccgaat cagtggccgt tgatgtcgct tatgaaggct ccggcagcgg
      541 tgactggcgc actgacggtt tcatcgtggg tgtcggttat aaattttgat tagtaacaca
      601 gcgttatgac agcccgccgg ttcaggcggg cttttttgtg gagtgggtat ggcagcagta
      661 caaatatcag gcgtgctgaa agatggtgcg ggaaaaccaa tacagaactg caccattcaa
      721 ctgaaagcca gacgtaacag caccacggtg gtggtgaaca cggtggcctc tgaaaatccg
      781 gatgaggcag ggcgttacag catggacgtc gagtatggtc agtacagcgt cactctgttg
      841 gtggagggat tcccgccatc acatgccggg accatctccg tgtatgaaga ttctcaaccg
      901 ggtacgctga atgattttct cggtgccatg acggaggatg atgcccgtcc ggaggcactg
      961 cgtcgttttg aacagatggt ggaagaagca gcgcgtcacg cagaggaggc gaagaagaat
     1021 gccggagaag cagagacgtc cgcgaggaat gccggcatat cagccagtaa ggcggaagcg
     1081 agcgccgcaa atgctgatac ttcagcagag gatgcatcgg agtcagcccg gcaggcggca
     1141 gaaagtgcag cctctgcaaa gaagtcagag gaagcgtcct cgtcctcagc ctctgaggcc
     1201 gctcaaaaag ccagtgagtc attacaaagt gcaacagatg ccgagttgtc aaaaaagacg
     1261 gcagaaagtg cagccggtaa tgcagccagg gatgcaacga ccgcaacaga aaaagcccgg
     1321 gaatcagcag aaagcgcaca gtcagcggaa caaagcagaa tagcggcgga agacgccgta
     1381 aacagaattc ccaccgtggt ggggcctccc ggaccaaagg gggaaccggg tcccgcgggt
     1441 cctcaggggc cgaagggaga taaaggagag cgtggagaca ccggtccggc aggggcaacc
     1501 ggtgaacggg gaccgggagg agatacaggt ccggcaggtc cacaggggcc aaagggagat
     1561 accggggcaa caggtccggc aggaccgcag ggaccgaaag gggaaacagg tgcggctggc
     1621 ccggtggggg ctaccggacc tcagggg
//
LOCUS       DAZGXS010000336         1618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGXS010000336 DAZGXS010000000
VERSION     DAZGXS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            378..1322
                     /locus_tag="ACHKG5_005055"
     CDS             378..1322
                     /locus_tag="ACHKG5_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870954.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1404..>1618
                     /locus_tag="ACHKG5_005056"
     CDS             1404..>1618
                     /locus_tag="ACHKG5_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870955.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLL"
BASE COUNT          523 a          329 c          276 g          490 t
ORIGIN      
        1 tgcaacacaa ttattacaat gaaaaggtat agataaaaaa ccagtatttt atttaacaga
       61 accgccttat ccacttttta taataccaat tgattagagt agccaatttg ttgctatttt
      121 acataacata tttttatcaa agttaattga ttgttgcctt tatgagcacc gaccgtaagc
      181 ccgttatgtt attgtttcat tagtaataat cacttaaatg tatcgatgaa acaggtatga
      241 cttaccggga gtgattgttt ctgagtgaac agaggccact ttcgcctgat aaatgaaaaa
      301 tgagcgattt ttgcaagtta gtttttctat agccgagtac gctcagcgcc tcgcttgatt
      361 acaatgaggt tgctgctatg agtcctgata atatcggttc ggctgtcaat cgcttctcag
      421 gttggccatc taatgattct ctaaaaacag ataaagaatc aatgacatca cccgtgactg
      481 tgcaggtaat aaagatgatt gccgccatcc ttgacccaca tcggatagtc gttggaattg
      541 atacacttta tacaacgcag cgcgaaacat ttacctcaga gagtcaacag ctcattgaat
      601 cagcattgca aacacttcat gtgatgaatt taaataatct ggaaacaaat cgcgatgaag
      661 aaagtcacta tctggctttt gaatataacg ttgacacctc tgacagaata accgtttctg
      721 cttataatga aaaaaaacct ggctgctatg gccaatgtct tgtaatacag gatgacgaaa
      781 cagacttatt taacctgata aaaattaaaa ttttcgaagc caggcataat tatgtcgctc
      841 aattgatgag cgatccggaa tttatgttca aattcagtta tagcgctcaa caaaatagat
      901 tagaacctca tcttctggtt cccgcttatt tccctctaaa aacaaacgaa ccggttacgc
      961 aagaggatgt actgttactt tatcgtttct ttaaaatgaa tgatgatttt aataaattaa
     1021 catcagatga atacatgagt atcctgacac ctctgatgaa gtgtgagcgt agcgtccacg
     1081 acaacaacag gtatgtgacg ggcaaagata cccttcttct ggactatcct ccttctggta
     1141 accagataca tttccatgtt ttccctgacg aatcagcaac actagtcctt tatttttcaa
     1201 acaatgacat tgaatgtttc gtctttgaaa gagatattcc gagccagtat cgctttttca
     1261 aaatgtttac caatctggca ctggttatag atcagttaac cgaagccaat aaaatacttt
     1321 aattttctat atatccccta tggcaaatat tgctaaacaa tgcctgggga ttaattttta
     1381 ttactcacgg aaagatttaa ataatgatac catctcagat ctctttcaat acccttccag
     1441 tcaatgcaac atacgcttca gagacaacag ttgacacaca aaaattctct gacatcctct
     1501 actcagcggg gtgttcccta aaggatatgg cttatctgag cagatgtctg gcatcaatac
     1561 acccgactct cgccaaaaac atttatgaaa cagaaaatct cagcgatcaa aaattact
//
LOCUS       DAZGXS010000337         1537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGXS010000337 DAZGXS010000000
VERSION     DAZGXS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            261..>1537
                     /gene="espK"
                     /locus_tag="ACHKG5_005057"
     CDS             261..>1537
                     /gene="espK"
                     /locus_tag="ACHKG5_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP0870956.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGK"
BASE COUNT          540 a          324 c          280 g          393 t
ORIGIN      
        1 gagaacgttt tttcttctcc cttttagcaa atcgctataa aaaaacaaca aatggtataa
       61 aaatgagcaa tcctaatcca tgcatgacgg actggagaaa gtattcgcaa aagttaaaat
      121 caacagtctg ttggttttga gatataactc tcttttctaa tattgacacc ttggctttgc
      181 tggttgttaa aattagcttg ttgattagta gtatggatag aacttactct gttatttatt
      241 tctaaaaatg gaatttatca atgcttccta catcgcaatt acgaccgacc gggacattct
      301 gctcctattc cgctgaaaca tcagcagaca tcaaaagcga aatcacacca attcagatag
      361 aagaagcgcg ggccagtggt cgtttatata tcaaagattg tgatattgag tatctgccac
      421 agttaccaaa cgaaataaca tcagttacaa tcgaaaactg caacaacctg acaaccctta
      481 caggattgcc ggttaataca caaaacctct ccgtcattaa ctgtgaaaaa ttacaaatca
      541 cagacatgcc atcaaccgta aaaaatctac atattgaatt aactgattca ccatttatac
      601 atttcatatc tgaaggcatc gagtgcctga cggtttgcca ctgctatata tctggagtgc
      661 cagagagtgt ccgctacctt gaaataaaag gtagcgccac agacagcata aaaaatgttc
      721 caaacgggtt atcatctctc agcatcaata gctataaccc ggagaatcag gccagaattg
      781 acaacctgat atcaccgtca ctgaagacgc tatcgctgac tggatgtagc aatattatac
      841 tgccggagaa acttccggag agtgtgacat cggtaaccat tcatgcggag cagaaaacca
      901 cgtggaacat cggtgttgaa gggatgcctg atgggctgga tcttgattta caaaatgtac
      961 tactctctcc agatgtagtt aaagcaaaaa acatcacctt tcagggcaac gctctggatg
     1021 tggccttaca ctttcgcgag ggagacattg tctatggact atcttcaccc agagaaaaac
     1081 ttgtcaacag cattaaacta gttaacgact tttccaaaaa agatattata actcagaata
     1141 cgttaacaaa cgcagtatgg gaccccagaa cacctcgcaa atataagcaa gatccactta
     1201 tcaaaagagc attaaatgaa cacgaaagag ggataaaatt taaacaacac ttaaagaatc
     1261 acaataatta taatgttacc atggccgacc tttccgtata caatcgcgac aaattatggg
     1321 caaaaacaag caaggccggc ctagagtttc agacattaac acgcaataaa acggttattt
     1381 tttgtgcgga tgagcttgtc aactcactca aactcatagc taacaagtca gagggctatg
     1441 gccagagtat taccgccagc gaattacgat ggatttaccg taataaagac aacaaccaaa
     1501 taatgaaaaa cataaaattt tatctacatg gcaaaga
//
LOCUS       DAZGXS010000338         1483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGXS010000338 DAZGXS010000000
VERSION     DAZGXS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1122
                     /locus_tag="ACHKG5_005058"
     CDS             <1..1122
                     /locus_tag="ACHKG5_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0870957.1"
                     /translation="RLLKQQKEQLALVGQNTELAKLKYQTALGELKTLTEMQKQELLR
                     NATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDS
                     FRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERPKDQAVFYAESDVQRADWQKGL
                     QEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKV
                     LMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDT
                     PTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQN
                     DGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            1115..1456
                     /locus_tag="ACHKG5_005059"
     CDS             1115..1456
                     /locus_tag="ACHKG5_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0870958.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
BASE COUNT          356 a          336 c          514 g          277 t
ORIGIN      
        1 cggctgctga agcagcagaa agagcaactg gcactggtgg ggcagaatac agagctggcg
       61 aagctgaaat accagacagc gctgggtgaa ctgaaaaccc tgacggagat gcagaagcag
      121 gaactgctgc gtaacgcgac cctgattgac cagcaaaaaa tccgggaaca gttgcgatcc
      181 cgggaagaga cactgaagaa tgagaatgcg gctgcgcgtg cgtcgaatga tgctgaactg
      241 ctggggtacg ggcaggggga gcgagccaga gaacgcatgc gggagttgca gcagatccgc
      301 gacagcttcc gccagaagga tgcggacctt cagtctcagt atcagaccgg ggatatcagt
      361 gaggattttt acagacaggc tctggcacag aacgcgcagt atctgagcga acgccctaag
      421 gaccaggcag tcttttatgc cgaatcggat gtgcagcgtg cggactggca gaaagggctg
      481 caggagggat tcagtaactg ggtggataat gcgtccgatt acgcctcaca ggcagcacag
      541 ctggcgacgg agggtatctc agggatggtg aataacatca cggagatgct gaacggaaat
      601 aaagtggaat ggcgcagctg ggcctcatca gtgctgcagg aaatatcaaa agttcttatg
      661 aatgccgcga ttgtcaacgg aattaagacg gcggcaaacg gtatgtccgg tgcgggagga
      721 tttctcggca gcattggtga ctggctgggc ggagcggtgg ccaatgcaaa aggcggcgtg
      781 tatacctcgg caaacctgag cgcgtacagc aacagcattg tggacacgcc cacgtacttt
      841 gcgtttgcaa aaggggccgg gctgatgggg gaagccggac ctgaagctat tatgcccctg
      901 acccgggcgg cggatggctc gctgggcgta cgcgccgtgg gcagtatgaa cggcagtgct
      961 ggtctggtgt attccccggt ctaccacatc gccattcaga atgacggggc taacggacag
     1021 atagggccgg aggcggcagg cagtcttgtg cagctgattg accagcgggt gcaggcggtg
     1081 atgctgtcca tgcgacgtga cggaggaatg ctgagtggct gagataaaaa cgctgcatct
     1141 ggtcccgcgt gaagggatgc aggtgagtga gaagccgtcg gtggtgaggg tgcggtttgg
     1201 tgacggttat gaacagcgcc gccccacagg gctgaatcct caactgaaga cgtttcaggc
     1261 ggtgttccgg gtgacggatg agtcaacccg gcgctggctg gatgaatttt tatcctggca
     1321 tggtggttac cgtgcctttt tgtggcgacc gccgaaacat aaccggacgg tgagggtggt
     1381 gtgccgggag tggagcgtca cggataacgc ccggtacagt gatttcagct gtacgattga
     1441 gcaggtggtg aactgatgca ggacattcat gaagaaagtc tga
//
LOCUS       DAZGXS010000339         1460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGXS010000339 DAZGXS010000000
VERSION     DAZGXS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            297..458
                     /locus_tag="ACHKG5_005060"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHKG5_005060"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHKG5_005061"
     CDS             442..507
                     /locus_tag="ACHKG5_005061"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP0870959.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHKG5_005062"
     CDS             623..880
                     /locus_tag="ACHKG5_005062"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0870960.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..>1460)
                     /gene="nleF"
                     /locus_tag="ACHKG5_005063"
     CDS             complement(927..>1460)
                     /gene="nleF"
                     /locus_tag="ACHKG5_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFP0870961.1"
                     /translation="SWMYVSGRGNPSTPESVSELNHNHFLSPELQDKLDVMVSIYSCA
                     RNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGMTIEDRDSYIRDL
                     FFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCLVEGCSDDFNPFD
                     IIRVPDLTYNKGSLQCG"
BASE COUNT          434 a          339 c          292 g          395 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga
//
LOCUS       DAZGXS010000340         1458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGXS010000340 DAZGXS010000000
VERSION     DAZGXS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            625..816
                     /locus_tag="ACHKG5_005064"
     CDS             625..816
                     /locus_tag="ACHKG5_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870962.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(886..1026)
                     /gene="ykgO"
                     /locus_tag="ACHKG5_005065"
     CDS             complement(886..1026)
                     /gene="ykgO"
                     /locus_tag="ACHKG5_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0870963.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1026..1289)
                     /gene="ykgM"
                     /locus_tag="ACHKG5_005066"
     CDS             complement(1026..1289)
                     /gene="ykgM"
                     /locus_tag="ACHKG5_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0870964.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          444 a          287 c          312 g          415 t
ORIGIN      
        1 gtgtcaatat gtgaacagaa aatgaattcc atggttaagt caatatttac aaaaaatgaa
       61 aatattgctt ttttgataag aagaaaaaga cattcagttt cataaggtta ttggcaatat
      121 aacgttcaat tcttacttgc attttatatt agccaggaac tttgccctga ggtgttagga
      181 atagtcttaa tgtgttgtgt aatggactag taaaaaaaca agtctaaatg ataagaaata
      241 gaatatgaaa ctatcatgag tcataattaa agatgatttg ccaatcagta aggtgtttta
      301 atggattaac atgccgcaca aaatggttat gggtgttcat cccaataagt gatgaaaaat
      361 taaacaggtg taagtacgtc atctgtccgg taagccccct aagagccaga ttaagtgaag
      421 ttaaacgaag actgccagtt gtcaccggaa cgatggcctc tttgccaggg cactgcattc
      481 agtcatctgt gcgcgttact ctaactggca ctcgaacgtc ggcgacgggg ttaccggtgg
      541 atcaggccgt tcctgtggcg taaacgtttt tatgtcagta ccaacagatt taccacattt
      601 atcatcttaa cagtttgagt gtaaatgcag gctgcgtcgc aaagggagga aactgagtgg
      661 cgtgttcagt ctaaaagagg gttgatgcca gcatacaggg gcgaagctgg tcaacaagtc
      721 aacattaata taatggaata ttcggagaga aacgtcaggc agttgacctt aaatgaacag
      781 gaagatacat ccccaggaaa attaatgctg gtgtaataaa tacaccaaca ttaatccggt
      841 ctgattatta atcagacaaa tatcgttaat tcgtcgaatt ttaaatcaac gttttttctt
      901 acgaccctga acggccttaa agcgtggatt agatttacaa atcacatata gccgtccttt
      961 tcgcttcaca atctgacagt ctggatggcg ttcttttgcg gtacgcagag agttaaggac
     1021 tttcatcacg cccccttttt cgtgctaaca aaacgaccaa aacgttgggt gaatcgtgca
     1081 acatttcctt ctgatgccac tgttctcagc ttccctgtat agaacgggtg cgatttcgaa
     1141 gagacatcaa ttgttacgta cggatacgtt acgccatcca gctcaatctc acggtctgtt
     1201 ttgatagtcg agccgatttt aaagtactca tcaacactgg tgtcgtggaa caccacagta
     1261 cgatactcag gatggatatt gggcttcatc atttttacct gttatgttat aacataacca
     1321 caaagataca tgctcatccg gatcatttca acacccaatc cctgtgacca ttagtggtat
     1381 gtggaatttc ctcaacgggg aagtctggca ttgttaacag ccattggcga cgctcccata
     1441 aacgcctgga tgagtgat
//
LOCUS       DAZGXS010000341         1445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGXS010000341 DAZGXS010000000
VERSION     DAZGXS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(148..717)
                     /gene="ybfC"
                     /locus_tag="ACHKG5_005067"
     CDS             complement(148..717)
                     /gene="ybfC"
                     /locus_tag="ACHKG5_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP0870965.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
     gene            complement(714..>1445)
                     /locus_tag="ACHKG5_005068"
     CDS             complement(714..>1445)
                     /locus_tag="ACHKG5_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0870966.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          371 a          320 c          275 g          479 t
ORIGIN      
        1 aaaagttgtg ggtaaaacat tacattcaga atatatgata ttcttaccat cggaatccat
       61 ttttccaaat cgaccttctg gtgtacctgg atatccatta agaacatgtt tttccgaatg
      121 gttaatttat tttctcagga agatgtttta tttttcttca ctacgaagca gttcatgctg
      181 tatttttgtt aaacattcgt aagactcatc tttgttcggg agatgtgctg tcattcgagc
      241 ataagttggc ttctcatctt taaaatattt aaccatgatt gaattatcaa ggactgccaa
      301 caactgagct ggatcactgc tctttttttg atagataccc gctcttatat aacttctaaa
      361 ccgatcgccg agaaacgccg ttttttctgc tgtatttatc aactcctgca acgttccttt
      421 gatggcatga ttttcattaa ggcgtagtga cccgacacga attaagagtc tcgccccgct
      481 tggtgtgaat ttattatttt tacatgtacc cagttctgtt tgccaataat actcaccgat
      541 gtcctcattc tgataccatc tccacatggc tttattattt aagacaggaa acgcggaaaa
      601 aatagtaccc ttagcgaaaa tagtaaaaca atcatgatct ttagcttgta tttcgcaatc
      661 agctataaca ccaaaactaa caaatatcat cagcaaaaaa aacaaaactc ttttcatttt
      721 cttattcctc tcgatgttct ctgtttcatt ttagctaaaa tttcacccaa atcatctatc
      781 cctgctgttg ctggcccatc gggtttatta atgtcaggga ataaataggg ttctgcacaa
      841 gtatatcctg gtggtaattg agttgttggc caatttgtct gtggcaggta atcacttgct
      901 gtctttgtat ccatccctgg atactgggga tcatcctgtg gcgtacaagt cagatttggt
      961 tttacccact tcctgcaata cagtgtgtag tcgtaagggc ttaatgctct gaagaatgtt
     1021 ttgaaaacat taggcgcact actggcctgg tcaataggat atggcagtct taattgtttc
     1081 gctatgtctg gaaatttact tggatcgggc gtaggcggtt tgttgggtct cgctccatat
     1141 ccgccattat ttaagtgtcc atattttgat gccagtccca ttgaatctat atactgaacc
     1201 ggattcaacg gatactgata aaaattccat ccccccttca gtccaatcgg atcctgagtg
     1261 atatacctcc cctgcagcgg gtcataatag cggtggcggt tgtaatacag gccggactcc
     1321 tcatcatact gctgccccgg caggcggata agctgctgca gctgatgcgg gttctcttca
     1381 ttcagcaggt tgccccactc atcgtattct gcgcaccatt ctgttgcccc ttccttactg
     1441 ataag
//
LOCUS       DAZGXS010000342         1437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGXS010000342 DAZGXS010000000
VERSION     DAZGXS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..517
                     /locus_tag="ACHKG5_005069"
     CDS             <1..517
                     /locus_tag="ACHKG5_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0870967.1"
                     /translation="YFNVFKRHGYINEDGSPYLLRTHQLRHLLNTFAQINGMDEFSIA
                     RWSGRKLISQNVSYDHRSHLQMSKAIREKKLSVCVNEHRIKDIPVVDLNEFDSLSSGA
                     VLVSKHGYCKHSYAFKPCDNYPIKNSGLDNETISNIHDKILKRTLYDKNDGNINADKW
                     YEFHKKIKKGE"
     gene            518..917
                     /locus_tag="ACHKG5_005070"
                     /pseudo
     CDS             518..917
                     /locus_tag="ACHKG5_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1015..1428
                     /locus_tag="ACHKG5_005071"
     CDS             1015..1428
                     /locus_tag="ACHKG5_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870968.1"
                     /translation="MDKTNINLMERYLLLLDRFVDKLAESGFSEQRIIEQSYLFCAGF
                     YIKYQSGIEKMTFSNREVVLTFLLLSYYSHINKLDDDLINKERMKHVCSSLINFIVSN
                     GSRTEKVYANEKRKYEASTLKRELSKKDKRKRYRL"
BASE COUNT          536 a          200 c          266 g          435 t
ORIGIN      
        1 gtattttaat gtttttaaaa gacatggtta tataaatgaa gatggtagcc catatttgct
       61 aagaacacat caactaaggc atcttctcaa cacatttgct caaataaatg gtatggatga
      121 atttagtatt gctcgctggt ctggacgtaa gcttatttct caaaatgttt cttatgacca
      181 cagatcgcat cttcaaatgt ctaaagcaat aagagaaaaa aagttatcag tatgtgttaa
      241 tgagcacaga ataaaggata ttccagtagt ggatcttaat gagtttgact cacttagtag
      301 tggtgcagta cttgtatcaa aacatggcta ctgcaagcac tcatatgcgt ttaagccgtg
      361 tgataattat ccaattaaga actctggttt agataacgaa acgatttcaa atatccacga
      421 taaaatttta aaaagaacac tgtatgataa aaatgatggg aacataaatg ctgataaatg
      481 gtatgaattc cataaaaaaa taaaaaaagg agaataaatg gctaagcata taaatggtta
      541 tcatgagaag aagattgtag atgttattaa cacatggtct atagatgaaa aactaacctg
      601 gaatgctttg tgcgataggt tagtcagggt tattgggaaa agaccatcaa gacaatcctt
      661 aagtagtcat gtaaggattg cggaaagctt caatgtgaaa aaaaccacaa tcaaatctgg
      721 tgtaatacac actgtaaaac ctgctaattt aaaagtggct tctcaacgaa ttaaaagact
      781 tgaagcagaa aatgaagctt tgaaggcatt aaatgaacgc tatttagagc agttcaaagt
      841 atggcattta taatgctcac cttaaacaaa taacgattga agaacttaat aatccgttac
      901 ctgcaaaata cctttaataa aattgatagg ttttagctat tgacatggtg aaatattatg
      961 ttatttattt tcttgtcagg acgacaaaat ttcactattg caaggatgca tattatggac
     1021 aaaacaaata ttaacttaat ggaaaggtat cttcttctgc ttgatcgatt cgttgataaa
     1081 ctcgctgaat caggtttctc agagcaacga attatcgaac aatcatatct tttttgtgca
     1141 ggattttata tcaaatacca gagtggtata gaaaaaatga ccttctccaa cagggaggtt
     1201 gttttaacct ttctgctact ttcatattat agtcatataa ataaactgga tgatgatttg
     1261 atcaacaaag agcgtatgaa gcatgtatgt tcttcattga ttaactttat agtcagtaat
     1321 ggtagccgaa cagagaaagt ctatgctaat gagaaaagga aatatgaggc ttcaactcta
     1381 aaaagagaat tatcgaaaaa ggataaaagg aaaagatata ggttgtagtg tttgtat
//
LOCUS       DAZGXS010000343         1435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGXS010000343 DAZGXS010000000
VERSION     DAZGXS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(444..>1435)
                     /gene="nleL"
                     /locus_tag="ACHKG5_005072"
     CDS             complement(444..>1435)
                     /gene="nleL"
                     /locus_tag="ACHKG5_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP0870969.1"
                     /translation="LQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEV
                     YLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVD
                     MLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILD
                     ILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGS
                     HVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQ
                     VFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          522 a          312 c          189 g          412 t
ORIGIN      
        1 gatttgccca gaaataacga acataatttt cgttgtaatt aaaatcatat ttcatctata
       61 gtgaccattc acagcaaaac aaaatcaatt atcacattca aaaaaatact gacaacaaag
      121 atactcttca gacaaagttg tcatatgtaa attttccctt aaaacgttgc tcaagaaaaa
      181 tataaaaatc atatgcccta atcaacatcc tcaagcacta cgatatatgc agtatcctat
      241 tcatatatta ccaccatatc agacaaatcg aatcacaacg agttctatcg tactctttca
      301 tcttcgacac tgtaaatttt aagaacattc ggctcatgcc actaaaccaa gacataataa
      361 caacggagca ataaatgttc ccactgaaca tgtgcaaacc taaaaagaca gtgttacaat
      421 aatattcatc catggtctct aaatcaacgc cacgcaacag gataatacaa tgaaaatatt
      481 tcaggaaaag attcttttgc atgctccgtt agcatatcag tcagcacagc ggtacaagta
      541 aaaacattat ttcttccttg tagcctgtct aaaatatcat tgtaataata aactgagcca
      601 aatacctgag gatctgtttt ataagcttgt tcaatcaatc catttgcata tctccgtaga
      661 atctcaggag aatcatactc tgtaccaaac atatcactcg atgagtattt acagaaaacc
      721 ccccctaaac aaaataatat ttcagcctgt tttcttagtg gcatatcttt caatacatgg
      781 ctccccataa tcatatctac atgctgagcg ttcagttccc aaccatcaag aaagggattc
      841 cagacacagg caagtttcct ttgctgatca tcagcaatca ttttataatc tgatttattg
      901 gaattaagtg cctcaataaa tctctctttg agttcattat cagaaatgag tatatccaga
      961 ataccagaaa cgaaattctg ggaaaaagct ttatgataag gaatactgaa aattggaaat
     1021 gattcagaaa aaagattgct caatgaatac ccaaatgtat tttgaacttc tccatcctta
     1081 tataaaaaaa aggacatcca attagttgat gtattaggag ataacatatc aaccatatga
     1141 ctcaaagaac acatcattgc cataccatca tcctgtgatg ataacaaaac ccagttataa
     1201 gccaaatcgg aatcatcatc ccagtcaggc ttcccagagc catcgcacaa tccaaaatca
     1261 ctctgctgtg tgacaggata aactttttca ttttttaaat atacttcata gagttcccta
     1321 aatcgttcct gcattccatc acgccgtgaa ccattcatta caatttgtat aaaggaacta
     1381 ttataagata ccatcgcacc tggatgtcgc tcaaaataca tccctgcctg caata
//
LOCUS       DAZGXS010000344         1423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGXS010000344 DAZGXS010000000
VERSION     DAZGXS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            63..626
                     /locus_tag="ACHKG5_005073"
     CDS             63..626
                     /locus_tag="ACHKG5_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0870970.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREVAAIPQPDENNPEMPPPAVIS
                     HGIEYMEDGLPDPVKAMGQILVENLIIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNQNEESG"
     gene            623..>1423
                     /locus_tag="ACHKG5_005074"
     CDS             623..>1423
                     /locus_tag="ACHKG5_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0870971.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGA"
BASE COUNT          346 a          360 c          439 g          278 t
ORIGIN      
        1 acgccgggaa aaatgggctg aaccataccc ggctgagtgc gttatggacc cgggaggagg
       61 ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg aaaaacaaaa
      121 cagcgtcagc cattgccgcc ggttattcgg cgaagaccgc cagggttaaa ggctcgcagc
      181 tggcaaaaga tccggaggtg ctcgcgttta tagcccgtaa acagtgcgaa acggtggagg
      241 tggatgaggt tcctgtttac cggcagaaaa aatcagagca ggaggataaa ccccgtcgcc
      301 gtgaggtggc tgcaatacca cagccggacg aaaacaatcc ggagatgcct ccgcccgcgg
      361 tgatatctca tggtattgaa tatatggagg atggtcttcc cgatccggtg aaagctatgg
      421 ggcagatcct ggtggaaaac ctgataattg acccgaaact ggcactggat gcggcctggc
      481 gactggcgca gttcacacac cataaaaaag gcgatgccgg taaaaaatcg gcaaaaggtg
      541 atgccgcgaa aaaagcggct aaccgttttg cggtgccacc acctccccgg ctggtggtga
      601 ataaccagaa tgaggaaagc ggatgatacc tgtatggagc acggcatgtc cggactgggc
      661 agagcgcctg aaaaaggggc tgtcgattat tccggctccg atttatccgg accaggctgc
      721 acatgcactg gcgattttta aacaactgcg aattgtggat gcaccgggta gcccgacatt
      781 cggggagtcc tgtgcaccgt gggtgtttga cctggtggcg gccctgtttg gctcctacga
      841 tgcgcagacc ggtgttcgcc atatcaagga agtgtttatc cttatcccca agaaaaacag
      901 taagtccacg ctggccgcgg ggatcatgat gacggcgctg ttactgaact ggcggcaggc
      961 ggcgggctac accattctgg ccccgaccgt ggaggtggcg gctaacgcct tcaaccctgc
     1021 cagggatatg gtacgacggg acgatgatct ggatgacctc tgtcaggtgc agacacatat
     1081 ccggaccatc acccatcggg tgacggacac caccctgaag gtggtggcag ccgatccgaa
     1141 tacggtgtcc ggtatcaagt ccgtggggac actgattgat gaactgtggc tgtttggcaa
     1201 gcagtacaaa gcggaagaca tgctacgtga agccatcggc gggctggctt cacgtccgga
     1261 agggtttgtg gtgtacacaa ccacccagtc gaatgaaccg cctgccgggg tgttcaggca
     1321 gaaactgcag tacgcccggg atgtccgtga cggcaaaatt catgatccgc actttctgcc
     1381 ggtgattttt gagcatcctc ctgaaatggt ggaaagcggg gct
//
LOCUS       DAZGXS010000345         1422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGXS010000345 DAZGXS010000000
VERSION     DAZGXS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            45..1421
                     /gene="ygeH"
                     /locus_tag="ACHKG5_005075"
     CDS             45..1421
                     /gene="ygeH"
                     /locus_tag="ACHKG5_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP0870972.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFNPDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDE
                     LHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          517 a          244 c          246 g          415 t
ORIGIN      
        1 ttatgcagga tgcaagaaac caattttttc atagaggtta actaatggac ttagaaaata
       61 aattctcata tcattttctt gagggattaa cgctcacgga agatggaatt cttactcaag
      121 gaaatgagca agtttatatt ccaccgaaag agttaggtgt attaatagta ttacttgaat
      181 ccgctggtca tgtcgtactg aaagatatga tcatcgaatc agtatggaaa aatattattg
      241 ttagtgatga gtccctgaca agatgtatct attctttgcg ctgcattttt gaaaaaattg
      301 gctatgatcg ttgcatagaa acaatctacc ggaaaggtta tcgtttcagt gggcaggttt
      361 tcaaaactaa aataaatgaa gataacactt cagactattc tatcgctata ttccctttca
      421 ctacttcatt gaaaacactg gatccattaa tacttaatca ggaattagta caaatcattt
      481 caaataaaaa aatcgatggc ctctatacct atccgatggc tgcgacaaat ttttgtaatg
      541 atcacatatc gcaaaattca tttttgagaa gattcaatcc agattatttc gttacaggaa
      601 gaataaacca gaataatgca gtgaacactt tatacattga gttgaccgac gctaaaaacc
      661 ttttcctcat cgccagtaat catctacctg ttgatgaact acataataca tcacaattta
      721 ttatagaaaa tatccttcaa acagtacata atccacaacg atctgtaaga ttaactaagc
      781 aggaccaagg atataagaat cattatttat cagatgaaat gttagccgga aagaaagaac
      841 tttacgagtt cacccctgaa agcatttaca gggccatgac tatatttgat ggattacaaa
      901 ataaaagtga tatacagacg ctaaaaacag aatgttattg ccttctagcg gaatgccata
      961 tgtctttggc acttcatgga aaaagtgaac ttgaacttgc tgctcaaaaa gcattagagc
     1021 ttttagatta tgtatcagac ataaccactg tcgatggaaa aattttagct attatgggac
     1081 tgataactgg tctgtctgga caagcaaaag tatctcatat cttatttgaa caggctaaga
     1141 tacactcaac tgatatagcc tctctctact actatagggc acttgtcaac tttcataatg
     1201 aaaaaattga agaggcaagg atttgtatag acaaatcact acaactcgaa cccagaagac
     1261 gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt gcctaacccg ctcaaaaaaa
     1321 acatgaaact ctactataaa gaaactgaga gtggaagcca tcgagttata attgacaaca
     1381 ttttgaaatt aaagcagctg acgagaattt gtatgcgata aa
//
LOCUS       DAZGXS010000346         1411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGXS010000346 DAZGXS010000000
VERSION     DAZGXS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..137)
                     /locus_tag="ACHKG5_005076"
     CDS             complement(<1..137)
                     /locus_tag="ACHKG5_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870973.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VS"
     gene            complement(134..580)
                     /locus_tag="ACHKG5_005077"
     CDS             complement(134..580)
                     /locus_tag="ACHKG5_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0870974.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(577..927)
                     /locus_tag="ACHKG5_005078"
     CDS             complement(577..927)
                     /locus_tag="ACHKG5_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0870975.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(937..1263)
                     /locus_tag="ACHKG5_005079"
     CDS             complement(937..1263)
                     /locus_tag="ACHKG5_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0870976.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1260..>1411)
                     /locus_tag="ACHKG5_005080"
     CDS             complement(1260..>1411)
                     /locus_tag="ACHKG5_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0870977.1"
                     /translation="GETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRD
                     AGGGK"
BASE COUNT          301 a          491 c          331 g          288 t
ORIGIN      
        1 gacaccgatg aaatcaggct gaaaaccacc cacggaagcg ccaccgacgg cctgccatcc
       61 agcaggggga ccacatacgg gtacacctgc ccgccggcaa gatgcgccag atgaggatac
      121 aaatccgcct ccgtcatcgt ctcagtacct catcaatggc ccggttcatc cgagcaatcg
      181 ccacctgtgc cgcctgttca ctgcgcacat caaatgccgg gcgcacaaac gggtgcggtg
      241 gcatattcac ggtccccatt tccacaaacc gccagtagaa agcattgcgc gggttatccg
      301 ccttcatggt gttatcgctg ttaccggtgt ccggattaac accccggata tgcacaccgg
      361 attccatccc gccatcgcgg gagcaccggg aaaggaccac cacattgcgg cgaagttttc
      421 ccctgcgtac cggtgcccgt gacaccactt cttctttcag cacattcgca cccgcacggg
      481 ttgcctcacg cagcacccgg ttattttccg caccactcag aagctgcaaa tcgcggctga
      541 tgtcctccag ccccgaaaaa tccagcaggg tttcgatcat ttttcccctc ccagccgaca
      601 gagaatttcc agacgcccgc cggttgcatc cggaaccggg accccgacga cattcaggac
      661 atggtcacgc cagggaccac tcagcacatg aagtcgtgac gccgccgtga tttcacgacc
      721 agactgaccg cgtacccaga tgcggatttc cgcctgcgcc atttccgcac cggactgcat
      781 ccgctcccgg ctgctcctgc cccggatatc cgcatgaatt ttcccgcatg acacccattc
      841 ttccgtcatt tctccggcag cattacgggt taacaccggg ttcagaacac tgatcatctg
      901 tgtcagacga cctgcagata ttgccattcc ccctcctcat aacaccgtcg gacaacgcaa
      961 atcgtaaatc agcacggaaa cagaaaacgg cagttccccc tgcacgaggt cttcccgctc
     1021 agcaagatcc ggattccggt acagcatccc ggtcagtcgc atggcagccc ccttcatccg
     1081 ggttaatgcc tcaccaggga tcagctcacc gtcctcacta atcactttat cccggctgcc
     1141 ctggatgtag gccagcagca cggcggtagc ctgacgaacc ttgtccatca gcatctcatc
     1201 atccgcgtca tggtcgacac gcagatgtgc cttgatttct tccagtgtca gtaatgctgt
     1261 cactttccac ctcctgcatc ccgcccacgt tttgcagcca gggtccagcc tgatgaatga
     1321 gcttctccgg gcttatcacc ggtcatactg ttgcagtgcc acaacgagcc cccccacgtc
     1381 accgtatcgc cggggtggta agtttcgccg a
//
LOCUS       DAZGXS010000347         1409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGXS010000347 DAZGXS010000000
VERSION     DAZGXS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            83..1288
                     /locus_tag="ACHKG5_005081"
     CDS             83..1288
                     /locus_tag="ACHKG5_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0870978.1"
                     /translation="MGYKVKKFIMSSGERGCLILDKKSNLPTYYQNLFLTTDIRNRGA
                     TASTMEIVATNLLIFSNFLDGRGIDIIERVELKKYLSVAEIDDLVRYAKQRFDRQKII
                     NIKSTNYRFIAKRTFSYRIHVFSRYLKWLCGLVHSSKGIHAKYEVDVFIESIRAHIPR
                     NSSLNMNEISEKSLNEEEIKVLFRLLEIGGIENPFHKEVQVRNRLIFTLLLNLGLRAG
                     ELLNLKIDDFDLRDNTLSVVRRHDSKEDGRSYQPLVKTGERVIPLSDELAREIFDYIS
                     NSREKMTKRKKHNFLFVAHCTGKNAGEPLSISAYEKVISTLKRASPELYNLSGHRLRH
                     SWNYMYSKGIEGAELEYNRRKDLRNYLMGWSKESIMCDRYNYKYISQQEKDVVLKVYK
                     SVSKIISGV"
     gene            1290..>1409
                     /locus_tag="ACHKG5_005082"
     CDS             1290..>1409
                     /locus_tag="ACHKG5_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870979.1"
                     /translation="MENKCIESEQIFFAKMNRYSFKLSDKKWQLDKENCVYPHK"
BASE COUNT          540 a          176 c          289 g          404 t
ORIGIN      
        1 attgtaaaaa atttgaatta ttttgaattg gaatatattt aataagtgag agtgatatta
       61 atttgttaag tttgaggtga aaatgggata taaagtaaag aaattcatca tgagtagtgg
      121 agagcgagga tgcctgatcc ttgataaaaa aagtaatcta ccaacttact atcaaaatct
      181 atttcttact acagacataa gaaatagagg cgcaacggcc tcaacgatgg aaatagttgc
      241 aactaactta ctaattttca gtaatttctt ggatggtagg ggaattgata taattgagag
      301 agttgaactt aaaaaatatc tcagcgttgc agaaatagat gatctggtaa gatatgcgaa
      361 acaaagattc gacagacaaa aaatcataaa tataaaatcg acaaattata ggtttattgc
      421 gaagagaacc tttagttata gaatacacgt tttttcacgc taccttaagt ggttatgtgg
      481 attagttcat tcgtcaaagg ggattcatgc aaagtatgaa gtagacgtat ttattgaaag
      541 tatcagagca catattccaa gaaacagttc attgaatatg aatgaaatat ctgaaaaatc
      601 gttgaatgaa gaagagatca aagtgctttt ccgtctattg gaaattggcg ggattgaaaa
      661 ccctttccat aaggaagtac aggtcaggaa cagactaata tttaccttgc tcttaaattt
      721 aggtttaagg gctggagagt tgctcaatct taaaatagat gattttgatc tcagagacaa
      781 cactttaagt gtagtcagaa gacatgattc aaaagaagat ggaagatctt atcaaccatt
      841 agtaaagact ggagaaaggg ttattccgtt atctgatgag ttagcccgtg aaatttttga
      901 ttacataagc aatagcagag aaaaaatgac aaaaaggaaa aagcataatt tcttatttgt
      961 ggctcattgc accggaaaaa acgcaggaga acccttaagc atttcagctt atgagaaggt
     1021 gatatcaaca ctgaaacgag caagtccaga gctatataat ctttcaggcc ataggttaag
     1081 gcactcttgg aattatatgt attctaaagg aatagaaggt gctgaattag aatataatag
     1141 aaggaaagat ttaaggaatt atttaatggg ttggtcaaag gagtcaataa tgtgtgatag
     1201 atacaactat aaatatatat cgcaacaaga gaaggatgtc gtcctaaagg tctataaatc
     1261 agtaagtaaa ataattagtg gagtttagta tggaaaacaa atgtattgaa agtgagcaaa
     1321 tcttttttgc taagatgaac aggtatagtt tcaaactgtc agataagaaa tggcaactgg
     1381 ataaagaaaa ctgcgtatac cctcataaa
//
LOCUS       DAZGXS010000348         1387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGXS010000348 DAZGXS010000000
VERSION     DAZGXS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(168..578)
                     /gene="ybcV"
                     /locus_tag="ACHKG5_005083"
     CDS             complement(168..578)
                     /gene="ybcV"
                     /locus_tag="ACHKG5_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0870980.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSKLKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            864..1070
                     /gene="ybcW"
                     /locus_tag="ACHKG5_005084"
     CDS             864..1070
                     /gene="ybcW"
                     /locus_tag="ACHKG5_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFP0870981.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(1235..>1387)
                     /locus_tag="ACHKG5_005085"
     CDS             complement(1235..>1387)
                     /locus_tag="ACHKG5_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0870982.1"
                     /translation="IRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLTSEKRAYTS
                     IKSDEE"
BASE COUNT          401 a          255 c          291 g          440 t
ORIGIN      
        1 tcgtcactgt attcccggat taactatgtc cgcagccctg acagggaaac tcctctgcgg
       61 gagtgtccgg gaataattaa taacgatgca cacagggttt agcgcgtaca cgtattgcat
      121 tatgccaacg ccccggtgct gacacggaag aaaccggacg tgatgattta gcgtggaaag
      181 atttgtgtag tgttctgaat gctctcagta aatagtaatg aattatcaaa ggcatagtaa
      241 tatcttttat gttcgtggat atttgtaacc catcggaaaa ctcctgcttt agcaagattt
      301 tccctgtatt gctgaaatgt gatttctctt gatttcaacc tatcatagga cgtttctata
      361 agatgcgtat ttcttgagaa tttaacattc acaacctttt taagtccttt tattaacaca
      421 gtgttatcgt tttctaacac aatgtgaata ttatctgtgg ctagatagta aatataatgt
      481 gagacattgt gacgttttag tttagaataa aacaattcac agtttaaatc ttttcgcact
      541 tgatcgaata tttctttaaa aatggcaacc tgagccattg gtaaaacctt ccatgtgata
      601 cgagggcgcg tagtttgcat tatcgttttt atcgtttcaa tctggtctga cctctttgtg
      661 ttttgttgat gatttatgtc aaatattagg aatgttttca attaatagta ttggttgcgt
      721 aacaaagtgc ggtcctgctg gcattctgga gggaaataca accgacagat gtatgtaagg
      781 ccaacgtgct caaatcttca tacagaaaga tttgaagtaa tattttaacc gctagatgaa
      841 gagcaagcgc atggagcgac aaaatgaata aagaacaatc tgctgatgat ccctccgtgg
      901 atctgattcg tgtaaaaaat atgcttaata gcaccatttc tatgagttac cctgatgttg
      961 taattgcatg tatagaacat aaggtgtctc tggaagcatt cagggcaatt gaggcagcgt
     1021 tggtgaagca cgataataat atgaaggatt attccctggt ggttgactga tcaccataac
     1081 tgctaatcat tcaaactact taacctgtga cagagccaac acgcagtctg tcactgtcag
     1141 gaaagtggta aaactgcaac tcaattactg caatgccctc gtaattaagt gaatttacaa
     1201 tatcgtcctg ttcggaggga agaacgcggg atgttcattc ttcatcactt ttaattgatg
     1261 tatatgctct cttttctgac gttagcctcc gacggcaggc ttcaatgacc caggctgaga
     1321 aatttccgga ccctttttga tcaagagcga tgttaatttg ttcaatcatt tggttaggaa
     1381 agcggat
//
LOCUS       DAZGXS010000349         1387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGXS010000349 DAZGXS010000000
VERSION     DAZGXS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1319)
                     /locus_tag="ACHKG5_005086"
     CDS             complement(<1..1319)
                     /locus_tag="ACHKG5_005086"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0870983.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLE"
BASE COUNT          331 a          380 c          364 g          312 t
ORIGIN      
        1 tcttccagca acagttcagc aaagtccatt tcgaagccgc gacgacacat aatgcgcatc
       61 accacgatgt cggtggcttc accgccgaga gtgaaggccg gaacctgcca gccgcgcaga
      121 cgcagacgtt cagagaggtc gtacagggtg tatcccggat cttcaccctc tttcagtttg
      181 aagcaaaccg ccgggatgcc ttcgtccgga cgacccgtac agatgaactc atacggcccc
      241 agtttggcga tttcatccgc cagataagcg gcaacctggt aagaggcgtt ctgtactttg
      301 gtatagcctt cacgaccgag gcgcaggaat tcataatact gcgcaatcac ctgacccgcc
      361 gggcgggaga agttgatggc gaaagtacca atctgaccac cgaggtagtc aacgttgaac
      421 accagttcct gcggcagcgc ttcttcatca cgccagatca cccagccgca gcccagcgga
      481 gccagaccga atttatggcc tgaagcactg atcgatttca cacgcggcag gcggaagtcc
      541 cagacgatat ccggggcgac gaacggtgcc aggaagccac cgctggcagc gtcgatgtgc
      601 atgtcgatgt cgataccggt gtcggcctgg aatttatcca gtgcatcgtg cagcggttgc
      661 gggaactcat agttaccggt gtaggtcacg ccgaaagtcg gcaccacgcc gatggtgttt
      721 tcgtcgcagg cttcaatcat gcgtttcggg tccataaaca actgaccggg gcgcataggg
      781 atctcgcgca gctccacatc ccagtagcga gcgaatttat gccagcagat ttgcaccgga
      841 ccgcacacca ggtttggttt atccgttggt ttgcctgcag cttccatacg cttgcgccaa
      901 cgccatttca tcgccatccc gccaagcata caagcctcgg aagaaccaat ggtgttggtg
      961 ccaacggcct gaccattttt cggcgcaggc gcatgccaca gatcggcaac catgtttacg
     1021 caacgcaggt cgatggctgc ggattgcgga tattcttctt tgtcgatcca gtttttattg
     1081 atcgacaaat ccatcaattt atggacgttt tcgtcgtccc aggtctggca gaaagtggcc
     1141 aggttctgac gagcgttgcc atcaagatat aattcatcat tgataatctg gaatgcgaca
     1201 tcatcgcgca tttcgtgcag cggaaatcgt tttgactccg cgatagtaga aatggccttt
     1261 gcgccaaaac gtgaatcgag tagttctgag cggaaatccg ttaacagctt ctggtccatt
     1321 tcgaactcct taaatttatt tgaaggcaat aaaaaagtag gatttatccg caatggaagc
     1381 aaggcat
//
LOCUS       DAZGXS010000350         1385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGXS010000350 DAZGXS010000000
VERSION     DAZGXS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            153..>1385
                     /gene="pitB"
                     /locus_tag="ACHKG5_005087"
     CDS             153..>1385
                     /gene="pitB"
                     /locus_tag="ACHKG5_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP0870984.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGE"
BASE COUNT          317 a          330 c          359 g          379 t
ORIGIN      
        1 tatttctaaa gatttttaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc cacaccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttacttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcagc
      301 cacagcttgc cgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt tgccaaccga tttgttgctg aatatggggt
      421 caacccacgg cctggcgatg gtcttttcca tgctgctggc ggcgattatc tggaacctgg
      481 gaacgtggtt ctttggttta ccggcctcca gttcgcatac cttgattggc gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggaggcct gatattcctg ctgcgacgct actggagcgg aacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaagccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 gacaaaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gttaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc
     1021 cattgcctgc ggcatctact gatggcgcgc aagtgacgga gtttcactgt catccgggaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctctgata
     1201 cttccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcag
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgttgcg atgaccatcg
     1381 gtgag
//
LOCUS       DAZGXS010000351         1380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGXS010000351 DAZGXS010000000
VERSION     DAZGXS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..134)
                     /locus_tag="ACHKG5_005088"
     CDS             complement(<1..134)
                     /locus_tag="ACHKG5_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870985.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     V"
     gene            complement(131..577)
                     /locus_tag="ACHKG5_005089"
     CDS             complement(131..577)
                     /locus_tag="ACHKG5_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0870986.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(574..924)
                     /locus_tag="ACHKG5_005090"
     CDS             complement(574..924)
                     /locus_tag="ACHKG5_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0870987.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(934..1260)
                     /locus_tag="ACHKG5_005091"
     CDS             complement(934..1260)
                     /locus_tag="ACHKG5_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0870988.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1263..>1380)
                     /locus_tag="ACHKG5_005092"
     CDS             complement(1263..>1380)
                     /locus_tag="ACHKG5_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0870989.1"
                     /translation="VTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
BASE COUNT          287 a          483 c          325 g          285 t
ORIGIN      
        1 accgatgaaa tcaggctgaa aaccacccac ggaagcgcca ccgacggcct gccatccagc
       61 agggggacca catacgggta cacctgcccg ccggcaagat gcgccagatg aggatacaaa
      121 tccgcctccg tcatcgtctc agtacctcat caatggcccg gttcatccgc gcaatcgcca
      181 cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg cacaaacggg tgcggtggca
      241 tattcacggt ccccatttcc acaaaccgcc agtagaaagc gttgcgcggg ttatccgcct
      301 tcatggtgtt atcgctgtta ccggtgtccg gattaacacc ccggatatgc acaccggatt
      361 ccatcccgcc atcgcgggag cgccgggaaa ggaccaccac attgcggcgc agttttcccc
      421 tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc gcacgggttg
      481 cctcacgcag cacccggtta ttttctgcac cactcagaag ctgcaaatcg cggctgatgt
      541 cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcacctccca gccgacagag
      601 aatttccaga cgtccgccgg tcgcatccgg cacgggcagc ccgacaacgt tcaggatccg
      661 gtcacgccag ggaccactca gcacatgaag tcgtgacgct gccgtgattt cccgaccgga
      721 ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg actgcatccg
      781 ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca cccattcttc
      841 cgtcatttct ccggcagcat tacgggttaa caccgggttc agaacactta tcatctgtgt
      901 cagacgacct gcagatattg ccattccccc tcctcataac accgtcggac aacgcaaatc
      961 gtaaatcagc acggaaacag aaaacggcag ctccccctga atcagttctt cccgctccgc
     1021 aagatccgga ttccggtaca gcatcccggt cagtcgcatg gcagccccct tcatccgggt
     1081 taatgcctcg cccgggatca gttcaccgtc ctcacgaatc accttatccc ggctgccctg
     1141 aatgtaggcc agcagcacgg cggtagcctg acgaaccttg tccatcagca tgtcatcatc
     1201 cgcgtcatgg tcgacacgca gatgtgcctt gatctcttcc agtgtcagta atgccgtcat
     1261 tttcagcctc ctgcatcccg cccacgtttt gcagccaggg tccaggctga tgaatgagct
     1321 tctccgggtt tatcttcggt catactgttg cagtgccaca gcgagccccc ccacgtcacc
//
LOCUS       DAZGXS010000352         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGXS010000352 DAZGXS010000000
VERSION     DAZGXS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..268
                     /locus_tag="ACHKG5_005093"
     CDS             <1..268
                     /locus_tag="ACHKG5_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0870990.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..1170
                     /locus_tag="ACHKG5_005094"
     CDS             265..1170
                     /locus_tag="ACHKG5_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP0870991.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            1167..>1372
                     /locus_tag="ACHKG5_005095"
     CDS             1167..>1372
                     /locus_tag="ACHKG5_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP0870992.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          328 a          332 c          417 g          295 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcgtttgg tgatgtgata
      841 cgtcagggcg aaacaggcca ggttaaccag ctactggata ttctcagaca taaagcatta
      901 acgcagatgg cacaggagag tggcggtagt gcaaccgtgc gtctgaacac tctggactgg
      961 cttggtggtc agggccggga gcaggcagat aacgagtggc atgacgcgat taactggctg
     1021 ggagactggt gcagtgaaga gcagcatccg gtgatctggt caaccacgca ggctgcagaa
     1081 cacttaccgg ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga aagtatggtc
     1141 gatgaaatat ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg ctggtgctgt
     1201 ccggtggcgg ggcggtgggc gcttatcagg cgggagtggt taaggcactg gcagagtgtg
     1261 gtacacagat cagcatggtt tcaggggcca gcattggcgc attcaatggt gccattatcg
     1321 cggcctctac cgatctgtca gaagctgccg tacgcctgga ggcgctctgg ga
//
LOCUS       DAZGXS010000353         1371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGXS010000353 DAZGXS010000000
VERSION     DAZGXS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..371)
                     /locus_tag="ACHKG5_005096"
     CDS             complement(<1..371)
                     /locus_tag="ACHKG5_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0870993.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIR"
     gene            complement(346..891)
                     /locus_tag="ACHKG5_005097"
     CDS             complement(346..891)
                     /locus_tag="ACHKG5_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0870994.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            1278..>1371
                     /locus_tag="ACHKG5_005098"
     CDS             1278..>1371
                     /locus_tag="ACHKG5_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFP0870995.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDL"
BASE COUNT          373 a          367 c          294 g          337 t
ORIGIN      
        1 cggatggtag gctcgacgtg ggttttcata aagttctcgg catcaccatc cgtcggcaac
       61 cagataaggg tgttacgctg cttatgctct atgaagtagg cataaacacc cagcagcatt
      121 tttgaataac caacacgggc agacttcacc acattcacct cgcggatgta gtcgctgccc
      181 atcgcattca tgatagcccg ctgaaagggt agtgtttccc agcgcccttc ctggtatgcg
      241 gattctttcg ggagatagta actggcatcc gcccattcaa cggcagtctg tggctccggc
      301 ctgaacaggg ctcgcagccc ggcgcgtaca tcacgccgca gaatatcaat ctgactgttc
      361 gatatattca ctcagcaacc ccggtatcag ttcatccagc gcggctgctt tgttcatggc
      421 tttgataata tcccgtttca ggaaatcaac atgtcggttt tccagttccg gaaaacgccg
      481 ctgtactgag agagggatcc cgtcaagaat actggcaatt tcacctgcga tccgtgacag
      541 cacgaaagta cagaatgcgg tttccaccac ttctgcggag tctctggcat tcttcagctc
      601 ctgggcgtca gcctgcgcac gcgtaagtcg atggcgttcg tactcaatag tcccaggctg
      661 gagatctgcc tcgctggcag ccctgtaatc ctcaacctct ttacggagtt tttcattttc
      721 gatatcagct tccctctgcg cataccactg aattgccgtg gcggtatcaa atacagattc
      781 aacgccctta ccacctccgg agacgcaagg gagcccctga gactgccagc gttcaatcgt
      841 tcgcggatcc acgttgaaaa tttcggcaag tttcttttta ttaaccttca tgaaacagtc
      901 tcacaacaaa tacagggtcc gacatgaaag tgcccgaaaa tgactttttc aggcgttttc
      961 atgtcggacc tttacggatt cgatattaga aaaaacaaat agttatgttc gagaagtacc
     1021 gacatgattt tccccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg
     1081 taaccggtcg gatcgccgga aaggacccgc aaaatgataa taattatcat ctacatgtca
     1141 caacgtgcat ctacgccatc aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat
     1201 taattgatct gcatcaaatt aacgtaaaag caacttcaga taatacaaat cagcaacact
     1261 gaatatgggg caacattatg tcatcaaaga acagaacccg cagaacaaca acccgcaaca
     1321 tccgatttcc aaaccagatg attgaacaaa ttaacatcgc tcttgatctg a
//
LOCUS       DAZGXS010000354         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGXS010000354 DAZGXS010000000
VERSION     DAZGXS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            90..1318
                     /locus_tag="ACHKG5_005099"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ACHKG5_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          357 a          320 c          395 g          293 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataa
//
LOCUS       DAZGXS010000355         1361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGXS010000355 DAZGXS010000000
VERSION     DAZGXS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1094)
                     /locus_tag="ACHKG5_005100"
     CDS             complement(<1..1094)
                     /locus_tag="ACHKG5_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0870996.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAAR"
     gene            complement(1146..1355)
                     /locus_tag="ACHKG5_005101"
     CDS             complement(1146..1355)
                     /locus_tag="ACHKG5_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0870997.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
BASE COUNT          284 a          469 c          315 g          293 t
ORIGIN      
        1 cgggctgccg ccacggcctg ctgagcacgg tacacggctg cccttgcccg cgccgtggca
       61 atctgcgttc ccctgaactg tgcttccgcc agtgcaactt cattacgtgc agccgtcaca
      121 agtcctgccg tggcagacat cgctccggag gccatattgc caaagtaccg ggcaaccccg
      181 acggcaacca gcgcgcccac ggctgttgcc acattatcaa tctgtccggc aacaccgttc
      241 agcatgccgg agagcgtttt tgtcaccccg ctggcctcat tcgcaccgcc cacccaggcc
      301 ataaaggcgt tttccacctt tgtgatacta ctggaaaccg tttccggcat ggccgcatat
      361 tcatcacgta atatccccag ctggctgatt aacgcgggga ccactttatc cgctgtcagt
      421 tttccgtcat ccgccattgc cttcagatct ttacgggcca cgcccatacc cgcagccagt
      481 gcacgtacga tccggtcacc actttcattg accgaattaa actcctcacc acgcaataca
      541 ccctgcgcca gcgcctgact gaactgggtg atcaccgagc ccgcctctgc cgtactggca
      601 ccggagattt tcagccctgt cgaaatggcc tccgtcacct tcagcacatc atcagcactg
      661 taaccatatt cacgcatcga ggcagccgaa cgggcaaaca gggccgcatt atccgaaaat
      721 gcggtgcctg tccgctggct gatatccatc agcactttct gtgatgacgc aaattcatcc
      781 gatgactgcg acgcctgttt cagacgggca ttcacggagc tccattcatc cgccagcgaa
      841 atcaggtgtc cggtggcaaa ggcaccggca aacgcaccgg tcattccgac agccgaagcg
      901 cggatttccg tcaactggct gttcagctca gccagagccc ggcgctgctc cctggctgcc
      961 gcagcagcct gacgtccgcc attctgcagg gtccggtaat attcactgcc catacgggac
     1021 gcccgctgga tctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
     1081 cgtaacgtcg ccattcacct ttctccgggc aaaaaaaacc tgccacagca ggttttcatc
     1141 attatttatg acattgctgc aaggctcagc gcgtcttcca gcgccgcaaa cggatccacc
     1201 tccggcttat cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc
     1261 ccctgtgccc cgaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca
     1321 cccagcgggg ataccctgtc gaactccatc cacatcatcg c
//
LOCUS       DAZGXS010000356         1361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGXS010000356 DAZGXS010000000
VERSION     DAZGXS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            191..>1361
                     /locus_tag="ACHKG5_005102"
     CDS             191..>1361
                     /locus_tag="ACHKG5_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP0870998.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPALFLLMRMGIYKNIVLFFDKLKNSQEMTASKALDILAAKSPEGI"
BASE COUNT          454 a          242 c          250 g          415 t
ORIGIN      
        1 ggataccatt acaaggagct gttcccctcg ttgacgtcgc ggtttaccca cctcattacc
       61 aggttgatga cctgatgggg aaatgacgta aaaagacaaa taactctata aatgagcgaa
      121 atttgatagt tgatttttaa atagtaacct tattattact acaactttca tatggatgag
      181 gggaaagaaa atggttgata acgtaactgt tagcagagta tgtatacaat ctccttcttt
      241 cgtgcctgat ttggatggag aaaaaaataa atctcaatta ttcgttgacg atatagttgc
      301 gtatcttaaa agtccttcag tttattcact tgaaaaagag gggccgttaa atcattttgt
      361 taatcattgt tctgaagttg agttaggttt ctatagcgat ggtgcatatt caattcttgt
      421 ctccagatct aagcaacaac ctgaaggtat gattttaacc gtaagcgatg cagacgcaat
      481 caatatagta catatttccg tatctccagt gcttataaaa ttcctggatg atatttttac
      541 ttgccttcat acgtaccctg atgatgagag ttttacaaaa gagcagataa aagctaatag
      601 caaatatgat attgtagatt ataattgcct gttgcatttt actggaaaac caaaaagttt
      661 aatagaatgt agacattttg ctctgcaata ctgtatagat tcaatgaatg agcatacagg
      721 gaaagttcca ttaaaggctt actattcatc tccggaagat atacaaaaac atattccttt
      781 cgagcttgag cagcaattta acaatctaca aaaaaatcca ccacccggta catgcgtcgt
      841 tgccagtgat aagtttgggg aggcattatc tgtctttttt cacagaatgg aaaaagagaa
      901 gttaacgcat atgacggcaa tcgttcaatc tcaaacacac gctatggccg tccgcttgag
      961 gatcaaaaaa acgcctgctg gtgaaacaga atatgttgta tccttttatg atcctaatgc
     1021 aaccaatact gcagtacgct ataaagcaaa caactgtgat tcttttgggt cattgcaatc
     1081 gtttataaat attcaacagg caaaacaaaa atgggtaata acagatattt gctccgagtg
     1141 tgtaggaata accccttatc tccctcggga acaagcccat ttattaagtg gtattgaaaa
     1201 tgagttgcaa cctccattat caccaccagc attatttcta ttaatgagaa tggggatata
     1261 taaaaacatt gttctttttt tcgataaatt aaaaaactct caagaaatga cagcatcaaa
     1321 ggctcttgat attcttgctg cgaaatcacc tgaaggaata t
//
LOCUS       DAZGXS010000357         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGXS010000357 DAZGXS010000000
VERSION     DAZGXS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..1319
                     /gene="nleA"
                     /locus_tag="ACHKG5_005103"
     CDS             <1..1319
                     /gene="nleA"
                     /locus_tag="ACHKG5_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP0870999.1"
                     /translation="QPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVVNIP
                     DDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNVTQM
                     ALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPLKNA
                     LSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVKTEN
                     GWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNP
                     NTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPGLKP
                     DTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGADIRA
                     MEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          436 a          282 c          280 g          357 t
ORIGIN      
        1 ttcaaccgac catacaatct ggaatcacct cacaaaacaa tcaacatcat caaacagaac
       61 aaataccctc tacacaaata ccgcaatccg aattacctct aggatgccaa gctggatttg
      121 ttgttaatat tccagatgat atacagcaac atgcaccgga atgcggtgaa acaacagctc
      181 tactgagctt gataaaagat aaaggtctgc tctcagggct agacgaatat atagctcctc
      241 accttgaaga aggatccata ggaaaaaaaa cattggatat gtttggttta ttcaatgtta
      301 cccaaatggc attagagata cctagttccg tttcaggcat ctctggtaaa tatggtgtcc
      361 agctaaacat tgtaaaacca gatattcatc ctacatcagg taattatttt ttacagatat
      421 tccctctgca tgatgaaata ggttttaatt ttaaagacct tcctggcccg ttaaaaaatg
      481 cattaagcaa cagtaatata tcaaccactg cagtgtcgac tattgcatcg actggaacat
      541 cagccactac ttcgacggta accaccgagc caaaagaccc aataccatgg tttggattaa
      601 cagctcaagt ggttcgtaat catggtgtag aacttcctat agtcaaaact gaaaatggat
      661 ggaagcttgt tggagaaaca ccacttactc ctgatgggcc gaaagcaaat tacacggagg
      721 agtgggttat cagaccggga gaagcagatt ttaaatatgg tgcatctcca ttacaggcaa
      781 ctctagggct ggagtttggc gcacatttca agtgggattt agataaccct aatactaaat
      841 atgccgttct taccaatgct gccgcaaatg cgcttggtgc tttaggggga tttgcagtat
      901 ccagatttgc tagtacagat ccaatgttaa gtcctcatat cggtgcaatg gttgggcaag
      961 cagcagggca tgccatacag tataataccc ctggattaaa gccagacact attttatggt
     1021 gggctggtgc gacactgggg gctgccgatt taaacaaggc cgagtttgaa gtagctagat
     1081 tcactgacta tcctcgtata tggtggcacg caagagaagg agctattttc cccaataaag
     1141 cagatattga acatgccaca ggtgctgata tacgcgcaat ggaagaaggt atccctgttg
     1201 gacagcggca tccaaatcca gaggatgtgg taatcgatat cgaaagcaat ggcttaccac
     1261 atcataatcc atcaaatcat gttgatatct ttgatataat ccaagaaaca agagtctaag
     1321 ctttaacttg tttattttaa aataatagct gaaat
//
LOCUS       DAZGXS010000358         1351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGXS010000358 DAZGXS010000000
VERSION     DAZGXS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            42..1187
                     /gene="espG"
                     /locus_tag="ACHKG5_005104"
     CDS             42..1187
                     /gene="espG"
                     /locus_tag="ACHKG5_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP0871000.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT          454 a          278 c          246 g          373 t
ORIGIN      
        1 ttgccaaatt gttcattaca aaccagatag gagaatctct catgataaat ggacttaata
       61 atgactccgc atctttagtt ttagatgctg caatgaaagt taattctggg tttaaaaaaa
      121 gctgggatga gatgtcatgc gctgaaaagt tatttaaagt acttagtttt ggtttatgga
      181 atccaacgta cagtcgtagt gaaagacaat catttcaaga gttgttaacc gttttagagc
      241 ctgtatatcc acttcccaat gaattaggca gagtatctgc tcgtttttca gatggttcat
      301 ccttaagaat ttccgtcact aacagcgaac ttgttgaagc cgagattcgc acagcaaata
      361 atgaaaagat tactgtgctc ctggagtcaa acgaacaaaa taggttatta caatctttac
      421 ccatcgatcg ccacatgcca tacattcagg ttcatcgtgc cttatctgag atggacctga
      481 ctgatactac ctcaatgcgc aatctacttg gttttacgtc aaaactatca acaaccttga
      541 ttcctcataa tgctcaaaca gatccgcttt ccgggcctac accattcagc tctatcttta
      601 tggatacatg tcgaggactc ggcaatgcaa agctttcact caatggtgtt gatatacctg
      661 caaatgcaca aaaattgctt cgcgatgcac taggacttaa agacacacat tcatcaccaa
      721 cccggaatgt tatagatcat ggtatttctc gccatgatgc agagcaaata gcaagagaaa
      781 gcagcggcag tgataaacag aaagctgaag ttgtggaatt tttatgccat ccagaagcag
      841 caacggccat atgctcggct ttctatcaat ctttcaatgt gccagcctta acgttgacac
      901 atgaaaggat ctctaaagcc agtgaataca atgcggaaag atcattagat acacctaacg
      961 cttgcattaa catcagtatc tctcaatcat cagatggaaa catttatgtt accagccata
     1021 ctggggttct gataatggcg ccagaagacc gccccaatga gatgggcatg ttgacgaaca
     1081 ggacttctta tgaagtgccg caaggtgtga aatgtataat cgatgaaatg gtaagtgcgc
     1141 tacaaccaag gtatgccgca tctgaaacgt acttacaaaa cacttaaaat aaaactttat
     1201 taatcaaaac taataataga aaccacaccc ataattatta acaatttatt ccgaagttaa
     1261 tcacaattaa acaaagatat ttattctgca aacaatttac agcaaataga gaaaatgaaa
     1321 agaaaaccat taattgcatt tttcatcgcg a
//
LOCUS       DAZGXS010000359         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGXS010000359 DAZGXS010000000
VERSION     DAZGXS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..1143)
                     /gene="escV"
                     /locus_tag="ACHKG5_005105"
     CDS             complement(<1..1143)
                     /gene="escV"
                     /locus_tag="ACHKG5_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP0871001.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEES"
     gene            complement(1140..>1324)
                     /gene="cesL"
                     /locus_tag="ACHKG5_005106"
     CDS             complement(1140..>1324)
                     /gene="cesL"
                     /locus_tag="ACHKG5_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFP0871002.1"
                     /translation="FFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNT
                     FQQHVQIIERVRRMTS"
BASE COUNT          461 a          294 c          243 g          326 t
ORIGIN      
        1 actttcctca aagaaattcc aacgcatgac ctcaatttgc tgtgtaatgt ctgaactata
       61 aatattacta cttaagttta aaatcaatgg ctcagcgccc ggtgagatgt tttggctatt
      121 agacaaatca gctcccatag catccttatt attgccagtt ccaaatgttc ttttcttctg
      181 taatttccag gctatcccca acaggcaaac cgccagaaat aagaagacca atgcaggaaa
      241 gcccggaata atcgccatta cgagcattag tgcggcactg attaaaaaag ggcgactatt
      301 aacagaaacc tgttgaacaa tatcacctgc aaggttttct tctttttcgc tttcaccggg
      361 cacacgagta acaaccacgc ctgcggtgac agaaataata agcgcaggga tctgggcaac
      421 caaagcatca ccgacagaca atacagaaaa caggctaagt gccgcactaa atggcatgtc
      481 aaattgccac ataccaatga gcacgccacc aaaaaggtta actaaaacaa taatgatacc
      541 agcaatagcg tcccctttta caaacttcat cgccccgtcc attgaaccat aaaactggct
      601 ttctttttgc actcgggagc gaagaacttt tgcctcaagc gggtcaataa cgcctgcgcg
      661 catatcacca tcaatactca tttgctttcc tggcatacca tcaagggaaa aacgagcact
      721 cacttcagca acacgctcgg ccccttttgt aataaccata aattgaacaa tggtgataat
      781 agtaaaaata accaggccaa caacaatatt accgccaaca acaaagttac cgaaggagta
      841 aataatgtca cccgcatcgt gatgcagcag aataagtctg gttgtactaa cactgagcga
      901 caggcgcatc aacgtcgtaa tcaacaggat tgtggggaaa gaagttagtt caagaggatt
      961 ttttatatag attgagagca ttaataaaag taaagctgtc gaaatattaa tcgcgataat
     1021 gatatccacc acaactgttg gtagtggaat aatcatcatc attacagcca taaagaagaa
     1081 gagagccaga ataagatcgt gatatgactc tgctttttta aatatattta agagtttatt
     1141 catgatgtca tcctgcgaac gcgctcaata atctgcacat gctgttgaaa agtattcatt
     1201 agaaaaaaaa catcgatatt ggttaacaaa caacttaagc acaatttatt atcgtagaca
     1261 aaaatatgtt caatcactcc ctgcgtacgc tcaggagcaa gggcactaaa gaaagagaaa
     1321 aatg
//
LOCUS       DAZGXS010000360         1321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGXS010000360 DAZGXS010000000
VERSION     DAZGXS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            148..1107
                     /locus_tag="ACHKG5_005107"
     CDS             148..1107
                     /locus_tag="ACHKG5_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP0871003.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(1251..1321)
                     /locus_tag="ACHKG5_005108"
                     /pseudo
     CDS             complement(1251..1321)
                     /locus_tag="ACHKG5_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          468 a          249 c          245 g          359 t
ORIGIN      
        1 ttaaaagata ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata
       61 aaacatcata ttgattacgc accttgcatt acaaactcat gtatgtaaaa tacgcgcacc
      121 attcaaaaaa aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt
      181 gctctgtcat ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat
      241 gatgatgcag ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac
      301 agagatgatt actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc
      361 caattatctc tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac
      421 aacacagctg taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg
      481 aactcccttg cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc
      541 cccgacgcag gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa
      601 tatcatgcat gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta
      661 tccatgacac caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct
      721 gctgttactg gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat
      781 gataaaacct tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat
      841 atgtccgata caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc
      901 aatcgcaact atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta
      961 aacaaaactg ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc
     1021 acactagcat ttaataaagt aacatcagaa ttcaagacag gggatgacta ttcatttata
     1081 aatggagcaa tcaccttctt tttttaactg aattgaattc aatcaaaata acataagtcc
     1141 aacaaaaaca taaagtgcga aatgaatgcc agctccattt atttcgcact ataaaagatt
     1201 aaaagttgca ataaaataat aaaatgactc agttacgaaa accaataaac tgtggccagt
     1261 agtgagtcgc tcatcatcgg gctttttggc gaatgaaatt tagctacgct ttcgagtctc
     1321 a
//
LOCUS       DAZGXS010000361         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGXS010000361 DAZGXS010000000
VERSION     DAZGXS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1319
                     /locus_tag="ACHKG5_005109"
     CDS             <1..>1319
                     /locus_tag="ACHKG5_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0871004.1"
                     /translation="QYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSRLVTTAITADTEH
                     RSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLA
                     VYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNL
                     VGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSIT
                     ETRNEITQTVSKTLEDQSAIIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGA
                     GIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITS
                     SANPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLV
                     KTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAK"
BASE COUNT          323 a          333 c          398 g          265 t
ORIGIN      
        1 cagtatctgg cgcaggtgaa atgggacacg ccgcgggtgg tgaagggcgt gcgcttcagt
       61 ctgcgcctga ccagcggaag cggagaaggc agccgtctgg tgaccaccgc catcactgcg
      121 gatacagagc atcgttccag tggtctgccg ccgggggaat acaccctgac ggtcagggcg
      181 attaacagtt atggccagca gggggaaccg gccaccacca cgttcaggat taatgcacct
      241 gcggtacccg ccacgattga gctgacaccg ggctattttc agataacagc ggtcccgcgt
      301 cttgcggtgt atgacccgac ggtacagttt gagttctggt tttcggagac aaaaatcgca
      361 gatacatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca gtggagtgta
      421 tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag cgtcaacctg
      481 gtggggaaat ctgcttttgt ggaagccagt ggccgggcca gcaatgatgc agaagggtat
      541 ctggggctgt ttcgggaaaa aataggaaaa ctgcatctgg ctcaggggct gtgggagctg
      601 atagacaaca gccagcttgc ggatgagatg gcggagatga agacctccat caccgaaacc
      661 cgcaatgaaa tcacacagac ggtcagtaaa acgctggagg accagagcgc catcatacag
      721 cagatacagc gcgtgcagaa ggacacaaat gatgaccttg ctgcacttta catgctgaag
      781 gtacagaaaa caaaaaatgg cataccctat gttgccggta ttggagcggg gattgaggat
      841 actgatggcc agcccctgag caacatactg ctgctggctg accgtattgc gatgattaac
      901 ccggaggacg gcaacaccac gccgttattt gtggcgcagg ggaatcagtt gttcatgaac
      961 gatgtgttcc tgaaacgact gtttgcggtg agtatcacgt catccgccaa tcccccgacg
     1021 ttttccctga cgccggaggg caggctgacc gcaagaaatg ctgatatcag cggtaacgtg
     1081 aatgcgaatt ccgggacgct caacaacgtc acgattaacg agaactgtcg ggttctggga
     1141 aaactgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa agctttcccc
     1201 cgggattccc gtgcaccgga gcggtggcca tcagggacca ttaccgtcag ggtttatgac
     1261 gatcagccgt ttgaccggca gattgttatt ccggcggtgg cattcagcgg cgctaaaca
//
LOCUS       DAZGXS010000362         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGXS010000362 DAZGXS010000000
VERSION     DAZGXS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(39..227)
                     /locus_tag="ACHKG5_005110"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHKG5_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          385 a          290 c          237 g          400 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg acctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat tt
//
LOCUS       DAZGXS010000363         1303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGXS010000363 DAZGXS010000000
VERSION     DAZGXS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1303
                     /locus_tag="ACHKG5_005111"
     CDS             <1..>1303
                     /locus_tag="ACHKG5_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0871005.1"
                     /translation="ADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRA
                     EEARKQHTASLSSPDTPRPLSDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLP
                     AAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDAG
                     RVVEQLNPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHS
                     GYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPD
                     GLERRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQL
                     LAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYN
                     AAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT          280 a          384 c          451 g          188 t
ORIGIN      
        1 tggcggaccg cttcgggcgg acgctgacgt acaggcgtga ggccgccggt gacctggccg
       61 gggaaatcac cggcgtgacg gacggtgccg ggcgggagtt ccgtctggtg ctgaccacgc
      121 aggcgcagcg ggcggaagag gcccgtaaac agcacaccgc ttctttatct tcccctgaca
      181 ccccccgccc tctttcagac tcagcgttcc ccgacacact gcccggtacc gaatacggtc
      241 ccgacagagg tatccgcctt tcggcggtgt ggctgacgca cgacccggca tacccggaga
      301 gcctgcccgc tgcgccactg gtgcggtaca cgtatacgga agccggtgaa ctgctggcgg
      361 tatatgaccg cagcaatacg caggtgcgcg ctttcacgta tgacgcgcag cacccgggcc
      421 ggatggtggg gcaccgttat gcgggaaggc cggagatgcg ctaccgctac gacgatgcgg
      481 ggcgggtggt ggagcaactg aacccggcag gcctgagtta ccactaccag tatgagcagg
      541 accgcatcac cgtcacggac agcctgaacc ggcgtgaggt gctgcataca gaaggcgggg
      601 ccgggctgaa gcgggtggtg aaaaaagaac tggcggacgg cagcgtcacg cacagcgggt
      661 atgacgcggc aggaaggcta acggcgcaga cggacgcggc gggacggcgg acagagtatg
      721 gtctgaatgt ggtatccggc gatatcacgg acatcaccac accggacggg cgggagacga
      781 aattttacta taacgacggg aaccagctga cggcggtggt gtccccggac gggctggaga
      841 gacgccgggc atatgatgaa ccgggcaggc tggtatcgga gacatcgcgc agcggggaga
      901 cagtacgcta ccgctacgat gatgcgtaca gtgagttacc ggcgacgaca acagatgcga
      961 cgggcagcac ccggcagatg acctggagcc gctacggtca gttgctggcg ttcaccgact
     1021 gctcgggcta ccagacccgc tatgaatacg accgcttcgg ccagatgacg gcggtccacc
     1081 gcgaggaagg catcagcctt taccgccgct atgacaaccg tggccggtta atctcggtga
     1141 aagacgcaca gggccatgaa acgcggtatg agtacaacgc cgcaggcgac ctgactgccg
     1201 ttatcacccc ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca
     1261 gcaccacgca gggcgggctg acgcgcagta tggaatacga cct
//
LOCUS       DAZGXS010000364         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGXS010000364 DAZGXS010000000
VERSION     DAZGXS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(115..>1296)
                     /locus_tag="ACHKG5_005112"
     CDS             complement(115..>1296)
                     /locus_tag="ACHKG5_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0871006.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
BASE COUNT          303 a          404 c          258 g          331 t
ORIGIN      
        1 accacgaaag atattaactt cttgtctagc tggctctatg tccactggag aacaaccata
       61 caataaaaat aaaaaataaa actaaaaata tatatttttt catatttcct cctgttaatt
      121 gacccagcat tcttgccacg ttctacaagc cagagtcata actgaagcgt caacacctgc
      181 accgatacca acagattctg tcgatgattc tggattatca acatccacta cataagtata
      241 cgcaaaatgc cccccaactc cgccgccaac cgttacacct actgaattgc tgactcccga
      301 tgtaggtaaa gacttggtct cagaatatgt gatagccgca tttatcccac caccaattcc
      361 tgcatgtccg catgctgtag cataaataca aatatcagat aacatttcac ctttcttttt
      421 agcaaacgca acgccaagtc ctgcatgacc acctaatgat attagatctc cactgccagt
      481 aatatcaatt gatgatgttc catatgaatc atcatgccaa acatcatata ataattgata
      541 taaattatac aaacccaaag gatccatcct tattatcgga ttcagtgggt aattatacag
      601 attccatccc cccgccagcc caatggggtc ttgtgtaata tacctcccct gtagcgggtc
      661 atagtaccgg ttccggttat agtacagccc tgattcctca tcatgctgct gccccggcag
      721 acggtatggc tggtgcaggt aatacgggtt ctcttcatta agctggttgc cccattcatc
      781 atactccccg cgccacgccg tattgccgtc ttcgctgatg agcgccagcg gcaggccccg
      841 gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg tattccggct ccacctgtct
      901 ggccagttgc tccaccgtca gcccgcactg cgcaagccac gcccggcttt cactgctcac
      961 gcggtctgcc cggatttctt cctccagcct gtccagcagc cgcaccagtt cagccgggaa
     1021 caccacgccg tggccgttct cactcccttc ctgctggagc gtctctgcca ggctgcgccg
     1081 ctgcgctttt tcccgctcgc cgttctctgt ctcgacccgg atgagcggcg tgaagcttcc
     1141 cggctcgtat accgtctgga tacgtgtggt gtcagtctgc accgtcgtca gcctgtctcc
     1201 gtcccagcca taccacgtca cctccggttt acgcgacagc gacatccacc ccgtcaggtc
     1261 acgctcccgc cgccagaccc gttttgccat tcgccg
//
LOCUS       DAZGXS010000365         1294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGXS010000365 DAZGXS010000000
VERSION     DAZGXS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1294
                     /gene="rhsD"
                     /locus_tag="ACHKG5_005113"
     CDS             <1..>1294
                     /gene="rhsD"
                     /locus_tag="ACHKG5_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFP0871007.1"
                     /translation="ADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRA
                     EEARTSSLSSSDSSRPLSASPFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAP
                     LARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVV
                     EQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYD
                     AAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLE
                     SRRAYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAF
                     TDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAG
                     DLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT          275 a          380 c          442 g          197 t
ORIGIN      
        1 tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc gacctggccg
       61 gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta ctgaccacac
      121 aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac agttcccgcc
      181 ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc cccgacaggg
      241 gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa agcctgcccg
      301 gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg gtatatgacc
      361 gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcatccgggc cggatggtgg
      421 cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc gggcgggtgg
      481 tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag gaccgcatca
      541 ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga
      601 agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg
      661 caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac ggtctgaatg
      721 tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg aaattttact
      781 ataacgacgg gaaccagctg acggcggtgg tgtccccgga cggactggag agccgccggg
      841 catatgatga accgggcagg ctggtatcgg agacatcgcg ctgtggggac gtcatccggt
      901 atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg acgggcagca
      961 cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac tgctcgggct
     1021 accagacccg ttatgaatac gaccgctttg gtcagatgac ggcggtccac cgtgaggaag
     1081 gtatcagccg ttaccgccgc tatgacaacc gtggccggtt aacctcggtg aaagacgcac
     1141 agggccatga aacgcggtat gaatacaacg ccgcaggcga cctgactgcc gttatcactc
     1201 cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaagcggtc agcaccacgc
     1261 agggcgggct gacgcgcagt atggagtatg acct
//
LOCUS       DAZGXS010000366         1288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGXS010000366 DAZGXS010000000
VERSION     DAZGXS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>1288)
                     /locus_tag="ACHKG5_005114"
     CDS             complement(<1..>1288)
                     /locus_tag="ACHKG5_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP0871008.1"
                     /translation="HPGSSLDKAINNTRVKNVSTDVKHGQIQERKRNFIYKKNDDISS
                     RFKLYSSLVKQKNATEDVVLIGKMILDEVRSYRTIHNDRNIVSNSGNWKTSFLCNLAR
                     LLYSIFNGSNYFCSREGENNSSPSSTLLTIHQPEKQELLQQKSIKHLPTSNNIDGYIK
                     IRKTRGAEDQTTTITQSLIINELLNGVDRNTIPFQKISELNDIIHSYENMQIKNSRKG
                     IEILVKQGELLSSLINDNKGNKQLSDNASKIINLLGIEYQSHKVDIEPFIHAVWVAGA
                     PPDNTFSYITAFLNTYKDYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNPHL
                     AEEMNEVILKIQNIQNETIEFKETRERLKELENRYKSLTSETKEKFNVFFLESMIGMQ
                     DNYFTYCISNGISNTDDISRLDFLTNVLKLSPEVQND"
BASE COUNT          373 a          207 c          183 g          525 t
ORIGIN      
        1 gtcattttga acttctggtg aaagttttaa aacgttagtt aaaaagtcaa gtctggatat
       61 atcatcagta tttgatattc cgtttgatat acaatatgta aaatagttgt cttgcatacc
      121 tatcattgac tcaagaaaaa aaacattgaa tttttcttta gtttcactcg ttaatgattt
      181 atatctattt tcaagttcct tcaatctttc tcttgtttcc ttaaattcta tagtttcatt
      241 ttgtatgttt tgtattttta atatcacttc attcatttct tccgctaaat gtggattcgt
      301 tcttctcaaa cgcataattg cataattcat cgctatattt tttaaaatcc cactaaactt
      361 ggccgcacca aaggcattag gatcaatcca gagcaggtag gtataatctt tatatgtatt
      421 taaaaatgcc gttatatatg aaaatgtatt atcaggaggt gcgccagcaa cccatactgc
      481 atgtataaat ggctctatgt ctactttatg tgactgatac tctataccca ataagtttat
      541 tattttagat gcattgtctg ataattgttt atttccttta ttatcattta ttaatgatga
      601 taacagctct ccctgcttaa caagtatttc tatacctttt cgactatttt taatttgcat
      661 attttcatat gaatgtatga tatcattgag ctcacttatt ttctgaaaag ggatggtatt
      721 tctatcaact ccatttaaca actcattaat tatcaaactt tgagtgatag ttgttgtttg
      781 atcttcagcg cctcttgttt ttcttatttt aatgtatccg tcgatgttat tacttgttgg
      841 taaatgtttg atactctttt gttgtaatag ttcctgcttt tcaggctgat gtatagtaag
      901 tagagtagaa ctgggggatg aattattttc accctctcgg gaacaaaaat agttactacc
      961 attaaatatg ctatatagta gtctagcaag attacataaa aaagatgttt tccagtttcc
     1021 tgagttactt acgatatttc gatcattatg tatagttctg taacttctaa cttcatctaa
     1081 aatcattttg cctattaaaa caacatcttc tgtcgcattt ttctgcttta cgagcgaact
     1141 gtaaagttta aatctggatg agatatcatc atttttttta tagataaagt tcctttttct
     1201 ctcttgaatt tggccatgtt taacatcagt actaacattt ttaactcttg tattgttaat
     1261 tgctttatct aaagaagagc caggatga
//
LOCUS       DAZGXS010000367         1259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGXS010000367 DAZGXS010000000
VERSION     DAZGXS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            128..730
                     /gene="ais"
                     /locus_tag="ACHKG5_005115"
     CDS             128..730
                     /gene="ais"
                     /locus_tag="ACHKG5_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0871009.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKG5_005116"
     CDS             complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKG5_005116"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0871010.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          346 a          278 c          273 g          362 t
ORIGIN      
        1 aaaattttga ggcattttgc cagcatcgta ggatttttaa tattacacca actgctaatt
       61 atcgccagaa atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg
      121 agtttacgtg ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt
      181 actggcgctc gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt
      241 gccacgtatc gacaataaaa cgctgggcag actggcacag cagcacccgg ttgtcgtttt
      301 gtttcgccat gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg
      361 tattacggtt aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat
      421 ccctgatttc gatctttatt ccagtaatac cgtccggacc attcagtccg cgacctggtt
      481 ttcagcgggt aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta
      541 tagtgcgatt aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca
      601 taatcattgc ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta
      661 tctggatggt ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt
      721 taatcactaa attttcaatc tgacagccag taatggctgt catcattgtt acagaagacc
      781 tttcaaacgt aacgtttttc gggtggcgac attcaaatca tagtgcgcca gatcttcagg
      841 ttttacccac gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac
      901 gcaatcaaaa atcaggtaaa tcatataaat ttcttccttg cgaccatctg catacgtctt
      961 ggtgcgaata tcatcgctga aggtccaggg cgtgatttct gtcaaaagca gctgttctcc
     1021 cagttcttcg cgaatttcgc ggcgtagagc ctcttcaatt cgttcgccgg gctccacgcc
     1081 gccacccgaa agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag
     1141 caaataagca ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt
     1201 attccttata aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtg
//
LOCUS       DAZGXS010000368         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGXS010000368 DAZGXS010000000
VERSION     DAZGXS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            213..320
                     /gene="yniD"
                     /locus_tag="ACHKG5_005117"
     CDS             213..320
                     /gene="yniD"
                     /locus_tag="ACHKG5_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0871011.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(373..1131)
                     /locus_tag="ACHKG5_005118"
     CDS             complement(373..1131)
                     /locus_tag="ACHKG5_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0871012.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          359 a          293 c          288 g          318 t
ORIGIN      
        1 tgagttttag tgctgatgaa ttaaagacgt atttataacc agctatttac gatatttata
       61 tatcgataca taattttatt ttttcataaa acgcagaaac agtgatcagg tgtacaacgc
      121 cacccgacgg ctgtaataac aaaaaatccc ttgatgcctg tcccttttgt tacactccgt
      181 tatcacgcac aagagatatg caggacactg gtatgccgac taaacgcttt gataaaaaac
      241 actggaagat ggtggtggtg ctactggcaa tctgtggcgc tatgttgttg ctacgttggg
      301 cagcaatgat ttggggctga gcagtgacaa acgaagacag ccagaccgca taacacggtc
      361 cggcagataa ttttacatgc tgtagcccag cgataaggtt gtccgacggt cggtatgttc
      421 cggtgcagac tctggaggtt cagagttcca tgtgacgttg taagccacct tcagtccaaa
      481 atgctcatta atagcaacat ttaatgcgct ttcggagttc agcgttgtgt cttccgcgcc
      541 aaagacggaa acaccctgcg taaatttagc gttgtcagta agctgccatg cataagcacc
      601 ggaggcatag cccagcggct gagtttcact ggcattgtcg gtatatttgt cgtaacgcac
      661 acctggaccg aattcaaagc ggaaactgtg taccgggcca ttcaaaaact gacgaccata
      721 acccgcggtc aacacatcgc gctctcgata gccgttataa cggtctgtca gccagcttgc
      781 ctgaccaaat aaatagtcat aatcagttaa attaaaacgg ctacgcccgc ccgccgcata
      841 tttttctgaa gaacgctcat cattagaaga agtattactg gcgttccccc acagcgacca
      901 ggcagtggtt tgtccatacc aggtcatggt ggtgtcagcc gtaagtgagg agcttttcgt
      961 attgcctgat tgtgcaagat accctgcgtt cagattacct tcgaaaggtt ttttagcgct
     1021 ggcaggatcg tccatgacag taaaaacgga atcatcggca gctgcattca gtgacgcaaa
     1081 catgcccccc gccaacataa cgatggcagg aactgtcttc aaaagcttca tttatcaaga
     1141 gtccgtacaa caaaaaaaga gaccatcgcg gtcccggaaa ctttcttaag gatcaaagat
     1201 tagcgtccct ggaaaggtaa cgaattataa aaaggcgcga ataacttatc aatgtatt
//
LOCUS       DAZGXS010000369         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGXS010000369 DAZGXS010000000
VERSION     DAZGXS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..139
                     /locus_tag="ACHKG5_005119"
     CDS             <1..139
                     /locus_tag="ACHKG5_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871013.1"
                     /translation="KKNKEMSEIVAGTILQLLRQTQDNQFSTLGKKNGHMKSIDNKSS
                     L"
     gene            212..781
                     /locus_tag="ACHKG5_005120"
     CDS             212..781
                     /locus_tag="ACHKG5_005120"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFP0871014.1"
                     /translation="MTSRDQGINSELSNRTIQIKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            741..>1255
                     /locus_tag="ACHKG5_005121"
     CDS             741..>1255
                     /locus_tag="ACHKG5_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFP0871015.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIG"
BASE COUNT          458 a          225 c          259 g          313 t
ORIGIN      
        1 caaaaaaaat aaagaaatga gtgagattgt cgctggaacc attctccagt tattacgtca
       61 gactcaggat aaccaatttt ctacgttggg gaaaaagaat gggcacatga agtctattga
      121 caacaaatcg tctctctagg ttgacaggaa tccatctctt tctttgcaaa caaaaggagc
      181 cgagcaattt tctacaaaga aacttctcaa tatgacttca cgagatcagg gtattaattc
      241 tgaactatct aatcgaacga tacaaattaa ggaaaaaatc cataatggta tccatacaga
      301 atacatcaca gatcaaaaac acagtaataa taaagatcgt gaaaaaaaat accgcgatgg
      361 agacaaaata aatggaccgc aggcccattc attagacatt actaacgaaa gaaggtttgc
      421 tgataacaga accatgttta ctcaacatat cgaaaagcaa aggaatgtta atacgctcaa
      481 tcagaatgat ataaataatt ctgctaataa tgccaatgtg cgcgaaaatg aattgacata
      541 tcagtttcag cgttggggac aaaatcatac ggtccggatt ctggaaagta gtgaagggat
      601 acgactaaaa ccatcagaca cacttgtctc cgacagactc catgaagccc aacacaatga
      661 tgttacagca caacgttggg tattgactga gcaagatgaa cgacaaggac aacgacatca
      721 gcctcatgag gagcaagaaa atgaaggcaa attcgaaaac gatcagaagg atgaaagtta
      781 atgttcggtc tgaggaaagt aaatcgaaac actctacgtt tgaaaccact tttcaaaatt
      841 ggaaagaaaa tggcgaagat gttgcattat taatgcccga atttagtgct aaatggctac
      901 cgatagcaga agagagtggc agctggtctg gatgggtgtt gcttcgagaa atatttccct
      961 taatttctgc cgagctcgct gggatggctt taatgccaga aacggaaagg ttaatagggg
     1021 aatggctcag tttatccagt tccccgttaa atctaaagta tcctgaacta aaatataatc
     1081 gtttgtgcgt aggtaaagta tttgatggag tattgagtcc agctcaacca ctgataagaa
     1141 tatggacagg ggaattgaat ctttggttag ataaagtcac agtctgccaa tacgaaaacg
     1201 ctccaacttt agacaaaaaa tcattatatt ggcccattca ttttgtaatt ggatt
//
LOCUS       DAZGXS010000370         1249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGXS010000370 DAZGXS010000000
VERSION     DAZGXS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            149..436
                     /locus_tag="ACHKG5_005122"
                     /pseudo
     CDS             149..436
                     /locus_tag="ACHKG5_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            880..>1249
                     /gene="espR1"
                     /locus_tag="ACHKG5_005123"
     CDS             880..>1249
                     /gene="espR1"
                     /locus_tag="ACHKG5_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFP0871016.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQG"
BASE COUNT          469 a          247 c          182 g          351 t
ORIGIN      
        1 ttaatctgga agcgcaaaat aataatctaa aaaaattaat atttctgata gttataaact
       61 gaaatttctt aatcttgatt ataacaagct agcatcgctg gatgtctccc ggcaagagtc
      121 tctgattgag ttaagtgccc atcacaatat gatcactgac cttatattac acaatcatcc
      181 caggatgaaa acaatcactt taaacgacaa ccatattgca catttaaaca ccaaaactac
      241 tacaaaactt gaatatttaa acttaagcaa taacaattta ttgccaacca atgacattga
      301 tcaactaata tcatcaaaac atctttggca tgtattagtt aacggcatca acaatgattc
      361 acttgcacaa atgcagtact ggactgcagt aagaaatata attgatgaca ctaatgaagt
      421 gaccattgat ttatcttata atctggcaat cacaaatatc gataccagtg atgaacatct
      481 tgtagaagta agcgataatt cagaaggtaa ttatataaaa gacaatgact caatgtctat
      541 tcgttataga tcaaaatatt attccagaga ttccgcctta atagaagaag aaacaatatt
      601 ttctgacgca gaactaaaag ctattctgcc tatgcgtcgc atgtacgggg ttagtgacta
      661 taagtcaaat tcctcttctc tatcctcaca ctcgggactc aaggacccaa cgggcacacc
      721 cgtctgttat tatattcata atgagagtaa accttcctta ggttatggtc caacatccaa
      781 taattggtta agccaatcct ttacaacaga gttataaaac acatcctggt ttatcaaggg
      841 tgataaatca gaaaaacatt gtattactat ggagcagtta tgaaattccc ttcaatattt
      901 aacaaaataa aaccacaatc catacagcaa catccagaaa aaaatcaact taactggatg
      961 ctcgaattaa ataaatggaa agaagaacgt atacttacag gtgaaatcca tcgtccggaa
     1021 tgtcgaaacg aagccgctaa aaggataaac tgtgcttttt tgtcgaaaca gaatgacatt
     1081 gatttatcag gacttaattt atctactcaa ccaccagggc tgcaaaactt cacctctatc
     1141 aatcttgata ataaccaact cacacatttt gatgcaacca actacgatag actcgtaaaa
     1201 cttagtctga atagtaacac tcttgagtca ataaatattc atcaaggca
//
LOCUS       DAZGXS010000371         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGXS010000371 DAZGXS010000000
VERSION     DAZGXS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            64..696
                     /locus_tag="ACHKG5_005124"
     CDS             64..696
                     /locus_tag="ACHKG5_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0871017.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(715..1218)
                     /locus_tag="ACHKG5_005125"
     CDS             complement(715..1218)
                     /locus_tag="ACHKG5_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0871018.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
BASE COUNT          399 a          197 c          238 g          407 t
ORIGIN      
        1 aataataatg aaagggatga ttataaatga atgcgttaaa gcatttacac tcaggatgaa
       61 tatatgggaa aaattaaaat tgtagtttca gatcagcagc cgtttatgat tgatgggata
      121 attggattcc tcggacatta tcccgattta tatgaggttg ttgggggcta taaagatctg
      181 aagaaagcta tagcggagtg taacaaatct gcagcccaaa tatttatttt aggagaattt
      241 gcaggtggaa tgatgggggc cgaactcgta aaatgggtta aatcgcataa aatagatgct
      301 catattatta catttgtagc aaaaatgccg tatattgatt caataaaatt gcttgaagca
      361 ggtgcgaaag gatgcgtatg gaaaaccagt cacccggcga aacttaatcg cgctattgat
      421 tcgattagta acggctacac ttattttgat agtgtacata tggattgtga gaaaatatct
      481 tccagatatt cctctgataa tcaacttaca aatcgtgagt ctgaaatatt acaactcata
      541 gctgatggaa aaacaaacaa agaaatcgct aatttcttgc agttaagcag gaaaacagtc
      601 gaaactcata gactcaatat catgaagaaa ttagatgttc acagtggtat cgagttgatc
      661 aaaacagctt tacgtatggg tgtgtgcact atataaaaaa aagaggcttt aggctcattt
      721 tgctaaagcc ttacgattgt ctagttcatt aagcctggta tatacaatat atattttttt
      781 ggcatattgc agtcgctttt tttggttatc cattgcaaaa cccgcattat aagtccccag
      841 gcattgccac gtcattccac aacgggaaaa atgcttatat aaaatctcgg ttcctatttt
      901 aatgttcagg cagggattat ctaacaaatc tcgttcatct tttattattc cacgtttttt
      961 taacatggga atgtgcatct ggtttatctg cattaaacca taatcaaaac tctttatatt
     1021 gtttttctta tctctgttct tccctatgct atcctttttt agattgcttt caacaagagc
     1081 gatagcttta atcaaatttg gttcgattct gaacattgtt cctgcttcat taaagcaagc
     1141 gctactggcg aaagacattt ttgtaattaa tatagaaagc gtgaagatgg ttaagaattt
     1201 aacttttggt gagcgcatat atctttatga gtcaggattg t
//
LOCUS       DAZGXS010000372         1239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGXS010000372 DAZGXS010000000
VERSION     DAZGXS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>1239)
                     /gene="espX6"
                     /locus_tag="ACHKG5_005126"
     CDS             complement(<1..>1239)
                     /gene="espX6"
                     /locus_tag="ACHKG5_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFP0871019.1"
                     /translation="CNVKTEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHNDPQQ
                     VDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDNNNK
                     TETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIR
                     SSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSERLTRKLSISD
                     AGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKMALRNVNFNG
                     SILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNFKNANLRNSN
                     FNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGTPKVILTGAI
                     LNYSDLSGKDLSEYNLTGIL"
BASE COUNT          338 a          217 c          244 g          440 t
ORIGIN      
        1 agagaatacc agtaagatta tattctgaaa gatcttttcc cgatagatcg gaataattta
       61 gtattgcgcc agtaagaatt actttaggtg ttcccggtat ttgaggtgtt ccaggtattc
      121 caggtatttt agatgtttca tatagaatca cggttgtgaa atctatgtgt tcaagatacg
      181 cgttactaag gtcacttcca gtaaaggtta cgccagtgag aacttttccg ttgaagttac
      241 tgtttcgtag atttgcgttt ttgaaattta cataagtcaa gtggcgaact ttacgaaaat
      301 cattattttc taatgaggca ttttcgaatt ctgcattacg cagatcacaa tcggtaaaat
      361 ccacgccttc acagatcgta ccggagaact tggcatttct aagaatgctg ccattaaaat
      421 tgacattcct tagcgccatt ttcgacaagt ctatgtcggt taaatccatc tctgctaaat
      481 tcaacccttc gatacaccgt tgacccgttg catcttcagg aatagcagtg ctaatagtat
      541 tgatgacaac atcacctatc ttgaaggtca ttgtaattcc tgcatcagag atagagagtt
      601 ttcgcgtaag acgttcactc ttatctccag tgatactctt ctgagcaacg taaaagcgct
      661 cgcatgtctc acaaaatctt ttccagctct catttggggt cagattattg tccgggagga
      721 aattagcaat gctataaaga tcgttcagga cgctacttct tatttcagga gaggcgcatt
      781 caataatttc agaggtggat attgtatttt taattttatc tatattatcc gtgagatttc
      841 gtgatcctgc tatatttggc tgctggtggt tttctttgta gtattgcagc aaattttccc
      901 attgtaagtt tgtaaacgag aaagtttcag tcttgttatt gttatcccgt atggttaatt
      961 gattttctac tgggtcaaaa gaaatcatga agttattcag gtgttgatat tgatgagtgg
     1021 catctaattt cacttcaaat gaatttgaac ttttgcaggc atgttttaaa aacttttcac
     1081 agggaaggtg ttgatcttta tctacttgtt gtgggtcatt gtgcaaaaca gagaaaatac
     1141 tctctttcaa attgttcttg tcaatataga ttggtagttg cgctttttgt tccattattt
     1201 taataaactg ctgcagttgc tcggttttaa cattgcagt
//
LOCUS       DAZGXS010000373         1224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGXS010000373 DAZGXS010000000
VERSION     DAZGXS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1224
                     /locus_tag="ACHKG5_005127"
     CDS             <1..>1224
                     /locus_tag="ACHKG5_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871020.1"
                     /translation="YRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGE
                     FVDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELS
                     DASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAY
                     KSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSE
                     IVDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTY
                     DESGISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFLIKCINSLTGLVGIPG
                     LPDKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYT
                     EPKTEDPRFRYVSSN"
BASE COUNT          400 a          192 c          254 g          378 t
ORIGIN      
        1 aatatagaga taaacgcatt gctaaatttg actttattaa atttgtatct gaaagtgatt
       61 ttcaactcag tgaacttgat cagttagcac aagttgaatc tcggcaaatt acttccgaaa
      121 ttagaggtgg ggagtttgtt gacgatgatt ttaggcaact tgtagagatg cttaagctga
      181 atatgatttg cacatgggat cttcggtcta gcgtgtatta cttaacaatg aaaatgcttg
      241 gtcagcccgg tactcctgaa tatgcgaaat atagtgaact aagtgcatcg atttttaatg
      301 aattatcaga tgcttctgat acgaaagggt actggtcaac cgatgtgaag ctagtgagtt
      361 catctggaca tgaatatact gagcgtgaat ttgaactaga aagggaaaaa tctactcgcg
      421 gtaaaggagg tatgactcgt tcacttgaaa tgtttatcgc atcgctaaat tggttggcat
      481 ataaatctat ttattacagc gtcattgcta agcattttaa agcagactca tttattcatc
      541 caataaggca tgcatatcaa ttgcattgga tgaaaaaaac aggtgctttt ggtcatgatt
      601 atactgcaag gttggtgcaa tcgttgtctg ataaaatcag tactgcaaga tctgaaattg
      661 tagatcatgg acgcacatct actatttcat tagatttacc aatattttct gcatggctag
      721 caaacgaaac aggaaatgtc tctcaagtta tcaattcagc tttagagctt agaacacatg
      781 atcattttcg tacgtgtcga gatgtaatcc gagagattca tgtgacctac gatgaatcgg
      841 ggataagtgc aggaaataaa aaagtaacaa aattactaag tgatttagat aaaatatctg
      901 gagatattaa acgaagctac ggtgttccat caaatcaagg tattcaaggc agttttttga
      961 taaaatgtat aaattcattg acaggactcg tgggtattcc aggtttgcct gataaagagt
     1021 ttgcactgag cactcctgaa tttatgaagt ctaaacaaca taaagcattt tctaccgttt
     1081 tcaaggatgt tactaacgaa ctaacaagca ttgaacgttt aggtgggctt cgggataaat
     1141 tagcgtcgaa ctttactatt gatgattctt attataccga gccaaaaaca gaggatccga
     1201 gattccgtta cgtaagtagt aatt
//
LOCUS       DAZGXS010000374         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGXS010000374 DAZGXS010000000
VERSION     DAZGXS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..196
                     /locus_tag="ACHKG5_005128"
     CDS             <1..196
                     /locus_tag="ACHKG5_005128"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0871021.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIV
                     RGQLVPGGRLGIKITELECA"
     gene            193..882
                     /locus_tag="ACHKG5_005129"
     CDS             193..882
                     /locus_tag="ACHKG5_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0871022.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            1093..1168
                     /locus_tag="ACHKG5_005130"
     tRNA            1093..1168
                     /locus_tag="ACHKG5_005130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1126..1128,aa:Ile,seq:cat)
BASE COUNT          345 a          249 c          322 g          294 t
ORIGIN      
        1 gataaagatt attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa
       61 agcaccactg gatgtgctga cgcatgccgg acttctcata gacgacgagc agtttgatga
      121 aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga
      181 actggagtgc gcatgaataa ccactattta cagtttgtgc gtgagcagct cattatcgcc
      241 accgctgatt tgagtggggc aacaaaaggt cagcttgaag cctggcagga gaatgccatg
      301 ttcgatacag ggcgttacag gcgaaaaaaa atccggtacc gcgatgaagt gactggaaaa
      361 atgataacgc gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggtgacgtca
      421 attcctctgg tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct
      481 cttgaagaac atcataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa
      541 tatcagatcg cgataacaca gtgggcgtgg aatgaattta atactcaatc cggtaccaga
      601 aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca
      661 gtaaaagcag aactttttgg tggggaaggt tatgaataca aggagctggc attactggtg
      721 ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac
      781 atttttcacc gactggatag tgaggcttta ttgtttgtaa tgagaacgcg ttcaaaacaa
      841 aaggcggcat tttcaaagca aagtgttgca aaagtagatt gaaaggcata tatttcatgc
      901 aaatctgata ttttgccgat tttgtacgtg atggcaaaag caaacaaaac ccgcccacaa
      961 gcgggttttt ttgtgccact tatctcggat agacatggtg aatgcgctgg tggaggaagt
     1021 aagggtaatt tttaaccagg tgattcttga atgcttgcaa cattgatttc gtaacgttat
     1081 tatcctgcgc ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggcc
     1141 gctggttcaa atccagcaag ggccaccatc acataccgcc attagctcat cgggatagaa
     1201 cgccagcctt
//
LOCUS       DAZGXS010000375         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGXS010000375 DAZGXS010000000
VERSION     DAZGXS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            49..1143
                     /gene="rfbE"
                     /locus_tag="ACHKG5_005131"
                     /gene_synonym="perA"
     CDS             49..1143
                     /gene="rfbE"
                     /locus_tag="ACHKG5_005131"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP0871023.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFG
                     SKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQY
                     WHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVF
                     HTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGW
                     RGINLPSFPSLSNEQVIYICESINEFYSDK"
BASE COUNT          434 a          180 c          235 g          358 t
ORIGIN      
        1 tagttttaaa aaacatatcg atagacagtt aaatataaga ggatgaaaat gaaatatata
       61 ccagtttacc aaccgtcatt gacaggaaaa gaaaaagaat atgtaaatga atgtctggac
      121 tcaacgtgga tttcatcaaa aggaaactat attcagaagt ttgaaaataa atttgcggaa
      181 caaaaccatg tgcaatatgc aactactgta agtaatggaa cggttgctct tcatttagct
      241 ttgttagcgt taggtatatc ggaaggagat gaagttattg ttccaacact gacatatata
      301 gcatcagtta atgctataaa atacacagga gccaccccca ttttcgttga ttcagataat
      361 gaaacttggc aaatgtctgt tagtgacata gaacaaaaaa tcactaataa aactaaagct
      421 attatgtgtg tccatttata cggacatcca tgtgatatgg aacaaattgt agaactggcc
      481 aaaagtagaa atttgtttgt aattgaagat tgcgctgaag cctttggttc taaatataaa
      541 ggtaaatatg tgggaacatt tggagatatt tctactttta gcttttttgg aaataaaact
      601 attactacag gtgaaggtgg aatggttgtc acgaatgaca aaacacttta tgaccgttgt
      661 ttacatttta aaggccaagg attagctgta cataggcaat attggcatga cgttataggc
      721 tacaattata ggatgacaaa tatctgcgct gctataggat tagcccagtt agaacaagct
      781 gatgatttta tatcacgaaa acgtgaaatt gctgatattt ataaaaaaaa tatcaacagt
      841 cttgtacaag tccacaagga aagtaaagat gtttttcaca cttattggat ggtctcaatt
      901 ctaactagga ccgcagagga aagagaggaa ttaaggaatc accttgcaga taaactcatc
      961 gaaacaaggc cagtttttta ccctgtccac acgatgccaa tgtactcgga aaaatatcaa
     1021 aagcacccta tagctgagga tcttggttgg cgtggaatta atttacctag tttccccagc
     1081 ctatcgaatg agcaagttat ttatatttgt gaatctatta acgaatttta tagtgataaa
     1141 tagcctaaaa tattgtaaag gtcattcatg aaaattgcgt tgaattcaga tggattttac
     1201 gagtggg
//
LOCUS       DAZGXS010000376         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGXS010000376 DAZGXS010000000
VERSION     DAZGXS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            579..>1203
                     /locus_tag="ACHKG5_005132"
     CDS             579..>1203
                     /locus_tag="ACHKG5_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0871024.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASL"
BASE COUNT          388 a          233 c          217 g          365 t
ORIGIN      
        1 cgtaagaggc taacctagat ttgccgttca tagattgagc ctcagattaa tgttaggcgt
       61 cttgcaggac gcgtaatgtt aactggggct tttctctatc tgccgttggt gttcatgccc
      121 gaggcagata gcctcaagca cccgcagcta ttctacttaa cttcccatta cctcgccaat
      181 atgaaatcgg tcagaaagac agacgtccca taaggacaac aacggaaaaa gagattttgc
      241 ttgatgctat ttacctctgc accatgaagt tgcgccaaaa tcccagtggt gataacagga
      301 atttggttgt tggtgattgg ggaaagaagt acgttagact gtgggttagt tcattgacat
      361 gatagttacc tgccttcaat ttcctaagca agcgatgtaa acgataatca atatcaaatg
      421 tattgacagg taaaataata acgatgtacc tttgctgata acatatatca gtcttaaatc
      481 ataatggagg tagataaaat aactaaacac acacaacaaa ttttttatca gtgacatagc
      541 atatcttaag catcctttaa tgctcatggg gagaacgcat gactaaacat atggacattc
      601 attgtcaaac acgcatattt aactcaatta accatgacac caaatataaa atatgtattt
      661 ttactccaaa aaacattaaa aaaaataatg caactcttta tatacttgat gggaatagtg
      721 ccaacaatta catttctgat attctgcctg ttattgatgc actacccaat ccaccagtgt
      781 tagttacact tggttatgaa tcttggaata accttagcat tcatcgccgt gcttacgatt
      841 atactcctga tggtgaaaat gctattgttg ataactctaa accagcatgg atctacttta
      901 ctggtggagg aagccagtct ttccgagaat tattactaac tcagatcatg ccatgggtta
      961 gtactattgc tccgaacagt tccagaatcg gtatatgggg gcactcgcta ggtgctattt
     1021 ttgtgcttga ttgcttaaaa aataattcat gctttaacta ttattatata tctgcaccat
     1081 cacttctatg gcagaatgaa agaatcatca agataattaa agatgatata tcagaatcaa
     1141 agcacactaa aagtatatgc ttgcttaacg gaaatcttag ccttgattac tccgcatctt
     1201 tat
//
LOCUS       DAZGXS010000377         1202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGXS010000377 DAZGXS010000000
VERSION     DAZGXS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..136)
                     /locus_tag="ACHKG5_005133"
                     /pseudo
     CDS             complement(1..136)
                     /locus_tag="ACHKG5_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            286..954
                     /locus_tag="ACHKG5_005134"
     CDS             286..954
                     /locus_tag="ACHKG5_005134"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0871025.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          365 a          254 c          224 g          359 t
ORIGIN      
        1 ctactgatgt atcaacacga ttcaacaata cccgatattt attccatgcc tccagcaacg
       61 atctttcttc ctccgttgcg atttccagat ctacagcatc ctgcagtggc gcaatatact
      121 cactgaattc ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct
      181 gtatcgcagc ataccaggct atttcaatat cgctatgctg cggcagcatt taaccccttg
      241 taattcatcg ccataattga tttaattcac aaataaaact ataacatggt gaaattaatg
      301 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
      361 tggtgggttt tgggagtatc aaatcgtctt gcatggggat gtcctacaaa ggaacacctt
      421 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
      481 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      541 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      601 gatgtttttg aaccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      661 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      721 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg cgatggagtt
      781 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      841 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      901 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
      961 accgcagcac gttcttgcat acgccgtgtc tgcggtttaa tttttactct caaatattgt
     1021 agttaagtac aataatgatt cctgtggcta tatagatgtt tttgtaatta gtccttaaga
     1081 taaaatttct ctatttatcc aagaaacaga cttcattagg aagagtcgta aaaacattaa
     1141 caactaccgc cgtgtaatta aattattttt tagaatgttc tgattgagaa aaacaacgcc
     1201 at
//
LOCUS       DAZGXS010000378         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGXS010000378 DAZGXS010000000
VERSION     DAZGXS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            299..>1186
                     /locus_tag="ACHKG5_005135"
     CDS             299..>1186
                     /locus_tag="ACHKG5_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871026.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFK
                     TNHFKLVYVAFLGKFESSYN"
BASE COUNT          430 a          183 c          210 g          363 t
ORIGIN      
        1 aagcatcctc taagatgctt ttactgttac catgcataca tacccagaac ctttaacaaa
       61 cgataataga ggggagttgt gtataacaac tccctgacct tactaaggtt agcgatgtta
      121 ttcagcatca ttctgtcaaa gatttaattt ctttgaaaaa tactaattct accccttaag
      181 ttaataactt ctgcttgatc gtgtattttg gtaagttgga gtacaatttt ttgattggat
      241 cgtcaataag aaaaagctag aatccttaga aggacataga ttttcatagg gttaagatat
      301 gacgattgcg aaggatagtt cgactgatga aatcattcat ggaaatgatc tccgtggaat
      361 ggatgatttt tacattaaga caacgagttt tgaatgccca tacgagcctt gtaaaattaa
      421 agccacacct tgtagtttta ctatgaaaca tgttaatcaa tcatatttca gatatggcga
      481 taaacataag gatggatgtg gtattcatga tccaagatat aaaaacaatc atacttcaaa
      541 tgacgaaaga aaacataatt cgccacctgc tccggttata tcacttttaa aaattgatgt
      601 gaaaccaaga ggtggtgtta aaaatgccag aaatattaaa aatgaaaatc acaaggatga
      661 aaaaaaaggt aatgaacatc ctgtatcttc atcaagtatt aagccagtcg ttgactatta
      721 cataaataat agcaatcata atgaacagct atcaatccct ccatatggca cgcgatctta
      781 taaagataca tttcaactta ttttttacaa aaacaacata cgttattaca aacctgctat
      841 ttattatggt gtagcccagt ctaatattcg tctgcatgaa gattcagata aacattgtat
      901 tactttcctc gcaagggata agaaaaccca aaaaccattt acgcttgaaa ttgatgtttc
      961 tgactggaat aaaagtcaga aggatgtgtt ttggaaggaa tatgaaaagc aaaggaagga
     1021 agctgatagg tattataagg ggcttaaaga taaaaggaat gctaaaaaat acttgacggt
     1081 tttctttttt gggatgccgg atgaaaatga taagtttcta tttaagacaa accactttaa
     1141 attagtatat gtggctttct tgggtaaatt tgaaagctca tataat
//
LOCUS       DAZGXS010000379         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGXS010000379 DAZGXS010000000
VERSION     DAZGXS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..415
                     /locus_tag="ACHKG5_005136"
     CDS             <1..415
                     /locus_tag="ACHKG5_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HFP0871027.1"
                     /translation="RIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIEL
                     ILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWVTLAESGRGHEI
                     QTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            889..989
                     /gene="ykgR"
                     /locus_tag="ACHKG5_005137"
                     /pseudo
     CDS             889..989
                     /gene="ykgR"
                     /locus_tag="ACHKG5_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          369 a          258 c          227 g          332 t
ORIGIN      
        1 gcggatagag gatacttata aactccttga tcggctcatt tcatccggta tttcgtatat
       61 acatacctcg ctggtcagta ttaacgatag ctaccctgtt gagtcgccaa atggcccccg
      121 aactattgaa ttgattttaa atcatattgc aggcagagtg ccggtaatcg ctgcaggtaa
      181 aatacgcaca ccgtctcagg ctcaagaagc aatttccact ggcctgccac ttgttgcgat
      241 cggtaaaggg ctggtgataa atccagaatg ggtgacttta gctgaatcag gtagaggcca
      301 tgaaattcaa acaaccctta atccacaact tgttcctgaa ttaaccattc cggataaact
      361 atgggatcaa attcaggcat cgaaaggaac tggctggttt ccgttaatgg attaaatatc
      421 tattactctc ccgagagtaa aactatcaaa tcaatgagta accgatacag cctcacaaat
      481 tctttcagat aattaacccg tcactgcggc acgttctctc ataaggcgtg cctgcagtgt
      541 aatctagtat gtatccggca tagttgacgg tttaatagag ctcaattcct cgcccaggtt
      601 accaacaacg atatcttgta taacacccca ggagatacga cattatgatg agatgcgata
      661 tgctcctcag cgccaatcaa cgccaggact gggccagtct tgagatcaga ctcattcccg
      721 gaactgactt caggcgacat atatcaatga cgttggggca ataaaagatg cctgatgtaa
      781 tacatctatt caaatttgtg atacatgtca aaaaattggt tgaccaaact cgttatttat
      841 ataagggcac ttacgaagtg cgctcttttt taaagcgagg aagtaccaat gaaagagaat
      901 aaagtacaac aaatcagtca taaactgatt aatatcgttg ttttgtcgca attgtagaat
      961 acgcctattt atttctccat ttctattaat aacggcaata aactgttcac ttcagtgata
     1021 tttaaaatat gcatcctctc cctttttgta agtaattatt atatccgtgg gagaggaata
     1081 cacgctgtca ggtaatcaat catactgcga taaatcatcg gccagtaaag tggagataac
     1141 ctccattctc gaaaaatcca tactctcagc gaaactatca tcaatc
//
LOCUS       DAZGXS010000380         1182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGXS010000380 DAZGXS010000000
VERSION     DAZGXS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            203..1027
                     /gene="gadX"
                     /locus_tag="ACHKG5_005138"
     CDS             203..1027
                     /gene="gadX"
                     /locus_tag="ACHKG5_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0871028.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          345 a          223 c          244 g          370 t
ORIGIN      
        1 ggattatttg cttactatta atttccctgc gttcttaaac acgtttttgt attataaatc
       61 cagtcatcct gcccgtagaa catcacacat tatcatcctg ttctcccgct tcgtttaaat
      121 ttatttatca atcaatttga cttaagaggg cggcgtgcta cattaataaa cagtaatatg
      181 tttatgtaat attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc
      241 aagacataaa tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga
      301 cctggttttt gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt
      361 agtatcaagg gatacgcttt cattatttct cccgatgctc aaggaggagg cattaaatct
      421 tcatgcacat aaaaaagttt cttcattact cgttcatcac tgtagcagag atattcctgt
      481 ttttcaggaa gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg
      541 taaaagagca ttaatctttg cgttgttatc tgtttttctt gaggatgatc actttatacc
      601 gctgcttctg aacgttttac aaccgaacat gcgaacacga gtttgtacgg ttatcaataa
      661 taatatcgct catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag
      721 tctgttaaag aaaaaattac gcgaagaaga gacatcatat tcacagttgc ttactgagtg
      781 tagaatgcaa cgtgctttgc aacttattgt tatacatggt ttttcaatta agcgagtcgc
      841 agtgtcctgt ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg
      901 gatgacgccc acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc
      961 ggcaagtatt gttgcgcaag ggaattttta cggcactgac tgttctgcgg aaggaataag
     1021 attatagagt tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag
     1081 tctgccgtct ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt
     1141 gctctcagta agttaaatat aacttttact ggaaataaga tc
//
LOCUS       DAZGXS010000381         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGXS010000381 DAZGXS010000000
VERSION     DAZGXS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..253
                     /locus_tag="ACHKG5_005139"
     CDS             <1..253
                     /locus_tag="ACHKG5_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0871029.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHKG5_005140"
     CDS             268..801
                     /locus_tag="ACHKG5_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0871030.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHKG5_005141"
     CDS             798..>1180
                     /locus_tag="ACHKG5_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0871031.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZGXS010000382         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGXS010000382 DAZGXS010000000
VERSION     DAZGXS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..590
                     /gene="espM2"
                     /locus_tag="ACHKG5_005142"
     CDS             <1..590
                     /gene="espM2"
                     /locus_tag="ACHKG5_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP0871032.1"
                     /translation="PMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTIS
                     FSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEER
                     QRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTIA
                     NLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            824..976
                     /locus_tag="ACHKG5_005143"
                     /pseudo
     CDS             824..976
                     /locus_tag="ACHKG5_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1092..>1177)
                     /locus_tag="ACHKG5_005144"
     CDS             complement(1092..>1177)
                     /locus_tag="ACHKG5_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0871033.1"
                     /translation="ADKDSAKTFVQETLKDTWKSADEWFVH"
BASE COUNT          372 a          217 c          278 g          310 t
ORIGIN      
        1 tgccgatgaa tactacaggt atgtctttca gctcttttgg tataagttgc catagagaga
       61 actccttcag gaactctttc cgagggaaga atgatgaggt cgtaaaatgt tcaattggag
      121 aacggactat tagcttttct gttcgcaaat ttagcggcaa tatactggaa acggtgagaa
      181 ggcagagtac taaagatatt gatgagtgga taaaagatga acggatagta tatccctcaa
      241 gggtgatcaa ccaggagatt gataattact gttttcaaaa aaatgcaaaa atttccactg
      301 aagagaggca aagagtcttt tttcttgtga gccaggagaa tcaactaacc cttgatgtta
      361 aggcagcaca aagttctatt aatcatgtaa taatggggag tgcttctttt ggcaagaaaa
      421 tggatgctct ttgtgatggt atgagccggg atgtaaaaaa tcgtacatca gataccatag
      481 caaacttgct tgcggacaag ttttaccaga aacatatcga ttcggatatt gatattgtaa
      541 aactacgaaa tgatatccca gattatttga tgcgtgctat acagggatga ggctagagga
      601 gaggactgct tcagcatcat cggcttcgga ccagtaaaag agacgcagaa aatgcacaac
      661 ggcaccacac gccatgcatg gattcagatt actcatggct caccagtaca gctataaatc
      721 gtaaagaaaa ctacagtacg ttatacacag aaacgtaccg tgattcatta tttattgtac
      781 aggttcatcg cagcgacaat attttgtgat gtttgcgtgg tgcgccccac ttcaggatgc
      841 tgcagatctg gaaattgcaa cggaggaaga aacctcattg ctggaagcct agaaaaagta
      901 tcgggtgttg ctgaaccgtg ttgatacatc aactgcacct gatattgagt ggccggaaga
      961 accagacaca atgtaagcga aaaagaaaaa ccgcagacac gacgtatgca ggacgtgctg
     1021 cggttggctg gtgaactttc gatagtgcga gtattgaatg atttccagcc gttaccgatt
     1081 ttacgtgtta attagtgaac aaaccactcg tcagcagact tccaggtatc tttcagagtc
     1141 tcctgaacaa aagttttagc tgaatcttta tcggcgg
//
LOCUS       DAZGXS010000383         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGXS010000383 DAZGXS010000000
VERSION     DAZGXS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..176)
                     /locus_tag="ACHKG5_005145"
     CDS             complement(<1..176)
                     /locus_tag="ACHKG5_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0871034.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCG"
     gene            complement(401..955)
                     /locus_tag="ACHKG5_005146"
     CDS             complement(401..955)
                     /locus_tag="ACHKG5_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0871035.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1018..>1159)
                     /locus_tag="ACHKG5_005147"
     CDS             complement(1018..>1159)
                     /locus_tag="ACHKG5_005147"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0871036.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          283 a          326 c          218 g          332 t
ORIGIN      
        1 caaccgcact gatagtctgc cagacccgcc ataaccagct gggtcagtat taactggcag
       61 cgttcgcgtg aaaggtaagt attctgcgca atctccccga ctgtcgccgg ttcggtaacg
      121 cttaattcat taaacaccac tctggcggtt tctgtcatat cctgctgttt tagcatgtct
      181 ttttcccttt tccggttaac gtgacatacc aataactctt gtcgaaaaag ccagcaagtt
      241 gaaagaccgg tattagcaac caccagcgcg tttaacgccc cgtgccgttt ttcagtcata
      301 aaaaaacccg caaaaagcgg gctctttcaa atgtccatgt ctgctattcg cctcgcggta
      361 cagctttgcg aagcttaccg gaattgaagc agtttttacg tcaaaaagca ataacttttt
      421 tctctatacc aaaagccata accattggtt tgtacaaaat aaattctgcc acctttagcc
      481 aatgctcaat gcgtctttca caggttctta aactccattc cggatgtgca tcattcagca
      541 attcagccat tttgcgctta gtcatccccc gccccacata acgctgactc agaacattga
      601 gcagcccggg ataacctgcc agtacttcac caataaccct atcgattatt aacgcctctg
      661 aatcggtaca atgtgccagc cagctttttt gattgccgtt gatcatatcc cgcaaaaaag
      721 cctcaagttc aggtttgtcc agacccgctt ttttcatcct ccggagcgcc tcgttaattg
      781 ccgtttttgt cagcttttta gaggtcagta attggttgaa catatttccc gtcttaccgt
      841 cgccaatata cgaccaacgc ccccacatac gcagtttccc ctggatccag acactttcca
      901 gcgttttcag gcgtaaatgc tcaccgcttt tgcctgtaat ttccgggtat atcatattta
      961 tgctcactca ctttcaattt tgtaaatctt cacgcccagc cgcccaccag aaacgagcta
     1021 gccgcgcaca atattgattt catcaaactg ctcgtcgtct atgagaagtc cggcatgcgt
     1081 cagcgcatcc agtggtgctt tcaggatatt gtccaggtca cgacggcgct tatccggtgg
     1141 ctctgcaata atctttatc
//
LOCUS       DAZGXS010000384         1144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGXS010000384 DAZGXS010000000
VERSION     DAZGXS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>1144
                     /locus_tag="ACHKG5_005148"
     CDS             <1..>1144
                     /locus_tag="ACHKG5_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0871037.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETI"
BASE COUNT          312 a          241 c          371 g          220 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaagaaacg
     1141 ataa
//
LOCUS       DAZGXS010000385         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGXS010000385 DAZGXS010000000
VERSION     DAZGXS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            259..495
                     /gene="ycgZ"
                     /locus_tag="ACHKG5_005149"
     CDS             259..495
                     /gene="ycgZ"
                     /locus_tag="ACHKG5_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0871038.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            538..810
                     /locus_tag="ACHKG5_005150"
     CDS             538..810
                     /locus_tag="ACHKG5_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0871039.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSVQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
     gene            839..1105
                     /gene="ariR"
                     /locus_tag="ACHKG5_005151"
                     /gene_synonym="ymgB"
     CDS             839..1105
                     /gene="ariR"
                     /locus_tag="ACHKG5_005151"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0871040.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          396 a          175 c          225 g          344 t
ORIGIN      
        1 cggtgtcttt cttttaatac caaaacataa ctgtttcttt acattgataa aaaatggaaa
       61 aagttgaaca cgagttggcg aaaatttttg tataggttgt cagttaaatg atgcaatatg
      121 ttttatcata acgtattgtt ttatatgcat tagcactaat tgcaaaaaat taatttatca
      181 ttctgtacac atatttcgta caagtttgct attgttacct cacttaacat tgattaacat
      241 ttttaacaga ggcgtagcat gcatcaaaat tcagtgactt tagattctgc gggagcaatc
      301 actcgttact ttgcaaaagc taacttgcct actcagcagg aaactctcgg ggaaattgtg
      361 actgagattt tgaaagatgg acgtaatctg agtcgaaagt cgctttgtgc caaacttctt
      421 tgccgactgg aacaagcgac aggggaagag gaacagaaac attataatgc actaattggg
      481 ttgctttttg aataatgcaa cagcctgatt atttaacagg ttagctatga agtcgttatg
      541 aagacatctg ataatgaaag tataaaatat gaaattactg gccaggcggt gctccagata
      601 ctgcgcatga agataaattt ttcggtgcag actcttatta agcaattact cgtaatgaaa
      661 tcggctgagg aaaatgcttt ccgacgagat ttaattgaca gcataattcg tgattttagc
      721 aacagtgata caggagggcc aaaccgaaga acagcgatag ctgacaataa aagtatgttc
      781 aatggtaaga aaataaacag aatacattaa aatttcataa gtaagatgag aggttaccat
      841 gcttgaagat actacaattc ataatgcaat aactgataaa gcgttatcaa gttactttcg
      901 cagttcgggt aatttgttag aagaagagtc agcagtgtta gggcaggctg tgactaattt
      961 aatgctttca ggcgataatg ttaataataa aaatattatc ttaagtctga tacactccct
     1021 ggaaacaaca agtgatattc tcaaagctga cgtgatcaga aaaacactgg aaatcgtgtt
     1081 gcgatacaca gcggatgata tgtaactttt aattattaag tataagttta tagaaaactc
//
LOCUS       DAZGXS010000386         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGXS010000386 DAZGXS010000000
VERSION     DAZGXS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            4..>1120
                     /locus_tag="ACHKG5_005152"
     CDS             4..>1120
                     /locus_tag="ACHKG5_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0871041.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYT"
BASE COUNT          258 a          297 c          359 g          206 t
ORIGIN      
        1 acaatgggca aaggtggtgg caaggcgcac acgccggttg aggcaaagga caatcttaag
       61 tccacgcaga tgatgagcgt gattgatgcg attggtgaag ggccgattga aggtccggtg
      121 aaggggctgc agagtatcct ggtgaacaaa accccgctga cggacacgga cggtaatcct
      181 gtgatacatg gtgtgacagc ggtctggcgc gccggggagc aggagcagac accacctgaa
      241 ggctttgagt cctccggagc tgaaaccgca ctgggcgtgg aagtgacgaa agcaaagccg
      301 gtgacgcgca ccattacgtc cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag
      361 tcactgttgg agaccacctc aaagggcgac cgtaatccct cttctgtccg actgctgatt
      421 cagttgcagc gtaacggtaa ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc
      481 acctcacagt acctggcgtc ggtgattctg gagaatctgc ctccccggcc ctttaacatc
      541 cggatggtcc gggagacggc ggacagcacc acggaccagc tgcagaataa gacgctctgg
      601 tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg
      661 ctgcaggtgg atgcggagca gtttggcggt cagcagatga cggtgaacta ccatatccgc
      721 ggtcgcatca tccaggtgcc gtcaaactat gacccggaaa aacgcacgta cagcggcatc
      781 tgggacggca gcctgaaacc ggcatacagc aacaacccgg cctggtgcct gtgggacatg
      841 ctgactcacc cgcgctacgg catgggaaaa cgcctggggg cggcggatgt ggacaagtgg
      901 gcgctgtatg ccattgcgca gtactgcgac cagatggtcc ctgatggttt cgggggcaca
      961 gagccgcgga tgacctttaa tgcgtacctg tcacaacagc gtaaggcgtg ggacgttctc
     1021 agtgatttct gctcggcgat gcgctgtatg ccggtatgga acggccagac gctgacgttc
     1081 gttcaggaca gtccgtcgga tgtggtgtgg ccgtacacca
//
LOCUS       DAZGXS010000387         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGXS010000387 DAZGXS010000000
VERSION     DAZGXS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..527
                     /locus_tag="ACHKG5_005153"
     CDS             <1..527
                     /locus_tag="ACHKG5_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0871042.1"
                     /translation="AGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNSNE
                     ASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDAEA
                     EKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNRVD
                     TTVAADVEWPVAPQ"
     gene            complement(571..>1107)
                     /locus_tag="ACHKG5_005154"
     CDS             complement(571..>1107)
                     /locus_tag="ACHKG5_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFP0871043.1"
                     /translation="FGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQRNRNFLFWWRLA
                     NEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVDAGE
                     NLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKD
                     IPSNCTYITKKSGVVLYK"
BASE COUNT          318 a          235 c          226 g          328 t
ORIGIN      
        1 tggccggaac taatgaattt attggtgaag gtgacgcata cattccgcct catacaggtc
       61 tgccagcaaa cagtaccgat attgcaccgc cagatattcc ggctggcttc gtggctgttt
      121 tcaacagtaa tgaggcatcg tggcatctcg ttgaagatca tcggggtaaa acggtttatg
      181 acgtggcttc cggcgacgcg ttatttattt ctgaactcgg tccgttaccg gaaaatgtta
      241 cctggttgtc gccggatgga gagtgtcaga agtggaacgg cacatcctgg gcgaaagatg
      301 cagaagcaga aaaactgttt cgggtacggg aggcagaaga aacaaaaaac agcctgatgc
      361 aggtagccag tgagcatatt gcgccacttc aggatgccgt agatttggat attgcgacgg
      421 aggaagaggc atcgttactg gctgcatgga agacatatcg ggtattgttg aatcgtgttg
      481 atacaacagt agcagcggat gttgagtggc cagtcgcccc acaataaaaa gaaaaagcca
      541 tcgacagaaa tatcgatggc tttatgtact ctatttatac aatacaacac cactcttttt
      601 agttatatat gtgcagtttg atggtatatc tttatttata aaagacattg cacctatttt
      661 tacattatcc ccaattttac gtgataatcc aatgatgcaa caattagctc cgatatcaac
      721 gttactacca atttttactc ttgaactagg catatcacca tctatctgtc caatggtagt
      781 attctgtcgt aataccagat tttcacccgc atcaacagca aaatgaacaa caattccagc
      841 atgatgggga attgtcaacc cttttccaat atttgcgccc aatcctattt cacaaccaaa
      901 cttgttaatt attttactgt ttaacttttt ggctgctttc ttatgtaatt tattaccatt
      961 aatatacatt tcgttagcca accgccacca gaaaaggaaa ttccggttac gctgcttttt
     1021 ctctcttaaa agcctccaga tatccatacg tttccgccga attacttcat gtttccagaa
     1081 gttttttaaa ttagtagagt ttccaaa
//
LOCUS       DAZGXS010000388         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGXS010000388 DAZGXS010000000
VERSION     DAZGXS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..324
                     /gene="escR"
                     /locus_tag="ACHKG5_005155"
     CDS             <1..324
                     /gene="escR"
                     /locus_tag="ACHKG5_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP0871044.1"
                     /translation="EKNTEKDNVEFFERAAQKKLGNETILKKDSLFILLPAFTMGQLE
                     AAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEF
                     LLVVN"
     gene            324..593
                     /gene="escS"
                     /locus_tag="ACHKG5_005156"
     CDS             324..593
                     /gene="escS"
                     /locus_tag="ACHKG5_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP0871045.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            593..>1097
                     /gene="escT"
                     /locus_tag="ACHKG5_005157"
     CDS             593..>1097
                     /gene="escT"
                     /locus_tag="ACHKG5_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP0871046.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALI"
BASE COUNT          315 a          189 c          203 g          390 t
ORIGIN      
        1 gaaaaaaata cagagaaaga caatgtagag ttttttgaac gtgcagctca aaaaaaattg
       61 ggtaatgaaa ctatcttaaa aaaagactct ctatttatac tgttaccggc tttcacgatg
      121 gggcagcttg aagctgcatt caagataggt tttttgctct atttaccctt tattgcgata
      181 gatttgatca tttccaatat cttattggcc ttgggtatga tgatggtatc gccagtaaca
      241 atttcgattc cttttaagat cttattattc attcttgttg gaggctggca aaaattattc
      301 gaatttctgt tggtggtgaa ttgatggata ctggatattt tgttcaatta tgtgtgcaaa
      361 cgttctggat aatatttatc ctctcattgc ctacagtcat agcggcctct gttatcggta
      421 ttattattag tctggtccag gctataacgc agttacagga tcaaacattg ccttttttgc
      481 taaaaataat agcagtgttt gctacgcttg ccctgactta tcactggatg ggaacaacaa
      541 tcatcaactt ctcgtcgata atatttgaaa tgattccgaa ggtgaacggc taatgaatga
      601 gataatgacg gtcatagtat catcatttta ttgtatactc aggccgctgg ggatgtttat
      661 tatcctacca atattttcaa caggtgtttt actcagtaac tttattcgca actcaattat
      721 gattgcgttt acgcttccga tcatagtaga gaactataca ttttcggaaa aattgccatc
      781 tggaatcttt cagttgacag gaatagcgct aaaagaaata agtattggtt ttttcattgg
      841 gttatcattt actattcttt tttgggcaat agatgcggct ggacagatta ttgatactct
      901 aagaggctca acaatatctt caatttttaa cccgtccata agtgattcat cttctatcac
      961 tggcgttatc ttgtaccaat ttatctctgt gatctttgtt attcatggtg ggatacaaag
     1021 catcctagat aagctatatt tatcctacga gatattacca ttacaagccg atattgcatt
     1081 caatcgtgct ttaatag
//
LOCUS       DAZGXS010000389         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGXS010000389 DAZGXS010000000
VERSION     DAZGXS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..881
                     /locus_tag="ACHKG5_005158"
     CDS             <1..881
                     /locus_tag="ACHKG5_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0871047.1"
                     /translation="LMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNFFPPDDKGER
                     EQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLGLMTGARVNE
                     IAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELGFIEYVDALK
                     AAGYDRLFPELKPHKTKGYGRLVSAWFNESLLAGRLKLERDRSKSFHSFRHSVSTLLK
                     EKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEIARFNIPDGL
                     DAVRDALRRKRGKQTG"
BASE COUNT          297 a          211 c          285 g          298 t
ORIGIN      
        1 aactgatgac agaagagtct gtcaaaaagt atatcaacgg cctttatggt gcaatggagt
       61 gggctgttga tgatggtaag tttctgaaat cgccatgcga caactttttc cctcccgatg
      121 acaaaggtga gcgagagcag gatcacactg acatatttga accgcatgaa attaaggcaa
      181 ttttttcgca accgtggttt gtcgctggaa ctgttgaacg taatgcgcaa gggcgattcc
      241 atcaatattg cccgtttcac tattgggcgc cgttgttggg cttgatgacg ggggcaaggg
      301 ttaacgagat tgcacagtta atgctggacg atgttctggc agatgacggc gtttattacc
      361 tgaaccttga aagcgatagc gaaaacggaa agaaactaaa aaacgccaat tcccgccgca
      421 agattccggt tcattctacg ctgattgaac tcggttttat cgagtatgtg gatgcgttga
      481 aagctgccgg gtatgaccgt ctttttcccg agcttaaacc acataaaacc aaaggctatg
      541 gtaggctggt ttccgcatgg ttcaatgaat cattgcttgc gggtcgatta aaacttgaaa
      601 gagacagaag caaatctttc cactctttcc ggcattctgt ttcaactttg cttaaagaga
      661 agggggttag ttcggaactg cgtgggcagc tacttgggca tgtgcgaggc aaaacagaaa
      721 ctgaagtgcg atacagcaaa gatttaaaac cggttcacat ggttgaggtt gtcgaaaaga
      781 ttgatttttc tttgcccgag atagcgagat tcaacattcc tgatgggctg gatgctgtaa
      841 gagatgcgct gcgaagaaag cgtggcaaac aaacaggttg agtcagttac tattcagttg
      901 agttatgctc ttgaatgcgc tgggacgctt cctggcgcat tttttattaa aggtcgtgga
      961 tttaatagtg ccaaatttgg agacgcttaa aatgcgtctg gtgagacttg atttgcatta
     1021 gctcagaatt gatctgacgg ttttctctga cagcatacaa tcaagtctaa cagtccgcct
     1081 tgagcgagga g
//
LOCUS       DAZGXS010000390         1083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGXS010000390 DAZGXS010000000
VERSION     DAZGXS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(94..170)
                     /locus_tag="ACHKG5_005159"
     tRNA            complement(94..170)
                     /locus_tag="ACHKG5_005159"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Arg,seq:tct)
     gene            complement(271..346)
                     /locus_tag="ACHKG5_005160"
     tRNA            complement(271..346)
                     /locus_tag="ACHKG5_005160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Ile,seq:cat)
     gene            complement(375..>1083)
                     /locus_tag="ACHKG5_005161"
     CDS             complement(375..>1083)
                     /locus_tag="ACHKG5_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0871048.1"
                     /translation="VVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMHYG
                     KVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVT
                     DMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSE
                     SLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHR
                     YYGFPPDRYVSRQGLDY"
BASE COUNT          313 a          271 c          241 g          258 t
ORIGIN      
        1 taagctcacc cccgaaaggc tctgtggttg gtatgcgccg ggcgtggcgc agatacaaaa
       61 aaggcccgcc gaagcgagcc tggaaaataa gtgtggcgcg ttgtagtgga gtcgaaccac
      121 tgaccgattg cttagaaggc aattgctctg tccggctgag ctaacaacgc agaataccga
      181 taatggaccg ccatcgggga cccggccccg caccaacaac cctgttatgg tgtcgtctgc
      241 tcttcctgat aagctaatgg cggtttgtga tggtggccct tgctggattt gaaccagcga
      301 cctggcgatt atgagtcgct cgctctcacc actgagctaa agggccgagc caaaaaataa
      361 taatcagatg aaaatcaata atcaagccct tgcctggata catatctgtc tggcgggaag
      421 ccataatagc ggtgaaatac agaaataaag taggaccggc ttgaataacc acatttttct
      481 gctacagcct gtccatatcc ataccgggaa cataacatat ttacagcaac acgcatccgc
      541 tcttccagca acaagcgact gaacatgccc ccttcatttt tcagttttgt ctttaacaaa
      601 ctctcactca tatgcaactg tagagcaatc gcaccaagcg tccagcttgc tgatatatct
      661 gtctgaatta tcgccctgac tttggcactt atgctggata aacatccact taaaaacaat
      721 gacatccgtt catctgtttc aaacagagac aggcaggcca tcataagaaa catatccgtg
      781 acctctccgg aaaatccctg gctggtaatt aaagccgcag ccaacgcagg attgttgggt
      841 tccagcaaca ggtaaagcgg aatgtcagtc agacgagtcc tcgtcagctt atgctgactt
      901 tccagatatt gacttacgac ggattcgctt atatcgacaa ttttaacttt gccataatgc
      961 ataaggaaaa gctccctgat gcatttggtg gccagaacga ctgagcctgg cttaagtgac
     1021 aacgtatcct tttcaagaaa aatattaatt ggggagcaaa ccatgataac tgaacagaca
     1081 aca
//
LOCUS       DAZGXS010000391         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGXS010000391 DAZGXS010000000
VERSION     DAZGXS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            167..538
                     /gene="ler"
                     /locus_tag="ACHKG5_005162"
     CDS             167..538
                     /gene="ler"
                     /locus_tag="ACHKG5_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP0871049.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            553..771
                     /gene="escE"
                     /locus_tag="ACHKG5_005163"
     CDS             553..771
                     /gene="escE"
                     /locus_tag="ACHKG5_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFP0871050.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            784..>1076
                     /gene="cesAB"
                     /locus_tag="ACHKG5_005164"
     CDS             784..>1076
                     /gene="cesAB"
                     /locus_tag="ACHKG5_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFP0871051.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMNQRN"
BASE COUNT          442 a          151 c          222 g          261 t
ORIGIN      
        1 aaagagaata ataacatttt aaggtggttg tttgatgaaa tagatgtgtc ctaatttgat
       61 agataaacgt tatctcacat aatttatatc atttgattaa ttgttggtcc ttcctgataa
      121 ggtcgctaat agcttaaaat attaaagcat gcggagatta tttattatga atatggaaaa
      181 taattcacat acaacaagtc catacattca gcttatagag caaattgcag ttctacagca
      241 ggaagcaaag cgactgcgag agcaggaagt tcaaagtgta attgagtcga ttcagaagca
      301 gattacttat tacaatataa ccttacaaga gctgggatat actaatgtgc ctgatgatgg
      361 actcgctcgc cggaactcat cgaaaggtgt ttactaccgc aatgaagaag ggcagacctg
      421 gtcgggcgta ggccgacagc cacgctggct taaagaagca ctgttgaatg gaatgaagaa
      481 agaagatttt cttgtgaagg acactgaaga agaaataata ccgctgaaaa atatttaaca
      541 tgaaataatt aaatgataac gataactgag ctggaagatg aaataataaa aaataaagaa
      601 gccgcaaatg tttttattga aaaaataaac gacaaaaaga acgaaatcca tgaaaaaatg
      661 aaacaccctt tggataaagt tacctacaat gaagcaaagg aactgctcat tgcgtgtgat
      721 gcggcaatta ggactataga gataatgcga atcaggatta ataataaata gaggatgagt
      781 attgtgagcc aaacaagaaa taaagaacta ttagataaaa aaataagatc agaaattgag
      841 gcgataaaaa aaataatcgc cgaatttgat gtcgtaaaag aaagcgttaa tgagttaagt
      901 gaaaaagcaa aaacagatcc tcaggcggca gaaaaattaa acaaattaat tgaggggtac
      961 acatatggtg aagagcgaaa attatatgat tctgccctaa gtaagattga aaaactaata
     1021 gagacactga gtccagcaag atctaaaagc caatcaacaa tgaatcaacg gaatag
//
LOCUS       DAZGXS010000392         1050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGXS010000392 DAZGXS010000000
VERSION     DAZGXS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..516)
                     /locus_tag="ACHKG5_005165"
     CDS             complement(<1..516)
                     /locus_tag="ACHKG5_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0871052.1"
                     /translation="MIYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNA
                     YSVVSHNQFFEKVWSDSSADVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGF
                     KINEEISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVCFFAAW
                     GIVYISSYFFDM"
BASE COUNT          323 a          180 c          181 g          366 t
ORIGIN      
        1 catatcgaaa aaataagaag atatatacac tatcccccat gccgcaaaaa aacaaactat
       61 aagtaatgta ttctttgtgg taaatatacc ttttgcaatg tgatttttat ttatattttc
      121 agtgtgggta ccattctctt cactctcaac actttcattc tcattgtgat tattgtcgag
      181 aacagttatg ctgatttctt cattgatttt gaagccttta cggggcaccg ttctgataac
      241 atcattaaat atcgtcgctg agctgatttc acgaaaagaa cgtcttatca tcgatatatt
      301 ttgatataaa gtgttggttg cgacatcggc actgctatcg ctccagactt tttcaaagaa
      361 ttgattgtgt gacacgacac tataagcgtt atctaaaagt aactttaagc attttcccgc
      421 aggataagtg agtatcactt cctctgatgt aaggagtgat gttaacttat tagatgatga
      481 gttgaatctt acttcatcat ttataatgta gatcattaca ttttatacct tagttgtgta
      541 ttacaaatcc agtattactc agaaaataaa ggcaagaaca ttaagtgcac tatagtataa
      601 tttcctaaaa atgcaaatct gaattatctg atttttaaga ataatcttga tttaattgaa
      661 attcttaaac gatgtatatt tctattaaat gctttctgca gtgatgtgaa ttaggggttt
      721 ttctgcatgt actgataaaa actgcgtatc cccgttatag ggaaagaacg ggacgcatcc
      781 tccacccatg acactaaccg tgtgattggt agttccctgc tgtgcacttc tggtgaaata
      841 gaagaagctc tatgtgccga ccggtactgt cttttatccg attatttacc agtgacacct
      901 tgatatgagg ttatatctat tattgctagg aggtaatata tgcttatgtt attatccaaa
      961 gcgccaatag taatttagat aaagattata gatcagcaag caaatccagt ctgttttttt
     1021 gtgcgtactg cataacatct atgatgacta
//
LOCUS       DAZGXS010000393         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGXS010000393 DAZGXS010000000
VERSION     DAZGXS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..75
                     /locus_tag="ACHKG5_005166"
     CDS             <1..75
                     /locus_tag="ACHKG5_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871053.1"
                     /translation="TSYLWVQGQKVVPAVVFSNFTLVD"
     gene            95..>1046
                     /gene="nleL"
                     /locus_tag="ACHKG5_005167"
     CDS             95..>1046
                     /gene="nleL"
                     /locus_tag="ACHKG5_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP0871054.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSG"
BASE COUNT          313 a          168 c          212 g          353 t
ORIGIN      
        1 acatcttatt tgtgggtaca gggacagaaa gttgtcccgg cagttgtgtt ttctaatttt
       61 actttagtgg actaagtaaa aaggagtgag ataaatgctg cccactacaa atatctctgt
      121 aaattctgga gtaatatctt ttgaaagtcc tgtagattca ccatctaacg aggatgttga
      181 agttgccctc gaaaagtggt gtgctgaggg agaatttagc gaaaatcgtc atgaggttgc
      241 atcaaaaata cttgatgtta taagtactaa tggagagact ttatcaatca gtgagccaat
      301 aacaacatta ccagacttgc ttccaggttc tctgaaagaa ctggttttga atggatgtac
      361 agagcttaaa tcaataaact gcttaccccc caacttatct tcattaagta tggttggatg
      421 ctcatcatta gaggttataa attgcagcat acctgaaaat gtcattaatt tatctttatg
      481 ccattgtagt tctttgaaac atatagaagg ttcctttcct gaggcactca gaaattccgt
      541 atatttaaat ggctgtaatt cattaaatga atcgcaatgt caattccttg catatgatgt
      601 cagtcaaggc cgtgcctgcc tgagcaaagc tgagcttact gctgacttaa tttggttgtc
      661 agctaaccga acgggtgaag agtctgctga agaattgaat tactctggat gtgacttgtc
      721 aggtctaagt cttgtagggc tgaatttatc atcagtaaat ttttctggag cagtgcttga
      781 tgatacagat ctcaggatga gtgatttgtc tcaggctgta ttggaaaact gttcttttaa
      841 aaactcgatt ttgaatgaat gtaatttttg ttatgctaat ttatctaatt gtattattag
      901 ggctttgttt gaaaactcta atttcagcaa ttccaatctt aaaaatgcat catttaaagg
      961 atcttcatat atacaatatc ctccaatttt gaacgaggct gatttaacag gagctattat
     1021 aattcctgga atggttttaa gtggtg
//
LOCUS       DAZGXS010000394         1042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGXS010000394 DAZGXS010000000
VERSION     DAZGXS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            78..>1042
                     /locus_tag="ACHKG5_005168"
     rRNA            78..>1042
                     /locus_tag="ACHKG5_005168"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          275 a          225 c          328 g          214 t
ORIGIN      
        1 acgaaaaatg aataccaagt ctcaagagtg aacacgtaat tcattacgaa gtttaattct
       61 ttgagcatca aacttttaaa ttgaagagtt tgatcatggc tcagattgaa cgctggcggc
      121 aggcctaaca catgcaagtc gaacggtaac aggaagaagc ttgcttcttt gctgacgagt
      181 ggcggacggg tgagtaatgt ctgggaaact gcctgatgga gggggataac tactggaaac
      241 ggtagctaat accgcataac gtcgcaagac caaagagggg gaccttcggg cctcttgcca
      301 tcggatgtgc ccagatggga ttagctagta ggtggggtaa cggctcacct aggcgacgat
      361 ccctagctgg tctgagagga tgaccagcca cactggaact gagacacggt ccagactcct
      421 acgggaggca gcagtgggga atattgcaca atgggcgcaa gcctgatgca gccatgccgc
      481 gtgtatgaag aaggccttcg ggttgtaaag tactttcagc ggggaggaag ggagtaaagt
      541 taataccttt gctcattgac gttacccgca gaagaagcac cggctaactc cgtgccagca
      601 gccgcggtaa tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca
      661 ggcggtttgt taagtcagat gtgaaatccc cgggctcaac ctgggaactg catctgatac
      721 tggcaagctt gagtctcgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag
      781 agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg
      841 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc
      901 gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc
      961 tggggagtac ggccgcaagg ttaaaactca aatgaattga cgggggcccg cacaagcggt
     1021 ggagcatgtg gtttaattcg at
//
LOCUS       DAZGXS010000395         1033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGXS010000395 DAZGXS010000000
VERSION     DAZGXS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>1033)
                     /locus_tag="ACHKG5_005169"
     CDS             complement(<1..>1033)
                     /locus_tag="ACHKG5_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0871055.1"
                     /translation="AVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIA
                     ASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELW
                     TQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSM
                     WAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMINPANGNTKPMFVGQG
                     DQIFMNEVFLKYLTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGTLNNVTI
                     NENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVI
                     PAVAFSGAKH"
BASE COUNT          230 a          292 c          244 g          267 t
ORIGIN      
        1 catgtttagc gccgctgaat gccaccgccg gaataacaat ctgccggtca aacggctgat
       61 cgtcataaac cctgacggta atggtccctg atggccaccg ttccggtgca cgggagtccc
      121 gggggaaagc tttgcccact gttttaacga gatcgccttc aatctggttc gcggacagtt
      181 ttcccagaac ccgacagttc tcgttaatcg tgacgttgtt gagcgtcccg gagttcgcat
      241 tcacactgcc actgatatcc gcatttttag cggtcagctt tccgtctgat gtcagggaaa
      301 atgccggagg attgccgcca ctggtaatgg tgggagccgt cagatatttc aggaacactt
      361 cattcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg ccattcgcag
      421 gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatg ccgtcagagg
      481 tgttctcaat accggcaccg atacccgcaa tataaaggcg tccgtcctgc atctgctgca
      541 gcttcactgc ccacatgctg ttcaggttat tatttgtatc aacctgaacc ttctgtatct
      601 gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg atttcattac
      661 tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga ccgttatcaa
      721 tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg aaaaaatcca
      781 gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc gatttgccaa
      841 cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata ttgatactgg
      901 cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc tgcctgatat
      961 cggtaatccg cgtttccgaa aaccagaact caaactgtac cgtcgggtca tacaccgcaa
     1021 gacgcgggac cgc
//
LOCUS       DAZGXS010000396         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGXS010000396 DAZGXS010000000
VERSION     DAZGXS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..1002
                     /locus_tag="ACHKG5_005170"
                     /pseudo
     CDS             1..1002
                     /locus_tag="ACHKG5_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          249 a          230 c          317 g          206 t
ORIGIN      
        1 cggggaaaac ggcgattctg agcaacgggg cgaaatataa ccccacgacg ttttcaccgg
       61 tggatgcgca gacggtggaa caactgaaaa tgaccgctga aattgtctgt tcggtgttcc
      121 gtgtcccggc ctacaagatt ggtgtgggac aacctccctc cagtgataac gtggaggcgc
      181 tggagcagca gtattattcc cagtgtctgc agacgctgat tgagtccatt gagctgttac
      241 tggatgaggc gctggaaacg ggggaaaacg agagcacgga gtttgacgtc accacgctgc
      301 tgagaatgga cagcgaacgg cgcatgaaaa cgctgggtga gtcggtgaaa aatacgctgc
      361 tgacgcccaa tgaggcccgt aaacgtgaga acctgccgcc cctggccggc ggtgatgcac
      421 tgtatcttca gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat gcccgtgagg
      481 atccgttcgc gtcgtccggg aaaacagtct cagcacaact gcctgatggc gcatctgacg
      541 gtaataaggc aatcagtgaa acagagcatg atgcggtgaa agcgatgttc agggggatac
      601 tgcgaaaatg acggaacggg aactgtccat tattcgtgca ctgggcgaag aattcgccat
      661 ggtgctggcg gatttacagc gtacatttga ggagaaaata gccgcgcagg cacaaacgtt
      721 tgaagaaaaa ctggcttctc agtctgtggt attacagaag tgcgtgacgg gtgatgatgt
      781 gcgtccgatg cttgagcaga tggtgaaaga ggcagtgagc catattcctg ttccgcgcga
      841 cggtcgtgac tacgatccgg atgttctgca gaaggcggtg aatgatgcgg ttgcgaatat
      901 tccggtaccg gcagacggca aaagtatcac cccggatgat gtgcgtccga tgcttgagca
      961 gatggtgaaa gaggcagtga gccatattcc tgttccgcgc ga
//
LOCUS       DAZGXS010000397          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGXS010000397 DAZGXS010000000
VERSION     DAZGXS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..120
                     /locus_tag="ACHKG5_005171"
     CDS             <1..120
                     /locus_tag="ACHKG5_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HFP0871056.1"
                     /translation="TSDFFIKVDTSINFYVTRQNDYNYVFSTGRTPRFICISH"
     gene            complement(222..>994)
                     /locus_tag="ACHKG5_005172"
     CDS             complement(222..>994)
                     /locus_tag="ACHKG5_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0871057.1"
                     /translation="RWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALM
                     RWQREDGKNISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAE
                     HLAHPDFVADVLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDD
                     FGTGYCSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGV
                     STSHQAEYLIHNNVTLVQGYLYAKPMKATEFYQWYVNR"
BASE COUNT          293 a          209 c          159 g          333 t
ORIGIN      
        1 acttctgatt ttttcataaa agttgacacc tcaattaatt tttatgttac acgtcaaaat
       61 gattacaact acgtattctc tacaggcagg acaccacgct tcatatgtat ttcccactaa
      121 cagcattgtt gctcctggcg tttgtcacca gttaataacc ttctgatggc tttgtttata
      181 ttttaaggca ataaaaaacc attccaatat tggttaatta tttatctgtt tacataccat
      241 tgataaaact ctgtcgcttt cattggtttt gcatataagt atccctgaac taatgtcacg
      301 ttattatgaa taagatattc cgcctggtga gaggtactga caccttctgc aatcgttatc
      361 aaattgagtc gattactcag cttaataata atatctaaaa caggcgtttc gtcttccgct
      421 gtagtgagcg tgtctataaa gcttttatct attttaagat aatcaacaga caacttttgc
      481 agaaggccaa gggaacaata tcccgtgcca aaatcatcaa ctgcaaattc acagccatgg
      541 ctccttaatt catttatttt ctgtaatgca tgatcagtat cttcaaccag attgcgttcg
      601 gtaatctcaa ggacaatgtt aaatttatca gatattgcat tttttatatg taaaacatca
      661 gcaacaaaat caggatgtgc aagatgttca gcggcaatat ttatcccaag gtgaaacggc
      721 tttttcactt tccagctttg tacatcctga ataatcagtt caaagagatg cttagttaac
      781 ggtataatta aattttcctc ttctgcagcg cggataaaaa ctaccggaga tatattttta
      841 ccatcttcgc gttgccaacg cattaacgcc tcaaccccca gacacgcttt ggttgtcgtg
      901 tcacatactg gttgataatg cacagaaaat tctccggatg acattccttt cttaatttct
      961 tctgccaggg acattttccg agatatccag cgct
//
LOCUS       DAZGXS010000398          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGXS010000398 DAZGXS010000000
VERSION     DAZGXS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>972
                     /locus_tag="ACHKG5_005173"
     CDS             <1..>972
                     /locus_tag="ACHKG5_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFP0871058.1"
                     /translation="SLSEIHRTLTNSTSFADISIPLLQTICPSITTIIKKTEYYGHQL
                     TNAMTVASVVKPYDFSNLGAINSIDKSVSDVPALHTIVEQAKYNLLSWNDFYNTHASI
                     WDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGGGYQKLRHNIDTAS
                     TLYQTKYNDNLKLSNRDDFFLRKTQRIITMSNELGNNRLKNAQLEVLELKDPILTEGI
                     LYQRRISSLLITTEYHSLALQQISSFWRVTDPNFGHCDFHSLAQALTFIKNITSNRNF
                     SSLYGSGIVKIYFSESLNNWKYIKLPLVQTGSLLRDIYLTTPEKLST"
BASE COUNT          346 a          169 c          152 g          305 t
ORIGIN      
        1 atcgctgagt gaaatacata gaactttaac aaatagtact tcatttgcag atatttctat
       61 accattattg cagaccatat gtcccagcat aacaacaata ataaaaaaaa cagaatatta
      121 tgggcatcag ttaacaaatg ctatgactgt tgcttcagtg gttaaaccat atgactttag
      181 caatcttggt gccataaata gtatagataa atccgtttct gatgtgcctg cattgcatac
      241 aattgttgaa caagcaaaat ataatttact ttcatggaat gatttttata acactcatgc
      301 gtcaatatgg gatacaatag caagacagca taaaagtaca aatatcgaat ttcacccaca
      361 aagtcttctt tttgatagag attcgaaagg aaagtgcctt ggtttatctc ttctgtatct
      421 tgatacaggg gggtacggag gcggatatca aaaactgagg cacaatattg acactgccag
      481 cacattatat caaacaaaat acaatgacaa tctaaaatta tctaaccgtg atgatttctt
      541 tttaagaaaa acacagcgta tcattacaat gtccaatgag ttaggtaata acaggttaaa
      601 aaatgcacag ttagaggttc tggaattgaa agaccctata ctaactgaag gcatattata
      661 ccagcgtcgt atatcatctc ttttaataac aacagaatat cattcacttg cattacaaca
      721 aatatcttct ttttggagag taactgatcc taattttggt cattgtgact ttcattcctt
      781 agcacaggct ttaacgttta taaaaaatat aacgagcaat cgtaactttt catctttata
      841 tggaagtggc attgttaaaa tttacttcag tgaatctctt aataattgga agtatattaa
      901 gctccccctt gttcagactg gttctttgtt aagagacata tatttaacaa ccccagaaaa
      961 attatccaca ag
//
LOCUS       DAZGXS010000399          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGXS010000399 DAZGXS010000000
VERSION     DAZGXS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            43..843
                     /locus_tag="ACHKG5_005174"
     CDS             43..843
                     /locus_tag="ACHKG5_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871059.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          307 a          177 c          211 g          258 t
ORIGIN      
        1 taaatattct aatcattatg acaggcgagg gagtgtccaa ttatgaattc aaaaaagctt
       61 tgttgcatat gtgtgttatt ctcgctgctt gcaggatgtg cctctgaatc ttctattgat
      121 gaaaagaaga aaaaagctca agtcacacaa agtaatatta ataaaaacac tccccagcaa
      181 ctgacagaca aagatttatt cggtaatgaa accactctgg ccgtatccga agaggatatt
      241 caagcagcgc ttgatggaga tgagttccgc gttcccctta attctcctgt aatacttgtt
      301 caatccggca accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa atattatact
      361 gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa taaaaacaaa
      421 gataaaaatg agaacgagga tgttgcccgt gctgagaata tgaactggat gcaggcactg
      481 cgttttgtgg ctgcaaaagg acatcagaaa gcgattattg tttaccagga tatgttgcag
      541 acaggaaaat atgactctgc gctgaaatca acagtatggt ccgactataa aaatgaaaaa
      601 ctcactgacg ctatctccct gcgctacctg gtacgtttca cgctggtgga tgtggcaaca
      661 ggtgagtggg ctacctggtc gccggtgaat tatgaatcga gagtaatttt tccgccggct
      721 ggaataacaa aaacgagcaa taaagaacta tcaaatgacc atgttacgga agcgcaactg
      781 tttaatttga aacaaaaaac atatgtatcg atggtgaaag acttggtgag tcgattccag
      841 taatcgttat tgttatgctg ccgcctgtag tttagggtgg cagcaaacag gagttgagta
      901 tttctataat gtaaatatga ttgtttttat aaagttataa tatatattac cag
//
LOCUS       DAZGXS010000400          946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGXS010000400 DAZGXS010000000
VERSION     DAZGXS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            255..440
                     /locus_tag="ACHKG5_005175"
     CDS             255..440
                     /locus_tag="ACHKG5_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP0871060.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            681..>946
                     /gene="nleB"
                     /locus_tag="ACHKG5_005176"
     CDS             681..>946
                     /gene="nleB"
                     /locus_tag="ACHKG5_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP0871061.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIG"
BASE COUNT          326 a          165 c          170 g          285 t
ORIGIN      
        1 aaaggtagca gaatacctaa gcgagttcag cggaaaacat ttcctcgatg tatataatta
       61 ttattccaat tgaaaaaaat ataaagtatc tatcacggca ctctcagttg agtatcacag
      121 gcacatgtat tttagccccc tgagtctaca gacaaccaat attacttaaa taagtgataa
      181 tcttaatgct gttttttaga acagatgatt gatatttttg ggccggagaa acgcagacag
      241 gctttgttga ataaatcgag cttttgctga gtttaaggat cagatcactc attttcctgc
      301 aaaagctcat tgggcgaaac aatctgactc agagaacccg gattaaacgt ctggcccgca
      361 gaatcatcta tttctcacgc actgtagtac gtaataaaaa agtcatcggt acttttatca
      421 aaaaacatat attctattaa ttattgccat cacctttttc ttattgacaa cattactctg
      481 ctgattgtta aaatctaacc tgtattcagg gttacgattc tatacaatag aacagggagg
      541 ggaaaggatg tcatcgttaa tgagactttt tgttaatgtc aacaatgcta tgaatttatt
      601 ttagaaaaac aattaagctg acgtagtgtt ccgtctaaat gacggggcga tattaacatg
      661 attagaacaa gaggaatttt atgttatctt cattaaatgt ccttcaatcc agcttcagag
      721 gaaagacagc tttatcaaat agtacacttc tccagaaagt ttcttttgct ggaaaagaat
      781 atcctctgga acctattgat gaaaaaaccc ctattctttt tcagtggttt gaagcaaggc
      841 cagagcgata cgaaaaagga gaagtaccaa tattgaatac caaagaacat ccgtatttga
      901 gcaatattat aaatgctgca aaaatagaaa atgagcgtat aatcgg
//
LOCUS       DAZGXS010000401          946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGXS010000401 DAZGXS010000000
VERSION     DAZGXS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..273)
                     /locus_tag="ACHKG5_005177"
                     /pseudo
     CDS             complement(1..273)
                     /locus_tag="ACHKG5_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            367..630
                     /locus_tag="ACHKG5_005178"
                     /pseudo
     CDS             367..630
                     /locus_tag="ACHKG5_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            670..790
                     /locus_tag="ACHKG5_005179"
                     /pseudo
     CDS             670..790
                     /locus_tag="ACHKG5_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            787..939
                     /locus_tag="ACHKG5_005180"
                     /pseudo
     CDS             787..939
                     /locus_tag="ACHKG5_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          249 a          230 c          242 g          225 t
ORIGIN      
        1 ttctggttcg ggccactggc gtaaaaatcc tgctccagca gattttctga cacaggctgg
       61 ctgtgtgcgc ggtaacagac cggactgaaa ttccaggaag cttttgccct cagtccgtga
      121 cggcgcaggc tttccgtcac ggtttttact ttaaaatggt aaccctgggc acgcagttca
      181 tccatcaggc gttgtgcatg accgggtaaa agcaacgagg acaacgctgt cgcagtgctg
      241 acggaactgc cgacgtgtac ttatccttat ccgccagtga tataagacgg taattcacca
      301 tttggattgt ccgctccacc caacatgttg tttccttgag gttctcacac cagaaaggac
      361 atcagcatgc tgagcagaga ggacttttac atgataaagc aaatgcgcca gcaaggtgcg
      421 tatattgttg atattgcgac tcagattggt tgctctgaac gaacggtcag acgttacctg
      481 aaatacccag aacctccggc cagaaaaaca cgccacaaaa tggccgaaat ggactatatc
      541 gacatgcgtc tggctgagaa tgtctggaac ggcgaagtta tcctggcgga aatcaaagcg
      601 atgggttata ccggtggccg ttccatgttg cttcgccagt tcaggaagac accggagcag
      661 cgctttacgc aggaacacag tctgttcttc cggttgctga accgtcgata tgaaaaagcg
      721 agcattgtgc tgacgtccaa taaagggttc gccgactggg ggagagatgt tcggtgataa
      781 cgtgctggca aaatattcac ccaacgcatt gagcgtaata atctgacgct acgcacccgc
      841 attaagcggt tgactcgtaa aaccatctgc ttctcacgct cagttgagat ccacgaaaaa
      901 gtgatcggag cgtttatcga aaaacacatg ttctactaat tggatg
//
LOCUS       DAZGXS010000402          945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGXS010000402 DAZGXS010000000
VERSION     DAZGXS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..485)
                     /locus_tag="ACHKG5_005181"
     CDS             complement(<1..485)
                     /locus_tag="ACHKG5_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0871062.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IP"
     gene            462..604
                     /locus_tag="ACHKG5_005182"
                     /pseudo
     CDS             462..604
                     /locus_tag="ACHKG5_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(799..912)
                     /locus_tag="ACHKG5_005183"
     CDS             complement(799..912)
                     /locus_tag="ACHKG5_005183"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0871063.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          247 a          265 c          230 g          203 t
ORIGIN      
        1 gggatatacg ggagcagttt tttggcaata tgtaaaccct ggccgacaca gccgtactgc
       61 cctttgctca ggtcagccct cggatgattc agcgtactca tatcctgcac atcatgcaga
      121 cagtggtcag ccggaataat atcgttatat ctgcaggcag ccccgcccgg cgtcactgta
      181 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtatgaatc cggcagcgga
      241 agcccttcac cgtaagccat ggcattggac tgcccggcca gtacgatgac gtagtaccaa
      301 tccggctcag atgaagggcc gacctgtggc tctccttcaa tagccaccgc ctgcatcagt
      361 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      421 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata atgtttaatc
      481 gacatcgaat ttctcccgtg tagaggaaca gagttaaaaa gccggaagcg gaatcaaatc
      541 acaggatgac catctgccag tggctggtcg taaaaaaagg ccgcgccatg cgcagccgaa
      601 aataaaggga taacgatgat agtttgagaa aaacagaaat aacacttttg tggcaaagca
      661 tggtgccggg tgcctcccgg tgaattcagt atcagcacct gaatccgcga ttaccccata
      721 ttccttcttg ctgattgccc caccgcacag ggggattcac catgcagaag tgtttttaat
      781 aaacagcaaa caaaaaaatc aagcattatg caggctgttt ctttttatca ccggccacag
      841 caataccata atgccgcaga ccagcacccc atccgccagc accgacatga ttctgctggt
      901 gaaatccacc atcaccacca gaaacagcag gagtgcagcc acagt
//
LOCUS       DAZGXS010000403          937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGXS010000403 DAZGXS010000000
VERSION     DAZGXS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..899
                     /locus_tag="ACHKG5_005184"
     CDS             <1..899
                     /locus_tag="ACHKG5_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFP0871064.1"
                     /translation="HLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVNDLTGRPL
                     MLFNSEGKTVWRPGQTSLWGLALSLPADTDYPDPRGERDAEADPGLLYAGQWQDAESG
                     LCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDY
                     IGDGRRVSGHTGFLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGL
                     RNIYLRKNATLYEVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEA
                     EIAHAIKYVEGLREKFMMGLIN"
BASE COUNT          267 a          185 c          269 g          216 t
ORIGIN      
        1 ggcacctggt gtttaacggc tttgagctga taagccagca gtgcagccgg gtacggcagc
       61 cgcacccgtc cgtggccccg cagtgggtga cacgaacgaa tcatgcggtg aacgacctga
      121 cgggccgtcc gctgatgctc tttaacagtg aaggtaaaac cgtctggcgg ccgggacaga
      181 ccagcctgtg ggggctggca ctcagcctgc ccgcagacac agactacccg gacccgcgcg
      241 gggagcggga cgcggaagcc gaccccggcc tgctgtatgc aggacagtgg caggatgcag
      301 aatcggggtt gtgctataac cggttccgat attacgagcc ggaaaccggg atgtacctgg
      361 tgagtgatcc actggggttg cagggagggg agcagactta ccggtatgtg ccgaatcctt
      421 gtgggtgggt tgatccgctg ggattggctg caagttctaa aatcagcagt ttgatggact
      481 atattggcga tggtcgtcgt gttagtgggc atacgggttt cctggatggg gttcgtttat
      541 cacgtagtca aataaacaat attgctaaag aaatggagaa gctaggaatt aaagtaataa
      601 ggaaagcaga taaatatttg ccaccaaatg ctagggcagc ttttgattat ggtcttcgca
      661 atatttatct taggaaaaat gctaccttat atgaggtgta tcatgaagtg attcatgcta
      721 agcaatttgc gaaaattgga cgagaagcat acgaagcact aggacgttta tctagggagg
      781 aacacgttct aaatgaaata ttaaaaagta aaaatttatt caatgaagcg gaaatagctc
      841 atgccataaa atatgttgag ggattgagag aaaaattcat gatgggacta ataaattgat
      901 aaaacataac aaaataacta tcgaaatggc tcttgac
//
LOCUS       DAZGXS010000404          934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGXS010000404 DAZGXS010000000
VERSION     DAZGXS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..102
                     /locus_tag="ACHKG5_005185"
     CDS             <1..102
                     /locus_tag="ACHKG5_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0871065.1"
                     /translation="LLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            180..395
                     /locus_tag="ACHKG5_005186"
     CDS             180..395
                     /locus_tag="ACHKG5_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0871066.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            400..933
                     /locus_tag="ACHKG5_005187"
     CDS             400..933
                     /locus_tag="ACHKG5_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0871067.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          212 a          215 c          304 g          203 t
ORIGIN      
        1 ctgctgggcg caccggcagc tccggagccg gaaggcatcg cgggtgaggg gagtgcttca
       61 gatagcggtg accccacacc ggacagcgac atgatgatgt aagcatgcgt cagggaccat
      121 cggtgtgtgc cggtggtctt ttttattgtt gtgagcttcc ggattgcggg agacggggta
      181 tgtaccagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg gtgggaacgg
      241 gctactggtt cctgcagttg ctggacaggg tttccccgtc tcagtgggcg gcaataggcg
      301 tgctggggag tctgctgttt gggctgctga catatctgac gaacctgtat ttcaagatta
      361 aagaagaccg gcgtaaggcg gcacggggag agtaagctga tgagcaggaa actccgctat
      421 ggtttatcgg ctgccgttct ggcgctgatt gccgcaggtg cttctgcgcc tgaaatcctc
      481 gaccagtttc tggatgaaaa ggaaggtaac cacaccacag cataccgtga tggtgcgggt
      541 atctggacca tctgccgtgg agccacccgg gtggatggta agcctgttat tcctggcatg
      601 aagctgtcga aggaaaaatg cgaccgggtt aacgccattg agcgtgataa ggcgctggca
      661 tgggtggaga aaaacatcaa agtgccgctg accgaacccc agaaagcggg gatcgcgtca
      721 ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt
      781 aatgcaggtg atcgaaaagg tgcctgcgaa gctattcgct ggtggattaa ggacggtggc
      841 agagactgcc gtattcgttc aaacaactgt tacggtcagg tatcccgtcg tgaccaggag
      901 agcgcgctgg cgtgctgggg aatcgacaga taag
//
LOCUS       DAZGXS010000405          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGXS010000405 DAZGXS010000000
VERSION     DAZGXS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            269..>928
                     /gene="tccP"
                     /locus_tag="ACHKG5_005188"
                     /gene_synonym="espF(U)"
     CDS             269..>928
                     /gene="tccP"
                     /locus_tag="ACHKG5_005188"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFP0871068.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAPNWPAPPPPVQNEQS
                     RP"
BASE COUNT          276 a          243 c          173 g          236 t
ORIGIN      
        1 cttaagagta gatttttggc attttgggaa gatcagcggg acataagaga catacaacac
       61 aggaggaatg gctgtttttt aatagtgctt tgaattccgc atgaaacctt tcttagcagt
      121 cgaaaagcaa aacatacgtt ttctttttaa caataattga tgagtattgc atcgagtgtc
      181 atttatacat cgccgcattt atttgccact ataaaaaggc tgattacata tctatcggag
      241 aactaaaatt acttataagg taatctgcat gattaacaat gtttcttcac tttttccaac
      301 cgtcaaccgc aatattacag ctgtatataa aaaaagcagc ttctctgtat caccacagaa
      361 aatcacatta aatcctgtaa aaatcagctc acctttttca ccaagcagta gctccatcag
      421 cgcaacaact ctctttcgag ccccaaacgc ccattcggca tcatttcatc gacagtctac
      481 tgctgaaagt tcgttacatc aacaacttcc taatgtgagg cagcgcctga tacaacatct
      541 tgcagagcat ggcattaaac ctgcccggag tatggctgaa catattcctc cggcacctaa
      601 ctggcctgcg ccaccaccgc cagtacaaaa tgaacaatca agacctctgc ctgatgtggc
      661 tcagcgtctg gtgcagcatc ttgcagagca tggcattcaa ccagcccgga atatggctga
      721 acatattcct ccggcaccta actggcctgc gccaccactg ccagtacaaa atgaacaatc
      781 aagacctctg cctgatgtgg ctcagcgtct ggtgcagcat cttgcagagc atggcattca
      841 accagcccgg agtatggctg aacatattcc tccggcacct aactggcctg cgccaccacc
      901 gccagtacaa aatgaacaat caagacct
//
LOCUS       DAZGXS010000406          920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGXS010000406 DAZGXS010000000
VERSION     DAZGXS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..914
                     /locus_tag="ACHKG5_005189"
     CDS             <1..914
                     /locus_tag="ACHKG5_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0871069.1"
                     /translation="HLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPL
                     MLFNSEGKTVWRPGQTSLWGLALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESG
                     LCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWG
                     SEQSNISDVLNSLGNRALKYANGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAI
                     KNGERVPATTNGPFKVNGKWTSGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDI
                     TLNKTKPSAVDIYSDVFGGIDINDFRL"
BASE COUNT          258 a          195 c          258 g          209 t
ORIGIN      
        1 ggcacctggt gtttaacggc tttgagctga taagccagca gttcagccgg gtacgacagc
       61 cgcatccgtc cgtggccccg cagtgggtga cgcgaacgaa tcatgcggtg agcgacctga
      121 cgggccgccc gctgatgctc tttaacagtg aaggtaaaac cgtctggcgg ccggggcaga
      181 ccagcctgtg ggggctggca ctcagcctgc ccgcagacac agactacccg gacccgcgcg
      241 gggagcggga cccggaagcg gaccccggcc tgctgtatgc gggacagtgg caggatgcag
      301 aatcggggct gtgctataac cggttccggt actacgagcc ggaaaccggg atgtacctgg
      361 tgagtgatcc gctggggttg cagggagggg agcagactta tcggtatgtg ccgaatcctt
      421 gtgggtatat cgatcctttg gggctggcta tatgtcagtt agcccgctgg acgaaatggg
      481 ggagtgagca aagcaacata tctgatgttt tgaactcatt agggaataga gcacttaaat
      541 atgctaatgg tgattggata aaatcagagg ctgcattcaa taaatacata aacatgataa
      601 ataaaagact agaattaaca ggtagtaaat ttagagttga gattcaacca gccataaaaa
      661 atggagagcg agttcctgcg acaacgaatg gaccatttaa agtaaatggt aagtggacat
      721 ccggcactca ttatacaggt ggttccaaac gtctagatgc cggtattatt gatatcacat
      781 ctcctacaaa ccaatatgga ttacatccag ttattgaagg atttgatata acacttaata
      841 aaacaaaacc atcagcagtg gatatatatt cagatgtgtt tggtgggatt gatattaacg
      901 actttcggtt ataaaacaat
//
LOCUS       DAZGXS010000407          919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGXS010000407 DAZGXS010000000
VERSION     DAZGXS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            197..603
                     /gene="ipbA"
                     /locus_tag="ACHKG5_005190"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             197..603
                     /gene="ipbA"
                     /locus_tag="ACHKG5_005190"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          288 a          184 c          171 g          276 t
ORIGIN      
        1 atatttagtc tattgcgtag aactactatt caaaaaatct tattaatgag ataatgagat
       61 tacatcgata acgttctgat tgcaggcata cttatctggg tgtgcctctc ttttttacaa
      121 ctgtacacgg tggcgtatga cgagaaaata tctcacacag gatgaagtct acaggctgat
      181 ggatgcagcc cagagcatgt cctttcctga aagaaatcgc tgtctaatta tgatggcttt
      241 cattcacggt tttagggcca gtgaacttct tgatttacgt ttatccgata ttgatgcatc
      301 aggaaaacaa cttaatattc ggcggattaa aaatggtttc tcgacaacac atcctcttct
      361 tcctgatgaa tataatttga tcaagttgtg gcttaagcag aggaagttaa ttgaaaacgg
      421 agttgaagga gactggcttt ttctctcccg gaaacgccgc cctatcagca gacaacattt
      481 ttttctatca ttcgtgaggc tggaagacgt gcaggattag ctgtaaaagc acatcctcat
      541 atgttacgtc atgcctgtgg ttttgctctg gctgacaatg gtgtcgatac ccgactactt
      601 caggattatc tgggtcatcg aaatatccag catactgtca gatacacagc cagtaatgct
      661 gctcgattta aaggggtgtg gaaaaaaaag cctcgttaaa acagtacctt ctgattcaaa
      721 atgtcagatt cctacgtttt cctcaattct tccataaaaa aacagcccat tcaattcaat
      781 aaaacacata acatgttgat atttctcgct aaagtaaaat aaccccatga tttgaaatga
      841 ttaaccgcca aggtcatacc agtgattaac tttcactcat gttgttaaat gcaaccaaat
      901 taaaaacaat aaaaatcaa
//
LOCUS       DAZGXS010000408          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGXS010000408 DAZGXS010000000
VERSION     DAZGXS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            280..587
                     /locus_tag="ACHKG5_005191"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHKG5_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(827..>916)
                     /locus_tag="ACHKG5_005192"
     CDS             complement(827..>916)
                     /locus_tag="ACHKG5_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP0871070.1"
                     /translation="PPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          282 a          205 c          177 g          252 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttgagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcgg
//
LOCUS       DAZGXS010000409          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGXS010000409 DAZGXS010000000
VERSION     DAZGXS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            260..335
                     /locus_tag="ACHKG5_005193"
     tRNA            260..335
                     /locus_tag="ACHKG5_005193"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Ile,seq:cat)
BASE COUNT          274 a          159 c          194 g          280 t
ORIGIN      
        1 taatgtaata aacttatatt attagataca aaatcattac aaagtatcat tgaatcgatg
       61 ataactatag attgtgctaa ttattaacat ttgcttgttg actgcgagta tcgactttgc
      121 ggaatggagg tggtgtcggg ttttatgtcg catgtattga cgacccagga tggatgagaa
      181 cgcataaaaa tctgtgattt acatcataag atcatcatgt ttattgcgtg ggttataagt
      241 attgttattg catacttaag gccctttagc tcagtggtta gagcaggcga ctcataatcg
      301 tccggtcgct ggttcaagtc cagcaagggc caccaaatca tcattcatgt atcaggaaga
      361 gttaagggta caagggttac tcgatggcgg tctatatttc acgattaacg ccatttcaag
      421 gaagaagtgc ggtgggtggt ttcgaatgag ggctggtgtt ttggtacgtg gcacgccact
      481 gcaatgtatt tatctatcag tactcattca aaagtatttg gaaaaaatgc ctgctatgcc
      541 aagcgggcaa attgcttaca tgtggaatta agtaattaac gataaagcca gcgtattatc
      601 atttattata cccattcatt taatcaggtt aattactcaa atcactgaac tattctctgt
      661 tcgctcactg atgataaggg agtaccacaa ccaatgtatg tagaacaatg ccgtagagta
      721 aggatgaaat gtctctgcca cgccttaaac atctactact gactagaaca cccgccgcag
      781 gtagatgagt tttgtgagcg ttacaggagc gtggctatgc tcattaatca acgctattta
      841 gatataaatt tctaacactc gaaaaacgat ttttcactaa taaattataa cgggtggttt
      901 ttatcat
//
LOCUS       DAZGXS010000410          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGXS010000410 DAZGXS010000000
VERSION     DAZGXS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..320
                     /locus_tag="ACHKG5_005194"
     CDS             <1..320
                     /locus_tag="ACHKG5_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFP0871071.1"
                     /translation="HHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESING
                     LYKAEVIHRKSWKNRTEVELATLTWVDRYNNRRLLERLGHIPPAEAEKAYYASIGNND
                     LAA"
     gene            439..895
                     /locus_tag="ACHKG5_005195"
                     /pseudo
     CDS             439..895
                     /locus_tag="ACHKG5_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          252 a          209 c          257 g          177 t
ORIGIN      
        1 tccatcacag tgataaaggt tctcagtatg tatcgctggc ctacacgcag cggcttaagg
       61 aagccggatt actggcatca acagggagta ctggcgactc gtatgacaac gcgatggctg
      121 agagcatcaa tggtctttac aaagcggagg taatacaccg taagagctgg aaaaaccgga
      181 cagaagtgga actggccaca ctaacgtggg tggacaggta taacaatcga cgattgctgg
      241 aaaggctggg ccatattcct ccggcagaag cagaaaaagc ttattatgct tccatcggaa
      301 ataatgatct ggcagcctga gttcacagat aaaatactct ccaggaaacc cggggcggtt
      361 cagtctcagt ccggattatg ggggaaaatt tctcaatggt ccggtatcaa caaccagggc
      421 cgggaggatt acaggaaacg agattttgcc ccggacagcc ccgaaataaa attcagtgat
      481 cgcagggaag cccttgaact gatgacgcaa atcgaatcga ccgtgaccag cctgcaccgt
      541 gaggctgaag cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt
      601 tttcgtggca cggccctgta cgccacagaa aacattgccg gtcgcatcaa tgcccgcctg
      661 gaggatgagg gctttaccgt aaaaatcagt tttccggcag tcagccagtt acagacccgg
      721 ctcgcggtaa aaataaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt
      781 cgccgtcggc agagtggcgt atggggaacc gtttgtcgat ggtttggcac cagtgacctg
      841 ggctgggaaa actatgacga ggatgtgagt cgcagcgtga tcaatatcaa caagg
//
LOCUS       DAZGXS010000411          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGXS010000411 DAZGXS010000000
VERSION     DAZGXS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..747
                     /gene="ydeO"
                     /locus_tag="ACHKG5_005196"
     CDS             <1..747
                     /gene="ydeO"
                     /locus_tag="ACHKG5_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0871072.1"
                     /translation="SVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFK
                     EIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFS
                     ELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISES
                     LLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHF
                     GNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          278 a          174 c          171 g          272 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttta aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacgat gcctgttcct gcgaagtttt tacgtgcttc gaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tcggggaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccaaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaatacgggc
      721 aacacgatga atgctttagc tatttgatta tttgctaacg agtagtcaac cacgcaccct
      781 acgtaaaaat taaaaatggg gcagtcattc cctgccccgc gttgttttta ggcgatatat
      841 ttattgaaat aaataagtga cattcatcac atatttatgc atttgcataa cttgt
//
LOCUS       DAZGXS010000412          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGXS010000412 DAZGXS010000000
VERSION     DAZGXS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            89..817
                     /locus_tag="ACHKG5_005197"
                     /pseudo
     CDS             89..817
                     /locus_tag="ACHKG5_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
BASE COUNT          265 a          189 c          199 g          229 t
ORIGIN      
        1 aaagccagaa ctcttggttc caaagctggc aacaactaac cagttaaaac atacgcatca
       61 aaatcagata aacatttgag gtattgttat gacaaataaa cacccatcat tgttttctcc
      121 ttttatgctg acagaaaaaa taaagctacg taatcgtatc gttatggctc ctatgacgac
      181 ctggtcagcg aatcctgatg gaaccatctc tgagcaagaa ctcgaatttt ataaacgtcg
      241 ctcacaaaat gttggtttag taataacagg atgtacctat gtcacgccaa gcggcattgg
      301 gtttactcat gaatttgccg cgtatgacga ccgtttcatt aacagccttg aaaagttagc
      361 agcggcagcc aaaagtggcg gtgctccggc tattttgcag attttccacg ccggtaataa
      421 agccatcccg gagcttgtac caaataacga tgttattagt gccagcgcat caagcgtaaa
      481 atctggtgac tttatgaaga gagtggtaca aagcagggaa atgactgaaa acgaaattca
      541 ggaaaccatt agagcattcg gagatgtcac aaaaagggcg attaaagcag gttttgatgg
      601 cattgaactt catggtgcgc acggattcct tctacagaat tttttctccc ctctatttaa
      661 ccagcgtaat gaccgatggg gaggagatct cgaaggacgt atgcgcttcc cgctggccgt
      721 attacaggaa gtaaaaaatg tagtctatga atatgcgaca aagccctttg ccatcggtta
      781 cagaatatct cctgaagaat ctgcaacagg ggggctgtga accgccccgg gtttcctgga
      841 gagtgtttta tctgtgaact caggctgcca gatcatcgtt tc
//
LOCUS       DAZGXS010000413          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGXS010000413 DAZGXS010000000
VERSION     DAZGXS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>858
                     /locus_tag="ACHKG5_005198"
     CDS             <1..>858
                     /locus_tag="ACHKG5_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP0871073.1"
                     /translation="SVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDL"
BASE COUNT          212 a          214 c          250 g          182 t
ORIGIN      
        1 cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg agctggactt
       61 tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg gcattctctg
      121 gttttcgtca tccggtgaag agattgaacc gcctgacagt gtgacctttc acatctggac
      181 ggcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga agacgaaagg
      241 ggatacggga aaacgtaaaa ccttcgtgaa caccacgctc ggtgagacgt gggaagcgaa
      301 aatcggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagtatt attcagcgcc
      361 cgttcctgat cgtgtggctt acctgaccgc cggtatcgac tcccagctgg accgctacga
      421 aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgacc ggcagattat
      481 tatgggccgc cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac
      541 ctatacccgc cggaatggtg cagaaatgtc ggtatcccgt atctgctggg atactggcgg
      601 gattgacccg accattgtgt atgaacgctc gaaaaaacat gggctgttcc gggtgatccc
      661 cattaaaggg gcatccgtct acggaaagcc ggtggccagc atgccacgta agcgaaacaa
      721 aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga tttataaccg
      781 cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact tcccgaataa
      841 cccggatatt tttgatct
//
LOCUS       DAZGXS010000414          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGXS010000414 DAZGXS010000000
VERSION     DAZGXS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            162..725
                     /locus_tag="ACHKG5_005199"
     CDS             162..725
                     /locus_tag="ACHKG5_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0871074.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            722..>849
                     /locus_tag="ACHKG5_005200"
     CDS             722..>849
                     /locus_tag="ACHKG5_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871075.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDQAAHALAIFKQLR"
BASE COUNT          221 a          202 c          255 g          171 t
ORIGIN      
        1 ggtttttatc tttattattc agtttgttgt gcggaaaaaa tgttaacagg ctttttcagc
       61 aaatgttaac caggcagcag ttaacatttg cggcatgaga cgccgggaaa aatgggctga
      121 accatacccg gctgagtgcg ttctggaccc gggaggaggc tgtgctgaca acgcaaaaac
      181 gaaaatttgc gctggcgctc atgtccggga aaaacaaaac agcgtcagcc cttgccgccg
      241 gttattcggc gaagacagcc agggttaaag gctcgcagct ggcaaaagat ccggaggtgc
      301 ttgcgtttat agcccgtaaa cagtgcgaga cggtggaggt ggatgaggtt cctgtttacc
      361 ggcagaaaaa atcagagcag gaggataaac cccgtcgccg tgaggcggct gcaataccac
      421 agccggacga aaacaatccg gagatgccac cgcccgtggt gatatctcct ggtattgagt
      481 atatggagga cggtcttccc gatccggtga aagcgatggg gcgtcttctg gtggagaaca
      541 ttaataccga ccccaggctg gcgctggatg cggcttataa gctggcgcag ttcacgcacc
      601 acaaaaaagg ggatgccggt aaaaaatcgg caaaaggtga cgcggcgaaa aaagcggcta
      661 accgttttgc ggtgccacca ccaccccgcc tggtggtgaa taatgataat gagggcaacg
      721 gatgatacct gtgtggagca cggcctgccc ggactgggca gagcgcctga aaaaggggct
      781 gtcgattatt ccggatccga tttatccgga ccaggccgca catgccctgg cgatttttaa
      841 acaactgcg
//
LOCUS       DAZGXS010000415          847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGXS010000415 DAZGXS010000000
VERSION     DAZGXS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(213..787)
                     /locus_tag="ACHKG5_005201"
                     /pseudo
     CDS             complement(213..787)
                     /locus_tag="ACHKG5_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          274 a          192 c          130 g          251 t
ORIGIN      
        1 taatctcaaa aaaaacaaac ttactatttg acttggatta tgtttttaat ccaatatttt
       61 ccgacaacat ctccacagaa aaatgcattt acataaagac gccttcaagt catctgaaac
      121 ctgccaacca cagacacctt cttatattct ggatgtctgc ggcgtttttt tacaaattac
      181 tgacagccaa tactctctca gagaaaaata ttttaactat cttttataat gaagtttccc
      241 ttgataggat cataaacaca tttatcagga cttataatca ttgatgccgt tattggttct
      301 cgggtcagtg gatgatatga gccttcacta gctaaacgag aaaatgcatc aaaatcaaat
      361 aagcagcata ctgccgaact atctgaattt ttgacaaaca ctccttcttc gggtctctct
      421 agtgtaattg gacactgaat agactctggc gggcactgga aatttcctga actaacagga
      481 aacttacatt gagatatttt gtcctgaata ctctcttgcg attgtgcttc acctgagtcc
      541 gaaagcatac tgagcatcct atcgcgaaga gcttcagggc cgttattaaa cccataaagg
      601 aataactcag taagcaatcc cccactactg ccgctggata gaaaacgccc atcggtttca
      661 ttaaatacaa tgctgacagt ctcattacct aatgtaaact cccctcgacc gctagatgct
      721 acttgagctc gaaggacatt caatccatta aaaatgagct tgatctaatc tccgaattta
      781 acggcattat aaaactccct attttaattt aaactccaga cttaaatagc tttaacaaac
      841 atctgca
//
LOCUS       DAZGXS010000416          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGXS010000416 DAZGXS010000000
VERSION     DAZGXS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            86..296
                     /locus_tag="ACHKG5_005202"
                     /pseudo
     CDS             86..296
                     /locus_tag="ACHKG5_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            254..583
                     /locus_tag="ACHKG5_005203"
                     /pseudo
     CDS             254..583
                     /locus_tag="ACHKG5_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(721..>846)
                     /locus_tag="ACHKG5_005204"
     CDS             complement(721..>846)
                     /locus_tag="ACHKG5_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0871076.1"
                     /translation="ICYEISQRKYIVTNPADYDKYSDMVKKHVIDGEELIIKYFM"
BASE COUNT          238 a          183 c          184 g          241 t
ORIGIN      
        1 ttaataaaaa taagaacgaa gattaagtta tccagaggtt actgttgcgt ctgtggtcgt
       61 aacaacatcc gacgtggtga tgtgtcaaat gagacattct gatgatcaga gggtttatcg
      121 cgtatacgga tcgtgggtgg cagaaaactt tcagaatcag gtttccattt taaaccagaa
      181 attgagtgag tttgccccat ccataacccc acgcggtgag atcggatgtc attaaacaag
      241 ccaggtcaac aggtcagtat gggaaggcga acaactcgca ggagtgggtg cgctaaagtt
      301 gctggatgat aaacatggcg aactgaaatc aatgcgtacc gcgccaaatt atttacgtcg
      361 gggtgtcgcc agtctgattt tacgccacat tttgcaggtc gcccatgaca gatgccttca
      421 tcgcctgagc ttagaaacgg gtacacaggc tggatttacg gcctgccatc aactttattt
      481 gaagcatggt ttcgttgatt gcgaaccgtt tgctgattat caatttgatc cacacagtcg
      541 atttttgtca ttgacgctat gcgaagataa tgaattgcca tgagccagac gcagcgcatt
      601 cttgcattcg acgtgctgcg cctttattta tcaccagcag gaaacgcctt gtccatagac
      661 gctccttcca catgcgtcac aagaaacctc tattccagtg acacaattac gcctaattaa
      721 ttacataaaa tatttaatta tgagttcctc accatctatt acatgctttt taaccatatc
      781 ggaatattta tcataatcgg ctggattcgt aacaatatat tttcgctgcg atatttcata
      841 gcagat
//
LOCUS       DAZGXS010000417          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGXS010000417 DAZGXS010000000
VERSION     DAZGXS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..845
                     /locus_tag="ACHKG5_005205"
                     /pseudo
     CDS             1..845
                     /locus_tag="ACHKG5_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
     misc_feature    267..383
                     /locus_tag="ACHKG5_005205"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          185 a          202 c          267 g          191 t
ORIGIN      
        1 cgtttttccc ccgaagtccg tcagcgggcg attcgtatgg ttctggaaag tcaggatgaa
       61 tatgactcac agtgggcggc aatttgttcc attgccccaa agattggctg tacgccggag
      121 actctgcgtg tctgggttcg ccagcatgag cgggataccg ggggcggtga tggtgggctc
      181 accagcgctg aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc
      241 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
      301 tggaaaaaat gatgccactg ctggataagc tgcgtgagca gtacggggtc ggaccggtat
      361 gcagcgaact gcatattgcc ccgtcaacgt attaccattg tcagcaacag cgacatcatc
      421 cggataaacg cagtgcccgt gcgcagcacg acgactggct gaagagagag atacagcgcg
      481 tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg
      541 aaggaatcag ggtggccaga tgtacagtgg cacgtctcat ggcggttatg ggacttgccg
      601 gtgttctccg gggtaaaaag gtccgtacga ccatcagccg gaaagccgtt gccgcaggcg
      661 accgcgtaaa ccgtcagttc gtggcagaac gacctgacca gctgtgggtg gctgatttta
      721 cttacgtcag cacatggcag ggcttcgtct atgtggcgtt tatcattgat gtgtttgccg
      781 gatacatcgt ggggtggcgg gtctcatcgt ctatggaaac gacattcgtg ctggatgcgc
      841 tggag
//
LOCUS       DAZGXS010000418          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGXS010000418 DAZGXS010000000
VERSION     DAZGXS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(128..>845)
                     /locus_tag="ACHKG5_005206"
     CDS             complement(128..>845)
                     /locus_tag="ACHKG5_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0871077.1"
                     /translation="EHSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKS
                     GDIMDRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKF
                     NKKNRGFEGLAWKADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVND
                     ISGLAFNNESLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLP
                     DESIYVASEPDILAKFVPNK"
BASE COUNT          239 a          145 c          167 g          294 t
ORIGIN      
        1 tggttgataa taactgatat ccttttcaac tattaatgtg tttatttagc gtgaaattat
       61 actcgtattt ttataacttt atgagattgc caggtttggt cgataaataa taaggccagt
      121 gacagactta cttattaggg acaaatttcg cgaggatatc aggttcactg gcaacataaa
      181 tgctttcatc aggcagagtt acaattcctt caggttgggg caaatcactg gtcagagaat
      241 gatttccttt tgtcaggtca agcatctcaa tgaaagacat ctctgtcagg ttaaacttga
      301 gtaatttacg cgattcatcg gacagaatca tcagactttc attattaaag gccaggccgg
      361 atatatcgtt aacacgtata tcatttagtg cctctggaat agttgcttgc tttacactca
      421 gcaggtctgg agaaagttga tatataaata ttttacttgg gcgtctttct tttgcgacaa
      481 acagcatccg atcatctgct ttccatgcca gcccttcaaa ccctctattc tttttattga
      541 atttttctaa ctgaatttta tttcgaaagg agatatgcat atcttttgta actgtaaaaa
      601 agaatagttc agaagtcttt tcgtctactg cagcaaaaat gttcccttgt atgtgttcaa
      661 ttgtttcggc atcagagata aaaggcagtg gggctctgtc catgatgtcg ccacttttgg
      721 ttattttaag tagctgtgca gggctattct gggtggcata caggaaatca tcctctttat
      781 tataagtaag agatgataag ttcaccgtat ttggtaattg ttttataaat gaaagtgagt
      841 gttct
//
LOCUS       DAZGXS010000419          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGXS010000419 DAZGXS010000000
VERSION     DAZGXS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..453
                     /locus_tag="ACHKG5_005207"
     CDS             <1..453
                     /locus_tag="ACHKG5_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0871078.1"
                     /translation="NLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEAD
                     PEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGY
                     NGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            459..>841
                     /locus_tag="ACHKG5_005208"
     CDS             459..>841
                     /locus_tag="ACHKG5_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0871079.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDA"
BASE COUNT          160 a          206 c          299 g          176 t
ORIGIN      
        1 aatctgtttg gtctggtcac cgggatggcg gaggacctgc agagcctggt gggggccacg
       61 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcaggcaat
      121 cctgaggcag accctgagca ggagctgacg gaccggtggg tggtggagca gatgtcatcg
      181 ctgacggcca tgacggcctc gtttgtgctg gcgacaccga cggagacgga cggggcgctg
      241 tttcccggtc gcatcatgct ggcgaacacc tgtatgtggg attaccgggg cgatgaatgc
      301 gggtataacg gtccggcagt ggcggatgag ttcgacaacc ccaccacgga tatccggaag
      361 gacagatgca gtaaatgcat gcgcgggtgt gagatgcgcg gcatggtggc taattttggc
      421 ggtttccttt ccattaacaa actttcgcag taaatctaat gacacagaca gaatcagcga
      481 ttctggcaca cacccggcgg tgtgcgcctg cggagtcgtg cggcttcgtg gtgagaacgc
      541 cggaggggga gcggtatatc ccttgtgtga atatctccgc agagccggag gcgtattttc
      601 gtattgcgcc ggaagactgg ctgcaggcag agatgcaggg ggagattgtg gcactggtcc
      661 acagccaccc cggtggtctg ccctggctga gcgaggcgga ccggcggctg cagataaaaa
      721 gtgcactgtc ctggtggctg gtctgccggg gggaaattca taaattccgc tgtgtgccac
      781 atctgacagg acggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg
      841 c
//
LOCUS       DAZGXS010000420          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGXS010000420 DAZGXS010000000
VERSION     DAZGXS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            85..732
                     /gene="nleG"
                     /locus_tag="ACHKG5_005209"
     CDS             85..732
                     /gene="nleG"
                     /locus_tag="ACHKG5_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP0871080.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
BASE COUNT          253 a          170 c          152 g          252 t
ORIGIN      
        1 tttgtttgaa tcctatcaca tatttagtat cttgactcct gtcaccaata tggttcaata
       61 acatagacta aagagggaga aaatatgcct gtcatattaa acttttcgag tgaaagagta
      121 ttgtctgaaa gtgaactgga ggctttacgt catgtcggaa gagttagtca gagtgaacag
      181 cttgttgtga gaggccgcac tatgagactt catcatattt catttatgga cagttttagc
      241 gtggaaccgg tttccggcgg actgttggat cgccttagtg caagagggca tcgccttctc
      301 gcagaaaatc tggagataca gcttaacaga ggccatactt ttttgcaggc attccgttta
      361 tatatggaac agagccgtgc aaccccatgt acgcgccaaa acgtcagtag tgcaatacaa
      421 aacaaaatta actctcatgc atttactgtt agtcatcaag acttttcctg tcatgaacaa
      481 cacctgaatt gcccgataac attatgcatt cctgaaactg gtgtttttgt cagaaatgca
      541 aaaaactcag aaatatgttc attatatgac cacaatgcac tcactgaact tatccgacgc
      601 aacgctccac accctctcag ccgtgaaccc tttgttccgg aaatgattgt cagcaaagat
      661 gaatgccatt ttaatctaat agaacaatat ttttgcatat tagccacaca aaatatatgc
      721 actagaattt gactgtatgg ttcgatttaa atgttgcaaa attaatttta ctagttattg
      781 ccatctattt catactccct aattcttgat acaatctgtc tgtcaaa
//
LOCUS       DAZGXS010000421          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGXS010000421 DAZGXS010000000
VERSION     DAZGXS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>826
                     /locus_tag="ACHKG5_005210"
     CDS             <1..>826
                     /locus_tag="ACHKG5_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0871081.1"
                     /translation="AEWLKVNSDYYLDTITYEDLGLSAFKGKHAQSGAFSEFLDAIEH
                     GYILPGTTLLVESLDRLSREKVGEAIERLKLILNHGIDVITLCDNTVYNIDSLNEPYS
                     LIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIMTASCPRWLSLDDKRTAFV
                     PDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGKESAWGPSVIEKLLANKAL
                     IGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLAPFGISNSSKNPMLINLL"
BASE COUNT          280 a          125 c          184 g          237 t
ORIGIN      
        1 gctgaatggt taaaggtaaa tagtgattat tatcttgata ccataacata tgaagattta
       61 ggattaagtg cattcaaagg aaagcatgca caatcaggag ctttttcgga atttttagat
      121 gctatagagc atggttatat attgccagga actacattgt tagttgaaag tctggacaga
      181 ctttcaagag aaaaagtcgg tgaagcgatt gaacgtctga aattgatttt gaatcacggt
      241 attgatgtta taactctttg cgacaataca gtctataata ttgactcttt gaatgagcca
      301 tattcattaa taaaagccat acttatagca caaagggcaa atgaagaaag cgagataaag
      361 tcaagtcggg ttaaattatc atggaagaaa aaacggcagg atgcactgga atcaggtacg
      421 attatgacgg cgtcttgtcc gagatggctc tccttagatg acaaaagaac ggcttttgtt
      481 ccagaccccg acagggtgaa aactattgag ctaattttta aactcaggat ggaaaggcgc
      541 tcattgaatg caatagccaa gtatttaaat gatcatgctg taaagaattt ctcaggaaaa
      601 gaaagtgcat gggggccttc tgtaattgaa aaattattag cgaataaagc tctgataggt
      661 atatgcgtac cttcatatcg tgcaagaggg aaagggataa gtgaaatcgc tggctattat
      721 cccagagtca tatcagatga tttgttttac gctgtacagg aaattcggtt ggcacctttt
      781 ggtattagca atagtagcaa gaatcctatg ctaataaatc tacttc
//
LOCUS       DAZGXS010000422          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGXS010000422 DAZGXS010000000
VERSION     DAZGXS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>826)
                     /gene="espY3"
                     /locus_tag="ACHKG5_005211"
     CDS             complement(<1..>826)
                     /gene="espY3"
                     /locus_tag="ACHKG5_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFP0871082.1"
                     /translation="DLDSYLKLDFDRLSPKQKQTTLCCFWNKIASSLPEPYNSTIKHN
                     IIFYKDGENLMIRGTISIVNEVVKTYSLPIEKDDNGYYDFSGLYLAHSNISGKDPNKD
                     PDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNANCTNANFNNCRFKKCDLTNMNCTGAI
                     LDNAMIYGKEKEPEMQYPEADQIIQRITYQKSDGNETKGMILTNCSCVKTTFNWADLS
                     ESDCQNVDFSEANLSNTILPDIVRMKGTKLYRTDLFNPILKTEAESTEEKDISPLAK"
BASE COUNT          223 a          147 c          150 g          306 t
ORIGIN      
        1 ttttgctaat gggctaatgt ctttttcttc tgttgattct gcttctgttt ttaatatcgg
       61 attaaaaagg tcggtacggt aaagtttcgt gcctttcatc ctgacgatgt caggaagaat
      121 agtgtttgaa agattagcct cgctaaagtc cacattttgg caatcacttt ctgataaatc
      181 tgcccaattg aaagtggttt tcacacagga gcagttagtt agaatcatcc ctttcgtttc
      241 attcccatct gatttctgat aagttatcct ttgtattatt tgatctgctt ctggatattg
      301 catctcaggt tctttttctt taccatatat catcgcatta tcaagaattg cgccagtgca
      361 attcatattt gtcaggtcgc atttcttaaa tctacaattg ttaaaattcg catttgtaca
      421 attcgcattt gtaaacttga cgccataaaa ataagtatgt tcgaagttga catttgagca
      481 gttacagttt cccatatcta taccgaaatc tatatcggga tctttattgg gatctttacc
      541 ggaaatattg ctgtgtgcta gatatagacc gctgaaatcg taatatccat tgtcatcctt
      601 ttctattggg agtgaataag tctttactac ctcgttaaca atagagatag ttccacgtat
      661 catcagattt tctccgtctt tataaaaaat aatgttatgt ttgatggtac tgttgtatgg
      721 ctctggcaat gaggatgcaa ttttattcca gaaacaacaa agcgtggtct gtttttgttt
      781 gggcgagagg cgatcaaaat ccagctttaa atatgagtct aaatct
//
LOCUS       DAZGXS010000423          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGXS010000423 DAZGXS010000000
VERSION     DAZGXS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            191..760
                     /locus_tag="ACHKG5_005212"
     CDS             191..760
                     /locus_tag="ACHKG5_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP0871083.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            760..>825
                     /locus_tag="ACHKG5_005213"
     CDS             760..>825
                     /locus_tag="ACHKG5_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871084.1"
                     /translation="MGWLMISDKLITLVKSLCVLVG"
BASE COUNT          236 a          173 c          210 g          206 t
ORIGIN      
        1 aataaagtca aaacagttgt ttaacactca ggcaccgtaa tgatgccttt gtcatttctg
       61 cgcatctcac gcgcatctca caacacagaa cctttcagga tgacccttga ggataccggt
      121 ttggctgtcg gtgcctttct gtgggctgga ttcctgtgag acaaggttca tcactaaaag
      181 gaaataaccg atgaatatga tggccgtgcc gtttcacggc aactctcttt atgtagttaa
      241 ccataatggc gaaccatacg ttcccatgaa acctgtcgtt gcggggatgg ggctggcctg
      301 gcaatcacag ttggctaagt taagacagcg ttttgcgtca actataacgg aaatcgttat
      361 ggttgctgag gatgggaaac aacgcaatat ggtgtccatg ccacttcgaa aacttgccgg
      421 ctggctacaa accattaatc ccaacaaagt aaaacccgaa atccgcgata aggtcatccg
      481 gtatcaggaa gagtgcgacg atgttcttta cgagtactgg acgaagggtt ttgtcgttaa
      541 tccccgtaaa atgagcgtga tggaagaact caaccaggct tgtgctgaca tgaaacggga
      601 taaaaacatt gccagtgtgt ttgctaccgg gctgaatgag tggaaacagg ttaaagccgc
      661 gcatgtatca aaaatccgta cgctggtaaa tgaagcgaat atgctgattg attttgtcct
      721 ggctgataca ggcaaaggga aaataacaaa ggcggattga tggggtggct aatgatatca
      781 gataaactca taacgctggt gaagagcctc tgtgtacttg tcggc
//
LOCUS       DAZGXS010000424          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGXS010000424 DAZGXS010000000
VERSION     DAZGXS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(347..>820)
                     /locus_tag="ACHKG5_005214"
     CDS             complement(347..>820)
                     /locus_tag="ACHKG5_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871085.1"
                     /translation="LVCFNDDDCAQVRELLKQSNGNITDDKIDEITACLSNPQGITCF
                     YYSPLQGYQSFLKNTSQDVSELLSKSLNGTISSTSGLKKSGNAFFGTGKYLKEIDFTI
                     NGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL"
BASE COUNT          272 a          166 c          111 g          271 t
ORIGIN      
        1 ctgattttga aactggctat tatcattctc tgtttccgtt ggatatgaat aactcgtcac
       61 actaaccata gtaacctcgc aatatgacac tttgcatatt ttgacattgt tagaagtatc
      121 aatggctagc atgataaata tctcaatgct aaccgctata tttcatttat ttcaataata
      181 agactatagt gccgttaatt tttatcgatt tccccaatgc aattgcatca ctaatactac
      241 ttattcacta tcaaaaatcg ctcatttttt caaaaagact gtttacacac agatggttaa
      301 caatacgtgt tcaccttaat gataaacgcg ctttatcaag aaaaaatcat aaatcagcag
      361 ttgttgcggc accggaaaat gtaaaatcga ttttatcgct tttggatttt tcttcgtcga
      421 cactaccggt agtaaaataa caagagcact gaagcttcac ttcatttcca acattattaa
      481 ccgccatcaa atacatcttc tgactaaacc catttatagt aaaatcaatt tctttaagat
      541 acttcccagt cccaaaaaag gcatttcctg acttttttaa acccgatgtt gatgaaatgg
      601 taccattcaa actctttgag agtaactcgc taacatcttg agatgtattt tttaaaaaac
      661 tctgatagcc ttgcaaagga gaataataaa aacatgtaat cccctgaggg ttggataagc
      721 aagcggttat ttcatcaatc ttatcatccg taatattacc gtttgattgt tttaataatt
      781 cccttacctg agcacaatca tcatcattaa agcaaaccag
//
LOCUS       DAZGXS010000425          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGXS010000425 DAZGXS010000000
VERSION     DAZGXS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            22..522
                     /locus_tag="ACHKG5_005215"
     CDS             22..522
                     /locus_tag="ACHKG5_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0871086.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            552..818
                     /locus_tag="ACHKG5_005216"
     CDS             552..818
                     /locus_tag="ACHKG5_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP0871087.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
BASE COUNT          285 a          144 c          140 g          251 t
ORIGIN      
        1 ttttagagta caggttggca tatgcaagtc ttttccagtg atgtctactt cactgtaggc
       61 actaatgcat tattagcatc ccaaaaggaa tattacagcg atttagttgc gttggttgat
      121 cttggacatt cgttcgttgt tattgatgag catcaacatc gaaatctcaa accaaataca
      181 gaacctgtaa acatcctttt gagtaacaat ttcatctgga tcaataagaa tattacatta
      241 tctgatttaa cgcatttcct ggtttcaaat cttcatacac aaaatgtgta ttccacacaa
      301 gaaccactta cccatgatga gatagatatc ctgcggttat gtgtttcata ttcattaaag
      361 caaattgcca ttattaaagg aattgattat aaaacagtat catatcacaa aataagagcc
      421 ctcaatcaac ttaacataaa aggtactgtt gagttgttta tagcactctg tgaatgggat
      481 aaacattatt ttaaactcca atcttgcgta agagaaagtt gatatgtcat tagtaatgat
      541 ataatagtaa aatcataata accatcgcgc tgatatcgtt gctctctgca tgcaccagag
      601 aaaattcatt aaaggagaat caaaataaaa gaagtggcga atcccaagcg atagtagatg
      661 caagacagtt ctttagcgaa cacccggaat atatatcatc agcagaactg gaggaaaagc
      721 tatatcgcga atttgcacag gttactacag ttccagctta taaagagatg agtatgtatc
      781 aattgcttgt catttcgcaa gatcgtttaa ctcattaaat
//
LOCUS       DAZGXS010000426          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGXS010000426 DAZGXS010000000
VERSION     DAZGXS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(123..698)
                     /locus_tag="ACHKG5_005217"
     CDS             complement(123..698)
                     /locus_tag="ACHKG5_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0871088.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
BASE COUNT          261 a          186 c          149 g          223 t
ORIGIN      
        1 gcaggagata attgagatat acttgggata cttaaggtgg taacaggcat aaaaacctca
       61 ttcagtaatt catatcagtt tgcaggatgt ttcattaaat aaaataaagg gtaatgttca
      121 tttcaattac cctttataac gaagtttcct ctggtatcgt cataaatgca ttcttcatgt
      181 tttacaatta ttgatgccgt tattggttcc cgggtcagtg ggtggggtaa gccttcacca
      241 accaaacgag aaaatgcagc ggcatcaaat aaagtacata catctgaacc atctgaattc
      301 ttcacaaaaa tacctttttc aggctgctcc agtgtaattg gacactgaat agcctcaagc
      361 gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt gttctgaata
      421 ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct ctcaccaagg
      481 gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc gccattaccg
      541 ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt ttcaccaccg
      601 actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc caccccaaga
      661 ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc tattttaatt
      721 tgaactccag acttaaatag ctgtaacaaa catccaccgt acatgacaac aaaaaccgga
      781 gccggactcc ggttttgtga agctgtcggg ttacttcat
//
LOCUS       DAZGXS010000427          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGXS010000427 DAZGXS010000000
VERSION     DAZGXS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHKG5_005218"
     CDS             complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHKG5_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0871089.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCW"
     gene            complement(750..>819)
                     /locus_tag="ACHKG5_005219"
     CDS             complement(750..>819)
                     /locus_tag="ACHKG5_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP0871090.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          239 a          155 c          158 g          267 t
ORIGIN      
        1 tgccaacaat ctgaagtaat actggtttta ttaaagtacg cagggggtgt taagatggct
       61 tcttttcggg tgaattttgc acaatactta gaaatcgtct ccttaataca ttccagctct
      121 tttttattga cggctgagca agtacatttt tcgccaaaag gtgcaaaagt gcctacatct
      181 aatgtttgta tgatataatc gtcatgttct cctgtagaaa tatctatgat gaatggactc
      241 atattttccg gttggattcg gatcatgaca gaaggaacat cgccttgata gaaattcaat
      301 gcagtaattg cagataatac tctccgcagt tcattactta tactgtaaat tttattaacg
      361 tcattggtgg agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttcta
      421 ttgtgattgt taaccactgc gtgcattggt gggattgaag ggtgaaaaat atgtgcaatt
      481 gggaagcctt tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattt
      541 tcctcgcagg gttctttcgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac
      601 gaagcaacat tctgtggcct acgccgatca aaagctctgt gaaaatttcc cttgttgtct
      661 ttgtctgacc ttatcgcggc gatcagcaat tattcgtttt gtgatcctca acacaaaatg
      721 ctattcgtcg cctatgaagg aagaagaatt tatggaaaac gattgcattc acaaaatgac
      781 gtcctgtcag aagagaaaaa gttgacgtta atcaattgt
//
LOCUS       DAZGXS010000428          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGXS010000428 DAZGXS010000000
VERSION     DAZGXS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>794
                     /locus_tag="ACHKG5_005220"
     CDS             <1..>794
                     /locus_tag="ACHKG5_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP0871091.1"
                     /translation="NPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLAL
                     RSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWL
                     GWGHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPL
                     MAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNP"
BASE COUNT          157 a          184 c          286 g          167 t
ORIGIN      
        1 aatccgaatc tcggctattc agtggatgag gcctttctgt accgggagta ccgtaaagcc
       61 cgggaagccg gtgaagagac attccggggg ttcatgtcaa aacacgccaa tgtggaaatt
      121 ggtcttgccc tgcgctctga ccgctgggcg ggggctgatt tctgggaaga gcagggccgt
      181 tgtatcagcc tggacgatat cctgcgtcgt gctgatgtgg tgacggtggg gattgacggc
      241 ggagggctgg atgatctgct ggggatgtat gtgattgggc gtgaccggga gacccgcgaa
      301 tggctgggct ggggccatgc ctgggcgcat gaaaccgcgg tggtccgacg gaagagcgag
      361 gcgtcccggt ttcaggatct tgttgcctgt ggagatatga ccattgtccg gcgtgtcggg
      421 gatgacacgg cggaagtggc ggaatatgtg cgtcgcattc atgaggctga gttactggac
      481 catatcggta ttgacccgtc aggggtgggg cagattctgg attcactggc ggaagccggg
      541 atccccgacg gaattgtggt ggggataagc cagggctgga aactgggcgg ggccattaaa
      601 accaccgagc gcaaactggc tgaaggggtg ctggtgcatg gtgaccagcc cctgatggcc
      661 tggtgtgtcg gcaatgcccg ggtggagcct aaaggtaacg ccattcttat caccaaacag
      721 gccagtggac ggggaaaaat tgacccgctg atggcgctgt tcaatgcggt ctccctgatg
      781 tcccttaacc cgga
//
LOCUS       DAZGXS010000429          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGXS010000429 DAZGXS010000000
VERSION     DAZGXS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..126
                     /locus_tag="ACHKG5_005221"
     CDS             <1..126
                     /locus_tag="ACHKG5_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP0871092.1"
                     /translation="ITGWKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            129..578
                     /locus_tag="ACHKG5_005222"
     CDS             129..578
                     /locus_tag="ACHKG5_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFP0871093.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
     gene            575..>793
                     /locus_tag="ACHKG5_005223"
     CDS             575..>793
                     /locus_tag="ACHKG5_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871094.1"
                     /translation="MIIINLRLTLLFILATFTYSVQSDEFDDQTTYTQYRYFSTAIPE
                     DIRRFMFYVDYCFVDLAFDIETDSMTAKN"
BASE COUNT          290 a          129 c          134 g          240 t
ORIGIN      
        1 attactggat ggaaacctat cacaataaaa aatgtagcga tgaattataa tggtggtggc
       61 gatcccaagt atgcaataaa acttcagttc gtttatacat tactcacaaa aaacagagta
      121 ttatgagaat gcgaaaaata acaattattc tactgctgac attctcatgt acaaaaggtc
      181 tggcatttga catttccatg tctaaagaat gtagtggtgt tgatgaactt gaatttattg
      241 attgtgttaa aaactcattt attatgagca ctattacact taaaaaagca gaagcaaata
      301 taaaagagaa aattcaaaaa tggaatagtg atgaggacga agcaataaga aaagaagcag
      361 ttatttcttt aaaaagacta gagtcttcta atagggaatt tatacgttac agatttgctc
      421 aatgctcttt tactctaacc tggggggcca gagtagggcg actggcattc tctcggctat
      481 atccatgcta tactgagtta aacaaaataa gagccatgca actaaacaat gcttacattt
      541 ctgactttca aaatccagat gagatatcca gcaaatgatt attataaatt taagattaac
      601 attactattt atactggcca catttacata ttcagtacaa tcagacgaat tcgatgatca
      661 aaccacttac acgcaatacc gttatttttc cactgctata ccggaagata tacgtagatt
      721 tatgttctac gtagattatt gctttgtaga tttagcattc gatatcgaaa ctgactcaat
      781 gacagctaag aat
//
LOCUS       DAZGXS010000430          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGXS010000430 DAZGXS010000000
VERSION     DAZGXS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>788)
                     /locus_tag="ACHKG5_005224"
     CDS             complement(<1..>788)
                     /locus_tag="ACHKG5_005224"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0871095.1"
                     /translation="CSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWF
                     LFTGILPCGLLLLVNIRYPETWIKGITYRLISMFASLLIIFAIAFFFYKDYASVGRNN
                     SSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
                     RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKA
                     VNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSD"
BASE COUNT          260 a          157 c          121 g          250 t
ORIGIN      
        1 tatctgaatt tgatggctca atattaattg tagggattcg cttacataca cctttacatc
       61 ccccgtcatt ttcaatccag agtaaattta cccccgttct gtacagaata tccagtacat
      121 tttccgaatt taccgctttg cgggaattga actcttttcg cttcatatct gaaaacatgc
      181 atggaacaga tatggctgtg gatgtgccac acgactgcac attatgaaag gatataagtt
      241 cattatattt ctttgtaaaa tcattggtac cacgagaata tccattcaga gcatagtttt
      301 gacttcttgc tgtctccccg acaactaaaa acataattac cggcttttgt ttttcattta
      361 ttgtgcggga tgcatccgtt ccagtctgac gaaactcgcc aggagaaaca aaaaaatccc
      421 ttacatattt aaacccactg tagatataat tagtaggtat tatttcttta tttaatgaag
      481 agttgtttct gccaactgat gcataatcct tataaaagaa aaacgcaatc gcaaatataa
      541 tcaagagaga tgcaaacatt gatattaaac gatatgtaat accctttatc catgtctcag
      601 gataacggat atttactaac aacaatagtc cacatggaag tatacccgta aataaaaacc
      661 acagtacaac agacatattg aaatatgatg tcatttcacc tgcatttgtt tcaaaaatat
      721 tctgcatcat tgttttatca aactgcacac catatttcat ggtggcatat gtaacaatgg
      781 aactacag
//
LOCUS       DAZGXS010000431          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGXS010000431 DAZGXS010000000
VERSION     DAZGXS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            14..>779
                     /locus_tag="ACHKG5_005225"
     CDS             14..>779
                     /locus_tag="ACHKG5_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0871096.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAEVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSH"
BASE COUNT          242 a          174 c          141 g          222 t
ORIGIN      
        1 accggagtag tgtatgacca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
       61 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
      121 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
      181 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttaaatc tgcatcacca
      241 tcttgagaaa aatctcagag gccttgaaca aacaattaat atagtcaata agtccgaact
      301 aaagaaaaac tttattattc acggccctca gctcatttct tgctctaata atagtatgct
      361 gatccgctgc ctacggcaag attcctctgt cgagattgag tgccatgata tcctcatgtc
      421 agcagagaat gctgaagaac tgttggttca ccgcaaagcc gatctggtga tcacccaaat
      481 gccagtgatc agccgttccg tcatttgcat gccattgcat acgattcgca atactctaat
      541 ttgtagtaat aggcatccac gaattactga caatagcact tatgaacaaa ttatggcaga
      601 agagttcacc caattaatat ctaaatcagc agaggttgat gatattcaaa tggaaataga
      661 cgagagattt atgaatcgca aaatttcatt tcgtggttcc tctttgctga caatcattaa
      721 tagcattgca gttacagatt tactgggaat agtcccctat gaactttata actcccacc
//
LOCUS       DAZGXS010000432          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGXS010000432 DAZGXS010000000
VERSION     DAZGXS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>773
                     /locus_tag="ACHKG5_005226"
     CDS             <1..>773
                     /locus_tag="ACHKG5_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871097.1"
                     /translation="DHITIGIDNQEPIKLPASYKDKELVRTIINDNIVEKTHDDINNA
                     EFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIKSPANDENKKVGQTIINENIVENTHDI
                     NNKKMIFSAIKEIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTGLFTWYGKDKKGD
                     SLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARSASAWFEHSKA
                     IIAESFATGSDHQVVNELKGERLRELNEAFNPSNLARGY"
BASE COUNT          302 a          133 c          144 g          194 t
ORIGIN      
        1 agaccatatt actattggta tcgacaacca agaaccaata aaattacctg cgagttataa
       61 agacaaagaa ctcgtccgta ctatcattaa tgacaacata gttgagaaga ctcatgatga
      121 tataaataat gctgaattta ctgatggtgg tattgatgat cgtagtgata ttggcattga
      181 ctccaatgat acagacgata ttgatattga taatcaagaa ccaataaaat cacccgcgaa
      241 cgatgaaaac aaaaaagtcg gccaaaccat catcaatgaa aacatagtag agaatactca
      301 tgatatcaat aacaagaaaa tgatcttcag cgcaataaaa gaaatatatg atgaagatcc
      361 aagttttatc ttcgataaaa tatcacacaa actcagacat acggtaacgg aattcgatga
      421 gagcgggaaa agcgaaccaa cgggcttatt tacctggtac ggtaaagata aaaaaggcga
      481 ctctctcgct attgtaatta aaaataaaaa tggaaatgat tacttatctc tcggttacta
      541 cgatcaggat ggctaccaca ttcaaaaagg atttcgtatt gatggtgata atctcaccca
      601 atattgtagt aaaaaagcca ggagtgcttc tgcgtggttt gaacacagta aagcaatcat
      661 tgcagaatca tttgcaacag gttccgatca tcaagttgta aacgaactca aaggggaaag
      721 actgagagaa cttaacgaag cttttaatcc ttcaaatcta gcaagaggat atg
//
LOCUS       DAZGXS010000433          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGXS010000433 DAZGXS010000000
VERSION     DAZGXS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..93
                     /locus_tag="ACHKG5_005227"
     CDS             <1..93
                     /locus_tag="ACHKG5_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871098.1"
                     /translation="VFKHYPLMKVISAQQAISALKYMVDGEVYF"
     gene            500..598
                     /locus_tag="ACHKG5_005228"
     CDS             500..598
                     /locus_tag="ACHKG5_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871099.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            595..>770
                     /locus_tag="ACHKG5_005229"
     CDS             595..>770
                     /locus_tag="ACHKG5_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871100.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEI"
BASE COUNT          226 a          173 c          161 g          210 t
ORIGIN      
        1 gtttttaaac actatccttt aatgaaagta atatccgccc agcaggcgat aagtgctctc
       61 aaatatatgg ttgatggtga ggtttatttt tgagtaataa tcactttttc aaccgtgcta
      121 tggtatgaaa gtaaagtaac tactatgata ttaactatcg agcaagaata tccccaggca
      181 ctcaacaggg cgcaggtcaa cgtgaatcgg tcatgacacg acaccgattt tgcggaatca
      241 atgttgctta tgaatacaac atcccgctta gtacatgttc aacacatcag caaattttga
      301 actgggtgtg gcacttaact gaaaaaacat ggatgacaca aaatgttact cgtcgcttca
      361 ttgaagtagc ttgtggatat cacaagctag actatcgcca atgatattca atttttgatt
      421 ttttagtctc aaccttccat aataatgtca ccggagcttg aacaactccg gtgacttctg
      481 cgcatttaag gggacttaaa tgcgaccaca atctgaactc cttaccttgt cacagatgca
      541 gaaatgcacc tgcgatgtct tgcatccagc gtttgatctc tgcggaggtg aagcgtgaac
      601 ctcccacaag atggcatcaa attacatcgc ggtaacttca ccgctgtcgg tcagcagatc
      661 cagacttatc tggaagacgg caaatgcttt cgcatggtgc ttaaaccgtg gcgcgagaga
      721 cgcagtcttt cccagaatgc actcagccac atgtggtaca gcgaaatcag
//
LOCUS       DAZGXS010000434          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGXS010000434 DAZGXS010000000
VERSION     DAZGXS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..242
                     /locus_tag="ACHKG5_005230"
                     /pseudo
     CDS             1..242
                     /locus_tag="ACHKG5_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            293..595
                     /gene="bamE"
                     /locus_tag="ACHKG5_005231"
     CDS             293..595
                     /gene="bamE"
                     /locus_tag="ACHKG5_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0871101.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
     gene            complement(671..>765)
                     /locus_tag="ACHKG5_005232"
     CDS             complement(671..>765)
                     /locus_tag="ACHKG5_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871102.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
BASE COUNT          244 a          170 c          155 g          196 t
ORIGIN      
        1 agacaattta taaaacgctg tactttcgta gccgtgaagc gctacaccac ctgaatatac
       61 agcatctgcg acggtcgcat agccttcgcc atggcaggcg tcatacccgc aaaggcgaaa
      121 gaggtacgat taacatagtg aacggaacac caattcacga acgttcccga aatatcgata
      181 acagacgctc tctagggcat tgggagggcg atttagtctc aggtacaaaa aactctcata
      241 tagattcgtt tttctgacct tggcaggttg ttcttcagta ggcaaccagt cattgaaaaa
      301 tgagacgcag gaaagtgtga aaaccaaaat tgttaaaggc aaaactacaa aacaggacgt
      361 gttagcatcg ttcggtgaac ctgacagccg ttctttgatc gatggtgaag aacaatggtc
      421 atacactatg tataacagcc agtccaaagc aacctctttc atccccgttg tgggactgct
      481 tgcaggtggc gcagactcac aaactaaatc tctgacagtt tctttcaaag gcgaaaaagt
      541 cagcacatac atctttaatg ctggaacaag caacgtgaag actggcattt tttagataaa
      601 agacaaaacc agcatcaagg gaaaatttag cccgcagttg atacctacgg gctttttaca
      661 gaaacataaa tcacttttta aagaaagtga ttatcttttt cagcctcccc caaactttac
      721 tgttcatttc tacagtctca gtattttcct ttaaagagga tctta
//
LOCUS       DAZGXS010000435          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGXS010000435 DAZGXS010000000
VERSION     DAZGXS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>756
                     /locus_tag="ACHKG5_005233"
     CDS             <1..>756
                     /locus_tag="ACHKG5_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP0871103.1"
                     /translation="RLSIECHFSNNEREFIKMGYYNGCDVILESDTIQKIKSFAKNIT
                     IQTADGVMANWDDKLNTLSVYSIDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLF
                     YDKYQVDIEANKLTYTVLFPDTELPVQLNYTSIIYGNHGAKYTFLNSGSKTIDIHILD
                     KNSDYDTFDFRNIIFEHYTNEIFISFDNQGGFVISILNNATSEAANINVFRKNMTSLD
                     SSGSLIYLPSGDIYHISDIYKMSRGRKSFKLNVE"
BASE COUNT          280 a           99 c          130 g          247 t
ORIGIN      
        1 cgcctatcta ttgaatgcca ctttagtaat aatgaaagag agtttataaa gatgggctac
       61 tataatggat gtgatgtgat tttagaaagt gacactatac aaaaaataaa atcatttgca
      121 aaaaatataa caatacagac agcggatgga gttatggcaa attgggatga taaactaaat
      181 actctgtctg tttattcaat agatatgatt gcatggaggg ataaaaataa aactgcaaaa
      241 gaaccgttac cagttgatgt aatccagtta actaattgga ggatgcataa cacatgctct
      301 ttattttatg ataaatatca agttgatatt gaagccaata agttaacgta tacagtctta
      361 tttcctgata cggagttacc cgttcagctc aattatacca gtattattta tggtaatcat
      421 ggggcaaaat acaccttcct caattcagga agtaaaacta tagatattca cattcttgat
      481 aaaaatagtg attatgatac ttttgacttc cgtaatatta tttttgaaca ttacaccaat
      541 gagatattta taagttttga taatcaagga ggatttgtta tatcaatatt aaataatgct
      601 acgagtgaag cggcgaacat taatgtattc cgaaaaaaca tgacttcttt agatagtagt
      661 ggttcattga tatatttgcc tagcggtgat atttatcata tttcagatat ctataaaatg
      721 tccagaggta gaaaatcgtt taaattaaat gtagaa
//
LOCUS       DAZGXS010000436          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGXS010000436 DAZGXS010000000
VERSION     DAZGXS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..581
                     /gene="escU"
                     /locus_tag="ACHKG5_005234"
     CDS             <1..581
                     /gene="escU"
                     /locus_tag="ACHKG5_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP0871104.1"
                     /translation="YFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLF
                     SVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKK
                     STVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLAR
                     TLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
     gene            complement(578..>756)
                     /gene="etgA"
                     /locus_tag="ACHKG5_005235"
     CDS             complement(578..>756)
                     /gene="etgA"
                     /locus_tag="ACHKG5_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFP0871105.1"
                     /translation="AGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIY
                     KTYNKLKNNEQIID"
BASE COUNT          234 a          130 c          140 g          252 t
ORIGIN      
        1 tttattttat ggggcattca tatgcaaatg agtttgctaa tttcacagga ctgaacgcat
       61 atcaagctct tgtcgttgtt gccttttttg tttttctttt atggaaaggc gtgctattcg
      121 gatatctact cttttcagta tttgatttct ggttccagaa gcatgaggga ctgaagaaaa
      181 tgaaaatgag taaagatgag gtgaaacgag aagccaagga tactgatggt aaccctgaaa
      241 ttaaagggga gcgccgtcgc cttcattccg agatacaaag tggaagtttg gctaataaca
      301 tcaaaaaatc aaccgttatt gttaaaaacc cgactcacat tgcgatttgc ctatactata
      361 aacttgggga gactccatta cctttagtta ttgaaacagg aaaagatgcc aaagctctac
      421 agatcattaa actggctgaa ctctatgata ttccagtgat tgaagatatt cctttagcaa
      481 gaactctcta taagaatata cataaaggac aatatataac agaagacttt tttgaacctg
      541 tggcacaatt gattcgtatt gcgatagacc ttgattatta atcgataatt tgctcattat
      601 tctttaattt attatatgtt ttatatattt tcattgcgta ttgccttctg agttctactg
      661 catttggtgt attaaagtaa cctgcattat acgcaccaac tgcatcccat gtattccctc
      721 tggattttat gttagaggct aagatataac cagcaa
//
LOCUS       DAZGXS010000437          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGXS010000437 DAZGXS010000000
VERSION     DAZGXS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..105
                     /locus_tag="ACHKG5_005236"
     CDS             <1..105
                     /locus_tag="ACHKG5_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871106.1"
                     /translation="IITQCTFELDCLLTRVSASSNQFRNSNGYLPENS"
     gene            110..448
                     /locus_tag="ACHKG5_005237"
     CDS             110..448
                     /locus_tag="ACHKG5_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871107.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
     gene            435..>747
                     /locus_tag="ACHKG5_005238"
     CDS             435..>747
                     /locus_tag="ACHKG5_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871108.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DF"
BASE COUNT          256 a          107 c          158 g          226 t
ORIGIN      
        1 ataattacgc aatgcacatt tgagctagat tgtttgttaa cgagagtatc tgcgtcatcg
       61 aaccagttta gaaactctaa tggttactta cctgagaata gttaatagca tgaaaaatgt
      121 agaaaatatt atctggcatg atggtgtttt agtagaaacc agaatggaat gtcaccacac
      181 cgagtataat tttatattga cggcggagct ttatgttgat gaatttacag ccggaaggga
      241 acgaaaaagg ttcgagttta caggagtgga acatttatcg caggttatgg atattgctga
      301 acttatagat aaccaaaatg caggtaatat taaagatgcg acaattgttt ataaggcaaa
      361 gaagtataaa atttcacttc atttgtttgg tggtttttta agttttacat ttaaaaccat
      421 gcgagttatt gacaatgaat gtgcgtaatg tcaaattcga caacttgtct ttttatgaag
      481 aaaaaatccg ctggcgaatg cgtcggcaaa tctcgaatgc agaggcaata ttttacgaga
      541 atcaggaaga acacattcgg cgctttttag gcagatatac ctgtcgcaaa ccggttcttg
      601 tttattggga aagtgaacaa agatggacat ctgtaaacgg tctggaagtg atgagtatgt
      661 ttggtgaaca ttgtcatcac atctaccttg atgatatcaa gaaaaaaata caaactgaat
      721 tacctgaaaa taaaacttta gatttca
//
LOCUS       DAZGXS010000438          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGXS010000438 DAZGXS010000000
VERSION     DAZGXS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            65..>742
                     /locus_tag="ACHKG5_005239"
     CDS             65..>742
                     /locus_tag="ACHKG5_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0871109.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDD"
BASE COUNT          244 a          131 c          163 g          204 t
ORIGIN      
        1 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
       61 tcctgtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
      121 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gccgctaaac aaggtgaacc
      181 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
      241 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacatt
      301 cgtgcagggc cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt
      361 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
      421 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatactggtg tgattgtatt
      481 taaaggatca gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg
      541 tgatgtgact gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa
      601 aggaatcgcg gctctaaaag cagctggaat cagtgagttc tcaacaacag aattagaaat
      661 gattgctcaa tctgaagttg agctttcacc agaagattta gaaatctttg aagggcttgt
      721 tgatgccctt gaagatgacg ac
//
LOCUS       DAZGXS010000439          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGXS010000439 DAZGXS010000000
VERSION     DAZGXS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
BASE COUNT          247 a          154 c          142 g          199 t
ORIGIN      
        1 ttaacaaacg actttaataa ttgcatttcc atttagttaa gcgtttctct ttataagata
       61 gatctcattg cataaacaaa taaaacagaa taaaaaccaa gctgaaccgt ttcactatac
      121 tctacttaat tcgagttaca ggaaggcggc aagcgagtga atccccagga gcttacataa
      181 gtaagtgatt ggggtaagcg aacgcagtcg cagcacatgc aacttgaagt atgacgagta
      241 tatatatcta ttagctgttt cccggacata agctacagcc agcactaacc tggaacgggt
      301 gcattataaa aaggaacaag ggacaaatag ctgaacagta accgcgttag tctcttcccg
      361 cataaccgta ccattcgtat taagaagtat tgcccatatc cattcggcga aagaggatct
      421 gccatacatc aagtccgttt tacttggcat cagatcatcg ccgtcattaa ctctgttaaa
      481 gtcggacaaa cgattagcaa gatctgtgat tcaaaagtgc ttagcgctaa ggtgggactg
      541 gttacagatt cattaaccct gttctttgtc gcacctatcg atcggtgtaa tgccacaaca
      601 ggtttatgtt aatcagaaca atgcgcccct ctataaagag aattctttga taaggcaact
      661 tgaaaaggga aagctaactg atatcaatgt accactttag taatcatcac attatataca
      721 ggccaaaata agtttcctga aa
//
LOCUS       DAZGXS010000440          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGXS010000440 DAZGXS010000000
VERSION     DAZGXS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>740
                     /locus_tag="ACHKG5_005240"
     CDS             <1..>740
                     /locus_tag="ACHKG5_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0871110.1"
                     /translation="KDAPSRARKIVQQGTIIYSTVRPYLLNIAIIENEILPEPIASTA
                     FAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFSGITPVPPSLEQ
                     VRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWARISEY
                     FDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIK
                     KQKPLPPISDEEKPFELPEGWEWCLFED"
BASE COUNT          232 a          166 c          157 g          185 t
ORIGIN      
        1 gaaagatgcc ccatcgcgag cgagaaaaat tgttcagcaa gggactatca tatactctac
       61 tgttcgcccg tatttgttaa atatagctat tattgaaaac gaaatactac ctgaaccgat
      121 cgctagtact gccttcgcta ttattcatcc atatacggcg atggatgcta atttcattta
      181 ttattatcta cgttctccgg tatttgtttg ttatgttgaa aattgtcaga caggggttgc
      241 ctatccagca atcaatgaca aacaattttt ttctggaata actccagttc ctccatcctt
      301 ggaacaagtt cgcatcgcaa acaaaatcaa agaattaatg tccctctgcg accaactgga
      361 acagcaatcc ctgaccagtc tggacgcaca tcaacaactg gttgaaaccc tgttgggaac
      421 gcttacagac agccaaaccg ccgaggaact ggctgaaaac tgggcgcgta ttagcgagta
      481 tttcgacact ctatttacca ccgaagccag cgtggatgcg ttaaaacaga ccattctgca
      541 actggccgta atgggcaaac ttgtgccgca ggatccgaat gatgaaccag cctctgaact
      601 gctcaaacga attgcgcagg aaaaagctca actggtgaaa gaaggaaaaa taaaaaaaca
      661 aaaaccgttg ccgccaatta gcgatgagga aaaaccgttt gaacttccgg aagggtggga
      721 gtggtgttta tttgaagata
//
LOCUS       DAZGXS010000441          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGXS010000441 DAZGXS010000000
VERSION     DAZGXS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..537
                     /locus_tag="ACHKG5_005241"
     CDS             <1..537
                     /locus_tag="ACHKG5_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0871111.1"
                     /translation="VMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYRYEWDSPVS
                     VVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHT
                     KAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKAD
                     IYGKHSVNGFTVGVGYRF"
BASE COUNT          207 a           99 c          196 g          232 t
ORIGIN      
        1 gttatgagtg gttcaagact ggctgataat catacgctat cggctggtta tgctcagagt
       61 aaggtacagg actttaagaa tattaaagga gtgaatctgc aatatcgata tgagtgggat
      121 tctccggtca gtgtcgtcgg gagtttttcg tatatgaaag gtgattgggc ggacagtcat
      181 cgcgatgaag ctgacgattt ttacagacat caggctgata ttaaatatta ttcatttctc
      241 gcaggcccag cttatcgtct gaatgactat atcagttttt atggtttggt ggggatatct
      301 catactaaag cgaagggaga ttatgagtgg agaaacagtg tgggtgctga tgagtctgat
      361 ggatatttaa gtgagtctgt atcaaaaaaa tccactgatt ttgcttatgc agctggtgtt
      421 attattaatc cgtggggcaa tatgtctgta aatgttgggt atgagggaac taaagcagat
      481 atatatggaa aacattcggt caatggattt actgttggag ttggttatcg tttttaatca
      541 ttagacggga ctggataatt gcacttgcgt agtgaagttt actaaatgtt tgtattttaa
      601 tgaagtattc gacatattcc cttcagacgg aatatgtcga cggttatggt tttactggag
      661 catgagtatg ttatggcgaa actgataaat atatcagcgt ccactgagga gagaatgacg
      721 ccaggggaga ggag
//
LOCUS       DAZGXS010000442          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGXS010000442 DAZGXS010000000
VERSION     DAZGXS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            46..>730
                     /locus_tag="ACHKG5_005242"
     CDS             46..>730
                     /locus_tag="ACHKG5_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFP0871112.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFV"
BASE COUNT          285 a          134 c          131 g          180 t
ORIGIN      
        1 ctcacttatt aaaacataaa taatttttta agcggaaatg taattatgag actgccagag
       61 aaagttcttt ttcctcctgt cactagtggc ctgtcagggc aggaaaaaca aaaaaaaccg
      121 aagagcatta ccggatttca ggaaaattat caacgcaata tcaggccaat caaaacagca
      181 tcagaagccc gactacgctt ctttgataaa atggtttcga aagaaaactc tctggaagat
      241 gttgtttctt taggtgaaat gattcagaag gaaatttatg ggcatgaaca aagaacattt
      301 tcaccagttc atcatacagg taactggaaa tcatcattgt tacacaacgc gctccttggt
      361 ctggcaaatg tttataatgg cttacgggaa acagaatacc ctaacacttt caacagagat
      421 ggtataaaaa gtactaactc ttttagagat aacttattga caaaaacaag aactcccaga
      481 gataattttg aggaaggaat aaaacatcct gaacatgcaa caataccata tgacaacgac
      541 aatgaaagta ataaattgct aaaagcagga aagatagctg gtaacaataa cgagctgttg
      601 atggaaataa aaaaggaatc ccaaagcgac catcaaatcc ccctgtcaga taagttcctg
      661 aaaaggaaaa aacgatctcc tgtagctgaa gataaagttc aaaactcgtt aacaccagaa
      721 aattttgttc
//
LOCUS       DAZGXS010000443          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGXS010000443 DAZGXS010000000
VERSION     DAZGXS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>729
                     /locus_tag="ACHKG5_005243"
     CDS             <1..>729
                     /locus_tag="ACHKG5_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871113.1"
                     /translation="IKTGNERISIGIRNRSNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEESFETALDVEHTIVLGFADDNFETK
                     PVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFM
                     SLPVPVATMVREDASNLINLKTLL"
BASE COUNT          255 a          110 c          135 g          229 t
ORIGIN      
        1 aaataaaaac gggtaatgaa agaatctcta ttggaatacg taacagatcg aatggaaaac
       61 ttgaaagaca aaagtttctt ttcccactgg actatgagaa taaattacaa catcaattag
      121 acaagcattt tacgattgaa tcgcacccat tattatacag gcatactatg ggtagtaaga
      181 ttgcgaatgt gatttttgaa aaattataca gtcgtattga ctttaacaaa gaacaatata
      241 tatcctttat aaaggacgcg tttcttcatt tttatgatta tagcaggcga tatgcaatta
      301 atgaaaatat tgcaaaagac gctgtaacaa acaatatcgc attgatgagc actttttatg
      361 acagtgataa tacgtcaggt gaggttttaa ataatgattt tacagaagag tcatttgaaa
      421 ctgcgttaga tgtagagcat acaatagtgc ttggatttgc tgatgataat tttgaaacaa
      481 aacctgttca ttatcaggat ttattaacac gatttagtgc ctttcaagat accgtgttta
      541 atctgtttcc tgaaatgcac tcttcgcatt acaacgatat ttgtagcgta agtgtggata
      601 tgacaaaggg aacgcaatgc atgattcact tgatggtgaa tgaagaagtt ttcatgtctc
      661 tgcctgttcc agttgccacg atggtacgtg aagatgcaag taatttaatc aatcttaaaa
      721 cactactca
//
LOCUS       DAZGXS010000444          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGXS010000444 DAZGXS010000000
VERSION     DAZGXS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>727)
                     /locus_tag="ACHKG5_005244"
     CDS             complement(<1..>727)
                     /locus_tag="ACHKG5_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871114.1"
                     /translation="CNNSIYDECIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNS
                     FYLFWVNPIRYRITQSWTGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTT
                     SVLALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMI
                     PVAKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNA
                     LCLLTEGQLNQNKKEMSDKLFRPT"
BASE COUNT          227 a          142 c          103 g          255 t
ORIGIN      
        1 ttgtaggtct gaataattta tctgacattt cctttttatt ttgatttaac tgtccttcag
       61 ttaacaagca aagcgcatta gaatatttga ttttcttctc tctatcatac caaaagagat
      121 caggaggtat attttcttta agggttccct tcgttgaatt agagtaattc ttttgaattt
      181 cagcagccaa taacctcgct tgacttgata attcttttaa tctgctaata gcctttttag
      241 ctacaggtat cattaaagtt ggaatccatt ttatattgcc ttcataccct tttgctgaaa
      301 aaaatcttaa accgtatctt tgaatgccct ttccatcaca ttctgtgatt tcacaatccg
      361 ctggcaaagc taaaatttcc gatatcctac tcggtgcaca cataagtaaa gcaagcacag
      421 atgtagtaaa tatatctctt gacgataatt gttcatcccg ctttgaaaaa atctcagcaa
      481 tcgcaataac tgatttgata tcaggcaatc tagaatggcc ttccagtgaa gaatcataac
      541 cagtccaaga ctgagtaatc ctatacctaa ttggatttac ccaaaataaa taaaatgagt
      601 tagtcatcct attatcattt aaaaatgaac tcaatttctc aagttctttc ccaatagcaa
      661 atgcatgtgc tttagaatac ttttcagagg ctatccttat gcattcatca tagattgaat
      721 tgttgca
//
LOCUS       DAZGXS010000445          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGXS010000445 DAZGXS010000000
VERSION     DAZGXS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            2..601
                     /gene="map"
                     /locus_tag="ACHKG5_005245"
     CDS             2..601
                     /gene="map"
                     /locus_tag="ACHKG5_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFP0871115.1"
                     /translation="MTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQSNFV
                     INHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVISKET
                     KGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFNTQTK
                     MIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          243 a          136 c          162 g          184 t
ORIGIN      
        1 aatgacaatg gcaggcagat cgttagttca ggcgactgca cagacgctta aacctgcggt
       61 taccagagct gcaatgcaag cgggtacggg agccacgggt atgaggttca tgcccgtgca
      121 gtcgaacttt gtgattaatc atggcaaact gactaaccaa ctcctgcagg ctgtagccaa
      181 acaaactcgt aatggcgata cccaacaatg gttccagcag gagcagacga cttatatatc
      241 cagaacagta aatagaactc tggatgacta ttgcaggagt aataattcgg tcattagtaa
      301 ggagactaaa ggtcacattt ttagagctgt tgaaaatgcg ttacagcaac ctcttgatat
      361 gaacggggca caatcatcaa ttggtcactt cttgcagtcc aataaatact ttaatcagaa
      421 agttgatgag cagtgcggca aaagagtgga tccaataaca cgttttaaca cccagacgaa
      481 gatgatagaa caagtctcac aagaaatatt tgaacgcaac tttagcggct tcaaagtcag
      541 tgagataaaa gcgattactc agaatgcgat tttagagcat gtacaggata cccgattgta
      601 gggataacct tgtcaggtat aaattaagct tcctggtgta tagcatggcc tttgttgatg
      661 taagaaaaaa cgcgataaag aaacttaata aactttatta ttaattaaga tttatagttt
      721 gctta
//
LOCUS       DAZGXS010000446          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGXS010000446 DAZGXS010000000
VERSION     DAZGXS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(36..686)
                     /locus_tag="ACHKG5_005246"
     CDS             complement(36..686)
                     /locus_tag="ACHKG5_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0871116.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          204 a          138 c          143 g          237 t
ORIGIN      
        1 attgaaaata tgcttcttat attgtgcttt tatttttaaa tactgttttg ttgaagtggg
       61 tatatgggta atgcgtaaaa acattgtttt gttatattaa aatgacactt gtctttgctt
      121 ataatcattt ctggagcaaa ggggtcacgg ctgagggggt ggagagcgtt acgcaggata
      181 agttcagtca atgcttcctg atcatataat gcacatattt ctgaatttcg agcatttctg
      241 acaaaaacac ctgtctcagg aatatgtagc gttatcggac aattaagatg ttcctcagta
      301 caggaaaagt cttgtaaact aacgggaaat gcattcgaat ttattcgatc tagtagtgtt
      361 tttatgacgc tctcctgcac aagcggggca gcttgcctct gctccagata taagctgtat
      421 gcctgcagaa aagtgttacc tccgttaagt tgtcttgtca ggcggtctgc gagatgatga
      481 gcctctcttg ccccaagatg gtcccaaagt ccaccaagaa taggctcaac actaaaacca
      541 tccataaact ggatataatg aagcctcata ttacggcctc ctatagtaat agtgtcattc
      601 tggttgcttc ttgctatatg tcgtaaagcc tccagctcat tttccggcaa tacacttcca
      661 ttagaaaaat ttaatatgac tggcatatta ttatcctttt taatccatat tgtgaactat
      721 at
//
LOCUS       DAZGXS010000447          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGXS010000447 DAZGXS010000000
VERSION     DAZGXS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..664
                     /gene="espW"
                     /locus_tag="ACHKG5_005247"
     CDS             <1..664
                     /gene="espW"
                     /locus_tag="ACHKG5_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP0871117.1"
                     /translation="KFTCIEDFYKRYNETRLKANTNIQQEQIADEVKSLTYLIPSEKK
                     EMWIYKNNGKDNAKPNLGERDVRMFENISSDDTDKITGRKFSELGEYLYSGNVIKLSQ
                     LSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNIKTML
                     NNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTWDPLV
                     RN"
BASE COUNT          291 a          122 c          120 g          183 t
ORIGIN      
        1 aaaattcaca tgcattgaag atttttataa aaggtataat gaaaccaggt taaaggccaa
       61 taccaatatc caacaagaac aaattgcaga tgaagtaaaa tcacttacat atttgattcc
      121 ttcagaaaaa aaagagatgt ggatatataa aaacaatgga aaagataatg caaaaccaaa
      181 cttaggagag cgagacgtaa gaatgttcga aaatattagt tctgatgata cagataagat
      241 aacaggaagg aaattttcag agttaggtga gtatctttat tcagggaatg taataaaact
      301 cagtcagtta tcaattcgtt acttacccaa tatcagctca atctcattaa tagagacaaa
      361 acagagtttg ttgctacatc gattgtattc agatgaagta cttcagagaa atggaacgct
      421 tatcccgaca ccactacatg aagaaaaatc aattccagct gacaatataa aaacaatgct
      481 caacaacata ccgacttaca aaatgttacc gccattcaca gaaacacaag gtaattgttc
      541 ttctggcgca gccacgtttt tacgcaaatc aggcgccgaa gaaaaagata ttcttgcatg
      601 tagcccccga aattatgggc tgcatcataa cataaaaaca tgggacccct tggttagaaa
      661 ttaaggatcc aaatattata tatttcatat aaagcaagaa aaaaatattg tctgaa
//
LOCUS       DAZGXS010000448          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGXS010000448 DAZGXS010000000
VERSION     DAZGXS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..471)
                     /locus_tag="ACHKG5_005248"
     CDS             complement(<1..471)
                     /locus_tag="ACHKG5_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP0871118.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLP"
     gene            complement(504..581)
                     /locus_tag="ACHKG5_005249"
                     /pseudo
     CDS             complement(504..581)
                     /locus_tag="ACHKG5_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(583..>714)
                     /locus_tag="ACHKG5_005250"
     CDS             complement(583..>714)
                     /locus_tag="ACHKG5_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0871119.1"
                     /translation="PEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          202 a          210 c          115 g          187 t
ORIGIN      
        1 gggtaagtct tcaccagtca aacgagaaaa tgcagcggca tcaaataaag tacatacatc
       61 tgaaccatct gaattcttca caaaaacacc tttttcaggc tgctccagtg taattggaca
      121 ttgaagcgca tcaccaggac attgaagttt ttcaatatcg acagtaaagg cacactccga
      181 tattttttcc tgcaaagagt ttcgccctcc cattccaggg agcgcactta atatcctgtt
      241 tctgagcaca ttaactccat cattaaaagc aatattcaat aattctgcat aaacccgtcc
      301 ctgcccttca ctttctgcta aaaaacggcc tgtatctggc atataagaaa ctctaaccct
      361 ctcccctccc aggagaaatc caccaatctg cccctccatt acctgctgac gcagctccat
      421 catctcattc acgaatgaag ccgatgacac atatgatatt aatccaggca ttaccatgct
      481 ccttcatacg gataaatatt ccctcaagtc ttcattaaca aacatccacc ggacatgaca
      541 acaaaaaccg gagccggact ccggttttgt gaagctgtcg ggttacttca tcccgccaat
      601 attttcccac gtcccgtcag cacgcaggat ttgcagcggt cttaccacac actgtatctg
      661 ctttttatcc gcatccagta tcaccacctg cgtgattacc ctgacctgct ccgg
//
LOCUS       DAZGXS010000449          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGXS010000449 DAZGXS010000000
VERSION     DAZGXS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..589)
                     /locus_tag="ACHKG5_005251"
     CDS             complement(<1..589)
                     /locus_tag="ACHKG5_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFP0871120.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          208 a          166 c          152 g          184 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccagaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcacgtattt attcgagaac aatgctgata acaatcaaat
      661 aattagtcgt ttaaaaaaac gtgagattat taaattatta atgcattcca
//
LOCUS       DAZGXS010000450          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGXS010000450 DAZGXS010000000
VERSION     DAZGXS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..551)
                     /locus_tag="ACHKG5_005252"
                     /pseudo
     CDS             complement(1..551)
                     /locus_tag="ACHKG5_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          243 a          125 c          101 g          227 t
ORIGIN      
        1 gtttttgttt acaagaatct tatcgatatg tctcagctgc ggatcattac tggcattaac
       61 aatataaaaa gcaatgaaaa tgataacaaa aagaaaaaaa agcattacat actttaacag
      121 tttactgcta aagaaatgtt gttttgtaga aataatgcta ttttcaataa tgcctggcaa
      181 tgaatactgc caaacttcat tttttctttt acagcaaatt tgagacctaa aacttctatt
      241 tgctgattca atgttaacgt tctatcttca ggttcttgtg ctgttaattc tctgattgtt
      301 acaaccagat cagggtcggt agtgattata atctcaaaac tacctgtaag ctcgccataa
      361 ggaacgacta ttgtattttc tggtaactca gaaaaagcat cacttcttcc taacgaaaca
      421 tcattaccaa tgataacaag tagacgccca ttaagtattt catactcgca tccattctat
      481 ggtccgctca acaaacgaat ggcataagat tccaataagt cttgtgataa gaatttatca
      541 ttattttcca tgaataccac ccgctaattc cataagctct agtttagagt agtgtaacaa
      601 gacggaaatt tcatgaccaa attatgatga gacagaataa aaaaattata attttgagga
      661 ccattttaaa tgcctctatg cataacttta tttaca
//
LOCUS       DAZGXS010000451          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGXS010000451 DAZGXS010000000
VERSION     DAZGXS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>691)
                     /gene="espX2"
                     /locus_tag="ACHKG5_005253"
     CDS             complement(<1..>691)
                     /gene="espX2"
                     /locus_tag="ACHKG5_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP0871121.1"
                     /translation="VNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHILRTVNSL
                     NVGEKLNDDNLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSKVKDIVD
                     DMSNHDVESEKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGMELFALR
                     DIVAFPSTIIAKLIKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFHALFLKR
                     PELMVAENYRFI"
BASE COUNT          209 a          130 c          112 g          240 t
ORIGIN      
        1 aataaatcta taattttcag caaccatcaa ctctggtctc tttaaaaaga gggcatggaa
       61 aaagttaatt atttccgcat tgttattcag atttttattt ctgaagtagc acagatacgt
      121 atccagtgcg cgcataacaa gttcatgatc gctattaagt ggggatttta ttaattttgc
      181 tattatggtt gatgggaatg ccactatgtc ccgcagcgcg aagagctcca tgccatcagt
      241 taattgcgtt aacagatctt ttactgtttg tatgttatca gtactatcat tgaaagattc
      301 gtctgccaga gaattcagtt tttctatcat ccccccgatt acaaatatat ctttttcgct
      361 ctccacgtca tgattagaca tatcatcgac gatatcttta actttactat cactttcaaa
      421 tagtttttct ctcacaatat tattagcgtt tactgcagaa gttgcatcat aataggatgg
      481 cggaataata acagagaatt cgttagtgaa tatagaatga tctgcaagat tatcatcatt
      541 caatttctcg ccaacattta gcgaattaac agtcctcaga atatgcatct caatctgact
      601 gtcagaaaga cttttagaaa aaccactacc ttcagtcagt tcaattttca tcctgagtat
      661 tttatttttg aggtcgggaa atgtatttac a
//
LOCUS       DAZGXS010000452          687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGXS010000452 DAZGXS010000000
VERSION     DAZGXS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>687
                     /locus_tag="ACHKG5_005254"
     CDS             <1..>687
                     /locus_tag="ACHKG5_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0871122.1"
                     /translation="DEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGA
                     DFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWVH
                     ETAVVRRKSEVSRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSG
                     VGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNA
                     RVEPKGNAIL"
BASE COUNT          131 a          150 c          262 g          144 t
ORIGIN      
        1 tggatgaggc ctttctgtac cgggagtacc gtaaagcccg ggaggctggt gaggaagcat
       61 ttcgtggctt catgtcaaaa cacgccaatg tggaaatcgg tcttgccctg cgttctgacc
      121 gctgggcggg cgcggatttc tgggagcagc agggcaggcg cgtcagcctg gacgatatcc
      181 tgcagcgcgc tgatgtggtg acggtgggga ttgacggcgg gggcctggat gatctgctgg
      241 gaatgtacgt gactggccgt gacagggaaa cccgcgaatg gctgggctgg ggccatgcct
      301 gggtgcatga aaccgcggtg gtcagacgga agagtgaggt atcccggttt caggattttg
      361 tggcctgtgg agacatgacg attgtccgtc gggtcgggga tgacacggcg gaagtggcgg
      421 agtatgtgcg tcgtattcat gaggctgagt tactggatca tatcggtatt gacccgtcag
      481 gtgtggggca gattctggat tcactggcgg aagccgggat ccccgatgag agtgtggtgg
      541 ggataagcca gggctggaag ctgggcgggg ccatcaaaac caccgagcgc aaactggctg
      601 agggagtgct ggtgcatggt ggtcagccac tgatggcctg gtgcgttggc aatgcccggg
      661 tggagcctaa aggtaacgcc attctta
//
LOCUS       DAZGXS010000453          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGXS010000453 DAZGXS010000000
VERSION     DAZGXS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..347
                     /gene="sctQ"
                     /locus_tag="ACHKG5_005255"
     CDS             <1..347
                     /gene="sctQ"
                     /locus_tag="ACHKG5_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HFP0871123.1"
                     /translation="KMADHFQYEEDFETDDFDIKKSESEIYDENDEQMINSFEELPVK
                     IEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVE
                     IHSWLSGHNNVK"
     gene            337..>686
                     /locus_tag="ACHKG5_005256"
     CDS             337..>686
                     /locus_tag="ACHKG5_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0871124.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTSFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFE"
BASE COUNT          225 a           85 c          150 g          226 t
ORIGIN      
        1 taaaaatggc tgatcatttt cagtacgagg aagattttga aacagatgac tttgatatca
       61 aaaaaagcga gagtgagatt tatgatgaaa atgacgaaca gatgattaat agttttgaag
      121 agttgcctgt aaaaattgag tttgttcttg gtaagaaaat aatgaatcta tatgaaatag
      181 acgagctctg tgcaaaaaga ataatatctc tgcttcctga atctgagaaa aatatagaga
      241 tacgtgtaaa tggcgcgcta actggctatg gcgaacttgt tgaagtggat gataaattgg
      301 gcgtagagat tcattcttgg ttatcagggc ataataatgt caaatagcat ttcgctgata
      361 gcgatactat cattatttac actattgccg tttataatag catccgggac aagttttatt
      421 aaattctcga ttgtttttgt tattgtccga aacgcattgg gacttcagca agtgccatca
      481 aatatgacac ttaatggggt tgctttgcta ttgtcaatgt ttgtaatgat gccggttggg
      541 aaggaaatat attataacag tcagaatgag aatctttcat tcaataatgt agcgtcggtt
      601 gttaattttg ttgagacagg catgagtggt tataaatcat atttgataaa atattctgaa
      661 ccggagttgg ttagcttttt tgaaaa
//
LOCUS       DAZGXS010000454          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGXS010000454 DAZGXS010000000
VERSION     DAZGXS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..478
                     /gene="espX1"
                     /locus_tag="ACHKG5_005257"
     CDS             <1..478
                     /gene="espX1"
                     /locus_tag="ACHKG5_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP0871125.1"
                     /translation="EKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSN
                     IFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILI
                     PLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          259 a          121 c           99 g          192 t
ORIGIN      
        1 agaaaagagc aacctttcat atgggtatta ttttggttgt gtattatcca atatatcatg
       61 ttttgaaagc gacttatcaa acaccatctt ttcaaatgga gaaataaaca acctcttcat
      121 taaaaaaagc aacatatttg gaacatcgtt cactaatacc atgataaaaa atttgcgctg
      181 tgaagatatt atgcctggcc gatggaccac tcaacttgtc aataagcact taggttacag
      241 atacacagga gtattcaaaa ccctggcaag tattgatgac aaaccgtccc gtttcgaaat
      301 tttgatacca ttggtacaaa ctttagttcg cgacaatgta aagctaaata atgatgtgta
      361 taaagaactg aaaaagttta tgcacgatta cgacaaaaca tccccagaaa tgagaaaata
      421 ccttcagagc atcaacgagt ctatgttcct tatgaaaaaa atatctcatc aagattaaaa
      481 caccccaaaa aagaagtaat ttcagaatga aattacctgt gataattatt aattaactgc
      541 agagaaataa aaaataatct gaagcatgct ctgaaacatc ctactctgtc actctaatga
      601 tggcaccttg ctcttcttat aaagaaggga ataattaaga atattaaatt ttcaaacttt
      661 catcaacttc a
//
LOCUS       DAZGXS010000455          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGXS010000455 DAZGXS010000000
VERSION     DAZGXS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..235)
                     /gene="grlR"
                     /locus_tag="ACHKG5_005258"
     CDS             complement(<1..235)
                     /gene="grlR"
                     /locus_tag="ACHKG5_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFP0871126.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKK"
     gene            431..>654
                     /gene="etgA"
                     /locus_tag="ACHKG5_005259"
     CDS             431..>654
                     /gene="etgA"
                     /locus_tag="ACHKG5_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFP0871127.1"
                     /translation="MKKIILSIILISNYCYASDCFEITGKAYNIDPLILKAIAWNESK
                     CKSGIKSKTNKNGTYDIGIMQINSSHLDLL"
BASE COUNT          235 a          117 c           89 g          213 t
ORIGIN      
        1 cttttttagg gataactaat tgataatctt gttcaatgtt tagcaccgag ggaatttcgt
       61 aattatatcg atggacatga agtatgatgt cctcatcttc agagaggacc ccctgataca
      121 cagaagcaat atcgccgcca gtgatcatat ttccattttt aatcagtata ccttccccac
      181 aggagtcttc attgctaata aatataatgc tatagatgcc atccttcata atcataactc
      241 actcagtttc atactaacct cactcctttc aatttgttct atccataagc aatatcaaga
      301 ataatggaga cctttctttt tctccagatt gcaatgtaat atatcattaa atatactcat
      361 gtttcaacct gctgtttccc tggttaacat atgtaatttc tgattaataa tattgagtaa
      421 caacgtaaaa atgaaaaaaa taatactgag catcattctc atttcaaatt actgttatgc
      481 ttccgattgt tttgaaatta caggaaaagc atataacata gatccattaa tattaaaagc
      541 aattgcatgg aatgaatcaa aatgcaaaag cggaataaaa agcaaaacaa acaaaaacgg
      601 aacgtatgat ataggtataa tgcaaattaa ttcatctcat ttagatttat taag
//
LOCUS       DAZGXS010000456          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGXS010000456 DAZGXS010000000
VERSION     DAZGXS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>648
                     /locus_tag="ACHKG5_005260"
     CDS             <1..>648
                     /locus_tag="ACHKG5_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0871128.1"
                     /translation="TPYTLMQAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGL
                     PLPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLS
                     KGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGIFSESTGASQDSA
                     RWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAML"
BASE COUNT          143 a          168 c          203 g          134 t
ORIGIN      
        1 accccttata ccctgatgca ggcgattaca gcagaaggtg atgtggtggt cagtggtgca
       61 actgagccgg attggtacta cgtcatcgta ctggccgggc agtccaatgc catggcttac
      121 ggtgaagggc ttccgctgcc ggattcatac gatgctccgg atccgcgcat taaacagctg
      181 gcgcgccgca gtacagtgac gccgggtggg gctgcctgca gatataacga tattattccg
      241 gccgaccact gcctgcatga tgtgcaggat atgagtacgc tgaatcatcc gaaggcagac
      301 ctgagcaaag ggcagtacgg ctgtgtcggc caggggttac atattgccaa aaaactgctc
      361 ccgtatatcc cgaataacgc ggggatcctg ctggtaccat gctgtcgtgg tggttcggca
      421 tttacccagg gcgcggaggg gatattcagc gagtccacgg gggccagcca ggattcggcg
      481 cgctggggtg tgggtaaacc gttatatcag gacctgattg cgcgcaccaa agctgcatta
      541 cagaagaacc cgaaaaatgt gttgctggcg gtgtgctgga tgcagggaga gtttgacatg
      601 agcgccgcca cccacgcaca gcaacctgcg ctgtttacag ccatgctg
//
LOCUS       DAZGXS010000457          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGXS010000457 DAZGXS010000000
VERSION     DAZGXS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            9..104
                     /locus_tag="ACHKG5_005261"
     CDS             9..104
                     /locus_tag="ACHKG5_005261"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFP0871129.1"
                     /translation="MEGKQPKNSGGNFTMLIAKKDGQVLNVIHTK"
     gene            complement(460..>647)
                     /locus_tag="ACHKG5_005262"
     CDS             complement(460..>647)
                     /locus_tag="ACHKG5_005262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871130.1"
                     /translation="PPYSKNTQAFRTLYQPHLDCFPFPDTAARGVIKVRNFQQYGVLL
                     FTAVMHQPVLAIVVITV"
BASE COUNT          208 a          124 c          143 g          172 t
ORIGIN      
        1 gttggaaaat cgagggaaaa caaccgaaga attcaggagg aaattttaca atgctaatag
       61 cgaaaaaaga tgggcaagtg ttgaatgtaa tacacacaaa ataatccact gtacagtcga
      121 ttcaaatatt ttaccaaggt gtgtttcgta acaattttgt gtacaatatg actctacata
      181 tcgacgaaaa aaccacttga tttgacagga atacattcaa aaaatttctt ttttcatttc
      241 aggagcgatt ttcatctgtt acttgaagta tctgtgagat taagtgactc cccctaacgt
      301 cgtactaaat agtaattact ggccataccg ggagcagtat tcggcgctcc ccacatgcag
      361 cagaataacc gcatcagcga agatgtattt ttactatcac aatgtgtacg gacggtttat
      421 ggaaaagaat gaacattaga tacgcgatgc ggcggcaggt tacacggtta tcactacgat
      481 agccaatact ggctggtgca ttaccgccgt gaacaatagg acgccgtact gctggaaatt
      541 tcttaccttt ataactcccc gcgccgccgt atcgggaaac gggaagcaat caagatgagg
      601 ctgatataat gtgcggaatg cctgagtatt tttagaatag gggggta
//
LOCUS       DAZGXS010000458          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGXS010000458 DAZGXS010000000
VERSION     DAZGXS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            87..449
                     /locus_tag="ACHKG5_005263"
     CDS             87..449
                     /locus_tag="ACHKG5_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0871131.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          197 a          151 c          150 g          144 t
ORIGIN      
        1 aactattgca atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata
       61 aatttatctt tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaaacgtc
      121 tcagcgatcg tctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg
      181 cagacaaata tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc
      241 tgcgtgaagt tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt
      301 ttcagcgcgc aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc
      361 gcggactggt ggttgtgcac ccaatgaccg cgctgggccg tgaaatgggc ctgcaggaga
      421 tgactggatt ctctaagact gcgttttaag cgcctcagac gactggttcg atcatatcaa
      481 cccgcatctg gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt
      541 accaaacaat tcactccctt tacgtcgatt taccgctggt tgatattctg aaaatgcatt
      601 tttaccgaaa tagccaccta taaacaataa aaatatatag aa
//
LOCUS       DAZGXS010000459          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGXS010000459 DAZGXS010000000
VERSION     DAZGXS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..103)
                     /locus_tag="ACHKG5_005264"
     CDS             complement(<1..103)
                     /locus_tag="ACHKG5_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871132.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKP"
     gene            165..641
                     /locus_tag="ACHKG5_005265"
                     /pseudo
     CDS             165..641
                     /locus_tag="ACHKG5_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          178 a          158 c          155 g          150 t
ORIGIN      
        1 cgggttttga acccaggata ttcgctattt cactgaagcc tgttccgttc ttccatagtt
       61 caaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa acctccgcaa
      121 ccccatgttt tcacataact gttgcgttga ccaattgaat ctacaacagc gtattttgca
      181 caggagtcgc tgaaaaatac gcgttaatcg aacaatggcg acaacaattt cccattgaag
      241 cgatgtgtca ggtatttggt gtatccagga gcggttatta caactgggta cagcatgaac
      301 cctcagacag aaaacaaagt gatgagcggc taaaactgga gattaaggtg gcacatatcc
      361 gcactcgcga aacatatgga acccggcggc tccagacgga gctggcagag aatggcatca
      421 tcgttggtcg tgaccgactg gcacgtcttc gtaaggagct aaggctacgc tgtaagcaga
      481 aacgcaagtt cagggcgact acgaactcga accacaatct gccagttgcg ccaaatctgc
      541 tgaaccagac gttcgctcct acagcaccaa atcaggtctg ggtggcggac ctgacgtatg
      601 ttgccacaca ggagggatgg ttgtacctcg ctggcatcaa a
//
LOCUS       DAZGXS010000460          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGXS010000460 DAZGXS010000000
VERSION     DAZGXS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>636
                     /locus_tag="ACHKG5_005266"
     CDS             <1..>636
                     /locus_tag="ACHKG5_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0871133.1"
                     /translation="LDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDR
                     LTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPET
                     GELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRL
                     HRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGDLVRIS"
BASE COUNT          149 a          183 c          212 g           92 t
ORIGIN      
        1 cgcttgaccg gctggtacag cagcgcggct ttgacgggcg gacgcaacgt taccactatg
       61 acctgaccgg aaaactcacg cagagtgaag atgaggggct tgtcaccctc tggcactacg
      121 acgaatcgga ccgcctcact caccgcacgg tgaacggcga accggcagag cagtggcagt
      181 acgacgagca cggctggctg acagaaatca gccacctgag cgaaggccat caggtggcgg
      241 tgcattacgg ttatgatgat aagggccgcc tggccgggga gcgccagacg gtgcataacc
      301 cggagacggg ggaactgctg tggcagcatg agacagagca cgcatacaac gaacagggtc
      361 tggcaaaccg cgtcacgccg gacagcctgc cgcgggtgga gtggctgacc tacggcagcg
      421 gttatcttgc ggggatgaag ctgggcggga cgccgctggt ggagttcacg cgcgacaggc
      481 tgcaccgcga gacggtgcgc agcttcggca ataacgcata cgaactgacc agcacataca
      541 ctcccgcagg ccatttacag agccagcgcc tgaacagcca ggtgtatgac cgtgactacg
      601 actggaatga caatggcgac ctggtgcgca tcagcg
//
LOCUS       DAZGXS010000461          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGXS010000461 DAZGXS010000000
VERSION     DAZGXS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            139..564
                     /gene="yqeK"
                     /locus_tag="ACHKG5_005267"
     CDS             139..564
                     /gene="yqeK"
                     /locus_tag="ACHKG5_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFP0871134.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          213 a           85 c          122 g          204 t
ORIGIN      
        1 caaggttatc accttatgga gacgttacgt agaatgacta tatttgttat cggcacatta
       61 ggggtattaa ttttagtatt gatggaaggt gattattgaa cagtaacatc gagtcacggt
      121 aaaaatgtga ggtatataat ggacattgag ttttcgcaga ttcatgaaat ggtctatatg
      181 catgatattg taaattcgga ctcgaaaaag aaaccaagga ttccgttaaa aaaattcctt
      241 aatgctgaga aggttctcac gcaaacaaca tcatgggcat taaattcaag atttgtgaat
      301 gttaattctg tcaataaagt caacgtaaag agtaaagtta aaaacagtta tatctcacga
      361 tctgtcaatg atgagtttag tttaacagat gatgaaatca attcttttaa agaaacgtta
      421 gtcctttcct ctattgattc actgagtaaa ctggttctta ataatcctct ttctgtattg
      481 tttacatcga ctgtacgaag gaataataat cgagctaaga tgaatgtcga gttcgactca
      541 tggatttgca ccagatgttg ttgatacatg atattttaca ttgtaaggag atgtctaaaa
      601 tgaaagatgt tgatcaaaac tttg
//
LOCUS       DAZGXS010000462          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGXS010000462 DAZGXS010000000
VERSION     DAZGXS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
BASE COUNT          215 a          109 c          123 g          176 t
ORIGIN      
        1 ttcctctgaa acagagaggt tcaagataaa gtatcaacaa gagaatacac ttaatgatgt
       61 agttcaaaat atcaggactt ggctaaaaga aaatcaaaat atggtgaaag ctattaagaa
      121 aattgatact gaataacatg gcgaataaat aaacggatca agtcgttcct atgacaatga
      181 ggttatacga aaagacgctt actcatcaga aatctggtat gctttaatta aaccagtgtc
      241 gctgatgtgt aagcgttgac gttgggagac gattaaatgg ttataccact cctacgtctt
      301 ttccatacgg cgtataagag tgcaacatgt tgagttttcc gagtgaatgg tgcattttac
      361 taaccgagcc atttgaatca accaacgcaa taaaaaagac tggaataata agcctgtatt
      421 tattatttat cgcaatcgca ctttacggtt ttatccaact gaaaagagac tgcttccagc
      481 ggttgtggat tatccctggg cgatagtcat agtctggtgt caggattcac aaatgacctg
      541 tttccttaca tggatataaa cacaacatag agtgcggtag ttaatctgaa taatctaatt
      601 tcggctattc agcacaaaac aac
//
LOCUS       DAZGXS010000463          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGXS010000463 DAZGXS010000000
VERSION     DAZGXS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>622
                     /locus_tag="ACHKG5_005268"
     CDS             <1..>622
                     /locus_tag="ACHKG5_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFP0871135.1"
                     /translation="INELKMESETARKEYQLFRQGNYDKYITTDIFEEHGLIKYVNLS
                     FDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLSPLFFNEYDSG
                     PIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNRLIRPSLGNRP
                     GTGRLFHYTSVDFKNKFSLKPTPLKIIQISSTVKKFKVTFYDENTNL"
BASE COUNT          223 a           87 c          116 g          196 t
ORIGIN      
        1 tatcaatgaa ttaaaaatgg agtcagaaac agcaagaaaa gaatatcaac ttttcagaca
       61 aggaaattat gataaatata tcactactga tattttcgaa gaacatggtt tgataaaata
      121 tgtgaactta agtttcgata ttgttgcatc tgtaggacag gtcgtaggcg gggttggcgc
      181 gttgaaattt ggtaaggtag tgcattcaaa ccgaataaaa ggtataggcg tcacattagt
      241 tgctcatggg gcgaataatt tttatgaaag cttatcacct ttatttttta acgagtatga
      301 ttcaggtcca atacgtgaac tctacagagt aatagcaaaa aaaatgggag gtgatattaa
      361 ttctgctgat tatgcataca gcatcgtgga cttttcgatt actgcatatg gtggatattc
      421 aggcatgaaa atcgtaccta agtataatag attgattcgt ccttcattag gaaatagacc
      481 tggtacagga cgattatttc attacacatc agttgatttt aaaaataaat tctcattaaa
      541 acctacccct ttaaaaatca tacaaattag ttctactgta aaaaaattca aggttacatt
      601 ttatgatgaa aatacaaatt tg
//
LOCUS       DAZGXS010000464          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGXS010000464 DAZGXS010000000
VERSION     DAZGXS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            66..>605
                     /gene="escD"
                     /locus_tag="ACHKG5_005269"
     CDS             66..>605
                     /gene="escD"
                     /locus_tag="ACHKG5_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP0871136.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALY"
BASE COUNT          172 a          126 c          124 g          183 t
ORIGIN      
        1 tagacgatgt aagttcacca tattttttct cattgttcaa ccaccaggat gaataaaatt
       61 taaaaatgtt atcctcatat aaaataaaac ttctcaatgg tgcgatgcgt aatcgggaat
      121 tacaacttcc gatgggaaac ctaacgctag gtacggaaga taacgatatt gtttactttc
      181 cccttgaaca aggcctcaat cagtttttac ttgatatacg tgaggaaggt gttttccttt
      241 tatctccggt tgaattttgg attgatgggc aacccacgcc ctatgaagca gataaaccat
      301 tacctgtcgg aaaggttatt gatatagcgg gatgttgctt catcattggt gatattgacc
      361 attccttgcc attgtctgat gtgccagaaa ggttctctgc caaaagtcga cgcaaaaaac
      421 gccttatcct ggcgagcgtg attggtgcta cttttgcgtt gtctggtgcc atcggcagtt
      481 acgttttgct ttccccaaaa gctgaacccc caacgtttac cagagctgat gtatatcagc
      541 agctaaaaga aaacaaactt catgcaatca ctctggtatg gcatgggaaa aatatcgcgc
      601 tttat
//
LOCUS       DAZGXS010000465          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGXS010000465 DAZGXS010000000
VERSION     DAZGXS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>603
                     /locus_tag="ACHKG5_005270"
     CDS             <1..>603
                     /locus_tag="ACHKG5_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0871137.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHC"
BASE COUNT          136 a          176 c          199 g           92 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cga
//
LOCUS       DAZGXS010000466          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGXS010000466 DAZGXS010000000
VERSION     DAZGXS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..513
                     /locus_tag="ACHKG5_005271"
     CDS             <1..513
                     /locus_tag="ACHKG5_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0871138.1"
                     /translation="LKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDGLNFSSKDLTNSYMNGMGKKECSSYLGTPNNTYQKYNPKAKGN
                     GVTIIRQGSN"
     gene            515..>597
                     /locus_tag="ACHKG5_005272"
     CDS             515..>597
                     /locus_tag="ACHKG5_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFP0871139.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMSDDNI"
BASE COUNT          227 a           75 c          128 g          167 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatgg catggatcca
       61 ttaggattat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      121 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      181 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      241 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      301 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tggtctaaat
      361 ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa agaatgcagt
      421 agttacttgg gaacaccaaa taacacctat caaaaatata atcccaaagc taaagggaat
      481 ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat gttgttattt
      541 ccttgcttta gtctccagtc atttaatggc tggagttatg agtgatgaca atatatt
//
LOCUS       DAZGXS010000467          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGXS010000467 DAZGXS010000000
VERSION     DAZGXS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>595
                     /gene="espX5"
                     /locus_tag="ACHKG5_005273"
     CDS             <1..>595
                     /gene="espX5"
                     /locus_tag="ACHKG5_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP0871140.1"
                     /translation="LSLFKMDDHEKVIDLIQKMKRIYDSLPSGKITKETDRKIHKYFI
                     DIASYANNKCDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSH
                     LSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSYF
                     AQIKMTDGTLNSCSAMHVQFYNATMNRANIKNTFLDYS"
BASE COUNT          226 a           92 c           95 g          182 t
ORIGIN      
        1 ccctctctct tttcaaaatg gatgatcatg aaaaagtgat agacttgatt cagaaaatga
       61 aaagaattta tgatagttta ccatcaggaa aaatcacgaa agaaacggac aggaaaatac
      121 ataaatattt tatagatata gcttcatatg caaataataa atgtgacgat agaattacga
      181 gaagagttta ccttaataaa gataaggaag tgtcaattaa ggtggtatat tttataaata
      241 atgtcaccgt ccataataat actatcgaaa tcccacagac agtgaatggt ggttacgatt
      301 tttcacacct tagcctgaaa ggtatcgtga ttaaagatga agatttatcc aattcgaatt
      361 ttgcaggttg cagactacaa aacgctattt tccaggactg taatatgtat aaaacgaatt
      421 ttaatttcgc cataatggaa aaaatacttt ttgataattg tattctcgat gactcatatt
      481 tcgctcagat aaaaatgact gacggaactc taaattcatg ctccgctatg catgttcaat
      541 tctacaatgc aacaatgaat agagccaata ttaaaaatac cttccttgat tattc
//
LOCUS       DAZGXS010000468          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGXS010000468 DAZGXS010000000
VERSION     DAZGXS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>592
                     /locus_tag="ACHKG5_005274"
     CDS             <1..>592
                     /locus_tag="ACHKG5_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871141.1"
                     /translation="EQRKVIFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMIL
                     ALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANA
                     HFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDK
                     VQVACYYLTILLAFERRTSLTSTLATLYKMLISEREV"
BASE COUNT          221 a          105 c           93 g          173 t
ORIGIN      
        1 gaacaacgaa aagttatatt taggagatat gaagatctct atgttaagct aatgcactat
       61 tctgttttta cagacaaaac acatcaaata ataaaacaaa aatattttaa tgacattgta
      121 ccaatgattc tcgcactcga catcaggaac acatatcgcc cggataatga gatggcattt
      181 tactatcata ttcattcttt tctcactcag ataccggata atgaggatga tatatatcat
      241 gctgcaagga catatctgcg aaattacgtt aagttatgtt tatccggata cacgccagcg
      301 aatgcgcatt tcaaagatat ctttgatggc gtatatgaat tcattcgtaa tattcgcaaa
      361 aacagtacac caggaaaaac aaaacttatc gcaactatca acacatgcaa agaaacctgt
      421 aaacatctgc tttatttaag taatgaagac aaggaaaaaa taatttctga cttagataaa
      481 gttcaggttg catgttatta tctcactata ttactggctt tcgaaagacg aacttcatta
      541 acaagcaccc tggcaacttt atataaaatg ctgataagcg aaagagaagt tt
//
LOCUS       DAZGXS010000469          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGXS010000469 DAZGXS010000000
VERSION     DAZGXS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            1..575
                     /locus_tag="ACHKG5_005275"
                     /pseudo
     CDS             1..575
                     /locus_tag="ACHKG5_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
BASE COUNT          214 a           99 c          112 g          163 t
ORIGIN      
        1 cagaaaataa tcacaattat caaactgaaa tgttgcttcg atggaactat cctgccgcct
       61 cagacctgct caggatgtat atccttaaag agcatggtgg tatttataca gacacagata
      121 tgatgcctgc atactctaaa caagtaattt ttaaaattat gatgcagaca aacggagata
      181 atcgtttctt ggaagatttg aaactacgtc gtgcaatatc tgatggtgta ttaagatatg
      241 ttaataacca aaatattgat gaagttaact ataatgaaat cagtgatgca gataaaaaca
      301 ttatcaagaa gatattaaca gaaatatcta aaatgccaga agatagtatt tttactaaga
      361 tcaatacaag gattcctcga gacacaatgc ccatccttcg tcgttatcac ctatggcctg
      421 atggatggaa tattcgtggg ctcaatggat tcatgctatc acataaaggt agtgaagtga
      481 ttgatgctgt tatcgcaggc cagaatcagg cttacaggga actaagaaga ataagagata
      541 atattcatag tgaaatatac ttcaaacaaa ctgattgaac cgccccgg
//
LOCUS       DAZGXS010000470          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGXS010000470 DAZGXS010000000
VERSION     DAZGXS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>588
                     /gene="espR4"
                     /locus_tag="ACHKG5_005276"
     CDS             <1..>588
                     /gene="espR4"
                     /locus_tag="ACHKG5_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFP0871142.1"
                     /translation="LLFENYEAYDKTGSITIEKNQGEGTLPIRHKLEFISTNIAELLD
                     KLTKITDARLCKGFSDWASSVKEGGSNDLKENVDRALVRMFKCVKLHSNELDLSYLFL
                     GSVPPLPDWIEMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITLISVNNNL
                     ISHIDSFPPKAKKIFISHNKLSETPAIPDTAKVFDC"
BASE COUNT          192 a          105 c          119 g          172 t
ORIGIN      
        1 ctattgtttg aaaattatga ggcttatgat aagacggggt ctatcacgat agaaaaaaat
       61 cagggggagg ggactttacc cattcggcat aaattagagt ttatatcgac taatattgca
      121 gagttgctgg acaagttaac gaaaattaca gatgccagac tttgcaaagg cttcagtgac
      181 tgggctagtt cagtcaaaga aggcggatcg aatgacctca aagaaaatgt agatagagcg
      241 ttggtgagaa tgtttaaatg tgttaagctt cacagtaatg aacttgactt atcataccta
      301 tttttgggtt ctgtgccgcc tcttccagat tggattgaaa tgcttagcct tgtttataat
      361 gaacttgatt caatacaggt tcccgaatca tgcaaagaat tagaactcga tttcaataac
      421 cttacagaat ttccacaagt acctgatgga attaccctga tctccgtaaa taataacctg
      481 atatcgcata ttgactcatt tccgccaaag gctaagaaaa tttttattag tcacaataag
      541 ctatcggaaa caccagcaat accagacacc gctaaggttt ttgattgt
//
LOCUS       DAZGXS010000471          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGXS010000471 DAZGXS010000000
VERSION     DAZGXS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>580
                     /locus_tag="ACHKG5_005277"
     CDS             <1..>580
                     /locus_tag="ACHKG5_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871143.1"
                     /translation="IGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQTSIEMV
                     TLLNTLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELT
                     INEPLKNQEISTGNININCPLKDIYNEFRRLNVIFSCGTGDIVDLSSLDLRNVDLDYY
                     DFTDKHMANTILNPFKLNSTNFTNANMFQVNFV"
BASE COUNT          203 a           96 c           91 g          190 t
ORIGIN      
        1 attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct acctgaaagc
       61 ccaacaggaa ataacctcga agcactagca ccaggtattg aaaaactaaa acagacctct
      121 attgaaatgg tcactttact taacacgtta caacctggtg gaaaatgcat tatcactggt
      181 gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt
      241 cgtctggatt ttttaggtta taacgcccaa attattcaac gatcggacaa tacttgtgaa
      301 cttaccatta atgaaccgtt aaaaaaccag gaaatatcca caggtaatat caatattaat
      361 tgcccattaa aagatattta caatgaattc aggaggttaa acgtaatttt tagttgtggg
      421 actggagata tcgttgatct atcctctctg gacttacgta atgtcgattt agattattat
      481 gatttcacag ataaacatat ggctaatact attttaaatc cttttaaatt gaattcaaca
      541 aattttacta atgccaacat gtttcaggtt aattttgtta
//
LOCUS       DAZGXS010000472          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGXS010000472 DAZGXS010000000
VERSION     DAZGXS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            254..>559
                     /locus_tag="ACHKG5_005278"
     CDS             254..>559
                     /locus_tag="ACHKG5_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP0871144.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     "
BASE COUNT          179 a           95 c          113 g          172 t
ORIGIN      
        1 aaaataaagt gtgcagtcgt ttcattaccg tatatcttta gtagttctaa taaactatca
       61 aaaatcgctc atttcagctt attgatagaa agattgattg ccagtctgtt tagagccgta
      121 cagcagcaaa ctttcataac caggttgatc ctgaatttgc agtacgaaaa gttaaaaatg
      181 agcgatttct gtaagactcc ccattttgcc aggcttatga ttcgcagatc attcaagtgc
      241 aaaggagcga tgtatggatt gttcaaaatg caatggttat gcgacgatgt tattaaatat
      301 ggtgcagggt tctgacccgg ttaatttact ggaattacat ggtttccttg aacatttcgc
      361 atattatgtt tcatttggca aatttaatgc tggccaccag cgctataatg catttaaaaa
      421 attcgtcagt gagatttcgg agatcagcgc aaatgatatc aatatgacga ttaaaaccgg
      481 gcaatccaga catgaaaatg tgatctctat aaatatgaat gatgcaattc ctcgtgatga
      541 gaaagggatc accgttaga
//
LOCUS       DAZGXS010000473          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGXS010000473 DAZGXS010000000
VERSION     DAZGXS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>558
                     /locus_tag="ACHKG5_005279"
     CDS             <1..>558
                     /locus_tag="ACHKG5_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0871145.1"
                     /translation="HAVSGSLHGYYVCPMRRLHRCDRPSIKRDLVDYNIINELLFNCS
                     KIQPVENKKDANETLELKIIELQMKINNLIVALSVAPEVTAIAEKIRLLDKELRRALV
                     SLKTLKSKAVSSLGDFHAIDLTSKNGRELCRTLAYKTFEKIIINTDNKTCDIYFMNGI
                     VFKHYPLMKTISAQQAISTLKYMVDG"
BASE COUNT          198 a           79 c          107 g          174 t
ORIGIN      
        1 tcatgcggta tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa gattacatcg
       61 atgtgacagg ccatcaataa agagagattt ggttgattat aatatcatta atgaattgct
      121 ttttaattgt agcaaaattc aaccagttga aaacaagaaa gatgctaatg aaactttaga
      181 gttaaaaatt attgagcttc agatgaaaat taataattta atcgttgcat tgtctgtcgc
      241 gcctgaagtt accgctatag cagagaaaat aagactatta gataaggaat tacgaagagc
      301 tttggtatca ttgaaaactt tgaagagtaa agcggtgagt tcacttggtg attttcatgc
      361 tattgactta accagtaaaa atgggcgaga gctatgtcgt acacttgcct ataaaacatt
      421 cgaaaaaatc ataatcaata cagataataa aacctgtgat atctatttta tgaatggcat
      481 tgtttttaaa cactatcctt taatgaaaac aatatccgcc cagcaggcga taagtactct
      541 caaatatatg gttgatgg
//
LOCUS       DAZGXS010000474          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGXS010000474 DAZGXS010000000
VERSION     DAZGXS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            263..>555
                     /locus_tag="ACHKG5_005280"
     CDS             263..>555
                     /locus_tag="ACHKG5_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871146.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKA"
BASE COUNT          160 a          107 c          173 g          115 t
ORIGIN      
        1 gtcgtggtta tcgggcggca gaggtaaaaa gaataaaaaa atcccgcaga gttgcgggga
       61 cagacaaaga ttaacgttaa ggagttattt ttgtttctgg tgctcgggca aaaaaaacat
      121 taacgcagag aaattattag caccacagtc agtttgtgaa aatgtgaaga tattcagaag
      181 ttttattcag tcatgataca ggcatcctcc gggatgcctg ttgtttttgt gcgtaacagt
      241 tatcacagta aagggtgaga caatgggcaa aggtggcggc aaggcgcaca cgccggtaga
      301 ggcaaaggac aatcttaagt ccacgcagat gatgagcgtg attgatgcca ttggtgaagg
      361 gccgattgaa ggtccggtga aggggctgca gagtatcctg gtgaacaaaa ccccgctgac
      421 ggacacggac ggtaatcctg tgatacatgg tgtgacagcg gtctggcgcg ccggggagca
      481 ggagcagaca ccacctgaag gctttgagtc ctccggggcg gaaaccgcac tgggcgtgga
      541 agtgacgaag gcaaa
//
LOCUS       DAZGXS010000475          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGXS010000475 DAZGXS010000000
VERSION     DAZGXS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>549
                     /gene="espJ"
                     /locus_tag="ACHKG5_005281"
     CDS             <1..>549
                     /gene="espJ"
                     /locus_tag="ACHKG5_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFP0871147.1"
                     /translation="KLLNRKITPENTPPMLLSYRNADLTQEKNITERVLSIFKIKRDF
                     VAVRIQNNQFTDLKNKKIQGHQNTVASVMDWYNPQKNALGITMGTPRKSADIAKEEHR
                     NALNFMIMEKNTFHEKILNNNDNLQKSYSKTEDSSWVAASVGSLLDKGAKVYPDTSCS
                     LRLGEPFIFTLPESVRVDVDIY"
BASE COUNT          223 a           96 c           93 g          137 t
ORIGIN      
        1 ggaagttgtt aaatagaaaa ataactccag aaaatactcc tccaatgttg ttatcataca
       61 gaaatgcaga tttaacgcaa gaaaaaaaca ttacagaaag agttttatcc atatttaaaa
      121 taaaacgaga ttttgttgct gtaagaatcc aaaacaatca gtttactgat ttgaaaaaca
      181 aaaaaattca aggccatcaa aacacagttg ccagtgtcat ggattggtat aatccccaaa
      241 aaaatgcttt gggtataact atgggtacac caagaaaatc ggccgatata gcaaaagaag
      301 agcacagaaa tgcactaaat ttcatgataa tggaaaaaaa cacatttcat gaaaaaatct
      361 taaacaacaa tgacaatttg caaaaatcat acagcaaaac agaagacagt agttgggtcg
      421 cagcttctgt cggctctctt ctagataaag gagcaaaagt atatcccgat acatcatgct
      481 ctctgagact gggagaacca ttcatcttta cgctgccaga atcagttaga gtcgatgttg
      541 acatatatc
//
LOCUS       DAZGXS010000476          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGXS010000476 DAZGXS010000000
VERSION     DAZGXS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            70..345
                     /locus_tag="ACHKG5_005282"
     CDS             70..345
                     /locus_tag="ACHKG5_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP0871148.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(425..>537)
                     /locus_tag="ACHKG5_005283"
     CDS             complement(425..>537)
                     /locus_tag="ACHKG5_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFP0871149.1"
                     /translation="YFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT          216 a          115 c           82 g          124 t
ORIGIN      
        1 tattcttaat gataccaact tataacggaa tagcataaaa acacttttca tggagcaaag
       61 gagaaaacaa tgccattttc aatcaaaaac agattttcaa gttcacaagt acattacccg
      121 gaaatatccg gtcccataaa agacaagcca gcgtcaaaga actgcatact tacatcaaca
      181 acatgtaatg tagatagcta tacagtgtac caaaaaaaag cctgtagttt tgacatgcgc
      241 ccacccggcg caggagaaag aaccccaaaa ctaaaactct cagttactga gatgacatgg
      301 ctatctaaaa ctatagaaac agagatacac aacacaaaag aatagcaacc acttaccaga
      361 gaacacaaaa agccacagac ccgaaacacc tgactgcaag caaccacctc cacaggaggt
      421 ggttttacca tgaacttcct gtgtactgac ttgtgttcat aataatattt ttgtgtttaa
      481 actcaataaa gtcacaaaaa tgattgtaat catgcaatgt agtaaaatta aaatatt
//
LOCUS       DAZGXS010000477          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGXS010000477 DAZGXS010000000
VERSION     DAZGXS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(36..>535)
                     /locus_tag="ACHKG5_005284"
     CDS             complement(36..>535)
                     /locus_tag="ACHKG5_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0871150.1"
                     /translation="TEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIQAAAANFH
                     FATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSP
                     AQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRDGGML
                     SGSGR"
BASE COUNT          110 a          197 c          115 g          113 t
ORIGIN      
        1 ctccatatcc ggcttcactt tccagcgaaa ggttttcatc gtccgctccc tgataacata
       61 ccgccatcac gcaactgcag ccggagttca tcctgcgccc ccttgcgggc catgtcatac
      121 accgccttca tcagctgcgg ccccgcctgt ccgttggtgc cgtcgttctg aatcaccacg
      181 tgattgttct gattaaaatt aataccttcc gcccgccgca tctgcgccgg acttccggca
      241 ccacccacat aaccaccttc cgcatagccg cgcatcagac ggtaaagatt ccccacacct
      301 atccggctgg ttgcctcttt cgtgaaaaca aactccccgc ggtgaactat ccccgcaggc
      361 tcatatttgc cgcccgtccc cgtaaatcct ccggtcgcga aatggaagtt cgccgccgca
      421 gcctgaatgg ccgtccccga ggaagcagat gcaccaccac cgaaagcacc gccaatggcg
      481 ctgccgatac gcccgacaat gcccaccatg gcctgtttaa gcaggatttc tgtca
//
LOCUS       DAZGXS010000478          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGXS010000478 DAZGXS010000000
VERSION     DAZGXS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..504
                     /locus_tag="ACHKG5_005285"
     CDS             <1..504
                     /locus_tag="ACHKG5_005285"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0871151.1"
                     /translation="IVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINT
                     YDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAE
                     QTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKED
                     DIISSCR"
BASE COUNT          174 a           84 c           94 g          181 t
ORIGIN      
        1 atcgtatttc atttgatggg tagtcatggt cctacctact atctccgtta tcctgagtca
       61 cataaatatt ttaaaccaac atgtgatcgt agtgatatcg aaaattgtac gcatgagcaa
      121 ttgattaata cctatgataa tacaattcgc tatactgatt atataatatc aaaattaatt
      181 gacaagctta tcgaatataa agatgagtat gatactgttt tattatatgt ctctgaccat
      241 ggagaatctc tgggagaaaa tggattatac ttacatggaa caccatataa tgttgctcca
      301 gctgagcaaa cgcatgtgcc tcttattacc tggatgtctc cggggttcgt ttcctctaaa
      361 aaaattgatt tgaattgttt gtcagaacat gctctaaaca gaacagtaag tcatgataat
      421 atattttcat cgttactggg gttatggaat gtaaatactt cagtttataa taaagaagat
      481 gatattatat cctcctgcag atagatactg acatttttct gaggataaga ggt
//
LOCUS       DAZGXS010000479          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGXS010000479 DAZGXS010000000
VERSION     DAZGXS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>530
                     /locus_tag="ACHKG5_005286"
     CDS             <1..>530
                     /locus_tag="ACHKG5_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP0871152.1"
                     /translation="CHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDL
                     ISGWEKRQVMKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQV
                     TTDTDTDKELNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRP
                     SLDFRQRAALWGMALP"
BASE COUNT          141 a          122 c          164 g          103 t
ORIGIN      
        1 atgtcacggc gagtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
       61 gcttgatgtg acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct
      121 ggatggcgat cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa
      181 tgtttcgcaa accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag
      241 actgcgggag cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg
      301 acaagtcacg acagatacag atacagataa agaattaaac cccacacata acgcgcgcat
      361 gcgcgagagt gctccagcca gtgagtcgaa tggcgcgccg ttgcagacag cagaacctga
      421 atacccggat ggcctgagcg aaccgatcgg gaaatttccg atgactggtg tctggcggcc
      481 gtcgctggat tttcgacagc gggctgcatt gtggggtatg gctctgcctg
//
LOCUS       DAZGXS010000480          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGXS010000480 DAZGXS010000000
VERSION     DAZGXS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>501
                     /gene="wzx"
                     /locus_tag="ACHKG5_005287"
     CDS             <1..>501
                     /gene="wzx"
                     /locus_tag="ACHKG5_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP0871153.1"
                     /translation="ILQLGTLATWSGDNFIISITLGVTYVAVFSITQRLFQISTVPLT
                     IYNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFGSEVVNIWTEG
                     KIQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILIAIPAKYIIVS
                     HFGLTV"
BASE COUNT          149 a           80 c           91 g          181 t
ORIGIN      
        1 ttattttaca gttaggcact ctggcaacat ggagtggtga taactttata atatctataa
       61 cattgggtgt tacttatgtt gctgttttta gcattacaca gagattattt caaatatcta
      121 cggtccctct tacgatttat aacatcccgt tatgggctgc ttatgcagat gctcatgcac
      181 gcaatgatac tcaatttata aaaaagacgc tcagaacatc attgaaaata gtgggtattt
      241 catcattctt attggccttc atattagtag tgttcggtag tgaagtcgtt aatatttgga
      301 cagaaggaaa gattcaggta cctcgaacat tcataatagc ttatgcttta tggtctgtta
      361 ttgatgcttt ttcgaataca tttgcaagct ttttaaatgg tttgaacata gttaaacaac
      421 aaatgcttgc tgttgtaaca ttgatattga tcgcaattcc agcaaaatac atcatagtta
      481 gccattttgg gttaactgtt a
//
LOCUS       DAZGXS010000481          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGXS010000481 DAZGXS010000000
VERSION     DAZGXS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..421)
                     /locus_tag="ACHKG5_005288"
     CDS             complement(<1..421)
                     /locus_tag="ACHKG5_005288"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP0871154.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSALPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVT"
BASE COUNT          118 a          164 c          134 g           83 t
ORIGIN      
        1 cggtgacggc agacacctcc atcagctccc agcgcagaaa atgcagtccg cctccgtcca
       61 gaaaggtgta ttcatgagga cggaagccca cggacagccc cctgaccagc ccggtcttaa
      121 tggccgccca ggcctcatcc agcctggcag ccagttgcga tggcatatcc ggcacgggct
      181 tcgccagtgt tgccgtgatt tccagccctt cgctgacccg gcgcaccgta cactgcccca
      241 cagggcggga atggtcatgc tgccagagaa acgggagcgc actgccaaac tccgcgccct
      301 ccggctccag gatgtcacca tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca
      361 cccgctcatc ctcactgaag gatttcaccg tcagcaggga gcaggcccgt ttaagagtca
      421 catcagcctc ctgaaaataa aaaaaccgcc gcagcggtcc atgatggtta cagggtgaac
      481 agggttatat gaaaaaaac
//
LOCUS       DAZGXS010000482          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGXS010000482 DAZGXS010000000
VERSION     DAZGXS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..268
                     /locus_tag="ACHKG5_005289"
     CDS             <1..268
                     /locus_tag="ACHKG5_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0871155.1"
                     /translation="KNPEKTLTVNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTT
                     DTFSKANTQSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
     gene            274..>497
                     /locus_tag="ACHKG5_005290"
     CDS             274..>497
                     /locus_tag="ACHKG5_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0871156.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTL"
BASE COUNT          131 a          106 c          163 g           97 t
ORIGIN      
        1 gaaaaatccg gagaagacac ttaccgtgaa taccggtgcg ctgctgacaa tgtcagtcag
       61 tgtcaacggg ggaacgccgc cttataaaca cgcctggaag aaggatggtc agccggtaga
      121 gggacagact actgacactt tcagtaaagc caatacgcag tcaggtgata agggggctta
      181 tacctgcgag gtaacggatt ctgcagaaca gccgcagagc attacctctg atgcgtgtac
      241 agtaacggtt aatggtgcgg gcggataagg cttatggcaa aagatctgaa aacactggcg
      301 ctggccagac tgtcggggtt ccgtcataaa acggtgaagg tgccggaatg gagaaatgtc
      361 agcgtggtgc tgcgggagcc ttcggcagag gcctggtatc tgtggcagga agtgctcaat
      421 ggtgatggag aggatgacga taccctgtcg gtggtggcga aaacccgccg taacctggaa
      481 gcggatgtga cgctgtt
//
LOCUS       DAZGXS010000483          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGXS010000483 DAZGXS010000000
VERSION     DAZGXS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            59..>491
                     /locus_tag="ACHKG5_005291"
     CDS             59..>491
                     /locus_tag="ACHKG5_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP0871157.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPG"
BASE COUNT          190 a          100 c           87 g          114 t
ORIGIN      
        1 cattcatttg tccagcactc catactttca gccgttggat tggtatataa taaaagccat
       61 gctctggact gaaaacgatg cggaaaatac ttctcagtgg aatggttatc cattacaaat
      121 tggtcgtttt cggaaagata aagcgatgcc agctttaatc tctggagaaa aatctacagc
      181 ccttgttacc ccaccacaat ggagaaacaa agcattcaat gggttaaaag atccagaaag
      241 aaattactgg gcaaaagaac aaataactgg ctctccagaa gagaatatca aagcagcaat
      301 tacttactta atgatgaaac tttctaatac aaaggaagaa tcaactatcg atcaatatga
      361 ctcaactcta tatagcgcca ttgtccaaaa aggcgatttg gctgacaaca tcagaaaaga
      421 gagaaaaacg accataccta acttaacgaa aaacaatcct ggaaaaaatc tcgataaaat
      481 acacccggga g
//
LOCUS       DAZGXS010000484          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGXS010000484 DAZGXS010000000
VERSION     DAZGXS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>479
                     /locus_tag="ACHKG5_005292"
     CDS             <1..>479
                     /locus_tag="ACHKG5_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP0871158.1"
                     /translation="RISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLGREHRMDRRA
                     VALERQLNGGVDFLSSVNNYFQSVMAEHRENKTGNKILMEKINSCVFGTDSNHFSCPE
                     SFLTCPITLDKPENGVFMRNSQGAEICSLYDKDALVQLVETGGAHPLSREPITESMI"
BASE COUNT          149 a           74 c          118 g          138 t
ORIGIN      
        1 agaatatcca ttggtagtgg ccaatattct gttcactatg tccaactact ggatggattt
       61 tcagttgaac cggtgagagg aggcttactg gataggctat tggggcgtga gcatcgaatg
      121 gatagaaggg ctgtggctct ggaaaggcaa ttaaatggag gtgtcgattt tttaagtagt
      181 gttaataact attttcagag tgtcatggca gaacacagag aaaataaaac aggtaataaa
      241 atattaatgg aaaaaataaa ttcttgtgta tttggaacgg attctaatca cttttcttgc
      301 ccggagtcat ttttgacatg cccgataacg ctggacaaac ctgagaatgg agtgttcatg
      361 agaaactcac aaggtgctga gatatgctct ctatatgata aggacgcgtt agtgcaactt
      421 gttgaaactg gtggagctca tcctctgagt cgagaaccta taacagaatc aatgattat
//
LOCUS       DAZGXS010000485          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGXS010000485 DAZGXS010000000
VERSION     DAZGXS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>479
                     /gene="espR3"
                     /locus_tag="ACHKG5_005293"
     CDS             <1..>479
                     /gene="espR3"
                     /locus_tag="ACHKG5_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFP0871159.1"
                     /translation="AYDKTGSITIEKSQGEGTLPIRHKLEFISTNIAELLDKLTKITD
                     ARLCKGFSDWASSVKEGASNDLKENVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLP
                     EWIEMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITLISVNNNLISYIDS"
BASE COUNT          151 a           85 c          104 g          139 t
ORIGIN      
        1 ggcttatgat aagacgggat ctatcacgat agaaaaaagt cagggggagg ggactttacc
       61 cattcggcat aaattagagt ttatatcgac caatattgca gagttgctgg acaagttaac
      121 caaaattaca gatgccaggc tttgcaaagg tttcagtgac tgggctagtt cagtcaaaga
      181 aggcgcatcg aatgacttga aagaaaatgt ggatagagca ttggtgagaa tgtttaaatg
      241 tgttaagctt cacagtaatg aacttaactt atcaagcctt tctttgggtt ctgtgccgcc
      301 tcttcctgag tggattgaaa tgcttagcct tgtttataat gaacttgatt caatacaggt
      361 gcccgaatcg tgcaaagaat tagaactcga tttcaataac cttacagaat ttccacaagt
      421 acctgatgga attaccctga tctccgtaaa taataacctg atatcgtata ttgactcat
//
LOCUS       DAZGXS010000486          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGXS010000486 DAZGXS010000000
VERSION     DAZGXS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>468)
                     /locus_tag="ACHKG5_005294"
     CDS             complement(<1..>468)
                     /locus_tag="ACHKG5_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871160.1"
                     /translation="LSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAMQDFLNDIN
                     FENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSRKGNPFIGF
                     VVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVILENKGPT"
BASE COUNT          151 a           96 c           69 g          152 t
ORIGIN      
        1 aacgtcggac ctttattctc aagaattaca atctggaaat tatagtccct tttgattaat
       61 gcgtgataaa atgaatcact gagactgacg ctattaacat ccgatagaga accccctcta
      121 tctttatcaa agtgcgtcac taatggacta tcaagcacaa caaatccaat gaatggattc
      181 ccttttcttg ataaaaattc cgcaaagcaa atggtaaata tagcatgcat tactgcacgg
      241 gtacctttac caaacattcc tcggcgtttc ccattgatat taaagtcata tgtatcttcg
      301 tcaaatcgca ctttgttatc tttagaattt tcaaaattta tatcattcaa aaagtcttgc
      361 atcgctattt ctatatcata caaatcagct actgttaagg agtcaaagtc ctcggcggag
      421 tatttaagtt cgtcaacatc atcttcttta tcttgtaatg aagataga
//
LOCUS       DAZGXS010000487          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGXS010000487 DAZGXS010000000
VERSION     DAZGXS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            72..>464
                     /locus_tag="ACHKG5_005295"
     CDS             72..>464
                     /locus_tag="ACHKG5_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP0871161.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          136 a          122 c          105 g          101 t
ORIGIN      
        1 agcctgtaca tagatttgtg taattgcctg attttgatat gttcaatcca acatcaaaag
       61 caggttaatt tatggacgaa aaacagttgc aggctctggc taacgaactg gccaaaaatc
      121 tcaaaacccc tgaagatctc agccagttcg atcgcctgct gaagaaaatc agcgttgagg
      181 cagctctcaa cgctgaaatg tcccaccatc tgggctacga taaaaaccag cctaaaccgg
      241 gtgccaactc ccgcaatggc tattccacaa agaccgttat caccggtgat ggccacctgg
      301 aactacgcac tccgcgcgat cgtgatggct cttttgaacc gcaacttgtg aagaaaaacc
      361 agacccggat caccgggatg gataaccaga tcctgtcgtt gtacgccaaa ggcctgacaa
      421 cccgcgaaat agcggctgcg tttaaagagc tgtatgacgc tgat
//
LOCUS       DAZGXS010000488          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGXS010000488 DAZGXS010000000
VERSION     DAZGXS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>464
                     /locus_tag="ACHKG5_005296"
     CDS             <1..>464
                     /locus_tag="ACHKG5_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871162.1"
                     /translation="FTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQPINS
                     TFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTPL
                     TRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRF"
BASE COUNT          174 a          104 c           81 g          105 t
ORIGIN      
        1 tttaccccag gtatgataaa tacatatcac ggcgatattt ataaccgcac aacagatacc
       61 gacaatgtta aaacaccaga cacaccgatg tggccttgtg ataatcggga agaacaacaa
      121 cccattaatt ccacattctc tggagaagga tacaaccctg aacaatatga tttagcgcgg
      181 caacacctcc cacaaattta tgcgtgccgc acgaacacca aatatacaga tgcagactac
      241 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaatcgat aagttcgaca
      301 ccacttacgc ggcaggcaca ggaaatactt aatcaaatta ataacattag atacgaaaag
      361 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      421 ataactaaaa ttagcgtaga agagggaata ccagtaagat tcag
//
LOCUS       DAZGXS010000489          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGXS010000489 DAZGXS010000000
VERSION     DAZGXS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            357..462
                     /locus_tag="ACHKG5_005297"
                     /pseudo
     CDS             357..462
                     /locus_tag="ACHKG5_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          125 a          113 c           84 g          140 t
ORIGIN      
        1 ttaattatgt aaggagtatt tgatcatgta taagcaataa aaaacagctt caggtaatga
       61 ggaatatctc aatttttaaa cataaaatgc gaattattta gtacaaaaag tgaacttttg
      121 atgatctttc cctaatctca cataggatca gatctcgtct gatcgttccc cctgcgctca
      181 ctaccatggc tctttaccac agcagccggg gttgtttggg accgacttcc gacagcagat
      241 cccggtgggc ctgccaccat ctcttcacaa gcttatgctt attacatgca aaacgtcccg
      301 ttttgcatct ttgtgcctac ggcacaccga tgatctttcc ttgtgatctt tccctgaatc
      361 tgcccctgag aacctgaatc aaacgtctga ctcgcagaac catctgtttc tcgcgctctg
      421 tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa at
//
LOCUS       DAZGXS010000490          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGXS010000490 DAZGXS010000000
VERSION     DAZGXS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..341)
                     /locus_tag="ACHKG5_005298"
     CDS             complement(<1..341)
                     /locus_tag="ACHKG5_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0871163.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVL"
BASE COUNT          107 a          133 c          114 g           94 t
ORIGIN      
        1 ttgagtacca gtgcatcatc gcgtagctgt aagcggatat cagaaggcgc tttccagccg
       61 gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt agctgctgta ggtgcgggag
      121 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
      181 cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc ccgggcacac cgagcacgcc
      241 acgccagttg gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac
      301 tgagtcatat ctccctgacg cgccgctggt tttccgctca tataaagtgc ccttttagtt
      361 attgttcata tccctgtgtt agcaacaaaa aaaataaaat gacacacaag accagctaat
      421 gtatttatat atttttcaca taaactta
//
LOCUS       DAZGXS010000491          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGXS010000491 DAZGXS010000000
VERSION     DAZGXS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(301..443)
                     /locus_tag="ACHKG5_005299"
                     /pseudo
     CDS             complement(301..443)
                     /locus_tag="ACHKG5_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
BASE COUNT          108 a          142 c           72 g          121 t
ORIGIN      
        1 taagaaactc taactctctc ccctcccagg agaaatccac caatctgccc ttccattacc
       61 tgctgacgca tctccgccat ctcattcgcg aatgaagtcg atgatatata tgatactaat
      121 ccaggcatta ccatattcct tcatacagat aaatatttcc tctgggcttc ataacaaaca
      181 tctccctgac atgacaacaa aaaccggagc cggactccgg tttttgtgaa tccgtcggct
      241 atttcatccc gccaatattt ccccgtcagc acgccagatt tgcagcggcc tcaccactca
      301 ctgtatatgc tttttatccg catccagtat caccacctgc gtgattaccc tgtcctgctc
      361 cgggataata ccattctcat cggactccag aatgtctgcc ggtcccagtc gcagctgtgc
      421 tgtaagtaac tccccgtttt cac
//
LOCUS       DAZGXS010000492          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGXS010000492 DAZGXS010000000
VERSION     DAZGXS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(246..>441)
                     /locus_tag="ACHKG5_005300"
     CDS             complement(246..>441)
                     /locus_tag="ACHKG5_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP0871164.1"
                     /translation="TKNCILDSTTCNVDGFTVFNRRSCSFDMRPPGSADRTPQLRLSI
                     SEVAWMSKIIETETNNTNKS"
BASE COUNT          130 a           81 c           85 g          145 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt a
//
LOCUS       DAZGXS010000493          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGXS010000493 DAZGXS010000000
VERSION     DAZGXS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            91..309
                     /locus_tag="ACHKG5_005301"
     CDS             91..309
                     /locus_tag="ACHKG5_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871165.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          140 a           54 c           91 g          155 t
ORIGIN      
        1 ttctctaaat tatgaattaa atatccataa gcattattgc ctgggaactg gacatataat
       61 tactcttctg tgctaaagga acgttctgaa atgtttaagt atgcagagat gttgatggcg
      121 gaagaagatg ataaagtatg tgaacttatt tttaaacaac aagataattt gattttaaaa
      181 ataaatgtta gatttcccag aataattata aagaaatacc gaacattaca aggtaaagtc
      241 gtatggctat ggatattgcc atttgttatt gaatggttaa ttgtgtgtac tttgggtgta
      301 aacagttagc ttggtaaaag taaatatcct gattgttgtt tgttaaggag tgagatgcct
      361 gtgcgtctgg tgtctattat attcccaata ttattggcgc tattcttgct ggcgtaggca
      421 tcgccatact atgaatattg
//
LOCUS       DAZGXS010000494          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGXS010000494 DAZGXS010000000
VERSION     DAZGXS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>438
                     /locus_tag="ACHKG5_005302"
     CDS             <1..>438
                     /locus_tag="ACHKG5_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0871166.1"
                     /translation="FVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADE
                     MAEMKTSITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDG
                     IPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFV"
BASE COUNT          129 a           95 c          133 g           81 t
ORIGIN      
        1 tttgtggaag tcagcgggca gcccagcaat gatggtgaag ggtatctgga atttttccgg
       61 gaaaaaatag gaaaactgca tctggctcag gggttgtggg aactgataga taacagccag
      121 cttgcggatg agatggcgga gatgaagacc agcatcacgg aaacccgcaa tgaaatcaca
      181 cagacggtca gtaaaacgct ggaggaccag agcgccacca tacagcagat acagcgcgtg
      241 cagaaggaca caaatgatga cctggctgcg ctgtacatgc tgaaggttca aaaaacgaaa
      301 gacggcattc cctatgtggc cgggattggt gcagggattg aggatactga tggccagcca
      361 ctgagcaaca tactgctgct ggctgaccgt atcgcgatga taaatccgga gagcggcaac
      421 agcacgccgt tatttgtg
//
LOCUS       DAZGXS010000495          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGXS010000495 DAZGXS010000000
VERSION     DAZGXS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHKG5_005303"
     CDS             complement(<1..203)
                     /locus_tag="ACHKG5_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0871167.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>437)
                     /locus_tag="ACHKG5_005304"
     CDS             complement(355..>437)
                     /locus_tag="ACHKG5_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0871168.1"
                     /translation="IDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          118 a          123 c           88 g          108 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatgg
//
LOCUS       DAZGXS010000496          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGXS010000496 DAZGXS010000000
VERSION     DAZGXS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>437
                     /locus_tag="ACHKG5_005305"
     CDS             <1..>437
                     /locus_tag="ACHKG5_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871169.1"
                     /translation="THEYKIDHQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKK
                     IADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQISSSGYVSADKLHKLYKTN
                     LEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQ"
BASE COUNT          171 a           78 c           87 g          101 t
ORIGIN      
        1 tacacatgaa tataaaatag atcatcaaca gttgaaaaat cacataaggc aacttgagga
       61 gtgggccctt cttgaaaagt tttatgataa caacgcttat tattctaaac gtacaaaaca
      121 attagagaag aaaattgccg atgcggggtt tataacaagg aaggttaaag ccgagaactt
      181 taatgtaaaa gagtttaaag acgatatagc ctcgatgctt tgtgaacagc cccccatgcg
      241 tacacaccag atttcttcat ccggatacgt atctgccgat aaactgcaca aactctataa
      301 gacaaatttg gaagacgcgc agagagatct gaaaacaaca ccggttgaca tcgatggtgt
      361 tagtctaaaa tcaataactc gggaaaaaat tgcggaagaa attgcgaaaa aaactgcgga
      421 aataattgcg aaacaaa
//
LOCUS       DAZGXS010000497          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGXS010000497 DAZGXS010000000
VERSION     DAZGXS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>435)
                     /locus_tag="ACHKG5_005306"
     CDS             complement(<1..>435)
                     /locus_tag="ACHKG5_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0871170.1"
                     /translation="VFLCLAVILGIYFYFKFKKKGTEETVVEGDITVGLEPYNQDDDI
                     SLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYGIRIPGIVIRE
                     GEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLI"
BASE COUNT          152 a           94 c           56 g          133 t
ORIGIN      
        1 ttgatcaaag tcgaaaccac ttcatctgaa tattcaacca acaagacaag atcatgataa
       61 attttaaatt gtgaagctct gacttcattt agcattaata tgacatcttc atcattaaga
      121 ccttccccct cacgaatgac aataccggga atgcggatac catactcaat aaaaaactga
      181 ctacgaatcc tatcagcaag attgatttca gtgtattttt tagcctgtac actattcata
      241 atcaagacca agggtaccgt ttcggtaata acctgatcaa gtttattaat gatacctaat
      301 gagatatcat catcttgatt atagggttcc aacccaacgg taatatctcc ctcaacaacc
      361 gtttcttcag tccctttctt tttgaattta aagtaaaagt aaattcctaa gattactgcc
      421 aaacataaaa acact
//
LOCUS       DAZGXS010000498          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGXS010000498 DAZGXS010000000
VERSION     DAZGXS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>429
                     /locus_tag="ACHKG5_005307"
     CDS             <1..>429
                     /locus_tag="ACHKG5_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0871171.1"
                     /translation="IPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRF
                     GRYELTTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSDSGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPD"
BASE COUNT           95 a          154 c          118 g           62 t
ORIGIN      
        1 tataccggac agcctgcccg ccgtggaatg gctgacctat ggcagcggct ggctggcagg
       61 catgaagctc ggcgacacac cgctggtgga tttcacccgc gaccgcctgc accggaaaac
      121 gctgcgcaga ttcggccgtt atgaactcac caccgcttat acccctgccg ggcagttaca
      181 gagccagcac ctgaacagcc tgcagtatga ccgcgattac acctggaacg acaacggcga
      241 actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagcg actccggcag
      301 gctgaccggc gttcacacca ccgcagcgaa tctggatatc cgcatcccgt atgccacgga
      361 cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg
      421 gccggataa
//
LOCUS       DAZGXS010000499          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGXS010000499 DAZGXS010000000
VERSION     DAZGXS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            77..>428
                     /locus_tag="ACHKG5_005308"
     CDS             77..>428
                     /locus_tag="ACHKG5_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0871172.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASA"
BASE COUNT          134 a           94 c           98 g          102 t
ORIGIN      
        1 caacccttga ttctgtatag ataaatttac accacctttg aaaacaatcc gctatgcttt
       61 gaaaaaggag aaagaaatga tgaaaaagtt tatcgccccc ttgttggctt tactggttag
      121 cggatgccag attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga
      181 tgttggtatg gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga
      241 ttcacaggcg gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg
      301 ccagaccgat tttacttatg cccgaggact ttccggtaaa agctttcctg ccagctgcaa
      361 caatgttgaa aatgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc
      421 cagcgcaa
//
LOCUS       DAZGXS010000500          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGXS010000500 DAZGXS010000000
VERSION     DAZGXS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>428
                     /locus_tag="ACHKG5_005309"
     CDS             <1..>428
                     /locus_tag="ACHKG5_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP0871173.1"
                     /translation="KREYKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPT
                     HIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRY
                     DYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYI"
BASE COUNT          154 a           74 c           83 g          117 t
ORIGIN      
        1 ttaaaagaga atacaaggaa caagaaggaa atcctgaaat taagtctaaa cgcagagagc
       61 gccatcagga aattctttct gagcaattga aatctgatgt cagtaatagc cgtttgatga
      121 ttgccaaccc tactcacatt gcaataggga tatactttaa gccacatctg tcacctattc
      181 cattgatttc tgtaagagaa actaatgagg tagcattagc tgtaaggaaa tatgcaaagg
      241 aaatcgggat accaattatt acagataaaa aattagcacg aaaaatttat gctacccatc
      301 gtcgctacga ttatgttagc ttcgaaaata tagatgaaat attacgtctt ctgctgtggc
      361 ttgaagatgt ggagaatgct ggacaacctg ttccagatga acagctctct tcagaagata
      421 aatatatt
//
LOCUS       DAZGXS010000501          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGXS010000501 DAZGXS010000000
VERSION     DAZGXS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..95)
                     /locus_tag="ACHKG5_005310"
     CDS             complement(<1..95)
                     /locus_tag="ACHKG5_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871174.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEA"
BASE COUNT          124 a           97 c           91 g          113 t
ORIGIN      
        1 gcctcctcag cgaggttgcc tgaataccct gtcgtgccca tttttccatc ggacgcagtt
       61 gtctgctctc tcgaactact ggcaataaaa atcaaccaga cagcttgatg tccgttacat
      121 gcaggactaa tcacgattgg ttaccgttta caaaacttgc gtaacattgg aggagaacga
      181 ataatattct ctagctggta cgcgttccat agcgaaacgg gcaaggtcca acagcaagag
      241 ctgaaactca gggggattta tcagtcgatt tattcgccat ggatgaatca cgctgtacgg
      301 ttatggtagc tcttagcgac aaggcataat catcagtaca ccaatgtaac aaccgttgaa
      361 aaagactcat tattttatga accagccatc ccatttccag agtggtaaat atgatttgtt
      421 aaagt
//
LOCUS       DAZGXS010000502          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGXS010000502 DAZGXS010000000
VERSION     DAZGXS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..174)
                     /locus_tag="ACHKG5_005311"
     CDS             complement(<1..174)
                     /locus_tag="ACHKG5_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0871175.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGRQYQA"
     gene            complement(174..>421)
                     /locus_tag="ACHKG5_005312"
     CDS             complement(174..>421)
                     /locus_tag="ACHKG5_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0871176.1"
                     /translation="YSQRMAAGLNADLKTYRVTLSVTREEARHLEAFLAEHGGWKAFL
                     WKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT           85 a          150 c           90 g           96 t
ORIGIN      
        1 tgcctgatat tgccgcccct gccaggtaac cgcctcccct ttttcattca gctcattgca
       61 gaaaaaatac cgctcaccgc cctgcgccgt caggtcgatt tcccagagta ccacccgcgg
      121 tgactgctct gatttaaccg actcgttcag actttcttcg tgaatatcct gcatcagttc
      181 accacctgct taaactccgc gctgaactca acgcgcaaca tcccgacccg cgcagaccac
      241 ccggcacagg tcacctttat ctgccggtat gcatagggtg gcttccacaa aaatgccttc
      301 cagcctccgt gctctgccag gaacgcttcc agatgccggg cctcctcccg ggtcacggaa
      361 agcgtcacac ggtatgtttt caggtcagca ttcagccctg ccgccatacg ctgtgagtac
      421 c
//
LOCUS       DAZGXS010000503          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGXS010000503 DAZGXS010000000
VERSION     DAZGXS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            44..409
                     /locus_tag="ACHKG5_005313"
     CDS             44..409
                     /locus_tag="ACHKG5_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0871177.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCHCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT           85 a          102 c          132 g          101 t
ORIGIN      
        1 attttctttc ctctggtgac aagctatatg gcgaggattt tacatggctg tgcttcgtac
       61 gttaccgggc agaatcaaaa ctctgaacac ccggcgggtg aatattctga agggtgaaca
      121 gcgtcgtgtc agtggcagtg cacgtgtttc cctcaagcgt catatctggc tcagggatgc
      181 cgggcagtgc tgtcactgtg gtcgtgtggt tgacctctgt gacagtgaac tcgatcaccg
      241 aattgcactt cagttcggtg gtggtaatga ggagacgaat ctctggacgc tctgtaccga
      301 atgccatcga caaaagtctg ctcgtgaagc ggcgggtggt atgccggacc cgacgctgcc
      361 ggaggtgtcc ggaggtcatg gcagggcaga cgatatcatc ggactgtgac ccgccccggg
//
LOCUS       DAZGXS010000504          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGXS010000504 DAZGXS010000000
VERSION     DAZGXS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>420
                     /locus_tag="ACHKG5_005314"
     CDS             <1..>420
                     /locus_tag="ACHKG5_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFP0871178.1"
                     /translation="QCEMKNFKIETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQ
                     DGVCKEASVIASSLLSCNIAKLNGVGMDFIETSFYGKTRGGFRDSNNFYDCDFRYVNM
                     TCKRDGIREDLRDYDPRKYFGESENKNKLNLDKALLNF"
BASE COUNT          156 a           46 c           84 g          134 t
ORIGIN      
        1 caatgcgaaa tgaaaaattt caagatagag acatcaaaaa tatactctat aaatcaagat
       61 ccccaaagag gggataagaa agtcggtgca tatctgttta tgcagtcatt tgtatatgca
      121 agtaatcttc aagatggagt gtgtaaagaa gcaagtgtta ttgcaagcag tttattatct
      181 tgcaatatag caaagcttaa tggtgttgga atggatttca tagaaacttc gttttatgga
      241 aaaactcgcg gtggttttag agatagtaat aatttttatg attgtgattt tcgttatgtt
      301 aatatgacat gcaaacgaga tggaattcgt gaagatttac gagattatga tccaagaaaa
      361 tatttcggag aaagtgaaaa taaaaataaa cttaatctgg ataaagctct tttaaatttt
//
LOCUS       DAZGXS010000505          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGXS010000505 DAZGXS010000000
VERSION     DAZGXS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..75)
                     /locus_tag="ACHKG5_005315"
     CDS             complement(<1..75)
                     /locus_tag="ACHKG5_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871179.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKK"
     gene            93..284
                     /gene="yfgG"
                     /locus_tag="ACHKG5_005316"
     CDS             93..284
                     /gene="yfgG"
                     /locus_tag="ACHKG5_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0871180.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          101 a          108 c           81 g          129 t
ORIGIN      
        1 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
       61 cacggcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
      121 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
      181 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
      241 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac
      301 tttcacagaa cataacggcg cttcgctgtc ggatgctttt gctctttgga attatcaaag
      361 cggcagatat tctttcatct taaattatac gtctttgtcc cgactgatgt ttatcctgt
//
LOCUS       DAZGXS010000506          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGXS010000506 DAZGXS010000000
VERSION     DAZGXS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>418
                     /locus_tag="ACHKG5_005317"
     CDS             <1..>418
                     /locus_tag="ACHKG5_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0871181.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW"
BASE COUNT           90 a          109 c          131 g           88 t
ORIGIN      
        1 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
       61 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact
      121 gttgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
      181 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc
      241 gactgttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
      301 ccagcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
      361 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggat
//
LOCUS       DAZGXS010000507          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGXS010000507 DAZGXS010000000
VERSION     DAZGXS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            136..>417
                     /locus_tag="ACHKG5_005318"
     CDS             136..>417
                     /locus_tag="ACHKG5_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871182.1"
                     /translation="MEYRFSWVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILVINADSDLIKNDVLLAENIPTADYQFNTF"
BASE COUNT          134 a           55 c           81 g          147 t
ORIGIN      
        1 ttgtgggtat aaaatataat ctttttgaac aagtgaattt gagctaagaa taaatcaagt
       61 tgtttcagtg tttcgttaat taatatgagt ataaaaggtg ttgtgctttt ataataataa
      121 agcgcgagga gttcggtgga gtatcgtttt tcttgggtga tttttaatat gataaacaat
      181 atatctgatc aagcgtcatc atttccatgc acacaattga atcaaagtga taatttcctc
      241 gattcactga gggaattttt cgcgatatta aattccagtc gtaagggtga gttgtctaca
      301 tgggatacca tctacttgca tctgatatta gtaataaatg ctgacagtga tctcataaaa
      361 aatgatgtgt tgctcgcgga aaatattcca acagcggatt atcaatttaa tacattt
//
LOCUS       DAZGXS010000508          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGXS010000508 DAZGXS010000000
VERSION     DAZGXS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(16..375)
                     /locus_tag="ACHKG5_005319"
     CDS             complement(16..375)
                     /locus_tag="ACHKG5_005319"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0871183.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          136 a           93 c           66 g          122 t
ORIGIN      
        1 tttagatgtt gggcgttatg aaagtaataa aactccccat ataatgatag caactaaaat
       61 cgtaatcaac actgcggctg aacccatatc ttttgctctg cctgataatt cgtgatgttc
      121 tgaaccaatt ctgtcaacaa ctgcttcaat tgcactgtta attatttcaa caataattac
      181 catcattgcc gaaccaatga gtaatactcg agtaacagag tcaacatcca gccaaaagga
      241 tattatcagt gctgacagaa ccagaatgca ctcctgacga aaggctgctt catagcgcca
      301 tgccgcacat aagcctttcc atgaataacc cgctgcttta ataattctgg taacaccagt
      361 ggtcgtatta gccataaata cctcttattt aattatatgt ctgaatatgc attataa
//
LOCUS       DAZGXS010000509          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGXS010000509 DAZGXS010000000
VERSION     DAZGXS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>415
                     /locus_tag="ACHKG5_005320"
     CDS             <1..>415
                     /locus_tag="ACHKG5_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0871184.1"
                     /translation="QDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLG
                     LGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAA
                     DDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSR"
BASE COUNT          105 a           95 c          134 g           81 t
ORIGIN      
        1 ttcaggatgt gcttgccggg ctggaagacc gtcgtgtggc gttaattcgt cagcaggcgg
       61 cagagcagaa taaggtgtac cagtccatgc tggttatgaa cggtcagcat acggaattca
      121 accgtctgct ggggctgggg aatgaactgc ttcagcagcg tcagggactg gtgaatgtac
      181 cgttacggct gccgcaggcc accctggatg ataaacagca gagtgccctg acaaaaacag
      241 agcgtgagct ggccctgtcc agactgaaag gggaagaaaa agagcgtgtc cgactggggt
      301 atgcggcgga tgacctcggt tttgtgggtg atccgtatca ggaggcgaga caacgttata
      361 tcagtaatgc cctggaagcc tggcgcaata acgaggcgaa taaacccaaa tcccg
//
LOCUS       DAZGXS010000510          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGXS010000510 DAZGXS010000000
VERSION     DAZGXS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>414
                     /gene="wzx"
                     /locus_tag="ACHKG5_005321"
     CDS             <1..>414
                     /gene="wzx"
                     /locus_tag="ACHKG5_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP0871185.1"
                     /translation="ISKILSLLSLILTVSLTLPYLGQERFGVWMTITSLGAALTFLDL
                     GIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAICYITSGMIDWQLVIK
                     GINENVYAELQHSIKVFVIIFGLGIYSNGVQKVYMG"
BASE COUNT          128 a           58 c           85 g          143 t
ORIGIN      
        1 attagtaaaa tactctcact actttctctt atattaactg taagtttaac tttaccttat
       61 ttaggacaag agagatttgg tgtatggatg actattacca gtcttggtgc tgctctgaca
      121 tttttggact taggtatagg aaatgcatta acaaacagga tcgcacattc atttgcgtgt
      181 ggcaaaaatt taaagatgag tcggcaaatt agtggtgggc tcactttgct ggctggatta
      241 tcgtttgtca taactgcaat atgctatatt acttctggca tgattgattg gcaactagta
      301 ataaaaggta taaacgagaa tgtgtatgca gagttacaac actcaattaa agtctttgta
      361 atcatatttg gacttggaat ttattcaaat ggtgtgcaaa aagtttatat ggga
//
LOCUS       DAZGXS010000511          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGXS010000511 DAZGXS010000000
VERSION     DAZGXS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..243
                     /locus_tag="ACHKG5_005322"
     CDS             <1..243
                     /locus_tag="ACHKG5_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP0871186.1"
                     /translation="AEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            240..>406
                     /locus_tag="ACHKG5_005323"
     CDS             240..>406
                     /locus_tag="ACHKG5_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0871187.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          113 a           95 c          129 g           69 t
ORIGIN      
        1 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
       61 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      121 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
      181 gcagcaacca acaagaaaac actggcagaa tacgcccgtg ccttatccgg agaggatgaa
      241 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
      301 aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacgacc acttccgtgt
      361 ctgacctgaa aaaatacatt gctgagctgg aagtgcagac cggcat
//
LOCUS       DAZGXS010000512          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGXS010000512 DAZGXS010000000
VERSION     DAZGXS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..70
                     /locus_tag="ACHKG5_005324"
     CDS             <1..70
                     /locus_tag="ACHKG5_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871188.1"
                     /translation="LMLVALFLSMAWMALTSAGLVG"
     gene            82..330
                     /locus_tag="ACHKG5_005325"
                     /pseudo
     CDS             82..330
                     /locus_tag="ACHKG5_005325"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            293..>403
                     /locus_tag="ACHKG5_005326"
     CDS             293..>403
                     /locus_tag="ACHKG5_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871189.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPP"
BASE COUNT           89 a          101 c          112 g          101 t
ORIGIN      
        1 actaatgctg gttgctcttt ttctttccat ggcctggatg gcgttgactt cggcagggct
       61 ggtggggtga gcataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt
      121 ggtgcgctta gtctggggct gaatcattac cgcgataacg ccataaccta caaagagcag
      181 cgcgataaaa aagtcagtga gctggagctg gcaaatgcaa ccattactga tatgcagcag
      241 cgccagcgtg atgttgctgc acttgatgcc agatactcga gggaattagc tgatgcgaga
      301 gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcgcatcaac
      361 gccaactgtc caggctcctt gcgtaaagcc cccatcacct ccg
//
LOCUS       DAZGXS010000513          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGXS010000513 DAZGXS010000000
VERSION     DAZGXS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            141..>393
                     /locus_tag="ACHKG5_005327"
     CDS             141..>393
                     /locus_tag="ACHKG5_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP0871190.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSRPAPPPPTSG"
BASE COUNT           96 a           78 c           96 g          123 t
ORIGIN      
        1 agttattgta cagtttggtc catgagaatg gtagtggctg ggtacgagga ttttaattta
       61 tattgattta aaaatcattt tattattcgt tttgatatat atgagagtta gccaagctta
      121 gatataaaga ggcataaatt atgcttaatg gaattagtaa cgctgcttct acactagggc
      181 ggcagcttgt aggtatcgca agtcgagtga gctctgcggg gggaactgga ttttctgtag
      241 cccctcaggc cgtgcgtctt actccggtga aagttcattc ccctttttct ccaggctcgt
      301 cgaatgttaa tgcgagaacg atttttaatg tgagcagcca ggtgacttca tttactccct
      361 ctcgtccggc accgccgcca ccgacaagtg gac
//
LOCUS       DAZGXS010000514          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGXS010000514 DAZGXS010000000
VERSION     DAZGXS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..338)
                     /gene="escQ"
                     /locus_tag="ACHKG5_005328"
     CDS             complement(<1..338)
                     /gene="escQ"
                     /locus_tag="ACHKG5_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP0871191.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDI"
     gene            complement(328..375)
                     /locus_tag="ACHKG5_005329"
     CDS             complement(328..375)
                     /locus_tag="ACHKG5_005329"
                     /inference="COORDINATES: protein motif:HMM:NF018255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFP0871192.1"
                     /translation="MFPDEAIEFTIEYEN"
BASE COUNT          119 a           89 c           69 g          107 t
ORIGIN      
        1 taaatatctt tatttagcat cgtaaattga acatcatcaa ctgacacatc accttccggt
       61 aaggcagtct gcattaccca taagatcatg agttttatcg tatcttccgg tatcagccgg
      121 ctttcgctat acgggaataa ctcttggcac cactgaatga actgttcttc tgatatccag
      181 aaacgcccct catagcccga gtatgtcaac gtgaaacgaa taccatgacc gctgacctca
      241 cgaaaatatg catggcaagc atgacctttg gacgtaatat agaggcgacc ggttccgata
      301 actggcaaca acggcaaata aaaatcgtta attttcatat tcaattgtga actcaatggc
      361 ttcatcagga aagatttctt ttaa
//
LOCUS       DAZGXS010000515          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGXS010000515 DAZGXS010000000
VERSION     DAZGXS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>384
                     /locus_tag="ACHKG5_005330"
     CDS             <1..>384
                     /locus_tag="ACHKG5_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0871193.1"
                     /translation="RLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYG
                     QQGEPAITTFRINAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTA
                     QVEKSARYLGSGSQWTVQGSRIKPG"
BASE COUNT           90 a          104 c          119 g           71 t
ORIGIN      
        1 tgcgcctgac cagtggaagc ggtcaggaca gccgtctggt gaccaccgcc atcactgcgg
       61 atacagagca tcgtttcagt ggtctgccgc tcggggaata caccctgaca gtcagggcaa
      121 ttaacagtta tggccagcag ggggaaccgg ccatcaccac cttccggatt aacgcgccag
      181 caaaacccgc caccattgaa ctgacgccgg ggtattttca gataacggcg gtaccggtgc
      241 tggcggtgta tgacccgacg gtgcagtttg agttctggtt ttcggaaaaa cgcatcacga
      301 acacggcaca ggtggaaaaa tctgcccgtt atctggggag cggcagtcag tggactgtcc
      361 agggaagccg gattaagccg ggga
//
LOCUS       DAZGXS010000516          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGXS010000516 DAZGXS010000000
VERSION     DAZGXS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            10..315
                     /gene="ynfD"
                     /locus_tag="ACHKG5_005331"
     CDS             10..315
                     /gene="ynfD"
                     /locus_tag="ACHKG5_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0871194.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT           97 a          123 c           81 g           82 t
ORIGIN      
        1 gagcttatga tgaaactctc aacctgttat gccgcgcttc tgctcgccct cgcctcaccc
       61 gcggtactcg ccgcgccggg atcctgtgag cgcatacaaa gcgatatatc acagcgcatt
      121 atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa tgaccaagtt
      181 gatcagcctg attcccaggt cgtcgggcat tgcgctaatg atacgcataa aattctctac
      241 acccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga tggcgcgcct
      301 gccgaaccgc agtaatacat tatcgactac acgccggata cgacacatcc ggcgatttgg
      361 acatacaaca taatcccacc ata
//
LOCUS       DAZGXS010000517          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGXS010000517 DAZGXS010000000
VERSION     DAZGXS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(1..305)
                     /locus_tag="ACHKG5_005332"
                     /pseudo
     CDS             complement(1..305)
                     /locus_tag="ACHKG5_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
BASE COUNT           96 a           98 c           90 g           87 t
ORIGIN      
        1 gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg ttctgggcct
       61 gattcagata aaaattaccc gacatggaaa tctcagacga cgccccccag accattgatg
      121 ttctcctgct ctgtggagca gagcgtggtc acatcaatat cctgtttctg cccggcggtg
      181 aactgcactt ccttgatggt gcagtccagg cgcagatatt ccgccttatc catagtttca
      241 gcagtcgccg gggcagatga aatcatcacc tgcgtcagct gtgagcgttc atacaaagca
      301 gacattctgc ctcctgataa taaaaaaccc gcacgcggcg gggtatgggt tttgtagaaa
      361 aaagaaaaag t
//
LOCUS       DAZGXS010000518          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGXS010000518 DAZGXS010000000
VERSION     DAZGXS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..111
                     /locus_tag="ACHKG5_005333"
     CDS             <1..111
                     /locus_tag="ACHKG5_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0871195.1"
                     /translation="RLHSELGMGFEERRVFRRYKGTGEQNTSGNARSKKC"
     gene            complement(193..>370)
                     /locus_tag="ACHKG5_005334"
     CDS             complement(193..>370)
                     /locus_tag="ACHKG5_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0871196.1"
                     /translation="FPNQMIEQINIALDQKGSGNFSAWVIEACRRRLINEKHAQFVPN
                     KDKHDQSTCSDRFT"
BASE COUNT          110 a           61 c           86 g          113 t
ORIGIN      
        1 agactacatt ctgagttggg aatgggattt gaagagcggc gggtattccg acgatataaa
       61 gggacaggag aacagaatac gtccggaaac gcgcggtcaa aaaaatgcta aaaaatatct
      121 gagagcgtta ttgcctgtta ccataagaaa aagcgacttt agtggtcgct ttttgtgtca
      181 tatataagtc gtttaagtaa acctgtctga acaggtgctc tggtcgtgtt tgtctttgtt
      241 gggtacaaat tgagcatgtt tttcattaat taatcttctt ctgcaggctt caataaccca
      301 cgctgaaaaa ttacctgaac ctttctgatc aagagcgatg ttaatttgtt caatcatttg
      361 gttaggaaag
//
LOCUS       DAZGXS010000519          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGXS010000519 DAZGXS010000000
VERSION     DAZGXS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>361)
                     /locus_tag="ACHKG5_005335"
     CDS             complement(<1..>361)
                     /locus_tag="ACHKG5_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFP0871197.1"
                     /translation="RLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAA
                     TQTFDGIAQNMAAMLTGA"
BASE COUNT           59 a          133 c           92 g           77 t
ORIGIN      
        1 ctgcaccggt cagcatcgcc gccatattct gtgcaatacc gtcaaaggtc tgcgtggcag
       61 cacttttaac ctgcgaaaaa ctgtccgtcg cactttccgc ccactcgccc cagccggact
      121 tcagcccggc catccagctt ccacgaagct gctcctccgc agaccaggtg ttcttcagtg
      181 ccgatgtggc cttcgccagc gcagccggat tatcaccgta cacgtcccga agacgctgcg
      241 cttcagactc ccgctgcgcc tgacggtcag tgagaccgcg ggcttttgcg ctgatggcgg
      301 cctgcttcgc gctctgctgc tcttcaaacc gcaccgcctg ctgtgccagc tcattcaggc
      361 g
//
LOCUS       DAZGXS010000520          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGXS010000520 DAZGXS010000000
VERSION     DAZGXS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..300)
                     /locus_tag="ACHKG5_005336"
     CDS             complement(<1..300)
                     /locus_tag="ACHKG5_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0871198.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFK"
BASE COUNT           87 a           95 c           89 g           88 t
ORIGIN      
        1 cttaaacgca tacaccgtgt cattgtcata ggcatcacgc agggcgttct gggcctgatt
       61 cagataaaaa ttacccgaca tggaaatctc ggacgacgcc cccagaccgt tgatgttctc
      121 ctgctctgtg gagcagagcg tggtcacatc aatatcctgt ttctgaccgg cggtgaactg
      181 gacttccttg atggtgcagt ccaggcgcag atattccgcc ttctccatgg tttcagcagt
      241 cgccggggca gatgaaatca tcacctgcgt cagctgtgaa cgttcataca aagcagacat
      301 tctgcctcct gataataaaa aacccgcacg cggcgggtta tgggttctgt tgaaaaaat
//
LOCUS       DAZGXS010000521          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGXS010000521 DAZGXS010000000
VERSION     DAZGXS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>356
                     /locus_tag="ACHKG5_005337"
     rRNA            <1..>356
                     /locus_tag="ACHKG5_005337"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          108 a           80 c          113 g           55 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag gagcccagag cctgaatcag
      121 tgtgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgt
//
LOCUS       DAZGXS010000522          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGXS010000522 DAZGXS010000000
VERSION     DAZGXS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>355
                     /locus_tag="ACHKG5_005338"
     CDS             <1..>355
                     /locus_tag="ACHKG5_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP0871199.1"
                     /translation="GKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEV
                     FDKIKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLT
                     LDNYLDDLHFDNSPLV"
BASE COUNT          122 a           54 c           70 g          109 t
ORIGIN      
        1 tggcaaaggg gatttattta aaaaacgtac actgtgggag tttgttcgcg atctttttcc
       61 tggctctcat attaaagaag tcaaaggctt aatttacgaa tttgtcacga aagttgataa
      121 taaagccgag gtgttcgata agattaaatc tctggcaaaa aaagaacaac aatggcgatt
      181 tagtactaag acagacttta ccacaaatga aaataatgaa gttattgtga gtagatcctt
      241 taatctctac acgggggcta cttcgaatga taacgaaaaa aaacaggttt cttcagagag
      301 attaacgctt gataattatc tcgatgattt acattttgat aattctccct tggtg
//
LOCUS       DAZGXS010000523          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGXS010000523 DAZGXS010000000
VERSION     DAZGXS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>353
                     /locus_tag="ACHKG5_005339"
     CDS             <1..>353
                     /locus_tag="ACHKG5_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0871200.1"
                     /translation="DRTSECLSEEIKIYQECHEKFIKFLKANFNQEIYPELYTPEIFY
                     EACRNLQSFYDHQETSQNAKYSAIVKKKSYFNKEIRNLIKKNIYPELYNEQCNKIPAS
                     STDDNQKITWQNFKT"
BASE COUNT          150 a           59 c           54 g           90 t
ORIGIN      
        1 tgacagaaca agcgaatgtc tttctgaaga gattaagata tatcaagaat gccatgaaaa
       61 atttattaaa tttttaaagg caaacttcaa tcaggaaata tacccagagc tatacactcc
      121 tgaaatattt tatgaggcct gcagaaatct ccagtcattt tatgatcatc aggagacgtc
      181 acaaaacgct aagtacagtg caatagtcaa gaaaaaaagt tattttaata aggaaatcag
      241 aaatctaata aaaaagaata tttatcctga gctatacaat gagcaatgca ataaaatccc
      301 cgcctcatcg acagatgata accagaaaat aacatggcag aattttaaaa cta
//
LOCUS       DAZGXS010000524          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGXS010000524 DAZGXS010000000
VERSION     DAZGXS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>352
                     /locus_tag="ACHKG5_005340"
     CDS             <1..>352
                     /locus_tag="ACHKG5_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0871201.1"
                     /translation="CQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWL
                     FGKQCKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHD
                     PHFLPVIFEHPPEMV"
BASE COUNT           77 a           97 c          107 g           71 t
ORIGIN      
        1 ctgccaggta cagacccata tccggaccat cacccaccgg gtgacggaca ccaccctgaa
       61 ggtggtggca gccgatccga atacggtgtc cggtatcaag tccgtgggta cgctgattga
      121 tgagttgtgg ctgtttggca agcagtgcaa ggcggaggac atgttacgtg aagccatagg
      181 cggccttgcc tcccgcccgg aagggtttgt ggtgtatacg accacccagt cgaatgaacc
      241 gcccgccggg gtgttcagac agaaactgca gtacgcccgg gatgtgcgcg acggcaaaat
      301 tcatgatccg cactttctgc cggtgatatt tgaacaccct cctgaaatgg tg
//
LOCUS       DAZGXS010000525          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGXS010000525 DAZGXS010000000
VERSION     DAZGXS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..116
                     /locus_tag="ACHKG5_005341"
     CDS             <1..116
                     /locus_tag="ACHKG5_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HFP0871202.1"
                     /translation="FEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(141..>346)
                     /locus_tag="ACHKG5_005342"
     CDS             complement(141..>346)
                     /locus_tag="ACHKG5_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871203.1"
                     /translation="KRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNV
                     AWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT           99 a           72 c           74 g          101 t
ORIGIN      
        1 actttgagtt tatgccagaa ctgaagtgga gtttcggcta cccaggcgcg attatcttta
       61 tgatcctcgc gggcctggca ccgtatctgt actttaagcg caagaactgg ctgtaactat
      121 acgatgtcgc tgtgagtaag ttagtgtgct gacagcgaca ttttagcaga gggaatcgca
      181 gataacataa ttgttgtgaa taatatccat gccacgttga gttggacgct taatgactca
      241 catctcaccc cttcaattgc tgcagagata atagaaaaaa gtgtcaaacc caatgaaatc
      301 aatacaaagt agattgcctt acatccagta tctcttattc tttttg
//
LOCUS       DAZGXS010000526          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGXS010000526 DAZGXS010000000
VERSION     DAZGXS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>342)
                     /locus_tag="ACHKG5_005343"
     CDS             complement(<1..>342)
                     /locus_tag="ACHKG5_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871204.1"
                     /translation="QTAKLEFFCDEFLKISQSRYGLSTSTDSIANLLAFFTKASDAID
                     SIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFTRGENNEGILSVESKE
                     GFISQRTINLNE"
BASE COUNT           97 a           59 c           62 g          124 t
ORIGIN      
        1 ttcatttaag tttattgttc tttgtgaaat aaacccttct ttgctttcga cactcaaaat
       61 accttcgttg ttttcacctc gggtaaaggt aagggtatag ggttcataat ccgctgcaaa
      121 ttcattttta tacgttatga catgttcgac aaaatgtctt attggtacga aaccataact
      181 gtgaacgtca attttttggg tcttgatact atcaattgca tctgaggctt tcgtgaaaaa
      241 agccaataaa ttagctatgc tgtctgttga ggttgataac ccgtatctac tttgagatat
      301 ttttagaaac tcatcgcaaa aaaactcaag tttggcggtt tg
//
LOCUS       DAZGXS010000527          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGXS010000527 DAZGXS010000000
VERSION     DAZGXS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>341
                     /locus_tag="ACHKG5_005344"
     rRNA            <1..>341
                     /locus_tag="ACHKG5_005344"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           86 a           87 c           98 g           70 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac c
//
LOCUS       DAZGXS010000528          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGXS010000528 DAZGXS010000000
VERSION     DAZGXS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>339
                     /locus_tag="ACHKG5_005345"
     CDS             <1..>339
                     /locus_tag="ACHKG5_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0871205.1"
                     /translation="VFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPA"
BASE COUNT           79 a           89 c          126 g           45 t
ORIGIN      
        1 ggtgttccac acgcggatac agcacggcga accacaggtg gagagccggt acctctatga
       61 cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg gagcgtgacc tgacggggtg
      121 gatgtcgctg tcgcgtaaac cggaggagac ctggtacggg tgggacggtg acaggctgac
      181 cactgtacag acccaacaga caagaatcca gacggtatac cagccgggaa gcttcacgcc
      241 gctcctgaga atcgaaacag agaatggtga acaggcgaag gcgcggcacc gtagcctggc
      301 ggaggtgttg caggaggaca cgggtgtgac gctaccggc
//
LOCUS       DAZGXS010000529          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGXS010000529 DAZGXS010000000
VERSION     DAZGXS010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>333
                     /locus_tag="ACHKG5_005346"
     CDS             <1..>333
                     /locus_tag="ACHKG5_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0871206.1"
                     /translation="TQERLNRNIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLV
                     MLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQN
                     RLIDEQASR"
BASE COUNT           84 a           83 c          111 g           55 t
ORIGIN      
        1 acacaggaac gcctgaacag aaatattgct gccagaagcg ccgcccagaa tgcgctgaac
       61 agtacaacgg cggtgggctc acgtctgatg agcggtgcgc tggggctggt tggtggcgta
      121 cccggactgg tgatgctggg ggctgcagca tggtacacgc tgtaccagaa tcaggagcag
      181 gccagggagt ctgcgcgcca gtatgcactg acgatagatg aaatcgcgca taaaacgccg
      241 tcaatgtctt tgcctgaagc ctcagataat gaaggacgaa cacgggcggc gctgacagag
      301 cagaaccggc tgattgatga acaggccagt cgg
//
LOCUS       DAZGXS010000530          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGXS010000530 DAZGXS010000000
VERSION     DAZGXS010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>333
                     /locus_tag="ACHKG5_005347"
     CDS             <1..>333
                     /locus_tag="ACHKG5_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0871207.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZGXS010000531          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGXS010000531 DAZGXS010000000
VERSION     DAZGXS010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.535"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..165
                     /locus_tag="ACHKG5_005348"
     CDS             <1..165
                     /locus_tag="ACHKG5_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871208.1"
                     /translation="EEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRP
                     SRTFQQRIWR"
     gene            167..>326
                     /locus_tag="ACHKG5_005349"
     CDS             167..>326
                     /locus_tag="ACHKG5_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cons domain protein"
                     /protein_id="HFP0871209.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAV"
BASE COUNT           74 a           93 c           88 g           71 t
ORIGIN      
        1 gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga ggttaaaaaa
       61 gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta ctccgaacgg
      121 ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaatgtgc gggtattact
      181 tcgacctgtt ctggtaccgg aactcggtct ggttatcgtt aagccaggcc gtgaatcaat
      241 gtcagcattc cataacggca gaatactggt ggagccggaa ccaaaaagca tgcgagctct
      301 gccgtccggg gttgtacctg ccgttc
//
LOCUS       DAZGXS010000532          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGXS010000532 DAZGXS010000000
VERSION     DAZGXS010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.536"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>326
                     /locus_tag="ACHKG5_005350"
     CDS             <1..>326
                     /locus_tag="ACHKG5_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0871210.1"
                     /translation="SIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHPA
                     PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAI
                     VDNGAR"
BASE COUNT           64 a          106 c          101 g           55 t
ORIGIN      
        1 tccatcgatg ccgccagccg caccctgacg ctggaccgtg aggtgacact gccggagacc
       61 ggtgccgcca cggtgaacct gattaacggc agcggtaagc cggtgagtgt ggacatcacc
      121 gcacaccccg cgccggaccg gatacaggtc agcaccctgc ctgatggtgt ggagacatac
      181 ggtgtatggg gactatccct gccgtcactg cgccgtcgcc tgttccgctg tgtctccgtc
      241 cgggaaaaca cggacggcac ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa
      301 gccatcgtgg ataacggtgc ccgctt
//
LOCUS       DAZGXS010000533          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGXS010000533 DAZGXS010000000
VERSION     DAZGXS010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.537"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>307
                     /locus_tag="ACHKG5_005351"
     CDS             <1..>307
                     /locus_tag="ACHKG5_005351"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--(glucosyl)LPS
                     alpha-1,2-glucosyltransferase"
                     /protein_id="HFP0871211.1"
                     /translation="RIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVP"
BASE COUNT          112 a           48 c           52 g           95 t
ORIGIN      
        1 gtcgcatata taagcggtta tttcaaaaat ggacaagact tgatcctctt ccatattcac
       61 agagaatatt gaatattgca catgattttc ctataacgaa agaaagtgtt attgtagttc
      121 ataatagcat gaagctatac actcaaattc gtaagcgagc tcctcaggca agagtggtta
      181 tacatatgca taatgcattt gaaccaaaat tgcttgagca aaatgttaaa atgatagtgc
      241 ctagtttata cttgaaaaag tattaccaat cttatttagc aaatgcagat attgagattg
      301 taccaaa
//
LOCUS       DAZGXS010000534          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGXS010000534 DAZGXS010000000
VERSION     DAZGXS010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.538"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
BASE COUNT          128 a           65 c           37 g           73 t
ORIGIN      
        1 gccaggtgat catgtagcat aagtaacctc cgtttatcta cagactgcca tcctgaattt
       61 accgtcctta ataagacaac aataaaaaac taattatata aaaacaataa aaataatata
      121 caaaaaaact aaacaacaaa gaaaagcaaa tcatatctgg cattttaatt gaaagagcaa
      181 ttaccgaaca aaagacgctg actatatact caaaaccaaa caacaattct gccaatcagg
      241 tatcatggca acacacggaa ttaccatgtt tttgccttct ctgcccatac aatacgggca
      301 tat
//
LOCUS       DAZGXS010000535          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGXS010000535 DAZGXS010000000
VERSION     DAZGXS010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.539"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            68..>302
                     /gene="dinJ"
                     /locus_tag="ACHKG5_005352"
     CDS             68..>302
                     /gene="dinJ"
                     /locus_tag="ACHKG5_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP0871212.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDAD"
BASE COUNT           86 a           71 c           74 g           71 t
ORIGIN      
        1 attgtcgctg ttgctcattt gagctacaat tcaagctgaa taaatataca gcataggaga
       61 tatcccaatg gctgctaacg catttgttcg cgcccgaatc gatgaagatc tgaagaatca
      121 ggcggcggac gtactggccg ggatggggct gaccatctct gacctggttc gcataaccct
      181 cacaaaggtc gcgcgtgaaa aggcattgcc gtttgattta cgcgagccta atcaattaac
      241 cattcaatca atcaaaaaca gcgaagctgg cgttgatgtt cataaggcca aagacgccga
      301 tg
//
LOCUS       DAZGXS010000536          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGXS010000536 DAZGXS010000000
VERSION     DAZGXS010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.540"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>302)
                     /locus_tag="ACHKG5_005353"
     CDS             complement(<1..>302)
                     /locus_tag="ACHKG5_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP0871213.1"
                     /translation="LKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSL
                     NELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQR"
BASE COUNT          110 a           67 c           37 g           88 t
ORIGIN      
        1 gtctttgttt taaccattcc cgtaaattaa tatacattac accagagtta aaatagctac
       61 cgttaaaatc ctcattacac aaacgctcag cgcttttact ttgcatagca tctacatcaa
      121 ttaccactgc cccatattca tctttaaact ctaatgctat taactcgttc aatgaaccct
      181 tacagacgat atcagcatca agatataata atgaatccaa tcgcgcagaa aaataatcaa
      241 atgaaagcaa acgataataa atagatactg gccagatatt tgattgaggt agcgccttca
      301 at
//
LOCUS       DAZGXS010000537          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGXS010000537 DAZGXS010000000
VERSION     DAZGXS010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.541"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(<1..>301)
                     /locus_tag="ACHKG5_005354"
     CDS             complement(<1..>301)
                     /locus_tag="ACHKG5_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP0871214.1"
                     /translation="ICLLSGCHFYRERFAERGFFYKVPDVLRDYLSAIPLEINEKARY
                     KPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILSPLPLASTLLE"
BASE COUNT           98 a           53 c           61 g           89 t
ORIGIN      
        1 tctctaaaag tgttgatgca agtggtaacg gcgatagtat gtggtcaaca aaatcaggaa
       61 ataataaatt aaatcgttgc tgcattgcca atggttgttg ccttatatag ggtaacaacg
      121 ttgaaaaacc acatgtgatg atattgtgat agttagcgat gccgggtttg tatcgtgcct
      181 tttcatttat ttccagcggt atagctgata aataatccct tagtacatca ggaaccttat
      241 aaaaaaaccc gcgttctgca aacctttctc gataaaagtg acaaccactg agtaaacaga
      301 t
//
LOCUS       DAZGXS010000538          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGXS010000538 DAZGXS010000000
VERSION     DAZGXS010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.542"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            16..>300
                     /locus_tag="ACHKG5_005355"
     CDS             16..>300
                     /locus_tag="ACHKG5_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0871215.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEH"
BASE COUNT           88 a           80 c           73 g           59 t
ORIGIN      
        1 gccacctgat taaaggtgat atcctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gtactttcac ccctgaattc aggctggaat gtgcacagct aattgttgat
      121 aagggctact catatcgaca agccagtgaa gcgatgaatg tcggttctac cacgcttgag
      181 agttgggtgc gccagctcag gcgagagcgt caggggattg cgccctctgc cacacctatt
      241 actccagacc agcaacgtat ccgcgaactg gaaaagcagg ttcgccgcct ggaggaacac
//
LOCUS       DAZGXS010000539          286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGXS010000539 DAZGXS010000000
VERSION     DAZGXS010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.543"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            13..>286
                     /gene="espW"
                     /locus_tag="ACHKG5_005356"
     CDS             13..>286
                     /gene="espW"
                     /locus_tag="ACHKG5_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP0871216.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAIN"
BASE COUNT           97 a           65 c           50 g           74 t
ORIGIN      
        1 aggagactat ttatgcccaa aatatcatca gttgtatcat catgttacca tctgttcagt
       61 gaacatcaac aactttcaaa tgaaacaaca atgacgaacc ccgtctccag aagaattgtt
      121 cataaagaat atggtatatc tttaaaatcc gttcctgtat ggttggctac agctaaaact
      181 ccccttgctc tactcaatgg cagacataca agaagtcact catttattat tgcagggacc
      241 ccaggaatgg gaagcaggag cggagcccaa tactatgcca taaaca
//
LOCUS       DAZGXS010000540          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGXS010000540 DAZGXS010000000
VERSION     DAZGXS010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.544"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            <1..>282
                     /locus_tag="ACHKG5_005357"
     CDS             <1..>282
                     /locus_tag="ACHKG5_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFP0871217.1"
                     /translation="CLYAACLGELTGGNPLYDCAIDTHTTILSGNLAANTQVKIPVYM
                     YHRGAIAEKDILPFMEKVTNIYSRIEDIYKYSNNMKNNYKKALADLQSLL"
BASE COUNT           95 a           43 c           51 g           93 t
ORIGIN      
        1 tgtttatatg ctgcttgttt gggcgagtta accggtggga atcccttgta tgattgtgct
       61 attgatactc ataccactat cttaagtggt aatcttgctg caaacactca ggttaaaatt
      121 cctgtttata tgtaccatag aggagctata gctgaaaaag acattttacc tttcatggaa
      181 aaggtaacga atatttattc acggatagaa gatatttata aatatagcaa taatatgaag
      241 aataattaca aaaaggcact agccgattta caatccttat ta
//
LOCUS       DAZGXS010000541          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGXS010000541 DAZGXS010000000
VERSION     DAZGXS010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.545"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
BASE COUNT           90 a           47 c           48 g           97 t
ORIGIN      
        1 ttcttcatat caaacacagt gcatgtgttg cacattatcc ctggaacgtt ggaggcctaa
       61 tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg tggcttttgc aatacaattc
      121 ttacgcctgt aggattagtg agaagactta tagtgctaac ttgaaactat aaatcatcgg
      181 tataatccct gattttattg ttgacatttc atatatgccg actatttata tggtatactt
      241 gtcgaattat cttaaaggaa gctcaaattt tcttattttt at
//
LOCUS       DAZGXS010000542          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGXS010000542 DAZGXS010000000
VERSION     DAZGXS010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.546"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(106..>279)
                     /locus_tag="ACHKG5_005358"
     CDS             complement(106..>279)
                     /locus_tag="ACHKG5_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0871218.1"
                     /translation="DGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFETQSRNVTD
                     WMPLPEPPQEVRQ"
BASE COUNT           84 a           73 c           54 g           68 t
ORIGIN      
        1 ttggtgtgaa tcccggcgaa cgtttttacc cccaccgaca aataacatat actaaaaaat
       61 caatagctat agcaacgcct gtaattgcaa aggcttcagg ccagatcatt ggcgcacctc
      121 ctgcggcggt tctggtagcg gcatccagtc ggttacattg cggctctgtg tttcgaaaaa
      181 ttcatcacca ttacggacta catcaaaaaa ctcaccgtct cgatattgcg cataaagaac
      241 gaatgcgcca tcacataaaa taattacgtg ctgaccatc
//
LOCUS       DAZGXS010000543          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGXS010000543 DAZGXS010000000
VERSION     DAZGXS010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.denovo.547"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            24..100
                     /locus_tag="ACHKG5_005359"
     tRNA            24..100
                     /locus_tag="ACHKG5_005359"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHKG5_005360"
     tRNA            143..218
                     /locus_tag="ACHKG5_005360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZGXS010000544        29078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.guided.050, whole genome shotgun sequence.
ACCESSION   DAZGXS010000544 DAZGXS010000000
VERSION     DAZGXS010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.guided.050"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKG5_005361"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKG5_005361"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0871219.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKG5_005362"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKG5_005362"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0871220.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKG5_005363"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKG5_005363"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0871221.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKG5_005364"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKG5_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0871222.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTITWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKG5_005365"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKG5_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0871223.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKG5_005366"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKG5_005366"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0871224.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKG5_005367"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKG5_005367"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0871225.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKG5_005368"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKG5_005368"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0871226.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKG5_005369"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKG5_005369"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0871227.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKG5_005370"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKG5_005370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0871228.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHKG5_005371"
     CDS             12901..14034
                     /locus_tag="ACHKG5_005371"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0871229.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14122..14655
                     /gene="blc"
                     /locus_tag="ACHKG5_005372"
     CDS             14122..14655
                     /gene="blc"
                     /locus_tag="ACHKG5_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0871230.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHKG5_005373"
     CDS             complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="ACHKG5_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0871231.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="ACHKG5_005374"
     CDS             complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="ACHKG5_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0871232.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="ACHKG5_005375"
     CDS             complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="ACHKG5_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0871233.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15579..16145)
                     /gene="efp"
                     /locus_tag="ACHKG5_005376"
     CDS             complement(15579..16145)
                     /gene="efp"
                     /locus_tag="ACHKG5_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0871234.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16187..17215
                     /gene="epmB"
                     /locus_tag="ACHKG5_005377"
     CDS             16187..17215
                     /gene="epmB"
                     /locus_tag="ACHKG5_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0871235.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17450..18319
                     /locus_tag="ACHKG5_005378"
     CDS             17450..18319
                     /locus_tag="ACHKG5_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0871236.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18512..18865)
                     /locus_tag="ACHKG5_005379"
     CDS             complement(18512..18865)
                     /locus_tag="ACHKG5_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0871237.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19003..20658)
                     /gene="groL"
                     /locus_tag="ACHKG5_005380"
     CDS             complement(19003..20658)
                     /gene="groL"
                     /locus_tag="ACHKG5_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0871238.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            complement(20702..20995)
                     /locus_tag="ACHKG5_005381"
     CDS             complement(20702..20995)
                     /locus_tag="ACHKG5_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0871239.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21271..22527
                     /gene="yjeH"
                     /locus_tag="ACHKG5_005382"
     CDS             21271..22527
                     /gene="yjeH"
                     /locus_tag="ACHKG5_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0871240.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22543..23019)
                     /locus_tag="ACHKG5_005383"
     CDS             complement(22543..23019)
                     /locus_tag="ACHKG5_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0871241.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23356..24792
                     /gene="aspA"
                     /locus_tag="ACHKG5_005384"
     CDS             23356..24792
                     /gene="aspA"
                     /locus_tag="ACHKG5_005384"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0871242.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            24910..26211
                     /gene="dcuA"
                     /locus_tag="ACHKG5_005385"
     CDS             24910..26211
                     /gene="dcuA"
                     /locus_tag="ACHKG5_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0871243.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26327..26665
                     /gene="cutA"
                     /locus_tag="ACHKG5_005386"
     CDS             26327..26665
                     /gene="cutA"
                     /locus_tag="ACHKG5_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0871244.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26641..28338
                     /gene="dsbD"
                     /locus_tag="ACHKG5_005387"
     CDS             26641..28338
                     /gene="dsbD"
                     /locus_tag="ACHKG5_005387"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0871245.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28375..28950
                     /locus_tag="ACHKG5_005388"
     CDS             28375..28950
                     /locus_tag="ACHKG5_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0871246.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         7109 a         7598 c         7376 g         6995 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaattt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 agcaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgtttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctgaccg atgctgttac
      481 cgcaaatcgg cgatttaccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagcca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gcctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatttagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcgc
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcgtagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggga cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 ctgaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gatacgctac tgctggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaagatgcg ctgaaagaag tgaacgaagc gacccaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatca cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtcacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc tcgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccaacgacgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcag tccttgcggc tagtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtga caattcgcga tcttactggt agcgtgacga
     5881 aaattaatac ccgcgcaacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gctgaataat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag aacgacaacc accgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatagcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tgatcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggttgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggtgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcacca agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcaacaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggg
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgaa
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacgagcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    14161 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgaccgt agtgaataat
    14221 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgcttt
    14281 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
    14341 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
    14401 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    14461 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggaccg
    14521 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
    14581 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    14641 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    14701 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
    14761 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
    14821 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
    14881 gtcaaacgac taaagccgtg ggtatatttc aggcctacgg cccataccac ttccagcaga
    14941 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
    15001 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
    15061 ggtggcgggg gaatttcaac tattaccttt gtgactatcg cagaaaaaac aaacggcacg
    15121 acacagctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc
    15181 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
    15241 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
    15301 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
    15361 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgggag
    15421 atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg
    15481 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
    15541 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
    15601 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
    15661 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
    15721 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
    15781 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcgttg
    15841 tcgccaattg cttttgcgtc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
    15901 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
    15961 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
    16021 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
    16081 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
    16141 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
    16201 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
    16261 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
    16321 caacgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
    16381 aggcaatccg gacgatccgc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
    16441 cgcgcccgga ttctccaccg acccgctgga agaacagcac agcgtagtgc ctggtttgtt
    16501 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
    16561 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
    16621 aactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
    16681 cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat
    16741 cccgcatatc aaacgtctgc ggattcacag tcgtctgccg attgtgatcc cggcacgcat
    16801 cacagatgcg ctggttgaac gcttttcaca ttctacgctg caaatcttgc tggtgaatca
    16861 catcaaccat gccaatgaga tagatgaaac attccgtcag gcgatggcta agttgcgccg
    16921 ggcgggcgtt actttgctga accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
    16981 aacgctggca aacctgagta atgcgttgtt cgatgcaggc gtgatgccgt attacctgca
    17041 tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
    17101 gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga
    17161 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
    17221 taaacaggga agtttaatct ttgcccgtta cataaccttt aattattcat atacattgct
    17281 gttgttactc tttatataac caacaccaat aattaacctt tcttttctgt ttatatagag
    17341 tgagtacggc tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt
    17401 ataaattaaa attaattatt taattgagaa atggttaggg agaacctaca tggccataag
    17461 catcaagggt gttaataccg gtgttattcg caagagtaat aatttcattg cactggcgct
    17521 gaaaattaaa gaacctcgca ataaagaatc gttgttcttt atgtctgtca tggaactgcg
    17581 cgacctgctg attgcgctgg aaagtcgtct gcatcaaaag cataaactgg atgctgctgc
    17641 acgccttcag tatgagcaag ctcgcgataa agtcattaag aaaatggcag aaaacatccc
    17701 agaaattctg gttgatgagc ttaaaaatgc cgatattaat cgtcgggtta acacgctgga
    17761 acttaccgat aatcaaggcg aaaatcttac cttcgtatta acgctacatg acggtagcaa
    17821 atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc
    17881 aattaataat gcggaaatgc gtgaactggc attacgtatt acctcactgt tagatttcct
    17941 gccgttatat gacgttgact gccagcaaaa tggcaatctg gaatatgaca cctattccca
    18001 accggaatgg aaacataact tgttcgacca ttatctggcg gtgctttacc gctttaaaga
    18061 tgaaagtggc aaagaacaat ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg
    18121 taaagaaata gaggcgatca ctcgccgaat gcttgatttc agcccacgac tgaaaaaact
    18181 cgtgggtgtg ccatgtcagg tctatgttcg caccgtagcg gcgaataacg cccaaccgct
    18241 aacccaggat caatgcttac gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac
    18301 tgcaccacag gcgaaataat ctcatctttg ctaatgcgag gctggagcca tccagcctga
    18361 aatgactgat aacgtgatcg ttggtcatgc cggatgcggc gtaaacgcct tatccggcct
    18421 acaaattctc aagcattcaa tcagttgcaa tgccttatgt aggcctgata agcgaagcgc
    18481 atctggcaat tatacgtttg tcaccatcac atcagttcgg gcacttatat acctggccga
    18541 taatctggct atccgtcggg acaaaactgg acaacattcc ctgcgacggg ctactgatgc
    18601 cataaatcac gttaccgccc attgcagccg cctggttgcg cagatcgttt gctgcgccgc
    18661 gcatagaacc gccctcttct ccgtgttgcc cggaaagcca gttgctttgc ttacctgtcg
    18721 cagtaccaat cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt acactctgac
    18781 cggcagcact caattcgttg ctggagctac aacccgccat cagtagcgcg gcaccgacaa
    18841 tccctgctaa gtattttacg tgcatgttat tccccataat caatgagctg gacgccactc
    18901 gcgtcgtccg tgtctgaatc ttatactaga aagatgacca aaagaaaaac ccccagacat
    18961 ttctgcccgg gggtttgttt atttctgcga ggtgcagggc aattacatca tgccgcccat
    19021 gccacccatg ccacccatgc cgcccatacc gccagcagcg cctaagtcag ctgcatcgtt
    19081 tttcggcagg tcggtaacca tgcattcggt ggtgatcatc aggccagcca cagaagccgc
    19141 gtactgcaga gcagaacggg ttactttggt tgggtccagg atacccatgt cgatcatgtt
    19201 gccgtattct tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt taacggtgtt
    19261 agcaacaaca gacggttctt cgccgcagtt caggacgatc tgacgcagcg gagcttccat
    19321 tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc
    19381 cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc cttcttctac
    19441 cgcagcacgg gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt tcatttcaac
    19501 ttcggtagca gcacctactt tgataactgc aacgccgcct gccagtttcg ctacgcgctc
    19561 ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac ggatctgagc
    19621 aacacggccc tggattgcag cttcttcgcc cacgccatcg atgatggtgg tggtgtcttt
    19681 gttgatcaca acgcgtttag cctgacccag gtcttccagg gttgcttttt ccagctccat
    19741 accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct gcagcatagc
    19801 tttacgacga tcgccgaagc ccggagcttt aactgcagca actttcacga tgccacgcat
    19861 ggtgttaaca accagagttg ccagcgcttc gccttctaca tcttcagcga tgatcagcag
    19921 cggtttgcct gctttggcaa cggcttccag aaccggcagc atttcgcgga tgttggagat
    19981 tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag tttccggctt
    20041 gttgatgaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa ccacgtccag
    20101 ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac cgactttgtc
    20161 catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga tagtaccaac
    20221 ctgagcaatc gctttagagt cagagcacgg tacggacagc gctttcagtt cttcaactgc
    20281 agcggtaaca gctttgtcga taccacgttt caggtccatc gggttcatgc ccgcagcaac
    20341 agctttcaga ccttcagtga tgatagcctg agccagtacg gttgcagtgg tggtaccgtc
    20401 gcctgcagcg tcgttcgctt tagaggcaac ttctttcacc atctgcgcac ccatgttttc
    20461 gaacttgtct tccagttcga tttcacgagc aacggaaaca ccatctttgg tgatggtcgg
    20521 tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg taactttcac
    20581 tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt taccgaattt
    20641 tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg tcgtgcgagg
    20701 attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact tcttcattgt
    20761 cgatcttctc ggatttcaca ccgtagccat cgttgaaaat aacgatgtcg ccaactttca
    20821 catccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca gccagcactt
    20881 cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca gcagatttag
    20941 tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata ttcattgata
    21001 actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac gtggtttccc
    21061 ggctggtgac cagagaaatg gggatgaggc ttcgcccctt caagggggaa aaagaaaaaa
    21121 aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt catcgcaatt
    21181 tcttcatcgc cgtcataagc gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc
    21241 caaaatttgg gcgcaatcgg acatcaaccc atgagtggac tcaaacaaga actggggctg
    21301 gcccagggca ttggcctgct atcgacgtca ttattaggca ctggcgtgtt tgccgttcct
    21361 gcgttagctg cactggtagc gggcaataac agcctgtggg cgtggcccat tttgattatc
    21421 ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc cagcgcaggc
    21481 ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt caccggctgg
    21541 ctgtttttat cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc tgccggattc
    21601 ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact cggtacgctg
    21661 gcgttggtgt ggtatatcgg tactcgcggt gccagttcca gtgcaaatct acaaacagtt
    21721 attgccggac ttatcgtcgc actgattgtc gctatctggt gggcgggcga tatcaaacct
    21781 gcgagtatcc ccttccccgc gccaggaaat atcgaactta ccgggttatt tgctgcgtta
    21841 tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg cccatcttgc ctcggaattt
    21901 aaaaatccag aacgtgattt tcctcgtgct ttgatgattg gtctgctgct ggcaggatta
    21961 gtctactggg gctgtacggt agtcgtctta cacttcgacg cctatggtga acaaatggcc
    22021 gcggcagcat cgcttccaaa aattgtagta caactgttcg gtgtaggagc gttatggatt
    22081 gcctgcgtaa ttggctatct ggcctgcttt gccagtctca acatttatat acagagcttc
    22141 gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctggc acgcctctct
    22201 tctcgccata tcccgaataa tgccctcaat gcggtgctcg gctgctgcgt ggtgagcacg
    22261 ttggtgattc atgctttaga gatcaatctg gacgctctta ttatttatgc caatggcatc
    22321 tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca aggtcgttat
    22381 cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactggcaat ggtcggctgg
    22441 aaaagtctct atgcgctgat catgctggcg gggttatggc tgtttctgcc aaaacgaaaa
    22501 acgccggaaa atggcataac cacataatcc ggcgtttcga cattaatcct ggcgatcgtc
    22561 tttatgatca aggcggtcgc ggtcatcatc ctttcgctgg tactcaccat caaaagtatt
    22621 accgccaccg gtcccggcgc taaaaccgcc gcctggcatg cgagaaaagc gcaaatgcgg
    22681 catcaacttc actgtcagat gcttttgcac cggcggcaat aaaagcagaa gaccgaggaa
    22741 gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg aaacactttt
    22801 aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca gcacgaaatt
    22861 cttaaagccc tggttacgta ccagtgacat accgataact gacgtaaata taaccagcac
    22921 gagagtcagc aataccccca atacatgggc aacctgaata aagattgaaa tctcaatata
    22981 gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag
    23041 ccggttaaac ccggctacct gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat
    23101 tagcaaaatt tcaagatatt tgcgcaacta tttttggtag taatcccaaa gcggtgatct
    23161 atttcacaaa ttaataatta aggggtaaaa accgacactt aaagtgatcc agattacggt
    23221 agaaatcctc aagcagcata tgatctcgga tattcggtcg atgcagggga taatcgtcgg
    23281 tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa agcaaatcat tggcagcttg
    23341 aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg
    23401 gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat
    23461 atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa
    23521 aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc
    23581 atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta
    23641 gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc
    23701 aatatcggtc tggaactgat gggtcaccag aaaggtgaat atcagtacct gaacccgaac
    23761 gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca
    23821 gtttactctt ccctgattaa gctggtagat gcgattaacc aattgcgtga aggctttgaa
    23881 cgtaaagcgg tcgaattcca ggacatcctg aaaatgggtc gtacccagct gcaggacgca
    23941 gtaccgatga ccctcggtca ggaattccgc gctttcagca tcctgctgaa agaagaagtg
    24001 aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta accttggcgc aacagcaatc
    24061 ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa
    24121 gttactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc
    24181 gcttatgtta tggttcacgg agcgctgaaa cgcctggctg tgaagatgtc caaaatctgt
    24241 aacgacctgc gcttgctctc ttctggtcca cgtgccggcc tgaacgagat caacctgccg
    24301 gaactgcagg cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg
    24361 gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa
    24421 gcaggtcagc tgcagttgaa cgttatggag ccggtcattg gccaggctat gttcgaatcc
    24481 gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcact
    24541 gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg
    24601 aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt
    24661 aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat
    24721 attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa
    24781 agcgaacagt aatcgtacag ggtagtacaa ataaaaaagg cacgtcagat gacgtgcctt
    24841 ttttcttgtg agcagtaact taaaaataac aatctaatat caacttgtta aaaaacaagg
    24901 aaggctaata tgctagttgt agaactcatc atagttttgc tggcgatctt cttgggcgcc
    24961 agattggggg gaataggtat tggttttgca ggcggattgg gggtgctggt tcttgccgct
    25021 attggcgtta aacccggtaa catcccgttc gatgtcattt ccattatcat ggcggttatc
    25081 gccgctattt ctgccatgca ggttgctggc ggtctggact atctggttca tcagacagaa
    25141 aagctgctgc gccgtaaccc gaaatacatc acgatcctcg caccgatcgt gacctatttc
    25201 ctgactatct ttgctggtac tggcaacatc tctctggcga cactgccagt tatcgctgaa
    25261 gttgcgaagg aacaaggcgt taaaccttgc cgtccgctgt ctactgcagt ggtatccgcg
    25321 cagattgcga tcaccgcatc gccaatctca gcggcagtgg tttacatgtc ttccgtgatg
    25381 gaaggtcatg gcatcagcta cctccatctg ctctccgtgg tcatcccgtc caccctgctg
    25441 gcggttctgg tgatgtcctt cctggtcact atgctgttca actccaaact ctctgacgat
    25501 ccgatttatc gcaagcgtct ggaagagggc ctggttgaac tgcgcggtga aaagcagatt
    25561 gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt agttggcgtg
    25621 gttatctatg caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc gctgatgaac
    25681 accaccaacg caatcctgat catcatgctc agtgttgcaa ctctgaccac cgttatctgt
    25741 aaagtcgata ccgacaacat cctcaactcc agcaccttca aagcaggtat gagcgcctgt
    25801 atttgtatcc tgggtgttgc gtggctgggc gatactttcg tttccaacaa catcgactgg
    25861 atcaaagata ccgctggtga agtgattcag ggtcatccgt ggctgctggc cgtcatcttc
    25921 ttctttgctt ctgctctgct gtactctcag gctgcaaccg caaaagcact gatgccgatg
    25981 gctctggcac tgaacgtttc tccgctgacc gctgttgctt ctttcgctgc ggtgtctggt
    26041 ctgttcattc tgccgaccta cccgacgctg gttgctgcgg tacagatgga tgacacgggt
    26101 actacccgta tcggtaaatt cgtcttcaac catccgttct tcatcccggg tactctgggt
    26161 gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct tcatgctgta atgaccaatc
    26221 gcggggcgtt cacgccccgc tttctttctc gccgactaac atcctttccc cgtccgttgt
    26281 atagtgacct ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc ttgatgaaaa
    26341 aagttcgaat accgcgtctg tcgtggtgct atgtaccgca ccggatgaag cgacagccca
    26401 ggatttagcc gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct tgatccccgg
    26461 cgctacctct ctctattact gggaaggtaa gctggagcaa gaatacgaag tgcagatgat
    26521 tttaaaaact accgtatctc accagcaggc actgctggaa tgcctgaagt ctcatcatcc
    26581 atatcaaacc ccggaacttc tggttttacc tgttacacac ggagacacag attacctctc
    26641 atggctcaac gcatctttac gctgatcctg ctactttgca gcacttccgt ttttgccgga
    26701 ttattcgacg cgccgggacg ttcacaattt gtccccgtgg atcaagcctt tgcttttgat
    26761 tttcagcaaa accaacatga ccttaatctg acctggcaga tcaaagacgg ttactacctc
    26821 taccgtaaac agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt gcagctgccg
    26881 caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg agatttaccg cgatcggctg
    26941 acgcttcccg taaccatcaa ccaggcgagt gcgggagcga cgttaactgt cacctaccag
    27001 ggctgtgctg atgccggttt ctgttatccg ccagaaacca aaaccgttcc gttaagcgaa
    27061 gtggtcgcca acaacgcagc gtcacagcct gtgtctgttc cgcagcaaga gcagcccacc
    27121 gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg gtattggtat cgcctttacg
    27181 ccatgcgtgc tgccaatgta cccactgatt tctggcatcg tgctgggtgg taaacagcgg
    27241 ctctccactg ccagagcatt gttgctgacc tttatttatg tgcaggggat ggcactgact
    27301 tacacggcgc tgggtctggt agttgccgcc gcagggttac agttccaggc ggcgctacag
    27361 cacccatacg tgctcattgg cctcgccatc gtctttacct tgctggcgat gtcaatgttt
    27421 ggcttgctta ctctacaact cccctcctcg ctgcaaacgc gcctcacgct gatgagcaat
    27481 cgccaacagg gcggctcacc cggcggagtg tttattatgg ggacgattgc cggactgatc
    27541 tgttcaccat gcaccaccgc accgcttagc gcgattctgc tgtatatcgc ccaaagcggg
    27601 aacatgtggc tgggcggcgg cacgctttat ctctatgcgt tgggcatggg cctgccgctg
    27661 atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg gatggaacaa
    27721 gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg tcttcctgct ggagcgagtg
    27781 attggtgatg tatggggatt acgcttgtgg tcggcgctgg gtgtcgcatt ctttggctgg
    27841 gcctttatca ccagcctaca ggccaaacgc ggctggatgc gcgtggtgca aataatcctg
    27901 cttgcagcgg cattggttag cgtgcgccca cttcaggatt gggcatttgg tgcgacgcat
    27961 accgcgcaaa ctcagacgca tctcaacttt acacaaatca aaacggtaga tgagttaaat
    28021 caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag atctttatgc cgactggtgc
    28081 gtcgcctgta aagagtttga gaaatacacc ttcagcgacc cgcaggtgca aaaagcgtta
    28141 gcagacacgg tattacttca ggccaacgtc actgccaacg acgcacaaga tgtggcgctg
    28201 ttaaagcatc ttaatgtcct tggcctaccg acaattctct tttttgacgg acaaggccag
    28261 gagcatccac aagcacgcgt cacgggcttt atggatgctg aaaccttcag cgcacatttg
    28321 cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa tagcgtgcaa
    28381 cgtgaagatg tactgggaga agccctgaaa ttattagaat tacaagggat tgccaacacc
    28441 acgctggaga tggttgctga acgtgtggat tatccactgg acgagctacg ccgcttctgg
    28501 ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca gatagatgtc
    28561 tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg aacaaaagct gctggcacgt
    28621 tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc cgggctgtct gtttatcgct
    28681 gcctgtacgt tttatcccga tcctggccac cctattcatc aactggccga tcagcaaaaa
    28741 agcgcggcct acgatttcac ccacgaactg ttaaccacgc tggaagttga cgatccggcg
    28801 atggtagcaa agcagatgga actggtgctg gaaggctgtt taagccgaat gctggtgaat
    28861 cgtagccagg cggatgtcga caccgcacat cggctggcgg aagatatcct gcgcttcgcc
    28921 cgctgccgcc agggtggtgc actgacctga cagaaacaca gaaaagaagc gatttgccgc
    28981 aatcttaagc agttgaatcg cttttactga aattaggttg acgagatgtg cagattacgg
    29041 tttaatgcgc cccgttgccc ggatagctca gtcggtag
//
LOCUS       DAZGXS010000545         6092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.guided.201, whole genome shotgun sequence.
ACCESSION   DAZGXS010000545 DAZGXS010000000
VERSION     DAZGXS010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.guided.201"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            99..302
                     /locus_tag="ACHKG5_005389"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHKG5_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHKG5_005390"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHKG5_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKG5_005391"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKG5_005391"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0871247.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKG5_005392"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKG5_005392"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0871248.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKG5_005393"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKG5_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0871249.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHKG5_005394"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHKG5_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0871250.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHKG5_005395"
     CDS             complement(4889..5944)
                     /gene="phoE"
                     /locus_tag="ACHKG5_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0871251.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1492 a         1497 c         1539 g         1564 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     5521 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcggtc caggcttcca
     5581 cgtcatacaa cgcgcccagg ttacggccat agtcgaaaga acccaaatct ttatacttca
     5641 atccggcaaa agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg
     5701 caaactccgc ttcccaacga ccataaccag tcagttgatc gttaatttgt gtttcgcctt
     5761 taaaaccaaa acggatataa ctctggtctc catctttact gtcgttatca ctcatataat
     5821 gcatggcttt aactttgcca tagacatcca gtttattacc gtctttgtta tatatttctg
     5881 cggcctgtac ggatgcagat gccacaatgc ccatcaccac taatgccaga gtgctctttt
     5941 tcattttcat tcctgatttt aattaacgcg cgaatattct gcgggagagc cccgttgaaa
     6001 acaggaaagt ttttaacctg agattgttaa agatatatta cagattaatg atattcttaa
     6061 aatgtggtaa tttattaaat ctgtaataaa ag
//
LOCUS       DAZGXS010000546         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203W46868
            SAMN43267862-rid23070363.guided.309, whole genome shotgun sequence.
ACCESSION   DAZGXS010000546 DAZGXS010000000
VERSION     DAZGXS010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267862
            Sequence Read Archive: SRR30307227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350545.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350545.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:30:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,398
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 102 of 238
            Pseudo Genes (incomplete)         :: 120 of 238
            Pseudo Genes (internal stop)      :: 69 of 238
            Pseudo Genes (multiple problems)  :: 43 of 238
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267862-rid23070363.guided.309"
                     /serotype="O157:H 7"
                     /isolate="2203W46868"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx2c"
     gene            95..1054
                     /locus_tag="ACHKG5_005396"
     CDS             95..1054
                     /locus_tag="ACHKG5_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP0871252.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1066..1335
                     /locus_tag="ACHKG5_005397"
     CDS             1066..1335
                     /locus_tag="ACHKG5_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP0871253.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1632..1955)
                     /locus_tag="ACHKG5_005398"
     CDS             complement(1632..1955)
                     /locus_tag="ACHKG5_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0871254.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          541 a          407 c          498 g          612 t
ORIGIN      
        1 cacacttatt ttccctggct cgcttttgcg ggcctttttt atatctgcgc cgggtctggt
       61 gctgattact tcagccaaaa ggaacacctt gtatatgaag tgtatattat ttaaatgggt
      121 actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta cgatagactt
      181 ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga tatcgacccc
      241 tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca ccccaccggg
      301 cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt ttgaccatct
      361 tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata cggcaacaaa
      421 tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga caacggtttc
      481 catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg aacgttccgg
      541 aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg agttcagtgg
      601 taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg tcacagcaga
      661 agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg aaactgctcc
      721 tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc gaatcagcaa
      781 tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat cctttaataa
      841 tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc agggggcgcg
      901 ttctgttcgc gccgtgaatg aagatagtca accagaatgt cagataactg gcgacaggcc
      961 cgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt ttctgaacag
     1021 aaagtcacag tttttatata caacgggtaa ataaaggagt taagtatgaa gaagatgttt
     1081 atggcggttt tatttgcatt agtttctgtt aatgcaatgg cggcggattg cgctaaaggt
     1141 aaaattgagt tttccaagta taatgagaat gatacattca cagtaaaagt ggccggaaaa
     1201 gagtactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca gttgacagga
     1261 atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc tgaagtgcag
     1321 tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt gtaatctgtg
     1381 tcacaattca gtcagttgac agttgcctgt cagactgagc atttgttaaa aaaattcgca
     1441 tggtgaatcc ccctgtgcgg aggggcgact ggtgtataag gtgtcatctc tgacgacaac
     1501 ctggatcgag atacgcgggt tcagggacac cggactgaac tcaccgggag gcacccggca
     1561 ccatgtacat gatgatacag atacgcagtg tcagcccctc tccggagggg ctttttatct
     1621 gaatgattct gttattttcg cccgtatggg catatttcca gaatgcagcg atgattaaaa
     1681 cacttatctg tgatatttcc tgtgtttgca gggcactcct ggctgttttt aattaaattc
     1741 cagacgtttt tattaaatgg ggccacgttg taaatggtga cggggagctc tttattattg
     1801 agtattacgc ctgtccgggt tggtgtaaaa atacagcccg gagggaggaa ggtatctgac
     1861 tgataccata atattaattt tatgttacac catatggctg aaaatgatat gccacatgct
     1921 ggctggatta ccgtgtcaat cactatccag ttcatttgct ttccttattt tcttaaggtt
     1981 ttaaatgatg ccataaatta taggtgatgt tttgaaagca atctgtcagg ctgagttgtt
     2041 tttctggtgt cgtttatt
//
